LOCUS       DAZKGF010000001        92389 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZKGF010000001 DAZKGF010000000
VERSION     DAZKGF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 92389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.001"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(47..1297)
                     /gene="icd"
                     /locus_tag="ACHOQV_000001"
     CDS             complement(47..1297)
                     /gene="icd"
                     /locus_tag="ACHOQV_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFP9628436.1"
                     /translation="MESKVVVPAQGKKITLQNSKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1469..2122
                     /gene="rluE"
                     /locus_tag="ACHOQV_000002"
     CDS             1469..2122
                     /gene="rluE"
                     /locus_tag="ACHOQV_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFP9628437.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTEDALEALRNGVTLNDGPTLPAGAEMVEEPEWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATD"
     gene            2132..2593
                     /gene="nudJ"
                     /locus_tag="ACHOQV_000003"
     CDS             2132..2593
                     /gene="nudJ"
                     /locus_tag="ACHOQV_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFP9628438.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2647..3753
                     /gene="mnmA"
                     /locus_tag="ACHOQV_000004"
                     /gene_synonym="trmU"
     CDS             2647..3753
                     /gene="mnmA"
                     /locus_tag="ACHOQV_000004"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP9628439.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYDGEVCLGGGIIEQRLPLPV"
     gene            3789..4430
                     /gene="hflD"
                     /locus_tag="ACHOQV_000005"
     CDS             3789..4430
                     /gene="hflD"
                     /locus_tag="ACHOQV_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFP9628440.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4434..5804
                     /gene="purB"
                     /locus_tag="ACHOQV_000006"
     CDS             4434..5804
                     /gene="purB"
                     /locus_tag="ACHOQV_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP9628441.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5973..6644
                     /gene="phoP"
                     /locus_tag="ACHOQV_000007"
     CDS             5973..6644
                     /gene="phoP"
                     /locus_tag="ACHOQV_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFP9628442.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6644..8104
                     /gene="phoQ"
                     /locus_tag="ACHOQV_000008"
     CDS             6644..8104
                     /gene="phoQ"
                     /locus_tag="ACHOQV_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFP9628443.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8180..9301
                     /gene="roxA"
                     /locus_tag="ACHOQV_000009"
                     /gene_synonym="ycfD"
     CDS             8180..9301
                     /gene="roxA"
                     /locus_tag="ACHOQV_000009"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFP9628444.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETSWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9447..10676)
                     /gene="pepT"
                     /locus_tag="ACHOQV_000010"
     CDS             complement(9447..10676)
                     /gene="pepT"
                     /locus_tag="ACHOQV_000010"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP9628445.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            10926..12062
                     /gene="potA"
                     /locus_tag="ACHOQV_000011"
     CDS             10926..12062
                     /gene="potA"
                     /locus_tag="ACHOQV_000011"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFP9628446.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPADEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFTVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12046..12903
                     /gene="potB"
                     /locus_tag="ACHOQV_000012"
     CDS             12046..12903
                     /gene="potB"
                     /locus_tag="ACHOQV_000012"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFP9628447.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12900..13694
                     /gene="potC"
                     /locus_tag="ACHOQV_000013"
     CDS             12900..13694
                     /gene="potC"
                     /locus_tag="ACHOQV_000013"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFP9628448.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13691..14737
                     /gene="potD"
                     /locus_tag="ACHOQV_000014"
     CDS             13691..14737
                     /gene="potD"
                     /locus_tag="ACHOQV_000014"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFP9628449.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14795..15256
                     /gene="ymfA"
                     /locus_tag="ACHOQV_000015"
     CDS             14795..15256
                     /gene="ymfA"
                     /locus_tag="ACHOQV_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFP9628450.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPYSKKTRRKRKSRP"
     gene            15253..16038
                     /locus_tag="ACHOQV_000016"
     CDS             15253..16038
                     /locus_tag="ACHOQV_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFP9628451.1"
                     /translation="MKKIIILLSLLILLPLTVTSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVVVPGVNEASFRQNGKAMLAHAASSMSNVKGSVLLLFTTRE
                     PRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDLPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAVA
                     NMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(16164..16985)
                     /gene="cobB"
                     /locus_tag="ACHOQV_000017"
     CDS             complement(16164..16985)
                     /gene="cobB"
                     /locus_tag="ACHOQV_000017"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFP9628452.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(17001..17912)
                     /gene="nagK"
                     /locus_tag="ACHOQV_000018"
     CDS             complement(17001..17912)
                     /gene="nagK"
                     /locus_tag="ACHOQV_000018"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFP9628453.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17941..19185)
                     /gene="lolE"
                     /locus_tag="ACHOQV_000019"
     CDS             complement(17941..19185)
                     /gene="lolE"
                     /locus_tag="ACHOQV_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFP9628454.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19185..19886)
                     /gene="lolD"
                     /locus_tag="ACHOQV_000020"
     CDS             complement(19185..19886)
                     /gene="lolD"
                     /locus_tag="ACHOQV_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFP9628455.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19879..21078)
                     /gene="lolC"
                     /locus_tag="ACHOQV_000021"
     CDS             complement(19879..21078)
                     /gene="lolC"
                     /locus_tag="ACHOQV_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFP9628456.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21340..22413
                     /gene="ycfT"
                     /locus_tag="ACHOQV_000022"
     CDS             21340..22413
                     /gene="ycfT"
                     /locus_tag="ACHOQV_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9628457.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTYVKEVPLR
                     RHLLMTSLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGTN
                     TIAIYTTHRILVEIFSLTLLAPMNAARWSPQVELTLLMVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            22541..25987
                     /gene="mfd"
                     /locus_tag="ACHOQV_000023"
     CDS             22541..25987
                     /gene="mfd"
                     /locus_tag="ACHOQV_000023"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP9628458.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26131..27093
                     /gene="ldtC"
                     /locus_tag="ACHOQV_000024"
     CDS             26131..27093
                     /gene="ldtC"
                     /locus_tag="ACHOQV_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFP9628459.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27176..27433)
                     /gene="bhsA"
                     /locus_tag="ACHOQV_000025"
                     /gene_synonym="ycfR"
     CDS             complement(27176..27433)
                     /gene="bhsA"
                     /locus_tag="ACHOQV_000025"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFP9628460.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            27674..28306
                     /gene="comR"
                     /locus_tag="ACHOQV_000026"
     CDS             27674..28306
                     /gene="comR"
                     /locus_tag="ACHOQV_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFP9628461.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(28368..28907)
                     /gene="ycfJ"
                     /locus_tag="ACHOQV_000027"
     CDS             complement(28368..28907)
                     /gene="ycfJ"
                     /locus_tag="ACHOQV_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP9628462.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(29134..30438)
                     /gene="ndh"
                     /locus_tag="ACHOQV_000028"
     CDS             complement(29134..30438)
                     /gene="ndh"
                     /locus_tag="ACHOQV_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFP9628463.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(30838..31380)
                     /gene="ycfP"
                     /locus_tag="ACHOQV_000029"
     CDS             complement(30838..31380)
                     /gene="ycfP"
                     /locus_tag="ACHOQV_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFP9628464.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(31403..32428)
                     /gene="nagZ"
                     /locus_tag="ACHOQV_000030"
     CDS             complement(31403..32428)
                     /gene="nagZ"
                     /locus_tag="ACHOQV_000030"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFP9628465.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAREAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHSDPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(32439..33263)
                     /gene="thiK"
                     /locus_tag="ACHOQV_000031"
     CDS             complement(32439..33263)
                     /gene="thiK"
                     /locus_tag="ACHOQV_000031"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFP9628466.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(33244..33885)
                     /gene="lpoB"
                     /locus_tag="ACHOQV_000032"
     CDS             complement(33244..33885)
                     /gene="lpoB"
                     /locus_tag="ACHOQV_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFP9628467.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPIEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(33899..34276)
                     /gene="ycfL"
                     /locus_tag="ACHOQV_000033"
     CDS             complement(33899..34276)
                     /gene="ycfL"
                     /locus_tag="ACHOQV_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFP9628468.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTLPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(34279..34638)
                     /gene="hinT"
                     /locus_tag="ACHOQV_000034"
     CDS             complement(34279..34638)
                     /gene="hinT"
                     /locus_tag="ACHOQV_000034"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFP9628469.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            34972..37161
                     /gene="fhuE"
                     /locus_tag="ACHOQV_000035"
     CDS             34972..37161
                     /gene="fhuE"
                     /locus_tag="ACHOQV_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFP9628470.1"
                     /translation="MLSTQFNRDNQYQTTSKPSLLAACIALALLPSAAFAAPATEETV
                     IVEGSATTPVDDENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWAYIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(37221..38654)
                     /gene="ptsG"
                     /locus_tag="ACHOQV_000036"
     CDS             complement(37221..38654)
                     /gene="ptsG"
                     /locus_tag="ACHOQV_000036"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFP9628471.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(38949..39746)
                     /gene="ycfH"
                     /locus_tag="ACHOQV_000037"
     CDS             complement(38949..39746)
                     /gene="ycfH"
                     /locus_tag="ACHOQV_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9628472.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPQDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(39757..40761)
                     /gene="holB"
                     /locus_tag="ACHOQV_000038"
     CDS             complement(39757..40761)
                     /gene="holB"
                     /locus_tag="ACHOQV_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP9628473.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFHGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(40758..41399)
                     /gene="tmk"
                     /locus_tag="ACHOQV_000039"
     CDS             complement(40758..41399)
                     /gene="tmk"
                     /locus_tag="ACHOQV_000039"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9628474.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATLPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(41389..42411)
                     /gene="yceG"
                     /locus_tag="ACHOQV_000040"
     CDS             complement(41389..42411)
                     /gene="yceG"
                     /locus_tag="ACHOQV_000040"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFP9628475.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKLRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(42414..43223)
                     /gene="pabC"
                     /locus_tag="ACHOQV_000041"
     CDS             complement(42414..43223)
                     /gene="pabC"
                     /locus_tag="ACHOQV_000041"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFP9628476.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLELEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSRPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(43343..44584)
                     /gene="fabF"
                     /locus_tag="ACHOQV_000042"
     CDS             complement(43343..44584)
                     /gene="fabF"
                     /locus_tag="ACHOQV_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP9628477.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(44672..44908)
                     /gene="acpP"
                     /locus_tag="ACHOQV_000043"
     CDS             complement(44672..44908)
                     /gene="acpP"
                     /locus_tag="ACHOQV_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9628478.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(45119..45853)
                     /gene="fabG"
                     /locus_tag="ACHOQV_000044"
     CDS             complement(45119..45853)
                     /gene="fabG"
                     /locus_tag="ACHOQV_000044"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFP9628479.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ITDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(45866..46795)
                     /gene="fabD"
                     /locus_tag="ACHOQV_000045"
     CDS             complement(45866..46795)
                     /gene="fabD"
                     /locus_tag="ACHOQV_000045"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9628480.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(46811..47764)
                     /gene="fabH"
                     /locus_tag="ACHOQV_000046"
     CDS             complement(46811..47764)
                     /gene="fabH"
                     /locus_tag="ACHOQV_000046"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP9628481.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(47832..48902)
                     /gene="plsX"
                     /locus_tag="ACHOQV_000047"
     CDS             complement(47832..48902)
                     /gene="plsX"
                     /locus_tag="ACHOQV_000047"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP9628482.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(48983..49156)
                     /gene="rpmF"
                     /locus_tag="ACHOQV_000048"
     CDS             complement(48983..49156)
                     /gene="rpmF"
                     /locus_tag="ACHOQV_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9628483.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(49208..49729)
                     /gene="yceD"
                     /locus_tag="ACHOQV_000049"
     CDS             complement(49208..49729)
                     /gene="yceD"
                     /locus_tag="ACHOQV_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFP9628484.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            49928..50512
                     /gene="yceF"
                     /locus_tag="ACHOQV_000050"
     CDS             49928..50512
                     /gene="yceF"
                     /locus_tag="ACHOQV_000050"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFP9628485.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(50625..51584)
                     /gene="rluC"
                     /locus_tag="ACHOQV_000051"
     CDS             complement(50625..51584)
                     /gene="rluC"
                     /locus_tag="ACHOQV_000051"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFP9628486.1"
                     /translation="MKTETPSVKIVAINADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            52157..55342
                     /gene="rne"
                     /locus_tag="ACHOQV_000052"
     CDS             52157..55342
                     /gene="rne"
                     /locus_tag="ACHOQV_000052"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFP9628487.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERAEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQVAEPQPEVAETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            55476..55769
                     /locus_tag="ACHOQV_000053"
     CDS             55476..55769
                     /locus_tag="ACHOQV_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP9628488.1"
                     /translation="MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            55766..56260
                     /locus_tag="ACHOQV_000054"
     CDS             55766..56260
                     /locus_tag="ACHOQV_000054"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9628489.1"
                     /translation="MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIDR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(56355..57308)
                     /gene="flgL"
                     /locus_tag="ACHOQV_000055"
     CDS             complement(56355..57308)
                     /gene="flgL"
                     /locus_tag="ACHOQV_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFP9628490.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(57320..58963)
                     /gene="flgK"
                     /locus_tag="ACHOQV_000056"
     CDS             complement(57320..58963)
                     /gene="flgK"
                     /locus_tag="ACHOQV_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFP9628491.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDASGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDTGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(59029..59970)
                     /gene="flgJ"
                     /locus_tag="ACHOQV_000057"
     CDS             complement(59029..59970)
                     /gene="flgJ"
                     /locus_tag="ACHOQV_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFP9628492.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTSMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(59970..61067)
                     /gene="flgI"
                     /locus_tag="ACHOQV_000058"
     CDS             complement(59970..61067)
                     /gene="flgI"
                     /locus_tag="ACHOQV_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFP9628493.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(61079..61777)
                     /gene="flgH"
                     /locus_tag="ACHOQV_000059"
     CDS             complement(61079..61777)
                     /gene="flgH"
                     /locus_tag="ACHOQV_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFP9628494.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(61830..62612)
                     /gene="flgG"
                     /locus_tag="ACHOQV_000060"
     CDS             complement(61830..62612)
                     /gene="flgG"
                     /locus_tag="ACHOQV_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFP9628495.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(62783..63538)
                     /gene="flgF"
                     /locus_tag="ACHOQV_000061"
     CDS             complement(62783..63538)
                     /gene="flgF"
                     /locus_tag="ACHOQV_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFP9628496.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQTTRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(63558..64763)
                     /gene="flgE"
                     /locus_tag="ACHOQV_000062"
     CDS             complement(63558..64763)
                     /gene="flgE"
                     /locus_tag="ACHOQV_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFP9628497.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMTLVFNANGVLTSDPTANISTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(64788..65483)
                     /gene="flgD"
                     /locus_tag="ACHOQV_000063"
     CDS             complement(64788..65483)
                     /gene="flgD"
                     /locus_tag="ACHOQV_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFP9628498.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGTTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(65495..65899)
                     /gene="flgC"
                     /locus_tag="ACHOQV_000064"
     CDS             complement(65495..65899)
                     /gene="flgC"
                     /locus_tag="ACHOQV_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFP9628499.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(65903..66319)
                     /gene="flgB"
                     /locus_tag="ACHOQV_000065"
     CDS             complement(65903..66319)
                     /gene="flgB"
                     /locus_tag="ACHOQV_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFP9628500.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            66475..67134
                     /gene="flgA"
                     /locus_tag="ACHOQV_000066"
     CDS             66475..67134
                     /gene="flgA"
                     /locus_tag="ACHOQV_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFP9628501.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            67210..67503
                     /gene="flgM"
                     /locus_tag="ACHOQV_000067"
     CDS             67210..67503
                     /gene="flgM"
                     /locus_tag="ACHOQV_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFP9628502.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            67508..67924
                     /gene="flgN"
                     /locus_tag="ACHOQV_000068"
     CDS             67508..67924
                     /gene="flgN"
                     /locus_tag="ACHOQV_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFP9628503.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNAANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(67964..69499)
                     /gene="murJ"
                     /locus_tag="ACHOQV_000069"
     CDS             complement(67964..69499)
                     /gene="murJ"
                     /locus_tag="ACHOQV_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFP9628504.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(69609..70532)
                     /gene="yceM"
                     /locus_tag="ACHOQV_000070"
     CDS             complement(69609..70532)
                     /gene="yceM"
                     /locus_tag="ACHOQV_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9628505.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVRAQRIVDKIWRDAMSE"
     gene            complement(70534..71181)
                     /gene="yceH"
                     /locus_tag="ACHOQV_000071"
     CDS             complement(70534..71181)
                     /gene="yceH"
                     /locus_tag="ACHOQV_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFP9628506.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVMDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(71192..71776)
                     /gene="rimJ"
                     /locus_tag="ACHOQV_000072"
     CDS             complement(71192..71776)
                     /gene="rimJ"
                     /locus_tag="ACHOQV_000072"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9628507.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            72012..73220
                     /gene="mdtH"
                     /locus_tag="ACHOQV_000073"
     CDS             72012..73220
                     /gene="mdtH"
                     /locus_tag="ACHOQV_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFP9628508.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            73284..73931
                     /gene="grxB"
                     /locus_tag="ACHOQV_000074"
     CDS             73284..73931
                     /gene="grxB"
                     /locus_tag="ACHOQV_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFP9628509.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPIELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            74065..74625
                     /gene="yceB"
                     /locus_tag="ACHOQV_000075"
     CDS             74065..74625
                     /gene="yceB"
                     /locus_tag="ACHOQV_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9628510.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            74731..75777
                     /gene="pyrC"
                     /locus_tag="ACHOQV_000076"
     CDS             74731..75777
                     /gene="pyrC"
                     /locus_tag="ACHOQV_000076"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP9628511.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVRKEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            75851..76096
                     /gene="dinI"
                     /locus_tag="ACHOQV_000077"
     CDS             75851..76096
                     /gene="dinI"
                     /locus_tag="ACHOQV_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFP9628512.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            76386..76640
                     /gene="bssS"
                     /locus_tag="ACHOQV_000078"
     CDS             76386..76640
                     /gene="bssS"
                     /locus_tag="ACHOQV_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFP9628513.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            76755..77873
                     /gene="solA"
                     /locus_tag="ACHOQV_000079"
     CDS             76755..77873
                     /gene="solA"
                     /locus_tag="ACHOQV_000079"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFP9628514.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            77921..78034
                     /locus_tag="ACHOQV_000080"
     CDS             77921..78034
                     /locus_tag="ACHOQV_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFP9628515.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVYYF"
     gene            78295..78861
                     /gene="yceJ"
                     /locus_tag="ACHOQV_000081"
     CDS             78295..78861
                     /gene="yceJ"
                     /locus_tag="ACHOQV_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP9628516.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHQAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            78865..79440
                     /gene="yceI"
                     /locus_tag="ACHOQV_000082"
     CDS             78865..79440
                     /gene="yceI"
                     /locus_tag="ACHOQV_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFP9628517.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(79482..80534)
                     /gene="trhO"
                     /locus_tag="ACHOQV_000083"
     CDS             complement(79482..80534)
                     /gene="trhO"
                     /locus_tag="ACHOQV_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP9628518.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALDVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            80759..81679
                     /gene="lpxL"
                     /locus_tag="ACHOQV_000084"
     CDS             80759..81679
                     /gene="lpxL"
                     /locus_tag="ACHOQV_000084"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFP9628519.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKTLKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            81851..83077
                     /gene="mdtG"
                     /locus_tag="ACHOQV_000085"
     CDS             81851..83077
                     /gene="mdtG"
                     /locus_tag="ACHOQV_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFP9628520.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            83160..83534
                     /gene="msyB"
                     /locus_tag="ACHOQV_000086"
     CDS             83160..83534
                     /gene="msyB"
                     /locus_tag="ACHOQV_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP9628521.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(83535..83762)
                     /gene="yceK"
                     /locus_tag="ACHOQV_000087"
     CDS             complement(83535..83762)
                     /gene="yceK"
                     /locus_tag="ACHOQV_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP9628522.1"
                     /translation="MRLIVMSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(83934..86477)
                     /gene="mdoH"
                     /locus_tag="ACHOQV_000088"
     CDS             complement(83934..86477)
                     /gene="mdoH"
                     /locus_tag="ACHOQV_000088"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFP9628523.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(86470..88005)
                     /gene="mdoG"
                     /locus_tag="ACHOQV_000089"
     CDS             complement(86470..88005)
                     /gene="mdoG"
                     /locus_tag="ACHOQV_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFP9628524.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            88399..89556
                     /gene="mdoC"
                     /locus_tag="ACHOQV_000090"
     CDS             88399..89556
                     /gene="mdoC"
                     /locus_tag="ACHOQV_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFP9628525.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(89564..91045)
                     /gene="clsC"
                     /locus_tag="ACHOQV_000091"
     CDS             complement(89564..91045)
                     /gene="clsC"
                     /locus_tag="ACHOQV_000091"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFP9628526.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGIFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(90987..91520)
                     /gene="ymdB"
                     /locus_tag="ACHOQV_000092"
     CDS             complement(90987..91520)
                     /gene="ymdB"
                     /locus_tag="ACHOQV_000092"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFP9628527.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(91615..91926)
                     /gene="ymdA"
                     /locus_tag="ACHOQV_000093"
     CDS             complement(91615..91926)
                     /gene="ymdA"
                     /locus_tag="ACHOQV_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFP9628528.1"
                     /translation="MFRPFLDSLMLGSMFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGRTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(92047..92379)
                     /gene="csgC"
                     /locus_tag="ACHOQV_000094"
     CDS             complement(92047..92379)
                     /gene="csgC"
                     /locus_tag="ACHOQV_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFP9628529.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
BASE COUNT        22477 a        24391 c        22993 g        22528 t
ORIGIN      
        1 aacgggcgtg ttatacgccc gtttcaatat ttaacacatg tagagattac atgttcttga
       61 tgatcgcatc accaaactcg ctgcatttca gcagtttagc gccttccatc agacgttcga
      121 agtcataggt tacagtcttg gcgttgattg cgccttccat acctttaaca atcaggtctg
      181 cggcttcagt ccagcccata tggcgtaaca tcatctcagc ggagagaata atagagccag
      241 ggttaacttt gtcctgaccg gcatatttcg gcgcagtacc gtgggtggct tcaaacaggg
      301 cgcattcgtc accgatgttt gcgcctgggg cgataccgat accgccgacc tgtgcagcca
      361 gagcgtcaga aatgtagtca ccgttcaggt tcatacaggc gataacatca tattcagccg
      421 gacgcagcag gatctgttgc aggaatgcat cagcaatcac atctttaatg acgatctctt
      481 tgccggtatt cgggttttta actttcagcc acgggccgcc gtcgatcagt tcaccgccaa
      541 actcttcacg cgccagctgg tagccccaat ctttaaacgc gccttcggtg aacttcatga
      601 tgttgccttt gtgcaccagg gtcacagagt cacgatcgtt cgcaattgcg tattcgatcg
      661 ctgcacgcac cagacgtttg gtgccttctt cagaacacgg cttaataccg ataccgcaat
      721 gttccgggaa acgaattttc ttcacgccca tctcttcacg caggaattta atcactttct
      781 cggcgtcggc agagtctgct ttccattcga tacccgcata aatgtcttcc gagttttcac
      841 ggaagataac catatcggtc agttcagggt gtttaaccgg gcttggggtg ccctggtagt
      901 aacgtaccgg acgcaggcag atgtagagat ccagttcctg gcgcagggca acgttcagag
      961 agcgaatacc gccaccaacc ggagtggtca gcgggccttt aatggcaacg cgatattcac
     1021 gaatcagatc aagggtttca gcaggcagcc agacatcctg accataaacc tgtgtggatt
     1081 tttcaccggt gtaaatttcc atccaggaga ttttacgctc gcctttatag gctttctcga
     1141 ctgcagcgtc gaccactttc agcatggctg gggttacatc tacaccgatt ccatcacctt
     1201 caatgtaagg gataatcgga ttttcaggaa cgttgagttt gctgttttgc agggtgatct
     1261 tcttgccttg tgccggaaca actactttac tttccattca cctctccttc gagcgctact
     1321 ggtttgctcg tctgccacca cgttgcatat gcgtttgcgt cctgcgatac ggatgcttta
     1381 gagcaatttt ttgttaatga tttgtaattg gcttgtcaat actaccctat tgtttgccgt
     1441 catgaaagat gcgcgttatt aggctataat gcggcaattc ataatctctg aaaataccat
     1501 gcaaaaaact tcttttagaa atcaccaggt taagcgattc agctcgcaac gttctaccag
     1561 gcgtaaacct gaaaaccagc ccacgcgtgt gatcctgttc aataaaccct acgatgttct
     1621 tccgcagttc accgatgaag ccggacgcaa aacattaaaa gaattcatcc cggttcaagg
     1681 cgtttatgca gcaggtcgcc ttgaccgcga tagcgaaggg ttactggtgc tgaccaataa
     1741 cggcgcgttg caggcgcgtt taacccagcc gggtaaacgt accgggaaaa tctattatgt
     1801 acaggtagaa ggtattccca cggaagacgc ccttgaagcg ttgcgcaatg gcgtaacctt
     1861 aaatgatggc cctaccctgc ccgcaggtgc agagatggtt gaggagccgg aatggttatg
     1921 gccccgtaat ccgccaattc gtgaacgcaa aagtattccc accagctggc tgaagatcac
     1981 cttatatgaa ggacgaaatc gccaggtgcg ccgcatgacc gcccatgttg gtttccccac
     2041 gctgcgactg attcgctatg cgatgggtga ttactctttg gataatcttg ccaatggcga
     2101 atggcgagaa gcgacagatt aaggacgcaa aatgtttaaa ccgcacgtta ccgttgcctg
     2161 cgtggtgcac gcagaaggca aatttttagt cgttgaagag acgattaatg gtaaagcgtt
     2221 atggaaccaa cctgccgggc atctggaagc ggatgagacg ctagtggaag ctgctgcgcg
     2281 tgagctgtgg gaagaaaccg gcatcagcgc gcagccgcaa cactttattc gtatgcatca
     2341 gtggattgcg ccagataaaa cgccgttttt gcgtttcctg tttgctattg agcttgagca
     2401 aatatgcccg acccagcctc atgacagcga tatcgactgc tgccgttggg tcagcgccga
     2461 agaaatttta caggcgtcaa atctgcgttc gccgctggtg gcggaaagta ttcgttgtta
     2521 tcaaagcggg caacgctatc cgctggagat gattggcgat tttaactggc cttttacaaa
     2581 gggtgtcatc taaaaggctg cgtgatagaa tacgccgcct tgaagttcaa tgtcgtgagt
     2641 gatccaatgt ctgaaaccgc aaaaaaagta atcgtcggca tgtccggcgg tgtcgattcc
     2701 tccgtttctg cctggctgtt gcaacaacag ggatatcagg tcgaaggcct gtttatgaag
     2761 aactgggaag aagacgacgg tgaggaatat tgcacagcgg cagcggatct ggctgatgcc
     2821 caggctgtct gcgacaagct cggcattgaa ctgcacaccg ttaactttgc tgccgagtac
     2881 tgggacaacg tcttcgaact gttccttgcc gaatataaag ccggtcgcac gccgaatccg
     2941 gatattctgt gcaacaaaga gatcaaattt aaagccttcc tcgaatttgc cgccgaagat
     3001 ttaggtgccg attatatcgc taccggtcat tacgtacgtc gggcagatgt cgatggcaag
     3061 agccgcctgc tgcgtggtct ggacagcaat aaagaccaga gctacttcct ttatacgctc
     3121 agccatgagc agattgcgca aagcctgttc ccggtcggcg aactggaaaa accgcaggtg
     3181 cgtaagattg ctgaagatct tggtctggtc accgcgaaga aaaaagactc taccggcatt
     3241 tgcttcattg gcgaacgtaa attccgcgag ttcctgggcc gttatctccc ggcgcaaccg
     3301 ggcaaaatca ttaccgtcga tggcgatgaa attggtgaac accaggggct gatgtatcac
     3361 accctcggtc agcgtaaagg tctgggtatt ggtggcacca aagaaggcac cgaagaaccg
     3421 tggtatgtgg tcgataaaga cgtcgaaaac aacattctga ttgttgcgca gggccatgaa
     3481 cacccgcgtc tgatgtctgt cggtctgatt gcccagcagt tgcactgggt cgatcgcgaa
     3541 ccattcaccg gcactatgcg ttgcacggta aaaacccgct atcgccagac cgacattccg
     3601 tgcaccgtca aggcgctgga cgatgatcgc attgaagtga ttttcgatga gccggttgcc
     3661 gccgtgacgc cgggccagtc tgccgtattc tatgacggtg aagtgtgcct cggtggcggt
     3721 attattgagc agcgtctgcc gctgccggtc tgattatttt ctttacttaa aacaaggaag
     3781 cagtgaacgt ggcaaagaat tactatgaca tcaccctcgc tctggccggt atttgtcagt
     3841 cggcacgcct ggtgcaacaa ctcgctcacc aggggcattg tgatgccgat gcgctacacg
     3901 tctcactcaa cagtattatt gatatgaacc ccagctcgac gctggcggtt tttggcggta
     3961 gcgaagccaa cctgcgcgtc gggctggaaa ccttgctcgg cgtgctcaat gccagcagtc
     4021 gccagggctt aaacgctgag ttaacccgct acacactcag cctgatggtg cttgagcgca
     4081 aactctcctc agcgaaaggt gcgctcgaca ctctgggcaa ccggatcaac ggcctgcaac
     4141 gccagctcga acacttcgat ttacagtccg aaacgctgat gagcgcgatg gctgctatct
     4201 atgttgatgt gattagcccg cttggcccgc gcattcaggt caccggttcc cctgctgtac
     4261 tgcaaagccc acaagtgcag gcgaaagttc gcgctaccct gctggcaggc attcgcgccg
     4321 ccgtgctctg gcaccaggtc ggcggcggac gtctgcaact gatgttttct cgtaatcgcc
     4381 tgaccactca ggcaaaacaa attcttgctc atttaacccc ggagttgtga tctatggaat
     4441 tatcctcact gaccgccgtt tcccctgtcg atggacgcta cggcgataaa gtcagcgcgc
     4501 tgcgcgggat tttcagcgaa tatggtttgc tgaaattccg tgtacaagtt gaagtacgtt
     4561 ggctgcaaaa actggccgcg cacgcagcga tcaaggaagt tcctgctttt gctgccgacg
     4621 caatcggtta ccttgatgca attgtcgcca gtttcagcga agaagatgca gcacgcatca
     4681 aaaccatcga gcgtaccact aaccacgacg ttaaagcggt tgagtatttc ctgaaagaaa
     4741 aagtggcgga gatcccggaa ctgcatgcgg tttctgaatt catccacttt gcctgtactt
     4801 cggaagatat caataacctc tcccacgcgt tgatgctgaa aaccgcgcgt gatgaagtga
     4861 tcctgccgta ctggcgtcaa ctgattgatg gcattaaaga tctcgccgtt cagtatcgcg
     4921 atatcccgct gctgtcccgt acccacggtc agccagccac gccgtcaacc atcggtaaag
     4981 agatggctaa cgtcgcctac cgtatggagc gccagtaccg ccagcttaat caggtggaga
     5041 tcctcggtaa aatcaacggc gcagtcggta actataacgc ccacatcgcc gcttacccgg
     5101 aagttgactg gcatcagttc agcgaagagt tcgtcacctc gctgggtatt cagtggaacc
     5161 cgtacactac ccagatcgaa ccgcacgact acattgccga gctgtttgat tgcgttgcgc
     5221 gcttcaacac cattctgatc gactttgacc gtgacgtctg gggttatatc gcccttaacc
     5281 acttcaaaca gaaaaccatt gctggtgaga ttggttcttc caccatgccg cataaagtta
     5341 acccgatcga cttcgaaaac tccgaaggga atctgggcct ttccaacgcg gtattgcagc
     5401 atctggcaag caaactgccg gtttcccgct ggcagcgtga cctgaccgac tccaccgtgc
     5461 tgcgtaacct cggcgtgggt atcggttatg cgctgattgc gtatcaatcc accctgaaag
     5521 gggtgagcaa actggaagtg aaccgtgacc atctgctgga tgaactggat cacaactggg
     5581 aagtgctggc tgagccaatc cagacagtta tgcgtcgcta tggcatcgaa aaaccgtacg
     5641 agaagctgaa agagctgact cgcggtaagc gcgttgacgc cgaaggcatg aagcagttta
     5701 tcgacggtct ggcgttgcca gaagaagaga aagcccgcct gaaagcgatg acgccggcta
     5761 actatattgg tcgcgccatc accatggttg atgagctgaa ataagcctca aatcagtgcc
     5821 ggatggcgat gctgtccggc ctgcttatta agattatcca ctttttgttt tttcacttta
     5881 cctccccttc ccgctggttt atttaatgtt tacccccata accacataat cgcgttacac
     5941 tattttaata attaagacag ggagaaataa aaatgcgcgt actggttgtt gaagacaatg
     6001 cgttgttacg tcaccatctt aaagttcaga ttcaggatgc tggtcatcag gtcgatgacg
     6061 cagaagatgc caaagaagcc gattattatc tcaatgaaca tttaccggat attgcgattg
     6121 tcgatctcgg tttgcctgac gaggacggtc tgtcactgat tcgccgctgg cgtagcaatg
     6181 atgtttcact gccgattctg gtattaaccg cccgtgaaag ctggcaggac aaagtcgaag
     6241 tattaagtgc tggcgctgat gattacgtga ctaaaccgtt tcatattgaa gaggtgatgg
     6301 cgcgaatgca ggcattaatg cggcgtaata gcggtctggc ttcacaggtc atttcgctac
     6361 caccgtttca ggttgatctc tctcgccgtg aattatctat taatgacgaa gtgatcaaac
     6421 tgaccgcgtt cgaatacacc attatggaaa cgttgatacg caataatggc aaagtggtga
     6481 gcaaagattc gttaatgctc caactctatc cggatgcgga gctgcgggaa agccatacca
     6541 ttgatgtact gatgggacgt ctgcgcaaaa aaattcaggc acaatatccg caagaagtga
     6601 ttaccaccgt tcgcggccag ggctatctgt tcgaattgcg ctgatgaaaa aattactgcg
     6661 tctttttttc ccgctctcgc tgcgggtacg ttttctgttg gcaacggcag cggtagtact
     6721 ggtgctttcg cttgcctacg gaatggtcgc gctgatcggt tatagcgtca gtttcgataa
     6781 aaccacgttt cggctgttac gtggcgagag caatctgttc tatacccttg cgaagtggga
     6841 aaacaataag ttgcatgtcg agttacccga aaatatcgac aagcaaagcc ccacgatgac
     6901 actaatttat gatgagaacg ggcagctttt atgggcgcaa cgtgacgtgc cctggctgat
     6961 gaagatgatc cagcctgact ggctgaaatc gaatggtttt catgaaattg aagcggatgt
     7021 taacgatacc agcctcttgc tgagtggaga tcattcgata cagcaacagt tgcaggaagt
     7081 gcgggaagat gatgacgacg cggagatgac ccactcggtg gcggtaaacg tctacccggc
     7141 aacatcgcgg atgccaaagt tgaccattgt ggtggtggat accattccgg tggagctaaa
     7201 aagttcctat atggtctgga gctggtttat ctatgtgctc tcagccaatc tgctgttagt
     7261 gatcccgctg ctgtgggtcg ccgcctggtg gagtttacgc cccatcgaag ccctggcaaa
     7321 agaagtccgc gaactggaag aacataaccg cgaattgctc aatccagcca caacgcgaga
     7381 actgaccagt ctggtacgaa acctgaaccg attgttaaaa agtgaacgcg aacgttacga
     7441 caaatatcgt acgacgctca ccgacctgac ccatagtctg aaaacgccac tggcggtgct
     7501 gcaaagtacg ctgcgttctc tgcgtagtga aaagatgagc gtcagtgatg ctgaaccggt
     7561 aatgctggag caaatcagcc gcatttcaca gcaaattggc tactacctgc atcgtgccag
     7621 tatgcgcggc gggacattgc tcagccgcga gctgcatccg gtcgctccac tgctggacaa
     7681 tctcacctcg gcgctgaaca aagtgtatca acgcaaaggg gtcaatatct ctctcgatat
     7741 ttcgccagag atcagctttg tcggtgagca gaacgatttt gtcgaggtga tgggcaatgt
     7801 gctggataat gcctgtaaat attgcctcga gtttgtcgaa atttctgcaa ggcaaaccga
     7861 cgagcatctc tatattgtgg tcgaggatga tggccccggt attccattaa gcaagcgaga
     7921 ggtcattttc gaccgtggtc aacgggttga tactttacgc cctgggcaag gtgtggggct
     7981 ggcggtagcc cgcgaaatca ccgagcaata tgagggtaaa atcgtcgccg gagagagcat
     8041 gctgggcggt gcgcggatgg aggtgatttt tggtcgccag cattctgcgc cgaaagatga
     8101 ataaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg ttgcagatac
     8161 cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg attttcttga
     8221 acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt ttattgaccc
     8281 gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagtcgaca gtcgactggt
     8341 cagtcatcag gatggcaaat ggcaggtcag ccacggtcca ttcgaaagct acgatcatct
     8401 cggtgaaact agctggtcat tgttagtgca agcagtgaac cactggcatg agccgaccgc
     8461 cgcgctgatg cgaccgttcc gtgaactgcc ggactggcgt attgatgacc tgatgatctc
     8521 tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg tgtttatcat
     8581 tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga aacagcactg
     8641 cccacatccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg aagagctgga
     8701 gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg cgctggaaaa
     8761 tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg cgggaactga ttagtggatt
     8821 tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc cggatgttcc
     8881 acctcgcgct catcctgcgg acgttctgcc gcaagagatg gataaactgc gtgagatgat
     8941 gctcgaattg atcaaccagc cggaacattt taagcaatgg tttggcgagt ttatatccca
     9001 gtcacgtcat gaactggata tcgcgccgcc agagccgcct tatcagccgg atgaaatcta
     9061 cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggg ggtctgcgcg tattgcgcat
     9121 tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc cggcactgga
     9181 tgcgctcgcc agcaacattg cgctgaccgc ggagaatttt ggcgatgcgc tggaagatcc
     9241 gtcattcctc gcgatgctcg cggcgctggt caatagcgga tactggttct tcgaagggta
     9301 agtttggatt taagccggat gcggcgctga ttgcgcctta tcgtgcctac atgcactgaa
     9361 cgcctgtagg cacgataagt atgtggacca gtgtcaatat aaaggtttca tcccggtaat
     9421 tggcagtcca catacgcatc gggcaatcac gacttccgtt gcgccgtcaa ctcggcaata
     9481 cggacgatca cctgcaccgc tttttccata ccttccagag tcacaaactc atgcttacca
     9541 tgatagttgt aaccgccagt gaacaggttc gggcacggta atcccataaa cgacaactgc
     9601 gcgccgtcgg taccgccgcg gatcggtttc agttccggtt caatatcgca gtcacgcatc
     9661 gcctgctggg cgatatcgag aatatgcgga tgctcaacca ctttctcgcg catattgtag
     9721 taactgtctt caatcaccag ttcaatgtag caatcaggat gtaacccttt gcccactttt
     9781 ttggcgatct ccatcatttt acgtttacgc gcttcaaact gtttacggtc gaaatcacgg
     9841 atgatgtagt gcatatcggc ccgttcaacg gtgcctttca tgctcgccag gtgatagaaa
     9901 ccttcatagc cttctgtcat ttccgggctt tcatccgccg gaacttccgc atgaatacgt
     9961 gccgccagcg acagcgcatt taccattact cctttcgccg tgcccgggtg aacattgtta
    10021 ccgacaattt tgatattgac cgacgcggcg ttgaagtttt caaactccag ttcgcctacg
    10081 ccaccaccat caacggtgta agcccagcgg gcatcgaagg cgtcaacatc aaaatgcttc
    10141 gcccctttgc ccacttcttc atccggggta aaggcgacgc gaatatcacc atgcggaatg
    10201 tttttttgtt gcaataccgc cagcgcggtc atgatttctg caatacctgc tttgtcatcg
    10261 gcacctaaca aggttttacc atcggtggta atcagcgtct gacccagtag ctgatgcagc
    10321 accgggaaca taaccggtga taaaacttca tcgccgatac ccagcgcaat atcgccaccg
    10381 cgatagtttt caacaatttg cggattcaca tttttgccgc tgcaatccgg tgaggtatcc
    10441 acatgagaaa taaagccaat cgccgggata tcgccaggga cgttagccgg taacgtcgcc
    10501 atcaaagtgc ccttctcact taaggtcaca ttgataagcc ccatctcttc gagctgctct
    10561 ttcagcagat gcaataactt ccattggcct tccgtgctgg gaacctgtct cacccctgcc
    10621 tttgattggg tatccagaga cacgtagttc aaaaatcgct caagtagttt atccatgtag
    10681 tcaccctcac tttttgtgac aacattatta agaagcaaga aaagacaaat attgcgtcag
    10741 gtcactttta gccctgcaag cgggaatatt tatcagcatt accttaatga atataaagct
    10801 aagccagtaa ttcacaacaa agattggcga ttcaagcggt ttgccgtaga attaccgccc
    10861 tcattaagag tcaccaaggt ggttaaccac aaaccccgca tcggtaagcc atccgttgcg
    10921 tttacatggg acagagtaaa aaattgaata aacaaccgag ttcgctttca ccgctggtgc
    10981 aattggcggg aattcgcaaa tgctttgatg gtaaagaggt cattccccag ctggatctga
    11041 ctatcaacaa tggcgagttc ctcacgctgc ttggcccttc tggctgcggt aaaacaaccg
    11101 ttcttcgcct gattgcaggt ctggaaactg ttgattccgg acgcatcatg ttggataacg
    11161 aggacatcac ccacgttccg gcggaaaacc gctacgtgaa cactgttttc caaagctacg
    11221 cacttttccc ccacatgacc gtgttcgaaa atgtggcctt tgggttgcgc atgcaaaaaa
    11281 cccccgctga tgaaattacg ccccgcgtga tggaagccct gcggatggtg cagctggaaa
    11341 ccttcgctca acgcaaaccg catcagctct ctggtggtca acagcaacgc gtcgccattg
    11401 cccgcgcggt ggttaacaag cctcgtctgt tgttgctgga tgagtcgctc tcagcgctgg
    11461 attacaaact gcgcaagcaa atgcagaacg agctgaaagc gttacagcgt aagcttggta
    11521 ttacattcgt gttcgtaact cacgaccagg aagaagcact taccatgtcg gacaggatcg
    11581 tggtgatgcg cgatggtcgc attgagcaag acggcacgcc gcgtgaaatc tacgaagagc
    11641 cgaaaaacct gtttgttgcc gggtttattg gcgaaatcaa tatgtttaac gccactgtca
    11701 tcgaacgtct cgacgagcag cgcgtacgcg ccaacgttga aggccgcgaa tgtaatatct
    11761 acgttaactt caccgttgaa ccggggcaaa aactgcatgt tctgctgcgc ccggaagact
    11821 tacgtgttga agagattaac gacgacaacc acgccgaagg gctgattggt tacgttcgcg
    11881 agcgtaacta caaaggcatg acgctggagt cggttgttga gctggaaaat ggcaagatgg
    11941 tgatggtcag cgaattcttc aatgaagacg atcctgactt tgaccactct ctcgaccaaa
    12001 aaatggccat taattgggta gaaagctggg aggtcgtact ggctgatgaa gaacacaagt
    12061 aagttccaga atgtagtgat tgtcactatt gtcggttggc ttgtgttgtt tgtctttctg
    12121 cccaacctga tgatcattgg caccagcttt ttgacccgcg acgacgccag tttcgtcaaa
    12181 atggtcttta cgctggataa ctacacgcgt ctgctcgatc cgctctattt tgaagtgcta
    12241 ttgcactcgc tgaatatggc gctgatcgcc accctcgcct gcctggtgct gggctatcct
    12301 tttgcctggt ttctggcgaa gctgccacac aaggtgcgtc cgctgctgct gtttctgctg
    12361 attgttccgt tctggaccaa ctcattgatt cgtatttacg ggctgaaaat tttcctcagc
    12421 accaaaggct atctcaacga gtttttgctc tggctgggcg ttatcgatac accaattcgc
    12481 atcatgttca cgcctagtgc ggtgattatc ggtctggttt acattctgct gccgtttatg
    12541 gtgatgccgc tgtactccag tatcgaaaaa ctggataagc cgttgcttga ggcggcgcgc
    12601 gatctcggtg ccagcaagtt gcagaccttt atccgcatca ttatcccact aacgatgccg
    12661 ggaattattg ccggatgtct gctggtgatg ctgccagcga tgggcctgtt ctatgtgtcc
    12721 gacctgatgg gcggtgcgaa aaacctgctg atcggtaacg tcatcaaggt acagttcctc
    12781 aatattcgtg actggccgtt tggtgcagcc accagcatta cgctgactat cgtaatgggc
    12841 ctgatgttgc tggtttactg gcgcgcttct cgtctgctga ataagaaggt ggaactcgaa
    12901 tgatcggtcg actgcttcgc ggcggtttta tgaccgctat ctacgcgtac ctgtatatcc
    12961 caatcattat tttgattgtg aactccttta acagctcgcg ctttggcatc aactggcagg
    13021 gttttaccac caaatggtat agcctgctga tgaacaacga cagcctgtta caggcagcgc
    13081 agcattcact aacaatggcg gtgttttcgg cgacgtttgc tacgcttatc ggttcactga
    13141 cagcagttgc gctgtaccgt tatcgctttc gtggtaagcc gttcgttagc ggaatgctgt
    13201 ttgtggtgat gatgtcacca gatatcgtga tggcgatttc gctgctggtg ctgtttatgc
    13261 tgctgggtat tcagcttggc ttctggtcgc tgctgttctc gcatatcacc ttctgcctgc
    13321 catttgtggt ggtgacagtg tattcgcgcc tgaaaggttt tgacgtgcga atgctggaag
    13381 cggcgaaaga tctcggtgcc agcgaattta ccattctgcg aaaaatcatt ctgcctctgg
    13441 caatgccagc ggtagcggcg ggctgggtgt taagctttac cctgtcgatg gacgacgtgg
    13501 tggtttcttc gtttgtcacc gggccgagtt atgaaattct gccgttaaaa atttattcga
    13561 tggtcaaagt cggcgtatcg ccggaagtta acgcgctggc aaccatatta ctggtgctgt
    13621 cgctggtgat ggtaattgcc agccagctta ttgctcgtga taaaacgaaa ggtaacacag
    13681 gggacgttaa atgaaaaaat ggtcacgcca cctgctcgcg gcgggtgctc tggcactggg
    13741 catgagcgcc gctcacgccg atgacaacaa cacgctgtat ttctacaact ggaccgagta
    13801 cgtgccgcca ggactgcttg aacagttcac caaagaaacc ggtattaagg ttatctattc
    13861 gacttacgag tcgaacgaaa ccatgtacgc gaagctgaaa acgtacaaag acggtgccta
    13921 tgacctggtg gttccttcaa cctattacgt cgataaaatg cgtaaagaag ggatgatcca
    13981 gaagatcgac aagtcgaagt taagcaattt cagcaacctc gatccagaca tgctcaacaa
    14041 gccgtttgac ccgaataacg actattccat tccgtatatc tggggtgcaa cggcgattgg
    14101 cgttaacggt gatgcggtgg atccgaaatc tgtcaccagc tgggccgatc tgtggaagcc
    14161 tgagtacaaa ggcagcctgc tgctgaccga cgatgcccgt gaagtgttcc agatggcgct
    14221 gcgtaagctg ggctactccg gtaacaccac cgatccgaaa gagattgaag ctgcatacaa
    14281 cgagctgaaa aaactgatgc caaacgtggc ggcgtttaac tccgataacc ccgctaaccc
    14341 gtatatggaa ggcgaagtta acctcggcat gatctggaac ggttctgctt tcgttgcacg
    14401 ccaggcgggt acgccaattg atgtggtgtg gccgaaagaa ggcggcattt tctggatgga
    14461 cagcctggcg atcccggcaa atgccaaaaa caaagaaggc gcactgaaat tgatcaactt
    14521 cctgctgcgc ccggatgtgg caaaacaggt tgctgaaact atcggttatc caacgccaaa
    14581 ccttgcggcg cgtaagctgt taagtccgga agtggcgaac gataaaacgc tctacccgga
    14641 tgctgaaacc attaaaaatg gtgaatggca gaatgacgtt ggcgcagcca gcagcattta
    14701 tgaagagtat tatcagaagc tgaaagcagg acgttaattc agcaagcggg cggtggtatt
    14761 accgcccgtt ttttatttag caaggatgtg gctaatgagc caggacagta gagttttctt
    14821 tcgtatattc ttcggtatcg gtctggtact gatcctcatt tctgttgtta tcttttataa
    14881 ccagttcacc tatagcaaag acgcgattca tactgaaggc gtcattgttg ataccgtctg
    14941 gcactccagt cattctcatc gcacgggtaa aaatggttcg tggtatcccg tcgttgcctt
    15001 tcgtccgacg ccagattaca cgctaatttt caattcaagt attggcagcg atttttatga
    15061 agacagtgaa ggcgataaag ttaatgttta ttattctcca ggacatcctg aaaaggctga
    15121 gataaataac ccgtgggtta atttttttaa atgggggttt atcggcatta tgggcgttat
    15181 ttttatctcc gtcggtttat taatcagtat gccctactct aaaaagacac gcaggaaacg
    15241 gaagtcccgc ccatgaaaaa aatcataata ttgctcagtt tactgatact cctgcctctg
    15301 acggtaacca gtaagccatt gattccaata atgaagactc tttttacgga tgttaccggt
    15361 actgtaccgg atgccgaaga gatcgcgcgt aaagccgaac tttttcgtca acaaaccggg
    15421 attgcgccgt ttatcgtggt tgtacctggc gttaatgaag caagcttcag gcaaaatggc
    15481 aaagcgatgc tggcgcatgc ggcatcttca atgagtaatg taaaaggaag tgttctgtta
    15541 ctttttacta cccgcgagcc acggttaatt atgataacca acggccaggt tgaaagcagc
    15601 atggacgata aacatctcgg ccttctggta gaaaatcaca ctctggctta tttacatgca
    15661 gatctctggt atcagggaat caataatgca ttggctgttt tacaagcaca gatattaaaa
    15721 caaccgacgc cgccactgac atattatccg catcccggac aacagcagga gaatgatctt
    15781 cccggcagta ccacgacatt aggccttttt gcctgggcag ttgcattcat cgtctttgct
    15841 gcatttttta actacacaac gcgcctttat tatgcgttaa aatttgcagt ggctatggct
    15901 gttgccaata tgggctatca ggctttatgt ttgtatatcg ataacagctt tgcgattacc
    15961 agaatatcgc cgctctgggc agggctgatt ggtgtctgta cgtttattgc cgcgttacta
    16021 tggacgagta aacggtaaaa gaggcagtaa agttgccgga tgcggcgcga acgccttatc
    16081 cggcccacaa aacccgcaaa ttcaattaat tgcgcccctt cacaggcctg acaagcgtag
    16141 cgcatcaggc aatttttccc gctttataat cccttcagca acttttcaac aaactctggc
    16201 accacctggc ttgccggacc gtaatatttc tcggcaaatt cattaccaac ctgactcggt
    16261 tcaagattca gttccacggt gtgcgcgcca tgcagtttcg cttcgtgaac aaacccagcc
    16321 gccggataaa catgccccga agtaccaata gcaatgaaaa tatcggccat cgacaacgcc
    16381 atataaattt catccatgcc gagtggcatt tcgccaaacc acactacgtg cgggcgcaag
    16441 ggtgccggga actggcaaca atggcattta tcttctgggg taacatctcc ggtccagtcg
    16501 agaacctgac cactttgcga acagcgaact ttcagcagtt ccccatgcat atgaatgaca
    16561 ttggtattac ctgcgcgttc atgcaggttg tcgatattct gcgtcaccag caaaaagcga
    16621 tcgccgaggg cgtcttgcag tttcgccagc gcaagatgcg cggcgttagg ctgaatttct
    16681 ggctgctgca gctgtcgacg acgggcgtta taaaacgttt gcaccagttc aggatcgcga
    16741 tcgaaacctt ccggcgttgc cacatcttca acccgatgtt cttcccacag gccatctgcg
    16801 gcgcgaaagg tacgaatacc tgattccgca gaaatccctg cccctgtcag tacgagtact
    16861 cttggttttt ccattgcttc cggcaccact ttatctctga aaaaaatacg ctgacgcaaa
    16921 cgctcgcgca gacggcgttt atttttacga aaacgactta accgatgacc ccgacgcgac
    16981 agcatagcaa cctctgttgt ttaatcggtt agatgtagga aggccgcacc acgcattcct
    17041 cccgcatcac cgtggcgcgc gcgttcaatg cgcggaacac gagctacagg taagagatga
    17101 cgaggcagcc tgtccgccag ttgcgttgtg attgccggga aattcgataa gccaccacca
    17161 atgacgacca ggtcagggtc aacaatggtc aggatatttc ccagacaaac cgctaataaa
    17221 tccagataac gctcaacgtg cgcccttgcc tgctcatcac cttgatcata aagcgcaata
    17281 atttcgggag cctgcaacgg ttgatgataa tagtgttgat acagccacgc aaaaccgcga
    17341 ccagacagat aattttcaat gcagccatgc tgaccacagc cgcagcggcg taacgggaaa
    17401 tccagcccca tcatggttaa cgcatcaacc ggcagacgca tatggccaaa ctcgccggta
    17461 atatagcttt taccggtaat gggtttaccg ttgaaaatca gcccgccgcc aacgccggtg
    17521 ccgagaatca accccatcac cagtggatat tgagtaaatt catcatccca tgcttcagaa
    17581 agggcaaaac agttggcatc gttatcaagg cgtacatcgc gatcaagacg tgcgctcagg
    17641 tcggcacgca gcggtttacc gctggcagca gggacattgg cggcatacag cgtaccatct
    17701 tctgtttccg gcattcccgg aataccgatg ccgacagaac ctttacagcc aaaacgccgg
    17761 tcagcttcgg ctaccagctc gcacactgca tctaaaaatg cgtcatagct gtcacgcggt
    17821 gtcggcaccc gcttttccca ctgcaactgc cgaccgctat caaacacgcc aagcgcaatt
    17881 tttgttccac caatatcaaa cccgtaatac attgccgctc cttgttttaa tgtactgcct
    17941 ttactggccg ctaaggactc gcgcagggtc aatattactg gcgcgtcgcg ccggatacca
    18001 acttgccaaa agactcagca acaatgctgt gaccagtacg tagaagacgt ccagccagtg
    18061 caattccgat ggcaggaagt caataaaata gatatcgctg gagaggaact gatgaccgat
    18121 caacttttca atccactcaa taatcggggt aagttgcagt gaaacaacta cgccgataat
    18181 cacaccacac aggctaccga acagccctgc cagcaatcca taccagacaa agatggcgcg
    18241 aattaaacca tctttcgccc ccagcgttct taatactgcg atatcgccac tcttgtcttt
    18301 caccgccatc actaaggtgg agacgatgtt gaaacaggcc acgccaatca ccagtaccat
    18361 cgccagatac ataatggcgc ggatcatctg gatatcgcga tacatatagc cgtaagtacc
    18421 aatccagctt ttaatataaa catagctgtt ggtcacttca cccgcatcgc gtaccagctt
    18481 attggcgttg aaaacatccg tcattttaag ggcaatacct gacacgctgg aacccatatc
    18541 aagatattgc tgggcatccg ccagcgggat catggcaaaa ctgtgatcga gttgaccact
    18601 caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt ggctgcatca gtttatgctc
    18661 gggattcgag ttggggatca taatcgacac ccaatcgccc tgcttcactt tcagcgcatc
    18721 cgccacgcct ttgccgatga taatttgctg ttcgcccgct ttaaaattgc gccaggcatc
    18781 cccctgaaca aacgagggta atgcgctcag acgctgttcc tgttgcgggt taacgccctt
    18841 cacctggatt gcgcgcagat tggcaccact ttctaccagt ccggtgaaat tgatatacgg
    18901 cgcagcggcg gcaatacctg gcactttttg cacgttatcc agggcttcct gccagttagt
    18961 ccacggttga tccaccgcct ctatttcgcc atgcggcacc accgccagaa tgcggttatt
    19021 cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg atcaataccg ccacgccaag
    19081 ggcaatgcca atggtagaaa tgacggagat cagcgacacc atgccgccgc gccgccgtcc
    19141 gcggctaaaa cgcaggccaa tcaataacga taaaggcatc gccattactc cgcccccatc
    19201 aggctcagtt ccgccgtcag acgaccatca cgcatctcca gttggcggct catacgtttt
    19261 gccagttgca ggtcgtgagt aaccaccagg aaggcggtgc cctgcaagcg attcaattct
    19321 ccaagcaact ggaagatgct gtcggcgtta cgtgcatcga ggttaccagt aggttcatcc
    19381 gccagcacca gccgcgggtt attgaccagc gcacgggcaa tcgccacgcg ctggcgttcg
    19441 ccgccagaaa gttcagatgg gcggtgattc gcacgatgct ccagccccac cgcttttaac
    19501 atctccagcg cacggctgtt gatttcagcg ggctttttct tgccaatcag cagcggcata
    19561 gccacgtttt ccagggcagt aaaatccggc agcaggtggt gaaactgata aataaagccc
    19621 agcttctggt tgcgcagttc tgctttcgcc gccgacgaca gtttgctcat cggctgaccg
    19681 ttaaagatca catcgccgga ggttggcgta tccagcccgc ctaacaggtg cagcaaggta
    19741 cttttaccgg aaccagagct accgacaatt gccatcattt caccttcgcc cacgctgaaa
    19801 ctgacattgt gcagcacatc ggtttgcaca ctgccttcct gatagcgttt gcacaggttg
    19861 tcgcattgca acaggatctt attcataacg taaagcctca gcgggttgtg tggcggcagc
    19921 gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc atcgccacca gcgcaataac
    19981 aatgacctgt aaaggttcga tagcgaccgg cagcgccgcg ccatcaagca ggacgccgat
    20041 tatcggcatc agattattta actggctggc aagcagtgcg ccaagcgccg ctccgaggat
    20101 cgcaccgata atcccggcgc tggccccttg caccataaag accatcatga tttgtcgcgg
    20161 agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc ttctccatca ccattagccc
    20221 tagcgaggta ataatgttaa acgccgctac ggcaacaatc aggctcagca gcaagcccat
    20281 catatttttt tccatgcgta cggcctggaa cagctcgcct ttacggtcac gccagtcctg
    20341 ccatttgctg ccttcaggca gtttttgttg acttaatgag tcgactttca gcggctcatc
    20401 cagccacaaa cgccagccgg taatattgcc tgccggataa cgcatcagac gcgaggcatc
    20461 ctcaatattc accagcattt catagccatc gacttcactg ttggcggcga aagtgccaat
    20521 cacattgaac aggcgctggc ttggaatacg ccccatcggc gtgaactggc tggcagatgg
    20581 caccatcacg cggatttgat caccgcgatt gacgcccaac tgtgaggcca gctgttcacc
    20641 gaggatgaca ttatatttcc ccggctcgag gtcagtttgt ttcacattga ccagatacgg
    20701 cgttagtgga tctttttgtg ccgggtcgat acccagcatc accccgaccg ccacgctgcg
    20761 cgcgctttgc agtaccacat caccggtagt aataggtgcg acgcgattaa cgccgtccag
    20821 tttgactgcc gtttccggga gttgctgcgg gttaagagag ccatgctcag aagagagaat
    20881 tgcctgtggc atcaggccaa ggatgttgtt ttgtagctcg cgctcaaagc cgttcatcac
    20941 tgacaatact gtgaccagcg ccatcacccc gagggtaatg ccgatggtag aaagccagga
    21001 gacgaaacga ccgaagcgat ccgctgcacg cccacgcatg taacgcaggc caataaatag
    21061 agcgacaggt tggtacatga aatccgtctg gttgctgtta gcaaagacac gagtatataa
    21121 gcgaaagcgg gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    21181 tgccagcaaa tcaatcggct aaagggcctc attttctcat cggccttgta gcctgatctt
    21241 tatctgaaaa ataaccaaat acagacagat acgcattaca ccacgcgact ggcccctcag
    21301 gatcaacagc atgttaaata aaaagagcgt gttgtcctga tgaaacaaaa agagctatgg
    21361 attaaccaga tcaaagggtt atgtatttgt ctggtggtga tttatcactc ggtcattacc
    21421 ttttatccgc atctcaccac ttttcagcat ccgttatcgg aagtcctgag caaatgctgg
    21481 atctatttca atctttacct tgcacccttt cgtatgccgg tttttttctt tatatccggc
    21541 tatttgattc gccgttatat cgacagcgta ccgtggggaa attgtctcga taaacggatc
    21601 tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc gttaagtgcg
    21661 ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc cgcgtatgcc
    21721 gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagttt gtggtatctg
    21781 tatgcgttaa ttgtttattt cgtggtatgt aaaattttta gccgtctggc gctgccacta
    21841 ttcgccttgt ttgtactgat gagtgtggcg gttaatttcg ttcccacgcc gtggtgggga
    21901 atgaacagtg tgatccgcaa tttgccttat tacagccttg gcgcatggtt tggcgcaaca
    21961 ttaatgacct acgttaaaga ggtgccgttg cgccgccatc tgctgatgac ttctttgctg
    22021 accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc gctggtatcg
    22081 attgtggtga tcatgaagct gttttatcaa tacgagcaac gtttcggtat gcgctccacc
    22141 agcctgctga atgtgattgg caccaacacc attgctatct acaccaccca tcgcattctg
    22201 gtagagatat tcagcttaac tctgcttgcg ccaatgaacg cggcacgctg gtcgccgcaa
    22261 gtcgaactga cactcctgat ggtttatccc tttgtcagtt tgttcatctg tactgttgcg
    22321 ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt ctccccgcct
    22381 tcactgcccg ctgccgtcag ttactcccgc taagccatcg ccccagccgg ggcgattcaa
    22441 tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag atatgccccc
    22501 atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac aatatcgtta
    22561 tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg gcgctgcctg
    22621 cgcgacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac ttattgcacc
    22681 agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagtttaccg atcagatggt
    22741 gatgaatctg gcggactggg aaacccttcc ctacgacagt ttttcgcctc atcaggacat
    22801 tatctcctcg cgcctttcca ccctttacca gctaccgacc atgcagcgtg gcgtactgat
    22861 tgttccggtg aatacgctta tgcagcgcgt ttgcccgcac agttttctcc acggtcatgc
    22921 gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc aactggacag
    22981 cgccggttat cgccatgttg accaggtaat ggagcacggc gaatacgcca cgcgcggcgc
    23041 gttgctggat ctcttcccga tgggcagtga gctgccttat cgtcttgatt tctttgatga
    23101 tgaaatcgac agcctgcgag tgtttgacgt cgatagccag cgcacgctgg aggaagtaga
    23161 agagatcaat ctgctgcccg cgcacgaatt tccgaccgat aaagcggcaa ttgaactgtt
    23221 tcgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata tttatcagca
    23281 agtgagtaaa ggcacattac ctgccgggat cgagtactgg cagccgctat tcttcagcga
    23341 accgctgccg ccactgttca gttatttccc tgccaatacg ctgttggtga atactggcga
    23401 tctggaaaac agtgccgaac gtttccaggc tgacacgctg gcgcgttttg aaaatcgcgg
    23461 cgtcgatccg atgcgcccac tgttgccacc acaatcgctc tggctgcggg tagacgagct
    23521 cttttcagag ctgaaaaact ggccgcgtgt gcagctaaaa actgaacatt taccgacaaa
    23581 agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc aggcacaaca
    23641 aaaagcaccg ctggatgcgc tgcgtaagtt cctcgagtct ttcgacggtc cggtggtgtt
    23701 ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcgc gaattaaaat
    23761 tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt atttgatgat
    23821 tggcgctgcc gaacatggtt ttgtcgatac ggtacgtaat ctggcgctga tttgcgaaag
    23881 cgatctgctc ggtgaacgcg ttgcccgtcg tcgtcaggat tctcgccgcg ccatcaaccc
    23941 cgatacactg atccgtaacc tcgcggagct gcatattggt cagccggtgg tccatctgga
    24001 gcacggtgtc gggcgctatg ccggaatgac cacgctggaa gcgggcggta ttactggcga
    24061 gtacctgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt cgtcactgca
    24121 tctgattagc cgttacgcgg gtggcgcgga agaaaacgcc ccgctgcata aacttggcgg
    24181 cgatgcctgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg tggcagcaga
    24241 attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta aacacgatcg
    24301 cgagcagtat cagttgttct gcgacagctt cccgtttgaa accacgccgg atcaggcaca
    24361 ggccattaat gcggtactta gcgacatgtg tcagccgctg gcaatggatc gtctggtgtg
    24421 cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc tggcagtaga
    24481 taaccacaag caggtggcgg tgctggtgcc aaccaccctt ctcgcgcagc agcattacga
    24541 caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct cccgtttccg
    24601 cagcgccaaa gagcagacgc aaatccttgc ggaagtagcg gaagggaaaa tcgatattct
    24661 gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag gcctgctgat
    24721 tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag cgatgcgcgc
    24781 gaacgtggat attctgacgc ttactgcaac gccgatccca cgtacgctga atatggcaat
    24841 gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc tggcagttaa
    24901 aacctttgtc cgtgagtatg acagtctggt ggtccgggag gcgatcctgc gtgaaatttt
    24961 gcgcggagga caggtttatt atctctacaa tgatgtggaa aacatccaga aagccgccga
    25021 acggctggca gaactggtac ctgaagcgcg gattgccatc ggtcacgggc aaatgcgtga
    25081 gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg tgctggtttg
    25141 taccaccatt atcgaaaccg ggatcgacat tcctaccgcc aacaccatta tcattgaacg
    25201 cgcggatcac ttcggtctgg cacagttgca ccagttacgc ggtcgcgtcg ggcgttcgca
    25261 ccaccaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta ccgatgcgca
    25321 aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg cgctggcaac
    25381 gcacgatctg gagatccgcg gcgcaggtga actgcttggc gaagaacaaa gcggctcaat
    25441 ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgctgtcg atgcgctgaa
    25501 agccggacgc gagccgtcgc tggaagatct caccagtcag caaacagaag tcgagctgcg
    25561 gatgccgtcg ctattgcccg atgatttcat ccctgacgtg aacacgcgtc tgtcgttcta
    25621 caaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag tcgagcttat
    25681 cgatcgcttc ggcctgctgc cggatccggc gcgtaccctg ctggatattg cccgtctgcg
    25741 ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag gcggcgtgat
    25801 cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc tgcaaaaaca
    25861 gccgcagcat tatcgcctcg atggtccgac gcgcctgaag tttattcagg atttgagtga
    25921 gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag agaacgcgat
    25981 cgcttaatgg taaatcgggg gcgtttctgc gcccccatct gcaacattta caaattcttt
    26041 gcacttccct gcactatccg acactgtcac catccataat tcaggcttat ctgtttatta
    26101 caataacctt atatttatta tggatttttg gtgatgatca aaacgcgttt ttctcgctgg
    26161 ctaacgtttt ttacgttcgc cgctgccgtg gcgctggcgc taccggcaaa agccaacacc
    26221 tggccgctgc cgccagcggg cagtcgtctg gttggcgaaa acaaatttca tgtggtggaa
    26281 aatgacggtg gttctctgga agccatcgcc aaaaaatata acgtcggctt tctcgctctg
    26341 ttacaggcta accccggcgt tgatccttac gtaccgcgcg cgggcagcgt gttaacgatc
    26401 ccgttgcaaa ccctacttcc agatgcgccg cgcgaaggca ttgtgatcaa tattgcggaa
    26461 ctgcgtctct attactaccc gccgggtaaa aattcggtaa ccgtgtatcc catcggcatt
    26521 ggtcagttag gtggtgacac gctgacgccg acaatggtga ccaccgtttc agacaaacgt
    26581 gcaaacccaa cctggacgcc aacggcaaac atccgcgccc gttataaagc acagggaatt
    26641 gagttgcctg cggtagttcc ggctggaccg gataatccaa tgggccatca tgcgattcgc
    26701 ctggcggcct atggcggcgt ttatttgctc cacggtacga acgccgattt cggcattggc
    26761 atgcgggtaa gttctggctg tattcgtctg cgggatgacg atatcaaaac actctttagc
    26821 caggtcaccc caggcaccaa agtgaatatc atcaacactc cgataaaagt ctctgccgaa
    26881 ccaaacggtg cgcgtctggt tgaagtacat cagccgctgt cagagaagat tgatgacgat
    26941 ccgcagctgc tgccaattac gctgaatagc gcaatgcaat catttaaaga tgcagcacaa
    27001 actgacgctg aagtgatgca acatgtgatg gatgtccgtt ccgggatgcc ggtggatgtc
    27061 cgccgtcatc aagtgagccc acaaactctg taaagctcgg ataaaaaaat ccccgcgaca
    27121 cggcatgtcg cggggaaaaa aatcagcagt agcaggcatt aatgagggtt aatgcttatt
    27181 tataaattac tgctgttcca tggagggtat tcggaccggt tacagaagta atacggaaag
    27241 attttgcgcc catctcatcc gctttttgcg ccagctgctc ttccagcgat cccagatttg
    27301 tccccgcgtt agcactgata gtaccgactt tttgttggcc ttctggcgtt gactgaactt
    27361 cgacagccgc aaagctggca aatgacattg agcttaaaat cgccgcagcg atgagggttt
    27421 ttacgttttt cataatagtg gccttatgca gatgaatgac gtcgcaagca attacttaac
    27481 gatcgataac taaatgatag atgtgatctg gatcacatac aagatatttt ttataacaat
    27541 cattaattaa tttaaaaatc tttaaatttc agttaaatag aaagcaatta tttttatggt
    27601 ttgtggactg gtgaacggag gtgattattt ttataataac ctttcattca cagtcaccag
    27661 gtacaacggc atcatggcaa ctgactcaac acaatgtgta aaaaaaagcc gcggccgccc
    27721 aaaagtgttc gacagggatg ccgcgcttga taaggccatg aaattgttct ggcaacacgg
    27781 ttatgaagcg acttctcttg cggacctcgt cgaagcgacc ggagccaaag cgcccacgct
    27841 atacgcggaa tttaccaaca aagaggggtt attccgcgcc gtactcgacc gctatatcga
    27901 tcgttttgcc gctaagcatg aagcacagct gttttgtgaa gagaaaagcg tggagtctgc
    27961 gctggctgac tattttgctg ccatcgccaa ctgctttacc agcaaagaca ccccggcagg
    28021 ctgcttcatg atcaacaact gcaccaccct ctccccagat tcaggagata tcgccaatac
    28081 gctgaaatca cgccatgcga tgcaagagcg cactttgcag cagtttttat gtcaacgaca
    28141 agcgcgcggg gaaatcccga cccactgtga cgtgacgcat ctggcagaat tccttaattg
    28201 tattattcag gggatgtcga tcagcgcacg cgaaggtgca tcgctggaaa aactgatgca
    28261 gattgcccga acgactttgc gtttatggcc cgaactggtg aaataaggcg caaaaaaagc
    28321 ccctcagcct gagcgaatga ggggccaaat tgcagagtac agccttgtta tactttgtta
    28381 ttcaaaatca gttgcccgtt gctatctagc gggatctgcg tacccggatc gcggtccatg
    28441 cggattttgc cctgctgatc gccaatctta taggtcacat cataaccgag cattttttct
    28501 gacttgtcat acaccgtttt acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg
    28561 agagagccct ggatctggtt accggcatat ccaccaccca gcgcccccac aacagtagcg
    28621 acatctttac cgcgaccacc accaaactga tgccctatca cgccgccagc aacagcgccg
    28681 agcaccgacc cggtaatgcg attttcatcc tgcaccggtc gacgatgggt cactgtgacg
    28741 ttacgacact cctgacgcgg tgttttaacc gtttccttga ttggggttgc agaaacaacc
    28801 tgagcgtatt gcgggccacg ttcaaacacg ttcagactgg ccactgccgc tacgcccaac
    28861 gcagctgcga caccaatccc gatacccgcc aacattgatt tattcacggg aatacctcct
    28921 tctttgcagt tatgccaatc atgcaagcga aggagaaaag cagctatcag acagaagatg
    28981 aaaagcgcgg gaatggctgg aaatacttag ggattcggag gagtctgaag gaatgcctga
    29041 tgcgacactt aacgcgtctt atcaggccta ccgctcgtac catttgtcca gggcggataa
    29101 ggcgtttatg ccccatccgc cagtacaagc cgattaatgc aacttcaaac gtggacggat
    29161 aacgcggtta atactcccca ccagcatcat taatccagtt ttaaagtaac catgcagcgc
    29221 aatctgatgc attcggtata gcgagatata tacaaagcgc gcaattcgtc cttcaatcat
    29281 cattgagccg cgtgtcaggt tacccatcag gctaccgacg gtggaaaagt tcgacagcga
    29341 taccagcgaa ccgtgatctt tatactgata atttttcagc ggcttaccgt tcatctgcgc
    29401 cagaatgttg ttcattgcgc aggttgccat ctgatgtgcg gcctgagcgc gcggcggaac
    29461 aaagccccct tccggacgcg ggcatgacgc gcagtcgcca atagcgtata tgtctggatc
    29521 gcgagtggtt tgcagcgtcg gttccaccac cagctggttg atacggttgg tttcaagacc
    29581 accgatatct ttcaggaagt ctggcgcttt gatcccggct gcccacacca tcagatcagc
    29641 ctcaatatat tcgccatctt tagtgtgcag gccgccttca tcagcactgg tgaccatggt
    29701 ttgcgtcagc acgcgaacgc caagtttcgt taactcacta tgggccgcag cagagatacg
    29761 tggcggcaat gcaggcaaaa tacgttcgcc cgcttctacc agcgttacgt tcagggcttc
    29821 gttggtcaga cctttataac cgtaactgtg cagttgcttg accgcgttgt gcaattcagc
    29881 ggagagttct acacccgtcg cgccgccgcc gacaatcgca atgtttactt tgccattcgc
    29941 gcccaggttg gcggagtatt tcaggaacag attcagcatc tcctggtgga agcgacgcgc
    30001 ctggtgcggg ttatcgagga aaatgcagtt ctctttgaca cctggcgtat tgaaatcgtt
    30061 ggaggtgcta cccagcgcca ttaccagggt gtcataggcg attttacgtt ccggaaccag
    30121 caattcacct ttttcgtcgc gcagttctgc aatagtgatt gttttcgctt cacgatcaat
    30181 atcaattacg gaacccagct ggaactggaa accatgattg cgcgcatggg ccagatagct
    30241 caacgcatcg acgccttcat caagcgagcc agtcgccact tcgtgcagca gcggtttcca
    30301 caggtggctg tggttacgat cgaccagcgt aattttggct tttttcttgc gtcccagctt
    30361 atgccccagc tgtgttgcca tttccagccc accagcaccg ccgccgacaa tcacaatttt
    30421 tttcaatggc gtagtcaacg tgaccccctt aaatttatta accaattgtt aattaatggt
    30481 tattaacata gctcttaatt aacaacaggt tacagcaatg aaaatgttct tcactattga
    30541 gaataacatg aatggtgcat tggtcatacc aaaattgatg ttaatcaagt tttgttgctg
    30601 aaaagatagg cgactttacc gggcgagatc aaaaaacaga gtttattgtt tattatttga
    30661 gcgaatttgt gagagtgata tagagtgagg ggtgtatgga gtgcgttcgg taggcctgat
    30721 aagaggcgca tcaggcattg atgctcgact gtcggatgcg gcgtggacgc cttatccgac
    30781 ttacagaatt cggagcgcat caggtaccgg caaggttacc tgatgcgacg agggcattta
    30841 cccgagggtt ttgaacgcct taatgcgctg caaatgcggg gagatattct tgaatttgtg
    30901 cgtctgctct tcgtcccaga caatctcgta ataatgatgc agctcttcag atgtccgctg
    30961 gctgttaaga gcttcatcat tacgcgacaa aatcaccaaa caacgatcgc gattcttttc
    31021 gcggaagttg gtcacacact tagtggcaat atcggcatac tcttccgggc gatcaatctt
    31081 gccttccatg ttctcataag ggaacaaatt agggttgaag atcacctggc ggatatcgca
    31141 aagaaaacca atccgttccg cccagtatcc gcccagacca acgccgcaaa tcagcggacg
    31201 ctcgtcgacg ttcagttgca acattttgtc cacttctttg agcagatgct gcatatcatg
    31261 tttcggatgc cgcgtactgt agcttatcaa gcgtacatcc gggtcaataa actgcaattg
    31321 taagactttc tcgtggttac ccggactgtt agagtcaaaa ccgtgtaaat agataatcat
    31381 cgcttcctca cataagccag ggttagtgac ctgctttctc ttcctgccag cgttcatgta
    31441 actgattcag acgggcgctg atcgctttcc agcgagccga gtccatcagt tcctgtcgcg
    31501 aaaatgaacc tttatgatac aaacgtgtaa cacgttctgc cttgatcggt gacagattat
    31561 ctaacacgct gacggcccct ttacgattat tgcagaccag gatcatatcg caacccgcgt
    31621 ccagtgatgc ctgcccgcgt tcggcataac tgcccataat cgcggctcct tccatcgata
    31681 aatcgtcaga gaaaatcacg ccatcaaaac ccagttcctg acgcaaaacg gttttcagcc
    31741 aatagggaga accgctcgcc ggacgcggat cgacatcact gtagatcaca tgcgcaggca
    31801 taatggcgtc gagtttattt tcgcggatta aggaactgaa gaccgacata tctttagcgc
    31861 gaatctccgc ttgcggacgt gggtcgcacg gtgtttcttt gtgtgagtct gccgttactg
    31921 caccgtgtcc tgggaagtgt ttcccggtcg ttttcattcc ggcttcatgc ataccatcaa
    31981 taaaccggct ggcaattgcc agggcttttt cgggatcgga atgataagaa cgctcgccaa
    32041 tcgccgcgct gatatgcccg acgtccagca ctggcgcaaa gctgatatca atatccatag
    32101 caatcatttc gctggccatc aaccaacctg cctctcgcgc cagttttccg ccttcttcca
    32161 ttcccaacag cgcagcaaat gattgtgccg ctggcaagcg ggtaaaacct tcacgaaaac
    32221 gctgcacgcg tccaccttcc tgatcaaccg ccaccaccag atgattgcgc gaagctgcgc
    32281 ggatctggcg caccagttca cgtaactggg caggatcatg atagttacgc gtaaagagaa
    32341 tcagccctcc caccagcgga tgcgccagta tttcacgctc ttccgcgtcc agttcgtaac
    32401 cttcgacatc caacattact ggacccacac tgctctcctt attgttttat taatagctgc
    32461 cgccaggtgt catcggccag cctgataaat tgttgatcgc cggtttgtcg ccagcggtac
    32521 tcaaaccacc ctgctttgag catcagcagc cagggaaacc atcgcctgac ctgacgccat
    32581 aattgcgccg gataaatctt cgcgcgagtg gcatagtcat tgaccaattg ccggtgctgg
    32641 tcagtatttt ccacccacac cgccgccagt tccagcgcga tatcaccatc tccggcatac
    32701 tcccagtcga tgagttttaa ccctgacgcg ctatgcacta aatttccggc gtggacatcc
    32761 atatgcaatg gacttaagcg taaaggccgt ggttcccgcg ctttgcgcag acgttttaac
    32821 cttcgcagcc aacccactgt ccgccgcgcc ggatcgcttt gctgccagta cagttccagt
    32881 aacggcaaca gcgttattcg ccagccaaaa cgcggttgtt gatgtagata atacagcaag
    32941 cctgccagtt cgttggtatc cggcaaatac gtttttacct cgccgggcag atagtcgacc
    33001 accatccagt cacggagata taaatgcggc ttcggtgcaa tgcatgcggg tagttgtgat
    33061 aaagcccgat actggcgcaa gaacgcgaac cgcggcgcat caggatcgtg cggctgacgc
    33121 acaacaaaac gctggccctg atgttcaatg agaaaactcc cgccactaag cccactatta
    33181 aacgtcgtga cgggatgaaa ctgcgggaaa aagcgcgaca gcaattcgtc gcgcgtgatg
    33241 ggattattgc tgcgaaacgg cacctttacc tgaccagata atttcgcccg tctgcaccag
    33301 catcagctgc atttgaaggg tcggagcgtt aacattgcca gaggcgctgg agtacagcac
    33361 gtaatgagcg ccgacattgc gggcaatgcc tatggcttta ctacgggtgc ctaaactgtc
    33421 ctgcggcgac aaacctaact gttgctttgc catcgacagc tgctgggcgg aaaccagggt
    33481 aaatttaccg ttattagcca gcgcatttcg cagcgtttcg gtcgcttctg cggcattcag
    33541 cgaaccgtta gtacggttgt taacgctatc aaccagcagg acgctacccg cagtcacgcc
    33601 gtcagcccca agcatcttac tgaccatcgg ctgcattgcg ccattccagt cataatggcg
    33661 aatatgcggc gcaggcggtg cagtttgatc ttcgtgctca attgggcctg gctgctgcgg
    33721 tatcgtcggc accgatggca ctgtggggac aggctgttgc ggctcggctg gttgttccgg
    33781 cgctggtttc acttcttcta tcggcgcagg ttcacgttgc cccacacacc cggcgagaaa
    33841 catcgccagc gcggtaatca aggcgtagcg actcattttg gtcatcaaga ttcacccctt
    33901 acaaatatag ataaagtctg actttgtgcg cccccagaaa attggcgctg ccgtacagcg
    33961 ttaccgccga atgtgcggga agggtaacgc tgcgtggcct ttccagagga tgcatctcca
    34021 gccctctggc gtcataccag taaaaacgat aatgaacggt aacgggttct tgcctttcgt
    34081 tataaagcgt tgaggatgct gaaggttgaa tatcagacgt cgaaaggacg ggcttttctg
    34141 cactgatgcc cgcagccaat aaagatgact ccatcaccag cgattgctca tcattcaccg
    34201 gaatttccgg atgtgaacga cagcccacca gcactaacaa cgccagagac accagcccaa
    34261 agcatccttt tctcattgtt acagaccttt atgcgccagc attggtccca gtggacggcc
    34321 acccaacaaa tgcatatgga tgtggtaaac ctcttgtccg ccatggcggt tggtgttcat
    34381 gatcaggcga tagccatctt cggcaatacc ttcttgctca gcaatttttg ccgctacggt
    34441 gatcatgcgt cccagcgcct gctcatgctc agctgacacg tcgttcacag tcggaatgag
    34501 gatattcgga atgatcagaa tatgcgttgg cgcttgcggc gaaatatcgc gaaacgccgt
    34561 taccagatca tcctggtaga cgatatcgga ggggatctca cgacgaataa ttttgctgaa
    34621 tatagtttct tctgccacga cattttcctt tttcaaatag ccatgcgttg cgccatgcta
    34681 cgccgcgcca cactgcgagt atagagtatg gtcgagttaa acctgctctt tcaaccttaa
    34741 ctcacgattt cttaagcaaa aaatcgtttt gctgccgtat atatcgccat tattcccatt
    34801 tctgcctgcg aatgcgctga aaagacattc tctgtttggt ttacacgtca aatatgaatg
    34861 cgtatatttc tcatttgcat ttacatgcaa aattattcgc acactaaata taaccgctca
    34921 tcaccctcct ccggcatgag cctgacgaca acataaacca agagatttca gatgctttca
    34981 acacaattta acagggataa tcaatatcaa accacctcca aaccgtcact gcttgccgcc
    35041 tgcatagcac tggcactctt accttccgcc gcttttgctg caccagccac tgaagaaacg
    35101 gtgattgttg agggttcagc cactactcca gttgatgacg aaaatgatta cagcgtaacg
    35161 tctacctctg cgggtaccaa aatgcagatg actcaacgtg atattcctca gtcggtcact
    35221 attgttagtc agcagcggat ggaagatcag cagttacaaa cgctgggcga agtgatggaa
    35281 aacacgctgg ggatcagcaa aagccaagcg gattccgatc gtgctcttta ttattcccgc
    35341 ggattccaga tcgataacta tatggttgat ggtatcccca cctattttga atcgcgctgg
    35401 aatctgggcg acgcactttc tgatatggca ctgtttgaac gcgtagaagt agtgcgtggc
    35461 gcgacaggac tcatgaccgg gacgggtaat ccatctgcgg caattaatat ggttcgaaaa
    35521 cacgcgacca gtcgtgaatt taaaggcgat gtctcggcgg aatacggtag ctggaacaaa
    35581 gaacggtatg tggcggattt acaaagccca ctcaccgaag atggtaaaat ccgcgcacga
    35641 attgtcggcg gctaccagaa taacgactca tggctggacc gctacaacag tgaaaagacc
    35701 ttcttctccg gcattgtcga tgctgattta ggcgatctta cgacgctttc agccggttac
    35761 gaatatcagc gcattgatgt taatagccca acctggggcg gtttaccgcg ctggaatact
    35821 gatggcagca gcaacagtta cgatcgcgca cgcagtaccg cacctgactg ggcgtataac
    35881 gataaagaga tcaacaaggt ctttatgacc ctcaagcagc ggtttgctga tacctggcaa
    35941 gcgacactga atgccaccca ctccgaagtc gaatttgaca gtaaaatgat gtatgtcgat
    36001 gcctatgtaa acaaagcgga tggtatgctg gttgggccat acagtaatta tggacctggc
    36061 tttgattatg tcggcggtac cggttggaac agcggcaaac gtaaagttga tgcgctggat
    36121 ttgtttgctg acggtagtta tgaattgttt ggtcgtcagc acaatctaat gtttggtggc
    36181 agttacagca aacaaaacaa tcgttacttc agttcatggg cctacatctt cccggatgaa
    36241 attggcagtt tctacaactt taatggcaat ttcccacaaa ccgactggtc accacagagc
    36301 ctggcgcagg acgataccac acatatgaaa tcgttatatg ctgccactcg tgtcaccctt
    36361 gccgatccgc tgcatctgat cctcggcgca cgttatacca actggcgggt tgatacgctg
    36421 acttacagca tggagaaaaa ccacaccacg ccttacgctg gtctggtgtt tgacatcaat
    36481 gacaactggt cgacctacgc cagctatacc tctattttcc agccgcaaaa tgatcgtgac
    36541 agttcaggca aatatctggc tccaatcacc ggtaacaact acgagctggg tctgaaatcg
    36601 gactggatga atagccgtct gaccaccacg ttagccatct tccgtattga gcaggataat
    36661 gtcgctcagt ccaccggtac acctatcccc ggcagcaacg gcgaaaccgc ctataaagcg
    36721 gtggatggga cagtcagtaa aggtgtggaa tttgaactca acggcgcaat taccgacaac
    36781 tggcagctga cgtttggcgc aacgcgctat attgcagagg ataacgaagg aaacgccgtt
    36841 aatcctaatc tgccacgcac cacggttaaa atgttcacca gctaccggtt gcctgtcatg
    36901 ccagagttga cggtcggcgg tggtgttaac tggcaaaatc gcgtgtatac cgacaccgtg
    36961 acaccgtatg gcaccttccg cgccgagcaa ggtagctacg cgctggtgga tctcttcacc
    37021 cgctaccagg tgacgaaaaa cttctcgtta caggggaatg tcaataacct gttcgacaaa
    37081 acctacgata ccaacgtgga aggttctatt gtctacggcg caccgcgtaa tttcagcatt
    37141 accggcacgt atcaattctg aataacacct gtaaaaaagg cagccatctg gctgccttag
    37201 tctccccaac gtcttacgga ttagtggtta cggatgtact catccatctc ggttttcagg
    37261 ttatcggatt tagtaccgaa aatcgcctga acaccagaac cagcaaccac tacgcccgct
    37321 gcgcccagtt tcttcaggcc agcctgatcc actttagaca catcagcaac gctgacgcgc
    37381 agacgggtaa tacatgcgtc gaggttagta atgttttctt taccaccaaa tgcagcaacc
    37441 agagccggtg ccatttcgct ggtacctgtc gcttttgcgt cttcagtcgc gtcttcacga
    37501 cccggcgttt tcagatccag tgctttaatc agcacgcgga agatggtgta gtaaacaatg
    37561 gcataaccga taccgacgat cgggaacagc cacagtttgc tgctgttacc agacagaacg
    37621 atgaagtcga tcagaccgtg cgagaacgac gtaccgtcac gcatccccag aagaatacag
    37681 attgggaatg ccaggcctgc cagaatcgcg tggatgatgt acaggatcgg cgcaacgaac
    37741 atgaaggaga actcgatcgg ctcggtgata ccggtcagga acgaggtcag cgccgcggag
    37801 atcataatac cgcccacttt cgcgcggttt tctggtttag cagagtgcca gatagcaatt
    37861 gcggcagctg gcagaccgta cattttgaac aggaagccac cagacagttt acccgcagtc
    37921 gggtcacccg ccatataacg cggaatgtcg ccgtggaaaa cctgacctgc tgcgttggtg
    37981 tattcaccaa tctgcatctg gaaaggtacg ttccagatgt ggtgcagacc aaacggtacc
    38041 aggcaacgtt cgatgaaacc gtaaatgcca aacgcaacta ccgggttctg gtaagcagcc
    38101 cactgagaga aggtctggat tgcagaacca atcggcggcc aaatgaagga cagcacaacg
    38161 ccagtaaaga tggcagccag gcctgaaatg atcggcacaa agcgtttacc ggcaaagaag
    38221 ccaagatact caggcagctt aatacggtag aaacggttaa acatgtacgc tgcgatcgca
    38281 ccggagataa tccccccgag tacgccagta tccgccaggt gtttagaggc gatttcttca
    38341 gcaggtaaat gcagtaccag tggcgcaacc acggccatgg ttttaaccat gatgccatag
    38401 gcaacaactg cggccagcgc ggatacgcca tcgttattgg taaagccgag ggcgacaccg
    38461 atcgcaaaaa tcagtggcat gtttgcaaag acggaaccgc ctgcttctgc cataacatgc
    38521 gatacaacgg cgggcagcca gctgaaattc gcggaaccga cgcccagcag aatacctgcg
    38581 ataggcagta cggataccgg cagcatcagc gatttaccga ccttttgcag gttagcaaat
    38641 gcattcttaa acataattga gagtgctcct gagtatgggt gcttttttta cgttttcacg
    38701 cgtggcaagg ggggagagcc tcgccgtgta cagggcatct aagcgccctt tatttattac
    38761 acagagtaaa ataattcagt gccgatttgt ttgacggcta tcacgtttca acttcagatc
    38821 attggagaag ttgcacattt tgacaaaaca tatgtcacaa tatttcaaac gtggttattc
    38881 accgcccaat tttaggcggt gaactttact cgttttagcc attaattacg agctttaaaa
    38941 aaactcattc aacggatgga ttgaaggcgg gaagcgtcga tgtgaaacag acgggcgaag
    39001 ttatcggtgg ttacctgcgc cagttcttca acggcaacac ctttcaacac agccatgtat
    39061 tctgcaacgt cacgaaccat cgcaggttga ttctcttttc ctcgatgcgg aaccggtgca
    39121 aggtaaggtg agtcagtttc taccagtaac cgatcctggg ggacataacg cgcggcatcg
    39181 cgcagttgct ccgcattacg gaaggtcaca atgccggaaa aggagatgta aaatccgaga
    39241 tccagtaatt tacccgccgt ttctctgtcc tctgtaaaac agtgtagtac gccaccgcaa
    39301 tccgtcactt tttcttcgcg cagaattgcc agcgtatcgg cgcgggcgtc acgggtatgg
    39361 acgataaccg gcttgttcag ttcacgacca atctggatat gatggatgaa ggattcttgc
    39421 tgccgtactt tagtttccgg cgtgtaataa taatccaacc cggtttcacc cagcgcaaca
    39481 acgccctctt ctgccgccag acggcgtaaa tcttctacat cgtagggatc attctggttg
    39541 agtggatgca cgccacaaga aaatacgacg ttgtcacgtt cgcctaccag atcccgcatg
    39601 tgtaaataac ccggtaatgt tgtggcgact gccagacaaa atttcacatc gcgtgcggcg
    39661 gctttcgcca gaacgtcatc cacgtcctta tgcaaagatt cataatccag accatcgaga
    39721 tggcagtgtg agtcgactaa aaacatgatg tctctcttaa agatgaggaa ccggtagcac
    39781 aacgcccggt tgcaggtaat gctcaatacg cagtaaaaga tcggtgatga gaagctcgcg
    39841 gttaatacct gtgacagaca ttaactgttc acgaatgtgg caaacatccc ccagtatagc
    39901 ctgcaggcgc gagggagaaa gatggtttgc cagttcgacg accaggcccg gcacatcaac
    39961 attggtcacc tgcgcagcgc catgatggcg ttttagcgca tccatcagca acgttgccag
    40021 ccagtgtaaa cgcgccggag cttgttcatg attaagggcc gctagcagcg aataccagtc
    40081 gcccgatggc acgctatatg ccaaagcctg acacaatgtt tcacgagcct gccagttatc
    40141 tccgtgaaac aacgccagtg ccgcgccagg cgaaccggca cttaagcgca atgcggcaag
    40201 taatgcatcc tgtgacattg tcacttcgcg tgaaagccag gtcacggcgt actgttccgg
    40261 cggcggcgca aggtaatgta accgacaacg actacgtaat gttgccagta aacgttctgg
    40321 ctcgcgggta gcaaggaaaa accacgtttc tgctggtggc tcttcaagcg ttttcagcaa
    40381 tgcgttagcc gcggcgtcgg tcagtaaggc agcatcggtt acccagacaa ctttcgcacc
    40441 acctaagcgt gcgtgctcat tcagcttttc ggtgacctca cgtaccgcat caatgcccag
    40501 cgtatttttc cctttttcgg gagccagggt gtagtaatcg ggatgcgtgc cagcctgcat
    40561 caactgacat ccacgacagt gaccgcaact tttgtggccc tgcggttgtt ggcagagtaa
    40621 ataacggctc agggcgtaga ttaaagcatc atcgcccatg cccggtaacg cctgaatgag
    40681 cagcgcatgg tgacctcttc cggcctgata gctggctacc agtttttcga aatcaggtcg
    40741 taaccatgga taccatctca tgcgtccaac tccttcaccc agttggtcac ggtagtgcgg
    40801 attgcatcca tcacagcctc cagcggcagg gtggcatcaa tggtatgaat gcttttatct
    40861 tgtgctgcca gttccagata gcgggcgcgg gtgcgattaa aaaaatcgaa agactcttgc
    40921 tcaatacgat ccagctcgcc gcgtgcacgc gcgcgtttta agccaacttc cggggtaaca
    40981 tcgagataga gcgttaagtc ggggcgaaaa tccccgagaa cagcatcacg cagtgttgcc
    41041 agcatatgtt ggtcaatacc acgtccgccg ccctgatacg cctgagtgga gagatcgtgg
    41101 cgatcgccaa tcacccaagt gccgttagcc agcgcaggtt tgatgaccgt ttctaccagt
    41161 tgaacgcgcg cggcataaaa catcagaact tcggctttat cggtaatgac ttcatcgcct
    41221 accgatttga tatccagcac caggcttctt aacttttcgg caagttgcgt accgccaggt
    41281 tcccgagtga aaaccatgtc gcggataccc agttgctcaa gcgtctcaac caccacgtta
    41341 cgcgcggtag ttttgcctgc gccttccagc ccctcaatga cgatatactt actgcgcatt
    41401 tttttcctta agcactttca gataatcctg cacagacttg ttatgactgg caagattggt
    41461 attaaacgtg tgaccacctt taccatcggc cacaaaatag agatacggcg tttttgccgg
    41521 atgcgcagca gccttcagcg aatccgcccc cggcgtcgct atcgcacctg gcggcaaacc
    41581 ggtaatggta taggtgttat acgctgtcgg cgtttccagg tctgcacgag aaagtttgcc
    41641 attataacgc tctcccatcc cgtaaatcac ggtcgggtcg gtctgcaggc gcataccaat
    41701 gcgtaaccgg ttgataaata ccgaggcaac ctgatcgcgt tcactggcaa cggcggtttc
    41761 tttttcgata attgatgcca tcgtcaccaa ctggttttta tctttataag gcagaccgtc
    41821 cgcacgccct tcccaggcgc tatcgaccgc tttcaccatt ttcttgtgcg ctcgcttgag
    41881 taacgcgaca tcggtggtat tggcggtata catccaggtg tctggccaga accaaccttc
    41941 aatccactcc gggttttcca gttcaagtgc ctgcgctacg gtggcgtact tatcgtcgct
    42001 cagcgtgtgc ttgatatagg gggcctcacg caattgcttg aggtaatcgc tcagacgcat
    42061 cccttctacc agtcgcagag ggaactgtgc ttctttaccg ctttccagca atttcagcat
    42121 ctcgcgcacg gtcatctgcg gtgtaaagcg gtaagtcccg gctttaaagt gagaaagatc
    42181 cggttcgata cgcagcagcc attggaaaac ccgtgggcga ttgatgatct tatcggcata
    42241 aagctgttca ccgagcgcca gacgtccggt ccctggcttc agggtaaata tcgtctcttc
    42301 tttgataagt aatttgctgt cggcaagatg gcgaagcttc cagacgccca caccagcggc
    42361 gatacccagt accaccagca ataacaagat aatcaataac acttttttca tgactaattc
    42421 gggcgctcac aaagtggggc taaatattca tataacgttg ctgacgaaaa ggagacatcg
    42481 ccacaggcac gtacgggcat cactggcatt aacgcattac aaataaccat ctcatctgcc
    42541 tgcaacgcct cctccagcga ggcttgcact tcgacaagct gataagagga ttgtgccagc
    42601 aaacggatac agaattgtcg cataatgccg ttaacacctg cctgatccag tcgcggcgta
    42661 taaactacgt tgcccttccg ccagaacaaa ttagccgcac agcattccgt aacccaccct
    42721 tcgctgtcaa ggaccagcgc ctcatcagcg tttgtctgct caagatgaga gcggatcaat
    42781 acttgctcaa gccgattaag atgtttaata cctgcaagat gaggattgcg ccccagccgc
    42841 accgggctta gcgccaacgt catcccctcg ttacgcaaac ggtcgtaatg tgcgggataa
    42901 accgtaacgg agagaatccg cgttgccggt ctgctgttca atgtgctgta tcctcgcccg
    42961 ccactaccgc gactgatcac cactttcagc acaccatttt gctgttctgc tgccagcgtt
    43021 ttcatctcta gttcaagctg aggccagaag tcacaggaaa tcatcaaccg ctgacaagca
    43081 tcctgtagtc gctggatatg cgccgataac aaactgactt taccgtcgat aactctggcg
    43141 gtggtaaaac aaccatcacc aaactgcgtt gcccgatcgc ttactgccag cgattcctgc
    43201 ttatgaccgt taattaagaa cataccggct ccttatcaga gaattgttag tgtggcagca
    43261 tgttcactac ggaacaagtc ggaataaaag ctaagaaaaa aggcccgcaa gcggaccttt
    43321 tataagggtg ggaaatgaca acttagatct ttttaaagat caaagaacca ttagtgccac
    43381 cgaagccgaa ggagttacac agcgtgtatt ccattccgct gacctgacgt gcttcgtgcg
    43441 gtacgaaatc cagatcgcaa ccttcatccg ggttatccag gttgatggtt ggcggaacag
    43501 cctgatcgcg cagcgccagg atggagtaga tagattctac tgcacccgcc gcacctaaca
    43561 ggtgaccggt catagatttc gtggagctta ccaacacacg gcttgcagct tcaccgaaga
    43621 tggttttcac cgcctgcgct tcagctttat cgccagccgg cgtagaagta ccgtgagcgt
    43681 taacgtagcc aatctgactc gcttcaatgc ctgcatcacg cagagcattt gccattgcca
    43741 gagctgcgcc tgcgccattt tctggcggtg acgtcatatg ataagcatcg ctgctcatac
    43801 caaagccgac gagttcagcg taaattttcg caccgcgttt tttcgcgtgt tcgtactctt
    43861 caagtaccag cataccggca ccatcgccca gtacaaaacc atcacgctct ttatcccacg
    43921 ggcggctcgc cgcttgcggg ttatcattgc gggtagataa tgcacgtgcc gcgccaaaac
    43981 caccaacgcc cagcggcgta ctggctttct ctgcgccacc tgcaaccatc acgtcagcat
    44041 cgccatacgc gataatacgc gcagcatggc caatgttgtg cacgccggaa gtacaggcgg
    44101 tcgcgataga gatgcttggg ccacgcaggc catacatgat agtcagatga cctgccacca
    44161 tgttcacaat cgttgacgga acgaagaatg ggctgatctt acgtggacca ccgttcatca
    44221 gagatgtgtg gttttcttcg atcagtccga ggccgccaat cccggagcca attgcggcac
    44281 caatgcgggt tgcgttctct tccgttattt caaggccaga atcctgcatg gcctgaacgc
    44341 cagcgacaat tccatattga atgaaggcat ccatcttgcg ctgttctttg cgcgagataa
    44401 tgtcctcaca gttaaaatcc tttactaagc cagcaaattt cgttgcatag gcgctagtat
    44461 cgaaatggtc gattaggctg atgccactct gaccggcaag cagagctttc caggtagact
    44521 ctacggtatt gccgacagga gacaacatgc ccagtccggt cacaactaca cgacgcttag
    44581 acacgtttgt cctccaggga gggaaaaaat gattctagtg ggacaaaaag ataaaactca
    44641 ggcggtcgaa cgaccgcctg gagatgttca cttacgcctg gtggccgttg atgtaatcaa
    44701 tggcagcctg aacggtggtg attttctcag cttcttcgtc cggaatctca gtatcaaact
    44761 cttcttccag agccattacc agctcaacgg tgtcaagaga atccgcgccc aggtcttcaa
    44821 cgaaagaagc attgttggta acttcttcct gcttaacgcc cagctgttcg ccgataattt
    44881 tcttaacgcg ttcttcgata gtgctcatac tcttaaattt cctatcaaaa ctcgctttcg
    44941 cgatggtttt cgtagtgtat aaaatgttga aaaatttgca actaaatccc ggcaggtctt
    45001 accacgattt tacgttattt tgaggcctta ccccccaata acgcaaatca ttttgcacta
    45061 attattactc ataaccacgc agactgcgcg caacatgagc attttgtgca aatcgcggtc
    45121 agaccatgta catcccgccg ttcacatgca aagtttcacc cgtgatgtaa gctgcttcgt
    45181 cggatgccag gaatgcaacc gcgttggcga tttcctgtgc gccgccgagg cgacccgcag
    45241 gaacctgcgc caggataccc gcacgctggt catcgctcag cgcacgtgtc atgtccgttt
    45301 caataaagcc cggagcaaca acgtttacag taataccgcg tgacgcaact tcgcgcgcca
    45361 gtgatttact gaagccgatc aagcccgctt tcgccgcagc gtagttggcc tgaccgccat
    45421 ttcccatggt accaaccaca gaaccgatag tgataatacg accatgacgc tttttcatca
    45481 tagcgcgcat taccgctttt gacagacgga aaacagatga aaggttggtt tcgataatat
    45541 cgttccactc ttcatctttc attcgcatta acaggttatc acgagtgata ccggcattat
    45601 tgaccaggat atccacttca ccaaattctg cgcgaatttt ttccagaaca gattcgatag
    45661 atgccgggtc ggtcacattc aacatcagac ctttgccgtt ggcacctaaa taatcagtga
    45721 tcgcctgagc gccattttca ctggtcgcag tgccgataac tttcgcgcca cgggctgcga
    45781 gcgtttcagc aattgcgcgg ccaattccgc ggcttgcacc ggttaccagt gcgatttttc
    45841 cttcaaaatt catgattttc ctcttttaaa gctcgagcgc cgctgccatc gctgaaggtt
    45901 cgttcagcgc cgaggcggtc agggtgtcga caatgcgttt cgtcaggcca gtaagcactt
    45961 tgcccgggcc gacttcatag agatgttcta cgccttgcgc tgccatgtat tcaacagact
    46021 tcgtccactg aaccgggtta tacaactgac gtaccagtgc gtcacggatg gcatcaccat
    46081 tggtttcgca tttcacatca acgttattca caacaggaac tgttggtgcg ttaaaggtga
    46141 ttttcgctaa ttctactgcc agtttgtcgg ctgctggttt catcagcgca cagtgagacg
    46201 gtacgctcac tggtaacggc agagcgcgtt ttgcgcccgc agctttacag gcagcgccag
    46261 cacgctcaac tgcttcttta tgaccggcaa taaccacctg tcccggagag ttaaagttta
    46321 ccggagaaac gacctgacct tctgcagctt cttcacacgc tttcgcaata gacgcatcat
    46381 ccagaccgat gattgccgcc atagcacccg ttccttccgg tacggcttct tgcatgaact
    46441 tgccgcgcat ctcaaccaga cgcaccgcat cagcgaaatc aatcacacca gcgcaaacca
    46501 gcgcggagta ttcccccagg ctgtgaccag ccatcattgc cggtgcttta ccgccctgct
    46561 gctgccatac gcgatacagc gcaacagatg cagtcaacag cgctggctgg gtttgccagg
    46621 ttttattcag ttcttcagcc ggcccctgct gggtcagcgc ccacaggtcg tagcccagcg
    46681 ccgcagaagc ttcagcaaac gtttcttcga caattggata gctcgccgcc atatcagcca
    46741 gcattccaac ggtttgagaa ccctgtccag ggaacacaaa tgcaaattgc gtcatgtttt
    46801 aatccttatc ctagaaacga accagcgcgg agccccaggt gaatccaccg ccaaaggctt
    46861 caagcagaac caactgcccc ggcttaatgc gcccgtcgcg tacagcttca tccagagcgc
    46921 acgggacaga ggccgcagag gtattaccgt ggcgatccag cgtcaccacg acattgtcca
    46981 tcgacatgcc gagttttttc gctgttgcac tgataatacg caggttagcc tgatgcggaa
    47041 ccagccagtc cagttgagaa cggtcaagat tattcgccgc cagcgtctca tctacgatgt
    47101 gcgccagttc tgttaccgca accttgaaga cttcgttgcc cgccatcgtc agatgaattg
    47161 aattctctgg attcacacgg tcagcattag gcagcgtcag caactcacca tagctaccgt
    47221 cggcatgcag atgggtggag atgattcccg gctcttcaga ggcagccagc accgcagcgc
    47281 ccgcgccatc gccaaaaata ataatagtcc cacgatcggt tggatcgcag gtgcgcgcca
    47341 gtacatcgga accgacgacc agagcatact tcaccgcccc agatttcacg tattgatcgg
    47401 ctacgcttaa tgcataggtg aaacctgcgc aggctgctgc aacgtcaaat gccgggcaac
    47461 ctttaatgcc cagcatgctt tgaatctgac aagctgcgct cgggaaagcg tgcgtagcag
    47521 aagtcgttgc cacaacgatc aggccaatct ggtctttctc aatgcccgcc atctcaattg
    47581 cgcgtgtcgc cgcttcaaag cccatggttg aaacggtttc gtttggcgcg gcaatgtggc
    47641 gttcgcggat accggtacga gtgacaatcc actcgtcaga ggtgtccacc attttttcca
    47701 aatcggcgtt tgtccgcact tgttcgggca gatagctgcc agtaccaata atcttcgtat
    47761 acatgtacgc tcagtcactt ttcggttata taccgtcact tgcaaactgc gagttcgctg
    47821 gcagcgtcct gctaccgcag agttccgctt ttgccaccgt ccagcagctc aaaaccagct
    47881 gggtatacag attccaggcg agcggcaatt cgctgaggaa cttgtcgctg caccgcctgc
    47941 actgcctgtt caatcgcgac cgcaaaagct cgctgattgg ctgcaccatg actttttatc
    48001 accgtgccgc gcaatcctaa cagacaggcg ccgttatact ggtcggggtt gaggtgactg
    48061 aatcgcctcg tcaggctctt ttgtagccaa cgctttaata acagtagcca ccacgaccgt
    48121 tttttccctt caccctgaga tttcagcaga gaaaggaaca tcctgacaac accttccatc
    48181 gtctttaatg tgacatttcc tgtaaagccg tcacaaacca gcacatctgt cttgccagtt
    48241 aacaactcat tggcttcaag atagccgata taattgatag aagggattgt tttaagcacc
    48301 gctgaggcat cccgaatact gtcgagaccc tttacttctt cttcaccaat attgagcaac
    48361 gccacgcgag gattaggaat ttccaccacc tcttcagcca gaactgagcc cataatggca
    48421 aattgcacca acattgtact gtcacaatcg acgttggccc ctaaatcgag gaccaccgtt
    48481 ttgccctttt gctgatgcgg taataccgtc accagcgccg gacgctcaat cccctccagg
    48541 ggcttgagta ataattttgc cagccccatt agcgccccgg tattaccggc actgacacag
    48601 gcttgcgctc gaccttcttt caccagctcc agtgccacgc gcattgaact cccacgactg
    48661 gcgcggatag cttgcgaagg ccgggcatca ctggcgataa ctgactgcgc aggaataatc
    48721 tgcagacgcg aacgttgttc aaagtcagct ttagcaagta atggcgtgat ggcgtcggga
    48781 ttgccgacta aaagaagagt gagttgcgaa ttagaattca gtgcctgcaa tgctgcaggc
    48841 actgtcacgg aagggccaaa atcccctccc atgacatcta acgccagggt tagacgtgtc
    48901 aaggtatcgt cgccgcccgg tttggtcttt ccccagttgc ctggggaaaa tcctcactaa
    48961 gcttcatcac gcagacgcgt gattacttag cgatgacctt gcggccgcgg tagtaaccgt
    49021 cggcagtgat gtggtgacgc aggtgttttt caccagaagt tttgtctaca gacaggctgg
    49081 tgactgcggt cagcgcgtca tgggaacgac gcatgccacg tttggaacgg gttggtttat
    49141 tctgttgtac ggccatggac cttactcctc aattacggtt tatccccgat ccaacgggga
    49201 gcaccaatta cttacgcttt aagctggcta atacggcaaa tgggtttggc ttttgcgctt
    49261 cttcaggcag ttcaccaaag accatgtccg cttcggacac ttcacagtgt tcagaatcat
    49321 gcaccggaac taccggcaag gcgaggatga tttcatcttc aaccattgca agcagatcga
    49381 tttcaccgaa ttcgttaacc tcaatcggtt catacgcttc cggcagtgct tcagcctgtt
    49441 cgtctgaacg cacaggacta aaacaatacg ttgtgtagac ctgatgagta aacggcttcc
    49501 cgcaacgctg acactcgagc gttaccgtca ccttcgcatc gccgtttaac actgcgagac
    49561 gttggttatc gatagcgaac gacatggagc attccacatc actgtccaca ctgactacgg
    49621 attcggcgac gcgctcaacc tgatcaggag tatagatacc ctggtaatca aggcgttttt
    49681 gagccgtacg aaccggatcg agagtcaggg gtaattttac cttttgcata gggcgcgcat
    49741 attaactttg taacgtcata gagtcaaaga aaaaggcagc ctgtggttgc cttttgccaa
    49801 taattcgcac acattgcggg ttactgcttt attttcgttc agtgctgata acgaacatca
    49861 acctgcaaaa cgaagcgcat agtttaaaat ggctatcatc aatacgctat atctgatgga
    49921 aaaaaatatg cctaaactta ttttagcctc cacctcgccc tggcgtcgcg ctcttctgga
    49981 aaaactgcaa atctcttttg aatgtgcagc acccgaggtc gacgaaactc cacgcagcga
    50041 cgaatcaccg cgacagttgg tgcttcgact ggcacaagaa aaagcgcaat ctctggcgtc
    50101 acgttatccg gatcatttaa ttattggttc agaccaggta tgtgttcttg atggcgaaat
    50161 caccggtaaa ccgttaacgg aagaaaatgc ccgtctgcaa ttacgcaaag ccagcggcaa
    50221 tatcgtcacc ttctatactg gactggcgtt gtttaattcg gcgaatgggc atctacaaac
    50281 agaagtggag ccttttgacg tccatttccg tcacctgagc gaggcggaga ttgataatta
    50341 cgtgcgtaaa gagcatcccc tgcactgcgc gggtagcttt aagagtgaag gatttggcat
    50401 tacgctgttt gagcgtttag aggggcgaga tcctaacacg ctggttggtt taccgctaat
    50461 cgcgctgtgt cagatgttac gtcgggaagg gaaaaacccg ctgatgggat agttttctgc
    50521 gccggatgcg gttacgtcgc atccggcaat cggtgctggc ggcctgatgc ggcgtaaacg
    50581 ccttatcaag gccaaccgcc cattatcagg tttatatgct tgtattagcg cgcgttacgc
    50641 agcttttgca aacaacgctt caaaccttca tccatcggcg cttcgatacg catcacctca
    50701 ccggtccccg gatgggtaaa cttcaacgct gcggcgtgca ggaacagacg atttaatcct
    50761 gtgcctgctt cagtgagctg tctgtcaaat tcacggtcac cgtagcgatc gtcaaaggca
    50821 atcggatgac ccgcatactg tgtatgcaca cggatctgat gagtacgccc tgttaccggg
    50881 ctacaacgca ccagggtggc aaaagcatag cgttcttcca ctttaaagcg tgtttccgac
    50941 ggtttgcctt cctgactcac acgcacgata cgttcgccgc tttgcagaat atttttcagc
    51001 aacggcgctt gaacgctctt cacatgcgac tgccactgac cgcgcaccag cgccaggtaa
    51061 tctttttgca tccctttttc acgtaattgc tcatgcagag aacgcaacgc cgagcgtttt
    51121 ttcgctacca gcaacacacc tgaagtgtcc cggtcgagac gatgaaccag ttcaaggaac
    51181 cgcgcttccg ggcgcaacgc ccgcaaacct tcgataacgc cgaagcttaa accactgccg
    51241 ccatgtaccg ccgtaccgga aggtttattc agcaccagga tatggtcatc ttcatataag
    51301 atgacgtccg ccagcgccgc caccttttgc agatgtggcg aaaccgcctc ttcttcccgt
    51361 tcagcaacgc gaaccggtgg gatacgcacc tcatcacccg cttcgagttt atattcaggc
    51421 ttaatacgtt ttttgttcac ccgcacttcg cctttacgca aaatacggta aatcatactt
    51481 tttggtacgc ctttcaattg ggtacgcaaa aagttatcga tacgttgccc cgcttcgtca
    51541 gcgttgatag caacaatttt tacggatgga gtctctgttt tcatggtggg cgattctaat
    51601 tagccaacag gattcgcgcc actcattttt ctatgcttat atttagtttt gcaccttatt
    51661 acttcactac gtgatcactt tattgatggt tattaaacca atcaccagca agaagtgaaa
    51721 aaactgtgag taagcgggtg ataaatggta aaagtcatct tgctataaca aggcttgcag
    51781 tggaataatg aggccgtttc cgtgtccatc cttgttaaaa caagaaattt tacggaataa
    51841 cccattttgc ccgaccgatc atccacgcag caatggcgta agacgtattg atctttcagg
    51901 cagttagcgg gctgcgggtt gcagtcctta ccggtagatg gaaatatttc tggagagtaa
    51961 tacccagtct gtttctttga taattgcgct gtttttccgc atgaaaaacg ggcaaccgac
    52021 actctgcgcc tctttgagct gacgataacc gtgaggttgg cgacgcgact agacacgagg
    52081 ccatcggttc acacccggaa aggcgttact ttgcccgcag cttagtcgtc aatgtaagaa
    52141 taatgagtaa gttacgatga aaagaatgtt aatcaacgca actcagcagg aagagttgcg
    52201 cgttgccctt gtagatgggc agcgtctgta tgacctggat atcgaaagtc cagggcacga
    52261 gcagaaaaag gcaaacatct acaaaggtaa aatcacccgc attgaaccga gtctggaagc
    52321 tgcttttgtt gattacggcg ctgaacgtca cggtttcctc ccactaaaag aaattgcccg
    52381 cgaatatttc cctgctaact acagtgctca tggtcgtccc aacattaaag atgtgttgcg
    52441 tgaaggtcag gaagtcattg ttcagatcga taaagaagag cgcggcaaca aaggcgcggc
    52501 attaaccacc tttatcagtc tggcgggtag ctatctggtt ctgatgccga acaacccgcg
    52561 cgcgggtggc atttctcgtc gtatcgaagg cgacgatcgt accgaattaa aagaagcact
    52621 ggcaagcctt gaactgccgg aaggcatggg acttatcgtg cgcaccgctg gcgtcggcaa
    52681 atctgctgag gcgctgcaat gggatttaag cttccgtctg aaacactggg aagccatcaa
    52741 aaaagccgct gaaagccgcc ctgctccatt cctgattcat caggagagca acgtcatcgt
    52801 tcgcgcattc cgcgattact tacgtcagga tatcggcgaa atccttatcg ataacccgaa
    52861 agtgctcgaa ctggcacgcc agcatatcgc tgcattaggt cgcccggatt tcagcagcaa
    52921 aatcaaactg tacaccggcg agatcccgct gttcagccac taccagattg agtcacagat
    52981 tgagtccgcc ttccagcgtg aagttcgtct gccatctggt ggttccattg ttatcgacag
    53041 caccgaagcg ttaaccgcca tcgacatcaa ctccgcacgc gcgacccgcg gcggcgatat
    53101 cgaagaaacc gcgtttaaca ccaacctcga agctgccgat gagattgctc gtcagctgcg
    53161 cctgcgtgac ctcggcggcc tgattgttat cgacttcatc gacatgacgc cagtacgcca
    53221 ccagcgtgcg gtagaaaacc gtctgcgtga agcggtgcgt caggaccggg cgcgtattca
    53281 aatcagccat atttctcgct ttggcctgct ggaaatgtcc cgtcagcgtc tgagcccatc
    53341 actgggtgaa tccagccatc acgtctgccc gcgctgctcc ggtaccggta ccgtgcgtga
    53401 caacgaatcg ttgtcgctct ctattctgcg tctgatcgaa gaagaagcgc tgaaagagaa
    53461 cactcaggaa gttcacgcca ttgttcctgt gccaatcgcg tcttacctgc tgaatgaaaa
    53521 acgttctgca gtgaatgcga ttgaaactcg tcaggacggc gtgcgctgtg tgattgtgcc
    53581 aaacgatcag atggaaaccc cgcactacca tgtgctgcgc gtgcgtaaag gggaagaaac
    53641 tccaacctta agctacatgc tgccgaagct gcatgaagaa gcgatggcgc tgccgtctga
    53701 agaagagttc gctgaacgta agcgtcctga acaacctgcg ctggcaacct ttgccatgcc
    53761 ggatgtgccg ccagctccaa ccccagctga acctgccgcg cctgttgtag ccccagcacc
    53821 taaagctgca acggcaacac cagcatctcc tgcacaacca gggttgttga gccgcttctt
    53881 cggcgcactg aaagcgctgt tcagcggtgg tgaagaaacc aaaccggccg agcaaccagc
    53941 accgaaagca gaagcgaaac cggaacgtca acaggatcgt cgcaagcctc gtcagaacaa
    54001 ccgccgtgac cgtaatgagc gccgcgacac ccgtagtgaa cgtgctgaag gcaacgataa
    54061 tcgcgaagaa aaccgtcgta atcgtcgcca ggcacagcag cagactgccg agacgcgtga
    54121 gagccgtcag caggctgagg taacggaaaa agcacgtacc accgacgagc agcaagcgcc
    54181 gcgtcgtgaa cgtagccgcc gccgtaacga tgataaacgt caggcgcaac aagaagcgaa
    54241 ggcgctgaat gttgaagagc aatctgttca ggaaaccgaa caggaggaac gtgtacgtcc
    54301 ggttcagccg cgtcgtaaac agcgtcagct caatcagaaa gtgcgttacg agcaaagcgt
    54361 agccgaagaa gcggtagtcg caccggtggt tgaagaaact gtcgctgccg aaccaattgt
    54421 tcaggaagcg ccagctccac gcacagaact ggtgaaagtc ccgctgccag tcgtagcgca
    54481 aactgcacca gaacagcaag aagagaacaa tgctgataac cgtgacaacg gtggcatgcc
    54541 gcgtcgttct cgccgctcgc ctcgtcacct gcgcgtgagt ggtcagcgtc gtcgtcgcta
    54601 tcgtgacgag cgttatccaa cccaatcgcc aatgccgttg accgtagcgt gcgcgtctcc
    54661 ggaactggcc tctggcaaag tctggatccg ctatccaatt gtacgtccgc aagatgtaca
    54721 ggtcgaagag cagcgcgaac aggaagaagt acaagtgcag ccgatggtga ctgaggtccc
    54781 tgtcgccgcc gctgtcgaac cggttgttag cgcgccagtt gttgaagaag tggctgaagt
    54841 tgtagaagcc cccgttcagg ttgccgaacc gcaaccggaa gtggctgaaa cgacgcatcc
    54901 tgaagtaatt gccgccgcgg taactgaaca gccgcaggtg attaccgagt ctgatgttgc
    54961 cgtagcccag gaagttgcag aacacgcaga accggtggtt gaaccgcagg aagagacggc
    55021 tgatattgaa gaagttgccg aaactgctga ggttgtggtt gctgaacctg aagttgttgc
    55081 tcaacctgcc gcgccagtcg tcgctgaagt cgcagcagaa gttgaaacgg tagccgcggt
    55141 tgaaccagag atcaccgttg agcataacca cgctaccgcg ccaatgacgc gcgctccggc
    55201 accggaatat gttccggagg caccgcgtca cagtgactgg cagcgcccta cttttgcctt
    55261 cgaaggcaaa ggtgccgcag gtggtcatac ggcaacgcat catgcctctg ccgctcctgc
    55321 gcgtccgcaa cctgttgagt aataattagc tcaagtaatc aagccctggt aactgccagg
    55381 gcttttttat ttcaggggta gcatctttgc tgtaatgaca ttagcgttac atgtactgta
    55441 taattatcat ccacgcaaag ccgaaggata tatttatgcc agcagccaat aacattccag
    55501 ccaggcgcga atctttaaac ttgcgcataa aacccgcaga acgcgatctt attgaccggg
    55561 ccgctaaagc ccgaggaaaa aatcgcaccg aatttgtcct tgaggccgcc agagccgcag
    55621 ctgaagaggc gctcattgag cagagaattt tcaggatcga ttctgatgat tatcagctat
    55681 ttttgaatcg tctggatcag ccccccgcgc ccaatgctgc attacgcaaa accatgcaaa
    55741 cacccgcgcc gtgggagcaa aaaaaatgat tcaggccccc gagccactca tggcaaggca
    55801 tcaattcacc tctttttgct cgggagtaga aacgatggat aactggctaa agcagagagc
    55861 cctaaaaaat cagcttgccg gcgcatcacg tacatttgtt agctgtgata cgtatagcaa
    55921 cgttttggca tattattcgc ttgcttccag cgccgttgaa acgtatgtcg cgacaggacg
    55981 atttcgccga aatatgccag aaccgatccc tgttgtagta cttggtcgtc tggcaataga
    56041 taaatcatta cagggtcagg gcatcgatcg tgcgatggtc agagatgccg gtctacgagt
    56101 actacaggcg gctgaagtca ttggtattcg cggaatgttg gtccatgcgt tatcagacca
    56161 ggcccgtgag ttttatctgc gagtggggtt tgaaccatcg cctgtcgatt ctatgatact
    56221 gatggcaaca ttggctgatt tgcaggaatg tcttaaatag ttcgttatgt ctcatgcaaa
    56281 aaaatgcccg ccccggcgat cccagagcag gcagacaaaa catacccagt ttcatgatat
    56341 gttttaaagc gaaattattt gttgagctgg aagagcgaca atccctgcat atcggtaaat
    56401 gctttatacg acgcctgcaa tgccgtttgc tgcatgatgt aagatgaaat agttgcattc
    56461 cagtccacat caaccagatc gctcatctgc tgcgtttgcc ctaaagcgcg atcgctacct
    56521 aatgaatcca gcgactccag ttcgttcagc tgcgtgccta attccgcgcg aacagtcagc
    56581 acattgttca gcgagttttt cagtccgcgg ttggttttat ccagtgccgc agcggcggtt
    56641 tctttatccg cttcgctatc cgcgaccggc gttttcaggg ctgcgatggc gctatccagc
    56701 atggcaaaaa gattggtttc agaagcgcta ccgtctggtt ccgctaccgc gttgctggta
    56761 atactgtcga aaattttgtc acccgtgtgc cctatcacca tcgaacgcga agcatcgacc
    56821 tgttgtttaa tactttctgc tccgccttcg tattttccat ccgcctcgct aaacggtgcg
    56881 gattctgttt tataaccggc aaaaatgtag cgcccgttac cgtcagtggt gttcgccaga
    56941 ttcagcaact ggtcacgaag cccctgaata tccgtagcca gcgaggcccg gtcatcgtca
    57001 ctcaaggtgc cattgctggc gtagacaatt ttttcctgag cattctggat agcggtggtg
    57061 acctggctaa gtacactctc ttccagtgac actttttgag tggcgaaagt acgcgccagc
    57121 gtgtactggc tgttttgcgc ctgtgcctgg gagagaacaa cagcttgtga tgcagcaatg
    57181 ggatcatctg aagggttaac gactcgctta cccgtcgaca tctgttcgcc gtacttcatc
    57241 cattctgcct gagaattggt gataccacgc atgttttgct ggtacatcat ctgggtactg
    57301 aaacgcatct tatctcccct tagcgaatgt taatcagcgc atcaaaaatc gcgtttgccg
    57361 tctgcagaac ctgcgcattc gccaggtaat actgctgaaa acgttgcaga tttccgtact
    57421 cctcatcgag attgacaccg gaaatcgact gctgctgatt ggaaagctgc gtcaccacat
    57481 taccttgcgt ggtgctactg gttttcaacg tcgcggtttt attaccgata tcactcacta
    57541 acgaggcata agcgtcgtta aaggatttcg caccgcccac cgttttactg ttgctttgca
    57601 gatcgagcag ggcctgaccg ttgcggttgt cgctatcacc tgtatcttct gcgctcgcca
    57661 tggcgatttt cgcttcgtcg gtgattaata catccatgtt gacgatggcg tcacttaccg
    57721 gtttcagcgt gaagctgtcg ttaacggcag gcgttcctgt aaacgtcaac tccagaccat
    57781 caaatgccac tttaccgctg gcatccggcg tcaccgtaaa agtggtatta ctggcaaggc
    57841 gggtgacctg ccactgatta ttatcgaacg agattttgta atctgtcgcc agtaccgcgg
    57901 aggcatcagt taccgttgca ccgatcgcaa cgtcaccttt gtttttcgtg ttttgcagaa
    57961 ccgcgggctt accgatagca aagaaatctt caccggcatc gccgttagca tcaaatccgg
    58021 ctttgtgttg ggtgttgaaa gcctcggcaa atgccagcgc cagttgtcca agcgtattac
    58081 gcgtctggtc cagatcctga gaacggaatg tcagaatgcc gcccagcgac ccggtattca
    58141 gtaatttctc cgggatctca atattgcctg ccgtcccatc aacataagcg acagtcgtac
    58201 gagaagggtc agcgctggaa ggaactgccg ccagttgccg agccgtactt ccctgaacca
    58261 gtgagtaacc attggccatc gtgatgttat aagtgccacc atcctgaacg ctgacttcta
    58321 caccaacaat ctggtttaat tcgctcacca gttgatcgcg ttgatccagc agattgttag
    58381 gtgacgcccc tgcccccacg cctgtcaggc gggagatttg atcgttcagg ctggcgattt
    58441 gtttagcgta attgttgatc tgatcaacgc tggcacctat cgcgatattg acctgtttgt
    58501 cctggtcgcg cagatattga tcggtggttt taaactgatt caccaaccct tctgatttcc
    58561 caatcagcgc ctggcgcgct gccggatctt ccgcgttact caccagcgtt tgcaggctgg
    58621 tgaagaaatc ctgcatctgt gttgccagcg aagaggtact ggtggagagc atattgtcga
    58681 ttttcgacat ctgctcatag cgggcggtca ggccgctact ttgcgtctgc gcagcacgta
    58741 actggttggt aataaacgca tcatactcac gctgcacacc agaaacgtag acgccattgc
    58801 caacccagcc gccagcgccc aacgtgctat tggcctgcgc cataatagtg gtttggcggg
    58861 tatatccggc aacgttatag ctggagatat tattacttgc cgtatttaac gccgcctggg
    58921 ccgcgttcag tccgctcatg gcgttattga tcaagctgga catggaggtt ccttataagc
    58981 cttcaattac agagtattat cggcagcgcc ccgccggact tgagttattc agaacagatt
    59041 atcaatgttc atgctgtagg ttttgctcac cttgtcgctt atcgatttca tctgctgaat
    59101 catgctggtg agtttgcggg catagtgagg atcggtggca tagcccgcgt cctgtagggc
    59161 ctgcgccccc tgctccgcac tcgcggcggt cgtcacggcg gcgtagcgcg ggttacgcgt
    59221 taacagccca acgtaatccg acaatgcttc cagatacgag ctgtagaccc gaaacttcgc
    59281 ttttactttc ttcgcttcgc cattttcata ttcagtcgtg gtgatttcag tgactggccc
    59341 tttccagttg ccagaggctt tgacgccaaa caggttatag ctcggctcgc cgttttcacg
    59401 gcggatttgc cgttgtcccc agccagattc cagcgccgcc tgagcgagga tcaaatgatg
    59461 tggcacaccg ctttgctggc ttgccagttg ggcgggcaac gagagttgcg cgaggaatgc
    59521 tttactgtca cccggcagcg aatcatcgta gttacgtggt acggcctttt gcaccagctg
    59581 cgaaagcgtc tgattttgat aacgcaccac ggtttcgagc gggaatttca tcggtgctgc
    59641 tggcgtggac tcctctggca atggttgttc tggcgtcatc tgtttaacca tcatctctgc
    59701 cagccccaga cctttgcccg ccgtcatctg ttgggcaatc tgctggtcat acatactggt
    59761 atacaggcga gtgtgctcgc tgctgaacag gccatctttt ggtaaagcgt cgcgcatgct
    59821 tttcaacatc atctgcacga acatcccttc cacctggcgg gccaccggac ggatatttgc
    59881 cgccggatct tcgcccgctt tcgccttaag ttcgttgagc gattgtgcat cccaggccgc
    59941 acttgccagt agtttgctgt cgctgatcat cagatgattt ccagttttgc ccgcagacat
    60001 cccgcacttt gcattgattg caaaatagac atcagatcca tcggcgtagc gcccagcgca
    60061 ttgagcgcac gcaccacgtt attgaggctg gcgctggaac gtacgctttg cagcgaaccg
    60121 ccgctctggc gtaaatcgat ctgcgtttgt ggagtaacca cagtctgtcc accaccaaac
    60181 ggtgtatctg gctggctgac attggcctgg cgattaactg ttactgagag atttccctgc
    60241 gctaccgcgc agctgtcgag ggtcacttcg cgattcatca ccaccgaacc ggtgcgcgag
    60301 ttaatcacta ctttagcgtc ctgcggggta acattaacct gcatattctg gatatcggca
    60361 aggaaacgga cctgggaact gttgccactc ggtacgcgca cctgaatagt ccgcgcatcc
    60421 aacgcggtgg cgctgccata tccacgcact cggttgatgg tgtcagcgat ttgctgtgcc
    60481 atgctgaaat cttcgtcgtt aagttgcaaa ttaagggtat tcccgacgcc aaactggctg
    60541 ggcaattcac gttcaataac cgcaccattg gtgatccgtc caccgttcag ttggttcacc
    60601 tgaacactgc taccgccagc ggaggcccct gcgccgccaa ccagaatatt gccctgcgcc
    60661 agcgcataca cctgactgtc aacgccctta agcggtgtca tcaataacgt accgccacgc
    60721 agacttttgg catttcccat ggaagaaacc accacgtcaa tggtttgccc ctgacgtcca
    60781 aacggtggaa gtgacgctgt caccataacc gcagcgacgt tttttagctg catattagtg
    60841 cccgtcggaa cggtaattcc cagctgtgag agcatgttat taagcgtttg tgtggtaaac
    60901 ggcgtctgag ttgtctggtc accagttcca tccagcccca ccaccaggcc atagccaatc
    60961 agtgagtttt gccttacccc ctgaacactg gtgagatcgc gaatacgctc agcctgagcc
    61021 gccgtcgtaa ccagtagaag aattaatgca gagagaaatt taatcaccac agcctcactt
    61081 acattggaga caggttaaga aagaaacgct gcaaccagcc catattttgc gcttcgttaa
    61141 tgtagccatt gcctacgtat tcaatgcgcg catccgccac ctgagtagac ggtacggtat
    61201 tgctgccgct gatagtgcgt ggattaacca cgccagagaa gcgaataaat tcggtaccct
    61261 gattaatggc gatctgtttt tcacccacca catgcaggtt gccgttaacc agtacctggt
    61321 caaccgtcac cgtcaacgtg ccgctaaagg tattactggc attggccccg ccttttccgt
    61381 tgaacgtgtt accaccggag gcttcgacat cggcacgagc gttaccaaac agcccctgca
    61441 aatagcgcgg cacagtatca aagccaaaat tagttttacc gtcacggctg gcattcgcag
    61501 aggagctttt gctggcgctg acgttctcct gcaacacgat ggtcagcgta tcgccaatat
    61561 tgcgtggtcg acgatcttca aacagcggtt gatagccata gttaatcggc tgagcagact
    61621 ggaaaataga accgttggcg acgggcgtcg gaccgggaac cggttgtgca ctggtcgccc
    61681 cctgtaccag cggcgtggag ggtatccagg cgcagccggt tagtgaaagc accaacaagc
    61741 tggaaatggc ataagtatgc gcagcgtttt tttgcatggc tatcatcttc aaaaatcagt
    61801 aaaccggtga atctgccacc ggttatgcct tagagttgcg tcagtttttg cagcatctga
    61861 tcggtggtgg ataccgcttt actgttgatt tcgtaagcgc gttgcacctg aatcatattg
    61921 accagttctt ccgccacgtt gacgttagac gtttcaacat acccttgata cagcagtccc
    61981 gcgccgttca ggcccggcgt gctttcgttc ggtgcaccgg aggattgcgt ttcggtgtag
    62041 aggttttcgc caatactctc cagtccggta tcattcataa aggtggtgag attgagctgc
    62101 ccaacctgaa ccggagctgc ctggccttgt tgtgttacgc tgaccacgcc atcacgaccg
    62161 atggtgatac ttagcgcatt cgccggaatg gtgatcgctg gctgcacctg aaaaccacct
    62221 gccgtcacca gctgcccgtt ctgatccacc tggaaagagc cgtcacgggt ataggctgat
    62281 gagccatctg gcaacatcac ctggaaaaag ccctgccctt taatcgcgac atctttgctg
    62341 ttgttggtct gcgacagatt gccctggctg tgtaagcgtt cagtggcgac cgggcgtaca
    62401 cccgtgccga tttgtaatcc ggatggtaag gtggtttgtt cggaagactg agcacctggc
    62461 tggcgaatgg tttgataaag cagatcttca aataccgcgc gctgacgctt aaaaccgttg
    62521 gtactgacgt ttgccaggtt gttggcaatg acgtccatat tggtttgctg ggcatccagg
    62581 cccgttttgg cgatccataa tgaacttatc ataaaatgtc ctgtagtgga ttagtgacaa
    62641 agtgcatctg tcttcttgcc ggatgcggcg taaacgcctt atccggccta caagagctcg
    62701 cgaaatcaat aaattgcgat tgccccatag acccgataaa cttgcgcatc gggcaactta
    62761 tacttgtcat gtatcctttc aattaactca tcgacagcag ttggttggct cggcctgcgt
    62821 tatcatcgac gctgctgatc accttcatct gcatttcaaa acgccgcgcg ctggcaatca
    62881 tgtcgctcat tgccgcaacg gcattgacgt tactgccttc cagaacaccc gacatcacac
    62941 gcaaggttgg atctgcctgc agtaccggcc cacgcgtggt ctgggtttct gcgcttaaac
    63001 gaaaaatgcc gtcgtcaccg cgctggactt cgctgcccgt ggctttcacc agtttcagac
    63061 gccccactgg cgcaaccgta tttgccggat cgcccggatt cagcgccgag attgtgccat
    63121 cggcagcaat agtgatttcc gccccttccg gcacagcaat tggcccagcc tcgcctatca
    63181 ccggatgtcc ctgaattgtc agttgcccgg tgggatcaac ctgaatgctg ccattacgcg
    63241 tatacccttc gctaccgtca gcggtctgca cggccagcca gccatcctgc tgcaacgcaa
    63301 cgtccagcgg gcgcgaggtg taatccatct tgccgggcgt catgtctgca cccggcgttg
    63361 acgccgttac cagcgtgcgc gtaggcagtg aaagcccttc caccggcact gcgcgcaagg
    63421 cgtttaactg cgcgcgaaaa ccgggcgttg aggcattggc cagattactg gcggttaccg
    63481 cctgttgatt caacgtctgg ctggctgctc ccatcgcggt ataaattgcg tgatccattg
    63541 agctatcccg tcagcgatta gcgtaagtta accagcgtgt tgaggatctg gtcctgggtt
    63601 ttgatggtct gggcgttaga ctgatagtta cgctgagcaa cgatcatatt gaccagttct
    63661 ttactgagat cgacgttgga cgcttccagc gcaccgttgg tcagggtgcc aaagttgccc
    63721 gtcccggctg tccccaacag cgccacgcca gaagattgcg tcgcagacca gacgttgtcg
    63781 ccttcggatg ccagaccttc gttgttggca aagttcgcca gtacaatctg ccccagcagt
    63841 tgggtttgtt cgttggaata gttgccgaca accgtaccgt catcattgat ttgataactc
    63901 accagatcgc ccggtttgta gccgttctgg gtggttgcca caatgttgtt agcgccggta
    63961 ttttgctgca tggagttgag gaagctcagg ctaaacgtgg cgggttctgc accgttaatt
    64021 gcgccagtgg aaatattcgc tgttggatct gaggtcagaa cgccattggc attaaacacc
    64081 agcgtcattg caggctcagc tgtacctgtt ggatcactgc tatcctgggt gtagacctgc
    64141 cagttattat ccccggtctt cacaaagtag acgctcatgt catgagcatt accctgacta
    64201 tcgaaaacag tcaccgaacc ttttttgtta tagctatccg cattgctagc atcaaatgcg
    64261 ttaacagagg gaagcggatc actggaattc aggttgatct gcatcgacgc cgtggtggta
    64321 gttttcgctg ccatcagggt attcggaatc gaaatattgg tcggattcgc cccttgctga
    64381 atagtcggcg gcgtaccggt tgccgggtaa cccgtcagct gtaaaccttg catattcacc
    64441 aggttacggt tttcatccag cttaaattgt ccgttacggc tgtagaacac cgaaccgttg
    64501 ctgtctacca gacggaaaaa accgttctgg ctgatagcaa cgtccagacc tcgcccggtg
    64561 ttggtggtcg tgccatcggt aaagtcctga gtgataccag caacttttac ccccagtccc
    64621 actttcgaac cggcaaacat atcggcaaaa gaggccgtgc ctgatttaaa gccgtaggtg
    64681 gcagagttgg cgatattgtt gccaataaca tcgaggttgg tggcggcagc gtttaatccg
    64741 ctaaccgctt gagaaaaggc catgactgac tcctgaaatg tgaaggctta aattatctgc
    64801 cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat ccagcgtatt accgttgttg
    64861 ccgcggatca caccctgcac cagagcaaac tgcagcggct gggcaaccag ttgtgtacca
    64921 ccgttactgg cgctaatcgc tacgttgtaa gaaccgttcg gcgcagtggt gccgtcagtc
    64981 aacgtaccgt cccaggtgaa actgtggact ccggcggtca gttcaccaat atcaatggta
    65041 cggacaaccg caccgttttt atcggtgatg gtggccgtca ctttgtctgc cgcctgttgc
    65101 agctcaacac caaacggcgt ggtcgtggtc acagcccctt cttcactgcc ggttcccgca
    65161 agaacagtgg tgccggggat catcacgccg tgaccgatca ggttactggc ctgtaacgac
    65221 tggctgttat caatctgtcc ggaaatagat ccgagcgtgg tattgagttt ttcaatcccg
    65281 ctgaccgtgc tgatttgagc caattgcgac gtcagctcgt tgttttccat tggattggtc
    65341 gggtcctggt ttttcagctg cgccaccaac aaagtcagaa aactgctttg taaatctgcg
    65401 gcgttgctgc ccgtgagcga actactactg gtggtgctga cgccggtatt tgtcggatcg
    65461 gtggtggtta ccgcaatgga catagttttc tcctttattg accgagcgta agggttttca
    65521 gcatcatgct tttcaccgtg ttgagcactt caacattggc ctgatagctg cgtgacgccg
    65581 acatggtgtt aaccatctct ccgacgacat caacgtttgg cattttcacg tagccctttg
    65641 catccgccag cggattaccc ggttcataaa ccagtttgtc cggggcctga ctttctataa
    65701 catcggcaac ctttacgccg cctgtcgcag cacctggcgc agcgttaacc tgaaatacca
    65761 cctgttttgc ccgatatggc tgtccatcgg gaccggtcac gctatcagca ttcgccagat
    65821 tactggccgc cacgttcagg cgctgggact gagcagttaa cgccgacccg gcgatatcaa
    65881 aaatattcag cagtgccatc cgttaatttc cgctctgtaa aacgttcatc atgcctttga
    65941 tttgcccgct caacgcgcta aggctcatct ggtattgcag gctgttatcg gcaaactggg
    66001 tgcgttcgcg atccatatcg acagtattac catccagcga gggctggtcc ggaatacggt
    66061 attgcagttc tgcggtagga ggcgtcagcg cctgcgccgg aatgtgttgc gttgaggtca
    66121 tcgtcagtgc aaccacgctt gttgcatccc gtccacgttg catgactttt ttaagttcac
    66181 tggcaaaatc gatatcgcgc gcctgataac caggggtatc ggcattggcg atgtttgctg
    66241 ccagcacttc ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa cgtaaggcgg
    66301 cgtcgagctt atcgagcata tctcctccgc aggtatcaaa attcggccat cagcttaaat
    66361 gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt tatgccgttg
    66421 atggtcattg cggacaggta caattcacgt tgtagaaatg gctggggggc gaaaatgctg
    66481 gcaataaaac gtagcgtggc gatcatcgcg atactgttca gtccgttaag tgcggcgagc
    66541 aatctcacat cgcaattgca cacctttttt agcgcccagc tcgcgggggt aagtgatgag
    66601 gttcgtgttt ctattcgtac agcgcccaat ctactaccgc cgtgcgagca gccattgctt
    66661 tcgatgagca ataattcccg cctgtggggc aatgtgaatg tgttggcacg ctgcggtaac
    66721 gacaaacgat atttacaggt taatgtgcag gccacaggaa attatgtggt tgccgcaatg
    66781 cccattgtgc ggggaggaaa gctggaagct ggcaatgtca aactgaaacg cggacggctg
    66841 gacaccttgc caccgcgtac ggtgctggat atcaatcaac ttgttgatgc cgttagcctg
    66901 cgcgatctat cacccgatca acctatccag ttaacccagt ttcgccaggc atggcgggta
    66961 aaagcgggac aacgcgtcaa cgtgatcgcc agcggtgatg ggtttagcgc caacgcagaa
    67021 ggtcaggcgc tgaacaatgc tgccgtcgca cagaatgcgc gggtgcgcat ggtatcggga
    67081 caagtggtca gcggcgttgt tgatgcagat gggaatattc ttataaacct gtaagctgtt
    67141 aaagattacc cgtcccttgc cgataaataa gcaacacatg ataaaagcgc cctcaatgag
    67201 gaataaacca tgagtattga tcgcacttcg cctctgaagc ccgtaagcac cgttcaaccg
    67261 cgcgaaacca ctgacgcgcc ggtaacgaac acccgggcgg caaaaacaac cgcctccacc
    67321 agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga tgcaacccgg cagcagtgat
    67381 atcaatcttg aacgtgtcga agcgttaaaa ctagcgattc gtaacggtga actaaaaatg
    67441 gacaccggca aaattgccga tgcgctgatc aacgaagcgc agcaagactt gcagagtaac
    67501 tgatcgtatg acgcgtcttg cagagatcct cgaccagatg tccgctgtgc ttaacgatct
    67561 caaaacggta atggatcaag agcagcaaca tctctctatg gggcagatca acggcagcca
    67621 gttgcaatgg attacagaac aaaaaagctc tctgctggcg acgctggatt acctcgaaca
    67681 gttacgcagg aaagaaccca atgcagcaaa tagcgttgat attagtcaac gctggcagga
    67741 aattactgtg aaaacgcagc aactacgcca aatgaatcaa cataacggct ggttactgga
    67801 aggacagatt gagcgcaatc aacaggcgct ggaaatgttg aaaccgcatc aggaaccgac
    67861 gctatatgga gcgaacggtc agacctcaac aacccatcgc ggcagtaaaa agatttcgat
    67921 ctgaagatta tctccggcct gcactgcagg ccggaatgcc tttttacacc gtccggcggg
    67981 caaactcttt aactttgaag cccagtaccg ccagtgctgc aaagtacgcg gcaatccccg
    68041 ccagcacgac cgccattaaa cgcagtaaac gccagggcat ggtacccaat gaccactccg
    68101 gcatgatatg taacatacct aaaagcacgc cagacatcac cagtaccgcc accaccagac
    68161 gcaacagaaa cgccatccag ccgggttgcg gggtaaagat tttctgctta cgcaactgcc
    68221 agtaaagcag cgaagcattc agacacgccg ccagaccaat agaaagtgac agcccggcat
    68281 gtttcaacgg accgataaac gccaggttca tcaattgcgt taaaatcagc gtaacgatgg
    68341 caattttcac tggcgtttta atgtcctggc gggaataaaa gccaggagcc aacactttca
    68401 ctacaatcag gccaatcaaa cccaccgagt aggcaattaa cgcccgctgg gtcatcagcg
    68461 catcaaaggc ggtaaattta ccgtactgga acagcgaaac ggttaacgga cctgaaagaa
    68521 tgcccaacgc aaccgcactc ggcagcgcca acaggaaaca aagacgcagc ccccagtcca
    68581 tcaaacggtt gtattcatca tgattgccac tggcaaaact tttcgacagc gacggcagca
    68641 aaatggtgcc aagcgccacg cccagtacac cggacggaaa ctccattaag cggtcggcgt
    68701 aatacatcca agacaccgaa ccggaagcaa gaaacgaggc aaaaatggtg ttgatgatta
    68761 aggagatctg gctcacagag acgccaagga tcgccggtcc catctgtttc accacgcgca
    68821 ttgctccggc atcgtggaag ttaatgcgcg gcaggaccag catgccgatc ttcttcaggt
    68881 gcggtagctg atacaccagc tgcaggatgc cgccgaccgt aacagcccac gccagcgcca
    68941 gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa accaatcatg ctgatattaa
    69001 gcagtgttgg agcaaacgcc ggaatcgaga agcggttcca cgtattcaga atcgctccca
    69061 ccagcgacgc cagggagatc agcaagatat agggaaaggt tatctttagt agctggctgg
    69121 tcagggcaaa tttgtcggca gtgtcagcga agcctggcgc ggtcaccatg atcacccacg
    69181 gtgcagcgag catgccagcg accgtcacaa ccgccagcgc gagtgtcagc aggccagaaa
    69241 cataagagac aaagacccgc gtggcgtctt caccctgctt gcttttatat tctgccagaa
    69301 tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat acggcgcaac aagttaggaa
    69361 gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc gccaaagatt ctggcgacaa
    69421 ttgcgtcacg tgcgaagcca agcacacgcg aaaacatggt catcgagctg acggcggcca
    69481 gcgattttaa taaattcatc ggtgttctaa tccagaccca cgggcaaaac gcctgcaaag
    69541 caggcgctga aagagacgaa tagtctacct ggattatggt gaattgctac cgccagatgt
    69601 tacagggttt attcactcat cgcatcgcgc cagatcttgt caacgatacg ttgcgcccgc
    69661 actgcctgtt cgccggcggt ttgcggaact gtctgatttt gcacgcattc aatgaagtgc
    69721 cgcgcacagc cgacaaatcc acgttgctca agcgtactct gccaaccagg aatcggttta
    69781 tgcactacgc cctgcccgcg ctcctcacgc cattcgcgca tatccgtaat gtcgatgagc
    69841 gcaccgtcag tcacggcctg cacggtttca cgctggcttc cggcacggcg atgcatacag
    69901 gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg caaacagcat ttcgccagcg
    69961 tcgttagtca gtagcgtacc gccatccaga gaggctttgc cgcccgacaa ccacagcgcg
    70021 gtatccacca catgcagata atcatccagc aacgtgaaat aaagatcgtg tggcccgacg
    70081 ctattgctac gatgtttatc cattcttagc gaggctgcgg tggcgagttg cgtttttaac
    70141 tctccgtaga gtggtgcgaa acgacggtta aaaccgacca tcaacgtcag ttttttccgc
    70201 gccgccagtt ccaccaaccg ttcagcatcg cgcagatttt ctgccagcgg tttatcgaca
    70261 cagacatgta cccccgcatt gagtaacgta ctgaccacgt caaagtggct ggcggtgctg
    70321 gaatgcacaa aaaccgcatc gcaactggcg gcaaggctgg ataacgaatc ggcataagga
    70381 atgcgccagc tttcacaaat tggcagggct ttcgcgcgcg taggcgacca ggctccttgt
    70441 aacatccagt cagacgctgc cgccagcacc ggtaaccacg ctttttgcgc aatgccgcct
    70501 aatcccacta cgccgatacg taattttttc actttaatct cccagatggg caagcaacga
    70561 atcaagacgc tgtttcagtt ctgccacttc gatttccagg gcttcgacgc gagcctgtaa
    70621 atcaccgtca accgcgttcg acatatccat caccgccggc tgatcttcaa cctcaccact
    70681 gaaaagatgc atgtagcggc tttcacgttt acccggttcg cgggccagac gcaccacaaa
    70741 aggaccatct tcgcgatttg ccagttgttc cagcgtcgac tccacttccg ccatatcgct
    70801 gaattcatac attcgcgcgg cgcggctgcg cagttcaccc ggcgtctggg caccacgcaa
    70861 taacaacgtg gtgattaacg ccacttctgc ggcgctcagt ttcagatcgc caaattctga
    70921 attacaaaaa cgttgctcat atttggtgac ccgattacca aaaccgctca ctgtgcgtag
    70981 ataatgacgt ttgaccagat tatccagctg ttcctgcact tcggattcac tcagattcat
    71041 gaccggttca cggttcgttt tctgattaca ggccgtgact acaccattga ctgagagcgg
    71101 gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa ccaatcacgc gagcttcaag
    71161 ggcagtaagt tgatatttca tgagattctc cttagcggcc gggcgtccag tctggggtag
    71221 ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat caacagatag tctttcgcat
    71281 agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc gctgcgttta ttgtgcggca
    71341 tataattagc cataatgcga tgaatatgtt gggtgcgctg catataacga atggctgcgg
    71401 tcagggcttc aaacatgagt cctttgccct gccatttttg cccaatcgaa taaccgagat
    71461 agcaggcatg aaaagagcca cgaacaacat tggaaaaatt ggcaacgcca ataatctctt
    71521 tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc ttgtttatga aattcgttaa
    71581 tcatccctag cctggcctgc cagcctgatg gataacagtg gctttcgtcg cgcactggct
    71641 cccagggctt gaggaaatgg cgattctctg cgtaataatc cgccagacgc caggcatcac
    71701 gatcatgcac cagacgcacg accagtcggt ctgtggttaa gcgcactttt ggcacgttac
    71761 tgcgatagcc aaacattcta tacctactcc ttcccgtaac gtctttaata cgctgtaagt
    71821 attactatac ctgcgcaacc agaccctgtg aaaagtccag aatgccattt tctgcattat
    71881 tcacatattt gataaaaact ctctatcaaa gcgacgtttt gataaaaaaa tattgtcccc
    71941 gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa agtgctcatc ttttatttcc
    72001 cgggagggga aatgtcccgc gtgtcgcagg ccaggaacct gggtaaatat ttcctgctca
    72061 tcgataatat gctggtcgtg ctggggttct ttgttgtctt cccgctgatc tctatccgct
    72121 tcgttgatca aatgggctgg gccgccgtca tggtcggtat tgccctcggc ctacgccagt
    72181 ttattcagca aggtctgggt attttcggcg gagcaattgc cgaccgcttt ggtgccaaac
    72241 cgatgattgt taccggtatg ctgatgcgtg ccgccggatt cgccacaatg ggtatcgccc
    72301 acgaaccgtg gctactgtgg ttttcatgcc tgctttcggg actcggcggc acgttgtttg
    72361 atcctccgcg ttcggcgctg gtggtgaaat taatccgtcc acagcagcgt ggtcgttttt
    72421 tctcgctgtt gatgatgcag gacagtgccg gtgcggtcat tggcgcattg ttggggagct
    72481 ggctgttgca atacgacttt cgcctggtct gcgccacagg ggcagttttg tttgtgctgt
    72541 gtgcagcgtt taatgcgtgg ttgttaccgg catggaaact ctccaccgta cgcacgcccg
    72601 ttcgcgaagg catgacccgc gtgatgcgcg acaagcgttt tgtcacctat gtactgacgc
    72661 tggcgggtta ctacatgctg gctgtacaag tgatgctgat gctgccaatt atggtcaacg
    72721 acgtggcggg cgcgccctct gccgttaaat ggatgtatgc cattgaagcg tgtctgtcgt
    72781 taacgttgct ctaccctatc gcccgctgga gtgaaaagca ttttcgtctg gaacaccggt
    72841 tgatggctgg gctattgata atgtcattaa gcatgatgcc ggtgggcatg gtcagcggtc
    72901 tgcaacaact tttcaccctg atttgtctgt tttatatcgg gtcaatcatt gccgagcctg
    72961 cgcgtgaaac cttaagtgct tcgctggcgg acgcaagagc tcgcggcagc tatatggggt
    73021 ttagccgtct gggtctggcg attggcggcg ctattggtta catcggtggc ggctggctgt
    73081 ttgacctggg caaatcggcg caccagccag agcttccgtg gatgatgctg ggcattattg
    73141 gcatcttcac tttccttgcg ctgggttggc agtttagcca gaaacgcgcc gcgcgtcgtt
    73201 tgctggaacg cgacgcctga tttgatactc ccccctgact cctccatact gaaagtgaac
    73261 tgacagccaa atggaggagt cacgtgaagc tatacattta cgatcactgc ccttactgcc
    73321 tcaaagcccg catgattttc ggcctgaaaa atatccccat cgaattacat gttctgctca
    73381 acgacgacgc agaaacaccc acccggatgg tcggtcaaaa acaggttccc attctgcaaa
    73441 aagatgacag ccgctatatg ccagaaagca tggacatcgt tcactatgtc gataaactcg
    73501 acggcaaacc gttactgacc ggcaaacgtt cccctgccat tgaagagtgg ctgcgcaagg
    73561 tcaatggcta cgccaacaaa ctgctgttgc cgcgttttgc caaatcggca tttgatgagt
    73621 tttctactcc tgccgcgcgc aaatatttcg tcgacaagaa agaggctagc gcgggtaatt
    73681 ttgccgacct gctggcccac tctgacggtc tgattaagaa tatcagcgat gatttacgtg
    73741 cgctggacaa actgatcgtc aaaccgaacg ccgtgaatgg cgaactttcg gaagatgata
    73801 ttcagctatt tccgctcctg cgtaatctga cgctggtagc cggaatcaac tggccaagcc
    73861 gcgttgctga ttaccgcgat aatatggcga aacagacaca aatcaatttg ttatcatcaa
    73921 tggcgattta atcctgaccc gccgcggcag cctgtattcg cgggttgccg ccgttttcag
    73981 ttttttctgc tggcgcgttt atcgctggtg acacatgctg cgttgtgtca ggatattgac
    74041 gcagatttca tgtcgaggac taccatgaac aagtttttat ttgccgctgc attgatcgtc
    74101 agtggcctgc tcgttggctg taatcaactc acccaataca ccatcaccga acaagaaatt
    74161 aaccagtcgc ttgcgaaaca taataatttc tcaaaagata tcggtttacc cggcgtggct
    74221 gacgcccata ttgttctgac aaacctgacc agccaaattg gtcgcgaaga gccgaataag
    74281 gttaccctaa ccggagacgc caatctggac atgaactccc tgttcggtag ccagaaagcg
    74341 accatgaaac tgaagctgaa agcgctgccc gtgtttgata aagaaaaagg cgctatcttc
    74401 ctgaaagaga tggaagtggt cgatgcgacg gtacaaccgg aaaaaatgca aacggtgatg
    74461 caaacgttgc ttccctattt aaaccaggca ttacgcaatt actttaacca gcaacctgct
    74521 tacgtcctgc gcgaagatgg cagccagggc gaagcaatgg cgaagaaact ggcgaaaggc
    74581 attgaagtga agccaggcga aattgtcatt ccgtttactg attaatcacg agggcgcatt
    74641 cgcgcccttt atttttcgtg caaaggaaaa cgtttccgct tatcctttgt gtccggcaaa
    74701 aacatccctt cagccggagc atagagatta atgactgcac catcccaggt attaaagatc
    74761 cgccgcccag acgactggca ccttcacctc cgcgatggcg acatgttaaa aactgtcgtg
    74821 ccgtatacca gcgaaattta tggacgggct atcgtaatgc ccaatctggc tccgcccgtg
    74881 accaccgttg aggctgccgt ggcttatcgc cagcgtattc ttgacgccgt acctgccggg
    74941 cacgatttca ccccgctgat gacctgttat ttgacagatt cactagaccc taatgagctt
    75001 gagcgtggat ttaacgaagg cgtgttcacc gctgccaaac tttacccggc aaacgcaacc
    75061 actaactcca gtcacggcgt gacgtcagtt gacgcaatca tgccggtact tgagcgcatg
    75121 gaaaaaatcg gtatgccgtt actggtgcat ggtgaagtga cacatgcaga tatcgacatt
    75181 tttgatcgtg aagcgcgctt tattgaaagc gtgatggaac cgctacgcca gcgtctgact
    75241 gcgctgaaag tcgtttttga gcacatcacc accaaagacg ctgccgacta tgtccgtgac
    75301 ggaaatgaac ggctggctgc caccatcact ccgcagcatc tgatgtttaa ccgcaaccat
    75361 atgctggttg gtggcgtgcg tccgcacctg tattgtctac ccatcctcaa acgcaatatc
    75421 caccaacagg cattgcgtga actggtcgcc agcggtttta atcgcgtatt cctcggtacg
    75481 gattctgcgc cgcatgcacg tcatcgcaaa gagagcagct gcggctgcgc gggctgcttc
    75541 aacgccccaa ccgcgctggg cagttacgct accgtctttg aagagatgaa tgctctgcag
    75601 cactttgaag cattctgttc tgtaaacggc ccacagttct atggcttgcc ggtcaacgac
    75661 acattcatcg aactggtccg caaagagcaa caggttgctg aaagcatcgc attgactgat
    75721 gacacgctgg tgccattcct cgccggggaa acggtacgct ggtccgttaa acaataaaaa
    75781 tcgtagcttc ctgttgtcat taggttattt taactgtata aataaccagt atattcaaca
    75841 gggggctatt atgcgaattg aagtcaccat agcgaaaact tctccattgc cagctggggc
    75901 tattgacgcc ctggctggcg aactttcccg ccgtattcag tatgcgtttc ctgataatga
    75961 aggccacgta tcggtacgtt atgccgcagc gaataattta tcggttattg gcgcgacaaa
    76021 agaagataaa cagcgcatta gcgaaattct ccaggaaacg tgggaaagcg ccgatgactg
    76081 gtttgtcagc gaataatatg cagtgatttt tgtttttgcc gggtcgcccc ggctttttta
    76141 ttttacttgc gaattgtgag tacaaaattt ctacacttcc tcaaaataat tgcacaatat
    76201 gctaaaaaaa tatgaatgac atgctgtttt attgttcata taaatcttaa aagcaaaaat
    76261 ttatctgttc ccccctttaa aaattcggtg aataccctta cttattggta tactgaagtt
    76321 gcttcagata aaaacgcatt gaacctcgaa taacgttgtc tagtaacacg aattaggggg
    76381 ccatgatgga aaaaaataat gaagtcattc agactcatcc gctcgtaggg tgggacatca
    76441 gcaccgttga tagctatgat gcgctgatgt tgcgtttgca ctaccagacc ccaaataagt
    76501 ccgagcagga agggactgaa gttggtcaga cgctctggtt aaccactgat gttgccagac
    76561 aatttatttc gatattagaa gcaggaatcg ccaaaattga atccggtgat ttcccggtaa
    76621 acgagtatcg gcgtcattaa catgacatga ccatccaccc aaaaggcacc tcaccggtgc
    76681 ctttaccatt tctgggttgt atatctgcat tcagttaatt actctgctta aaccatgaga
    76741 gcagagagaa aaaaatgaaa tacgatctca tcattattgg cagcggttcc gtaggcgctg
    76801 ccgccgggta ttatgcaacc cgtgccggtt taaacgtgct aatgaccgac gcccatatgc
    76861 caccgcatca acacggcagc caccacggcg atacgcgatt aattcgccat gcttatggtg
    76921 aaggcgaaaa gtatgtcccg ctggttctcc gcgcgcaaac gctgtgggat gaactctccc
    76981 gccacaacga agaagatccc atttttgtac gctctggcgt cattaacctt ggcccggctg
    77041 attccacatt tctcgccaac gtcgcccaca gcgccgaaca atggcaactc aacgttgaaa
    77101 agctcgatgc gcaagggatt atggcccgct ggccagaaat acgcgtcccg gacaactaca
    77161 tcggcttatt tgagactgat tccggctttt tacgcagcga actggcgatt aaaacctgga
    77221 tccaactggc gaaggaagcg ggctgtgcgc agctgttcaa ctgcccggtc accgcaattc
    77281 gtcatgacga tgatggcgta actattgaaa cggctgacgg tgagtatcag gcgaaaaaag
    77341 cgattgtctg cgcgggaaca tgggtaaaag acctgctccc ggagttgcct gtccagcccg
    77401 tacgcaaagt atttgcctgg tatcaggccg atgggcgcta tagcgtgaag aataaattcc
    77461 cggcgtttac cggtgaactt cccaatggcg atcaatatta tggttttccg gcagaaaacg
    77521 acgcgttgaa gattggcaaa cataacggag gccaggttat ccattcagcg gatgaacgtg
    77581 ttccgtttgc ggaagtggtc agcgatggtt cggaagcctt cccgttcttg cgcaatgtat
    77641 tgccgggtat cggttgctgc ctgtacggcg ccgcctgcac ctatgataat tcgcctgacg
    77701 aagattttat tatcgatacc ctacccggcc acgataatac actgctcatt accggcctga
    77761 gtgggcacgg ttttaaattt gcgtcagttt taggggaaat agctgccgat tttgcgcaag
    77821 acaaaaaaag cgattttgat ttgacgccat tcaggctttc ccgcttccaa taatcatcat
    77881 tacggcctcc agaatgaggc cgtttttaca ggagtattta atgcgtcggc tgttgcacta
    77941 tctcatcaat aatattcgcg agcatctgat gctttatctt tttctctggg gattgttggc
    78001 aattatggat ctgatctatg tgtattattt ttagttttcc gcactgacat aaactgacaa
    78061 atatttaatc tccctttcat ttagcgtgaa aaattgaatg agattattcc tgcttcaaca
    78121 gcactcacca gacatctctg atgctgaacc cactaaactt atgaagattc tttcattatt
    78181 aattcaaaat tattgaaata aaaataaata tcattcaaat tgttattttt gtgttgcaat
    78241 tcattctccg acacgccatt ttctttctct cgcaaagtcg ttttggatgc acttatgtca
    78301 ttcacaaata cccctgaacg ctatggtgtt atatctgcgg ccttccattg gctaagcgca
    78361 attattgtct acggcatgtt tgctttaggt ctgtggatgg tcacgctcag ttattacgat
    78421 ggctggtatc accaggcacc cgaactgcat aaaagcatcg gtattttgtt aatgatgggg
    78481 ctggttattc gcgttctgtg gcgcgtcata tctccaccgc cgggtccgct gcagagttat
    78541 tcgcccatga ctcgtcttgg cgcaaaggct ggacatctcg cattgtactt attgctgttc
    78601 gccatcggca tcagcggcta tctgatctca actgccgatg gtaaaccgat cagcgttttt
    78661 ggctggtttg acgtccccgc gaccctcgcc gacgctggcg cacaggctga ctttgccggt
    78721 gccctgcatt tttggctcgc gtggagcgtc gtagtactgt ccgttatgca cggatttatg
    78781 gccctgaagc atcatttcat cgataaagac gacactctga agcgcatgct gggaaagtcg
    78841 tcatctgact atggagtata aaaaatgaaa aaaagcctgc ttggtttaac cttcgcttcc
    78901 ctgatgttct ctgccggttc agcggttgcc gccgattaca aaattgacaa agaaggtcag
    78961 cacgcctttg ttaacttccg catacagcac cttggctata gctggttata cggcaccttt
    79021 aaagatttcg acggtacttt tacctttgat gaaaaaaatc cagctgccga taaagtgaat
    79081 gtgacaatta acaccaccag cgtcgatact aatcacgccg aacgcgataa acatcttcgc
    79141 agtgcagatt tcctcaatac tgcaaaatat ccacaggcaa cattcacctc caccagcgtg
    79201 aagaaagacg gtgacgaact ggatattacc ggcgatctga cgctgaatgg cgtaaccaaa
    79261 cccgtcacgc tggaagcgaa attaattggt cagggcgacg acccatgggg tggtaaacgt
    79321 gcaggcttcg aggccgaagg caaaattaag ctcaaggact tcaatatcaa gacagattta
    79381 ggtccagctt ctcaggaagt ggatctgatt atttcagtgg aaggcgtaca gcagaagtaa
    79441 gagtgaaatg ttgccggatg gcacgcatcc ggcaatgata tttattctgt tggatcagga
    79501 atgcccagtg ttgtattcag acgtccacga gacttattaa agatcttatt gccattttca
    79561 cgtcctgccc gacggcgtcg ctgttcttct ggcggtaacg cgctttcttc gcagcaaatt
    79621 tcactacaac aacctttgta tttttctgca catactggac actgaataaa cagcaggtgg
    79681 cagccatcat ttttacagtt ggtatggctg tcgcacggcg caccgcactg gtggcaatgc
    79741 gcgataatct catcagatat acgttcgccc atccgctcgt caaaaacaaa atttttgcca
    79801 ataaaacgta ccggtaaacc ctgctcgcgc gccttgcggg cgtattcaat aattccgccc
    79861 tcgatatgcc agactttatt gaatccgtta tgtttcatcc aggcactggc cttttcacaa
    79921 cgaatgccgc cggtgcagta cataacgatt tttttatctt tatgtgcctg catcatctct
    79981 actgcttttg gcagctgctc acggaaggta tctgccggaa tttccagcgc gttttcaaag
    80041 tgccccactt catactcata gtggttacgc atgtcgataa acaatgcatc gggatcgtca
    80101 agcatggcgt tcacttccgc cgcttgcaga tactcaccca cattgctggc atcaaagtga
    80161 ggatcgtcaa taccgtcggc aacgatacga tcgcggacct tcatgcgcag tacccagaag
    80221 gatttcccgt catcatccaa cgcgatattc aggcgtaagc cctctaaagc cgggtcgaag
    80281 gcataaagct gcgcgcgaaa tgtttcaaca ttgctcgcag gtacgctgat ttgcgcgtta
    80341 atgccctcat gcgccagata cactcgccca aaaacatcca gcgcggtaaa cagctgatat
    80401 aaagcgtcac gggtcgcctt aggatcggcg atgtggaaat acttgtaaaa cgaaatggtg
    80461 gttcgcggtt cgctctcagc caacattttg gcttttaacg cgtcgttgga aatgcggttg
    80521 tgtaacactg gcatggtgta cggttcctgc gagatgggaa agtaaaaatc cgcggcatga
    80581 tatagcaatt atcgataatt aacatccaca cattttacgc tacatttgcg cattaaaaat
    80641 tatttgttat ttacaagcgc ggcaatttcg cccagtcttc agccacaaat tttggttgcg
    80701 ggcgaaaaaa tgcgacaata catacaattg cccgtatagg ttgaaaaaca ggattgatat
    80761 gacgaatcta cccaagttct ccaccgcact gcttcatccg cgttattggt taacctggtt
    80821 gggtattggc gtactttggt tagtcgtgca attgccctac ccggttatct accgcctcgg
    80881 ttgtggatta ggaaaacttg cgttacgttt tatgaaacga cgcgcaaaaa ttgtgcatcg
    80941 caacctggaa ctgtgcttcc cggaaatgag cgaacaagaa cgccgtaaaa tggtggtgaa
    81001 gaatttcgaa tccgttggca tgggcctgat ggaaaccggc atggcgtggt tctggccgga
    81061 ccgcagaatc gcccgctgga cggaagtgat cggcatggaa cacattcgtg acgtgcaggc
    81121 gcaaaaacgc ggcatcctgt tagttggcat ccattttctg acactggagc ttggtgcgcg
    81181 gcagtttggt atgcaggaac cggggattgg cgtttaccgc ccgaacgata atccactgat
    81241 tgactggcta caaacctggg gccgtttgcg ctcaaataaa tcgatgctcg accgcaaaga
    81301 tttaaaaggc atgattaaaa ccctgaaaaa aggcgaagtg gtctggtacg caccggatca
    81361 tgattacggc ccgcgctcaa gcgtttttgt cccgttgttt gccgttgagc aggctgcgac
    81421 cacgaccgga acctggatgc tggcacggat gtccggcgca tgtctggtgc ccttcgttcc
    81481 acgccgtaag ccagatggca aagggtatca gttgattatg ctgccgccag agtgttctcc
    81541 gccgctggat gatgccgaaa ctaccgccgc gtggatgaac aaagtggtcg aaaaatgcat
    81601 catgatggca ccagagcagt atatgtggtt acaccgtcgc tttaaaacac gtccggaagg
    81661 cgttccttca cgctattaaa tctcccaagc cggatgcttg agaatagcat ccggcattac
    81721 cacagcaaat ccccctgatt tagcgataaa agctctctgg attgcgcccc ctggaagtcg
    81781 ggcgcataat tagtgtgctt atcttttctt cttatgttca ccgcgcctgg cgcaccaaca
    81841 gcggattgct atgtcaccct gtgaaaatga cacccctata aactggaaac gaaacctgat
    81901 cgtcgcctgg ctaggctgtt ttcttacggg tgccgccttc agcctggtaa tgcccttctt
    81961 acccctctac gttgagcagc ttggcgttac gggccactcc gccttgaata tgtggtccgg
    82021 tattgtcttc agcattacat ttttattttc ggccatcgcc tcaccgtttt ggggtggact
    82081 cgccgaccgt aaaggccgaa aactcatgct attacgctct gccctcggca tgggcatcgt
    82141 gatggtgttg atgggactgg cacaaaatat ctggcagttt ttgatcctgc gggcgcttct
    82201 tgggttactt ggcggatttg tccccaacgc taatgctctt atcgccacac aagtaccgcg
    82261 taataaaagc ggctgggcgc tgggtacgct ctccacaggc ggcgttagtg gtgcgttgct
    82321 cggcccaatg gctggcggcc tgctcgccga tagctacggc ttacgtccgg tattctttat
    82381 taccgccagt gtgctcatac tctgcttttt cgtcaccctg ttctgcatca gagaaaaatt
    82441 ccagccggtc agcaaaaaag agatgctgca catgcgggaa gtggtgacat cacttaaaaa
    82501 cccgaaactg gtactcagcc tgtttgtcac cacgttaatc atccaggtgg cgacaggctc
    82561 aattgccccc attctgacgc tgtatgtccg cgaactggcg ggtaacgtca gtaacgtcgc
    82621 ctttatcagt ggcatgatcg cctcggtgcc aggcgtggcg gctctgctaa gcgcaccacg
    82681 actcggcaaa cttggcgatc gaatcggacc cgaaaagatc ctgattacag cgctgatctt
    82741 ttctgtactg ctgttgatcc caatgtctta cgttcagacg ccattgcaac ttgggatttt
    82801 acgttttttg ctcggtgccg ccgatggtgc actactcccc gccgtacaga cactgttggt
    82861 ttacaactcg agcaaccaga tcgccgggcg tatcttcagc tataaccaat cgtttcgtga
    82921 tattggcaac gttaccggac cattgatggg agcagcgatt tcagcgaact acggtttcag
    82981 agcggtattt ctcgtcaccg ctggcgtcgt gttattcaac gcagtctatt catggaacag
    83041 tctacgtcgt cgtcgaatac cccaggtatc gaactgattt ttcgcctttc atacttgcaa
    83101 aagcggagaa tcagctatcc ttttccctga aacctcatca actcaaaggg agaatcgtga
    83161 tgaccatgta cgcaacgctt gaagaagcca ttgacgctgc acgcgaagaa tttcttgcag
    83221 acaaccccgg catcgacgcc gaagatgcga atgtgcaaca gttcaatgcc caaaaatacg
    83281 ttttgcagga cggcgacatc atgtggcaag ttgagttttt tgccgacgaa ggggaagaag
    83341 gtgaatgttt acctatgctt agcggtgaag ccgcgcaaag tgtttttgat ggcgactatg
    83401 atgagataga gatacgccag gagtggcagg aagagaatac attacatgaa tgggacgagg
    83461 gggaatttca gcttgagcca ccgctggata ccgaggaagg acgcgcagca gctgatgagt
    83521 gggatgaacg ttaatcactc atacgggcca tgatgaatgt cgatcggcag cagtaaagta
    83581 tcgaaaacca gggagaatgg cagatcaagg atcgtgacgt aacgccaggc ggagtcacgc
    83641 acgtcccatt gcacaccggg ataatattgg ttgccatgcc cctgccccgg tatagtgcga
    83701 ctaatgatgc tgccacagcc actcagcaag gtcaccatta tgctcatcac aattaaacgc
    83761 atttgatacc tcttccgtaa aaaataccgg cataacgccg gtatttttat tggctcgttg
    83821 tttttgccgg atgcggcgtg aacgctttac ccgacctaca aaatcttgca aaatcaataa
    83881 attgcaggaa cgatgtaggc ctgataagcg tagcgcatca ggcaactacg tttttattgc
    83941 gaagccgcat ccggtttacg caatgccagt ggattgagct ttatcccttc gtaataactc
    84001 acccatgaag aatatctctc cggggaattc cagacgcgga aatgcagacg ggccatcgtc
    84061 accggatcgc ttagcagcac caggcgacga tcgcgattca gcttctctgg cgtctcgttc
    84121 agcgcctgtt caacgtggcg gtcacgggcg atttccagca ccttgctggc gcggtgacgc
    84181 gcggtcgcca ttgcggttgc cagagcgtta aatgacgggt taaacaccgc gtgcataaaa
    84241 ccatcatcaa gggagcgttg acgattcatc tcaaggaacc gatcggtatc aaccagcacc
    84301 tgcggcggtg aatactcttc cgggatcagg aacagtttcc agcgtttggt tcgcagacca
    84361 acggtggcac ggctggaaat caccgaaaca aacggtgaca ggatcaacga gaagacaatc
    84421 ggtgccagcc agaacagaaa acgcagatcc aaccacgcca tcccaacagc ccacactaaa
    84481 cccaacagca gctgtgagcc gtggcgtttg aatgcttcgc cccaggaagt ggagtcatca
    84541 tcacgctgcg gtgaattcca caccacttcc cagccaagga acgcgctgac gacgaagact
    84601 gtatggaaca acatgcgtac cggagccagc aacacggaaa acagcacttc cagcagcaac
    84661 gataatgtaa cgcgccagaa gccgccgtat tctttcgttc ctttgcacca gataagcaaa
    84721 atgctcaata gcttcggcag gaacaacagc accatggtcg aagcaaaaag tgcaatcgcc
    84781 agctcaggac gccactgcgg ccataccggg aacaactgcc gtggttgcag gaagtattgc
    84841 ggttcggtca acgcatgtac tacctgcaat gcagtagaga gcgcgaggaa cataaaccac
    84901 agcggagcgg agagataaga catcacgccc gtcaggaaca ccgcacggtg aaccgggtgc
    84961 atacccttca ccaggaacag acggaagttc atcaggttac cgtggcacca gcggcggtca
    85021 cgttttagct catcaagcaa gttaggcggc aattcttcat aagaacccgg gagatcgtaa
    85081 gcaatccaca ccccccaacc tgcacggcgc atcaacgccg cttctacgaa gtcatgtgac
    85141 aggattgaac cggcaaaaga accttcgccc ggcagcggag ccagtgcaca atgctcgata
    85201 aacggtttca cgcggataat cgcgttatgc ccccagtagt gcgactcgcc aagttgccag
    85261 aagtgcaaac cggctgtaaa cagtggccca tacacgcggg tcgcgaactg ctgacagcgc
    85321 gcatacagcg tatccatacc ggacgctttc ggcgacgact gaatgatccc ggcgttcggg
    85381 ttggcttcca tcaagcgcac caggccgcat aaacaatcac cggtcattac cgagtcagca
    85441 tccagcacca ccatgtagct gtactggctg ccccagcgac ggcagaagtc atcgatatta
    85501 ccgcttttac gcttcacgcg acggcggcgg cggcgataga aaatctgacc ttcgccaccg
    85561 acttcagcga taagctccat ccaggctttt tgctctgcga cgcagatatc cgggttataa
    85621 ctgtcactca gaatgtagac atcaaagtgt ttggcattcc cggtggcttt tactgattcc
    85681 cacgttgcac gcaagccagc aaaaacacgg ttcacgtctt cgttacagat aggcatgatc
    85741 aacgccgtgc gatgctccgg gtttaatggt tcatcgccaa ctgttgacgc ggatatactg
    85801 tatttatcgc gaccaataag cagttgcagg aagcccatca acgccgtcca gaatccggcg
    85861 gacacccaac agaacagtac cgcaaagagg atcaggatac cggtttgcag catataaggc
    85921 agaagctgca taaaagaaac ccacacatcc tgaccaacca tatccatagg attaatcagc
    85981 gcccacccct gataaggaag aatggtcttc atataccagg tcgcgacaac agtttgcgcg
    86041 agcgtcagga tcaacagaat gtaacggcgg atggtaccga cggtacgcca cttttgctcg
    86101 ctctcctgct cttctttggt caaacgagcc agatagcgcg gcgtcacatc gcgtccacgc
    86161 aggcgatccc agaaacggcc taccgggttg gtacgccacg ggtcgggaaa catcgaggag
    86221 cgttttgctt ctggcatcgc cttaagctga tcgcgccctt cgtcatcttt aattaactgt
    86281 ccatcagcaa gtgaatctgg ccaggcttgt tccagacgcg cctttaccga gccttgcggg
    86341 gagtcatcct cccgcgccca ggtgcggtgt tcggcatcca gcgcctgatg aacggcgcgg
    86401 atatcagtct tcggcaatgc cgctttctcg cttgcggcga tgggcattgc gtcaatgtac
    86461 tcagttgtct tattcattgg caggtaactg gtagctccag gtttcactca acgtctgatc
    86521 ggcattcacc agcgcagcac gcatttcagt ggttttcttg gcatctttca ctttcacacg
    86581 catcaccaga cgccagcctt tggtaaccgg gttatagcgc accgtgcttt caactatctc
    86641 accattatca ccaatgctgg tttgcgctgt gaccggggta tcctctggca gttttttcat
    86701 ctctgcgccg gtaaaatcga ccacaaaggc gatagtaccg tcaggttggc gaatcaggtt
    86761 cgactgcttc acatcccccg ttgaacgacg cgtttgttgc acccatgcgt tatctggcgc
    86821 atgcagtttg tcttcatcac ggctgaaggt gatggtgtat ttaaagttca tctctttacc
    86881 cggctccggc agctgatccg gcgtccagta agcgacgatg ttatcgttgg tttcatcgtt
    86941 ggttggaatt tccaccagct caacgctgcc tttgccccac tcccctttcg gagtcaccca
    87001 tgcgcttgga cggagatcgt aacgatcatc gagatcttca aagcgggaga aatcacgacc
    87061 gcgctgcaac agaccgaagc cttgcgggtt ttccatcgag aagctgctga ccgctaaatg
    87121 tttcgggtta ttcaacggac gccagatcca ttcgccatta ccagcatgga tagagagacc
    87181 gttagagtcg tgcaactccg gacgatagtt atttgcaggc gacggttggt tcggcccaaa
    87241 caggaacata ctggttaacg gtgcaacccc cagtttgccg actttatcgc gcagatagat
    87301 tttcgactgc acatccacaa ccgtgtcacg ccctggcata actacgaatt tgtaagcacc
    87361 tgtcgcgcgc ggcgagtcaa gcaatgcata aatggttaaa cgtttatcag tcggttttgg
    87421 acgctcgatc cagaactctt tgaagcgtgg aaattcttca cccgatggca aggcggtatc
    87481 aattgccagg ccgcgtgcag aaaggccata aacctgacct gcaccaatca cgcggaaata
    87541 gctggccccg agcatgctga cgatttcatc gtttttatct ttgctgttga tcgggtaaag
    87601 cactttaaaa ccggcaaaac caaggtcttt taccgtgtct ttgtcatgct gaacatcgcc
    87661 gaaagtgaaa taatccgggc tgtatttgat tcgtttgact gcggtggcag tcacttcatt
    87721 tattttgacc ggggtatcga agtacatacc ctgatggtag aactcgagtt tgaatggggt
    87781 cttcagattg ttccagtacg ctttgtcatg attaaactgg atctgctgat agtccgcgta
    87841 tttcatgtcg cggaaaacgg agggcaagtt gcttttaggc gcctcatagc ctttcccggc
    87901 taaggattga gcttgctttg cgacatcatc aatactgaaa gcccagcttg aagatgtata
    87961 cagggttaac attactgcag cactcaacca acgcattttc atcatttgta gtttatgttt
    88021 cataattagt aagcacttcc ccctttgtgt gcttatatcg atccgatcta ttttaatgga
    88081 taaccagcca ttccgacaac agaatcccgg tatcgttcag cacgctggct catggattaa
    88141 gcacctgatt gcaaccgttt tcactttccg tgcttctcct aaagacagaa gcatcgactt
    88201 cgtgtagggt ttgccagcaa atttatttat caaccgtctt acagacgctt ccattctggt
    88261 tagtggtagc gatgatgcta tgcataaaaa ccttgaagcc tgcgcctggc gcggtcaggt
    88321 cggtgtcgct atggcaacaa gcctaaaggc ataacccgac agggataaga cgaaaaatcc
    88381 gagattagtt aaccatatat gaatccagta cccgcgcaac gtgaatattt cctcgactcc
    88441 atccgcgcct ggctgatgtt gttagggatc ccttttcata tttctttaat ctattcgagc
    88501 catacatggc atgtgaatag cgccgaaccg tcattgtggc tgaccctttt taatgacttc
    88561 atccactcgt tccgcatgca ggtatttttc gttatatccg ggtacttttc ctacatgctt
    88621 tttttacgct atcccttgaa aaaatggtgg aaagtacgtg tcgaacgtgt aggtatcccg
    88681 atgttaacag ccatccccct actgacatta ccgcaattta ttatgctgca atacgtcaaa
    88741 gggaaagcgg aaagttggcc tgggctatca ttgtatgaca aatataatac gttggcctgg
    88801 gaattaatat cacacctgtg gtttttactg gtgttagtag tcatgacgac gctgtgcgta
    88861 tggatattta aacgcatcag aaataattta gaaaattctg ataaaacgaa taaaaaattc
    88921 tcgatggtaa aactatcggt gatttttttg tgcctcggca tcggttatgc ggtaataaga
    88981 agaacgattt ttattgtgta tccgcccatt ctgagtaatg gcatgttcaa ttttattgtc
    89041 atgcaaacgc tattttattt gccgttcttt atcctcggcg cactggcttt cattttccct
    89101 catcttaaag ccttgtttac cacgccgtct cgtggctgta cccttgcagc agcattggca
    89161 tttgtcgctt atttactcaa ccagcgctat ggcagtggcg atgcctggat gtacgaaacc
    89221 gagtcggtga tcaccatggt cctcggtctg tggatggtga atgtggtctt ctcctttggc
    89281 caccgtttgc ttaacttcca gtcagcgcgg gtgacttatt ttgttaacgc atcgctgttt
    89341 atctatctgg ttcaccaccc gttaacgctg tttttcggcg catacattac accgcacatc
    89401 acctccaact ggcttggttt tctctgtggc ctgatattcg tagtagggat tgcgataatt
    89461 ctgtatgaaa ttcatttacg catcccgtta ctgaagtttt tgttctctgg taaaccggtt
    89521 gtcaagcgtg agaacgataa agcaccagcc cgttaagcca cgcttataat aaccattcca
    89581 cgggcaatat cgacgccagt ctgaccataa cccgcttcca gaaactggtg gcgggttctt
    89641 ttttgagaat aatctctttc tttgcatgac gatcaaccca gttgatccgt ccccacctgt
    89701 ccagacggag ctgccaggcc gcatcatact ggctctgaat aaagcgttta tcaattaact
    89761 gtgccagcgt ttcgctctct atcacgaagc ccatttcagt attgagcaat gttgaacgcg
    89821 gatcgaaatt gaaagagccg ataaacaccg ttttaccatc gatgctaaag gttttagcat
    89881 gcaagctggc accggaatta ccggttatgc cgcgatcgtg taacgtacta ctttgttcac
    89941 gcgtcggctt gagttcatat aattccacgc catagcggag caattttttg cgccagcgcg
    90001 cgtatccggc atggacgaca gcaacatcgt tagcggcaag agaattggtt aggatcgcaa
    90061 tctttacccc ttttctcacc atccgtaaga gttgcgccac acctgcgcgt gtcggtacaa
    90121 aataggaaga gataatatcg atgcgttcac tgggtgagcc catgatatcg aacaggcgct
    90181 gcggtaacag tgaatgccgt tttgccttgc cttccccttt cgccggatca tcacttaata
    90241 aacgtgtttt cgcccagata agcggcaacg ttccatcaac cagatgattt ataaacgggc
    90301 tggattccat tttgcgtaaa taacgatgcg tcatggcatc gttgtgccag gatgctggta
    90361 actcgatgcg atccgccatt tcaccttccg ggacatccag cacctgctgt aggggtgaaa
    90421 ccgatttgca ataccagtag cgggcgaaat catcggcaac gtcctctaca actggtccta
    90481 ttgccatgac atctaaatcc gaaaaaagtg gctcttcccc tgccccaaaa taggcatcac
    90541 caatatttcg cccccctacc agcgtcacca cgccatcgac agtgaaactt ttattgtgca
    90601 tacggcgatt gagacgggaa aagtcggtga tataaccaag cggacgtagc aagcgaaacg
    90661 agaaaggatt aaaaagccgg acttcaatgc ggggatgact gtcaagcaag cgtaaaatgt
    90721 cgtcaagtcc gggcgtattg ttgtcgtcca gcaacaaacg gacgcgaacg ccacgctttg
    90781 ctgcggctaa cagggcggaa aagagtaacc gacccgacat atcgtcctgc cagatgtaat
    90841 actgaacatc cagcgtatgc tctgccattt cggccagacg ataccgggcg gcaaacgcat
    90901 ccagactttt ctccagagga aaaatgccac actgaccggg atgttgcgaa cacagtggca
    90961 gcaccgcact cgccagccgg ggcaaatcat tcatctcctt gttgagtaag gagtctttcg
    91021 tagaggtggg cgttttcttc atcataacag acaaagtata cctgttcggg taaagcgtga
    91081 cgggtaataa attctgaaac ggtttttact gcgatttcag ccgctgccgc acgagggtaa
    91141 ccataaactc cagtactgat tgcaggaaaa gccactgacg tatagctgtt tgctgccacc
    91201 agtcgcaggc tattgagata ggcatcctgc aaaagctggt cttcgttttg ttcaccacca
    91261 cgccagactg gcccgacggt gtgcactacg gctttcgcgg gaagatcgcc tgcaagcgta
    91321 ataacggcat gccccgtagg gcaatcgccc tgctgctgcc tgacttttaa acaggcatcc
    91381 agcagggccg gacccgctgc gcgatgaatg gccccatcga cgccgccgcc tcccattaat
    91441 gacggattag ccgcattcac aatcacatca acggccagtt tggtaatatc accctgcaca
    91501 acatgaatac gcgttttcat aacgcctcct tacaccttct tgtttcttaa gtgtacagca
    91561 ggctttttgg ttaaatcgca tttttctgct cagcaacgtg agttgttcag aaacttagtt
    91621 atattcgatg ttcattacag ccagcttttt ctgtttatca agatactgca cttttactga
    91681 ggctatttgc gcgtttttga cattgccgct cattaacgct ttgctggaat aggttctgcc
    91741 cggaacagag ccattctgtg ggcagttcat ttctacgggt gatgactggg tgctgacgtc
    91801 acatgccggt tccacaattt ggccataaaa atggatcacg cccccttgcg cggtgcttcc
    91861 tgcgatggca ataaaaggaa aaaacatact gccgagcatt agagagtcta aaaaaggtct
    91921 gaacatttat tctatttcct caatgattag tcatccttga gggttgtgtt atccatactt
    91981 tcaggttggc tgatccgcag cagaccattc tctccagatt catcttatgc tcgatatttc
    92041 aacaaattaa gacttttctg aagagggcgg ccattgttgt gataaatgaa gtgactgtcc
    92101 atcagaaaca gtaacaacta ttttcacccg atcgtccggg gaaatattta aactcaactt
    92161 cgtcaaagca atgggttgat tagcaggcaa tgagagggtc ttttcttgct tcgtctgact
    92221 ttgccctgaa ctgccttcgc gcagggacaa tatttgtact ctgcacagac aagattgagt
    92281 aagagtgact tcaggaataa tggtgtacat atccccttgc tgggtcgtat taaaggttat
    92341 ctgactggaa agtgccgcaa ggagtaataa cgcattcata ttcttctcc
//
LOCUS       DAZKGF010000002        77897 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZKGF010000002 DAZKGF010000000
VERSION     DAZKGF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.002"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..478
                     /gene="agaB"
                     /locus_tag="ACHOQV_000095"
     CDS             2..478
                     /gene="agaB"
                     /locus_tag="ACHOQV_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFP9628530.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMSITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            517..1320
                     /gene="agaC"
                     /locus_tag="ACHOQV_000096"
     CDS             517..1320
                     /gene="agaC"
                     /locus_tag="ACHOQV_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFP9628531.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1310..2101
                     /gene="agaD"
                     /locus_tag="ACHOQV_000097"
     CDS             1310..2101
                     /gene="agaD"
                     /locus_tag="ACHOQV_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFP9628532.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPLLKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHIGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2102..2869
                     /gene="agaI"
                     /locus_tag="ACHOQV_000098"
     CDS             2102..2869
                     /gene="agaI"
                     /locus_tag="ACHOQV_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFP9628533.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNYFTCMIDEMCRR"
     gene            complement(2919..3782)
                     /gene="rsmI"
                     /locus_tag="ACHOQV_000099"
     CDS             complement(2919..3782)
                     /gene="rsmI"
                     /locus_tag="ACHOQV_000099"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP9628534.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            3847..5883
                     /gene="lpoA"
                     /locus_tag="ACHOQV_000100"
     CDS             3847..5883
                     /gene="lpoA"
                     /locus_tag="ACHOQV_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFP9628535.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSELTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            5841..6236
                     /gene="yraN"
                     /locus_tag="ACHOQV_000101"
     CDS             5841..6236
                     /gene="yraN"
                     /locus_tag="ACHOQV_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFP9628536.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDVWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRITVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            6256..6846
                     /gene="diaA"
                     /locus_tag="ACHOQV_000102"
     CDS             6256..6846
                     /gene="diaA"
                     /locus_tag="ACHOQV_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFP9628537.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            6856..7431
                     /gene="dolP"
                     /locus_tag="ACHOQV_000103"
     CDS             6856..7431
                     /gene="dolP"
                     /locus_tag="ACHOQV_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP9628538.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(7544..8584)
                     /gene="yraQ"
                     /locus_tag="ACHOQV_000104"
     CDS             complement(7544..8584)
                     /gene="yraQ"
                     /locus_tag="ACHOQV_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9628539.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVLLGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(8657..9292)
                     /gene="yraR"
                     /locus_tag="ACHOQV_000105"
     CDS             complement(8657..9292)
                     /gene="yraR"
                     /locus_tag="ACHOQV_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP9628540.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            9420..9938
                     /gene="yhbO"
                     /locus_tag="ACHOQV_000106"
     CDS             9420..9938
                     /gene="yhbO"
                     /locus_tag="ACHOQV_000106"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFP9628541.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVAIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(9918..10361)
                     /gene="yhbP"
                     /locus_tag="ACHOQV_000107"
     CDS             complement(9918..10361)
                     /gene="yhbP"
                     /locus_tag="ACHOQV_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFP9628542.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            10412..10714
                     /gene="yhbQ"
                     /locus_tag="ACHOQV_000108"
     CDS             10412..10714
                     /gene="yhbQ"
                     /locus_tag="ACHOQV_000108"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFP9628543.1"
                     /translation="MTPWFLYLIRTADDKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(10701..11204)
                     /gene="yhbS"
                     /locus_tag="ACHOQV_000109"
     CDS             complement(10701..11204)
                     /gene="yhbS"
                     /locus_tag="ACHOQV_000109"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9628544.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(11198..11722)
                     /gene="ubiT"
                     /locus_tag="ACHOQV_000110"
     CDS             complement(11198..11722)
                     /gene="ubiT"
                     /locus_tag="ACHOQV_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFP9628545.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            11931..12926
                     /gene="ubiU"
                     /locus_tag="ACHOQV_000111"
     CDS             11931..12926
                     /gene="ubiU"
                     /locus_tag="ACHOQV_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP9628546.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            12935..13813
                     /gene="ubiV"
                     /locus_tag="ACHOQV_000112"
     CDS             12935..13813
                     /gene="ubiV"
                     /locus_tag="ACHOQV_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP9628547.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAILDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            13894..14901
                     /gene="yhbW"
                     /locus_tag="ACHOQV_000113"
     CDS             13894..14901
                     /gene="yhbW"
                     /locus_tag="ACHOQV_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFP9628548.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(15019..16263)
                     /gene="mtr"
                     /locus_tag="ACHOQV_000114"
     CDS             complement(15019..16263)
                     /gene="mtr"
                     /locus_tag="ACHOQV_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFP9628549.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPTTLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(16417..18306)
                     /gene="deaD"
                     /locus_tag="ACHOQV_000115"
     CDS             complement(16417..18306)
                     /gene="deaD"
                     /locus_tag="ACHOQV_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFP9628550.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(18299..18379)
                     /gene="yrbN"
                     /locus_tag="ACHOQV_000116"
     CDS             complement(18299..18379)
                     /gene="yrbN"
                     /locus_tag="ACHOQV_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFP9628551.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(18486..19370)
                     /gene="nlpI"
                     /locus_tag="ACHOQV_000117"
     CDS             complement(18486..19370)
                     /gene="nlpI"
                     /locus_tag="ACHOQV_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFP9628552.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(19479..21614)
                     /gene="pnp"
                     /locus_tag="ACHOQV_000118"
     CDS             complement(19479..21614)
                     /gene="pnp"
                     /locus_tag="ACHOQV_000118"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP9628553.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(21861..22130)
                     /gene="rpsO"
                     /locus_tag="ACHOQV_000119"
     CDS             complement(21861..22130)
                     /gene="rpsO"
                     /locus_tag="ACHOQV_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP9628554.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(22279..23223)
                     /gene="truB"
                     /locus_tag="ACHOQV_000120"
     CDS             complement(22279..23223)
                     /gene="truB"
                     /locus_tag="ACHOQV_000120"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP9628555.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(23223..23624)
                     /gene="rbfA"
                     /locus_tag="ACHOQV_000121"
     CDS             complement(23223..23624)
                     /gene="rbfA"
                     /locus_tag="ACHOQV_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP9628556.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(23788..26460)
                     /gene="infB"
                     /locus_tag="ACHOQV_000122"
     CDS             complement(23788..26460)
                     /gene="infB"
                     /locus_tag="ACHOQV_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP9628557.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(26485..27972)
                     /gene="nusA"
                     /locus_tag="ACHOQV_000123"
     CDS             complement(26485..27972)
                     /gene="nusA"
                     /locus_tag="ACHOQV_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP9628558.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(28000..28452)
                     /gene="rimP"
                     /locus_tag="ACHOQV_000124"
     CDS             complement(28000..28452)
                     /gene="rimP"
                     /locus_tag="ACHOQV_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP9628559.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(28659..28735)
                     /locus_tag="ACHOQV_000125"
     tRNA            complement(28659..28735)
                     /locus_tag="ACHOQV_000125"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28699..28701),aa:Met,seq:cat)
     gene            29083..30426
                     /gene="argG"
                     /locus_tag="ACHOQV_000126"
     CDS             29083..30426
                     /gene="argG"
                     /locus_tag="ACHOQV_000126"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFP9628560.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(30434..32059)
                     /gene="yhbX"
                     /locus_tag="ACHOQV_000127"
     CDS             complement(30434..32059)
                     /gene="yhbX"
                     /locus_tag="ACHOQV_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9628561.1"
                     /translation="MTVFNKFVRSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(32516..32602)
                     /locus_tag="ACHOQV_000128"
     tRNA            complement(32516..32602)
                     /locus_tag="ACHOQV_000128"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32566..32568),aa:Leu,seq:gag)
     gene            complement(32617..32949)
                     /gene="secG"
                     /locus_tag="ACHOQV_000129"
     CDS             complement(32617..32949)
                     /gene="secG"
                     /locus_tag="ACHOQV_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP9628562.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQIQPAAPA
                     KPTSDIPN"
     gene            complement(33177..34514)
                     /gene="glmM"
                     /locus_tag="ACHOQV_000130"
     CDS             complement(33177..34514)
                     /gene="glmM"
                     /locus_tag="ACHOQV_000130"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP9628563.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(34507..35355)
                     /gene="folP"
                     /locus_tag="ACHOQV_000131"
     CDS             complement(34507..35355)
                     /gene="folP"
                     /locus_tag="ACHOQV_000131"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFP9628564.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYHDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(35445..37379)
                     /gene="ftsH"
                     /locus_tag="ACHOQV_000132"
     CDS             complement(35445..37379)
                     /gene="ftsH"
                     /locus_tag="ACHOQV_000132"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP9628565.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(37479..38108)
                     /gene="rlmE"
                     /locus_tag="ACHOQV_000133"
     CDS             complement(37479..38108)
                     /gene="rlmE"
                     /locus_tag="ACHOQV_000133"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFP9628566.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            38234..38527
                     /gene="yhbY"
                     /locus_tag="ACHOQV_000134"
     CDS             38234..38527
                     /gene="yhbY"
                     /locus_tag="ACHOQV_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP9628567.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(38683..39159)
                     /gene="greA"
                     /locus_tag="ACHOQV_000135"
     CDS             complement(38683..39159)
                     /gene="greA"
                     /locus_tag="ACHOQV_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP9628568.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            39407..40840
                     /gene="dacB"
                     /locus_tag="ACHOQV_000136"
     CDS             39407..40840
                     /gene="dacB"
                     /locus_tag="ACHOQV_000136"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP9628569.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLRRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40881..42053)
                     /gene="cgtA"
                     /locus_tag="ACHOQV_000137"
     CDS             complement(40881..42053)
                     /gene="cgtA"
                     /locus_tag="ACHOQV_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFP9628570.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(42069..43034)
                     /gene="yhbE"
                     /locus_tag="ACHOQV_000138"
     CDS             complement(42069..43034)
                     /gene="yhbE"
                     /locus_tag="ACHOQV_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9628571.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLGPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(43161..43418)
                     /gene="rpmA"
                     /locus_tag="ACHOQV_000139"
     CDS             complement(43161..43418)
                     /gene="rpmA"
                     /locus_tag="ACHOQV_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP9628572.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(43439..43750)
                     /gene="rplU"
                     /locus_tag="ACHOQV_000140"
     CDS             complement(43439..43750)
                     /gene="rplU"
                     /locus_tag="ACHOQV_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP9628573.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            44009..44980
                     /gene="ispB"
                     /locus_tag="ACHOQV_000141"
     CDS             44009..44980
                     /gene="ispB"
                     /locus_tag="ACHOQV_000141"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFP9628574.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            45208..45486
                     /gene="sfsB"
                     /locus_tag="ACHOQV_000142"
     CDS             45208..45486
                     /gene="sfsB"
                     /locus_tag="ACHOQV_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFP9628575.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(45534..46793)
                     /gene="murA"
                     /locus_tag="ACHOQV_000143"
     CDS             complement(45534..46793)
                     /gene="murA"
                     /locus_tag="ACHOQV_000143"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9628576.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(46848..47102)
                     /gene="ibaG"
                     /locus_tag="ACHOQV_000144"
     CDS             complement(46848..47102)
                     /gene="ibaG"
                     /locus_tag="ACHOQV_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFP9628577.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(47262..47555)
                     /gene="mlaB"
                     /locus_tag="ACHOQV_000145"
     CDS             complement(47262..47555)
                     /gene="mlaB"
                     /locus_tag="ACHOQV_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFP9628578.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(47555..48190)
                     /gene="mlaC"
                     /locus_tag="ACHOQV_000146"
     CDS             complement(47555..48190)
                     /gene="mlaC"
                     /locus_tag="ACHOQV_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFP9628579.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(48209..48760)
                     /gene="mlaD"
                     /locus_tag="ACHOQV_000147"
     CDS             complement(48209..48760)
                     /gene="mlaD"
                     /locus_tag="ACHOQV_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFP9628580.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(48765..49547)
                     /gene="mlaE"
                     /locus_tag="ACHOQV_000148"
     CDS             complement(48765..49547)
                     /gene="mlaE"
                     /locus_tag="ACHOQV_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFP9628581.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(49555..50364)
                     /gene="mlaF"
                     /locus_tag="ACHOQV_000149"
     CDS             complement(49555..50364)
                     /gene="mlaF"
                     /locus_tag="ACHOQV_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFP9628582.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            50574..51551
                     /gene="yrbG"
                     /locus_tag="ACHOQV_000150"
     CDS             50574..51551
                     /gene="yrbG"
                     /locus_tag="ACHOQV_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFP9628583.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            51565..52551
                     /gene="kdsD"
                     /locus_tag="ACHOQV_000151"
     CDS             51565..52551
                     /gene="kdsD"
                     /locus_tag="ACHOQV_000151"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFP9628584.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGVTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            52572..53138
                     /gene="kdsC"
                     /locus_tag="ACHOQV_000152"
     CDS             52572..53138
                     /gene="kdsC"
                     /locus_tag="ACHOQV_000152"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFP9628585.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSIAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            53135..53710
                     /gene="lptC"
                     /locus_tag="ACHOQV_000153"
     CDS             53135..53710
                     /gene="lptC"
                     /locus_tag="ACHOQV_000153"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFP9628586.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            53679..54236
                     /gene="lptA"
                     /locus_tag="ACHOQV_000154"
     CDS             53679..54236
                     /gene="lptA"
                     /locus_tag="ACHOQV_000154"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFP9628587.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            54243..54968
                     /gene="lptB"
                     /locus_tag="ACHOQV_000155"
     CDS             54243..54968
                     /gene="lptB"
                     /locus_tag="ACHOQV_000155"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFP9628588.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            55016..56449
                     /gene="rpoN"
                     /locus_tag="ACHOQV_000156"
     CDS             55016..56449
                     /gene="rpoN"
                     /locus_tag="ACHOQV_000156"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP9628589.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            56472..56759
                     /gene="hpf"
                     /locus_tag="ACHOQV_000157"
     CDS             56472..56759
                     /gene="hpf"
                     /locus_tag="ACHOQV_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFP9628590.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56877..57368
                     /gene="ptsN"
                     /locus_tag="ACHOQV_000158"
     CDS             56877..57368
                     /gene="ptsN"
                     /locus_tag="ACHOQV_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFP9628591.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            57414..58268
                     /gene="rapZ"
                     /locus_tag="ACHOQV_000159"
     CDS             57414..58268
                     /gene="rapZ"
                     /locus_tag="ACHOQV_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP9628592.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            58265..58537
                     /gene="npr"
                     /locus_tag="ACHOQV_000160"
     CDS             58265..58537
                     /gene="npr"
                     /locus_tag="ACHOQV_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFP9628593.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            58750..59382
                     /gene="yrbL"
                     /locus_tag="ACHOQV_000161"
     CDS             58750..59382
                     /gene="yrbL"
                     /locus_tag="ACHOQV_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFP9628594.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(59379..60107)
                     /gene="mtgA"
                     /locus_tag="ACHOQV_000162"
     CDS             complement(59379..60107)
                     /gene="mtgA"
                     /locus_tag="ACHOQV_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFP9628595.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(60104..60757)
                     /gene="elbB"
                     /locus_tag="ACHOQV_000163"
     CDS             complement(60104..60757)
                     /gene="elbB"
                     /locus_tag="ACHOQV_000163"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFP9628596.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60987..63323)
                     /gene="arcB"
                     /locus_tag="ACHOQV_000164"
     CDS             complement(60987..63323)
                     /gene="arcB"
                     /locus_tag="ACHOQV_000164"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFP9628597.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLALVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(63419..64348)
                     /gene="yhcC"
                     /locus_tag="ACHOQV_000165"
     CDS             complement(63419..64348)
                     /gene="yhcC"
                     /locus_tag="ACHOQV_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP9628598.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKPTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQTECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            65023..69483
                     /gene="gltB"
                     /locus_tag="ACHOQV_000166"
     CDS             65023..69483
                     /gene="gltB"
                     /locus_tag="ACHOQV_000166"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFP9628599.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            69496..70914
                     /gene="gltD"
                     /locus_tag="ACHOQV_000167"
     CDS             69496..70914
                     /gene="gltD"
                     /locus_tag="ACHOQV_000167"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFP9628600.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDLSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEDNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            71097..72224
                     /gene="yhcG"
                     /locus_tag="ACHOQV_000168"
     CDS             71097..72224
                     /gene="yhcG"
                     /locus_tag="ACHOQV_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFP9628601.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNPDARSFYE
                     KETLRCGWSVRQLERQITTQFYERTLLSHDKSAMLQQHAPAEMHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(72284..72748)
                     /gene="nanQ"
                     /locus_tag="ACHOQV_000169"
     CDS             complement(72284..72748)
                     /gene="nanQ"
                     /locus_tag="ACHOQV_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFP9628602.1"
                     /translation="MIMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQCDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(72745..73620)
                     /gene="nanK"
                     /locus_tag="ACHOQV_000170"
     CDS             complement(72745..73620)
                     /gene="nanK"
                     /locus_tag="ACHOQV_000170"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP9628603.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALHDA
                     LSALVSPLQVHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDITEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHCSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(73617..74306)
                     /gene="nanE"
                     /locus_tag="ACHOQV_000171"
     CDS             complement(73617..74306)
                     /gene="nanE"
                     /locus_tag="ACHOQV_000171"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9628604.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(74354..75844)
                     /gene="nanT"
                     /locus_tag="ACHOQV_000172"
     CDS             complement(74354..75844)
                     /gene="nanT"
                     /locus_tag="ACHOQV_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFP9628605.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            complement(75954..76847)
                     /gene="nanA"
                     /locus_tag="ACHOQV_000173"
     CDS             complement(75954..76847)
                     /gene="nanA"
                     /locus_tag="ACHOQV_000173"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP9628606.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(76969..77760)
                     /gene="nanR"
                     /locus_tag="ACHOQV_000174"
     CDS             complement(76969..77760)
                     /gene="nanR"
                     /locus_tag="ACHOQV_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP9628607.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        18976 a        21125 c        19465 g        18331 t
ORIGIN      
        1 aatgagcagt ccaaatattc tcttaacccg tattgataac cgtctggttc acggtcaggt
       61 tggcgtgacc tggacatcca ccatcggtgc gaatctgctg gtagtcgtgg atgatgttgt
      121 cgctaacgat gatattcaac agaaattaat gagtattacc gcagaaacct acggctttgg
      181 cattcgtttc tttactatcg aaaaaaccat taacgtcatc ggcaaagctg caccgcatca
      241 aaagatcttc ctgatttgcc gtacgccaca aacggtacgt aaattggtag aaggtggtat
      301 tgacctgaaa gatgtcaacg tcggcaatat gcatttctcg gaagggaaaa agcaaatcag
      361 cagtaaagtt tatgtcgatg accaggatct cacggactta cgttttatta aacaacgtgg
      421 cgtgaatgtt ttcattcagg acgtccctgg cgatcaaaaa gaacaaatcc ctgactaaat
      481 ctaaaatcgc cttaatattg gtttgaggta ataaaaatgc atgaaataac cctacttcag
      541 ggattatccc tggcggcgtt agttttcgtt ctggggattg atttttggct ggaagcctta
      601 tttttattcc gcccgataat cgtttgtacc ctaactggcg ctattctcgg tgatattcag
      661 actggcttaa ttaccggtgg tctgacagag ttggctttcg ccggattaac ccctgcaggt
      721 ggtgttcagc cgcccaaccc gattatggcg ggtctgatga ccaccgtcat tgcatggtct
      781 acgggcgttg atgccaaaac agcaattggt cttggcctgc cgtttagttt gttaatgcag
      841 tatgtcattc tgttcttcta ttctgctttc tcattattta tgaccaaagc cgataaatgc
      901 gcgaaagaag cggatacggc agcgttttcc cgacttaact ggacaacgat gctcatcgtt
      961 gcttcagcgt atgcggtgat tgctttcctc tgtacttacc tggcacaagg ggcgatgcag
     1021 gcgctggtga aagcgatgcc cgcctggctg acccacggct ttgaagtggc tggcggtatt
     1081 ctgcctgccg ttggttttgg cttgctgctg cgcgtgatgt tcaaagcgca atatatccct
     1141 tacctgatcg ccggcttcct gtttgtttgc tacatccagg tcagcaacct gttgccggtt
     1201 gccgtactgg gcgcaggctt tgcggtgtat gagtttttca atgcgaaatc ccggcagcaa
     1261 gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg actacagcaa tgggatctga
     1321 aatcagtaaa aaagatatca cccgtctggg ctttcgttcg tcactgctac aagcaagctt
     1381 taactacgaa aggatgcagg cgggcggctt tacctgggcg atgttgccgc tcctgaaaaa
     1441 gatttataag gacgacaaac caggcctaag cgcggcgatg aaagataacc tagaatttat
     1501 taatacccac ccgaatctgg tcggattcct gatggggtta ttaatttcga tggaagagaa
     1561 aggggaaaac cgcgacacca ttaaaggcct caaagtggcg ctgtttggcc caatcgccgg
     1621 gattggcgat gcgattttct ggtttacctt gttaccaatt atggcgggga tttgctcatc
     1681 gtttgccagt cagggaaacc tgctggggcc gattctgttc tttgccgttt acctgcttat
     1741 ctttttcttg cgcgtcggct ggactcacat cggttactcg gtcggcgtga aggctattga
     1801 taaagtgcga gagaactcgc agatgattgc ccgttcggca accatcctcg ggatcacggt
     1861 aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg gtgacatcgt ttgccatcga
     1921 cagtacccac agcgtcgcgc tgcagcagga tttcttcgat aaagtcttcc cgaacatttt
     1981 accgatggcc tacaccctgc tgatgtatta cttcctgcgg gtgaaaaaag cgcatccggt
     2041 gctgttaatc ggcgtgacgt ttgttctttc tattgtttgt tctgcattcg gcattttgta
     2101 aatggaacga ggcaccgcgt ctggcggtgc ctctttactt aaggaatttc atcctgtgca
     2161 aacccttcaa caagttgaaa actatacggc gttaagtgaa cgtgccagcg aatatttatt
     2221 ggccgtgatc cgtagcaaac cggatgccgt gatttgcctg gcgaccggag ccacgccatt
     2281 actgacgtat cattatctgg tagaaaaaat ccaccagcag caggttgatg tcagccagct
     2341 caccttcgtg aagctcgacg aatgggtgga tctgccatta acgatgcctg gtacctgcga
     2401 aactttcctg caacagcata tcgtgcagcc gctggggcta cgtgaagacc agctcatcag
     2461 ctttcgctcc gaagagatta atgagacaga gtgcgaacgg gtaacgaacc tgattgcgcg
     2521 caaaggcggt ctggatttat gcgttctcgg gttggggaaa aacggtcatc ttgggctgaa
     2581 cgaaccggga gaaagcctgc aaccggcctg ccatatcagt caacttgatg ccagaacaca
     2641 gcaacatgag atgttaaaaa ccgcgggccg tcctgtgact cgtgggatca ccttaggcct
     2701 gaaggatatt ctcaatgccc gcgaagtttt gttactggtg actggcgaag gaaagcagga
     2761 tgcgacagaa cgttttctca cggctaaagt ctctaccgct atcccggctt catttttatg
     2821 gctgcataac tatttcacat gcatgattga tgagatgtgt cgtcgttaac tcgcctgcta
     2881 caggcgttgc aacgctattc ctgctgcgac gcctgtaatc actctccctg ctgctccagc
     2941 gcatacttat acagcgcatt tttcttcacg ccgtgaatct ctgccgccag cgccgccgct
     3001 tttttcagcg gcagttctgc ctgtagcagc gccagcgtgc gcagggcatc ggcgggtaag
     3061 tcttcttcct gcgctttatg gccttcgaca atcagcacca tttcgccttt gcgacggttt
     3121 tcatcttctt tgacccacgc cagcagctcg ccaacgggcg cgccgtgaat ggtttcccag
     3181 gttttggtca gctcacgcgc cagaaccacg tagcgggatt cgcctaatac cgcaacgata
     3241 tcttccaggc tatctaacag acggtgggta gattcataaa aaatcagcgt gcgcggctcc
     3301 gcttcaatag cttttagcgc atcacggcgg ccttttgatt tggcaggtaa aaagccttca
     3361 taacaaaaac gatcggatgg taaacccgcg gcgcttaacg cagtgatagc agcacacggc
     3421 cctggtagcg gcaccacgcg aatccccgct tcacgacagg tacgcaccag atggtagcca
     3481 ggatcgttaa ttagcggcgt tccggcatcg gaaaccagcg caatgttttg cccctcttgc
     3541 agtttcgcca gcagcgtttc ggctttttgt tgttcgttat ggtcgtgcag cgcaaacaac
     3601 cgggcattaa tcccaaaatg ttgcagcaat aaaccggtgt gacgcgtatc ctcggcggca
     3661 atcagatcaa cggcctgtaa tacctctaat gcacgctggg tgatatcagc cagattgccg
     3721 attggcgtcg gtacaatgta aagctggccc tgagaattat ccgccgattg gtgttgtttc
     3781 attgtgtcgt ccgtattgcc gatttaatat tgagcattgc gtaaaaaaaa tatcactgga
     3841 tacattatgg taccctcaac attttctcgt ttgaaagccg cgcgttgtct gcctgttgtt
     3901 ctggcagccc tgattttcgc cggttgtggc acccatactc ccgatcagtc cactgcttat
     3961 atgcagggca cggcgcaggc tgattctgcc ttttacctgc aacagatgca gcaaagttct
     4021 gatgatacca ggatcaactg gcaattactc gccattcgtg cactggtgaa agaaggtaaa
     4081 accggacagg cggtggagtt gtttaaccaa ctaccgcaag aactgaacga ttctcagcgt
     4141 cgcgagaaaa cactgctggc ggcagagatt aaactggcgc agaaagattt tgctggcgcg
     4201 caaaacttgc tggcgaaaat cacacctgcc gatttagaac aaaaccagca agcccgttac
     4261 tggcaggcaa aaatcgatgc cagccagggg cgtccttcca ttgatttact gcgcgcgtta
     4321 attgctcagg aaccgctgct cggtgcgaaa gaaaagcagc agaatattga cgccacatgg
     4381 caggcgctct cctccatgac tcaggagcag gcgaatacgc tggtgatcaa cgccgacgaa
     4441 aatattctgc aaggctggct ggatttgcag cgcgtctggt ttgataaccg taacgatcct
     4501 gacatgatga aagctgggat cgccgactgg cagaaacgtt atccgaacaa cccgggcgcg
     4561 aaaatgctac caacgcagtt ggttaacgta aaagcgttta aacccgcatc gaccaacaaa
     4621 atcgccctgc tgttgccgct gaatggccag gcagcggtat ttggtcgcac tattcagcaa
     4681 ggctttgaag cggcgaaaaa tatcggcact cagccagtgg cggcccaggt agcagccgca
     4741 cctgccgcag acgtagcaga acaacctcag ccgcaaaccg tggatggcgt tgccagcccg
     4801 gcacaagctt cggttagcga gctgaccggt gaacagcctg cggcccagcc ggtacctgta
     4861 agcgccccgg cgacaagcac cgcagcggta agcgcacccg caaatccatc cgcagagctg
     4921 aaaatctacg atacctcatc acaaccactt agccagatct taagccaggt tcagcaggat
     4981 ggtgcgagta ttgtggtcgg tccgttgctg aaaaataacg ttgaagagtt gctgaagagc
     5041 aacaccccgc tgaacgtgct ggcactgaac cagccggaga acatcgaaaa ccgcgtcaat
     5101 atttgttact tcgcgctttc accggaagac gaagcgcgcg atgcagcgcg tcatattcgt
     5161 gaccagggta aacaagcgcc gctggtgctg atcccacgca gttcattggg cgaccgcgta
     5221 gccaatgcgt ttgcgcaaga gtggcagaaa ctgggcggcg gcaccgttct gcaacaaaaa
     5281 tttggttcca ccagcgaatt acgcgcgggt gttaacggcg gttctggtat tgctttaacg
     5341 ggtagcccga ttactcccag agcgacaacc gactccggca tgacgaccaa caatccaacg
     5401 ctgcaaacca cgccaaccga tgaccagttc accaataatg gcggtcgtgt cgatgcggtg
     5461 tacattgtgg caacgccggg tgaaatcgct tttatcaaac cgatgatcgc catgcgtaac
     5521 ggtagccaga gcggtgcaac gctgtacgcc agttcccgca gcgcacaagg caccgcaggc
     5581 ccggatttcc gtctggagat ggaaggcttg cagtatagcg aaatcccgat gctggcaggc
     5641 ggtaatctgc cgttaatgca gcaggcgctc agcgcggtga acaacgatta ttcgctggct
     5701 cgcatgtatg cgatgggcgt cgatgcctgg tcgttggcaa atcatttctc acaaatgcgc
     5761 caggttcagg gttttgaaat caacggtaat accggaagtc tgacggctaa cccggattgc
     5821 gtgattaaca ggaagttatc atggctacag taccaacaag gtcaggtagt ccccgccagt
     5881 taaccaccaa acagaccggc gatgtgtggg aagcacaagc gcgtcgctgg ctggaaggca
     5941 aaggactgcg gtttatcgcc gctaacgtga acgagcgtgg cggcgagatc gatctgataa
     6001 tgcgtgaagg ccggatcacc gtttttgtcg aggtgcgtta ccgccgctct gcgctttatg
     6061 gcggcgcggc agccagtgtg acccgcagca aacaacacaa attattacag actgcccgct
     6121 tgtggctcgc gcgtcataat gggagttttg atactgtgga ttgccggttc gatgtggtag
     6181 ccttcaccgg gaatgaggtt gagtggatta aggatgcctt taatgaccac tcataattaa
     6241 ggtttaagga ttagcgtgca agaaagaatt aaagcttgct tcactgaaag cattcaaact
     6301 caaattgcgg cggcagaggc gcttccggat gccatctcgc gtgcagccat gacgctggtt
     6361 cagtctctgc tcaatggcaa caaaatcctc tgttgtggta atggaacttc cgctgccaat
     6421 gcacagcatt ttgctgccag catgatcaac cgtttcgaaa cggagcggcc cagcttacct
     6481 gccattgcac taaatactga taatgttgtc ttaacggcga ttgccaacga tcgcttacat
     6541 gatgaagtgt atgcaaaaca ggtgcgggcg ctgggtcatg cgggagatgt attgttagcc
     6601 atttccaccc gtggcaacag ccgcgatatt gtaaaagcgg tagaagctgc cgttacgcgt
     6661 gatatgacca ttgtggcatt aaccggctat gacggcggtg aacttgcagg tttgttaggg
     6721 ccacaggatg tggagatccg cattccttcg catcgtagtg ctcgcattca ggaaatgcat
     6781 atgctgacgg taaattgcct gtgcgatctg atcgataaca cgcttttccc tcaccaggat
     6841 gtttaaggag aatacatgaa ggcattatcg ccaatcgcag tccttctttc cgcgctgctg
     6901 ttgcaaggtt gtgttgccgc tgccgtagtg ggtaccgctg ctgtaggtac caaagccgca
     6961 actgacccac gcagtgtcgg cacccaggtg gacgatggta ccctggaagt gcgcgtgaac
     7021 agcgcattgt cgaaagacga acaaattaag aaagaagcgc gcattaatgt cacggcctat
     7081 cagggcaaag tgctgctggt tgggcagtca ccaaacgctg aactttcggc tcgcgccaaa
     7141 cagattgcta tgggcgtaga cggtgccaac gaagtgtata acgagattcg tcagggccag
     7201 ccgattggtc tgggcgaagc gtctaacgat acgtggatca ccaccaaagt acgttcgcag
     7261 ctgttaacca gcgacctggt gaaatcgtcc aacgtgaaag tgaccaccga aaacggtgaa
     7321 gtgttcctga tggggctggt gactgaacgt gaagcgaaag cggcggcaga tattgccagc
     7381 cgggtaagcg gcgtgaagcg ggtaactacg gcgtttacgt ttattaaata gcagcccttg
     7441 taatgcctga tgcgacgctt gcagcgtctt atcaggccta caacgatgca aaccgtaggt
     7501 cggataaggc gtttacgccg catccgacat gttttccctc aaatcaaacc aacagcgcca
     7561 gcccgccgac aatcacacca gacactgcca ccatcgcccc cgtcagccat aaggctttcg
     7621 ccgggaacgc tttgcgcagc ataatcagtg acggcaaact caccgccggg agcgtcatca
     7681 acaatgccag cgccggagca gttcccatac ctgccagcat catcgtttgt acaatcggaa
     7741 tttctgctgc cgtgggaatg acaaacaagc atcccgctac cgccatcgcc accacccaca
     7801 tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcga gcggcaccca
     7861 acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagcgtc catagcgccc
     7921 tgccccagcg gctaaaaaat cctccctgtg cttccggtat gtcaatttcg accggtgcct
     7981 gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc aacaccatca
     8041 ccagccccgc caccagacga atcgccgcaa aaccccagcc gaggacgaac cccataaaca
     8101 ccagcgtcgc cgggtttaac aacggattcc ccatccagaa tgccagcgca ccgcccatcg
     8161 acacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta cacatcatgc
     8221 ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac
     8281 gcaataacca gtcacgcggg atcagcacct gaatcaacga cccgagcagt acgcccagca
     8341 ccgccgcttt ccataccgcg aggaaataga tcatcgcgta atccaacgcc gcctgccacg
     8401 gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg gcagcagtaa
     8461 acgctttgcc gtagtaaggt tgccatttca cataccagag accagcaatg acaacgagaa
     8521 agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa gactgaccag
     8581 tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat
     8641 caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca
     8701 caccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg tctctcgcat
     8761 caatggattt ccagttaccc ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca
     8821 tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc
     8881 agttctgggc gattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg
     8941 agtgggcatt agcacccata gcactgacca ccagcatatg ctgcgcaccg agccgccgcc
     9001 cggttaatgc ggtatccacc accagcgtgt aatcggcatg aataaacgcc tctttactcc
     9061 ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatggga tcggtgacct
     9121 gcgccagcgc gtcggtcagt tgcggatcat ggggattaaa cacaccgggc atatcgccca
     9181 acggacgtcg cgtcggcgcg gctatggcgt taactttcgg ttcattaatc aacatccgca
     9241 gcaggtgacc acccaccagc cccgttgcgc ctgtaatcag tacctggctc atcttcgctc
     9301 ctttacagaa ttgtctgcct tgcgctcccc ggctcatgca ccaggcttaa tagtctacga
     9361 ggtaattatc cccgattgtg ggaaattcgc ctcatccaat gcaacaacgc ggaggaagca
     9421 tgagtaagaa aattgccgtt ttaatcactg atgaatttga ggattcagaa tttacttcac
     9481 ccgcagacga gttccgtaaa gccggacacg aagtgattac cattgaaaaa caagcgggta
     9541 aaacggtgaa aggcaaaaaa ggagaagcca gcgtggccat cgataaatcc atcgatgaag
     9601 tgacgccagc ggagtttgat gccctgctgc taccgggcgg ccattcaccg gattatctgc
     9661 gtggcgacaa ccgttttgtc actttcaccc gtgattttgt gaatagcggc aaacctgttt
     9721 tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg gggcgcaaac
     9781 tgactgccgt taaaccgatc attattgatg ttaaaaacgc gggcgcggaa ttttacgatc
     9841 aggaagtggt ggtcgataaa gatcagctgg ttaccagccg tacaccggac gatctgccag
     9901 cgtttaaccg cgaggcatta cgcctgctcg gtgcctgagt tacgtagcca gatcattttt
     9961 ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccacacc
    10021 ggggccgaca gcattctggc aaccggaaag cggcgattgt acgcctggcg cgcaaggtcg
    10081 ctttcctcac cttcaagcct gcggatctcg cctttaaatt gcacgccgcg aattaacgct
    10141 accgttttcg gctgaccgtt taccgttccg gcaactgccg cctgcggccc actcatctgc
    10201 gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaaaaga
    10261 taaaaggcat tagcgcacca gagttcccct tcctgctgca cacaccaggt gacaacatgt
    10321 tgttttgcca gccaacggct gatggcggtg agtgtttcca ttgctgttct cttttatact
    10381 gtgggcctga ccttaacatg cgacgcagac gatgacaccc tggtttcttt acttgatccg
    10441 taccgccgac gataagcttt ataccgggat caccacagat gtcgaacgcc gctatcagca
    10501 gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactaa cgctggcgtt
    10561 ttccgcgcca gtgggcgatc gttcgctggc actacgggcg gaatatcgcg ttaagcaact
    10621 gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag aactgttgag
    10681 cagcctgcaa accccaaaga ttaaaagcga ttgaagtgct cgtgatactc aaccagacca
    10741 gttacaccgt tcagcgcgtc atccgctaaa cgatgtacct ggaaggcact ttcagtgccc
    10801 ggccagcggc aacgcagatc gtgatgtgcc gccagttcaa agccgaaacg gctgtacagc
    10861 gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct
    10921 tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatctac cgccagcggt
    10981 gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag
    11041 ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca
    11101 cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgaacgacg cagcagggca
    11161 tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg
    11221 tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca
    11281 gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca
    11341 catacagccc cagctccgta tcgccttcaa ttaccagccg acgctggaag aagagcgtat
    11401 ccggatcttg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca ctaaaactca
    11461 catcagcttg cgcgttctgg ctaacgacca gtttgccatt caccaccgag gtaaaccatt
    11521 gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac tccagctcgc
    11581 catcatccaa cgcctggcgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta
    11641 gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac
    11701 gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc
    11761 catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc
    11821 accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc
    11881 cagttcgtta acatttttgc gctttgatag cgcaaccttc aggaaaaatt atggagctgc
    11941 tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg
    12001 tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg
    12061 agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg
    12121 cgattaacac ttttgcgcat ccggacggtt acgcccgctg gcagcgcgcc gtggatatgg
    12181 cggcgcagtt gggtgccgac gcgctgatcc tcgccgacct cgccatgctg gagtacgccg
    12241 ccgagcgtta cccgcatatt gagcgtcatg tgtcagtgca ggcttcggcg accaatgaag
    12301 aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt
    12361 tgtcgattca tcaggtgaaa caactggcac gggtcacacc tgtcccgctg gaagtgtttg
    12421 ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg tatctgacgg
    12481 gtgagtcgcc caacaccgtg ggcgcgtgtt ctccggcccg tttcgtgcgc tggcagcaaa
    12541 cgccgcaggg actggaatct cgcctgaacg aagtgctgat cgaccgttat caggatggcg
    12601 aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc
    12661 acgcgctgga agaacccacc agtctcaata ccctggaact gctgccggag ttaatggcgg
    12721 cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg
    12781 tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa aacttcgtac
    12841 cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcaa actacccttg
    12901 gcgcgtatca ccgtaaatgg cagtgagaaa agcaatgaaa tattccttag ggccggtgct
    12961 atggtactgg ccaaaagaga cgctggaaga cttttatcag caggccgccg ccagcagcgc
    13021 agacgtgatt tatcttggtg aagcggtctg tagcaaacgc cgggcaacca aagttggcga
    13081 ctggctggag atggcaaaat cgctcgccgg aagtggcaag cagattgtgc tctccacgct
    13141 ggcgctggtg caggcgtcct ccgaacttgg cgaactgaaa cgttatgttg agaacggtga
    13201 gtttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac gcaaactgcc
    13261 gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg acactgaaaa tattgctcaa
    13321 acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc tggtgaatct
    13381 gcttaatcag tgcgatgagc tgggcattcg caaccagttt gaagtggaag tcctgagcta
    13441 cggtcatctg ccgctggcct actccgcccg ctgctttacc gcgcgttcgg aagaccgccc
    13501 gaaagatgag tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg tgctatcgca
    13561 ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct acgtttacaa
    13621 cctcggtaac gagctggcat ccatgcaggg gctggtcgat gtggtccgcc tgtcaccgca
    13681 gggtactgac actttcgcga tactcgacgc cttccgcgcc aatgaaaatg gcgctgcacc
    13741 actgccgttg acggcaaaca gcgattgtaa cggctactgg cggcggctgg ccgggctgga
    13801 gctgcaagcc taagtaaata gctcactttg ttaacaactt taactactct ttaatgcagt
    13861 attaaagatt aatcagtaac aaagtgagct gttatgactg ataaaaccat tgcgttttcg
    13921 ctactcgatc tggcccccat tcccgaaggc tcttcagcgc gggaagcgtt ctcccactct
    13981 ctcgacctcg cccgtctggc tgaaaagcgc ggctatcatc gctactggct ggcggaacac
    14041 cacaatatga ccggcattgc cagtgctgca acatcagttt tgattggcta tctggcggcg
    14101 aataccacta cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca ctcaccattg
    14161 gtgatcgccg aacagttcgg cacgcttaat acgctctatc cggggcgaat cgatttgggg
    14221 ctgggtcgtg cgccgggtag tgaccagcga accatgatgg cactacgtcg tcatatgagc
    14281 ggtgatattg ataatttccc ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc
    14341 gatcccaatc cgcatgtgcg cccggtacca ggctatggcg agaaaatccc cgtgtggttg
    14401 ttaggctcca gcctttacag cgcgcaactg gcggcgcagc ttggtctgcc gtttgcgttt
    14461 gcctcacact tcgcgccgga tatgttgttc caggcgctgc atctttatcg cagcaacttc
    14521 aaaccgtcgg cacgactgga aaaaccatac gcgatggtgt gcatcaatat tatcgctgcc
    14581 gacagcaacc gcgacgctga atttctgttt acctcaatgc agcaagcctt tgtgaaacta
    14641 cgccgtggcg aaaccggaca actaccgcca ccgattcaaa atatggatca gttctggtca
    14701 ccgtctgagc agtatggcgt gcagcaggcg ctgagtatgt cgttggtagg tgataaagcg
    14761 aaagtgcgtc atggcttgca gtcgatcctg cgcgaaaccg acgccgatga gattatggtt
    14821 aacgggcaga ttttcgacca ccaggcacgc ctgcattcgt ttgagctggc gatggatgtg
    14881 aaggaagagt tgttggggta gtgtgtttta acgtaggaag ccttatccga gatggcaacg
    14941 ctgtcctacg taggcctgat aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt
    15001 cggataaggc accgctgatt actgatacac cggcagcaaa ttaaagctcg ataagatatg
    15061 gaccagtgcg ttgccaacgc caaataccag aatcagcata atcatcggct tgccgcccca
    15121 gacgcggaat ttcgggctac caaagcgttt acgcgatgcg cgggccaaca gcgccggaac
    15181 aattgccgcc cagatggtag ccgctaagcc agcataacca atggcgtaca ggaatccgtt
    15241 aggaaacagc aggccaccca caacaggcgg ggcaaaggtc agcaacgctg ttttcaaacg
    15301 gcccatagcc gagtcgtcga aaccaaacag atctgccaga tagtcaaaca aacccagcgt
    15361 tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac acgaccagca gaagatccag
    15421 gctacggctg ttcagcacgc cgcttaatgc ctgtaccagc acatcaatat taccgccctt
    15481 ctcggcgatg ccgataaact ccggacgagg gatgttgccc atcgtcgcca gcaaccagat
    15541 ggtatacagc gccagcgcca tcagcgtacc gtacaccagg catttcacga tggttttcgg
    15601 atctttgccg taatacttca tcaggctcgg cacgttaccg tgataaccaa acgatgccag
    15661 acagaatggc agtgtcatca gcagatacgg cgcataagac gcattgcttt cggcgacgtt
    15721 gaacaaggtc gttggctgta catgccccag caggctgccg aaggtgagga agaaagtaat
    15781 gactttcgcc cccagcacaa ttgctgtcat ccggctgaca gctttcgtac tcaaccacac
    15841 cacgaacgct accagcagtg caaaaccaaa tccagctgcc cgtgcaggga cgttcagcga
    15901 catctccgcg aaggtgtgat gcagaatcga accgctggca gaaatatagg cgtaagtcag
    15961 gatatagagc acaaaggcaa tggaaatgcc gttgactacg ttccagcctt tgcccagcaa
    16021 atctttggtg atggtgtcaa agctagaacc gattcgataa ttcaggttag cttccagaat
    16081 catcaagccg gaatgcagca tacagaacca ggtaaagatc agcgccgcca ttgaccagaa
    16141 gaaccacgcc ccggacatga ccacaggcag agaaaacatc cctgcgccaa taatggtgcc
    16201 gccgataatc accacgccgc caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc
    16261 catgagggct tctctccagt gaaaaatagt gcgactgcgt tgttatgcat tgcactgtac
    16321 cagtacacga gtacaaaaga cagaaaaaaa gccccgatgg taaaaatcgg ggctgtatat
    16381 attattttac agattgtgtt cgctgttcag cgatgattac gcatcaccac cgaaacgacg
    16441 acgaccggta gagtcatcac gacgcggagc gcggccttca cgacgttcgc cgctaaaacg
    16501 acgaccatca ccacgaccac cttcacggcg ttcaccgctg aagttacgac cgccttcacg
    16561 acgttcgcca ctgaaaccac gaccaccgcc acgacgctca ccgccagtat gcggctgtgc
    16621 atcgcccagt aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg taaagtgttg
    16681 cagcacttca cccggcatac ctttcggcag ttcgatggtg gagtgagaag caaacagctt
    16741 gatgttacca atgtaacggc tgctgatgtc gccttcgtta gcaatcgcac caacgatatg
    16801 acgaacttca acaccatcat cgcggcccac ttcaatgcgg tacagctgca tatcgccaac
    16861 atcacgacgt tcacgacgcg gacgatcttc acggtcacca cgcgggccac ggtcgttacg
    16921 atcgcgcgga ccacggtcat cacggtcacg gaattcacgt ttcggacgca tcggcgcatc
    16981 tggcggaaca atcagagtac gttcaccctg tgccattttc agcagtgcgg cggccagagt
    17041 ttcgagatcc agctcttcac cttctgccgt cggctgaatt ttgctcagca gtgcacggta
    17101 ttgatccaga tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt tttccagacg
    17161 acgtttgccc agcagttctg cgttcggcag ttcaacttcc ggaatggtca gcttcatggt
    17221 gcgttcaatg ttacgcagca gacgacgctc acggttctca acgaacagca gcgcacggcc
    17281 agcacggccc gcacgaccag tacgaccgat acggtgaacg taagactcag aatccatcgg
    17341 gatatcgtag ttaactacca ggctgatacg ctcaacgtcc aggccacggg ccgcaacgtc
    17401 ggtcgcaatc aggatgtcca gacgaccatc tttcagtcgt tccagtgtct gctcacgcag
    17461 cgcctggttc atgtcaccgt tcagcgcggc gctgttgtag ccgttacgct caagggcttc
    17521 tgccacttcc agagtcgcgt ttttggtacg aacgaagata atcgccgcat caaaatcttc
    17581 cgcttccagg aaacgtacca gtgcttcgtt tttacgcata ccccagacag tccagtagct
    17641 ctggctgatg tcaggacggg tagtcacgct ggactgaatg cgcacttcct gcggctcttt
    17701 cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga acagagcggt
    17761 ctgatgacct tccgggatct gcgccataat ggtttcaacg tcttcgatga agcccatgcg
    17821 cagcatttcg tcagcttcat ccagaaccag accgctcagt ttagagaggt ccagagtgcc
    17881 acgtttcagg tggtccagca gacggcccgg agtaccgaca acgatctgcg gcccctgacg
    17941 cagggcgcgt aattgcacgt cataacgttg gccgccgtac agggcaacca cgtttacgcc
    18001 gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt
    18061 cggtgccagc accagaatct gtggtgcttt cagctcagga tcaagattct gcagcagagg
    18121 taaagagaat gctgcagttt ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc
    18181 attcagcaga tgtggaatac actctgcctg aattggagat ggtttttcgt aacccagatc
    18241 gttaagggct tcaaggatag gagccttcag gcccagatct gcaaaagtgg tttcgaattc
    18301 agccatgtag tacgtgtgcc tcaaaattaa tggcggccag tctacataac tcatcatgaa
    18361 attgatcagc aattttcatt gaaaagtgtg aaccggctca aagtaggtgt attaacgaac
    18421 aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac
    18481 gtcagctatt gctggtccga ttctgccagg tcatcttggt cctggcccag gagcgataat
    18541 tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg
    18601 aacagtgccg tggcgctgtc caaatccccc agacttaggt agtacttacc taaatagaag
    18661 ttggtttcac tgagatgctc agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt
    18721 tccattaacg tttgttcgct aatgttgccc aggtagaact cgacaatgtt ccatccccac
    18781 tgttccttat ccgatttttc gaagtgctgt ttcaacactt ctttagcctg cttctcatcg
    18841 agcttctgct cggcgagata aagccacaga ctacggaaag gatcattggg atcgtcttga
    18901 taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata taatgcgatc
    18961 ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct caagtacaga atcaaacgct
    19021 tcataggcag catcaaaatt gcctgcctgc gttaaatata tgcctaagta attgaatact
    19081 tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc
    19141 agaccgagac tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg
    19201 gttaaagccc gactggcaag gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa
    19261 gtcggttgca atggtaccgc gaggacttca cttttacgcc aggaagtatt actgcatcct
    19321 gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc
    19381 cgaagaccac ggttgaatga acgtcctgtt cccggttgct aacaaggcgt cctgcccggt
    19441 taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt actcgccctg ttcagcagcc
    19501 ggagcttccg gtgctgcagc aggttgagac tgctcagtcg cttctttaat gctcagacgg
    19561 atacggccct ggcgatcaac ttccagaact ttcaccggta cttcctgacc catctgcagg
    19621 taatcggtca ctttctcaac gcgtttgtca gcgatctgag agatgtgtac cagaccttct
    19681 ttaccgccgc cgatggcaac aaatgcgcca aagtcaacga tacgggtcac tttaccattg
    19741 tagacgcggc ccacttcgat ttctgcagtg atctcttcga tacgacgaat agcatgtttc
    19801 gctttctcgc cgtcggtcgc tgcgattttc acagtaccgt catcttcgat ttcgatggtg
    19861 gtgccagttt cttcggtcag agcacggata acagaaccgc ctttaccgat aacgtctttg
    19921 atcttgtccg ggttgatctt gatggtatgg atacgcggag cgaactcaga gatatcgccg
    19981 cgcggcgcgt tgatcgcctg ttccattacg cccaggatat gcagacgcgc acctttagcc
    20041 tggttcagcg caacctgcat gatctctttg gtgatacctt caattttgat atccatctgc
    20101 aatgcagaga taccgtcacg ggaacccgca actttgaagt ccatatcgcc caggtgatct
    20161 tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc cttctttcac cagacccatt
    20221 gcgatacccg caacggcagc tttgatcggc acacctgcgt ccatcagcgc cagagacgcg
    20281 ccgcacacgg aagccataga agaggaaccg ttggattcgg tgatttcaga cacaacacgt
    20341 acggtatacg ggaatttgtc catatccggc atgactgcca gcacgccgcg cttcgccaga
    20401 cgaccgtgac caatttcacg acgcttcgga gaaccgacca tgccggtttc gcctacagag
    20461 tacggaggga agttgtagtg gaacaggaag gtgtcagtac gttcgcccat cagttcatca
    20521 agaacctgcg cgtcacgtgc agtacctagc gttgcggtaa ccagcgcctg agtttcacca
    20581 cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacac cagtacgcac atccagacca
    20641 cggatcatat ctttttcacg accgtcgata cgcggttcgc ctgccagtac gcggctacga
    20701 acaacatttt tctcgatagc gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg
    20761 tcttcagcaa gcagtgtcgc gatggtttca gatttgatga catcaacctg cgcataacgc
    20821 tcttgtttgt cggtgatgcg gtaagcatcg ctcagacgag cttcagccag tgcagcaacg
    20881 cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt cccaacgcgg tttaccggct
    20941 tctttcacca gttcattgat gttctgaata acaacctgct gttgttcatg accgaagact
    21001 actgcgccca gcatctggtc ttcgctcagc agttcagctt cagattcaac catcagtaca
    21061 gcggcttcag tacccgcaac aaccagatcc agtttgctct ctttcaactc gtcctgagtc
    21121 gggttcagta cgtactggtc attgatgtaa cctacacggg cagcaccaat cgggccattg
    21181 aacggaatac cagacagaga cagggctgcg gaagcaccaa tcatcgcgac gatatccggg
    21241 ttaacctgcg ggttaacaga aaccacggtg gcgataacct gaacttcgtt gacgaagcct
    21301 tccgggaaca gcgggcgaat cgggcggtca atcagacgcg cgatcagggt ttcgccttcg
    21361 cttgggcggc cttcacgacg gaagaagcta cccgggatac gaccagcagc gtaggtacgc
    21421 tcctgatagt taacggtcag cgggaagaag tcctgacctg gtttggcttt tttctgacca
    21481 acaacggtaa cgaataccgc agtgtcatcc atgctaacca taacagcggc agtagcctga
    21541 cgagccatca tgccggtttc cagagtcacg gtgtgttggc cgtactggaa tttacgaacg
    21601 atcggattaa gcaatgtaat atcctttctc tttcttagac agtaccttac ggcactggtg
    21661 ttaatacccg atcttctgcg catcctcgcg actaatgaca accctaaccc agctctatgt
    21721 gggtaaagcc tctcattagc cgcgcgaacc tctgcagcgg aagatcattc atagcaacaa
    21781 tacattagtt tccagtgaat tgctgccgtc agcttgaaaa aaggggccac tcaggccccc
    21841 ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag ctgggtgtaa
    21901 cgtgctacgt ctttacgttt caggtagtcg agcagtttac gacgctgaga aaccatgcgc
    21961 agcagaccac gacggctgtg gtgatctttt ttgtgctctg caaagtggcc ctgcaggtgg
    22021 ttgatctgtg cagtcagcag tgctacctga acttcggtag aaccggtgtc gtttgcgtca
    22081 cgaccaaact cagaaacgat tttagctgtt gcttcagtac ttagagacat tttaaaactc
    22141 caaagtatat agaatgaaag gacgccgatc tctaattcag cgatcccagt gtacgttacg
    22201 caaagtgtta aacaatttac gcgacgttaa gcggcagtat tctactcgta gcgacttgtt
    22261 atcgcaagac ggttaacatt acgccgggta ttcaaccacc aggcgacgag gcgcaacgcg
    22321 gccttcatcg tcaatttcgc ccataccgat aaacttgccg ttctcaccct ctgtaacgcg
    22381 aaccagtcct tccagcggcg caccggatgt acgaactggg ttaccatttt tgaagtaaac
    22441 agaagacgtt aacggaagat tcaccactgg atagtccgaa gctggactgt ccattggcat
    22501 cagtaatgga tcaagtaact ccgcagccgg aatatcctgc tgttcggctt gctcaaccag
    22561 ttcacgcaga tgttccaggg tcaccatccg ttcaaccgga tatttactta ccgccagacg
    22621 acgcaagtaa ataacatgcg cgccacaacc gagtttttcg ccgagatcgt caatgatagt
    22681 gcggatataa gtgccttttg agcagtgaat ttccagctcc agctcattgc cttcatggcg
    22741 aataaacagc aattcataaa cggtaatcgg gcgcgcttca cgcggaactt caatgccctg
    22801 acgcgcgtat tcgtacagtt ttttgccctg atatttcagt gctgaataca tcgaagggat
    22861 ctgttcgata tcgccacgga aggtatccag cgccgccgcc agttgctctg cactaaaggt
    22921 taccggacgt tcttcaacga tttgtccgtc ggcatcagaa gtatcggtac gctgtccaag
    22981 acgcgcaatg acccgataac gtttgtcgga gtccagcaga tactgggaaa acttcgtcgc
    23041 ttccccgagg caaattggca acatgccggt cgccagcggg tccagcgcac cggtatgccc
    23101 ggcacggttg gcgttataaa tccgtttcac tttttgcagc gcatcgttgc tggacatccc
    23161 ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac gaggacgact
    23221 cattagtcct ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg tttgaccacg
    23281 ctggtcacca ggtttgacat gcgcatccct tcaaccagag agttgtcgta gaagaaggtc
    23341 agttccggca cgatacgcag gcgcatcgct ttccccagca ggctgcggat gaaaccagaa
    23401 gcttcctgca gcgctttgat gcccgcttta accgcgtctt catctttgtc gttgaggaac
    23461 gttacatata ctttggcata cgccaggtcg cgagacattt cgacaccgga aacggtggtc
    23521 atcatgccca gacgaggatc tttaatttca cgctgcagga tgagagcgat ctctttttgc
    23581 atttcctgcg ctacgcgctg cgggcgacca aattctttcg ccataataaa ttctcctgac
    23641 aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag gtggaagggc tgttcacgtt
    23701 ggcctgataa gacgcgccag cgtcgcatca ggcaatccat gccggatgcg gcgtaaacgc
    23761 cttatcccgc atggaaccct aaaaacctta agcgatggta cgttggattt cgatgatttc
    23821 gaatacttcg atcacatcgc cagtgcggac gtcgttgtag ttcttaacgc cgataccaca
    23881 ttccataccg ttacggactt cgttaacgtc atctttgaag cggcgcaggg actccagctc
    23941 gccttcgtag ataaccacgt tgtcgcgcag aacgcggatc gggttgtgac gtttaaccac
    24001 accttcggta accatacaac ctgcgatggc accaaatttc ggtgatttga atacgtcacg
    24061 aacttccgcc agaccgataa tctgttgttt cagttccgga gacagcatac cgctcattgc
    24121 cgctttcact tcgtcgatca ggttatagat gacggagtag taacgcagat ccaggctttc
    24181 cgcttcaatc actttacgtg cagaggcatc agcacgtacg ttgaagccaa ccaggatggc
    24241 attggacgcc gcagccaggg tagcgtcggt ttcggtgata ccacctacgc cagaaccgat
    24301 gatcttcact ttaacttcgt cagtagacag tttcagcaag gagtcggaga tagcttccac
    24361 agaaccctgt acgtctgcct tcaggacgat attcacttcg tgaacttcgc cttcggtcat
    24421 gttggcgaac atgttctcga gtttagattt ctgctgacgc gccagtttaa cttcgcggaa
    24481 tttaccctga cgatagagtg caacttcacg cgctttcttc tcgtcacgta caacggtaac
    24541 ttcatcaccc gcagccggaa cgccggacag gccgaggatt tccaccggaa tggacggacc
    24601 cgcttccagc acttcctgac ccagttcgtt gcgcatcgca cgaacacgac cgtattcgaa
    24661 gccacacaga acgatatcgc ccttgtgcag agtaccttca cgtaccagaa cggtagcaac
    24721 cggaccacga cctttatcga ggaaggattc gataaccgca ccgctcgcca tacctttacg
    24781 taccgctttc agttccagaa cttccgcctg cagcaggata gcgtccagca gctcatcgat
    24841 accagtaccc gctttcgcag acacatgtac gaactggctt tcaccgcccc actcttccgg
    24901 caggatgccg tactgggaga gttcgttctt aacgcgatcc ggatcggctt ctggtttatc
    24961 gatcttgttc actgcaacca ccaccggaac ctgcgccgct ttcgcgtgct ggattgcttc
    25021 gatggtctgc ggcattacgc cgtcgtcggc agcaacaacc aggactacga tgtccgttgc
    25081 ctgcgcacca cgagcacgca ttgaagtaaa cgcggcgtgc cccggggtgt ccaggaaggt
    25141 gatcatgccg ttttcagttt caacgtggta tgcaccaatg tgctgggtaa tgccgcccgc
    25201 ttcgccagag gccactttcg ttgaacgaat gtagtccagc agagaggttt taccgtggtc
    25261 aacgtgaccc atgatggtca caaccggcgc gcgcggttca gccgctgcac ccgtgtcacg
    25321 gtcgctcatt accgcttctt ccaactcgtt ttcacgacgc aggataactt tgtggcccat
    25381 ctcttcagca accagctgtg cggtttcctg atcgataacc tggttgatgg ttgccattgc
    25441 gcccagtttc atcatcgctt tgatgacctg agagccttta accgccatct tgttcgccag
    25501 ttcgccaacg gtgatggttt cgccgatcac aacgtcacgg ttaacggcct gagcaggctt
    25561 ctggaagcct tgctgcagcg aagaaccttt acgttttccg cccttaccgc cacgtactgc
    25621 tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt tcttcggacg
    25681 cgcagctttc gcgttacgac cacggccacg gccgccttcg acttcacgat cgctttcgtc
    25741 ttctgcctgg cgagcatgtt gagaagtagt aacgtgataa tcgctggaat cttcagtcgg
    25801 ttccgcgtta tcagtccatt tgttttcttc cgccatacga cgtgcttctt cagcaacgcg
    25861 acgtgcttct tcttcgagtt tacgacgcgc ttcttcttcg gctttacgct tgagctctgc
    25921 agcttcctgc tcacggcggg ctttttcagc ctgggcgttt ttagtcatat cgtcttgttg
    25981 attgctcact ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag cttcacgctt
    26041 agcttgttct gcggcctcac gttcagcttt ttgttgcgcc tcacgtttgg ccgattcttc
    26101 tgcctcacga cgggcttgct cttccgcttc acgctgcgct tgctcttccg cagcaaggcg
    26161 ttcagcctct tgcggatcgc gtttcacaaa ggtgcgtttc ttgcggactt cgatttgtac
    26221 cgatttgctt tttccaccgg taccaggaat gttaagggtg ctgcgtgttt tacgttgcag
    26281 cgtcaatttg tccgggcctg aatttttctg attcaggtgg tcaatcaaag tctgtttctc
    26341 ttgtgcagac acagagtcgt cagcagactt ccggatacct gcatcagcaa attgctgtac
    26401 caggcgttcc acggaggtct gtcgctctgc ggccagcgtt ttaatcgtta catctgtcat
    26461 gctgttcctt cctgctacag tttattacgc ttcgtcaccg aaccagcaaa tattacgggc
    26521 agccataatc agtgctccgg ctttttcgtc ggtcaaccct tcgatatcag ccagatcatc
    26581 aatgccctgt tcggcgagat cttccagcgt acaaacgcca cgggcggcca gtttgaatgc
    26641 caaatcacga tctatccctt caaggttcag cagatcgtca gccggtttgt tatcaccgag
    26701 gctttcttcc tgggcctgtg caatggtggc cagtgcattt ttagcacgct cgcgcagtgc
    26761 ttcaacggtc ggctcatcaa ggccttcgat ttccaacagc tctttcatcg gcacataggc
    26821 cagttcttcc agcgtcgaga agccttcttc taccagaaca gtcgcgaagt cttcgtcgat
    26881 gtcgagatat ttggtgaagg tgtcgatcgc tgcgtgcgct tccgcctgat gcttagcctg
    26941 caggtcgtca acggtcatca cgttgagttc ccaaccgctc agctgcgaag ccagacgcac
    27001 gttctgacca ttacggccaa tcgcctgcgc caggttaccg gcttcaacgg cgatatccat
    27061 ggtgtgttta tcttcatcca ccacgataga agcaacgtct gccggtgcca ttgcgttaat
    27121 cacgaactgc gccgggttat catcccacag gacgatatcg atacgctcgc cgcccagttc
    27181 agtagaaacc gcctgaacac gcgcgccacg catacctacg caagcaccta ccggatcgat
    27241 acgtttatcg ttggttttca ccgcgatttt cgcacgagaa cccggatcgc gagccgctgc
    27301 tttaatttca atcacttctt cgccgatttc cggcacttca atgcggaaca gttcgatcag
    27361 catttccggc ttggaacggg tgacgaacag ttgcgcgcca cgcgcttccg ggcgaacgga
    27421 atagagcacg ccacgaacgc ggtcgccagg acggaagttt tcacgcggca gcatatcttc
    27481 gcgcaggatc acggcttcag cgttgttgcc cagatccaga gagatgttgt cgcggtttac
    27541 ttttttcacc acgccggtga tgatttcacc ttcgtgttca cggaactgat caaccaccat
    27601 cgcacgttcg gcttcacgca ctttctgcac gataacctgt tttgccgtct gggtggtgat
    27661 acggtcaaag gtaacagact caatctgatc ttcaacgtaa tcgcccaggt tcaggctttc
    27721 atcttcataa cgtgcggctt caagggtgat ttccttggtc ggctgggtga cttcatcaac
    27781 aactaaccag cgacggaagg tgtcaaaatc accgcttttg cgatcgatct gtacacggac
    27841 gtcgatctct tgctcatatt ttttctttgt tgctgtcgcc agcgcgcttt ccaatgcttc
    27901 gaaaatcttc tcgcgaggta gcgccttttc attggatacg gcttcaacta cagccaaaat
    27961 ttctttgttc atcgcgggct tttcacctca tccagactat taaaagtggg gaaccaggtt
    28021 cgccttctgg atattactca gcgcgaacac ttcatcttta ccttcgacgg taactgtgat
    28081 catttcaccg tctaccgctt tgataacgcc ctgccattta cgacggtttt gtaccgccat
    28141 gcggagaacc agagtcacct cttctccgac gaaacgggcg tagtgttcag ccgtgaacag
    28201 tgggcgatcg agacccggtg aggagacttc caggttataa gcaacggtga tgggatcttc
    28261 aacatccagc acagcactta cctggtggct cacatcagca caatcatcaa cattgatgcc
    28321 atcttcacta tcaatataga tgcgcagtgt ggatgtgcga ccgcgaataa attcgatgcc
    28381 aaccagttca aaacccaggg cctcaactgg cgcagtaatc atctctgtta atttttgctc
    28441 taatgtggac aagcccaccc ccaagacata aaaaaagggc ctaaagccca gttattctgt
    28501 agtcagataa caaaaaaccc cgataaatcg gggctttata taactgaacc ctataaccgc
    28561 aactgcggtc tggagcactt tccagaagga ttttttcaaa tcccactacg aaggccgaag
    28621 tcttcacagt atatttgaaa aaggactcta agggaaagtg gttgcggggg ccggatttga
    28681 accgacgacc ttcgggttat gagcccgacg agctaccagg ctgctccacc ccgcgcctga
    28741 aacgtggcaa attctactcg ttttgggtaa aaaatgcaaa tactgctggg atttggtgta
    28801 ccgagacggg acgtaaaatc tgcaggcatt atagtgatcc acgccacatt ttgtcaacgt
    28861 ttattgctaa tcacgtgaat gaatatccag ttcactttca tttgttgaat acttttgcct
    28921 tctcctgctc tcccttaagc gcattatttt acaaaaaaca cactaaactc ttcctgtctc
    28981 cgataaaaga tgattaaatg aaaactcatt tattttgcat aaaaattcag tgagagcgga
    29041 aatccaggct cctcatcagt taattaagca gggtgttatt ttatgacgac gattctcaag
    29101 catctcccgg taggtcaacg tattggtatc gctttttccg gcggtctgga caccagtgcc
    29161 gcactgctgt ggatgcgaca aaagggagct gttccttatg catatactgc aaatctgggc
    29221 cagccagacg aagaggatta tgatgcgatc cctcgtcgtg ccatggaata cggcgcggag
    29281 aacgcacgtc tgatcgactg ccgcaaacaa ctggtggccg aaggtattgc cgctattcag
    29341 tgtggcgcat ttcataacac cactggcggc ctgacctatt tcaacacgac gccgctgggc
    29401 cgcgccgtga ctggcaccat gctggttgct gctatgaaag aagatggcgt gaatatctgg
    29461 ggtgacggta gcacctataa aggaaacgat atcgaacgtt tctatcgtta cggtctgctg
    29521 accaatgctg aactgcagat ttacaaaccg tggcttgata ctgactttat tgatgaactg
    29581 ggcggtcgtc atgaaatgtc tgaatttatg attgcctgcg gtttcgacta caaaatgtct
    29641 gtcgaaaaag cctactccac ggactccaac atgctcggcg caacgcatga agcgaaggat
    29701 ctggaatacc tcaactccag cgtcaaaatc gtcaacccga ttatgggcgt gaagttctgg
    29761 gatgagagcg tgaagatccc ggcagaagaa gtcacagtac gctttgagca aggtcatccg
    29821 gtggcgctga acggtaaaac ctttagcgac gacgtagaaa tgatgctgga agctaaccgc
    29881 atcggcggtc gtcacggcct gggcatgagc gaccagattg aaaaccgtat catcgaagca
    29941 aaaagccgcg gcatttatga agcccctggg atggcactgc tgcacattgc gtatgaacgt
    30001 ttgttgaccg gtattcacaa cgaagacacc attgagcagt atcacgcgca tggtcgtcag
    30061 ttgggccgtc tgctgtacca gggacgttgg tttgactccc aggcgctgat gctgcgggac
    30121 tctctgcaac gctgggttgc cagccagatc actggtgaag ttactctgga gctgcgccgc
    30181 gggaacgatt attcaatcct gaataccgtc tcagagaacc tgacctacaa gccagagcgt
    30241 ctgacgatgg aaaaaggcga ctcggtgttc tcgccagatg atcgtatcgg tcaattgacc
    30301 atgcgtaacc tggatatcac cgatacccgc gagaaacttt tcggttatgc caaaactggc
    30361 ctcctctcct cctctgccgc ttcaggcgtg ccgcaggtgg agaatctgga aaataaaggc
    30421 cagtaattcg acatcaaccc tgcccttcag gggtggggtt accttgctta tcctctgtaa
    30481 actcctttct gagcatcaca taatcgaaaa catcatggtt tgcatccaca acccgtgagt
    30541 ctcctttata gtgtgcaatc acttcctcca gcgttttcgg ttgttccggc tttgttaccc
    30601 ccatccacgc attaatcagg taattattgt aagctgtgga aaagatgttg ttttccgttg
    30661 tgcgatccac gccatcccca agaacggggc tataccagat aaacatcggg acatgatatg
    30721 cctgctggct ggcctccctg cctccatgaa aatagacgtt cttcttcgta gggtcacgtt
    30781 ccaggccgtg gtcggcaaaa tacatgaccg aggcgcggcg atcttttaat aattcaaaaa
    30841 cctgacctag caaactatct gtgtaatgaa tggagttgtc atagcaggca tcctgatcgt
    30901 cctgcggttg aaacacggcg ctggattgcg gataggcgct acaagccggt tcatggcttc
    30961 catttaaatg aagaacaatc agtttctttt gctgcgtatt ttgctgtaat gcttgcgata
    31021 aatgcggcaa caacaattca tcaaatcctc tgacataaac tgtttccatg gcgcgcatgg
    31081 cgatactggt aactgctgta ccattctgcc gaaaagcgga ttgcgagctt agccagaaag
    31141 tctgaaatcc tgcttgatta gccatattaa taatgttgtc cgggtaatta tgaatgtcat
    31201 gactcaaaac ggaatcagca gttaaagaaa ggggaaccga cagcgcagtg taaggtgcac
    31261 cgcttattgc ttgattaaac agtttgatct gttttctttg tgcttcgact tgcggtgtcg
    31321 tagagcgtgt atatccatac aaagacatat tgtcgacgcg tacagactcc cccacaatca
    31381 acacgtaggt atcaatacct gtatccctga ctgataattg aaaatacggc accgtgtttg
    31441 caattgagag taatctttga tgctctttcg ctgctaaagc aaaataattg agattgaaaa
    31501 acggtgtata ggtagcaaat cgcgacgcta gtatatatgg actgaacgct tttttatttt
    31561 ttgcatcttt ataagcaaat tgacaagcgg aaaataaact gcccgaaatg acaatcagca
    31621 ataatattcc tgtcactttt ttcgtcggca aggaaacatc atattttatt attactgcca
    31681 aaaaaagaag ggataaaaag gcaaaagcac atagataagg gatatacatc cccagcattt
    31741 tgacaacttc atccggatca ctctgcagca cactaatcgc aaatccatca ttgaaagttg
    31801 taccaaaggt acaccatgaa tagaggctaa tactcatgtc tgccgctgtc agtaacacaa
    31861 atggtgcggc aacaagcagg cgaagcggaa gggatgatat acgtttaaca accaggatgg
    31921 tcagaacgaa gaacagcaat cgctgaacca tatgcgctcc ggaagaggcg actaagttcg
    31981 taataccaaa aacaataaca caaagataca acaaccaatg agatttaaaa cttctaacaa
    32041 atttgttgaa tactgtcatc cagaaatcca agttcacaac caatataaac aattatcaac
    32101 aatgttcaat tttatatcag acatgttttt caactctgat ccatatcaga ccgaaacact
    32161 gttagttttt tctttttaaa tgatgcggcc attgttctca gtaattaaat aacattatca
    32221 atgcgtctct aaacattacg cagaacgtag ccatcagtag ttatactgac aggagcagga
    32281 tggtttatcg agaagtaaac atatccgtat tcgtaccgga actggagttg acacaataaa
    32341 gtgccaatta tgtcagcaga agggaaaagt ttataactaa ggcgtaccga taccatcgtt
    32401 tcaaagtacc agctacgagt aaagcaactg gacgagatac agatacctga caaccattcc
    32461 tcagaccagg accaaaacga aaaaagacgc ttttcagcgt ctcttttctg gaatttggta
    32521 ccgaggacgg gacttgaacc cgtaagccct attgggcact accacctcaa ggtagcgtgt
    32581 ctaccaattc caccacctcg gcacggatac tactttttag ttcgggatat cgctggtcgg
    32641 cttagccgga gctgctggct gaatttgttc ggttttcgcc ggcgcactca ggttttccca
    32701 ttcgctacct ttattggttt tgttgctatt gatgttaccc agcaccagac tgatgatgaa
    32761 gaataacgtt gccagcagcg ctgtcatgcg ggtcatgaag ttaccagaac cacttgaacc
    32821 aaacagcgta gcggaagcgc ctgctccgaa ggaggctccc atatcagcgc ctttaccttg
    32881 ctgcagcatg atcagaccaa caaggccaat tgccacaata aggaaaacta ctaaaagagc
    32941 ttcgtacata atcaacctgt tccttgcgga gttgccgcgt accaatgctt caaccaataa
    33001 agcgggagtt tttaatcttt cccactgaag cgggtgtgaa tactaaccaa agcgaatgac
    33061 cttcgcaagg gcaattttat tgcattgtat caactgcgga aaaaaacagc aaaaccgatg
    33121 tgttcgctga gaaaaaaggc gacaatcgcc gcctttttag ccagttattt atcgttttaa
    33181 acggctttta ctgcatcggc gatgcggtgt gcaaattcag tcacctgcgc ttcgtcttcg
    33241 ccttccacca tcacgcgaat taacggttcg gtgccggatt tacgcaacag cacacgtcca
    33301 cggctaccca gcgcagcttc aacttctgcg gtcacagctt tgactgactc atgctccagc
    33361 ggatcgccgc tgcctgctgt gtaacgcacg ttaaccagaa tctgcgggaa cattttcatg
    33421 ccgctgcaaa ggtcgtgcag gctcatatgg ttacgcgcca tcgccgccag cacctgcaag
    33481 ccagcaacaa tgccgtcacc ggtagtggtt ttatccagca ggatcacatg accggagttc
    33541 tctgcaccga tacgccagcc tttctcctgc attttttcca gtacgtagcg gtcacccact
    33601 ttcgcgcgcg caaatggaat acctaactgt ttcagcgcca gttcaagccc catgttgctc
    33661 atcaatgtac ccacagcacc accacgcagc tggccctgac gaagaccttc acgcgcgatg
    33721 atatacatga tctgatcgcc atcgacttta ttgccttcat ggtcaaccat aatcacgcga
    33781 tcgccgtcgc cgtcgaaggc aataccgaga tccgcttttt cagccagcac acgagcctgg
    33841 agcgcgcgaa cgtcggtagc ccccacttcg gcattgatgt ttacaccgtt cggctcacaa
    33901 ccgatagcga taacgttcgc ccccagttcg cgcagcacgt tcggcgcgat gtgataagtc
    33961 gcaccgtttg cacaatccac cacaatcttc agttcactga ggctaagttc gttcgggaac
    34021 gtggctttgc aaaactcgat atagcgaccc gcagcatcaa cgatacggct ggctttaccc
    34081 agttctgccg aatcaacgca gctgatctct ttttccattt ccgcttcgat ggcctcttct
    34141 accgcatccg gtagtttggt gccgtcgata gagaagaatt taatgccgtt atcgtagaat
    34201 gggttatgcg atgcagaaat tacaattcca gcctctgcgc ggaaggtacg cgtcagataa
    34261 gccacggccg gtgttggcat cgggccagtg aagagcgcgg aaagaccggc tgccgcaagc
    34321 cccgcttcca gtgctgactc cagcatatag ccagaaatac gcgtgtcttt accaataata
    34381 atcttacggg agccgtggcg cgccagcact ttacccgcgg cccaacccag cttaagcaca
    34441 aaatcagggg tgatcggcgc atcccctaca cgaccacgaa tcccatcggt accgaaatat
    34501 ttacgattac tcatagcgtt tgttttcctt tgcagacaga gtggcttcca ccacccgcat
    34561 cgcttctacg gtttctttga cgtcatgaac acgaatgatg tgcgcgcctt gcattgcggc
    34621 aatgaccgca caggccagac taccgctcaa acgctcggac ggccccacgt tcagcagctg
    34681 tccaatcatc gattttcgtg acatacccac caacagcggc aggttgaaat ggtgaaattc
    34741 agccaggcgc gccagtaatg aatagttatg ggagagattt ttaccgaaac cgaatccggg
    34801 gtcgagcaac aatttctctt ttgcgatacc cgcctgctcg caacgtgcta tttgctcaat
    34861 aaagtagcga ttcacttctg caaagacatc gtgatacttc ggggcttcct gcatggtttt
    34921 tggatttccc tgcatatgca tcagacaaac cggtaaaccg gtttctgcag ccgcctccag
    34981 agcgccaggt tcggaaaggg agcggatatc gttaataatg tgagcgccaa ctttcgctga
    35041 ctcacggatg acttctggtt tggatgtatc taccgagatc cagacttcga agcgttgagc
    35101 aattgcctca accacaggaa taacacgttg taactcttct tcaacgctaa cttccgccgc
    35161 ccctggacgt gtggactcgc caccaacgtc aatgatcgtc gcgccagcgt tgatcatcag
    35221 attcgcatgt ttcaccgcat ctatcagcga gttatgcgtg ccaccatccg aaaaggaatc
    35281 aggcgtgacg ttgaggatcc ccattacgtg aggatggcta aggtccagtg aagtaccctg
    35341 ggcaaagagt ttcataatgt tatccctggt atgaattgat aagaaaaaac cccggagcac
    35401 gccccggggt ttttggtaca aatacagtca tctgatgcgg gaacttactt gtcgcctaac
    35461 tgctctgaca tggtgttacc cgggttcggc gtacgcggtt catcaaccgg acgaggagcc
    35521 tttggactac cattgtcgcc agaattgtta gaagcgcctg gttcttccca gcccgctggc
    35581 ggacgtacat cgcgacgtgc catcaggtca tcaatctgcg gtgcgtcgat agtctcatat
    35641 ttcatgagag catctttcat cgcatgcagt atatccatat tgtcggtcag aagctgacgc
    35701 gcacgattat agttgcgctc aatcagtgct ttcacttcct ggtcgatgat acgtgcagtt
    35761 tcatcggaca tatgtttcgc cttcgctacg ctacggccga ggaacacttc accttcttct
    35821 tccgcgtaca gcagtggacc caatttctca gagaagcccc actgagtcac catgttacgt
    35881 gccaggttgg tcgcaacttt aatatcgttg gacgcaccgg tagaaacatg ttccggcccg
    35941 tagatgatct cttctgccag acgaccaccg tacagcgtag aaatctggct ttccagtttc
    36001 tgacggctag cgctgattgc gtcgccctca ggcaagaaga aagtcacacc cagcgcacga
    36061 ccgcgtggga taatcgtcac tttgtgcacc ggatcatgtt ccggcaccag gcgaccgata
    36121 atcgcatgac ccgcttcgtg gtaagccgtc gattctttct gcgcttccgt catcaccatg
    36181 gagcgacgtt ccgcacccat catgattttg tctttcgctt tctcgaactc aaccatcgac
    36241 acaacgcgtt tgttgccacg agcagcgaac agtgccgctt cgttcaccag gttcgccagg
    36301 tcagcaccgg agaaaccagg agtaccacgg gcaatgattg ccgcgtcgat atcgggtgcc
    36361 aatggtacgc gacgcatgtg aactttcagg atctgttcac gaccgcgaac atctggcaag
    36421 ccaaccacaa cctgacggtc gaaacggcca ggacgcagca gcgccgggtc gagaacgtcc
    36481 ggacggttag tcgcggcgat aacgatgata ccttcgttac cttcgaagcc atccatctca
    36541 accagcatct ggttcagagt ctgttcacgt tcatcgtgac caccgcccag acctgcgcca
    36601 cgctggcggc ctacggcgtc gatttcatcg ataaagatga tgcacggtgc cgctttcttc
    36661 gcctgttcga acatgtcacg aacacgggat gcacccacac cgacgaacat ttctacgaag
    36721 tcagaaccgg agatagtaaa gaacggaact ttcgcttcgc ctgcaatcgc tttcgccagc
    36781 aacgttttac cggtacccgg aggaccgacc atcaggacgc ctttcgggat cttaccgccg
    36841 agtttctgga agcggctcgg ctcgcgcaga tactcaacca gttcagcaac ttcttctttt
    36901 gcttcgtcgc agcccgcaac gtcagcaaag gtcgttttga tctgatcttc agtcagcatg
    36961 cgcgctttgc tcttaccaaa cgacatggca cctttgccac cgccgccctg catttgacgc
    37021 atgaagaaga tccagacacc aatcagcaac agcatcggga accaggagat gaagatagaa
    37081 gccagcaggc ttggttcttc aggcggttca ccgacaacct tgacgttctt ggtcaacaag
    37141 ttatccagta atttcggatc ctgaaccgga atgtaagtgg tataacggtt actatctttc
    37201 ttggtaacgt tgatttcacg tccgttgata cgcgcttcac gaacctggtc gttattgacc
    37261 tcttgtagga aggtagagta atccacctta cggccattag actcgctggg cccaaagctc
    37321 tggaatactg acatcagcac aacggcaatg accagccaga gtattaggtt tttcgccatg
    37381 tcactcaagg gattaacctc ttattacaac tgtgttaaaa acagcgtcag gatactctat
    37441 atccagcatc tttcaaactt tcgtctgaaa tctcccggtt agggtttacg cccggtcgct
    37501 acaatataca cttcccgcga acgtgcgcga gaagagtccg gcttacgaac tttgaccttc
    37561 gtaaacaggg agcgaatttc ccttagatac tcatcgaaac cttcgccctg gaacaccttc
    37621 actacaaaac tgccacctgg cgctaataca tcacgacaca tttctagcgc cagttccacc
    37681 agatacatgg cacgggggat atccaccgcc ggtgttccgc tcatgtttgg tgccatatcg
    37741 gacatgacaa cctggacttt gctgtcgcca acgcgctcca gcagtgcttt catcaccagt
    37801 tcatcacgaa aatcgccctg aagaaagtcc acaccaacga taggatccat aggtaaaaga
    37861 tcgcaagcga tgatacggcc tttaccgcca atttgggtga ccacatattg tgaccaacca
    37921 cccggcgcag caccaaggtc gacaaccgtc attcccggtt taaagagttt gtcactttgc
    37981 tgtatttcat caagtttaaa ccaggcacgg gaacgtaacc cctttttctg tgcctgttga
    38041 acatatttat cgctaaagtg ttcctgaagc cagcggctgg agctggcaga acgcttctta
    38101 cctgtcattt aactttccca ttgggataac tcatcgtaac caattgcgta aatttttacg
    38161 cgcctatttg gcgatatatg ggagatggcg gtagaatgac ccgttttcaa tcccaacgta
    38221 agcaaaaaat acgatgaatc tgagtactaa acaaaaacag cacctgaaag gtctggcaca
    38281 tccgctcaag ccagttgttc tgcttggcag taatggtttg accgaagggg tgctggccga
    38341 gattgaacaa gcgttagagc accatgaact catcaaggtg aaaatcgcca ccgaagatcg
    38401 cgaaactaaa accttgatcg tggaagctat cgtgcgcgaa accggcgcct gtaatgtaca
    38461 ggtcatcggt aaaacgctgg tgctttatcg cccaactaaa gaacgtaaaa tctcgctgcc
    38521 acgctaagat tatcctaaag ttacacacat ttgctgtgta aaacgagggg ttttccgcag
    38581 gcaggagagc aaaatgccac gctctgttcg ttgataaaag gccgcatagc ggcctttttc
    38641 ctttctttac aatacatcaa catcttgagt attgggtaat tcttacaggt attccacctt
    38701 aattacttca aattctactt cgccgcccgg cgttttgatg accacaacat catcttcttc
    38761 tttgccgatc aggccacgag caataggcga gtttacagaa atcaggtttt gtttaaagtc
    38821 agcttcgtca tcgccaacga tgcgataagt ctgttcttcg tcagaatcca gattcagcac
    38881 cgttacggta gcaccaaaaa taacgcgccc attgttgggc attttggtga catcaatcac
    38941 ctgcgcgttc gacagcttgg cttcgatgtc tttgatacgg ccttcgcaga agccctgctg
    39001 ttcacgagct gcgtggtatt cggcgttttc tttcaggtcg ccatgctcac gcgcttccgc
    39061 gatagcagca atgatttcag gacggcgcac agatttcaga aaatccagct cttcgcgtaa
    39121 tttttcagcg ccgcgtaagg tcatcggaat agcttgcatt tgttatacct cttgaatatt
    39181 cctgatgggg caagtcttca cccaccccgg ctgttcggcc tgcccggcat aactcctcac
    39241 cgggaccaga agcaaaaaaa taccgacccg ggtacaagtc ccaggtcagc tacaattcac
    39301 attttgatag tcattttacc ctgaagttcc cgaagggtca tcgtttactt tatagggcgt
    39361 tgcgccgtag tatgacggct cgattccagg ttgttagcgc gagattatgc gattttccag
    39421 atttatcatc ggattgacca gctgtatagc gttcagtgtt caggccgcaa atgttgatga
    39481 gtacattact caactccccg ctggtgccaa ccttgccctg atggtgcaaa aagtcggcgc
    39541 gtcggccccc gctattgatt accacagtca gcagatggcg ctgcccgcca gtacgcagaa
    39601 agtgattact gcactggcgg cgttgattca actcggcccc gattttcgtt ttaccacgac
    39661 gcttgaaacc aaaggcaatg tggaaaacgg cgtacttaag ggtgacttag tggcgcgatt
    39721 tggtgccgat ccgacgttaa gacgtcagga tattcgcaat atggtcgcga ctttgaaaaa
    39781 atctggcgtc aaccaaatcg atggcaatgt gttgatagat acctccattt tcgccagcca
    39841 cgataaagcc cccggctggc catggaatga catgacacaa tgctttagcg ctccgcctgc
    39901 cgccgccata gttgaccgca actgtttctc cgtctcgctc tatagtgccc caaaacctgg
    39961 tgatatggct tttatacgcg tggcatctta ttaccccgtt acgatgttca gccaggtacg
    40021 caccctcccc cgtggttctg ccgaagcgca atactgcgag ctggatgtgg tgccaggcga
    40081 cctgaaccgc tttacgctga ccggatgcct gccacaacgt tctgagccgc tcccgttggc
    40141 ttttgccgtg caggatggtg ccagctatgc tggagcaatt ctgaaagatg agttaaaaca
    40201 ggcaggtatc acctggagcg gaacactgct gcgtcagacg caggttaacg aacccggaac
    40261 ggtagttgcc agtaaacagt cggccccgtt gcacgatctg cttaagatta tgctgaaaaa
    40321 gtcggacaac atgattgccg atacggtttt ccgcatgatc ggccatgcgc gcttcaatgt
    40381 gcctggaaca tggcgggccg ggtcggacgc cgtgcgtcag atcctgcgcc agcaagccgg
    40441 tgtcgatatt ggaaatacca ttattgccga tggttcaggg ctttcgcggc ataacctgat
    40501 tgctcccgcc accatgatgc aggtgctgca atacattgcc caacacgaca atgaacttaa
    40561 ctttatctct atgctgccgc tggcgggcta tgacggctct ttgcagtacc gtgcaggtct
    40621 gcatcaggcg ggcgtggatg gaaaagtctc agcgaaaacc ggttcgttgc agggggtata
    40681 taacctggcg ggattcatta ccacagcgag cgggcaacga atggcgtttg tgcaatatct
    40741 ttctggctat gcagtagaac cggcggatca gcgtaatcgc cgtattccgt tggtacgttt
    40801 tgaaagccgt ttgtataagg atatttacca gaacaattag tctaaagaga aaccccggca
    40861 catggccggg gttttgctga ttaacgcttg taaatgaact caacgccttc ttcgtcgtct
    40921 tcgtcccagt cgtcatccca gtcttcatca tcctcttcag caatctcttc aagctgctgg
    40981 cgatgataat catcccacat gaattcgact ttctctggct gtttcgcttc ttcagcctgc
    41041 acgaccgggt tttcaatgat aaaggtcatc acatcccagc agagatcttt cacgcccagt
    41101 ccgctcgccg cagagatcag ataatattta tcttcccaac ccagcgcctc agcgatcgct
    41161 ttcgctttct cttcggcttc tgccttatcc agcaggtcga tcttgttgaa gactaaccaa
    41221 cgcggtttcg ccgccagatc ctggctgtat ttttccagct cgctgataat aatacgcgcg
    41281 ttttcaaccg gatcggtgcc atcaatcgga tcgatatcga tgaggtgcaa caggacgcgg
    41341 caacgctcca ggtgcttcag gaagcgaatg cccagacctg cgccttctgc agcgccttca
    41401 atcagtcctg gaatatcggc aacaacgaag ctcttttcgt tgtccattcg taccacacca
    41461 agacttggca ccagagtggt aaacggataa tccgccactt tcggtttagc cgccgatact
    41521 gcgcggataa atgtcgattt acccgcgttt ggcatcccca gcataccgac gtcagccagc
    41581 agcatcagct ccagtagcag ctcacgctta tcgcccggcg taccgttagt tttctgccgt
    41641 ggtgtacggt taactgacga tttgaaacgg gtattgccca gaccgtgcca gccgccctta
    41701 gcaaccagca gacgctgacc gtgtttggtc atatcgccca tggtttcacc ggtaccctgg
    41761 tcgataacac gcgtacctac cggcacttta atcgtcacgt ctttaccgcg cttaccggta
    41821 cagtcgcggc ttgcgccatt ctgaccacgc tctgcgcgga aagatttttc aaaacgataa
    41881 tcgataagcg tgttcaggtt ctcgtcggct tccatccata cgtcaccgcc gtcaccgccg
    41941 tcgccgccat ccgggccgcc tttcggaata tacttttcgc ggcggaagct cacgcaacca
    42001 ttaccgccat cacctgcaac gaccagaatc gatgcttcat caacaaactt cattttattc
    42061 tccgtaaatc attcgcctgc gcggggttgc gataccaccg ttgtatgctt acgtaatccg
    42121 ccccaaatac gatgaccaat ggcggaatac atcgcgcccg caaccacgac aaacgcaccg
    42181 agataaccta aaaggtttaa catcggtctg gcgaagaaat cgggccaggc cagtgataaa
    42241 agatctgaaa aaaacagcgt aaacagtggg gtgagcgtaa tgatcgcgct cacctgcgct
    42301 gcctgccagc gagccatcgc ttccgccagg gcgccatatc ctaccaatgt attcagtccg
    42361 caaaaaatta aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc aggctttgcc
    42421 agagggaaga gcgcaattgt acataaagtg tacagcaaaa acaggatctg cggtgaggcc
    42481 agtcgacgca ataaaacctt ttgcgccacg ccataactca cccacaccgt cgccgcaccg
    42541 accccaaaga ttactcccca ggtgtaatcg gtgagctttg taaatatctc gaccagactg
    42601 gtgttaaaaa acatcaccag gccgctcagg agcatcaatg cccctacaac ctgagtgctg
    42661 cgcattttct ctttcaggat aaatacgctg gcaaccatca tgccaactgg cgagagttgc
    42721 ccaatcacct gcgaagcggt cggacccagg tattgcaagg atgagctgaa caggatgaag
    42781 ttcccaaaca gcccggcggt cgccactgcc aacaaaatca accagcgtgg cttacgaaac
    42841 acgcgtaatg gcggcaaccg cttcttcacc gcaagaatgg cacccaggcc aatactcgcc
    42901 atcaagaaac ggtaaaacac gattgtcgga ggttccatca cctccagcac ctgcttcatt
    42961 gcgattggca acgcccccca gcaaattgct gtggtgagcg ccaaaagaat gccaatgcct
    43021 gcctgctgct tcataccgta ttccctgcaa agcgcatttg cggattaacg tcgcaccatg
    43081 cgcattttta ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg gctttcatcc
    43141 gttaccggga cgcgaaaaac ttattcagct tcgatgctga taaatttacg gtttttcggg
    43201 cctttaactt cgaatttcac tttaccgtct gctttagcaa acagagtgtg gtcacgaccg
    43261 caacctacgt tagcgccagc gtggaatttg gtaccacgtt gacgaacgat gatgctaccc
    43321 gccagaacgg attcgccacc gaaacgctta acgcccaggc gtttagcttc tgaatcgcga
    43381 ccgttacgtg tggagccgcc agccttttta tgtgccattt gaaatctctc ctcaggtctt
    43441 aggcgctgat gccagtaatt ttcacatcag tgaaccactg acgatggccc tgctgcttac
    43501 gatagtgttt acgacgacga aacttaacga ttttaacttt ctcgccacga ccgtgagcaa
    43561 caacttcagc tttgattacg ccgccatcaa cgaaaggaac gccgattttg acttcttcac
    43621 cgtttgcgat catcagcact tcagcgaact caacagtttc gccagttgcg atgtccagct
    43681 tttccaggcg aacggtctga ccttcgctta ctcggtgttg tttaccacca ctttggaaaa
    43741 ccgcgtacat ataaaactcc gcttccgcgc acaccttttc aatgattcag agtgcgctat
    43801 aaatattcac aatagggcgc gaatattacg caaaacgcac gcctttgaca agtgctacag
    43861 tcaatacacg aagaaaaaaa acacaacttg tacggtaacg tttatctgtg ccattttttc
    43921 agtacaatca ccctatattc ctaaccataa accctaagtt gcctttgttc acagtaaggt
    43981 aatcggggcg aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg agttaaccgc
    44041 gcaagatatg gcgggtgtta atgcggcaat ccttgagcag cttaattccg acgtccaact
    44101 gatcaatcag ttaggctatt acatcgtcag cggcggcggt aaacgtattc gtccgatgat
    44161 tgctgtactg gctgcacgag ctgttggcta tgagggaaat gcgcatgtca ccattgctgc
    44221 cctgatcgag tttatccaca cggcgactct gctgcacgac gacgttgtgg atgaatcaga
    44281 tatgcgcagg ggtaaagcta ccgccaacgc tgcatttggc aatgccgcca gcgtgctggt
    44341 aggcgatttt atttataccc gcgcgttcca gatgatgacc agcctcggct cgctcaaagt
    44401 gctggaagtc atgtcagaag ccgtaaacgt catcgcagaa ggtgaagttc tgcaactgat
    44461 gaacgttaac gatccggaca tcactgaaga aaactacatg cgcgttatct atagcaaaac
    44521 cgcgcgtctg tttgaggctg ccgcgcagtg ttccgggatt ctggctggct gtacgccgga
    44581 ggaggagaaa ggcctgcagg attatgggcg ctatctcggc actgctttcc agttgatcga
    44641 cgatttactc gattacaatg ccgatggcga acagttaggt aaaaacgtcg gcgacgatct
    44701 gaacgaaggt aaaccgacgc tgccgctgct gcatgcaatg catcatggca caccagaaca
    44761 ggcacagatg attcgtaccg ccatcgaaca gggtaacggt cgccatcttc tggaaccggt
    44821 tctggaagca atgaacgctt gtggatctct tgaatggacg cgtcagcgtg ccgaggaaga
    44881 agcagacaaa gccatcgcag cgttacaggt gctcccggac accccgtggc gagaagcact
    44941 catcggcctc gcgcacatcg ctgttcaacg cgatcgttaa tcccctcccc tcatcccgcg
    45001 cagcgcgcgg gatgattcca gtaaattcca taaaaacact tattcagctc tataacacct
    45061 gaaaaatagg gacgtacatc tttatatatt ctgaatattc acactcttta caggaacttt
    45121 ttagagcaat aggccatcag gagtatagtg atgctcgaca gaagaagtgt tctgaatgaa
    45181 agcgactaac ttaaggagtg aggaaagatg gaaagtaatt tcattgactg gcatcccgct
    45241 gacatcattg cgggtttgcg caagaaggga acgtcaatgg cggcggaatc tcgcagaaat
    45301 ggtttgagtt cctcaacgct ggcgaacgca ttatcgcgcc cctggccgaa aggagagatg
    45361 attattgcga aagccctggg aactgacccc tggattatct ggccatctcg ctaccatgat
    45421 ccgcagaccc atgagtttat cgacagaacg cagttgatgc gtagctacac taaaccgaaa
    45481 aaatgagtgg tctggcggta gccccgcgaa cggggctgcc agctctcaga caattactcg
    45541 cctttcacac gctcaatatt tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag
    45601 ccacgatcga tgtgataaat acgatcaact accgtcgtcc cttccgcaat acagccagcc
    45661 agcaccaggc ttgctgatgc acgcagatcg gttgccataa cctgtgcgcc agaaagtttt
    45721 tcaacaccgt gacaaataac ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc
    45781 agctctggca catgcataaa gcggttttca aagaccgttt cggtgatgaa gccggtccct
    45841 tctgccacca ggttcaacag cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc
    45901 ggcgcggtac gtacgttaac agccttcgga cgtttgccat gcatatccag gctaatccag
    45961 tcttcgccga cttcgatgtc tgctccagcg tcacgcagtt tcgccagcac ggcgtcgaga
    46021 gtatctggct gcgcgttacg gcagataatt ttgccgcgag agatcgccgc cgccaccagg
    46081 aaagtaccgg tttcgatacg atccggcagc acgcgataga caccgccgcc taaacgttcc
    46141 acaccttcga tgacgatacg atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc
    46201 aggaagttcg cggtatcgac gatttccggt tcacgcgctg cgttttcaat aatcgtggtg
    46261 ccttccgcca gggttgcagc acacatgatg gtcaccgttg cgccaacgct gactttatcc
    46321 atcacgatat gtgcgccttt cagacgacca tcgacggaag ctttaacgta accttcttcc
    46381 agtttgatgg tcgcgcctaa ttgttcgaga ccagaaatgt gtagatcaac cggacgtgca
    46441 ccaatcgtac aaccgccagg cagtgaaact tgcccctgac caaagcgcgc taccagcggc
    46501 cccagcgccc agatagaagc acgcatggtt ttaaccagat cgtaaggtgc gcagaataca
    46561 ttaacgtcgc gggcatcaat atgcacagaa ccattacgtt ctactttcgc acccagctgg
    46621 cttagcagct tcattgatgt atcgacgtct tttagtttcg ggacgttctg gatctctacc
    46681 ggttcttccg ccagtagtgc ggcaaaaagg ataggcagag cagcattttt agcgccggaa
    46741 attgtgactt cgccctggag cttcgttggc ccctgaacac gaaatttatc catttagttt
    46801 gttctcagtt aacaattcat atccgctacc ggcgaatcgc ccatagctca aaagccgttc
    46861 agtttgcgat cgcgcgccca ctccgcaggg gtatacgctt tgatcgacac agcatgaatg
    46921 cggttatccg caatatattc catcagcggg ccatagaccg tctgctgttt tttaacccga
    46981 ctcatgccgt caaacaactc acccacggca ataacctgaa agtggctgcc atcgccggaa
    47041 acgtggactt cctggaggga gagagcgttc atcaacacgc tctgaatttc attattttcc
    47101 atgggatctt caatcatcag ttaataaacc agcgaaacat cttagagcaa agttgcgctg
    47161 gcataaataa gcaaaaagcc tcgctgataa atcagacaag gctcgacttg caggcaggtt
    47221 tgccggacag gcggttaacg ccatatccgg cctgaaaaaa tttaacgagg cagaacatca
    47281 gcaggcaaat tatacaattt cgccagggta tacactttgt cgtttacccc ctgaagcgtc
    47341 acattgttgc cctgcttttt cgccagatcg ataagatgga gcagcagcgc cagtccacct
    47401 gtatcaacgc gggatacacg gctaagatcg atacaggtaa tccccttcac cgcttcctca
    47461 cgcatttccc aaagcggtag cagaacgtcc tgatccagct ctccggataa cgccagcgtg
    47521 tcacccgtct gcatccagct cagtgactcg ctcattattt tttctcttcc agagtgattt
    47581 tctgttgaga aatcgatttc agttgcgcag tcaggccgtc aatacctttg gtacgcagca
    47641 gcgttcccca ctcgttttgt ttggtggtga tcatactgac gccttcagca atcatgtcgt
    47701 aagcctgcca gttgcccgtc tgggagtttt tacgccactg gaagtccaga cgcaccggcg
    47761 ggcggccatt cgggtcaata atggtaacgc gaataggcac aatggtttta tcacccagcg
    47821 gctgttctgg cgcaatctga taggtttgac cgtgatacat cgccagcgcc tgaccgtaag
    47881 cctgcttcag gtactcacgg aaagcggcaa agtaagcatc acgttgagct ggggtcgcac
    47941 tcttgtaata ctgacccagc accagcgcac cggcgtattt cacctgtacg tatggcagca
    48001 gttcctgatc aacaatggta cgcagataat ccgggttggc ccgaatttgt ggttgctcat
    48061 tcttcaggcg atcgaacgtt ttctgcgccg cctcgtccat cagcttatac ggattggtct
    48121 ggtctgccgc ggttgccgca ctcagcggtg caatcaccag caaagcgacc atcattaaac
    48181 gtttaaacat gcgtcggttc tcctgaaatt atttcgttgt acccacaggt tcagtggttt
    48241 cattattacc tggcgcagca gctggcgcat cgccactatt cttattgtca tcgcctttac
    48301 taccgtaaag gaactgacca ataagatctt ccagcaccat cgcagacttg gtgtcctgaa
    48361 ttgtatcgcc atccttcagg atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta
    48421 atgccagata ttgttccccc agcaggccgg aagtacgaat gctcagcgaa ctggtatcag
    48481 gaatgtggtt ataacgttgt tcaatttcca gcgttacgcg cggcagatag gttttcgggt
    48541 ccagcgtaat atccgccacc cggcccacaa caacaccacc aatgctgacc ggagagcgcg
    48601 ctttcaggcc gccaatgtta tcgaacgtcg cataaagcgt gtaggtcggt tcagtacgta
    48661 tggacgtcac gttcgccgcc ttcaggcaaa caaacagcgc cgccagtagt gctgctaata
    48721 aaaagatacc cacccaaatt tcattttttt tcgtttgcat gaactcaatt cccaaacatc
    48781 aatgcggtca gcacaaaatc cagccccaga acagccagag acgagtggac aacggtgcga
    48841 gtggttgccc ggctaatccc ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa
    48901 atccacgtca ccgtgatggc aaacaccacg ctcttaatca gacagttgac cagatccata
    48961 cgccagtcga cggcattttg cattgccgac cagaagaacc cgctatcaat gcctttccaa
    49021 ctgacgccga ccagagatcc gccccagatc cccacggcga cgaaaataac cgtcaacagt
    49081 ggtaatgaaa taaccccagc ccagaaacgg ggagaaataa cccgacgcag cggatccacc
    49141 gccatcatct ccatactgga gagttgctct gtagcgcgca tcaggccgat ttctgcggtt
    49201 agcgccgaac cagcacgccc ggcaaacaac aacgcggcaa ccaccggccc cagttcacgc
    49261 agtagcgata acgccaccag catacccaga ctggtttccg cactataagt ggtcagaacc
    49321 agataacctt gcagccccaa caccattccg atgaacacgc cagaaaccac aataatcagc
    49381 atcgacagga cgccgacatt atagagctgg cgcaccagca gcggcgcatg tttgcgaaat
    49441 tccggtttgc cgaccagcgc attgaataac attaacccgg cccgcccgaa cgttctcagg
    49501 gttttaatcc ctttatgtcc gagcgacgcc agcgcattta acagcatgag tggcttaact
    49561 ccctggtaaa agatcagcgt gataatcgcc ggcaggatag cggaacggaa caggcccgtc
    49621 agctatcccg tccagaaact gacgtacgcg tggatcagga ttcgcctgca acgcctgggc
    49681 actgccatga gcgacaattt ttttgtccgc caggatccag gcgtgatcgg caatacttaa
    49741 cacttccggc acatcgtgag aaaccaccac acaagtcaca cccagcgcgc tgttcagctc
    49801 agaaatcagc ttcaccagta cgcccatggt gatgggatct tgcccaacaa agggttcatc
    49861 aaacatgatg agatccggct ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat
    49921 cccaccggaa agttcagaag gcattagttt agccgctcca cgcagcccca cggcctccag
    49981 cttcatcatc accgtactat gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg
    50041 ataggcgacg ttgtcaaata cgttcatatc agtgaacaac gcccctgact gaaataacat
    50101 gctcatccgt ttgcgcactg tatacaggcg cgaacgagac atcgccggaa tattctcacc
    50161 atcgaaaaga atctcaccat gatctggtgc gatttgcccg ccaatcagac ggagtagcgt
    50221 cgttttaccg atgcccgatg gccccatgat cgccgtgatc ttccctcgcg gcacggtcag
    50281 ggaaatatta tcgaagatgc agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac
    50341 taaattcgcc acagactgct ccataattca cccttcgtct tgcgttgatt ttctaagcat
    50401 ggcgctcaat ttaaccttga acccaacata tttacagaat attacccgcc gtggttagcg
    50461 aaagctggca tttgttttac tttttagccg cataaagtca aaattaagaa tccgttacgg
    50521 ctttctgaaa atcttcagcg gaccggcgag tatacctgaa gaaaggacgt tagatgcttt
    50581 tagctacggc actgttaatt gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg
    50641 tttttgccgc gtctattctt tgccgaacct ttggcatccc gccgctaatc atcggcatga
    50701 cggtggtcag tattggtaca tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc
    50761 acgaacaacg cgatttagcc gttggtacag ccctcggctc aaacattatc aatatattgc
    50821 tgatcctcgg tctggctgcg ctggttcgtc cttttaccgt ccattctgat gttctacgcc
    50881 gtgaattacc cttaatgttg ttggtcagcg tggtggccgg ttccgtactc tatgacggac
    50941 aacttagtcg aagcgatggt atctttctcc tctttctggc tgtgctatgg ctgctgttca
    51001 ttgttaaact tgcacgtcag gctgaacgtc aggggactga cagcctgacc agagagcagc
    51061 ttgcagagct gcctcgtgac ggcggattgc ccgtcgcgtt tttatggctc ggcattgcgc
    51121 ttatcatcat gccagtggcc acgcggatgg tggttgataa cgccacggtg ctggcgaatt
    51181 actttgccat cagcgagttg acgatgggtc tgacggcaat tgctatcgga accagcctgc
    51241 cggaactggc aaccgcaata gcgggggttc gcaaaggtga aaacgacatt gctgtcggaa
    51301 atatcattgg cgcaaacatt tttaatattg tcatcgtatt gggtttaccc gcgctgataa
    51361 cgccaggaga gattgatcca ctggcgtaca gtcgtgacta cagcgtgatg ttgctggtga
    51421 gcattatttt tgcgttgctg tgctggcggc gctccccgca accgggccgt ggtgtagggg
    51481 tattattaac tggcggattt atcgtatggc tggcgatgtt gtactggtta tcgccaatac
    51541 tcgttgaata actggaaacg cattatgtcg cacgtagagt tacaaccggg ttttgacttt
    51601 cagcaagcag gtaaagaagt cctggcgatt gaacgtgaat gcctggcgga gcttgatcaa
    51661 tacatcaatc agaatttcac gcttgcctgt gaaaagatgt tctggtgtaa agggaaagtt
    51721 gtcgtcatgg ggatggggaa atcggggcac atcgggcgca aaatggccgc aacgtttgcc
    51781 agcaccggta caccttcatt tttcgtccat cctggtgaag ccgcgcatgg tgatttaggc
    51841 atggtcaccc cacaggatgt ggtgattgct atctctaact caggtgaatc cagcgaaatc
    51901 acggccttaa ttccagtgct taagcgtctt cacgtaccgt taatctgcat caccggtcgc
    51961 ccggagagca gcatggcgcg cgccgcagat gtgcatctgt gtgttaaagt agcgaaagaa
    52021 gcctgtccgt tagggctggc accgaccagc agcaccaccg ccacgctggt tatgggcgat
    52081 gccctcgctg tcgcgctgtt aaaagcacgc ggctttactg ctgaagattt tgcgctctca
    52141 cacccaggcg gcgcactggg tcgtaaactt ctgctgcgcg taaacgatat tatgcatacg
    52201 ggcgatgaga tcccgcatgt taagaaaacg gccagtctgc gtgacgcatt gctggaagtt
    52261 acccgcaaaa atcttggtgt gactgtcatt tgcgatgaca atatgatgat tgaaggcatc
    52321 tttaccgacg gtgatttacg ccgtgtcttc gatatgggcg tggatgttcg tcagttaagt
    52381 attgccgatg tgatgacgcc ggggggaata cgtgtgcgcc ctggcattct ggccgttgag
    52441 gcactgaact taatgcagtc ccgccatatc acctccgtga tggttgccga tggcgaccat
    52501 ttactcggtg tgttacatat gcatgattta ctgcgtgcag gcgtagtgta aagattcaag
    52561 gataaacaac aatgagcaaa gcaggtgcgt cgcttgcgac ctgttacggc cctgtcagcg
    52621 ccgacgttat agcaaaagca gagaacattc gtctgctgat cctcgatgtc gatggcgtac
    52681 tgtcagatgg cctgatttat atgggcaata atggcgaaga gctgaaagcg ttcaatgttc
    52741 gtgacggtta tggcattcgt tgtgcgctca cctctgatat tgaagtcgct atcattaccg
    52801 ggcgaaaggc taaactggta gaagatcgtt gtgccacatt ggggatcact cacttgtatc
    52861 aggggcagtc aaacaaactg atcgccttta gcgatttgct ggaaaaactg gcgattgccc
    52921 cggaaaatgt ggcttatgtc ggcgatgatc tcatcgactg gccggtaatg gaaaaagtgg
    52981 gtttaagcat cgccgtggcc gatgcgcatc cactgttaat cccgcgcgcc gattacgtga
    53041 cgcgcattgc tggcggtcgt ggtgcagtgc gtgaagtttg cgacttatta ctcctggcgc
    53101 agggcaaact ggatgaagcc aaagggcaat cgatatgagt aaagccagac gttgggttat
    53161 cattgtgctg tcactggcgg ttctggtgat gatcggcatt aatatggccg aaaaagacga
    53221 taccgcccag gtggtcgtca acaacaatga tcccacctat aaaagcgagc atacggacac
    53281 gctcgtctat aacccagaag gggcactaag ctatcgattg attgctcaac acgttgaata
    53341 ttattccgat caggccgttt cgtggtttac gcagccggta cttaccacgt ttgataagga
    53401 taaaatcccg acatggtccg taaaagcaga taaagccaag ctgaccaatg accggatgct
    53461 ctatttatat ggacacgttg aagtcaacgc actcgtgccg gactctcaac ttcgcagaat
    53521 cacgacggat aacgcgcaga tcaatctggt gacgcaggat gttacctctg aagacctcgt
    53581 cacgttatac ggaacaacat ttaactccag cggtctgaaa atgcgcggca acttacgcag
    53641 caagaacgcc gagctgattg aaaaggttag aacatcctat gaaattcaaa acaaacaaac
    53701 tcagccttaa tcttgtgctt gccagctcac ttctggccgc cagcattccg gcatttgccg
    53761 taaccggaga cactgatcag ccgatccaca ttgaatcgga ccagcaatct cttgatatgc
    53821 aaggcaacgt ggttaccttt accggcaatg tcatcgtcac ccagggcacc atcaaaatta
    53881 atgccgacaa agtggtcgtt acccgtccgg gcggcgaaca aggtaaagaa gtgattgacg
    53941 gctacggtaa accggcaacg ttctaccaga tgcaggacaa cggtaaaccc gttgaaggtc
    54001 acgcttcaca gatgcactac gaactggcaa aagattttgt cgttctgacg ggtaatgctt
    54061 atctgcagca ggtcgatagc aacattaagg gcgataagat cacttacctg gtgaaagagc
    54121 agaaaatgca ggctttcagc gacaaaggca agcgcgtaac caccgttctg gtgccgtcgc
    54181 agctgcagga caaaaacaat aaaggccaga ccccggcaca gaagaagggt aattaattcg
    54241 ttatggcaac attaactgca aagaaccttg caaaagccta taaaggccgt cgcgtggtag
    54301 aagacgtcag cctgaccgtc aactccgggg aaattgtcgg tctgctgggg ccaaacggtg
    54361 ccggtaagac caccactttc tacatggttg ttggcattgt gccgcgcgat gcgggcaaca
    54421 tcattattga tgatgacgat atcagtctgc tgcctctgca tgcacgcgcg cgccgcggta
    54481 tcggctatct gccgcaggaa gcctccattt tccgtcgcct cagcgtttac gataacctga
    54541 tggcggtact gcaaattcgt gacgacttgt ctgctgaaca acgtgaagac cgcgcgaacg
    54601 agctgatgga agagtttcac attgagcacc tgcgtgacag catggggcag tcactctccg
    54661 ggggtgaacg tcgccgtgta gaaattgccc gtgcactggc tgcgaatccg aaatttattc
    54721 tgctcgacga accgtttgcc ggggttgacc cgatctcggt tatcgacatt aaacgcatca
    54781 ttgagcacct gcgcgacagc ggcctgggcg tactgatcac tgaccacaac gtgcgtgaaa
    54841 cactggcggt ttgtgaacgc gcttatatcg tcagtcaggg gcatttgatc gcccacggca
    54901 cgcctacaga aatcttacaa gacgaacacg ttaagcgtgt ataccttggg gaagacttca
    54961 gactctgata gggtagaagt ttgcgatgtt ttagcaggag agtacgattc tgaacatgaa
    55021 gcaaggtttg caactcaggc ttagccaaca actggcgatg acgccacagc tccaacaggc
    55081 aattcgtctg ttgcagttgt cgacgctgga acttcagcag gagctacagc aggcgctgga
    55141 gagtaatccg ctgcttgagc aaatcgacac tcatgaagaa atcgacaccc gcgaaacgca
    55201 agacagtgaa acactggaca ccgccgacgc gctcgaacaa aaagagatgc cggaagagct
    55261 gccgctcgat gccagttggg acaccattta caccgctggt acaccatccg gcaccagcgg
    55321 tgactacatt gacgacgagc tgccggtcta tcagggcgaa acgacgcaga ccttgcagga
    55381 ttacctgatg tggcaggtcg agctgacacc gttttccgac actgaccgcg ctattgctac
    55441 ctctatcgtc gatgccgttg atgacaccgg ttatctgact gtcccgctgg aagatattct
    55501 cgaaagtatg ggcgatgaag agattgacat cgacgaagtt gaagccgtcc ttaagcggat
    55561 ccaacggttt gatccggtcg gtgtggcggc aaaagatctg cgtgactgtc tgctgatcca
    55621 actctcccaa ttcgataaaa ccacgccatg gctggaagag gccagactga tcattagcga
    55681 tcatctcgat ctgttagcca atcacgactt ccgcacttta atgcgcgtca cgcggctgaa
    55741 agaagatgtg ctgaaagaag ccgtcaatct gatccagtcg ctcgatccgc gccccgggca
    55801 gtcgatccag acgggcgaac ctgagtatgt cattccagat gtgctggtgc gtaagcataa
    55861 cggtcactgg acggtagaac tcaacagtga cagcattccg cgtctgcaaa tcaaccagca
    55921 ctacgcctcg atgtgcaata acgcgcgcaa cgatggtgac agccagttta tccgcagcaa
    55981 tctgcaggat gccaaatggt tgatcaagag cctggaaagc cgtaacgata cgctactgcg
    56041 cgtgagtcgc tgtatcgttg aacagcagca agccttcttt gagcaaggcg aagaatatat
    56101 gaaaccgatg gtactggccg atatcgccca ggctgtcgaa atgcatgaat cgacgatatc
    56161 tcgcgtgacc acgcaaaaat acctgcatag tccacgaggc atttttgaac tgaagtattt
    56221 cttttccagt cacgtcaata ccgagggcgg cggcgaagct tcctccacgg cgattcgtgc
    56281 gctggtgaag aaattaatcg cggcggaaaa cccagcgaaa ccgttgagcg acagcaagtt
    56341 aacctctttg ctgtcggaac aaggtatcat ggtggcacgc cgcactgttg cgaagtaccg
    56401 agagtcttta tccattccgc cgtcaaacca gcgtaaacag ctcgtttgac ccaaccgata
    56461 aggaagacac tatgcagctc aacattaccg gaaataacgt cgagatcacc gaggcactgc
    56521 gcgaatttgt tacagccaaa tttgccaaac ttgagcaata ttttgaccga atcaaccagg
    56581 tctatgttgt tctgaaagtg gagaaagtca cccacacctc agatgcaaca ctgcatgtaa
    56641 acggtggtga aattcatgcc agcgcggaag gtcaggacat gtacgctgcc attgatggct
    56701 taattgataa gctggcacgc cagttgacca aacataaaga taaactgaaa caacactaat
    56761 tgtccgggca attagcatgt gcatggcggc ctgttgtgcg gcacaacggg ccatttgtac
    56821 ggttaatgct ccgagcctgt tccactgttt gagtgggcag gttcttaggt gaaattatga
    56881 caaataatga tacaactcta cagcttagca gtgttcttaa cagggaatgt acgcgaagcc
    56941 gcgtccactg tcagagcaaa aaacgcgccc tggaaatcat cagcgagctg gcggcgaaac
    57001 aacttagtct gccgcctcag gtggtttttg aagctatcct gacgcgggaa aaaatgggca
    57061 gtaccggtat cggcaatggt attgccattc cgcatggcaa actggaagaa gatactctgc
    57121 gcgccgttgg cgttttcgtt cagctcgaaa cgcctatagc tttcgatgcc attgacaacc
    57181 aaccggtaga tttactattt gcgttgctgg ttccggctga ccaaaccaaa acgcacctgc
    57241 atactctgtc gctggtggcg aaacgtctgg cggacaaaac catctgccgc cgtttacgcg
    57301 cagcccagag cgatgaagag ctgtatcaaa tcattacgga taccgaaggt actccggatg
    57361 aagcgtagtt attcggtaat gtctctttta gacgttgtga ggagaaacag tacatggtac
    57421 tgatgatcgt cagcggacgt tcaggttcag gtaaatctgt cgccctgcgt gcgctggaag
    57481 atatgggttt ttactgcgtg gataaccttc ccgtagtgtt gttacccgat ctggctcgaa
    57541 ccctggccga tcgcgagatt tctgccgccg tcagcattga tgttcgtaat atgccggagt
    57601 caccagaaat attcgaacag gcgatgagta acctgcctga cgctttctca ccgcaactcc
    57661 tgttcctgga tgccgaccgt aataccttaa ttcgtcgtta cagtgacacg cgccgactgc
    57721 atccgctttc cagcaaaaac ctgtcgctgg aaagtgctat cgacaaagaa agcgatttgc
    57781 tggagcctct gcgttcgcga gcggatctga ttgtcgatac ctcagaaatg tccgttcacg
    57841 agctggcaga aatgctgcgt acccgtctgc tgggtaaacg tgaacgtgaa ctgaccatgg
    57901 tctttgagtc tttcggcttc aaacacggta tccctatcga tgcagattac gtctttgacg
    57961 tgcgcttctt gccgaacccg cactgggatc cgaaactgcg tccaatgaca ggtcttgata
    58021 aacctgtcgc cgcgttcctc gaccgccaca cagaagtaca caattttatc taccagacgc
    58081 gaagctatct tgagctatgg ttacctatgc tggaaaccaa caaccgtagc tacctgacgg
    58141 tcgccattgg ttgtaccggc gggaagcacc gttcggtgta tattgcagag caactggcag
    58201 actacttccg ctcgcgcggt aaaaacgtcc agtcacgcca tcgtacgctg gaaaaacgta
    58261 aaccatgacc gtcaagcaaa ctgttgaaat cacaaacaag ctgggcatgc atgcccggcc
    58321 tgcaatgaag ctgtttgaat taatgcaagg ttttgacgca gaagtgctgt tacgtaatga
    58381 cgaaggtacc gaggccgaag ccaacagcgt tattgcactg ctgatgctgg attctgccaa
    58441 aggacggcag attgaagttg aagcgaccgg tccacaggaa gaggaagcac tggccgccgt
    58501 tatcgccctc tttaattctg gttttgatga agattaatct tcatcacttt gacattcaaa
    58561 tcccttcaag ctcccccgac aataagaaaa gcgcgtgctg aaatcgttct caatcaacgt
    58621 catttgtaca ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac cttaagaagc
    58681 attgtttagg tttcgtttaa gttaatcgac catactggag atcgtcagaa aatatttcca
    58741 ggagatggca tgattcgctt atctgaacaa agtcccctgg gcaccggaag acatcggaaa
    58801 tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga ttgtctacca tcgtggcgat
    58861 ggtggcgata aagagatccg ccgtgagtta aagtactacg cgcatcttgg tcgccggtta
    58921 aaagactgga gtggaatacc gcgctatcac ggtaccgttg agacggattg cggaaccggt
    58981 tatgtctacg atgtgattgc agattttgac ggcaaaccct ccattacgct gaccgaattt
    59041 gctgaacaat gccgttacga agaagacatt gcacaactgc gccagctact gaaacagctg
    59101 aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca gaatatcctc
    59161 tgtcaccgca tcagtgagtc agaggttatc cccgtcgtct gcgacaacat tggtgaaagc
    59221 acattgattc cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca agagcggtta
    59281 tggaaacgat ttatcgccca accggcgctg gctattgccc tgcagaaaga tctgcaacct
    59341 cgtgagagca aaacgctggc cctcacttcg cgcgaagctt aatccagctg gtgctgttgc
    59401 ataaacgact caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg acggctacgc
    59461 acgtagcccg atggcgcgga gactttgaaa cgaagtggat taggtaatac agcagccagt
    59521 aatgcagctt ccgagcgggt aagtttgctc gcgggtttgt ggaaataacg ttgtgccgca
    59581 gcttcgacgc caaacacacc gtcgccaaat tcggcgatat tcaggtaaac cgtcaggata
    59641 cgctttttgc tccagactgt ttctatcccc agcgttaatc cagcttccag cccttttcgt
    59701 acccagctac gcccatccca taaaaagaga tttttggccg tctgctgaga aatcgttgaa
    59761 gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca gggctttctc aatggaagcg
    59821 acatcaaagc cccagtgctc aggaaatttc tgatcttctg cggcaatcac cgccagtccc
    59881 atccacggcg agatttgatc catactgacc cagtcagaat gtgccacgta acgaaaattg
    59941 ccatgcagcc aggcgctgac ctgtcgctcg accattaccg ctgagaaggg aacaggcgca
    60001 acactaaaca acgcgatgcc cccgccccag aaaacggcga ggacaaccat taaccgcaaa
    60061 agaaaacggc gaacgaaact aaacaccgtt aagcggcttt tactcattca gccagaacca
    60121 gcacgcggga aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc tgcgccagca
    60181 tatatgctgg ggtggtgaca atcttattgt cttcatcaac cacgatatca tcgacaggac
    60241 atggcacatg ctccgcgccc atctcttcca gcacttctgc ggtatcgata tcagtaccga
    60301 tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat cgccggggca atacacataa
    60361 aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag cgcctttaat tcacggtcaa
    60421 cggtgcattc gctaccaaga ctggcaaaat tgcttaaatt cttcgccgcg ccaaaacccc
    60481 ccggcacaat caacgcatcc agttcagcgg catcggcctg ggccagagga cggatttcac
    60541 cgcgcgttat tcgtgccgct tcaatcagca cattgcgcgt ttccgtcatc gcttcgcccg
    60601 ttaaatggtt gataacatca acctgctgct tatccggtgc aaagcagacc gcctgcgcac
    60661 cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg aatttcagaa ccgtcataga
    60721 cgccgcatcc gctcagaatt acgccaattt tcttcattgt gatcatcctt tcgcaattga
    60781 ctgaaacaca ttaatttttt taataaaaat ggtacgcatc acacatttaa ctgattcatg
    60841 taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac agtgctgtgc
    60901 ccttgtaact catcataata atttacggcg cagccaagat ttccctggtg ttggcgcagt
    60961 attcgcgcac cccggtctag ccggggtcat tttttagtgg cttttgccac ccacgctttc
    61021 agcacttcta cgtcgtgacg ccactcttct ttcatctctt caatccattc accgacgtta
    61081 tcttcccagg caggaaggtc aggagactga atttgctgac ccagctgttg cagatggcgt
    61141 aaccccactg accccgccgc acctttaatt ttatgtcctt cctcaacaat gcctttttta
    61201 tcctgcgccg tcagattcga ctccagcacg ctgacatagc ccggcatcat cttctcaaac
    61261 accgctaacc cgtcggtgat cagcttcggt cctacaagtg cgagatactg ttccagcatg
    61321 ggaatatcga gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt actctcctca
    61381 tcatcctggg tatcccagaa tttcttgatc atcgcggtta gcgccggaac cgaaagcggc
    61441 ttactcagca catcatccat tccagcattg aggtactctt gtttgtcttt cagcacgtta
    61501 gcggttaagg ccaccagcgg cggtaaatcc tcgcgcggat aacgcttcgt cagttcacga
    61561 gagatatcca gcccggtcat atctggcaac tgaatatcca gcaacaccag gtcgtattcg
    61621 cccggtttaa acatctccag cgccgccttg ccggtcatgg cgacatcaac gctgttacct
    61681 aatttttcca gcacagaacg cgcaacaatc acgttcagtt caatgtcttc caccagcagc
    61741 acattcagcg ccggtaaagg catatcgtct tcatcaaacg catcatcgac ctcttctgct
    61801 accgacggtg cgtggatcgt caacgtaaag gttgaacctt tgccctgttc gctggtaacc
    61861 gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa cggccagacc aataccggtg
    61921 ccggtggcag gtttaccgcc atgactgtct ttcacctggt aatacatggc gaaaatttta
    61981 tccagctcat cctgcggaat gccgatacca gagtcttcca cttcaaaatg cagcatatcg
    62041 ccttcatcgt agcgcacgcg cacggtaacc tggccttgct gggtgaattt gacggcgtta
    62101 ctgatgagat tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac ctgatgcggt
    62161 aatggcagcg taggctccag gttaaagcgc aatccttttt gttgcgcctg caatgcggaa
    62221 agattttcca gatcggcaag gaagctggtg aaatcaaccg gctgattatc aagctggacc
    62281 ttacgccgtt ccatcttatc catgtcgata atatcgttga agatattccc gagcgtgacg
    62341 gccgaaacat ggatggtttt gagatatttt tcctgctcgg cggtgagttc ggtatccagc
    62401 agaatgcggc tcaggccgac gataccattc agcggtgtac gcaattcgtg actgatggtg
    62461 gagataaacg tcgttttgtc gcggctggcc cgttcaagcg catcctgata ccgcttacgc
    62521 tcggtaatgt cgcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg gtcgtagtac
    62581 ggcactttac ggatttcaaa gcaggctttg cgtccgtcgg gataatccag ccactgctca
    62641 taggtcagtg acacattatg acggaacact ttttcatcgg tttcaatgac tttcgcagcg
    62701 gcttccggtg agtaaacatc agcaggtttc agatgaacca gttgtttttc gctttttccg
    62761 gtcagcagct ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt acgataaaaa
    62821 accaggtcgg gtgaagcgtc aaggaaggaa cgtaagaatg aggattgctg ctcgagctga
    62881 atttgtgtct cttcgcgctc tttgatttca attttcagtt ggccgaaggt ttcctgcagt
    62941 tctgcttccg ctttttcacg aacggcgatt tcctgattta gctgggcaat attatctttt
    63001 aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg caccagtcgt
    63061 gacagacgtt gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa gtagaccgcc
    63121 cacggcgtaa tcagcaaacc aaagaagata gaacgaataa catcaatgct ttcgacctga
    63181 ccatgcagca ccatggttac cgccatttgt accacaatgg caagaacgac gagggccagc
    63241 gccagtaaca ttgagaagcg caccagacct aacttcatca tcaggtcaac ataatactgc
    63301 gccagcagac gaatttgctt cattagggaa ttccttcacg acaacctgcc ccaataatac
    63361 ccaattctga cagttacgtt ataaattgtg tgagaaatgc ggaattcccc tcaatggcct
    63421 acgccgttgg aggtagccac ggacgtccca gcgccgatcc ctgtacgcca tgttcattca
    63481 gataacgatc cagctcgacc attcccgtcc atcgattttc gcaccacagc ggagcaagca
    63541 gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat cacttccggt ggcgtatggc
    63601 gaatcatttc tccggcagtg agcgtgtaat cctccagttc aattccgttc aaacgccccg
    63661 cttcccaggc tttcgccata atactgcctt tcacaatatg cagcggatgc agctttatgc
    63721 cgtccacgcc ggtttcaacc actcgctcca gcgtttgcaa acattccgtc tgcccttcgc
    63781 caggcaggcc gacaattaag tgggaacaca ccttcagccc gcgctgacgt gccagctggg
    63841 ttgtacgctg ataacaggca aaatcatggc cgcggttgat gcgatgcagc gttttgtcgt
    63901 gggcggtttg tagccccagc tccagccaca cttcgtaacc ctggtcctta tattcgcaaa
    63961 gcagatccag caccgcatcc ggcacgcagt ccgggcgggt accaacacac aaaccgacaa
    64021 tattggcctg gctcaccgcc tgctgataca tagaacgcag cacctgaact tccgcaaagg
    64081 tgctggtata cgcctgaaag taggccagat agcgtttagc gcggttaact aaattcgcct
    64141 ggtgcgccag ttgctcggca atggaacgat gctgctgcgc ttcatcggca aacgaggcca
    64201 cattacagaa tgtgcagcca ccacgcccga tggtaccgtc acggttaggg cagctaaaac
    64261 cgccgtgcag cgtcggctta tgcacctttt gcccataacg acgggtgaga tcaccaccaa
    64321 acatattgac taatttctgt aactgcataa tctgatagac cgcgccttga aaagaggcca
    64381 aagcctgcca tttttagccc aattcggcga tgacctggat caatcgtccc ggcccgcttt
    64441 tatcaactgc ataatcaatc aaaattaccg aaatttcatg cacaatcaca taaatcactt
    64501 ttgcttatct tgtgtcagat ttttttatct ccttatggat tttaggcaaa aacagtagca
    64561 tgaaacgtta ttaccaatta aggcagtata aaatgctggt tttctcgtca gttcaaggca
    64621 ggataagggt taacacgcct ttatgacagt caggaattgg ctgtttctct aacgacgccc
    64681 cttttagcct taaatagaaa atccacttta atttcagcca attaataaag aattttgcgc
    64741 taaagcacat ttctgtacca ataagcttgc catttgacct gtatcagctt tcccgataag
    64801 ttggaaatcc gctggaagct ttctggatga gcagcctgct catcatattt atgcagtaat
    64861 tgagatcccc tcttcaccgt attaaccgat gcgaaaagga caacaagggg gcgaatgcga
    64921 ggcgcgcgta tgacacgcaa accccgtcgc cacgctcttt ctgtgcccgt gcgcagcggt
    64981 tcggaagtgg ggttcccgca gagcctgggg gaggttcacg atatgttgta cgataaatcc
    65041 cttgagaggg ataactgtgg tttcggcctg atcgcccaca tagaaggcga acctagccac
    65101 aaggtagtgc gtactgcaat acacgcactg gcccgcatgc agcaccgtgg cgcgattctc
    65161 gccgatggta aaaccggtga cggttgcggc ttgctgttac aaaaaccgga tcgctttttt
    65221 cgcatcgttg cgcaggagcg cggctggcgt ttagcaaaaa actacgccgt cgggatgctc
    65281 ttcctgaata aagatcctga actcgccgct gccgcacgcc gcatcgttga agaagagctg
    65341 caacgcgaaa cgttgtcgat tgtgggctgg cgtgatgtcc ccactaacga aggcgtgctg
    65401 ggtgaaatcg ccctctcctc tctgccacgc attgagcaaa tttttgtgaa cgccccggca
    65461 ggctggcgtc cgcgcgatat ggagcgccgt ctgtttatcg cccgccgccg cattgaaaag
    65521 cgtctcgaag ccgacaaaga cttctacgtc tgtagcctgt cgaatctggt caatatttat
    65581 aaaggtctgt gtatgccggc ggatctgccg cgcttctacc tggacctcgc ggacctgcgt
    65641 ctggaatcgg ctatctgcct gtttcaccag cgcttttcca ctaacaccgt accgcgctgg
    65701 ccgctggcgc agccgttccg ctacctcgcg cataacggtg aaatcaacac catcaccggt
    65761 aaccgccaat gggcgcgtgc gcgtacctat aaattccaga cgccgctgat ccctgacctg
    65821 cacgacgccg cgccgttcgt caacgaaacc ggctctgact ccagttcgat ggataacatg
    65881 ctggaactcc tgctggcagg cgggatggat atcatccgtg ccatgcgtct gttagtgcca
    65941 ccagcctggc agaacaaccc ggatatggac ccggaactgc gtgccttctt tgactttaac
    66001 tccatgcata tggagccgtg ggatggtccg gcagggatcg tgatgtccga tggccgtttc
    66061 gccgcctgta acctcgaccg taacggtctg cgtccggcac gctacgtcat caccaaagat
    66121 aaactgatca cctgcgcctc tgaagtcggt atctgggatt atcagcctga cgaagtggtc
    66181 gaaaaaggcc gcgtagggcc aggcgaactg atggtgatcg acactcgcag tgggcgcatt
    66241 ctacactcgg cagaaaccga tgatgacctg aaaagccgcc atccctataa agagtggatg
    66301 gagaaaaacg tccgccgtct ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc
    66361 cgcgaactgg acgacgacac gcttgccagc taccagaaac aatttaacta cagcgcggaa
    66421 gagctggatt cggtgatccg cgtactgggt gaaaacggtc aggaagcggt cggttcgatg
    66481 ggcgacgata ccccattcgc cgtgctctcc agccagccgc gcattattta cgactacttc
    66541 cgccagcagt ttgctcaggt aaccaacccg ccaatcgacc cgctgcgcga agcgcatgtt
    66601 atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct tctgtgaagc agaaggccag
    66661 gcgcaccgtt taagctttaa atcgccgatt ctgctctatt ccgatttcaa acagctcacg
    66721 acgatgaaag aggatcacta ccgcgcagat acgctggata tcacctttga cgtcactaaa
    66781 accacgctcg aagcgacggt gaaagagctg tgcgacaaag ccgaaaaaat ggtacgtagt
    66841 ggcaccgtgc tgctggtgct ctccgaccgg aatatcgcta aagatcgcct gccggttccg
    66901 gctccgatgg cggttggcgc gatccagacc cgtcttgtcg agcaaagcct gcgttgcgat
    66961 gccaacatca tcgtagaaac cgccagcgcc cgtgatccgc accacttcgc cgtgttgctg
    67021 ggtttcggcg cgacggctat ttatccatac cttgcctatg aaacgctggg ccgcctggta
    67081 gacacccacg cgattgccaa agattatcgt accgtgatgc tcaactatcg taacggcatc
    67141 aacaaaggct tgtacaaaat catgtccaaa atgggcatct ccaccatcgc ctcttaccgc
    67201 tgctcgaaac tgtttgaagc ggtcggtctg cacgatgatg tcgtgggcct gtgcttccag
    67261 ggggcggtta gccgcattgg gggggctagc tttgaagact tccagcagga tctgctgaat
    67321 ctgtcgaaac gagcctggct ggcgcgtaag cctatcagcc agggcggcct gctgaaatac
    67381 gtgcacggtg gcgaatacca cgcctacaat ccggacgtgg tgcgcacgct gcaacaggcg
    67441 gtacagagcg gcgagtatag cgactatcag gaatacgcga agctggttaa tgagcgtccg
    67501 gcaaccacgc tacgcgatct gctggcaatt acgccgggtg aaaacgcggt caacattgct
    67561 gatgttgaac cggcaagcga actgtttaaa cgcttcgata ccgccgcgat gtctatcggc
    67621 gcgttaagcc cggaagccca cgaggcgctg gcggaagcga tgaacagcat cggcggtaac
    67681 tcaaactccg gcgaaggcgg tgaagacccg gcgcgctatg gcactaacaa agtgtcgcgc
    67741 atcaagcagg tggcgtccgg tcgcttcggc gttactccgg cgtacctggt caatgccgac
    67801 gtcattcaga taaaagtcgc ccagggcgcg aagccgggtg aaggaggtca gttaccgggt
    67861 gataaagtca ctccttacat tgccaaactg cgctattcgg tgccgggcgt gacgctgatc
    67921 tccccgccgc cgcaccacga tatctactct attgaggatt tagcgcagct gattttcgac
    67981 ctcaagcagg ttaacccgaa agcgatgatc tccgtgaagc tggtttccga accgggcgta
    68041 ggcaccatcg cgactggcgt ggcaaaagct tatgccgatt taatcaccat cgcaggctat
    68101 gacggcggca ccggcgcaag tccgctttca tcggtgaaat acgcaggctg tccgtgggag
    68161 ctggggcttg ttgaaaccca acaggcgctg gttgccaacg gcttgcgtca caagatccgt
    68221 ttgcaggtcg atggcggcct gaaaacgggt gtcgatatca tcaaagcagc gattcttggc
    68281 gcggaaagtt ttggcttcgg cactggcccg atggtagcgc ttggctgtaa atatctgcga
    68341 atttgccacc tgaacaactg cgccacgggt gtagcaactc aggatgacaa actgcgtaag
    68401 aatcactatc acggcctgcc gttcaaagtg actaattact ttgagtttat cgcccgtgaa
    68461 acccgcgagc tgatggcaca gctgggggta acgcgtctgg tggatctgat tggtcgcacc
    68521 gacctgctga aagagctgga cggtttcacc gccaaacagc agaaactggc actgtcgaag
    68581 ctgctggaga ctgctgagcc gcatgctggt aaagcgctct actgcaccga aaacaacccg
    68641 ccgtttgata acggcctgct gaacgcgcag ttgctgcaac aggcaaaacc gtttgtcgat
    68701 gaacgccaga gcaaaacctt ctggttcgat atccgcaaca ccgaccgttc tgtcggcgca
    68761 tcgctttcag gctatatcgc ccagacgcac ggcgatcagg ggctggctgc cgatcctatc
    68821 aaagcgtact tcaacggcac cgcaggccag agcttcggcg tgtggaacgc gggcggcgtg
    68881 gaactgtacc tgaccggtga tgccaacgac tatgtcggta aaggcatggc gggcggctta
    68941 atcgccattc gtcctccggt tggctcagcc ttccgcagcc atgaagcgag cattatcggc
    69001 aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg ccgcaggccg cgcgggtgaa
    69061 cgtttcggcg tgcgtaactc cggtgctatc accgtggtag aaggcattgg cgacaacggc
    69121 tgtgaatata tgacgggtgg tatcgtctgt attctgggta aaaccggcgt taacttcggc
    69181 gcaggcatga ctggcggctt cgcttacgtt ctcgatgaaa gcggcgattt ccgcaaacgc
    69241 gttaacccgg aactggtcga ggtcttaagc gttgacgatc tggcgatcca tgaagagcat
    69301 ctgcgcggtc ttatcaccga gcatgttcag cataccggtt ctcagcgcgg tgaagagatt
    69361 ctggcgaact ggtcaacctt cgccactaaa tttgcgctgg ttaaaccgaa gtccagtgat
    69421 gtaaaagcac tgctgggtca ccgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag
    69481 taaggggtag caacaatgag tcagaatgtt tatcaattta tcgacctgca gcgcgttgat
    69541 ccgcctaaga aaccgctgaa gatccgcaaa attgagtttg ttgaaattta cgagccgttt
    69601 tccgaaggcc aggccaaagc gcaggctgac cgctgcctgt cgtgcggcaa cccatactgc
    69661 gagtggaaat gcccggtaca caactacatc ccgaactggc tgaagctcgc caacgagggg
    69721 cgtatttttg aagcggcgga actgtcgcac cagaccaaca ccctgccgga agtatgcgga
    69781 cgtgtctgcc cgcaagaccg tctgtgcgaa ggttcctgca ccctgaacga tgagtttggc
    69841 gctgtgacca tcggcaacat tgagcgttat atcaacgata aagcgttcga aatgggctgg
    69901 cgtccggatc tgtccggcgt gaaacagacc ggtaaaaaag tggcgattat cggcgctggc
    69961 ccggcgggtc tggcgtgtgc ggatgttctg acgcgcaacg gcgtaaaagc ggtcgtcttc
    70021 gaccgtcacc cggaaattgg cggattgctg accttcggta ttccggcttt caagctggaa
    70081 aaagaggtaa tgacgcgccg ccgtgaaatc ttcaccggca tgggtattga attcaaactc
    70141 aataccgaag tgggccgcga cgtgcagctg gacgatctgc tgagtgatta cgatgccgtg
    70201 ttccttggcg tcgggactta tcagtcaatg cgcggcgggc tggaaaacga agacgccgat
    70261 ggcgtgtacg cagcgctgcc gttccttatc gccaacacca aacagttaat gggctttggt
    70321 gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtggt ccttggcggt
    70381 ggcgacactg cgatggactg cgtgcgcacg tctgtacgcc agggggcaaa gcacgttacc
    70441 tgtgcctatc gtcgtgatga agagaacatg ccgggttccc gccgcgaagt gaaaaacgcg
    70501 cgggaagaag gcgtagagtt caaattcaac gtccagccgc tgggtataga agtgaatggc
    70561 aacggcaaag tcagcggcgt aaaaatggtg cgcacagaaa tgggcgaacc ggatgccaaa
    70621 ggccgtcgcc gcgcggagat tgtggcaggt tccgaacata tcgttccggc agatgcggtg
    70681 atcatggcgt ttggtttccg tccacacaac atggaatggc tggcaaaaca cagcgtcgag
    70741 ctggattcgc aggggcgcat tatcgccccg gaagacaacg acaacgcttt ccagaccagc
    70801 aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt ccgatctggt ggtgaccgct
    70861 attgccgaag gtcgtaaggc ggcagacggt attatgaact ggctggaagt ttaagcctgt
    70921 cccagcgccg cctcctcaga aaagagtcgg cgctgaaatt ctcctttctt tctggaaagc
    70981 gcctcgcata atctgaagaa aaccaatgta atacgctcat ctggattgtt accggtagat
    71041 gcccggcgta tagctgattc cattattcaa cgcagcgcca tataaaggga cagataatgg
    71101 aatcgctctc agagagaacc tcaacaggct accagcaaat ccacgacggt attattcacc
    71161 tggtcgatag cgcccggacg gaaacggtac gtagcgttaa cgcgctgatg accgcgacat
    71221 actgggaaat tggccgacga attgtcgaat ttgaacaagg tggcgaggcc agggctgcgt
    71281 atggtgcgca gctaatcaag cgactatcaa aggatttaag tctaaggtat aagcgtgggt
    71341 tctctgcaaa aaacttacgc caaatgaggc ttttttacct cttttttcaa catgttgaaa
    71401 ttcgccagac agtgtctggc gaattaacac cattgggaat tccgcagaca ccatctgcag
    71461 aattcccatc ggcaaagatt tggcagacgc tgtctgccaa atctttcccc ctgccctggt
    71521 ccacctacgt ccgtttactt tccgttaaaa accctgatgc ccgcagcttt tatgaaaaag
    71581 agacgctccg ctgtggttgg tctgtccgcc agctagagcg gcaaattacg acccaatttt
    71641 atgagcgaac actactgtca catgacaaat cagccatgct gcaacaacac gctcctgccg
    71701 agatgcatat tcttccgcaa caggcgatac gcgatccctt tgtgctcgaa tttctggagt
    71761 tgaaagatga atactcagaa tctgattttg aggaggcgct gatcaaccac ctgatggatt
    71821 tcatgctgga acttggggat gattttgcct ttgttggtcg gcagcgaagg ttacgcattg
    71881 atgacaactg gtttcgggtc gatctgctgt ttttccaccg ccgtttacgc tgcctgctaa
    71941 tcgtcgatct aaaagtgggc aaattcagct atagcgatgc cggacagatg aatatgtatc
    72001 tcaactacgc caaagagcac tggacactac cggatgaaaa tccgcccatc ggtctggttc
    72061 tctgtgcaga gaaaggagcc ggcgaagcgc attatgcact ggctggtttg cctaacaccg
    72121 ttctggcgag tgaatataag atgcaactac ctgatgaaaa acgactcgcg gatgaactcg
    72181 ttcgaacaca ggcggtgtta gaggaaggct atagacgccg ttaatttcag cggtttcgaa
    72241 ttctccagac actgtctgga gaatctttaa aacgaactaa gttttaagcc attaaatcag
    72301 ccttaacctt caccacaacc tttttaatct caccaggttc gccgacaacg catcctggtt
    72361 tatgcggttc tcccggcata aacacggcga acattcccgg ttttaagatg atggcttgct
    72421 cgttctcaat ggcgctgcaa agctggtaat catcctcatg gtggaactct tcacactgac
    72481 gcgcagtgcc tgccgtgcca aacagaatcc gttcctcacc gtttaataac agctggatat
    72541 caatgtattg ctcgtgcagc tccgcttttt tctcaacggg cgattgagtg ttaaacgtca
    72601 tgacattcat aaagatattg tcgcactgta attcgtaacg acccggtgct ttttcttgcg
    72661 gcctggcggc taaggctagt gttaacgcgt cctgtaacgc aggatgcaat ccggcagacg
    72721 gtaacgactg tacttcaccc attatcataa tttttctccc tgggccaaca acgcagcccc
    72781 aagtaaacct gcatcgtggc ggtagtgcgc cgccagtaaa tcaacatgaa atgccgctgg
    72841 ctcctgcgcc agatacgttt ccaccaacgc cagataccct tctgccagac caacgctgcc
    72901 accgaccact acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgccag
    72961 cacatgtgcg gagcagtgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt
    73021 gaaaatagtt ctcgcatccg cgcccgccag ttcaccctgc gctgccgctg caataccgcg
    73081 accagaagca atcgcttcca cacaaccagt gcgtccacag ccgcagactg gaccatgagg
    73141 atcagccagc gtatgcccga tgtgccccgc cagaccgcca gggccggtga gcagtttgcc
    73201 gccgctcact acaccgccgc caacaccagt ggaaacggtg ataaacacca tctcggttat
    73261 atcgccttcc agcgcctgat actccgccca cgctgcggcc tgcgcgtcgt taatggcaat
    73321 ggtcggcaaa tcggtaagtt gttccaaagt tttgactaac ggaaagtgta gcaatccacc
    73381 gagattatgc ggattcagcg ccagcaggct gccgtcgcga ataattccgg ttgaagcgat
    73441 agcaacccgc tgcgcatgaa cttgcaacgg agagactaat gcggataagg catcatgcaa
    73501 ggcttctggt gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccatc
    73561 agcgccaatc agcgcggcgg caagtttagt accgccgata tcaatcgcca gtgtggtcat
    73621 agcaccgcct ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg
    73681 gcagaaccga ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc
    73741 gtgttgtaac gcccttcggc aatcacccga catccggcgt cgctcaacgt tttcaccagc
    73801 gccagatccg gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata
    73861 atttcggctc ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc
    73921 gccagtaaac cgtgatggtg aatacgtgcc agcagcgttt caacaggcac cggacgcggt
    73981 cggtcggtgc cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcaacatct
    74041 tcaatatagg ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aatcccgata
    74101 attggcacgc tcaccaccgc acgcgtggct tgcagatttg ccacaccttc aatgcgaatg
    74161 gcaaccgcgc ccgcctgttc tgccgctaat gccatggcgg cgacgatttc gggtttatcg
    74221 agcgggctgt ccggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt
    74281 tgatccagtt gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacagaga
    74341 ccgggcaaca ggattaactt ttggttttga ctaaatcgtt tttggcgcgg ccaaatggca
    74401 cggcaccgct aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca
    74461 gccaacgctg aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca
    74521 gactgaacga gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc
    74581 cgatgattgg ggccagtgca ccgcccaatg cgccgacgtt gtaggtaaag cccaggcccg
    74641 ctgcacgctg gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc
    74701 cttgtccaag catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc
    74761 caatcgcaaa taccggaata atcagcagct gcgaggccag cagactacaa acgtacgctt
    74821 tgcgggttcc cagccagtca ccgaggaagc caccgacgca gcaccccacc gccgcgccaa
    74881 agccactaaa gaacagcaca ttggcgacag tatgcgggtc ataagccaga tcggttttca
    74941 gatacgttgg cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca
    75001 ccatcagcat tacgcccgtt ggccagcgtt tgcccgtact ctgcaccata aagctgataa
    75061 agattgcggc gcataacagc ccgagtacag cgacgattgc ggcattttgc aggttaccgg
    75121 cgaagcagaa ccacagcgca gtagccgccg ccagcgtcat tacgatattg gcaatgcgat
    75181 gttcaccacg gtaaagaatg tctaccatcg tgcgtactgg tgctttaccc ccgtgtttct
    75241 ctttccagtc ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca
    75301 aaatgccgat aaagaacagc gctctccagc cccagaccgg aaccaccaga ctatagacct
    75361 gagcggcaac gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac
    75421 gcagatgttt tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca
    75481 tccccatgcc gatgaccaga cgcgcgataa acatggtgat gtagcctggc gcaaagccgc
    75541 aggccagcgt cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt
    75601 agcggtcacc catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg
    75661 agatcagact tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta
    75721 ccagggcgat taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa
    75781 atgcgcgcca ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac
    75841 tcattgtgag tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt
    75901 acagatgcgt ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg
    75961 cgctcttgca tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc
    76021 ggtccaaacg gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt
    76081 ttcaggccgc ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt
    76141 tgcagtttct gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat
    76201 cgccagccca tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg
    76261 gaagcgaaga tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc
    76321 tgttccatct gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta
    76381 accagggtgt tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac
    76441 accaccatcg gcaaaccatc cgccgaatca ataattgccc gatagtgatc gcagtgttct
    76501 tcaaagctga aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt
    76561 ttagccgatg ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg
    76621 agtttaatct tacctttcgc ctcttcagcg acgatttcca gtacctgttc acgctcggaa
    76681 aggctttgta caaaggcttc gccggtagaa ccacccacgt ataaaccgtc gatgccctgc
    76741 tgaatattga actgaaccag gcgacgcaga ctcgctttat ccagtgcttg ttgttgatca
    76801 aaaggagtca ggagtgcggc cattacgcca cgtaaattcg ttgccataaa tacctctgaa
    76861 gtgatgctta tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa
    76921 caccccatac agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt
    76981 gttggtggtc tgaccgaagg cgtgccaggt agcagagacg ctgttgagat gcgattgcaa
    77041 cgcacgatcg gcttcatcgg gatcgtggcg gcggatcgcg tcaacgatcg caatatgctg
    77101 ttgatagcta acgttgttat gctcgtgcag agcctgatca gccacggttg gacgtgcggc
    77161 gatcagccag tcgagcaagg caacgtggat cgccatgaag attgggttac cggggatctc
    77221 cgccagcacg cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga
    77281 ctgactgttg atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc
    77341 agccgcatag cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat
    77401 cccgccagga tgagaaagga aatctttcgc catgccggaa agctcgccga tgatggtgtc
    77461 cgcagaagga cgtgagacgc gagcgcgttc gccgttgttt atctgcacca gacctttgcg
    77521 ttttaacgct gccagcgctt cgcgcaccga aggacgcccg acgttaaaga acgccatcag
    77581 ttcgcgttca gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc
    77641 cagctcttct tccaccattt cggagagttt tttacgcgcc agcgggcggc tgcgtaagtt
    77701 gcggccaatt gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat
    77761 tctgtaaggt cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg
    77821 gggaaatggc ggggttttct ctttgtgcct catcattact ataattaact gaataattaa
    77881 ctattgcgaa aaattaa
//
LOCUS       DAZKGF010000003        77273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZKGF010000003 DAZKGF010000000
VERSION     DAZKGF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.003"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(7..387)
                     /gene="panD"
                     /locus_tag="ACHOQV_000175"
     CDS             complement(7..387)
                     /gene="panD"
                     /locus_tag="ACHOQV_000175"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFP9628608.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            661..1617
                     /locus_tag="ACHOQV_000176"
     CDS             661..1617
                     /locus_tag="ACHOQV_000176"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP9628609.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGRQEERQEVRQEVRQEVRQEVRQEV
                     IQEVRQEVRQEYALRFLSKGMSREDVAEMANLPLAEVDKLIN"
     gene            1686..1901
                     /locus_tag="ACHOQV_000177"
     CDS             1686..1901
                     /locus_tag="ACHOQV_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9628610.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGFQKGYKEGLQKVRLAQRLL
                     SKGMSREDVAEVTALSLAEIDKLINSN"
     gene            complement(1983..2834)
                     /gene="panC"
                     /locus_tag="ACHOQV_000178"
     CDS             complement(1983..2834)
                     /gene="panC"
                     /locus_tag="ACHOQV_000178"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFP9628611.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2846..3640)
                     /gene="panB"
                     /locus_tag="ACHOQV_000179"
     CDS             complement(2846..3640)
                     /gene="panB"
                     /locus_tag="ACHOQV_000179"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFP9628612.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(3752..5041)
                     /locus_tag="ACHOQV_000180"
     CDS             complement(3752..5041)
                     /locus_tag="ACHOQV_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFP9628613.1"
                     /translation="MQHDNGVKMKIFFRYFLFLALCSCCYTASAGTDDNVSYLVGNDY
                     GVGPGDQKWRETGPSADITVKFRYSTSTNNLVFYKPTQLGPTGVSLYWSQLNSASGGG
                     YLYCNRSNTASGEPMAIENQMVDSGKRYGGHKLFETSVPGLYYTLKISNIWSSYTTTN
                     ISSSGMYIGDSPTQYFRWIPESEATLYWSCNNANSSNKYWAVGGVWQSLTVEFYTDTN
                     FDPTKTNKVTLSKPGGYLYSFKGYGLGGGINDHSYFLKIDFDLDNMTLSNPTCFTAAL
                     TGPSVSGSTVKMGDYTPAQIKNGATAVPFDITLQNCIRVRNIETKLMSNKVGSVSKEL
                     LANTLTGNNAAKGVGVLIEGLKNTKSAQMVLKPNDSTSVYKDYETEDDTTGGIYPDKG
                     NGTSQPLHFQATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            complement(5067..5663)
                     /locus_tag="ACHOQV_000181"
     CDS             complement(5067..5663)
                     /locus_tag="ACHOQV_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP9628614.1"
                     /translation="MRPTQRNLMKKSILLLSLLFSTTFPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHIDF
                     RALSGVMATGSSQIFANEDNTGAKNVGVVIFSIQDPANTFNVLGVTGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPALNVTSGQLISHVLVDIYYE"
     gene            complement(5690..6298)
                     /gene="yadL"
                     /locus_tag="ACHOQV_000182"
     CDS             complement(5690..6298)
                     /gene="yadL"
                     /locus_tag="ACHOQV_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFP9628615.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDIEMG
                     TCTSTLTDDGTAKLSTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTPR
                     ALCEGNSNNGPGFANASTATAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAG
                     ATGGDVVDYPMSAVLVVAKDKTVTDVTAGDFTAPATFTVTYN"
     gene            complement(6310..6882)
                     /locus_tag="ACHOQV_000183"
     CDS             complement(6310..6882)
                     /locus_tag="ACHOQV_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9628616.1"
                     /translation="MIKTTPHKIAILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     SLNGSNVVDNGNDSYTLIIPKMGLDKIVNKASQSEANFKLVASGCSSGISWIDTTMTG
                     NQSGSTSLIIPQSSDSSSTTSNIGMGFKRLETSGDTFLKPNSAEYIRWKTTEISTSGL
                     EMTVALRETTPGQGIPGKFRAQATFNFIYQ"
     gene            complement(6899..9499)
                     /gene="htrE"
                     /locus_tag="ACHOQV_000184"
     CDS             complement(6899..9499)
                     /gene="htrE"
                     /locus_tag="ACHOQV_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFP9628617.1"
                     /translation="MTIKSTKKYHNHLTRIATFCALLYSNSALCAELVEYDHTFLMGK
                     DASNIDLSRYTEGNPTLPGIYDVSVYVNDQPIMSQSIAFTVIEGKKNAQACITQKNLL
                     QFHISSPDKNSEKAILLKRDEDLGDCLNLAEMIPQSSIRYDVNDQRLDIDVPQAWIMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSESPGRTNDSIYAAFNGGINLGAWRLRASG
                     NYNWMTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPVLASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAFAIDDLSPSGYGSDLIVT
                     IEEADGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSIQDEPNLFQASYYYGLNN
                     YLTGYTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVSIPDDKTYQGQSYRISWN
                     KLFENTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYSNSASWGSYSISAQRSLN
                     EDGQTDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDFKGNNQLNISSSGYSDTN
                     RISYSVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASASSDNSRQFSLNTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAIMNIVRSDGKNIPFAADI
                     YDEQNNIIGNVGQGGQAFVRGIGQEGNIRITWIEEGKPVSCFAHYQQNTTSEKIAQSI
                     ILNGLRCQIQ"
     gene            complement(9534..10274)
                     /gene="yadV"
                     /locus_tag="ACHOQV_000185"
     CDS             complement(9534..10274)
                     /gene="yadV"
                     /locus_tag="ACHOQV_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP9628618.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGIPSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDVKMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            complement(10383..10973)
                     /locus_tag="ACHOQV_000186"
     CDS             complement(10383..10973)
                     /locus_tag="ACHOQV_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9628619.1"
                     /translation="MSKKLGFALSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFSNSKGTLNNDMSINNPSDGVNIALHNIEGSTIKQVQVNNPGDVYSKTLDATS
                     KTATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
     gene            complement(11335..11814)
                     /gene="folK"
                     /locus_tag="ACHOQV_000187"
     CDS             complement(11335..11814)
                     /gene="folK"
                     /locus_tag="ACHOQV_000187"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFP9628620.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELLFPDGLSLVEALQAKGFNELDKW"
     gene            complement(11811..13208)
                     /gene="pcnB"
                     /locus_tag="ACHOQV_000188"
     CDS             complement(11811..13208)
                     /gene="pcnB"
                     /locus_tag="ACHOQV_000188"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFP9628621.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQREQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(13268..14164)
                     /gene="gluQRS"
                     /locus_tag="ACHOQV_000189"
     CDS             complement(13268..14164)
                     /gene="gluQRS"
                     /locus_tag="ACHOQV_000189"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFP9628622.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTLFTDQLRGIIHANEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNTQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(14231..14686)
                     /gene="dksA"
                     /locus_tag="ACHOQV_000190"
     CDS             complement(14231..14686)
                     /gene="dksA"
                     /locus_tag="ACHOQV_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFP9628623.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(14864..15568)
                     /gene="sfsA"
                     /locus_tag="ACHOQV_000191"
     CDS             complement(14864..15568)
                     /gene="sfsA"
                     /locus_tag="ACHOQV_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP9628624.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTSC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(15583..16113)
                     /gene="thpR"
                     /locus_tag="ACHOQV_000192"
     CDS             complement(15583..16113)
                     /gene="thpR"
                     /locus_tag="ACHOQV_000192"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFP9628625.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            16187..18616
                     /gene="hrpB"
                     /locus_tag="ACHOQV_000193"
     CDS             16187..18616
                     /gene="hrpB"
                     /locus_tag="ACHOQV_000193"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFP9628626.1"
                     /translation="MSSLPVAAVLPELLAALDCASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVAHFDPRTGLTRLVTQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDTAQMSWLDQPPTVNLQAAKRL
                     LQMLGALEGERLSAQGQKMAVLGSDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIACRRGQDG
                     RYQLANGMGAMLDADDALSRYEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLFAAKWLPEYDWPAVDDESLLATLETWLLPHMSGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDKPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            18710..21241
                     /gene="mrcB"
                     /locus_tag="ACHOQV_000194"
     CDS             18710..21241
                     /gene="mrcB"
                     /locus_tag="ACHOQV_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFP9628627.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDYDDYDDYEDEEP
                     MPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLP
                     AAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDF
                     PDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRS
                     GFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLF
                     LSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYF
                     GRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDM
                     LSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAE
                     KAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIG
                     SLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVD
                     ALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQAF
                     QTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTG
                     RQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMS
                     IYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEM
                     QQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            21461..23650
                     /gene="fhuA"
                     /locus_tag="ACHOQV_000195"
     CDS             21461..23650
                     /gene="fhuA"
                     /locus_tag="ACHOQV_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFP9628628.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIASDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            23701..24498
                     /gene="fhuC"
                     /locus_tag="ACHOQV_000196"
     CDS             23701..24498
                     /gene="fhuC"
                     /locus_tag="ACHOQV_000196"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFP9628629.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            24498..25388
                     /gene="fhuD"
                     /locus_tag="ACHOQV_000197"
     CDS             24498..25388
                     /gene="fhuD"
                     /locus_tag="ACHOQV_000197"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFP9628630.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAIIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMIW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            25385..27367
                     /gene="fhuB"
                     /locus_tag="ACHOQV_000198"
                     /pseudo
     CDS             25385..27367
                     /gene="fhuB"
                     /locus_tag="ACHOQV_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
     gene            complement(27402..28682)
                     /gene="hemL"
                     /locus_tag="ACHOQV_000199"
     CDS             complement(27402..28682)
                     /gene="hemL"
                     /locus_tag="ACHOQV_000199"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFP9628631.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPHEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            28848..28895
                     /gene="yadX"
                     /locus_tag="ACHOQV_000200"
     CDS             28848..28895
                     /gene="yadX"
                     /locus_tag="ACHOQV_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFP9628632.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            28907..30328
                     /gene="clcA"
                     /locus_tag="ACHOQV_000201"
     CDS             28907..30328
                     /gene="clcA"
                     /locus_tag="ACHOQV_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFP9628633.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            30352..30417
                     /gene="yadW"
                     /locus_tag="ACHOQV_000202"
     CDS             30352..30417
                     /gene="yadW"
                     /locus_tag="ACHOQV_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFP9628634.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            30410..30754
                     /gene="erpA"
                     /locus_tag="ACHOQV_000203"
     CDS             30410..30754
                     /gene="erpA"
                     /locus_tag="ACHOQV_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFP9628635.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(30801..31424)
                     /gene="yadS"
                     /locus_tag="ACHOQV_000204"
     CDS             complement(30801..31424)
                     /gene="yadS"
                     /locus_tag="ACHOQV_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFP9628636.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(31462..32262)
                     /gene="btuF"
                     /locus_tag="ACHOQV_000205"
     CDS             complement(31462..32262)
                     /gene="btuF"
                     /locus_tag="ACHOQV_000205"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFP9628637.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLVPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARAPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(32255..32953)
                     /gene="mtnN"
                     /locus_tag="ACHOQV_000206"
     CDS             complement(32255..32953)
                     /gene="mtnN"
                     /locus_tag="ACHOQV_000206"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP9628638.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            33037..34554
                     /gene="dgt"
                     /locus_tag="ACHOQV_000207"
     CDS             33037..34554
                     /gene="dgt"
                     /locus_tag="ACHOQV_000207"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFP9628639.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLYSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFNKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            34684..36108
                     /gene="degP"
                     /locus_tag="ACHOQV_000208"
     CDS             34684..36108
                     /gene="degP"
                     /locus_tag="ACHOQV_000208"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFP9628640.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            36263..37420
                     /gene="cdaR"
                     /locus_tag="ACHOQV_000209"
     CDS             36263..37420
                     /gene="cdaR"
                     /locus_tag="ACHOQV_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFP9628641.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEQR"
     gene            complement(37535..37921)
                     /gene="yaeH"
                     /locus_tag="ACHOQV_000210"
     CDS             complement(37535..37921)
                     /gene="yaeH"
                     /locus_tag="ACHOQV_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFP9628642.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGIGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(38079..38891)
                     /gene="yaeI"
                     /locus_tag="ACHOQV_000211"
     CDS             complement(38079..38891)
                     /gene="yaeI"
                     /locus_tag="ACHOQV_000211"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFP9628643.1"
                     /translation="MITRRRFLQAAAATLAAGSSLSYMHYWEPGWLELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGVAQKPDLILLGGDYVLFDMQLNFSAFS
                     DVLSSLAECAPTFACYGNHDRPVGTEKNRLIGEALKSAGITVLFNEAMLITTQKQQFE
                     LVGTGDLWTGQCKPPPASEANLPRLVLAHNPDSKEVMRNEKWDLMLCGHTHGGQLRIP
                     LVGEPFAPIEDKRYAAGLNAFGERQIYTTRGVGSLYGLRLNCRPEVTILELV"
     gene            complement(38944..39768)
                     /gene="dapD"
                     /locus_tag="ACHOQV_000212"
     CDS             complement(38944..39768)
                     /gene="dapD"
                     /locus_tag="ACHOQV_000212"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFP9628644.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(39799..42471)
                     /gene="glnD"
                     /locus_tag="ACHOQV_000213"
     CDS             complement(39799..42471)
                     /gene="glnD"
                     /locus_tag="ACHOQV_000213"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFP9628645.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(42533..43327)
                     /gene="map"
                     /locus_tag="ACHOQV_000214"
     CDS             complement(42533..43327)
                     /gene="map"
                     /locus_tag="ACHOQV_000214"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP9628646.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            43695..44420
                     /gene="rpsB"
                     /locus_tag="ACHOQV_000215"
     CDS             43695..44420
                     /gene="rpsB"
                     /locus_tag="ACHOQV_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP9628647.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            44678..45529
                     /gene="tsf"
                     /locus_tag="ACHOQV_000216"
     CDS             44678..45529
                     /gene="tsf"
                     /locus_tag="ACHOQV_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP9628648.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            45676..46401
                     /gene="pyrH"
                     /locus_tag="ACHOQV_000217"
     CDS             45676..46401
                     /gene="pyrH"
                     /locus_tag="ACHOQV_000217"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP9628649.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            46693..47250
                     /gene="frr"
                     /locus_tag="ACHOQV_000218"
     CDS             46693..47250
                     /gene="frr"
                     /locus_tag="ACHOQV_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP9628650.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            47342..48538
                     /gene="ispC"
                     /locus_tag="ACHOQV_000219"
     CDS             47342..48538
                     /gene="ispC"
                     /locus_tag="ACHOQV_000219"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFP9628651.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            48727..49485
                     /gene="ispU"
                     /locus_tag="ACHOQV_000220"
                     /gene_synonym="uppS"
     CDS             48727..49485
                     /gene="ispU"
                     /locus_tag="ACHOQV_000220"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFP9628652.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            49498..50355
                     /gene="cdsA"
                     /locus_tag="ACHOQV_000221"
     CDS             49498..50355
                     /gene="cdsA"
                     /locus_tag="ACHOQV_000221"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9628653.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            50367..51719
                     /gene="rseP"
                     /locus_tag="ACHOQV_000222"
     CDS             50367..51719
                     /gene="rseP"
                     /locus_tag="ACHOQV_000222"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFP9628654.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVTSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            51749..54181
                     /gene="bamA"
                     /locus_tag="ACHOQV_000223"
     CDS             51749..54181
                     /gene="bamA"
                     /locus_tag="ACHOQV_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFP9628655.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            54303..54788
                     /gene="skp"
                     /locus_tag="ACHOQV_000224"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             54303..54788
                     /gene="skp"
                     /locus_tag="ACHOQV_000224"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFP9628656.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            54792..55817
                     /gene="lpxD"
                     /locus_tag="ACHOQV_000225"
     CDS             54792..55817
                     /gene="lpxD"
                     /locus_tag="ACHOQV_000225"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP9628657.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            55922..56377
                     /gene="fabZ"
                     /locus_tag="ACHOQV_000226"
     CDS             55922..56377
                     /gene="fabZ"
                     /locus_tag="ACHOQV_000226"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9628658.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            56381..57169
                     /gene="lpxA"
                     /locus_tag="ACHOQV_000227"
     CDS             56381..57169
                     /gene="lpxA"
                     /locus_tag="ACHOQV_000227"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFP9628659.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            57169..58317
                     /gene="lpxB"
                     /locus_tag="ACHOQV_000228"
     CDS             57169..58317
                     /gene="lpxB"
                     /locus_tag="ACHOQV_000228"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFP9628660.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTTRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHMLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            58314..58910
                     /gene="rnhB"
                     /locus_tag="ACHOQV_000229"
     CDS             58314..58910
                     /gene="rnhB"
                     /locus_tag="ACHOQV_000229"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP9628661.1"
                     /translation="MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRVLGLAS"
     gene            58947..62429
                     /gene="dnaE"
                     /locus_tag="ACHOQV_000230"
     CDS             58947..62429
                     /gene="dnaE"
                     /locus_tag="ACHOQV_000230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP9628662.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            62442..63401
                     /gene="accA"
                     /locus_tag="ACHOQV_000231"
     CDS             62442..63401
                     /gene="accA"
                     /locus_tag="ACHOQV_000231"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP9628663.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            63500..65641
                     /gene="ldcC"
                     /locus_tag="ACHOQV_000232"
     CDS             63500..65641
                     /gene="ldcC"
                     /locus_tag="ACHOQV_000232"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFP9628664.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGERWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            65698..66087
                     /gene="yaeR"
                     /locus_tag="ACHOQV_000233"
     CDS             65698..66087
                     /gene="yaeR"
                     /locus_tag="ACHOQV_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9628665.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            66152..67450
                     /gene="tilS"
                     /locus_tag="ACHOQV_000234"
     CDS             66152..67450
                     /gene="tilS"
                     /locus_tag="ACHOQV_000234"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP9628666.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRMENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELVQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDIAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIIGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(67499..67759)
                     /gene="rof"
                     /locus_tag="ACHOQV_000235"
     CDS             complement(67499..67759)
                     /gene="rof"
                     /locus_tag="ACHOQV_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFP9628667.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGTTRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(67746..67946)
                     /gene="yaeP"
                     /locus_tag="ACHOQV_000236"
     CDS             complement(67746..67946)
                     /gene="yaeP"
                     /locus_tag="ACHOQV_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFP9628668.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            68112..68657
                     /gene="yaeQ"
                     /locus_tag="ACHOQV_000237"
     CDS             68112..68657
                     /gene="yaeQ"
                     /locus_tag="ACHOQV_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFP9628669.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFSNLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            68654..69076
                     /gene="arfB"
                     /locus_tag="ACHOQV_000238"
     CDS             68654..69076
                     /gene="arfB"
                     /locus_tag="ACHOQV_000238"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFP9628670.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            69090..69800
                     /gene="nlpE"
                     /locus_tag="ACHOQV_000239"
                     /gene_synonym="cutF"
     CDS             69090..69800
                     /gene="nlpE"
                     /locus_tag="ACHOQV_000239"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414734.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFP9628671.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEGNPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(69830..70654)
                     /gene="yaeF"
                     /locus_tag="ACHOQV_000240"
     CDS             complement(69830..70654)
                     /gene="yaeF"
                     /locus_tag="ACHOQV_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFP9628672.1"
                     /translation="MDKPKAHCRLLLPCFLFLSACTVDISQPDSSATAVDVEAKTWVV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQVCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(70707..72425)
                     /gene="proS"
                     /locus_tag="ACHOQV_000241"
     CDS             complement(70707..72425)
                     /gene="proS"
                     /locus_tag="ACHOQV_000241"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP9628673.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(72536..73243)
                     /gene="tsaA"
                     /locus_tag="ACHOQV_000242"
     CDS             complement(72536..73243)
                     /gene="tsaA"
                     /locus_tag="ACHOQV_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFP9628674.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPNASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(73240..73644)
                     /gene="rcsF"
                     /locus_tag="ACHOQV_000243"
     CDS             complement(73240..73644)
                     /gene="rcsF"
                     /locus_tag="ACHOQV_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFP9628675.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAESAKPKVP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(73762..74577)
                     /gene="metQ"
                     /locus_tag="ACHOQV_000244"
     CDS             complement(73762..74577)
                     /gene="metQ"
                     /locus_tag="ACHOQV_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFP9628676.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(74617..75270)
                     /gene="metI"
                     /locus_tag="ACHOQV_000245"
     CDS             complement(74617..75270)
                     /gene="metI"
                     /locus_tag="ACHOQV_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFP9628677.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(75263..76294)
                     /gene="metN"
                     /locus_tag="ACHOQV_000246"
     CDS             complement(75263..76294)
                     /gene="metN"
                     /locus_tag="ACHOQV_000246"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFP9628678.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAITWLQEHH
                     VKVEVLGYV"
     gene            76482..77054
                     /gene="gmhB"
                     /locus_tag="ACHOQV_000247"
     CDS             76482..77054
                     /gene="gmhB"
                     /locus_tag="ACHOQV_000247"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFP9628679.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        18633 a        18842 c        20734 g        19064 t
ORIGIN      
        1 tgattatcag gcaacctgta ccggaatcgc tttcgcggta cgtttcattt cattgtcgcc
       61 ttcaaaatag gcgacgttgg gtcgccaggt gcgagcttct tcatctggca tagtaacgaa
      121 gctggcgatg atgacaatat cgccgacgct ggcgcagtgg gccgccgcac cgttaacgga
      181 aataattctc gaaccgcgtt ctgccgcgat ggcataggtg gagaaacgct tgccgttggt
      241 cacattccag atatcaatgg cttcgttttc gagaatacca gctgcgtcaa gaaaatcctg
      301 gtcaatggcg caagaacctt catagtgcag gtccgcatga gtcactttca cgcggtggag
      361 tttgccctgc agcatcgtgc gaatcataac ttctaccttt ctaccctgtc gttaacgaag
      421 caggcgatgc ctgctttgag gaaattctca cgcagtattg cccgattttt gtttagtgtc
      481 tactcatctg acggcatttg catcagcagt ttgcgtaccg tgccgaagcg tgccatgtaa
      541 cgcctgtaac caattgaaat ttctctgaac gctgctcggt aaacaataaa gaggcggctg
      601 acgacggcgc gccttgactg gatactgcct tcacgcaaga caacacggaa ggcagggaag
      661 atggatgcac cgagtaccac accgcatgac gcggtattta aacaattttt aatgcatgcg
      721 gaaacggctc gtgactttct ggatatccat ttgccagcgg aactacgcga actgtgtgac
      781 ctcgacacgc tgcatcttga gtcggggagt tttattgaag aaagcctgaa agggcacagc
      841 actgacgtgc tttattccgt gcaaatgcag ggtaatcccg gttatttgca tgtggtgatt
      901 gaacatcaaa gcaagccgga taagaaaatg acctttcgca tgatgcgcta tgccattgcc
      961 gccatgcacc ggcatctgga ggcggatcac gataagctgc cgctggtggt gccgattctg
     1021 ttttatcagg gcgaggccac accttatccg ctctcaatgt gctggtttga tatgttttac
     1081 tcgccggagc tggcgcgacg cgtttacaac aaccctttcc cgctggtgga tatcaccatc
     1141 acaccggatg acgaaatcat gcaacatcgg cggattgcga ttctcgaact actgcaaaaa
     1201 catattcgcc agcgcgactt aatgttattg ctggagcaac tggtcacgct gattgacgaa
     1261 gggtacacta gcggaagtca gttagttgcc atgcaaaact atatgctgca acgcggtcat
     1321 actgaacaag cggatttgtt ttatggtgtg ctgagagaca gggaaacggg aggggagtct
     1381 atgatgacgc tggcgcagtg gtttgaagag aagggaagac aggaggaaag gcaggaggta
     1441 aggcaggagg taagacagga ggtaagacag gaggtaagac aggaggtaat acaagaggtt
     1501 agacaggaag taagacagga atacgccctg cgttttctga gtaaagggat gtctcgggaa
     1561 gacgttgcag agatggcaaa tttacctctt gctgaggttg ataagctgat taactaagtt
     1621 atctgcatta tcagttatag ccagataatt gttattgtta actgaccaga taattaaggg
     1681 aacatatgga aataaagaca cggagagaaa ccaggcagac gttggcgcag tggtttgaag
     1741 agaagggatt tcagaaggga tttcagaagg gatataaaga gggactccag aaggtaagac
     1801 tggcccagcg tcttctgagt aaaggaatgt ctcgggaaga cgttgcagag gtgacagctt
     1861 tatctcttgc agagattgat aagctaatta actctaacta agttgtctac gatatgaata
     1921 attgcctaca gataacgtgg caatgaataa ctggcgccat cagccgtagc gccagataag
     1981 gattacgcca gttcgacgat tttgttgtcg atcaggcggg catcgccaag ccaggcggct
     2041 accagaatca ctgcacgttt gctgttttca gagacctcca gcaaggtgtc ggcatcacga
     2101 atctgaatat catcggcgcg gaagcctttt tcattcagtt cttgcccggc aatagtaata
     2161 atttcatcga gatcccgttc gccagcctgc aatttgtcgg caatcgaact taaaactttg
     2221 tacagaccgg gcgcaatttt gcgttgttcc gccgtcagat aaccgttacg ggaactcagc
     2281 gccagaccgt ctttggcgcg cataattggc acaccgacaa tctcaatatc aaagcccata
     2341 tcagcaacca ttttgcggat cagcgccagt tgctgaaaat ctttttcacc gaagcaggcg
     2401 atgtccggct ggaccaggtt gaacagcttg ctgacaatcg tcgaaacgcc gcgaaaatgt
     2461 cccggacggc tggcaccttc cagcatggtg gaaagaccag gaacatcaac ataggtgtgg
     2521 gtttctgtac cgttcgggta gatctctttt accgaagggg cgaaaactaa atccacttta
     2581 cgtttgttta gcttttcgca gtcctcttgc aaggtccgtg gataacgtgc cagatcttcc
     2641 gggcggtcaa actgcatcgg gttaacgaaa atactgacca ctaccacatc ggcgcgggct
     2701 ttggcttcgt cgaccagctt catatggcca tcgtgcaggt tacccatggt gggcaccagc
     2761 gccacgcgct taccttccat acgcaggcgg cgaatttgct gacgcagcag cggcagggtt
     2821 tcgataatta acacaacgtc actccttaat ggaaactgtg ttcttcgccc gggtaaacgc
     2881 cggactccac ttcagccata tactgccgca cagccgcgcg gatgtcgccc gtttcggcga
     2941 ggaaattttt agcgaattta ggaatgtggc cgccggtaat gccgaacgcg tcgtgcatca
     3001 cgaggatctg cccgtcagtg acgttgcctg cgccaatgcc aataaccggg atcgccagtg
     3061 cttcggtaat acgttttgcc agttcaaccg gcacgcattc cagcaccagc agctgtgccc
     3121 cagcagcttc taatgccaat gcatcgctga gcagttgatc gcccgcttca tcgccgcgcc
     3181 cctggacttt gtagccaccg aaaatattca ctgactgtgg ggttaggcct aaatgaccac
     3241 aaactggaac ggcacgttcg gtcagcattt gtacggtttc caccagccac tcaccgcctt
     3301 caattttgac catgttggct ccggcacgca ttaccgttgc ggcgttttca aaggcttgtt
     3361 ccggcgtggc atacgccata aatggcaggt cagccagcag aaggcagttt ggcgcgccgc
     3421 gacgcacagc ggcggtgtga taggcgatat cggcaacggt aaccggtagc gtggagtcat
     3481 gcccctgaac cgtcatgccc agtgaatcgc ctaccagcat gacgttaagt ccttcttcgg
     3541 caaacaattt tgcgaagcta tagtcgtaag cggtgatggt tgcgaaacgt tttttgtcct
     3601 gcttgcattt ctgcaatgaa gcaatcgtgg ttggtttcat agcgtatcct gataaatgat
     3661 gttgtgctgt ctcgcatttt atcagtcaca ttggtggggg caatgattta tccgtatcgc
     3721 accgccattg tctggcggtg caatagtgtt atcaggggta ggtaacctga aaagtgctgg
     3781 tggctttaaa atcccccggt tctatagcaa tattaccgtc ttgttttaat gtagcctgga
     3841 aatgaagagg ttgtgaggta ccgtttcctt tatccgggta aattcctccg gtagtgtcat
     3901 cttctgtttc gtaatcttta taaacagacg ttgaatcatt tggtttcagt accatttgtg
     3961 cacttttagt gttttttaaa ccttcaatca gtacaccaac accttttgct gcgttatttc
     4021 ctgtgagtgt gttagccaga agttctttgc ttacactgcc caccttgttt gacataagtt
     4081 ttgtttctat attgcgaaca cgaatacagt tctgtagcgt gatatcaaaa ggaaccgctg
     4141 ttgcgccatt ttttatttgc gctggggtat aatctcccat tttaactgtt gaaccgctta
     4201 cagatgggcc agtaagtgca gcagtaaaac acgttgggtt ggataacgtc atattatcca
     4261 ggtcaaaatc tattttaagg aaatagctgt gatcgttgat accgccacca agaccatatc
     4321 ctttgaatga gtagagataa ccacccggct tagataatgt taccttgttt gtctttgtgg
     4381 ggtcaaagtt ggtatcggta taaaattcca ccgtgagcga ctgccaaact ccacctacgg
     4441 cccagtattt attagatgag tttgcattgt tacaagacca atatagagtg gcttcacttt
     4501 cggggatcca ccgaaaatat tgagttggag aatcgccgat atacatacct gatgaactga
     4561 tattggttgt ggtgtatgat gaccaaatgt ttgatatctt taatgtataa tacaggcctg
     4621 ggacagatgt ttcaaatagt ttatgcccgc cataacgttt acccgaatca accatttgat
     4681 tttcaattgc cataggctca ccgcttgcag tgttacttct gttgcagtaa aggtaacctc
     4741 cacctgaagc gctatttaac tgagaccagt ataatgacac acctgttggt ccgagttggg
     4801 ttggtttata aaaaactaag ttatttgtgc ttgttgaata cctgaatttg acagtaatgt
     4861 ctgcactggg gcctgtttct ctccactttt ggtctcccgg acccactccg taatcattcc
     4921 caacaagata gctgacgtta tcatcggtgc cggcacttgc tgtataacaa caagagcata
     4981 gtgctaaaaa aagaaagtat ctgaagaata tcttcatttt tactccattg tcatgctgca
     5041 agataatgct tgcagcactt tgtctgttat tcgtagtaaa tatccactaa cacatggctt
     5101 ataagttggc cagaggtgac attcagcgct ggatcgattt tttgcattcg agtgctaaat
     5161 ttccaggatg aattgtttag tgctgatgac atgaccggat aaacggaacg tgagctacca
     5221 gttacgccaa gaacgttgaa ggtatttgcc gggtcctgaa tagagaatat aactacccca
     5281 acatttttag ccccggtgtt gtcttcattg gcgaatattt gactggaacc tgtcgccatt
     5341 acgccactga gtgcgcgaaa atcaatatgt aactgattta acacattccc tgtgccctga
     5401 agtgtgcagt cctgtactgt aatagtgaac tcttttccac cggggtgata attttcggct
     5461 gaaatgtttc cggtaaaatc gcccacgcca acgacaccta aatcaatatt tccgttgttg
     5521 ctgattccac tttggcaggt gttatttttt atatttgcaa caatgtcaac atcctgtcct
     5581 gcaaccacag ggaaagtggt gctgaatagc aaagacagaa gcagaattga ttttttcatc
     5641 agattacgtt gagtggggcg catatttagt tcctgaatcg gtctgcctgt tagttataag
     5701 tcacagtaaa tgttgccgga gcggtgaaat cacccgcggt gacatctgta acggttttat
     5761 ctttcgcgac caccaaaaca gcactcatcg ggtaatcgac aacatcacct cccgtcgcac
     5821 cagcaatact gacttcttct gttgcggggg tgacgcaact gaattgtttt gcgccgtttt
     5881 tacctggtgt ggacgtactc cagacctcaa cggcgactgc cgctgctttt gctgttgccg
     5941 ttgatgcgtt agcaaatcca gggccgttat tagaattacc ttcgcataag gctcgtgggg
     6001 tcaactttat tttggccttt ttcccgggaa tccccgcaca gtctttgaac tgcaatttga
     6061 aggttttaat tttagtttcg gcattaattt cagaaatata tacatcaccg aaattgatgg
     6121 tggacagttt agctgtaccg tcatcagtca acgttgatgt acaggtaccc atttctatgt
     6181 ctgtagtaac agtcaggcca atactatcag tggccgcatg tgaggaaatg gttaacagtg
     6241 atgaagccag aaatagacta cccgataatc cataacataa tttttttaat atcatcattg
     6301 tcatccttgt tattgataga tgaaattgaa cgttgcctgt gcgcgaaatt tcccgggtat
     6361 gccctgtcct ggggttgttt cgcgcagtgc gacagtcatt tccagaccac tggtgctaat
     6421 ttctgtggtt ttccagcgga tgtattccgc gctattgggt ttcagaaacg tatcgcccga
     6481 agtctctaat cttttaaatc ccataccaat attactggtt gttgaggagg agtcagaaga
     6541 ttgtggaata atcagcgatg tactaccaga ctgatttcca gtcattgtgg tatcaatcca
     6601 gctaattcca ctgctacacc cactggcgac taatttgaag ttggcctcag attgtgacgc
     6661 tttattgact atcttatcca gtcccatctt ggggattatc agggtataac tatcattgcc
     6721 attatcaacg acgtttgaac cattcagcga tgtcagggtg atgttacatg ttgtcgcttt
     6781 cacggtagcg gtaaaatcga cattaacatc cgttgcaaat acagatggcg ataataatag
     6841 ccccatgagt attgctattt tatgtggtgt ggtttttatc attggaagtc ccttgtggtt
     6901 actgtatctg acaacgaagt ccgttcagaa taatagattg cgctattttt tctgatgtcg
     6961 tgttttgttg gtaatgagca aaacaactga ctggtttgcc ctcttcaatc caggtaattc
     7021 gtatatttcc ttcctgcccg ataccgcgaa caaaggcctg accaccctga cctacgttgc
     7081 cgatgatatt gttttgttca tcataaatat ccgctgcaaa tggaatattt ttaccatcgc
     7141 ttcgcacgat attcataatt gccgattgtc cctgaactgt ttcaaaatca gcaaagacga
     7201 cggctccctg gcgcgggacg gcgacagtac tggtgctttt caattcaaca tcgttttcca
     7261 gatcgttaat gtccagtgcg atacggtttt catggtatgg cgaaagggcg ctggtcacac
     7321 cataacccca gcggtctacg gtgctgtttc cgtagttaat acgagcgcct tttgctcctg
     7381 gcgcctgaat gacagccaga gtgtcggaat cactaaaact gtcattactg aacgtcagcc
     7441 cgccgctatg taacacaaat ccaccatcag tattgagtga gaattggcga ctattatcac
     7501 tgcttgctga ggctgaacct gacagagttc cccacggcga ttcataactg gcataaccgc
     7561 cgatataact caggtcatca ctggatttat tcatcatata acccgtgttt acgctgtagc
     7621 tgatgcgatt ggtatcgctg taaccactac tgctgatatt gagttggtta ttacccttaa
     7681 agtcgctgtt taattgtgta tcaatactct ggaagccgga gctgcggtgt tccgtaccca
     7741 gtaaattttc tattggaatc gtgaagctga gataaatgct atcgtcggtt tgaccatctt
     7801 cattcaacga acgttgagca ctgatactgt aactgcccca ggacgcacta ttgctataac
     7861 caatagagta attagtactg ttttgcccgg aggcccagta atctgaccag cttcccgaga
     7921 gataaaatga accgtaatcc tttttctcaa atttcagcgg ttggttgata ctgaccgtga
     7981 cctgattttt catgcgcgag taattgcgca tggatttagg ctcaagatct tgctcaggat
     8041 gttctacttc atcaatcagc gtcagcgcat cattgaggcc cagataatgc tgggtggagt
     8101 agcgataggc cgcgatattt aatgatgtac tggtattttc aaataacttg ttccaggaaa
     8161 tacggtagct ttgtccttga taggttttat cgtccggaat gctgacattt gagtgagtaa
     8221 catcgacaga aaaagccccc acgggggtat tcataccaag ccccagcaat ccggcggtat
     8281 agttattatc agttagctga ataccggtat atcctgtcag gtagttattc aaaccatagt
     8341 agtagctggc ttgaaataaa ttaggctcat cctggatact gtcttttaaa acctgaccgg
     8401 cgctaatatc ccagcgacca acgccagggc gcagcatttg cacaacagat gaaaatggct
     8461 gggagaacgt ccgttttgtg ccatcagctt cttcgatagt cacaatgaga tcactgccat
     8521 aacctgacgg gctaagatca tcaatggcaa aggcccctgg cggcaccgtc gtttcataga
     8581 ttttataacc attttgcata acggttactt tggcattggt attcgctacg ccatgaatga
     8641 ttggcgcaaa actggctaat accggaggca gcattcgact gtcactgtat aagcgaatac
     8701 cgcgaatact gactgaatcg aaggtttcgc cggttgtgta agattcacca ataaccagtt
     8761 gtgaacgtag tgacgcaaga tcgcgttgta aatagcgatt ctgaaaatcg taatcgctat
     8821 ggacattggt catccagttg tagttacccg aggcacgtaa ccgccaggca cctaagttta
     8881 tcccaccgtt gaatgccgcg tagatactgt catttgttct gcctggactt tcactgtgat
     8941 atccgttgag gttgtaggaa agcatggcag cattaatgcc attttcccac aaagagggat
     9001 caacatagtt ttgataattc ttcataatcc atgcctgggg gacgtcgata tccaggcgtt
     9061 gatcgttaac atcgtaacga atggatgatt gagggatcat ttcagccaga ttaaggcaat
     9121 caccaagatc ttcgtctcgc ttaagcaaaa tggctttttc gctattcttg tctggtgaac
     9181 tgatatgaaa ttgcaataaa tttttctgcg taatgcaggc ctgagcgttc tttttacctt
     9241 caataacagt aaaagcaatg ctttggctca tgataggttg atcgttaaca tacacactga
     9301 catcgtaaat gcctggtagt gtcgggttgc cttctgtata ccgactaagg tcgatatttg
     9361 aagcatcctt tcccatcaga aatgtatggt catattcgac gagttcagcg cataaagcac
     9421 tgttgctata cagcagcgcg caaaacgtgg cgatgcgggt aaggtgatta tgatattttt
     9481 tagtggattt aatagtcacg ttctgcttcc tgctgtggta tatccttgcc agattaaagg
     9541 ttggcgttgc cttcaataga gccgccaaaa tcattaatgg cgaaaaaatg cacttttgca
     9601 gagcttgttc tgccactcat cccatttacc ttgatgacgt cgttactgaa cggtgcaatc
     9661 attttcacat ccaggggata gcgttttccg ttggcttcta aatcaccact gctaaaagag
     9721 acatagtagg gcgtagggtt atcaacgcgt aatgccgctt tgccttcaga tgttgcccag
     9781 gaccacttca gagccaatgg cgcatcagat ggaattccac tcaatccttc cgggcgatag
     9841 aacagtttta tacgtgtacg aaatgccagt tgtagcaggc tttgatttgc tgcttttgct
     9901 gcatcgggtt ttggtggaac ttccagtacg ttaaaccaga acacactttc tctgtctttt
     9961 ggcagagcac tactggcggt gtacatcagt ttgattgttt ggccacgttt agcatcaata
    10021 cgtgatactg gtggcgtagc ggtaaagggg acagtaatac tgcctggttc agcgttgtca
    10081 tctccagtat ctaaccaact ctgaaccagc aatgggttat tgcctttatt ttccagacgc
    10141 acattgacgc ttttttgttc gcttttataa actacgcgtg tacctgaaat tacgatgtct
    10201 gcaatagctg atgagctaaa agccatacag ctcgctaagc acaatgctgc ggtatgttta
    10261 gtattaaaaa acataaaccg ttcctcacag agttaattaa aacagggaat aacgttcaaa
    10321 aataaatatg tttgatgtta ttccctatat atattcaccc aatcaattta ggtggctgat
    10381 atttactggt aagtaatggt atatgctgag ttagttttta cataaccggc agttgctgct
    10441 ttgcttgcat cagcacgaac ataggatgct ttaaaatcgt aggttgcagt tttagacgtt
    10501 gcatccagag ttttgctgta aacatcaccc gggttattaa cctgaacctg cttaatagtg
    10561 gaaccttcaa tattatgcag ggcaatattc acaccatcag atggattatt gatagacata
    10621 tcgttattca gagtaccttt gctattgcta aagaacacgg aaccgaaagt catttttgca
    10681 gtagtacccg gagccgggtt agctttgctg cagtcaatag taatgctgaa tggcttagct
    10741 ttagcaccta cggtggtatc cagtacacca tcggtgattt cagcaacagt agcggtttgc
    10801 agcaggatca aaccatcttt gttaccgcca tcaacacgag cttcacaggt gttatcaacg
    10861 accaggccgc taatatttaa ctgaccaccg tccatatcgt tagcagcaaa tgcagtacca
    10921 gcagccattg ccaacataat accagacagc gcaaaaccta attttttaga cataaaaatc
    10981 cttgaataat attccataga gagataaccg caccgcatga cgataagagt ctgctgcaat
    11041 gctgcgtaac aaaatcgtta ctgtgttaat gatgtttgtc tgttgtttac caactattac
    11101 attcattcat catcgggatg gctattttgc ataatgtgat agcatatgca atttaatttt
    11161 actaaaaata aaagttagta aaattaaatt tgaaagctaa gagggatggt gtatggcgtg
    11221 agaattacgg gaaggtatat tatattttct aaaattttat ttatcttaat gatattgttt
    11281 tggcaataac ttgaggttgt tttcagaata tatttatatt cacacgaata ataattacca
    11341 cttatctaat tcattaaagc cttttgcctg tagagcttca actaatgata agccatcagg
    11401 aaatagcaat tccggcgcaa tttcaaacag tggccacagc ataaagccgc gatttttcat
    11461 atcgtagtgc ggaacggtca gacgttcggt atttattact tcattaccaa acagcatgat
    11521 gtcgagatcc agcgtgcgcg gtccccagcg ttcagcttta cggacgcgac cttgctgcaa
    11581 ttcgatacgc tgtgtgtgat tgagtagctc ttcaggggca agagtggttt tcagcgcgac
    11641 ggctgcattt aagtaatcgg gttgatcttg cggccccagc ggtggggtgc ggtaaaacga
    11701 agaaacagca agaatgcggc tttctggaat atcgcctaat gctttcaggg cagcattgac
    11761 ctgctccagc ggagaggcca gattgctgcc tatggcaata tacgccactg tcatgcggta
    11821 ccctcacgac gcggtgcgcg tttgcgtgga cgacgagtac ggcgacgcgg tgacggttct
    11881 tcatccagtt cgttaagcat ccctttttgg tctggtggcg cggaaacctg gaactcaccc
    11941 caccatttca ccagacgttg cagttcagcg ttacgctcaa cttcggctcg taaggccaac
    12001 aggtcgtatg ccgcacggaa cttaggatgc tccagcagtt tccaggcgcg tttaccctga
    12061 cgtcgggaca tacgcaactg caactgccag atatcgcggg ttaacgtcgt cagacgtttc
    12121 gggatcgcca gtgaacgaca ggcttcgtcc agcacgtcgt tcatcgccag cgcgaaagcg
    12181 tcgtgatagg tcaggccgct ttcctgggcg atcttctgtg ccgtctccag cagtgggtac
    12241 cagaacatgg cggcaaacag gaacgccggg ttcacgcgca tatcgttatg gatacgcgta
    12301 tcggtattct tcagcacctg ttcaatgatc cgctccatcg ggctgtcgcc attttccgtg
    12361 aagtagcggg taatggtcgg gaacagcggc tggaacaggt gatattcaca cagcagctta
    12421 taggtttcgt aaccgtagcc cgcttgcagc agtttcaaag attcttcaaa cagacgtgcc
    12481 ggtggaatat cgttcagcag ggtagccagg cgagggatcg gttctgcggt ttcaggactg
    12541 atacgcatac ccaatttggc ggcaaaacgt accgcgcgca gcatccgtac cggatcttca
    12601 cggtagcgcg tttccgggtt accaatcaga cggataacgc cgtccttcag atccttcatg
    12661 ccgccaacgt aatcacggac ggtaaaatct gccacgctgt aatacaggct gttgatagtg
    12721 aaatcgcggc gctgggcgtc ttcttcgatg gagccgaaaa tgttgtcgcg cagcaacatg
    12781 ccgttttgct cgcgttggga ggtcgtgcgg tcgctgacgt taccttcgtg atgtccacgg
    12841 aaggtcgcaa cttcgataat ctccgggcca aacatcacat gagccagacg gaaacggcga
    12901 cccaccaggc ggcagttacg gaacagttta cgtacctgtt caggcgtggc gttagtggtt
    12961 acgtcaaaat ctttcggctt tttgccaagt aacaggtcgc gcacgccacc gccaaccagc
    13021 caggcttcgt atcccgcttt attgagcctg tacattacct tcagggcatt ttcactgata
    13081 tctttgcggg aaatagcatg ctgctcacgc gggatcaccg tcacctgtgg acgggcgact
    13141 gcctgttcag cctcgctttc ctcgcggctt agcaccttgc ggcaaaaatt agcgactcgg
    13201 gtaaaaatag tacacctcgg tagtgtcaaa catcattcag gacaaaaaaa tagcggctaa
    13261 tcatagctca gcatgacgca tttgagaatg ttgaatttac aattgccgac tcaggcacgg
    13321 cggtaagccg ccagtttttg acggctgact gaaggatttg ctcgacgctg aaatcctgcc
    13381 agtgtgcttc tgcctgctga cccagaaatt gaagtgccgc gattagtacc gggcgcggat
    13441 cgcctttcgg caacgcaggc gcatgattct gcttggaaag tttagcgcct tgtgtgttaa
    13501 gcgccagcgg taaatgaatg taatctggca ctttccagcc aaaaagctgg tacagcgaga
    13561 tttgccgcac tgtcggttcg atcaaatctg ccccgcggac aatttcgctc acgccctgga
    13621 agtgatcatc caccaccacc gccaggttat aggcaaacaa accatcacgg cgatgaataa
    13681 taaaatcttc ccgcgccagt ttttcgttgg cgtgaataat gccgcgcagc tgatcagtaa
    13741 atagcgtgac cggatgctgc tggcggatac gtactgcggc gttgtctggt ccatgatgca
    13801 attcccggca atgaccgtcg taaataccgc caatgctttg aatacgcgcg cgcgtgcagg
    13861 tgcagtaata acttagtcct tgctcatgta accaggcgag tgcttcgcga taggcatcgt
    13921 gacgttgcga ttgccagaga acgtcgccgt cccagtgcag accgtaatgt tccagctggc
    13981 gcaggatagt ctctgcggca ccgggaactt cacgaggcgg gtcgatatct tcaatgcgta
    14041 ccagccagcg accttgccgg gcgcgagcct gtaaatagct gccaagcgcg gcaatcagag
    14101 agccaaaatg aagctcgccg gaaggagagg gggcaaagcg gccaatatag tgtgtgtctg
    14161 tcatctcgtt gaacaaaaaa taaggcggga gcatttcccg cctgtggtaa acgtgatgga
    14221 acggctgtat ttagccagcc atttgttttt cgcgaatttc agccagcgtt ttgcagtcga
    14281 tgcacagatc ggctgtcggg cgcgcttcca gacggcgaat accaatttca acaccgcagg
    14341 attcgcagta gccgaaatct tcgtcttcca cttttttcag cgtcttctcg atctttttga
    14401 tcagcttacg ctcgcgatcg cggttacgca gttcgaggct gaactcttct tcctgggctg
    14461 cacggtctac cgggtccggg aagttggctg cttcatcctg catatgtgta acggtgcgat
    14521 cgacttcatc cctgagttga ttacgccatg cttccagaat acgacggaag tgcgccagct
    14581 gggcttcatt catatactct tcgcccggct tctcctgata tggttccacc ccagcgatgg
    14641 cgagaatact cagggacgat gttttacggt tttgcccttc ttgcatgttg cttctcctta
    14701 acacgcacta tcgatcccca tgttcggggg aaaaatgagg ccgctataaa tagcagatgc
    14761 ttttccggat agcaattatc taaacgtaac acttgacaac tgtgtgagga aaagcgtatt
    14821 tgcgcacgcg accagaatgt aaattaacca gttacatact ttactacaat gtaaccggca
    14881 gtgatttttt aagagccatg ccttcagcag aaagttccgc tttgtaagcc agaatttcta
    14941 cccccctctg ttgagcttct gacaatagtt gcgcgtattt ctcatcgatg tggcgcgcgg
    15001 gtgaaaaccg tgtaatggct gaatgcagca cggcgaaaaa gataaccgca cgctggcctt
    15061 cagccgctac gctcatcaac tcccgaaggt gtttctgacc tcgttcagtg accgcatcgg
    15121 gaaaatatcc ctgttcgttc tccgctaacg taaccgattt cacttcaata tagcagtctg
    15181 gacgcgaatc cgcctgcaac ataaagtcaa tacggctgcg ttcggcgccg tattttactt
    15241 cgcttttcag cgagctatag cctgacagct ctgaaattga ttcattaagg atagcctctt
    15301 tcgtcaacct gttagcccaa agcgtgttga cgcaaataaa tgcgccgctc tggctttgag
    15361 tcaattccca ggtgtgtggg tatttccgtt tggtgttgtc tgaagtcgaa taccagacgg
    15421 tatcgccagg cgttgcacaa ctggtcatcg cacccgtatt cgggcagtgt agcgttaatt
    15481 cgcgaccatc gggtgtgatc acatcggcta aaaaacgttt gtaacgctga attagcgtcg
    15541 cgcgctgtag agggggagaa aattccatta cgacaatcct tgttattgcg ttagcgccca
    15601 gcgttttagc ggcgtgtagc gtgtgcgtcc acgggcaaac gaggaggcgt aaagggtgaa
    15661 ctccgtcacc gtatacgacc agttaaaacc tggcggcggg attgtcaccg cctcgctggc
    15721 gtcgcgcaat aaggtaatat gtggatgaaa cggacgattg ctttgaaaac aaccgctgcg
    15781 ggcagcctgt gaacggagca tattcgccag ctggattaag ccgcgtggtg gctgacgcat
    15841 ccctaaccac accacacgcg aacgcagcca ttgaccggcg tcatcaagcg tgagtgtgaa
    15901 acccggttga cgaatccgcc cggctaaaag agaaagcgcc ttctctttct cggcgctcac
    15961 ttcgcctaaa aatgccagag tcagatgcaa attatcggcg gcgaccggac gtcccgcgtc
    16021 aggtgggaag tgtgtggcgc gccagtggat aatctgttcg cggatttctg caggtaagtc
    16081 gatagcaaag aacagccgtt gcggttcaga catgtgaggc actcggttat gaattaccgc
    16141 gatgctacaa tgtggcgcga agaatgttaa ccctctggag cgttttgtgt cgtcgttgcc
    16201 cgttgctgcc gtcttacctg agttacttgc tgctctcgat tgtgcatcgc aggtgttatt
    16261 aagtgcgccg accggggccg ggaaatcaac ctggctgccg ctgcaactgc tggcgcatcc
    16321 cggcattaac gggaaaatta tcctgctgga gccgcgtcgt ctggcggcgc gtaacgttgc
    16381 gcaacgactg gcggagctgc ttaacgaaaa gccaggcgat accgttggct accggatgcg
    16441 tgcgcaaaac tgcgtcgggc cgaatacccg cctggaagtg gttaccgaag gcgtgctgac
    16501 gcggatgatc cagcgtgacc cggaactgag cggtgttgga ttggtgatcc ttgatgaatt
    16561 tcatgagcgc agcttgcagg cggatttggc gttggcgctg ttactcgatg tgcaacaagg
    16621 tctgcgtgat gaccttaaac tgctgattat gtcggctacg ctggacaacg accgcttgca
    16681 gcaaatgctg ccagaagcgc ccgtcgtcat ctcagaaggg cgctcgtttc cggttgaacg
    16741 ccgttatttg ccgctgcccg ctcatcagcg ttttgacgaa gccgtcgcgg ttgccaccgc
    16801 cgaaatgctg cgtcaggaaa gtggatcatt actgttattt ttacctggcg tcggagaaat
    16861 tcagcgtgtg caggaacaac tggcttcacg catcggcagt gatgtgttgc tctgcccgct
    16921 gtatggcgcg ttgtcgctga acgatcagcg aaaagcgatc ctcccggcac cgcaagggat
    16981 gcgtaaagtg gtgctggcga ccaatattgc tgaaaccagt ttaaccatcg aaggtattcg
    17041 tctggtggtg gattgtgccc aggagcgtgt ggcgcatttt gatccgcgta cagggcttac
    17101 gcggctggtt actcaacgcg ttagtcaggc gtcgatgacg caacgtgccg ggcgtgccgg
    17161 gcgtctggag ccgggtatct gcctgcattt aatcgccaaa gaacaagcag aacgcgctgc
    17221 cgcgcaaagt gaaccggaga tcttacaaag cgatctttcc ggtttgctga tggaattact
    17281 gcaatgggga tgcagcgata cggcgcagat gagctggctg gatcaaccgc caacggtgaa
    17341 tctacaggcc gcgaaacggc tgttacaaat gctgggggcg ctggagggtg aacggcttag
    17401 cgcacaaggg caaaaaatgg cagtgctggg tagcgatccg cgtttagcgg caatgctggt
    17461 tagcgcgaag aacgacgacg aagctgctac cgcggcaaaa attgccgcca ttctcgaaga
    17521 gccgccacgg atgggcaata gtgacctggg cgtggcgttt tcgcgcaatc aacccgcctg
    17581 gcagcaacgt agtcagcaac tgttaaaacg cttaaacgta cgcggcggtg aggcagacag
    17641 ttcgcttatc gcgccgctac ttgccggggc gtatgccgat cgcattgctt gtcgccgtgg
    17701 gcaagatgga cgctatcaac tggcaaacgg catgggggcg atgctcgatg ccgacgatgc
    17761 gctaagccgc tacgaatggt tgatcgcacc gttattattg cagggcagcg cctcgccgga
    17821 tgcgcggatt ttactggcgc taccggtcga tatcgatgaa ttagtacaac gctgtccgca
    17881 gctggtacag cagtcggaca ccgtggaatg ggatgacgcg caaggcacgc tgaaagcctg
    17941 gcgtcggctg caaatcggtc agttgacggt gaaagtgcag ccactggcga aaccctcgga
    18001 agacgagttg catcaggcga tgcttaacgg catccgtgat aaaggtttaa gcgtgctcaa
    18061 ctggacggcg gaagcggaac agctacgctt gcgtttgtta ttcgccgcaa agtggttgcc
    18121 ggaatatgac tggccagcgg ttgatgatga gagtttgttg gcgacgctgg aaacgtggct
    18181 actgccgcat atgagcggcg tgcattcact acgcggcctg aaatcactcg acatttatca
    18241 ggcgctacgc ggattacttg attggggaat gcagcaacgt ctggatagtg aattgcctgc
    18301 gcattacact gtgccgacgg gaagccggat cgccattcgt tatcatgaag ataaaccgcc
    18361 cgcgctggcg gtgagaatgc aggagatgtt tggtgaggcc accaatccga cgatcgccca
    18421 ggggcgcgtg ccgctggtgc tggagttgct ttcccctgcc caaaggccat tacaaatcac
    18481 acgagatttg agcgccttct ggaaaggagc gtaccgtgag gtgcaaaaag agatgaaagg
    18541 gcgttatccc aaacatgtct ggccggacga cccggcaaat actgcaccga cgcgacggac
    18601 gaaaaagtat tcgtgaagac caaagcaaat ttgagagata tcttcttctg tcttgcaaca
    18661 gaagaacaga aaatcgggct tttgcgcctg aatattgcgg agaaaaagca tggccgggaa
    18721 tgaccgcgag ccaattggac gcaaagggaa accgacgcgt ccggtcaaac aaaaggtaag
    18781 ccgtcgtcgt tacgaagatg acgattacga cgattatgat gactatgagg atgaagaacc
    18841 gatgccgcgc aaaggtaagg gcaaaggcaa agggcgtaag cctcgtggca aacgcggctg
    18901 gctatggctg ctgctaaaac tggctatcgt ttttgccgtg ctgatcgcca tttacggcgt
    18961 ttatctcgat caaaaaattc gcagccgtat tgatggcaag gtctggcaat tgcccgcggc
    19021 agtttatggc cgaatggtca accttgagcc agacatgacc atcagcaaga acgaaatggt
    19081 gaagctgctg gaggcgaccc agtatcgtca ggtgtcgaaa atgacccgtc ctggcgaatt
    19141 taccgtgcag gccaacagca ttgagatgat ccgccgtccg tttgatttcc cggacagtaa
    19201 agaagggcag gtgcgtgcgc gtctgacctt tgatggcgat catctggcga cgatcgtcaa
    19261 tatggagaac aaccgtcagt ttggtttctt ccgtcttgat ccgcgcctga tcaccatgat
    19321 ctcctcgcca aacggtgagc agcgtttgtt tgtgccgcgc agtggtttcc cggatttgct
    19381 ggtggatact ttgctggcga cagaagaccg ccatttctac gagcatgatg ggatcagtct
    19441 ctactccatc ggacgtgcgg tgctggcaaa cctgaccgcc ggacgcacgg tgcagggtgc
    19501 gagtacgctg acgcaacagc tggtgaaaaa cctgttcctc tccagcgagc gctcttactg
    19561 gcgtaaagcg aacgaagctt acatggcgct gatcatggac gcgcgttaca gcaaagaccg
    19621 tattcttgag ctgtatatga acgaggtgta tctcggtcag agcggcgaca acgaaatccg
    19681 cggcttcccg ctggcgagct tgtattactt tggtcgccca gtagaagaat tgagcctcga
    19741 ccagcaggcg ctgttggtgg gtatggtgaa aggggcgtcc atctacaatc cgtggcgtaa
    19801 cccgaaactg gcgttggagc gacgtaatct ggtactgcgt ctgctgcaac agcaacagat
    19861 tattgatcaa gaactctatg acatgttgag tgcacgtccg ctgggggttc agccgcgcgg
    19921 tggggtgatc tctccacagc cagcctttat gcaactggtg cgtcaggagc tgcaggcaaa
    19981 actgggcgat aaggtaaaag atctctccgg cgtgaagatc ttcactacct ttgactcggt
    20041 ggcccaggac gcggcagaaa aagccgccgt ggaaggcatt ccggcactga agaaacagcg
    20101 taagttgagc gatcttgaaa ccgcgattgt ggtcgtcgac cgctttagtg gtgaagttcg
    20161 tgcgatggtt ggaggttctg agccgcagtt tgcgggctac aaccgtgcga tgcaggcgcg
    20221 tcgttcgatt ggttcccttg caaaaccagc gacttatctg acggccttaa gccagccgaa
    20281 aatctatcgc ctgaacacgt ggattgcgga tgcgccaatc gcgctacgtc agccgaatgg
    20341 ccaggtctgg tcaccgcaga acgatgaccg tcgttatagc gaaagcggca gagtgatgct
    20401 ggtagatgcg ttgacccgtt cgatgaacgt gccgacggtg aatctgggca tggcgttggg
    20461 gctgcctgcg gttacggaga cctggattaa actgggtgtg ccgaaagatc aactgaatcc
    20521 agttccagca atgttgttgg gggcgttgaa cttaacgcca atcgaagtgg cgcaggcgtt
    20581 ccagaccatc gccagcggtg gtaaccgtgc tccgctttct gcactgcgtt cggtaatcgc
    20641 ggaagatggc aaggtgctgt atcagagctt cccgcaggcg gaacgcgctg ttccggcgca
    20701 ggcggcgtat ctgacactat ggaccatgca gcaggtggta caacgtggta cggggcgtca
    20761 gcttggggcg aaatacccga acctgcatct ggcagggaaa acagggacta ccaacaataa
    20821 cgtagatacc tggtttgcgg gcattgacgg cagtacggtg accatcacct gggtaggccg
    20881 tgataacaac caaccgacca aattgtatgg agccagcggg gcaatgtcga tttatcagcg
    20941 ttatctggct aaccagacgc caacaccgct gaatcttgtt ccgccagaag atattgcaga
    21001 tatgggcgtg gactatgacg gcaactttgt ttgcagcggt ggcatgcgtg tcttgccggt
    21061 ctggaccagc gatccgcaat cgctgtgcca gcagagtgag atgcagcagc agccgtcagg
    21121 caatccgttt gatcagtctt ctcagccgca gcaacagccg caacagcaac ctgctcagca
    21181 agagcagaaa gacagcgacg gtgtagccgg ttggatcaag gatatgtttg gtagtaatta
    21241 acgtctaagc gtgacatacc ggatggcgag tagccatccg gtaaaataac atcccaccca
    21301 aagtattaac cctttctttt catctggttg tttattaacc cttcaggaaa gctcagattg
    21361 cgtaccgctt gcgaacccgc cagcgtttcg aatattatct tatctttata ataatcattc
    21421 tcgtttacgt tatcattcac tttatatcag agatatacca atggcgcgtt ccaaaactgc
    21481 tcagccaaaa cactcactgc gtaaaatcgc agttgtagta gccacagcgg ttagcggcat
    21541 gtctgtttat gcacaggcag cggttgaacc gaaagaagac actatcaccg ttaccgctgc
    21601 acctgcgccg caagaaagcg catggggacc ggctgcaact attgcggcga agcactctgc
    21661 tactgcgact aaaacggata caccaattga aaaaacgccg cagtctattt cggttgtgac
    21721 aaatgaagag atgcagatgc atcaatttca gtctgtaaaa gaagcattag gttatacacc
    21781 tggcgttacg gttagcagtc gtggtgcttc taatacatat gattttgtta ttattcgtgg
    21841 tttctcatct gttggtctga atcaaaataa ttaccttgat ggactaaaac ttcagggcaa
    21901 cttttataac gatgctgtga ttgatcctta catgctcgag agggttgaac tgatgcgcgg
    21961 gccgacgtct gttctctatg ggaaaagtaa tcctggtggg attatttcta tggtgagtaa
    22021 gcgtccgaca actgaacccc tgaaagaaat tcagtttaaa atggggacgg ataatctgtt
    22081 tcagaccgga ttcgatttca gtgatgcgct ggatgataac ggtgagttct cctaccgttt
    22141 gactggcctc gcacgctcaa caaacgaaca gcagaaaaac tctgaatctc agcgttatac
    22201 cattgctcca tcattctcat ggcgtccaga cgacaaaacc aattttactt tcctgtccta
    22261 ttttcagaac gaaccagaaa cgggttatta cggttggttg ccgaaagagg ggaccgttga
    22321 gccattgcct aatggtaagc gtctaccgac tgacttcaat gaaggtgcat cgaataatac
    22381 atactcccgt aaccagaaaa tggtgggata tagttttgaa catggtttta atgacacctt
    22441 caccgtgcgt cagaatctgc gtttcagtga aatgaaaacc tcacagaaaa gtgtttatgg
    22501 cacagggatt gccagcgatg gtcataccct aaaccgcggg acagtggtgg ataatgagcg
    22561 tctgcaaaac tttagcgttg atacccaact tgaaagtaaa tttgctacag gtgaagtcga
    22621 gcatactttg ctgacagggg tagacttcat gcgtatgcgc aatgatatca atgccagctt
    22681 tggatctgca ccatccatcg atctttataa caaatatcat cctgaatact ttgcatttgg
    22741 taacgcagag ccataccaaa tgaatgaaag caaacaaaca ggtatttatg ttcaggatca
    22801 ggcggaatgg aataaatggg tattcactct ggggggacgc tacgattggt ctaagcaagc
    22861 gaccactgtt cgtgaaaact cttatacgcc gactgaaggt tatattgagc gtaatgatca
    22921 tcagttcacc tggcgcggtg gtgtaaacta cttattcgat aatggtattt caccttactt
    22981 tagttatagc cagtcctttg aaccgagtgc tttcgatctg tggagcaacc cgcgcgtttc
    23041 ctataagcca tcgaaaggtg aacagtatga agctggcgta aaatatgttc cgaatgatat
    23101 gccggtcgtt gttacgggcg cagtctatca attgacgaaa acaaataacc tgacagcaga
    23161 cccaacaaac ccgttagcgc aagtcccagc aggtgagatt cgcgctcgtg gtgtggaact
    23221 tgaagcaaaa gcggcgttaa atgccaatat taacttgacg gcttcttata cctacacaga
    23281 tgcggaatac accaaagaca ccaatctcaa aggtaaaact ccagaacaag taccggagca
    23341 tatggcatct ctctgggggg attatacctt caatgaaggg ccgctttctg gtttaacatt
    23401 gggaacaggt ggtcgtttta ttggttccag ctatggtgat ccggcgaaca cttttaaagt
    23461 gggtagcgca gctgtaatgg atgctgttgt aaaatatgat ctggcacgct ttggtatggc
    23521 gggatccagc cttgctgtga acgtcaacaa tttgctcgat cgtgagtatg ttgccagttg
    23581 cttccagacc tatggctgct tctggggcgc agaacgtcag gtcgttgcaa ccgcaacctt
    23641 ccgtttctaa ttttttcttt ggggcacgga tttccgtgcc cgcttcacaa gttggctgtt
    23701 atgcaggaat acacgaatca ttccgatacc acttttgcac tgcgtaatat ctcctttcgt
    23761 gtgccagggc gcacgctttt gcatccgctg tcgttaacct ttcctgccgg gaaagtgact
    23821 ggtttgattg gtcacaacgg ttctggtaaa tccactctgc tcaaaatgct tggccgtcat
    23881 cagccaccgt cggaaggcga aattcttctt gatgcccagc cgctggaaag ctggagcagc
    23941 aaagcgttcg cccgcaaagt ggcttatttg ccgcagcagc ttcctccggc agaagggatg
    24001 accgtgcgtg aactggtggc gattggtcgt tacccgtggc atggcgcact ggggcgcttt
    24061 ggtgcggcag atcgcgaaaa ggtcgaagag gcgatttcgc tggttggctt aaaaccgctg
    24121 gcgcatcggc tggtcgatag tctctctggc ggcgaacgtc agcgggcgtg gatcgctatg
    24181 ctggtggcgc aggatagccg ttgtctgttg ctcgacgaac cgacctcggc gctggatatc
    24241 gcccaccagg ttgatgtgct ggcgctggtg catcgtttaa gtcaggaacg tggtctgacg
    24301 gtcattgccg tgctgcacga tattaatatg gctgcgcgct actgtgatta tctggtcgcc
    24361 ctgcgcggtg gtgaaatgat tgctcaggga acgcctgcgg aaattatgcg cggcgaaacc
    24421 ctcgaaatga tttatggcat cccgatgggt attctgccgc atccggcggg tgctgcacct
    24481 gtgagttttg tttattgatg agcggcttac ctcttatttc gcgccgccga ctgttaacgg
    24541 cgatggcgct ttctccgttg ttatggcaga tgaataccgc ccacgcggcg attattgatc
    24601 ccaatcgtat tgtggcgctg gagtggttgc cggtggaatt actgctggcg ctcggcatcg
    24661 tgccttacgg cgtggcggac accatcaact atcgcctgtg ggtcagcgaa ccgccattgc
    24721 cggactcagt gattgacgtt ggtttgcgta cagaacccaa ccttgaactg ctgaccgaaa
    24781 tgaaaccgtc gtttatgatc tggtcggcag gatatggccc ttcaccagaa atgctggcac
    24841 gtattgcgcc ggggcgcgga tttaacttca gcgacggcaa acagccgctg gcgatggcgc
    24901 gtaaatcact gacggaaatg gcagatttac ttaacctgca aagcgcagca gaaacgcatt
    24961 tagcgcaata tgaagacttt atccgcagca tgaaaccccg ctttgtgaag cgtggtgcac
    25021 gcccgttatt gctgacgacg cttatcgatc cgcgccatat gttggtcttc ggtccaaaca
    25081 gcttgttcca ggaaattctt gatgagtacg gcatcccaaa tgcctggcaa ggggaaacca
    25141 acttctgggg cagtaccgcc gtcagtatcg atcgtctggc ggcgtataaa gacgttgatg
    25201 tgctctgttt tgatcacgac aacagcaaag acatggatgc gctaatggca accccgctgt
    25261 ggcaggccat gccgtttgtc cgtgcaggac gttttcagcg cgttccggcg gtctggttct
    25321 atggcgcgac gctttcggca atgcactttg tgcgtattct ggataacgcc atcggaggta
    25381 aagcgtgagt aaacgaattg cgctttttcc ggcgttattg ctggcgctgc tggtgattgt
    25441 cgctacggcg ctcacctgga tgaacttctc gcaggcgttg ccgcgcagcc agtgggcgca
    25501 ggccgcctgg tcgccggata ttgacgtcat cgagcagatg atttttcact acagcttgtt
    25561 gccgcgtctg gcgatttcgc tgctggtggg cgcgggcctg gggctggtgg gcgtgctgtt
    25621 tcagcaagtg ctgcgtaacc cgctggcgga accgacgacg ttgggcgttg caacaggcgc
    25681 gcaactgggg attaccgtca ctacgctctg ggcgatccca ggtgctatgg cgagccagtt
    25741 tgctgcgctg gcaggggctt gcgttgttgg cttaattgtc tttggcgtcg cgtgggggaa
    25801 acggctgtcg ccggtaacgc tgatcctcgc ggggctggta gtgagccttt attgtggggc
    25861 aatcaatcag ttactggtta tcttccatca cgaccagcta caaagcatgt tcctgtggag
    25921 caccggaacg ctgacgcaaa ccgactgggg cggcgttgag cgtttatggc cgcagctgct
    25981 gggcggcgtg atgctgacgt tattgctact tcgcccgtta accctgatgg ggcttgatga
    26041 tggcgtggcg cgcaatctcg gtctggcctt gtcgctggcg cgtctggcgg cgctgtcgct
    26101 ggcgatagtc atcagtgcgc tgctggtgaa tgcagtgggg attatcggct ttatcgggtt
    26161 gttcgcgccg ctgctggcaa aaatgctggg ggcgcggcgt ctgctgccac ggttaatgct
    26221 ggcgtcgctg attggtgcgt taattctgtg gctttctgat caaatcatcc tctggctgac
    26281 tcgcgtgtgg atggaagttt ccaccggttc ggtcactgcg ttgatcggtg cgccgctgct
    26341 actgtggctg ctgccgcgtt tacgcagcat tagcgcgccg gatatgaagg tcaacgatcg
    26401 tgtcgcggct gaacgccaac atgtgctggc gtttgccctc gcgggcggcg tgctgctgtt
    26461 gatggctgtg gtagtggcgc tgtcgtttgg tcgtgatgcg cacggctgga cgtgggcgag
    26521 cggggcgttg ctcgaggatt taatgccctg gcgctggccg cgaattatgg cggcgctgtt
    26581 tgcgggcgtc atgctggcgg tggcgggctg tattattcag cggctgaccg gaaacccgat
    26641 ggcaagcccg gaagtgctgg ggattagctc cggcgcggcg tttggcgtgg tgttgatgct
    26701 gtttctggtg ccgggtaatg cctttggctg gctgttacct gcaggcagtc tcggcgcggc
    26761 ggtgacgctg ttgatcatta tgatcgccgc cggacgcggt ggattttccc cacaccgcat
    26821 gttactggcg gggatggcgt taagtaccgc gttcaccatg cttttgatga tgttgcaggc
    26881 aagtggtgac ccgcgaatgg cgcaagtgct gacctagatc tccggttcga cctacaacgc
    26941 gaccgatgcg caggtctggc gcacgggaat tgtgatggta attttgctgg cgattaaccc
    27001 gctgtgccgc cgctggctga ccattttacc gctgggcggt gataccgcac gagccgtagg
    27061 aatggcgctg acgccgacgc gaattgcgct gctgctatta gcggcttgcc tgacggcgac
    27121 cgcgacgatg actattggac cgttgagttt tgttggttta atggcaccgc atattgcgcg
    27181 gatgatgggt tttcgacgga cgatgccaca catcgtaatt tcggcgctgg tgggtggttt
    27241 actgctggtg ttcgctgact ggtgtgggcg gatggtattg tttccattcc agatcccggc
    27301 ggggctgctg tcaaccttta tcggcgcgcc atattttatc tatttgttga gaaagcagag
    27361 ccgttaatag aatgcctcgc ccggtgggcg aggcaaaatc atcacaactt cgcaaacacc
    27421 cgacgtgcag catcgatggt gttattgata tcttccatgc tgtgcgccac ggacataaag
    27481 cccgcttcaa acgctgacgg tgccagataa acaccttcgt ccagcatcat atggaagaaa
    27541 cgcttaaagc gttccacgtc acaggccatc acatcctgat agcacgtcac ggactcggcg
    27601 tcggtaaaga aaataccgaa catgccgcca acgtggttta ccaccagcgg aattccggct
    27661 tcttctgccg cttccagcag accttctgcc agacgtgttg tcagctcatc cagcgtctcg
    27721 tgaacgcccg gctgtgcgac ttcattcaga caggcgaaac cagctgccat cgcaatcggg
    27781 ttaccggaaa gcgtacccgc ctgatagacc ggaccagtcg gggccagcgc atccattaca
    27841 tcacgacgac caccgaatgc gcctaccggc attccaccgc cgatgatttt gccgaggcag
    27901 gttaaatctg gcactacgcc gtaataatcc tgtgcgccag ctagcgctac gcggaaaccg
    27961 gtcatcactt catcgatgat cagcaacgcg ccaaattcgt cacacagcgc acgcagacct
    28021 ggcaggaact ctggcagcgg tggaacgcag ttcatattgc ctgccaccgg ctcgacgata
    28081 atacaggcaa tctcatgcgg gtactgttcg aacgcggcgc gtacagaggc cagatcgtta
    28141 taagtacagg ttaaggtatg tttggcgaaa tctgccggaa cacccggcga gtttggctgg
    28201 cctaacgtga gtgcgccaga accggctttc accagcaggc agtcagcgtg accgtggtaa
    28261 caaccttcaa atttaatgat tttgtcgcga ccggtaaaac cacgggccag gcggattgcg
    28321 ctcatggtcg cttcagtgcc ggagttcacc atgcgcacca tatccatggt cgggaccagt
    28381 tcggtcacca gttgcgccat tttcacttcc atttcggtcg gtgcaccaaa gcttaaacca
    28441 cgctcggcgg cttcaatcac ggcattgcgg atcgccggat ggttatggcc cagcaccatc
    28501 ggcccccagg aaccgacata atcgatatag gctttgccat caacatcgta cagataagcg
    28561 ccgtccgctt tttcgataaa cagtggagtg ccgcccacgc cagtaaaggc gcgaacaggg
    28621 gagttcacac cgccagggat cagctcgcgc gctgcgctgt aaagattttc agacttactc
    28681 atggagggtt cctgattcgt agaaaaagtg aatggctgct attctatgtt attcataaca
    28741 agttaaatac tcgtcaaaca tcaggctgct tgtactggtg caatcctgat ttcttagagt
    28801 ataaaagttt tgtgcatttg aaacattacg ctttgcaaag gattttcatg gaacgtgcga
    28861 gtaaaatgcc gtcatcttat ttgtatgacc aataagtgat cattggatga aaactgatac
    28921 tccctcttta gaaacaccgc aggccgcgcg cctgcgacgc agacaactga ttcgccaact
    28981 tcttgagcgc gataaaaccc cgttagccat tttgtttatg gcggcagtcg tcggcacgct
    29041 tgtcgggctg gctgcggttg cttttgacaa aggtgtcgcc tggttgcaga accaacgtat
    29101 gggggcactg gtacatactg ctgataatta tccgctactg ttaaccgtcg cttttctctg
    29161 ttcggcggtg ctggcgatgt tcggctactt tttggtgcgc aaatacgcgc cggaagcagg
    29221 tggttcgggg atcccggaaa ttgaaggggc gctggaagat caacgtcccg ttcgctggtg
    29281 gcgtgtgttg ccggtgaaat tctttggcgg gctgggaaca ctcggcggag gcatggtgct
    29341 gggccgcgaa gggccaaccg tacagatcgg cggcaatatc ggtcgcatgg tgcttgatgt
    29401 tttccgcctg aaaggtgatg aagcgcgcca tacgctgctg gcaaccggtg ctgcggcggg
    29461 gctggctgcg gcctttaacg ccccgctggc gggtattttg tttattatcg aagagatgcg
    29521 tccgcagttt cgctatacgt taatttcgat taaagcggta tttattggcg tcattatgtc
    29581 gaccattatg taccggattt ttaatcatga agttgcgttg attgacgtcg gtaaactttc
    29641 tgacgcgccg cttaatacgc tgtggcttta tctgatcctc ggtattattt ttggcatttt
    29701 cggccctatt tttaataaat gggtgctggg gatgcaggat ttgctgcacc gtgtgcacgg
    29761 cggcaatatt accaaatggg tactgatggg cggtgcaatt ggcggtctgt gtggattgct
    29821 ggggtttgtg gcaccagcaa cgtcgggcgg cggttttaac ctgattccta tcgctaccgc
    29881 ggggaatttc agcatgggaa tgctggtgtt tatcttcgtc gcgcgggtca ttaccacctt
    29941 actctgcttc tcttccggcg cgccgggcgg tatttttgcc ccgatgctgg cgctgggtac
    30001 tgtgctggga accgctttcg gaatggttgc cgttgagctg tttccgcaat atcaccttga
    30061 ggcggggaca tttgctattg ccggaatggg ggcattactg gcggcatcta ttcgcgcgcc
    30121 gttaacgggg atcattctgg ttctggaaat gaccgataac taccagctca ttttgccaat
    30181 gattattacc ggtcttggcg caacactatt agcgcaattt accggcggga aaccgctata
    30241 ctcggcgatt cttgcgcgca cgctggcgaa acaggaagct gagcaactgg cgcgaagcaa
    30301 ggccgcatca gccagcgaga atacttgaac gaaataccag ggtattagat aatggcgatt
    30361 attattgggt tagaatttgc ccaattgccg atgtcgtttg gagcaaaata tgagtgatga
    30421 cgtagcactg ccgctggagt ttaccgacgc agcagccaac aaagttaaaa gcctgatcgc
    30481 tgacgaagat aacccgaatc tgaaattacg cgtgtatatt accggtggcg gttgcagcgg
    30541 cttccagtat ggtttcacct ttgatgatca ggtgaacgaa ggcgatatga ccatcgaaaa
    30601 acagggcgtt ggcctggtgg ttgatccgat gagcctgcaa tatctggtcg gcggttccgt
    30661 tgactatacc gaaggtctgg aaggttctcg tttcatcgtg accaacccga acgcgaaaag
    30721 cacctgcggt tgcggttctt cctttagcat ctaatctgtt gttcatgtgc cggataaact
    30781 atccggcaca tcttctctct tcaacgccca ttctcatcca gcgcaaacgt cggtagctta
    30841 agatgccaac gaatagccgc cagccgaatc aatagcgtca cgaccatgcc catcatactg
    30901 gctgtttcca gtggtacgga aaatgtgtaa taagccgtag cgtggacaat accgccgata
    30961 atacaggctg ttgcgtagat ttctgtacgt aaaatcatgg ggatctcgcg ggcaagtaca
    31021 tcacgaatga tcccgccgcc aacgccagta atgacgccca tacaaaccgc gattaacgga
    31081 ccggcttccg cattaaaggc tttattcacg ccaatgccga caaacactgc cagaccaacg
    31141 gcgtccagca ccggcaacat ccattttggt aagcgtcttg gctggcgcac cagcacgatg
    31201 gtcagcatgc tggtgaccat tgcaacgacc agatcggtgg gatctttcac ccaaaatacc
    31261 gggccgtgat ccagcgccat gtcgcgaatt gttccgccgc ctactgcggt aaccacgccc
    31321 aataccagaa caccaaaagg gtccatacgc aattttccgg ctaacaaaac gccagagata
    31381 gcaaataccg ctgtgccgac tatatccagc caatagacga gcattgttca atccccactg
    31441 agcacctgaa aaggtcaggc attaatctac ctgtgaaagc gcattacaga gctgttgtgc
    31501 agcgaggata atacgtgggc ttgcacgttc aaaccagtca ctcgtgagag gaataacggg
    31561 aattttgagc tgttcacccc agtattgttt gattttagga atttggtccg gtccgcctgt
    31621 aatgacaatc gcctgtggcg cgcgtgctaa cacctgttcg cggctaactt gcggccaggg
    31681 aacccggctg tctttaaaga tgttttctcc gccacaaact tcgagtacct ggttttgaat
    31741 cgactctttt ccactagtaa atggcggatt aatgccgaat tgcagaaaaa cacgtttttt
    31801 aggtttatca gcatattgcg ctttcaattg cgcgtactgg tccagcaggg attgcgcggc
    31861 ttgttcggcc ttatccggtt gcggactcca ggggaccagt tgacgtaacg cattggcaat
    31921 ttgttcaatg cttgtcgcat cgacccacat cacttttatt cccagagaag ccagctggtc
    31981 aacctgccgc tcggcattac ctccacgcca ggcaatcacc agatcgggtt tcagcgccac
    32041 aatgcgttcc agattcatcc cctgccaggt ggaaacctgc tcaatttttt gcgcttgtgg
    32101 aggatagtcg gaatagctgc tgaccccaac cggcgtgatc ccggcggcaa aggcaagttc
    32161 agtgttggcg ggagaaagcg tgatgacgcg cggcgcggcg ttgagccaca gcggcgcgag
    32221 aaaagacagg gcgaccagcg ccctgaacag tgacttagcc atgtgccagt ttctgcacca
    32281 gtgactcaac catcaggctg gactgtttag cggcaacagc caggaactca tcgaagctaa
    32341 gatgagactg ttgatcggcc acgtcggaaa tggcgcgtac gacaacaaac gggacgttga
    32401 aattgtggca gacatgggcg attgccgtcg cttccatctc tacagcaatg gcctgtggga
    32461 agttgtggcg gattttcgcc agaccaacag aaccattgat gaaagcgtcg ccgctgacaa
    32521 tcaggccacg tacagcgttt agattcagtt cggcaatgca ggcctcagcg gcagcgatca
    32581 gtttatcgtc agctttaaag cctgccgggc agcctggtaa ctggccgtat tcataaccaa
    32641 atgccgtgac atccgcgtcg tgataacgcg cttcgtccga gacaacgata tcgcccactt
    32701 tcagtgttgg tgccaggcca ccggcagaac cggtgttaat aatcacatct ggcttgcagt
    32761 gttccaacag caaagtggca cccagcgccg cagcgacttt accgatgccc gatttcagaa
    32821 gcgcaacctc ggttccattc agttggccgg tatagatttc gcaaccgccg agactgatag
    32881 tttgacggtt ttcgattttg tcacgcagta gcgtaacttc ttcttccatt gcaccaatga
    32941 tgccgatttt catagattta ctcgcgataa gcccgatttg aaggcatagt ttaccatgcg
    33001 cttacgggga agcgtatttc tcacgcggga gaggacatgg cacagattga tttccgaaaa
    33061 aaaataaact ggcatcgtcg ttaccgttca ccgcagggcg ttaaaaccga acatgagatc
    33121 ctacggatct tcgagagcga tcgcgggcgt atcatcaact ctccggcaat tcgtcgtctg
    33181 caacaaaaaa cccaggtttt tccactggag cgcaatgccg ccgtgcgcac gcgtcttacc
    33241 cactcgatgg aagtccagca ggtggggcgc tacatcgcca aagaaatttt aagccgtctg
    33301 aaagagctta aattactgga agcatacggc ctggatgaac tgaccggccc ctttgaaagc
    33361 attgttgaga tgtcatgcct tatgcacgat atcggcaatc cgccgtttgg tcattttggc
    33421 gaagcggcga taaatgactg gtttcgccag cgtttgcacc cggaagatgc cgaaagccag
    33481 cctctgactg acgatcgctg cagcgtggca gcactacgtt tacgggacgg ggaagaaccg
    33541 cttaacgagc tgcggcgcaa aatacgtcag gacttatgtc attttgaggg gaatgcacaa
    33601 ggcattcgtc tggtgcatac attgatgcgg atgaatctca cctgggcaca ggttggcggt
    33661 attttaaaat atactcgtcc ggcgtggtgg cgtggtgaaa cgcctgagac acatcactat
    33721 ttaatgaaaa agccgggtta ttatctttct gaggaagcct atattgcccg gttgcgtaaa
    33781 gaacttaatt tggcgcttta cagtcgtttt ccattaacgt ggattatgga agctgccgac
    33841 gacatctcct attgtgtggc agaccttgaa gatgcggtag agaaaagaat atttaccgtt
    33901 gagcagcttt atcatcattt gcacgaagcg tggggccagc atgagaaagg ttcgctctat
    33961 tcgctggtgg ttgaaaatgc ctgggaaaaa tcacgctcaa atagtttaag ccgcagtacg
    34021 gaagatcagt tttttatgta tttacgggta aacaccctaa ataaactggt accctacgcg
    34081 gcacaacgat ttattgataa tctgcctgcg attttcgccg gaacgtttaa tcatgcatta
    34141 ttggaagatg ccagcgaatg cagtgatctt ctaaagctat ataaaaatgt cgctgtaaaa
    34201 catgtattta gccatccaga tgtcgagcag cttgaattgc agggctatcg ggtcattagc
    34261 ggattattag agatttatcg ccctttatta agcctgtcgt tatcagactt tactgaactg
    34321 gtagaaaaag aacgggtgaa acgtttccct attgaatcgc gcttattcaa caaactctcg
    34381 acgcgccatc ggctggccta tgtcgaggct gtcagtaaat taccgtcaga ttctcctgag
    34441 tttccgctat gggaatatta ttaccgttgc cgcctgctgc aggattatat cagcggtatg
    34501 accgacctct atgcgtggga tgaataccga cgtctgatgg ccgtagaaca ataaccaggc
    34561 ttttgtaaag acgaacaata aatttttacc ttttgcagaa actttagttc ggaacttcgc
    34621 gctataaaac gaatctgaag aacacagcaa ttttgcgtta tctgttaatc gagactgaaa
    34681 tacatgaaaa aaaccacatt agcactgagt gcactggctc tgagtttagg tttggcgtta
    34741 tctccgctct ctgcaacggc ggctgagact tcttcagcaa cgacagccca gcagatgcca
    34801 agccttgcac cgatgctcga aaaggtgatg ccttcagtgg tcagcattaa cgtagaaggt
    34861 agcacaaccg ttaatacgcc gcgtatgccg cgtaatttcc agcagttctt cggtgatgat
    34921 tctccgttct gccaggaagg ttctccgttc cagagctctc cgttctgcca ggggggccag
    34981 ggcggtaatg gtggcggcca gcaacagaaa ttcatggcgc tgggttccgg cgtcatcatt
    35041 gatgccgata aaggctatgt cgtcaccaac aaccacgttg ttgataacgc gacggtgatt
    35101 aaagtccagc tgagcgatgg ccgtaagttc gacgcgaaga tggttggcaa agatccgcgc
    35161 tctgatatcg cgctgatcca gatccagaac ccgaaaaatc tgaccgcaat taagatggcg
    35221 gattctgatg cactgcgcgt gggtgattac accgtagcaa ttggtaaccc gtttggtctg
    35281 ggcgagacgg taacttccgg gattgtctct gcgctggggc gtagcggcct gaatgctgaa
    35341 aactacgaaa acttcatcca gaccgatgca gcgatcaacc gtggtaactc cggtggtgcg
    35401 ttggttaacc tgaacggcga actgatcggt atcaacaccg cgatcctcgc accggacggc
    35461 ggcaacatcg gtatcggttt tgctatcccg agtaacatgg tgaaaaacct gacctcgcag
    35521 atggtggaat acggccaggt gaaacgcggt gagctgggta ttatggggac tgagctgaac
    35581 tccgaactgg cgaaagcgat gaaagttgac gcccagcgcg gtgctttcgt aagccaggtt
    35641 ctgcctaact cctccgctgc aaaagcgggc attaaagcgg gtgatgtgat cacctcactg
    35701 aacggtaagc caatcagcag ctttgccgcc ctgcgtgctc aggtgggtac tatgccggtg
    35761 ggcagcaaac tgaccctggg cttactgcgc gacggtaagc aggttaacgt gaacctggaa
    35821 ctgcagcaga gcagccagaa tcaggttgat tccagctcca tcttcaacgg cattgaaggc
    35881 gctgagatga gcaacaaagg caaagatcag ggcgtggtag tgaacaacgt gaaaacgggc
    35941 actccggctg cgcagatcgg cctgaaaaaa ggtgatgtga ttattggcgc gaaccagcag
    36001 gcagtgaaaa acatcgctga actgcgtaaa gttctcgaca gcaaaccgtc tgtgctggca
    36061 ctgaacattc agcgcggcga cagcaccatc tacctgttaa tgcagtaatc tccctcaacc
    36121 ccttcctgaa tacgggaagg ggttctcctt acaatctgtg aacttcgcca caactccata
    36181 catcttcatc atcctttggg catttgcaca atgccgtacg ttacgtactt ccttatgcta
    36241 agccgtgcat aacggaggac ttatggctgg ctggcatctt gataccaaaa tggcgcagga
    36301 tatcgtagca cgtaccatgc gcatcatcga taccaatatc aacgtaatgg atgcccgcgg
    36361 gcgaattatc ggcagcggcg atcgtgagcg tattggtgaa ttgcacgaag gagcattgct
    36421 ggtactttca cagggacgag tcgtcgatat cgatgacgcg gtggcacgtc atctgcacgg
    36481 tgtgcggcag gggattaatc taccgttacg gctggaaggt gaaattgtcg gcgtaattgg
    36541 cctgacaggt gaaccagaga atctgcgtaa atatggcgaa ctggtctgca tgacggctga
    36601 aatgatgctg gaacagtcgc ggttgatgca cctgttggcg caggatagcc gtttgcggga
    36661 agaactggtg atgaacctca ttcaggctga ggagaatact cccgcactta ctgaatgggc
    36721 gcaacggctg gggatcgatc tcaatcaacc gcgagtggtg gctattgttg aggtcgacag
    36781 cggtcagctt ggcgtggaca gcgcaatggc ggagttacag caactgcaaa atgcgctgac
    36841 tacgcccgag cgtaataatc tggtggcgat tgtctcgcta accgaaatgg tggtgttgaa
    36901 accggcgttg aactcttttg ggcgctggga cgcagaagat catcgtaaga gagttgaaca
    36961 actgattacc cgcatgaaag agtatggcca gttgcgtttt cgcgtttcgc tgggcaacta
    37021 ttttaccggt cctggcagta ttgcccgatc ctatcgtacg gcgaaaacga cgatggtagt
    37081 ggggaaacag cggatgccag aaagtcgctg ctatttttat caggatctga tgttacctgt
    37141 gttactcgac agtttgcgtg gcgactggca ggccaacgaa ctggcgcgac cgctggcgcg
    37201 gctgaaagcg atggataata acggcttgct gcgacgtacg ctggcggcgt ggtttcgcca
    37261 taacgtgcag ccgctggcaa cgtcaaaggc gctgtttatt catcgtaata ccctggagta
    37321 tcggcttaat cgtatatcgg aactcacagg cttagatctg gggaattttg atgacaggtt
    37381 attgttgtat gtggcgttac aactggatga acagcgatag ggtaaaaata gtaaatgccg
    37441 ggaaatatgc tcgtcttaat tcaagttgca tgtacggcga ctcgaattat tcagcgtata
    37501 ctttacccgg ctggcagaat ggcaatatgc aaaattattt acgtgtcagc ttttctaaat
    37561 ctgcttctat ctcgctgatt ttattggtca ccactgactc caggtgacgc aagtcgtcga
    37621 gaattttacg cttaaggtca acttcgctac gatcacgctg gcagatttga tcaagctcat
    37681 caatgatata acgaagatta gggctgattt cctgaacttc tttatatccc tggccaatac
    37741 catcggcgac caccgttttg cgctgacgcg gatatttaaa cttcacactc ttggcgaaaa
    37801 actcgccttt atctttctga aaatagattt tcaaaatatc gttattggct tcctgccgga
    37861 ggctgtaacg atcaatttcc tcaggattgg taatgcccaa acttttcaga ttgtcgtaca
    37921 tagttgtacc tcaaaagagt cagtaattgt gcttatctta gcatttcgcc tggcggttta
    37981 gctcgacgga gatcgcagga agccttttct tccagacttt tatcatggcc taaaaatcaa
    38041 aaaattacct gctttattct gataagagaa ttcgctttct acaccagttc caggattgtc
    38101 acttccgggc ggcaattcag acgcaagcca tacaaacttc cgacaccccg agtcgtgtaa
    38161 atttgtcgct caccaaaggc atttaaaccg gcggcgtagc gtttatcttc tatgggcgca
    38221 aaaggctcac caaccaacgg tatgcgtaac tgcccgccgt gggtgtgacc acagagcatc
    38281 agatcccact tttcattacg catgacttct ttgctgtcgg gattatgcgc cagcaccagt
    38341 cgtggcagat ttgcttcgct ggcgggaggc ggtttgcatt gtccggtcca taaatcaccc
    38401 gtgccaacca gttcaaattg ctgcttttga gtggtgatta gcatggcttc gttaaataac
    38461 accgtgatcc ccgccgattt taatgcctcg ccaattaagc gatttttctc tgtaccaaca
    38521 gggcgatcgt ggttgccgta acaggcaaat gtcggcgcac attcggcaag ggaggagagt
    38581 acgtcactaa acgccgaaaa attcagctgc atatcaaata atacgtaatc gccgcccagc
    38641 aatatcaaat cgggcttttg tgctacgcca agagcaattg cgtcagaaat caggcttaaa
    38701 ggaacaaaac gagagtaatg gagatctgcc agaaaaagga ttttgaaagg agcagcgttg
    38761 tctttaaaaa aggcgaggcg gtgacggatt aattcaagcc agccaggttc ccaataatgc
    38821 atatagctga ggcttgatcc ggcagctaac gtcgcagcgg ctgcctgtaa aaaacgcctg
    38881 cgtgtgatca tcatccatcc ctgcaaaaaa tcgggcagcg tcatgctgcc cgtgtactga
    38941 tttttagtcg atggtacgca gcagttcgtt aatgccgact ttgccgcgag ttttcgcgtc
    39001 aactttctta acgataactg cacaataaag gctgtatttg ccatctttcg acggcaggtt
    39061 gccagaaaca accaccgaac cagccggaac gcgaccgtag tggatttcgc cggtttcacg
    39121 gtcgtaaata cgggtgctct gaccaatgta tacgcccata gagatgacgg aaccttcttc
    39181 gacaatcacc ccttcaacca cttcagagcg tgcaccgatg aagcaattat cttcaataat
    39241 ggtcgggtta gcctgcagcg gctccagcac gccgccgatg cctacaccgc cagacagatg
    39301 gacgttttta ccaatttgtg cacaggaacc gacagtggcc caggtatcaa ccatagtgcc
    39361 ttcatcaaca tatgcgccga tgttgacgta agacggcatc agcacggtgt tacgggcaat
    39421 aaatgcaccc tgacgtaccg ccgctggtgg cacaacgcgg aagccttctt tctgaaaacg
    39481 cgcttcgtcg tagtcggcga atttcatcgg cactttatcg aagtagcggc tttctgcccc
    39541 ttcgatcacc tgattatcat taatacggaa agagagcagc accgcttttt tcaaccactg
    39601 atgcgtcacc cactgaccgt caattttttc cgctacacgc agtgcgccgg aatccagcag
    39661 ggcgatcacc tgatttaccg cttcgcgggt tacggtgtct gcattggctg gcgtgatctc
    39721 ggcacggcgt tcaaaagcgg tttcaataat gttctgtaac tgctgcattg ttaaactctt
    39781 ttcatatcag taaacacatc accctttatc gtttggattg agggcctctg tcaaccgctg
    39841 atgcacttcc tgctgcaact cgttattaag cgcacgccgg tcagcggtgg caattatgaa
    39901 taaatcttct actcgctcgc caatggttgt aattcgggcg ccatgaagcg aaattcccag
    39961 atcggcaaaa attttcccga ctcgcgccag cagtccaggt tggtcgaggg cgatcagttc
    40021 gaggaacgat ttgcggtcgg tatgggtcgg caaaaacgtc acttcggttt caacagtaaa
    40081 atggcgcaat ttggcgggtt ggcgacgggg ctgcggtggc tgccagctac tttgcgtcag
    40141 cacctgctcc agaccaaacc gaataacctc atgacgatct gcggatagcg ggctaccatc
    40201 gggttccagc acgatgaagg tatccatcgc cataccgtcg cgagtggtga aaatttgtgc
    40261 gtcgtgaaca cttaaattgc ggcggtctaa ttcggcacag acggcggcaa acagataggg
    40321 gcggtccggg ctccagataa aaatctcggt gcctccacgc gtagcctgcg gactaagcaa
    40381 taccagcggt ttgcttaaat catgctgtaa taaatggcgc gcatgccagg ccagttgatt
    40441 tgggctatgg cggacaaaat agttagcacg acagcgtgac caaatttggt gcagcgcctc
    40501 ttcgtcgatg ttatccatgc gcagtagtgc cagcgcctgg agttggtgat ggcgaacccg
    40561 ttcgcgcata tccggcgtgt tttgcattcc gcgtcgtagc tgcttttcgg tggcgaaata
    40621 gagttcgcgc aacagacttt gcttccagct attccacagc gtttcgttgg tggcgcatat
    40681 atcggccact gtcaggcata ccagataacg aagtcggttt tctgtttgca cttcttcggc
    40741 aaactgcttg atcacttccg ggtcctgaat atcgcggcgt tgggcggtca ccgacatcag
    40801 caagtgctgg cgaaccagcc acgccaccag ctgtgtttcg cgcgagttaa gcccgtggag
    40861 ttcggcaaaa tgcaccacgt cttgtgcacc gagaatggag tggtcgccgc cacgcccttt
    40921 ggcgatatcg tgaaacagcg cggcgatgaa aatcagctca gttgacggca ggcgcggcca
    40981 gacgtctaca cacaacggat ggcgctggcg cgtttcttca ctggcaaaac tctccagttt
    41041 cagcatcacg cgaatagtat gttcatccac cgtgtaggcg tggaacaggt caaactgcat
    41101 ctgcccgacg atatgcgacc attggggcat atacgcgccg agcacgctat gacgatgcat
    41161 cggtaatagt ccgcgacgca ccgcaccagg gtgacgcaga atgctcaaaa acagttttcg
    41221 cgcttccgga atattacaca gcggttgttg caaatggcga cgggcgtggc gtaactggcg
    41281 cagcgtggta gagtaaatgc cggtgatcgc actgttgcgc accatggtgt agaacatacg
    41341 caagatggct tccggctggc gcataaatag tgtttcatca cgcaggtcga tcagcgttcc
    41401 gcgtagctga aactcatcgt cgattggacg tggtttttcg tcggcgggaa gggcgaggat
    41461 ggcttcatcg aacagttgca gcagcatctg gttgagttca ctgacgcggc gtgtaacgcg
    41521 gaagtaatcc ttcatcatcc gctcgaccgg ttcgttacct tcgccactgt aattcagacg
    41581 ctgggcgacg ctaagctggc gatcaaacag caggcgatta tcgtaacggc tgacgaccag
    41641 atgcagggca aagcgaatac gccacaatat atgcagacat tcgtttaatt ccgctcgctc
    41701 cgctgaggtt aagaagccaa acccgaccat ttcatccagc gatgttgcgc caaaatgacg
    41761 gcgggccacc cattgcagag tgtgaatatc gcgcaagccg ccaggactac ttttgatgtc
    41821 tggttcaagg ttgtagctgg tgccgtggta acgctgatgg cgctggttct gttcttcaac
    41881 tttcgctgcg tagaacttgt cggaaggcca gaatccttcg ctaaaaatat gtttttgcag
    41941 ttcgaggaag agcgctacat cgccaattaa taagcgggat tcgattaaat tggtggcgac
    42001 ggttaaatcc gataaccctt ccagcatgca ctcttcaagc gtgcgcacgc tatgaccgac
    42061 ttccagcttt acatcccaga gcagcgttaa cagctcgccc actttttgcg cctgatcatc
    42121 cgggagcttt ttacggctta aaatcagtaa atcgatgtcg gaaagtggat gcagctcgcc
    42181 acgaccataa ccaccgacgg caaccaatgc caggtcggca atctggctga atcccgcttc
    42241 aatccataat cgttgcagga gttggtcgat aaactcggtg cgtgcctcaa tcaactgttc
    42301 tgcggagatc ccattgtcaa aggcatcacc cagccaacgc tggaaagtat cgatatgggc
    42361 ttttatccca cagacggtta attcatcacg gggccaggcg catggatttt gcggttgacc
    42421 gggcagggtg gggagagcgg tgtttgcgta ctgttctgga agggtattca ttgtgcgcca
    42481 cccataagat taaattatcg cattaaaaaa gccggcattc gccggcttca tcttattcgt
    42541 cgtgcgagat tatcgccggg atggtgtcat ccttgcgtag cgtcagaatt tcgcagccgt
    42601 tatcagtcac cacaatagta tgctcatatt gtgcagacaa gctgcgatct ttggttttta
    42661 ccgtccagcc atctttcatg gtgcggatct cttttttacc cgcgttgacc attggctcga
    42721 tggtgaacgt catcccaggt ttcagtacga cgttggtttc acgggagtca tagtgcagca
    42781 cctgcggttc ttcgtggaag ccgcgaccaa taccgtgtcc acaatattca cgaacgacgg
    42841 agaagccttc tgcttcgaca aatttctgaa ttgccgcacc gatttcgcgc agattaatgc
    42901 caggttttac catgcgtagc gccaggtaca ggctttcttg cgtgatgcgg cacagacgtt
    42961 cgcccatgat ggtcggctta ccgacgataa acattttcga ggtatcgccg tggaaaccat
    43021 ctttgattac ggtgacatca atgttaacga tatcgccatc tttcagcagc ttggcgtcgt
    43081 ccgggatacc gtggcacacc acttcattaa tagaaatgca aacggatttc ggatagccgt
    43141 gatagccgag gcaggcagaa accgcgtgtt gttcattaac aatgtaatca ttacagatgc
    43201 gatccagctc gccggtgctg acgcccggtt taacatacgg ttcgatcatc tccagcactt
    43261 cggcagccag tcggccagcg acgcgcattt tttcgatatc ttctggggtc ttgattgaga
    43321 tagccattaa ttctgtccat cagcgtcggt gataccgaca atatatatgt aagtgccgtc
    43381 aatggtatca cacccgggca aattgagaat cattctgaat ttcgccaaac gtgccactga
    43441 aggttttcta taatagaaaa ttcaacgtct gatgctgtac acagcgccaa caattattgg
    43501 tgtccacgac gtatttgtgg tataaagcgc gccggacttc cgatccattt cgtatacaca
    43561 gactggacgg aagcgacaat ctcactttgt gtaacaacac acacgtatcg gcacatattc
    43621 cggggtgccc tttggggtcg gtaatatggg atacgtggag gcataacccc aacttttata
    43681 tagaggtttt aatcatggca actgtttcca tgcgcgacat gctcaaggct ggtgttcact
    43741 tcggtcacca gacccgttac tggaacccga aaatgaagcc gttcatcttc ggtgcgcgta
    43801 acaaagttca catcatcaac cttgagaaaa ctgtaccgat gttcaacgaa gctctggctg
    43861 aactgaacaa gattgcttct cgcaaaggta aaatcctttt cgttggtact aaacgcgctg
    43921 caagcgaagc ggtgaaagac gctgctctga gctgcgacca gttcttcgtg aaccatcgct
    43981 ggctgggcgg tatgctgact aactggaaaa ccgttcgtca gtccatcaaa cgtctgaaag
    44041 acctggaaac tcagtctcag gacggtactt tcgacaagct gaccaagaaa gaagcgctga
    44101 tgcgcactcg tgagctggag aaactggaaa acagcctggg cggtatcaaa gacatgggcg
    44161 gtctgccgga cgctctgttt gtaatcgatg ctgaccacga acacattgct atcaaagaag
    44221 caaacaacct gggtattccg gtatttgcta tcgttgatac caactctgat ccggacggtg
    44281 ttgacttcgt tatcccgggt aacgacgacg caatccgtgc tgtgaccctg tacctgggcg
    44341 ctgttgctgc aaccgtacgt gaaggccgtt ctcaggatct ggcttcccag gcggaagaaa
    44401 gcttcgtaga agctgagtaa taaggcttga taactccccc aaaatagttc gagttgcaga
    44461 aaggcggcaa gctcgagaat tcccgggagc ttacatcagt aagtgaccgg gatgagcgag
    44521 cgaagataac gcatctgcgg cgcgaaatat gaagggggag agcccttata gaccaggtag
    44581 tacacgtttg gttagggggc ctgcatatgg cccccttttt cacttttata tctgtgcggt
    44641 ttaatgccgg gcagatcaca tctccgagga ttttagaatg gctgaaatta ccgcatccct
    44701 ggtaaaagag ctgcgtgagc gtactggcgc aggcatgatg gattgcaaaa aagcactgac
    44761 tgaagctaac ggcgacatcg agctggcaat cgaaaacatg cgtaagtccg gtgctattaa
    44821 agcagcgaaa aaagcaggca acgttgctgc tgacggcgtg atcaaaacca aaatcgacgg
    44881 caactacggc atcattctgg aagttaactg ccagactgac ttcgttgcaa aagacgctgg
    44941 tttccaggcg ttcgcagaca aagttctgga cgcagctgtt gctggcaaaa tcactgacgt
    45001 tgaagtactg aaagcacagt tcgaagaaga acgtgttgcg ctggtagcga aaattggtga
    45061 aaacatcaac attcgccgcg ttgctgcgct ggaaggcgac gttctgggtt cttatcagca
    45121 cggtgcgcgt atcggtgttc tggttgctgc taaaggcgct gacgaagagc tggttaaaca
    45181 catcgctatg cacgttgctg caagcaagcc agaattcatc aaaccagaag acgtatccgc
    45241 tgaagtggta gaaaaagaat accaggtaca gctggatatc gcgatgcagt ctggtaagcc
    45301 gaaagaaatc gcagagaaaa tggttgaagg ccgcatgaag aaattcaccg gcgaagtttc
    45361 tctgaccggt cagccgttcg ttatggaacc aagcaaaact gttggtcagc tgctgaaaga
    45421 gcataacgct gaagtgactg gcttcatccg cttcgaagtg ggtgaaggca tcgagaaagt
    45481 tgagactgac tttgcagcag aagttgctgc gatgtccaag cagtcttaat tatcgaaaag
    45541 gagccgcctg agggcggctt ctttttgtgc ccatcttgta aattcagcta acccttgtgg
    45601 ggctgcgctg aaaagcgacg tacaatgtcg ctagtattaa ttcatttcaa tcgttgacag
    45661 tctcaggaaa gaaacatggc taccaatgca aaacccgtct ataaacgcat tctgcttaag
    45721 ttgagtggcg aagctctgca gggcactgaa ggcttcggta ttgatgcaag catactggat
    45781 cgcatggctc aggaaatcaa agaactggtt gaactgggta ttcaggttgg tgtggtgatt
    45841 ggtgggggta acctgttccg tggcgctggt ctggcgaaag cgggtatgaa ccgcgttgtg
    45901 ggcgaccaca tggggatgct ggcgaccgta atgaacggcc tggcaatgcg tgatgcactg
    45961 caccgcgcct atgtgaacgc tcgtctgatg tccgctattc cattgaatgg cgtgtgcgac
    46021 agctacagct gggcagaagc tatcagcctg ttgcgcaaca accgtgtggt gatcctctcc
    46081 gccggtacag gtaacccgtt ctttaccacc gactcagcag cttgcctgcg tggtatcgaa
    46141 attgaagccg atgtggtgct gaaagcaacc aaagttgacg gcgtgtttac tgctgatccg
    46201 gcgaaagatc caaccgcaac catgtacgag caactgactt acagcgaagt gctggaaaaa
    46261 gagctgaaag tcatggacct ggcggccttc acgcttgctc gtgaccataa attaccgatt
    46321 cgtgttttca acatgaacaa accgggtgcg ctgcgccgtg tggtaatggg tgaaaaagaa
    46381 gggactttaa tcacggaata attcccgtga tggataaata agggtaagat tccgcgtaag
    46441 tatcgcgggg gcgtaagtct ggttataagg cgttattgtt gcaggcagtt tggtcacggc
    46501 cagcgcgcag caaccggagc gtacaaaagt acgtgaggat ggcgagcact gcccggggcc
    46561 aaaatggcaa ataaaatagc ctaataatcc agacgattac ccgtaatatg tttaatcagg
    46621 gctatactta gcacacttcc actgtgtgtg actgtctggt ctgactgaga caagttttca
    46681 aggattcgta acgtgattag cgatatcaga aaagatgctg aagtacgcat ggataaatgc
    46741 gtagaagcgt tcaaaaccca aatcagcaaa atacgcacgg gtcgtgcttc tcccagcctg
    46801 ctggatggca ttgtcgtgga atattacggc acgccgacgc cgctgcgtca gctggcaagc
    46861 gtaacggtag aagattcccg tactctgaaa atcaacgtgt ttgatcgttc aatgtctccg
    46921 gccgttgaaa aagcgattat ggcgtccgat cttggcctga acccgaactc tgcgggtagc
    46981 gacatccgtg ttccgctgcc gccgctgacg gaagaacgtc gtaaagatct gaccaaaatc
    47041 gttcgtggtg aagcagaaca agcgcgtgtt gcagtacgta atgtgcgtcg tgacgcgaac
    47101 gacaaagtga aagcactgtt gaaagataaa gagatcagcg aagacgacga tcgccgttct
    47161 caggacgatg tacagaaact gactgatgct gcaatcaaga aaattgaagc ggcgctggca
    47221 gacaaagaag cagaactgat gcagttctga tttcttgaac gacaaaaacg ccgctcagta
    47281 gatccttgcg gatcggctgg cggcgttttg ctttttattc tgtctcaact ctggatgttt
    47341 catgaagcaa ctcaccattc tgggctcgac cggctcgatt ggttgcagca cgctggacgt
    47401 ggtgcgccat aatcccgaac acttccgcgt agttgcgctg gtggcaggca aaaatgtcac
    47461 tcgcatggta gaacagtgcc tggaattctc tccccgctat gctgtaatgg atgatgaagc
    47521 gagtgcgaaa cttcttaaaa cgatgctaca gcaacagggt agccgcaccg aagtcttaag
    47581 tgggcaacaa gccgcttgcg atatggcagc gcttgaggat gttgatcagg tgatggcagc
    47641 cattgttggc gctgctgggc tgttacctac gcttgctgcg atccgcgcgg gtaaaaccat
    47701 tttgctggcc aataaagaat cactggttac ctgcggacgt ctgtttatgg acgccgtaaa
    47761 gcagagcaaa gcgcaattgt taccggtcga tagcgaacat aacgccattt ttcagagttt
    47821 accgcaacct atccagcata atctgggata cgctgacctt gagcaaaatg gcgtggtgtc
    47881 cattttactt accgggtctg gtggcccttt ccgtgagacg ccattgcgcg atttggcaac
    47941 aatgacgccc gatcaagcct gccgtcatcc gaactggtcg atgggacgta aaatttctgt
    48001 cgattcggct accatgatga acaaaggtct ggaatacatt gaagcgcgtt ggctgtttaa
    48061 cgccagcgcc agccagatgg aagtgctgat tcacccgcag tcagtgattc actcaatggt
    48121 gcgctatcag gacggcagtg ttctggcgca gctgggggaa ccggatatgc gtacgccaat
    48181 tgcccacacc atggcatggc cgaatcgcgt gaactctggc gtgaagccgc tcgatttttg
    48241 caaactaagt gcgttgacat ttgccgcacc ggattatgat cgttatccat gcctgaaact
    48301 ggcgatggag gcgttcgaac aaggccaggc agcgacgaca gcattgaatg ccgcaaacga
    48361 aatcaccgta gctgcttttc ttgcgcaaca aatccgcttt acggatatcg ccgcgttgaa
    48421 tttatccgta ctggaaaaaa tggatatgcg cgaaccacaa tgtgtggacg atgtgttatc
    48481 tgttgatgcg aacgcgcgtg aagtcgccag aaaagaggtg atgcgtctcg caagctgagg
    48541 ataatccggc tacagagagt cgcgctattt gttagcgtag ggcttcagtg atatagtctg
    48601 cgccatctga tcgtaagtag ttggctttat aaggtcaggt atgccgtggt tttacacggc
    48661 ttttttttgt ataggcttca gtattcctga gtaccgtaaa ccctgtcagg gaataagaaa
    48721 cgcgtgatgt tgtctgctac tcaaccactt agcgaaaaat tgccagcgca tggctgtcgt
    48781 catgttgcga tcattatgga cggcaatggt cgctgggcaa aaaagcaagg gaagattcgt
    48841 gcctttgggc ataaagccgg ggcaaaatcc gtccgccggg ctgtctcttt tgcggccaac
    48901 aacggtattg aggcgttaac gctgtatgcc tttagtagtg aaaactggaa ccgaccagcg
    48961 caggaagtca gtgcgttaat ggaactgttt gtgtgggcgc tcgatagcga agtaaaaagt
    49021 ctgcaccgac ataacgtgcg tctgcgtatt attggcgata ccagtcgctt taactcgcgt
    49081 ttgcaagaac gtattcgtaa atctgaagcg ctaacagccg ggaataccgg tctgacgctg
    49141 aatattgcgg cgaactacgg tggacgttgg gatatagtcc agggagtcag gcaactggct
    49201 gaaaaggtgc agcaaggaaa cctgcaacca gatcagatag atgaagagat gctaaaccag
    49261 catgtctgta tgcatgaact ggcccctgta gatttagtaa ttaggactgg gggggagcat
    49321 cgcattagta actttttgct ttggcaaatt gcctatgccg aactttactt tacagatgtt
    49381 ctctggcccg atttcgatga acaagacttt gaaggggcgt taaatgcctt tgctaatcga
    49441 gagcgtcgtt tcggcggcac cgagcccggt gatgaaacag cctgatgggg gtcgcttttg
    49501 ctgaagtatc gcctgatatc tgcttttgtg ttaatacccg ttgtcatcgc ggcgttgttt
    49561 ctgttgccgc cggtggggtt cgccattgta acgctggtgg tctgcatgct ggcagcgtgg
    49621 gaatggggac agcttagcgg ttttaccact cgttcgcagc gagtatggtt ggcggtgtta
    49681 tgcgggttat tgttggcgct gatgcttttt ctgttgccgg aatatcaccg aaatattcat
    49741 caaccgctgg ttgaaatctc actttgggct tcgctgggtt ggtggattgt cgcgctattg
    49801 ctggtgctgt tttaccctgg ttccgcagca atctggcgta actctaaaac attgcgcctt
    49861 atttttggcg tgctaaccat tgttcccttc ttctggggca tgctggcgtt acgggcctgg
    49921 cactacgacg agaatcatta cagtggcgca atatggctgc tctatgtcat gatcctggta
    49981 tggggcgctg actctggcgc atatatgttt ggcaaattgt ttggtaaaca taagctggca
    50041 ccgaaggttt ctccgggtaa aacctggcaa ggctttatag gtggactcgc gaccgcagcg
    50101 gtaatctcat ggggttatgg catgtgggcg aatctcgacg tcgctcccgt caccttactc
    50161 atttgctcta ttgtcgcagc gttagcctca gtgctcggcg atctgaccga gagtatgttt
    50221 aagcgtgaag caggaattaa ggacagcggt catttaattc caggacacgg tggtatttta
    50281 gatcgtattg atagcctgac ggctgcggta ccggtctttg cttgcttgtt gttactggta
    50341 ttcaggacgc tttaacggaa ggtaatatgc tgagttttct ctgggatttg gcttcgttca
    50401 tcgttgcact gggtgtactt atcaccgtgc atgaatttgg tcatttctgg gttgcccggc
    50461 gttgtggtgt tcgcgttgag cgtttctcaa tagggtttgg taaggcgctc tggcggcgaa
    50521 ctgataagct cggcaccgaa tatgttatcg ccctgatccc gctgggcggt tatgtcaaaa
    50581 tgctggatga gcgcgcagaa ccggtcgttc cggaactccg ccaccatgcc ttcaataata
    50641 aatctgtcgg ccaacgagcg gcgattattg ccgcaggtcc ggttgcaaac ttcatttttg
    50701 ctatctttgc ctactggctg gtttttatta ttggtgtgcc tggcgtacgt ccggtggttg
    50761 gcgaaatagc agccaattcg atagctgcgg aagcacaaat tgcaccaggt acggaactaa
    50821 aagccgtaga tggtatcgaa acgcctgatt gggatgccgt gcgtttgcag ttggtcgata
    50881 aaattggcga tgaaagcacc accattacgg tagcgccatt tggcagcgac caacggcggg
    50941 atgtaaagct cgatttacgt cactgggcgt ttgagcctga taaagaagat ccggtaactt
    51001 cgctggggat tcgtcctcgt gggccgcaaa ttgaacctgt actggaaaat gtgcagccaa
    51061 actcggcggc aagcaaggca ggtttgcaag caggcgacag gatcgttaaa gtcgatggtc
    51121 agcccttaac gcagtgggtg acctttgtga tgcttgtccg ggataacccg ggtaaatcct
    51181 tagcgttaga aatcgaaagg caggggagtc ccttgtcttt gacattaatc ccggagagta
    51241 aaccgggtaa tggtaaagcg attggttttg tcggtattga gccgaaagtc attcctttgc
    51301 cagatgagta taaagttgta cgccagtatg ggccgttcaa cgccatcgtc gaagccacgg
    51361 acaaaacgtg gcagctgatg aagctgacgg tcagtatgct gggaaaattg atcaccggtg
    51421 atgtgaaact gaacaacctc agtgggccga tctctatcgc caagggggct gggatgacag
    51481 cggaactcgg ggttgtttat tacctgccgt ttcttgcgct tattagcgtg aacttaggga
    51541 taattaacct gtttccgttg cccgtacttg acggggggca tctgctgttc cttgcgatcg
    51601 aaaagatcaa gggcggaccg gtatccgagc gggttcaaga cttttgttat cgcattggct
    51661 cgattttgct ggtgctgtta atggggcttg cacttttcaa tgatttctct cggttatgag
    51721 agttagttag gaagaacgca taataacgat ggcgatgaaa aagttgctca tagcgtcgct
    51781 gctgtttagc agcgccaccg tatacggtgc tgaagggttc gtagtgaaag atattcattt
    51841 cgaaggcctt cagcgtgtcg ccgttggtgc ggccctcctc agtatgccgg tgcgcacagg
    51901 cgacacggtt aatgatgaag atatcagtaa taccattcgc gctctgtttg ctaccggcaa
    51961 ctttgaggat gttcgcgtcc ttcgtgatgg tgataccctt ctggttcagg taaaagaacg
    52021 tccgaccatt gccagcatta ctttctccgg taacaaatcg gtgaaagatg acatgctgaa
    52081 gcaaaacctc gaggcttctg gcgtgcgtgt gggcgaatcc ctcgatcgca ccaccattgc
    52141 cgatatcgag aaaggtctgg aagacttcta ctacagcgtc ggtaaatata gcgccagcgt
    52201 taaagctgtc gtgaccccgc tgccgcgcaa ccgtgttgac ctaaaactgg tgttccagga
    52261 aggtgtgtca gctgaaatcc agcaaattaa cattgttggt aaccatgctt tcaccaccga
    52321 cgaactgatc tctcatttcc aactgcgtga cgaagtgccg tggtggaacg tggtaggcga
    52381 tcgtaaatac cagaaacaga aactggcggg cgaccttgaa accctgcgca gttactatct
    52441 ggatcgcggt tatgcccgtt tcaacatcga ctctacccag gtcagtctga cgcctgataa
    52501 aaaaggtatt tacgtcacgg tgaacatcac cgaaggcgat cagtacaagc tttctggcgt
    52561 tgaagtgagc ggcaaccttg ccgggcactc cgctgaaatt gagcagctga ctaagatcga
    52621 gccgggcgag ctgtataacg gcaccaaagt gaccaaaatg gaagatgaca ttaaaaagct
    52681 tcttggtcgc tatggttatg cctatccgcg cgtacagtcg atgcctgaaa ttaacgatgc
    52741 cgacaaaacc gttaaattac gtgtgaacgt tgatgcgggt aaccgtttct acgtgcgtaa
    52801 gatccgtttt gaaggtaacg atacctcgaa agatgccgtc ctgcgtcgcg aaatgcgtca
    52861 gatggaaggt gcatggctgg ggagcgatct ggtcgatcag ggtaaggagc gtctgaatcg
    52921 tctgggcttc tttgaaactg tcgataccga tacccaacgc gttccgggta gcccggacca
    52981 ggttgatgta gtctacaagg tgaaagaacg taacaccggt agcttcaact tcgggattgg
    53041 ttacggtact gaaagtggcg tgagcttcca ggctggtgtg caacaggata actggttagg
    53101 tacgggttat gctgttggta tcaacgggac caaaaacgat taccagacct atgctgaact
    53161 gtcggtaacc aacccgtact tcaccgtaga tggtgtaagc ctcggtggtc gtctcttcta
    53221 taatgacttc caggcagatg acgccgacct gtccgactat accaacaaga gttatggtac
    53281 agacgtgacg ttgggcttcc cgatcaacga atataactcg ctgcgtgcag gtctgggtta
    53341 tgtacataac tccctgtcca acatgcagcc tcaggttgcg atgtggcgtt atctgtactc
    53401 tatgggtgaa catccgagca cctctgatca ggataacagc ttcaaaacgg acgacttcac
    53461 gttcaactat ggttggacct ataacaagct tgaccgtggt tacttcccga cagatggttc
    53521 acgtgtcaac ctgaccggta aagtgaccat tccgggatcg gataacgaat actacaaagt
    53581 gacgttagac acggcgactt atgtgccgat tgatgacgat cacaaatggg ttgttctggg
    53641 tcgtacccgc tggggttatg gtgatggttt aggcggcaaa gagatgccgt tctatgagaa
    53701 cttctatgcc ggtggttcca gcaccgtgcg tggcttccag tccaatacca ttggtccgaa
    53761 agcagtttac ttcccgcatc aggccagtaa ttatgatccg gactatgatt acgaatgtgc
    53821 gactcaggac ggcgcgaaag atctgtgtaa atcggatgat gctgtaggcg gtaacgccat
    53881 ggcggttgcc agcctcgagt tcatcacccc gacgccgttt attagcgata agtatgctaa
    53941 ctcggttcgt acttccttct tctgggatat gggtaccgtt tgggatacaa actgggattc
    54001 cagccaatat tctggatatc cggactatag tgatccaagc aatatccgta tgtctgcggg
    54061 tatcgcatta caatggatgt ccccattggg gccgttggtg ttctcctacg cccagccgtt
    54121 caaaaagtac gatggagaca aggcagaaca gttccagttt aacatcggta aaacctggta
    54181 agtgttctcc acaaaggaat gtagtggtag tgtagcgatg actttaggcg atcaatataa
    54241 gatcgccggg ccacgcaaag aactgcaccc tccggtgcaa atgggatggt aaggagttta
    54301 ttgtgaaaaa gtggttatta gctgcaggtc tcggtttagc actggcaact tctgctcagg
    54361 cggctgacaa aattgcaatc gtcaatatgg gcagcctgtt ccagcaggta gcgcagaaaa
    54421 ccggagtttc taacacgctg gaaaatgagt tcaaaggccg tgccagcgaa ctgcagcgta
    54481 tggaaaccga tctgcaggct aaaatgaaaa agctgcagtc catgaaagcg ggcagcgatc
    54541 gcaccaaact ggaaaaagac gtgatggccc agcgccagac ttttgctcag aaagcgcagg
    54601 cttttgagca ggatcgcgca cgtcgttcca acgaagaacg cggcaaactg gttactcgta
    54661 tccagactgc tgtgaaatcc gttgccaaca gccaggatat cgatctggtt gttgatgcaa
    54721 acgccgttgc ttacaacagc agcgatgtaa aagacatcac tgccgacgta ctgaaacagg
    54781 ttaaataagt aatgccttca attcgactgg ctgatttagc gcagcagttg gatgcagaac
    54841 tacacggtga tggcgatatc gtcatcaccg gcgttgcgtc catgcaatct gcacaaacag
    54901 gtcacatcac gttcatggtt aacccaaaat accgtgagca tttaggcttg tgccaggcgt
    54961 ccgcggttgt catgacccag gacgatcttc ctttcgcgaa aagtgccgcg ctggtagtga
    55021 agaatcccta cctgacttac gcgcgcatgg cgcaaatttt agataccacg ccgcagcccg
    55081 cgcagaacat tgcacccagt gcggtgattg acgcgacggc gaagctgggt aacaacgtat
    55141 cgattggcgc taacgcggtg attgagtccg gcgttgaact gggcgataac gtgattatcg
    55201 gtgccggttg cttcgtaggt aaaaacagca aaatcggtgc aggttcgcgt ctctgggcga
    55261 acgtaaccat ttaccatgag atccagatcg gtcagaattg cctgatccag tccggaacag
    55321 tggtaggcgc agacggcttt ggttatgcca acgatcgtgg taactgggtg aagatcccac
    55381 agattggtcg cgtaattatt ggcgatcgcg tggagatcgg tgcctgcact accattgatc
    55441 gtggcgcgct ggatgacact gttattggca atggtgtgat cattgataac cagtgccaga
    55501 ttgcacataa cgtcgtgatt ggcgacaata cggcagttgc cggtggcgtc attatggcgg
    55561 gcagcctgaa aattggtcgt tactgcatga tcggcggagc cagcgtaatc aacgggcata
    55621 tggaaatatg cgacaaagtg acggttacgg gcatgggtat ggtgatgcgt cccatcactg
    55681 aaccaggcgt ctattcctca ggcattccgc tgcaacccaa caaagtctgg cgcaaaaccg
    55741 ctgcactggt gatgaacatt gatgacatga gcaagcgtct gaaatcgctt gagcgcaagg
    55801 ttaatcaaca agactaacgt tccatctttt gttcgccaaa ctttacggcc tgtctcattc
    55861 ttacgattgc ggcaggccgt gttattattg tcgtttctta tattttgaca ggaagagtat
    55921 cttgactact aacactcata ctctgcagat tgaagagatt ttagaacttc tgccgcaccg
    55981 tttcccgttc ttactggtgg atcgcgtgct ggattttgaa gaaggtcgtt ttctgcgcgc
    56041 agtaaaaaat gtctctgtca atgagccatt cttccagggc catttccctg gaaaaccgat
    56101 tttcccgggt gtgctgattc tggaagcaat ggcacaggca acaggtattc tggcgtttaa
    56161 aagcgtagga aaactggaac cgggtgagct gtactacttc gccggtattg acgaagcgcg
    56221 ctttaagcgc ccggtcgtgc ctggcgatca aatgatcatg gaagtcactt tcgaaaaaac
    56281 gcgccgcggc ctgacccgtt ttaaaggggt tgctctggtc gacggtaaag tagtttgcga
    56341 agcaacgatg atgtgtgctc gtagccggga ggcctgatac gtgattgata aatccgcctt
    56401 tgtgcatcca accgccattg tggaagaggg cgcgtcgatt ggcgcgaacg ctcacattgg
    56461 tcctttttgt atcgttggac cccatgtcga aattggtgag ggtaccgtac tgaaatctca
    56521 cgttgtcgtg aatggtcata ctaaaattgg ccgcgacaat gagatttatc agttcgcctc
    56581 catcggcgaa gtcaaccagg atcttaaata tgctggcgaa ccgacccgtg tggaaatcgg
    56641 cgatcgtaac cgcattcgcg aaagcgtcac cattcatcgt ggcacagtcc agggcggtgg
    56701 attgacgaag gtgggcagcg acaacttact gatgatcaac gcgcacattg cgcacgattg
    56761 tacggtaggt aaccgctgta ttctcgccaa caacgcaacg ctggcgggtc acgtatcggt
    56821 tgacgacttc gcgatcatcg gcggcatgac cgcagtccat cagttctgca tcattggtgc
    56881 gcacgtgatg gttggcggct gctccggtgt ggcgcaggac gtccctcctt atgtcattgc
    56941 gcagggtaac cacgcaacgc cgttcggtgt caatatcgaa gggctgaagc gccgcggatt
    57001 cagccgtgag gcgattaccg ctatccgcaa tgcgtataag ctgatttatc gtagcggtaa
    57061 aacgctcgat gaagtgaaac cggaaattgc tgaactggcg gaaacatatc cggaagtgaa
    57121 agcctttacc gatttctttg cacgctcaac gcgcggtctg attcgttaat gactgaacag
    57181 cgtccattaa cgattgccct ggtcgccgga gaaacctccg gcgatatcct gggggccggt
    57241 ttaatccgcg ctctgaaaga acgtgtgccc aacgcccgct ttgttggtgt tgccgggcca
    57301 cgaatgcagg ctgaaggctg cgaagcctgg tacgaaatgg aagaactggc ggtgatgggc
    57361 attgttgaag tgctcggtcg tctgcgtcgc ttactgcata ttcgtgccga tctgacaacg
    57421 cgttttggcg aactgaagcc agatgttttt gtcggtattg atgcgcctga cttcaatatt
    57481 acccttgaag gtaaccttaa aaagcagggt atcaaaacca ttcattatgt cagtccgtcc
    57541 gtctgggcgt ggcgacagaa acgcgttttc aaaataggca gagccaccga tctggtgctc
    57601 gcatttctgc ctttcgaaaa agcgttttat gacaaatata acgtgccgtg ccgttttatc
    57661 ggtcacacca tggctgatgc catgccgtta gatccagata aaaacgccgc gcgtgatgtg
    57721 ctggggatcc ctcacgatgc ccactgtctg gcattgttgc cgggaagccg tggcgcggaa
    57781 gtggagatgc ttagcgccga tttcctgaaa acggcccagc ttttgcgcca gacgtatcct
    57841 gatctggaga tcgtggtgcc gctggtaaat gccaaacgcc gtgagcaatt tgaacgcatc
    57901 aaagctgaag tcgcgccaga cctttcggtt catatgctgg atggaatggg ccgtgaggcg
    57961 atggtcgcca gcgatgcggc gctattggcg tcgggtacgg ctgcactgga gtgcatgctg
    58021 gcgaaatgcc cgatggtggt ggggtatcgc atgaagcctt tcaccttctg gttggcgaag
    58081 cggctggtga aaaccgatta tgtctcgctg ccaaacctgc tggcgggcag agagttagtt
    58141 aaagaattat tgcaggaaga gtgtgagccg caaaaactgg ctgcggcgct gttaccgctg
    58201 ttggcgaacg ggaaaaccag ccacgcgatg cacgatacct tccgtgaact gcatcagcag
    58261 atccgctgca atgccgatga gcaggcggca caagccgttc tggagttagc acaatgatcg
    58321 aatttgttta tccgcacacg cacctggttg cgggtgtgga tgaagtcgga cgcgggccgt
    58381 tagttggcgc ggtcgtcacc gctgcggtga tcctcgaccc ggcgcgcccg attgccgggc
    58441 tgaatgattc caaaaagctg agcgaaaaac gccgtctggc gctctgcgaa gagatcaaag
    58501 agaaagcgtt gagctggagt ctgggccgag cggaacctca cgaaatcgac gaactgaata
    58561 ttttacacgc gaccatgctg gcgatgcagc gtgccgtcgc tgggctgcat attgcgccgg
    58621 aatatgtgtt gattgatggt aaccgctgcc cgaaattacc gatgcctgcg atggctgtgg
    58681 tgaaaggcga tagccgcgta ccggaaatca gtgccgcgtc tatcctggcg aaagtgacgc
    58741 gtgacgccga aatggcggcg ctggatattg ttttcccgca atatggtttt gcccaacata
    58801 aagggtatcc aaccgctttt catctggaaa aactggctga atatggcgcg actgaacatc
    58861 atcgtcgcag ctttgggcct gtcaaacgcg tactgggact tgcgtcctga ttcttgtgtc
    58921 gagattaagt aaaccggaat ctgaagatgt ctgaaccacg tttcgtacac ctgcgggtgc
    58981 acagcgacta ctcgatgatc gatggtctgg ccaaaaccgc accgctggta aaaaaggcgg
    59041 cggcgttggg tatgccagca ctggcgatca ccgatttcac caacctttgc ggtctggtga
    59101 agttctacgg agcgggacat ggcgcaggga ttaagcctat cgtcggggca gattttaatg
    59161 tccagtgcga cctgctgggt gatgagttaa cccacctgac ggtgctggcg gcgaacaata
    59221 ccggctatca gaatctgacg ttgctgatct caaaagcgta tcagcgcggg tacggtgcag
    59281 ctgggccgat catcgatcgc gactggctta tcgaattaaa cgaagggttg atccttcttt
    59341 ccggcgggcg catgggcgac gtcggacgca gtcttttgcg tggtaacagc gcgctggtag
    59401 atgagtgtgt cgcgttttat gaagaacact tcccggatcg ctattttctc gagctgatcc
    59461 gcaccggcag gccggacgaa gaaagctatc tgcacgctgc ggtggaactg gcggaagcac
    59521 gcggtttgcc cgttgtggcg accaacgacg tgcgctttat cgacagcagc gactttgacg
    59581 cacacgaaat ccgcgtcgcg atccacgacg gctttaccct cgacgatcct aaacgcccgc
    59641 gtaactattc gccgcagcaa tatatgcgta gcgaagagga gatgtgcgag ctttttgccg
    59701 acattcccga agcccttgcc aacaccgttg agatcgccaa acgttgtaac gtaaccgtgc
    59761 gtcttggtga atacttcctg ccgcagttcc cgaccgggga catgagcacc gaagattatc
    59821 tggtcaagcg tgcaaaagag gggctggaag agcgtctggc ctttttattc cctgacgagg
    59881 aagaacgtgt taagcgccgc ccggaatatg acgaacgtct ggagactgaa cttcaggtta
    59941 tcaaccagat gggcttcccg ggttacttcc tcatcgttat ggaatttatc cagtggtcga
    60001 aagataacgg cgtaccagta gggccaggcc gtggctccgg tgcgggttca ctggtggcct
    60061 acgcgctgaa aatcaccgac ctcgatccgc tggaatttga cctgctgttc gaacgtttcc
    60121 ttaacccgga acgtgtctcc atgcctgact tcgacgttga cttctgtatg gagaaacgcg
    60181 accaggttat cgagcacgta gcggacatgt acggtcgtga tgcggtatcg cagatcatca
    60241 ccttcggtac gatggcggcg aaagcggtga tccgcgacgt aggtcgcgtg ctggggcatc
    60301 cgtacggctt tgtcgatcgt atctcgaaac tgatcccgcc cgatccgggg atgacgctgg
    60361 cgaaagcgtt tgaagccgag ccgcagctgc cggaaatcta cgaagcggat gaagaagtta
    60421 aggcgctgat cgacatggcg cgcaaactgg aaggggtcac ccgtaacgcc ggtaagcacg
    60481 ccggtggggt ggttatcgcg ccgaccaaaa ttaccgattt tgcgccgctt tactgcgatg
    60541 aagagggcaa acatccggtc acccagtttg ataaaagcga cgttgaatac gccggactgg
    60601 tgaagttcga cttccttggt ttgcgtacgc tcaccatcat caactgggcg ctggagatga
    60661 tcaacaagcg gcgggcgaag aatggcgagc cgccgctgga tatcgccgcg atcccgctgg
    60721 atgataagaa aagcttcgac atgctgcaac gctcggaaac cactgcggta ttccagcttg
    60781 aatcgcgcgg catgaaggac ctgatcaagc gtctgcaacc tgactgcttc gaagatatga
    60841 tcgcactggt ggcactgttc cgccccggtc cgttgcaatc agggatggtg gataacttta
    60901 tcgaccgtaa acatggtcgc gaagagatct cctatccgga cgtgcagtgg cagcatgaaa
    60961 gcctgaaacc ggtactggag ccaacctacg gcatcatcct gtatcaggaa caggtcatgc
    61021 agattgccca ggtgctttct ggttataccc tcggtggcgc agatatgctg cgtcgtgcga
    61081 tgggtaagaa aaagccggaa gagatggcta agcagcgttc tgtatttgct gaaggtgcag
    61141 aaaagaacgg aatcaacgcc gaactggcga tgaaaatctt cgacctggtg gagaaattcg
    61201 cgggttacgg atttaacaaa tcgcactctg cggcctatgc tttggtgtca tatcaaacgt
    61261 tatggctgaa agcgcactat ccggcagagt ttatggcggc ggtaatgacc gccgatatgg
    61321 acaataccga gaaggtggtg ggcctggtgg atgagtgctg gcggatgggg ctgaaaatcc
    61381 tgccaccaga tataaactcc ggtctttacc atttccacgt caacgacgac ggcgaaatcg
    61441 tgtatggtat tggcgcgatc aaaggggtag gtgaaggtcc gattgaggcc atcatcgaag
    61501 cccgtaataa aggcggctac ttccgcgaac tgtttgatct ctgcgcccga accgacacca
    61561 aaaagttaaa ccggcgggtg ctggaaaaac tgatcatgtc cggggcgttt gaccgtcttg
    61621 ggccacaccg tgcggcgctg atgaactcgc tgggcgatgc gttaaaagcg gcagatcaac
    61681 acgcgaaagc ggaagctatc ggtcaggccg atatgttcgg cgtgctggcc gaagaaccgg
    61741 aacaaattga acaatcctac gccagctgcc aaccgtggcc ggagcaggtg gtattagatg
    61801 gggaacgtga aacgttaggt ctgtacctga cgggacaccc tatcaaccag tatctaaaag
    61861 agattgagcg ttatgtcgga ggcgtaaggc tgaaagacat gcacccgaca gaacgtggta
    61921 aagtcatcac ggctgcgggg ctcgttgttg ccgcgcgggt tatggtcacc aagcgcggca
    61981 atcgtatcgg tatctgcacg ctggatgacc gttccgggcg gctggaagtg atgttgttta
    62041 ctgacgccct ggataaatac cagcaattgc tggaaaaaga ccgcatactt atcgtcagcg
    62101 gacaggtcag ctttgatgac ttcagcggtg ggcttaaaat gaccgctcgc gaagtgatgg
    62161 atattgacga agcccgggaa aaatatgctc gcgggcttgc tatctcgctg acggacaggc
    62221 aaattgatga ccagctttta aaccgactcc gtcagtctct ggaaccccac cgctctggga
    62281 caattccagt acatctctac tatcagaggg cggatgcacg cgcgcggttg cgttttggcg
    62341 cgacgtggcg tgtctctccg agcgatcgtt tattaaacga tctccgtggc ctcattggtt
    62401 cggagcaggt ggaactggag tttgactaat acaggaatac tatgagtctg aatttccttg
    62461 attttgaaca gccgattgca gagctggaag cgaaaatcga ttctctgact gcggttagcc
    62521 gtcaggatga gaaactggat attaacatcg atgaagaagt gcatcgtctg cgtgaaaaaa
    62581 gcgtagaact gacgcgtaaa atcttcgccg atctcggtgc atggcagatt gcgcaactgg
    62641 cacgccatcc acagcgtcct tataccctgg attacgttcg cctggcattt gatgaatttg
    62701 acgaactggc tggcgaccgc gcgtatgcag acgataaagc tatcgtcggt ggtatcgccc
    62761 gtctcgatgg tcgtccggtg atgatcattg gtcatcaaaa aggtcgtgaa accaaagaaa
    62821 aaattcgccg taactttggt atgccagcgc cagaaggtta ccgtaaagca ctgcgtctga
    62881 tgcaaatggc tgaacgcttt aagatgccta tcatcacctt tatcgacacc ccgggggctt
    62941 atcctggcgt gggcgcagaa gagcgtggtc agtctgaagc cattgcacgc aacctgcgtg
    63001 aaatgtctcg cctcggcgta ccggtagttt gtacggttat cggtgaaggt ggttctggcg
    63061 gtgcgctggc gattggcgtg ggcgataaag tgaatatgct gcaatacagc acctattccg
    63121 ttatctcgcc ggaaggttgt gcgtccattc tgtggaagag cgccgacaaa gcgccgctgg
    63181 cggctgaagc gatgggtatc atcgctccgc gtctgaaaga gctgaaactg atcgactcca
    63241 tcatcccgga accgctgggc ggcgctcatc gtaacccgga agcgatggcg gcatcgttga
    63301 aagcgcaact gctggcggat ctggccgatc tcgacgtgtt aagcactgaa gatttaaaaa
    63361 atcgtcgtta tcagcgcctg atgagctacg gttacgcgta attcgcaaaa gttctgaaaa
    63421 agggtcactt cggtggccct tttttatcgc cacggtttga gcaggctatg attaaggaag
    63481 gattttccag gaggaacaca tgaacatcat tgccattatg ggaccgcatg gcgtctttta
    63541 taaagatgag cccatcaaag aactggagtc ggcgctggtg gcgcaaggct ttcagattat
    63601 ctggccacaa aacagcgttg atttgctgaa atttatcgaa cataaccctc gaatttgcgg
    63661 cgtgattttt gactgggatg agtacagtct cgatttatgt agcgatatca atcagcttaa
    63721 tgaatatctc ccgctttatg ccttcatcaa cacccactcg acgatggatg tcagcgtgca
    63781 ggatatgcgg atggcgctct ggttttttga atatgcgctg gggcaggcgg aagatatcgc
    63841 cattcgtatg cgtcagtaca ccgacgaata tcttgataac attacaccgc cgttcacgaa
    63901 agccttgttt acctacgtca aagagcggaa gtacaccttt tgtacgccgg gtcatatggg
    63961 cggcaccgca tatcaaaaaa gcccggttgg ctgtctgttt tatgattttt tcggcgggaa
    64021 tactcttaag gctgatgtct ctatttcggt taccgagctt ggttcgttgc tcgaccacac
    64081 cgggccacac ctggaagcgg aagagtacat cgcgcggact tttggcgcgg aacagagtta
    64141 tatcgttacc aacggaacat cgacatcgaa caaaattgtg ggtatgtacg ccgcgccatc
    64201 cggcagtacg ctgttaattg accgcaattg tcataaatcg ctggcgcatc tgttgatgat
    64261 gaacgatgta gtgccagtct ggctgaaacc gacgcgtaat gcgttgggga ttcttggtgg
    64321 gatcccgcgc cgtgaattta ctcgcgacag catcgaagag aaagtcgctg ctaccacgca
    64381 agcacaatgg ccggttcatg cggtgattac caactccacc tatgatggct tgctctacaa
    64441 caccgactgg atcaaacaga cgctggatgt cccgtcgatt cacttcgatt ctgcctgggt
    64501 gccgtacacc cattttcatc cgatctacca gggtaaaagt ggtatgagcg gcgagcgtgt
    64561 tgcgggaaaa gtgatcttcg aaacgcaatc gacccacaaa atgctggcgg cgttatcgca
    64621 ggcttcgctg atccacatta aaggcgagta tgacgaagag gcgtttaacg aagcctttat
    64681 gatgcatacc accacctcgc ccagttatcc cattgttgct tcggttgaga cggcagcggc
    64741 gatgctgcgt ggtaatccgg gcaaacggct gattaaccgt tcagtagaac gagcgctgca
    64801 ttttcgcaaa gaggtccagc ggctgcggga agagtctgac ggctggtttt tcgatatctg
    64861 gcaaccgccg caggtggatg aagccgaatg ctggcccgtt gcgccaggcg aacgatggca
    64921 cggctttaac gatgcggatg ccgatcatat gtttctcgat ccggttaaag tcactattct
    64981 gacaccgggg atggacgagc agggcaatat gagcgaggag gggatcccgg cggcgctggt
    65041 ggcaaaattc ctcgacgaac gtgggatcgt agtagagaaa accggcccgt ataacctgct
    65101 gtttcttttt agtattggca tcgataaaac caaggcaatg ggattattgc gtgggttgac
    65161 ggaattcaaa cgctcttacg atctcaacct gcggatcaaa aatatgctac ccgatctcta
    65221 tgctgaagat cccgatttct accgcaatat gcgtattcag gatctggcgc aagggatcca
    65281 taagctgatt cgtaaacacg atctttccgg tttgatgttg cgggcatttg ataccttgcc
    65341 ggagatgatc atgacgccac atcaggcatg gcagagacag attaaaggag aagtggaaac
    65401 cattgcgctg gaacaactgg tcggtagagt atcggcaaat atgatcctgc cttatccacc
    65461 tggcgtaccg ctgctgatgc caggagaaat gctgaccaaa gagagccgca cggtactcga
    65521 ttttctactg atgctttgtt ccgtagggca acattacccc ggttttgaaa cggatattca
    65581 cggcgcgaaa caggacgaag acggcgttta ccgcgtacga gtcctaaaaa tggcgggata
    65641 acttgccaga gcggcttccg ggcgagtaac gtgctgttaa caaataaagg agacgttatg
    65701 ctgggtttaa aacaggttca ccatattgcg attattgcga cggattatgc ggtgagcaaa
    65761 gcgttttact gcgatattct tggtttcacg ctgcaaagcg aagtctatcg cgaagcgcgc
    65821 gactcgtgga aaggggattt ggcgcttaat gggcaatatg tgattgagct tttctcattt
    65881 ccgttcccgc cggagcgccc cagccgaccg gaagcttgcg gcctgcgtca tctggctttt
    65941 agcgttgatg atatcgatgc ggcagtggcg caccttgaaa gccataacgt gaagtgtgaa
    66001 gccatccgtg tcgatccata cacgcaaaaa cgcttcacgt tctttaacga tccggacggg
    66061 ctgccgttgg aactgtatga gcagtaaggc ttgtcatcgc cgcatttgcc cggtaacgtg
    66121 ccgggcattg ctactgtaaa atcgcatcat catgacactc acgctcaata gacaacttct
    66181 ctcctcacgc cagattctgg tggcctttag cggcgggctt gactccaccg ttctgctgca
    66241 tcagttggtg cagtggcgga tggaaaatcc gggcgtcact ctgcgcgcta tccatgtgca
    66301 tcacggttta agtgctaatg ccgatgcctg ggttaggcat tgcgagaaca tctgccaaca
    66361 gtggcaggtg ccgctggtgg tcgagcgcgt gcaacttgcg caagaaggac tgggcattga
    66421 ggcccaggcg cggcaggcac gttatcaggc atttgcccgc accttgttac ccggtgaagt
    66481 gctggtcacc gcgcaacatc tcgacgatca atgtgaaacc tttctgctgg cgctaaaacg
    66541 cggtagcggc cctgccgggc tttcggctat gggggaagtt tcggagtttg ccggaacacg
    66601 gcttatccgc ccgttgctcg cccgcacgcg gggagaactg gtgcagtggg cgcgtcagta
    66661 tgatttacgc tggattgaag acgaaagtaa tcaggatgat agctacgatc gtaacttcct
    66721 gcgcctgcgc gtggtgccgt tattgcagca gcgttggccg cattttgccg aagcaacggc
    66781 ccgcagcgct gcgctttgcg ctgaacaaga gagcctgttg gatgaactgc tggcggatga
    66841 tatagcacac tgtcaaacgt cgcaggggac gctgcagatt gcgccaatgc tggcgatgag
    66901 tgatgcccgc cgcgcggcga ttatccgccg ctggctggca gggaagaatg caccgatgcc
    66961 ttcccgcgac gcgttggtga ggatctggca ggaagtggcg ctggcgcggg aagatgcttc
    67021 cccctgttta cgcctgggcg cgtttgaaat ccgccgctat cagtcgcaac tgtggtggat
    67081 taaatccgtc actgggcaaa gcgaaaccat tgtgccgtgg cagacgtggc tacaaccgct
    67141 ggaattaccg gcgggactgg gaagtgtaca gcttactgcg ggaggcgata ttcgccctcc
    67201 gcgtgcagac gaagcggtca gcgtgcgttt caaagcgcca ggattgctgc atattatcgg
    67261 gcgcaacggc ggacgtaagc tgaagaaaat ctggcaagag ctgggtgtgc cgccgtggct
    67321 acgtgacacc acgccactgc tgttttatgg cgaaacgttg attgcggcgg caggggtatt
    67381 tgtgacgcaa gaaggtgtgg ctgaaggtga gaatggcgtc agttttgtct ggcagaaaac
    67441 gcttagttaa gtgaaagccg gataagacgc ttcaaacgtc gcatccggcg aaagtcaatc
    67501 aggactcgct caccaccacc gtaccgattt ccgggtggct aaagctggta attttatcca
    67561 gacgcagctc gcgggttgta ccagcggcct cgacgaccag atactccaca tttttgcggg
    67621 agactaaatc gctggccttc gcctgcaatt tttcgccatc tttcagctca agtgtcagca
    67681 ttaaatgatg ctggcaggcg agctcaagat tatcgtaatc atcacaattg attggttgat
    67741 acgtatcatt cattgacata atcgctcacc agtaagtttg ccgcagcgta tgctgctttt
    67801 tccctgacag cctcagaaag ggcgtcgtcg gcagccattt cattcagcac tttcaaaacg
    67861 cagcccagcg cgtccggaac gtatcctaag tctccgctgg cgatttccgc gtaccgcttg
    67921 cgtattaact cacaatattt ttccacatgc cctcctgtca gcactctgac ttaaccgtgg
    67981 atgcaagtct aagcctacga agataaactc tgtttcgcaa ggtgactata ccacactcat
    68041 ttctgcaata tcagcgccgc aactgcacgt attccgttac aatggcctcc tgattcgaaa
    68101 ggagttttct tatggcgctt aaagcgacaa tttataaagc gacggttaat gtggccgatc
    68161 tcgaccgcaa ccagtttctc gatgcctctc tgacgctggc gcgccaccct tcagaaaccc
    68221 aggagcgcat gatgctgcgc ctgctggcgt ggctaaaata tgccgatgaa cgtctgcaat
    68281 ttacccgtgg tttgtgtgcc gatgatgagc cggaagcgtg gctgcgtaac gatcatttgg
    68341 gtattgattt gtggattgag ctggggctgc cggatgagcg acggattaag aaagcctgca
    68401 cccaggccgc agaagtggcg ctgtttgcct ataatagtcg ggcggcgcaa atctggtggc
    68461 agcaaaatca gagcaaatgc gcgcagtttt ccaatctttc catctggtat ctggacgatg
    68521 aacaactggc gaaagtgagc gcctttgccg atcgtaccat gacgctgcag gcaacgattc
    68581 aggatggcgt gatctggtta tcggatgata agaataatct ggaagtgaac ttaaccgcct
    68641 ggcaacaacc ttcatgattg tgatttcccg acatgttgct atccccgatg gtgagcttga
    68701 gatcaccgcc attcgtgcgc agggcgcggg cgggcagcat gttaataaga cctcaacggc
    68761 tattcatctg cgttttgaca ttcgggcgtc cagcctgcca gagtattaca aagaacgtct
    68821 gctcgccgcc agccatcact tgatcagcag tgatggcgtg attgtcatta aggcacagga
    68881 ataccgcagt caggaactga accgcgaagc ggctctggcc cggctggtgg cagtgattaa
    68941 agatttaaca acagaacaaa aagcccgacg acccacgcgc cccacccgtg catcgaaaga
    69001 gcgcaggctg gcatcgaaag cacaaaaatc aagcgtgaag gcgatgcgcg gcaaagtgcg
    69061 cagcggtcgg gaataaaaag aaggaatgga tggtgaaaaa agcgatagtg acagcgatgg
    69121 ctgtaatcag cctctttact ctgatgggtt gtaataatcg ggccgaagtc gatacgcttt
    69181 ctccggcgca ggctgccgaa ctgaaaccga tgccgcaaag ttggcgcggc gtgctgccgt
    69241 gtgctgattg cgaaggaatc gaaacctctc tgttcctcga aaaagacgga acatgggtga
    69301 tgaatgagcg ttatctcggt gctcgtgaag aaccttcctc cttcgcttcc tacggtacat
    69361 gggcgcgaac cgctgacaag ctagtattaa ccgacagcaa aggtgaaaag tcatattatc
    69421 gggcgaaagg cgatgcgctg gagatgctcg atcgtgaagg caatccgatt gaatcgcagt
    69481 tcaactatac gctagaaccg gcacaatcca gtttacctat gacgccgatg accctgcggg
    69541 gcatgtattt ttatatggct gatgcggcga ccttcactga ttgcgcgacc ggaaaacgtt
    69601 tcatggtagc gaataacgca gagctggagc gtagctacct ggctgcgcgc ggtcacagtg
    69661 aaaaaccggt gttattgtca gtagaaggtc actttacgct tgagggtaat ccggataccg
    69721 gtgcgccgac taaagtattg gcacccgata cggcgggtaa attttatccg aaccaggatt
    69781 gcagtagttt ggggcagtaa ttgaaagccg gagcattctc cggcttttat cactgagtca
    69841 gcccaacaaa gcgacccgcc ttgatataaa tccctggctt catgtgccca acatactgta
    69901 actgcgtttc tggcttaaac gcagagatat cgccagtacg catatgcatc agatcggcag
    69961 ggctaatcca gcctgattgc gccagcgtaa gcggatgccc ggctttggca aatgcatcag
    70021 tgacaaattc cgaacaaaac cacgactttt tgtctccttc gcccacactg cttaactgcg
    70081 ctttcgccag gccgctgacg cactgttgac gaaaatcctc ggagaacgga ttcagtgagc
    70141 acacctggcg agtcaccata aagggaataa attcgacaat gccgcgatag ttataaccgc
    70201 tatctttgat tttattagca aacgcggtga tatctgtggc ttgttgcggg gtaagatccg
    70261 ggactcgtaa gacgaaaagc ttatcactgt gcttcatcgc ttttttaagg gaaacgatct
    70321 ggacgccagc gcctgtcgct tctgcaacgt tattatcacc cagaaagatt gcaacgtgac
    70381 tcacagagga agtgctgaag acgcggattc caaatgaggt taccccaagg cttgaggaga
    70441 acagcaaatc gccgggtttg agatcaggtg ccgttatttc ttttattgat tgttcggtga
    70501 aagagctttg atgctggaat ttgactaccc atgtttttgc ctcgacatct accgctgtgg
    70561 cagaggagtc tggctggctg atatcaacgg tacaggccga aaggaaaaga aagcagggga
    70621 gaagcaggcg gcaatgcgcc tttggtttat ccattttata caatccatgt aaaaaagggc
    70681 cctgaaattc aggacccttt ctggcatcag cctttaatct gtttcaccag atattcgacg
    70741 atgtcaccag tcttaattaa ctgtttctcg ccgttacgac gatatttata ttcgatatca
    70801 tcgttgtcga ggttacggtc gcccagcaca atagtgtgcg gaataccgat cagttccata
    70861 tcagcaaaca tcacgcccgg acgctctttg cggtcatcca gcagcacttc gataccttgt
    70921 gcacgcagtt cgctgtacag tttctcagca agctcttgta cgcggaagga tttgtgcatg
    70981 ttcatcggca gaatcgccac ctggaaaggc gcgatagcgt caggccatac gatgccgcgt
    71041 tcgtcatagt tctgctcaat tgccgcagcc acaacgcgcg ttaccccgat accgtagcaa
    71101 cccatcgtca ggatttgatt acggccatct tcaccctgta cggaggcttt cagtgcttcg
    71161 gagtacttgg tacccagctg gaagatgtga ccaacttcga taccacgttt gatcagcagc
    71221 gtaccctgac catccgggct tggatcgcca gccaccacgt tgcggatatc agcaatttcc
    71281 ggggtagcga catcgcgatc ccagttgata ccgaagtagt gtttaccatc gatgttagca
    71341 ccagcagcga aatcactcat cgccgcaacg gtacggtcaa tcaccaccgg aattggcatg
    71401 tttaccggac ccagtgagcc agggccggct ttaaccacgg cacgaatttc ttcttcggtc
    71461 gcgaaagtca gcgggcttgc aacctgcggc agtttttctg ctttaacttc gttcagctcg
    71521 tggtcaccgc gcaccagcag cgcaaccagc gggaagctac tgccttcaac cgctttaacc
    71581 agcagggtct taaccgtttt ctcaatcggc agattgaact gttcaaccag ttccgcgatg
    71641 gtcttcgcgt tcggcgtatc aaccagcgtc atttcctggg tagcagcagc gcgcggttct
    71701 ttcggcgcga tagcttctgc cagttcaatg ttcgctgcat agtcagaggt gtcggagaag
    71761 accacatcgt cttcaccgct ttgcgccagc acctggaatt cgtgagaggc gctaccgccg
    71821 atagaaccgg tatcggcctg tacggcgcgg aaatccagcc ccatgcggct gaagattttg
    71881 ctgtaggccg catacattgc atcgtaggtt tcctgcaggg attcctgaga ggtatggaaa
    71941 gagtaagcat ctttcatcag gaattcgcgg gaacgcatga cgccgaaacg cgggcgcact
    72001 tcatcacgga acttagtctg gatctgatag aagttcagcg gcagctgttt gtaagagctc
    72061 agctcgttac gaatcaggtc ggtaatcact tcttcatgag ttgggccaag tacgaacgga
    72121 cggtcaccac ggtcagcaat acgcagcaat tccgggccat actgttccca acgaccactc
    72181 tcttgccaca gttcagatgg ctgaaccacc ggcattaaca cctcaatcgc accggcgttg
    72241 ttcatctctt cacgcacgat gttttcgact tttttcagaa cgcgcacgcc ggtcggcagc
    72301 caggtgtata acccggaggc cagcttgcgg atcatcccgg cgcgcagcat cagctgatgg
    72361 ctgatcacct cggcgtcggc aggtgtctcc ttgagagtgg agagcaggta ttggctagta
    72421 cgcatgttgt tacggttcca gttggaaggt agaacaggct caaggtgagc ctgggacaaa
    72481 aaaagtgatt tagtttacca gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac
    72541 gcggttccag cgcaaagact tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat
    72601 catgcagcca gacggcatag gttttgcccg tttcctcacc tttacgatag gccgggcgcg
    72661 ggtcctgcgc tagtacttcg cggataaaca gcgttaactg cggataacgc ttctccagcg
    72721 tcaaaagctg cttttcgacc tctgcggtaa aactcaccgc catctctgca gctggcgcac
    72781 tttgcgcata gctggcactg gcatttggaa gcgattcggc aaaggggaga tacggtttga
    72841 tatccactac cggcgtacca tcgaccagat ccagactgcc gagcttcaga atcacgctgt
    72901 ctttatggca aacaacctct ttcagctcta ccagcgacat accaattggg ttagggcgaa
    72961 aagtggagcg tgttgcgaaa acccccattc tggcattacc gccaagacga ggcggacgca
    73021 cagtcggacg ccagccgcct tccatcgttt gatgaaagac gaaaagtatc cataaatggc
    73081 tgaacgcttc caggccgcga acggcgtcgg cttggttgta gggagcaatg agatgcagtt
    73141 cgccgttggc gctttttacc agacctggtt ggcgcggaac ggcgaatttt tctttatagg
    73201 gcgagcgaat aacgcctatt tgctcgaact ggaaactgct catttcgccg taatgttaag
    73261 cgcagaaccg atacatacag cctgacgata gcagcctggc gtaccgctgg tgacttcgca
    73321 gctatgcagt aataccgcat tggctttcat tttcgaggcg ttgatttgca tccgcttacg
    73381 cgcggttgga atgctcggcg gagagtcctg attagaggct tggcaagagt cgccactgac
    73441 ttcaccgaga tcgcggaacg gtttgccgac taattcttct gcattggtat aaattcggac
    73501 cggcgtggcg cgcggcactt tcggttttgc agactccgct ttcggctggg gtgcagtgct
    73561 ttgaacgggt tcgacagggg atctgcttaa catggaacag ccgcttagca tgagtgctac
    73621 taaacagatc ggtaaagcac gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt
    73681 gaatcaggag cttgtaaaaa tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa
    73741 attgtggaaa cagcctaaaa attaccagcc tttaacagcg ccgccgttaa acactttgtt
    73801 tgctgcttcg taaacttcgt cagactgata agcctggacg aatttcttca cgttctcggc
    73861 gtctttgtta tcttcacgcg ttacgatcag gtttacgtac ggggactctt tatcttcgac
    73921 aaagataccg tctttcgccg gagtcaggcc aatctggctg gcataggtgg tattgataac
    73981 tgccagagcg atttgcgcgt cgtccagaga acgcggcagc tgcggtgctt ccagctcaac
    74041 aattttcaga tttttggggt tctcaacaac atcaagaacg gtcggcagca ggccaacgcc
    74101 atctttcagt ttgatcaagc ccactttttg cagcagcagc agtgaacgac caaggttagt
    74161 tgggtcgttt ggcacggcaa cctgcgaacc atcctgcagt tcatccagtg atttgatttt
    74221 cttggagtaa cctgcaatcg gataaacaaa agtgttgcct actgcgacca gtttgtagcc
    74281 acgatctttc agttgctgat caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc
    74341 gcctttgctc aatgcttcgt ttggcagaac atagtcgttg aatgttacca gctcaacgtc
    74401 caggccatat ttgtctttcg caactttctg cgcgacttct gcaacctgct gttcggcacc
    74461 aacaatcacg ccgactttaa tgtggtttgg atctttttca tcctgaccgc agcctaccag
    74521 tgccagtgat ccaatcaggg ctcccactgc cgcaaaggtt ttgaatttga acgccatacc
    74581 ttattccttc ttcaattatt tatgttgtgt tgaacgttac ttgcgagtga cagcccggac
    74641 gatgcggtcg cctgcgaact gaattaaata aaccagaatg accagcaata ccagtaccgt
    74701 attcatcacc gtcgcgttgt agccgatgta gccatactga tagccaatct gacctaaacc
    74761 accggcaccg actgcaccac ccatcgcgga ataaccgacc agggtaatca gggtgatagt
    74821 tgccgcattt accagacccg gcaacgcttc cggtaacagc actttacgga cgatctgcat
    74881 tggcgtggca cccattgcgc gggaagcttc aattaacccg gttgggatct ccagcagagc
    74941 gttctcgacc atacgggcaa taaacggtgc tgcaccaacg gttaacggaa caatcgctgc
    75001 ctgcaaacca atcgatgtac caacaataac gcgggtaaac ggaatcatcc ataccagcaa
    75061 gataatgaac gggatggaac ggaaaatgtt cacaatcgca gaaacggtac gatacagctt
    75121 cgcgttagca ataatttgcc ccggacgcgt gacataaagc aggacgccaa ccggcagacc
    75181 aatcacaaat ccaaaaaaac cggatacgaa ggtcattgcc agcgtttccc atacgccacg
    75241 aaccagcagc cacatcatcg gctcagacat aacccagtac ctctactttt acatggtgtt
    75301 cctgcagcca ggtaatggcg gcttgcgtat cttgttgtgt gccgtgcatt tcagtcagca
    75361 tgatgccgaa cttaacgcca ccggcgtaat ccatctgcgc gctaataatg ttgttgttga
    75421 cgttgaaacg acgcgcggtt tcagaaagca gtggggcatc gaccgattga ccggtaaact
    75481 ccagacgcaa catcggcacg cagtcagtga atggctccgc ttgcagacgt tcctggtaat
    75541 cttccgggat atccagatgc agggtcgact gaataaactt ctgcgccagc ggcgttttcg
    75601 gatgcgagaa cacttcactt accgtgtcct gctcgatcag ttcgccattg ctgatgaccg
    75661 ccacgcaatc acaaatacgc ttcacaacgt ccatttcgtg ggtgatcaac agaatcgtca
    75721 aacccagacg gcggttgatg tctttcagca gttcgagaat agaacgtgtc gttgccggat
    75781 ccagcgcgct ggtggcctca tcacacagca atactttggg attgctggct aacgcacggg
    75841 caatcgccac acgctgtttc tgcccaccgg aaagattcga cgggtagcta tcatgcttat
    75901 cgccaagacc aaccaatgac agcaattccg tcacgcgacg tttgatctca tctttcggtg
    75961 tgttgtccag ctccagcggc agagccacgt tgccaaaaac agtacgcgaa gagagcaggt
    76021 taaaatgctg gaaaatcata ccaatctggc ggcgagcttt ggtcaactcg gattctgaca
    76081 gcgtggtcag ttcctggcca tcgaccagca cgctaccctc ggttgggcgc tccagcaggt
    76141 ttacacaacg tataagcgta ctcttacccg cgccagaggc accgataacg ccataaattt
    76201 gtccagctgg cacatgcagg ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt
    76261 ggaacacttt ggtgatattc gaaagtttta tcattgatta tttattatcg tcattaagtt
    76321 agtcgtggca tttcgaatgc ctggaacggg caacgccttc aatgaaatgg atgttaaggc
    76381 atccagacgt ctaaatcaat caggtttatg cgaagagcac tttcttgctg gtcgaaacat
    76441 gcgatactag cgtcacatgc cttattaagg agctataaaa ggtggcgaag agcgtacccg
    76501 caatttttct tgaccgtgat ggcaccatta atgtcgatca cggctatgtc catgagatcg
    76561 acaactttga atttatcgac ggtgttattg acgccatgcg cgagctaaaa aaaatgggct
    76621 ttgcgctggt ggtagtaacc aaccagtctg gcattgctcg cggtaaattt actgaagcac
    76681 agtttgaaac gctgaccgag tggatggact ggtcgctggc ggaccgcgat gtcgatctgg
    76741 atggtatcta ttattgcccg catcatccgc agggtagtgt tgaagagttt cgccaggtct
    76801 gcgactgccg caaaccacat ccggggatgc ttttgtcagc acgcgattat ttgcatattg
    76861 atatggccgc ttcttatatg gtgggcgata aattagaaga tatgcaggca gcagctgcgg
    76921 cgaacgtggg aacaaaagtg ctggtgcgta cgggtaaacc tgttacgcct gaagcagaaa
    76981 acgcggcgga ttgggtgtta aatagcctgg cagacctgcc gcaagcgata aaaaagcagc
    77041 aaaaaccggc gtaatgatta aaagatgagc ggttgaaata aaaatgcatt tttccgcttg
    77101 tcttcctgag ccgactccct ataatgcgcc tccatcgaca cggcggatgt gaatcacttc
    77161 acacaaacag ccggttcggt taaagagaaa aatcctgaaa ttcagggttg actctgaaag
    77221 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagccgcgtc gca
//
LOCUS       DAZKGF010000004        73853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZKGF010000004 DAZKGF010000000
VERSION     DAZKGF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.004"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(256..1629)
                     /gene="argH"
                     /locus_tag="ACHOQV_000248"
     CDS             complement(256..1629)
                     /gene="argH"
                     /locus_tag="ACHOQV_000248"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFP9628680.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVESKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1690..2466)
                     /gene="argB"
                     /locus_tag="ACHOQV_000249"
     CDS             complement(1690..2466)
                     /gene="argB"
                     /locus_tag="ACHOQV_000249"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFP9628681.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(2474..3478)
                     /gene="argC"
                     /locus_tag="ACHOQV_000250"
     CDS             complement(2474..3478)
                     /gene="argC"
                     /locus_tag="ACHOQV_000250"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFP9628682.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3632..4783
                     /gene="argE"
                     /locus_tag="ACHOQV_000251"
     CDS             3632..4783
                     /gene="argE"
                     /locus_tag="ACHOQV_000251"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFP9628683.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            5085..7736
                     /gene="ppc"
                     /locus_tag="ACHOQV_000252"
     CDS             5085..7736
                     /gene="ppc"
                     /locus_tag="ACHOQV_000252"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFP9628684.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDIYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7919..9652
                     /gene="eptC"
                     /locus_tag="ACHOQV_000253"
     CDS             7919..9652
                     /gene="eptC"
                     /locus_tag="ACHOQV_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP9628685.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9878..10729
                     /gene="yijO"
                     /locus_tag="ACHOQV_000254"
     CDS             9878..10729
                     /gene="yijO"
                     /locus_tag="ACHOQV_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9628686.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGATGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10716..11057)
                     /gene="frwD"
                     /locus_tag="ACHOQV_000255"
     CDS             complement(10716..11057)
                     /gene="frwD"
                     /locus_tag="ACHOQV_000255"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP9628687.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFDHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(11059..11937)
                     /gene="pflC"
                     /locus_tag="ACHOQV_000256"
     CDS             complement(11059..11937)
                     /gene="pflC"
                     /locus_tag="ACHOQV_000256"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFP9628688.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSQENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(11903..14200)
                     /gene="pflD"
                     /locus_tag="ACHOQV_000257"
     CDS             complement(11903..14200)
                     /gene="pflD"
                     /locus_tag="ACHOQV_000257"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9628689.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGS
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRSGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14251..14571)
                     /gene="frwB"
                     /locus_tag="ACHOQV_000258"
     CDS             complement(14251..14571)
                     /gene="frwB"
                     /locus_tag="ACHOQV_000258"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP9628690.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14586..15665)
                     /gene="frwC"
                     /locus_tag="ACHOQV_000259"
     CDS             complement(14586..15665)
                     /gene="frwC"
                     /locus_tag="ACHOQV_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFP9628691.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15974..18475
                     /gene="ptsP"
                     /locus_tag="ACHOQV_000260"
     CDS             15974..18475
                     /gene="ptsP"
                     /locus_tag="ACHOQV_000260"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9628692.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVMKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIPLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLAAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18487..19149
                     /gene="fsa"
                     /locus_tag="ACHOQV_000261"
     CDS             18487..19149
                     /gene="fsa"
                     /locus_tag="ACHOQV_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9628693.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            19160..20263
                     /gene="gldA"
                     /locus_tag="ACHOQV_000262"
     CDS             19160..20263
                     /gene="gldA"
                     /locus_tag="ACHOQV_000262"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFP9628694.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILSIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLMLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            20539..21156
                     /gene="yijF"
                     /locus_tag="ACHOQV_000263"
     CDS             20539..21156
                     /gene="yijF"
                     /locus_tag="ACHOQV_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFP9628695.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(21183..22088)
                     /gene="yijE"
                     /locus_tag="ACHOQV_000264"
     CDS             complement(21183..22088)
                     /gene="yijE"
                     /locus_tag="ACHOQV_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFP9628696.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(22182..24362)
                     /gene="katG"
                     /locus_tag="ACHOQV_000265"
     CDS             complement(22182..24362)
                     /gene="katG"
                     /locus_tag="ACHOQV_000265"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFP9628697.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24691..25581)
                     /gene="metF"
                     /locus_tag="ACHOQV_000266"
     CDS             complement(24691..25581)
                     /gene="metF"
                     /locus_tag="ACHOQV_000266"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFP9628698.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25963..27516)
                     /locus_tag="ACHOQV_000267"
     CDS             complement(25963..27516)
                     /locus_tag="ACHOQV_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9628699.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDEFKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            27649..28773
                     /locus_tag="ACHOQV_000268"
     CDS             27649..28773
                     /locus_tag="ACHOQV_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFP9628700.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTWASEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHRDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGACLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVTQMLTSLALKYNESLTPSC"
     gene            29006..30556
                     /locus_tag="ACHOQV_000269"
     CDS             29006..30556
                     /locus_tag="ACHOQV_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9628701.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKIIA
                     VDKNRLIGGMANISGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTNI
                     GKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSAKLNKITQEVVGHSP
                     VVLTREYGSSSSTGNLILDAMMEKTPDAVAGFQNSGGIRADFPKGDITLGDVISTFPF
                     NNDLIEMDLTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKP
                     VEDNQTYRIATHSFCASGGDGFEAFLNGKNVKTIPGTTSAESIIDYFKNHKPVTPDLT
                     KRVMDVAK"
     gene            30597..32216
                     /locus_tag="ACHOQV_000270"
     CDS             30597..32216
                     /locus_tag="ACHOQV_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9628702.1"
                     /translation="MKTAQRTLFAATLLTIFSTSVMAQDVTIIYTNDLHAHVDSYKVP
                     YVADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLANVYHKESEKPFWNKPYTILEKDGI
                     KIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSATLKNQGIEARDEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTPKKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSDVVIDYLKKGHKITPAQVNEMRVDVSK"
     gene            32295..33155
                     /locus_tag="ACHOQV_000271"
     CDS             32295..33155
                     /locus_tag="ACHOQV_000271"
                     /inference="COORDINATES: protein motif:HMM:NF011686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP9628703.1"
                     /translation="MCIKKHGIALSILLIPCIGNAQEIKIDESWLHQSLNVIGRTDSR
                     FGPRLTNDLYPEYTVAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPR
                     FSIDKLTGLNLAFGPFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSA
                     NIYAKYQWQNYGAANENEWDGYRFKIKYSIPLTHLFGGRLVYNSFTNFDFGSDLADKS
                     HNNKRTSNAIASSHILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGW
                     GTYTTIGYQF"
     gene            complement(33372..35804)
                     /gene="metL"
                     /locus_tag="ACHOQV_000272"
     CDS             complement(33372..35804)
                     /gene="metL"
                     /locus_tag="ACHOQV_000272"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFP9628704.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(35807..36967)
                     /gene="metB"
                     /locus_tag="ACHOQV_000273"
     CDS             complement(35807..36967)
                     /gene="metB"
                     /locus_tag="ACHOQV_000273"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP9628705.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRVALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37244..37561
                     /gene="metJ"
                     /locus_tag="ACHOQV_000274"
     CDS             37244..37561
                     /gene="metJ"
                     /locus_tag="ACHOQV_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFP9628706.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            37621..38229
                     /gene="yiiX"
                     /locus_tag="ACHOQV_000275"
     CDS             37621..38229
                     /gene="yiiX"
                     /locus_tag="ACHOQV_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFP9628707.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGELSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGKQQKLKEFDLSNPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(38290..38502)
                     /gene="rpmE"
                     /locus_tag="ACHOQV_000276"
     CDS             complement(38290..38502)
                     /gene="rpmE"
                     /locus_tag="ACHOQV_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFP9628708.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            38705..40903
                     /gene="priA"
                     /locus_tag="ACHOQV_000277"
     CDS             38705..40903
                     /gene="priA"
                     /locus_tag="ACHOQV_000277"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP9628709.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSNVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            41059..42084
                     /gene="cytR"
                     /locus_tag="ACHOQV_000278"
     CDS             41059..42084
                     /gene="cytR"
                     /locus_tag="ACHOQV_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFP9628710.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHNRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            42176..43135
                     /gene="ftsN"
                     /locus_tag="ACHOQV_000279"
     CDS             42176..43135
                     /gene="ftsN"
                     /locus_tag="ACHOQV_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFP9628711.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKIAGHTNCIRLAAGG"
     gene            43228..43758
                     /gene="hslV"
                     /locus_tag="ACHOQV_000280"
     CDS             43228..43758
                     /gene="hslV"
                     /locus_tag="ACHOQV_000280"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFP9628712.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            43768..45099
                     /gene="hslU"
                     /locus_tag="ACHOQV_000281"
     CDS             43768..45099
                     /gene="hslU"
                     /locus_tag="ACHOQV_000281"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFP9628713.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            45166..46092
                     /gene="menA"
                     /locus_tag="ACHOQV_000282"
     CDS             45166..46092
                     /gene="menA"
                     /locus_tag="ACHOQV_000282"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP9628714.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            46185..46670
                     /gene="rraA"
                     /locus_tag="ACHOQV_000283"
     CDS             46185..46670
                     /gene="rraA"
                     /locus_tag="ACHOQV_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFP9628715.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(46755..47000)
                     /gene="zapB"
                     /locus_tag="ACHOQV_000284"
     CDS             complement(46755..47000)
                     /gene="zapB"
                     /locus_tag="ACHOQV_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFP9628716.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            47426..48271
                     /gene="glpF"
                     /locus_tag="ACHOQV_000285"
     CDS             47426..48271
                     /gene="glpF"
                     /locus_tag="ACHOQV_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFP9628717.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            48294..49802
                     /gene="glpK"
                     /locus_tag="ACHOQV_000286"
     CDS             48294..49802
                     /gene="glpK"
                     /locus_tag="ACHOQV_000286"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP9628718.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            49973..50983
                     /gene="glpX"
                     /locus_tag="ACHOQV_000287"
     CDS             49973..50983
                     /gene="glpX"
                     /locus_tag="ACHOQV_000287"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP9628719.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            51080..51826
                     /gene="fpr"
                     /locus_tag="ACHOQV_000288"
     CDS             51080..51826
                     /gene="fpr"
                     /locus_tag="ACHOQV_000288"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFP9628720.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRLPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(51831..52259)
                     /gene="uspD"
                     /locus_tag="ACHOQV_000289"
     CDS             complement(51831..52259)
                     /gene="uspD"
                     /locus_tag="ACHOQV_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFP9628721.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(52286..52585)
                     /gene="yiiS"
                     /locus_tag="ACHOQV_000290"
     CDS             complement(52286..52585)
                     /gene="yiiS"
                     /locus_tag="ACHOQV_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFP9628722.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(52797..53237)
                     /gene="yiiR"
                     /locus_tag="ACHOQV_000291"
     CDS             complement(52797..53237)
                     /gene="yiiR"
                     /locus_tag="ACHOQV_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9628723.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            53338..53937
                     /gene="yiiQ"
                     /locus_tag="ACHOQV_000292"
     CDS             53338..53937
                     /gene="yiiQ"
                     /locus_tag="ACHOQV_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFP9628724.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQALLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            54045..54812
                     /gene="tpiA"
                     /locus_tag="ACHOQV_000293"
     CDS             54045..54812
                     /gene="tpiA"
                     /locus_tag="ACHOQV_000293"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP9628725.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(54867..55622)
                     /gene="cdh"
                     /locus_tag="ACHOQV_000294"
     CDS             complement(54867..55622)
                     /gene="cdh"
                     /locus_tag="ACHOQV_000294"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFP9628726.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(55728..56717)
                     /gene="sbp"
                     /locus_tag="ACHOQV_000295"
     CDS             complement(55728..56717)
                     /gene="sbp"
                     /locus_tag="ACHOQV_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFP9628727.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKVFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(57037..57999)
                     /gene="pfkA"
                     /locus_tag="ACHOQV_000296"
     CDS             complement(57037..57999)
                     /gene="pfkA"
                     /locus_tag="ACHOQV_000296"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP9628728.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIELL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(58180..59082)
                     /gene="fieF"
                     /locus_tag="ACHOQV_000297"
     CDS             complement(58180..59082)
                     /gene="fieF"
                     /locus_tag="ACHOQV_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFP9628729.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(59231..59731)
                     /gene="cpxP"
                     /locus_tag="ACHOQV_000298"
     CDS             complement(59231..59731)
                     /gene="cpxP"
                     /locus_tag="ACHOQV_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFP9628730.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            59881..60579
                     /gene="cpxR"
                     /locus_tag="ACHOQV_000299"
     CDS             59881..60579
                     /gene="cpxR"
                     /locus_tag="ACHOQV_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFP9628731.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            60576..61949
                     /gene="cpxA"
                     /locus_tag="ACHOQV_000300"
     CDS             60576..61949
                     /gene="cpxA"
                     /locus_tag="ACHOQV_000300"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFP9628732.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(62055..62729)
                     /gene="yiiM"
                     /locus_tag="ACHOQV_000301"
     CDS             complement(62055..62729)
                     /gene="yiiM"
                     /locus_tag="ACHOQV_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFP9628733.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGVSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(62878..63861)
                     /gene="kdgT"
                     /locus_tag="ACHOQV_000302"
     CDS             complement(62878..63861)
                     /gene="kdgT"
                     /locus_tag="ACHOQV_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFP9628734.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPEAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(64121..64741)
                     /gene="sodA"
                     /locus_tag="ACHOQV_000303"
     CDS             complement(64121..64741)
                     /gene="sodA"
                     /locus_tag="ACHOQV_000303"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFP9628735.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            65026..66060
                     /gene="rhaT"
                     /locus_tag="ACHOQV_000304"
     CDS             65026..66060
                     /gene="rhaT"
                     /locus_tag="ACHOQV_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFP9628736.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            complement(66057..66905)
                     /gene="rhaR"
                     /locus_tag="ACHOQV_000305"
     CDS             complement(66057..66905)
                     /gene="rhaR"
                     /locus_tag="ACHOQV_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFP9628737.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQIIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(66979..67815)
                     /gene="rhaS"
                     /locus_tag="ACHOQV_000306"
     CDS             complement(66979..67815)
                     /gene="rhaS"
                     /locus_tag="ACHOQV_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFP9628738.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEEENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARYLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            68103..69572
                     /gene="rhaB"
                     /locus_tag="ACHOQV_000307"
     CDS             68103..69572
                     /gene="rhaB"
                     /locus_tag="ACHOQV_000307"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP9628739.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTHGLMAQAQRQLGKCDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQSNEIPVVAVASHDTASAVIASPLNSSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFTINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            69569..70828
                     /gene="rhaA"
                     /locus_tag="ACHOQV_000308"
     CDS             69569..70828
                     /gene="rhaA"
                     /locus_tag="ACHOQV_000308"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP9628740.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVARDQIKPEHFKNWVEWAKTNQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLESAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRAYEKEILSRRG"
     gene            71013..71837
                     /gene="rhaD"
                     /locus_tag="ACHOQV_000309"
     CDS             71013..71837
                     /gene="rhaD"
                     /locus_tag="ACHOQV_000309"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP9628741.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNKAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            71847..72161
                     /gene="rhaM"
                     /locus_tag="ACHOQV_000310"
     CDS             71847..72161
                     /gene="rhaM"
                     /locus_tag="ACHOQV_000310"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP9628742.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPTNPDNSPVSSELQEVFY
                     LP"
     gene            complement(72202..73596)
                     /locus_tag="ACHOQV_000311"
     CDS             complement(72202..73596)
                     /locus_tag="ACHOQV_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFP9628743.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATSESAKKNSTSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGVVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT        18364 a        19450 c        18569 g        17470 t
ORIGIN      
        1 tatattcaat taattaaagt atgtattggc tgatgtattg aaaatataat ttttatgggg
       61 agagtgaagg taaagaagac gcaagaataa aaaggaatgt gacaaataat ggttctggtt
      121 gggcgttaaa tttaaggtca ccagaatact gacaaatttc tgcctgatgc ggcgtaaacg
      181 ccttatccgc ctacgaagtg tcgcactttg taggcgtggt aagctcccca ttttcagggg
      241 agcaatataa acaaactact ctaaccgagc ttgcgcaaaa gcaatcgcct gcgccacctg
      301 ctgcggtgag acgccgcctt ttgcagcacg cttgtcgagg cacgattgca gcgacagaat
      361 cggatagaca tcttcgccaa tcacctggct gaatttctgc aactcactga gcggcagatc
      421 ttccaacgct ttgccctgac gaatggcttc caccacagct tcacccacaa tatggtgcgc
      481 ctcgcggaac ggtacgcctt tcgccaccag ataatccgcc agttcagtag cgttggcgta
      541 accctgctgc gccgcttcct ggcaacgcgg acgtttcacc tgaatgccgt ccagcaccag
      601 cgccgccata tgcaggcagt ccagccaggt atcgagcgcg tcgaacagac cttctttgtc
      661 ttcctgcata tctttgttgt aagccagcgg caaacctttc agcgtcatca tcatgccggt
      721 taacgccccc tgcacccgac cgcatttacc gcgaatcagc tccagcgcat ccgggttttt
      781 cttctgcggc attaatgatg aaccggaagt cacacggtca gaaagctcca caaaccccgc
      841 ttcgccggtg ttaaagaaaa tcagatcttc agcaaaacgc gacagatgca ccatgccgat
      901 agcggcagca gaaagcagtt ccagcacatg gtcacggtca gaaacgctgt cgagactgtt
      961 acgggtcgcc gaagcaaagc ccagccagcc tgctaactgt tcacggtcga tttcataggc
     1021 cgttcctgcc agtgcgccac agcctagcgg gctgacatcc agacgcttaa gcgcgtcctg
     1081 caaacggctt tcatcacgcg ccagcatctc aacgtaggcc aggcaccagt gagcgaacgt
     1141 caccggctgg gcgcgttgca ggtgagtgta acccggcatc accgcgtcct gattgttctg
     1201 cgcggtctcg accagagcgg attgtaactg ccggttagcc gtcagcaact cgctaacggt
     1261 atctttgcac cacagtttca ggtcagttgc tacctgatca ttacggctac gcccggtatg
     1321 cagttttttg cctaactgac ccactttgtc gatcagtttg ctttccaccc agctatggat
     1381 atcttcggcg tcgctttcaa ggatttgttg tggcctggcg cgaacatctt ccagcagcac
     1441 gttcagcgcc tcttccagtt gcgcctgctc ttctgcggtt aacacgccga ccgttaccag
     1501 ggctttggac caggccacag agccaacaat atcctgctcc gccagacggt aatcaaagcg
     1561 cagtgagtcg ttgaattgtt tgaaccgttg atctgctgcc tgggtaaaac gcccgcccca
     1621 aagtgccata actctgtttc cttattttga aattcaatgc cggaaagctg cgcctccggc
     1681 caacaaaact taagctaaaa tccgcgtacc catcggcata ccgttaaaca gtgccggaag
     1741 ctgttccgca tgacgccagg aggcgatatc taccggacgg cccaacgtgc gcgcagcatc
     1801 cagcgccgcg ttcactttca ctatcatgcc gtcagtaata ataccctgct caatcagttg
     1861 ttctgctttc gcggcggtca tttcggcaat acgttgccct ttgccgtcga gaatgccact
     1921 gacgtcggag agcaaaatca gatccgcgcc cagcgttgcc gccagcgccg ttgccgcctg
     1981 atcggcattg acgttcatca gttgcccttc gtctgttacg ccaatggagc tgaccaccgg
     2041 cagataaccg ttctccagca atgagttgat aagcttaggc gaacctggct gcgccagtcc
     2101 aacatggcct aactcttcat caagctgggt cactttgacg ctgtcgccgt caccgagaaa
     2161 caaacctacg gccgcaatct gatgtttctt cgcccatgcc agcagggttt tatttgccgt
     2221 tcccgccagt gctccggtga taatgtcaat ctgatcagca ggcgtcaccc gcaggccatt
     2281 tttctttttc accggcagat tcagcccttt catcaactca tccactacgc agccgccacc
     2341 gtggacaatc accagcggac gctgatgtga ctcacgataa ttcaccagtg cactaaacag
     2401 acgttccagc gcctcctcac tatccagcag tacgccgccc agtttgataa ttaatggatt
     2461 catcattgca cccttaaata agagactgcg tttccgcata gccgaaacga atattggcgc
     2521 actgcaccgc ttgtgccgcc gcgcctttca gtaagttgtc ttcggtcgcc acaataatca
     2581 gatgctcgcc ctgaacggca aaaccgatat cgcagaatgg cagtccaacg acatttttca
     2641 gcgccggaac gcctttgtca tacagccgca ccagtggttt atgggcatac gcctgctgta
     2701 acgcttgcgc cacttgcacc tggctcacac ccggtttcag gcggcaggta atggtttcga
     2761 gaatgccgcg cgggaaattg cccagatgcg gggtgaagat aacgtcagca ccgaggtgtg
     2821 tggcgatctc tggttgatgg cgatgggtaa agacgccata cggttgcagg ctaacttcac
     2881 aaaaactatt tgaaatcgct gccttacgtc ctgcaccgct cacgccgctg gtggcgttga
     2941 tcaccggcca ctgattgagg tcaagaagat cggcatcaat caacggtttc agcgccagct
     3001 gtgctgccgt cggataacag cctggcacgg caatcaagtt cgcttctttt aatttattac
     3061 cgcaccactc cgccagaccg taggctgcct gttccagcag ttccgggtat tgatgggtaa
     3121 agccgtaata tttttcatag aaggcgacat cgttaacacg aaacgcgccg gaaaggtcga
     3181 acaccacaca gcctgcttca agaaattgcg gcgctaaatc gtggctgact tcatgggcgg
     3241 tggcgagaaa caccacgtcc acccctgggc taaactcgct gatatccgac atcggctgca
     3301 acggcagatc aacgatgcct tttagctgcg gatgcaaatc ggagattaac tttcccgcat
     3361 cattgctttg cgctgaaaca gtcaaagcgg ttatgttcat atgcggatgg cgatttacat
     3421 aggtcactag ctctgcgcca gcgtagccgc tggcacccac aatcagcgta ttcaacatcg
     3481 gggctattca ccttcttatg tctggttgcc aggttaaacg taaaacattc accttacggc
     3541 tggtgggttt tattacgctc aacgttagtg tatttttatt cataaatact gcatgaatat
     3601 tgatactatc atgaccagag gtgtgtcaac aatgaaaaac aaattaccgc catttatcga
     3661 gatttaccgt gctctgattg ccacaccttc aataagcgcc acggaagagg cactcgatca
     3721 aagcaatgca gatttaatca ctctgctggc ggactggttt aaggatttag gcttcaatgt
     3781 ggaagtacag cctgtgccag gaactcgcaa caaatttaat atgctggcca gttgcggaca
     3841 gggggctggc ggtttgttgc tggcggggca taccgatacg gtgccatttg atgacggacg
     3901 ctggacgcgc gatccattta cgttgacgga gcatgacggc aagctttacg gcttaggcac
     3961 cgccgacatg aaaggctttt ttgcgtttat ccttgatgcg ctacgcgatg tcgacgtcac
     4021 gaagcttgca aaaccgctct acattctggc gactgccgat gaagaaacca gtatggccgg
     4081 agcgcgttat tttgccgaaa ctaccgccct gcgcccggat tgcgccatca ttggcgagcc
     4141 aacgtcgctg caaccggtac gcgcgcataa aggtcatatc tctaacgcca tccgtattca
     4201 gggccagtcg ggccactcca gcgatccagc acgcggagtt aacgctatcg aactgatgca
     4261 cgacgccatc gggcatattt tgcaattgcg cgataacctg aaagaacgtt atcactacga
     4321 cgcgtttacc gtgccatacc ctacgctcaa cctcgggcat atccacggtg gcgacgcttc
     4381 taaccgtatt tgtgcttgct gtgagttgca tatggatatt cgtccgctgc caggcatgac
     4441 actcaatgaa cttaatggtt tgctcaacga tgcattggct ccagtgagcg aacgctggcc
     4501 gggtcgtctg acggtcgacg agctgcatcc gccgatccct ggctatgaat gcccaccgaa
     4561 tcataaactg gttgaagtgg ttgagaaatt gctcggagca aagaccgaag tggtgaatta
     4621 ctgtaccgaa gcgccgttta ttcaaacgtt atgcccgacg ctggttctgg ggcctggctc
     4681 aattaatcag gctcatcagc ctgatgaata tctggagacg aggtttatca agcccacccg
     4741 cgaactgatc acccaggtga ttcaccattt ttgctggcat taaaacgtag gccaaacaat
     4801 gtcggatgcg atgcttacgc atcttatccg acctacacct ttggtgttac tcgaggcgat
     4861 tttttaacat tcccataagt tacgcttatt taaagcgtca tgaatttaat ggcgtaaatt
     4921 cctgctattt attcgtttgc tgaagcgatt tcgcagcatt tgacgtcacc gcttttacgt
     4981 ggctttataa aagacgacga aaagcaaagc ccgagcatat tcgcgccaat gcgacgtgaa
     5041 ggatacaggg ctatcaaacg ataagatggg gtgtctgggg taatatgaac gaacaatatt
     5101 ccgcattgcg tagtaatgtc agtatgctcg gcaaagtgct gggagaaacc atcaaggatg
     5161 cgttgggaga acacattctt gaacgcgtag aaactatccg taagttgtcg aaatcttcac
     5221 gcgctggcaa tgatgctaac cgccaggagt tgctcaccac cttacaaaat ttgtcgaacg
     5281 acgagctgct gcccgttgcg cgtgcgttta gtcagttcct gaacctggcc aacaccgctg
     5341 agcaatacca cagcatttcg ccgaaaggcg aagctgccag caacccggaa gtgatcgccc
     5401 gcaccctgcg taaactgaaa aaccagccgg aactgagcga agacaccatc aaaaaagcag
     5461 tggaatcgct gtcgctggaa ctggtcctca cggctcaccc aaccgaaatt acccgtcgta
     5521 cactgatcca caaaatggtg gaagtgaacg cctgcttaaa acagctcgat aacaaagata
     5581 tcgccgacta cgaacataac cagctgatgc gccgcctgcg ccagttgatc gcccagtcat
     5641 ggcatacaga tgaaatccgt aagctgcgtc caagcccggt agatgaagcc aaatggggct
     5701 ttgccgtagt agaaaacagc ctgtggcaag gcgtaccgaa ttacctgcgc gaactgaacg
     5761 aacaactgga agagaatctc ggctacaaac tgcccgtcga atttgttccg gtccgtttta
     5821 cctcgtggat gggcggtgac cgcgacggca acccgaacgt cactgccgat atcacccgcc
     5881 acgtcctgct gctcagtcgc tggaaagcca ccgatctgtt cctgaaagat attcaggtgc
     5941 tggtttctga actgtcgatg gttgaagcga cccctgaact gctggcgctg gttggcgaag
     6001 aaggtgccgc agaaccgtat cgctatctga tgaaaaacct gcgttctcgc ctgatggcga
     6061 cacaggcatg gctggaagcg cgcctgaaag gcgaagaact gccaaaacca gaaggcctgc
     6121 tgacacaaaa cgaagaactg tgggaaccgc tctacgcttg ctaccagtca cttcaggcgt
     6181 gtggcatggg tattatcgcc aacggcgatc tgctcgacac cctgcgccgc gtgaaatgtt
     6241 tcggcgtacc gctggtccgt attgatatcc gtcaggagag cacccgccat accgaagcgc
     6301 tgggcgagct gacccgctac ctcggtatcg gcgactacga gagctggtca gaagccgaca
     6361 aacaggcgtt cctgatccgc gaactgaact ccaaacgtcc gcttctaccg cgcaactggc
     6421 aaccaagcgc cgaaacgcgc gaagtgctcg atacctgcca ggtgattgcc gaagcaccgc
     6481 aaggctccat tgccgcctac gtgatctcga tggcgaaaac gccgtccgac gtgctggctg
     6541 tccacctgct gctgaaagaa gcgggtatcg ggtttgcgat gccggttgct ccgctgtttg
     6601 aaaccctcga tgacctgaac aacgccaacg atgtcatgac ccagctgctg aatatcgact
     6661 ggtatcgcgg cctgattcag ggcaaacaga tggtgatgat tggctattcc gactcagcaa
     6721 aagatgcggg cgtgatggca gcttcctggg cgcaatatca ggcacaggat gcattaatca
     6781 aaacctgcga aaaagcgggt attgagctaa cgttgttcca cggtcgcggc ggttccattg
     6841 gtcgcggcgg cgcacctgcc catgcggcgc tgctgtcaca accaccagga agcctgaaag
     6901 gcggcctgcg cgtgaccgaa cagggcgaga tgatccgctt caagtacggt ctgcctgaaa
     6961 tcaccgttag cagcctttcg ctgtataccg gggcgattct ggaagccaac ctgctgccac
     7021 cgccagagcc gaaagagagc tggcgtcgca ttatggatga gctttcggtt atctcatgcg
     7081 atatctaccg cggctacgtg cgtgaaaaca aagattttgt gccttacttc cgctctgcca
     7141 cgccggagca ggaactcggc aaactaccgc tgggttcacg tccggcaaaa cgtcgcccga
     7201 ccggtggcgt tgagtcactg cgcgctattc cgtggatttt tgcctggaca caaaaccgcc
     7261 tgatgctccc cgcctggctg ggtgcaggta cggcgctgca aaaagtggtc gaagatggca
     7321 aacagagcga gctggaagcc atgtgccgcg attggccatt cttctcgacg cgtctcggca
     7381 tgctggagat ggtcttcgcc aaagcagacc tgtggctggc ggaatactat gaccaacgcc
     7441 tggtagacaa agcactgtgg ccgttaggta aagagttacg caatctgcaa gaagaagaca
     7501 tcaaagtggt gctggcgatt gccaacgatt ctcacctgat ggccgatctg ccgtggattg
     7561 cagagtctat tcagctacgg aatatttaca ccgacccgct gaacgtattg caggccgagt
     7621 tgctgcaccg ctcccgccag gcagaaaaag aaggccatga gccggatcct cgcgtcgagc
     7681 aggcgttaat ggtcactatt gccgggattg cggcaggtat gcgtaatacc ggctaatctt
     7741 cctctcctgc aaaccctcgt gcttttgcgc gagggttttc tgaaatactt ctgttctaac
     7801 accctcgttt tcaatatatt tctgtctgca ttttattcaa aatctggata tcccttcaga
     7861 tatccttaag gaattgtcgt tacattcggc gatatttttt tcaagacagg ttcttactat
     7921 gcattccaca gaagtccagg ctaaacccct ttttagctgg aaggccctgg gttgggcact
     7981 gctctacttt tggtttttct ctactctgct acaggccatt atttacatca gtggttatag
     8041 tggcactaac ggcattcgcg actcgctgtt attcagttcg ctgtggttga tcccggtatt
     8101 cctctttccg aagcggatta aaattattgc cgcagtgatc ggcgtggtgc tatgggcggc
     8161 ctctctggca gcgttgtgct actacgtcat ctacggtcag gagttctcgc agagcgttct
     8221 gtttgtgatg tttgaaacca acaccaacga agccagcgag tatttaagcc agtatttcag
     8281 cctgaagatt gtgcttatcg cgctggccta tacggcggtg gcagttttgc tgtggacgcg
     8341 cctgcgcccg gtctatattc caaagccgtg gcgttatgtt gtctcttttg ccctacttta
     8401 tggcttgatt ctgcatccga tcgccatgaa tacgtttatc aaaaacaagc catttgagaa
     8461 aacgttggat aacctggcat cgcgtatgga gcctgccgca ccgtggcaat tcctgaccgg
     8521 ctattatcag tatcgtcagc aactaaactc gctaacaaag ctactgaatg aaaataatgc
     8581 cttgccgccg ctggcgaatt tcaaagatga atcgggtaac gaaccgcgca ccttagtgct
     8641 ggtaattggc gaatcgaccc agcgtggacg catgagtctg tacggttatc cgcgtgaaac
     8701 cacgccggag ctggatgcgc tgcataaaac cgatccgaat ctgaccgtgt ttaataacgt
     8761 ggttacgtct cgtccgtaca ccattgaaat cctgcaacag gcgctgacct ttgccaatga
     8821 aaagaacccg gatctgtatc tgacgcagcc gtcgctgatg aacatgatga aacaggcggg
     8881 ttataaaacc ttctggatca ccaaccagca gacgatgacc gcccgcaata ccatgctgac
     8941 ggtattttcg cgccagaccg ataagcagta ctacatgaac cagcaacgta cgcagagtgc
     9001 gcgtgaatac gacaccaacg tgctgaagcc gttccaggaa gtgctgaagg accctgcgcc
     9061 gaagaaactg atcatcgttc atctgctggg tacgcatatc aagtacaagt accgttatcc
     9121 ggaagatcag ggcaaatttg acggtaatac agagcatgtt ccaccgggat taaacgcaga
     9181 agagctggag tcatataatg attatgacaa tgctaacctg tacaacgatc acgtggttgc
     9241 cagcctgatt aaagacttta aagcgaccga tccgaacggt ttcctggttt atttctctga
     9301 ccacggtgaa gaggtttacg acacgccgcc gcacaaaact caggggcgta atgaagacaa
     9361 cccgacgcgt catatgtaca ccattccgtt cctgctgtgg acgtcagaaa aatggcaagc
     9421 gactcatccg cgtgatttct cacaggatgt tgatcgtaaa tacagcctgg cggaactgat
     9481 ccacacctgg tcagatttgg cgggcttatc ttacgacggt tacgatccaa cccgttcagt
     9541 ggtgaatccg cagttcaaag aaactacccg ctggattggt aacccgtata agaaaaacgc
     9601 actgatcgat tacgacactc tgccgtatgg cgatcaggtg ggtaatcagt aattatcagc
     9661 cagatgttat ttgccggatg cgacgcttga cgcgtcttat ccggcctaca gatcgcggcg
     9721 gtaggccgga taaggcgttt acgccgcatc cggcaataca agtcgactga aactgccgca
     9781 atgtttacat ctcactccca tcaggccaca cttttcaggt ggcctttttt cataatgcca
     9841 gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg tcagctatct
     9901 gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg ccagcagcaa
     9961 cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa ttgtgctgga
    10021 aggtgagttt attgataccg gcgctggagc agcgttagtt cccggcgatg tgctgtacgt
    10081 tcccgctggt ggctggaatt ttccacaatg gcaagccccc gccactacct ttagcgtgct
    10141 gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac aatatcaaaa
    10201 tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc tgctacaaac
    10261 gctcaatgaa atgcaaatgc agtcgcagga gcagcaaacg gcaaggctta tcgtcgccag
    10321 cctgcttagc cactgccgcg atttgcttgg cagccaaatc cagaccgcct cacgcagcca
    10381 ggcactgttc gaagctattc gtgattatat cgacgaacgc tatgcctccg cgcttacccg
    10441 cgaatctgtc gcacaggcat tctatatttc gccaaattac ctctcgcacc tgtttcaaaa
    10501 aacgggggcc actggtttta acgaatacct gaatcacacg cgactggagc acgctaagac
    10561 gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg gctttgtcga
    10621 cagcaactac ttctgccgtc tgtttcgtaa aaataccgaa cgctcaccgt cggaatatcg
    10681 tcggcagtac cacagccaac tgacagaaaa accgactact ccagaatgag atgggtttgc
    10741 tgcggcgcag aaagcacttt gcgtaccgcg ctcatcaccc gctgcggctc acgaaggaaa
    10801 gcgtagatgc tgcattgcac atagcggcaa tggtcaaatc gctcagcacc ggcaagctcg
    10861 atatccgtaa ttaacagcgc aacatccgcc cggcggatat cctcgtccgc taaacgattc
    10921 tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa caggcacaac
    10981 ttttccagcc gttccgccgc catataagta tgcgccacgc cactgacgca ggcggttact
    11041 gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc gttctgccat
    11101 ttcgcgcatc gttgccacat cagctgacga cggcgcaggc acctctttca tcgaccatgt
    11161 tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca gatggatctg
    11221 cctgatattc agcgggatca gcacgtccag agcctgctgc atattctcct ggctgagcgt
    11281 gaaaccaggg atcagcggta aacgcgggat cacgttgacg ccctcactca ccagcaaacg
    11341 cagattctcc agcacgcgtg gcagattcat cttcaccaca tcccgcgcct gagtcgcgtc
    11401 cataattttt aaatcgaaca acacttcatc gcacaatttc gccagcggta acagcttgct
    11461 ggctggcgcg tctccggcag tttcaatggc gcatgacacg ccccacaacc gcagccgctg
    11521 taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa gcgtgacgcc
    11581 gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgctcca gcgcgtcaag
    11641 gctgatatcg cggccaatcc gttcaaacgc cccggagggg cattcatccg catcacgcaa
    11701 acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt tgccggagat
    11761 cgactccgga ttagcacacc atgggcaaag atgcggacag cctttaaaaa agaccaccgt
    11821 acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc gcgccacatc
    11881 attgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg atgtcatcct
    11941 ggatctcctt cgacaattcg acaaagaagg cgctgtatcc ggcaacgcgc accaccagcc
    12001 cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc accacgttaa
    12061 actgaatatg ctgcaactta agctgggtaa acgcccgtaa gaagtcggcc agtttgcgta
    12121 atcccgcttc accttccaga gtcgccggag tgaatttcac gttcagcaac gtaccgttag
    12181 acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc ccttgtgcgt
    12241 cctggcccag catgggtgac aagccgccgt ctgccagttg ttccccggca aaacgaccgt
    12301 ctggcgtcgc gccaaccacc gatcccaacg ggacgtgagc agaaacggta tacgatcccg
    12361 gcgtgaagta gccgctgcgc gggttctggt atttttccac ttctttgcag tagtggcgta
    12421 acagttcggc gctgatgtta tccacctcgt cgatatcgtt accgtatttc tcgaagcggt
    12481 taattaagcg agcacggact ttttcgcctt ctggcgttgc gaagttggct tttaataccg
    12541 acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc aacgcatgga
    12601 gagaatcgct caggttggcg ataccgatcc cctgtacgcc ggagaagtta taacgcgcgc
    12661 cgccatcggt aatatcgctg cctttttcca gacaatcgct gataaacgat gagagcagcg
    12721 gtacaggtgc ccagtcacgg tgaccgatat cgcaaatatt gctgccctca accatcaggg
    12781 tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa gtcagcgcag
    12841 cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta aacatcgcga
    12901 tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc actacggaat
    12961 agtcgcgtgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc accacttcat
    13021 cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttctgcggtt ttcatcagga
    13081 acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt aattgcacgc
    13141 tttgataagc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt ccgttctcgg
    13201 ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa cgcgcgctac
    13261 tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg agcagttctt
    13321 tcaggaacgc cggatcttcg ccctgggtta atgatgcctg atagaacggc aacatatact
    13381 ggtcgaagcg ccccaacgat agtgaactgg cgttggattc gtattgcaga atgatgttca
    13441 tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttcg gcgttatggc
    13501 gggagatctc cgcaatagtc agcagttctt cgcgacgctg ggcatcagtg cagcttgccg
    13561 ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct tcgagcagta
    13621 acagcgctgc ctgataaaag tgattctccg gctgttgctg acaatgttgc tgcatctgcg
    13681 ctaccagctc acccagcccg tgattcagca ggcgcgggta atcaataata atgtgcccct
    13741 ggcctttatc cgtctggttg atgctgaaaa tctgcgtgct ggtcgcggct tttacttcat
    13801 ccgtcatctg cccgttaatg aaatctttca tcgaacgttt ttcccagtac gggaacaact
    13861 cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc ggacgcgtcg
    13921 ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac ataatcccgg
    13981 cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc gaaatttcaa
    14041 catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt tcgccttcgg
    14101 tttgccgatg gctggcggta taaagcagcg cacgctccag cgagatttca cgggtattgg
    14161 caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc tgtgggaaag
    14221 cgaacccgcc cttacgggcg ggataagaac ttaagcggtt tgtgccaggt gagcttcaat
    14281 tttgctcata atggcatcag cgcgttttac cgcgtcgctg atgttgacgc gcacgatggt
    14341 cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagga tcaccatatc
    14401 ggcgctcgcc acgtcttccg cagtcagttc gttttccaga ccaatcgagc cctgggtttc
    14461 tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg ccgccatata
    14521 ggtatgggca acaccagatg gacacgcggt tactgcaata attttcgtca tggctcgttc
    14581 ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc agtcgaagac
    14641 ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac cactgctccg
    14701 acagctactg ctgcgatata acccagcttg ccttcaacca ccggcagcac aatcagaccg
    14761 ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc acaaactgaa
    14821 ccgaccatga tggaaggaat aacacgcagc ggatcggcag cggcgaaagg aatcgcccct
    14881 tcagtaaccc caacgcaccc catcaccagc gccgctttac cggtttcgcg ctcttctgcg
    14941 gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg aatacaaata
    15001 cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat cagcatgaag
    15061 gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag gcccatgatc
    15121 accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt caggctgttg
    15181 gtcaatgccc ccacaggctc gcccagcccc cacatcatga tgcctgcggt aatcagtgtg
    15241 ccgacgatcg gaatgatgaa gattggcatc accgagcgca gaactttatg caccggaatt
    15301 ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagtgc accaaagaag
    15361 cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag cgcagaacgc
    15421 tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt caaacccgca
    15481 acgccgatat caaacagttt tttcagattc ggatcggcta cggcatccgg cactgcgcct
    15541 ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac gaagggaatc
    15601 atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg caccaactca
    15661 ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg gaggaaacaa
    15721 taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga cttttgtaat
    15781 atcagtacaa aaatgcgatc cgcctcataa cttgcgatga tccggaagca tgccgcaaaa
    15841 cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc agcttttggc
    15901 agatttgtca catggcaggc cgttgccgtg cgctcttatc ctgtaaacag aaaaccgtac
    15961 cgggagagag gcaatggccc tgattgtgga atttatttgt gagctaccta acggcgtaca
    16021 tgcacgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatcac aaattgagtg
    16081 gcataacctg cgcactgacc gcaagggcaa cgccaaaagc gcccttgcgc tgattggcac
    16141 cgatacgctg gtgggcgata actgccagtt actgatttcc ggggccgacg aacaggaagc
    16201 gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgacg caccgctggc
    16261 ggaagtaaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc tgaatccgca
    16321 aattatccgt gctcgcaccg tgtgcagtgg tagtgcaggc ggcattctga cgccgatctc
    16381 ttctttagat ctcaatgcgc tgggtaatct tcctgctgcc aaaggcgttg acgccgagca
    16441 atccgcgctg gaaaacggcc tgacactggt gatgaaaaac attgaatttc gtctgctgga
    16501 tagcgacggt gctaccagcg cgatactgga agctcaccga tccctggctg gcgatacttc
    16561 cctgcgcgaa catttactgg caggtgtcag cgccggatta agctgcgccg aagcgattgt
    16621 tgccagtgcg aatcactttt gcgaagagtt cgcccgttcc agcagcagct acctgcaaga
    16681 acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct acggtgagca
    16741 acgcttcccg gcaccgggca aactgacgca gcccgccatt tgtatggctg atgaactgac
    16801 ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggtctattac tgaaaagtgg
    16861 cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga cgctggtagg
    16921 tgtggatatt gatgccctta ctccgtggca gcatcaaacg atttatatcg acggcaacgc
    16981 aggggcgatt gtggttgagc caggggaagc cgtggcccgt tattatcagc aagaagcccg
    17041 cgtacaggac gccctgcgtg agcaacagcg tgtctggctg acccaacaag cccgtaccgc
    17101 tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag cgcaggccgc
    17161 attcggcaat ggtgcggaag gcgttggttt attccgcact gaaatgctct atatggatcg
    17221 caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc tggagtccgc
    17281 caacggacgc agcattattg tgcgcactat ggatattggc ggtgacaaac ccgttgatta
    17341 tctgaatatt cccgcagagg caaacccgtt cctcggttat cgcgcggtgc gtatttatga
    17401 agaatacgcg tcgttgttta ccacacaact acggtcgatc ctgcgcgcct ctgctcacgg
    17461 cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat gggtgaaaga
    17521 aaaactggcg gaagccaaac agcaactgcg taacgaacac attccgtttg atgagaagat
    17581 tccactcggc atcatgctgg aagtgccgtc ggtgatgttc atcatcgatc aatgctgcga
    17641 agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctac tggcagtgga
    17701 tcgcgataac gctaaggtga ctcgtcacta caacagcctg aatccagcat tcttgcgggc
    17761 gctcgattac gccgtgcagg cggtgcatcg ccagggcaaa tggattggtc tgtgcggtga
    17821 actgggagcg aaaggttccg tgctgccgtt gctggtcggt ttagggctgg atgaactcag
    17881 catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg atagccgtga
    17941 gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag tggaacacct
    18001 gctggcgcaa ttccgcatga cccaacaaga cgcaccgctg gtcaccgccg agtgcatcac
    18061 actggaaagc gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg ataacctgct
    18121 gctggcgggc cgctgtcgct atccgcgtaa actggaagcc gacttgtggg cgcgcgaggc
    18181 cgttttctct accggtctgg gctttagttt tgccattccc cacagcaaat cagaacacat
    18241 tgagcaatcc accatcagcg tggcgcgtct ggcagcgccg gtgcgctggg gcgatgatga
    18301 agcgcaattc atcattatgt taaccctgaa caaacatgct gcgggcgatc agcacatgcg
    18361 cattttctcg cgcctcgctc gtcgcatcat gcacgaagaa ttccgtaacg cgctggttaa
    18421 cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac tgtaaaagga
    18481 aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg tctggcacgc
    18541 atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag caaggagtcc
    18601 atctgggaag tgctgccgcg ccttcaaaaa gcgatcggtg atgagggaat tctgtttgct
    18661 cagaccatga gccgcgacgc gcagggtatg gtgaaagaag cgaaacgcct gcgcgacgct
    18721 attccgggca ttgtggttaa aattccggta acctctgaag gtctggcagc aattaaaatg
    18781 ctgaagaaag aaggcattac tacgctggga accgcagtgt acagcgcagc gcaaggatta
    18841 ctggcggcgc tggctggagc caaatacgtt gctccatacg ttaaccgcgt agatgcccag
    18901 ggcggtgacg gcattcgtac tgtacaggag ctgcaagcgc tgttggaaat gcatgcgcca
    18961 gaaagcatgg tgctggctgc cagctttaaa acaccacgtc aggcgctgga ctgtttactg
    19021 gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct caacacccct
    19081 gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt tggcactact
    19141 catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat acatccaggg
    19201 cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac gctggttagt
    19261 ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa gctttaaaga
    19321 tgctggactg gtagtagaaa ttgcgccgtt tggcggtgaa tgttcgcaaa atgagatcga
    19381 ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcagta tcggtggcgg
    19441 aaaaactctc gatactgcca aagcactggc acatttcatg ggtgttccgg tagcgatcgc
    19501 accgactatc gcctctaccg acgcaccgtg cagcgcattg tctgttatct acaccgatga
    19561 tggtgaattt gaccgctatc tgatgttgcc aaataacccg aatatggtca ttgtcgacac
    19621 caaaatcgtc gctggcgcac ctgcacgtct gttagcggcg ggtatcggcg atgcgctggc
    19681 aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg cgggcggcaa
    19741 gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc tggaagaagg
    19801 cgaaaaagcg atgcttgccg ccgagcagca tgtagtaacc ccggcgctgg agcgcgtgat
    19861 tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcgggctgg cggcggctca
    19921 cgcggtgcat aacggcctga ccgctatccc ggacgcgcat cactattacc acggtgaaaa
    19981 agtggcattt ggtacgctga cgcagctggt tctggaaaac gcgccggtgg aggaaatcga
    20041 aaccgtagct gcacttagcc atgcggtagg tttgccaata actctcgctc aactggatat
    20101 taaagaagat gtcccggcga aaatgcgaat tgtggcagaa gcggcatgtg cagaaggtga
    20161 aaccatccac aacatgcctg gcggcgcgac gccagatcag gtttacgccg cactgctggt
    20221 agccgaccag tatggtcagc gtttcctgca agagtgggaa taacctactc caaactcccg
    20281 gcttgtccgg gagtttgaac gcaaaattgc ctgatgcgct acgcttatca ggcctacgca
    20341 atctctgcaa tatagtaaat ttacgtactt ttgtaggccg gataaggcgt tcacgccgca
    20401 tccggcacgc tgtgccagta atctgaaact tccaccttgt tcgggtgttt ttttatccgc
    20461 ttctggacat tttctccaca gaacaatcgt tagcccggaa ggtcgaatct gtgactctta
    20521 ttttcacgga cgaaacagat gaaagcttca ctggcgttat tcagtcttct gacggtattt
    20581 accagttact ccttaaaatc tcccgccgtt ccaccaaccg tggtacaaat ccaggccaac
    20641 accaatcttg ccatcgcaga cggcgccaga cagcaaatcg gcagcacgct attttacgat
    20701 ccggcgtatg tgcagcttac ctatcctggc ggtgatgtcc cgcaagaacg cggcgtatgt
    20761 tccgatgtgg tgatccgtgc attgcgcagc cagaaggtcg atttgcagaa actggtgcac
    20821 gaagatatgg cgaagaattt tgctgcgtac ccgcaaaaat ggcagttaaa gcgcccggac
    20881 agcaacatcg atcaccgccg ggtgcctaat ctggaaacct ggtttacccg ccacgataaa
    20941 acacgcccca ccagcaagaa ccccagtgac tatcaagcgg gcgatattgt ctcctggcga
    21001 ttagataacg ggctggcaca tattggggtg gtgtcagatg gcttcgcccg cgacggtacg
    21061 ccgctggtga tacataacat tggcgcaggc gcgcaggaag aagatgtact gtttagctgg
    21121 cggatggtgg ggcactatcg gtattttgct aaataagaga tctccccaca tgaagaaaaa
    21181 attcagatcc tttttacact gacggcttct tttttcttac ggctcaccag cgccagtgcc
    21241 agtacaatca gcacaatacc gctaccttca actgcccccg gattctcacc gagcagccac
    21301 caggaaaaga gtacgccgca aacgggaacg gccagtgtgc ttaagctggc aatactggca
    21361 ggcaagtttt tcaatacaaa caaccataag ctccacgcca gcgctgtcgc cagaatcgca
    21421 ctgtaggcca gcgcccagaa cacggtgggt tgccagtcaa tttcacgttg cggcaccagt
    21481 aaagcgacca cgctcatcac cagcgccgca tacagcatct gccaggatgt tagcgacaat
    21541 aaatccacgc gcggatggcg ggcatacaga cgtttagcaa caatcgcgct cgccccccaa
    21601 ctcacaccgg agaggattgc cagcatggcg cttttcatcg aagagaaatc gagttgccac
    21661 ggctgcaaca ccaaaaataa gccgaaagcg gcaatcagaa tcgcgaaata ttgtccgcgt
    21721 cgcaggcgtt caccgagaaa caacgcggcg aaaattacca cccagaacgg catggtatag
    21781 ctcaggattg ccaccttccc cgctcctccg ctgaccagcg cccactgcgc caggccgacc
    21841 atcccgcagg tttgtaacag ggcaatggct aaggtgtatt taaacggtgt cgggcgcatt
    21901 ccgcgaccac gtaataaaag gacgatgaat aaaacgagag cgccgaaaat gcagcgtaag
    21961 gcggtaaagt cgaaggcacc gatgtagctg gtgacttgct tcatgaaaat ccagctataa
    22021 ctccagataa gtgtgagcac aaccaggcca ctgattgcca gtgggttgct ctttcctgcg
    22081 gcagacatga gagatccagt gtgtagtttt cctgtggaca ctatacgccg gtaaagaaac
    22141 ttcagcgact gcatgaaagg cagtcgctga agaaggtcag attacagcag gtcgaaacgg
    22201 tcgaggttca tcactttcac ccacgctgcc acgaagtctt taacaaactt ctcgtgggca
    22261 tcgctgctgg cgtagacttc cgccaccgca cgcaggacag agttagaacc aaagaccaga
    22321 tccgcacggc tggctgtgta tttcacttcg ccagtttcgc ggtcacggcc ttcgaacagc
    22381 tctttcgatt cgtcggtcgc tttccactcg taacgcatat ccagcaagtt cacgaagaag
    22441 tcattgctca atacgccaac gcggtcagtg aagacgccgt ttttgctgcc atcgaagttg
    22501 gcacccagta cgcgcatccc gcccaccagc gcagtcattt ccggcgcggt cagcgtcagt
    22561 tgctgtgctt tatcaatcaa cagtgactcg gtggtggaaa cgtccagacg agcgcgatag
    22621 ttacggaaac cgtcagcaat tggctccagc agttcgaaca tctcaatgtc agtctgatcc
    22681 tgacgcgcat caacgcggcc cggcgcaaac ggtacatgaa tgctcaaacc tgcggcgctt
    22741 gcggctttct caacgccaac cacacctgcc agcacgatga tatccgccag cgaagctttg
    22801 ccagactctt tctgaatttt ctccagaact ggcagagcac gaacggctgc ggcgttcaca
    22861 tcccagtcac gctgcggcat taatgccaga cgcgcaccgt tggcaccacc acgtttgtcg
    22921 ccaccacgga aggtagaagc agacgcccag gccaccgata ccagctcact aacagacaga
    22981 ccagaatccg caatcgcgaa tttcagatcg ataatgtcct gctcggtcgg gttgtagatc
    23041 ggctgcggca gcggatcttg ccagatcaga tcttctttcg gtacttccgg cccgatgtaa
    23101 cgagatttcg gccccatatc cctgtgcgtc agtttgaacc aagcacgagc aaaggcttcg
    23161 ttgaacgcct gcggatcgtt gaggaaacga cgagagatct tctcgaactc aggatcaaaa
    23221 cgcagcgtca ggtcggtcac cagcatcgtc ggtttacgtt tcttcgacgg atcaaacgga
    23281 tccgggataa tttccggtgc gtctaccgct tcgaactgga ttgcgccagc cgggctgcga
    23341 gtttgtaccc actcatattt gaacaggttc tcgaagaaat agttgctcca ttgggtcggc
    23401 gtctgggtcc agactacttc cagaccagag gtaatggcat ctgcgccaac gccgctgccg
    23461 taagtgctcg cccagcctaa accttgttct tcaatcggtg cagcttctgg atcaggacct
    23521 acatttgatg tcggaccggc accgtgagtt ttacccagcg tatgaccacc cgcaatcagc
    23581 gccacggttt cttcgtcgtt catgcccatg ttgccgaagg tcgcgcggat agctgctgcc
    23641 gcagaaagcg gttcgccgct gtgatccggg ccttccgggt taacgtaaat caggcccatc
    23701 tcggttgcgc ccagcggtgc tttcgccagt gcttccggat gacggtgggt cagccaggct
    23761 ttttcatcac cccagttaac atccagatcc ggttcccaga cgtcttcacg accggcacca
    23821 aaaccgaagg tacggaagcc ggagttttcc agcgccacgt tacccgcgag gataaacagg
    23881 tcggcccagg agattttctg accatatttc tgtttgattg gccacaacag gcggcgcgct
    23941 ttatcgaggc ttacgttatc cggccaggag ttcagcggtg caaaacgttg ctgaccacga
    24001 cccgcgccgc cgcgcccatc gattgaacgg taagtacccg caccgtgcca ggccatacga
    24061 ataaacagac cggcgtagct gccccagtcg gcaggccacc acggttgaga ttctgtcagc
    24121 agggctttca gatctttttt cagtccgtag taatctaatt tgctgaattc tttgcggtag
    24181 tcaaagtcct cacccagcgg gttagaacga ttagaatgtt ggtttaacag gtcaacacgg
    24241 agttgatttg gccaccagtc gcgcgtggtt gtgcccccac ctgcgctctg gtcgtgaccg
    24301 ccctgatgga acgggcattt gccggtggct gtcgtgttat ggatatcgtc tgacgtgctc
    24361 atcaatgtgc tcccctctac agtgttaccg ttacgataca cagcgttaga gagaagttat
    24421 aattgaatta atccacggat gcgatagttg agatcttaca tattgttggt taaagagatg
    24481 tagatcaaat tgatcgtaat taagactttt tgattatgtc cgatttagga caaatgcttt
    24541 ataaaaaagg gttttagatt tgtatccttc aagtaatgag ggagaaaaca aggctgataa
    24601 ccttattttc ccgccctcat ttcgaggcag cattttgtgc tctgtttaaa atttgtgatc
    24661 actgtgtaat tttcacaaaa ggcacactat ttataaacca ggtcgaacac ccagtgtatg
    24721 gcaaatcgcg taactcattt cagcgcggtt aagcgtatag aagtggaaat ctttcactcc
    24781 ttcacggctt aaaatcttca ccatatccat ggcaatattc gcgcccacca gcttgcgggt
    24841 ttcagcatcg tcatccaggc cgtcgaacat ttgtgccatc cacgccggaa tacgcacgtt
    24901 ggtcatgtcg gcaaatttct tcgcctgttt aaagttcgaa accggcaaaa ttcccggaat
    24961 gatttccaca tcaatgcctg ccgagacgca gcgatcgcga aaacgcaggt agctttcgac
    25021 atcgaagaag aactgagtaa tggcgcggtt ggctccggca tccacttttc gtctcaggtt
    25081 gagtaaatcg gcctgggcac ttttcgcttc tggatgtact tccggatacg ccgccacgga
    25141 gatatcgaaa tctgccactt cttttaacag tgtcaccagg tcagaagcat acatttctgg
    25201 cttaccactt cccggcggca ggtcgccacg cagcgccacg atatggcgaa taccgttatt
    25261 ccagtagtcg cgtgcaatgg tccgcagctc ggcaggcgtc gcatcaatac aggtaagatg
    25321 cggtgccgct tccaggccgg tgcgatcttt aatgccttta ataatgctgt gcgtacggtc
    25381 gcgctcgccg gagtttgcgc cataggtcac cgatacaaac ttcggtttca ggctgctaag
    25441 gcgatcgatg gagttccaca gggtctgctc catttcactg gtacgcggcg ggaaaaattc
    25501 gaacgaaacg ttaatctgcc cctggacttc tgccagactc tgattcaggg catcccgctg
    25561 gctggcgtga aaaaagctca taccttacct catcaatcgc ttatcgttat atgttgtgtt
    25621 gtgcacatct atccgtttag acgtccagat gtaaagatga aggaaaagcc gaagggcgtc
    25681 aaccgaaaaa taaccaatga tggtgaggaa aaatcaggga agatgaaaaa aactcatctt
    25741 aattagaggc gataaaagat ttctctatag cacaatccac ctcgcacgtt ttgactcata
    25801 ttctgacaac acagcgacac ctgggctaat tcgaaaaaca acgtcctgcc accggatggt
    25861 gatgctaagg catctgattc ggcctgcgcc ctcgcgtagg ccgaatacag gatctgcgca
    25921 atcaacgggt aacaaacagt tgagtcaatt cagtgcgcac ttttatttga tatccttcac
    25981 gcgcataccg ttgagctgtt catccgtaat ggtgttgccc gctttgaagt aatcaaccac
    26041 cgcgtgataa acgtaataac cgcccgttgt gttacgcgct ttcccttcgg taaaggcggt
    26101 aaaaccatct ccaccatcag caaggaaact ctgagtagca atgtgataaa ccgtcgcatc
    26161 ttcaatgggt ttgccattca gagtcagtgt gattacccgc tgaccaaccg gcttgctgct
    26221 gtcgtacttc atttccaggc ctttcgatac ctgtaaaacg ccattgctta aacttgcgcc
    26281 gtgttccatc agactgcgta attgtttacc cgtgagttcc atcgtcacca gttcgttggg
    26341 gaacgggaac gtactgatga cgccgcccat cgtgatcgcc cctgcgggga tctcattgcg
    26401 aatgccgcca gagttggtta acgccaattg ggtgttttta cccgccgcta ccagtaaagc
    26461 gtcagccgcc aggttcccga gggaagccga ttcaccatag gcacgtttta attcaaccgg
    26521 cgattgcgcc accgtttgtt gcaccacttc atcaagcttt ttattccagc cgtcgatcac
    26581 ctgtttcgtt tgtggatcgg gcttccactc gtcagcgtaa atggttttaa gctcgaagtt
    26641 tttcaccgta aaattatgcg gcttctcttt gtagtcgaga accagtttgc ccacatcaat
    26701 cccgccgctg tcagtggaga ggatgagcgt attgccgact ttaatcggtt ccggcgtacc
    26761 cacatgtgca tgtccggtga tcaaaatatc cagccctttc acctgacttg ccgtctgaat
    26821 atctttatcc agcgcgcgac gcacatctgt gcctcccata ctggactggc gggccggaac
    26881 accttcatgg atcagggcga cggtcaaatc aaccttgcct ttaaattcat cgatataacg
    26941 ttgtagccat ttaatttcat cacgcgcctc aatacccact cgcgtggcag cagatactgt
    27001 gtcattaaag gcaaatacac cgtgcaaacc tataacgcct attttcacac cgtctttttc
    27061 gatgatggta tagggcttat cccagaatga tttactgctg ttctgataaa aaacattgcc
    27121 ctgcacaatc gggaattttg cctgactcaa ctgtaataat gtgttgtccc agccgtggtc
    27181 aaattcatgg ttgcctatcg tgacggcatc aaacggcata gtattcataa tatcgataat
    27241 agccttacct ttggtcaggc tgctgatata tggcccggta aaatagtcgc ccgcatcaaa
    27301 aaaccatgtc gctttatttt ttgctttttc ttgcttaacc agcgtagtga tatttgccca
    27361 gccgccaata tcccgtttac cgtcagcgat ccacggcact ttataaggtt ctacatgcgc
    27421 atggaggtcg ttggtataaa taatagtgac atctttagcg caagcccaga acggcagtgt
    27481 taatgcgata cccgcagcaa gtattttaat tttcataaac aatccctttc agtgagataa
    27541 aggattgcta cattacgttc tgaaagactt atttatgtga gagtaatcac atctgcaggg
    27601 gatagttgcc ggacaaaatc aacgaccttg tgaaatcgtt ggtgatatat gtcgactctg
    27661 tttacgatta atgcctgtaa aacgttcggt tgccgaaatt tgggattggc ctcatcggaa
    27721 gattacagtt ggccagacta taaactgggt tatccggcac tgcactgtcg ggcatgtggc
    27781 agttacccgc cgttgtttga cgagcaacaa tttcgtgact ggttgtcagt tcacttatct
    27841 acgtgggcct cggagaaggg tcatttctgc ccggtatgtt atggcacaga catgatttgt
    27901 tatggtcaca atccacaggg ttcgcaacgc attcaatgcc gtaactgcaa aaaagtctgg
    27961 acgcctaaac agtatcagaa agaaattacg catccccagg ccattgaaac cgttcaactg
    28021 ttcataccct ttcaaggggc cagcgcagta caaaaactgt atgttttagt cagtcttgac
    28081 gccactcgtg gcaacattct tcatctctcc acgaattata cgcaacatca gacaggagac
    28141 agtctgcggt acagttacaa gggcaacaca gaaccaacta tgcaccatcg cgatatcgtg
    28201 cagaaggtag atatgcgtga agcgcaattc ttgcgccgga gccagttcga tgaaattcag
    28261 tatggcagtg ctgtactcaa gcgcaacggt aagggagcca tactacgccc ggttatcacg
    28321 gcacacgggc atttcagaat actgaaaatc cgctatccag atgtcaaaac gcatattatt
    28381 tcacacgaat gttttctaag aggcgcaatt attacagcct gggcagatca gttccgccaa
    28441 caacaaggtg aactttggtt cgtagaagaa gaaatcagtg acagtaacgc tgatacgccc
    28501 tggcatttta agggaacgac ataccatggt tggtggcaaa atcagtggca gcgctgggag
    28561 caggggaata actgcaagat ggtctgccta ctcacgggag cctgcttaga aaggggcgca
    28621 aatgtttctc tggcaaccag tcgttgtttt atcacatggc tgacagacca acacgacttt
    28681 acccaaagcg cgttattatc cgcaggtcgc gtaacacaaa tgctgacctc actggcgtta
    28741 aaatacaatg aatcgctcac tccatcttgt tagtcaatac actcgacaaa cgaccataaa
    28801 ttaagagaga acactacctt tcatttaatt aaatatataa atgtatgacc gtgaaatata
    28861 atcatggtca atgtttaatt aataacatta tttgttacta agatcacaca atacttttct
    28921 gaactatgct tacataaaat aagacacgat gtgtctttta tttaaataat tcaacaaatg
    28981 taacacaccc tatccaggag ggatgatgaa aactgtattt acaacattat cagtcatatt
    29041 cggcgtgata ttttctcaca gtgttctagc tcaggacgtt actatttatt atacgaatga
    29101 cattcacgca catgtcaatc cagcaaaaat catcgctgtt gataagaaca gacttatagg
    29161 tggtatggct aatatttcag gcatcgttaa tgaagcgaag aaaaaaagca aagatgtgtt
    29221 tttctttgat gcaggcgact attttaccgg gccgtatatc agcaccctga ccaaaggtga
    29281 ggctattatt gatatcatga acactatgcc ctttgatgcg gtgtctgttg gtaatcacga
    29341 gttcgatcac ggtgtgccca atatggtgtc tcagttatca aaagcaaaat tccccattct
    29401 gctgggaaat atctactaca ccgatacgaa taaaccagtt tgggatcatc cgtggaccat
    29461 tatcgaaaaa gatggtttaa agattggtgt gattggattg catggcgcat tcgctttcta
    29521 tgatacagtc gcggcaaaag cacgtgaagg cgtagaagcc agagacgaaa ttaaatattt
    29581 aaataaagca cttgcagaat taaaaggtaa agtcgacatt accgttttgc taattcacga
    29641 aggtgtcccg gcgcgtcaat caagctttgg tagtaaagat gtggagcggc tactacaggc
    29701 agatattgag acagctaaaa aagtgaatgg tgttgatgtc ttaattaccg gtcacgcaca
    29761 tgtcggaaca ccgcaaccaa taaaagttaa taacacatta attgtatcta ccgatgcata
    29821 cggcaccaac atcggaaaac ttgtgcttga ttacaatccg aaaacgaaga aaattgacag
    29881 ttataatggt gagttaatca ccatctttgc agatcaattt aaacctgaca ccatcgttca
    29941 aaatactatt gataaatgga gtgcaaagct taacaaaata acacaggaag ttgtcggcca
    30001 ttctcccgta gttttaacac gtgagtatgg tagttcctct tctaccggca accttattct
    30061 tgatgcaatg atggaaaaaa cacctgatgc cgttgccgga tttcaaaata gcggtgggat
    30121 tcgagctgat tttcctaaag gcgatatcac actgggagat gttattagca cattcccctt
    30181 taataatgac ctcatcgaga tggatttgac gggccgcgat ctcaaatcat tgatgacgca
    30241 tgcaaccaat ctaactaacg gtgtgttaca agtttctaaa agtgttgcgg ttgtctatga
    30301 cagcaaaaaa cccctcaacc aacggttaat ctctttcacc attaacggca aacccgtgga
    30361 agataatcaa acatatcgta ttgccacgca ctccttttgt gccagtggtg gtgacggttt
    30421 tgaagcattt ttgaatggaa aaaatgtgaa gacgataccg ggaacaacct cggcggaatc
    30481 tatcatcgat tatttcaaaa atcataaacc tgtcacccca gacttaacta aacgagtcat
    30541 ggacgtcgcc aaataaaatc tcatttatca gtcaatttat ttaacaagga atgcgaatga
    30601 aaacagcgca gcgtacttta tttgccgcaa cattgctgac tattttttcg acctcagtta
    30661 tggcgcagga tgtcacgatt atttatacca atgatcttca cgctcatgtg gattcctata
    30721 aagtaccgta tgtcgcagac ggaaaacgcg acattggtgg ctttgccaat atatccacgt
    30781 tagtaaaaca agagaaagcc aaaaataaag ccacctttta ttttgatgct ggagactatt
    30841 tcaccgggcc atatatcagt agcctgacaa agggcgaagc aataattgat attatgaata
    30901 ccatgccttt tgacgcagta tcaatcggta atcatgagtt tgaccatggc tgggataatg
    30961 ccctccgaca attaagcaaa gcgaatttcc ctgttttatt agccaatgtt tatcataaag
    31021 agagtgagaa acccttctgg aacaagccct acaccatcct ggaaaaagat ggcatcaaga
    31081 ttggtattgt tgggctacac ggcgtatttg catttaatga cacggtctcg gagctctctc
    31141 tccagggact cgataatgac aataataacc gatttgataa atcttccgcg accctcaaga
    31201 atcaggggat tgaagcacgt gatgaagtga agtatttgca gcattatatc gatgagttac
    31261 gtgataaggt tgacctcacc gtggcgctgg ttcatgaagg cgtaccggcg cgtcagtcca
    31321 gcattggcaa tacagatgtt agacgcgctc ttgataaaga tattcaaacc gcaagcaagg
    31381 tgaaaggatt ggatattctg attaccgggc atgcccacgt cggaacgcca gaaccaatca
    31441 aagtcggcaa caccttaatc ctttcgactg acagtggcgg tatcgatatt ggtaaattaa
    31501 tccttgatgt caaccctaca gctcgtactc ataaagtgaa gagctttgag ctgaaaacgg
    31561 tttatgccga tgaatggata cctgatccaa ccacacaaaa agttatcaat ggctggaata
    31621 aaaaactggc ggatcttgta cgccaaccag tcggtgaatc atcgattgca ttaactcgtg
    31681 cctacggtga gtcatcgcaa cttggcaacc tgtttaccga tgcaatgctt gtcgctgcac
    31741 cgactgcaca gattgcattg ataaactccg gcagcttacg tgcggatata aatgcaggca
    31801 cgattacctt tggcgacatt accagtacat tccccttcaa gaatgaactt actgaaatgg
    31861 atctcagcgg taaggatctg cgtaacctac tggaacacgg ggcatcgctt accaatggca
    31921 tcctgcaaat gtcaaaaggc gcagaaatgc gctatacccc gaagaaacca gtaggtcagc
    31981 gcatagtatc ttttaaaatt aatggtgaag agatcgttga tacgaatatt taccatgttg
    32041 caacaacgac tttccttgca ctggggggcg atggttttct ggcattcaaa gaagggaaaa
    32101 atgttcaggt ccgcgccgga aataacatgt ccgatgtcgt gatcgattat ttgaagaaag
    32161 gccacaagat tacgcctgca caggtaaatg aaatgcgggt ggacgttagc aaataataaa
    32221 aaaattataa tgccattcat tcactgaatg gcattatttt tctctaaatc aacaataaat
    32281 aatgagatat atttatgtgt ataaaaaagc acgggatagc tttatccatt ctattaatac
    32341 cttgcattgg aaacgctcag gaaattaaaa ttgatgaaag ctggttacat caaagcttga
    32401 atgtcattgg tcgcacagac tctcgctttg gcccaagact gactaacgac ctctaccctg
    32461 aatatactgt agcaggaaga aaagactggt ttgattttta tggttatgtt gatctaccaa
    32521 aattctttgg cgtcggcagt cactatgatg ttgggatctg ggatgagggc tcaccactat
    32581 ttacggaaat agaacctcgg ttttccattg acaaattgac cggattaaat cttgcgttcg
    32641 gcccttttaa agaatggttc attgcaaaca actatgtcta tgatatgggt gacaaccagt
    32701 catcccggca aagtacatgg tatatggggc ttggtacaga tatcgacacg ggtctaccaa
    32761 ttaagctttc tgccaatata tacgccaagt atcagtggca aaactatggt gccgctaatg
    32821 aaaatgaatg ggacggatat cgattcaaaa taaaatatag cattcctctt acccatttat
    32881 tcggagggcg attggtatac aatagtttta ctaactttga ttttggctcc gatcttgcgg
    32941 acaaatcaca caataataaa cgaaccagta atgctattgc ttcaagccat atcctttccc
    33001 tgctatatga acactggaaa tttgcattta cactacgtta ttttcacaac ggtggacaat
    33061 ggaatgctgg ggagaaagtt aacttcggag atggtccatt tgaattaaaa aacacaggat
    33121 ggggaaccta tactactatt ggttatcaat tttaaatcag gtgatgtttt agatattttt
    33181 acttaatgcc attttgtaat tgtcataaat aacttcacca ttcacactgt gaattcatcg
    33241 ctcaacacaa aagccccctt attaccgggg cactgaccaa aggcaaaatg acaatcaatc
    33301 accacctccg caggaggtgg tttagaggtg acaacaaaag ctggcaactg ccagcttttg
    33361 ttcaataatc actacaacaa ctgcgccaga cggttaatat ccgactgaat cgccccggcg
    33421 gtgacgtcgc gccctgcacc tggcccgcgg atcaccaacg gattatcgcg ataccagcgg
    33481 ctttcgatgg caaagacgtt atcgcacggt agtagtgatg ccagcggatg gtcttctcgc
    33541 accgcttcca cgcctactcg cgctttgccg ttggcatcga aacgcgccac gtagcgcaac
    33601 accagcccca tttcacgggc cgcttccaac cgttgtacca tctgctcatt aagttcatcg
    33661 ccattttcaa agaaatggtc gatgcttcca ccttcgcaat gggttggcac cagtgattca
    33721 acacgcacct ggtccggttc aatgttgtaa cccgcttcac gcgccagaat caccagcttg
    33781 cgcatcacat ctttgccgga gaggtcatca cgcgggtcag gttcagttaa gccctgctgc
    33841 cacgcctgat ccaccagctc agtaaacggc acgctaccgt cgaattgcag gaacagccag
    33901 gagagcgtgc cggagaagat cccgctgatc gacaaaatag tgtcgccgct gtcgatcaga
    33961 tcgcgcacgg tatggttgat cggcaaacct gcgccgacgg tggcgttgta cagccagtga
    34021 cgcccggttt tttcgaacgc gtcgtggatc tggcgatatt tattgctgtc gctcgctccc
    34081 gccagtttgt tggcactgat gacgtggaaa ccgtggctgg cgaaatcgag atactgatca
    34141 gcaagctgct ggctggcagt aacgtccagc acaactaaat catcatacgg atgggcacgc
    34201 atccacagga atagcgactc ttcatcctgt tcgacggctt catcattaaa gaaggctagc
    34261 gcacggctgg cgtccagccc gtcatagctc aacaggctgc gacggctgtc caccacgcct
    34321 gccagcacaa attcaaagcc ggtgcgcgcc gagagcgtgc tctgctcacg ggcgaacagt
    34381 tccagccagc gggaaccgat atttccctta ccgaacaata ccaggccgat gcgtttttct
    34441 gcgcggaaga cggactgatg cagcccctga atcaggcttt cggtcgggcc ggtgcgcagt
    34501 actgccacca ggctgatgcc gtcatcggac tgccaggtaa actcgaccgg ctggcctttc
    34561 agttgctgcc agaagcggtg gcaatgcagc gggttacggg tgacgcccgc cccaaccatc
    34621 gccaccagcg ccagcccctg acgcagacgc agttcgccgg gtaatcctgc ttcgtcgagg
    34681 attttcagcg cactgtcggc cacttccgag gtgtagcaaa attgcagcaa ctggcgatcg
    34741 ttatgcacgc caactgccag tgggcgcacc tgagcacgtt tcaggatttg gtcgatctct
    34801 ttatgcgcca gtttaaaatc ctgactggcg ggcacctgaa actcaatcaa acagacatca
    34861 tcgtggctgg tgacaatacg cgcaccagta ccggaggcca gcacgcgttc aatgcgcgtg
    34921 gaaccttgat ccggcgtgta gctacagcgc agctgcaggt cgatttcact accggatact
    34981 ggctgtaaag tacgggcgtg aagaacgggg gccgccagac gcgccagttc gctggcttca
    35041 tcaagacgca gcaatggcag cagacaggca tctttcactt tacgcgggtc ggcactatat
    35101 accccggcga catcgctcca gatggttacg cgggaaacac ccgccagcgc accgatttgt
    35161 gtcgcggaat agtcggaacc gttacgcccc agcagcaccg tttcaccggc gttgttacgg
    35221 ctgataaacc cggtcaccac cagacgtttg cccggatgtt gtaccagtag ctgttgcagc
    35281 aacgggtaag aaagcccttc atcaacctgc ggctgcgcgg cgcgttcagc gcgtaaaaac
    35341 tcgcgggcat caagccaggc cgctggcagc ccttgttgat taagtaccgc agacatcagg
    35401 cgcgccgacc acacttcacc gtggcccacc acttccgcat acaccgcatc attaataccg
    35461 ctgtcgagca gcgccgcgag gcgttccaga tcgctgacaa aggcgctaat gaggctatcg
    35521 gcttcttcgg cgggtagcag gccgctaatc agatcgcact gataacgacg tagcgtttgt
    35581 tgaacctgat gcgcagagag acgatcggtc tggcttagtt tcaaccagtt aatcaactgg
    35641 ttagtggtgc taccggcggc ggaaaccacc atcatatcgt caggctgaga gtactccgcc
    35701 ataatgcccg cgacacgcaa ataacacttc acatcagcca gactactgcc accaaattta
    35761 tgcagttgac gacctttcgc ccctgcctgc gcaatcacac tcatttttac cccttgtttg
    35821 cagcccggaa gccattttcc aggtcggcaa ttaaatcttc gccatcttca ataccggtgg
    35881 agatacgtag cagcgtctcg gagatcccgg cggcagcacg cgcttctggc gccatgcctg
    35941 catgtgtcat ggttgcggcg tgagagatta aactttccac tccccctaat gattccgcca
    36001 gcgtaaacaa cgacaacccg cccaggaaac gacgcagcgt ctgctcatcg ccatccagtt
    36061 caaaactcaa cattgcgcca aagccttttt gctggcgcgc ggcaatttca tgcccctgat
    36121 tttccggcag cgacgggtga tacagttttt tcaccaacgg ctgggtttgc agatatttca
    36181 caatcgcctg cgcgttgcgc tgcgccagct ccatacgcgg caccagcgtt cgcaacccgc
    36241 gcagcagcag atagctgtca aacgcgccgc ccgtcacgcc aatattgttt gcccaccagg
    36301 caagttcagt gacaacgtcc gggtctttag caatcactac gccggccacc acgtctgagt
    36361 gaccgttcag atatttcgta catgaatgca acaccagatc ggcacctaat gccagcggat
    36421 tttgtaatgc cgggcttaag aaggtgttat ccaccacgct caccgccccg acttcccttg
    36481 ccagatggca gattttcgca atatccacga cgcgtaacaa tggattactt gggctttcta
    36541 ccagtaccag tttgggtttt tctgccagcg ctacccgtaa tgcctgttca tcgccttgat
    36601 caacaaacaa cacgcgatag caaccgcgtt tcgccagact gtcgaacagg cgatagctgc
    36661 cgccatagca gtcgtgcggc gcaaccagca gatcgcccgg tttcaaaaag acggtcgtta
    36721 ccagatgaat cgcggacatg ccggtattag taagtacggc accagcaccg ccttccagtt
    36781 ctgccagcgc acgctgaacc acatcgcgcg ttgggttgcc gcgccgcgag taatcatgcg
    36841 cgcgcggttc attaaatccg gtaaagttat aggtgctgga aagatggatc ggtgggacaa
    36901 cgcaaccata ctgttcgtcg tcatttaacc cgctacgcac tgcgatggtg gcctgtttac
    36961 gcgtcatgtg atgaagttcc ctgggcttgg tcggtgaaat gtcaggcacc agagtaaaca
    37021 ttgtgttaat ggacgtcaat acatctggac atctaaactt ctttgcgtat agattgagca
    37081 aatcccaaat agccgttaaa attatatgca ttatcacgcc gacaggtgca ttacacgatg
    37141 tcacggtagc gcctgtacgg taaactatgc gggtttacgg tcagtaccca cataaattgt
    37201 gtggtctggt ctcaatttat tgacgaagag gattaagtat ctcatggctg aatggagcgg
    37261 cgaatatatc agcccatacg ctgagcacgg caagaagagt gaacaagtca aaaagattac
    37321 ggtttccatt cctcttaagg tgttaaaaat cctcaccgat gaacgcacgc gtcgtcaggt
    37381 gaacaacctg cgtcacgcta ccaacagcga gctgctgtgc gaagcgtttc tgcatgcctt
    37441 taccgggcaa cctttgccgg atgatgccga cctgcgtaaa gagcgcagcg acgaaatccc
    37501 ggaagcggca aaagagatca tgcgtgagat ggggattaac ccggagacgt gggaatacta
    37561 aagcggaaaa gagcggcgcg gagttaacca gccgcttttt tcatacatgg acgtttaacg
    37621 atgaaaatca ggctactgat cctcagcctg ctggtttctg tgcctgcctt tgcctggcag
    37681 ccgcaaactg gcgacatcat ctttcaaatc tctcgctcat cgcaaagtaa agcgatccaa
    37741 ctggcgaccc attccgatta cagccacacc ggtatgctgg tgatacgcaa caaaaagccc
    37801 tacgtttttg aagcggtcgg cccggtaaaa tacaccccac tcaagcagtg gatcgcccat
    37861 ggtgaaaagg gcaaatacgt tgttcgccgc gttgaaggcg aactgagtgt tgaacaacag
    37921 caaaaactgg cgcaaacggc aaaacgttat ctcggtaaac cgtatgactc cagcttctcg
    37981 tggagcgacg atcgccagta ctgttcggaa gtggtttgga aggtttatca gaacgcgctg
    38041 ggaatgcgcg tgggcaaaca gcaaaagttg aaagagttcg accttagtaa cccgcaggtt
    38101 caggcaaagc tcaaagaacg ctacggcaaa aatattccgc ttgaggaaac ggtggtctcg
    38161 ccacaagccg ttttcgatgc gccacaactc accacggtcg ccaaagaatg gccgctgttt
    38221 tcgtggtaat acgaccaaaa ttcaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga
    38281 aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc
    38341 accggtagca acatcacgct gtttgccagt gaagaacggg tggcacttgc tgcacacgtc
    38401 gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc aagagcagct
    38461 agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa
    38521 ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc
    38581 aatacgatca tttcgtatga aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac
    38641 tgatccgcac tcttctacgg caatgtgtat actaacccac cgaatttcaa gtcaggatga
    38701 tgctatgccc gttgcccacg ttgccttgcc cgttccgctt ccccgtacct ttgactatct
    38761 gctgccagaa ggcatgacgg tcaaagccgg gtgtcgcgtg cgcgtgccgt ttggcaaaca
    38821 gcaggagcgc atcggggttg tggtgtcagt tagcaatgtc agcgagctgc cgcttaacga
    38881 gctgaaagcg gtagttgaag tgctggacgt tgagccggta tttactcact ccgtctggcg
    38941 attactgctc tgggcggcag attactatca tcatccgatt ggcgatgtgc tgtttcatgc
    39001 cttgccgatt ttactacgcc aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt
    39061 tgccactgaa caaggtcagg cggtggatct gaacagcctg aaacgctcac ccaagcaaca
    39121 acaggcgctg gcagcgttac ggcaaggcaa aatctggcgc gaccaggttg ccacgctcga
    39181 atttaatgat gccgcgttgc aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga
    39241 aacaccagag tttagtgact ggcgaaagaa ttatgccgtt tctggtgagc ggttgcgatt
    39301 gaatactgaa caggccaccg ccgttggcgc aattcatagt gcggcggata ccttctctgc
    39361 ctggctgctg gcgggcgtta ccggttccgg taaaacggag gtttatctca gtgtactgga
    39421 aaacgtgctc gctcagggca aacaggcgct ggtgatggtg ccggaaatcg gcctgacgcc
    39481 gcaaactatc gcccgttttc gtgaacgttt taatgcgcca gtggaagttc tgcattccgg
    39541 cctgaacgac agcgagcgtc tttcggcgtg gttgaaagcg aaaaatggtg aggcggcgat
    39601 tgtgatcggc acccgctccg cgctgtttac gccgtttaaa aatctcggtg tgattgtcat
    39661 tgatgaagag cacgacagtt cctacaagca gcaggaaggc tggcgctatc atgcccgcga
    39721 cctggcggtt tatcgtgcgc acagcgagca aatcccgatt attcttggct ccgcaacacc
    39781 cgcactggaa acgttatgca acgtccagca gaaaaaatac cgcctgctgc gcctgacgcg
    39841 tcgggcgggg aatgcgcgtc cggcaattca acacgtgctg gatttaaaag gtcagaaggt
    39901 gcaggcgggg ctggctccgg cgttaatcac ccgtatgcgc cagcatttac aggccgacaa
    39961 ccaggttatc ctctttctta accgccgtgg ctttgcgcct gccttgctgt gccacgactg
    40021 cggctggatt gccgaatgtc cacgttgcga tcactactac acgctgcatc aggcgcaaca
    40081 gcatttgcgc tgtcaccact gtgacagcca acgcccggtg ccgcgccagt gcccttcctg
    40141 cggttccacg cacctggtgc cggtggggct gggcaccgaa cagcttgaac agacgcttgc
    40201 gccgctgttc cctggtgtgc ccatttctcg tatcgaccgc gataccacca gccgcaaagg
    40261 agcgctggaa caacaactgg cggaagtgca tcgcggcggc gcgaggatct tgatcggcac
    40321 gcaaatgctg gcgaaaggtc accactttcc ggatgtgacg ctggtggcgt tactggacgt
    40381 agacggcgcg ctgttttctg ccgattttcg ctcggcagag cgtttcgccc agctttacac
    40441 ccaggtcgcc ggtcgtgccg ggcgtgcggg taaacagggc gaagtggtgc tgcaaacgca
    40501 tcatccagaa cacccgctat tgcaaacgtt gctctataaa ggctatgacg cctttgccga
    40561 acaggcactg gctgagcgac gcatgatgca gctgccgccg tggaccagcc atgtgattgt
    40621 gcgtgcggaa gatcataaca atcagcacgc gccgctgttc ctgcaacaac tgcgtaatct
    40681 catcctctcc agcccgctgg cagacgacaa actgtgggtt ctcggtcccg ttccggcact
    40741 ggcacctaaa cgtggcggcc gctggcgctg gcaaattttg ttgcagcacc cttcccgcgt
    40801 gcgattacag cacatcatca atggtacgct ggcgcttatt aatacaatac cggattcccg
    40861 taaggtgaaa tgggtgctgg atgtcgatcc catcgagggc taatgcgctc acgatgcgag
    40921 gcggatcgaa aaattcaata ttcatcacac ttttcatgaa aattctgtaa ccgttttcac
    40981 gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt
    41041 gccaggcgag gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac
    41101 gttgccctca aggcaaaagt ctctacagcg actgtctccc gagcattaat gaatcccgat
    41161 aaagtctccc aggccacccg taatcgggtt gaaaaagcgg cccgggaagt gggttattta
    41221 ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc gcactattct ggtgattgtc
    41281 ccggatatct gcgatccctt ctttagcgaa attattcgcg gtatcgaagt tacggcggca
    41341 aatcacggat atctggtgct gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc
    41401 tttatcgatt tgatcatcac caagcaaatt gatggcatgt tgctgctggg ttcaaggctg
    41461 ccgtttgatg ccagcattga ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa
    41521 tttgcaccgg aactggagct gcctaccgtt catatcgaca atctgaccgc cgcatttgat
    41581 gcagtaaatt atttatatga gcaagggcat aatcggattg gctgtatagc cggtcccgaa
    41641 gagatgccgc tgtgtcacta ccgcctgcaa ggctatgtcc aggcgctgcg tcgctgcggc
    41701 attatggttg atccgcaata catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa
    41761 gcgatgcagc agttgcttga tcttccacaa ccgcctactg ctgtcttctg ccatagcgat
    41821 gtgatggcgc tcggcgcact ttctcaggca aaacgccagg ggctgaaagt cccggaagac
    41881 ctttccataa tcggttttga taacatcgac ctgacgcaat tttgtgatcc gccgctgaca
    41941 accatcgcgc agccgcgtta cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa
    42001 atgcaggggc aacacgttgg cagtggctct cgtttaatgg actgcgaact tatcatccgg
    42061 ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc
    42121 ccgccgctta agtaacatgg cgggctgacg aacgaataaa tacagcgaaa cgatagtggc
    42181 acaacgagat tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc
    42241 acggaaaaag caacgaaatc tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc
    42301 cgttcttgtg acctttatcg gtggtctgta cttcattacg catcacaaga aagaagagtc
    42361 cgagacactg caaagccaga aagtgaccgg aaacggacta ccaccaaaac cagaagaacg
    42421 ctggcgctac atcaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc ctacagaacc
    42481 ttctgccggt ggtgaagtga aaacgccgga gcaactgaca ccagaacaac gccagcttct
    42541 tgagcaaatg caggctgata tgcgccagca gccaacgcag ctggttgaag ttccgtggaa
    42601 tgaacagacg ccggaacagc gtcagcaaac gctacagcgc cagcgtcagg cgcagcaatt
    42661 agcggagcag caacgcctgg ctcagcagtc gcgtacgact gaacaaagct ggcagcagca
    42721 gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta aaccggcttc
    42781 tactcagcag ccgtaccagg atctgctgca aacccctgcg cacacgaccg cgcagtcgaa
    42841 gccgcagcaa gctgcgccgg ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa
    42901 aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga
    42961 gacggtacgt gctcagctgg cgttcgaagg ctttgactca aaaattacca ccaacaatgg
    43021 ctggaatcgt gtggtgattg gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa
    43081 tcgcttgaag atagcgggtc atacaaactg cattcggctc gccgccgggg gttgaaaccc
    43141 tcaaaatccc ccccatctat aattgcatta tgccccgtac ttttgtacgg ggtttgtact
    43201 ctgtattcgt aaccaagggg tcagctcgtg acaactatag taagcgtacg ccgtaacggc
    43261 catgtggtca tcgctggtga tggtcaggcc acgttgggca ataccgtaat gaaaggcaac
    43321 gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg cgggctttgc gggcggtact
    43381 gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca tcagggccat
    43441 ctggtcaaag ccgccgttga gctggcgaaa gactggcgta ccgatcgcat gctgcgcaaa
    43501 cttgaagcat tgctggcagt cgcggatgaa actgcatcgc ttatcatcac cggtaacggt
    43561 gacgtggtgc agccagaaaa cgatcttatt gctatcggct ccggcggccc ttacgcccag
    43621 gctgcggcgc gcgcgctgtt agaaaacact gaacttagcg cccgtgaaat tgctgaaaag
    43681 gcgttggata ttgcaggcga catttgcatc tataccaacc atttccacac catcgaagaa
    43741 ttaagctaca aagcgtaagg atctcccatg tctgaaatga ccccacgcga aatcgtcagc
    43801 gaactggata agcacatcat cggccaggac aacgccaagc gttccgtggc gattgctctg
    43861 cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt gaccccgaaa
    43921 aatatcctga tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg tcgcctggct
    43981 aagctggcga atgcgccgtt catcaaagtt gaagcgacca aattcaccga agtgggctat
    44041 gtcggtaagg aagtggattc tattattcgc gatctgaccg atgccgccgt gaaaatggta
    44101 cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag aactggcaga agaacgtatt
    44161 ctcgacgtac tgatcccacc ggctaaaaat aactggggac agaccgaaca gcagcaggaa
    44221 ccgtccgctg ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct tgatgacaaa
    44281 gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg aaattatggc tcctccgggc
    44341 atggaagaga tgaccagcca gctgcagtcc atgttccaga acctgggcgg tcagaaacaa
    44401 aaagcgcgta agctgaaaat caaagacgcc atgaagctgc tgattgaaga agaagcggcg
    44461 aaactggtga acccggaaga gctgaagcaa gatgctatcg acgctgttga gcagcacggg
    44521 atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat
    44581 gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg cactgtttcc
    44641 accaaacatg ggatggtcaa aactgaccac attctgttta tcgcttctgg cgcgttccag
    44701 attgcgaaac cgtctgacct gatcccggaa ctgcaaggtc gtctgccaat ccgcgttgag
    44761 ctgcaggcac tgaccaccag cgacttcgag cgtattctga ccgagccgaa tgcctctatc
    44821 accgtgcagt acaaagcact gatggcaact gaaggcgtaa atatcgagtt taccgactcc
    44881 ggtattaagc gcatcgcgga agcggcatgg caggtgaacg aatctaccga aaacatcggt
    44941 gctcgtcgtt tacataccgt tctggagcgt ttaatggaag agatttccta cgacgccagc
    45001 gatttaagcg gtcaaactat cactattgac gcagattatg tgagcaaaca tctggatgcg
    45061 ttggtggcag atgaagatct gagccgtttt atcctataat cgcgttcaat cattttcatc
    45121 attgtttgat ggggctgaaa ggccccattt ttattggcgc gtattatgac tgaacaacaa
    45181 attagccgaa ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc cctcgccttt
    45241 gctgcaatta tcgtcgggac ggcgctggca tggtggcaag gtcacttcga tccactggtc
    45301 gccctgctgg cattgattac cgccgggcta ttacagatcc tttctaacct cgccaatgat
    45361 tacggcgatg cggtaaaagg cagcgataaa cctgaccgca ttgggccgct acgcggcatg
    45421 caaaaagggg tcattaccca gcaagagatg aaacgggcgc tcattattac cgttgtgctc
    45481 atctgtcttt ccgggctggc actggttgca gtggcgtgcc atacgctggc cgattttgtc
    45541 ggtttcctga ttcttggcgg gttgtcgatc attgccgcta tcacctacac cgtgggcaat
    45601 cgtccttatg gttatatcgg tttgggtgat atttccgtac tggttttctt tggctggttg
    45661 agcgtcatgg ggagctggta tttacaggct catacattga ttccggcact gatccttccg
    45721 gcaaccgcat gcggcctgct ggcaacggca gtactgaaca ttaataacct gcgtgatatc
    45781 aatagcgacc gcgaaaacgg caaaaacacg ctggtggtgc gcttaggtgc agtaaacgcg
    45841 cgtcgttatc atgcctgcct gctgatgggc tcactggtgt gtctggcgct gtttaatctc
    45901 ttttcgctgc atagcctgtg gggctggctg ttcctgctgg cggcaccatt actggtgaag
    45961 caagcccgtt atgtgatgcg ggaaatggac ccggtggcga tgcgaccgat gctggaacgc
    46021 actgtcaagg gagcgttact gactaacctg ctgtttgttt tagggatatt cctaagccag
    46081 tgggcagcat aactgacaaa tatcaattaa caattgatga ttttgccaac agcctacata
    46141 gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt
    46201 ccgagctttg tgacatctat caagaagatg ttaacgtcgt ggaaccgctg ttctccaact
    46261 ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc gaggacaacg
    46321 ggttgctgta cgatctgctc gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg
    46381 gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg
    46441 aatgggaagg tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg gaagagctgg
    46501 atatcggcat ccaggcaatg gccgcaattc cggttggtgc cgctggcgaa ggcattggcg
    46561 aaagcgatgt ccgcgtcaat tttggcggtg taaccttctt ctccggcgac catctttacg
    46621 ccgacaatac cgggattatt ctttcagaag atccgctgga tattgaatga taaaaaaggc
    46681 accgcaaggt gccttttttc tgcgttacct gttggcctac acagtaaaga aattacgcga
    46741 aagatgaagc gtaatcagac ctcttccatg cgacccagca gggcctgcag acgttcctgc
    46801 cagccgttct gctgttcttt cagatggttg ttctcacgct ccagctcttc gcgctgatgc
    46861 tgggcatttt gaacttcctg cgacagtgag ttgttttttt ctttcagctc ttcgatttcc
    46921 atctgcaaca gagtgatggt atcaatcgcc tgctgtactt ttgcttccag tttctcaaac
    46981 acttctaatg acattgtcat acctctcctg aattgcaagg cgttgatgga taaaaaatcc
    47041 tcgtcccgat taccggtgac gccttaataa atacgagcgc actttagtta gctccgattg
    47101 tatgaagccg cgccatcgct gtccagcggc acgccttgca gattacggtt tgccacactt
    47161 ttcatccttc tcctggagac ataatccaca ccaatcgaaa atgttaataa atttgttgcg
    47221 cgaatgatct aacaaacatg catcatgtac aatcagatgg aataaatggc gcgataacgc
    47281 tcattttatg acgaagcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac
    47341 gataagttta cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca
    47401 aacattaact cttcaggatc cgattatgag tcaaacatca accttgaaag gccagtgcat
    47461 tgctgaattc ctcggtaccg ggttgttgat ttttttcggt gtgggttgcg ttgcagcact
    47521 aaaagtcgct ggtgcgtctt ttggtcagtg ggaaatcagt gtcatttggg gactgggggt
    47581 ggcaatggcc atctacctga ccgcaggggt ttccggcgcg catcttaacc ccgctgttac
    47641 cattgcattg tggctgtttg cctgtttcga caagcgcaaa gttatccctt ttatcgtttc
    47701 acaagttgcc ggcgctttct gtgctgcggc tttagtttac gggctttact acaatttatt
    47761 tttcgacttc gagcagactc atcatattgt tcgcggcagt gttgaaagtg ttgatctggc
    47821 tggcactttc tctacttacc ctaatcctca tatcaatttt gtgcaggctt tcgcagttga
    47881 gatggtgatt accgctattc tgatggggct gatcctggcg ttaacggacg atggcaacgg
    47941 tgtaccacgc ggccctttgg ctcccttgct gattggtcta ctgattgcgg tcattggcgc
    48001 atctatgggc ccattgacgg gttttgccat gaacccagcg cgtgacttcg gtccgaaagt
    48061 ctttgcctgg ctggcgggct ggggcaatgt cgcctttacc ggcggcagag acattcctta
    48121 cttcctggtg ccgctttttg gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg
    48181 caaactgatt ggtcgccatt tgccttgcga tatctgtgtt gtggaagaaa aggagaccac
    48241 aactccttca gaacaaaaag cttcgctgta atatgactac gggacaatta aacatgactg
    48301 aaaaaaaata tatcgttgcg ctcgaccagg gcaccaccag ctcccgcgcg gtcgtaatgg
    48361 atcacgatgc caatatcatt agcgtgtcgc agcgcgaatt tgagcaaatc tacccaaaac
    48421 caggctgggt agaacacgac ccaatggaaa tctgggccac ccaaagctcc acgctggtag
    48481 aagtgctggc gaaagccgat atcagttccg atcaaattgc agctatcggt attacgaacc
    48541 agcgtgaaac cactattgtc tgggaaaaag aaaccggcaa gcctatctat aacgccattg
    48601 tctggcagtg ccgtcgtacc gcagaaatct gcgagcattt aaaacgtgac ggtttagaag
    48661 attatatccg cagcaatacc ggtctggtga ttgacccgta cttctctggc accaaagtga
    48721 agtggatcct cgaccatgtg gaaggctctc gcgagcgtgc gcgtcgtggt gaattgctgt
    48781 ttggtacggt tgatacgtgg cttatctgga aaatgactca gggccgtgtc catgtgaccg
    48841 attacaccaa cgcctctcgt accatgttgt tcaacatcca taccctggac tgggacgaca
    48901 aaatgctgga agtgctggat attccgcgcg agatgctgcc agaagtgcgt cgttcttccg
    48961 aagtgtacgg tcagactaac attggcggca aaggcggcac gcgtattcca atctccggga
    49021 tcgccggtga ccagcaggcg gcgctgtttg gtcagttgtg cgtgaaagaa ggaatggcga
    49081 agaacaccta tggcactggc tgctttatgc tgatgaacac tggcgagaaa gcggtgaaat
    49141 cagaaaacgg cctgctgacc accatcgcct gcggcccgac tggcgaagta aactatgcgc
    49201 tggaaggtgc ggtgtttatg gcgggcgcgt ccattcagtg gctgcgcgat gagatgaagt
    49261 tgattaacga cgcctacgat tccgaatatt tcgccaccaa agtgcaaaac accaatggtg
    49321 tgtatgtggt tccggccttt accgggctgg gtgcgccata ctgggatccg tatgcacgcg
    49381 gggcgatttt cggtctgact cgtggggtga acgctaacca cattatacgc gcgacgctgg
    49441 agtctattgc ttatcagacg cgtgacgtgc tggaagcaat gcaggccgac tccggtattc
    49501 gtctgcacgc gctgcgcgtg gacggcggtg cggtggcgaa caatttcctg atgcagttcc
    49561 agtccgatat tctcggtacc cgcgttgagc gcccggaagt gcgcgaagtc accgctttgg
    49621 gtgcggccta tcttgctggt ctggcggttg gcttctggca gaatcttgac gagctgcaag
    49681 agaaagcggt gattgaacgc gagttccgtc caggcatcga aaccactgag cgtaattacc
    49741 gttacgcagg ctggaaaaaa gcggtcaaac gcgcgatggc atgggaagaa cacgacgagt
    49801 aataatgatt gcctgatgcg acgcttgcgc atcttatcag gcctacgagt cggatacaca
    49861 tccggcatca cgcagactct gaaaccgtgc gggggcaacc ccgcacacat caataatccc
    49921 gcccttcccc tgtgctacac ttcgcgccat tccttactgc ttagagtttg ctatgagacg
    49981 agaacttgcc atcgaatttt cccgcgtcac cgaatcagcg gcgctggctg gctacaaatg
    50041 gttaggacgc ggcgataaaa acaccgcgga cggcgcggcg gtaaacgcca tgcgtattat
    50101 gctcaaccag gtcaacattg acggcaccat cgtcattggt gaaggtgaaa tcgacgaagc
    50161 accgatgctc tacattggtg aaaaagtcgg tactggtcgc ggcgacgcgg tagatattgc
    50221 tgttgatccg attgaaggca cgcgcatgac ggcgatgggc caggctaacg cgctggcggt
    50281 gctggcagta ggcgataaag gctgtttcct caacgcgccg gatatgtata tggagaagct
    50341 gatcgtcgga ccgggagcca aaggcaccat tgatctgaac ctgccgctgg cggataacct
    50401 gcgcaatgtt gcggcggcgc tcggcaaacc gttgagcgaa ctgacggtaa cgattctggc
    50461 aaaaccgcgc cacgatgccg ttatcgcaga aatgcagcaa ctcggcgtgc gcgtatttgc
    50521 tattccggac ggcgacgttg cagcctcaat tctcacctgt atgccagaca gcgaagttga
    50581 cgtgctgtac ggtattggtg gcgcgccgga aggcgtggtt tctgcggcgg tgatccgcgc
    50641 attagatggt gacatgaacg gtcgtctgct ggcgcgtcat gacgtcaaag gcgacaacga
    50701 agagaatcgt cgcattggcg agcaggagct ggcacgctgc aaagcgatgg gcatcgaagc
    50761 cggtaaagta ttgcgtttgg gcgatatggc gcgcagcgac aacgtcatct tttctgccac
    50821 cggcatcacc aaaggtgatc tgctggaagg tattagccgc aaaggcaata tcgccaccac
    50881 cgaaacgctg ctgatccgtg gtaaatcacg caccattcgt cgcattcagt ccatccacta
    50941 tctggatcgt aaagacccgg aaatgcaggt gcacatcctc tgattgattt gatcgattga
    51001 gccttccagt ccttcgggac tggaattttt ttgttcggag aacgaagata aggcaagtca
    51061 atcaaaacag gagaaaaaca tggctgattg ggtaacaggc aaagtcacta aagtgcagaa
    51121 ctggaccgac gccctgttta gtctcaccgt tcacgctccc gtgcttccgt ttaccgccgg
    51181 gcaattcacc aaacttggcc ttgaaattga aggcgaacgc gttcagcgcg cctactccta
    51241 tgttaactcg cccgataatc cggatctgga gttttacctg gtcaccgtcc ccgatggcaa
    51301 attaagtcca cgactggcgg cactgaaacc gggcgatgaa gtgcaggtgg ttagcgaagc
    51361 cgctggcttc tttgtgctgg atgaagtacc agattgcgaa acgctatgga tgctggcaac
    51421 cggtacagcg attggccctt atttatcgat tctgcaattg ggcaaagatt tagaacgctt
    51481 caaaaacatg gtgctggttc acgccgcacg atatgccgcc gacttaagct atctacctct
    51541 gatgcaggaa ctggaaaaac gctacgaggg gaaactgcgc attcagacgg tggtcagtcg
    51601 ggaaacggct gcgggttcgc tcactgggcg attaccggcg ttaattgaaa gtggtgaact
    51661 ggaaagcgcg gttggcctgc cgatgaataa agaaaccagc catgtgatgc tgtgcggcaa
    51721 tccacagatg gtgcgcgata cacaacagtt gctgaaggag acccggcaga tgacgaaaca
    51781 tttacgtcgc cgaccgggcc atatgacagc ggagcattac tggtaagcgg ttacttgtcg
    51841 ataaacggca cgatcaagag atccgcactc aacttattga tcatcccgcg atatgccggc
    51901 atcaaacggt tgataaatga gtgatgatga ccacagacaa ggaggtcgca ctgctctttt
    51961 tgcataattt ccagcagcgt ttccggcatt tctccgcgtt caatacgcag ttttgtcttc
    52021 ggccattgaa tatttttcgt cagtttatac agcttgttat ccgacttatt cttcaacaat
    52081 tgaagaatat cttctgttgc agggaagtag atacccggat acaactcgct taagccatca
    52141 tcaatatgaa ttaacgtcag gtgagcgtca ttatgtctgg cgagctccag ggctttattc
    52201 accagtaagg catcttcttc attcccggaa attgccacgc caatgtgttt ataagccatg
    52261 tttaactcct tctaaagcaa ctccatcaag ctaacgaacg cagtgataac tcaaaaataa
    52321 tcatctcggc ctggcatgag aaggtgaagg ccgcatcaag ctcaacttgc ccctccagat
    52381 ccttaatctt ataggccacg ctggcccctt catctgcaga ggatgcagcg gcggcaagct
    52441 ctttcagttt ggtcataaaa gactctgctt cttcgcgggt ggcaaaaaag cgcgaaaact
    52501 tactggtaca gttgtcgtta tcaatcaccg taccgatatc aatcgcacat cctttagtac
    52561 tgcatttatc tacgacatct ttcatagggc acctctgtat attcgcctct ctgtcgatta
    52621 cgacttaaga ataatgcgcc gttaaaggaa gaacaaacaa gtaatgatct tatggattac
    52681 catttttttt gatattgctt aaggtgaaga gttcagaagg attttccggg cattgtcact
    52741 tgcatcgtgg aaataacaat cccggaaaaa aatagcgata agagatatat taaaggttaa
    52801 ttacttgatt tattgtcggc tttatacttc acatcctggg tatctttacc atatttattt
    52861 tcgccttgtg tgccaacaaa cgcaccaaga tcgataagca tcatcaccag aatcaatgtc
    52921 gggacaaaac gccccaccgc ccattgccag acacccggta aaattgccca gttacccgcc
    52981 agcagcatcc acgccacaat catcagaaat gcccatgcgc cggaacgccc gcgatcatgc
    53041 aagcgcttaa cggttactgc cgctgttggc caaagcaagc acacaaggca aaacgccgcg
    53101 gtctgaatat cgagtaaatt tttacccgcc agtgagaaaa gcaccagcat gcctgcgaac
    53161 cacaggccta tccaaatcca gaaatcacgg cgtccaatac gccctttaaa tgagaataac
    53221 cattgctgta tggtcatgta agttccttga tggttgtctt ttccaggatt ctaccctttt
    53281 gacaagggcg acagatatcg ttttaatcgg agccagtcat aacaaaaggt actgtcaatg
    53341 aagccagggt gtacgctgtt ttttctctta tgttctgcat taaccgttac aacaacggcg
    53401 catgcacaaa caccagatac ggcaacgacc gcgccttatc tgctggctgg agcccctact
    53461 ttcgatctct ccatcagcca gtttcgagaa gactttaaca gccagaatcc cagcctgcca
    53521 ctgaacgaat ttcgtgccat cgacagcagt cccgacaaag ccaatctcac tcgtgcagca
    53581 agtaaaatta acgagaactt gtatgcttct actgcgctgg agcgcggtac cttaaaaatc
    53641 aaaagcattc aaatgacctg gctacccatc caggggccag agcaaaaagc cgcgaaagcg
    53701 aaagctcagg catacatggc agcggtgatc cgcacactca ccccattaat gaccaaaact
    53761 caaagccaga aaaaactgca ggcgctacta acggcgggga aaaacaaacg ttattacacc
    53821 gagacagaag gtgcactgcg ttatgttgtc gcggacaacg gcgaaaaggg gctgaccttc
    53881 gctgttgaac cgattaagct ggcgctatct gaatcgcttg aaggtttgaa taaatgacaa
    53941 aaagcaaagc ctttgtgccg atgaatctct atactgtttc acagacctgc tgccctgcgg
    54001 ggcggccatc ttcctttatt cgcttataag cgtggagaat taaaatgcga catcctttag
    54061 tgatgggtaa ctggaaactg aacggcagcc gccacatggt tcacgagctg gtatctaacc
    54121 tgcgtaaaga gctggcaggt gttgctggct gtgcggttgc aatcgcacca ccggaaatgt
    54181 acatcgatat ggcgaagcgt gaagctgaag gcagccacat catgctgggt gcgcaaaacg
    54241 tggacctgaa cctgtccggc gcattcaccg gtgaaacctc tgctgctatg ctgaaagaca
    54301 tcggcgcaca gtacatcatc atcggccact ctgaacgtcg tacttaccac aaagagtctg
    54361 acgaactgat cgcgaaaaaa ttcgcggtgc tgaaagagca gggtctgact ccggttctgt
    54421 gcatcggtga aaccgaagct gaaaacgaag cgggcaaaac tgaagaagtt tgtgcacgtc
    54481 agatcgacgc ggtactgaaa actcagggtg ctgcggcatt cgaaggtgcg gttatcgctt
    54541 acgaacccgt atgggcaatc ggtactggca aatctgcaac tccggctcag gcacaggctg
    54601 ttcacaaatt catccgtgac cacatcgcta aagttgacgc taacatcgct gaacaagtga
    54661 tcattcagta cggcggctct gtaaacgcgt ctaacgctgc agaactgttt gctcaaccag
    54721 acatcgacgg cgcgctggtt ggcggtgctt ctctgaaagc tgacgctttc gcagtgatcg
    54781 ttaaagctgc agaagcggct aaacaggctt aagtctgaca ggtgccggat ttcatatccg
    54841 gcacttactt tccttaactc ctcgccttaa cgcaaaatct cacactgatg atcctggatt
    54901 tcctcggctg aagcacggtt aagcgtcagt agatttcgtt gtgtcgccag caatacaaat
    54961 gagttatcac tctgccgcac catcgccagc ccgtagcttc ccatatgttc ccgcgcctca
    55021 ggcacttctt ctgcaagcat cataaatggg ctgcgttgta ccagttcgct ttccgttacc
    55081 cgacgcgcca ggtattcatg cccgcgcaaa ccacctggca gtggcaacca gcggctgctg
    55141 atgttcgcca gattgttatc cagctgttcg cgcacatcag gacgaataca agagatatga
    55201 atatgaaaat ggttttgcgt acgcccggtg cgagagttga tcgccaaaga aaccgcgcga
    55261 tcgggaaccg gctggccgta ttttttgctc ataaaatcac gcgcctgcca ggccaaccaa
    55321 aagaagttcg gcgttgaagg atcggtcaac aaaggacttt cagtaccgtt aatacgatac
    55381 gttggcatca acagatattg cagtgggcca ttaagatctt ttaaaaccac gtatccggca
    55441 ttgggtttga cttccgcaca tggcgaagga ttttgatttt gctgctgatt gggcaaacat
    55501 tcctcaagga caatcttacg taatgtatcc gactcttcac cggttaattt ccagtaacca
    55561 ataccggcag ccacaacggc gataactatc atcaccaaaa aaagaagacc cgcttttttc
    55621 atcttttttc cctgtacctc aaagagatgc aagggtaacg caaaatcgtg acaaataaaa
    55681 aacccagtcg cgagaaacca ccgggtttga taattatcct ggagaaatca gcgtttgcta
    55741 atctgatcga acgtaccgcc gttagcaaaa tgctcttttt gcgctttcgt ccagccgccg
    55801 aactcttcat caatggtgaa taacttcagc tttggaaacg cattttcgta ctttttcgcc
    55861 acctcagcat cgcgcggacg gtagtagttt ttcgcggcaa tttcctgacc ttctggcgag
    55921 tagagatatt tcaggtaggc ttccgccacc tctttggtgc cttttttctc gaccacttta
    55981 tcgaccaccg acacggttgg ctctgcgagg atagactcac tcggcgtgac aatttcgaat
    56041 ttatctttcc ccagttcatt cgctgccagc agagcttcgt tttcccaggc aatcagtaca
    56101 tcgccaatcc cgcgctcgac aaaggtatta gtggagccgc gcgcgccaga atccagaact
    56161 tcgacgtttt tatacagtgc ccgaacaaaa tcctgtgctt ttgcctgatc gttgttgttg
    56221 tgatgcagcg cgtagcccca ggctgccagg tagttccagc gcgcgccacc agagcttttc
    56281 ggattaggcg tgatcaccga aacacccggt ttaatcagat cgttccagtc atggatctgc
    56341 ttcggattgc ccttacgcac caggaaaaca atggtggaag tgtacggtgc ggagttatcc
    56401 ggcagacgtt tgatccactc tttatcaatc cgcccgcgtt ccgcaatcgc gtccacgtca
    56461 taggccagag ccagcgtgac aacatcagct tcaataccgt tgattaccga cgtcgcttgt
    56521 ttgcctgaac caccgtggga ctgacggatc accacgttat cgccagtttg ctgtttccag
    56581 tgggcgctga ataccttgtt gtactgttcg tacaattcgc gcgttggatc atatgaaacg
    56641 ttaagaagct gaatatcctt tgccataacg ctggttgccg ccagcaaaaa tgttaaccct
    56701 acgccccact tgttcatcgc ccgactctct tatgttgtgt tgtgatgagc aaagcgtgcc
    56761 agaaggttaa ccaaacatta aagaataaaa aaagattggc tataacttgc gggtatatgt
    56821 agggggatta aaaaggcgga agaaatccgc ctcatattgc tgacaaagtg cgctttgtcc
    56881 atgccggatg cggcgcgaac gccttatccg gcctacaaaa gttcgcaaat tcaataaatt
    56941 gcagaattca tgtaggcctg ataagcgaag cgcatcaggc atttttgctt ctgtcatcgg
    57001 tttcaggcta aaggaatctg cctttttccg aaatcattaa tacagttttt tcgcgcagtc
    57061 cagccagtcg cctttgaacg gacgcttcat gttttcgata gcgtcgatga tgtcgtggtg
    57121 aaccagctgt tcgttctgga tacctacgca acgaccaccg taacctgcca gcagcagctc
    57181 gatagcgtaa gcgcccatac gggaagccag aatacggtcg taaggcaccg gagaaccacc
    57241 gcgctggatg tggcccagca cagttgcgcg ggtttcacga ccggtttctt tctcgatgaa
    57301 atgcgccagt tcgtcaacat cacacatatg ttcggtaatc gccactatcg cgtgtttttt
    57361 acctttcgcg atacccgctt tgatttcgtt taccaggtct tcacggctga attcaacttc
    57421 cggaaccaca acgaattcac agccaccggc aatagccgca gccaaagtca gatcgccaca
    57481 ataacggccc atcacttcca ccacggaaat acgctggtga gaagaagagg tgtcacgcag
    57541 acggtcgatc gcttctacaa cggtgctcag cgcagtgaag aaaccgatag tgtagtcagt
    57601 gcctttgatg tcgttgtcga tagtgcccgg cagaccgatg cacgggaagc ccatttcggt
    57661 cagacgcatt gcacccatgt aggaaccgtc accgccgata accaccagcg cgtcgatacc
    57721 acgttttttc aggttttcga tagccacggc gcggatgttc tcgtcgcgga attccgggaa
    57781 acgcgcagaa ccgaggaacg taccaccacg gttgatcatg tcagaaacgc tgtaacggtc
    57841 tagctgtacc atacggtctt catacagacc cagatagccg tcataaatgc ccattacttc
    57901 cagaccttct gtcagcgcag aacgaacaac cccgcgaatt gcggcgttca tgcctggcgc
    57961 atcaccgccg cttgtcaaca caccgatttt cttaatcatg actacctctg aactttggaa
    58021 tgcaaaatga aatctgttgc cggaagtctt cttgcacatc gaagtgatcc aacgaatgtg
    58081 caaatagtat aacaatcact tcctgctgaa ttgattcagg tcaggccaaa tggcggtatt
    58141 ttatacacaa aatgcgggtc tggctctctt ttatactgat tatgaaagca tagaccgttt
    58201 accctccctg ggtacgacgg aacaggggtc ctgatggata atcacatccg atcccggaaa
    58261 acgccgtaaa atagcctgct ctacctgatc cgccaccata tgtgcctgaa ccaaaggcag
    58321 agagtcttcc atttccaaat gaatctgaat aaagcgggtc ggccctgact gccgcgtgcg
    58381 aagatcgtga gcgccgctaa cacccggcca ggaagtcacg atatcaataa tttcttgccg
    58441 ttcttcatca ggcaatgcgc gatccagtaa tgactgtacc gcctcatatc ccatgcgtaa
    58501 cgcgctatat aaaatataga tgccgattcc caatgcaaac agagcatcgg cgcgatgcca
    58561 gccgtaccag gacaacccca gcgccagcag aattgcaccg ttcatcataa catcagactg
    58621 gtaatgtagc atatcagccc gcaccgcctg gctttgcgtc cggcgcacca cccaacgctg
    58681 aaacgagaca aggataatcg tacaaattag cgccacaatt gtcacgataa ccccgacgcc
    58741 tggatctgtc atcggtgttg gagatatcag atgttgaata cccgtcaaaa acaggaatag
    58801 tgctgaaccg gagataaaca tactttgcgc cagcgccgcg agggactcag ctttaccgtg
    58861 accaaacgag tgattatcgt cggcaggttg cagggaatat cgcaccacca gtaaattcgt
    58921 caacgacgcg ccgatatcca ccagcgaatc caccagcgcg gcgagaatac tcaccgaccc
    58981 ggtataccac catgcaaaaa ttttaatcag caatagcagt gaagccatcg ccgttgcagc
    59041 aatcgccgcc cgactgacca gccgtccata agattgattc ataaatactc ccgctatcaa
    59101 ctgacgctag tataacggaa gcaaatcatc tgcaatgcat taagcagcag gcaaattgag
    59161 gataaaaaaa acccccacat catgtggggg aagacaggga tggtgtctat ggcaaggaaa
    59221 acagggttta ctactgggaa cgtgagttgc tactactcaa tagcttcaac gatgaacttt
    59281 tttgccattg cgtcacgtca cgcaactgct ccattcgttg ttgatgtttc tcatttaaaa
    59341 ccgcttgctg ctccggcgtt aacaggcgat acatttggtt gcggactttt gccatctcaa
    59401 cctgacgagc aatttgctca ttcgccattt tttctgcctg tgcgcgcaca gcgttttcat
    59461 caaaattttc tgcggtgaca aggcgatgca ttgtctccag ttcgctaaca ttaacaggag
    59521 gctgttcgtg ccgggcctgt tgcataagat ctcgcatctg ctgacgctga tgttcggtta
    59581 aacttatgcc gtcgaacata tggctctgcg tactgcgctg cgtaagttct tcacccggat
    59641 gccagttatc gcctgaaccg acttcagcag cgtggcttaa tgaactgact gccagcgttg
    59701 aggccatgac ggcagcggta actatgcgca tcatttgctc ccaaaatctt tctgtcgcga
    59761 ttcaacgata gagagtttac gattcaggct gcaaacatgc gtcagggggt gtaaaacaac
    59821 gtaaagtcat ggattagcga cgtctgatga cgtaatttct gcctcggagg tatttaaaca
    59881 atgaataaaa tcctgttagt tgatgatgac cgagagctga cttccctatt aaaggagctg
    59941 ctcgagatgg aaggcttcaa cgtgattgtt gcccacgatg gggaacaggc gcttgatctt
    60001 ctggacgaca gcattgattt acttttgctt gatgtaatga tgccgaagaa aaatggtatc
    60061 gacacattaa aagcacttcg ccagacacac cagacgcctg tcattatgtt gacggcgcgc
    60121 ggcagtgaac ttgatcgcgt tctcggcctt gagctgggcg cagatgacta tctcccgaaa
    60181 ccgtttaatg atcgtgagct ggtggcacgt attcgcgcga tcctgcgccg ttcgcactgg
    60241 agcgaacaac agcaaaacaa cgacaacggt tcaccgacac tggaagttga tgccttaagt
    60301 ctcaatcctg gtcgccagga agccaccttc gacgggcaaa cactggagtt aaccggtact
    60361 gagtttaccc tgctctattt gctggcacag catctgggtc aggtggtttc ccgtgagcat
    60421 ttaagccagg aagtgctggg caagcgcctg acgcctttcg accgcgctat cgatatgcat
    60481 atttccaacc tgcgtcgtaa actgccggat cgtaaggatg gtcacccgtg gtttaaaacc
    60541 ctgcgtggtc gtggctatct gatggtttct gcttcatgat aggcagctta accgcgcgca
    60601 tcttcgccat cttctggctg acgctggcgc tggtgttgat gttggttttg atgttaccca
    60661 agctcgattc acgccagatg accgagcttc tggatagcga acagcgtcag ggtctgatga
    60721 ttgagcagca tgttgaagcg gagctggcga acgatccgcc caacgattta atgtggtggc
    60781 ggcgtctgtt ccgggcgatt gataagtggg caccgccagg acagcgtttg ttattggtga
    60841 ccaccgaagg ccgcgtgatc ggcgctgaac gcagcgaaat gcagatcatt cgtaacttta
    60901 ttggtcaggc cgataacgcc gatcatccgc agaagaaaaa gtatggccgc gtggaactgg
    60961 tcggtccgtt ctccgtgcgt gatggcgaag ataattacca actttatctg attcgtccgg
    61021 ccagcagttc tcaatccgat ttcataaact tactgtttga ccgcccgcta ttactgctga
    61081 ttgtcaccat gctggtcagt acgccgctgc tgttgtggtt ggcctggagt ctggcaaaac
    61141 cggcgcgtaa gctgaaaaac gctgccgatg aagttgccca aggaaactta cgccagcatc
    61201 cggaactgga agcggggcca caggaattcc ttgccgccgg tgccagtttt aaccagatgg
    61261 tcaccgcgct ggagcgcatg atgacctctc agcaacgtct actttctgat atctctcacg
    61321 aactgcgcac cccgctgacg cgtctgcaac tgggtacggc gttactgcgc cgtcgtagcg
    61381 gtgaaagcaa ggaactggag cgtattgaaa ccgaagcgca acgtctggac agcatgatca
    61441 acgatctgtt ggtgatgtca cgtaatcagc agaaaaatgc gctggttagc gagaccatca
    61501 aagccaacca gttgtggagt gaagtgctgg ataacgcggc gttcgaagcc gagcagatgg
    61561 gcaagtcgtt gacggttaac ttcccgcctg ggccgtggcc gctgtacggc aacccgaacg
    61621 ccctggagag tgcgctggaa aacattgttc gtaatgccct gcgttattcc catacgaaga
    61681 ttgaagtggg ctttgcggta gataaagacg gtatcaccat tacggtggac gacgatggtc
    61741 ctggcgttag cccggaagat cgcgaacaga ttttccgtcc gttctatcgt accgatgaag
    61801 cacgcgatcg tgaatctggc ggtacaggtt tggggctggc gattgtagaa accgccattc
    61861 agcagcatcg tggctgggtg aaggcagaag acagcccgct gggcggttta cggctggtga
    61921 tttggttgcc gctgtataag cggagttaaa ctccgcattt gtaggcagga taaggcgttt
    61981 acgccgcatc cggcatttga gcaggatgcc tgatgcgacg ctgatagcgt cttatcaggc
    62041 ctacactccg gggattattt tccccacaat cggcgggaaa aatcttcgat cttgccgctc
    62101 agtcggcgct tttgcatcgt tcgcgtccag cttttcgata cccctgcggc ggagagtaaa
    62161 cggtgatact gatcgtcatc aaacggcata tgccatgcga tggcggcagc ctcctgcacg
    62221 gtcacatcgc tgacacggga aaccaactcc agtggcgcat cagcagatac caaccctggt
    62281 gcaattacgc tatacaacca ccccacttta cccgtgtttt gcatcaactg cgctatatcg
    62341 ctgatatcaa aatggtaatt gagtttatag caaggtgagc gtggctggct gacctgaatt
    62401 aatgcctctc cccagcggaa aatatcgcca atataaacat tactttccgt cagaccgtcg
    62461 gttgagagat tttcgccaaa cgcaggcgca acaaacaact ccgcctgttc cggaaattcc
    62521 cgcgcccagt aaagataatg ctcacgagga taatgacaca gcgctctgtc tggcccgccg
    62581 tgaacctttt tctccgcctg ctcgtcacct tccagcccca ggtccgtcag catcaactca
    62641 ccatcaacct ggattttggc aattgcgctg ggtttgcctt cgggataagc ctgaatcttg
    62701 cctgtgtata catcaaccgg atatcgcatc tgttccctct ccacgagaaa tttccccatt
    62761 agaccatatt tattttatgt caggcgtgct gttttcgcgg taagtatcca ggcaggaact
    62821 caatagcaaa ccggacgaac gttagttcat ccggctgtgg ggattaagga agcaggttta
    62881 tttcaccgta cctaaaattt cgattttcgc tgctctggct ttgactttac gtgaccagat
    62941 agaggtaaga attggcacca gaatcgaagt cacaatgacc gccgtcgcga ccagcgaagt
    63001 tgctgccgga gccatcggtt taaacgcagg caccatttct gcaatcagca caggtgtcgc
    63061 taccgcggcc cctgcggaac tggaagcggc aattccggca gtgccatcgc caccgccaat
    63121 caatttatca gcgataatca acggaatacc ggtcacgata attactgcca cacccaacag
    63181 gatccccagc aaacctgtct gggcaattac cgtcaaatca atggtattac ccagtgcgaa
    63241 ggcaaagaat ggaatcagcg tctgcaccgc tttgctgaaa aattctcgta attcagggtc
    63301 aaggttccca agggcaaagc ccaccaggaa cggtaatact gcgccgacga aaacatgcgg
    63361 ttcaaacgag gcaatcccgg cagtgcccag aataatcatc gtcatgagcg gcccggactc
    63421 caatgacatc aacacaaatg ccccagcttc ttcttttgtg ccgtactgct gcatgatgga
    63481 agcgtaaagt ccgccgttgg tcatatccat cgccgccacc agcgccagcg ttgaaagtcc
    63541 ggcaaagaat ccaacttcaa caccatgttc cggaataatg cgcgaggcaa ttgccgcaac
    63601 cacccacgcg acggcaattt ttgttaccac cagcgtaccg gatttacgca ataccgttcc
    63661 cgtcgcgctt aattttattg acgcccccat gcaaaaaaac cacaccgcca gaatgggcac
    63721 cgtaccggta atcatcccgt tggtgaatga tccaaaatat ttccccgcct ccggcgagaa
    63781 ggtgtggcac agtgcgccaa ggaataacgg gacgagcatc atcccccccg ggattttctc
    63841 aatcgagcgt tttatctgca tttccatcac ctgtcggcat atttttaagg gctgatgcgt
    63901 cattcctcaa tggagcatca gtgttgatgg aaacgaagat agacattcat ttaatcaata
    63961 aaagtgatca aaaccacatc aattgaaaca ctgttttatt tttatcggat cattgttttt
    64021 attttgaaat tgatcacaaa aaaaccaccg ccgttggcga tggttcattt tttaagctga
    64081 tatgcggcgt aaacgcctca ttgcagcagg cggcaaatga ttattttttc gccgcgaaac
    64141 gtgccgctgc ttcgtcccag ttcaccacgt tccagaactc tttgatgtag tcaggacggc
    64201 ggttctggaa tttcaggtag taagcatgtt cccacacatc caggcccaga atcgggaagc
    64261 cggaagcgcc agaaatagct tcacccatca gcggggaatc ctggttagca gtagaaacca
    64321 ccgccagttt atcgcctttc agcaccagcc atgcccagcc ggaaccaaag cgggaagctg
    64381 ccgctttctc aaattctgct ttgaagttat caacggagcc gaagtcacgt tcgatagccg
    64441 ctttcaggtc accctgcagg gtggtgcctt ttttcagacc tttccagaac aggctgtggt
    64501 tagcgtgacc gccagcgttg ttgcgcagta cggttttctt gtctgctggc agctggtcca
    64561 gtttggtgat cagctcttca accggcaggt tggcaaattc tggcaggctt tccagcgccg
    64621 cgttggcgtt gttgacgtag gtctgatggt gtttggtgtg gtggatttcc atggtctgct
    64681 tatcgaagtg cggttccagg gcatcgtaag catacggcag ggatggcagg gtataactca
    64741 tattcatctc cagtgttgtc gggcggccga ttgttaatgc cgcgtaagca gttggttcat
    64801 tatagttaat taaatgatat tgaaaatgat tatcaatgcc gtacttttcg taagggtatg
    64861 gttttgcagg aaaatgcccg agatgtgaag caaatcaccc acttaatgcc gtgattgcca
    64921 gtaaatcgac aacggcggca acaggcgaaa ggttaatcga cagcacgatt tttacactca
    64981 tctcgtcgga gatgtgacgc gacgaaaaat gatgaggata agaagatgag taacgcgatt
    65041 acgatgggga tattttggca tttgatcggc gcggccagtg cagcctgttt ttacgctccg
    65101 ttcaaaaaag taaaaaaatg gtcatgggaa accatgtggt cagttggtgg gattgtttcg
    65161 tggattattc tgccgtgggc catcagcgcc ctgttactac cgaatttctg ggcgtattac
    65221 agctcgttta gtctctctac gctgctgcct gtttttctgt tcggcgcaat gtgggggatt
    65281 ggtaatatca actacggcct gaccatgcgt tatctcggca tgtcgatggg gattggtatc
    65341 gccattggta tcacgttgat tgttggtacg ctgatgacgc caattatcaa cggcaatttc
    65401 gatgtgttga tcaataccga aggtggacgc atgacattgc tcggcgttct ggtggcgctg
    65461 attggtgtag ggattgtgac tcgcgccggg cagttgaaag agcgcaagat gggcattaaa
    65521 gccgaagagt tcaacctgaa aaaagggctg gtgctggcgg tgatgtgcgg cattttctct
    65581 gccggaatgt cctttgcgat gaacgccgca aaaccgatgc atgaagccgc tgccgcactg
    65641 ggcgtcgatc cactgtatgt cgctctgcca agctatgttg tcatcatggg cggcggcgcg
    65701 atcattaacc tcggtttctg cttcattcgt ctggcaaaag tgaaggattt gtcgctaaaa
    65761 gccgacttct cgctggcaaa accgctgatc attcacaatg tgttactctc agcactgggc
    65821 gggttgatgt ggtatctgca attctttttc tatgcctggg gccacgcccg cattccggcg
    65881 cagtatgact acatcagttg gatgctgcat atgagcttct atgtattgtg cggcggtatc
    65941 gtcgggttgg tgctgaaaga gtggaacaat gcaggtcgcc gtccggtaac ggtgttgagc
    66001 ctcggttgtg tggtgattat tgtcgccgcc aacatcgtcg gcatgggcat ggcgaattaa
    66061 tctttctgcg aattgagatg acgccactgg ctgggcgtca tcccggtttc ccgggtaaac
    66121 accaccgaaa aatagttact atcttcaaag ccacattcgg ttgaaatatc actgattaac
    66181 aggcggctat gctggagaag atattgcgca tgacacactc tgacctgccg cagatattga
    66241 ttgatggtca ttccagtctg ctggcgaaat tgctgacgca aaacgcgctc actgcacgat
    66301 gcctcatcac aaaatttatc cagcgcaaag ggacttttca ggctggccgc cagccgggta
    66361 atcagcttat ccagcaacgt ttcgctggat gttggcggca acgaatcact ggtgtaacga
    66421 tggcgattca gcaacatcac caactgcccg aacagcaact ctgccatttc gttagcaaac
    66481 ggcacatgct gactactttc atgctcaagc tgaccgataa tctgccgcgc ctgcgccatc
    66541 cccacgctac ctaagcgcca gtgtggttgc cctgcgctgg cactaaatcc cggaatcgcc
    66601 ccctgccagt caagattcag cttcagccgc tccgggcaat aaataatatt ctgcaaaacc
    66661 agatcgttaa cggaagcgta ggagtgttta tcgtcagcat gaatgtaaaa gagatcgcca
    66721 cgggtaatgc gataagggcg atcgttgagt acatgcagac cattaccgcg ccagacaatt
    66781 accagctcac aaaaatcatg tgtatgttca gcaaagacat cttgcggata acggtcagcc
    66841 acagcgactg cctgctggtc gctggcaaaa aaatcatctt tgagaagttt taactgatgc
    66901 gccaccgtgg ctacctcggc cagagaacga agttgattat tcgcaatatg gcgtacaaat
    66961 acgttgagaa gattcgcgtt attgcagaaa accatcccgt ccctgacgaa tatcacgcgg
    67021 tgaccagtta aactctcggc gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccgca
    67081 gcgataggcg atgtcagtaa cgctggcctc gctgtggcgt agcagatatc gggctttcat
    67141 cagtcgcagg cggttcaggt aacgctgagg cgtcagcccc gtttgctgct taagctggcg
    67201 atgcagcgta cgcaatgaaa gagaaaattg atccgccacg gcatcccaat tcacctcatc
    67261 ggcaaaatgg tcctccagcc aggccagaag caagttgaga cgtgacgcgc tgttttccag
    67321 gttctcctgc aaactgcttt tacgcagcaa gagcagtaat tgcataaata agatctcgcg
    67381 actggcggtc gagggtaaat cattttcctc ttcctgctgt tccatctgtg caaccagctg
    67441 tcgcacctgc tgcaacacgc tgtggttaac gcgccagtga gacggatact gcccatcctg
    67501 ctcttgtggc agcaactgat tcagcccggc gagaaactga aatcgatccg gcgagcgata
    67561 cagcacattg gtcagacaca gattatcggt atgttcatac agatgccgat catgatcgcg
    67621 tacgaaacag accgtgccac cggtgatggt atagggctgc ccattaaaca catgaatacc
    67681 cgtgccatgt tcgacaatca caatttcatg aaaatcatga tgatgttcag gaaaatccgc
    67741 ctgcgggagc cggggttcta tcgccacgga cgcgttacca gacggaaaaa aatccacact
    67801 atgtaatacg gtcatactgg cctcctgatg tcgtcaacac ggcgaaatag taatcacgag
    67861 gtcaggttct taccttaaat tttcgacggg aaaccacgta aaaaacgtcg atttttcaag
    67921 atacagcgtg aattttcagg aaatgcggtg agcatcacac caccacaatt cagcgaattg
    67981 tgaacatcat cacgttcatc tttccctggt tgccaatgac ccattttcct gtcagtaacg
    68041 agaaggtcgc gaattcaggc gctttttaga ctggtcgtaa tgaaattcag caggatcaca
    68101 ttatgacctt tcgcaattgt gtcgccgtcg atctcggcgc atccagtgga cgcgtgatgc
    68161 tggcgcgtta cgagcgtgaa tgccgcagcc tgacgctgcg cgaaatccat cgttttaaca
    68221 atgggctgca tagccagaac ggttatgtca cctgggatgt ggatagcctg gaaagtgcca
    68281 ttcgccttgg attaaacaag gtgtgcgagg aagggattcg tatcgatagc attgggattg
    68341 atacctgggg cgtggacttt gtgctgcttg accaacaggg tcagcgcgtg ggcctgcctg
    68401 ttgcttatcg cgatagccgc acccatggtc tgatggcgca ggcacaacgg caactcggca
    68461 aatgcgatat ttatcaacgt agcggcatcc agtttttgcc cttcaatacg ctttatcagt
    68521 tgcgtgcgct gacggagcaa caacctgaac ttattccaca cattgctcac gctctgctga
    68581 tgccggatta cttcagttat cgtctgaccg gcaagatgaa ctgggaatat accaacgcca
    68641 cgaccacgca actggtcaat atcaatagcg acgactggga cgagtcgcta ctggcgtgga
    68701 gcggggccaa caaagcctgg tttggtcgcc cgacgcatcc gggtaatgtc attgggcact
    68761 ggatttgccc gcagagtaat gagattccgg tggtcgccgt tgccagccat gataccgcca
    68821 gcgcggttat cgcctcgccg ttaaacagtt cacgcgccgc ttatctctct tctggcacct
    68881 ggtcattgat gggcttcgaa agccagacgc catttaccaa tgacacggcg ctggcagcca
    68941 acatcaccaa tgaaggcggg gcggaaggtc gctatcgggt gctgaaaaat attatgggct
    69001 tatggctgct tcagcgggtg cttcaggagc ggcaaatcaa cgatcttccg gcgcttatcg
    69061 ccgcgacaca ggcacttccg gcttgccgct tcaccatcaa tcccaatgac gatcgcttta
    69121 ttaatcctga cgagatgtgc agcgaaattc aggctgcgtg tcgggaaacg gcgcaaccaa
    69181 tcccggaaag tgatgctgaa ctggcgcgct gtattttcga cagtctggcg ctgctgtatg
    69241 ccgatgtgtt gcatgagctg gcgcaactgc gcggtgaaga tttctcacaa ctgcatattg
    69301 tcggcggtgg ctgccagaac gcgctgctca accagttatg tgctgatgcc tgcggtattc
    69361 gggtgatcgc cgggcctgtt gaagcctcga cgctcggcaa tatcggcatc cagttaatga
    69421 cgctggatga actcaacaat gtggatgatt tccgtcaggt cgtcagcacc accgcgaatc
    69481 tgaccacctt tacccctaat cctgacagtg aaattgccca ctatgtggcg cagattcact
    69541 ctacacgaca gacaaaggag ctttgcgcat gaccactcaa ctggaacagg cctgggagct
    69601 ggcgaaacag cgtttcgcgg cagtagggat tgatgtcgag gaggcgctgc gccaacttga
    69661 tcgtttaccc gtttcaatgc actgctggca gggcgatgat gtttccggtt ttgaaaaccc
    69721 ggaaggttcg ctgaccggtg ggattcaggc tactggtaat tatccgggca aagcgcgtaa
    69781 tgccagcgag ctacgtgccg atctggaaca agctatgcgg ctgattccgg ggccgaaacg
    69841 gcttaattta catgccatct atctggaatc agatacgcca gtcgcgcgcg accaaatcaa
    69901 accagagcac ttcaaaaact gggttgaatg ggcgaaaacc aatcagctcg gtctggattt
    69961 taacccctcc tgtttttcgc atccgctaag cgccgatggc tttacgcttt cccatgccga
    70021 cgacagtatt cgccagttct ggattgatca ctgcaaggcc agccgccgtg tttcagccta
    70081 ttttggcgag caactcggca caccgtcggt gatgaacatc tggatcccgg atggcatgaa
    70141 agatatcacc gttgaccgtc tcgccccgcg ccagcgtctg ctggcagcac tggatgaggt
    70201 gatcagcgag aagctggatc cggcgcatca tatcgatgcc gttgagagca aattgtttgg
    70261 cattggcgcg gagagctaca cggttggctc caatgagttt tacctggggt atgccaccag
    70321 ccgccagacg gcgctgtgcc tggacgccgg gcatttccac ccgactgaag tgatttccga
    70381 caagatttcc gccgccatgc tgtatgtgcc gcagttgctg ctgcacgtca gccgtccggt
    70441 tcgctgggac agcgatcacg tagtgctgct ggatgatgaa acccaggcga ttgccagtga
    70501 gattgttcgt cacgatctgt tcgaccgggt gcatatcggc ctcgacttct tcgatgcctc
    70561 tatcaaccgc attgccgcgt gggtcattgg tacacgcaat atgaaaaaag ccctgctacg
    70621 tgcgttactg gaacctaccg ctgaactacg caagctggaa tcggcgggcg attacactgc
    70681 gcgtctggcg ctactggaag agcagaaatc gttgccgtgg caggcggtat gggaaatgta
    70741 ttgtcagcgt cacgatacgc cagcgggtag cgaatggctg gagaacgtgc gggcttatga
    70801 gaaagaaatt ttgagtcgcc gcgggtaaac actgccggat gcggcacaag tgccttatcc
    70861 ggcctacggg tcggcaatag ttgtaggcct gataagacgc gacagcgtcg catcaggcat
    70921 tgattgccgg atgcggcacg agtgccttat ccggcctaca gatcgtatat ccggcaacag
    70981 gcacggacgt ccaattaaca ggaacaccga acatgcaaaa cattactcag tcctggtttg
    71041 tccagggaat gattaaagcc accaccgacg cctggctgaa aggctgggac gagcgcaacg
    71101 gcggcaacct gacgctgcgc ctggatgacg ccgatatcgc accatatcac gacaattttc
    71161 accaacaacc gcgctatatc ccgctcagcc agcccatgcc tttactggca aatacaccgt
    71221 ttattgtcac cggctcaggc aaattcttcc gtaacgtcca gcttgatcct gcggctaact
    71281 taggcatcgt aaaagtcgac agcgacggcg cgggctacca cattctttgg gggttaacca
    71341 acaaagccgt ccccacttcc gaacttccgg ctcactttct ctcccactgc gagcgcatta
    71401 aagccaccaa cggtaaagat cgggtgatca tgcactgcca tgccaccaac ctgatcgccc
    71461 ttacctacgt actggaaaac gacaccgcgg tcttcactcg ccaactgtgg gaaggcagca
    71521 ccgaatgtct ggtagtgttc ccggatggcg tcggcatttt gccgtggatg gtgcccggca
    71581 ccgacgaaat cggccaggcg accgcacagg aaatgcaaaa acattcgctg gtgttgtggc
    71641 ccttccacgg cgtcttcggc agcggtccga cactggatga aacattcggt ttaatcgaca
    71701 ccgcagaaaa atcagcacaa gtattagtga aggtttattc gatgggcggc atgaaacaga
    71761 ccatcagtcg tgaagagctg atagcgctcg gccagcgttt cggcgttaca ccactcgcca
    71821 gtgcgctggc gctgtaagga gcaaacatga tccgcaaagc ctttgtcatg caggttaacc
    71881 ccgacgccca cgaagagtat cagcgtcgtc ataatccaat ctggccggaa ctggaagcag
    71941 tgttgaaatc tcacggtgcg cataactacg ccatttacct cgacaaagcg cgtaatctgc
    72001 tgtttgccac ggtagagatt gaatctgaag aacggtggaa tgcggtagcc agtactgatg
    72061 tttgccaacg ttggtggaaa tatatgaccg acgttatgcc aactaacccg gataacagcc
    72121 cagtcagtag cgagctgcaa gaagtgtttt acctgcctta atgagttgcg gtgccggaaa
    72181 atatccagca ccgcgacttc atcagaagaa gtatttaaag cgaacgcgtg caccgtaacg
    72241 gtcatcagta tcatcaccgt gttttttatc accgtcgcta tccagaatgg agtaatacgt
    72301 cccgaggtaa atattaaagt tctccatatc catcacatta gcgaactgat aagacgcatg
    72361 aatggtgtga atggtgtacg tcccttcatc atcaatccag cagtcgttat cacaaactac
    72421 accgctgaat tcatcaatct tattgtgcgc gtagatataa cccagctcga aacgtttcca
    72481 cagcgcgtta atcccggcag tgaaatcttt ctcattatta gcatctaaat aggcggtatt
    72541 aagattaacc acgacaccat tttccggatc cgttttcagg ccattccagg tcatgctcat
    72601 accatagccg gtacgatcgg actgatcgac aaaattaccc ttgctatcgg tataaccata
    72661 agcattattt accacgttcg cttccatcgc tgcggaaacg gtgaattctt ccgtcggcga
    72721 ccaggcaatt accggacgca gataagcaac atttttctgc tgttccatat cgcgtccatg
    72781 ataattaccg tcgttatata aagatgttcc gtcttccagt aacgtgttta attcaaaata
    72841 ccagttatcg agttgtttgc tgacgaggaa attaccgcca gcgttagaac gcccgcggcc
    72901 ctctttcatc atatagatat aaccgctgcc atcgtcataa agatcgttcg cagtattacc
    72961 ggaatgttca acaaaggtat cctgattcag cgggaacata tcgtaggctt caaaacggcc
    73021 tactttgacc ttccagtcgt tttctttgcc aaagaagaat accgcatcat cgatatttac
    73081 tgaaccgtgc atgtccccca gcggttgtgc ggagaacccg gcgaaataac cgttgtccat
    73141 ctttcgcata ccgtcaaaac ctaacagaat acgaccattg agattccact gttcattagt
    73201 gggatcgcta ttcacatcag cgttggtcat tgccagaagg ccatgattac tttccgcatc
    73261 catattaaat tctacgtcgc cgtagatttt taaatcgcca taacctgaaa gcaaaagatt
    73321 aggtgaggtg gaatttttct ttgcagattc cgacgttgcc tgtccattta ccggcgtatt
    73381 gccctgggca gccttaattt cgcggatctc agcggcttgt tgtgtctgca gctgtttaac
    73441 ctggctctct gcttttgctg cccgcatttc tgccgcttcc aggcgttctt ccagcaatgc
    73501 catgcgttgt tccagcgtta aatgctgtgt ttttgccatt ccggtactac atgccaataa
    73561 taaaccgatg gctaaagata atgtcgattt tttcattatt tacgttccct gaaataaaag
    73621 agcccctacg ctttccaaaa ggaatacgca tatagagaaa tataaggtgt gcactttcga
    73681 atttttaatc caacttcagc aggccgatgg atagtatccc gccatataat atgagtaaat
    73741 ccgctgattt cctggaacgt gaggaatata acaatagtgc cgtttttaac taaatatcgt
    73801 aaaaaatatt agatatgatt cacgctgact cataagccac aaatataaat aac
//
LOCUS       DAZKGF010000005        63228 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZKGF010000005 DAZKGF010000000
VERSION     DAZKGF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.005"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(201..277)
                     /locus_tag="ACHOQV_000312"
     tRNA            complement(201..277)
                     /locus_tag="ACHOQV_000312"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(241..243),aa:Arg,seq:acg)
     gene            complement(281..373)
                     /locus_tag="ACHOQV_000313"
     tRNA            complement(281..373)
                     /locus_tag="ACHOQV_000313"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(337..339),aa:Ser,seq:gct)
     gene            complement(689..874)
                     /gene="csrA"
                     /locus_tag="ACHOQV_000314"
     CDS             complement(689..874)
                     /gene="csrA"
                     /locus_tag="ACHOQV_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFP9628744.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1109..3739)
                     /gene="alaS"
                     /locus_tag="ACHOQV_000315"
     CDS             complement(1109..3739)
                     /gene="alaS"
                     /locus_tag="ACHOQV_000315"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP9628745.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVNSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLNEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3867..4367)
                     /gene="recX"
                     /locus_tag="ACHOQV_000316"
     CDS             complement(3867..4367)
                     /gene="recX"
                     /locus_tag="ACHOQV_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP9628746.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4436..5497)
                     /gene="recA"
                     /locus_tag="ACHOQV_000317"
     CDS             complement(4436..5497)
                     /gene="recA"
                     /locus_tag="ACHOQV_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP9628747.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5577..6074)
                     /gene="pncC"
                     /locus_tag="ACHOQV_000318"
     CDS             complement(5577..6074)
                     /gene="pncC"
                     /locus_tag="ACHOQV_000318"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFP9628748.1"
                     /translation="MTDSELMQLSEQVGQSLKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6219..7304)
                     /gene="mltB"
                     /locus_tag="ACHOQV_000319"
     CDS             complement(6219..7304)
                     /gene="mltB"
                     /locus_tag="ACHOQV_000319"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFP9628749.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7560..8123
                     /gene="srlA"
                     /locus_tag="ACHOQV_000320"
                     /gene_synonym="gutA"
     CDS             7560..8123
                     /gene="srlA"
                     /locus_tag="ACHOQV_000320"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP9628750.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8120..9079
                     /gene="srlE"
                     /locus_tag="ACHOQV_000321"
                     /gene_synonym="gutE"
     CDS             8120..9079
                     /gene="srlE"
                     /locus_tag="ACHOQV_000321"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP9628751.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9090..9461
                     /gene="srlB"
                     /locus_tag="ACHOQV_000322"
                     /gene_synonym="gutB"
     CDS             9090..9461
                     /gene="srlB"
                     /locus_tag="ACHOQV_000322"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9628752.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9465..10244
                     /gene="srlD"
                     /locus_tag="ACHOQV_000323"
     CDS             9465..10244
                     /gene="srlD"
                     /locus_tag="ACHOQV_000323"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFP9628753.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10350..10709
                     /gene="gutM"
                     /locus_tag="ACHOQV_000324"
     CDS             10350..10709
                     /gene="gutM"
                     /locus_tag="ACHOQV_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP9628754.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMYAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10776..11549
                     /gene="srlR"
                     /locus_tag="ACHOQV_000325"
                     /gene_synonym="gutR"
     CDS             10776..11549
                     /gene="srlR"
                     /locus_tag="ACHOQV_000325"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFP9628755.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEKKGIDVIITGESNE"
     gene            11542..12507
                     /gene="gutQ"
                     /locus_tag="ACHOQV_000326"
     CDS             11542..12507
                     /gene="gutQ"
                     /locus_tag="ACHOQV_000326"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFP9628756.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12504..14018)
                     /gene="norR"
                     /locus_tag="ACHOQV_000327"
     CDS             complement(12504..14018)
                     /gene="norR"
                     /locus_tag="ACHOQV_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFP9628757.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     IVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14205..15644
                     /gene="norV"
                     /locus_tag="ACHOQV_000328"
     CDS             14205..15644
                     /gene="norV"
                     /locus_tag="ACHOQV_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFP9628758.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLTDIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15641..16774
                     /gene="norW"
                     /locus_tag="ACHOQV_000329"
     CDS             15641..16774
                     /gene="norW"
                     /locus_tag="ACHOQV_000329"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFP9628759.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDACIPLTLIAADSMDEYNKP
                     DLSHVISQRQRADDLTRQTAGEFAEQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRNARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILAALMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(16998..19250)
                     /gene="hypF"
                     /locus_tag="ACHOQV_000330"
     CDS             complement(16998..19250)
                     /gene="hypF"
                     /locus_tag="ACHOQV_000330"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFP9628760.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAGLGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPLVDNQLDLATFWQQWLSWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19403..19930)
                     /gene="hydN"
                     /locus_tag="ACHOQV_000331"
     CDS             complement(19403..19930)
                     /gene="hydN"
                     /locus_tag="ACHOQV_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFP9628761.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20079..21089)
                     /gene="ascG"
                     /locus_tag="ACHOQV_000332"
     CDS             complement(20079..21089)
                     /gene="ascG"
                     /locus_tag="ACHOQV_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFP9628762.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21349..22806
                     /gene="ascF"
                     /locus_tag="ACHOQV_000333"
     CDS             21349..22806
                     /gene="ascF"
                     /locus_tag="ACHOQV_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFP9628763.1"
                     /translation="MDKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSAQPTVAKEVSLN"
     gene            22815..24239
                     /gene="ascB"
                     /locus_tag="ACHOQV_000334"
     CDS             22815..24239
                     /gene="ascB"
                     /locus_tag="ACHOQV_000334"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFP9628764.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LARTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24397..24867)
                     /gene="hycI"
                     /locus_tag="ACHOQV_000335"
     CDS             complement(24397..24867)
                     /gene="hycI"
                     /locus_tag="ACHOQV_000335"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFP9628765.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(24860..25270)
                     /gene="hycH"
                     /locus_tag="ACHOQV_000336"
     CDS             complement(24860..25270)
                     /gene="hycH"
                     /locus_tag="ACHOQV_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFP9628766.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(25267..26034)
                     /gene="hycG"
                     /locus_tag="ACHOQV_000337"
     CDS             complement(25267..26034)
                     /gene="hycG"
                     /locus_tag="ACHOQV_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFP9628767.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(26034..26576)
                     /gene="hycF"
                     /locus_tag="ACHOQV_000338"
     CDS             complement(26034..26576)
                     /gene="hycF"
                     /locus_tag="ACHOQV_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFP9628768.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(26586..28295)
                     /gene="hycE"
                     /locus_tag="ACHOQV_000339"
     CDS             complement(26586..28295)
                     /gene="hycE"
                     /locus_tag="ACHOQV_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFP9628769.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTDARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(28313..29236)
                     /gene="hycD"
                     /locus_tag="ACHOQV_000340"
     CDS             complement(28313..29236)
                     /gene="hycD"
                     /locus_tag="ACHOQV_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFP9628770.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(29239..31065)
                     /gene="hycC"
                     /locus_tag="ACHOQV_000341"
     CDS             complement(29239..31065)
                     /gene="hycC"
                     /locus_tag="ACHOQV_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFP9628771.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(31062..31673)
                     /gene="hycB"
                     /locus_tag="ACHOQV_000342"
     CDS             complement(31062..31673)
                     /gene="hycB"
                     /locus_tag="ACHOQV_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFP9628772.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(31798..32259)
                     /gene="hycA"
                     /locus_tag="ACHOQV_000343"
     CDS             complement(31798..32259)
                     /gene="hycA"
                     /locus_tag="ACHOQV_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFP9628773.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            32471..32821
                     /gene="hypA"
                     /locus_tag="ACHOQV_000344"
     CDS             32471..32821
                     /gene="hypA"
                     /locus_tag="ACHOQV_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP9628774.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            32825..33697
                     /gene="hypB"
                     /locus_tag="ACHOQV_000345"
     CDS             32825..33697
                     /gene="hypB"
                     /locus_tag="ACHOQV_000345"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFP9628775.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            33688..33960
                     /gene="hypC"
                     /locus_tag="ACHOQV_000346"
     CDS             33688..33960
                     /gene="hypC"
                     /locus_tag="ACHOQV_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFP9628776.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            33960..35081
                     /gene="hypD"
                     /locus_tag="ACHOQV_000347"
     CDS             33960..35081
                     /gene="hypD"
                     /locus_tag="ACHOQV_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFP9628777.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAETHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            35078..36088
                     /gene="hypE"
                     /locus_tag="ACHOQV_000348"
     CDS             35078..36088
                     /gene="hypE"
                     /locus_tag="ACHOQV_000348"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFP9628778.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            36162..38240
                     /gene="flhA"
                     /locus_tag="ACHOQV_000349"
     CDS             36162..38240
                     /gene="flhA"
                     /locus_tag="ACHOQV_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFP9628779.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRHDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLSLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            38285..38713
                     /locus_tag="ACHOQV_000350"
     CDS             38285..38713
                     /locus_tag="ACHOQV_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFP9628780.1"
                     /translation="MAVSARNQLTGTVSTVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(38752..39096)
                     /gene="ygbA"
                     /locus_tag="ACHOQV_000351"
     CDS             complement(38752..39096)
                     /gene="ygbA"
                     /locus_tag="ACHOQV_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFP9628781.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            39382..41943
                     /gene="mutS"
                     /locus_tag="ACHOQV_000352"
     CDS             39382..41943
                     /gene="mutS"
                     /locus_tag="ACHOQV_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9628782.1"
                     /translation="MRTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLEGVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIATGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            42049..42705
                     /gene="pphB"
                     /locus_tag="ACHOQV_000353"
     CDS             42049..42705
                     /gene="pphB"
                     /locus_tag="ACHOQV_000353"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP9628783.1"
                     /translation="MPSTRYQKINAHHYHHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(42756..43523)
                     /gene="ygbI"
                     /locus_tag="ACHOQV_000354"
     CDS             complement(42756..43523)
                     /gene="ygbI"
                     /locus_tag="ACHOQV_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFP9628784.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLTHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            43719..44627
                     /gene="ltnD"
                     /locus_tag="ACHOQV_000355"
     CDS             43719..44627
                     /gene="ltnD"
                     /locus_tag="ACHOQV_000355"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFP9628785.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            44624..45886
                     /gene="otnK"
                     /locus_tag="ACHOQV_000356"
     CDS             44624..45886
                     /gene="otnK"
                     /locus_tag="ACHOQV_000356"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFP9628786.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSSAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDTPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            45883..46521
                     /gene="ygbL"
                     /locus_tag="ACHOQV_000357"
     CDS             45883..46521
                     /gene="ygbL"
                     /locus_tag="ACHOQV_000357"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFP9628787.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            46526..47302
                     /gene="ygbM"
                     /locus_tag="ACHOQV_000358"
     CDS             46526..47302
                     /gene="ygbM"
                     /locus_tag="ACHOQV_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFP9628788.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLAQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADHFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            47391..48755
                     /gene="ygbN"
                     /locus_tag="ACHOQV_000359"
     CDS             47391..48755
                     /gene="ygbN"
                     /locus_tag="ACHOQV_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFP9628789.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCI
                     WAVI"
     gene            complement(48793..49029)
                     /locus_tag="ACHOQV_000360"
     CDS             complement(48793..49029)
                     /locus_tag="ACHOQV_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFP9628790.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWMVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(49040..50467)
                     /locus_tag="ACHOQV_000361"
     CDS             complement(49040..50467)
                     /locus_tag="ACHOQV_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFP9628791.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFIDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFNILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARQ"
     gene            complement(50467..51060)
                     /locus_tag="ACHOQV_000362"
     CDS             complement(50467..51060)
                     /locus_tag="ACHOQV_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFP9628792.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            51207..51614
                     /locus_tag="ACHOQV_000363"
     CDS             51207..51614
                     /locus_tag="ACHOQV_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9628793.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSAEEQELFMQLVRKMMSK"
     gene            complement(51735..52727)
                     /gene="rpoS"
                     /locus_tag="ACHOQV_000364"
     CDS             complement(51735..52727)
                     /gene="rpoS"
                     /locus_tag="ACHOQV_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFP9628794.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(52790..53884)
                     /gene="nlpD"
                     /locus_tag="ACHOQV_000365"
     CDS             complement(52790..53884)
                     /gene="nlpD"
                     /locus_tag="ACHOQV_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFP9628795.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(54069..54695)
                     /gene="pcm"
                     /locus_tag="ACHOQV_000366"
     CDS             complement(54069..54695)
                     /gene="pcm"
                     /locus_tag="ACHOQV_000366"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFP9628796.1"
                     /translation="MVSRRVQALLDQLRAQGIQDELVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(54689..55450)
                     /gene="surE"
                     /locus_tag="ACHOQV_000367"
     CDS             complement(54689..55450)
                     /gene="surE"
                     /locus_tag="ACHOQV_000367"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFP9628797.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(55431..56480)
                     /gene="truD"
                     /locus_tag="ACHOQV_000368"
     CDS             complement(55431..56480)
                     /gene="truD"
                     /locus_tag="ACHOQV_000368"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFP9628798.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQKLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(56477..56956)
                     /gene="ispF"
                     /locus_tag="ACHOQV_000369"
     CDS             complement(56477..56956)
                     /gene="ispF"
                     /locus_tag="ACHOQV_000369"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFP9628799.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(56956..57666)
                     /gene="ispD"
                     /locus_tag="ACHOQV_000370"
     CDS             complement(56956..57666)
                     /gene="ispD"
                     /locus_tag="ACHOQV_000370"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFP9628800.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(57685..57996)
                     /gene="ftsB"
                     /locus_tag="ACHOQV_000371"
     CDS             complement(57685..57996)
                     /gene="ftsB"
                     /locus_tag="ACHOQV_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFP9628801.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(58189..58512)
                     /gene="ygbE"
                     /locus_tag="ACHOQV_000372"
     CDS             complement(58189..58512)
                     /gene="ygbE"
                     /locus_tag="ACHOQV_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFP9628802.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(58562..59167)
                     /gene="cysC"
                     /locus_tag="ACHOQV_000373"
     CDS             complement(58562..59167)
                     /gene="cysC"
                     /locus_tag="ACHOQV_000373"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFP9628803.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(59167..60594)
                     /gene="cysN"
                     /locus_tag="ACHOQV_000374"
     CDS             complement(59167..60594)
                     /gene="cysN"
                     /locus_tag="ACHOQV_000374"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFP9628804.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(60596..61504)
                     /gene="cysD"
                     /locus_tag="ACHOQV_000375"
     CDS             complement(60596..61504)
                     /gene="cysD"
                     /locus_tag="ACHOQV_000375"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFP9628805.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            61756..62793
                     /gene="iap"
                     /locus_tag="ACHOQV_000376"
     CDS             61756..62793
                     /gene="iap"
                     /locus_tag="ACHOQV_000376"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFP9628806.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   62875..63206
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=62936..62962
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaac"
BASE COUNT        14865 a        17269 c        16457 g        14637 t
ORIGIN      
        1 gcatcgggaa acttacttta ctgcagattt tttgataccg ctactaaagc cgtatcaagt
       61 aagagatggt gcatccggga ggattactcg ccttgcggct cgcccttcgg gccgttgcta
      121 aagcaacgtt atcctccctg gtgcctgcgg ttattaccgc cacctcttcc ataccttcat
      181 cgctgaaggt acgtagaata tggtgcatcc gggaggattc gaacctccga ccgctcggtt
      241 cgtagccgag tactctatcc agctgagcta cggatgcaaa tggcggtgag gcggggattc
      301 gaaccccgga tgcagctttt gaccgcatac tcccttagca ggggagcgcc ttcagcctct
      361 cggccacctc accacacgcc tcttacgagt gcttcgaaga acttgtttat cgctcatcgt
      421 cgctgcgtgg cgcacatatt actttctgag acttataagt caaacaattt ttcccacact
      481 tttatcgttt gcacacttca cgctcaatta gtctgtaaaa acgtcaaaaa gagtgtttta
      541 tcaacagaaa aacggaggtc tgtcagatag cagtaatgca aaaaaatgga gacttaagtt
      601 gaatgaacgg gagtaaagcg aaaagacaat ggagtgaaga agaaattttg agggtgcgtc
      661 tcaccgataa agatgagacg cggaaagatt agtaactgga ctgctgggat ttttcagcct
      721 ggatacgctg gtagatctct tcacggtgaa cagaaacttc cttcggggca tttacgccaa
      781 tacgtacctg gttgcccttt acccctaaaa ctgtcacggt gacctcatcc ccaatcatga
      841 gggtctcacc aactcgacga gtcagaatca gcattctttg ctccttgaaa gattaaaaga
      901 gtcgggtctc tctgtatccc ggcattatcc atcatataac gccaaaaagt aagcaatgac
      961 aaacacatta catctaagca gtcatggcat tacattctgt taaacctaag tttagccgat
     1021 atacacaact tcaacctgac tttatcgttg tcgatagcgt taatgcgaat gccgtgaagc
     1081 gagtccacgg cattgcctga cgcttatatt attgcaattt cgcgctgacc cagcctttca
     1141 cactggctaa cgctgcaggt aaggccgcag catccgtacc accggcttgc gccatgtcag
     1201 gacgtccacc acccttgccg cccacctgct gagcgaccat accaatcagt tcccctgctt
     1261 tcacacgatc tgtgacgtcc ttagatacgc ctgcaatcag agaaacctta ccttcggcta
     1321 ccgttgccag cacgataatt gtcgacccca gctgattttt taaatcgtca accatggtac
     1381 gcaacatttt cggctcaaca ccgctaagct cgctaaccaa cagcttaaca ccattaacat
     1441 caattgcctt actggaaaga tttgcgctct cctgtgcggc agcttgttct ttaagctgtt
     1501 gtaactcttt ttccagctga cgcgtacgtt ccagtactga gcgcacttta tcagccagat
     1561 tattgctatc gcctttcagc agatgcgcga cttcgtttaa gcgatcgctg tctgcatgaa
     1621 cggtggcgat agcgccttct ccggttaccg cttcgatacg acgaacgcct gcagcagtac
     1681 ccgattcaga gatgatgcgg aacagaccaa tatcaccagt gcgactggcg tgagtaccgc
     1741 cacacagctc ggtggagaaa tcgcccatgc tcagcacacg tacacgttca tcatacttct
     1801 cgccgaacag cgccatcgca cctttcgctt tcgccgcttc gagatccatg atgttggttt
     1861 caatcggcaa gttacggcga atctgtgcgt tcaccaggtc ttcgaccgca cgaatctctt
     1921 ctggtttcat cgcttcgttg tgtgagaagt cgaagcgcag cactttgtcg ttaaccagtg
     1981 aacctttctg cgatacatga gtacccagaa cctggcgcag cgcagcgtgc atcaggtgcg
     2041 ttgcggagtg attcagacga atacgggcgc gacgagcttc atcaacatca gcctgcaccg
     2101 cgtcgcccac tttcagagaa cccgcagcaa gtttaccgat gtgaccaatc gcctggccgt
     2161 atttctgcgt atcttccacc gcaaaggaga agttagcgcc tttcagttcg cctttatcac
     2221 caacctgacc gccggattcc gcatagaatg gcgtttgatc cagcacgacc acagcttcct
     2281 ggcctgcatt gatggcatca accgctttac catcaacaaa cagcgcggtc actttgccgt
     2341 tcagttccag atggtcatag cctttaaatt cagatgcact gttaacacgg atcattgcgt
     2401 tgtaatcggc accaaagccg ctggcttcgc gcgcacgacg acgctgctct tccattgctg
     2461 cttcaaatcc agcttcgtca actttgatgt tgcgctcacg acaaacatca gccgtcaggt
     2521 caaccgggaa gccataggtg tcgtacagac ggaaagccgt ttcaccatcc agcgtatcac
     2581 cagaaagttt tgccagctct tcgtccagca gcgccagacc gcgctccaga gtacgggcaa
     2641 actgctcttc ttcagtcttc agcacctgct caacctgcgc ctgctggcgt ttcaggtctt
     2701 cacccgcaga gcccattacg tcgatcagcg gaccaaccag tttgtagaag aaggtttctt
     2761 tcgcgcccag catattgccg tgacgcactg cgcgacgaat gatacgacgc agtacataac
     2821 cacggttttc gttggacggc attacgccat ccgcgatcag gaacgcacaa gaacgaatgt
     2881 ggtcagcgat tacgcgcagc gatttgttgc tcaaatcggt cgcgccagtg actttcgcta
     2941 ccgcctggat cagcgtacgg aacaggtcga tgtcatagtt agagttaacg tgttgcagca
     3001 ccgcagcaat acgctccaga cccataccgg tatctacaga cggcttcggc agcggttcca
     3061 tcgtgccatc tgcctggcgg ttgaactgca tgaagacgat gttccagatc tcaatgtagc
     3121 ggtcgccgtc ttcttccgga cttcccggag ggccacccca aatgtggtcg ccgtgatcgt
     3181 agaagatttc ggtgcacggg ccgcacggac cagtgtcacc catctgccag aagttgtcag
     3241 atgcgtatgg cgcacctttg ttatcgccga tgcgaataat acgttcgcgc gggatcccga
     3301 cttctttttc ccagatttcg taggcttcgt cgtcgctttc atagacggta acccacagac
     3361 gctctttcgg cagggcaaac catttttcgc tggtcagcag ttcccatgca aactgaatgg
     3421 catcgtgttt gaaatagtcg ccgaagctga agttgcccag catttcgaag aaggtatggt
     3481 gacgcgcggt gtaaccgacg ttttccaggt cgttgtgttt accacccgca cgcacgcagc
     3541 gttgggaagt ggtagcgcgg gaataattac gcttgtcgag cccaaggaac acatccttga
     3601 actggttcat cccggcgttg gtaaacaaca aagttgggtc gttatggggt accagggagc
     3661 tgctggcaac tacctgatgt cccttactat ggaaaaagtc gagaaacgcc tgacggatct
     3721 cagcggtgct cttgctcata attatcctga aatcaagcta acgaaatatc gccaccagct
     3781 acagcgtgtt ttaaccgccg ggctggtaac tgaaaagtgg gaataagata agttttcttg
     3841 actgggaagt aaaatcccgt atgcgttcag tcggcaaaat ttcgccaaat atcctggata
     3901 tcttccatca gatagccacg atagagcaga aaacgctgga tcttaacttt ttctgaaaag
     3961 acagttggca aaggttcgcc atattttcgc gtcgcctgat cgcgcgccag tgcgcaccag
     4021 tcgatgtcac attcacgcat cgctttttct gtcgcttcgc gggaaatacc tttctgattc
     4081 agttcctgac gaatacgcgc aggtccataa cctttgcggc tacggctggc gataaagcgc
     4141 gcaacaaatc ggctgtcatc gagatagcca tgttcatggc accaggcgat aacgcgctcg
     4201 taatcttctg ccgtggcatc aatctcttcc gggccatttt tgcccatgat cggtgccgcg
     4261 agtttacgtc gcaattcttg ctcactgtga tcgcgcaccg ccagaatgcg taccgcacga
     4321 tccaacaggc gagcatatgc cgggcgacgg gacgttgatt ctgtcatggc atatccttac
     4381 aacttaaaaa agcaaaaggg ccgcagatgc gacccttgtg tatcaaacaa gacgattaaa
     4441 aatcttcgtt agtttctgct acgccttcgc tgtcatctac agagaaatcc ggcgttgagt
     4501 tcgggttgct cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt
     4561 tatctttcag ccaggcagtt gcattcgctt taccttgacc gatcttctca cctttgtagc
     4621 tgtaccacgc gcctgctttc tcgatcagct tctctttcac gcccaggtca accagttcgc
     4681 cgtagaagtt gataccttcg ccgtagagga tctggaattc agcctgttta aacggcgcag
     4741 cgattttgtt cttcaccact ttcacacggg tttcgctccc caccacgttt tcgccctctt
     4801 tcaccgcgcc gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac
     4861 caccggtagt ggtttccggg ttaccgaaca ttacaccaat tttcatacgg atctggttga
     4921 tgaagatcag cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc
     4981 tcatcatacg tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt
     5041 tcggcgtcag tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca
     5101 gggcgtcaca gatttccagt gcctgctcgc cggtatccgg ctgggagcac agcaggttgt
     5161 cgatatcaac gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa
     5221 acgcacaggt tttaccttcg cgctgcgctg cggcgatcac ctgcaacgtc agcgtggttt
     5281 taccggaaga ttctggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc
     5341 caagcgcgat atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt
     5401 cttcacccag gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg
     5461 ccgccaacgc tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta
     5521 ataccggata gtcaatatgt tctgttgaag caattatact gtatgctcat acagtatcaa
     5581 gtgttttgta gaaattgttg ccacaaggtc tgcaatgcat acgcagtagc ctgacgacgc
     5641 accgcatcac ggtcaccgct gaaacattcc cgccgggtaa tgccttcacc acgggcagtg
     5701 gcaaaagcaa accagacggt gccgacaggt ttctcttcac tgccgccatc cggcccggcg
     5761 ataccactaa tagacacggc gtaatcagca cgagccgctt tcagtgcgcc tatcaccatt
     5821 tccaccacga caggttcgct caccgcgcca tgctgtgcca gcgtctcttc gcgtacaccg
     5881 atcatttgcg cttttgcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggaa
     5941 ctgccggcaa tatcggtaat cactttcgct acccaaccgc cggtacaaga ctcagcagtt
     6001 gttacggttg cgccacgggc tttcagcgac tgcccaacct gttcgcttaa ctgcatcagt
     6061 tcactgtcag tcatcacaac ctctgtcagt caaaaattta tgccggacaa gataacactt
     6121 tcgtcaggaa gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat
     6181 tagccagagg gaagctcacg cccccctctt acaaatagtt actgtacgcg cgccagcgcc
     6241 acggcttgtc ctaactgcca gaccgccatt gcgtaatggg tgctgtggtt ataacgggta
     6301 atggtgtaga agttcggcag accgtaccag tactggtagc cggtgccaac atccagacgc
     6361 agcaggctgg cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgccgcggca
     6421 agctgcgaaa tgctgtactt ggttttgaag ccatttggca agcctggagc ctgaccgttt
     6481 gccatcaccg caacctgatc acctttcacc cagccgtgcg ctttgaaata gttcgccaca
     6541 ctaccaatgg catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc
     6601 gcatattgtt tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa
     6661 cctttcagat tgagcggatc gtcctgctcg tcgcgcgcca tcagcaggaa ggtttccagc
     6721 tcgccagaaa aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg
     6781 aggatgcgag ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg
     6841 ataatttccg gcggtacacc atacacctgc cacgcgcgat tcaacgcatc ttcatactga
     6901 ttccagaaaa ccacaccgtt ctgcacgttg tccggcgtaa taaatttttt gcgataacgc
     6961 agccatgcgc cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc
     7021 atcagccgca gtaccgaatc cagacgtttc gcctgggaga gaatttcctg caactgctga
     7081 cgatcgaaac cgtgtttgtt caccattttg tcgatgaact gctgggcatt cggattattg
     7141 gcgaagtcac cgcccatctg catcacattg tgctgcggct caagcaggaa gccgccagac
     7201 ggcgttccgg tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc
     7261 aacacaaaaa ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac
     7321 agattcaacc aggggcaagt atggtaaagc atcacgcccc gcacaaggaa gcggtagtca
     7381 ctgcccgata cggactttac ataactcaac tcattctcct cgctatcctt ttattcaaac
     7441 tttcaaatta aaatatttat ctttcatttt gcgatcaaaa taacactttt aaatctttca
     7501 atctgattag attaggttgc cgtttggtaa taaaacaata aatcctgaag gagagaacaa
     7561 tgatagaaac cattactcat ggtgcagagt ggtttatcgg gctgttccaa aagggcggag
     7621 aggtgtttac cgggatggtg accggcattc ttccgctgtt gattagtctg ctggttatca
     7681 tgaacgcact gattaatttt atcggtcagc atcgtattga acgttttgct caacgttgcg
     7741 ccggtaaccc tgtttcccgt tacctgctgt taccgtgcat tggcacgttt gtcttttgca
     7801 atccgatgac cttaagcctg ggtcgcttta tgccggaaaa gtacaaaccc agctactacg
     7861 cggccgcctc ttatagctgc cactcaatga atggcctctt cccccatatc aacccaggcg
     7921 aactgtttgt ttatcttggc attgccagcg gtctgacaac actgaacctg ccacttggcc
     7981 cactggcggt gagttatctg ctggttggtc tggtcaccaa cttcttccgc ggctgggtga
     8041 ccgatctgac caccgccatt tttgagaaaa agatgggcat tcaacttgaa caaaaagttc
     8101 atctggcagg agcaacatca tgacgcgtat tcggatcgaa aaaggaacgg gtggctgggg
     8161 cggcccgctt gagctggaag ccacgccggg caaaaaaatc gtctatatca ccgccggtac
     8221 ccggcctgcg attgttgaca aactggcaca gcttactggc tggcaggcta ttgacggatt
     8281 taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac
     8341 attacgctgc ggcatttatc cgaaacggcg tattcccacc attaatatcc actcgacggg
     8401 caagtccggt ccactagcgc aatacattgt agaagatatt tatgtctctg gcgtaaaaga
     8461 agaaaacatc actgtagtgg gtgatgcgac accacaaccc tcttccgtgg gccgtgacta
     8521 tgacaccagc aagaaaatca ccgaacaaag cgatggttta ctggcgaagg tgggaatggg
     8581 tatgggttct gccgttgccg tgttgtttca atctggtcgt gacaccatcg acactgtatt
     8641 aaaaaccatt ctgccgttta tggcattcgt ttcggcgctt atcggcatca ttatggcttc
     8701 cggtcttggc gactggattg cccacggtct tgctccgctg gcgagccatc cactgggtct
     8761 ggtcatgctg gcgctcatct gctccttccc gctgctttca cctttcctcg gcccaggcgc
     8821 agttatcgca caggttatcg gcgtattgat tggcgtgcag attggtctcg gcaatattcc
     8881 gccgcatctg gctttacctg cactgtttgc catcaacgcg caggcggcct gcgacttcat
     8941 cccggtcggt ttgtcgctgg cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc
     9001 tgttctggtg agccgctttt taaccggtgc gccaactgta ctgatcgcct ggtttgtctc
     9061 cggttttatc tatcaataga ggctgaaaca tgaccgttat ttatcagacc accatcaccc
     9121 gtattggcgc gagtgccact gacgccctca gcgaccagat gctcatcacc tttcgtgaag
     9181 gcgcgccagc ggatctcgaa gagtattgct tcattcattg ccacggcgag ttgaaaggtg
     9241 cactccatcc cggtttgcaa ttttcactcg ggcagcatcg ctatccggtg accgccgttg
     9301 gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt
     9361 taaacgaagc ggaatttccg ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg
     9421 cgccgggatc ggttttgaag tttgaatctg ttaaggagta aaaaatgaat caggttgccg
     9481 ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg ccacggtctg gctgccgagg
     9541 ggtatcgcgt cgcggttgtc gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa
     9601 ttaacgccga atatggtgaa ggtatggcgt acggttttgg tgctgacgcc actagcgagc
     9661 aaagcgttct ggcgctctct cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg
     9721 tctacagcgc cggaatagca aaagcagcct ttatcagcga cttccagctc ggcgattttg
     9781 accgttcgct acaggtgaat ctggtgggtt atttcctgtg tgcccgtgaa ttttcgcgtt
     9841 tgatgatccg cgacgggatt caggggcgca ttattcagat caactcgaaa tccggcaaag
     9901 tgggcagcaa acacaactct ggctacagcg cagcgaaatt tggtggcgtc gggctgactc
     9961 aatcactggc gctggatctg gcggagtacg gcattacggt gcattcactg atgctcggta
    10021 atctgctgaa atcgccgatg tttcagtcgc tattgccaca atatgcgacc aagctgggta
    10081 tcaaaccgga tcaagtcgag cagtattaca tcgacaaagt accgctcaaa cgcggctgcg
    10141 attatcagga tgtgctgaat atgctgctgt tctacgccag tcctaaggcg tcgtactgca
    10201 ctggtcagtc gatcaatgtc accggcggtc aggtgatgtt ctgattaaca gcggagatcc
    10261 gttaaggatc tccgcgagac taataaaatg cctgatgcgc tacgctcatc aggcatacag
    10321 gacttccgcc actacattaa ggaaaagtta tggtatccgc actcatcacc gtcgccgtta
    10381 tcgcctggtg tgcgcaactg gccttaggcg gttggcaaat ttctcgtttt aaccgtgcct
    10441 tcgacacact atgccagcaa gggcgggttg gcgtgggccg ttccagcggg cgctttaaac
    10501 cgcgggtcgt ggtcgccatc gccctggacg atcagcaacg catcgtcgac accttgttta
    10561 tgaaaggact gaccgtcttc gcccgaccac aaaaaattcc cgcaattacc ggtatgtatg
    10621 cgggtgattt acagcccgat gtgatctttc cccatgatcc actatcacag aatgctctat
    10681 cattggcgct taaactgaaa cgtggataat ttcgttgtga atgttacttg cttgcgaagt
    10741 tatcattttg aaacctaaat caggtaatca cgcccatgaa acctcgtcag cgtcaggccg
    10801 ccattctgga gtatctgcaa aagcagggta aatgctcggt tgaagaattg gcgcaatact
    10861 ttgacaccac aggcacaacc attcgcaaag atctggtcat tctggaacat gccggaaccg
    10921 tcattcgtac ttatggcgga gtggtgttga ataaagagga atccgatccg ccaatcgatc
    10981 ataaaacact catcaacacc cacaagaaag agctgattgc agaagctgcc gttagtttta
    11041 tccatgatgg cgattcgatc attcttgatg ctggcagtac cgttttgcag atggtccccc
    11101 tgctctcgcg ctttaataac atcacagtga tgaccaacag cctgcatatc gtcaatgcgc
    11161 tatccgaact ggataacgaa caaactatcc tgatgccagg cggaacattt cgtaaaaaat
    11221 cggcctcatt tcacgggcaa ctggcagaga atgccttcga gcatttcacc ttcgataaat
    11281 tgtttatggg caccgacggc atcgatctca atgcgggcgt aaccacgttc aacgaggttt
    11341 ataccgtcag taaggcaatg tgcaatgccg cgcgcgaagt gattttgatg gcggactcat
    11401 caaagtttgg ccgtaaaagc cccaacgtag tttgcagtct tgaaagcgtc gataagctaa
    11461 ttaccgacgc aggtatcgat ccagcgtttc gtcaggcgct ggaaaagaaa gggatcgacg
    11521 tgatcataac cggagagagc aatgagtgaa gcactactga acgcgggacg tcagacgtta
    11581 atgctggagt tgcaggaagc aagccgttta ccggaacgtc tgggcgatga ttttgttcgc
    11641 gccgccaata tcatcctgca ctgcgaaggc aaagtggtgg tttcgggaat tggcaaatcg
    11701 ggccacattg gtaagaaaat cgccgcaacg cttgccagca ccggcactcc tgcctttttt
    11761 gtccatccgg cagaggcatt gcacggcgac ctggggatga tcgaaagccg cgatgtgatg
    11821 ctgtttatct cttactccgg tggcgcgaag gaactggatc tgattattcc gcgtctggaa
    11881 gataaatcta tcgcgctgct ggcgatgacc ggcaaaccga cgtcaccgct gggcctggcg
    11941 gcgaaagcgg tgctggatat ctccgtagaa cgcgaagcct gcccgatgca ccttgcgccg
    12001 acctccagca ccgtcaatac cctgatgatg ggtgacgcgc tggcgatggc ggtcatgcag
    12061 gcgcgcggat ttaatgaaga agattttgcc cgctcccacc ctgctggagc actgggcgct
    12121 cgcttgctga ataaagtgca tcatctgatg cgccgtgacg atgccatccc gcaggtggcg
    12181 ttaaccgcca gcgtgatgga cgcgatgctg gaacttagcc gcaccggttt aggtctggtg
    12241 gcagtgtgcg atgatcaacg actggtgaaa ggcgtcttta ccgacggtga tttacgtcgc
    12301 tggctggttg gcggcggcgc actcaccacg ccagtcaatg aagcgatgac ggtcggcggc
    12361 accacgttgc aatcgcaaag ccgtgccatc gacgccaaag aaatcctgat gaaacgcaaa
    12421 atcaccgccg caccggtagt ggatgaaaac ggcaaactca ccggcgcaat taacctgcag
    12481 gatttctatc aggccgggat tatttaatcc ttcagcccca gacgtttcgc cagccgatgc
    12541 aggttggcga cgtcggtttc cagcatccgc gcacaggcgg cccagttgtg atgattttgc
    12601 gccagtgcct gacgaatggt ttcacgctgg aacgcttctg tcgcttcacg caggttttgc
    12661 ttaacgatag gcaccgccgc cgcttctggc ggcggcaacg tcacctcagg aaaagcaaaa
    12721 tgttgcgcct caagaatcac ttcatcgccg ctgcgagtgg ctctcgacag tactaccgcc
    12781 cgatgaatag catgttccag ttcgcgcacg tttcccggaa aactgtagtg ttgcagtaaa
    12841 tttcgcgccc cggcacttaa taccacgcgg gagagcccca gccgcaaacg acactgctcg
    12901 cagaaatacc ccgccagcag aatgacatca tcgccccgct cacgcagcgg cggcaccgaa
    12961 agtggaaaca cgctcaggcg atgaaacaaa tcggcgcgga atcgccctgc cagcacctct
    13021 tcgcgtaaat cgcggttagt cgccgccagc acgcgcacat cgacccgcaa actgcggtca
    13081 tcgccaacgc gctgaatatc gccatactgc aacaccctca gcagcttggc ctgcaatgcc
    13141 aacgacaact cgccgatctc atcgagaaac agcgtgccgt tatccgccat ttcaaacttc
    13201 ccgctgcggt tactgatagc accagtaaac gcccctttca catgcccgaa caactcactt
    13261 tccgccacac tttccggcag tgcggcacag ttgagataga ccagcggatt caccgcccgt
    13321 ggcgaggctt catgaatcgc tttcgccacc agctccttac cggttcccgt ctcaccgcta
    13381 attaaaacgt tgagatcgga tgccgccaca atctcaatct cttttttcaa ttgcgtcatg
    13441 cctggggaca aaccaatcat ctgcgtctgt ttcaccgctt caaacggcgc ggcatcgcct
    13501 ggcagcatat tctggctttc cagttgttca atcagtaacg cattacttaa cgctcccgcc
    13561 gccagtgcgg caatcagccg tagctcttcg tcgctgaaaa catcgaactg atcgggctgc
    13621 atcccgtcga gcgtcaaggc accgatcagg ttttgcccgg caaacaacgg caaaccaacg
    13681 caggcgtgaa ccttcagact ctcctgccct ggaatcaaac cgtcataagg atcgggcaat
    13741 tcgctgtctg cgggaaagcg caccacatca cccgcgcggg caatcgcttc cagccgtgga
    13801 tgaccttcca gcgcaaagcg tctaccgagt acatccttcg ccagcccgtc gatggcaagc
    13861 ggaataaact gccgcgagtc gtaacgtagc aacgcagacg catcgcactc cagcacctga
    13921 cgcagcgtgg tgatcaggcg ctgaaaacga tcctgatgac caatcccacg ctgcaattcg
    13981 atggcgatat tcgccagcac atcaacggaa aaactcatct ttgcctcact gtcaatttga
    14041 ctatagatat tgtcatatcg accattttat tgatagtcat tttgactact cattaatgag
    14101 cacaatttta tttatagagt aaaaacaatt agataaaaaa ctggcacgca atctgcaatc
    14161 agcaagacat ctttttagaa cacgctgaat aaattgaggt tgctatgtct attgtggtga
    14221 aaaataacat tcattgggtt ggtcaacgtg actgggaagt gcgtgatttt cacggtacag
    14281 aatataaaac gctgcgcggc agcagctaca acagctacct catccgcgaa gaaaaaaacg
    14341 tgctgatcga caccgtcgac cataaattca gccgcgaatt tgtgcagaac ctgcgtaatg
    14401 aaatcgatct gacggatatc gattacatcg taattaacca tgctgaagag gaccacgccg
    14461 gggcgctgac cgaactgatg gcacaaattc ccgatacgcc gatctactgt actgccaacg
    14521 ctatcgactc gataaatggt catcaccatc atccggagtg gaattttaat gtggtgaaaa
    14581 caggcgacac gctggatatc ggcaacggca aacagctcat ttttgtcgaa acgccaatgt
    14641 tgcactggcc ggacagcatg atgacttacc tgacaggcga cgcggtactg ttcagtaacg
    14701 atgctttcgg tcagcactac tgcgacgagc atctgttcaa cgatgaagtg gatcagacgg
    14761 agcttttcga gcagtgccag cgttattacg ccaatatcct gacgccgttc agccgcctgg
    14821 taacgccgaa aattaccgag atcctgggct ttaacttacc agtcgatatg atagcgacct
    14881 ctcacggcgt ggtatggcgc gataacccga cgcaaattgt cgagctgtac ctgaaatggg
    14941 cggcggatta tcaggaagac agaatcacca ttttctacga caccatgtcg aataataccc
    15001 gcatgatggc cgatgctata gcccagggta ttgcggaaac cgacccacgc gtagcggtga
    15061 aaattttcaa cgtcgcccga agcgataaaa acgaaatcct gaccaatgtc ttccgctcaa
    15121 aaggcgtgct ggtcggcact tcgacgatga ataacgtgat gatgccaaaa atcgccgggc
    15181 tggtggagga gatgactggt ttacgctttc gtaacaaacg cgccagtgct ttcggctctc
    15241 acggctggag cggcggtgcg gtggatcgtc tttccacacg cctgcaggat gcgggtttcg
    15301 aaatgtcgct tagcctaaaa gccaaatggc gaccggacca ggacgctctg gagctatgtc
    15361 gtgaacacgg tcgcgaaatc gcccgtcagt gggcgctcgc gccgctgccg cagagcacgg
    15421 tgaatacggt ggttaaagaa gaaacctctg ccaccacgac ggctgacctc ggcccacgga
    15481 tgcagtgcag cgtctgccag tggatttacg atccggcaaa aggcgagcca atgcaggacg
    15541 ttgcgccagg aacgccgtgg agtgaagttc cggataactt cctgtgcccg gaatgctccc
    15601 ttggcaaaga cgtctttgac gaactggcat cggaggcaaa atgagcaacg gcattgtgat
    15661 cattggttcg ggcttcgccg cccgccaact ggtgaaaaat attcgcaaac aggacgcctg
    15721 tattccatta accctgattg ccgccgacag catggatgag tacaacaaac cggacctcag
    15781 ccatgttatc agccagaggc aacgtgccga tgaccttacc cgccagacgg cgggtgaatt
    15841 tgccgagcag tttaatctgc acctgtttcc gcacacctgg gtaacggata tcgatgccga
    15901 agcccatgtg gtgaaaagtc agaataatca gtggcaatac gacaagttag tgctggcaac
    15961 cggtgccagc gcctttgtcc cgccagtgcc cgggcgtgag ttaatgctga cgttaaatag
    16021 tcagcaagag tatcgcgcct gtgaaacgca actgcggaat gcccgacgcg tgttgattgt
    16081 tggcggtggc ttgattggta gcgaactggc gatggatttt tgtcgtgcag gcaaagcggt
    16141 cacgctaatc gacaacgctg ccagtattct ggcagcgtta atgccaccgg aagtaagcag
    16201 ccgcttgcag catcggttga cggagatggg cgttcatctg ctgttaaaat ctcagttgca
    16261 ggggctggaa aaaacggatt ctggcattct ggcaacgctg gaccgccagc gcagcatcga
    16321 agtggatgcg gtaattgccg ccaccggact gcgcccggaa accgccctgg cacgacgcgc
    16381 cgggctgacg attaatcgcg gcgtttgcgt cgatagttat ctgcaaacca gtaatgccga
    16441 tatttatgcg ctgggcgatt gcgcggaaat taacggtcag gtattgccgt tcctccagcc
    16501 gattcaactt agcgcgatgg tgctggcaaa aaatcttctc ggcaataaca cgccgctgaa
    16561 actcccggcg atgctggtga aaatcaaaac gccggaatta ccgctgcatc tggcaggcga
    16621 aacccagcgt caggatttac gctggcaaat taataccgaa cgccagggaa tggtggcgcg
    16681 cggtgttgat gatgccgacc agcttcgcgc ctttgtggtc agtgaggatc ggatgaaaga
    16741 ggcgtttgga ttgttgaaaa cgttgccggt gtaggcgagc tacagtgccc caaatgtcgg
    16801 atgcggcgct gacgcgtctt atccgaccta cagggaacgc atgtgttgat cacataatac
    16861 gtttatgtcg catcctgcac tgtcccaaaa tgtcggatgc gacgctggcg cgtcttatcc
    16921 gacctacggg gacgcacgtg taggccggat aaggcgttta cgccgcatcc ggcaatggtg
    16981 tccaaatgca acacgcttta tccgttctgg acttcacccg ctaaccagcg cgccgcagca
    17041 ataaccccct gccccagaga caatccacca tcgcccgccg gtaaactctg cgggaagagc
    17101 aacgtaaaat cagcaagata atgcgccaga cgtgcacgca gcaaacggtt atgaataact
    17161 ccgccgctaa ataccagcgt agtgataccc cgcatcgtgg cctgctcacg catcaacgcg
    17221 gcaaaaccct gcgccagcgc atcatgaaac gcccacgcgc gttgattaac cggagcctgc
    17281 cagctcagcc actgctgcca gaaagtggcg agatccagtt gattgtccac cagcggcatc
    17341 gtcaccggat gcgtcactcc gtcgcacgaa gctgcgagcg cctccagagc acaagccgct
    17401 tcaccttcat aacttaacgt ggctggcgca cagcccagtc ccgccgccac agcatcgaac
    17461 aaacgcccac acgatgacgc cagcggcgcg ttaattccac gctcaatggc ccgcgccagc
    17521 acgctccagt tttgctgttg cacacttgcc gtttcagggt aattctgcca ctccggcaca
    17581 aagcgcaggc actgcgccag caggttacgc cacggctgct tcgccgccaa atcgccccca
    17641 gcaagcgcca ctgcaggcaa gccgcccagg tgctggcatt cgcgatagtt cacccgcagg
    17701 cactcgccgc cccacaaagc gccgttctcc cccataccga taccgtcgag cgtcaacgca
    17761 atgacatcac cgccatccag tggccagtga tgttctgcca gacacgccgc cgcatgagca
    17821 tgatgatgca gcaccgtttg cgtcggcaga ttcatctcac gcgcccactg gctggagaca
    17881 tagcccggat gcgcgtcatg cacaacgtat tgcggggtga aatcgtagat gttttgcatc
    17941 aggcgtaacg cttcgcgcca ctgcatctgg atgccatcgt cacttaaatc gcccagatgc
    18001 tggctcaaca ccgcttgttc accgcgcacc aggcagaagg tatttttcag atccgcgccg
    18061 agacacagca caggcggaac atttttaaag cccggaggca aagccagcgc atccggcaca
    18121 tacccccgcg aacggcgcag catttcgccg ctttcgcgca ccaccgaatc atccatccgc
    18181 tgcacgatgt cgcggttatg tatcaagaat ccgtcggcaa tgccctgcaa atccgccagc
    18241 gcctgttcgt tgctgatagc cggtggttta ccgctcaggt tgccggaggt catcaccagc
    18301 gggcattgca gttcctgtaa cagcaaatgc tggagcgggt tcgcaggcaa catcacccca
    18361 acttcgttaa ggccaggggc gatatcatca caaagctcag gaacgtattt tttatccacc
    18421 agcacaatcg gcgcggcggg tgtggtaagc aactgacggg cagcgtctgg taaaccttct
    18481 gccactggca acataaccgc cagcggtttc gccgggcgat gtttgcgcgc ccgcagtgtc
    18541 gccaccgcgt tactgttacg cgcatcgcag gcgagatgaa atccgccaat ccctttgatg
    18601 gcgacaatgt tgcccatttt taactgtgcg atagctgcct gtaatgccgc ctcttgttct
    18661 gcatgttcac catgacttac ccattcaaga tgggggccac actccgggca ggccaccggc
    18721 tgggcgtgga agcgacgatc gagcgggtca cggtactctt tgtcacaggc cggacacagc
    18781 ggaaacgccg ccatcacggt aaacgggcgg tcgtaaggca tggcgcgaat aatggtgaaa
    18841 cgcggaccac agtgagtaca gttgataaac ggataacgcc agcgtcgttc gcctggggta
    18901 ttcatttcgg caagacaagc agggcaagtc gcggcatccg ggacaatttg cgtattcatg
    18961 acgccacccg cgctctggcg gatggtgaac tcagtgggca gttgtgacca gataaacggc
    19021 tcacgctcga cgctatcaat acgcgccagt ggcgggcagt gctgatgcaa ctgcacaaga
    19081 aacgtttccg gatcttccag cagccggact tctacgccat cgccgtcatt acagacatcg
    19141 ccgtgaagat ttaattgctg tgccagttgc cagacaaacg gacgaaaacc gacgccctgc
    19201 actttgccac gaatacgcag ttggacaccg caagatgtgt tttttgccat tgagttattc
    19261 ccgccatcat gaattgcgta acccgcccag ccggacacga cagcgtcgca tccggcagtc
    19321 acaggtcggc gatactgccc gcttcgtatt ctacgaatat ttccgggaat tcctttgatg
    19381 ccagaacagt tcagtaagat ttttagaaca tcagcgccgt acggcgacgt ttttctgcgc
    19441 tcagttgttc aagtttatta cgatcgacac aaatcagcgc atgggtcggg caagccgcca
    19501 tacacgccgg gccgtcttca cgatggttgc acaggtcgca tttattggct tcggctttgt
    19561 cagcccgtac attcagcccc gcgccgctgt tgcgaatcac cggacgtacc accacttcca
    19621 tcgcgccata cgggcaagcc acaacgcagg ttttgcaacc aatacaacgt tcctgcatca
    19681 catgaacaaa ccctttatca cgactgatag caccattcgg acagacgtta gcgcacggtg
    19741 catcttcaca ctgacggcaa actgtcgccg tggaaatgtt cacaccttta atgacatgga
    19801 ttcgcggtaa aaaagtttcc ggggtcagcg atgcacagtc ctgattttcc tgatgagaaa
    19861 ccacgcacgc cacttcacag gtacggcaac caatacattt actcgcgtca gcaatgatga
    19921 aacggttcat caaattctcc agcaatgaca gttaatgcgc cgatacattc acaaatcatg
    19981 ccagttttta atttactgtt atttaaagaa attaaattct gtaatgcagg aaaaacgatg
    20041 tcatcgacac tagtgacgat gacatgtgat gacaatgttt atcgcgaaag agcaatgagg
    20101 gagtcgcggc ggatcagttt tccgctgaag gttttcggcg gtgtgtaagc accaccatcg
    20161 agcataaata tcagccgccc aattatttcc tgaatcatct cagtcaccgg aatttttacg
    20221 ctggagagcg ccggaacggt gtagggggca atagcgatat catcgaatcc gataactgac
    20281 acctgctctg gcaccgctac gccgcgctcg tgtaacgctt tcatcgcacc tatcgccata
    20341 tcgtcgttac tggcaactaa cgcgctaaat ttagccccac gttcgagcaa catttctacc
    20401 ccttcggccc cgctggcagg cgtccattta ccgttagcga taagtttttc attgagcgca
    20461 ataccatgct gcgccagcgc gtctttatac ccggcaagac gttcaacgct ggtgggggaa
    20521 tccatcgaac cggtaaggaa agcaatctcc tgatgtccgg cgtttatcaa ctctgccacg
    20581 gcgttaaagc tggtctgttt atgatcgcac cagacgctat ggctgctgtt tttgcgcagg
    20641 cggcgattaa gcaccattat cggctgactg tgtgcgtcaa tgatgtcatc aatctcatcc
    20701 acgcttaaaa aacgcgggta aatcataatg gcgtcgcagc gcaaatccag cagatactga
    20761 atcgcctggc gctcttcttc ggcgctgtgt ttaccatctg ccaatagcaa ctgtcgccct
    20821 ttctcttccg ccattcgcgc ggcatgaaag agtaattcac taaaataaat gccgtggtaa
    20881 agcgtgttgg tcacgaccag ccccagcgtc tgagtactct tcgccgacag attgcgcgcc
    20941 agcaagtttg gacggtaacc gctctcttct accgcctgaa acacgcgatc tttagtctcc
    21001 tggctgacgt agccattacc tgaaagcacg cgggaaacgg tcgcttttga aaccccggcg
    21061 cgcttcgcca cttccagcat cgtcgtcatc attttcatcc ctttacacgc aatcaacgca
    21121 gtgtactgca ccgtttgccg attgtccttg cgcaatcggc gggaaaaata ttcaggtgac
    21181 cggtttcaca aatataaaaa atgaacaatt aactctcttg cttattcagt gacaactatt
    21241 catgattttg tgaaaccggt ttcctaattc cgtttcagca tcggcatttt tccgtcacgt
    21301 cgactgataa caactacatc taccctactg ataacaggat aaaatccgat ggacaaaaat
    21361 tatgcggcgc tggcacgctc ggtgatagcg gcactgggcg gcgttgataa catctcggca
    21421 gtcacgcact gtatgacgcg tttgcgcttt gttatcaaag atgaccagct tatcgacagt
    21481 ccgacgttga aaaccatctc cggcgtgctc ggcgtggtac gtagtgacaa ccagtgtcag
    21541 gtgattatcg gcaataccgt ttcacaagcc tttcaggaag tcgtcagcct gctgccggga
    21601 gatatgcagc ccgcacagcc cgtgggtaaa cccaaactta cgctacgtcg cataggtgcg
    21661 gggatcctcg atgcgctgat cggcaccatg tcaccgctga tcccggcgat tatcggcgga
    21721 tcgatggtca aactgctggc gatgatcctc gagatgagcg gcgtgctgac aaaaggatcg
    21781 ccgaccttaa ccattctgaa tgtgattggt gacggtgctt tcttcttcct gccgctgatg
    21841 gtcgcggctt cggccgccat caaatttaaa accaatatgt cgctggcgat tgccatcgcg
    21901 ggtgttctgg tacatccgag ttttattgaa ctgatggcga aagcggccca gggtgaacat
    21961 gtcgaatttg ccctgatccc ggtcaccgcg gtgaaataca cctacacggt gatcccggcg
    22021 ctggtgatga cctggtgcct gtcatatatc gaacgctggg tggatcgcat tacaccggcg
    22081 gtgactaaaa acttcctcaa gccaatgctg attgtgttga ttgccgcacc gctggcaatc
    22141 ctgctgattg gcccgattgg tatctggatc ggtagcgcca tttcggcgct ggtttacacc
    22201 attcatggtt atctgggctg gctttcagtc gccattatgg gcgcgctgtg gcctctgctg
    22261 gtaatgaccg ggatgcaccg cgtctttacg ccaaccatca ttcagaccat tgccgaaacc
    22321 ggcaaagaag ggatggtcat gccgtcagag atcggcgcta acctgtcgct gggcggttca
    22381 tcactggcag tggcgtggaa aacgaaaaac ccggaactgc gtcagacggc gctggctgcg
    22441 gcggcatcag ccattatggc ggggatttcc gaaccggcgt tatatggcgt ggcgatccgc
    22501 ctgaaacgtc cgcttatcgc cagtcttatc agcggtttta tttgcggcgc ggttgccggt
    22561 atggcggggc ttgccagcca ctcaatggca gcgccggggc tatttaccag cgtgcagttc
    22621 ttcgatccgg cgaatccaat gagcatcgtc tgggtgttcg ccgtcatggc gctggcggtc
    22681 gtgctgtcgt tcatcctcac actgttgctc ggctttgagg atattcctgt tgaggaagcg
    22741 actgccgagg cgcgaaagca tcagagcgca caaccgaccg tcgccaaaga agtaagtctt
    22801 aattgaggat gaaaatgtca gtatttccag aaggtttttt atggggcggc gcgcttgccg
    22861 ccaaccagtc tgaaggtgcg taccgggaag gtggcaaagg gctgaccacc gtcgatatga
    22921 tcccacacgg cgagcatcga atggcggtga aactggggct ggaaaaacgt tttcagttgc
    22981 gcgatgacga gttttatccc agccatgagg cgacggattt ttatcatcgt tataaagaag
    23041 atatcgccct gatggcagag atgggattca aagtgttccg tacctcaatt gcctggagcc
    23101 gcctcttccc gcagggcgat gaactgacgc ccaaccagca gggcattgct ttttaccgtt
    23161 ctgtctttga agagtgtaaa aagtacggta tcgaaccgct ggtcacgttg tgccacttcg
    23221 atgtgccgat gcatctggtc accgaatatg gctcctggcg taaccgcaag ctggtggagt
    23281 ttttcagccg ctacgcccgg acctgctttg aagcatttga tggtctggtg aaatactggc
    23341 tcaccttcaa tgaaatcaac attatgttgc atagcccgtt ctccggcgcg ggtctggtgt
    23401 ttgaagaagg cgaaaatcag gatcaggtga aatatcaggc tgcgcatcac caactgattg
    23461 ccagtgcatt agccaccaaa atcgcccatg aggttaaccc gcaaaatcag gtgggctgta
    23521 tgctggcggg cggtaacttc tacccttaca gttgcaagcc ggaagatgtc tgggcggcgc
    23581 tggagaaaga tcgggaaaac ctgtttttta tcgatgtgca ggcgcggggc gcgtatccgg
    23641 cttactctgc ccgcgtattc cgcgaaaaag gggtaaccat cgacaaagca ccgggcgatg
    23701 atgagatcct gaaaaacacc gtcgattttg tctctttcag ctattacgcc tcacgctgcg
    23761 cctcggcgga gatgaacgcc aacaacagca gtgcggcgaa cgtggtgaaa tcgctgcgta
    23821 acccatatct acaggtaagc gactggggct gggggattga tccactcggt ctgcgtatca
    23881 ccatgaacat gatgtatgac cgctatcaga agccgctgtt tctggtggaa aacggcctcg
    23941 gcgcaaaaga tgaacttgct gccaatggcg agattaacga tgactatcgc atcagctatt
    24001 tacgcgaaca tatccgcgca atgggcgaag cgattgcaga tggcattccg ctgatgggct
    24061 acaccacttg gggctgtatt gatttggttt ccgcctcaac gggtgaaatg agcaaacgct
    24121 acggttttgt ctacgtagac cgtgacgacg caggcaacgg tacgctggcg cgcacgcgta
    24181 agaaatcgtt ctggtggtat aaaaaagtga ttgccagtaa tggtgaggat ttagagtaag
    24241 taacagtgcc ggatgcggcg tgaacgcctt atccggccta catgtcgcac gaaccaggta
    24301 ggcccgataa gcgcagcgca tcgggcaatc tggcgtttgc tatcagttct gattccctca
    24361 tcaagaacat ccctgtcctg attccttact gaaaaactac tcttcttcca ccgctaactg
    24421 cgcgaatccg ccattccctt cccagccttc cagtcgttga taaacggttt ctaccgcatc
    24481 tttaatcggc tgggtcatcg ggtagtaaaa gccgacgata tccggctgga tgccgaggaa
    24541 aatcacttcg ccaatgtctt cttttaactg gtcgataagg tagttcaacg gcatgttatg
    24601 ggtagtcatc ataaacatct cggcgatatc atccggatcg atgatgcgga tctcgccggg
    24661 gtttaacccc atatccgtgg cgtcgacaat cagcagccgt gtcgggcgca gttcgcggat
    24721 ggcgacgatg tcgttttccg gagcgctacc gccgtcaatc accacccagt tacctttcgg
    24781 cgcggcggcg cacttttccg ccagcagcgg gcctgcgcca tcatcgccca tcatgctatt
    24841 gccaacacaa agtaaaacgt cagtcacgca gtctcctcac catcaaatag atggcgtttt
    24901 cctgatgaat atcatgcagc atacttaaca gcattgtact ccattctttc tgctgcgaag
    24961 tttgcgctgc ccgggcttta tcgaatgcgt tagccagcat cggcacatgg ttaatgtcga
    25021 tgacgatctc accatatttc ggcacgcctt ccattttgcg gcgggcttcg ctgcccgcct
    25081 ccagagtggc aatccatgcc agatagtcat cccacgggca ggtaagcgcc gcttccaggc
    25141 aatcgataac cccaaggtgg tgaccaatcg ccaggctgta atagaccacc tgctgcgcct
    25201 cggcgggcgt agcatcgttc tcatcaataa atttacggct cagttgactg aacaccacct
    25261 tttcactcat cggatacgcg cctcttcaac aacatgattc agatggctga caatctcgtt
    25321 cagacgcgga tcgttttccg cttccagcca gcgtgcaacc tgttcttcgc cctgccctaa
    25381 ctgtgtaagg taatcatcgg caatctgacg accgtaacga taacccgcca ggcggcgtgc
    25441 ttcgcgatcc actttcacac gcagcggctg caccatatcg ccatgcagga tctccgccgg
    25501 ttgttcatcc tgctcacccg gcccacgggc gtgaattttc tgctccagca ggccgagcgc
    25561 cattgcaaag ccgtatagcg tagcggcagg cgttggcggg cagccaggga tgtagacatc
    25621 taccgggacg attttatccg taccgcccca cacgcagtag agatcgtgga agatcccgcc
    25681 actgttaccg caggcaccgt aggagataca gattttcggg tccggcgcgg actgccacgc
    25741 acgcagcgca ggggatcgca ttgcacgggt gaccgcgccg gtaaacagta aaatatccgc
    25801 atgacgcggt gaaggaacga ctttaatgcc gaagcgttct gcatcaaaca gcggcgaaag
    25861 cgtggcgaaa atttcgattt cgcagccgtt gcagccaccg cagtccacgc ggtaaacgta
    25921 ggcagaacgt ttgatttttt tcagtaacga cgccttcatg ctggcgatgg attcatccac
    25981 cgtcatcggg accggaatgc cgttggcgtc acgggggcct aataaattgc tcatcagatg
    26041 gcctctttca tatggcgagt cagttcaata cggtcggacg gcaccaggca tttctggcgc
    26101 ttacattccg ggcaagtctc aaagctttcg cggtggtttt ccgcgcggct gtcaccattg
    26161 tgcttaagca gcgcaatggc gtagtcgatc tctttctgga cggcgaaagg acgattgcag
    26221 acgcggcagt tgcacagcgc gaagcgggac tgttggagga agtcttcttt cttccacacc
    26281 gccagttcgt actcttgcga cagtttgatc gccgccgtcg ggcaaacttc ttcgcagcgt
    26341 ccacagaaga tgcagcgccc gagattaaac tcccaggcca gctctcctgt ggcgaggtcg
    26401 gtttcaaccg ttaaggcgtt tgacgggcag gcattgacgc aggccgcgca gccgatgcac
    26461 tgctgcgggt tctgctctgg cttaccacgg aagtttttat caaccgcaat cggctccagc
    26521 ggataagacg aggtcgccgt gccggttttg atgacttttt tgataaaggt aaacatggcg
    26581 attccttatt tcagcggcga gtttttacgc tcaatgctgt aacgctcgag ttctttgtac
    26641 ggcaccactt tgctcttctt cttacgcaca tcgaccacgg tcatgcggtc ggtacaggag
    26701 tagcaagggt ccaggctacc gataatcagc ggcgcatcgg aaacggtgtt gccgcgcagc
    26761 atgtagcgca gggtcggcca gttcgcgtag gtcgcggcac ggcagcgcca gcggtacagc
    26821 ttctggttgt cgccggtcat gctccagtgg atatcgtcgc cgcgcggcgc ttcggcaaag
    26881 cccagcgcaa aacggtgcgg aatgtaggta aaaccttcta ccatcaacgg accgcccggc
    26941 aggttatcca gaccgtagtc gatcatgttc agcgcggtat agacctcgtt gattcgcact
    27001 ttcagacggg aaataacgtc gcagccctgc tcgctgtgga cttccattgg cagcaggcca
    27061 taaccgacaa acgggtgatc ggcgcgagta tcgcgggcgt gaccgctggc gcgaaccatc
    27121 gggccaacgt tactgaaatc gcgggcgatt tccgggtcca gacgaccaat gccgacggtg
    27181 cgctgttcca tgttcggtgt gctcagcagc acatccacca gctcctgcac ttcacgacgc
    27241 atctgttgtg ccagctggcg ggtctggatc atgtcgtctt tcagcagatc gcgacgaatc
    27301 ccgccgatca ggttcaggcc gtaggtttta cgtgcatcgg taaggatctc tgccattttc
    27361 atggaggttt cacgcacgcg gaagaactgc ataaagccgg agtcgaagcc ggtaaagtga
    27421 caggcgaggc cgagattgag cagatgcgag tgcaggcgtt ccacctccaa cagaatggcg
    27481 cggatcatct gcgcacgttc tggcacctga atacccatcg cgttttccac cgacgtggtg
    27541 taggcggtgc tgtgagcaaa gccgcagatc ccgcacacac ggtcagagag gaaggtcact
    27601 tcgttgtaac ccatacgggt ttccgccagt ttttccatgc cgcgatggac gtagaacagg
    27661 cggtagtcgg cgtcgataat gttttcacca tcgacaaaca gacggaagtg acccggttcg
    27721 tcagaagtga cgtgcagggg accaatcggc acgacgttgt ttttcttgtc gcccagttcg
    27781 ttgatgaact cgtaggtttc agcatcggta gtcggtgccg gacgctgacg ataatccatg
    27841 ctgtctttac gcagaggata aagttcatcc ggccagtcat ccggcagcac cagacggcgt
    27901 tcatccggca gaccaaccgg aatcaaaccg tacatatcgc gcacttcacg ctcgccccac
    27961 actgccgccg gaacgcgcgg cgtcacggaa ggatactccg gtttgttggc gtcaacttcg
    28021 acgcgcaccg ttacccaaca cttagtcccc ttctccatcg acagcacgta ataaacggcg
    28081 taatgaccat tcagtttgcg ttcgtcgtta ccaaacagca ccgacagcca gcccccctgc
    28141 ttgtagtaga gaaactccac cacttccggc aggtagttca ccttcacggt cacggtcagc
    28201 tgatctttgg tctgccaggc gtggtccagc acgacgcccg gaaatgcctc attcagcgcg
    28261 gcgagataat gttgacctaa tttttcttca gacatgctca aactctcttt aatcacgccg
    28321 ccagcaagga gacgaacgct aaaaatgcaa agccaaaacc agcccaggta atgcgtggag
    28381 taatatcaag acgcaaacgg gccatgctgt tttcgaacag cgcgataacc agtacgccga
    28441 ccaccagttt gacgatggca atcaccagcg ccagcagcag cccaccgacg gtgaaggttt
    28501 ccatttgtcc ccacggaata aacaccccga cgaacatctg caacaccacc agctgtttca
    28561 ggctgatgcc ccatttcatg acgccaaagc cgctgccgct atactcagag agcggacctt
    28621 cctgcaactc ctgctcggct tccgccaggt cgaacggcag tttgcccatt tcgataaaag
    28681 tggcgaacgc acaggcacaa agcgccagca ccagcgggat gctctggctc agtggccagt
    28741 gataaacggt gtcggtgatg ttgctgatgt tggtggaacc ggcaacctgt gcggcgaccc
    28801 acagaccaag cagcagcatc ggttcaacca acacgccaag catcgcttca cggctcgcgc
    28861 cgatagcggt aaacgggcta ccagtatcca gaccagaaat ggcaaagaag aaacgcgcga
    28921 tggcgaagag atacagtaag gtgatcaaat cgcccagttg cggcagcgga gagccgacgg
    28981 tcaccaccgg cagcgcagtg gcgatagtca gcatgacgcc caccatcaca tacggcgtca
    29041 ggcggaacac ccagccggag gcatccgggc caacgctctg acgccccagc agtttgataa
    29101 tgtcgcgata ctcctgcaac acgcccggcc cacgacggtt atgcaagcgg gcgcgcgcta
    29161 cgcgggtaat accggagagc agcggcgcaa cggcaaataa caccagcgcc tgaattaacg
    29221 gatataaaac actcattctc aggctcctcg tgaaacaata atcaccacca gtaccgccag
    29281 ttcaaccagc gccatccggc ggaacagcaa cgcactcccc tcgcactgcc agcccggcac
    29341 cagcgacacc ggattcagcc atttgcgtag tttcagcacc ggcgcaaacg cctgtttcac
    29401 cggcatggca aaaccgtgag cggtaatcac cattgatttt tcatgatcgt agccgcacac
    29461 ccaggccgcg ccgcgggaac gcgacggcaa acgatcgcct ttgcaaatcg ccataatgat
    29521 gaatggcagc agcgggcagg caatcagcag caacgtgatc atgggttgag aaacggtggt
    29581 gttagcaggc tccagcggca gaggtacagc agcagagagc atcggcagta gccacggcgc
    29641 agcaacaccg ccaattacgc agcaaatcgc cagtgccact acgcttacgc tcatcaggag
    29701 cggcgcacag gtggcgtttt cggcctcttt ggtacgcggc gcgccgagga aagtaacgcc
    29761 ataaactttc gccatacaca tcaccgccag cgcaccggta attgccagcc ccacggcgag
    29821 cagcggcccg agaagacggg caacaaacgc gccactattg ctcagtttga agaaggattg
    29881 atagataacc cattccccgg caaacccgtt cagcggcggc agcgcagcca ttgccatcag
    29941 cccgactaac atggcgatgg agataaccgg cattttcttg ccaataccac cgagtttttc
    30001 gatatcgcga tgaccggtac ggaaccagac gctccccgca ccaaggaaca gtacgctctt
    30061 gaacaggcta tggttaagca gatggtacag accaccaacc agaccaaggg caatcagcgc
    30121 cggttgttcg agcgcgatac ccgttacgcc agcgcccagc cccagcagga tgatgccgat
    30181 attttccagg gtgtggtaag ccagcagacg ttggatatta tgttccatca acgcatacag
    30241 accgccgacg aatgcggtga tcatgccgag taccagcagt gcgatccccc accacagcgg
    30301 tgcattaccg cccagtagtg acagagttaa aatgcccagc aggccaattt tcatgactac
    30361 cgtggaaaac agcgcggcag ctggcgcaga ggcgttggca tgtgcctgtg gcacccagcc
    30421 gtgcagcgga ataatcccgg ccagcaggcc aaagccaatc acaccgagca gccagatatc
    30481 ggaaccgagc ggcagctgtt gcatacgcat atccagcagg cgcagatcca gcgtgccgta
    30541 acgctgccac agcagccagc aggcaatcgc cagcagcaga gtgccaagac gccccagcgc
    30601 aaaccacagt ttgccctctt tgctgttgct ggtgaggaac accgcacaca gggccatgat
    30661 ttcggccatt accacgaaca tgccgaggtt gctggcgatg acggcgcaga cggcggcagc
    30721 catcaacata ttgatctgca agccgttgca cttcacctgc gcgtggcgat gccagtcaat
    30781 gttgtagagg ctgacaaaca gaccgcatag gccgagcgta atcagccaaa tcgcattaag
    30841 cggagagatt tgcacatcgt agcttaccag cgacagcgca ccgctcacgc caaccgcgcc
    30901 agtcagcact gtgaagcccg cggctgccgt atacaggcta ccaaccgcgc cgccaattcc
    30961 ggctatccag ccgcttagcg ccttttgaaa agaaaagaga aatgccagaa cagcggcggc
    31021 gacaaaccac gccacgccgc tattgatcag ggaaattgcg ctcatttagc ctctccactt
    31081 tgagcctgct gaaacaaggt gagatcgcca aaatccgtgt taaaagtcag ctcacgctta
    31141 cgtttgctga cgcgggcgat atcggtgtta tccaccagat gcagggcttt agtcgggcac
    31201 atccgcacgc aggccggacc ttgttcatca aagctacaaa ggtcacattt gacggcgatc
    31261 gcgcgaatac ctggcaccca gtcaagcaat gtgctgacac gcgccggagc aggcggtgcc
    31321 ggtggcgctt tcggggtatt ggcgtttgcc ggaatatcca gcggacggct gccggaaaat
    31381 tcaattgcgc caaacgggca ggcgatgccg cacagtttgc agcttacgca cagactttca
    31441 ttcaactgca cggccccatc aacgcgggtg atggcgttaa ccgggcaaac caccgcacag
    31501 ggcgcatctt cacagtggtg acagagctgc ggcgcagatt ctttttcatt gagcatcact
    31561 cgcaggcgcg gcattgattg caggccgtgc tggcgatgcg tctctgaaca ggcggcctca
    31621 caggtgtggc agccgataca gagcgtggag tcagcaatta caaaacgatt caccaggcat
    31681 tcctcaggtg attgtcattt ttgacgaaaa catgccgatg aaatgtcatt ttcgacactc
    31741 atcgacacgc ccatccccaa acaggcgtaa cgcctgcaaa acgggcaaag cctcagctca
    31801 tgctgccggg ctttgtccct ttaccagttg gcttaaattc accggcacat tgttttcaat
    31861 ggcggtgtat aagctgtcgt aaacgccagc gattttttcg agatagtgtt ccagaacctg
    31921 ttcgcgatcg cggatgttga ccttgccatc aatcatgccg tcaatgctca gttgcgcctg
    31981 cgcaatccgc tgtttgtctt caccatcttt tgtttcgacg taatactcga tggtgtggct
    32041 gttaaagcca tccgccaggg tgacgtaaat acgaaaatgt tcaaaaagga cgaaacagag
    32101 ttctttgtcc ggcgcgcagc tcatggcgtg atgcaggcgc gctttcgata acgtgattcc
    32161 ctgaaccagc gaattgcagt agatgtgcca ctggtcctgt aggcgacgat gccgctgtgc
    32221 gatgtaatcg gctttctcgc ttatttccca aatagtcatt gtcaggttac ccgtttaaca
    32281 gagatgccag ctttaagcat tttttgtgcc aacttttaat ttattgttat taaagcgatt
    32341 tttaagctaa agatgaattt cgtcgccgtg tcgacgtgtc atttcgacat catcgacatt
    32401 attcaccgca gggataatca acactggcac aattattgct tgtagctggc aatagttaat
    32461 gggaggcgat atgcacgaaa taaccctctg ccaacgggca ctggaattga tcgaacagca
    32521 ggccgcaaaa cacggcgcaa aacgcgtaac tggggtctgg ctcaaaattg gcgcattttc
    32581 ttgtgtcgaa accagctctc ttgccttttg ttttgatctg gtttgccgcg gcagcgtggc
    32641 ggaaggttgt aaactgcacc tcgaagaaca agaggccgaa tgctggtgtg aaacatgcca
    32701 acagtatgtg acgctactga cccagcgcgt ccgccgctgt ccacagtgtc atggtgacat
    32761 gctacagatt gtggcagacg acggtttaca gattcggcgg atagaaatag accaggagtg
    32821 agcgatgtgt acaacatgcg gttgcggtga aggcaacctc tatattgagg gtgatgaaca
    32881 taaccctcat tccgcgtttc gtagcgcgcc atttgccccg gcggcacgcc cgaagatgaa
    32941 aatcaccggc attaaagcgc ctgaatttac ccccagccag actgaagaag gcgacctgca
    33001 ttacggtcat ggcgaagcgg gcactcacgc gccgggcatg agccagcgtc ggatgctgga
    33061 agtcgaaatt gacgtgctgg acaaaaataa ccgtctggct gaacgcaacc gcgcgcgctt
    33121 tgctgcccgc aagcaactgg tgctcaacct ggtttccagc cctggttccg gtaaaaccac
    33181 cctgctgacg gaaactttaa tgcgcctgaa agacagcgtt ccgtgcgcgg ttattgaagg
    33241 tgaccagcaa accgtgaacg atgccgcacg cattcgcgct accggcaccc cagcgattca
    33301 ggtgaacacc ggtaaaggct gccatcttga cgcacagatg attgccgacg ccgcgccgcg
    33361 cctgccactg gacgataacg gtattctgtt tatcgaaaac gtcggtaacc tcgtttgccc
    33421 ggccagcttc gatctcggtg aaaaacacaa agtggcggtg ctttccgtta ccgaaggtga
    33481 agacaaaccg ctgaaatatc cgcacatgtt tgccgccgcc tcgctgatgc tgctcaacaa
    33541 agttgacttg ctgccgtatc tcaatttcga cgttgagaag tgcatcgcct gcgcccgtga
    33601 agtcaatcca gaaattgaaa tcatccttat ttccgccacc agcggcgaag ggatggacca
    33661 gtggctgaac tggctggaga cacagcgatg tgcataggcg ttcccggcca gatccgcact
    33721 attgacggca accaggcgaa agtcgacgtc tgcggcattc agcgcgatgt cgatttaacg
    33781 ttagtcggca gctgcgatga aaacggtcag ccacgcgtgg gccagtgggt actggttcac
    33841 gttggctttg ccatgagcgt aattaatgaa gccgaagcac gcgacactct cgacgcctta
    33901 caaaacatgt ttgacgttga gccagatgtc ggcgcgctgt tgtatggcga ggaaaaataa
    33961 tgcgttttgt tgatgaatat cgcgcgccgg aacaggtgat gcagttaatt gagcatctgc
    34021 gcgaacgtgc ttcacatctc tcttacaccg ccgaacgccc tctgcggatt atggaagtgt
    34081 gtggcggtca tacccacgcc atttttaaat tcggcctcga ccagttgcta ccggaaaacg
    34141 ttgagtttat ccacggtccg ggttgcccgg tgtgcgtact gccaatgggt agaatcgaca
    34201 cctgcgtgga gattgccagc catccggaag tcatcttctg tacctttggc gatgccatgc
    34261 gcgtaccagg gaaacagggg tcgctgttgc aggcaaaagc acgcggtgcc gatgtgcgca
    34321 tcgtttactc gccaatggat gcgttaaaac tggcgcagga gaatccaacc cgtaaagtgg
    34381 tattcttcgg cttaggtttt gaaaccacca tgccgaccac cgccatcact ctgcaacagg
    34441 cgaaagcccg cgatgtgcag aatttttact tcttctgcca gcacattacg cttatcccga
    34501 cgctgcgcag tttgctggaa cagccagata acggtatcga cgcgttcctc gcgccgggcc
    34561 acgtcagtat ggttatcggc actgatgcct ataattttat cgccagcgat tttcatcgcc
    34621 cgctggtggt cgctggtttc gaaccccttg atctactgca aggcgtggtg atgctggtgg
    34681 agcagaaaat agcggcccac agcaaggtag agaatcagta tcgtcgggtg gtaccggatg
    34741 ccggtaacct gctggcgcaa caggcgattg ccgatgtgtt ctgtgtcaac ggcgacagtg
    34801 aatggcgcgg cttaggcgtg attgaatctt ctggcgtgca cctgacgccg gattatcaac
    34861 gattcgatgc cgaaacacat ttccgcccgg caccgcagca ggtctgcgat gacccgcgtg
    34921 cgcgttgtgg cgaagtcttg acgggcaaat gtaagccgca tcaatgcccg ctgtttggta
    34981 acacctgtaa tcctcaaacc gcgtttggtg cgctgatggt ttcctccgaa ggagcgtgcg
    35041 ccgcgtggta tcagtatcgt cagcaggaga gtgaagcgtg aataatatcc aactcgccca
    35101 cggtagcggc ggccaggcga tgcagcaatt aatcaacagc ctgtttatgg aagcctttgc
    35161 caacccgtgg ctggcagagc aggaagatca ggcccgtctt gatttggcac agctggtagc
    35221 ggaaggcgac cgtctggcgt tctcaaccga cagttacgtc attgacccgc tgttctttcc
    35281 tggcggtaat atcggcaaac tggcgatttg cggcaccgcc aatgacgttg cggtcagtgg
    35341 cgcaattccg cgctatctct cctgtggctt tatcctcgaa gaaggattgc cgatggagac
    35401 actgaaagcc gtagtgacca gcatggcaga aaccgcccgc gcggcaggca ttgccatcgt
    35461 tactggcgat accaaagtgg tacagcgcgg cgcggcagat aaactgttta tcaacaccgc
    35521 tggcatgggc gcaattccgg cgaatattca ctggggcgca cagacgctaa ccgcaggcga
    35581 tgtattgctg gttagcggta cactcggcga ccatggggcg actatcctta acctgcgtga
    35641 gcaactaggg ctggatggag aactggtcag cgactgcgcg gtgctgacgc cgcttattca
    35701 gacgctgcgt gacattcccg gcgtgaaagc gctgcgtgat gccacccgtg gtggtgtaaa
    35761 cgcggtggtt catgagttcg cggcagcctg cggttgtggt attgaacttt cagaagcggc
    35821 actgccggtt aaacctgctg tgcgcggcgt ttgcgaactg ctgggactgg acgcccttaa
    35881 ctttgccaac gaaggcaaac tggtgatcgc cgttgaacgc aacgcagcag agcaagtgct
    35941 ggcggcgtta cattcccatc cattgggaaa agatgcggcg ctgattggcg aagtagtaga
    36001 acgtaaaggt gttcgtcttg ccggtctgta tggcgtgaaa cgaaccctcg atctaccgca
    36061 cgccgaaccg cttccgcgta tatgctaata aaattctaaa tctcctatag ttagtcaatg
    36121 accttttgca ccgctttgcg gtgctttcct ggaagaacaa aatgtcatat acaccgatga
    36181 gtgatctcgg acagcaaggg ttgttcgaca tcactcggac actattgcag cagcccgatc
    36241 tggcctcgct gtgtgaggct ctttcgcaac tggtaaagcg ttctgcgctc gccgacaacg
    36301 cggctattgt gttgtggcaa gcgcagactc aacgtgcgtc ttattacgca tcgcgtgaaa
    36361 aagacacccc cattaaatat gaagacgaaa ctgttctggc acacggtccg gtacgcagca
    36421 ttttgtcgcg ccctgatacg ctgcattgca gttacgaaga attttgtgaa acctggccgc
    36481 agctggccac aggtgggcta taccccaaat ttggtcacta ttgcctgatg ccactggcgg
    36541 cggaagggca tatttttggt ggctgtgaat ttattcgtca tgacgatcgc ccctggagcg
    36601 aaaaagagtt caatcgtctg caaacattta cgcagatcgt ttctgtcgtc accgaacaaa
    36661 tccagagccg cgtcgttaac aatgtcgact atgagttgtt atgccgggaa cgcgataact
    36721 tccgcatcct ggtcgccatc actaacgcgg tgctttcccg cctggatatg gacgaactgg
    36781 tcagcgaagt cgccaaagaa atccactact acttcgacat tgacgatatc agcatcgtct
    36841 tacgcagcca ccgtaaaaac aaactcaaca tctactccac tcactatctt gataaacagc
    36901 atcccgctca cgaacagagc gaagtcgatg aagccggaac cctcaccgaa cgcgtcttca
    36961 aaagtaaaga gatgctgttg attaatctcc acgagcggga cgatttagcc ccctatgaac
    37021 gcatgttgtt caacacctgg ggcaaccaga ttcaaacctt gtgcctgtta ccgctgatgt
    37081 ctggcgacac catgctgggc gtgctaaaac tggcgcaatg cgaagagaaa gtctttacca
    37141 ctaccaatct gagcttactg cgccagattg ccgaacgtgt ggcaatcgct gtcgataacg
    37201 ccctcgccta tcaggaaatt catcgtctga aagaacggct ggttgatgaa aacctcgccc
    37261 tgaccgagca gctcaacaat gttgatagtg aatttggcga gattattggc cgcagcgaag
    37321 ccatgtacag cgtacttaaa caagtggaaa tggtggcgca aagtgacagt accgttctga
    37381 tcctcggtga aactggtaca ggtaaagagc tgattgcccg cgcgatccat aatctcagtg
    37441 ggcgtaataa tcgccgtatg gtcaaaatga actgcgcggc gatgcctgcc ggattgctgg
    37501 aaagcgatct atttggtcat gagcgtgggg cttttaccgg tgccagcgct cagcgcattg
    37561 gtcgttttga actggcggat aaaagctcac tattcctcga tgaagtgggc gatatgccgc
    37621 tggagttaca gccgaagttg ctgcgtgtat tacaggaaca ggagtttgaa cgtctcggca
    37681 gcaacaaaat cattcagacg gacgtacgtt taattgccgc gactaaccgc gatctgaaaa
    37741 aaatggtcgc cgaccgagag ttccgtagcg atctctatta ccgtctgaac gtattcccga
    37801 tccacctgcc gccactacgc gagcgtccgg aagatattcc gctgctggcg aaagccttta
    37861 ccttcaaaat tgcccgtcgt ctggggcgca atatcgacag cattcctgcc gagacgctgc
    37921 gcatcctgag caacatggaa tggccgggca acgtgcgtga gctggaaaac gtcattgagc
    37981 gcgcggtatt gctaacacgc ggcaacgtgc tgcagctgtc attgccagat attgctttac
    38041 cagaacctga aacgccgcct gccgcaacgg ttgtcgccca ggagggcgaa gatgaatatc
    38101 agttgattgt tcgcgtgctg aaagaaacaa acggcgtggt tgccgggcct aaaggcgctg
    38161 cgcaacgtct ggggctgaaa cgcacgactc tgctgtcacg gatgaagcga ctgggaattg
    38221 ataaatcggc attgatttaa cagatgtggt tatactcgat caacattcaa tgaaaggagg
    38281 cagtatggca gtttcagcaa gaaatcaatt aaccggaaca gttagcacag tagcaatggg
    38341 cgctgtgaat gatgaagtag aactgacgct ggcaggtggt gcaaagctgg tggcaattgt
    38401 gactcacagc agccagcaag cgctgggtct ggcgaaaggg aaagaagcga ttgccctgat
    38461 taaagccccg tgggtcacgc tggcaacgga agactgtgga ctgaaattct ctgctcgcaa
    38521 ccagtttgct ggcagcgtgt cgacaatcac cgaaggcgca gtgaacgcca ctgtccatat
    38581 caaaacggat gcgggttttg aaattgtggc agtggtaaca aatgaaagtc aggatgaaat
    38641 gaaactcacc actggctcgc gcgttattgc attgatcaaa gcatcagcca tcctgattgc
    38701 caccaaagcg taaacgcctt atttgccgga gagtatggct ctccggcaga tttatttctt
    38761 tggacgatat ttttctggca actccggcac cggacgcttg tcatcgatga gatgacgcac
    38821 ggttaagatc ggatgacgcc acagcattct cggcccggcc caacgcataa tctgtttcat
    38881 ctcttcacgc tttgcaggct ggtaacagtg caccggacac tgcttacagg ctggtttctc
    38941 ttcaccgaac acacatttat ccagccgctt ttgcgcgtaa gcaaacaacg cctcgtaatg
    39001 ctccggctct gctgacgcct gcgggcattt cgcttgataa agatcgatca tttttttaat
    39061 cgtcagtttt tcacgagaga tacgcttgcc ggacatgctg cctccatact cttaaggtgc
    39121 atttatatta caacttaatt ttaaagggga ctatggctct gaaggaggag gtttcttcaa
    39181 ccgacgaatt tggcgatctt gtttcatcgt catcacgcaa aagctgcaaa gcagcatttt
    39241 tcgcggaacc gacatcaaga actcaccttt cgcgtcttcc cctgaaatga ttaactccgg
    39301 tatcatgtgc gccttatgtg attacaacga aaataaaaac catcacacta catttaacat
    39361 cagggagccg gacttaaccc catgagaaca atagaaaatt tcgacgccca tacgcctatg
    39421 atgcagcagt atctcaagct gaaagcccag catcccgaga tcctgctgtt ttaccggatg
    39481 ggcgactttt atgaactgtt ttatgacgac gcaaaacggg cgtcgcaact gctggatatt
    39541 tcactgacca aacgcggtgc ttcagcggga gagccaatcc cgatggcggg gattccctac
    39601 catgcggtgg aaaactacct cgccaaactg gtgaatcagg gcgaatcggt tgctatttgt
    39661 gaacagattg gcgatccggc gaccagcaaa gggccggtcg agcgcaaagt tgtccgtatc
    39721 gttacgccgg gcaccatcag cgatgaagcg ctgttgcagg aacgtcagga caacctgctg
    39781 gcggctatct ggcaggacag caaggggttc ggctacgcca cgctggatat cagctccggg
    39841 cgttttcgcc tgagcgaacc ggcggaccgc gaaacgatgg cggcagaact gcaacgtacg
    39901 aatccagcgg aattactgta tgcggaagat ttcgccgaga tgtcgctgat tgaaggtcgc
    39961 cgcggcctgc gtcgtcgccc gctgtgggag tttgaaatcg acaccgctcg ccagcaattg
    40021 aacctgcaat ttggcacccg agatctggtc ggttttggtg tggagaacgc accacgcgga
    40081 ctttgtgctg ccggttgtct gttgcagtat gcgaaagata cccaacgcac gaccctgccg
    40141 catattcgtt ctatcactat ggaacgtcag caggacagca tcattatgga tgccgcgacg
    40201 cgtcgtaacc tggaaattac tcagaacctg gccggcggtg cggaaaatac gctggcttca
    40261 gtgctcgact gtactgtaac gccgatgggt agtcgtatgc tgaaacgctg gctgcatatg
    40321 ccagtgcgcg atacccgcgt gttgcttgag cgccagcaaa ctattggcgc attgcaggat
    40381 ttcaccgccg agctgcagcc ggtactgcgt caggtcggcg acctggaacg tattctggcg
    40441 cgtctggctt tacgaactgc tcgtccacgc gatctggccc gtatgcgtca cgctttccag
    40501 caactgccgg agctgcgcgc gcaactggaa ggtgtcgata gcgccccggt acaagcactg
    40561 cgtgagaaga tgggcgagtt tgccgaactg cgcgacctgc tggagcgagc aatcatcgac
    40621 acgccgccgg tgctggtacg cgacggtggt gttattgcga ctggttataa cgaagaactg
    40681 gatgaatggc gcgcgctggc tgacggcgcg accgattatc tggagcgtct ggaagtccgc
    40741 gagcgtgaac gtactggcct ggacaccctg aaagtcggct ttaacgcggt tcatggttat
    40801 tacattcaga tcagccgtgg acaaagccat ctggcaccca tcaattacat gcgtcgccag
    40861 acgctgaaaa acgccgagcg ctacattatt ccagagctaa aagagtacga agacaaagtt
    40921 ctcacttcaa aaggcaaagc actggcactg gaaaaacagc tttatgaaga gttgttcgac
    40981 ctgctgttgc cgcatctgga agcgttgcaa cagagcgcga gcgcgctggc ggaactcgac
    41041 gtgctggtga acctggcgga acgggcctat acgctgaact acacctgccc gacctttatt
    41101 gataaaccgg gtattcgcat caccgaaggc cgccatccgg tggttgaaca ggtgctgaat
    41161 gagccgttta tcgccaaccc gctgaacctg tcgccgcagc gtcgcatgtt gatcattacc
    41221 ggtccgaata tgggcggtaa aagtacctat atgcgccaga ccgcactgat tgcgctgatg
    41281 gcctacatcg gcagctatgt accggcacaa aaagtcgaga ttggacctat cgatcgcatc
    41341 tttacccgcg taggggcagc ggatgatctg gcatccgggc gttcaacctt tatggtggaa
    41401 atgaccgaaa ccgccaatat tttacataac gccaccgagt acagtctggt gctgatggat
    41461 gagattgggc gtggaacgtc cacttacgat ggtctgtcgc tggcgtgggc gtgcgcggaa
    41521 aatctggcga ataagattaa agcgttgacg ttgtttgcta cccactattt cgagctgacc
    41581 cagttaccgg agaaaatgga aggcgtcgcc aacgtgcatc tcgatgcact ggagcacggc
    41641 gacaccattg cctttatgca cagcgtgcag gatggcgcag cgagcaaaag ctacggcctg
    41701 gcggttgcag ctctggccgg cgtgccaaaa gaggttatta agcgcgcacg gcaaaaactg
    41761 cgtgagctgg aaagcatttc gccgaacgcc gccgctacgc aagtggatgg tacgcaaatg
    41821 tctttgctgt cagtgccaga agaaacgtcg cctgcggtcg aagcactgga aaatctcgac
    41881 ccggattcac tgactccgcg tcaggcgctg gagtggattt atcgcttgaa gagtctggtg
    41941 taataacaat tcccgatagt cttttgctat cgggaatatt aacgacaact gacgaatcaa
    42001 ataaaaatac cctgtataat aggaaatctt attttacagg gtaaaaccat gccatctaca
    42061 cgctaccaaa aaatcaatgc ccatcactat caccatatat gggccgttgg tgatattcat
    42121 ggtgactatc aattattaca atcccgctta catcaactct ctttttgtcc tgaaaccgac
    42181 ttgcttattt ctgtcggcga taacatcgat cgtgggcccg agagtcttaa cgtgctgcgc
    42241 ctgttaaacc agccctggtt tatctccgtc aaaggcaacc acgaagcgat ggcgcttgag
    42301 gcattcgaaa ctggcgatgg aaatatgtgg cttgccagcg gtggtgactg gtttttcgat
    42361 ttaaatgatt cagagcaaca agaggcaata gatctgttgc tgaaattcca tcaccttcca
    42421 catattattg aaatcactaa cgacaccata aaatatgtca tcgcacatgc agattatccg
    42481 gggaaggagt atcagtttgg caaagaaata gcggagagcg aattactctg gcctgttgat
    42541 cgtgtgcaga aatcacttaa tagcgaatta caaaaaataa acggcgctga ttattttata
    42601 tttggacata tgatgtttga taacattcag acgttcgcaa accagattta tattgatacc
    42661 ggatcgccga aaagcgggcg gctgtcattt tataaaataa agtagtctca tgcttcttct
    42721 gtgaagcatg agtaacccgg cgttattgca ggtcattatt catttttcgc taccagcaaa
    42781 gagagatcca gcttcgccag cgcgcggctg gcactctccg gcagaccatc gtcagtaatg
    42841 atctgatcaa actcgcttaa tggtaacgcc agccatgtcg ccacctgtcc atatttcgtc
    42901 gcatcacaga ccaaaactcg ctggcggctg gcactggcaa tcgcccgttt caccgtgact
    42961 ttatcttctg ctggcgtaga gatcccccgc acactccatg acgatgcaga aataaaagcc
    43021 tgatcaatca tcaggctacg cagcatggtc gcagcggctt ccccaacaca ggaacggttt
    43081 tcccgacaca ctgcgccgcc agtgtgaata attgtgcaat tactgttgtc gagcaagtag
    43141 tccgcaataa cgaagtcgtt agtaaccact gtgagtggct ccatgtgagt cagatgctgt
    43201 gcaatcgcta acgtggtcgt tcccgcatcc agatagatac aacgtcccgg ctgaacaaga
    43261 cttgccgcca gcttgccaat agccgctttt tgcgtcattg ccagcgcagt ttttacctga
    43321 tgagaaggtt catgcgccac ccgtcccgga gactggacgc ccccggacac caacacaacg
    43381 gctccctgct gctccagttt ttgtaaatcc cgacgaatgg tcatatgtga cacattcatt
    43441 ctgtccgtta gttcagcaat actaacaatg cctttttcag ctaccatctc aaggatgatt
    43501 tggcgacgct ctacgggtat caacttttgc tccttccttt gtcctgctga cattctacgc
    43561 tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag ttcacataaa ataacataca
    43621 atgttaattt ttgtgaatca gctcaccata tcgttatctt tccgcgctta tattcacaac
    43681 atcaaacaaa atatcactta aattaacaag gagagcagat gaaaacggga tcggagtttc
    43741 atgtcggtat cgttggctta gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg
    43801 caggtctttc tacctggggc gcagacctga acagcaatgc ctgcgctacg ttgaaagagg
    43861 caggtgcttg cggagtttct gataacgccg cgacgtttgc cgaaaaactg gacgctctgc
    43921 tggtgctggt ggtcaatgcg gcccaggtta aacaggtgct gtttggtgaa acaggcgttg
    43981 cacaacatct gaaacccggt acggcagtaa tggtttcttc cactatcgcc agtgctgatg
    44041 cgcaagaaat tgctaccgct ctggctggat tcgatctgga aatgctggat gcgccagttt
    44101 ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt catggcctcc ggtagcgata
    44161 ttgcctttga acgactagca cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag
    44221 gtgcagaacc gggactaggt tcgaccgtaa aaattattca ccagttgtta gcgggcgtac
    44281 atattgctgc cggagccgaa gcgatggcac ttgcagcccg tgcggggatc ccgctggatg
    44341 tgatgtatga cgtcgtgacc aatgccgccg gaaattcatg gatgttcgaa aaccggatgc
    44401 gtcatgtggt ggatggcgat tacaccccgc attcagccgt cgatattttt gttaaggatc
    44461 ttggtctggt tgccgatacc gccaaagccc tgcacttccc gctgccattg gcctcaacag
    44521 cattgaatat gttcaccagc gccagtaacg cgggttacgg gaaagaagac gatagcgcag
    44581 ttatcaagat tttctctggc atcaccctac cgggagcgaa atcatgatca agattggcgt
    44641 tatcgccgat gattttaccg gcgcgacgga tatcgccagt tttctggtgg aaaacggtct
    44701 accaacggta caaattaacg gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact
    44761 ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa gccacacagc aatcgctggc
    44821 ggctctgagc tggttgcaac agcaaggttg caaacagatc tatttcaaat actgctctac
    44881 tttcgacagt tcggcgaaag gtaatatcgg cccggttacc gatgcattaa tggatgctct
    44941 cgacacgccg tttacggtct tctctccggc cctgccggtc aacggacgta cggtttatca
    45001 ggggtatttg ttcgtaatga atcaactgct ggccgaatcc gggatgcgcc atcacccggt
    45061 aaatcccatg accgacagct atcttccccg tctggttgaa gcgcaatcca cagggcgctg
    45121 cggcgtcgtt tcggcacatg ttttcgaaca aggtgtggat gccgttcgtc aggagctggc
    45181 tcgcttacag caagagggct accgctacgc ggtgcttgat gcgctgaccg aacaccatct
    45241 ggaaattcag ggagaagcct tgcgcgatac cccactggta acgggcggtt ctggtctggc
    45301 gattggcctt gcccggcagt gggcgcaaga aaacggtaac caggctcgcg aagcagggcg
    45361 tccgctcgct gggcgcggcg tagtgctctc cggttcatgc tctcaaatga ccaaccgcca
    45421 ggtggcacat taccgtcaaa ttgcaccagc ccgtgaggtt gatgtggcac gctgcctctc
    45481 aaccgaaact ctggccgctt atgcacacga gctggcagag tgggttctgg gccaggaaag
    45541 tgtacttgct ccactggttt ttgccaccgc cagcactgac gcattggcag caattcaaca
    45601 gcaatacggt gcacaaaaag ccagtcaggc agtggaaaca ctgttttctc aactagcggc
    45661 gcggttagca gcggaaggcg tgacacgctt tattgtcgca ggcggtgaga cctccggcgt
    45721 agtcacacag agcctgggca taaaaggttt tcatattggc ccaaccattt cccccggcgt
    45781 gccgtgggta aacgcactgg ataagcctgt ctcactcgcc cttaaatctg gcaacttcgg
    45841 tgatgaagcc tttttttcac gagcccaaag agagttttta tcatgagcga tttcgcaaaa
    45901 gtagagcagt ctttgcgaga ggagatgacg cggattgcca gttcattctt tcagcgcgga
    45961 tatgcaactg gttcggctgg caatctgtcg ctgcttttac ctgacgggaa tttactggcg
    46021 acaccgacag gttcatgcct gggcaatctc gatccgcagc ggctttccaa agtcaccgcg
    46081 gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg tgctctttca tctggcgctg
    46141 tatcgcaaca atccgcgctg taaagcggta gtgcatttgc acagtacgtg gtcgacggcg
    46201 ctttcctgcc tggaagggct ggacagcaat aacgttattc ggccgttcac accatacgtg
    46261 gtgatgcgga tgggaaacgt cccgctggtg ccctattacc gaccgggcga taaacgcatc
    46321 gcacaggatc tggcggaact ggcagcagac aatcaggctt ttttactggc aaatcatggc
    46381 ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca atatggaaga gctagaggaa
    46441 acggcaaagc tgatttttat tctcggtgac cgcccgatcc gttatctgac cgcaggtgaa
    46501 attgcggaat taaggagtta atgcaatgcc tcgttttgca gctaatttat ccatgatgtt
    46561 caccgaagtg ccttttattg aacgcttcgc cgcagcgcga aaagccgggt tcgatgctgt
    46621 ggagtttctg tttccctatg actactcccc ccagcaaatc cagcagcaac tggcgcaaaa
    46681 ccacctgacg ctggcgctgt tcaataccgc tcccggagat attaatgcgg gagaatgggg
    46741 attatccgcc cttcccggac gtgagcacga agcacgtgca gacatcgacc tggcactgga
    46801 atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg gcaggcgtcg tgcccgctgg
    46861 cgaagatgct gaacggtacc gggcagtatt tattgataat ctccgctacg ctgccgacca
    46921 ctttgcgcca cacggcaagc gaatattggt tgaagcactc agccccggcg ttaagcctca
    46981 ttatctcttc tccagccagt atcaggcact ggctattgtt gaagaggttg cgcgagataa
    47041 cgtttttatt caactagata ctttccatgc gcaaaaagta gatggcaacc tgacgcattt
    47101 aatccgcgac tacgccggga aatatgcgca cgtacaaatt gccggactac ctgatcgaca
    47161 tgaaccggac gatggagaaa tcaactatcc gtggctgttc cgcctgttcg atgaagtggg
    47221 atatcagggc tggatcggtt gtgaatacaa acctcgtggc ctcaccgaag aaggacttgg
    47281 ctggtttgac gcctggcgct aattcgttaa atccctaatt acaacgtacc catacatccc
    47341 cccatctacg gatggggtag gttaactata actttcagac agggtttccc atgtccacaa
    47401 ttacattgtt atgcattgcg ttaactggcg taatcatgct gttgctgctg gtcatcaagg
    47461 caaaggtaca accattcgtt gctctgctcc tcgtcagcct gttagtcgca cttgcggcgg
    47521 gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg gatgggcggt gtgcttggtt
    47581 ccgtcactat tattattggt ctgggcgcta tgctggggcg tatgatcgaa cactctggtg
    47641 gtgcagagtc actggctaat tatttcagtc gcaagttagg cgacaaacga actatcgctg
    47701 cgctgactct ggcagcgttc ttcctcggta ttcccgtctt ctttgatgtc ggctttatta
    47761 ttcttgcgcc aatcatttac ggttttgcca aggttgccaa aatatcacca ctcaaatttg
    47821 gtctgcctgt cgctgggatc atgctcactg ttcacgtggc ggtaccgcct catccaggcc
    47881 ctgtcgccgc agcggggtta ctccacgcag acatcggctg gctaaccatc atcggtattg
    47941 cgatttctat tcccgtaggg attgttggct actttgcagc gaaaataatc aataagcgcc
    48001 aatatgcgat gtcggtcgag gtactggaac agatgcaact ggctccggcc agtgaggaag
    48061 gcgcgacaaa attaagcgat aaaataaatc caccgggcgt cgcgctggtc acctcgctaa
    48121 ttgttattcc tatcgcgatt atcatggcgg gtacggtttc cgcaacactg atgccgcctt
    48181 cgcatcccct gcttggtacg ctacagctga tcggctcacc aatggtagct ctaatgattg
    48241 cgctggtgct ggcattctgg ttattggctt tacgtcgcgg ctggagctta caacacacca
    48301 gcgacattat gggctcagcc cttcccactg cggcggtagt gattttggtt actggtgctg
    48361 gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg taaagccctt gccaacatgt
    48421 tacaaatgat tgacctgcct ctgttaccag ccgcatttat tatttcactg gcgctgcgtg
    48481 catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg cgggttactc tcagaagcgg
    48541 tgatgggatt gaatccaatt cagtgcgtat tggtgacgct ggcagcctgc tttggtgggc
    48601 ttggcgcgtc acatattaat gactcagggt tctggattgt gaccaaatat ctggggttgt
    48661 cggtagcaga cgggctgaaa acctggacag tgttaacgac cattctcggt tttaccggat
    48721 tcttaattac ctggtgcata tgggcggtaa tttgacctta ataaaaggtc cgatgggcat
    48781 cggacctttt atttagcgct taccttccgc cagcagcggc gggatgctcg gcaccatcgg
    48841 cgcgtcatca atatctttct gcgtcatgcg gaacgcttcg ggataatgtt cgcggctggt
    48901 gcggcgtagc ggttcggtat cgcgccaggt atagaggcaa tgctggcact ggtagaccat
    48961 ccagacatct tttaccggcg atttcgccat cacttcaatc tgttcatcgg cacaacgtgg
    49021 acaaatcatc ttgttctcct tattgacgtg cggccagcat agcggtcagt ttttcagccc
    49081 aggctttggt ttccggtaag tccaccaccg gctggctgta gtgaccacgg ttgtccgggg
    49141 cgacaggcgt ggtggcgtcg ataatcagct tgtcggtgat ccccgccgga cttgagccag
    49201 ggtcgagttc cagcacggac atattcggca actgtaccaa atcccctgcc gggttcactt
    49261 tcgaggagag cgcccacatc acctgcggca ggttgaacgg atcaacgtct tcatcgacca
    49321 taatcaccat cttcacgtag cccagaccgt gcggcgtggt catcgcacgc aggcccaccg
    49381 cgcgggcaaa gccgccgtag cgttttttgg tggagataat cgccagcagg ccgtgggtgt
    49441 acatggcgtt taccgcctgc acttccggga actcggcttt cagttgttga tacagcggca
    49501 cgcaggtggc aggccccatc aggtagtcga tttcggtcca cggcatacca agatagagcg
    49561 attcgaaaat cggtttggta cggtaagaga ctttatcgat acgcaccacg gtcatgttac
    49621 gcccgccgga gtagtggccg gtaaactcac cgaacggccc ttcaatctca cgtttacgac
    49681 tttcgataac cccttcgagg atcacttccg agccccacgg cacatcaaaa ccggtcagcg
    49741 gggcggtggc gatcgggtac gggctttcgc gcagcgcgcc tgccatttcg tactcagact
    49801 gatcgtattt cagcggcgtg gcccccatta gggtgatgat cggatcgtta ccgagtgtga
    49861 tggcaatcgg cagatcttca ccgcgctctt ctgctttatg cagatgcagg gcgatatcgt
    49921 gcatcggcac cggttgcagg ccgagcttac gcttgccctt cacttccatg cggtagatgc
    49981 cgacgttctg cttgccgaag ttatccgggt cgagcggatc gcgggaaacc acgcacgctt
    50041 tatcgagata gaaaccgcca tcaccgtcgt ttaaacgaaa cagcggcaga atattgaaca
    50101 ggttgatctc gtcgccatcg acggtgttct gcgcccaggc tggattggcg cggcgctccg
    50161 gggcaatggg gaagttatcc cagcggcgga taaactcatc aatctgtttt ttaaccgggg
    50221 tgtttggcgg caggccgagg gaaatcgcgt ggttctgcca ggaaccgatg gtgttcattg
    50281 ccacgcgggc atcgataaag ccgcgaatat tatcaaacca aagcgcgggc gcgccgtcgc
    50341 cgatacgccc ggtggcgttg gctgcggctg ccagatccgg ttccgcgttc acctcttcgc
    50401 tgattttcag taactggccg tggtcatcaa gcgcctgtaa aaagctgcgt aaatcatcaa
    50461 atgccattat tcattctcct gagaaaaatt ccgggcctgc ggcaatcctt gccagcgcct
    50521 ggcgtgagga tgttcgaggc caaattgatc cagcacgcgg gctaccacgt ggtggacaat
    50581 gtcatctacc gtttcgggat ggttataaaa ggcaggcatc ggcggcacca tcgccacgcc
    50641 catgcgcgaa agtgcgagca tattttcgag atggattgtg ctaagcggca tttcacgcgg
    50701 caccagcacc agtttgcggc cttctttgag cacgacgtcc gccgcgcgcc ccaccaggcc
    50761 atcggcgtaa ccagcgcgga taccggcgag cgttttcata ctgcatggaa taacgatcat
    50821 gccgtcggta cgaaaggagc cggaagagat agtcgccgcc tgatccgccg ggttatggct
    50881 gaagtctgcg agggcggcaa catcgcgagc gctgtaaggc gtttccagtt caatcgtggt
    50941 tttcgcccac ttcgacatca ctagatgagt ctcgacattc ggcatctccc gcagcgcttg
    51001 cagtaatgcc acaccaagag gcgcaccggt agcccctgtc atcccgacga tcagtttcat
    51061 tgcttactct cttaattgtt cgtatacgaa cgttattatt aacatactcc ttcatttcca
    51121 cagcggcaag gattgaaaag taacaacatc ttcttcgtca ctgaaaaacc gctatgatag
    51181 cgacagagaa ttagcccgga gtttacatgg cgttacgaaa taaagcgttc catcagttac
    51241 gacagctttt tcagcagcat acagctcgct ggcagcacga gttacctgac ctgaccaaac
    51301 cacagtacgc ggtgatgcgc gccattgctg ataagcctgg tatcgaacag gtggcactga
    51361 tagaagcagc agtcagcacc aaagcaacgc tggcagaaat gttggcaaga atggaaaatc
    51421 gtggcttagt cagacgtgaa catgacgccg ctgacaagcg gcgacgcttt gtctggctga
    51481 ctgccgaagg agaaaaggta cttgctgcgg cgatccccat tggtgacagt gtggatgagg
    51541 aatttttggg gcgtttgagt gcagaagagc aggagctgtt tatgcagttg gtgcgcaaga
    51601 tgatgagcaa ataggatgct gattgccggg taggacgctg acgagtctta tccaggcgac
    51661 aaaacacacc gacttcccaa acaaaagaaa aaggccagcc tcgcttgaga ctggcctttc
    51721 tgacagatgc ttacttattc gcggaacagc gcttcgatat tcagcccctg cgtttgcagg
    51781 atttcgcgca aacggcgcag gccttcaacc tgaatctggc gaacacgttc acgggtgagg
    51841 ccaatttcac gacctacatc ttccagtgtt gccgcttcgt accccagcaa accgaatcga
    51901 cgtgccagta cttcacgctg tttggcgttc agctcgaaca gccatttgac gatgctctgc
    51961 ttcatatcgt catcttgcgt ggtatcttcc ggaccgttct ctttttcatc ggccaggatg
    52021 tccagcaacg ctttttcgga atcaccaccc agcggggtgt ctaccgaggt aatgcgctcg
    52081 ttaagacgaa gcatacggct gacgtcatca actggcttat ccagttgctc tgcgatctct
    52141 tccgcacttg gttcgtggtc cagtttatgg gacaactcgc gtgcggttcg caggtaaacg
    52201 ttcagctcct ttacgatgtg aataggcaaa cgaatagtac gggtttggtt cataatcgcc
    52261 cgttcgatcg tctggcgaat ccaccaggtt gcgtatgttg agaagcggaa accacgttcc
    52321 gggtcaaact tctctaccgc gcggatcagc cccaggttgc cctcttcgat caggtccagc
    52381 aacgccagac cacgattgcc ataacggcgg gcaattttta ccaccagacg caagttactc
    52441 tcgatcatcc ggcggcggga ggcaacatct ccacgcagtg cgcgacgcgc aaaataaact
    52501 tcttcttcgg ccgttaacag tggtgaataa ccaatctcac caaggtaaag ctgagtcgcg
    52561 tccagcacac gctgtgtggc tccctgcgat aacagttcct cttcggccaa atcgttatca
    52621 ctgggttcct cttctactaa ggccttttcg tcaaaaacct caactccgtt ctcatcaaat
    52681 tccgcatctt catttaaatc atgaactttc agcgtattct gactcataag gtggctccta
    52741 cccgtgatcc cttgacggaa cattcaagca aaagcctggt tccgccgatt tatcgctgcg
    52801 gcaaataacg cagcgggttt acggatttcc ccttgtaacg aatttcaaaa tgcaagcgtg
    52861 ttgaactggt tccggtgcta cccatggttg ctattttttg ccccgccttc acttcttgtt
    52921 gttcccggac cagcattgtg tcgttatggg cgtaggcact caggtaatca tcattatgtt
    52981 tgatgataat cagattaccg tagccgcgca gcgcgttacc ggcataaaca acgcggccat
    53041 ctgcggtcgc gataattgcc tgtcctttgc tgcctgcgat atcaatcccc ttgttgcccc
    53101 cctcagaagc gccaaaggtt tcgatcactt tgccctcagt cggccagcgc caggtggaga
    53161 taggcgtact ggttgatgta ctgctgacag tcggctcggt tgtgcttgct gttggtaccg
    53221 ttacaggcgc tgtgaccgtg gtcgcagttg gcttgttgtt cggcaacatt ttgttagcac
    53281 tctgttcacc cgaagactca gaatacgtaa ttgtcggttg cgacgcaaca gcaacggtgg
    53341 aattttgtgc aggcttgatc acaactcctt gctctgctgc gtcggcctgg gtaatggcat
    53401 ttccgccagt gattggcgta ccggaagcat tacccacctg taaggtctga ccgacgttca
    53461 gcgcgtatgg tgcctgaata ttgttgcgct gagcaaggtc acggaaatcg ttgccagtaa
    53521 tccaggcgat atagaaaagt gtgtcgcctt ttttcacggt ataggtactg ccgctataac
    53581 tgcctttcgg aatgttccca tactgacggt tgtagacgat gcgtccgttt tccatctgta
    53641 ccggctgctg agctactggc tgtactggct ggatttgcgg ttgttgagta gcctgaattt
    53701 gtggctgctg taccggctga atttgcggtt gctgcgctgt agacgtcgtc cccattttcg
    53761 gcggcggcgt aatcaacata ccagaattgg tatttgcagg cgcattgcca ttaacggagc
    53821 tgaccggtgc cggtggattt gaagtgtcag aacagcctgc cagccatagc gaaaccagtg
    53881 acaaagccgc aatgcggcga acggtgaatt ttgggcttcc cgcgctcatt tatcccccag
    53941 gaaaaattgg ttaataacca gtgacataat taccgtgcaa ggcaccatac tgaacactgg
    54001 aaaagatgtt cacgatacgc tgacctgcgg caaaataacc aggaaaaatc caggtatttc
    54061 ctcacgtttt aagccagctc acccttcact aaagggacaa agcgcacggc ctccacggta
    54121 tcgataataa attcgcctcc ccgacgacgc acccgtttca aatactggtg ctcctcccct
    54181 acgggtaaga cgagaattcc gccttcgtcc agctgcgtca ttagcgcagt tggaatttcc
    54241 ggcggtgccg ccgtaacaat gatagcgtca aacggcgcac gtgcctgcca accttgccat
    54301 ccatcgccat gacgggttga aacattatgt aaatcaagat ttttcaggcg gcgacgcgcc
    54361 tgccactgca agcctttaat ccgttcaacc gagcaaacat gctggacaag atgcgccagg
    54421 attgccgttt gatatcccga accggtgcca atttccagca cccgcgactg cggcgtcagt
    54481 tcgagtaatt cggtcattcg cgccaccata tacggctgcg aaattgtctg cccctgacct
    54541 atcggcaagg cgatattgtc ccaggctttt tgttcaaacg cttcatcaac gaatttttca
    54601 cgcggcacgg cggcaagtgc attcagcacc agctcatcct gaataccttg cgcacgtaat
    54661 tgatccagaa gtgcttgtac gcgtctgctt accattgcgt gccaactccc acgctgttta
    54721 accagtctga aaccacatct tgcgcgctat gcgcagttaa atccacatgc agcggcgtga
    54781 tggaaacata gccctcatct accgcagcaa aatcggtccc cggaccggca tcacatttac
    54841 cgcccggcgg gccaatccag tacagcgtat tgccgcgcgg atcttgctgc gggatcacct
    54901 gatctgccgg atgtcgtgta ccgcagcgcg tcacgcgaat acctttgatt tgatccaatg
    54961 gtaaatccgg aacgttaata ttaagaatac gtccggtgcg cagcggctct ttacacagtg
    55021 cgcgcaaaat tgaacaggtt accgccgcgg cagtgtcgta atgtttatgc ccgtcaagcg
    55081 agacggccag cgccggaaaa cctaaatgac ggccttccat cgcggcggct accgtaccgg
    55141 aataaataac atcatccccc agattcggcc cggcgttaat tccggacaca acaatgtccg
    55201 ggcgcggacg catcagagca ttcacgccaa gatagacgca atcggtcggg gttcccattt
    55261 gcacagcaat atcaccattt tcaaaggtaa acgtgcgcag ggaggattcc agtgtcagag
    55321 aatttgaagc gccgctgcgg ttacgatcgg gggcgaccac ctgaacgtca gcaaactcac
    55381 gcaaggcttt cgccagcgtt tgtataccgg gtgcatgtac cccgtcatca ttactcagca
    55441 atatgcgcat aatcacctgt tgtgttgata agttccctga caacgctggt tgcaaaactt
    55501 cccgccggaa gccagaaacg gatttctacg gtgacgtcat cccaccagtt ccagcttaat
    55561 ttttgcggat acagcagcat cgctctgcgc gcggcttcaa ctttttcacg caccagtaaa
    55621 gtttgtaatt cagtttcttc ggcgacagct gcttgttcga atgccagcgc ttcacgctga
    55681 gttccccatt cgccactgcc cggcaatgcg gcggttatca tcagctcttt atcgttgacg
    55741 cgacgctgta attccgccag ttcttcggtg gttgcaacaa accagctacc acgtccggct
    55801 aattgtagcg catcgccgtc aacaacttga ttaacgtctg cttttttgag gcgctcagca
    55861 acaatctgat taaacaacgc actgcgggct gccgacaacc aaaaactccg tttattgcga
    55921 tcgcgcaccg gagtattggt ttgcgcccaa cgcagcgcgc cctgcaagtt gctaccgcca
    55981 atcccaaaac gttgggcacc gaagtagttc ggtacacctt ttacgcaaat atcgatcaga
    56041 cgttgttcaa cgtcatcgcg attgctcact tcgcgtagta ccagggtaaa ggcgttacct
    56101 ttcagcgccc ctaaacgcag cttgcgcttg tgccgcgcat actccagcac ctggcagcct
    56161 tccagttgaa aggcgctcag atcgggcatc tctttgcccg gcacgcgagc gcataaccac
    56221 tgttccgtaa cagcatgttt atctttttgc ccagcgaagc tgacttcacg ggcatgaatt
    56281 ttcaggaatt tcgccagtgc atccgccaca aaacgggtat tgcagccgtt tttgaggatt
    56341 ctaaccagaa tatgctcacc ttcaccatca ggctcaaagc ccaaatcttc caccaccaca
    56401 aagtcttccg gattggcttt cagcagcccg gtgccttgcg gtttaccgtg gaggtaagtg
    56461 agattatcaa actcaatcat tttgttgcct taatgagtag cgccaccgct tcacaggcaa
    56521 tcccttcccc acgtccggta aatcccagtt tttccgtagt agtggctttc acgttaacat
    56581 catccatatg gcagccgaga tcttcggcaa taaacacgcg catttgtgga atgtgcggca
    56641 acatcttcgg tgcctgagcg atgatagtga catcgacgtt gccgagggca taacctttcg
    56701 cctgaatacg acgccaggct tcgcgcaaca gctcgcggct gtcggcacct ttaaatgccg
    56761 gatcggtatc cgggaacagc ttgcctatat ctcccagcgc cgccgcgcca agcaatgcat
    56821 cggtcaacgc atggagcgcc acgtcgccat cagaatgcgc cagcagccct ttttcgtaag
    56881 gaatgcgtac gccaccaatg ataattgggc cttcaccgcc aaaggcgtgt acgtcaaaac
    56941 cgtgtccaat tcgcattatg tattctcctg atggatggtt cgggtgaggt aaaattcggc
    57001 cagcgccaaa tcctccgggc gcgttacctt gatgttatcc gcgcggcctt caacaagttg
    57061 cggatgaaaa ccgcaatatt ccagcgccga ggcctcatcg gtaatggtag cgccttcatt
    57121 tagagcgcgc gtcaggcagt catgtaacag ctcacgaggg aaaaattgcg gcgtcagcgc
    57181 gtgccataag ccgttgcgat caacggtatg agcaatggca tttttgcccg gttcggcacg
    57241 tttcatcgta tcgcgcaccg gcgcagcgag gatccccccc gtgcggctgg tttcgctcaa
    57301 cgccaacaat cgcgcgaggt catcctgatg cagacaagga cgagcggcgt catgcaccaa
    57361 tacccactgc gcgtcgccag cggctttcag acctgccagc acggaatcgg cacgctcttc
    57421 accaccatca acaaccgtga ttcgtggatg attcgccaga ggaagttgtg caaagcggct
    57481 atcgccagga cttatggcaa tgacgacacg tttcaccctg ggatgcgcca gcagcgcata
    57541 caccgagtgt tcaagaatgg tttgattacc gattgagaga tattgcttag gacattccgt
    57601 ttgcattcgg cggccaaatc cggccgccgg aaccacggcg caaacatcca aatgagtggt
    57661 tgccatgtta attcccgggc tgatttatcg attattttgc cccgcagact gtgcgcgctt
    57721 tgacgcgtca ggcaccagac gataaaacgt ttcgcccggc ctggtcatgc tgagttcatt
    57781 acgcgcacgc tcttcgagcg cctcctggcc gccattgaga tcgtcaattt cggcaaaaag
    57841 ctgatcgttt cgcgctttaa gtttcgcgtt tgtagcttgc tgtgccgcca catcattatt
    57901 gacgcgagta tagtcatgta taccgttctt accgaaccac agcgaatact gtagccagac
    57961 cagaatagcc agcaacagca gcgttagttt acccatcctg ccccctgaaa aacggcatct
    58021 tcatcccatg catccgaaga cgactctaca tcctctgttg gggataccgc gacaacgcgg
    58081 gcaaatgtac cacatttgtc cattgttacg tatacccagg gcgtgcagaa cataatctca
    58141 ttattagtta cggattgaat tatgaacaga atagagagga aagaacaatt aacccagtag
    58201 ccacataaac agcgcaccaa acataatgcc aaccgtcgcc agagtgaaaa caatactgta
    58261 gcgtagcttt ccgtccatca atgaatgcag tgcaatcccc accactacag cgacgggcat
    58321 cagcgccaga aagaaaggcc aggtgtagat aaaaaagagc agcgtgttag agccataaat
    58381 caacatcgga attgccaacg cgcacagcca cgataaaaaa ccaaccacag cgcctggcag
    58441 cgaccatgtg gtgtcttcaa cctcagtaag gctgtcgtta tttgttaatg taatgttgtg
    58501 gctgttacgc atatctgatc ctgttacttt gacgaaccgg gcatggaaac ccggcggtgt
    58561 ctcaggatct gataatatcg ttctgtctca acagatctaa taattgctgt accaaatttg
    58621 ttactaattg ttcaccattg agatgaattt ctgccgattc aggcgcttcg taaacggaat
    58681 ctattcccgt aaagttgcgc agttcaccgg cacgcgcttt cttatataaa ccttttggat
    58741 cgcgggcttc gcaaatcgcc agcggcgtat cgacaaacac ttcgataaag cgcccttctc
    58801 ctacgcgttc gcgaaccatc tggcgttcgg cgcggtgtgg cgagataaat gcggtcagca
    58861 ccaccagtcc ggcttcaacc atcaaattcg ccacttcacc aacccgacgg atattctctt
    58921 tacggtcggc atcgctaaaa ccgagatcgc tgcataatcc gtggcgaacg ttgtcgccat
    58981 ccagcagata cgtactgacg ccaagtttat gtaacgcctc ctccagcgcc cccgcgacgg
    59041 ttgatttacc ggaaccagag aggccggtaa accacagcac aacaccacga tgaccgtggt
    59101 gctgttcgcg ttgttgcgga gttactgggt ggctatgcca gacgacgttt tcgtcatgca
    59161 gcgccattat ttatctccca gtaaatcgcg cgcaccccaa tgcgggaaat ggcggcgaac
    59221 cagggcattc aattccagtt cgaatgcact gaattcagat ggcgcagcag ttgcctggct
    59281 aactggttcg tgcaccatac cggcacctac ggtcacatta ctcaggcgat cgataaaaat
    59341 cagcccaccg gtaaccgggt tttgctgata acgatctaac accagtggct cgtcaaaggt
    59401 gagatctacc aggccgatgc cgttcagcgg caggttttca acttcgcgtt gggtaaggtt
    59461 attgatatca acctgataac gaatgccatc aacacgagca cgcgtcttct taccggcaat
    59521 tttgatgtca taactctggc ccggggaaag cggctgttcc gccatccata ccacatccac
    59581 cgacgcgctc tgcacagccg gtaacgcttc gtctgccgcc agcagcagat cgccacggct
    59641 gatgtcgatc tcatccgtca gcaccagggt gatcgcttct ccggcaaagg cttcttcgcg
    59701 atcgccatca aaagtcacga tccgcgcgac gtttgattcc acaccagagg gcagcacttt
    59761 gacacgttgc ccgacttcca cgcgaccgga tgccagcgtt ccggcgtagc cacgaaaatc
    59821 gagattcgga cggttaacgt actgcaccgg gaagcgcatt ggctgagcat ccaccactcg
    59881 ctggatctcc acggtttcca gcacttctag cagtgtcgga ccgctgtacc acggcatact
    59941 ttcactttgc gaagccacgt tgtcgccttc cagtgcggag agcggcacaa agcggatatc
    60001 cagattaccc ggcagctgcc cggcaaaagt cagataatct tcacgaatac gggtgaacgt
    60061 ctcttcactg taatccacca gatccatttt gttgatcgcc acgaccagat gtttgatccc
    60121 caacagtgtg gagataaaac tatgacgacg ggtttgatcg agcacgcctt tacgggcatc
    60181 gatcagtaag atcgccagtt cacatgtcga tgcgccagtc gccatattgc gggtgtactg
    60241 ctcgtgccct ggggtgtcgg cgataataaa tttacgcttc tcggtagaga aatagcggta
    60301 ggccacatca atagtgatgc cctgttcgcg ctcagcttgc aggccgtcca ccagcagtgc
    60361 cagatccagc ttttcgccct gggtgccgtg acgcttactg tcgttatgca gcgatgagag
    60421 ctgatcttcg tagatttggc gggtatcgtg cagcaggcga ccaatcaggg tacttttgcc
    60481 gtcatcgacg ctaccacagg tcagaaaacg cagcaggctt ttatgttgtt gcgcaatcat
    60541 ccatgcttcg acgccgcctt cattggcgat ttgttgtgca agtgcggtgt tcatcttaaa
    60601 aatacccctg acgttttttc agctccatag accccgcctg gtcgcggtca attacgcggc
    60661 cctgacgttc actggtggtg gaaaccagca tctcttcgat gatctccggc agtgtttgcg
    60721 catttgactc caccgcgccg gtcagcggcc aacagcccag tgtgcggaaa cgcaccatcc
    60781 gttttttaat cacttcgccc ggttgcaggt cgatacggtt gtcatcaatc atcatcaaca
    60841 taccgtcgcg ttccagaacc ggacgttccg cagcaagata cagcggaaca atgtcgatat
    60901 tttccagcca gatgtattgc cagatatcct gctcggtcca gttagagagc gggaagacgc
    60961 ggatgctttc gcctttgtta atttgcccgt tgtagttgtg ccacagctcc gggcgctgat
    61021 ttttcggatc ccagcgatgg aagcggtcac ggaaagagta gattcgctct ttagcgcggg
    61081 atttctcttc gtcacggcgc gcaccaccga aggcggcatc aaaaccgtat ttgttcagcg
    61141 cctgtttcag gccttcagtt ttcataatat cggtatgttt cgcgctgccg tgtacgaatg
    61201 gattaatccc catcgccacg ccttccgggt ttttatgcac cagcagctcg cagccgtagg
    61261 ctttcgccgt acgatcgcgg aactcataca tctcgcggaa tttccagccg gtatcgacat
    61321 gcagcaacgg gaaaggcagc gtacctggat aaaacgcctt gcgcgccaga tgcagcatga
    61381 cgctggaatc tttaccgata gagtagagca tcaccggatt tgagaattct gcagccacct
    61441 cgcgaataat gtggatgctt tccgcctcca gttgccgcag gtgggtaagt cgtttttgat
    61501 ccataaccgt tcctttgcaa taccgctatt ttcttgccat cagatgtttc gactataggg
    61561 agcgtaagag aacgaatgaa attaccaatt agaatgagta gttccttaac ggaataacga
    61621 tttggcaaag ctaatatcaa aaagtgctta aggcaccgga tttcgggcgt ttaggaagat
    61681 ttgaaattgt tttagcgcag cggcagtttc atactatgac ggtaaaaaaa tttgcatggt
    61741 atttaaggac tcactatgtt ttccgcattg cgccaccgta ccgctgccct ggcgctcggc
    61801 gtatgcttta tcctccccgt acacgcctcg tcacctaaac ctggcgattt tgctaatacc
    61861 caggcacgac atattgctac tttctttccg ggacgcatga ccggaactcc tgcagaaatg
    61921 ttatctgccg attatattcg ccaacagttt cagcaaatgg gttatcgcag tgatattcgg
    61981 acatttaata gtcggtatat ttataccgcc cgcgataatc gcaagagctg gcataacgtg
    62041 acgggaagta cggtgattgc cgctcatgaa ggcaaagctc cgcagcagat catcattatg
    62101 gcgcatctgg atacttacgc tccgctgagc gatgctgacg ccgatgccaa tctcggcggg
    62161 ctgacgttac aaggaataga tgataacgcc gcaggtttag gtgtcatgct ggaactggca
    62221 gaacgcctga aaaatacgcc taccgagtat ggtattcgat ttgtggcgac cagtggtgaa
    62281 gaggaaggga aattaggtgc tgagaattta ctcaagcgga tgagtgacac cgaaaagaaa
    62341 aatacgctgc tggtgattaa tctcgataac ttaattgttg gcgataaatt gtatttcaat
    62401 agtggtgtaa aaacccctga agcagtaagg aaattaacgc gcgacagggc gctggcaatt
    62461 gcgcgtagtc atggaattgc cgcaacgacc aatccgggtt tgaataaaaa ttatccgaaa
    62521 ggcactgggt gttgtaatga cgcagaaata ttcgacaaag ccggtattgc tgtactttcg
    62581 gtggaagcga ctaactggaa tcttgggaat aaggatggtt atcagcaacg cgcaaaaaca
    62641 gccgcattcc ctgcgggaaa tagctggcat gacgtaagac tggataatca acaacatatt
    62701 gataaggcgc ttcctggaag aatagaacgt cgctgccgtg atgttatgcg gataatgcta
    62761 cctctggtga aggagttggc gaaggcgtct tgatgggttt gaaaatggga gttgggagtt
    62821 ctaccgcagg ggcggggaat tctaagtgat atccatcatc gcatccagtg cgcccggttt
    62881 atccccgctg acgcggggaa cacacatgaa tgtcggttca gaccgtgttt ttacccggtt
    62941 tatccccgct ggcgcgggga acacgaggct gaacagggag ggggctaccg gcacagcggt
    63001 ttatccccgc tggcgcgggg aacacttgcc aaccgccaaa gactggaaaa ccaatctcgg
    63061 tttatccccg ctggcgcggg gaacacgaac tggcgttgag tgataaattt gacggaatcg
    63121 gtttatcccc gctggcgcgg ggaactcttc agtttcacac tcggtaatgc cgttatttac
    63181 ggtttatccc cgctggcgcg gggaactcct cggggccagc ctccccca
//
LOCUS       DAZKGF010000006        63174 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZKGF010000006 DAZKGF010000000
VERSION     DAZKGF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.006"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            304..1542
                     /gene="preT"
                     /locus_tag="ACHOQV_000377"
     CDS             304..1542
                     /gene="preT"
                     /locus_tag="ACHOQV_000377"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFP9628807.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLKGACSC"
     gene            1536..2771
                     /gene="preA"
                     /locus_tag="ACHOQV_000378"
     CDS             1536..2771
                     /gene="preA"
                     /locus_tag="ACHOQV_000378"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFP9628808.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEDNLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(2842..3852)
                     /gene="mglC"
                     /locus_tag="ACHOQV_000379"
     CDS             complement(2842..3852)
                     /gene="mglC"
                     /locus_tag="ACHOQV_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFP9628809.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(3868..5388)
                     /gene="mglA"
                     /locus_tag="ACHOQV_000380"
     CDS             complement(3868..5388)
                     /gene="mglA"
                     /locus_tag="ACHOQV_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFP9628810.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(5449..6447)
                     /gene="mglB"
                     /locus_tag="ACHOQV_000381"
     CDS             complement(5449..6447)
                     /gene="mglB"
                     /locus_tag="ACHOQV_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFP9628811.1"
                     /translation="MNKKVLTLFAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(6727..7767)
                     /gene="galS"
                     /locus_tag="ACHOQV_000382"
     CDS             complement(6727..7767)
                     /gene="galS"
                     /locus_tag="ACHOQV_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFP9628812.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNLAM"
     gene            complement(7909..9066)
                     /gene="yeiB"
                     /locus_tag="ACHOQV_000383"
     CDS             complement(7909..9066)
                     /gene="yeiB"
                     /locus_tag="ACHOQV_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFP9628813.1"
                     /translation="MGRNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPARSKTSR"
     gene            complement(9083..9751)
                     /gene="folE"
                     /locus_tag="ACHOQV_000384"
     CDS             complement(9083..9751)
                     /gene="folE"
                     /locus_tag="ACHOQV_000384"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFP9628814.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            10009..10845
                     /gene="yeiG"
                     /locus_tag="ACHOQV_000385"
     CDS             10009..10845
                     /gene="yeiG"
                     /locus_tag="ACHOQV_000385"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP9628815.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(10877..12856)
                     /gene="cirA"
                     /locus_tag="ACHOQV_000386"
                     /pseudo
     CDS             complement(10877..12856)
                     /gene="cirA"
                     /locus_tag="ACHOQV_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(13149..14618)
                     /gene="lysP"
                     /locus_tag="ACHOQV_000387"
     CDS             complement(13149..14618)
                     /gene="lysP"
                     /locus_tag="ACHOQV_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFP9628816.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAVS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(14823..15704)
                     /gene="yieE"
                     /locus_tag="ACHOQV_000388"
     CDS             complement(14823..15704)
                     /gene="yieE"
                     /locus_tag="ACHOQV_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFP9628817.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            15803..16852
                     /gene="yeiH"
                     /locus_tag="ACHOQV_000389"
     CDS             15803..16852
                     /gene="yeiH"
                     /locus_tag="ACHOQV_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFP9628818.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            16926..17783
                     /gene="nfo"
                     /locus_tag="ACHOQV_000390"
     CDS             16926..17783
                     /gene="nfo"
                     /locus_tag="ACHOQV_000390"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP9628819.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTSAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            17786..18874
                     /gene="yeiI"
                     /locus_tag="ACHOQV_000391"
     CDS             17786..18874
                     /gene="yeiI"
                     /locus_tag="ACHOQV_000391"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9628820.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLDQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(18930..20180)
                     /gene="nupX"
                     /locus_tag="ACHOQV_000392"
     CDS             complement(18930..20180)
                     /gene="nupX"
                     /locus_tag="ACHOQV_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP9628821.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKSFINRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(20280..21221)
                     /gene="rihB"
                     /locus_tag="ACHOQV_000393"
     CDS             complement(20280..21221)
                     /gene="rihB"
                     /locus_tag="ACHOQV_000393"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFP9628822.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            21351..22049
                     /gene="yeiL"
                     /locus_tag="ACHOQV_000394"
     CDS             21351..22049
                     /gene="yeiL"
                     /locus_tag="ACHOQV_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFP9628823.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(22117..23367)
                     /gene="psuT"
                     /locus_tag="ACHOQV_000395"
     CDS             complement(22117..23367)
                     /gene="psuT"
                     /locus_tag="ACHOQV_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP9628824.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(23461..24399)
                     /gene="psuG"
                     /locus_tag="ACHOQV_000396"
     CDS             complement(23461..24399)
                     /gene="psuG"
                     /locus_tag="ACHOQV_000396"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFP9628825.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELAHTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(24387..25328)
                     /gene="psuK"
                     /locus_tag="ACHOQV_000397"
     CDS             complement(24387..25328)
                     /gene="psuK"
                     /locus_tag="ACHOQV_000397"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFP9628826.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHGEFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(25752..27443)
                     /gene="fruA"
                     /locus_tag="ACHOQV_000398"
     CDS             complement(25752..27443)
                     /gene="fruA"
                     /locus_tag="ACHOQV_000398"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFP9628827.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVATSG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKEGAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYISFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(27460..28398)
                     /gene="fruK"
                     /locus_tag="ACHOQV_000399"
     CDS             complement(27460..28398)
                     /gene="fruK"
                     /locus_tag="ACHOQV_000399"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9628828.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFDVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(28398..29528)
                     /gene="fruB"
                     /locus_tag="ACHOQV_000400"
     CDS             complement(28398..29528)
                     /gene="fruB"
                     /locus_tag="ACHOQV_000400"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFP9628829.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            29896..31077
                     /gene="setB"
                     /locus_tag="ACHOQV_000401"
     CDS             29896..31077
                     /gene="setB"
                     /locus_tag="ACHOQV_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFP9628830.1"
                     /translation="MHNSPAVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVTVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(31074..31328)
                     /gene="yeiW"
                     /locus_tag="ACHOQV_000402"
     CDS             complement(31074..31328)
                     /gene="yeiW"
                     /locus_tag="ACHOQV_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9628831.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            31483..32055
                     /gene="yeiP"
                     /locus_tag="ACHOQV_000403"
     CDS             31483..32055
                     /gene="yeiP"
                     /locus_tag="ACHOQV_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFP9628832.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            32278..33744
                     /gene="yeiQ"
                     /locus_tag="ACHOQV_000404"
     CDS             32278..33744
                     /gene="yeiQ"
                     /locus_tag="ACHOQV_000404"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9628833.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQSGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARALMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLARE
                     TDWSLLALGVAGWMRYVSGVDDAGNTIDVRDPLSDKIRDLVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            33862..34848
                     /gene="yeiR"
                     /locus_tag="ACHOQV_000405"
     CDS             33862..34848
                     /gene="yeiR"
                     /locus_tag="ACHOQV_000405"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFP9628834.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNKNFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHGVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVMRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            34887..35600
                     /gene="lpxT"
                     /locus_tag="ACHOQV_000406"
     CDS             34887..35600
                     /gene="lpxT"
                     /locus_tag="ACHOQV_000406"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFP9628835.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            36012..36578
                     /gene="mepS"
                     /locus_tag="ACHOQV_000407"
     CDS             36012..36578
                     /gene="mepS"
                     /locus_tag="ACHOQV_000407"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFP9628836.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            36759..38315
                     /gene="pdeN"
                     /locus_tag="ACHOQV_000408"
     CDS             36759..38315
                     /gene="pdeN"
                     /locus_tag="ACHOQV_000408"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP9628837.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFTDLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLKELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            38397..40211
                     /gene="yejA"
                     /locus_tag="ACHOQV_000409"
     CDS             38397..40211
                     /gene="yejA"
                     /locus_tag="ACHOQV_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9628838.1"
                     /translation="MIVRILLLFIALFTFGAQAQTIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNAGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRLEND
                     AKNWVTRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPSSSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            40212..41306
                     /gene="yejB"
                     /locus_tag="ACHOQV_000410"
     CDS             40212..41306
                     /gene="yejB"
                     /locus_tag="ACHOQV_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFP9628839.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNTGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            41306..42331
                     /gene="yejE"
                     /locus_tag="ACHOQV_000411"
     CDS             41306..42331
                     /gene="yejE"
                     /locus_tag="ACHOQV_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9628840.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            42333..43922
                     /gene="yejF"
                     /locus_tag="ACHOQV_000412"
     CDS             42333..43922
                     /gene="yejF"
                     /locus_tag="ACHOQV_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFP9628841.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIVFDGKPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(43926..44270)
                     /gene="yejG"
                     /locus_tag="ACHOQV_000413"
     CDS             complement(43926..44270)
                     /gene="yejG"
                     /locus_tag="ACHOQV_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFP9628842.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(44604..45794)
                     /gene="bcr"
                     /locus_tag="ACHOQV_000414"
     CDS             complement(44604..45794)
                     /gene="bcr"
                     /locus_tag="ACHOQV_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFP9628843.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(45822..46517)
                     /gene="rsuA"
                     /locus_tag="ACHOQV_000415"
     CDS             complement(45822..46517)
                     /gene="rsuA"
                     /locus_tag="ACHOQV_000415"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFP9628844.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            46667..48427
                     /gene="radD"
                     /locus_tag="ACHOQV_000416"
     CDS             46667..48427
                     /gene="radD"
                     /locus_tag="ACHOQV_000416"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9628845.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAVTRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            48552..48836
                     /gene="rplY"
                     /locus_tag="ACHOQV_000417"
     CDS             48552..48836
                     /gene="rplY"
                     /locus_tag="ACHOQV_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFP9628846.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(48975..49982)
                     /gene="yejK"
                     /locus_tag="ACHOQV_000418"
     CDS             complement(48975..49982)
                     /gene="yejK"
                     /locus_tag="ACHOQV_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFP9628847.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            50164..50391
                     /gene="yejL"
                     /locus_tag="ACHOQV_000419"
     CDS             50164..50391
                     /gene="yejL"
                     /locus_tag="ACHOQV_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFP9628848.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            50411..52171
                     /gene="yejM"
                     /locus_tag="ACHOQV_000420"
     CDS             50411..52171
                     /gene="yejM"
                     /locus_tag="ACHOQV_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFP9628849.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            52246..52322
                     /locus_tag="ACHOQV_000421"
     tRNA            52246..52322
                     /locus_tag="ACHOQV_000421"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52280..52282,aa:Pro,seq:ggg)
     gene            52541..53782
                     /locus_tag="ACHOQV_000422"
     CDS             52541..53782
                     /locus_tag="ACHOQV_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFP9628850.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALISAGTDPQGHEKKKREAEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEIDLDAQRWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRDYVFPSLKDPRQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            54279..54485
                     /locus_tag="ACHOQV_000423"
     CDS             54279..54485
                     /locus_tag="ACHOQV_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9628851.1"
                     /translation="MEAIKKVIFRQEVKKLIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEEDVIAWMKEREQEARGTAA"
     gene            54505..54669
                     /locus_tag="ACHOQV_000424"
                     /pseudo
     CDS             54505..54669
                     /locus_tag="ACHOQV_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(54636..54815)
                     /locus_tag="ACHOQV_000425"
     CDS             complement(54636..54815)
                     /locus_tag="ACHOQV_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9628852.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQHDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            54898..55620
                     /locus_tag="ACHOQV_000426"
     CDS             54898..55620
                     /locus_tag="ACHOQV_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9628853.1"
                     /translation="MNTNGLLTKPLIEVKIMAIKEHSLLFIHTQTPRKRNNPFHSSVL
                     CTHPGGRFLPDLSRRGYLSTKSSRANFSGGTKRGAGGGIFVLVSSSSSLAQASDCLCR
                     MISSSCVCVSCIGDSVLLVMILPCKAMRRSTSHHSADSFYPLGSIPRRFSSVRPNSLS
                     QLARLMPSLCASLSNCSFSSGEIRILNCGDCPSPLGLLSRLIVDKWSPIELAFLLLGD
                     HLNTRALKKAKPRKCHYHSQGF"
     gene            55646..55984
                     /locus_tag="ACHOQV_000427"
                     /pseudo
     CDS             55646..55984
                     /locus_tag="ACHOQV_000427"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            56015..56206
                     /locus_tag="ACHOQV_000428"
     CDS             56015..56206
                     /locus_tag="ACHOQV_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9628854.1"
                     /translation="MATIPTLVHSQTAFLWRFIIFGASEHQIIHVTAWTEREARNLCP
                     SGCVAVFAARIRQGETYAQ"
     gene            56196..56456
                     /locus_tag="ACHOQV_000429"
     CDS             56196..56456
                     /locus_tag="ACHOQV_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9628855.1"
                     /translation="MHNKTTPDAAAAALTTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIADCENGGGLCVMIVLIP"
     gene            56429..56650
                     /locus_tag="ACHOQV_000430"
     CDS             56429..56650
                     /locus_tag="ACHOQV_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9628856.1"
                     /translation="MRDDRFNSLKQEFSGFPDDAADALSSISEIMRVAFFFLCTDEHR
                     DTGLNILDIAANYADFVTEVILRKATDGD"
     gene            56652..56885
                     /locus_tag="ACHOQV_000431"
     CDS             56652..56885
                     /locus_tag="ACHOQV_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9628857.1"
                     /translation="MRDIYHETIYRVFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVINELQEIETLHDQYKAPHTGE"
     gene            56891..57190
                     /locus_tag="ACHOQV_000432"
     CDS             56891..57190
                     /locus_tag="ACHOQV_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9628858.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARAQANIQKLKTMINGFRGKK"
     gene            57187..58587
                     /locus_tag="ACHOQV_000433"
     CDS             57187..58587
                     /locus_tag="ACHOQV_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFP9628859.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADQRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHGEQVKNLYLVNRPIFPAVPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            58788..59039
                     /locus_tag="ACHOQV_000434"
     CDS             58788..59039
                     /locus_tag="ACHOQV_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9628860.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            59036..59446
                     /locus_tag="ACHOQV_000435"
     CDS             59036..59446
                     /locus_tag="ACHOQV_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9628861.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            59457..59729
                     /locus_tag="ACHOQV_000436"
     CDS             59457..59729
                     /locus_tag="ACHOQV_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9628862.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            59686..59814
                     /locus_tag="ACHOQV_000437"
     CDS             59686..59814
                     /locus_tag="ACHOQV_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9628863.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            59856..60080
                     /locus_tag="ACHOQV_000438"
     CDS             59856..60080
                     /locus_tag="ACHOQV_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9628864.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            60332..60538
                     /locus_tag="ACHOQV_000439"
     CDS             60332..60538
                     /locus_tag="ACHOQV_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9628865.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            60538..61593
                     /locus_tag="ACHOQV_000440"
     CDS             60538..61593
                     /locus_tag="ACHOQV_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9628866.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPENCIIEQAKGKKWSEQDKDMHDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            61606..61941
                     /locus_tag="ACHOQV_000441"
     CDS             61606..61941
                     /locus_tag="ACHOQV_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9628867.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            61954..62367
                     /locus_tag="ACHOQV_000442"
     CDS             61954..62367
                     /locus_tag="ACHOQV_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFP9628868.1"
                     /translation="MATNESIMTLPLASKFKVEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCIDVGTAKSILSMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKII"
     gene            62573..63115
                     /locus_tag="ACHOQV_000443"
     CDS             62573..63115
                     /locus_tag="ACHOQV_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9628869.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IDNRVNNAISQLTNDKGDIDDAELLRVRIRLLTAQAEAQELKNERDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQIPPAMLDRLKREVVKACNGSARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
BASE COUNT        15944 a        16145 c        15804 g        15281 t
ORIGIN      
        1 gttaattatt gagaataatt attacttcac tcaataagct gcggatataa cattctgaac
       61 cgcagctatt tgtgaatcct ttcacagttt aaatttcccc acgcgcatag ccttaatatc
      121 agcacgcaat atttatactc taaataattc gagttgcagg aaggcgacaa gtgagtgagt
      181 ccccaggagc ttacataagt aagtgactgg ggtgagcgaa cgcagacgca gcacatgcaa
      241 cttgaagtat gacgagtata tttagcgttc gccttaatta ctcatagcat taaggaagat
      301 cacatgccgc aacaaaatta tctggatgaa ctcactccgg cttttacacc gttactggcg
      361 attaaagaag cctctcgctg tttattatgc catgacgccc cctgtagtca ggcttgcccg
      421 gcacagaccg atccggggaa atttattcgc tcaatctact ttcgtaattt taaaggcgct
      481 gccgagacaa ttcgtgaaaa taatgccctc ggtgccgttt gtgccagagt gtgcccgacg
      541 gagaaattat gccaaagcgg ttgtacccgt gccggtgttg atactcccat tgatatcggt
      601 cgcttacagc gttttgttac tgattttgaa caacaaactg ggatggaaat ttatcagccc
      661 ggtactaaaa cgctcggcaa agtcgccatt attggcgcgg gtcctgccgg attgcaggcc
      721 agtgtgacac tgacaaacca gggttatgac gtcacgatct atgagaaaga agcacaaccc
      781 ggtggctggt tgcgtaacgg cattccgcaa ttccggttac cgcaatcagt actggatgca
      841 gaaatcgccc gtattgaaaa aatgggcgtg accattaaat gcaataacga aatcggtaac
      901 acactcaccc tggagcagct gaaagcggaa aaccgcgcgg tactggtcac tgtggggtta
      961 tcgagcggtt cggggctacc actgtttgag catagcgatg ttgagattgc cgtcgacttc
     1021 ttgcaacgtg cacgacaggc gcaaggcgat atcagcattc cacaaagcgc attaattatc
     1081 ggcggcggtg atgtcgcgat ggacgtagcc agcacgctga aagttctcgg ctgtcaggca
     1141 gtaacttgcg tagcgcgtga agagttagat gagtttccgg caagcgaaaa agagtttgcc
     1201 agcgcccggg agctgggcgt ttcgatcatc gatggattca cgccagtagc tgtggaaggc
     1261 aataaagtca cgtttaagca tgtacggtta ccgggcgaac tgacgatggc ggcagataaa
     1321 attattctcg ccgtcggtca gcatgccaga ctggatgcct ttgcggagtt agagccgcag
     1381 cgtaacacca tcaaaacaca aaattaccag acccgcgacc cgcaagtctt tgctgctggc
     1441 gatattgttg agggtgacaa aaccgtggtt tatgccgtga aaaccgggaa agaagccgct
     1501 gaggcgattc atcactattt aaagggagct tgctcatgtt aacgaaagat ctttcgatta
     1561 ccttttgtgg tgtgaagttt cccaacccgt tctgcctctc ttcttcgcct gtaggcaact
     1621 gctatgagat gtgtgccaaa gcctacgaca caggctgggg tggcgtggtg tttaaaacga
     1681 tcggtttttt tatcgccaac gaagtctcgc cgcgttttga tcatctggtg aaagaagata
     1741 ccggttttat cggcttcaaa aatatggagc agattgctga acatccgttg gaagataatc
     1801 tggccgcact gcgtcggctg aaagaagatt acccggacaa agtattgatc gcttcaatca
     1861 tgggcgaaaa tgagcagcaa tgggaagagc tggcgcgtct ggtgcaagaa gctggcgcgg
     1921 atatgatcga gtgtaacttc tcctgtccgc aaatgacttc tcatgcgatg ggtagcgatg
     1981 tcgggcaaag cccggagctg gtagaaaaat attgtcgggc agtaaaacgt ggttccacgc
     2041 tgccaatgct ggcgaagatg acgccgaata tcggtgatat gtgcgaagtg gcgctggcgg
     2101 cgaagcgcgg cggcgcagat ggcattgcgg cgattaacac cgttaaatcc atcaccaata
     2161 tcgatcttaa tcagaaaatc ggtatgccga tcgttaacgg taaatcgagt atttccggat
     2221 attccggtaa agcggtaaaa ccgatcgccc tgcgcttcat tcagcaaatg cgtacccatc
     2281 cagaactgcg cgatttcccg atcagcggta tcggcggcat tgaaacctgg gaagatgcgg
     2341 cggagttttt attgctcggt gcagcgacgt tgcaggtgac caccggcatt atgcagtacg
     2401 ggtatcggat agtggaagat atggcaagcg ggttgtcgca ttatctcgcc gatcaagggt
     2461 ttgattcgtt gcaggagatg gtaggtctgg cgaataacaa tattgtcccg gcggaagatt
     2521 tagaccgcag ttatattgtc tatccccata tcaatctcga taaatgtgtt ggctgtggac
     2581 gctgttatat ttcctgttac gacggcggtc accaggcgat ggaatggagc gagaaaaccc
     2641 gtacaccgca ttgtaatacc gagaaatgtg ttggttgcct gctttgtggt cacgtctgcc
     2701 cggtgggttg tattgatctc ggagaagtga agtttaagaa gggagagaaa gaacacccgg
     2761 taacgttgta aaaccagaga cgcatccggc atttggcacc gattgccgga tgcggcgcat
     2821 ccggcaatac ccacattcac atcatttctt acgcgcgtat ttcagtgaat ccagcgccac
     2881 ggcgaagata ataatcgccc ctttgatgat gtactgccag tatgggttta cgccgatgta
     2941 cgtcaggcca tagttgatga cggtaaaaat aatcaccccg gtcaccacgc caatcaccgt
     3001 ccctacacca ccgctgaacg atacaccacc caccacacat gccgcgatag catccaactc
     3061 atacataaag ccgaggttgt tggtggcaga gccgatacgt ccggcttcta acatcccgcc
     3121 gaaggcatag aacacgccag acaacgcgta gatcatcagc aggttcaggc cgacgttgac
     3181 accagatact ttcgccgctt ccgggttacc gccaatggca aaaatgttct taccgaagcg
     3241 ggttttgttc cacaacaccc agacgaacgc caccgcaatc aacgcgtaga aggtgatgta
     3301 agagagacgg aaactcccca gcgcgacaaa gccctgagca aaggtagaga agccactgtc
     3361 gaaaccagaa atcggcgacg ccccgacaaa gtcgtaatag agcgagttga tgccatagac
     3421 gatgatcatc gtgccgagcg tggtaatgaa cggcgtcacg ttgagatagg cgataatcag
     3481 accgttgatc aaaccgatca ccgcaccaat ggcacagaca atcagaataa ccagcgcaat
     3541 cggcatcgtc gccatttccg ggaacacttt gttggcgtta tccatggact gcaataacgt
     3601 cgccgctact actgccgcca gccccacctg gcgacctgca gaaaggtcgg tcccctgggt
     3661 gacaattaac cctgccacac cgagcgcgat aataatacgc accgatgact gggtgagaat
     3721 attacttaag ttcaacagac ttaaaaatgt tgggtcctgg aaaataataa ttgccagcaa
     3781 aactaaaaga acgacgtaaa taccgccctc tttcaggtaa gtaagaaaac ttttcttatt
     3841 taacgcactc atgggaagcc cctaatctta aaggtgcaaa gacgcaagac gcagaatttc
     3901 gttttgcgtt gttgtttttg tatcgacaat tccggaaacg agaccattgc tcatgaccag
     3961 aatacggtct gttatcccta acaactcagg catttcagag gagataataa taatcccttt
     4021 gcctttcttc gccagttcgg caattaactg atagatctca aacttcgctc cgacatcaat
     4081 accgcgcgtc ggttcatcga gcattaatat ttctggttgc gttagtagcc agcgaccaat
     4141 aatcaccttt tgctgattac cgccggagag cgaaccaatt tgcgtccgat gccccggtgt
     4201 ttttacccgc atcgaatcaa tcacccactg ggtatcgctt ttcatccgcg agttatccag
     4261 taaaccaact ttatttttgt agttgcgaat attggaaatt aacgagttaa aaccgatatc
     4321 cagataggca taaattcccg ttgagcggcg ctcttcagtt accagtgcaa atccgtggtt
     4381 tatggcttcg ttggcattat ggttattgat ctttttgccg tgcaacgtaa tggtgccagc
     4441 cgatttctcg cgaataccaa ataacgtctc aacaatatcg gtacgtttcg ctcccaccag
     4501 accggctata ccgagaatct cccctttatg cagatcaaac gagacatcgc gaatcgacgg
     4561 ctggcgcagc gacgtcaggt tacgcacttc aagaatcact tcgcccggct tgttttcttt
     4621 gtcagggaaa cgctggttaa gagaacgccc aaccatcatg gcgatgatct tgtccatcgt
     4681 cagtcctgcc agcggttcag tggcgatcca ctgaccgtcg cgcaatacgg taacttcatc
     4741 acataactgg aagatttctt ccattttatg tgagatataa acaataccgc agccgcgctc
     4801 ttttaattta cgaataatag tgaacagatg attgacctct ttttcggtta acgaagaagt
     4861 cggttcatcc ataatcacaa ttttcgcgtt ataggaaaac gctttggcga tttcgatcat
     4921 ctgcatttgc gaaacggata atgtgccgac gcgcgcacgc ggatcgatat caatatccag
     4981 ttcatcaaaa atcgctttgg tttcgcggta cattttgtcc tgatcgacaa acatgccttt
     5041 ggtgggatat cgccccagcc acatgttgtc catcaccgaa cgttgtaata ccaggtttaa
     5101 ctcctggtgt accatcgaaa taccattttc cagcgcttct ttggcagaat ggaaatcgat
     5161 ctctttaccc tggaataaaa tggtgccgga gtctttttga taaataccaa acaggcattt
     5221 taataatgtt gattttcctg cgccgttttc ccccattaat gcatggatag aatgtggccg
     5281 gacttttaaa ttaacgttat caagtgcctt aacacctgga aaagacttgt tgataccgct
     5341 catttccaac aagtattccc cggaagacgg agtcgttgag ctgaccatat aattttacct
     5401 tgttggccat acaataaggg cgcagtaaac gactgcgccc aatcagtctt atttcttgct
     5461 gaattcagcc aggttgtctt tatctacgcc aacataaggt acgcggacca ctttgttgtc
     5521 gattttccag ttggtgccat cagccgcacc tttgccatcg gccaggtttt tcgccagatc
     5581 aaaggtcgct ttcgcctggt tgttagcatc gttcagtacg gtgcccgcca gtgcaccgga
     5641 tttcaccagc gccagcgctt ctggcagcgc atcgacgcca aacaccggaa tgctggactt
     5701 gttgtgtgct ttcagtgctt ctaccgcgcc cattgccatc gcatcgttgt tggcgataac
     5761 cacttcgatt ttgttggcgt tcgggccaga cagccaggcg tccatcttat ctttcgcctg
     5821 agcggtatcc cacatagcgg tatctaactg taactgttca gttttgatgc ctttgtcgtt
     5881 caattcttta atcacgtaag tggtacgtgc ttctgcatcc ggatggcccg gctcaccttt
     5941 cagcagtacg aactgaatct gaccgtcttt gttcagatcc caaccctgat tcgccgccca
     6001 gtgtttagca atcaaatcgc cctgaataat gccggactct ttggagtcag tgccaacgta
     6061 gtaggctttg tcgtagctat ccagcgcctt acgagacggt tctttgttga agaaaaccac
     6121 cggcacgttt tgcccacgcg ctttctcaat caccgtaccc gcagctgccg ggtcaaccag
     6181 gttgattgcc agtgccttca cccctttcgc cagcaatacg tcgatctgat cgttctgctt
     6241 ggactggtca ttctgagaat cattcatcag cagctgaaca tctggcgcgg ctttagcatc
     6301 ttgctcaata gccttgcgca ccacagacat aaagttatcg tcgtacttat agattgttac
     6361 accaatgcga gtatcagcag cgtgtgcagc ggcaccgaat aacatgctgg ccatcacagc
     6421 aaacagggtt aacaccttct tattcatggt atctccggtt tttcttatgc agggtagtgc
     6481 ttgagataaa tgctcggcgg ggcagtagag ttaatgaagt gttactgaac gccgaagctc
     6541 actttttaaa attcgttctt ccatgcccgg taacgctcca gaaaacggct ttaattgttg
     6601 ttatgacgct attacctcgg tgaaagtgat tattcactgt tacatacggg ttacaacgtt
     6661 aaaacggtgc aatcatagct atcacattgt taagatactg tgaaatcact cacagattga
     6721 aagcggttac atcgccagat ttgttgagtt agtgatcgcc gccgcattct ggcgcgttgc
     6781 gacagaatgg cgacgcacta acgtcggcat aaaacagtgg ctggcacgcg gatcaatatt
     6841 gcctgctgcc ccctgcaagg ccagttcggt ggctaattta gccattgaag caatgggata
     6901 acgcacagtc gttaattgcg ggtcggtgta acgggcgatg ggaatatcat cgaaaccgat
     6961 gatggagaga tgtaacggaa tcgcaatgcc attatctttt aatgctgtca gtgcgccagc
     7021 ggccatattg tcgttataag caaatacagc ggtaagttgt agattgcgcc ccagcagttc
     7081 aaccatcgcc gcctcaccgc ccggcatgtc cggcgtacct gtgccaatcc agctttccgg
     7141 cggaataata tcctgctctt tcaacgcact catccagcct gctttacgca tggcgtcatc
     7201 ttcaatgccg tgactggaag aaagataacc aatacgttga tgaccgttat tcagcaacat
     7261 gcgcgtcgcc attcgggcac cgctgagatt atccaggcaa acgcaacgat gggcgtaccc
     7321 cggcacaacg cggttgatta acaccatacc aggaatgtta tccataaatt gtgccagttc
     7381 atcatcactc aatgcttttg agtgaacaat caacgcatta caccgctggc gaattaacac
     7441 ctcaatggcg tgacgctctt tttccgcttc atgatagcta ttgcctatta gcacgtattt
     7501 ctgatgctgc tgggcgacca aatccaccgc ttttaccagc gcaccaaaaa acgcatcaga
     7561 aacgtccatc accaccacgc caatggtatc gctaacctga gttgccagcg cctgggcatt
     7621 ggcgtttggc cgataatcca gctcactcac ggctttcatt actgcttcac gcgtgtcggc
     7681 actgaccagc gtgctgttat tgagcacccg ggaaaccgtt gccacagaga cgccagcctg
     7741 acgcgctaca tcacgaatgg tgatcatatt cactacctgt tatgagattg cagtaaatga
     7801 ctgcttgctg gcggctattt tgtcagcact gaatacagga cttcgtgaat cgagtcacag
     7861 caatggaaac ggttacagcc gttttgttaa cgaatgtgat ccagatcgtt atcttgatgt
     7921 tttagatctt gccggcccag ctgcacgcaa agttaactga cgccagagcc attccaccgg
     7981 cccctggcgg aagtaacgca gccagataac agagaagagg atattcgcca gccataccgg
     8041 aataacaaac gccagcagct ccaggcggtc aaaatgcata aacaaaccga ggtggtaaaa
     8101 aagcgtggta caaatcagcg tttgcaatag atagttggtc agcgccatcc gtccgacgca
     8161 ggcgatcgca agcaccagct taaagcggct caattgcggc cagaagccat aaaacagcga
     8221 cgcatagcca atcgcctgaa acggcgcact cagttcccgc ggcatctgaa gtaaaaaggc
     8281 acaccagcga tacgcccagt ccagctgcca ttgcagggcg atggcaggaa ggttaatggt
     8341 caccccaatc gccaccagca caaaaccagt acgacgatag tgacgtaagc tgaactgccc
     8401 tttcagccag ccgctgcgca tcagcgccgc accaatgagc atcatccctg ccagttgcca
     8461 gccatactgt gcgcccagtg ccagtaaact gttgccaaca ccatcggcac gattactgat
     8521 cgcttcaaca ccaccgtgaa gtttccagta cttttcatac aaaatagccg atgcatcagg
     8581 tgtccaggcg cggctggtct ggctatcgga aatcaacccc aataacagca aaacgccaag
     8641 ccccaccaga taaagcatga cgccggtatt aaacaggctt tttaccgatg gcgcatcgcg
     8701 caccagccgc cagcagatta agcccaccag cccgtaagcc agcagaatat cgccgtccca
     8761 gaacaataaa ccgtgaataa agcccagcaa gactaacagc gttaaccgcg actggatcca
     8821 gcgtctgcca cggggcagca acatttgcag gccagcacca aacagcagcg caaaaagcgt
     8881 gaggaatttc acctggccga tgagatcgag aaatgcccag gtccatgcat cctgcggcgt
     8941 aatagcgccg taccaggcgg gattaagata agccgccttt ggtagcccaa aggcgctgat
     9001 gtttaatagc aggatcccca gaatggcgac gccgcgaaca aaatcgagcg tgacgttgcg
     9061 ccccatggtt cctgcctttt aatcagttgt gatgacgcac agcgcgcaga aattcgtggc
     9121 gcgtattctg actggatttg aacaatccac caagagaggt cgttgttgtg gcactggttg
     9181 cgtcgcggat gccacgcgct ttcacgcagt aatgcaccgc gtcgatagag acagccacgt
     9241 tattggtgcc cagcagcgtt tgtagcgcaa tgagaatttg ctgggtcaga cgttcctgta
     9301 cctgtgggcg ctgggcaaag aactgcacga tgcggttaat ttttgacaga ccgatcaccg
     9361 aatctttcgg gatataagcc accgtcgctt tgccatcgat ggtaacaaaa tggtgttcac
     9421 aggtgctggt cagggtgata tcgcgcacgg tgaccatttc atcgaccttc attttgtttt
     9481 caatgagggt gattttcggg aaattggcgt aatccagacc ggagaaaatt tcatcgacat
     9541 acattttagc gatgcgatgc ggcgtttcca tcaaactgtc atcagccagg tcgagattca
     9601 gcagctgcat gatttcggtc atatgaccag caataaggct tttgcgcgtt tcgttatcca
     9661 tttcatgcac gggcgggcgc agcggtgttt ccagtcctcg cgcaactaac gcttcatgaa
     9721 ccagggccgc ttctttactg agtgatggca tttatgattt ctcctgcagg tgtgacgcct
     9781 ccgccctgcg tgggggcaaa gttattaagc tgatttacag cctgattatt gtgcgtgagg
     9841 cggcgcacat aatccagtat tcacagcgat aattattgta attgccgctg cctttcatca
     9901 gcagatgtta aaacatcgtt atgcaaatac ggaagtgaaa gttactcaca gcacattgaa
     9961 taaacggtat gatgaagaaa ttgcaaacaa cacaacaagg agccacgcat ggaaatgctc
    10021 gaagagcacc gctgttttga aggctggcag caacgctggc gacacgactc cagtacctta
    10081 aactgtccga tgacgttcag tatctttctc ccaccacctc gtgatcacac cccgccacca
    10141 gtgctgtact ggctttccgg attaacctgc aatgacgaga acttcaccac caaggcgggt
    10201 gcccagcgcg tggcggcaga actggggatt gtactggtga tgccagacac cagcccgcgc
    10261 ggcgaacagg ttgccaacga cgatggctac gatttaggcc agggcgcagg cttttatctt
    10321 aatgccacgc aaccgccgtg ggcgacgcat taccggatgt atgattatct gcgcgatgaa
    10381 ttaccggcgc tggttcagtc gcaatttaat gtcagcgacc gctgcgctat tagcggtcat
    10441 tcaatgggtg gtcacggtgc gctgattatg gcgctgaaaa acccgggcaa atacaccagc
    10501 gtttccgcct ttgcgccgat tgtgaatcca tgcagcgtac cctggggcat taaagcgttt
    10561 agcagctatt taggtgagga caaaaatgca tggctggaat gggacagttg tgcgctgatg
    10621 tatgccagta acgcgcagga tgcgatcccg acgcttatcg atcagggtga taatgatcag
    10681 tttcttgccg accagctgca acccgcagtg ctggcagaag ccgcccgcca gaaagcgtgg
    10741 ccgatgacgc tgcgtattca gccgggatat gatcacagtt actacttcat cgcctctttt
    10801 atagaggatc acctgcgctt ccatgcgcag tatttactga agtgaaagtc cgcccggttc
    10861 gccgggcatc ttctcatcag aagcgataat ccactgccat aaagtaacga cgtccgtctt
    10921 cgttatagct gtagtcgtca cgactgagat ccttgtcgcc aaggttcagc acgcctgcgc
    10981 gcagtttgac gtctttagtc acctgccagg ccgcaccggt attccagatg gtataaccgc
    11041 ccggtgtttt agccgtcgcg ctgtcggcgc gtttctgccc ggtatagtgt ccggaaacat
    11101 agaatgacca gtcttccagc gccagcggtt tccagtccag cgtaccgtta gcggtatgga
    11161 acggcagatc ggataaaggt ttattttcgc cgttgctgac atcacgacca tcgttgtagg
    11221 tgtagttgag tgacagtttc cattcatcgt tgaacggaat tttcagctcg gtttccacgc
    11281 cctgaatacg tgctttgtta acgttgtagt agctaaatac cggcacaccg ttcgctgtaa
    11341 aaccaacaaa gttttggtag cctggtgcag cattgacgtc agacgtacgg ctgatgctga
    11401 tacgatcttt cacatcgtta cggaaaacgg taacgctgga ttcaacccct tccagccagc
    11461 cttcttcgcc catgtagtaa agccccagct cccaactttc gctggtttct ggtttcagat
    11521 ccgggctacc cacaatctta catgcgccac ggcaggaatt gctcgtccag tcagggctaa
    11581 gttgcaacag agaaggtgct ttaaatgccg tcgcccagcc ccctttcacc gttacggtgt
    11641 cggtggcgtt ataaaccagg taggcacgcg gactccagtg ttcaccgtag gtttcatgat
    11701 cgtccatacg tacgccggtc gtcagcgcca gcggctcgaa gatccgccat tcatcttcaa
    11761 caaatagcgc gtactggctg gcagatgttt tggagctggt tcccccagtc aggttcaccg
    11821 catcgctaag tttgtcgtga cgccattcac cgccaaccgt gagaaactga ttaatcgccg
    11881 tcagcggcaa cgtgtatttg ccgtcgaccg tattgctttc ggaagttatc gggctgctgt
    11941 tgccagggtt tttgttctcg actttctcac cgtagtattt cagttcgctg gtgccgtaat
    12001 cccaacgccc attatggctg acggagtagt tctggcgttc caggcggttt ttgtccagcg
    12061 agtcggaatc acgatcctga cggtcgaaac cgtatccggc agtaaaatcg tgattttgat
    12121 tcggtgtcca ggcaaattcg acattgccgt cgcggctgga gaatccttca atacgcggcg
    12181 tttctccggt atcggtggtc gttgagtttt gcgggtcatc cttttcacgt tttgccaggc
    12241 tgccgtaagc tttcattccc agcacgccgt caattaatgg tccgctggta aagaattgac
    12301 cgttataggt atcaccgcga tcgcgatgtt cctgaacggt ggtatcgacg gtaacggtgc
    12361 ccgaccattt ctgaccgatt tttttggtga tgatattcac tacaccgccg agcgcatcgg
    12421 aaccgtacag cgacgacatc gggccacgga ccacttcaat acgttcgatg gaatcgaccg
    12481 ggatccagtt cagatcgaaa tcattgtggc ggaagacggc attgcgggag ttaacgcgtt
    12541 taccgtcgac gagaatcagg gtgtagctgc tgtccagacc acgaatacta acgcccttac
    12601 ggttatcccc ttcgttcgtc agttgtacgc caggcacttc tttgaggaca tccttcagat
    12661 tctgtaccgg ttttcgctgc aggtcttcct aggtaatgac gctgatactg gcaggtgcat
    12721 ctttaaggtt ttgctcaacg gaagatgcag tgacaaccat cgtttcgcca tcatcatcga
    12781 ccgctaacac aggccatgca caagaaatag cggacaaaca cagcccgacc cgtacgaaag
    12841 ggttcaacct aaacattcca tatctccatg aggtaactac gaaaataaaa tgggttatcg
    12901 ctcacatctt cttcacgtcc ccttgcgtac ggcagcatcg cggtggactt atttgtatgc
    12961 aggtgatcat cccagaaagc cagaccagcc tctgatttgt cagccttttt tgattgcggc
    13021 gtaacgataa tgcaaacgat aacaattatc aatccaaatg ttaaatttta tatccgcatg
    13081 atttgtcagg gaaatggaat tgaaaaagcc ctctcggttg agagggctta gcaaggaagg
    13141 gagaaaactt atttcttatc gttctgcggg aacttcattt cgctgtagcg tacaaagtga
    13201 gttcctttaa tcagcttgta gccgaaccaa ataatcagga acagcgggat accaatatac
    13261 gttgccgcta cgccgcccca gtcaatggta tctttcagga acgcttcata gttctggccc
    13321 aaagtgataa tcagacacag aatgaatgcg aagatcggcc ccagtgggaa gaaacctgaa
    13381 cggtacggca gatcgttaat gtcgtgtccc tgcaatacgt aaccgcgacg gaagcgatag
    13441 tggctaacgg caatccccag ccaggcgata aaaccggtca tcccggaggt gttcagcagc
    13501 cacaggtata ccgtctggtt gccaaacatg gaggtcagga agcacagacc ggcaatcacc
    13561 gtcgtcgcat acagcgcatt acgcggcacg ccaccacgcg acagtttagc gaaaatgcgc
    13621 ggcgctttac cgtcacacgc cagggtgtac agcatacgag tagacgcata cataccggag
    13681 ttacccgctg acagcaccgc cgtcagaata actgcgttca tcaccgctgc agcagagagc
    13741 agacccgcgt gctggaacac cagggtgaac ggactgacgc tgatgtcttt aacatcgtta
    13801 cgcagcaggc tcggatcggt gtacggaata atcaggctga taatcaggat cgcgaacaca
    13861 tagaacaaca ggattcgcca gaacacctga cgtaccgcgc gtggaatgtt tttcgccgga
    13921 tcttcggact cgcctgcagc aataccgatc agctcggttc cctggaaaga gaagccgaca
    13981 atcatagcta cgccgatcat cgccgcaaaa ccaccagcaa acggcgcttc gccgattgtc
    14041 cagttgctcc agcccgcagg ctgcgcgcct ttgaagatac cgataatcat cagcacgcca
    14101 acgatgataa agacaataac tgtcgtgact ttgatcagtg agaaccagta ttccgcttca
    14161 ccaaagccac gaactgagat gtagttcagc aggaagataa cgccgaggaa cagcgcactc
    14221 cagatccagc ccggtgtatc cgggaaccac cagctcatga ccagctgagc tgcaaccagg
    14281 tcaacggcga tagtcaccgc ccagttgtac cagtagttcc agcccagcgc gaagccaaag
    14341 ccttcttcaa catagttctg accgtaagtg gcaaacgaac cggaaaccgg catatatgca
    14401 gccagttcac cgagactggt catcaggaag taaaccatca ggccaatcaa catatacgag
    14461 agcaatgccc cgcccggacc tgcctgagaa atcgttgcgc cagaggcaac aaaaagacct
    14521 gtaccgatgg aaccgccaat ggcaatcatc gtcaggtgac gcgcctttaa ttcacggcgt
    14581 aagcccggcg cttctgtggt tttagtttcg gaaaccatac aaaaatgcta tccatcttaa
    14641 aaatgaggcg cgattgtagc agacgattgg caatccttcc ggcagaaata cacggttata
    14701 agacaccttc atgatcgccc agggattata agtaaagcag ccaatctctt ttctggcgaa
    14761 tgcatgctga gggcgctgcg tcacgaaatc gacacagcac cagcatgttc ttgtacagca
    14821 acttaacgcg gtacattcgc gggatcgcaa tagtccagaa agcgccgtag cgcgttggaa
    14881 aggtgttttt gccgatgatg tatacgccac aacgtacgca tcaggcgcgg cagagggacc
    14941 gcaacttcac ttaatgtgcc tgcctgcaat tgatcttcaa tcacccgtcg cgacaggcaa
    15001 ctaattccca acccatgacg caccgcatgt ttgattgcct cggagttacc taattccatc
    15061 gccatctcaa acttcggtaa atgtgacagc aacagataat cgacaatctc ccgcgtgccg
    15121 gagccgcgtt cacgcaggat ccacggcgca gcggccagct gttctaaggt gaccggacca
    15181 cgagccaatg gcgaagtcgg cgcggcgaaa accaccagct cgtcttccag ccacggttca
    15241 gaaatgattt cagtgctgtg gcacggtcct tcaataaagc caatatcaac gcggaaatcc
    15301 agcaccgctt gcatcacgtc ctggctgttc ccaacgctaa gttcaatcgg caactgcgga
    15361 taatcatggc gataacgggc aatgactgca ggcagaatgt agttaccgat agtactactg
    15421 gcatagatac gaatcgcgcc gttgtcttcg cgaaacagtt gttcgatttc aaccgcctgt
    15481 tcaagcagtg ccaacgcgcg cggatagagc agccgcccgt gttcattaac aaccagtctt
    15541 ttccccacgc gatcaaacag ttgcacgcca agctgccctt ccagatcggt caaggctgcg
    15601 ctcactgctg attgcgacaa cgccagcatc accgacgcct gggtggttga tccacttttc
    15661 aatacttctg caaaaacttc caactgccgg agggtgatgt gcatagtcgc ttaccactta
    15721 taaagattaa ttataaatat ataatcaatt ttatttttaa accagttagt cgtaacctta
    15781 tgcccggtta aaggagaggg ttatgacgaa tatcacctta cagaaacaac atcgtacact
    15841 gtggcatttt attccggggt tagccctgag tgcagttatc accggggtcg ccctgtgggg
    15901 tggttccatt cccgcggttg cgggtgccgg gtttagtgcc ctcaccctcg caatcttgtt
    15961 ggggatggtt ttaggcaaca ccatctatcc gcacatctgg aaaagctgtg acggtggtgt
    16021 gctgtttgcc aagcaatatt tattacgtct gggtatcatt ctttatggct tccgtctgac
    16081 gttctcgcaa attgccgatg tcggtatcag tgggatcatc attgacgtgc tgacgctgtc
    16141 cagtaccttc ctgctggctt gcttcctggg gcagaaagtg tttggtctgg ataagcacac
    16201 cagctggttg atcggtgccg gtagcagtat ctgtggtgct gccgcggtac tggcgactga
    16261 gccggtagtg aaagcggaag ccagtaaagt aaccgtggct gttgcaaccg ttgttatctt
    16321 cgggaccgtc gcgattttcc tctacccggc gatatatccg ctgatgtctc aatggtttag
    16381 tccggaaact ttcggtatct acattggttc tactgtgcac gaagtggcgc aggtggtggc
    16441 ggcaggtcat gccatcagcc cggatgcaga aaacgcagca gttatttcca aaatgctgcg
    16501 cgtgatgatg ctggctccgt tcctcatcct gctggcggcg cgtgttaaac agctgtctgg
    16561 ggcgaacagc ggcgagaaaa gcaaaatcac tattccgtgg tttgccatct tgttcatcgt
    16621 agttgccatc tttaactcgt tccacctgtt accgcagagc gtggtgaaca tgctggtaac
    16681 gctggatacc ttcctgctgg caatggcgat ggcggcgctg ggtctgacca ctcacgtcag
    16741 cgcgctgaaa aaagccgggg cgaaacctct gctgatggca ctggtactgt ttgcctggct
    16801 gattgttggt ggtggtgcga ttaactatgt gattcaaagc gtcatcgcat aaaccactac
    16861 atcttgctcc tgttaacccg ctatcattac cgttttcctc cagcgggttt aacaggagtc
    16921 ctcgcatgaa atacattgga gcgcacgtta gtgctgctgg cggtctggca aatgccgcaa
    16981 ttcgcgccgc cgaaatcgac gcaaccgcgt ttgccttgtt caccaaaaac caacgtcagt
    17041 ggcgtgccgc accgctcacg acgcaaacca tcgatgaatt caaagccgcc tgtgaaaaat
    17101 atcactacac atcggcgcaa attcttcccc acgacagtta tctgattaac ctcggacatc
    17161 cggtcgctga agctctggaa aaatcgcgcg atgcgtttat agatgaaatg cagcgttgcg
    17221 aacagctggg gctttctttg ctcaacttcc accctggcag ccatctgatg cagatttcag
    17281 aagaggattg ccttgcgcgt attgccgaat ccatcaacat tgcgctggat aaaactcaag
    17341 gtgtgacagc ggtgattgaa aacaccgccg gtcagggcag taacttaggg tttaaattcg
    17401 aacatctcgc ggcgattatc gacggcgtgg aagataaatc ccgcgtcggc gtctgcattg
    17461 atacctgcca tgcttttgct gccgggtatg atttgcgtac ttctgccgaa tgcgagaaaa
    17521 cgttcgcgga ttttgcccgt attgttggct ttaagtatct gcgcgggatg caccttaacg
    17581 atgcgaaaag cacctttggc agccgcgttg accgccatca tagcctcggt gaaggcaata
    17641 ttggtcatga tgcgttccgc tggatcatgc aggacgaccg tttcgacggc attccgctga
    17701 tccttgaaac catcaacccg gatatctggg cagaagagat cgcctggctg aaagcgcaac
    17761 aaactgaaaa agcggtagcc tgaagatgaa caaccgggag aaggagatcc ttgcaatttt
    17821 acggcgtaac ccgctgattc agcagaacga aattgcggat atgctgcaaa tcagccgttc
    17881 gcgcgttgcg gcgcatatta tggatttaat gcgcaaaggc cggattaaag gcaaaggtta
    17941 cattctcacc gagcaggaat actgcgtagt ggtggggaca atcaatatgg atattcgcgg
    18001 gatggcggat atccgttacc cgcaagcggc ttctcatccc ggtaccattc attgctcggc
    18061 aggcggcgtt ggacgcaata tcgcccacaa cctggcgctg ttaggccgcg acgtccattt
    18121 gctttcagtg attggtgatg acttttatgg cgaaatgctc ctggaagaaa cgcgccgcgc
    18181 cggcgtgaat gtctccggct gcgttcgttt acatggccaa agcacatcga cgtatctggc
    18241 aattgccaat cgagacgatc aaaccgtgct ggcgattaac gatacccatc tgctggatca
    18301 gttgacaccg caactactga acgggtcgcg cgatttactt cgtcatgcgg gcgtggtact
    18361 tgctgattgt aacctgacag ccgaggcgct ggaatgggtc tttaccctcg ctgatgaaat
    18421 cccggtgttt gtcgataccg tttcagaatt caaagcgggc aaaatcaaac actggctggc
    18481 gcatattcac accctgaaac ccactttacc ggagctggaa attttatggg gacaggcgat
    18541 caccagcgat gctgaccgta atgccgcagt gaatgcgttg catcagcaag gcgttcagca
    18601 actgtttgtt tatttgcccg atgagtcagt ttattgcagc gaaaaggatg gagagcaatt
    18661 tttgctgaca gcgccagcgc atacgacagt agacagtttt ggtgctgacg atggttttat
    18721 ggcgggcctg gtatatagct ttctggaggg aaacaatttc cgtgacagcg cccgttttgc
    18781 gatggcctgc gcggcaattt cacgcgccag cggcagctta aacaacccta ccctgtctgc
    18841 cgataacgcg ctttcattag tgccgatggt gtaacaatgt tgccggattc gacgcttgac
    18901 tacatcttat ccggctcata actcgcgcac tacgctaagc caataaagaa tcctgcaatg
    18961 gttgcactca tcaggttaga aagtgtcgcc gccgccagcg cgcgtaaacc aagctgggcg
    19021 atttccggtg cacggtgtgg cgcaaccgca gaaaacgccc ccaccaccac cccgatagaa
    19081 ccaaagttag cgaaaccgca caacgcgaag gaaataatcg ccacggtttt agcatccaga
    19141 gtgccagctg tttgcagata aggtgagaaa ttgaggtaag cgacaaattc atttattgcc
    19201 agtttctgtc caatcaaact cccggcaaga ttcgcatcac tccagtcaac acccattacc
    19261 cacgccagtg gggccagcag gtaacctaaa atggactcca gcgaggcatg ttcaaagcca
    19321 aaccagccgc caacgccgcc gataataccg ttaattaacg caatgatggc gacaaatgcc
    19381 atcactactg tcgccacacc tgcggcgatt ttcagcccgg tcattgcccc tgtcgcagcg
    19441 gcttcaataa tgctttttgg cggtgtttcg gtgaaagaga ggttattaaa ggaaacctgc
    19501 gaagattctg ttgccgggct taacaggcgg gcaaacaaga tcccgccggg gatcgccatt
    19561 aacgatgccg ccagcagata ttccacaggc acgcccagtg cggcgtaacc aatcattgtc
    19621 gaaccagcaa tcgaggccat cccgctacaa atcgctgtaa ataattcatt gcgattcaga
    19681 cgattgataa aggatttgac gattgccgga atttcgtttt gcccgaggaa aatggtagtg
    19741 accgcgacga atgactcgat cttgctgata ttcaatgctt tctggaagat accgccgaga
    19801 atgcgaatta agatccccat cacaccgatg tagtagagaa tactcaccag tgctgtaacg
    19861 aagataattg ccggtaatac cctgaaacca aagataaatc ctgcgccatc aaataaggta
    19921 tccatttttg gtccgaccag agaaccaaag ataaatgcgc tacctgcatc gctgtacgcc
    19981 atcactttat gcacgccaaa agcgactttt tctgcgaccc aacgccctgg tggtaaccaa
    20041 agcataatgc cgccaatcac gacctgtaac actaacgccg cgccaacggt acgcaggctg
    20101 atcttcttct tgtttactga cagcaaaaac gcaattgcca gcaataccac cattcccaga
    20161 acacttctca tgacatccat agttattatc tcttcatgtc attttgggcg acggctttct
    20221 gcccatcaga aatagccggg cctgcatctt gcgatactgt cccggcttgt tatcaacact
    20281 taatgggttt tgatgtagcc gcgcacgcac tcttcgacta atccccagaa ccagtcagta
    20341 tcaatagtga tgccgacttt ggtattggcg ggcttgccaa gaacgcccag ctcgtcgcag
    20401 accgtacgcc cgtagcaagg gccgctgtta acgtccactt cgacgtacat ctcctgggtt
    20461 ttaatgccat cagggttaat cagataaccg atgcaggtgg cgtcatgcac cgggccgcca
    20521 gccaggccgt agttttcgaa ctgggttttg agagtgaagt tcatgatgtc gctgaacagc
    20581 tctccggcag ggccgcctgc cctttccatc cgagcaatca cgtccggggt gcaaacggtc
    20641 tggttggtga gatcgaggcc catcatcact aatggaacgc cggaggtgaa caccacgcgt
    20701 gcggcttccg ggtcagcaaa gatgttgaac tcggcagatg gggtgaagtt gccagtaccg
    20761 taagcgccgc ccatcagcac aatttcacgg attttcggca ggatcgcggg ttgcatacgc
    20821 attgccaccg cgatatttga aagtgggcca accggcacca aagtgatatc gccatcgctt
    20881 gccatcaggg tatcaatgat atatttcacc gcatgagtgc tttctgcctg gcgggtcagc
    20941 ggctcgaata ccgggccatc cagtccggtt tcaccgtgaa tattatcggc aacgatttgt
    21001 tgacgcataa tgggctgtgg catccccgca taaaccggaa cattaatctc cagtttctgg
    21061 caaacattca ggccattaat taatgtttta tcaagcgtct gattacccgc cacaatggtg
    21121 atacctaata aatcaatcgc cggatgtttc gctgccatca ttatggcaat agcatcatca
    21181 tgacccggat cacaatccag aataattttt ctcttttcca ttgtttattt cctctgtttc
    21241 cagttgcgtt attttttcta cagcaaagaa aattcgaccg ggccgatgat tgaatcttaa
    21301 caacagcgta agtatgctaa atatgagaaa tctcatagcg gataaacatc gtgaaagaaa
    21361 tccacaataa tgaccttaag cagcaattga tgagtgaatc tgcgtttaag gattgctttt
    21421 caacggatgt ttcagccgat acgcggctgt ttcatttttt agcgcgtgac tacattgtgc
    21481 aggaagggca acagccgtca tggctgtttt atctgacgcg aggccgtgcc aggctttacg
    21541 ccacgcttgc taatggtcgc gtgtcgctga tcgatttctt tgccgctccc tgttttattg
    21601 gcgagattga gttaatcgac aaagaccatg aaccgcgtgc ggtgcaggct attgaagagt
    21661 gttggtgcct tgcgctccct atgaaacatt accgcccgct gttattaaac gacacgctat
    21721 ttttacgaaa actctgcgtc accttaagtc ataaaaatta tcgtaatatt gtttctttaa
    21781 ctcagaatca atcatttccg ttagtgaatc gcctggcagc ttttatatta ctctcgcagg
    21841 aaggtgatct ttatcacgaa aagcatacgc aagcggcaga gtatttaggt gtttcttatc
    21901 gacatctttt atatgttctc gcgcaattca ttcacgacgg tttattaatt aaaagcaaga
    21961 aagggtatct cattaaaaac agaaagcagt tgtcaggact ggcactggag atggacccgg
    22021 aaaataaatt ctcagggatg atgcagtaaa aattatttgc aatagagcga ttgccggatg
    22081 tgacgcttaa taagcgcgta catccggcaa tgacactcaa gctaaaccaa taaagaaccc
    22141 ggcaatggtc gcgctcatca ggttggaaag cgttgctgct gccagcgccc gtaaaccaag
    22201 ctgggcgatt tccggtgcgc gttttggcga aatagccgaa aatgccccaa cgacaacacc
    22261 gatagaacca aagttagcaa aaccacaaag cgcaaaggag ataatcgcaa tggttttcac
    22321 ttccagcgtg ccgcccgttt gcaggtatgg ggagaaactc aggtaagcga cgaattcgtt
    22381 aatcgccagt ttctgcccaa tcaggctacc cgcaagattg gcatcactcc agtccacacc
    22441 catgatccac gccagcggtg ccagcacata gccaaaaata ctttccagag aggcattggc
    22501 gaaaccaaac cagccgccaa ttccgccgat aatgccgttg atcagcgcaa taattgcgac
    22561 aaacgccatt acaaccgtcg ccacaccagc ggcgattttc agcccggtca tcgcaccgct
    22621 cgccgccgct tcgataaagc tttttggcgg cgtttcgctg aacgacagat tttcaaatgt
    22681 cacctgcgaa ggctcggtgg ctgggctaag aatacgtgca aacaaaatac caccagggat
    22741 cgccatcagc gatgccgcta acaggtagtc aattggtacg cccattccgg cataaccaat
    22801 catcatcgaa ccagcaatgg acgccatccc gctacaaatt gcggtaaaca actcgttgcg
    22861 attcatgcga tcgataaacg gtttaacgat cgccgggatc tcattttgcc cgaggaaaat
    22921 agtggtaacc gcaacaaaag attcgatttt gctgatgttg agggctttct ggaaaatgct
    22981 gccaaggatg cgaatcagca gccccatcac gccaatgtag tacagcagac tgatgagcgc
    23041 agtgacgaag ataatcgccg gaagtacgcg aaaggcgaag ataaaacccg caccgtcaaa
    23101 gaggacatcc attttcggcc cgaccagcga accaaaaatg aaggcgctac cggcatcact
    23161 gtaagacatc actttatgaa cgcctaatgc cgcctgttct actgcccatt ttcccggtgg
    23221 gaagtagagc atgatgccac caatagcgat ttgcagcagc agtgcggctc caaccgtgcg
    23281 caaactgatg ctctttttat tcactgacaa cagaaatgct attgccagta acaccaccat
    23341 ccccacaaca cttctcatta tatccataat gattttccct tcatgccagt aaacccggcg
    23401 tcagcgccag gttttggtat gcttgatgag tacgggcgac ggctttctgc ccgtcagaaa
    23461 ttaacccgcg agacgctggt attctttggc tatttcactc gccaggatgg cgttgttgaa
    23521 caccagctgg atgttggatt tcaggctgtc accgccggtc aactcagcaa cgcgcgccag
    23581 caggaatggc gtactttctt taccaataac accctgcgct tcagcttcag ctaccgcctg
    23641 atcgatcgcc gcattgatag tgtgttccgg catagcaaac tgttccggga tcgggttcgc
    23701 taccacgagg ccaccgttca gcccgctttg ccatttcacc gccattgcac gggcaatttc
    23761 gctggcgctg tcgagacgaa tgctcacgtc aaacgggctg gtgcggcaga aaaacgcagg
    23821 cagcgctttg gtctgatagc caattagcgg tacaccgaaa gtttctaaat attcggtagt
    23881 taagcctaaa tcgagaatag atttcgcccc ggcacaaacg acggtgacat tggtgtgcgc
    23941 cagttcttgc aaatcggcag aaatatcgaa ggtatgttcc gccccgcgat gcacaccacc
    24001 aattcccccg gtggcaaata ctttaattcc ggcaagcgcc gcaataatca tcgttgaagc
    24061 aacggtggtt gcaccatttt ttccggcggc aacaacaaaa ggtaaatcgc gacgactaac
    24121 tttggtcacg ttatgccctt cacgacccag caattcaatt tcttctttgc ttaaacccac
    24181 tttcatcacg ccgccaataa tggcgatagt ggcaggtaca gcgccctgtt tacgaatagt
    24241 ttcttcaact tcaattgcgg tctgggcatt ttgtgggaac ggcatcccgt gagaaataat
    24301 ggtcgattcc agcgccacaa ccggtttttt gttttttaaa gcgtcctgca cttccgggga
    24361 aatttgtaat aattcagggg aaatttttaa ttcagacatt ctgcgttctc cactaacgat
    24421 ataacgttgg caatcgataa atcggggtta ttggtgtatt cacaggagag cgccattgac
    24481 gaacatccct gtgcgaaacg aacagattcg gcaaacggca ttccgtctac ccaacacgaa
    24541 gcaagtcccg ccatcatggc atcgcccgct ccggtaacat taatgacatt ggttttgatc
    24601 ggcgcagacc aaccactttc accgctgata tcactgtaat aaacgccgtc gccgcccatg
    24661 ctcaatacca gtcggttcag gccatgttga tggaaccagg cagcaacttt tgccacatct
    24721 tcacgccctg acaaagcaat cccactcagg gtttccgctt caaggcggtt tggcttgaga
    24781 gtgtggattt gatttagacg gtcgcgcact ttgacacatt tccacgcgga aacaggatcg
    24841 acaaatacgg gtacgttggc ggcattatcc agaatccatg ccagtgcctc ttcactgata
    24901 ttacagtccg cgacaatgac ctttgccctc tgaataaatt caccgtgctg tgcgagatat
    24961 tcagctgtaa tagcgttgct aatattcatg tcatttatag caaccagcat ttcaccggta
    25021 ttatcgagta atgataaata gctcgacgta ttttctcccg gcacaatcag gcatttatcg
    25081 acataaacac cagattgatt ggtttgcgtt agcagcgatt gaccataaaa atcactgcct
    25141 acggcgctca gtagccaggc tttattcccc agcaacgcca ggttttgtgc aatattgcgc
    25201 cctactccac caggcgtaaa ctttatttta cctggatttg aatccgcata atttaatgat
    25261 tcatgtgaat atccggcgac atcaatattc gccgaaccta taattacgac ataatccttt
    25321 tcgcgcatag cacgtcctct ggtaaaaaat taccaatcct gtcaatccac aagaatatag
    25381 atactgcaaa gacggtttaa acatgtgttc atatttaaac atgtgctcat agtatgcatg
    25441 ttttacgtaa agtaaattgc cgccagagag gcaaaatggg cagatttgag caggctcaca
    25501 aaatgcgatt aaatgcctga attacaaaag tgaggaattt gtgacgggat gcacagaaca
    25561 ggaacactaa aaaagggcag aaaataatct gcccttgagg aaataacctt agtgtttgat
    25621 gaacatttca ggtttctact gaaagataaa accatttaaa ttcataaaat tacattcaca
    25681 aactggtcat tccctttgcg ctcatcaaat gttacagggc aggaaatttc tgccctgtaa
    25741 cacacctttt attacgctgc tttcgctact gcgtccactt ccggacgttt caggaaggca
    25801 taggccaaac ccgccaccag cgtaccggca ataattgcta ccaggtaacc cagtaccggc
    25861 gtaatagcgc cagggatcag cagaacaaac agaccaccgt gcggtgccat aagtttcgca
    25921 ccaatcgcca ttgagattgc gccagtcagc gccccaccaa cgatacaaca cggcagcaca
    25981 cgcatcggat cacgagcagc aaacggaatt gcaccttccg aaataaagca cagaccaaga
    26041 accagagcgg ctttgccacc ttcctgctgc gctttgtcga atttgcgacg cgccaccatt
    26101 gttgccagac ccattgccag cggtggcacc atacctgccg ccataatcgc cgccatcggg
    26161 ccataggttt gagtactcag cagacccaca ccgaatgcgt acgctgcttt atttaccgga
    26221 ccgcccatgt cagtacacat catgccaccg aggatcgccc ccagcagaac cgcattcgca
    26281 gtccccatgg tctgcagcca gtgagtcaac ccttcgagaa tgccagcaac cggtttaccg
    26341 atcaggtaga tcatcgccag accgaccacc agactggaaa ttagcggaat gatcaggatc
    26401 ggtttcagcg cctccatact ctgtggcagt ttcagttgcg tactgattaa cttcgcaatg
    26461 taaccagcca ggaagcccgc aataataccc ccaatgaagc cagaaccggt gctgaccgcc
    26521 agcatacccc caatcagacc cggcgtgaga cccggacgat cggcaatgga gaaggaaatg
    26581 taacctgcca gtaccggcac catcagcgca aaggctgaac caccaccaat ctgcatcagc
    26641 gccgctgcca acgtacccgg ctctttaaac gcttcgatac caaaagcaaa agaaagcgcg
    26701 atacacagac cacctgcaac gaccatcggc agcatgtaag agacgcccgt cagcaagtga
    26761 cggtaagcgc ctgcaccctc tttcttacct tcagtggtcg ctgtttgagc tttgcccgcc
    26821 ggttcatacg gcgttgcttc agcaaccgct ttatccagtt cctgcgcggt tttcttcagc
    26881 gccagaccgg tagaggtgcg atacatcggt ttaccagcaa atttcgccag atccacttcg
    26941 atatctgccg ccacaatcac cagatccgct gcggctactt cttcaggggt gatcgcatta
    27001 cccgcgccaa cagaaccacg ggtttcaact ttcacccacc agccacgttt tttcgcttcg
    27061 gtttcaatgg cttcagccgc cataaatgtg tgtgctacgc cagtcgggca agctgtcacc
    27121 gcaactacgc gtttcggacc gctggtggca actggtgctg tcgcagcgac cggtgcagtg
    27181 taaggtttcg catggccttt ggcttcactc aggaacagct cagggtgcgc aactgcccgg
    27241 gaaatatcgc ccagccagac atttttaccg ttcagcgcgc tgtcgttcgg gatggaatca
    27301 ccgagaacaa tcgccatttc agcgtcgttc ggattgtcga tgatttccag ttttgctttt
    27361 cgcgccgccg cgcccagcag ggtcttcgcc atataggcgc gtgcctgacc gagattagcg
    27421 tcaataatca gcagcgtttt cattatgcct ctcctgctgt cagttaaaag gttgtaagtc
    27481 gacgcgcgcc atcattgcgg ccaactgcgg acgatcggta atacccacat tgctttgact
    27541 taccgccagg gctgcaacag ctgtcgccag acgcagtgtg tgttcactgg attcacgcat
    27601 cagcaagcca taaatcaggc caccaaccat agaatcccct gcgccaacgg tgcttacgac
    27661 atcgactgac ggtggtttgg cgatccattc gccggaggca ttaacccaaa gcgcgccttc
    27721 ggcacccagt gaaataacga catgcgcgat accttgttca cgcagcgcat gcgcagcttc
    27781 aatcacatct ttcatttcag gcagtttacg gcctgcccag atttccagct cgcggcggtt
    27841 aggtttcacc agccacggtg ccgctttcaa acctgctact aacgcttcac ggctactatc
    27901 aaagataatg caaggacact gactacgcag gcgagtcatc cagtcggtga acgcttccgg
    27961 gctgacgcct gacggtaagc ttccgctgac acagaccata tcgaactgac cgagccagct
    28021 cagagaatca gtcacaaagc gttcccagtc ggcgggggta acgtcaaaac ccgagaagtt
    28081 gaagtcggtc acttcgccgt ctttttccgt cagcttaacg ttaattcggg tgcgcccctg
    28141 tacaacctgg aaacggttgg caatgcccag ctcgctgaac agttgctgaa aaccatcctg
    28201 attgtcttta ccgaggaagc caccaacggt gacatcaatt cccaggtctt ttaatacttt
    28261 ggccacgttg atgcctttac ccgccgcatg cagaccggtg gttttcacca ggttcacttc
    28321 gccgcgttca atttccgggc agaaaccaac aaggtcataa gccggattaa gggtgatagt
    28381 agcaacacgt ctgctcatta tgcgccctcc ccaagaccag cagcgatagc gtcgccgatt
    28441 gctttcagcg cctgttcagc atctgcaccc tgggcggtaa agcgtaggcg atgacctttc
    28501 ttaacgccaa gtgccacaac tttcatcaga ctacgtccgt ttgccggttt gccggtacca
    28561 tcaaggtttg tcacggtaat atcactgtta aattgtttaa tggtattgac cagcatggta
    28621 cctggacgag catgcaggcc gtgttcattg cgcaccacaa actccgcgct taacacgtcg
    28681 tcggtcggcg catcatcgct ggtcagcagc gccagcaacg ttgccgcatc cgctttcagc
    28741 aagcggtcag ctttattgtc gagtaacaaa tcagcgagac gcttaagaac cgcaatgggc
    28801 tgatcgtcat tcatcgccac actcaccagc atggctgccg tttcgccgtc cacatcaaaa
    28861 gcatttgccg cacggcttac cgcaatcgcg ctacgcagat tgccttcggc gctatcgctc
    28921 agccagatac cctgcccgag gttcagcggt tgttcattga tggctttggt gacgaaagtg
    28981 gcgtcaactg cccccgcttc tttcagacgc gcagcgttca gcgcctgaag agtcagcaga
    29041 tcgctggcga cgatatccag cgtcagcatt tcgttgtcga gcttcagctg ctcactctgc
    29101 ttttcgccca tcagcaatgc gcgaagttct tctgctgttg ttgctgactt cagttgttca
    29161 gcaacggaat catcgctcag tacgtgggtc agctggcgta gcaggcccag atgttcatcc
    29221 gagctggcag caataccgat cgccacgtac gctacctggc cgtcacccca ggtgacgcct
    29281 tccgggaact gaaatacctg aacgccggtt ttcagcacct gatcgcgggt gtcggtagtg
    29341 ccgtgtggaa tagcaatacc attgccgagg aacgttgagg tttgctgttc gcgcgccagc
    29401 atgccattga cgtagccttc agctacatta ccggcctgca ccagcgccgc agcgacctgg
    29461 cgaatcgcct cttctttgtc tccggccttt tcgcccggat ggatgtcctg tacggataac
    29521 tggaacatag ttctcctctc ttgctgaatt gaaacgattc agcttctatg agaaaaaaag
    29581 cgccaacctg gcttagggtt aaagacaaga tcgcgctgaa acgtttcaag agagcataat
    29641 acttttgttt cagcacgcaa ggaaagccgt aaagttatga gcaaaagttt gatgtgctgc
    29701 acaatttttt gtcatttttc ctaattgctg atgggaaaat ccgttatcag caatttcatt
    29761 tcagcagcgt cagcagccaa tcagcaacag tgaaatggca ttttgattat tcgtggcgct
    29821 tttgatttcg ctgtctgttt attttctgac aagcagcgta aactccgcgt cttcctcttc
    29881 cagtgatcga ccagcatgca taactccccc gcagtcacca gcgcgaaatc gtttgacctg
    29941 acctcgacgg cgtttttaat cgttgccttt ctcaccggta ttgcgggcgc tctgcaaacc
    30001 ccgacactca gtatttttct taccgatgaa gtacatgccc gtccggcgat ggtgggattc
    30061 ttctttaccg gcagcgctgt cattgggatt ctggtgagtc agtttctcgc cgggcgctct
    30121 gataagcgcg gcgatcgcaa atcgctgatt gtcttttgct gcctgttagg cgtgctggcc
    30181 tgcacccttt ttgcctggaa tcgcaactac tttgttttgc tattcgttgg cgtctttctt
    30241 agcagctttg gctcgaccgc taacccgcaa atgtttgccc ttgcccgtga acatgccgac
    30301 aaaaccggac gtgaggcggt gatgttcagc tcttttttac gcgctcaggt ttcactggca
    30361 tgggtcattg gcccaccgct ggcttatgcc ttagcgatgg gtttcagctt tacggtaatg
    30421 tatctgagcg cagcggtagc gtttattgtt tgcggcgtga cggtgtggct gtttttaccg
    30481 tcaatgcaaa aagaacttcc gctggcgacc ggcacggttg aagcgccgcg ccgtaaccgt
    30541 cgcgatacgc tgctgctgtt tgtcatttgt acattgatgt ggggctcgaa cagcctgtac
    30601 atcatcaaca tgccgctatt tattatcaac gaactgcatc ttcccgagaa actggccggt
    30661 gtgatgatgg ggaccgccgc cgggctggaa atcccgacca tgttgattgc cggatatttc
    30721 gccaaacgtc tgggtaagcg tttcttaatg cgcgttgctg ccgtgggtgg cgtctgtttt
    30781 tacgcaggaa tgctgatggc gcattcacct gtcattctgt tgggcttgca gctgctaaat
    30841 gctattttta ttggcattct gggtggcatc gggatgctct attttcagga tttgatgccc
    30901 ggtcaggcag gttcagccac cacgctctat accaatacgt cgcgcgtggg ctggatcatc
    30961 gcaggatcag tggcgggcat cgtcgccgag atctggaatt atcacgctgt gttctggttt
    31021 gcgatggtga tgattatcgc cactctgttt tgcttactgc ggattaaaga tgtttaaggc
    31081 gcggtcagca tttccagatc gataagccag gtcattgcct gctgacgcga gttaccgcac
    31141 atttcagcgc tggcttgtaa gcctgcacag actttcggcc gcagtggcga ggtgaaaatt
    31201 ttacagcgtt gctgttcatc aagctgaatg cagggcgtat tggcgggctt gccatctggc
    31261 ataccgggaa tagggctgga aatcgaaggg gcagtgcaac acgccccgca acccggacgg
    31321 cattccatac gttgttctct taaggttaac caccggattg atgagcgcgc acagtaccac
    31381 cttttttgca ccagcaaaag tgcgaatacc acttgcccga aaggcccgtc gcgagtactt
    31441 tgtcgcgata tttttgacat tttcgactac aggaattttt cgatgccaag agcgaacgaa
    31501 attaaaaaag gtatggtact gaattacaac ggcaaactgc tgctggttaa ggacattgat
    31561 attcagtcgc ccactgcccg cggtgccgct acgctgtaca aaatgcgttt ttctgatgtc
    31621 cgtaccgggc tgaaagtaga agagcgtttc aaaggagatg atatcgttga caccgtgacg
    31681 ctgacccgcc gttacgttga cttctcctat gtcgatggca acgaatatgt ctttatggat
    31741 aaagaagact ataccccgta taccttcacc aaagatcaga ttgaagaaga gttgctgttt
    31801 atgccagagg gcggcatgcc ggacatgcag gtgctgacct gggacggtca actgctggcg
    31861 cttgagttgc cgcagaccgt tgatctggaa atcgttgaaa ccgcaccagg catcaaaggg
    31921 gcatctgcca gcgcccgtaa caaaccggcg acattgagca ctggtctggt gattcaggta
    31981 ccagaatact taagcccggg cgaaaaaatt cgtatccata tcgaagaacg ccgttatatg
    32041 gggcgtgctg actaacttca gccgcatgca gaaaagggat agctcaggct gtcccttttt
    32101 taatttatta taccaatctt ctattttgcg cttcctgaac accttacgcc cgcaattttc
    32161 actcgttgat tgatcgccct cacacttcat cgcattaaca atccagacca atttcaattg
    32221 ctgtcatata actttacact gtcattgtta attaatcgtt actaagacgt gactcctatg
    32281 aatacaatcg cctccgttac gctcccgcat catgtacacg ctccacgcta cgatcgccag
    32341 cagttgcaat cacgtatcgt tcattttggc tttggagcct ttcaccgcgc acatcaggcg
    32401 ttactgaccg atcgtgtgct gaatgcccag agcggcgact gggggatctg tgaaatcagc
    32461 ttgttcagcg gtgatcaact gatgagccag ctccgcgcac agaaccattt atacaccgtg
    32521 ctggagaaag gggcggacgg caatcaggcg ataattgtcg gtgccgttca cgaatgcctg
    32581 aatgcaaaac tggattcctt agcggcaatt attgagaaat tttgcgagcc acaggtggca
    32641 attgtttccc tgacgattac cgaaaaaggc tattgtattg acccggccac cggtgcactc
    32701 gacaccagca atccgcggat tattcacgat ctacaaaccc ctgaagaacc tcactccgca
    32761 ccgggtattc tcgtcgaagc actgaaacgc cgccgtgagc gcggccttac accgtttacc
    32821 gtgctctcct gcgacaatat tcccgacaat ggccatgtgg tgaaaaacgc ggtgctggga
    32881 atggcagaaa aacgttcgcc agaactcgcc gggtggataa aagagcacgt cagttttccg
    32941 ggaactatgg tcgaccgcat tgttccggca gcaaccgacg aatcactggc ggaaatcagc
    33001 cagcatctgg gggtaaatga tccctgcgcg attagctgcg aaccgtttat ccagtgggtg
    33061 gtggaagata acttcgtcgc tgggcgtcct gcctgggaag tcgcaggtgt acaaatggtg
    33121 aatgatgtcc tgccatggga agagatgaaa ctgcgtatgc ttaatggcag ccactctttt
    33181 ctcgcttatc tgggttacct ctcaggattc gcccatatca gtgattgcat gcaggatcgc
    33241 gcatttcgcc atgccgccag agcattaatg ctggatgagc aagcgccgac actgcgaatt
    33301 aaagatgtcg atttaacaca atatgcggat aagttaattg cacgttttgc taacccggcg
    33361 ctgaaacata agacctggca aatcgcaatg gatggtagcc agaaattacc gcaacgtatg
    33421 ctggcaggta ttcgcataca tctggcgcgc gaaacggact ggtcgttgct ggcattaggc
    33481 gttgcaggct ggatgcgtta cgtcagcggc gttgatgatg ccggaaatac cattgatgtt
    33541 cgcgatccgc ttagcgataa aattcgcgat cttgttgcgg gcagcagcag tgagcaacgc
    33601 gtcaccgccc tgctttccct gcgtgaagtt ttcggtgatg atctgccaga taacccgcat
    33661 tttgtgcagg ccatcgaaca agcctggcaa caaatcgcac aattcggcgc gcatcaggcg
    33721 ctattaaaca ccctcaaaat ttaacgattt ctgcggttaa agcggatgaa gctcgccttc
    33781 gtccgctctc cccttctctt ttctgccgtt tttagccagg attaacgctc agttaacttt
    33841 taagagtatt gcggagccgt tgtgaccagg accaacctca tcaccggttt tctcggcagc
    33901 gggaaaacca cgtcgattct tcatctgtta gcccataaag atcccaacga aaaatgggcg
    33961 gtactggtta atgaatttgg ggaagtcgga attgatggtg ctttgctcgc cgatagcggc
    34021 gcattgctga aagagatccc cggcggctgc atgtgctgcg ttaatggttt acccatgcag
    34081 gtaggattga ataccttact gcgtcaggga aaaccagacc gcttgttgat agagccgacc
    34141 ggactgggtc atccgaaaca gatcctagat ctcttaaccg cgcccgtgta tgaaccgtgg
    34201 atagatctgc gcgccacgtt gtgcattctc gatccacgcc tgctgctgga cgaaaaaagc
    34261 gccagcaata aaaacttccg tgaccagctg gctgccgcag acattattgt cgccaataaa
    34321 tccgaccgtg cgacgcccga aagtgagcaa gcgctacagc gctggtggca gcaaaatggt
    34381 ggcgatcggc aattaattca cagcgagcat gggaaaattg acggtcatct tctggatttg
    34441 ccgcgtcgca atttagccga gttgcccgcc agcgccgcgc attctcatca gcatggcgtg
    34501 aaaaaagggt tagcagcgtt aagcctgcca gagcatcaac gctggcgtcg cagtctgaac
    34561 agcgggcaag gatatcaggc ctgtggctgg atattcgacg ctgatacggt attcgacacc
    34621 attggcattc tggaatgggc gcgacttgca ccggtggaac gcgtcaaagg cgtgatgcgt
    34681 attcccgaag ggctggtgcg aatcaaccgt cagggcgatg acctgcacat tgaaacgcaa
    34741 aacgttgcgc caccggacag tcgtattgag ctgatttcca gcagcgaagc tgactggaat
    34801 gccttgcaga gcgcgctgtt gaagcttcgt ttagcgacca ccgcgtaagg ttgcctgcgt
    34861 ttttcagtaa gataattaga gaaaatatga ttaaaaattt gccgcaaata gtgttgttga
    34921 atattgtcgg cctcgcgctg tttctttcct ggtatattcc cgttaatcat ggattctggt
    34981 tgccgattga tgcggatatt ttttattttt ttaatcagaa actggtcgaa agtaaggcct
    35041 ttttgtggct ggttgcattg accaacaatc gcgctttcga cggttgttca ctgctggcga
    35101 tgggaatgtt gatgctgagt ttctggctga aagaaaacgc cccaggcaga cgacgtatcg
    35161 tgattattgg tctggtcatg ctattaactg cagtggtatt aaaccagctg ggtcaggcat
    35221 taattcctgt aaaacgggcc agcccaacat tgacttttac cgatattaac cgcgtcagcg
    35281 aactgctctc tgttcccacg aaagatgcct cacgagatag ctttcccggc gatcacggta
    35341 tgatgctgct tattttttcg gcattcatgt ggcgttattt cggcaaagtt gcaggcctta
    35401 tcgcccttat tatttttgtg gtttttgcgt ttcccagagt aatgattggc gcacactggt
    35461 ttactgacat cattgtcggt tcgatgaccg tgatattgat cggtttgccc tgggtgttgc
    35521 tgacgccatt aagtgatcga ttaatcacct tttttgacaa atcactaccg ggaaaaaaca
    35581 aacatttcca aaacaaataa ctcacagtaa ttaacatcat cagggttatt tttatagtga
    35641 ggataatcct gatgatgcgc accgtgcttt catccatcgc gcgcaaaaat cattctctaa
    35701 gtaaatgaat ggattgcatg cgtttcactc aatcgtactt taattgacca atcccgttta
    35761 ttaactttct gtatcacttt ttcttataaa aaatcatgta aaaccgctcg ccaagaccgc
    35821 accaatcggg taatctcgaa ctcgttttgc ctcggcggta gattatcctc acagcatata
    35881 attttgtgcg ttagtccaca gatttggcct taaggaattg tttcaacatg cccaggtaat
    35941 tagtctcgtg tcgcttggca tttttttata acgatatttg tcgttaagga cttcaaggga
    36001 aaacaaacaa catggtcaaa tctcaaccga ttttgagata tatcttgcgc gggatccccg
    36061 cgattgcagt agcggttctg ctttctgcat gtagtgcaaa taacaccgca aagaatatgc
    36121 atcctgagac acgtgcagtg ggtagtgaaa catcatcact gcaagcttct caggatgaat
    36181 ttgaaaacct ggttcgtaat gtcgacgtaa aatcgcgaat tatggatcag tatgctgact
    36241 ggaaaggtgt acgctatcgt ctgggtggca gcactaaaaa aggcatcgat tgttctggtt
    36301 tcgtacagcg tacattccgt gagcaatttg gcttagaact tccgcgttcg acttacgaac
    36361 agcaggaaat gggtaaatct gtttcccgta gtaatttgcg tacgggtgat ttagttctgt
    36421 tccgtgccgg ttcaacagga cgccatgtcg gtatttatat cggcaacaat cagtttgtcc
    36481 atgcttccac cagcagtggt gttattattt ccagcatgaa tgaaccgtac tggaagaagc
    36541 gttacaacga agcacgccgg gttctcagcc gcagctaata aaccgtttgg atgcaatccc
    36601 ttggctatcc tgacgagtta actgaaagca ctgcttaggc agtgcttttt tgttttcatt
    36661 catcagagaa aatgatgttt ccgcgtcttg atccaggcta tagtccggtc attgttatct
    36721 tttaaatgtt gtcgtaattt caggaaatta acggaatcat gttcatacgc gctcccaatt
    36781 ctggacgtaa gctcctgctt acctgcattg ttgcaggcgt gatgattgcg atactggtga
    36841 gctgccttca gtttttagtg gcctggcata agcacgaagt caaatacgac acactgatta
    36901 ccgacgtaca aaagtatctc gatacctatt ttaccgacct gaaatccact actgaccggc
    36961 tccagccgct gaccttagat acctgccagc aggctaaccc cgaactgact gcccgcgcgg
    37021 cgtttagcat gaatgtccgt acgtttgtgc tggtgaaaga taaaaaaaca ttctgttcat
    37081 ctgcgactgg tgagatggac attccactaa aagaattgat tccggcgctc gacattaata
    37141 aaaatgtcga tatggcgatc ttacccggta cgccgatggt gccgaacaaa cccgcaatcg
    37201 tcatctggta tcgcaacccg ttgttgaaaa atagtggcgt gtttgccgcg ctgaatctca
    37261 atctgacgcc ttctctcttt tatagttcac ggcaggaaga ttacgatggc gtcgccctca
    37321 ttattggtaa tactgcgcta tctacctttt cttcacgttt gatgaacgtt aatgaattaa
    37381 ccgacatgcc ggtccgtgaa actaaaattg cgggcattcc tctgaccgtt cggctttatg
    37441 cagatgactg gacatggaac gatgtgtggt acgcattttt actgggtggc atgagtggaa
    37501 ctttcgttgg acttctctgc tattacctga tgagtgtgcg tatgcgccca ggcagagaaa
    37561 tcatgaccgc catcaagcgc gaacaatttt acgtggtgta tcaaccggtg gtggatacac
    37621 aagctttgcg ggtaacgggc ctggaagtac tgctacgctg gcggcatcca gtagcaggag
    37681 aaatcccccc ggatgccttc attaactttg ccgaagcgca aaagatgatt gtgccactga
    37741 ctcagcacct gtttgagttg attgcccgcg atgccgcaga attagaaaaa gtactgccgg
    37801 taggcgtcaa atttggcatt aacattgcgc cggcccactt gcacagcgaa agctttaaag
    37861 cggatatcca gaaactgctc acttccctgc ccgcacacca tttccagatt gtgctggaaa
    37921 ttaccgagcg cgatatgctg aaagagcgag aagccacaca actcttcgcc tggctgcatt
    37981 cggtcggcgt agaaattgct attgatgact tcggcaccgg gcacagcgcg cttatctatc
    38041 ttgagcgttt tacgctcgat tatctgaaaa ttgatcgtgg atttatcaac gccatcggta
    38101 cggaaacgat cacttcaccc gtacttgacg cggtgctgac gctggcgaaa cgcctcaata
    38161 tgctgacggt tgctgaaggg gtcgaaacgc cagaacaggc acgatggcta agcgaacgcg
    38221 gcgttaattt catgcaaggc tactggatta gtcgcccgtt accgctggac gattttgttc
    38281 gctggctgaa gaaaccgtat acgccgcagt ggtaaggtgt gcttacgtcc cttattattc
    38341 atagtgaaag catgccggat tgcggctaat gatgagtaaa aggaaatccg ttgcagatga
    38401 ttgtgcgcat actgctgctg tttatcgctc tgttcacctt tggtgcgcag gcgcagacta
    38461 tcaaggaaag ctatgccttt gccgtactgg gcgaaccccg gtacgcattt aatttcaacc
    38521 attttgatta tgtgaacccc gccgcgccaa aaggtgggca aataacgttg tctgcgctcg
    38581 gcaccttcga taatttcaac cgctatgcac tgcgcggcaa tgcgggcgca cgcaccgagc
    38641 agctgtacga cacgctattt acgacttccg atgacgaacc aggcagttat tacccgctga
    38701 ttgctgaaag cgcacgctat gctgacgatt attcctgggt ggaggtcgct attaatccac
    38761 gcgcccgttt tcatgatggt tcgcccatta ctgcccgcga tgtagagttt acttttcaaa
    38821 aatttatgac cgaaggcgtg ccgcaatttc gtctggtcta caaaggcacc accgtcaaag
    38881 ccattgcgcc gttaaccgtg cgcattgagt tagcgaaacc cggcaaagaa gatatgctga
    38941 gcctgttttc gctgccggtc tttccagaaa agtactggaa ggatcacaaa cttagcgacc
    39001 cgctcgccac gcctccgctt gccagtggtc cgtaccgaat tacgtcctgg aaaatggggc
    39061 aaaatattgt ctattcccgc gtgaaagatt actgggcagc aaacttaccg gtaaaccgtg
    39121 gacgctggaa tttcgacacc attcgctacg attattacct cgatgataat gtcgcctttg
    39181 aagcgtttaa agccggtgcc tttgatttgc gtttggaaaa cgacgccaaa aactgggtca
    39241 cgcgctatac cggtaaaaat ttcgataaga aatacatcat caaagatgag caaaagaacg
    39301 aatcagccca ggatacgcgc tggctggcgt ttaatatcca acgtccggta ttcagcgatc
    39361 gccgggtgcg ggaagcaatc actctcgcct ttgactttga atggatgaac aaggcgttgt
    39421 tttataatgc ctggagtcgc acgaacagtt attttcagaa taccgaatat gcggccagaa
    39481 attaccccga cgccgcggag ctggtgcttc tggcaccaat gaaaaaagat ctaccgccag
    39541 aagtcttcac gcaaatctac cagccgccgg tatccaaagg cgatggctac gatcgtgaca
    39601 acctgttaaa agccgacaaa cttctcaacg aagcgggctg ggtgctgaag ggtcagcaac
    39661 gcgtaaatgc cacaacgggt cagccgctca gctttgagtt attgctacct tcgagcagca
    39721 atagtcagtg ggtattgccg ttccagcaca gcctgcaacg gctgggtatc aacatggaca
    39781 ttcgcaaagt ggataactcg caaatcacca accgcatgcg cagtcgcgac tatgacatga
    39841 tgccgcgcgt atggcgggcg atgccgtggc ccagttccga tttacagatt tcctggtcat
    39901 cggaatatat caattccact tataatgccc ccggcgtgca aagcccggtt atcgactcgc
    39961 tgatcaacca aattattgcc gcgcagggaa ataaagaaaa attactgccg ttggggcgag
    40021 cactggatcg cgtattaacg tggaattatt acatgctgcc aatgtggtac atggcggaag
    40081 accgtctcgc ctggtgggat aaattctccc aacccgctgt acgccctgtt tacagcctgg
    40141 gtattgatac ctggtggtat gacgttaata aagcgacgaa actgccgtca gccagacaac
    40201 agggagagta gatgggcgct tatctgattc gccgtctgtt gctggtgatc ccaacgttat
    40261 gggcgattat cactatcaac tttttcatcg tgcaaattgc gcctggcggt ccggtcgacc
    40321 aggccatcgc cgccattgag tttggtaata ctggtgtatt acccggtgca ggcggtgaag
    40381 gtgttcgcgc cagccatgca caaacgggcg tcggtaatat cagcgacagt aattaccgtg
    40441 gcggacgcgg attggatcca gaagtgatcg ccgagatcac tcatcgctac ggtttcgata
    40501 agccgatcca cgaacgttac ttcaaaatgc tctgggacta catccgtttt gattttggcg
    40561 acagcctttt tcgcagcgcc tcggtgctga cgctgattaa agacagtctg ccggtctcca
    40621 tcaccctcgg attgtggagc acgctgatta tctatctggt gtcgattccg ttaggcattc
    40681 gcaaagctgt ttataatggg agccgttttg acgtctggag tagtgcattt atcatcatcg
    40741 gctacgccat tccggccttt ttgtttgcca tcctgctgat tgtcttcttc gcgggcggca
    40801 gctatttcga cctgttccct ctgcgcggcc tggtttccgc taactttgat tcgctgccgt
    40861 ggtatcagaa aatcaccgat tatctgtggc atatcacgct gccggtgctg gcgacagtga
    40921 ttggcggctt tgcggctctg accatgctga caaaaaactc attccttgat gaagtgcgta
    40981 agcaatacgt ggtgacggca cgtgcgaaag gggtaagtga aaaaaatatt ctctggaaac
    41041 atgtgttccg caacgccatg ctgctggtga ttgccggttt tccggcgacg tttatcagca
    41101 tgttttttac cggctcgctg ctgattgagg tgatgttttc actcaatggt cttggcttac
    41161 tgggctacga agcgaccgtc tcgcgcgatt atcctgtaat gtttggtacc ttgtatattt
    41221 tcaccctgat tggcctgctg ctgaatattg tcagtgatat cagctatacg ctggtcgatc
    41281 cgcgtataga ttttgaggga cgttaatgtc gcgactcagc cccgtcaatc aggcccgttg
    41341 ggcgcgtttt cgtcataacc gtcgcggcta ctggtcgtta tggattttcc tcgtcttgtt
    41401 tggtttgagt ttgtgttctg aacttatcgc caacgataaa ccgttgctgg tgcgttatga
    41461 cggcagttgg tatttcccgt tgttgaaaaa ctacagcgaa agcgattttg gcggcccgct
    41521 ggcaagtcag gctgattatc aggacccgtg gctgaaacaa cggctggaaa ataacggctg
    41581 ggtactgtgg gcaccgattc gctttggtgc taccagtatc aactttgcta ccgataagcc
    41641 cttcccttct ccaccctccc ggcaaaactg gctgggaacg gatgccaatg gcggcgatgt
    41701 gctggcgcgc attctctatg gcacgcggat ctcggttctg tttggcctga tgctgactct
    41761 ctgttccagc gtgatgggcg tgctggcggg ggcgctacaa ggctattacg gcggtaaagt
    41821 cgatctctgg gggcaacgct ttattgaagt atggtcaggg atgccgacgc tgtttttgat
    41881 tattttactt tccagcgtcg tacagcctaa cttctggtgg ctgctggcaa ttaccgtctt
    41941 gtttggctgg atgagtctgg tcggcgtggt gcgggcggag tttttacgta cccgtaattt
    42001 cgactacatc cgcgcggcgc aggcgcttgg cgtcagcgat cgcagtatca tcctgcgtca
    42061 tatgttgcca aatgccatgg tcgctaccct caccttttta ccgtttattt tatgtagttc
    42121 gataaccacc ctgacctcac tcgatttcct cggcttcggt ctgccgctcg gttcaccgtc
    42181 actcggcgaa ctgctgttac aagggaaaaa taaccttcag gccccgtggc ttgggatcac
    42241 cgccttcttg tcggtggcga tattgttgtc tttgctgatc tttattggtg aagccgtccg
    42301 cgacgcattt gatcctaata aggcggtgta gcatgacgca aactctgtta gcgattgaaa
    42361 atttgtcggt gggttttcgc catcagcaaa ccgtacgcac agtagtcaat gatgtttcac
    42421 ttcagattga ggctggcgaa acgctggcgc tggtgggtga gtcaggttca ggcaaaagcg
    42481 ttaccgcgct gtcaatttta cgcctgctcc cttccccgcc ggttgaatat ctctccggcg
    42541 atattcgttt tcatggcgaa tcgctgcttc atgccagcga tcaaacgttg cgcggtgtac
    42601 gcggtaataa gatcgccatg atttttcagg agccgatggt gtcgttaaat ccattacata
    42661 ccctggaaaa acagctttat gaagtacttt cactccaccg cgggatgcgt cgggaagcgg
    42721 ctcgtggcga aattcttaac tgccttgatc gcgtcggtat ccgccaggcg gcaaaacggc
    42781 tgacagatta tccgcatcag ctctccggcg gcgaacgcca gcgggtgatg attgcgatgg
    42841 cgctgttaac gcgaccggaa ttattaattg ccgatgaacc aaccaccgcg ctggacgtct
    42901 ctgtccaggc gcagatttta cagctgttgc gcgaactgca aggcgagctg aatatgggta
    42961 tgctgtttat tactcataac ctcagcattg ttagaaaact ggcccaccgc gtggcggtaa
    43021 tgcaaaacgg tcgctgtgtc gagcaaaata acgccgctac gctatttgcc tcacccactc
    43081 atccttacac acaaaagcta ctcaacagtg aaccgtcagg cgacccggtc ccattgccag
    43141 aacctgcctc aacgttgctg gatgttgaac agcttcaggt tgccttcccc attcgcaaag
    43201 ggattttgaa gcgcattgtg gatcataatg tggtggtgaa aaacatcagt tttacgctac
    43261 gagcgggtga aacactgggt ttagtgggcg agtctggttc cgggaaaagc acgacgggac
    43321 tggcgctgct gcgactgatt aactctcagg gcagcatcgt ctttgacggt aagccactgc
    43381 aaaatttaaa tcgccgccag ctgttaccta ttcgtcatcg cattcaggtg gtatttcagg
    43441 atccaaactc ctcgctcaac ccacgactca acgttttgca gattattgag gaaggcttac
    43501 gggttcacca gccgacgctt tctgccgcgc aacgcgaaca acaagtgata gccgtgatgc
    43561 atgaagtggg attagatcct gaaacacgcc accgttatcc ggcggagttc tctggtggtc
    43621 agcggcaacg tattgcgatt gccagggcgt taattcttaa gccctcgctg atcattcttg
    43681 atgaaccaac atcatcactc gacaaaacgg tgcaggcgca aatattgacg ctattgaaat
    43741 cattgcaaca aaagcatcaa ctggcctatt tgtttatcag ccacgatttg cacgttgtcc
    43801 gcgcgttatg tcatcaggtt atcgtactgc gacaagggga agtagtggaa caaggaccgt
    43861 gcgcgcgcgt gtttgccgca ccgcagcagg agtatacgcg tcagctactg gcgttgagct
    43921 gacgcttaaa aaggattgta gtttgaaaag ggttcggcaa ttgccacacc aaaatttttc
    43981 aatcggcatg ttgcagcaaa ttcgtcctgg cgatttacaa acaggcacgg ctcaccttca
    44041 cactccagca ccgaacatgg aacttcgatg tcgcttaacg cctggcttag tttgtacatc
    44101 accgaccatg cattatcacc atccgggcta agcaatttaa gcgccaccag gctgttgtca
    44161 ccgcacggtc cattttgcgg aatcggttca accttcgaac ctgcgaaacc agcagaccag
    44221 cgatagttct gcggcagtcg atggacgatt gagagttgta atgaagtcac ttattttccc
    44281 cggaagtaca ttttacttca caatttgttt acatcaattt taacacatca tcaacaatcc
    44341 gtctttatac agatcgtaaa gattgatgcc tcgcctgcgt ggcctctatg gctctgattt
    44401 aaataatatc tgcgggcgtg gtcacgtttg cggggctata tgtacccgtt tctgcgatct
    44461 aactcaacct ttttaactac aatgatgtga ctttttacat aaattgattt tacataaaat
    44521 aaacatatat cgggggaatg atagatttgt ggttgacatg catcaacaaa ggaagccttt
    44581 tagcttcctc gttgtgcaat agatcaccgt tttttcggcc gactggcgta caaacagaag
    44641 agaatggagc tggttgcgca gaatgcaatt gaccaaatca tcggccatgc agagttaaag
    44701 gtcgcaagag aaagtaatgc gccaacaatt gcccctatgc cgaaacggaa ggttcctgcc
    44761 agcgaagatg ccgttcccgc catatgggga aactcatcaa gaatgaccgc catcgcattg
    44821 gatgacacca tcgacacgca gcccacaaac gccgcaacgc caaccaccag cgaccagaat
    44881 cccagcccca gcagtgcact gatgaccatc cacgctgcca taataaactg tatccacaac
    44941 cccgagcgga acatatttaa cgcgccaata cggcggacga agcggctgtt aaagatggtc
    45001 atcacgaaca gaaaaagaat gtttagcgca aagtaataac caaagttttc cggcgcgacg
    45061 tggttgattt caatgtagac aaacggtccg gcgcttaaga atgagaacat cccggcaaag
    45121 ctgaaaccgc tggccagcat ataactcagg acacgcttat gacggaacag cgccgcaaag
    45181 ttaccaatag tggtacgaat gtgaaaaggc tggcgacgct ccggtggtaa ggtttctttg
    45241 atcagaaaga agatcattgc cgaagccaga atcgccgcca cggcgaggat ccagaagatg
    45301 taatgccagc tcagccacac cagcacccag ccgccaacta tcggtgccat cagcggtgca
    45361 atggtcgtca ccagcatgac aaacgacatc atccgtgaga actcttcttt cgggtagata
    45421 tcacgcatca aagcgttgat aaccacgctg gccgcagccg cagccagccc gtggaagaaa
    45481 cgcatcacaa tcagctgatc gatggtttgt gccaacgcac acgccaccgc ggcggcggca
    45541 aacaccagcg taccgccaag aaccaccggc ttacgcccga agctgtctgc catcggcccg
    45601 tagattaact gccccaacgc aaagcccaga atataagtac tgagggtcat ctgcgtactg
    45661 cccgccggta caccaaactg agctgaaatt accggtagcg caggcagata catatcaatc
    45721 gacagcggca tcaacatggc cagcaggcca aggataaaaa caatagcaaa cgacgaatgc
    45781 tgtcgggtgg tcacaacggg ctcctgaaag tcattgaaaa gttagacgac gctggcaatt
    45841 tcttcttcag ttaacggacg atattcaccg ggagctaaat cagcatccag cgtaatgccg
    45901 ccaatacgtt cacgatgcag ctcaaccaca tggttaccca cggcggcgaa catgcgtttc
    45961 acctgatgat aacgcccttc gctgatggtc agacgaacct gcgttggggt aatcacttcc
    46021 agcaccgccg gcttagtgag atctttttcg ttatgcagct gcacgccttt agcaaattgc
    46081 tctgccgtat cgtcagctac gggtgattcc agcgtcacca gataggtctt ctcgcaatga
    46141 tggcgcgggg aagtaatgcg gtgcgaccac tgaccatcat ctgtcattag taccagaccg
    46201 gtggtatcga tatccagacg ccctgcagcg tgcagtttcc acgctaccgg ttcatcaaga
    46261 aaatagagca ccgttgggtg atccgggtcg tccgtggagc aaacatagcc ctgaggctta
    46321 ttgagcatga agtaacgtgg accgtgttgc tgcgccagcg ggttgccatc gtaagcgaca
    46381 tcatgttcag ggagcagttt gaacgctgca ttacggacga tttcgccatc gacggtgaca
    46441 cgattgccgc ggatttcacg cccggcaata gcacggctaa cgccgagttg ctgtgcgata
    46501 aatttatcaa gtcgcatgtg tgtgattttg cctgtaaaaa acggaggtcg ggtaatgacc
    46561 cgaaaatctg aactgttgtc tgcccagtat agcggtctaa caacatccct caagggaaaa
    46621 agatccatgg catactatta gcagaataat ctacctacgc gagaccatga tttttacact
    46681 tcgcccatat cagcaagaag ccgtggatgc cacgctcaac cattttcgtc gtcataaaac
    46741 ccctgccgtt atcgtgctgc ccaccggtgc aggtaaaagc ctggtgatag cggaactggc
    46801 gcggctggcg cgtggtcgcg tgctggtgct ggcacacgtt aaagaactgg tggcgcaaaa
    46861 ccatgcaaaa tatcaggcgc tggggctgga agccgatatt tttgccgccg ggctaaagcg
    46921 caaagagagc cacggtaaag tggtatttgg cagcgtgcag tcggtcgccc gtaatcttga
    46981 tgcctttcag ggtgaatttt cgctgttgat tgtcgatgaa tgtcaccgta ttggtgacga
    47041 tgaagagagc cagtatcagc aaatcctcac tcacctgacc aaagtgaatc cccacttacg
    47101 cctgctgggg ctgactgcca cgccttttcg actgggcaaa ggctggattt atcagttcca
    47161 ttatcacggc atggtacgcg gcgatgagaa agcccttttc cgtgactgca tttatgagct
    47221 accgctgcgt tatatgatta aacacggcta tctgacgccg ccagaacgac tggatatgcc
    47281 agtagtgcaa tacgatttca gccgcttgca ggcacagagt aacgggctat tcagcgaagc
    47341 cgatctcaac cgcgagctga aaaaacagca acgcattacc ccgcacatca tcagccagat
    47401 tatggagttt gctgtaacgc gcaaaggggt gatgattttc gccgccaccg ttgaacacgc
    47461 aaaagagatt gtgggactgc tgcctgccga agatgcggca ctgattactg gcgacacccc
    47521 cggcgctgag cgcgatgtgt taattgaaga ttttaaagcc cagcgttttc gctatctggt
    47581 caacgtcgcg gtactgacca ccggatttga cgccccgcac gtcgatctta tcgccattct
    47641 gcgccctacc gaatcagtga gtctttacca acaaattgtc gggcgcggtc tgcgtctcgc
    47701 tcccggcaag actgattgct taattcttga ttatgcgggt aaccctcacg atctctacgc
    47761 gccggaagtt ggtacaccga aaggcaaaag tgacaacgtt ccggtacagg ttttctgccc
    47821 tgcctgcggt tttgccaaca ccttttgggg gaaaacgacc gccgacggga cattgattga
    47881 acactttggt cgtcgctgtc agggatggtt tgaagatgac gacggacatc gcgaacagtg
    47941 tgacttccgt ttccgtttta aaaattgccc gcaatgtaac gctgaaaatg atattgccgc
    48001 ccgccgctgc cgcgaatgtg acacggtact ggttgatccg gatgatatgt taaaagcggc
    48061 gctacgactg aaagacgcgc tggtattacg ctgtagcggc atgtctttgc agcatgggca
    48121 cgacgagaaa ggcgaatggt tgaaaatcac ctattacgat gaagacggcg cggatgtgag
    48181 tgagcgtttc cgtctgcaaa cgcccgccca gcgtaccgcc ttcgaacaac tttttatccg
    48241 tccgcatacg cgcacaccgg gcatccctct gcgctggatc accgccgccg atatcctcgc
    48301 ccagcaagca ttactgcgac acccggattt tgtcgtcgcc cgcatgaaag gtcagtactg
    48361 gcaagtgcgt gaaaaagtgt tcgattacga aggtcgtttt cgtcgggcgc acgaattacg
    48421 cggttaatgg tagttttgat tgatgtataa ggcgattgag tatagaatct cgcccgcttt
    48481 tgcatacgca aagcagatca cttacctgtt gctgggtcgc ctgtagcagg attaatttaa
    48541 gagagaaaga aatgtttact atcaacgcag aagtacgtaa agagcagggt aagggtgcga
    48601 gccgccgcct gcgtgccgct aacaagttcc cggcaatcat ctacggtggc aaagaagcgc
    48661 cgctggctgt cgaactggat cacgacaaag tcatgaacat gcaagttaaa gctgaattct
    48721 acagcgaagt tctgaccatc gttgttgacg gtaaagaaat caaagttaaa gctcaggacg
    48781 tacagcgtca cccgtacaaa ccgaagctgc tgcacatcga cttcgttcgc gcttaattgc
    48841 tgaataagtt gtaaaaaacc ccgctccggc ggggtttttt gtctctgcaa attatgcctg
    48901 attacggtat tgctattttt tgcaggccag ataaggcgtt cacgccgcat ctggcattca
    48961 gaacacaaaa ccgattaatt gccgccagac gtccggcgct gcaattgatc gcgcaagttc
    49021 ggcggcgtgc ctttaatggt cagggtatcg gttgctggat cccagaagat gcgttcacct
    49081 aacagcatcg catcaaagtt aatagtcaag ccaccgccgc taccggcaaa cttcgtcaac
    49141 tgacgcagtg tgctacggtc tgccgggaag ctctcttcca gttcgtagcc tttttcagcc
    49201 gcaaactccg tgaagctcac ttcgctgacg cctgccagct ctttcgacag cgattccagt
    49261 tcaatctctt cacctgcctg cagctgctca ttacagtagc tataaacctg ctggcgcacg
    49321 ttctgtcgct ctgctttatc cagttgtgct tcggcagtga aatcatcgac cgcttgcaac
    49381 agcccacggt tttgcgcttt cgcgtttaac ccttcgctgg caccgaggaa atccataaag
    49441 aaatccgcca ctttgcgccc tactcgccct ttcaggaaag tgagatagcg ggtcgactct
    49501 ggattggttt cccactcggt taaatcgata cgcgcaacaa tatccgcatg attgatatca
    49561 agataatggg ttgggttgat gtcgagattt tcgttaacac gcatactgct caggttgctc
    49621 agaactgcca ccagcagata ctccaccgcc agatagcgat agtggcaaaa cagcacgaat
    49681 ccaccgtcag caaaaggata tttcgctaat tcgtcacgca ggcgaccggt tgccgcgcgg
    49741 ctaaatgcca gaaagtcctc ttcgccctga cgctgtaagc gcagcgtctg cgccagttca
    49801 ctctcttcgc taaacaagcc gtaagcttta tttttggcgc tatagacccg atgcagttct
    49861 gccaccatct caacgacggt ttccgtcggt tccagcaatg aatcgcgcaa caccagctca
    49921 aggttctgct catcacgctt gataagctgg tgcagagcaa tctggttgat atccagactc
    49981 atgataaact ctcctttaag accgggcggt attcaaccac cgccggttaa gcgacgcaat
    50041 aaaagatggc ggtcatttta cagaaggcgg cgtaacgaga atgatttaac gcataaaaaa
    50101 gcagaaaaaa aaccgttgct acggtaatat gttgcccttt catcaactaa ctgatttcga
    50161 tttatgccac aaatttcccg ctacagtgat gaacaggttg aacaattgct cgcagagctg
    50221 ctcaacgtac tggaaaaaca taaggctccg accgaccttt ccctgatggt gttaggcaac
    50281 atggtcacta accttatcaa caccagcatt gccccggccc aacgccaggc aattgccaac
    50341 tcttttgccc gcgccttaca gtcctctatc aacgaagaca aagcgcacta agggaaacag
    50401 ataacaggtt atggtaactc atcgtcagcg ctaccgtgaa aaagtctccc agatggtcag
    50461 ttgggggcac tggtttgcac tgttcaatat tctgctttcg ctcgtcattg gcagccgtta
    50521 cctgtttatc gccgactggc cgacaacgct tgctggtcgc atttattcct acgtaagcat
    50581 tatcggccat ttcagcttcc tggtgttcgc cacctacttg ctgatcctct tcccgctgac
    50641 ctttatcgtc ggctcccaga ggctgatgag gtttttgtcc gtcattctgg caacggcggg
    50701 aatgacgcta ttactgatcg atagcgaagt ctttactcgt ttccatctcc atcttaatcc
    50761 catcgtctgg caactggtta tcaacccaga cgaaaatgag atggcgcgcg actggcagct
    50821 gatgttcatc agcgtgccgg ttattttatt gcttgaactg gtgtttgcga cgtggagctg
    50881 gcaaaagcta cgcagcctga cgcgtcgtcg gcgcttcgcg cgcccgctgg ccgcattctt
    50941 atttatcgcc tttatcgcct cgcatgtggt ttatatctgg gccgatgcca acttctatcg
    51001 cccgatcacc atgcaacgcg ctaacctgcc gctttcgtac ccgatgacgg cgcgacgttt
    51061 tcttgagaag catggtctgc ttgatgcgca ggagtatcaa cgccgtctca ttgagcaagg
    51121 taatccagac gccgtttccg ttcagtatcc gttaagcgaa ctgcgctatc gcgatatggg
    51181 caccgggcag aatgtgctgt tgattactgt cgatggcctg aactactcac gcttcgagaa
    51241 gcagatgcct gcgctggcag gttttgctga gcaaaatatt tcgttcacgc gccatatgag
    51301 ctccggcaac actacagaca acggcatctt tggcctgttc tatggcatct cgccgagcta
    51361 tatggatggc attctgtcga cccgtacgcc tgcggcatta attactgcgc ttaatcagca
    51421 aggctatcag ctggggttat tctcgtcaga tggctttacc agcccgctgt atcggcaggc
    51481 attgttgtca gatttctcga tgccgagtgt acgcacccaa tccgacgaac agaccgccac
    51541 gcagtggatc aactggctgg gccgctacgc acaagaagat aaccgctggt tctcgtgggt
    51601 ctctttcaat ggcacaaaca ttgacgacag caatcagcag gcatttgcac ggaaatatag
    51661 ccgggcggca ggcaatgtcg atgaccagat caaccgcgtg ctcaatgcac tgcgtgattc
    51721 tggcaaactg gacaatacgg tggtgattat cactgccggg cggggtattc cgctgagcga
    51781 agaggaagaa acctttgact ggtcccacgg tcatctgcag gtgccattag tgattcactg
    51841 gccaggcacg ccggcgcagc gtattaatgc gctgactgat cataccgatc tgatgacgac
    51901 gctgatgcaa cgcctgctac atgtcagcac acctgccagc gaatattcgc aaggtcagga
    51961 tttgttcaac cctcaacgcc gccattactg ggttaccgcc gcggataacg atacgctggc
    52021 aattaccacc ccgaaaaaga cactggtgct gaacaataac ggtaaatacc gcacttacaa
    52081 cttacgtggt gaaagagtga aagatgaaaa accacagtta agtttgttat tgcaagtact
    52141 gacagacgag aagcgtttta tcgctaactg attaattata aatcagttag caaaatatct
    52201 tacttgcaat cggtgtggaa aacggtagta ttagcagcca cgagtcggca cgtagcgcag
    52261 cctggtagcg caccgtcatg gggtgtcggg ggtcggaggt tcaaatcctc tcgtgccgac
    52321 caaatcgccc accggatacc accggaatta acagttttta gacacgaaaa gcccgctaat
    52381 ccttgattgg cgggtttttt tatggttttt agtcaccgga catcaccgaa tttataagct
    52441 atcagccgga ctttttagtt atacgcttag ttatacgggc ttgcgtataa ctaaagccgt
    52501 ataactaaaa ccctgttttt tcgtgtggaa tcatatacat atggcgagaa aaacaccccc
    52561 attaaccacg gtgcagatca aagcagccag accagcggaa aaggaataca ccctacagga
    52621 cggcggaggg ctttttctcc tggtcaaacc gtccggatca aaactctggc gattttccta
    52681 ctaccggcca tcggacaaaa aaagaatatt gctgagtttt ggatcgcttg atgatgtttc
    52741 cctggctgat gccagaaaac gccgtagcga gtacagagcg ttaatcagtg ccggaactga
    52801 cccgcagggc catgagaaga aaaaacgaga ggcagaggcc cgaagacaag ggaacacgtt
    52861 cgaaaatgtg gcggcggcat ggtaccaggt gaaaatcagc cagaatctgg cccccaacac
    52921 gattaaagac atctggcgat cgctggataa atatgtattc ccgttcatcg gcaacacccc
    52981 aatagacacc ctgaccgctc gcaggttcgt tgaggtgctt acccccatca aggagcgcgg
    53041 caacctggaa acactcaaac gggttttaca gcgcgttaat gaggtaatgg attacgccgc
    53101 caacagtggg ctgattgatg ccaatccggc tatgaatgtg cgtaaggcgt tcccctcccc
    53161 tgtaaaaaaa catatgccaa caatccgccc cgaacagttg ccggagctta tgcaggcttt
    53221 atcagtatcg gcaacagaac ggcagaccag attactgatt gaatggcagt tactgaccgt
    53281 aacccgtccc gccgaagcgt catcaacgcg gtgggatgaa atcgacctgg acgcgcaacg
    53341 ctggacgata cccgccggac gcatgaagat gcgcagggat cacgttatcc cactttccgg
    53401 tcaggctatg gcggtgctgg aggccatgaa accaatcagc caccaccgcg attatgtttt
    53461 cccgagtctg aaagacccac ggcagccaat gaacagccag acagcaaacg cagcattacg
    53521 acgcatgggg ttcgctggcg tgctggtgtc tcatggatta cgcgccatat tcagtacagc
    53581 agcgaacgag gaaggattcg agccggacgt aatcgaggcc gcacttgccc acgtcgacac
    53641 taacgaagtg agacgggcat acaaccggag caactacata gaaaaacgca tcgtgcttat
    53701 gcgctggtgg ggcgaatttg tcgaggctgc ggcgacgggc gtaaccctcg ccagtggtaa
    53761 aaggggtatc cgagccgttt agctgtacag aaaaccagta aaaactacga aaaccattta
    53821 aaaccgtcgt ataattgcat cacatttaac gacgaggcct tgaaaaatga aactgttaag
    53881 atgcaaacaa atttcagatg caattgcgac gggctgcaac tggccctgat ggttcagcat
    53941 gaattttggt caacctacga tccggaggac agaacgacgg ccccatcaaa aaaacaggta
    54001 gtagatttcc tggtatcccg tggggcttcc agaaatctgg ctgtaagtat tgataaggtt
    54061 gtacgtccgg catccatgaa gaccggaggc aggccaaaaa aatggcggta acaatcctga
    54121 aagcggcaga aatgccgctt tttttataaa tccctttcaa atcatcaata taaaaaacgg
    54181 tgtataccgt ttaaaaacgg tgagtactgt ttttccctgc atccgatgat ttaccgtatt
    54241 tctcaccgga atacaccgga ttaatgaggt aaatcacgat ggaagcaatc aaaaaagtca
    54301 tctttcgcca ggaagtaaaa aaacttatcc acatcaaggc agacagcacg cttcaaagca
    54361 tgatcaacgc cggagaattt ccgcagggtt ttcgcgttgg tttacgccgt cgcggatggt
    54421 atgaggaaga cgtaatcgca tggatgaaag aacgtgagca ggaagcacgc ggaacggctg
    54481 cgtaatggtg tgaggcataa cacgatgaac ataacaaaaa gcgccccgtt gccggaacgc
    54541 ccttgtgaac aattaacctg ctgcgcctta tgtgtatgtg atcccaaaca taagcacggg
    54601 aatgatagcc gctatcaggc tggtgggcaa tgtaattacc ctttgggttc tatgccgcgt
    54661 tgctgtaatt ctttgcggat tattcgcttt atccaggcag caagagaatc atcgccgtca
    54721 tgttgttgtg ccttttccat cttttcccgt aagtcaggat ctaaacggaa ctggaacgga
    54781 ggattaccac gcctttcgtt tttgtgtgtt gacacgttaa ttacacccca tgtaatgtat
    54841 ttatgtgtgg tgacacatta catacaatca acacaaaaag caaagcccgc tggcgctatg
    54901 aacaccaacg ggcttctaac caaaccatta attgaggtaa agattatggc tataaaagag
    54961 catagcttac tctttataca cacacaaacg ccccggaaac ggaataatcc gtttcatagt
    55021 tcggttttgt gcactcatcc tggcggtcgg tttttgccgg atttaagccg ccgtgggtat
    55081 ttatcgacaa aatcttcaag ggcaaatttt tctggtggca ctaagcgcgg cgctggtggt
    55141 ggtatttttg ttctggttag ttcttcctca agtctggcgc aggcttctga ctgcctttgc
    55201 cggatgattt catcatcttg cgtttgcgtg tcttgtattg gtgatagtgt gttgttggtc
    55261 atgatactgc cctgtaaagc aatgcgccgg agtacctcac accacagcgc tgatagtttt
    55321 tatcctttgg gttctatacc gcgccgcttt agttcagtgc gccccaactc tttaagccag
    55381 ttggctaggc ttatgccgtc gctctgtgct tctttatcga attgctcttt tagctctgga
    55441 gaaattcgca ttctgaattg tggggattgc ccgtcccctt tagggctttt atcgcgtttg
    55501 attgttgaca agtggtcacc tattgaatta gcctttctat tgttaggtga ccaccttaac
    55561 acaagagcac ttaaaaaagc aaagccccgc aagtgtcatt accactcgca gggcttctaa
    55621 ccaacaacgt aaactaggag ccgttatggt tgctgtaaat catataccac accttgtaca
    55681 cacacaaacg gcctttgtgt ggcgttttct ggccctgaat atcggagaaa aaaaccagtt
    55741 gatcgccagt gtcgaaaata gctatgattc ccgcgcacct cataaaacgg gtgccgggat
    55801 tggcgtcctg ggcaaaactg aagcgcataa cacgcgcccc gcgtgttttt ttgtgccgca
    55861 tagtcacacc ttatcaatgg tgggctgtac gggggcggag caatccgcgc cggtttcttc
    55921 agtgtccggt tacgccaacc ctgtacagtc cgccaccagc gaaattggcg tttccggtgg
    55981 cggttatctt aaccacactg aggaggctgc caacatggct actatcccta cccttgtaca
    56041 ttctcaaacc gcctttctct ggcgctttat catctttggc gcgtcagaac atcaaatcat
    56101 ccacgtaacc gcctggacgg aacgcgaagc gcgtaacctt tgcccgtccg gttgtgttgc
    56161 tgtatttgcc gcccgtattc gccagggaga aacctatgca caataaaacc acaccggacg
    56221 cagccgccgc cgcgctcact acgctgatgc acgcgcttat tgatatttct gttattgctg
    56281 acagggcgca taagcacgcc accagtgaaa cagaatatgc cggggctttc gttcctcatt
    56341 cgctggcggt tatgcaactt agtgctgata tggctctgaa tgaggccaaa gctatcctga
    56401 ttgctgattg tgaaaatggg ggggggttat gcgtgatgat cgttttaatt ccctgaaaca
    56461 ggaattttcc ggctttcctg atgatgcggc tgatgcgctt tcgtcaatat ctgaaattat
    56521 gcgggtggct tttttctttc tttgcactga tgagcacaga gatacagggc taaatattct
    56581 tgatattgcc gctaactatg ctgatttcgt gacagaggtt attttaagaa aagcgacgga
    56641 cggggattaa tatgcgtgat atttaccacg aaacaatata ccgcgtattt cttgcacttt
    56701 ctcacagtga aaatatgatg gaaatattgc gcatatggct tgaaacactt ggcgacaatg
    56761 aacgcgacaa acaaaaatca agaattgcca cggcattaat aacgcttctt gagcctgtaa
    56821 taaatgagtt gcaggaaata gagacattac acgaccagta taaagcgccc cacaccggag
    56881 aataaaaata atgaaactta aatattctgg cttaactgcc agtggcaaca ctcaccctaa
    56941 atttacgcgc ggtgatattt accgcgacca gtacggcggc acggtaatga ttaagggcgt
    57001 ggcgggacgg tgcgtaactt accgccgtga aggttacgaa tatgattgcg tgatgcctgt
    57061 ttatcagttc cggcgtgatt tttctctggt acagaccgcg ccgcataacg tgcccaccag
    57121 caacgccagg gcacaggcaa acatccagaa gctgaaaacc atgattaacg gattcagggg
    57181 caagaaatga aaagcgcacc gaacttaaaa aaacagcctt acgacaagat gaccgaagtc
    57241 attatttttg cgggtagtga tgcctgggca catgcgaaac agtggcagga acaggacggg
    57301 cgactggctg gcgataatgt gcctcccgtt gtgctggctg atgatcaact ggatgaactg
    57361 gcagaccaga gaatcatcga cgaggggcgc tattgtgtcc ggctgtacaa ggcaggccac
    57421 atcaggccat caaatattaa tgccattgcg cacaagctgg cggcggcggg tgtaactgat
    57481 gcgaattatt accccgaagg gatgcacagc catatgcggg agaactggcg cgaatacctg
    57541 gaacgggtgc gcgggaaaga gccggcggaa gaaaaaaacc accagcgaaa aaccacgcta
    57601 ccgatgagcg ttggatctac cggatacgac acgcaactgg attacgtggt taaggggatt
    57661 attccggcgg tatcgctatg cagcatatac ggggctagcg ggtcctataa atcattcctt
    57721 gccggatcgt gggcgtgcca tgttgccacg ggccgccagt ggggaggccg cagggtggcg
    57781 catggcgctg ttctctatgt ggttggtgaa ggcggtatcg gcgttccgcg tcgtgtaaaa
    57841 gcctgggagg ttgtgcacgg tgagcaggtg aaaaatctgt atctggtaaa ccgtcccatc
    57901 tttccggcgg tcccgcttga tgttgatgaa atggttatcg ctgcccgtca ggtggagcgg
    57961 gaaacgggta aacctgtacg catgattatt ctggatacgc tggcgcgttg ctttggtggg
    58021 aatgatgaaa atgattcccg tgatatgggg gcgtttatcc gtggttgtga cgaactgaaa
    58081 cgacgcacag gggccacggt gctggtggtt caccattccg gcaaggatga gacgaaaggc
    58141 gcgcgcggtt ccagtgcatt tcgtgcttcg ctggatgctg aataccggat acgcagggag
    58201 gacgcaggaa gcgaagcgct ggttatctca tgcaccaaaa tgaaggacgc ggaggaactc
    58261 aaagaagccg catatgactt acgcgtggtg gagcttttta ccgacgctga cggtgaatta
    58321 atcacgtcgc tggtggtggt ggatgatccg cgccctcctg ttgaactgga gcgcatcgag
    58381 gaggcaggga acaagacgga aaaccatacc gcgctatggg ggtgcatccg ttcacgcaca
    58441 cagaacggcg acaagtgcac gatcccgctg ttacgtgatg acatgaaaaa gctggggtat
    58501 gaaatgaaaa acttccggcg ctggctgtac aagctggaaa aagatggggt tattcgtatc
    58561 gatggggatg atgtagcgcc gctataaaag tgaggagcaa aagcgagggg gatagaaaga
    58621 gggccaaaat tagcccgctc tccctcactt ttcgacctgt atacatcctc aaaagtgagg
    58681 ggtaaaaaaa tacttatgaa acacacacat agaaaaaccg aaaatcccaa ctgcgacgaa
    58741 gtgagacgct tgaaaaagtg aggcgaaaaa gtgagaggtt gcgagaaatg acccaaaaac
    58801 gcagagacag aacagagcca aaatataaag cgttagacat gactgagcac accttaaagg
    58861 tggcaatcag aacgatagac cgccacacgc gggaaggata cgcgaaggaa catcccgacc
    58921 tgataagcgc attcatgacc acggcggcgg caaactttgc cacgctgaca gaacgggaga
    58981 ttgccgaagc ggaacaggta acaaccatca acgttaaaac cggagaggtg gaatcatgac
    59041 agcacagata gcagcttacg ggcggctggt ggacgacccg caggtaaaac agaccagcaa
    59101 gggcacacca atgacgctgg cgcgtatggc ggtatctttg ccatgcagcc aggcacagga
    59161 cggacaggcg acgttatggt tatcggtcat cgcatttggc aaacaggccg acttcctggc
    59221 taaacatcaa aaaggcgatg ttgccagcgt atccggcacg atgcaggtaa gccagtggac
    59281 cggacagaac ggagaaacgc ggcagggctg gcaggttatc gcagacagcg taatcagtgc
    59341 ccgcgcggca cgtccaggcg ggaacagacg caaaaccaca ggcacacagg gtaatcagcc
    59401 accagcggga ggcgatgacc cttacggtga tgatatttcg ttctgagggg gtgacgatgg
    59461 tacatgaccg catagcggag gaactggagg cgaaaggctt ttaccggagg gcggcggcgc
    59521 gatggggtga agtcatgcag ctggtggaga cagacaagga acggcatcac atcacgatgc
    59581 gacggctgga atgttcaagg aaggcacaga gggcaccgga gccgccggat aatttcggag
    59641 acctgaaaaa ggcagtcgat cgcacttatg ccgaaatggg tatagatggt gctggtgatg
    59701 aaatatggcg caattaccag gacagctaat caaacagccg gagaaatccg gctttttttg
    59761 caccagttga aacggtatgg cgcattaccg ggttttcgtc acggtcaggc atagttacta
    59821 tctgaaacaa acagacacaa cagaggaaaa aaacaatgcc gatgaaattt gatgagatat
    59881 taaaacagcg tgataaatac catgctgaca acatggagac gatgagcatc aatgattacc
    59941 gcgcattcct ggagacgggc gcactgattg aaaaggatca gcatggtttt gtgagatgtg
    60001 ctctatccgg tgaaatgctg gcggtaaatc ctgaacagat agatgcattg atagaatttc
    60061 tgaaagagat cagagactga gccagcacac agcacacata gccggagcaa tccggctttt
    60121 ttgcgcccaa aaaaagcccg ataaggtcag agggttctta tcgggctttt gcatatgagg
    60181 tttttggatg cactggcgtt cgtgatcggg ataatcattt cataatttgc aacataactc
    60241 aatatcattg cataaaatgc aattctgatt ataatcagga ctggataaac atccagttat
    60301 gattttttta gtcgaagagg aatttcttac tatggctgaa gagaaaaaag gcggtgtttc
    60361 ggtgtacata agccccgaaa tcgtggaggt gctcaagcag cgccacaaaa aaaactatga
    60421 agctggcgtt gcggctggac tggatccgct gatgacgccg gagccgtcga taggttcact
    60481 tgtacgctct tatttacttg cggcgcttgg gatgcataaa aattatgggg gtgaataatg
    60541 gcaggcaaag caacggcact taacactaac cagcttttca tgtacctgaa tcgcggggat
    60601 attgcggatt ttaaattcag ccctctgttt accacgctgt ttttcccgaa cgtggcgaca
    60661 ttcagcacgc aaaacatcat gctggatacc ctggacattg aagaagtcac catgtcggcg
    60721 ttttgttcgc ctatggtggg tagccaggtt cagcgcgata aagggtacga aaccagcaca
    60781 atcaaacctg gctacatgaa gccaaagcac gaaatcgatc caacgaaaac catcatgcgc
    60841 atggctggag aagatccggc acagcttaac gaccctacct atcgccgtat gcgcctgatt
    60901 actggcaaca tgcgccgcca gataaacgcc attaaggcac gcgtggaatg gctggcggtg
    60961 aatgcggtaa cgaccggaaa aaacatcatt gagggcgaag gcatagaacg ctatgaaatc
    61021 gactggaaaa taccggaaaa ctgcatcata gagcaggcca agggtaaaaa atggtccgag
    61081 caggataaag acatgcacga cccaatctat gacatcgagc tttatgctga tcaggctggt
    61141 tgccccgcaa acgtcatgat tatgggcgct gaggtatggc gcacattacg cagctttaaa
    61201 aaattccgtg agctgtacga tctttcccgt ggttcagaat ccgccgccga actggcctgt
    61261 aaaaacctgg gcgaagtggt gagctttaaa ggctatcttg gtgatctggc ccttatcgtc
    61321 tattccggca aatacactga cagcgatggt accgaaaaat atttccttga gcctgatttg
    61381 ctggtcctgg gtaacaccaa caataaaggg ctggtggcct atggtgcgat aatggatcag
    61441 gaagcggtaa gaacgggcgc aacacaaaac atgttttacc cgaaaaactg gattgaggac
    61501 ggcgatccgg cgattgagta cgtgcagaca cacagtgcac cgcagccggt accggcagat
    61561 attcgcaaat ttgttaccgt caaaattggt taacgggggg attctatgga cactccatac
    61621 attgagttat ttgcaggcag tcagcaggta tccacgacgt tggtacattt tgccgctgat
    61681 gctggcgtta ttcaggaatt taccccgctg atgctggcag acaatggcga gtttaagccg
    61741 tgggatggtc aggaatctgg caaggctgtt tatctgacct cgtaccctgt ggacacgtca
    61801 aagcagaaat cagcacagtg ttacaagacg gggatattca atattgccgc cgttaactgg
    61861 cctgagagcg tcgacaccga tgcgaaaaaa tgcgccgcct ttgcgggttc cggcgtatcc
    61921 gttcagccgc tggcccgata agcaggggga acgatggcaa cgaatgaaag catcatgacg
    61981 ctaccgctgg cgagtaaatt taaagtcgaa gcgcgggcaa tggctgacag aggtttatca
    62041 acctatgagg ccgtatatca actaaacaaa ctggaagagc aggacaagcc gcgcgctgat
    62101 gcgattatgg cgcttcatga gcataaagac tatcagccgc tgttgcgtgc aatggcaaac
    62161 gtgccttgta tcgatgttgg tacggctaaa agcatcctta gcatgaccat agagcaggaa
    62221 cgtccgaagg ttgcgccgga acttaccgca gcctttgaaa actttatgga catgcacagc
    62281 ccgcaagccg tatcagctgg catggcatac gatggcagaa accagggcga tgacggcgac
    62341 atcgatcgca tactgaaaat catctgagac aaggccggag aaatccggct tttttacggg
    62401 tccttcctgg aattatggcc cgttacgggg cggcgacctc gcggtttttc gctatttatg
    62461 agccttttca ggggggtggt ggtggttttg ttgtttgctc tatctttatg aatgaaaagg
    62521 gaaagatgca aacaatacac caacctgaag cagtaattaa gttggtgtat taatgaaatc
    62581 gcacctgatg aacaaaaaaa acatggcgaa aagctgccgt gtaagtgcga cagcgttcga
    62641 caagtgggga gtgactcccg ttgaacgtaa aggccgtgag gcgttttatg atgttgccag
    62701 cgtgatagac aatagggtta acaatgcaat tagccagctt acaaacgaca aaggcgatat
    62761 tgatgatgct gaactcttac gagtcaggat cagattactg acagcacagg cggaggcgca
    62821 ggaacttaaa aacgagcgcg atcgcggtga cgtgattgat actgagtttt gcctgtacgc
    62881 gctttcaaag ctggcgagtc agatttcatc aatcatggac agcctcccgc ttactatgca
    62941 aaggagcttc ccacagattc cccccgccat gctggacagg cttaaaaggg aagtggttaa
    63001 agcctgtaat ggcagtgcca gagttgccga caacctccca cagatactgg ctgattactt
    63061 gaaagaaaca accggaaacg taccggaaaa gttgctacag aagaaaggcg agtaaccgca
    63121 gcgcaattat tgaacaaaac tgagaaacga catgaaagcg tcataaatcg ccat
//
LOCUS       DAZKGF010000007        59060 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZKGF010000007 DAZKGF010000000
VERSION     DAZKGF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.007"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..1397
                     /gene="proY"
                     /locus_tag="ACHOQV_000444"
     CDS             24..1397
                     /gene="proY"
                     /locus_tag="ACHOQV_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFP9628870.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     SLKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENH"
     gene            1553..3370
                     /gene="malZ"
                     /locus_tag="ACHOQV_000445"
     CDS             1553..3370
                     /gene="malZ"
                     /locus_tag="ACHOQV_000445"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFP9628871.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EETSVPMHKQRSQPQPGVTAWRAAIDLSGGLPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLYQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(3375..3956)
                     /gene="acpH"
                     /locus_tag="ACHOQV_000446"
     CDS             complement(3375..3956)
                     /gene="acpH"
                     /locus_tag="ACHOQV_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFP9628872.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     NSWYDLDAHYDALETCFWQFYPRMMEQASRKAL"
     gene            4049..5119
                     /gene="queA"
                     /locus_tag="ACHOQV_000447"
     CDS             4049..5119
                     /gene="queA"
                     /locus_tag="ACHOQV_000447"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP9628873.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDERINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            5174..6301
                     /gene="tgt"
                     /locus_tag="ACHOQV_000448"
     CDS             5174..6301
                     /gene="tgt"
                     /locus_tag="ACHOQV_000448"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP9628874.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            6324..6656
                     /gene="yajC"
                     /locus_tag="ACHOQV_000449"
     CDS             6324..6656
                     /gene="yajC"
                     /locus_tag="ACHOQV_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP9628875.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            6684..8531
                     /gene="secD"
                     /locus_tag="ACHOQV_000450"
     CDS             6684..8531
                     /gene="secD"
                     /locus_tag="ACHOQV_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFP9628876.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            8542..9513
                     /gene="secF"
                     /locus_tag="ACHOQV_000451"
     CDS             8542..9513
                     /gene="secF"
                     /locus_tag="ACHOQV_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFP9628877.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            9643..9990
                     /gene="yajD"
                     /locus_tag="ACHOQV_000452"
     CDS             9643..9990
                     /gene="yajD"
                     /locus_tag="ACHOQV_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFP9628878.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(10028..10912)
                     /gene="tsx"
                     /locus_tag="ACHOQV_000453"
     CDS             complement(10028..10912)
                     /gene="tsx"
                     /locus_tag="ACHOQV_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP9628879.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(11211..11750)
                     /gene="yajI"
                     /locus_tag="ACHOQV_000454"
     CDS             complement(11211..11750)
                     /gene="yajI"
                     /locus_tag="ACHOQV_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP9628880.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            11901..12350
                     /gene="nrdR"
                     /locus_tag="ACHOQV_000455"
     CDS             11901..12350
                     /gene="nrdR"
                     /locus_tag="ACHOQV_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP9628881.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            12354..13457
                     /gene="ribD"
                     /locus_tag="ACHOQV_000456"
     CDS             12354..13457
                     /gene="ribD"
                     /locus_tag="ACHOQV_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFP9628882.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            13546..14016
                     /gene="ribE"
                     /locus_tag="ACHOQV_000457"
     CDS             13546..14016
                     /gene="ribE"
                     /locus_tag="ACHOQV_000457"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFP9628883.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            14036..14455
                     /gene="nusB"
                     /locus_tag="ACHOQV_000458"
     CDS             14036..14455
                     /gene="nusB"
                     /locus_tag="ACHOQV_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP9628884.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            14533..15510
                     /gene="thiL"
                     /locus_tag="ACHOQV_000459"
     CDS             14533..15510
                     /gene="thiL"
                     /locus_tag="ACHOQV_000459"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFP9628885.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            15488..16006
                     /gene="pgpA"
                     /locus_tag="ACHOQV_000460"
     CDS             15488..16006
                     /gene="pgpA"
                     /locus_tag="ACHOQV_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP9628886.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDHNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(16060..17034)
                     /gene="yajO"
                     /locus_tag="ACHOQV_000461"
     CDS             complement(16060..17034)
                     /gene="yajO"
                     /locus_tag="ACHOQV_000461"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFP9628887.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLLRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESNENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(17089..18951)
                     /gene="dxs"
                     /locus_tag="ACHOQV_000462"
     CDS             complement(17089..18951)
                     /gene="dxs"
                     /locus_tag="ACHOQV_000462"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP9628888.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEIVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            complement(18976..19875)
                     /gene="ispA"
                     /locus_tag="ACHOQV_000463"
     CDS             complement(18976..19875)
                     /gene="ispA"
                     /locus_tag="ACHOQV_000463"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFP9628889.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLEALERIHRHKTGALIRAAVRLGALSAGDKGRRALSVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(19875..20117)
                     /gene="xseB"
                     /locus_tag="ACHOQV_000464"
     CDS             complement(19875..20117)
                     /gene="xseB"
                     /locus_tag="ACHOQV_000464"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP9628890.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            20323..21771
                     /gene="thiI"
                     /locus_tag="ACHOQV_000465"
     CDS             20323..21771
                     /gene="thiI"
                     /locus_tag="ACHOQV_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HFP9628891.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(21825..22415)
                     /gene="yajL"
                     /locus_tag="ACHOQV_000466"
     CDS             complement(21825..22415)
                     /gene="yajL"
                     /locus_tag="ACHOQV_000466"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFP9628892.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(22378..23289)
                     /gene="panE"
                     /locus_tag="ACHOQV_000467"
     CDS             complement(22378..23289)
                     /gene="panE"
                     /locus_tag="ACHOQV_000467"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9628893.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGITVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            23457..23948
                     /gene="yajQ"
                     /locus_tag="ACHOQV_000468"
     CDS             23457..23948
                     /gene="yajQ"
                     /locus_tag="ACHOQV_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFP9628894.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(24076..25440)
                     /gene="yajR"
                     /locus_tag="ACHOQV_000469"
     CDS             complement(24076..25440)
                     /gene="yajR"
                     /locus_tag="ACHOQV_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9628895.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILAATGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADTAANEALEVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(25589..26479)
                     /gene="cyoE"
                     /locus_tag="ACHOQV_000470"
     CDS             complement(25589..26479)
                     /gene="cyoE"
                     /locus_tag="ACHOQV_000470"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFP9628896.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(26491..26820)
                     /gene="cyoD"
                     /locus_tag="ACHOQV_000471"
     CDS             complement(26491..26820)
                     /gene="cyoD"
                     /locus_tag="ACHOQV_000471"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFP9628897.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(26820..27434)
                     /gene="cyoC"
                     /locus_tag="ACHOQV_000472"
     CDS             complement(26820..27434)
                     /gene="cyoC"
                     /locus_tag="ACHOQV_000472"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFP9628898.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKVFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVVSWL
                     ALTWLFGAGFIGMELYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(27424..29415)
                     /gene="cyoB"
                     /locus_tag="ACHOQV_000473"
     CDS             complement(27424..29415)
                     /gene="cyoB"
                     /locus_tag="ACHOQV_000473"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFP9628899.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(29437..30384)
                     /gene="cyoA"
                     /locus_tag="ACHOQV_000474"
     CDS             complement(29437..30384)
                     /gene="cyoA"
                     /locus_tag="ACHOQV_000474"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFP9628900.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(30844..32319)
                     /gene="ampG"
                     /locus_tag="ACHOQV_000475"
     CDS             complement(30844..32319)
                     /gene="ampG"
                     /locus_tag="ACHOQV_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFP9628901.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(32363..32941)
                     /gene="yajG"
                     /locus_tag="ACHOQV_000476"
     CDS             complement(32363..32941)
                     /gene="yajG"
                     /locus_tag="ACHOQV_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9628902.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            33246..33563
                     /gene="bolA"
                     /locus_tag="ACHOQV_000477"
     CDS             33246..33563
                     /gene="bolA"
                     /locus_tag="ACHOQV_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFP9628903.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            33908..35206
                     /gene="tig"
                     /locus_tag="ACHOQV_000478"
     CDS             33908..35206
                     /gene="tig"
                     /locus_tag="ACHOQV_000478"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9628904.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            35452..36075
                     /gene="clpP"
                     /locus_tag="ACHOQV_000479"
     CDS             35452..36075
                     /gene="clpP"
                     /locus_tag="ACHOQV_000479"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP9628905.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            36201..37475
                     /gene="clpX"
                     /locus_tag="ACHOQV_000480"
     CDS             36201..37475
                     /gene="clpX"
                     /locus_tag="ACHOQV_000480"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFP9628906.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            37663..40017
                     /gene="lon"
                     /locus_tag="ACHOQV_000481"
     CDS             37663..40017
                     /gene="lon"
                     /locus_tag="ACHOQV_000481"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFP9628907.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            40208..40498
                     /gene="hupB"
                     /locus_tag="ACHOQV_000482"
     CDS             40208..40498
                     /gene="hupB"
                     /locus_tag="ACHOQV_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFP9628908.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            40690..42561
                     /gene="ppiD"
                     /locus_tag="ACHOQV_000483"
     CDS             40690..42561
                     /gene="ppiD"
                     /locus_tag="ACHOQV_000483"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9628909.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVVQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKK
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            42712..43083
                     /gene="ybaV"
                     /locus_tag="ACHOQV_000484"
     CDS             42712..43083
                     /gene="ybaV"
                     /locus_tag="ACHOQV_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP9628910.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            43177..43575
                     /gene="fadM"
                     /locus_tag="ACHOQV_000485"
     CDS             43177..43575
                     /gene="fadM"
                     /locus_tag="ACHOQV_000485"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFP9628911.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(43627..44322)
                     /gene="queC"
                     /locus_tag="ACHOQV_000486"
     CDS             complement(43627..44322)
                     /gene="queC"
                     /locus_tag="ACHOQV_000486"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFP9628912.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLHHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(44387..46087)
                     /gene="ybaE"
                     /locus_tag="ACHOQV_000487"
     CDS             complement(44387..46087)
                     /gene="ybaE"
                     /locus_tag="ACHOQV_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFP9628913.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLLLLQLPARDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            46187..47005
                     /gene="cof"
                     /locus_tag="ACHOQV_000488"
     CDS             46187..47005
                     /gene="cof"
                     /locus_tag="ACHOQV_000488"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFP9628914.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGNVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            47158..47616
                     /gene="decR"
                     /locus_tag="ACHOQV_000489"
     CDS             47158..47616
                     /gene="decR"
                     /locus_tag="ACHOQV_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFP9628915.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            47646..49418
                     /gene="mdlA"
                     /locus_tag="ACHOQV_000490"
     CDS             47646..49418
                     /gene="mdlA"
                     /locus_tag="ACHOQV_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP9628916.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAEDAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            49411..51192
                     /gene="mdlB"
                     /locus_tag="ACHOQV_000491"
     CDS             49411..51192
                     /gene="mdlB"
                     /locus_tag="ACHOQV_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP9628917.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVIMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     EIVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDANTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            51373..51711
                     /gene="glnK"
                     /locus_tag="ACHOQV_000492"
     CDS             51373..51711
                     /gene="glnK"
                     /locus_tag="ACHOQV_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP9628918.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            51741..53027
                     /gene="amtB"
                     /locus_tag="ACHOQV_000493"
     CDS             51741..53027
                     /gene="amtB"
                     /locus_tag="ACHOQV_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFP9628919.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(53076..53936)
                     /gene="tesB"
                     /locus_tag="ACHOQV_000494"
     CDS             complement(53076..53936)
                     /gene="tesB"
                     /locus_tag="ACHOQV_000494"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFP9628920.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            54154..54723
                     /gene="ybaY"
                     /locus_tag="ACHOQV_000495"
     CDS             54154..54723
                     /gene="ybaY"
                     /locus_tag="ACHOQV_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFP9628921.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQPAI
                     QQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFVL
                     PFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPVQ
                     ASGGATATVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(54754..55065)
                     /locus_tag="ACHOQV_000496"
     CDS             complement(54754..55065)
                     /locus_tag="ACHOQV_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFP9628922.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            55228..55324
                     /gene="ffs"
                     /locus_tag="ACHOQV_000497"
     ncRNA           55228..55324
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHOQV_000497"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            55444..55797
                     /gene="ybaA"
                     /locus_tag="ACHOQV_000498"
     CDS             55444..55797
                     /gene="ybaA"
                     /locus_tag="ACHOQV_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFP9628923.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(55839..57389)
                     /gene="pdeB"
                     /locus_tag="ACHOQV_000499"
     CDS             complement(55839..57389)
                     /gene="pdeB"
                     /locus_tag="ACHOQV_000499"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFP9628924.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWSSTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSLFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(57553..58023)
                     /gene="ylaC"
                     /locus_tag="ACHOQV_000500"
     CDS             complement(57553..58023)
                     /gene="ylaC"
                     /locus_tag="ACHOQV_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFP9628925.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(58139..58690)
                     /gene="maa"
                     /locus_tag="ACHOQV_000501"
     CDS             complement(58139..58690)
                     /gene="maa"
                     /locus_tag="ACHOQV_000501"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFP9628926.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(58863..>59060)
                     /gene="hha"
                     /locus_tag="ACHOQV_000502"
     CDS             complement(58863..>59060)
                     /gene="hha"
                     /locus_tag="ACHOQV_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFP9628927.1"
                     /translation="KTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRL
                     AELTMNKLYDKIPSSVWKFIR"
BASE COUNT        14443 a        14622 c        15918 g        14077 t
ORIGIN      
        1 ttgtgttgat gggttaggaa ttgatggaaa gtaagaacaa gctaaagcgt gggctaagta
       61 cccgccacat acgctttatg gcactgggtt cagcaattgg caccgggttg ttttacggtt
      121 cggcagacgc catcaaaatg gccggtccga gcgtgttgtt ggcctatatt atcggtggtg
      181 tcgcggcgta tatcattatg cgcgcgctgg gggaaatgtc ggtacataac ccggccgcca
      241 gctctttctc gcgttatgcg caggaaaacc tcggcccgct ggctggttac attaccggct
      301 ggacctactg ctttgaaatc cttattgtcg ccatcgccga tgtgaccgct tttggtatct
      361 atatgggtgt ctggttcccg acggtgccgc actggatttg ggtactgagc gtggtgctga
      421 tcatttgcgc cgtaaacctg atgagcgtga aggtattcgg tgagctggag ttctggttct
      481 cgttctttaa agtcgccacc atcatcatca tgattgtcgc cggtttcggc atcatcatct
      541 gggggattgg caacggcggg caaccgaccg gtattcataa cctgtggagc aacggcggct
      601 tcttcagtaa cggctggctt gggatggtga tgtcgttgca aatggtgatg tttgcttacg
      661 gtgggatcga aattatcggg attaccgccg gtgaagcgaa agatcctgag aaatcgattc
      721 cgcgtgcgat taactccgtg ccgatgcgta ttctggtgtt ctacgtcggt acgctgttcg
      781 tcattatgtc tatctacccg tggaatcagg ttggcactgc cggtagcccg ttcgtactga
      841 cgttccagca tatgggcatt acctttgccg ccagcattct taactttgtt gtgctgaccg
      901 cttcgctgtc ggcaattaac agtgacgtat ttggcgtagg ccgtatgctc cacggtatgg
      961 cagagcaggg cagcgcacca aaaattttca gcaaaacctc gcgtcgcggt attccgtggg
     1021 ttacggtgct ggtgatgact accgcgctgc tgtttgctgt gtatctgaac tacatcatgc
     1081 cggaaaacgt cttcctggtg atcgcttcgc tggcaacctt cgccacggtg tgggtgtgga
     1141 ttatgatcct gctgtcgcaa atcgccttcc gtcgccgttt accgccagaa gaagttaagt
     1201 cgctgaaatt taaagtaccg ggtggggtag caaccaccat cggcggtttg attttcctgc
     1261 tctttattat cgggttgatt ggttatcacc cggatactcg tatctcgctg tatgtcggtt
     1321 tcgcgtggat tgttgtgctg ttgattggct ggatgtttaa gcgccgccac gatcgtcagc
     1381 tggctgaaaa ccactaatcc ttgcgttctg taagccggtt caggaggaaa tcctgatccg
     1441 gctattccga aagttatccg cccccgtcct cctcccccaa atatccttca gatgatgagt
     1501 gatcctgcat taggctatgg caaggtgatc agattttcat cacaggggaa ttatgatgtt
     1561 aaatgcatgg cacctgccgg tgcccccatt tgttaaacaa agcaaagatc aactgctcat
     1621 tacactgtgg ctgacgggcg aagacccacc gcagcgcatt atgctgcgta cagaacacga
     1681 caacgaagaa acgtcagtac cgatgcataa gcagcgcagt cagccgcagc ctggtgttac
     1741 cgcatggcgg gcggcgatcg atctctccgg cggactacct cggcggcgtt atagtttcaa
     1801 attactgtgg cacgatcgcc agcgttggtt tacaccgcag ggcttcagcc gaatgccgcc
     1861 ggcacgactg gagcagtttg ccgtcgatgt accggatatc ggcccacaat gggctgcgga
     1921 tcagattttt tatcagatct tccctgatcg tttcgcccgt agtcttcctc gtgaagctga
     1981 acaggatcat gtctattacc atcacgcagc cggacaagag atcatcttgc gtgactggga
     2041 tgaaccggtc acggcgcagg cgggcggatc aacgttctat ggcggcgatc tagacgggat
     2101 aagcgaaaaa ctgccgtatc tgaaaaaact tggcgtgacg gcgctgtatc tcaatccggt
     2161 gtttaaagct cccagcgtac ataaatacga taccgaggat tatcgccatg tcgatccgca
     2221 gtttggcggt gatggggcgt tgttgcgttt gcgacacaat acgcagcagc tgggaatgcg
     2281 gctggtgctg gacggcgtgt ttaaccacag tggcgattcc catgcctggt ttgacagaca
     2341 caatcgcggg acgggtggtg cttgtcacaa ccccgaatcg ccctggcgcg actggtactc
     2401 gtttagtgac gatggcacgg cgctcgactg gctgggctat gccagcttgc cgaaactgga
     2461 ttatcagtcg gaaagtctgg tgaatgaaat ttatcgtggg gaagacagta tcgtccgcca
     2521 ctggctgaaa gcgccgtgga atatggacgg ctggcggctg gatgtggtgc atatgctggg
     2581 ggaagcgggt ggggcgcgca ataatttgca gcacgtagcc gggatcaccg aagcggcgaa
     2641 agaaacccag ccggaagcgt atatcgttgg agagcatttt ggcgatgcac ggcaatggtt
     2701 acaggccgat gtggaagatg ccgccatgaa ctatcgcggc ttcacattcc cgttgtgggg
     2761 atttcttgcc aataccgaca tctcttacga tccgcagcaa attgacgccc aaacctgtat
     2821 ggcctggatg gataattacc gcgccggact ttctcatcaa cagcaactgc gtatgtttaa
     2881 tcagctcgat agccacgata ctgcgcgatt taaaacgctg ctcggtcggg atattgcgcg
     2941 cctgccgctg gcggtggtct ggttgttcac ctggcctggt gtaccgtgca tttattacgg
     3001 tgatgaagtg ggactggatg gtaaaaacga tccgttttgc cgtaaaccgt tcccctggca
     3061 ggtggaaaag caggatacgg cgttattcgc gctgtaccag cgaatgattg cgctgcgtaa
     3121 gaaaagtcag gcgctgcgtc gtggcggctg tcaggtactg tatgcggaag ataacgtggt
     3181 ggtatttgtc cgcgtgctgt atcagcagcg tgtactggtg gcaatcaacc gtggcgaagc
     3241 ctgtgaagtg gtgctacccg cgtcgccgtt gcttaatgtc gcgcaatggc aacgcaaaga
     3301 aggtcacggg caactgacag acgggattct ggctttgcct gccatttcgg ctacggtgtg
     3361 gatgaattaa cgtcttataa cgccttgcgt gacgcctgct ccatcatccg cggataaaac
     3421 tgccagaagc aggtttcgag agcatcgtaa tgggcgtcta aatcgtacca ggagttacgc
     3481 agggcatcca gacgtgggcg acggcttgcc atgccgttta acacgctctg gatgaaatcc
     3541 atatcgcgat agcgcaccaa ccactgctct gaccataagt agttgttcag attgataaag
     3601 cgtggcggtg agtctggaag aatagtcatc acttgctccc gggcataaca ggtaaattcc
     3661 tgtagcggaa aatcgggcga cagctgcgac cagtggcggg acagaaagtg atcccacatc
     3721 acatccagcg taataggcgc gacacggcgc gtttcattac gaaaccactc ccgcgcttcg
     3781 cggacttctg gcagattgtc ggttaatacg tcgattcgcc gatgcatatg aatgccagcc
     3841 acgacgtcgg gcggaaaact ttcttcagga tttccgcgta cgaaatcagc cagtaaattg
     3901 ccggaaagcg agctttccgc gagatgggct aaatgcaggt gagctaaaaa attcatcgat
     3961 tatattctat ccaaaggggg gtaaaggttg cagggagagc gccccggcac tagactaccc
     4021 gcctcttatt ttagtctgag tcagtgtcat gcgcgttacc gatttctctt ttgaattgcc
     4081 cgaatccctg attgcccact atcccatgcc tgaacgcagt agctgtcgtt tactgtcgct
     4141 ggacgggccg acgggcgcgc tgacgcacgg tactttcacc gatttactcg ataagctcaa
     4201 ccccggcgat cttctggttt ttaataatac ccgcgtgatc ccggcgcgtc tgtttgggcg
     4261 taaagccagc ggcggcaaga ttgaagtgct ggttgagcgg atgctcgacg acaaacgcat
     4321 tcttgcgcat attcgcgcct cgaaagcgcc aaaacctggc gcagaactgc tgctgggcga
     4381 tgacgaacgt attaacgcaa caatgaccgc gcgccacggc gcactgtttg aagtcgaatt
     4441 taatgatcaa cgttcggtgc tggatattct caacagcatc ggccatatgc cgctgccgcc
     4501 gtacatcgac cgtccggacg aagatgcaga ccgcgaactt tatcaaacgg tttatagcga
     4561 aaaacccggc gcggtagcag caccgaccgc cggactgcac tttgacgaac ctttgctgga
     4621 aaaattacgc gccaaaggcg tggagatggc gtttgtgacg ttgcacgttg gtgcgggcac
     4681 cttccagccg gtgcgcgtcg acaccattga agatcacatc atgcactcgg aatacgctga
     4741 agtgccgcag gatgtggtag acgcggtact ggcggcgaaa gcgcgcggta accgggtgat
     4801 tgcggttggt accacctcag tacgttcgct ggaaagtgcg gctcaggcag cgaaaaacga
     4861 tctcattgaa ccgttctttg acgataccca aatctttatc tatccgggtt tccagtacaa
     4921 agtggtcgat gcgctggtga cgaacttcca ccttccggaa tcgacgctga ttatgctggt
     4981 ttcggcattt gctggttatc aacacaccat gaatgcctat aaagcggcgg tagaagagaa
     5041 atatcgcttt tttagttacg gtgatgcgat gtttatcacg tacaatccgc aggcaattaa
     5101 tgagcgcgtt ggggagtaat tccgcggcgc tggtttaaac gttggactgt ttttctgacg
     5161 tagtggagaa aaaatgaaat ttgaactgga caccaccgac ggtcgcgcac gccgtggccg
     5221 cctggtcttt gatcgtggcg tagtggaaac gccttgtttt atgcctgttg gcacttacgg
     5281 caccgtaaaa gggatgacgc cggaagaagt tgaagccact ggcgcacaaa ttattctcgg
     5341 taacaccttc cacctgtggc tgcgcccggg ccaggaaatc atgaaactgc atggcgattt
     5401 acacgatttt atgcagtgga aagggccgat tcttaccgac tccggcggct tccaggtctt
     5461 cagccttggt gatattcgta aaatcaccga acagggcgtg cacttccgta acccgatcaa
     5521 cggcgacccg attttcctcg acccggaaaa gtcgatggag attcagtacg atcttggttc
     5581 ggatatcgtc atgatctttg atgagtgtac gccgtatcct gctgactggg attacgcaaa
     5641 acgctccatg gagatgtctc tgcgttgggc gaagcgtagc cgtgagcgtt ttgacagtct
     5701 cggaaacaaa aatgcactgt ttggtatcat tcagggcagc gtttacgaag atttacgtga
     5761 tatttctgtt aaaggtctgg tagatatcgg ttttgatggc tacgctgtcg gcggtctggc
     5821 tgtgggtgag ccgaaagcag atatgcaccg cattctggag catgtatgcc cgcaaattcc
     5881 ggcagacaaa ccgcgttacc tgatgggcgt tggtaaacca gaagacctgg ttgaaggcgt
     5941 gcgtcgcggt atcgatatgt ttgactgcgt aatgccaacc cgcaacgccc gaaatggtca
     6001 tttgttcgtg accgatggcg tggtgaaaat ccgcaatgcg aagtataaga gcgatactgg
     6061 cccactcgat cctgagtgtg attgctacac ctgtcgcaat tattcacgcg cttacttgca
     6121 tcatctcgac cgttgcaacg aaatattagg cgcgcgactc aataccattc ataaccttcg
     6181 ttactaccag cgtttgatgg cgggtttacg caaggctatt gaagagggta aattagagag
     6241 cttcgtaact gatttttacc agcgtcaggg gcgagaagta ccacctttga acgttgatta
     6301 atattaataa tgagggaaat ttaatgagct tttttatttc tgatgcggta gcggcaacgg
     6361 gtgcaccggc gcaaggtagc ccgatgtctt tgattttgat gctggtggta ttcggtctga
     6421 ttttctattt catgatcctg cgtccacagc agaagcgcac caaagaacac aaaaagctga
     6481 tggactccat cgctaaaggt gatgaagtgc tgacgaacgg tggcctggtc ggtcgcgtaa
     6541 ccaaagtagc ggaaaacggc tacattgcta tcgcgctgaa tgacaccact gaagtagtta
     6601 ttaaacgtga cttcgtagct gccgttctgc cgaaaggcac catgaaggcg ctgtaattaa
     6661 aatttttccc taagggaatt gccgtgttaa accgttatcc tttgtggaag tacgtcatgc
     6721 tgatcgtggt gattgtcatc ggtctgctgt atgcgcttcc caacctgttt ggtgaggatc
     6781 cggctgttca gatcactggt gcgcgcggag tcgccgccag tgagcaaacg ctgatccagg
     6841 tccagaaaac gttacaagaa gaaaaaataa ctgctaagtc tgtggcactg gaagagggcg
     6901 ctattcttgc gcgcttcgac tccactgaca cccagctgcg cgctcgtgaa gcattaatgg
     6961 gcgttatggg tgacaaatac gtcgtggcgc ttaaccttgc cccggcaacg ccgcgctggc
     7021 tggcagctat tcacgctgag ccgatgaagc tcggccttga cctgcgtggc ggcgttcact
     7081 tcctgatgga agtggatatg gacaccgcgc ttggcaaact ccaggaacaa aacatcgata
     7141 gcctgcgcag cgacctgcgc gaaaagggca tcccgtatac caccgttcgt aaagaaaaca
     7201 actacggcct gagcatcact ttccgcgatg ctaaagctcg tgatgaagcc attgcgtatc
     7261 tgagcaagcg ccatccggac ctggtgatta gcagccaggg cagcaaccag ctgcgtgcgg
     7321 taatgagcga tgctcgcctg agtgaagcgc gtgaatatgc ggtgcagcag aacattaata
     7381 tcctgcgtaa ccgtgtaaac caacttggcg tggcggagcc ggtggttcag cgtcagggtg
     7441 ctgaccgtat cgttgttgaa ctgccaggta ttcaggacac agcgcgtgcg aaagagattc
     7501 tgggtgcgac ggcaacgctg gaattccgtc tggtaaacac caacgttgat caggccgctg
     7561 cggcatccgg tcgcgtaccg ggcgactctg aagtgaaaca gacccgcgaa ggtcagccgg
     7621 ttgtgctgta caaacgcgta attctgaccg gtgaccatat caccgactcc acttccagcc
     7681 aggacgaata caaccagccg caggttaaca tctcgctcga tagcgcgggt ggtaacatca
     7741 tgtctaactt caccaaggac aacattggca aaccgatggc aaccctgttt gtggagtaca
     7801 aagacagcgg taagaaagat gcaaacggtc gtgcggttct ggtgaaacag gaagaggtga
     7861 ttaatatcgc caacatccag tctcgtctgg gtaacagctt ccgtattact ggcatcaata
     7921 acccaagcga agcgcgtcag ctttcactgc tgctgcgtgc cggtgcgttg atcgcgccga
     7981 ttcagattgt tgaagaacgt accattggcc caaccctggg tatgcagaac attgagcagg
     8041 ggctggaggc ttgccttgcc ggtctgctgg tttctattct gttcatgatc atcttctata
     8101 agaagtttgg tctgattgcg accagtgctc tgattgccaa cttgatcttg attgtcggta
     8161 ttatgtcgct gttgccaggc gcaacgctga gtatgccagg tatcgcgggt atcgtcttaa
     8221 cccttgcggt agcggtcgat gcgaacgtac tgatcaacga acgtattaaa gaagagttga
     8281 gcaacggacg tactgttcaa caggcaattg atgaaggtta tcgtggcgca ttcagttcta
     8341 tcttcgatgc gaacatcacc acgctgatta aagtcatcat cctgtacgca gtgggtaccg
     8401 gggcaattaa agggttcgcg attactaccg gtatcggtgt ggcgacgtcg atgtttaccg
     8461 cgattgtcgg tacgcgtgcc atcgtaaacc tgctatatgg cggcaagcgc gtcaagaagc
     8521 tgtcaatctg aggagtgcga tgtggcacag gaatatactg ttgaacaact aaaccacggc
     8581 cgtaaagtct atgactttat gcgctgggac tactgggctt ttggcatctc tggtctgctg
     8641 ttaatcgccg ctatcgttat tatgggcgtg cgcggcttta actgggggct ggatttcacc
     8701 ggtggtacgg ttattgaaat tacgctcgaa aaaccggctg aaattgacgt aatgcgtgat
     8761 gcattgcaaa aagccggttt tgaagagccg atgctgcaaa actttggtag cagccacgac
     8821 atcatggtcc gtatgccgcc tgctgaaggc gaaaccggcg gtcaggtgct gggcagccag
     8881 gtcctgaagg tgattaacga atccaccaat cagaatgcgg cagtgaagcg tattgagttc
     8941 gtcggtccga gcgtgggggc agaccttgcg caaaccggtg cgatggcgtt gatggcggcg
     9001 ctgctgtcta tcctcgtgta cgtaggtttc cgctttgagt ggcgactggc ggcaggggtt
     9061 gttattgcgc tggcacacga cgttatcatt acgctgggta ttttgtcgtt attccatatc
     9121 gagattgacc tgaccattgt ggcatcgttg atgtcagtta tcggttactc gcttaacgac
     9181 agtatcgtgg tatcggaccg tattcgtgaa aacttccgca agatccgtcg cggtacgcct
     9241 tacgaaatct ttaacgtgtc cttgacccag acgctgcacc gtaccttgat cacatccggt
     9301 actaccttga tggttatcct gatgctgtac ctcttcggtg gtccggtact ggaaggcttc
     9361 tcgctgacca tgcttatcgg tgtttccatc ggtactgcat cttccatcta tgtggcatcg
     9421 gcgctggctc tgaaactggg tatgaagcgc gaacacatgt tgcagcagaa agtggaaaaa
     9481 gaaggggcgg atcagccgtc aattctgcct taatcaagtt tcagctgatg ttgaaaatcc
     9541 cggtcagaag atcgggattt tttttgatgt aaggtgattg cgaaaatccc cgcatcttgg
     9601 gaaactgcgc gtaaccctac atttcatcca ggtaactttt tcatggctat catcccaaaa
     9661 aactatgcgc ggttagaaag cggctatcgc gaaaaagcat taaaaatcta tccgtgggtc
     9721 tgcggtcgct gttcccgcga gtttgtttat tccaacctgc gtgaacttac cgttcaccac
     9781 attgatcacg accatactaa taacccggaa gatggcagta actgggaatt actttgtctc
     9841 tattgccacg atcatgagca ttcgaaatac accgaagcgg atcagtatgg tacgaccgtt
     9901 atcgcagggg aagatgcgca gaaagatgtc ggtgaagcga agtacaaccc attcgctgat
     9961 ttgaaagcga tgatgaacaa gaagaagtga taaaaaatgc cggatatgaa atccggcatt
    10021 ctcacaatta gaagttgtaa cctactacca gataaccacc ccagccggta gagcgaacgt
    10081 tgaagttgcc gttgccgaag ttcagttctg catcgtcatt ccactgacca ccgttatgcc
    10141 agtaacgggc aacgacagag tagtgccagt gatcgtaatt cagcgccaga atatggctgg
    10201 acgcaataga gttattggta cgtgttttaa taccgttgtt cgcataaccg ctgtcatcgc
    10261 ccaggtcgga accccagtcg aagttggtga agccgatgta gctcagctgg ccgccccaca
    10321 gatcggtaat cggcacaaag tacttcactt tgaaacggta accgtcccac tcgttttcgt
    10381 tcgctgcgcc gtagttctgc cactggtatt tcgcatagac gttcatggac aggctcatcg
    10441 gcagaccagt atcgatgtcg gtacccagac ccatgtacca ggtgctctgg cggccatctt
    10501 tattacgacc catgtcgtaa atgtagttgt tcgcgaagta ccactctttg aacggaccaa
    10561 agctaaggtc agtattggtc agcttgtcga tggagaaacg cggttcgatt tccataaaca
    10621 gcggagaacc gtggttccag ataccttttg catcggagtt accgccgaag aataccggcg
    10681 catccgcata accatagaag tcgaaccagt cttttttagc gaatgcttcg tactcaaggt
    10741 aggtatcgtt gcggagctgc ggtccgaaac gggtgtgata gcttccgaca acgttaacgc
    10801 tctggtgcca ccagtcggaa agatactgcg gtttgtcgtt ttcagctgcg ttgacagtaa
    10861 aagacgaaga gagcgccagt accgcaccgg ctgccagtaa tgtttttttc atatgtatgc
    10921 cactgtttga aaatcccttg cgggagtgaa aaaggcgcaa attgcgtttc taaatatttc
    10981 gtatacagag gagcctatta taaaaatcat tgctcacaaa aatatgtttc gtttcacagt
    11041 tctatcattt acgtaatcga ttgcgttcac gtttacacac attcgggcgg ggattgtact
    11101 gactttcacc cttgttgcaa acttttacta tcaatccagg tgatgtaagc ggagtaacaa
    11161 aatgacggag aaagatgttg cgggacgcac aatatcgccc cgcaatacgt ttactgcata
    11221 acaggttgaa tgtcgtggat acgtacaaag cctaactgat ccacagacat accctttaac
    11281 tgtaacggaa tatcaacatc gctgggggcg aggacgctgg caggtgcgtt aatcaattga
    11341 ttctgcacat tgatttcctg aaagttgtct attgttccct gaatttgccc atattcaacg
    11401 gttccgctga atgcgggtaa agggtcattg gattcgccct gaatacgtag cgtcagggta
    11461 gtgccatctg tgtcaggcgt aatattcacc agcgacatac gtaaagtacc gatctggctt
    11521 tccagtcttg ctggtgtttt tgctccaggc agaaggtaaa ccccactgct ggattttgca
    11581 ttcagcttgt tttgctgagt gattttgacc gtttctttat tgagttgcgt catctcctgg
    11641 ttcagcgtgc tgacgctgtg tttcatctgt cgcacttcac tttgctgcac acaggcgcta
    11701 aggctgaaca ggcttcccag caggagcagt cgaaaaacgt tagtattcat aggtcgtccc
    11761 tttctcgtcg caattagcct aatggtagag ggtaaaagca gtgaaagcat cgatccttta
    11821 tctcaaaagc gttgcgcctt tgttgtatcg tcagttcagg gtaaaataga tttctgttaa
    11881 ccacctggtc aggacgccgt atgcattgcc cattctgttt cgccgtggac actaaggtaa
    11941 ttgactctcg tctcgtgggc gagggttcat ccgtacgccg ccgtcggcag tgtctggtgt
    12001 gtaatgaacg tttcaccacc tttgaagtgg cggagctggt tatgccgcgt gttgtaaaaa
    12061 gcaacgacgt gcgtgaaccg tttaatgaag agaaattacg tagcggaatg ctgcgggcgc
    12121 tggaaaaacg tccggtgagt tccgatgacg tcgaaatggc aatcaatcat ataaaatcgc
    12181 agctgcgcgc caccggtgag cgcgaagtgc cgagcaagat gattggcaat ctggtgatgg
    12241 agcaattgaa aaagctcgat aaagtcgcct atatccgttt tgcctctgtc taccgcagtt
    12301 tcgaagatat caaagaattt ggcgaagaga tcgcgcgcct ggaggactaa gccgtgcagg
    12361 acgagtatta catggcgcgg gcgctaaagc tggcgcaacg aggacgtttt accacgcatc
    12421 ccaacccgaa tgtcgggtgc gtcattgtca aagatggcga aattgtcggt gaaggttacc
    12481 atcaacgcgc gggtgaaccg catgccgagg tacacgcgtt gcgcatggcc ggcgaaaaag
    12541 ccaaaggcgc aacggcgtat gtcacactcg aaccctgtag ccatcatggt cgtacgccac
    12601 cgtgttgtga tgcattaatt gctgcgggcg tggcgcgcgt ggttgctgca atgcaagacc
    12661 ccaatccgca ggtcgctgga cgtggacttt accgtctgca acaggctggc attgacgtca
    12721 gccacgggct gatgatgagt gaagctgagc aattgaataa aggctttctc aaacggatgc
    12781 gcaccggctt tccttatatt cagttaaaac ttggcgcatc gcttgatggt cgcacggcga
    12841 tggcgagtgg cgaaagccag tggatcactt cacctcaggc gaggcgcgac gtacaacgac
    12901 tgcgcgcgca aagtcatgcc attttaacca gcagcgccac ggtgctggcg gatgatccag
    12961 ccttaacagt gcgttggtct gaactggatg aacaaactca gggactctac ccgcaacaaa
    13021 atctccgcca gccggtacgt attgtgattg atagccaaaa tcgcgtgacg ccggaacatc
    13081 gcattgtgca gcagcccggc gaaacctggt tcgcgcgtac gcaggaagat tctcgtgagt
    13141 ggccggaaac ggtgcgtacc ttgctgattc cagagcataa aggtcacctg gatctggttg
    13201 tactaatgat gcaactgggt aaacagcaga ttaacagcat ctgggtggaa gcggggccaa
    13261 cgctcgctgg cgcattgcta caggcgggtt tagtcgatga gctgattgtc tatatcgcac
    13321 ctaaactatt aggcaacgac gcccgtggat tatgcacgct gccagggctt gagaaattag
    13381 ccgacgctcc ccaatttaaa ttcaaagaga tacgtcatgt aggcccggat gtttgcctgc
    13441 atttagtggg tgcatgatct cccggctcga aagggaagca gcgcacgaaa tattatgcta
    13501 aaatccgccc ccctgcgggg ccatactcga acccgaagga agaaaatgaa cattattgaa
    13561 gctaacgttg ctaccccgga cgctcgcgtc gccatcacca tcgcgcgttt caacaacttt
    13621 atcaatgaca gcctgctgga aggtgcaatt gacgcactga aacgtatcgg tcaggtaaaa
    13681 gatgaaaaca ttaccgttgt ttgggtgcca ggtgcctatg agctgccgct ggcggcgggt
    13741 gcactggcta aaaccggtaa atacgacgcg gtgattgcgc tgggtacggt tattcgtggt
    13801 ggcactgccc actttgaata tgtcgctggt ggtgcaagca acggcctggc gcatgttgcc
    13861 caggacagcg aaattccggt tgcttttggg gttctgacca ctgaaagcat tgaacaagcg
    13921 atcgaacgtg ctggcaccaa agctggcaac aaaggtgcag aagctgcact gaccgcgctt
    13981 gaaatgatta atgtattgaa agccatcaag gcctgaaatt agtaagggga aatccgtgaa
    14041 acctgctgct cgtcgccgcg ctcgtgagtg tgccgtccag gcgctctact cctggcagtt
    14101 gtcccagaac gacatcgctg atgttgaata ccagttcctg gctgaacagg atgtaaaaga
    14161 cgttgacgtc ctgtacttcc gtgagctgct ggccggggtg gcgactaaca ccgcatacct
    14221 cgacggactg atgaagccgt acctgtcccg tctgctggaa gaactgggcc aggtagaaaa
    14281 agcagtactg cgcattgcgc tgtacgagct gtctaaacgt agcgatgtgc catacaaagt
    14341 ggccattaac gaagcgatcg aactggcgaa atcgttcggc gcagaagaca gccataagtt
    14401 cgtcaacggc gtactcgata aagcagcacc tgtgattcgc cctaacaaaa agtaatatcc
    14461 aggccggtag attcacggaa gaccgttcca tgatcgccgg ccttttcttt tttacctgct
    14521 gaggcataac gtatggcatg tggcgagttc tccctgattg cccgttattt tgaccgtgta
    14581 agaagttctc gtcttgatgt cgaactgggc atcggcgacg attgcgctct tctcaatatc
    14641 cccgagaaac agaccctggc gatcagcact gatacgctgg tggcgggcaa ccacttcctc
    14701 cctgatatcg atcctgctga tctggcttat aaagcactgg cggtgaacct aagcgatctg
    14761 gcagcgatgg gggccgatcc ggcctggctg acgctggcat taaccttacc ggacgtagac
    14821 gaagcgtggc ttgagtcctt cagcgacagc ttgtttgatc ttctcaatta ttacgatatg
    14881 caactcattg gcggcgatac cacgcgtggg ccattatcaa tgacgttggg tatccacggc
    14941 tttgttccga tgggacgagc cttaacgcgc tctggggcga aaccgggtga ctggatctat
    15001 gtgaccggta caccgggcga tagcgctgcc gggctggcga ttttgcaaaa ccgtttgcag
    15061 gttgccgatg ctaaagatgc ggactacttg atcaaacgtc atctccgtcc gtcgccgcgt
    15121 attttacagg ggcaggcact gcgcgatctg gcaaattcag ctatcgatct ctctgacggt
    15181 ctgatttccg atctcgggca tatcgtgaaa gccagcgact gcggcgcacg tattgacctg
    15241 gcattgctgc cgttttctga tgcgctttct cgccatgttg aaccggaaca ggcgctgcgt
    15301 tgggcgctct ctggtggtga agattacgag ttgtgtttca cggtgccgga actgaaccgt
    15361 ggcgcgctgg atgtggctct cggacacctg ggcgtaccgt ttacctgtat cgggcaaatg
    15421 accgccgata tcgaagggct ttgttttatt cgtgacggcg aacctgtcac gttagactgg
    15481 aaaggatatg accattttgc cacgccataa agatgtcgcg aaaagtcgcc tgaaaatgag
    15541 taatccgtgg catctgcttg ctgtcggatt cggaagtgga ttaagcccga tcgttcctgg
    15601 cacgatgggg tcgctggcgg cgattccctt ctggtatctg atgacttttt tgccctggca
    15661 gctctattcg ctggtggtaa tgctggggat ctgtatcggc gtctatcttt gtcatcaaac
    15721 ggcgaaagac atgggcgtgc acgatcacgg cagtattgtc tgggacgaat ttattggtat
    15781 gtggattacg ctcatggcgc tgccgaccaa tgactggcag tgggttaccg ccggatttgt
    15841 gattttccgt attctggata tgtggaagcc gtggccgatc cgctggtttg atcacaatgt
    15901 acatggcggc atggggatca tgattgacga tattgtcgcc ggggtgatct ccgctggcat
    15961 cctgtatttt atcggtcatc actggccgct gggtattctg tcgtagttgt gcaccgatgc
    16021 ctgatgtgac gcttgtcacg tctcatcagg cctgcatacc tatttaaatc ctacgaccgg
    16081 atgcggttta tacggcgttt ccagctcggc gatctgttcc ggcttcaagg tgatatccac
    16141 cgcgtttagc agttcatcaa gctgttcctc ccgagaagtt ccgataatcg gtgcggcaat
    16201 gcccggttta ctcaacaacc aggccagcgc aacttgtgct cgcgttgcac caagttcttc
    16261 actgacgccc gttaagcgtt ctgcaatttg tgcgtcattt tcattgcttt cttggtagag
    16321 atttttcccc acttcatccg acaccagccg tgcggtggtt tctccccaag gacgcgtcag
    16381 acggccccgc gccagcggac tccatggaat caccgccacg ccttcctgat aacacagcgg
    16441 cagcatctcg cgctcttctt cacgataaat cagattgtag tgatcctgca tactgacaaa
    16501 ctgcgcccag ccgtgctgtt tttgtagttc cagtgcctga gcaaactgcg aagcgtgcat
    16561 ggatgacgcg ccgatataac gtgctttccc ggcttttacc acgtcgttca gggcttccag
    16621 cgtctcttcg atcggcgtgt tgtaatccca gcgatgaatt tgcaggatat cgacataatc
    16681 catgccgaga cggagcaggc tgtcgtcgat agagcgcaaa atttgtgcac gggataatcc
    16741 ttccggtaaa tcaccaacgc gatggaacac tttggtcgca acgaccacgt cttcacgacg
    16801 ggcgaaatcc cgcagtgcgc gaccgacgat ctcttcgctg ctgccatcgg aatagctatt
    16861 ggcggtatca aagaaattta tgccgccttc cagtgcgcgt ttaatgatgg gacggctgct
    16921 ctcttccggc agtgtccatg cgtgattacc gcgatctggc tcgccaaagg tcatacagcc
    16981 gaggcaaagt cgggaaacac gaaggtcggt ttttcctaag gggttgtatt gcatgctgcc
    17041 actcctgcca tgaataattt cttaagcata gcaggagtag agtagggatt atgccagcca
    17101 ggctttgatt ttggcttcca taccggtggc atcgaggccg agttcggcgc gcatttcttc
    17161 ctgagttcct tgtggaataa agaagtccgg cagaccaatg ttcagcacgg gtactggttt
    17221 acgatgggcc atcagcactt cgttcacgcc gctgcctgca ccacccataa tggcgttttc
    17281 ttctacggta accagcgctt catggctggc ggccatttcc agaattaacg cttcatcaag
    17341 cggtttcacg aaacgcatat cgaccagcgt agcgttcagc gattcagcga ctttcgccgc
    17401 ttctggcatc agcgtaccaa agttaaggat cgccagtttc tcaccacgac gcttcacaat
    17461 gcctttgcca attggcagtt tttccagtgg cgtcagttcc acgccaactg cgttgccgcg
    17521 cgggtagcgc accgcggacg ggccatcgtt atagtgatag ccggtataga gcatctggcg
    17581 acattcgttt tcatcgctcg gggtcataat gacaatttcc ggtatgcagc gcaggtaaga
    17641 gagatcaaaa gcgccctggt gggtttgacc gtcagcacca acaatgcccg cacggtcgat
    17701 ggcgaacagg accggaagct tttgaatcgc cacgtcatgc agcacctgat cataggcgcg
    17761 ttgcaggaag gtggagtaga tcgcaacaat gggtttgtag cctccaatcg ccagacccgc
    17821 agcaaaggtc actgcgtgtt gctcggcaat tgccacgtcg aagtagcgat ccgggaattt
    17881 acgtgaaaac tcgaccatgc cggaaccttc acgcatcgcc ggagtaatcg ccatcagctt
    17941 gttatctttc gctgcggttt cgcacaacca gtcgccaaag atttttgaat agcttggcaa
    18001 accgccgcta cttttcggca gacaaccgct ggagggatca aatttaggca cggcgtggaa
    18061 ggtgattggg tctttttctg ccggttcata accacgacct tttttggtca tgatatgcag
    18121 gaactgcggg cctttcaggt cgcgcatgtt cttaagcgtg gtgataagcc ccagcacatc
    18181 gtgaccgtcc accgggccga tgtagttaaa gcccagctct tcaaacaacg tgccaggcac
    18241 taccatgcct ttaatatgtt cttcggtacg tttgagcagc tctttaatgg gtggcacgcc
    18301 agagaaaact ttttttccgc cttcgcgcag tgaagagtaa agcttaccgg aaagcagctg
    18361 cgccagatgg ttgttgagcg cgccaacatt ttcggaaatc gacatttcat tgtcgttgag
    18421 aaccaccagc atatcaggac ggatatcgcc cgcgtgattc atcgcttcaa acgccatgcc
    18481 agcagtaatc gcgccatcgc caatgacaca gacggtgcgg cgatttttgc cttctttctc
    18541 ggcagcaact gcgatcccaa ttccggcact gatggaggtt gatgaatgcc cgacgcttaa
    18601 tacgtcatat tcgctttcgc cgcgccacgg gaacgggtgc aggccacctt tctgacggat
    18661 ggtgccgatt ttgtcgcggc gcccggtcaa aattttatgc ggataagcct gatgccccac
    18721 atcccagatt aactggtcaa acggggtatt gtagacatag tgcagcgcca cggtcagttc
    18781 gaccgtgccc agcccggagg cgaagtgccc gctggaacgg ctcacgctgt cgagtaaata
    18841 gcggcgcagt tcgtcgcaga gtttcggtaa actctctttc ggcaacagac gtaactcctg
    18901 ggtggagtcg accagtgcca gggtcgggta tttggcaata tcaaaactca tcaggggcct
    18961 attaatactt attgtttatt tattacgctg gatgatgtag tccgctagcg cttccagtgc
    19021 cgaggtatcg agtgactgtt cagccagttg tttcagcgac tgacgggcat cgtcgatcag
    19081 atcccgggct ttcttccggg cttgctcaag acccagaagt gcagggtagg tacttttacc
    19141 aagttgctgg tcggcaccct ggcgttttcc caacgttgca gtatctccca ccacatccag
    19201 gatgtcatcc tgaacctgga aggcaaggcc gatgctctct gcgtacttgt cgagtacaga
    19261 cagagcacga cgtcctttat ctccggcgct taatgcacca aggcgaacgg cggcgcgaat
    19321 caatgcgccg gttttatgac gatgaatacg ctcaagcgcc tccagaggta cgtgtttgcc
    19381 ttccgcgtct aaatctaatg cctgaccacc gcacattccg gcaataccgc tggcgctcgc
    19441 cagttcagaa atcatcgaaa ttctgtcgcg atccgacact tccggcatat cagcatcgct
    19501 taaaatcgag aacgccagcg tttgtaaagc atcgccagcg agaatcgcgt tcgcttcgcc
    19561 aaacttcaca tggcaggtcg gcaaaccgcg acgcagatcg tcatcatcca tcgccggtaa
    19621 atcatcatga attaatgagt aagcgtggat acactcaacg gcggcagcgg gtgcgtccag
    19681 cgtgtttgtg ctaacgccga acatatgacc ggtggcataa accaggaaag gtcgcaggcg
    19741 cttaccacct aataatgcgc catactgcat ggtttcgacc acgggagtgt tctgaaaggg
    19801 cagtggggcg ataaaacggc tcagcgcctg gttggcctgc ttaacgcagg cttcgagttg
    19861 ctgcggaaag tccattactc attgtccggt gtaaaagggg ttagagaggc gtcttcattg
    19921 tcagacagca gaatttgcac gcgctgttcg gcttgttgta atttggcctg cccctgacgt
    19981 gccagttgca cgccgcgttc gaactcgttc agcgcctctt ccagcggcag gtcgccactt
    20041 tccagacggg ttacaatctg ttccagctcg ctcagcgcct tttcaaagct ggcgggcgcc
    20101 tcatttttct tcggcataat gaatgtctga ctctcaatat ttttcgcccc gtcatggtaa
    20161 cggactcagg gcaaatagca aataacgcgc aatggtaagg tgatgtgcgc agcaaagcga
    20221 tgttagtggt atacttccgc gcctggatgc agccgcaggt gtgggctgct gtatttttcc
    20281 ctatacaagt cgcttaaggc ttgccaacga accattgccg ccatgaagtt tatcattaaa
    20341 ttgttcccgg aaatcaccat caaaagccaa tctgtgcgct tgcgctttat aaaaatcctt
    20401 accgggaaca ttcgtaacgt tttaaagcac tatgatgaga cgctcgctgt cgtccgccac
    20461 tgggataaca tcgaagttcg cgcaaaagat gaaaaccagc gtctggctat tcgcgacgcc
    20521 ctgacccgta ttccgggcat ccaccatatt ctcgaagtcg aagacgtgcc gtttaccgac
    20581 atgcacgata ttttcgagaa agcgttggtt cagtatcgcg atcagctgga aggcaaaacc
    20641 ttctgcgtgc gcgtgaagcg ccgtggcaaa catgatttta gctcgattga tgtggaacgt
    20701 tacgtcggcg gcggtttaaa tcagcatatt gagtccgcgc gcgtgaagct gaccaatccg
    20761 gatgtgactg tccatctgga agtggaagac gatcgtctcc tgctgattaa aggtcgctac
    20821 gaaggtattg gcggtttccc gattggtacc caggaagatg tgctgtcgct catttccggt
    20881 ggttttgact ccggcgtttc cagttatatg ttgatgcgtc gcggatgtcg tgtgcattac
    20941 tgcttcttta accttggcgg cgcggcgcat gaaattggcg tgcgtcaggt ggcgcattat
    21001 ctgtggaacc gctttggcag ctcccaccgc gtgcgttttg tcgctattaa tttcgaacct
    21061 gtcgtcgggg aaattctcga gaaaatcgac gacggtcaga tgggtgttat cctcaaacgt
    21121 atgatggtgc gtgccgcgtc taaagtggct gaacgttacg gtgtacaggc gctggtcact
    21181 ggcgaagcac tgggccaggt gtccagccag acgctgacta acctgcgcct gattgataac
    21241 gtctccgaca cgctgatcct gcgtccgctg atttcttacg acaaagagca catcatcaac
    21301 ctggctcgcc agattggcac tgaagacttt gcccgcacga tgcccgaata ttgcggtgtg
    21361 atctccaaaa gcccgacggt gaaagcggtt aaatcgaaga ttgaagcgga agaagagaag
    21421 ttcgacttca gcattctcga taaagtggtt gaggaagcga ataacgttga tatccgcgaa
    21481 atcgcccagc agaccgagca ggaagtggtg gaagtggaaa ccgtcaatgg cttcggcccg
    21541 aacgacgtga tcctcgatat ccgttctatc gatgaacagg aagataagcc gctgaaagtc
    21601 gaagggattg atgtggtttc tctgccgttc tataaactga gcaccaaatt tggcgatctc
    21661 gaccagaaca aaacctggct gttgtggtgt gagcgcgggg tgatgagccg cctgcaggcg
    21721 ctctatctgc gcgagcaggg ctttaacaat gtgaaggtat atcgcccgta atttgtggtt
    21781 tttacgtcgc atctggtcag atgcgacgtt tgccgcatcc gccactactc gtaataatta
    21841 taaatccctg ccgccatcac cagttgtgat gccacttcat gggctttttc acgcccaacc
    21901 agcaggtcga taattttcag accaaagtcg atagctgtac ccggcccctg actggtcagc
    21961 aattttaccc gtgcatccca gacgacgcgc ttgtcctgcc attgttcggc gggtattttg
    22021 tctttcagcg tcgggaagcc ggtcatattg ccaatcggga agatatcgtg cgggaccagc
    22081 acggtggctg gtgcggcgca aatagccgcg acgatacgcc cggaacggtg gaactgttta
    22141 acggtttcaa ccagcagggt gctatcgcga aaacactctg cgcctttaat gcctccaggc
    22201 agcacgatca cgtcatattc gccatcagct acttcgacca gcggcgcatc cgccagcagc
    22261 ttcacgccgc gcgagcaggt aatcgccagg ttgccatcgc tggcgacgct ggcagtggtg
    22321 actttgatac cgccgcgaac cagcagatcg atagtggtga cggcttcagt ctcttcacta
    22381 ccaggggcga ggcaaaccag tgccgatgcg ctcatattca ctctcctttc tttttaccat
    22441 ttcaaacagg cgggtgtttt ccggtacggt aatcccatgc gcgcgggcgc ggcgtaagag
    22501 aaaaccattg atatagtcga tttcggtgtg gcgcagcgcg cggatatcct gcaacatcga
    22561 cgagatattt tccgctgtgg catcaatcac ctgcatcacg taatcacgca aatcttctgc
    22621 tgaagtatga tgtccttcgc gttcgatcac tgccgcgact tcctcgcata tctgcataat
    22681 ttcttgcgga tgatgacgta attcaccgtt cgggcaattc cagatggcag tcagtggatt
    22741 aatcacacag ttgactgcca gcttgcgcca cagctcggcg cgaatattgt tatgccaggc
    22801 aacgtcaggc aacacggttt gcaaaatatc cgccagataa ctgtaatcgc catcctgttg
    22861 ccgtgccgga ccaatatgcg tgataccgtt tgccacatga ataatgacat tgccgtcgcg
    22921 gcgagcggca tgggtggtgg tgcccatcag taatggctgc tgaatgtttt gcaactcttc
    22981 gatggtgccc atgccgttgt gaattaacag tattggcgta gttacaggca gtgtggacgc
    23041 gaggcttttg acggcatcgg aaacctgcca tgctttcagc gtcaccagga gcagatcgct
    23101 ggtggcgaga aaatcgggat cgttggcggt cagcgattcg ttaaatatcg aaccatctgt
    23161 ctcaaccaga ttcacgctac aataaggttg cggtacgcgc agccagccct gaacgtcgtg
    23221 accctgttta caaagtgctg taagccataa ttgccctaag gcaccgcatc ccaatacggt
    23281 aattttcatt gttcctcctc acccgcaacc actccgggtg ttcaataagg ctatccctta
    23341 attgtgcatg ctgttgcgac tatgcacaat taagggatac gtcctggtgc aggactgttg
    23401 gttatttaac tttgcaggta ttatgcttcg catcaaaaat gaagggagag gaaaagatgc
    23461 catctttcga tattgtctct gaagttgatc ttcaggaagc acgtaacgcg gtcgataacg
    23521 cgagccgcga agtggagtcc cgttttgact tccgtaatgt tgaagcctca tttgagctga
    23581 acgacgccag caaaaccatc aaagtgttga gcgagtccga tttccaggtc aatcagttgc
    23641 tggatattct gcgtgccaag ctgctgaagc gcggcattga aggcagttcg ctggatgtac
    23701 cggaaaatat cgttcatagc ggtaaaacct ggtttgtgga agcgaaactg aaacagggca
    23761 ttgagagcgc gactcagaag aaaatcgtca aaatgatcaa agacagcaaa ctgaaagtgc
    23821 aggcgcaaat tcagggcgat gagatccgcg taacgggtaa atctcgtgat gatttgcagg
    23881 ctgtcatggc gatggtacgt ggtggcgatc tcggtcagcc gttccagttc aaaaacttcc
    23941 gcgattaatc gcgacaacct gcgctttgtt catgtcagat gcggcatgaa cgcctgattt
    24001 ggcttacaaa ggtttgtaac tcaggaactt agtaggcctg ataagcgtag cgcagcaggc
    24061 aatttcgcct cgcttttatg cctggcgaat tgcctgttca acctcaaagc gattcgtcac
    24121 tttgctgtcg attttcacat acgctgaatg ttcttcttct gcaatcaaca cttctttgac
    24181 gccttcagtt tccagcaaac gtacttctaa cgcttcgttt gcggcagtgt ccgccgggat
    24241 ttcaatgcgc aagctgctga catacggcgg ttctttcatg gtactggcga ctgccagcca
    24301 cactgcggcc agcattgcgc cagcgagaaa tactccctga ccgtcaaaca tgccgtcaat
    24361 ccagccgccc agcgaaccgc caatcgccac gccaagaaac tggctggtgg agtaaacacc
    24421 catcgccgta cctttgtaac ctgctggcga ttctttactg ataagtgaag gcagaagggc
    24481 ttccatcaaa ttaaacgcca caaagaaaag ctgcacgccg accaccagtt gccagaactg
    24541 cgtttgtgcg ttccacaaca caatttccgc aaccacgatc agcccgacgc aaaagacaaa
    24601 gacttgcttc attttgcgct taacttcagc gtagataatg aaaggcacga ccgagccaaa
    24661 ggcgattaac atcgttgcca ggtagacctt ccagtgttca gccgccggga accccgcatc
    24721 agccatctgt ccgggcaggg caacaaaggt cgacatcagc aaaatatgca gacacataat
    24781 gccaaagttg agtttcagca gccgcggttc cgccagcact ttactgaaac ttcctttcac
    24841 cattccggac tcacgattaa gtacgtgagt gctactgttg ggcacaaccc aaatggtcaa
    24901 cgcaatgccg gttgctgcca gaatagcgat catccagaac agcgcgtgca gcccaagttt
    24961 gtgagtgatg atcgggccaa gcaccatcgc aatggcaaag gtaatgccaa agctcacgcc
    25021 gataaacgcc atcgctttgg tgcggttttg ttcgcgcgtg agatcggaaa gcagcgccat
    25081 aacggcagcg gcaatcgcac cggagccttg tagcgcccgg cccagaataa ttccccagat
    25141 ggagtcagag agcgcagcga taacgctacc ggcggcaaac actgccagcc caccgacaat
    25201 taatggtttg cgaccaatgc ggtcggaaag caggccaaac ggaatctgaa aaacggcctg
    25261 agtcagacca taaataccaa tggcaatacc gattaatgct tcgctggcac cttgcagagc
    25321 catgccgtac gtggtcagaa ccggcagaac catgaacatg cccagcatgc gcaacgagaa
    25381 tacggtccct aaaccccagg tcgcgcgtcg ctcacctggc gtcattttat aatcgttcat
    25441 taccacctct gttttaaatt cgcgactagt gtaaagcggc aaacgaatgg ggtaaacatg
    25501 cggatttttt agtaaatatt tcgagaggga tatgattcta atgctaagaa aaaaggtgcc
    25561 gtagcacctt tttaactgag aggttgtgtt accacacagc agccagcagc gtatgcgagt
    25621 ccggcaccat aaaatcaacg gacatcatca ccgagagggc agtgatggcg atgatagaga
    25681 agccgaacag cttgcgcgcc cagattctgt catcagcaac tttataaccg cgcagagcca
    25741 tgcctaacca ccagacgctc accgccgcgg cgactaccag atatttatac ccagcgtaac
    25801 cgccaagaga gagcatcagc gtggcaacgg caaaggcgat gatatacagc gtgatgtgat
    25861 tcttcgccac cgaaatgcct tttaccactg gcaataccgg aatgtttgcc gcctggtaat
    25921 ccttaaagcg gaaaatggcg atggcatagg agtgaggcat ctgccacaga ctgaagatag
    25981 caagcaggat cgctgcgcca ctatcgaact cgccggttac tgcacagtag ccgatcaccg
    26041 gcggcgcagc gccggagagc gaaccaatca atgtgccgta gactgagtgg cgtttcatgt
    26101 acaggctata aacgccgaca taaaccacaa agcccatcac ccccagccag caggccagcg
    26161 gattcgcgcc aaaccacagc agcataaagc cagcaatacc cagcaaggtg gcgtacacca
    26221 gcgagacagc aggtgagatc aggcctttaa ccagcacccg attcttcgtc ctttccatct
    26281 ttctgtcgat atccctgtcg atgtagttgt taaacacaca acccgacgcc acaaccagtg
    26341 acaccccaac cagcgtgtag ataaacaggg gataatcaat gctgcccttt gaggccaaca
    26401 ggaatccccc aatcaccgag atcaggttgc caaagatgat gcctggtttc gttacttgca
    26461 ggtattgctt aaacatcata accgccgctc ttagtgcatc atcatgttgt agttgaggtt
    26521 ccacataatc cagatggagc ctacaaccag gatagcgatg attagcacgg tgaagacaaa
    26581 cgccgtcatg ttccagcctt catctgattt ggtattcatg tgcaggaagc acaccagatg
    26641 caccagaacc tgtaccactg ccattgccag gattgttccc agaattacgg ccggagaggc
    26701 agctcctgtc atcaccatcc agaacggaat caccgtcaga atgatcgaca ggataaagcc
    26761 tgtcatgtag gtttttacgc tgccatggga cgcgccgctg tgatcggtag aatgactcat
    26821 tacatcgccc ccatcagata aacaacagtg aatacacaga tccaaaccac atccaggaag
    26881 tgccagaaca ggctcaggca catgatgcgg gtacgattag tgctggtcag gccgcgacgg
    26941 gcgatttgca ccatcagcac cgccatccag ataagaccgg aagtgacgtg cagaccgtgc
    27001 gtgccgacca gcgcaaagaa cgctgacagg aagccgctgc gatccggacc catgccgtta
    27061 acaatcagat gatggaattc atagagctcc atcccgataa atccggcgcc aaacaaccag
    27121 gtcaacgcca gccaggagac aacctggctt ttgttgtttt tgtacatggc gatagccgcc
    27181 atgccgtagg tgatggagct gaacaacagc aggaaagttt caaccagaac gaacggcagt
    27241 tcgaaaatgt ccttacctgt cgggccgcct gcggtgccgt tcaccagaac ggcataggta
    27301 gcaaacagga tagagaacag aatgcagtcg ctcatcaggt agatccagaa tccgaagact
    27361 ttggttccgc ctgcatcgtg gtgcccgtgt tcgtgcgcgt gggcagtcgc gtgcgtcaaa
    27421 gtatcagttg ccatttttca gccctgcctt agtaatctca tcgaaatgct ggttttccag
    27481 tttttcgatt tctgccaccg gcacgtagta atccacgtcc tcgtcgaagc ttttcacgat
    27541 ccaggtgatg atcatgcctg cgaagccaac aatcgccagc caccagatat gccagatcat
    27601 ggcgaaaccg aagatggtgg agaaagccgc aatgacgata cctgcaccgc tgtttttcgg
    27661 catatgaatt tcttcatagt ggtcaggctt tttgtacgct tcgcctttct ctttcatttc
    27721 ccagaatgca tcacgctcgt gaacgtgcgg cacaacggca aagttataga acggaggcgg
    27781 ggaagaggtt gcccactcca gcgtacggcc accccacggg tcgccagtca ggtcacggtt
    27841 ctggtcgcgg tcacgaatag aaacgtacat ctgaataacg aggcagagaa tacccagcgc
    27901 aatcagtact gcaccgctgg ctgcaatcat cagcatggtg tggaactgcg ggtcaatctg
    27961 ctggctcaaa cgacgggtca tgcccatgaa gcccagcgca tacagcggca taaaggcaac
    28021 gaagaagccg atgatccaga accagaacgc gcgtttaccc caggtttcgt tcagtttgaa
    28081 accgaacgct ttaggccacc agtaggtcat ccctgcgaag cagccgaaga ccacgccgcc
    28141 gatgatcacg ttatggaagt gcgcaatcag gaacaggctg ttatgcagaa cgaagtccgc
    28201 gcccggtact gccagcagca cgccagtcat accgcctacc gagaaggtga cgataaaacc
    28261 gatggtccac agcatcgcag aatggaacac gatgcggccc tgatacatgg tgaacagcca
    28321 gttgaagatc ttcaccccgg tcgggatggc gataatcata gtggtgatac caaagaaggc
    28381 gtttacgttc gcgcccgcac ccatcgtaaa gaagtggtgc agccagacga tgaacgacag
    28441 cacggtgata cagacggttg cccataccag cgaggtataa ccaaacagac gtttacgcga
    28501 gaaggttgcc gcaatttcgg agaacacacc gaaaacaggc aggatcagga tgtaaacttc
    28561 cgggtggccc caggcccaaa tcaggttgat gtacatcatc atgttgccac ccatatcgtt
    28621 ggtaaagaaa tgggtgccca gatagcgatc cagggtcaac aacgcaacgg taaccgtcag
    28681 aattgggaag gaagcaataa tcagtacgtt cgcgcacagt gatgcccagg taaatactgg
    28741 catcttgaac atggtcatgc ccggtgcgcg catcttcaga atggtaacga agaagttgat
    28801 accggtaagc gttgtaccta taccggatag ctggagactc catatccagt aatcgacacc
    28861 gactcccgga ctgtactcta ttcccgatag cggtggatag gccagccagc cggtctgcgc
    28921 aaattcgccc acgccgagag aaacgttaac cagaatcaca ccaacaacgg taaaccagaa
    28981 gcttaggttg ttgaggaacg ggaacgcaac gtcacgcgcg ccgatctgca gcggaaccac
    29041 caggttcatc agaccgataa cgaaaggcat cgccacgaag aagatcataa tcacgccgtg
    29101 cgcggtgaag atctgatcgt agtggtgagg tggcaggaag cccgcttcgc ccgccgaggc
    29161 aagagcctgc tggctacgca tcataatggc gtcagcaaaa ccacgcagca acatcacaat
    29221 cgccacgatg atatacatga taccgaggcg tttatggtcg acggaggtca gccactcttt
    29281 ccacaggtag gtccacttac cgaagtaagt gatcaggcca acgagcgcca gacctcccaa
    29341 aataatgcca gcgatcgtaa ccatgacgat aggttcatgg aacgggactg catcaagtga
    29401 taattttccg aacatcttta ttcttcctca acccctttaa tgggcggatt ccgcgtggct
    29461 catgtccatg ccttccatac cttcatgtgc gctgtgctca ccttctggct gggtcatgtc
    29521 catgctctta ccgtgagcca taaacttgtt aatcacatcg gcaaacaagt ctggtttcac
    29581 gttggagaaa tattccacct ggttgtattc gctaggcgcg gccagttttt cgaacgctgc
    29641 catgtcagac atgctgttcg gcgactgttt cgcttttgcg acccattggt cgaatgcggc
    29701 gcgatccggt gttgcaatag ctttgaactt catgcctgag aagcccgggc cgctgtagct
    29761 ggcggagata ccgtcataag taccgggttc gttggcgatc agatgcaggc gagtctgcat
    29821 accggccatg gcataaatct ggctacccag acgcggaatg aagaaggagt tcatcacgga
    29881 gttggaggtc actttgaagt acaccggagt gttcgccggg aaagcgattt cattcacggt
    29941 tgcaatgccc tgttccgggt agatgaagaa ccatttccag tccatggaaa ccacttcgat
    30001 ggtaatgggc ttctcgtcgt gtgccagcgg cttgctaggc tcaagagcgt gagtggtttt
    30061 ccaggtcagt accgcaagga agatgatgat taagataggt accgtccaga ccacagcttc
    30121 cactttattg gagtgtgacc agttcgggct gtacttagca tctttattgc tcgcacggta
    30181 cttccaggcg aaaccaacag ccatcaagat tgcgggaata acgacaatca acatcaggcc
    30241 aaatgccgtc agtatcagtg aacgttgctc cagaccaatc tgtcctttgg gatctaacag
    30301 cgcagaatta cagccactga gcaatacagt gcctgcaaat aatgacaacc atcccaaact
    30361 tttattgtat ttcctgagtc tcatttaacg acctcaattc cacgggatct ggtggcgttt
    30421 aaagtgtgtg ggcattttac gggaaggtta cattactgta aacatgatta aattcctgtt
    30481 acttggattt ggcttacagg tcacatatgt tgcaaaacat atcaggttga ttttaagaag
    30541 ctgttgcaaa agggcgttat aacaaaaggg gagtgcagaa ggagtctggg cgggcaattg
    30601 gtataaccaa tgcgaaataa aacaattatt taacaaataa ttatcattaa ggtgacaact
    30661 gggtagtggg aaaataaaga ctcattagct gaatcgtgta agaaaaatag aattcataaa
    30721 tggcgtcaat aatttccaga cgctaagccg cacaaaagaa caaatattaa taagcgatgc
    30781 tgatgtttat ttagccgtaa taattacggc gagtgattac tacggctaaa taatatttac
    30841 agattacgtc agatgcgttt ttcgtagcgc cagataatcc agtaaaccac caagcacgac
    30901 gccagaaagg gcgactaaaa ccccgacttc cagcagagca ggcaggaaag agaagtgcgt
    30961 caaatccagc gcgtccatcg tcaacagcag taaccacacg gccaacaggc tgacgcccgc
    31021 cgccagtgtc cacatggcaa aggcataacc tgccggatat tcggtacggg agataaagtt
    31081 atcatttact cgtgtatatt caagcgtctg gcgacaaacc agcagcaaga taagccctgg
    31141 tacggcagcg gcgacggaga atagatagaa tgtcgaccag ccgtgtgctt caacaaacca
    31201 acccgccacg gggccgacat aaactcgccc tacagcagaa agcgctgaga gcagggcaaa
    31261 ttgagtagcg gaaaatgact tattacacag cgtcattaac agcgcgacaa aggctgatgt
    31321 acccatcccg ccacagaggt tttcgaaaaa gacggctgcg cccatgctgt agagatgctt
    31381 atcagtaatc gacagcagcc agtaaccggc gttagatgca ccttgtaaaa tgccgaaaat
    31441 cagcagtgca cggaacagcg acaggcgctg catcaaaatc ccaccgtaca atgcgccaac
    31501 aatggtcgcc agtaagccaa gcgttttgtt aaccacgcct acttcacccg catcaaaccc
    31561 gacgccgcga atcaaaaacg tggttgtcag gctcatagcg aatgcgtcgc ccagcttata
    31621 cagcacgata agaagcaaaa taagccaggc attattgcga ccaaagaaat ctcgcagagg
    31681 tgcaacaacc gcttgttcca gcgttttggg cacaggaatg gtgtcggtgg gttctggtgc
    31741 aagcaacgtc gcgataatac aggggatcaa cagcgccgcc atcaaccagt acatgccctg
    31801 ccagcccagc catttatctg ccagccacag ggctaagccg ccggaaacca gcatccctaa
    31861 acggtaaccc agcacgctga ttgccgcgcc cgcaccacgt tcttctgccg ggagcacatc
    31921 ggttttccac gcatcgaaga caatatcctg ggaggcagag caaaaagcga tcaccaccgc
    31981 cagtgccgcc atccagcgga gctgggtgcc tggttcgaga aaacccatcg ccgcaatggc
    32041 gactaataac agaatttgcg tggcgagcag ccaaccgcgc cgccgcccga aaaatggggg
    32101 cgtgtagcgg tccatcagtg gtgaccagag gaatttaaaa acgtaagcct ggcctaccag
    32161 agagaagaaa ccaatggttt tgagatcgat attctcgacc gtcatccagg cctgtaaggt
    32221 gccggacgtc agggcgagcg gtagcccgga agcaaaaccc aggatcagca atatggctga
    32281 acgcggctgt tgaaaaatac gtaaatattg actggacatg ggcgtctaca ggcccggctt
    32341 gcaccgggcc agagggcaga aattaacgcg cgttctgctt gatgaattcg tggatgctgg
    32401 tgtcctgaga catatcagcg atggtatcgg tcagcacact gttaaccgca tcggcgatat
    32461 ttttgttgga ggcctggaac gcaccttcaa cgttgtagct ggcacgatag tttttggtca
    32521 ttttgttgcc attctgcgcg gtagcgatga tggcgatatc cgctttggtc gcgatgttgt
    32581 agcgcacgtt gccctgggac acgtcagcat acagttggct aacgatgatt tgcagattaa
    32641 ccgggccatt cggaccaacc atgtatccac gggcggtcat ttgtttttcc agcacttctt
    32701 gcagcaggaa acgcagatcg cgggaggcgg tcagggtaac gatttgatta tcgcgggtga
    32761 cttttgccag cgcctgatcg gtacgctgat cggcaccatt aatgcttacg gtgacgccca
    32821 tcaggcttgg atcctgctgt ggcagtgtaa tcgtcgggga aacttcaata gttgttggcg
    32881 gttttgcgca tcctgcaagc ataaacagag caactaacgg gaagaggatt tttttgaaca
    32941 tgttcgggct ctcagagact cttaagcgtg tttggtaaaa attcccgcca tcataacatt
    33001 gccaacggcg aggggaagtg ggtaaggcat gtaaattcat catgttgacg aaataatcgc
    33061 ccctggtaaa agaaacgctg atgcgaggcc tgtgtttcaa tctttaaatc agtaaacttc
    33121 atacgcttga cagaaaaacc aggacgaaac ctaaatattt gttgttaagc tgcaatggaa
    33181 acggtaaaag cggctagtat ttaaagggat ggatgacatc tcagcgttgt cggaggagat
    33241 atttcatgat gatacgtgag cggatagaag aaaaattaag ggcggcgttc caacccgtat
    33301 tcctcgaagt agtggatgaa agctatcgtc acaatgtccc agccggctct gaaagccatt
    33361 ttaaagttgt gctggtcagc gatcgtttta cgggtgaacg ttttctgaat cgtcatcgaa
    33421 tgatttacag tactttagcg gaggaactct ctactaccgt tcatgcgctg gctctgcata
    33481 cttacactat taaggagtgg gaagggttgc aggacaccgt ctttgcctct cctccctgtc
    33541 gtggagcagg aagcatcgcg taaaaacgca tttgcaactg tcggcgcttt tccagtatgt
    33601 tgctaaagat tttatgaaaa acggcctgcg ggccgttttg tttttgtctg gattttgcgc
    33661 tttttgccca gcattcagac gaaaattgcc cgggaattgt gaaaaaatac gcgacagcgc
    33721 gcaataaccg ttctcgactc ataaaagtga tgccgctata atgccgcgtc ctatttgaat
    33781 gctttcggga tgattctggt aacagggaat gtgattgatt ataagaacat cccggttccg
    33841 cgaagccaac aacctgtgct tgcggggtaa gagttgaccg agcactgtga ttttttgagg
    33901 taacaagatg caagtttcag ttgaaaccac tcaaggcctt ggccgccgtg taacgattac
    33961 tatcgctgct gacagcatcg agaccgctgt taaaagcgag ctggtcaacg ttgcgaaaaa
    34021 agtacgtatt gacggcttcc gcaaaggcaa agtgccaatg aatatcgttg ctcagcgtta
    34081 tggtgcgtct gtacgccagg acgttctggg tgacctgatg agccgtaact tcattgacgc
    34141 catcattaaa gaaaaaatca atccggctgg cgcaccgact tatgttccgg gcgaatacaa
    34201 gctgggtgaa gacttcacct actctgtaga gtttgaagtt tatccggaag ttgaactgca
    34261 gggtctggaa gcgatcgaag ttgaaaaacc gatcgttgaa gtgaccgacg ctgacgttga
    34321 cggcatgctg gatactctgc gtaaacagca ggcgacctgg aaagaaaaag acggcgctgt
    34381 tgaagcagaa gaccgcgtaa ccatcgactt caccggttct gtagacggcg aagagttcga
    34441 aggcggtaaa gcgtctgatt tcgtactggc gatgggccag gggcgtatga ttccgggctt
    34501 tgaagacggt atcaaaggcc acaaagctgg cgaagagttc accatcgacg tgaccttccc
    34561 ggaagaatac cacgcagaaa acctgaaagg taaagcagcg aaattcgcta tcaacctgaa
    34621 gaaagttgaa gagcgtgaac tgccggaact gactgcagaa ttcatcaaac gtttcggcgt
    34681 tgaagatggt tccgtagaag gtctgcgcgc tgaagtgcgt aaaaacatgg agcgcgagct
    34741 gaagagcgcc atccgtaacc gcgttaagtc tcaggcgatc gaaggtctgg taaaagctaa
    34801 cgacatcgac gtaccggctg cgctgatcga cagcgaaatc gacgttctgc gtcgccaggc
    34861 tgcacagcgt ttcggtggca acgaaaaaca agctctggaa ctgccgcgtg aactgtttga
    34921 agaacaggct aaacgtcgcg tagttgttgg cctgctgctg ggcgaagtta tccgcaccaa
    34981 cgagctgaaa gctgacgaag agcgcgtgaa aggcctgatc gaagagatgg cttctgcgta
    35041 cgaagatccg aaagaagtta tcgagttcta cagcaaaaac aaagaactga tggacaacat
    35101 gcgcaatgtt gctctggaag aacaggctgt tgaagctgta ctggcgaaag cgaaagtgac
    35161 tgaaaaagaa accactttca acgagctgat gaaccagcag gcgtaattta cgcagcataa
    35221 cgcgctaaat tcgcacaaag gcccgtcacc gccaggtggt gggctttttt ttgtcatgaa
    35281 ttttgcatgg aaccgtgcga aaagcctctt tcggtgttag cgtaacaaca aaagattgtt
    35341 atgcttgaaa tatggtgatg ccgtacccat aacacaggga ctagctgata atccgtccat
    35401 aaggttacaa tcggtacagc aggttttttc aattttatcc aggagacgga aatgtcatac
    35461 agcggcgaac gagataactt tgcaccccat atggcgctgg tgccgatggt cattgaacag
    35521 acctcacgtg gtgagcgctc ttttgatatc tattctcgtc tacttaagga acgcgtcatt
    35581 tttctgactg gccaggttga agaccacatg gctaacctga ttgtggcgca gatgctgttc
    35641 ctggaagcag aaaacccaga aaaagatatc tatctgtaca ttaactcccc aggcggggtg
    35701 atcactgccg ggatgtctat ctatgacacc atgcagttta tcaagcctga tgtcagcacc
    35761 atctgtatgg gccaggcggc ttcgatgggc gctttcttgc tgactgcagg ggcaaaaggt
    35821 aaacgtttct gcctgccgaa ttcgcgcgtg atgattcacc agccgttggg cggctaccag
    35881 ggccaggcga ccgatatcga aattcatgcc cgtgaaattc tgaaagttaa agggcgcatg
    35941 aatgaactta tggcgcttca tacgggtcaa tcattagaac agattgaacg tgataccgag
    36001 cgcgatcgct tcctttccgc ccctgaagcg gtggaatacg gtctggtcga ttcgattctg
    36061 acccatcgta attgatgcca gaggcgcaac tgtgccgcta tacttatcca gggcggcaca
    36121 acgctgtaag cggcttgcgc ctgagaatgg catttgcgtc gtcgtgtgcg gcacaaagaa
    36181 caaagaagag gttttgaccc atgacagata aacgcaaaga tggctcaggc aaattgctgt
    36241 attgctcttt ttgcggcaaa agccagcatg aagtgcgcaa gctgattgcc ggtccatccg
    36301 tgtatatctg cgacgaatgt gttgatttat gtaacgacat cattcgcgaa gagattaaag
    36361 aagttgcacc gcatcgtgaa cgcagtgcgc taccgacgcc gcatgaaatt cgcaaccacc
    36421 tggacgatta cgttatcggc caggaacagg cgaaaaaagt gctggcggtc gcggtataca
    36481 accactacaa acgtctgcgc aacggcgata ccagcaatgg tgtcgagttg ggcaaaagta
    36541 acattctgct gatcggtccg accggttccg gtaaaacgct gctggctgaa acgctggcgc
    36601 gcctgctgga cgtcccgttc accatggccg acgcaaccac gctgaccgaa gccggttatg
    36661 tgggcgaaga cgttgaaaac atcattcaga agctgttgca gaagtgcgat tacgacgtac
    36721 agaaagcgca gcgcgggatt gtctacatcg atgaaatcga caagatttct cgtaagtcag
    36781 acaacccgtc cattacccga gacgtttccg gtgaaggcgt acagcaggca ctgttgaaac
    36841 tgatcgaagg tacggtagct gctgttccac cgcaaggtgg gcgtaaacat ccgcagcagg
    36901 aattcttgca ggttgatacc tctaagatcc tgtttatttg tggcggtgcg tttgccggtc
    36961 tggataaagt gatttcccat cgtgtagaaa ccggctccgg cattggtttt ggcgcgacgg
    37021 taaaagcgaa gtccgacaaa gcaagcgaag gcgagctgct ggcgcaggtt gaaccggaag
    37081 atctgatcaa gtttggtctt atccctgagt ttattggtcg tctgccggtt gtcgcaacgt
    37141 tgaatgaact gagcgaagaa gctctgattc agatcctcaa agagccgaaa aacgccctga
    37201 ccaagcagta tcaggcgctg tttaatctgg aaggcgtgga tctggaattc cgtgacgagg
    37261 cgctggatgc tatcgctaag aaagcgatgg cgcgtaaaac cggtgcccgt ggcctgcgtt
    37321 ccatcgtaga agccgcactg ctcgatacca tgtacgatct gccgtccatg gaagacgtcg
    37381 aaaaagtggt tatcgacgaa tcggtaattg atggtcaaag caagccgttg ctgatttatg
    37441 gcaagccgga agcgcaacag gcatctggtg aataattaac cattcccata caattagtta
    37501 accaaaaagg ggggatttta tctccccttt aatttttcct ctattctcgg cgttgaatgt
    37561 gggggaaaca tccccatata ctgacgtaca tgttaataga tggcgtgaag cacagtcgtg
    37621 tcatctgatt acctggcgga aattaaacta agagagagct ctatgaatcc tgagcgttct
    37681 gaacgcattg aaatccccgt attgccgctg cgcgatgtgg tggtttatcc gcacatggtc
    37741 atccccttat ttgtcgggcg ggaaaaatct atccgttgtc tggaagcggc gatggaccat
    37801 gataaaaaaa ttatgctggt cgcgcagaaa gaagcttcaa cggatgagcc gggtgtaaac
    37861 gatcttttca ccgtcgggac cgtggcctct atattgcaga tgctgaaact gcctgacggc
    37921 accgtcaaag tgctggtcga ggggttacag cgcgcgcgta tttctgcgct ctctgacaat
    37981 ggcgaacact tttctgcgaa ggcggagtat ctggagtcgc cgaccattga tgagcgggaa
    38041 caggaagtgc tggtgcgtac tgcaatcagc cagttcgaag gctacatcaa gctgaacaaa
    38101 aaaatcccac cagaagtgct gacgtcgctg aatagcatcg acgacccggc gcgtctggcg
    38161 gataccattg ctgcacatat gccgctgaaa ctggctgaca aacagtccgt tctggagatg
    38221 tccgacgtta acgaacgtct ggaatatctg atggcaatga tggaatcgga aatcgatctg
    38281 ctgcaggttg agaaacgcat tcgcaaccgc gttaaaaagc agatggagaa atcccagcgt
    38341 gagtactatc tgaacgagca aatgaaagct attcagaaag aactcggtga aatggacgac
    38401 gcgccggacg aaaacgaagc cctgaagcgc aaaattgacg cggcgaaaat gccgaaagag
    38461 gcaaaagaga aagcggaagc agagttgcag aagctgaaaa tgatgtctcc gatgtcggca
    38521 gaagcgaccg tagtgcgtgg ttatatcgac tggatggtac aggtgccgtg gaatgcgcgc
    38581 agcaaggtca aaaaagacct gcgtcaggcg caggaaatcc ttgataccga ccattatggt
    38641 ctggagcgcg tgaaagatcg catccttgag tatcttgcgg ttcaaagccg tgtcaacaaa
    38701 atcaagggac cgatcctctg cctggtaggg ccgccggggg taggtaaaac ctctcttggt
    38761 cagtccattg ccaaagccac cgggcgtaaa tatgtccgta tggcgctggg cggcgtgcgt
    38821 gatgaagcgg aaatccgtgg tcaccgccgt acttacatcg gttctatgcc gggtaaactg
    38881 atccagaaaa tggcgaaagt gggcgtgaaa aacccgctgt tcctgctcga tgagatcgac
    38941 aaaatgtctt ctgacatgcg tggcgatccg gcctccgcac tgcttgaagt gctggatcca
    39001 gagcagaacg tagcgttcag cgaccactac ctggaagtgg attacgacct tagcgacgtg
    39061 atgtttgtcg cgacgtcgaa ctccatgaac attccggcac cgctgctgga tcgtatggaa
    39121 gtgattcgcc tctccggtta taccgaagat gaaaaactga acatcgccaa acgtcacctg
    39181 ctgccgaagc agattgaacg taatgcactg aaaaaaggtg agctgaccgt cgacgatagc
    39241 gccattatcg gcattattcg ttactacacc cgtgaggcgg gcgtgcgtgg tctggagcgt
    39301 gaaatctcca aactgtgtcg caaagcggtt aagcagttac tgctcgataa gtcattaaaa
    39361 catatcgaaa ttaacggcga taacctgcat gactatctcg gtgttcagcg cttcgactat
    39421 ggtcgcgcgg ataacgaaaa ccgtgtcggt caggtaactg gtctggcgtg gacggaagtg
    39481 ggcggtgact tgctgaccat tgaaaccgcg tgcgttccgg gtaaaggcaa actgacctat
    39541 accggatcgc ttggcgaagt gatgcaggag tccattcagg cggcgttaac ggtggttcgt
    39601 gcgcgtgcgg aaaaactggg gatcaaccct gatttttatg aaaaacgcga catccacgtc
    39661 cacgtaccgg aaggtgcgac gccgaaagat ggtccgagtg ccggtattgc tatgtgcacc
    39721 gcgctggttt cttgcctgac cggtaacccg gttcgtgctg atgtggcaat gaccggtgag
    39781 atcactctgc gtggtcaggt actgcctatc ggtggtttga aagaaaaact actggcagcg
    39841 catcgcggcg ggatcaaaac agtgttaatt ccgttcgaaa ataaacgcga tctggaagag
    39901 attcctgaca acgtaattgc cgatctggat attcatcctg tgaaacgcat tgaggaagtt
    39961 ctgactctgg cgctgcaaaa tgaaccgtct ggcatgcagg ttgtgactgc aaaatagtga
    40021 cctcgcgcaa aatgcactaa taaaaacagg gctggcaggc taattcggac ttgccagcct
    40081 ttttttgtct cgctaagtta gatggcggat tgggcttgcc cttattaagg ggtgttgtaa
    40141 ggggatggct ggcctgatat aactgctgcg cgttcgtacc ttgaaggatt caagtgcgat
    40201 ataaattata aagaggaaga gaagagtgaa taaatctcaa ttgatcgaca agattgctgc
    40261 aggggctgat atctctaaag ctgcggctgg ccgtgcgtta gatgctatta ttgcttccgt
    40321 aactgaatct ctgaaagaag gggatgatgt agcactggta ggttttggta cttttgccgt
    40381 taaagagcgt gctgcccgta ctggccgcaa tccgcagacc ggtaaagaga tcaccatcgc
    40441 tgctgctaaa gtaccgagct tccgtgcagg taaagcactg aaagacgcgg taaactaagc
    40501 gttgtcccca gtggggatgt gacgaagttc aagggcgcat ctactgatgt gcctttttta
    40561 tttgtattcg gtgactttct gcgtcttgtg ggctgacaat tgcccccgtt tcttgtcaca
    40621 atatgccttt gcgcgcatcg atacgttgcg tgaggtacac agtcatctac agcggagtgt
    40681 tgttacacca tgatggacag cttacgcacg gctgcaaaca gtctcgtgct caagattatt
    40741 ttcggtatca ttatcgtgtc gttcatattg accggcgtga gtggttacct gattggcgga
    40801 ggcaataact acgccgcaaa agtgaatgac caggaaatca gtcgtgggca attcgagaac
    40861 gccttcaaca gcgagcgtaa tcgcatgcag caacagctgg gcgatcaata ctccgagctg
    40921 gcagcgaacg aaggctatat gaaaaccctg cgtcaacagg tgctgaatcg tctgatcgac
    40981 gaggcgctgc tggatcagta cgctcgtgag ctgaaactgg gtatcagcga tgagcaggtt
    41041 aaacaggcga ttttcgcgac cccagccttc caggttgacg gcaaatttga taacagccgc
    41101 tataacggta tcctcaacca gatggggatg accgccgatc agtacgccca ggcgctgcgt
    41161 aaccagctca ctacccaaca gctgattaac ggcgttgccg gtaccgattt tatgctgaaa
    41221 ggtgaaaccg acgagctggc ggcactggta gttcaacaac gcgtggtgcg tgaagcgact
    41281 atcgatgtta acgcgctggc ggcgaagcag cctgtgaccg aacaggaaat tgccagctac
    41341 tacgaacaaa acaaaaacaa tttcatgacg ccggaacaat tccgcgtgag ttacatcaag
    41401 ctggatgccg caacgatgca gcaaccggtt agcgatgcgg atatccagag ctactacgat
    41461 cagcatcagg atcaattcac ccagccgcag cgtacccgtt acagcatcat ccagaccaaa
    41521 actgaagatg aagcgaaagc ggtacttgat gagctgaata agggcggtga ttttgctgca
    41581 ttagccaaag aaaaatctgc cgatattatc tctgcccgca acggcggtga tatgggttgg
    41641 ttagaagatg ccactatccc ggacgaactg aaaaatgctg gtctgaaaaa aaaaggccaa
    41701 ctgtctggtg ttatcaaatc ttcggtcggt ttcctgattg tgcgtctgga cgacattcag
    41761 ccagcgaaag tgaaatcgtt agacgaagta cgtgacgaca ttgcggcgaa agtgaaacac
    41821 gaaaaagccc tcgatgcgta ctacgcgctg cagcagaaag tgagcgatgc ggcaagcaac
    41881 gacaccgagt ctctggccgg tgcagagcaa gctgccggcg ttaaagccac tcagacgggt
    41941 tggttcagca aagataacct gccggaagag ttgaacttca agccagttgc cgacgctatc
    42001 ttcaacggcg gtctggtcgg tgaaaacggc gcgccgggca tcaactctga catcatcacc
    42061 gtagacggcg accgcgcatt cgtgctgcgc atcagcgagc acaaaccgga agcggtgaaa
    42121 ccgttggcag atgttcagga acaagttaag gcactggttc agcacaacaa agctgaacaa
    42181 caggcgaaag tggatgctga gaaactgctg gttgatttga aagcaggcaa aggtgcggaa
    42241 gctatgcagg ctgccggtct gaaatttggc gagccgaaaa ccttaagtcg ttccggtcgt
    42301 gacccgatta gccaggctgc gtttgcactg ccgctgccag cgaaagacaa accgagctac
    42361 ggtatggcga ccgatatgca aggtaatgta gttctgctgg cgctggatga agtgaagcaa
    42421 ggttcaatgc cggaagatca gaaaaaagcg atggtgcagg gtatcaccca gaacaacgca
    42481 caaatcgtct ttgaagctct gatgagtaac ctgcgtaaag aggcgaaaat caaaattggc
    42541 gatgcgctgg aacagcaata atcctgaagc cgcctcgcaa aaaaatgctt tgcagctgta
    42601 acaagaaaag gtcgctttcg cggccttttc catttctgaa cattgccatt tgtttactgt
    42661 tttcactgcc gttaaggtga ttccactgtt aacaaacaag gagaaaacag tatgaaacac
    42721 ggaattaaag cactgctcat taccctgtcc ctggcctgtg ccggaatgtc tcatagtgct
    42781 ctggcggcag ctcctgcggc aaaaccgaca acggtagaaa ccaaagcgga agctcctgca
    42841 gcacaaagta aagcagcagt tccggcgaaa gccagtgacg aagaaggcac ccgggtcagc
    42901 attaataatg ccagcgcgga agagttagcc cgcgcgatga atggcgttgg cctgaagaaa
    42961 gcgcaggcga ttgtcagtta tcgcgaagag tacggtccgt ttaaaacggt ggaggatcta
    43021 aagcaggtgc cggggatggg caattcgctg gtggagcgta atctggcggt attaaccctg
    43081 taattaattt gcatagtggc aattttttgc cagactgaag aggtcatacc agttatgacc
    43141 tctgtactta taacaacaac gtaaggttct cgcgctatgc aaacacaaat caaagttcgt
    43201 ggatatcatc tcgatgttta ccagcacgtt aataacgccc gctatctgga gtttctcgaa
    43261 gaagcccgct gggatgggtt ggaaaatagc gacagttttc agtggatgac ggcccataac
    43321 atcgctttcg tggtggtgaa tatcaatatt aactatcgtc gcccggcggt attaagcgac
    43381 ttgttaacca ttacgagcca gttacagcaa ttaaacggta aaagcggcat cttaagccag
    43441 gtcattacac tggagccgga agggcaggtg gtagcggatg cgcttattac gtttgtttgt
    43501 attgatctta aaacgcagaa agcattagct ctggaagggg aattgcgcga aaagctcgag
    43561 cagatggtta agtaaacgtt ttgtggtgcc ggatgctcaa gccgcatccg gcgacacccg
    43621 gaataattac ttcaacccgg ttttctgctt catcgctgcc atcaccgtcg gtttatcggc
    43681 cagataatga tgcaaaccgt tggcacgtaa attacacgcc gcacaatgac cgcaaccgtc
    43741 gcctttaatg ccgttataac aggtcagcgt ttcgttacgg actaaatcca gcttgccgta
    43801 ataatctgcc agcgcccagg tttccgcttt atcaatccac atcagcggcg tttcaaaacg
    43861 aatgtctttc gccataccca gactgacggc atggttcagt gctttcacaa actcatcgcg
    43921 gcaatccggg tagccggaga aatccgtttc gcagacgccg gtaatcaccg cttctgcttt
    43981 tacctgatac gcatatattg ccgccagtgt caggaacaaa atattacgcc cagggacaaa
    44041 cgtattcggg atgccatcgg cttcaggttc ataatctggc actggaatgc tgtcacgtgt
    44101 caggctgctg accgccagct cgttgagcag cgttacatca agaaccttat gcgcgcgtgc
    44161 ccccagtttc agcgccagtt cgcgtgccac gtcgatttcc gcgcgatgtc gctgaccgta
    44221 atcgaacgtc acgcaatgga cttcatcata ttgttgtaat gcctgcacca gacaggtggt
    44281 agaatcctgg cctccactga acacaacgac agcacgtttc atagataatc ctgcttgaac
    44341 aataaaagcg ttatggtaac gcctgcgatg aacccggacc agctattcat tgcgacggcg
    44401 cgggaagcca ggcttcggta aattcaaacc agcctcgcgg tgtcagtcgc acaccgttca
    44461 cgccgggagg ggcgctaatg cgatagtgat agttgaacag cggcgtcagc gtcgcatctg
    44521 ccattaactg gctaaaaacc gctttcaggg cattaaatcg gttttcctca tcaggcatta
    44581 cttgcaccgc gtccagtgtc gattgcaaat gtgcatatgc tggagcgtcg aaaacatgtg
    44641 gccatagtgg atcgcaacgc agccattgct ccagggtata ttccggtgct tcgccaatta
    44701 atctgtcgcc catcatgagg tctgcgtgtg ccagtagggt cgtgtcgtcc cagttttttg
    44761 cgttatgaaa aataattgtg agttcacagc cttccgctgc cagtgtcgcc tgtagacgtt
    44821 ctgccatggt atgaagttct atcggtaggt gataaaccag cgtcaaggtt ttcggtagtt
    44881 tgacctcatc cggtacttgc cattgcggaa tagtccagcc tggcagtaat gcatgactgg
    44941 cggtgatcag gttttctccg acttctaacg tttgtaataa accggattga tgaataatgg
    45001 aaatcacttt tcgcgcctgc cagagggaca gtcggggact tttgcgcaac gttaaatagc
    45061 aaaaacctaa actgatgcca ctactgacct ggctgacccg ttgcagctcc tccggtttgc
    45121 cgatggtgat ttgcacggga tgccgacaac tggttcccaa atctttttcg aaaagcggcg
    45181 gagttatcca gtactcaacc gctttaagca gcggatgacg taaatggtaa taatcatggc
    45241 tttccaggcg caccagctcc gctgtgaatt gtgttaagcg aaaaggaccc gtgccgatca
    45301 gtgggaattg cggatgcgcc agatggctgc aatagctcgc cagtcggtgc gcaagccagt
    45361 aatcggggcg atgtaaaaag aaggtcagac actgcggatg ggtgacttca atacgcttca
    45421 cgctaataaa taattgatcc cgtgctggca gttgtaacag cagcaataat cgctggtgta
    45481 agtgtgaggc ttttactgca tcgccgttat gccagtgtag ggttgaacga agataaaagt
    45541 cccagcgtaa cccgtcagta gaggtttccc agtgatgtgc taaatcgcca atcgggcgct
    45601 gagtattatt atcgaagcgg gtcaggccgg aaaatatctg cccggccaga tgctgctcgg
    45661 cacggccggg caaaaagccc ggttgtagcg gttcgagcgg gcgatagtag ggaatacgca
    45721 acgtgggcgt atcgttttgc cattgtccgc ccataaacgg ctgtaacaga gtgcgcagct
    45781 cacctggggc cagttgcgcc agctccagca catcttgctg ctttccggtt tccagcgcct
    45841 gttccatcat cgcattgcgt agcgattctg gcgtgaccag aaagcgtagt tgcccgcgtt
    45901 ttccgcgtcc tgactgcgcc tgccactcca gccatcccgc ctcctgtgcc tggcgtaaca
    45961 gcgtacgaac atggcgttcg ctgcaaaaac agcgttcggc cagttcgctg acggtgacgg
    46021 tttgcggctt tccggcggaa ggttgccaca gacgttgata ctggttaaga cggttgagca
    46081 gtcgcatata aacccggaac aatattattt aactattcac tattacttcc gtatatatca
    46141 ggtgatactc aatcaccatt aaccgtgtca cagagtggag aaagaaatgg ctcgtctggc
    46201 agcatttgat atggatggca ctttattgat gcctgaccat catttaggtg agaaaaccct
    46261 ctctactttg gcgcgactgc gtgaacgcga cattaccctc acttttgcca ctgggcgtca
    46321 tgcgctggag atgcagcata ttctcggggc gctatcgctg gatgcatatt tgattaccgg
    46381 caacggaacg cgcgtgcatt ctctggaagg tgaactttta catcgtgatg atttacctgc
    46441 ggatgtcgcg gagctggtgc tgtatcagca gtgggatacc cgagccagca tgcatatctt
    46501 caatgacgac ggttggttta ccgggaaaga gatccctgcg ttgttgcagg catttgtcta
    46561 tagtggtttt cgttatcaga taatcgatgt caaaaaaatg ccactcggca gcgtcaccaa
    46621 gatctgcttc tgtggcgatc acgacgatct tacacgcttg cagatccagc tatacgaagc
    46681 attaggcgag cgtgcacatt tgtgtttttc cgccacggat tgcctcgaag tgctgccggt
    46741 gggctgcaat aaaggcgctg cattgacggt gctgacccaa catttaggtt tatcgttgcg
    46801 cgattgcatg gcctttggtg atgcgatgaa cgatcgcgaa atgttaggca acgtcggtag
    46861 cggatttatt atgggcaatg cgatgccgca attgcgcgcg gagctcccgc atttaccggt
    46921 gattggacat tgccgaaatc aggctgtctc tcactatttg acgcactggc tggactatcc
    46981 acatctacct tattcccccg aataacgaga tcccttccag caccgggcaa ttgcccggtt
    47041 ttttttgcgt tgaatttgtc attttgtgcc gtggtgttta aaccgcacag aataaattgt
    47101 cgcgatttca cctttaaaat agaattaaaa gagaaaaaaa ttctctgtgg aagggctatg
    47161 ttagataaaa ttgaccgtaa gctgctggcc ttactacagc aggattgtac cctctctttg
    47221 caggcactgg ctgaagccgt taatctgaca accaccccgt gctggaagcg tctgaaacgg
    47281 ctggaggacg acggtattct tatcggcaaa gtcgccctgc tggatccgga aaaaataggc
    47341 ctcggcctga ccgcctttgt gctgataaaa acgcaacatc acagcagcga atggtattgc
    47401 cgctttgtca cggtggttac cgaaatgcca gaagtgctgg ggttctggcg catggccggt
    47461 gaatacgatt atctgatgcg cgtccaggtt gccgacatga aacgctacga cgagttttat
    47521 aagcgtttgg taaacagcgt gccggggctg tcggacgtca cttccagctt cgcgatggaa
    47581 cagattaaat acaccacttc tttacccatc gaataaatat ccagaatcag gtcaggacac
    47641 aacgcgtgcg attatttgct caattaagct ggtatttccg tcgggaatgg cgtcgctatc
    47701 tcggggctgt cgccttgctt gtcattatcg cgatgctgca actggttccg ccaaaagtgg
    47761 ttggtattgt tgttgatggc gtgacagaac aacactttac taccgggcag atcctgatgt
    47821 ggatcgccac catggtgctg attgccgttg tggtttatct cctgcgttac gtctggcgtg
    47881 tattgctgtt tggcgcgtct tatcaactgg ctgttgaact gcgtgaagat tattaccgtc
    47941 agctaagccg gcagcatcct gagttttacc tgcgtcatcg cacgggtgac ctcatggctc
    48001 gtgcgaccaa tgacgtcgat cgcgtcgtgt ttgccgccgg agaaggggtg ctgacgctgg
    48061 tggattcact ggtaatgggc tgcgctgtgt tgattatgat gtctacgcaa attagctggc
    48121 agttgacctt atttgccctg ttgccgatgc cagtgatggc gatcatgatt aagcgcaacg
    48181 gcgatgcctt gcatgaacgc tttaagctgg cgcaggcggc gttttccagt cttaatgacc
    48241 gcacccagga aagcctcacc agtatccgca tgatcaaagc ttttggtctg gaagatcgcc
    48301 agtcggcgtt atttgccgag gatgccgaag ataccggcaa aaaaaacatg cgggtggcgc
    48361 gtattgatgc tcgtttcgac ccgaccatct atatcgcgat tggtatggcg aacttgctgg
    48421 cgattggcgg cggtagttgg atggtggtgc agggcagttt aacgctgggc cagctcacca
    48481 gttttatgat gtatttaggt ctgatgattt ggccaatgct ggcgctggca tggatgttta
    48541 acatagtgga acgtggtagt gctgcgtaca gccgcattcg cgcgatgctg gcggaagcgc
    48601 cggtggtgaa tgatggtagc gaacccgtgc cggaaggtcg tggcgaactg gatgtaaata
    48661 ttcgccagtt cacgtatccg cagactgacc atcctgcgct ggaaaacgtc aatttcgccc
    48721 tgaaacccgg tcagatgctg ggtatttgcg ggccgactgg ttccggcaaa agtaccctgt
    48781 tgtcgctcat tcagcgtcat ttcgacgtca gcgaggggga tatccgcttt catgatattc
    48841 ctctgacgaa gttacaactc gatagctggc gtagccgcct ggcggtagtt agccagacac
    48901 cgttcctttt ttccgacacc gtagcgaaca acatcgcgtt gggttgcccg aatgccaccc
    48961 agcaagagat tgagcatgtc gcgcggttag ccagcgtgca tgacgatatt ttgcgtttac
    49021 cgcaaggtta cgatacagaa gtgggcgagc gcggcgtgat gctttccggt gggcaaaaac
    49081 agcgtatctc cattgctcgt gcgttattag tcaacgcgga aatcctcatc cttgatgatg
    49141 cgctttcggc agtagacgga cgcacagagc accagatcct gcataacctg cgtcagtggg
    49201 ggcagggaag aacggtaatc atcagcgccc atcgcctttc tgcactgacg gaagccagtg
    49261 aaattattgt gatgcagcac ggacatatcg ctcagcgtgg caatcatgat gagctggcgc
    49321 aacaaagcgg ctggtatcgc gatatgtatc gctatcaaca actggaggcg gcgctcgacg
    49381 acgctccgga aattcgcgag gaggccatcg atgcgtagtt ttagccaact gtggccgact
    49441 ctcaagcgtc tgttagcgta cggttcgccg tggcgtaaac cgctggggat tgcggtcata
    49501 atgatgtggg ttgcggcagc ggcagaagtc agtgggccgc tgcttatcag ctattttatc
    49561 gacaatatgg tagcgaaaaa taacctgccg ttgaaagtgg ttgcagggct ggctgcggcg
    49621 tatgttgggc tgcaactgtt tgccgccggg ctacattacg cgcagtcgct gctgtttaat
    49681 cgggcggcag taggcgtagt gcaacagttg cgtaccgacg tgatggatgc tgcgttacgc
    49741 cagccattaa gcgagtttga tacccaaccc gtcgggcagg tgatttcccg cgtcactaat
    49801 gacactgaag tgatccgcga cctctacgtt accgtagtgg caactgtcct gcgtagcgcc
    49861 gcactggtgg gcgcgatgct ggtggcgatg ttcagcctcg actggcgaat ggcgctggtg
    49921 gcgataatga ttttcccggt ggtgctggtg gtaatggtga tatatcagcg ttacagcacg
    49981 ccgattgtcc gtcgtgtgcg cgcttatctg gcagatatca acgacggctt taacgaaatt
    50041 atcaatggca tgagcgttat ccagcagttc cgtcagcagg cgcgatttgg cgaacgtatg
    50101 ggtgaggcca gtcgttcaca ctatatggcg aggatgcaaa ccctgcgcct cgacggtttt
    50161 ctgctgcgtc cgctgctgag tctgttttca tcgctcattc tttgtggctt gttgatgctg
    50221 tttggcttct ccgccagcgg caccattgaa gtgggcgtgt tgtatgcgtt catcagttat
    50281 ttggggcgac ttaacgaacc cttaatcgaa ctgaccacgc agcaggcgat gctgcaacag
    50341 gctgttgttg ctggtgagcg cgtttttgag ctgatggacg gaccgcgcca gcaatatggc
    50401 aatgatgatc gcccgttgca gagcggcacc atcgacgtcg ataacgtgtc atttgcttat
    50461 cgcgatgaca atctggtgct aaagaacatt aatctctctg tgccttcgcg caactttgtg
    50521 gcgctggtcg ggcataccgg cagtggcaaa agcaccctcg ccagtttatt gatgggctat
    50581 tacccgctaa cggaaggtga gattcgcctt gatggtcgtc cattaagtac gctaagtcac
    50641 agcgcgctgc gccagggcgt ggcaatggtg cagcaagatc cggtggtgct ggcggatacc
    50701 ttcctcgcca acgtgacgct gggacgtgat atctctgaag aacgcgtctg gcaggcgctg
    50761 gaaatcgtgc agctggcgga gctggcgcgt agcatgagtg atggtattta cacgccgctt
    50821 ggcgagcagg ggaataatct ctcagtcggg caaaagcaac tgctggcact ggcgcgcgtg
    50881 ctggtggaga cgccgcaaat cctgatcctt gatgaggcaa ccgccagcat tgactccggg
    50941 actgaacagg cgattcaaca tgctctggcg gcggtgcgtg aacatactac gcttgtggtg
    51001 attgctcacc gcttatcgac tattgttgat gccaacacca ttctggtgct tcatcgtggg
    51061 caagccgtgg agcagggcac tcaccagcaa ctgctggcgg cccagggacg atactggcag
    51121 atgtatcaac tgcaacttgc gggcgaagag ctggcagcca gcgtgcgtga agaggaatca
    51181 ttgagcgcct gaatagcgca atatttcatc gttggtgcaa aaatgtaacg cactgtgcac
    51241 tgtcatagtg cgttttcatt ttcaatcttc ttaacttcct gctctctttc tcgtttttca
    51301 tttttggcac accgcttgca ataccttctt cgtgtagcag aaccattacc gaattctgac
    51361 cggaggggat ctatgaagct ggtgaccgtg attatcaaac cattcaaact tgaagacgtt
    51421 cgtgaagcgt tatcttccat tggtattcag ggcctgaccg tcaccgaagt gaaaggtttc
    51481 gggcgtcaga aaggacatgc cgagctgtac cggggggcgg aatacagcgt caatttcctg
    51541 ccaaaagtaa aaattgatgt ggcgattgct gatgaccaac tcgatgaagt gatcgatatc
    51601 gtcagtaagg cggcttacac cggaaaaatt ggcgacggca aaatcttcgt cgctgaattg
    51661 caacgcgtca ttcgtattcg taccggcgaa gccgacgaag cggcgctgta atctctggca
    51721 cacagcaaca ggaacgaaaa atgaagatag cgacgataaa aactgggctt gcttcactgg
    51781 cgatgctgcc gggactggta atggctgcac ctgcggtggc cgataaagcc gacaatgcgt
    51841 ttatgatgat ttgtactgcg ctggtgctgt ttatgactat tccggggatt gccctgtttt
    51901 acggtgggtt gattcgcggc aaaaacgtgc tgtcgatgct gacgcaggtg acggtgacgt
    51961 ttgcactggt ctgtattctc tgggtggttt acggttactc gctggcgttt ggtgagggaa
    52021 acaacttctt cggcaacatt aacgggttga tgctgaaaaa catcgaactg acggcggtga
    52081 tgggcagcat ttatcagtat atccacgtag cgtttcaggg atcgtttgcc tgcattaccg
    52141 taggcttgat agttggagca ctggcggaac gaatccgctt ctcagctgtg ttgattttcg
    52201 tggtggtatg gctgacgctc tcctacattc cgattgcgca tatggtctgg ggcggcggtt
    52261 tgctggcttc tcacggtgca ctggatttcg cgggtggcac ggtggtgcac attaacgccg
    52321 caatcgccgg tctggtgggc gcgtatctga taggcaaacg cgtgggcttc ggcaaagagg
    52381 cgtttaaacc gcacaacctg ccgatggtct tcaccgggac tgccattctc tatatcggtt
    52441 ggtttggctt taacgccggg tcagcgggca cggcgaatga aatcgcagca ctggcatttg
    52501 tgaatactgt ggtcgcaacg gcggcggcaa ttcttggctg gatcttcggt gaatgggcgc
    52561 tgcgtggtaa gccttcactg ctgggagcgt gttcaggcgc gattgccggt ctggtcggcg
    52621 tgacgccagc gtgtggctac attggtgttg gcggcgcgtt gattatcggc gtggtagctg
    52681 gtctggcggg cttgtggggc gttaccatgc tcaaacgctt gttgcgggtg gatgatccct
    52741 gcgatgtctt cggtgtgcac ggcgtttgtg gcattgtcgg ctgtatcatg accgggattt
    52801 ttgctgccag ctcgctgggc ggcgtgggct tcgctgaagg tgtgacgatg ggccatcagt
    52861 tgctggtaca gctggaaagc atcgccatta cgatcgtctg gtccggtgtg gtggcattta
    52921 ttggctacaa actggcggat ctgacggttg gtctgcgtgt gccggaagaa caagagcgag
    52981 aagggctgga cgtcaacagc cacggcgaga atgcctataa cgcgtaacaa gcactgcaaa
    53041 aaacagccgg acggttttca cctccggcta tttttttaat tatgattacg cattacccct
    53101 tcctgaacgg tcgaggcaac cagcacgccg tcctgagtat aaaactcacc gcgcacaaag
    53161 ccgcgtgcgc tggacgccga ggtgctctcc acgctataca gcagccattc attcaaatta
    53221 aacgggcgat ggaaccacat ggaatggtcg atggtcgcta tctgaattcc cggttcgaga
    53281 aaaccgatgc cgtgcggctg tagagctacc ggcaggaagt taagatcaga agcgtaaccg
    53341 agcagatact gatgaacgcg cagatcatcc ggcacgctac catttgcgcg gatccacacc
    53401 tgacgatgcg gttctgcgac gtgacctttc aatgggttat gaaattccac cggacggact
    53461 tccagcggac gatcgcagat gaatttatct ttcagcactg gcggcagcag gtgcgccagc
    53521 gattgggcga tttgtgtttc cgaaggcagg ccatcaggcg ctggcgcgga cggcattgtt
    53581 ttttgatgtt cgaaacccgc ttctggtgcc tggaaagagg ccgtcatata aaaaatcggt
    53641 ttgccgtttt gaatagcagc aacccggcgg gcgctgaagc tgttaccgtc acgcagcgtt
    53701 tcgacatcat aaataatcgg cttcttacta tcgcctgggc gaagaaagta gctgtgaaac
    53761 gaatgtacca gccgctcttc agggacggtc tcttttgcag catacaaggc ctgacccacg
    53821 acctggccgc caaacacctg gcgtaaacct aaatcttcac tctggccgcg aaagagtcct
    53881 tcctcaattt tttccagatt taacaatgtc agtaaatttt ttagcgcctg actcatataa
    53941 ctctccagta acaaagctgc cgcagcaagc caaagtgagt tgagtataac gcaaatttgc
    54001 cactggtccg atgggtgcaa tggtctgaat tacgggctaa ttacaggcag aaatgcgtga
    54061 tgtgtgccac acttgttgac gttactattt tgttaaccac tcttccggcg aggaaagtta
    54121 gcccgctggt gcattgataa taaggagaag tgaatgaaac tcgtgcacat ggccagtggt
    54181 ttagcggttg cgattgcgtt ggcggcttgc gcagataaaa gcgcggatat tcagacgcca
    54241 gcaccggctg caaatacgtc tatttcagca acacaacctg ctatccagca accgaatgtc
    54301 tccggtaccg tctggatccg tcagaaagtc gcactgccgc ctgatgctgt gctgaccgtg
    54361 acactttctg acgcgtcgtt agccgatgcg ccgtcaaaag tgctggcgca gaaagcggtg
    54421 cgtaccgaag gtaaacagtc accattcagc tttgttctgc catttaaccc ggcagatgtt
    54481 cagccgaacg cgcgtattct attgagtgcg gcgattaccg tgaatgacaa actggtattt
    54541 attaccgata ccgttcagcc ggtgatcaat cagggcggaa ctaaagccga cctgacattg
    54601 gtgccggtgc agcaaaccgc cgtgccggtt caggccagcg gtggcgcaac ggctaccgta
    54661 ccttcgactt caccaactca ggtgaatccg tcttcggcag ttccagctcc tacgcaatat
    54721 taagcgcagt taaccctctc cgcccggaga gggttagtag ttccagcgat aacgctgcaa
    54781 gtcgatttgc ccgcttcccg ataccatcac accttctgcc agtaatgcct gtcgttgacg
    54841 ctgtaaatcc ggtccggtta gcgaaattgt gccgtggcga ttaaccaccc ggtgccaggg
    54901 caaggtgctg ccttcgggga gacgctttaa cacaccgcct acctggcgcg cggcgcgcgg
    54961 cgatcccgcc agtttcgcca catcaccgta agtggtgaca tagccttcgg gaatagcggc
    55021 gacgatttgc cagacgcgtt ggggaaatga atcttctttt tccatctttt cttcctgagg
    55081 taatttttca gcataatctg gaaaaacgcc cgagtgaagc cgcattgcgc aagaaaccag
    55141 catctggcac gcaatgggtt gcaattagcc ggggcagcag tgataatgcg cctgcgcgtt
    55201 ggttctcaac gctctcaatg ggggctctgt tggttctccc gcaacgctac tctgtttacc
    55261 aggtcaggtc cggaaggaag cagccaaggc agatgacgcg tgtgccggga tgtagctggc
    55321 agggccccca cccatttctg cctccccccg tttcgtcaaa aaatcccaac atggctaaac
    55381 tttaaccaca actgacgtcg caagaattgt ctggctgcgc agtacgcttc ggaggtatgt
    55441 ctgatgaagt atgttgatgg ttttgttgtt gccgttcctg ccgataaaaa ggatgcctat
    55501 cgggaaatgg ccgctaaggc cgcgccatta tttaaagagt tcggtgcgct tcgtattgtc
    55561 gaatgctggg ccagcgatgt accggatggt aaagtgaccg attttcgtat ggcggtgaaa
    55621 gcggaagaga atgaagaggt tgtctttagc tggattgaat acccttcaaa agaggtccgc
    55681 gacgctgcta atcaaaagat gatgtcggac ccacggatga aagagttcgg cgagtccatg
    55741 ccgtttgacg gcaagcgaat gatctatggt ggattcgaat caatcatcga cgagtaggta
    55801 gcgtgacggg ctgtgcggcg tgccgtcagc cctaaaattc acaaatgttg ctcggcccag
    55861 cgtaagaaat cctctttcgg taatgccttg ctgtagagcc agccctgacc gtagtgcacg
    55921 ccatgctggc gtaaccactc ttcttgttta ctggtttcga ttccctccgc taccattttc
    55981 agtttcagtg tttttgccat ttcgatgata tgcggcgtga catttttata ttccagcgca
    56041 tcaacgaaag atttatcgat cttcagaatg tcgacatcca gatcctgtaa ataacttaaa
    56101 cttgaatacc ccgtaccaaa atcatcgaga taaatttcat ggcccgcctc ccggtagcga
    56161 gaaattatcg gggcgctggt tttcggatcg gcaaactcgc gttcagtgag ttcaagcgcg
    56221 atctgtctgg gattaacctg atagtgattg atcatttcac gcagcaattg cgggattttt
    56281 tccgaggtga gcacggtgga ttcaagattg atcgaaatat gctgctgcgg atgctgacgc
    56341 agccagtcgc ccatatcttc gaagaggctt cttatgatca gtagcgtcaa cggctcagaa
    56401 aggcccgttt gctgtgccag cggaataaaa ctatctggtg acaaccaact accgtctgtc
    56461 tgcggccagc gcgccagtgc ctcagcaccg acaattttgc cattggctaa ggagacaatc
    56521 ggctgatagt gcacgcaaat atcacgattt tcgatagcat cctgcagccg atgatgcggt
    56581 gactggatac ggcgcaaaat acgcagcaca aacatcgctg ccagcaggcc aatcaccaac
    56641 ccgagcggta accaaataaa gacttgccga tgccagcctt tctgcaacat tttcgttgaa
    56701 gaccaggtga tgatcgaaat attcatttcc gggaagggca ggatatcgta aatgattcca
    56761 ttattttcga tatgctcacc gggtgttttt tgtagtctgg taataattcc ctgagcaatt
    56821 tcatcgctgc tggttatcac aacgttatgg gcattgccaa taatggcggc atcaatttgc
    56881 catgagctat agggaatgac atcaataaag gaagcggggt cgatcatgac gacataatgt
    56941 gccgttccca tggcaaccat gtaacggata atgcctaaat cgttatgcga tgttaaccag
    57001 acacgataac catctttcga aattttacct ggctcgggga aggtatcggg cgggctttca
    57061 tgctccagag acgaacactg gggaacgttg ttatcgatat aaaccacttc ctgaatatag
    57121 cgataactgt aagatacccg acgcatttcc atgagatggg cttcgctaca ggcagcgcct
    57181 tgccatcttt ccagctcttg cagcgcatct ttcccttgtg tcgccacctt attggcccga
    57241 atagcgacgc gggaggaata cgtattcagc tcttcaataa atgatgtttc tacctgctga
    57301 tgggccagcc agatgcttaa gccgacaggc agcaatactg aaagaatcag tactcccgaa
    57361 ataaggccga ccagatgtcg tgttctcacc agcatatcct tattaacaca gtgtagggaa
    57421 agtatatctg attgtggaaa aaggctggca ggagataagg aggtagagcc gggatttcat
    57481 gaagggatgg gtggaaacga acgttgtcat ttgaccaggc gcagagcttt acgcctggta
    57541 tgtaacccta acttaagatg ttgattcggc gcgagcgagg gtaaaaatat cgtaaaaaga
    57601 tagttcacct ttacggacga tcattttttg cagttgttct ttatgaacat ccgcgacacg
    57661 cggagagttc aggatggctt caactagcgc ggcaggtaca aagcgcgtgt tgtaccattc
    57721 gccgttatag caaacgcgga aatccagcac gtcgatatct tcatagcggt agcgtgggta
    57781 gacatcgaaa aagaagaaac cattaagcag gataaataat acaaccagta gccagacaga
    57841 gcctgaaagc gtttcggact gcaacatcac tgccagggtg gcaaaaaaag caacgtacat
    57901 accgataaac aaccccggat gtttacggat aaaactgata ctaaagcggg gtttgttgtc
    57961 gcgtttttcg cgggtattta gagactcaat tgtttcggtc agcaggcgtt gtatttcggt
    58021 catcgtttgc cttcgtggta tctgcaactt tccagggaac accctatttt aacgtgggaa
    58081 tagtagaggg aaagtaacag atttaccaca attttgcata acagttgcga aaaaccgatt
    58141 acaatttttt aattattctg gctggattac cgcccacgac aacgttgtcc gggacatctt
    58201 ttgtgacaac tgcacctgag gctaccacga cgttatcacc aatggtcaca ccagggttaa
    58261 tgaccgcacg tccgccaatc cagacgttat taccgatggt gacgggtttc cccagttcag
    58321 caccgctatt acgtgctaca gggtcgatgg gatgtgttgc cgtgtagata tgaacgcctg
    58381 gtgccaacat acagttatca ccaatgcgaa tagggcagac atcaagcatc acgcaatcga
    58441 agttggcgaa aaaattattg ccgagaaaaa tgttataacc atagtcacag cgaaacgttg
    58501 gctcaatata agcctctgtc acctgaccga ataaatcagc aagaatttgc tggcgtaatg
    58561 tgtgctcttc cgccagggaa tgattgtatc ggtgaataag ccgacgagcg cgcaggcgat
    58621 cgcgagataa cgcctcatct gccgagtgat acaactcacc agcaatcatc ttttcttttt
    58681 ctgtgctcat tactgacctc cggttgctga aaatgcggca acacagtaag acgaaaagtc
    58741 agtgaagaga acgttcccat tacatctgtg tgatgaagat cacatagggt atgccttcat
    58801 ggcgaaagaa ttataaaact cttaataaac agccggttat agctccgaaa gcgagttatt
    58861 tattagcgaa taaatttcca tactgaggaa gggatcttgt cgtacagttt attcatggtc
    58921 aattcggcga ggcggtgatc tgcggctgag taaaataccg ccagctcatt atctgataat
    58981 tcgtatttat ttttctcgat aacacgctcc agcgtgtcaa ttgtctggca acgacgtaaa
    59041 cgcattaaat aatcggtttt
//
LOCUS       DAZKGF010000008        59046 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZKGF010000008 DAZKGF010000000
VERSION     DAZKGF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.008"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..207)
                     /locus_tag="ACHOQV_000503"
     CDS             complement(<1..207)
                     /locus_tag="ACHOQV_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFP9628928.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLK"
     gene            complement(384..1295)
                     /gene="gsiD"
                     /locus_tag="ACHOQV_000504"
     CDS             complement(384..1295)
                     /gene="gsiD"
                     /locus_tag="ACHOQV_000504"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFP9628929.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGASIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1298..2218)
                     /gene="gsiC"
                     /locus_tag="ACHOQV_000505"
     CDS             complement(1298..2218)
                     /gene="gsiC"
                     /locus_tag="ACHOQV_000505"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFP9628930.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVGDEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2236..3774)
                     /gene="gsiB"
                     /locus_tag="ACHOQV_000506"
     CDS             complement(2236..3774)
                     /gene="gsiB"
                     /locus_tag="ACHOQV_000506"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFP9628931.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3794..5665)
                     /gene="gsiA"
                     /locus_tag="ACHOQV_000507"
     CDS             complement(3794..5665)
                     /gene="gsiA"
                     /locus_tag="ACHOQV_000507"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFP9628932.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     SGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYRGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVVAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5652..6617)
                     /gene="iaaA"
                     /locus_tag="ACHOQV_000508"
     CDS             complement(5652..6617)
                     /gene="iaaA"
                     /locus_tag="ACHOQV_000508"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9628933.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     AMVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6821..8056
                     /gene="moeA"
                     /locus_tag="ACHOQV_000509"
     CDS             6821..8056
                     /gene="moeA"
                     /locus_tag="ACHOQV_000509"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFP9628934.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            8056..8805
                     /gene="moeB"
                     /locus_tag="ACHOQV_000510"
     CDS             8056..8805
                     /gene="moeB"
                     /locus_tag="ACHOQV_000510"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFP9628935.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYHCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCGVCGQ"
     gene            complement(8881..9543)
                     /gene="fsa"
                     /locus_tag="ACHOQV_000511"
     CDS             complement(8881..9543)
                     /gene="fsa"
                     /locus_tag="ACHOQV_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9628936.1"
                     /translation="MELYLDTSDVVAVKTLSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            9674..10573
                     /gene="ybiY"
                     /locus_tag="ACHOQV_000512"
     CDS             9674..10573
                     /gene="ybiY"
                     /locus_tag="ACHOQV_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFP9628937.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10579..13011
                     /gene="ybiW"
                     /locus_tag="ACHOQV_000513"
     CDS             10579..13011
                     /gene="ybiW"
                     /locus_tag="ACHOQV_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFP9628938.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQAKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPIGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13157..13972
                     /gene="ybiV"
                     /locus_tag="ACHOQV_000514"
     CDS             13157..13972
                     /gene="ybiV"
                     /locus_tag="ACHOQV_000514"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFP9628939.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNIAPFNS"
     gene            14124..15383
                     /locus_tag="ACHOQV_000515"
     CDS             14124..15383
                     /locus_tag="ACHOQV_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFP9628940.1"
                     /translation="MTFSCETLPTDAKAAIREMKAALREQLSDVQQVFDTLSAKIETR
                     VAEIDALKAQGLPVWPEVSYSDIAAGTVSDATRNEIKRRGCAVIKGHFPREQAMAWDR
                     AMLDYLDINHFDDVYKGPGDSFFGSLEASRPEIYPIYWSQAQMQARQSENMAAVQSFL
                     NRLWKSESNGKQWFNPDISVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQRV
                     FANVFNGNFDDYDPWDAAHRTDVEEYTVDNTTKCSVFRTFQGWTALSDMLSGQGLLHV
                     VPIPEAMAYVLLRPLLDDVPDDELCGVAPGKVLPISEKWHPLLLKALTSIPAITAGDS
                     VWWHCDVIHSVAPVDNQQGWGNVMYIPAAPMCEKNRAYAHKVKMALEKGASPGDFPRE
                     DYEANWEGRFTLEDLNIHGKRALGMDV"
     gene            complement(15624..17216)
                     /gene="ybiT"
                     /locus_tag="ACHOQV_000516"
     CDS             complement(15624..17216)
                     /gene="ybiT"
                     /locus_tag="ACHOQV_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFP9628941.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIIGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17435..18355
                     /gene="ldtB"
                     /locus_tag="ACHOQV_000517"
     CDS             17435..18355
                     /gene="ldtB"
                     /locus_tag="ACHOQV_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9628942.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18413..19531)
                     /gene="ybiR"
                     /locus_tag="ACHOQV_000518"
     CDS             complement(18413..19531)
                     /gene="ybiR"
                     /locus_tag="ACHOQV_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFP9628943.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGAQTPEWKPRLVWSCLGLYIVFLMALE
                     LKQELWGLVIVAVGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(19528..19995)
                     /gene="mntR"
                     /locus_tag="ACHOQV_000519"
     CDS             complement(19528..19995)
                     /gene="mntR"
                     /locus_tag="ACHOQV_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFP9628944.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20181..20309
                     /gene="mntS"
                     /locus_tag="ACHOQV_000520"
     CDS             20181..20309
                     /gene="mntS"
                     /locus_tag="ACHOQV_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFP9628945.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20581..22164
                     /gene="opgE"
                     /locus_tag="ACHOQV_000521"
     CDS             20581..22164
                     /gene="opgE"
                     /locus_tag="ACHOQV_000521"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP9628946.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22213..22728)
                     /gene="ompX"
                     /locus_tag="ACHOQV_000522"
     CDS             complement(22213..22728)
                     /gene="ompX"
                     /locus_tag="ACHOQV_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFP9628947.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(22781..22846)
                     /locus_tag="ACHOQV_000523"
     CDS             complement(22781..22846)
                     /locus_tag="ACHOQV_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9628948.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23081..23968
                     /gene="rhtA"
                     /locus_tag="ACHOQV_000524"
     CDS             23081..23968
                     /gene="rhtA"
                     /locus_tag="ACHOQV_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFP9628949.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24268..24771
                     /gene="dps"
                     /locus_tag="ACHOQV_000525"
                     /gene_synonym="pexB"
     CDS             24268..24771
                     /gene="dps"
                     /locus_tag="ACHOQV_000525"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFP9628950.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25175..25921
                     /gene="glnH"
                     /locus_tag="ACHOQV_000526"
     CDS             25175..25921
                     /gene="glnH"
                     /locus_tag="ACHOQV_000526"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFP9628951.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26060..26719
                     /gene="glnP"
                     /locus_tag="ACHOQV_000527"
     CDS             26060..26719
                     /gene="glnP"
                     /locus_tag="ACHOQV_000527"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFP9628952.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            26716..27438
                     /gene="glnQ"
                     /locus_tag="ACHOQV_000528"
     CDS             26716..27438
                     /gene="glnQ"
                     /locus_tag="ACHOQV_000528"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFP9628953.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            27555..29780
                     /gene="ybiO"
                     /locus_tag="ACHOQV_000529"
     CDS             27555..29780
                     /gene="ybiO"
                     /locus_tag="ACHOQV_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFP9628954.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTIWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(29777..30703)
                     /gene="rlmF"
                     /locus_tag="ACHOQV_000530"
     CDS             complement(29777..30703)
                     /gene="rlmF"
                     /locus_tag="ACHOQV_000530"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFP9628955.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            30979..31239
                     /gene="mcbA"
                     /locus_tag="ACHOQV_000531"
     CDS             30979..31239
                     /gene="mcbA"
                     /locus_tag="ACHOQV_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFP9628956.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            31504..33786
                     /gene="fiu"
                     /locus_tag="ACHOQV_000532"
     CDS             31504..33786
                     /gene="fiu"
                     /locus_tag="ACHOQV_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFP9628957.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIKNEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            33828..34505
                     /gene="ybiX"
                     /locus_tag="ACHOQV_000533"
     CDS             33828..34505
                     /gene="ybiX"
                     /locus_tag="ACHOQV_000533"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFP9628958.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            34579..34845
                     /gene="ybiI"
                     /locus_tag="ACHOQV_000534"
     CDS             34579..34845
                     /gene="ybiI"
                     /locus_tag="ACHOQV_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFP9628959.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            35110..35370
                     /gene="ybiJ"
                     /locus_tag="ACHOQV_000535"
     CDS             35110..35370
                     /gene="ybiJ"
                     /locus_tag="ACHOQV_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFP9628960.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(35510..36595)
                     /gene="hcxB"
                     /locus_tag="ACHOQV_000536"
     CDS             complement(35510..36595)
                     /gene="hcxB"
                     /locus_tag="ACHOQV_000536"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFP9628961.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAVVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGMPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQKTLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARKVGMPEETLQAFCQQLAS"
     gene            complement(36736..37698)
                     /gene="ybiB"
                     /locus_tag="ACHOQV_000537"
     CDS             complement(36736..37698)
                     /gene="ybiB"
                     /locus_tag="ACHOQV_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFP9628962.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPADKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIKRCLAGSEPIPESLKIQMACCLMATGEAATINDGLARINQAF"
     gene            complement(37726..39876)
                     /gene="dinG"
                     /locus_tag="ACHOQV_000538"
     CDS             complement(37726..39876)
                     /gene="dinG"
                     /locus_tag="ACHOQV_000538"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFP9628963.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            39980..40180
                     /locus_tag="ACHOQV_000539"
                     /pseudo
     CDS             39980..40180
                     /locus_tag="ACHOQV_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
     gene            complement(40415..41776)
                     /gene="rhlE"
                     /locus_tag="ACHOQV_000540"
     CDS             complement(40415..41776)
                     /gene="rhlE"
                     /locus_tag="ACHOQV_000540"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFP9628964.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            42005..42676
                     /gene="cecR"
                     /locus_tag="ACHOQV_000541"
     CDS             42005..42676
                     /gene="cecR"
                     /locus_tag="ACHOQV_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFP9628965.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            42679..43674
                     /gene="hlyD"
                     /locus_tag="ACHOQV_000542"
     CDS             42679..43674
                     /gene="hlyD"
                     /locus_tag="ACHOQV_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFP9628966.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            43667..45403
                     /gene="ybhF"
                     /locus_tag="ACHOQV_000543"
     CDS             43667..45403
                     /gene="ybhF"
                     /locus_tag="ACHOQV_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9628967.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            45396..46529
                     /gene="ybhS"
                     /locus_tag="ACHOQV_000544"
     CDS             45396..46529
                     /gene="ybhS"
                     /locus_tag="ACHOQV_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9628968.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            46540..47646
                     /gene="ybhR"
                     /locus_tag="ACHOQV_000545"
     CDS             46540..47646
                     /gene="ybhR"
                     /locus_tag="ACHOQV_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9628969.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(47608..48018)
                     /gene="ybhQ"
                     /locus_tag="ACHOQV_000546"
     CDS             complement(47608..48018)
                     /gene="ybhQ"
                     /locus_tag="ACHOQV_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFP9628970.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            48151..48912
                     /gene="ybhP"
                     /locus_tag="ACHOQV_000547"
     CDS             48151..48912
                     /gene="ybhP"
                     /locus_tag="ACHOQV_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9628971.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKMQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            48909..50150
                     /gene="clsB"
                     /locus_tag="ACHOQV_000548"
     CDS             48909..50150
                     /gene="clsB"
                     /locus_tag="ACHOQV_000548"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFP9628972.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAMFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            50150..51106
                     /gene="ybhN"
                     /locus_tag="ACHOQV_000549"
     CDS             50150..51106
                     /gene="ybhN"
                     /locus_tag="ACHOQV_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFP9628973.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(51142..51531)
                     /locus_tag="ACHOQV_000550"
     CDS             complement(51142..51531)
                     /locus_tag="ACHOQV_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9628974.1"
                     /translation="MESYSQSSNKLDFQHEARILNGIWLITALGLVTTAGLALVIIRN
                     AALMSERPIWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVSCAFTGALTLY
                     YYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(51737..52441)
                     /gene="ybhL"
                     /locus_tag="ACHOQV_000551"
     CDS             complement(51737..52441)
                     /gene="ybhL"
                     /locus_tag="ACHOQV_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9628975.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(52578..53030)
                     /gene="moaE"
                     /locus_tag="ACHOQV_000552"
     CDS             complement(52578..53030)
                     /gene="moaE"
                     /locus_tag="ACHOQV_000552"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFP9628976.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(53032..53277)
                     /gene="moaD"
                     /locus_tag="ACHOQV_000553"
     CDS             complement(53032..53277)
                     /gene="moaD"
                     /locus_tag="ACHOQV_000553"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFP9628977.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSLDHPLTDGDEVAFFPPVTGG"
     gene            complement(53270..53755)
                     /gene="moaC"
                     /locus_tag="ACHOQV_000554"
     CDS             complement(53270..53755)
                     /gene="moaC"
                     /locus_tag="ACHOQV_000554"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFP9628978.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(53758..54270)
                     /gene="moaB"
                     /locus_tag="ACHOQV_000555"
     CDS             complement(53758..54270)
                     /gene="moaB"
                     /locus_tag="ACHOQV_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFP9628979.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(54292..55281)
                     /gene="moaA"
                     /locus_tag="ACHOQV_000556"
     CDS             complement(54292..55281)
                     /gene="moaA"
                     /locus_tag="ACHOQV_000556"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFP9628980.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(55269..55413)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            55678..56586
                     /gene="yvcK"
                     /locus_tag="ACHOQV_000557"
     CDS             55678..56586
                     /gene="yvcK"
                     /locus_tag="ACHOQV_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP9628981.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(56778..58799)
                     /gene="uvrB"
                     /locus_tag="ACHOQV_000558"
     CDS             complement(56778..58799)
                     /gene="uvrB"
                     /locus_tag="ACHOQV_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9628982.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT        14195 a        15798 c        14970 g        14083 t
ORIGIN      
        1 ctttaatgtc agggccgaac gttcaatatc tttaaagaaa tagccaacgt ttttctgaat
       61 aagttcaaag cggctatcgg cacgttttac cgcgcgttcc gataccagtt gatagttaat
      121 gatgaaaaaa agaccaataa atgacagtgc tgccaataaa aataaaatta tcagccttat
      181 ctttatcttt tcgtataaac tcagcatgtt aatacattta acaaaataac tgtctgaacg
      241 ctgtgccgct gcaaaatagt cccttgctgc ggctttttaa ccaaattacc cgattctaca
      301 aaatcagcag aaagttacaa cacttgctta gggcaataat ttcctgtgag gtgattaccc
      361 tttcaagcaa tattcaaacg taactatcct ttaattttcg gatccagcgc atcgcgtaaa
      421 ccatcgccca acaaattgaa cgccagtacg gtcagaaaaa tagccagggc cggaaaaaca
      481 gcgacatgcg gcgcgataac catatccgct cgtgcctcat tgagcattgc accccactct
      541 ggtgtcggcg gctgcgcacc gaggccgaga aatgacaggc tggcggcaga gataatcgag
      601 gcgccaatgc gcatggtgaa aaacaccacg atagaagaga cggtcccagg caagatatga
      661 cgcaacaaaa tggtcatatc gctggcacca atactgcgtg ctgactcgat aaaggtttgc
      721 tgtttcaaca ccagcgtgtt gccgcgcacc agccgggcaa acgcggggat ggaaaaaatg
      781 gcgactgcga taatcacgtt agcaatgccg cttcccaaca ccgcaacaac agcgatcgcc
      841 agtaaaatac ccgggaaggc aaacaacaca tcgcaaatgc gcatgatcag ccgatcccac
      901 cagccttcat aatatccagc cagcaagccc agcaacgtcc cgatcgccgc accgataaac
      961 acggcaaaca cgcccgccgc cagcgatatt tgcgcaccaa ccaggacacg gctgaaaatg
     1021 tcgcgcccca gtgaatcgac accaaaccag tgctgcaaag aaggtccgtt attcagattg
     1081 tcataatcaa aataattttc ggcgtcatag ggagcgatcc agcgtgcaaa aatggccacc
     1141 acaatcaata aaataacgaa caatgcggcg gtcatcgcca tatgctgacg gcgaaatcgt
     1201 cgccagaatt catgccacgg tgtacgtacc tggtcaggtt tgaccagtgg catggcgttt
     1261 aacaccgcct gacgtcgcca gttaaatagt cgcatcctta cttgtacctg atagccgggt
     1321 taatggcggc gtaaagcaca tccaccacta agttgataag aataaattcc agcgagaaaa
     1381 gcagaatttc cgcctgaatc accgggtaat cgcgcatttc aacggagtca acgagtaagc
     1441 gtccaagtcc gggccagttg aaaactttct ccaccacgat ggaaccaccg agtaaaaatc
     1501 caaactgtaa gcccatcatg gtcactaccg ggatcatcgc gttgcgtagc ccgtgtttga
     1561 ggacaaccca ggtttcgctc acccctttcg ccctcgcggt acgcatataa tcttcgctta
     1621 aaacgtcgac aaacgacgcg cgggtaaagc gcgccatcac ggcggccact gccgcgccga
     1681 gcgtcaggga gggtaaaatg tagtgctgcc aactgtctgc tcccacggta ggcagccagc
     1741 ccagttcaac agagaatacc tgaattaaaa gcatccccag agcaaatgcc ggaaacgaaa
     1801 tccccgacac cgcaatagtc atactcaatc gatccggcca acggttacgc cagacggcgg
     1861 cgataattcc cgccgccata ccaaatatca ccgcccagac catactggtt atggtcagcc
     1921 acagcgttgg cataaagcgg ctggcaatct catcgccaac cggacgacgc gacaccatcg
     1981 acaggccaaa atccccctgc acagcattgc tgatatagtg ccagaactgg tgatacagcg
     2041 gctgatccaa ccccagctgc tgacgcacca gttctataac ctgcgcatca gcttcgggcc
     2101 cggcaattaa tcgcgccgga tcgccgggca gcatgtggac aaataaaaac accagcaccg
     2161 agacgataaa cagcgtcgga atcaacccca gtaagcgttt gataacgtaa ttaagcattc
     2221 cactccctgc gttgcttatt gcaaatccgc atcttcaaag ctgaagccgg tgtctggcat
     2281 gatccaaaaa ccggtcaggt ttttactgtg tgccgacaca agtttttcta ccaccagcgg
     2341 gatccacggc gactcttgcc agatgatatc ctgcgccgcc ttatacaagc gggtcttttc
     2401 cgccggatca ttagttttca gtgcctgagc caggaagtca tccacctgtt tattgctgta
     2461 aaacgcggta ttaaacagcg ttggtggcca gttctgcgag gcaaacagcg gcgatagagc
     2521 ccagtcggct tcgccggttg aagccgacca gccagtgtag aacatccgca cgccgctctc
     2581 tttttgccct ttaccttcaa cttctgccgc ccgctgtccg gcatccatcg cagtcacctg
     2641 ggctttaatc ccaacctgcg ctaactgctg ctgggtaaat tgcagcactt tctgcgcggt
     2701 gctgtggtta tgtgacgacc acagcgtggt actgaaaccg ttgggatatc ccgcctcttt
     2761 cagtaattcg cgcgctttca ctggatcgta aggccacggt ttataacttt gcgcgtaggc
     2821 gatactcggc ggtaccacac cagtagctgg cgttgcatag cccgcgaagg ccactttcac
     2881 caacgccggg cggttaatgg cgtaattcag cgcctcacgg accttcgggt tatcgaacgg
     2941 cttttgcgtc acgttcatac tgatataacg ctgcataatt gacggactgg ccatcaactc
     3001 gatatttttg tttttctcca gcagtgcggc ctgctcgtag ggaatgggga aagcaaactg
     3061 cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta tccgccaccg gacgccaggt
     3121 tatgctgtcc agtttgggca atcctggctg ccagtaaccc gcgaattttt tcaccttcac
     3181 aaaatcggtc tgattccagg tatccagttc atacggcccg gttcccaccg gatgaaaacc
     3241 aatctccttg ccatattttt ccagcgctgc cggtgaaatc atcgcggtcg ccggatgggc
     3301 aagaatatta ataaacgctg agaatggctg tttgagggta atctttaccg ttgtcggatc
     3361 gatcgcttcc gttttggcaa tattcttata caggttatag cgtttaagat gattcgccgg
     3421 atcgctggcc cggtccagat tcgcttttac cgccgctgcg ttgaagtcgg ttccatcctg
     3481 gaatttaatg ccttcccgca gtttcacggt gtaagtaagg ccgtcatcgg aaacggtata
     3541 actctccgcc agcacgtttt tcagtttcat ctctttatcc agaccgaaca gcccctggta
     3601 aaacgatttc gctacggcct gagataacgt gtcattcgcg tcatatggat cgagcgtggt
     3661 gaagttcgat cctaccgcca ccaccacatc tttggcagcg aatgcacaag atgccatcaa
     3721 cgctgtcgca atgcccagcg ccactaaccc actacggtgt acagctcttg ccattttatt
     3781 ctccgcctga atgttatcta cgcatgaatg cgtattctga ttgtggttgt gcgacgtaat
     3841 gccccggccc gacgcattgc aacgagacgg ctacaacctc ttcaccacgc agatgaatat
     3901 tgctgggaag atcgtccgac agcagtacac gctgcggtcg ttgtcgggac ggttcagcga
     3961 ccggaactgc cgccagtaat ttacgcgtat aaggatgctg cgggttttcg aagaccgcgc
     4021 gccgtggacc aatttcaaca atttgtccgc gatacatcac cgccacacga tgactaatcc
     4081 gctctaccac ggccatatcg tgggagataa acagatacgc aatgccgaaa tcacgctgga
     4141 gatcgagcaa caagttgata atctgcccgc gaatagaaac atccagcgcc gagacggctt
     4201 cgtcggcaat gatcactttt ggattcaatg ccaacgcgcg agcaatgcag atgcgctggc
     4261 gctgaccgcc ggaaaactca tgagggtaac gccaggcatg ttccggtaac aggcccacgc
     4321 gctccagcaa ccacgcaacg cgtgcagccg cttctttacc tggcaataaa ccgtgtacgc
     4381 gcagcggttc gataatcgaa tcaccgatgg tctgacgtgg gtccagcgaa gcgtaagggt
     4441 cctgaaaaat aaactgaata tcccggcgca aagcctgaag tttaccggat gacaaggtat
     4501 cgattcgctg accgttaaag ataatttcgc cgccctgcga ttcgaccagg cgcagcaacg
     4561 cccgcccggt agtggattta ccgctgccag actcgcccac cagcgatagc gtttcgccag
     4621 gccagagatc aaaactgact ttctcaacgg catgcacttc ccgcgttacg cgattcaaca
     4681 aaccgctgcg caaagggaaa cgggtgacca gattacgcac ccgtaaaaca ggttcgccat
     4741 ccaccaccgt tttctgctcg atggggggtt cctgttttgc tggatgttca agcgatatca
     4801 atgggaaacg tcggggataa tctaaccctt tcatcgcacc aagttgcgga acagcagcta
     4861 acagcgcacg ggtataagga tgttgcggtg catgaaaaat ctgttcgacg ctacccgttt
     4921 ccaccgcctc gccctgatac atcaccagta cccgatcggc aatctctgcc accacgccca
     4981 tatcgtgagt gataaagata acgcccatcg acatctcttt ttgcaatact ttgattaatt
     5041 gcaggatctg cgcctgaata gtgacatcca acgcggtggt tggctcatcg gcaatcagca
     5101 ccgccgggcg gcatgacagc gccatcgcaa tcatcactcg ctggcgcatc ccgccagaga
     5161 gttgatgcgg ataacgtgaa aggatggttt gtgcctccgg aatgcgtacc tgatccagca
     5221 tccgcttcgc ctcgaccatc gcttcttcac gactggcgtt ctgatgcaga cgaattgatt
     5281 cggcaatctg ttcacccaca gtaaataccg ggttcagcga tgtcatcggt tcctgaaata
     5341 tcatcgccat atccgcaccg cgcacatggc gcatttgtgc agcgctctgc tcgctaagtt
     5401 caatcacttc acgactgcgc cgccgcaaca gcatttgatc gcactgcact aaaccgcccg
     5461 cctgttccaa caagcgcatc aatgccagcg cagtcactga cttaccagag ccggattcgc
     5521 caacaattgc cagcgtctca ccgcgttgca ggctaaaaga gagattgcgg accgcagcta
     5581 ttttctgctg gtcctgcata aaggcaatat tcagattttc aaccgccagc acatcaccgg
     5641 catcaagttc atcactgtgt ggcaacggtg tccccttttt cacggtagat accggtggtt
     5701 ggcgtatcgc ctgcgtagcc ccaggcgcga tacattcctt cggtgttaaa cggtagcgcg
     5761 acgttacctt catggtcgat agcgattaag ccaccgctac cgccaagcgc aggaagtttt
     5821 tccattacta cccgctcgca ggcttccgcg agacttaatc cgccgtaatc cattaacgcg
     5881 gcgatgtcat atgccgccag cgcgcggatg aagacttcgc ccgtgccggt acaagaaacc
     5941 gccacactgg cgttattggc gtagcatccg gcacccacta aggggctatc gccaactcgt
     6001 ccgggtaatt tattggtcat tccgcccgtg gacgtagctg ccgccagatt gccgtctaaa
     6061 tccaacgcca cggcccccac ggtgcccatt ttctgttttt catccagtgg cgcaccgcta
     6121 tggtcgagaa ccattgcccc ttcctcgcgc gctgccagta gttgttcata acgcaaaggc
     6181 gtggagaaaa tctccggcga gacgcgctcc atgccatgag caaacgcaaa attttctgcc
     6241 ccttcgccaa tcatcatcac atgcgggctt tgctccatca ccagccgggc ggcaagaacc
     6301 ggattacgca gatgactaac gcctgccacc gcaccggctt tcagggtgtt accatccatc
     6361 acacaggcgt ccagttcatg ggtttcatca cgcgtaaaga cagcgccaat tccggcgtta
     6421 aacagtggac actcttccag cagacgcacc gcttccgtca ccacatccag cgcactttcg
     6481 cccgcttcca gcattttctg cccggtttca acaatggcag acagcgcctc gatgtagcgt
     6541 aattcctgtt gcagactcat ctgcgcgcgg ctaattgcac ctgcgccgcc atgaattgca
     6601 atgactgctt tgcccatagt attcgtccat taagggtggc tcgccacttt tgctataaat
     6661 atcagaattt ttatgaatta ctcattcaat ttttgaatat agaaagatgg atatggcatg
     6721 taaaggcagg agccaacgct acgcccgagg tggcatacag tctgtggcgt ttttctgaca
     6781 taataggcaa attcgatttt gcctccgcag gagtgttttc atggaattta ccaccggatt
     6841 gatgtcgctc gacaccgcgc ttaatgagat gctttctcgc gttaccccac tgaccgccca
     6901 ggaaacgctg ccactggttc agtgttttgg tcgtattctg gcgagcgatg tcgtttcgcc
     6961 actggatgtc ccggggtttg ataactccgc tatggacggc tacgcggtgc gtttagccga
     7021 tattgcctcc gggcaaccgc tgcccgttgc cggtaaatcc tttgccggtc agccgtacca
     7081 tggtgaatgg cctgcgggta cctgcattcg tattatgacc ggtgcgccgg tgccagaagg
     7141 ctgcgaagcg gtggtgatgc aggagcagac tgaacaaacg gacaatggcg tgcgttttac
     7201 tgctgaagtg cgtagcgggc aaaatattcg ccgtcgcggt gaagatatct ctgcaggtgc
     7261 ggttgttttc ccggcgggga ctcgcctgac taccgctgaa ctgccagtga ttgcttcgct
     7321 agggattgcc gaagttccgg tgattcgtaa agtgcgtgta gcgctttttt ctaccggtga
     7381 tgaactccag ttgcccggtc agccgctggg cgacggccaa atctacgata ccaaccgtct
     7441 cgccgtacac ctgatgttag aacagttggg atgcgaggta atcaacttag ggattatccg
     7501 cgacgatccc catgccctgc gcgccgcatt tattgaagcc gacagccagg cggatgtagt
     7561 gatcagttcc ggcggtgttt cagtgggtga agcggattac accaaaacga ttcttgaaga
     7621 gctgggggag atcgccttct ggaaactggc gattaaaccg ggtaaaccgt tcgcgttcgg
     7681 taaactttct aatagttggt tctgcggcct gccgggcaac ccggtttcag cgacgctgac
     7741 cttctatcaa ctggtacagc ctttgctggc aaaactaagc ggcaataccg ccagtggcct
     7801 gcccgcgcgc cagcgtgtac gcacagcgtc ccgcctgaaa aaaacgccag gacgccttga
     7861 tttccagcgc ggcgtgctgc aacgcaacgc cgatggcgaa ctggaagtga cgaccaccgg
     7921 acatcagggt tcacatatat ttagctcctt tagcctcggc aactgcttta tcgtgctgga
     7981 acgcgatcgc ggcaatgtgg aagtgggcga atgggtggaa gtagaaccgt ttaacgcgtt
     8041 gttcggaggc ctgtaatggc ggaactcagc gatcaggaga tgctgcgcta caaccggcaa
     8101 atcattctgc gcggttttga ttttgacggt caggaagcat tgaaagattc ccgcgtgctg
     8161 gtcgtcggcc ttggtgggct cggctgcgca gcttcgcagt atctggcaag cgccggtgtc
     8221 ggcaacctga cgctgctcga cttcgacacg gtttcgctct ccaatctgca acgccagacg
     8281 ctgcacagtg atgccacggt cgggcaacca aaggtggaat ccgcccgtga cgccctgacg
     8341 cggatcaacc cacatatcgc gattacgcca gtcaatgcgc tgcttgatga cgctgaactt
     8401 gctgcgatga ttgcaaaaca cgatctggtg ctcgactgta cggataacgt tgcagtacgt
     8461 aatcaactga acgcaggctg ttttgccgcg aaggtgccgc tggtttccgg cgcggcaatt
     8521 cgtatggaag ggcaaatcac ggtcttcact tatcaggacg gcgaaccgtg ctatcactgc
     8581 cttagccgtt tgtttggcga gaacgcgtta acctgcgtag aagcaggcgt gatggcaccg
     8641 ttaatcggcg taattggttc gttgcaggca atggaagcaa tcaaactgct ggcaggttat
     8701 ggcaaaccag ccagcggaaa aatcgtgatg tacgatgcga tgacctgtca gtttcgcgaa
     8761 atgaaactga tgcgcaatcc ggggtgtggg gtgtgcgggc agtaactgcc acctccaggc
     8821 cagataagac gcggcactcc gccacgtcat cagcaattta tgaggatgca gaacgtccgg
     8881 ttaaatcgac gttctgccaa acgctcccag ccagtcctgt tcaaacttcg ccacagcggc
     8941 atcaaccgcc ggatagctaa tcatctgttg tgccacatcc agcggcagag taattgattc
     9001 acatcctgcc agtaagcagt ccagcgcctg acgcggggtt ttgaaactcg cggctaacac
     9061 tttcgcctgc ggcgcatgca ttttcaataa ctggtgtaag tcggtcacag tctgaatacc
     9121 gctaccgccc tgagcatcaa tacgattaac gtaaggcgca acatattccg cacctgccag
     9181 cgccgacagt agcccttgtg ctgcgccata taccgcggtt cccagcgtcg gaatcccttc
     9241 cgcttttaac atcttaatag ctgccagccc ctcagcggtc accggaacct tcaccacgat
     9301 atccgcaata atagaacgca atttacgtgc gtcattaacc atcccttcag cagtggtagc
     9361 cattacctgg gcaaacagac gcccctgacc gcccatcgcc tcgtgaagtt gcggaagcac
     9421 aacctccagc ggttttttac ccgctgcgat aatgcttggg ttagtggtca cacccgccag
     9481 cggaaaaata cgtgacagcg tcttcaccgc aacaacgtct gaagtatcca gatacagttc
     9541 cataaccatc ctcaaaatgt actttttgtc tgaatgtaga ctatcacggg aaaactctct
     9601 gaacagttga cgcacatcaa tctaactttc attcgaaagt attttaatgt ttgaacgaaa
     9661 cagagaggca actatgattt tcaatattca acgctactcg acccatgatg gccccggtat
     9721 ccgcacggtc gtatttctta aaggctgttc gctgggctgc cgctggtgtc agaacccgga
     9781 aagccgcgcc cgcacgcagg atctgctgta tgacccacga ctctgtctgg aaggctgtga
     9841 gctgtgcgct aagaccgcac cggaagtgat tgaacgcgcg ttgaatggtt tgcttattca
     9901 tcgggaaaag ttaaccccgg agcattttac ggcgctaacc gactgctgcc cgacacaggc
     9961 gttaaccgtg tgtggtgaag tgaaaagcgt tgaggagatc atggcaaccg ttttgcgcga
    10021 taaaccgttt tacgatcgca gcggcggcgg cttaacgctt tctggcggtg aaccgtttat
    10081 gcagccggaa atggcgacgg cgctgctgca agccagccac gaggccggca ttcatactgc
    10141 ggtagaaacc tgtctgcatg tgccgtggaa atatatcgcc ccttctctgc cctatatcga
    10201 tctgtttctt gccgatttaa aacacgttgc cgacgcgccg tttaaacagt ggaccgacgg
    10261 taacgccgcc agggtgctgg ataacctgaa aaaactcgcc gcagcgggca aaaaaatcat
    10321 tatccgcgtg ccactgattc agggctttaa tgccgacgaa acctctgtga aagccattac
    10381 cgattttgcc gccgacgagc tgcacgttag cgaaattcat ttcctgccct accacacgct
    10441 gggcatcaac aaatatcact tacttaatct gccctatgac gccccggaaa aaccgcttga
    10501 tgcgccagaa ctgctcgact ttgcccagca gtatgcctgc cagaaagggt taaccgcgac
    10561 cttacgagga taacaatcat gacaacacta aaactggata cgctcagcga ccgcattaaa
    10621 gcgcacaaaa atgcgctggt gcatattgtg aaaccgccgg tctgtaccga acgagcacaa
    10681 cactataccg agatgtatca acaacatctc gataagccaa tcccggtacg tcgcgcgctg
    10741 gcactggcgc atcacctggc gaatcgcacc atctggatca aacatgatga attgatcatt
    10801 ggcaaccagg caagcgaagt tcgcgccgcg ccgatcttcc cggaatatac cgtctcctgg
    10861 atcgaaaaag agattgatga tctggcagat cgtcccggtg ctggttttgc ggtgagcgaa
    10921 gagaacaaac gcgttctgca tgaagtgtgc ccgtggtggc gcggtcagac cgtacaggat
    10981 cgctgctacg gcatgtttac cgatgagcaa aaaggtctgc tggcgaccgg catcattaaa
    11041 gcggagggca atatgacctc cggcgatgcg cacctggcgg tcaatttccc gctactgctg
    11101 gaaaaaggtc ttgatggtct gcgtgagaaa gtggcggaac gtcgctcgcg catcaatctg
    11161 acggttctgg aagatctgca cggcgagcaa ttcctgaaag cgattgatat cgtgctggtg
    11221 gcagtcagtg aacacattga acgtttcgct gccctggcgc gtgaaatggc cgcaaccgaa
    11281 acccgcgaaa gccgtcgcga tgaactgctg acgatagcag aaaactgcga tcttatcgcc
    11341 caccagccgc cgcagacttt ctggcaggcg ctgcaactgt gctacttcat ccagttgatt
    11401 ttgcagatcg aatctaacgg tcactcggta tcgtttggtc gtatggacca gtatctctac
    11461 ccatactatc gccgtgacgt tgaactgaac cagacactgg atcgcgaaca cgccatcgag
    11521 atgctgcata gctgctggct gaaactgctg gaagtgaaca aaatccgctc cggctcacac
    11581 tcaaaagcct ctgcgggaag tccgctgtat cagaacgtca ccattggcgg gcaaaatctg
    11641 gttgatggtc aaccgatgga cgcggtaaat ccactctctt atgcgatcct cgaatcttgc
    11701 ggtcgcctgc gttcgactca gcctaacctc agcgtgcgtt accatgcggg tatgagtaac
    11761 gatttcctcg acgcctgtgt tcaggtgatc cgctgcggct tcgggatgcc ggcgttcaac
    11821 aacgacgaaa tcgtgatccc ggaatttatt aaactcggta ttgaaccgca ggacgcttac
    11881 gactacgcag caattggttg tatcgaaacc gccgtcggtg gcaaatgggg ctatcgctgt
    11941 accggcatga gctttatcaa cttcgcccgc gtgatgctgg cggcgctgga aggtggtcgt
    12001 gatgccacca gcggcaaagt gttcctgcca caagcaaaag cgttgtcagc gggtaacttc
    12061 aacaacttcg atgaagtaat ggacgcatgg gatacgcaaa tccgttacta cacgcgtaaa
    12121 tcaatcgaaa tcgaatatgt cgtcgatacc atgctggaag agaacgtaca cgatattctc
    12181 tgctcggcgc tggtcgatga ctgtattgag cgagcgaaaa gtatcaagca aggcggcgcg
    12241 aaatatgact gggtttctgg cctgcaggtc ggcattgcta acctcggcaa cagcctggcg
    12301 gcagtgaaga aactggtatt tgaacagggc gcgattggtc agcaacagct ggctgccgca
    12361 ctggcggatg acttcgacgg cctgactcac gagcagctgc gtcagcggct gattaacggt
    12421 gcgccgaagt acggcaacga cgatgatact gtcgatacgc tgctggctcg cgcttatcag
    12481 acctatatcg acgaactgaa gcagtaccat aacccgcgct acggtcgtgg tccgattggc
    12541 ggcaactatt atgcgggcac atcgtctatc tccgctaacg taccgtttgg cgcgcagacg
    12601 atggcaacgc cggacggacg taaagcacat accccgctgg cagaaggcgc tagtccggcc
    12661 tccggtactg accatctcgg ccctactgcg gtcattggct cggtgggtaa actgcctacg
    12721 gcagcaattc tcggcggcgt gctgctcaac cagaaactga atccggcaac gctggagaat
    12781 gaatctgaca agcagaaact gatgatcctg ctgcgtacct tctttgaagt gcataaaggc
    12841 tggcatattc agtacaacat tgtttcccgc gaaacattgc tggaggcgaa gaaacatccg
    12901 gatcaatacc gcgatctggt ggtacgcgta gcgggttatt ccgccttctt caccgcgctc
    12961 tctccagacg cacaggacga tatcatcgcc cgtaccgaac acatgctgta atcccctcag
    13021 cccggcgtcg tcgtcgccgg gccaaatcat tcacatcatc aatttcagcc tcgctttcat
    13081 tcgaaatata atttgtgctc tgcgtcacat tgttattaca ctgttacggt ttcattttcg
    13141 agccaggagc acacctatga gcgtaaaagt tatcgtcaca gacatggacg gtacttttct
    13201 taacgacgcc aaaacgtaca accaaccacg ttttatggcg caatatcagg aactgaaaaa
    13261 gcgcggcatt gagttcgttg tcgccagcgg taatcagtat taccagctta tttcgttctt
    13321 tcctgagcta aaggatgaga tctcttttgt cgcggaaaac ggcgcactgg tttacgaaca
    13381 tggcaagcag ttgttccacg gcgaactgac ccgacatgaa tcgcggattg ttattggcga
    13441 gttgctaaaa gataagcaac tcaattttgt cgcctgcggt ctgcaaagtg catatgtcag
    13501 cgaaaatgcc cccgaagcat tcgtcgcact gatggcaaaa cactaccatc gcctgaaacc
    13561 tgtaaaagat tatcaggaga ttgacgacgt actgttcaag ttttcgctca acctgccgga
    13621 tgaacaaatc ccgttagtga tcgacaaact gcacatagcg ctggatggca ttatgaaacc
    13681 cgtcaccagt ggttttggct ttatcgactt gattattccc ggtctacata aagcaaacgg
    13741 tatttcgcgg ttactgaaac gctgggatct gtcaccgcaa aatgtggtag cgattggcga
    13801 cagcggtaac gatgcggaga tgctgaaaat ggcgcgttat tcctttgcga tgggcaatgc
    13861 tgcggaaaac attaaacaaa tcgcccgtta cgctaccgat gataataatc atgaaggcgc
    13921 gctgaatgtg attcaggcgg tgctggataa tatcgctcct ttcaatagtt gatcttcccc
    13981 ctggccgaag tgaccacttc ggcctctctc ctttttgctc gctttgtgca ctgactcaat
    14041 atttttatct tgcgttaact tatttttaac ttaatgtatc aatcgcaatc attttacttt
    14101 cgaatgaaag aagcgaggtt gatatgactt tttcctgtga gacgctgccc acagatgcga
    14161 aagcagctat ccgagagatg aaagctgcgc tgcgggaaca gctcagtgat gtgcaacagg
    14221 ttttcgatac actctctgca aaaatcgaaa cgcgtgttgc tgaaatcgat gccctaaaag
    14281 cgcaagggct gccggtttgg ccggaagttt cttatagtga tattgccgca ggtacagtaa
    14341 gcgatgccac tcgcaatgaa attaaacgtc gcggttgtgc agtcataaaa ggccatttcc
    14401 ctcgcgagca ggcgatggcg tgggatcgtg ccatgctcga ttatcttgat atcaatcatt
    14461 ttgatgatgt ttataaaggc cctggcgaca gttttttcgg ctcgctggag gcttctcgtc
    14521 ccgaaatcta tcccatttac tggtcgcaag cacagatgca ggcgcggcag agcgaaaaca
    14581 tggctgctgt gcaatctttt ctaaaccgac tgtggaagtc tgaaagcaac ggcaaacagt
    14641 ggtttaatcc tgatatcagt gtgatttatc cagacaggat ccgccgccgc cctccaggta
    14701 ctacatctaa aggcctgggc gcgcataccg attctggtgc gctggaacgg tggttattac
    14761 ccgcttatca gcgcgttttt gccaatgtgt ttaatggtaa tttcgatgat tacgatccct
    14821 gggatgctgc acaccggacc gatgtcgaag agtacacggt agataacact acaaaatgtt
    14881 cggtgttccg cacgtttcag ggctggacgg cgctctctga tatgttatcg ggtcagggat
    14941 tgttacatgt ggtgcctatc ccggaagcga tggcgtatgt tttattgcgc ccgctgctgg
    15001 atgacgtacc ggacgatgaa ctgtgcgggg ttgcacctgg caaagttctg cccatttcag
    15061 agaaatggca tccactactg cttaaagcgc tgacttccat tcccgcgata acagcagggg
    15121 attccgtctg gtggcactgc gatgtgattc actccgtcgc gccagtagac aatcaacagg
    15181 gttggggcaa cgtgatgtat atccctgctg cgccaatgtg cgagaaaaat cgggcttatg
    15241 cacacaaagt caaaatggcg ctggaaaaag gcgcatcacc tggcgacttc ccgcgggaag
    15301 attatgaagc aaactgggaa ggccgcttta cgctggagga tctgaacatt cacggtaagc
    15361 gcgcactggg catggatgtt tgagagttaa tggaagtgaa ttgatgtggg attaagaaaa
    15421 tcgattcaac gttactcaga ttgctgacaa atgcgctttg ttaatgcctg atgcggcgtt
    15481 aacgccttat ccggcctaca aagtcctgca gattcaatat attgcaggta ctgcgtaggc
    15541 ctgataagca tagcgcatca ggcaatcttg cgtttgtcat cagccgccgt caccccgaca
    15601 gtatcgggct gacggataat aacttactcg atccctttac tacgcagata atcttcgtaa
    15661 ttaccgctaa agtcgatcac gcgttccggg gtgatttcca gaattcgggt cgccagggag
    15721 cttacaaact cacggtcgtg agacacaaag atcagcgtgc cctgatacag ttccagcgcc
    15781 atgttcagcg actcaatgga ttccatatcc aggtggttgg tcggttcgtc catgatcaga
    15841 atgttcggct tctgcatcat taacttacca aacagcatcc gccctttctc cccaccggaa
    15901 agcactttcg ctggcttttt gatgtcgtcc tggctgaaca gcaaacgacc gagaatgctg
    15961 cgtaccgcct gctcgtcatc gccttcctgc ttccactgac tcatccattc gaacacggta
    16021 agatcatttt cgaactcata ttcgtggtcc tgggcgtagt aaccaatgcg cgcgttttca
    16081 gaccatttta cggtgccgct gtccggttgc agatcgccca ccagcgtttt caacagtgtt
    16141 gatttaccga cgccgttggt acccagtacc gccagttttt caccgacttc cagcagcagg
    16201 ttgagatttt taaacagcgg accgttatca aaccctttgg tcagaccttc cacttccagc
    16261 gcgttacgga acagtttctt atcctgttca aaacggatga acgggttctg acggctggag
    16321 gctttcacct cttccagttt gattttatca atctggcgcg cgcgcgaagt tgcctggcga
    16381 gatttcgagg cgttggcact aaagcggcta acgaaagatt gcagctcagc aatctgcgct
    16441 ttcttcttgg cgttatcagc cagcagacgc tcacgcgcct gggtcgccgc cgtcatgtac
    16501 tcatcgtagt tacctggata aacgcgcagc tcgccgtaat ccagatccgc catgtgggta
    16561 caaaccatgt taaggaagtg acggtcgtgc gagatgatga tcatggtgct gtcacgctcg
    16621 ttcagcacct gttccagcca acgaatggtg tcgatgtcga ggttgttggt cggttcgtcg
    16681 agcaggagaa tgtccggatc agcaaacagc gcctgcgcca gcagcacacg cagcttccag
    16741 ccaggagcaa cttcactcat cgggccgtag tgctgttcca ctggaattcc cacgccaagc
    16801 aacagttcac cggcgcgagc ttccgcagag taaccgtcca tttcgccgta tttcacttcc
    16861 agatcggcca ctttatagcc gtcttcttca ctcatttccg gcaaagcata gatgcggtca
    16921 cgctcctgct tcacttccca caactcttta tgcccaatga tcaccgtatc cagcacagtg
    16981 aactcttcaa aggcaaactg atcctgacgc agcttaccaa tgcgctcgtt gggatcgagg
    17041 gaaacgttac ccaacgtcgg ctctaagtcg ccgccgagga tcttcataaa ggtggattta
    17101 ccactaccgt tcgcgccaat caggccgtaa cggttgccgc cgccaaattt gacggaaatg
    17161 ttttcaaaca acggcttact gccgaactgc atggtgacgt tactggaaac taacacaggc
    17221 gtatcctgaa aagagatatg acaaaccgcg cattatgcca taaatctgaa agaagatcct
    17281 gctcctcgct taaggtctac actcaagcca aaggcaaaaa atgtgatttc gtacacatct
    17341 gatttcactg tgagctggaa tgaacttata atgcgcttcc aatactctaa tattctcaac
    17401 ccaatggcct gccaggcaca aaatctcgct taacatgaat atgaaattga aaacattatt
    17461 cgcagcggcc ttcgctgttg tcggcttttg cagtaccgcc tctgcggtaa cttatcctct
    17521 gccaaccgac gggagtcgcc tggttggtca gaatcaggtg atcaccattc ctgaaggtaa
    17581 cactcagccg ctggagtatt ttgccgccga gtaccagatg ggactttcca atatgatgga
    17641 agcgaacccg ggtgtggata ccttcctgcc gaaaggcggt actgtactga acattccgca
    17701 gcagctgatc ctgccggata ccgttcatga aggcatcgtc attaacagtg cagagatgcg
    17761 tctgtattac tatccgaaag gaaccaacac cgttatcgtg ctgccgatcg gcattggtca
    17821 gttaggcaaa gacacgccta tcaactggac caccaaagtt gagcgtaaaa aagcaggccc
    17881 gacctggacg ccgaccgcca aaatgcacgc agagtaccgc gctgcgggcg aaccgcttcc
    17941 ggctgtcgtt ccggcaggtc cggataaccc gatgggtctg tatgcactct acatcggtcg
    18001 cctgtacgct atccatggca ccaacgccaa cttcggtatc ggcctgcgtg taagtcatgg
    18061 ctgtgtgcgt ctgcgtaacg aagacattaa attcctgttc gagaaagtgc ctgtcggtac
    18121 tcgcgtgcag tttattgatg agccggtgaa agcgactacc gaaccagacg gcagccgtta
    18181 cattgaagtc cataacccgc tgtctaccac cgaagcccag tttgaaggtc aggaaattgt
    18241 gccaattacc ctgacgaaga gcgtgcagac agtgaccggt cagccagatg ttgaccaggt
    18301 tgttcttgat gaagcgatca aaaatcgttc cgggatgccg gttcgtctga attaatcgtc
    18361 aaaacgtaaa acaaaggcgg actcataatc cgcctttttt atttgccaga ccctaccagg
    18421 ccgggatcat aaccaacaaa acatatccca ccagcgccgc ccacaacagc atcggtatcg
    18481 aataaagatg aaaacgccac cagatacggc gatcgttcgc catccgtaac gcaatcaaat
    18541 ttgccagcga tccgggtaat aaaccaaagc cccctacgtt taccgcccat gccagcagca
    18601 acgacggcgg cacatagttc agcaacaata tggtactcgg cacgttactg atcacctgcg
    18661 ataaaccgat tgccgttaac cataatccgg gttcggataa atgactcacg ttacttaaca
    18721 ccccctgcaa agctggcagt tgggttagta aatggacgtc gataaacatc gccataaaca
    18781 ccagcagcag cgtccagtcc acactgagca ccacgcgacg tgccagcagc gcaaagccca
    18841 ccgccacaat caccagtccc cacagctctt gttttaactc cagcgccatc agaaagacga
    18901 tatacagccc cagacaactc cacaccagcc gcggtttcca ctccggcgtt tgcgcccccg
    18961 tatggtattg cagcgccttt ccagggaaac aacaccagca caacagcagg agtgtcagca
    19021 tcattgcgcc agccagcggt gccatttggg caataaatcc ggcaaacgaa agaccagaac
    19081 gtccccagat aagaatattt tgcgggttgc caattggcgt cagtagcgaa ccagcgttga
    19141 ctgccagcgc ctcaaaaata atcagccgat taaccgggat ctcacacagt ctttttagcg
    19201 tgatagtcag cggaacaaca ataaacagcg cgacatcgtt ggtcagaaag gtagaaagca
    19261 gcgccgccgc cagcaccata aacatcgcca gccgacgctc cgtagcaaag cggcgcacca
    19321 ttttgcgccc cagcacatca aaataaccgc ttaactccac acctttggtc agcagcatca
    19381 ggccgcttaa ggtgatgatg gtgtgccagt cgatagcagc aggccaggat ttcggtgcaa
    19441 agggaacgaa aaaacttaat ccgatgccaa caagaattaa taactgaaaa aaacgatcgc
    19501 cttgcagcgt gcgtaaaaaa ggcaggctca tttggcaccg tgtttctggg taaacaaacg
    19561 aaaggcatcc agcgtctctt cactaacatg gtgctccatg ccttccgcgt cgcgacgggc
    19621 gatttccgga ctgacgccca acaccaacaa gaaattttcg actatctgat gtcgctcgcg
    19681 gctttcctgc gccagtttct ctccttctgc cgttaaaaac acgccacgcc aggggatcat
    19741 ttcaatcagc cccatggttg ccagtcgctt aagcatttta gccaccgtcg gttgcgaaac
    19801 tcccaaacga gcagccatgt ctacctgacg agcttccccc acttccctga tcaagtcaga
    19861 aatcagctca acgtaatcat caatcagctc gcgccgatgc gcctctctga cctggcggaa
    19921 cccttcaacg tgctcttcca cgttcactaa ctgcgtcact ttttttgctg ttggcgtacc
    19981 tgcgcgacga ctcattgtgc ttcctcatta tggtgacgca tacatagcgt tctcatttcg
    20041 tgcgcacatt gtaaaccaga gttgcgaagg tacaaaaaat taacgtttta gcaatagcta
    20101 tataatatag cctgtgctat atctgtatgt aatgcaatca tccctcaagg atcgacggga
    20161 ttagcaagtc aggaggtctt atgaatgagt tcaagaggtg tatgcgcgtg tttagtcatt
    20221 ctccctttaa agtacggtta atgctgctct ctatgttgtg cgatatggtc aacaacaaac
    20281 cgcagcaaga taaaccttca gataaatagc ggcgtcgcgg tacgccgctt cactcctgct
    20341 tttatgcagg cataacgcgt tttggtctga aaaaccccac tttttgtcgg atttgcaatc
    20401 cccttcgcaa aagatttgtt cgtcagtagt tgacctgaac ggcggctcgc tctatcttct
    20461 tgcagccctg cgtatattgc ggctcgcgga tgcggacccc tttcccctct ccacgcactc
    20521 ttgcaggtat tgacccttga cgccaaggta agcacatggc gtttgttacg atagtggcat
    20581 atgaatttaa ccctcaaaga atcgcttgtt acccgtagcc gggtatttag cccgtggact
    20641 gcgttctact ttttacagtc gctattaatt aacctcggct taggttatcc cttcagtttg
    20701 ctctacaccg ctgcgtttac ggctattttg cttttgctat ggcgaacatt gcctcgcgta
    20761 caaaaagttc tggtcggtgt cagttcgctg gtggcggctt gttatttccc ttttgctcag
    20821 gcctacggcg cacctaattt caatacattg ctggcattgc actccaccaa tatggaagag
    20881 tcgaccgaaa tcctgacgat ttttccgtgg tacagctacc tggtcggctt atttattttt
    20941 gcgctcggcg taatagcaat caggcggaaa aaagagagtg aaaaagcgcg ctggaatacc
    21001 ttcgacagcc tgtgtctggt attcagtgtg gcgacatttt ttgttgctcc cgtgcaaaac
    21061 ctggcctggg gtggcgtatt taaactgaaa gatactggct atccggtatt tcgttttgct
    21121 aaggatgtca tcgtcaataa taacgaggtg attgaagagc aagaacggat ggcaaaactg
    21181 tccggaatga aagatacctg gacggtcact gccgttaagc cgaagtatca gacctatgtg
    21241 gtggtgatcg gagaaagcgc gcgtcgcgat gccctcggtg cctttggcgg tcactgggac
    21301 aataccccgt ttgccagcag cgttaacggt ttgatatttg ctgactacat tgccgccagt
    21361 ggctccacgc agaaatcgct tggcttaacg ctcaatcgcg ttgtcgatgg caaaccacag
    21421 tttcaggata actttgtcac cctggcaaat cgcgcgggct tccagacctg gtggttttcc
    21481 aaccagggcc aaatcggcga atacgatacc gccatcgcca gcatcgccaa acgtgcagat
    21541 gaagtgtact tcctgaaaga aggtaatttt gaagcagata aaaacacgaa agacgaagcg
    21601 ttactggata tgaccgctca agtgctggcg caagagcact cgcaaccgca gctgattgtt
    21661 ttacatctga tgggctcaca tccgcaggcc tgcgaccgga cacaaggcaa atacgaaacc
    21721 tttgtgcaat cgaaagaaac gtcgtgctat ctctatacca tgacgcaaac agacgattta
    21781 ctgcgtaagc tgtacgatca gttacgcaac agcggcagta gcttctcgct ggtttacttt
    21841 tctgaccacg gtctggcctt taaagagcgt ggcaaagacg tgcaatacct tgcccatgat
    21901 gataagtatc agcaaaattt ccaggtgcct tttatggtca tttcgagcga cgataaagcg
    21961 catcgggtga ttaaagcccg ccgctctgcc aatgacttct taggcttttt ctcccagtgg
    22021 acgggaatta aagcgaagga aataaatatc aaatacccgt ttatatctga gaagaaagcc
    22081 gggccgatat acatcaccaa cttccagtta cagaaggtag attacaacca tctcggaacc
    22141 gatattttcg acccgaaacc ttaaaaacaa aaatccgccc cgagaggcgg attttttata
    22201 tcaccgaagt gattagaagc ggtaaccaac accggcaatc caggtgccta cgtcaacgct
    22261 acgaatacgg ctctgctcgt aagagaagtc cagagcaacg ttttccatcg ggttgaactg
    22321 cagaccagca ccgtaggaga aaccgtagtc gctggtgtcg tgtttgtagg tcgggtattc
    22381 agtggtctgg aatttaccat aacccacacc cactacaccg tagatgcttg cccagtcgtt
    22441 aatgcggtaa gccggaccag cagtgatgcc gtagtactgg tttttgttgt agtcaccaga
    22501 gcttgcagta cggcttttct cggtgtaagt gaaagaaccg atcacaccca gcgggctgtt
    22561 gtcttcttca tagcggtatt tcaggttgaa accgcccatt ttgttcattt ggccctgagc
    22621 gtcgctctgt gcgtaaccgc cagttacggt agaagtcgca gctacggaag tacctgcggt
    22681 gaaagccaga actgcggcca gtgctgaaag acatgcaatt tttttcataa ccacctcaaa
    22741 tgtgattcaa ataagtccta agttttaaat atatcaaaaa ttaatgggaa actcttcgcg
    22801 atttgtgatg tctaacgggc catttcatgt aacagaacgt ttccatacac cgctatccat
    22861 ctaaatttaa atcacttttt cagagagctg cataagtatt acgcatgttt tccctgtcat
    22921 tcatccagat tattcctaat caccagacta atgattccat caatcctggc gcattttagt
    22981 caaaacgggg gaaaattttt tcaacaaatg ctcaaccagc attgggtata tccagtacac
    23041 tccacgcttt acttaggttt agatatttgt gggagaaagg atgcctggtt cattacgtaa
    23101 aatgccggtc tggttaccaa tagtcatatt gctcgttgcc atggcgtcta ttcagggtgg
    23161 agcctcgtta gctaagtcac tttttcctct ggtgggcgca ccgggtgtca ctgcgctgcg
    23221 tctggcatta ggaacgctga tcctcatcgc gttctttaag ccatggcgac tgcgctttgc
    23281 caaagagcag cggttaccgc tgttgtttta cggcgtttcg ctggggggga tgaattatct
    23341 tttttatctt tctattcaga cggtaccgct gggtattgcg gtggcgctgg agttcaccgg
    23401 accactggcg gtggcgctgt tctcttctcg tcgcccggta gatttcgtct gggttgttct
    23461 ggcggttctt ggtctgtggt tcctgctacc gctggggcaa gacgtttccc atgtcgattt
    23521 aaccggctgt gcgctggcac tgggagcagg ggcttgttgg gctatttaca ttttaagtgg
    23581 gcaacgcgca ggagcggaac atggccctgc gacggtggca attggttcgt tgattgcagc
    23641 gttaattttc gtgccaattg gagcgcttca ggctggtgaa gcactctggc actggtcggt
    23701 tattccattg ggtctggcgg tcgctattct ctcgaccgct ctgccttatt cgctggaaat
    23761 gattgccctc acccgtttgc caacgcggac atttggtacg ctgatgagca tggaaccggc
    23821 actggctgcc gtttccggga tgattttcct cggagaaaca ctgacaccca tacagctgct
    23881 ggcgctcggc gcaattatcg ccgcttcaat gggttctacg ctgacagtac gcaaagagag
    23941 caaaataaaa gaattagaca ttaattaaat ttacatttct gcatggttat gcataaccat
    24001 gcagaattta tcgctacttt tcctctacac cgtctttata tatcgaatta tgcaaaagca
    24061 tatttattcc gaaaattcct ggcgagcaaa taaataagaa ttgttcttat taatatatct
    24121 aactcattga gtttttattg gttttgtttt ttcacgcttg ttaccactat tagtgtgata
    24181 ggaacagcca gaatagcgga acacatagct ggtgctatac ttagtctcgt taattactgg
    24241 gacataacat caagaggata tgaaattatg agtaccgcta aattagttaa atcaaaagcg
    24301 accaatctgc tttatacccg caacgatgtc tctgatagcg agaaaaaagc aacagtagag
    24361 ttgctgaatc gccaggttat ccagtttatt gatctttctt tgattaccaa acaagcgcac
    24421 tggaacatgc gcggcgctaa cttcattgcc gtacatgaaa tgctggatgg cttccgcact
    24481 gcactgatcg atcatctgga taccatggca gaacgtgcag tgcagctggg cggtgtagct
    24541 ctggggacca ctcaagttat taacagcaaa actccgctga aaagttaccc gctggacatc
    24601 cacaacgttc aggatcacct gaaagagcta gctgaccgtt acgcaatcgt cgctaatgac
    24661 gtacgcaaag cgattggcga agcgaaagat gacgacaccg cagatatcct gaccgccgcg
    24721 tctcgcgacc tggataaatt cctgtggttt atcgaatcta acatcgaata aatccatcgc
    24781 tgatggtgca gaactttagt acacgataaa ggcggcttcc tgacaggagg ccgttttgtt
    24841 ttgtagcccg cctcaacgca cttatttagt gcatccatct gctatcttca gctgattaag
    24901 taaatttttt gtatccacat catcacacaa tcgttacata aagattgttt tttcatcagg
    24961 ttttacgcta aataatcact gtgttgagtg cacaatttta gcgcaccagt ttggtgcccc
    25021 agaatggtgc atcttcaggg tattgcccta taaatcgtgc atcacgtttt tgccgcatct
    25081 cgaaaaatca atgagttgca aaactggcac gattttttca tatatgtgaa tgtcacgcag
    25141 gggatcgtcc cgtggataga aaaaaggaaa tgctatgaag tctgtattaa aagtttcact
    25201 ggctgcactg accctggctt ttgcggtttc ttctcatgcc gcggataaaa aattagttgt
    25261 cgcgacggat accgccttcg ttccgtttga atttaaacag ggcgataaat acgtgggctt
    25321 tgacgttgat ctgtgggctg ccatcgctaa agaactgaag ctggattacg aactgaagcc
    25381 gatggatttc agtgggatca ttccggcact gcaaaccaaa aacgtcgatc tggcgctggc
    25441 gggcattacc atcaccgacg agcgtaaaaa agcgatcgat ttctctgacg gctactacaa
    25501 aagcggcctg ttagtgatgg tgaaagccaa caataacgat gtgaaaagcg tgaaagatct
    25561 cgacgggaaa gtggttgctg tgaagagcgg cactggctcc gttgattacg cgaaagcaaa
    25621 catcaaaact aaagatctgc gtcagttccc gaacatcgat aacgcctata tggaactggg
    25681 caccaaccgc gcagacgccg ttctgcacga tacgccaaac attctgtact tcatcaaaac
    25741 cgccggtaac ggtcagttca aagcggtagg tgactctctg gaagcacagc aatacggtat
    25801 tgcgttcccg aaaggtagcg acgagctgcg tgataaagtc aacggcgcgt tgaaaaccct
    25861 gcgcgagaac ggaacttaca acgaaatcta caaaaaatgg ttcggtactg aaccgaaata
    25921 ataacgctac gcctgtaagg cgcactggca gttccctctc ccctctgggg agaggattag
    25981 ggtgaggggc gcaaacccgc tccggggcca ttaattaccc tgaatttgat tatttacacc
    26041 acggtaacag gaacaacata tgcagtttga ctggagtgcc atctggcctg ccattccgct
    26101 tctgattgaa ggtgccaaaa tgaccctgtg gatctcggtc ctcggtctgg ctggcggcct
    26161 ggtgatcgga ttgctggcag gttttgcacg caccttcggt ggttggatag ccaaccacgt
    26221 cgcgctggtc tttattgaag tgatccgcgg cacacctatc gtcgtccagg tgatgtttat
    26281 ctatttcgcc ctgccgatgg catttaacga cttacgcatc gacccattta ctgcggcggt
    26341 ggtcaccatc atgatcaact ccggcgcata cattgcggaa atcacgcgtg gtgcggtgct
    26401 gtctattcac aaaggttttc gtgaagcagg actggcgctc ggtctttcac gttgggaaac
    26461 cattcgctac gtcattttac cgctggcact gcgtcgtatg ctgccgccgc tgggtaacca
    26521 gtggatcatc agcattaaag acacctcgct gtttattgtt atcggcgtgg cggaactgac
    26581 ccgtcagggg caagaaatta ttgccggtaa cttccgcgcc cttgagatct ggagcgccgt
    26641 ggcggtgttc tatctgatta ttaccctggt gctgagcttt attctgcgtc gtctggaaag
    26701 aaggatgaaa atcctgtgat tgaatttaaa aacgtctcca agcattttgg tccaacccag
    26761 gtgctgcaca atatcgattt gaacattgct cagggcgaag tcgtggtgat tatcgggccg
    26821 tccggttccg gtaaatcgac cctgctgcgc tgcatcaaca aactggaaga aatcacttcc
    26881 ggcgatctga ttgtcgatgg cctgaaggtt aacgatccga aagttgacga gcgcctgatt
    26941 cgccaggaag caggtatggt gttccagcag ttttacctct tcccgcatct gacggcgctg
    27001 gaaaacgtca tgtttggtcc gctacgcgtg cgtggcgcga acaaagaaga ggcggaaaaa
    27061 ctggcgcgtg agctgctggc gaaagtcggt ctggcagaac gtgcacatca ctacccttcc
    27121 gaactttctg gtggtcaaca gcagcgtgtg gcgatcgcac gcgcgctggc ggtgaagccg
    27181 aaaatgatgc tgtttgatga accgacttcc gctcttgacc cggaactgcg ccatgaagtg
    27241 ctgaaggtta tgcaggatct ggctgaagaa gggatgacga tggtgatcgt gacacacgaa
    27301 atcggttttg ccgagaaagt agcttcgcgc ctgatcttta tcgacaaagg ccggattgcg
    27361 gaagatggcg atccgcaggt gttgatcaag aacccaccga gccagcgctt gcaggaattt
    27421 ttgcagcacg tctcttaact tctcagcctc cctttcctcc gaaagggagg cacaccagat
    27481 tcctctcatt taaaatcgcc cctcctccag catctatact tatctttttg ctctgttttc
    27541 tcactggagg agtcatgcgg tggatcctgt tcatcctctt ctgcctgctg ggcgcacctg
    27601 cccacgcggt atccataccc ggcgttacaa ccacaacgac aacggactca acgactgaac
    27661 cggccccgga accggatatc gaacaaaaaa aagcggccta tggtgcactg gcggatgtgc
    27721 tggataatga cacctcacgt aaagagttga tcgaccagtt gcgcaccgtc gccgcgacgc
    27781 cccctgcgga acccgtaccg aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc
    27841 tgcaaaaagt caccgaagtc agccgccatt atggtgaagc cctttccgcc cgcttcggac
    27901 aactttatcg caatatcacc ggctccccgc ataagccgtt taatccacaa accttcagca
    27961 atgcgttgac ccatttttca atgttagcgg tattagtgtt tggtttttac tggctgattc
    28021 gcctgtgcgc acttccgctg tatcgcaaaa tgggccagtg ggcgcggcaa aaaaatcgtg
    28081 agcgcagtaa ctggttgcag cttccggcga tgattatcgg ggcgtttatt atcgacctgc
    28141 tgttactggc actgacatta tttgtcggtc aggttttaag cgacaacctg aatgcgggca
    28201 gtcgcaccat cgctttccaa caaagtttgt ttctcaacgc ctttgccctg attgaatttt
    28261 tcaaagctgt actacgcctg attttttgcc caaatgtggc ggagctgcgc ccgttcacga
    28321 ttcatgacga gaccgcccgt tactggagcc gtcgcctgag ctggttaagc agtttgatag
    28381 gttatggcct gattgtggcc gtgccgatta tctctaatca ggtgaatgta caaattggcg
    28441 cgctggcgaa cgtcatcatt atgctgtgca tgaccatctg ggcgttgtac ctgatctttc
    28501 gtaataaaaa agagattacc caacatttgc tcaactttgc ggagcattcg ctggcctttt
    28561 tcagcttgtt tatccgcgcc tttgcgctgg tgtggcactg gctggcaagc gcctatttta
    28621 tcgtgctgtt tttcttttcg ttgttcgatc cgggcaacag cctgaaattt atgatgggtg
    28681 caacggtgcg cagcctggcg attattggta tcgcagcatt tgtttccggt atgttttccc
    28741 gctggctggc gaaaaccatc accctctcgc cacatactca gcgaaattat ccggagctgc
    28801 aaaaacggct gaatggctgg ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct
    28861 gcgtggcggt aatgctgctg ttgagcgcat ggggattatt cgatttctgg aactggctgc
    28921 aaaacggtgc ggggcagaaa accgtagata tcctgatccg tatcgcactc attcttttct
    28981 tctcggcggt tggctggacg gtgctcgcca gtttgatcga aaaccggctg gcttcggata
    29041 ttcatggccg cccactaccc agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg
    29101 cgctggcggt gattatcagt accatcacca tcatgattgt gttgtcggaa atcggcgtta
    29161 atatcgcgcc attgctggca ggtgccgggg cattaggtct ggctatctct tttggttcgc
    29221 aaacgctggt gaaagatatt atcaccgggg tatttattca gtttgaaaac ggcatgaaca
    29281 ctggagattt ggtgactatc gggccgttga ccggcactgt ggaacggatg tcgattcgct
    29341 ccgtgggcgt gcgccaggat accggggcgt atcacatcat tccgtggtct tcgataacca
    29401 cctttgctaa cttcgtccgc ggtattggtt cggtagtggc aaactacgat gttgatcgcc
    29461 atgaagatgc tgataaagcc aatcaggcac tgaaagatgc ggtagcagaa ttgatggaaa
    29521 acgaagaaat tcgcgggctg gttattggtg aaccgaactt tgccgggatt gtcggcttaa
    29581 gcaataccgc gtttacactg cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg
    29641 tccgctttgc cctcgacagc caggtgaaaa aacatttcga cctggcgggt gttcgcgcgc
    29701 cggtgcagac ttatcaggtg ctgccagctc cgggcgcggc cccggctgaa ccgttgccgc
    29761 cgggggagcc aacgctttaa cgctggcgat tgacaaaacg gcggcgctgc tcgtcgttca
    29821 taaaggtcca ggctataaag cggctctgct tttgcccctg ggccatctct ttttttacca
    29881 ccttcaccgc acctacgtcc gtcaaagcac gatacaacgg cggtaagttt tcaccacgag
    29941 ataccagcga ggtaaaccac atcacctgct tcgcgaagcc tttgctctct tcaatcatct
    30001 ttttgataaa agcgacctca ccgccttcac accacaactc ctgttgctga ccaccaaagt
    30061 tcagtgcatc gtctttgttc agccccaggt tacggcgttt acgttcacta cctgcccgtg
    30121 ccgcagcggc ggaatcgtgg aatggcgggt tacacaaggt cgcatcgtat tgctcgtttt
    30181 tatggatgat gccgttaaaa atcgccccac tctctttttg ccgacgcaga cgaatggcgc
    30241 ggttaagccc cggattagca ctgatgatcg cctgcgcact gcttaacgcc tggctgctgg
    30301 tttcactacc ggtaaaacgc cagccatatt catgtacgcc aatcagcgga taaatacagt
    30361 tcgcaccaac accgatatcc agaatgctgg cattagctgg aattgttccg cttgcttcag
    30421 ccagtaaatc ggcaaggtga tgaatataat ccgcccggcc cggtactggc gggcagagaa
    30481 aaccgtccgg gatatcccag ttcgctacgg cgtaaaaatg ggccagcaac gccttattga
    30541 gcgccttcac cgccagcgga ttggcaaagt ctacgctttg ctccccggcg ggtgtaagcg
    30601 tgaggaattg cctgagttca ggattgacct gacaaagcgt ggcgagatcg tagcggctgt
    30661 gatgacggtt gcgcggatgc aaccccggtt tctgggcgga catagcattc tcctttaagc
    30721 agcggcgtaa gatacccgca taacgcgaca gggtaaatat ctggctgcgt ttatcatttc
    30781 taaaaacctt cacatggcta tggtttatgg taattcacca taaaaatcat catcatgcat
    30841 gtcattgatt tatcttattt tgtaaaaata tattgttcaa aaaataagcg catctctgtc
    30901 aattttttgc gtcattttca tcgcgcaggc gacctacact tatctggcac tacccgttgt
    30961 atttatgagg atgtcattat gaaaaagtgc ctcacactac tgattgccac cgtcctgagc
    31021 ggaatctctc tcacggctta tgccgcgcaa ccgatgagta acctggacag tggtcaacta
    31081 cggcccgccg gtaccgtttc ggcaactggc gcatcaaacc taagcgatct ggaggataaa
    31141 ctggcagaaa aagcgcgcga acaaggcgcg aaaggttatg tcattaattc cgcaggcgga
    31201 aatgaccaga tgttcggtac tgcaatcatc tacaaataac cccaccaacg taactgcatt
    31261 cgcctctgga tgcagttttc gtccaatttg taagcaaaaa acatcaaata tgaacgcaat
    31321 gtaaataaat gtatttcttt ttcgcgcaat gggtgataga aaatcgcgcc aagtgataat
    31381 gcttatcaaa attattatca cttgcacgga cgctatcacg ggattaacag tggcatcgca
    31441 tccgcagaga tgctttctcg tgacagtgaa aatttcacca tataagaaaa agtcacctgc
    31501 aaaatggaaa acaatcgcaa tttccctgcc agacaatttc attcgctcac gttctttgcc
    31561 ggtctttgta ttggtattac gcctgtggcg caggcactcg ctgccgaagg gcaagctaac
    31621 gcggatgaca cgctggttgt cgaagcatca acgccttcgc tttatgcgcc acaacaatct
    31681 gccgatccga aattctcgcg tccggtagcg gatactaccc gcacgatgac ggtgatttct
    31741 gaacaagtga ttaaagatca gggtgcaacc aaccttaccg acgcgctcaa aaacgtcccc
    31801 ggcgtgggtg cgttttttgc gggtgagaac ggtaactcca ccactggcga cgccatttat
    31861 atgcgcggtg ccgatacctc taacagtatt tatatcgatg gcattcgcga tatcggcagc
    31921 gtctcgcgcg acaccttcaa taccgagcag gtcgaagtaa ttaaagggcc gtccggcacc
    31981 gactacgggc gcagcgcgcc gacaggctcg atcaatatga tcagcaaaca gccgcgcaat
    32041 gattccggca ttgacgcctc cgccagtatt ggcagcgcct ggttccgccg cggcacgctg
    32101 gacgtcaatc aggtcattgg tgataccact gcggtgcgcc tgaatgtaat gggcgaaaaa
    32161 acgcacgatg ccggacgcga caaagtcaaa aatgagcgtt acggcgtcgc cccttctgtc
    32221 gcttttggcc ttggtacagc gaatcgtttg tatcttaatt atctgcatgt cacccagcac
    32281 aacacgccag acggcggcat tccgactatc ggtttaccgg gttattctgc cccatctgcg
    32341 ggaacagcgg ctctgaatca ttccggaaaa gttgatactc ataactttta cggcacggat
    32401 tccgattacg acgattcgac caccgacacc gctaccatgc gttttgagca cgacatcaac
    32461 gataacacca ccattcgcaa tactacccgt tggtcgcgcg tgaagcagga ttacctgatg
    32521 acggcgatta tgggcggggc gtcgaatatt acccagccca ccagcgatgt gaatagctgg
    32581 acctggtcac gcacggcgaa tactaaagat gtgagtaata aaattctcac caaccagacc
    32641 aacctgacct cgacgttcta taccggcgct atcggtcatg atgtcagtac cggcgtggaa
    32701 tttacccgtg aaacgcagac taactacggc gttaatccgg tgacgttacc tgcggtaaat
    32761 atttatcatc ctgacagcag cattcatccc ggcggcctga cgcgcaacgg cgcaaacgcc
    32821 aatggtcaga cggatacctt cgcaatttac gccttcgata cgctgcaaat cacccgtgat
    32881 tttgagctga acggcgggat ccgtctggat aattatcata ctgaatatga cagtgccacc
    32941 gcctgcggcg gcagcggacg cggtgccatc acctgccctg ctggcgtggc aaaaggttct
    33001 ccggtcacca ccgtcgatac cgccaagtcg ggcaatctgg tgaactggaa agccggggcg
    33061 ctgtatcacc tgacggaaaa cggcaatgtc tatattaact atgccgtttc ccagcagcct
    33121 ccgggcggca acaacttcgc ccttgcgcag tctggcagcg gtaacagtgc caaccgcacc
    33181 gattttaaac cgcaaaaagc caacaccagc gagattggca ccaaatggca ggttctggat
    33241 aaacgcctgt tgctcaccgc cgcgctgttc cgtactgata tcaaaaatga agttgagcaa
    33301 aatgatgacg gaacttactc gcaatacggt aagaaacgcg tcgaaggcta tgagatatcc
    33361 gtggccggga atatcactcc cgcgtggcag gtgattggcg gctataccca gcaaaaagca
    33421 accatcaaaa acggcaaaga tgttgcccag gatggttcct catcgctgcc gtataccccg
    33481 gagcacgcct ttaccttatg gagccagtat caggcaaccg atgatatttc cgttggtgcg
    33541 ggcgcacgct atatcggcag tatgcataaa ggttctgacg gcgcggtggg aacgccagcg
    33601 tttaccgaag gttattgggt cgccgacgct aaactggggt atcgggttaa tcgcaatctc
    33661 gacttccagc taaacgtcta caacctgttt gataccgatt acgtcgcctc aatcaacaag
    33721 agcggctacc gttatcaccc gggcgagcca agaaccttct tgctcacggc caatatgcat
    33781 ttctgattga gatgtggggc gcaggcccca ctttttggag aacttgtatg atgtaccaca
    33841 ttcccggcgt gctatcgcca caggacgtcg cccgttttcg cgaacaactg gaacaagccg
    33901 aatgggtgga tggacgcgtc accactggcg cacaaggtgc gcaagttaag aacaatcaac
    33961 aggtagacac ccgcagcacg ttatacgccg ccctgcaaaa tgaggtgctg aacgcggtta
    34021 accaacatgc tttgttcttt gccgcggcct tgccgcgtac cctttccacg ccgctgttta
    34081 atcgctatca gaacaatgaa acctatggtt ttcatgtgga tggcgcagta cgcagtcacc
    34141 cacaaaacgg ctggatgcgc actgaccttt ctgccacgct gtttttaagc gatccacaaa
    34201 gctacgatgg cggcgaactg gtcgttaatg acaccttcgg gcaacatcgg gtaaaacttc
    34261 cggcaggcga tctggtgttg tatccctcca gcagcctgca ttgcgtgaca cccgtaaccc
    34321 gcggcgtacg agtggcatca tttatgtgga tccagtcgat gatccgcgat gataaaaagc
    34381 gcgccatgct gtttgaactg gacaacaata ttcagtcgct gaaaagccgc tacggcgaaa
    34441 gtgaagagat cctgtcgctg ctcaatcttt atcataatct gctgcgggaa tggtcggaga
    34501 tctgacgctt gaactgcccg agttcgtccc tatattagct acacttaact gtacaagtat
    34561 tgatatgggg aggacgatat ggcatccggt tgggctaacg atgacgccgt caacgaacag
    34621 atcaacagta caattgaaga tgcgattgcc cgcgctcggg gtgaaattcc gcgtggcgaa
    34681 agcctgtatg aatgtgaaga gtgcggagcc cccatcccgc aagcccgtcg ggaagccatt
    34741 cctggtgtgc gcttatgcat tcattgtcag caggagaaag atttacaaaa accagcttat
    34801 acaggatata atcgcagagg ttcgaaagac agccagttac gttaactaca actggcggga
    34861 tgtatagcga gtataatttg tattttgcgt ggcattccgt gcctttaacg ctgtcgtgac
    34921 tgccacgctg cataaaacaa gtccataagt tcaacaaata aatggattat cagtatgtta
    34981 gatatcaatc aatttatttg aacaaggcgg tcaattctct tcgattttat ctctcgaaaa
    35041 aaaacgtgat actcatcaca tcgacgaaac aacgtcactt atacaaaaat cacctgcgag
    35101 agattaatta tgaaaactat caatactgtt gttgctgcta tggctctttc aactctgtca
    35161 tttggcgtgt ttgccgcgga accggtaacg gcatcccagg cacagaacat gaataaaatc
    35221 ggcgtggttt ctgccgatgg cgcatccacc ctcgatgccc tggaagcgaa actggctgag
    35281 aaagctgcgg cagctggggc gagtggatac agcatcactt ctgccaccaa caacaataaa
    35341 ttaagcggta ctgcggtaat ttataagtaa ttcgtagaac ggataaggcg tttacgctac
    35401 gtccagcaca agtatttccc tgtctgtttg ccgacagacg catatgctct aaccctcatt
    35461 gatcctatgt tacccttgtt tgcccgtccg ccactggacg ggcttttttt tagctggcta
    35521 actgctgaca gaaagcctgc aacgtctctt ccggcatacc aacctttcgc gccgcatcac
    35581 aaatggcctg ccagcttccc gcatccaatg gaatcccctg ctcctgccgt tcgcgacgcg
    35641 tgttaacttc ccactcgccc ggtagcaaaa tcggcttatc gtcatcatgc ggcgaggctt
    35701 tcacccactc ggcaaaggct tcggtctgcg cgctacaatc cggcgcgccg aacagttccg
    35761 ggttgatgat gatagtggtc atgcagttaa gaatggcatc gggactggtt tgtaacgttt
    35821 tctgatgcgt cgttttaccg ccggaaagcg ccccaccaag aatttcacac atcgctgcaa
    35881 gggcgtagcc tttatgttcg gcaaaggtca acagcgaacc caacggcgac tcctgcatta
    35941 ccgccggatt ggtcgtcggc atgccgttaa cgtcaatcag gcaacctggc ggcacgggga
    36001 cgcctttatg ccaggcgacg cgggttttgc caaatgcgat ggcgctggtg gcgtaatcaa
    36061 gcaacagcgg gaaattatct ttacgaggga aaaccacaca gaacggattg gtgccaaagc
    36121 ggctgtcgcg accgtggaac ggcgcgacca ttggaatacc gaccacgcta acaaagtgga
    36181 tagagacaaa ccccgccgct gcacactgct ccgcccagta accgatacgg ccgatatgat
    36241 gcgagttatg tagcgccacg gcagcaatac cgtgctgatg cgctttctca atccccagcg
    36301 ccatcgcttc gtgtgccgcg acctgaccaa atgcgcgatc gccatcaagc gtaaccaccg
    36361 cgcccgcttc tttaacgatt ttggcatggt ggttaatttg cagatgcccc tgactccagg
    36421 aacgcacata gcttgggatc atgccaatac catgtgaatc atgccctgcc aggtttgccg
    36481 cgattaaatg atcggcaact aatttcgctt cttgttcctc gctacccatc tgacgaaata
    36541 cagcctgaat aaaactgtgc agcgtctgag catcaaagcg atgaccactt tccatggttt
    36601 actcctgttt atgttatgtg tttgttgttt ttttatgctg cacgccgggc gcgggaaggt
    36661 caataaagat gcaggaattt catattgcgg tggggtaaaa gccggatgac atggctcatc
    36721 cggctgagat aaaaattaaa atgcctgatt aatgcgcgcc aggccgtcgt tgatagttgc
    36781 cgcttcaccc gtagccatca ggcagcaagc catctggatt ttcagcgatt caggaatcgg
    36841 ttcgctgcca gcaaggcaac gcttaatcca ctgcgccgtg gtttccggat cttttgcttg
    36901 tggcagtaac tcgctaccag cagtgtcctg tttttcatac agtacccgca tcccttcacg
    36961 gtcaatgaga ttgatctgcg ggcagcgctg cggattagca tacacttcac cttcagtacc
    37021 atgcattagt agcgcccgcc cgccgatatc actaaagaac ttcgcgacgc gtccaatgta
    37081 ttccggatgc gaaacgctgg aaagacgcag tgcctcacct tcggcaaatg gcgtcgccag
    37141 tttcgccagg gtatgtgcac tgttacgcac gcccatccgc cagcgcatcg ccagttgttt
    37201 ttccagcggc gggcaaaacg cgccgacagg cataaacacc ggttgatgtt cgtcgagctt
    37261 cgcctgcgcc tgcccgccgt gcaacgttgg cgtaataccc atcagttcga aaatggtttc
    37321 tgtcagcacg cgggttggat cttcgctaac cccgtgaacc accacaggaa aaccgagttt
    37381 gtgcaggaga atcgccaaca acggcgtcag gttggcctgt ttacgcgcgc cgttgtaact
    37441 ggggatgaca atcggcatcg gcttgtctgc aggcggcgtc agcttgatgg tgtgattttg
    37501 catggcttcg taaaagccga gcatctctgc ttccccttcc cctttgatac gcagcgcaat
    37561 caatacgccg cccaactcaa ggtcagggac ttcaccattg agcatatggg catacagacc
    37621 gcgcgcagta tcccggtcta aatcgcgcgc gtggtttttc ccgcgcccga tctctttaat
    37681 gattttgcga tagtccattt acgactcctt acctgactca catcattaac gccgccgacg
    37741 gcgtggggat ttcgtttttt ctttcttttt aactatacct tcagggactt ccggttgctc
    37801 tatcggaaat accggtaatg catccagtag tcgcttgcca taatttttgg tcagcaagcg
    37861 tttgtcgtag ataaccactt ccccccagca accgtggctt cgaatcagtc gcccaacctg
    37921 ctgaatcagg ttaaacgaag cgctcggcag gctttgcacc tcaaacggat agcggttgag
    37981 gcttttcagc cattcacctt cggtgatcac caccggactg tcgatgggcg gaaaagcgat
    38041 cttgtggata tgcacctgac tgagcagatc gcctttcaga tcaagacctt cggcaaagga
    38101 ctgtaagccc accagcacgc tacgctcacc gttggcaacg cgtttgcggt gcagttcaac
    38161 taagcgataa cgtggctgat cgccctgcac cagcaacatc aggcgtaaat ccgtcacata
    38221 gtcgagaaag cgctgcatcg cacgcccgct ggcaaacagt accaacatac cgagatgttt
    38281 tttgctctcc acctgctcac ggaaaaaggc tgccatttca gcaatgtgct gctcttcgtt
    38341 gtcgatggaa ggctcaaagc gcatccgggg aataacaatt ttgccctgtt cgcagtggtt
    38401 aaagggcgaa tccagcgcca caaagcggtc gcccgctttc tctttcagac cactcatctc
    38461 ctgtaaacgc gaaaaactgt tcagcgaacg taaggtcgcg gaagtgacaa taatgtgcgg
    38521 aatactgcgc cacagcagcc tttccagctg atcgctgaca cgaataccca cacagtgaaa
    38581 ccagagatgc agctgccctt cgcgctcttc ccgcgtcgcc catttagtca ccggcgcacc
    38641 ggaagattgc gccagagacg ccagccgcca gagtttgctt tgcgcctcga acatccccaa
    38701 cgcgcgattc atctgcaaaa tcaaccgatg cagacgtaca atgtcatggc taccggtttt
    38761 ctcacttaaa tcgttaagaa ataactccgc caggccacgc agcatctcgg tgagttttgc
    38821 cagccgctgg cagatctcca gcacttcatc aggcagttcg cccatcgcaa aacggtgctc
    38881 tgcctcctgc ccggcaggca tgtagagatt gagaatgttg tttaacgagg cgataagctc
    38941 atacagctct tcacaatgcg cattcaaacg ttcagggatc gccagcggtg ggatggtctt
    39001 cgggcgaaac tgctccatgc aggtagcgac cagtttcgtg aacaagtcca gctgtagccg
    39061 ataccagggc gcggtgattt cggcgctcat ctccagcgca tcccgcgcca catctggcag
    39121 gtgatgacct tcgtccagca ccagcagcag atttttcggg tcaggtagta cggcttcgct
    39181 ttccatcgcc gccatcacca gcgcatggtt tgccaccacc acttccgctt cctgaatctc
    39241 ccgacgagca acaaaaaacg ggcattcacg atagtagtaa cagttgcggt tgaggcagct
    39301 ggctttgtcg gtacttaaac gacgccagag atcgtcatca atggcgatat ccgtatgatc
    39361 gcgcagacca tcccatttat aagtgtcgag atcgcccttc agcttcgcac aacgtttttg
    39421 ctcttcctga ttgttcggcg tcagctcgtc gtcaagaaac gccagcagat cctgttgcgt
    39481 gggttcagta ctggcaagcg ctgtcagatt acgcggacaa acgtagcgcc cacgcccaaa
    39541 agcagcagtg aatttaagat cgggaatgat ctttttcagc agaggcaaat ctttactgta
    39601 gatctgatcc tgcaatgcca cgttggcggt actcaccacc agcgtttttt gctcttcgcg
    39661 ggcaatggcg atgccgggaa tcaaatagga gagcgttttc ccaacgccgg tgggtgcttc
    39721 aatcgccaga tgccgccctt cttctccggc cagcgttttg gcgacgtccg caatcatctg
    39781 ccgctgcggc gcacggggaa taaagtcggg gatctgttcc tgaagcgcct tataccaggc
    39841 ggcaatttgc gctttaagcg cggcggttaa tgccatgaga aaacctgaaa tactgtataa
    39901 acagccaata ttgtggcatt tttcggtttt caggggtaat ggtttttctg tttctggaaa
    39961 gcggctcgca ataaccagca ggatgatgcg taaagcgctt cgcgctaaat tcgaacaaca
    40021 tgcagaactg cgtacactct tgctggcaac cgcgtccgca aaactggttg agcatacgca
    40081 aaatgatgct tactggggag atagcggtaa tggtcagggc aagaatcgcc tggggtacct
    40141 tttaatggcg ttgcgcggac aattggctgc ggagaagtaa cgcatcgggt ggcttgtggg
    40201 cgttaaaaca gagaaatctt tttgccgcgc tcgcaaatgg ctgacaagtg cttgttgtga
    40261 aatgccggat gcggcgtaaa cgccttatcc ggcctacaaa agcatgcaaa ttcaatatat
    40321 tacagagttc acgtaggcct gataagcgca gcgcatcagg cagttttgcg tttgttcatc
    40381 agcctgatgc cgggcatcgc ccggcatcaa aagattactg cgcagcggca ggtttacgtg
    40441 gacggcggcg acgttgttgt tcgcctgccg gtttggcatc gccgaggcgg cgagacggtt
    40501 tttctgcagg tttcgcactt gcagattttg cgccaccttc tccacggcgt ggttgctgtt
    40561 gaccgcgacc accaccttgc ccacgaccgc cgccacgttg ctggcgaccg ttctggatcg
    40621 gttcggcttt gattgacggg tccggctcat aacccggaat cgcaatgcgc gggatctctt
    40681 ttttcagcag tttttcgata tcacgcagca gtttgtgttc atcaacacac accagcgaca
    40741 acgcttcacc ggtagcagcc gcacgaccgg tacgcccgat acggtggaca taatcttccg
    40801 gtacgtttgg cagttcatag ttgaccacgt gcggcagctc ttcaatatcc aggccgcgcg
    40861 cagcgatgtc agttgccacc agtacacgaa tatcgcccga tttaaaatca gccagcgcac
    40921 gagtacgcgc accttgcgat ttattaccgt ggatcgccgc actacggatg ccatctttat
    40981 taagctgttc agccagatgg ttagcgccat gtttggtacg ggtaaacacc agcacctgct
    41041 gccagttccc tttcccaatc atatgcgaca gcaattcgcg tttgcgtttc ttatcgacaa
    41101 agtgaacgtg ctgggtcacc tgatcagacg cggtattgcg gcgtgccact tcgatttcca
    41161 gcgggttgtg caacagtttt tccgccaggg ctttaatatc gtcagagaag gtcgcggaga
    41221 acaacaggtt ctggcgcttc gctggcagtt ttgttaacac gcgacggata tcgtggataa
    41281 agcccatgtc gagcatgcgg tcagcttcat caaggacgag gatttcaacc tgatccagct
    41341 tcactgcatt ttgatgttcc aggtccagca gacgtcccgg cgttgccacc agcacatcaa
    41401 cgccgccgcg caacttcatc atttgtgggt taatgctgac accgccaaac accaccagcg
    41461 aacgaatgtt cagatatttg ctgtaatcac ggacgttttc gccaatctgc gccgccagtt
    41521 cacgggtcgg ggtaagaatg agcgcgcgta ccggacgacg ccctttggcg tgcggctggc
    41581 gagtgatcag gtgttgcaac agcggcagcg taaagcccgc tgttttgccg gtaccggtct
    41641 gggcgctggc catcaggtcg cggccttcca gcaccgcagg gatcgcctgc tgctgaatag
    41701 gggtgggttc acggtaaccc tgctcggcaa cggcgcgcag gatatcaggg cttaaaccca
    41761 aagaatcgaa agacataact actccgaacc gccccgaccg ttaccggtgt agttttcagg
    41821 gagatactga aaaggaaaat gacaaaaacc acaatgtcat gagggagcgg agtgtagcag
    41881 tttttgtgac gcagcgcata aattatccct gtgactggcg ttgctaaaat tcttagccat
    41941 actggacaac tctcatatgg agtcataatc ttaatcaatc atttgattaa gaatggactg
    42001 tgcgatgaat aatcctgcca tgacaatcaa gggtgaacag gcgaagaaac agctgattgc
    42061 tgccgcactg gcgcagtttg gtgaatatgg aatgaacgcc accacccgcg agatagccgc
    42121 ccaggccggg cagaatatcg ctgccatcac ctattacttc ggttcgaaag aggatttgta
    42181 cctcgcctgc gcccagtgga ttgccgattt tattggcgag cagttccgtc cacacgccga
    42241 agaagccgaa cgtttgttcg cgcaaccaca gcctgatcgc gcagctatcc gtgaactgat
    42301 ccttcgcgcc tgcaggaaca tgattaagct gctcacccag gatgacaccg tcaacctcag
    42361 caaatttatc tcccgtgagc agctctctcc cacggcagcc tatcacctgg tgcacgaaca
    42421 ggtgattagt ccgctacaca gccaccttac acgcctgatt gccgcctgga ccggctgcga
    42481 cgctagcgat acccgcatga tccttcatac ccatgcgttg attggtgaga ttctggcgtt
    42541 tcgtcttggc aaagaaacta ttctgttacg taccggctgg accgcgttcg atgaagaaaa
    42601 gaccgaactg atcaaccaga cggtgacctg tcatattgac ctgattttgc aaggattatc
    42661 gcaaaggagt ttgtagtgat gaaaaaacct gtcgtgatcg gactggcggt agtggtactt
    42721 gccgccgtgg ttgccggagg ctactggtgg tatcaaagcc gccaggataa cggcctgacg
    42781 ctgtatggca acgtggatat tcgtacggtg aatcttagtt tccgtgttgg ggggcgcgtt
    42841 gagtcgctgg cggtggacga aggtgatgcg atcaaagcgg gccaggtact gggcgaactg
    42901 gatcacaagc cgtatgagat tgccctgatg caggcgaaag cgggcgtttc ggtggctcag
    42961 gcgcagtatg acctgatgct tgccgggtat cgagatgaag aaatcgctca ggccgccgca
    43021 gcggtgaaac aggcgcaagc tgcctatgac tatgcgcaga acttctataa ccgccagcaa
    43081 ggactgtgga aaagccgcac catttcggca aatgacctgg aaaatgcccg ctcctcgcgc
    43141 gaccaggcgc aggccacgct gaaatcagcg caggataaat tgcgtcagta ccgttccggt
    43201 aaccgcgaac aggacatcgc ccaggcgaaa gccagcctcg aacaagcgca ggcgcaactg
    43261 gcgcaggcgg agctgaattt acaggactca acgttgatag ccccgtctga tggcacgctg
    43321 ttaacgcgcg cggtggagcc tggcacggtg ctcaatgaag gtggcacagt ctttaccgtc
    43381 tcactgacac gtccggtgtg ggtacgtgct tatgttgatg aacgtaatct cgaccaggcc
    43441 cagccggggc gcaaagtgct gctttatacc gacggtcgcc cggacaaacc gtatcacggg
    43501 cagattggtt tcgtttcgcc taccgctgaa tttaccccga aaactgtcga aacgccggat
    43561 ctgcgtaccg acctcgtcta tcgcctgcgt attgtggtga ccgacgccga tgatgcctta
    43621 cgccagggaa tgccggtgac ggtgcaattc ggtaacgagg caggacatga atgatgccgt
    43681 tattacgctg aacggtctgg aaaaacgctt tccgggcatg gacaagcccg ccgtcgcgcc
    43741 gctcgactgt accattcatg ctggttatgt aacggggctg gtggggccgg atggcgcagg
    43801 taaaaccaca ctgatgcgca tgctggcggg attactgaaa cccgatagcg gcagcgccac
    43861 ggtgattggc tttgatccga tcaaaaacga cggcgcgctg cacgccgtgc tcggctatat
    43921 gccgcagaaa ttcggcctgt atgaagatct gacggtgatg gagaacctca atctgtacgc
    43981 ggatttgcgc agcgtcaccg gtgaagcgcg gaagcagact tttgctcgcc tgctggaatt
    44041 tacatctctt ggcccgttta ccggacgcct ggcgggcaag ctctccggcg gaatgaagca
    44101 aaaacttggc ctggcctgta ccctggtggg cgaaccgaaa gtgttgctgc tcgatgaacc
    44161 cggcgtcggc gttgacccta tctcacgccg cgaactgtgg cagatggtgc atgagctggc
    44221 aggcgaagga atgttaattc tctggagcac ctcgtatctc gacgaagccg aacagtgccg
    44281 tgacgtgtta ctgatgaacg aaggcgagct gctgtatcag ggagaaccga cggccctgac
    44341 tcaaactatg gccggacgca gctttctgat gaccagcccg cacgagggca accgcaaact
    44401 gttgcaacgg gcattgaaac tgccgcaggt cagcgacggc atgattcagg ggaaatcggt
    44461 acgtctgatc ctcaaaaaag agaccacgcc agacgatatt cgccatgccg acgggatgcc
    44521 ggaaatcaac atcaacgaaa ctacgccgcg ttttgaagat gcgtttattg atttgctggg
    44581 cggtgccgga acctcggaat cgccgctggg cgcaatatta catacggtag aaggcacacc
    44641 cggcgagacg gtgatcgaag cgaaagagct gaccaaaaag tttggcgatt ttgccgccac
    44701 cgatcacgtc aactttgccg tcaaacgcgg ggagattttt ggtttgctgg ggccaaacgg
    44761 cgcgggtaaa tcgaccacct ttaagatgat gtgcggtttg ctggtgccga cctctggtca
    44821 ggcgctggtg ctgggcatgg atctgaaaga gagttccggt aaagcgcgcc aacatctcgg
    44881 ctatatggcg caaaaatttt cgctctatgg caacctgacg gtcgaacaga atttacgctt
    44941 tttctctggt gtgtatggct tacgcggtcg ggcgcagaac gaaaaaatct cccgtatgag
    45001 cgaggcgttc ggcctgaaaa gtatcgcctc ccacgcgacc gatgaactgc cattaggttt
    45061 taaacagcgg ctggcgctgg cctgttcact gatgcatgaa ccggacattc tgtttctcga
    45121 cgaaccgacg tccggcgttg atcccctcac ccgccgtgaa ttttggctac atatcaacag
    45181 tatggtagag aaaggcgtca cggtgatggt caccacccac tttatggatg aagcggaata
    45241 ttgcgaccgc atcggcctgg tgtaccgcgg gaaattaatc gccagcggca caccggacga
    45301 tttgaaagcg cagtcggcca acgatgagca acccgatccc acgatggagc aagcctttat
    45361 tcagttgatc cacgactggg ataaggagca tagcaatgag taatccgatc ctgtcctggc
    45421 gtcgcgtgcg ggcgctgtgc gtcaaagaga cgcggcagat cgttcgcgat ccgagtagct
    45481 ggctgattgc ggtagtgatc ccactgctac tgctgtttat ttttggatac ggcattaacc
    45541 tcgactccag caagctgcgg gtcgggattt tactggaaca gcgtagcgaa gcggcgctgg
    45601 atttcaccca caccatgacc ggttcgccct acatcgacgc caccatcagc gataaccgtc
    45661 aggaactgat cgccaaaatg caggcgggga aaattcgcgg tctggtggtt attccggtgg
    45721 atttcgccga acagatggag cgcgccaacg ccaccgcacc gattcaggtg atcaccgacg
    45781 gcagtgagcc gaataccgct aactttgtgc aggggtatgt cgaagggatc tggcagatct
    45841 ggcaaatgca gcgagcggag gataacgggc agacttttga accgcttatt gatgtacaaa
    45901 cccgctactg gtttaacccg gcggcaataa gccagcactt tattatcccc ggtgcggtga
    45961 ccattatcat gacggtcatc ggcgcgattc tcacctcgct ggtggtggcg cgagaatggg
    46021 aacgcggcac catggaggcg ttactctcta cagagattac ccgcacggaa ctgctgctgt
    46081 gtaaactgat cccttattac tttctcggga tgctggcgat gttgctgtgt atgctggtgt
    46141 cagtgtttat tctcggcgtg ccgtatcgcg gttcgctgct gattctgttt tttatctcca
    46201 gcctgttttt actcagtacc ctggggatgg ggctgctgat ttccacgata acccgcaacc
    46261 agttcaatgc cgctcaggtc gctctgaacg cggcttttct gccgtcgatt atgctttccg
    46321 gctttatttt tcaaatcgac agtatgcccg cggtgatccg cgcggtgacg tacattattc
    46381 ccgctcgtta tttcgtcagc accctgcaaa gcctgttcct cgccgggaat attccagtgg
    46441 tgctggtggt aaacgtgctg tttttgatcg cttcggcggt gatgtttatc ggcctgacgt
    46501 ggctgaaaac caaacgtcgg ctggattagg gagaagagca tgtttcatcg cttatggacg
    46561 ttaatccgca aagagttgca gtcgctgctg cgcgaaccgc aaacccgtgc gattcttatt
    46621 ttacccgtgc tgattcaggt gatcctgttc ccgttcgccg ccacgctgga agtgaccaac
    46681 gccaccatcg ccatctacga tgaagataac ggcgaacact cggtagaact tactcaacgt
    46741 tttgcccgcg ccagcgcctt tacccatgtg ctgctgttga aaagcccgca ggagatccgc
    46801 ccaaccatcg acacacaaaa ggcattactc ctggtgcgtt tcccggctga cttctcgcgc
    46861 aaactggata ccttccagac cgcgccgttg cagttgatcc tcgacgggcg taactccaac
    46921 agtgcgcaaa ttgccgccaa ctacctgcaa cagatcgtca aaaattatca gcaggagctg
    46981 ctggaaggaa aaccgaaacc taacaacagc gagctggtgg tacgcaactg gtataacccg
    47041 aatctcgact acaaatggtt tgtggtgccg tcgctgatcg ccatgatcac cactatcggc
    47101 gtaatgatcg tcacttcact ttctgtcgcc cgcgaacgtg aacaaggtac gctcgatcag
    47161 ttactggtct caccgctcac cacctggcag atcttcatcg gcaaggccgt accggcatta
    47221 attgtcgcca ccttccaggc caccattgtg ctggcgattg gtatctgggc gtatcaaatc
    47281 cccttcgccg gatcgctggc gctgttctac tttacgatgg tgatttacgg tttatcgctg
    47341 gtgggattcg gtctgttgat ttcatcactc tgttcaacac aacagcaggc gtttatcggc
    47401 gtgtttgtct ttatgatgcc cgccattctc ctttccggtt acgtttcgcc ggtggaaaac
    47461 atgccagtat ggctgcaaaa cctgacgtgg attaacccta ttcgccactt tacggacatt
    47521 accaagcaga tttatttgaa agatgcgagt ctggatattg tgtggaatag tttgtggccg
    47581 ctactggtga taacggccac gacagggtca gcggcgtacg cgatgtttag acgtaaggtg
    47641 atgtaacttc ttatctttcg ccagcaaaga cactaccgcc gggccagcaa ggattgccag
    47701 ccctgcaatc gccagcaaaa agttgttttc cagtacgcgg gacaacagcc acagcacaat
    47761 cagcgctgcg ccaaaatacc aggtcgtggt cagctcttcc agcacgtctg ccacctcacg
    47821 ccagcgttgc tcggggtggc gctgaaacac cagcatcatc actaccgtga cacaatacag
    47881 tgcgcataat cccacgccga cgtgaaccag agtcttactc atccagccca ccaccagcag
    47941 cgccactacc agtaaatgca acataatctg ccagcaactt agacccgttg cgacacgaac
    48001 acgttgttgc cacttcatgg cttctctcct gaagaattta tctctactta ctcagagtac
    48061 cgaactttac ggaaaattcc gtaagaccgc acctttttgt gacgccgact acactatttc
    48121 aatgagataa gaggcaaaga caggaaaatt atgcccgatc aaacacaaca attttcgttc
    48181 aaggtgctca ccatcaatat tcacaaaggc tttaccgcgt ttaaccgacg cttcattttg
    48241 ccggaacttc gcgacgccgt gcgtaccgtc agcgccgata ttgtttgcct gcaggaagtg
    48301 atgggcgcgc acgaagttca tccgctgcat gtggaaaact ggcccgatac ctcgcactac
    48361 gagtttctcg ccgacaccat gtggagcgat ttcgcctacg gtcgcaatgc cgtatacccg
    48421 gaagggcatc acggcaacgc ggttctttca cgttatccca ttgaacatta tgagaatcgc
    48481 gatgtttcgg tcgatggtgc ggaaaagcgc ggcgtgctct actgccgcat tgtgccgccg
    48541 atgaccggga aagcgattca tgtgatgtgc gtacatctgg gcctgcgtga ggcgcaccgt
    48601 caggcgcagc ttgcgatgct cgccgaatgg gtgaatgagc tgccggacgg cgaaccagta
    48661 ttggtggcgg gtgatttcaa cgactggcgg caaaaagcta atcatccgtt aaaaatgcag
    48721 gccgggctgg atgagatatt tacccgcgcc cacggacgcc cggcgcgcac gtttccggtg
    48781 caatttcctc tactacgtct ggacaggatc tatgtcaaaa atgccagcgc cagcgcgcca
    48841 accgcgttgc cgctgcggac atggcgacac ctttctgatc atgccccttt aagtgcggag
    48901 attcatttat gaaatgtagc tggcgcgaag gcaataagat ccagttgctg gaaaacggcg
    48961 agcaatatta tcccgcgatg tttaaggcga ttggcgaggc gcaagaacgc atcattcttg
    49021 aaacgtttat ctggtttgag gatgacgtcg gcaaacagct gcatgcggcg ctactggcag
    49081 cagcgcaacg cggggtgaaa gcggaagtct tgctggatgg ctacggttcg ccggatctca
    49141 gcgatgagtt tgtcaatgaa ctgacggcag ctggcgtggt atttcgctac tacgatcccc
    49201 gtcctcgcct ttttggtatg cgcaccaatg tgtttcgccg gatgcatcgc aaaattgtgg
    49261 tgatcgacgc gcgtatcgcc tttattggcg ggctgaatta ctccgccgag catatgtcca
    49321 gctacggtcc agaggctaaa caggattacg cggtacgcct tgaagggccg attgtcgaag
    49381 atattctcca gtttgagctg gaaaacctgc ctggacagag cgcggcacga cgctggtggc
    49441 gacgtcatca caaagcggaa gagaaccgcc agccgggaga agcgcaggta ttgctggtct
    49501 ggcgcgataa cgaagaacat cgcgatgata ttgaacgtca ctatctgaaa atgctcactc
    49561 aggcgcggcg agaagtcatt atcgccaacg cctacttctt ccccggctat cgatttttac
    49621 acgccttgcg taaagcggca cggcgcgggg tgcggatcaa actgatcatt cagggcgaac
    49681 cggatatgcc gattgtcaga gtcggtgcgc gcttgctgta taactatctg gttaaaggcg
    49741 gcgttcaggt atttgagtac cgccgccgcc cgctccacgg caaagtggca ttgatggacg
    49801 atcactgggc gacagtaggg tccagtaatc tcgatccgct cagtttgtca ctgaatctcg
    49861 aagcaaatgt catcatccac gatcgtcatt ttaaccagac gctgcgcgat aatctgaacg
    49921 gcattattgc cgcagattgt cagcaggtcg atgagaccat gctgccgaaa cgcacctggt
    49981 ggaacctgac caaaagcgtg ctggcgttcc actttttacg ccacttcccg gcgctggttg
    50041 gctggcttcc ggcacataca ccacgtctgg cgcaggttga tccccccgca caaccgacaa
    50101 tggaaacgca ggatcgggta gaaactgaaa acacgggggt aaaaccctga tgagtaaatc
    50161 acacccgcgc tggcgcttag caaagaagat cctcacctgg ctgtttttta tcgcggtgat
    50221 tgtgttgctg gtggtctacg ccaaaaaagt ggactgggaa gaggtctgga aggtcatccg
    50281 cgactacaat cgcgttgcgc tgctcagtgc ggtcgggctg gtggtcgtca gctatctgat
    50341 ttacggctgc tatgacctgc tcgcccgttt ctactgcggt cacaaactgg cgaagcgcca
    50401 ggtgatgctg gtgtcgttta tctgctacgc cttcaacctg acgctcagta cctgggtcgg
    50461 cggcataggt atgcgctatc gtttgtactc gcggctaggg ttaccgggca gcactattac
    50521 gcggattttc tcgctcagta ttaccaccaa ctggctgggc tatattttgc tggccgggat
    50581 tatctttacc gcaggcgtgg tggagctgcc ggatcactgg tatgtcgatc aaaccacgct
    50641 gcgtattctc ggcattggct tattgatgat tatcgcggtt tatttgtggt tttgcgcttt
    50701 cgcgaagcac cgccatatga ccatcaaagg gcaaaagctg gtgctgcctt catggaaatt
    50761 cgccctcgcc cagatgctga tttccagcgt taactggatg gtcatgggag cgataatctg
    50821 gctgttactt ggtcaaagcg tgaactattt ctttgtactg ggcgtgttgc tggttagtag
    50881 tattgccggg gtgattgtgc atattccggc ggggattggt gtgctggaag cggtgtttat
    50941 cgcactactg gctggggagc atacctccaa aggtagcatt atcgccgccc tactcgctta
    51001 ccgtgtgctg tattacttta tcccactgct gctggcgctg atctgttacc tgctactgga
    51061 aagccaggcg aagaagctgc gggcgaaaaa tgaagcggcg atgtgaagtt cggttaagtt
    51121 ctttagtatg tgcatttacg actaatgaaa aaaccgcgta tgcctttgcc agacaagcgt
    51181 tatagctatc gcggcaagta tcccaaagaa gccgataaaa tagtaataga gtgttaatgc
    51241 acctgtaaaa gcgcaactaa ccggactcca caactcttcg ctatggcatt ttcctgccag
    51301 ttcaaggagc ttatttcgtc catgcgagat aatgcctgac cataaaacaa tcattaagca
    51361 acttattacc caaatgggcc gttcactcat taatgcggca ttcctgatga tcaccagggc
    51421 cagtcctgcg gttgtcacca aacccaaagc ggtaatgagc catatgccgt ttaatatcct
    51481 ggcttcgtgc tgaaaatcta atttattgct gctttgcgag tatgattcca ttcttttagt
    51541 tatccttaga aaaaatatta atattcacca tcaataaatt taaagccacg catacaaaat
    51601 acttttgtat ttatggatac ggaaaacact tccgctcggc cataaggcat attttatact
    51661 tggtacttgt taagtgtcgt tttttcgtaa gaaaaaaatc ccgacacatg gccgggatat
    51721 tgatggtgaa aaagaattaa cggcggttgc cgaagatccg caacaacatc aggaacaagt
    51781 tgatgaagtc cagatacaag gttaacgcgc caagaatgga atatttgcgc aggttcgacg
    51841 tgtcgcgggt atcaatctgc tcacccatat ttttcaattt ctgcgtgtca tacgccgtca
    51901 atccgacaaa gacaatcacg ccaatgtagg taactgccca catcaatgct tcgcttttca
    51961 gccagaagtt gaccagcgat gccagcacaa tgccgattaa cgccataaac agcatattgc
    52021 caaagccgct tagatcgcgc ttcgtggtgt aaccgtacag gctcattgcg ccaaacattc
    52081 cggcagtaac gacgaaagta ctggcgatag aagcagcggt ataaacaatg aaaatactgg
    52141 aaagcgtaag acccgtcagc gccgaataaa gcataaagag catcgtcgtt acacctgcgc
    52201 tcagcttttg aatcatcgct gataacacaa taaccaatgc taattgcgcg atgatcagac
    52261 cgattaaaaa gacacggtta gtgaacaaca gctccatcac agccgcggaa ttagccgcat
    52321 accaggcaac aaatgcggtc agcaacaagc caacggtcat ccagccatag acttgcgcca
    52381 tataagtttg caagccagcc cggggttgta caattgaatc agaacgtggg aatctgtcca
    52441 tgacgatctc ctgaagatat aaggaatatc ttaaggatac tgcaaaatga tgaggctgtg
    52501 catcgacgca gcgtaaacgc atgtactgag cggtgaaatt gccggacgca gcggtgcctt
    52561 atccggctaa caaaaaacta ccagcgtttt gccgcctgct gatcgctctc ccgagcttca
    52621 acccagcggt cgccttccgg cgtggcttca cgcttccaga acggtgcgcg ggttttgaga
    52681 taatccataa taaactgccc ggcttcaaac gcactgctgc gatgcgcact ggtgacaccg
    52741 acaaaaacga tttcatctcc cggccataat tccccgatgc ggtgaatcac cgtgacgcgc
    52801 cccagcggcc agcggttacg cgcttcatcg acaatttctg ccagtgcttt ttcagtcatc
    52861 cccggatagt gttcgagggt taatgctttg acgctgtcgc caaggttatg gttgcgcacc
    52921 ttaccggtaa aggtgactac cgcaccgtct tcatcacgct ccgccagcca cggatactct
    52981 tctcctacgc tgaatggctg cgggccgaca acaattttgg tttctgccat cttaacctcc
    53041 ggttaccggc gggaagaaag ccacttcgtc gccgtcagtc agcggatggt caagactcac
    53101 cagcgtctgg ttgacggcag ccagcaattt gccatcttcc agcgccagcg cccagcgatc
    53161 gctctgcgca gccagatgtt ggcgtaacgc ttcaacggtt gggaaatccg cagccacttc
    53221 ggttgcatct gttcccacca actcgcgcac ctgggcgaaa aaaagaactt taatcatccg
    53281 cttccacctt aaagtcaccc gacttgccgc cgctcttcgc cagcaaacgt accggaccaa
    53341 ttaccatatc tttttgcacc gctttgcaca tgtcataaat ggtcagcgcc gccacggagg
    53401 ccgcggttaa cgcttccatt tcgacaccgg ttttcccggt caggcggcat aaggtttcta
    53461 tacgtacccg attgtgctcc ggctcggcct gtaaattgac ttcaactttg ctgagcatca
    53521 gcgggtgaca cagcgggatc agatcccagg tgcgttttgc cgcctgaata ccggcgatac
    53581 gcgcagtggc aaatacgtcg cctttgtggt ggcgaccatc aataatcatc gccagcgtct
    53641 cgctgcgcat ggtgacaaag gcttccgccc gcgcttcacg cacggtttcc gctttggcgg
    53701 agacatccac catgtgtgct tcgccagcgg cgttgatatg ggtcagttgc gacatactta
    53761 tttcttcaaa tgtggatgga aattgcatgg acgtgtacgg gcatcgagct gcggcgcgat
    53821 gatattttcc cacgcggtgc ggcacgcttt ggtcgaaccc ggcatggcga aaatcagcgt
    53881 tttgttagcg acgcccgcta ccgcacgaga ttgcaacgtt gaggtgccaa tctcttcaaa
    53941 cgacaacatc cggaacactt caccaaaacc ttcaacttca cggtcgaaca acggcagcag
    54001 cgcttcggga gcctgatcac cttccgtcag gccagtacca cccgtaatca ataccacttg
    54061 tacatcgtcg ctggcgatcc acgcagatac ctgagcgcga atagcgtagc ggttttcttt
    54121 cacaatggct ttatcgacaa cgtgatggcc cgcttcctgc gccgaatcgc gcagatagtg
    54181 accggaggta tcgtcttctt caccacgacg attagaaacc gtaagaatag caatacgggt
    54241 cgggataaat tcagtgctta cctgactcat ctgatctctc cttttgacgt tttagccgcc
    54301 aatgtacgat aagttttgcg taataccggt gttgttttga tgcaggaaat gggtctgttt
    54361 cttctcccgc agcgccgctg aaatacgcgc ttccagcgcc tgttgctggg tatcgtcttc
    54421 cagcagatcg cgcaggttaa cgccgccttc accaaacagg cagagatgga gtttaccaat
    54481 ggaggaaacg cgcaggcggt tgcaagtggc gcagaagtct ttttcatacg gcatgataag
    54541 gccaatctct ccggcgtaat ccggatgaca aaagacttgc gcgggaccgt cgctgcgttg
    54601 acgtaattgg tggatccagc cgcgacgcag tagctcgtca cgcagaacct gaccagagat
    54661 gtgatgctta cggaagagct cgctgccctc acccgtttcc atcaattcga tgaaacgcag
    54721 ctggataggg cgatgctgga tccagttcag aaaggtgtcg agctgatgat gattaacatc
    54781 acgcatcagc acggtattga ctttgacctt ctcaaaaccg gcctcaaatg cagcatcgat
    54841 ccctgccatg acctggttga atttatcctg cccggtaatg gcatgaaact ggcgagcgtc
    54901 aaggctatca acgctgacgt tgatgccagt aagtcccgca tcgcgccagt tcgccacatc
    54961 gcgttccaga cggtaaccat tggtggtgac cgcaatctgg cggatagcgt cgttttcccg
    55021 cacagcggcg atgatatcgg taaagtcgcg gcgtaaagac ggctcaccgc ctgtcaggcg
    55081 cactttttcg gtgcccagac tggcaaaggc gcgcgtaacc cggcgaattt catcgacggt
    55141 aagaaagcct ttattggtga cgccgctcgg tttgtagcca tccggcaggc agtaggtgca
    55201 acgaaagtta cacacatcag taatcgacag gcgcaagtag taaaacttac gcgcaaatgc
    55261 atcagtcagt tgtgaagcca tgtacacctt tccagatacg ggaggcgaag tcatttcttc
    55321 cttcgccctg gtggcaatct ttacgagcat tgtcacggcc aaagcaccgt atcagttgac
    55381 ccaggtgcag aggctagagt gtttagtggt taagccgata ctagcgtgta aatgagattt
    55441 ttaccatcca cactttcgct atgtaatcat gtatatagcg tcatgatcgt gctatttcgc
    55501 tacagaataa gggaaaatgg cggttttact ttgatataca tcatcaggaa ggcatagcgt
    55561 acatcgtcct ttagaggtag aagaaaatag tttgtatcct caatattggc aggttaattg
    55621 ctgtttcccc gcaatttgcg ctactgtagc gcggataatc tgactccagg aaactatatg
    55681 cgcaatcgta cgctggctga ccttgatcgt gtcgttgctc tcggcggagg gcatggactg
    55741 ggacgcgttc tctcatcact ttcgtctttg ggttctcgtt taacgggtat cgtcaccacc
    55801 acggataatg gcggatcgac agggcgtatt cgccgctcag aaggcggtat tgcctggggc
    55861 gatatgcgta actgcctcaa ccagctgata actgaaccca gcgtcgcttc cgcaatgttt
    55921 gaatatcgtt tcggcggtaa tggcgaactt tccgggcaca accttggaaa tctgatgctg
    55981 aaggcactgg atcaccttag cgtgcgacct cttgaagcaa tcaatctgat tcgcaatctg
    56041 ctgaaagtgg acgcacattt gattccgatg tcggagcatc cggtggatct gatggcgatt
    56101 gacgatcagg ggcatgaagt ttacggcgag gtcaatatcg accagttaac tacgccgatt
    56161 caggagttac tgttaacgcc gaatgtaccc gcaacgcgtg aggctgttca cgctatcagt
    56221 gaagcggatc tcatcattat tgggcctggc agtttttaca ccagcctgat gccaattctg
    56281 ctgctgaagg aaatcgcaca ggcattacgc cgcacgccag cgccgatggt ctatataggc
    56341 aatctggggc gtgagttgag tttacctgcg gctaatttga agctggaaag caagctggca
    56401 attatggagc agtatgtcgg taaaaaggtc attgatgcgg tcatcgtcgg gccaaaagtg
    56461 gatgtctcgg cggtgaaaga gcggattgtg attcaggagg tactggaggc cagcgatatt
    56521 ccgtatcgtc atgaccgcca gttgttacat agcgcgctgg aaaaggcatt acaggcttta
    56581 ggttaacgga aagccgaagg aaaattccgg cttcctattg aagacaaagt gcgcgttgtt
    56641 tatgccggat gcggcgtgaa agccttatcc ggcctacaaa ccgcgctaat tcaatgtatt
    56701 gcagagatca tgtaggcctg ataagcgtag cacatcaggc aattttgctt ttgtcatctg
    56761 tcttcttcgt tatcctgtca cgaagccgca atgaacaact cacgcagctg atgcaactgg
    56821 tcacgaattt gtgccgcttc ttcgaactcc agattctgcg cgtgttgcat catcaaccct
    56881 tccagttcat ggattttctg ctgcaacgct ttaggcgaca tatccatcgg cacattatcc
    56941 ggctcaacaa tcggtcgcga ttttcctctg cccttcgctt tggttttggc aatgttctgc
    57001 cccagcgcca ggatatcgac cactttcttg ttcaagcctt gcggcgtaat gccgtgttcc
    57061 tcgttgtact tctgctgttt ctcgcggcga cgttcggttt cgccaatcgc tttcgccatt
    57121 gatggggtga tcttatcgcc gtagagaatc gctttaccgt taacgttacg tgccgcacga
    57181 ccaatggtct ggatcaacga acgttcggaa cgcaggaagc cttctttgtc agcgtcgagg
    57241 atcgccacca gcgaaacttc cggcatatcc agaccttcgc gcagtaagtt gatccctacc
    57301 aatacgtcga actcacccag acgcaagtcg cggataatct ccatacgttc gacggtgtcg
    57361 atatctgagt gaagataacg cacgcgctca ccgtgttctt cgagatattc agtgagatct
    57421 tccgccatcc gcttggtcag agttgtaacc agtacgcgtt cgttaattgc cgctcgctga
    57481 cgaatctccg aaagaagatc atccacctgt gttgccactg gccgcacttc gataatcggg
    57541 tcgagtaagc ctgttggacg caccacctga tccaccacat cgccaccgga tttttccagc
    57601 tcgtaattac ccggcgtcgc cgaaacatag atggtttgcg gcgctaatgc ttcgaactct
    57661 tcaaatttca gcggacggtt atccagcgct gatggcaggc ggaagccgta ctccaccagt
    57721 gtctctttac gcgcccggtc accgcgatac atgccgccaa tttgtggaat ggtgacgtga
    57781 gattcatcga ccaccagcag cccatcagca ggcaggtaat caaacagcgt cggcggtggc
    57841 tcacccggtc cacgaccgga gaggaagcgc gagtagttct caatccccga acagtagcct
    57901 agctcgttca tcatctccag atcaaactgg gtacgctggg tcagccgctg ctcttccagc
    57961 agtttgttgt tttccaatag cactttacgt ctggcggcca gctcttcttt gatctcctcc
    58021 atcgcctgga cgatgcgctc gcgtggcgtg acgtagtgcg ttttcgggta gatggtaaaa
    58081 cgtggaatag tggaaacaat ctgcccggtc agcgggtcaa ataacgacaa tcgttccact
    58141 tcctcgtcaa acagctccac gcgaagtgca atgtcatccg attctgccgg gaagatatca
    58201 atcacctcgc cacgaacacg gaaagtacca cgctggaagg cttgatcatt acgagcatat
    58261 tgcagctccg ccagtcggcg caggatcgcg cgctgatcga taatcatacc gaccgtgaga
    58321 tggagcatca tcttgagata taaatcagga tcgcccagac cataaatcgc ggaaacagac
    58381 gccaccacaa ccacatcacg ccgctccagc atcgctttgg tggcggacaa acgcatctgt
    58441 tcgatatgtt cgtttaccga ggcatctttc tcaatgaagg tgtcggaact cggtacatag
    58501 gcctccggct gatagtagtc gtagtaggag acgaaatatt ccaccgcgtt ttccgggaag
    58561 aattctttca tttcgccata cagctgggcc gccagcgttt tgttgggcgc aagtaccatg
    58621 gttgggcgct gaaggtcagc aatgacattg gcaatggtga aggttttccc ggagccagtc
    58681 acgccaagta acgtctggtg cgccaggcca tcttccagcc cctcttcgag acgtcgaatc
    58741 gcctccggct gatcgccaga aggtttaaaa gcggaattca gtttgaacgg tttactcatg
    58801 agtcgctacc tgaaggagtt gggcgggcag gtatgtaatt ttactcgtcg tacttaatta
    58861 tgccaacaaa ttatactgga taaaaaaaca gttcatcacc ataatatttc tgttacagcg
    58921 taaactccgc tgtcaatcat gagcaaaatt tactctgcgg cgagataaaa ctccggcctt
    58981 accgggttat ccccaaagca acggcttttt taacatttgt caagatgatt gatgacagtt
    59041 ttatgg
//
LOCUS       DAZKGF010000009        54483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZKGF010000009 DAZKGF010000000
VERSION     DAZKGF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.009"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            203..1297
                     /gene="gcvT"
                     /locus_tag="ACHOQV_000559"
     CDS             203..1297
                     /gene="gcvT"
                     /locus_tag="ACHOQV_000559"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFP9628983.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1321..1710
                     /gene="gcvH"
                     /locus_tag="ACHOQV_000560"
     CDS             1321..1710
                     /gene="gcvH"
                     /locus_tag="ACHOQV_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFP9628984.1"
                     /translation="MSNVPAELKYSKEHEWLRKEAGGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1829..4702
                     /gene="gcvP"
                     /locus_tag="ACHOQV_000561"
     CDS             1829..4702
                     /gene="gcvP"
                     /locus_tag="ACHOQV_000561"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFP9628985.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4866..6299)
                     /gene="bglA"
                     /locus_tag="ACHOQV_000562"
     CDS             complement(4866..6299)
                     /gene="bglA"
                     /locus_tag="ACHOQV_000562"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFP9628986.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLHEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            6344..6655
                     /gene="yqfB"
                     /locus_tag="ACHOQV_000563"
     CDS             6344..6655
                     /gene="yqfB"
                     /locus_tag="ACHOQV_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFP9628987.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTDLKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            6819..7478
                     /gene="yqfA"
                     /locus_tag="ACHOQV_000564"
     CDS             6819..7478
                     /gene="yqfA"
                     /locus_tag="ACHOQV_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP9628988.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     IKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(7555..8535)
                     /gene="ygfZ"
                     /locus_tag="ACHOQV_000565"
     CDS             complement(7555..8535)
                     /gene="ygfZ"
                     /locus_tag="ACHOQV_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFP9628989.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            8788..9054
                     /gene="sdhE"
                     /locus_tag="ACHOQV_000566"
     CDS             8788..9054
                     /gene="sdhE"
                     /locus_tag="ACHOQV_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFP9628990.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9035..9442
                     /gene="ygfX"
                     /locus_tag="ACHOQV_000567"
     CDS             9035..9442
                     /gene="ygfX"
                     /locus_tag="ACHOQV_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFP9628991.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9482..10003)
                     /gene="fldB"
                     /locus_tag="ACHOQV_000568"
     CDS             complement(9482..10003)
                     /gene="fldB"
                     /locus_tag="ACHOQV_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFP9628992.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10115..11011
                     /gene="xerD"
                     /locus_tag="ACHOQV_000569"
     CDS             10115..11011
                     /gene="xerD"
                     /locus_tag="ACHOQV_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP9628993.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11036..11746
                     /gene="dsbC"
                     /locus_tag="ACHOQV_000570"
     CDS             11036..11746
                     /gene="dsbC"
                     /locus_tag="ACHOQV_000570"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFP9628994.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            11752..13485
                     /gene="recJ"
                     /locus_tag="ACHOQV_000571"
     CDS             11752..13485
                     /gene="recJ"
                     /locus_tag="ACHOQV_000571"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP9628995.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHSGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGVA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAL
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLTYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            13576..14674
                     /gene="prfB"
                     /locus_tag="ACHOQV_000572"
     CDS             join(13576..13650,13652..14674)
                     /gene="prfB"
                     /locus_tag="ACHOQV_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP9628996.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            14684..16201
                     /gene="lysS"
                     /locus_tag="ACHOQV_000573"
     CDS             14684..16201
                     /gene="lysS"
                     /locus_tag="ACHOQV_000573"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9628997.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(16244..16792)
                     /gene="idi"
                     /locus_tag="ACHOQV_000574"
     CDS             complement(16244..16792)
                     /gene="idi"
                     /locus_tag="ACHOQV_000574"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP9628998.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     YYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            16847..16918
                     /locus_tag="ACHOQV_000575"
     CDS             16847..16918
                     /locus_tag="ACHOQV_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9628999.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(16915..17040)
                     /gene="yqfG"
                     /locus_tag="ACHOQV_000576"
     CDS             complement(16915..17040)
                     /gene="yqfG"
                     /locus_tag="ACHOQV_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFP9629000.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17042..18490)
                     /gene="uacT"
                     /locus_tag="ACHOQV_000577"
     CDS             complement(17042..18490)
                     /gene="uacT"
                     /locus_tag="ACHOQV_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFP9629001.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            18925..20844
                     /gene="ygfT"
                     /locus_tag="ACHOQV_000578"
     CDS             18925..20844
                     /gene="ygfT"
                     /locus_tag="ACHOQV_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFP9629002.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAMAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQPDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAACVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            20844..21332
                     /gene="ygfS"
                     /locus_tag="ACHOQV_000579"
     CDS             20844..21332
                     /gene="ygfS"
                     /locus_tag="ACHOQV_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9629003.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVSACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(21368..22735)
                     /gene="ghxQ"
                     /locus_tag="ACHOQV_000580"
                     /gene_synonym="ygfQ"
     CDS             complement(21368..22735)
                     /gene="ghxQ"
                     /locus_tag="ACHOQV_000580"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFP9629004.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(22771..24087)
                     /gene="guaD"
                     /locus_tag="ACHOQV_000581"
     CDS             complement(22771..24087)
                     /gene="guaD"
                     /locus_tag="ACHOQV_000581"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFP9629005.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(24105..25505)
                     /gene="xanQ"
                     /locus_tag="ACHOQV_000582"
     CDS             complement(24105..25505)
                     /gene="xanQ"
                     /locus_tag="ACHOQV_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFP9629006.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGATLQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(25670..28540)
                     /gene="xdhD"
                     /locus_tag="ACHOQV_000583"
     CDS             complement(25670..28540)
                     /gene="xdhD"
                     /locus_tag="ACHOQV_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFP9629007.1"
                     /translation="MIIHFTLNGTPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEEDNC
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKRDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(28537..29316)
                     /gene="ygfM"
                     /locus_tag="ACHOQV_000584"
     CDS             complement(28537..29316)
                     /gene="ygfM"
                     /locus_tag="ACHOQV_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFP9629008.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQTLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEEYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVEKQNLEGDALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(29367..30695)
                     /gene="ssnA"
                     /locus_tag="ACHOQV_000585"
     CDS             complement(29367..30695)
                     /gene="ssnA"
                     /locus_tag="ACHOQV_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFP9629009.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKGGCTSVIDHHASPAYIDGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDDAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSHSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLCDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(30698..33796)
                     /gene="ygfK"
                     /locus_tag="ACHOQV_000586"
     CDS             complement(30698..33796)
                     /gene="ygfK"
                     /locus_tag="ACHOQV_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFP9629010.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSCKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPPELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTELKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVDSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDNEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(34118..34696)
                     /gene="mocA"
                     /locus_tag="ACHOQV_000587"
     CDS             complement(34118..34696)
                     /gene="mocA"
                     /locus_tag="ACHOQV_000587"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFP9629011.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKIMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            34800..35570
                     /gene="yqeC"
                     /locus_tag="ACHOQV_000588"
     CDS             34800..35570
                     /gene="yqeC"
                     /locus_tag="ACHOQV_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFP9629012.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRSECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            35618..37243
                     /gene="yqeB"
                     /locus_tag="ACHOQV_000589"
     CDS             35618..37243
                     /gene="yqeB"
                     /locus_tag="ACHOQV_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFP9629013.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTECGFIPVIVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(37284..38216)
                     /gene="arcC"
                     /locus_tag="ACHOQV_000590"
     CDS             complement(37284..38216)
                     /gene="arcC"
                     /locus_tag="ACHOQV_000590"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9629014.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(38264..39649)
                     /gene="hyuA"
                     /locus_tag="ACHOQV_000591"
     CDS             complement(38264..39649)
                     /gene="hyuA"
                     /locus_tag="ACHOQV_000591"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFP9629015.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGSNISPQVPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(39702..40913)
                     /gene="ygeY"
                     /locus_tag="ACHOQV_000592"
     CDS             complement(39702..40913)
                     /gene="ygeY"
                     /locus_tag="ACHOQV_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFP9629016.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(40971..42167)
                     /gene="dpaL"
                     /locus_tag="ACHOQV_000593"
     CDS             complement(40971..42167)
                     /gene="dpaL"
                     /locus_tag="ACHOQV_000593"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFP9629017.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(42225..43412)
                     /gene="ygeW"
                     /locus_tag="ACHOQV_000594"
     CDS             complement(42225..43412)
                     /gene="ygeW"
                     /locus_tag="ACHOQV_000594"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFP9629018.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            43891..45669
                     /gene="ygeV"
                     /locus_tag="ACHOQV_000595"
     CDS             43891..45669
                     /gene="ygeV"
                     /locus_tag="ACHOQV_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9629019.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDENRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFISEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLALDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(45709..46188)
                     /gene="xdhC"
                     /locus_tag="ACHOQV_000596"
     CDS             complement(45709..46188)
                     /gene="xdhC"
                     /locus_tag="ACHOQV_000596"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFP9629020.1"
                     /translation="MNHSETITIECTINGIPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITGIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(46185..47063)
                     /gene="xdhB"
                     /locus_tag="ACHOQV_000597"
     CDS             complement(46185..47063)
                     /gene="xdhB"
                     /locus_tag="ACHOQV_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFP9629021.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCQLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(47074..49371)
                     /gene="xdhA"
                     /locus_tag="ACHOQV_000598"
     CDS             complement(47074..49371)
                     /gene="xdhA"
                     /locus_tag="ACHOQV_000598"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFP9629022.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAISINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALADDAAPIHNGGN
                     LLKQSSMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALNIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            49787..50539
                     /gene="actS"
                     /locus_tag="ACHOQV_000599"
     CDS             49787..50539
                     /gene="actS"
                     /locus_tag="ACHOQV_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFP9629023.1"
                     /translation="MSAGRLNKKSLGIVMLLSIGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKNSSTRKSTAKSTTK
                     TASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYAA
                     GAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGSTD
                     AASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            50618..50691
                     /locus_tag="ACHOQV_000600"
     tRNA            50618..50691
                     /locus_tag="ACHOQV_000600"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50650..50652,aa:Gly,seq:ccc)
     gene            50856..52070
                     /locus_tag="ACHOQV_000601"
     CDS             50856..52070
                     /locus_tag="ACHOQV_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9629024.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARAARDEAKRLIAGGVNPSDVRKAHKREQASKFGNTFEA
                     IAREWYEKRLDRWSQSYAAEMIDTFEKDVFPYIGSKPIAEIKPMELLAVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEQG
                     GNVVHGKFGKCG"
     gene            52198..52893
                     /locus_tag="ACHOQV_000602"
     CDS             52198..52893
                     /locus_tag="ACHOQV_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629025.1"
                     /translation="MPVVNDFPDLSHWFTVQEFTITQAALLLSGIDPLDYPTLEMVKE
                     EKHERWRMAYGFAYGMVTAIRRGVLTPVLCVAYDWGDMRRLTPAEMMDRSNNISMDGT
                     IITRYSLQEWVKNEKVDYVRPSRSISDKEIKPSNQSVIIDAESSVINSEETVLLTYGH
                     KSEGLEYVEDAIRELWATYDPDNQQTAPTRNDVVEYLKGRGAGQNMAEAVNLILRPTG
                     LQQVGRRKRKTNS"
     gene            53141..53338
                     /locus_tag="ACHOQV_000603"
     CDS             53141..53338
                     /locus_tag="ACHOQV_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9629026.1"
                     /translation="MSNNLLRLSDVMRRTGYGRAWIYRLINQGRFPKPVKIGPRSVAF
                     IESEVDEWINQRIEASRNNAA"
     gene            53419..54195
                     /locus_tag="ACHOQV_000604"
     CDS             53419..54195
                     /locus_tag="ACHOQV_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP9629027.1"
                     /translation="MPVQGQGFVQPEELSQQYFAAIIPVISGNEIIADGINKNALTAP
                     EVFPSGGEPVTTSQAIARFFRKDHDKVVNRIENLCCSAEFLTRNFRRVKFEHKGRVLK
                     AYEVTKDGFVFLTMGFTGKKAAAFKEAYIAQFNRMANWIAERCEAKAEMRSMGDAMKG
                     YIERTGDTQHGHAYNNECTFIARLVLGMHPPVWAKKNGIPSKKVRDHMTADQLALLAY
                     LESRNCALLDMDMPTPERKERLTELAQRWLVRRLGEREHA"
     gene            54188..>54483
                     /locus_tag="ACHOQV_000605"
     CDS             54188..>54483
                     /locus_tag="ACHOQV_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ash family protein"
                     /protein_id="HFP9629028.1"
                     /translation="MPDILKPAVRYIDTADKFALLNINHADYMRHIVSHFTAERMKHV
                     KQYLEEGLSNYRAGGPVRREYPHDAGMAGKTKATTKGSPVEIKEIKRIIHNAIS"
BASE COUNT        13426 a        13507 c        14257 g        13293 t
ORIGIN      
        1 ttctgttgcg atttttgcat tttttaacca taagctaatg tgatgatcaa ttttacctta
       61 tggttaacag tctgtttcgg tggtaagttc aggcaaaaga gaacgattgc gttggggacc
      121 gggagtggct ccgatgctgg gtttcgtggt gataatttcg ccatggaaaa gttgtcagcc
      181 ccgcttattc aatgaggaca agatggcaca acagactcct ttgtacgaac aacacacgct
      241 ttgcggcgct cgcatggtgg atttccacgg ctggatgatg ccgctgcatt acggttcgca
      301 aatcgacgaa catcatgcgg tacgtaccga tgccggaatg tttgatgtgt cacatatgac
      361 catcgtcgat cttcgcggca gccgcactcg ggagtttctg cgttatctgc tggcgaacga
      421 tgtggcgaaa ctcaccaaaa gcggcaaagc cctttactcg gggatgttga atgcctctgg
      481 cggtgtgata gatgacctca tcgtctacta ctttactgaa gatttcttcc gcctcgttgt
      541 taactccgcc acccgcgaaa aagacctctc ctggattacc caacacgctg aacctttcgg
      601 catcgaaatt accgttcgtg atgacctttc catgatcgcc gtacaagggc cgaatgcgca
      661 ggcaaaagct gccacactgt ttaatgacgc ccagcgtcag gcggtggaag ggatgaaacc
      721 gttctttggc gtgcaggcgg gcgatctgtt tattgccacc actggttata ccggtgaagc
      781 gggctatgaa attgcgctgc ccaatgaaaa agcggcggat ttctggcgcg cgctggtgga
      841 agcgggcgtt aagccatgcg gcctgggcgc gcgtgacacg ctgcgtctgg aagcgggtat
      901 gaatctttat agtcaggaga tggacgaaac catctctcct ttagccgcca atatgggctg
      961 gactatcgcc tgggaaccgg cagatcgtga cttcatcggt cgtgaagccc tggaagtgca
     1021 gcgtgagcat ggcacagaaa aactggttgg tctggtgatg accgaaaaag gcgtgctgcg
     1081 taatgaactg ccggtacgct ttaccgatgc gcagggcaac cagcatgaag gcattatcac
     1141 cagcggtact ttctccccga cgctgggtta cagcattgcg ctggcgcgcg tgccggaagg
     1201 tattggtgaa acagcgattg tacaaattcg caaccgtgaa atgccggtta aagtgacgaa
     1261 acctgttttt gtgcgtaacg gtaaagccgt cgcgtgattt acttttttgg agattgattg
     1321 atgagcaacg taccagcaga actgaaatac agcaaagaac acgaatggct gcgtaaagaa
     1381 gccggcggca cttacaccgt tggtatcacc gaacatgccc aggagctgtt aggcgatatg
     1441 gtgtttgttg acctgccgga agtgggcgca acggttagcg cgggcgatga ctgcgcggtt
     1501 gccgaatcag taaaagcggc gtcagacatt tatgcgccag taagcggtga aatcgtggcg
     1561 gtaaacgacg cactgagcga ttccccggaa ctggtgaaca gcgaaccgta tgcaggcggc
     1621 tggatcttta aaatcaaagc cagcgatgaa agcgaactgg aatcactgct ggatgcgacc
     1681 gcatacgaag cattgttaga agacgagtaa cggctttatt cctcttctgc gggagaggat
     1741 cagggtgagg aacttttacg cctcaccctc actctcttcg caaggagaga ggttcacaat
     1801 tcactgcacg tttcaggaac catcgctcat gacacagacg ttaagccagc ttgaaaacag
     1861 cggcgctttt attgaacgcc atatcggacc ggacgccgcg caacagcaag aaatgctgaa
     1921 tgccgttggc gcacaatcgt taaacgcgct gaccggccag attgtgccga aagatattca
     1981 gcttgcgacc ccgccgcagg ttggcgcacc ggcgaccgaa tacgccgcgc tggcggaact
     2041 caaggctatt gccagtcgca ataaacgctt cacgtcttac atcggcatgg gttacaccgc
     2101 cgtgcagcta ccgccggtta ttctgcgtaa catgctggaa aatccgggct ggtataccgc
     2161 gtatactccg tatcaacctg aagtctccca gggccgcctt gaagcactgc tcaacttcca
     2221 gcaggtaacg ctggatttga ccgggctgga tatggcctct gcctctcttc tggacgaggc
     2281 cactgctgcc gccgaagcaa tggcgatggc gaaacgcgtc agcaaactga aaaatgccaa
     2341 ccgcttcttc gttgcttctg acgttcatcc gcaaacgctc gatgtggtcc gtactcgtgc
     2401 tgaaaccttt ggttttgaag tgattgtcga tgacgcgcaa aaagtgctcg accatcagga
     2461 cgtcttcggc gtgctgttac agcaggttgg cacaaccggt gaaatccacg actacactgc
     2521 gctcatcagc gaactgaaat cacgcaaaat tgtggtcagt gtcgccgccg atataatggc
     2581 gctggtgctg ttaactgcgc cgggtaaaca gggcgcggat attgtctttg gttcggcgca
     2641 acgcttcggt gtgccgatgg gctacggtgg cccacacgca gcattctttg cggcgaaaga
     2701 tgaatacaaa cgctcaatgc cgggccgtat tatcggtgta tcgaaagatg cagctggcaa
     2761 taccgctctg cgcatggcga tgcagactcg cgagcaacat attcgccgtg agaaagcgaa
     2821 ctccaacatt tgtacttccc aggtactgct ggcaaacatc gccagcctgt atgccgttta
     2881 tcacggcccg gttggcctga aacgtatcgc taaccgcatc caccgtctga ccgatatcct
     2941 ggcggcgggc ctgcaacaaa aaggtctgaa actgcgtcat gcgcactatt tcgacacttt
     3001 gtgtgtggaa gtggcagaca aggcgggcgt actggcgcgt gccgaagcgg ctgaaatcaa
     3061 cctgcgtagc gatatcctga acgcggttgg gatcaccctt gatgaaacca ccacgcgtga
     3121 aaacgtgatg cagcttttca gcgtactgct gggggataac cacggcctgg acatcgacac
     3181 gctggacaaa gacgtggctc acgacagccg ctctattcag cctgcgatgc tgcgcgacga
     3241 cgaaatcctc acccatccgg tgtttaatcg ctaccacagc gaaaccgaaa tgatgcgcta
     3301 tatgcactcg ctggagcgta aagatctggc gctgaatcag gcgatgatcc cgctgggttc
     3361 ctgcaccatg aaactgaacg ccgccgccga gatgatccca atcacctggc cggaatttgc
     3421 cgaactgcac ccgttctgcc cgccggagca ggccgaaggt tatcagcaga tgattgcgca
     3481 gctggctgac tggctggtga aactgaccgg ttacgacgcc gtttgtatgc agccgaactc
     3541 tggcgcacag ggcgaatacg cgggcctgct ggcgattcgt cattatcatg aaagccgcaa
     3601 cgaagggcat cgtgatatct gcctgatccc ggcttctgcg cacggaacta accccgcttc
     3661 tgcacatatg gcaggaatgc aggtggtggt tgtggcgtgt gataaaaacg gcaacatcga
     3721 tctgactgat ctgcgcgcga aagcggaaca ggcgggcgat aacctctcct gtatcatggt
     3781 gacctatcct tccacccacg gcgtgtatga agaaacgatc cgtgaagtgt gtgaagtcgt
     3841 gcatcagttc ggcggtcagg tttaccttga tggcgcgaac atgaacgccc aggtcggcat
     3901 cacctcaccg ggctttattg gcgcggacgt ctcgcacctt aacctgcata aaactttctg
     3961 cattccgcac ggtggcggcg gccctggtat gggaccgatc ggcgtgaaag cgcatctggc
     4021 accgtttgtg ccaggtcata gcgtggtaca aattgaaggc atgttaaccc gtcagggcgc
     4081 ggtttctgcg gcaccgttcg gtagcgcatc cattctgcca atcagctgga tgtacatccg
     4141 catgatgggc gcagaagggc tgaaaaaagc aagccaggtg gcaattctta acgccaacta
     4201 tattgccagc cgcctgcagg atgccttccc ggtgctgtat accggtcgcg acggtcgcgt
     4261 ggcgcacgaa tgtattctcg atattcgccc gctgaaagaa gaaaccggca tcagcgaact
     4321 ggatattgcc aagcgtctga tcgactacgg tttccacgcg ccgaccatgt cgttcccggt
     4381 ggcgggtacg ctgatggttg aaccgactga atctgaaagc aaagtggaac tggatcgctt
     4441 tatcgacgcg atgctggcta tccgcgcaga aattgaccag gtgaaagccg gtgtctggcc
     4501 gctggaagat aacccgctgg tgaacgcgcc gcacattcag agcgaactgg tcgccgagtg
     4561 ggcgcatccg tacagccgtg aagttgcggt attcccggca ggcgtggcag acaaatactg
     4621 gccgacagtg aaacgtctgg atgatgttta cggcgaccgt aacctgttct gctcctgcgt
     4681 accgattagc gaataccagt aattcactga ttcgactatc ttctaaaggc gcttcggcgc
     4741 ctttttagct tgttaataaa gtgcgctttg tgtgtcggat gaagcgtaaa tataaactcc
     4801 tggagttcaa tatatgacag gagcgccagg cctgatgagc ggggcgcatc aggcacatcg
     4861 ggtaattaaa gcttctcgcc gttgctggca atcacttctt tgtaccagtt aaagctcttc
     4921 ttacgtgaac gcgacatatc gccagtaccg tcgtcatgtt tattcacata gataaagccg
     4981 tagcgtttgc tgtactgccc ggtagtgaac gacacgcaat cgatgcagcc ccacggtgtg
     5041 tagcccatca gatccacgcc atcgtaagtc accgcttttt tcatctcttc aatatgggcg
     5101 cgcaggtagt caatgcggta gtcatcgttg atgctgccat cttcttccac tttgtcataa
     5161 gcgccaaaac cgttttcgac aataaacagc ggcttctgat aacgctcata cagttcgcaa
     5221 agtgcatagc gcagacccac aggatcaatc tgccagcccc agtcagacgc tttaacatac
     5281 gggtttggta cgctgccttc aaaaccagag atcgcatcgc cggtgccgcc ttcggccttc
     5341 accgcgttgg tcatgtaata gctgaaaccg agataatcgc aggtgccttc gcgcagcaca
     5401 tcgagatcgc cgtcttccat tttgatgtta aatccgcgac gctcccactc gtgcaacaca
     5461 taggacgggt aatagccacg cagttgcaca tcggtaaaga cgtagcgttc gcgcatcgac
     5521 tcctgggcga acatcacatc gtccggatta caggagtaag ggtaaagcgg caccatcgcc
     5581 agcatacagc cgactttcat ttccgggtta atgcgacgcg cggctttcac cgccagggcg
     5641 ctggcgacaa actggtgatg cagcacctgg tacatcgtct cttccgggtt ttcatgctcg
     5701 gtatacacca cgccggagca gcagtaaccg aacagcggtg cacgccagtt acgctggttg
     5761 ttaatttcgt tgaaggtcat ccagtatttg actttatgct tatagcgttc aaataccact
     5821 tctgcgaaac ggacaaagaa atcaaccact ttacggttgg tccagctacc gtattgctgc
     5881 accagatgca gcggcatttc aaagtgggag agggtgatca ccggttcgat gttgtatttc
     5941 agcagttcat cgaacatatc atcgtagaac ttcagccctt cttcgtttgg ctgagcttca
     6001 tcgccttttg gaaaaatgcg cgtccaggcg atggatgtac gaaaacattt gaagcccatt
     6061 tcggcaaata gcttgatgtc ttccttgtaa tgaccataaa aatcaacggc ttcatggttc
     6121 ggatagtatt ttcctggcaa cacttctttg gtgatttcgc gcggcacgcc gtgtgcgcca
     6181 ccggtcagaa cgtcacaaat gctcggccct ttgccgcctt tgttccagcc gccttcgacc
     6241 tgatgagcgg caactgcgcc gccccataag aaatctttcg gtaaggtgag ttttttcact
     6301 atcataggct ccagatgagt caatttttac aggaaatagt accatgcagc caaacgacat
     6361 cacttttttt caacgtttcc aggatgacat tctggctggg cgtaaaacca tcaccatccg
     6421 cgacgagtct gaatcgcact tcaaaacggg tgatgtgctt cgtgtcgggc gttttgaaga
     6481 cgacggttat ttttgcacga ttgaagtcac cgcaacctca accgtaacgc tggatacact
     6541 gacggaaaaa catgcagagc aggaaaatat gaccctgact gatctgaaaa aggtcattgc
     6601 cgatatctat cccggccaga cacagtttta tgtgattgaa tttaaatgtc tttaaattga
     6661 actcacgcct gttagttgaa atttcgcatt taatttcatg atttttcgga gattagtctc
     6721 aaatcagaat ttattttagc taacaggtgt tcactggaac tattctcagt tacgctggag
     6781 aggtatacat gtgcgtgtac gtttctccgg agtaagttat ggttcagaag cccctcatta
     6841 agcagggata ttcgctggca gaggaaattg ccaacagcgt cagtcacggc attgggttgg
     6901 tgtttggtat cgttgggctg gtgttgctac tggttcaggc ggtggatctc aatgccagcg
     6961 ccacagcgat aaccagttac agcctctatg gcggcagtat gatcctgctg ttcctcgctt
     7021 cgacgctcta tcacgccatt cctcatcaac gggcaaaaat gtggctgaag aaatttgacc
     7081 attgcgctat ttacctattg attgccggaa cctacacgcc gtttttgctg gtggggctgg
     7141 attctccgtt agcgcgcggg ttgatgattg ttatctggag cctggcattg ctggggattc
     7201 tgttcaaact gaccatcgcg caccgattca aaattttatc tctggtgacc tatctggcga
     7261 tgggctggct gtcgctggtg gtgatttatg aaatggcaat taagcttgcg gcgggcagcg
     7321 ttaccttact ggctgtaggc ggcgtggttt actcgctcgg ggtgattttc tacgtctgca
     7381 aacgcattcc ctacaaccat gccatctggc acggctttgt gctcggcggc agcgtgtgcc
     7441 actttctggc gatctattta tatattgggc aggcgtaatg ggtgtgcctt tgtaggcctg
     7501 aaaagacaca cccttgtcgc atcaggcgat aaatgcatga tgcgacacag agatttactc
     7561 ttcgagtgaa tacggcagcg gctcgatatg caatgtattc gcatcgtcac gcacgcggaa
     7621 gatgctgtcc ggttccatat cgttattcat gacgacctgt accacgacct gaccatcttc
     7681 cagttttacc gcagccagca cggttccggt acgacgccag ttctcgccca ttttcagctc
     7741 taagtcttca ccggcttccg gcagtcggct ggcgctaccc gtcagcaacc agagcgcgcg
     7801 tttgttggca ccacggaatt tagctcgcgc caccatctct tgtccggtat agcagccttt
     7861 tttaaagcta atgccgccca gcgcctggag gttggtcgcc tgtgggataa actgcccgct
     7921 gttggcggca tcaatcaccg ggaaacccgc ttcaatgttt aatgccagcc actgttggct
     7981 attgttcagt tccgcttcac cgcgcagttt atcggtcagc atattggcag tggcttcatc
     8041 ggttacgatc aggaaacgtt ctgccgggtg ttcaaaccat agcaaagtgg tcgcgccttc
     8101 tttaactacc tgtttttctt tcgaaggcag ttcactaaag atatttgcca gcgcggcgcg
     8161 cgcctgaaaa ccggcaacac caagcagcac acgctcgtcg tctggcgcga tggtcacttt
     8221 agagaacacc gcatattttt tcagttcagt cagctgcggt tcacgcacgc tgcgccgttc
     8281 aatccacgca aagccatcgc cgtcgcggaa cagacgtaaa ttgctccaca ttttgccttt
     8341 ggcgtcgcaa tgggcggcga gcaggtgctg atcttctgtc atctggctga catctgctgt
     8401 cacctgaccc tgcatatatt tttcgctgtc cgcgccagta atggtggcaa gcgcccagtc
     8461 atcaagcgtc atcagcgtca gcggtaaacg ggcagaagcc gtaggctgac ggggaggaaa
     8521 aggtgtaaaa gccataatag agtcctgagt tgcttaacgg cgtattaatg actaatggta
     8581 aaagagctat gaggcaatgc aagtgctttc actacgcgat ctgtgccttc tgatatgctt
     8641 tgcgaggcga cttccaaaaa aatgaagccc ttgaattgtt gcgaattaat ttcgcgatcg
     8701 cgattgtgtt tactgatatt cccgcaaaag aagtcgaaaa acacgttaca atagccgggt
     8761 actggatttt cgtgagacag gaagaagatg gacattaaca acaaagcccg cattcattgg
     8821 gcatgccgcc gtggtatgcg cgaactcgat atttcaatca tgccgttttt cgaacatgag
     8881 tacgacagct taagcgatga cgaaaaacgc atctttattc gtctgctgga atgcgacgac
     8941 ccggacctgt ttaactggct gatgaatcac ggtaaaccgg ccgatgcaga actggaaatg
     9001 atggtccgac tcatccagac acggaaccgg gaacgtggtc ctgtggcaat ctgatttgcg
     9061 cgtctcctgg cgcgcacagt ggctttcctt gctgattcat gggctggttg ccgctgttat
     9121 tttactcatg ccctggccgc tcagttacac cccgttatgg atggtgttgc tttcgctggt
     9181 ggtgtttgat tgcgttcgca gccagcgccg cattaatgct cgccaggggg aaattcgcct
     9241 gttgatggac gggcgtttgc gttggcaagg gcaggagtgg agcatcgtca aagcgccgtg
     9301 gatgattaag agcggcatga tgctgcgttt acgttctgac agcggtaaac ggcaacattt
     9361 atggctggca gccgacagca tggacgaagc tgaatggcgg gatttacggc ggattttgtt
     9421 gcaacaagag acgcaaagat aaagatgacg gcaaagcata tgcgctaagc cgtcagtgac
     9481 atcaggcgta atgctctgcc atttcgttga ggatttgctc gcaccagctc tgaatacgct
     9541 cgtcgctaag gtcatactgg ttagtttcat ccagcgccag acccacgaac agttgcccgt
     9601 cagcaatcac cggtttcggg ctggtaaatt catatccttc cgttggccag tagccgacga
     9661 acttcacgcc tttggtcgag agtttgtcat gcagcatacc gagcgcatcg aggaaccact
     9721 cgccgtatcc cagttgatcg ccaagcccat acagcgcaac aattttacct tcgaggttca
     9781 ggtcgtcgag ctgatcccag acggcttccc agtcttcctg gatttcgcca aaatcccagg
     9841 tcgggatacc cagaatgagc acatcgtact gctccattaa tttcggggag tcgtccttga
     9901 ggttatgtaa ggtcaccagt tctgggccga taatatcgcg gattttttct gccgccattt
     9961 cggtgtaaca ggtgctggaa ccgtaaaaaa gacccatatt cataacgtaa gcttctcgat
    10021 tctgctgtgg cttttgcggc tagtgtatca gaatcgcttt agtcacggca caataaggca
    10081 taatggatca aatgagtgac cataaggggc gcaagtgaaa caggatctgg cacgcatcga
    10141 gcagtttctt gatgctctgt ggctggaaaa aaatctggct gaaaatacgt tgaacgctta
    10201 ccgtcgcgat ctgtcaatga tggtggagtg gttgcatcac cgtgggttga cgctggcgac
    10261 ggcgcaaagt gacgatttgc aggcattact ggcagaacgg ctggagggcg ggtataaagc
    10321 caccagctca gcgcgtttgc tgagtgcagt gcgccgattg ttccagtatc tttatcgcga
    10381 aaagtttcgt gaagatgatc ccagtgcgca tctcgcttca ccgaaattgc cccagcgttt
    10441 gccaaaagat ttaagcgaag cgcaggtcga acgtttatta caggcaccat taattgatca
    10501 gccactggag ctacgcgata aagccatgct tgaagtgttg tatgctaccg gactgcgtgt
    10561 ctctgaactg gtcgggctga caatgagtga tatcagcctg cgtcagggcg tagtgcgggt
    10621 cattggtaaa ggcaacaaag agcgcctggt gccgttaggt gaagaggcgg tttactggct
    10681 ggaaacctat ctggaacatg ggcgtccgtg gctgttgaat ggtgtgtcaa ttgacgtgtt
    10741 gtttcccagc cagcgtgcgc agcagatgac gcgacagacc ttctggcacc gcattaaaca
    10801 ttatgccgtg ctggcgggta tcgacagcga aaagctgtca ccgcatgtgt tgcgtcacgc
    10861 ttttgccact cacttattaa atcatggtgc ggatttacgc gtggtgcaga tgctactggg
    10921 ccacagcgat ctctccacca cgcaaattta tacgcatgtc gctaccgagc gtctgcgaca
    10981 acttcatcaa cagcatcacc cgcgggcgtg atgtctgaaa agaacaggaa aatttatgaa
    11041 gaaaggtttt atgttattta ccttgttggc agcgttttca ggctttgttc aggctgatga
    11101 cgcggcaatt caacaaacgt tagccaaaat gggcatcaaa agcagcgata ttcagcccgc
    11161 gcccgtagct ggcatgaaga cagttctgac taacagcggt gtgttgtaca tcaccgatga
    11221 tggtaaacat atcattcagg ggccaatgta tgacgttagt ggcacggctc cggtcaatgt
    11281 caccaataag atgctgttaa agcagttgaa tgcgcttgaa aaagagatga tcgtttataa
    11341 agcgccgcag gaaaaacacg tcatcaccgt gtttactgat attacctgtg gttactgcca
    11401 caaactgcat gagcaaatgg cagactataa cgcgctgggg atcaccgtgc gttatcttgc
    11461 tttcccgcgc caggggctgg acagcgatgc agagaaagaa atgaaagcta tctggtgtgc
    11521 gaaagataaa aacaaagcgt ttgatgatgt gatggcaggt aaaagcgtcg caccagccag
    11581 ctgcgacgtg gatattgccg accattacgc acttggcgtc cagcttggcg ttagcggtac
    11641 tccggcagtt gtgctgagca atggcacact tgttccgggt taccagccgc cgaaagacat
    11701 gaaagaattc ctcgacgaac accaaaaaat gaccagcggt aaataattcg cgtgaaacaa
    11761 cagatacaac ttcgtcgccg agaagtcgat gaaacggccg acttgcccgc tgaattgcct
    11821 cccttgctgc gccgtttata tgccagccgg ggtgtgcgta gcgcgcagga actggaacgc
    11881 agtgttaaag gcatgctgcc ctggcagcaa ctgagcggcg tcgaaaaggc cgttgagatc
    11941 ctttacaacg cttttcgcga aggcacgcgg attattgtgg tcggtgattt cgacgccgac
    12001 ggtgccacca gcacggctct aagcgtgctg gcgatgcgct cgcttggttg cagcaatatc
    12061 gactacttgg ttccaaaccg tttcgaagac ggttacggct taagcccgga agtggtcgat
    12121 caggcccatg cccgtggcgc gcagttaatt gtcacggtgg ataacggtat ttcctcccat
    12181 tcgggcgttg aacacgctcg ctcgttgggc attccggtta ttgttaccga tcaccatttg
    12241 ccgggcgaaa cattacccgc ggcggaagcg atcattaacc ctaacttgcg cgactgtaat
    12301 ttcccgtcga aatcactggc aggcgtgggt gtggcgtttt atctgatgct ggcgctgcgc
    12361 acctttttgc gcgatcaggg ctggtttgat gagcgcggcg tcgcaattcc taacctggca
    12421 gaactgctgg atctggtcgc gctgggaaca gtggcggacg tcgtgccgct ggacgccaat
    12481 aaccgcatcc tcacctggca ggggatgagt cgcatccgtg ccggaaagtg ccgtccaggg
    12541 attaaagcgc tgctggaagt ggcaaaccgt gatgcactaa aactcgccgc cagcgattta
    12601 ggttttgcgc tggggccacg tctcaatgct gccgggcgac tggacgatat gtccgtcggt
    12661 gtggcgctgt tgctgtgcga caacatcggc gaagcgcgcg tgctggcaaa tgaactcgat
    12721 gcgctaaacc agacgcgaaa agagatcgaa caaggaatgc agattgaagc cctgaccctg
    12781 tgcgagaaac tggagcgcag ccgcgacacg ttacccggcg ggctggcaat gtaccacccc
    12841 gaatggcatc agggcgttgt cggtattctg gcttcgcgca tcaaagagcg ttttcaccgt
    12901 ccggttatcg cgtttgcgcc agcaggtgac ggtacgctga aaggttccgg tcgctccatt
    12961 caggggctgc atatgcgtga tgcgctggag cgattagaca cactctaccc cggcatgatg
    13021 ctcaagtttg gcggccatgc gatggcggcg ggtttgtcgc tggaagagga taaattcgaa
    13081 ctctttcaac aacggtttgg cgagctggtt accgagtggc tggatccttc actattgcaa
    13141 ggcgaagtgg tgtcagacgg tccgttaagc ccggccgaaa tgactatgga agtggcgcag
    13201 ctgctgcgcg atgctggccc gtgggggcag atgttcccgg agccgctgtt tgacggtcat
    13261 ttccgtctgc tgcaacagcg gctggtgggc gagcgtcatt tgaaagtcat ggtcgagccg
    13321 gtcggcggcg gtccgctgct ggatggtatt gcttttaatg tcgataccgc cctctggccg
    13381 gataacggcg tgcgcgaagt gcaactgact tacaagctcg atatcaacga gtttcgcggc
    13441 aaccgcagcc tgcaaattat catcgacaat atctggccaa tttagcgtca tcttctctat
    13501 aaaaaagagc gtggattggg tacaatcccg ctcttatcac cgcattttga ctagctcaat
    13561 aaaagaaatc agaccatgtt tgaaattaat ccggtaaata atcgcattca ggacctcacg
    13621 gaacgctccg acgttcttag ggggtatctt tgactacgac gccaagaaag agcgtctgga
    13681 agaagtaaac gccgagctgg aacagccgga tgtctggaac gaacccgaac gcgcacaggc
    13741 cctgggtaaa gagcgttctt ccctcgaagc cgttgtcgac accctcgacc aaatgaaaca
    13801 ggggctggaa gatgtttctg gtctgctgga actggctgta gaagctgacg acgaagaaac
    13861 ctttaacgaa gccgttgctg aactcgacgc tctggaagaa aaactggcgc agcttgagtt
    13921 ccgccgtatg ttctctggcg aatatgacag cgccgactgc tacctcgata ttcaggcggg
    13981 gtctggcggt acggaagcac aggactgggc gagcatgctt gagcgtatgt atctgcgctg
    14041 ggcagaatcg cgtggtttca aaactgaaat catcgaagag tcggaaggtg aagtggcggg
    14101 tattaaatcc gtgacgatca aaatctccgg cgattacgct tacggctggc tgcgtacaga
    14161 aactggcgtt caccgcctgg tgcgtaagag cccgtttgac tccggcggtc gtcgccacac
    14221 gtcgttcagc tccgcgtttg tttacccgga agttgatgac gatatcgata tcgaaatcaa
    14281 tccggcggat ctgcgcatcg acgtttatcg cgcgtccggc gcgggcggtc agcacgttaa
    14341 ccgtaccgaa tctgcggtgc gtattactca catcccaacc gggatcgtga cccagtgcca
    14401 gaacgaccgt tcccagcaca agaacaaaga ccaggccatg aagcagatga aagcgaagct
    14461 ttatgaactg gagatgcaga agaaaaatgc cgagaagcag gcgatggaag acaataaatc
    14521 cgacatcggc tggggcagcc agattcgttc ttatgtcctt gatgactccc gcattaaaga
    14581 tctgcgcacc ggggtagaaa cccgcaacac gcaggccgtg ctggacggca gcctggatca
    14641 atttatcgaa gcaagtttga aagcagggtt atgaggaacc aacatgtctg aacaacacgc
    14701 acagggcgct gacgcggtag tcgatcttaa caatgaactg aaaacgcgtc gtgagaagct
    14761 ggcgaacctg cgcgagcagg ggattgcctt cccgaacgat ttccgtcgcg atcatacctc
    14821 tgaccaattg cacgcagaat tcgacggtaa agagaacgaa gaactggaag cgctgaacat
    14881 cgaagttgcc gttgctggcc gcatgatgac ccgtcgtatt atgggtaaag cgtctttcgt
    14941 taccctgcag gatgtgggtg gccgcattca gctgtacgtt gcccgtgacg atctgccgga
    15001 aggcgtttac aacgagcagt tcaaaaaatg ggacctcggc gatatcctcg gcgcgaaagg
    15061 taaactgttc aaaaccaaaa ccggcgaact gtctatccac tgtaccgagc tgcgtctgct
    15121 gaccaaagca ctgcgtccgc tgccggataa attccacggc ttgcaggatc aggaagcgcg
    15181 ctatcgtcag cgttatctgg atctcatctc taacgatgaa tcccgcaaca cctttaaagt
    15241 gcgctcgcag atcctctctg gtattcgcca gttcatggtg aaccgcggct ttatggaagt
    15301 tgaaacgccg atgatgcagg tgatccctgg cggtgccgct gcgcgtccgt tcatcaccca
    15361 ccataatgcg ctggatctcg acatgtacct gcgtatcgcg ccggaactgt acctcaagcg
    15421 tctggtggtc ggtggcttcg agcgtgtatt cgaaatcaac cgtaacttcc gtaacgaagg
    15481 tatttccgta cgtcataacc cagagttcac catgatggaa ctctatatgg cttacgcaga
    15541 ttacaaagat ctgatcgagc tgaccgaatc gctgttccgt actctggcac aggatattct
    15601 cggtaagacg gaagtgacct acggcgacgt aacgctggac ttcggtaaac cgttcgaaaa
    15661 actgaccatg cgtgaagcga tcaagaaata tcgcccggaa accgacatgg cggatctgga
    15721 caacttcgac tctgcgaaag cgattgctga atctatcggc atccacgttg agaagagctg
    15781 gggtctgggc cgtatcgtta ccgagatctt cgaagaagtg gcagaagcac atctgatcca
    15841 gccgaccttc attactgaat atccggcaga agtttctcct ctggcgcgtc gtaacgacgt
    15901 taacccggaa atcacagacc gctttgagtt cttcattggt gggcgtgaaa tcggtaacgg
    15961 ctttagcgag ctgaatgacg cggaagatca ggcgcagcgc ttcctggatc aggttgccgc
    16021 gaaagatgca ggtgacgacg aagcgatgtt ctacgacgaa gattacgtca ccgcactgga
    16081 acacggctta ccgccgacag caggtctggg aattggtatc gaccgtatgg taatgctgtt
    16141 caccaacagt cataccatcc gcgacgttat tctgttcccg gcgatgcgtc cggtgaaata
    16201 agcattacgt tatgctcaca accccggcaa atgtcggggt tttttaattg tgctgcgcga
    16261 aagcagacaa caattttctt gcttcactat tggctgcctg catcaccatc cacggactga
    16321 acgcccacgg cgtggcatca ataccgtgta atacatctgc taaatcacac cattgataat
    16381 ccatcacttc atcatcgttg atctgtaacg cactggtggt gcgtgcggca aataccggac
    16441 acacttcatt ttccacaatg ccattcggat cggtggcgcg atagtagaag tcaggataga
    16501 tagattcagg aggcgtaatt tccacgccaa gctcataacg gcaacggcgg atcaccgcgt
    16561 cttcgttgct ttctcccagt tgtgggtgcc cacaaaccga gttagtccac acgccaggcc
    16621 acgctttttt gctaagggcg cggcgggtaa ctaataattg ccctttggca ttaaacagcc
    16681 aactggagaa cgcgagatgt aagagagtgt ctgccgtgtg tgcggcatac ttttccagcg
    16741 tacccgtggg aactccctgt gcattcaata aaatgacgtg ttccgtttgc ataatttctc
    16801 acatgtaatt ctaatcactc atcattatag ataattgaag agtgaaatga ttaaccaagt
    16861 gagcgtttat cgacaaccgc ccgttttgag cggatgtcga caggtaaaaa ccatttaaaa
    16921 aagtagcatc atatatttga agcgactttc aatggccatt gcaacacagt cagaaatgac
    16981 aggaatagag agagtaaaaa gcaacagcag actgaatata gcgcgcatta aaagattcat
    17041 attattctcc atgctcattt ttcttcagct tgcgcatcag aagccacata cgtactgtcc
    17101 tgacttttaa atctttatca gagacggatt ccttcaccag atcagcatga tgctgatagc
    17161 cattaaagaa gacgttcagc acaacggcgc taagggttgc gagcataatg ccgctatgca
    17221 gcaacggttg cagtacggcc ggtaacttag aaaagaaatc gtgagagagc gtcggagtca
    17281 tgccaacgcc gagactgatc gccacaatat agaggttgta acggttggtg gtgtagttac
    17341 agcgcgacag aattcgaatc cctgtcgcca gtaccatgcc gaacatcacc agaccagcgc
    17401 cgcccagcac aaattgcgga atggaggcga ccagcaccgc catttttggc accatgccaa
    17461 acaggattaa tataattccc gaagaaatac acacccagcg gctatgaacg cgcgtcacgc
    17521 taaccaggcc aacgttttga gaaaatgacg tatgggggaa gctgttaaac gttccgccta
    17581 tcattgtccc tacgccatcg acacgcagcc cgcgaataat atcgtgtgaa gagagtttac
    17641 gaccaactat ttcacccagt gccaggaaca tccccatcga ctcgataaac acgatgatta
    17701 acacggcagt catggtcaga atggaaacgg gatcaaaaat cggcatacca aacgacatcg
    17761 gggtgacaat cgcaaaccac gaggcatcat gtagcccgga taaattgact tcattcatca
    17821 tccacgaaag taaaaagcca aatacaatcc ccagtaatac ggcgacgttg gacataaatc
    17881 ctttcgcata gcgagtaatg agcaagataa aaattaagac ggcaaaggag atacctaaat
    17941 aaacgggatt accatattgc ggattccctt tacctcctgc ggcccagtca atacccacct
    18001 gaatgatgct aagtccgata gaagtaataa ccacaccggt aaccagtggc gggaataaag
    18061 gcatcaagcg accgataagt ggcgctaata atgtggtgat aaaacccgcg gcgatagtgg
    18121 caccaaatat ccccagcagg ccgatatccg ggttcatccc aatggctatc attggtgtta
    18181 cagcggcgaa ggtcaccgac ataatcaccg gcaggcggat ccccataaag cggccgatac
    18241 cgatacattg caataatgtg acgatcccgc agcaaaagag atccgagcta atgagcatcg
    18301 caatagcttc ttttgagagg cccagtcggt cgccaatcat aagaggaaca gcgactgcac
    18361 ctgcgtacat gacaaggacg tgttgcagac cgagtatgat cagctttcct ggtgataata
    18421 tgcggtcaac ctcatcagtt gggcggtctt gcccagaaga tgagggaagt tgggaatcta
    18481 tggcgctcat cagtgtttct cctttcgccc gccagagtaa aaagcaaccg tttacgttca
    18541 tgaaactgtc taaatacatg ctcgtggagt gccagttttg cgaggaaggg gagttagaga
    18601 caggaattgc ggaggtaatc acataatcgt ggttaaacca cgaaaatgag aagagaacct
    18661 gacaatggaa ttatctgtaa ttttctcata ttgatatttc attaatcatt ctggcatgtc
    18721 gcactctcgc atttaatcgt ttttatctgg atagcattct tttgatcggc aatgttgaaa
    18781 taatgaaagc tgcatagatt gaggggcaat aatttgaact cagtcgcaaa attataatta
    18841 aatcggccat gctacctaat acatgcccat ttattgcatc gcgaaatttg agaagacata
    18901 actcattaaa tgaaagggat gcaaatgaat aagtttatcg ctgctgaagc tgcggaatgt
    18961 ataggctgtc atgcttgtga aattgcctgt gcgatggcac ataatcaaga aaactggccg
    19021 ctgagtcaca gtgactttcg accgcgtatc cacgttgtag ggaaaggcca ggctgcgaat
    19081 ccggtggcct gccatcactg caacaatgcc ccttgcgtta cggcttgtcc ggttaatgct
    19141 ctgactttcc agcccgatag cgtacaactg gacgagcaaa aatgtattgg ttgtaaaaga
    19201 tgcgcaatcg cttgcccctt tggcgtcgtt gagatggtcg atacgattgc gcagaaatgc
    19261 gacctttgta accagcgcag ttccggcacg caagcctgta ttgaagtctg cccaacacag
    19321 gcgttacggc tgatggacga taaagggttg cagcagataa aggtggcccg ccagcgcaaa
    19381 acggcagcag gaaaagcgtc atcagacgct cagccatctc gcagtgcagc gttgctcccc
    19441 gttaactcgc gtaaaggcgc agataaaatt tcagcgagtg aacggaaaac ccactttggc
    19501 gaaatctatt acgggctgga tccacaacaa gcgacttatg agagtgaccg ctgtgtttat
    19561 tgcgccgaaa aagctaactg caactggcat tgcccgctgc ataacgctat tccggattac
    19621 atccgtctgg tgcaggaagg aaagattatt gaagcggcag aactttgcca ccagaccagt
    19681 tccttgccag aaatctgcgg cagggtatgt ccacaggacc gtctttgtga aggcgcatgt
    19741 actttgaaag atcactctgg cgcagtctct atcgggaatc tggaacgcta cattaccgat
    19801 accgcgctgg cgatgggctg gcgtcccgat gtcagcaaag ttgttccccg tatcgaaaaa
    19861 gtggcggtga ttggcgcagg gcctgcaggg ttagggtgtg ctgatatttt ggcgcgcgca
    19921 ggagttcagg ttgatgtctt tgatcgccat ccagaaattg gcggtatgct gacttttggc
    19981 atacctcctt tcaaactcga taaaacggta ttaagccagc ggcgagagat attcaccgca
    20041 atgggaatcg actttcatct taactgtgaa attggccgcg atattacctt tagcgattta
    20101 acttctgaat atgatgcagt ttttatcggc gtggggactt acgggatgat gcgagcagat
    20161 ctgccgcatg aagatgcgcc cggtgtcatt caggcgttac cgttcctgac tgcccatacc
    20221 cgccagctca tggggttgcc ggagtctgaa gagtatccgc tgacggatgt ggaaggtaag
    20281 cgagtcgtgg tattgggcgg tggcgatacg accatggatt gtttgcggac ttccatccgc
    20341 ctcaatgccg cctgcgtgac ctgcgcgtat cgtcgtgatg aagtcagtat gccgggctcg
    20401 cgcaaagagg tggtcaatgc gcgcgaggaa ggtgtcgagt ttcagttcaa tgttcagccg
    20461 caatatatcg cttgtgacga agatgggcgt ttaactgcgg tgggcctgat tcgtaccgcc
    20521 atgggtgagc cggggccgga tggtcgccgt cgtcctcgtc cggtagcggg ttcagagttt
    20581 gaattgcccg ccgatgttct cattatggcc tttggtttcc aggcgcatgc catgccgtgg
    20641 ttgcagggca gcggaattaa actcgataaa tggggcctga ttcaaaccgg tgacgtcggg
    20701 tatttaccta cccagacgca tctgaaaaaa gtctttgctg gtggtgatgc tgttcatggc
    20761 gcggatctgg ttgtcactgc aatggccgca ggaaggcagg cggcgcgcga tatgttaact
    20821 ctgtttgata cgaaggcatc atgatgaaat cgttaattat cgttaatccg gctgactgta
    20881 ttggctgccg tacttgtgaa gtagcctgtg tggtcgctca cccttcagaa caggagttga
    20941 atgccgatgt ctttttaccc cggctgaagg tacagcgact ggacagcatc agtgcgccgg
    21001 tgatgtgcca tcagtgtgaa aacgcccctt gtgttagcgc ttgccccgtg ggggcgctga
    21061 cgatgggcga gcaggtggtg cagaccaatt ctgcccgttg tattggctgt cagagttgcg
    21121 tcagtgcgtg cccgtttgga atgatcacca ttcagtcgtt gccgggggat acccggcaac
    21181 aaatcgtgaa atgcgatctc tgtgaacagc gggaagaggg gccagcctgc gttgaatctt
    21241 gcccaacaca ggcgctgcag ttgcttactg aaagagaact caggcgagtc cgccagcagc
    21301 gtattgttgc cagcggtgag aatcctctgt gaaagaaatc tccgcgctga aaggcgcgga
    21361 ggatctatta gattgcccaa ccacccgcgt aaaatgcgac cagtgcggca gtaatgatca
    21421 ccgtaccaat attcagcttt tgccattcgc gtgcaaagac gcggcctacg accagtgtca
    21481 caaagccaag cataataccg gtaacgatat tacaagtcag aacgatgaac acggcacaca
    21541 ccaggccagc cattgcgtca ataaaatcat tgaaatccag cttcgagacg ttacttaaca
    21601 tcagcaaacc tacgtacatc agagcggggg cagtggcgta accagggatc aaaaatgaca
    21661 gcggtgataa gaacagaatc agcaggaata acgcccccac tacggttgcg gttaaaccgg
    21721 ttttcccccc ggcggcggtt cctgccgctg attcgatata aaccgccgcg ggcgctgcgc
    21781 ccaccaggcc ggagaatatt gaacttactg agtcactggt cagggctttg ccgccgttga
    21841 tgatctggtt gtctttatcc aacaaattcg cctgaccggc gacggcacgg atggtgccag
    21901 tggcgtcgaa cactgcggtc atcaccaatg ccagtacact cggaagtaca gttggctgga
    21961 gtgcgcccat aatatcgagg ctgaaaatca gagacttacc atcttcgcca gtcaggcttg
    22021 gcatcgccac cagaccgtgg tacttcaccg ctgggtcaaa gattaagccg atgatcgaaa
    22081 ttgcaataat caccaacaag atcccgccgg gtacgcgaca cttctccaga ccgaagataa
    22141 ccgccagccc cagcaagctc atcatcaccg ggaaggaggt aaacgcaccg agcgccactg
    22201 gcaagccttc aatcgggttt ttgataacca ttcccacacc gttagcagca atcagcagca
    22261 gaaacaggcc gataccgata cctgtaccgt gagcaatccc catcggcaaa ttacgtaaga
    22321 tccaggtacg cacaccggtt acggaaatgg cggtgaagat gacgcccatc agaaataccg
    22381 cgcccagtgc gacaggaacg ctaatttgtt gcccgagtac cagactgaat gcggtaaacg
    22441 ccgtcaagga aatcgcacaa ccaatcgcca ttggcaaatt ggcccacaac cccatcagca
    22501 acgagccgaa gcccgcgacc agacaggtgg caacaaacac agctgcggga ggaaaacctg
    22561 ctttgcccag cattcccgga acgacgataa cggaataaac catggccaga aaggtcgtta
    22621 agccagccag tacttcctga cgaacggtac tgccacgagc ggtaatttta aaataattat
    22681 ccagcgcgcc ctgtggcttt ggtgcgtccg gtgtttgtag gatgtctcca gacataatga
    22741 tgtcctcacg aggatgttaa agttttatta ttagttgcgt tcgtacacca gacgaccatc
    22801 aacgtaggtg cggtagatcg aacggtcatc gcccaacgtc atcatcacga acaatttgtc
    22861 gactaaagaa acagagttgt catagcgcag ctgctgtagc ggagtggcag tgggttccat
    22921 caccacgaaa tcagcctctt tgccaggtaa aaagttgcca atcaaatcgt caaggcccag
    22981 agatttcgct ccgccgagcg tggccaggta aaacgcttca tatgccgaga ggcgatagcc
    23041 ttgtaattgc aataccttgt aggcttcgtt cagcgtttgc agcatgttaa aagttgttcc
    23101 ggcaccgata tccgttccca tgcccacttt gactttcttc tgccatgctt ttttcaagtt
    23161 gaataagccg ctgccgaggt aaaggttgga ggtcggacag aaagcaatgc tggatttggt
    23221 ttcgctgaga cgatcccact ctttttcttc gagatggacg cagtgagcaa agacacagtt
    23281 tttaccggtc agaccgtact gatggtaaac atccagataa ccatcatggt caggataaag
    23341 cgatttgacc caggcaattt catctttgtt ttcacagaga tgggtatgta cccacgtatc
    23401 cggatattct tctttcaggc gttgcgccat cgccatctgt tcaggagatg aggtaggggc
    23461 gaagcgtggc gtaatcgcat acaacagacg accatttttg tgccagcgtt caatcagttc
    23521 tttgctttgg tgatagctgc tttcggcagt gtcgagcaga taatccggtg cgtttcggtc
    23581 catcatcacc ttaccggcaa tcatacgcat attgatatga ctggcggctt caaacagcgc
    23641 atcaacggat tgtggatgaa cagtgccaaa caccagcgcc gtggtggttc cgttacgtaa
    23701 aagctgcttg atgaagaacg ccgacatctc gcgggcgtac tctaaatcct cataacgacg
    23761 ttcagtaggg aaggtgtgtt tattcaacca ctccagcaat tgttcaccat aggcccccac
    23821 catttcactt tgcggataat ggatatgtgt atcgacaaag cccggtacta tcagtttgcc
    23881 gcgatagtcg cgcacgcgaa tggtgtcagg aatttgatgc tttccgtctt cccattcgcc
    23941 aaaccattcc acttttccct gtttaatgag taataaacca tcctcaataa accgcagcgc
    24001 agaggcaatt tcttccgggt tatcgaccgt acgggtgaca tcaataaaac tgcctcgtac
    24061 cgcttttaac gtgtgttctc ctgacatcat tgactccttt actgttaatc catctcttcc
    24121 gctgaggtaa taccaggcag aacgttttcc tgtcggtagc caccagggag gataatattt
    24181 aataaaatcg cagttaaccc gccagcacaa atagggtttt caactaatac ataaatagag
    24241 gctggcaata ttttaaaaat ttcgggatcg taggagacgc caagccctaa acctaaagac
    24301 gtggcgacaa taagtgtttc tcggcgcttt aatccgttgg tgatgatgat gcgaatccca
    24361 gcgatggcaa tcatggaaaa catcaacgtc attgcgcctc ccagaactgc cgagggaatg
    24421 gtcgtgaaga agccgccaat catcggaaat aagccgagga taaccagcat taccgcgatg
    24481 gttcgcccga cataacgtga agcgacgcca gtcatctgaa taaccccatt attttgcgca
    24541 aacgtggtta atggtaatga accgacagcg gaggcgataa cagaaaccag accatctgcc
    24601 agcacgccgc ctttcagtcg ggactgatac tcttccccct gaatggggcg gcgggaaacc
    24661 attgccgtgg cggtgatatc gccgactgct tccagcacgc taagcagata aatcgtgcca
    24721 accaccagga actgatggaa gctaaaacta aagccgtatt tgaacggatg cgggatggtg
    24781 attaacggca aattgcgcat actgctgaaa tccaccatgc ccaggcataa cgatgcgata
    24841 tagccgacac atagcccaat ggcgatccct cccatgcgta gcaacggact gcgacagcag
    24901 ttaaagccga tcaccacaat taaaaccaat aaaccaacgc cgagatgttc gtaattgccg
    24961 aacgtaccgc tgcttttggc tgcaaatcct ccaccaaaat cgataatgcc gactttaatc
    25021 aggcttaagc cgatcatcag taccacaata ccgctgacgg taggcgtaat aacccgacgt
    25081 aaatagggca ggataaaaga agagccgaca accagaaatg cgccaacgaa ggagacgccg
    25141 aggagcgacg acatgattaa ctcttcgtga aaaccgtcgc ttttcatgct gctgcccagc
    25201 gcaatcatga ccgtaacaaa tgaaaaattg actgactgaa ttgagagaag accagaaccg
    25261 acgatgccgt agcggtttac ttgtaaccag gtaccaatac cagaggcgat catcgccata
    25321 gaaacaagat aggcagttgt ttcagcggaa agctgtaagg tcgcacccac gattaacgcg
    25381 ggggttacca tcggaacgaa aattgccaac agatgggtaa tggcacctac gagagcctga
    25441 tgaaagggag ggcgatcctc cagttcaaat ataaggtcag aacctgcatg gtttatatca
    25501 gacatccctt cactcctttt aaagtaaatt tgatcatctg aattagtaaa ataaattccc
    25561 acgaatttgg ggcgcaggac gccttgagaa cggcttatct cattttcgca gtcactattt
    25621 agtcatcgtt caaacagaga aacaggccgc cgaagcggcc atttattact tatatttttt
    25681 ccagcgcggt gagtattttc tccggtgtga aatgccattc gcgtaaccag atgccgcagg
    25741 catcgtgaat tgcggtagca atcgccggag cggcgccatt tacaccgatt tccgagatcg
    25801 atttcgcccc gaatgggccg actttatcgt cgctcggcac cagcacagcg cggaaatcac
    25861 gcggaatgtc accaattttc ggtgcgccgt aactgcgtaa atcacgcgtt aacgggtgac
    25921 cttcggcgtc ataaatgatc tcttcgctca tactgtggcc gatagctcgc agagtggcac
    25981 cgtagatttg tcccaacgct aactctggat tgaccggtgt accgcagtcc agcaaggcgt
    26041 agaatttatc caggcggatt tcacccgtac gcgtgttgac ggcaacttca gcgaagtttg
    26101 cgccatacgg gaaggcgaaa tcaggcgtga tataactgcc agttcccacc agtgaaccaa
    26161 agccggtgcc ggtttcgcct ttatgggcta tctctccgaa actaacttcg cctttcttgc
    26221 cgcgtacgac gcccggcgtt gctagttgga catctgccac tggctcaccc aacatttgcg
    26281 caccgtggaa cagaattttc tcccgcagat tttctgctgc caaacgcgcc gcgttacccg
    26341 agaagcaagt accggacgag gcatatgcgc ctttatcaaa caacgcatga tcggtatcac
    26401 cggagataac atgcacgtcc tgcggtgggc agtgcagcac ttctgctgcc agtttcgtca
    26461 ccacagtatc cagaccagta ccaatatccg caccgccaga atgaacgata aaggtaccgt
    26521 ctgattccag tttgatcatg cagttagcct gatcgatatc tgggatccct gatttctgca
    26581 tgataatggc gacgccacga ccgatgtgcc agtcaccgtt ttgtggtttt ggtgaagacc
    26641 attggatcat ctcgcgaccc tgacgcagga tctcttccag tgcgcagctg gcggcggaag
    26701 gaacggaggt cggcgcttta ccttcaccga ttgcaccgag aattttcagc tcttgccctt
    26761 cgtgtacccg gttacgttcg ataatttcca gttggtcgat ctgtaactgt tcagccagtt
    26821 ccgctaatgc catggtgata gcgaagttac ctttcggtgc gccataaccc tgataagcac
    26881 catttgggca aatgttgctg tagtaggtgg tgacctggaa atcgacgtta tcgcacggat
    26941 ataacggcag cgacagcgcc ggtccgttac atggtacggt gagtgagtgg ttgccgtaag
    27001 ggccagtgtt ggcgcggaaa tccatcttca ctgctgtcag gcgaccatct cttttcgctc
    27061 ccagtttgac ggtgactttc gcaacgtgac gagaggtgtt agcaataaac tcttcttcac
    27121 gggtgtagcg gaacagtacc ggacgcccgg tcacgcaggt tgcccaggcg cacacttctt
    27181 ccagcaggat gtcctgttta gacccaaaac cgccgccaac tcgctcttta atgacatgaa
    27241 ctttatgttg tttcatgccc acgaggcgcg cgacctggcg gcgtaagtgc catggtacct
    27301 gggtggaggc gtggatcacc agacgatcgc cgtccatacg ggtaaagcag atatgtgttt
    27361 cagtcgggca ctgctgtgcc tgcgttgagt tataggttcg ctcaatgatc acatcggcat
    27421 cggcaaagcc tttgtccata tcaccaatat gaccatgaat actggcagcg atatttttgc
    27481 gtgggcgaga accgatcggg aagttgatga tcatatgctc gccgcgctgg gctgcatgac
    27541 agttatcttc ctccagagta tctggtgcac cagcaacata caccaccggt tcatcgtgca
    27601 cgacaggcgc atcttccgcc attgcctcgt caatcgacat taccggctta agcacttcat
    27661 attcaacgtc gatgagcttc agtgcttcga gcgcaatgtc ttcactttca gcgacgaccg
    27721 cagcaacgcg atcgccgacg tgacgcattt tcttgccgaa cattcggcgg tcaagcggtg
    27781 acggttccgg tgcgctctga ccacccgggg tatagtaaat atccgggcaa ttcaggtgag
    27841 taataacgtg aacgacgccc ggtaaggctt cagctttgct gacatccaga tgagtaatca
    27901 gtgcgtgagc gtgtgggcta cgtaacattt taatgacgca ggcgtcagcc gttacgcgat
    27961 cttcaacata gcagggtttc gcctgcacca ttttcgcggc atcagtttta ggataatgct
    28021 tgccaatgac ttcaaggtcg tcacggaaag tcggagcgat atcaatggtg gcctgcggat
    28081 tatttttacg tgcaaccgcc agttcaatga cctgatagta ttgctgccag ccagcatcgc
    28141 ggctaaacag acctgagagc gcgtcgtcga tctcttcgcg ggtaggtgcg tcgatgcgat
    28201 cgagaagatc ggtgataatc agagccgcag ctggatcgtt ataaccagac tgcaccacgc
    28261 caacatcaac catggcctgt tgaaccagac ttaactcatt ccatttgccc agagattctg
    28321 cggtacgaat atctgccttc tctaactgtg cggcgataag caaggacgcg ttaacgatat
    28381 taccgttaaa gataattgcg tcagaaccgg caaacccaaa gccatcatcg ctgttgcgta
    28441 cagagtgcat tcccatgtta aacaacagct tttgcacgtt ttcaccagga ttaacggtta
    28501 gctcctgggg cgtgccattt aaagtaaagt ggatgatcat acggcttcct ccccagcctg
    28561 ttggcagtcg gcataaaggt cggctaccag aactcccgtg atatagcgtt tataggccac
    28621 gctgccccgc aaatcttcct gcgggaaaat ggcgttggcg acagcctgtt caagggcatc
    28681 accttccagg ttttgttttt caacatcgtg cagacgcagt gctttactgg ctacgccatc
    28741 cagcgcaatt cgcataccgt cgtggtctgt tattgcaacg gctgccgtca cgacggttaa
    28801 acctgcctga gaacggctaa ttttgcgagt cgcacaggtg cgatacggat ctttaatgat
    28861 gatttcggtt agcaggcgat cgcatgggca ggccaggtac tcctcaattg acagagtttc
    28921 gccgttgcca aaaaccagtt cagcatccag tgccagcagg acgggaagca gcactgactc
    28981 ttcttgccgg gcggcaattt caccaccaat ggtcgactga ttacgaacat ggcgtgagta
    29041 aacaaaaccg agagcttcac gcagtgctgc aggaataaat cgcgcatcac gcagtgtttg
    29101 caagcgagac atcgcgccaa tccgcagtgc accgttatcc cagtcaaccc agtccagttc
    29161 cagatcctgt aaggaaatgg caatcttttt atcggtacgg gttggtgtag cgttgagttt
    29221 gctgcccccg gcgaaccaga cggcttcatc ctggtagcgg cgcttcagtt ccagcgcctg
    29281 ttcgacagag tcgggtctga aaaattgttc aatcatcgtc tttccttaat ttcccctctt
    29341 ctctgaaaga agaggggagc tgttatttat gccagcgcat ccatccgacg ccacatactg
    29401 gcagcggctt ttctggcttg cgcataaatg gaatcgcaat cgaagttaaa ctgacggtct
    29461 tcatagacca tcacaccatt gaccatcacg ctgtgaacgc tgcctgagcc cataccgaaa
    29521 gcgatatgcc cggcgatatt gtctgccagc agcggcgtcg gcgagttgta atcgcaaatg
    29581 gttaaatcgg ctttgtaacc ggcttccaga aggccaaatt tcgcgccaaa gttgcggctc
    29641 atcagttcgt tgccgttagc cagagctttg gcaaagctgt caggccacag cggaccaccc
    29701 gcatcgcgat gtttaaagaa ggcaaatttc atctcttcaa acatatctga accaataccg
    29761 tccgtaccca acgccagatt gcggatgtcg caaagatgat ggttgtagcc gacatggttg
    29821 ttcatgtttg aacgggcgtt atgcaccagg aatgcatcac gctgattgag caacgcgatg
    29881 tcgtctttag acagataaag cccatgagcg accagcgttt tgctgtcgat gagatcgaat
    29941 tgcgctaatc gtgccagcag atctttgccg taccagtgat gactatggga aacatcgtaa
    30001 aggtcttccg cagcgtgaat atgtaaacca cggcctgtgg ctttcacggc ctcacgcagc
    30061 atttccagac cggcatccgg cacagtaaac ggcgcgtgag caccaatatg agcctctacc
    30121 agatacggtt cagttgccgc tttcttcgca tcatcaatct gacgggcgaa gcggatgttt
    30181 tcttctacac cttcctgcaa ctctttgata ccgttgttac ggtcagtagt ttcaaaacag
    30241 gtcatcgcgc gcaggccaac ttttaaaaat gcgttgcgca atgtggagag cgacccgtcg
    30301 atatacgccg gagaggcatg gtgatcgata accgatgtac atccgccctt aatcgcttcc
    30361 agggaacaaa tcagtccgct gtaatagagc gactcttcat caagggcgcg atcgagtcgc
    30421 caccagagat ttttcagcgt tgagatgaaa tccgggcagg gggcgatgtt tgccataatt
    30481 ccgcgggaca gccccgagta aaaatggttg tgcgagcaga caattcctgg catcacaatc
    30541 cgaccatgca tctctttgta gctggcatcg gggtagcgtt gcgtcagggc atcgccgata
    30601 gcgacaatca catcattttc gatggcgata tcaacgcctt cctgcacttt cgctgggtgt
    30661 aactgcactg cggtgacatt cttcagaatc aacatgatta cacctccaca cggcccagca
    30721 gataatgatg atgctggtgg acatggctaa tgatgcggca catatcgttc agctccggtg
    30781 gtacgttgtt gaactgacct tcgttgtcga tgtttaacac ccagctttgg ttattcagac
    30841 gtacgcgtac ccggcaatct tccacaagga agcctgggtt gctgctgtta tcaaagtctt
    30901 gcgacaggct gaagacggtg attttgtctt tgtacggttt accgttccac gggcagaatt
    30961 gggcgcagtt gccacattcg ttacagtaag cgtcgaggtg cagcgtctgg aaacggttct
    31021 ggaagcctgg gaccgcaatg gatacgttgg cgcggttcgg gcagacatcc acacacttgc
    31081 tgcaaacgta gttacattca aggcagcgtg cggcttcctg ggcgacaaac gcgtcacgat
    31141 cgtcgctatc caccagggtg acagagatat cgcctttgcg ttgatagatt tccgccggat
    31201 taacgttgtt ccagtattta tcgttctggt gggaacggat attttcccga ctaaggatgg
    31261 catcggtcgc ccgacgcgcg gttccgacag cagcgacaat ggaggatggt ccgcgctgta
    31321 cgtcgccgat cataaagacg tcagtcagac gagtttcgcc attatggtcg acgtctggcc
    31381 agccgttttt gtccagcggc acgcccatcg cattcagggc ttcagtatcc tgctgttcac
    31441 caatggcggt gatcaggctg tctacatgca gtgttactgt ttcgttggtt tcaaccggac
    31501 ggcgacgacc tttctcatca ggttcgccaa gcgacataac gcgcaaggtt aaggtgccat
    31561 cagcatcgaa acgttccgga ttattcagaa aacggaactc cacgccgtcg tgcaacgctt
    31621 cttcatactc ttcgcgccat gctggcatct cttgcagtga acgacggtaa acgaccgttg
    31681 ctttttctac gcctggaacg cgtaacgccg cacgagcgca gtccattgcg gtgttacccg
    31741 ccccgacaac gaccacatgt ttgcccagct tgagctctgt gcccttgttg tattcacgca
    31801 ggaaggggag tgatttccag acattttggt tgtcgcccgc cagtttcaca ccgctatttt
    31861 tatcagtgcc ggtggcaatc agaacatagt ggaagccctg attttttaac tgttcaacgg
    31921 tcagatcggg tgagcagcca tactcaaatt tcacgccgtg agcggcaaca aaatcgatat
    31981 cgtgctgaat taactctgga ggaatacgga actgaggaat gatatttttc accacgccgc
    32041 ccgcgttggc ttcgcgttca aacagcgtaa ccggatggcc cgctctggca aggaagtaac
    32101 ctgcggccag acccgccgga cctgcaccaa tcacggcaac cggatggcgt gaaccagaac
    32161 cggctggttt gtgccagcgt tgcttatatt catcccaacc tttttccagc gcgacttttt
    32221 tcagttcgcg gatattcagc gcactgtcgt aatccaggcg ggtacagttg tattggcact
    32281 ggtgatcgca aatatgaccg gtaatggcgg gcagggcgtt gcgttgatag atgagttcca
    32341 gcgcgtcggc atagcggtgt tcgccaagca gacggatgta ttccggaata tcttgcttga
    32401 tagcgcaggc agtaacacag ggggcaacgt agcaatcggt cagcggcagg tcttcagcca
    32461 cttcaatacg ctcttctggc ttccagtgtt tctgggtgta ttccatggtt aacgcgtctg
    32521 ctgccagtct gttcagtcgt tcgacgtcaa cgtggtcaag tccccaggcg tcggagcctt
    32581 ccagctcgcg catgcaggca cttaagcgca gatagccgcc aggtttcagc aggtcggttg
    32641 ccatagtaat agggcgaata ccagtatcaa aaatatcgcg gatagtcagc tgactggcac
    32701 caccggaata agaaatgggc agtttgccgt caaaggcgcg agagagaact gctgcaacgt
    32761 tgatggagag cgggaacagc gcacggcctg acatatacat ctcttcaccg ggcagtgcgc
    32821 ctttgttatt aatggtgccg agagtgttag tcagttttac gccaaagccg agtgattttt
    32881 cttttgccag tgccatcagg cgttccagca tttccagtgc ttgcgtcagc ttgaggtcgt
    32941 gatcaaatga ctcttctttt aagccgatgt aaccgaaacc gcagacatcg aggatctcac
    33001 gaacacgcgc gtaccccagt aaggtcgggt tgagtttcac aaaggtgttg agcccttttt
    33061 cttccagcat gtagcggcaa atggcttcga tttcatgcgg aggacagccg tgcatggtgg
    33121 agagggtaac cccttgtacc atgctggtgg ggatgcgagc gggtaaggct tgcaagcttt
    33181 cgcgtttttc ctgtaagccg tggcgggcta aaaatgcgtc atcctggagt agcttattaa
    33241 gtgcatcgcg gtattgagcg aatttcggat ggtcagatgc gtccatcata ttgtcgatga
    33301 actgctgcat cggcggttgc ttaatacctt cgaggttgta accgacgctc atattaaaga
    33361 tgaacgattt acaggaatca gaaggctgga acatcgcttc gagaaggtgc agggcaaacc
    33421 aggctttgag gtattcatcc caggctttaa gcagggtaaa ttcggtagac cattcggtgt
    33481 taaagcactc gtcttcggca tcgatacagg gcttttccag ctccaggcgg tcaagaattt
    33541 ggacggtttt tagttcgatg aagcgtccgc cagtcagcca ggaagtgaca atattttgcg
    33601 cgagctgcgt gtgcgggcca gcggcagggc cgacgggagt ggcacaggtt tcaccgaaga
    33661 cgctaacagt tttacctttt acgggtgagt aaaactgttg ctcgggaata ccaaagattg
    33721 agcgttgttg ttggtattca tcaaatatgc gcgtcaaaag ttcctcaaac ggaatgggac
    33781 gcataatatc ccccataacc ctctccttag cttatacaga tatcattgag tgaccggtgt
    33841 tgagaaatcc ggtggtttag ggggatagag caacaatcgc accactcttg ccaggtttgg
    33901 cgggaaatat aaaaaaatgt gaggcggctc gaaacttaat ggctttttgt gaatgaactc
    33961 acaggtgcca aatgagaatt aaaatcactc ctgaaaatta ttattgccag cccatttatc
    34021 actttttctc ttttctcaaa atcagtgatt ccttatcatt atgataaaga gtgctggagg
    34081 gattaaatat ttgaacgcaa tcgtttaact acgttttcta aatttcagta taccttttct
    34141 tcgctgtaat aaagtcgtca ggagtatcaa tatctaaaat tatttctgca ttttctattt
    34201 cgacggaata atgttctccc ataaggagtg cctgacgcat attggtgaca ttgggtcgct
    34261 ggattgcctg catcaggcat ggttttgaca ctaaaatcgg atggccgggg atgccattat
    34321 ggagtggcag tattgcgcca tcatttcgta acgaccagat ttttctaaaa atatcgatgg
    34381 tgagggttgg catatctccg tgggtgagaa aacaatgttc tgtttgcacc gcgggtactg
    34441 cggcctttac tgatgtcagt aaaccctgcg cataatctgg gttgtggata atagtgatat
    34501 tgctctggtt cgcataacgt tcgtgcagtt cattaccacg atagccggtg actaaaataa
    34561 ttcggctaca aaactgcaac gcatttttga tacttgtatc aagaattgtt ccctgttgcc
    34621 agggtaacat aattttccat tgccccattc ttgatgataa tcctgcggcg gtaattatac
    34681 agtcgatggc tgacatagtg attccattat ttacaggagc taataacgcc tgataattgc
    34741 gttacttcct gatggattaa gttgagggta atttatccca gagaggtcca taaagacgcg
    34801 tgaaaagtat aattgaccca tcggcattat tcattgattt aggcgcgcag aaacgtccca
    34861 ctgtgatttc tgttgttggt gctgggggga aaaccagctt gcttttttgg ctggcagaac
    34921 tgttccaggc gagcggcagg cgtgtattaa tcactacgac gacacatatg tttatgccaa
    34981 catctcactg gcccgtggtt ttctgtcgtg atcccgccat gcttcctcat gcgtctttta
    35041 tatcccccat ttcattttgt tttcacagct ggaaagcgaa ccagggaaaa gtgcagggat
    35101 ttacgccaga agcgattgat gcactggtac aacgatcaga gtgtgacgta attctcattg
    35161 aggcagatgg ctcgcgtgga atgccgttaa aagcgcctga tgagcacgaa ccttgcatac
    35221 ctaaaagcag ttgctgcgtg attgctgtga tgggaggaca tattttgggt gcgaaagtga
    35281 gcacagaaaa tgtccatcgc tggtcgcagt ttgctgatat taccgggtta acacctgatg
    35341 caaccttgca actgagcgat ctcgttgcgc tggttcgcca ccctcagggg gcgtttaaaa
    35401 acgtaccgca aggttgtcgg cgagtctggt tcattaaccg tttttctcaa tgtgagaatg
    35461 cgattgcgca aagcgagctc cttcaaccgc tgcaacaaca cgacgtagag gcaatctggc
    35521 tgggcgatat acaagaacat cctgcaatcg cgcgcagatt tgtgaattag cggcccgggt
    35581 attccgggtt actgaaagga tatccattga ggttggtatg aatattttca cagaggctgc
    35641 aaaactcgaa gagcaaaatt gtccgtttgc gatggcgcaa attgttgata gccgaggctc
    35701 aaccccccgc cattctgcac aaatgttagt gcgcgccgat ggctctatcg tcggtacaat
    35761 tggtggcgga atggttgagc ggaaggtgat tgaagagtcg cttcaggcat tgcaggaacg
    35821 taagccgcga ttattccatg gacgtatggc tcgtaacggt gcggatgctg tcgggtcaga
    35881 ttgtggaggt gcaatgtcag tatttatcag cgtccatggt atgcgtccac gtctggtgtt
    35941 gattggcgcg gggcatgtca accgggcgat agcccagagt gcggcgctat taggatttga
    36001 tatcgccgtt gccgatattt atcgcgaaag cctcaatcct gaactattcc caccatcaac
    36061 cacgcttctc catgctgagt cgtttggtgc ggcagtggaa gcactggata ttcgccctga
    36121 taattttgtc ctgattgcca cgaataatca ggatcgtgaa gccctcgaca aactcattga
    36181 acaacccatt gcatggctgg ggttgctggc aagtcgccgc aaggttcagc ttttcctgcg
    36241 ccaattgcgt gagaaaggcg tggctgaaga acatattgcc cgtttacatg cgcccgttgg
    36301 ttacaacata ggtgcggaaa cgccgcagga gatcgccatc agcgtgctgg cagaaatatt
    36361 acaggtgaaa aataacgcgc cgggtgggct gatgatgaag ccttctcatc cttccggaca
    36421 ccagctggtg gtgattcgtg gtgcggggga tatcgccagt ggtgtagcgc tacgtctgta
    36481 tcatgcgggt tttaaagtga tcatgctgga agtggaaaaa ccgacagtga ttcgttgtac
    36541 cgtggcattt gcccaggccg tgttcgatgg cgaaatgacg gtcgaaggcg tcaccgcccg
    36601 cctggcaacc agctctgcgg aggcgatgaa acttaccgaa tgcggattca tccctgtgat
    36661 agtagatccc gcctgttcat tgcttgatga actgaaaccg ctttgcgtgg tggacgctat
    36721 tctggcgaaa cagaatttgg ggacgcgggc agatatggca ccagtaacaa tcgcgcttgg
    36781 gccgggcttt actgcaggga aggattgtca tgcggtaatt gaaacaaatc gcgggcactg
    36841 gctcggtcag gtgatttact ctggttgtgc gcaggagaat accggtgttc ctggcaatat
    36901 tatggggcat accacccgac gggtgatccg tgctcctgct gcaggcatta tgcgatccaa
    36961 cgtgaaatta ggcgatctgg tgaaagaggg cgatgtgatt gcctggattg gtgagcatga
    37021 aattaaagca ccgttgacgg ggatggtgcg tggcttgttg aacgacggac tggcagtggt
    37081 cggtggtttt aaaattggtg atatcgatcc tcgtggtgaa acggctgatt tcaccagcgt
    37141 ttctgataaa gcccgggcga ttggcggcgg cgtacttgag gcgttaatga tgttgatgca
    37201 tcagggcgtt aaagcgacga aagaagtgct ggaagtggct taaatgaaaa tggccggatg
    37261 gtattaccca cccggccttt aaattactga caaatcaccg ttccggtttt gccttcaatc
    37321 ccttctttcg ctttgctcaa tacggtaatc agtgcttcac gacctgcgcg agagcgagca
    37381 aacgaggcgg cagcttccac tttaggcagc atagaacctt tcgcaaaatg accttcttcg
    37441 ataaagcgtt cagcatcact taatgacagg cgatcgagcc actgttcatt ctctttaccg
    37501 aagttaatgg ccactttttc taccgcagtc agaatgatca gcatatcggc atcaatcatt
    37561 tctgctaaac gggcgctggc ccagtcttta tcgataaccg cactggcacc gcgcagatgg
    37621 ttcccttcac gaataacggg aatgccacca ccgccaacgg tgatcactac ctggcctgct
    37681 tctaccagcg cttttaccgt ctctttttca ataatatcaa ccggtttcgg cgaggcaaca
    37741 acacggcgat agccgcgacc agcatcttct ttcagagtgt aaccctgttt tgtcagttgc
    37801 tctgcttcct gctcggtaaa gaacgaaccg atcggcttgg tcgggttgag gaatgccggg
    37861 tcgttagcat cgacttcgac ctgggtaacc agcgttgcta caggtttatt aatgccacga
    37921 gaaagcagtt cttctcttaa tgcgttttgt agatcgtaac caatataacc ctggcttaag
    37981 gcaacacaga cagacatcgg cagcattggc gagtgcgctt cagtttttgc ggcagcttca
    38041 aaggcctggt taatcatgcc cacctgtggc ccgttaccgt gagtgacgat aacttcatgt
    38101 ccctgggcaa ttaaatcaac aattgcctga gaagtaattt ttaccgcttt catttgtccg
    38161 gccaggtcat cgcccagcgc attcccgccc agggcgagaa caattttctt actcattttt
    38221 cctcactcat cattcttgat tgtgtataga tccctggcag tggttagagc acgggaggga
    38281 caaacggttt gcgtcgcagg aaacgacctc gcccggcttt gcctgtaaag gtgccgtcac
    38341 agaaaatcgt ttcaccacgg gataaagttc tgacaatcgc gccctgacag gtaaaaccct
    38401 cccatggcga gtagtcggcg ttgtcgtgga gatggcgatg ctgaatttgt tgactctgac
    38461 gtgggtcgat aatcaccacg tcgccatcgg aaccgggcgc taataatcct ttttgcggcc
    38521 acaggccaaa cagcctggcg ggcatcgcgc tggttaattc aacaaaacgt tccggtgtta
    38581 tacgtcccgt cataacgcca ctggaaaaca gtaactgcat gcggttttcc acaccgggta
    38641 agccatttgg gcagcgactg aaatcgcctt tagaaatttg caggcgttga gccatcgaga
    38701 atgtgcagtg atcggtcgct accacgtcaa tcgcaccatc gctgatgcca caccacagtt
    38761 tgtcctgctc gcgtacgtta cgcagcggtg ggctaagaat gaacttcatg ccatcttctg
    38821 tatcgtaact gcgttcgtcc aacaggagat attgtgggca ggtttcaacc cagactggct
    38881 ggtgattcgc acgggcaaga cgcagataat ccagacctaa gccgttagac aggtgcacga
    38941 tatagagcgg ggcgttaccg gcaatttgtg ccaggttaat catgcgggcg atggcttccg
    39001 cttcgcattc cagaggtcga ctcaaggcat gataacgcgg cgcggttaac cctgcggcga
    39061 taaactccgc ccgcttgctg gcgatagctg cgtcattttc cgggtgcacg gtggtcagcg
    39121 cgccggattc atgcaagcgg cgtaatgcct gcaacacttc atcatcgttg agtttatatt
    39181 gataggttaa atagagttta aaactgctca gtccttcctc aaccatcatc ggaatttcgt
    39241 cgaggatagc gtgattgata tgttgaatga caccgtgaaa gctgtagtcg atgactgctt
    39301 tgtgggcggc ataaccgcga taaacttcca gttgatggcg taaccggcag ccattcgggc
    39361 caaatcccat atggtcaata atggttgttg taccgccaca cgcagctgcg cgggtaccgg
    39421 taaaaaaatc atcacaactg cgcgcgatgc cgacatcaat attgaaatgc gtatggacat
    39481 ccacgccgcc agggaaaacg taacagccag tggcatcaat ttcttcatac gggacctgcg
    39541 gcgaaatatt gctgcccaat tggcgaacaa tcccgctctc aatcagcaaa tcttgtttgg
    39601 cttgtccatc tgcgttaacg acagtgccgt ttttgatcaa tacgcgcata gcaaactcca
    39661 aaaaccctcc ggcgaaccgg aggggaataa gtgaagagta attattcggt tgccagccag
    39721 cttaacggga ttgcagcgta catcgcggca caggtcacca ggtgagattt ccaggttttt
    39781 tcgttaggtg catgcgcttc aggttcttta cccgggccaa agccgatcac cggaatgccg
    39841 tgacggccca tgatagatac gccgttagtt gagaaggtcc acttatcaac aaccggcgct
    39901 ttgccaaaca gaccttcgta ggcattcacc agtgctttaa cggtgaagtg atcttcttcc
    39961 actttccagg tcgggaagta gcattcggtt gggtaaacca ggccagtcca ggacggacgg
    40021 tcgtagttgt acatagaaac aaccgcgtta gctttctgta ctgcaggcag ggcgcggatt
    40081 tcgtccagcg cgccttccca ggtttcgccc caggtcagac ggcggtcaat agagacggcg
    40141 cagctatctg ctacagcgca acggcttggg gatgtgaaga agatttcaga aacggtgagg
    40201 gtgcctttgc cgaggaattc gtcataaccc agacgttggg agagttcttg taattcgcca
    40261 agaatcggac ccattttgaa aatggcgttg tcaccgcgtt ctggtgcaga accgtggcag
    40321 ctcacaccct gaacatcaat acgaatttcc atacgaccgc gctgaccacg gtatacctgg
    40381 cagtcggttg gttcggtact gaccacaaat tccgggcgaa tgccggattg ttcaataatg
    40441 tactgccagc acaggccgtc gcagtcttct tcctgcacag taccggtaac cagcagggta
    40501 tattcatctt ccagaccgag gtctttaatg attttaccgg cataaaccat agatgccatg
    40561 ccgccttcct ggtcggaagt accgcgacca ccgatcagct catcagtttc catgccttcg
    40621 tacggatcga agtcccagtt tttgatgttg ccaatgccga cggtatcgat atgagcgtcc
    40681 attgccacca gacgcgggcc gtggccgata taaccgagaa cgttgcccat cgggtcgatt
    40741 tcaactttat cgaagccgac tttttccatc tcttctttaa tacgatgtac tacgcgtttc
    40801 tcgtcgcagc tttcactggg aatagcaacc atatcgcgca ggaagcgagt catgtctgcc
    40861 tggtaatctt ttgctttttc aagaatcagt ttgaatggaa tattcttagc cattattttt
    40921 ctccagattt tttactcccc gttttgcagg gagcagttcc aatagggtga ttaaggtgct
    40981 acagcgtgtt tgccttccca gacaacttcg cggtagtgct tcacgtcggt gtcgccttca
    41041 gtgctgataa ccagcaccac ggcatctttg ttcagcgcca gtttttccat caggctttga
    41101 cgttgcgggt gataatgaac cgctgcgaga acgcccaaac cgacagcgcc ggattcaccg
    41161 gagatgatgc gcgggtcgtt gccatacgga ttacccagca cgcgcatacc taatgcggca
    41221 acgctgtcct ggcaggagat gaattgggtg gcgcagttac gtaggatttc ccagcccagt
    41281 gggttaggtt cgccgcaggc caggcccgcc atgatggtgg ccatatcacc gccaacgttg
    41341 acgatgtcgc ctttgacgcc ggagcgataa atacagtcag ctttgtcagg ttcaacaata
    41401 atgctgtgca gattttgcgg gctatagacg tcgaccagat aacccagcac accaccggcc
    41461 attgcaccga caccggcttg cagcagaacg tgtgtcgggg tgacgcccat ttcacgcatt
    41521 tgctcgacgg cttcatccgc cagtgttgcg tagccttgca tgatccaggt tgggattttg
    41581 gtgtaacctt cccatgccgt gtcctgtacc acttcccagc cgtgctgctg cgcgtgttgc
    41641 atggtcaggc gaacggtatc gtcatagttc atatccgtga cgatgcactc ggcaccgagg
    41701 tttagaatgg cgtcaacgcg ttcctgagca gaacctttcg gcatgtaaat caccgcattc
    41761 tgtccgagtt gctgtgctgc ccatgccaca ccgcgcccgt ggttgccgtc ggtggtagtc
    41821 gcgaaagtca ttttttcgcc gatggcattt ttcaggtgct caaatgacag cgtttcgata
    41881 tcaagatgat atttttcaca caataattga gcgatggcgt acgcaccgcc aagcattttg
    41941 aaggcgttca gaccgaatcg ttttgattcg tctttgacga gaattttctt tacaccaaac
    42001 aggtttgcga gatcgtccag cgcacaaagc ggtgttgggt gataaccagc aattttctgg
    42061 tggaactggc gtgccagttt tgcctggctt tgcgaaaaaa gcggtgatgt ttcgccgttg
    42121 aaaaatttgt tatcggcgat atcaatcttc aatgagaaaa cggacatatc ctatcctttt
    42181 ggcctctgga aatagagggc cgaccagctg atcggcccga ggggttattt cacgcgttct
    42241 tgcgcttctt tcagcagttg ctcgagcagt gcacctggtt tggcgtattt acgggacagg
    42301 atcatcgcgg cgatgatata aggcttccag ctggcttctt tgtaggtagc gatacggtat
    42361 ttttcgaata cgccttcagt cacttcacct tctttgcagg atacgccgct gatatcagct
    42421 ggcaggcagt gcatgtacag ggcttcgcca tcacgggtca gttccatcat ctcttcagta
    42481 caatgccagt ctttgtgttg cgcgttctgt gccagacact gtttttccag tgcttttaag
    42541 ccttcgtgat cgttcgcacg cagcaattca gtacgctctt ccatcacttt gtaaggtgcc
    42601 catgacttcg gataaacgat gtctgcgtct ttgaaggctt cttccatgct ggtgacctga
    42661 cggaagctac caccggaggc tttagcattg tttttagcaa cttcaacgac atccgggatc
    42721 aggtcgtagc cttccggatg ggccagggtg acatccatac cgaagcgggt catcagaccg
    42781 atgatgcctt gtggtacaga gagcggtttg ccatagcttg gggagtaggc ccaggtcatg
    42841 gcgatttttt tgcctttcag gttttccagc gaaccaaagt gttcacgcaa ccatgccagg
    42901 tcagccatag actgagtcgg gtggtcaata tcgcattgca ggttcactaa agccggacgc
    42961 tgtggcagaa ccccttgttt gtagccgtca tcaagtgccg cgccaacttc acgcatatag
    43021 gcgttgcctg cgcccagata catatcgtcg cgaataccga tggcatcggc gcagaaggag
    43081 atcatattgg cggtttcacg cacggtttcg ccgtgagcga tttgtgattt gccttcgtcg
    43141 agatcttgct gtgcaaggcc gagcaggtta agcgcggaag cataagagaa gcgggtacgg
    43201 gtggagttgt cgcggaatac ggaaatacct aatccactat taaagacttt ggttgagatg
    43261 ttttcagcac gcagtgcttt taatgctgcg gcaacgtcca gtacttgttt cagttcatct
    43321 ggcgtctgtt cccacgttaa caaaaaatct ttctcgtgaa ggtgagaggt cagcgaattg
    43381 atatccttaa tcagctcatt aacagttttc atcataaagt cccggtagtg gaatgacagg
    43441 caaagtggtt tatttcactg tgacttgcca acgtgattgg tgttttcaca gctatacctg
    43501 ggactaacaa taagtgtgcc agaattatcc acggggctgc aaaagactaa tttgacggat
    43561 aatgtgtggg gttgatcaca aattgatgcc agcttctggt tgtttgtaaa atacgaaaac
    43621 gtttggcttt ttttatcgcg ttatttattt gctgctaagt atcagaaata agataaaggg
    43681 acaggcgatc tctcttcagt ttgataaact caccgccgta attctcatat tgatatagaa
    43741 atgtgatatc tcttccataa tttcattaga aagtgataaa aatcttatgt ttttatcaaa
    43801 gccaatttcg cctgagttaa tatcatcatg atctatgaac catcaacgtc ttcaatgatc
    43861 gacttttaag taagtctgga aggtaaagct atggagcttg ctactacgca gtcagtattg
    43921 atgcaaattc aaccgacaat tcagcgtttt gccagaatgc ttgccagcgt tttgcagctt
    43981 gaggttgaga tcgttgatga aaacttgtgt cgcgtcgccg gaacgggcgc gtatgggaag
    44041 tttcttggtc gccagttgag cggcaactca cgcctgctcc gccacgtcct ggaaacgaaa
    44101 actgaaaaag ttgtgacaca gtctcgcttc gatccccttt gtgaaggttg cgatagtaaa
    44161 gaaaattgcc gcgaaaaagc atttctgggt acgcctgtca ttttacagga tcgttgtgtt
    44221 ggggtgataa gtttgattgc cgttacccac gagcaacaag aacatatcag tgataattta
    44281 cgcgaatttt ctgattacgt tcgccatata tccaccattt ttgtttcgaa acttctggag
    44341 gatcaggggc caggagataa catcagtaaa atattcgcga ccatgatcga taatatggat
    44401 cagggcgttt tagttgttga tgacgaaaat cgggttcagt ttgttaatca gactgcctta
    44461 aaaacactcg gtgttgtgca aaataatatt attgggaaac ctgttcgttt cagaccatta
    44521 acatttgaga gtaattttac ccatggacat atgcagcata ttgtttcgtg ggacgataaa
    44581 agtgaattaa tcattggtca attgcataac attcagggcc gacaattatt tttaatggcg
    44641 tttcaccaat cgcataccag tttttctgta gcaaatgcac ctgatgaacc gcatattgaa
    44701 caattggttg gcgagtgccg tgttatgcgg caattaaaac gactcattag ccgtattgca
    44761 cccagcccat ccagcgttat ggtggttggc gaaagcggca cgggtaaaga agtcgtcgcc
    44821 cgtgcaatcc ataagttgag cggaagacgg aataaacctt ttattgctat caactgtgcc
    44881 gcgattccgg agcagcttct ggagagcgaa ctgttcggtt atgttaaagg cgcatttact
    44941 ggcgcttctg ccaacggtaa aacagggttg attcaggcgg cgaatacggg cacgctgttt
    45001 ctcgatgaaa taggtgatat gccattaatg ttgcaggcta aattactgcg cgctattgag
    45061 gcacgtgaaa ttctgccgat tggtgccagt agcccaatac aagtcgacat tcgcatcatt
    45121 tctgcaacta accagaattt ggcccagttc atttccgaag gtaaattccg cgaagatctc
    45181 ttctaccgac ttaatgttat cccgataact ctgccaccgc tgcgtgaacg tcaggaagat
    45241 attgaactac tggtgcatta ctttttacat ctgcataccc gtcgtctggg atcggtttat
    45301 ccgggcattg ctcccgatgt cgtcgaaata ttgcgtaagc atcgttggcc cggaaacctg
    45361 cgcgagttaa gcaatttgat ggaatatctg gttaacgtgg ttccttcagg tgaagttatc
    45421 gacagcacgc tattgccgcc aaatctgctg aataatggca caacggagca aagtgatgta
    45481 acagaggtca gtgaggcgca cctggcactc gatgatgcgg gcggcacggc gctggaggag
    45541 atggaaaagc aaatgatccg cgaggcgctt tcacgtcata acagcaagaa ggaagttgct
    45601 gatgaactgg gcatcggcat tgctacgctc tatcgcaaga ttaagaaata tgagttgtta
    45661 aacacataac ctcagtgaat ccggcctgaa ttcaggccgg atatcctttt acttcgtttt
    45721 ctcgcaatcc agaactgtat ttacaatcat ctgatacccc gtgcagcgac aaagatttcc
    45781 cgccagtcca cgacgaattc ccgtaatggt taatggcttc tcgcgtggtt tcgccagcat
    45841 tgccgtggta gccataatca ggcccggcgt acaaaacccg cactgcactg cgccggattt
    45901 cgcataagcc tgctgaacat gagaaagttt tccgcctttc gcttcacctt ccagcgtgcg
    45961 gatctctttt ccttcagccc aggcggcaag gtataagcaa ctgtctattg ctgtgccgtc
    46021 gaccagcacc gtacaggcac cacattcacc cacgcagcac ccttgtttga cacttagcag
    46081 tccttgttcg cggagtaatt ccgagagcgg cgtgcctggt gcggcgtgaa gctgaaaagg
    46141 tatcccgtta atggtgcatt cgatggtaat tgtttcgctg tgattcattg caattttccc
    46201 cccgccgcgg cgacggcttc gctaatcact tttttggtca tcgtctggat gagatgcaga
    46261 cgaaactctt tactggcccg ccatgacgaa cgcggggcga catcttgcag gactgattcg
    46321 ctgatggctt ccagcgtttg caggtttaat ggcgcatttt gtgcagtctg ttcggcatgt
    46381 tggcagcgaa ttggcgttgg cgcggcaaca ccaaatgcca ggcgtaattc gctgaaattg
    46441 ccgttatcca gttggcaatg tgcggcgcag ccaatcgttg aaatatccat tgcgtcgcgc
    46501 atggcatatt taaaatgcgc gctgcccgcg tgttctttcg gctggggcgg aaaatgaaag
    46561 gcgacaagga tttcgtcatg ctcaagagac actttgcccg gcccggtgtg aaagccatta
    46621 atcgggacga aacgaacacc gcgcggggag tggatctcca gtttcgcgtc ataaattagc
    46681 gttggcgtgg cagaatctgc gctggtggca ccgttgcaaa tatttccacc gtaggtagcg
    46741 acgttacgga tctgcggtcc ggcaatggat gaggccgcag cacataacgc cgggagatga
    46801 cgttgagtta caggatcttc aatgagctgg gtaaatgtcg ttgcagagcc gatacgtagt
    46861 gagccatctt ccgccagcgt aattccccgc agctccgcca gattatgaat atcaacaata
    46921 tggcgataac ggtcattgtg atggtggagc tgaatcagta cgtcagtgcc accggcgagc
    46981 agtttggcct gcgggttgtc agccagcagg ttgatggcat cggcaagggt tgctgcgcga
    47041 tggtaagaag caaaatcaaa catgatgtta tcctcaaatc aatcctgcca gatggaactc
    47101 ttcatataac cgttttggcg tcagcggcag tgtattgatt gcaacaccgg tagccatctt
    47161 caccgcgtta cgaatagcag cggcaacagg aattattggt ggctcaccca gtgacttatg
    47221 tccgtatgcg gattgcggct cattgatttc gacgaacgcg ctttccagtt gtggcagatc
    47281 cggcatggtc ggcattttgt aatccagcag attggggtta cggaccacgc cgcttttcgc
    47341 atcaatgatc atctcttcaa atagcgccca gccaatgccc attcccattc cgccgtgtac
    47401 ctgaccttct gccagtagcg gattaagaat atggcccgaa tcatgaacgt tgaggatgcg
    47461 gttgatggtg actttacaca gcgcaatatc gaccgtcaga tcaacaaagg tacagccaaa
    47521 cgctggtggg ttagtggtgg ttttgatgga gctttcggca gagagctgcc cgccgcgttc
    47581 agggtggtag aaggcgtcca tcgccaaatc ttttaacgac attaatggct cttccggtcg
    47641 ttcaaccagc acgatatggc cttttatcag ggtaagattc atcgctgact gatgtagcat
    47701 gacagcggca tgagcgatga ttttctcctt taataacagt gccgcactgc gcagcgcagg
    47761 cgcggcaaca tagctctggc gtgaggcaaa tgcaccggga tcgaacggcg taacgtcggt
    47821 atcttgggtt gaaataacgc gaacgtcgct gaccggaacc cccacggttt ctgctaccat
    47881 ttgcgagaag acggtgtcgg ccccctgacc gatttctgtc gcgccgcttt gcacgttgat
    47941 ggttccatcc tgattcatca acaggcgcgc gcctgctatt tctacgccga caggccaggt
    48001 gttagaggtg tagctaaaac aggcgacgcc aacgccgcgg cgtaaattgc cttgctggtt
    48061 ctggcactct gcacggcgtt tttcccattc aaagattttc cggccttttt caagacactc
    48121 cggcaaccct gcgctgtaaa tacgtttgcc cgtgagcgga ttagcatctc cttcgcgggc
    48181 ggcgttgcgt aaacgaattt caacaggatc aatacctaac gctgtcgcgg cgtcatcaag
    48241 catagactca acggcaaata cgacttgtgg cgcgccataa ccacgcatcg caccagccga
    48301 ggggaggttg gtatagcagg tctttgaact gtaagcgtag gcacaacgag gataaaggta
    48361 agcgacttta tttccgcccg cagaagcgat ggagtgcccg tgagatgcat aagcgccggt
    48421 gttagacaga acatccagac tataaccttt caatgttccg tcgcggttca cgcccatttg
    48481 cccgtcaata gtaaaagcgt ggcgggtacg ggttgcgagg aaacactctt cacggctaag
    48541 ggaaactttc accggaatgc cgccaagctt gctggtcagg aatgccgcca ttggctcttc
    48601 cagtacatcc tgtttattac caaaaccgcc accgacaaac ggtttgatga ctcgtacgca
    48661 tgaccaggga atattcagcg cctgaccaac cacacggcga acaatgtgcg ggatctgggt
    48721 gctggaaacg atggtaattc gcgagtcatc ctccatccat gccagcgatg tcacgctttc
    48781 catatgacaa tgttgaataa cgggagtctg atagtgcccc tgcacctggt aatcggcggc
    48841 atcgattgtt tgttggacat tacccgtcga catcgagctt tgttttagta aattgccgcc
    48901 gttatggatt ggtgcagcgt catctgccag cgccgcttct ggcgtggtga taacgggtaa
    48961 ttcttgccac tcaatgctga ccaattgcgc cgctttttct gccgtgagtt catcacgggc
    49021 cacgacgatg gcaacggcgt caccatgatg acgaacatgg cgagttagca gtgcgcgatc
    49081 ggcggtatcg cgcttgtttt cgtcaagtgt ccaggcatgc ccagctgtag cgaatgggat
    49141 atcaggcaca tcttcccagg taaaaatcgc cagtacgccc ggtaaacttc tggcttgttc
    49201 atcattaata cttatggcat aaccatgtgc gatagggcta cgtacatatt tcgcgtaaca
    49261 catgcccgcc ataacataat cgtcagtata tcgtgcccgc ccggtgacct tagcaatggc
    49321 atctacgcgc atgcatgatt cacctgtagc ggttgcttcc cgcgcttcca tagaaatccc
    49381 ctcgatttga acatttggtt cttatggcaa agatttagtc taagcaggca agatttacgc
    49441 cagaaacgac gataagcgtt aagggaggaa ggaagtgtga tgatgttaaa aaaacacaga
    49501 tatgatacga aacaggaccc tgaatagggg cagtgatacg tatcaatatg agtggcgata
    49561 aaatcaatgt gataaattgg gattattacg catcaggatg ggaaataggg taaatgagtc
    49621 agaaaattcg atacatctac agtgaatatt ttgctgatga tacttccctt tgccaaatga
    49681 aattaacctc tttctctatt accgcgtttt tccagaagca agagattggg tgtcaagagg
    49741 ctggctgcta tgatatggcg tcttgttttt taagcgagga aagattttga gtgcgggacg
    49801 cctgaataaa aaatctctgg gtatcgtgat gttgttatcg attggactgc ttttggcggg
    49861 ctgctcgggt agcaaatcat ccgatacagg aacgtattcc ggctccgttt acaccgtgaa
    49921 acggggggat acgctatatc gtatttcgcg caccacggga actagcgtaa aagagctggc
    49981 gcgactgaac ggcatttccc ccccttacac cattgaagtt ggtcagaaac taaaactggg
    50041 tggggcgaaa aatagtagta cacgtaaatc aaccgccaaa tcaacgacca aaaccgcatc
    50101 ggttacaccg tcatcagcag taccgaaatc ttcctggccg ccagtagggc aacgttgttg
    50161 gttatggcca acgacaggga aagttatcat gccgtattcg acagctgatg gcggcaataa
    50221 agggattgat atctcagctc cacggggtac acctatttac gccgcgggtg caggaaaggt
    50281 ggtgtatgtg ggcaaccagc tgcgtggcta cggtaatctc atcatgatta aacacagtga
    50341 agattacatt acggcttacg cccataatga cacgatgctg gtaaataatg ggcaaagcgt
    50401 gaaggctggg caaaaaatcg ccactatggg gagcactgat gcggcatcgg ttcgcctgca
    50461 tttccagatt cgttaccgtg caacggcaat tgatccgcta cgttacttgc cgcctcaggg
    50521 cagcaagcca aaatgctgat ggcgaattaa tcagcagtca gcagcgcggc acttgctaag
    50581 gagagcgtaa ggtttataat gccttacgca tctcgaagcg ggcgtagttc aatggtagaa
    50641 cgagagcttc ccaagctcta tacgagggtt cgattccctt cgcccgctcc aaacaaacat
    50701 ctaccaacgt ctactaaagt ctgccaatcc cccgaatatc aaggcctcca gcgattttcc
    50761 tcgtctacaa gcgtctacag tcatctacct aaatctacac acagaagtgt atagtaatgc
    50821 gtataggaca aaatcgtttt ttgggggcta tacacatgtt gctttcagac cttgctatcc
    50881 gccgcgcgaa gccaaaagag aaagcctata cactcaatga cggcaacggg ctttcgttac
    50941 tcattgagcc taacggttcg aagggctggc gattgcgata caggtttgca ggtaagccca
    51001 aaatgttgtc gctgggtgtt tatcctgagg tgtccctttc tgatgccagg gcggcaaggg
    51061 atgaagccaa aaggcttatc gctggtggag ttaatccaag tgatgtgaga aaagcccata
    51121 agagagaaca ggcgagcaaa ttcggtaaca cctttgaggc gatcgcccgt gagtggtatg
    51181 aaaaacgcct ggacagatgg tcacaatcat atgccgcaga gatgattgat acgtttgaga
    51241 aggacgtatt cccgtatatc ggcagcaaac cgatagcaga aataaaaccg atggaattgc
    51301 tcgcggttct ctcccgtctt aatgaccgtg gcgcaactga aaaattgcgt aaggttcgcc
    51361 agcgatgtgg cgaagtgttc cggtacgcaa taattaccgg acgcgctgaa tataaccccg
    51421 ctcctgatct ggcaagcgca tttgcaccac acaaaaaaga gcattacgcg tatctgaaag
    51481 ttgatgagct accggaattt ttccgcgttt ttaatacgta caccggaagc cagatcgtca
    51541 agctgggtat gcgattattg attcttacag gactccgccc aggtgaattg cggcaggcag
    51601 agtggcagga gattgatttt gataatcgtc tgtgggaagt gccgaaagaa cgaatgaaaa
    51661 tgcgccgtcc tcattgcgtg ccgctttcag aacaggcgat agctattctg gaacaactga
    51721 aacacatgac ggggcagtat ccgtttattt tccctggtcg aatacagcac agtaaaccga
    51781 tgagtgaaat ggcgatgaac gtgcttattc gtcgtattgg ttatgcaggg cgtgttactg
    51841 gtcatgggtt ccggcacacc atgagtacga ttttgcacga gcaggggtat aactccgcat
    51901 ggatagaaac gcagctggcg cacgtcgata aaaactcgat ccgtggcaca tacaatcacg
    51961 cgcaatatct ggacggacgg cgggagatgc tccagtggta cgccgactat atggactcac
    52021 tggaacaggg cggaaacgtg gtacatggca agtttggaaa atgtggatga ctggttgagt
    52081 atacagtagt agactttgag cgactaaata acaggctgtg tctagggtcg ctcccgaaac
    52141 cccgtacacc tctgcggact ggcacagcca ccactacaga gagtgcgagg tgtgcacatg
    52201 ccggttgtga atgatttccc tgacttaagt cattggttta cagtgcagga gtttactatt
    52261 actcaagctg cgttattgct gtcaggtatt gatcctctcg actacccaac tctggagatg
    52321 gtcaaagagg aaaaacacga acgatggaga atggcatatg ggtttgctta tggaatggtt
    52381 actgcaattc gacgcggtgt cttaactccc gttctgtgcg ttgcgtatga ttggggggat
    52441 atgcgtcgtt taaccccagc agagatgatg gatcgttcaa ataatatatc catggacgga
    52501 acaattatta cccgttattc attacaggaa tgggtaaaaa atgagaaagt tgattatgta
    52561 agaccctctc gctctatatc ggataaggag attaagccat ctaaccaatc agtaataatt
    52621 gatgccgaat ctagcgtgat taattctgaa gaaacagtat tgctgactta tggtcataag
    52681 agcgaaggtc ttgaatatgt agaagacgca ataagagaac tttgggcaac atatgatcca
    52741 gataatcaac aaacagcccc tacaagaaat gatgttgttg aatacctgaa aggcagggga
    52801 gcggggcaga acatggccga ggctgtaaac ctgatactac gccccactgg cttgcagcaa
    52861 gttggaagaa ggaaaagaaa aacaaatagt taaatagtgc ccacccttcg taaaaaaggt
    52921 ggcactattt gctcaaaact cttttgttta tggcgagaaa tcatagttaa gcgtgcgttt
    52981 tctttgctta ttgatactgg aatggtggca gcattattaa aataatagtg ggcactattc
    53041 cattgaaata gtacccacca ttaaaaatag tatccaccta taaatatgtt ttccttttat
    53101 gcatcattgt agacggttat agacgttacg aggattgaat atgtcaaata acttacttcg
    53161 tctttctgat gttatgcgcc gtactggtta cggtcgcgct tggatttatc gcctgatcaa
    53221 ccaggggcgc tttcctaaac ctgtaaaaat tggtccccgc tccgtggctt ttattgaaag
    53281 cgaagtcgat gaatggatta accagcgtat cgaggcttct cgcaacaacg ccgcgtaata
    53341 atcttttggg agggggatat tctcccccag cactaaacct gatccgatag caggagtttt
    53401 tcaatgaaac gtccgaatat gcccgtacag gggcagggct tcgttcagcc cgaagaactg
    53461 agccaacagt atttcgccgc aattattccg gtaatttccg gcaatgagat tattgctgac
    53521 ggaataaata aaaatgcact caccgcgccg gaagtgtttc cttctggcgg tgagcctgta
    53581 accacttcac aggctatcgc ccgatttttc agaaaggatc acgataaggt tgttaaccgg
    53641 attgaaaacc tgtgttgttc tgctgaattt ttaacccgca attttcggcg ggtaaaattt
    53701 gagcataagg ggcgcgtatt gaaagcctac gaagtcacca aagacggctt cgtgttttta
    53761 acaatgggat ttaccggaaa aaaagcggct gcattcaaag aggcatacat tgcgcagttt
    53821 aaccgcatgg ctaactggat agcagagaga tgtgaggcca aagctgagat gcgctctatg
    53881 ggggatgcca tgaagggata cattgagcgc actggagata ctcaacacgg tcatgcttat
    53941 aacaacgaat gtactttcat tgcgcgcctt gtattaggga tgcacccgcc tgtatgggcc
    54001 aaaaaaaacg gcatcccatc aaaaaaagtt cgtgaccata tgaccgctga tcaactggcg
    54061 ttgctcgcgt atctggaaag ccgtaactgt gcgctactgg atatggatat gccaacgcca
    54121 gaacgtaaag aacgtctcac cgaattagcc caacgctggc tggtccgtcg cctgggagaa
    54181 cgtgaacatg cctgacatcc tcaaacctgc tgtccggtac atcgacacgg cggataagtt
    54241 cgcactactg aacatcaacc atgcggatta catgcgccat atcgtgagcc atttcaccgc
    54301 tgagcgcatg aaacacgtta agcaatactt ggaggagggt ttatcaaatt atcgcgctgg
    54361 tggccctgta cggcgcgaat atccgcatga tgcaggaatg gcaggcaaaa caaaggcgac
    54421 cacgaaaggg tcgccagtgg agataaagga aatcaagcgc attatccaca atgccatatc
    54481 tga
//
LOCUS       DAZKGF010000010        49753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZKGF010000010 DAZKGF010000000
VERSION     DAZKGF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.010"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            172..798
                     /locus_tag="ACHOQV_000606"
     CDS             172..798
                     /locus_tag="ACHOQV_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFP9629029.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTHRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            819..2921
                     /locus_tag="ACHOQV_000607"
     CDS             819..2921
                     /locus_tag="ACHOQV_000607"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFP9629030.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRKTLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2934..3572
                     /locus_tag="ACHOQV_000608"
     CDS             2934..3572
                     /locus_tag="ACHOQV_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFP9629031.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDHADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDIAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3636..4304
                     /locus_tag="ACHOQV_000609"
     CDS             3636..4304
                     /locus_tag="ACHOQV_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9629032.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTMQEKKVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4301..5086
                     /gene="phsC"
                     /locus_tag="ACHOQV_000610"
     CDS             4301..5086
                     /gene="phsC"
                     /locus_tag="ACHOQV_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFP9629033.1"
                     /translation="MNPSQHAEQFQSQLANYVPLFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSANDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     TVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDMFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5090..5902
                     /gene="ydhT"
                     /locus_tag="ACHOQV_000611"
     CDS             5090..5902
                     /gene="ydhT"
                     /locus_tag="ACHOQV_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFP9629034.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGEDSTIVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5908..7518)
                     /locus_tag="ACHOQV_000612"
     CDS             complement(5908..7518)
                     /locus_tag="ACHOQV_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFP9629035.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEEIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAIAQAVVKPASRARRRLSFNQM"
     gene            complement(7644..7949)
                     /locus_tag="ACHOQV_000613"
     CDS             complement(7644..7949)
                     /locus_tag="ACHOQV_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFP9629036.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTNEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8057..8133)
                     /locus_tag="ACHOQV_000614"
     tRNA            complement(8057..8133)
                     /locus_tag="ACHOQV_000614"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8097..8099),aa:Val,seq:gac)
     gene            complement(8138..8214)
                     /locus_tag="ACHOQV_000615"
     tRNA            complement(8138..8214)
                     /locus_tag="ACHOQV_000615"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8178..8180),aa:Val,seq:gac)
     gene            8523..9779
                     /locus_tag="ACHOQV_000616"
     CDS             8523..9779
                     /locus_tag="ACHOQV_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP9629037.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSTYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGSINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9820..11193)
                     /gene="mdtK"
                     /locus_tag="ACHOQV_000617"
     CDS             complement(9820..11193)
                     /gene="mdtK"
                     /locus_tag="ACHOQV_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFP9629038.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            11408..12049
                     /gene="ribE"
                     /locus_tag="ACHOQV_000618"
     CDS             11408..12049
                     /gene="ribE"
                     /locus_tag="ACHOQV_000618"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFP9629039.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12089..13237)
                     /gene="cfa"
                     /locus_tag="ACHOQV_000619"
     CDS             complement(12089..13237)
                     /gene="cfa"
                     /locus_tag="ACHOQV_000619"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFP9629040.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13528..14739)
                     /gene="punC"
                     /locus_tag="ACHOQV_000620"
     CDS             complement(13528..14739)
                     /gene="punC"
                     /locus_tag="ACHOQV_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFP9629041.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
     gene            14852..15784
                     /gene="punR"
                     /locus_tag="ACHOQV_000621"
     CDS             14852..15784
                     /gene="punR"
                     /locus_tag="ACHOQV_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFP9629042.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15781..16806)
                     /gene="purR"
                     /locus_tag="ACHOQV_000622"
     CDS             complement(15781..16806)
                     /gene="purR"
                     /locus_tag="ACHOQV_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFP9629043.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17105..17194
                     /gene="cydH"
                     /locus_tag="ACHOQV_000623"
                     /gene_synonym="ynhF"
     CDS             17105..17194
                     /gene="cydH"
                     /locus_tag="ACHOQV_000623"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFP9629044.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVVVGLTAALH"
     gene            17360..18529
                     /locus_tag="ACHOQV_000624"
     CDS             17360..18529
                     /locus_tag="ACHOQV_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9629045.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18675..19256)
                     /gene="sodB"
                     /locus_tag="ACHOQV_000625"
     CDS             complement(18675..19256)
                     /gene="sodB"
                     /locus_tag="ACHOQV_000625"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFP9629046.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19384..20211)
                     /gene="mepH"
                     /locus_tag="ACHOQV_000626"
     CDS             complement(19384..20211)
                     /gene="mepH"
                     /locus_tag="ACHOQV_000626"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFP9629047.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASANFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20546..20893
                     /gene="grxD"
                     /locus_tag="ACHOQV_000627"
     CDS             20546..20893
                     /gene="grxD"
                     /locus_tag="ACHOQV_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFP9629048.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20944..25560)
                     /locus_tag="ACHOQV_000628"
     CDS             complement(20944..25560)
                     /locus_tag="ACHOQV_000628"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP9629049.1"
                     /translation="MADNLNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSMERLDTLLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQADEWYSRVRRAAPWKDLPRRVFDVTLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARTLLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFAASLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLAVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTSEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEANQANSSALQQAIVTVLADGGAWFAQQISQRIRDKIGESVDPSAMQEAL
                     WALVWQGVITSDIWAPLRALTRSSTNTRTSTRRSHRARRGRPVYAQPVSPLVSYNTPK
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANAWGNL
                     LPWPAHPATLIPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEIFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25653..26300)
                     /gene="rnt"
                     /locus_tag="ACHOQV_000629"
     CDS             complement(25653..26300)
                     /gene="rnt"
                     /locus_tag="ACHOQV_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFP9629050.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26403..26810)
                     /gene="gloA"
                     /locus_tag="ACHOQV_000630"
     CDS             complement(26403..26810)
                     /gene="gloA"
                     /locus_tag="ACHOQV_000630"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP9629051.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26891..27988)
                     /gene="nemA"
                     /locus_tag="ACHOQV_000631"
     CDS             complement(26891..27988)
                     /gene="nemA"
                     /locus_tag="ACHOQV_000631"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFP9629052.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28025..28624)
                     /gene="nemR"
                     /locus_tag="ACHOQV_000632"
     CDS             complement(28025..28624)
                     /gene="nemR"
                     /locus_tag="ACHOQV_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFP9629053.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKTIIATPAV"
     gene            28727..28966
                     /locus_tag="ACHOQV_000633"
     CDS             28727..28966
                     /locus_tag="ACHOQV_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFP9629054.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29015..29911
                     /locus_tag="ACHOQV_000634"
     CDS             29015..29911
                     /locus_tag="ACHOQV_000634"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP9629055.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDYLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            29992..30513
                     /gene="sodC"
                     /locus_tag="ACHOQV_000635"
     CDS             29992..30513
                     /gene="sodC"
                     /locus_tag="ACHOQV_000635"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP9629056.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30514..32526)
                     /locus_tag="ACHOQV_000636"
     CDS             complement(30514..32526)
                     /locus_tag="ACHOQV_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9629057.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMAA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPCATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELQHL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGSNALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAP
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32526..33383)
                     /locus_tag="ACHOQV_000637"
     CDS             complement(32526..33383)
                     /locus_tag="ACHOQV_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP9629058.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSNLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33386..33622)
                     /locus_tag="ACHOQV_000638"
     CDS             complement(33386..33622)
                     /locus_tag="ACHOQV_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFP9629059.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLSLAILIAW"
     gene            33823..34257
                     /gene="slyA"
                     /locus_tag="ACHOQV_000639"
     CDS             33823..34257
                     /gene="slyA"
                     /locus_tag="ACHOQV_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFP9629060.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(34304..34771)
                     /gene="slyB"
                     /locus_tag="ACHOQV_000640"
     CDS             complement(34304..34771)
                     /gene="slyB"
                     /locus_tag="ACHOQV_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFP9629061.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35045..36154
                     /gene="anmK"
                     /locus_tag="ACHOQV_000641"
     CDS             35045..36154
                     /gene="anmK"
                     /locus_tag="ACHOQV_000641"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFP9629062.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLILSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36252..36581
                     /gene="mliC"
                     /locus_tag="ACHOQV_000642"
     CDS             36252..36581
                     /gene="mliC"
                     /locus_tag="ACHOQV_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFP9629063.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLFERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36640..37296
                     /gene="pdxH"
                     /locus_tag="ACHOQV_000643"
     CDS             36640..37296
                     /gene="pdxH"
                     /locus_tag="ACHOQV_000643"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFP9629064.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37425..38699
                     /gene="tyrS"
                     /locus_tag="ACHOQV_000644"
     CDS             37425..38699
                     /gene="tyrS"
                     /locus_tag="ACHOQV_000644"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP9629065.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38758..39621
                     /gene="pdxY"
                     /locus_tag="ACHOQV_000645"
     CDS             38758..39621
                     /gene="pdxY"
                     /locus_tag="ACHOQV_000645"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFP9629066.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39665..40270)
                     /gene="gstA"
                     /locus_tag="ACHOQV_000646"
     CDS             complement(39665..40270)
                     /gene="gstA"
                     /locus_tag="ACHOQV_000646"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFP9629067.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40376..41878)
                     /gene="dtpA"
                     /locus_tag="ACHOQV_000647"
     CDS             complement(40376..41878)
                     /gene="dtpA"
                     /locus_tag="ACHOQV_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFP9629068.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42489..43124)
                     /gene="nth"
                     /locus_tag="ACHOQV_000648"
     CDS             complement(42489..43124)
                     /gene="nth"
                     /locus_tag="ACHOQV_000648"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP9629069.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43124..43819)
                     /gene="rsxE"
                     /locus_tag="ACHOQV_000649"
     CDS             complement(43124..43819)
                     /gene="rsxE"
                     /locus_tag="ACHOQV_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFP9629070.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(43823..44443)
                     /gene="rsxG"
                     /locus_tag="ACHOQV_000650"
     CDS             complement(43823..44443)
                     /gene="rsxG"
                     /locus_tag="ACHOQV_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFP9629071.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44447..45505)
                     /gene="rsxD"
                     /locus_tag="ACHOQV_000651"
     CDS             complement(44447..45505)
                     /gene="rsxD"
                     /locus_tag="ACHOQV_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFP9629072.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGMLAGV
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLASPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45506..47728)
                     /gene="rsxC"
                     /locus_tag="ACHOQV_000652"
     CDS             complement(45506..47728)
                     /gene="rsxC"
                     /locus_tag="ACHOQV_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFP9629073.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKACKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(47721..48299)
                     /gene="rsxB"
                     /locus_tag="ACHOQV_000653"
     CDS             complement(47721..48299)
                     /gene="rsxB"
                     /locus_tag="ACHOQV_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFP9629074.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISYNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48299..48880)
                     /gene="rsxA"
                     /locus_tag="ACHOQV_000654"
     CDS             complement(48299..48880)
                     /gene="rsxA"
                     /locus_tag="ACHOQV_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFP9629075.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFFQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(48957..49397)
                     /locus_tag="ACHOQV_000655"
     CDS             complement(48957..49397)
                     /locus_tag="ACHOQV_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFP9629076.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGEQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49483..49698)
                     /gene="ydgT"
                     /locus_tag="ACHOQV_000656"
     CDS             complement(49483..49698)
                     /gene="ydgT"
                     /locus_tag="ACHOQV_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFP9629077.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
BASE COUNT        12490 a        12356 c        12970 g        11937 t
ORIGIN      
        1 tttctcctaa ccataaagga ttagtttatc agcgttaaat cttttctcat acactattgg
       61 gttagggaaa atatctggtg ttgaggacag agtcagcagc gtattagttg agtgcctaat
      121 atcaggctgc tgaattgtca gcactgtgtt tgcgaagagg atgcgaaata aatgaacccg
      181 gttgatcgtc cactattaaa tattggctta acgcgattag aattcttgcg tatatcagga
      241 aaaggccttg ctggtttaac aattgcccct gcgttactgt cacttttggg ttgcaaacag
      301 gaagatattg atagcggcac agtaggcttg ataaatacac ccaaaggggt gctggtgacc
      361 catcgtgcgc gatgcacggg ttgccaccgc tgtgaaatct cttgtaccaa cttcaacgat
      421 ggctcagtag ggacattctt ctcccgtatc aaaatccatc gcaattattt ctttggcgat
      481 aacggggttg gctctggtgg cggcctgtat ggcgatctca actataccgc ggacacctgc
      541 cgtcaatgca aagagccgca atgcatgaac gtctgcccga ttggtgcgat tacctggcag
      601 cagaaagaag gctgtattac cgtcgatcat aaacgttgta ttggctgtag cgcctgtact
      661 acggcatgcc cgtggatgat ggccaccgta aataccgaaa gtaaaaaatc ctcgaaatgt
      721 gtgttatgcg gtgaatgcgc aaacgcctgc ccaacagggg cgttaaaaat tatcgagtgg
      781 aaagatatta ctgtgtgaat cttgcaaagg aaaaaatcat ggctaacggt tggacaggta
      841 atatattaag agtcaatctc acgacaggaa atattaccct cgaagattcc agtaagttta
      901 aaagttttgt cggtggcatg ggcttcggct acaaaattat gtatgacgaa gtaccgccag
      961 gcacgaaacc tttcgatgaa gcgaataaat tagtttttgc taccggccca ttaactggat
     1021 ctggtgcccc ctgtagttct cgcgtaaata tcacctcact ttctactttt accaaaggaa
     1081 atttagtcgt cgatgcccat atggggggct tttttgcagc gcaaatgaaa ttcgctggat
     1141 acgacgtcat tattatcgaa gggaaagcga aatcaccggt atggctgaag attaaagatg
     1201 acaaagttag ccttgaaaaa gccgatttct tatggggaaa agggacgcgc gcaacgacgg
     1261 aagaaatttg tcgcttgacc agtccggaaa cctgtgtggc ggctattggt caggctgggg
     1321 aaaaccttgt tcctctctct ggcatgttga atagccgtaa ccacagcggt ggtgcgggaa
     1381 ctggcgcaat aatgggttcg aaaaacctga aagcgattgc ggttgaaggg acgaaagggg
     1441 tcaacattgc cgatcgtcag gagatgaagc gtctcaacga ttacatgatg actgaactta
     1501 tcggtgcgaa taacaaccat gtcgtgccaa gtacgccgca atcgtgggca gagtattcag
     1561 atcccaagtc acgttggact gcacgtaaag ggttgttttg gggcgcggct gaaggtggtc
     1621 cgattgaaac gggtgagatt ccgccaggca atcagaacac ggtcggcttt cgtacctata
     1681 aatccgtttt tgacttaggt ccggcggcag agaaatacac agtaaaaatg agtggctgcc
     1741 actcttgccc gatccgttgt atgacccaaa tgaatattcc tcgggtgaaa gagtttggcg
     1801 tgcccagcac aggtggtaac acttgtgtag caaactttgt ccataccacc atcttcccga
     1861 acggtccgaa agattttgaa gataaagatg atggtcgtgt gattggtaac ctggttggtc
     1921 tgaatctgtt cgatgactac ggcctatggt gtaactatgg tcagcttcat cgcgacttta
     1981 cctattgtta cagcaaaggg gtgttcaagc gtgttctgcc tgctgaagag tatgcggaaa
     2041 ttcgctggga tcaactggaa gcgggtgacg ttaacttcat taaagatttt tactaccgtc
     2101 tggcgcatcg cgtgggtgag ctgagtcacc tggctgatgg ttcatatgcc atcgcagaac
     2161 gctggaattt gggtgaagag tactggggct acgcgaaaaa taaactctgg tcgccgtttg
     2221 gctatccagt tcaccacgcc aatgaagcgt cagcgcaggt cggctccatt gttaactgta
     2281 tgttcaaccg tgactgcatg acgcataccc atatcaactt tattggttcc ggcttgccat
     2341 tgaaactgca acgtgaagtg gcgaaagaac tttttggttc tgaagatgct tacgatgaaa
     2401 ccaaaaacta cacgccaatc aatgacgcaa aaattaaata tgctaagtgg tcgctgttgc
     2461 gggtctgttt gcataacgcc gtcaccctgt gcaactgggt ctggccaatg accgtttcgc
     2521 cgctgaaaag ccgtaattat cggggcgatc tggcgcttga agccaaattc ttcaaagcga
     2581 tcactggcga agatatgact caggaaaaat tagatttagc tgcagagcgt atctttacgt
     2641 tgcatcgcgc ctacacggta aaactgatgc aaactaaaga tatgcgtaat gaacacgatc
     2701 ttatctgttc ctgggtattc gacaaggatc cgcagatccc ggtctttact gaaggtacag
     2761 acaaaatgga ccgtgacgat atgcatgctt cgctcacgat gttctacaaa gaaatgggct
     2821 gggacccaca gcttggttgt ccgacccgca aaacattgca gcgtctgggg ctggaagata
     2881 ttgccgccga tctggcagca cacaatctac tgcctgcgta aggaatgggt gagatgaacc
     2941 atcaggatga attgccgtta gcgaaagtca gcgaagttga tgaggcgaag cggcaatggt
     3001 tgcaggggat gcgtcacccc gttgatacgg taaccgaacc agaacctgct gaaatcctgg
     3061 cagagttcat tcgtcagcat tctgcggctg ggcagctggt ggcgcgcgcc gtgtttctct
     3121 cgccgcccta ttcggttgca gaagaggaac tgtcagtttt gctggaaaac attaagcaga
     3181 acggagacca cgcggatatc gcgtgtatga cgggaagcca ggatgattac tattactcaa
     3241 cgcaggccat gagtgaaaac tacgcggcga tgtctttgca agttgtggag caggacattt
     3301 gtcgcgccat tgcacacgcc gttcgttttg agtgccagac gtatcctcgt ccttacaaag
     3361 tcgccatgct gatgcaagcg ccatattact tccaggaagc gcagatcgaa gccgcgatcg
     3421 ccgcgatgga tattgcacct gaatatgctg atatacggca ggtggagtca tcgacggcgg
     3481 tgttgtactt gtttagcgag cgatttatga cctatggcaa ggcgtatggc ttatgcgaat
     3541 ggttcgaggt agagcagttt cagaacccct gaaatttcag ggagatgtca tggatagggt
     3601 ggacagtagc agccactgca ttaggggata atcagatgtc cttcactcga cgcaaatttg
     3661 ttctggggat gggaacagta atctttttta ctggttctgc gtcttctctg ttagcgaaca
     3721 cgatgcaaga aaagaaagtt cggtatgcca tgattcatga cgagtcacgg tgcaatggct
     3781 gtaatatttg cgcccgcgca tgccgtaaaa ctaaccacgt ccccgctcag ggaagccgtt
     3841 tatcgatagc acacattcca gtcaccgata acgacaacga aacgcagtat cacttttttc
     3901 gccagtcatg ccagcactgt gaagatgctc cttgcatcga cgtttgcccg acaggggcgt
     3961 cgtggcgtga tgagcagggg atcgtgcggg tagaaaaatc gcagtgcatc ggctgcagtt
     4021 attgcattgg cgcttgtcct taccaggttc gctaccttaa ccctgtcact aaagtggcag
     4081 ataaatgtga tttctgcgct gagtcccgac tggcaaaagg ctttccgccc atttgcgtca
     4141 gcgcctgtcc ggaacacgca ttgatctttg gtcgcgaaga tagcccagaa attcaggcgt
     4201 ggttgcagga aaataaatac tatcaatatc agctacctgg tgccgggaaa ccgcacctgt
     4261 atcgccggtt cggtcaacat ttgattaaaa aggaaaatgt atgaacccgt cgcaacatgc
     4321 tgaacagttt cagagccagt tggcgaacta tgtgccactg ttcactcccc aattttggcc
     4381 ggtgtggttg atcattgccg gactattgct ggtagggatg tggttggtgc tggggctgca
     4441 tgccttgctt cgtgctcgtg gcgtgaagaa atcagccaac gaccatggtg agaaggttta
     4501 tctttacagc aaagcggtca gattatggca ctggtcgaat gcgttactct ttgtattgtt
     4561 gctggcaagt gggctgataa atcactttgc gctggtgggc gcaactacgg ttaaaagtct
     4621 ggttgcggtg catgaagttt gcggattttt gttactggca tgctggctcg gctttgttct
     4681 gatcaatgcc gttgggggta atggtcacca ctatcgcatt cgtcgtcagg ggtggctgga
     4741 gcgagcggca aaacaaacgc gattctattt gtttggcatt atgcaggggg aagaacatcc
     4801 tttcccggca acaacccagt ctaaatttaa tcccttacag caggtcgcct atgttggtgt
     4861 catgtatgga ttgctgccat tgttactatt gacggggctg ctgtgtctct atccgcaagc
     4921 cgtgggagat atgtttcctg gcgtaagata ctggttattg caggcgcatt ttgctctggc
     4981 atttataagc ctctttttta tcttcggtca tctttatctt tgcaccacgg ggcgtacgcc
     5041 acacgaaacc tttaaaagca tggtcgatgg ctatcaccgg cactaacgaa tgatcattac
     5101 acgagcagac ctgcgagaat ggcgtatcgg agcggtgatg tatcgctggt ttctgcgcca
     5161 tttcccacgc ggcggtagtt atgccgatat tcatcatgca ctgattgagg aaggatatac
     5221 ggactgggcg gagagtctgg ttgagtatgc gtggaaaaaa tggctggcgg atgaaaactt
     5281 cgcgcatcag gaagtttctt ctatgcaaaa actggccaca gatcccggag agagagcatt
     5341 ttgcagtcag tttgcccgca gcgacgatca tgctcgcatc ggctgttgtg aggataatgc
     5401 cagaatcgca accgctgggt atgccgcgca aattgccagt atgggttata gcgtgcggat
     5461 tggaagtgtc ggctttaaca gtcacattgg cagctccggt gaacgcgcac gggttgctgt
     5521 aaccggtaat tcttcgcgga taagcagcgc tggagatagc agtcggattg cgaataccgg
     5581 aatgcgagta cgcgtttgta cattgggtga acgatgtcat gtcgccagta atggtgacct
     5641 ggtgcaaatc gccagctttg gcgcaaacgc cagaattgcc aacagcggcg acaatgtaca
     5701 catcattgcc agcggtgaag attcaacaat agtgagcacg ggcgtggtcg actcaattat
     5761 tctcgggcca gggggaagcg ccgcactggc atatcatgac ggcgaaagag ttcgcttcgc
     5821 cgtcgccatt gaaggtgaaa acaatattcg agccggagtc cgctatcgcc ttaatgaaca
     5881 gcatcagttt gttgagtgct gatagcttta catttgatta aacgaaagac gccgacgagc
     5941 acgggatgca ggctttacga ccgcctgagc tatcgcctca ccaatttctg cacatgccgt
     6001 aagtccctgt acgaatggct ggtcgtgcat caaccagggc aacgcgccga acgctacgcg
     6061 cccacgaatt tcttcgggtt gctgtagcgt ataatcttcg ccaacatcag ggatttcatc
     6121 ccctgttttc tgtaattgtt cgcgtagtcc agggaaagga atatctttca ctttaagcgg
     6181 acgctgccca cgggcatcaa taaaaacgtc aaacgagtag ctattgtctt ccgttttcag
     6241 gacggtgcgc gactcattaa tttccatttc gtagtcttca ccgagggcga gaatatgaat
     6301 gattcccgct tcacgtaagg ccagtagtcg acgaatagac tctgaaggga ttgccgcata
     6361 attatcgatg aaaacccggg caaggccttt actgaaccgt ttatggtcgt gttcattcag
     6421 atgaggaaca atttcctgta cggcttcatg caggcgcaga atgacataac gccagggaac
     6481 agtatgtttt tctcgtttat tgcgttcgac ttcctggaga tttttttctg cccagtcgaa
     6541 tgggtcgcgt tgtttgcgct cggcaaacca ggcttgagca aaggaatcga cattcaggct
     6601 ttctaaggct atgcgttggc tccagtctgg atcagcaaat ttgatttcct ctactatcaa
     6661 tctaaatacc cgatccaaaa ggcccacttc gcctttttga atctcagcat ttaatgcttg
     6721 atcggtgacg atgtgtaagg gctcgtaggg aatagggcaa tagaaatcgg cttcgggtaa
     6781 aatacccgta cgcgacatca acgtaatatt tagcttttca cttgcgttat cgcggtgaaa
     6841 aacgacgtgt tgtttatcat cttcaatgaa cgaaccatgc tgaatagcca ctgccattgc
     6901 cgcatccagt ccgctcaagg atgttcccat aatacccacg ttacacgcat cgacctttgc
     6961 ttccatcagg cctgaccacg ggctgggaaa atacgttcgg gttgcttctt cttcatcagg
     7021 ccagacgtga cccgtggcga tcaccgctaa atcaaacgtc tcgctgggta aatcctgatt
     7081 tgtagcgagc atgacgccag catttgtaat ttgcagatcg gtaacctggc atgattcata
     7141 aacagccact gcaaattttt gctgtcgtgc ctggtctact aatcgtaaaa attgatcgcg
     7201 gaaatattcg cccagcagaa ttcgcggtaa aaactgacga tcgtgcaagg tttctttttt
     7261 aacgccataa cgctggagat ggctggcttc ttgcttttgt agccattcga gatacgtaca
     7321 ataaatcggc ggtatttcaa tactggcaat atttgccagc atcatttttg agttttcctc
     7381 atcactgtat ggcatcccga caccggcttc atcagcctgc tcgaagatag aaatagaaag
     7441 tggagtttgt tgctgtagaa gcgagaataa ggtgtagatc cccgtaggcc cggcacccac
     7501 aatagcaatt tttttcattg ttacttacct gtacgcattc tttcgaattt agtgttatca
     7561 gataagtttg gttgagattt ttagaatttg agaaatttca gacagggatg aaaagggcgc
     7621 taaccgccct tgattaaatt ctgtcaggcg agttttgcct gattgatttg actaagtggc
     7681 tcattgacat cgaaaacttt ggcgacaact tcctcaacgc cgagattttt cagtcgggca
     7741 gtatgttttt ccagataggc aagggcgctt ttttcattag taaaaaggta gatcccaccg
     7801 gcttcgtggt tcttttcact ttctgtccat accttccaca gaaaaccagg ttcctgatta
     7861 atcgactcag caagcggttt aagctgctca gccattgcgt cgccaaatgg gccattgaaa
     7921 gaaaaatgaa gttgtaacaa agttgccata gcgattctcc ttctgataac gcatacgaag
     7981 acagtgtaaa tcttttaacg tattggatat agagaagaaa cagagggaaa aaggagaaga
     8041 ccagattaag aaaatctggt gcgtccgagt ggactcgaac caccgacccc caccatgtca
     8101 aggtggtgct ctaaccaact gagctacgga cgcaggatgg tgcgttcaat tggactcgaa
     8161 ccaacgaccc ccaccatgtc aaggtggtgc tctaaccaac tgagctatga acgcaacgtt
     8221 gtaggtgaca acggggacga atattagcgg cagagtggga aggtggcaag aggcaaaacg
     8281 taattttctg cgctatttcg accgtttgca gagcttttaa gcaaattggc tatattttgt
     8341 tgatttgcaa gggcgatttt tattcaggat cgcatttaca tcagatacaa cccaataatc
     8401 cgtgtatcag agtttaaaca gagttgtttg ccaccatctc aaaatctgtc gtggataaat
     8461 cctccgtagc ctttacactt aggtagcaat aaaggcacac agatgcagca aaggaataaa
     8521 tcatgggatc tgatgcgaaa aacttgatga gcgacgggaa cgtgcaaatt gttaagaccg
     8581 gcgaggtcat tggcgcgacg caacttactg aaggtgagtt aattgttgaa gctggcggaa
     8641 gagccgaaaa taccgtggtc acgggggctg gctggttgaa agtggcaacc ggtgggatcg
     8701 ccaaatgcac acagtacggc aacaatggca cgctatcggt cagcgacggt gccattgcca
     8761 cagatattgt tcagtccgag ggaggcgcaa ttagtctctc tacgctcgct acggttaatg
     8821 gccgccatcc cgaaggtgaa ttcagcgttg ataaaggtta tgcctgcggt ttgttgctgg
     8881 aaaatggcgg taacctgcgt gtactggaag gccatcgcgc ggaaaaaatt attctcgatc
     8941 aagagggtgg cctgttggtc aatgggacaa cctcagcggt cgtggtagat gaaggtggtg
     9001 aattgttggt gtatccaggt ggggaagcca gcaattgtga gattaatcag ggcggcgttt
     9061 ttatgctggc ggggaaagcc agtgatacat tgcttgctgg tggcaccatg aataatctcg
     9121 gtggtgaaga ctctgacact attgttgaga atggatccac ttatcgtctg gggacggatg
     9181 gtcttcagct ctacagttcc ggtaagacgc aaaacctgtc cgttaatgtg ggtggtcggg
     9241 ctgaagtgca tgccggtacg ctggaaaatg cggtaataca aggtgggaca gtcatcctgt
     9301 tgtcacccac cagcgcggac gaaaattttg tcgtagagga agatcgcgca ccggttgaac
     9361 tgaccggtag tgttgcatta ctggacggcg cttcaatgat tattggctat ggcgcagatc
     9421 tgcaacaatc aacgattact gtacagcagg gcggtgtatt gattctcgac ggcagtacgg
     9481 taaaaggtga cagtgtcact ttcagtgttg gtagcatcaa tctaaatggc ggaaaactgt
     9541 ggctaatcac tggtgcggca acgcatgtgc aactgaaagt gaaacgcctg cgcggagagg
     9601 gagcgatttg cctgcaaacc agtgcgaaag aaatttcacc tgacttcatc aatgtgaaag
     9661 gggaagttac tggtgatata cacgttgaga taacagatgc cagtcggcaa actctgtgta
     9721 acgcactgaa actacagcca gacgaagacg ggattggcgc aacgctccag cctgcgtaaa
     9781 aacacaggcg caacccgtga aggttgcgcc ttgtctttac tagcgagctg ctcgttgcag
     9841 aatgatggct gacggcagac gttgcaggaa ccgcatacgc agcatcatca taatggctgc
     9901 cgacgtcagg ccaataataa agcctatcca gaagcctgct ggccccatag gttcaacgac
     9961 cagatcggtc agtgccagaa tatagccgct tggcaagccc agcacccagt aagccgtaaa
    10021 ggtaatatag aaaatggaac gcgtatcttt atatccacgc aaaatcccac tgccaatcac
    10081 ctggattgag tcagaaatct gatataccgc cgccagcaac atcaaatgcg cagccagcgt
    10141 tacaacctcg ggattgtcgt tgtacaacag ggcgatttgc tcccgcagtg aaaccgtgaa
    10201 aatggccgtc agggttgcca tacagacacc caccataagc ccggtccgcg cagcggtttg
    10261 cgcatccagc gttgagccct gacccagacg ataacctacg cggatggtta ccgctgccgc
    10321 cagcgacatg ggaagtacga acatcagtga actaaagttc agggcaatct gatgtcctgc
    10381 gacatcaaca ataccgagcg gagacactaa cagagccacg acggcaaaca gcgttacttc
    10441 aaagaacagc gccagcgcaa ttggcaaacc gagttgaatc agtcgtttca taaccgcagg
    10501 atcgggcttc gcagtgcctt tttcgttacg aatatcgcgc atcgagcggg cgcgtttaat
    10561 gtaagaaacc atggcaagga acatgaccca atacaccgca gcagtagcca cgccacaacc
    10621 aacgccaccg agctcaggca taccgaaatg accgtaaata aagatatagt tcaccggaat
    10681 gttcaccagc aggccgataa agcccatcac catacccggc ttggtttttg ccagaccttc
    10741 acactggtta cgggcaacct ggaagaaaag atagcccggc gcgccccata gcaacgcacg
    10801 cagataaccc acggctttgt ccgccagagc cggatcgatg ttttccatgg agcggataat
    10861 gtaacctgca ttccacagca ccagcataat gagaacggaa acaaaacccg ccagccagaa
    10921 accttgtcgc acctgatgcg caatgcgctc acgtcgaccg gaaccattta attgcgcgat
    10981 aaccggcgtt aatgccagca gcagtccgtg accaaagagg atcgccggaa gccagataga
    11041 agtaccgata gcgaccgccg ccatgtcggt ggcactatag ccgcccgcca tcacggtatc
    11101 gacaaaaccc atcgcagttt gggcgatttg cgcgagaatc accgggattg ctaatgctaa
    11161 taacagacgc gcttcactga tatacttctg cacgtgaaca ccttttattt gtagttatat
    11221 gagagactaa aaagccgcct gaatgggcag caaaaagaag aagcagggga aattcagtct
    11281 attgtagcga ggatttatta attctccagt gaaaaaatag tcattcttac cccttcactg
    11341 gtaagggcgt ttgccacctg ctattgtgct gaacagaatg tcttaactga tttcaggagt
    11401 tgtaagtatg tttacgggga ttgtacaggg caccgcaaaa ctggtgtcga ttgacgagaa
    11461 accaaatttt cgcacacatg tggtggagtt acccgaccac atgctggacg gtctggaaac
    11521 cggtgcttcc gtggcgcata acggttgctg cctgaccgtg acggaaatta acggcaacca
    11581 tgtcagtttt gacctgatga aagaaacgtt acgcattacc aatcttggcg atttaaaagt
    11641 gggggattgg gtaaacgttg agcgtgcggc gaaattcagt gatgaaattg gcggacactt
    11701 aatgtcaggt catattatga ccaccgctga agtggcgaaa atattaacct cggaaaataa
    11761 tcgccagatc tggtttaaag tccaggatag tcagttgatg aaatatattc tgtacaaagg
    11821 atttattggc atcgacggta ttagcctgac cgtcggtgaa gtcacgccaa cgcgtttttg
    11881 cgtccattta attccggaaa cactggaacg cacgactctt gggaagaaaa aacttggcgc
    11941 acgcgtcaac attgaaatcg atccacaaac tcaggcagtg gtagatacgg tagaacgagt
    12001 gctggcggca cgagaaaatg ccatgaatca accaggtaca gaagcctgat ctgaaatcgc
    12061 ccccggagag ggggcgatag aatagccttt agcgagccac tcgaaggccg ttttcaacac
    12121 cgcgtgagaa cacgacctgc cagagctgaa tatcacgggc gcggaaagca cccgcacagg
    12181 cattcagata ataggtaaac atccgtttaa agcgttcact gtagttatcc gcaatttctg
    12241 gccatgcggc gaggaatcgt tcataccacg ccatcaacgt agtatcgtaa tcagcaccga
    12301 agttatgcca gtcttccatc acaaagtggg gttcgctgga ctgagcaatc tggcgtacag
    12361 agggcaggca accgttcgga aaaatatatt tattaatcca gggatcaaca ttcagatcgg
    12421 tttttttcga accgatagta tggagcagga atatgccttc cggtttcaaa ttacgatcca
    12481 ccaccgcaaa ataggtatcg taatttttcg gtccgacgtg ctcgaacatc cccacagaaa
    12541 caatacgatc aaactggtcg ttcaggtcac gataatcttg cagcaaaatg gtgacatcca
    12601 ggccttcaca gcgttcctga gccatttttt gctgttcggc agaaatggtg acgccgacca
    12661 cgcttacgtc ataattagac gccatgtagt gcgccagtcc accccagcca cagccaatat
    12721 ccagtacgcg catccctggt tttaactgca atttttcaca aatcattttg agcttcgcct
    12781 gctgggctga ttccagatta tcggcatctt tccagtaagc acaggaatat tgcatgaagg
    12841 gatcaagcat gcggctgaac aagtcattac ccaaatcgta atgctctttg ccgactatcc
    12901 aggcacgttt tttactctgc agattgaaga gacgagcgcc ggcaatacgc agcgtgtctt
    12961 tgaaatgatg ggggagttgg ttctcgagac ctgcgcgtaa gactttgcta aaaaacatat
    13021 ccagtcggtc acattcccac cagccatcca tataactttc gcctaacccc aacgatcctt
    13081 cctgcagaac gcgtttaaaa aaatcggggt ttttcacacg aatatccgcc ggggcagaac
    13141 cgttaatggc tataccggca cggctaagta attcgttggc gatacggtac cagttgtcat
    13201 ccggtacact gacttcttct atacacgatg aactcatagt ttctccatca ctggtcggtg
    13261 atcagaacct taaaacagcg tagacgcttt ttgggctttg tgagaaatct cgcagaaaaa
    13321 accgcgagtc tataatccga cgtagaacag agggaaagga ggaacccttg ccgaaatgac
    13381 catccatagt gagtcgggaa cgatcctgtc cccgtaaaaa cataatattt atcgtagctt
    13441 taacaactcg aaattagtat aggccgctaa tttcaatcat acaacaaaat tttgttagca
    13501 gcctaagtat aatcatatcg atatagatca gtgtgattcg ctatgagcga cttcggcatt
    13561 gccatgattc tgacagtcta cttcttcaca acgttgcatc atgtaaccca gagcgaccag
    13621 cactactgtt gataacatca cgctggtggt ggtgagcaat ggcgtgctga tactgatcag
    13681 ccaggaaact accagacttg cgaggaagca cagacccagt tgtagagtgt tctgcaacgc
    13741 tgcggcgcga ccggttgcat gtgggaaggg acgcagcgcc tgggcgacaa caatcgggta
    13801 gatcgcgcca ttggcaatcg ccatcacaca gaatgggatc aggatttcga ctagcgacac
    13861 atggctaatg aagcccgcag cccaggtcgc aatgacgctg acagcaaaca gtaccagcaa
    13921 ccacggtaat aactgcttgc cttgccattt ctgcagtgcg gcgcgacagc cataaccacc
    13981 aatcagaaac gcgatagttt gcgggacata acttaaacca ataactgccg ggctgtagcc
    14041 catttcacta aggatgaacg gtgagccggt cagccatgca aaaaaactgg ctgaacaggc
    14101 cgcatatatc agcacgttgc cgcgataggt tttagaacgt agcaggtcgg taaaggtcag
    14161 accatcctga ctattgttac gggccttcgt cgtgggtttg agccagaaaa taggcagaat
    14221 cagcaccacg gtaatggcaa acagggtggc gaaaatcgcc tgccaggaaa aatggaccag
    14281 cagccagctt cctaacagag gagccagtgc cggagataga cccaccagcg gcatgatggt
    14341 cgcaaaaata cggttaactt tctgtgaagg ataataatcc gtcactaacg cttgccagat
    14401 aactgccgcg gcgcagacac ccacagcctg tacaaaacgc aataccagca gcgtagcggc
    14461 gttttctacc cacagcatcc ccagactacc taacgcaaaa attgtcaggc cgattaataa
    14521 taccggttta cgaccataac ggtcggagag cggcccccac agaagctggg ctgcggcaaa
    14581 accggcaagg aacagactaa ggctggcact gacagcagac gcaggcgttt gcaggtcggc
    14641 ctgtatggcg gcgaaagcag gcagatacat atcggttgcc agaaaaccga gtacgctcaa
    14701 acccgccagc cagactaaaa atcttttccc aggttgcatg tatattctct aaatctctca
    14761 attaaacacc gccgcaaagt gtagggagtg aaattagtgt tgtgaaacgg taatatttga
    14821 tgactggttt caaaaatttt gcaggcagac tatgtggtca gaatattcac tcgaagttgt
    14881 tgatgcggta gcgcgtaatg gtagttttag cgctgcggca caggagttgc atcgcgttcc
    14941 ttctgcggtc agctataccg tgcgtcagct ggaagagtgg ctggcggtgc cgctgtttga
    15001 acgacgtcac cgtgatgtgg aactgaccgc tgccggggcg tggtttctca aagaagggcg
    15061 ctctgttgtc aaaaaaatgc agatcacccg gcagcaatgt cagcagatag cgaacggctg
    15121 gcgcggtcag ttagctatcg cagtggataa tattgtcagg ccagaacgta cacggcagat
    15181 gatcgttgat ttttatcgcc attttgatga tgtcgaactt cttgtctttc aggaagtgtt
    15241 caacggtgtc tgggatgcgc tttccgacgg gcgcgtggaa ctggcgattg gcgcaacacg
    15301 cgcgattccg gtaggcggtc gttatgcctt ccgcgatatg gggatgctaa gctggagttg
    15361 cgttgttgcc agccaccacc cgctggcgtt gatggatggc ccgttcagcg atgatacgtt
    15421 gcgcaactgg ccgtcgttgg tgcgcgaaga cacctcgcga acgctaccta aacgtattac
    15481 ctggttgctg gataaccaaa aaagacttgt cgtgcctgac tgggaatcat cggcaacctg
    15541 tatctcggca ggattatgca tagggatggt gccaacacat ttcgccaaac cgtggcttaa
    15601 tgaggggaag tgggtagcac tagaactgga aaaccccttc ccggattcgg catgttgtct
    15661 gacatggcag caaaatgata tgtcgcctgc gttaacctgg ttgcttgagt atctcggcga
    15721 tagtgagacg ctgaataagg agtggctgcg ggagccggaa gagactcccg caacgggtga
    15781 ttaacgacga tagtcgcgga acgggccgtc agccacggag cggcgttcaa tcaagcgcgg
    15841 atgcacttca atggactgcg gttcttcgcg tttattgacg atacgatcca acagcatgtt
    15901 gaacgccgtt tcgcccagcg aatcttttgg ctgatggatc gtggtcagcg ctggcgtaaa
    15961 atagcgggcg ttgcgcacgt tatcataacc gatcagcgaa acatcctgcg gcacgcgcag
    16021 acccatttca tcagcagcac aaagtgcgcc cattgccatg atatcgccac cacagaagac
    16081 ggcagtaggg cgatgcggtt gcgacagtat ttgctgcatg gcgcgataac cggattcagg
    16141 ttcaaagtca ccctgtacaa tccagctttc cggcactttg atcatcgctt cttccatcgc
    16201 cttcataaaa ccggcaaggc ggcctgcgcc ggtgttacgt tccagcgggc cagggataac
    16261 gccgatttcg cgatgaccgc gttcaatcag ataacgcccg gccatgtagc cgccttcgaa
    16321 tgcgttatca atgaccgcat cggtgaagtc agcttttgct tcaccccagt ccatcaccac
    16381 cattgggata tggcgatact cttccagcat cgccagcaac ggctctgggt actcagaaca
    16441 catcaccagc agaccatcga cgcgtttttg cgccatcatc gacagataag cccgctgttt
    16501 ctcaagattg ttccacgcat tgcccagaat cagggtgtaa cctttctgga agcaattttt
    16561 ttcaaccgct tcaatgatct cggcaaaata ggccgcttcg ctgctggtcg ccagcaaacc
    16621 gatagacttg gtgtggttaa ccttcaggct acgcgccacc gcgctagggg agtagtgtaa
    16681 ttctttaatc gctgcccaca cggcgttgcg cgtttcttca gcgacgaaac gtgttttgtt
    16741 gatcacgtgt gacacagttg tagtggaaac gtttgctcgt ttcgctacat cttttattgt
    16801 tgccattcca tttcactcca gaccctaact tcagctcctg caaaatcgca aggtaaacgt
    16861 ttgcctttac acaccttacg cacaacaaaa gtgttgcggt acgccggaaa aacgacacgg
    16921 agcgtcagga aggggtcaat ggccagtacg caaatagtgt aagcgtggaa ttttggccga
    16981 tcttgacgaa aaggggaagt ggtaaaaaca cttatcatta taaatgacgg gagatttttt
    17041 catcacagtg tgtaaaaatg cgtaatatca ctttttgtgt ggtaataaaa aaggagaaaa
    17101 cttgatgagc accgacctta aattttcact ggtaacaacg attatcgtcc tcggtttgat
    17161 cgtggtcgtg ggtttgactg ccgcgctgca ctgatttcct ctcgagggag cgcgttctcc
    17221 ctcgcatctc cttcctgttt gttagcaaaa tggcaaaagt gttttctctt tttgttattt
    17281 tcattttttt gtgattgctg tcatgctttc gccactttaa tgaatatcgc gtaatgacgc
    17341 gaagaggtgg agataaggca tgaaaattaa ctatccgttg ctggcgctgg cgattggcgc
    17401 gtttggtatc gggacaacgg agttctcgcc aatgggcttg ttgcccgtca ttgcgcgcgg
    17461 tgtggatgtc tcgattcccg ctgccggaat gttaatcagt gcttatgcag ttggcgtaat
    17521 ggttggcgcg ccgctgatga cgcttctact ttctcatcgt gcccgccgca gtgcgttgat
    17581 tttcctgatg gcgattttca cgcttggcaa cgtactttcc gccatcgcgc cggattatat
    17641 gaccctgatg ctttcacgca ttttgaccag cctgaatcac ggggcatttt ttggtttggg
    17701 ttcagtcgtg gccgcaagcg tggtgccaaa acataaacag gccagcgcag ttgccactat
    17761 gtttatgggg ttaaccctgg caaatatcgg tggcgtgccg gcggcgacct ggttgggtga
    17821 aaccatcggc tggcggatgt catttctggc aacggcgggg ctgggagtga tttcaatggt
    17881 aagtctgttc ttctcattac ctaaaggtgg tgcaggggca cgacctgaag tgaaaaaaga
    17941 gctggcggta ttaatgcgtc cgcaggtgct gtctgcattg ctgacgacgg tactgggagc
    18001 tggtgcaatg tttaccctct acacctatat ctctccggta ctgcaaagta ttacccacgc
    18061 aacaccggtg ttcgtcacag caatgctggt gctgattggt gtcggattct ctatcggtaa
    18121 ctatctcggc ggcaaactgg cagatcgttc agttaacggc acgttgaaag gctttttgtt
    18181 gctgctgatg gtgattatgc tggcaatccc gttcctggcc cgcaatgagt tcggcgcagc
    18241 tattagcatg gtggtgtggg gcgctgcaac ctttgcggtc gtaccgccgt tacagatgcg
    18301 cgtgatgcgt gtcgccagtg aagcgccagg tctgtcttca tcagtcaata ttggtgcctt
    18361 taatcttgga aatgcgctgg gagcagctgc tggtggtgcg gtaatttccg ctgggctggg
    18421 atacagcttt gtgccggtga tgggggcgat tgtcgcggga ctggcattat tgctggtgtt
    18481 tatgtcagcc agaaaacaac ctgaaacagt ttgcgttgcc aacagctaat taaacaaaaa
    18541 cgcagaagga taaccttctg cgttacagtt gctggcaacg tgcgatttat cttgccgaat
    18601 gcgctgttaa cgcctcatcc gtgttggcat ggcgcgcgaa gcgcatcagg taatgttgca
    18661 tttgccatca gttattatgc agcgagattt ttcgctacga attcccagtt caccagcgcc
    18721 cagaagtgct ccagatagcc aggacgtgca ttgcgatagt cgatgtaata agcgtgttcc
    18781 cagacatcaa cggtcagcag cggagttgca tcggtggtca gtggagtacc cgcgttagag
    18841 gttgaaacga tagccagttt gccatcgctg tttttcacca gccaggtcca gccagaacca
    18901 aagtttttga tcgctgcatc agtaaactga gctttgaaat cggcaaagct gccaaaagat
    18961 gcggcgatag cttcagcgac ttttccagtc ggttcgccac ccgcgttcgg tgccaggcag
    19021 ttccagtaga aagtatggtt ccagacctga gctgcgttgt tgaatacgcc accttcagag
    19081 ctgcgaataa tctcttccag tgatttacct tcaaacgcgg tacctttaat caggttgttc
    19141 aggttagtga cataagtctg atgatgcttg ccatagtgat actcgatggt ttccgcagaa
    19201 atgtgcggtg ccagagcatc tttagcatat ggtagtgcag gtaattcgaa tgacattgct
    19261 actctccttt attattaatt tgcatacgta caatagcctt attgtgcgta tggatagggt
    19321 agcaaattaa aaggtgagac aaaagatgaa cttaccctgt tgccgtaaca acagggtaaa
    19381 gttttagcga agtgtttttg gggtcattac ccgacgagcg ccaacatagt ggcgctgcca
    19441 gtagtcttca ctgagagaag tgatttgaat ttcttggccc gtgcgcggtg actgaataaa
    19501 tttgccgttg ccgacataca cgccgacatg atcggctgta ccgcgtccct gagtacggaa
    19561 gaagaccagg tcgccgtttt tcagttcact acgttcgata ggtgctgcat cacgcaggtg
    19621 atacatttca ttcgccgtac gcggaatacg aattttcacc aaatctttat aagcgtaata
    19681 aaccaggccg ctgcaatcaa aaccggtacg cggtgagctg ccgccccaac gatatggctt
    19741 accaatttgt tgcatcagtt tattcattgc cactttcgtg gctttttgca ccttcgcttt
    19801 gtgcgcgtcg gcaagggttc ctgacgcggc atttttgact ttcacacaat gcgatttata
    19861 gcccttacgt ttggtacact tttcggtaaa gttggctgag gccgttttat tgacggcatg
    19921 tttgctgcga gttttggcgt ttttagaagc ggtaatggaa gattttttaa cggaagaagc
    19981 ggttttgctt ttggtcgtac ttttactggc ggcttttttc gctgttttct ggctggcttt
    20041 ctgggtcttt ttggtggttg ccgtgctttt ctttttatct gcttttttgg tgatatgagt
    20101 agtagcagaa ctctccctgg cttgctttga agcatgggcc ataggcgtta aaggcagggt
    20161 ggtaaaaagt aaagcacaga gcgtgatcga aatacggttt atccgcgcca ctgagcgtcc
    20221 ctctgttaag gcaggtctat catgcctgcg tctgatttac aaaacttgat gattctaatg
    20281 catagaagaa gagaaagtta atagctttta ttgttaaaaa atacgtttcg ttggcgagcg
    20341 caaaaaaatg cagcagtgaa agctaaaaat ggttataaat acgccagttt gccgtgcgtc
    20401 agggaagaaa cgaatatttg aaatgcaaca aatagagcaa agggataaaa tagcaaaagc
    20461 gtgatgaaaa tgttattttc cgatgccgca ctgacccgct acaatgtcag attactgccg
    20521 taaagaagtt tgaggaagca agacaatgag caccactatc gaaaaaatcc aacgccagat
    20581 tgctgaaaac ccgatcctgc tgtacatgaa aggttcaccg aaactgccga gctgcggttt
    20641 ctctgcccag gcagtccagg cgcttgccgc atgtggcgaa cgttttgcct atgttgatat
    20701 cctgcagaat ccggacattc gtgcggaact gccgaaatat gctaactggc cgaccttccc
    20761 gcaactgtgg gttgacggcg agctggtcgg cggttgtgat atcgtgatcg aaatgtatca
    20821 gcgtggcgaa ctgcagcagc tgatcaaaga aactgccgct aaatacaagt ctgaagagcc
    20881 ggacgctgaa taatccgtcc tgcaatactg acggatgcga acgggcattc gtcagtcctt
    20941 tctctatccc caatccagcc cttgtggcga acttgaaaat cccgcctcac gcagcagcgt
    21001 aaacatcggc gtttgccgga ccggtaggtc attcacttct gttagcgtaa agcgcagccg
    21061 tggttcgcga cgcagtgcgg tagtcagcgc gtggaaaatc tctggggcga gtaattcctc
    21121 tttttcctgc cagaccagca tttttttgcc accttgcgcc agatagagta acaatttgcc
    21181 gccagaaacg accaccagcg cacccgcccg acgcgttggt atcagcgttg ccggatgtgc
    21241 aggccagggc agaagatttc cccatgcatt agccggatcg ttggcagaga gcgccactgg
    21301 tgtatagtgg cgcgtttgcg tggcttgtgt cgccagatcg cgcaatcggt caatagtcag
    21361 acgttcagcg aattgcgcgc cacccagacc ttctacaaaa cgacctcgca taattcgccc
    21421 ggagtcttcc atacttcgac aaagcgtttg catcgatgga aacccgccag ggatattttc
    21481 ggctatcacc gcctgacgac tgatgatgcc gtagcggtcg agcatatttt ccgccagcgc
    21541 cagcatcctt tcggtatcgt ttagtggctc cacctgcaat aacgaccagc gtccagcaag
    21601 ctttggtgtg ttgtaagata ccagcggcga gacgggttgc gcatagacag gacgtccacg
    21661 acgagcccgg tgactgcggc gagttgaggt gcgtgtgttg gtactgctgc gggtgagggc
    21721 gcgtaacggt gcccaaatgt cgctggtgat gacgccttgc cagaccagcg cccataacgc
    21781 ctcttgcatg gctgagggat caaccgattc gccgatttta tcgcgtatcc gttggctgat
    21841 ttgttgtgca aaccaggctc ctccgtcagc cagaaccgtg actatcgctt gctgcagcgc
    21901 cgaactattc gcctgattcg cttcggcggg agtgaacgat tctgcggcat attcctgtag
    21961 atgcagtgcc accagaccgt catcttcacc cagctttttt tgccccgacc agataaccgc
    22021 accggttgcc agtaattcat cgagcatttc tggcgaatag tcgcgtacgc gggcaggcag
    22081 gatctggctt tcccagagtg aggcgggtaa accgactccg gcaagctgtt cgatcacccg
    22141 catgacgcca tccacgccct cataaacgcc tggcgatgtt gaggcaaaga gtgccgggct
    22201 accatcggtg gcgggtaata cgccctgacg ttccagcagc aatcgcgcat aggtcgtggc
    22261 cgcaacggga cgcgtcgctt ctctggcggc ttgcagcgag cgcaaacgca gacgacgaaa
    22321 tacttcatcg ctcacccaga tgtcttgttg cagattcatc accagcccct gttcgcgcag
    22381 ttgctgaagt tgctcttcga caatggcaat accgagacta aactcatgag ccagttgttc
    22441 agacgtgacc agagcatgag cccggagata gcgcagaaag aggtcgcgaa gcgggtaact
    22501 gactcggtgc aaataaatct ctggcagtga ctctggtagt cgtacgccga gagcatcgcg
    22561 cagcctggcg gcatcatcca tacaggcaag acgctcctgc ccgctaatca tggtcgggaa
    22621 gattcgtttt actgcaagaa gattttccag atacgacgca atctcttcac tgctgcctgt
    22681 atgtcgttgc gccaggtctt caacggtcat tggccccagt tcgcgcagca ggtcgaacaa
    22741 tccttcttca cctttcgctc ttctgccagg agccagtcgt tgcaactctt cttccacctg
    22801 gcgaatgacc tgtgggtcga gtaattcccc cggatcgacc tgtccgagta gattgcgcag
    22861 taactcgctg tccagcgaca gtacggatgc ccggcgctct gccagcgggg cgtcgctctg
    22921 gtacataaat tccgcgacat agccgaacaa taaactggcg gcgaaaggcg aaggcgtagt
    22981 ggtcgttaca tcggatattt gaatttcgcc accgttcagg cgacgcatca aacgttccag
    23041 cgcgggaaga tcataaacat cttgcagaca ttcgcgtagg gtttcgagaa tgaccggaaa
    23101 atctggatat ccctgagcga tttccagcaa ctgactggcg cgcagccgtt gttgccagag
    23161 cggggtgcga tggcccggcg tgcggcccgg cattaataat gtccgcgcgg cgcattcgcg
    23221 aaaacgggcg gcgaaaagtg ccgagctgcc taccgcctcg cggacaattt gcagcaactt
    23281 ttctggttca aacaaaaaaa tcgcggcatc aggcaattta ccatcggtgt caggaatgcg
    23341 cgcaacaatg ccgtcatcac tggcaaccac agacgcgtca gcgccccata gtgcatgtat
    23401 tcgcccggca atcgccagcg cccagggttc atgcacccgt cttccatagg gagagtgcaa
    23461 aataatacgc cagtcaccaa tttcatcacg gcaccgttcg aggaccagat gacgactccc
    23521 cggaacgatg cccgtcgcgt tgcgctgctc gtcaatcaac ccctgaatat tggcgatcgt
    23581 attttcttct gccaaccacg ggggaatagt cccggaaaag aacgcgccat ccgccagtaa
    23641 atggagaaaa tcgccgatca tctcgcctaa ttctgccgga cgtccgttac cttcaccacg
    23701 ccagaaggga aggcgggcag aacgacctgg agcaggagtc acaatcacct gatcgcgggt
    23761 gatttgctgg atccgccatg aggtagcgcc gagagtgata atgtcgttca cccgcgactc
    23821 atataccatc tcctcatcca gttcacccac ccgccgcgaa ccggcctgtt cttcaccttc
    23881 gggtaataac acgctataca tgccacgatc cggaatggtg ccaccgctgg taaccgccaa
    23941 cagctgagcg ccaggtcggg cggtcaatat cccggtctcc ctgttccaga ccagttttgg
    24001 tcgaaaagca gaaaaatcgc cagagggata gcgcccggaa agcatatcca gcgtgacgtc
    24061 aaaaacacgt cttggcagat ctttccacgg tgcggcacgg cgtacgcggg agtaccattc
    24121 gtctgcctgt aatgcatcca tcgccgcggc ggcaacggtt tgctgcgcaa ggacgtcgag
    24181 tggattatgc ggcggtgtca ggttttccag cctgcctgcg aacatacact ctacaatgac
    24241 tgcggaatcg actaagtcac gcctggttcg ggggaaaaaa agccctttag atacaccgcc
    24301 aacctgatgt ccggcgcgac caatgcgttg taacccactg gcaacagaaa gcggcgttgc
    24361 cacctgaatc accagatcca ccgcgcccat atcaatcccc agttcgagac tggaggttgc
    24421 gaccacacag cgtaattcac ccgatttcaa cgcctgttcg gtgattgctc gttgttcttt
    24481 agagacggag ccgtggtgtg agcgggcaat aaaaatgtcg ctactttgta cacggttaga
    24541 ggttgcgccg gaggtcgact cgaaatgggc cgcatcaacg gcgatagatg gggaacgctg
    24601 taagcgtgcg gcgtaaagct catttaatcg tgccgtcagt ttttccgcca gcccgcgtga
    24661 attagtaaag acaatggtcg agcgatggcg caaaacttca tcaaggatac ccgtttcaat
    24721 atatggccag atggagcctt cccggccggc atggctgtct tcgccggtgc cgctggcgac
    24781 cgatgagaca tcatccatat tggcgaccgg tacgacaatc cgtatctgcg gatggcgcat
    24841 tgcgggcggg ttgactaccg taaccgggcg atcgccacca agaaatgctg ccacatcgct
    24901 ggctgaacgc acagtggcag aaaggccaat tcgctgtgct gaggtgtgga gcagcgtatc
    24961 gagccgctcc atacttaacg ccagatgcgc accacgttta ctgcccgcta ccgcgtggac
    25021 ttcatcaata attaccgttt cgacgccgcg tagcgtttcg cgcgcgcggg aggtcagcat
    25081 cagatagagt gattcgggtg tggtgatcag aatatccggc ggattacggg tgagtttgct
    25141 gcgttcctgt gcaggcgtat cgccagtacg gatccccacg cgaagattga cttccgtttc
    25201 gccgcgccgc cgccgttcat cggcaatacc ctgtaacggg atctgcaagt tgcgctgaac
    25261 gtcggtgccc agagctttga tcggtgaaat atagaggatg cgtgaggttt tacgcttatg
    25321 cgcctcgcgg gtatcttcgc cgccttcgcg gaagagctga tcgagggcgt agagaaatgc
    25381 tgccagcgtt ttcccggagc cagtcggtgc aatcaccagc gcatgttcgc ctcgcgccgc
    25441 cacatgccag gtttgcgact ggacagcggt cggctgttta aaggcgcgaa gaaaccagtc
    25501 gcgggtcgcc ggtgaaaaca cgtccggcag gggcgatgaa gggtttagat tatctgccat
    25561 taacgctatt ccccgtgaat atcatgcatg atgcgtggaa tagcctcact atagatgcct
    25621 gaatcattgc atgtcatcag gcatcgactc gattacacct cttcggcggc aggtagcggc
    25681 cagcctccca gacgtttcca gcggttgacg atttcacaaa acagcacagc agtgcgttcg
    25741 gtgtcgtaca gcgcggagtg cgcctgggtg ctgtcgaagt ccatgccagc ggtctggcaa
    25801 gcctttgaca atacggtttg tccgagtgcc agcccggcca gtgcagcagt gtcaaaagtg
    25861 gcgaaagggt ggaacgggtt acgtttcagt gaggcgcgtt ctgcggcggc catcataaag
    25921 ctgtgatcga aattggcgtt gtgcgccacc ataatggcgc ggttacaacc gctcgcttta
    25981 atacctttac gcacaacttt aaaaatttcg tgcagcgcct cgtattcgct gaccgcgccg
    26041 cgatcgggat cgttcgggtc aatgccgttg aaggcgaggg cctctggctg caaatttgcg
    26101 ccgacaaatg gttcgacgtg gaaatgtaat gtggtgtccg gcatcagcca gccttgttca
    26161 tccattttca gggtgatggc ggcaatctca agcagcgcat cggttttggc gttaaatcca
    26221 gctgtttcaa catcgatcac aacaggataa aaaccacgaa aacggtcgca cagaccggta
    26281 agttgagcgt tatcggacat ctgattctct taatacggat aaattgcagc gcgcattatg
    26341 acaaatatta cttgtcgatg cagtaaagat gcgggcgcga tgagttcacg cccggcagga
    26401 gattagttgc ccagaccgcg accggcgtct ttctcttcga ttaactcaat tttgtaaccg
    26461 tccggatctt ccacaaacgc gataaccgta gtgccgcctt ttaccggacc cgcttcacgg
    26521 gtgacgttac cgccgttctg acggattttt tcgcacgctt cagcggcgtt atctacgcta
    26581 agcgcgatgt gaccataagc agtgccgagt tcgtatttat ccacgcccca gttgtaggtc
    26641 agttcaatca ccgcttcttc ggtttccggg ccgtagccaa caaacgccag tgagtatttg
    26701 tattccgggt tttcgctggt acgcagcagt ttcatgccca gtactttggt ataaaaatcg
    26761 atggagcgtt gcagatcgcc aacgcgcagc atggtatgaa gaagacgcat ctttatatcc
    26821 tcaaaatggc taacagattc aaaatgttgt tttagtatag cggcggcttt gcgccgctcg
    26881 caatgttgga ttacagcgtc gggtaatcag tgtagccttc cgcgccgcca ccgtaaaaac
    26941 tttcggcacg ctgtgggtta agctcagctt tgcgttgcaa gcgggcgacc agatccgggt
    27001 tcgcaatcca gtcacgacca aatgccaccg catcaattaa ccctttgccg atcagcgttt
    27061 cggctttttc taccgtgtat gcacctgcgc cgataatcgg accgtggaaa cgggcgcgta
    27121 ctttttcgcg gaacgcatca gtatacggtt caccccccgc ccaatctggt tctgacatat
    27181 gcagataagc aatgccgcgt ttacccagtt gttctatcag atacagtgca tcggcttctt
    27241 cattcgggcc gttatctgtg ttctggaaag taccgattgg cgaaacgcga atgccaatgc
    27301 gatcggcacc ccattcttca atcccggcat cgaccacttc cagcaccaga cgtgcgcgat
    27361 tttccacgct gccgccgtac tgatcggtac gatggtttga agaaggagaa aggaactgat
    27421 gcagcaaata accatgagca gagtggagct ctaccagatc aaaaccggct tcacgcgcgt
    27481 tagcaatggc ctgacggaaa tcattgacga tacccggaat ctcttccagt tcaagcgcac
    27541 gcggcatgga tgtttcaaca cggatcgcct gaccattttc atcgcgcaga gaagtacgtg
    27601 ttcccgcgct aagtgcggaa ggcgctaccg gtgcctgacc gccaggttgc aggctggcgt
    27661 gagaaatgcg tccggtgtgc cacagctgta cggccatatg accattttca gcatgaacgc
    27721 cagcggtaat ttttttccat gcggcaattt gttccggact atggatgcca ggtgcaccag
    27781 catacccttt tgcctgggca gaaatttgcg tggcttcgct aataatcaaa ccggcactgg
    27841 cacgttggcg atagtattcc gccatcaacg gggtagggat gtcacccggt tcaatactgc
    27901 gcagacgcgt cagcggtgcc ataaaaatac ggtttgccgc cgtgatcgcg cccactttca
    27961 gtggggaata cagtttttca gatgacataa aggctcccgg tcgtctggtg ataaaaaatg
    28021 cctgctaaac ggcaggcgtc gcaataatgg tttttacatg tgccagcgcg ttttccagtg
    28081 gctcgaaact gcgcgaaatt ttggcctgca gattcgcacc cagccatagt gcgtaaagca
    28141 cttgtgcctg ttgcagcggt tcgccacaaa aggttaaaca atggctatca cggccatttt
    28201 ccagcgcctg cgagagcagg gcgatcacgc cgcgagcgcc tttatccatc gcgctacgca
    28261 tatcttctga cagatcgcac acttcggcag agagttttac tgtcaggcaa ccactgatgg
    28321 ttccatgttg gcaaaactgg ttcagtgttt gctggtaata agccagtatg cggtcgcggt
    28381 agttaccttc gccggattgc agcaactcag tcagtcgttg gtgatatgtg gcgtaatggc
    28441 gctcaagcat ggcaacgcca aacgcttctt tagagcgaaa gtagtgatag aaggaccctt
    28501 tcggcacttc agcggttttt agtaattcgc ttagccccat cccggtgaat ccacgttgca
    28561 ggcaaagttg ctcgcccgtc gccaggagat gttcgcgagt atcatgttcg gtgtgtttgt
    28621 tcatgcgttg gagtgtagta gaccagtcgg tctagtgcaa gcaaagttgc tactttgggc
    28681 attaacagct tcaataatgg taataatctt atttgaggag ggcgaagtgg cggagcaatt
    28741 agagttcttt cccgttcaga gcccgtgccg gggaatttgc cagtctgacg aacgcgggtt
    28801 ttgtcgcggc tgttttcgta gtcgggatga gcgttttaac tggaataaaa tgagcgatgg
    28861 cgaaaaacaa gaggtgctgc gcctttgtcg gcagcgatta atgcgcaaat tacgcgcaaa
    28921 taagccagcg tcatcagacg aaccggagca accatcactc ttttgagcct aatcttgcgt
    28981 atactcctct catatctgat aagaggaagc gattatggtt cagcgtatta ctattgcgcc
    29041 gcaaggcccg gagttttccc gttttgtgat gggctactgg cgattgatgg actggaatat
    29101 gtccgcccgc cagctggtca gttttattga agagcatctg gatctcggcg tgaccaccgt
    29161 ggaccatgct gatatttatg gtggctatca gtgcgaagcg gcgtttggcg aggcgctgaa
    29221 actggctcct caccttcgtg aacggatgga aatcgtcagt aaatgcggta tcgcgacgac
    29281 cgcgcgtgaa gaaaacgtca ttggtcatta cattactgac cgcgatcaca tcattaagag
    29341 cgccgaacag tcgctaatta atcttgcgac cgattatctg gatctgctgt taatccaccg
    29401 accagacccg ttaatggatg ccgatgaagt ggcggacgcg tttaaacatc tgcatcagag
    29461 cggcaaagtg cgccattttg gcgtatcgaa ctttacgcct gcgcaatttg ccctgttgca
    29521 atcacgtctg ccgtttaccc ttgccactaa tcaggtggaa atatccccgg tgcatcagcc
    29581 gttactgctg gatggcacgc tcgaccaact gcaacaactg cgtgttcgtc cgatggcgtg
    29641 gtcctgcctt ggtggtggtc gtctgtttaa tgatgattat ttccagccgc tgcgtgatga
    29701 actggctgtg gtggcggagg agttaaacgc gggctcgatt gaacaggtgg tttacgcctg
    29761 ggtattacgt ttaccgtcgc agccgctgcc aattatcggc tcaggtaaaa ttgagcgtgt
    29821 acgggcagct gtcgaagctg aaacactgaa aatgacccgt caacaatggt tccgtatccg
    29881 taaagcggca ttgggttacg acgtaccgta agcactttta ggaatagccg ccgttcaaaa
    29941 atgtgtcact ggtttacact tattcaggaa tgcacaatga acggaggtcc tatgaaacgt
    30001 tttagtctgg ccattctggc gctggttgtt gcgaccggcg cacaagctgc cagtgaaaaa
    30061 gtcgagatga acctcgtcac gtcgcaaggg gttgggcagt caattggtag cgtcaccatt
    30121 actgaaaccg ataaaggtct ggagttttcg cccgatctga aagcattacc ccccggtgaa
    30181 catggcttcc atattcatgc caaaggaagc tgccagccag ccaccaaaga tggcaaagcc
    30241 agcgccgcgg aatccgcagg cgggcatctt gatccacaaa ataccggtaa acatgaaggg
    30301 ccagaaggtg ccgggcattt aggcgatctg cctgcactgg tcgtcaataa tgacggcaaa
    30361 gctaccgatg ccgtcatcgc acctcgtctg aaatcactgg atgaagtcaa agacaaagca
    30421 ctgatggtcc acgttggcgg cgataatatg tccgatcaac ctaaaccgct gggcggtggc
    30481 ggtgaacgct atgcctgtgg tgtaattaag taattaagag gccagcgtac cttgtggcgg
    30541 tgcttgctca agttgcgaaa gcgagcagta cagccgccag acaattgccg ccagctcgcg
    30601 ggcggcaggt tgatgatggc gggcaaggct gtcgcaaatc cgctgtaatt cttcaagtgt
    30661 ggccgccagt gatttttgct gaacgccacg ctcactcatc actccccgca acagtgaaat
    30721 acagttatcc cgtactcgcg ataacggatc ggaacgcgat tcccagtcgc gcaattgcca
    30781 gacaacatga gaacagttca gcagcactac accccagcgt aataaccaac ggcgagccag
    30841 cgcatcctgg ctgttactca actgactgac gtgatgataa gtgagcgatt caaattcgct
    30901 ttcactcagt gttggatggc ggcttagttg atcgacaaaa tcccggcgca gcgcgcgtat
    30961 atggcggcgg cttttgcgag catccgatcc tggacgcaga atggcgaacg ctaaccacgc
    31021 caacgcgacg ccaacgattt ttgccagatt atcgttaaga aaatcagcaa aatcatacac
    31081 cgggggatta gtgacagcga taaacgaacc cataaaaaca atcagttgcc cccacagtgc
    31141 ggcaaatttg ggcatctgca atttaagaag ctgcattggc gccagcagtg gaaagagaaa
    31201 cagtaaaaat tgccacagat cgctaatctg caccatcagg ccaaatttga ccacaaagct
    31261 gaatagcgaa agtaacacca gcgtgcgcat cagcagtgac aacgacttaa acggtgctgc
    31321 gacggcggag tagagtacgc agctaattgc tgccagcgtt aatgcattgg aaccggcatc
    31381 ccattgcgaa gcaatactcc atgcgccaat catcatcaac gtacaaaatg tacgcagtcc
    31441 gctccacata gcttcggcgt tatcggtatg acgagccagg ccggatgtcc gtgggagtct
    31501 ggtatgatca tccacaccgc tttgcagatg ttgtaattcc tgactacttt gcagataaag
    31561 gcggcaaaaa taacgtagtc gctgccagaa ggcgacgtgc cgatagtcgg cgacgttggt
    31621 cgggcgtagc ggggcgataa tacgtgcgac ggtgtaaaca tctgtttgcg aactggcgag
    31681 cgccgtcagc agctgttcga gaatttctcg tgtggcacat ggcggtgagg gccagttgag
    31741 caacatacgt cgcaggctgg agatgacact ggtcatacgt aattgctggt ggagcagcgc
    31801 attaaggcgt gcgttttgct ggcgaaaacg atagtggctc cagaaagcct ggatacggag
    31861 caaattcatg gtcagtatct gtccaatcac cccctcatgt gctgcacgaa tggcatcggt
    31921 tgtttcaggc tgccagagta aactggcatg ttccagtaat cgggcgtgca tgtttttcaa
    31981 tgcggttaaa agggctgtag catcggaact gctcggcagg atcatcatca tcatgccgcc
    32041 gcacaaaata ccgacaatca cctcgcaaac gcgcgcctga gcgatatccc acagctggct
    32101 ggcctcagta atattaacca tcggaaaggc gatgatggca gctgtgtagc ccgccagttg
    32161 aaatgcatac gcgacattat tcgtgaagtg cgcacaggcc caggtacaaa agccaagcca
    32221 cgccgccatg ctcaaaagaa aaaaccacgg ctcattgagc gtatgtccgg caagaagcag
    32281 tgccgcaatg gctccgagca aactgccagc gatgcgtccg aggcttttgc tgatgacacc
    32341 gccaacggtg ggaaagctaa ccacggcagc cgaggtcatc gcccaatagg gttcatccag
    32401 atttaaataa taggcaaccg tcagcgccag acacatggca atggtattgc gtaacgcata
    32461 acgccattgc gccagcgtgg ccctgaacca gggcaaattg cgcaaggacc atgacgatgc
    32521 gttcattatt gacctacggc aatactacag gtggtgcccg acaccagcgt gacatcgcct
    32581 gggactttat ccagcgcaaa gcggacggga actcgttggg cgaggcgaac ccaggggacg
    32641 ttaggtttca catccggaat aaggtttgag tcactttcaa cgctctgatc ataaatcgcc
    32701 cgaccgatac tggaaacgtg accctgtaac gttttgttat cactatagag ggtaatttgt
    32761 gctggtgcac cttcgcggat atggcgcaac ttggtttctt caaaataacc aatgacgtaa
    32821 aacgagtggc tgtcgacaag ggcaaacagc ggtttcccgg tgtcagcgta atcgccgatg
    32881 cgggtagtga ggttagtcac ccatccactc accggggcgc gaatttccgt ttgcgccagt
    32941 tgccattgtg cctgtttcag cgtcgcctgt gcggcatcaa cactggcctg catcgcttta
    33001 acattgaggt tagcggtatc gagttcttcg gcagaaataa aattttgtga gagatgacga
    33061 cgacgattcg cttcgttatt ggcttttgcg aggtcagatt gcgctttcgc caactgagcc
    33121 tgtgcgttca gctcggcgat ctgaaagggc gttttatcga tcgtgagcag aagatccccc
    33181 gcgttaacca gttggttgtc ttttatattc agctcaacaa tacgtcccga cacctgaggg
    33241 gtgatagaaa cctgttctgc gcgtattttc ccgtcgcgtg tccacggaga ttgcatgtaa
    33301 taattccaca gccaccatcc ggcaagaacc gcaactaccg ctacaatgat tgttgagaaa
    33361 tacttaattg ttttaattga catagttacc acgcaatcag tattgccagg gaaaggcaaa
    33421 cgcaaatcgc aaacagcgat aaatccatta acaagggatg ccagatgtca ccggcgtaga
    33481 tccagccacg aagcaggcgg tgtacgacaa gccagatgac gaatccaaac gcgaaggctt
    33541 tgaaaaacgg aggaaagtag acggacgcac cgaacaccag atcttgtaag ggcaatcctg
    33601 ttgcattgag cataaacttc acaaagtaga ttcctttacg accgaatata ttgcgttaga
    33661 ttaataaata ttctttaagt gcgaaaaatt tacgcgcaat ttctgaaaaa tatgtctgac
    33721 ggtaaccaaa tgcagcaata catttgtttt agcaatacaa ttgctgcaca ctattctaaa
    33781 agccgcataa tatcttagca agctaattat aaggagatga aattggaatc gccactaggt
    33841 tctgatctgg cacggttggt gcgcatatgg cgtgctctga tagaccatcg cctgaaaccg
    33901 ctggagttaa cacaaaccca ttgggttacg ttacacaata tccatcagtt acctccagac
    33961 cagtcgcaaa ttcaactggc aaaagcgatt ggcatcgagc agccatcact ggtccgtact
    34021 ctggaccaac tggaagaaaa agggttaatt tcgcgtcaaa cttgtgccag cgatcgtcgg
    34081 gctaaacgta ttaaactgac ggaaaaggca gagccgctga tcagcgaaat ggaagctgtt
    34141 attaacaaaa cccgcgcgga aattttacat ggcatctccg cagaggaact ggaacaactg
    34201 attaagctca tcgcaaaact tgagcataat atcattgagt tacaggccaa agggtgaaat
    34261 gaagggggcg tgtggtcagt aacctgacca cacgcaaact tatttagcgc ggggaaacgg
    34321 tcacctgact gccattgctg gccagtacga cacgttggcc cggagagaaa cgagtgttgc
    34381 cttgtttctg taccaccatg atggtattac catcgtcttt acgaatttcc agctcgacac
    34441 cctgcgtttt gttcattgca ctctgtacgc cctgaccggc tacgccacct gcaacagcgc
    34501 ctgcagcagt agccagagaa cgcccggttc cgccaccaac agtattcccc aggaaaccac
    34561 caagaacagc accgccaatt gcgccgataa cgttggaatc atcaccgccc tgaatctgta
    34621 ccggacgtac gttaacgatg gtgccatagc tgacattctg tacttgtttc gcttcagaag
    34681 cggtataaac atcccctgac agggtgtcgt tattaacaca accgacaaga gacagaccta
    34741 ccattgaaac aaccaatacg cgtttaatca ttgaaaaatc tcctgttcac catgaaacgc
    34801 cacgcaagca tccctcatgg tcaaagtata tggcatatgt gaatccatgg ttacatgttc
    34861 taccaaatat gagcaaatca taatgaaagt aggatcaacc aattataaac aaaacattca
    34921 tgatgattat tgatttgaag ataatgtttt actaccaacg gtaaaaatcg gtcattccgc
    34981 atggtattca cgaaaagttt atgtttgagt ggtgcgcaaa cgtcagattg aaaaaggatg
    35041 aactatgaaa tcgggccgct ttattggcgt tatgtcaggc accagccttg atggtgttga
    35101 tgttgtgttg gcgacaattg atgaacaccg ggtcgcacag ctggcaagtt tgagctggcc
    35161 gatcccggtg tctctgaaac aggctgtact ggatatttgc cagggccagc agcttatact
    35221 ttctcagttt ggacagcttg atactcaact cgggcgactt tttgctgatg cggtcaatgc
    35281 cttgcttaag gaacaaaacc tgcaggcgag agatatcgtt gcgatcggct gtcacggtca
    35341 aaccgtctgg catgaaccga cgggcgtggc accacacact ttacagattg gcgataacaa
    35401 tcaaattgtg gcacgcaccg gaattacggt tgtcggtgat tttcgccgtc gcgatattgc
    35461 cttgggagga caaggcgcac cgctggtacc tgcgttccat catgcgctgc tggctcaccc
    35521 aaccgagcga cgaatggtgc tcaatattgg cggcatcgcc aatctgtcac tgctcattcc
    35581 tgggcagccg gttgggggct acgataccgg tcctggtaac atgctgatgg atgcctggat
    35641 ctggcgtcag gccggtaaac cttacgataa agatgccgag tgggcacggg cgggtaaagt
    35701 tattctccca ctgctgcaaa atatgctcag cgacccgtat ttctcgcaac ccgcaccgaa
    35761 aagcaccgga cgcgaatact ttaactacgg ctggctggag cgccatttgc gccattttcc
    35821 gggtgttgat ccccgtgatg tgcaggccac actggcagaa ctcaccgccg tgaccatttc
    35881 tgaacaagtt ttgttgagcg gtggctgtga acgattgatg gtatgtggtg gaggaagtcg
    35941 taatccgcta ctcatggcgc gtctggcggc attactgcca ggcacagaag tcaccaccac
    36001 tgatgccgtt ggcattagtg gcgatgacat ggaagcattg gctttcgcct ggcttgcctg
    36061 gcggacgctt gcgggattac caggaaatct gccttccgtc actggcgcaa gccaggagac
    36121 ggtactcggg gctattttcc ccgctaaccc gtgatatcag agttaactga atttttctta
    36181 ccgcgtctgc cgttacactt ggaacgttag ggagggcgta ttgccctcca gaccaggaaa
    36241 gtcttcggga tatgtctatg aaaaaactgt tactgatctg tttgccggtc cttctcaccg
    36301 gctgtagcgc gttcaatcaa ctgtttgagc gcatgcaaac cgatacgctg gaataccagt
    36361 gtgatgaaaa accgttgacg gtcaaactga ataatccgcg ccaggaagtc agttttgttt
    36421 acgataatca actactgcat ctcaaacagg gcatttctgc gtctggtgca cgttacaccg
    36481 acgggatcta tgttttctgg tcgaaaggcg aggaagcgac tgtctataaa cgcgaccgca
    36541 tcgtcttgaa taactgtcag ttacaaaatc cacagcgttg agatttttcc aggggcggcg
    36601 cacaatagcg tcacccactg acaatccgta aagaaaacca tgtctgataa cgacgaattg
    36661 cagcaaatcg cgcatctgcg ccgtgaatac accaaaggcg gattacgccg ccgcgatctt
    36721 cccgccgatc cattaaccct ttttgaacgc tggctctctc aggcgtgtga agccaaactg
    36781 gcggacccta ccgcgatggt ggtcgctacc gtggatgaac atggtcagcc ttatcagcgc
    36841 atcgttttac tcaaacatta cgacgaaaaa ggcatggtgt tttacaccaa cctcggcagc
    36901 cgtaaagcac atcaaatcga aaataatccg cgcgttagcc tgctgttccc gtggcatacc
    36961 cttgagcgcc aggtgatggt gatcggtaaa gcggaacgac tttcgactct cgaagtgatg
    37021 aaatattttc atagtcgccc acgtgatagc cagattggtg catgggtttc gaagcagtcc
    37081 agtcgcattt ctgcccgcgg tatccttgaa agtaaattcc tggagctgaa gcagaagttt
    37141 caacagggcg aagtgccatt gccgagtttt tggggcggtt ttcgcgtcag ccttgaacag
    37201 attgagttct ggcagggcgg tgagcatcgc ctgcatgacc gctttttgta ccagcgtgaa
    37261 aatgatgcgt ggaagattga tcgtcttgca ccctgaaaag atgcaaaaat cttgctttaa
    37321 tcgctggtac tcctgattct ggcactttat tctatgtcta tttcgcatct ggcgaaaagt
    37381 cgtgtaccgg caaaggtgca gtcgttatat acatggagat tttgatggca agcagtaact
    37441 tgattaaaca attgcaagag cgggggctgg tagcccaggt gacggacgag gaagcgttag
    37501 cagagcgact ggcgcaaggc ccgatcgcgc tctattgcgg cttcgatcct accgctgaca
    37561 gcttgcattt ggggcatctt gttccattgt tatgcctgaa acgcttccag caggcgggcc
    37621 acaagccggt tgcgctggta ggcggcgcga cgggtctgat tggcgacccg agcttcaaag
    37681 ctgctgagcg taagctgaac accgaagaaa ctgttcagga atgggtggac aaaatccgca
    37741 agcaggttgc cccgttcctc gatttcgact gtggagaaaa ctctgctatc gcggcgaaca
    37801 actatgactg gttcggcaat atgaatgtgc tgaccttcct gcgcgatatt ggcaaacact
    37861 tctccgttaa ccagatgatc aacaaagaag cggttaagca gcgtctcaac cgtgaagatc
    37921 aggggatttc gttcactgag ttttcctaca acctgttgca gggttatgac ttcgcctgtc
    37981 tgaacaaaca gtacggtgtg gtgctgcaaa ttggtggttc tgaccagtgg ggtaacatca
    38041 cttctggtat cgacctgacc cgtcgtctgc atcagaatca ggtgtttggc ctgaccgttc
    38101 cgctgatcac caaagcagac ggcaccaaat ttggtaaaac tgaaggcggc gcagtctggt
    38161 tggatccgaa gaaaaccagc ccgtacaaat tctaccagtt ctggatcaac acagcggatg
    38221 ccgacgttta ccgcttcctg aagttcttca ccttcatgag cattgaagag atcaacgccc
    38281 tggaagaaga agataaaaac agcggtaaag caccgcgcgc ccagtatgtg cttgcagaac
    38341 aggtgacacg tctggtgcat ggtgaagaag gtttgcaggc ggcaaaacgt attaccgaat
    38401 gcctgttcag cggttccttg agtgcgctga gtgaagcgga cttcgaacag ctggcgcagg
    38461 acggcgtacc gatggttgag atggagaagg gcgcagacct gatgcaggcg ctggtcgatt
    38521 ctgaactgca accttcccgt ggtcaggcgc gtaaaactat cgcctccaat gccatcacca
    38581 ttaacggtga aaaacagtcc gatcctgaat acttctttaa agaagaagat cgtctgtttg
    38641 gtcgttttac cttattgcgt cgcggcaaaa agaattactg tctgatttgc tggaaataat
    38701 gcattaagtg gaaaggggga gtgagaaatc actccccctg gtttttatac agggaacatg
    38761 atgaaaaata ttctcgctat ccagtctcac gttgtttatg gtcatgcggg taacagtgcg
    38821 gcagagtttc cgatgcgccg cctgggcgcg aacgtctggc cgctgaacac cgttcaattt
    38881 tctaatcaca ctcaatacgg caaatggact ggctgcgtga tgccgcccag ccatttaacc
    38941 gaaattgtgc aaggcattgc cgccattgat aaattacaca cctgtgatgc cgtattaagt
    39001 ggctatctgg gttcggcgga gcagggtgaa catattctcg gtatcgttcg tcaggtgaaa
    39061 gcagcgaatc cgcaggcgaa atatttttgc gatccggtaa tgggtcatcc ggaaaaaggc
    39121 tgtatcgttg caccgggtgt cgcagagttt catgtgcggc acggtttgcc tgccagcgat
    39181 atcattgcgc cgaatctggt tgagctggaa atactctgtg agcatgcggt aaataacgtc
    39241 gaagaagcgg ttctggcagc gcgtgaactc attgcgcaag ggccacaaat tgtgttggtt
    39301 aaacacctgg cgcgggctgg ctacagccgt gaccgttttg aaatgctgct ggttaccgcc
    39361 gatgaagcct ggcatatcag ccgtccgctg gtggattttg gtatgcgcca gccggtaggt
    39421 gttggtgatg tgacgagcgg tttactgctg gtgaaactgc ttcagggggc aacgctgcag
    39481 gaggctctgg aacatgtgac cgctgcagta tacgaaatca tggtgaccac caaagcaatg
    39541 caggaatatg agctgcaagt ggttgctgct caggatcgta ttgccaaacc agaacattat
    39601 ttcagcgcaa caaagctctg aaatatctca tttcggccca caacgacctg tgggccgatg
    39661 gctattactt taagccttcc gctgacagcg cgtcttgtac ttccggacgt tcagccatac
    39721 gttgcataaa tgccgcaatg tgctctaacc cttccagatt cagtttcacc gcgtatgccc
    39781 agcgcagaac cgtaaacaga taggcatcag caattgtaaa tcgttgcccg cagatccagt
    39841 gctcatcctt cagtgcctcg ttcacatatt gcagcttctt ctccagctgc gcgcgaactg
    39901 tcgatttgta ctcttccggt gtatcagggc gaaacagagg tgtgaaacct ttatgcagct
    39961 cggtggcgat gtaattcagc cattcgatgg ttttatagcg ggaaatactg tttaccggtg
    40021 ccagcaactg gcggtcgggg acgctgtcgg caagatactg cataatcgct acgccttccg
    40081 tcagcaaagt accatcgtcc agcagcaatg caggtacctg tcccttaggg ttaacggcaa
    40141 agtaatcgtc accgttttcg agacgttttt tcattaaatc cacactgacg agcgtaaaat
    40201 cctttccgct ctcacgcagg gtgatatggg aagcgagaga gcaggcaccc ggtttgtaga
    40261 acaatttcat cggtaactcc ttttgtctgt ggtttaaagt atggtagtgc gttgctgaac
    40321 aaaaaaaaag ccgctaacga aaagttagcg gcttgtaaaa gagtttccct gaattttacg
    40381 ctacggctgc tttcgccgct ttgtctgcag cgtcatcctg cgtcatgcgg tgcagtttcg
    40441 gcgcggtcag cagcatcagt actgcaataa cggcagtagc gacaccaatc tgcaagaata
    40501 cgcgaccata gacttccagt gacatcagtg gatcggtaac gttatccggc acagccatca
    40561 tacccgcaac ataaccacca atcaggtttg caccggcagt ggtcaggaac cagctaccca
    40621 taatgaagcc catcagacgc tgcggaacga gttgtgcaac cattgccaga cccagaccag
    40681 agatcatcag ttctccgata ctctgcaggc catagcttgc gaccagccag cttacagaca
    40741 cgatacctgc gtcagatgcg aattttgcac ccagcggcag aatcaggaac gcaccagaac
    40801 acatcaccat accgattgca aacttagttg gcatcggcag ggtatcgccc atcttgttat
    40861 agatagcggc cagaatcggg ctaccaatga tgatccagaa cgggttcagt gcctgatact
    40921 gctcaggttc tacggccagg cccagaatgg tgtgttcaac gttacgaatc gcaaagaagt
    40981 tcagtgacgt tggcatctgg ctgtacagca cgaagaagat aatggcttcg agcatcagga
    41041 tgaaggcaac gatcatttta cgacgcgcag cacctttcat cgcgaaggct tctttaccga
    41101 agataaccac gataccaaag gcaacaacgc ccagcgccat acgcgcaact tcctgattgt
    41161 gcagcagcca ggtggcgata gcgatcagtg ccacaacacc aataatggtc agcagcaggt
    41221 tacggtagtt gataggctcg aagtctggtt ttgaaccgta ctgtttaacc cagcgttggc
    41281 agaaggcgaa gttaacgata gtgatcagca ggcctacaac gctcaacgca aacgcaacac
    41341 tccagccgta tttcgcggcc agccacgggg tggcgatcat agagaagaaa gagccgatgt
    41401 tgacggacat gtagtacatg gtgaatgcac cgtccagacg cgggtcgttt ttctcatagc
    41461 atgtagaaag cagagaagac gggttagctt taaacaggcc gttaccgacc gcaatagccg
    41521 ccatacccat ataaacgata ccggcgtcgt gaccagacca ggcaaccaga gcataaccaa
    41581 tcgccagcac aatagcgcca agcataatta cgcgtttagt acccagtacc ttgtcaccta
    41641 accagccgcc gatagcgacc agaccataaa ccagggcact aaaggaagag aaaagggtga
    41701 ttgaatccgc ttcagacata cccagttgtt taaccaggta aacagccata attccttgta
    41761 ggccgtaata accaaaacgt tcccataact caatcgagaa gatgagatag aacgccttcg
    41821 gttgtttgaa agcgttcaaa ctgacgcttt cagttggttt ttggtttgca gtggacactt
    41881 ttacctcttt ttttacatcc catattaacg ggggtgcttt ctcgcgttat gcctaaaaaa
    41941 catacgcgca attgttatag tgggagggga aacggcaggt aatgttcact atcttgcact
    42001 ttcagacaag tcctttgtaa taatctgtta catataactg aggcggggaa ttcaaccatt
    42061 ccgggaaatg ttatccagcg ttaaattttt caaaaaacgt aacggcagat tttttcacta
    42121 ccctgagagg gggatgatgg cttaacggcg atatgttctg ctatttagcg tttttcctag
    42181 tgagatagtc cagtttctga aaaatagcca gtgtaatgtt ttgtaggtca atatttgttg
    42241 cgtgtgtaaa tttaaatgat tttaacctag ttcttgcaat gtttttacat tgccacagca
    42301 tcgtgatctt gatcacgaat gaatagaaaa tttcgctatt aaaaaaacaa ttaacctgca
    42361 tttttggtta tccgcaaacg gattacctga tgataacgct aagcaatggc atcattgacg
    42421 ttaaaaagat gttttaaaag aggataaaga aaggttatca atggggtaat cggtgttacc
    42481 ccttttcttc agatgtcaac tttctctttg tattcacaaa gatcttcaat aatacaagag
    42541 ccacagcgag gcttgcgggc aatacaggta taacgcccgt gcaggatcaa ccaatggtgg
    42601 cagtcgactt taaactctgc cggaaccact ttcagtagct tttcttctac ctgttcgacg
    42661 tttttccccg gcgcaaattg agtacgatta caaacgcgga aaatgtgcgt gtcgacagca
    42721 atagtaggcc agccgaatgc agtgtttaat acgacgttgg ctgttttacg acctacacca
    42781 ggcagggctt caagcgcagc acgatcttcc ggaacctcgc cattatgctg ttccagtaag
    42841 atacggcagg ttttgatgat attttctgct ttgctgttat aaagcccaat ggttttgata
    42901 taggttttca ccccttcgac gcccagttca agcatcgctg caggtgtatt agccaccggg
    42961 tagagtttcg ccgtcgcctt attaacactg acatcggtcg cctgagcgga aagcagtacg
    43021 gcaatcagca attcaaaagg cgaactgaaa ttaagctcgg tggtgggatg aggattgttc
    43081 tcacgcaggc gagtgaggat ctccaggcgt tttgctttgt tcatcagaca ttccctgttt
    43141 caccgtttgg caatgcacgt tcggcagctg cttcagcacg gcgctttttc attctttcat
    43201 caatcaggta tttccctgcc agcatcagtc ccaggccaat aaatgcacct ggtggcagca
    43261 tcgccagcag gaaaggggag tcggtgtgga atatctccac gcgtaatact tttgcccagc
    43321 tacctaacag cgcatctgca ccgtcaaaca atgtgccatt gccgataatt tcgcgtagtg
    43381 aacccagcac gaacatggcg caggttgcgc ccataccaat tgaaaagccg tccagtgccg
    43441 aaagcgccgg accttttttg gcggcgaagg cttcagcgcg gcccacaacg atacagttag
    43501 tgacaatcag cggaataaaa atccctaatg attgatacag gccaaaggcg taggcgttga
    43561 tcagcatctg tacagcgctg accaccgagg cgatgatcat cacgtaaatg ggaatgcgga
    43621 tctcggctgg cgtccagtga cgcagcgtcg aaatggtcaa gttggtcagc gtcagtacca
    43681 gcgtagtcgc aagtcctaaa cccagagcgt tagtggcagt ggacgtgacc gccaatagcg
    43741 gacagaggcc gagcaactgg accagcgcag agttgttttt ccacaacccc tgaacaataa
    43801 cgtctttaat ttcgctcatg ctttattctc cacaggcagg aagttgagaa agttgtgccg
    43861 gtaacgtctg agcgtacaat ccggcgcgtt ttaccgcatt taccaccgcg cggggagtaa
    43921 tcgtcgcgcc ggtgaactgg tcgaaatcac caccatcttt cttcaccgcc cagtttgcat
    43981 catccgcacc actgattttt ttacccgcaa aatgggtgat ccagtcagaa aggcgcagtt
    44041 cgattttatc gccaagccct ggcgtttcgt ggtgctctgt cacgcgcgtg ccaagtaccg
    44101 tgccgttaaa atcggctccc accagcagct gaatcgcacc ggaatagcca tctggcgcgg
    44161 ttgtttccag aacggcggct accggtttgt catcctgttt ggcgatgtaa acccgatgct
    44221 cacctttacc taactctggc gcagtgacca gatagcaact ctgtgccagc gcattgttat
    44281 agcgttcggc tggcagcacc tgatcaaata acgccttttg ttgcagactg gcctgttcag
    44341 caattgtcgt tttggtcatc tggttgatgg ccgcagttaa ccctgtagaa cccgctgcaa
    44401 atagtgccag tgtaatgccg tgttttcgga tagttttcag catggtttac cctttgcgat
    44461 ggccgtagac gcgcggacgc gtgtagtaat cgatcagagg aaccgtgatg ttcgccagca
    44521 ggacggcaaa agccacgccg tcaggatagc cgccgaaact gcggatcaac cagactaata
    44581 aacccgccag cgcgccgaac atcagacgac cacgattggt cgtagaagca gtaaccgggt
    44641 cagtcaaaat aaagaatgcg ccgagcatag tcgcaccaga cagcagatga atttgcggtg
    44701 atgccagtgt ttctggtgag aacaaccagc ccaacgttgc gcataacgcc agcgttacta
    44761 agaagctgag gggaatatgc cagcgaatcg ctttctgcca tagcaaccac acgccgccag
    44821 ccagccaggc gagatttacc cattgccaac caacgcccgc cagcataccg ctgtagatcg
    44881 gatattgcat aatctgttca accgaatgac cggcacggac agaggtttta aatgtatcca
    44941 acggtgtcgc ctgactaatg ccatcaatac ctaagcgtag cgtgttcata tcaccaccac
    45001 tggtggtatg tccgctgaaa ataacctgta tggcgtcgat aaaaccaggg atgttgaccg
    45061 caatttcatg tggcggtaac cagctggtca tctgcaccgg gaaggagatc agtaagacca
    45121 cataaccaat cattgccgga ttaaacgggt tttgccccag accgccatac aactgtttag
    45181 caatgatcac cgcaaacacc gtacccagca cgaccatcca ccatggcgcg agggggggaa
    45241 tacttaccgc cagcaataag cctgtcagca atgctgagtt atctttcaac gttgcggcta
    45301 ccgactgctt gcgtagtttg agtacgagag cttcggctaa cagagcactg accgacgcca
    45361 acaggatctg aacgagagta ccccaaccaa aaaaccacag ttgcgctgcg attcctggca
    45421 cggctgcgag caacaccaac agcataatgc gcgatgtttg gcgctggtta tgggtataag
    45481 gggagctagc tattctgaat accatttagt cctcgtttac aaccttttgc tgggcggctt
    45541 ttttggcctg aacgcgggca atagccgcag caactgccgc tttgcgcgga tcaatctgtt
    45601 cttctggctc cgcattagcc tgttgctgtt ccagcttgcg tgctttggca cgggcaatag
    45661 cagcttcgac ggcggctttg cgcggatcaa cctgttcttc tggttctgcg ttagcctgtt
    45721 gctgttccag cttgcgcgct ttggcacggg caatagccgc ttcgacggca gctttgcgcg
    45781 gatcgacctg ttcttctggt tccgcattag cctgttgctg ttccagcttg catgctttgg
    45841 cacgggcaat agcggcttcg acggcggctt tgcgcggatc gacctgttgt tctggctcgg
    45901 cattagcctg ttgctgctcc agcttgcgcg ctttggcacg ggcaatagcg gcttcgacgg
    45961 cggctttgcg cggatcgacc tgttcttctg gttccgcatt agcctgttgc tgttccagct
    46021 tgcgcgcttt ggcgcgggcg atagcggctt caacggcagt tttacgtgga tcagcaacgg
    46081 ttgctgcgtc gttagtttgc tgcagttctg cctgtttcgc tctggcttgc gctttacggg
    46141 cttcccgtgc tgcaataatt gcactgttat ccgggcgttc gcccgcttta atcacaatag
    46201 gctgtgtagc ctgggcctgt ttctctttca cccgcgccag agcggcagca atcgcatctt
    46261 tatctttggc tgcaggttga acggctgcgc tcttatgtcg ttcaaggcga gccgcttttt
    46321 cgcgctccag acgagcctgg cgcgcttcga aacgcgcttt ggcttctgcg gcgcgctttt
    46381 cttcctgacg aatagctgca atttcagctt tttcctgacg gaaatattgc accaggggaa
    46441 tattgctcgg gcaaacccac gcgcaagccc cacattcaat gcagtcggca atgttatgcg
    46501 tggtagcttt atcgtgttgc tgacctttgc tgaaccagta caactgttgc ggcaaaagat
    46561 ccgcagggca ggcgtcagca caggcgctac accggatgca gctttgttct tcctgtggtt
    46621 cgccaagctc attggcagag ggagccaaca gacagttggt aatctttacg accgggacat
    46681 ccagccatgg caaggtaaag cccattagcg ggccgcccat aatcaccatt tgatcggcag
    46741 aggggcagaa tccggcgtca ttcaataaat gacgcactgg cgtccccagc cgtgcccaga
    46801 cgttgcccgg gcgagcgatt gcttcgccag tcagggttac gacacgctcg gtaatcggct
    46861 cgccatcaat aacggcacgt ttcactgcat aagcagtgcc gacgttttgc attaatacgc
    46921 cgatatcgga tgaacgcccg ccatgtggaa cctgcttccc ggtcagaatg taggttaatt
    46981 gtttagcacc gccagaagga tatttggttg gaatcacccg cagagaaata tcgtgagagt
    47041 ccgccagcac cgcgcgcagc atggaaatcg cctgcggttt gttatcttca atgccgataa
    47101 gaatttcgcg tggctgcaga atatgcgcaa gaatgcgaat accctctacg acctgagccg
    47161 cgcaatcctg catcaaacgg tcatcggcgg taatgtacgg ctcgcactca gcagcgttga
    47221 taatcaacgt ttcaatctta tctccgccac cctgcaattt aacgcctgtc gggaatcctg
    47281 caccgcccag cccggcaaca ccaaactgat gaatgcgctc gattaactct tcgcgactgc
    47341 gagagcgata atcggcccag ccgtctcgcg ggatccagca gtcttcacca tcggcatcaa
    47401 taatcacgct taattcagct aacgctgaag gatgagccgt agagtggggc gcaatagccg
    47461 taacggtacc cgaggtgggc gcgtgaacag gcagcatttt gccgcgacca cgggtaagcg
    47521 gctggccgcg caatacttta tcgccgacgc taacgcacaa ctcaccttca gcgccaatat
    47581 gctgtttcag tggaataaca aaacgctgcg ccagtggtac ctggcgcagg ggtgtaccgt
    47641 tggactgggt tttcatctcc ggtggatgga tgccgccgtt gaaatcccag attttatttt
    47701 ttctgaatgc agagaataac ttaagcatgg tgttccacgg gaatgatacg cacgggaatg
    47761 gtgttcagat cccatttcca ggagtcaggt gtttctgcga ccggttgcaa cgagatgcag
    47821 tgcgtcgggc acggatcaac acataaattg cagcccgtac agagatcact cattaccgta
    47881 tgcatggcac gggtagcgcc aacgatggcg tctaccggac aagcctgaat acatttagtg
    47941 cagccaatac agttattttc atcaataacc gccaccatcc gcgcaggcgt tagctcttgc
    48001 gcttcgccat ccagcggctg cggctcgaca ttaagcaact cggcaatttt tagcatcaca
    48061 gcttcgccac caggggcgca acggttgatt ttttcaccgt tatagctgat ggcttccgcg
    48121 taggggcgac agccgggata accgcactga ccacactggc tctgcggtaa gatttcgtca
    48181 attttctcaa cgaccggatc gtcttccacc gcaaaacggc gggaggcata acccagaatg
    48241 gcgccaaacg ccaggcccag caggctcacg gcggcaacgg caatccagat agcattcatt
    48301 acaacttcac caaaccacta aagcccataa aggccagaga cataagacct gcggtaatta
    48361 atgcaatggc attaccgcga aaaggtgccg ggacatcagc cacagcaagg cgttcgcgga
    48421 tggcggcgaa gagcaccatc accagcgaga aaccgacagc ggcggaaaaa ccgtacagcg
    48481 ccgactgaaa gaaattgtgc ccgagattga tattcagcaa cgccacgccg agcactgcac
    48541 agttggtggt gataagcggc aaaaaaatcc ccagcagacg gtaaagcacc gggctggttt
    48601 tgcgcaccac catctcggtg aactgcacaa ccacagcaat caccagaata aacgccaggg
    48661 tgcgcaggta aatcagatta agcgggatca aaatccacgt atcgataagc caggcgcaaa
    48721 tagacgccag cgtcatcaca aacgttgtag ccagccccat gcccatcgcg gtttccagct
    48781 ttttggaaac ccccataaac ggacagagac cgagaaactt gaccagtaca aagttattga
    48841 ccagtacagt tccgacaaac agtaacaggt agtcagtcat ttttcggcct gaaataaaaa
    48901 agccgcctat tatccgttaa tccagagcag gcgacaacag gttaactgta aggttattac
    48961 ggattcacaa acgtcgcttt cacccgcgac gagcgtttaa aataggggac gatcaatgcg
    49021 gttgccaaca aagtaaacag taactggcga acggctatgc catcttccac gggtgaaaaa
    49081 gcgaacgctt ttactgccag caatacggaa ataagcagcc agataatata gtgtttagga
    49141 acacaacgcc gacgtttgaa gaaagcgatg gtcaaccaca gtgtgtaata ccacatggcg
    49201 atagcggtaa taaaagaaac gccccataag atttgtgtcg tcagtgcttg ttcgccgagc
    49261 gtttgaaatg tttgcggaga agataacgca gcggtataca gcaacaacgc cagcgtcgta
    49321 cttaacagag cgactaaaag ccaggccaat gggccaagca accagcctcc aatacgttgt
    49381 ggcgttgtgg tggtcatagg ttctccataa tagcggcgca aacaggcaaa tctaatttta
    49441 ggggcgttat tataaggctt ttataaccga tcgctacttt ctttattgga catagtgcca
    49501 gacggacttc ggtacctggc cgaggtcaaa caaacgcccg ccagaaacca gctctgcgcg
    49561 acggtgatcg gcagcacgat acatattgat cagttcctga tcgtccgtca gggtgtaatt
    49621 aagatggtcg tagagttttt ccaaactttc gagtgaactg attttgcgaa attttaataa
    49681 gtagtcctga acagtcataa aaggtccata aaataattaa taccaaaggg gtaggtatta
    49741 ttagtgatga taa
//
LOCUS       DAZKGF010000011        49177 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZKGF010000011 DAZKGF010000000
VERSION     DAZKGF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.011"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..98
                     /gene="ymgJ"
                     /locus_tag="ACHOQV_000657"
     CDS             <1..98
                     /gene="ymgJ"
                     /locus_tag="ACHOQV_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFP9629078.1"
                     /translation="YQKRVIFYPTTLQRTAFKKCLFLMRDIIISS"
     gene            59..178
                     /locus_tag="ACHOQV_000658"
     CDS             59..178
                     /locus_tag="ACHOQV_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP9629079.1"
                     /translation="MPLSYAGYHYFIMMSLVSGEHNTLALPIQEQSPGLVPAG"
     gene            213..557
                     /locus_tag="ACHOQV_000659"
                     /pseudo
     CDS             213..557
                     /locus_tag="ACHOQV_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(922..1188)
                     /gene="minE"
                     /locus_tag="ACHOQV_000660"
     CDS             complement(922..1188)
                     /gene="minE"
                     /locus_tag="ACHOQV_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFP9629080.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1192..2004)
                     /gene="minD"
                     /locus_tag="ACHOQV_000661"
     CDS             complement(1192..2004)
                     /gene="minD"
                     /locus_tag="ACHOQV_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFP9629081.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2028..2723)
                     /gene="minC"
                     /locus_tag="ACHOQV_000662"
     CDS             complement(2028..2723)
                     /gene="minC"
                     /locus_tag="ACHOQV_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFP9629082.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3242..3610
                     /gene="ycgJ"
                     /locus_tag="ACHOQV_000663"
     CDS             3242..3610
                     /gene="ycgJ"
                     /locus_tag="ACHOQV_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFP9629083.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVN
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3713..4114)
                     /gene="pliG"
                     /locus_tag="ACHOQV_000664"
     CDS             complement(3713..4114)
                     /gene="pliG"
                     /locus_tag="ACHOQV_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFP9629084.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4356..4649
                     /locus_tag="ACHOQV_000665"
     CDS             4356..4649
                     /locus_tag="ACHOQV_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFP9629085.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4721..5380
                     /gene="ycgM"
                     /locus_tag="ACHOQV_000666"
     CDS             4721..5380
                     /gene="ycgM"
                     /locus_tag="ACHOQV_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP9629086.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5472..5918
                     /gene="ycgN"
                     /locus_tag="ACHOQV_000667"
     CDS             5472..5918
                     /gene="ycgN"
                     /locus_tag="ACHOQV_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFP9629087.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6125..6451)
                     /gene="hlyE"
                     /locus_tag="ACHOQV_000668"
                     /pseudo
     CDS             complement(6125..6451)
                     /gene="hlyE"
                     /locus_tag="ACHOQV_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            6712..7131
                     /gene="umuD"
                     /locus_tag="ACHOQV_000669"
     CDS             6712..7131
                     /gene="umuD"
                     /locus_tag="ACHOQV_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP9629088.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            7131..8399
                     /gene="umuC"
                     /locus_tag="ACHOQV_000670"
     CDS             7131..8399
                     /gene="umuC"
                     /locus_tag="ACHOQV_000670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFP9629089.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(8444..8974)
                     /gene="dsbB"
                     /locus_tag="ACHOQV_000671"
     CDS             complement(8444..8974)
                     /gene="dsbB"
                     /locus_tag="ACHOQV_000671"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFP9629090.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(9120..10661)
                     /gene="nhaB"
                     /locus_tag="ACHOQV_000672"
     CDS             complement(9120..10661)
                     /gene="nhaB"
                     /locus_tag="ACHOQV_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFP9629091.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            10883..11602
                     /gene="fadR"
                     /locus_tag="ACHOQV_000673"
     CDS             10883..11602
                     /gene="fadR"
                     /locus_tag="ACHOQV_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFP9629092.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(11654..13186)
                     /gene="ycgB"
                     /locus_tag="ACHOQV_000674"
     CDS             complement(11654..13186)
                     /gene="ycgB"
                     /locus_tag="ACHOQV_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFP9629093.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            13516..14814
                     /gene="dadA"
                     /locus_tag="ACHOQV_000675"
     CDS             13516..14814
                     /gene="dadA"
                     /locus_tag="ACHOQV_000675"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFP9629094.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            14824..15894
                     /gene="dadX"
                     /locus_tag="ACHOQV_000676"
     CDS             14824..15894
                     /gene="dadX"
                     /locus_tag="ACHOQV_000676"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFP9629095.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPYARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(15951..17687)
                     /gene="cvrA"
                     /locus_tag="ACHOQV_000677"
     CDS             complement(15951..17687)
                     /gene="cvrA"
                     /locus_tag="ACHOQV_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFP9629096.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(17782..18696)
                     /gene="ldcA"
                     /locus_tag="ACHOQV_000678"
     CDS             complement(17782..18696)
                     /gene="ldcA"
                     /locus_tag="ACHOQV_000678"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFP9629097.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            18796..19407
                     /gene="emtA"
                     /locus_tag="ACHOQV_000679"
     CDS             18796..19407
                     /gene="emtA"
                     /locus_tag="ACHOQV_000679"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFP9629098.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(19409..20143)
                     /gene="ycgR"
                     /locus_tag="ACHOQV_000680"
     CDS             complement(19409..20143)
                     /gene="ycgR"
                     /locus_tag="ACHOQV_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFP9629099.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNNAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            20344..20598
                     /gene="ymgE"
                     /locus_tag="ACHOQV_000681"
     CDS             20344..20598
                     /gene="ymgE"
                     /locus_tag="ACHOQV_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9629100.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(20648..22618)
                     /locus_tag="ACHOQV_000682"
     CDS             complement(20648..22618)
                     /locus_tag="ACHOQV_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP9629101.1"
                     /translation="MRLKKRYLCTALSLAFTQQALAAQESDTLTVWSSPVSSTTTTVL
                     DHPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(22644..23498)
                     /gene="modD"
                     /locus_tag="ACHOQV_000683"
     CDS             complement(22644..23498)
                     /gene="modD"
                     /locus_tag="ACHOQV_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFP9629102.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFHRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     NPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            complement(23495..24307)
                     /locus_tag="ACHOQV_000684"
     CDS             complement(23495..24307)
                     /locus_tag="ACHOQV_000684"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9629103.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMSQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVVELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            complement(24317..25075)
                     /locus_tag="ACHOQV_000685"
     CDS             complement(24317..25075)
                     /locus_tag="ACHOQV_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9629104.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGQ
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            complement(25072..26052)
                     /locus_tag="ACHOQV_000686"
     CDS             complement(25072..26052)
                     /locus_tag="ACHOQV_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9629105.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVTV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(26052..27074)
                     /locus_tag="ACHOQV_000687"
     CDS             complement(26052..27074)
                     /locus_tag="ACHOQV_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFP9629106.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCPLSDADY
                     QKLVAS"
     gene            complement(27411..29108)
                     /gene="treA"
                     /locus_tag="ACHOQV_000688"
     CDS             complement(27411..29108)
                     /gene="treA"
                     /locus_tag="ACHOQV_000688"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP9629107.1"
                     /translation="MKSPAASRPLKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQNEAEPTP"
     gene            complement(29429..30847)
                     /gene="dhaM"
                     /locus_tag="ACHOQV_000689"
     CDS             complement(29429..30847)
                     /gene="dhaM"
                     /locus_tag="ACHOQV_000689"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP9629108.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADTDHVLVMMDIGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPSYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFSRQG"
     gene            complement(30858..31490)
                     /gene="dhaL"
                     /locus_tag="ACHOQV_000690"
     CDS             complement(30858..31490)
                     /gene="dhaL"
                     /locus_tag="ACHOQV_000690"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP9629109.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSIPAALEAASS
                     VAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(31501..32571)
                     /gene="dhaK"
                     /locus_tag="ACHOQV_000691"
     CDS             complement(31501..32571)
                     /gene="dhaK"
                     /locus_tag="ACHOQV_000691"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP9629110.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRSDAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEESQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            32799..34718
                     /gene="dhaR"
                     /locus_tag="ACHOQV_000692"
     CDS             32799..34718
                     /gene="dhaR"
                     /locus_tag="ACHOQV_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFP9629111.1"
                     /translation="MSSAFNNDGRDISPLIATSWERCIKLMKRDTWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRACALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQEDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLHSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            complement(34755..34931)
                     /locus_tag="ACHOQV_000693"
     CDS             complement(34755..34931)
                     /locus_tag="ACHOQV_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629112.1"
                     /translation="MKKTFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMP
                     PSNGGPNHGSHGRY"
     gene            complement(35122..36213)
                     /gene="ychF"
                     /locus_tag="ACHOQV_000694"
     CDS             complement(35122..36213)
                     /gene="ychF"
                     /locus_tag="ACHOQV_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP9629113.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEEREEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(36330..36914)
                     /gene="pth"
                     /locus_tag="ACHOQV_000695"
     CDS             complement(36330..36914)
                     /gene="pth"
                     /locus_tag="ACHOQV_000695"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP9629114.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEIWFTDGLTKATNRLHAFKAQ"
     gene            37192..37470
                     /gene="ychH"
                     /locus_tag="ACHOQV_000696"
     CDS             37192..37470
                     /gene="ychH"
                     /locus_tag="ACHOQV_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFP9629115.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(37525..39204)
                     /gene="dauA"
                     /locus_tag="ACHOQV_000697"
     CDS             complement(37525..39204)
                     /gene="dauA"
                     /locus_tag="ACHOQV_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFP9629116.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLMSGVFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(39329..40276)
                     /gene="prs"
                     /locus_tag="ACHOQV_000698"
     CDS             complement(39329..40276)
                     /gene="prs"
                     /locus_tag="ACHOQV_000698"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9629117.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(40427..41278)
                     /gene="ispE"
                     /locus_tag="ACHOQV_000699"
     CDS             complement(40427..41278)
                     /gene="ispE"
                     /locus_tag="ACHOQV_000699"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP9629118.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGAEISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(41278..41901)
                     /gene="lolB"
                     /locus_tag="ACHOQV_000700"
     CDS             complement(41278..41901)
                     /gene="lolB"
                     /locus_tag="ACHOQV_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFP9629119.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPSNMELTDGGQRIKLKMDNWIVK"
     gene            42115..43371
                     /gene="hemA"
                     /locus_tag="ACHOQV_000701"
     CDS             42115..43371
                     /gene="hemA"
                     /locus_tag="ACHOQV_000701"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFP9629120.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDGLGLE"
     gene            43413..44495
                     /gene="prfA"
                     /locus_tag="ACHOQV_000702"
     CDS             43413..44495
                     /gene="prfA"
                     /locus_tag="ACHOQV_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP9629121.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            44495..45328
                     /gene="prmC"
                     /locus_tag="ACHOQV_000703"
     CDS             44495..45328
                     /gene="prmC"
                     /locus_tag="ACHOQV_000703"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9629122.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVALAQRNAQNLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIHAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            45325..45717
                     /gene="sirB2"
                     /locus_tag="ACHOQV_000704"
     CDS             45325..45717
                     /gene="sirB2"
                     /locus_tag="ACHOQV_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFP9629123.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            45721..46530
                     /gene="sirB1"
                     /locus_tag="ACHOQV_000705"
     CDS             45721..46530
                     /gene="sirB1"
                     /locus_tag="ACHOQV_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFP9629124.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            46566..47420
                     /gene="kdsA"
                     /locus_tag="ACHOQV_000706"
     CDS             46566..47420
                     /gene="kdsA"
                     /locus_tag="ACHOQV_000706"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFP9629125.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(47569..47676)
                     /gene="ldrA"
                     /locus_tag="ACHOQV_000707"
     CDS             complement(47569..47676)
                     /gene="ldrA"
                     /locus_tag="ACHOQV_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9629126.1"
                     /translation="MTLAQFTVIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(48081..>49177)
                     /gene="chaA"
                     /locus_tag="ACHOQV_000708"
     CDS             complement(48081..>49177)
                     /gene="chaA"
                     /locus_tag="ACHOQV_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFP9629127.1"
                     /translation="NAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINLLA
                     LIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTLMR
                     DTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMALP
                     AANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAHSS
                     LWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGALKA
                     VLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLVLC
                     HISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT        12246 a        12204 c        12711 g        12016 t
ORIGIN      
        1 attatcagaa aagagtgatt ttttatccaa ctacacttca gcgcactgcg tttaaaaaat
       61 gcctctttct tatgcgggat atcattattt catcatgatg tctttggtga gcggtgaaca
      121 caatacactt gcgctgccta ttcaggagca atcccctggt ctggtgcctg cgggatgatg
      181 ttgcagcaga gctaaggtca cttagacgct actatcattt atccaatggc atggggtcga
      241 aatcagtcga tacccgcagt atatatcgtg aacttggtgc aacgctgagt tacaacatgc
      301 gtctggggaa cggcatggaa gttgagccgt ggctgaaggc ggctgtgcgc aaagaatttg
      361 tcgatgataa ccgggtgaaa gtgaataatg acggtaattt tgtcaatgat ttgtcgggca
      421 gacgtggaat atatcaggca ggtattaaag cctcattcag cagttcgtta agcgggcatc
      481 tcggggtggg gtatagccat ggtgccggtg tggaatcccc atggaacgcg gtggctggtg
      541 cgaactggtc gttctgacca tcaacgatta aactgcgctt cgcgcagttt tcgtttaccg
      601 ggtgctaaaa gggaaaattc tgcggcaaaa aaagcccgcc agttacggcg ggaaacctca
      661 tcctatggga gaacaatgaa taatgaaatt gcggggttat catctcccag tatacccata
      721 ctaacaataa ggttatttac tcagccaggt ataaacaatt tgttctgtgt ggggggaacg
      781 gcattaagat gtaaaggggg ggaagcaatg agtagcatca gcaagaatac tcgccgctct
      841 tatcaaacgt ggatgagggg gatgaaaaac tcaaggcaga gatgactctg ccttgaagat
      901 aaatgcgctt ttacagcggg cttatttcag ctcttctgct tccggtaagg tcacgttcag
      961 ctcaagaata gaaatatcgc catctttttg ctcaagctgt acggttacca tctcaggatc
     1021 aatttgtacg tatttacaaa tgacctcaag aatatcttta cgcaactgcg gcagataatg
     1081 cggttctgca tcactgcgac ggcgttcagc aacaataatc tgcagccgtt cttttgcaat
     1141 gttggccgtg tttttcttcc gcgatagaaa gaaatcgagt aatgccataa cttatcctcc
     1201 gaacaagcgt ttgaggaagc ctttcttctc ttcttcaatg aagcggaaag gacgttcttc
     1261 tcccaacaga cgttctacgg tatctgcgta ggctttaccc gcatcggcgt taatgtcgag
     1321 aatgaccggt tcaccctggt tagaggcgcg caatactgat tgatcctcag ggatcacgcc
     1381 gacgagtttg atgcgcagga tctccagcac atcttccatg ctcagcatgt cacctctgct
     1441 tacgcggcct gggttatagc gcgttaacag caggtgctct ttaataggct cttcgccatt
     1501 ttctgcgcgg cgtgatttcg acgccagaat gcctaaaata cggtcagagt cgcgtactga
     1561 ggagacttcc gggttggtgg taataatggc ttcgtctgca aaatagagtg ccattagcgc
     1621 accggtctca atccctgccg gggagtcgca aacaataaat tcaaagtcca tcgctttcag
     1681 atcatcaaga actttggcga ccccttcacg ggtgagggca tctttatcgc gagtttgcga
     1741 tgccggaaga atatagagat tttcagtacg cttatcttta attaacgcct gatttagcgt
     1801 tgcatcgccc tgaatgacgt tgacgaaatc gtaaacgacc cgacgttcac aacccataat
     1861 caggtcgaga ttacgcaggc cgatatcaaa atctatcacg acagttttct ttcccttctg
     1921 ggccaaacca gtggcgatgg ccgcgctgga ggttgtctta ccaacacccc ctttgcccga
     1981 agtaacaaca ataatgcgtg ccatagaaat tccttgttaa aaagggatca atttaacggt
     2041 tgaacggtca aagcgttttc gactaactgc agtcgcgccg ctttgccata aaattctgct
     2101 gggatttgat cactcagcca gtattcacct gcgatggaca ccagttccgc catcaggttc
     2161 gtacaaaata tttgcgtttc ccggtcacca cttgcccctg ccagcgcacg accgcgcatc
     2221 atgccataga cgtgaatatt accatcggca atcaattcgg ccccagcgct aacgtggctt
     2281 gtaacaatca gatcacattg tggagcataa atacgctgac cggaacgcac cggggtatct
     2341 attaaacgcg tttttgtgac cggcgttgta ttttgcgctg gagcctgcgg tgcgggagct
     2401 ggacgcggcg ctttttcctt tccttccgtc aggataggca gccccatctt ttcaatttcg
     2461 gctttaagtt gcgcatcttt gcagccactt acgccaataa cccgcaaacc ggttgccgaa
     2521 accgccttat gcatcgctga ccagtttacc gggtcttcca gtgcactgac gttgagtaca
     2581 acgggggcat gttttaaaaa tgcgggggcc tgagcgattt tgtcttccag cgcctgatgg
     2641 ataaccttag gttctgcctc atgcagatga accacagata aagtgaagct actgccttta
     2701 agctcgattg gcgtgtttga catcctggcc ttactcaatt agctattaat catcgccagc
     2761 gcgcgatgat gttccgaaga ctataaggca tgttatagtc tggattatat tgaggcaagt
     2821 caccctccca tttattcaga gtaaaagtct attctgtgat aaatggcgct gattcatagc
     2881 ttaaaaaata cccttgtcaa tcaacccatt gccgtcgtac ttttgattgt tcttatttac
     2941 gcttcctttt tccgcaccct aatcgtgaca gcatcttcag acctgtttaa atatccagta
     3001 ttgaggcgag tgtatttttt caccacgcca ggctttctta actttccatt tttgcagcat
     3061 cattaaagaa cagatacccg ttatcttaat atccgccaca gaattgctct atcatagaaa
     3121 ccgcagaaga cgtctgatac ttctgtcagg tcatcgtaac tcgtccaggt cagcggtttt
     3181 gattacggat tgatgatgaa atgagcataa gtgttgtacg gttggattat caggatgtgc
     3241 tatgaatatg atgcgtattt tttatatcgg attgtcaggt gtggggatga tgttctcatc
     3301 aatggcgtct ggacatgacg ctggcggact tcaatctccg gcgtgcggcg ttgtttgcga
     3361 cccgtatatt tgtgttaact cagatggcat ttccccagag ttaacaagga aatatctcgg
     3421 cgaaaaagcc gctgaaaact tacaatcatt acaaggctac gatcccagcg aatttacatt
     3481 cgctaacggt gtattttgcg atgttaaaga aaaattatgt cgtgatgatc gttattttgg
     3541 tgtgaatggt aagcggagtg gaaaaatcaa tcaaaccacc acaaagatgt tatttatgtg
     3601 tcgtgagtga tgtcttgttt acattatgtt ctattcgtca ttaacctctt gagcgacaga
     3661 gaggagtggg gcgttaacgt ttatgacggg gagagtcccc gtcggttgat atttacttaa
     3721 tttgaatatc gacgttatat tttttagttt tgtttttacg ggcgtcgttt cgggtttgca
     3781 gcactcgcaa ctcatatttg ccagaagcag ggagtgagta ttgtccgtga ctatcgagtt
     3841 ctggggaata tctggataaa tcaacagaat catcgattcc tggcccgaag aggtaagtat
     3901 cagcgccttc attagagata ctcacatgca ctttctgacc ttttttggca tagaaggtat
     3961 atgtatcgta atcgtatccc tttatttcgc cggagtactg agcgctgcta tggccttttc
     4021 tgaactcaac attgacattc ttacccgcag caaagctcgt ggaggttagc agtgcaagca
     4081 acaatacagc cttactgatg cccttaattt tcattggtag tctcctgttc agaatgagta
     4141 cgatgaactg ttataatata acaattcatc acaggagaat tccagtggct ttcctgcgtt
     4201 cggttaaatc ccggcgcggc atttttgccg gggggattac ttataaaggg ggcatggact
     4261 gaacaaaccg taggaatcta cagctggctg gcttgtggcg cgggtttcat tgatgttagc
     4321 ttatgccaaa acctggcatc ttaaagagta aaagtatgtt ttgtgtgatt tatcgaagca
     4381 gcaagcgtga tcagacctat ttatatgtcg aaaaaaaaga cgatttttcg cgtgttcctg
     4441 aggaactgat gaaaggtttt ggtcagcctc agttagcgat gattcttccg ctggatgggc
     4501 ggaagaaact ggtcaatgcc gatattgaaa aggttaaaca ggcattaacc gagcaaggtt
     4561 actatttgca gttaccgcca ccacccgaag atttgctgaa gcaacatctt tccgtcatgg
     4621 ggcagaaaac agacgacacg aacaaataac cgatatccgg cggtggcatt atctttgtcg
     4681 gcgcgggttt tcatatccac gataaggtga ggggaacgtt atgtatcaac atcacaactg
     4741 gcaaggtgcg ctgctggatt atccggtgag taaagtagtc tgtgttggta gtaactatgc
     4801 caaacatatt aaagagatgg gcagcgcagt gcccgaagag ccagtgctgt ttattaaacc
     4861 agaaacggca ctgtgcgatc tgcggcagcc attggcgatc ccatccgatt tcggttcagt
     4921 tcatcatgaa gtcgaactgg cggtattgat tggcgcgacg ctgcgtcagg ctacggaaga
     4981 gcatgtccgt aaagccattg ccggttacgg cgtggcgctc gatctgacat tgcgtgatgt
     5041 tcagggaaaa atgaagaaag ccgggcagcc gtgggaaaag gccaaagcgt ttgataactc
     5101 ttgtccgctt tccgggttta ttcccgcggc ggaattcacc ggcgatccgc aaaatacaac
     5161 gctgggcctg agcgtaaacg gcgaacaacg ccagcaaggt acgactgcgg acatgatcca
     5221 taaaatcgtt ccgctgatcg cttatatgag caagttcttt accctcaagg ccggtgacgt
     5281 tgtgctgaca ggcacgcctg atggcgtcgg cccgttgcaa agtggtgatg agctgacagt
     5341 cactttcgat gggcattctt tgacaactcg cgttttgtaa tactttttgc cgcctgaaag
     5401 cggcggcaaa acttgcatcg ctgtgccaga ctggttataa ggtgcgtttt aacgtaatgg
     5461 cggaacacct gatgagcgat gtacctttct ggcaaagtaa aaccctggac gaaatgagcg
     5521 atgcggaatg ggagtcgttg tgtgatggtt gcggtcagtg ttgcctgcat aagctgatgg
     5581 atgaagacac cgacgaaatc tacttcacta acgtcgcctg tcgccagctc aatattaaaa
     5641 cctgtcaatg tcggaactac gaacgtcgtt tcgagtttga acctgactgc attaaattaa
     5701 cccgtgaaaa tctgccaaca ttcgaatggc tgccaatgac ctgcgcttat cgcttgctgg
     5761 cggaaggtaa agatttacct gcgtggcatc cgctgcttac tggttcgaaa gcggcaatgc
     5821 atggtgaacg tatctctgtg cgtcatatcg cagtgaaaga atcagaagtg attgactggc
     5881 aggatcatat cttaaataaa cctgactggg cgcagtgata aatttaatag atgttgatgg
     5941 gggctttctt aatatacgtc tggatactcg ccatttcgta gccctgtttt tgaaagcgct
     6001 ctgtcataat gagagttacc cgatacctta ttatcaacag gtaactctca atctttaaaa
     6061 aacggctgtg tttttaaatg tgataaacct tataccttga gtacatggag agaataatcg
     6121 cttatcagac ttcaggtacc tcaaagagtg tctttttacc gtgtcttttc tgatactcat
     6181 tacaggtgtt aatcattttt ttggccgctt cttttagcaa agaaagcatt aaatcatcat
     6241 aatcaacata gaatctggtt gtttcagttt ccgtttttat ctccccgatg gcggctattt
     6301 cggtggttaa tttcaatttg gcggcatcga tatctttatt cgcttgttta accgtgttag
     6361 acagggtggt aaagaaactc tgcacagatt ttaacttgtt cttcaattct ggaatcagtt
     6421 tcccttcaac tacgcccgct gcaacagaat acagtttctt gatgccgtca tccagtacct
     6481 taatgagaat gcctttctgg gcgaatgctt ctattctatt ttttagccgg gcgatatttt
     6541 tcatttctgc tggatgagcg tcgtcgccag aaggccacgt gagcacagga taagagaacg
     6601 aaaaatcagc agtctatgca gcgacaaata ttgatagcct gaatcagtat tgatctgctg
     6661 gcaagaacag actactgtat ataaaaacag tataacttca ggcagattat tatgttgttt
     6721 atcaagcctg cggatctccg cgaaattgtg acttttccgc tatttagcga tcttgttcag
     6781 tgtggctttc cttcaccggc agcagattac gttgaacagc gcatcgatct gaatcaactg
     6841 ttgatccagc atcccagcgc gacttacttc gtcaaagcaa gtggtgattc tatgattgat
     6901 ggtggaatta gtgacggtga tttactgatt gtcgatagcg ctattaccgc cagccatggt
     6961 gatattgtca tcgctgctgt tgacggcgag tttacggtga aaaaattgca actacgcccg
     7021 acagtgcagc ttattcccat gaacagtgcg tactcgccca ttaccatcag tagcgaagat
     7081 acgctggatg tctttggtgt ggtgatccac gtcgttaagg cgatgcgctg atgtttgccc
     7141 tctgtgatgt aaacgcgttt tatgccagct gtgagacggt gtttcgccct gatttatggg
     7201 gtaaaccggt ggttgtgcta tcgaataatg acggttgcgt tatcgcccga aacgctgagg
     7261 caaaggcgct gggcgttaaa atgggcgatc cctggttcaa acaaaaagat ctgtttcgtc
     7321 gctgtggcgt ggtttgcttt agcagcaatt atgagcttta cgcagacatg agtaatcggg
     7381 tgatgtcgac gctggaagaa ctatcgcccc gcgtcgagat ttacagtatt gatgaggcat
     7441 tctgcgatct gaccggtgtg cataattgtc gcgatctgac tgattttggc agagaaattc
     7501 gcgcaacggt gctacaacgt acccatctta ctgttggtgt ggggatcgcc cagaccaaaa
     7561 cgctggctaa gcttgccaat catgcggcaa aaaaatggca gcggcagacg ggcggggtgg
     7621 tggatttatc aaatctggaa cgccagcgta aattaatgtc tgctcttccc gtggatgaag
     7681 tctgggggat tggacggcgg atcagcaaaa aactggacgc gatggggatc aaaaccgttc
     7741 tcgatttggc ggattcagat atccggttta tccgtaaaca ttttaatgtc gtgctcgaaa
     7801 gaacggtgcg tgaactgcgc ggtgaaccct gtttgcaact ggaagagttt gcaccgacga
     7861 agcaggaaat tatctgttcc cgctcgtttg gtgaacgcat cacggattat ccgtcgatgc
     7921 ggcaggccat ttgtagttac gctgcccggg cggcggaaaa acttcgcagc gagcatcaat
     7981 attgtcggtt tatctccacg tttattaaga cgtcaccatt tgcgttcaat gaaccttatt
     8041 acggcaatag cgcatcggta aaactgctga cgcccactca ggacagcagg gatatcatta
     8101 acgccgctac gcgatctctg gatgccatct ggcaagcggg ccatcgctac caaaaagcgg
     8161 gcgtgatgct gggggatttc ttcagtcagg gagtcgcgca gctcaattta ttcgatgaca
     8221 acgcaccgcg ccccgggagt gagcaattga tggcggtaat ggatacgctg aatgctaaag
     8281 agggcagagg aacactctat tttgccgggc aggggatcca gcaacaatgg cagatgaagc
     8341 gtgccatgct ttcaccatgt tatacaacgc gaagttctga tttactgcgg gtcaaataaa
     8401 catagcggca ggaaaaagcg ctcccgcagg agcgccgaat ggattagcga ccgaacaggt
     8461 cacgtttttt cgctttaaac ggctgggaaa tcaccaccag caccgcgaca atcaggtaag
     8521 cgataaaaat accgagcagc cactgcggca tttccagacc taaaaattcc cactgacgct
     8581 cggcgcaatc gccagaggcg acaaacactt gcggcaccca tttatccagc ggcagccatt
     8641 ccgggaaacg agccataaaa tcacaggtcg caaacggcga aggatagagc tgaagcatgg
     8701 tgtgctcgta agttaactgc acaccgcgga acgcactata caaccagata accatcgcta
     8761 cataacgcag cggagttttc ggggcgatcg cgccaatcag cgcagcaccc agaacgccga
     8821 ataatgcgca gcgttcataa atacagagca cgcaaggttt cagtaacatc acatgctgga
     8881 accacagtgc cgtcagttcc agtgctagag cggtaaacgc catcaacagc cacgcgcccc
     8941 ggccttgtga acattggttc aaaaatcgca acataatcat ttccctgcaa tatgcataga
     9001 gtgcgcagtt taaaccaatt cattcgctgc gccaccagga ggcagcaaaa ataggacgca
     9061 attggacaat tatcgggcga aaaggcaaac cgggcattta tatgcccggt aagttgttat
     9121 caaagcgttg ctatccagcc catttgcata aaccattcgg ttacaggggc aagcgtaaac
     9181 tcgacgcaga gcaagccgac gagtgtcagg acgagggtgt aaggcagggc catccacacc
     9241 atgcggccat aagagaggcg aatcaatggc gcgagtgcag aggtcagcag gaacaggaac
     9301 gcagcctgac cgttcggcgt agcgacggag ggcagattgg taccggtatt aatggcgacc
     9361 gccagcagct cgtattgctt caacgtgata gcgccacttt ccattgccgc tttcgcttcg
     9421 ttgatataaa tcgtccccac gaagacgtta tccgaaatgg atgacagcag accgttgaaa
     9481 atatagaaca gcgacagctg agcatgttcc gatgcctgca acacaaactg aataatcggc
     9541 gaaaacagtt gttggtcgat aatcaccgcg actaccgaga aaaacaccgt caacagtgcg
     9601 gtgaatggca gagactcggt gaaggcttta ccgatagcat gttcatcggt gacaccggtc
     9661 aatgatgtcg ccagaataat gaccgacaaa ccaatcaagc caacttccgc cagatgcaac
     9721 gccagcgcag tcaccagcca gacgccaata atcgcctgga caatcagacg gattttatcc
     9781 tgacgggtac gctggtggcg gctttgatcg tcaaactgtt gcagtacttc gcggactttc
     9841 tccggcagcg tttcaccata gccaaaccaa cgcagcttct ctaccagcag gcaggttaac
     9901 aggccacaaa tcagaaccgg aacggtcact ggcgacatgc gcaggaagaa atcgccaaaa
     9961 tgccagccag ccgctttagc gatgatcagg ttctgtggtt cgcccaccat ggtcatcacg
    10021 ccgcctaatg cggtgccgac accggcatgc atcatcaggc tacgcagaaa gccacggaac
    10081 tgttccagaa ccactttgta atgcttgtcg atatgactat cgtcttgcag gtcggtatct
    10141 tcggtacggg aagaggctac gcggtgataa ataccataaa aaccgactgc aacgctgatc
    10201 accaccgcca cgacggttaa ggcatcgagg aacgcggaga ggaacgccgc cgccacgcaa
    10261 aaagagagcg acagcagcat tttggagcga atgcttaaca gcaaacgggt aaatatgaac
    10321 agcaatagct gtttcataaa atagataccc gccaccataa acatcagtaa cagcaagact
    10381 tcaagatttg ccgccacctc ttcacggacg tgttccgcgc tggtcatgcc gatgaatacc
    10441 gcttcgatag ccaacagacc accggggagc agcgggtagc atttcagggc catcgccaga
    10501 gtgaaaataa attccgctac cagcaaccag ccagcgacga aagggctgat gaggaaaatt
    10561 aacggattta cgattaagaa aatgatgagg gcgagtttgt accagtcggg ggactggccc
    10621 aaaaaattgc gccatagtgc gcggccccag gagatctcca tgatggtttc ccttacctta
    10681 gaaataatca atgatgtttt tatgtttaaa cgcaaagctt aacggtcaga caggagtgag
    10741 gcaagtcttg atagtcaagg ggaaagagat gcagaaaatg aagccttgat cccttttttc
    10801 ttctttttgt cttctatcag catagttagc cctctggtat gatgagtcca actttgtttt
    10861 gctgtgttat ggaaatctca ctatggtcat taaggcgcaa agcccggcgg gtttcgcgga
    10921 agagtacatt attgaaagta tctggaataa ccgcttccct cccgggacta ttttgcccgc
    10981 agaacgtgaa ctttcagaat taattggcgt aacgcgtact acgttacgtg aagtgttaca
    11041 acgtctggca cgagatggct ggttgaccat tcaacacggc aagccgacga aggtgaataa
    11101 tttctgggaa acctctggtt taaatatcct tgaaacactg gcgcgactgg accacgaaag
    11161 tgtgccgcag cttattgata atttgctgtc ggtgcgtacc aatatttcaa ctatttttat
    11221 tcgcaccgcg tttcgtcaac atcccgataa agcgcaggaa gtgctggcaa ccgctaatga
    11281 agtggccgat cacgccgacg cctttgccga gctggattac aacattttcc gcggcctggc
    11341 gtttgcttcc ggcaacccga tttacggtct gatccttaac gggatgaaag ggctgtatac
    11401 gcgcattggt cgtcactatt tcgccaatcc ggaagcgcgc agtctggcgc tgggcttcta
    11461 tcacaaactg tcggcgttgt gcagtgaagg cgcacacgat caggtatacg aaacagtgcg
    11521 tcgctatggg catgagagtg gcgagatttg gcaccggatg cagaaaaatc tgccgggtga
    11581 tttagccatt cagggacgat aatcccttcc gtttaaaaag caaacccctc aaacgagggg
    11641 tttttcgttg tttttacaga tttcccattc tcggcgggca acgttccagc aactcgacgc
    11701 tgccgtcttc gttttgctgt tccagcatca catcaaatcc ccacaggcga tgcacatgct
    11761 tcagcacttc tttgcgcccc cgatccagcg gtgcgcgatt atgtggaatg taacgcagcg
    11821 tcagcgaacg gtcgccgcgc aaatccacgt tccagatctg aatattcggc tccagattac
    11881 ttaaattata ttgagacgat aaccggttac ggatctcccg ataaccttct tcattatgaa
    11941 tagcggaaat ctccagataa ttatgccgat cgtcatccag cacggtgaag aagcggaaat
    12001 cacgcatcac tttcggtgac aggaactggc tgataaaact ctcatctttg aaatcacgca
    12061 tcgcgaaatg cagcgtttcc agccagtcgg aaccggcgat atccgggaac cagtatttgt
    12121 cttcttccgt tggcgactga caaatccgtt taatatcctg gaacatggcg aacccgaggg
    12181 catatgggtt gatgccgctg taccacgggc tgttataggg cggttggaag accacattgg
    12241 tgtggctgtg caaaaactcc agcataaaac gttccgttac tttcccttca tcatacagat
    12301 ggttaaggat ggtgtagtgc cagaaggtcg cccagccttc gttcatcacc tgagtctgtt
    12361 tttgcggata aaaatactgg ctcaccttac gcacaatacg caggatttca cgctgccatg
    12421 attccagcag tggcgcattt ttctccataa aatagagcag gttttcttgt ggttcggacg
    12481 gatagcggcg cgcttcagca accgttttct cttcctcgcg cttcggcagg gtacgccaga
    12541 gcatattgac ctgactttgc agatactctt cgcgactctt ctgccgggct ttctcttctt
    12601 gcagcgagat tttttgcggg cgtttgtacc ggtccacgcc gtagttcatc agcgcgtggc
    12661 acgagtccag aagccgttct acttcatcaa cgccataacg ttcttcgcac tcggtaatat
    12721 atttacgggc aaaaatcaga taatcgacaa tcgaactggc gtcggtccag ctacggaata
    12781 agtaattgtt tttgaagaaa gagttatgcc catagcaggc atgagccatc accagcgctt
    12841 gcatggtaat ggtgttctct tccatcaggt aagcgataca cgggttagag ttaatgacga
    12901 tttcataggc cagtccttgc tgaccgtgct tatacagccg ttcagtctcg ataaactttt
    12961 taccgaatga ccagtgcgga tagttaattg gcatgccgac gctggagtag gcatccatca
    13021 tctgttctga ggttatcact tcaatctggt gcgggtaggt atccagccgg tagagtttcg
    13081 ccacccggtc tatctctgcc agataaacat ccagcaggtc gaacgtccag tcgggtccat
    13141 cgctcaaacg tgtggtgtcc ttattcatag aatcgatcgt cgccatacgc gcacctcatt
    13201 gttgtcggcg ctctctgtgt ggagcacctc atttcaagca tagaacacct gttaaaaacc
    13261 gcgtcgccgg agaatttttt tctttgcgat ttcttattat cagagtgcca ctaatccgct
    13321 tctgaacgga attttatgct ggataaaaag ggcgttcagc aggagatact aaagacgcca
    13381 tattgccgca gagtcaggga gatgtgagcc agctcaccat aaaaaagccg tatgttgaat
    13441 aatattttca actgagttat caagatgtaa ttagattatt attcttttac tgtatctacc
    13501 gttatcggag tggctatgcg agttgtcata ctgggaagtg gtgtggtagg cgttgccagc
    13561 gcctggtact taaatcaggc aggacatgag gtcaccgtca ttgatcggga gccgggggcc
    13621 gccctggaaa ccagtgccgc gaatgccggg caaatctccc ccggatatgc tgcaccgtgg
    13681 gcggcaccag gtgtgccttt aaaagcgatt aaatggatgt tccagcgcca tgcgcctctg
    13741 gcggttcgtc tcgacggtac gcagttccag ttgaaatgga tgtggcaaat gttacgtaac
    13801 tgcgacacca gccactacat ggaaaacaaa gggcggatgg tacgtctggc ggaatacagc
    13861 cgtgattgcc tgaaagcatt gcgcgccgaa accaatattc agtatgaagg gcgtcagggt
    13921 gggacgctgc aactgttccg taccgaacaa cagtatgaaa acgcgacccg cgatatcgcc
    13981 gtgctggaag atgccggcgt accgtatcag ctgctggaat ccagccgcct ggcggaagtg
    14041 gagcccgcgc tggcagaagt ggcgcacaaa ctgacgggcg gcctgcaact gcccaatgat
    14101 gaaaccggag actgccagct gtttacccag aatctggcgc ggatggcgga gcaggcgggg
    14161 gttaaattcc gctttaatac gcccgttgac caactgcttt gcgacggcga gcaaatctac
    14221 ggcgtgaagt gtggtgatga agtgattaag gccgatgcgt atgtgatggc gtttggttct
    14281 tactcgacgg cgatgctcaa aggcattgtt gatattccgg tgtatccgct gaaaggctac
    14341 tcgctgacca ttccaattgc gcaggaagat ggtgcgccgg tatccactat tcttgatgaa
    14401 acctacaaaa tcgccattac ccgtttcgat aaccgcattc gtgtcggcgg aatggcggaa
    14461 attgttggtt ttaacaccga gctgttgcaa ccgcgtcgtg aaacgctgga gatggtggtt
    14521 cgcgatctct atccacgcgg cggtcatgtt gagcaggcga ctttctggac tggcctgcgc
    14581 ccgatgacgc cagacggcac gccggttgtc gggcgtacac gctttaaaaa tctgtggctg
    14641 aataccggtc acggcacgct cggctggacg atggcttgcg gttccggtca gttgttaagc
    14701 gatctgctct cggggcgcac gccagcgatc ccgtatgaag atctaagcgt agcgcgctac
    14761 agccgtggat ttacgccatc acgcccgggc catttacatg gcgcacacag ctaaggaaac
    14821 gagatgaccc gtccgataca ggccagcctc gatctgcagg cattaaaaca gaatctgtcc
    14881 attgtccgcc aggctgcgcc gtacgcgcgc gtctggtcgg tggtaaaagc aaacgcttac
    14941 gggcacggta ttgagcgtat ctggagcgcg ctcggggcca ccgatggctt tgcattgctt
    15001 aatctggaag aggcaataac gttacgtgag cgcggctgga aaggaccgat cctgatgctg
    15061 gaaggatttt tccatgctca ggatctggag atttatgacc agcaccgcct gaccacctgc
    15121 gtacacagca actggcagct caaagcactg caaaatgcgc ggctaaaagc accgttggat
    15181 atttatctta aagtgaacag tgggatgaat cggttgggct tccagcccga tcgcgtgctt
    15241 accgtctggc agcagttgcg ggcgatggcg aatgttggcg aaatgaccct gatgtcgcat
    15301 tttgccgagg cggaacatcc tgatggaatt tccagcgcga tggcgcgtat tgagcaggcg
    15361 gcggaagggc tggagtgtcg gcgttcgttg tccaattcgg cggcgactct gtggcacccg
    15421 gaagcgcatt ttgactgggt tcggcctggc attattttgt atggcgcttc gccgtccggt
    15481 cagtggcgtg atatcgccaa taccggatta cgtccggtga tgacgctaag cagtgagatt
    15541 attggtgtcc agacgctaaa agcgggcgag cgtgtgggct acggcggtcg ctatactgcg
    15601 cgcgatgaac agcgaatcgg cattgtcgcc gcaggatacg ccgacggtta tccgcgccat
    15661 gcgcctaccg gtactcctgt tttagtggac ggcgtgcgca ccatgacggt ggggaccgtc
    15721 tcgatggata tgctggcggt tgatttaacg ccttgcccgc aggcggggat tggtacgccg
    15781 gttgagctgt ggggcaagga gatcaaaatt gatgatgtcg ccgccgctgc cggaacggtg
    15841 ggctatgagt tgatgtgcgc gctggcgtta cgtgtcccgg ttgtgacagt gtaacttgtt
    15901 atcgctggat gcgaccagcg tcgtatccag cgttacaaaa cacattaaac ttaagattcg
    15961 gcttcttcct ccgctacccg aacaccaatc ttcaacactt cattatcctc tttctcggcc
    16021 accgtccaga tcattccggc aaattctacc tgatcaccga caaccggtgc cgcgcctaac
    16081 aactgctgga caatttcacc cagcgtttgc tgcttatcac gatattctcg cccgtcttcc
    16141 aggccatata tcagcgccac atcagcatat ttggcactgg cttcgagaat gaagtcacca
    16201 aagaagcgtt gatctagcgc gaccggtggc gactggctga acagtttacc gagcgccggg
    16261 agatcgcgtt cccgaccaat tacacacaac acatcgcctt cgcgcagtcg ggtgctgccg
    16321 gtaggatgaa gcaactggtt atcacgaaac agtgccgcaa tacgcgtctc ttttggcata
    16381 tgcagatcgc gcagtgccgc gcccacgcac catttatcgg cactcaactg ataaacaaac
    16441 tgctcccacg gattttccgg atgaatatcc agaccaacgc gtgacaccgg acgtcccact
    16501 ggcggaacga ccactttggc ttttttcgcc gcccacgaga gtgatgtccc ctgcaagagt
    16561 agtgaaacca gaaccacaaa gaaggcgaca ttaaagaaca gtcgcgcatt ctccagcccc
    16621 gccatcatcg ggaacacggc caggatgatt ggcaccgcgc cgcgtaatcc cacccagctg
    16681 ataaacacgc gctcacgcag attgaagcca cggaacggta gcaatccggc aaataccgaa
    16741 agaggacggg cgaagaaaat catccatgcg gacaaaatga gcgccggaat ggcaataggc
    16801 agcagatcgc ttgggttaac caacagcccc agcaccagga acatggcgat ttgcgccagc
    16861 caggcgaggc cgtcgaaatt ttgcagaatg ccgtagcggt tgcgaatcgg ccgattaccc
    16921 agcagaaaac cgcacagata aacagccaga ataccgctgc cttccagcgc cgtggttaaa
    16981 gcaaaaatta aaataccgcc gcttaatgcc agcaatggat ataaaccggc gggcagggcg
    17041 atgcgattaa tcatttgcag cagtaaataa ccaccgccaa gaccgatgac aatcccgagg
    17101 ccaaattgct gcaaaatatc gaccacgaac atccagctga cactgctttc atgctgctgg
    17161 atcatcgcaa tcagggtaat cgtcagaaag accgccattg gatcattact gccggattca
    17221 atttccagcg tcgagccaac acgttcgtta agccccttac cacccagcag agaaaagacc
    17281 gctgcagcat cggtggagcc gacgatagcg ccgattaata atccttcaat caaatcaaga
    17341 ttaaacagcc acgccgccat catgccggtt aaaccagagg tgataagcac gcccagcgtc
    17401 gccagcgaca gtgccggtcc taacgccaca cgaaaggaac tggcctgagt gcgcatcccg
    17461 ccgtcgagca aaataatcgc cagcgccaga ttacttacca tgtaggcgaa gggataatta
    17521 tcaaacggga taccgccgac gccatcaacc cccgccagca tgccgatcgc cagaaaaata
    17581 accagaatgg gaatgccaag acgggaagaa aatgaactaa gtaaaatact gctggtgaca
    17641 aggatggatc ctaagatgaa aaggctaatt attgttgtgg catccaacgg tcggttactc
    17701 ctgattacgc tgtctcttat ataaacccta ccatattagc ggcagagaca gcgttttact
    17761 tagccctgaa gcgtgatttt tttacatttt aagaacagga tgaccgctaa tcgtcaactg
    17821 ggtgccttcc tgggtgttat tcagaatggc atgtgcgccc aacggcagtg taaccgtgcg
    17881 ttgttcatga ccaaaatcga ggccggtaat gagtggaatc gacaggcggg aacgcaaaaa
    17941 cgcgtacact gactccaggt tgtaacctgc gtcataatca ttaggcgtgc tgccgctaaa
    18001 gctaccgaga ataatcgcct tctgacgtgg caaaattcca gcatgataga gctgcaacag
    18061 catacgttcg acgcggaaag ggtgctcgtt aatatcttcc agcaccagaa taccgttctc
    18121 aatttttggc atccacggtg taccaatcag tgaaatcagc atcgcgagat tgcctcccca
    18181 cagcgtgcct tcggtctgac atgtcgggcc ttccccttgc cattcaatgg tgaaggtttc
    18241 attgcgtaac gccagccaga aatggtgctc ggtaaaggcg ttcagttcat ccgcgccaaa
    18301 gtttgctacc agcatggggc cgctaaaggt gatgacattg ccatgcgcca gaagaccgca
    18361 ctgaatggcg gtaaaatcgc tatgtccgca aatgagcaac gggtcatgtt gttggcgagc
    18421 caccagtgcc tgccagtcaa tatctgccag taaacgactg gcaccgtaac cgccgcgtac
    18481 agccagcacg atggtgttgg gggtagtcag tctggcaaga gaattgagat cttccagacg
    18541 ctccgtttcc gtacccgcaa aacgctcaca acgacgggca ataacctcga cgttattgac
    18601 ctgatgcccc gcgtcggtaa ggcgctggat accacgcagc gcggcgtgct gtttaatgca
    18661 gtaacccgat ggggcaatta agtgaaacag agacatggca attccttgct gacaacagaa
    18721 acgaaatgta tatcatgccg cttaggtgtg ccgttgtcac ctcaacggcg attccaggct
    18781 ataaggatag aagaagtgaa attgagatgg tttgcctttt tgattgtgtt attagcgggt
    18841 tgttcatcga agcatgacta tacgaacccg ccgtggaacg cgaaagttcc ggtgcaacgt
    18901 gcgatgcagt ggatgccaat aagccagaaa gccggtgcag cctggggcgt cgatccacaa
    18961 ttgatcacgg cgattatcgc tatcgaatcg ggtggtaatc cgaacgcggt gagtaaatcg
    19021 aatgccattg gtttgatgca gttaaaagct tcaacctccg gacgtgatgt ttatcgccgt
    19081 atgggctgga gtggtgagcc gacgaccagc gagctgaaaa atccggagcg taatatttca
    19141 atgggggcgg cttacctgaa tattctggaa accggcccgc tggcaggcat tgaagatccg
    19201 aaggtactgc aatatgcgct ggtggtgtca tatgctaacg gggcaggtgc gctgctacgg
    19261 actttctcgt cagatcggaa aaaggcgatc agcaaaatca acgatctgga tgctgacgag
    19321 ttcctcgatc acgtagcgcg aaatcatcct gcgccgcagg ctccgcgcta tatctacaaa
    19381 cttgagcagg cactggacgc gatgtaaatc agtcgcgcac tttgtccgct ttctcccggg
    19441 cttctcgctc gagagagaaa ataatccgct gtaattgccg ctccaccgtc gggctgacgt
    19501 taagaaaacg gaagctcaga cggggagtgg tgatggtttc attcttgcca tcaatcactt
    19561 tgcgctcgct gatggagatt aactgggcgt caaagtgaaa aacaccccat tgccccatgt
    19621 tgacttcaat ctgagcgaag cgcatgcctt cgtgcaatcc ggcaggcttt gctgtttcca
    19681 gtaatgcgcc catgccgcct aacgacaaat catacaggcg gaaacgtaac gtactgttat
    19741 ccgccagttt ggtctggcaa aaatatggcg gatggagtgg ggcggagatg cggaaatatc
    19801 ggcgtcgttg tacaaaccac aaggtgggag gcggtacggt aataaatgcc ggaagctgca
    19861 agtattcact ctgctgtagt tgttcaacag taaactcgac tttcgcaccc tgggtttcgg
    19921 cggtaatggt aatgtgctgc gcctttaaca cagcgttgtt atcttcggct tgactgccga
    19981 aatccagcac cagtttatcc ggggttattg ccagtatttt gctgatcagc tgcccgccat
    20041 tccaactgat acgcaaagga attgcggctt tgtgcaaatc gcgtaacacg cccaggacgg
    20101 ctaacggatt ttgtttcagg aactgctcat ggtaatgact cacgcggaaa aactcctgac
    20161 tgacaaactg tcttaggttt atcggtcaca gttaacaaaa cttaatacaa atgcgtgaat
    20221 attttttaca tgtcgttctt aaatcagccg acatacgccc agcatcgatc ccgcccttgc
    20281 ctatacttag agcgttgacg taagcatttc ttgcgtccga ttcatcgaaa cgagggcctg
    20341 aacatgggaa ttattgcctg gattattttt ggcctgatag ccggcattat cgccaagcta
    20401 atcatgccgg ggcgtgatgg tggtggattt ttcctgacct gtattcttgg gatagtcggt
    20461 gcggtggtcg gcggctggct ggcgaccatg tttggcattg gcggctccat cagcggtttt
    20521 aatttgcaca gcttcctggt ggcggtagtg ggcgctatcc tggtgctggg cgtattccgc
    20581 ctcctgcgaa gagaataaga ttttcataag gcggatagcg atacagatgc cgctatccgc
    20641 tttcacatca gaacgtatac tcgatacctg cccagaaatt gcgaccttct tcaataaacc
    20701 cttcactgta ggcatatttg gtatcaaaca ggttattgac cgacgcattg acgctgaagc
    20761 catgacctaa ggtgtaatcg gctcgaatgt gggtcaccgc aaaaccggcg gctttttgtg
    20821 aaccgtcact gttgctgtag ctggaggaac gcgcctcttc cgacagcgtt acgcttaacg
    20881 gctcccacgg tttgagagtc atccatgcgg tcattgtctg cgttggcaga tcggttatct
    20941 tgccgatgtc tttgcgtttg gcatcagcgt ggatcagggc gtagctcaat cctacatcca
    21001 gtatattgct gattttcccc ttaataccgg catccagacc gctgtaatcc accgtgccgc
    21061 tgttctgatt ttgaatggta tcggcatcga tattgtgcga gaggatggca tcactcaccc
    21121 ggttatagta aacgctgacc tcaaaccccc agtcgtgcgt gaaggcacca ttccaggtta
    21181 aatccacccc gcgcgcgcgt tccggtttga gctgcgggtt aacaatcgct atctggttat
    21241 acgcaggttt ggacgtggta tagcgttctt tcagcgtcgg aaagcgtgtg cggtcatagt
    21301 acgaaagcgc cagcgtgtct tcattggcaa agtggtattt ccccatcacc tgccagttaa
    21361 aagctgactg attgttgtcg tcataatggg tgatgcttcc atctttctca tgttttttcg
    21421 cttctacgct atcgcgccag tcatagctga ttccagccac gacatcgaca ttatcggcag
    21481 cagcccattg atattcactg gcgagcgacc aggtacgatc ttcatagcga tcgtaagcgg
    21541 cgtgcggcgc acctttttcc cggtgtacgt catctttcca gttaacggca aacgacagca
    21601 gatcgttttc acgcacatcg gctgccagtt gtaacccggc accgtcgctg taatcggaat
    21661 aatggctgta gctgcctttt ttatttttca aatcagccag cgagttgtac atcatcagcg
    21721 tattttcaaa ggtatcgcga tacagccgac ttttcagggt aaaacgatcg tttagttggg
    21781 tcgttccctg ataataaaaa ctttctttgt catactctgg ccactgccag tagcgtgatt
    21841 tttgaccact atttccactg tatggcggat tatctttttc accatcctgc ttaatgtaag
    21901 tcaatgtgta ttcatcgttt tcgcgtggtg taaaaccgag cttcacaatg ccgcgtttat
    21961 catcagccga tgaattaatc atcttgccgt gtttgcctgc aatatcatta tttacaccat
    22021 gcggcaggcc gagaaaatcc tgctttagct ggctaccgct gacttgcaaa taccccagat
    22081 cgctgctggc ggcaaatgaa gcatgcatat cgtaggcatt gtcctggcta cggctccatc
    22141 cctggcgata tcccagattt gcttccagag gttttgttgg cttctgggtg gtgatattaa
    22201 tggctccgcc catctgatta ggcccctgaa gcagcgacga ataccctttg gaaacttcaa
    22261 ctgcccccaa attgttggtc agaatccgcg ccagatcgag gttgccgtca tagggaacat
    22321 aaatgggcac accgtcgaaa tagactggca cctgacgact atcaaagcca cgaactttga
    22381 cctgttcttc gttgcggcta cctgactttt gcagcaccac gccggggacg acacttaatg
    22441 cctgagcgac attctgttta tccagggcct tcatggtggg gtgatcgaga acggtggtcg
    22501 tcgtcgatga taccggactg gaccatacgg tcagcgtgtc actctcctga gccgctaagg
    22561 cctgctgggt aaaggcaagt gataatgctg tgcagagata acgttttttg agtctcatag
    22621 aataaattcc gtttaacaaa gaattaaata gaggctgcgg gttgcagact gactttaatg
    22681 tcagctggtg tcgcgtaata aggcgcggag gtaataaaaa ggcgaatgcc gcagtcgagg
    22741 taatttttga gtgttgtcag attaattccg ccggtcagag ccagcgtgca gtgaggagcc
    22801 agcgacggtg ctatttgcgc aatttcagtt gcctgttgcg gattaaattt gtcgagctga
    22861 agtacgtctg gttgcgcgcg taacgcggcg attgcctctt tcggcgtgtc ggcttcaaca
    22921 actattttct tctcgggtgc gtggcggcgt aactgattga ttgcgcctga ccagtcctga
    22981 ttgtcatgaa gaaaatgacg gtggttggca aacaataata tggtttccgc acatccggca
    23041 cgatgaatca gtcctccggc agccagaatt gcctgcgagg ccagtaagcg ggtgcccgga
    23101 attgcttttc gggtgcaggc gatattgcca tcagggtaac gttcacgaag taacgccagc
    23161 atttgagcga gataatcaga aacaccgcaa ctccactcca gcacattctg gactgccttc
    23221 catccctgat gaagtgctgc ggcattaccc tgcgcacgga ttagccgctg acccgcgttc
    23281 gcttgtgaac cgtcgctgac tgcgtcatca atagttaatc ccagcgtagt taacatctta
    23341 cacgcgacag aaattccgct gacgcagccg ccctgacggt gaaaaaactc tatatagcca
    23401 tgctggtgtc caatatttaa tgctcgcgtg gtcaggtcac cgccctggat atcttccaga
    23461 agtaacgcat cgatttgtgc ctgagaaagg aaaatcatcg cagtgcctct tgcggtacgc
    23521 tatcccacca gatgagcgcc cagtcgcggc tggcaggggc gagggcacga gcatcttgtt
    23581 gttgatacca gcgatataac cgctcacgct catcgtcgtt aagcgcgccc agactccagc
    23641 tgacgttctg ctcaaaacgt tcccaggtgc tgttatcctg ttggcaattt tgtccgcgga
    23701 taaaatcaac gtgagcataa attcccatct gatacaggac gttgagcgca aagatatagt
    23761 tgggtaattc gaccacttcc cgaccagccg cccgctgaat ggctggagag acgaacgagg
    23821 tgctgaccag atgagtcgta tagacgcgaa gccgtgcctg attattcagt ttgctcattg
    23881 cctggctcat atcagccacc agagtggagc gggaagccac ggcaatatcg cagcgtggca
    23941 ggtcactcca gtcctcttcc caggcgcgct ggatccagtg gacattgtct gcctttagcg
    24001 cggcggcgcg acgggcggca acgttcagca tcccctgact gtaatcaacg ccataaatcg
    24061 tcgtgagttt gtccgccagc gccagcgata cggtacccgg tccgcagccc atatcgaata
    24121 aggtttgcgc gccttgcagg tcaatttttg aggtcagttg ttgcaggtag ctgtccgtcg
    24181 gcgaggcgca attttccgcc attttttccg cccgttgatc ccagtgatcc ggcgtttttt
    24241 ctgtgcgatg ggccagcctg agttgctgaa ggtaaaggtc tgcaaaatca atatcgtcaa
    24301 tgagcatgat ttttccttag tgcttaattg cagagaggtg atggtgaatc tggtcggcag
    24361 aaacccggta cagggcagcc agttttttcg ttgttaactg ttcggtcggt aagccttgtg
    24421 ttactcgtcc atctggctct acctggataa tgctgtcggc aatggcgttt gcatggagcg
    24481 gatgatgagt cgacattaac attgtcatgc cgttattttt gagctgcgcc agcgtgtcca
    24541 gaagttggat ctgatgaccg aaatcgaggc tagaggcagg ctcatccagt aataacaagc
    24601 gcggttgctg gacgagtgca cgggcgatta aagccagctg acgttcaccg ccgctaagcg
    24661 tattccagcg tcgtgtcgcc agatgcaaaa tgcccagctt ttccagttgc tccgttgctt
    24721 tcaggcgttc ttgcttaccg ggaacggaaa aagcgcctat ggtgggggcc tggcccatca
    24781 acaccatatc cagcacacta aaagcaaaaa tgccgtcgtg cgcctgcggc acccaggcaa
    24841 tagcctgcgc gcgctggcga tggctgagtg tctgcacagg gaggctatcc agtaagatct
    24901 cgccttttaa taacggtata acgccaagaa tggagcgcat gagtgtggtt ttgccgcaac
    24961 cattggctcc taacagacaa caaattgttc cttgcgacag ggtgaaggac acgtcgcgta
    25021 gcaccggatg gcgggagtaa cccagagacg catgacggac ggcaagcaaa gtcactggcg
    25081 acctccgcgt aacaacaaaa acaggaaaaa cggtgcgcca acaaaagcgg tcagaatgcc
    25141 aagtgggatt tctgttgttc caatgctgcg tgccagcgta tcggtaagca ggagcagaat
    25201 cgcgccagta cacattgcca tgggcaacag ctgctgatgg ttatgaccgg tcaacaagcg
    25261 ggcaatgtgc ggaacgacca ggccgaccca accaattatg cccgctattg ctaccgtgct
    25321 ggcggtgatg agtgtggcac agacaatcaa tccaaagcgc agacgtgtca cgttcagtcc
    25381 cagcgaacgg gcttcgtcgt cggaaagcgt gagtaaattc atgcgccagc ggagaaaaaa
    25441 caggggcaag gagcctatga gaatgatcgg tgcagcatag cagagatcgc gtaaggtcac
    25501 ggtggacagc ccccccagca accagaaggt aattgacggt aactgggtat aaggatcggc
    25561 cagcgttttt attagcgata tccccgctcc gcacaacgtt cctaacgcaa tgcctaccag
    25621 caccagggtt aagatcggat cgtggcgggt cacccgacgc gtaatcaacc agacgcccgc
    25681 gaccaccatc agcccgccgc aaaatgcgta cagctggatg taaacaatgg ataatcccca
    25741 caggattgcc gcgcaggcac caagtccggc accggcggag acgcctaaaa tatccggtga
    25801 cactaaggga ttacggaaca ttccctgata ggtggttccg gcccctgcca gcgccgcgcc
    25861 cagaaacagc gcagcaagaa tacgcggcac gcggatttgc cagaagacaa tttgttcctg
    25921 cacagtaaca ttttcttgac gaagaatgag cgccagcagt tgctgcatat cgagatgata
    25981 agccccactt acggcggcaa aagtgatact gatagctgcc agcagcaaaa tacatccgtt
    26041 tacggttctc attagctcgc caccaatttt tgatagtcgg cgtcacttaa tgggcaatgc
    26101 cagaacagtt gggcgtaatg ctgcatgtca cttttaaact ggcgattgat ggcgggatcc
    26161 agccaggcgt gaagtctgcg taatcccaga agacggttga ttcccggcgg ggcatccagc
    26221 cagccaaagg gcaggccact taaaaataag atgcgttgtt ccgcaaccgc tttcacgccc
    26281 tgccagagag gatcacgacg aataaaatct gcagtaacgg cctcctgaac cagaataata
    26341 tccggctgcc agcgcaggag attttccatg gaaacctgtg tcagaccgtg gcgatcggct
    26401 atttgcgcga cgttgtgcaa acctaataat tccgccgcct cggtatgcaa cgaaccctgt
    26461 aagcccgttt ccagccctcg aggaccgcgc gcagcataaa agctgagatt agcggcgggt
    26521 gaggtggcga atgcctgagc ttcaccaacg aagcggctgg cgagattggc ttgttctgcg
    26581 gcgcggtgct cttcgcctaa cgttttgcca agcgttgtta actgttctgc tgattgttcc
    26641 agtttcccgt taagcaataa ccagggtatt tgtgtctgtt cgctaacctg ccgtgcctgg
    26701 gagatccagg tttcatccgt attgccgcaa tcaacgacca aatcgggatg taacgccata
    26761 agcccttcca gcgagagtgt actggcgcgc ccggcgagtc gtcccagcct ggggaactgg
    26821 cgaatgtgct ctggcaatgg aattaatgcc tgacgggcaa aatcaaaaga ggaaaaaccg
    26881 accatttttt ctggcgcgac agccagcaat agtaaatcgg cgggcgcgcc tgcgctgacg
    26941 atgcgtgtaa tgttttgggc ttcgggaagc gtcagtgagg cccagaattt gactgattgt
    27001 gcaaaggacg gcagatgatg caggaacaat gtcgacgcaa gaatctgtgt aaacctccgt
    27061 ctggttagtg acataagtaa tatcctgata attaaaaccg ctatatactt acacatatag
    27121 cgaaaaagat catgtttgat gtcaaaattt gtctaaagag acggaatgat ttgcttgttg
    27181 cttaaataac actttgttcc acaagaaaat tgtcagggag aatggggagt gggggaaaat
    27241 ataaatagag gtgtaatgat taacaagttg ataaaataaa attaaaatct tctctaataa
    27301 aagaaaataa aggcatagac cgtagaaaga gtggtatttg gtatttcgtg gtgaagaaag
    27361 agcaatgcca attttatgca cgtaatggca ttgctcgata tacagtaaag ttaaggcgta
    27421 ggttccgcct cattttgttt taccggctgt gttgttgatt cactgagtgg acgcgtcgcc
    27481 ggaacattgt cgcacggttg ctctttcggg cagatcaaat ccagcatttt cagcgtcacg
    27541 ccattggtcc agccaaagcc atcctgtaat ggatattcgc caccgccgcc ccctgttccg
    27601 gtggtgctga catcatattt ttccaccagc tttttctccc ggtcataggt gtgctgaaca
    27661 ttggtcagga agtgccagct aatgtccatc gccacctctt tttgcccgta gttttgtaat
    27721 ccttctgtcg cgacccactg caacggtgcc cagccgtttg gcgcatccca ttgttgccca
    27781 cttttcaccg acgtggtgtt caggccgcct ggttgcagca gatgtgtttt cgtcgccgtc
    27841 gccattttgc tggcgcgatc tttcgctgcc gcattgacgt acagcgggaa cagggcggcc
    27901 gcggttaact gattgcgcac tttatgactt ttcaggtcgt aatcggcata ccagccttgt
    27961 tgatcgttcc acaggtattt ttcgatccct ttttgacggg cattcgccag cgtttcgtac
    28021 tggtttgcca tcgcgttatc tccggcagct ttgctggcgc gggcgaggat tttttccatt
    28081 ttaaacatca ggctgttcag atcgaccggt acgatgctgg tggtgcgtaa ggtatttaac
    28141 tgctgcggat tgtccatcca gcgcgagctg aaatcccagc cagacgcagc ggcagagcgc
    28201 aggtcgcggt aaatttcagt ggcaggtcga ttcggattgc ttttggcggt ggcaatatct
    28261 tccacccatg actctggtcg tggcgtatcg cgatcgtccc agtagcggtt gagaagggta
    28321 ccatcctgaa gtttgacaac gcgtttttcc tgttgtccgg cttgcaggtt ttcaacaccg
    28381 tccatccagt aagcatattc tttttgcatt tgcggcaggt attgcttcaa cgcggcatcg
    28441 ccttcatgct gcgccagtaa ctctaccatc agggcaaaga atggcggttg tgagcggctt
    28501 aaatagtaac tgcggttgcc gttgggaata tgcccgtaag tgtctatttc atgagcaaaa
    28561 ttggccacca tatccgcgac tttatcccag tgaccacttt cggcaagccc taacatggtg
    28621 aagtaactgt cccagtaata tacctcgcga aaacgtccgc ccggcacaac ataaggttcc
    28681 ggcagcggta acagagaatc ccatttttcg gtattttcgg tagaacgcgt taataccggc
    28741 caaagtccgt caatatgttc gcgtagtgat tgcccctctg gcggaacata tttctcgccc
    28801 tctttcggca gggtgaaatt gacgttaacg aaatggcgta gatcaaatcc gctctggttt
    28861 tgctgcatcc gataatcagc aaggatcatc agcggatcgc tgttcggcac ggcatcggca
    28921 aaggtttttt ggtccggaaa aagtttggcg ttttgcacat cattaaacag cggccctaat
    28981 aaaatatcag gcggctgtgg cgttaccggt gtttcttctg cctgcaccga tagcgcagcg
    29041 aaacacaaaa agatgcaggc tggaattaac gccattttta gcgggcgaga agctgcgggg
    29101 gatttcatca atcattctcc tttggcgaaa ccgaataaaa cggtattcaa gtatcagaaa
    29161 accttagttc aggatcgcac tagctgcatg atcgaaatct cattctgcga acggtcaggc
    29221 attttagaaa agggaattaa agggataaat aacgtcgaaa tagagtgatc aaataacgtc
    29281 tgtttgttag ccagccagcg gcgttgggtt cgcgattagc gcagaaaaaa tcatcatgat
    29341 ttggtaaaaa atataacagg tagttaaaaa tcatccgtgc agagtaaata gccggatggc
    29401 atcagaacga tgccatccga acggtggttt aaccctgacg gctgaaacgt tgcgttttaa
    29461 cgtccagcgt tagcgtttct tctggttgta tcgcatacag tttctcaccc tgctggcaaa
    29521 tccagccaat ccccagttca cgggcgatca gggcgctgtg ggatagcgga cttccggcgc
    29581 taaggcagat accttttaca accgccggat ccagttgcag tattgtggag ggatagatgt
    29641 tctccgccag tagaatagtt ggcgagttaa actgcgggag ttcttctttc gtttgggtca
    29701 ggtggaccag ggtgcgatgc agaagatcgt ccacatcaat atagcgagcc tgtagatatt
    29761 catcatccag ttgctggtat tgctggctaa gttctttaag aacttgctgc caggcatatt
    29821 ctgccgtgca atgttcatgc tgaaggagtt cgcttgccgc cgccagcagt tccggatcat
    29881 ctaacagtgt atggtgacca gaaaagattg cggcaatatc gtcaagcccg ctggcttctg
    29941 ctttcgctgt taacgtcatc agatctaata acgtgaagtc aatggcctgg cgtaatcgtt
    30001 cttgttcttc atccacggtc agggttgatt ttgcctgtac cgtacataaa accggttgat
    30061 aataaaaggc tttacccgaa acaggcggaa cgggacgcag aataggtgga gcaacttcct
    30121 ccgtttcacc aaagttatct tcagccagct gacggaaagc gatcagtgcc tcttcagctt
    30181 ctggcccttt cgcaatcagg cgcagcgtat cgttatagcg aacttgtagt aacgcaatct
    30241 ggttaatact ctccggtgtg acgcatttgc cgtttttctc cagcaacata tcggcattaa
    30301 atgtcgataa ggtataaacc agccgggagg ccggacgtac atgcaggccg ttacggtttt
    30361 ttatgaccac cgccagagaa cgggcttctt catcgtacga aggacatgtg tcagagattt
    30421 cagtgttgga ggagggtaaa cccagttgtt cacgtttggc ttccagcgca tgcatggcgt
    30481 caaagatcac tttgtcgatc tccgcccccg aggccgcgct aaccgttgct gccagtgtac
    30541 cttcgaccaa cggcgcagca cacaaacgta cttttgcggc gatctcggga gccagcaatt
    30601 ccagcgcagt ttcagcactc aataatgcgc ttcctatatc catcatgacc agcacatggt
    30661 cggtatcggc aacagattcg atggcctcca tcactttgac ggcatcggta ccaatgggat
    30721 tttgtggatc gtcaattccc gcggcaatgg cgattttaca attatcactc attaacatct
    30781 gacgggctaa ttcaccgacg ccttctccca gtcggctgct atgtgaaact atgaccaggt
    30841 ttaccatcac caattcctta ctcttttgcg gctaacgcca acatttgcat cataaacatc
    30901 accgaggtcg caccggggtc ctggtgacca atactgcgtt caccgagata actggcacgg
    30961 cctttgcgag cttgcatcgt aatcgtactt tgtgcagcgg attcggcgac gctactggca
    31021 gcttcgagcg ccgccggaat agagagattt tgctcgctgg actgacgtaa cgattccacc
    31081 accggcaccc acacatcaca catggtttta tcgccaggtt cggctttccc gcgactgatt
    31141 acgccgtccg cgccatcgcg gaacatctga taaagctctt ccagcgtcag gctttgccgt
    31201 gcctgggtcg cctgtgcggc gcggataaag aaggtaccga aaagcggacc actggcacca
    31261 ccgacgctgg aaagtagcgt cataccggta ttcttgagaa tgaaaccgat atctttatct
    31321 gcgatagcag ggagtttttc caccactttg ctaaagcctc ggttcatatt tagcccgtgg
    31381 tcagcgtcgc caatttcgcg atccagtccg gtaagatact cgctctcggt gctgaaaata
    31441 tcgccacaac gagtgagcca gttaacaatt tgagttctgc tcagtgacat tgctatctcc
    31501 ttatttaccc cagttaaggg ccggggtgtg gaccggggcg tcccagagtg ccagcgtttc
    31561 gtcatcaact ttcagtaagg tgattgagaa accggtcata tccagtgagg tgcagtacgc
    31621 gccaattaaa ttacgctcga tagtcaatcc cgcttgctgg caacgtgtgg tcaggcggtt
    31681 atagacgccg tacagctcag aaagcggagt tgcgccaaga ttgttaacca gcgcaatcac
    31741 ccgatcgcca gactggagcg gttgtttagt ttgtgattct tcctgccaac tgccttgttg
    31801 ataatcccag aaacgcaaag tgcgatggta tgagccattt tccagcaggg tgtcgaacat
    31861 ttcatcgacg gtttgatcaa gggaagagaa gggacggcgg tcaatacctg gctcaccatg
    31921 aatgccgacg ccaaactcca tctcattgtc cgccagggta aaagaaggtt tgcccgcggc
    31981 aggaacggta caggcaccga gagcgatacc tattgagtgg ccttgattat tcaacttacg
    32041 ccccagttcc gcacaggcgt ccagtgagtc gccacgctcg gccgctgcgc ctacgagttt
    32101 ttcaattaat acggtgttgg caacgccgcg tcgcccggcg gtataaagac tgtcttttac
    32161 cgcaacgtcg tcatcaatga ccacagtggt cacttttacg ccgctatcgt gcagtaactc
    32221 ggtcgctgtt tcaaagttaa gaatatcgcc ggtgtaattt ttgataatca acagtacacc
    32281 ttcgccgcca tcaatttgca tggcgcattc aaagatttta tcgggcgtcg gtgaggtgaa
    32341 aatttcgccc ggacaggccc ccgaaagcat cccctgaccg atataaccac aatgcatcgg
    32401 ctcgtgtccg ctgccgccac ccgacagcag ggcgacttta cctgcaacgg gggcatcaga
    32461 tcgggtgaca tacaccggat cctggtgcag tgtcagagat ggatgcgctt tcgctagtcc
    32521 tgccagttgt tcgtccagta cgtcttgcac atcattgatc aattttttca ttattttgct
    32581 ccagcaatta cggtagggca tggatgatgt tcaacgacac ggcgaccact gactgccgat
    32641 gaatccattg tgcatcaggc aagggaaaag aaaattagcc caaattatgt ttcatagtga
    32701 aacatattct ttaatgaatg ttccatattg aaacttttac gtgtattaat acttaaaatt
    32761 gcgagccgga acaccttttg tcataaggga tgcgggatat gagtagcgct tttaacaacg
    32821 atggtcggga catatctccc ttaattgcaa cctcctggga gcgatgcatt aagctgatga
    32881 aacgggatac atggaacgta ccacaccagg cccagggcgt gacatttgcg tctatttatc
    32941 ggcgtaagaa agcgatgctg acgctcgggc aggctgcgct ggaagatgcc tgggaatata
    33001 tggcaccgcg agcgtgtgcg ctgcttatcc tcgatgaaac cgcctgcatt ctcagccgta
    33061 atggcgatcc gcaaaccttg cagcaactaa gtgcactggg attcaatgac ggcacgtatt
    33121 gcgccgaggg aattattggt acttgtgcgc tatcgttagc ggctatctct ggtcaggccg
    33181 tgaaaacgat ggccgatcaa catttcaaac aggcactctg gaactgggcc ttttgtgcaa
    33241 cgccgttgtt tgacagcaag ggccgattga cgggaacaat agcgctggcg tgtccggttg
    33301 agcaaactac cgcagctgat ttgccgttga cgttggcaat cgcacgcgag gtcggaaatt
    33361 tactgctgac ggacagtttg ctcgctgaaa ctaaccgtca tttaaatcaa cttaatgccc
    33421 tgttagaaag tatggatgat ggcgtgatta gctgggacga gcagggtaat ttgcaattta
    33481 ttaatgccca ggcggcgcgg gtcttacgcc ttgacgcgac ggcaagtcag ggacgggcaa
    33541 tcactgaact cttaacgtta cccgccgtat tgcaacaagc aataaaacag gcacatccgc
    33601 tcaaacacgt agaagcaacc tttgaaagcc agcaccagtt tattgatgcg gtgataaccc
    33661 ttaaaccgat aatagaaacg cagggaacca gctttatttt gttgctccat cctgtggaac
    33721 agatgcggca gttaatgacc agtcaattag gaaaagtcag ccatactttc gctcatatgc
    33781 cacaagagga tccgcaaacc cgccgtttga ttcattttgg tcgccaggcg gcgcgcagta
    33841 gctttcctgt cctgctttgt ggagaagagg gcgtgggcaa ggcactgcta agtcaggcaa
    33901 ttcataatga aagtgagcgt gcagcagggc cttatatcgc cgtcaattgt gagttatatg
    33961 gtgatgcggc gctggcggaa gaatttattg gcggcgatcg cacggacaat gaaaatggtc
    34021 gtttgagtcg actggaactg gcgcacggcg gcacgctgtt tcttgaaaag attgaatatc
    34081 tggcggtgga gttacagtct gctttgcttc aggttatcaa gcagggtgtt atcacgcgac
    34141 tggatgcgcg gcgtttaata cccattgatg tcaaagtgat tgccacaacg accgcggacc
    34201 tggcaatgct ggtggaacaa aatcgtttta gtcgccagct gtattacgcg ctgcatgcat
    34261 ttgaaattac catcccgcct ctgcgtatgc ggcgtggcag cattccggcg ctggtgaata
    34321 acaaattaca cagtcttgaa aaacgcttct ctacgcggct gaaaattgat gacgatgccc
    34381 tcgctcgcct ggtttcctgt gcatggcctg gcaacgattt tgaactttac agcgtcatcg
    34441 agaatcttgc tctgagtagt gataacgggc gcattcgcgt cagtgattta cctgaacatc
    34501 tctttaccga gcaggcgaca gatgatgtca gcgccactcg cctttccacc agtctgtcat
    34561 ttgcggaagt tgaaaaagag gcaattatta acgcagccca ggtcacaggc ggtcgcattc
    34621 aggaaatgtc ggctttactt ggaatcggcc gcactacgct gtggcggaaa atgaagcaac
    34681 atggcattga tgcagggcag tttaagcgcc gtggatagcg ggagagtatg tactttcccg
    34741 ctatgaattg ctaattaata gcgaccatga ctgccgtgat ttggtccacc atttgaaggt
    34801 ggcattggct taccggaggg cgcaggacca cgagggccat cgctgtggcg gtgatgatgg
    34861 tggggaacac aagcacttaa caggctcgca aagacaattg ctaacaggct gaaggtaaat
    34921 gtttttttca tgacaaactc ttgaacggtt aatgcactgt gattgttatc acataaaagg
    34981 cacattgtta atattgccag gagtctgatt atgattttcg ataactcaga attgaaagaa
    35041 gccgcgtagc aggcgtaaaa aagccattat gtgcgactca cataatggct cattattaac
    35101 cgtcagggta ttacgtggaa tttagacgtt gaacaggaag ttcatcacat cgccatcttt
    35161 aacgatgtaa tctttacctt ctgcacgcat tttgcctgct tctttcgcgc cttgttcacc
    35221 tttgtaagtg atgaaatctt caaacgagat agtctgtgcg cggataaagc ctttttcgaa
    35281 atcggtatgg attttgccag ccgcttgcgg tgcagttgcg ccgacgggga tggtccatgc
    35341 gcgcacttct ttcaccccag cggtgaagta agtttgcagg ttcagcagtt tataaccggc
    35401 acggatcaca cggttcaggc ccggctcttc cagtcccagt tcctgcataa actcttcacg
    35461 ttcttcatcg tccagttcgg caatgtctgc ttcaacagct gcacaaaccg gaaccacaac
    35521 agaaccttct ttcgccgcga tttcacgcac ctggtcaaga tacgggttgt tttcaaaacc
    35581 gtcttcgttg acgttggcga tgtacatcgt cggtttcagc gtcaggaagc tcaggtaacg
    35641 aatagccgct ttttcttctg cgcttaaatc cagcgcgcgc agcatgcccg cgttttccag
    35701 ctgtggaagg catttttcca gtactgccag ttcggctttc gcgtctttat cgccgccttt
    35761 ggctttcttc tgtacgcggt gaatcgcgcg ctcgcaggtg tcgagatccg ccagtgccag
    35821 ttcggtattg ataacttcaa tatcgtccgc cgggttaact ttaccggaaa cgtgaatgat
    35881 attgtcattc tcaaagcagc gaacaacgtg gccgatcgct tcggtttcac gaatgttggt
    35941 caggaactgg ttacccagac cttcaccttt cgacgcgccc tttaccagac cggcgatatc
    36001 gacaaattcc atggtagtgg gaagcgtacg ctgcggtttt acgatttcag ccagttgatc
    36061 caggcgaggg tcaggcattg gtacgacgcc cgtgttcggc tcaatggtac agaatggaaa
    36121 gttggccgct tcaataccgg ctttggtcag cgcgttgaac agggtagatt tcccgacgtt
    36181 gggcaaaccg acgataccgc atttgaatcc catgatttaa atcaccttaa tatcttaata
    36241 atcaacctgt tattgctaac agattgcaga aatggaaata actttgccta ttatacacgg
    36301 cactcggcaa aaatgccgca gacaacgact tattgcgctt taaaggcgtg caatcggttc
    36361 gttgctttgg tcaagccatc tgtaaaccat atttcagtac aacgtgccgc ttcgtcgatg
    36421 gcttcatcaa ttaacttctg ttcactaaca ggcggtttgc ctaacacaaa accgacaact
    36481 ttatttttat cgcctggatg accaattccg atgcgtaaac ggtgaaagtt agggttatta
    36541 cccaatttac tgatgatgtc tttcagtcca ttgtgaccac catggccacc gcccaactta
    36601 aatttggcga cgccaggagg cagatccagt tcgtcgtggg ccaccagaat ctcgtccgga
    36661 ttaatgcgga aaaaactggc catcgccgca acggctttgc cgctgagatt cataaatgta
    36721 gtcgggacta acaggcggac atcttcgcct ccaagggtga ctcgcgaagt ataaccaaag
    36781 aatttagcct cttcgcgcag cggagcgcgc aatcgctctg ccagtaagtc aacgaaccag
    36841 gcaccagcat tatgtcgcgt tgcggcgtat tcagcgccgg ggttcgccag gccgacaatc
    36901 aatttaatcg tcacgttttt ttgtcctgag tgtgtacata actggcgcgt agtttactgt
    36961 ttgcggcccc gcttgacaaa aaactgcgta tcaaatgcag ataacgcaat aattgcctaa
    37021 gtggactatt agaaagtcaa ggtgttcagg cgtttatttg taaagttttg ttgaaataag
    37081 ggttgtaatt gtgatcacgc ccgcacataa cccacagggt gttgtctata ctttacacat
    37141 aaggaagagg ggtattccct gttacaaccc agaaagttcc ggaggtgaca tatgaaacgc
    37201 aaaaacgctt cgttactcgg taacgtgctc atggggttgg gtctggtggt aatggtggtc
    37261 ggcgtggggt attcaatcct caaccagtta ccacagttta atatgcccca gtatttcgca
    37321 catggtgcag tgctaagtat tttcgtcggt gccattctct ggctggcggg tgcccgtgtt
    37381 ggcgggcatg aacaggtgtg cgaccgttac tggtgggttc gccactatga caaacgttgc
    37441 cgccgtagcg ataatcgccg tcatagctaa caaataatgc ggtttgctga ccagtcagtt
    37501 tgcgctgact ggtcaatttc gtacttataa atccgccatc gccgcgcgac gattcgggaa
    37561 gaacgccagg cgtcccggga tcggttgaat gcctgcgcga gccatagtgc gcagtggctg
    37621 gaattccacg ttgcacacgc gcagttcaca tccttcgggt agacgcttca caaaacgctg
    37681 gaacgcatca agaccaccag catcaagtac cggaacggca tcccacttca gaatcacaat
    37741 ccgtttgcct tcaagacgtg actccaggtc cgtgaacaag ccttcagcgg cagcaaagaa
    37801 cagcgggcca ataacgcgca gaaccagaac atcgtctgga acgtctacga ctaccggtgc
    37861 caggcgagtc atacgtgcga tacgacgcat aaacagtagc gatgccagca cgatccccac
    37921 gctgatggca ataaccatat caaacagcac ggtcagcgac atgcacagca gcatgacgat
    37981 gatgtcatct ttcggcgcat gacgcagcaa gtcgaccact ttgtgcgctt cactcatgtt
    38041 ccacgccacc atcaacagca gggcagccat ggcggaaaga ggcagccagg agagcagcgg
    38101 tgccagtacc agcagggcaa gaataaccag aatagagtgg atcaccgccg agatagggga
    38161 agttgccccg gcacggacgt tagcggcaga acgcgcgatg gcagctgtag cggtaatacc
    38221 accaaagaac ggagcgatga tattccccag tccctgtcca accagttcac tgttcgcttt
    38281 gtgtttcgtc ccggtcatac catccagcac cacggcgcag agcagtgatt caattgcccc
    38341 taacatcgcc atcgaaaatg ctgccggaag cagagtgcga atggagtccc aggtgagggt
    38401 aaattcggaa ttgggcaggt cccacggcag taccagttgc ggcagcagtt gcggaatacc
    38461 gttaccctga gaaccatcgg ccagaacgta gtggaattgc gaaccgatgg tagcaacatg
    38521 tccgccgagc aggttaacaa tccccatcac cgcgcaacca gccagcaagg ccggaaggtg
    38581 accgggtaaa cgaatgccca gacgtggcca gaaaaccaga atgccaagcg tcacaatgcc
    38641 aatggcagca tcacccacat taatggtcgg caacgccata aataatgcgc cgactttttg
    38701 tagataatgt tcagggacat gggccatttg cagaccgaga aaatctttaa tctgcatggt
    38761 accgatggtg atcccgatac cggaagtaaa acctaaggta acggaaaccg gaatatattc
    38821 aatcaagcgt ccaaaacgtg ccagccccat caggatcaaa aagacacctg acatgagtgt
    38881 cgcgaccagc agtcctgcca gtccaaactg ttgcgaaacg ggatagagaa ttaccacaaa
    38941 tgccgcagtc ggaccggaaa cgctaaagcg tgacccaccc gtcagagcaa tgacaatccc
    39001 cgcaacagct gcggtatata aaccgtactg gggtgccaca ccactaccaa tagccaacgc
    39061 cattgccagc gggatagcaa taatcccgac ggttatcccg gcaatcaggt cacgggtaaa
    39121 ccgtgcggca gtatattttt ctttccagca agcgtcgatc agagcgcgga aaggcatcac
    39181 atgtgaggaa aatattttgt tcacaataat gtttcatccg tgagcgcatc atctgtcaac
    39241 taaatggcag gtgaaggagg cataggtcat acaaatggat attacagaca aaaaaacccg
    39301 ccgcagcggg tctttgagcc gggttcgatt agtgttcgaa catggcagag atcgattctt
    39361 cgttgctgat acgacgaatc gcttcggcca gcatacctga cagggtcaga gtacgcacgt
    39421 tcggcagtga tttgatttca tcgctcagcg gaatggtatc gcagacaacg acttcatcaa
    39481 ttacagagtt acgcaggttg ttcgccgcgt tgccagagaa gatcgggtga gtcgcgtacg
    39541 caaatacacg tttagcacca cgttctttca gagcttcagc agctttacac agcgtaccgc
    39601 cagtatcgat catatcatcg accagtacgc agtcacgacc tgcaacgtca ccgatgatat
    39661 gcatcacctg ggaaacgttc gcacgcggac gacgtttgtc gatgattgcc atatcggtat
    39721 cgttcagcag cttagcgata gcgcgggcac gcacaacgcc gccgatgtcc ggagaaacca
    39781 caattgggtt atccagattc agctgcagca tgtcttccag caggatcggg ctaccaaata
    39841 cgttatcaac cggaacgtcg aagaaaccct gaatctgttc agcgtgcaga tccactgtca
    39901 gcacacggtc aacaccgacg ctggagagga agtctgcaac cactttcgca gtgattggta
    39961 cacgagcgga acggacgcga cggtcctggc gcgcatagcc aaagtagggg ataacagcgg
    40021 tgatacgacc tgcggaagca cgacgcaggg catcaaccat aacgactaat tccatcaggt
    40081 tgtcgttagt aggggcacaa gtggactgga tgatgaaaat atcaccaccg cgtacatttt
    40141 cgttaatttg tacgctgact tcgccgtcgc taaagcgacc tacagcggcg tcgccgagtg
    40201 aagtgtacag gcggttggca atacgttgtg ctagttccgg ggtggcgtta ccagcaaaaa
    40261 gcttcatatc aggcacgaga agaacctcag gcatgcgtcc attggtggaa agaatctgcc
    40321 gaaaactgtg cgggccaggc atgattcttt ccaggcggtg tattaaagag cgcgatgcaa
    40381 cgtctggaac aaggtgacgt tgtcaccgaa actcagcttg cccggcttaa agcatggctc
    40441 tgtgcaatgg ggaaagatta acgcctttcg ccacaaagcc attgagccat tccggggctt
    40501 gctctagcac ctggcgggct tcagactctg tatcaaattc agcaaagaca caggcccctg
    40561 tcccagtcag gcgcgacggg gcgtattcta acagccagga aagcaccgca tcaacctcgc
    40621 gaaaacgttt tcttgcgata acctcgcaat cattgctgaa ttcacatttt agcaacgttt
    40681 ctattgacct ttttggcgta ttgcgcggga gttcaggatc tttaaaaatc accggtgtcg
    40741 gaatacttac accagggtgc gccaccaagt accacttctc tggcggatcc accggcgtta
    40801 gtatttcacc aacgccttcg gcaaacgcgg catgcccccg aacaaagaca ggaacatctg
    40861 cgcccagcgt cagccccatt tccgccagct catccatgct tagcccgcat tgccagagat
    40921 ggtttaatgc caccagcacc gtcgcggcat tggatgaacc accacccaga ccgccgccca
    40981 tcggcaaacg cttgtcaata ctgatttctg caccgcttcc cgtaggaaga cgcccgctgt
    41041 ctgccgctgt tttcatcagc aaccgtgctg cgcgaacgat caggttatct tcatgttcca
    41101 cgccttcaac gggtgttaac agacgaatat ccccatcgtc acgaagctca atgctgatgg
    41161 tgtcgccgta atccaggaac tgaaacagcg tttgcagcgt gtggtaaccg tccgcacgct
    41221 gaccggtaat gtataaaaac agattaagtt ttgccggaga gggccactgt gtccgcatta
    41281 tttcactatc cagttatcca tttttaactt gatgcgctga ccaccatcgg tgagttccat
    41341 attggatggc atcgcaggtt gagtttttgt gtcataacca ccgtaaacaa ctttccagtt
    41401 tttgccattc tggctgtagg tgatttcgct aaggcggtac tggtcgtcca gtttgtagtc
    41461 agtcgcatca cccggcaaac ctaaaatcca ctggcgtaag ctgttgagtg gaatgggcat
    41521 tccggtcaat ttgccaatca tctcttcggc gtcatcggcg gtataacgct ggcctttatt
    41581 gtcgactaac tgcacgttac ccggttgagc attcagctcc agttccgtgc tgcccaatgg
    41641 gttagtcagc agcagacggt agcgatcctg gccagtttgc tgccagaaga aacgggcgta
    41701 cactttttgt tggtcagaaa tataagcgaa cgcgccgcga gtttgatact gattaagatt
    41761 gcgcacgtct tgctgatgct gacgccattg tggcgaatcc gggcttttgc caggaccttt
    41821 gggcgtggta acggaacagg cagtgagcac aagagcagcc agcggtagta ggcggataag
    41881 acgaaaatcg ggcaggggca tagtgatgac aagtccttga gatacgttgc agttataacc
    41941 cttaatgcta gcgttaccgt ccgctatcgt ctatgttcaa gttgtcttaa ttgccagaat
    42001 ctaacggctt tcggcaatta ctccaaaagg gggcgctctc ttttattgat cttacgcatc
    42061 ctgtatgatg caagcagact aaccctatca acgttggtat tatttcccgc agacatgacc
    42121 cttttagcac tcggtatcaa ccataaaacg gcacctgtat cgctgcgaga acgtgtatcg
    42181 ttttcgccgg ataagctcga tcaggcgctt gacagcctgc ttgcgcagcc gatggtgcag
    42241 ggcggcgtgg tgctgtcgac gtgcaatcgc acggaacttt atcttagcgt tgaagagcag
    42301 gataacctgc aagaggcgtt aatccgctgg ctttgcgatt atcacaatct taatgaagaa
    42361 gacctgcgta aaagcctcta ctggcatcag gataacgacg cggttagcca tttaatgcgt
    42421 gttgccagcg gcctggattc actggttttg ggggagccgc agatcctcgg tcaggttaaa
    42481 aaagcgtttg ccgattcgca aaaaggccat atgaaggcca gcgaactgga acgcatgttt
    42541 cagaaatctt tctctgtagc gaaacgtgtt cgcactgaaa cagatatcgg tgccagcgct
    42601 gtttctgtcg cttttgcggc ttgtacgctg gcgcggcaga tctttgaatc gctctctacg
    42661 gtgacggtac tgctggtggg tgcgggcgaa accatcgagc tggtggcgcg tcatctgcgc
    42721 gaacacaaag tacagaagat gattatcgcc aaccgcactc gcgaacgtgc ccaaattctg
    42781 gcagatgaag tcggcgcgga agttattgcc ctgagtgata tcgacgaacg tctgcgcgaa
    42841 gccgatatca tcatcagttc caccgccagc ccgttaccga ttatcgggaa aggcatggtg
    42901 gagcgcgcat taaaaagccg tcgcaaccaa ccaatgctgt tggtggatat tgccgttccg
    42961 cgcgatgttg agccggaagt tggcaaactg gcgaatgctt atctttatag cgtggacgat
    43021 ctgcaaagca tcatttcgca caacctggcg cagcgtaaag ccgcagcggt tgaggcggaa
    43081 actattgtcg ctcaggaaac cagcgaattt atggcgtggc tgcgtgcaca aagcgccagc
    43141 gaaaccattc gcgagtatcg cagccaggca gagcacgttc gcgatgagtt aactgccaaa
    43201 gcgttagcgg cccttgagca gggcggtgac gctcaagcca ttatgcagga tctggcatgg
    43261 aaactgacta accgcctgat ccatgcgcca acgaaatcac ttcaacaggc cgcccgtgac
    43321 ggggataacg aacgcctgaa tattctgcgc gacggcctcg ggctggagta gcagtacatc
    43381 attttctttt tttacagggt gcatttacgc ctatgaagcc ttctatcgtt gccaaactgg
    43441 aagccctgca tgaacgccat gaagaagttc aggcgttgct gggtgacgcg caaactatcg
    43501 ccgaccagga acgttttcgc gcattatcac gcgaatatgc gcagttaagt gatgtttcgc
    43561 gctgttttac cgactggcaa caggttcagg aagatatcga aaccgcacag atgatgctcg
    43621 acgatcctga aatgcgtgag atggcgcagg atgaactgcg cgaagctaaa gaaaaaagcg
    43681 agcaactgga acagcaatta caggttctgt tactaccaaa agatcctgat gacgaacgta
    43741 acgccttcct cgaagtccgt gccggaaccg gcggcgacga agcggcgctc ttcgctggcg
    43801 atctgttccg tatgtacagc cgttacgccg aagcccgccg ctggcgtgta gaaatcatga
    43861 gcgccagcga gggtgaacat ggtggttata aagagatcat cgccaaaatt agcggtgatg
    43921 gtgtatatgg tcgtttgaaa ttcgaatctg gcggtcatcg cgtgcagcgt gtgcctgcta
    43981 cggaatcgca gggtcgaatt catacttccg cttgtaccgt tgcggtaatg ccagaactgc
    44041 ctgacgcaga actgccggac atcaacccag cagatttgcg cattgatact ttccgctcgt
    44101 caggggcggg tggtcagcac gttaacacca ccgattcggc aattcgtatt actcacttgc
    44161 cgaccgggat tgttgttgaa tgtcaggacg aacgttcaca acataaaaac aaagctaaag
    44221 cactttctgt actcggtgct cgcatccacg ctgcagaaat ggcaaaacgg caacaggccg
    44281 aagcgtctac ccgtcgtaac ctgctgggga gtggcgatcg cagcgaccgt aaccgtactt
    44341 acaacttccc acaggggcgc gttaccgatc accgcatcaa cctgacgctc taccgcctgg
    44401 atgaagtgat ggaaggtaag ctggatatgc tgattgaacc gattatccag gaacatcagg
    44461 ccgaccaact ggcggcgttg tccgagcagg aataatggaa tatcaacact ggttacgtga
    44521 agcaataagc caacttcagg cgagcgaaag cccgcggcgt gatgctgaaa tcctgctgga
    44581 gcatgttacc ggcagagggc gtacttttat tctcgccttt ggtgaaacgc agctgactga
    44641 cgaacaatgt cagcaacttg atgcgctact gacgcgtcgt cgcgatggtg aacccattgc
    44701 tcatttaacc ggggtgcgag aattctggtc gttgccgtta tttgtttcgc cagcgacctt
    44761 aattccgcgc ccggatacgg agtgtctggt ggagcaggca ctggcgcggt tgcctgaaca
    44821 accttgccgt attctcgatc tcgggacggg taccggggcg attgcgctgg cgctggccag
    44881 cgagcgtccg gactgcgaaa ttaccgctgt agatcgtatg ccagatgctg tcgccctggc
    44941 gcaacgtaat gcccagaatc tggcgatcaa aaacatccat attctgcaaa gcgactggtt
    45001 tagcgcgcta gccgggcagc agtttgcgat gattgtcagt aatccgccgt atattgacga
    45061 gcaggaccca catcttcaac aaggcgatgt ccgctttgag ccgctcactg cgctggttgc
    45121 ggcagacagt ggaatggcag acatcgtgca tatcatcgaa cagtcgcgta acgcgctggt
    45181 atccggcggc tttctgcttc tggaacatgg ttggcagcag ggcgaagcgg tgcgacaggc
    45241 atttatccac gcgggatatc atgacgtcga aacctgtcgg gactatggtg ataacgagcg
    45301 cgtaacgctc ggccgctatt atcaatgaca agtttttcta cactgcttag tgttcatctt
    45361 attagtatcg cgctttcagt tgggctatta accttacgtt tctggctacg ttatcagaag
    45421 catccacagg catttgcgcg ctggacgcgc attgtgccgc cggttgtcga tacgctgtta
    45481 ttgttaagcg gcattgcgtt gatggctaaa gcgcacatcc tgccattttc cgggcaggca
    45541 cagtggctga ctgaaaagct gtttggagtt atcatttata tcgttttggg ttttattgca
    45601 ctcgattatc gtcgtatgca cagtcagcag gcgcgcatta ttgccttccc gctggcgttg
    45661 gtggtgctgt acatcatcat taaactcgcc accacaaaag taccgttact ggggtaagtc
    45721 atgagatcgt tagctgattt cgaatttaat aaagcgccat tgtgcgaagg catgatcctg
    45781 gcttgcgaag caatccgccg cgattttccc tcgcaagatg tttacgacga actggagcgt
    45841 ctcgttagtc tggcgaagga agaaatcagc cagcttctgc ctttagaaga gcagctggaa
    45901 aaactgatcg cgctgtttta cggcgactgg ggatttaaag cctcacgcgg cgtttatcgt
    45961 ctttccgatg cattatggct ggaccaggtg ttaaagaatc gacagggcag tgcggtatca
    46021 ttaggtgccg ttttattatg ggtcgcgaat cgtcttgatt tgccgctgct gccggtgatt
    46081 ttccctacgc agctgatatt gcgcattgaa tgtccggatg gcgaaatttg gctgattaat
    46141 ccttttaacg gtgaatcgtt aagcgaacat atgctggacg tatggttaaa gggaaatatc
    46201 agcccgtcgg cggaactgtt ttatgaagac cttgatgaag ctgataacat tgaggtaatc
    46261 cgcaaattgc tggatacact caaagcttcg ttgatggaag agaatcagat ggagctggcg
    46321 ttacgcacca gcgaagcttt attacaattc aacccggaag atccctatga aattcgcgat
    46381 cgcgggttga tttatgcgca actggattgc gaacacgttg cgttgaacga tttaagttat
    46441 ttcgttgaac agtgtccgga agacccgatc agcgaaatga tccgtgcgca aataaataac
    46501 atcgcgcata aacatattgt gctgcattaa ttaatcgaca ttttactcaa gattaaggcg
    46561 atcctatgaa acaaaaagtg gttagcattg gcgacatcaa cgtagcaaat gacctgccgt
    46621 tcgtactgtt tggcggtatg aacgtgctgg aatctcgcga tctggcgatg cgcatttgcg
    46681 agcactacgt aaccgtgacc cagaaactgg gtatccctta cgtgttcaaa gcctcttttg
    46741 acaaagccaa ccgctcctcc atccactctt atcgtggacc gggcctggaa gaagggatga
    46801 aaatcttcca ggagttgaag cagacttttg gtgtgaaaat tatcaccgac gttcacgaac
    46861 caagtcaggc acagcccgtt gcggatgtcg tggatgtgat tcagttgccg gcgtttcttg
    46921 ctcgccagac tgacctggtg gaagccatgg cgaaaaccgg tgcggtaatt aacgtcaaga
    46981 aaccgcagtt tgttagcccg ggacagatgg gtaatatcgt tgataaattt aaagaaggcg
    47041 gcaacgaaaa agtgattctt tgcgatcgcg gtgctaactt cggctatgac aacctggttg
    47101 tcgatatgct gggcttcagc atcatgaaga aagtgtctgg taattcgccg gtgattttcg
    47161 acgtgaccca cgcactgcaa tgccgcgatc cgtttggcgc agcttccggc ggtcgtcgtg
    47221 ctcaggtggc tgagctggca cgtgccggta tggcggtagg tctggcgggg ctgtttattg
    47281 aagcgcatcc ggatccggaa catgcgaaat gtgatggtcc atccgcgctg ccgctggcta
    47341 aactggaacc gttcctcaag cagatgaaag cgattgatga cttggtgaaa ggtttcgaag
    47401 aactggatac cagcaagtaa tcttttttgc ctgaaaacta aagccttagc gttctgcgtt
    47461 aaggcttttt tcatgggggc cggatacaaa aagggccgca ggctgttacc cctgcggccg
    47521 gtttcgggcg catattgcca tcacggcagc ctgacgcccg ttttcgcctt acttccggtt
    47581 acgccaccag ccgacaatcg ctgcggtaat aattcccgcc aggatcggtg ctgccaggtc
    47641 gtgccagaaa atcacggtaa actgcgcgag cgtcatatag ccgccttgtt gtaatgacaa
    47701 cattttgcgg ctattcttga attgttctgg ttcaagatta gcccccgttc tgttgtcagg
    47761 ttatacctct caacgtgcgg gggttttctc tttccagcaa ccaatgccac cagggataaa
    47821 gcccccgcaa cattgcgcct caccggataa cgccggcttg gtgtggatac tacgactcaa
    47881 ttcatcttca cttcatccct gaaatgtttg caatgaagag tgcattccgg tttttcaaca
    47941 gctgttacag tcgtttcgtg caggttgtgg ttcaggctcg caattctgat taacaacctg
    48001 tccaggtagt atgaagaaat acaataagca ggaatttata ttatctcccc cgatttccgt
    48061 catcggagga gatataactg tcaggcaaat atcgtcatca aataggcggc aaacagtgcc
    48121 agatgcgctg cgccattgag cacgttagta cgtccggtgg agaaggagat atgacacagc
    48181 actaaagagg ccaccatcac caccatttct ggcgcaccaa gtgcaaactg caattcgtta
    48241 cccgtcataa aggcaattag cgtgacgaca ggtacggtaa gcgaaatggt tgctaacact
    48301 gaaccaaaga acagattcat cgcgcgctgg acctggttgt tcaacactgc ttttaacgca
    48361 cctaaacctt ccggcgacag aatcaacaga gccaccagga agccagtaaa ggcgacaggg
    48421 gcattcatgc tgtcgagcaa tgtctccagc gggctggcgt tcattttggt caccgcaata
    48481 acggcaatca gatggacaat caaccagata gcatgccaca ggctgctatg ggcagacggt
    48541 ttaccgtgat gcgggtcgtc gtcatcactg tcatcttcgt gctcgtagac aaacaaactt
    48601 tgatgcgttt tggtctggat cagcaaaaat acgccataca ttgccgcaga aattaatgct
    48661 accagtaacg cctgaccggt tgaaaaattc gccgcaggca gagccattgg aaataccagt
    48721 acgattatcg ccagggggaa cagcgcaatt aaatactgct tgataccaaa cagattcata
    48781 tattgggtgg caaacttgcg tccgcccaac aataatgaaa agccaaccag cccaccggta
    48841 acaatcataa tgattgaata gagcgtatca cgcattagcg ttggcgcggc gtcgccggtt
    48901 gccattaaag ctgaaatcaa actgacttca agaataacca ctgaaaggct aagaataagc
    48961 gaaccgtaag gctctcccag gcgatgggct aatacgtccg catgacggac aacactaaag
    49021 gcgctactta aaataccaat aagagcaaga agattgatgg caatgaccac tggtagtgtc
    49081 tggctgcttc cccacaggaa cagcactacc agcgccagaa ccgggaaaat aagcgaagtc
    49141 tccttgtggc gggtttttac cgcctcttga gcatttg
//
LOCUS       DAZKGF010000012        49039 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZKGF010000012 DAZKGF010000000
VERSION     DAZKGF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.012"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            305..2698
                     /gene="malP"
                     /locus_tag="ACHOQV_000709"
     CDS             305..2698
                     /gene="malP"
                     /locus_tag="ACHOQV_000709"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFP9629128.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELAEYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWTNDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPQVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2708..4792
                     /gene="malQ"
                     /locus_tag="ACHOQV_000710"
     CDS             2708..4792
                     /gene="malQ"
                     /locus_tag="ACHOQV_000710"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFP9629129.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTFTQEDQRVHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQTRDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QVAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGSETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLEAMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(4837..6153)
                     /gene="gntT"
                     /locus_tag="ACHOQV_000711"
     CDS             complement(4837..6153)
                     /gene="gntT"
                     /locus_tag="ACHOQV_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP9629130.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6514..7089)
                     /gene="nfuA"
                     /locus_tag="ACHOQV_000712"
     CDS             complement(6514..7089)
                     /gene="nfuA"
                     /locus_tag="ACHOQV_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFP9629131.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(7148..7831)
                     /gene="gntX"
                     /locus_tag="ACHOQV_000713"
     CDS             complement(7148..7831)
                     /gene="gntX"
                     /locus_tag="ACHOQV_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFP9629132.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALARLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7869..8639
                     /gene="bioH"
                     /locus_tag="ACHOQV_000714"
     CDS             7869..8639
                     /gene="bioH"
                     /locus_tag="ACHOQV_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFP9629133.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKMVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPAEFCRMLVALKQRV"
     gene            complement(8668..8787)
                     /locus_tag="ACHOQV_000715"
                     /pseudo
     CDS             complement(8668..8787)
                     /locus_tag="ACHOQV_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8859..9740)
                     /gene="rpnA"
                     /locus_tag="ACHOQV_000716"
     CDS             complement(8859..9740)
                     /gene="rpnA"
                     /locus_tag="ACHOQV_000716"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFP9629134.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAEHSPKHKESLMTIAERLRQEGFQKGIRAIAKTMLDAGVPLEDV
                     LRFTSLTVEDLAEINHQ"
     gene            complement(9943..10179)
                     /gene="feoC"
                     /locus_tag="ACHOQV_000717"
     CDS             complement(9943..10179)
                     /gene="feoC"
                     /locus_tag="ACHOQV_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFP9629135.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGNCKSCPEGKACLREWWALR"
     gene            complement(10179..12500)
                     /gene="feoB"
                     /locus_tag="ACHOQV_000718"
     CDS             complement(10179..12500)
                     /gene="feoB"
                     /locus_tag="ACHOQV_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFP9629136.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(12517..12744)
                     /gene="feoA"
                     /locus_tag="ACHOQV_000719"
     CDS             complement(12517..12744)
                     /gene="feoA"
                     /locus_tag="ACHOQV_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFP9629137.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(13182..15503)
                     /gene="yhgF"
                     /locus_tag="ACHOQV_000720"
     CDS             complement(13182..15503)
                     /gene="yhgF"
                     /locus_tag="ACHOQV_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP9629138.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVNGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(15600..16076)
                     /gene="greB"
                     /locus_tag="ACHOQV_000721"
     CDS             complement(15600..16076)
                     /gene="greB"
                     /locus_tag="ACHOQV_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFP9629139.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            16304..17023
                     /gene="ompR"
                     /locus_tag="ACHOQV_000722"
     CDS             16304..17023
                     /gene="ompR"
                     /locus_tag="ACHOQV_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFP9629140.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            17020..18372
                     /gene="envZ"
                     /locus_tag="ACHOQV_000723"
     CDS             17020..18372
                     /gene="envZ"
                     /locus_tag="ACHOQV_000723"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFP9629141.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGSSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(18404..18700)
                     /gene="nadS"
                     /locus_tag="ACHOQV_000724"
     CDS             complement(18404..18700)
                     /gene="nadS"
                     /locus_tag="ACHOQV_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP9629142.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(18759..19076)
                     /locus_tag="ACHOQV_000725"
     CDS             complement(18759..19076)
                     /locus_tag="ACHOQV_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9629143.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            complement(19191..20813)
                     /gene="pckA"
                     /locus_tag="ACHOQV_000726"
     CDS             complement(19191..20813)
                     /gene="pckA"
                     /locus_tag="ACHOQV_000726"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP9629144.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTRILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            21192..22916
                     /gene="yhgE"
                     /locus_tag="ACHOQV_000727"
     CDS             21192..22916
                     /gene="yhgE"
                     /locus_tag="ACHOQV_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFP9629145.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAEYMKDPKRRRGLLMAFDGVQRLQQQISEKA
                     LADNVFIASGSGKPDTIFWSAVMKNRYNAMICIEKDACVLVEQDLNSDGRAERILFAF
                     NDERYIIYGIDPAGKEWKEITMNSLPAVITKEKLLMAAEDGKLGTKPKVWRDLTVDGE
                     RLDLNLNE"
     gene            complement(23054..23932)
                     /gene="hslO"
                     /locus_tag="ACHOQV_000728"
     CDS             complement(23054..23932)
                     /gene="hslO"
                     /locus_tag="ACHOQV_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP9629146.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(23957..24358)
                     /gene="hslR"
                     /locus_tag="ACHOQV_000729"
     CDS             complement(23957..24358)
                     /gene="hslR"
                     /locus_tag="ACHOQV_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFP9629147.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(24369..25037)
                     /gene="yrfG"
                     /locus_tag="ACHOQV_000730"
     CDS             complement(24369..25037)
                     /gene="yrfG"
                     /locus_tag="ACHOQV_000730"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFP9629148.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(25102..27237)
                     /gene="igaA"
                     /locus_tag="ACHOQV_000731"
     CDS             complement(25102..27237)
                     /gene="igaA"
                     /locus_tag="ACHOQV_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFP9629149.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAHDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            27557..28117
                     /gene="nudE"
                     /locus_tag="ACHOQV_000732"
     CDS             27557..28117
                     /gene="nudE"
                     /locus_tag="ACHOQV_000732"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFP9629150.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRL"
     gene            complement(28285..30837)
                     /gene="mrcA"
                     /locus_tag="ACHOQV_000733"
     CDS             complement(28285..30837)
                     /gene="mrcA"
                     /locus_tag="ACHOQV_000733"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFP9629151.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            30957..31726
                     /gene="hofM"
                     /locus_tag="ACHOQV_000734"
                     /pseudo
     CDS             30957..31726
                     /gene="hofM"
                     /locus_tag="ACHOQV_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
     gene            31726..32265
                     /gene="hofN"
                     /locus_tag="ACHOQV_000735"
     CDS             31726..32265
                     /gene="hofN"
                     /locus_tag="ACHOQV_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFP9629152.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVDTVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQLQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDGHAL"
     gene            32249..32689
                     /gene="hofO"
                     /locus_tag="ACHOQV_000736"
     CDS             32249..32689
                     /gene="hofO"
                     /locus_tag="ACHOQV_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFP9629153.1"
                     /translation="MDMLFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            32679..33083
                     /gene="hofP"
                     /locus_tag="ACHOQV_000737"
     CDS             32679..33083
                     /gene="hofP"
                     /locus_tag="ACHOQV_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFP9629154.1"
                     /translation="MRVKRWLLAGITLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDAYTRRTGGKAAKSDADGG"
     gene            32995..34233
                     /gene="hofQ"
                     /locus_tag="ACHOQV_000738"
     CDS             32995..34233
                     /gene="hofQ"
                     /locus_tag="ACHOQV_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFP9629155.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            34634..35155
                     /gene="aroK"
                     /locus_tag="ACHOQV_000739"
     CDS             34634..35155
                     /gene="aroK"
                     /locus_tag="ACHOQV_000739"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFP9629156.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            35212..36300
                     /gene="aroB"
                     /locus_tag="ACHOQV_000740"
     CDS             35212..36300
                     /gene="aroB"
                     /locus_tag="ACHOQV_000740"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP9629157.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            36392..37678
                     /gene="damX"
                     /locus_tag="ACHOQV_000741"
     CDS             36392..37678
                     /gene="damX"
                     /locus_tag="ACHOQV_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFP9629158.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPELEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            37785..38621
                     /gene="dam"
                     /locus_tag="ACHOQV_000742"
     CDS             37785..38621
                     /gene="dam"
                     /locus_tag="ACHOQV_000742"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP9629159.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            38639..39316
                     /gene="rpe"
                     /locus_tag="ACHOQV_000743"
     CDS             38639..39316
                     /gene="rpe"
                     /locus_tag="ACHOQV_000743"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9629160.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            39309..40067
                     /gene="gph"
                     /locus_tag="ACHOQV_000744"
     CDS             39309..40067
                     /gene="gph"
                     /locus_tag="ACHOQV_000744"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFP9629161.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            40060..41064
                     /gene="trpS"
                     /locus_tag="ACHOQV_000745"
     CDS             40060..41064
                     /gene="trpS"
                     /locus_tag="ACHOQV_000745"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP9629162.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            41354..42259
                     /gene="yhfZ"
                     /locus_tag="ACHOQV_000746"
     CDS             41354..42259
                     /gene="yhfZ"
                     /locus_tag="ACHOQV_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFP9629163.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVNRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKQALLAHQQRVVSGEQEPSY"
     gene            42276..42638
                     /gene="yhfY"
                     /locus_tag="ACHOQV_000747"
     CDS             42276..42638
                     /gene="yhfY"
                     /locus_tag="ACHOQV_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9629164.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGMM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVKPCEE
                     GYLLANLYGLWMAANEEV"
     gene            42722..43885
                     /gene="yhfX"
                     /locus_tag="ACHOQV_000748"
     CDS             42722..43885
                     /gene="yhfX"
                     /locus_tag="ACHOQV_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFP9629165.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPLAHQ
                     GHLVQIPCHQVSDAVAQGTDVITVFTLDKAREVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVADIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLLSGIHRGEPEIVGRYDSLGNSLGA"
     gene            43885..45111
                     /gene="yhfW"
                     /locus_tag="ACHOQV_000749"
     CDS             43885..45111
                     /gene="yhfW"
                     /locus_tag="ACHOQV_000749"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9629166.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPMLEKMGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALLNADWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEVGVPTVLVGKVADIASNPYGVSWQNLVDSQRIMD
                     ITFNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLIEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMVATQLGVLTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            45108..45986
                     /gene="php"
                     /locus_tag="ACHOQV_000750"
     CDS             45108..45986
                     /gene="php"
                     /locus_tag="ACHOQV_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFP9629167.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     AQGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNIVKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            45997..46350
                     /gene="yhfU"
                     /locus_tag="ACHOQV_000751"
     CDS             45997..46350
                     /gene="yhfU"
                     /locus_tag="ACHOQV_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFP9629168.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            46362..47666
                     /gene="yhfT"
                     /locus_tag="ACHOQV_000752"
     CDS             46362..47666
                     /gene="yhfT"
                     /locus_tag="ACHOQV_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFP9629169.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            47678..48763
                     /gene="yhfS"
                     /locus_tag="ACHOQV_000753"
     CDS             47678..48763
                     /gene="yhfS"
                     /locus_tag="ACHOQV_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP9629170.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAPAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVSRIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HYAIRINPNRSGEETVLRILRESIASI"
     gene            complement(48870..49037)
                     /gene="yhfL"
                     /locus_tag="ACHOQV_000754"
     CDS             complement(48870..49037)
                     /gene="yhfL"
                     /locus_tag="ACHOQV_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFP9629171.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT        11544 a        12291 c        13430 g        11774 t
ORIGIN      
        1 cacactggat attttacttg tgaaactgtt tcactccttt acttaattat agagttacct
       61 tccgcttttt gaaaatacgc aacggccatt ttttgcactt tgatacagat tttctgcgct
      121 gtatggcatt gatttgctgc taatcctgtg gtttgcacta gctttaagtg gtcgagatca
      181 catttccttg ctcatccccg caactcctcc ctgcctaatc ccccgcagga tgaggaaggt
      241 caacatcgag cctggcaaac tagcgataac gttgtgttga aaatctaaga aaagtggaac
      301 tcctatgtca caacctattt ttaacgataa gcaatttcag gaagcgcttt cacgtcagtg
      361 gcagcgttat ggcttaaatt ctgcggctga aatgacccct cgtcagtggt ggttggcagt
      421 gagcgaagca ctggccgaaa tgctgcgtgc tcagccattt gccaaaccgg tggcgaatca
      481 gcggcatgtt aactacatct caatggagtt tttgattggt cgcctgacgg gcaacaatct
      541 gttgaatctc ggctggtatc aggatgtaca ggattcgttg aaggcttatg acatcaacct
      601 gactgacttg ctggaagaag agatcgaccc ggcgctgggt aacggtggtc tgggacgtct
      661 ggcggcgtgc ttcctcgact caatggcaac tgtcgggcag tcagctactg gttacggcct
      721 taattatcaa tatggcttgt tccgccagtc atttgtcgat ggcaaacagg ttgaagcgcc
      781 ggatgactgg catcgcagta actacccgtg gttccgccac aacgaagcac tggatgtgca
      841 ggtagggatt ggcggtaaag tgacgaaaga cggacgctgg gagccggagt ttaccattac
      901 cggtcaagcg tgggatctcc ccgttgtcgg ctatcgtaat ggcgtggcgc agccgctgcg
      961 tctgtggcag gcgacgcatg cgcatccgtt tgatctgact aaatttaacg atggtgattt
     1021 cctgcgtgcc gaacagcagg gcatcaacgc ggaaaaactg accaaagttc tctatccaaa
     1081 cgacaaccat actgccggta aaaagctgcg cctgatgcag caatacttcc agtgtgcctg
     1141 ctcggtagcg gatattttgc gtcgccatca tctggcgggg cgtaaactgc acgagctggc
     1201 ggagtacgaa gttattcagc tgaacgatac ccacccaact atcgcgattc cagaactgct
     1261 gcgcgtgctg atcgatgagc accagatgag ctgggatgat gcctgggcca tcaccagtaa
     1321 aactttcgct tacaccaacc ataccttgat gccagaagcg ctggaacgct gggatgtgaa
     1381 actggtgaaa ggcttactgc cgcgccacat gcagattatt aacgaaatta atacacgctt
     1441 taaaacgctg gtggaaaaaa cctggccggg cgatgaaaaa gtatgggcca aactggcggt
     1501 ggtacacgac aaacaagtgc atatggcgaa cctgtgtgtg gttggcggtt tcgcggtaaa
     1561 cggtgttgcg gccctgcact cggatctggt ggtgaaagat ctgttcccgg aatatcacca
     1621 gctatggccg aacaaattcc ataacgtcac caacggtatt accccacgtc gctggatcaa
     1681 acagtgcaac ccggcactgg cggctctgtt ggataaatca ctgaaaaaag agtggactaa
     1741 cgatctcgat caactgatca atctggaaaa attcgctgat gatgcgaaat tccgtcagca
     1801 atatcgcgag atcaagcagg cgaataaagt ccgtctggca gagtttgtga aagttcgtac
     1861 cggtattgag atcaatccac aggcgatttt cgatattcag atcaaacgtc tgcatgagta
     1921 caaacgccag cacctgaatc tgctgcatat tctggcgttg tacaaagaaa ttcgtgaaaa
     1981 cccgcaggct gatcgcgtac cgcgcgtctt cctctttggc gcgaaagcgg caccgggcta
     2041 ctacctggcg aaaaacatta tctttgcgat caacaaagtg gctgacgtga tcaacaacga
     2101 tccgcaggtt ggcgacaaat tgaaggtggt gttcctgccg gattattgcg tttcggcggc
     2161 ggaaaaactg atcccggcgg cggatatctc cgaacaaatt tcgacggcag gtaaagaagc
     2221 ttccggtacc ggcaatatga aactggcgct caatggtgcg cttactgtcg gtacgctgga
     2281 tggtgcgaac gttgaaatcg ccgagaaagt tggtgaagaa aatatcttta tttttggtca
     2341 taccgtggaa caagtgaagg caattctggc caaaggctac gacccggtga aatggcggaa
     2401 gaaagataag gtgctggacg cagtattgaa agagctggaa agcggcaaat acagcgacgg
     2461 cgataagcat gccttcgatc agatgctgca cagtatcggc aaacagggcg gcgatccgta
     2521 tctggtgatg gcggatttcg cagcctatgt agaggcacaa aagcaggtgg atgtgctgta
     2581 ccgcgaccag gaggcctgga ctcgcgcggc gatcctcaat accgcccgct gcggtatgtt
     2641 tagctcggat cgctctattc gcgattatca ggctcgtatc tggcaggcaa aacgctaagg
     2701 aagctcgatg gaaagcaaac gtctggataa tgccgcgctg gcggcgggga ttagccccaa
     2761 ttacatcaat gcccacggta aaccgcagtc gattagcgcc gaaaccaaac ggcgtttgct
     2821 tgacgcgatg catcaacgta ccgccacgaa agtggcggta acgccagtcc cgaatgtcat
     2881 ggtttatacc agcggcaaaa aaatgccgat ggtggtggag ggcagcggcg aatatagctg
     2941 gctgctgacc accgaagaag gaacgcagta caaaggccat gtaacggggg gcaaagcgtt
     3001 caatctaccg acgaagctgc cggaaggtta tcacacgctg acgtttaccc aggaagacca
     3061 gcgcgtgcat tgcagggtga ttgtcgcccc gaaacgctgt tacgaaccgc aggcgttgct
     3121 gaataaacaa aagctgtggg gtgcctgcgt tcagctttat acgctgcgat cggaaaaaaa
     3181 ctggggtatt ggggattttg gcgatctcaa agcgatgctg gtggatgtgg caaaacgtgg
     3241 cgggtcgttc atcggtctga acccgattca tgcgctctat ccggcaaatc cggaaagtgc
     3301 cagcccatac agcccgtctt ctcgccgttg gctgaatgtg atttatatcg acgttaacgc
     3361 cgtcgaagat ttccatctta gcgaagaggc tcaggcctgg tggaagttgc cgaccacgca
     3421 acagacgctg caacagacgc gggatgccga ctgggtcgat tactccacgg ttaccgccct
     3481 aaaaatgaca gcattgcgca tggcgtggaa aggtttcgcg caacgtgatg atgaacaggt
     3541 ggccgcgttt cgtcagtttg ttgccgagca aggcgatagc ctgttctggc aggcggcgtt
     3601 tgacgcgcta catgcccagc aagtgaaaga ggacgaaatg cgctggggct ggcctgcatg
     3661 gccagagatg tatcagaacg tggattcacc agaagtgcgc cagttctgcg aagaacatcg
     3721 tgatgacgtc gatttttatc tctggttgca gtggctggct tacagccagt ttgccgcctg
     3781 ctgggagata agccagggct atgaaatgcc gattggcttg tatcgtgatc tggcggttgg
     3841 tgtagcagag ggtggatcag aaacctggtg cgaccgggag ttgtattgcc tgaaagcgtc
     3901 ggttggagca ccgccggata tcctcggccc gttggggcag aactggggat tgccgccaat
     3961 ggacccgcat attatcaccg cgcgtgccta tgaaccgttt atcgagctgt tgcgtgccaa
     4021 tatgcaaaac tgcggcgcat tacgaattga tcacgtgatg tcgatgctgc gtttgtggtg
     4081 gataccgtat ggcgagacgg cagatcaggg cgcgtatgtt cactatccgg tggatgatct
     4141 gctctcgatt ctggcactcg aaagtaaacg tcatcgctgt atggtgattg gtgaagatct
     4201 cggtaccgta ccggtagaga ttgtcggtaa gctgcgcagc agcggtgtgt actcctacaa
     4261 agtgctctat ttcgaaaacg accacgagaa gacattccgc gcaccgaaag cgtacccaga
     4321 gcagtcgatg gcggttgcgg cgacacacga cctgcctacg ctacgcggct actgggagag
     4381 cggggattta acgctgggca aaacgctggg gctgtatccg gatgaagtgg tactgcgcgg
     4441 tctgtatcag gatcgcgaac tggcgaagca aggactactg gatgctctgc ataaatatgg
     4501 ttgcctgcca aaacgtgccg ggcataaggc atcgttgatg tcgatgacac cgacgctgaa
     4561 tcgtggctta cagcgctaca tcgccgatag caacagcgcc ctgttaggac tacagccaga
     4621 agactggctg gatatggcgg acccggtaaa tattcccggc accagttacc agtataaaaa
     4681 ctggcggcgc aagctctccg caacgcttga ggcgatgttt gccgatgatg gtgtgaacaa
     4741 gttgctgaag gatttggaca gacggcgcag agcggcagcg aagaagaagt agagtgcggt
     4801 tgatgccgga tgcgatgcat ccggcagtca gtgttatcaa atcaccatat tcagcagcag
     4861 acagcctacc agaccgcaca ccgagataat ggtttccagc atcgaccagg atttgatggt
     4921 ctcgccgata gtcaggttaa agtactcttt gaacagccag aagcccggat cgttcacgtg
     4981 agagaaaatc acactaccgg aaccaaccgc aataaccatc agctccgggc taacacccgt
     5041 cgttgcaatc agcggtgccg cgataccacc cgcagtgatt gccgcaacgg ttgcggaacc
     5101 cagcgcgata cgcagtacgg cagcaatcga ccaggccatc agcagcggag aaatgttggt
     5161 ttcgtgcatc atggaagcaa tgtatttgtc cacgccgctg tctaccagca cctgcttgaa
     5221 cgcaccgcca ccaccgataa tcaacagcat catcgcaatg attttgatgg aagaaaccag
     5281 cgtgtcgtta atctgatcca ttgaacgacc acggttcaga ccaaaggtga acatcgcaat
     5341 cagtacggca atcagcgtag ccattaccgg gtcaccgagg aactccgcta ccggcaggaa
     5401 agcgtgacct ttcggcagga tcatttcggc aatcgcacgc atcgccatca gcacaaccgg
     5461 gaccagagag gtccagacgc tgacgccaaa acttggcatc tcttcttcgc tgaaggtttt
     5521 cgcgctgtac agaccttccg ggatcggctt atctatacct ttcagcacgc gagcgtaaac
     5581 cggaccggcg agaatcacgg tcgggattgc cagaatagta ccgtacagca gggttttacc
     5641 catatcggca ttgaaaatgg tggcaatcgc agtcgggccc ggatgcggtg gcaggaagcc
     5701 gtgggtcaca gacagtgcag ccgccattgg tacaccaaca tacagcagtg gaatattcgc
     5761 ggaagccgcg atggtaaaca ccagcggcag catcagcaca aagcccactt catagaacag
     5821 ggcaaaacca acggtaaaac cggtcagtac caccgcccac tggatgtgtt ttttaccaaa
     5881 tttagcaatc agcgtggtgg cgatacgttg tgcgccaccg cagtctgcca gcattttgcc
     5941 cagcattgcg ccaaaaccca tgatcagggc aaggctaccg agcgttccgc cgacacctgc
     6001 tttgatggag ccaataactt tatccagcgg cattccttgc attaatccaa cagcaagcgc
     6061 cacgaggacg agagcgatga agccgttcat tttgaaacgg atcatcagga gcaacaacaa
     6121 gattacaccg atagcaacaa tgactaatgg catgatttac ctggcctttc atttgttatg
     6181 ggtaacgtca attttctgac gacaaactct aattatccca atcgggaaca gagatattgc
     6241 ggcaccacga ctgataccca ctaaaactaa ttattgtagt cagatgtcag gagtatgttt
     6301 ggtacccacg tgaatgatac gggtaacatc cggcgtttga gaatcaccag agcggggtaa
     6361 atttaaatta tgagaggttg gtcatattat cgcggggaaa cgaagggagg ggtttgccaa
     6421 agcaatgctg cgccaacgtc tggcacatgt tcaacgtagg cccgaaatga cgctttagcg
     6481 tcgcatcggg caatctacaa aagaggggat aacttagtag taggagtgct cgccgcgctg
     6541 gtgttcggtg agatcgcgca cacctttcag ctccgggaat tcgttcagca gctgcttctc
     6601 gatcccttct ttcagcgtca catctaccat agaacaaccg ttacagccgc cgccaaattg
     6661 cagaatggcg taaccgtctt cggtgatttc catcagcgaa acgcgaccac cgtgaccagc
     6721 aagctgtggg ttgatctgcg actgcagcat atactccacg cgctccatca gcggtgcatc
     6781 gtctgcgact ttacgcattt tggcgttagg cgctttcagc gttaactggg aacccaactg
     6841 gtctgtaaca aagtcgatct ctgcatcttc caggtaagga gcgcttaact catcaacata
     6901 cgcggtcagc aggtcaaatt tcagggcagt gtcggtggct tctacagcgt ccggcggaca
     6961 ataagaaacg ccacattcag cgttaggcgt gccagggtta atcacaaata cgcggatttg
     7021 tgtcccttct tcctgatttg ccagcagttt ggcaaagtgc gcttgtgcag catcggaaat
     7081 acggatcata gtaatggcct aatagttgac tattttagtt ggttataata cgcccatcat
     7141 cgaggctcta caaggttcga caaaggcacc agacctggac agtcgccgca ccattgcgta
     7201 aaagcaactg cgcaatctct gcgacggtac ttccggtggt gacgacatca tccacaatca
     7261 ccatatggcg accttgcaca ggcaattcaa ggcgaaaggc atttttcagg ttgcgcttgc
     7321 gcagccgggc actgagaaaa tgctgggtcg cagtggcccg tgttcgtgtg acggcttcgc
     7381 tatcccatcg gcagtgcaac cagcgtgata acggctgaca cagcaaatcg ctctgattaa
     7441 atccccgacg ccagtgacgc cgctgccata acggaacgct gataatgcga tccggcaatt
     7501 gcaacccggt ggtgcgacga gcgtgtaaga cttccaatag taacagacgt gccagggcgc
     7561 tggcgatttc gctgcgccgg gaaaacttaa gctggtggat aagcggactt aacggcggcg
     7621 catagtcggc aaccgtgacc agtctttgcc agggcggcgg tttttgcagg cagcgaccgc
     7681 agggaagatg tgagtgtgtg gcgggtaatc cacattgggg gcataacgtt ttatctgtac
     7741 gggcggcgcg tgagcatacc gaacaaatcc cccaatgacc taacgccagc ggcattcggc
     7801 atagccagca taatcccggt actgttagca tatgttcatc cttgtaagtc aaaagagaac
     7861 aatagcggat gaataacatc tggtggcaga ccaaaggtca ggggaatgtt catcttgtgc
     7921 tgctgcacgg atggggactg aatgccgaag tgtggcgttg cattgacgag gaactgagct
     7981 cgcattttac gctgcacctt gttgatctgc ccggcttcgg gcgtagccgg ggatttggtg
     8041 cgatgtcact tgctgatatg gccgaagccg tgctgcgaca ggcacctgat aaagccattt
     8101 ggttaggctg gagtctgggc gggctggtgg caagccagat tgcgttaacc catcccgagc
     8161 gtgttcaggc gctggtcacc gtggcgtcgt caccttgttt tagtgctcgt gacgagtggc
     8221 cggggataaa accggacgtg ctggcgggat ttcagcagca actcagtgat gattttcagc
     8281 gtacggtgga gcggttcctg gcgttacaaa ccatgggtac tgaaacggcg cgccaggatg
     8341 cgcgggcgct gaagaaaacc gttctggcgt taccgatgcc ggaggttgac gtgcttaatg
     8401 gcgggctgga aatcctgaaa atggtcgatc tccgtcagcc gctgcaaaac gtgtccatgc
     8461 cgtttttgcg attgtatggc tatctcgacg gtctggtgcc gcgcaaagtg gtgccgatgc
     8521 tggataaact ttggcctcac agcgaatcat acatcttcgc caaagcggcc catgcgccgt
     8581 ttatttcgca tccggccgag ttttgtcgca tgctggtggc gttgaagcag agggtgtagg
     8641 tggcttttga aatggcgaga cagaacttta ctggctcgcc gcagccaact cttcttctga
     8701 caccccggta aagcgcatga tgtctgcaag agggactccg gattacagca ttcttttggc
     8761 gatatccagg gcttccaggc gtcgacccaa taaataacca atcaggtaac taataagata
     8821 ttctctttct gcatctgtca ttaaaaccat cctttacgtt attgatgatt tatttcagca
     8881 aggtcttcaa ccgtcaggct ggtaaatcgc agaacgtcct caagagggac tccggcatcc
     8941 agcatggttt tggcgatagc tcgtatacct ttctgaaacc cctcttgccg caatctttcc
     9001 gcaatagtca ttaagctctc cttgtgtttc ggtgaatgtt cggcaacgcc gtcgataaaa
     9061 tcgttaaaac gtaccgcatc gccggtttgc aagatgtaat taaacagccc tttgatttgt
     9121 ctgtcattag cgtatccact acttaataag caggccattt gctctaccag ccccatcaga
     9181 tcgcgttgac gaatatgttt ttgaattaac tccagcagcg ccatacgtcg gtgctgcatg
     9241 atttcatcat caggcatgac ggtgacatca atcagcggaa atgcggaggc ataaagctgc
     9301 cttgccagtt tgggatcggc gaaacaatca agccaacaca gcgaataggg ataggggctt
     9361 tcaataccgt ggtaaaacaa caatggcacc accatcggca atgttttgta tccggcatcc
     9421 aggtgatttt gcattgcggc aatggcgtaa cgcatcatgc gaaatgcgat cagtttgttt
     9481 gaggtgcttt gatgttcaat cagacaatag atatatcctg gcccttgttc cgttttcacc
     9541 gaccacagca cgtcggaata gctttcacgc agatcgtcat caataaagct gctcgactcc
     9601 agcttgaggg ttttcatatc acagagcgcg tgaataggtg ccggtaaatg aaacgcaaga
     9661 aaatcgcgcg ccgtctccgg ttggcgtaaa aagagtttga acagcgcatc gtgtggggtg
     9721 gaactctgct ttttgctcat ggcgttcctt cacctgttgt ttgaatgccg cgactctacg
     9781 caccactcca cacctgtgca ttagcgagtt ttcatccctg cgagacgctt tcagaggaat
     9841 ttacgtcgcg ctgcaagggt tgcattttta tggaaagccg cacgcaaaaa agcccggatg
     9901 caaacgcata ccgggctttt caaaacaggt gatggagtat ggttaacgca gcgcccacca
     9961 ctcgcgcagg caggctttgc cttccgggca gcttttacaa ttgccagaga ggcagccgtc
    10021 aggttcttcc tgaatccgca cggctttgcc catgctttcc agctgttgca gcatggcgtt
    10081 aatcattggc tgcggggtgt tcaatgtctg gcttatctgg gccgcttcca tacggccccg
    10141 taacgccagc aaatcacgca cctgaataag tgaagccatt aatggcaatc accggtggtg
    10201 ctggctgcgc agcaactgct taccgacttg cgcgttgcca gcagttcgat atccacccgg
    10261 ctacgcgcgc gacgcagcaa accgataacc acgatgttaa acaggataac cgccagaatg
    10321 cacaccaggc tgtaagttgg gtgctggctg tagctggcga cctgatagaa caatgttgcc
    10381 agtgagtaag cgatattcag cccccacagg atggagaagc ccatccagcc acggcttgat
    10441 tcacgggcga tagcccccat caccgagatg cacggtacat acagcaggac gaaaatcagg
    10501 tagctgtatg cggcagctgc gctaccgaat ttctgatcca tcacgcccat cgccccggtc
    10561 cccatttcgc cgtcgccttt gctcgcttca atgggattca tcagtacgct aaggctgaag
    10621 gtgtctttca ggctctgcca ggtttcatct accgcgctga acagctcttc accgaggtta
    10681 aattctgccg gattgaactc ttcgtcctga atattttctg cggtgtagag ggtgttgagc
    10741 gtgcccacca ccacttcttt cgccattgcg cctgtaaaca ggccaaccgt tgcctgccag
    10801 ttatcttcat gcacgccaat cggcttgaag actggggtga tcacccggct gacggaagcc
    10861 agcgccgagt cgttgatgtt atcgacgatt tttccgctca gcgagaagct gttgaaggcg
    10921 ctcaggaaaa tgctgacgat gataatcact ttaccagcac gcagaacgaa gcctttcaga
    10981 cgctgccagg tctggataat caggctttta acgtgtggta catggtagac cggcagctcc
    11041 atgacaaacg gcgtcgcttc accgcgcatg atggtgtact tgagcatcag gcccgtcagc
    11101 accgccatta cgatacccag catatacagc gagaagaccg ccagcgcacc gttctgcccg
    11161 aagaaggcag ccgcgaatac tgcgaagata gccagacgtg cgccgcagga cataaacggt
    11221 gccatcatga tggtcatcag gcgttcacgc ggtgcatcaa gcgtacgtgc gcccattacc
    11281 gatggtacgt tacaaccgaa gccgacgatc agcggtacaa aggatttccc cggcaagccc
    11341 agcgcctgca tcaggcggtc catcacaaac gccgcacgcg ccatatagcc ggagtcctca
    11401 aggaaggaga ggaacaggta catcatgccg atctgcggca ccagtggcag cacggtgtta
    11461 ataccgccac ccaggccctg ggcgaggaag atagtcagcc agtccgggaa gtggagcgtg
    11521 tagccaatcc actgaatacc atgcacaaac agcgccacgg aaccgacatc aaacagcggc
    11581 tgtaacgccc cgccgatgtt gatagccagc aggaacatca ggtacatcac aaagaggaaa
    11641 atcggcagac cgaggaaacg gttgagcacg attttatcta ccgcagtggt gaaacggctg
    11701 ggttctgccg tcagggtgtt gcttaccaca tcacagatgg cagcaatgca ctggtaacgc
    11761 gcatcggcaa tgtgcagcgc cggatcgtcc atctcattac gcagacgggc gagggcggca
    11821 tccagatgct gcgacgcttc accggcgtag gcgcggctgt agatatcgcc ttccagcatt
    11881 tgcaggccca gccagcgacg ttgtttcagc gggatgtcgg aaggcatcac ttttgccagt
    11941 gaatctgctt cgttgagtag cggctgtgcg taatgcacca gttccacatt ctcgttagct
    12001 ttatagcgat caatcgccag cttgagcgct tcaataccgc gaccacgggt tgaaaccagc
    12061 gggatcaccg ggcagccaag acgcgccgac agagcatcaa tttcaatacg aatattttgc
    12121 ttctcggcaa tgtcgagcat gttcagtgcc acgatgcagg gaatgccgag ttccaacagt
    12181 tgcagcgtca ggtacaggtt acgctcaagg ttagacgcat ccaccacgtt aatcagcagg
    12241 tctgcgtcgc cactcaaaat gtagtgacag gcgatttgct catcgagcga ggtctgcgat
    12301 gagatggtgg tcagagaata ggtgccgggc aggtccacca gcgtgacctg atgatcggtg
    12361 gtggagaatt gcccttcttt acgttcgacg gtaacgccag cccagttacc tacacgctga
    12421 cgtgagccag tgagctggtt aaataacgtt gtcttgccag aatttggatt accaattaag
    12481 ccaatggtta attttttcat tgttgttatc accgtattaa caggaaaccg cttccacttc
    12541 taataaggcc agatcttttt ttcgtaatac caggctcaca cgacgggttt cgatatgaat
    12601 ggggtcgccg agtggagcga cgcgcaccac attaaaagag gagccaggta acatgccaag
    12661 agaaagcagt ttttggcgat atgccgggct gatttcacgg gaaaagccag taattttcca
    12721 cgcagtatct ggagtgtatt gcataggtgc ctacttgttt ctcattaact ggataactac
    12781 ctcatggggc gtttgacagc gacatgagac cagatgattt gccaacgaag gagagattaa
    12841 ataatcaaca gtacaatgat aatgagaatg gtttctatca gcaatactta aaatgtgtgt
    12901 gaatgttggt ttgaataata atctgactca atgttgatat ggctcaaggt tatatcgcag
    12961 cgaaaatata attcgcatga cgaattatta gggctggtta atgtttaatt aaggtttcag
    13021 ttgttaatga agtgataaat attaatggga tatattatga gtttgcttgt ggaataaaaa
    13081 ggccggagat attctccggc acttttatgt caggcaaggc gttcgttgcc agatgtggcg
    13141 tgaacgccta atcgcgcagg tttaggggcc agtccgggcg tttaacgttt tttgcccatt
    13201 gccgccgcca gcgcatccat catcgcgcta ttaccggcag gctgcgcttc acgcccacgc
    13261 ggtttggctg ccgggcggtt gttttgcggg cgatcgttac cgccaccgcg acgggcgttg
    13321 gtttcgccag gctgctcgtc cagacgcatg gtcagggcga tacgtttacg ctgaagatcc
    13381 acttccagca ctttcacctt cacaatgtcg cccgctttca ccacggtatg cggatcttcc
    13441 acaaacttgt tcgacaatga agagatgtga accaggccgt cctgatgcac gccaatatcg
    13501 acaaacgcgc caaagttggt gacgttggtc accgcacctt cgaggatcat acccggttgc
    13561 aggtcgttca ttgtctcgac gccatcggca aactgagcgg ttttaaattc cggacgcgga
    13621 tcgcgacccg gtttttccag ctctttgatg atgtcggtta ccgtcggcac accgaatttt
    13681 tcatcagtaa agtcagacgc tttcaggtta cgcagctcgc tgctgttacc catcagatct
    13741 ttcagtgcct gctgtgttgc tgccagaatg cgttccacca ccggataggc ttccgggtgg
    13801 acggtagacg cgtccagcgg gttatcacca tggttaatgc gcaagaagcc cgcgcactgc
    13861 tcgaaggctt tcggccccag acggctcact tttaacagtt gctgacggtt ctggaactgg
    13921 ccgttctcat cgcgccaggc cacgatgttt tgcgccatca tgcgcgtcag gcccgccacg
    13981 cgggttaaca gaggaacaga agcggtgttg agatcgacgc caacggcgtt tacgcagtct
    14041 tctactactg catccagttt gcgtgccagc tgcgtctggc tgacgtcatg ctgatactga
    14101 cctacgccga tagatttcgg atcgattttc accagctccg ccagcggatc ctgcaaacga
    14161 cgggcgatag acaccgcgcc acgcagcgaa acgtcgagat ccgggaattc ctgtgctgcc
    14221 agctcggaag ccgagtaaac cgacgcgcca gcttcgctga cgatcacttt ctgcgcggtc
    14281 actttcggga actgcttctg cacgtcgaga tagaaacgtt cggtttcgcg agaagctgta
    14341 ccgttgccga tcgccaccag ttcaacgtta tgcttttcac acagcgcagc aacggtcatc
    14401 gctgcttttg cggcctgccc ggtatgcgga taaatggtat cggtcgccac cagtttacct
    14461 gtcgcatcga ctaccgccac ttttaccccg gtacgcagac ccggatcgag gcccatcgtt
    14521 gcacgcagtc cggcaggggc cgccatcagc agatcgtgca ggttacgggc aaagacgttg
    14581 attgcttcat cttccgcgcg ttcgcgcacg gtgcccatca gttcagtttc cagatgcatc
    14641 aacaccttga tgcgccacgt ccagctcacc acacctttgc gccagctatc cgccggggca
    14701 ttgttcaggc gcaggccaag gtgatccatg atgatttgct cgcaatagct ctctttcggc
    14761 ggctcttcga actgcggatc ggcattcagc gaaagctgaa gtacgccttc gttacgacca
    14821 cggaacatcg ccagcgcgcg gtgagaaggc accgtggaca gcggttcgtg atgatcgaaa
    14881 tagtcgcgga atttcgcccc ttcctcttct ttaccattca ccacggttga gaccaaatgc
    14941 gcgttcttcc acagataatc acgcactttc gccagcagcg cggcatcttc ggcaaaccgt
    15001 tccattagga tatagcgcgc gccgtccagc gcggctttgg tatctgccac gcctttatcg
    15061 gcatcaatat attgtgcagc ggcgacttct ggcgtgtgtg acggatcgct ccacagcagg
    15121 tcagccaacg gctcaagccc tgcttcaatg gcgatttgcc cgcgggtgcg gcgtttaggt
    15181 ttgtagggca ggtagaggtc ttcgagttcg gttttgctta gtgtggcgtt gatggcattc
    15241 gccagctcgt cagtgagttt gccttgctcg gaaatggact tgaggatcgc ctgacgtctc
    15301 tcttccagct cgcgcagata gctcagacgc gtttccagat tacgcagctg cgtgtcatcc
    15361 agaccgccgg tgatttcctt acgataacgt gcgataaacg gcacggtatt cccttcgtca
    15421 agcaggcgaa cggcagcgtc aacctgttcc gggcgcgcct gaatttcacc cgcaataatg
    15481 cggcagaacg aatcattcat catggtttga gttcatcttt tcggatcaaa aatcagggga
    15541 tagttatacg gactggctgg caaaaatgcc agccatcggc aggaggttaa gactcttcct
    15601 tacggtttca cgtactcgat agcattcaca taccagctcg cttccccggc aggggtattc
    15661 accaccgcca aatcgcccac ctcttttttc aacaatgcgc gagccatcgg ggaatcgata
    15721 gagatgtaat ctttacggcc aaaaatttca tcgtagccga caatacggaa acggtgagtc
    15781 acgccatcgt cgttttcaat ctccacccac gcaccaaaaa agactttgcc ttcctgctga
    15841 ggggagtaat cgacgatttt gagattttcc atgcatttag tgagatagcg cacgcgacgg
    15901 tcgatttcac gcaggcgctt tttattatac tgatagtcag cattttcgct gcggtcgccc
    15961 agacttgcgg cccaggtcac cttttttgtg acctccgggc gttcttcacg ccagagataa
    16021 ttaagctctt gtttgagttt ttcataccct tcccgggtaa ccaggggcgt tttcatctcg
    16081 ttgattccct ttgtctgttt gataatgcgc acattgggta taacgtgatc atatcaacag
    16141 aatcaataat gtttcgccga ataaattgta tacttaagct gctgtttaat atgctttgta
    16201 acaatttagg ctgaaattca taccagattt agctggtgac gaacgtgagc ttttttaaga
    16261 atacacgctt acaaattgtt gcgaaccttt gggagtacaa acaatgcaag agaactacaa
    16321 gattctggtg gtcgatgacg atatgcgcct gcgcgcgctg ctggaacgtt atctcaccga
    16381 acaaggcttc caggttcgaa gcgtcgctaa tgcagaacag atggatcgcc tgctgactcg
    16441 tgaatctttc catcttatgg tactggattt gatgttacct ggtgaagatg gcttgtcgat
    16501 ttgccgacgt cttcgtagcc agagcaaccc gatgccgatc attatggtga cggcgaaagg
    16561 ggaagaagtg gaccgtatcg ttggcctgga gattggcgct gacgactaca ttccaaaacc
    16621 gtttaacccg cgtgaattgc tggcccgcat tcgtgcggtg ctgcgtcgtc aggccaacga
    16681 actgccgggc gcgccgtcac aggaagaggc ggtaattgct ttcggtaagt tcaaacttaa
    16741 cctcggtacg cgtgaaatgt tccgcgaaga tgagccgatg ccgctcacca gcggtgagtt
    16801 tgcggtactg aaggcactgg tcagccatcc gcgtgagccg ctctcccgcg ataagctgat
    16861 gaaccttgcc cgtggccgtg aatactccgc aatggaacgc tccatcgacg tgcagatttc
    16921 gcgtctacgc cgcatggtgg aagaagatcc ggcgcatccg cgttacattc agaccgtctg
    16981 gggtctgggc tacgtctttg taccggacgg ttctaaagca tgaggcgatt gcgcttctcg
    17041 ccacgaagtt catttgcccg tacgttattg ctcatcgtca ccttgctgtt cgtcagcctg
    17101 gtgacgactt atctggtggt gctgaacttc gcgattttgc cgagcctcca gcagtttaat
    17161 aaagtcctcg cgtacgaagt gcgtatgttg atgaccgaca aactgcaact ggaggacggc
    17221 acgcagttgg ttgtgcctcc cgctttccgt cgggagatct accgtgagct ggggatctct
    17281 ctctattcca acgaagctgc cgaagaggca ggtctgcgtt gggcacaaca ctatgaattc
    17341 ttaagccatc agatggcgca gcaactgggc ggcccgacgg aagtgcgcgt tgaggtcaac
    17401 aaaagttcgc ctgttgtctg gctgaaaacc tggctgtcgc ccaatatctg ggtgcgcgtg
    17461 ccgctgaccg aaattcatca gggtgatttt tctccgctgt tccgctatac gctggcgatt
    17521 atgctattgg cgataggcgg ggcgtggctg tttatccgta tccagaaccg accgttggtc
    17581 gatctcgaac atgctgctct gcaggtcggt aaagggatta ttccgccgcc gctgcgtgaa
    17641 tatggcgctt cggaggtgcg ttccgttacc cgcgccttta accatatggc ggctggtgtt
    17701 aagcaactgg cggatgatcg cacgctgctg atggcggggg taagtcatga tctgcgtacg
    17761 ccgctgacgc gcattcgcct ggcgaccgag atgatgagcg agcaggatgg ctacctggcc
    17821 gaatcgatca ataaagatat cgaagagtgc aacgccatca ttgagcagtt tatcgactac
    17881 ctgcgcaccg ggcaggagat gccgatggaa atggcggatc tcaacgcagt gctcggtgag
    17941 gtgattgccg ccgaaagtgg ctatgagcgg gaaattgaaa ccgcgcttta ccccggcagc
    18001 attgaggtga aaatgcaccc gctgtcgatc aaacgtgcgg tggcgaatat ggtggtgaac
    18061 gctgcccgct atggcaatgg ctggatcaaa gtcagcagcg gaacggagcc gaatcgcgcc
    18121 tggttccagg tggaagatga tggtccggga attgcgccgg aacaacgtaa gcacctgttc
    18181 cagccgtttg ttcgcggcga cagtgcgcgc accattagcg gcacgggatt agggctggcg
    18241 attgtgcagc gtatcgtgga taaccataac ggcatgctgg agcttggcag cagcgagcgg
    18301 ggcgggcttt ccattcgcgc ctggctgcca gtgccggtaa cgcgggcgca gggcacgaca
    18361 aaagaagggt aaataaaacg ggaggcgaag gtgcctcctg tttttagatt gttttcaatt
    18421 gattgataat gaaaggattc gcttgcaaaa gcaggagcaa ttttcggggt gctccggtag
    18481 gttgacggcg tttggtttcc cagcttttga ccaggtcata acttacccct acggcgacag
    18541 caaaatcttg ctgacgtaaa cccgttgctt cacggatcgc ttttacatcg atttcgctaa
    18601 aagtatggac gtgctctggt tcaggcgttt cttcaccctt ttcgatgcgt accatttctt
    18661 ctgcgctggc aagaagatca gcaaataatt catctttcat ctgttttcaa tgcctccgtt
    18721 gacatttcgg ccatggtgga atttgtggag cttcaacatt aaagcagctt ctcggacaat
    18781 agtttcaatg cctttctttg ttcttctgtt aaatcatcct gttcattttt aggataaatc
    18841 aacgccagat aaattcggcc attgcgtgtt acgttgtaat aaattatccg aatgccaccc
    18901 cgtttcccca ttccagaacg gttccagcga atttttctaa aactgcctgt gcctgcgata
    18961 atgtcaccgg cagtgggatc ttgaatgagt agttcctgaa aagcccggaa ctcatcatca
    19021 ggtagcaata actggcggcg tttgctaaat ccttgtagtt caataaaggt gaacataatg
    19081 catcctgcgg tctattgata attataagtg taccgtgtac atattggtag ctaattagat
    19141 aaaaaaaccc cttatccgtt tggataaggg gtcaatgaga tatcagctga ttacagctta
    19201 ggacctgccg ctaccagcgc ggcacccgca ggggtgtcgg tgtatttatc gaagttgtcg
    19261 ataaacagtt tcgccagcgt ttcagctttt tcctgccact gttccggaga ggcgtaggtg
    19321 ttacgcggat cgaggatcct ggtgtctacg cccggcagtt cggttgggat cgccaggtta
    19381 aacatcggca gagtgaaggt ttctgcgtta tccagcgaac cgttgaggat ggcgtcgata
    19441 atggcgcggg tatctttaat ggagatacgt ttgccagtgc cgttccagcc ggtgttaacc
    19501 agatacgcct gcgcgcctgc cgcctgcata cgtttcacca gcacttctgc gtactgcgtc
    19561 gggtgcagcg acaggaatgc cgcgccgaag caggcggaga aggttggcgt cggttcggtg
    19621 atgccgcgct ctgtaccggc cagtttggcg gtgaagccag agaggaagtg gtactgggtt
    19681 tgatcggcag tcagacgaga aaccggcggc agcacaccaa aggcgtctgc ggtcaggaag
    19741 ataactttag ttgcgtggcc cgctttggac accggcttaa cgatgttttc gatgtgatag
    19801 atcggataag aaacgcgggt gttctcggtt tttgaaccat catcaaagtc gatagtgcca
    19861 tcttcacgca cggtgacgtt ttccagcagc gcatcacgac ggatagcgtt gtagatttca
    19921 ggttccgctt ctttcgacag cttgatagtt tttgcgtagc agccgccttc aaagttaaac
    19981 acaccgtcat cgtcccagcc gtgctcgtca tcgccaatca ggcgacgttt cgggtcggtg
    20041 gaaagggtgg ttttaccggt gccggaaagg ccgaagaaca ccgcaacatc gcctttctcg
    20101 ccaacgttgg cggagcagtg catagaagcg atacctttca gcggcagcag gtagttcatc
    20161 attgagaaca tccctttctt catttcgccg ccgtaccagg tgccgccaat cagctgcatg
    20221 cgctcggtca ggttaaacgc cacgaagttt tcggagttca gaccctgttc tttccactgc
    20281 gggttagtgc acttcgcgcc gttcataacg ataaagtctg gtttgaaacc tgccagctct
    20341 tcatcgctcg ggcgaataaa catgtttttg acaaaatgcg cctgccaggc cacttcggtg
    20401 atgaaacgga cggaaagacg agtatccggg ttcgcaccac agaaggcgtc gacaacgaac
    20461 agacgtttgc cggaaagctg tttggtcacc aggcctttca gatgctgcca ggtttccgga
    20521 gagagaggtt tgttgtcgtt cttacctttg cctttgtctg cccaccagaa agtatcgcga
    20581 gtggtatcgt cacggacgat atacttatct tttggtgaac gaccggtgaa gatcccggta
    20641 tcgacggcaa cggcacccag attagttaac accccgcgct cataacctgt caggctcgga
    20701 tcgagctctt cctgatacag caggtcgtag cttgggttgt aaacgatatc atgtacgtca
    20761 ctgataccat aagcctcgag ttcttgcggg gtcaaaccat tgttaacgcg catttcactg
    20821 ctccttagcc aatatgtatt gcctgaatag taaagtcttt ttgggggtgt taaccgcgac
    20881 aaggctcata gatttacgta tctggagaaa ttcatatttg acaaaaaact ccgtgattcc
    20941 tgtcacgaaa cggttgctat tatcgcagaa atagcatttt aggtgtggaa aatgttctta
    21001 aaaggttaaa tcttggttta tatggcggga atattgtgag tggaatcgca ttcctgaatg
    21061 aaattgatgc aaattcaatc ggtaaagtat cgattcacca cgctaacttt ttttgattat
    21121 tttctgaaaa aagcaaaata ctacggcccg gatgcaataa ttaacccact ttttgattct
    21181 ggataactaa catggataac gttgaacttt caccggcaac acgctggggg atgattgcta
    21241 ccggattact tcagggtctg gtttgctacc tgctgatcgc ctggcttgcc ggaaaaaatc
    21301 acagttggat tgtttatggc gtgcctgcga ccgtcgcgtt ttcatccgtt ttactcttct
    21361 ccgtgatctc ctttaaacag aagcgccttt ggggatggct ggcgctggtg tttattgcca
    21421 cgctagggat gagcggttgg ctaaagtggc aaattgatgg catgaacccc tggagagcag
    21481 aaaaggcgct ttgggatttt ggttgctatc tgctgctgat ggcaatgttg ttgctaccgt
    21541 ggatacaaca aagccttcgc atccgcaatg acagtagccg ctatcgctat ttttaccagt
    21601 cagtatggca taacgtactt atattactgg tgatttttct cgccaatggc ctgacgtggc
    21661 tggtactttt actgtgggga gagttgttta aacttgttgg catcacgttt ttcaaaacgc
    21721 tcttttttgc aaccgactgg tttatttatc tcacgttagg tctggttacc gcactggcgg
    21781 tgatcctcgc gcgtacacag tcacgtttaa tcgactctat tcaaaagttg ttcacgttaa
    21841 tcgccacggg gttgctgccg ttagtatcat tgctaaccct gatgtttatt atcaccctgc
    21901 cgtttacggg cctgagcgcg atttctcgcc acatctccgc cgctgggttg ctgttaacgc
    21961 tggccttttt gcaattgatc ttaatggcta ttgtccgcga tccgcaaaaa gcgtctcttc
    22021 cctggacggg gccgttgcgt tgcctgatta aaaccgcttt gctggttgct ccgctgtatg
    22081 tgttcgtcgc cgcctgggcg ttatggctgc gggtcgctca gtacggctgg actgtcgacc
    22141 gcttgcaggg cgcgctggcg gtgctggtgt tactggtatg gtcgctgggg tattttgtca
    22201 gtatcgtctg gcgtaaaggg caaaaccccc ttgttcttca gggcaaagtg aaccttgcgg
    22261 tttcgttatt ggtgttggta atactggtgc ttcttaattc tccggtgctg gacagtatgc
    22321 gcattagcgt gaacagtcat atggcgcgtt atcagagcgg caaaaacacg ccagaccagg
    22381 taagtctcta tatgctcgaa cagagcggtc gctatggacg tgcggcgctt gagtcgctga
    22441 aaagcgatgc cgagtatatg aaagacccga aacgcaggcg gggtctgctg atggcatttg
    22501 atggagtaca acgtcttcag cagcagatat cggaaaaagc attagcagat aatgtattta
    22561 ttgcctcagg ttctggaaaa ccggatacga tattctggtc ggcagtgatg aaaaatcgct
    22621 ataacgcaat gatatgcatt gaaaaagatg cctgcgtgct ggtagagcaa gatctgaata
    22681 gtgatggcag ggcagagcgg atcctgtttg cctttaatga tgaaagatac attatctatg
    22741 gtattgatcc tgctgggaaa gaatggaaag agattacgat gaattcactc ccggctgtaa
    22801 taacgaaaga aaaattactc atggctgcgg aggatggaaa actggggacg aagcctaaag
    22861 tgtggcgcga tcttacggtg gatggcgaaa gactggattt gaatctgaat gaatgacagc
    22921 gcatatctga tttttattgg attcggcaca ttatcttacc tgcgataaat tgcattgaat
    22981 ccgtaggtct gataagcgca tcaggccatt acgtcgtaaa atccggcaga gccctctctg
    23041 ccggacatac tcattaatga acttgcggat ctgccggaga cgcgttgttg cggatttcag
    23101 caatatccat cgcattgaac agatagtggt taccgcagta atcacaatgc atgtcaattt
    23161 cgccatcttc cgccaggatg ctatcgactt cttcatccgg cagcgttttt agcgcgtcgg
    23221 cgcaacgttc acgcgagcag gtgcatttga actccacatc ctgcggatca taaaccgtca
    23281 cctcttcttc gtgatacaaa cgccacaaca cttcgtttgc cggtaaggtc aacagttctt
    23341 cggttttgat ggtttcggtt agcgtcgcca ggtggtcaaa gtcgtcctgc tgggcatttt
    23401 gcgcaggcat tacctgcaac aacataccgc ctgcagccgg tttgccgtct acgtcgccgg
    23461 tgcgaataaa caggcgcgtc ggcagctgtt cagaacgcat aaagtaatct tccaggcagg
    23521 ccgccagagt atcaccttcc agacccacca cgccctgata gcgttcacct tcgctcgggg
    23581 tgatggtgat caccacgtaa ccattgccga ccagcgtttt caggtcggca ttttctggaa
    23641 tttcgccctg cacgcgcgcc acaccgcgca tctgctggtt gttgttaccg ttaataaccg
    23701 ccagattcat cggaccgtcg ccttgcagct gtacggtgat atcaccatca aacttcagcg
    23761 tagcggttaa caggctggtc gcaaccagca gttctgccag cacgttttta acgggctgcg
    23821 gataatcgtg gttctcaagg atctgttgca gggtttccga aacggttacc agttcgccgc
    23881 gaacggcaaa gttttcaaac agatagcgat gtaattggtc atgttgcggc ataatcatct
    23941 ctcttgcagg tgacagttat tcactgtcgc cgtgtttaaa tcgtaacagg tcgcggcgct
    24001 cttttttgtc cgggcgtcgg tccgggtgcg gcatggttaa ggcattaagt ttacgtgcca
    24061 gcgccatttt ttcgcgtttc tctacacttt ccgcagtctc ttcatacagc aaggctgcct
    24121 cgctggcggg gcgacgctgt tcagtaatcg cctttacaat caccgtgcgt tcgtcatttc
    24181 cctggcgcag agtgagcgtg gcattcagct cgacgatttt gctcggcttg ctgcgctgcc
    24241 cgttgtaatg caccttaccg ccttcaatca tttcacgggc cagcgcgcgg gttttataaa
    24301 aacgggcagc ccatagccat ttatccagtc gaacctcaac agcaggtttt tctttcatgg
    24361 cgtctccttc acattagcga ggggatcagg cggcggtagt cattcagtga cggatggcgt
    24421 tgatactgtt tctcggcaat cccggagtca ggattagtca cgccgaggca gtaacgaata
    24481 ccaaattgcg cggcagcatc gagaatcgct tcgctgtcat caataaacag cgttctttca
    24541 gctttcagac ccgtagcttc ggccaccgca tgccataacc gctgatcctc tttcggataa
    24601 ccaaatgtgt gggtggaaag taataaatca aggtgtgcgt ccagaccggt atgctcaagt
    24661 tttaccgcca ggttgtgcgg atgcgcattg gtgagcaaaa ttcgctgctt accgctggct
    24721 ttcagtgcct caagaaacgg aatggtatct tcacgcagta cggcgcgcgg tcccatctcg
    24781 gtggtcatcg cacagatatc cagacccagt tgctcactcc agtaatcaag acagtaccag
    24841 tttagcgtat gctgtacgtc gtgatattgc tggcgcatat attccatcgc ttcctgtggc
    24901 gtaaccccgt ttttcgcgcc ccatgtttca ggcaccagct tttgccagaa atagttatcg
    24961 aaggcgaggt cgagcaacgt gccgtccata tccagcagaa cggtatctac gtcctgccag
    25021 gcaatgttga tatgcatgag ggaaatctcc agagtgaagc aatttgcgcg acagggtagc
    25081 ataacctgcc gcgcaaacgt gttattcgat aaggctttct gaaggggtga tcagttgtgg
    25141 gttcaggcag ctttcataat aggcctgaat cttcatcatg cgagtacgat gacgctggta
    25201 acgccgccag gcctgtacgc cattataaat ggcgctaccc agcatcgtca gtagcagcag
    25261 cgtggtgctg agatagcgcc acaggccgga acgatccggg atcggatgaa ggcctatatg
    25321 ctgcgtacca ttggcgtcag tgaagatttt tgtgacgata ccctcggcgt taaacggcgt
    25381 atgcatcagc atttgtgcca gtttctggaa agcgttccac tgttcttgcg gcgggtagtc
    25441 gtaaagtgat gccgaaggcc agggctgatc aacaaaatcg ctgccttcgt cgctgacaat
    25501 caggaatccg ccgggggccg gactgtttag tgattgtgcc gctcgcgccg tttcatgcgt
    25561 gataaacggc gcggtggagg ttgccaccag gttatccagc gattccgcac tcaccgggcg
    25621 taataacaca ttcacgccat cgagtttccc ggcgttggcg cgttttacca gcgcgtccca
    25681 gtctttactg ttgccgagat tgaccagcgc atttttcagt cgcacacagt catctttggc
    25741 agaacacaga tccgctgtct tcagtactat gtcgccaaaa tcatcaagca ataccatgcc
    25801 ggatttttga attgctgaac gtaatgaggc gctgacgcga gattcatctt ccggtttagg
    25861 gtgcagctgg cgattaactg cttcggtcaa tgccgtcgct ttgttgacca attcagattc
    25921 tggtaatggc aatgagcggg cgtcattcca gatgatctgc gagcagtcaa acggtaaaaa
    25981 aggtgagttg gttttcgcgc tccaggtacc ggaagtgcga atattacaca ttcccgtacc
    26041 gctaatacgc aatgtatcgc caacccgtac gccagcgtca gccagttgtt tcacgctggt
    26101 ggcttcaatg gtctgcgcgc ctttcatcca tgagagagtg aatttcagcg gcatatccag
    26161 cgggatccag aataacagca taaacagcac cagcagcgaa cccgcagcga taatcgtact
    26221 gcgcagccag tgctgtaacg gaaagttttt tacttcatca tgcagcgaaa gatatcgccc
    26281 ctgacgcact acgtggcggt cgagatagat atcgatatcg gtttgttggc cgagatcatg
    26341 agcaatgtag ggctgccagt gtgcggggta gaccaggtcg ataataccga gtgaaatatt
    26401 gttgatctgt tcctgatcgt tttcgccaaa caatccccaa cgacggggtg taccgcgcag
    26461 acaatgaatt tcccgcaggg aggattttgc cgggggcgcg aatagccccc acagacctgc
    26521 gcccagcagc agtaacgcgc cgcctgccag ccacggaaca aatacatccg gggtaatcag
    26581 gcagaaaaag aacatcagga aggaggcgac aatcagcaac gcttcgcgca gcccgcgcgg
    26641 acgacttagc gcatactctt cgtgcgtttc tttgcgaata ttgagtagct cgatttgctc
    26701 actctcttcc ccgcgaatcg acgcctgtgt tgacggaaca ggttgcaagg catagccgcg
    26761 agtttcctgc atgtactcct gcagcgtatg accgttgagt gaaataacca gcggcagcga
    26821 atcggtatga atcagttcaa cggtattctc atcgttgatg tactgctccc agaaaggggg
    26881 caggtggact tctaccgaat cgaggtagta acgccattta ttcggatcgt cggtagagat
    26941 gccgtaacgc gtgatagcgt gtgttagcat catgacgttg ttgctttcgg cattcagcgc
    27001 cagagaaatc ggtgccgcgc tggctcccgt tgggccaggc acctgtaata cctgcgtcag
    27061 gctctcaaga taattttcaa cggcgctacg ttcttcaggt gtgagtttac gcgtttgcgc
    27121 atccgcgaag gcgttggtcc agggcagctg acgccgtctg gatcgcactt ttatcagcca
    27181 tcccgcaagt agtgagcagg ccagcaaagc agctaaaaaa atcacaatgg tgctcatgct
    27241 ttccccatct tacttagtct gtcaggcgtg gtcagtcgca ccggacacaa aacatgattc
    27301 tgcggttggc tatcggcaag cgtggagttt aacttgagca tcattaagca catcacagta
    27361 accgctgatc atagcaaacc ctctgaaggc ggaatatcag caaattcctg gagttatttc
    27421 aacctattga cttttaattt gatttgagga cgattattta ttaagttaca taaatgtaaa
    27481 taacatgcaa ttcacattgc cgaaagcgtg tccgatatcg cacaataacc gctgatttca
    27541 cagcatagat cccacgatga gcaaatcatt acaaaaaccc accattctga atgttgaaac
    27601 tgtagcccgt tcccgactgt ttaccgtcga aagcgtggat ctggagttca gcaatggcgt
    27661 gcggcgtgtt tatgaacgaa tgcgtccaac caaccgggaa gcagtgatga ttgtgccgat
    27721 tgtggacgat cacctgatcc tgatccgcga atacgcagtg ggaactgaat cctacgaatt
    27781 aggtttttcg aaaggattaa ttgatccggg tgaaagcgtc ttcgaagccg cgaaccgcga
    27841 gctaaaagaa gaggtgggat ttggtgcgaa cgatctgact tttttgaaga agctcagcat
    27901 ggcaccgtct tacttttcca gcaaaatgaa tatcgtggta gcgcaagatc tctacccgga
    27961 atcgctggaa ggcgatgagc cagagccgct accacaggtg cgctggccgc tggcgcatat
    28021 gatggatttg ctggaagacc ctgacttcaa cgaagcgcgt aatgtcagcg cgcttttcct
    28081 cgtgcgcgaa tggttgaagg ggcaggggcg gttgtaaatg cgtgaagttt gcctgatgca
    28141 taatgcttat caggcctgtg tggctcctgc aatatattga tttttctaga tgtttttaga
    28201 cgggtaaagt gttcacgccg tatccggtat aaacaaagtg cactttgtca gcaaacttaa
    28261 aaaggcgctg aagcgccttt ttaatcagaa caattcctgt gcctcgccat tatcgataat
    28321 ggtcgttccc acctcgtgca ccgcctgttg tgtcggctgc gtaccttcga tgaaatactc
    28381 ttcgcggctg ttgccaccat tagctaactg cccggtgctg cgatcgatat tcaccgtcac
    28441 aatacccggc ggcggcgtca gcggctgctc cggcacacct tcaagaacgg ctttcatata
    28501 agcgtcccat gcaggttggg cactcttggc accgccttcg taacctgaga tctgatcttt
    28561 aatcgctccg gaagctgttg tatgaccgag attacgacgg tgatcatcaa agccaatcca
    28621 gaccgaggtc acaacgcccg gaccgtaacc cgagaaccac gcatctttcg aactgttagt
    28681 ggtacccgtt tttccgccta tatcatgccg ttgtaaatca cgacctgctc gccagccagt
    28741 accctgccag ccgggttcac caaaaatatt ggtgttcaag gcactcttaa tcaggaatgc
    28801 cagcggagtg ttgatgacgt gcggtgcgta ttcctgcgcg ccggtcttcg ccactaacgc
    28861 ctgatttgcc tgctccagct gcggcattgg tacagaaaca ttctgctgct cgcgggagat
    28921 agcgacatct tcaacatcgt tattttccag cacgttcgat ttctgcgtat caccgtaaat
    28981 caccggaata tcgcattccg ggcaggctac tttcggtttc gcttcgaaaa tcacgccgcc
    29041 ctgatcgttt tcaattttgc tgataaacca cgggtccacc aggaagccgc cgttcgccat
    29101 gaccgcgtag ccgcgcgcca cctgcattgg ggtgaatgac gctgaaccca gcgccagcga
    29161 ttcggtgtgg acaatgtttt gcgccgggaa gccgaagcgt tgcagatatt ctgcagcgta
    29221 gtcgacgccc atcgcccgca ttgcgcgtac catcaccacg tttttcgact ggcccagtcc
    29281 ctgacgtaag cgaattggac cggcatactg cggtggtgag ttcttcggct gccagtcaga
    29341 accggcacct gcatcccagc gggaaattgg cacatcgttc aacatacttg ccagcgtcag
    29401 acctttatcc atcgccgcgg tgtagaggaa cggtttgatg ttggaaccta cctgacgcag
    29461 tgcctgggtg gcgcggttaa acttgctctg attgaaatca aagccaccga ccagcgccat
    29521 aacggcaccg ttttgcggat tgatcgacac cagcgccgag ttcacttccg gcacttgcgc
    29581 cagccaccat gcatcgccaa cctgacgaac ccagatttgc tgacccgttt gcagaacatc
    29641 ggtcacttta cgcggcgtcg gtccctgctg agtatccgaa cggtaaggac gcgcccagcg
    29701 aacgccttcc atactcaatg cgacggtcga cccgtccgcc agcatcgccg tcgcttcctg
    29761 aggattggcg ctggtgactg cggcaggcag cagcggacca taggtcggca gcgccttcag
    29821 cgtatcggta atcttgttgt tatcccacgc cgactcgccc actttccaca gcacatttgc
    29881 cgggccgcga tagccgtggc gcatgtcgta gtccagcacg ttattacgta ccgcctgctg
    29941 tgcggcctgc tgcactttgc gggtgatggt ggtgtaaatg cgataaccgt cttcataggc
    30001 actttcgcca taacggttat acatctcctg gcgcaccatt tcgctcaggt acggcgcaga
    30061 gaaagcaatc tccggcgcgt gatagttagc gttaatcgcc tcagtgcgtg tctgatcgaa
    30121 ctgttgttgg gtgatatacc cttcatccag catccgcgac agtacgacgt tacgccgcgc
    30181 gacggcacga tccattgagt agagcggatt aaaggtggaa ggcgctttcg gcagcccggc
    30241 tatcaccgcc atttcgttca gcgtcagttg gtcgaccgtt ttaccgaaat agacttgtgc
    30301 cgcagcaccg acaccatagg cgcggtaacc gaggtaaatc ttgttcagat aaagctcgag
    30361 gatctcgtct ttcgtcagca actgttcaat gcgaatcgcg aggaagactt ccttaatctt
    30421 acgcatcagc gtgcgttctg gactgaggaa gaagtttctc gccagctgct gggtaatggt
    30481 actcgcccct tgtgacgcgt ggccggagaa cagcgccaca cttgccgcac ggaagatccc
    30541 caccgggtca acgccgtgat gctcgtagaa gcggctgtct tccgtcgcga taaaggcttt
    30601 caccatctcc ggcgggattt gatccaacgt aaccggaata cgacgtttct caccgtattg
    30661 agcaatcagc tcgccatcgg cgctgtaaat ctgcatcgga atttgcaggc gaacatcttt
    30721 taatgtcgcc acatccggca gttgtggctc gatgtagcgg tataggccat aaatcgagcc
    30781 tgctcccagc agaatgcaac agactgcaag gatcaaaaaa tactttacga acttcactgg
    30841 aaatttccca tttagtttca tttgggcagt ttataaacaa acgcgcggta gtataaaggc
    30901 aagccagacg cattgatata cccgtcagag tgacgggtga taaggagatc atcacaatgg
    30961 catttaagat ctggcaaatt ggtttgcatt tacaacagca agaagcggta gcggttgggg
    31021 cacaaaagaa tgctttttgc aacgctggtg gcggttgccg ctggagaacg acattatcaa
    31081 agatgggcgg attgttgatg tgcagcggct ggctaaaacg ttgttaccgt ggagtcgcga
    31141 actgccgcag cgtcatcaca ttatgttggc gtttcccgcc agtcgcacat tacagcggtc
    31201 atttccacgt ccgtcgatgt cccttggtga gcgggagcaa acggcctggc tgtccgggac
    31261 gatggcccgc gagctggata tggatccgga ctccctgcgc ttcgattaca gcgaagactc
    31321 actcagtccc gcctataacg tgactgccgc gcaaagcaaa gagttggcaa cgctgcttac
    31381 tctggcagaa aggttgcgtg ttcatgttag cgcgatcacc ccggatgcca gtgcattaca
    31441 gcgattcctg ccttttttac cttctcatca gcaatgtctg gcctggcgtg ataacgaaca
    31501 gtggctgtgg gcgacgcgct atcgctgggg gcgcaaactg gcggtgggga tgactagcgc
    31561 gaaggagctg gcggcagcgt tatccgttga tcccgaaagc gtcgcgatat gtggcgaagg
    31621 cggatttgat ccctgggagg cggtttctgt tcgtcagccg ccgctaccgc cgccaggtgg
    31681 agactttgcc atcgcgctgg ggctggcgct agggaaggcg tactgatgaa cccgccaatt
    31741 aattttttgc cctggcgaca gcaacgccgg accgcttttc tgcgtttctg gttgctgatg
    31801 ttcgttgcgc ctctgctgct ggctgtcggg ataacgctaa tactgcgtct gacaagcaac
    31861 gccgaagcac gcgtagacac cgttttgctt caggcggaac aacaactcgc ccgcagctta
    31921 cagataacga aaccacgttt gctggaacgc cagcagttac gcgaacagcg tttgcaaagg
    31981 cagctccagc gacaatttac ccgtgactgg caatctgcgc tggaagcact ggcggcgctt
    32041 ttacccgaac atgcctggct gacaacgata agctggcagc aaggtacgct ggagatcaag
    32101 gggctgacaa caagcattac cgcgttaaac gcgctggaaa cgtcactccg ccaggatgca
    32161 tcttttcatc tcaatcagcg gggggccacg cagcaggatg cgcagggacg ctggcaattt
    32221 gagtatcagt taacaaggaa ggttagcgat ggacatgctc tttgactggt ggttcgccac
    32281 atcaccccgc ctccgccagc tttgctgggc agtctggttg ctgatgttag ttacgctcat
    32341 ttttctgtca tcgacacacc atgaagagag cgacgcatta attcgactac gggcaagtca
    32401 tcaccagcag tgggccgcac tgtatcgcct ggttgacacc actcccttca gcgaggaaaa
    32461 aacgctgccc ttttcgccac tggattttca gttatccggc gcgcaactgg tttactggca
    32521 tccatccgcg cagggaggtg agttggcgtt gaaaacgctg tgggaagcag tgccgtcggc
    32581 atttacacgg ctggcagagc gcaacgtcag cgtgagccgt ttttcgttaa gcgtggaagg
    32641 tgatgacctt ttgttcacgc tacaactgga gacgccgcat gagggttaaa cgctggttgt
    32701 tggcaggtat tacattgtgc cttttaaccg gtatgcgtga cccttttaaa ccgccggaag
    32761 atctatgccg gattagcgaa cttagccagt ggcgctatca ggggatggta gggcgaggtg
    32821 agcgcatcat cggtgtaata aaagacgggc aaaaaaaatg gcgacgggtg cagcaaaacg
    32881 acgtgctgga aaacggctgg acaattttac agctgacgcc agacgcacta acgctgggaa
    32941 ccgggacaaa ctgcgaaccg ccacactggt tgtggcaacg gcaaggagat acaaatgaag
    33001 caatggatag ccgcactact gttgatgctt atacccggcg tacaggcggc aaagccgcaa
    33061 aaagtgacgc tgatggtgga tgacgttccg gtagctcagg tgttgcaggc gctggctgaa
    33121 caggagaagt tgaacctggt ggtttcgcca gacgtcagcg gtacggtgtc gttacattta
    33181 actgacgttc cctggaagca ggcactacaa actgtagtga aaagcgccgg attgataacg
    33241 cgccaggagg gcaacattct ctcggtgcat tccgttgcct ggcagaatga caatatcgcc
    33301 cgtcaggagg cggagcaggc gcgggcgcag gcaaatctgc cgctggaaaa tcgcagtata
    33361 accctgcaat acgccgacgc gggagaactg gcgaaagccg gggagaagct actgagtgcc
    33421 aaagggagta tgaccgtcga taaacgcacc aatcgccttt tgctgcgaga taacaaaacg
    33481 gcgttaagcg cgcttgaaca gtgggtagcg caaatggatc tgccggtcgg gcaggttgag
    33541 ctgtcggcgc atattgtcac cattaatgaa aaaagtttgc gtgagttagg cgtgaaatgg
    33601 acgttggctg atgcgcagca agctggtggc gttgggcaag tcaccacgct tggcagcgac
    33661 ctctccgtag cgacggcgac aacgcatgtc ggttttaaca ttggacgcat caacggacgt
    33721 ttactggatc ttgagctttc cgcgctcgaa caaaaacagc agctggatat tatcgccagt
    33781 ccgcgtctgc tggcctcaca tcttcagcct gccagcatta aacaggggag cgaaattcca
    33841 tatcaggttt ccagcgggga aagtggcgcg acgtcggtgg aatttaaaga ggccgtcctg
    33901 gggatggaag ttacgcccac ggtgttacaa aaaggtcgca tccggctgaa attacacatc
    33961 agccagaacg ttccggggca ggtgctacag caggccgatg gcgaagtgtt ggcgattgat
    34021 aagcaggaga tcgaaacgca ggtcgaggtc aaaagcggag aaacgttggc gctgggcggc
    34081 atttttaccc gtaaaaataa atcgggtcag gatagcgtac cgttgcttgg cgacattccc
    34141 tggttcgggc aattatttcg tcatgacgga aaagaagatg aacgacgcga gttagtggtg
    34201 tttatcacgc cacgactggt ttccagtgag taaacagccg taaaagcggt aatgttttta
    34261 cgctgaacgt gtttcatcta tttgacgcgc gcaggtattt agcatacaag gagtaccgat
    34321 ttgagagttg gtgctcttcg ctgcctgcgt tccatgatga tgatttatca ttcaggcggc
    34381 attttgctgt cttttttacg ctaatcttac ccggtgatta atcgccagag cggtggtagc
    34441 aaggcagcgc gcttgcagcg accagatatg cagagggatg ggtgatttat tcagttgcca
    34501 aacccgctgg agtattgaga taattttcag tctgactctc gcaatatctt atgaggtttc
    34561 agttcatgtc ctgcggcgct ctctgagcga agcgggttta tcattaacga atagtcttag
    34621 tagtaccgaa aaaatggcag agaaacgcaa tatctttctg gttgggccta tgggtgccgg
    34681 aaaaagcact attgggcgcc agttagctca acaactcaat atggaatttt acgattccga
    34741 tcaagagatt gagaaacgaa ccggagctga tgtgggctgg gttttcgatt tagaaggcga
    34801 agaaggcttc cgcgatcgcg aagaaaaggt catcaatgag ttgaccgaga aacagggtat
    34861 tgtgctggct actggcggcg gctctgtgaa atcccgtgaa acgcgtaacc gtctttccgc
    34921 tcgtggcgtt gtcgtttatc ttgaaacgac catcgaaaag caacttgcac gcacgcagcg
    34981 tgataaaaaa cgcccgttgc tgcacgttga aacaccgccg cgtgaagttc tggaagcgtt
    35041 ggccaatgaa cgcaatccgc tgtatgaaga gattgccgac gtgaccattc gtactgatga
    35101 tcaaagcgct aaagtggttg caaaccagat tattcacatg ctggaaagca actaattctg
    35161 gctttatata cactcgtctg cgggtacagt aattaaggtg gatgtcgcgt tatggagagg
    35221 attgtcgtta ctctcgggga acgtagttac ccaattacca tcgcatctgg tttgtttaat
    35281 gaaccagctt cattcttacc gctgaaatcg ggcgagcagg tcatgttggt taccaacgaa
    35341 accctggctc ccctgtatct cgataaggtc cgcggcgtac ttgaacaggc gggtgttaac
    35401 gtcgatagcg ttatcctccc tgacggcgag cagtataaaa gcctggctgt actcgatacc
    35461 gtctttacgg cgttgttaca aaagccgcat ggtcgcgata ctacgctggt ggcgcttggc
    35521 ggcggcgtag tgggcgatct gaccggcttt gcggcggcga gttatcagcg cggtgtacgt
    35581 ttcattcaag tcccgacgac attgctgtcg caggtcgatt cctccgttgg cggcaaaact
    35641 gcggtcaacc atcccctcgg taaaaacatg attggcgcgt tctaccagcc tgcttcagtg
    35701 gtggtggatc tcgactgtct gaaaacgctt cccccgcgtg agttagcgtc ggggctggca
    35761 gaagtcatca aatacggcat tattcttgac ggtgcgtttt tcaactggct ggaagagaat
    35821 ctggatgcgt tgttgcgtct ggacggtccg gcaatggcgt actgtattcg ccgttgttgt
    35881 gaactgaagg cagaagttgt cgccgccgac gagcgcgaaa ccgggttacg tgctttactg
    35941 aatctgggac acacctttgg tcatgccatt gaagctgaaa tggggtatgg caattggtta
    36001 cacggtgaag cggttgctgc gggtatggtg atggcggcgc ggacgtcgga acgtctcggg
    36061 cagtttagtt ctgccgaaac gcagcgtatt ataaccctgc tcacgcgggc tgggttaccg
    36121 gtcaatgggc cgcgcgaaat gtctgcgcag gcgtatttac cgcatatgct gcgtgacaag
    36181 aaagtccttg cgggagagat gcgcttaatt cttccgttgg caattggtaa gagtgaagtt
    36241 cgcagcggcg tttcgcacga gcttgttctt aacgccattg ccgattgtca atcagcgtaa
    36301 caacaagaaa ggtcaggccg cttatcaagc ggtctattag cttcaggtta attgcaacgt
    36361 ggtaagcatt aaccttttag tggggtgtta aatggatgaa ttcaaaccag aagacgagct
    36421 gaaacccgat cccagcgatc gtcgtactgg tcgttctcgt caatcttctg aacgttctga
    36481 gcgtactgaa cgtggcgaac cgcagatcaa ttttgatgat attgaacttg atgacactga
    36541 cgatcgccgt ccgactcgtg cgcaaaaaga gcgcaatgag gaaccggaac tcgaagaaga
    36601 aattgacgaa tccgaagatg aaaccgtgga tgaagagcgc gtagagcgtc gtccacgtaa
    36661 gcgcaaaaaa gcagccagta aacccgcttc tcgtcagtat atgatgatgg gcgtcggcat
    36721 tctggttctc ctgctgttga tcatcggtat cggttctgcg ctaaaagccc cctcgacctc
    36781 ttccagcgat caaaccgcgt ctggcgagaa gagtattgat cttgctggca atgcgaccga
    36841 tcaggcgaat ggcgtgcagc cagcgccggg aaccacgtct gcggaaaata ctcagcagga
    36901 tgtttctctg ccgccgatct cttctacgcc gactcaaggg caaaccccgg cggcaacgga
    36961 tggtcaacaa cgtgttgaag tgcagggtga cctgaacaat gcgctgaccc agccacaaaa
    37021 tcagcaacag ttgaataatg tggcggtcaa ttccacgttg ccgactgaac ccgcgacggt
    37081 cgcgcctgtt cgcaatggca atgcatcgcg tgacacggcg aaaacgcaaa ccgctgaacg
    37141 tccggccact acgcgtccag ctcgccagca ggcggtgatt gaaccgaaaa aaccgcaagc
    37201 aaccgtgaaa acggagccga agccggtagc acagacgccg aagcgtactg aaccagctgc
    37261 tcctgtggca agcacgaagg caccggctgc gacttctacg ccagcaccaa aagagacggc
    37321 gactacggct ccagtacaga cggcatcccc ggcgcaaacc acggcaacac cagccgctgg
    37381 agggaagacc gcaggtaatg ttggttcgtt gaaatcggca ccgtccagcc attacactct
    37441 gcagctgagc agttcctcta actacgacaa cctgaacggt tgggcgaaga aagagaatct
    37501 gaaaaactac gttgtctatg aaacgacgcg taatggtcag ccgtggtatg tcctggtttc
    37561 tggcgtgtac gcttcgaaag aagaggcgaa aaaagcggta tctacattgc cagccgatgt
    37621 ccaggccaaa aacccgtggg cgaaaccgct gcgtcaggta caggccgatc tgaagtaatc
    37681 aaggttatct cccgcaatgg tttatcatcg cgggagttgc ctgaagcgct ggatgctgtc
    37741 ggagctttct ccacagccgg agaaggtgta attagttagt cagcatgaag aaaaatcgcg
    37801 cttttttgaa gtgggcaggg ggcaagtatc ccctgcttga tgatattaaa cggcatttgc
    37861 ccaagggcga atgtctggtt gagccttttg taggtgccgg gtcggtgttt ctcaacaccg
    37921 acttttctcg ttatatcctt gccgatatca atagcgacct gatcagtctc tataacattg
    37981 tgaagatgcg tactgatgag tacgtacagg ccgcacgcga gctgtttgtt cccgaaacaa
    38041 attgcgccga ggtttactat cagttccgcg aagagttcaa caaaagccag gatccgttcc
    38101 gtcgggcggt actgttttta tatttgaacc gctacggtta caacggtctg tgtcgttaca
    38161 atctgcgcgg tgagtttaac gtgccgtttg gccgctacaa aaaaccctat ttcccggaag
    38221 cagagttgta tcacttcgct gaaaaagcgc agaatgcctt tttctactgt gagtcttacg
    38281 ccgatagcat ggcgcgcgca gatgatgcat ccgtcgtcta ttgcgatccg ccttatgcgc
    38341 cgctgtctgc gaccgccaac tttacggcgt atcacacaaa cagttttacg cttgaacaac
    38401 aagcgcatct ggcggagatc gccgaaggtc tggttgatcg ccatattcca gtgctgatct
    38461 ccaatcacga tacgatgtta acgcgtgagt ggtatcagcg cgcaaaattg catgtcgtca
    38521 aagttcgacg cagtataagc agcaacggcg gcacacgtaa aaaggtggac gaactgctgg
    38581 ctttgtacaa accaggagtc gtttcacccg cgaaaaaata attctcaagg agaagcggat
    38641 gaaacagtat ttgattgccc cctcaattct gtcggctgat tttgcccgcc tgggtgaaga
    38701 taccgcaaaa gccctggcag ctggcgctga tgtcgtgcat tttgacgtca tggataacca
    38761 ctatgttccc aatctgacta ttgggccaat ggtgctgaaa tccttgcgta actacggcat
    38821 taccgcccct atcgacgtac acctgatggt gaaacccgtc gatcgcattg tgcctgattt
    38881 cgctgccgct ggtgccagca tcattacctt tcatccagaa gcctccgagc atgttgaccg
    38941 cacgctgcaa ctgattaaag aaaatggctg taaagcgggt ctggtattta acccggcgac
    39001 acctctgagt tatctggatt acgtgatgga taagctggat gtgatcctgc tgatgtccgt
    39061 caatcctggt ttcggcggtc agtctttcat tcctcaaaca ctggataaac tgcgcgaagt
    39121 acgtcgtcgt atcgacgagt ctggctttga cattcgattg gaagtggacg gtggcgtgaa
    39181 ggtgaacaac attggcgaaa tcgctgcggc gggcgcggat atgtttgttg ctggttcggc
    39241 aatcttcgac cagccagact acaaaaaagt cattgatgaa atgcgcagtg aactggcaaa
    39301 ggtaagtcat gaataagttt gaagatattc gcggcgtcgc ttttgatctc gacggtacgc
    39361 tggttgacag tgcacccggt cttgctgctg cggtagatat ggcgctgtat gcgctggagt
    39421 tgcctgtcgc aggtgaagaa cgcgttatta cctggattgg taacggcgca gatattctga
    39481 tggagcgcgc attgacctgg gcgcgtcagg aacgtgcgac tctgcgtaaa acaatgggta
    39541 aaccgcccgt tgatgacgac attccggcag aagaacaggt acgtattctg cgtaaactgt
    39601 tcgatcgcta ctatggcgag gttgccgaag aggggacgtt tttgttcccg cacgttgccg
    39661 atacgctggg cgcattgcag gctaaaggcc tgccgctagg cctggtcacc aacaaaccga
    39721 cgccgttcgt cgcgccgctg ctcgaagcct tagatatcgc caaatacttc agcgtggtta
    39781 tcggcggtga tgatgtgcaa aacaaaaaac cgcatccgga cccgctgtta ctggtggctg
    39841 agcggatggg aattgcccca caacagatgc tgtttgttgg cgactcacgc aatgatattc
    39901 aggcagcaaa agcggcaggt tgcccatcag ttggcttaac ctacggatat aactacggcg
    39961 aggctatcga tctcagccag cctgatgtaa tttatcagtc tataaatgac cttctgcccg
    40021 cattagggct tccgcatagc gaaaatcagg aatcgaaaaa tgactaagcc catcgttttt
    40081 agtggcgcac agccctcagg tgaattgacc attggtaact acatgggtgc gctgcgtcag
    40141 tgggtaaaca tgcaggatga ctatcattgc atttactgta tcgttgacca acacgcgatc
    40201 accgtgcgcc aggatgcaca gaagctgcgt aaagcgacgc tggatacgct ggccttgtat
    40261 ctggcttgtg gtatcgatcc tgagaaaagc accatttttg ttcagtccca cgtgccggaa
    40321 catgcacagt taggctgggc actgaactgc tatacctact tcggcgaact gagccgcatg
    40381 acgcagttta aagataaatc tgcgcgttat gccgagaaca tcaatgctgg tctgtttgac
    40441 tatccggtgc tgatggctgc ggacatcctg ctgtatcaaa ctaatctggt accggtcggt
    40501 gaagaccaga aacagcacct ggaactgagc cgcgatatcg cccagcgttt caacgcgctg
    40561 tatggcgaga tctttaaagt gccggagccg tttattccga aatctggcgc gcgcgtaatg
    40621 tcgctgctgg agccgaccaa aaagatgtcc aagtctgacg ataaccgcaa taacgttatc
    40681 ggcctgctgg aagatccgaa atcggtagtg aagaaaatca aacgtgcggt cactgactcc
    40741 gacgagccgc cggtagttcg ctacgatgtg cagaacaaag cgggcgtttc caacctgctg
    40801 gatatccttt ctgcggtaac gggccagagc atcccggaac tggaaaaaca gttcgaaggc
    40861 aagatgtatg gtcatctgaa aggcgaagtg gctgatgccg tttccggtat gctgactgaa
    40921 ttgcaggaac gctatcaccg tttccgcaat gacgaagcct tcctgcaaca ggtgatgaaa
    40981 gatggcgcgg aaaaagccag tgcacacgct tctcgtacgc tgaaagcggt gtacgaagcg
    41041 attggttttg tggcgaagcc gtaattcact attgctggca aattgcgctt tgttcatgcc
    41101 ggatgcggcg tgaacgcctt atccggccta caaagtaatg cacatattgc aaggaaatgt
    41161 aggcctgata agcgtagcgc atcaggcaat tttgctttta tcatcactct gaaaccgggg
    41221 aaacccggtt tttttatcct catttgcaat aatccgaaaa aatgtgaagt gcctcgccgt
    41281 ttccacatct cgctgttgcg ccttatcttc aaattcctga agatataaat attcaattta
    41341 ctgaaaattt attatgcgtc gaacgtttat caaaaaagaa ggcgtcgtca tcacgacact
    41401 ggcccgttat ttgttgggtg aaaagtgcgg taatcgactg aaaaccatag atgaactggc
    41461 aactgaatgc cgttcatccg ttggcctgac gcaggccgcg ttgaaaacgc tggaatcaag
    41521 cggagcgata cggatagaac gccgtgggcg taatggcagt tatctggtgg aaatggataa
    41581 caaagcattg ctgagtcatg tggatatcaa caatgtggtg tgcgcgatgc ccttgcccta
    41641 tacccgtttg tacgaaggcc tggcgagcgg attgaaagct cagtttgatg gtattccttt
    41701 ttactatgcg cacatgcgcg gcgcggatat tcgcgtggag tgtttactta acggcgtgta
    41761 tgacatggcg gttgtttccc gactggcggc ggaaagttat ctcacgcaaa aagggttatg
    41821 cctcgcgctg gagttggggc cgcacaccta cgttggcgag caccagttga tttgccgtaa
    41881 aggcgagtcc gcaaacgtga atcgcgtggg gctggataac cgttcggcgg atcagaaaat
    41941 catgaccgat gtcttttttg gcgatagtga tgtggaacga gtcgatctct cttatcacga
    42001 gagtttacaa cgcattgtta aaggcgatgt tgatgcggtg atctggaacg tggtggcgga
    42061 aaacgaactg accatgctgg gattagaagc gacgccgctc acagacgatc cgcgattttt
    42121 acaggccacc gaagccgtag ttttgacgcg agtcgatgat tacccaatgc aacaattgct
    42181 gcgtgcagtt gttgataaac aggccctgct tgcccatcaa caacgagtgg tcagtgggga
    42241 acaggaaccg agttattaaa cgaaaggcat ctgaaatgga aaccagactc aacctgcttt
    42301 gtgaggcagg cgttattgat aaggacatct gcaaaggcat gatgcaggtc gtcaacgtac
    42361 tggaaacaga gtgtcatcta ccggtgcgca gcgagcaagg aatgatggcg atgacacata
    42421 tggcgagcgc actgatgcgc agtcgccgtg gtgaagaaat agagccgctg gatgacgaat
    42481 tgctggcaga gctggcgcaa tccagccact ggcaagccgt tgtgcaattg catcaggtgt
    42541 tgttgaagga attcgcactg gaagttaagc cgtgtgaaga aggctatttg ctggctaatc
    42601 tttatggatt atggatggct gctaacgaag aggtttgaag ctcccgacaa atccacgtcg
    42661 cataatctaa atattcagaa aaatgaatat ttaattcaag gaacagaaac gaggcaacaa
    42721 gatgtttgta gaagcattga aacgccagaa cccggcgctg atttccgccg cactaagttt
    42781 gtggcagcag ggcaagatcg ccccggacag ctgggtgatc gatgtggatc agatactgga
    42841 aaacggtaag cggctgattg agacggcgcg actttacggc attgaactgt atctgatgac
    42901 caagcagttt ggccgtaatc cgtggctggc ggaaaaactt ctggcactgg gctacagcgg
    42961 cattgtggcg gtggattaca aagaggcgcg ggtcatgcgc cgcgccggtc tgccgttggc
    43021 gcatcagggg catctggtgc aaatcccttg tcaccaggtt tctgacgccg ttgcacaggg
    43081 aactgacgtc atcaccgtgt ttactctcga taaagcgcgg gaagtttctg cagcagcggt
    43141 gaaggccgga cgtgtgcagt ctgtgctgct caaagtttat agcgacgatg attttcttta
    43201 tccggggcag gagagcggtt ttgtacagca ttcgctgcac gaggtggttg ctgatattaa
    43261 gaaactgccg gggctgcatt tagccggact aacccatttc ccttgcctgc tttgggatga
    43321 ggctgccggg aaagttttgc cgacaccgaa tcttcacacg ctgatacagg cacgggatca
    43381 actggcgaaa tctggtattg cgattgagca actgaacgca ccttctgcga ccagctgcac
    43441 ttcgctgcca ttgctggcgg aatacggtgt gactcatgcc gaacccggtc atgcgctgac
    43501 ggggacgatt ccggcaaacc agcagggcga tcagcctgaa cgtatcgcga tgctctggtt
    43561 aagtgaaatc tcccatcatt ttcgtggcga cagctactgc tacggcggcg gttactaccg
    43621 tcgtggtcac gcgcaacatg cgctggtgtt tacgccagaa aatcaaaaga ttactgaaac
    43681 caatctcaaa actgtggatg acagcagtat cgactacacc ctgccgctgg caggcgagtt
    43741 tccggtaagc agtgcagtag tgctctgttt tcgcacgcag atttttgtca cccgtagcga
    43801 tgtggtgttg ctgtccggta ttcaccgtgg cgaaccagaa atcgtcggtc gttatgacag
    43861 tcttggaaac tctctggggg cgtaatggcg cgatttgtgg tgttagtgat tgatagcttt
    43921 ggcgtagggg caatgaaaga tgtcacgttg gtgcgtccgc aggatgccgg ggcaaatacc
    43981 tgtggtcaca tcctgagcca gttgccgcat ttgcagctac caatgctgga gaaaatgggg
    44041 ctaatcaacg cattaggtta tgcgcctgga gatatgcagc cgtcagactc cgcaacctgg
    44101 ggcgtggcag agctgcaaca tgaaggtggc gataccttta tggggcatca ggaaatttta
    44161 ggcacgcgcc cgttaccgcc gctacggatg ccctttcgcg atgtgattga ccgtgttgag
    44221 caagcattac ttaacgctga ctggcaggtg gagcgccgtg gcgatgatct gcaatttctg
    44281 tgggtcaatc aggcggttgc gattggcgat aatctcgagg cggatttagg tcaggtctat
    44341 aacatcaccg ccaatctctc tgtgatctct tttgacgacg caatcaaaat tggtcgtatc
    44401 gtgcgtgagc aggtacaggt cggtcgggtc attacatttg gtggcctgtt aaccgacagc
    44461 cagcgcatcc tcgatgccgc agaaagcaaa gaagggcgct ttattggcat caatgcgccg
    44521 cgttctggcg cttatgacaa cggcttccag gtcgtgcata tgggctacgg cgtcgatgaa
    44581 aaagtgcagg tgccacaaaa actgtatgaa gtgggcgtac caaccgtgct ggtggggaaa
    44641 gtggcggata ttgccagtaa tccttatggc gtgagctggc aaaacctggt ggatagccag
    44701 cggattatgg atatcacctt caacgaattt aacacccatc cgacggcgtt tatttgtacc
    44761 aatatccagg aaaccgacct cgctggtcat gcagaagacg tcgcacgtta tgcggaacgt
    44821 ttgcaggtcg ttgaccgtaa ccttgcccgg cttattgagg cgatgcagcc agatgattgc
    44881 ctggtcgtga tggcggatca cggtaacgat ccgaccattg gtcacagcca ccatacccgc
    44941 gaagtggtgc cagtgctggt ttatcagcaa gggatggtcg ctacgcagct tggtgtgctc
    45001 accacgcttt ctgatgtggg ggctaccgta tgtgaatttt tccgcgcgcc accgccacaa
    45061 aatggtcgct cttttctttc ctccctccgg tttgcaggag acaccctatg agttttgatc
    45121 cgacgggcta caccctcgcc catgagcatc tgcatattga tctctccggc tttaaaaaca
    45181 acgtggactg ccgccttgat cagtatgcgt tcatttgcca ggagatgaac gacctgatgg
    45241 cccagggcgt gcgtaatgtg attgagatga ccaaccgtta catggggcgc aatgcgcaat
    45301 ttatgctcga tgtaatgcgc gaaaccggga tcaacgtggt ggcctgtacc ggttattacc
    45361 aggacgcgtt tttcccggaa catgtggcga cccgcagcgt gcaggaactg gcgcaagaga
    45421 tggtcgatga aattgaacag ggtatcgatg gcacggagct gaaagccggg atcatcgcgg
    45481 agataggctc cagcgaagga aaaatcacgc cgctggaaga gaaggtattt attgccgccg
    45541 cgctggcgca taaccagacc ggacgcccga tctccacgca tacctctttt agcaccatgg
    45601 ggctggaaca actggcgcta ctgcaagccc acggagttga tctttcgcgc gtcaccgttg
    45661 gtcactgcga tctgaaagac aacctcgaca acattgtgaa gatgatcgat ctcggcgcgt
    45721 atgtgcagtt cgacaccatc ggtaagaaca gttactaccc ggacgaaaag cgcattgcga
    45781 tgctccacgc gctacgtgac cgaggtttgc ttaaccgcgt catgctgtcg atggatatta
    45841 cgcgccgctc ccatttaaaa gccaacggtg gttatggcta tgactattta ttgaccacct
    45901 ttattccgca attgcgccag tcaggattca gtcaggccga tgtggatgtg atgttacgtg
    45961 aaactccctc tcaatttttc caataaggac agactcatga aaaagattgg tgttgcaggc
    46021 ttacagcgtg agcagattaa aaaaaccatt gaagcgacgg ctcctggctg ttttgaagtt
    46081 ttcattcaca acgacatgga agcggcaatg aaagtcaaat ccgggcaact ggattactac
    46141 atcggcgcgt gtaataccgg tgcgggcgcg gcattgtcga ttgccatcgc ggtgattggc
    46201 tataacaaaa gttgcaccat tgccaaacca ggcattaaag cgaaagacga acatatcgcc
    46261 aaaatgatcg ccgaagggaa agtggcgttt gggctttccg ttgagcacgt cgaacacgcg
    46321 attccgatgc tgattaacca tctgaaataa aaggcacgac tatggatctg tatattcaga
    46381 ttatcgtggt ggcgtgcctg acgggtatga catcgcttct ggcgcatcgc tcggcggccg
    46441 tttttcatga cggtatccgc ccgatcctgc cgcaactgat tgaaggctat atgaaccgtc
    46501 gcgaggcggg gagtatcgct tttggtctga gcattggttt tgtggcttcg gtggggatct
    46561 cttttaccct gaaaaccgga ctgctcaacg catggttact ctttcttcct accgatatcc
    46621 tcggcgtact ggcgataaac agcctgatgg cgtttggtct tggcgctatc tggggcgtgt
    46681 tgatccttac ttgcctgtta ccggtaaacc agctgctgac cgcgctaccg gtggatgtat
    46741 taggtagcct gggggaatta agctcgccgg tggtttcagc ttttgcactg ttcccgttgg
    46801 tggcgatttt ctaccagttt ggctggaagc aaagtctgat cgccgccgtg gtggtactga
    46861 tgacccgtgt ggtagtagtg cgctatttcc cgcatctcaa tccggaatcc atcgaaatct
    46921 ttattggcat ggtgatgctg ctggggatcg cgattactca cgacctgcgt catcgtgatg
    46981 aaaatgacat tgatgccagc gggctttcgg tgtttgaaga acgcacgtca cggattatca
    47041 aaaacttacc gtatatcgct attgtcgggg cgctgattgc cgccgttgcc agcatgaaga
    47101 tttttgctgg cagtgaagtg tcgatcttca cactggagaa agcatattcc gcaggcgtaa
    47161 cgccggaaca atcgcaaaca ctaatcaacc aggcagctct ggcggaattt atgcgcgggc
    47221 tgggttttgt accgttgatt gccaccaccg cgttagccac tggtgtgtat gcagttgcgg
    47281 gctttacctt tgtttatgcg gtgggctatc tctcgccgaa tccgatggtt gcagcggtat
    47341 taggcgcagt ggttatttcg gcggaagtct tgctgcttcg ttcgatcggc aaatggttgg
    47401 ggcgctaccc gtcggtacgt aatgcgtcgg ataacatccg taacgccatg aatatgctga
    47461 tggaagtggc gttgctggtc ggttcgatct ttgcagcaat caaaatggcg ggctataccg
    47521 gattctctat cgcggttgcc atttacttcc tcaacgaatc cctgggccgt ccggtacaga
    47581 aaatggcggc accggtcgta gcagtaatga tcaccggcat cctgctgaat gttctttact
    47641 ggcttggcct gttcgttccg gcttaaggaa ggcacctatg aagacgtttc ctctgcaaag
    47701 cctgacgctt attgaggcgc agcaaaagca gtttgcgctg gtggacacga tttgccgtca
    47761 ttttcctggc agcgagtttc ttacctgcgg tgatttgggc ttaacgccgg ggctaaatca
    47821 gccccgcatt acccagcgtg tggaacaggt gctggctgac gcgtttcatg cacccgcggc
    47881 ggcactggta cagggcgcgg gtactggcgc gattcgtgcg gcactggcgg ctttgctcaa
    47941 accggggcaa cgtcttctgg tgcatgatgc gccggtttat ccaacgacgc gcgtcattat
    48001 tgagcagatg gggctaacgc tcattacggc tgatttcaat gatctgtcgg cactgaagca
    48061 ggttgttgac gagcaacaac ctgatgccgc gctggtgcag catacgcgcc agcagccgca
    48121 ggaccgctat gtgctggcag acgtgctggc aacgttgcgc gcggcaggtg ttccagcgtt
    48181 aaccgatgac aactatgcgg tgatgaaggt gtcgcgaatt ggctgcgaat gcggggcgaa
    48241 tgtgtcgaca ttttcctgct tcaagctatt tgggccagag ggcgttggtg cggtggtagg
    48301 cgatgccgat gttattagcc ggattcgcgc cacgctctat tccggcggca gtcagattca
    48361 gggggcgcag gcattagagg tgttgcgtgg tctggtgctc gcgccagtga tgcacgcggt
    48421 gcaggcgggg gtatctgaac ggttgctggc tttacttaac ggtggcgcag tggcggaagt
    48481 gaaaagcgcg gtgattgcca atgcgcagtc gaaggtgttg attgtggagt ttcatcagcc
    48541 gattgccgcc agagtactgg aagaggcgca aaagcgcggt gccttgcctt acccggttgg
    48601 tgcagagtcg aaatatgaaa ttccgccact tttttatcga ctttccggaa cgtttcgcca
    48661 ggcgaatccg caactcgaac attacgcgat tcgtattaac ccaaatcgca gcggtgaaga
    48721 gacggtgctg cggattttgc gtgagagtat tgccagtatt taatcttgtg catgatgttt
    48781 tcgaaaagaa tgcagaaata aatacgccct cgggtaaccc cgagggcgtc aggcaattaa
    48841 accggtgtta gccgctttca gtcagagact tactgtgcag taggaccgca accgccaatg
    48901 attttagaaa tagaaattgc cgggtgcagc aggtagtcgt aagagcagtt cttatcacgg
    48961 ttttcaatat gaccagtaca tgcagttaac gtagccagta ccgcacctac cagtgcaact
    49021 ttaataaatt tgttcattt
//
LOCUS       DAZKGF010000013        47626 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZKGF010000013 DAZKGF010000000
VERSION     DAZKGF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.013"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..313)
                     /locus_tag="ACHOQV_000755"
     CDS             complement(<1..313)
                     /locus_tag="ACHOQV_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9629172.1"
                     /translation="MNNPNPCMTCGACCAFFRVSFYWAEADDAGGKVPVSLTEQISPF
                     HRCMRGTSQKNPRCVALAGTPGKNAYCSVYKNRPSTCREFAMSGENGIANEACNRARA
                     KY"
     gene            complement(453..540)
                     /locus_tag="ACHOQV_000756"
     tRNA            complement(453..540)
                     /locus_tag="ACHOQV_000756"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(504..506),aa:Ser,seq:tga)
     gene            967..2085
                     /gene="hyaA"
                     /locus_tag="ACHOQV_000757"
     CDS             967..2085
                     /gene="hyaA"
                     /locus_tag="ACHOQV_000757"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFP9629173.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     KMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2082..3875
                     /gene="hyaB"
                     /locus_tag="ACHOQV_000758"
     CDS             2082..3875
                     /gene="hyaB"
                     /locus_tag="ACHOQV_000758"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFP9629174.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLSDDGSELISVQVR"
     gene            3894..4600
                     /gene="hyaC"
                     /locus_tag="ACHOQV_000759"
                     /pseudo
     CDS             3894..4600
                     /gene="hyaC"
                     /locus_tag="ACHOQV_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            4597..5184
                     /gene="hyaD"
                     /locus_tag="ACHOQV_000760"
     CDS             4597..5184
                     /gene="hyaD"
                     /locus_tag="ACHOQV_000760"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFP9629175.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPAKESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            5181..5579
                     /gene="hyaE"
                     /locus_tag="ACHOQV_000761"
     CDS             5181..5579
                     /gene="hyaE"
                     /locus_tag="ACHOQV_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFP9629176.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGS
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            5576..6433
                     /gene="hyaF"
                     /locus_tag="ACHOQV_000762"
     CDS             5576..6433
                     /gene="hyaF"
                     /locus_tag="ACHOQV_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFP9629177.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQQQISERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            6567..8111
                     /gene="appC"
                     /locus_tag="ACHOQV_000763"
     CDS             6567..8111
                     /gene="appC"
                     /locus_tag="ACHOQV_000763"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFP9629178.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8123..9259
                     /gene="appB"
                     /locus_tag="ACHOQV_000764"
     CDS             8123..9259
                     /gene="appB"
                     /locus_tag="ACHOQV_000764"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP9629179.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            9272..9364
                     /gene="cbdX"
                     /locus_tag="ACHOQV_000765"
     CDS             9272..9364
                     /gene="cbdX"
                     /locus_tag="ACHOQV_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFP9629180.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            9444..10742
                     /gene="appA"
                     /locus_tag="ACHOQV_000766"
     CDS             9444..10742
                     /gene="appA"
                     /locus_tag="ACHOQV_000766"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFP9629181.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQCLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFSQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(10862..13042)
                     /gene="etk"
                     /locus_tag="ACHOQV_000767"
     CDS             complement(10862..13042)
                     /gene="etk"
                     /locus_tag="ACHOQV_000767"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFP9629182.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIVSVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEGFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13062..13508)
                     /gene="etp"
                     /locus_tag="ACHOQV_000768"
                     /gene_synonym="yccY"
     CDS             complement(13062..13508)
                     /gene="etp"
                     /locus_tag="ACHOQV_000768"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFP9629183.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(13496..14635)
                     /gene="gfcE"
                     /locus_tag="ACHOQV_000769"
     CDS             complement(13496..14635)
                     /gene="gfcE"
                     /locus_tag="ACHOQV_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFP9629184.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(14681..16777)
                     /gene="gfcD"
                     /locus_tag="ACHOQV_000770"
     CDS             complement(14681..16777)
                     /gene="gfcD"
                     /locus_tag="ACHOQV_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP9629185.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(16777..17523)
                     /gene="gfcC"
                     /locus_tag="ACHOQV_000771"
     CDS             complement(16777..17523)
                     /gene="gfcC"
                     /locus_tag="ACHOQV_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP9629186.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVVATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWLAGRSVTDYLQDHTRLAGADKNNVMVITPEGEAVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(17520..18164)
                     /gene="gfcB"
                     /locus_tag="ACHOQV_000772"
     CDS             complement(17520..18164)
                     /gene="gfcB"
                     /locus_tag="ACHOQV_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFP9629187.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(18271..18576)
                     /locus_tag="ACHOQV_000773"
     CDS             complement(18271..18576)
                     /locus_tag="ACHOQV_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629188.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19017..19229)
                     /gene="cspH"
                     /locus_tag="ACHOQV_000774"
     CDS             complement(19017..19229)
                     /gene="cspH"
                     /locus_tag="ACHOQV_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFP9629189.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            19515..19727
                     /gene="cspG"
                     /locus_tag="ACHOQV_000775"
     CDS             19515..19727
                     /gene="cspG"
                     /locus_tag="ACHOQV_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFP9629190.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            19738..19926
                     /gene="ymcF"
                     /locus_tag="ACHOQV_000776"
     CDS             19738..19926
                     /gene="ymcF"
                     /locus_tag="ACHOQV_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9629191.1"
                     /translation="MTPHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            19901..20131
                     /gene="ymcE"
                     /locus_tag="ACHOQV_000777"
     CDS             19901..20131
                     /gene="ymcE"
                     /locus_tag="ACHOQV_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFP9629192.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            20121..20294
                     /gene="gnsA"
                     /locus_tag="ACHOQV_000778"
     CDS             20121..20294
                     /gene="gnsA"
                     /locus_tag="ACHOQV_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP9629193.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(20342..21415)
                     /gene="yccM"
                     /locus_tag="ACHOQV_000779"
     CDS             complement(20342..21415)
                     /gene="yccM"
                     /locus_tag="ACHOQV_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9629194.1"
                     /translation="MTEKRRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVTRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDTPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLNFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLILGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(21487..24231)
                     /gene="torS"
                     /locus_tag="ACHOQV_000780"
     CDS             complement(21487..24231)
                     /gene="torS"
                     /locus_tag="ACHOQV_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFP9629195.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     DFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKANQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPQRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLLLRIATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAQALETLQNSEPFAVALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDDIDIARANQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            24314..25342
                     /gene="torT"
                     /locus_tag="ACHOQV_000781"
     CDS             24314..25342
                     /gene="torT"
                     /locus_tag="ACHOQV_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFP9629196.1"
                     /translation="MRVLLLLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGNPLNVLLMPGPDNAGGSKEMAEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQEQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(25315..26007)
                     /gene="torR"
                     /locus_tag="ACHOQV_000782"
     CDS             complement(25315..26007)
                     /gene="torR"
                     /locus_tag="ACHOQV_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFP9629197.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            26137..27309
                     /gene="torC"
                     /locus_tag="ACHOQV_000783"
     CDS             26137..27309
                     /gene="torC"
                     /locus_tag="ACHOQV_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFP9629198.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            27309..29855
                     /gene="torA"
                     /locus_tag="ACHOQV_000784"
     CDS             27309..29855
                     /gene="torA"
                     /locus_tag="ACHOQV_000784"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFP9629199.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKEGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGINAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            29852..30451
                     /gene="torD"
                     /locus_tag="ACHOQV_000785"
     CDS             29852..30451
                     /gene="torD"
                     /locus_tag="ACHOQV_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFP9629200.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIATQTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDNLRQKTLTA
                     LRQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(30706..31011)
                     /gene="cbpM"
                     /locus_tag="ACHOQV_000786"
     CDS             complement(30706..31011)
                     /gene="cbpM"
                     /locus_tag="ACHOQV_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFP9629201.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(31011..31931)
                     /gene="cbpA"
                     /locus_tag="ACHOQV_000787"
     CDS             complement(31011..31931)
                     /gene="cbpA"
                     /locus_tag="ACHOQV_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFP9629202.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            32084..32185
                     /locus_tag="ACHOQV_000788"
                     /pseudo
     CDS             32084..32185
                     /locus_tag="ACHOQV_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32466..33707
                     /gene="agp"
                     /locus_tag="ACHOQV_000789"
     CDS             32466..33707
                     /gene="agp"
                     /locus_tag="ACHOQV_000789"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFP9629203.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
     gene            complement(33745..33972)
                     /gene="yccJ"
                     /locus_tag="ACHOQV_000790"
     CDS             complement(33745..33972)
                     /gene="yccJ"
                     /locus_tag="ACHOQV_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFP9629204.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(33993..34589)
                     /gene="wrbA"
                     /locus_tag="ACHOQV_000791"
     CDS             complement(33993..34589)
                     /gene="wrbA"
                     /locus_tag="ACHOQV_000791"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP9629205.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNS"
     gene            34962..35135
                     /gene="ymdF"
                     /locus_tag="ACHOQV_000792"
     CDS             34962..35135
                     /gene="ymdF"
                     /locus_tag="ACHOQV_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP9629206.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(35218..36546)
                     /gene="rutG"
                     /locus_tag="ACHOQV_000793"
     CDS             complement(35218..36546)
                     /gene="rutG"
                     /locus_tag="ACHOQV_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFP9629207.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGHVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(36567..37061)
                     /gene="rutF"
                     /locus_tag="ACHOQV_000794"
     CDS             complement(36567..37061)
                     /gene="rutF"
                     /locus_tag="ACHOQV_000794"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFP9629208.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(37072..37662)
                     /gene="rutE"
                     /locus_tag="ACHOQV_000795"
     CDS             complement(37072..37662)
                     /gene="rutE"
                     /locus_tag="ACHOQV_000795"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFP9629209.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(37672..38472)
                     /gene="rutD"
                     /locus_tag="ACHOQV_000796"
     CDS             complement(37672..38472)
                     /gene="rutD"
                     /locus_tag="ACHOQV_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFP9629210.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLVQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(38480..38866)
                     /gene="rutC"
                     /locus_tag="ACHOQV_000797"
     CDS             complement(38480..38866)
                     /gene="rutC"
                     /locus_tag="ACHOQV_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFP9629211.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(38878..39570)
                     /gene="rutB"
                     /locus_tag="ACHOQV_000798"
     CDS             complement(38878..39570)
                     /gene="rutB"
                     /locus_tag="ACHOQV_000798"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFP9629212.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELMPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            complement(39570..40661)
                     /gene="rutA"
                     /locus_tag="ACHOQV_000799"
     CDS             complement(39570..40661)
                     /gene="rutA"
                     /locus_tag="ACHOQV_000799"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFP9629213.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDSLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAAHAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSDTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIENFGERIQPLMQCRAHLPALTQEVA"
     gene            40949..41587
                     /gene="rutR"
                     /locus_tag="ACHOQV_000800"
     CDS             40949..41587
                     /gene="rutR"
                     /locus_tag="ACHOQV_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFP9629214.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(41627..45589)
                     /gene="putA"
                     /locus_tag="ACHOQV_000801"
     CDS             complement(41627..45589)
                     /gene="putA"
                     /locus_tag="ACHOQV_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9629215.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPPVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGDGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPALIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            45644..45853
                     /locus_tag="ACHOQV_000802"
     CDS             45644..45853
                     /locus_tag="ACHOQV_000802"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629216.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            46012..47520
                     /gene="putP"
                     /locus_tag="ACHOQV_000803"
     CDS             46012..47520
                     /gene="putP"
                     /locus_tag="ACHOQV_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFP9629217.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
BASE COUNT        11237 a        12362 c        12649 g        11378 t
ORIGIN      
        1 cgtattttgc ccgtgcgcgg ttacatgctt cattcgcgat gccattttcg ccggacattg
       61 cgaattcccg gcatgtggac ggacggttct tgtaaacaga gcaatatgcg tttttacccg
      121 gagttcccgc cagagcgaca catcggggat ttttctgact ggtgccgcgc atacagcgat
      181 gaaaggggga tatttgctca gtgagactga ccggaacctt tccgccagca tcgtcagctt
      241 ctgcccagta aaaagagacg cggaaaaatg cacaacaggc accacacgtc atgcatggat
      301 tggggttatt cataattcat cggtatagcg ataaatcgta aagagaacaa tgtacctagt
      361 gtgtattgag ctaaatatac caattagtat accaagtgta tactaagagc aaaaaaagaa
      421 aggggctacc atttggtaac cccttgattg tttggcggaa gcgcagagat tcgaactctg
      481 gaaccctttc gggtcgccgg ttttcaagac cggtgccttc aaccgctcgg ccacacttcc
      541 ggaatgacgc gcactataaa catcccgatg cggcgtgtaa acccctaatt tgtttgtttg
      601 cctgaaaaac agccaaaagt gcattgatag catgaaataa cagcagattg atcatttcat
      661 caccatgaat tccttctctt ttactcgttt agcaaccggc taaacatccc cactgcccgg
      721 caaaaagaaa aataggtcca tttttatcgc taaaagataa atccacacag tttgtattgt
      781 tttgtgcaaa agtttcacta cgctttatta acattacttt ctggcgacgt gcgccagtgc
      841 agaaggatga gctttcgttt tcagcatctc acgtgaagcg atggtttgcc ttgctacagg
      901 gacgtcgctt gccgaccata agcgcccggt gtcctgccgg tgtcgcaagg agaagagacg
      961 tgcgatatga ataacgagga aacattttac caggccatgc ggcgtcaggg cgttacccgg
     1021 cgcagctttc tcaaatattg tagtctggct gccacgtcgc tgggattagg cgcgggaatg
     1081 gcaccaaaga ttgcctgggc gctggagaac aaaccgcgca ttccggtggt atggatccac
     1141 ggtctggaat gcacctgctg taccgaatct tttatccgct ccgctcaccc actggcgaag
     1201 gacgtcatcc tttccctgat ttccctcgat tatgacgata ctttgatggc tgccgccgga
     1261 acccaggcgg aagaagtctt tgaagacatc atcacgcaat acaatggcaa atatatcctc
     1321 gcagtagaag gtaatccgcc gctgggcgag caggggatgt tctgtatcag cagcggacga
     1381 ccgtttattg agaaactcaa acgtgccgct gccggagcca gtgcgattat cgcctgggga
     1441 acctgcgcgt cctggggctg cgtgcaggcc gcgcgaccta atccgacgca ggcaacgcct
     1501 atcgacaaag tcatcaccga caaacccatt atcaaagtac ctggctgccc gccgatcccg
     1561 gatgtgatga gcgccatcat tacttacatg gtgacctttg atcgcttgcc agatgtcgac
     1621 aaaatgggcc gtccactgat gttttatggt cagcgtatcc acgataaatg ttatcgccgc
     1681 gctcacttcg acgccggaga gttcgtccag agttgggatg atgacgctgc ccgcaaaggt
     1741 tactgcctgt acaaaatggg ctgcaaaggg cctaccacct ataacgcctg ttcctccaca
     1801 cgctggaacg atggcgtttc cttcccgatc cagtcaggtc acggctgcct gggttgtgcg
     1861 gaaaatggtt tctgggatcg cggttcgttc tacagccgcg tggtcgatat tccgcaaatg
     1921 ggtacccatt ccaccgccga taccgtcggt ttaaccgcac ttggcgtggt ggcagcggct
     1981 gttggtgtgc acgcagtcgc cagcgccgtt gaccagcgca gacgccataa ccagcaacct
     2041 acagaaaccg aacatcagcc aggcaatgag gataaacagg catgagcact cagtacgaaa
     2101 ctcagggata caccatcaat aatgccggac gccgcctggt ggtcgacccg attacgcgca
     2161 ttgaaggcca catgcgctgc gaagtgaata ttaacgatca gaatgtgatc accaatgccg
     2221 tctcctgcgg caccatgttt cgtgggctgg agatcatcct gcaagggcgc gacccgcgcg
     2281 atgcgtgggc gttcgttgaa cgtatctgcg gcgtctgtac tggcgtacac gctctggctt
     2341 cggtttacgc catcgaagat gccatcggta ttaaagtgcc ggacaacgcc aatatcatcc
     2401 gcaacattat gctggcaacg ctctggtgcc acgatcatct ggtgcacttc tatcagcttg
     2461 ccgggatgga ctggatcgat gtgttagatg cgctgaaagc cgacccgcgg aaaacctccg
     2521 aactggcgca aagcctctcc tcatggccga aatcatcccc tggctatttc ttcgacgtac
     2581 aaaaccgcct gaagaaattc gttgaaggcg ggcagttggg gatcttccgc aatggctact
     2641 gggggcaccc gcagtacaaa ctgccgccag aagccaacct gatgggcttt gcccactatc
     2701 ttgaagctct cgatttccaa cgtgaaattg tcaaaatcca cgcggtcttt ggcggtaaaa
     2761 acccgcatcc aaactggatt gtcggcggga tgccttgtgc cattaacatt gacgaaagcg
     2821 gcgcggtcgg ggcggtcaat atggaacgcc tgaacctggt gcagtcaatc attacccgta
     2881 cggcggattt cattaacaac gtgatgatcc ccgacgcctt agccatcggt cagttcaaca
     2941 agccgtggag cgaaatcggc actggccttt cggataaatg cgttctcagt tacggcgcgt
     3001 tcccggacat cgccaacgac tttggcgaga aaagtctact gatgcctggc ggcgcggtga
     3061 ttaacggcga cttcaacaat gtgctgccag tggatttggt tgatccgcag caggtgcagg
     3121 agtttgtcga tcacgcctgg tatcgttatc ccaacgatca ggtcgggcgt catccgttcg
     3181 atggcattac cgacccgtgg tacaaccccg gcgatgtcaa aggcagcgat accaacattc
     3241 agcagctgaa tgaacaggaa cgctactcgt ggatcaaagc gccacgctgg cgcggtaatg
     3301 cgatggaagt ggggccgctg gcacgcacgt taatcgctta tcacaaaggc gatgcggcga
     3361 ccgttgagtc ggtcgatcgc atgatgtcgg cgttgaacct gccgctttcc ggcatccagt
     3421 caacattagg ccgcattttg tgtcgcgcgc acgaagcgca gtgggccgca ggcaagttgc
     3481 aatatttctt cgacaagctg atgaccaatc tcaaaaacgg caatctcgcc accgcctcca
     3541 ctgagaagtg ggaaccagca acctggccga cagagtgccg tggtgtcggc tttaccgaag
     3601 cgccgcgcgg agcgttaggc cactgggccg ccattcgcga tggcaagatt gatctctacc
     3661 agtgcgtggt gccgaccacc tggaacgcca gcccgcgcga tcctaaaggg caaattggcg
     3721 cttatgaagc ggcgctgatg aacaccaaaa tggcgatccc cgagcaaccg ctggagatcc
     3781 tgcgtactct gcacagcttt gatccgtgcc tcgcctgttc aacacacgtg ctgagcgacg
     3841 acggtagcga gctgatctcc gtgcaggtgc gttaacagcg aaggagaatc atcatgcaac
     3901 agaaaagcga caacgttgtc agccactatg tctttgaagc gccagtgcgt atctggcact
     3961 ggctgacggt gttatgcatg gcggtgttga tggtcaccgg ttactttatc ggcaagccgc
     4021 taccttccgt cagcggcgag gcgacgtatc tgttctatat gggctacatc aggttaattc
     4081 acttcagcgc cgggatggtt tttaccgtgg tcttgctgat gcggatctac tgggcttttg
     4141 tcggcaatcg ctactcacgc gagctgttta tcgtgccagt atggcgtaaa agctggtggc
     4201 agggcgtgtg gtatgaaatc cgctggtatc tgtttctggc aaaacgtccg agtgccgata
     4261 taggtcataa ccccatcgcc caggcggcga tgttcggcta tttcctgatg tcagttttta
     4321 tgatcatcac ggctttgcgc tgtacagcga acacagccag tacgctattt ttgcgccgtt
     4381 ccgttatgtg gtggaatttt tctactggac gggcggcaac tcaatggacg ttcacagctg
     4441 gcatcggctg gggatgtggc tgattggcgc gtttgtgatc ggtcatgtct acatggcgct
     4501 gcgtgaagat atcatgtccg acgacacggt gatctccacc atggtcaacg gctaccgtag
     4561 ccacaaattt ggcaaaataa gtaacaagga gcgttcatga gcgagcaacg cgtggtggta
     4621 atggggctgg gcaacctgct gtgggccgat gaaggcttcg gcgtgcgggt ggcggaacgg
     4681 ctgtatgccc attaccactg gcccgagtat gtggagattg tcgatggcgg tactcaggga
     4741 ctgaacttgc tggggtatgt cgaaagcgcc agtcacttgt tgattctcga tgccattgac
     4801 tacgggctgg aaccaggaac gctgcgaacc tatgccggag aacgcattcc ggcttatctc
     4861 agcgcgaaga aaatgagcct gcaccagaac agcttctccg aagtgctggc gctggcggat
     4921 atacgtggtc atttgccagc acatattgcc ctcgtcggtc tgcaacccgc aatgctcgac
     4981 gactacggcg gtagcctgag cgaactggcg cgggagcaac tgcccgctgc ggaacaatcg
     5041 gcgctggcgc agctggctgc gtggggaatt gtgccgcaac cggctaagga atcgcgctgt
     5101 ctcaattatg actgtctgtc gatggaaaat tacgaaggcg ttcgcttgcg tcagtacagg
     5161 atgaggctgg aggaacaggg atgagcaacg acacaccatt taatgcgttg tggcaacgaa
     5221 tgctggcgcg cggctggacg ccggtcagtg aatgccgtct tgacgactgg cttacgcaag
     5281 cgccagacgg cgtggtgtta ttaagtagtg acccgaaacg cacgccagaa gtcagcgata
     5341 acccggtaat gattggcgaa ttactgcgcg agtttcccga ctatacatgg caggtagcga
     5401 ttgctgatct tgagcagagc gaagccatcg gtgatcgttt tggcgtattt cgttttcccg
     5461 ccacactggt gtttaccggt ggtagctatc gcggcgtgct gaatggtatt cacccgtggg
     5521 cggaactgat aaacctgatg cgtgggcttg tcgaaccaca acaggagcgt gcctcatgag
     5581 cgaaactttt ttccatctgt tggggccagg aacgcaaccg aacgatgaca gtttcagcat
     5641 gaatccactg ccgatcacct gtcaggtgaa tgatgaaccg agtatggcgg ccctggagca
     5701 atgtgctcac agcccgcagg tgattgcgct gttaaacgag ttacaacaac aaataagcga
     5761 acgccagcca ccgttgggcg aggtgctggc agtcgatctg ttaaatctca acgccgacga
     5821 tcgtcacttt atcaatacgc ttctcgggga aggggaagtg tcagtgcgca tacagcaggc
     5881 tgacgacagt gaaagtgaaa tacaggaggc gatcttctgc ggattatggc gggtgcgcag
     5941 acgtcgcggc gacaagttgc tggaggacaa actggaggct ggctgcgcgc cgctggcatt
     6001 gtggcaggcg gcaacgcaaa acgtcttgcc gacagattcg ctgttaccgc cgcccattga
     6061 tggcctgatg aatggcctac cgttggcgca tgagctactg gcgcatgtac gtaaccccga
     6121 cgcgcagccg cacagcatta atctgacgca attacccatc agcgaggctg atcggctttt
     6181 tctctcacgt ctctgtgggc cgggaaatat tcagattcgc accattggct atggcgagag
     6241 ctatatcaac gccacggggt tacgccatgt ctggcattta cgctgcacgg acaccttaaa
     6301 aggcccgtta ctggaaagtt atgaaatctg cccaataccg gaagtggtgc tggcagcgcc
     6361 agaagatttg gtcgactctg cgcagcggct tagcgaggta tgccagtggc tggcggaagg
     6421 tgcaccgacg taaaaagacg gtaagtatcg ctttcaggct tatgaatatc gcaatcggcg
     6481 aatacctctg gtcgtagagt ttcaggataa agagggagat ctaccattaa cgggttattt
     6541 ttctctcttc gcctacagga gtgcgcatgt gggatgtcat tgatttatcg cgctggcagt
     6601 ttgctctgac cgcgctgtat cactttttat ttgtacccct taccctgggg ctgatttttt
     6661 tgctggctat catggaaacc atttacgtgg tcaccggcaa aacaatctac cgcgatatga
     6721 cgcgcttctg gggtaagctc ttcggtatca actttgctct tggcgtggct accggcctga
     6781 cgatggagtt tcagtttggt actaactggt cattctattc caactatgtg ggcgatattt
     6841 ttggcgcacc gctggcgatg gaagcattaa tggccttctt cctcgaatcc acctttgtcg
     6901 ggctgttctt cttcggctgg cagcggctga ataaatacca gcacctgctg gtgacgtggc
     6961 tggtggcgtt cggttcaaat ctctctgcat tgtggatatt gaatgccaac ggttggatgc
     7021 aatacccgac cggtgcgcat tttgatatcg acaccctgcg tatggagatg accagtttca
     7081 gcgagttggt ctttaacccg gtcagccagg tgaaatttgt gcacactgta atggcgggct
     7141 acgtgaccgg ggccatgttt attatggcga tcagcgcctg gtatttactg cgcggacggg
     7201 agcgcgatgt tgcattacgc tcgtttgcca tcggttccgt cttcggtact ctggcgatta
     7261 tcggcacgct gcaactcgga gacagttctg cgtatgaagt cgcgcaagta caaccggtaa
     7321 aactggcggc gatggaaggg gagtggcaaa cggaacctgc acctgcaccg ttccatgtgg
     7381 ttgcctggcc ggaacaggat caagagcgta acgcctttgc tatcaaaatt cccgcgctgc
     7441 tagggatcct cgccactcac tcattagata aacccgtgcc gggtctgaag aatttgatgg
     7501 ctgaaaccta cccacgcttg caacgcggac gtatggcctg gctgttaatg caggaaatat
     7561 cgcaaggcaa tcgtgagccg catgtgttgc aggcatttcg ggagctggaa ggcgaccttg
     7621 gttacggcat gttgctctcc cgctatgcgc cggatatgaa ccatgtcaca gctgcacagt
     7681 accaggcggc gatgcgtggc gcgatacctc aggttgcgcc ggtattctgg agtttccgca
     7741 tcatggtggg ctgtggttct ctgctgctac tggtgatgct gattgcgctt gtccagacgc
     7801 tgcgtggcaa aattgaccag catcgctggg tgctgaaaat ggcgctctgg agtttgccgc
     7861 tgccgtggat tgcgattgaa gccgggtggt ttatgaccga gtttggtcgt cagccgtggg
     7921 cgatacagga catcttaccg acatactccg cgcactccgc cttaaccaca ggacaactgg
     7981 ctttctcact gatcatgatc gtagggcttt acaccctgtt cttaatcgcc gaagtctacc
     8041 tgatgcagaa atatgcccgt ctggggccga gcgcgatgca gagtgaacaa ccgacgcagc
     8101 aacaggggta aaggagaaaa tcatgtttga ttatgaaaca ttgcgcttca tctggtggct
     8161 gctgattggc gtgatcctgg tggtctttat gatctccgac ggatttgaca tggggatcgg
     8221 ctgtctgctg ccgctggtgg cgcgtaatga tgatgaacgt cggatagtga taaacagcgt
     8281 tggcgcgcac tgggaaggca atcaggtctg gttgatcctc gcaggtgggg cattatttgc
     8341 cgcctggccc agagtgtatg cagcggcgtt ttccggcttt tatgtggcga tgattctggt
     8401 gctgtgctcg ctgttcttcc gcccgctggc ctttgattat cgcggaaaaa tcgccgatgc
     8461 acgctggcgt aaaatgtggg atgccggact ggtcatcggc agtctggtgc cgccggtagt
     8521 cttcggtatt gcattcggca acttgctgct cggcgtgccg tttgctttca caccgcaatt
     8581 acgcgtggaa tatctcggca gcttctggca actgctgacg ccattccctt tattgtgtgg
     8641 attgctcagt cttgggatgg tgattttgca aggtggcgtc tggttacaac tgaaaactgt
     8701 tggtgtgatt catctgcgtt cacagctggc gaccaaacgc gccgcactac tggtgatgct
     8761 gtgctttttg ctggcgggtt actggctgtg ggttggtatt gatggctttg tactgctcgc
     8821 ccaggatgct aacggtcctt ccaatccgtt aatgaaactg gtggcagtgc tacctggtgc
     8881 ctggatgaat aatttcgtcg aatcgcccgt tttgtggatc ttcccgctgc tggggttctt
     8941 ctgcccattg ctgacggtga tggcgattta tcgtggtcgc ccgggttggg gatttttgat
     9001 ggcgtcattg atgcaatttg gcgtgatttt cacggcaggc atcacgctgt tcccctttgt
     9061 catgccgtca agcgtgagtc cgatctccag cctgacgttg tgggacagca cttccagtca
     9121 gctgacgctg agcattatgt tggtaatcgt gctgatattt ttgcctattg tgttgctcta
     9181 cactctctgg agctactaca aaatgtgggg acgcatgacg acagaaactc tccgccgtaa
     9241 cgaaaacgag ctgtactaag gagcaggaac aatgtggtat ttactttggt tcgtcggcat
     9301 tttgttgatg tgttcgctct ccacccttgt gttggtatgg ctggacccgc gtctgaaaag
     9361 ttaacgaacg taagcctgat gtggtgcatt agcatcgcat caggcaatca ataatgtcag
     9421 atatgaaaag cggaaacata tcgatgaaag cgatcttaat cccattttta tctcttctga
     9481 ttccgttaac cccgcaatct gcattcgctc agagtgagcc ggagctgaag ctggaaagtg
     9541 tggtgattgt cagtcgtcat ggtgtgcgtg ctccaaccaa ggccacgcaa ctgatgcagg
     9601 atgtcacccc agacgcatgg ccaacctggc cggtaaaact gggttggctg acaccgcgcg
     9661 gtggtgagct aatcgcttat cttggacatt accaacgcca gtgtctggtg gctgacggat
     9721 tgctggcgaa aaagggctgc ccgcagcctg gtcaggtcgc gattattgct gatgtcgacg
     9781 agcgtacccg taaaacaggc gaagccttcg ccgccgggct ggcacctgac tgtgcaataa
     9841 ccgtacatac ccaggcagat acgtccagtc ccgatccgtt atttaatccc ctaaaaactg
     9901 gcgtttgcca actggataat gcgaacgtga ctgacgcgat cctcagcagg gcaggagggt
     9961 caattgctga ctttaccggg catcggcaaa cggcgtttcg cgaactggaa cgggtgctta
    10021 atttttcgca atcaaacttg tgccttaacc gtgagaaaca ggacgaaagc tgttcattaa
    10081 cgcaggcatt accatcggaa ctcaaggtga gcgccgacaa tgtctcatta accggtgcgg
    10141 taagcctcgc atcaatgctg acggagatat ttctcctgca acaggcacaa ggaatgccgg
    10201 agccggggtg gggaaggatc actgattcac accagtggaa caccttgcta agtttgcata
    10261 acgcgcaatt ttatttacta caacgcacgc cagaggttgc ccgcagtcgc gccaccccgt
    10321 tattggattt gatcatggca gcgttgacgc cccatccacc gcaaaaacag gcgtatggtg
    10381 tgacattacc cacttcagtg ctgtttattg ccggacacga tactaatctg gcaaatctcg
    10441 gcggcgcact ggagctcaac tggacgcttc caggtcagcc ggataacaca ccgccaggtg
    10501 gtgaactggt gtttgaacgc tggcgtcggc taagcgataa cagccagtgg attcaggttt
    10561 cgctggtctt ccagacttta cagcagatgc gtgataaaac gccgctgtca ttaaatacgc
    10621 cgcccggaga ggtgaaactg accctggcag gatgtgaaga gcgaaatgcg cagggcatgt
    10681 gttcgttggc aggttttacg caaatcgtga atgaagcacg cataccggcg tgcagtttgt
    10741 aatgcataaa aaagagcatt cagttatctg aatgctctga ggctgatgac aaacgaagaa
    10801 ctgtctaatg cgtagaccgg aaaaggcgtt cacgccccat ccggccacct taccttcagt
    10861 tttactcttt ctcggagtaa ctataaccgt aatagttata gccgtaactg taagcggtgc
    10921 tggcgcgttt aatcacacca ttgaggattg cgcctttaat attaacgcca gcctgttcca
    10981 gacgctgcat tgacaagctc acctctttgg cggtgttcaa gccaaaacgc gcaaccagca
    11041 ggctggtgcc gacagagcgt cccacgaccg cagcatcgct caccgccagc atcggcggcg
    11101 tatcgacaat caccagatcg taatggtcgt tcgcccattc cagtaattga cgcatccgat
    11161 cgcgcatcag cagttcagac gggttaggtg gcacctgacc gcgagtaatc acatcaaagc
    11221 ctcctttgcc aaaatgctgg atcactttgt tgagctcatc tttacctgcc agatattccg
    11281 acaagccatg ttcattactc acggtaaaca ggttatgcga ataaccacgg cgtaagtcgg
    11341 catcaataaa taacactttt tgatcggact gggcgatcac cgctgccaga gttgaactga
    11401 cgaacgtttt accactgtct ggcgtcgcac cggtgatcat cagaatgtta ttctctgtct
    11461 ccatcatagc gaagtgcaga ctggttcgta gcgcacgtac ggcctccaca gcagaatccg
    11521 ccgggttatc caccgccagg aaggggatat ttttagtacg atggcgctgc tgattagaaa
    11581 ataaattttt cttacgcaga cgggtacgtt tatccagcca ctcggacatt gggatagtgg
    11641 cataaacgct gatgccgtgc tcttccagtt gttccggggc ttctacacca cgacgcaaca
    11701 tcgcacgcgc cagcacggca cccaccgaaa taaacaggcc aagaataaaa ccaagcacca
    11761 cattcaacgc ttttttcggt ttcactggct gcggctgagt gactgccggg tcgataatcc
    11821 gcacgttacc aatcgcactg gatttcgaaa tactcaactc ctgctggcgg ttaagtaatt
    11881 gcagatatac cgcacggccc gcttctacgt cacgacttaa acgcaacact tcctgttggg
    11941 tggaaggcat tgccgatacc cgcttattca ggcgtttgcg ttcttgctcc agtgtctggc
    12001 gtttttccag cagcgcacga taagttgggt gatctttctt atacagctgg gagatctctg
    12061 cctcgcggaa agtcagctca ttgagttgat tatcaacgtt cacaatctgc tcaagaacgg
    12121 ctttggcttc caggttaagg tcaaccgaat cgcgctgttg gcgataaacg ttgagttttt
    12181 cttccgcctg gtccagctcg ctgcgcactt ctggcaactg gcgctgtaag aattcaaggc
    12241 tttgtgaatc ctgcgccgcc tggcgagcga tattctgttg caaatagttg ttagcgatgc
    12301 tgttcagaat acgagtaatc aactggggat catcaccggt catggtaagt tccagcatcc
    12361 cgctttcttt actgcgttcg ctaacggtaa aggtttcctg caacgcgtta atcgcttcca
    12421 gttcggtacg ctggctcagg acaaactgcg ttcctggttt ggccttaatg tccgcgatag
    12481 tcagcgcaac gccgtctttt tccagacgct ggccgaccat accattgacg gtgaaccctt
    12541 caccttccag cgtatagtgg ccgttttccc caactgtgag tgtcagttgc tgatcctgac
    12601 cattcagttg tgggatgtgc atccagctga tcgccagctc acctggtttc tctttggtta
    12661 atctcgccca gccgcgaccc acaatcggaa aatacttctg ctcaaccatg tcgcgcagat
    12721 tcagttcagc aatggtttta ccgagaatca tgcgcgattg cagcagttgg atctccggtg
    12781 ctgactcggg cgatgagtta gggatcatat cgctcaggcc gctgagaatg gcgttgccct
    12841 gtttctgctc aacctggacc agagtatctg cctgataaat tggcgtgctt aacagcgagt
    12901 aagcgacagc gatcagcgtg aacaacgcgg tcacgctgac aataaactta cggtgatccc
    12961 ataactcgcc gaccagacga agcagatcga tctcattttc ctgagtgctg cctggtggcg
    13021 tattcatatt tttagttgtc atcgtatttt ataaactcaa attaccggct gaggcgcttc
    13081 gcccattcct gactggcgcg ctccaacata ccgtagacat gctcaaatgc gtcctgactt
    13141 ttacgatagg gatccgggat ctctttctgt tccagccatt gcccaaacag cattgtcttc
    13201 ccgcggacct cgggagcgat tgccgtaacc tgagcaatat gttccgactc cattgccaga
    13261 atcagatcgt aatttcgtgc catctcagca gtgagcttac gtccggcatg tccttcaaga
    13321 gatacgccat gattagcggc aacatctgcc gctgtcgcat ctgcagggtg ctttaccaga
    13381 ccatgaacac ctgccgattt gacttttacg cctggcagac gtttacgcag caagcgctcg
    13441 ccaatgggcg aacggcaaat gttgccggta caaaccacca ggattgagtt aaattttagt
    13501 tgggccatgt ttgaatccac cgtgaagtct ctgtcaggtc atgcaccccg gaaatcgtcg
    13561 gtaccagtag agaaatcaca cggttccaac gagccagcgg cgcagtagtg acataaacaa
    13621 tgtcgtaagg ttcaagctgg aattccgttc cgagtatcat tgccgatgcg tccttcgcat
    13681 tcagctgata aatattggcg attttgccat tctgcttgtt ttgcgttgca cgaataacaa
    13741 aaatccccgt cgcatcagcg acatcctgat tcataccctc agcgttaccc agcgcttccg
    13801 ccagggtcat accgctgcgg tccattttca gcgtgctctg tttacccact tcacccatca
    13861 cgaagacttt cagatcatcg ttgcgcggaa taaataaaat atcgcccggg tgcagcagtt
    13921 tgttctggcg caagtcgcca cgctgcatca gcgcatagag gttcactttg gtctttacgc
    13981 cattctgggt gagcaccacg ttgcgccagt ctgcatctgc ggtcagcccc cccgcggcgt
    14041 taatcgcatc catgatggtc agaggaatat tggtaatcgg ctgctgaccc gatttgctga
    14101 cttcaccggt gacgtacgct ttttgcgagc ggaacgccgc gacgctgaca tcgacctgcg
    14161 ggctttcaat gactgaatcc agacgcgctg taatctcgtt gcgtacctgg gtaagcgttt
    14221 tcccggccac tttcaggcga ccaatatagg ggtagaaaat tgcaccatca gcgttaaccc
    14281 agttgccggt atcactggcg ctacggtatt gccctgcggg tgtggttagt tccgggtgat
    14341 cccagaccgt aaccatcaac acatcaccaa tgccgatacg atattcatag ttcgcgattt
    14401 gctgctccag ctccgggtta gcctgcgaca taatcggttt ggcgcgcagc tgatcaatca
    14461 atcctggcgt gacgggatag atattgacca ttttgtccag gtcatactga ttgtcaggta
    14521 gttcaatgac atccttgtta ctggtcgata aattctgacc aggaacaagg gtacatgctg
    14581 tcaaagacag aacagccaat gtcagtaccg aaaacttaaa aatgttcttt ttcattggtt
    14641 tatatttatc agtcattgta atgttatgaa ttacgtcagg ttattgtcca accggtactt
    14701 ctcgctctgc cgtcattgga tagagctggt atttgcgtga cagcatttgt ccaccatcac
    14761 gcgtcaatgg tgtccacgaa accaccgcgc ggttgcggtt aggtccaatg gtcatcaagt
    14821 cgaacggaat cgagatataa aaacctttac taaagccgcc ttcgccgtag tcatcttttg
    14881 atacgttact gattgccgcc cataccccta ctgccacgcc gctgtcaaag cgtttggcga
    14941 cgtcgatcgt tgcaccttta tcttttgcca gatattgccc aacgctaagt ttcatcagta
    15001 cgccattgag cgtcggcggg ttccagtaag ccgtcacgaa acctgttggc gtagaataat
    15061 cggtgaagcg catcatgttg tcccagtcac gctgcttcac gtagttaacg tccacaccca
    15121 gcgcccagct ggcatctagc gggcgataaa gcagctcgga accgacaccg gcatacatcg
    15181 tttccagata accgccatac acctggccat agaaaccgtt gcctaagtcg gcaaagtagt
    15241 tcgcttgcaa gttgttgaga taaacgtcat tgcgaacgta atcacggata tgcgtgcgca
    15301 cgcggggcag ggtagagtcc gcgggtaata gcgaagactt aaacttgtcg tagttattgt
    15361 aaatattggt gaaaataccg ccatcaagca gcaggtggtc ggtaaaccag tagcgggcgc
    15421 tggacatcag ccccagctgg aacatataga aatcttccgg gccgcccagc gactgtgaaa
    15481 gtgttgggtt gaaagagtag ctaaagcgat cttcacgaat acggtagccc cgaccaaagg
    15541 cagaaagatc ttcagcttca acacgttgtt gttgcagtgg ctctgattga ccaggcgctg
    15601 taccggccag ttgtttgcgc aggctggcta cgtcggtttc ggtagtcacc atcgccatat
    15661 gctcgcgctt ttgcgtcacg ctaatcttct caacgccttg cggcaggtta ttcaccagaa
    15721 tccggttggc acgatcgacc gcttcgcgag agtcacggta tttgtattgc tgaccagaca
    15781 tatacagcgt cttatcgcgt agctgaattt ctggcgcgtc aaagcccgcg ttatacttca
    15841 gggcggtaag ttggtttgct accgtggtgt actgcaatcc ttcagattcc ggcgcaggtt
    15901 gatatgccgg ttttggcgta tcgcgcaggg cagggcgcag atcgttgaaa ttggtccgta
    15961 acgtgaagcc aaacatcaac gtgttaccgc gttcataact caggttgaga tctgcccagc
    16021 tggcagcgcg atagactgcg ccgacgttga aatggcttgc ctgaggcagt ttgcccgcaa
    16081 aatcattctg gtaattgttg ccatcgtatt cgagtttcag acgcagagga ttccacggcg
    16141 tttgatactc aataccgcca aaaatagagg ctggaccacg aaagatatcg ctaaagctga
    16201 tatctcccgc atcgtgagac tctgcgcgat ggcaatattt atcgcttacc cgacaaaacg
    16261 ggttggtaat attgcccgca ttaccggcgt agccccaggc catcccgagg gtgaaatcga
    16321 acgggcccgc ttgcttgctg gccaccaaat attcaccgtc aaacagaccc gtaccagcaa
    16381 tatcacgttt accaaacgcc acttgcggta gccagtaacc ttcttcccac aggcgaagtt
    16441 taaaatcgaa agatttgtct ttgtatgact gatcgccgct gaaatcttcc cactggctat
    16501 atttgcgtgt gcgcacatcc gtataacgaa tggtgccttc cagccatggg aacagcgcca
    16561 cggaggtgga gtagaaacgg tattgatcgt tatcccggta gtttacgctg aattcacctt
    16621 ccggtgcgat gcgcgcattt ggcatctgca acaatcctgt gccgccaaag tctgattgcg
    16681 acgaacccag cggatccgga taggttaata cttcagcatg acaggcactg gagacggcaa
    16741 tggccaggca gcttaaaaga taagagttct tcttcatcag tcaggaacac gttgcgttaa
    16801 aacagacaca atctggtcat ttaaatcagc atatttttcg ggcagtacat gcgcagaaaa
    16861 gcctaaccaa agctggctgc caggcggtgg ttccacatgt cttttattcc acagggcaac
    16921 aggggcgacg acagcctcac cctcaggcgt aatcaccata acgttattct tgtccgcgcc
    16981 tgcaagacgg gtgtgatctt gcagataatc ggtaacgcta cggcctgcca gccagggcaa
    17041 ttgcccagcc ccagaaaccg cacctagcag agtaatagtc accggacgct gtacggtata
    17101 gagcgtatag tccccgacca gcgggggatt gctgttctca tccactcgta caaaatcagg
    17161 gtccagtttg accggtaagc gaccagtgat attgagattg agtaactgct ggcgcacgga
    17221 tttaatcgtt gccaccacat cgtcgtcagc ttctgcttcc caggaagcaa gctgtgccat
    17281 gacatgttgg tagtctttaa gcgctttagc tttcgccgca gagtcggtca gcaaggcacc
    17341 aggccaccag aggcgatcgc gcagttgcgg ttgtgtcacc aactggacaa cattttccac
    17401 ggggccaacg gaaagtgtct gttgttcgcc aggcagataa atagtcaccg ttccttgcgc
    17461 aaaggcatgg ggtgtcatta cgtaaagtac gctggcaatg aaatacgact gtaatttatt
    17521 catggtgcgg gtttaagaaa cgtcatttct acgggaataa cccctgcgcc taacatttgc
    17581 cgactttgac gaacctggcc agtagccgaa tcgacccaga atgtgttttg ccactgtttt
    17641 tctggcagcg tggaagtcac ctcttcctgc cagacattac acagcacatc gcgacccgca
    17701 atattcagcg tctctaatcc agcaaaggaa aaagtggaac tcagggtcgc agagcgaaaa
    17761 tggttgtctt cactccagag aatatcgcgc gtccagcggc tgccgtcttt tattgccagc
    17821 gcgttacgca gagggtcctg cccggaattt gtcacttcca gcagattatt attaagtttg
    17881 acggttttta aaagacgtcc attgtgggta accagcatgg cgttatcctg ggataaccat
    17941 ttgctttgtt cttgttctat atatcccagt accacaaaga ttcgttgccc ttcattaagg
    18001 cgtaaataca tcgtggaata aggcaacgcc tggatctgct gatccgctac ggtgatatcc
    18061 tgattatcga aaaggctggc gcgaaatgta tcgaccatac tttgctggct gtgcgtacat
    18121 cccgcaagaa atagtgcgaa aatcgataaa ataagagggc gcacctttaa tccttgaaag
    18181 gttaattaaa aaaatgcaaa tctctaaagc ccggtacgaa tatcgcaacc gggtatcatt
    18241 taactttaat tttgacgcgt taaagcagta ttagcgcgta gtggacgtgg tggttgaggt
    18301 gttatgagaa ccgccatcgc cactgtcatt tgctgcaact actgccaacg tcgccacacc
    18361 aacagccgtt gccacaccga caaccgtcgc cgtgccgaca gcgccagcag ccacgccacc
    18421 agtggtagca gcagtcgtgg ttgcacctgt agtggtcgtt gtggtgccag tagtacccgt
    18481 cgttgcctca gtcccgtttg ctgcttcagg ggcagcaaat gcagctggcg tcgtcaacat
    18541 gaaggccata aggattgcag aaagcttgtg tttcataact tttcctttat tcatcgcatg
    18601 gacaatacgt acgcttgaga tcaggattat ctactttgag tataacatcc ttgtgaaatc
    18661 tccctttcct cataccgcca gcactatata ctgcgggtgg gaaaggcgcg cagagggaaa
    18721 tataagattg ttaactattc ccctctggaa atacgacttg tgagcataag ataaatctta
    18781 gcaaaataca aattaaagat aagaaaattg ttaagaaagc tattaactat tgttggtaat
    18841 atagtttcaa gtggtactat aactggctgt taagtggcat ttataactat taggtgcaga
    18901 gatattcgct taaatggatt tgttgctcgg tgaaaaagta acttgcgtta taatttatgg
    18961 tgttgactat tgagctattc caataaaacc agccaacttt aaacaagcaa ctcatattaa
    19021 gagagataaa catttgccgc tgttggtcct cgcaggccat ttacgcggca aaattccacg
    19081 cgtaatcctg gtataagcac ttctgcgtcg cggggagtga atgcggaaat atggacctgg
    19141 acttctttac gaccgtcgga ggggataatg aatcctttgc cgcttttgcg atcaaaggtt
    19201 ttgacaattc ctgtcatttt acgggacaaa caaattcctt actgaaaata ctgcgctgca
    19261 ctatacgggg ttaataaaat aaagccagcg atatttaaga ccgccggacg gctaaaataa
    19321 aatttgctta atctcaatta tcatgcgtta atagctgcgt cggtttgaaa gacagacagc
    19381 atacaaagta gtttactaaa gcagttctca ttatcaggca ttatcccctt cttttgagtc
    19441 tctctcctga acactaagta gtttctgtac taaagccctg tttgccgaaa ggcccaaaat
    19501 gaaggaagta aaatatgtct aataaaatga ctggtttagt aaaatggttt aacgcagata
    19561 aaggttttgg ctttatcact cctgatgatg gcagcaaaga cgttttcgtc catttcaccg
    19621 ccatccagag caatgaattc cgcacgctga acgaaaatca gaaagttgaa ttttctattg
    19681 agcaggggca acgtggtccc gcggcagcga acgttgttac gctctaaggt tgccgttatt
    19741 actccacatc tccatttccg ctgtccatgt tgtcatggtt cacagtaccg cacatcggca
    19801 ttcgatgtga cggagcgaaa tcctttaggg gcaaagtgta ttttttgtaa atcgacgatg
    19861 atcacctttg ataacgtcgc gctgcaaata cgcactgacc atgcgccgct ggatttcaca
    19921 aaataatatc aggctccctc gtggagcctt ttttatatct gccttatttt tcttcaacgc
    19981 tgtatgtata gtaagcgata acctgttgat tattgaatct ttcggggaga tggcttataa
    20041 catttcttac ctgaccaggg taccgggaac caacacctta ctggcgtgtt gctgtctttc
    20101 aagaccagaa gaggttaaca gtgaatattg aagagttaaa aaaacaagcc gaaacggaaa
    20161 tcgctgactt tatcgcgcaa aaaatcgccg agctgaacaa gaatacaggg aaagaagtct
    20221 ctgaaattcg cttcaccgca cgagaaaaaa tgaccgggct tgaaagttat gatgtcaaaa
    20281 tcaaaataat gtgattttgt gaacatcacc cgtgcgaggt gatgttccgc ttgttgctaa
    20341 tttagtggcc aatcattggc gcttgtggaa ttaagcgtcg gtacaattcc tccggcaccg
    20401 ggctttgcca tactcccgca tacattgcgt aaccaatcac cgcaaacata atccccagaa
    20461 tcagtagcgt cattaaccag ccagacaacg caaaggcttt tttatttgcc gcaggttttt
    20521 gcagtgaaaa ggtcaatgtt gaggctaccg gacatgactc tacgcaagtc atacagccgg
    20581 tacattccac tgttcgtacc tgaattaatt tatcgaccgg gatccgtgat gggcaatttt
    20641 ttgcgcattt gccacagtcg atacaacttt cggcattgcg acgaatctta aacggcgata
    20701 atagcgaaac cacgcccatc agcgcgccat atgggcaaag ataacgacac caggcatggc
    20761 gaataaacag gctggcaatc agcaaaacga tcatgctgat taatgtcgcg gtccccatat
    20821 gacgaaagaa attgagcatt ttaacgtcca tcaccacgct gtagggcgac aacataaaat
    20881 agtgaatcgc ctgagcgggc atcaataacg caatatagag aaaaaaactc aacagcaaat
    20941 acttcacgcc gcgcagagga gtatccagcc agcggggaag gacaaattgc cgaccaaaca
    21001 gtttgttacc gagatcgccg attaattcag aaagcgtacc aaccgggcat aaccatgaac
    21061 aaaaggcctt ttttaataat agactgatga cgataaaagc gaccaataac agcatcgcgg
    21121 cggcgtggac ggacggtaac tggcctgttg caaggctata tttcagattc atcagaccgg
    21181 caatcggtag ccagccttcg atacctcccg gtctggtgac aaatgtcgta ctacctgccg
    21241 tttcgtaata gcgcacccaa taccagaacg tgatggcaat ataaatattc attgccaaca
    21301 gtaataattg cgtcgcttta cgccaggtcg tggcattgcg ccagtcattc cacggtaatt
    21361 tgccgcccgt cgtgcctggc cgccgctgcc agcgggttct tcttttctct gtcatgattt
    21421 tgccagtccg ttaagttgca taccaaatgc cactattcta gttgttctta actggctgat
    21481 attgattcaa atcgcgttca ggtctttctt atgcaaccat gcttccagag cggcaacact
    21541 gcgtgtaatt tcttcgtgtg gaaggggggc agataatggc tgctgctcca gttgtgcgca
    21601 tagctggctg gcgatacgca ttcccaggct cgagcaactg ctttttagct gatgtgcggc
    21661 acgctttatt ttctcgctat cctgattggc gcgggcaatg tcgatgtcat cgagaagcgg
    21721 cagggcatgt tgtgtaaata ataccagcca ttcgtggatc ttctccgtcc ccattaactg
    21781 agcatcttca ttgagttgcg atacatccag cggttgatcg ttattgactt gcagttggag
    21841 atagtgcgcc agtaactgac cgagcacttc acgcggcacc ggtttaggga taatcccgcg
    21901 aaatagcgaa ctggtacgct ggcgcaacgt ttcgtcgatg acatgggcgc taaagccaat
    21961 caaaaccagc gacggatatt gctgtgccag ttgccgggca agcgtaatgc cgtcgatatc
    22021 cggcagatca aaatccacca gtgcgacagc aaatggttca ctattttgca gtgtctctaa
    22081 agcctgcgcg gcatttccta cggcaacgac ctgcgcccca ctgctgttca acatctcaac
    22141 ggtaatccgc tgggttagcg ggttatcttc aattaacagc aaacgtaaac cgtccagacg
    22201 caccgcctga ttgactgttt ttggcacggg tgcagtggca atacgtaaca gcaagcgtaa
    22261 acaaaaacag cttccaacct ccggcgtgct ggtggcgctc agttcgccac ccatcgcctg
    22321 ggccagacgg ctactgatag tcagccccag cccggtgccg ccgcgtttgc cgctaacctg
    22381 aacaaatggc tggaagattt cagccagttt cgcgggatca ataccgcagc cgctgtcttc
    22441 cacttcgacc agccattgtt cgccatcagt gcgactacgc aggataatgt gcccttcgtc
    22501 ggtgaaacgc agggcgttgc tcaacaggtt ggtaatgacc tgacgaatac gctgcggatc
    22561 gcccattaac gcagtcggta catcgtcggc aattgccgtt gccaggcgaa tcgggcgacc
    22621 tttcacccgt ccgctcatta attgcagggt actttccagc aacgggcgcg gttcaaaggg
    22681 ttcatcgctg accgaaacat tcttgccgcc tgcttcgata gccgaataat cgagaatatc
    22741 gttgaggatg gtcagcaacg attcgccaga gtcggtaatt gcccgcaaat catcacgctg
    22801 agcgttaagt gcggggttat ctgccagtag ctgtgcggta ccgagaatac cgtacagcgg
    22861 tgtgcggatc tcatggctca tcgccgccag aaacgctgat tttgcctggt tggctttttc
    22921 tgcttccgcc cgtgcctgtc ggtgttctat caccagttcc tgcaattcag ctgtacgcgc
    22981 tttgacctgc gccgccaact gttcacggtg gcgattcagt gcatgaacac tgctgcgaaa
    23041 cgcatccatc agccgcccga tggtgtccag ttcccgcacg ccagcggttt ccgggaaagg
    23101 ggagtcgata tcaccatcca gcaagcgttg tagcgcctgc gtttgttctg caagagggcg
    23161 cgtgactgag cgataaacca cgcgccagag gatcagaatc agtgcgcaaa gagaaactat
    23221 tcccagcaat aacaggctgt attgcccgcg tgcactggct ttttccagat gtgccagccc
    23281 gtgctgatta cgcagctcaa tggtgtcgac cagctgactg acttcgctac taaactgcgc
    23341 gaattgggcg atgttatttt gggcgagagt ttgcaggtga ttgctgattt cactgtcctg
    23401 ctgatacagc gccagcaaat cgctatattg gctaacggta gttaacgttg ttgctacctg
    23461 agtacgaaca cccggatctt caatgcgtat ttgccgacgt tgcagaattt tcaccgcatt
    23521 attgagctgc ttttccagcg ttggcgcatt tttctggatc tgttccagcc ccagattcat
    23581 caccatttgc tgcacccgca gggcgctaag gcgcagttca ttcatctggt taacatactc
    23641 aagatcgata tcaatcagcc gatcgagtgc actttctgcg gcctgacgct gatgttgttc
    23701 gatcaaatcg taaatcccgg cctgggtcgc tccggcggag gttgccgcat tattcgcctg
    23761 accttgcgcc aggcgtgcga tttcatcggc ggcggcgact atctgctgac tgagttgctg
    23821 ttgttgctgg cgaagttgca aacgctgccc caccagttcc ccttgctgac gtaacgaacg
    23881 ggagatctcc tgctcctgtt gttcaatagc ggtggtgtca aaatcttgtt cccgtaacgc
    23941 ttgcagcaac gcattaatct tcaggctttg tgcggtgagc attcgcccct gcgcctgcca
    24001 catcttttcg ttatcggcac tggtcaggtt ctgcgcggcg aaaagttccc aggcgctggc
    24061 ttcgctcaac tggcgcgcca tattcatggt aggaatcaat gcctgagtgt tgtctttttc
    24121 cacctggctg ataaagcgca ggttgtacca tcccaccagg gtactggtca gggttaacag
    24181 cgccatcagg gcaaagccca tccagagtct tcgggtcagg gttaaattca cggtcggtgc
    24241 actttgggtg aaaaaggttg agtcgcaaag cggaatgcat ctagcataaa gccttattat
    24301 tgatgaggct atcatgcgcg tactgctatt gttactactt tcccttttca tgttatcggc
    24361 attttcggct gataacctgt tgcgctggca tgatgcacag cattacacgg tgcaagcctc
    24421 tacgccgcta aaagccaaac gcgcatggaa actgtgcgcg ctttatccca gtttgaaaga
    24481 ttcatactgg ttatcgttga actatggtat gcaggaggct gctcgccgct acggtgtgga
    24541 tttaaaagtg ctggaggcag gcggctacag ccagttggct acccagcaag cacaaatcga
    24601 ccagtgtaaa cagtggggcg cagaggccat tttgctcggt agtagcacga cgtcatttcc
    24661 cgatctgcaa aagcaggtag caaatctgcc ggtgatcgaa ctggtaaatg ctattgatgc
    24721 tccccaggtg aaaagccgcg ttggtgtgcc ctggtttcag atgggctatc aacctgggcg
    24781 atatctggtg caatgggcgc acggtaaccc actgaacgtg ctgttgatgc ccggacccga
    24841 taacgccggg ggcagtaaag agatggccga gggttttcgc gcagctattg ctggaagccc
    24901 ggtacgtatt gttgatattg cgcttggcga taacgatatt gaaatccagc gtaacctgtt
    24961 gcaggagatg ctggagcgcc atccagaaat cgacgtcgtt gccggaacgg ccattgcggc
    25021 agaggcggca atgggtgaag ggcgtaacct gaaaacgccg cttaccgtgg tgtcgtttta
    25081 tctttcacat caggtgtatc gcgggctgaa gcggggaaga gtgattatgg ctgccagcga
    25141 tcagatggtc tggcaggggg aactggcggt tgagcaggcc atcaggcaat tacaggagca
    25201 atcggtgtct gataatgtca gcccaccgat tttagttctg acgccgaaaa atgccgaccg
    25261 cgaacatatc cgccgctcgc tgtcaccggg gggatttcgt ccggtctatt tttatcagca
    25321 cacatcagcg gctaagaaat aaccttcgcc atgttgcgtc accagtaaat ccgcgctgag
    25381 tttatggcgt aaacgacgaa ttaacacatc gacggtgcgc aagtcagggt tttccacccg
    25441 acgcgcagag agcatacgca gcagacgttc acggctaaga atttcgcccg gattcgtcac
    25501 aaatgccacc aacatttcat actctgcgcg ggtcagttta atcggctcgc catcccgctc
    25561 cagcgtatgg cgcgacacat tcaggcaata accggcaaag cgatagcagt tgtcctgtgt
    25621 gtgcggttga gcttgtcgcg cgaggtcgat tcgccagagc agatttttca cccgtactac
    25681 cagttcgcgc agttccagcg gtttggtgac gtaatcgtct gcgcccattt ccagcccaac
    25741 aatacggtca atccgatcgc tgcgtcccgt aaccagaata atccccaccg ttgagcgttc
    25801 tcgcagggcg cgggttaaca tcaggccatt ttcatcgggc aaattaatat ccagcagaat
    25861 taaatccacc ggctgattct gcataatttc ccgcagcccg gcaccgctcg ccgtaacgga
    25921 aacggtatac ccctcctgag tgaagtagga ttgtaatcgc gcctgggtaa ccggctcatc
    25981 ttcaacaata acaatgtgat gtggcatcag agggttttac tcattctgtt catatctgtt
    26041 catattctgc cgtaagccgt tcatcctgac cagtgccgct gttcatattt actcattaag
    26101 atcgcttcac taaaccataa ttttacaggg gctattatgc ggaaactctg gaacgcgcta
    26161 cgccgaccca gtgctcgttg gtcggtacta gcgctggtcg ctattgggat tgtgattggc
    26221 attgcgctga ttgtattgcc acacgttggg atcaaagtca ccagcacaac cgaattttgt
    26281 gtcagttgcc acagtatgca accggtgtat gaagaatata aacagtcggt gcacttccag
    26341 aacgcctccg gcgtgcgagc tgaatgccat gactgtcata tcccgccgga tattccaggc
    26401 atggtgaagc gcaaactgga agcgagcaac gacatttacc agacctttat tgcccactcc
    26461 attgatacac ctgaaaaatt cgaagccaaa cgcgcggaac ttgccgagcg tgaatgggcg
    26521 cgaatgaaag aaaacaactc ggcaacctgt cgctcctgcc ataactacga tgcgatggat
    26581 catgccaagc agcatcctga agcggcgcgt cagatgaagg tggcagcgaa agataatcaa
    26641 tcctgcatcg actgtcataa aggtattgcc caccagttac cggatatgag tagtggcttc
    26701 cgtaagcagt tcgatgagct gcgcgccagt gctaatgata gtggtgacac gctgtactcc
    26761 attgatatta agccgattta tgcggcgaaa ggcgataaag aagcgtctgg ttctctgctg
    26821 cctgcttcgg aagtgaaagt ccttaaacgt gacggcgact ggctgcaaat tgaaatcact
    26881 ggttggacgg aaagtgcagg acgtcagcgt gtactcaccc aattcccagg taaacgcatc
    26941 tttgttgcct cgattcgtgg tgatgtgcag cagcaggtga aaacgctgga gaaaaccacc
    27001 gttgccgaca ccaataccga gtggagcaag ttgcaggcta ctgcgtggat gaagaaaggc
    27061 gacatggtga acgatatcaa accgatctgg gcttatgcgg attcgctgta caacggcacc
    27121 tgtaaccagt gccacggcgc accggaaatc tctcactttg acgctaacgg ttggatcggc
    27181 acgctcaacg gcatgattgg ctttaccagc ctcgataaac gtgaagaacg caccttgttg
    27241 aaatatctgc aaatgaatgc gtctgacacc gcaggtaagg ctcacggcga taagaaggaa
    27301 gaaaaataat gaacaataac gatctctttc aggcatcacg tcggcgtttt ctggcacaac
    27361 tcggcggctt aaccgtcgcc gggatgctgg ggccgtcatt gttaacgccg cgccgtgcaa
    27421 ctgcggcgca agcggcgact gaggctgtca tctcgaaaga gggcattctt accgggtcgc
    27481 actggggggc tatccgcgcg acggtgaagg agggtcgctt cgtggcagca aaaccgttcg
    27541 aactggataa atatccgtcg aaaatgatcg ccggattgcc ggatcatgta cacaacgcgg
    27601 cgcgtattcg ttatccgatg gtacgcgtgg actggctacg taagcgccat ctgagcgaca
    27661 cctcccagcg cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt
    27721 tctatgaaga actggaacgc gtacagaaaa ctcacgggcc gagtgccttg ctgaccgcca
    27781 gtggttggca atcgacgggg atgttccata acgcttcggg gatgctggcg aaagcgattg
    27841 ccttgcatgg taatagcgtg ggtacaggcg gagattactc taccggggct gcgcaggtga
    27901 tcctgccgcg cgtagtcggt tcgatggaag tgtatgaaca gcaaacctcc tggccgctgg
    27961 tattgcagaa cagcaaaacc attgtgctgt ggggctccga tttgctgaaa aaccagcaag
    28021 cgaactggtg gtgcccggat cacgatgttt atgaatatta cgagcagcta aaagcgaaag
    28081 tcgccgccgg tgaaattgag gtcatcagca tcgatccggt tgtcacatcc acccatgagt
    28141 atctggggcg cgagcatgtg aagcacattg cggttaaccc gcaaactgac gtgccgctgc
    28201 aactggctct ggcgcatacg ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta
    28261 actactgtgt aggttttgag cagttcctgc cgtatctgct gggtgagaaa gacggtcagc
    28321 cgaaagatgc cgcatgggct gaaaaactga caggtattaa tgccgaaacc attcgtgggc
    28381 tggcgcggca gatggcggcg aacagaacgc agattattgc tggctggtgc gtgcagcgta
    28441 tgcagcacgg tgaacagtgg gcgtggatga ttgtcgttct ggcggcgatg ttggggcaaa
    28501 ttggcctgcc aggtggaggt tttggttttg gctggcacta taacggcgca ggcacgccgg
    28561 ggcgtaaagg cgttattctg agtggtttct ccggctctac gtcgattccg cctgttcacg
    28621 acaacagcga ctacaaaggc tacagcagca ccattccgat tgcccgtttt atcgatgcga
    28681 tcctcgaacc ggggaaagtg atcaactgga acggtaaatc ggtaaaacta ccgccgctga
    28741 aaatgtgtat ttttgccgga actaacccgt tccatcgcca tcagcagatc aaccgcatta
    28801 ttgaaggctg gcgcaagctg gaaacggtta tcgccataga taaccagtgg acctcaacct
    28861 gccgctttgc cgatatcgtg ctgcctgcga ccacgcagtt tgagcgtaac gatctcgacc
    28921 agtacggcaa ccactccaat cgtggcatta tcgccatgaa acaggtcgtg ccgccgcagt
    28981 tcgaggcgcg caacgacttc gatattttcc gcgagctgtg ccgtcgcttt aatcgcgaag
    29041 aagcctttac cgaagggctg gacgaaatgg gctggctgaa acgcatctgg caggaaggtg
    29101 tacaacaggg caaaggacgc ggcgttcatc tgccagcgtt tgatgacttc tggaataata
    29161 aagagtacgt cgagtttgac catccgcaga tgtttgttcg ccaccaggca ttccgcgaag
    29221 atccggatct cgaaccgctg ggcacgccga gtggcctgat tgagatctac tcgaaaacca
    29281 tcgctgatat gaactacgac gattgtcagg ggcatccgat gtggtttgag aaaatcgaac
    29341 gctcccacgg tgggcccggc tcgcagaagt atccgttgca tctgcaatct gtgcatccgg
    29401 atttccgact tcactcgcag ttatgtgagt cggaaacgct gcgtcagcaa tatacggtag
    29461 cgggcaaaga gccagtgttc attaacccgc aggatgccag cgcgcgcggt attcgtaacg
    29521 gtgatgtggt acgcgtgttt aacgctcgcg gtcaggtgtt ggcaggggca gtggtttctg
    29581 accgctatgc acccggcgtg gcgcgaattc atgaaggggc atggtacgat ccagataaag
    29641 gcggcgagcc tggtgcgctg tgcaaatacg gtaaccccaa cgtgttgacc atcgacatcg
    29701 gtacttcgca gttggcgcag gcgaccagtg cgcataccac gctggtggaa attgagaagt
    29761 acaacggaac agtggagcag gtgacggcgt ttaacggccc tgttgagatg gtggcgcagt
    29821 gcgaatatgt tcccgcgtcg caggtgaaat catgaccacg ctgacagcac aacagattgc
    29881 ctgtgtttac gcctggctgg cgcagttgtt ctcccgtgaa ctggacgatg aacaactgac
    29941 gcaaatcgcc agtgcgcaga tggctgaatg gttttcgttg ctgaaaagcg aaccgccgct
    30001 cactgcggcg gtgaatgagc tggaaaaccg tattgccact cagacagtac gtgacgatgc
    30061 tcgtctggaa ctggccgctg acttttgcgg cctgtttttg atgaccgaca aacaagctgc
    30121 gctgccgtat gcatcggcct acaaacagga cgagcaagag attaaacgct tgttagttga
    30181 ggcagggatg gaaaccagcg gcaatttcaa cgaaccggca gaccatctgg cgatctatct
    30241 cgagttgttg agtcatctgc atttttcgct gggagagggg accgttcctg cgcgaagaat
    30301 cgacaatctg cggcaaaaaa cactgacggc gctgcgacaa tggttaccag agtttgctgc
    30361 gcgttgccat cagtacgaca gtttcggttt ttacgcggca ctaagccagt tattgctggt
    30421 gttagtggaa tgcgaccacc aaaacagata acgtcgtttg ttggcctgat aagacgcgtt
    30481 tagcgtcgca tcaggcatta gtgcgcagtt gccggatgcg gcgagaacgc cttatccggc
    30541 ctacgggaac agtgcttttt gtgggcctga taagatgcgt taagcgtcgc atcaggcatt
    30601 atggcgcagt tgccggatgc ggcgagaacg ccttatccgg cctacgggaa cagtgctttt
    30661 tgtgggcctg ataagacgcg ttaagcgtcg catcaggcaa caaactcacg ggtgagccac
    30721 aaaccgggaa agccgctggc gcagcaggcg gttttcctgc ttcaggtgcg caatatcatc
    30781 cattaacgtc agcgccaccg cgatccccgg ccagtccaga gccagttcat gacgcaggcg
    30841 taccgcgcgt tgcaccacaa tggcggcatg gtcgtcaaat acccaggttg tctcctggat
    30901 ctcccgcggt tcaaccaccc ccaaaccgac aatttcattc aactcctctt cagagatgcc
    30961 ggtatgcagg caaaattcgg taatagtaaa agtcaccgta acattagcca ttatgctttc
    31021 ccccaatctt tacgtggatc aaaagacgac tgggcgtctg ccagttgctg ccacagcgcg
    31081 gcagtgtttt catccggttt cggcggcatc acgattttca gtaccgcata cagatcgccg
    31141 gtctgttttt tgctcaccag acctttgcct ttaacgcgca atcgttgccc ggcctggctg
    31201 ccaggcggga tagtcagcaa aatgctttct ttcagtgttg gaacggtgac tttagtaccc
    31261 agcgccgctt cccacgggct aaccggcacc actatttcca gatcctggcc gacaatatca
    31321 aacagcggat gtggcgcaat gtgaatcacc agccacaggt cgccatttgg accgccgttt
    31381 tcgcccggcg tcccctggcc tttcaggcgg atgcgttgac cattgccgac gcccgccggg
    31441 atcttcacat tcagcgtttt cgggatttcc tgttcgatca tgccaaaggc gttataaacc
    31501 ggcaggttat agctgatggt acgcttatgc tcagtaagcg tttcttcgag gaaaaccgcc
    31561 acttcgattt caatatcgtg gccgcgtgtg gcggggcgtt gacggctctg gcgggcatgc
    31621 tgaccgaaaa ttgacgagaa gatatcgtca aaatcttcgg cgttaaaact ctgaccgtcg
    31681 ccatgatgga actgacggtt aaattgcgga tcgttgcgat gttgccacat ctgatcatac
    31741 tcagcgcgac gttgttcatc acttaacact tcccaggctt cggcgacctc tttgaagcgg
    31801 gcttcggcat ccggttcttt gctgacatca ggatggtatt tgcgggccag tcgacgatag
    31861 gcggtcttga ttgtcttgag atcgtccgtc ggtttcacgc ccatgatggc gtaataatcc
    31921 tttaattcca tagcgttatc tcgcgtaaat caacacaaat tgaaggaacc cctgtaaggt
    31981 aactcctata agtgtagggt aatcctcaaa atttcatatg ccaacacaga atatgttatt
    32041 gaaatcatcg cggaggggag gtcgcctcaa ggtgggagga acatatttta tttacctccc
    32101 tcctgaactt ttggctcatg cttacaagtt aaacaataag gaaaggaatg cacaactcga
    32161 ctatttaaat cgttatgcaa tttaatgata aagacatgtg ctaaaaacca tttctattag
    32221 tctcacattc tttttttatt ggtaatggta acgccgctga ttttctttat agctactgat
    32281 gtaatcatta gcggcgtaaa cgaggccggg gctaatcagg catagtttgc gtcaaaactt
    32341 gcctgttttt aaaggagaat atagaaaaac aggcgttcaa cgagccattt tgcgaacctg
    32401 ttcccggaaa aatgacatat ttctgtcaca ctctttaatt attgataaca aaagaggtgt
    32461 gaggaatgaa caaaacgcta atcgccgcaa ctgtggcagg gatagtttta ctcgcttcaa
    32521 atgctcaggc acaaaccgta ccggaaggct atcagctaca gcaagtcctt atgatgagcc
    32581 gtcataactt acgtgcgccg ctggcgaaca atggcagtgt gctggagcag tcgacgccga
    32641 ataaatggcc tgaatgggac gtccccggtg ggcagctcac cactaaaggg ggcgtgctcg
    32701 aagtgtatat gggccattat atgcgtgaat ggctggcaga acaggggatg gtgaaatcag
    32761 gggaatgccc gccaccggac accgtttatg cctatgccaa tagtctgcaa cgcaccgtcg
    32821 cgaccgcgca gttctttatt actggcgcat tccctgggtg tgatattcct gttcatcacc
    32881 aggaaaaaat gggcactatg gaccccacct ttaacccagt gatcaccgat gattccgccg
    32941 cattcagtga acaggcggtg gcggcaatgg agaaagagct cagcaaactc cagcttaccg
    33001 acagctacaa gctactggaa caaatcgtta actataaaga ttctcctgcg tgtaaagaga
    33061 agcaacaatg ttcgcttgta gatggcaaaa atacctttag cgcgaagtat caacaagaac
    33121 cgggcgtttc cgggccgctg aaagtcggca actcgctggt agatgcgttt actttgcaat
    33181 actacgaagg ttttccgatg gatcaggtgg cctggggaga aatcaaatct gaccagcagt
    33241 ggaaggtgtt gtcgaagctg aaaaacggtt accaggacag cctgtttacc tcaccggaag
    33301 tggcgcgcaa tgttgcgaaa ccgctggtca gttatatcga caaagccctg gtcaccgatc
    33361 gcaccagtgc accgaaaatc acggtattag ttgggcacga ctccaacatt gcctctctgt
    33421 taacggcgct ggatttcaaa ccgtatcagt tgcatgacca gaacgaacgc acgccgattg
    33481 gcggcaaaat cgttttccag cgttggcatg acagcaaagc caatcgcgat ttgatgaaaa
    33541 ttgaatatgt gtaccagagt gcggaacagt tacgtaatgc cgatgcgtta accctgcagg
    33601 cacctgcgca gcgcgtgacg ctggaattaa gcggttgccc gatagatgcc aacggttttt
    33661 gcccaatgga taagtttgat cgcgtattga atgaagcggt gaaataacag aaaactcccc
    33721 cgcgtgaagc gggggagtcg ctggttaaac gtttttacgt tcgatggtct gttcgcccca
    33781 aaaaagcgaa tctttatcgg tcttagcaaa ggctttgact aacacttcat cgctaccttc
    33841 ttcccaaatc ttttccgcca ttttttcgtc gtacccggcg acttcgaaaa tggcctcggc
    33901 tatttccggc gacgtattgc gcagagatgc ccattcaccg acgtgatgag ctttcgcttc
    33961 ttgagttggc atgcgtatcc tcctgttgaa gattagctgt taagtttaac tgccagacct
    34021 gcgacatatt ccccttgata acgagcaata gacagttctt cctggctggg ctggcgtgaa
    34081 ccgtcaccgc ctgcgatggt ggttgcgccg tacggcgtac cgccgcgaac ctgtgaaacg
    34141 tcaaataatt cctgcgctgc gtagccaatg gggacaatca ccatgccgtg atgcgcaagg
    34201 gtcgtccagg tggatgtgat ggtttgttcc tgaccgccgc cagtaccggt ggaactaaag
    34261 acgctcgcca gttttccgta tagtgcgccg gaagcccaca ggccgcccgt ctggtcgagg
    34321 aaggtacgca tttgaccgga catgttacca aagcgggtag gtgtaccaaa aataatggcg
    34381 tcgtaatcgg ccagttcttg cggggttgca accggtgcag tttgcgtttt accgcctgct
    34441 ttttcaaata attgcggcgg catggtttcc ggtacacgct taacgacaac ttctgcgcca
    34501 tccactttgc ttgcaccctc agcaactgcg cgtgccatcg tttcaatatg tccgtacatg
    34561 gaataataaa gcaccagaac tttagccatt tctaaccact cctcgtgtta tctctattcc
    34621 gtagcgattc gctaccactt atttaaagat aagacgtcct tttcagagtg caaatttcac
    34681 aaccacttat ttgatttata acaactttca caagcacgta attttgtcgc aaaatgacac
    34741 atttttatct catcgcgttt ttttaatcat aagagcggct tatggataat tattggagat
    34801 gatatctatt ctcgctaaga agctgttgca ggatattact aaacgcgggt ctgcccgcgt
    34861 cagttcacta agcttagtcc cacgtagcga aaatatggca gccgccatac gccgcgttaa
    34921 ttctatgcaa tatgatgtct atacccagac ggaggtcagt aatggcaaac catcgaggcg
    34981 gttccggcaa ttttgcagaa gaccgcgaaa gagcatcaga agcaggtaaa aaaggtggac
    35041 agcacagcgg gggtaatttc aaaaatgatc cgcagcgcgc atctgaagca ggtaaaaaag
    35101 gaggcaagag cagtcacggt aaaagcgaca actagccggg ctaatccatg acgaatgtat
    35161 ttttgtctga aactcgtcaa aaagtcatca ccgccggtta ccccggcggt tttctattta
    35221 tggctcctga tgaacaactt ctggcggtgg aacgtcaacc aaccttctgc ttaacaacgc
    35281 attgagtaaa atcgcgccaa aggtggctgt accgatccct cccaacgtaa aaccgcccag
    35341 tgtgagagca aaatcacccg cacccagcac taaggttact gcgaccataa tcaaattacc
    35401 gttctggctt aaatccacac tgttttgtac ccagatccgt gcgcctgcga cggcaatcag
    35461 cccgaacaca acaattgatg caccaccaat caccgcggcc ggaatagtat ggatcagcgc
    35521 gccaaatttc ggcgaaaagc ccaacagcat ggcgatgacg gcagcggcaa caaacaccag
    35581 cgtcgagtag acttttgtca cggccatcac accaatattc tcggcgtagg tggttacgcc
    35641 gctaccgccg acagagccag aaagcatcgt cgccagccca tcgcctacga atgcccgccc
    35701 catatacggg tccatattgc gtccggtcat cccggcgacc gccttgagat gacctaagtt
    35761 ttccgccacc agaatcaccg ccacgggcgc aatcagcatc attgcctgac cattaaaagc
    35821 aggagtggaa aaatgtggca gaccgaacca ggcagcatgg ctgacgagag taaaatcgac
    35881 ggcttttccc agccctaaaa cgttggtcat cacgccatac agcagacagg cgacaattaa
    35941 tcctacgaga atcaataatc gctggatcat gccacgggta aatacggcca ccagcccaat
    36001 acacagcacc gtcattaccg ccatccagct atcaaaggcc gaagccgata catttttcac
    36061 tgcgataggc gctaagttca ggccaatcgc catcactacc gcacctgtca caaccggtgg
    36121 cattagtcgt tcaatccagc gcgtaccgat tttcatcacc accaggccaa tgacggtata
    36181 aaccagccca caggcgataa tcccgcccag cgcgatgctg atattcggat taattccctg
    36241 accattaaag cccgtcgcgg caatcaccac gccgacaaaa gccgcgctgg aaccgagata
    36301 actgggcacg tgcccgccgg taataaagaa aaacagtaac gtgccgatcc ccgacattaa
    36361 aatggaaaga ttgggatcca gccccatcag aattggcatt aacaccgtcg cgccaaacat
    36421 cgccaccgcg tgttgaacgc ccattactgc cgtttgagca aacggcaatc gttcatccgg
    36481 cgcgaccacg ccgctctctg tagaggtcga ttttaactgc cagtgaggaa aaccgaacat
    36541 tgccatcagc tgtctcctta aggaggttaa caagcagggc gcatcagcgc gtgataactg
    36601 cgatcgaacc acaccagccc gtagggtgtg gcgtgacgat gaatcgcttc gatggcgcaa
    36661 aacaaaatgt cgtgggtgcc gacgctcacc acctggctga tacggcagtc aaacgaaacc
    36721 agtgcctctt ccagttgcgg gcatccggtc acaccagtct gccagcgggc ggcggcaaag
    36781 cggtgttcca tgggcgtttt gccgccaaaa aggtttgaaa gcggctcctg cccggcgcta
    36841 agtgtattta cacacagcgt tcgattttca ttgaataccg gccagacgga cgccccacga
    36901 ttcaggcaca ccagtaatgt gggcggcgtg tcagtcacgc tgcagacggc gctggcggtg
    36961 aacccggcgc gcccggctgg accgtccgta gtgatgatat tgaccgccgc gcccatacag
    37021 gacatcgcat cgcgaaaggt ttgttgatcg acaatgttca ttgtttgctc cttacaacag
    37081 cccgcaggct tcttcaaagg acagacgtgg caggcgcgca aaaagtttgc tggaatcgcc
    37141 atagccgata ttaatcagca gattgctctt cagcgtgctg cccgcaaaaa aggcgtcgtc
    37201 aacgtattga cggtcaaagc ccgacatcgg gccggtatcc agccccagcg cacggcaggc
    37261 gacgatcaga taggccgcct gcatggaact gttgcgaaac gctgtttctt cggcaagttg
    37321 tggactggag gtaaaccaac tgcgggcatc gccgtgagga aacagctgtg gtaaccgttc
    37381 ataaaattca ctgtcccagg cgacgatagc ggtgacgggc gcggtcaggg ttttttgcag
    37441 attgccgctg gaaagcgccg ggcgcagacg ttcttttcct tctgcagtgc ggataaacac
    37501 gatccgtgcc ggagaacagt tagctgatgt cggcccccat ttcatcaggg cataaatctc
    37561 ccgtaacgtc tcatcgctga cgggtgtctc ccgccagccg ttgtgagtgc gggcatcggt
    37621 gaacagggtg ctaagcgcac ctgggctaac ggcttcgttc atagcaattc cttacagagc
    37681 ggcttcacgg tgatgtaaca ggctggcaag cccgttgagt aacagagcat taaacgtttc
    37741 gggatcggtc acgttgcagg cgtgtccgcc atagcgcatc accattttct ggctatcggg
    37801 cagggcggca tgaagttcac tggaacatgc tgttggcacc agcagatcat cactggcgca
    37861 gatgatttgc accgggcagc ggatacgatc cgcatggtga ctaaagtcag cgcgtttgag
    37921 ggcgttaagt cgacgcagta aattattttt gccttgaaaa tgcgccagtg ccagcgcgtc
    37981 ttctgcctca aggcgaggtg cgcgggccgc catccagtcg gcgggataga ggaacaacgg
    38041 ctgcgcttcc acccatgcct gcgcgccgcc gctatgtagt aactgttcgc gaacctgaaa
    38101 acagcggcgc gtatgggcgt ttattcgtag ccagccgtta acgctgacca gcacagttac
    38161 cgacgcggga taatccagcg ccagctgcat tcccaccagc gcaccgagcg catggccgac
    38221 caccgcgtaa cgctcaatcc ctgcggctac cagcgcctga tgcagttccg ctgccatctg
    38281 ggcgatactg taatcttctg ccagcgtgtc gggattattg ccggtgccgc gctggtcgta
    38341 acagactacc tgatactcct gaaccaacac cgccagttgc ggtaaccagt aactgccgct
    38401 accgccaaga cccgaaatca acaccactac gggcgcatca gcataagggg gaggtgagag
    38461 tgaaagtttc atcgcggcct cacttggcga tatgcgcaat tgtggcgatt tccaccagcg
    38521 cgtcaggttt taccagtccg cactgaatgc agaatcgcgc cggtttgtca cccgggaaaa
    38581 attcggcgta gatttcgtta atcgcggcgt aatttttcca gtcggtaata aagatgctgt
    38641 tgaaggtcac atccgccatt gtgccacccg ccgtctcgat gaccttgcgg atggtttcca
    38701 gaacgtggcg ggtttgcgcc tttgggtcat cggcaaacag cacgttgtta tgttgatcaa
    38761 aagccagcgt accggagaca tacaccacgc catcagccag cgtgccggga acgaaggggg
    38821 ccagcggtgc gctgctgcca gcaggaataa ttacggattt tggcatcgtt aaactcctta
    38881 agcgatacga gcaaaggacg tggaagaaag cgcgtcgcag aacgtttcga cgtcgctgac
    38941 ccagccaaaa aaggtttcga tattgaacaa cgcggctttc tgtgcaaatt ccggtcccgc
    39001 ctggtgtgtt gcgtcttcca gcaccacgcc gaaatactcc agaaaaaagc cgtcgcgtag
    39061 cgtcgattcg acgcagacgt tggtagcgat accggtgaaa accagatggc gtattccacg
    39121 gctgcgcaaa atgctgtcca gcggcgtatt gaagaaaccg ctgtagcgcg gcttcggcag
    39181 cacaatatcg ccaggctgcg gcatcagttc atccaccagt tgataatccc aggagccttt
    39241 cgccagcaat ttcccctgca gctgcggctg cttacgcatg gttttcaggg cgttcgattt
    39301 atgaaaattc ggtgagccgg ggccgccagc ctcgacatac tgttcatccc agccattttg
    39361 aaaccagatg atcaacatcc ctgccgctcg cgctgcggtc acggcggttt gaatgttggc
    39421 aatgaccggg cgagtggttg agacatcaaa cccggcgaga tctaagtagc cgcctggcgt
    39481 ggcataagcg ttttgcatat ccaccacgat cagcgcagtt tgctgcggat cgaaggtaat
    39541 ggcttccggt cgtgcggtta aggtcgtcat catgccacct cctgagtcag cgcagggaga
    39601 tgggcgcggc actgcatcag tggttgaatg cgctcgccga agttttcgat tcccgacaga
    39661 aaatcatcga aggtcaacag cacgccttcg gcaccaggca cgcttgcgac ttcatctaac
    39721 atgcgcgcga cactggcgta agaaccgact aacgtcccca tattgatgtt taccgccgaa
    39781 gtgggatcgg ccatctgacg gacgttggtg tcagtatcgg agcgggtatc tttctgactt
    39841 tgttcggtta gccagcttaa cgcctcttca tccgcgcccg ctttgtagtg ttcccatttt
    39901 gcatgagcgg catcgtcggt ttcgtcggca atcaccataa acaacacata agagccaacg
    39961 tcgcgtccgg tttgctctgc ggcctgtttc atccgcgcag cggtcggggc gaaagccgtg
    40021 ggtgtattta cgcctttgcc gaaacagaag ttaaaatcgg cataccgggc ggagaacgcc
    40081 atgccagcgt cgctttgccc ggcgcagatc actttcatgg ggacactcgg ttgcggactg
    40141 acgcgacaat catccatagt gaaaaaatcg cctttaaaat cgcttttccc cgagccccac
    40201 aggtcgcgca gcacctgaac atattcggtg agagagtcgt aacgacggga gaaatagtca
    40261 tcgccaggcc agatccccat ctgctcatac tcgggcttct gccagccagt cacgaggttg
    40321 acgccaaaac gcccgccaga gatggagtcg atggttgcgg ccatacgggc gacgattgcc
    40381 ggaggtaacg ttaaggtggc agctgtcgcg tatatctgaa tgcgtgaggt gacggctgcc
    40441 agccccgcca tcaaggtgaa cgactcaagg ttgtgatccc agaactcagt tttgccgcca
    40501 aagccacgca gtttgatcat cgacagggcg aaatcgaaat ggtagtgctc cgctttttgc
    40561 acaatggctt tattcagttc aaatgtcggc atgtactgcg gcgcgtgggt cgaaatgagc
    40621 cagccgttgt tgccaatagg tacgaatacg ccaattttca tcatcaacct ctcttcgtct
    40681 cgtaaagtga aagtcagacg gggcgctgca tcctgcatat ccttttcagc cgcgtaatgg
    40741 cttgtttgca aagcggatgc cagtttttaa aaagtgaatg ttattaatgt gttaatgtta
    40801 agttgtctaa aatgcctgaa aaaaagtgga ctaaacggtc aaaacagttg cacataaaac
    40861 atgcattcgt gcgcgatgag agtgcagaag gtcgaggccg ggcgggggtt ttgctatcct
    40921 gttgccaatc aacaagaggg gagagcgcat gacgcaaggc gcagtgaaaa caacgggtaa
    40981 gcgttcgcgc gcagtaagcg cgaagaaaaa agcgattctt agcgcagcac tggacacttt
    41041 ttcacaattc ggttttcacg gcacaaggct ggagcagatc gcggagttgg cgggtgtttc
    41101 aaaaaccaat ctgctgtatt actttccgtc aaaagaggcg ctgtatatag ccgtactgcg
    41161 gcagattctc gatatctggc tggcaccgtt aaaagcgttt cgagaagatt tcgccccgct
    41221 ggcggcgatc aaagaataca tccgtctgaa gctggaagtt tcccgcgatt atccgcaggc
    41281 ttcgcgcctg ttctgtatgg agatgctggc aggtgcaccg ctgttaatgg atgaactgac
    41341 gggcgatttg aaggcattaa ttgatgagaa atcggcgctc attgccggtt gggttaaaag
    41401 cggcaaactc gcgccgattg acccgcagca tttgattttt atgatttggg cttccactca
    41461 acattacgcc gattttgccc ctcaggtgga ggcggtgaca ggcgcgacgt tgcgcgatga
    41521 ggtgtttttc aatcaaacgg ttgaaaacgt gcagcggatt attattgagg ggattcgacc
    41581 acgttaaaga tgccggagga ggttgtaaca tcctccggct acctgtttaa ccgatcgtca
    41641 ttaaactggc gttaccgcca gcagcagcgg tattcacact cagcgaacgc tcgatataca
    41701 gccgttccag aaggatattg ctttcgccac gggcaaaacc ctgcaccgaa acaattgcac
    41761 catcccgcgc ggcaacggct tcacacaatg cgcgaagctg atccgaatca ccgtggaaga
    41821 tcaccgcatc aaatggttga gcggttatat tttccgcttt cgccagttga atacgttcgc
    41881 tgactgccga tggcaatgcc ttcactaact gacgatgcag cgcatcatcc ggccacagca
    41941 cctggctacc caccgccagc acggcggcga gctgagtcag cgcatcctgt tcatcatccg
    42001 caatacacaa cacgcgctca cgcggcagca gcgtccaggt gttgcgttca cccgtcggcc
    42061 ctggcaacaa tcgttgtgtt cctgcctgcg ccaactcgcc atattgcgta cataacgcct
    42121 gcaattctgg acggtttgcc gcccattccc gcagtgcatt tagcggctga gtcaatgcgg
    42181 ctttcaactg cgcatcgacc ggatactccg catcctgacg cgcgagcgtc actgccagcg
    42241 cactttccgg gcgattcgcc agcagacggt agagatagag cggaccgcct gctttcggcc
    42301 cggtaccgga caacccttcg ccgccgaacg gttgcacccc aaccactgcg cccaccatat
    42361 tacggttaac gtacaggtta ccgacatgcg ccgagccagt gacctgggcg atggtttcat
    42421 caatgcgcgt atggacgcca agcgtcagac cataaccgga agcgttaatc tgctcgatca
    42481 gcgccggcag ctggttacgg ttgtaacgca ccacatgcaa caccggacca aagacctctt
    42541 tttgtaattc ggcaaagtca tccagttcga tcagcgtcgg agcgacaaag gtgccgcttt
    42601 gccattcacg ggcatcttcg ctgttttccc gcaccgcctg gaacacctga cggcctttac
    42661 tacgcatggt ctgaatatga cgctcaatat tggctttcgc ttcgctatca atcaccggac
    42721 cgatatcggt ggtcaggcga cccggattac ccatccggca ttcggccatt gcgccgcgca
    42781 gcattttcag cgtgtggtcg gcaatctcat cttgcaggca cagcacgcgc agtgccgaac
    42841 aacgctgacc tgcactgtcg aacgccgagg ccagcacatc tattacgact tgttcggtca
    42901 gtgctgaaga atcgacaatc atcgcgttca tgccgccggt ttcagcgatg agcggaatag
    42961 ggcgaccctg agcgtccagg cggctggcga tattgcgctg cagtaacgta gcgacttcgg
    43021 ttgaaccggt aaacatcacc ccgcgcacgc gatcatcacc cgtcagttgc gcgcccacgg
    43081 tttcaccccg acctggcagc aattgcacca cgcctggcgg tacacccgct tccagcaaaa
    43141 tggcgatccc ttgcgcggca atcagcggcg tttgttctgc cggttttgcc agcacgctgt
    43201 tacctgccgc cagtgcggca gcgatctgcc cggtgaaaat agccagcggg aagttccacg
    43261 gactgataca taccacaggc cctaatggac ggtgggtttc gttagcgaaa tcatcccgca
    43321 cctgtccggc atagtagtgg agaaaatcga ccgcttcgcg cacttcggca atggcgttac
    43381 tgaaggtttt tccggcttca cgcaccagaa taccaatcag ttgctgcatc tggctttcca
    43441 ttagcacggc agcgcgatgc aaaatcgccg cgcgttcagc cggaggagtg gcaaaccaga
    43501 ttggcgcgtt attaaccgca ctttccagcg cctgttctac ttcacgcggc gtggcttcac
    43561 gcacaaagcc cacaatatct ttcggttccg cagggttaat aacgggcgac atctcaccag
    43621 ccgcgaccga ttgttccagc attggcaagg cctgccattt ttgcagtgca ctattgagca
    43681 gggcagaaga gagcgaggcc agccggtgtt cattggcgag atccagccct gccgagttgt
    43741 cgcgcccgtc accgtaaaga tcgcgcggca ggggaatttt cggatgcggt aatccagttt
    43801 gcccttcctg ttgcgccagt ttttctacag cagtgaccgg atcggcaacc agttcatcca
    43861 gtggcaaaga ggtgtcagca atacggttaa caaacgaggt gttagcaccg ttttccagca
    43921 ggcgacgcac cagatacgcc agcagcgttt catgggtacc gacaggagca taaatacgac
    43981 acggacggtt aagtttgccg tcggcaactt tcccggtgac ctgctcatac agtggctcgc
    44041 ccataccgtg caggcactgg aactcgtact gacccgggta gtagttctga cccgccagtt
    44101 gataaattgc cgccagcgta tgggcgttat gcgttgcgaa ctgcgggtag attagattcg
    44161 gcaccgccag cagctttttc gcacaggcga gatatgaaac gtcggtatac accttgcggg
    44221 tataaaccgg ataaccttca aggccgtcca tctgcgcgcg cttgatttca ctatcccagt
    44281 acgcgccttt caccaggcga atcatcagac ggcgacggct gcgggtggcg agatcaatca
    44341 ggtaatcaat caccggtggg cagcgttttt gataagcctg aataacaaaa ccgatgccgt
    44401 tccagcctgc cagttccggc tcgaaacaga gtttttccag cagatcgagg gagatctcca
    44461 gacgatcggc ctcttcggca tcgatgttaa taccaatatc gtactgacgc gccagcaggg
    44521 tgagtgattt cagacgtggg taaagctctt ccattacccg gtcatactgg gcgcggctgt
    44581 aacgcggatg cagcgccgac agcttgattg aaatgcccgg accttcatag atgccacgac
    44641 cattggacgc tttaccaatg gcgtgaatcg cctgctgata ggaaaccata tacgcctgtg
    44701 catctgcggc ggtcagcgcg gcttcgccca gcatatcgta ggagtaacgg aaacctttct
    44761 cttccagctt gcgtgcattg gctaacgctt ctgcaatggt ttcacccgtg acgaactgct
    44821 cgcccatcag gcgcatcgcc atatccacac ctttgcggat cagcggttca ccgctcttac
    44881 cgataatgcg gttcagcgag cgggagaggc tggcttcgtt atgggtggaa accagtttgc
    44941 cggtaaacag cagcccccag gtggcggcat taacaaacaa cgacgggcta cgaccaatgt
    45001 gtgactgcca gttaccgttg ctgattttgt cgcgaattaa cgcatcacgg gtggctttgt
    45061 cgggaatacg caacaacgct tctgccagac acatcagcgc cacgccttcc tgcgatgaca
    45121 acgaaaactc ctgcaataac ccctgaacca tacctgcgcg accactggcg tttttttgat
    45181 tacgcagttt atcggccagt tgatacgcca gtttgtgcgc ctgttcagca actggctgcg
    45241 gcaggcgagc ttgttccagc agcatcgaaa ccgcttcggt ttccggacgg cgataggcag
    45301 cggtgatcgc ggcgcgggaa accgactggg gcaaaatttg ctcggcaaag tcgaggaaag
    45361 gctggtgtgg ttcctctgcc ggagtcggtg cttcatcgct ctcattggca gcgccagaaa
    45421 gcagcgcagg tagctccggc agagtatcgc tgttttccag ttgttcaaga taagaaaaaa
    45481 tcgcctgttt aattaaccag tgtggcgtgc gatcgatacg tgtcgcggca gacttaatac
    45541 gctcacgcgt cgcgtcgtcc agcttaaccc ccatggtggt ggttcccatg ccattactcc
    45601 tgttgttctg aaaggtgcaa cttaacgtta tcgtgaaata tccatgatgt tgcaactttg
    45661 tgcaactgtg ttaaatgtga cctgcgtagc aagcttaaaa atgaatgaaa tgttaataaa
    45721 cgaaatcgat atgacaggga ttaaaaaaat aactcagact ttttctctgc ggcagttaac
    45781 atttttgaaa ggtgcaaccg caaaaaatgt gagagagtgc aacctgatga aaaatagtgt
    45841 cgctgagcac taaaatttaa tgtaaatggt gtgttaaatc gattgtgaat aaccagcgct
    45901 tccggcagga tacggtcgcc ctggtaaaac ataaactctg ttaccccgtt ccggtggcag
    45961 atataacggc aagtttcgac attgccgata ataatttttt ggagacttta gatggctatt
    46021 agcacaccga tgttggtgac attttgtgtc tatatctttg gcatgatatt gattgggttt
    46081 atcgcctggc gatcaacgaa aaacttcgac gactatattc tgggcggtcg tagtcttggg
    46141 ccattcgtga cggcattatc ggcgggagcg tcggatatga gcggctggct gttaatgggg
    46201 ttgccgggcg cggtttttct ttccgggatt tccgaaagct ggatcgccat tggcctgaca
    46261 ttaggcgcgt ggataaactg gaagctggtg gccgggcggt tgcgtgtgca taccgaatac
    46321 aacaataacg ccttaacatt gccggattat ttcaccgggc gctttgaaga taaaagccgc
    46381 attttgcgca ttatctccgc gctggttatt ttgctgttct tcaccattta ttgcgcttcg
    46441 ggcattgtgg caggcgcgcg tctgtttgaa agtacctttg gcatgagcta cgaaacggct
    46501 ctgtgggcgg gggctgcggc gacgatcctt tacaccttta ttggcggttt cctcgcggtg
    46561 agctggactg acactgtaca ggccagcctg atgatttttg ccctgatcct gacgccggtt
    46621 atcgtgatca ttagcgtcgg tgggtttggc gactcgctgg aagtaatcaa acaaaagagc
    46681 atcgaaaacg tggatatgct caaagggttg aattttgtcg ccattatctc gctgatgggg
    46741 tgggggctgg gttacttcgg gcagccgcac atcctggcgc gttttatggc ggcagattct
    46801 caccacagca ttgtccatgc gcgtcgtatc agtatgacct ggatgatcct ctgcctggca
    46861 ggggcggtgg ctgtcggctt ctttggtatc gcttacttta acgagcaccc ggcggtagca
    46921 ggtgcggtaa accagaacgc tgaacgcgtg tttattgaac tggcgcaaat tctgtttaac
    46981 ccgtggattg ccgggatttt gctgtcggcg attctggcgg cggtaatgtc aacgttaagt
    47041 tgccagctgc tggtatgttc cagtgcgatt accgaagatt tatacaaagc gtttctgcgt
    47101 aaacaggcca gccagaaaga gctggtgtgg gtagggcgag tgatggtgct ggtggtggcg
    47161 ctggtggcga ttgcgctggc ggcaaacccg gaaaaccgcg tgctgggctt agtgagctac
    47221 gcgtgggcag gctttggcgc agcgtttggt ccggtggtgc tgttctcggt gatgtggtca
    47281 cgcatgacgc gcaacggtgc gctggcgggg atgatcatcg gtgcgctgac ggttatcgtc
    47341 tggaaacagt tcggctggct gggtctgtac gaaattattc cgggctttat cttcggcagt
    47401 atcgggattg tagtgtttag tttgctgggt aaagcgccgt cagcggcgat gcaaaaacgc
    47461 tttgccgagg ccgatgcgca ctatcattcg gctccgccgt cacggttgca ggaagggtaa
    47521 gggacttagc ctgcggcggt tttgtttggc ttcagcagcg ggttgcgctc ccttaatgtg
    47581 cctcgccata taaattgaat ggtgcaggga gcgcgcaggg ggcggc
//
LOCUS       DAZKGF010000014        46611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZKGF010000014 DAZKGF010000000
VERSION     DAZKGF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.014"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            13..2439
                     /gene="ynfE"
                     /locus_tag="ACHOQV_000804"
     CDS             13..2439
                     /gene="ynfE"
                     /locus_tag="ACHOQV_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFP9629218.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLVNENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            2500..4923
                     /gene="ynfF"
                     /locus_tag="ACHOQV_000805"
     CDS             2500..4923
                     /gene="ynfF"
                     /locus_tag="ACHOQV_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFP9629219.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAETPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLATIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            4934..5551
                     /gene="ynfG"
                     /locus_tag="ACHOQV_000806"
     CDS             4934..5551
                     /gene="ynfG"
                     /locus_tag="ACHOQV_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFP9629220.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            5553..6407
                     /gene="ynfH"
                     /locus_tag="ACHOQV_000807"
     CDS             5553..6407
                     /gene="ynfH"
                     /locus_tag="ACHOQV_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFP9629221.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAANNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWYNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCTGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            6450..7064
                     /gene="dmsD"
                     /locus_tag="ACHOQV_000808"
     CDS             6450..7064
                     /gene="dmsD"
                     /locus_tag="ACHOQV_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFP9629222.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            7223..8515
                     /gene="clcB"
                     /locus_tag="ACHOQV_000809"
     CDS             7223..8515
                     /gene="clcB"
                     /locus_tag="ACHOQV_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFP9629223.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(8468..9163)
                     /gene="bioD"
                     /locus_tag="ACHOQV_000810"
     CDS             complement(8468..9163)
                     /gene="bioD"
                     /locus_tag="ACHOQV_000810"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP9629224.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(9288..10508)
                     /gene="mlc"
                     /locus_tag="ACHOQV_000811"
                     /gene_synonym="dgsA"
     CDS             complement(9288..10508)
                     /gene="mlc"
                     /locus_tag="ACHOQV_000811"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFP9629225.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(10643..11536)
                     /gene="ynfL"
                     /locus_tag="ACHOQV_000812"
     CDS             complement(10643..11536)
                     /gene="ynfL"
                     /locus_tag="ACHOQV_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9629226.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            11643..12896
                     /gene="ynfM"
                     /locus_tag="ACHOQV_000813"
     CDS             11643..12896
                     /gene="ynfM"
                     /locus_tag="ACHOQV_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9629227.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYVGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            complement(12946..13065)
                     /locus_tag="ACHOQV_000814"
     CDS             complement(12946..13065)
                     /locus_tag="ACHOQV_000814"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFP9629228.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            13321..13629
                     /gene="asr"
                     /locus_tag="ACHOQV_000815"
     CDS             13321..13629
                     /gene="asr"
                     /locus_tag="ACHOQV_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFP9629229.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            13722..13805
                     /locus_tag="ACHOQV_000816"
     CDS             13722..13805
                     /locus_tag="ACHOQV_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629230.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            13905..14726
                     /gene="ydgD"
                     /locus_tag="ACHOQV_000817"
     CDS             13905..14726
                     /gene="ydgD"
                     /locus_tag="ACHOQV_000817"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFP9629231.1"
                     /translation="MRRTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKTDKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(14765..15094)
                     /gene="mdtI"
                     /locus_tag="ACHOQV_000818"
     CDS             complement(14765..15094)
                     /gene="mdtI"
                     /locus_tag="ACHOQV_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFP9629232.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(15081..15446)
                     /gene="mdtJ"
                     /locus_tag="ACHOQV_000819"
     CDS             complement(15081..15446)
                     /gene="mdtJ"
                     /locus_tag="ACHOQV_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFP9629233.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            15857..16891
                     /gene="tqsA"
                     /locus_tag="ACHOQV_000820"
     CDS             15857..16891
                     /gene="tqsA"
                     /locus_tag="ACHOQV_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFP9629234.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLQQYRNSIM
                     TPLKAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(16916..18304)
                     /gene="pntB"
                     /locus_tag="ACHOQV_000821"
     CDS             complement(16916..18304)
                     /gene="pntB"
                     /locus_tag="ACHOQV_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFP9629235.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATILGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(18315..19847)
                     /gene="pntA"
                     /locus_tag="ACHOQV_000822"
     CDS             complement(18315..19847)
                     /gene="pntA"
                     /locus_tag="ACHOQV_000822"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFP9629236.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFTAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            20371..21315
                     /gene="ydgH"
                     /locus_tag="ACHOQV_000823"
     CDS             20371..21315
                     /gene="ydgH"
                     /locus_tag="ACHOQV_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFP9629237.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            21501..22883
                     /gene="ydgI"
                     /locus_tag="ACHOQV_000824"
     CDS             21501..22883
                     /gene="ydgI"
                     /locus_tag="ACHOQV_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9629238.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWS
                     ITGAGILLLTFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            22920..23642
                     /gene="folM"
                     /locus_tag="ACHOQV_000825"
     CDS             22920..23642
                     /gene="folM"
                     /locus_tag="ACHOQV_000825"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFP9629239.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIISYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(23639..23974)
                     /gene="ydgC"
                     /locus_tag="ACHOQV_000826"
     CDS             complement(23639..23974)
                     /gene="ydgC"
                     /locus_tag="ACHOQV_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFP9629240.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            24103..24822
                     /gene="rstA"
                     /locus_tag="ACHOQV_000827"
     CDS             24103..24822
                     /gene="rstA"
                     /locus_tag="ACHOQV_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFP9629241.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDRAEETILQE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLSKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            24826..26127
                     /gene="rstB"
                     /locus_tag="ACHOQV_000828"
     CDS             24826..26127
                     /gene="rstB"
                     /locus_tag="ACHOQV_000828"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFP9629242.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAVESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            26203..27132
                     /gene="tus"
                     /locus_tag="ACHOQV_000829"
     CDS             26203..27132
                     /gene="tus"
                     /locus_tag="ACHOQV_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFP9629243.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAELEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKTAVRLPGVLCYQV
                     DNLSQAALVSHIQHINQLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IATLPQDAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(27129..28532)
                     /gene="fumC"
                     /locus_tag="ACHOQV_000830"
     CDS             complement(27129..28532)
                     /gene="fumC"
                     /locus_tag="ACHOQV_000830"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFP9629244.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRVFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGG"
     gene            complement(28675..30321)
                     /gene="fumB"
                     /locus_tag="ACHOQV_000831"
     CDS             complement(28675..30321)
                     /gene="fumB"
                     /locus_tag="ACHOQV_000831"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9629245.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            30520..31695
                     /gene="manA"
                     /locus_tag="ACHOQV_000832"
     CDS             30520..31695
                     /gene="manA"
                     /locus_tag="ACHOQV_000832"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFP9629246.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISECYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVRGHGRLARVYNKL"
     gene            31796..33304
                     /gene="ydgA"
                     /locus_tag="ACHOQV_000833"
     CDS             31796..33304
                     /gene="ydgA"
                     /locus_tag="ACHOQV_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP9629247.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(33349..34614)
                     /gene="uidC"
                     /locus_tag="ACHOQV_000834"
     CDS             complement(33349..34614)
                     /gene="uidC"
                     /locus_tag="ACHOQV_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFP9629248.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQPADDEAGLRIRLKNELRRA
                     DKPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
     gene            complement(34653..36026)
                     /gene="uidB"
                     /locus_tag="ACHOQV_000835"
     CDS             complement(34653..36026)
                     /gene="uidB"
                     /locus_tag="ACHOQV_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFP9629249.1"
                     /translation="MNQQLSWRAIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LINDITS"
     gene            complement(36023..37834)
                     /gene="uidA"
                     /locus_tag="ACHOQV_000836"
     CDS             complement(36023..37834)
                     /gene="uidA"
                     /locus_tag="ACHOQV_000836"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP9629250.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESVLQEIRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(38221..38811)
                     /gene="uidR"
                     /locus_tag="ACHOQV_000837"
     CDS             complement(38221..38811)
                     /gene="uidR"
                     /locus_tag="ACHOQV_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFP9629251.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLSREASFAQGLRAMIGGILTAS"
     gene            complement(39032..39799)
                     /gene="hdhA"
                     /locus_tag="ACHOQV_000838"
     CDS             complement(39032..39799)
                     /gene="hdhA"
                     /locus_tag="ACHOQV_000838"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFP9629252.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(39911..40939)
                     /gene="malI"
                     /locus_tag="ACHOQV_000839"
     CDS             complement(39911..40939)
                     /gene="malI"
                     /locus_tag="ACHOQV_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFP9629253.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECSSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            41114..42706
                     /gene="malX"
                     /locus_tag="ACHOQV_000840"
     CDS             41114..42706
                     /gene="malX"
                     /locus_tag="ACHOQV_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFP9629254.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            42716..43888
                     /gene="malY"
                     /locus_tag="ACHOQV_000841"
     CDS             42716..43888
                     /gene="malY"
                     /locus_tag="ACHOQV_000841"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFP9629255.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFASAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQSVVYGPSVIYMVSE
                     LIRQWSEKGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKRADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            43992..44993
                     /gene="add"
                     /locus_tag="ACHOQV_000842"
     CDS             43992..44993
                     /gene="add"
                     /locus_tag="ACHOQV_000842"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP9629256.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(45029..46069)
                     /gene="ydgJ"
                     /locus_tag="ACHOQV_000843"
     CDS             complement(45029..46069)
                     /gene="ydgJ"
                     /locus_tag="ACHOQV_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9629257.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEGWGYDMRD
                     GVLTRVEGEERIEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            46312..46437
                     /gene="blr"
                     /locus_tag="ACHOQV_000844"
     CDS             46312..46437
                     /gene="blr"
                     /locus_tag="ACHOQV_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFP9629258.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
BASE COUNT        11441 a        12085 c        11435 g        11650 t
ORIGIN      
        1 agggagtgag ttatgtccaa aaatgaacaa atggtgggca tcagccgcag aacactcgtt
       61 aaatctaccg cgataggttc tctggcgctg gctgcaggcg gtttttcttt accgttcacc
      121 ctgcgcagtg cagcggcagc ggtacagcag gccagcgaga aagtggtctg gggtgcctgt
      181 tccgtcaact gtggtagccg ctgtgcactt cgtctgcatg ttaaagataa tgaagtgacc
      241 tgggtggaaa ctgacaacac cggcagcgat gagtatggca atcatcaagt acgcgcctgt
      301 ttgcgcggtc gctccatccg tcggcgtatt aatcatcccg accgcttgaa ttacccaatg
      361 aaacgcgtgg gcaaacgcgg cgaaggcaaa ttcgaacgga ttagctggga tgaagccctg
      421 gatactatcg ccagtagcct gaaaaaaacc gtcgaacaat atggcaatga ggctgtatat
      481 attcagtact cttcagggat cgttggcggc aatatgaccc gctcttcgcc atccgcctcg
      541 gcggtcaaac gcctgatgaa ctgctacggc ggttcactca accagtatgg ctcctacagc
      601 accgcgcaaa tctcctgtgc catgccctac acctacggca gtaatgatgg caacagcacc
      661 acggatattg aaaacagcaa gctggtcgtg atgtttggta acaacccggc tgaaacccgc
      721 atgagcggcg gtggcatcac ttatcttctt gaaaaagcgc gcgagaaatc gaacgcaaaa
      781 atgattgtta tcgatccgcg atataccgat acggctgctg gtcgtgaaga tgaatggctt
      841 cctattcgcc cgggcaccga tgccgcgctg gttgcgggta ttgcctgggt attggttaac
      901 gaaaatctcg ttgatcaacc ttttctcgat aaatactgcg tcggctatga cgaaaaaacc
      961 ttacccgcag atgcacccaa aaatggtcac tataaagcct atattcttgg tgaaggtgat
     1021 gataaaacag cgaaaacgcc gcagtgggct tcgcaaatta caggtatccc ggtagaccgt
     1081 atcatcaaac tggcgcgtga aattggcaca gcaaaacccg cttatatctg ccagggctgg
     1141 gggccgcaac gccaggcaaa cggcgagctg acggcacgcg ctattgctat gttgcctatt
     1201 ttgacgggca atgtcggcat cagcggcgga aatagtggtg cgcgtgaatc gacctacacc
     1261 attaccatcg aacgcctgcc ggtgctggat aatccggtca aaacgtcaat ctcctgcttc
     1321 agttggacag atgctatcga tcatggcccg caaatgacgg caatccgcga cggcgtccgg
     1381 ggcaaagata aactggatgt gcccattaag ttcatctgga actacgcggg aaataccctg
     1441 gttaatcagc attctgacat caacaaaacg catgaaattc tgcaggacga atcgaaatgc
     1501 gaaatgattg tggtcatcga aaactttatg acctcatcgg caaaatatgc cgacattctg
     1561 ctgccagacc tgatgaccgt tgagcaggaa gatattattc ctaacgacta cgccggtaac
     1621 atgggatatc tcattttcct ccagcctgtc accagcgaaa aattcgaacg caaaccaatt
     1681 tactggatcc tgagtgaagt cgcgaaacgt cttggacctg acgtctatca aaagttcact
     1741 gaaggtcgca cgcaggaaca atggttacaa catctgtacg ccaaaatgct tgccaaagat
     1801 ccggcgttac cgtcttacga cgaattaaaa aaaatgggta tctataagcg taaagatccc
     1861 aatggtcatt ttgtcgccta caaagcattt cgtgacgacc ccgaggcaaa tccacttaaa
     1921 acgccttccg gtaagattga aatttattcc agcaagctgg cggaaattgc ccgtacctgg
     1981 gaactggaaa aagatgaagt gataagccca ctgccggttt atgcctcaac ctttgaaggc
     2041 tgggactccc ctgaacgtag tacctttcca ctgcaactgt ttggtttcca ttacaaatcc
     2101 cgtactcact cgacctacgg caacattgat gtcctgaagg ctgcctgccg tcaggaggtg
     2161 tggatcaacc ctatagacgc acagaaacgt gggattgcca acggcgatat ggtgcgggtg
     2221 tataaccatc gtggcgaagt tcggctacca gccaaagtga caccacgcat tctccctggg
     2281 gttagcgcta tgggccaggg agcctggcac gaggccaata tgtctggcga taaaatcgac
     2341 catggcggct gtgtgaatac gctaaccact ctgcgccctt caccactggc gaagggaaac
     2401 ccgcagcaca ctaatctggt cgagatcgaa aaaatataac ccactacaac cataaattct
     2461 ggcatgacat tttgtttgaa aagcaataag tgagtaatga tgaaaatcca taccacagag
     2521 gcgctcatga aggctgaaat cagccgtaga agtctgatga aaacctccgc acttggcagt
     2581 ctcgcgctgg caagcagtgc tttcactctg ccattttccc aaatggtccg agcggcagag
     2641 acaccggtag aagagaaagc ggtctggagt tcctgcaccg ttaactgcgg gagccgctgt
     2701 ctgttacgtt tgcatgtgaa agatgacacc gtgtactggg tggagtctga tacgacagga
     2761 gacgacgttt acggtaatca tcaggttcgg gcatgtttac gcgggcgctc tatccgccga
     2821 cggatgaatc atcctgatag gttgaaatat cccatgaagc gcgtcggcaa gcgcggtgaa
     2881 ggtaaatttg aacggataag ttgggatgaa gccctggata ccatcagtga taatcttcgg
     2941 cggatcctta aagattacgg caatgaggct gtacatgtcc tgtacggaac aggcgtagat
     3001 ggcggaaaca tcaccaactc gaacgtcccg taccgtctga tgaactcttg cggtggtttt
     3061 ctcagtcgct atggcagcta cagtaccgct caaatcagtg ccgcaatgag ttatatgttc
     3121 ggtgccaatg atggcaacag cccggatgat atcgccaata cgaaactggt cgttatgttc
     3181 ggaaataacc cggcagaaac ccggatgagc ggcggtggtg tcacttacta cgtcgagcaa
     3241 gcccgagaac gttcaaacgc acgcatgatc gtcatcgatc cacgttataa cgacacagct
     3301 gccgggcgtg aagatgaatg gctgccaatt cgcccaggca ccgatggcgc actggcctgt
     3361 gcgattgcct gggtactgat tactgaaaac atggtcgatc agccatttct cgacaaatat
     3421 tgtgttggtt acgatgaaaa aacgctgccc gccaacgcac cacgtaacgc gcattataaa
     3481 gcctatattc tgggcgaagg gcctgacggc atagctaaaa cgccggaatg ggcagcaaaa
     3541 atcaccagca tcccggcaga aaaaattatc cagttggcac gagagatcgg ttcagcaaaa
     3601 cctgcttata tttgtcaggg ttgggggcca caacgacact ccaacggcga acaaacgtcc
     3661 cgcgctattg ccatgctttc cgttctcacc ggtaacgtcg gcataaacgg cggcaactca
     3721 ggtgtacgcg aaggtagctg ggatctgggg gtagaatggt tcccgatgct tgagaatcct
     3781 gttaaaacgc agatttccgt ctttacatgg acagatgcca tcgaccacgg tacggaaatg
     3841 accgcgaccc gtgatggtgt tcgtggaaaa gaaaaactgg atgtccccat caagttttta
     3901 tggtgctacg ccagtaacac attgatcaat caacatggcg acatcaatca cacccatgag
     3961 gtgcttcagg atgacagcaa gtgcgagatg attgttggca ttgaccactt catgactgcc
     4021 tcggctaagt attgcgatat cctgttgccc gacctgatgc caacagagca agaagacctt
     4081 atctctcacg aatctgccgg gaatatgggc tatgtgatcc tcgcccaacc cgcaacctca
     4141 gcaaaatttg aacgcaaacc catctactgg atgctgagcg aagttgccaa acgcttagga
     4201 ccagacgttt atcaaacctt tactgaaggc cgcagtcagc acgaatggat caaatatctc
     4261 catgcgaaaa cgaaggaacg taaccctgag atgcccgact acgaggagat gaaaacgacc
     4321 gggatcttta agaaaaaatg cccggaagaa cactacgtcg ctttccgcgc attccgtgaa
     4381 gatccacagg caaacccgtt gaaaacacct tcggggaaaa tcgaaattta ttctgaacga
     4441 ctggcgacga ttgcggatac ctgggaattg aaaaaagatg aaattattca tccccttcct
     4501 gcgtataccc caggttttga tggctgggac gatcccctgc ggaaaaccta tccactgcag
     4561 ctaacgggct tccactacaa agcgcgtacc cactccagct acggcaatat tgatgtgtta
     4621 cagcaggcct gcccacaaga ggtgtggatc aaccctattg atgctcaggc acgcggtatc
     4681 cgtcatggcg ataccgtgcg ggtatttaac aataatggag aaatgctgat tgccgcaaaa
     4741 gtgactccgc gtattctgcc tggcgttacc gccatcgggc aaggtgcgtg gcttaaagcg
     4801 gatatgtttg gtgaccgggt cgatcacggc ggcagtatca atattctgac ctctcaccgc
     4861 ccttcaccgc tggcaaaggg aaacccgtcg cacagcaatc ttgtccagat cgaaaaggtt
     4921 taaggagtag cccatgacca cacaatatgg attttttatc gattccagcc gctgtactgg
     4981 ctgtaaaacc tgcgaactgg cgtgcaaaga ttttaaagat cttggcccgg aagtcagttt
     5041 ccgccgtatt tacgaatatg ctggcggcga ctggcaggaa gataatggcg tctggcacca
     5101 aaacgtgttc gcttattatc tctccatctc ctgtaaccat tgcgatgacc ccgcatgtac
     5161 aaaagtctgc cccagcggcg caatgcataa gcgtgaagat ggctttgtgg tggttgatga
     5221 ggatgtttgt atcggctgcc gctactgcca catggcttgt ccgtacggcg cgccacagta
     5281 caatgctgaa aaagggcaca tgacgaagtg cgatggttgt tattcgcgcg tcgccgaggg
     5341 gaaacaaccc atatgtgtcg aatcctgccc gctgcgtgca cttgagttcg gtccaattga
     5401 agagctgcgc cagaagcacg gcactctggc tgccgtcgcg ccactaccgc gcgcgcattt
     5461 cacaaaaccc aatatcgtta tcaaacctaa cgccaacagc cgcccgaccg gcgataccac
     5521 tggttatctg gctaatccgg aggaagtgta aaatgggaaa tggatggcat gaatggccac
     5581 tggttatctt taccgtattg ggtcaatgcg tagtaggcgc gctgattgtt agcggaattg
     5641 gctggttcgc cgccaataat gacgcagacc gtcagcgtat tgtccgtggc atgtttttcc
     5701 tctggctgtt gatgggcatc gggtttattg cctctgtcat gcacctggga tcgccactgc
     5761 gagcgtttaa ctcgctgaat cgaatcggag cttctggtct gagtaacgag attgctgccg
     5821 gttccatatt ttttgccgtt ggcggattat ggtggctggt ggcagtcatc ggtaaaatgc
     5881 cgcaagcgtt ggggaaactc tggttacttg tcagcatggc gctcggcgtc attttcgtct
     5941 ggatgatgac ctgcgtgtat caaatcgaca cagtgccaac ctggtataac ggttacacta
     6001 cgctggcttt cttcctgaca gtattgttga gcggtccaat acttgctgcc acaattctgc
     6061 gggcagcccg cgttacattt aataccacac catttgccat cattagcgtt ctggcgctga
     6121 tcgcttgtgc cggggtgatt gtgctgcaag gtttgtctct ggcgtctatt cactcatccg
     6181 tacaacaagc cagcgcactg gtaccagatt acgcctcctt acaggtctgg cgtgtggtat
     6241 tgttatgcac aggtctagga tgctggcttt gtccgctcat tcgtcgccgt gaacctcacg
     6301 ttgccggact tattctgggg ctaatcctca tacttggcgg tgagatgatc gggcgtgtgc
     6361 tcttttatgg cctgcacatg acggtaggaa tggctattgc aggttaacaa attggtgcgg
     6421 ggcaacccgc acatttcagg atgttaggaa tgacccattt ttcacagcaa gataattttt
     6481 ctgtcgcggc gcgcgtgttg ggcgcgctgt tttattacgc tccagagagt gcggaagccg
     6541 cacctctggt tgcggtactc acccgtgacg gctgggaaac tcagtggcct ctaccagaag
     6601 cgtcattagc gccgctggtg acggcttttc agacacagtg tgaagagact cacgcccagg
     6661 cctggcagcg tttgtttgtc ggcccgtggg cactgccgtc tccgccgtgg ggatcggtct
     6721 ggctggatcg cgaatctgtg ctgtttggcg attcaacatt ggcacttcgt cagtggatgc
     6781 gcgagaaagg cattcagttt gaaatgaagc aaaacgaacc ggaagatcat tttggttcac
     6841 tgctgttgat ggctgcgtgg ttggcggaga atggtcgcca gacagaatgc gaagaattgc
     6901 tggcatggca cctttttccg tggtcaacac gatttcttga cgtttttatc gaaaaagcag
     6961 aacacccttt ctaccgtgca ctgggggaac tggctcgctt aacgctggcg caatggcaat
     7021 cacaactgtt gattcctgtc gcggttaaac cactctttcg ataggatcac cgtaatattg
     7081 ccggatggtg gcgattacgc gcaattcggc aataataggt tacagtgtca cgttttttta
     7141 tctcttaaag cacgcactgc ttttgcggct ggcctctttt gccgcaaaat agtcgcccgt
     7201 gtttcattgc ccatttctgc tcatgcatca tctacacatc tatccggatc tgcgcactat
     7261 gttccgccgt ctgcttatcg caacagtcgt cggtattctt gcggcctttg ccgttgccgg
     7321 gtttcgtcat gcaatgctgc tactggagtg gttgttcctc aataatgact cgggcagtct
     7381 ggtcaatgca gcgacaaatc tttccccatg gcgacggctg ctaactccgg cgctcggcgg
     7441 actggcggcg ggtttgttgc tgatgggctg gcagaaattt acccagcaac gccctcatgc
     7501 gccgaccgat tacatggaag cgttgcaaac cgatgggcag tttgattacg cagcaagcct
     7561 ggttaaatcg cttgcctctc tgctggtagt aaccagcggc agtgcaattg gtcgcgaagg
     7621 tgcgatgatt cttttagctg cccttgccgc ctcctgtttt gcccaacgtt ttacgccacg
     7681 ccaggagtgg aaattatgga tcgcctgtgg agctgcggcg ggaatggctg cggcctatcg
     7741 cgccccgctt gctggcagtt tatttatagc cgaagtgctg tttggcacta tgatgttggc
     7801 ctctctcggc ccggtgatta tttccgccgt cgtggcgttg ctggttagca atctgattaa
     7861 tcatagcgac gcgttactct acagcgtaca actctcagtg acggttcagg ctcgtgacta
     7921 tgcgctgatt atcagtacag gtgtgctggc aggtctgtgc ggaccactgt tgttaacgtt
     7981 aatgaacgcc tgtcatcgtg gatttgtaag tctcaaactt gcgccgccct ggcaactggc
     8041 actgggcggg ttgatcgtgg gtctgctttc cctgttcaca cctgcagtgt ggggcaacgg
     8101 ctatagcacc gtacaatcct ttttaaccgc cccgccactg ttaatgatca ttgccgggat
     8161 cttcctttgt aaactgtgtg ccgtgctggc gagtagcggt tccggcgcac ccggtggtgt
     8221 ctttacaccg acgctattta tcggtcttgc cattggcatg ttgtatggtc gtagcctggg
     8281 attatggttc cctgatggcg aagaaattac gcttttactc ggattgaccg ggatggcgac
     8341 actgttggcg gcaaccacgc acgcgccgat tatgtcgacg ttgatgatat gtgaaatgac
     8401 cggggagtat cagctactcc ccggtttatt gattgcctgc gtaattgcgt cggtaatttc
     8461 gcggacgtta caccgtgact ctatctaccg ccagcacact gcgaagcata gctaagcgga
     8521 tgtattgccc cagttcacgc tgttcggcgc gcggcagata aggcagttca ccaatgagcg
     8581 gtgccggaag ttttttaccg agcacatcaa tgatttccgc ataatgcgcc agtcctggat
     8641 tgattcggtt agccacccag ccaatgagcg gcagcccgtc gttggcaatc gcctgagctg
     8701 ttagtagtgc atggttaatg caaccttcct gaataccgac aaccatcaat accggcagtt
     8761 gttcctgcac cacccattca gagagcggac gcaaatcatt catcagactg cgccagccgc
     8821 cagtcccttc taccacgaca tgatcgactt tatcggtcag gtttgccagg ccgtttgaaa
     8881 tgagggtgta attgattggg caactgtgcg ccacgctact ttcttcttcg cttaacgcga
     8941 taggattaac tgcttcataa ggcagttcga tggttgaaac actctgcaac accagggcat
     9001 ctttattacg cagcccttcg ggtgtctctt tgctcccctt cgctacgggt ttatatcccg
     9061 caaccgtttt tccctgggag gctaacgctt gtagcaatgc gcgggaaacc accgttttcc
     9121 ctacagaagt gtctgtaccg gtaataaaga aacgcttcag catcactaac tccaccgtta
     9181 tgcttcacaa atataaacca ggaaaataat taaccttgaa agtctaagtt atgctttcct
     9241 ggcccaaatt gagatagcgc aaattttggt agaacagtta aaaaatgtta accctgcaac
     9301 agacgaatca acaaagaacc gttatacatc gcgtctttta ccagcgcagc ccctgccatc
     9361 gtaccctggt tagaaaactg agtactctca acgctaatgt gctgactata cgcaggaagg
     9421 gcctgctgac ggatgctgtc cgagatgacc gggaagagga tatctgccgc tttacttaac
     9481 ggtgagccaa tcagtatttt ttgtgggtta aataaattca ccatgatggc aagaatgcgc
     9541 cctacatgcg cgcccacccc ggtaatgatg tcttttgcca gtagatcgcc gcgcaatgcc
     9601 gcctgacaca atgaatccac ggttaacggc tgtccatgta acatcgagct catggattga
     9661 ttgagacgca gctgtgccag ctcaagaata ctgtccacac tggcgatggt ttcgaggcag
     9721 ccgtgattcc cgcaataaca gcgtttccca tacgggtcga cctgcgtgtg acctatttcc
     9781 acgaggctac tgctgcctgc gtgtagcaga tgaccatcgg taatgacgcc cgcccccacg
     9841 ttgtgatcga taaccacctg aatcacatcg cgcgccccgc gtgaggcacc aaacaaggcc
     9901 tcagccatcg tccatgcgct gatatcatgc tgaatataaa ccggtacgcc ggtatgctgc
     9961 tccagcgcct cgccgagcgg catctctttt acatcctcgt agaacggcat gcgatgtaca
    10021 ataccatttt ccgtatcaat aattcccggc aaggttatgg caatcgaagt cagacgctca
    10081 agttttttct ggtggcggat aaaaaactga tcgatatggg aaatgatacg atccagcaat
    10141 ggcgagtcat cttttaacgc cagttcctgc gactcctcca ctaccagttt gctgctcaga
    10201 tcgcgcaaag caaggaaaat ctccccgcga ctaatgcgca gagaaagata gtgccaggct
    10261 tcagtttcaa ccaccagccc cactgccgga cggccacggt tccccgcttc tttgatttcc
    10321 agctcttgca ccaggtgtgc ttcgagcatc tcacggacaa ttttagtgat actggcagga
    10381 gccagctgcg ccagacggga aagatcgata cgcgagactg gaccaagctg atcaatcagg
    10441 cgataaaccg cgcccgcgtt ggtctgcttt atttgatcaa tgtgcccagg ctgattttca
    10501 gcaaccaccg catactccct atattttcgc gctccgaaat aatctgtagg ctatggtgaa
    10561 gcacttcaat acgtgtcgtc aaatttttac ttaggcatgt gattaacagc acatttttcg
    10621 ggctttttcg ctgaaatttc cctcacctga gagcattcag cagatgaata cgaaagttac
    10681 gcgcagccgg actttgttca tggtgtttcg gccagaccaa ccacatttca gaaaccgcat
    10741 cctcttcagc aatcggcacc cagcgcattt cgttgagctg aacacgttta aatgacgcag
    10801 gcaaaattga aacacccaga ccagcggaaa ccagaccgat gatggtcatt gcctcaccca
    10861 cctcctgagt gatgacgggc gtcaaattgt aacgtcgcat cagcccgaga atatcgtcat
    10921 acagccctgt ccctacgtgc ggatcaaaaa agacaaaggg ttctttcgcc agttcagcca
    10981 gcgttacact cgggttattt gccaagggat gatcgtgcgg gatcatcgcc ataagcggtt
    11041 catggacgat gactgcgtgt tcaagcgtct ccggtaacgc tgtgttacgc agcaatccca
    11101 tatccagcgt tccttcaatg agcggagcga tttgctcgcg ggtgttcatt tcgcgggttt
    11161 gtaaatggac atcgggataa tcacggcgaa acagcgataa cgtatcggac accgcccgaa
    11221 taaaaggagc ggacgaagta aaaccaatgc gcaactcccc cgcttcaccc tgatgcagtc
    11281 tttcagcgcg agcggcagcg tcatccacca tagacaggat ttgccgacta tctgcaagaa
    11341 actgttttcc tgctgccgtc agcaatacac tgcgattagt tcgtgccagc agtcgggcac
    11401 caatttgttg ctccagcgcc tgaatctgct gacttagcgg cggttgcgaa atattcaggc
    11461 gggcagcggc gcgcccgaaa tgcagctctt ctgcaacagc aacaaagtaa cgcagatgac
    11521 gaagttcaat attcatattt aaaacatctt atttgagatt attaatatat tagacagaac
    11581 aattcgattt tcctaatcta ttcataagcc tgaaccacag gcatatttag caaggatttc
    11641 aagtgagccg tactacaact gttgatggcg ctccggcaag cgacactgac aagcaaagca
    11701 tttctcagcc aaatcaattt attaaacgcg gtacgccgca atttatgcgc gtcaccctgg
    11761 cgctgttctc tgccggactg gcaaccttcg cacttctcta ttgtgtgcaa cctatccttc
    11821 cggtgctttc gcaggagttt ggcttaacgc ccgcgaacag tagtatttca ctgtccattt
    11881 ctacggcgat gttggctatc ggtttgctgt ttactggccc gttatccgat gccattggtc
    11941 gcaaaccagt gatggtcacg gcgctactgt tggcctccat ttgtacgtta ctttcgacaa
    12001 tgatgaccag ctggcacggc attttgatta tgcgcgcctt gattgggctt tcgttaagtg
    12061 gcgtagcagc tgttggcatg acttatctta gcgaggaaat ccaccccagt ttcgtggcct
    12121 tttcgatggg gttgtatatc agcggcaact caattggcgg catgagcgga cgcttaatta
    12181 gcggtgtctt cacggacttt ttcaactggc gaattgcgct ggcggcaatc ggttgtttcg
    12241 cgctggcctc ggcgttaatg ttctggaaaa tcctccctga atcacgccat tttcgcccga
    12301 cttcactgcg ccctaagacg ttattcatca actttcgtct gcactggcgt gaccggggat
    12361 taccgttatt gttcgcagaa ggctttttgc tgatggggtc gttcgtcacg ctgtttaatt
    12421 acgtcggcta tcggttgatg ctctccccct ggcatgtcag ccaggccgtg gttggcttat
    12481 tatcgctggc ttatttgacc ggtacatgga gctcacccaa agccggaacc atgaccaccc
    12541 gctatgggcg cggtccggtg atgttgtttt cgacgggggt tatgctgttt ggtttattga
    12601 tgaccttatt cagctcgctg tggctgatct ttgccggaat gttactcttc tcagccggat
    12661 tcttcgcagc ccactcagta gccagcagct ggatcggccc ccgcgcaaaa cgcgctaaag
    12721 gccaggcctc ctcgctgtat ctgttcagtt actatctggg gtcgagtatt gccgggacgc
    12781 tgggtggtgt tttctggcat aactatggct ggaacggcgt cggcgcattt attgctctga
    12841 tgctggtcat tgctctgctg gtcgggacgc gtttgcatca tcgtctgcac gcctgaaaaa
    12901 ataagtccgg actgaggtaa atacccgtcc ggacttattg cctgctcaaa ccaacgttaa
    12961 tagccatcct aaaatagacg aagcgccagc caattcccgc agcgcatcta gtgtcatcag
    13021 gattataagt acccaaataa acggattcat tttgctgtgt gtcatttatt actgatgcgc
    13081 agttattcta ccgctttgta agtagtaaaa tagttaacac gatcaagact actattattg
    13141 gtagatgaat tttccttaag tcacaatacg ttattatcaa cgctgtaatt tattcagcgt
    13201 ttgtacatat cgttacacgc tgaaaccaac cactcacgga agtctgccat tctcagggat
    13261 atagttattt caacggcccc gcagtggggt taaatgaaaa aacaaattga gggtatgaca
    13321 atgaaaaaag tattagctct ggttgttgcc gctgctatgg gtctgtcttc tgctgccttt
    13381 gctgcagaga ctgcgaccac acctgctccg actgcgacga ccaccaaagc agcgcctgcg
    13441 aaaaccacac atcataaaaa acagcataaa gcagcacctg cccagaaagc gcaggcggct
    13501 aaaaagcatc ataaaaatgc caaagctgaa cagaaagctc ctgaacaaaa agcgcaggca
    13561 gcgaagaaac acgccaagaa acacagccat cagcaaccgg caaaacctgc tgcacaaccc
    13621 gcagcgtaag ttttcaacag taatgctggc gcgccccctc gcgcctgaaa attacggtgc
    13681 taaacgggta acgtttagca ccgcctttag cggagggtag tatggtgggc cgttatcgct
    13741 ttgagttcat tcttatcatc cttattttat gcgcactgat tgccgcccgt ttttatcttt
    13801 cctgatcgta gttatctgat tttactccca ctttcatccc gtcccgtcta tagtatttat
    13861 gagggtttgc ttttaataat cataattacc caccagagtg tgatatgcgt agaaccattg
    13921 ctgtagtgtt gggtgcaatt agtttgacgt ctgcttttgt gtttgcagat aaaccagacg
    13981 ttgccaaatc ggcaaacgat gaggtcagca ccctgttttt tggtcatgat gatcgtgtgc
    14041 cagtgaatga cacgacccaa tcaccgtggg atgcggttgg ccaactggaa acggccagcg
    14101 gcaatttatg tacagcgacg ctgattgcac ccaatctggc attaacggca ggacactgtt
    14161 tattgacgcc gccaaagggt aaaaccgata aagcggtggc gctgcgtttt gtgtcaaata
    14221 aaggtctttg gcgctatgag atccacgaca tagaaggccg cgttgacccg acactgggaa
    14281 agcggttaaa agcagatggg gatggttgga ttgttcctcc cgcagctgcg ccgtgggact
    14341 tcggtttgat tgtgctacgt aatccccctt ctggcattac accattgccg ttatttgagg
    14401 gagataaagc cgcgcttacc gccgcattaa aagcggcagg tcgtaaagtg actcaggcgg
    14461 gataccctga agatcatctc gatacgttgt acagtcatca aaactgtgaa gtgactggct
    14521 gggcgcaaac gtcggtgatg tcgcatcagt gcgatacctt gccgggtgac agcggttcgc
    14581 ctctgatgtt gcataccgat gacggctggc aattaattgg ggtgcaaagt tcggctcctg
    14641 ccgcgaaaga tcgctggcgc gccgataacc gggccatttc tgttaccggt tttcgcgaca
    14701 agctggatca actgtcgcaa aaataatgtt caaacgctgc ccgacagcgc gggcagcatg
    14761 ttcatcaggc aagtttcacc atgatcattc cagccaacag cagcaccagg ccaatccagc
    14821 ctttacgatt taaccgttga ccaaacaaga tccaacctgc ggctaacgtg gcggcaatac
    14881 caaacccgcc ccacaatgca taagcgacag acaagtcgat ccctttaacg gcctgagaaa
    14941 gcgcactaaa ggcagccagc accgccgcca gagagagcaa gccaaatatt ttgcgacgaa
    15001 agccgtcaga aaatttcaaa aagacgttag caacgatttc cagcacgatt gccaatgcca
    15061 gccaggcggc gtgaacccat tcaaactgcg ccatggttca cctccagttc aggtttacgc
    15121 gctttacggg tacctgattt gatcaacaca atcccggcga ccagggtggt taacccggca
    15181 attttcatca gagataaact ttcgtcgaat aacaaaacgc taaacaaggt aataaataaa
    15241 ataccgatac cttcccacag cgcataagct acgcctaagg cgatcttttt aacggcgaaa
    15301 gagagaaata tatacgacag agaaatcatc accagcatta aaataaagcc gccatttccc
    15361 tcactgacgc tcgcccattt catggacagc gtaccggtaa tttctgtagc aatagccaga
    15421 cctaataaaa tccaataaat atacattgtc cttctcctgc aagagaatta ttttaatttt
    15481 cgcttaattc agcgaaacca aagtaaagtg gtcaagcccg cgcttagcgc aagttttggc
    15541 agaagaaagg actaaagcgc gttgcgccag tgctgctcac ctacgagcaa gatagtggat
    15601 gaggtacgaa gatgggtaaa tgtagaaaat aacgtcctga acaaattgtc cataatatta
    15661 caattatccg cagtgttgct tctcgtcatc gcggatgata attgtcctcg gtagttgaac
    15721 acgcctgatt tgtatcatag cttaagaatt aactcaaaat attttcactt ctttacctga
    15781 gcggtttgat tttcgttatg atgacggagc gaaaaagaca ttattattag caaaggaaga
    15841 aaaaacgggg acaagcatgg caaagccgat catcacgctc aatggcctaa aaatcgtcat
    15901 tatgttggga atgctggtca ttattctctg cggtatccgt tttgccgccg agatcatcgt
    15961 gccgtttatt ctcgcattat ttattgctgt tattcttaac ccgctggtgc aacacatggt
    16021 ccgctggcgt gtgccgcgtg tactggcagt gtcaattttg atgaccatca tcgtgatggc
    16081 gatggtgttg ctgttagctt atctgggttc cgcgctcaat gagttgacgc ggacgttaca
    16141 gcaatatcgc aactctatta tgacgccact gaaagcgatt gaaccgttgt tgcaacgcgt
    16201 agggattgat gtctcagttg accaactggc gcattacatt gatccgaacg cggcgatgac
    16261 gttgctcacc aacttattga cgcagttatc taatgccatg tcatcaatct ttttattgct
    16321 gctgacggtg ctgtttatgc tgctcgaagt gccacaattg cccggaaaat ttcagcaaat
    16381 gatggcgcgt ccggttgaag ggatggcggc gattcagcgt gcgattgaca gtgtgtctca
    16441 ttatctggtg ctgaaaacag ccatcagcat catcaccggc ctggtcgcat gggcgatgct
    16501 cgccgcactc gatgttcgct tcgcttttgt ctggggattg ctggcctttg cgcttaatta
    16561 catcccgaat attggttcag ttctcgcggc aatcccccct attgctcagg tactggtgtt
    16621 taatggcttc tacgaagcgt tgctggtgct ggcgggatat ctgctgatta atctggtctt
    16681 cggcaatatt ctggagccgc gcatcatggg acgtgggctg gggctttcga cattggtggt
    16741 atttttgtcg ttgatttttt ggggatggtt gttaggaccg gtgggtatgc tgctttccgt
    16801 gccgttgaca attattgtca aaattgcgct tgaacaaaca gcgggaggtc aaagcatcgc
    16861 cgttctgtta agcgatctca ataaagagtg acggcctccg cagaggccgt cagggttaca
    16921 gagctttcag gattgcatcc acgctggctt tggcgtcacc aaacagcatg tgggtgtttt
    16981 ccttgaagaa cagcgggttt tgcacaccag catagccagt gttcatcgaa cgtttaaaga
    17041 caatcacgtt ctgcgctttc cacacttcca gcacaggcat accagcaata ggactcttcg
    17101 gatcatcctg cgccgccggg ttaaccgtat cgttagcacc aatcaccagt acggtatcgg
    17161 tatcagcaaa gtcatcgttg atctcgtcca tttccagcac gatgtcatac ggtacttttg
    17221 cttcagccag caatacgttc atatgtccag gcaaacgccc cgcaaccggg tggataccga
    17281 aacgcacgtt gataccacga gcgcgcagtt tctcggtaat ttcagcgaca ggatattgcg
    17341 cctgcgcgac tgccatgccg taccccggag taatgatcac tgaatgggag tttttcagca
    17401 gttccgctgt ctcttctgcg gtgatttcgc ggtgctcgcc cacttcctga tcatcgccag
    17461 tagaagagcc gtcggtgccg aaaccacccg caataacgct gataaaggaa cggttcatcg
    17521 ccttacacat aatgtaagaa aggatagcgc ccgaagaacc gaccagcgca ccggtcacaa
    17581 tcagcagatc gttgctgagc ataaagcccg cagccgcagc cgcccagccg gagtacgagt
    17641 tcagcatcga caccaccact ggcatatctg caccaccgat ggaggcgact aaatgccagc
    17701 cgaataccag cgcaatggcg gtcattatca gcaatgccag cacttgcagg ccgacgctgt
    17761 ccgtgcgaac aaatacaatc agcagcagga aggaaacgac cagagccgcc aggttcattt
    17821 tgtgacggtt tggcagcatc aacggtttag acgaaatctt gccacacagt ttgccgaacg
    17881 ccaccaccga acccgtgaac gttaccgccc cgatgaagat accgaggaac acttccgtca
    17941 ggtgaatatt gaccagaatc ggtgccattc ccgcgtcatg atgcagatag ctgttaaagc
    18001 caaccagcac tgccgccaga cccacgaagc tgtgcaggat cgccaccagt tctggcattt
    18061 cggtcatttc aactttcttc gccagccgga taccaattgc cccaccaatg accatcgcca
    18121 gcaagatcca gccaacatta cccgtatccg gtcccaaaat ggttgcgatt aacgcaatcg
    18181 ccatcccggc gataccgaag ttgttaccct ggcgagacgt ttcatgtttc gaaagaccgg
    18241 ccagactgaa gataaacagg atcgcggcaa caatgtatgc agctgtaact aatcctccag
    18301 acatatgtta ccccttaatt tttgcggaac attttcagca tgcgctgagt cacggtgaag
    18361 ccaccgaaaa tattaatgct ggctataagc accgcgataa aactaaggaa gctaacccag
    18421 ccgccctggc caatctgcaa cagtgctccg acaacaataa tccctgaaat cgcgttggtg
    18481 accgacatca acggtgtatg cagcgcgtgc gatacattcc acaccacgta ataaccgaca
    18541 acacaggcca gcgcaaaaac ggtgaagtgc ccaagaaact ctttcggcgc aacgcttgcc
    18601 aaccagccga aaagaatgat tgccagcgcc atcaacgcgt atttacgcca cggtgagcag
    18661 gcacattttt cctcggtttt cacttccggt gccgcttttt gtgccgcctg cggctgagct
    18721 gatacctgaa tcggcggtgc cggccaggta atttcgcccg cacggatcac ggtcacgccg
    18781 cgaatcacca catcatcaaa atcaacagtg atattgccgt ctttctcttt gcacaacagt
    18841 ttcagcagat taacgaggtt agtaccgtaa agctgtgagg attgcgtcgg cagacggccc
    18901 ggaagatcgg tataaccaat cactttgaca ccattttccg tagtgaagat ttcacccggc
    18961 acggtgtatt cacagttgcc gccgttttgg gctgccaggt cgacaatcac actacccgcc
    19021 ttcatggagt caaccatttc acgggtaatt agcttcggcg ctggtttgcc tggaataagc
    19081 gcggtggtga caatgatatc gacctctttt gcctgggcgg taaagagttc catttccgct
    19141 ttgatgaacg cgtccgacat cactttggca tagccatcgc cgctgcccgc ttcctcttta
    19201 aaatccagct cgaggaattc cgcgcccata ctttgaactt gttctttcac ttccgggcgg
    19261 gtgtcgaatg cacgcacaat cgcgccgaga ctgtttgcag cgccaatggc ggccagaccc
    19321 gcaacacccg caccaatcac catcactttt gccggtggca ctttcccggc cgcagtaatt
    19381 tgcccggtaa agaagcgccc aaattcatgt gccgcttcaa caatggcgcg ataaccggcg
    19441 atattcgcca tcgagcttag tgcgtccagc gattgtgcgc gtgagatacg cggcacagag
    19501 tccatcgcca tcacggtcac gttacgttcc gcaagttttt gcattaattc cggattctgc
    19561 gcaggccaga taaaactcac cagcgttgtc ccaggattca gtaacgcaat ttcatcatct
    19621 aacggcgcat tgaccttcag aatgatctct gactgccaga cgctattccc ttctacaatt
    19681 tcagcgcccg cttgcacaaa cgctttatcg tcaaaacttg ccagttgacc cgcgccgctc
    19741 tctaccgcga cggtaaaacc cagtttcagc agctgttcca ctgtttttgg cgttgctgca
    19801 acacgggttt cattggttaa ccgttctctt ggtatgccaa ttcgcatgat attcccttcc
    19861 atcggtttta ttgatgatgg tttgcctgtg tcaggagcca cacaagccgc tcatgtacga
    19921 gctaaatgtt actccgttaa aataaattag taacaaacgc cctataacgt actgaaaatt
    19981 atgcctgtga tctagcgcca aaaatcagta tttcggcgtg aactcgcaaa atattaacga
    20041 ttcagccgtg atagtgggat aaacacctta gaacgccgga taaagactga taattgtctt
    20101 cggcgttcgc ataaaacgag acatcgcccc ggcacgaatc actacttaac attaaattaa
    20161 cttatacaat tcagttgctt cagtagtatt gatgctgata cggctgtttt ttaagcatag
    20221 acggccattt gagcaggatt aaaattggct taaggaatgt gatatgaaaa atgacgcaga
    20281 cagttacacc gtttaaatgc aataatcagc cacgtttctc gttaataaca ataccagtac
    20341 ctggtttgcg caaggcgaag gattattttt atgaagctta agaacaccct cctggcgtcg
    20401 gcactgcttt ctgctacggc attctccgtt aacgcagcaa cagaactgac accggagcaa
    20461 gcggcagcgg ttaaaccttt tgaccgtgta gtggttaccg gtcgttttaa tgctattggc
    20521 gaagcggtga aagccgtttc tcgtcgcgct gataaagaag gtgccgcctc tttttatgtt
    20581 gtcgacactt ctgattttgg taacagcggt aactggcgtg tggtcgctga cctctataaa
    20641 gccgatgctg aaaaagcaga agaaacaagt aatcgcgtaa ttaacggtgt tgtcgaactg
    20701 ccgaaagatc aggctgttct gattgaaccg tttgacacgg tcaccgtcca gggcttctat
    20761 cgtagccagc cagaagtcaa tgatgccatc accaaagcgg caaaagcgaa aggtgcctac
    20821 tctttctaca tcgttcgtca aatcgatgcc aaccagggcg gcaaccagcg tattactgca
    20881 ttcatctata aaaaagatgc taagaaacgt atcgtccaga gcccggatgt gatcccggca
    20941 gattccgaag caggacgtgc agctctggct gccggtggcg aagctgcgaa gaaagttgag
    21001 atcccgggtg ttgcgactac cgcatcacca agttctgaag tcggtcgctt ctttgaaacc
    21061 cagtcatcaa aaggcgggcg ttacactgtc acgctcccag acggcactaa agtcgaagaa
    21121 ctgaacaaag cgaccgcagc gatgatggtc ccgttcgaca gcatcaaatt ctctggcaac
    21181 tacggcaaca tgactgaagt ctcctatcag gttgcgaaac gtgccgcgaa gaaaggtgct
    21241 aagtattacc acatcacccg ccagtggcaa gaacgtggta ataacctgac cgtcagcgca
    21301 gatctgtata aataactatt cagttactcc tataaggcgg cttgatgccg cctttcgcat
    21361 ttttagtcac aaatttcccc gcgaccattg catgcagaca catctcttcg taaaatcccg
    21421 cgcttcagtg gcaaactcca tttttatgcg ttttgcccta atatttattc ttcttgtcac
    21481 gttttaatca ctggatatcg atggaaaaga aactgggact gagcgcactc accgcgctgg
    21541 tattaagctc aatgctgggc gcgggtgttt tcagtctgcc gcaaaatatg gcggcagttg
    21601 ccagcccggc agcactgctc atcggctgga gtattactgg tgctggtatt ttattactga
    21661 cctttgccat gctgatcctc acgcgcattc gtcccgaact tgacggcggt atctttacct
    21721 atgcccgcga aggatttggt gagctaatcg gcttttgttc cgcatgggga tactggctgt
    21781 gcgcggtcat cgccaacgtc tcctatctgg tgatcgtttt ttccgcgtta agctttttta
    21841 cggacacgcc ggaattgcgc ctgtttggcg atggtaatac ctggcaatcg atcgtcggtg
    21901 catcggcttt attgtggatt gttcactttt tgatcctgcg cggagtgcaa accgctgcca
    21961 gcattaacct ggtggcgaca ctggcaaaat tgttgccgct gggtctgttt gttgtgctgg
    22021 cgatgatgat gttcaaactg gataccttca agctcgactt caccggactt gcacttggcg
    22081 tacccgtttg ggaacaggtg aaaaacacca tgctgatcac cctgtgggtt ttcattggtg
    22141 tggaaggtgc cgtggttgtt tctgcgcgtg cgcgtaataa acgtgatgtt ggcaaagcga
    22201 cactgctggc ggttctctcc gctctgggcg tttacttgct ggtaacgctg ctttcgctgg
    22261 gcgtggtggc acgtcctgaa ctggctgaaa ttcgtaaccc gtcaatggcc ggactgatgg
    22321 tggaaatgat gggaccgtgg ggcgaaatca tcatcgctgc cggtttgatc gtttccgttt
    22381 gcggtgcgta cttaagctgg accatcatgg cggcagaagt tccgttcctg gcagccactc
    22441 ataaagcatt cccgcgcatt tttgcgcgcc agaacgcaca agctgcgcca tccgcttcac
    22501 tgtggctgac caatatctgc gtgcaaattt gtctggtact catctggctg accggttccg
    22561 attacaacac attgctgacc atcgcctcag aaatgattct ggtgccctat ttcctggtcg
    22621 gcgcgttctt gctgaaaatc gcaacccgtc cactgcataa agcggtaggt gtcggtgcct
    22681 gcatttatgg cttatggtta ttgtacgcct ctggtccaat gcacttgctg ttatccgttg
    22741 ttctgtatgc accggggctg ctggttttcc tgtacgcgcg taaaacgcat acccatgata
    22801 atgtactgaa tcgtcaggaa atggtgctga ttggcatgct gttaattgcc tcagttccgg
    22861 cgacctggat gctggtggga taacgttcat cccatcgttg gatagcaaag gagataacga
    22921 tgggtaaagc ccaacccttg ccaatattaa ttactggcgg aggtcgtcgc atcggcctcg
    22981 ccctcgcctg gcatttcatt aatcaaaagc aaccggtgat tatcagctat cggacgcact
    23041 atcccgccat tgatggactg attaacgcag gtgcgcagtg tattcaggct gatttttcga
    23101 ccaacgacgg tgtgatggcg tttgccgatg aagtactaaa aagcacccat ggcctgcgtg
    23161 ctattttgca taacgccagc gcgtggatgg cagaaaaacc aggtacacca ctgaccgacg
    23221 ttctggcttg catgatgcag atccacgtta ataccccata cctactcaac catgcactgg
    23281 aaagattact gcgtgggcac ggacacgccg ccagcgatat cattcacttt accgattatg
    23341 tggtggagcg cggtagcgac aaacatattg cctatgctgc aagcaaagcg gcactggata
    23401 atatgacccg ctcgtttgcc cgcaagctgg caccggaagt gaaagtgaat tctattgcgc
    23461 catcgctgat cctgtttaat gagcatgatg atgccgaata ccgacaacag gcgctgaata
    23521 aatcactgat gaaaaccgcg cctggcgaga aagaagtgat cgacctggtc gattacttac
    23581 ttaccagctg ctttgtcacc ggacgcagtt tccctcttga tggcggtcgt catctgcgtt
    23641 aatgcagctt tatccagcat ataatcaaca cccatgcgct tatcccccag catgcaacag
    23701 aaccaacaaa agcggcgggc aagcgcatca ttccggtgaa ataccacagc gacgccaggt
    23761 aaacaaaata gggaataatc gaccacatgc taaaaatgat ggttgcgcgt aaggcttcaa
    23821 tgccgcgttc gctggcaaca atataatgcg cgatgagcgc aaaggtcggg aaaagtggta
    23881 tcagcccggc gatataataa tttttcgttt ttgctaaaac accaatcaac agcactacca
    23941 gcgcaccgag cgcggctttg attaccagcc ccatcttttt accttaacac ttccataaca
    24001 agttatcagt agaatacctg atgaaacctt gtttagaaac gattgatagt aagtaaaaac
    24061 agcgcggtgt attgtgacgt ttttatatct accgtgaatg ttatgaacac tatcgtattt
    24121 gtggaagatg atgcggaagt cggttcactg attgccgcgt acctggcaaa acatgatatg
    24181 caggttaccg tggaaccgcg cggcgaccgg gccgaagaaa ctattctgca agaaaatccg
    24241 gatttggtgt tactcgacat catgctacca ggcaaggacg gcatgaccat ttgtcgtgat
    24301 ttacgcgcaa agtggtccgg accgattgtt cttctaacct ctctcgatag cgatatgaac
    24361 cacatcctgg cactggaaat gggtgcctgc gactatattc tcaaaacgac gccccctgct
    24421 gttttgttag cgcgtttacg tttgcatttg cgtcagaatg agcaagccac actgagcaaa
    24481 ggtcttcagg aaacgtctct gactccctac aaagccctgc atttcggcac gttgaccatc
    24541 gatcctatca accgcgtagt caccctggct aacactgaaa tctcgctctc gacagctgat
    24601 ttcgaattat tgtgggaatt agctacccat gctgggcaaa tcatggaccg cgatgcattg
    24661 ctgaaaaatt tacgcggcgt cagttatgac ggactggatc gtagcgtgga cgtggctatt
    24721 tcgcggttaa gaaaaaaact gctcgataac gccgcagaac cttatcgcat taaaactgtt
    24781 cgtaacaaag gctatctttt tgcgcctcat gcatgggaat aagcgatgaa aaaactgttt
    24841 atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct ggttgggctg
    24901 gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt gatgaacagt
    24961 tcgctgtatc tgatgcgcag cgaattgcgt gagatccccc cacacgattg gggtaagacg
    25021 ctgaaggaga tggatttaaa tctctctttc gatctgcgag tcgagccact gagtaaatac
    25081 catcttgatg atatttccat gcaccggctg cgtggcggcg aaattgtcgc cctggacgat
    25141 cagtacactt ttttgcaacg tatcccgcgc agccactacg tgctggcagt tggtcctgtt
    25201 ccttatcttt attacctcca tcagatgcga ttactggata tcgccctgat cgcttttatt
    25261 gccatttccc tcgcctttcc ggtgtttatt tggatgcgtc cgcactggca ggatatgtta
    25321 aaactggaag cagcggcgca acgatttggc gatgggcatc tcaatgaacg tatccacttt
    25381 gatgaaggtt cgagctttga acgacttggc gtcgcattta accagatggc ggacaatatc
    25441 aacgccttga ttgccagcaa aaaacagctt attgacggta tcgctcacga actgcgaaca
    25501 ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc cgtcgaatcc
    25561 caggcgttga atcgcgatat cagtcaactt gaagctttaa ttgaagagct gctgacttat
    25621 gcccgactcg atcgcccaca aaatgagctt catcttagcg aaccagacct gccgttgtgg
    25681 ctgtcaacgc atctggcaga tattcaggca gtaacgcccg ataaaacggt acggataaaa
    25741 acgctcgcgc aaggccatta tgctgcgttg gatatgcgct taatggagcg cgtgctggat
    25801 aatttgctca ataacgccct gcgctactgt cactcaacgg ttgaaaccag cctgctactg
    25861 tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc ccctgaaaac
    25921 cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg ctcaaccggc
    25981 ggctgcgggc tggggctggc aattgtccac tctatagcac tggcaatggg cggtacggtt
    26041 aattgtgaca ccagcgaact gggtggtgcc cgcttctcgt ttagctggcc gttatggcat
    26101 aacatcccgc aatttacctc tgcttgacat tacgcgcacg gtggtcaagt caccaccact
    26161 gtgctataaa ataaggatgt tgtaactaaa gtggttaata ttatggcgcg ttacgatctc
    26221 gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctgc atttgccgct
    26281 gagctagaac agcacaaatt gctggttgcc cgcgtgttct ctttgccgga ggtaaaaaaa
    26341 gaggatgagc ataatccgct taatcgtatt gaggtaaagc aacatctcgg caacgacgcg
    26401 cagtcgctgg cgttacgtca tttccgccat ttatttattc aacaacagtc cgaaaatcgc
    26461 agcagcaaga ccgctgttcg tctacctggc gtgttgtgtt accaggtcga taacctttcg
    26521 caagcagcgt tggtcagtca tattcagcac atcaatcaac tcaagactac gttcgagcat
    26581 atcgtcacgg ttgagtcaga actccccaca gcggcgcgtt ttgaatgggt gcatcgtcat
    26641 ttgccggggc tgatcaccct taatgcttac cgaacgctca ctgttctgca cgatcccgcc
    26701 actttacgtt ttggctgggc caataaacat atcattaaga atttgcatcg tgatgaagtc
    26761 ctggcacagc tggaaaaaag cctgaaatca ccgcgcagtg tcgcaccgtg gacgcgcgag
    26821 gagtggcaaa gaaaactgga gagagagtat caggatattg ctaccctgcc acaggacgcg
    26881 aagttaaaaa tcaaacgtcc ggtgaaggtg cagccgattg cccgcgtctg gtacaaggga
    26941 gatcaaaaac aagtacaaca cgcttgccct acaccgctta ttgcgctgat taatcgggat
    27001 aatggcgcgg gcgtgccgga cgttggggag ttgttaaatt acgatgccga caatgtgcaa
    27061 caccgttata aaccacaggc gcagccgctt cgtttgatca ttccacggct gcacctgtat
    27121 gttgcagatt aacccccggc tttcatactg ccgaccatct gttctggccg tacccagctg
    27181 tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa ggtcagccct
    27241 tctttatgcg cttttttggc gatctcggcg gctttgtcat aaccaatgtg ggtgttaagc
    27301 gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg attcggttca
    27361 atgcccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa gcgcaccgat
    27421 tgcaggaaat tatggatcac catcggacgg aagacgttca gttcaaagtt accggaagcg
    27481 ccacccatgt tgatcgccac atcgttcccc atcacctgac agcagagcat ggttaatgct
    27541 tcacactgcg tcgggttcac tttccccggc atgattgagc tacccggctc attttccggg
    27601 attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac atcattggca
    27661 attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac cagggcatca
    27721 caggtcgcca gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca agtaatgact
    27781 gccagttcat ctgccacgcg acgcgcatac tccggatggg tatttagtcc agtacccacc
    27841 gctgtaccgc caagagccag ttccgctaca tgcggcaggc tgtattcgat atgtttcaga
    27901 ttatgctcga gcatcgctac ccagccggaa atctcctgcc ccagcgttaa cggcgtggcg
    27961 tcctgcaagt gggtacgacc gattttgacg atatcagcaa atacgcggga tttttcactc
    28021 agcgtctggg tcagggtttt aagttgtgga atgagttgct tgcgcagcgc cagtagtgcc
    28081 gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt cacgtcgtcg
    28141 ttagggtgaa ctttacgttc catcccgcgc acgccaccga gtaattcact ggcccggtta
    28201 gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg ccagatagcc
    28261 agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg ccgaatggcg
    28321 ctcgctttct cttcagacaa caagcctaaa tcttcattaa cttttgccgc tgcgcgcttg
    28381 gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat gcggaaatgc
    28441 tccagcgagc gctgagtttg tgcgccccac agcttatctg ccgggacatc aatcgctccc
    28501 atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctga ttaatttttt
    28561 ctttctgttt tgctttcgtt aagcaacttt tagcttactc attatttacc atttgataac
    28621 aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct ctgtttattt
    28681 cacacagcgg gtgcactgtg tgagttgtat ctgctggaag aagtcatttc ctttatcatc
    28741 cacaaggata aatgccggga aatcttccac ttcaattttc cagatggctt ccattcccag
    28801 ttccggatat tcaacacatt ccaggctctt aatgcttccc tgcgccaata cagctgccgg
    28861 accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg tcacctgctg
    28921 gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca gttgatcgac
    28981 ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat aaccttccgg
    29041 cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atatactgcg gcagcccttc
    29101 gccgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccaa cgataatcgt
    29161 gccattcagc gataagcgtg tagaaacggg atactgcgac aactgtgcga ggatctcttt
    29221 catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac gcagctcttc
    29281 cgggatatat ttgcctggat tatgttccag tttttcgatc cagatcccct gacggttgat
    29341 cttcgctttg atattacggt cagcagagca ggagacgccc ataccgaccg ggcaggatgc
    29401 gccgtgacgt ggcaggcgaa tcacgcggat gtcgtgagcg aagtatttac caccaaactg
    29461 cgcacccaga ccaagatttt gcgcttcgat cagcaattct ttttccagtt ccacatcgcg
    29521 gaacgcctga ccgtgctcat tcccttccgt tggcagttcg tcatagtatt tagcggaagc
    29581 cagtttcacc gttttaaggt tcgtttccgc agaagttcca ccaattacga acgcaatatg
    29641 atacggagga caggccgccg tacccagcgt gcgcatcttc tcaaccaggt aatttttcag
    29701 tttccccggc gttagtaacg ctttggtttc ctgatagaga tacgtcttgt ttgccgaacc
    29761 accacctttg gcgatacaga ggaatttgta ctcgtcgcca tcaacggcat aaagatcgat
    29821 ctgcgctggt agattggtgc cggtattcac ctctttatac atatccagcg gcgcgttttg
    29881 cgagtagcgc agattatctt cgatataagt gttatagaca ccgcgtgcca gcgccgcttc
    29941 atcaccgcca ccggtccata cacgctgccc ttttttgcca acaataatcg cggtgccggt
    30001 atcctgacag gttggcagaa cgcctttcgc cgcgatgtcg gagttgcgca ggaattgcag
    30061 cgccacatat ttatcatttt cgctggcttc cgggtcacgc agaatgtcgg ccacctgttg
    30121 ttggtgagcc ggacgcagca taaatgacgc atcatgaaac gcctgacgcg ccaacaaagt
    30181 taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata cgctaacgtg
    30241 ttcgctggtt agcaggtaat actcagtatc atcttttttg agtggaaaag gagcctgata
    30301 atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt atgctctggg
    30361 cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacactt ttttaacaaa
    30421 aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat tcttgcttta
    30481 atagcgggat taatttccac attaaaacag ggattgatca tgcaaaaact cattaactca
    30541 gtgcaaaact atgcctgggg cagcaaaacg gcgttgactg aactttatgg tatggaaaat
    30601 ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccaaaaag cagttcacga
    30661 gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattgagag taataaatcg
    30721 actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct gttcaaagta
    30781 ttatgcgcag cacagccgct ctccattcag gttcatccaa acaaacgcaa ttctgaaatc
    30841 ggttttgcca aagaaaatgc cgcaggtatt ccgatggatg ccgccgagcg taactataaa
    30901 gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc gatgaacgcg
    30961 tttcgtgaat tttccgagat tgtctccctg ctccagccag tcgcaggtgc acatccggcg
    31021 attgctcact ttttacaaca gcctgatgcc gaacgtttaa gcgaactgtt cgccagcctg
    31081 ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc ggccctcgac
    31141 agccagcagg gtgaaccatg gcaaacgatt cgtttaattt ctgaatgtta cccggaagac
    31201 agcggcctgt tctccccgct gttgctgaat gtggtgaaat tgaatcctgg cgaagcgatg
    31261 ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga agtgatggcg
    31321 aactccgata acgtgctgcg tgcaggtctg acgcctaaat acattgatat tccggaactg
    31381 gttgccaacg tgaagttcga agccaaaccg gctaaccagt tgttgaccca gccggtaaaa
    31441 caaggtgcag aactggactt cccgattcct gtggatgatt ttgccttctc gttgcatgac
    31501 cttagtgata aagaaaccac catcagccag cagagtgccg ccattttgtt ctgcgtcgaa
    31561 ggcgatgcaa cgctgtgtaa aggttctcag cagttacagc ttaaaccggg tgaatcagcg
    31621 tttattgccg ccaacgaatc accggtgact gtcagaggcc acggccgttt agcgcgtgtt
    31681 tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc ttattaaagg
    31741 cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa tcattatgaa
    31801 taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga caggcggcgc
    31861 atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc aggcgaacgc
    31921 gcaactcaaa ctgaccgctc ctgaatccaa cctggaagtg agttatcaaa actatcatcg
    31981 cggcgtattc agcagtcagc tgcaactgtt ggtgaaaccc attgccggaa aagtaaatcc
    32041 gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg gtcccttccc
    32101 gcttgcccag cttaaaaaac tgaatctgat cccgtcgatg gcatcaattc aaaccacgct
    32161 ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa cgccttttga
    32221 gattaactcc cgcattggtt acagcggaga ttccagttcc gacatttcgc tcaagccact
    32281 gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc agttaaatgc
    32341 agacagagac ggcaaagcta tctccctttc cggggaggcg caaagtggtc ggatagacgc
    32401 tgttaacgaa tacaaccaga aagtacagtt gacctttaat aatctgaaaa ccgacggttc
    32461 cagcacgctg gcaagttttg gtgagcgcgt aggaaaccaa aaactgtcac tggaaaaaat
    32521 gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga tcagcggtaa
    32581 atcggatctg gtcaatgatg gtaaaacgat caatagccaa ctggattact cgctaaacag
    32641 cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag tcggccaaat
    32701 tgatggcgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc aggcgctgct
    32761 ggcacagcca gaaattgcca acaatcccga actttatcag gaaaaagtga cggaagcctt
    32821 ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactatcg cgccgctaag
    32881 ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga aagatccggc
    32941 aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gaccgttcgg ttaaatctct
    33001 ggatgcgaaa ctgaccattc cggtggatat ggcaactgag ttgatgactc aggtagcgaa
    33061 gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag ttgaaggtgc
    33121 atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca tcaccaccag
    33181 cctgcaatat gctaacggtc agatcacgtt aaacgggcag aaaatgccac tggaagattt
    33241 tgttgggatg tttgcgatgc cggcattaaa cgttccggct gtacccgcta ttccgcagca
    33301 gtaattcaca gccaaaatgc cggagatttt tctctccggc attttttctt acttcagcat
    33361 aaagtcatac tgaatactca ttttcacatc ccagtaattc aggcgatcgc ccagcgcact
    33421 gccatcgcgc ttttcccgcc ctttatcggc agcaagaaag aaattcatcc cttgtaacgg
    33481 cccttcgctg acccgatagt tcataccacc taagataccc gcgccttcaa tccgcacatc
    33541 tcgtttactg gattcatagc cgcgagtcac aatcggtgca aatgtcagcg taaattgcgg
    33601 cgttaaacga tagttgacac ccaattgcca ggactgcatt ccttcacggt tacgatcaag
    33661 acttaatgaa aagggatagc caatgtcggt atccaccacg gcaccattca ttgcatggcg
    33721 caaataacca ccactgccaa atacgcttcc ccacggcgca ttccaggtca attgtccact
    33781 aaccaacgcc gtttcattgg gctggctggt attacgactt aaaccttcaa gttgcgcgta
    33841 aaagccgagc aactcccctt tcaacgtata attcgggtca agcggtatgc ttttctcgag
    33901 aatactttgt atctgattgg aaacatcttt ctgtaccgat gcccccagag aatagcgact
    33961 ggtatcatca tgccaactgg cggctaaaaa agaacgtgcg cgatgtttat cgtattttcc
    34021 cgtctggctg ttatacaccc cttcatcacg aataccggtt gttgaatcgg tccataaaaa
    34081 tactcgtgac cgccacattc cccacagatc aatatttggc gttaaagccc agtaacctaa
    34141 tgctccttca gaaactgtcg gtaatgtacg ttgcgaactg ccagccatta acggaatacg
    34201 ataaggtaag ctaccataac tataatccgt cccgaagcga cctaatttta gcaggctatt
    34261 ttcactgggt tttattttta ctgcccccag ggcaaagcca aaacttttat caccatcaag
    34321 ataccaccgg gtactcatat cagcacgagc acctaattta taaacataat acgccccgcc
    34381 ttcaacgcca ataatattgg aataataacc actattgaaa tcgagcaatc ctccctgtac
    34441 ccatgcgtaa atatctcttc ccgtgccagc actgggctta tcagccctgc gtaattcgtt
    34501 tttcagtctg atgcgtagtc ccgcttcatc gtcagccggt tgcgccgcgt aagcagaggt
    34561 aaggccagag gcagccgtca ggcaaataac ggccatggcc actatttttc tcatagttgc
    34621 acctttgatg ttctgattat ttttattgaa tattaactag tgatatcgtt gattaattgc
    34681 tgctgaactt ttttacgatt atcaatttca acaacgattt ctttgaattt tttatccgtg
    34741 agcggataaa accagataat gacgaatgcc agtagcataa atccgcaagg tactaaggca
    34801 attgatgtgc ggatgcccat aataacttcc ggcgtttgcg cctgattggc gatatatccg
    34861 cttaatccca aaataaaggc aggaattgaa cctccgattg cctgaccgca tttacgggta
    34921 aatgagaata gtgaataggt gagcccttca attcgcacgc cggtcaggta ttcaccgtat
    34981 tctacggtat cagcttccag tgcccacatc acggtcatgg taacgccctg gccaattgat
    35041 gcgatggcca acgcaaccaa cgccaccggc agtgaccaga cggaaaccca gaagaacagc
    35101 agataaccgc aggttcccag caaagcgcca atcaggaagg tattcttttt accgatcctc
    35161 gcgaccatcc ccggcaccag cggtgccgat gccacagtac cgaccaggtt ttgcaccagt
    35221 accagcacag tgaacagccc ggtatcattt aacacatagc gcacgtagaa caacgacgag
    35281 gcgctgaccg cgaaggtcga aatcagcaca cacagcgcac cgatgcacaa cataaacagc
    35341 gggcgattcc gtttcagggt ttgcagactg atcttcaatg acggctgcgc cacgatacgt
    35401 accacattct cacgcgtcga tttgaagcag atgaagtaaa gcaccattcc ggcaatcgcc
    35461 agcacaattg tccagaaatg atataccgac accatctctt ccgggctgga gttcttaatg
    35521 ctcggtccta tcagaaatgc caggcagaca aaagtcaatg aagcggcaat cccgcgagcc
    35581 gcgcccagac gggcgcggga ttgtggttgt tgggtcattg cggtagcaag tgaaccataa
    35641 ggaatattca ccaggctgta gcaaagcccg aggcccatgt aagtcaaata tgcatacact
    35701 actttgctgc tatggctcca gtcggtcggt acccagaata ccagcacgct gaagatcatt
    35761 aacggcgcag taccgaagag taaaaacggg cggaattttc cccatcgggt attcacactg
    35821 tccaccactc gtccggcaaa gacatcggcg aaggcatcga atacccgcac cagtaacagc
    35881 atggtgcccg ccgcagcggc accgacgcca gcgacgtcgg tgtagtaact caacaggaag
    35941 agcgccccca ttgcgaaggc gaagttattg gcgacgtcac cgaggctgta gccgacgatg
    36001 gcgcgccagg agagttgttg attcattgtt tgcctccctg ctgcggtttt tcaccgaagt
    36061 tcatgccggt ccagcgtttt tgcagcagaa aagccgcaga cttcggtttg cggtcgcgag
    36121 tgaagatccc tttcttgtta ccgccaacgc gcaatatgcc ttgcgaggtc gcaaaatcgg
    36181 cgaaattcca tacctgctca ccgacgacag cgctgacgcg atcaaagacg cggtgataca
    36241 tatccagcca tgcacactga tactcttcac tccacatgtc ggtgtacatt gagtgcaggc
    36301 cggctaacgt atccacaccg tattcggtga tgataatcgg ctgatgcagt ttctcctgcc
    36361 aggccagaag ttctttttcc agaaccttct ctgccgtttc caaatcgccg ctttggacat
    36421 accaaccgta ataacggttc aggcacagca catcaaagag atcgctgatg gtatcggtgt
    36481 gagcgtcgca gaacattaca ttgacgcagg tgatcggacg cgtcggatcg agtttacgcg
    36541 ttgcttccgc cagtggcgcg aaatattccc gtgcaccttg cggacgggta tccggttcgt
    36601 tggcaatact ccacatcacc acgcttgggt ggtttttgtc acgcgcaatc agctctttaa
    36661 tcgcctgtaa gtgcgcctgc tgggtttccc cgttgactgc ctcttcgctg tacagttctt
    36721 tcggcttgtt gcccgcttcg aaaccaatgc ctaaagagag gttaaagccg acagctgcag
    36781 tttcatcaat caccacgatg ccatgttcat ctgcccagtc gagcatctct tcagcgtaag
    36841 ggtaatgcga ggtacggtag gagttggccc caatccagtc cattagtgcg tgatcgtgca
    36901 ccatcagcac gttatcgaat cctttgccgc gcaaatccgc atcttcatga cgaccaaagc
    36961 cagtaaagta gaacggtttg tggttaatca ggaactgttc gtccttcact gccactgacc
    37021 ggatgccgac gcgcagcggg tagatatcac actctgtctg gcttttggct gtgacgcaca
    37081 gttcatagag ataaccttca cccggttgcc agaggtgcgg attcaccact tgcaaagtcc
    37141 cgctggtgcc ttgtccagtt gcaaccacct gctgatccgc atcacgcagt tcaacgctga
    37201 catcaccatt tgctaccacc tgccagtcaa cagacgcgtg gttacagtct tgcgcgacat
    37261 gcgtcaccac ggtgatatcg tccacccagg tgttcggcgt ggtgtagagc attacgctgc
    37321 gatggatccc ggcatagtta aagaaatcat ggaagtaaga ctgctttttc ttgccgtttt
    37381 catcggtaat caccattccc ggcgggatag tctgccagtt cagttcattg ttcacacaaa
    37441 cggtgatacg tacacttttc ccggcaataa catacggcgt gacatcggct tcaaacggcg
    37501 tatagccacc ctgatgctcc atcacttcct gattatttac ccacactttg ccgtaatgag
    37561 tgaccgcatc gaaacgcagt acgatacgct ggcccgccca acctttcggt ataaagactt
    37621 cgcgctgata ccagacgttg cccacataat tacgaatatc tgcatcggcg aactgatcgt
    37681 taaaacttcc cggcacagca attgcccgga tttcttgtaa cacgctttcc caccaacgct
    37741 gatcaattcc acagttttcg cgatccagac tgaatgccca cagaccgtcg agttttttga
    37801 tttcacgggt tggggtttct acaggacgta acataaggga ctcctcatta agataaaaat
    37861 actggtcaac ctttaatctg attagttaac caattctgtg ttgcacacag ttatctgtga
    37921 gaaacccatg gaagagaaat ggaaataaaa agaactgaca ccgggatcaa aaaaccaaaa
    37981 actgcaatac ccacagcgtg atatagatcg cattaatctt taaaacggcg ttgacagaag
    38041 ccataaaata agcaaccgaa cgttcgttcg agcataatgg gaacgataaa ctctcatgac
    38101 atatggctac agtgaatatt ttggggggaa ttctccgaac ccgggaagga taatttttgc
    38161 acagagaaat tacgatatga aacgcgggca cagttatcac cagcccgcga aatgagagga
    38221 tcaggatgcg gttaagatac cgccaatcat cgcccgcaat ccctgagcaa acgacgcttc
    38281 acgcgacaga tcttccgctt cgatatcggc cagtacaccg taggtcagat ccagcagtaa
    38341 acgtgaagtc attgccgtgt tgatgtctgg gcttatctcg cctttttgct gggcatcacg
    38401 catccgctgg gcaacaaatt ccgtgatcgt catatgctta tttttaagca tggcggcgac
    38461 ctgtgggtta cgcatccctt ccgccataat ttcgaccacc agcgcccgtt gtccaaaggc
    38521 ctcatgggtg agagagacaa tagactcgac catatagtca acgaaatgaa tcccttcaat
    38581 cggttcccgg aaacgggcca gcgccctctc ctggtcctgt aagataatcg cctgaatcaa
    38641 ggcttctttg gagatgaaat ggtgatagag cgtcccggga ctaatggcgc aagatttaca
    38701 gatggctttc atcgaggcac tgtgaaatcc attttctgaa aaaatctctc tggcagcatt
    38761 gaggatccgg gtccgtgtcg gttgtgcttc agtctgcatg ttatccatca ttaaaccatt
    38821 atagttagcg tgaataacta gtttaccgcg ttgtcaggtg agcgcctatc attgctgttg
    38881 aatagtgcga aggcacactc tattcgtata cttttcactc ggaaattggt taaccaatag
    38941 ccgtaatttg gctgccagtt atttagctga gacgttgatg agtttatccc ggtaaacaac
    39001 acgcacggtt gtttaccggt ccgttagtgt attaattgag ctcctgtacc ccaccaccgg
    39061 agacggtgag aatttgtccg cttacccagc tggctgcagg cgagcaaagg aacagcgccg
    39121 cgttagcaat atcttgcggt tggcccagac gtctgatcgg cgtgtgctgt aacatttttt
    39181 gttcaatttc tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa
    39241 tgccattcac ccgaatattt ttttcaccca ggtcaaacgc catatttctg accagatgac
    39301 tggccgcagc tttagatgat gcataagaag tcatgtttat atttttattt tctgccgcca
    39361 tagaagtgat ggtcagaata acgccaccgc catttttttc catttctggc gcaacaagtt
    39421 gtgacagatg gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg
    39481 gcatatcaaa cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac
    39541 ccagcttact gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac
    39601 aacggcaggc aaatgcctga ccacccagtt gttgaatttc atcaacaaca tggttagctg
    39661 cgtcggcgtt aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga
    39721 tttctttacc aatacctgca ccggcacctg tgatgatggc acattttccg tcgagtctca
    39781 ggttgtcaga attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc
    39841 ctgtgtgcca cattttttat cattaacctt gaggaaagga cactaccagg tatagcagat
    39901 aaagagagga ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg
    39961 tctcttcatg ggtgattttt tgcatcatgc gatccgcaag tgtggtacca agttcccgcg
    40021 caggcgtgct ggcccaggta acaggtatat catcaagtgt ggttggcgtt gcatcggtaa
    40081 atgccgccaa cgaaacctgt tgttcaaagt aacgatcgac tccgctttcc ccgctttgcc
    40141 gccctgcttt cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca
    40201 cagcactgat ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct
    40261 ggctggaaga gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag
    40321 ttgcacaata gccccccacc cgttctgccc gggttaatga ggaactttgc cctcccagcc
    40381 aggcgatacg ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca
    40441 tgttatccgg gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca
    40501 ccgggatagc tttttcttct gccatccgtc gcaggtcatc gctgcttcct gcagccccgg
    40561 caatgaccac accatcgacc ccctgattca gtaacagtga aaaccgctgt gccagctgct
    40621 cgccgtcttt accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg
    40681 tcaatccggc cgttaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa
    40741 tgacgccgct ttgcccgccg cgcagcgccg acgcctgacg attgcgcaca aatcccagct
    40801 cttcaatggc ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactta
    40861 gcaccagcga aacggtgctt accgacacgc ccgcagccag cgcaacatca tgaatggtta
    40921 tttttttggc ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt
    40981 ttacctttta tttatcttat acccgctatt atcgttgcgt aatgtgattt atgcctcact
    41041 aaaatttgat aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac
    41101 gaggagtcgt tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca
    41161 gcagttaggc aaaaccttca tgctacctgt ggcattattg tcgttctgcg gcattatgct
    41221 cggcattggt agttctctta gcagccatga tgtcatcacc ctgatcccgg tcctgggcaa
    41281 ccccgtgttg caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt
    41341 cctgccagtc atgttctgta tcgccatccc gctgggcctg gcacgcgaaa acaaaggtgt
    41401 agcggcattt gctggctttg tcggttatgc ggtaatgaat ctcgcggtaa acttctggtt
    41461 gaccaataaa ggcattctgc caaccacgga cgccgcggtt ctgaaagcca ataacattca
    41521 gagcattctt gggatccagt cgatcgatac agggatcctc ggtgcggtga tcgccggtat
    41581 tatcgtctgg atgctccatg agcgtttcca taatatccgc ctgccggatg cgctggcatt
    41641 cttcggcggt acgcgcttcg tgccaatcat ctcctcgctg gtgatgggcc ttgtcggtct
    41701 ggtgattcca ttagtctggc caattttcgc catgggtatt agcggcttag gccatatgat
    41761 caacagcgcg ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc
    41821 gtttggtctg catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca
    41881 ggaagtctgc ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg
    41941 cccgaccact cacggttttt ctgaaagcgc cacgcgtttc ctttcgcaag gtaaaatgcc
    42001 tgcgtttctc ggcggcctgc caggtgcagc gttagctatg tatcactgcg cgcgcccgga
    42061 aaatcgccat aaaattaaag gtctgcttat ttctggcctg atcgcctgtg tcgttggcgg
    42121 cacgaccgaa ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca
    42181 cgcgctgtta accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa
    42241 taccgacggc aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa
    42301 gtggtacatg gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg
    42361 tttcgctatc acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc
    42421 aatcgaaaaa gccgttgccg gtgcgccggg taaatcaggt tacaacgttc cggcaatcct
    42481 cgaagcctta ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg
    42541 tttgtctgtg aaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat
    42601 tggcgtagta caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc
    42661 agtaaaagat gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt
    42721 cgatttttca aaggtcgtgg atcgtcatgg cacatggtgt acgcagtggg attatgtcgc
    42781 tgaccgtttc ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgcctc
    42841 tgccccctgc attatcgagg cgctgaatca gcgcctgatg cacggcgtgt ttggctacag
    42901 ccgctggaaa aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta
    42961 caccgcaatt gatacgcagt cggtggtgta tggcccttct gttatctaca tggtctcgga
    43021 actgattcgt cagtggtctg aaaaaggtga aggcgtggtg atccacacac ccgcctatga
    43081 cgcattttac aaggccattg aaggtaacca gcgcacagta atgcccgtcg ctttagagaa
    43141 gcgggctgac ggctggtttt gcgatatggg caagctggaa gccgtgttgg cgaaaccaga
    43201 atgtaaaatt atgctcctgt gtagcccaca gaatcctacc gggaaagtgt ggacgtgcga
    43261 tgaactggag atcatggctg acctgtgcga gcgtcatggt gtgcgggtta tttccgatga
    43321 aatccatatg gatatggttt ggggcgagca gccgcatatt ccctggagta atgtggcgcg
    43381 cggagactgg gcattgctaa cgtcgggatc gaaaagtttc aatattcccg ccctgaccgg
    43441 tgcttacggg attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg
    43501 acgtgatggg ctttcttccc cttcggtact ggcgttaact gctcatatcg ccgcctatca
    43561 gcaaggcgca ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat
    43621 cgcagataaa atgaacgccg cgttccctga actcaactgg cagatcccgc aatccactta
    43681 tctggcctgg cttgatctac gtccattgaa tattgacgac aacgcgttgc aaaaagcgct
    43741 tatcgagcaa gagaaggtcg cgatcatgcc ggggtatacc tacggtgaag aaggtcgtgg
    43801 ttttgtccgt ctcaatgccg gctgcccgcg ttcgaaactg gaaaaaggtg ttgctggatt
    43861 aattaacgcc atccgcgctg ttcgttaacc ccaattgcgc aacgtaaaaa atcgttgcgc
    43921 aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa
    43981 agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc caccttgatg
    44041 gcaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg
    44101 cacaatccct ggaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg
    44161 tgagctttct gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcttgcc
    44221 gccgcgtggc atttgaaaac attgaagatg cagcccgtaa tggcctgcac tatgtcgagc
    44281 tgcgtttttc accgggctac atggcaatgg cacatcagct gcctgtagcg ggtgttgtcg
    44341 aagcggtgat cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta
    44401 tcggcattat gagccggacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt
    44461 tagcccaccg tgaccagatt accgcacttg atttagccgg tgatgaactt ggtttcccgg
    44521 gaagtctgtt cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc
    44581 atgcaggcga agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggggcgg
    44641 aacgtattgg acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg
    44701 agcagcaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtggcag
    44761 agctggcggc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca
    44821 ctgacgatcc cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccgg
    44881 ctgctgggtt atcccgcgag caaatccgcc aggcgcagat taatggtctg gaaatggctt
    44941 tcctcagcgc agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat
    45001 ggatggtgct aattgcacca tcctcttttc acgcaaggca cagcgtcgcg cgatgtttgg
    45061 cggattcgat gcccagctca atcaactcca ttacctggat tgcctgactt gccggaaccg
    45121 gattttcacc atcgccattt aacgcatcac gaatagccgc atagtaagcc ggataattcc
    45181 ctggtactgt caacagcgtt tcttcgatac gttcctcacc ttccacgcgg gtaagtacgc
    45241 catcacgcat atcgtagccc cagccttcct gcggcagacg ctcgccattt ttcagtcgtt
    45301 cttcctgtgg atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatataac
    45361 gtgctgactc agcagctgcc agcatggtac cgtgtaaaat gactcgccgc tgcggatagg
    45421 acaagatggc gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta
    45481 ccgtcatgct gaccggtaaa ccaaatagcg taatggcctg atcaagaagg tgcggtgcta
    45541 aatcgtacca gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgaacctgcg
    45601 gacggaagcg gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga
    45661 gcaaaccttt tagcgtcagg aaatcgctat cccagcgacg gttatggaat acagacagca
    45721 cacgccctag gctttttgcc agcgcttcca gctctcgcgc ttgtgacagt gtcacggtaa
    45781 agggtttatc aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat
    45841 gggtatcgtt gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct
    45901 tcggctcaga gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcgctgc
    45961 tggagattac cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt
    46021 tgctcgcata accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc
    46081 tcattgaagt atgatggcta tttgacacta tcctttaccc tcgctcaaca gtttaataac
    46141 ctgccagcaa taagggatgt tgtttaactt aagtcaaaaa aatagcgaat tttccaacga
    46201 caaaagctaa atatcgcaaa aacctcagta aaaatcttgc tggagctatt attgctaagt
    46261 aacatttacc ccctgaagtt aatggatcaa tcaacagaga tgtgggctgt aatgaatcgt
    46321 cttattgaat taacaggttg gatcgttctt gtcgtttcag tcattcttct tggcgtggcg
    46381 agtcacattg acaactatca gccacctgaa cagagtacta cggtacaaca caagtaagct
    46441 ctgcgcttgt agagcgacat gctgcccgtc cgggtgcatg ttttcacttg tcggatatta
    46501 aaccaggaat ttattatctt gttcgatgtt gttggtgatt gtcagagata gtaaagcgcg
    46561 ttactctttt cagagcagtt tttcatccct gaatatttgc catcagcgca t
//
LOCUS       DAZKGF010000015        45943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZKGF010000015 DAZKGF010000000
VERSION     DAZKGF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.015"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(30..290)
                     /gene="yfhL"
                     /locus_tag="ACHOQV_000845"
     CDS             complement(30..290)
                     /gene="yfhL"
                     /locus_tag="ACHOQV_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFP9629259.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(346..1194)
                     /locus_tag="ACHOQV_000846"
     CDS             complement(346..1194)
                     /locus_tag="ACHOQV_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9629260.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISAYHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            1403..2038
                     /gene="yfhb"
                     /locus_tag="ACHOQV_000847"
     CDS             1403..2038
                     /gene="yfhb"
                     /locus_tag="ACHOQV_000847"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFP9629261.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2096..2599
                     /gene="tadA"
                     /locus_tag="ACHOQV_000848"
     CDS             2096..2599
                     /gene="tadA"
                     /locus_tag="ACHOQV_000848"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP9629262.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2596..4152)
                     /gene="mltF"
                     /locus_tag="ACHOQV_000849"
     CDS             complement(2596..4152)
                     /gene="mltF"
                     /locus_tag="ACHOQV_000849"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFP9629263.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            4410..8297
                     /gene="purL"
                     /locus_tag="ACHOQV_000850"
                     /gene_synonym="purI"
     CDS             4410..8297
                     /gene="purL"
                     /locus_tag="ACHOQV_000850"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFP9629264.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYVEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGASGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            8854..10281
                     /gene="qseE"
                     /locus_tag="ACHOQV_000851"
     CDS             8854..10281
                     /gene="qseE"
                     /locus_tag="ACHOQV_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFP9629265.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIDLPSSKNTK"
     gene            10446..11159
                     /gene="qseG"
                     /locus_tag="ACHOQV_000852"
     CDS             10446..11159
                     /gene="qseG"
                     /locus_tag="ACHOQV_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFP9629266.1"
                     /translation="MRHIFQRLLPRRLWMAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            11149..12483
                     /gene="glrR"
                     /locus_tag="ACHOQV_000853"
     CDS             11149..12483
                     /gene="glrR"
                     /locus_tag="ACHOQV_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFP9629267.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            12544..12882
                     /gene="glnB"
                     /locus_tag="ACHOQV_000854"
     CDS             12544..12882
                     /gene="glnB"
                     /locus_tag="ACHOQV_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFP9629268.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(12927..14117)
                     /gene="hmpA"
                     /locus_tag="ACHOQV_000855"
     CDS             complement(12927..14117)
                     /gene="hmpA"
                     /locus_tag="ACHOQV_000855"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFP9629269.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGLSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            14446..15699
                     /gene="glyA"
                     /locus_tag="ACHOQV_000856"
     CDS             14446..15699
                     /gene="glyA"
                     /locus_tag="ACHOQV_000856"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP9629270.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDASGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(15762..16955)
                     /locus_tag="ACHOQV_000857"
     CDS             complement(15762..16955)
                     /locus_tag="ACHOQV_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9629271.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGCLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATKIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            17073..20354
                     /locus_tag="ACHOQV_000858"
     CDS             17073..20354
                     /locus_tag="ACHOQV_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFP9629272.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLKGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNITLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVTKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKCSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKITSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            20451..21434
                     /locus_tag="ACHOQV_000859"
     CDS             20451..21434
                     /locus_tag="ACHOQV_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9629273.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            21457..22968
                     /locus_tag="ACHOQV_000860"
     CDS             21457..22968
                     /locus_tag="ACHOQV_000860"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9629274.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVSPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKDAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            22993..23991
                     /locus_tag="ACHOQV_000861"
     CDS             22993..23991
                     /locus_tag="ACHOQV_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9629275.1"
                     /translation="MSASSLLLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            24024..25118
                     /locus_tag="ACHOQV_000862"
     CDS             24024..25118
                     /locus_tag="ACHOQV_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9629276.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            25130..26002
                     /locus_tag="ACHOQV_000863"
     CDS             25130..26002
                     /locus_tag="ACHOQV_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP9629277.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKNGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCVSRNDDILC
                     LVYEHRSGVYHYRVSQTFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(26151..26573)
                     /locus_tag="ACHOQV_000864"
     CDS             complement(26151..26573)
                     /locus_tag="ACHOQV_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP9629278.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(26670..27872)
                     /gene="hcaD"
                     /locus_tag="ACHOQV_000865"
     CDS             complement(26670..27872)
                     /gene="hcaD"
                     /locus_tag="ACHOQV_000865"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFP9629279.1"
                     /translation="MKEKTIIIVGGGQAAAMAAALLRQQGFSGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTQELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAASVMGRNAPPPVQRYLLPRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLRADVVIYGIGISANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRIDNGALHRCESWENANNQAQIAAAAMLGLPLPRLPPPWFWSDQYSDNLQFIGDM
                     HGDDWICRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRLIRKWIQSGKTFDAKLLID
                     EDIALKSL"
     gene            complement(27882..28694)
                     /gene="hcaB"
                     /locus_tag="ACHOQV_000866"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(27882..28694)
                     /gene="hcaB"
                     /locus_tag="ACHOQV_000866"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFP9629280.1"
                     /translation="MSDLHNESIFITGGGSGLGLALAERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVNQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(28691..29011)
                     /locus_tag="ACHOQV_000867"
     CDS             complement(28691..29011)
                     /locus_tag="ACHOQV_000867"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFP9629281.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(29011..29529)
                     /gene="hcaF"
                     /locus_tag="ACHOQV_000868"
     CDS             complement(29011..29529)
                     /gene="hcaF"
                     /locus_tag="ACHOQV_000868"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFP9629282.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDGNWRLLERDIVLDQ
                     AVISSHNLSVLF"
     gene            complement(29526..30887)
                     /gene="hcaE"
                     /locus_tag="ACHOQV_000869"
     CDS             complement(29526..30887)
                     /gene="hcaE"
                     /locus_tag="ACHOQV_000869"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFP9629283.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKHGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            31023..31913
                     /gene="hcaR"
                     /locus_tag="ACHOQV_000870"
     CDS             31023..31913
                     /gene="hcaR"
                     /locus_tag="ACHOQV_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFP9629284.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPAIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            32073..33212
                     /locus_tag="ACHOQV_000871"
     CDS             32073..33212
                     /locus_tag="ACHOQV_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFP9629285.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSSLTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(33204..34484)
                     /gene="csiE"
                     /locus_tag="ACHOQV_000872"
     CDS             complement(33204..34484)
                     /gene="csiE"
                     /locus_tag="ACHOQV_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFP9629286.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRKSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRSAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(34675..35529)
                     /locus_tag="ACHOQV_000873"
     CDS             complement(34675..35529)
                     /locus_tag="ACHOQV_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9629287.1"
                     /translation="MALPVNKRVLKILFILFVVAFCIYLIPRIAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLHGWFIPSATGPAEKAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(35674..36477)
                     /gene="suhB"
                     /locus_tag="ACHOQV_000874"
     CDS             complement(35674..36477)
                     /gene="suhB"
                     /locus_tag="ACHOQV_000874"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFP9629288.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            36596..37336
                     /gene="trmJ"
                     /locus_tag="ACHOQV_000875"
     CDS             36596..37336
                     /gene="trmJ"
                     /locus_tag="ACHOQV_000875"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFP9629289.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            37606..38094
                     /gene="iscR"
                     /locus_tag="ACHOQV_000876"
     CDS             37606..38094
                     /gene="iscR"
                     /locus_tag="ACHOQV_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFP9629290.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            38206..39420
                     /gene="iscS"
                     /locus_tag="ACHOQV_000877"
     CDS             38206..39420
                     /gene="iscS"
                     /locus_tag="ACHOQV_000877"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP9629291.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            39448..39834
                     /gene="iscU"
                     /locus_tag="ACHOQV_000878"
     CDS             39448..39834
                     /gene="iscU"
                     /locus_tag="ACHOQV_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFP9629292.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            39851..40174
                     /gene="iscA"
                     /locus_tag="ACHOQV_000879"
     CDS             39851..40174
                     /gene="iscA"
                     /locus_tag="ACHOQV_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFP9629293.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            40270..40785
                     /gene="hscB"
                     /locus_tag="ACHOQV_000880"
     CDS             40270..40785
                     /gene="hscB"
                     /locus_tag="ACHOQV_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFP9629294.1"
                     /translation="MDYFTLFGLPACYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            40802..42652
                     /gene="hscA"
                     /locus_tag="ACHOQV_000881"
     CDS             40802..42652
                     /gene="hscA"
                     /locus_tag="ACHOQV_000881"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFP9629295.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSTAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            42654..42989
                     /gene="fdx"
                     /locus_tag="ACHOQV_000882"
     CDS             42654..42989
                     /gene="fdx"
                     /locus_tag="ACHOQV_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFP9629296.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            43001..43201
                     /gene="iscX"
                     /locus_tag="ACHOQV_000883"
     CDS             43001..43201
                     /gene="iscX"
                     /locus_tag="ACHOQV_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFP9629297.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            43260..44543
                     /gene="pepB"
                     /locus_tag="ACHOQV_000884"
     CDS             43260..44543
                     /gene="pepB"
                     /locus_tag="ACHOQV_000884"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFP9629298.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGTDDLGLI
                     QRAARKIDGLGIKHVQLSGEDWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAARLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            44662..45438
                     /gene="sseB"
                     /locus_tag="ACHOQV_000885"
     CDS             44662..45438
                     /gene="sseB"
                     /locus_tag="ACHOQV_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFP9629299.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(45470..45595)
                     /gene="timP"
                     /locus_tag="ACHOQV_000886"
     CDS             complement(45470..45595)
                     /gene="timP"
                     /locus_tag="ACHOQV_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFP9629300.1"
                     /translation="MKIRCFCIVLIVSGALLSEVNNNRSLSGNNLLAVNNLQSSK"
BASE COUNT        10779 a        11631 c        12672 g        10861 t
ORIGIN      
        1 aattaacact ctacatccag ggtagaatat taaattttat ccgcgtgatg cataagcaca
       61 aatttatccc acaactgttc ttccgtctcg acatgttgcg gatccttcac aatagtattg
      121 gggatcgggc acaccttctg gcaggttggt gtctcgtagt gccctacgca ttcggtacac
      181 ttatcgctgt taatctcgta gatatgatcg cccattgaaa tcgcctcatt cgggcattcg
      241 ggttcacaca tatcacaatt gatgcatttt ttagtgatta acagcgccat aaggaaactc
      301 tcaaaacaac acaaaccggg cgggcattat acgcgcattc tttactcaga ccagtttttt
      361 caccagcgct tcactgtgac gaatacgttc tggagccagt tccagatcct gctgaatcag
      421 cgcaatgaac agcaaatccg ttaacattcc ctgcgcgtga taagcagaga ttgaagcact
      481 gtttgtcgct tgttcttcgg caatggtata caggcaatga gaagcacgct gttgcagggc
      541 attgggagta aagccggtaa tcgccagcac ttttccgccc actcgcagca tctcatctgc
      601 cgccaggttt aactcgcgtc gtacaccggt gtaggaaatg gctaataaca gatcgtcagg
      661 ggacgacgcc tgtactgtag cgagtagcgc atgcatatcg cgcactgcgg cagcattgaa
      721 gccaatcttc atcagcttcc aggcaaagtt ttgcgccacc agacccgaag cgccaatacc
      781 ggtcagaatt atccgccgcg cagagcgcaa cattgccacg cattcatgca gtttctcttc
      841 actattaacg tttaacgttg cgtacatagc ggcagtattt tctttaatca gtttttcgcc
      901 gaccagccgt aacggatcat caccgcggat ttggttatga atgggcacgg agggagattc
      961 cggctggctt gccagcgctt cactcaacgc caacttaagt gccggaaaac ctttatagcc
     1021 gagtttttgc gcgaacttca cgacgctgga ctgactgact ccggcttcgt tggccagttg
     1081 ctgagagctt aaatggcgcg ccgtatcagg ttgtagcagc agataatccg ccagtttttt
     1141 atcactttgg gcaagcccct ggtaacgctg acggatacga agtaagccgt tcatatcttt
     1201 cctcgcgatg aaatagtggc gacaacaggc aatatgagac acacgcgagt gaattttata
     1261 ttccattgcc gctaattgcc atgaattaaa aattccagtt agccatgatg cggtcgattg
     1321 ttgccggaaa agtgatgatc tatctgttag gctatgtagt agcgaaaatt aatcaggatg
     1381 tttcagtcca gaggagtatg gtttggcaac tcacgagcgt cgtgtggtgt tttttgactt
     1441 agatggaaca ttgcatcagc aggatatgtt cggcagtttt ctgcgttatt tactgcgtcg
     1501 ccaaccgctg aatgcgttac ttgtcctgcc gttgttgccg atcatagcca ttgcgttatt
     1561 gataaaaggt cgtgcggcac gctggccgat gagtctgctt ctgtggggat gcacttttgg
     1621 tcacaatgaa gcgcgtttgc aggcgttgca ggccgatttc gtacgctggt ttcgtgacaa
     1681 tgttaccgcc tttccgctgg ttcaggagcg attaaccacc tacctgttaa gttccgatgc
     1741 tgatatctgg ttgattaccg gctctccgca gccgctggtt gaagcggttt atttcgatac
     1801 gccctggctg ccgcgggtta atcttatcgc cagccaaatt cagcgtggct atggtggttg
     1861 ggtattgacg atgcgttgtc tgggacatga aaaggtcgca caactggagc gcaaaatcgg
     1921 cacgccgctg cggctgtaca gtggctatag cgacagtaat caggacaatc cgctgcttta
     1981 tttctgtcag catcgttggc gagtaacccc gcgcggtgaa ctccagcaac tggaatagag
     2041 taaagcatag cgtccgtgta taatgcgccg cgcttttata accggagttt tctttttgtc
     2101 tgaagtcgaa tttagccacg aatactggat gcgtcacgcg ctgacgctgg caaagcgtgc
     2161 ctgggatgag cgggaagtgc cggtcggcgc ggtattagtg cataacaatc gtgttattgg
     2221 cgaaggctgg aaccgtccga ttggtcgaca tgatcccacc gcacatgcag aaatcatggc
     2281 cctgcggcag ggcggtctgg tgatgcaaaa ttatcgactg atcgacgcca cgctgtatgt
     2341 cacgcttgaa ccctgtgtaa tgtgtgccgg agcaatgatc cacagtcgca ttggtcgcgt
     2401 ggtctttggt gcgcgtgacg cgaaaactgg cgctgcggga tctttaatgg atgtgctgca
     2461 tcatccgggt atgaatcacc gggtggaaat taccgaagga atactggcgg atgagtgcgc
     2521 ggcgttgctc agtgacttct ttcgcatgcg ccgccaggaa attaaagcgc agaaaaaagc
     2581 gcaatcctcg acagattaat tttgtttctc ttcactccct tttctggaaa acagcagaga
     2641 gggagagtgg gtcaaatagt ttgatgacga ctgggaaaga aacgagagaa aaggaaattt
     2701 ctctttgccc agctccgtag gcgataccgc cggataatcc tgcgccagtt gcatcgccgc
     2761 ttctgtagcc tgcttctctt tctcctgcag ataacccacc aggctaatct gatacttacg
     2821 aatattttcg acataagcgt aggcttcatg cccacgagcg tagccgtaag tcagcttgct
     2881 gtaatagggt ttctggctaa gtaaaggcag acgctgtttt acgtcagccc aactgtcagg
     2941 attacctttg gtttttgccg tcagggcgcg ggcatccagc atatgcgcat agcccatatt
     3001 gtacgcggca agggcaaacc agatccgttc gttctccggc acactttccg gcactttact
     3061 catcatatcc tgcaaataac gcacgccgcc gctgatgctc tgttcggcat cggtacgatc
     3121 cgtaatgccg aggctttgcg cggtgttttt ggttaacatc atcatgccgc gcacacccgt
     3181 cggtgaagtg gcctgagcat cccagtgcga ttcttgataa gcaatagcgg ccagcaaacg
     3241 ccagtcaatt tcttcggcgt atttctcaaa caggggcttt aactgcggca gtaccgcatc
     3301 gacggcgcgt aaaaatgtgc gcgtatcgac gtaatcaaaa tcatcgccat gccccaggta
     3361 tttctcttca atgcgtgcca gcgtaccgtc ttcattcatc tcattgaaga aatcgagcag
     3421 agcggcggaa agggtattat cgccatctaa cgggctaaac caggtcaccg gttgttcatc
     3481 ggtgatatcg agcgctacgg cgagttccgg gtgaacgcgc tgaaacaggc tgatggcgac
     3541 agaatcagca atggtgtaat cgagttttcc ttcgatgaca tcttccatta attccgcaga
     3601 gccttttttg tcgtctacct tccagcttaa ttccgggaat tttgtttctt tcagggtctg
     3661 gagatcgtta accaccacat gacccggtgc aacggtgagt tgctccgccg tcaggttgcc
     3721 cagtgtacgt gggcgatact gacccacttt ataaaccagt tgttgtgaca cggaataata
     3781 ggtagggcca ggctgataat tttttacacg ctcactgtta tagaccaggc ctgccgccag
     3841 caggtcggcg ttaccattat cgaggtcgtc aaacagctgg ctgatattct gccgcacggt
     3901 cactttcagt tttacgccga ggtaatcggc aaactgtttc gccagttcgt aatccaggcc
     3961 aaaaggtttc ccgttgattt cgttataagt caggggggta tgaatggtgc tcacacgcaa
     4021 ctctccccgc gcttgaatgg cggcgatacg gttgtcggct ttaccaaacc agggaatgga
     4081 tggccagaga gcgaccgcga gcagcagtgc cagaatgccg atgaacagat aattaatctt
     4141 taattttttc aattagttaa ttctctgtgt cgtgcgcgtc ccagcttgaa aaaacgtaat
     4201 aatagtgaaa ggtttactca taaatgagcg gcattttgcg taaacctgcg ccagttggca
     4261 acttattaca gccattggcg gcacgcgttg ctaattcacg atggtgattt tatttccacg
     4321 caaacggttt cgtcagcgca tcagattctt tataatgacg cccgtttccc ccccttgggt
     4381 acaccgaaag cttagaagac gagagactta tgatggaaat tctgcgtggt tcgcctgcat
     4441 tgtcggcatt ccgaatcaac aaactgctgg cacgttttca ggctgccagg ctcccggttc
     4501 acactattta cgccgagtat gtccattttg ctgacctcaa tgcgccgtta aacgatgatg
     4561 agcacgcaca acttgaacgc ctgctgaaat atggcccggc actcgccagc cacgccccgc
     4621 aaggcaaact cctgctggtg accccgcgtc ctggcaccat ctctccctgg tcttcgaaag
     4681 cgaccgatat cgcccataat tgcggtctac aacaggtaaa ccgccttgag cgcggcgttg
     4741 cttactatgt agaagccggt acgctgacca atgaacaatg gcagcaggtt accgctgaac
     4801 tgcacgaccg catgatggaa acggtctttt ttgctttaga tgatgcagag cagctgtttg
     4861 cacaccatca accgactccg gtcaccagcg ttgatttgct ggggcagggc cgtcaggcgc
     4921 taatcgacgc taacctgcgt cttggcctgg cactggcgga agatgaaatt gactatttgc
     4981 aggatgcttt cacaaagctg ggtcgtaacc cgaacgacat cgaactgtat atgtttgccc
     5041 aggcgaactc cgagcactgc cgccacaaaa tttttaacgc cgactgggtt atcgatggtg
     5101 aacagcagcc gaaatcactg tttaagatga tcaaaaacac cttcgaaacc acgccggatc
     5161 acgttctctc tgcttataaa gataacgccg ccgtaatgga aggttctgaa gtgggccgct
     5221 actttgctga ccacgaaacg ggccgctacg atttccatca ggaacctgcg catatcctga
     5281 tgaaagttga aactcacaac cacccgacgg cgatttctcc gtggccgggc gcggcgaccg
     5341 gttccggcgg tgaaatccgc gatgaaggtg cctccgggcg cggcgcgaag ccgaaagctg
     5401 gtctggtcgg tttctccgtg tccaacctgc gaattcctgg cttcgaacag ccgtgggaag
     5461 aagatttcgg taaacctgag cgcattgtta ccgcgctgga catcatgacc gaaggcccgc
     5521 tgggcggtgc ggcgtttaac aacgaatttg gtcgtccggc actgaatggc tacttccgta
     5581 cttatgaaga aaaagtgaac agccacaatg gcgaagagct gcgcggttat cacaaaccga
     5641 tcatgctggc gggcgggatc ggcaacattc gtgccgatca cgtacaaaaa ggcgagatca
     5701 acgttggtgc gaagctggtc gttctcggcg gcccggcaat gaacatcggt cttggcggcg
     5761 gcgcggcgtc ttccatggcg tctggtcagt ctgatgccga cctcgacttc gcttccgtac
     5821 agcgcgacaa cccggagatg gagcgtcgct gccaggaagt gatcgaccgt tgctggcagc
     5881 ttggtgatgc caacccaatc ctgtttatcc acgacgttgg cgcaggcggt ctttctaacg
     5941 ccatgccgga actggtgagc gacggcgggc gcggcggtaa attcgaactg cgcgatatcc
     6001 tgagcgacga accgggcatg agcccgctgg aaatctggtg taacgaatcc caggaacgct
     6061 acgtgctggc agttgctgcc gatcagttac cgctgtttga cgaactgtgt aagcgtgagc
     6121 gcgcgccata cgcggtgatt ggtgaagcga ccgaagaact gcatctttct ttgcacgatc
     6181 gtcattttga taatcagccg atcgatctgc cgctggacgt actgcttggt aaaacgccga
     6241 agatgacccg cgaagtacaa acgctgaaag cgaagggtga tgcgctggtt cgtgaaggga
     6301 ttaccattgc tgacgcggtg aaacgcgtgc tgcacctgcc gaccgtggcg gagaaaactt
     6361 tcctggtgac cattggtgac cgcagcgtaa ccggcatggt ggcgcgcgat cagatggtgg
     6421 gtccgtggca ggtgccggtc gctaactgcg cggtcactac cgccagcctc gatagctact
     6481 acggtgaagc gatggcgatt ggcgagcgtg cgccggttgc gctgctggat ttcgccgctt
     6541 ctgcccgtct ggcggtcggt gaagcgttaa ccaacatcgc cgcaacacaa attggcgata
     6601 tcaaacgcat taaactttcc gccaactgga tggcggcggc aggccaccct ggtgaagatg
     6661 caggcctgta tgaagccgtt aaagccgtgg gtgaagaact ttgtccggcg ctgggcctga
     6721 cgatcccggt gggtaaagac tctatgtcga tgaaaacccg ttggcaggaa ggtaacgaag
     6781 agcgcgaaat gacgtcgccg ctgtcgctgg tgatttctgc atttgcccgc gtggaagatg
     6841 tacgtcacac catcacgcca cagctttcta ccgaagataa cgcactgctg ctgattgatt
     6901 tgggcaaagg caataacgcg ctgggcgcaa cggcgctggc gcaggtttat cgtcagcttg
     6961 gcgacaaacc ggcagatgta cgcgatgtcg cgcaactgaa aggcttctat gacgcgattc
     7021 aggcgctggt tgcacagcgt aagctgctgg cgtatcacga ccgctctgat ggcggcctgc
     7081 tggtaacgct ggcggaaatg gcctttgctg gtcattgcgg cattgacgcg gatatcgcct
     7141 ctctgggtga cgatcgcctg gcggcgttgt ttaacgaaga actgggtgcg gtgattcagg
     7201 ttcgtgccgc tgaccgtgaa gccgttgaag cggtactggc acagcatggg cttgcggatt
     7261 gcgtccatta tgtagggcag gcggtttccg gtgaccgttt tgtgattacc gccaacgggc
     7321 agactgtatt cagcgaaagc cgcactacgt tgcgtgtctg gtgggcagaa actacctggc
     7381 agatgcagcg cctgcgtgac aacccggagt gtgccgatca ggagcaccag gcgaaatcaa
     7441 acgacgccga tccgggcctg aatgtgaaac tgtcgttcga tatcaacgaa gatgtggcag
     7501 caccgtatat tgccactggt gcgcgtccga aagttgccgt gctgcgtgag cagggggtga
     7561 actcgcatgt tgaaatggcg gcagctttcc accgtgcagg cttcgatgca atcgatgtgc
     7621 atatgagcga cctgctggct ggacgcacgg gcctggagga tttccacgct ctggtagcgt
     7681 gcggtggatt ctcctacggt gacgtgctgg gtgctggtga aggctgggcg aagtctatcc
     7741 tgttcaatga ccgtgtacgc gatgagtttg caaccttctt ccaccgtccg caaacgctgg
     7801 cgctgggggt atgtaacggt tgccagatga tgtctaacct acgcgaactg atcccaggta
     7861 gtgagttgtg gccacgtttt gtgcgcaata cctccgatcg ctttgaagcg cgtttcagcc
     7921 tggttgaagt aacccaaagc ccgtctctgc tgttgcaggg gatggtgggc tcgcaaatgc
     7981 cgattgctgt ctctcatggc gaagggcgcg tggaagtgcg tgatgcggcg catctggcgg
     8041 cactggaaag caaagggctg gtggcactgc gctatgtcga taacttcggc aaagtcactg
     8101 aaacctaccc ggctaacccg aacggttctc cgaatggtat tacggcagtc acgactgaaa
     8161 gtggtcgagt caccattatg atgccgcacc cggaacgtgt tttccgtact gtcagcaact
     8221 cctggcatcc ggaaaactgg ggcgaggatg ggccgtggat gcgtattttc cgcaatgcac
     8281 gtaagcagtt ggggtaagcc gtcaacccat tggttttgca gccggatgag gcattaagca
     8341 ccaactacta ttataagctc ccaacttcgg gagctttttt gtgtctgtaa atcacgacaa
     8401 tgggtggttt accgcgtcgc tttctggcga cacttaacta attgatttta atggcatcta
     8461 ataagtttat cttaagttgt ctctttttag cgacacagtg gctgatttac agccaaatag
     8521 cgaagaagcc ataacccctt caaaacagcg tacaatcagt acgttaccaa actattttct
     8581 ttattggcac agttactgca taatagtaac cagtggctca ttcaccgact tatgtcagcc
     8641 ccttcgggac gtgctacata aaatacgaat gacgcacaac aaggtgcctg ccgtccaact
     8701 tctgatatca gcgtagctat atcaaccatc gggcgaaacg tcgagttagg caccgcctta
     8761 ttccataaca aagccgggta attcccggct ttgttgtatc tgaacttccc ctcggttagc
     8821 atcaggctat tcgcgtctga cgagagtaac accttgaaac gctggcccgt ttttccccgc
     8881 tcattacgac aactggtaat gctggcattt ttgctgattc tgctgcccct gttggtgctg
     8941 gcatggcaag cctggcaaag cctgaatgcg cttagcgatc aggcggcgct ggttaaccgc
     9001 actacgctta ttgatgcccg gcgtagtgaa gcgatgacca acgcggcgct ggagatggag
     9061 cgtagctacc gtcagtattg cgtgctggac gaccctacgc ttgcgaaggt ttatcaaagc
     9121 cagcgcaagc gttacagcga aatgctcgat gcccacgcag gcgtactgcc agacgataaa
     9181 ctttaccagg cattgcgtca ggatttgaac aatctggccc aacttcagtg taacaacagt
     9241 ggtcccgatg ctgccgccgc cgcgcgtctt gaagcctttg ccagtgccaa taccgaaatg
     9301 gtgcaggcca cgcgcacagt ggtgttctct cgtgggcagc aacttcagcg tgaaatcgcc
     9361 gaacgtgggc aatattttgg ttggcaatcg ctggtgctat ttctggtgag tctggtgatg
     9421 gtgctacttt tcacgcggat gattatcggg ccggtgaaaa atatcgagcg catgatcaac
     9481 cgtctggggg aagggcgttc tctgggcaat agcgtttcgt tcagtggacc gagcgagtta
     9541 cgctcggttg ggcaacgtat tctttggtta agtgagcgcc tgtcatggct ggaatcccaa
     9601 cgccatcaat ttttaagaca tttatctcat gaattaaaaa cgccactggc gagtatgcgc
     9661 gagggcactg aattactggc tgaccaggtt gtcgggccgc ttacgccaga gcaaaaagag
     9721 gtggtgagca ttcttgatag cagcagccgc aatttgcaaa aactgatcga acaactgctt
     9781 gattacaacc gtaaacaggc ggacagtgcg gtggaactgg agaatgttga attagcaccg
     9841 ctggtggaga cagtggtttc tgctcatagc ctgcccgcac gggctaaaat gatgcatacc
     9901 gacgtcgatc tcaaagcaac agcttgcctg gcggagccaa tgctgctgat gagcgtactg
     9961 gataatcttt actccaatgc ggtgcactac ggggctgaat ccggtaacat ttgccttcgc
    10021 agcagttcac atggtgcgcg ggtttatatt gatgtcatca atacaggcac gcccattccg
    10081 caagaggaac gcgccatgat cttcgaaccc ttttttcagg gaagccacca gcgaaaaggg
    10141 gcggtgaagg gcagcggtct gggattaagc attgccaggg attgtattcg ccgtatgcaa
    10201 ggggaactgt atctggtcga cgagagcggg caagacgttt gtttccgcat tgatttaccg
    10261 tcgtcgaaaa acacgaaata atgctaacag cccatcccat caggctattt tatttgtcag
    10321 ttcggccccg ggcagtgctc gaaatcttca cgtacgacat gtacgctctg gtttctccgc
    10381 gctgtccatg tccaaactgc ctgcaacaat tacgcccact gggataggct ttaagtctgg
    10441 tgaatatgcg acacattttt caacgattat tgccccgacg gctgtggatg gcagggctgc
    10501 catgtctggc attgctgggt tgcgtgcaga gtcacaataa gccagccatt gatacgccag
    10561 cagaagaaaa aattccggtt tatcaactgg ctgactatct ttctactgaa tgtagcgaca
    10621 tctgggcgct gcaaggtaaa tcaaccgaaa ccaatccgct ttactggctg cgggcgatgg
    10681 attgtgctga tcgtttaatg cctgcgcagt cgcgtcagca agcccgccaa tatgacgacg
    10741 gcaactggca aaataccttt aaacaaggga ttttgctcgc cgacgccaaa attacgccgt
    10801 atgagcgccg ccagctggtc gcgcgcattg atgcgctaag taccgagatc ccggcacagg
    10861 ttcgccctct gtatcaactc tggcgcgacg gtcaggcgct gcaactgcaa ctggcggaag
    10921 agcgccaacg ctacagtaaa ctccagcagt ccagcgacag cgagctggat actttgcgcc
    10981 agcaacatca cgttctacaa cagcaactgg aactcactac ccgcaagctg gaaaacctga
    11041 ccgatattga acgccagctc tcgacccgca aaccggctgg aaatttctcg ccggatacgc
    11101 cgcacgagag tgaaaagccc gcgccatcca ctgatgaggt cactcctgat gagccataaa
    11161 cctgcgcatt tattactggt cgatgacgat ccgggattgc tgaaactgct tggcctgcgc
    11221 ctgaccagcg aaggctacag cgtggtcacg gcggaaagtg gtgctgaagg attacgggta
    11281 ctgaatcgcg aaaaagtaga tttagttatc agcgacctgc ggatggatga aatggatggt
    11341 atgcagctgt ttgctgaaat ccagaaagtg cagccgggaa tgccggtaat tattcttacc
    11401 gcacatggtt ctattcccga tgccgttgct gcaacacagc agggagtgtt cagtttcctc
    11461 accaagcctg tcgataaaga cgcgctatat caggcaattg acgatgcgct ggaacaatcc
    11521 gcgccagcca ccgatgaacg ctggcgcgag gcgattgtca cccgcagccc gctgatgctg
    11581 cgtttgctgg aacaggcgcg gctggtggcg caatcagacg ttagcgtttt gattaacggt
    11641 cagagcggca ccgggaaaga gattttcgcc caggctatcc acaacgccag cccgcgcaat
    11701 agcaaaccat ttattgctat taactgtggt gcattgcccg agcaattgct ggagtcggag
    11761 ctgtttggtc atgcgcgtgg cgcgtttact ggcgctgtca gcaatcgcga aggtttattc
    11821 caggcggcgg aaggcggtac gctatttctc gatgagattg gcgatatgcc tgcaccgttg
    11881 caggtcaaac tgctgcgtgt gttgcaggag cgtaaagtgc gcccgctggg cagtaaccgc
    11941 gatattgata tcgacgtgcg gattatttcc gccactcacc gcgacctgcc aaaagcgatg
    12001 gcgcgcgggg aattccgtga agatctctat taccgcctca acgttgtcag cctgaaaatt
    12061 ccggctctgg cagagcgcac agaagacatt ccgctactgg caaatcacct gttgcgccag
    12121 gcggcagagc gacataaacc gtttgtccgc gcgttctcca ccgatgcgat gaaacgcctg
    12181 atgaccgcta gctggccggg taatgtacgc cagttggtca acgtgattga acagtgcgtg
    12241 gcgctgacct catctccggt gattagcgat gcattagtag agcaggcgct ggagggcgaa
    12301 aacacggcgc tgccaacctt tgttgaggca cgtaatcagt ttgaactcaa ctatttgcgt
    12361 aagctgctgc aaatcaccaa aggcaacgtc acccacgcgg cgagaatggc ggggcgcaac
    12421 cggacagaat tttataaact gctttcccga cacgagctgg atgcaaacga tttcaaggaa
    12481 tgaattggcg ttatgtgtta cgtttagcag atcaaaagac aggcgacctt ttcaaggaat
    12541 agcatgaaaa agattgatgc gattattaaa cccttcaagc tggacgatgt tcgcgaagca
    12601 ctggctgaag tcggcattac cggcatgacg gtcactgagg tgaaaggttt tggccgccag
    12661 aaaggccata ccgagctgta ccgcggcgcg gagtatatgg tggattttct gccgaaagtg
    12721 aaaattgaga ttgtcgtacc ggacgacatt gtcgatacct gtgtcgatac cattattcgc
    12781 acggcgcaaa ccggcaaaat cggtgacggt aaaatcttcg tctttgacgt ggcacgggtc
    12841 attcgtatcc gtaccggtga agaagacgac gcggcaattt aaaccgcgtc aagggttgcc
    12901 ggatgtttcc atccggcaac atcaaatcac agcaccttat gcgggccaaa gcattcgtaa
    12961 tgaatatttt cctgcttcac gcccagatcc actaactgtt tcgcggcaaa ctgcatgaag
    13021 ccaaccgggc cgcagagata gaactgcatt gtcggatcgc tgaacgcacc ttccagtttg
    13081 ctcaaatcca tcagaccttc gctatcaaac tgacctttag cgcgatcggc ttcgctcggc
    13141 tgacgatacc aggtgtgcgc ggtaaagcgc ggcagtgaca gccccagttc cttaacttca
    13201 tcagcaaagg cgtgaacatc gccattttct gccgcatgga accagttcac ttgtgctgtg
    13261 tggcctgctt ttgccagtgt gtcgagcatt gccagcattg gcgtttgacc gacaccggca
    13321 gagattaacg tcactggtgt gtcatctgcg acagccataa aaaaatcacc tgctggagcg
    13381 accagtttca cgacatcgcc aacattggcg tgattgtgca gccagttgga tacctgccca
    13441 ccctcttcgc gtttcaccgc aatacgatag cctttgccat ccggtttgcg agtcaaagag
    13501 tactgacgaa tttcctgatg cgggaaacct tccggcttca gccagacgcc gagatattgc
    13561 cccggacggt attctgccac tgcgccaccg tcgaccggct ccagttcgaa gctggtgata
    13621 agcgcgctgc gcggtgtttt agccacaatg cggaaatcgc gagtaccttc ccagccaccg
    13681 gctttgctgg cgttttcgtt atagatttcc gcctcgcgat tgataaatac attagccagt
    13741 acgccatagg ctttacccca cgcgtccagc acttcctggc ccgggctgaa catttcgtcc
    13801 agcgtcgcca acaggtgttc accgacaatg ttgtactgtt ccggtttgat ctggaagctg
    13861 gtgtgcttct gcgcgatttt ttctaccgct ggcagcagcg caggcaggtt ttcaatatta
    13921 ctggcgtagg cggcaatagc gttaaacagg gcttcacgtt gatcgccatt gcgctggtta
    13981 ctcatgttaa aaatttcttt gagttctggg ttatgagtaa acatacggtc gtagaaatgg
    14041 gcggttaact ttggtcccgt ttccaccagt aaagggatgg tggcttttac tgtagcgatg
    14101 gtttgagcgt caagcatatg gtcttccttt tttttgcatc ttaattgatg tatctcaaat
    14161 gcatcttata aaaaatagcc ctgcaatgta aatggttctt tggtgttttt cagaaagaat
    14221 gtgatgaagt gaaaaatttg catcacaaac ctgaaaagaa atccgtttcc ggttgcaagc
    14281 tctttattct ccaaagcctt gcgtagcctg aaggtaatcg tttgcgtaaa ttcctttgtc
    14341 aagacctgtt atcacacaat gattcggtta tactgttcgc cgttgtccaa caggaccgcc
    14401 tataaaggcc aaaaatttta ttgttagctg agtcaggaga tgcggatgtt aaagcgtgaa
    14461 atgaacattg ccgattatga tgccgaactg tggcaggcta tggagcagga aaaagtacgt
    14521 caggaagagc acatcgaact gatcgcctcc gaaaactaca ccagcccgcg cgtaatgcag
    14581 gcgcagggtt ctcagctgac caacaaatat gctgaaggtt atccgggcaa acgctactac
    14641 ggcggttgcg agtatgttga tatcgttgaa caactggcga tcgatcgtgc gaaagaactg
    14701 ttcggtgctg actacgctaa cgtccagccg cactccggct cccaggctaa ctttgcggtg
    14761 tacaccgcgc tgctggaacc aggtgatacc gttctgggta tgaacctggc gcatggcggt
    14821 cacctgactc acggttctcc ggttaacttc tccggtaaac tgtacaacat cgttccttac
    14881 ggtatcgatg ctagcggtca tatcgactac gccgatctgg aaaaacaagc caaagaacac
    14941 aaaccgaaaa tgattatcgg tggcttctct gcatattccg gcgtggtgga ctgggcgaaa
    15001 atgcgtgaaa tcgctgacag catcggtgct tacctgttcg ttgatatggc gcacgttgcg
    15061 ggcctggttg cagctggcgt ctacccgaac ccggttcctc atgctcacgt tgttactacc
    15121 accactcaca aaaccctggc gggtccgcgc ggcggcctga tcctggcgaa aggtggtagc
    15181 gaagagctgt acaaaaaact gaactctgcg gttttccctg gtggtcaggg tggtccgttg
    15241 atgcacgtaa tcgccggtaa agcggttgct ctgaaagaag cgatggagcc tgagttcaaa
    15301 acttaccagc agcaggtcgc taaaaacgct aaagcgatgg tagaagtgtt cctggaacgc
    15361 ggctacaaag tggtttccgg cggcactgat aaccatctgt tcctggttga tctggttgat
    15421 aaaaacctga ccggtaaaga agctgacgcc gctctgggcc gtgctaacat caccgtcaac
    15481 aaaaacagcg taccgaacga tccgaagagc ccgtttgtga cctccggtat tcgcgtgggt
    15541 actccggcga ttacgcgtcg cggcttcaaa gaagcagaag cgaaagaact ggctggctgg
    15601 atgtgtgacg tgctggacag catcaatgat gaagccgtta tcgagcgcat caaaggtaaa
    15661 gttctcgaca tctgtgcacg ttacccggtt tacgcataag cgaaacggtt aatctgttga
    15721 aaaaggcttc cttcgggaag cctttctacg ttatcgcgcc atcaaatctg tcgtaactgc
    15781 gcctcaacat acaaatagcc aatccccagc acctgttgtg cgcggcttaa ttgcccaaag
    15841 ccaattttcg tcgctttcac gctcggtcct tcgtcgcggt cgaacgggtt aaatcccgtc
    15901 tggcgaataa tggtattaag ccagtgctca ccaaaggcac aactgcgacc gtacaaccaa
    15961 atctgattaa tattgaggat gttgaggaag ttatacagac tcaaaccaat ggcattggca
    16021 gagcgatcaa cccagctggt gatccacggt tctccactct gccaggcagc gattaactgc
    16081 gctgttgtca gtttttcagg atcaagctga gtattaaccg gttgtgattt tagccatacc
    16141 cgcgcctgtt tttttaatgc acttaacgag gcgacggttt ccaggcagcc gtaacgtcca
    16201 cagtcgcaga cgacgccatc cggattaacg atggtgtgac caatctgtcc gctaccatac
    16261 aaacagccgc gataaatttg ctcgttaatc acgaacgacg agccaatgcc gtaatcaacg
    16321 ttgatcacgc agaaatcccg ttcctgcgaa ttattttgcc atttctccgc cagcgccagc
    16381 atcacgcagt cattatcgac catcacccga atgccgagct tctcttccag cagatacttt
    16441 acctcaaccg gcgttgtcca cggcgcttgc ggcatggttt gcgacacgcc ggtcaccgga
    16501 tcaacctgac cgtggattgc cagcgccagg ttgatggtac ggtccggcca caatttacgg
    16561 tggcgatgcc agcatttttc gatttcggac agcagcgcct gcggcgtcgg tgcatcaatc
    16621 tgtaaatact caaattcacc tttcggactt aaacaagcat tagcaacctg acactcaata
    16681 ctggtgggcg tcacgttcag gcacaacgtc cagtcacctt ccggcgcaat cagccatgta
    16741 ccgctactat gcccgcgcgt ctggctttca tcgtcgatat tcaccacccg tttttcgctt
    16801 tccagttcct gcaaaatatt actgaccgcc ggaatcgaaa tttgcgccag ccgggccagc
    16861 gttgatttat tggcgcgctt ttgccggtac agcagttcca gaatcacgca tttgttatgg
    16921 tggcgaatct gttgattatt aatgcaggct ctcatcttca ctaaaccttg tttagttaat
    16981 tgcgtgatta ttccgcgaaa acattgccag attgttcaat acactgccac aaatctttta
    17041 attcagtatg tcttgttaat attgagggca ccatgactcc agtaaaagtg tggcaagagc
    17101 gcgttgagat cccgacctat gaaaccgggc cgcaggatat acatcccatg ttcctggaaa
    17161 atcgcgttta tcagggatcg tccggcgcgg tttatcccta cggcgtgacc gatacgctga
    17221 gcgagcagaa aaccctgaaa tcctggcagg cggtgtggct ggaaaacgac tacatcaaag
    17281 tgatgatcct gccggagctg ggcggtcggg tgcatcgcgc gtgggataaa gtgaaacagc
    17341 gcgattttgt ctatcataat gaagtcatta aaccggcgct ggtggggttg ctggggccgt
    17401 ggatctccgg cgggattgag tttaactggc cgcaacacca tcgcccgacc acctttatgc
    17461 ccgtcgattt caccctcgaa gcccatgaag acggcgcaca aaccgtgtgg gtcggcgaaa
    17521 cggagccgat gcacggttta caggtgatga caggtttcac cctgcgccct gaccgggcgg
    17581 cactggaaat tgccagccgc gtctataacg gcaacgccac gccgcgtcat ttcttgtggt
    17641 gggccaaccc ggcagtgaaa gggggtgaag ggcatcagag cgtcttcccg ccggatgtaa
    17701 cggcggtgtt tgatcacggc aaacgggccg tctccgcttt ccccatcgcc accggcactt
    17761 actacaaagt ggactactcc gccggagtgg acatttctcg ctataaaaat gtgcccgttc
    17821 caacctcata tatggctgaa aaatcacagt acgattttgt tggcgcgtgg tgtcacgatg
    17881 aagatggcgg tttgctgcac gttgccaacc accatattgc gccaggtaaa aaacagtgga
    17941 gctggggaca cagtgaattt ggccaggcgt gggataagag tctgaccgac aataacggcc
    18001 cgtatatcga actgatgact ggtatttttg ccgataacca gcccgatttt acctggcttg
    18061 atgcttacga agagaagcgt tttgagcagt atttcctgcc ttaccattct ttgggcatgg
    18121 tgcaaaacgc ctcccgcgat gcggtgatta aactccagcg tagcgagcgg gggattgagt
    18181 gggggctgta tgccatctct ccgttgaaag gataccgtct ggcgatccgc gaaatcggca
    18241 aatgcaacgc gttgctcgat gatgccgtgg ccctgacacc agcgaccgcc atccagggcg
    18301 tgttgcacgg tatcaatcct gaaaggctga ccattgaact ctccgatgcc gacggcaata
    18361 tcacactgag ttatcaggaa catcagccgc aagagttgcc gttgccggac gtcgccaaag
    18421 cgccactggc agcacaagac attaccagta cagatgaagc ctggtttatc ggtcagcatc
    18481 tggagcaata tcatcacgcc agccgttcac cgttcgatta ctacctgcgc ggcgtggcgc
    18541 tggatccgct ggattatcgc tgtaacctgg cgctggcgat gctggaatat aaccgagcag
    18601 atttcccgca agcggtggcg tatgccactc aggcgctgaa acgcgcacat gcgctaaaca
    18661 aaaatccgca gtgcggacag gcgagtttga ttcgcgccag tgcttacgaa cgtcaggggc
    18721 aatatcaaca agccgaagag gatttctggc gggcggtctg gagcggcaac agtaaagcag
    18781 gtggctatta tggtctggca cggctggcgg cgcgtaatgg tcacttcgac gctggtctgg
    18841 atttttgcca acaaagtctt cgcgcctgcc caaccaatca ggaagtgctt tgcctgcata
    18901 atctgctgct ggtgttaagt ggtcgtcagg acaacgcgcg tttgcagcgc gagaaactgc
    18961 tgcgtgatta tccgctgaac gccactctgt ggtggctgaa ctggttcgat ggtcgtagcg
    19021 aatcagccct cgcgcagtgg cgcggtctgt gtcagggacg cgacgttaac gccctgatga
    19081 ccgccgggca actgattaac tggggaatgc ccaccctggc ggcagagatg ctgaacgcac
    19141 tggactgcca gcgcacgctg ccgctttact tgcaagccag cttgctgccg aaagctgaac
    19201 gcggcgaact ggtcacaaaa gccattgatg tcttcccaca gtttgtccgt ttcccgaata
    19261 cgctggaaga agtggcggcg ctggagagta ttgaagagtg ctggtttgcc cgtcatttac
    19321 tggcctgctt ctactacaac aagtgcagct acaacaaagc cattacttta tggcaacgtt
    19381 gcgtagagat gtcgccggag tttgccgacg gctggcgcgg gttagcgatc catgcgtgga
    19441 ataagcaaca cgattatgag ctggccgcgc gttatcttga taatgcttat cagcttgcgc
    19501 cgcaggatgc acgtctgctt ttcgaacggg atttgctgga taagttaagc ggtgtcacac
    19561 cggagaaacg actggcgcgt ctggaaaata atctggaaat tgcgctgaaa cgcgacgaca
    19621 tgaccgcaga actgctcaat ttgtggcatc tcacgggtca ggcagacaaa gcggcggaca
    19681 ttctcgccac gcgcaaattc cacccgtggg aaggcgggga agggaagatc accagtcagt
    19741 ttatcctcaa ccagttatta cgcgcctggc agcatcttga tgccagagag ccgcagcagg
    19801 ccagcgaact gcttcatgcc gcgttgcatt atccggagaa tttaagcgaa ggccgtttac
    19861 cggggcaaac tgacaacgac atctggttct ggcaggcgat atgcgccaac gcgcagggcg
    19921 atgaaactga agcgacgcgt tgtttacgtc tggcggcgac tggcgatcgc accattaaca
    19981 ttcacagtta ttacaacgat cagccggttg attatctctt ctggcaagga atggcgctgc
    20041 gactgctggg cgaacaacac accgcacagc aactgtttag tgaaatgaaa cagtgggcgc
    20101 aagagatggc gaaaaccagt atcgaggcgg atttctttgc tgtttcacaa cctgacctgt
    20161 tgtcgctgta tggcgattta caacagcagc ataaagaaaa atgcctgatg gtggcgatgc
    20221 tggcgtccgc gggactcggg gaggttgcgc actatgaatc tgctcgcgct gaattgatgg
    20281 cgattaatcc agcctggccg aaagcggcat tattcaccac cgtgatgcct tttattttta
    20341 gctacgttca ctaaacctta ttaactgtaa tatttgagcg gcacgcgctg ccgctcacgc
    20401 tcaaataatt aaaataagcc gttgtaaata aaaaccctac aggaggcact atgcctaaaa
    20461 aaatgacaag aacccgtaat ttattattaa tggctacgct attgggtagc gcactatttg
    20521 ccagggctgc ggataaagaa atgactattg gcgcaatata ccttgatacc cagggatatt
    20581 acgccggagt gcgtcagggc gttcaggatg cggcaaaaga ttcttcagta caggtacagt
    20641 taattgaaac taacgctcag ggcgatattt cgaaagaaag tacctttgtt gataccctcg
    20701 tggcgcgtaa tgtcgatgcc attattttat cggcagtgtc tgaaaatggc agtagtcgta
    20761 ccgttcgtcg cgccagtgaa gcgggtattc cggtgatttg ctacaacacc tgtattaatc
    20821 aaaaaggtgt cgataaatat gtctcggcgt atctggtcgg cgatccgctg gaatttggta
    20881 aaaaactggg taacgctgcc gccgattatt ttattgccaa taaaattgac cagccgaaaa
    20941 ttgccgtcat caattgcgaa gcctttgaag tttgtgtgca gcgacgtaaa ggatttgaag
    21001 aggtattaaa atcccgcgtt cccggcgcgc aaatagtcgc taatcaggaa gggactgttt
    21061 tagataaagc gatttccgtt ggtgaaaaac taattatctc cacgccggat ctcaacgcca
    21121 ttatggggga atcgggcggt gcgacactcg gcgcggtaaa agcggtacgt aatcaaaatc
    21181 aggccggaaa aattgctgtt ttcggttcgg atatgaccac tgaaattgct caggagctgg
    21241 aaaacaatca ggtgctgaaa gcggtggtgg atatttccgg taagaaaatg ggcaatgctg
    21301 ttttcgcgca aactttgaag gttatcaata aacaagccga cggtgaaaaa gtgattcagg
    21361 tgccaatcga tctctatacc aaaacggaag acggtaaaca gtggctggca acgcacgttg
    21421 atggtctgcc ctaatcgctg attcgcagag gtccctatgt tcacagcaac agaggcagtc
    21481 ccggtagcaa aagtggtggc aggaaataag cgttatcccg gcgtcgttgc gctggataac
    21541 gttaacttca cgctcaataa aggcgaagtt cgtgcgctgt taggcaaaaa cggtgcggga
    21601 aaatcgaccc tcattcgaat gcttaccggt agcgaacgtc cggatagcgg tgatatctgg
    21661 attggcgaga cgcgactgga aggtgacgaa gctacgctga ctcgccgtgc cgctgaactg
    21721 ggggtacgcg cggtttatca ggagttaagt ctggtggaag ggctgacagt ggcggaaaac
    21781 ctctgcctcg gtcagtggcc ccgccgcaac ggcatgattg attacctgca aatggcgcag
    21841 gacgcccaac gttgcttgca ggcgttgggc gttgacgtta gtcctgaaca acttgtttca
    21901 acactaagcc cggcgcaaaa gcagctggtg gaaattgcgc gggtgatgaa gggcgagccg
    21961 cgcgtggtca ttcttgatga acccaccagc tcgcttgcca gtgcggaggt tgaactggtg
    22021 atcagcgcgg tgaaaaagat gtcggcactg ggcgtggcgg tgatttatgt cagccaccgg
    22081 atggaagaaa ttcgccgcat tgcctcctgt gccaccgtta tgcgcgatgg tcaggtggcg
    22141 ggcgatgtga tgctcgaaaa cacctccacg catcatattg tgtcgctaat gctcgggcgc
    22201 gatcacgttg atattgcgcc ggtttcccct caggaaattg tcgatcaggc cgtgctggaa
    22261 gtccgtgcgt tacgccataa gcccaagctg gaggatatca gctttacgct ccgtcgtggc
    22321 gaagtactcg gcattgctgg cctgctgggg gcagggcgca gtgaattgtt gaaagccata
    22381 gttgggctgg agacgtatga acagggcgaa attgttatca acggcgagaa aatcactcgc
    22441 cccgattacg gcgacatgct gaaacgcggc attggctata cgccagaaaa ccgcaaagat
    22501 gcggggatca ttccctggct gggcgttgac gaaaatacag tgctgaccaa tcggcaaaaa
    22561 atcagcgcca acggtgtgct gcaatggtcc accatccgcc gcctgaccga agaggtgatg
    22621 cagcggatga cggtcaaggc cgccagtagc gaaacgccca tcggcacgct ttctggcggc
    22681 aatcagcaaa aagtggtgat cggtcgttgg gtctatgccg ccagccagat tttgttgctc
    22741 gacgagccaa cgcgtggcgt cgatatcgaa gccaaacagc agatttaccg tattgtccgc
    22801 gagctggctg ccgaaggaaa aagcgtggtg tttatctcca gtgaagtgga ggagttgccg
    22861 ctggtgtgcg accgcattct gttgttacag cacggtacgt tctcgcagga gtttcacgct
    22921 ccggtcaatg tggatgagct gatgtccgcc attctgtctg tgcactgatg aattttaccg
    22981 aggaacctga agatgtctgc ttcgtcatta ctattgccgc agggcaagag cgtctcgctc
    23041 aaacaatttg tcagtcgcca tattaatgag atcggtttgc tggtggtgat cgccattctt
    23101 tatctggtct tctctctgaa cgcgcctggc tttatctcat tgaataacca gatgaacgtg
    23161 ctgcgcgatg ccgccaccat cgggatcgcc gcatgggcga tgacgctgat tattatctcc
    23221 ggtgaaattg atgtcagcgt tggtccgatg gtggcttttg tctcggtgtg cctggcattt
    23281 ttgctgcaat ttgaagttcc gctggcggtg gcgtgtctgc tggtgttgct gttaggcgcg
    23341 ctgatgggaa cgctcgccgg ggtgctgcgc ggcgtgttta acgtgccaag ttttgttgcc
    23401 acgctgggtt tatggagcgc cctgcgcgga atggggctgt ttatgaccaa cgccttgcca
    23461 gtgccgattg atgaaaacga agtgctggac tggctggggg gacaatttct cggtgtgccg
    23521 gtatccgcgc tgatcatgat ggtattgttt gcgctgtttg tgttcatcag ccgcaaaacc
    23581 gccttcgggc gctcggtttt tgccgttggc ggtaatgcca ctgcagcaca gttgtgcggt
    23641 attaacgttc gtcgggtacg cattcttatc tttacccttt cgggattatt agcggcggtg
    23701 accggcattt tgttggcggc gcgcctcggt tccggtaacg caggtgccgc aaacggtctg
    23761 gagtttgacg tcatcgccgc ggtcgtggtt ggcggtacgg cgctttccgg tggtcgcggc
    23821 tcgttgttcg gtacgctgct tggcgtactg gtgattacgc taatcggtaa cggtctggtg
    23881 ctgctcggga ttaactcctt tttccagcag gtggtgcgcg gcgtcatcat cgtggtggcg
    23941 gtgctggcga atatcttgct gactcagcgt agcagtaaag cgaaacgcta aaccttaacg
    24001 cccctggcgc gggtgcggca gggatgaaaa ttaatcgtat tgttaatgag ggatttatga
    24061 aaacgatgct ggcagcttat ttaccaggaa attcgaccgt cgatctgcgg gaagttgcgg
    24121 tgccgacgcc ggggattaac caggtactga tcaaaatgaa atcctccggg atttgcggaa
    24181 gcgatgtcca ctatatctat catcaacacc gtgccacggc agcggcaccc gataaaccgt
    24241 tataccaggg atttatcaac ggtcatgaac cgtgcgggca gattgtggcg atggggcaag
    24301 gctgccgcca ttttaaagag ggcgaccgtg tgctggtgta tcacatttct ggctgtggtt
    24361 tttgcccgaa ctgccgtcgc ggctttccta tctcttgtac tggcgaagga aaagcggctt
    24421 acggctggca gcgtgacggc ggtcatgccg aatatctgct ggcggaagaa aaagatctga
    24481 tcctcctgcc ggatgcactg agttacgaag atggtgcgtt tatcagttgc ggcgttggta
    24541 cagcgtatga agggattttg cgcggcgaag tttccggcag tgataacgta ctggtggtcg
    24601 gtctggggcc ggtcggcatg atggcgatga tgctggcgaa aggtcgcggt gcaaaaagga
    24661 tcatcggcgt tgatatgctg ccggaacgtc tggcgatggc gaaacagtta ggagtgatgg
    24721 atcacggcta tttagcgacc accgaaggtc tgccgcagat aatcgccgaa ctcactcacg
    24781 gcggcgcgga tgttgcgctt gattgttccg gtaatgccgc aggtcgctta ctggcactgc
    24841 aatccaccgc tgactgggga cgcgtggttt acattggtga aaccggaaaa gtcgagttcg
    24901 aggttagcgc cgacctgatg caccatcaac ggcggattat cggctcctgg gtgaccagtc
    24961 tgttccatat ggaaaaatgc gcccacgatt taacggactg gaaactgtgg ccgcgtaacg
    25021 ccattaccca ccgcttctcg ctggaacagg cgggagatgc ttatgcactg atggcaagcg
    25081 gcaaatgcgg gaaagttgtg attaacttcc cggattaagg agttagcgga tgaccatcta
    25141 taccttatcc cacgggtcgc taaagctggg cgtttctgac cagggcggcg taatcgaagg
    25201 cttctggcgc gatacgacgc cgctcttacg tcctggtaaa aaaaacggtg tggcaaccga
    25261 tgcctcttgt tttccgctgg tgccatttgc aaaccgggtg agcggcaacc gttttgtctg
    25321 gcaggggcgt gagtatcaac tgcaaccgaa tgttgagtgg gatgcgcact atctgcacgg
    25381 cgacggctgg ctggggcagt ggcaatgtgt ctcgcgcaat gatgatatct tgtgtctggt
    25441 gtatgaacat cgcagcggtg tttatcacta tcgggtaagc cagacgtttc atttaacggc
    25501 ggatacgctg acggtgacac tctctgtcac caatcaaggg gcagagacgc tgccatttgg
    25561 taccggctgg catccttatt ttccgttgtc gccgcaaacg cggattcagg cacaggcgag
    25621 cggttactgg ctggagcggg agcagtggct ggcgggtgag ttttgcgagc agctaccgca
    25681 agaattggat tttaaccagc tcgcgccgtt gccgcgccag tgggtgaaca atggttttgc
    25741 cggatggaat ggtcaggccc gcatcgagca gccgcaggag ggttatgcca tcatcataga
    25801 aacgacgcca cctgcaccgt gttatttcat ctttgtttcc gacccggcgt ttgataaagg
    25861 atatgcgttt gatttcttct gtctggaacc gatgagccat gcgccagatg accatcatcg
    25921 cccggaaggt ggtgacctca ttgcgcttgc accaggggaa tcaacaactt cagagatgtc
    25981 gttgcgggtt gcgttactgt aatttataaa atgccggata aggcgttcac accgcatccg
    26041 gcactcggcg cacgatgcct gatgcgacgc ttgccgcgtc ttatcaggcc tacaattggt
    26101 gttggaacgg taggcaggat aaggcgttca cgccgcatcc ggcatctttc ctaacgccga
    26161 tcgagagaaa tcgcccccgg cccggtaatt gccaacagca ggaatgcgcc agcgatactg
    26221 acattcttcc agaaattaat catatttggc ccaaccgcat cgccggtcat atcccagtaa
    26281 tggtgaccaa tcaccgccgt acccagcgtg tagaaaataa acagcaccgc cagcggacgg
    26341 gtgaaaaagc caagcacgat taatatcgcg gcgggcactt ccataactac cgcaataatc
    26401 gctgccagca tcggcatcgg cgcgcccagc gaggccatat attggaccgt accgtcaaag
    26461 cccatcattt tgggaaaacc aaaaataatg aaaattaaga ccacggcgat acgggcaatt
    26521 aataacaaaa cggggcgtgc agcaccaaaa tcaaaataac gtagtgagtt cataaccaat
    26581 ccctttgcaa gataatgtga tttaaacgta atacacactc ttgcacctcg ccacgcccct
    26641 ggcattgaga tattcgctaa ttatcctggt tacagtgatt taagcgcgat gtcctcatct
    26701 atcagcagtt tcgcatcaaa cgttttgccg ctctggatcc atttgcggat taagcgaatc
    26761 tcacgcccct gattcagcgt taccgcaccg ataagcacgc cgttttgcag attaaaccaa
    26821 atcgccttct gagtttccgg gttgccacga caaatccagt catcgccatg catatcgcca
    26881 ataaactgta agttatcact gtactgatcg ctccagaacc acggcggcgg cagtcgcgga
    26941 agcggtagcc ccaacattgc ggcagcggca atttgcgcct ggttattggc gttttcccag
    27001 ctttcgcagc ggtgtagtgc accattatca atacgggtga ttgccacatc gccaccggca
    27061 aagatcgcgg gatcgcaggt gcggcaagct tcatcaatga caataccatt ggcagtatca
    27121 aggttggcct cgcgagccag ttggtcgttg gcgctgatac caataccgta aatcaccaca
    27181 tcggcccgaa gcgtttcgcc actttgcagc gtcagttcta ctttttcacc atcgaccaca
    27241 tgttcaatgg cattattgag cagaatgcgc acaccagcct gctgatggcg cggtaaaaga
    27301 tagcgttgca cgggcggtgg tgcattacgg cccatgacgc ttgccgccag ttcaatcact
    27361 gtcaccttac atccacgctg cgtggcgctg gcagccagtt ccagaccaat agttccggca
    27421 ccgacaatca cgactgaccg ttcgggctgc agaacttctc gcagtctggc ggcatcaccg
    27481 gcatggcgca gggtaaagca gcgttctccc agtgcatcaa gcaacggcag cggtcgagct
    27541 gccgcgccgg ttgctataaa aagctgatcc cagtgccagc tttcgccgtt agctaacact
    27601 aactcttgtg tgtcgcggcc caatgttttg atggttacac cggaatgcag atgaacattg
    27661 ttttcctgcc accagtgagc gggtaacaca gactgcaact gtggggaatc ttccagcaac
    27721 atggatttcg agagcggggg gcgttcataa ggaagatgtt gctcatcgga aaacagatgc
    27781 agctcaccgc tgaacccttg ctggcgtagc aaggccgcag ccattgccgc cgcttgcccg
    27841 ccaccgacaa taatgatcgt tttttctttc atcgtgcttc cttaaagatc cagcccagcc
    27901 gctacgtggc gaatgccgcg aatcgctaaa cccgcatcag cgttgatcat cacaccgctt
    27961 aatgcgcgat tattgcgccg cgatgccaac atcacatacg gacccgtaaa atccgccggt
    28021 tgcgggaaaa attgcagcgg taaaatggcg gcaattttct ccggcgtcag agactgcatt
    28081 atcgaggttt cactttgccc gagcgcctgt gggccgcgca ggtcgctggc cataccacac
    28141 gggccgacgc cattcacccg cactttcggt gccagttcat aagccagttg gcgaataagt
    28201 ccggttgcgg catgtttact ggcggtgtac agcgggccac cgccgccggg ataccaggcg
    28261 gcgtttgaca gtgtgaaaat catgctgcct tcactggcga ttaacgccgg agcgcaggct
    28321 tttgcgccca gcaggtaacc gagtacgttg acattaaaca gctcgtggaa gccggtttcg
    28381 agcgtctctg cgggagtgtt aaccagtgag gcattgtgat cccagatgcc cgcattgccg
    28441 ataaaacaat ccagcttgcc agaacgagtc aggatctgat taaccgcgcg ttgataatcg
    28501 gcatagcagg tcacgttacc ttccaccgcc agaatatgtt cgccaaatcg ctgacgcaga
    28561 ctggcgactt ttgccgccga cagttccagc gtggcaacct gcgcgccttc ttcgataaat
    28621 cgctcggcca gcgccaggcc taatcccgat ccgccgccgg taataaagat ggactcgtta
    28681 tgcagatcgc tcatggctgc gcctccggta agtcgatgaa gatgtcacca ccttcaacgt
    28741 gtactggata agtggttagc ggatcggtgg cgggcaggca taacgctttc cccgttttca
    28801 ggcaaaaact ggcggcgtgc agcgggcact ccaccgtggc gtcatcttcc agataccctt
    28861 ctgacatcga cgcattacca tggctgcaac gatcgttaat ggcataaaac tcgccgccta
    28921 cgttgaacag ggcgatgacg ggcgaggtat cgatccggag agcctcaccc tccggcacat
    28981 ccgcaacggg acacgcataa attcgattca tcagaacagt acactcaggt tatgggagga
    29041 gatgaccgct tgatccagga cgatatcccg ttccagcaag cgccagttcc cgtcctccag
    29101 acggcgaact ttgtcgaaac gcgttccaac atagaatgtt tcatcgcgct ctttttgtgc
    29161 ccgataaagt agataattca ccctcacggc aaatacgttt gggatgtcgg tttcgcttac
    29221 ctggcagttg ctgattaagt gacgagtgcg tgacggcggt tcttctgccc aggccatgcc
    29281 ggtttccaga cgggcgattc gccgttccag ctggtctttg gtgtcattaa aaatccaggt
    29341 tgtcggcggt tgaacgcctt tgcggcggtc gcgcgtttgt gcgttaaccg tggtgcgcat
    29401 ggtgtaacga atctcttcgt cgagctgtgc cagccagtca cgaaatttcc agtcatccag
    29461 cagactggct tcgtgaaaga gaaactggct aatgcggtga tgtaactcta gtgaaacttg
    29521 cgcactcatt tcatcacctc ctgctggtaa gcggcggttt tatcgagcac ttcctgccag
    29581 ctttcgctac tcagaagatc ggcccagcgt tggtacattc cacgagcggc cgtttctgag
    29641 aagatatagt tagtaatgcc tggaatgccg tcgtcgcgac gcttttcctg accaagcccc
    29701 atttccagac acagtttgct gttgcgggcg cggtggcctt tcagcaattt ctggatttca
    29761 caccagttct ccgagtcatc ctgctcgaga aaaccagcag gaccaaaagc acgagtggcg
    29821 ctgttttcaa aagcggcttt aacttcatcg gaggcggctt tgtcagtaat acagaacgcc
    29881 cacacttcga cttgatcagg gccgcgcgga tgccagacgc ggagcgtggc agtgccgttg
    29941 agccatgaaa gcgtggggaa aatattgtta tgacccgcca ggcgcagggc gcgaacttca
    30001 cctaaacgtt gttctgcttc ggcataggtt tcgcggtaat agctggaaac cgcgccatcg
    30061 acccagacat tagcatccgg tttttcagta aagaagaaac cgctaccgtg accgtcctga
    30121 ccaaattgca gcgcatcttt ggcggtttcc cacaccgggc gggcggtttg tccatcaccg
    30181 agacgcttat cgctgccatc atctttcgcc cctaatacct gaacggcaga agcatggctg
    30241 aacagagcat gatattggtc actggcgaac tgctctgccg ggaatttcca gttgcagttg
    30301 atcacccact tttgtacacc gccgacaatt tcggtgccgc cttcgcgacg atccagcatg
    30361 ccatccagat accaggcaat gtcacccagg taatcacgca ggcccggtgc gctggtatcc
    30421 cagttgccaa aaatcagccc tttataactc tccacacaag gaacttcgtt tagtccccag
    30481 tgggatttac acaacccttg tgggtaggcg cgaggttcca gcggtacatc gatcaactcg
    30541 ccgttaatgc cataagacca gccgtgatac ggacaggtaa aggcgcgagt gttaccgcaa
    30601 tctgcataac tcacacgcat ggcccggtgg cggcattggt tgagaaacgc cttaatgctg
    30661 ccgtctttct gacgcactac gacaaccgca tcttccccca tgtaggtgtt aaagaaatca
    30721 ccatgttttg ggatctggct ttcgtgggcg agaaatagcc agcaacgacc gaaaatgcgc
    30781 tcaagctcca gttggtaaat atccgggtcg gtataaatac gcggagtgac ccgaccgttt
    30841 tgggtgtcaa tcagttggta aatgttcaaa tctgagggtg tggtcatatt ttttaatcct
    30901 gaaagataag atgtattgac cttctatttt tgtgccataa ggatgttgct ggttggttgc
    30961 tgatatgtga aatagttaat gtctaccagc tgagacgttt tttcgataac aaggggaagg
    31021 tgatggaact acggcattta cgctatttcg tcgcagtggc gcaggcactg aactttaccc
    31081 gtgcggcgga aaaactgcat acctcacagc cttcgttaag cagccagatc cgcgatcttg
    31141 aaaactgtgt cggtgttccg ttgctggtga gggataagcg caaagtcgcg ttgacggcgg
    31201 cgggagaatg ttttctccag gacgcgctgg caatcctcga acaagcggaa aatgccaaat
    31261 tacgggcgcg gaaaattgtt caggaagaca gacaattaac cattggcttt gtgccatcgg
    31321 cggaagtgaa tttactgcca aaagtattac cgatgtttcg tctcagacag ccagacacct
    31381 taattgagct ggtgagttta atcaccacgg aacaggaaga aaaaattcgc cgtggtgaac
    31441 tcgacgtcgg cttaatgcgc catcctgttt atagcccgga gattgattat ctggagcttt
    31501 ttgacgaacc attagtggtg gtgttaccgg ttgatcaccc tttagcacat gaaaaagaga
    31561 tcactgccgc gcaactggat ggcgtgaatt tcgtcagtac tgatccagcg tattccggtt
    31621 cgcttgcgcc gatcgttaaa gcgtggtttg cgcaagaaaa cagccagcca aatatcgtcc
    31681 aggtggcaac gaatattctg gtgaccatga atctggtggg gatggggctg ggcgtcactt
    31741 tgatacccgg ttatatgaat aattttaata ccggccaggt ggtatttcga cccattgccg
    31801 gaaatgtgcc tgccattgct ttactgatgg cgtggaagaa aggggagatg aagccggcat
    31861 tacgcgattt catcgccatt gtgcaggaac gtttggcaag cgtaacggca taattacgca
    31921 ttatttttgc aaaacgcgtc gcggatcgca gttttttagt gcttgtgaac tggcgttaca
    31981 gacatcggga agcaggctcg aatattctta tttttcaact tgcaccaaca ttcgattaac
    32041 cccagactaa cgcctcctga cgggagggac tcatggtttt gcaatccacg cgctggttgg
    32101 cgctcggcta tttcacatac ttttttagtt acggcatttt tctacctttc tggagcgttt
    32161 ggcttaaggg gattggttta acgccagaaa ccatcggcct gttattgggg gcaggtctgg
    32221 ttgcccgttt tctcgggagc ttgctcatcg cgccccgcgt cagcgatcct tctcgcctga
    32281 tttccgcctt gcgcgtgctg gcactgctga cacttctctt tgctgtcgcc ttctgggcgg
    32341 gggcgcacgt agcgtggctg atgctggtga tgattggctt taacctcttt ttctcaccgc
    32401 tggtaccgtt gaccgatgca ctggcgaata cgtggcaaaa gcagttcccg cttgattacg
    32461 gcaaagtgcg attgtggggc tcggtggcgt ttgtcattgg ctcgtcgctg acgggcaaac
    32521 tggtcactat gtttgattat cgggtgatcc tcgcgctgtt gacgttgggc gtggcatcca
    32581 tgctgctcgg ctttctcatc cgtccgacga ttcagccaca aggggcaagc cgccagcagg
    32641 agagcaccgg ttggtctgcg tggttggcgt tggttcgcca gaactggcgc tttctggcct
    32701 gcgtttgttt attgcagggg gcgcatgcgg cctattacgg ttttagcgcc atttactggc
    32761 aggctgctgg ctactcggcc tcggcggtgg gctatttgtg gtcgctgggc gtggtggcgg
    32821 aagtcattat ctttgcgctg agtaataaac ttttccgccg ttgtagtgca cgcgatatgc
    32881 tgttgatctc agcgatttgc ggcgtagtgc gctggggcat tatgggagca actacggcgt
    32941 tgccgtggtt gatagtggtg caaattctgc attgtggcac cttcacggtc tgccacctgg
    33001 ccgccatgcg ctatattgcc gctcgccagg gtagcgaagt catccgttta caggcggttt
    33061 actctgccgt cgcgatgggc ggcagtattg cgatcatgac cgttttcgcc ggtttcctgt
    33121 atcaatatct gggccacggc gtgttctggg tgatggcgct ggtggcgctt ccggcaatgt
    33181 ttttgcgccc gaaagttgtt ccctcatgct gattcgagca ttttgcggat ctgctcctgt
    33241 tgatgtgttg tcaacgtcag gtccgtatag atcagcggtg gtgaaaagag cggtaacggc
    33301 atggtataag gagcaataat cagtgccgcg ctgcgcggag cgcctgtctg caaaaatgcc
    33361 tttaccgaca tatgtttaat attgagcggc agtaacgtta gttcgcgtag ctgctgctca
    33421 atctgcgctt ctcgctcgct atcattcccg gtcagtagaa taatctgttt ctcatgcagg
    33481 tcgttttcct gcattagcca ggcaccaaaa atcaccgcga ccagaccgct ttcctcatca
    33541 gaaagatgga cgccgtattc actttcaaat ccggccagcg ccgcgcgggt ggtgcgcacc
    33601 aggcgtggat acaggcgggc gaactcttcc ggcagggtgt tatcaatacc gatggcaaac
    33661 aaactgcgat ttaacgcctg ggcgaggtgg gtataaagct gatcgcataa cccctgttca
    33721 tcataaaaac gaacatttcc cagctcacga aaatggttta ccagacgttt gatagactta
    33781 cgcagttgtc tgtcccgctg atgcgcatcc cgcagtggat cgggaacccg caacatggaa
    33841 aaaagtaatg ccataaacag tggctcatca ggcggtacag gctggagcgc ccgacgctgc
    33901 cagtggcgtc caatttcctg cgctacctga aattcaaggc aggattccgc ccagcgacgt
    33961 tggagcggat taaactgagg cgtaattcca gcgtggtgtt gcagcaaaca atattgcaga
    34021 tacagacaca ggaaatgaat atcgcgcgtt tcaaagcgtt tttgcagccg tcgggagcag
    34081 agattcacta acgcttgtag attggtatcg tcgtaaaagt tacgcgcaat tccgcgccgc
    34141 ttcagttcac tcttcagggc gggggtgaaa tggctggtaa taaatgacgg acacagacgg
    34201 aaaccacgtc gtagccagtg aaataaacac aaacgttggt taagcactgt accttcaacc
    34261 cggtagctac cgtcataacc agtagtcagt gtgagttgat gatagcgcag gatctcccgt
    34321 cctgtttcgc tgatatcaag actggcaata tcatcatcca caccattaag ctcgctgaaa
    34381 gttgccgtgg tggcggtcaa ccccggctga aagagcgtca gcaagatctg gcagcggcgc
    34441 tggggagccg aaaggacaga tggtggagca agcgtaggca tcatctgtag atctcccata
    34501 agcataattg ataagaatag ctaaagtttg ctcagggaaa tggcgaaggg aatgctaatc
    34561 atcagaaatg ttggcaagcg tcacagaatt tttctcatct taaactgttc tgaccagtgg
    34621 attagcatgt cttaaggtgg ttttgtgaat gattatcgtg ctagcgatca tgggttagtt
    34681 ctgcggattc aacgcgctaa ggataaaatc caccatctgt tcgcgataaa catcgccgtg
    34741 acgatcggaa aaagcatcaa tgtgttcgcc atccgggatt aggatcaacc gttttggctc
    34801 ttttgccagg ctatacaact tttcgctgtg ctgccatggg ataacgtgat cagctttacc
    34861 gtgaatcagt aaaagcggga tcgggctgac gctggcgatg taattttcgc cgctgtaact
    34921 ctcatcaagt aagtagccac tgccggggat catctggttg gcgatggtcg cataagaggc
    34981 aaatgtggag tcgaggatca ccgcacgtat gccttcacga tcaccctgac caataacatc
    35041 caaaatattc gccccgccaa tactctgccc gaacagcacc agccgttgtg ggtttacatc
    35101 actgcgatgg cgcaccacat tgatggcact ttgcgtatcg tccagcaatc cggcctggga
    35161 cggcgtgcct tttgatttac caaacccgcg ataatcaaac ataaaaacgt tgaaattacg
    35221 ctcgggtaac caactgacca gcggccagtg ggcggacata tttccggcat tgccgtgagc
    35281 atgaataatg gtggcaatgg ccttttcagc ggggccagtt gcagaaggga taaaccaacc
    35341 atgtaggcga gtgccatctt tagcggtgaa ttctactgat tcggctgacc agggatcggg
    35401 gccgtagatt ttgtcgtcag ggtaatagaa aaaattgatg gcgatgcgcg gtattaaata
    35461 aatgcagaag gcaacaacaa agagaataaa cagaattttg agaacgcgtt tgttcactgg
    35521 cagcgccata acctttcctt gcgacttctt tatcatccat taatcgggga aagatggtgg
    35581 gagagtggcg aaagaaagta aagcggtgag gaagtatttc ggaatagcgc ctgaaaggcg
    35641 agggcgggtg agtgacatca cccgccagag tcattaacgc ttcagggcgt cgcttaactc
    35701 gtcacgcatg ttcgccagca tggctttaac aacgcgcggg ttaccagcaa cgatgttacc
    35761 ggtcagcatg tagttatgac caccggtgaa gtcgctgacg atgccgcccg cttcacgaac
    35821 cagcagctcg cctgccgcga aatcccacgg acgcagaccg atttcaaaga aaccgtcaac
    35881 gcgacccgca gcaacgtaag ccagatccag cgccgcagaa ccggtacgac ggaagtctgc
    35941 acattcgttg aacagtttgc cgacgatgtt gatgtaggta gtggcgtact gttttgcttt
    36001 gaacgggaag ccggtcgcca gaatagtacc gtcgagatcg cgagcggtgc tgccgcgcag
    36061 acggtagccg ttcagctgtg cgccctgacc gcgagtggcg gtgaacagtt cgttacgcat
    36121 aggatcgtaa accacagcaa cttcagtgcg gcctttgata cgaacggcga tagataccgc
    36181 gaagtgcggc agacgtttga taaagttggt agtgccatcc agtggatcga taacccattg
    36241 aacatcctga tcagtacctt caagttcacc gctttcttcg gtgatgatgg tgtgctgtgg
    36301 gtaagattta cgaatcgtgt cgataatcac cgcttcggca gctttatcta cgttggtcac
    36361 gaagtcgtta ctgcctttct ggctcgcttc tacagcgtcc ggggtttcat agtttttggc
    36421 aattaaatta cccgccttgc gcgctgcgcg cacggcgatg ttcagcatcg gatgcatcgg
    36481 tctctctcac tggatgttaa agaacgggaa aacggcgcag agtatagcag cgagatcggc
    36541 atctgtcttc gttttatgat aagatgcgtg aataatcttt agatgaagaa agacaatgct
    36601 gcaaaatatt cgaattgtgc tggtggagac gtcacacacc ggcaatatgg ggtctgttgc
    36661 ccgtgccatg aaaacaatgg gattaaccaa tctgtggctg gttaatccac tggtgaaacc
    36721 tgactcccag gcgattgccc tggcagcagg ggccagcgat gtgattggta atgctcacat
    36781 cgtcgatacg ctcgacgaag cgttagccgg ttgtagcctg gtggtgggca ccagcgcacg
    36841 ctcccgcacg ctgccgtggc cgatgctcga cccgcgcgaa tgcggtctga aaagcgtcgc
    36901 tgaagcggca aataccccgg tggcactggt gtttggtcgc gagcgcgtcg gcttgaccaa
    36961 tgaagagctg cagaaatgcc attatcatgt cgcgattgcg gctaacccgg aatacagttc
    37021 gctgaacctg gcgatggcgg ttcaggttat tgcctatgaa gtgcgcatgg cctggctggc
    37081 aactcaggaa aatggcgagc aggtcgagca tgaagagacg ccgtatccgc tggtcgatga
    37141 tctggagcgt ttttatggtc atctggaaca aacgctgctg gcaaccggtt ttattcgtga
    37201 aaaccatccg gggcaggtga tgaataaatt gcgccgtctg tttacccgtg cgcgcccgga
    37261 aagccaggaa ttgaatatcc tgcgcgggat tctggcttct atagagcagc agaataaagg
    37321 taacaaggcc gaataatagt ggttgcctga tgcgacgctt aacgcgtctt atcaggcctg
    37381 cagtgcacag agcccctggg cggataaggc gttcacgtcg catccgacaa caggtacaaa
    37441 cgccacgata aaaaaatggc actgaaggtt aaatacccga ctaaatcagt caagtaaata
    37501 gttgaccaat ttactcggga atgtcagact tgaccctgct atgcaatacc cccactttta
    37561 caataaaaaa ccccgggcag gggcgagttt gaggtgaagt aagacatgag actgacatct
    37621 aaagggcgct atgccgtgac cgcaatgctt gacgttgcgc tcaactctga agcgggcccg
    37681 gtaccgttgg ctgatatttc cgaacgtcag ggaatttccc tttcttatct ggaacaactg
    37741 ttttcccgtc tgcgtaaaaa tggtctggtt tccagcgtac gtggaccagg cggtggttat
    37801 ctgttaggca aagatgccag cagcatcgcc gttggcgaag tgattagcgc cgttgacgaa
    37861 tctgtagatg ccacccgttg tcagggtaaa ggcggctgcc agggcggcga taaatgcctg
    37921 acccacgcgc tgtggcgtga tttgagcgac cgtcttaccg gttttctcaa taacattact
    37981 ttaggcgaac tggttaataa ccaggaagtg ctggatgtgt ctggtcgtca gcatactcac
    38041 gacgcgccac gcacccgcac acaagacgcg atcgacgtta agttacgcgc ttaataaaaa
    38101 gaattcagaa tcaggccgga gtgctaaaca ctccgtaaac acggtcgtac atccagccgg
    38161 tagcctggtt ccttgcattg agtgatgtac ggagtttata gagcaatgaa attaccgatt
    38221 tatctcgact actccgcaac cacgccggtg gacccgcgtg ttgccgagaa aatgatgcag
    38281 tttatgacga tggacggaac ctttggtaac ccggcctccc gttctcaccg tttcggctgg
    38341 caggctgaag aagcggttga tatcgcccgt aatcagattg ccgatctggt cggcgctgac
    38401 ccgcgtgaaa tcgtctttac ctctggtgca accgaatctg acaacctggc gatcaaaggt
    38461 gcagccaact tttatcagaa aaaaggcaag cacatcatca ccagtaaaac cgaacataaa
    38521 gcggtactgg atacctgccg tcagctggag cgcgaaggtt ttgaagtcac ttacctggca
    38581 ccgcagcgta atggcattat cgacctgaaa gaactcgaag cagcgatgcg tgacgacacc
    38641 atccttgttt ccatcatgca cgtaaataac gaaatcggcg tggtgcagga tatcgcggct
    38701 atcggcgaaa tgtgccgtgc tcgtggcatt atctatcacg ttgatgcaac ccagagcgtg
    38761 ggcaaactgc ctatcgatct gagccagttg aaagttgacc tgatgtcttt ctccggtcac
    38821 aaaatctatg gcccgaaagg tattggtgcg ctgtatgtgc gtcgtaaacc gcgcgtacgt
    38881 atcgaagcac aaatgcatgg cggcggtcac gagcgcggta tgcgttccgg cactctgcct
    38941 gttcaccaga tcgtcggtat gggcgaagcc tatcgcatcg caaaagaaga gatggcgacc
    39001 gagatggaac gtctgcgcgg cctgcgtaac cgtctgtgga acggcatcaa agatatcgaa
    39061 gaagtttacc tgaacggtga cctggaacac ggtgcgccga acattctcaa cgtcagcttc
    39121 aactacgttg aaggtgagtc gctgattatg gcgctgaaag acctcgcggt ttcttcaggt
    39181 tccgcctgta cgtcagcaag cctcgaaccg tcctacgtgc tgcgcgcgct ggggctgaac
    39241 gacgagctgg cacatagctc tatccgtttc tctttaggtc gttttactac tgaagaagag
    39301 atcgactaca ccatcgagtt agttcgtaaa tccatcggtc gtctgcgtga cctttctccg
    39361 ctgtgggaaa tgtacaagca gggcgtggat ctgaacagca tcgaatgggc tcatcattaa
    39421 tcggtatcgg aatcaggaga atttataatg gcttacagcg aaaaagttat cgaccattac
    39481 gagaatccgc gtaacgtggg ttcctttgac aacaacgacg agaacgtcgg cagcggcatg
    39541 gtgggtgcac cggcctgtgg cgacgtgatg aagttgcaga ttaaagtcaa cgatgaaggt
    39601 atcattgaag acgcgcgttt taaaacttac ggctgcggtt ctgctatcgc ttccagctcc
    39661 ctggtcaccg aatgggtgaa agggaagtct ctcgacgaag cgcaggcgat caaaaacacc
    39721 gatattgctg aagaacttga actgccgccg gtgaaaattc actgttctat tctggcagaa
    39781 gacgcgatca aagccgccat tgcggactat aaaagcaaac gtgaagcaaa ataagagttg
    39841 aggtttggtt atgtcgatta cactgagcga cagtgcagca gcgcgagtaa atacctttct
    39901 ggctaaccgc ggcaaagggt ttggcctgcg tctgggcgtg agaacctccg gatgttcagg
    39961 tatggcttat gtactggaat ttgttgacga accgacgccg gaagacatcg tgtttgaaga
    40021 caaaggcgtg aaagtcgtgg tcgatggcaa aagcctgcaa tttctggacg gtacgcagct
    40081 ggacttcgta aaagaaggcc tgaacgaagg gtttaaattc accaacccga acgtcaaaga
    40141 tgagtgtggt tgcggcgaaa gcttccacgt ttgatgcgca taccgataac cccaccgtgg
    40201 tcgcctgcgc gtggggtttg ttttacctga ttcgccgtta tcgcggcgga tcgcagccct
    40261 gagaatgtta tggattactt caccctcttt ggcttgcctg cctgttatca actcgatacc
    40321 caggcgctga gcctgcgttt tcaggatcta caacgtcagt atcatcctga taaattcgcc
    40381 agcggaagcc aggcggaaca actcgccgcc gtacagcaat ctgcaaccat taaccaggcc
    40441 tggcaaacgc tgcgtcatcc gttaatgcgc gcggaatatt tgctttcttt gcacggtttt
    40501 gatcttgcca gcgagcagca tactgtgcgc gacaccgcgt tcctgatgga acagctggaa
    40561 ctgcgcgaag aactggacga gatcgaacag gcgaaagatg aagcgcggct ggaaagcttt
    40621 atcaaacgtg tgaaaaagat gtttgatacc cgccatcagt tgatggttga acagttagac
    40681 aacgagacgt gggacgcggc ggcggatacc gtgcgtaagc tgcgttttct cgataaactg
    40741 cgaagcagtg ccgaacaact cgaagaaaaa ctgctcgatt tttaatttct ggaagctaaa
    40801 catggcctta ttacaaatta gtgaacctgg tttgagtgct gcgccgcatc agcgtcgtct
    40861 ggcggccggt atcgaccttg gcacaaccaa ctcgctggtg gcgacagtgc gcagcggtca
    40921 ggccgaaacg ttagccgacc atgaaggccg tcacctgctg ccgtctgttg ttcactatca
    40981 acagcaaggg cattcggtgg gttatgatgc gcgtactaac gcagcgctcg ataccgccaa
    41041 caccattagt tctgttaaac gcctgatggg gcgctcgctg gctgatatcc agcaacgcta
    41101 tccacatctg ccttatcaat tccaggccag cgaaaacggc ctgccgatga ttgaaacggc
    41161 ggcggggctg ctgaacccgg tgcgcgtttc tgcggacatc ctcaaagcac tggcggcgcg
    41221 ggcaactgaa gccctggcag gcgagctgga tggtgtagtt atcaccgttc cggcgtactt
    41281 tgacgatgcc cagcgtcagg gcaccaaaga cgcggcgcgt ctggcggggt tgcatgttct
    41341 gcgcttactt aacgaaccga ccgctgcggc tatcgcctac gggctggatt ccggtcagga
    41401 aggcgtgatc gccgtttatg acctcggcgg cgggacgttt gatatttcca ttctgcgctt
    41461 aagtcgcggc gtgtttgaag tgctggcgac cggcggtgat tccgcgctcg gcggcgatga
    41521 tttcgaccat ctgctggcgg attacattcg cgagcaggcg ggcattcctg atcgtagcga
    41581 taaccgcgtt cagcgtgaac tgctggatgc cgccattgca gccaaaatcg cgctgagcga
    41641 tgcggactcc gtgactgtga atgttgcggg ctggcagggc gaaatcagcc gtgaacaatt
    41701 caacgaactg atcgcgccac tggtaaaacg aaccttgctg gcttgtcgtc gcgcgctgaa
    41761 agacgctggc gtagaagctg atgaagtgct ggaagtggtg atggttggcg gttctactcg
    41821 cgtgccgcta gtgcgtgaac gagtaggcga atttttcggt cgtccaccgc tgacttctat
    41881 cgacccggat aaagtcgtcg ctattggcgc ggcgattcag gcggatattc tggtgggtaa
    41941 caagccggac agcgaaatgc tgttgctgga tgtgatcccg ctgtcgctgg gcctcgaaac
    42001 gatgggcggc ctggtggaga aagtgattcc gcgtaatacc actattccgg tggcgcgcgc
    42061 tcaggatttc accaccttta aagatggtca gacggcgatg tctatccatg taatgcaggg
    42121 tgagcgcgaa ctggtgcagg actgccgctc gctggcgcgt tttgcgctgc gtggtattcc
    42181 ggcgttaccg gctggcggcg cgcatattcg cgtgacgttc caggtcgatg ccgacggtct
    42241 tttgagcgtg acggcgatgg agaaatccac cggcgttgag gcatctattc aggtcaaacc
    42301 gtcctacggt ctgaccgaca gcgaaatcgc ctcgatgatc aaagactcaa tgagctatgc
    42361 cgagcaggac gtaaaagccc gtatgctggc agaacaaaaa gtagaagcgg cgcgtgtgct
    42421 ggaaagtttg cacggcgcgc tggctgctga tgccgcgctg ttaagcaccg cagagcgtca
    42481 ggtcattgac gatgctgccg ctcacctgag tgaagtggcg cagggcgatg atgttgacgc
    42541 catcgaacaa gcgattaaaa acgtagacaa acaaacccag gatttcgccg ctcgccgcat
    42601 ggaccagtcg gttcgtcgtg cgctgaaagg ccattccgtg gacgaggttt aatatgccaa
    42661 agattgttat tttgcctcat caggatctct gccctgatgg cgctgttctg gaagcgaata
    42721 gtggtgaaac cattctcgac gttgcgctgc gtaacggtat cgagattgaa cacgcctgtg
    42781 aaaaatcctg tgcttgcacc acctgccact gcatcgttcg cgaaggtttt gactcgctgc
    42841 cggaaagctc agagcaggaa gacgacatgc tggacaaagc ctggggactg gagccggaaa
    42901 gccgtttaag ctgccaggcg cgcgttaccg acgaagattt agtggtcgaa atcccgcgtt
    42961 acactatcaa ccatgcgcgt gagcattaac agaggttagt atgggactta agtggaccga
    43021 tagccgcgaa attggcgaag cactgtacga tgcgtatccc gatcttgatc cgaaaacggt
    43081 tcgattcacc gatatgcatc agtggatttg cgatctggaa gatttcgacg acgacccgca
    43141 ggcatcgaac gagaaaatcc tcgaagcgat tttgttggtc tggctggacg aagccgagtg
    43201 aatgaacggg ctgcctgggg gtagcccgtt ttgctgttaa acgaataagg ataactaaaa
    43261 tgacagaagc gatgaaaatt accctttcta cccaacctgc cgacgcgcgc tggggagaaa
    43321 aagcgactta cagcattaat aatgatggca ttaccctgca tttgaacggg acagacgatc
    43381 tggggctgat ccagcgtgcg gcgcgcaaga ttgacggtct gggcatcaag catgttcagt
    43441 taagcggtga agactgggat gcggatcgct gctgggcatt ctggcaaggt tacaaagccc
    43501 cgaaaggcac acgtaaagtg gagtggccgg atctggacga tgcccaacgg caggaactgg
    43561 ataaccgcct gatgatcatc gactgggtgc gtgacaccat caacgcaccg gcggaagaac
    43621 tggggccatc gcaactggca cagcgtgctg ttgatctgat cagcaacgtc gcgggcgatc
    43681 gtgtgactta ccggatcacc aaaggcgaag atctgcgtga tcaaggttat atggggctgc
    43741 acacggtcgg gcgcggttca gaacgttccc cggtattgct ggcgctggat tacaacccaa
    43801 caggcgataa agaagcgcca gtgtacgcgt gcctggttgg taaaggcatc acctttgatt
    43861 ccggcggcta cagtatcaaa cagaccgcat ttatggactc gatgaagtcg gacatgggcg
    43921 gcgcggcaac ggttaccgga gcgctggcgt ttgccattac gcgcggactg aacaagcgcg
    43981 tgaagctgtt cctctgctgt gcggataacc tgatcagcgg caatgcgttc aagctgggcg
    44041 atatcattac ctatcgcaac ggtaaaaaag ttgaagtgat gaacaccgat gcggaagggc
    44101 gtctggtgct ggccgatggc ctgattgatg ccagtgcgca gaaaccggaa ctgatcattg
    44161 atgcggcgac cctcaccggg gcggcgaaaa ccgcgctggg taatgattat cacgcgctgt
    44221 tcagttttga cgatgcgctt gccgctcgct tgctggcgag tgccgcgcag gagaatgaac
    44281 cattctggcg tctgccgctg gcggagttcc accgcagcca gctgccgtct aactttgccg
    44341 aactgaacaa taccggaagc gcggcgtatc cggcaggtgc aagcacggcg gcgggcttcc
    44401 tgtcgcactt tgttgagaac tatcagcaag gctggctgca tatcgactgc tcggcgactt
    44461 accgtaaagc gccggttgaa cagtggtctg cgggcgcgac cgggctaggt gtgcgcacga
    44521 ttgctaatct gttaacggcg taaacgtctt atttggccta caaaattgcg aaaatccaat
    44581 agattgagat tcccttgtag gcctgataag cgtagcgcat caggcacttg tgccccgaac
    44641 gttgaatgaa ttgtggttct tatgtccgaa acaaaaaacg aacttgaaga cctgctggaa
    44701 aaagcagcaa ccgaaccggc gcaccgcccg gcctttttcc gtactctact ggaatccacc
    44761 gtctgggtgc cgggtacggc ggcgcagggc gaggctgtgg ttgaagatag cgcgcttgat
    44821 ttacagcact gggaaaaaga agacggcacc agcgtcattc ctttttttac ctcgttagaa
    44881 gcacttcaac aggcggttga agacgaacag gcatttgtcg taatgcccgt tcgcacattg
    44941 tttgagatga cccttggcga aacgctcttc cttaatgcca aactgccaac cggtaaagaa
    45001 tttatgccgc gtgaaatcag tttgctgatt ggtgaagaag gaaatccgct gagcagccag
    45061 gaagtactgg aaggcggtga atcgctgata ttatcggaag tcgcagagcc accagcacaa
    45121 atgattgatt cactcaccac cttatttaaa actattaagc cggtgaagcg tgcttttatt
    45181 tgttcaatta aagagaacga agaggcacag cctaatttac ttattggcat tgaagccgat
    45241 ggtgatatcg aagaaattat tcaggcggcg ggaagtgtag cgaccgatac attacctggc
    45301 gatgaaccca tcgatatttg tcaggtgaaa aaaggggaaa aaggaattag ccactttatt
    45361 accgaacata ttgcgccatt ctatgaacgt cgctggggtg gttttttgcg tgactttaaa
    45421 cagaaccgga taatctaaaa tatgccgccc caaagggcgg cattcgggct tacttgctgc
    45481 tctgaaggtt gttaaccgcc agtaaattgt tgccggagag actccggtta ttatttacct
    45541 cgcttaacag cgcaccagaa acaataagta cgatacaaaa gcaccgtatc ttcattgtgc
    45601 ggtccatgcg aagaccgcat cacgacagca ccactggcga tattgccgcg atagcaagcg
    45661 ctgagacgcg cttgctctaa cggtcgcgga acatcctttc cttaagccag cgcccgcttg
    45721 cgagacggcg gaaaggtccg cagtggataa tacactaaca gaacaataat aactctcttt
    45781 tacttgacaa aaagagagtt actggcgagt attgtgtccg ctgagacgcg cttgcattac
    45841 tacggtaata caatctgctg gaaaggccgc gaaccaaacc agcaacaaat aaccgcctga
    45901 ttttggcggt tttttgtttt ttaaaggaaa gtgaatttac atg
//
LOCUS       DAZKGF010000016        45730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZKGF010000016 DAZKGF010000000
VERSION     DAZKGF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.016"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(51..203)
                     /gene="hokA"
                     /locus_tag="ACHOQV_000887"
     CDS             complement(51..203)
                     /gene="hokA"
                     /locus_tag="ACHOQV_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFP9629301.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMVRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(525..2594)
                     /gene="glyS"
                     /locus_tag="ACHOQV_000888"
     CDS             complement(525..2594)
                     /gene="glyS"
                     /locus_tag="ACHOQV_000888"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP9629302.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNTNVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAASLAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2604..3515)
                     /gene="glyQ"
                     /locus_tag="ACHOQV_000889"
     CDS             complement(2604..3515)
                     /gene="glyQ"
                     /locus_tag="ACHOQV_000889"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP9629303.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3610..3918)
                     /locus_tag="ACHOQV_000890"
     CDS             complement(3610..3918)
                     /locus_tag="ACHOQV_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFP9629304.1"
                     /translation="MMMNSFFPAMALMVLMALVGCSTPPPVQKAQRVKVDPLRSLNME
                     ALCKDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR
                     "
     gene            4092..5087
                     /gene="wecH"
                     /locus_tag="ACHOQV_000891"
     CDS             4092..5087
                     /gene="wecH"
                     /locus_tag="ACHOQV_000891"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFP9629305.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLLFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVCAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAITLLTLVKNTLDTRTVRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(5129..5566)
                     /gene="yiaA"
                     /locus_tag="ACHOQV_000892"
     CDS             complement(5129..5566)
                     /gene="yiaA"
                     /locus_tag="ACHOQV_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFP9629306.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5611..5952)
                     /gene="yiaB"
                     /locus_tag="ACHOQV_000893"
     CDS             complement(5611..5952)
                     /gene="yiaB"
                     /locus_tag="ACHOQV_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFP9629307.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGILMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            complement(6121..7575)
                     /gene="xylB"
                     /locus_tag="ACHOQV_000894"
     CDS             complement(6121..7575)
                     /gene="xylB"
                     /locus_tag="ACHOQV_000894"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP9629308.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWSMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7647..8969)
                     /gene="xylA"
                     /locus_tag="ACHOQV_000895"
     CDS             complement(7647..8969)
                     /gene="xylA"
                     /locus_tag="ACHOQV_000895"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFP9629309.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            9335..10327
                     /gene="xylF"
                     /locus_tag="ACHOQV_000896"
     CDS             9335..10327
                     /gene="xylF"
                     /locus_tag="ACHOQV_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9629310.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            10405..11946
                     /gene="xylG"
                     /locus_tag="ACHOQV_000897"
     CDS             10405..11946
                     /gene="xylG"
                     /locus_tag="ACHOQV_000897"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFP9629311.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11924..13105
                     /gene="xylH"
                     /locus_tag="ACHOQV_000898"
     CDS             11924..13105
                     /gene="xylH"
                     /locus_tag="ACHOQV_000898"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFP9629312.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            13183..14361
                     /gene="xylR"
                     /locus_tag="ACHOQV_000899"
     CDS             13183..14361
                     /gene="xylR"
                     /locus_tag="ACHOQV_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFP9629313.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14469..15293)
                     /locus_tag="ACHOQV_000900"
     CDS             complement(14469..15293)
                     /locus_tag="ACHOQV_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFP9629314.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15613..17640
                     /gene="malS"
                     /locus_tag="ACHOQV_000901"
     CDS             15613..17640
                     /gene="malS"
                     /locus_tag="ACHOQV_000901"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP9629315.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPTFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALVEWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAA
                     SVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17818..19071
                     /gene="avtA"
                     /locus_tag="ACHOQV_000902"
     CDS             17818..19071
                     /gene="avtA"
                     /locus_tag="ACHOQV_000902"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFP9629316.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRIKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMSEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(19223..19696)
                     /locus_tag="ACHOQV_000903"
     CDS             complement(19223..19696)
                     /locus_tag="ACHOQV_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9629317.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19798..20646)
                     /gene="yiaJ"
                     /locus_tag="ACHOQV_000904"
     CDS             complement(19798..20646)
                     /gene="yiaJ"
                     /locus_tag="ACHOQV_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFP9629318.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            20847..21845
                     /gene="yiaK"
                     /locus_tag="ACHOQV_000905"
     CDS             20847..21845
                     /gene="yiaK"
                     /locus_tag="ACHOQV_000905"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFP9629319.1"
                     /translation="MKVTFEQLKAAFNRVLISRDVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21857..22324
                     /locus_tag="ACHOQV_000906"
     CDS             21857..22324
                     /locus_tag="ACHOQV_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFP9629320.1"
                     /translation="MIFGHIAQPDPCRLPAAIEKALDFLRTTDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAAENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTTSEIRKIVVKVALTALN"
     gene            22442..22915
                     /gene="yiaM"
                     /locus_tag="ACHOQV_000907"
     CDS             22442..22915
                     /gene="yiaM"
                     /locus_tag="ACHOQV_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFP9629321.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22918..24195
                     /gene="yiaN"
                     /locus_tag="ACHOQV_000908"
     CDS             22918..24195
                     /gene="yiaN"
                     /locus_tag="ACHOQV_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFP9629322.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            24208..25194
                     /gene="yiaO"
                     /locus_tag="ACHOQV_000909"
     CDS             24208..25194
                     /gene="yiaO"
                     /locus_tag="ACHOQV_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFP9629323.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGAELLKAVDEVQ"
     gene            25198..26694
                     /gene="lyxK"
                     /locus_tag="ACHOQV_000910"
     CDS             25198..26694
                     /gene="lyxK"
                     /locus_tag="ACHOQV_000910"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFP9629324.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPLVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTTQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRVHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            26691..27353
                     /gene="ulaD"
                     /locus_tag="ACHOQV_000911"
     CDS             26691..27353
                     /gene="ulaD"
                     /locus_tag="ACHOQV_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9629325.1"
                     /translation="MSRPLLQLALDHSSLEDAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPNKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPVQVAGDFHAQIDAIWGGA
                     RA"
     gene            27346..28206
                     /locus_tag="ACHOQV_000912"
     CDS             27346..28206
                     /locus_tag="ACHOQV_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP9629326.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWTVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            28200..28895
                     /gene="araD"
                     /locus_tag="ACHOQV_000913"
     CDS             28200..28895
                     /gene="araD"
                     /locus_tag="ACHOQV_000913"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9629327.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDEARQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(28927..29742)
                     /locus_tag="ACHOQV_000914"
     CDS             complement(28927..29742)
                     /locus_tag="ACHOQV_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9629328.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADAAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            29968..31368
                     /locus_tag="ACHOQV_000915"
     CDS             29968..31368
                     /locus_tag="ACHOQV_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9629329.1"
                     /translation="MTSTPITAADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILCNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTTSSLGGITAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            31379..33358
                     /locus_tag="ACHOQV_000916"
     CDS             31379..33358
                     /locus_tag="ACHOQV_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFP9629330.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQDGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNAYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPGESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHTVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKT
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGKGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHCHPEVG"
     gene            complement(33479..34219)
                     /locus_tag="ACHOQV_000917"
     CDS             complement(33479..34219)
                     /locus_tag="ACHOQV_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFP9629331.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPTDNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            34343..35317
                     /locus_tag="ACHOQV_000918"
     CDS             34343..35317
                     /locus_tag="ACHOQV_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9629332.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTVLLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAQPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(35314..36450)
                     /locus_tag="ACHOQV_000919"
     CDS             complement(35314..36450)
                     /locus_tag="ACHOQV_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9629333.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSAAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(36456..36779)
                     /locus_tag="ACHOQV_000920"
     CDS             complement(36456..36779)
                     /locus_tag="ACHOQV_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP9629334.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDVPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            36962..37105
                     /locus_tag="ACHOQV_000921"
     CDS             36962..37105
                     /locus_tag="ACHOQV_000921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629335.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGTPMVIYGLVRPLTIE
                     TRD"
     gene            complement(37324..38862)
                     /gene="aldB"
                     /locus_tag="ACHOQV_000922"
     CDS             complement(37324..38862)
                     /gene="aldB"
                     /locus_tag="ACHOQV_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFP9629336.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(38970..40265)
                     /locus_tag="ACHOQV_000923"
     CDS             complement(38970..40265)
                     /locus_tag="ACHOQV_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFP9629337.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKHATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            complement(40395..41546)
                     /gene="yiaY"
                     /locus_tag="ACHOQV_000924"
     CDS             complement(40395..41546)
                     /gene="yiaY"
                     /locus_tag="ACHOQV_000924"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFP9629338.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAEGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMVGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     NIPAGLRDLNVKEEDIPVLATNALKDACGFTNPIQATHEEIMAIYRAAM"
     gene            complement(41737..43581)
                     /gene="selB"
                     /locus_tag="ACHOQV_000925"
     CDS             complement(41737..43581)
                     /gene="selB"
                     /locus_tag="ACHOQV_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFP9629339.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(43578..44969)
                     /gene="selA"
                     /locus_tag="ACHOQV_000926"
     CDS             complement(43578..44969)
                     /gene="selA"
                     /locus_tag="ACHOQV_000926"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFP9629340.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(45067..45675)
                     /locus_tag="ACHOQV_000927"
     CDS             complement(45067..45675)
                     /locus_tag="ACHOQV_000927"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFP9629341.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
BASE COUNT        11183 a        11949 c        11385 g        11213 t
ORIGIN      
        1 gaatgcgcaa cgccgtaaga aaggcgggaa taacttcccg ccaaagtctc ttactctttc
       61 aaattgcagg ctaaaaatgc cgccagctca taacttccct gtttaatatg caattcacac
      121 agtgaatctc ttaccatcca ggtaaaaaat aaaagcgtga aacaaatcac tattaaagaa
      181 agcaatctat atttctgcgg cattcccagc ctcctgtgtt gatttccaac gagtaagagg
      241 ctaaccttat ggtgttcagg catagggtag cctcactttg atttatagtc aggtggggct
      301 tttctctgtc tgcctttcgg tgaatacctg agacaaacag tctcaagcac ccacagctat
      361 tctagcttaa taagttcgtt tcttctcctt gatataatcc taaaaaaatc tcataaaatt
      421 aatatatgag acaatcttta ttcagcagaa gattattaaa ggttgctcta ttatttagcg
      481 ataaaaaagc ctgccagatg gcaggctttt taataacggc gttattactg taacagcgaa
      541 atatccgcaa cgcgcaggaa taattcgcgc agtttctcca gcatggtaag acggttgatt
      601 cgcaattctt tgtcatcaac catgaccatc actttatcga agaaggcatc aaccggttca
      661 cgcaattcag ccagttcgac cagcgcgtcc tggtaacgac cttcagcgaa gtacggctcc
      721 agcttgtcgc gcagcaccac aacttgcatc gccagtttaa tttcttccgg ctctttcaga
      781 gtagaagcat tcacgcgatc gctcagcact tcgtcagact tcgccagaat gttagataca
      841 cgcttgtttg ccgccgccag tgacgctgcc gcatccagag tacggaagtg cgataccgcc
      901 ttcatacggg catcgaaatc tgccggacga gtcggacgac gcgccagtac cgcctggatg
      961 gtatcaacgg tgtaaccttc gtcctgatac caggcgcgga agcgaccgag cataaagtcg
     1021 ataacatcat caactacgtt ggtattagtc agcttatcgc catacagacg caccgcttct
     1081 tcggtcagcg tttgcagatc aaggttgagg ttcttctcaa cgataatacg cagcacgcca
     1141 agtgcggcac gacgcaacgc aaacgggtct ttgtcgcctt tcggatgctg gccgataccg
     1201 aagatacccg ccagagtatc catcttgtca gcaatcgcca gcgcacaggc taccgggtta
     1261 gacggcaggt catcaccggc aaagcgcggc tgatactgct cgttcagcgc cacggcaaca
     1321 tcttccgctt cgccatcgtg acgcgcgtag tgcatcccca taacgccctg ggtgtcggtg
     1381 aactcgaaga ccatgttggt catcaggtcg cacttggaca gcaggcccgc acgggtagcg
     1441 tggttaacgt cagcgccaat ctgttcagca atccagccag ccagcgcctg gatgcggtca
     1501 gtcttgtcac gcagcgtacc cagctgttgc tggaacagca cggtttgcag gcgcggcagg
     1561 ttatcttcca gacgtttttt acggtcggtg ttgaagaaga actcggcatc cgccagacgt
     1621 ggacgaacga ctttctcgtt accagagata atttgctgcg gatctttcga ttcgatgttg
     1681 gcaacgaaga taaagttcgg cagcagtttg ccgtcgttcg catacaccgg gaagtatttc
     1741 tggtcacctt tcatggtgta aaccagcgct tcagacggca ccgcgaggaa tttctcttcg
     1801 aattttgcgg tcagcacaac cggccattcc accagcgaag cgacttcttc caacaggctt
     1861 tcgcttaagt cagcgttacc gccaatctta cgcgccgctt cttcggcatc ggctttaatc
     1921 ttcgccttac gttcttcgta atcggcgatg actttcccgc gctcacgcag aatttccgga
     1981 tactgatcag cattgtcgat agtgaattcc ggctcgccca taaagcggtg gccgcgaatc
     2041 acgcgatcgg actgaatgcc cagaatggta gccggaataa ctttgtcgcc cagcagcagg
     2101 gtcacggtgt gcaccggacg cacgaagtgc acgtcgcttg caccccaacg catcagtttc
     2161 gggatcggca gtttcgccag cgaagtcgca accatattcg gcagcagtgc ttcagtgctt
     2221 tcgcccttca cgtgggcgcg atacagcagc cattcgcctt tatcggtagt cagacgctca
     2281 gcctggtcaa cggtaatacc gcaaccacgc gcccagcctt ctgccgcttt gctcggtttg
     2341 ccttcagcgt cgaacgcctg ggcaatcgcc gggccgcgtt tttcgatttc acgatccggt
     2401 tgcgcttccg ccaggttagc tactttaagc gccagacgac gcggagcagc aaaccattga
     2461 acggtgccgt gtgcgaggcc agcgttatcc agctccgcag taaagttcgc agcaaaggac
     2521 tcagccaggc tgcgcagtgc ttttggtggc agctcttcag tgccgatttc caccagaaaa
     2581 gttttctcag acatagccgc ctcttactta tctttgttgc acatcgggaa gccgagggct
     2641 tcacgggaag cgtagtatgc ttctgccact gctttggtca gggtgcgaat gcgcagaata
     2701 tagcgctgac gctcggtgac ggagatggct ttacgcgcat ccagcaggtt gaagctgtgg
     2761 gcggctttca gaatacgctc gtaggctggc agcggcagcg gattttccag cgccagcagc
     2821 tgctgcgctt ctttctcgta ctgctcaaag caggtgaaca ggaagtccac atccgcgtat
     2881 tcgaagttgt aagtggactg ctccacttcg ttctgatgga acacgtcgcc ataggtggtt
     2941 ttacccagcg ggccgtcgct ccagaccagg tcgtaaacgc tgtctacgcc ctgaatgtac
     3001 attgccagac gttccagacc gtaggtgatc tcgccggtaa ccggtttaca ctccagacca
     3061 ccaacctgct ggaagtaagt gaactgcgtc acttccatgc cgttcagcca cacttcccag
     3121 cccagccccc aggcacccag cgtcgggttt tcccagttat cttccacgaa acggatgtcg
     3181 tggatggtcg ggtccatgcc cagctctttc agagaaccaa ggtacagctc ctgaatattg
     3241 tccggcgatg gcttaatgac cacctggaac tgatagtagt gctgtaaacg gttggggttt
     3301 tcgccgtagc gaccatcggt cgggcgacga gaaggctgaa cataagcagc cgccatcggt
     3361 tctggcccca gcgcgcgcag acaggtcatt gggtgagagg ttcccgcgcc gacttccatg
     3421 tccaatggtt gaacaatggt gcagccctgg cgagcccagt aatcctgtaa ggtcaggatc
     3481 aagccctgga aggtcctggt atcaaacttt tgcatattat ttcgtgctgg atacgtgtgg
     3541 atttaaagga agggatcagt atacccgctg gatggaagat atacagtacg aaacgggaaa
     3601 aagcagggct taacgcatgg aaagatgcaa aaaatggcca tctgcgtcaa aagaacagac
     3661 aaactgctct ttacggaagg tataaccgcg catttcgtag cttccctgga actgttcaaa
     3721 tgcggtgacg tcgatttttt gctcgccggt gttataacgt ttcgccgcct gatccttgca
     3781 taacgcttcc atattcaacg aacgcagagg atcaaccttt acccgttgtg ctttctgcac
     3841 gggtggcggt gtagaacaac ccactagcgc cataagcacc ataagcgcca ttgccggaaa
     3901 gaaagagttc atcatcatta aattaccgtc tggtttgcag gtgagtctta ttatttatat
     3961 cggtatagtg gctgatatct tacgaatttc cttaagcctc tataagctgt agtgataaaa
     4021 ctcagattat tccagtgcgc caaccgaatg atttatcggt cagacttttt taaccagata
     4081 cagagacaca gatgcagccc aaaatttact ggattgataa cctgcgaggg atagcgtgtt
     4141 taatggtggt gatgattcac accactacct ggtatgtgac caatgctcat agtgttagcc
     4201 ccgtcacctg ggatatcgcc aatgttctga attctgcctc tcgtgtcagc gtgccgctat
     4261 ttttcatgat ttccggctat ctcttttttg gcgaacgcag cgcccagccg cgccatttct
     4321 tgcgtatcgg cttatgtctg cttttttata gcgcaatcgc actgctctac attgcgctgt
     4381 ttacctccat caatgtggag ttagcgctga aaaacctgct gcaaaagcca gtgttttacc
     4441 acttatggtt tttcttcgcg attgcggtga tttatctggt ttcaccgctg attcaggtga
     4501 agaacgtcgg cggaaaaatg ttgctggtgc taatggtggt gattggtatt atcgctaacc
     4561 caaacacagt gccgcagaaa attgacggtt ttgaatggct gccaattaac ttatatatca
     4621 atggcgatac tttttactac atcctgtatg gcatgttggg ccgcgctata gggatgatgg
     4681 acacacagca taaagcactg tcgtgggtgt gcgcagcact gtttgcgacg ggggttttta
     4741 ttatctctcg cgggacatta tatgaattgc agtggcgcgg aaattttgcc gatacctggt
     4801 atctttactg tgggccgatg gtttttatct gcgcaatcac gctattgact ctggttaaaa
     4861 acacgcttga tacgcgtacc gttcgcggac ttggcttaat ctcccgccat tcgttgggca
     4921 tatacgggtt ccacgccttg attatccatg cactgcgcac ccgggggatt gagcttaaaa
     4981 actggccaat actcgatatt atttggatct tttgcgcgac gttggcagcg agtttgttac
     5041 tttctatgct ggtacaacga atcgacagaa acagactagt gagttaagta aaaggccggt
     5101 cacattggac tgaccgggct tatgtgaatt attcgctgta ttcttcctgg gtaacctgtg
     5161 tttcttttgg tgggtttatt ccggcatcac gaatattctt ctgcaccgct actgcaccaa
     5221 acaagcttaa gaagaaagcc agtccataaa aacctttctc gctgagtaat aacgttgcat
     5281 tccacagacc taccatcagt aacgcaacag agataataaa gacagtcagg caggtcatat
     5341 aataaatgga ggtggtcggt atgccttcat acttgtcccg aacggtcttt tgataagacg
     5401 ccgcagaaaa cagtcctaat accagtacgg caaaataata tcctttttca tttaactgca
     5461 tctctgcatt ccatagccct aacagatagg taacgatacc accaacgaga gctatccatg
     5521 acacaatact gaatgccgga gaataggttg atattttgtt gtccatcaca gtattccttc
     5581 attcctgaat gtgtaagagc tttatgttgc tcatgccgag cgaaacaaac gcatttgacc
     5641 aaacaagcag acaaaaaagg cgatagggta gatcaccatt tcataagcat tgataggtgc
     5701 gtttaaaaca ccgataaaca agagaccgat agtgattaac gcaactaact ggcagatatg
     5761 ggtaaaaaaa ttatccagtt gcccgagttt ttctgcgcga agatatgcat agttgccaaa
     5821 agttgctgtc attaatatgc caagaaaata gccttttcca cttaacaatg ggcatgagat
     5881 ccatagccca accagataaa ccagcgcccc gacaacaaat aatagttttg ccacagtctt
     5941 tgaggttttc ataacgatct ccatatctac cagcgataca ttacgagtaa ccaacgaaag
     6001 acaaaactga aaaatgccat taacaaatga ttttcagaat aaattcatac taaatattaa
     6061 ttaattactg agatatatag atgtgaatta tcccccaccc ggtcaggcag aggataacgt
     6121 ttacgccatt aatggcagaa gttgctgata gaggcgacgg aacgtttctc gtcgtggctg
     6181 ataagcggca taacgctgcg catctggtag atgcgactgt tctaacggta actgcggcaa
     6241 caactcaatg agcgatttct ctggattcgc cgcaatctgc gccagccttg ctgcgcccag
     6301 tgctggcccc acatcccccc ccgtacggta atcgagctgc tgaccgctga tatccgccag
     6361 catttgacgc cagtactcac tacgcgcccc gccaccaatc aacgtaacac tttgcggttt
     6421 aatgccgcag gcatgcacga catccatgcc atctgccagc gcataaccca cgccttccag
     6481 cactgctcgc gccagttcat tggggccatg ttgatgagtc aaaccaaaga aaaccccctt
     6541 cgcctgggga ttattgtgtg gcgtacgctc gccggaaaga taaggcagaa accaaactgg
     6601 ctcggcactt tcatcagcct gttgagctgc agcgattaaa gctgggacat tgctcaggcc
     6661 ggttaatttc gcggcccaat ccagacacga cgctgcactc agcattacag acattaaatg
     6721 ccaacgctgc ggtagcgcat ggcaaaagct gtgtacggcg ctttctggct tgcttaaaaa
     6781 cccttcgctg acagcaaaat agacccccga cgtccccagc gataacattg cctgattagc
     6841 atcaaccatt cctacaccaa ccgcaccagc tgcattatca ccaccgcctg cgacaactgg
     6901 caccgccgcc atactccacg ctttcgcaac ttcaggtagc aaagcaccag taatttcgct
     6961 gccttcgtat aatgcgggca tctggtcacg agataagtgg caagcctgca gcatgtcgtc
     7021 actccagtca cgctttgcga catccagcca catggtgcca gctgcgtcag acatatcgct
     7081 ggcaaactcc cccgtcatac gcagacgcaa gtaatctttc ggtaataata ctttgtcgat
     7141 ttgacggaat atctccggct catgccgctg aacccatagc aatttaggcg cagtaaatcc
     7201 gggcatcatc aggttaccgg taatcacccg tgattgcgga actcgcgctt ccagcaaagt
     7261 gcactcttgc gcacagcgcc cgtcgttcca caaaatggca gggcgtaata cccgttgctg
     7321 ggcatccagt aaggttgcac cgtgcatctg gccggcaata cccaatgctt taacgtcctg
     7381 cagagaatgc tgatcgccca gagctttcat tgcgcggtca gttgcctgcc accactgttc
     7441 cgggtcttgt tccgaccaga gtggatgcgg gcgcgaaacg gtcagctttt ccgtttgcga
     7501 agcaaccacc tcaccctgct cgttgagcaa aataactttt acgcccgagg tgccaagatc
     7561 tatcccgata tacatatcga tcgttcctta aaaaaatgcc cggtatcgct accgataacc
     7621 gggccaacgg actgcacagt tagccgttat ttgtcgaaca gataatgatt taccagattt
     7681 tccagttgct cctggcgacc actctgatgc accggagaca aattgtgttc ctgagcatat
     7741 ttggctaaat ctgccagtga catttggcct ttcaggattt gctggcctaa ttcgctattc
     7801 cagccggaat aacgctgcgc gatgcgttta tccagctcgc catcttcaat catgcgtgct
     7861 gcaattttca gcgccaacgc catcgtatcc atcgcgccga tatgaccgta aaacagatca
     7921 tatttatcag tactttgacg acgtactttg gcatcgaagt tcagaccgcc agtggtgaaa
     7981 ccgcctgctt tgagaatttc atacatcacc agcgcattct cttccacact gttcgggaac
     8041 tggtcggtgt cccagcccag ttgtgcatcg ccacggttgg cgtcgacaga accgaacagg
     8101 ccaagcgcaa tggcggtggc tatttcatga tggaaagagt gacctgccag cgtcgcgtgg
     8161 ttagcttcaa tattcagttt aatctctttt tccagaccaa actgtttcag gaagccatag
     8221 accgtcgcag catcgtaatc atattgatgt ttggtcggtt cttgcggttt cggttcgata
     8281 agcaacgtgc cctggaaacc gattttatgt ttatgctcaa ccaccatctg cataaagcgg
     8341 cccagctgtt cacgctcctg gcgcaagtcg gtatttaaca gcgtttcgta accttcacga
     8401 ccaccccaca ggacatagtt ttcaccgccc aatttatggg ttgcttccat cgctgtaaca
     8461 acttgcgttg ccgcccaact gaagacttca ggatctgggt tcgtcgccgc acccgcgcca
     8521 tagcgagggt ttgtaaagca gttagcagtt ccccacagca gcttcacgcc gctctcttct
     8581 tgcttgcctg ccaggacatc aaccatttgc gcaaaattat tgatgtactc ttttaacgac
     8641 gcgccctcag gggaaacatc cacatcgtgg aagcaataaa atggcacatg taacttgtgg
     8701 aaaaactcaa atgcgacatc tgctttacgc ttcgccaacg ccagtgcctc accaggctgc
     8761 tgccacggac gattaaacgc tcccacacca aacatatccg ccccgttcca gcagaaggtg
     8821 tgccagtagc aggcggcaaa acgcaagtgc tcttccatac gcttgcccaa caccagttcg
     8881 tcgggattgt agtgacggaa tgctaacggg tttgaggatt ttgagccttc ataacgaacg
     8941 cgatcgagct ggtcaaaata ggcttgcata ttgaactcca taatcaggta atgccgcggg
     9001 tgatggatga tgtcgtaata ttgggcactc cctttcagtt gctcaattat gttatttcac
     9061 actgctattg agataattca caagtgtgcg ctcgctcgca aaataaaatg ggatgatgaa
     9121 actgggtaat tcctcgaaga gaaaaatgca ataagtacaa ttgcgcaaca aaagtaagat
     9181 ctcggtcata aatcaagaaa taaaccaaaa atcgtaatcg aaagataaaa atctgtaatt
     9241 gttttcccct gtttagttgc taaaaattgg ttacgtttat cgcggtgatt gttacttatt
     9301 aaagctgtcc tctaactaca gaaggcccta caccatgaaa ataaagaaca ttctactcac
     9361 cctttgcacc tcacttctgc ttaccaacgt tgcggcacac gccaaagaag tcaaaatagg
     9421 tatggcgatt gatgatctcc gtcttgaacg ctggcaaaaa gatcgagata tttttgtgaa
     9481 aaaagcagaa tctctcggcg cgaaagtatt tgtacagtct gcaaatggca atgaagaaac
     9541 acaaatgtcg cagattgaaa acatgattaa ccggggtgtc gatgttcttg tcattattcc
     9601 ttataacggt caggtattaa gtaacgttgt aaaagaagcc aaacaagaag gtattaaagt
     9661 attagcttac gaccgtatga ttaacgatgc ggatatcgat ttttatattt ctttcgataa
     9721 cgaaaaagtc ggtgaactgc aggcaaaagc cctggtcgat attgttccgc aaggtaatta
     9781 cttcctgatg ggcggctcgc cggtagataa caacgctaag ctgttccgcg ccggacaaat
     9841 gaaagtgtta aaaccttacg ttgattctgg aaaaattaaa gtcgttggtg accaatgggt
     9901 tgatggctgg ttaccggaaa acgcattgaa aattatggaa aacgcgctaa ccgccaataa
     9961 taacaaaatt gatgctgtag ttgcctcaaa cgatgccacc gcaggtgggg caattcaggc
    10021 attaagcgcg caaggtttat cagggaaagt agcaatttcc ggccaggatg cggatctcgc
    10081 aggtattaaa cgtattgctg ccggtacgca aactatgacg gtgtataaac ctattacgtt
    10141 gttggcaaat actgccgcag aaattgccgt tgagttgggc aatggtcagg aaccaaaagc
    10201 agataccaca ctgaataatg gcctgaaaga tgttccctcc cgcctgctga caccgatcga
    10261 tgtgaataaa aacaacatca aagacacggt agttaaagac ggattccaca aagagagcga
    10321 gctgtaagcg ttacgccccg gcgaggagcg ggggcgtgat ttctctccat gccgtgtgaa
    10381 ttaattggct taggtggagt cgttatgcct tatctacttg aaatgaagaa cattaccaaa
    10441 accttcggca gtgtgaaggc gattgataac gtcagcttgc ggttgaatgc tggcgaaatc
    10501 gtctcactct gtggggaaaa tgggtctggt aaatcaacgc tgatgaaagt gctgtgtggt
    10561 atttatcccc atggttccta cgaaggcgaa attatttttg cgggagaaga gattcaggcg
    10621 agtcacatcc gcgataccga acgcaaaggt atcgccatca ttcaccagga attggccctg
    10681 gtgaaagaat tgaccgtgct ggaaaatatc ttcctgggta acgaaataac ccataatggc
    10741 attatggatt atgatctgat gacgctacgc tgtcagaagc tgctcgcaca ggtcagttta
    10801 tccatttcac ctgatacccg cgttggcgat ttaggacttg ggcaacaaca actggttgaa
    10861 attgccaagg cacttaataa acaggtgcgt ttgttaattc tcgatgagcc gacagcctca
    10921 ttaactgagc aggaaacgtc ggttttactg gatattattc gcgatctaca acagcacggt
    10981 atcgcctgta tttatatttc gcacaaactc aacgaagtca aagcgatttc cgatacgatt
    11041 tgcgttattc gcgacggtca gcacattggc acgcgtgatg ctgctggaat gagtgaagac
    11101 gatattatca ccatgatggt cggacgagag ttaaccgcgc tttaccctaa tgaaccacat
    11161 accaccggag atgaaatatt acgtattgaa catctgacgg catggcatcc ggttaatcgt
    11221 catattaaac gagttaatga tgtctcgttt tccctgaaac gtggcgaaat actgggtatc
    11281 gccggactcg ttggtgccgg acgtaccgag actattcagt gcctgtttgg tgtgtggccc
    11341 ggacaatggg aagggaaaat ttatattgat ggcaaacagg tagatattcg taactgtcag
    11401 caagccatcg cccaggggat tgcgatggta cccgaagaca gaaagcgcga cggcatcgtt
    11461 ccggtaatgg cggttggtaa aaatattacc ctcgccgcac tcaaaaaatt taccggtggc
    11521 attagccagc ttgatgacgc ggcagagcaa aaatgtattc tggaatcaat ccagcaactc
    11581 aaagttaaaa cgtcgtcccc cgaccttgct attggacgtt tgagcggcgg caatcagcaa
    11641 aaagcgatcc tcgctcgctg tctgttactt aacccgcgca ttctcattct tgatgaaccc
    11701 accaggggta tcgatattgg cgcgaaatac gagatctaca aattaattaa ccaactcgtc
    11761 cagcagggta ttgccgttat tgtcatctct tccgaattac ctgaagtgct cggccttagc
    11821 gatcgtgtac tggtgatgca tgaagggaaa ctaaaagcca acctgataaa tcataacctg
    11881 actcaggagc aggtgatgga agccgcattg aggagcgaac atcatgtcga aaagcaatcc
    11941 gtctgaagtg aaattagccg taccgacatc cggtggcttt tccgggctga aatcactgaa
    12001 tttgcaggtc ttcgtgatga ttgcagctat catcgcaatc atgctgttct ttacctggac
    12061 caccgatggt gcctacttaa gcgcccgtaa cgtctccaac ctgttacgcc aaaccgcgat
    12121 taccggcatc ctcgcggtag gaatggtgtt cgtcataatt tctgctgaaa tcgacctttc
    12181 cgtcggctca atgatggggc tgttaggtgg cgtcgcggcg atttgtgacg tctggctagg
    12241 ctggcctttg ccacttacca tcattgtaac gctggttctg ggattgcttc tcggtgcctg
    12301 gaacggatgg tgggtcgcgt accgcaaagt cccttcattt attgtcaccc tcgcgggcat
    12361 gttggcattt cgcggcatac tcattggcat caccaacggc acgaccgtat cccccaccag
    12421 cgccgcgatg tcacaaattg ggcaaagcta tctacctgcc agcactggct tcatcattgg
    12481 cgcgcttggc ttaatggctt ttgttggctg gcaatggcgc ggaagaatgc gccgtcaggc
    12541 tttgggtttg caatctcccg cctctaccgc agtagttggt cgccaggctt taaccgctat
    12601 catcgtatta ggcgcaatct ggctgttgaa tgattaccgt ggcgttccca ctcctgttct
    12661 gctgctgacg ttgctgttac tcggcggaat gtttatggca acgcggacag catttggacg
    12721 acgcatttat gccatcggcg gcaatctgga agcggcacgt ctctccggga ttaacgttga
    12781 acgcaccaaa cttgccgtgt tcgcgattaa cggattaatg gtagccatcg ccggattaat
    12841 cctcagttct cgacttggcg ctggttcacc ttctgcgggg aatatcgccg agctggacgc
    12901 aattgcagca tgtgtgattg gcggcaccag tctggcaggc ggcgtcggga gcgtggctgg
    12961 agcggtaatg ggggcattca tcatggcttc actggataac ggcatgagta tgatggatgt
    13021 accgaccttc tggcagtata tcgttaaagg tgcgattctg ttgctggctg tatggatgga
    13081 ctccgcaacc aaacgccgtt cttgattttg ataaaaattt tctcaaagcc ggtaacgtat
    13141 taccggtttt gagtttttgc atgattcagc aggaaaagaa ccatgtttac taaacgtcac
    13201 cgcatcacat tactgttcaa tgccaataaa gcctatgacc ggcaggtagt agaaggcgta
    13261 ggggaatatt tacaggcgtc acaatcggaa tgggatattt tcattgaaga agatttccgc
    13321 gcccgcattg ataaaatcaa ggactggtta ggagatggcg tcattgccga cttcgacgac
    13381 aaacaaatcg agcaagcgct ggctgatgtc gacgtcccca ttgttggggt tggtggctcg
    13441 tatcaccttg ccgaaagtta cccacccgtt cattacattg ccaccgataa ctatgcgctg
    13501 gttgaaagcg catttttgca tttaaaagag aaaggcgtta accgctttgc tttttatggt
    13561 cttccggaat caagcggtaa acgttgggcc actgaacgcg aatatgcatt tcgtcagctt
    13621 gtcgccgaag aaaagtatcg cggagtggtt tatcaggggt tagaaaccgc gccagagaac
    13681 tggcaacacg cgcaaaatcg gctggcagac tggctacaaa cgctgccacc gcaaaccggg
    13741 attattgccg ttactgacgc ccgggcgcgg catattctgc aagtatgtga acatctacat
    13801 attcccgtac cggaaaaatt atgcgtgatt ggcatcgata acgaagaact gacccgctat
    13861 ctgtcgcgtg tcgccctttc ttcggtcgct cagggcgcgc ggcaaatggg ctatcaggcg
    13921 gcaaaactgt tgcatcgatt attagataaa gaagaaatgc cgctacagcg gattttggtc
    13981 ccgccagttc gcgtcattga acggcgctca acagattacc gctcgctgac cgatcccgcc
    14041 gttattcagg ccatgcatta cattcgtaat cacgcctgta aagggattaa agtggatcag
    14101 gtactggatg cggtcgggat ctcgcgctcc aatcttgaga agcgttttaa agaagaggtg
    14161 ggtgaaacca tccatgccat gattcatgcc gagaagctgg agaaagcgcg cagtctgctg
    14221 atttcaacca ccttgtcgat caatgagata tcgcaaatgt gcggttatcc atcgctgcaa
    14281 tatttctact ctgtttttaa aaaagcatat gacacgacgc caaaagagta tcgcgatgta
    14341 aatagcgagg tcatgttgta gcgatgatga gaattgtcgg cgtcgcatca ggtaactcag
    14401 cacaaccacc tgatgcaagc aaaagcctta tccgactgac aagtcggata aggctcgagg
    14461 aaatgcaatt acatatgcgc cgcgattaac cgttggttat cctggtacat tgcgaacagg
    14521 tagttgttat aactcttccc tttagtcgag tagcccttca gcttgtgaat catcgctgtg
    14581 gcagtcactt cctgatccgc tttacgcagc tgtgcacgcg atttacggaa cgaagagtaa
    14641 gccggatgag tattcaggtt agtgacatag gcgctcaccg attctttgac agaactaaac
    14701 tgtgagtacc ctttcacttt acccggcgca ttggtacaac gtcctttcat gcatttcatg
    14761 ccgaacagat tgttgttgtt gcgcgccagc tttgacgttc cccaaccgct ttctgctgca
    14821 gccatcgtcg ccaccatact ggtagggata atgtctacgc gttcaagcaa ggtattccac
    14881 gggatttttc gcgtattacc agaccacttc accttgtagc gtttggcgat gtctttcaga
    14941 cgcgcacgct cagcaggtga ccattggccc tgatactgtt ttgaaatcag ccagttacgt
    15001 tccgcagtaa tggccgcatt ttggctggta atgtaaggca ttacagtccg gagaaacgcc
    15061 tttttccttg gtgttccgga agggtatttt cgcaaatcag gaagtgaact actctttgca
    15121 ctattgcgag aatactcttg tttactgctt acctgtttat tactagcttt agttaagtgg
    15181 gacttttgac tcgctgttgt tgtgtgcgtc ttcgctaaca cctcactcga aaacaccaga
    15241 gtgagtaaca taagaatcat cgccccatat cgtcgtatgg gagtcaaaat catcaggtct
    15301 cctggtcgga tttaatcatt ccaacacctt atatttttca caaatttgag agttgaatct
    15361 caaatcatat caaaaatagc tgtcaagagc accccaagga atagtccaaa tctgaaacta
    15421 tgtcacgagt taacgattca gattggcgct aaatcgcaga aaatgtgggg tttatcgcaa
    15481 aattcagccg ttttttgtgc gcgatcgctc acccttgctt ctcatcctgt ggacttaccg
    15541 ctcagggatg agttttgtct ggcttatcgc tggcaaactg tctgaaatcg cagcaataag
    15601 gactcatccg ccatgaaact cgccgcctgt tttctgacac tccttcctgg cttcgccgtt
    15661 gccgccagct ggacttctcc ggggtttccc acctttagcg aacaggggac gggaacattt
    15721 gtcagccacg cgcagttgcc caaaggtacg cgtccactaa cgctaaattt tgaccaacag
    15781 tgctggcagc ctgcggatgc gataaaactc aatcagatgc tttccctgca accttgtagc
    15841 aacacgccgc ctcaatggcg attgttcagg gacggcgaat atacgctgca actagacacc
    15901 cgctccggta caccaacatt gatgatttcc atccagaacg ccgtcgaacc ggtagcaagc
    15961 ctggtccgtg aatgcccgaa atgggatgga ttaccgctca cactggatgt cagcgccact
    16021 tttccggagg gagccgccgt ccgggattat tacagccagc aaattgcgat agtgaagaac
    16081 ggtcaaataa cgttacaacc cgctgccacc agcaacggtt tactcctgct ggaacgggca
    16141 gaaactgacg cccctgcccc tttcgactgg cataacgcca cggtttactt tgtgctgaca
    16201 gatcgtttcg aaaacggcga tcccagtaat gaccagagtt acggacgtca taaagacggt
    16261 atggcagaaa ttggcacttt tcacggcggc gatttacgcg gcctgaccaa caaactggat
    16321 tacctccagc agctgggcgt caatgcttta tggataagtg ccccatttga gcaaattcac
    16381 ggctgggtcg gcggcggtac aaaaggcgat ttcccgcatt atgcctacca cggttattac
    16441 acacaggact ggacgaatct tgatgccaat atgggcagtg aagccgatct acggacactg
    16501 gttgatagcg cgcatcagcg cggtattcgt attctctttg atgtcgtgat gaaccacacc
    16561 ggctatgcca cactggcgga tatgcaggag tatcagtttg gcgcgttata tctttctggt
    16621 gacgaagtga aaaaaacgtt gggtgaacgc tggagcgact ggaaacctgc cgccgggcaa
    16681 acctggcata gctttaacga ttacattaat ttcagcgaca aaacaggctg ggataaatgg
    16741 tggggaaaaa actggatcag aaccgatatc ggcgattacg acaatcctgg atttgacgat
    16801 ctcactatgt cgctggcctt tttgccggat atcaaaaccg aatcaacgac cgcttctggt
    16861 ctgccggtgt tctataaaaa caaaacggat acccacgcta aagtcatcga cggctttacc
    16921 cctcgcgatt acttaaccca ctggttaagt cagtgggttc gcgactatgg gattgatggt
    16981 tttcgggtcg acaccgccaa acatgttgag ttgcccgcct ggcagcaact gaaaaccgaa
    17041 gccagcgccg cgcttgtcga atggaaaaaa gcgaatcccg ataaagcgtt agatgacaaa
    17101 ccgttctgga tgaccggtga agcctggggc cacggcgtga tgcaaagtga ctactatcgc
    17161 cacggcttcg atgcgatgat caatttcgat tatcaggagc aggcggctaa agcagtcgat
    17221 tgtctggcgc agatggatac gacctggcag caaatggcgg agaaattgca ggatttcaac
    17281 gtgttgagct acctctcgtc gcatgatacc cgcctgttcc gtgaaggggg cgacaaagca
    17341 gcagagttat tattagcgcc aggcgcggta caaatctttt atggtgatga atcctcgcgt
    17401 ccgttcggcc ccaccggttc ggatccactg caaggcacgc gttcggatat gaactggcag
    17461 gatgttagcg gcaaatctgc cgccagcgtc gcgcactggc agaaaatcag ccagttccgc
    17521 gcccgccatc ccgcaattgg cgcgggcaaa caaacgacac tttcgctgaa gcagggctac
    17581 ggctttgttc gtgagcatgg cgacgataaa gtgctggtca tctgggctgg gcaacagtaa
    17641 cttttccggc ttcccgttcg tcagtacctc gggaagccgc caaccaggat aaaatgtcag
    17701 acctaatcag cgttgcagga taaagcaccg ctccctcttc aacagagcga tttgcacccc
    17761 agcaaatgta gcgttattgt taccttcctt gctacagagt tcgacagata tcccgctatg
    17821 acattctccc tttttggtga caaatttacc cgccactccg gcattacgct gctgatggaa
    17881 gatctgaacg acggtttacg cacgcctggc gcgattatgc tcggcggcgg taatccagcg
    17941 cagatcccgg aaatgcagga ctacttccag acgctactga ccgacatgct ggaaagtggc
    18001 aaagcgactg atgcactgtg caattacgac ggtccacagg ggaaaacgga gctactcaca
    18061 ctgcttgccg gaatgctacg cgagaagttg ggttgggata tcgaaccaca gaatattgca
    18121 ctaacaaacg gcagccagag cgcgtttttc tacttattta atctgttcgc cggacgccgt
    18181 gccgatggtc ggatcaaaaa agtgctgttt ccgctggcac cggaatacat tggctacgct
    18241 gacgccggac tggaagaaga tctgtttgtc tctgcgcgtc cgaatattga gctgctgccg
    18301 gaaggccagt ttaaatacca cgtcgatttt gagcatctgc atattggcga agaaaccggg
    18361 atgatttgcg tctcccggcc gacgaaccca acaggcaatg tgattactga cgaagagttg
    18421 ctgaagcttg acgcgctggc gaatcaacac ggcattccgc tggtgattga taacgcttat
    18481 ggcgtcccgt tcccgggtat catcttcagc gaagcgcgcc cgctgtggaa tccgaatatc
    18541 gtactgtgca tgagtctttc caagctgggt ctaccaggct cccgctgcgg cattatcatc
    18601 gccaatgaaa aaatcatcac cgccatcacc aatatgaacg gcattatcag cctggcacct
    18661 ggcggtattg gtccggcgat gatgagtgaa atgattaagc gtaacgatct gctgcgcctg
    18721 tctgaaacag tcatcaaacc gttttactac cagcgtgttc aggaaactat cgctatcatt
    18781 cgccgctatt taccggaaga tcgctgcctg attcataaac cggaaggagc cattttcctc
    18841 tggctatggt ttaaggattt gcccattacg accgagcagc tctaccagcg cctgaaagca
    18901 cggggcgtgc tgatggtgcc ggggcacaac ttcttcccag ggctggataa accgtggcca
    18961 catacacatc aatgtatgcg catgaactac gtgccggagc cggagaaaat tgaggcgggt
    19021 gtgaagattc tggcagaaga gatagaaaga gcctgggctg aaagtcacta aaaattgcct
    19081 gatgcgctac gcttatcagg cctacatgat ttctgcaata tattgaattt ggtagaattt
    19141 gtaggccgga taaggcgttt tagccgcatc cggcataaac aaagcgcact atccctgcgt
    19201 tgagaaacgc cggaaaaacg tttcaaacag gctgctgccg tagccgatgt cgctgcactt
    19261 tctcgacatc catgcactgc aacgcctttg tcgggcaggc ttcaacacag gccggtcccg
    19321 tcgcccgatg ccagcacaga tcgcacttaa tcgccctcgc ctttttgcgc gaagaaacga
    19381 cctccatcgc accaaacggg caagccagca tacagctttt acagccaatg caacgtgatt
    19441 gttcaacgaa aatatgccca tgttcgcggc ttatcgcgtc aacagggcag acattcgcgc
    19501 acggtgcatc ttcacactga tggcaggcta cagccgtggt ccatgtgtgg tctttaatga
    19561 cacgaatacg ggaaataaac tcgtctggtg acaacgcagc gcaatcctga ttctcctgat
    19621 gcgacactgc gcaagccact tcacaggtac ggcaaccgat acatttcgtc gcatccgcaa
    19681 taataaaccg gttcatgatc actcctttgc actaaaacag cagagtgcca gagagcgcgt
    19741 ctctactgct ttgatccaac gaaggaaatg ttaaagtttt ggcacttgtc caaagcgtta
    19801 tgtgatcgcg ccctggtcgt ccctgacagt aaatcccagt tcattagaaa tagcctgcgc
    19861 ggtttcacgc agtggtttca ggagattttt ctcccccacc tgtttcagac gtgatgtcga
    19921 aagcgaaatc gacacggcgt acggcacccg cccatgaata tcaaacaccg gaacagcaat
    19981 acaggagacg ccgagttcgt tttcttctct gtccatcgcc gctccgcttt cacgaatgtg
    20041 cgccagttcg tcgaacatcg cgggcagctc ggtaatggta ttgcgggtta acggctggat
    20101 ctcatgctga tggctttccc agtatgactt cacgtagtcc gggtggccaa acgccatgta
    20161 gatcttgccc attgcggaac agtagagcgg catatgctgg ccaatatagg cacgggttcg
    20221 cagcatcccg gttgtgggtt ccagcttata aatcaaaata gcgtgatcgt cttcgcggct
    20281 ggagaagtta atagtttcac cagtggcgat gttcagtgcc tcaagatgcg gagcggcgat
    20341 atgaatgata ttcagcgaag acagcgcctt ctgcccgacg gcaataaatt tggtggtcag
    20401 gcgataactc cctgcggcgg gcgcggtggt cacatagccg caggattgta atccctgcaa
    20461 taagcgatgg acggtactct tatttaaacc agccagctcc gaaagatgcg ccaacggaca
    20521 accgtttgga tagttgctca aaatctcaat cagcatcaac ccgcgaaaca gactctggct
    20581 tccggctgga cgctcttttt cctgcgccat ctcgttctct ttttttccca tcacttcttt
    20641 ccccattttg tcgcgtcaag atggtagcgc aaagtgtgtc gtagttcacg atctcgacag
    20701 ataatttata accacttgat ttttaagttt tttgaaattc atcaatcaga ttgctttgtt
    20761 aaaaagcgat cgatatattt gaaatcagat ttcgcatatt gaaattttaa gccaaaaaag
    20821 cgatcaaaaa aacaaggaag ccagggatga aagtgacatt tgagcagtta aaagcagcct
    20881 ttaatcgagt cttaatttca cgcgacgttg acagcgaaac ggctgatgcc tgtgccgaaa
    20941 tgttcgcccg caccaccgaa tccggcgttt attctcacgg cgtcaaccgt ttccctcgtt
    21001 tcattcaaca actggaaaac ggcgatatca ttcctgatgc ccaacccaaa cgtataacca
    21061 gcctcggcgc aattgaacag tgggacgccc agcgttcgat cggtaacctg acagcgaaaa
    21121 agatgatgga tcgcgccatt gaactggctg ccgatcacgg tattggtctg gtggcactac
    21181 gtaatgccaa ccactggatg cgcggcggca gctacggctg gcaggcggcg gaaaaaggct
    21241 atattggcat ttgctggacc aactccatcg ccgtaatgcc gccgtggggc gcaaaagagt
    21301 gtcgcatcgg cactaacccg ctgatcgtcg ccattccttc taccccaatc accatggtcg
    21361 atatgtcgat gtcgatgttc tcttacggca tgttagaagt taaccgtctg gcaggccgtc
    21421 agctcccggt cgatggtggc tttgatgatg agggcaattt gaccaaagaa cctggcgtta
    21481 tcgagaagaa tcgccgcatt ttgccgatgg gttactggaa aggttcgggc atgtcgattg
    21541 tgctggatat gatcgccact ctcctttctg acggcgcatc ggttgccgaa gtcacccagg
    21601 acaacagcga cgaatacggc atttcacaaa tttttattgc cattgaagta gataagctta
    21661 tcgacggccc cacccgcgat gccaaactgc aacgcatcat ggattacgtt accaccgctg
    21721 aacgcgctga cgaaaatcag gccattcgct tacccggcca cgaatttact accctgctgg
    21781 ccgaaaaccg ccgtaacggt attaccgttg atgacagcgt gtgggcaaaa atccaagcgt
    21841 tataaggaga taacccatga tttttggaca tatcgcgcag cctgacccgt gccgtttgcc
    21901 tgccgccatt gaaaaggcgc ttgattttct gcgcaccacc gacttcaacg ccctggaacc
    21961 gggcgttgtc gaaatcgacg gcaagaatat ttatgcgcaa attatcgatt taaccacacg
    22021 cgaagcggcg gaaaaccgtc cggaagtgca tcgtcggtat atcgatattc agtttctggc
    22081 gtggggtgaa gagaaaatcg gcattgctat tgatacggga aataataaag tcagcgaatc
    22141 actattagag cagcgcgata ttatttttta tcacgatagt gaacatgaat catttataga
    22201 aatgataccg ggcagctacg ctattttctt cccgcaggat gttcaccgac caggttgtat
    22261 tctgcaaaca acctctgaga tacgaaaaat tgtggttaaa gtcgcattaa cagcgctgaa
    22321 ttaatcgaat aatcgtctac gccagaacgc ctgggtaatg ttattgctct aactatatta
    22381 ataatttagg ttgctgcaca tgcaacttga attatgacag acatagcctc aaggaatagc
    22441 tatgaaaaaa atacttgaag caatactggc gattaatctc gccgtacttt cctgtattgt
    22501 gtttataaac attattttaa gatatggttt tcagacaagc attttatctg tagatgaatt
    22561 gtcacgttat ttatttgtct ggttaacatt tattggcgcg attgtggctt ttatggataa
    22621 cgcccacgtt caggtcacct ttctggtgga gaaactctcc cccgcatggc agcgacgagt
    22681 cgctctggtt acccattcct taatcttatt tatttgtggc gcactggcct ggggcgcaac
    22741 gctaaaaaca attcaggact ggagcgatta ttcaccgatc ctcggtttgc ccatcggcct
    22801 gatgtatgcc gcttgtctgc ccaccagcct cgttatcgca ttctttgaac tgcgtcattt
    22861 atatcaactc atcacgcgta gcaattctct aacgtcgcca ccgcaaggag cttaatcatg
    22921 gctgtgctga tttttctggg ctgtctgttg ggtggtattg ctatcggttt gcctattgcc
    22981 tgggcactgt tgttgtgcgg agcggcgtta atgttctggc tggacatgtt tgatgtccag
    23041 atcatggcac aaacgctggt gaacggagcc gacagcttct ccctgctggc gattccgttc
    23101 tttgtgctgg cgggtgaaat catgaatgcg ggcggcttgt caaaacgcat tgttgacctg
    23161 ccgatgaaac tggtggggca taaacctggc gggttgggct acgtcggcgt gctggcggct
    23221 atgattatgg cgagcctttc tggttctgct gttgctgata ccgccgccgt tgccgcgcta
    23281 ttagtgccga tgatgcgcag cgctaactac ccggtcaacc gggctgcggg gctgattgcc
    23341 tctggcggca ttatcgcgcc aattattccc ccttccattc cgtttattat tttcggcgtt
    23401 tccagcggat tatccatcag taagctgttt atggcgggca ttgcccctgg catgatgatg
    23461 ggcgcaacgc tgatgcttac ctggtggtgg caggccagtc gccttaattt accccgccag
    23521 caaaaagcaa cgatgcagga aatctggcac tcttttgtct ccggtatctg ggcgctgttt
    23581 cttccagtca tcattattgg cgggtttcgc tccggtctgt ttacccctac cgaagccggg
    23641 gccgttgctg ctttctatgc gctgtttgtc gccacagtta tttaccgtga aatgaccttc
    23701 gccacacttt ggcatgtgct cattggcgca gcgaaaacga cctcagtggt gatgtttctg
    23761 gtagcctcgg cacaagtttc cgcctggctg atcaccattg ctgaactgcc gatgatggtt
    23821 tctgatttac tgcaaccgct ggtcgattca ccgcgtctgc tgtttatcgt cattatggtg
    23881 gctatcctga ttgtcggcat ggtcatggat ttaacgccga cggtattaat tcttaccccg
    23941 gtattaatgc ctttagttaa agaggcagga atcgatccga tttatttcgg tgtcatgttt
    24001 atcattaact gctcaatcgg cttaattaca ccgcctatcg gtaatgtgct taacgttatt
    24061 tcaggggtgg caaaactcaa attcgatgat gcagtcagag gcgttttccc ttacgtcctg
    24121 gttttatatt cattattagt cgtatttgtt tttattcccg atctcatcat cctccctttg
    24181 aaatggatta attaaaagga aaatattatg aaattacgct ctgtaaccta cgcattattc
    24241 attgctggcc tggctgcatt tagtacatct tcactggcgg cgcaatcttt acgtttcggt
    24301 tatgaaacct cacaaaccga ctcgcaacat attgcggcga aaaaattcaa tgatttattg
    24361 caggagagaa ccaaaggcga gctgaaatta aaactgttcc cggacagtac tctcggtaac
    24421 gcacaggcga tgatcagcgg cgtacgtggc ggcaccatcg atatggaaat gtccggctcg
    24481 aataactttg ccgggttatc accagtgatg aacttgcttg atgtcccttt cctgttccgc
    24541 gataccgctc acgcgcataa aacgctcgac ggcaaagtcg gtgatgatct gaaagcctca
    24601 cttgaaggta aaggactaaa agtactggcc tactgggaaa acggctggcg cgatgtcacc
    24661 aactcgcgcg caccggttaa aacccccgcc gacctgaaag ggctgaaaat tcgcaccaac
    24721 aatagcccga tgaatatcgc cgcattcaaa gtctttggcg ctaacccgat cccgatgccg
    24781 tttgccgaag tctataccgg gctggaaacg cgcactatcg acgctcagga acacccgatc
    24841 aacgtcgtct ggtcagcaaa atttttcgaa gtacagaagt acctttctct gacgcaccac
    24901 gcctattcgc cacttctggt ggtgatcaac aaagcgaagt ttgatggctt aagcccggag
    24961 ttccagcagg cgctagtttc atctgcacag gaagcgggta actaccagcg caaactggtt
    25021 gccgaagatc agcaaaaaat catcgacggc atgaaagaag cgggcgtgga agtcatcacc
    25081 gatctcgacc gcaaagcctt tagcgacgca ctgggtactc aggtccgcga catgtttgtt
    25141 aaagatgttc cgcagggcgc tgagctgctg aaagccgtgg atgaggtgca ataaaccatg
    25201 acgcaatact ggctggggtt agattgtggc ggtagctggc tgaaagccgg gctgtatgac
    25261 cgcgaaggcc gggaggcagg cgtgcagcgc ctgccgctgt gcgcattaag cccgcagcca
    25321 ggttgggcag agcgcgatat ggcagaactg tggcaatgct gcatggctgt cattcgcgcc
    25381 ctgcttactc attctggtgt tagcggggaa caaattgtcg gtatcggcat ctccgcacag
    25441 ggaaagggct tgtttttgct ggataaaaac gacaagccgc tcggtaacgc cattttgtcc
    25501 tcggatcgcc gggcgatgga aatcgttcgt cgctggcagg aagatggcat cccggaaaaa
    25561 ctctacccgc tgacgcgaca aaccttgtgg accgggcatc cggtgtcgct gttacgctgg
    25621 ctgaaagagc acgaaccgga acgctacgcg caaattggct gcgtgatgat gacgcacgac
    25681 tacctgcgct ggtgtttaac cggcgtcaaa ggttgcgaag agagcaatat ttctgagtcc
    25741 aatctctaca acatgagtct tggggaatat gacccgtgcc tcaccgactg gctggggatc
    25801 gctgaaatca accacgccct gccacttgtt gtcggatctg ccgaaatctg cggggagatc
    25861 accgctcaga cagccgcact gaccggtctg aaagcgggta cgcccgttgt cggcggcctg
    25921 tttgatgtgg tttccaccgc actctgcgcc gggatcgaag acgaatttac cctcaatgcg
    25981 gtgatgggca cctgggcggt gaccagcggc ataacccgtg gtttacgtga cggtgaagcg
    26041 catccgtatg tctatggtcg ctacgttaac gatggtcaat ttatcgttca tgaagcaagc
    26101 cccacctctt ccggcaatct cgaatggttt accacacagt ggggagaaat ctcgtttgat
    26161 gagatcaacc aggccgttgc cagcttgccg aaggccgggg gcgatctctt tttcctgccg
    26221 ttcctgtacg gcagcaacgc tggactcgag atgaccagcg gtttctacgg gatgcaggcc
    26281 attcacaccc gcgtacacct gttgcaggcc atttatgaag gcgtggtgtt cagccatatg
    26341 acccatctca accggatgcg cgaacgtttt actgatgtgc acaccctgcg cgtcactggc
    26401 ggtccggcgc actccgatgt ctggatgcaa atgctggcgg acgtcagcgg tctgcgtatc
    26461 gagctgccgc aggtggaaga aaccggctgc tttggtgcgg cacttgccgc ccgcgtcggc
    26521 accggagttt atcgcgattt cagcgaagcc caacgtgatt tacagcaccc ggtacgcacc
    26581 ctgctgccgg atatgaccgc ccatcagctt taccaacaaa aataccaacg ttatcagcat
    26641 ctcattgccg cacttcaggg ctttcacgcc cgcatcaagg agcacacatt atgagccgac
    26701 cacttctgca actggccctc gaccactcat cacttgaaga cgcacagcgc gacgtgacgc
    26761 tgttaaaaga cagcgtcgat atcgtcgaag cgggcaccat tctctgttta aacgaagggc
    26821 ttggcgcagt gaaagccttg cgcgaacagt gcccgaacaa aatcatcgtc gctgactgga
    26881 aggtcgccga tgccggtgaa acgctcgcgc aacaggcgtt tggcgcaggc gctaactgga
    26941 tgaccatcat ctgcgccgca ccgctcgcga cggtggaaaa aggccacgcg atggcgcaac
    27001 gctgcggtgg tgaaattcag atcgagttgt tcggtaactg gacgctggac gacgcccgcg
    27061 actggcatcg tattggcgtg cggcaggcca tttatcatcg cggtcgcgac gcgcaggcca
    27121 gcgggcaaca gtggggcgag gccgatctgg cacgcatgaa ggcgctttcc gatatcggcc
    27181 ttgagctttc cattaccggc gggattaccc ctgctgacct gccgctgttt aaagatattc
    27241 gcgtgaaagc gtttattgcc gggcgagcac tggcaggcgc agcaaatccg gtgcaggtgg
    27301 ctggcgattt ccatgcacaa attgatgcca tctggggagg tgcgcgtgcg taatcatccg
    27361 ttagggattt atgaaaaagc gctggcgaaa gatctctcct ggccggagcg gctggtgctg
    27421 gcaaaaagct gtggctttga ttttgtcgaa atgtcggtgg atgaaaccga cgaacggctc
    27481 tcacgtcttg actggagcgc cgcacaacgt gcttcgctgg tcactgcgat gatcgaaacg
    27541 ggtgttggga tcccgtcgat gtgcctgtcc gcacatcgtc gctttccttt tggtagccgt
    27601 gacgaggcag tgcgcgaacg ggcgcgggaa atcatgagta aagcgattcg cctggcgcgc
    27661 gatctcggca ttcgcaccat ccagctggcg ggctacgacg tctattacga agaccacgac
    27721 gaaggcaccc ggcaacgttt tgctgaaggg ctggcgtgga cagtcgaaca ggcggcggca
    27781 tcacaagtaa tgctggcggt ggagattatg gatactgcgt ttatgaactc catcagcaaa
    27841 tggaaaaagt gggacgagat gctcgcctcg ccgtggttca ccgtctaccc ggacgtcggc
    27901 aacctcagcg cctggggcaa tgatgttccc gccgaactga aactgggcat tgaccgtatc
    27961 gccgcgatcc acctgaaaga tactcaacca gtcaccgagc aaagccccgg acagttccgt
    28021 gatgtgccgt ttggcgaagg ctgcgtcgat ttcgttggca tcttcaaaac gctgcatgaa
    28081 ctgaactatc gcggttcttt cctgattgag atgtggaccg aaaaagccaa agagccggtg
    28141 ctggagatta ttcaggcgcg gcgctggatt gaagcgcgta tgcaggaggc aggttttata
    28201 tgttagagca actgaaagcc gacgtgctgg cggcaaatct ggcacttccc gctcaccatc
    28261 tggtgacgtt cacctggggc aatgtcagcg cggtagacga agcgcggcaa tggatggtaa
    28321 tcaaaccttc cggcgtcgag tacgacgtga tgaccgccga cgatatggtg gtggttgaga
    28381 tagccagcgg taaggtggtg gaaggcagca aaaagccctc gtccgacacg ccaacgcatc
    28441 tggcgcttta ccgtcgctat gccgaaatcg gtggcattgt gcatacccac tcgcgccacg
    28501 ccaccatctg gtcgcaggcc gggctggatc tccccgcctg gggcaccacg cacgccgatt
    28561 atttctatgg tgccatcccc tgcacgcgac agatgaccgc agaggagatt aacggcgaat
    28621 atgaatatca gaccggcgaa gtgatcattg aaactttcga agaacgcggc aggagtccgg
    28681 cacaaatccc ggcggtgctg gtgcattctc acggcccgtt cgcctgggga aaaaatgccg
    28741 ccgatgcggt gcataatgcc gtagtgctgg aagagtgcgc ctatatgggc ctgttctcgc
    28801 gccagcttgc gccgcagctc cctgcgatgc aaaacgaact gttagataag cactacctgc
    28861 gtaagcatgg ggccaatgcc tattacgggc agtaatccct cacgccgggg cttcattgcc
    28921 ccggcactac gaattgatat gttccttgct gtaacgtcgc ttccacgcag ctggcgttaa
    28981 accggtgtgt ttgcgaaaaa tctgtcggaa atagccaacg tcattaaacc cgcacaacgc
    29041 agccacttca gtgagcgatc gcgcttcgct aattaacagc ttttcggctt cgcgcactcg
    29101 ctggcgatga attgcttctg ttaacgtcag atgaaacacc cgccggtaga cgcgcccaag
    29161 ataatcggca ttgcagtaca gctcgcgggc taaaagcgcc gtagaaagcg ggcgatgata
    29221 ttgcgtgcga atcagtttct gcgctttcca ggcaagcgtg gtacctgcgg catcggcctg
    29281 ttcatcaaga cgcgcgtcac gggtgagctg ctgcaaaatc agcagcacaa taaactccag
    29341 cgccggagtg cgttgatgct tttcctgttc gcttaagaac tggcgaaaca gggccaccag
    29401 cgcctgcgga tcggcaacct tcgtttgttg cggcacgcta agatgagtga gccagcgcgg
    29461 gtcgctatcc ggcgctttaa cctcaaaatg cagccagtaa aatttgagat cggcgggaaa
    29521 atcctctacc ccaacatgct gtcggttcgg ccagagtaat aaactctctc ccgcatcgac
    29581 cagaaatatc cgctcttctt cctgaatcgt taatcgacct ttttcgacaa agataatttc
    29641 ccatgactcc agccgccttg ccgggtgcct ccctacgcca cgggaaataa ataatccacc
    29701 attttgcact ttaatcggta acgtcatgga taattcgagc attgatatgt aatttcccgc
    29761 ttattcatta atgattaaac acattacctt ttgattatta atcaaaaaca ttaatttcac
    29821 ctgatccatt tcacactttt aaattcaggt cggaatcgtc accttttttt actcttttct
    29881 cgccttgttg atattcccgt tcacggcaga attaatcagg tcattgcaat aaaactaaaa
    29941 attgctccct attcacgagg aatatctatg acctctacac cgattactgc cgctgatatc
    30001 gctaaaggta aaattgacga tgcgttatct gtacgggaaa aaataggcta cggcctgggt
    30061 gacgcaggcg gcaccgtaat aacttgcctg atcatgaact ttctcacctt tttctacacc
    30121 gacgtttttg gcttaactcc ggcgctggtc ggcacgctgt ttattgcact gcgcgtgttt
    30181 gatgccatct ccgacccggt gatgggcgtc attgccgacc ggacgcaaag tcgctggggg
    30241 cgctttcgtc cgtggcagtt atggattgcc attcccatcg gcattatcgg catcctgacg
    30301 ttcaccgtgc cagatgccag catgggagta aaaatcgcct gggcgttcgg tacttacctg
    30361 ctcctttcag tcggttatac cgccatcaac gtaccgtact gcgcgctgat caacaccatg
    30421 accacccgcc acaatgaagt gatctcctgc cagtcctggc gattcgttct ctgcggcgta
    30481 gcgggatttc tggtttcggt tggcctgccg tggatggtgg atctcttcgg tcagggtaat
    30541 gccgcgcgcg gctatcaact gggcgtcggc gtgctgtgcg ccattgccgt ggtgatgttc
    30601 ctgtgctgtt tcttctgggt tcgtgaacgg gtgccactct ccacaatggg gaaatttacc
    30661 ctgcgcgaac atcttgccgg gctgcggaac aacgaccaac tgctgctgat gctggtcatg
    30721 tctttcctgc tgattaacgt ctttaatatt cgcggcggtg ggtatatgta cttcattacc
    30781 tacgtcttac aaggcagtac gggctacacg tcgctgttct tcaccatggt caccttcgcc
    30841 tccattatcg gctcggtgat tgtcagcccg ttaacgcggc gtttcgatac cgtcaaaatt
    30901 tactactaca ccaacctgct cctcgctgcg ctggcggtgt tgatgtggtt cctgccttct
    30961 ggcccggctt atcaaacgct gtggctggcg gtgatcctct gtaatggcgt gattcttggc
    31021 ttcacattgc cactgcactt ctcattgatg gcctttgccg atgactacgg cgagtggaaa
    31081 acccgcgtac gttcttccgg catgaacttc gccttcaatc tgtttttcat caagctggca
    31141 tgggcctcca gcgccgggat catcagcctg ctgtttattt ttgtcgccta ccagccaggc
    31201 gtggaaaacc agaccaccag ttcgcttggc gggatcacgg cgatggaaac attactgcct
    31261 gcgctattcc acctgctgct ggcaggggcg atccgctttt gcaaactcaa taaccctatg
    31321 atgtcacgca ttgctagcga cctgcgtcag cgtcatgtac agccttaagg agcacaagat
    31381 gaacatttcg gaagtcgatc tgcataaact gacggtcagc gatccgttcc tcggtcagta
    31441 ccaacaactg gtccgcgatg tggtgattcc ttatcagtgg gacgccttga acgatcgtat
    31501 cccagaagcg gaacccagcc atgcgattga aaactttcgc attgccgcag gactgcaaga
    31561 cggtgaattt tacgggatgg tgtttcagga cagcgacgtc gccaaatggc tggaagcggt
    31621 agcctggtcg ctgtgccaga agccggatgc cgaactggaa aaaaccgctg acgaggtgat
    31681 tgaactgatc gcctccgccc agtgcgaaga tggctatctc aatgcttact ttacggtaaa
    31741 agcgcccgaa gaacgctgga gcaatctggc ggagtgtcat gaactttact gcgccggtca
    31801 tctgattgaa gccggagtcg ccttcttcca ggccacggga aaacggcgct tgctggaagt
    31861 ggtttgccgt ctggccgatc atatcgacag cgtatttggt ccaggtgaaa gtaagttaca
    31921 cggttatcct ggtcacccgg aaattgaact ggcactaatg cgcctgtatg aagtgaccga
    31981 agagccgcgc tacctggcgc tgacgaacta ttttgtcgaa cagcgtggtg cgcaaccgca
    32041 ctattacgac caggaatatg aaaagcgcgg gcagacctcg cactggcaca cctacggccc
    32101 ggcgtggatg gtgaaagata aagcctacag ccaggcgcat ttgcccatcg cacagcaaca
    32161 aaccgccatc ggacatacag tacgctttgt atatctgatg actggcgtcg cacatctcgc
    32221 gcgtttaagt cacgatgaaa gcaagcgtca ggattgcctg cggctgtgga acaatatggc
    32281 ccagcgtcag ttatatatta ccggcggcat tggctcacaa agcagcggcg aagcgttcag
    32341 cagcgattac gatctgccga atgacacggt ttacgccgaa agttgtgctt ccatcggcct
    32401 gatgatgttc gcccggcgaa tgctggaaat ggaaggcgac agtcaatatg ccgatgtgat
    32461 ggagcgcgca ctgtacaaca ctgtgctcgg cggcatggcg ctggatggca aacatttctt
    32521 ctacgttaat ccgctggaag tgcatccaaa aacgttaaaa ttcaaccata tctacgatca
    32581 cgttaagccg atccgccagc gttggtttgg ttgcgcttgt tgtccgccaa atatcgcccg
    32641 cgtgctaacc tcgattggtc attatctcta cacgccgcgt gaagatgcgt tgtatatcaa
    32701 catctacgca ggaaacagca tggaagtgcc ggtagaaaat ggcacgctgc gtctgcgggt
    32761 tagcggaaac tatccgtggc aggaacaggt gacaattgcg gttgaatcgc cccagccggt
    32821 gcgtcatacg ctggctttac gtctgccgga ctggtgcaca cagccgcaga tcatattgaa
    32881 tggggaagag gtcgagcagg atattcgtaa agggtatttg cacattaccc gcgaatggca
    32941 ggagggcgac acgctgaatc tgactttgcc aatgccggta cgtcgcgttt acggtaaccc
    33001 tctggtgcgt cacgtcgccg gaaaagtggc gattcagcgc ggcccgctgg tgtattgcct
    33061 ggaacaggcc gacaacggcg agtcactgca taatctgtgg ctgcccgccg atgcgccatt
    33121 tacgacattt gaaggtaagg gattgttccg ccataagatc ttaatccagg caccgggtta
    33181 tcggtatgaa cagagcaatc cagagcagca accgctgtgg cattacgaca gcgcgccagc
    33241 caaacgccag ccgcaaaccc tgacctttat cccgtggttt agctgggcca accggggtga
    33301 aggcgaaatg cggatctggg tgaatgagga aaagcattgc catccggagg ttggataagg
    33361 cgctcgcgcc gcatccaacg tagggagcac attgcctgat gcgacgctta acgtgtctta
    33421 tcaggcccac gagtcggtgc gagagcgtag gccagccgca ccgacatcca ccggtaaatt
    33481 aaaaacgcca gctcacgccg gtgacaaagg taaaactctc attacgatca atcatcgggc
    33541 tgtcggtaat ctcttccggc agctcgctgt atccggcgct cgccatcaat acgacgtgct
    33601 ctcctatcgg gtattttgcc gtcaggctga cataaggcac ccaggcatcc tgcgcggaat
    33661 aacttgccag accgctacga cgggactcac tctctgaaat gccatagtaa tagtcactga
    33721 aattctcgtc ataatagaga acgcccagcg caggtgtcag cgacaggcga cctatctgca
    33781 ttttgtggaa taccgatagc tcccccaccc agccgttgct gttatccaga acgtccgcag
    33841 ccgcagaggc tttcacggtt ccccaacgct cgtggtgata ccaggcaacc cccgccatag
    33901 ccgtactatc acgcttatca agctgttgca tggcataatc gtcattatcg gtagggtcaa
    33961 attccagcgg catccaggat gcggtcaggc taagttcatt tttttcactt tgcgacagaa
    34021 taaaacctaa cgtggtctga cgaacataaa aagtatcacc ttcataacta atcagcggaa
    34081 ttgcctttgt attttcatta taaccgcgat aaggcgattc attataagcc gcaccagcac
    34141 caatggataa ttcagaagca gttgcgcttg ccataaaagg caacgcaaat aacgccacaa
    34201 tattgcggtt aattaacatg tccggtattc cattttaaaa taagataaaa aagagtcggc
    34261 atagtaatgc ttacaatttc tccggtgcaa gattcatatt tatatagtaa tatataaaat
    34321 tatatataat tgggctgttg cgatgacaaa attacaactt aagtaccggg agttaaaaat
    34381 tatctcggta atcgctgcca gtgaaaatat cagccatgcc gcgactgtac ttggcatcgc
    34441 acaggccaac gtcagcaaat atcttgctga ttttgaatca aaagtgggtt taaaagtctt
    34501 tgaccgaacc acccgacagc tgatgctcac accttttggc accgtgctgt taccgtatat
    34561 caacgacatg ctggacagaa acgagcagct taataatttt attgcagatt ataagcatga
    34621 gaaacgtggt cgggtcacta tctatgcacc aaccggcata attacctatt tatccaaaca
    34681 tgtaatcgat aaaattaaag atatcggtga tatcacctta tcgctaaaaa cctgcaatct
    34741 tgagcgtaat gccttttatg aaggcgtcga atttcccgat gattgtgatg ttttaatcag
    34801 ctacgcccaa ccaaaagatg aatcgctggt tgccagtttt attacccaat atgccgtaac
    34861 cgcttatgcc agccagcgct atcttgagaa acatcccatt agccgtcctg acgaactcga
    34921 acatcactcc tgtattttga tcgactcgat gatgatcgac gacgcgaata tctggcgctt
    34981 caacgtcgcc gggagtaaag aggtgcggga ttatcgcgtg aagggaaatt acgtttgcga
    35041 caacacgcaa tctgcgctgg agctggcacg aaatcacctg gggattgtgt ttgcgccaga
    35101 caaaagtgtg cagagcgacc tgcaagacgg cacgctggtg ccctgctttc agcacccgta
    35161 tgaatggtgg ctggatctgg tggctatctt ccgtaaacgg gaataccagc cctggcgggt
    35221 ccagtatgtg ctggatgaaa tgctgcgtga aattcgccac cagcttgctc agtcgcagca
    35281 actgcggcct gaacaagcgg cggaaagcga agattaatga tcgagataaa ggtaatgcac
    35341 ccagctggtc atgcgtaaca gtactttgcg catcaccgag acatggctga aatgatcaga
    35401 atagaccgcc acctgggcgt aaataccgtc tggtaatgcg ctcaaatcag tatgctcatc
    35461 cagttcaatg gtcgcgataa cgccatctga acccggcgct gtgtttaacg tctgtaaggt
    35521 gccggtcgac tgataagctc caccgggaac agctggacta atggctgcca gcttaccgct
    35581 gaacactttg cctggcagag cgttaaatac cacttccgca tcatcgccgg gagccaggcg
    35641 cagcaaggag ttctgacgga actgcgccac gatttgtcgt ttctgatcgg gtataaacac
    35701 catcaccgga cgtagcggca gcgacgcggc ataggtaccc ggacgaatca gcacctgggt
    35761 cacataacca tcgctcggcg cacgcactat cgtctgctca aggttatatt ttgcttccgc
    35821 gagctgcgct ttcaggctgg cgatttgaga atgttcaccc aacaccaggc tatccagctg
    35881 gctctggatc tgtttttgtt ccgccgccgc tgattttacg gaggcttcct gcgccagata
    35941 attttgccgc gccacatcga tatcgcgttc tgaaaacggg tttactttcg cctggctgcc
    36001 acgtgcgtaa cgctgatact ctttagcgaa cttatcccgc gtggcctttg cctgctgagt
    36061 attcgccgcc atctcatcta actctgcgcc caacgcccgc tgtttatgtt ctgcggtaac
    36121 gatatccgcc atcagccgat ccacccgagc ctgataacgc gtcgggtcca gtcgaaatag
    36181 cacctcacct tttttaatca gcgtattttt cttgtccgtc acttcgatca ccacgcctgt
    36241 cacctgtggg acaacaggaa tagaaataac cgctttttgc gctttaaagg tatacggatg
    36301 gttatagttc attaacagaa ttagaccgct gacaataaat atgcctccca gggccgctgt
    36361 gggaatggtc catttattta caggaatttt gaagatctta aacattgccc atgcgaaagc
    36421 cacataggtt aaaataatca gtaaatccat aagtattact cggcagaaga ggatttgatg
    36481 tcagcgagct gtttctccag cccggcaata cgctgttgta gttgcacaac ggaggagtca
    36541 tggttttgca ttccccatcc acgctccggg cggtaaagcg tggcccaaat ccacaaaaat
    36601 ggccagataa catgtaacgt aaaaagactc acccaaccag caacatgaat ggcgtcggca
    36661 tgaggatgat tacgtttttt ggcaatcagg tagggaacat catgtaaaat aataatccca
    36721 tagaatatca ccagaaatac aaaaataagc actcccaatg caaaataatc caggaacata
    36781 tttccctcag agaatataga aaagaacaat taacgcatat tatgaaatac cataatgcgg
    36841 tgcaatttcc ttatttatat aacgacatat tgttttttat atactttctg aagtgtgcag
    36901 cctggctttt caacagtgcg aggcttatca tattgaaata atggaactat tggccaaact
    36961 aatgaataac tccagtaaaa catccacagt acagattaag cgtattaaac cttcaattat
    37021 ctaccgttta ttgctgattg gcctcggaac gccaatggtg atttatggcc tggttcgccc
    37081 gctcaccatc gaaacgcgag attaaactcc tggcaaaaca cgccccagaa acgcacttct
    37141 gttgacgtag tctgggttat tgcgcacgta ggtttaaaac gtaaattgta acaacgtgcg
    37201 ctttgtttat gccggatgcg gcgtgtacgc cttatccggc ctacggtcag gtccccgtag
    37261 gcatgataag acgcgtaagc gtcgcatcag gcaatgaata cccaatgcga ccagcttctt
    37321 atatcagaac agccccaacg gtttatccga gtagctcacc agcaggcact tggtttgctg
    37381 gtaatgctca agcatcatct tgtgggtttc gcgtccgatg cccgactgtt tgtagccacc
    37441 aaacgccgca tgtgccgggt aagcgtgata acaattggtc cacacgcgcc ctgcctgtat
    37501 gccgcgcccc atcttatagg caagattacc gttgcggctc cagacgcccg cgcccaggcc
    37561 atattgcgta tcgttcgcca gctccagcgc ttcttccatc gttttgaagg tggtcaccgc
    37621 cagcaccggg ccaaaaatct cctcctggaa cacgcgcata ttgttctgac caaacaaaat
    37681 cgtcggttcg aggtagtagc cgtctttcag ttcaccttcc agcagcttgc gccgcccgcc
    37741 agtgagcacg tctgcgccct cttttttacc gatatcaatg tagttgagga tggtttccag
    37801 ttgcccgtga gaaacctgcg cccccatttg cgtcacgctg tcgagcgggt taccgctgcg
    37861 aatgctttcg acacggcgga tggcgcgttc cataaagcgt tcgtagatag actcctgcac
    37921 taaagcacgg cttggacagg tgcaaacttc gccctggtta aaggcaaaca gtgcaaagcc
    37981 ttccagcgct ttatcgaaaa aggcatcttc ttcatccatt acgtcagcaa agaagatatt
    38041 tggcgatttg ccacccagct ccagcgtcac cggaataatg ttttgcgtgg cgtattgcat
    38101 aatttgttgg cccacttccg ttgagccggt aaacgccact ttggcgatgc gtttcgaggt
    38161 cgccagatat tcgccaattt ccccacctgc gccgttgacc acgttcacca cgcccggcgg
    38221 cagtaaatca ccgacgattt ccattagcag cagtacagaa agcggggtaa gacgtgcggg
    38281 tttcagcacc acacagttgc ccgccgccag cgcgggagcc attttccagc tcgccatcag
    38341 cagcgggaag ttccacggaa taatctgccc caccacgcct aacggctcat ggaaatgata
    38401 ggccacggtt tcgctatcaa cttcactgat cccaccttcc tgcgcccgaa tacacgaggc
    38461 aaaatagcga aaatgatcaa tcgccagcgg cacatcagca gcactggttt cgcgaatggg
    38521 tttgccgtta tcccaggttt cagcggtcgc taacagttca aggttttgtt ccattcgatc
    38581 ggcaatctta aacagaatcg ccgcacgatc ctgcaccgag gtgtgcgccc atttatcttt
    38641 cactttgtgc gcagcatcca gcgccagatc gatgtctcgt ttgcccgaag acgccacttc
    38701 gcacagcagc tgcccggtca ccggcgtcag gttctggtaa tactcgccat cggcaggcgc
    38761 tacccattcg ccaccgataa agttgtcgta gcgggctttt aatttgaggg ggaaaccata
    38821 ctcgccgggc ttaatctgtg ctgaaggggg attattggtc atgatcgtct ccttgcggtg
    38881 tgaggtataa caagggtaga cgtgactggc gaaatcttcg ccagtcggta acagctttac
    38941 gacggctatc acgaatttac gggcaaaagc tacagcgggt agtgtgcgac gtacacggct
    39001 tgctggttat taattctttt ctcttgcgcg aatccttctt taacaattgt ccgtaaatca
    39061 gctcgagccg tattatcaga gacgcccaag ctcgcgctaa tttcagcaac tgtgtactct
    39121 ttccccggat tggcgagcat cacattcagg agcgttacct ggcgcggatt caggcgttta
    39181 agcacaccag agctaaacag cacactatcg agttttgcgc ttcgttgtgt aatttcatcg
    39241 atatgctgct gccagttttg cagagcacgt ttaatgacac ccgcctgata ttcaaggaaa
    39301 taagtcagat ccataccatc tgattcagtg tactggtagc tggcggcata tttgaccggt
    39361 gcagcatgaa gcaaacggct gatggaaata tatttaaaga catcgtagcc cgatttcagc
    39421 atataccagt aaaacagcgc ccgactggtg cgcccgttgc catcgcgaaa cggatgttca
    39481 tgggccagca taaaatgcag aatgcaggca cggaccaacg gatgaatata cccttcatgg
    39541 ctgtttaacc actggcaaac tttttccagc cgttccggta acaatgccgc agcgggcggt
    39601 tgatgcacta tattgccatc atagtcggcg atcacaacat catctgtttc acgaaattct
    39661 cccggacgat atttcgcgtc attgattcca cccataccaa tggcatgtaa ctggcgaata
    39721 agcgcaggtg tcagcggttc tgcgagtaag tgcggaattt ccgccatcag ccgggcattt
    39781 cccgcaatca tatgctcgtc ttcagtgcgt ggcgaccgcc ctgtttccag catttttctc
    39841 gccaccagcg ttgtagtatt cgcaccttcc agttgggaac tggtgatcgg ctcttccagg
    39901 cgtaactgac ttaactcggc gcccgccccg cgtaaccgtt cagtctgatc tttcagcgcc
    39961 agtgcagtgg catgtttatc aacaagctcg cacgcttctg caatcaccgg cgtaatgttg
    40021 aatccggcct gttcacccgc ttcattgcaa taattaattc gttggatcgc cgcgttgcgc
    40081 gccagacggg taagcgtcca cgccacatcg acgttttctc cttttttgat gcggtactgc
    40141 agctcatcaa aaggcagata acgtcctttt tcatcggtaa cccggttaaa gcgcaggtgg
    40201 gccaggagtt cctcacccga taagtgtgat aaaggaataa gtccgggagg tgatttgata
    40261 gccatgtgct tatctcaaaa aagcgcaata ttgagatagt gtatagcagg aaagagaaac
    40321 tatctcaaaa atcgcaaatt ttgagatagt ttaaaaaatg gctgacaatt agacagccat
    40381 tgtgggaatg atgattacat cgctgcgcga taaatcgcca taatttcttc gtgagttgcc
    40441 tgaatcgggt tagtaaaccc acacgcatct ttcagggcat tggttgccag cactggaata
    40501 tcttcttctt tcacgttcag gtcacgcagg cctgccggga tattcacttt cttcgccagt
    40561 tcacggatgg cattgatgca cgcttctgcg ccttccgcgt catttttgcc tgtcacgtta
    40621 acgcccatgg cagcagcaca gtcacgcaga cgcgcagcag cgactttgct gttgaacacc
    40681 tgaacgtgtg gcagcagaac ggcattacat acaccatgtg gcaggttgta gaaaccgccc
    40741 agttggtgcg ccatcgcatg aacataacca agagatgcgt tgttgaatgc catgccagcg
    40801 aggaactggg cataagccat tgcttcacgc gctttagcat tgctgccatc ttcaacggct
    40861 aacggcaggt tttcggcaat catggtcacg gctttcagtg cgcaagcgtc agtgatcggc
    40921 gtggcggcaa tagaaacata cgcttcgata gcgtgcgtta aggcatccat acccgttgcg
    40981 gcggtcagtg acttcggcat accgaccatc agagatgagt cattgacaga aagcaacgga
    41041 gttacatgtt tatcaacaat cgccattttg atatgacgcg cttcgtcagt gatgatgcag
    41101 aaacgggtca tttcagaggc cgtacccgcc gtggtattga tggcgatcat cggcagctgc
    41161 ggttttgcag agcggtcaac gccttcgtaa tcgcgaatat cgccgccatt ggctgccacc
    41221 agcgcaatac cttttgcgca gtcgtgcgga gaaccaccgc ctaaggagat cacgctatcg
    41281 caattatttt ctttaagtaa ttttaaacct tcggcgacgt tttccgtggt ggggttaggt
    41341 tgggtgccat cataaataac gctaaaaata ttgcgttctt ccagtgcttt ttgcacatca
    41401 cccgccatac ccaatttcgt taacatgttg tcggtgacaa ttaaggtacg ggtaaatcca
    41461 taatctgcca tcatattcat tgcatcattc aatgaatcag caccaataac attcacggaa
    41521 ggaataaaaa aggttgaagc tgccataaca cactcctgaa agtgtaaaaa agggcgggca
    41581 gcttactgag gattttcatt tgaaaatatg atcaacatta ccgtttatta aaatattttt
    41641 aataaaaagc acgttacatt catcaaataa cgtgccttct taacattaat aagaaatttt
    41701 gtattaattc atatttatta tgaattaatc atttccttat ttttccggaa ataataatgc
    41761 gtcgcgcaat aaatgatcat ttccacgacg acgcgtaaag ccaatgcggt caaaatattc
    41821 caggatttga attgccagct ttcggcctac gcctaagcga tcgcggaaat ccgccgcgca
    41881 ggttgaacca cattcctgat cgagatcgcg gatcatattg gcaaattcga caatccgatc
    41941 gttacggtaa taacgatctt tgacgatcgc agtaattatc ccctgttgcg ccgcctggcg
    42001 tagcgtcagg cgcattgcct gctcgtcggt tcccgtttct tttgccaggt ctcgcaccca
    42061 ccacggttcg tcaccaaaca gtggctctgc tttttgccag atggcctgct gctcttcgct
    42121 gaagcccgct ttgtgatccg gcagatgcag ccagccgtga tgactgagga tgtcgccgct
    42181 ttcgcgcatc ttttcaatca gcaacagtac cagcgcttca tcttccattg gcaacgccat
    42241 acgtcgcaga cgttctcgcc caggaccagg ttcatcgcga tgttgctcat gataagtcgc
    42301 taatgtgtcg agaattttcc gctgccagcg agcggcaacc ggcgcattca acaagctata
    42361 accagcctga atatagccag gctgttgcag cagttcgcgc atcccttcgc cgttgagctg
    42421 ccgtgcccag gcgaaatccg caaggttaac cgctccgcgt tccagatgaa cagataacgc
    42481 atcggcatcg ctctgcgccc gtgcaagaga cgccagccat tgcagatatt ccggcttacg
    42541 cttaccgcga cgcggcgggt taagcatcac cacacgcgcc cccgccagcg tgttgcgggc
    42601 cgagatatcg cgcaacacta agcgatcgtt atccgccagc cacagcggcg tgtcgaacac
    42661 cagctcggca aggttatctt ccagcagcga aacgcgtccc gtgacgtggc tggcggcgtg
    42721 gtgaatatgc agcggctgcc actgggtcag cggtgtatgg gtttgaagct cgacaatcac
    42781 ccgtgtgaac ggctccggcg gcgcatcggc aagcagccag tcgccacggt taatctgctc
    42841 tttttccgca tcacccgcga tgttaagcgc gatacgctgc ccggcatggg cagtttctgt
    42901 tggctgattt tgcgcatgca gcgcacgcac gcgcatcggt ttatttacac cagtcagcca
    42961 gagtgaatcg cctaccttca cttccccgct taacgccgta ccggtgacga ccagcccggc
    43021 accttttacg gtaaatgcac ggtcgatcgc gaggcggaaa ctatgttggc tggcgtgttc
    43081 gcgttctggt aactgaagca gatgctcgcg cagggcatca attccccgac cttcggttgc
    43141 tgcggtgata aacagttttg cctcagcaaa accgtattcc cgcagcacct cctttacctg
    43201 gcgttcaacc tcatcaacac gcgcttcgtc cacgcgatcg gctttggtca gcgccactgt
    43261 cagcatcggg ttaccagtca gctgcaaaat cgccagatgc tcacgggtct gtgccatcac
    43321 accgtcatcg cacgccacca ccaacagcgc gtgatcgata ccaccgacgc ccgccagcat
    43381 gttggaaaga aacttttcat gaccgggaac gtcgataaaa ccaggcacgc ggccatccgg
    43441 ctgcggccag taggcatagc cgagatcgat agtcatgccg cgcttttttt cttccggcag
    43501 acgatcggca tttacgccag taatcgcctg caataaggtt gttttgccgt ggtcaacgtg
    43561 tccggcagtc gcaataatca tttcaacaac atctccaaaa accgttgctc atcttcaagg
    43621 cagcgtaaat ccagccacaa tcgtccgtca taaatacgac caatcaccgg cactggcaat
    43681 tcacgccagc gggcggctaa tgactcaagg tggctaccgc gtccatcatg gggtgtaaac
    43741 gttaatgccg cgctcggcag gcgatcaacc ggcagtgaac cactgccaat ctgcgaaaga
    43801 catggcataa cctggactgc aaactccgcg ccgtaatgtg cggcaagggg agcctgtaaa
    43861 cgttgtgcct ggatttgaat gacctctgcg ctgcgggtaa gcaggcgcag ggtcggtaat
    43921 ttttcactca gagcttcagg gtgtaaataa agacgcaacg tggcttccag cgccgcgagg
    43981 gtcattttat ccgcgcgtaa tgcacgcttc agcgggtggc tttgcaggcg ggcgatcatc
    44041 tcttttttac caacaataat tcctgcctgc ggcccgccta acaacttgtc accggagaaa
    44101 ctcaccagac tgacgcccgc cgcaatcaac tcctgcggca ttggctcttt cggcaaaccg
    44161 tactggctaa gatcaaccag cgagccactg cctaaatcag tcactacggg aacatccagc
    44221 tctttgccga gcgccaccag ttccgcttca tctatcgctt tggtgaatcc ctgaatgctg
    44281 tagttactgg tatgtacttt catcaacagc gcggtatttt cattcaccgc ctgacgataa
    44341 tcattagcgt gcgtgcggtt ggtggtcccc acttcgtgta gggtgcagcc tgcctgacgc
    44401 atgacatcag gaatacgaaa cgcgccgcca atctccacca gttcgccgcg agataccacc
    44461 acctctttcc cgctggcagt ggccgccaac atcaataaca ccgccgccgc attgttatta
    44521 acgatacagg catcttccgc tcccgtaatg cggcacagca gctgcgccag cgcccgatcg
    44581 cgatgtccgc gtccggcgtc gtccagatcg tactcgaggg tcactggcga acgcatagcc
    44641 tgcgcaacgg cttccaccgc ggcttctgcc tgtaaagctc gcccaagatt ggtatgcagc
    44701 acggttcccg tcaggttgat caccggacgc agcgcgctct gcgcctcttt cgtcaaccgg
    44761 gcatcgactt cttgcgccca gttttcacac catgcgggca gcgtctggct atcacgaatc
    44821 acttctcgcg cttcgtcaag catctgacgc agcaattcca ccacgcgggt gtgaccataa
    44881 gtatcacgca aagaaaggaa ggagctatcg cgcaataagc gatcaatagc cggaagttga
    44941 ctatagaggg aacgcgtttc ggttgtcata ggaaacctgg ctgatcaagg ccctctcaca
    45001 cggagaaggg cgtttaacat aaccacggat tgtaacgtga gatgggtcag gaggacatat
    45061 cgcgcatcaa gcctttggcg gttcggtgcg agcaaaactt tcgcggctaa acaggttttc
    45121 gaccagtttg actaagtgtg ggcggtcgac acaccagcct ggcgcaacgc ggcggaaatt
    45181 gagatatccg acggcacagg caatggcgat agtcgccaga ttgaccgtat cggttttgag
    45241 cgtgccatcg accagatatc cttccagcac atccagactg cggttgattt tctcgcgctg
    45301 gcgtaacaat tcatcttcag actgctgcgc cgctggacgc gcctgttcac gcaccgatac
    45361 cagcccggca tccataatgc catccgccag tgcctcaatt ttgcgcaccc gcaacgactc
    45421 cagcggatcg cgcggcaaca tcgccggagc gacattcatt aattcaatat attcagcgat
    45481 gatcggcgaa tcaaaccagc attcgccctc ttcggtcacc agcaccggca ctttccctaa
    45541 cgggttaaat tgcgccacgc cgttatccgc gttatagggc agttcattaa taaattcgaa
    45601 agttatgccc ttttctaaca acagaataga aagtttgcgt acaaacgggc tggtgtagct
    45661 accgacgagt ttcatgccga gtcctttgtg cgaggaaaaa tatcagtatg gcctggtgat
    45721 aggaaaaagg
//
LOCUS       DAZKGF010000017        45501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZKGF010000017 DAZKGF010000000
VERSION     DAZKGF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.017"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(30..2078)
                     /gene="prc"
                     /locus_tag="ACHOQV_000928"
     CDS             complement(30..2078)
                     /gene="prc"
                     /locus_tag="ACHOQV_000928"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFP9629342.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQTFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(2098..2796)
                     /gene="proQ"
                     /locus_tag="ACHOQV_000929"
     CDS             complement(2098..2796)
                     /gene="proQ"
                     /locus_tag="ACHOQV_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFP9629343.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(2893..3390)
                     /gene="msrC"
                     /locus_tag="ACHOQV_000930"
     CDS             complement(2893..3390)
                     /gene="msrC"
                     /locus_tag="ACHOQV_000930"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFP9629344.1"
                     /translation="MNKIEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHVFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            3520..4803
                     /gene="yebS"
                     /locus_tag="ACHOQV_000931"
     CDS             3520..4803
                     /gene="yebS"
                     /locus_tag="ACHOQV_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFP9629345.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPTTRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            4772..7405
                     /gene="yebT"
                     /locus_tag="ACHOQV_000932"
     CDS             4772..7405
                     /gene="yebT"
                     /locus_tag="ACHOQV_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFP9629346.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            7485..8924
                     /gene="rsmF"
                     /locus_tag="ACHOQV_000933"
     CDS             7485..8924
                     /gene="rsmF"
                     /locus_tag="ACHOQV_000933"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFP9629347.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDSEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            9042..9278
                     /gene="yebV"
                     /locus_tag="ACHOQV_000934"
     CDS             9042..9278
                     /gene="yebV"
                     /locus_tag="ACHOQV_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFP9629348.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            9383..9574
                     /gene="yebW"
                     /locus_tag="ACHOQV_000935"
     CDS             9383..9574
                     /gene="yebW"
                     /locus_tag="ACHOQV_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFP9629349.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(9575..10231)
                     /gene="pphA"
                     /locus_tag="ACHOQV_000936"
     CDS             complement(9575..10231)
                     /gene="pphA"
                     /locus_tag="ACHOQV_000936"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP9629350.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWFMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGIKGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(10626..10967)
                     /gene="yebY"
                     /locus_tag="ACHOQV_000937"
     CDS             complement(10626..10967)
                     /gene="yebY"
                     /locus_tag="ACHOQV_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFP9629351.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(10980..11852)
                     /gene="copD"
                     /locus_tag="ACHOQV_000938"
     CDS             complement(10980..11852)
                     /gene="copD"
                     /locus_tag="ACHOQV_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFP9629352.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWLAVLQTQFGGIWLWQIVLAL
                     VTLIVALMQPQNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLMLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(11856..12230)
                     /gene="yobA"
                     /locus_tag="ACHOQV_000939"
     CDS             complement(11856..12230)
                     /gene="yobA"
                     /locus_tag="ACHOQV_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFP9629353.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            12369..12599
                     /gene="holE"
                     /locus_tag="ACHOQV_000940"
     CDS             12369..12599
                     /gene="holE"
                     /locus_tag="ACHOQV_000940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFP9629354.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            12701..13357
                     /gene="yobB"
                     /locus_tag="ACHOQV_000941"
     CDS             12701..13357
                     /gene="yobB"
                     /locus_tag="ACHOQV_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFP9629355.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVSAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            13381..14043
                     /gene="exoX"
                     /locus_tag="ACHOQV_000942"
     CDS             13381..14043
                     /gene="exoX"
                     /locus_tag="ACHOQV_000942"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFP9629356.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(14040..16100)
                     /gene="ptrB"
                     /locus_tag="ACHOQV_000943"
     CDS             complement(14040..16100)
                     /gene="ptrB"
                     /locus_tag="ACHOQV_000943"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFP9629357.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPED"
     gene            complement(16309..16968)
                     /gene="yebE"
                     /locus_tag="ACHOQV_000944"
     CDS             complement(16309..16968)
                     /gene="yebE"
                     /locus_tag="ACHOQV_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP9629358.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(17295..17651)
                     /gene="yebF"
                     /locus_tag="ACHOQV_000945"
     CDS             complement(17295..17651)
                     /gene="yebF"
                     /locus_tag="ACHOQV_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFP9629359.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQPDPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(17718..18008)
                     /gene="yebG"
                     /locus_tag="ACHOQV_000946"
     CDS             complement(17718..18008)
                     /gene="yebG"
                     /locus_tag="ACHOQV_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFP9629360.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            18142..19320
                     /gene="purT"
                     /locus_tag="ACHOQV_000947"
     CDS             18142..19320
                     /gene="purT"
                     /locus_tag="ACHOQV_000947"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFP9629361.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADNENLFREAVAEIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSQLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGIQIRLFGKPEIDGSRRLGVALATAESVDEAIERAKHAAGQVKVQG
                     "
     gene            complement(19376..20017)
                     /gene="kdgA"
                     /locus_tag="ACHOQV_000948"
                     /gene_synonym="eda"
     CDS             complement(19376..20017)
                     /gene="kdgA"
                     /locus_tag="ACHOQV_000948"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9629362.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(20054..21865)
                     /gene="edd"
                     /locus_tag="ACHOQV_000949"
     CDS             complement(20054..21865)
                     /gene="edd"
                     /locus_tag="ACHOQV_000949"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFP9629363.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(22100..23575)
                     /gene="zwf"
                     /locus_tag="ACHOQV_000950"
     CDS             complement(22100..23575)
                     /gene="zwf"
                     /locus_tag="ACHOQV_000950"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP9629364.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            23913..24782
                     /gene="hexR"
                     /locus_tag="ACHOQV_000951"
                     /gene_synonym="yebK"
     CDS             23913..24782
                     /gene="hexR"
                     /locus_tag="ACHOQV_000951"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFP9629365.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAIVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            24910..26352
                     /gene="pyk"
                     /locus_tag="ACHOQV_000952"
     CDS             24910..26352
                     /gene="pyk"
                     /locus_tag="ACHOQV_000952"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP9629366.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(26484..27455)
                     /gene="lpxM"
                     /locus_tag="ACHOQV_000953"
                     /gene_synonym="msbB"
     CDS             complement(26484..27455)
                     /gene="lpxM"
                     /locus_tag="ACHOQV_000953"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP9629367.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(27575..28897)
                     /gene="mepM"
                     /locus_tag="ACHOQV_000954"
     CDS             complement(27575..28897)
                     /gene="mepM"
                     /locus_tag="ACHOQV_000954"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFP9629368.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(28913..29845)
                     /gene="znuA"
                     /locus_tag="ACHOQV_000955"
     CDS             complement(28913..29845)
                     /gene="znuA"
                     /locus_tag="ACHOQV_000955"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFP9629369.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            29924..30679
                     /gene="znuC"
                     /locus_tag="ACHOQV_000956"
     CDS             29924..30679
                     /gene="znuC"
                     /locus_tag="ACHOQV_000956"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFP9629370.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRHELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            30676..31461
                     /gene="znuB"
                     /locus_tag="ACHOQV_000957"
     CDS             30676..31461
                     /gene="znuB"
                     /locus_tag="ACHOQV_000957"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFP9629371.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAIMFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(31611..32621)
                     /gene="ruvB"
                     /locus_tag="ACHOQV_000958"
     CDS             complement(31611..32621)
                     /gene="ruvB"
                     /locus_tag="ACHOQV_000958"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP9629372.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPE
                     MP"
     gene            complement(32630..33241)
                     /gene="ruvA"
                     /locus_tag="ACHOQV_000959"
     CDS             complement(32630..33241)
                     /gene="ruvA"
                     /locus_tag="ACHOQV_000959"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP9629373.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(33380..33445)
                     /gene="yobI"
                     /locus_tag="ACHOQV_000960"
     CDS             complement(33380..33445)
                     /gene="yobI"
                     /locus_tag="ACHOQV_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFP9629374.1"
                     /translation="MCIFITHFFAEYVIFKYLLPI"
     gene            33516..34118
                     /gene="yebB"
                     /locus_tag="ACHOQV_000961"
     CDS             33516..34118
                     /gene="yebB"
                     /locus_tag="ACHOQV_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFP9629375.1"
                     /translation="MNVNYPAEYEIGDIVFTCIGATLFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(34120..34641)
                     /gene="ruvC"
                     /locus_tag="ACHOQV_000962"
     CDS             complement(34120..34641)
                     /gene="ruvC"
                     /locus_tag="ACHOQV_000962"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFP9629376.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(34676..35416)
                     /gene="yebC"
                     /locus_tag="ACHOQV_000963"
     CDS             complement(34676..35416)
                     /gene="yebC"
                     /locus_tag="ACHOQV_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9629377.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(35445..35897)
                     /gene="nudB"
                     /locus_tag="ACHOQV_000964"
     CDS             complement(35445..35897)
                     /gene="nudB"
                     /locus_tag="ACHOQV_000964"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFP9629378.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(36015..37787)
                     /gene="aspS"
                     /locus_tag="ACHOQV_000965"
     CDS             complement(36015..37787)
                     /gene="aspS"
                     /locus_tag="ACHOQV_000965"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP9629379.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKDENN"
     gene            38097..38663
                     /gene="yecD"
                     /locus_tag="ACHOQV_000966"
     CDS             38097..38663
                     /gene="yecD"
                     /locus_tag="ACHOQV_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9629380.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            38660..39478
                     /gene="yecE"
                     /locus_tag="ACHOQV_000967"
     CDS             38660..39478
                     /gene="yecE"
                     /locus_tag="ACHOQV_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP9629381.1"
                     /translation="MIYIGLPQWSHPKWVRLGITNLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            39531..39926
                     /gene="yecN"
                     /locus_tag="ACHOQV_000968"
     CDS             39531..39926
                     /gene="yecN"
                     /locus_tag="ACHOQV_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFP9629382.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            39967..40710
                     /gene="cmoA"
                     /locus_tag="ACHOQV_000969"
     CDS             39967..40710
                     /gene="cmoA"
                     /locus_tag="ACHOQV_000969"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFP9629383.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            40707..41678
                     /gene="cmoB"
                     /locus_tag="ACHOQV_000970"
     CDS             40707..41678
                     /gene="cmoB"
                     /locus_tag="ACHOQV_000970"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFP9629384.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(41843..44272)
                     /gene="torZ"
                     /locus_tag="ACHOQV_000971"
     CDS             complement(41843..44272)
                     /gene="torZ"
                     /locus_tag="ACHOQV_000971"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFP9629385.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPICSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGSADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(44297..45397)
                     /gene="torY"
                     /locus_tag="ACHOQV_000972"
     CDS             complement(44297..45397)
                     /gene="torY"
                     /locus_tag="ACHOQV_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFP9629386.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGVLTDPALADRKPLWQYAEKLDDTYCAGCHAPIASDHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT        10776 a        11809 c        11271 g        11645 t
ORIGIN      
        1 caggcacaat ttcttgtgcc tgattgatat tacttgacgg gagcgggttg ttccgcgggt
       61 ctggcttttt caagcttcgc cagatcgagt gcgatattca ccgtctcatc cagataagga
      121 tccggctcct ggtaatcttt cggtagatca tccagtttct tcaactccgg tttaccttcg
      181 cgtttaaagc gttcgttcaa acgcgccaga cgcgtcgcat catcttcatt attctctttc
      241 tcacgcacag cgtaattcag agaaacgata ttgcgcttgt ccttcatggc gttgaagcgc
      301 gcgatatcct tcatgatgtt ctggaactca ggatctttcg cgatacgcgc attatgttcc
      361 ttcagcagct ccggtccaaa ggccgttaaa tcgcctgatt tcacataggt cgcggcatcg
      421 atgctatccc acggcagcgc gttatcttcg aatttctcac ccgtttccgt ttcttcatta
      481 cccgtcggca tgatgatgtc tggcgttaca cctttacgtt gcgtactgcc gccgttaacg
      541 cgatagaatt tctggatcgt gtactgtaca gatcccagcg ctggccattc aggacgtaac
      601 atctgatcgt agatacggtt caatgaacgg tattgctgaa cggtgccttt accaaacgtc
      661 ggttcaccca caaccagcgc acgaccgtaa tcctgcattg ccgcggcaaa gatttctgaa
      721 gccgaagcac tgaaacggtc aaccagtacc accagcgggc ctttatagaa gacctgtccg
      781 tcggtatcgc tgtcttcacg aactttaccg ttgttatcac ggacctgaac aatcgggccg
      841 gatggaataa acagaccgga gagcgatacc gcttcggtca gcgccccacc accattgcta
      901 cgcaggtcga tgatgacgct gctgacattc tgtttttcca gtttctgcag ttgcactttg
      961 acatcgtctg tcaaacccac atagaagccc ggaatatcca gcacgccgac tttctcttta
     1021 ccgacggtct tcaccgacat tttaaccgcg cggtcttcga ggcgaatacg ttcacgggtc
     1081 aacgttacag tacgggtctt ggtcccttta ccagcaggta aaatttccag acgaacttta
     1141 ctgcccttcg gccctttaat taaggcaacc acatcatcaa gacgccagcc aatcacgtca
     1201 accatcggct tgcctgtttg accaacaccg acaattttgt caccaacgct gatagcttta
     1261 ctcttcgctg ccgggcctcc tgccaccatc gaattgataa cggtgtagtc atcatccatt
     1321 tgcagcactg cgccaatacc ttccagcgac aaactcattt cagtgttgaa ctgttcggta
     1381 ttacgcgggg aaagatagtt ggtatgcggg tcgatttcac gcgcaaacgc cgtcattgcc
     1441 agcgagaaaa catcttcgct gttggtttgc gccagacgac gaatggcaaa tttgtagcgg
     1501 cgagtcaggg tttcacgaat ttctttatcc gtttttcctg ccagcttcag gcttaactcg
     1561 tcgaatttga ctttactgtc ccacagcgcg ttcaactcag cctcgttttt cggccagggc
     1621 gctttgctgc ggtcaaggtt ataagtgtcg ttgccggtga aatccatcgg cttttccagt
     1681 accgacaaag cgtactggta acgttcaaaa cgacgctttt gcgccagatt gtagagatcg
     1741 tagaaaacgt cgagtttgcc tgaacgcagt tcatcgccta actcggtttt ctttttcgcg
     1801 aactgttcaa catcgcttgc caacagcacg ttgtggctgt aatcgagcag attcaggtag
     1861 cggtcaaaga ttttggccga aaatgtctga tcgaggtcga actggcgata atgagaacgg
     1921 gtgaagcgcg acgttacgcg ctcacttacc gtcgcatgct gcgtctcttc ctttaatacc
     1981 ggaatttgat cagcacgcgt gatatcttct acagcgaagg tctggcctgc tattgcaagc
     2041 aggccagcta acgcggtaag cctaaaaaac atgttcatgc ctggcccggc ctccgtttca
     2101 gaacaccagg tgttctgcgc gcacaatcaa agacataccc gaattcagct ggacgcggac
     2161 gccgtctttg gtgatttcta atacggtggc atccatcgcg ttttgacccg ctttcacctt
     2221 cagggcttgt ccgacagtca gagctgaaat gtcagaaacc ggggtgtgct gttcttcgcg
     2281 aggtgctttt actgtttttg gcgctttctc taccggcttt tgcgcacgag gtttacgttc
     2341 agcgccttct ttgcggcgtg gcgtggtcgg acgtggcttg cgttcgcggc gcggtgcgtc
     2401 ttctttctca ccagcagctg cggcagcttc gcgttttttc gcttgctgtt cagcacgctg
     2461 tgcctgaaca cgcgctttcg cttcttcaag ctgcttgcga gcatgctcta catgttgctc
     2521 gtccagctca ccgcatgggt tgccgtcaag atcgacacgc gttgcgccgg gtttaacacc
     2581 gtaaagataa cgccagctcg aagtgtagag acgtaaagcg gatcgcaatt gcgttttgct
     2641 caggttcatt tccccggcaa cacgatcgac caaatcctga aaaataccga ttttcagcgg
     2701 acgcgcttca ccttccgcac tgaaacagtg gggaaaacgt tcggccagaa acgcgattac
     2761 ttctttactg ctattcaact taggttgatt ttccatgaaa tttcctgatt acaacggacg
     2821 tagccaacaa gcgcaggcat gaacaggcgt cattataatg acgctatcag taaatgctac
     2881 gttatccgtt gattatcctg cgacgctcgc aaagaatttt ttgtaatccg tcgttgcaag
     2941 cactttttca agctgtgcca caagctgacg taagccttgc tcgtcctcgt ctgtaaagcg
     3001 accgaagacg gtactatcga tgtcgagaac accaataatc tgatttttca ccaccagcgg
     3061 cagaacaatt tcagaattac tcgccgcatc acaggcaata tgcccgtcaa acacatgaac
     3121 atcctcgata cgctgcactt gattgcgggc aaccgcagtg ccgcacacgc cgcgcccgac
     3181 gggtatccgg acacaggcaa ttttgccctg aaatggtccg agtaccagtg tatcgtcctc
     3241 aagcaaataa aaacctgccc agtttacgtc agtgagacgc tcatataaca acgcactggt
     3301 gttcgcaagc gttgccagaa aactggtttc tcctgccatc agcgcgttaa agtcgcgatt
     3361 taaatccgcg taaaattcta ttttgttcat tatgtaatca cttggttgtc ttaccaggaa
     3421 ctgccagcct attaaaataa gcattaaatg cgttaatgct caagatcatt ccaatcatgg
     3481 gttaagatta atgttaattc tttttacatt tggcacgtca tggctcttaa cacaccacaa
     3541 attacgccga caaaaaagat aacagtgagg gctatcggtg aggaactgcc gcgtggtgat
     3601 taccaacgtt gcccgcaatg tgacatgctg tttagcctgc ccgagataaa ttctcatcaa
     3661 agtgcctatt gtccgcgctg tcaggcaaaa attcgtgacg ggcgcgactg gtcgctaacg
     3721 cgcctggcgg caatggcctt caccatgctg ttgctgatgc cgtttgcctg gggcgaaccg
     3781 ctgttgcata tctggctgtt aggcattcgt attgacgcca acgtcatgca aggcatctgg
     3841 caaatgacca aacagggcga tgcgataacg ggatcaatgg tctttttttg cgttattggt
     3901 gcccccctca ttctggtgac ctccatagct tatttatggt ttggtaaccg actgggaatg
     3961 aatctacgcc cggtactgct gatgcttgag cgacttaaag agtgggtaat gctggatatc
     4021 tacctggtcg gcattggcgt tgcttctata aaggtacaag attatgccca tatccaggcg
     4081 ggtgtcggct tgttctcttt tgtggcgttg gtgattttaa cgacggtgac gttgtcacat
     4141 ctcaatgtcg aagaactgtg ggagcgattt tatccacagc gccccactac gcgtagagac
     4201 gagaaactcc gtgtctgtct tgggtgccat tttaccggct atcctgatca gcgtggtcgc
     4261 tgcccgcgtt gccatatccc gctacgcctg cgtcgccgtc atagtctgca aaaatgctgg
     4321 gcggcgctgt tagcgtcaat cgttttgttg ttacctgcca acctgttgcc tatttctatc
     4381 atttatctga atggtggccg gcaggaagat acgattcttt ccgggattat gtcgctggca
     4441 agtagcaaca ttgccgttgc gggaatcgtg tttatcgcca gtattctggt accgtttact
     4501 aaagtgatcg tcatgttcac tttactgttg agtattcatt ttaaatgcca gcaaggttta
     4561 cgcacacgca ttctgttact gcggatggtg acctggattg gtcgctggtc gatgctcgac
     4621 ctgtttgtca tatctttaac catgtcgctg attaatcgcg atcagatcct cgcttttact
     4681 atgggaccgg ctgcgtttta tttcggcgca gcggtaattt tgactattct tgctgtggaa
     4741 tggctggaca gccgcttact ttgggatgca catgagtcag gaaacgcccg cttcgacgac
     4801 tgaagcgcag attaaaaata aacgccgtat ctcacctttc tggctgctgc cgttcatcgc
     4861 gctaatgatt gccggttggc tgatttggga tagttatcag gaccggggta ataccgtcac
     4921 catcgacttt atgtcggcgg atggtattgt cccgggccgc acccctgttc gttatcaggg
     4981 cgttgaagtc ggaactgtgc aggatatcag cctgagcgac gatcttcgta agattgaagt
     5041 caaggtcagc atcaagtctg atatgaaaga tgcgctgcgc gaagagacac agttctggct
     5101 ggtgacgcca aaggcctcac tggcaggtgt ctccgggctg gatgccctcg tcggtggtaa
     5161 ctatatcggc atgatgccag gtaaaggtaa agagcaggat cactttgtcg cactcgatac
     5221 ccaaccgaag tatcggctgg ataatggcga tctgatgatc cacctgcaag cccccgatct
     5281 cggttcactg aacagcggtt cattggtcta tttccgtaag atcccggtgg gtaaagtcta
     5341 cgactatgcc atcaatccca ataagcaagg cgtggtgatt gatgtcctga tcgagcggcg
     5401 ttttaccgac ctggtgaaaa aaggtagccg tttctggaac gtttccggcg ttgatgccaa
     5461 cgtcagtatc agtggcgcga aggtgaaact ggaaagtctg gcggcactgg ttaacggtgc
     5521 gattgccttc gattcacctg aagagtcgaa acctgccgag gcggaagata cctttggtct
     5581 gtatgaagat ctcgcccaca gccagcgtgg cgtaataata aaactggaac tgccgagtgg
     5641 ggccggatta accgccgact cgacgccgtt aatgtatcag gggctggaag tcggacagct
     5701 gactaaactg gatttaaatc ctggtggtaa agtcaccgga gaaatgaccg ttgatcccag
     5761 cgtcgttacg ctgttacggg aaaatacccg catcgaatta cgtaatccga aattatccct
     5821 tagcgatgcc aatctcagcg ccctgctgac cggaaaaacc tttgagttgg tgcccggcga
     5881 tggtgagcca cgcaaagagt tcgttgttgt gccaggcgaa aaagccctgc tacaagagcc
     5941 ggatgttctg acactgaccc tgacagcacc agaaagttac ggtattgatg ctggtcagcc
     6001 gctcattctc cacggtgtgc aagtaggcca ggttattgat cgtaaactca ccagcaaagg
     6061 cgttaccttt accgtcgcca tcgagcctca gcatcgcgaa ctggtaaaag gcgatagcaa
     6121 atttgtcgtc aacagccgtg tcgacgtgaa ggtggggctg gatggcgttg agtttctcgg
     6181 tgccagcgcc tcagaatgga ttaacggcgg gatacgtatt ctgccgggcg ataaaggcga
     6241 gatgaaagcc agctatccac tgtatgccaa tctggaaaaa gcgctggaga acagccttag
     6301 cgatttaccc accacaaccg taagtttgag tgcagagacg ctgccggatg tgcaggcagg
     6361 atcggtagtg ctgtaccgta aatttgaagt tggtgaagtt attactgtgc gtccgcgagc
     6421 taacgcgttt gatatcgatc tgcatattaa gccggagtat cgcaaccttc tgaccagcaa
     6481 cagcgtgttc tgggcagaag gtggggcgaa agttcagcta aatggtagtg gtctgaccgt
     6541 acaggcatcc ccgctctcca gagcattaaa gggggctatt agcttcgata acctcagcgg
     6601 tgccagcgcc agtcagcgta aaggcgacaa gcgtattctg tatgcttccg aaacagcggc
     6661 ccgtgcggtt ggcgggcaaa ttacgcttca cgctttcgat gccggaaaac tggcggtcgg
     6721 gatgccaatt cgctatctcg gtattgatat cgggcaaatc cagacgctgg atctgattac
     6781 cgcgcgcaat gaagtacagg caaaggcggt actttacccg gaatatgtcc agacctttgc
     6841 acgcggtggt acacgcttct cagtggtcac accgcaaatt tcggcagctg gcgttgagca
     6901 tcttgatact atcctccagc cgtatatcaa cgtcgaacca ggccggggca atcctcggcg
     6961 cgactttgaa ttacaagagg ctaccataac tgattcgcgt tacctggatg gcttaagcat
     7021 tattgttgaa gcgccggaag ccggttcgtt aggcatcggt acgcctgtgc tgttccgtgg
     7081 tctggaagtc ggtacggtta ccggaatgac gctggggaca ttgtcagatc gcgtgatgat
     7141 tgcgatgcgc atcagtaaac gctatcaaca cctggtgcgt aacaattccg tcttctggtt
     7201 ggcatcgggt tacagtctgg actttggtct gacgggcggc gtagtgaaaa ccggcacctt
     7261 taaccagttt atccgtggcg gcatcgcctt cgccacgcct ccgggtacgc cactggcacc
     7321 gaaagcccag gaaggcaagc acttcctgtt gcaggaaagt gaaccgaaag agtggcgtga
     7381 atggggaact gcgcttccca aataatgccc actgctccgg cgtgcctgcg ccggagcgtt
     7441 tatgctaaac tgcgcgcctg tttttttgcc agtggtatat gctcgtggcc caacacaccg
     7501 tttatttccc ggacgccttt ctgacacaaa tgcgcgaagc gatgccttcg acgctctctt
     7561 ttgatgattt tcttgccgcc tgtcagcgcc cgttgcgccg cagcattcgc gttaatacgc
     7621 tgaaaacctc cgttgctgat ttcctgcaat taactgctcc ttatggctgg acgcttacgc
     7681 caattccgtg gtgtgaagaa ggtttctgga ttgaacgcga cagtgaagat gcattgccgt
     7741 tgggtagtac cgccgagcat ttaagcggcc tgttttatat tcaggaagcc agttcaatgt
     7801 tgcctgttgc cgccttgttt gctgacggta atgcaccaca gcgggtgatg gatgtcgctg
     7861 ccgcgcccgg ctccaaaacg acgcaaattg ccgcgcggat gaataacgaa ggggcaatcc
     7921 ttgccaatga gttttccgcc agtcgggtaa aagtgttaca tgccaatatc agccgctgtg
     7981 gcatcagtaa tgttgcgctc acgcattttg atggccgcgt atttggtgcg gcagtgccag
     8041 aaatgttcga tgccattttg ctggacgctc cctgctccgg cgaaggcgtg gtgcgtaaag
     8101 atcccgatgc gctaaaaaac tggtcaccag aaagcaatca ggaaatcgca gcgacccaac
     8161 gggaactgat cgacagcgcc tttcatgcat tacgtcctgg cggtacgctg gtttactcga
     8221 cctgtacctt aaaccgggaa gaaaacgaag ccgtttgcct gtggctgaaa gagacttatc
     8281 ccgacgcagt agagttttta ccgcttggcg atctcttccc tggtgcaaac aaggcgctga
     8341 ccgaggaagg ctttttgcat gttttcccac aaatttacga ctgcgaaggc ttcttcgttg
     8401 ctcgtctgcg taaaactcag gcgattcccg ccttacccac cccaaaatac aaagtgggca
     8461 atttcccgtt cagcccggtg aaagatcgcg aagccggaca aattcgtcag gcggctgcaa
     8521 gtgttggctt aaactgggat gaaaacctgc gactctggca gcgtgacaaa gaactgtggt
     8581 tgttcccggt gggcattgaa gccctgatcg gtaaagtccg attttctcgc ttggggatta
     8641 aacttgccga aacgcacaac aaaggttatc gctggcagca tgaagccgtt attgcccttg
     8701 cctcccccga caatgtgaac gcttttgaac tgacaccgca ggaagcggaa gagtggtatc
     8761 gcgggcgcga tgtttacccg caagccgcgc cagtagcgga tgatgtattg gttactttcc
     8821 agcatcagcc gattggttta gccaaacgga ttggttcgcg actgaaaaac agctacccgc
     8881 gtgaactggt gcgcgatggg aaacttttta ccggtaacgc ctgacagcgc acaaaaaaag
     8941 cgcacttttt gactggcaca ttcggctgcc tcaactaggc tgaaaaatgg tgcgatcgga
     9001 ctggtcgtac cacaaccggc agctaaatgg agagcacgaa gatgaaaacc agtgtgcgca
     9061 taggcgcttt tgaaatcgac gacggcgaat tacacggcga atcgccgggt gatcgaacgt
     9121 taaccattcc ttgtaaatca gaccccgatt tatgtatgca actggatgcc tgggatgctg
     9181 aaaccagtat tcctgccctg ctaaatggcg aacactctgt cctttaccgt acccgttacg
     9241 atcaacaatc tgatgcctgg attatgcgtc ttgcctgatc caaaaagaac ccgtcggcat
     9301 ggcgggttat ttgtcctggt tatttccccg ttgtaaaatc tctcctaaac ttaacggtac
     9361 ggcaccacac ttcggggatg aaatgttcgc gctggtactt tttgtttgct acctggatgg
     9421 cggttgtgaa gatattgttg tggatgtcta caacacggaa cagcagtgtc tttattctat
     9481 gagcgatcaa cggatccgcc atggcggttg ttttccaatc gaggatttta ttgatggttt
     9541 ctggcgacca gcacaggagt acggtgattt ttaattattg caattgcaca agagtcagtt
     9601 cgcccccaaa gacagcaccg gtatcaatat aatgcaggtt gccaatatcc acgcgatgtc
     9661 gcaacggtgt atgaccaaac cagaaatgat cagcaccttt aattccctgc ccttttgggc
     9721 gttcacctaa tcgcgagcgg ctccacaaga cctgatgcaa atcaacgtcc ttttgccatt
     9781 cataaacatc atccggataa tcggcatggg caataacatg tttgccggtg cgactgtgta
     9841 cttcaagaat aaagggcaaa tgctgacatt tttccagcgc cgttttcgct tgtttctgtt
     9901 gattatctgc cagcgcaata aaccagtcgc cgccattcat gaaccacaaa gacatctgct
     9961 gggatgccag cgcatccatc gccatctgtt catgattgcc tcttaccgca cgaacccaat
    10021 gttgttccag taactgcagg caacgtaaac tttgcggccc acgatcgata acgtctccca
    10081 ctgagataag taaatctcgc cacggatcaa aacgacaatg ccataatttg cggcgcaact
    10141 gctcaagaca accgtgtata tcgcctgaaa gccagatatg tcgccattga tgacccgcaa
    10201 ttctctgata aacgggcgca ggctgtttca tcaatatttt cctcccgcgc taaagatcac
    10261 ataatcttaa caagaatgtt aaaaaacgct ggacgcagac agtagagtgt gtgttatggt
    10321 tgactataaa gtcagcgaag ggaatgcttc tggcttttaa cagataaaaa gagaccgaac
    10381 acgattcctg tattcggtcc agggaaatgg ctcttgggag agagccgtgc gctaaaagtt
    10441 ggcattaatg caggcttagt tgccttgctc tttaagaata gatgacgacg ccaggttttc
    10501 cagtttgcgt gcaaaatggt caataaaaag cgtggtggtc atcagcttaa atgttaaaaa
    10561 ccgcccgttc tggtgaaaga actgaggcgg ttttttattg gaaatcaaaa ggctatttta
    10621 ggtaattaac agagtttttc agctcgctct ataaacggtg ccagactcat tttttcgccg
    10681 ggattgttag gatcatcaat ctgaatcacc gaaatgggtt gggctttagt cttcccactg
    10741 gcaacttcct tttgtgcgat atcgtttaaa ggatactgca cgagggtact tgggttgatg
    10801 acatacaaag cattacccgg tcggcaagtc agcatcacct cttcgcgatt aaacgcccat
    10861 ttgtctttac ccacttcaaa acggctgacg gtaatcacct gcggtgccgc cagcgccgct
    10921 gcagaactgg tgagtaacag aaacgccaga atactttttt tcatcatttt cgtaatccat
    10981 caaaaaggtt cgagagtcgc aagcaggctg atgatcacca gcactaccgc acctatcgcc
    11041 cattcgagtt tagtcatcca cacaaaccag ggagccgcac gatcttcatc ctgcctcatg
    11101 cgcggtacaa gaacataacg attagccagc gcgatgacca ccataatcat caccaggatg
    11161 gctttgagca ttaagagttg gccccagtaa gtcgtaagcg tggggggaaa tccggtaatc
    11221 aacaatgcat taagcacgcc gcttgccagt acgccgatca ccgcaaaatg cccgcaccag
    11281 gaaaaacgca tcagcgcctg aatagcctgg tgtcgccaac ggcctttgat gagttgcata
    11341 caccagagca ccggcagtaa tcccccaaac cacgccgccg cacagatcag gtgaattgcg
    11401 tgattagtct gatggatttt cgccgttacc ccttcattca gcgtcgcatg cccgactccc
    11461 gccagcagaa taaattgcgc ggtagtgagc ataaatagca aacgtggcat attctgcggt
    11521 tgcataaggg caacgatcaa cgttacgagg gcgagaacaa tttgccataa ccagatgcca
    11581 ccaaactgcg tttgcaaaac agccagccaa atgtttggcg aaaatacatc tgaccatccc
    11641 gttcccatca acccgccctg aacggcgagc attgcagtgg cactgatcaa actccagacg
    11701 gcagcgtgct gttgtaaacg cagaaaacgc ttcgtcagca agcgacgaat cgttaagggg
    11761 gccagccagg caccgtacat tgcgaagcca aaaaccagca tcaacgaggt gaaatggata
    11821 aatcgcagcg cgatccaggt aaacgccagc atgatttatt tcacgctaaa ggtgtagtgt
    11881 cctttcgttt tatgcccgtc caccgaaaca acatgccagt caacggtgta ggttccgggt
    11941 ttcagcgaat cggccagtgg aacaatcagt tgcttttggt cctgctcatt ccgcttcgcc
    12001 ggtaatgttt taatattttc gttttttggc cccgtgattt ttgcaccact gaatcctgtt
    12061 tcaacgcctt ccgagaagtt taaagtgatt gcctgcgggg cagctgtcac ttgcgcattt
    12121 gccgcaggat actgatgcgt taaatgcgca tgtgcccaga cggaaggtgt caccaacgaa
    12181 gtggtcagga ttgccagcgc gtagcgaagg gagcgtgcag ttgaagccat attatctatt
    12241 cctttttgta ataacttttt tacagagcat aaccttgtct aatgtctgag tcgaggatca
    12301 tcaattccgg cttgccatcc aggctcactc ttagtaactt ttgcccgcga atgatgagga
    12361 gattaagaat gctgaagaat ctggctaaac tggatcaaac agaaatggat aaagtgaatg
    12421 tcgatttggc ggcggccggg gtggcattta aagaacgcta caatatgccg gtgatcgctg
    12481 aagcggttga acgtgaacag cctgaacatt tgcgcagctg gtttcgcgag cggcttatcg
    12541 cccaccgttt ggcttcggtc aatctgtcac gtttacctta cgagcccaaa cttaaataaa
    12601 acttatacag agttacactt tcttacataa cgcctgctaa attatgagta ttttctaaac
    12661 cgcacccata atttgcagtc attttgaaaa ggaagtcatt atgtcgttct ggaaagttgc
    12721 agcagcgcaa tatgagcctc gtaaaacctc actgaccgaa caggtagctc atcatctgga
    12781 gtttgtgagt gccgccgcca ggcaacaatg tcagctatta gtttttccat cgctttcttt
    12841 actggggtgt gattattcgc gacgtgccct tcctgcccca cccgatctct cgctgttaga
    12901 cccgctttgt tatgctgcaa cgacctggcg aatgaccatc attgccggcc ttcccgttga
    12961 atataacgat cgctttattc gtggaattgc ggtgttcgca ccgtggcgaa aaacaccagg
    13021 gatctaccat cagagtcatg gtgcatgtct gggccgtcgt tccagaacca tcacggtggt
    13081 cgatgaacaa ccggaaggta tggatatgga cccgacctgt tcactgttta cgacagggca
    13141 atgtctgggg gaacccgacc tactggcatc tacccgccgc ttacaatttt tttcacatca
    13201 gtactcaatt gccgtgctga tggcaaatgc ccgtggtaac agtgcactat gggatgaaca
    13261 tggtcgtctc atcgttcgcg ccgatcgtgg ttcattattg ttagtcggtc agcgttcatc
    13321 acagggttgg caaggcgata tcattccatt acgctaggct ttttcggcct ggagcatgcc
    13381 atgttgcgca ttatcgatac agaaacctgc ggtttgcagg gagggatcgt tgagattgcc
    13441 tctgttgatg tcattgacgg aaaaatcgtc aaccccatga gccacctggt gcgccccgat
    13501 cgtcctatta gtccacaagc gatggcgatt catcgcatca ccgaagccat ggtcgccgat
    13561 aaaccgtgga ttgaagatgt gatcccacac tattacggta gtgaatggta tgtcgcgcac
    13621 aacgccagct ttgaccgccg cgtactgcct gagatgcccg gtgagtggat ttgcactatg
    13681 aaactggccc gtcgtttgtg gccagggatc aagtacagca atatggcgtt atataaaaca
    13741 cggaagctca atgtacagac gccgccgggc ctgcatcatc accgcgcgtt gtatgactgt
    13801 tatatcaccg ctgcgttgct tatcgatatt atgaacacct ccggctggac ggcagaacag
    13861 atggccgata tcaccggacg tccgtcgttg atgacgacct tcacctttgg caaataccgt
    13921 ggcaaagcgg tttccgacgt tgccgaacgc gatccgggct atctgcgctg gttgtttaat
    13981 aacctggaca gcatgagccc ggagctgcgt ttaacactga aacattatct ggaaaatact
    14041 taatcctcag gcgtagcggg taatgttccc tgcgccagcg cgaccagaaa agcatattcc
    14101 atcgctacgc cttcgtacga tttaaagcga ccagatttac cgccatgacc tgagtccatg
    14161 tcggtacaga gcaataaaag atggtcatcg gttttcagct cgcgcaattt agcgacccat
    14221 tttgccggtt cccaatattg cacctgagaa tcgtgcaaac cggtcgttac cagtaaatgc
    14281 ggataagcct gtgcggtgac gttgtcatat gggctgtagc ttttcatgta ctcgtaatat
    14341 tgcggatcct gcggattccc ccactcttca aactcaccag tggtaagagg aattgattca
    14401 tcaagcattg ttgttacaac atcaacaaac ggtacctggg cgataacgcc gtggaataat
    14461 tccgggcgtt gattaattgc aacgcccatc aacatgcccc ccgcacttcc gcccatcgca
    14521 taacagagcg aaggagagcc atagcccagt tttaacaatg catcgcaggc atcaagataa
    14581 tcattaaacg tatttttctt cttcagaaat ttaccgtctt cgtaccattg ttgccccagc
    14641 tcaccaccgc cgcgaacatg gacaatggcg tagacaaagc cacgatctaa caaactcaag
    14701 cggctaaaac tgaaatcggc atcaatactt gcgccgtaag aaccgtagcc atacaccagc
    14761 agcgggttgt gtcctttgcg aaaatgtttg cgatgataga ccagcgaaac cggaacttcg
    14821 acgccatcac gggcgactat ccacaggtgt tcactgcggt aattcgccgc atcaaaacca
    14881 ggaacttccg tttgttttaa tacacgacgc tcaccggtat ccatatccag ttcaaacaaa
    14941 gtatctggtg tggtcatgga agaataacca taacgcaatc gcgcggtttc aggttctgga
    15001 ttgtaggcaa tccaggtcac ataggccgga tcatcaaagg caatgccaat gacttcccgg
    15061 gtcttgcggt tgatttggcg caaactggtt aacccgcgct gacgctcttc aaccaccagc
    15121 cagtcggtaa acagcgtaaa cccttccagc ataatgttat cacgtggcgg aattaactct
    15181 tcccactgtt gctcatcacg catacgggta cggtataagc caaagttttt accgtggcgg
    15241 ttggaacgca gataaaaccg atgctggtag tgatcaaggc tgtattcgtg atctttgcgg
    15301 cgaggcagaa aaacaaacgg ctcggcatcg gccatttccg cgtccagcag gcgaacttca
    15361 ctggtggtgg cgctggccag atgaatgacc acataatgct tcgaggtcgt tttatgcagg
    15421 ctgacgtaat aggtatcgtc tttttcttcg taaatcagtt tatcttgcga tgctggcgta
    15481 ccgatggcgt gacgccagac ctgataaggc agcagcgtca ctggatgctt gcgaacatag
    15541 tagaaagtcc aggagtcatt tgcccagaca aagctgggtt caacgttatc cagcagttcc
    15601 gggtaccagt tacctgtttc cagattacga aaacgaatgc catactgccg tcgggaaaga
    15661 aaatcttctg ccagtgccat aatggtgtta tctggcgtaa tcgccattcc gcccatcgaa
    15721 taaaactcac tatgagccgc gcgcttgttg gcatcgagca atgtttccca ctcgtcccac
    15781 tcttcactga acgccgattg acgctggtag atagcatatt cacagcctgg ttcataaata
    15841 tgccgatagc ggtagccatt tttgatgtag ggcgcagaaa cttctcgttg cggaatgcgg
    15901 tcgatgattt cctttaagat gcgatcctgc aaggcttgtt gcgaggccat cacccgatga
    15961 ccgtaactat tttcttgttg cagatagtcc aggactttcg gctgagaacg cgtatcgtcc
    16021 cgcagccagt agtaattatc gatgcgcgta tcgccatgaa gcgtcatggc gtggggaatg
    16081 cgggcggctt ttggtagcat gttattgttc tttctggttg aaacatctta taagggtggc
    16141 aaaactcacc ggggatgcaa gcgaaacagg gtagtcattg cttagatgat gacaggtaat
    16201 ggcgcggata tcgaatgtta tgcagacaga gaaaatcagc ctgctcaaat ggctgtgcgg
    16261 ttctggataa cccgaaataa tcagcttcag gctatccaga gagcggaatt actccgccaa
    16321 agtacgtttt tgctgttcga gatcgcgttc aatgccttca cgaacatcct gggggatttt
    16381 cagcgcatca cccagtgcat tcaggtaact gcgttccata aaatggtcaa tatcaatagc
    16441 cgcgcagctc aggaaataga tttccagcgc ctcttcttca ttgcggaccc cggtagccag
    16501 gcgttgtgga tccagcggtt gttcgattgc ctgctcaatg agtacacgcc ccttctcttc
    16561 cacaccggct tctcgcaatt gctggtcgat tgccgcacgt tctttggcat caatatggcc
    16621 atcactttta gcggcaaaga ccagcgcaag gatcaaacgt tccgtacgct catccagcgg
    16681 cgtactttgc gcgccaaact gcggttcatc ctgatgcgcc gcgcgaattt tatctttgta
    16741 tttattccac agcaccgtac ccgctaccgc tccgccgcca accagtaacg cgtttgtgcc
    16801 atatttcgta agaagtttac gtgctgattt atttgcgacc agcagcccag ccagtccgcc
    16861 taatgcgcca ggcactaaaa gtttgcccaa tccctgatcc gcagacgagg aggtagaaga
    16921 acggctttgc ccaagcaggg attgcagttg atttaaccag ttagccatat ttgctcctca
    16981 ataaccattt ttagtgcctg tagcatagcg gatgaggatg tcagaaaatg tatattgaga
    17041 caaaagatgc gcaaataagc ggggcggtag gccgttattc aaagaaattc gcgccaggta
    17101 aggtgttcac tgtatataag gtgataaggg tattttaggt gaaagtagtt tgacgttttg
    17161 agtggaactg tttgatgctg attgcccggt tcgacgctgc tgcgtcttct caggactacg
    17221 attgtgttgc gttgtagggc ggacaaggca ctcgcgccgt atccgccaac ggtcaatcga
    17281 attatcagaa gccattaacg ccgctgatat tccgccattc ccgctttgca gtccacgctg
    17341 acctggtaat gaatatcggc acttttacca cgtacggtta gcggtactga ccatttatca
    17401 tctttcccct gaatgtcctg caaactgacc catgccacgg gatcgggctg accgacaatt
    17461 ttttgatcat cagcccagcg cgccacgcga ttttgttggt aatcacgttt tacgctcgcg
    17521 gcaattccgg cagcatccag accttcacac tttgggaaag tgaccgactt gctggtttca
    17581 ttattggcag cgaaaactga tgcgcaggca gaaaccaaca acagccctaa aaacgcccct
    17641 ctttttttca tgtttttctc catagcacaa tgattcagga gaaagcatgg tacaaattgt
    17701 caggagcgca agttgcttca ggccgcgtgt gaggcatctt cctcttcgga ttcagcaccg
    17761 acgacctgtg gggacggtaa tttgccggtt ttcagaatgg tgctcagcac atctttttgt
    17821 tctgccagcc atagcgaaag ggcttcacgt tgctcgtctt ccatttgcac tggagaattt
    17881 gtcagccagg tgtcaagcag atccgccgta tcaagcattt tgtcataagc atcggcttcc
    17941 tttttgctgg taaacgacat tttctcttcg ccctcacgaa tgactacgta tttaacttca
    18001 accgccattt gcagcctctc ataataactg tgattttata cagtatattt cttctcgatt
    18061 gggaaatcaa catcaacaat aaagacacgc gcaaacgttt tcgtttatac tgcgcgcgga
    18121 ataaatcagg ggatattcgt tatgacgtta ttaggcactg cgctgcgtcc ggcagcaact
    18181 cgcgtgatgt tattaggctc cggtgaactg ggtaaagaag tggcaatcga gtgtcagcgt
    18241 ctcggcgtag aggtgattgc cgtcgatcgc tatgccgacg caccagccat gcatgtcgcg
    18301 catcgctccc atgtcattaa tatgcttgat ggtgatgcat tacgccgtgt ggttgaactg
    18361 gaaaaaccac attatatcgt gccggagatc gaagctattg ccaccgatat gctgatccaa
    18421 cttgaagagg aaggactgaa tgttgtcccc tgcgctcgcg caacgaaatt aacgatgaat
    18481 cgcgagggta tccgtcgcct ggcagcagaa gagctgcagc tgcccacttc cacttatcgt
    18541 tttgccgata acgaaaacct tttccgcgag gcggttgctg aaatcggcta tccctgcatt
    18601 gttaaaccgg tgatgagttc ctccggcaag gggcagacgt ttattcgttc tgcagagcaa
    18661 cttgctcatg catgggagta cgctcagcaa ggcggtcgcg ccggagcggg ccgcgtaatt
    18721 gttgaaggcg tcgttaagtt tgacttcgaa attaccctgc taaccgtcag cgcggtggat
    18781 ggcgtccatt tctgtgcacc ggtaggtcat cgccaggaag atggcgacta tcgtgaatcc
    18841 tggcaaccac agcaaatgag ccagctggct ttggaacgtg cgcaagagat tgcccgtaaa
    18901 gtggtgctgg cactgggcgg ttatgggctg tttggtgtcg agctgtttgt ctgtggtgat
    18961 gaggtgattt tcagtgaggt ctcccctcgc ccacatgata ccgggatggt gacgttaatt
    19021 tctcaggatc tctcggagtt tgccctgcat gtgcgagcct ttctcggact tccggttggc
    19081 gggatccgtc agtatggtcc tgcagcttct gccgttatcc tgccacaact gaccagtcag
    19141 aatgtcacgt ttgataatgt gcagaatgcc gtaggcgcag gcatacagat tcgtttattt
    19201 ggtaagccgg aaattgatgg cagccgtcgt ctgggtgttg cgctggcgac cgctgagagt
    19261 gttgatgaag cgattgagcg cgcaaagcac gccgccggac aggtaaaagt acagggttaa
    19321 acccggcaaa aaaacgctac aaaaatgccc gatcctcgat cgggcatttt gacttttaca
    19381 gcttagcgcc ttctacagct tcacgcgcca gcttagtaat gcggtcgtaa tcgcccgctt
    19441 ccagcgcatc tgccggaacc agccaggaac caccgatgca cagcacgctt ttcagcgcca
    19501 ggtagtcacg gtagttagcc ggagaaatac cacctgtcgg gcagaaacgg acctgggaga
    19561 acggacccgc gatcgcctgc agggctttca caccgccgtt agcttcagcc gggaagaatt
    19621 tgaactcttt caaaccgtag tccataccca gcatcagttc ggaaacagtg ctgatccccg
    19681 gaatcagagg aatagtccct tcggtagcag ctttcagcag cggctcggtc agacccgggc
    19741 taattgcaaa ctgtgcaccc gcttcagtga cttctgccag ctgctgtgga ttcagcaccg
    19801 taccggcacc cacaatcgct tcaggcactt ctttggcgat agcacggata gcgtcaactg
    19861 cgcactcggt acgcagagtc acttccagaa cgcgcacccc accagcaacc aacgcttttg
    19921 ccatcggcac cgcgtgttcc agttttttta ccacgataac cggtacaacc gggccggtgg
    19981 tcaggattga ttctgcactt gttttccagt ttttcatcag agttttctct cgcctgatta
    20041 caaatttgtc gtcttaaaaa gtgatacagg ttgcgccctg ttcggcaccg gacagttttt
    20101 cacgcaaggc gctgaataat tcacgtcctg ttcccacgcg tgacgcgctc aggtcaggaa
    20161 tgtgtggttc gcgagcagcc agttccgctt cgtctaccag cagcgtcagt tcgcctgtct
    20221 gtccattcac acgaatgatg tccccgtcgc gcacttttgc cagcagcccg ccatcgtagg
    20281 cttctggtgt tacgtggata gctgacggca ctttacctga agcgccggag agtcgtccat
    20341 cagtaactaa cgcaattttg aaacaccggt ccaataatac accaagtggc ggcatgagtt
    20401 tatgtaattc tggcattccg ttcgcttttg gcccctgatg acggacaaca acgacacaat
    20461 cgcggtccag caaacccgct tcaaaggccg gcataacgtc atgctggctt tcaaaaacaa
    20521 ccgcaggcgc ttcaatcacc tggttctcta ccggcacggc agaggttttc ataaccgcac
    20581 ggcccaggtt accgcttaac acttttgtcc caccatgatg agagaaaggt tgttcgaagg
    20641 aagcgatcac attgctgtcg agtgattttt ccgccccttc ccgccagtcc agttcaccat
    20701 tattcagcca tggttcaagg gtataacgag acagaccaaa gcctgccacc gtattgacat
    20761 cttcatgcag caggcctgct ttgagcagtt cacgcaccag aaccggtacg ccacctgccg
    20821 cctggaagtg gttaatatcg gccggaccgt tcgggtagag acgtgccatc agcggtacaa
    20881 catcagaaag gtcagagaag tcatcccagt taatctgaat accggctgcg cgcgccatcg
    20941 ccaccaggtg catggtgtgg ttagtggaac caccggtcgc cagcagtgca acgataccgt
    21001 tcaccaccac tttctcatcg atcatcttac cgatcggcat ccattcatta ccattaccgg
    21061 tcatgcgcgt aacctggcgc gcagctgcag cggtcaaagc atcgcgcagc ggagaatccg
    21121 gatgaacaaa agaagagcct ggcaactgca tccccataaa ctccaccacc atctggttgg
    21181 tgttggcagt accgtagaaa gtacatgttc ccggcgcatg ataagacgcg gcttctgact
    21241 ccagtagtgc catgcggtcc actttacctt cggcataaag ctggcgaata cgcacttttt
    21301 ctttatttgg caaaccgctt gccatcggtc cagacggcac aaacaccgca ggcaaatgcc
    21361 caaacgacag ggctgccatc gtcagacccg ggacaatctt gtcgcacaca ccgaggaaca
    21421 gagcaccatc aaacatgtta tgggacagcc ccaccgccgc agacatcgca atcacttcgc
    21481 ggcttaacag cgacaattcc attccatcct gcccctgggt gacaccatca cacatcgccg
    21541 gaacaccgcc tgcaacctga ccaaccgcat ttgcttcatg cagagcttta cgaatgattt
    21601 ctggatagtg ttcataaggc tggtgcgcgg agagcatgtc gttataggag gtgatgatgg
    21661 cgatattgtt acgcaacatg cttttcaaag aggctttgtc ttctggctgg caggcagcga
    21721 aaccgtgtgc caggttaccg catgccaact gcgaacgatg aacggtcgaa gttttcgctt
    21781 gttctatccg ggcgaggtaa gcagagcgag tctcgcgcga acgttcaatg attcgatttg
    21841 ttacgcgtaa caattgtgga ttcataaagg ctcctgaaat tgagttgtca gagcaggatg
    21901 attcacaaca cgtttcattc agaggattca tgactgaaac gcctgtaacc ggagctcata
    21961 gggcaaaaac gtttcagtca gtgtaataaa aaaagcctcg tgggtgaatc cgcacgaggc
    22021 ctgaaagtgt aaaaattgtt ctacaatctg tgcaagatca tgttaccggt aaaataacca
    22081 taaaggatga gcgcagatat tactcaaact cattccagga acgaccatca cgggtaatca
    22141 tcgccactga ggcaacgggt ccccaggttc cggcctgata cggtttcggc gcatcattgt
    22201 ccatcgccca cgcctcagta atggagtcta cccatttcca ggcttcttcc acttcatcgc
    22261 gacgtacgaa cagtgcctga ataccacgca tggtttccag cagcagacgt tcataggcat
    22321 ctgccagatg cgtttgatta aaggtttctg aatagctcag atccagcttg gtgatttgca
    22381 ggttatgttt atggtcaagg ccaggaactt tatttagtac ctggatatcc acgccttcat
    22441 caggttgcag acggatagtc agtttattct gcggcagatc ctgccacgac tctttaaaca
    22501 gattcagttc aggtgttttg aaatagacca cgacttcaga acatttggtc ggcagacgtt
    22561 taccagtacg caggtagaat ggcacaccgg cccagcgcca gttatcaatg tcgacgcgga
    22621 tcgccacgaa ggtttctgta ttgctgctct tgttcgcgcc ctcttcttcc agatatcccg
    22681 gcactttttt gccctgggcg aagcccgcag tatattgccc gcgtacggtt ttttcgcgca
    22741 cgttggagcg gtcaatgcgg cgcagagact tcagtacttt cactttttca tcgcggatgc
    22801 tgtctgcact caggtcagac ggcggagaca tcgcaatcat gcaaagaatt tgcagcaggt
    22861 ggttctggat catgtcgcgc atctgaccgg ctttatcaaa atagccccag cgcccttcga
    22921 tccccacttc ttctgccacg gtaatctcaa catgatcaat ggtgcgattg tcccagttat
    22981 tcacaaacag ggagttagca aaacgcagcg ccaacaggtt cagcaccgtt tctttaccaa
    23041 gatagtggtc gatacggtaa acctggcact cctcgaagta ttcgccaacc tgatcgttga
    23101 tttcctgcga ggtcgccagc gacgtcccca gcggtttctc catgactacg cgtgccggtt
    23161 tagcattcag ttttgcctca ccaagccctt tgcaaattgc gccaaaagtg ctgggcggca
    23221 tggcaaagta gttaatggtg atacgatttt tttgatccag catcgcgccg agacggctga
    23281 atgcagcagt gtcattgaca tcgagattac aaaaatccag acgtgcactc agggtgtccc
    23341 ataaaccttc atcaatggtt tctttcatga aagtttcgag cgcctcgcgg acaactttgg
    23401 tataagccgc tttatcccag tcagcacgcc ctacgccgat aatccgggtg tccgggttga
    23461 gctgaccggc tttttccagt tgatacaggg aaggcagcaa tttacgacgc gcaaggtcgc
    23521 ctttcgcgcc gaaaatgacc aggtcacagg cctgggctgt ttgcgttacc gccatgtcat
    23581 tctccttaag ttaactatcc cggtacttaa gccagggtat acttgtaatt ttcttacggt
    23641 gcactgtact gcttttacga gcttgcgaaa actgtaaacg cttatccacc cgtgcgatta
    23701 cggtaaaagc gcgcaaagtg cggcaaaact gataaaaaaa tcatcgtttt ccattgggta
    23761 aaaatctgac actgatcatg ttatgaaaaa aaataacaac ttttttatct gcttttgtca
    23821 ttaacacagc acacaggcgt aatatctgac aaaactgcat ttcgatttct ttcagagcgg
    23881 aaatcgtcat tacccgtgag tctctttaca tcatgaatat gctggaaaaa atccagtctc
    23941 agctggaaca tttgagcaaa tctgagcgca aagttgccga ggtcattctg gcttcgcccg
    24001 ataacgcgat ccattcgagt attgctgctc tggcgctgga agccaatgtt agcgaaccga
    24061 cggtgaatcg tttctgtcgc agcatggaca ctcgcggttt tcctgatttt aaacttcatc
    24121 tggcacaaag tctggcgaat ggcactccct atgttaatcg caacgtcaat gaagatgaca
    24181 gcgttgaatc atacacgggg aaaatatttg agtccgcaat ggcaacgctc gatcatgtcc
    24241 gtcattcact ggataaatct gccatcaacc gcgccgtcga tttgctcact caggcaaaaa
    24301 aaatcgcctt tttcggatta ggctcttcag ccgccgttgc ccacgatgcg atgaataagt
    24361 tctttcgttt taatgttccg gtggtgtact ccgatgatat cgtgctgcaa cgcatgagtt
    24421 gtatgaattg tagcgacgga gacgtggtgg tgctgatttc tcacacggga agaacaaaaa
    24481 atctggtcga gctggcgcag ctggcacgcg aaaacgacgc catagtgatt gccctcacct
    24541 ctgcgggtac cccgctcgcc cgggaagcaa cgctggcaat taccctcgac gtaccggaag
    24601 atactgacat ttatatgccc atggtttctc gacttgcgca gctgaccgtg atagatgtgc
    24661 tggcgacagg atttactttg cgacgcggtg caaaattcag agataacttg aagcgggtca
    24721 aggaagcgct gaaggaatcg cgttttgata agcagctact taatttaagt gacgatcgct
    24781 aaaaacgact gtcactgtcc taatcttata cgacatccga atgagattaa tttatcgcca
    24841 tcgcggcgtt atttcattcg gatttcatgt tcaagcaaca cctggttgtt tcagtcaacg
    24901 gagtattaca tgtccagaag gcttcgcaga acaaaaatcg ttaccacgtt aggcccagca
    24961 acagatcgcg ataataacct tgaaaaagtt atcgcagcgg gtgccaacgt tgtacgtatg
    25021 aacttttctc acggctcgcc tgaagatcac aaaatgcgcg cggataaagt tcgtgaaatt
    25081 gccgcaaaac tggggcgtca tgtggctatt ctgggtgacc tccaggggcc caaaattcgt
    25141 gtatccacct ttaaagaagg caaagttttc ctcaatattg gggataaatt cctgctcgac
    25201 gccaacctgg gtaaaggtga aggcgacaaa gaaaaagtcg gtatcgacta caaaggcctg
    25261 cctgctgacg tcgtgcctgg tgacatcctg ctgctggacg atggtcgcgt tcagttaaaa
    25321 gtactggaag ttcagggcat gaaagtgttc accgaagtga ccgtcggtgg tcccctctcc
    25381 aacaataaag gtatcaacaa acttggcggc ggtttgtcgg ctgaagcgct gaccgaaaaa
    25441 gacaaagcag acattaagac tgcagcgtta attggcgtag attacctggc tgtctccttc
    25501 ccacgctgcg gcgaagatct gaactatgcc cgtcggctgg cacgcgatgc aggatgtgat
    25561 gcgaaaattg ttgccaaggt tgaacgtgcg gaagccgttt gcagccagga tgcaatggat
    25621 gacatcatcc tcgcctctga cgtggtaatg gttgctcgcg gcgacctcgg tgtggaaatt
    25681 ggcgatccgg aactggtcgg cattcagaaa gcgttgatcc gtcgtgcgcg tcagcttaac
    25741 cgcgcagtaa tcacggcgac ccagatgatg gagtcaatga ttactaaccc gatgccgacg
    25801 cgtgcagaag tcatggacgt agcaaacgcc gttctggatg gtactgacgc tgtgatgctt
    25861 tctgcagaaa ctgctgctgg tcagtatccg tcagaaaccg ttgcagccat ggcacgcgtt
    25921 tgcctgggcg cggaaaaaat cccgagcatc aacgtttcta aacaccgtct ggacgttcag
    25981 ttcgacaatg tggaagaagc cattgccatg tcagcaatgt acgcggctaa ccacctgaaa
    26041 ggcgttacgg cgatcatcac catgaccgaa tcgggtcgta ccgcgctgat gacctcccgt
    26101 atcagctctg gtctgccaat tttcgccatg tcacgccatg aacgtacgct gaacctgact
    26161 gctctctatc gcggcgttac gccggtgcac tttgatagcg ctaatgacgg cgtagcggct
    26221 gccagcgaag cggttaatct gctgcgcgat aaaggttact tgatgtctgg tgacctggtg
    26281 attgtcaccc agggcgacgt gatgagtacc gtgggttcta ctaataccac gcgtatttta
    26341 acggtagagt aagtacgttg ccggatgcgg cgaaaacgcc tcatccggcc tacagttcaa
    26401 tgataattca acagatttcg aatcttctga agcaaacttg aacttatcac cagtcgaagg
    26461 cctctcctcg cgagaggcat tttttatttg atgggataaa gatctttgcg cttatacggt
    26521 tggatttcgc ccggtttgcg agttttcagc aattttaata tccaggtgta ttgttctggt
    26581 cgcggaccaa caaaaatctc gacttcttca ttcatccgcc gcgcaatcgt atgatcatcc
    26641 gcctctaaca gatcatccat cggtgggcgc acctgaatcg tcaaacgatg cgttttgcca
    26701 tcataaatcg gaaacagcgg tacaacgcgc gcacggcaca ctttcatcaa acgaccaatc
    26761 gcaggcaacg tcgctttata ggtggcaaag aaatcaacaa attcgctgtg ctctgggcca
    26821 tgatcctgat cgggtaaata atatccccag tagccctgac gtaccgactg gatgaatggt
    26881 ttaataccat catttctcgc atgcagacga ccgccaaagc gacgacgcac cgtgttccag
    26941 acataatcaa aaaccgggtt gccctgatta tggaacatcg ctgccatttt ctgcccttgc
    27001 gaggccatca gcatggcagg aatatcgacg gcccaaccgt gcggcaccag aaaaatcact
    27061 ttctcgttat tacgccgcat ctcttcgata atctccagcc cttgccagtc aacgcgcggc
    27121 tgaattttct ccggcccgcg tattgccaac tcagccatca ttgccatcgc ttgcggcgcg
    27181 gtggcaaaca tctcatctac aatcgcttcg cgttcagctt cactacgttc tggaaagcag
    27241 agcgacagat tgattaacgc acgacggcgt gagctttttc ccagtcgtcc ggcaaaacgt
    27301 cccagccgtg ccagaatggg atcacggaac tttggcggcg ttaaagcgat acccgccatc
    27361 gcggctacgc ccagccatgc tccccagtag cgcgggtggc gaaaggattt atcaaactca
    27421 ggaatgtatt cgctattatt ttttttcgtt tccatgcttt tccagtttcg gataaggcaa
    27481 aaatcaatct ggtgatagtg tagcggcgca acttgccccg caccaaataa aaaaagccgg
    27541 tactgactgc gtaccggctg cgaatggatg ttaattaatc aaaccgtagc tgcggcacaa
    27601 tctctttggc ctgcgccagg aattcgcgac gatcggagcc ggtcagccct tcggtacgcg
    27661 gcagttttgc cgtcagcggg tttacggcct gctggtttat ccatacttca tagtgcagat
    27721 gcggcccggt tgaacgtccg gtattaccgg aaagcgcgat acggtcgcca cgtttcacct
    27781 tctgtcccgg tttcaccagg atcttgcgca agtgcatata acgcgtggtg tagctgcgac
    27841 catgacgaat agccacataa taacctgctg cgccactgcg tttggcaacc accacttcac
    27901 cgtcacccac tgaaagcact ggcgtacctt gtggcatggc aaaatcaaca cctctgtgtg
    27961 gcgcaacgcg accggtcacc ggattagtac gacgcgggtt aaagttagat gagatacgga
    28021 actgtttcgc cgtcgggaat cgcaagaatc ctttcgccag accagtaccg ttacgatcgt
    28081 agaatttgcc atcttcagcg cggattgcgt aataatcttt accttctgaa cgcaaacgta
    28141 cgcccagcag ctggctttgc tcacgtttac catcaagcat ttctcgtgac attaacaccg
    28201 caaattcatc gccttttttc agtttgcgga aatccatttg ccactgcatg gctttaatca
    28261 ctgcgctcac ttcggcgctg gttaaaccgg cgtttctggc gctggcaaca aagcttcccc
    28321 cgacggtacc tttcagcaga ttgttgaccc actctccttg ctgcatttcg ctggtcattt
    28381 taaaaccgtt agcggcagta cggtcatagg ttcgggtttc acgacgagac acttcccagg
    28441 tgaggcgctg cagttcgccg tccgcggtta atgtccagga gagttgttga ccgattttca
    28501 ggttacgcaa ttctttgtcg gcagcagcca gttgggtgat atcacccata tcaataccat
    28561 actgattgag aatgctgctt agcgtatcgc cagtggaaac aacatattca tgcacgcccg
    28621 cttcaccggc gattttgtca tccagttcat cctggggaat ggcttcatct tcttgtgcag
    28681 cttgatcaat cggctcactg gcttcaggta agagcgaacg aatttcgttc tgttccagct
    28741 caatggtttt gacaattggc gtggcatcgc ggtgataaac atagggccgc cagacagcga
    28801 cggccagagt aagaacggtg agcgacccta acataacgcg gtgtggtcgc ggtaaattat
    28861 taaacgccag ggcgacagag cgggctatct gttgcacgta atcacttcct cattaatctc
    28921 ctttcaggca gctcgcatac tggttggcta attgattcag gaattctgaa tagcttgttt
    28981 tacccagttt gatattcgtc cccaggggat ccaacgttcc catacgaacg gatgtccctc
    29041 gtgcgacgct ctcaacgacc gctggcctga actgtggctc agcaaaaacg caggttgctt
    29101 tttgctcaac caactgtgtt cttatttcat gtaaacgctg cgcgccaggt tgaatctcag
    29161 ggttaacggt aaaatgacca agcggtgtca gtccgaactg tttttcgaag tagccgtaag
    29221 catcgtgaaa aacgaaataa cctttcccct tgagcggcgc gagctcgtta ccaacctgcg
    29281 tttcggttga ggctaattgt gcctcaaaat ccttcaggtt ggcgtcaagt ttggctcgac
    29341 tttgcggcat aagttccact aattttccat ggattgcaac cgctgtagcc cgcgctatct
    29401 ctggggaaag ccaaagatgc atgttgaaat cgccgtgatg gtgatcttcg tcactttttt
    29461 ccgcgtggtc gtgatcatca tcatcgtcgt gaatactttt catcagcagc ggtttcacat
    29521 cttctagctg cgcaatcgtt acctgtttcg cttctggtaa tttacttacc ggtttttgca
    29581 tgaacgcttc catctccggg ccaacccaaa cgactaagtc cgcgttctgt aagcgtttta
    29641 catccgatgg acgcagtgaa taatcatgtt ctgaagcgcc gtcaggcagt aaaacctgcg
    29701 tttctgttac cccatcagca atggcagaag cgatgaaccc aacgggttta agcgaagcga
    29761 caacggcggc atctgcggcc tgtgttgcac cgccccagag agcggcggat aatgctgcga
    29821 aaagaagcgt ttttttatgt aacataatgc gaccaatcat cgtaatgaat atgagaactg
    29881 tgatattata acatttcatg actactgcaa gactaaaatt aacatgacaa gtctggtttc
    29941 cctggaaaat gtctcggttt cttttggcca acgccgcgtc ctctctgatg tgtcgctgga
    30001 acttaaacct ggaaaaattt tgactttact tgggccaaac ggcgcaggta agtcgacact
    30061 ggtacgggta gtgctcgggc tggtaacacc cgatgaaggg gttatcaagc gcaacggaaa
    30121 actgcgcatc ggctatgtac cgcagaagct gtatctcgac accacgttgc cactgaccgt
    30181 aaaccgtttt ttacgcttac gccctggcac acataaagaa gatattttgc ctgcactgaa
    30241 acgtgtccag gccgggcatc tgattaacgc accgatgcaa aagctctcgg gtggcgaaac
    30301 gcagcgtgta ctgttagcgc gagcattgtt aaatcgaccg caattattag tgctggatga
    30361 acccactcag ggcgtggatg tgaatggtca ggtggcgtta tatgacctta ttgaccaact
    30421 gcgtcacgaa ctggattgtg gcgttttaat ggtatctcac gatctgcatc tggtaatggc
    30481 aaaaaccgat gaagtgcttt gcctgaatca ccacatttgt tgttccggca caccggaagt
    30541 tgtttccctg catccggagt ttatttctat gtttggtcct cgtggtgctg aacaactggg
    30601 tatctatcgc catcatcata atcatcgtca cgatttacag ggacgaattg ttttgcgtcg
    30661 gggaaatgat cgctcatgat tgaattatta tttcccggtt ggttagccgg gatcatgctc
    30721 gcctgtgccg cgggtccgct gggttcgttt gtagtctggc gtcgtatgtc ttatttcggt
    30781 gatacgctgg ctcatgcctc attacttggc gtcgcgtttg gtttgttgct ggacgtgaat
    30841 ccattctatg cggtgattgc agttacgctg ctgctggcgg gcggtctggt atggctggag
    30901 aagcgtccac agctggcgat cgacacgtta ttagggatta tggcgcacag tgccctgtcg
    30961 ctgggcctgg tggtcgttag tctgatgtct aatattcgtg ttgatttgat ggcttacctg
    31021 ttcggtgatt tactggcagt gacgccagaa gatctcatct ctattgcgat tggcgtggtc
    31081 atcgtggtgg ctattatgtt ctggcaatgg cgcaatttgc tgtcgatgac gattagcccg
    31141 gatctggcgt ttgttgatgg tgtgaaatta cagcgcgtga aattgttgtt gatgctggtg
    31201 acggcattga cgattggtgt agcgatgaaa tttgtcggcg cgttgattat tacttcactg
    31261 ctgattattc ctgctgctac tgcacgtcgc tttgcccgca cgccggaaca gatggctggt
    31321 gtcgctgttt tggtggggat ggtggcagtg actggcggtt taaccttttc cgcattttac
    31381 gatacacctg caggcccgtc ggtggtgcta tgtgcggcac tgttatttat tatcagtatg
    31441 atgaaaaagc aggccagcta atctatcgct gaacacattt gtcggatgcg gcgcgagcgc
    31501 cttatccgac ctatggttcg ctatctctgg tatatgcctg ataagacgcg aatagcggcg
    31561 catcaggcaa cactgccagt gtcggatgcg ccgcgagcga ccgatccgac ttacggcatt
    31621 tctggcggcg tgatgccgaa gtggttccac gcccgcactg tcgccatacg cccgcgcggt
    31681 gtacgctgca aaaagccttg ctgaatcaaa taaggttcca gcacatcctc aatggtttca
    31741 cgttcttcgc caatggcagc cgccaggtta tccagaccta ccggcccacc aaagaactta
    31801 tcgattaccg ccagcaacaa tttgcggtcc atataatcga aaccttcggc atcgacattc
    31861 agcatatcca gcgcctgagc agcgatatca gccgagatgg tgccatcgtg cttcacttcg
    31921 gcgaaatcac gcactcgacg cagcagacgg ttagcaatgc gcggcgtacc gcgagcgcga
    31981 cgagcaactt ccagcgcgcc gtcatcactc atctcaagcc ccataaagcg tgcgctgcga
    32041 ctgacgatat attgcagatc cggcacctga taaaactcca gacgctgcac aataccaaaa
    32101 cgatcgcgca acggtgatgt cagcgaacct gcgcgcgtgg ttgcgccaat cagtgtaaac
    32161 ggcggcaaat caattttaat ggagcgtgcc gccggacctt caccaatcat gatatccagt
    32221 tggtagtctt ccattgccgg atacaacacc tcttccacca ctggtgaaag acggtggatc
    32281 tcatcaataa acagtacatc gtgtggttca aggttagtga gcattgctgc cagatcgccc
    32341 gccttttcca gcaccggacc agaagtcgtg cgtaaattaa cgcccatttc attggcgaca
    32401 atattggcaa gcgtagtttt acccaacccc ggaggaccaa aaatcaacag atgatcgagg
    32461 gcatcgccgc gcagtttcgc tgctttgatg aaaatctcca tctgtgaacg aacctgcggt
    32521 tgaccaacat actcttccag taatttaggg cgaatggcgc gatctgccac atcttccggc
    32581 aaagtggtac cggcagaaat cagacggtct gcttcaatca tcctttacct cataacgcgg
    32641 cgcgtagggc ttcgcgaatt aatgtttcac tgctggcgtc agggcgagcg attttgctca
    32701 ccatacggct tgcttcttgt ggtttatagc ccagcgccac cagcgcagca accgcttcct
    32761 gttcagcatc gtcggtcgcc gggctggcag gtgacgtgag taccaggtcg gcggctggcg
    32821 taaagagatc gccatgcaaa cctttaaatc ggtctttcat ttcgacaatc aagcgttcgg
    32881 cggttttttt gccaataccc ggcagtttca ccagtgcccc cacttcttca cgctcaacgg
    32941 cattaacgaa ctgctgcgct gacattccgg agaggatcgc cagcgccaac ttcgggccga
    33001 cgccgttggt tttgatcaac tctttgaaca acgtgcgctc ttgtttattg ttaaaaccgt
    33061 acagcagttg cgcgtcttca cgcaccacaa agtgggtgaa aacgatcgct tcctgacccg
    33121 cttcagggag ttcataaaaa caggtcatcg gcatatgcac ttcatagcct acgccgccca
    33181 cttcaattaa caccagcggg ggttgttttt caatgatgat gcctctgagt ctgcctatca
    33241 catgacgctc ctgcgtaatg aatcaaagat aatgctgtat gataaaaaaa tgctggatag
    33301 atatccagcg aaggatgaag aaaacttgcg aggtgtcccg atgatctgaa aaatggcgca
    33361 gtataattta ttctacagat tatattggaa gcaaatattt aaatattaca tattcagcga
    33421 agaaatgtgt aataaaaata cacatcgcga cccctgaaaa aataaatttt ttatgctatt
    33481 acgtatattc atatctattt caatggaatg acaacgtgaa tgttaattat cctgctgaat
    33541 atgaaattgg tgatatcgtc tttacatgta taggtgctac cttatttggt caaatatcag
    33601 ctgcatcaaa ttgctggagt aatcacgtcg ggatcattat cggtcataac ggtgaagact
    33661 tcctggttgc agaaagccgg gttccccttt caaccatcac cacgctatct cgttttatta
    33721 aacgttcgtc taatcaacgc tatgctataa agcgattaga cgctgggctg acagaacaac
    33781 aaaaacaacg aattgttgaa caggttccct cccggctacg caaactttac cacaccggtt
    33841 ttaaatacga atcttcgcgc cagttctgtt caaaatttgt ttttgatatt tataaagaag
    33901 cgctatgtat tccggtgggt gaaatagaga cgtttggaga attgttaaat agcaatccga
    33961 atgcaaaact cactttctgg aaattctggt tcttaggttc tattccgtgg gagcgtaaaa
    34021 ccgtcacgcc agccagtttg tggcatcatc cgggtttggt gttgattcat gcggagggag
    34081 tggaaacgcc tcagccggaa ctgaccgagg cggtataact taacgcagtc gccctctcgc
    34141 caggttcagc cgcgattcgc tcatctgcat cgcattctga ctgacgtgac aatgggtaat
    34201 cgcgatagct aatgcatcgg cagcatccgc ctgtggatta gcgggcagtt tcagcaaggt
    34261 gcggaccata tgctgcacct ggcttttttc ggcactacca atacctacca ccgtttgctt
    34321 tacctgacgt gccgcatatt caaataccgg caattcctga ttcaccgctg ccacaatcgc
    34381 cacgccgcgc gcctgaccca gtttcagggc tgagtcagcg ttcttcgcca taaagacttg
    34441 ttcaatggcg aaataatcag gctggaactg ggtgatgatt tccgtcacgc ccgcatagat
    34501 gagcttcaga cgagacggta aatcatccac tttggtgcgt atgcatccgc tacccaggta
    34561 ggacagttgc ctgcctacct ggcggatgac gccatagccg gtcacgcgcg aacccgggtc
    34621 aatgccgaga ataatagcca tcacgcgtct ccgttttgct gtttagcagg cctcatcaga
    34681 gagtcgctgc aacctcatca gagatttcac cgttatggta aacttcctgc acgtcgtcgc
    34741 aatcttccag catatcgatc agacgcatca gtttcggtgc ggtttctgca tccatatcag
    34801 ctttggtgga cgggatcatg gaaacttccg cgctgtctgc tttcagacct gccgcttcca
    34861 gagcgtcgcg tactttgccc atttcttccc atgcagtgta gacatcaatc gcgccgtcat
    34921 cataggtcac aacgtcttca gcaccggctt ccagggctgc ttccatgatg gtgtcttcat
    34981 cgcctttctc gaaggagatc acgccttttt tgctgaacag ataagctacg gaaccatcag
    35041 taccgaggtt accgccacat ttactgaatg catggcgcac ttcagcaacg gtacggttgc
    35101 ggttgtcaga cagacattca atcatgattg ccgtgccgcc aggaccgtaa ccttcgtaga
    35161 tgatggtttc catgtttgca tcatcatcac cgcccacacc tcgtgcaatt gcgcggttca
    35221 gagtgtcacg ggtcatgttg ttagacagtg ctttatcaat tgctgcacgc agacgcgggt
    35281 tagcgtccgg atcaccaccg cccagcttag ccgcggttac cagctcacga atgattttag
    35341 tgaagatttt accgcgctta gcatcctgcg cagctttacg atgtctggtg ttggcccatt
    35401 tactatgacc tgccataaaa atatctccag atagccctgc ctgttcaggc agcgttaatt
    35461 acaaactgtt caatcgcctg ccggttgctc caggacttag tgagcgccgc agcagcaggc
    35521 gcgtcaagcc acttgtaagc cagatgttca gtgaaaacga tctggcgctc gtgcgggagc
    35581 gcaagacaga accatgattc cgtattacgc gtcacgcccg gcgcatagcg atgacgtaaa
    35641 tgtgaaaaaa tttcaaactc taccgtgcgc tgacagtcaa ttaaggtcag ttgttcagcg
    35701 acaacatcaa tggtgacctc ttcctttact tcgcgcatgg cagcttgcgg cgcggtttca
    35761 ccctcttcca cgctgccggt taccgactgc cagaaatcgg gatcgtcacg ccgctgcaac
    35821 atcagcaccc gtttcgtatc ttgtgcatag atgaccacta agatcgaaac gggacgctta
    35881 tacactttat ccttcacgct gcctcttcgt tgactgcctt cgctcatccc attcacatag
    35941 ttatctatgc tcatgggagt tcactcagtt gccgcctcga tgcaacgcga atgatttcgt
    36001 gtatttgagt catatcagtt attctcgtcc ttcttcacaa cctgaatgct cagctcagcc
    36061 agtgcagtcg ggttagcaaa gctcggtgct tcagtcatca gacacgctgc cgccgtggtt
    36121 ttcgggaagg cgataacgtc acggatattg tcggtgccgg tcagcagcat ggtcagacgg
    36181 tcaagaccga atgccagacc tgcgtgcggc ggagtaccgt atttcagggc gtcgagcagg
    36241 aagccgaatt tctcgcgctg ttcctcttcg ttgataccca gaataccaaa caccgtctgc
    36301 tgcatatcac cattatggat acgcacagaa ccaccgccca cttcgtaacc attgatgacc
    36361 atatcgtaag cgttcgccac cgcattttct ggtgcagctt tcagttctgc agcagtcatg
    36421 tctttcggtg aggtgaacgg atggtgcatt gccgtcaggc cgccttcacc gtcgtcttca
    36481 aacatcggga agtcgataac ccacagcggt gcccatttgc tttcgtcggt cagaccaagg
    36541 tctttaccca ctttcaggcg cagtgcgccc atcgcgtcgg caacaatttt cttgttgtcg
    36601 gcaccgaaga aaatcatatc gccatcttgc gcggcagtac gatccaggat ggcttcgatg
    36661 atttctgcat taaggaactt cgctaccggg ctgttgatac cttccagacc tttcgcgcgt
    36721 tcgttaactt tgatgtaagc cagacctttc gcgccgtaga ttttaacgaa gttaccgtat
    36781 tcgtcgatct gcttacgggt caacgatgcg ccgcccggaa cacgcagagc ggcaacacgg
    36841 cctttcggat cgttcgccgg acctgcaaat actgcaaact caacagattt cagcagatcg
    36901 gcaacgtcgg tcagttccat cgggttacgc agatccggtt tatcagaacc ataacggcgt
    36961 tctgcttctg caaaggtcat taccgggaaa tcgcccagat ccacgccctt cacttccagc
    37021 cacagatgac gcaccagcgc ttccatcact tcacgcactt gcggcgcggt catgaaagaa
    37081 gtttccacat cgatctgagt aaattcaggc tgacggtcag cacgcaggtc ttcgtcacgg
    37141 aagcatttaa cgatctgata gtagcggtca aagccggaca tcatcagtag ctgtttgaac
    37201 aactgcgggg attgcggcag cgcgtagaat ttacctttgt gcacacgaga aggcaccagg
    37261 tagtcacgcg cgccttccgg cgtggctttg gtcagcatcg gagtttcgat gtcgaggaag
    37321 ccgtggtcat ccataaaacg gcgcaccagg ctggtgattt tagcgcgggt tttcaggcgc
    37381 tgagccattt ccgggcgacg caggtcgagg tagcggtatt tcagacgcgc ttcttcggtg
    37441 ttgacgtggt tagagtcaag cggcagaaca tctgcacgat tgatgatagt cagcgaggac
    37501 gccagtactt cgatttcgcc agtcgccata tcgcggttaa tatttttttc gtcacgcgca
    37561 cgtacggtgc ccgtgacctg aatgcagaac tcattacgca gttcagaggc cagctttaac
    37621 gcgtccgcac gatccggatc gaaaaatacc tgcacgatac cttcgcggtc gcgcatatcg
    37681 atgaagatca ggctaccaag atcacgacga cggttgaccc aaccacacag agtcacctgc
    37741 tgccccacgt gggacaaacg gagctgtcca caatattctg tacgcatgag atatccctta
    37801 acttagctgc cggcggatgc cccctgctgc gcaggtgacc aagtcgcagc gttagctgta
    37861 tgtcacaact gaatgaaaaa aggcggctat tatactggaa attctgccgc accgtaagag
    37921 cctggcccgc gctggaacgc ctcgttacca ctttatatcg ggcctgaaat cagactctac
    37981 gccagtttgc tataaaggtg ttgcccgaac tcataaaaat taacaaaatt tgtcgttccg
    38041 ccatcggcta atcgcattaa ggtgagaggc acgattttgt tttgtcagga gtcatcatgc
    38101 ttgaacttaa tgctaaaacc accgcgctgg tggtgattga tttacaagaa ggcatcttac
    38161 cttttgccgg aggtccacat actgccgatg aggtggttaa tcgcgccggg aagctggcgg
    38221 cgaaatttcg cgccagcggt cagcccgtgt ttctggtgcg cgttggctgg tctgccgatt
    38281 acgccgaagc attaaaacag ccggttgatg ccccctcccc cgcaaaagtg ttgcccgaaa
    38341 actggtggca acatcctgct gcattaggtg caaccgacag cgatatcgaa atcatcaaac
    38401 gtcaatgggg tgcgttttac ggtacggatc tggagttgca attacgccgc cggggtatcg
    38461 atacaatagt gttatgtggg atctcgacca atatcggtgt tgaatccacc gcccgcaatg
    38521 cctgggaact cggttttaat ctggtgattg ccgaagatgc ctgtagcgcc gccagcgccg
    38581 agcagcacaa taacagcatt aatcatatct acccgcgcat cgcccgtgtg cgcagtgtgg
    38641 aagagatcct ccacgcgtta tgatttacat cggtctgccg caatggtcgc atcctaaatg
    38701 ggtgcggttg gggatcacca accttgaaga gtatgcccgc cactttaact gcgtggaggg
    38761 taacaccacg ctttatgccc tgccgaaacc cgaggttgtc ctgcgctggc gtgagcagac
    38821 cacagatgac ttccgcttct gttttaagtt tccggcgacc atttcgcatc aggcagcatt
    38881 acggcattgc gatgatttag tgactgaatt tttgacccgc atgtcaccgt tggctccgcg
    38941 cattggacaa tactggctgc aactgcctgc cacattcggc ccacgagagc tgcctgcgct
    39001 ttggcatttt ctcgattctc ttcccggtga atttaattat ggggtggaag tccgccatcc
    39061 acagtttttc gccaaagggg aagaggaaca aacgcttaat cgcggtttac atcagcgcgg
    39121 cgttaatcgg gtgattttag acagccgccc ggttcatgca gcacgtccac acagtgaagc
    39181 tattcgcgac gctcaacgaa aaaaacctaa agttccggta catgctgtac tgacggcgaa
    39241 aaatccactg atccgtttta tcggtagtga tgatatgacg caaaaccggg agttatttca
    39301 ggtctggtta caaaaattag cacagtggca tcagaccact acgccttatc tttttttaca
    39361 tacgccagat attgcccagg ccccggaact ggtacatacc ctgtgggaag acttacgtaa
    39421 aacgcttcca gagatcggag cagttccggc tattccacag caatcttctc ttttctgaat
    39481 ttgccaccta tcatagacag gtgccatcgg ccattttaaa gggagtttgt atggtaagcg
    39541 cgctgtatgc cgttttaagt gcgttgttat taatgaagtt ctcttttgat gtcgttcgcc
    39601 tgcgaatgca gtaccgcgtt gcctatggcg acggcggttt tagcgaactg caaagcgcta
    39661 ttcgcattca tggtaacgcg gtggaatata ttcctatcgc gattgtgttg atgctgttta
    39721 tggaaatgaa tggcgcagaa acctggatgg tgcatatttg cggcatcgtt ttgcttgctg
    39781 gtcgtctgat gcattattac ggttttcatc accgtctgtt ccgctggcga cgttctggca
    39841 tgagcgcgac ctggtgtgcg ctgttgctga tggtgctggc gaatctttgg tatatgccct
    39901 gggagttggt tttctccctg cgttagcgca caatacgcca ctttcttttt cccggatttt
    39961 tacgttatgt ctcaccgcga cacgctattt tctgccccta tcgccagact gggcgactgg
    40021 acctttgatg aacgggtagc tgaagtcttc ccggatatga tccagcgttc cgttcccggc
    40081 tattccaata ttatttccat gattggtatg ttagccgagc gcttcgttca acctggtacg
    40141 caggtttacg atctgggttg ttctctgggc gcggcgacgc tctcggtgcg tcgcaacatt
    40201 catcatgata attgcaaaat tattgccatc gataactccc ccgcgatgat tgaacgctgc
    40261 cgtcgtcata ttgacgccta taaagcccct acgccagtag acgtcattga aggtgatatt
    40321 cgcgatatcg ccattgaaaa cgcatcgatg gtggtgctga attttaccct gcaattcctg
    40381 gaaccttccg agcgccaggc gttactggat aaaatttatc aagggctgaa cccgggcggc
    40441 gcgctggtgc tttcggaaaa attcagtttc gaagatgcca aagttggtga actgttgttc
    40501 aacatgcacc acgactttaa acgtgccaac ggttacagcg aactggagat cagccagaag
    40561 cgcagcatgc tggaaaacgt gatgctgacc gattccgtgg aaacccataa agcacgcctg
    40621 cataaagctg gttttgaaca tagtgagctg tggttccagt gctttaactt tggttcactg
    40681 gtggcattaa aagcagagga cgctgcatga tcgactttgg taacttttat tctctgattg
    40741 ccaaaaatca tctttcacac tggctggaaa cgctgcccgc gcagattgct aactggcagc
    40801 gcgagcagca gcacgggctg tttaagcagt ggtccaacgc ggtggaattt ctgcctgaaa
    40861 ttaaaccgta tcgtctggat ttattgcata gcgtaaccgc cgaaagcgaa gagccactga
    40921 gcgccgggca aattaaacgc attgaaacgc tgatgcgcaa cctgatgccg tggcgcaaag
    40981 ggccgttctc actgtatggc gtcaacatcg ataccgaatg gcgttccgac tggaaatggg
    41041 atcgcgttct gccccatctt tctgatttaa ccgggcgcac cattcttgat gtcggctgtg
    41101 gcagcggtta tcacatgtgg cgcatgattg gcgcaggcgc gcacctcgcg gtgggaatcg
    41161 accctacgca actgtttctg tgccagtttg aagcggtgcg taaactactg ggtaacgatc
    41221 agcgcgcaca tttgttaccg ttaggtattg aacagcttcc ggcattgaaa gcctttgata
    41281 ccgtcttttc gatgggcgtg ctttatcatc gtcgttcacc gctggagcat ctctggcagt
    41341 taaaagacca actggtgaat gaaggtgaac tggtgctgga aacgctggtt attgatggtg
    41401 atgagaacac ggtgctggtg ccgggcgatc gttacgcgca aatgcgtaat gtctatttca
    41461 tcccttccgc gctggcgctg aaaaactggc tgaagaagtg tggttttgta gatatccgca
    41521 ttgcggatgt gagcgttacc accacagaag agcagcgacg caccgaatgg atggtcaccg
    41581 agtctctggc cgattttctc gacccgcatg atccgggtaa aacggtggaa ggttatcctg
    41641 cgcctaaacg cgcggtgctg attgcgcgca agccgtaaag gtctggtatt actgccggat
    41701 gcggcgtgaa cgccttatcc ggcctacaaa accttgctaa ttcaatatat tgcagggact
    41761 atgtaggcct tataagcata gcgcatcagg cagttttacg tttgcataac ctcagcgccc
    41821 gtttccgggc gcttttcacg tcttatgcct gaacagctgg ctgatcaaac gccgttaact
    41881 tcggtgcatt gcccgtatat ttttcgatat acaccagcgc agagttaccg gcacaggcat
    41941 ttgccagctg agagctgggg atatccgccg ttaacacgtt cgcactgccg tttttacaca
    42001 agccattttc cagatctggc catgcacctt catgcacgca taccacgcct tttttgatcc
    42061 cgtcagtcac caccgcgcct gtcagaatct gaccacgttt gttccagaca cgcaccagat
    42121 cgccattcgc aataccaaag cgagcagcat cttcggtgtg aatagtgatc ggttcacgat
    42181 cggcaaccgc atatttttta cgcagttccg catagttaag ctgactgtgt aaacggtgtg
    42241 ccggatgtgc ggtcagaagc tgcaactgct tctcgtcggc ggaacctttc cactcatcag
    42301 gcgcaagcca ggttgggtgt gccgggcaat ccttatagcc aaatttttcc agcgttttgg
    42361 agtaaatctc aattttgccg cttggcgtac ccagcgcatt tttcaccgga tcggcgcgga
    42421 aatcaccata acgaacgtat tgttcgttct tctcactgcg gcgcatttcg atcagtttat
    42481 tttgctgcca gaaagcatta aacatcggca tagtcacgcg ttgcgcacgg gcacctttct
    42541 gagcggcatc atagaaaaat ttcagccacg ccatttcatc tttaccttcg gtatagatct
    42601 cttttccgcc aggtttgagt aattcagcaa gatcggcaaa cacgtcaaaa tcgttgcgcg
    42661 cttcaaattg cggagcgaca gcctgcttca tcggcacaat atgctggttg ctgtaatcac
    42721 cggtcatcgt caggtcattg cgctcaaacg atgtggtgat cggtaatacg atatctgcat
    42781 gtttagcagc tgcggtccag tagcattcag aaacgacgat catctccggt ttctgccatg
    42841 ctttaatcag acgattagta tcctggtgat gggtaaagtt accgccaccc gcccaccaga
    42901 tcattttgat attcggatac gtttgttctt taccgttatg ctgatatttg ccgccaggat
    42961 tttccagcgc atcgacgata cgggcaacag gaatcgccgt cattccgcca tcatcagcgg
    43021 cctcgctggc ctgtccggca atcgccgcag acatttccgg cagcacgccg ccaacacgcg
    43081 tcgggttgcc accgttggag taatgataag agaatccaaa accgccgccc ggtgtaccaa
    43141 tttgccctaa catcgccgcc agcgtcacca gcatccagtg tttctgttca ccgtattgct
    43201 ggcgttggat cccccagcct gccatcagca tcgtgcgatt cgccgccatc agttctgcaa
    43261 gtttgacaat ctgcgcttcc ggtacgccag taatttccgc cgcccaggcc gcgctcttcg
    43321 gcgtattatc gctcttacct gtcagatact cttcaaactg cggatacccg gtagtgtatt
    43381 tttcaaggaa cactttatcg tgtttgcctt gtgtcatcag ggtatgcgca atccctaaca
    43441 tcagtgccac gtcggtgccc atattcggtg cgatccaggt ggcattatcg ccaaagaatt
    43501 cgatggtttc ggagcagata ggatcaatgg caatcactgg tttgccagat tttttcagct
    43561 gatggaagta ttccagccct tgctcatcgg tactgctcca ggcaattttg agcgtgttca
    43621 gcgggttcat cccccacagc accacaacct ggctgttttc cagaatcagc ggccaggagg
    43681 tctgctgttc atacacctct acagaaccga ccacatgcgg catgatcacc tgtgccgctc
    43741 cggttgaata atcgccgcta tgcccggaat aaccgcccgc caggttcata taacgttgca
    43801 gtaaggtttg cgctttatgc aacacgccag aagaacgcca gccgtaagat ccggcaaaaa
    43861 tggccgatgg tccgttagct ttacgaatac gatcgtgttg ctcatgaatc agttttaatg
    43921 cttgttccca gctcacctgt acataggtat cttcgccacg acctttcgcc ggttgcagtg
    43981 gattatcgag atagcttttt ctcaccatcg gatgctgaat acgcgccgtg gtgtgtacct
    44041 gatccgccgc cgtagactgt aaggagttcg gtatggtttt cgccagcgcg cctgtcgaag
    44101 aaacaatctt gccgtccttc acttctacgt tcatcgctcc ccaacggccc gcggtgagaa
    44161 ttttgccgcc cttctcttct gcccatgcag gtaacggtgc agccgatgtc accaccagcg
    44221 cccctgccgc aataccgcta tgtttaataa attcacgtct tgttaatgtc ataacttcct
    44281 ccctgatcaa cgaggatcac tgtttctcgg taatatcttt ggcgttgtac tggaagtacc
    44341 gcgtcaaaat gtccagttcg ttttcgctca tgctggttcg cgcacccatt cctttggcaa
    44401 tggacggcca tgcattgacg gtgtaatggt cggaggcaat aggggcatgg caaccagcgc
    44461 aataggtatc gtcaagtttt tcagcgtatt gccatagagg tttacggtcc gctaatgcgg
    44521 gatcggtaag cacaccctgt aaagacgcct gacgccattg attgccgtat tcgtcagcct
    44581 gccattcccc gtttaccgtc agcgccttga taccttcttc acttaatgtg gctagcgcca
    44641 gccgttgacc tgccgccagg tagagcgtgt tttcactgcc ctgcatttga taaccctgca
    44701 acagaacgat cggctgtttg ccactggcat caacgacggt gagatcggta ccaggattca
    44761 cggtagccag ttcgcctatg cgagaagttt tgaaaggata aatatgtgcg ccattagtaa
    44821 ctgaagtagc ggcctgactt tccagctcat gcgccgcgtt gtcatccatt tttatttctg
    44881 gcggaaaatg ggcaatgcct ttatggcaat cgatacaggt ttcgccgtcc ttttgcgctt
    44941 tattatgcat tttctgcgca gattcacttt gcgaggcaat atccatggca tcaaaagaat
    45001 ggcaactacg gcaggttgca gagtcagtgg cttttaattc tttccatact gtttcggcca
    45061 tttcctggcg atgagcttcg aacttatcat cactgtctat tttgccgcta acaaattcat
    45121 gataaatatc tttagatgct tttaatttag caaataaata atccatccct gactttggaa
    45181 tatggcaatc ggcacattcc gcacgtatcc ctttctggtt tgaaaagtgg acagttccct
    45241 gatattcctc aaaaggttta ctcatcgagt ggcaggaaag gcaaaatgcg gtatccgacg
    45301 ttttatgtaa ggctttttgc gccagtaata acccgccgcc accaaccaca accgctatca
    45361 gcaaaaacaa taacccaatg cgttttttcc ctcgcataga ttcctctaat taaattccag
    45421 gacaccgata ccattgtata aataaataca caacgaattg actacttgct taattcggca
    45481 caacactgcc tgaaataatc g
//
LOCUS       DAZKGF010000018        44389 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZKGF010000018 DAZKGF010000000
VERSION     DAZKGF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.018"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            220..513
                     /gene="yicS"
                     /locus_tag="ACHOQV_000973"
     CDS             220..513
                     /gene="yicS"
                     /locus_tag="ACHOQV_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFP9629387.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(554..1744)
                     /gene="nepI"
                     /locus_tag="ACHOQV_000974"
     CDS             complement(554..1744)
                     /gene="nepI"
                     /locus_tag="ACHOQV_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFP9629388.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1955..2407)
                     /gene="yicN"
                     /locus_tag="ACHOQV_000975"
     CDS             complement(1955..2407)
                     /gene="yicN"
                     /locus_tag="ACHOQV_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFP9629389.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2460..3794)
                     /gene="adeQ"
                     /locus_tag="ACHOQV_000976"
                     /gene_synonym="yicO"
     CDS             complement(2460..3794)
                     /gene="adeQ"
                     /locus_tag="ACHOQV_000976"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFP9629390.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISSVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3969..5735
                     /gene="adeD"
                     /locus_tag="ACHOQV_000977"
     CDS             3969..5735
                     /gene="adeD"
                     /locus_tag="ACHOQV_000977"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP9629391.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNTYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTISRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVIRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5782..7173)
                     /gene="uhpT"
                     /locus_tag="ACHOQV_000978"
     CDS             complement(5782..7173)
                     /gene="uhpT"
                     /locus_tag="ACHOQV_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFP9629392.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQSFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7429..8748)
                     /gene="uhpC"
                     /locus_tag="ACHOQV_000979"
     CDS             complement(7429..8748)
                     /gene="uhpC"
                     /locus_tag="ACHOQV_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP9629393.1"
                     /translation="MLPFLKAPADAPLLTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGVSALLLLPFLNAQTPREA"
     gene            complement(8758..10260)
                     /gene="uhpB"
                     /locus_tag="ACHOQV_000980"
     CDS             complement(8758..10260)
                     /gene="uhpB"
                     /locus_tag="ACHOQV_000980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFP9629394.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10260..10850)
                     /gene="uhpA"
                     /locus_tag="ACHOQV_000981"
     CDS             complement(10260..10850)
                     /gene="uhpA"
                     /locus_tag="ACHOQV_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFP9629395.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10923..11213)
                     /gene="ilvN"
                     /locus_tag="ACHOQV_000982"
     CDS             complement(10923..11213)
                     /gene="ilvN"
                     /locus_tag="ACHOQV_000982"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP9629396.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11217..12905)
                     /gene="ilvB"
                     /locus_tag="ACHOQV_000983"
     CDS             complement(11217..12905)
                     /gene="ilvB"
                     /locus_tag="ACHOQV_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFP9629397.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAIAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLTHYGLINAVAACVDDSAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNETDPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(13011..13109)
                     /gene="ivbL"
                     /locus_tag="ACHOQV_000984"
     CDS             complement(13011..13109)
                     /gene="ivbL"
                     /locus_tag="ACHOQV_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFP9629398.1"
                     /translation="MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13673..13762
                     /gene="tisB"
                     /locus_tag="ACHOQV_000985"
     CDS             13673..13762
                     /gene="tisB"
                     /locus_tag="ACHOQV_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFP9629399.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(13886..13960)
                     /gene="ysdE"
                     /locus_tag="ACHOQV_000986"
     CDS             complement(13886..13960)
                     /gene="ysdE"
                     /locus_tag="ACHOQV_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFP9629400.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            14042..15226
                     /gene="emrD"
                     /locus_tag="ACHOQV_000987"
     CDS             14042..15226
                     /gene="emrD"
                     /locus_tag="ACHOQV_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFP9629401.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15234..15731)
                     /gene="yidF"
                     /locus_tag="ACHOQV_000988"
     CDS             complement(15234..15731)
                     /gene="yidF"
                     /locus_tag="ACHOQV_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP9629402.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15728..16090)
                     /gene="yidG"
                     /locus_tag="ACHOQV_000989"
     CDS             complement(15728..16090)
                     /gene="yidG"
                     /locus_tag="ACHOQV_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFP9629403.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(16080..16427)
                     /gene="yidH"
                     /locus_tag="ACHOQV_000990"
     CDS             complement(16080..16427)
                     /gene="yidH"
                     /locus_tag="ACHOQV_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFP9629404.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(16487..17980)
                     /gene="yidJ"
                     /locus_tag="ACHOQV_000991"
     CDS             complement(16487..17980)
                     /gene="yidJ"
                     /locus_tag="ACHOQV_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9629405.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAESIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKS
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGDNILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(17977..19692)
                     /gene="yidK"
                     /locus_tag="ACHOQV_000992"
     CDS             complement(17977..19692)
                     /gene="yidK"
                     /locus_tag="ACHOQV_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFP9629406.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     MGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            19859..20725
                     /gene="yidL"
                     /locus_tag="ACHOQV_000993"
     CDS             19859..20725
                     /gene="yidL"
                     /locus_tag="ACHOQV_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9629407.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(20815..22476)
                     /gene="yidE"
                     /locus_tag="ACHOQV_000994"
     CDS             complement(20815..22476)
                     /gene="yidE"
                     /locus_tag="ACHOQV_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFP9629408.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22672..23100)
                     /gene="ibpB"
                     /locus_tag="ACHOQV_000995"
     CDS             complement(22672..23100)
                     /gene="ibpB"
                     /locus_tag="ACHOQV_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFP9629409.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23212..23625)
                     /gene="ibpA"
                     /locus_tag="ACHOQV_000996"
     CDS             complement(23212..23625)
                     /gene="ibpA"
                     /locus_tag="ACHOQV_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFP9629410.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23619..23691)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23931..24263
                     /gene="yidQ"
                     /locus_tag="ACHOQV_000997"
     CDS             23931..24263
                     /gene="yidQ"
                     /locus_tag="ACHOQV_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP9629411.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24265..25479)
                     /gene="yidR"
                     /locus_tag="ACHOQV_000998"
     CDS             complement(24265..25479)
                     /gene="yidR"
                     /locus_tag="ACHOQV_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFP9629412.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHQLDSTLDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSNEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            25580..26644
                     /gene="cbrA"
                     /locus_tag="ACHOQV_000999"
     CDS             25580..26644
                     /gene="cbrA"
                     /locus_tag="ACHOQV_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFP9629413.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTMLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(26641..27978)
                     /gene="dgoT"
                     /locus_tag="ACHOQV_001000"
     CDS             complement(26641..27978)
                     /gene="dgoT"
                     /locus_tag="ACHOQV_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9629414.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(28053..29201)
                     /gene="dgoD"
                     /locus_tag="ACHOQV_001001"
     CDS             complement(28053..29201)
                     /gene="dgoD"
                     /locus_tag="ACHOQV_001001"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFP9629415.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(29198..29815)
                     /gene="dgoA"
                     /locus_tag="ACHOQV_001002"
     CDS             complement(29198..29815)
                     /gene="dgoA"
                     /locus_tag="ACHOQV_001002"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFP9629416.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEKSIPAIVDAYGDKALIGAGTVLKPEQVDALARMSCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29799..30677)
                     /gene="dgoK"
                     /locus_tag="ACHOQV_001003"
     CDS             complement(29799..30677)
                     /gene="dgoK"
                     /locus_tag="ACHOQV_001003"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFP9629417.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGFNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30674..31363)
                     /gene="dgoR"
                     /locus_tag="ACHOQV_001004"
     CDS             complement(30674..31363)
                     /gene="dgoR"
                     /locus_tag="ACHOQV_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFP9629418.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31640..32296
                     /locus_tag="ACHOQV_001005"
     CDS             31640..32296
                     /locus_tag="ACHOQV_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629419.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(32340..33152)
                     /gene="yidA"
                     /locus_tag="ACHOQV_001006"
     CDS             complement(32340..33152)
                     /gene="yidA"
                     /locus_tag="ACHOQV_001006"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP9629420.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTTLSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33267..33665)
                     /gene="yidB"
                     /locus_tag="ACHOQV_001007"
     CDS             complement(33267..33665)
                     /gene="yidB"
                     /locus_tag="ACHOQV_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFP9629421.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            33783..33884
                     /locus_tag="ACHOQV_001008"
                     /pseudo
     CDS             33783..33884
                     /locus_tag="ACHOQV_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33905..36319)
                     /gene="gyrB"
                     /locus_tag="ACHOQV_001009"
     CDS             complement(33905..36319)
                     /gene="gyrB"
                     /locus_tag="ACHOQV_001009"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP9629422.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36348..37421)
                     /gene="recF"
                     /locus_tag="ACHOQV_001010"
     CDS             complement(36348..37421)
                     /gene="recF"
                     /locus_tag="ACHOQV_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP9629423.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37421..38521)
                     /gene="dnaN"
                     /locus_tag="ACHOQV_001011"
     CDS             complement(37421..38521)
                     /gene="dnaN"
                     /locus_tag="ACHOQV_001011"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP9629424.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38526..39929)
                     /gene="dnaA"
                     /locus_tag="ACHOQV_001012"
     CDS             complement(38526..39929)
                     /gene="dnaA"
                     /locus_tag="ACHOQV_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP9629425.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40226..40306
                     /locus_tag="ACHOQV_001013"
     CDS             40226..40306
                     /locus_tag="ACHOQV_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629426.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40536..40676
                     /gene="rpmH"
                     /locus_tag="ACHOQV_001014"
     CDS             40536..40676
                     /gene="rpmH"
                     /locus_tag="ACHOQV_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP9629427.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40693..41052
                     /gene="rnpA"
                     /locus_tag="ACHOQV_001015"
     CDS             40693..41052
                     /gene="rnpA"
                     /locus_tag="ACHOQV_001015"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9629428.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            41016..41273
                     /gene="yidD"
                     /locus_tag="ACHOQV_001016"
     CDS             41016..41273
                     /gene="yidD"
                     /locus_tag="ACHOQV_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP9629429.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41276..42922
                     /gene="yidC"
                     /locus_tag="ACHOQV_001017"
     CDS             41276..42922
                     /gene="yidC"
                     /locus_tag="ACHOQV_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP9629430.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            43028..>44389
                     /gene="mnmE"
                     /locus_tag="ACHOQV_001018"
     CDS             43028..>44389
                     /gene="mnmE"
                     /locus_tag="ACHOQV_001018"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP9629431.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        10929 a        12036 c        11303 g        10121 t
ORIGIN      
        1 ttttatcctt ttattaatga ttacctgact atagcggtaa ggtcgctgca gtttaaaaaa
       61 cgaaacgcta tcgataagaa taaaaaggaa taaaagtgga atataaagtc tggcacttct
      121 tactgaccac gcaaacacct ttcgtacagc atgatgagag cggcaaaagc aaactacact
      181 tatgcttcat tcgatgcaca tttgcagaag gttgccgcta tgaagccaac gatgctactc
      241 atgattaccg tattcctgat ttttccagcc atttctcagg ccgagtcgcc attcagttct
      301 ctacagtcgg caaaagagaa aaccacggta ttgcaagatt tacgtaaaat ttgcacgcca
      361 caggcgtcat tatcagatga agcgtgggaa aagttaatgt tgtcagatga aaacaataaa
      421 caacatatac gcgaggccat cgtggcgatg gaacgtaata atcagagcaa ttattgggaa
      481 gcgttgggta aggtagaatg cccggatatg taagaacggc aacaaccact gaccaacgct
      541 tctttacctg atatcaggat ttcttcattt tcacctttgc agtcaccaac aatgcagtca
      601 gcaacatcaa tgtgccggac aacatcagcg gcgaagtcag accaatgtta tccaacgcat
      661 aaccgccgat tgccgcaccg caggtattgg caagctgaat aacggccacc tgaatagatc
      721 cagccttttc tgcctgatcg gccaatgagc gagtgatcca cgttgaccaa ccaacgggaa
      781 ttaaagcaaa ggtcagcccc cagataatcg ccacaccagt agcaacaatt ttatcgcttc
      841 cccacagcgt cagtaccagc gcgctcaggg cgagcacaaa cggtgcgccc gctaaggcca
      901 gtttcaccga acgtttgaga ataaacgacg aaagcgatgt cccgacaaag ctggcgatac
      961 caaaactcag cagcaccagg gttaagccat caacaccgaa tcccgccaga ttcatataca
     1021 ctgggcgaat ataagtgaaa aaagcaaact gtccggcaaa ggacataaag atggcgatca
     1081 tcccggccat cactcccgga cgttgtaata agcggaaagt attttgtttc tgatgcgaag
     1141 gttcacctgg cagcgaaggc agtgatttga taatccagaa aatacacagc acacccatcg
     1201 ccgccgccgc attaaagaca ttgcgccaac cgataagctc gcctaaaaaa ctgcccaacg
     1261 gcgcggcaat caccagcgca atagaaaccg cgccgaagat caccgacagc gccttcggca
     1321 ccgtacgcgg cggtaccaga cgcatggtca gcgacgccga catcgcccag aacccgccca
     1381 gcgccagccc cagacaggcg cgaccgatta acagcagact gaatgagttg gcaaaggaaa
     1441 ccagcaaaca ggaaagcgtc agcaaaacgg cgaacagaat gacaacgtag cggcggtcag
     1501 tggcctgaat ggtctgggta ataaacaaac tggcaaacat cgccacaaag gcggtcacgg
     1561 tcaccgattg cccggcaacc ccttccgaaa tgcctaaatc ctgggccatt ggcgtcaaca
     1621 aactgacagg caaaaactca acgataatca gacaggcgac gcaaaacgcc accgagaaaa
     1681 cggctgacca gttcggtcgg gtgatggcat ccgcgccgcg gttttcagca ataaattcac
     1741 tcatggtgtt acccgtggca tgtttcagag ggaaagccga ccactttaac attgaaatgt
     1801 gtgacgtatt taacgttttt gcaactttta cggtgttgct tgagctggag ttaagatcga
     1861 aacggaacag ggcaaagatt accggatgcg gcgtgaacgc cttatccacc ctacaggtca
     1921 ggcgcggtct gtaggctgat aagacgaagc ggttttaatg cagccagtga acgccctcgg
     1981 caggctgaaa gaacgcgtta tagcgcatct gaaaagcatt aatatcctgc atttcaggtt
     2041 cggtttcgcg cagaaaaccc agcgccagcc tgacctgagc atcggtaatg ggagcggcaa
     2101 ggcgggcttt ttgtaaaatc cgatgccatt gcagcaagtt ctgttcgcta ccatcgacaa
     2161 tgacaatttg ccagatgagc aacacttgcg cggcgttttg caggtgcgac tcatccggac
     2221 gatgcaaata acgtaaaaat tcgtcaccgg ctgactttcc catttgatcg accatcgact
     2281 ccacgggtac gacatcgatg ttcagccgat cgctgagacg gcgaatcgct tttctggccc
     2341 cggatgtcag cacccgaaaa ccaaccacaa acactaccgc cagcgtggcg agcattatcc
     2401 agatcatgag ttctcctgcg taatcagaac tcatgatatc tggaaaccct cgccagttac
     2461 taatccacca gaataatctt cagtgcaaac agagctgcga ccaccaccac acacaggttc
     2521 agatcgcgcc agcgcccggt gcacactttc atgatgcagt acgacataaa gccgagtgca
     2581 atcccttcgg tgatcgagaa agtaaagggc atcatcaccg tggtaataaa cgcaggtacc
     2641 gattcggtaa aatcatccca gttaacgcgc gccagacttg aggtcatcaa cacgccaaca
     2701 aagattaacg ctccggcggt tgcgtaagga ggaaccatcg ccaccagcgg tgagaagaac
     2761 ataaccaaca ggaacataac gccgaccaca accgcagtca gccccgtgcg accaccgact
     2821 gccacaccag aagtactttc aatataggcg gtaacagacg aggtgccgat aaacgcaccc
     2881 gccaccgaac tgacgctatc aacatacagc gccttattca tattagggaa tttaccgtta
     2941 ctatctatta agccagcttt atcagttaca ccaattaatg ttcctgatga atcaaatagg
     3001 ttgatcagca taaaggagaa aatgataccg gcgagttcaa gtgataacgc gccgctcaaa
     3061 tccacttcac caatgacgct gctaatatca ggcggaatgg aatagacgcc gctaaaatga
     3121 acatcaccga aaaataatcc acagcaagac gtcaccacaa tagaaacaag caccgcggca
     3181 tgaaaatgac gtgatgacaa cacggttata ataaaaaacc ctaaaatacc taacaacacg
     3241 ccgtgagaac ttaaatcgcc aatcatcacc agggtgtctt tattggcgac aataacgcca
     3301 gtatttttta atcccattaa ggcgataaat aatccgattc cgctggtgat accaatacgt
     3361 aaacttaatg gaatgttgga gatcatccag taccggatac gaaacagcgt gagcagaaat
     3421 agcccaactg cgccccagaa tatcgcgccc atcccggtct gccaggagat gcccatcgcc
     3481 cccacgacca cgaacgcaaa gaaggcgttc agccccattg ccggagccag cgccacgggt
     3541 aagttagcaa atatccccat cgcaatactg ccgataccgg caatcaaaca ggtggtaaca
     3601 aacaccactt tcgggtccat ttgtgccgcg ccgaggattt gcggattcac aaaaacgatg
     3661 tataccatgg ttaaaaaagt ggtcatcccc gcaatcaatt ctgtgcggac ggtggtccca
     3721 tgctgaggta gtttaaataa tcgcgaaagc gtccctgatt cattactcac gtaatcggta
     3781 ttgtcattat tcattttttt gtccattgga ggagatttaa tcccttgctt ttgtaaaaca
     3841 aatgacaaca acggttcagt gataattatc acatttcaat tgcacattaa tggatattct
     3901 ttaataatct cgcgacgttt ctttatgata aataataatc aaattgataa aatcaaaatg
     3961 agaaaaatat gaataattct attaaccata aatttcatca cattagccgg gctgaatacc
     4021 aggaattgtt agccgtttcc cgtggagacg ctgttgccga ttatattatt gataatgtct
     4081 ctattctcga cctgatcaat ggcggagaaa tttccggccc aattgtgatt aaaggacgtt
     4141 acattgccgg tgttggcgca gaatacgctg atgctccggc tttgcagcgg attgatgccc
     4201 gcggcgcaac ggcggtgcca gggtttattg atgcgcacct gcatattgaa tccagcatga
     4261 tgacgccggt cacctttgaa accgctaccc tgccgcgcgg cctgacgacc gttatttgcg
     4321 accctcatga aatcgtcaac gtgatgggcg aagccggttt cgcctggttt gcccgctgtg
     4381 ccgaacaggc aaggcaaaac cagtacttac aggtcagctc ttgcgtaccc gccctggaag
     4441 gctgcgatgt taacggtgcc agttttaccc ttgaacagat gctcgcctgg cgggaccatc
     4501 cgcaggttac cggccttgca gaaatgatgg actatcctgg cgtaattagc gggcagaatg
     4561 cgctgctcga taaactggac gcatttcgcc acctgacgct ggacggtcac tgcccgggtt
     4621 tgggtggtaa agaacttaac acctatattg ctgcgggtat tgaaaactgc cacgaaagtt
     4681 atcagctgga agaaggacgc cggaaattac aactcggtat gtcgttgatg atccgcgaag
     4741 ggtccgcagc ccgcaatctc aacgcgctgg caccgttgat caacgaattt aacagcccgc
     4801 aatgcatgct ctgtaccgat gaccgtaacc cgtgggagat cgcccatgaa ggacatatcg
     4861 atgccttaat tcgccgcctg atcgaacaac acaatgtgcc gctgcatgtg gcatatcgcg
     4921 tcgccagctg gtcgacggcg cgccactttg gtctgaatca cctcggctta ctggcacccg
     4981 gtaagcaggc cgatatcgtc ctgttgagcg atgcgcgtaa ggtcacggtg caacaggtac
     5041 tggtgaaagg cgagccgatt gatgcgcaaa ccttacaggc ggaagagtcg gcgagactgg
     5101 cacaatccgc cccgccatat ggcaatacca tttcccgcca gccagtttcc gccagcgact
     5161 ttgccctgca atttaccccc ggaaaacgct atcgcgttat tgaggtcatc cataacgaat
     5221 tgattaccca ctcccgctcc agcgtctaca gcgaaaatgg ttttgatcgc gatgatgtgt
     5281 gctttattgc cgtacttgag cgttacgggc aacggctggc tccggcctgt ggtttgctcg
     5341 gcggctttgg cctgaatgaa ggtgcgctgg cggcgacggt cagccatgac agccataata
     5401 ttgtggtgat cggtcgtagc gcagaagaga tggcgctggc ggtcaatcag gtgattcagg
     5461 atggcggcgg gctgtgcgtg atccgtaacg gtcaggtaca aagtcatcta ccgttgccca
     5521 ttgccgggct aatgagcacc gacacggcgc agtcactggc ggagcaaatt gacgccttga
     5581 aagccgccgc ccgtgaatgc ggtccgttac cggatgagcc gtttattcag atggcgtttc
     5641 tttccctgcc agtgatcccc gcgctgaaac taaccagtca ggggctgttt gatggcgaga
     5701 agtttgcctt cactacactg gaagtcacgg aataataaaa aaaagcccgg cgtcatgccg
     5761 ggcaaaagtc accagttaag tttatgccac tgtcaactgc tgaatttttt tctcgcggcg
     5821 gattttgcgt tcttccatca ccgccactat cgccatcagg cagatacaac caatcgcggc
     5881 gatatccagc gcggcgaagg tgcctgccca gccggtaagg ccaaataccg gcgtcccatc
     5941 ggcaatcatt cccagaccta acttggcaaa gctgtcacca atcaggtaag cgaaggtgcc
     6001 tttaatacca tcggcagcgc caatcgcttt tttaggtaca aagccaacag cagccacacc
     6061 aatcaacaat tgcgggccaa agaccaggaa acccaacgca aagagagaag ccaggtagat
     6121 atattcgttg ctggcgtgtt gatacacacc gagcgtggcg ataatcagcg ccagcgcgat
     6181 acaggccacc aggccacggc gaccgttcgc caggtcagag agccagcccc acagcagcgt
     6241 accgaccagc gcaccagctt caaacagcgt aaagctctga atcgccaccg ctttagagag
     6301 tttcagttcc tggaacgcat acacggttga ccactggtcg ataccaatac gcaccacata
     6361 gaggaaaatg ttggcgaagc acagcagcca gatcactttg tttttcagca catactcaac
     6421 aaagatctgc cacttggtca tatcggtaga ttctgtctct ttgtcctctt cgctgatctc
     6481 ctcgccgaac agctcttcag ctttgccgag gccataagat tccggggagt cgctgccgta
     6541 acgcaggccg ataaaaccga caatcagcgc gataatcgac gggaaaataa acatgccgat
     6601 gacatgacca tcgaacaggt aatttgcgcc gaacagcgcc acacctgctg cgcctgcacc
     6661 accaaggttg tgagaaatgt tccagaaacc gaggaatgtc ccgcgtttac gacgcggcgt
     6721 ccatttggtg atggtggagt aactgcacga accgccggta ctctggaaaa agccgctcaa
     6781 ggcgtagaag gcaatcatca ggaacaggct aaccgagccg ctgcccatac tggcactgaa
     6841 gcccagcata caaatggcgg acaggatcag catgaacggc aggaattgtt tggtgttttt
     6901 gccgtcggcg tagtaagaaa ccagcgtttt acccacgcca taagtgatgg agaagcccag
     6961 gccgatcatc cccagctgcg tcatgctcaa cccgtaggtc gagatcatgt cgttctgcgc
     7021 gatgttaaag ttcttgcgaa tcaggtacat cgtaaggtag ccgataaaga ccaccaggta
     7081 ggattgcatg aacggtttga accacatttt gcgccgcact tcgagcggaa ggtccagggt
     7141 cggcttgcga acctggttta ggaaagccag catgggttac tcctgatttc ccttcatact
     7201 tcaagccgca ggtgccttag ctgcacgcat tcctcccggt cacatagttc actatgctcc
     7261 cgggactcac gcgtttgctg cgttcctgca acttgaatta ttttgggaaa gaattaatga
     7321 atgatggcat tgtaaaaatc agcagcaaat caggcctgag atagcgtcca ggcaaaacct
     7381 ggaaaattgc ttagttttgc cggatatgaa gtgaaaaggt gagatgcatc acgcttcgcg
     7441 cggtgtctgg gcgttcaaaa agggcagtaa cagcagtgcg gaaaccccgg cggcaatggc
     7501 gataaccaca aaaaatccgc tccagtgcca ggtatcgagc actttcgcca gcggccaacc
     7561 agcaagcgac gcccccagat aagcaaacaa gccgacaaac cccgtcgccg cccctgccgc
     7621 ctctttgtgg gaacattctg ccgccgccat gccgattaac atctgcgggc caaagacaaa
     7681 aaaaccaatg gtgaagaagc aggttgcctg catcacgtag ctggcaaatg gcatcagcca
     7741 cagcgagccg actgaaagca aaattccggc ggcgaaaatc aaattcatcg gcccgcggtt
     7801 gccgttaaac aatttgtccg agccccaacc cgctaccagc gcaccgataa atccacccag
     7861 ttcaaacatc gtcactgccg tattcgccgt gaccagatcg acgcccagcg tctcggacat
     7921 atacaaattg ccccagtcgt tgattgccgc ccggaccaca tagaccagca catagcaaaa
     7981 cgaaagcagc cagatatacg gattcagtaa cacatatttg gtgaggatct ctttacgcgt
     8041 cagccctgcc ccttcttgtt gttgagctat ctccagcgcg tcgtgccgcc attcaccaac
     8101 cgccggtaaa cctaacgcct gcgggcgatc gcgcagccgc cagcagagaa aaatccccac
     8161 gactatcgcc atacaacccg caatcatcat cccggcacgc cagccgtaat gcagcgcgct
     8221 cgctgccatc acaatgggaa tgagtgcgcc gccgacgtta tgcgccgtgt tccataatgc
     8281 ccaccaaccg ccgcgctcgg tacgagaata ccaggcggtt aacagacgcg cacacaccgg
     8341 tgaaccccag ccctggaaaa aggcgttcaa cacccaaagc acggcaaacg cccatagcga
     8401 cgtcgagaag ccaaacagaa tgttgataat gcccgtggca ataagcccta tccccataaa
     8461 ataacgggca tttgagcgat cgctgacaat gccggagaca aacttcgaca cgccgtaggt
     8521 aatgtaaaac agggtcgcta acaggccaat atcgctacgg ctgagcacgc cgttagcaag
     8581 gatttctggt acggcggcgt taaaactttt acgcgtgaag taaaacagcg cgtaacccag
     8641 ccagatggtc agcagaatat gccgacgcca gtagcgatag cgggcatcaa tttcatgttt
     8701 atcagtcagt aatggcgcat cggcaggcgc tttcagaaac ggcaacatca tcgaacctta
     8761 gacataacgt tgaggtagag aaacgctgac acgcgtgccg tgcagacagg aaatgtgtaa
     8821 tgtgccaccc agcgccgtta cgcgctcgcg cattccggta aggccaaaac cgtgttgccc
     8881 ggaatctggc ggtaaaccgc tgccatcgtc ttcaatcacc agcattaacc gctcatcatg
     8941 ctgccagcct tgcagcgtga ccgcgctggc atcggcatgt ttcacaatgt tgttcaaccc
     9001 ttcctggcag acgcgaaata gcgtcacgcg ctggttttcg cttaacgctg attcatcgat
     9061 tcgccattcg agatgactga caatgccgcg cccttccagc tccatttccc gcatcagtga
     9121 gcggatggcc tgctccaggg tgagatcatc cagctggcgc ggacgtaacc gccctaacaa
     9181 gcggcgcacc gcgtcgtaaa cgcccagcga tagttgttcg atgagctgcc cgctctgttt
     9241 cacgctggcg ttatccgccg cgagccgctg gacaatgccc gcttgagttc gaatagcagt
     9301 aatggtctga ccgatatcat cgtgcagctc acgtgccaca tcacggcgca cgctctcttc
     9361 ggtttccagc aaccgttcag cgagatgctg attgcgcgcc agttcctttt gcagcgactg
     9421 gttaagttca cgcaaccgct ggatgcctgc gcccagtaac aaccctgtca gactttgcac
     9481 cagcagcgag agcaataaat ccaccggatg atcgcgccag gtttgactgg cgatcagcgc
     9541 gatggcgttc atcaacgtcg caattagcgc cccttgccag ccatagtgcc aggccagcgc
     9601 gataatcggc agcgccaggc agaatggcgt aaagcgcgac agttcgtccg gcaatcccaa
     9661 ctggagccag agactgataa caaacagcag caagtaccag accagatgcc gcccacgcca
     9721 gttgatcggt tgagaaacta acgacgggcc gagcggcagc caggtgttat tggcgagata
     9781 gtgccagaac accagacata tcggggccag cgtcaggccg ccagttaaag tcagcaacag
     9841 cgcattccac gactctttgc cgtgccaaag ccagggcagc gactgcaaca acgccgccgc
     9901 cgttaatgcc gccccttgta gcagcaaggt gcgccagtca cgttgatgtc gatagcgcga
     9961 gatcagcgcc acgggcagta acgtcagtaa actaccgatc atcaataagg agaaatgggt
    10021 taaaccgacc gcctgcgtta accagtaaat cagcagccac tccgcgccca gcaacaccgg
    10081 ccagtatccg cgcgggcatt gcagcattag ccccagacgc agaccaaacg gaaataacag
    10141 caccgccata tcagggcgct caaccaggtg caggctgata ctccacaggc aaaaccatgc
    10201 ggcagagaag ataaaaaagc aggcaataac ggtaattaag cgggagaaca acgtcttcat
    10261 caccaaccat caaacatgcg gcgcgccagt tcaacgtcgt tactgacgcc cagtttttcc
    10321 atcagattgg cgcgatggac gtgtaccgtt ttcggcgaca agcccagttc ggcggcaatc
    10381 tctttcaccg ccattccttg cgccagtttt tctgccacct ggcgttcgcg tttggttagc
    10441 gggtcctgac gaccggatgc cagtttaatg gcaatatccg gcgtcagata acagccgccc
    10501 gtggcaaccg tatgcaccgc agcaatcagt tcgtcagggc tacaacgctt ggagagaaag
    10561 ccgcgcgccc ccgcgttaag cgcctgctca accagcgccg gactgtcatg aacagagagc
    10621 ataatcgtcg ccataccttt cggcagctgg cttagcagct ccagaccgga gatatcgggc
    10681 atggagatat cgcaaataca cacctgcaca ccgcgccccg gcaaccccgc cagcgcctcg
    10741 cgccccgaac caaactcggc aactacctgc aaatcaggtt ccagccccag cagctgcgca
    10801 aagccggagc ggacgataag gtgatcgtct ataagggcaa cggtgatcat gatcttgtcc
    10861 tggcgggtaa taaaacgcgc ttaccttaac gataagcgcg atgttgttca agccttgagc
    10921 ggttactgaa aaaacaccgc aatcttgtta aacattgtcg gatcggactg attacgctgc
    10981 actttcacca catcttccag cttatcgatt tggcttatca tctgctccag acgctggtcg
    11041 tcattgacca gtagccagat atggcttttg tcgctgtcct gaatcggcag gcagagaatg
    11101 ccttcaacgt taaaagcgcg acgggcaaaa aggccacaaa cgtgggtcat tacgcccgga
    11161 tggttgcgaa cggtgagttc gagaatcacg ttgtcatgag ttgtgttttg catggcttat
    11221 tcccccacca tttcagtatt cgccgcacct ggcggcacca tcgggtacac tttttcttcg
    11281 gcatcaatgc gcacgtggat cagcgccgga ccagggcgat tgatgatttc ctgcaatgca
    11341 gcctgcggat cggtttcgtt attcaaatca caggtttcga ggccgaatcc ggcggcaatc
    11401 tgcataaagt tgatttttcc cggataggta gcggcaaaaa cgccctgctt gtagaacaga
    11461 ctctgttgct gatgtaccag ccccagcgct tcgttgttca tcagaatgat tttgacatcc
    11521 agctgatttt cgctagcggt cgccatctcc tgaatattca tcatcaggct gccgtcgccg
    11581 gagaaacaca acactttgcg atccgggttc gcaagcgctg cgccaatcgc cgcaggcagg
    11641 ccaaagccca tcgtgcccag cccaccggag gtcagccact ggcgtgggcg attgagcgga
    11701 taagcctgcg ccgtccacat ctgatgctga cccacgtcgg tggtgataat cgcgctgtca
    11761 tcgacacagg cggcaacagc gttgatcagg ccgtaatgcg ttaacggatc gcacgctttc
    11821 gggattggac acgggaattc acgctgcaaa tccgccacca actggtgcca ctctgcacgc
    11881 ggttgcgctt ccaccagcgg gatcaactgc gccaacacgt catcaacatc cgcctgaatt
    11941 gccacatgcg gttgcttgat tttgcccagc tctgcgcggt cgatatcgac atgaataatt
    12001 ttggcattcg ggcagaactg ctcggttttg ccaatcgccc ggtcatcaaa acgcgcgccg
    12061 agaacaatca gcagatccgc ctcctgcaaa atatagttgg tgctgcgcac gccgtgcatc
    12121 cctagcatac ccagcgacag cggatgcgct tttggcaaca tacccagcgc cattaatgtc
    12181 attgtggtag gcagctgcgc tttctccgcc agttcacgca ctctcgcagg cgcattgatc
    12241 acaccaccgc ccagataaag caccgggcgt ttggcagcat taatcattgc cgctgcgtca
    12301 cgaatgcttt cttcgctaaa ggcgggcgcg atggcttttt ctgccaccgc gggctgtgct
    12361 tcaatctcaa aaacggccgt ttgcacatcc ttaggaatgt ctatccacac cgggccaggg
    12421 cggcctgatt gcgcaatacg gaaggcatcg ctcatgacct gcgggagttc ttcgatatgt
    12481 ctgaccagat agttgtgttt ggtgatgggg atagagatgc cgtaggtgtc gacttcctgg
    12541 aaggcgtcgg tgccgatcat cgaggcggga acctgaccag tgatgcaaat cagcgggatg
    12601 gagtccagcc gcgcatcggc aatggcggtc accaggttag tcgcccccgg tccgctacag
    12661 gccatacaga ccgccggttt accgtcggtg cgcgccattc cctgagcgat aaaccccgcg
    12721 ccctgttcat ggcgagccag aatatggcgg atttgcgtgc tttggcttaa ggcatcgtaa
    12781 acaggcagga tagaaccgcc cggaatgccc gtcacaatct taatgccctg ctgttccagg
    12841 aaatgaacga taaattctgc gccggtaaag cgcttacgcg tcgatgttgt gcccgaactt
    12901 gccatgctcc agtccttttc ttctgggccg actttccggg aggtgcttaa acgaaaaacc
    12961 ccgcccggtt tgcgccggcg gggttttgga atcgtgtgtt gttccagtcc ctacggcgca
    13021 ttgccgacga ccaccaccac acgcacgacg accactgcgg cggatggcgc agttggtagt
    13081 agttttgcgt tgagcatgga agtagtcatt ggggaccttg ttggttttgt gtttaacaat
    13141 atttatacaa gcacagcttt acaggggaga caatggaaaa tttttcagca agggaaaatt
    13201 gaggggttga tcacgttttg tactgaattg caggtaacaa aaaaccccgc cggagcgagg
    13261 tttcgtcagt cgcctgcggc tggtaaccgc aaagcacact gtattatgtc aacactgaaa
    13321 gtatacgtgt tccgcgcaga acgcgcaatt tcggcacgaa ttttgacgta tttagtgcat
    13381 agttgagtat cgatcacagt ttgcgttttg tccaaaatta ctgtttattt atacagtaaa
    13441 cttctataat atcactgtac gcaatgtgtt atgcgggggc cgcatcgtta cccggcgcac
    13501 taagtcctgg cagaaacggg tggtgccgtc agcgccttaa ccccgcgtga gcacactgtg
    13561 ttatgtcaac aagcacaacg tttctccttg agataccgcg tgcacaacag ctggcaacag
    13621 gcagcggaaa ggtacgtcag ctggcagtgc tcctgaacca caggagacgc gtatgagcct
    13681 ggtggatatc gccattctta tcctcaaact cattgttgca gcactgcaac tgcttgatgc
    13741 tgttctgaaa tacctgaagt aattcagatt caagtcgcac taaaggggag cgggtaaccg
    13801 ctcccctttt atatttagcg tgcgggttgg tgtcggatgc gatgctgacg catcttatcc
    13861 gccctaccat ctctcccggc aacatttatt gccgcttttg tttacatatt ctgccgctaa
    13921 acaattcccc attcctggcg tatatctggc taacattcat caatgtgata gattcctctc
    13981 ccgcatttat gggaatgcgt agtgacttat tctaattatt tttataaaag catccgtgat
    14041 aatgaaaagg catagaaacg tcaatttgtt attgatgttg gtattactcg tggccgtcgg
    14101 tcagatggcg caaaccattt atattccagc tattgccgat atggcgcgcg atctcaacgt
    14161 ccgtgaaggg gcggtgcaga gcgtaatggg cgcttatctg ctgacttacg gtgtctcaca
    14221 gctgttttat ggcccgattt ccgaccgcgt gggtcgccga ccggtgatcc tcgtcggaat
    14281 gtccattttt atgctggcaa cgctggtcgc ggtcacgacc tccagtttga cggtattgat
    14341 tgccgccagc gcgatgcagg ggatgggcac cggcgttggc ggcgtaatgg cgcgtacttt
    14401 gccgcgcgat ttatatgaac ggacacagtt gcgccatgct aacagcctgt taaacatggg
    14461 gattcttgtc agtccgttgc tcgcaccgct aatcggcggt ctgctggata cgatgtggaa
    14521 ctggcgtgcc tgttatctct ttttgttggt tctttgtgcc ggtgtgactt tcagtatggc
    14581 ccgctggatg ccggaaacgc gtccggtcga cgcaccgcgc acgcgcctgc ttaccagtta
    14641 taaaacgctt ttcggtaaca gcggttttaa ctgttatttg ctgatgctga ttggcggtct
    14701 ggccggaatt gccgcctttg aagcctgctc cggcgtgctg atgggcgcgg tgttagggct
    14761 gagcagtatg acggtcagta ttttgtttat tctgccgatt ccggcggcat tttttggcgc
    14821 atggtttgcc ggacgtccca ataaacgctt ctcaacgttg atgtggcagt cggttatctg
    14881 ttgcctgctg gctggcttgc tgatgtggat ccccgactgg tttggcgtga tgaatgtctg
    14941 gacgctgctc attcccgccg cgctgttctt tttcggtgcc gggatgctgt ttccactggc
    15001 taccagcggc gcgatggagc cgttcccctt cctggcgggc accgctggcg cgctggtcgg
    15061 cggtctgcaa aacattggtt ccggcgtgct ggcgtcgctc tctgcgatgt tgccgcaaac
    15121 cggtcagggc agcctgggat tgttgatgac cttaatggga ttgttgatcg tgctgtgctg
    15181 gctaccgctg gcgacgcgga tgtcgcatca ggggcagcct gtttaagctc acgtcaccgc
    15241 agcatcgtca tcagctccat gggagaacga tgctgcttta tcagatcacg catcacccgc
    15301 atatgcggcg cggagtaaga ataaaacgcc tgatagcccg cacaaagcac gttgtctggt
    15361 ttatcttccc gagcgtgcgg gcacagccga tgccagtcgc acgcctggca gtcacggcgc
    15421 ggttgcgact tttcgctgcc acaccatttt tgtaaggtgg tgtcaaaaag ttcaatcgaa
    15481 atacgactga tatctgcccg cacccaacac tcgaacaggg caattaaaaa tttcccccac
    15541 agctctgctg tcaccgaagc ggccgtcaga tcgccccggt catcaaattc aagatgggga
    15601 ataagctgta ctgagcggat gcccagctgt ttgaaattgt gataaaaatc ggcaggttgc
    15661 agagcatcct tatactctac aaccagttta tgcctgtcct cttcagcatc aatgacctga
    15721 cttcctgtca tgcgactctc tcaataaata cgataagttg atgtatatgc gttacagcaa
    15781 acagtattgc gagagataac accgcgaggg agatcaaaaa tttgtcacgg actacgtgaa
    15841 attgggaaaa atcgctattc gtgacatcca ttaaattgcg attacgggtg tagtgccaga
    15901 ggatcagcgc cacgatggcg aggatgccaa tcgaaatcca gaataacatg cccgcctggt
    15961 gccagttgtg tttgatagcc aacgccatca acgcgccgta gcccagcatg gtacgaaacc
    16021 acgccagtga tgtgcgttcc ggctgcagcc ctgggtcggc aatgcgtctg gctttgcggc
    16081 tatccggcat acaacaccag tcccatgacg atcactgcca cgaccatcaa tattaaactg
    16141 atgattaata agctgttggt atagggcagg tcctctttca ggcgcatcgc tttttcattg
    16201 cgtaaccagc gcaagtagcc atacatcgct aacccgccgg aaaacagaca caacaacaac
    16261 gccagcagtt cgcggatgac gggcgtggcg aaatctggcg caagttgatc aagcccaacc
    16321 cccgccgcca gaaatcctag tgcagtacgg atccacgcca gaaaagtacg ctcattagcc
    16381 agcgagaagc ggtaatcggg cgcttctccg aggcgggaaa ttttcattgt tgctccttgt
    16441 aaactgttcc tcgccgaaaa attaggccag gaacagcaat aatgcatcag aacttctgtt
    16501 ttttctcttc caccttcacc ccttgtgtcg gtaaaccggt gtcatagtcg cgtaccactg
    16561 gagaataacc atcctgtggg cgtggacgaa acgcccccat ccagcgcggt tgtgcatctt
    16621 tgcgccacgg acgcaggttc cattgataac tgcggaacgg atcgcgaatt ttgtccatat
    16681 aatccaataa ggcgtcatgc attttgctgc gaacgtcggc aaaatggata tcatcgatca
    16741 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    16801 tgagcaccag tttaaagtca tccgttaccc agcaacgcac cggaataaaa ccgccaaagc
    16861 tgtcatgctc aatctcgtag cgattaaatt ccaccaacac gccacgtggt tcttttacgg
    16921 caaggatatt atcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    16981 tcggcagtaa gtcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17041 aacggatgat cagcggaatg cgggtgatgt cgtcatacat cgccgcccct ttactgatca
    17101 gcttatgtgc gcccatcatt tcaccatgat cggaggtata aatgacccac gtattttcac
    17161 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17221 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17281 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17341 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17401 gcggctcatc atacgaaacc accatcagga aaggttcgtc ggcgcgcgcg ggctgttgca
    17461 gaaaatctac cgcccgattg ctgatgcggt gcgcccaggt gaaggtttcg tcgatatggt
    17521 tcgcctgtaa atcctcaacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17581 tcagttcgct aaggtagttc gccccatcga accagtaatc agcgtcccac tccggcggac
    17641 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccaatg taacaggtgt
    17701 gatagcccgc atccttaaag tagcgcccca tggtggaaat gtttttacct ggcgcgacgt
    17761 tgttggtcca cgggccggac tggttagcgt agataccggt aaacagcccg gcgcgcgctg
    17821 gtgtacaaac cggtgaacag gtataggcgg aattaaagcg aatactttcc gccgccagac
    17881 tatcaatatt ttgcgtattc agcggcttac cgctatagca accgaccata ttggtggcct
    17941 gggtatcggt catgatgaac agaaaattgg ggcgtttcat aggctatcct tcgcgtcggg
    18001 agtaaaggtt acggctgggt cgtgacgatg ccgccagcta tcaaaaatca ggtagacaat
    18061 cactacggcg gcaatgaaat aactgatcat cgctaaccag cgcgtaccgt agccgccgaa
    18121 ttcagccagc ccggcataga cgccaatcat cgcgaacagg atgcccattg acgcgatctt
    18181 gacgtttttc cacggtttca tgtccaccgc aaacgcatct ttgaaggtga acggcgtggc
    18241 gcgcggtttg ataaaaccga tcaccagcat cacgaccacg ttgatgcaga acgtacaggc
    18301 cagcacatag aggaaatgga agtcgaactt caccagatag ttgatggtga tgtagctgat
    18361 tatgccaatc cccatcgcca cttttgccgc cagcgccggg atgcgcggga agaaaaagcc
    18421 cataatgatg atggtaacca gcggcacgtt gtaaatgccg ttgagctgtt tcatccagct
    18481 atacagcccc tgcggcgcgt tggcgatcca cggcgctacc agcaccgaaa cgatggcgat
    18541 aaagaaacca aatttgcgcc cgacggtgac cagctgctgc ggctcggcat tctggttaat
    18601 gatgcgccgg taaataccca tactgaataa cgtactggcg ctattcagaa agccgttgaa
    18661 ggtactgatc accgcaccaa ataacaccgc gccgaagaaa cccaccagtg gcactggcag
    18721 aacgttattg accagcgtcg ggtaggccat gtcggctttc ggtagatcct gatacagatg
    18781 aaacgcaatc aaccctggca gcaccagtac cagcgggtcg agcattttca gcaccgccgt
    18841 taacagcgcc cccttttgcc cttccgccag gctttttgac gccagcgtgc gttgcacgat
    18901 gccctgattt gtacaccagt aaaaggtgtt caccagaatc aaaccggtaa atgccgcgcc
    18961 aatcggcaag ggatcggtcg ggccgccgac tgagtttaat ttctcggcgt gaacggtggt
    19021 gagttgctca atgccctgca taaagctgcc cttgcccatc gcgatcaggc caaataccgg
    19081 caccatcaac ccgccaataa ccagcccaat accgttaatg gagtccgcca ctgccattgc
    19141 gcgcagtccg ccgatcaccg catacaaaat tcccgccaga ccaagcaaaa taaccagtaa
    19201 ccagatagcc gcaccatcgg aaagttgtag cgactcaccc acatgaaaca ggctgttgag
    19261 cgccaacgcg ccggaataga gaacaatcgg cagaaagcag acgccggtgg caatgaggaa
    19321 gcagaagtcg atgataatac gcgtcgtttt atcataacgt tcctccagaa aatcggggat
    19381 ggtggcaatg ccgcgcttca gatagcgcgg taaaaagatt agcgcgagga agatcagcgt
    19441 cactgcagag gtgacttccc agcccatcac cgacatgcca cttttgtagg cctgcccaga
    19501 aaggccgacc agttgttccg tggaaaggtt ggttagcatt aacgaagcgg caataaccgg
    19561 cgcttttagt gaacgtccgg caagaaaata gccttgttgc gatccggtat ctgttttacg
    19621 gaccttccac caggtgatga ccgctaccag cagcgtaaaa ccgacaaaac tcaagatttg
    19681 taacgaattc atctcatagc ccttatttat gtttatgtcc cggacgcatg aatataggga
    19741 gaggtaaaat gcgtcgggtc aggcagtcag attcttaaaa tacaaacgtc gtatccctga
    19801 acggattgtt ttctgttaag ttcaggttgt gagcataatt cgccggaggg atattaaaat
    19861 gaatggaaaa ttgcaaagtt cggatgtaaa aaacgaaact ccgtacaata ttcccttatt
    19921 gattaacgaa aatgtgatct ccagcggaat ttctctgata tcgctctggc atacctacgc
    19981 cgacgagcat tatcgggtga tctggccgcg ggacaagaag aaaccgctta tcgccaactc
    20041 atgggttgcg gtgtataccg tacaaggatg tgggaaaatt cttttaaaga atgacgaaca
    20101 aataactctg catggcaact gtattatatt tttaaagcca atggatattc actcttatca
    20161 ctgtgaaggt ttggtctggg aacagtattg gatggaattt acccccacca gtatgatgga
    20221 tattcccgtc ggtcagcaaa gcgttattta taatggcgaa gtttataatc aggaactcac
    20281 cgaagttgct gagttaatta cttcaccaga agcaataaaa aataatctgg cagtcgcttt
    20341 tctgacgaaa attatttatc agtggatttg tcttatgcac gcagtcggta aaaaagatcc
    20401 acaacggcgg caaattgaaa aattaattgc cactttacat gccagtctgc aacaacgctg
    20461 gagcgtagct gatatggctg ccacgatccc ctgtagcgag gcctggttgc gtcgtctgtt
    20521 tttacgctat accggcaaga cgccgaaaga atattacctc gatgcgcgtc tggatctggc
    20581 gctatcgcta ttaaagcaac aagggaactc ggttggcgaa gtcgctgata cgctcaactt
    20641 cttcgactcc tttcatttca gcaaagcctt taagcataaa tttggttatg cgccgtcagc
    20701 tgtgctgaag aatacggaca ggtaataacc agaatggcga gtgtgtaaat gactcgatga
    20761 tgttcagaaa taccaggtgg agtaccattt acggcgactc cacccggaaa agagttaacc
    20821 gatactccag aagagcaccg ccagtaattg gggggtgata atgcgcagga acatcaccaa
    20881 cggatagaca gtggcgtaag agagcgccgc cgcaccgctc gttggatgaa gattattagc
    20941 aaatgccagc gccggaggat cggtcatgga gccagccagc atcccgcaca tggtcaggta
    21001 attcattttg gctaacatcc gcgccagaat gccaacagta atcagcggaa cggcggtgat
    21061 cagggcacca taaccaatcc agcttagccc ttcgccatcc accagtgtat ggataaaatc
    21121 gccgccggat ttcaggccaa ccaccgagag aaagagcaca atgcctaact cgcgtagcgc
    21181 caggttagcg cttggtggca taaaccagta cagcttgccg atactgccga tacgcccgag
    21241 gatcaacgcc ataatcagcg gaccgcccgc cagcccgagt ttcaacgccg ccgggaaccc
    21301 aggcacaaag acgggaatag aacctaacaa tacgccaagc ccgataccaa taaacactgg
    21361 cagcatctga acctgttgca gtttttgctg cgcattcccc agcacattgg caacggcatc
    21421 aattgcggac ggacgcccca ccaggttgag gatatcgccg aactgcaggc tgatatcgct
    21481 acttgcaact aattcaacac cagcacggtt caggcgcgag ataacaacgt catagcgttc
    21541 tttaaagtgc aggtcgcgaa tacgtttacc gagcacgttt tcattggtga ccaccacacg
    21601 ctcgacgcgc aagtcagtgc ctttcgtaga cagcgaggta tcgacctcct gaccaatcac
    21661 cagttgcgca ttatgtaaat ccgcaggctg ccccaccaga tgcagcaaat cgcccagttg
    21721 aataacggtt tctggcgaag gcacttttag ggtttcttct cgtttcaatc gtgagcaaat
    21781 aactttatcg ccattgagaa tcggcacatc tttaatggct aaattatgca aattaggatt
    21841 ctcaactcgg atgttaatcg tcctgattag cgcaccgccg ttggttcgcg tggactcgtg
    21901 ctgctgggcc tcagtctcaa cattgacgcg gaaaataacc cgcagcatcc acatagtgaa
    21961 caaaatgccg caaatcccaa atggatacgc catcgcatag ctcatcccca tttgatcgac
    22021 catcgccatc ggtgtcccca ggtcgcgcaa gatctgctgc cctgccccca gtgctggcgt
    22081 attggttacc gcaccggaga aaatccccag tactaccggc agtggaatat caaacagttt
    22141 atgcaggatg gcggtaacca gccccccgat gatgacgatc agaacagcaa acaggttgag
    22201 gcgtaatccg gagacgcgca atgaggcaaa gaagcccggc cctacctgaa tcccgatggt
    22261 ataaacgaac aggatcaggc caaattcctg aataacatgc agcatatcgc tacttaatgt
    22321 catccccgcc tgagaaacaa aatggccgac gatgatccca ccgaacagca cgccgccaat
    22381 gcccaatcca acaccgcgaa acttgacgtt gccgataaac aacccgacga ccgccaccaa
    22441 agccagaata ctgaccgtta atgctatatc actcatcgtc catttctctt gagataacat
    22501 tttaattgct acggattatg gctgaatcgg tgaagcagat ttgccttata gcgcacatta
    22561 tcacgggaaa gaacgtgccg aaatatctta aacaggcgaa aattaacgca ttgttgaaga
    22621 agaaagacaa aaaaagcccc accagaatgg cggggcaaag agaatagcta gttagctatt
    22681 taacgcggga cgttcgctga tagcgatacg ctgcgctgcg atgggttcag gctcattacg
    22741 aattaaatca atatgcagta aaccgtttac gaaggttgca ccagagactt ccatattttc
    22801 agccagcgta aagctcaggc taaatggctg attcataagc ccttgatgca gccatttttt
    22861 ctcttctttt ggctgctccg gcgtgccttt tacgctcagg cgcgtacctt ccagttgaat
    22921 ctctaaatct tcctgacgaa aacctgccag cgcaagggta atgcggtagt ggttatcgtc
    22981 gcttttctca atgttgtacg gcgggaagct ctggctttca ccggcgtttt gcagtgcgtt
    23041 ggccagtttg tcaaaaccga tccattgacg catcagtggg gataaatcga agttacgcat
    23101 agtcatttct ccttctaaga agcgagtaag tacctgcaaa tccgaagatt cgcatatgct
    23161 ccctgacggc gagcatggag atgtcaggcc gcgcaaggcg gccttaggga attagttgat
    23221 ttcgatacgg cgcggttttt tcgcttccgg aatcacgcgt tcgagatcga tatacagcaa
    23281 accatttacc aggttagcac cacgaacatg aatgttctca gctaactgga atttgcgttc
    23341 aaagttgcgt tcagcgatgc cctggtacag ataggtgcgc tctttttgtt cgtcggcgtg
    23401 agcacctttc accaccagca gattatcctg ggcggtaatt tccagttcgc tctcagcaaa
    23461 accagccaca gcgatagcaa tgcggtaatg gttttcgtct accagttcaa cgttatacgg
    23521 agggtagccg ccattactct ggctctggtt gttttctaag tggttaaaca aacggtcaaa
    23581 tccaatagca gaacggtaaa gcggggataa atcaaagtta cgcataatca atagctcctg
    23641 aaatcagcga gaatctaaga ccttccacaa tggacaggtc aggtagccag aacacccatc
    23701 aggcgcgtcc tcatcggcta cgatgtaaaa atgggtctgg aaatgaactt ttcaagcctt
    23761 atcagcggac ttttttttgc agtttatggt ctattgcata gactgagggg gcagcacgaa
    23821 acgttaaagt gcgatagccg ccacagtgcg acgtaatgat gatgttattt tcagcaaggg
    23881 atcgctataa ctcatcaggc caacacccac gatcaacaga tgaatttatt atgataagaa
    23941 atgtgttgtt agcgttcatg atatgcagcg gaatgacatt acttggagga tgctccagcg
    24001 tgatgtccca taccgggggg aaagaaggaa cgtatcccgg cacccgcgcc agcgctacca
    24061 tgattggcga cgatgaaacc aactggggca ctaagtcgct ggcgattctc gatatgccgt
    24121 ttaccgctgt gatggacacg cttttactgc cgtgggacgt gttccgcaaa gacagctcgg
    24181 tgcgctcacg cgttgaaaaa agcgaagcta acgcgcaagc caccaatgcg gttattccac
    24241 ccgccagaat gcctgataac tgatttagcg atcagtttcg gtgatccaca gctggccgcc
    24301 ttccatccac gccagccact gaccatccgg cgagaagacc acggcgtccg cagaaggtgg
    24361 attggcgtgg ttttcggtta aatactcaac ctcgccgctt tgcgcatggg cgcaagcaat
    24421 tcgattatcc agcacaaagc ccaaccattc tcctgatgga tgccagttaa atgcagactg
    24481 aatatccgtt ttgttatggg ttaactggcg cggctcgccg ccctgtggcg agataagcca
    24541 cagttgcaca atgccgttat catcacgcat taaaaacgcg atttgcgtac cctgcggatt
    24601 acagcgcacc cagtggcgcg ggacgttgac taaccccgga taagcccgat ggtgggtaaa
    24661 ggttaaacgt cgctgcacga cgccacgcgg tggcgcgggc agggttgttt ccgttccact
    24721 taacggcgca tcaccagctg ctttccagcc agcttcatct tgcggtaact caacgataaa
    24781 tagctccggc actttctcgc cctttggcga aagtgtgtcg ccaataaacg ccagcgcgtg
    24841 atttcctacc catccttctt cataagcacg attgatttca ttactgccag gctgcggcgt
    24901 gggcgttgtt ttactcacca gcacgcacca gtggctaccg ctgtattcac gcggatgctg
    24961 cttttgtacg ttgaccgggc caaacggcgc agcaacacct acgtttcgca aatccagcgt
    25021 cgaatcgagt tgatgcatta catggtcgtt ataggtaaag ctcacccttt ctccgttcgg
    25081 gctaaagaca tgcacatggc tgccgccgcg cagcgctcct ggggtgtacg gcgcggtaat
    25141 atccattgca tcgagattgc tcaccttgcc gccttcagca atcactccgc gccgatgatg
    25201 gaagtcgtaa taccatgttt catcaggatt ttccgggccg tggatgaaaa catatttctc
    25261 tgacttcgga tgaacggtca ccacgccgac gtacgcgccc tgtgacgcgc gatagataac
    25321 ctcgacctcg ccggtatgga tattcacacg ctcaatggtc tcgccggtaa acgacgcccc
    25381 tgaaggacgc acgtcaaata ccagccactg gctatcgggc gtccaggtat tggtattggt
    25441 gagcaggtga ttacggggag caaaggttat ctgtttcatg gcgcggtcct gatacaaaac
    25501 gggtcccgcc atcatacttc acaaggactt cactttcaga caatagtctt ttgccatcgt
    25561 gatgaatgtg agtgaaaaga tggaacattt cgacgtggcg attattggcc tcggcccggc
    25621 agggtcggcg ttggcgcgaa agttagcagg caaaatgcag gtgatcgcgc tggataaaaa
    25681 gcaccagcat ggtactgaag gtttcagcaa accttgtggc ggtctgctgg caccggacgc
    25741 gcagcgatct tttattcgcg atggactgac gcttcctgtc gatgtcatcg ccaacccgca
    25801 gattttcagc gtcaaaaccg tcgacgtcgc cgcatcgctc acgcgtaact accagcgaag
    25861 ctatatcaat attaatcgcc atgctttcga cttgtggatg aaatcgctga tccccgccag
    25921 cgttgaggtt taccacgaca gcctgtgtcg aaaaatctgg cgtgaggatg ataaatggca
    25981 tgtcattttt cgcgcagacg gctgggagca gcatattacc gcccgctatc tggtcggtgc
    26041 cgatggtgcc aactcgatgg tgcggcgaca tctctacccg gatcatcaaa ttcgtaaata
    26101 tgtcgctatc cagcagtggt tcgcagagaa acatccggtg ccgttctact cctgcatctt
    26161 tgataatgcg ataactgact gttattcatg gagtatcagc aaagacggtt attttatctt
    26221 tggcggtgcc tatcccatga aagacggtca gacgcgtttc acgatgctga aagagaaaat
    26281 gagcgccttt cagttccagt ttggtaaggc ggtgaaaagc gaaaaatgca cggtgctgtt
    26341 tccctcacgc tggcaggatt ttgtctgcgg taaggacaat gcctttctga ttggtgaagc
    26401 ggcgggattt atcagcgcca gctcgctgga ggggattagc tatgcgctgg atagcgcaga
    26461 gattctgcgt tcggtgttac tgaagcagcc ggagaagagc aacgccgcct actggcgcgc
    26521 cacccgcaaa ctgcgtttaa aactcttcgg caagatagta aaaagccgat gcctgaccgc
    26581 accggcttta agaaagtgga ttatgcgcag tggtgtggcg catattccac agttgaaaga
    26641 ttagccaacg cgcttcacat cgcccaccag caggatgtaa gagagcgcgc caatcaacgc
    26701 gacggcggag atataaacca gtgcaggtgc gaaaccgtaa ccctgcgcca ggtaccccac
    26761 caccagcgga acggtgatgc cgcccagacc accggcgaag ttaaacacgc cgccggttaa
    26821 accaatcagg cgcatcggtg ccagagaaga gaccagcgac caggtaatcg aagcaaaacc
    26881 gttgccgaag aatgccagcg ccatcaggca cataatcatc atcggatcgt tggtgtagtt
    26941 agcgcccata atgcaggtgg agatcagcaa gccgcagata atcggcgttt tacgcgcaaa
    27001 gcccagtgaa aagcccttac gtaccagcag atccgcgacc cagccagaga gcaggacgcc
    27061 gacaaacgcc gcgaggaatg gcaccgtggt cataaagcct gctttcagcg ccgtgattcc
    27121 tttttcctgg gttaaatagt tcgggaacca agttaagaaa aaccacagtg tagaagccac
    27181 cgcaaattgc ccaagataga cgccgatcag tttacgatgg aacaccagtt tccagtcttt
    27241 ggctgttaac ggctgacgcg cctctttttt caccggcgca tcaccatcca ccagaccgcc
    27301 gccatcacga atgtaatcca gttcagcttt gctgatgcct ttagtcaggc gcggcggctg
    27361 ataaacctta aaccaaatca gcgaccagat aatgccgata ccaccggtga caatgaacac
    27421 ccagtgccag ctcaacattt cctgaatcca gatcagcagc ggagtcaaaa acgccagacc
    27481 gacaaactga ccagacgtat aaaaaccaac ggcggaagcg cgttcatgtt ccgggaacca
    27541 gctggtcacc atccggttat tggtcgggaa agcgggcgct tcgaaaatac cggttattgc
    27601 gcgcagacca attaatgaca tcaatcccgt ggcaaagccc tggaataaag tcgccactga
    27661 ccagccaaat atcgcaataa aataagtcac gcgtgaacct acgcgatcta aaaaccaacc
    27721 gccggggatc tgacatagtg tataaagcca ggcgaaggcc gaaaatacat agcccatttc
    27781 cgctttggta ataccgaact cttcctgaat atgggcggaa gccacggcca ggttagcgcg
    27841 gtcaacataa caaatgacca ccgtaataaa gatcatcacc agcgtcagat aacgccgacg
    27901 ccccggcttt gctgcattaa cgggaatatc catagcgagc tttctccaga ttttgggcat
    27961 agcgaagccg ctcaccatgc cctgtaattt acagagggtt atatttttgt attgctgttt
    28021 tagtgcccga tgagaggctt acgtggcagg aattaccact ctgctacgct gttatcttca
    28081 tgacgccaga gcggattacg ccagtccggg gcatttttac tgaactcaat aactttagct
    28141 tcgtcgattt ccacacctaa gcccggtttc gttaatggtt taaagaaacc gccgaccatg
    28201 ctgaagtctt ctttgttttt cacaaagtcg agtaactccg cgcctttgtt gtaatgaatg
    28261 cccatacttt gttcctgaag tacggcgtta taggaaacaa agtcgatatg caggcaagcc
    28321 gccagtgcaa tcggtccgag cggacagtgc ggcgcaaggg tcacgtcata ggcttctgcc
    28381 attccggcaa ttttgtagca ttcggtaata ccgcccgcgt gggagagatc tggttgcaga
    28441 atcgaaatac cacccgcctc cagcacacgt ttaaaatcga aacgtgagaa catgcgttca
    28501 cccgccgcca gtggaatatg cgtttgtgcc gccagtttcg ggtagtattc ggcttgttct
    28561 gccagcaccg gctcttcaat aaacagcggg cgatacggct ccagttcttt aatcagcact
    28621 ttcgccatcg gcgcgctgac gcgaccgtgg aaatcaagac caaactcaat ctgattgcca
    28681 aaagcttcac gaatttgtgc cacggtgttg actgccgcat ctaccgcgcg ggagttatca
    28741 attagcccca gttcttcaca accgttcagt ttgaaggtat cgaagccgat ttcgcgcagg
    28801 gttttaatgc cgtcgataac atccgccgga cgatcgccgc cgacccaact gtaggcttta
    28861 attttgtcgc gaaccaggcc gcccatcagt tgccagaccg gcgcattcag cactttgcct
    28921 ttgatatccc ataacgcctg gtcgatcccg gcgatggcgc tcatcaggat tggaccgcca
    28981 cgataaaatc cggcgcgata catcacttgc cataagtcat tgatgcgcga tggatcctga
    29041 ccaatcaaat agtcacccag ctcgtgaact gccgcttcca ccgtgcgggc gcggccttca
    29101 atcactggct cgccccagcc gaccacgcct tcatcggttt caattttcag gaacatccag
    29161 cggggaggta aacgatacgt ggtaattttg gtgattttca ttgcactgcc tctcgatacg
    29221 ccttaacaaa tgctgctgcc tgctgcgcgg tgcgttctac ggactgcccg gcgcgataga
    29281 gatcgctgcc taagcccgcc cctgcacaac ctgcgtctat ccactgcgcc aggttttctg
    29341 gcgtcacgcc gccaacggca aagactgcga tgtccggtgg taataccgct tttaacgctt
    29401 tgatgtattg cggaccaaaa gccgatgacg gaaatatttt cagcgcctga gcgcccgcgt
    29461 cgagcgcggt aaaggcttcg gtcgccgtcg cgcagccggg gcaaacggtc atgccgtagc
    29521 ccaccgcacg gcggatcact tcactatgga tattgggcgt aacgatgagc tggcagctca
    29581 tcctggcgag tgcatcgacc tgttcaggtt tcagtaccgt acctgcgcca atcaacgcct
    29641 tatcgccata cgcatcaacg atggcgggaa tgcttttctc ccattgaggg gaattcagcg
    29701 ggatttcaac cgcgtcgaac ccggcgtcaa tcaccgcgcc aacatgcgcc agcgcctcgt
    29761 cgggcgtaat gccgcgcaaa atggcgatca gcgggagttt agtttgccac tgcatgagcg
    29821 atgctcctta taccagcctg aaatgccgtg tcgcccgcca ccgccgtcac gtcgcaaccc
    29881 atcgcctgaa aggcttgctg gtagcgcgcg gtcagcgatg ttccggcgac aagggtgatg
    29941 gcgtgttgat gggtcacata gtcgcgcata ctggcaacct ctgcgccaat caacaaacca
    30001 gagagaaatt cgctgacctg ttcgcgggga agtgttccca gcacatgcga ggcgcgaact
    30061 tcaaaaagct gcggcaatat ggcgggagca ttaaagccac gctcaagacc agccgcgaag
    30121 gcatcggcag agttttcctg cggcggcaaa cctgcgccaa tcaatgagtg atttaacagt
    30181 aaatgatgta attcaccggt catcacggtg cgaaaatcgt tgatttgctg gctatcggcc
    30241 tgcacccatt tgcaatgggt accgggcata acataaagag aggaaggagc gagagcacgc
    30301 gcgccgatca attgtgtttc ttcgccgcgc atcacattgt ggttatcgtc atgagagacg
    30361 cataatccgg gaataatcca gatattgtcg ccaaccgacg ttaattgatc gccaatagac
    30421 gaaaaacggg caggaacaga taaatacggt gcaactttcc agccgacatt gctgccgacc
    30481 attcctgcca tcaccactgg cgtattctct tcacgccagt cggtcgtgac ttctgctaac
    30541 accgcagccg gagattttcc gttcaggcgc gtgacgcctg cttctgattg cctgctgtcc
    30601 aggcagtggt cgccctgata aagccaggcg cgcagattgg tcgatcccca gtcaattgcg
    30661 atgtagcgag ctgtcatgtg atttccttta accttcgtgt cgagctggcg atcatggtaa
    30721 gcgccgcctg ctctgccgca tcgccgtcct gatgccgtat tgcatcgaac agcgccttat
    30781 gttcctggag cgtttgcggc atgttggcct catcgcccat ccaggttcgt tcaaaaaccg
    30841 cccgctgcag cgaactgatc gcaatgctaa gttgctgtaa caccgggtta tgcaccgact
    30901 gcagcaccgc ctcgtggtag cgaatatccg cttcgttaaa cgcttcgcgg tcctgattgt
    30961 tggcaatcat ctcgttcagc gccgattcaa tctgcgccag atcgctggag gtcgcgcgct
    31021 ctgctgccca acgggcaata gccggctcta ccaggtttcg cacttcgctc attgcactga
    31081 taagccgtgg gtcatagtcg ttttccagca cccattgcag tacgtcagtg tcgaggtaat
    31141 tccactggtt acgcggagcc acaaacgccc cgcgataacg tttcatttca atcagccgct
    31201 tcgccatcag cgaacgaaac acctcacgga tgatgttgcg cgaggttgca aactcttcac
    31261 agagctccgc ctcagccgga agtggagagc ctggtacgta tttgccgtga acgatctgtt
    31321 tacccagcgt aataacgatg cgatcggttt tattgagagt catggagagt ccttgtgctc
    31381 tgtatgttct tctctacttt accccgatcg atgcataacg cggcaacttt gtagtaccag
    31441 cgtgatgacg ttcgcgtttg ctgtgcgcct aatgtagtac aaacttatat tgttgtacta
    31501 caatttagat cacaaaaaga acaatacata aaaaatgaca tgcgtcgggc agaaatctga
    31561 aaagggatgt caggcgctaa acaggaggga aagaagagta tgctttcaac ggcttagcta
    31621 ctcgtttaaa ggattaatca tgaagttgaa ttttaaggga ttttttaagg ctgccggttt
    31681 gtgttctttg gcatttgcgt tgaccggatg tatcacctgg ggattggttt ccaatactgc
    31741 aagccgtcca catgccgagc aatggcggtc agacacgatt aagggtttat cgctggctga
    31801 agacagtaac ggtacgaaag gctatgtttt tgttggtgaa tcgttggact atctgttgac
    31861 caccggcggc gatgaggtgg ttaaaatgct gaacgatccg gcaatccatg gtgaaagaat
    31921 caccgtttct gataacgcga aatttatttt aagcagcagt aataagaatt tctctggtgc
    31981 tattacgctc tattacgact ggaacaacga agaagataag gcactggcaa cacaatatgg
    32041 ttttatttgt gatacccggc gttgtacatg gatgctggac ggtttgaaag gctcaatcca
    32101 ccagaaaaat aaaaaagcgg attactcaaa cgtgatggtt ttccatcaac cctttacggt
    32161 gggattttat gagtacaaag caacagatgg cgtcccgcgc ggcctcgtta atgcgctgct
    32221 gccagtcact cttacgctgg atatagtaac gtcaccactc cagttcctga ttttatgcac
    32281 gaccagaaat tgttaatcgc tgcgggcaaa cgcattcatg ccgtttgccc attcactaat
    32341 taattcagca catacttctc aatagcaaat gccacgccat cttcaaggtt ggatttggtg
    32401 acaaagttcg ccacttcttt caccgaagga atagcgttat ccatcgccac accgacgcct
    32461 gcatattcaa tcattgcgat gtcgttttcc tgatcgccaa tcgccatgat ttcttccggt
    32521 ttaataccta acacgtcggc cagtgatttc acacccgtac ctttgttaac gcgtttatcg
    32581 aggatttcga ggaagtacgg cgcacttttc agcacggtgt atttttcttt cacttcctgc
    32641 ggaatacgcg cgatggcctg gtcgaggatg gcaggttcat caatcatcat cactttcagg
    32701 aactgggtat tggggtccat tttctccgct tcgcagaaca ccagcggaat ggtggcaacg
    32761 aaagattcat gcaccgtgta gtagctgata tcacgattgg cggtgtacag cgtggtgcgg
    32821 tccagggcgt ggaaatgaga accgacttcg cgagagagtt tttccaggaa acggtagtcg
    32881 tcatagctga gagtggtttg cgccacggtg ctaccatcag cggccttctg taccagcgcg
    32941 ccgttataag taatgcagta gtcgcccggc tgttccatat gcagctcttt caggtaattg
    33001 tgcacacctg cataagggcg acccgtcgtt agcacgacat tcacgccacg ggcgcgagct
    33061 gcggcaatcg catttttaac ggcgggtgaa atggtgtgat cgggcagcag aagggtgcca
    33121 tccatatcga tagcaatgag tttgatagcc atgagttccc caggtagatt ggttcctgac
    33181 ccatgctaac gcgattccgc tcaaaaatca gtacaacacc cgagggaaaa gggggatgca
    33241 acgcgcgttg cgtgctcccc ttttgcttag cggaagagtt tccctttcag cagttccatg
    33301 cctgcggaaa gcagatcgtt attggcttgt ggtgacactt caccttgcgg tgagagtgca
    33361 tcaataatct tcggcaattg ttctgccagt aaactggaag ctgtactggt atccacgccc
    33421 agtttttgcc cgagatcgga caccgcattt gtgccgagcg ccgattccag ttgctcgccg
    33481 ctaactggtt ggttgccctg ttgattactc agccaggttg agagaatggc acctaaaccg
    33541 ccactttgca gtttttccag cagcacctga atgccgccct gctcctcaac ccaacttaaa
    33601 atcgcctgat atttccccgc atcgcctttc agaaaggcgc cgacaacgtc atcaaaaagg
    33661 cccatgataa tcacctgtaa agcgttacgt gttgactcaa aaagtatagt tctgtgggat
    33721 gataattgcg gattgcagga ataaaaaggg cggagatgat ctccgccctt ttcttatagc
    33781 ttcttgccgg atgcggcgtg aacgccttat ccggcctaca aaaacatgca aattcaatat
    33841 attgcaggag tctcgtaggc ctgataagcg tagcgcatca ggcacgctcg cattgttaac
    33901 gccattaaat atcaatattc gccgctttca gagcgttctc ttcaataaac gcacggcgcg
    33961 gttcaacggc gtcgcccata agcgtagtga acaactggtc ggcagcaatc gcatctttaa
    34021 cggtaacgcg cagcatacga cggctttccg ggtccatggt ggtttcccac agctgttccg
    34081 ggttcatctc gcccagacct ttataacgct ggatggagag gccgcgacgg gactctttca
    34141 ccagccagtc cagcgcctgc tcgaagctgg ctaccggctg acgacgttcg ccacgttcga
    34201 taaatgcatc ttcttccagc aagccacgca gtttctcacc cagcgtgcag atacgacgat
    34261 attcgccgcc agtgataaat tcgtgatcca gcggatagtc agtatccaca ccgtgggtac
    34321 gcacgcgaac aatcggctcg aacaggtttt gttcggcatt ggtgtggaca tcaaacttcc
    34381 actggctgcc gtgctgttct ttgtcgttca gttcgctgac cagcgcgttc acccagcggg
    34441 taacggtctg ctcatcagag aggtcggctt ccgtcagcgt cggctgatag ataagctctt
    34501 tcagcattgc tttcggataa cgacgctcca tgcggttgat cattttctgc gtcgcgttgt
    34561 actcagacac cagtttctct aacgcttcgc cagccaatgc cggtgcactg gcgttggtgt
    34621 gcagcgttgc gccatccagc gcgatggaga tctggtactg atccatcgct tcgtcgtctt
    34681 taatgtactg ttcctgcttg cctttcttca ctttgtacag cggcggctga gcgatgtaga
    34741 cgtgaccgcg ttcaacgatt tccggcatct gacgatagaa gaaggtcaac agcagcgtac
    34801 gaatgtgcga gccgtcgacg tccgcatcgg tcatgatgat gatgctgtga taacgcagtt
    34861 tgtccgggtt gtactcgtca cgaccgatac cacaaccaag cgcggtgata agcgtcgcca
    34921 cttcctggga agagagcatc ttatcgaagc gcgctttctc gacgttgagg attttaccct
    34981 tcagcggcag aatcgcctgg ttcttgcggt tacgcccctg cttcgcagag ccgcccgcgg
    35041 agtccccttc caccaggtac agttcagaga gcgccggatc gcgttcctgg cagtctgcca
    35101 gtttgcccgg caggcccgct aagtcgagcg cacctttacg gcgggtcatt tcacgcgcgc
    35161 gacgcgccgc ttcacgggca cgggcagcat cgataatttt gccaaccacg attttcgcgt
    35221 cggttgggtt ttccagcagg tattctgcca gcagttcgtt catctgctgc tcaaccgccg
    35281 atttcacctc agaagaaacc agtttgtctt tggtctggga ggagaatttc gggtccggca
    35341 ctttcacgga aacgaccgca atcaggcctt cacgcgcatc gtcaccggtg gcgctgactt
    35401 tggctttttt gctgtagcct tctttgtcca tgtaggcgtt cagggtacgg gtcatcgccg
    35461 cacggaagcc tgccaggtga gtaccgccgt cacgctgcgg aatgttgttg gtaaagcagt
    35521 agatgttttc ctggaagcca tcgttccact gcaacgccac ttcgacgcca ataccgtctt
    35581 tctcggtgga gaagtagaag atattcgggt ggatcggcgt tttgttcttg ttcagatatt
    35641 caacgaacgc cttgatgccg ccttcatagt ggaagtggtc ttctttgccg tcgcgcttgt
    35701 cgcgcagacg aatggaaacg ccggagttga ggaacgacaa ctcacgcaga cgtttcgcca
    35761 gaatttcata ttcgaactcg gtcacattgg tgaaggtttc gaggcttggc cagaaacgca
    35821 ccatggtgcc ggttttttca gtctcgccgg taaccgccag cggggcctgc ggtacaccgt
    35881 gttcgtagat ctgacggtga attttaccct cgcgctggat aaccagctcc agtttttgcg
    35941 acagggcgtt tactaccgaa acaccaacgc cgtgcagacc gccggacact ttataggagt
    36001 tatcgtcaaa tttaccgcct gcgtgcagaa cggtcatgat cacttccgcc gccgatacgc
    36061 cctcttccgg gtgaataccg gtcggaatgc cgcgcccgtc atcctgtaca gagacagagt
    36121 tatcggcgtg aatggtgacg ataatttctt tacagtgacc cgcgagcgct tcgtcgatag
    36181 cgttatctac cacctcgaat accatgtggt gcagaccggt gccgtcatcc gtgtcgccga
    36241 tatacatacc cgggcgctta cgcaccgcat ccagcccttt caggactttg atactggagg
    36301 agtcataaga attcgacatc aacgtttctc gctcatttat acttgggtta atccgttatt
    36361 ttaccctttt ccacggtaaa catcttcgaa ttttcgtccg acatgtctat aacgtgttca
    36421 gcactgatcg cgctgacaaa gacctgtgat tgcgtcgctt ttaagcggct ggcaagcagc
    36481 ccgcgacgct catcatcaag ctcagaggca aaatcatcta tcaggtagag acaccgccgc
    36541 ccgctttcac gggtgaggaa ctctccttgc gccagacgta aggcgcacat caacagctta
    36601 agctgcccac gcgataaggt atcttccacc ggcgcaccgt cggcgcgaat gcgtaagtcc
    36661 gctttatgcg ggccgtgcgc ggtgtaggtt agctggcgat cgcgttcaaa attacgttcc
    36721 agcacctcag cgtactctgt ctctttctcc cagccgcgct ggaaagagaa agtcagagaa
    36781 aactcaggga gaaattgctt acaggtatcg gccatatcag ccgcgatacc ggcgctatac
    36841 tccgcgcgcc aggtgcttat ttgctccgcc agcggaatca gttctttatc ccacgggcgt
    36901 agctgttcgt aacgtgtcac ctggcgcagc gccgcattgc gctgcttgag caatcgcttg
    36961 agattgctcc aggcggtgaa aaatccgggt tcgttgtgaa agcatcccca gtcgaggaat
    37021 gctcttctgt atttggggcc gccgttgagt aaagtaaacc cttctggcgt tatcaactgc
    37081 attggcatca ggtgcgccag ttccgcgacc ttatgcccgt ctgtaccgtc gatgcggact
    37141 ttgctgtcgc cctgtttgtc tttggttaag ccaatcgccg tctcgcgctc ttcgccctgt
    37201 aatcgcccgt ggagaacaaa cgcctcctgc tcgtggcgaa tgacgcgacc aatctgcaaa
    37261 ctgcgaaacg cccgaccatg gccgagcgta tagatggctt ccagcacgct ggttttgcca
    37321 ctaccgttgg cacctaccag aaagttaaag ccgggagata aagcgagatc cgcggtttcg
    37381 atattgcgga aatcgcggat caacaagcgg gtgagggaca ttacagtctc attggcatga
    37441 caacataggc agccgactgt gatgcggcat cttcaatctg cacgctggaa accgaatcgg
    37501 tcagcatcat gcggacgttt tcgcatttca gtgcgttcag aacatccagc acatagctga
    37561 cgttgaagcc gatttccatc tccgcaccgc tataggtaac gtcgaggatc tcttccgctt
    37621 cttcctgttc tgggttgttg gcggtgattt tcagctggtt ttcgctgaca tacagacgca
    37681 cgccgcggaa tttctcgtta gagagaattg ccgcacgggc aaacgcctgc ttgagcagat
    37741 cacagccagc ttccagatgc ttgtccgggt tcttcggcag aacgcgacgg taatccggga
    37801 agcgaccatc caccagtttc gaggtgaaga taaagtcgcc aacgtgggcg cgaatgttgt
    37861 tgctgccaat ctgcacgcgc agcggattgt cgccgccgtc gagcatacgc atcagttcaa
    37921 tcacgccttt acgcggcacg atcaccgaat ggcttggcaa agattgacca attggcattg
    37981 aacagactgc cagacggtgg ccgtcggtcg ccacggtgcg cagttcttca ccttcggttt
    38041 caaacagcat accgtttaag taatagcgaa cgtcctgatg cgccatagaa aactgggtcg
    38101 cttcaatcag acgcttcatc gtcgcctgcg gcagggtaaa ttcgacttcg ctctgccagt
    38161 catcgaggtt cgggaaatct gccgctggca gggtagacag cgaaaaacgg ctacgcccgg
    38221 agcgcaccag catccgctca ccttccagct gcacggcaat ttccgcgcct tcaggcagac
    38281 cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt cgcccctggt tcgtgcggct
    38341 gaaccagcgc aacgcgtgcc accatttcca tctcgagatc agtaccggtc agcgacaacg
    38401 taccgtcagc aacctgtaac agcagattac cgagaatcgg tagcgtagga cgaccaccta
    38461 acggaccgct cacctgttgt agcggtttta ataaatgctc acgttctacg gtaaatttca
    38521 taggtttacg atgacaatgt tctgattaaa ttagaaaaat cttctttgat atcgtggctc
    38581 tcttcacgca actgctcgat cttccggcag gcatgaagca ccgtcgtgtg gtcacgacca
    38641 ccaaaggcat cgccaatctc cggaagactg tggttagtca gctctttcgc cagcgccatc
    38701 gccatttggc gcggacgggc caccgagcgg gatcgacgct tggaaaggag atccgcgact
    38761 ttgatcttgt agtactccgc caccgtcttc tgaatattgt cgatggtgac cagtttttcc
    38821 tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt cgatggtgat cgcccgtccg
    38881 gtaaagttgg cattggcaat gacgcggttc agcgcgcctt ccaactcacg tacgttagat
    38941 cgtagacgct tggcgataaa aaacgccact tcgcccggca aacgaatgtc gttttcgtcg
    39001 gcctttttca tcaggatcgc cacgcgggtt tccagctctg gcggttcgat cgccacagtc
    39061 agtccccagc cgaagcggga tttcaaacga tcctcaacgc cgttgatctc tttcggatag
    39121 cgatcggagg tgagaatgat ctgttgatta ccttccagca gggcgttgaa ggtgtggaaa
    39181 aactcttcct gagatcgttc tttattagca aaaaactgaa tatcgtcgat cagcagtgca
    39241 tctacggaac ggtagtagcg tttaaactct tcgatcgcgt tgttttgcag ggctttaacc
    39301 atgtcctgaa caaagcgctc ggagtgcata taaaccactt tggcattcgg cttgcgcgcc
    39361 ataatgccgt tacccaccgc atgcagcagg tgagttttac ccaaacccgt gccgccataa
    39421 aggaacaacg ggttataagc accgccaggg ttatctgcca cctggcgagc cgccgcgcgc
    39481 gccagttggt tagatttacc ttcaacgaag ttatcaaacg tgtgtttgac gtttacgtta
    39541 gaacgatagg tcggttctgc cggagccggg acgttatccc aacccgagcg cgtagaaggc
    39601 gcagcacgtt gcggctgcgt ttgcgccacc tgtgcagggg ccgcgacgtt gctcgtcact
    39661 gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt caaaacgcag ctgtggggca
    39721 tccgctccgc agaaactggt tagcagtcca ttgatattat taaggtactt gtcccgtacc
    39781 caatcgagga caaaacggtt tggcgcgtac agggccagcg tgttatcgct cagttccgcc
    39841 tgcaatgggc gtatccacat actgaattct gtggctggta actcatcctg caatcgggca
    39901 agacactgct gccaaagcga aagtgacacg gcggactcca ctcgaacaaa agtcgataat
    39961 gactaaggct gaaacattca tgattgttga cgtacgtcga aaagaccctg catgagggtg
    40021 acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga tcctgcaaaa cgatcgggac
    40081 cgcggatcat agcctaaact gcgcaagaga tcttctgttt ctcacagatt ttcccgattt
    40141 atccacagga ctttccagaa ctcgctaagt gtaaacgatc ctgccgcggg ccgggcacga
    40201 tttacgccgc atattggaaa atttaatgac catagacaaa aattggctta atcgatctaa
    40261 taaagatcca ggacgatcct tgcgctttac ccatcagccc gtataatcct ccacccggcg
    40321 cgccatgctg gttttcactg gtgtgaggcc gtacattttc cctgcgaaaa ggtgcggaaa
    40381 agcgcggtaa ataaggaaag agaattgact ccggagtgta caattattac aatccggcct
    40441 ctttaatcac ccatggcttc ggtgtccatc gtttcatttt tcggcggata tccaataaag
    40501 ccattgaatt tattcaagtt taggtagaaa tcgccatgaa acgcactttt caaccgtctg
    40561 tactgaagcg caaccgttct cacggcttcc gtgctcgtat ggctactaaa aatggtcgtc
    40621 aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct gaccgtttct aagtaataaa
    40681 gctaacccct gagtggttaa gctcgcattt cccagggagt tacgcttgtt aactcccagt
    40741 caattcacat tcgtcttcca gcagccacaa cgggctggca cgccgcaaat taccattctc
    40801 ggccgcctga attcgctggg gcatccccgt atcggtctta cagtcgccaa gaaaaacgtt
    40861 cgacgcgccc atgaacgcaa tcggattaaa cgtctgacgc gtgaaagctt ccgtctgcgc
    40921 caacatgaac tcccggctat ggatttcgtg gtggtggcga aaaaaggggt tgccgacctc
    40981 gataaccgtg ctctctcgga agcgttggaa aaattatggc gccgccactg tcgcctggct
    41041 cgcgggtcct gatagccctc attcgggtct atcaacgcct gattagtccg ctactcgggc
    41101 cgcattgtcg tttcactcca acctgttcaa gctacggaat tgaggcattg cgcaggtttg
    41161 gagtgataaa aggcagttgg ttgacggtga aacgcgtatt aaaatgccac cctttacacc
    41221 ctggtggtga cgatcccgtc ccgcccggac catttgatac cagagaacac taacgatgga
    41281 ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg tctttcatga tctggcaagc
    41341 ctgggagcag gataaaaacc cgcaacctca ggcccaacag accacgcaga caacgaccac
    41401 cgcagcgggt agcgccgccg accagggcgt accggccagt ggccagggga aactgatctc
    41461 ggttaagacc gacgtgcttg atctgaccat caacacccgt ggtggtgatg ttgagcaagc
    41521 tctgctgcct gcttacccga aagagctgaa ctctacccag ccgttccagc tgctggaaac
    41581 ttcaccgcag tttatttatc aggcacagag cggtctgacc ggtcgtgatg gcccggataa
    41641 cccggctaac ggcccgcgtc cgctgtataa cgttgaaaag gacgcttatg tgctggctga
    41701 aggtcaaaac gaactgcagg tgccgatgac gtataccgac gcggcaggca acacgtttac
    41761 caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac gtcaactaca acgtgcagaa
    41821 cgctggcgag aaaccgctgg aaatctcctc gtttggtcag ttgaagcaat ccatcactct
    41881 gccaccgcat ctcgataccg gaagcagcaa cttcgcactg cacaccttcc gtggcgcggc
    41941 gtactccacg cctgacgaga agtacgagaa atacaagttc gataccattg ccgataacga
    42001 aaacctgaac atctcttcga aaggtggttg ggtggcgatg ctgcaacagt atttcgcgac
    42061 ggcatggatc ccgcataacg acggtaccaa caacttctat accgctaatc tgggtaacgg
    42121 catcgccgct atcggctata aatctcagcc ggtactggtt cagcctggtc agactggcgc
    42181 gatgaacagc accctgtggg ttggcccgga aatccaggac aaaatggcag ctgttgctcc
    42241 gcacctggat ctgaccgttg attacggttg gttgtggttc atttctcagc cgctgttcaa
    42301 actgctgaaa tggatccata gctttgtggg taactggggc ttctccatta tcatcatcac
    42361 ctttatcgtt cgtggcatca tgtacccgct gaccaaagcg cagtacacct ccatggcgaa
    42421 gatgcgtatg ctgcagccga agattcaggc aatgcgtgag cgtctgggcg atgacaaaca
    42481 gcgtatcagc caggaaatga tggcgctgta caaagctgag aaggttaacc cgctgggcgg
    42541 ctgcttcccg ctgctgatcc agatgccaat cttcctggcg ttgtactaca tgctgatggg
    42601 ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc cacgacctgt cggcacagga
    42661 cccgtactac atcctgccga tcctgatggg cgtaacgatg ttcttcattc agaagatgtc
    42721 gccgaccact gtgaccgacc cgatgcagca gaagatcatg acctttatgc cggtcatctt
    42781 caccgtgttc ttcctgtggt tcccgtcagg tctggtgctg tactatatcg tcagcaacct
    42841 ggtaaccatt atccagcagc agctgattta ccgtggtctg gaaaaacgtg gcctgcatag
    42901 ccgcgagaag aaaaaatcct gattcggtga gttttcgcta aaataagggc ggtcagttga
    42961 ccgccttttt tcttttcgta gggcggataa gcaccgcaca tccgccacac aaagcaacag
    43021 gaacatcatg agcgataatg acactatcgt agcccaggcc acgcctccgg gacgtggcgg
    43081 cgttggcatc ctgcgcatct ccggcttcaa agcccgtgaa gttgccgaaa ccgtgctggg
    43141 taaactgcct aagccgcgct acgccgatta tcttccgttt aaagacgccg acggcagcgt
    43201 gctcgatcag gggattgcgc tatggttccc tggcccgaac tcgttcaccg gcgaagatgt
    43261 gctggaactg caaggtcatg gcggtccggt gatcctcgac ctgctgttaa aacgcattct
    43321 gaccattccc ggcctgcgga ttgctcgccc gggtgagttt tccgaacgcg cgtttcttaa
    43381 cgataaactc gacttagccc aggccgaggc gattgcggat cttatcgacg ccagttcgga
    43441 acaggcggcc cgttcggcac ttaactcgct gcaaggcgca ttctccgcac gggttaatca
    43501 tctggtagaa gccctcaccc acttgcgcat ttacgttgaa gcggcaattg atttccccga
    43561 tgaagagatc gatttcctct ccgacggcaa aattgaagcc cagcttaatg acgtgattgc
    43621 cgatctcgat gcagtgcgtg ctgaagcacg tcagggtagt ttgttgcgcg aagggatgaa
    43681 agtggtgatt gccggacgtc ctaacgccgg taaatcgagc ctgttaaacg cgctggctgg
    43741 ccgtgaagca gcaatcgtaa ccgatatcgc cggaactacg cgtgacgtgt tgcgtgagca
    43801 tatccacatt gacggaatgc cgctgcatat catcgacacc gccgggctac gtgaagccag
    43861 cgatgaagtg gaacgtattg gtatcgagcg cgcgtggcaa gaaattgaac aagccgaccg
    43921 cgtgctgttt atggtcgatg gcaccacgac agacgccgtt gatccagcag agatctggcc
    43981 ggagtttatc gcccgtctgc cagcgaaact gccgatcacc gtggtgcgca ataaagccga
    44041 tatcaccgga gaaacgttgg ggatgagcga agtgaacggt cacgcgttaa ttcgtctctc
    44101 tgcaaggact ggtgaaggtg tggacgtgct gcgtaaccat ctcaaacaga gcatgggctt
    44161 tgacaccaac atggaaggcg gcttcctggc gcgtcgtcgt cacctacagg cactggaaca
    44221 ggcggcagag cacctgcaac agggtaaagc gcaactgtta ggagcctggg caggcgagct
    44281 gctggcggaa gaactgcgtc tggcgcagca gaacttaagc gagatcaccg gggaatttac
    44341 ttctgacgac ctgctggggc gaattttctc cagcttctgt attggtaag
//
LOCUS       DAZKGF010000019        43409 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZKGF010000019 DAZKGF010000000
VERSION     DAZKGF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.019"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            156..1739
                     /gene="yegH"
                     /locus_tag="ACHOQV_001019"
     CDS             156..1739
                     /gene="yegH"
                     /locus_tag="ACHOQV_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP9629432.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
     gene            1814..2152
                     /locus_tag="ACHOQV_001020"
     CDS             1814..2152
                     /locus_tag="ACHOQV_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9629433.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSNIDLAKRRYK
                     ELVQERKNEN"
     gene            2142..2432
                     /locus_tag="ACHOQV_001021"
     CDS             2142..2432
                     /locus_tag="ACHOQV_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9629434.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(2485..4338)
                     /gene="asmA"
                     /locus_tag="ACHOQV_001022"
     CDS             complement(2485..4338)
                     /gene="asmA"
                     /locus_tag="ACHOQV_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFP9629435.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQVNWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLKETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVIGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4360..4941)
                     /gene="dcd"
                     /locus_tag="ACHOQV_001023"
     CDS             complement(4360..4941)
                     /gene="dcd"
                     /locus_tag="ACHOQV_001023"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFP9629436.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5033..5674)
                     /gene="udk"
                     /locus_tag="ACHOQV_001024"
     CDS             complement(5033..5674)
                     /gene="udk"
                     /locus_tag="ACHOQV_001024"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP9629437.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5992..9309
                     /gene="dgcE"
                     /locus_tag="ACHOQV_001025"
     CDS             5992..9309
                     /gene="dgcE"
                     /locus_tag="ACHOQV_001025"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9629438.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNMADWLRLALGSAIVPPLLGGVLVILLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9418..10266)
                     /gene="alkA"
                     /locus_tag="ACHOQV_001026"
     CDS             complement(9418..10266)
                     /gene="alkA"
                     /locus_tag="ACHOQV_001026"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFP9629439.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADDYYARSLAVGEC
                     RGVVTAIPDIASHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPLRSYALLHIWYTEG
                     WQPDEA"
     gene            10400..11752
                     /gene="yegD"
                     /locus_tag="ACHOQV_001027"
     CDS             10400..11752
                     /gene="yegD"
                     /locus_tag="ACHOQV_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9629440.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11765..13704)
                     /gene="yegI"
                     /locus_tag="ACHOQV_001028"
                     /pseudo
     CDS             complement(11765..13704)
                     /gene="yegI"
                     /locus_tag="ACHOQV_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(13701..14462)
                     /gene="pphC"
                     /locus_tag="ACHOQV_001029"
     CDS             complement(13701..14462)
                     /gene="pphC"
                     /locus_tag="ACHOQV_001029"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFP9629441.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSASQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYTNMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSGPAVNERTDDDKTLALALWLP"
     gene            complement(14459..15118)
                     /gene="yegL"
                     /locus_tag="ACHOQV_001030"
     CDS             complement(14459..15118)
                     /gene="yegL"
                     /locus_tag="ACHOQV_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP9629442.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15339..15398)
                     /gene="ibsA"
                     /locus_tag="ACHOQV_001031"
     CDS             complement(15339..15398)
                     /gene="ibsA"
                     /locus_tag="ACHOQV_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFP9629443.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15671..15727)
                     /locus_tag="ACHOQV_001032"
     CDS             complement(15671..15727)
                     /locus_tag="ACHOQV_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9629444.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            complement(15999..16055)
                     /locus_tag="ACHOQV_001033"
     CDS             complement(15999..16055)
                     /locus_tag="ACHOQV_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9629445.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(16327..16383)
                     /locus_tag="ACHOQV_001034"
     CDS             complement(16327..16383)
                     /locus_tag="ACHOQV_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9629446.1"
                     /translation="MMKILIIVVLLVVSYPAY"
     gene            16662..17909
                     /gene="mdtA"
                     /locus_tag="ACHOQV_001035"
     CDS             16662..17909
                     /gene="mdtA"
                     /locus_tag="ACHOQV_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFP9629447.1"
                     /translation="MKSSYKSRWVMVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17909..21031
                     /gene="mdtB"
                     /locus_tag="ACHOQV_001036"
     CDS             17909..21031
                     /gene="mdtB"
                     /locus_tag="ACHOQV_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFP9629448.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGQGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            21032..24109
                     /gene="mdtC"
                     /locus_tag="ACHOQV_001037"
     CDS             21032..24109
                     /gene="mdtC"
                     /locus_tag="ACHOQV_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFP9629449.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRIGLNPQALFNQGVSLDDVRSAISNANVRKPQG
                     ALEDDTHRWQVQTNDELKTAAEYQPLIIHYNNGGALRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            24110..25525
                     /gene="mdtD"
                     /locus_tag="ACHOQV_001038"
     CDS             24110..25525
                     /gene="mdtD"
                     /locus_tag="ACHOQV_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9629450.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQIGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAITTLMLMPNYTMQTRCFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNCALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFVF
                     ARVPSDTHQNVAISRRKRSAQ"
     gene            25522..26925
                     /gene="baeS"
                     /locus_tag="ACHOQV_001039"
     CDS             25522..26925
                     /gene="baeS"
                     /locus_tag="ACHOQV_001039"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFP9629451.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26922..27644
                     /gene="baeR"
                     /locus_tag="ACHOQV_001040"
     CDS             26922..27644
                     /gene="baeR"
                     /locus_tag="ACHOQV_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFP9629452.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLMIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLEALDEE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27835..28167
                     /gene="yegP"
                     /locus_tag="ACHOQV_001041"
     CDS             27835..28167
                     /gene="yegP"
                     /locus_tag="ACHOQV_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFP9629453.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            28315..29676
                     /gene="yegQ"
                     /locus_tag="ACHOQV_001042"
     CDS             28315..29676
                     /gene="yegQ"
                     /locus_tag="ACHOQV_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFP9629454.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTTFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGELAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29949..30167)
                     /gene="ogrK"
                     /locus_tag="ACHOQV_001043"
     CDS             complement(29949..30167)
                     /gene="ogrK"
                     /locus_tag="ACHOQV_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFP9629455.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(30249..31412)
                     /locus_tag="ACHOQV_001044"
     CDS             complement(30249..31412)
                     /locus_tag="ACHOQV_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFP9629456.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDISGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(31412..31891)
                     /locus_tag="ACHOQV_001045"
     CDS             complement(31412..31891)
                     /locus_tag="ACHOQV_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9629457.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATRFTGMTGAG"
     gene            complement(31906..34353)
                     /locus_tag="ACHOQV_001046"
     CDS             complement(31906..34353)
                     /locus_tag="ACHOQV_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9629458.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAETMAGVKLLMSGYEFAQK
                     NSELQAVLGVAKDSAEMTALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTVASTGKLDKLTAAFKASDGKTSELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIAGGALVLTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFTNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(34346..34501)
                     /locus_tag="ACHOQV_001047"
     CDS             complement(34346..34501)
                     /locus_tag="ACHOQV_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP9629459.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGSTNE"
     gene            complement(34498..34773)
                     /locus_tag="ACHOQV_001048"
     CDS             complement(34498..34773)
                     /locus_tag="ACHOQV_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFP9629460.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(34830..35348)
                     /locus_tag="ACHOQV_001049"
     CDS             complement(34830..35348)
                     /locus_tag="ACHOQV_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP9629461.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATSADAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEVDTGEGKQGEDTESKLPVICTYFKLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(35361..36551)
                     /locus_tag="ACHOQV_001050"
     CDS             complement(35361..36551)
                     /locus_tag="ACHOQV_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFP9629462.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALMGAESVTGVKPRILGVPGLDTKEVAVALASVCQELNAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQKPGTDADLLNEAGVTTLVRRDGFRFWGN
                     RTCSDDPLFLFESYTRTAQVLADTMAEAHMWAIDKPITATLIRDIIDGINAKFRELKN
                     NGYIVDGTCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITSRYLASLVTS
                     VNSN"
     gene            36964..37407
                     /locus_tag="ACHOQV_001051"
     CDS             36964..37407
                     /locus_tag="ACHOQV_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9629463.1"
                     /translation="MLDIRMSRPSEASEIIQIWKSSVDATHDFLTGHDRQEIEKEVIG
                     FFSETPVWVATNQDDQPLGFMFLHEGHLEALFVAGSARGLGVGKRLISHALALHPELS
                     VDVNEQNQQAVGFYQHMGFQVSGRSDLDNQGRPYPLLHLRMANNM"
     gene            complement(37428..38012)
                     /locus_tag="ACHOQV_001052"
     CDS             complement(37428..38012)
                     /locus_tag="ACHOQV_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9629464.1"
                     /translation="MDKKFKNQIVDSDIARLLLKHRKARNLTVTELAQRSGVSQAMIS
                     KIERGISSPSATILSRLANAMNVTLSKLFAELEQHQNSLVRFAHQQQHWTDEQSGITR
                     WSLSPAGACPELIKVEIPAMGELTIPASANEHLSGQTLWMMSGSLDFRVKNQTHHLRA
                     GDCLALTFASEYQMSNPDSEQSCSYIVAFSQKNQ"
     gene            complement(38248..38775)
                     /locus_tag="ACHOQV_001053"
     CDS             complement(38248..38775)
                     /locus_tag="ACHOQV_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9629465.1"
                     /translation="MQHLKNITAGNPKTVAQYQLTKNFDVIWLWSEEGKNWYEEVSNF
                     QEDTIKIVYDENNIIVGITRDASTLNPEGFSVVEVPDITSNRRADDSGKWMFKDGAVI
                     KRIYTADEQEQQAESQKAALLSEAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(38779..40824)
                     /locus_tag="ACHOQV_001054"
     CDS             complement(38779..40824)
                     /locus_tag="ACHOQV_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9629466.1"
                     /translation="MSTKFKTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSMSETLAATPKAVKTVMDETNKKAPLNSPALTGTPTTPTARQGTNNTQ
                     IANTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQPKDATLTA
                     LAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQETVNRAGNAV
                     QKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSYMWFETGDNGNEYFKW
                     RSRQSTTTKDLMNLKWDALYVLVKALFSSEVKISTVNALRIFNSSFGAIFRRSEENLY
                     IIPTRENEGENGDIGSLRPFGINLRTGVVSVGNGARIDGGLALGTNNALGGNSIVLGD
                     NDTGFKQNGDGNLDVYANNVHVMRFVSGSIQSNKTINITGRVNPSDYGNFDSRYVRDI
                     RLGTRVVQTMQKGVMYEKAGHVITGLGIVGEVDGDDPAVFRPIQKYINGTWYNVAQV"
     gene            complement(40835..41365)
                     /locus_tag="ACHOQV_001055"
     CDS             complement(40835..41365)
                     /locus_tag="ACHOQV_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFP9629467.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDSCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWETSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(41358..42266)
                     /locus_tag="ACHOQV_001056"
     CDS             complement(41358..42266)
                     /locus_tag="ACHOQV_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFP9629468.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELLWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(42271..42618)
                     /locus_tag="ACHOQV_001057"
     CDS             complement(42271..42618)
                     /locus_tag="ACHOQV_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFP9629469.1"
                     /translation="MTARYLGMNRSNGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(42615..43250)
                     /locus_tag="ACHOQV_001058"
     CDS             complement(42615..43250)
                     /locus_tag="ACHOQV_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP9629470.1"
                     /translation="MNTLANIQELARALRNMIRTGLVVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITSTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
BASE COUNT         9854 a        11704 c        11328 g        10523 t
ORIGIN      
        1 gttttattat taccactttt aattcaggga taatgtgagg ttataaacct caaataatat
       61 gagataaata gtgctgcaac attgcatttt tgccccgatt tatccatgat cgaattgtga
      121 catttgtcat acaacgaata ggttttgtac ttactatgga atggattgcc gatccgtcta
      181 tctgggccgg gttaatcacg ctgattgtga tcgaactggt cctcggcatt gataacctgg
      241 tctttattgc catcctcgcc gaaaaactac cgccgaagca gcgcgaccgc gcacgggtta
      301 ccgggctgct gctggcgatg ttaatgcgcc tgttactgct ggcgtcaatc tcctggctgg
      361 tcaccctgac tcaaccgctg ttcagcttcc gctcgtttac ctttagcgcc cgcgacttaa
      421 tcatgctgtt tggtggtttc ttcctgttgt tcaaagccac gatggagctg aacgaacggc
      481 tggaagggaa agacagcaat aaccccaccc aacgcaaagg tgcgaagttc tggggggtgg
      541 tgacacaaat tgtggtactg gacgccatct tctcactcga ctcggtgatt accgccgtcg
      601 ggatggtcga ccatttactg gtcatgatgg ccgccgtggt tatcgctatc agcctgatgt
      661 tgatggccag caagccgtta acgcaatttg ttaacagtca cccgaccatc gttattctct
      721 gcctaagttt cctgttgatg attggcttta gcctggtggc agaaggtttc ggcttcgtca
      781 ttccgaaagg ctacctgtac gctgccattg gtttctcggt gatgatcgag gcgctcaatc
      841 agttggctat ctttaaccgg cgacgttttc tttccgctaa ccagacactg cgccagcgga
      901 ctaccgaagc ggtaatgcgc ctgcttagcg ggcaaaaaga agatgcggaa ctggacgccg
      961 aaaccgcgtc tatgctggtg gatcatggta accagcagat ctttaatccg caggaacggc
     1021 ggatgattga gcgggtactt aatcttaacc agcgtaccgt cagcagcatt atgacgtcgc
     1081 gccacgatat tgagcatatc gatctcaacg cgccggaaga agaaatccgc cagttactgg
     1141 agcgaaatca gcatacgcgg cttgtcgtta ctgatggcga tgacgcagaa gatttgctcg
     1201 gtgttgttca cgttatcgac ctgttacaac agtctctgcg cggcgaaccg ctcaacctgc
     1261 gggtgttgat tcgccagccg ctggtgttcc cggaaacctt gccgttgtta cctgccctgg
     1321 agcagttccg taatgcccgc acacactttg cttttgtggt ggatgagttt ggctcggtgg
     1381 aagggattgt gacattaagt gacgtcactg aaaccattgc cggtaactta ccgaacgaag
     1441 tggaagagat cgacgcccgc catgatattc agaagaatgc ggacggttcc tggacggcga
     1501 atggtcatat gccgctggaa gatctggtgc aatatgtgcc gctaccgctg gatgagaaac
     1561 gcgaatatca caccattgcc gggctgttga tggaatattt gcaacgtatt ccaaaacctg
     1621 gcgaagaagt tcaggtgggg gattatttgc ttaaaacgtt gcaggtggaa agccatcgcg
     1681 tgcagaaggt gcagattatt ccgctgcata aggatggcga gatggagtac gaggtgtgat
     1741 aaaacatcaa atgcgcaggt tcaaactgcg catttgattt atacaaaatt tagtataatc
     1801 taaggatgga gtaatgagaa aaaagctggc ttttcttgat accagtcttg acgatttacg
     1861 cgcatttcca gaatcgtctc gacaggaaat tggctatcaa ctcgacagga tccaacaagg
     1921 gttaaaccca tacgactgga aacctttttc aactatcggc cctggtgtgc gagagattcg
     1981 tactcgcgat gctgatggga tctatcgcgt catgtatgtc gcaaaatttg aagaagcggt
     2041 ttacgtattg cattgttttc aaaagaaaac acagactacc agtcaatcga atattgattt
     2101 agccaaacga cgctataaag aacttgttca ggagcggaaa aatgaaaatt gaaacctttg
     2161 atagtgtgtg ggatgcggtg agtgacaccc ctgaacaagc ggaaaatatg cggatccgtg
     2221 ccgaacttgt tacgatcatt aataactgga ttgaacaaca agggtttagc caggctcagg
     2281 cagcgtctgc tttaggggtc actcaacctc gcatatctga acttgccaga ggtaagatcc
     2341 agatctttag tatcgataaa ttgatcacca tgatggctca tgcaggattg catattcaac
     2401 gtattgaagt tcaatatcca catgctgctt agtactataa atacagatgg cgacaaattg
     2461 acgccgccat ctgcataact tgtcttacat cttctccagc aacttcttca catctttgcc
     2521 attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg cttcgtcctg
     2581 tagatgtttg cgcagtaact gatccacttg caggctgtaa ttaagttgct gccagttgcc
     2641 ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc tttccccgtt
     2701 ccaaccgcca atcacacgaa tgtcaaactg ggtgtcgcag gtttgatctg ccagattcaa
     2761 catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt ttaacgtcac
     2821 gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agacgcgtta cgttatcgaa
     2881 gttttcagcg gccttcacat caccaccatt acgttctacc gcttgctgaa tcatctgctg
     2941 gaagttcatc ccttccatgc gcgtgtcggt catttcgaca tgtgcctgtc cttgccagtt
     3001 atggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca gtgacatctt
     3061 tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtgccaatct caacgttttc
     3121 cagccgtggc tggaagttta ttcgcggatt tattgatgtc gcgtccagcg tgcccggcag
     3181 tgaaacctgt ccaccgttaa gtttgccctg cagttgagta atttccagca gacccgactt
     3241 gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca cgttactggc
     3301 ctgcaacaaa atatcagccg taaagccttg cagtccctga taggccggtt catcaatacg
     3361 cgaagaaatg accgggcgcg gcaacgtgga ttggctctgc ccctgctgcg cggcaccgtt
     3421 ttcaccgttc gctgtttctt taagcgggat gaggttgtca agattcagtt gcgggaattg
     3481 cagcctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgcc cgctcagcgt
     3541 actgtcattg gcggtcaagc tgatttggtt aaatgaaagg cgtttatgtg actcctgcca
     3601 ctgggcctgg aaactcccct gcccctgaat accttgtttt ggtaaatctg caccctgcaa
     3661 ctgccagtta acttgttcaa tagccgccgt taaatcatgc ggataatcag aagcatctac
     3721 cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc cggagaactc
     3781 aaatgaacca cgatgttggg gatcttgttc catttgcagg cgaatattgc ggattgtcac
     3841 ctgctcgtca tcttcatgct ggaacaccag cacgctgtcc gccaccttaa gactggatat
     3901 atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc gcggtgcaac
     3961 cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca ctgccccttt
     4021 tagcatcacc tgcttaacgc tcagttgatg actcagcagt ggtaaaagcg ccacgtccag
     4081 acgcatgttg tctgcgcgaa ccagtggctg gctcgcgccc tgggcagtga gagacattcg
     4141 cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt cgagctgcaa
     4201 ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat cattcggatt
     4261 caccagcaac actaacgcag ataacccggc caccagcacg accaggagta tcatcagcgt
     4321 cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat cgattcgact
     4381 ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt aaggtcgcgc
     4441 cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg gacgcagcgc
     4501 cagcggcagc ttaccggagt tgtagaactc cagcacaata cagccagacc agcccggatc
     4561 gatacggtgc gcggtgacgt gcaccataag ccccagacgc gccagtgagg aacgaccgtc
     4621 cagccatccc accagatcgg ctggcagcgt caccgactcc agcgtcaccg ccagcgccag
     4681 ctctcctggg tgaagataaa acgcctcgct ttcatcgagt acgatctcat cgctcatcac
     4741 gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa acgcagccgt
     4801 gtgaccacgg aaggtacgaa atttattgcc caggcgcaca tccaccgtcg cgccgttaat
     4861 acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc aggcttcaat
     4921 atctcggtca cacagacgca tggcattttc tcctttcgcg catcactccc ttaacgccaa
     4981 ttacgtcaag ggcataccag gttatcactg aacggtacac aatttatcaa gcttattcaa
     5041 agaactgact tattttcgct ttcaatatat cgatggcgat gcggtttttc ccgccgcgcg
     5101 gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac atcggacgca
     5161 cggttttttg atattgcgcc atcactgaat ccattgaacg cccacgctcg ttaacgtcac
     5221 gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg gagaagttaa
     5281 gttcatcacg caaacgcgca tccgtcagca gcaaaatgcc ttcgagaatg atgactttct
     5341 tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg taaaccggca
     5401 ggtcaatggc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg ctgtgatcca
     5461 tagcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga tggctttgat
     5521 ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact tgctcacgta
     5581 attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg ccagcgatac
     5641 cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga ttaacctgga
     5701 tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc atccgcctga
     5761 taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacgaaga attcccggtg
     5821 gcaaattacg ttgatcagtt ttatacaagg taaaaattgt tatacgcagt tgcgcaaatt
     5881 atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact tttgtactag
     5941 cataaacaca gaaacgaata ctggcgacct ggtcttgcgg ataaagcggt aatgagcaaa
     6001 caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt cagtttaggt
     6061 ttagtctcgt ttatctttac ccttttctcg cttgagcttt cgcagtttgg cacccaactc
     6121 gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca tgccgggcgc
     6181 atgtggccgg ggattgcgct gagctgctcg ctgggaaata tcgccgcatc catcctgctt
     6241 ttttccacca gctcgctgaa catgacctgg acgaccatca atattgttga agccgtggtc
     6301 ggggcagtgc tactgcgtaa attgctgccg tggtataacc cattgcaaaa tatggctgac
     6361 tggctgcgtc tggcactcgg cagcgccatt gttccacctc tgttaggggg tgttctggtt
     6421 atcctgctga cgcccggaga cgatcctctc agggcatttt tgatatgggt actgtcagaa
     6481 tccatcggcg cactggcact ggtgccgctg ggattgttat ttaaaccaca ctatctgctg
     6541 cgccatcgca acccacggtt gctttttgag tcgctgctca cgttagccat cacactgacg
     6601 ttaagctggc tttcgatgct gtatctgccg tggcctttta ctttcattat tgtgctgttg
     6661 atgtggagcg ccgtgcgcct gccacgaatg gaagcctttt tgatcttcct taccacggtg
     6721 atgatggtgt cactgatgat ggccgcggat ccctccctgc ttgctacgcc gcgtacatac
     6781 ctgatgagcc atatgccgtg gctaccgttt ttgctgatcc tgctgcccgc caacatcatg
     6841 accatggtga tgtatgcctt tcgtgcggaa cgcaaacaca tttccgaaag cgaaacccgc
     6901 tttcggaacg cgatggaata ttccgccatc ggcatggcat tagtgggcac cgagggacaa
     6961 tggctgcaat ccaacaaagc gctctgccag tttctcggtt acagtcagga agagctgcgc
     7021 ggactcacct ttcagcaact gacctggccg gaggatctca ataaagatct gcaacaggtt
     7081 gaaaagctga tcagcggtga aataaacacc tattcaatgg aaaaacgcta ctacaaccgc
     7141 aatggcgatg ttgtctgggc gttgcttgcc gtctcactgg tgcgccacac cgatggtacg
     7201 ccgctctatt ttatcgctca gattgaagac attaacgagc taaaacgcac cgaacaggtg
     7261 aaccagcaac tgatggagcg catcacgctg gctaacgaag cgggcgggat tggcatctgg
     7321 gagtgggagc taaagccgaa tatttttagc tgggataagc ggatgttcga gctgtatgaa
     7381 attcctccgc atatcaaacc gaactggcag gtgtggtacg agtgcgtact gccggaagat
     7441 cgccagcatg ccgaaaaagt gattcgtgat tcgttgcaat cacgctcgcc ctttaagctg
     7501 gaatttcgca ttgccgtgaa agatggcatc cgccatatcc gcgccctcgc caaccgggta
     7561 ctgaataaag aaggcgaagt cgaacgcctg ctcggcatta atatggatat gaccgaagtg
     7621 aaacagctta acgaggcatt gtttcaggaa aaagagcgcc tgcacattac gctggattcc
     7681 atcggcgaag ccgtggtctg tattgatatg gcaatgaaaa ttacctttat gaatccggtg
     7741 gcggagaaga tgagcggctg gacgcaggaa gaagcgttag gtgttccgct cctgacggtg
     7801 ttgcatatta cttttggcga caacggacca ttaatggaga acatttacag tgccgacacc
     7861 tcacgttccg cgattgaaca agatgtggtg ttgcactgtc ggagcggcgg cagctacgac
     7921 gtgcattaca gtattacgcc gttaagtact ctggacggca gcaatatagg ctcggttctg
     7981 gtgattcagg acgtcaccga atcgcgtaaa atgctgcgcc agctgagcta cagcgcctcc
     8041 catgatgcac tgacgcatct cgccaatcgc gccagttttg agaagcaact acgcatcctg
     8101 ctgcaaacgg taaacagtac acatcagcga catgcactgg tgtttatcga tcttgatcgc
     8161 tttaaagcgg tgaatgacag cgccgggcac gccgctggcg acgctttact gcgcgaactg
     8221 gcgtcattga tgctgagtat gctgcgctcc agcgacgtgc tggcgcgact cggtggcgat
     8281 gaatttggtc tgctgctgcc agattgtaat gtcgaaagtg cgcgttttat cgctacacgc
     8341 attatcagcg ccgtgaatga ctatcacttt atctgggaag gacgtgtaca tcgggtaggt
     8401 gccagtgccg ggattacctt gattgatgac aacaatcatc aggcggctga agtgatgtcg
     8461 caggctgata tcgcctgtta tgcctccaaa aatggtggac ggggccgggt gacggtttac
     8521 gaaccgcagc aagctgccgc acatagcgaa cgggcggtga tgtcgcttga tgaacagtgg
     8581 cggatgatta aagagaatca gttgatgatg atcgcccacg gtgtcgcttc gccgcggatc
     8641 ccggaagcgc gtaatttgtg gctgatttca cttaagctct ggagttgcga aggcgagatt
     8701 attgatgaac aaacatttcg tcgtagcttc agcgatccgg cacttagcca tgctcttgac
     8761 cgacgggtat tccacgattt tttccagcag gccgcaaaag cgattgccag taaaggctta
     8821 agcatcgccc tccccctttc cgttgccggt ttgagtagcg ccacgctggt gaatgaactg
     8881 cttgagcagc tggaaaatag ccctctaccc ccacggttat tacatctgat tattccggca
     8941 gacgcgattt tagatcacgc agaaagcgtg caaaaactgc ggctggcggg atgtcggatc
     9001 gtattcagtc aggtgggccg cgatctgcaa atcttcaact cgctgaaagc gaatatggca
     9061 gattacctgc tacttgatgg tgagttatgc gccaatgtgc agggaaattt gatggatgag
     9121 atgctgatta cgatcattca ggggcacgct cagcggctcg ggatgaagac catcgccggg
     9181 ccagtagttt tacccctggt gatggatacg ctttctggca tcggcgtcga tctgatttat
     9241 ggcgatgtga ttgccgatgc ccaaccgctg gatttgctgg tgaatagcag ttatttcgcg
     9301 attaactgag gcgtgcttct ccatcgcctg atacgacgct aacgcgtctt atcatgccta
     9361 caaatcgctc attccccagg ccggataagg cgctcgcacc gcatccggcg accaacgtca
     9421 tgcttcgtct ggttgccagc cttccgtata ccagatatgc aacagcgcat aagaacgcaa
     9481 gggcttccag cgttcggcat aacggcggat ttgtgccggt gtcattcccg gaaatcgctg
     9541 tttaatcaga taatcatccg gcaggaaaac atctttcgcc tgccagccgc gcaaggcgaa
     9601 ataattcgcc gtccagcgcc cgatacccgg aaaagtttgc agcgttttca tcgcctgctc
     9661 cacatcgccc ggtattgtca ttggtaaggt gccctccagc gccgcatttg ccagatgaat
     9721 cagcgcctct gcccgtttca acggcatacc taacgctttt aatgcctgcg gatctgctgc
     9781 tgccagccgc tgaggcgtgg ggaagcagac ataatccgga aaatcatcca gcctttcgcc
     9841 ataaagctgt gccactctgg cggtcaattt tgccgccatc gccacgctca ccagctggcc
     9901 taaaatcgcc cgcacgccct gctcaaaagc atcaacacaa ccgggtaaac gcaatccggg
     9961 ccgtgccgcg cctaacttgc ccaacgcacc gttaacaatc tgcgggttac attgcagatc
    10021 aaacaggcgg ctcattttcg ccagacactc tgcggcaacg ggttctaaac ctgcacttaa
    10081 atttatgtgc agagtatggc tggctatatc cggaatagca gtcaccacgc cgcgacattc
    10141 gcccaccgcc agactacggg cataataatc gtcagcgacc gtttccacac cgttcaccgc
    10201 acgggcggcg agaaatccca acatccacga ccagtcatac ggcggctgcc agttcagggt
    10261 atacatcgca tctccttgtt aatccgcttt cagcataaac gttattcaga cgctacgctt
    10321 tgctttcata ttccggttgt cgcgacggca acatttcgct aaagtcacgc cccttcttca
    10381 ccggcatggg gattatttcg tgtttattgg ttttgattac ggtacagcaa actgttcagt
    10441 ggcggtcatg cgcgacggta agccgcaatt actgaaaatg gaaaacgaca gcacgctgct
    10501 gccttcaatg ctttgcgcgc caacgcgtga agcggtaagc gaatggctgt accgccatca
    10561 tgatgttccg gcagacgacg atgaaacgca ggcgctgttg cgtcgggcga ttcgttataa
    10621 ccgcgaagaa gatatcgatg ttacggcgaa aagcgtgcag ttcggtcttt cctcactggc
    10681 acagtacatt gatgatccag aagaggtgtg gtttgtgaaa tcaccaaaat cattcctcgg
    10741 tgccagcggc ttaaaaccgc agcaggtagc gctgtttgag gatctggtct gcgcaatgat
    10801 gttgcacatt cgccagcagg cgcaggcgca gctgccggag gcaattactc aggcggtgat
    10861 tggtcgcccg atcaacttcc aggggctggg cggcgatgaa gcaaacgccc aggcgcaagg
    10921 gattctggaa cgcgcggcga agcgtgccgg gtttaaggat gtggtattcc agtacgagcc
    10981 ggtcgcggct gggctggatt acgaagccac cttgcaggaa gaaaaacggg tgctggtggt
    11041 ggatatcggc ggtggtacga ctgactgttc attgctgctg atggggccgc agtggcgttc
    11101 gcgtctcgat cgtgaagcca gcctgctggg tcacagtggt tgccgtattg gcggtaacga
    11161 tctggatatc gcactggcgt ttaaaaacct gatgccgtta ctgggcatgg gtggcgaaac
    11221 cgaaaaaggc atcgccctgc cgattctgcc gtggtggaat gcggttgcca tcaacgacgt
    11281 tcctgcgcaa agtgatttct acagtagtgc caacggtcgt ctgcttaacg acctggtacg
    11341 cgatgcccgc gaaccggaaa aagtggccct gttacagaaa gtctggcgtc agcgtttaag
    11401 ctatcgcctg gtacgtagcg cagaagagag caaaattgcg ctttcaagcg tagcggaaac
    11461 ccgcgcctca ctgccgttta tcagcgatga actggctacg ctgattagcc agcaagggct
    11521 ggaaagcgcc ctcagccagc cactggcgcg gattctggaa caggtgcaac tggcgctgga
    11581 taacgcccag gaaaaaccgg atgttatcta tctgaccggc ggtagcgccc gttcaccgct
    11641 gattaaaaaa gcgctgacag aacagttgcc gggcattccg attgcaggtg gcgatgactt
    11701 tggctccgtc accgccgggc tggcacgctg ggcggaagtg gtgtttcgtt aattgaaaac
    11761 tggctcagca acggcttaaa tcagcctgcg cctgagccag tttttctgcc gcctgacgta
    11821 gcggttccat caacggcatg gtccgtgctg gagcatgaag gcggaatcgc tgcaactctg
    11881 tcgcaccgac attcagcgcc gattccagcc gatgtcgttt ggcggccatt tttgccatta
    11941 ccgcttgtct gtctgcaggc gttaccgctt cgttcggcct gaacacaaaa cggcgttcac
    12001 agctcgcttt ccagtcgata accgcctggg tcagatgatc accaaaacct ttcacttgct
    12061 taacgctccg tcgggtaaca tccgctgctg tttcaatacc aaaggaccgt aacgccgctt
    12121 tacgcgcagg gccaacgccg ggaatagagg caacatcaat aaaaaatccc tccagaaact
    12181 tctgcttctg ccgttccctt gcggtgtcct gaagtgctgc cagatcgcgc ttttcttctt
    12241 ccggtaaccc aagaatttcg tccttcattt tttcgagcat cgtccgtttg gcgataaaac
    12301 cttccagccc gcccaactgc tggatctggc tgactaaatg gtcatagtcc attttcgcgc
    12361 gattaaacgc ctcacggcgt tgctggattt ctgctttgta cgctttgctt gtcaggctgc
    12421 caataatcca gatagccgcc agcacaggaa ccaaaataat ataacgtggt tctgtctgaa
    12481 ggccgcaaag cagcagcgat aacgctgaca gtgcgatctc aatcagaatg atgtattcac
    12541 gccttaacag gcctaaagga agcggcctgc cagtcggttg gaaatgatcg ggtaatggta
    12601 attgcaatgc tggcggtgct actgacgcca tcaccatcgc ccagactttc gccagcacaa
    12661 aatcaccgct ggtggtaatg acctcttcgc cgagatcaat aaaatagata acgccttgat
    12721 tatccagagc acaccacggg cagtcagcca aatgaccggg ataaacatgc attgccgaaa
    12781 cggtacattt ctttaactgt tggcgtagta aatcaagtgc cgctacccac gcttttgccg
    12841 tcggacgccc ggttgccacg ccactttccg tgaatgcctg ctgaaacatg gcttcaacat
    12901 gcccggtaac atcgacagcg gaatagatcg tggcggcggt tttaaaccac gtcgctgatt
    12961 atctgacgcg taggcataac ggaaatgggc aatatccgtc tccagtgcat tacccgcatc
    13021 agagacaagc ggtacaccgg aataaggatg ccgcccacca aacaagacgt gaaaaatcag
    13081 caacgcaagg ccaaaattat cgtgattcgc ggtgcgttca aagccgacaa atgatggcaa
    13141 ggtttgttgc tctggcggcg taaaatgcga cacgccgact tcgcataaat gcagcgtgcc
    13201 attggcgtta atctgaaagg agtcgctatc aatcaacacc actttgctgt cgcgacctac
    13261 cataaagctg ttctggttta cgtcacccac gacgtgcccg tgctcgtgaa tcgtagcaaa
    13321 agatgaagca atattgcgcg caacatagag tagaaaatcc cacgcacaat gagggtaacg
    13381 ttgacgacga tgtgccgggc tatagatcat atgaatcggt tctttaccag aaacttttgg
    13441 catcataaaa ccgattactt ttccgcctcg tccaccgtga agcgttgcct gcggccaggc
    13501 gacataattc aacaactgcg cgtcagctgt cgcagccata aaggcaagtt tgtcctgttt
    13561 taaggcgggt ggcggcgtgt gataaatctt ggcgacgcta tcgacaaact cctcgatatc
    13621 ataaaccgcg ccttcaccgc ctttgcccag ttcacagcct ggcgtaacgc actcaccagt
    13681 agcagtatat aaagtgggtt tcatggtaac cacagcgcca gtgccaacgt tttatcatca
    13741 tcggtacgct cgtttaccgc cgggccggac aaaaactgct ttaacaattc aggtaataaa
    13801 tcgagttgtt cctgcgttgc tgacgccagc ccattgaaaa acggggtaaa gaaaggcaca
    13861 tgcggagaat tatccagcat atttaacgcc agccgctgga taccatccgt aaatgctgca
    13921 actttgtgcg cacgctcagt gctggtgaaa gtttccagtc tggaaacggc atcttcatcg
    13981 gtaatgaagt gcgtcatatt ggtatattca cccaccatcg gcgtgagcgg cagttgcaaa
    14041 ccgtgcccca aatctaccac cacgccgcca tcgccaatct gcataatcaa ggtgccatta
    14101 gctgatgaaa tcaatcccaa aaacgtgcag gcaaaatcgc gaaccgctaa ttctttggct
    14161 tccgcctcgg caaaaagtcg ctgacgaatg gtcagcacaa tgtccgttgc gagaacatca
    14221 ttaagcccca attccccgcc ctgcactttt tgcgacatat aagccatcgc ttcattgacg
    14281 gcgagcatcg caccttcgcc tccctgcgag gcgctacctg cgccatcagc gacaaatacc
    14341 gacaataatg gctgctggtc atttaaccag gcaacctgca tctggcaggc atcctggcaa
    14401 ggcaagtccg cgctgatgtg cgacgttcca acggttgatg catagaccag acgccagctc
    14461 acactgacgt ccagcctttt ggcgcttcca gcacaacttc cgttcccggc gtggagcggg
    14521 aaaccgaacg cagcgaactg gataaccagc taaatagttc gcggaattgt aatccttgaa
    14581 gaggcaaagg ctgacgaacg ctgatttggg ccagcgtctt catatccgcc ccctgaacac
    14641 cgatggaaaa gaaggcaaac ttcttatctt cttccccctg aaacaccttg ttggcagcag
    14701 cctgccactc atcggttggt gcgccatcgg taatcaggaa aatccacgga cgataatagg
    14761 aaataccatt ggcgcgatac tcacgtttgc gctcctcaac catatccagg gctttggtaa
    14821 tggcggcccc cattggtgta tcgccctggg caaacaggat gggcgggaaa aaattagcgg
    14881 cgctggtaaa tggttgttcc acatgcaccg ggccgaacgt cacaatgccg agttccactc
    14941 tttttaaggc cagcggatcg gcaagcagct catcgcgaaa ggtaaccaat ccggcgttaa
    15001 gttcattaat aggtctaccg ttcatagaac cagaaacatc caacagcaaa atgcaggggc
    15061 aacgtggttc cgggttactg gcaaaatcgc tggtggcaaa cgtgatttgt tcgctcataa
    15121 gaaatccttt cttagagaat tattgaggcc cggtatcata ccgactgcaa caatgaaata
    15181 agagcaaacg aagaaacttt cagaattagg cccggcggcg cagaaaattt tgctattcgc
    15241 ctcgcaagaa cggagagccg tcgattgaca ctaagggcgg agtgacataa tttcaggagt
    15301 gagggttagg gagaggtttc cccctccccc tggtgttctt agtaagcctg gaagctaatc
    15361 actaagagta tcaccagtat gatgacgtgc ttcatcataa ccctttcctt attaaaagcc
    15421 ctcttctccg ggagagggtt tcccgtttca gcgtcccgct gaaatcgtcg gcttacctcc
    15481 tttcgccctg aatgcagtct atcgctaacg cgtgaacaaa atagtttcct ctgtgattat
    15541 tgcgtaacga ctcgcaaaga atatttgctg gcaggatcgc agactacaaa gcctgcggat
    15601 tgacaatctt atcgtgaagg cataatttca ggagtgaggg tagagcgggg tttcccccgc
    15661 cctggtagtc ttagtaagct ggccagctaa taactaagag caccacgatg atgagtagct
    15721 tcatcatgac cctttcctta tttaaggccc cttcctcggg aggggctttc ccgtttcagc
    15781 gtcccgctga aatcggcggc ctacctcctt tcgccatgca agcagtctat cgctaacttg
    15841 cagataaaat agtttcacat gtgattattg cgtaacgtct cgcaaagaat atttgctggc
    15901 aggatcgcag actacaaagc ctgcggattg acaatctaat tgtgaaggca tactttcagg
    15961 agtgagggta gagcggggtt tcccccgccc tggtagtctt agtaagccgg gaagcttatg
    16021 actaagagca ccacgatgat gatccacttc atcatgaccc tttccttatt taaggcccct
    16081 tcctcgggag gggctttccc gtttcagcgt cccgctgaaa tcatcggctt acctcctttc
    16141 gccctgaatg cagtctatcg ctgacttgcg gataaaatag tttcacatgt gattattgcg
    16201 tgacgtctcg caaagaatat ttgctggcag gatcgcagac tgcaaagcct gcggattgac
    16261 aatcggaccg tgaaggcata ctttcaggag tgagggtaga gcggggtttc ccccgccctg
    16321 gtagtcttag taagctgggt agctaacgac taagagcacc acgatgatga gtatcttcat
    16381 catgaccctt tccttattta aggccccttc ctcgggaggg gcttgcccgt ttcagcgtcc
    16441 cgctgaaatc gtcggcctac ctccttttgc cctgaatgca gtctatcgct aacgcgtaga
    16501 taaaatagtt tcctgtgtta ttactggatg ggtgctcgca aatgtgcccg ccattcagac
    16561 gattcccgat agtgtttcat aattcctcca tttttctccc tgattggctg gctacactag
    16621 tatcattccg cgaatcgttt caggaagaga aactcttaac gatgaaaagc agttataaat
    16681 cccgttgggt aatggtaatc gtggtggtta tcgccgccat cgccgcattc tggttctggc
    16741 aaggccgcaa tgactcccgg agtgcagcgc caggggcgac gaaacaagcg cagcaatcgc
    16801 cagcgggtgg tcgccgtggt atgcgttccg gcccattagc cccggttcag gcggcgaccg
    16861 ccgtagaaca ggcagttccg cgttacctca ccgggcttgg caccattacc gccgctaata
    16921 ctgttacggt gcgcagccgc gtggacggtc aactgatggc gttgcatttc caggaaggcc
    16981 agcaggtcaa agcaggcgat ttactggcag aaattgaccc cagccagttc aaagttgcat
    17041 tagcacaagc ccagggccaa ctggcaaaag ataaagccac gcttgccaac gcccgccgtg
    17101 acctggcgcg ttatcaacaa ctggtaaaaa ccaatctcgt atctcgtcag gagctggatg
    17161 cccaacaggc gctggtcagt gaaaccgaag gcaccattaa ggctgatgaa gcaagcgtcg
    17221 ccagcgcgca gctgcaactc gactggagcc gcatcaccgc accagtcgat ggtcgcgttg
    17281 gtctcaagca ggttgatgtt ggtaaccaaa tctccagtgg tgataccacc gggattgtgg
    17341 tgatcaccca gacgcatcct atcgatttgc tctttaccct gccggaaagc gatatcgcta
    17401 ccgtagtgca ggcgcaaaaa gccggaaaac cgctggttgt tgaagcctgg gatcgcacca
    17461 attcaaagaa attaagtgaa ggcacgctgt taagtctcga taaccaaatc gatgccacta
    17521 ccggtacgat taaagtgaaa gcacgcttca ataatcagga tgatgcgctg tttcccaatc
    17581 agtttgttaa cgcgcgcatg ttagtcgaca cagagcaaaa cgctgtggtg atcccaacag
    17641 ccgccctgca aatgggcaat gaaggccatt ttgtctgggt gctgaatagc gaaaacaagg
    17701 tcagcaaaca tctggtgacg ccgggcattc aggacagtca gaaagtggtg atccgcgcag
    17761 gtatttctgc gggcgaccgc gtagtgacag acggcattga tcgcctgacc gaaggggcga
    17821 aagtggaagt agtggaagcc cagagcgcca ccactccgga agagaaagcc accagccgcg
    17881 aatacgcgaa aaaaggagca cgctcctgat gcaggtgtta cccccgagca gcacaggcgg
    17941 cccgtcgcgc ctgtttatta tgcgtcctgt cgccaccacg ctgctgatgg tggcgatctt
    18001 actcgccggg attatcggtt atcgagccct gcccgtttcg gcgctgccgg aagtggacta
    18061 tccgaccatt caggtagtca cgctctaccc aggtgcgagc ccggatgtca tgacctctgc
    18121 cgttaccgcg ccgcttgaac gccagtttgg gcagatgtct ggcctgaaac agatgtcgtc
    18181 gcaaagttcc ggcggtgcgt cagttatcac tttgcagttc cagctaacat taccgctcga
    18241 tgtcgccgag caggaagtgc aggccgcgat taacgctgcg accaacttgt tgccgagcga
    18301 tctgcctaac ccgccggttt acagcaaagt gaacccggca gatccgccga tcatgacgct
    18361 cgctgtcacc tcaaccgcca tgccgatgac gcaagtggaa gatatggtgg aaacccgcgt
    18421 cgcgcagaaa atctcgcaga tttccggcgt cggcctggtg acactttccg gcggtcagcg
    18481 tccggctgtt cgcgtcaaac ttaacgctca ggcgattgcc gccctcggcc tgaccagcga
    18541 aaccgtgcgc accgccatta ccggcgctaa cgtcaactcg gcaaaaggta gcctcgacgg
    18601 cccttctcgt gcggttacgc tttccgctaa cgaccagatg caatccgccg aagagtatcg
    18661 ccagctaatc atcgcctacc agaacggcgc gccgattcgt ctgggcgatg tcgcaaccgt
    18721 agagcaaggt gcagaaaaca gctggcttgg cgcatgggct aacaaagaac aggccattgt
    18781 gatgaatgtt cagcgccagc ccggtgctaa cattatctcc accgccgaca gcattcggca
    18841 gatgctgcca cagctcaccg agagtctgcc gaaatcggtg aaggtgacgg tactttctga
    18901 ccgtaccacc aatattcgcg catccgtgga tgatactcag tttgaattga tgatggctat
    18961 cgcgctggta gtcatgatta tctacctgtt tttgcgtaat attccggcga ccatcattcc
    19021 cggcgtcgca gtgccgctgt cgttaatcgg caccttcgcg gtcatggtgt ttctcgattt
    19081 ttcaatcaat aacctgacgc tgatggcgtt aactatcgcc accggatttg tggtcgatga
    19141 cgccatcgtg gtgatcgaaa acatttcccg ctatatcgaa aaaggcgaaa aaccgctggc
    19201 ggcggcgctc aaaggcgcag gtgaaatcgg ttttaccatt atctcgctga ccttctcgct
    19261 gattgcagtg ctgatcccgc tactgtttat gggcgatatc gtcgggcgac tgttccgcga
    19321 atttgctatt actctggcag tcgcgatttt gatctcagcc gtggtgtcgc tgaccctgac
    19381 accgatgatg tgtgcgcgga tgctcagcca ggagtcgctg cgtaaacaga accgcttctc
    19441 ccgtgcctcg gaaaaaatgt tcgacaggat aatcgccgcc tatggtcagg gacttgcgaa
    19501 agtgctgaat catccgtggc tgaccttaag cgtggcgctc agcacgctgc tgcttagcgt
    19561 gctgttgtgg gtgttcattc cgaaaggttt cttcccggta caggacaacg gcattattca
    19621 gggcactttg caggcaccgc aatccagctc ctttaccaat atggcccagc ggcaacgcca
    19681 ggtcgcggat gtgattttgc aagatccggc ggtgcaaagt ctgacctcat ttgttggcgt
    19741 tgatggcact aacccgtcgc tgaacagtgc gcgtttgcag atcaacctca aaccgttgga
    19801 tgaacgtgat gatcgggtgc aaaaagtcat cgcccgcctg caaacggcgg tggataaagt
    19861 gccgggcgtc gatctcttcc tgcaaccaac gcaggacctg accatcgata ctcaggtcag
    19921 ccgcacccag taccagttta ccttgcaggc cacgtcactg gatgcgctca gtacctgggt
    19981 gccacagttg atggaaaaac tccagcaact gccacagctt tctgatgtct ccagcgactg
    20041 gcaggacaaa gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg
    20101 gatcagcatg gcggacgtcg ataacgccct ttacaacgcg tttggtcagc gactgatttc
    20161 cactatttat actcaggcca accagtatcg cgtggtgctg gagcacaaca ccgaaaatac
    20221 tcctgggctt gcggcgctgg atactattcg cctgaccagc agcgacggcg gcgtggtacc
    20281 gctaagctca attgccaaaa ttgagcagcg ttttgcgccg ctttccatca accatctgga
    20341 tcagttcccg gtaacgacca tctcctttaa cgtgccggat aactattcgc tgggcgatgc
    20401 ggtgcaggcg attatggaca ccgaaaagac gctgaatctg ccggtggata tcaccacgca
    20461 gttccagggc agcaccctcg ccttccagtc ggcgttgggc agcactgtct ggctgattgt
    20521 cgcggcggtg gtggcgatgt atatcgtgct cggcattctg tacgagagct ttattcaccc
    20581 gatcaccatt ctctcgacgc tacccaccgc aggggtcggc gcactgctgg cgttactgat
    20641 tgccggtagc gagctggatg tgattgcgat tatcggcatt attttgctga tcggtatcgt
    20701 gaagaagaac gccatcatga tgatcgactt cgcgctggct gctgagcgcg agcaaggcat
    20761 gtcgccgcgc gatgcaatct accaggcttg tctgttgcgt tttcgtccga tcctgatgac
    20821 caccctggcg gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga
    20881 actgcgtcgt ccgttaggta tcggcatggt cggcggtctg attgtcagcc aggtgctgac
    20941 gctgttcacc acgccggtga tttatctgct attcgaccgc ctggcattgt ggaccaaaag
    21001 ccgctttgct cgtcatgaag aggaggcgta agtgaagttt tttgccctct tcatttaccg
    21061 cccggtggcg acgattttac tgtcggtcgc cattaccctg tgcggcatac tgggtttccg
    21121 tatgctgcca gtcgccccgc tgccgcaggt cgattttccg gtgattatgg tcagcgcctc
    21181 gctgcccggt gcgtcaccag aaacaatggc gtcttccgtt gccacgccgc tggaacgttc
    21241 gcttggacgc attgccgggg tcagtgaaat gacctccagc agttcgctcg gcagcacgcg
    21301 cattattttg cagtttgatt ttgaccgcga tatcaacggc gcagcgcgcg atgtgcaagc
    21361 ggcgatcaac gccgcgcaaa gtttgctgcc cagcgggatg ccaagccgtc cgacctatcg
    21421 caaagcgaac ccgtcggatg cgccaattat gatcctcacg ctgacatccg atacttattc
    21481 gcagggtgaa ctgtacgatt tcgcctcgac gcagctggct ccgacgattt cgcaaatcga
    21541 cggtgtcggt gatgtcgatg ttggcggcag ctcactgccc gccgtacgca tcgggctgaa
    21601 tccgcaggcg ctgtttaatc agggcgtgtc gctggacgat gtgcgcagcg ccatcagcaa
    21661 tgccaacgtg cgtaagccgc agggggcgct ggaagatgat acccatcgct ggcaggtcca
    21721 gactaacgat gaactgaaaa ccgccgccga atatcagccg ttgattattc attacaacaa
    21781 cggcggcgcg cttcgtctgg gcgatgtggc gacggtgacc gactcggtgc aggatgtgcg
    21841 caacgccggg atgaccaacg ccaaaccggc tattttgctg atgatccgca agctgccgga
    21901 agccaatatt atccagacgg ttgacagcat ccgggcaaaa ttaccggagt tgcaggagac
    21961 cattccggcg gcgattgatc tgcaaattgc ccaggatcgc tcccccacca ttcgcgcctc
    22021 gctggaggaa gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc tggtggtctt
    22081 tttattcctg cgctcgggtc gcgccactat tattcccgcc gttgcggtgc cggtttcgct
    22141 gattggtacg tttgcggcga tgtacctgtg cggattcagt ctcaataacc tttcgttaat
    22201 ggcgctcacc atcgctactg gtttcgtggt ggatgacgcc atcgtggtgc tggaaaacat
    22261 tgcccgtcac ctggaagcgg ggatgaaacc gctacaggcc gcgctgcaag gcactcgcga
    22321 agtcggtttt acggtgctgt cgatgagtct gtcactggtg gcggtgtttc tgccgctgct
    22381 gttgatgggc ggattgccgg gtcgcctgtt acgcgagttt gccgtgacgc tttctgtcgc
    22441 cattggtatt tcgctgctgg tttctctgac gctaacgcca atgatgtgcg gctggatgct
    22501 aaaagccagt aagccgcgcg agcaaaagcg actacgtggt tttggtcgca tgttggtagc
    22561 cctgcaacaa ggctatggta agtcgctgaa atgggtgctt aatcataccc gtctggtggg
    22621 cgtggtgctg cttggcacca ttgctttgaa tatctggctg tatatctcga tcccgaaaac
    22681 cttcttcccg gagcaggaca ccggcgtgtt gatgggcggg atacaggcgg atcagagtat
    22741 ttcgtttcag gcgatgcgcg gtaagttgca ggattttatg aaaattatcc gtgacgatcc
    22801 ggcagtggat aatgtcaccg gatttaccgg cggttcgcgg gtgaacagcg ggatgatgtt
    22861 tatcacgctg aagccacgcg gcgaacgcag cgaaacggcg cagcaaatta tcgaccgtct
    22921 gcgcgtgaag ctggcgaaag aaccgggggc gaatctgttc ctgatggcgg tacaggatat
    22981 tcgcgttggc ggacgtcagt cgaacgccag ctatcagtac acgttgctgt ccgacgacct
    23041 ggcggcactg cgcgaatggg agccgaaaat tcgcaaaaaa ctggcgacat tgccggaact
    23101 ggcggacgtg aactccgacc agcaggataa cggcgcggag atgaatctgg tttacgaccg
    23161 cgacaccatg gcacggctgg gaatcgacgt gcaggccgcc aacagcctgt taaataacgc
    23221 cttcggtcag cgacaaatct cgaccattta ccagccgatg aaccagtata aagtggtgat
    23281 ggaagtggat ccgcgctata cccaggacat cagtgcgctg gaaaaaatgt tcgttatcaa
    23341 taacgaagga aaagcgatcc cgctgtcgta tttcgctaaa tggcaaccgg cgaatgctcc
    23401 gctgtcggtg aatcatcagg gattatcggc agcctcgacc atctcgttca acctgccgac
    23461 cgggaaatcg ctctcggacg ccagtgcggc gatcgatcgc gcaatgaccc agcttggtgt
    23521 gccttcgacg gtgcgcggta gttttgccgg cacggcgcag gtgttccagg agacgatgaa
    23581 ctcgcaggtg atcctgatta tcgccgccat cgccacggtg tatatcgtgc tgggtatcct
    23641 ttacgagagt tacgtacatc cgctgacgat tctctccacc ctgccctcgg cgggcgttgg
    23701 agcgctgctg gcgctggagc tgttcaatgc cccgttcagc ctaatcgccc tgatagggat
    23761 catgctatta atcggcatcg tgaagaaaaa cgccattatg atggtcgatt ttgcgcttga
    23821 agcccaacgg cacggtaacc tcacgccgca ggaagctatt ttccaggcct gtctgctgcg
    23881 ttttcgcccg attatgatga ctaccctggc ggcgctgttt ggcgcgctgc cgctggtatt
    23941 gtcgggcggt gacggttcgg agctgcggca acccctgggg atcaccattg tcggcggtct
    24001 ggtcatgagc cagcttctga cgctgtatac cacgccggtg gtgtatctct ttttcgaccg
    24061 tctgcgactg cgtttttcgc gtaaacctaa acaagcggta accgagtaaa tgacagatct
    24121 tcccgacagc acccgctggc aattgtggat tgtggctttc ggcttcttta tgcagtcgct
    24181 ggacaccacc atcgttaaca ccgcgcttcc ctcaatggcg caaagcctcg gggaaagtcc
    24241 gttgcatatg cacatggtca ttgtctctta tgtactgacc gtggcggtga tgctgcccgc
    24301 cagcggctgg ctggcggaca aagtcggcgt gcgcaatatt ttctttaccg ccatcgtgct
    24361 gtttactctc ggttcactgt tttgcgcgct ttccggcacg ctgaacgaac tgttgctggc
    24421 acgcgcgtta cagggcgttg gcggcgcgat gatggtgccg gtcggcaggt tgacggtgat
    24481 gaaaatcgta ccgcgcgagc aatatatggc ggcgatgacc tttgtcacgc tgcccggtca
    24541 gatcgggccg ctgcttggcc cggcgctcgg cggtctgctg gtggagtacg catcgtggca
    24601 ctggatcttt ttgatcaaca ttccggtggg gattattggc gcgatcacca cactgatgtt
    24661 aatgccgaac tacaccatgc agacgcggtg ctttgatctc tccggctttt tattgctggc
    24721 ggttggcatg gcggtattaa ccctggcgct ggacggcagt aaaggtacag gtttatcgcc
    24781 gctggcgatt gctggcctgg tcgcagttgg cgtggtggca ctggtgcttt atctgctgca
    24841 cgccagaaat aacaactgcg ccctgttcag tctgaaactg ttccgtactc gtaccttttc
    24901 gctgggtctg gcggggagtt ttgccggacg tatcggtagc ggcatgttgc cctttatgac
    24961 gccggttttc ctacaaattg gccttggctt ctcaccgttt catgccggac tgatgatgat
    25021 cccgatggtg ctcggcagca tggggatgaa gcgaattgtg gtacaggtag tgaatcgctt
    25081 tggttatcgt cgggtactgg tggcgaccac gctgggtctg tcgctggtca ccctgttgtt
    25141 tatgactact gctctgctgg gctggtacta cgttttgccg ttcgtcctgt ttttacaagg
    25201 gatggtcaac tcgacccgtt tctcctccat gaacaccctg acgctgaaag atctcccgga
    25261 caatctggcg agcagcggca acagtctgct gtcgatgatt atgcaattgt cgatgagtat
    25321 cggcgtcact atcgccgggc tgttgctggg gctttttggt tcacagcatg tcagcgtcga
    25381 cagcggcacc acacaaaccg tctttatgta cacctggctt agcatggcgt ttatcatcgc
    25441 ccttccggcg tttgtctttg ccagagtgcc gagcgatacg catcaaaatg tagctatttc
    25501 gcggcgaaaa aggagcgcgc aatgaagttc tggcgacccg gtattaccgg caaactgttt
    25561 ctggcgattt tcgccacctg cattgtctta ttgatcacga tgcactgggc ggtacgtatc
    25621 agttttgagc gcggctttat cgattacatc aagcatggta atgaacaacg gctgcaaatg
    25681 ctcggcgatg cgcttggtga gcagtacgcc cagcacggga actggcgctt cctgcgtaat
    25741 aacgatcgct ttgtatttca gatcctacgt tcactggagc atgataacaa cgaagataag
    25801 cccggccccg gtatgccacc acacggctgg cgcacgcaat tttgggtggt tgatcaaaat
    25861 aataaagtgc tggttggccc gcgagcaccg gttccacccg acggcacacg gcggcccatt
    25921 atggtcaatg gtgcggaagt tggcgcggtg atcgcctccc ctgttgaacg actgacccgt
    25981 aatactgata tcaattttga cagacaacag cggcaaacca gttggctgat tgttgcttta
    26041 tctaccttat tagcggcgct ggcgacattc ccactggcgc gcggtttgct ggctccggta
    26101 aaacgactgg tggatggcac gcacaaactg gcagcgggtg atttcactac tcgcgtagcc
    26161 cccaccagcg aagatgagtt gggcagactg gcagaggatt tcaatcaact cgccagcaca
    26221 ctggagaaaa accagcagat gcgccgcgat tttatggccg atatttctca tgaactgcgc
    26281 acgcctttag cagtactgcg cggcgaactg gaagccattc aggatggtgt gcgtaaattc
    26341 acgccggaga cgctggcttc tttacaggcg gaggtcggta cactgaccaa actggttgac
    26401 gatcttcatc agttgtcgat gtctgatgaa ggcgctctcg cctatcaaaa agcaccggta
    26461 gatttgatcc cactgctgga agtggcgggc ggcgcatttc gcgaacgatt tgccagccgt
    26521 ggcctgaaac tgcaattttc cctgccagac agtattaccg tatttggcga tcgcgatcgt
    26581 ttaatgcagt tgttcaataa cttactggaa aacagcctgc gctacactga cagcggcggc
    26641 agcctgaaaa tctctgccga gcagcacgac aaaacggtgc gcctgacctt tgccgacagc
    26701 gcgccgggcg tcagtgacga tcagctacaa aaattgtttg aacgttttta tcgcaccgaa
    26761 ggttcccgca accgtgccag cggcggttcc gggctgggac tcgcgatttg cctgaacatt
    26821 gttgaagcac ataatggccg cattattgct gcccattcgc cttttggcgg ggtaagcatt
    26881 acagtagagt taccgctgga acgggattta cagagagaag tatgaccgag ttaccaatcg
    26941 acgaaaacac accgcgtatt ttgatcgtgg aagatgaacc gaagctggga cagttgctca
    27001 ttgattatct gcgtgccgcg agctatgcgc cgacgcttat cagccacggc gatcaggtac
    27061 tgccgtatgt gcgccagaca ccaccggatc tgatcctgtt agatctgatg ctccctggca
    27121 ccgatggcct gacgctgtgc cgggaaattc gtcgtttttc tgacattccg atcgtgatgg
    27181 tgacggcaaa aatcgaagag atcgaccgcc tgctggggct ggagattggc gccgatgact
    27241 acatctgcaa gccgtacagc ccacgggaag tggtagcgcg tgtcaaaacc attttgcgcc
    27301 gttgtaaacc gcaacgcgaa cttcagcaac aggatgccga aagcccgttg atgatcgacg
    27361 aaggtcgttt tcaggcttca tggcgcggta aaatgcttga cctgacgcct gcggaatttc
    27421 gtctgttgaa aacgctctct catgaaccag gaaaagtgtt ctcccgcgag caattgctca
    27481 atcatcttta tgacgactac cgcgtagtaa ctgaccgcac catcgacagc cacattaaaa
    27541 acctgcgtcg caagctggaa gctctggacg aagagcagtc ttttatccgc gccgtctacg
    27601 gtgtcggtta tcgctgggaa gccgacgctt gccgcatcgt ttagttttag cgacatgact
    27661 ttgttagccg gagatggcgt tcagatcaga tccggcaaca ttatcccacg tatggtcagc
    27721 aaactgacat tatctctccg ccctggcagg caccagaaaa tcgctcatac tttaatcggt
    27781 aaacagcacc tttagatgct gttttcgata cgcaatttca atcaaggagt cattatggct
    27841 ggttggtttg aactcagtaa gagcagtgat aatcaatttc ggtttgtgct aaaagcgggc
    27901 aatggtgaga ctatcctcac cagcgagctg tatacctcaa aagcctctgc ggaaaagggc
    27961 atcgcgtcgg tgcgtagcaa cagcccgcaa gaagaacgct atgagaaaaa aacggcaagc
    28021 aacggcaaat tctatttcaa tctgaaagcc gccaatcatc aaattatcgg ctccagccag
    28081 atgtacgcca ccgcgcaatc tcgtgaaacc ggaattgcct ccgttaaagc caatggcaca
    28141 agccagacgg tgaaagacaa tacgtaatta cgatgccggg cgcgtgttgt tggcgtccgg
    28201 cgctttacca gctcccccct acagcgctac aatgcccgcc cttaaagtgg gggcactccc
    28261 ctggccgctt catcaggtga agcggatctg acctgtcatc agaacgagag aattatgttt
    28321 aaaccggaac tcctttcccc ggcgggaacg ctgaaaaata tgcgttacgc tttcgcttat
    28381 ggcgcagatg ctgtttatgc gggccagccg cgttactccc tgcgtgtgcg caacaacgaa
    28441 ttcaaccacg aaaatcttca gctcggcatc aatgaagccc acgcgctggg gaaaaagttt
    28501 tatgtcgtgg tcaacattgc accgcacaac gccaagctga aaacctttat ccgtgacctg
    28561 aaaccggtgg tggaaatggg gccggatgcg ctgattatgt ccgatccagg gctgattatg
    28621 ctggtgcgtg agcacttccc tgaaatgccg atccatctct cggtacaggc taacgccgtg
    28681 aactgggcga cggtgaaatt ctggcagcaa atgggcctga cccgcgtgat cctctctcgc
    28741 gagctgtcgc tggaagagat tgaagagatc cgcaatcagg tgccggatat ggagatcgag
    28801 atcttcgttc acggtgcgct gtgcatggcc tactccggtc gctgcctgct ctctggctat
    28861 atcaacaagc gcgacccgaa ccagggtacc tgcaccaacg cctgccgctg ggagtacaac
    28921 gtccaggaag ggaaagaaga tgacgttggc aacatcgtac acaagtacga gccaattccg
    28981 gtgcaaaatg ttgagccgac gctgggtatc ggcgcgccaa ccgacaaagt gtttatgatc
    29041 gaagaagccc agcgtccggg cgagtatatg accacgtttg aagatgagca cggcacttac
    29101 atcatgaact cgaaagatct gcgcgccatc gcccatgttg aacgcctgac caaaatgggc
    29161 gtgcattcgc tgaaaatcga aggccgtacc aaatctttct actattgcgc acgcaccgcg
    29221 caggtttacc gtaaagctat cgatgacgcc gctgcgggca aaccgttcga taccagcctg
    29281 ctggaaacgc tggaaggtct ggcgcatcgt ggctataccg aaggtttcct gcgtcgtcat
    29341 acccacgacg attatcagaa ctacgaatac ggttattcgg tttctgaccg ccagcagttt
    29401 gttggtgagt ttaccggtga gcgcaagggg gagctcgcgg cggtagcggt gaaaaataaa
    29461 ttctccgttg gcgacagcct tgagctaatg acgccgcaag gcaacattaa ctttaccctt
    29521 gagcatatgg aaaacgccaa aggtgaagca atgccggtcg caccaggcga tggttatact
    29581 gtgtggctcc cggtgccgca ggatctcgaa ctgaattacg cgctgctgat gcgtaatttc
    29641 tccggtgaaa ccacgcgtaa cccccacggt aagtgattaa tttcgattat ttttcccgga
    29701 tgaaaaattc ttagaaaccg atcacataca gctgcattta ttaaggttat catccgtttc
    29761 gctgaaaaac ataacccata aaatgctagc tgtaccagga accacctcct tagcctgtgt
    29821 aatctccctt acacgggctt attttttacg cgtaatacaa tgaaataaaa ggatttattt
    29881 ctggtcacgt ccacacattg accacatcga caaaaaagcc cctcgactga ggggcttcct
    29941 gtttgtaatt acatccacat aatttgctgc cctgacggca acgggtgcgg tcttacggcg
    30001 tggacttctc ccggcttcac gatgtatcgc tgtaccgact cataagtgat gaacgtggcg
    30061 ctgcaattca cgttctggca ctggtgataa cgctcttttg tcgtgtcagt gatatagcga
    30121 cttgtacgcg catgtgcggc atgctggcat aaaggacaat gaaacatcgc gagcacctct
    30181 tccggttttg ttgatggtgc cattttagtt aatttatcct tataaaacaa acagataaaa
    30241 caaaaacatc actcatcatc ttctgtttcg tactccacat cagaaagcct gacctcaagc
    30301 tccaaggacg tcgtgaagcc gctattattc agaaaatgtg tcaccttagt gattgtccag
    30361 tcctgctcgt ctatgacgcg cttaaagcct gacactttga ccggcgtttc cgtgtaaata
    30421 tctgcccgac cagtagccag actgatggag aactccgcaa cgccccgttg cagcttatcc
    30481 cacttcgcct gagcggcgcg catggcctgt gctttcgtgg catataccgt ggtcagggca
    30541 aaaacgttgt cagcctcacc agccatgtat tcaccttcgc gcgcttccgg tacttttggc
    30601 gctttcttct gcgtgactgg tttcgctttc gggtgctcca gtgcgcgcag gtgtttctct
    30661 ttctttttgc gtttcagttt taccttctgc ttttgtggct tcgggtcttt ggtgtgtaac
    30721 cactttgccg ttacgccggt ataggctcca cggtcagcaa tcgcaaaatg atggcggtcg
    30781 ccgtcgctgc gggttatggt gacctgcggg atttttttac cgctggccgt caccccctgc
    30841 cccgctttga gaaacaacag ttttcccatt tttaccgaca cctcaccgcc gttgcgttct
    30901 gcaagacggg tcaggaattt cgcatcagac tcctgcgact ggtcgatgtg cgggatttta
    30961 attccggcca gtgacggagc gacactggct tccagcctgt tacgggaggc tatcgcctca
    31021 acaatcgcac cgagcgtggt gtcatgccag gagccttcac ggcgggaatt gagcgtcccg
    31081 cgaaaatctg cactccgggc gcggatggtg accacatccg gtgcgccccg gtgttcaacc
    31141 tcatcaacgg taaatttccc tttgcatacc agggcaaaac ctttccagcc gatatacacc
    31201 gtcaggacag cgccacgaac cggcagcccg acctgcccgt cggcatcgtt cagttcaata
    31261 tcaagctggt cagcctcaaa gccccggtta tccgtcaggg tcatgctcat cagtcggtcg
    31321 ctgatattgc cggaaatatc cctgctgtcg agcatcagca tgtaatccgg cgtcagcgta
    31381 ctgcctgcat caaatgtcag tgcatccagc attatcccgc ccccgtcata cccgtgaatc
    31441 tggtcgccat actgccagcc ttaccgatga gcgattccgc ctgtttaccg atatcgccat
    31501 aaagcgcggc cagtgattca tcaacgcggg tgagcgacag cgtaaaatca atttttcggg
    31561 gtgtgccgtc tgcaaagaaa atacttcctg tttcactcac cctgctgatg acatacatgc
    31621 cgtaaatcat gccggtgcca tccagcaacg gccacgcccg gccttcctct gccatcagcc
    31681 tgagcgtggt catcgtcagc ttgccgccgg tcagttctgg ataaagcaca ccggcaagcg
    31741 tgatgttttc ctcacccaca ccgagaaact ggtaggcgtc ccgtttaccg atacgggaat
    31801 ttgacggcca gcgataatct gattcacgct gcatggtctg gtgtggcagc gtctggcgca
    31861 taaaaacaaa catacctaac gcgagcatca tttttcgtca cctccttaac cgtcatgcat
    31921 catgctggca cgggcgcgcg cacgtttatc ccgctcgtat ttttcgagcg catcctgtaa
    31981 ctggcggtca agctgtgtcc ccggcgcagt accacccgtc aggctgatgt gatattcgtt
    32041 tttactctgg tccacataag agcggccagc cggtgccgtg accggctgat aagcctgata
    32101 gcctgcataa gagctggtcg ccggaatata accaccggtg ccatacgtgg cggcatgagt
    32161 tctggcggcg gtctggtcaa gtgtgtctga ctctttgttg ataacaccga gtttttccag
    32221 tacccagtca ataccgctgc gcagtttgtt gaacgcatta agcggcagca tcagcgcgtc
    32281 agccagtgcc tgcccgaaca tgacgcccgt gtcacggcaa cggttcaggg tgtcctgtgt
    32341 ggctttaacc ggagcaatca ggtttgtaaa ccactgccac gcggcctgta acttttcgcc
    32401 cagccagtca aacaccggct taagtggcgt gaacagctcc cccaccggcg caaatgccgc
    32461 tttcagccct tcaaccacac cgccaaagaa tgcgctgaca ggctcccagt atttacggat
    32521 aagcaacgcc ccagcgacaa tggcagccac cacggccaca accggccagc taatcgcacc
    32581 gatggccgtc ataacggcac taccaaccgt cgtgaagatt gcccccattg cgcctgctgc
    32641 cgcgataatg gcattgatgc cggtgataac cggccaggct acaaggccaa tggcaccgat
    32701 gatgccagtc agcaccagtg caccgccggc aatgatgccg atggttgacg ccagtgattt
    32761 gtttttctgt atccagccgt cgagttttaa cacatacttt gtggccgtct gcgtgagctt
    32821 acgcagtgcg ccttcctgct ggtcaaacag gtcagtcccc accgcctcat aagcggactg
    32881 aaactcctta aagtcaccgc cgagattgtc ctgcataata tttaccagct ctgaggtctt
    32941 cccgtccgag gctttaaacg cagcggtcag tttgtccagc tttccggttg aggcgacagt
    33001 catcagcacg gcggcggctg agctggcctc ctccccgaaa atggttttca tgtattcagc
    33061 ctgctgggca gtaccgagcc ggtttttctc aaaactggcc tgcatttctt tcagaatggt
    33121 aaatactggt cgggtgtttc ccttgctgtc tgaggttttc actccaagct ctttgagtgc
    33181 atcccatgct tttcccgtcg gtgcctgcag gcggcttaac acggcacggc ttcccgtccc
    33241 cgccattgag cctgtgattt ttgcatcatg cagcgccccg accattgcgg cggtttcttc
    33301 aatgctgaca ccggcatttt ttgccacagg tgcggcatag gtcagcgcat cgctcatgcc
    33361 gtcaaaatcg gcggcggttt tgttcatcgt catggagaga acatccccga tatgagcgac
    33421 cttatcgttt gaaagctgaa aggcggattt catccccatc agcagggcgg cgttttcttc
    33481 catcgtgcgg cggttcgcca gcgccatatt cagcgtgacc ggcgttgccg cctgaatggc
    33541 atcaacatcc ccacccgctt tcgcgatgat tatctgtgca ccggccgcat catccgccga
    33601 ggcggcggta ttgtcgccga gctggcgcgc ctgtttgcgt agtgcggtca tttcggcgga
    33661 gtcttttgcc acacccagca cggcctgcaa ttctgagttt ttctgcgcaa actcataacc
    33721 ggacatcagc aacttaactc cggccatcgt ttccgccgcc gcaatcccca caccggcagc
    33781 gcccactgag gccatatttc cggccagttc ctttccggcc tgataacgct gttttactgc
    33841 attaagtttt gcctgttgcg cactgacacg cgccagcgcg tcacgctgcc ggttaagctg
    33901 tgcggtggtt tcactgatac ggtttttcag tccctgctca tcatgtgcaa gattgcgggt
    33961 attaattccc acagcggcca gttcccgctg ctggcgctta acggaatccg tcaggcggtt
    34021 atatttcgcc tgtaagtcct ccgccgcacg ctttgcggat tccagcactt tcgcctgagc
    34081 acgggtcgga cgttcagtgt ttttaaactg tgtggcaagt gcttcggctt cctgccgtgc
    34141 cttttcaagt gcatgaccag tcacggcaag ctgtgcgctg gtcttgcgaa atccctcaat
    34201 acgggatgcg tgaccgttca gctcgcgcag tgatttttgt gtttcccgga tatcccccga
    34261 cagcgactta ctcgctgtac ggatggattt aaacgggcgg gatgcctggt caacagccct
    34321 gagcaatacc tgtaatttta cattgttact cattcgtgct tccgcttcgc cggagcgcct
    34381 tttcgcgcca tgtgatgagt tcggtcaggc tcatgggata cagttctgat ggcggccagt
    34441 gaaatatcac tgccacatct gccatcaggt catcgaccga cagatttttc ggaaacgtca
    34501 ctgcaccgag ttcggagaca aaaaaccgac caccttaccg gccagcgcca caaggtcagg
    34561 cagttccagc gcggcgacct cctgctcggt cagcatcggt gccgtcatgc gcggcagcac
    34621 cttaatcagt gcatcgactt cggagtttgc gaccgcagcc agactgacac cgcgcagcgt
    34681 cccggcatta ggtttcatca gcgtgacctg ttcgataacc tgctcaccac gcttgaccgg
    34741 attgtccagg gtaatcacat tttctttgtt catggttttc tcacttctga atcggggtta
    34801 accggtcagc caggctgacc ggatgaaaat cacaggccga tattgcggcg gtgttgctcc
    34861 agccggtcga cgccgttcac cttctcaatc atgttgatgg tgtcgatttc gaccagctcc
    34921 ttaccgtcca tcgtcaactt gaaataagta caaatcaccg ggagtttcga ttcggtatcc
    34981 tctccctgtt taccctctcc ggtatcgact tctttctgac gcccacgcat gaccacttcg
    35041 acggccaccg tttcgccggt atcgtcgcgc tggtaagaac cagcaaaacg aatcggtacg
    35101 gcatcagcac tggttgcagc gtaaagctcc cagataaccg aatccgggaa tccaccgagc
    35161 gaccactcca ttgacagagc atcatcatca aggccgaggt ctaccggtgc gctgccgttc
    35221 atccccgcac cgcgatagtt ttcgagctta cgggtcagtt ttggcagcgt gacggacttt
    35281 gcaacgccct gatagctgta gccgttcaga aagacgttca ttaacttgag tttgcgcggc
    35341 attgccatcg gtcaggctcc ttaattgctg ttaaccgaag tgaccagact tgccaggtat
    35401 ctggaagtaa tacgctggcg cagggtcagg ttttcgagag gaggcaccgg ggtatagtcg
    35461 tagtcgatat acagttttcc ggccttgagg gtttccgcat cgttggattc ttcgctgaac
    35521 cagcatgtgc catccacgat ataaccgttg tttttcagtt cacggaattt ggcattgatg
    35581 ccgtcaatga tgtcgcgaat cagcgttgcg gtgatgggct tatcaatagc ccacatgtgc
    35641 gcctcagcca tcgtgtcggc cagtacctgc gcggtgcgtg tgtagctttc aaagaggaac
    35701 agcgggtcat cagagcaggt acggttaccc cagaaacgga aaccgtcgcg gcgaaccagc
    35761 gttgtgacgc ctgcctcgtt cagcaggtca gcatcagtgc ctggcttctg caaatcccag
    35821 aagacggatg cactgatgcc ggtaacaccg ttcaccccga cgttggacag cgttttatgc
    35881 cagccctgct cctggtcgat tttagcgcgc agacccagcg cacgagcggt ggcatacgcg
    35941 gtggcggtgg tactggtaac cgtatcccat gcgaggaaat ccggccagat aaccatcagc
    36001 tcacgctggc tgaaattctg gcggtaggct ttcacctcgg aaatggtttt acaaccccat
    36061 gcgctgatat acccgaaggc attcagttcc tggcataccg atgccagtgc gacggcgacc
    36121 tctttggtgt ccagtcccgg tacgccgaga atgcgcggtt taacgccggt aaccgactca
    36181 gcccccatca gggctttcag tccggtgtac tgaccgtttt cgtcggtggt gccgataata
    36241 ttggaaacag tctgcgcgag tttcgtttcc tcgtcgtcgc cggtgccgtc ttccacgcgc
    36301 acaacaacgg tgaccggttt tgactggtca gcgatggcct gtaacgacgc cgccagcgtg
    36361 ccttttttac cggcctttgc aattgcgctc tgcacattgg taatcagcac aggtttattg
    36421 agggggaatg tttccgcatc cgcatcgctg gccgtgcaga ccatgccgac aatggcagtg
    36481 gatacggtgg aaatgacgcg ggtgccgtcg ttaatctcca gcacctgcac gccgtgatga
    36541 tagtcactca tccgtctaac tccgtggtta atgggtgagt ggtattttct gttgtgcaga
    36601 gcatgagacg ctatttgacc tggctggtca gtggatgaaa caacagataa agaaaaggcg
    36661 gacaatccgc ccgcctgtca tgatttgtac tcacttattt cccgactgac aatttacata
    36721 gccaaaacgc tatcaaatct gacagtctgc tttgagcgag aagcggaagt tcaatgtgat
    36781 aaagctcagg aacattgtcg ttgatgatga taaagaggat cagactaact tagcacttct
    36841 catcacgttg ataaggcata gaaactgcaa acactattgt gaatataatt tcactatagt
    36901 aaattaaagt aagctctttt cgttcgttat tacataaaat aaagttctct ctaaggttga
    36961 aaaatgttag atatcagaat gtcacgacca tctgaagcat cagagatcat acaaatctgg
    37021 aagagttcag tggatgctac ccacgacttt cttacaggtc atgaccgaca agaaatcgaa
    37081 aaagaagtta tcggcttttt ctcagaaact cctgtatggg ttgcaacaaa tcaggatgac
    37141 caaccgctag gatttatgtt tttgcatgag gggcatctgg aggcactttt tgtagcaggc
    37201 tctgctcgcg gacttggcgt cggaaaacgt ttgatatctc atgcactggc gctgcatcca
    37261 gagctaagcg ttgatgtaaa tgaacaaaac cagcaggctg tagggtttta tcagcatatg
    37321 ggttttcagg tatcagggcg ttccgatctg gataatcagg gaaggccata ccccttgttg
    37381 catctgagaa tggccaataa catgtaacca atgtggcaga gaagatatca ttgatttttt
    37441 tggctaaaag ccacgatgta agaacagctc tgctcactat ctggattgct catttggtac
    37501 tcgcttgcaa aggtaagggc aagacagtca ccggcacgta aatgatgggt ctgatttttg
    37561 actcgaaaat caaggctgcc actcatcatc caaagcgttt gcccggaaag atgctcattt
    37621 gcggaggcgg gtatcgtcag ctcacccatt gcagggattt caactttgat cagttccggg
    37681 catgcccccg caggagacaa agaccaacgg gtgatgcctg attgctcatc cgtccagtgc
    37741 tgttgctgat gagcaaaacg aaccagggaa ttctgatgtt gctcaagctc tgcaaagagt
    37801 ttagatagcg ttacattcat ggcgttggcc aggcggctaa ggatagttgc actagggctg
    37861 gagatgcctc gttcaatctt actaatcatg gcctgactga cgcccgatcg ttgagcaagc
    37921 tctgttacag tcaaattgcg agccttcctg tgtttaagca ataaacgtgc aatatcgcta
    37981 tctactatct gatttttaaa ttttttatcc atttccgccc tctctttcac caacctgatt
    38041 atgccattac ataaattggc ctgaaataaa tgattttgta agtctgtaaa ttcactactc
    38101 ctaagcgcgg cacttcgttt atacgcacct ataaccttaa aatatgagtt ggtaagctta
    38161 acgtccgctt ttggcacata gcggactgtc agattaggct ttactctgtg ctatagatat
    38221 gtaagctcac accagagatc atacaactta ttgcggcatt tccggccatt cgggatttgc
    38281 aggatccaca cgactgacca gaacgctgta gcgttcccat gcttccagtc ggctgcgttc
    38341 ctcatccgtc gccatattca gcctgacagc gcgttccagc ggcaaaatca cggattcagc
    38401 ttcggaaagt aaagctgcct tttgtgattc tgcctgttgc tcctgttcat ctgccgtata
    38461 aatccgctta atcacggcac catccttaaa catccattta cctgagtcat cagcacgtcg
    38521 gttggaggta atatcaggaa cctcgacaac gctgaaacct tcagggttaa gcgttgaagc
    38581 atctctggtg atgccgacaa ttatattatt ctcgtcgtaa acaatcttta tcgtgtcttc
    38641 ctgaaaatta cttacttcct cataccagtt ttttccctct tcggaccata accagataac
    38701 atcaaaattt tttgtcagtt gatattgggc aacagttttt ggattacccg cagtaatatt
    38761 ttttaaatgc tgcataaatt acacctgtgc gacgttatac catgtgccat tgatgtattt
    38821 ttgtattggc ctgaatactg cggggtcatc accatcgact tcaccgacaa taccaagccc
    38881 ggtaattacg tgccctgctt tctcatacat cacccctttc tgcatggtct ggacaacacg
    38941 tgtgccaagt ctgatatctc tcacatagcg ggaatcaaag ttaccgtaat ccgagggatt
    39001 aacacgcccc gtaatattta tggtcttatt actttgaatg cttccggaaa caaagcgcat
    39061 aacatggacg ttattagcat aaacatccag attaccatcg ccattttgtt taaatccggt
    39121 gtcgttatca ccaagaacaa tagagttccc acccaacgcg ttattcgtgc caagtgccag
    39181 cccgccatca atcctggcac cattaccaac agacacaact cctgttctta agttgatgcc
    39241 gaatggcctt aatgacccaa tatctccatt ttcaccctca ttttctcgtg taggaatgat
    39301 atacaggttt tcttcagaac ggcgaaaaat agcaccaaaa gatgaattaa atatcctcag
    39361 cgcattgact gtagatattt ttacttcact actgaaaagg gctttaacaa gaacatacag
    39421 agcatcccat ttaagattca tcaggtcttt tgttgtggtg ctctggcgac ttctccattt
    39481 gaaatattca ttgccgttgt cacctgtttc aaaccacata tatgaatcag tatcgctgtc
    39541 ggcatcattt ttaaatccaa tctttgccca gtcagtattt cgaatccagg caaggattga
    39601 gtcgttttca aaagtaagcc cgccggacaa ggtatcgcca tttttttgca cggcgttccc
    39661 ggctcggttt accgtttcct gtaaaccgag atattcgata acggcggcaa cggtcgattt
    39721 agccagaata tcccgcccga cttttgtcag ggtcgccagg ctggcaacat cattccccgt
    39781 aaaatacgga aacctgtctg ccgcagtagc aagccccgcc agcgccgtca aagtggcatc
    39841 tttcggttgc ttacccgcaa gcgcattagt catggtggta gcaaaattcg ggtcattgcc
    39901 cagcgccgcc gccagctcgt tcagcgtatt cagtgcgtca ggcgacgagt ctacaagggc
    39961 ggcaatcgcg gccataacga aagccgtgtt tgcgatctga gtattattcg ttccctgtcg
    40021 cgcagttggc gtcgttggcg ttccggtcag tgcagggctg tttaatggcg ctttcttgtt
    40081 cgtttcatcc attaccgtct taacggcttt tggtgttgcg gcgagcgttt cagacatgct
    40141 gttggtcgca ctacttagct ggacaagacc ttttcgcgct gtggtagcgt cctgtgcggt
    40201 atatttcccg ttagcaaggt catacgcggc ctttaccgct ttcggcgttg cggccagtgt
    40261 ttcagacgtg ctgttggtcg cactgcttaa ctgagtaaaa cctttggcgg tcagcgaggc
    40321 gtccgggtga cgtcgtgact gttcgtgctc tgcaattttg tcatcaacgt aatcctgtgt
    40381 cgccatcacc gttgtggtgt caatggtcag ctccaccgag gccacactgc tgacgatgat
    40441 gaccatgcga caggtctgcg aacgccctga gccttcggca agagctggct tataactttc
    40501 ggccatgttc gccacggcaa ttaacgttcc cgcatcatcg tacaggccaa gctcacgcat
    40561 ccagaaaccg cccacctccg gcggaataac cagctctgcg ataatataat tactgtttcg
    40621 tttgtcctgg ctgattttgt tcagcgtatg tcgccagact tcgtggataa gcccggtctg
    40681 tccggcatcc gggacaggca atttaccacc gccatccccg acggccatcg tggtaatgtt
    40741 gaccttccgc cctcccggtg cggttgccgc tgccagcttt gctgcaccgg cagtggtgat
    40801 aacggttttg aattttgtgc tcattattcc tcacttatcc ggggtaaacc gtaattacat
    40861 cgccgtcgta agccacacca ccggcgaaca ggtagccggg aatgtcccgg gtaatgttca
    40921 ggccaataag gtggcggctt gcaggtttgg catcagcaat cagccgttcc atttcctgat
    40981 acattgcctc tgtgatgcca ctttccagta caccaatatc aagccggaag gtgcccggcg
    41041 ggtcactggt ttcccaccac tccgtcacgt tgatgagata gcccagcggc tccaccacac
    41101 gccggattgc acctatagtg cctttatgac agtggatgaa ataggcatcg cggataacag
    41161 cgcgttttgt cgcttccggc cacttttcat cccatctgtc gaccgaaaac gcccacgcca
    41221 gccacggcag cagatttgcc ggacaggagt ccgggttcca cagctcacga atactgaccg
    41281 gtgttttttc aatttccgca caggcttttg cggcggcgac ctcaagcggt gatgagccgg
    41341 tcggcaacag tcgcgaatca ctcatccgag cctccggtca cgacgcggta ttcggtacag
    41401 aaagacgcct gcgtactgtt gagcacgatg tcggccagtg gtgcggccag ctcgacacgc
    41461 tgcacgcctt ccacatgcaa agcggcataa atggcagaca gacggatgtc gcgccccagc
    41521 cggtgctgtg ccgtgatgta cgcttccagt tttttcacgg cggcagcgcg tatgggttcg
    41581 ctttcgggac cagggtaaag gtaaagcgtg gcgtttatct ggtattcaac gatggcggca
    41641 gactgcacgg tcacgcggtc ggccaccggc ctgacgtcct cgccattaag ggcgttacgc
    41701 accaccgcca gcaggtcttc ggatgcgaca ccgttatttt cacgtgacag cacagagatg
    41761 gtgacgcagg ccggagacgg actggtgaca gagatatccg cgacacgccc gtcagcactg
    41821 cgaccatgat actgataggc acccaccgac ccggcgacgc ttaaaccttc aaaggcctgc
    41881 tgaatacgca gacgataatc ggtgtcagat tccatcacgg ccggtgtcgg cgggatggtc
    41941 gaatcatctg ccggggtgat agtcagacgc gtggtgttgt aattggcacc aatcacatca
    42001 aggtcattac ccgcggcaca ggccagcatc accgcccgtg cggcctcatt cacacgctga
    42061 cgccagagaa gctcacgata agcattttcc tccagcagtt tgacgagagg ctcagattcc
    42121 agcgtcaggg tacgggcgac cgcctcctgc tggtcttccg ggtaaaggga aatcagtgtc
    42181 gccttgcgtt cggcgagaat ggtttcaaag tccagctcct cgaccacatc cggtgcgggt
    42241 agctggttca ggtcgataat cggcatggtt tcaactcaca gggatggtta acgaaagtgg
    42301 ctggccggtg tcgttgtgct ggccggttaa cgtgaccgtc attcgcccgt caaaactgcg
    42361 cgccgtggtg acagatgaca gcgtgacgcg gggttcccat ttcagcaccg ccatgtaaca
    42421 ggcgacctta atctgcaact caagcgccgg ggtctgcggc tggtcaatca ttgatgccag
    42481 caacgagccg taatcacgac gcatcacccg tgagccgacc ggtgtacgca ggatatcgcc
    42541 gatactctgg ctgatatgct caaggtcagt gacagtcagg ccattactgc gattcattcc
    42601 gagataacga gctgtcataa aggactcccg gttgtgccgc cgctgtcgcc ggggtgtttg
    42661 tgggtatgca gtaccttacc gtttgatgag agttcaccgc cggtgtgttc aatgttgcca
    42721 cgcatcgtcc cgcccttctg tacttccagc gtgctagtaa tcagcctgtt ggtgcagacc
    42781 acctccggtg tgtccagggt gatgcgagtt gacgctttca ccatgaccac cggcaccgtg
    42841 gcagtaacag aatcagaagc cgtcacgctg gccgttttaa ttccgcttac cgtgagtgca
    42901 ctggtttcgg gttcatactc aatcaccgcc ccgtcaggga aacggatatg cagggcatcc
    42961 gccgacgcag acggcgcggg gttatcgccg gaataaatcc ccggcagaac gaacgccgtg
    43021 tcaagttcac cacccacggc cagaatcagc acctgttccc ccacggaagg tgcccaccat
    43081 gtgcgcgaac gcccggcgcg atgggtcagc cactgaagcc agtcggtgca catgccgccg
    43141 gtctgcacac ggcagcgacc ggcgttaagg tcggtttcga cgacaaggcc ggtacgaatc
    43201 atgttgcgca gtgcgcgcgc gagttcctga atatttgcga gagtgttcat gcgtgtgaga
    43261 ttgcacaata tataaaagtt atgctatctg gattcatttg tagaacgacc atacaacatt
    43321 cgaggagagc gtaatgttca gtgataatgt gattaatgcg tggtggttta tctctttgta
    43381 tctattttta ttaatagcat taacatttg
//
LOCUS       DAZKGF010000020        39812 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZKGF010000020 DAZKGF010000000
VERSION     DAZKGF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.020"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..613
                     /gene="zapC"
                     /locus_tag="ACHOQV_001059"
     CDS             71..613
                     /gene="zapC"
                     /locus_tag="ACHOQV_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFP9629471.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFNLEQAV"
     gene            complement(610..1719)
                     /gene="ycbX"
                     /locus_tag="ACHOQV_001060"
     CDS             complement(610..1719)
                     /gene="ycbX"
                     /locus_tag="ACHOQV_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFP9629472.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEVLATAPAKIYGAAA
                     ADDTVNITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            1963..4071
                     /gene="rlmKL"
                     /locus_tag="ACHOQV_001061"
     CDS             1963..4071
                     /gene="rlmKL"
                     /locus_tag="ACHOQV_001061"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFP9629473.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPVMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4083..5990
                     /gene="uup"
                     /locus_tag="ACHOQV_001062"
     CDS             4083..5990
                     /gene="uup"
                     /locus_tag="ACHOQV_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9629474.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKIEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6120..7373
                     /gene="pqiA"
                     /locus_tag="ACHOQV_001063"
     CDS             6120..7373
                     /gene="pqiA"
                     /locus_tag="ACHOQV_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFP9629475.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADDPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7378..9018
                     /gene="pqiB"
                     /locus_tag="ACHOQV_001064"
     CDS             7378..9018
                     /gene="pqiB"
                     /locus_tag="ACHOQV_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFP9629476.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9015..9578
                     /gene="pqiC"
                     /locus_tag="ACHOQV_001065"
     CDS             9015..9578
                     /gene="pqiC"
                     /locus_tag="ACHOQV_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFP9629477.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            9834..10001
                     /gene="rmf"
                     /locus_tag="ACHOQV_001066"
     CDS             9834..10001
                     /gene="rmf"
                     /locus_tag="ACHOQV_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFP9629478.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10071..10589)
                     /gene="fabA"
                     /locus_tag="ACHOQV_001067"
     CDS             complement(10071..10589)
                     /gene="fabA"
                     /locus_tag="ACHOQV_001067"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFP9629479.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10658..12418)
                     /gene="ycbZ"
                     /locus_tag="ACHOQV_001068"
     CDS             complement(10658..12418)
                     /gene="ycbZ"
                     /locus_tag="ACHOQV_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9629480.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTLTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANIR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12604..13056
                     /gene="matP"
                     /locus_tag="ACHOQV_001069"
     CDS             12604..13056
                     /gene="matP"
                     /locus_tag="ACHOQV_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFP9629481.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13132..14184)
                     /gene="ompA"
                     /locus_tag="ACHOQV_001070"
     CDS             complement(13132..14184)
                     /gene="ompA"
                     /locus_tag="ACHOQV_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFP9629482.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVPGGASTKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(14540..15049)
                     /gene="sulA"
                     /locus_tag="ACHOQV_001071"
     CDS             complement(14540..15049)
                     /gene="sulA"
                     /locus_tag="ACHOQV_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFP9629483.1"
                     /translation="MYTSGYAHRASSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15267..15896
                     /gene="sxy"
                     /locus_tag="ACHOQV_001072"
     CDS             15267..15896
                     /gene="sxy"
                     /locus_tag="ACHOQV_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFP9629484.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15859..18021)
                     /gene="yccS"
                     /locus_tag="ACHOQV_001073"
     CDS             complement(15859..18021)
                     /gene="yccS"
                     /locus_tag="ACHOQV_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFP9629485.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18031..18477)
                     /gene="yccF"
                     /locus_tag="ACHOQV_001074"
     CDS             complement(18031..18477)
                     /gene="yccF"
                     /locus_tag="ACHOQV_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP9629486.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18600..20654
                     /gene="helD"
                     /locus_tag="ACHOQV_001075"
     CDS             18600..20654
                     /gene="helD"
                     /locus_tag="ACHOQV_001075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFP9629487.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPGEKVVRLHGTEWGETQRFYHHLDAHWQRWSSEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEVIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPSVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20686..21144)
                     /gene="mgsA"
                     /locus_tag="ACHOQV_001076"
     CDS             complement(20686..21144)
                     /gene="mgsA"
                     /locus_tag="ACHOQV_001076"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP9629488.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21240..21902)
                     /gene="yccT"
                     /locus_tag="ACHOQV_001077"
     CDS             complement(21240..21902)
                     /gene="yccT"
                     /locus_tag="ACHOQV_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFP9629489.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22075..22488
                     /gene="yccU"
                     /locus_tag="ACHOQV_001078"
     CDS             22075..22488
                     /gene="yccU"
                     /locus_tag="ACHOQV_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP9629490.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22533..22850)
                     /gene="hspQ"
                     /locus_tag="ACHOQV_001079"
     CDS             complement(22533..22850)
                     /gene="hspQ"
                     /locus_tag="ACHOQV_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFP9629491.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22908..24098)
                     /gene="rlmI"
                     /locus_tag="ACHOQV_001080"
     CDS             complement(22908..24098)
                     /gene="rlmI"
                     /locus_tag="ACHOQV_001080"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFP9629492.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPNESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVNIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24193..24471
                     /gene="yccX"
                     /locus_tag="ACHOQV_001081"
     CDS             24193..24471
                     /gene="yccX"
                     /locus_tag="ACHOQV_001081"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP9629493.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLAGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIH"
     gene            complement(24468..24797)
                     /gene="tusE"
                     /locus_tag="ACHOQV_001082"
     CDS             complement(24468..24797)
                     /gene="tusE"
                     /locus_tag="ACHOQV_001082"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFP9629494.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24888..25547)
                     /gene="yccA"
                     /locus_tag="ACHOQV_001083"
     CDS             complement(24888..25547)
                     /gene="yccA"
                     /locus_tag="ACHOQV_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFP9629495.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(25957..26976)
                     /locus_tag="ACHOQV_001084"
     CDS             complement(25957..26976)
                     /locus_tag="ACHOQV_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9629496.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRTIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRSA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(26954..27196)
                     /locus_tag="ACHOQV_001085"
     CDS             complement(26954..27196)
                     /locus_tag="ACHOQV_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP9629497.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(27264..29702)
                     /locus_tag="ACHOQV_001086"
     CDS             complement(27264..29702)
                     /locus_tag="ACHOQV_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9629498.1"
                     /translation="MSTDKEEFALYCEAKNDKVRKRFGIKGGFYWSTEKKLSVAISRC
                     VVAMDEAGYDADDFKKPVRVHFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQEKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPDLVHTLDTLD
                     VEAAAATLPMDFNIYEIPVSVYRRAKEIVLKKESPFREWSAALRATPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINANLTETDHENPTAETLMAARHTPEKDILEEINDGLA
                     AECKTEEEKNDEENPQPSGAMADEQATAETMAADTVEHHQDPQPLDDKSQVKVTAEEV
                     NKIMEAADISRPDADKLLAASRGEFVEGISDPNDSKWVKGIETCDSVNQNQLETEQND
                     QKAEQNSQNALQDEPETKQSEPVAQQEEEKVCTACGQSGGGNCPDCGAVMGDATYQET
                     FDEKNQAEVQEDDPEEIEGAEHPNNENAGNDQHHTSDSETGEAADPLIAVNGHHEITS
                     TSRTCDHLMIDLETMGKNPNAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRD
                     TIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILR
                     RSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYV
                     SAIWQKLIPSQADF"
     gene            complement(29795..29983)
                     /locus_tag="ACHOQV_001087"
     CDS             complement(29795..29983)
                     /locus_tag="ACHOQV_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9629499.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
     gene            complement(29980..30168)
                     /gene="dicB"
                     /locus_tag="ACHOQV_001088"
     CDS             complement(29980..30168)
                     /gene="dicB"
                     /locus_tag="ACHOQV_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9629500.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            complement(30654..30806)
                     /locus_tag="ACHOQV_001089"
     CDS             complement(30654..30806)
                     /locus_tag="ACHOQV_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9629501.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
     gene            31081..31383
                     /locus_tag="ACHOQV_001090"
     CDS             31081..31383
                     /locus_tag="ACHOQV_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFP9629502.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNVKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            31386..31745
                     /locus_tag="ACHOQV_001091"
     CDS             31386..31745
                     /locus_tag="ACHOQV_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9629503.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(31792..32184)
                     /locus_tag="ACHOQV_001092"
     CDS             complement(31792..32184)
                     /locus_tag="ACHOQV_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9629504.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            32311..32571
                     /locus_tag="ACHOQV_001093"
     CDS             32311..32571
                     /locus_tag="ACHOQV_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9629505.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            32568..33005
                     /locus_tag="ACHOQV_001094"
     CDS             32568..33005
                     /locus_tag="ACHOQV_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9629506.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            33092..34102
                     /locus_tag="ACHOQV_001095"
     CDS             33092..34102
                     /locus_tag="ACHOQV_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFP9629507.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEVYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSELHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAIQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWR
                     DD"
     gene            34095..34556
                     /locus_tag="ACHOQV_001096"
     CDS             34095..34556
                     /locus_tag="ACHOQV_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9629508.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISGSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            34590..35306
                     /locus_tag="ACHOQV_001097"
     CDS             34590..35306
                     /locus_tag="ACHOQV_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9629509.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLIKAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPVADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEDIKTETVADIVQSLPSFTETRADDLVLPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDILRDITATRELLR"
     gene            35339..35620
                     /locus_tag="ACHOQV_001098"
     CDS             35339..35620
                     /locus_tag="ACHOQV_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9629510.1"
                     /translation="MILRQCAGTMTVESIGSLIGRSGAAVRTKARELGICMMLRGDYH
                     QSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQA"
     gene            35617..35922
                     /locus_tag="ACHOQV_001099"
     CDS             35617..35922
                     /locus_tag="ACHOQV_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP9629511.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHKAEEALKQEGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            35909..36511
                     /locus_tag="ACHOQV_001100"
     CDS             35909..36511
                     /locus_tag="ACHOQV_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9629512.1"
                     /translation="MRKINYQTLREIAKLATQGEWVAFISPGTDTYAVHTPGDERCGD
                     IIKWPGFDDQKNAENNAEFIAAFNPEVVQALLDEREAQSKRIAELEANLVALAAENAL
                     ARKAVQAFCDVVGDNTEVICEEVGRDGVLVILEAMKATGNMPATDAFLADVRAEVFND
                     LCAAFVRHAKVAGLDDADTVTFKEATEALLHCAEQLRAPE"
     gene            36607..36789
                     /locus_tag="ACHOQV_001101"
     CDS             36607..36789
                     /locus_tag="ACHOQV_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629513.1"
                     /translation="MRKFKIIIETGIAGGDFEDVFEVDDDATPDEIHDEAKEIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            36782..36967
                     /locus_tag="ACHOQV_001102"
     CDS             36782..36967
                     /locus_tag="ACHOQV_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629514.1"
                     /translation="MADFGSTKYNASFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGEDDED"
     gene            37073..37276
                     /locus_tag="ACHOQV_001103"
     CDS             37073..37276
                     /locus_tag="ACHOQV_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629515.1"
                     /translation="MTIVKTHTGIVITKDGPQVKKLHQTERMWVVDKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPVEVKHV"
     gene            37269..37508
                     /locus_tag="ACHOQV_001104"
     CDS             37269..37508
                     /locus_tag="ACHOQV_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629516.1"
                     /translation="MFKQNEKSIAQIAEYIPRACRGMQLQEAKARLEKKIALYINDGC
                     DAAVLNAAFAPALNSHTRESFFSCIAAQIHKGGNQ"
     gene            37505..37849
                     /locus_tag="ACHOQV_001105"
     CDS             37505..37849
                     /locus_tag="ACHOQV_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9629517.1"
                     /translation="MSKIDYQALREAAQNYRSMLAWYQEKPDSPNAEQDCDAALSAFK
                     REIRHREVDIIADLLDELEEAKQRIDEQESRTVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            37851..38294
                     /locus_tag="ACHOQV_001106"
     CDS             37851..38294
                     /locus_tag="ACHOQV_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629518.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VVYMWDSERKDIDAPGYYRTEHLVFAESSVKQWGGRVVPLYTAPPAPIVPAVLERLRA
                     IVADPRALPRRKEWVSGQQYSYVLLENVEAMVDEACRAAMLQGDK"
     gene            38294..38437
                     /locus_tag="ACHOQV_001107"
                     /pseudo
     CDS             38294..38437
                     /locus_tag="ACHOQV_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            38465..38995
                     /locus_tag="ACHOQV_001108"
     CDS             38465..38995
                     /locus_tag="ACHOQV_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629519.1"
                     /translation="MEEAKELHEDLVRSHISKALSGEKMKKKDLDADLCWIHGVIVQA
                     SWFVKASLEQNALTGNSQVIPDDGREQFETLIRFHADDKNHETLLLRANEGMNYRDPN
                     VDLAWIFWKSSREHILPGKSDNNPALGDQVSELTMLVKRLASSLKSVNKSSKLPDKAM
                     DYLKRNGLISAEDILR"
     gene            39293..39448
                     /locus_tag="ACHOQV_001109"
     CDS             39293..39448
                     /locus_tag="ACHOQV_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9629520.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(39555..>39812)
                     /locus_tag="ACHOQV_001110"
     CDS             complement(39555..>39812)
                     /locus_tag="ACHOQV_001110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629521.1"
                     /translation="KTNSPPQEIQECILSEWQRTPLLATITSQKIGKYYSVLAVGDNA
                     DIYKLADGSTIIDFYSLRGVLDPTNGKSKRIQGIKSCISTK"
BASE COUNT        10256 a         9386 c        10408 g         9762 t
ORIGIN      
        1 ttgttgaggt tattaagcga agcgacaatg gattgtgttg ctgcggtttt atagggtagg
       61 ggagaggcag atgcgaatta aaccagacga taactggcgt tggtattacg atgaagagca
      121 cgatcgaatg atgctcgatt tagccaatgg tatgctattt cgctcacgtt ttgcgcgcaa
      181 aatgttgact ccagatgcct tttctcccgc aggcttttgc gttgacgacg ccgcgctcta
      241 tttctcattt gaagaaaaat gccgcgattt taatttatct aaagaacaaa aagccgaact
      301 ggtgcttaat gcgctggtag caattcgtta tctcaaaccg caaatgccga aaagctggca
      361 ttttgtttcc catggtgaaa tgtgggtgcc catgccgggc gatgcggcct gcgtttggtt
      421 gagcgacact cacgagcaag tcaatttgct ggtcgttgaa tctggcgaaa atgccgcact
      481 atgcctgctg gcacaaccct gcgttgttat tgcgggtcgc gcgatgcagc tgggcgacgc
      541 catcaaaatc atgaacgaca ggctgaaacc gcaggttaat gttgacagct tcaacctcga
      601 acaggcagtc taacgcgtca gcttaagcgc agtcttcggt acacagctac agcaaagaat
      661 ggtgccatca tcgcccattg ctgatttttt cagcggcgtg acttcgcctt ctaaaagctg
      721 aacacggcaa cttccacaaa tgcccgcgcg gcaagaataa gggatacgaa ttccctgatt
      781 ttctaattgc tccagcaaca cctgctggtt atttccacga aatgcctgtc cctgccagtc
      841 aatatctaca tttgcgtccg gttgttgcgt gatgttgacg gtatcatcag cggcggctgc
      901 gccgtaaatt ttagccggag ccgttgccag aacttccacc tcatcgccaa cgcgaattac
      961 gccgctatta cgggcaatta aattctgacc aaaatcgaca tcgccgttat cctgggcagt
     1021 gcggaaagat tgcaatgttt ttaatggttc gcctgccgga tgtttttgcc ctttttctgg
     1081 actgacggtg gtgaaaatac agcggctaca aggtttaacc acatcaaaca ccacatcacc
     1141 aatgcgaatc actttccagc tatcttcttc ccaggctgac gcgccggaaa ccaccagatt
     1201 ggggcggaat tgctccattt ttacactggc cggacaacgt tgttggagat cacgtaacga
     1261 ggcttcgtta gcaagaaggt aaggatagcc atcagcaaat gacaggggta cagtgttgtg
     1321 gcgtttcacg cgccgcgtca tttgtggccc cacccagcgc aactgcactt cgcgggagaa
     1381 aaatccactt agccatttgt tgatcgcgtc tggtgcaatt cgcgcggtaa aatgtgtgcc
     1441 ccaaacttcg gttggtgcgt cttgtgtggc gaaatcagca aaacgaacat atgcgctact
     1501 gccatctggt gcggtgagat gcaagccatc atgcacaggt gaaggagtaa accgtaccat
     1561 ctggggaaac tggcgagcgg taataaacgt accgtcaggt tccgtgatca taaagatgcg
     1621 atcgaaggcc agaccactga catctgccag ggcatgtgta agaccaatgc cgcgcatcga
     1681 tttaacagga tgaataaaaa gccgggttaa tgtcgccacg gtgcggtcct caaatgaaaa
     1741 taagccctca actttatgac atgacgcgct tattagctat aatgcgcaac aattttctta
     1801 gcgcctgtcc cattaggcta ttttatttgt cagtttggcc cagggaagtg ctcgaaatcc
     1861 tcacgtactt gtgtacgctc cggtttctcc gcgctgtcca tgtccagact gactgcaaca
     1921 attacgccta ctgcggtagg ctcttagagt aaaagtgacg atatgaattc tctgtttgcc
     1981 agtacggccc gtgggctgga agagctgtta aaaactgaac tggaaaacct gggggccgtt
     2041 gaatgccagg tggttcaggg tggggtccat ttcaagggcg acacacggct tgtttaccag
     2101 agcctgatgt ggagccgcct ggcctcgcgt attatgttgc cgctgggcga gtgtaaggtt
     2161 tacagcgatt tagacctcta tctcggtgtt caggcgatca actggacaga gatgtttaat
     2221 cctggcgcga ccttcgctgt ccacttcagt ggtttgaatg acaccatccg caacagtcag
     2281 tacggcgcga tgaaagtgaa agacgcgatc gtcgatgctt tcacgcggaa aaatctgccg
     2341 cgtccaaatg ttgatcgcga tgcgccggat atccgcgtta acgtctggct gcataaagaa
     2401 accgccagta ttgcccttga tctcagtggt gatggtttac atctgcgtgg ctatcgcgat
     2461 cgtgctggta ttgcgccgat caaagaaacc ctggcagccg cgattgttat gcgatccggc
     2521 tggcagccag gaacaccgct gctcgatccg atgtgtggtt ccggtacgtt gttgattgaa
     2581 gcggcgatgc tggcgaccga tcgcgcacca ggcttgcacc gtggacgttg gggctttagc
     2641 ggctgggcac agcatgatga agccatctgg caggaagtga aagcagaagc gcaaacccgc
     2701 gcccgtaaag gcctggctga gtatagctct catttttacg gttcggacag cgacgcacgg
     2761 gtgattcaac gtgcgcgcac taacgcccgt cttgcgggga ttggtgaact gatcaccttt
     2821 gaagtgaaag atgtcgcgca actgaccaat ccgctgccga aagggccgta cggtacagtg
     2881 ttgagcaacc cgccatacgg tgaacgtctg gacagcgaac cggcgctgat tgcgctgcat
     2941 agtctgctgg gccgaatcat gaaaaaccag ttcggcggct ggaatctctc tttattcagt
     3001 gcctcgccgg atctgttaag ttgcctgcag ttgcgtgcag acaaacagta caaagcgaaa
     3061 aacggtccat tagactgcgt gcagaaaaat taccacgttg ccgaaagcac accagacagc
     3121 aaaccggtaa tggcagcgga agactacgcc aaccgtctgc gtaagaacct caaaaaattc
     3181 gagaagtggg cgcgtcagga aggcattgaa tgctaccgcc tgtacgacgc cgatctaccg
     3241 gaatacaacg ttgcagttga ccgctacgcc gattgggtgg tggtgcagga gtatgcgccg
     3301 ccaaaaacta ttgatgcgca caaagcgcgt cagcgtctgt tcgatattat cgctgcaacc
     3361 atttcggtat tggggattgc gccaaacaaa ctggtgctga aaacccgtga acgccagaag
     3421 ggcaaaaatc agtaccagaa actgggcgag aagggcgagt ttctcgaagt taccgaatat
     3481 aacgctcact tgtgggtgaa cctgacggat tacctcgata ccggtctgtt cctcgatcac
     3541 cgtatcgccc gtcgtatgct cggtcagatg agcaaaggca aagatttcct caacctgttc
     3601 tcttataccg gcagcgccac cgtgcacgcg ggattaggcg gtgcacgcag cactaccacc
     3661 gtggatatgt cgcgtactta tctggagtgg gcagaacgca acctgcgtct gaatggccta
     3721 accgggcgtg cgcatcgcct gattcaggcc gattgcctgg cgtggctgcg tgaggcaaat
     3781 gaacagttcg atctgatttt tatcgatccg ccaaccttct ctaactcaaa acgaatggaa
     3841 gatgcgtttg atgttcagcg cgatcatctg gcgctgatga aagatttgaa acgtctgctg
     3901 cgtgctggtg ggacgatcat gttctcgaac aacaaacgtg gcttccgtat ggatcttgac
     3961 ggactggcaa aactgggact gaaagcacaa gaaattacgc aaaaaacgct ctcccaggat
     4021 ttcgcccgta accgccagat ccacaactgc tggctgatta ccgcagcctg aaaggaatag
     4081 taatgtcatt aatcagtatg catggcgcat ggctgtcgtt cagcgacgcg ccgcttctcg
     4141 ataacgcaga actgcatatc gaagataacg aacgtgtttg tctggtgggc cgcaacggcg
     4201 caggcaaatc gacgttaatg aaaatcctca accgtgaaca agggctggat gacggtcgca
     4261 ttatttacga gcaagatttg attgtagctc gtctgcaaca ggatccgccg cgtaacgttg
     4321 aggggagtgt ttatgatttc gttgccgaag gcattgaaga acaagcggaa tacctgaaac
     4381 gctatcacga tatttcgcgc ctggtgatga acgacccgag cgagaaaaat ctcaacgaac
     4441 tggcgaaggt tcaggaacag ttggatcacc acaacctgtg gcagctggaa aaccgcatta
     4501 acgaagtgct ggcgcaactg gggttagatc ctaacgttgc gctgtcgtcg ctttccggcg
     4561 gctggttgcg taaagcggca ttaggacgcg cgctggtgag caatccgcgc gtattgctgc
     4621 tcgacgaacc gactaaccac ctggatatcg aaaccatcga ctggctggaa ggattcctta
     4681 aaaccttcaa tgggacgata attttcatct cccacgaccg ttcgtttatc cgcaatatgg
     4741 cgacgcgcat tgttgatctc gatcgcggca agcttgtgac ctatcctggg aattacgacc
     4801 agtacctgct ggaaaaagaa gaagccctgc gcgtggaaga actacaaaat gccgagttcg
     4861 atcgcaaact ggcgcaggaa gaggtgtgga tccgccaggg gatcaaagca cgccgtaccc
     4921 gtaatgaagg ccgcgtacgc gccctgaaag caatgcgtcg tgagcgtggc gaacgtcgcg
     4981 aagtgatggg taccgcaaag atgcaggtgg aagaggccag ccgctccggt aagatcgttt
     5041 tcgaaatgga agacgtttgc taccaggttg acggtaagca actggtgaaa gatttttctg
     5101 cccaggttct acgtggcgac aaaattgccc tgattggtcc gaatggatgc ggcaaaacca
     5161 cgctgctgaa actgatgctc ggtcagcttc aggcggacag cgggcgtatt cacgttggca
     5221 ccaaactgga agtggcttat ttcgatcagc accgtgcgga actggatccc gataaaacgg
     5281 tgatggataa ccttgctgaa ggtaagcaag aggtgatggt taacggcaag ccacgtcatg
     5341 ttttgggcta tttgcaggac ttcctgttcc atccgaaacg ggcgatgacg ccggtacgtg
     5401 cgctttctgg cggtgagcgt aaccgcttgc tgttggcgcg tttgttcctc aaaccaagca
     5461 acttattgat tcttgacgaa ccgaccaacg atcttgatgt tgaaacgctg gaactgctgg
     5521 aagaactgat cgacagctac cagggcacgg tattgctggt gagccacgat cgtcagtttg
     5581 tcgataacac cgttacagaa tgctggatct tcgaaggcgg cggtaaaatt ggtcgttatg
     5641 tcggcggtta tcatgatgcc cgtggtcagc aagaacagta tgtggcgctc aaacagcctg
     5701 cggtgaaaaa aatcgaagaa gccgccgcgc caaaagcaga aactgtaaaa cgcagcagta
     5761 gcaaactaag ctataaattg cagcgcgaac tggagcagct accgcaattg ctcgaagatc
     5821 tggaggcgaa gctggaagcc ctacagacgc aggtggcgga tgcttccttc ttcagtcagc
     5881 cgcatgagca gacgcaaaaa gtgctcgccg atatggctgc tgcagagcag gagctggagc
     5941 aagcctttga acgctgggag tatcttgaag cgttaaaaaa tggtggctga tcgcaaaata
     6001 gtcgatttaa acagagacag ctgacaacgg taaaattgcc tgatgaactg ggtttatcag
     6061 gcatgaaaac caacactttg ttagtcatct gtaaaatagc gcatcattaa ggagtaccaa
     6121 tgtgcgaaca tcatcatgcc gcgaagcaca tcctgtgctc gcagtgtgac atgctggtgg
     6181 cgttaccgcg ccttgagcat ggtcagaaag cggcatgtcc ccggtgtggc acaacgttaa
     6241 ccgtggcgtg ggatgcccct cggcagcgtc ccaccgccta tgcgttggct gcactgttca
     6301 tgctgttgct gtccaacttg tttccttttg tgaatatgaa cgttgcggga gtcaccagtg
     6361 aaattacatt actggaaatt cccggcgtgc ttttttctga ggactacgcc agcctcggca
     6421 cctttttcct gttgtttgtg caactggttc ccgcgttttg tctgataacc attctgttac
     6481 tggtgaatcg cgcagaatta ccggtccgtt taaaagagca actggcacgg gtgctttttc
     6541 aactcaaaac ctggggaatg gcggagattt tcctcgcggg tgtgctggtc agtttcgtta
     6601 aactgatggc ttacggcagc attggcgtag gcagcagctt tctcccctgg tgtttatttt
     6661 gtgtcctgca actgcgtgct tttcagtgcg ttgatcgtcg ctggttatgg gacgacatcg
     6721 ccccgatgcc agaactgcgc cagccgctaa aacctggcgt cacggggata cgccaggggt
     6781 tacgttcgtg ctcctgttgt acagcaattc ttccagctga tgatccggtg tgtccgcgct
     6841 gtggtactaa agggtacgtt cgtcgtagaa acagcctgca atggacactc gcgctgcttg
     6901 ttacgtccat catgctgtat cttccggcta atattttgcc catcatggtg acggatttat
     6961 taggctcgaa gatgccatcg acgattctcg ctggggtcat cctgttatgg agcgaaggat
     7021 cttatcccgt cgctgcggtt atctttctgg ccagtattat ggtgccaacg ttaaagatga
     7081 tcgccatagc gtggttgtgt tgggatgcca aagggcatgg caagcgcgac agtgaaagaa
     7141 tgcatttgat ttatgaagtt gttgagtttg taggccgctg gtcgatgatt gacgttttcg
     7201 ttatcgcggt gctctcggcg ctggtgcgta tgggaggttt aatgagtatt tatccggcaa
     7261 tgggtgcatt aatgtttgct ttagtcgtca taatgacaat gttttctgct atgacgtttg
     7321 acccgcgttt gtcgtgggat cgtcaacctg aatcagagca tgaggagtcc tgacagtatg
     7381 gaatctaata atggggaagc caaaatccag aaagtgaaga actggtctcc cgtgtggata
     7441 tttcctatcg tcacggcgct cattggggcc tgggttcttt tttatcatta cagccatcag
     7501 gggccggaag tgaccctgat caccgcgaat gcggaaggaa tagaaggtgg taaaaccacc
     7561 attaaaagcc gtagcgttga cgtcggcgtg gttgaaagcg ccacactggc tgatgatttg
     7621 acgcacgttg aaatcaaagc gcggctgaat tccggtatgg aaaaattgct gcataaagat
     7681 accgtctttt gggtggtgaa accgcagatt ggtcgagaag ggattagcgg cctgggaacg
     7741 ctgctgtctg gtgtttatat cgaactgcag ccaggcgcga aaggcagcaa aatggataaa
     7801 tacgatttgc tggactcacc accgttggcc ccgcctgatg cgaaaggtat ccgtgtgatt
     7861 ctcgatagca aaaaagccgg gcagctctcg ccaggagatc ccgtgctgtt ccgtggctat
     7921 cgggtaggtt cggttgaaac cagcaccttt gatacgcaaa aacgcaatat cagctatcaa
     7981 ctgttcatca atgcacctta tgaccgactg gtgaccagca atgttcgctt ctggaaagat
     8041 agtggcattg cggttgattt gacgtcagcg ggaatgcgtg tggagatggg ttcattgaca
     8101 acgctgctta gtggcggtgt cagctttgat gtgccggaag gtctggattt agggcagcca
     8161 gtggcaccga aaacagcttt cgttttgtat gatgatcaga agagcattca ggattcgttg
     8221 tacaccgatc acattgatta tctgatgttc tttaaagatt cggtacgcgg tctgcaaccg
     8281 ggagctccgg tagaattccg gggtattcgc ctgggtaccg taagcaaagt gccattcttt
     8341 gcgccgaata tgcgtcagac atttaacgat gattaccgta ttccggtact gattcgtatc
     8401 gagccagagc ggctgaaaat gcagcttggc gaaaatgcgg atgttgttga gcaccttggc
     8461 gaattgttga aacgtggttt acgcggatcg ctgaaaaccg gaaacctggt cactggcgca
     8521 ctgtatgttg atctcgattt ctatccaaat acgcctgcaa taaccggtat tcgtgaattt
     8581 aatggttatc agattatccc gaccgttagc ggcggtctgg cgcaaatcca gcaacgactg
     8641 atggaagcgt tggataagat caacaaactg ccattgaatc cgatgattga acaggcaacc
     8701 agtacgcttt ctgaaagtca gcgcacaatg aaaaacctgc aaacgacgct ggatagcatg
     8761 aacaagatcc tcgccagcca gtcgatgcag cagctgccga cggatatgca gtcaacgttg
     8821 cgtgaattga atcgcagcat gcagggcttc cagcctggct ccgcagccta caacaagatg
     8881 gtggcggata tgcagcgcct tgatcaggtg ttgcgagaac tgcaaccggt gctgaaaacg
     8941 ctcaatgaga agagtaacgc gctggtattt gaagcgaagg acaaaaaaga tccagagccg
     9001 aagagggcga aacaatgaaa aagtggctag tgacgattgc ggcactgtgg ctggccggat
     9061 gcagctccgg cgaaattaat aaaaactatt accagttacc tgtggtgcag agcggtacac
     9121 aaagcaccgc cagccagggt aatcgcctgt tatgggttga gcaggtcgct gttcctgact
     9181 atctggcggg gaatggtgtg gtttatcaaa ccagtgatgt gaagtatgtg attgccaaca
     9241 acaacctgtg ggccagcccg ttggatcaac agttgcgcaa cactctggtt gccaacctga
     9301 gcacgcaact gcccggctgg gtggttgcct cccagcctct gggaagcgcc caggatacgc
     9361 tcaatgttac cgtaacggag tttaacgggc gatatgatgg caaggtcatt gtcagtggtg
     9421 agtggctgtt gaaccaccag ggacaactga tcaaacgtcc gttccgtctg gaaggggtgc
     9481 aaactcagga tggttacgat gaaatggtta aagtgctggc cagtgtctgg agtcaggaag
     9541 ccgcttctat tgcgcaagag ataaagcgtc taccgtaatc ataaagattt gtaaatataa
     9601 ccgtctccgg tatgttgcct gaggcggttt ttttgtttct aaggtgcgga aaaagttgtt
     9661 cctcttcaca ttttttgtac aactgacatg cccgtgtagc tcacaaatat gacagtggcg
     9721 tgaattttgc gcattgacgg cagttatgat tcgcggtatt gcttaactgt gattgcacat
     9781 ttagtaatca ctgttttctt ttccaccaga aaccagtatg agggaaacga ggcatgaaga
     9841 gacaaaaacg agatcgcctg gaacgggcac atcaacgtgg ttatcaggcc ggcatcgccg
     9901 gacgctcaaa agaaatgtgt ccctatcaga cgctgaatca aaggtcacaa tggctgggag
     9961 gctggcgaga agccatggcg gacagggtag taatggcctg attctgtctc tttaaaaaga
    10021 aacctccgca ttgcggaggt ttcgcctttt gatactctgt ctgattataa tcagaaggca
    10081 gacgtatcct ggaacagacc gactttcagg tcgctggcgg tatagatcag acgaccatca
    10141 accagcactt cgccatctgc cagacccata atcagacgtc gattaacaat gcgtttaaag
    10201 tgaatacggt aggtcacttt tttcgctgtc ggcagtacct gaccggtgaa tttcacttcg
    10261 ccaacgccca gcgcgcggcc tttaccttcg ccgcccagcc agccgaggta gaaccctacc
    10321 agctgccaca ttgcgtccag gcccaggcat cccggcataa ccggatcgcc aatgaagtgg
    10381 catccgaaga accacagatc cggattgata tccagttctg cttcaacata ccctttgtcg
    10441 aagttaccac ccgtttcggt cattttgacc acacggtcca tcatcagcat gttcggtgct
    10501 ggcaattgcg ggcctttagc gccaaacagt tcaccgcgac cagaggcaag aaggtcttct
    10561 tttgtatagg attcgcgttt atctaccatg ttctctgtaa gccttatttt attgaagcac
    10621 gcaggatagc taacacgtgt acgctgaaca agtccgatca gttcggaata aaccagttca
    10681 gccaacgcaa tggccatgga aaacggtgac gtccttcctg ttgcgatgct tgcgcgatac
    10741 gttcctggat ggtttgcatc agcgtcgttt ggccttcgcc atcccacacc agatttaata
    10801 ataacggcag tgcgtcagtc acatcgtcta ctgcccagat ggtgaatttg ccttcttcta
    10861 ccgctttcac cagttcactg tgaagactta aatggcgaat gttagcagtg gggataatga
    10921 caccttgttt cccggttaac tcacgttgct ggcaaatagc aaagaagcct tcgattttct
    10981 catttaaacc accgaccggc tgggcacgac cgaactgatc gactgaacct gtgatagcga
    11041 tactctgatt caccggcaca tcggcgaggg cgcttatcag ggcgcagagt tcagccatcg
    11101 aggcactatc gccatccact tcactgtatg actgctcaaa tgtcagcgat gctgagaagg
    11161 ggatctgttg ctcaagctgt agttccgaca tcaggaacgc ctgcatgatc atcatccctt
    11221 tcgcatggat attgccgcca agctccgctt tgcgttcgat gtcggtgaac tcaccatcgc
    11281 caatatgcac aacgcagcta atgcgagaag gttcgccaaa agcgcgtgga tgacccggaa
    11341 attcaatgac cgaaagggcg ttaatttgcc cgatgcgttc gccttcggtt tcaatcagga
    11401 tttgctcctg aagaatctca tcctgcatgc gttcagcgag gaaaccttca cgccattcac
    11461 gctgctgcag cattaagttt agctgctcgc cggagaaggt gtcgccatcg cacagggagg
    11521 cgacctcttt acactggcgg aggatccact gcgggctaag cggaagtgtt tcttgttcac
    11581 cggtgtagcg ggctgcttcg cggataagta ccggccaggc atccgctccc ggtgcaggta
    11641 agtgattatg tcttgcggtc aatgttaccc agcgacacca ctggcttact gactccgcat
    11701 cgacaatctg cagagtatct tcaaattcgc tataaatagc ctgctctgaa agctctggtt
    11761 ccatctcctg gaaatcagcc aatgattcgc gttcgcctac cagaatgact ttcagcttca
    11821 atggcatcga aggcacagag acggggagag ggcgcgactc atcaaacgca acccagtcaa
    11881 aacgctcgcg gttaacgata tttttcagcc gcatccacag cagaggttgc gccagcagtg
    11941 tacgcaaaga gatgatgaga ataccgccat ttgcctgatg caccagacct ggctgcaggg
    12001 taatgtcgcc attaaactgg cgcaggcagc caaagagttg ttccgcttct acccagtcgg
    12061 cagcgacaac ttgcgttaaa gtcgcaaaat tatcatctgc actcactgcg ttacgtaagc
    12121 ggatggtgtg cccggaaacc tcatagtgac cgcccaccag ttggcctgca tcgctttgta
    12181 gcgcacgcgc ggcatcggca ataagattca gatactcaga ctcttccggg gccttcgcca
    12241 gcataaaaga ggaggatgct cgcgtatgca gcaactgctc cagcgcaaat tgcagtcgcg
    12301 gttgagtatc actgaataaa ggatcgtttt cgtcaatcaa atgtggctga gcaaatattt
    12361 cctgatagct atcggtatca ggaaccaggt cacgccatgc aagtttcgta atggtcaaag
    12421 ttgatgtttt ttagtctgtt gtcaaagccg cgattatacc gtaaccggca ctacagcaca
    12481 cgtagaaagc accgacaata ctcctggcat gggcgttaaa gctcacagga tggagattct
    12541 tttcttcact ggcccaaaaa gctgatattc tgtaaagagt tacacggtaa cattgagatc
    12601 gctatgaaat atcaacaact tgaaaatctt gaaagcggtt ggaaatggaa gtatctggtg
    12661 aagaagcacc gcgaagggga gttaatcacc cgttacatag aagccagtgc cgcccaggaa
    12721 gccgtcgatg tgttgctctc gctggaaaat gaacccgtgc tggtaaatgg ctggattgac
    12781 aagcatatga atccggaact ggtcaatcgc atgaagcaaa ccattcgggc cagacggaag
    12841 cgccatttta atgcggaaca tcagcatacg cgcaaaaaat cgatcgatct ggaatttatt
    12901 gtctggcaac gtctggctgg tcttgcgcag cgtcgcggta aaacgctttc tgaaacgatt
    12961 gttcagctga ttgaagatgc ggaaaacaaa gagaaatacg cgaataaaat gtcttctttg
    13021 aagcaggatc tgcaggcatt gctgggtaag gaataactga cgaaagtcag ttcaattttc
    13081 taaagacgaa aaaaaacccc gcagcagcgg ggttttttac cagacgagaa cttaagcctg
    13141 cggctgagtt acaacgtctt tgatgccttt aacttcgatc tctacgcgac gatccggagc
    13201 caggcaatcg atcagtgcag cacgctgttt cacgttgtca caggtgttgc cagtaaccgg
    13261 gttggattcg cccataccac gtgcggagat tttgtctgcc gggatacctt tagagatcag
    13321 gtaatcaaca acggactgag cacgacgctc ggacagagcc tggttataag cgtcagaacc
    13381 gatgcggtca gtgtaaccca gaacaactac ggaaccgtct ttcggatcca ggttgctcag
    13441 ctggctgtac agctgatcca gagcagcctg accttccggt ttcagggttg ctttgttgaa
    13501 ggtgaacaga acgtcagact tcagagtgaa gtgcttggtc tgtacttccg gtgccggagc
    13561 tggagccgga gcaactactg gagctgcttc gccctgaccg aaacggtagg aaacacccag
    13621 gctcagcatg ccgttgtccg gacgagtgcc gatggtgtgt gcgtcaccga tgttgttggt
    13681 ccactggtat tccagacggg tagcgatttc aggagtgatc gcatactcaa caccgccagc
    13741 gaagaccgga gaaacgccgg tgtcgtggtc tttagtggat gcgccaccag gtacgttaga
    13801 cttggtgtct gcacgccata ccataccacc cagacgagtg taaacgtcca gatcgtcagt
    13861 gattgggtaa cccagtttag cggtcagctg aacgccctga gctttgtatg cgccgttgat
    13921 gttgtcgcct ttgtacggca tacgacctaa ccagtcgtaa cccatttcaa agccaacata
    13981 cgggttaacc tggtaaccac caaaagcacc tgcacccagt tggttttcgt gggtcggacc
    14041 attgttagga ataaaaccag tgtcatggta ctgggaccag cccagtttag caccagtgta
    14101 ccaggtgtta tctttcggag cggcctgcgc tacggtagcg aaaccagcca gtgccactgc
    14161 aatcgcgata gctgtctttt tcattttttg cgcctcgtta tcatccaaaa tacgccatga
    14221 atatctccaa cgagataaca cggttaaatc cttcaccggg gatctgctca atattaactc
    14281 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    14341 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtacgt
    14401 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    14461 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    14521 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    14581 gtctcgttgc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    14641 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    14701 cgatcaccac gctgtaattg cccgtgcgta aagcgcgaac cattgactca acggtgtggc
    14761 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    14821 attcgcgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    14881 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    14941 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    15001 ctgcggatga gaacgacgaa gcacgatgtg catagcctga agtgtacata atcaatccag
    15061 ccctgtgggt tactgtatga atatacagta catccggtga cgacaaagat caaccctatt
    15121 ttcggaaaga gcctcgcaaa ttttgtcgtt agtgacggga aaacataaat taatcttgcc
    15181 ccttaagaat aagttgccta ttttcgtagt taacggatcc gttaatgtga atcattcttt
    15241 tatgttatga ttttaaaagg aattttatga aaagcctctc ctataagcgg atctataaat
    15301 cacaagaata cctggcaacg ttgggcacaa ttgaataccg atcattgttt ggcagttaca
    15361 gcctgaccgt tgacgacacg gtgtttgcga tggtttctga tggtgagttg tatcttcggg
    15421 cttgtgagca aagtgcacag tactgtgtaa aacatccgcc tgtctggctg acatataaaa
    15481 agtgtggccg atccgttacc ctcaattact atcgggttga tgaaagtcta tggcgaaatc
    15541 aactgaagct ggtgcgtctg tcgaaatatt ctctcgatgc tgcgctgaaa gagaaaagca
    15601 cgcgcaatac ccgggaaaga ctgaaagatt tgcccaatat gtcttttcat ctggaagcga
    15661 ttctcgggga ggtggggatt aaggatgtac gggcgttacg tatacttggg gcaaaaatgt
    15721 gttggttgcg actgcggcag caaaacagtc tggtgacaga aaagattctg tttatgcttg
    15781 aaggggccat tatcggcatt catgaagctg cgctcccggt ggcacgccgc caggagcttg
    15841 cagaatgggc tgactctctt acgccgaaac aggagtttcc tgcggaactt gagtaatctg
    15901 gcgttgcaga cgaccaatct ctggcagtaa tgcaatcaat aacccaactt gttgtacgac
    15961 cagaggttct ttgctgtctg cccgtggttc aagttgctgc atccgctgtt tcaggccagc
    16021 taatgcctga ttgacgcgtt cttcatcagc aggttgatga tgtaacgcgt catcaacata
    16081 gcaaactgcg tcatcaagaa acgccagaat ttcaggatta gttaactgct cccggtgagc
    16141 accgagggct gagatatagc tggtaaacgt atggttaagg cacagcaacc gaaacgcggc
    16201 ttcgcgaatt tgcggggtaa cgttcggttc gctggacata tttgatacca ccgacgccag
    16261 ttcagcatca cggttgtgtg catcgcggcg ggcaatacga tacgccagac ggttatcacg
    16321 cccctgatgg tattgctcca gtatggcatc gagataccgg cagttggcct cggtggcgcg
    16381 ttcgagcatt cgcggcagat tgcgaaactt ccagtcaggc cagatgtagc tcactgccgc
    16441 ccacgcaatg gcacaaccaa tcagcgtatc gattacgcga ggtaacgcca cttcaaaacc
    16501 ttcacccagc aagttgaaac acagtagcac caaaagtgtg atgaacatcg ttgcatgggc
    16561 gtattgcacg ttacggaagg caaaaaagag cacgccggta ataaccagca gcaccagctg
    16621 cccttccagt gatggcacaa accacagcac aggaatacca atcgcgatac ctaccagcgt
    16681 accaataatc cttaacttca ggcggtggcg cgtggcgtta tagtttggct ggcagacaaa
    16741 caaacttgtc agcaagatcc aatacccgtg atgcattccg gttatctgaa tgatggcgta
    16801 gccgaagcac aacaccagcg acattcttac cgcatgacgg aagagggcgg attccggcgt
    16861 gaagtgacgg ctaagacgca gccagatatc actcaatccg tgcgggctgt catcagcgag
    16921 ctcattttcg tcattattat ggggcagtgc ctgggcctgt tctgattcaa ttgttgccag
    16981 ttgggcatca atggcacgta aattgttcag taaaaatcct agtgttttga gtaaatcagc
    17041 aggagcgccg ttatcgcgca tccgctccag cgcagcatca atatgcgtaa aagcgcgctc
    17101 aaaatgcgga tcatgttgat aaggctgacg cagcaaaata cagcgtgaca gttgctggca
    17161 cgcctggccc tgcatcgaca tcagccgctg aaaacggaac agcacgtcgc tgtggcgaaa
    17221 atgttcacgc aatgtttgat attgaatatg agaagagctg gcacgctcgt gaatatcctg
    17281 cgcgacaaag taataatgca gtgtgcgacg cgttcctcgt tgaccacgat cgccacgtaa
    17341 gcgggtcagc agcgagagtt tcgtctgatt caatgtcgcc atcagctgac cgttggcgag
    17401 agccaaatcg tacagcggtg cctggctttc atcttcaata tcaggatcaa acatgcgcga
    17461 cttgagctca agataacgcg ccagttgttc atagcaacgc gccaggttgt cctgcagcgg
    17521 gcggaccggg aacagcagat gaccaataag tgtcaggacg ttgtaccaga cggcaccggc
    17581 cagcagatac atcggctgct gataccagtg ctcatacagt gacgtcccca acatagtgta
    17641 aatggcgatc agtaacgcac cgaaggcaat tgttgcatag cgttgaccca gaccgccgag
    17701 caaaatgaag ccgctggtag agagcgttaa accaattgca aatagccagg gccagggaaa
    17761 cagcaattct actgaggccg aggcgataaa aaagcagaac agcgtaatga tgaggttacg
    17821 caaacgtccc gccagtcggt catcgagatc ggtcagcgcc gctgccacca tccccagcgt
    17881 tagcggaatc gtcagtttta catcacccag ccaccacgga aacgctgtgg ttccacaaag
    17941 cgcaataaaa atacgcgcgt aatacagcca ggcgctgttc caggtatagc gtttgagcaa
    18001 aggacttagc ataaaggcca tattagtgga ttattcaaaa cgacgacgtg cattggcttc
    18061 acgcgcagct tgcgctgttt ctaccgatac cacgcgacga ccgaccggcc atagtgcaat
    18121 agcggcaatt ttaaagttcg caatgccgac aggaatgcca atgatggaaa tacattgtgc
    18181 gatgcccgtt gcaatgtgca tcaggcataa ccaccagcca aagaaaatca gccagaaaat
    18241 attcaatacc gtaccgccag tatttagcag cacatttttg ccagccgggt tcagttcatc
    18301 gacatgaata gcttcattgc cataaggcac cagagacagt ttagtgatct cccagcagga
    18361 tcgtgtcagc ggtaaggtaa aaatcagcac aatactgacc agagtcgcca acagccagcc
    18421 cagagtggtg gcaaatccgc caagcacaaa gttcagaatg ttcaaaacgg tacgcataaa
    18481 acctcgcttt actgtggttt tcagtaatgg gcggcaattg taacgttttt ttgggctgga
    18541 gcacgttttc tctgacggtt acactgataa gaaataattt cgtgtggatc tacagagtca
    18601 tggaactgaa agcgacaacg cttggaaaac gtctggcaca gcacccttac gatcgggcgg
    18661 tgatcctcaa tgccgggatt aaagtctccg gcgatcgcca cgaatacctt attcctttca
    18721 atcaattact ggcgattcac tgtaagcgcg gtctggtatg gggcgagctg gaatttgtac
    18781 tgccgggcga aaaagtggtg cgtctgcacg gtaccgaatg gggcgagacg cagcgttttt
    18841 accatcatct tgatgctcac tggcagcggt ggagtagcga gatgagcgaa attgcatctg
    18901 gtgttttacg ccagcaactg gatttgattg ccacgcgtac tggagaaaat aaatggctga
    18961 cgcgtgagca aacctctggc gttcagcaac aaatccgcca ggctttgtcg gcgttgccgt
    19021 tgccggttaa ccgactggaa gaattcgata actgccgtga ggcatggcgt aaatgtcagg
    19081 cctggttgaa agatattgaa agcgctcggt tgcagcataa ccaggcgtat accgaagcca
    19141 tgcttaccga gtatgcggat tttttccgcc aggtcgagtc ttcaccgctg aatccggcgc
    19201 aggcccgggc agtcgttaat ggcgagcatt ctctgttagt gctggctggt gcaggaagcg
    19261 gaaaaacgtc ggtgctggtg gcccgtgcag gctggttgct ggcgcgtggt gaagcgtccc
    19321 ctgagcaaat tttattgctg gcgtttggtc gcaaagccgc tgaagagatg gacgagcgta
    19381 ttcgagaacg actacatacc gaagacatta ccgcacgcac atttcatgcg ctggcgctgc
    19441 atattattca gcagggcagc aaaaaagttc cgatagtcag caaactggaa aatgataccg
    19501 ctgcccgtca tgaacttttt attgctgagt ggcgcaagca atgcagcgaa aagaaagcgc
    19561 aggcgaaggg ctggcggcaa tggctgacgg aagaaatgca gtggtcagtg ccagaaggta
    19621 atttctggga tgatgaaaaa ttacagcgtc gtcttgcctc tcgcctcgat cgctgggtaa
    19681 gtctgatgcg gatgcacggt ggtgcacagg cagaaatgat tgccagtgca cccgaagtga
    19741 ttcgcgatct gttcagtaaa cgtatcaagt tgatggcccc gttattaaaa gcctggaaag
    19801 gtgcgctgaa ggcagaaaac gctgtcgatt tttcgggcct tattcaccag gcgattgtga
    19861 ttctggagaa aggtcgcttt atcagcccgt ggaagcatat tctggttgat gaatttcagg
    19921 atatctcgcc gcagcgcgca gcgttgttag cggcattacg caagcaaaac agtcagacga
    19981 cgttgttcgc cgttggtgat gactggcagg cgatttatcg cttcagcggt gcgcaaatgt
    20041 cgctcaccac cgctttccat gaaaactttg gtgaaggtga tcgctgcgat ttagacacga
    20101 cttaccgttt taacagtcgt atcggtgagg tggcaaaccg gtttattcag cagaacccag
    20161 gccagctgaa aaagccgcta aacagcttaa ccaatggaga caaaaaagcc gtcacgttat
    20221 tggatgagag ccatcttgac gctttgctgg ataagctctc tggttatgcc aaaccggaag
    20281 agcgcattct gatcctggcg cgttaccatc acatgaggcc tgccagcctg gaaaaagcgg
    20341 caacacgctg gccgaagttg caaatcgact ttatgaccat tcatgccagc aaagggcaac
    20401 aggcggatta cgtcatcatc gttggcttgc aggaggggag tgatggtttt ccggctgcgg
    20461 cgcgggagtc gattatggaa gaggcgctac tgccatcggt tgaggatttc ccggacgctg
    20521 aagaacggcg gttaatgtac gtggcgctga cccgggcacg ccatcgggta tgggcactgt
    20581 ttaacaaaga gaatccctct ccatttgtcg aaatactgaa aaatcttgat gtgccggtgg
    20641 cgagaaaacc gtaagaaaca ggtggcgttt gccacctgtg caatattact tcagacggtc
    20701 cgcgagataa cgctgataat cggggatcag aatatcgacc gcgtcgttga aatgcggcga
    20761 ctggataatg aagtctgccg ttgccacgtt ggtggcgacc ggaatgttcc ataccgtcgc
    20821 cagacgcagc aaggctttca cgtcagggtc gtgcggcacg gcgtttagtg ggtcccagaa
    20881 gaaaatcaat acatcaattt tcccttctga gatcaatgcg ccaacctgct ggtcaccccc
    20941 cattgggcca ctcaacatcg cgttgacgtt catgccggtt gcacgggaaa ttaagttacc
    21001 ggtagtgcct gttgcataca gtacgtgttg ttccagtaac ggttgatgcc gttccaccca
    21061 gctcatcagc atttgtttgc agtgatcgtg tgccaccagc gcaatatgtt tccgcgcagg
    21121 taaagtgcgt gtcgtcagtt ccataatgta catccgtagt taactttcct acagattact
    21181 gtaagcactt atcgctgcaa gataaagacc gaaaaagcct gcgcacaggc acaaaaatct
    21241 caggaagatg gttgtttttc cgcccattgc aggaaagtat ttcgcgtttg tgggtcagcc
    21301 agtttaaacc aatacttcag ccgttgttct gtgagcacct gagactgcgg tggaatagca
    21361 tccagctcgg aaacacccga aagcagcgta ctatcatctg ccatcatcgt ggcaaattgc
    21421 ggtagtgaag cgcgtttcgc ggatttgtta tagcgttcaa cctcgacctc ataatcaatc
    21481 gtttttgccg ttgaggtaat ggcgagaata tccagcttta ccggaatcgg cgtcgcatcg
    21541 ccatccaaca attcaaggcg cggcgcggca tcaaaatggt tagcttcccg ctcattctcc
    21601 agacgaggca ggcgaaaatt gacctggttg atgagctggg tattaaaact gaccaccagc
    21661 ggtggggaga tatagagccg ttcttcacta ttggaaagat gaatcgtctt ctcaacgcga
    21721 aacactaact gatgcggtcc gttatccagt tcaatgctgt ccgcaccgcg cagcagagag
    21781 ctggaaacct tcttgccgtc gagcaccaga agatcgacat cggttgaaag ccgcagcgtg
    21841 gtggcaaaaa cggataccgg caaacataat gcaatcaagg tggtcacgat gccggttttc
    21901 ataggtagct cctgtcaaaa gaccgcccga ataataatgt gtcaaaattt ttctttaatc
    21961 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
    22021 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
    22081 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
    22141 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
    22201 gtcattccgg tttcgccaaa agttgctggc aaaacactgc tgggccagca ggggtatggc
    22261 acgctggcag aagttcctga aaaagtcgat atggtcgatg ttttccggaa ttcagaagcg
    22321 gcgtggggcg tagcgcagga agcgatcgcc atcggcgcga aaaccctctg gatgcaactg
    22381 ggggtgatta atgagcaagc ggcagtgctg gctcgggatg ccggattaaa tgtagtgatg
    22441 gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga
    22501 aggcaaaaac cttccgggga tttgttcagg gattagttac gcagacgcgg ggcctggagt
    22561 tgtttacgga tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc
    22621 aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc
    22681 tccatcacca cgtgatacca cggagcggcg cgaagctcgt cattcaccgc cagctcatca
    22741 ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct
    22801 aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc
    22861 cgggaaatct ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga
    22921 caggcaaacc ctttcagata tagcccttcc ggataggtag cgatcaccgg atgatcggct
    22981 gcctgacgga actgctctat aaattgtaca tcacgaccgg catcaattgc ggcatccgcg
    23041 atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata
    23101 ccgccttcat tcagcagctg aatcgccagc atgttgatgt ctttataacc acgacacgcg
    23161 cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat
    23221 ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca
    23281 gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc cagggcttcc
    23341 tgggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa
    23401 ccaccggtat aggagaaaca gttaagtacg cgtttatttt cgacgtagcg gcgggtagcc
    23461 aggcggctgt cacgctgatc caggtagtag cccgttttgt gtccgtgctg aatattcacc
    23521 agcagtttca tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgatg
    23581 gggccctgag tcagctccat cccttctttt ttacgtaccg cgacgtcgct gcgatcgtaa
    23641 atggcacatt ccgggtacag cgtttgcagg gcgctaatta atgccgcgcg ctgatactct
    23701 gcgccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc
    23761 ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc
    23821 agccagtcac gccatttttg tgcttgctgc aaacggcggg taaaaaaagc aatgtcgata
    23881 gattcattcg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg
    23941 ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct
    24001 ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat
    24061 ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca
    24121 aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata
    24181 aggagagcag caatgtcgaa agtctgcata attgcctggg tttacggtcg agttcagggc
    24241 gttggatttc gctataccac gcagtacgaa gcgaaaaaac tgggattagc cgggtacgcc
    24301 aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtagaa
    24361 aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc
    24421 agcgagccgc atcatccctc gggggaatta accgattttc gtatccatta aatacatttt
    24481 accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt
    24541 cggtataaat agcggctgtt tcctttttct tcgccaaatt tattcgccat cgcttttacc
    24601 agcatacgaa tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc
    24661 acttcccagt gttctggcga cagcgtaatc ccttcgttct ctgcaatcac cactgccagc
    24721 ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta
    24781 ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa
    24841 acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtaagatta atcgcggctg
    24901 gcgaagccca gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta
    24961 accgtggcac gaatatagtt cgtttcaccg ccatgaatga tgttgctggt ttcaaacaag
    25021 atagcgccag aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagt
    25081 tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc catcagcata
    25141 ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcggagca gcagaagaac
    25201 actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag
    25261 gtattcagaa ttggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata
    25321 atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc
    25381 agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc
    25441 gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg
    25501 ctaagcagcg atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct
    25561 atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt
    25621 acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag gttattaagc
    25681 aatgatataa cgctctgaga atttcgacag acaaaagaaa aaggggttag catttagcta
    25741 accccttatc ttatttggcg gaagcgcaga gattcgaact ctggaaccct ttcgggtcgc
    25801 cggttttcaa gaccgaagaa aaacaaataa aaatcagtgt aatacgtgaa gctgctgaaa
    25861 atttaatcaa atttaacacc aagaaaaatc aaggtgttag gaagttgctc atagctagta
    25921 taccaagtat ttttgccaga aaattttctg tattgcctac ttgctaacca cgagcggcag
    25981 atccagtgtt ggtgtgattt ttgttttacg atcgtaaatc aacacctgat tttctgtttt
    26041 atgcccgctg aaaatttgtt tgtcgcgact gctgccttcg taatcagaga tccccttggc
    26101 ttttatatcg tgaaaattgc acccgaacgg aacgccaact ttttgctcgg ctgagcgttt
    26161 agcctgattc caccagttgt tcagcgtttt agctatgact ttcccgcctt tggttgtatt
    26221 gatcacatat tcgcatgtac aggaagatac atttcgggct aactggatcg ctgtacgtaa
    26281 tcgcggtgac cattccttga tttgtttggt gccggttttg ttttgctcaa tgtagatccc
    26341 tttatccata atatcctgcc atttcaactc aagcacatca ccgagccttg ccgcacagag
    26401 ataggaaatc tccattgcaa tgcgtaactg tggaattgct tccgcatata tcgctgcata
    26461 ctgttcatcg gtgatgtaaa cagtgcgggc tttaagagag aattttctga ctcctttgca
    26521 tgggttattc ttcacgtatc cacgctcata tccccacccg tatactcgac tcaggcttgc
    26581 cagttcatga tttgcctggg tcttgctctc aagccctcgt ttatccatga aaattcttac
    26641 ctgctcagtt ttgacattat cagcaagcac ttttccgaat accatcagca acgccttctg
    26701 atgttgtcga taatcttttt gggttcgggg ggccagttct gtaaatgcag gggagtccat
    26761 aaacatgtgc cataatttag ctacggtcat tatgttgtgg agttttgctt tttccagttc
    26821 ataattttgc cagactttag ctacgctggt tttccgcact cttcctagcc ctatagttct
    26881 tgtacttcct tctggtttcc acacgtaact gtaaccattc gatctaaccc gcggtggcag
    26941 tacattatct tttttatttt ttcttggtct tcccattgtt cagcgcctca aaatcgggtt
    27001 cagcagaaac cagttcagat gtttttggca tcgttgtcag tccgtgtgga atatccctgc
    27061 gtagaactat tggttcgttt ttaggaccga ttacaaatgg gatgccgtgc agtcttaact
    27121 ggtgttgctg ttttgtgtat cgctcgtatt tcgtgatctc tttaatctct gctggagata
    27181 gagttaattc gtacatgtgg tcacgtgcct ttacagcatg accgccgcca ctatagctgg
    27241 tgggcggcga tatgggttga acattaaaaa tcagcctgac tcgggatcag tttttgccag
    27301 atagctgaaa cgtattttgc ctgataacgg gcgtcatcaa gtgcattatg gcgctcacct
    27361 tcgaatggaa tagccgttct ggcatcgaag tctatggctt tccccagctc aacgattgtg
    27421 cgtacatcgc gatcgttgta gtaacgccac gggcagggga tcccctgccg ttcgtatgaa
    27481 cggcgcaaaa tcgtgttgtc gaagttggct ccatttcccc agacctgaac aaaaaattca
    27541 ccggagtttt cgtcgataaa ttcccgcagt tgtaacagtg catcatctaa cgggatttca
    27601 tcggtcataa tggcagattg cgcttcgcgt gattgcttaa gccaccattt aatggtgtcc
    27661 cgatcaatga ctccgccagc agtttccaga tcgatagtct tactaaattc cggtcccata
    27721 tctccggttt gcggatcgaa aaatattgca cctattgaga tgatcggggc attaggattt
    27781 tttcccatgg tttcaaggtc gatcattaga tggtcacacg tcctgctggt ggatgtgatt
    27841 tcgtgatgac cgttcaccgc aattaaggga tctgccgctt cgccagtttc actatcgctg
    27901 gtgtgatgct gatcgttacc agcattctca ttgttcggat gttcagcgcc ttctatttcc
    27961 tccggatcat cttcctgaac ttcagcctga tttttttcat cgaatgtttc ctggtatgtt
    28021 gcgtcgccca tcaccgcgcc acagtcagga cagttgccgc caccgctctg accgcaggcg
    28081 gtgcagactt tttcctcttc ctgttgcgct actggttcgg attgtttcgt ttctggctcg
    28141 tcttgtaacg cattttggct gttttgttct gctttctggt cgttctgttc cgtttctagc
    28201 tggttctggt tcacagaatc gcaggtttca atccccttaa cccatttcga atcattcggg
    28261 tcgctaatcc cttcaacaaa ctcaccacga gaagcggcaa gcaatttgtc ggcatctggt
    28321 cgactgatgt ctgctgcctc cataatttta tttacttctt cagcggtaac ttttacctgt
    28381 gacttgtcat ccagcggctg cgggtcctga tgatgttcaa ctgtatccgc tgccattgtt
    28441 tcagccgttg cctgttcatc tgccattgcg ccagatggtt gcgggttttc ttcatcattt
    28501 ttttcttctt ctgttttgca ctcagcagcc agtccgtcgt taatttcttc caggatatct
    28561 ttttccggtg tatgccgggc agccatgagc gtttcagctg tgggattctc gtgatcagtt
    28621 tctgtcaggt tggcattgat atacccctga aggcgtcccg ggtagtgata aaactcaggg
    28681 tgggcgctcc ggataagtgc aaaaatagct gcgcgggaat agtccaggat accaggggtt
    28741 gcgcgaagtg ctgcggacca ttccctgaac ggactttctt ttttcaggac gatttctttt
    28801 gcgcgacgat aaacgctcac gggtatttca taaatattaa aatccatcgg aagcgtggct
    28861 gctgcagcct ccacatccag agtatcgagg gtgtgtacta agtcaggatt acgatcggtt
    28921 ttatttccgc caccggcatt tgcaccggaa gccgtgcggg tgatgcgaga aacacgattt
    28981 cctttcatcc actcttttgt cagcagacct cgatcggtat agtcagcgtc caggtatgct
    29041 tcgaaaaaag cagtcattaa ccccagactg gaattaacgg gattagggaa aactttgtct
    29101 gtgtcgcgta ccagtttatg aaggtcgcga atctccagcg ggtcgagcag ctttgtgttg
    29161 tgggaaatag ccagggcagt aacagccggc agttcttcag ctcgtgcgat atgtaatgcc
    29221 tgaagttctt cccgtgaaac gtgcgttacc ggtttttcgc tgccatgttg cgcaagccag
    29281 cgaatgggta gttcctgacc ggaaattggc aggagcatat tatcctcaat ctcagccatg
    29341 tcttcgccat tgacgttggt attgtcagtg ctggctggtt tttcctgaac tgagggggaa
    29401 ggcgctataa atatcattgt gatgccatct tccccgcctt tttcatagcg gttgcagaat
    29461 tcagtatcaa acacgccttc cggtggaagg tcattcacaa cggggaaatg gacgcggacg
    29521 ggttttttga aatcatcagc gtcgtagcct gcctcgtcca ttgcaacaac gcaccgcgag
    29581 attgctacag ataatttctt ttctgtactc cagtaaaaac cccctttaat cccaaagcgt
    29641 tttctgacct tgtcattttt tgcttcgcaa tatagcgcaa attcttcttt atcagtgctc
    29701 attgataaac ctcataacta ttttaaggtt gtacgaaccc ctgccattgc tggcatgctt
    29761 aatcaacggg tatggcgtta atatggctgg tggattatcc agccggtatt tcgttattca
    29821 ggtacagcga tacttttttt aacgggaggc attcgccaga aattttttgc tcgtctcttg
    29881 cccggaggca tgattcttta ctggcataaa ttccggtaat cacattctgt ggctcacccg
    29941 ttataagaaa aacagtcatt accagtgcaa atgcagaagt cactgctgcc ccccgaaaat
    30001 accaagttta agaagggcaa ttctggagag tatggaatta tcattgagaa gataaggctc
    30061 atattttctc atcttaatgg catcttcagt aaactcccgg ttactgagca gaacaccaat
    30121 atcaaaacac ccttcagacg tattaacgtt tggtaataac gtttccatta tcgtgtcctc
    30181 aacaatgaat tttgtgatgt ggtgcctggt gcctccaggt gacgttaacc agttaacaat
    30241 taacgccgga atacagaagg atgcccgtta cgccccgtaa aagaccactt tacggattta
    30301 actgttccgc gtgcgcatag ccgcattcac cgcatcacaa aattcacttt aaaaaggatg
    30361 gcagagcagt catggagtcg aactgatgcc accaaagact acacatggat attgtggcgg
    30421 ggctgtcact taagcgtatg gtcagcctga caacccggtg tcctcaaggg gaaggaataa
    30481 ccccgccata cttaccgccg cgccatttcg cggattgcca caaccggaag cgcacggtcg
    30541 aattaaattt aacgacaaag tacagagaga cgagcttctc cgtgcgcttt cgtgttatgc
    30601 cctgactttt cagggtaaat taaccagtgg caacctgttt ttactggtgc taatcagtta
    30661 gtgtttctgg ctaaccagcg acgcgcgcca gcttcggttt taaacttttt acttttggta
    30721 tacgtcatcg cggtaaacgt gccgtcctgg ttagggaaca caccgcatac cagagattcg
    30781 ttgttgccaa gattgagcgt atccatgttg acctcatttc cccttaacgc cggggtagcg
    30841 gaacaaaaaa cctgctgcat agttaaacgt aatccccgcc gtcatgttca tacgcctcag
    30901 gctggctact taacccctga ccactgccgg gtaactcgaa gtattgccct gcgttctgtg
    30961 ggtcggggtg ggtgtatgga tacaatttac aaattaaaat ttaactggtc aacatgttta
    31021 ttattaaatt tgtaattgcg agctgttgca ttagtcccaa aatgggacta ctatacagtt
    31081 atgaagatta tctcagttaa aacactcagg gatttttggg cggagaatcc tgatgcagaa
    31141 caaccgctaa gggcatgggt ggatgaggcg gcaaaagctg actggaaaag cccagcagac
    31201 attaaggcac aataccgaac ggccagtata ttgaaaaacc ggcgcgtggt attcaacgta
    31261 aaaggtaatc attaccgttt aattgttgca attgcgtatc agcgaggatg ggtatttgtt
    31321 aaattcatcg gaaaccacaa ggaatacgat gctattgatg ctgataccat cgaactggag
    31381 taagcatgaa catcaaacct attcgtacag agcaagatta tgaagccgcg ttgcgtgctg
    31441 ttgaacccat gttcgacaat gagcccgaaa ttgacacgcc tgagggggat ttttttgagg
    31501 tgatgtgttt gctaatagag gaatatgaaa aaaaacatta tcccattgag ccaccatccc
    31561 caattgaagc tataagattt cgcatggaac agcaggggct gactgtgaaa gatttggaac
    31621 ccgcaattgg gaaaaaaaat cgggtttatg aggtgttgaa tggtaccaga agccttacgt
    31681 taccaatgat tcgccgtctt cataatcaat ttggtatccc cctggaaagc ctggttggat
    31741 tataaaatct gctagtcatt tgcctgatgc tcgttccaga aaaggaatgc atcaggcagt
    31801 tttgtttttc tgccgcagta actcttcaag tttccgttta tagaaatcgc gtttttgctc
    31861 catatcacga atgatctgct ctgcgtcgct ttgaggtaac tcatctaaaa gcgatatgat
    31921 ttttcgttgt tgttctgtaa gttgcggttg gttgtcatta ctggatacag ccattttatc
    31981 gccgagagtt tcttcttcca taaagaacca atggacggga tgttgtgaga gctctgctaa
    32041 tttttccagt ttatccattc ttggcatcac gcctttcaac caaccttgca cggattgggg
    32101 ttttacacca agacgtcttc ccagctctga ctggtttata ttcaattcct gcaacacctg
    32161 ctgaaggcgt tttacaaaga tcatcaccac ccctcgtaaa ctagttccgc gatcctacag
    32221 aaaaaattga taagtggcat tacaaataga agttgaaatt taaatttaaa tttgtaatca
    32281 tcggtgtcat cgtaaagttc ggagggaaac atgcaaaaaa gtactcaagt gaaaatcctg
    32341 tcaataatga gccaatcaga attagggcgt cgtcttggta aaacaccgca aaccataagt
    32401 gggtggttta aaaaacgagt gccagcggag gaggttatac cagcatgtga ggcgcttgac
    32461 tggggagtaa ctccgcatga attgcgccct gataaatacc ctaatccaac cgatggttta
    32521 cctgttgagt atcaggctaa cgcacaagca gcggcgggag ttgattcatg aaaatcaagc
    32581 atgagcacat ccgcatggcg atgaatgcgt ggctgcttta tccgagggta gggcgcaaaa
    32641 aaatcgctga tgatatagcg acagcatatt ttgagcttga aatgacttat ccaccaatgc
    32701 atgacacctc tacgacagag ggtattggat tgaatataca aaatattttt cgctggcttg
    32761 aaaaggatac gcctgatgct gttgaaaaaa ttcaggcact aattccggct atcctgactg
    32821 ttcttccgcg tgaactgcgt tatcacctca gtatttttga cactgttgag cgccgtgcat
    32881 tactggcggc gcaggaagcg ttgagcacgg caattgatgc acatgatgat gctgtccagg
    32941 ccgtttaccg gaaagcgtat ttcagcgacg gcgggtcatc cggcgagtct gttgtggtgc
    33001 attgatattt atgccagccc cctgctgatt ctgttgattg gggaatcaca gaatatcacc
    33061 agaggatggt tcgtcacaag atgaggcaat tatggccgca ttaccataca tgcaactgta
    33121 catagctgat tacctggctg acaccatgca tttgtccgca gaggagcacg gtgcgtattt
    33181 gttgctgatg ttcaattact ggcaaacagg gaaaccaata cctaaaaaca ggctggcaaa
    33241 aatttcccgt ctgactaacg agcgatgggc tgatgtggaa ccatccttgc gggagttttt
    33301 ttgcgataac ggcgatgaat gggtgcatct tcggattgag gaagatctgg catcagtcag
    33361 ggaaaaattg accaaaaaat cagccgccgg aaaagcatct gttcaggcca gaagaagcag
    33421 aaaggaagta tatgttcaaa caaaacaaga gagagattta acaggtgttc aaacaaatgt
    33481 tggtgttgtg tttgaacatg acgcaaacac aaaagcaact aataaagata cagatctaaa
    33541 agaattaaac cccacacata acgtgcgtga acgcgagagt attccgacca gtgagttgca
    33601 tggtgcgccg ttgcagacag ccgaacctga atacctggac ggcctgagcg aaccgatcgg
    33661 gaaattttcg atgaccactg tctggcagcc gtcgccggat tttcgacaac gggcagcagt
    33721 gtggggtatg gctctgcctg agccggaatt tacacctgct gagcttgccg cattccggga
    33781 ttactggatg gcggagggga aggttttcac acaggttcag tgggagcaga aatttgcccg
    33841 ccacgtgcag cacgtcaggg cacaggtaaa accagtcagc aagggggtaa gccatgcagc
    33901 atcaggtggc acggcatcac gggcaattca ggaaatccgg gcagcacgcg aacagtggga
    33961 acgagacaac ggatttatca gcaacggaaa cggcctggaa gctgtgggag cttatggggg
    34021 aggtgtattc gaaccgctgg actcagaaga acggggccgc accttcgaag ctctggattg
    34081 cccagattgg cgcgatgact gaacagcaaa tccggctggt ctgccgtcag tgcatggacc
    34141 gctgccgggc gggtgaaacg tggcccccgg acctggctga gtttgttgcg ctgatttcgg
    34201 ggagtggggc aaatccattt ggtcttacgg tggatgccgt gatggaagag taccggcgct
    34261 ggcgcaatga atcctggcga tacgacggga gtgataaata cccgtggcca cagcctgtgc
    34321 tgtaccacat ctgcctcgaa atgcgtacca gagggattga gcgccagatg acgcagggtg
    34381 agttaaaacg acttgcggaa cggcaactga cgaaatgggc aaagcatgtt ggtaacggga
    34441 tgagtgttcc gccagtgcga cgacaactgg aaggggcgaa acacccgcaa gggccaacgc
    34501 caattgaacg gctgaaacag gaatacgaac gccggaaggc agctggtttt atttgaatct
    34561 gagaaacgat tttgtcggag gaaattttaa tggaaaccgt atttgacgca ctgaaagcac
    34621 tgaaaaaagc ctcttcacag gtagtggcat cgcgccttgg aatcagccgc gaagatgcgg
    34681 tcaacgaact gtggaaactg aagcgccgcg gtgaagcgga taacaagggt tcgatgtggt
    34741 ggctgattca ggctggtgaa agtgaaccag tgtcaccggt accgaaagtg acagcgcaaa
    34801 tgctgattaa ggcgattgaa caacatggcc cacaaacggc ggatgagctg gcactgatgt
    34861 tcgggattac ctcccgccgg gcgaattcat cgctggccat ggcaatcagc aaagggcgtc
    34921 tgattcgcgt gaatcagggc ggtaaatttc gttactgcat accggtcgct gatttaccgg
    34981 cagagccgga agctgcatcc gtagcggaaa cggatggtaa agcctttcct cagccagcag
    35041 gtgttgcgtt accagtccgg gaagcggaaa cacaggaaga tattaaaaca gaaactgtgg
    35101 ctgatattgt gcaatcgttg ccatcgttta ctgaaacgcg agcggatgac ctggttttac
    35161 catcgctgca tgtggctaac cgcgagctgc gccgggcaaa aggtcaggtt cagaagtggg
    35221 agcgagtctg tgccgcgctg cgggaactga acaaacacag ggatattctc cgggatatta
    35281 ccgccaccag agagctgctg cggtgagtgg ctggaagaag tggcgctggg cggaaattat
    35341 gatactccgg cagtgtgcgg gaacgatgac agtcgaaagc atcggcagtc tgattggccg
    35401 tagtggggct gccgtcagga cgaaagcgcg ggaactgggt atctgcatga tgttacgggg
    35461 tgattatcac cagtcagcca aatgttcaca gcgtgatatt gagctggcgc ggcaactgca
    35521 tcagcgtggc gtaccccgac gggaaattgc cgaaaagttt gggatgaagt tgggcgcagt
    35581 gaataactac gtttatttcg acaggagggt tcaggcgtga gggtgagaat ttatatcgcc
    35641 ggtccgatga cgggatatga aaatttcaac cgtgaggcgt ttcacaaggc ggaagaagca
    35701 ctgaaacagg aagggcatac cgtattaaat cccgcagtac ttccggacgg gctgactcag
    35761 ccacattaca tggatatttg tatggcaatg attcgttgtg tggatgcggt ttatatgctg
    35821 aaaggctggc agcggtcggc aggcgctaag gcagaactgg cactggcgga gaaactgggg
    35881 catgcagtta ttttccagga ggcaaccagt gagaaaaatt aattatcaga cgctacgtga
    35941 gatagcaaaa ctggcaaccc agggcgaatg ggtcgcattc atttcgccgg gcactgatac
    36001 atatgcggtg catacgccgg gggatgaacg ttgcggagac attatcaaat ggcctggctt
    36061 tgatgaccag aaaaatgcag agaacaacgc agaatttatt gcagctttca accctgaagt
    36121 agtacaggca ctgctggatg aacgggaagc ccaaagcaaa cgtattgccg agctggaagc
    36181 taatcttgtg gcgctggctg cggagaatgc acttgctcgt aaagcagttc aggcattctg
    36241 cgatgttgtt ggcgacaaca ccgaggttat ttgcgaggag gttgggagag atggcgttct
    36301 ggttattttg gaggccatga aggcaacagg aaatatgcca gccaccgatg ctttcctggc
    36361 tgatgtacga gcggaggtgt ttaacgacct ttgcgcggcg tttgtcaggc acgcaaaagt
    36421 tgcaggactg gatgatgccg ataccgtaac gtttaaggaa gcgacagaag cattgctgca
    36481 ttgtgcagaa caacttcgcg cacctgaata attaaattta gcactgtaaa taaaatttaa
    36541 tccttaaccg gagggattcc tgcaccctca gaacatcagg agaccgcccg gcagggcggt
    36601 agtgaaatgc gaaagttcaa aataattatt gaaacgggaa tagccggtgg agattttgag
    36661 gatgtattcg aagtggacga tgacgcaaca cctgatgaaa ttcatgacga agcaaaagaa
    36721 attttcttta actactgcaa ttactcatat cacgaaataa aagacgaaga ggaagaacaa
    36781 aatggctgat tttggttcaa ctaaatataa cgccagtttt gaagaatggc atgaactgtt
    36841 aatggactat gcagagttac gcggtggaag tgccgccgat gctgaagcat ggcgtgatga
    36901 ctatgaagca ggaaaaactc cggttgaagc ttattgtgat gagtggggtg aggacgatga
    36961 agattaacta tcaggcgatg gtgcaggcca ggcgattcga taccggctcg tgttcagagt
    37021 catgtgaaat ctggggtgaa caattggaat tgaaattcga agaggtggtg gcatgacaat
    37081 cgtaaaaacc cataccggca tcgtgatcac caaagacggt ccgcaggtaa aaaaactgca
    37141 ccagacggag cggatgtggg tcgtcgacaa aaacgagttt taccacaaag aaaccggacg
    37201 ccgccacttt gcagaaaata cgcgccgccg actactgatc gacaccatca agcctgtcga
    37261 ggtgaagcat gtttaaacag aacgaaaaat ctatcgctca aattgctgag tatatcccgc
    37321 gtgcgtgccg gggtatgcag ttgcaggaag ccaaagcgcg cctggagaaa aaaattgcgc
    37381 tctatatcaa tgacggctgt gatgctgccg ttcttaacgc ggcgttcgca ccagctctta
    37441 acagtcatac gcgggagtct tttttttcgt gcatcgcagc gcagatccac aaaggaggca
    37501 accagtgagc aagattgact atcaggcatt gcgtgaggca gcacaaaact atcggtcgat
    37561 gctggcgtgg tatcaggaga aaccagacag tccaaacgct gagcaggatt gtgatgcagc
    37621 tttgtctgcg tttaagcgag aaatccgtca tcgtgaagtg gatattatcg ctgatttgct
    37681 ggatgaactg gaggaagcaa aacagcgtat cgacgagcag gagtcccgca cagtgaagtt
    37741 accagaacca ttcaagctgg ctaaatcatc aagtggatta acgtactact acgctgatga
    37801 ggtcaatgca gcactgactg ctgctggtat tcgcatagaa ggagagtgag atgaccacta
    37861 ttaccaaaga gcgactgcaa tggctggcta acatttctgg ccgcgatgac attgacgata
    37921 tagacggcgg tgaaattcgt gagctggcgc ttatcgcact ggcatcgctg gaagcagaac
    37981 cggtggttta catgtgggat agcgaaagaa aagatattga tgctcctggg tattatcgga
    38041 ctgagcattt agtgttcgct gagagtagtg ttaagcaatg gggagggcga gttgttccac
    38101 tttacaccgc cccgccagcg ccgatagtgc cagctgttct ggaacgtttg cgagctattg
    38161 tagcggaccc acgcgcatta cctcgcagaa aagaatgggt tagcgggcag cagtacagtt
    38221 acgtacttct cgaaaacgta gaagctatgg ttgatgaagc ctgccgcgca gccatgcttc
    38281 agggagataa ataatgatta atcgaaccaa actggagcac attctcgaat atgccaggca
    38341 gcagaaatgt attgggcaac tttgtaaaat tccaccaggg gatatggttg aaatcatgga
    38401 gattgccatg cgcaaggctg gcaactctcc gataactcag gctagtctgc ctggcggttt
    38461 caccattgag gaggcgaagg agttacatga agacctggtg cgcagccaca taagcaaggc
    38521 tttaagtggc gaaaagatga agaagaagga tctcgatgct gatttgtgct ggatccatgg
    38581 ggttatagtc caggcatcct ggtttgtaaa agcatcactg gagcagaatg cgctaacggg
    38641 taactctcag gtaatccctg atgacggtcg cgaacagttt gaaacactta tcaggttcca
    38701 tgcagatgat aaaaaccatg aaacattatt gcttcgtgct aacgaaggga tgaattaccg
    38761 ggatccaaat gtggatttgg cgtggatatt ctggaaatcc agtcgagagc acatattgcc
    38821 gggaaaaagc gacaataatc cagcattagg cgatcaggtg agtgaattaa ctatgttggt
    38881 caagcgatta gccagttcat taaaaagcgt caataaatca agcaaactac cggataaagc
    38941 gatggactac ctgaagcgaa acggactgat aagcgcggag gatattttac gatgacctgg
    39001 cctgaggcat tcgtggcggt aggaattgca atggcggtgg cgctggtgct ggtggtgtat
    39061 tcgatttgcc gctggggata aaaacggttt gcggggaaag aagagttaag tagaagtgct
    39121 gcgggtgctt gaggctgtct gcctcgggca tgccgccgta aggcagacag agaaaagccc
    39181 cagttaacat tacgcgtctt gcaggacgct taacattaat ctgaggccca atctatgctt
    39241 cacaaacgta ggttagcctc ttacggaccg aaaggtcaag gagaagcaga ctatgaagca
    39301 gcaaaaggcg atgttaatcg ccctgatcgt catctgttta accgtcatag tgacggcact
    39361 ggtaacgagg aaagacctct gcgaggtacg aatccgaacc ggccagacgg aggttgctgt
    39421 cttcgtagac tacgaatctg agaagtaaga gtgaccaggc gagggagaaa tccctcgcca
    39481 cctctgatgt gtcaggcatc ctcaacgcac ctgagataaa atctatttgg ctgtttctgg
    39541 gttcgaactg atgattattt ggtgctaata catgatttaa ttccctgaat cctcttgctt
    39601 ttcccattgg tggggtctaa gactcctcga agggaataaa aatcaatgat agtagaacca
    39661 tctgccaact tataaatgtc ggcattatct ccaaccgcta atacgctgta atacttgcca
    39721 attttttgag acgtgatagt ggctaagaga ggtgtacgct gccactcact gagaatgcat
    39781 tcttgtattt cttgtggtgg agaatttgtt tt
//
LOCUS       DAZKGF010000021        39218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZKGF010000021 DAZKGF010000000
VERSION     DAZKGF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.021"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..220
                     /locus_tag="ACHOQV_001111"
     CDS             <1..220
                     /locus_tag="ACHOQV_001111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629522.1"
                     /translation="LIMVCDFKFVLWINCEAELIREEKQKKTPQSAAFGIVCIFLAGC
                     GVNASPGLVELKTTRLLSPRYHAALRCG"
     gene            complement(171..1835)
                     /gene="glnS"
                     /locus_tag="ACHOQV_001112"
     CDS             complement(171..1835)
                     /gene="glnS"
                     /locus_tag="ACHOQV_001112"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFP9629523.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGSFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSCEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2038..3984)
                     /gene="nagE"
                     /locus_tag="ACHOQV_001113"
     CDS             complement(2038..3984)
                     /gene="nagE"
                     /locus_tag="ACHOQV_001113"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFP9629524.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDVMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4317..5117
                     /gene="nagB"
                     /locus_tag="ACHOQV_001114"
     CDS             4317..5117
                     /gene="nagB"
                     /locus_tag="ACHOQV_001114"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9629525.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5177..6325
                     /gene="nagA"
                     /locus_tag="ACHOQV_001115"
     CDS             5177..6325
                     /gene="nagA"
                     /locus_tag="ACHOQV_001115"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9629526.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            6334..7554
                     /gene="nagC"
                     /locus_tag="ACHOQV_001116"
                     /gene_synonym="nagR"
     CDS             6334..7554
                     /gene="nagC"
                     /locus_tag="ACHOQV_001116"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFP9629527.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7602..8354
                     /gene="nagD"
                     /locus_tag="ACHOQV_001117"
     CDS             7602..8354
                     /gene="nagD"
                     /locus_tag="ACHOQV_001117"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFP9629528.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8610..10274
                     /gene="asnB"
                     /locus_tag="ACHOQV_001118"
     CDS             8610..10274
                     /gene="asnB"
                     /locus_tag="ACHOQV_001118"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFP9629529.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10655..10731
                     /locus_tag="ACHOQV_001119"
     tRNA            10655..10731
                     /locus_tag="ACHOQV_001119"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10689..10691,aa:Met,seq:cat)
     gene            10740..10824
                     /locus_tag="ACHOQV_001120"
     tRNA            10740..10824
                     /locus_tag="ACHOQV_001120"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10774..10776,aa:Leu,seq:tag)
     gene            10848..10922
                     /locus_tag="ACHOQV_001121"
     tRNA            10848..10922
                     /locus_tag="ACHOQV_001121"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10880..10882,aa:Gln,seq:ttg)
     gene            10957..11031
                     /locus_tag="ACHOQV_001122"
     tRNA            10957..11031
                     /locus_tag="ACHOQV_001122"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10989..10991,aa:Gln,seq:ttg)
     gene            11047..11123
                     /locus_tag="ACHOQV_001123"
     tRNA            11047..11123
                     /locus_tag="ACHOQV_001123"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11081..11083,aa:Met,seq:cat)
     gene            11172..11246
                     /locus_tag="ACHOQV_001124"
     tRNA            11172..11246
                     /locus_tag="ACHOQV_001124"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11204..11206,aa:Gln,seq:ctg)
     gene            11284..11358
                     /locus_tag="ACHOQV_001125"
     tRNA            11284..11358
                     /locus_tag="ACHOQV_001125"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11316..11318,aa:Gln,seq:ctg)
     gene            complement(11512..12687)
                     /gene="ubiF"
                     /locus_tag="ACHOQV_001126"
     CDS             complement(11512..12687)
                     /gene="ubiF"
                     /locus_tag="ACHOQV_001126"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFP9629530.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVMVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            12833..14257
                     /gene="miaB"
                     /locus_tag="ACHOQV_001127"
     CDS             12833..14257
                     /gene="miaB"
                     /locus_tag="ACHOQV_001127"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFP9629531.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14410..15450
                     /gene="ybeZ"
                     /locus_tag="ACHOQV_001128"
     CDS             14410..15450
                     /gene="ybeZ"
                     /locus_tag="ACHOQV_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP9629532.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15447..15914
                     /gene="ybeY"
                     /locus_tag="ACHOQV_001129"
     CDS             15447..15914
                     /gene="ybeY"
                     /locus_tag="ACHOQV_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP9629533.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            16004..16882
                     /gene="corC"
                     /locus_tag="ACHOQV_001130"
     CDS             16004..16882
                     /gene="corC"
                     /locus_tag="ACHOQV_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFP9629534.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16907..18445
                     /gene="lnt"
                     /locus_tag="ACHOQV_001131"
     CDS             16907..18445
                     /gene="lnt"
                     /locus_tag="ACHOQV_001131"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFP9629535.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKH"
     gene            complement(18523..20400)
                     /locus_tag="ACHOQV_001132"
     CDS             complement(18523..20400)
                     /locus_tag="ACHOQV_001132"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP9629536.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLLSSVFLHSSVSHLLMNMFALLVVGGAVERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLSSGAGTHHKNQR
                     RVFSLLGMVALTLLYGARQAGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWFVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSLDEKLIYATSFEANAVSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVTDIPLEKYDGTGTSGKPGAWV
                     MALSPDEKTLLIPGMVRGDIVRINTITHQKEDFPTGDARGIVSAMGFRPKNGEIIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNRLIQMDRTRSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            20855..21763
                     /gene="gltI"
                     /locus_tag="ACHOQV_001133"
     CDS             20855..21763
                     /gene="gltI"
                     /locus_tag="ACHOQV_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFP9629537.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21933..22673
                     /gene="gltJ"
                     /locus_tag="ACHOQV_001134"
     CDS             21933..22673
                     /gene="gltJ"
                     /locus_tag="ACHOQV_001134"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFP9629538.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            22673..23347
                     /gene="gltK"
                     /locus_tag="ACHOQV_001135"
     CDS             22673..23347
                     /gene="gltK"
                     /locus_tag="ACHOQV_001135"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFP9629539.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23347..24072
                     /gene="gltL"
                     /locus_tag="ACHOQV_001136"
     CDS             23347..24072
                     /gene="gltL"
                     /locus_tag="ACHOQV_001136"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFP9629540.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24190..25125
                     /gene="rihA"
                     /locus_tag="ACHOQV_001137"
     CDS             24190..25125
                     /gene="rihA"
                     /locus_tag="ACHOQV_001137"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFP9629541.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRNLIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLCESEEPITIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(25170..25652)
                     /gene="ybeL"
                     /locus_tag="ACHOQV_001138"
     CDS             complement(25170..25652)
                     /gene="ybeL"
                     /locus_tag="ACHOQV_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFP9629542.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25887..28469
                     /gene="leuS"
                     /locus_tag="ACHOQV_001139"
     CDS             25887..28469
                     /gene="leuS"
                     /locus_tag="ACHOQV_001139"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP9629543.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            28484..29065
                     /gene="lptE"
                     /locus_tag="ACHOQV_001140"
     CDS             28484..29065
                     /gene="lptE"
                     /locus_tag="ACHOQV_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFP9629544.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            29065..30096
                     /gene="holA"
                     /locus_tag="ACHOQV_001141"
     CDS             29065..30096
                     /gene="holA"
                     /locus_tag="ACHOQV_001141"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP9629545.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAIRQVAAV
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHMPLRALFDKH
                     RVWQNRRGMMGEALNRLSHPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            30098..30739
                     /gene="nadD"
                     /locus_tag="ACHOQV_001142"
     CDS             30098..30739
                     /gene="nadD"
                     /locus_tag="ACHOQV_001142"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP9629546.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30763..31374
                     /gene="cobC"
                     /locus_tag="ACHOQV_001143"
     CDS             30763..31374
                     /gene="cobC"
                     /locus_tag="ACHOQV_001143"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFP9629547.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            31634..31951
                     /gene="rsfS"
                     /locus_tag="ACHOQV_001144"
     CDS             31634..31951
                     /gene="rsfS"
                     /locus_tag="ACHOQV_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP9629548.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31955..32422
                     /gene="rlmH"
                     /locus_tag="ACHOQV_001145"
     CDS             31955..32422
                     /gene="rlmH"
                     /locus_tag="ACHOQV_001145"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP9629549.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            32453..34354
                     /gene="mrdA"
                     /locus_tag="ACHOQV_001146"
     CDS             32453..34354
                     /gene="mrdA"
                     /locus_tag="ACHOQV_001146"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFP9629550.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            34357..35469
                     /gene="mrdB"
                     /locus_tag="ACHOQV_001147"
                     /gene_synonym="rodA"
     CDS             34357..35469
                     /gene="mrdB"
                     /locus_tag="ACHOQV_001147"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFP9629551.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            35480..36568
                     /gene="rlpA"
                     /locus_tag="ACHOQV_001148"
     CDS             35480..36568
                     /gene="rlpA"
                     /locus_tag="ACHOQV_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFP9629552.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGLYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36708..37919
                     /gene="dacA"
                     /locus_tag="ACHOQV_001149"
     CDS             36708..37919
                     /gene="dacA"
                     /locus_tag="ACHOQV_001149"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFP9629553.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            38029..38292
                     /gene="ybeD"
                     /locus_tag="ACHOQV_001150"
     CDS             38029..38292
                     /gene="ybeD"
                     /locus_tag="ACHOQV_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFP9629554.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            38393..39034
                     /gene="lipB"
                     /locus_tag="ACHOQV_001151"
     CDS             38393..39034
                     /gene="lipB"
                     /locus_tag="ACHOQV_001151"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFP9629555.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         9610 a        10187 c        10064 g         9357 t
ORIGIN      
        1 tttaattatg gtttgtgatt ttaaatttgt tttatggata aattgcgaag cggaattaat
       61 tcgcgaagaa aagcagaaaa aaacgccgca gagcgcggcg tttgggattg tttgcatatt
      121 tcttgccgga tgtggcgtaa atgcctcacc cggcctggta gaacttaaaa ctactcgcct
      181 actttcgccc aggtatcacg cagccctacg gtgcggttaa ataccggttt ttcggcagtg
      241 gaatggcggc tgtcgaggca gaaataacct tcacgctcaa actggaatgc tttacccgct
      301 accgcgtctt tcagcgacgg ttcagcgaaa ccctgtttga tcaccagcga ttccgggtta
      361 atcaccgaca ggaaatcatc cgcagcacct gggttaggca cgctgaacag acggtcatac
      421 aaacggattt caaccggcag cgcatgtgcc gcgctcaccc agtgaataac acctttcact
      481 ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt cataagtaca gaagatggtg
      541 gtgatattac cttcggcatc tttctcgacg cgttctgcct taatcacgta agcattacgc
      601 agacgcactt ctttacccaa caccagacgt ttgtactgct tgttagcttc ttcgcggaaa
      661 tcggcgcgat cgatccaaat ctcacagcta aacggcacct gacggctacc catttccggt
      721 ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc cctggtagtt ttcgataacc
      781 agtttcaccg gatcgataac cgccattgcg cgcggggcat tttcgttgag atcttcacgg
      841 atgcaggatt ccagtgacgc catctcaatg gtgttgtcct gcttggtcac accaatgcgt
      901 ttgcagaact cacgaataga agccgcagtg taaccacgac gacgcagacc agaaatggtc
      961 ggcatacgcg ggtcatccca gccttcaacg tgcttatcgg tcaccagcag gttcagctta
     1021 cgcttggaca tcacagtgta ttccagattc aggcgcgaga attcgtactg gcgcgggtga
     1081 acaggaatgg tgatgttgtc cagtacccag tcgtacagac ggcggttgtc ctggaactca
     1141 agcgtacaca gagagtgcgt aataccttcc agcgcatcgc tgatgcagtg ggtgaagtcg
     1201 tacatcgggt agatgcacca cttgttgcca gtctggtggt gttcagcaaa tttaatgcgg
     1261 tacagcaccg gatcgcgcat tacgataaat ggcgacgcca tgtcgatttt cgcacgcagg
     1321 caggctttac cttcttcaaa actaccggta cgcatttttt cgaacagcgc caggttctct
     1381 tcaacgctgc ggtcgcggta cgggctgttt ttacccggct gcgtcagggt gccgcggtat
     1441 tcgcggatct gttccggtgt cagttcatca acgtacgcca ggcctttatt gatcagttcg
     1501 atcgcatagg cgtggagctg atcaaaataa tcggaggagt aacggacgtt accagaccag
     1561 tgaaaaccta accactcaac gtcgtttttg atcgactcaa catactcgat atcttctttt
     1621 accgggttag tgtcgtcgaa acgcaggttg cactggcctt tatagtcctg ggcgatcccg
     1681 aagttcaggc agatagattt cgcatggcca atatgcagat agccattcgg ctccggcggg
     1741 aaacgggtgt gtactgtggt gtgcttacca ctggccagat cttcatcgat gatctgacga
     1801 ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg attcctcaaa gcgtaaacaa
     1861 cgtataacgg cgtatgatct tataagcggg acaggctgac aactgttagt tagtgaaaat
     1921 cgaaaatctc actgagatta tggggatgat tgatgaaaaa aaaagcggca gagaagatct
     1981 ccaccgcttg tcattgttgg atgcgacgct caagcgtcgc atcaggcata aagcagatta
     2041 ctttttgatt tcatacagcg gtgtttgacc cgccacaaca tggccctgag ctttaatgat
     2101 caggccgctg aaatcgtcga tattgctgca aaccaccggg ctaatcatcg agcgggcgtt
     2161 agcgttcagg taatccagat ccatttccag aatcggttgt cctgcgctta cctgcgcgcc
     2221 ctcttccacc agacgtttaa agcctttacc ttccagcgct acggtgtcga tacccatatg
     2281 gacgacgatc tccgcgcctt tttcggtttc cagacagaac gcgtggttgg tgttgaagat
     2341 tttcacgatt gtccctgcgg ctggtgatac gacgatttta tctgtcggtt tcaccgccac
     2401 accgtcaccc accgctttgc tggcgaatgc ttcgtcagga acctgatcca gtgccacaac
     2461 atcaccggta atcggcgata ccagctccgc gatagacacc gcgtttggta cagcctgcgg
     2521 ttttgctaca ggcgcggcag ttgcaggcgc agtttcagct gacgcagcgg ctaccggacc
     2581 acgggcaacg actttcttca tcgcatcgcc gatggattct gctttcgcgc ccacaatcac
     2641 ctgaatagtc tgtttgttca gttttactac cccagaagcc cccagacgtt tacacatcac
     2701 atcgttgacg cgagctgagt caaccacggt aaggcgcaga cgagtaatac aggcgtcaat
     2761 ggctttcagg ttgtcagtac cgccaaccgc agcaatatag ttggttgcca gttgattgag
     2821 accttcttcc gtgttgctgt tggcttcttc agtaacgatc tcgtcttctt tatcttcacg
     2881 gcccggcgtt ttcaggttga acatgcggat aaccaaactg aacaccacga agtagatagc
     2941 gaagaagaca acacccatca ccagcagcat ccagacgttc tggctggcgg ccggcaggtt
     3001 atacatcaac gcgtagtcga tagcccccgc agagaaggag aagcccgcgt ggatacccag
     3061 cagcgttgcc acaaacaggc tgataccggt cagcagtgcg tgcaggaggt acagcagcgg
     3121 cgcaaggaac atgaacagga attccagcgg ctcagtcaca ccggtcagga acgcagtaac
     3181 agcaacagaa agcagcatac cgccaaccat cggacgacgc tctttcggtg ctgcaaagta
     3241 catcgccagc gccgcacccg gcagaccgaa catcatgatc gggaagaagc cggacatgaa
     3301 catccccgcg gtgccgtcac cggcatagaa gcggttaatg tcgccgtgga aaaccgtacc
     3361 cgccgcgttg gtgaattcac caatctggaa ccaggcgatg gtgttcagta cctgatgcag
     3421 accggttggg atcagcagac ggttgatgaa accaaagata ccggaaccca gcgcgcccgc
     3481 agaaacgatc cactcgccgc ctgcatggat agcgtgctgt accggcggcc agacgtaacc
     3541 aaaaatggcc gccagcacca ggcagaagaa gccggtggca atcggcacaa agcgtttgcc
     3601 gccgaagaag ctcaggaagt ccggcagttt aatatcggac caacggttat aggctgcacc
     3661 accgaccaga ccggtaatga tacccgccag tacacccatg ttaatttctg ggttgatggt
     3721 caccatcgct ttggttaaca caaagtaacc taccgcaccc gccagtgccg ccgcacctgc
     3781 gctgtctttc gaccagctgg atgccacgcc gatggcgaag attaatgcga ggttatcaaa
     3841 aatcgcaccg cccgcctggg caataaacgc aacgttaagt aaatctggct gaccgaatcg
     3901 cagcaacagc gccgccaccg gcagcaccgc gatagggagc tgtaacgccc tacctagtcg
     3961 ctggaaaaaa cctaaaatat tcatcttatt ccccctacga gaaccctatt tggctcgttt
     4021 aaagccgtat ttttattttg ctgcaaattg tactgccgat gttctgtaat cagattgtta
     4081 gatcatctgc tacagagtgt gtgaaaattt aattcgtatc gcaaattaaa cgcgtgtctt
     4141 ttgtgagttt tgtcaccaaa tatcgttatc atcactccct tttactggct aaaccagaaa
     4201 acttatttta tcattcaaaa aatcaggtcg gattgacgcc tgtctgcgca aatccaggtt
     4261 acgcttaaag atgcctaatc cgccaacggc ttacatttta cttattgagg tgaataatga
     4321 gactgatccc cctgactacc gctgaacagg tcggcaaatg ggctgctcgc catatcgtca
     4381 atcgtatcaa tgcgttcaaa ccgactgccg atcgtccgtt tgtactgggc ctgccgactg
     4441 gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat gcataaagca ggccaggtca
     4501 gctttaagca cgttgtcacc ttcaacatgg acgaatatgt cggtctgccg aaagagcatc
     4561 cggaaagcta ctacagcttt atgcaccgta atttcttcga tcacgttgat attccagcag
     4621 aaaatatcaa ccttctcaac ggcaacgccc cggatatcga cgccgaatgc cgccagtatg
     4681 aagaaaaaat ccgttcttac ggaaaaattc atctgtttat gggcggtgta ggtaacgacg
     4741 gtcatattgc atttaacgaa ccggcgtctt ctctggcttc tcgtactcgt atcaaaaccc
     4801 tgactcatga cactcgcgtc gcgaactctc gtttctttga taacgatgtt aatcaggtgc
     4861 caaaatatgc cctgactgtc ggtgttggta cattgctgga tgccgaagaa gtgatgattc
     4921 tggtgctggg tagccagaaa gcactggcac tgcaggccgc cgttgaaggt tgcgtgaacc
     4981 acatgtggac catcagctgt ctgcaactgc atccaaaagc gatcatggtg tgcgatgaac
     5041 cttccaccat ggagctgaaa gttaagactt taagatattt caatgaatta gaagcagaaa
     5101 atatcaaagg tctgtaatta ttatccctgc cctggctcct tgctcagggc aatatttttt
     5161 aaaatcgggg gtcagaatgt atgcattaac ccagggccgg atctttaccg gccacgaatt
     5221 tcttgatgac cacgcggttg ttatcgctga tggcctgatt aaaagcgtct gtccggtagc
     5281 ggaactgccg ccagagatcg aacaacgttc actgaacggg gccattctct cccccggttt
     5341 tatcgatgtg cagttaaacg gctgcggcgg cgtgcagttt aacgatactg ctgaagcagt
     5401 cagcgtagaa acgctggaaa tcatgcagaa agccaatgag aaatcaggct gtactaacta
     5461 tttgccgacg cttatcacca ccagcgacga gctgatgaaa cagggcgtgc gcgttatgcg
     5521 cgagtacctg gcaaaacatc cgaatcaggc gttaggtctg catctggaag gtccgtggct
     5581 gaatctggta aaaaaaggca cccataatcc gaattttgtg cgtaagcctg atgccgcgct
     5641 ggtcgatttc ctgtgtgaga acgccgacgt gattaccaaa gtgactctgg caccggaaat
     5701 ggttccagcg gaagtcatca gcaaactggc aaatgccggg attgtggttt ctgctggtca
     5761 ctccaacgcg acgttgaaag aagcgaaagc cggtttccgc gcggggatta cctttgccac
     5821 ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa ccgggcctgg cgggcgcgat
     5881 cctcgacgaa gctgacattt attgcggtat tattgctgat ggcctgcatg ttgattacgc
     5941 caacattcgt aacgctaagc gtctgaaagg cgacaaattg tgtctggtta ccgatgccac
     6001 cgcgccagca ggtgccaaca ttgaacagtt catttttgcg ggtaaaacaa tatactaccg
     6061 taatggactt tgtgtggatg agaacggtac gttaagcggt tcatccttaa ccatgattga
     6121 aggcgtgcgt aatctggtcg aacattgtgg tatcgcactg gatgaagtgc tgcgtatggc
     6181 gacgctctat ccggcacgtg cgattggcgt tgagaaacgt ctcggcacgc tcgccgcagg
     6241 taaagtagcc aacctgactg cattcacacc tgattttaaa atcaccagga ccatcgttaa
     6301 cggtaacgag gtcgtaactc aataagagaa agtatgacac caggcggaca agctcagata
     6361 ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg tttatcgcct gattgaccag
     6421 tacgggccaa tctcgcggat tcagattgcc gagcaaagtc agcttgcccc cgccagcgta
     6481 accaaaatta cgcgtcagct tatcgaacgc gggctgatca aagaagttga tcagcaggcc
     6541 tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa cccgcaattt ccacgcaatc
     6601 ggcgtacggc ttggtcgtca cgacgccacc atcaccttgt ttgatctcag cagcaaagtg
     6661 ctggcagaag aacattaccc gctgccggaa cgtacccagc agacgctgga acatgccctg
     6721 ctgaatgcca ttgctcagtt tattgatagc taccagcgca agctacgcga gctgatcgcg
     6781 atttcggtga tcctgccagg gcttgtagac ccggacagcg gcaaaattca ttatatgccg
     6841 catattcagg tagaaaactg ggggctggtt gaagcactgg aagagcgttt taaagtgacc
     6901 tgttttgtcg gccacgatat ccgcagtctg gcgctggcag agcactactt cggtgcaagt
     6961 caggattgcg aagactccat tctggtgcgt gtccatcgcg gaaccggggc cgggattatc
     7021 tctaacgggc gaatttttat cgggcgcaac ggcaacgtcg gtgaaattgg ccatattcag
     7081 gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg gctgcctgga aactatcgcc
     7141 gccaatgctg ccattgaaca acgggtgttg aatctgttaa agcagggcta ccagagccgt
     7201 gtgccactgg acgactgcac catcaaaact atctgcaaag ccgcgaacaa aggcgatagc
     7261 ctggcctcgg aagtgattga atatgtcggt cgtcatctgg gtaaaaccat cgccattgct
     7321 atcaacttat ttaatccgca aaaaattgtt attgctggag aaatcaccga agccgataaa
     7381 gtgctgctcc ctgctattga aagctgcatt aatacccagg cgctgaaggc gtttcgtact
     7441 aatctaccgg tggtacgttc tgagttggat caccgctcag caatcggcgc ttttgcgctg
     7501 gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt tgctggaaaa ttaatgtgct
     7561 tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc catgaccatt aaaaatgtaa
     7621 tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc cgtaccgggt gcagcggaat
     7681 ttttgcacgg gattatggat aaaggcctgc cgctggtgtt gctgaccaac tatccttcgc
     7741 agaccgggca agatctggcg aatcgctttg ccactgccgg tgtcgatgta ccggatagcg
     7801 tgttttatac ctccgcgatg gcgaccgccg atttcctgcg tcgccaggaa ggcaagaaag
     7861 cgtatgtggt gggcgaaggc gcactgattc atgaactgta caaagccggt ttcactatta
     7921 ccgatgtgaa ccctgatttt gtgattgttg gcgaaacgcg ttcctacaac tgggacatga
     7981 tgcataaagc agcctatttc gtcgctaacg gcgcacgctt tatcgccacc aacccggaca
     8041 cccacgggcg cggtttttat cccgcctgtg gcgcgttgtg tgcagggatc gagaaaatct
     8101 ccgggcgcaa accgttctat gttggaaagc cgagtccgtg gatcatccgc gcggcattaa
     8161 acaaaatgca ggcgcattcg gaagaaacgg tgattgtcgg cgataacttg cgtaccgata
     8221 ttctggcggg cttccaggcc ggtctggaga cgattctggt gctttctggt gtttcgtcac
     8281 tcgacgatat cgacagtatg ccgttccgcc caagttggat ttacccatcg gtcgctgaaa
     8341 tcgacgttat ctgaatagca accacgcctc agggcgtggt ttcttatttt tacccatcat
     8401 taaaatcacc ccattcaata aaacccgtca aaaattatcg atccatcaat aaatataccc
     8461 aaaagccctc ccctttttca tcaatcagca atcgccatta cgttttttat ttttccactg
     8521 ccaaatcgct tgcgcaccgt gctgctttgc ggcatttttt taacaagtaa acacaacacg
     8581 cagtaaacac caggttaacg gagaaggtta tgtgttcaat ttttggcgta ttcgatatca
     8641 aaacagacgc agttgagctg cgtaagaaag cactcgagct gtcacgcctg atgcgtcatc
     8701 gtggcccgga ctggtccggt atttatgcca gcgataacgc catcctcgcc cacgaacgtc
     8761 tgtcaattgt tgacgttaac gcgggggcgc aacctctcta caaccaacaa aaaactcatg
     8821 tgctggcagt aaacggtgaa atctacaacc accaggcatt gcgcgctgaa tatggcgatc
     8881 gttaccagtt ccagaccggg tctgactgtg aagtgatcct cgcgctgtat caggaaaaag
     8941 ggccggaatt tctcgacgac ttgcagggca tgtttgcctt tgccctgtac gacagcgaaa
     9001 aagatgcata tctgattggt cgcgaccatc tggggatcat cccactgtat atgggctatg
     9061 acgaacacgg tcagctgtat gtggcctcag aaatgaaagc cctcgtgcca gtttgccgca
     9121 cgattaaaga gtttccggcg gggagctatt tgtggagtca ggacggcgaa atccgttctt
     9181 attatcatcg cgactggttc gactacgatg cggtgaaaga taacgtgacc gacaaaaacg
     9241 agctgcgtca ggcactggaa gattccgtta aaagccatct gatgtctgat gtgccttacg
     9301 gtgtgctgct ttctggtggt ctggattcct caattatttc cgctatcacc aagaaatacg
     9361 ccgcccgtcg cgtggaagat caggaacgct ctgaagcctg gtggccgcag ttgcactcct
     9421 ttgctgtagg tctgccgggc tcaccggatc tgaaagcagc ccaggaagtg gcaaaccatc
     9481 tgggcacggt gcatcacgaa attcacttca ctgtacagga aggtctggat gccatccgcg
     9541 acgtgattta ccacatcgaa acttatgatg tgaccactat tcgcgcttca acaccgatgt
     9601 atttaatgtc gcgtaagatc aaggcgatgg gcattaaaat ggtgctgtcc ggtgaaggtt
     9661 ctgacgaagt gtttggcggt tatctatact tccacaaagc gccgaacgct aaagagttgc
     9721 atgaagagac ggtgcgtaaa ctgctggccc tgcatatgta tgactgcgcg cgtgccaaca
     9781 aagcgatgtc agcctggggc gtggaagcac gcgttccgtt cctcgacaaa aaattccttg
     9841 atgtggcgat gcgcattaac ccacaggata aaatgtgcgg taacggcaaa atggaaaaac
     9901 acatcctgcg tgaatgtttt gaagcgtatc tgcctgcaag cgtggcctgg cggcaaaaag
     9961 agcagttctc cgatggcgtc ggttacagtt ggatcgacac cctgaaagaa gtggcagcgc
    10021 agcaggtgtc tgatcaacag ctggaaactg cccgcttccg cttcccgtac aacacgccga
    10081 cctcaaaaga agcgtatctg taccgggaga tctttgaaga attgttcccg cttccgagcg
    10141 ccgctgagtg cgtgccgggc ggtccttccg tcgcttgttc ttccgctaaa gcgattgagt
    10201 gggatgaagc gttcaagaaa atggacgatc cgtctggtcg cgcggttggc gttcaccagt
    10261 cggcatataa ataagacgtg taatattgcc ccggagaaat ccggggcaat ttcagttact
    10321 ggggcgcttg ttgattctga tatgcagtaa ggaaatgttc cgggtttgcc acatcgctgt
    10381 ttgacaactc attactgtag gccagtattt catagattaa tttcccacca ctattgttga
    10441 attccgaatt tcatcctgta tgacaaaaga atggctgtag ggtggggaaa aaacacaaag
    10501 taaacaataa ttgacgaata tagcgccatg ctgttcgcaa cctaaccaaa cagtcacttt
    10561 cgagcaattt tccttgaaaa agaggttgac gctgcaaggc tctatacgca taatgcgccc
    10621 cgcaacgccg ataaggtatc gcgaaaaaaa agatggctac gtagctcagt tggttagagc
    10681 acatcactca taatgatggg gtcacaggtt cgaatcccgt cgtagccacc atcttttttg
    10741 cgggagtggc gaaattggta gacgcaccag atttaggttc tggcgccgca aggtgtgcga
    10801 gttcaagtct cgcctcccgc accattcacc agaaagcgtt gtacggatgg ggtatcgcca
    10861 agcggtaagg caccggtttt tgataccggc attccctggt tcgaatccag gtaccccagc
    10921 catattcttc gaataagcgg ttcaccgccc ggttattggg gtatcgccaa gcggtaaggc
    10981 accggttttt gataccggca ttccctggtt cgaatccagg taccccagcc atcgaagaaa
    11041 caatctggct acgtagctca gttggttaga gcacatcact cataatgatg gggtcacagg
    11101 ttcgaatccc gtcgtagcca ccaaattctg aatgtgtcga atatgttcgg caaattcaaa
    11161 aaccaatttg ttggggtatc gccaagcggt aaggcaccgg attctgattc cggcattccg
    11221 aggttcgaat cctcgtaccc cagccaattt attcaagacg cttaccttgt aagtgcaccc
    11281 agttggggta tcgccaagcg gtaaggcacc ggattctgat tccggcattc cgaggttcga
    11341 atcctcgtac cccagccaca ttaaaaaagc tcgcctcggc gagctttttg cttttctgcg
    11401 tatattcaat gccgaatgcg atgttgactc gtcttatcct tcaaagtcgg atgcgacgct
    11461 gccgcgtctt atccgaccta cggttggcat gcatccagca atgttgtgag gctacaaccc
    11521 taacgcatat ttcagcgcct tacgtttcaa cacgccagca cgctccgccg ccatcaaacc
    11581 aagattacgc acaaaacgca gcggtggcag attattgctg aatccggcat aaaacagatc
    11641 cataccactt tgcatcataa agttatccgc catacgccgc atctggtaac gcttgagcac
    11701 aggataactg gcccacgctt cgccgtagct gcgggcgttc accagaacgt caatcagggc
    11761 atcgacatca cgataaccaa gattcacccc ctgccccgcc agcggatgga tggtatgcgc
    11821 ggcatcgccc accagagcaa gccctggttg cacatactgc aacgcatggc gacgtatcag
    11881 cggaaacgca ccagcggcaa gcggtgtcac gtaacccaga cgcgacggga aatgcttcgc
    11941 gatttccgcc tgtagctgcg ccatattcat attctgcaac tggcgaatgc gcgccggaga
    12001 gtcataccac accagcgatg cccagttatc aaacaacggc agaaacgcac gcggtccgtc
    12061 cggggtaaat tgctgccagg tgctgtcgcc gggatcgttc tcacactgta cgctaatcaa
    12121 catacacgac tgcgcatact gccaggcatg aacgccaatt cccgccatct gtcgcacctg
    12181 cgaatttgcg ccgtcggcac caatcaccag cttcgcgagg atcgtttcac cgcccttcag
    12241 ttccagctcc tgaagatcat tatggcgatg cagtgcaatc agcgaggttg gcacacgtaa
    12301 cgttactttc ggatgcgctt ccagcgcctg ccacaacgcc tgttgcagga cagtgttttc
    12361 caccatgtag ccaagcaacg gtagcttaag ttcagcggcg tcaaacacca catgcgccgt
    12421 ttcccactcc cacgtttcca gtctgcggta aggatggcaa cgcatagcct gtactgcatc
    12481 ccagacccct aaccctttaa gcaatgatac cgaagccgca ctaatcgccg agatccgcac
    12541 gtccggctgg ctgtcagcga caaacggtgc aggttgggca tgttcgataa ccattaccga
    12601 aaatccgtgc tgagccagtc ccagcgccag tgcgccgccg accattcctc cgccgacaat
    12661 ggcaatttcc gttggttgat ttgtcatggt caatcatcct gttagcaata tgctttaagt
    12721 ttacaggatt tttaccccca agggctgata acgctcatac tggtcacaac ggcagcaaag
    12781 cattacacta tgcgccctgc attcctggct actatttcgc aagagcaagt cgatgaccaa
    12841 aaaactccat attaaaacct ggggctgtca gatgaacgag tacgattcat cgaagatggc
    12901 cgatctgctg gatgccaccc acggctatca actgaccgac gtggcggaag aagcggatgt
    12961 gctgctgctg aacacctgct caatccgcga gaaggctcag gaaaaagtct tccatcagtt
    13021 gggtcgctgg aaactgttaa aagagaagaa tccagacctg attatcggcg tcggcggctg
    13081 cgtggcatcg caagaaggcg agcacattcg ccagcgcgcc cactatgtcg atattatttt
    13141 tgggccgcaa acgctgcacc gtctgccgga gatgatcaac tccgtgcgcg gcgaccgcag
    13201 cccggttgta gatatcagct tcccggaaat cgagaagttt gaccgtctgc cggaaccgcg
    13261 cgccgaaggg ccgaccgcgt ttgtctccat catggaaggc tgcaataaat attgcaccta
    13321 ctgcgtagtg ccttacaccc gtggtgaaga ggtaagccgt ccgtccgacg atattctgtt
    13381 tgagattgcc cagcttgctg ctcagggcgt gcgtgaagtt aacctgctcg gccagaacgt
    13441 gaacgcctgg cgcggtgaga actacgacgg caccaccgga tcgtttgccg atctgctgcg
    13501 tctggttgcg gcgatcgacg ggatcgatcg tattcgcttt accaccagcc atccgatcga
    13561 attcaccgac gatatcatcg aagtgtaccg cgacacgccg gagctggtga gcttcctgca
    13621 tctgccggta cagagcggtt ccgatcgcat tctgaacctg atgggacgta cccatacagc
    13681 gctggagtac aaagcgatca tccgtaagct gcgtgcggcg cgtccggata ttcagatcag
    13741 ttctgacttc atcgttggct tccctggcga aaccaccgaa gacttcgaga aaacgatgaa
    13801 gctgattgcc gacgtcaatt tcgacatgag ctacagcttt atcttctccg ctcgtccagg
    13861 tacaccagcc gccgatatgg ttgatgatgt tccggaagaa gagaagaagc agcgcctgta
    13921 tattctacaa gaacgtatca atcagcaagc aatggcatgg agccgccgga tgctcggtac
    13981 cacccagcgt attctggtag aaggtacatc gcgtaagagc atcatggagc tttccggccg
    14041 tactgaaaat aaccgcgtcg tcaacttcga aggtacgccg gatatgatcg gtaaattcgt
    14101 cgatgtagaa attaccgacg tctacccgaa ctctctgcgc ggtaaagtgg tgcgaactga
    14161 agatgaaatg ggtctgcgcg tggcagaaac accggaatcg gtgattgccc gtacccgcaa
    14221 agaaaacgac cttggcgtgg gttattatca gccgtaattc tcaggcccgc cgttccggtg
    14281 ggccttgttt tttctctctt tatccccatc tttatcgcaa tgcttgcgcc tttttcctgt
    14341 ggcgtgaata atttccttat aggccggtga atgttcagct tccggcggca tacagataag
    14401 aggaacggtt tgaacataga cactcgcgaa atcaccctgg agccagcaga caatgcgcgt
    14461 ctgttgagcc tgtgcggccc gtttgatgac aacatcaaac aactcgaacg ccgtctcggc
    14521 atcgaaatca atcgccgcga taaccacttt aaactgaccg gccgtccgat ttgcgtcacc
    14581 gctgcggcag acattctgcg tagcctgtat gtcgatactg ccccgatgcg cggtcagatt
    14641 caggatatcg aaccggaaca gatccacctt gcgattaaag aagcgcgggt actggagcaa
    14701 agcgcggaga gcgtgccgga gtacggcaaa gcggtcaata tcaaaaccaa acgcggcgta
    14761 attaagccgc gcacgccaaa ccaggcgcag tacatcgcca atattctcga ccatgacatt
    14821 accttcggcg ttggcccggc gggtacgggt aaaacctacc tggcagtggc tgcggcagtt
    14881 gatgccctgg agcgtcagga aattcgccgt attctgctga ctcgtccggc agtagaagcc
    14941 ggtgagaaac tgggcttcct gcctggcgat ttaagccaga aagtagaccc gtatctgcgc
    15001 ccgctgtacg acgcgctgtt tgaaatgctg ggctttgaga aagtcgagaa actgattgag
    15061 cgcaacgtta ttgaagtcgc accgctggcc tatatgcgtg gtcgtacgct gaacgacgcg
    15121 tttatcattc ttgatgagag ccagaacacc accatcgaac agatgaagat gttcctgacc
    15181 cgtatcggtt ttaactcaaa agcggttatc accggcgacg tcacacagat cgacctgccg
    15241 cgtaatacca agtctggttt gcgtcatgcc atcgaagtac tggctgatgt cgaagagatc
    15301 agctttaact tcttccacag cgaagacgtg gttcgtcacc cggtggtggc gcgtatcgtt
    15361 aacgcctatg aagcctggga agaagccgaa caaaaacgta aagcggcgct ggcagcagaa
    15421 cgcaagcgcg aagaacagga acaaaaatga gtcaggtgat cctcgattta caactggcat
    15481 gtgaagataa ttccgggtta ccggaagaga gccagtttca gacatggctg aatgcggtga
    15541 tcccgcagtt tcaggaagaa tcggaagtga ctattcgcgt ggtcgatacc gccgaaagcc
    15601 acagtctgaa tctgacctat cgcggtaagg ataagccgac caacgtgctc tccttcccgt
    15661 ttgaagtacc gcctggcatg gaaatgtcgc tactgggcga tctggttatc tgccgtcagg
    15721 tggttgagaa ggaagctcag gagcaaggca aaccactgga ggcgcactgg gcgcatatgg
    15781 tggtgcacgg cagtctgcat ttgttaggtt acgatcacat cgaagatgac gaagcagaag
    15841 aaatggaagc catcgaaaca gagattatgc ttgctctggg ctatgaggat ccgtacattg
    15901 ccgagaaaga ataatttgcc aggcatttga ctggcagacg tccccgccgt tggtgattaa
    15961 cgacggcgtt ttgattaact aacatgagaa cccatacgac gccatgagcg acgacaattc
    16021 acacagtagt gacacgataa gcaacaagaa gggatttttc tccctgttac tcagccaact
    16081 tttccacggt gaaccgaaaa accgtgacga actgctggcg ctgatccgtg attccgggca
    16141 gaacgacctt atcgacgaag atacgcgcga tatgctcgaa ggggtgatgg acatcgcaga
    16201 ccaacgcgtt cgcgacatca tgatcccccg ctcccagatg attaccctga aacgcaacca
    16261 gacgctggac gaatgccttg atgtcatcat cgagtccgcc cactcacgtt tcccggtgat
    16321 tagcgaagac aaagatcaca ttgaagggat tctgatggcg aaagacttgc tgccgtttat
    16381 gcgcagcgat gctgaagcct tcagcatgga caaagtgtta cgtcaggcgg ttgtcgttcc
    16441 tgaaagtaag cgcgtagacc ggatgctgaa agagtttcgc tctcagcgtt accacatggc
    16501 gatcgtcatt gacgaattcg gtggggtttc cggtctggtg accattgaag acatcctgga
    16561 actgattgtt ggtgagattg aagacgagta tgacgaagaa gatgatatcg acttccgtca
    16621 gctgagtcgt catacctgga ccgtgcgcgc actggcttct attgaagact tcaacgaagc
    16681 gttcggcacc cactttagcg atgaagaggt tgacactatc ggtggtctgg tgatgcaggc
    16741 atttgggcat cttccggcgc gtggcgaaac catcgacatc gacggttacc agttcaaagt
    16801 ggcgatggcc gacagtcggc gtattattca ggttcatgtc aaaatcccgg atgactcacc
    16861 ccagccgaag ctggatgaat aaaaccgaaa ctggatagat aactacatgg cttttgcctc
    16921 attaattgaa cgccagcgca ttcgcctgct gctggcgtta ttattcggtg cctgcggaac
    16981 gctggccttc tctccttacg acgtctggcc tgcggcgatt atttcgctga tggggcttca
    17041 ggcgttgacc tttaaccgcc gtccactcca gtctgccgct attggctttt gctggggatt
    17101 tggcctcttt ggcagcggta ttaactgggt ctatgtcagc atcgcgacct ttggcggaat
    17161 gcctggcccg gttaacatct tcctggtggt actgctggcg gcgtatttgt cgctgtatac
    17221 cggactgttt gctggcgtgc tgtcgcgtct gtggccgaaa accacctggc tgcgcgtggc
    17281 gattgccgcc cctgctctct ggcaagtgac cgagtttctg cgcggttggg tactaacagg
    17341 cttcccgtgg ttacagttcg gctatagcca gattgatggc ccgttaaaag ggctggcacc
    17401 gataatgggc gtggaagcca ttaacttcct gctgatgatg gttagcggcc tgctggcact
    17461 ggcgttggtc aaacgcaact ggcgtccgct ggtggtggcc gtcgtgctgt ttgcccttcc
    17521 cttcccgctg cgttacatcc agtggtttac cccgcaaccg gaaaaaacta ttcaggtttc
    17581 gatggttcag ggcgatattc cgcaatcgct gaaatgggac ggagaccagc tacttaatac
    17641 gctgaagata tactacaacg caacggcacc gctgatgggc aaatcatcgt tgattatctg
    17701 gccggagtcg gcgataaccg atctggaaat taatcagcaa ccgttcctca aagctctgga
    17761 cggtgagttg cgcgataaag ggagctcgct ggtcaccggg attgtcgacg cgcgtctcaa
    17821 taaacagaac cgctacgata cctacaacac catcatcaca ctgggtaaag gcgcaccgta
    17881 cagctatgaa tcagccgatc gctataacaa aaaccatctg gtgccgtttg gcgagtttgt
    17941 cccactggag tcgatactgc gtccgttagc accgttcttt gatttgccga tgtcttcatt
    18001 cagccgcgga ccatatattc agccaccgct gtcgttgaat ggcattcaac ttactgcggc
    18061 catttgctac gaaatcattc tcggcgagca agtgcgcgat aacttccgcc cggataccga
    18121 ctatctgctg actatctcca acgatgcgtg gtttggtaag tctattggtc catggcagca
    18181 cttccagatg gcgcgaatgc gtgcgctgga gctggcgcgc ccactgttgc gcagcaccaa
    18241 caacggcatt acggcggtga ttggcccgca gggtgagatt caggcgatga tcccgcagtt
    18301 cacccgcgag gtgttaacca ctaacgtgac gccgactacc ggactcacac cgtacgcacg
    18361 taccggcaac tggccgctgt gggtgctgac ggcactgttt ggttttgcag ctgtgctgat
    18421 gagtctgcga gcacgtaaac attaatgtgc ctgatgtatc cggcatgaac aaaacatact
    18481 ttctcattga gttgtggggc atgattatca tgcccttttt tcttacggtt cagggataca
    18541 agccacatcg ccatattggg gatgccgaat aattgcctgc ggatcaagcg agcgcgtcct
    18601 gtccatttgg attaacctgt tcttcgccat aacaaatatc tttctaccat ccgctgaaaa
    18661 acgaaggtga ttaacgtagc tggcaggata aacgccaata atctgcccgg cattaatatt
    18721 gagcaggatg acataacctt gcaggccata tgacaccaac gctaaatatt gaccgtccgg
    18781 gctaataccc tcaacggaat aaacgctcct gctacaccat tgcgtcataa tgctggcttg
    18841 ttgatcccct acgcttatac gtgaaatccc ctggctatcg gcaaaaataa tctcgccatt
    18901 ttttggtcgg aatcccatcg cagaaacgat accacgcgca tcacctgtgg gaaagtcttc
    18961 tttctgatgc gtgatggtat tgatgcgtac aatgtcgcct ctgaccatac cgggtatcaa
    19021 caatgttttt tcatccgggg aaagtgccat aacccaggcg cctggtttac cgctcgtccc
    19081 cgtcccgtcg tatttctcca gcgggatatc cgtcacatgc tgataagtca ccagatcaat
    19141 ggctgacaca ctattactgg tgcgattcat cacccaggct tttgtgccat cacgcgagag
    19201 gataaggctg tcaggatggg cgccggtggt aatcgactga ataatctccc ccgtcgccac
    19261 gttaatgacc gatactgcat tcgcttcaaa tgaagtcgca taaatcaact tttcatccag
    19321 gcttaatgcc agcgatctga cgccctgatc tttacagcca ccacaatgtt tgactgactt
    19381 ttctttcgct atgggcgctg taaaggtatg caggattttc ttttgtccga ggtcgaaaac
    19441 aacaatgctg ttggtgtttt caattgcggc atacaacgta ttaccatccc gactgatcgc
    19501 catatcatgg atatcggcaa tctcagcgag taaatcacca ctggcctgct cacgacttac
    19561 cggagcggat aatgtttccc tgagggcgtt gcgctcctcc actaactgct gctttttttg
    19621 tcgtcgctct tgttcaatct cctgcggata aaacgcttca cgcaggcttt gcctgacctg
    19681 taaaactgat tcatcaatct gctgctgcac aaaccagata gtcccggtca gaagaagagt
    19741 gaccgcaaca ataatcccgc cttccgtaac gagccgattt tgcccaacta aacgcgcact
    19801 cagccaaccc aacgcgcccc ccgcaatcag gccgccaatg tggcaagcgt tatctattcc
    19861 tgcttgccgg gcaccataca acagtgtcag cgccaccata cccaacagag aaaatactcg
    19921 ccgctggttt ttatggtgtg tacccgcacc tgaagaaagc tgtgttgcta tcgcagcgcc
    19981 cgctattccc ataattgccc cggatgcccc aacgctgatg actatctgct cactttcgcg
    20041 taacgcataa caggcgctga tcagcccacc aaagatgccg gagaataacc agataatcag
    20101 gagtcgccat ttccccagaa tacgttccac cgctcccccc accaccagga gtgcaaacat
    20161 attcatcagc aaatgagaaa cactgctgtg cagaaagaca ctgctcaata agcgccagga
    20221 ttcacccgac aacgtgagag aagcaacgtt agcaccgaga tggataagtg cggattgatc
    20281 gacttcttca taactcatcc attgtgcgag gtaacaaaat atcccaacac agagcgcaaa
    20341 aaggataagg gttattgcgg gtaattgaac atttaacggc ttaaccgaag atgcagacat
    20401 aaggtgattc ttccttgaaa agacaggtaa aaagcatttt tcccgtgtgc agttatcatc
    20461 ccggcaaacg tcccatgccg aaaagataac cctgtccgaa atatgactat ttcagcctaa
    20521 cattacaatc gtaattaatt cacctataag tattttttgg catgtaagtt gctttattta
    20581 ctgtgtaagc aatgaaatca gccgcgacgc aaccgggttg caccaccaca aatcagcggt
    20641 agcaccaaaa aggtgcgatt taagaatttt gcttcggaat ggtgcggcgc atctcgcaaa
    20701 aacaaacaat aaaacagcaa tatctttaca tttagccaaa ctaaatgtta cctaacaagc
    20761 acaacactgc acaataaagt tgcagatgat aacaacacaa acactcacaa cgggtatcca
    20821 tgcgttctta acgcagaaga taaaggagtt ggatatgcaa ttacgtaaac ctgccacagc
    20881 aatcctggcc ctggcgcttt ccgcaggact ggcacaggca gatgacgccg ccccggcagc
    20941 aggcagtacg ctggacaaaa tcgccaaaaa cggcgtgatt gtcgtcggtc accgtgaatc
    21001 ttcagtacct ttctcttatt acgacaatca gcaaaaagtg gtgggctact cgcaggatta
    21061 ctccaacgcc attgttgaag cagtgaaaaa gaaactcaac aaaccagact tacaggtgaa
    21121 attgattccg atcacctcac aaaaccgtat tccactgctg caaaacggca ctttcgattt
    21181 tgaatgcggt tctaccacca acaatgccga acgccaaaaa caggcggctt tctctgacac
    21241 tattttcgtg gtcggtacgc gcctgctgac caaaaagggc ggcgatatca aagattttgc
    21301 cgacctgaaa ggcaaagccg tagtcgtcac ttccggcacc acctctgaag ttttgctcaa
    21361 caaactgaat gaagagcaaa aaatgaatat gcgcatcatc agcgccaaag atcacggtga
    21421 ctctttccgt accctggaaa gcggtcgtgc cgttgccttt atgatggatg atgctctgct
    21481 ggccggtgaa cgtgcgaaag cgaagaaacc agacaactgg gaaatcgtcg gcaagccgca
    21541 gtctcaggaa gcctatggtt gcatgttgcg taaagacgat ccgcagttca aaaagctgat
    21601 ggatgatacc atcgctcagg tgcagacctc cggtgaagcg gaaaaatggt ttgataagtg
    21661 gttcaaaaat ccaattccgc cgaaaaacct gaacatgaat ttcgaactgt cagacgaaat
    21721 gaaagcactg ttcaaagaac cgaatgacaa ggcactgaac taattacaag aaccaggggc
    21781 ggaaattcca gccctctcga ttgttacgta gcacggacag actatacgcc tggtggtcgt
    21841 tccccatcgg gcctgaaaac cgcaatacgc tgggtaacaa tcttcgaggg tagcagttaa
    21901 cgctgctacc ctcttttttc tggagtagat ttatgtctat agactggaac tggggtattt
    21961 ttttacaaca agccccgttc ggcaacacca cctatcttgg ttggatctgg agtggttttc
    22021 aggtgacgat cgctttgtcg atctgcgcct ggattatcgc tttcctcgtc ggttcatttt
    22081 tcggcatttt acgtaccgtt ccaaaccgtt ttctctccgg tctgggtacg ttgtatgtcg
    22141 aactgttccg caacgtgccg ttgattgtgc aattctttac ctggtacctg gtgatcccgg
    22201 agctgctgcc agagaaaatc ggcatgtggt ttaaggcaga gctggatccc aatattcagt
    22261 ttttcctttc atccatgctc tgcctggggc tgtttaccgc cgcccgtgtt tgcgaacagg
    22321 tacgcgccgc tattcagtcg ctgccgcgtg ggcaaaaaaa tgccgcgctg gcgatgggtc
    22381 tgacgctacc gcaggcttac cgctacgtac tgctgcctaa tgcttatcgc gttatcgtcc
    22441 cgccgatgac ctcagagatg atgaacctgg tgaaaaactc cgccatcgcc tcgactatcg
    22501 gtctggtgga tatggcggcg caggcgggta aattgctcga ttactcggcc cacgcctggg
    22561 agtcgtttac cgccatcacg ctggcgtatg tattgattaa cgcttttatc atgctggtga
    22621 tgacgctggt tgaacgtaaa gttcgtctgc ccggcaacat ggggggcaaa taatgtacga
    22681 gtttgactgg agttccattg ttccttccct gccatatctg ctcgacgggc tggtgatcac
    22741 gctgaaaatc accgtcacgg cagtggtgat tggtattttg tggggcacca tgctggcggt
    22801 gatgcgcttg tccagctttg cgcctgttgc gtggtttgcc aaagcctatg ttaacgtatt
    22861 ccgctcgatt cctttagtca tggttttgct gtggttttac ctgattgtgc cgggatttct
    22921 gcaaaacgtg ctgggattat cgccaaaaaa tgatatccgt ttaatttcgg caatggtggc
    22981 gttttcgatg tttgaagcgg cctactattc agagattatc cgtgccggta ttcaaagtat
    23041 ttcccgtggt cagtcgagcg ccgcgctggc gttggggatg actcactggc agtcgatgaa
    23101 actgattatt ctgccgcagg ctttccgcgc gatggtgccg ctgctgctca ctcagggcat
    23161 cgtactgttc caggatacct cgctggtgta tgtgttatct ctggccgatt tcttccgtac
    23221 cgcttcaacc attggtgagc gtgatggtac gcaggttgag atgatcctgt ttgccggatt
    23281 tgtttatttc gttattagcc ttagcgcgtc gttgttggtc agctacttga aaagaaggac
    23341 agcataatga ttaccctgaa aaatgtttca aaatggtatg gtcactttca ggtgctgacc
    23401 gactgctcaa ccgaagtgaa aaaaggcgaa gtggtggtgg tttgcggccc gtctggttcc
    23461 ggcaaatcaa cgctgattaa aaccgtcaac ggcctcgaac cggtgcagca aggtgaaatc
    23521 accgtcgatg gcatcgtggt taacgacaag aaaaccgatc tggcaaagct gcgttcccgc
    23581 gtcgggatgg tattccagca tttcgagctg ttccctcatc tgtcgattat cgaaaacctg
    23641 accctggcgc aggtgaaagt gcttaaacgc gataaagcgc cggctcgtga aaaagcattg
    23701 aaattgctgg agcgcgtcgg gctttccgca catgccaata agtttccggc gcagctttcc
    23761 ggcggtcagc agcagcgtgt ggcgatcgct cgcgcattgt gtatggatcc tattgccatg
    23821 ctgttcgacg aaccgacatc ggcgctggat ccggagatga tcaacgaagt gctggacgtg
    23881 atggtggaac tggcgaacga aggaatgacc atgatggtgg tgacccacga aatgggcttt
    23941 gcccgtaaag tggcgaatcg ggtgatcttt atggacgagg gtaaaattgt cgaagactcg
    24001 ccgaaagacg ctttcttcga tgatccgaaa tcggaccgcg caaaagactt cctcgcgaaa
    24061 atcctgcatt aatcactctg gcgcgcatac ccaagtgcgc gccatctcac gctttatcgt
    24121 ttcgctcctc gtatctgtta catcctagcg gtacccttgt cgcaaagaag cacacaacaa
    24181 ggagcaacaa tggcactgcc aattctgtta gattgcgacc caggtcatga cgacgctatc
    24241 gcaatagttc ttgccctcgc ctcaccagag cttgatgtca aagcaattac gtcttccgcc
    24301 ggaaaccaga caccagaaaa aaccttacgc aatgttctgc gtatgctgac cttgcttaat
    24361 cgcaccgata ttccggtagc aggcggcgcg gtaaaaccgt taatgcgtaa tttgattatt
    24421 gcggacaatg tgcacggcga aagcggtctc gacggcccgg cattaccgga accgacgttc
    24481 gcaccgcaaa actgtacggc ggtggagcta atggcgaaaa cgctgtgtga aagtgaggaa
    24541 cctatcacca ttgtttcaac cggaccgcaa actaacgttg ccttgctgct caatagccac
    24601 ccggaactgc atagcaaaat tgcccgtatc gtgattatgg gcggcgcaat ggggcttggt
    24661 aactggacgc ctgcggctga atttaatatt tacgttgacc cggaagcggc agaaattgtc
    24721 ttccagtcag gaattccggt ggtgatggcc ggtctggatg ttactcacaa agcacaaatc
    24781 cacgttgaag acactgagcg tttccgcgcg attggcaacc ctgtttcaac cattgttgcc
    24841 gaactgctgg atttcttcct cgaatatcat aaagacgaaa aatggggctt tgtcggcgca
    24901 ccactgcatg acccatgcac catcgcctgg ctgttgaaac cggagttgtt taccactgtt
    24961 gagcgctggg ttggcgtgga aacacaaggg aaatataccc agggtatgac ggttgttgat
    25021 tattattatc tgactggcaa taaaccgaat gccaccgtaa tggtcgatgt tgatcgtcag
    25081 ggctttgttg atttactggc tgatcgtctg aaattttacg cttaatcact cgccggatga
    25141 catcacgcca tccggcacca ctcatttcat tacggctcga acggacgtct ctggaactga
    25201 tcatgaccac atttcgggca tagcgtcagc acttccggtg tatagatcgg gagatggaag
    25261 tgacatttct cgcagaccag atttcccagc cctaccactt ctccgctgtg ataaaccccg
    25321 tgatgattga ggtcctggaa aacttcgcgc cattcaagct gcgttttatc ggtgatgtct
    25381 gccagctcct gccacaagct ttctttaatc acccgcataa agacgctgtc agattcttct
    25441 ttcaggctct cttcatagct catggcgaac tcttccaggt cacgtctgac agctcgcgtc
    25501 agctcatcga cctcggttcg cgttaactcc cctgttttta ttacgcgctc gcgcgcctgt
    25561 tccaccagtg cgtcgatatc acgttcgcca ttgcgcaggc gttcgctcag tgacgcgacc
    25621 agttcacggt aatattgagc aaccttgttc atcgtttcat ctcccgggtc gttaactgac
    25681 tataataata gacgttcttt gttccttgct ttgctaatac gcccacaaac tctgtaaatt
    25741 aatgtatgaa taatccgggc atcatttcaa cgtcgagtgc gcgaaaggct gttttgacgc
    25801 ggacgtttgg gctatgctat gcggatctga aaaaccacct caacgctaca tttgtagccg
    25861 tattgaaaac aggaccactg gctgccatgc aagagcaata ccgcccggaa gagatagaat
    25921 ccaaagtaca gcttcattgg gatgagaagc gcacatttga agtaaccgaa gacgagagca
    25981 aagagaagta ttactgcctg tctatgcttc cctatccttc tggtcgacta cacatgggcc
    26041 acgtacgtaa ctacaccatc ggtgacgtga tcgcccgcta ccagcgtatg ctgggcaaaa
    26101 acgtcctgca gccgatcggc tgggacgcgt ttggtctgcc tgcggaaggc gcggcggtga
    26161 aaaacaacac cgcaccggca ccgtggacgt acgacaacat cgcgtacatg aaaaaccagc
    26221 tcaaaatgct gggctttggt tatgactgga gccgcgagct ggcaacctgt acgccggaat
    26281 actaccgttg ggaacagaaa ttcttcaccg agctgtataa aaaaggcctg gtatataaga
    26341 agacttctgc agtcaactgg tgcccgaacg accagaccgt actggcgaac gaacaagtta
    26401 tcgacggctg ctgctggcgc tgcgatacca aagttgagcg taaagagatc ccgcaatggt
    26461 ttatcaaaat caccgcttac gctgacgagc tgctcaacga tctggataaa ctggatcact
    26521 ggccagacac cgttaaaacc atgcagcgta actggatcgg tcgttccgaa ggcgtggaaa
    26581 tcaccttcaa cgttaacgac tatgacaaca cgctgaccgt ttacactacc cgcccggaca
    26641 cctttatggg ttgtacctac ctggcggtgg ctgcgggtca tccgctggca cagaaagcgg
    26701 cggaaaataa tcctgaactg gccgccttta ttgacgaatg ccgtaacacc aaagttgccg
    26761 aagctgaaat ggcgacgatg gagaaaaaag gcgtcgatac tggctttaaa gcggttcacc
    26821 cattaacggg cgaagaaatt cccgtttggg cagcaaactt cgtattgatg gaatacggca
    26881 cgggcgcagt tatggcggtt ccgggtcacg accagcgcga ctacgagttt gcctctaaat
    26941 acggcctgaa catcaaaccg gttatcctgg cagctgacgg ctctgagcca gatctttctc
    27001 agcaagccct gactgaaaaa ggcgtgctgt tcaactctgg cgagttcaac ggtcttgacc
    27061 atgaagcggc cttcaacgcc atcgccgata aactgactgc gatgggcgtt ggcgagcgta
    27121 aagtgaacta ccgcctgcgc gactggggtg tttctcgtca gcgttactgg ggcgcgccga
    27181 ttccgatggt gacgctggaa gacggtaccg taatgccgac cccggacgac cagctgccgg
    27241 tgatcctgcc ggaagatgtg gtgatggacg gcattaccag cccgattaaa gcagatccgg
    27301 agtgggcaaa aactaccgtt aacggtatgc cagcgctgcg tgaaaccgac actttcgaca
    27361 cctttatgga gtcctcctgg tactatgcgc gctacacttg cccggagtac aaagaaggta
    27421 tgctggattc caaagcggct aactactggc tgccggtgga tatctacatt ggtggtatcg
    27481 aacacgccat tatgcacctg ctctacttcc gcttcttcca caaactgatg cgtgatgcag
    27541 gcatggtgaa ctctgacgaa ccagcaaaac agttgctgtg tcagggtatg gtgctggcag
    27601 atgccttcta ctatgttggc gaaaatggcg aacgtaactg ggtttccccg gttgatgcta
    27661 tcgttgaacg tgacgagaaa ggccgtatcg tgaaagcgaa agatgcggca ggccatgaac
    27721 tggtttatac cggcatgagc aaaatgtcca agtcgaagaa caacggtatc gacccgcagg
    27781 tgatggttga acgttacggc gcggacaccg ttcgtctgtt tatgatgttt gcttctccgg
    27841 ctgatatgac tctcgaatgg caggaatccg gcgtggaagg ggctaaccgc ttcctgaaac
    27901 gtgtctggaa actggtttac gagcacacag cgaaaggtga tgttgcggca ctgaacgttg
    27961 atgcgctgac tgaagatcag aaagcgctgc gtcgcgatgt gcataaaacg atcgctaaag
    28021 tgaccgatga tatcggccgt cgtcagacct tcaacaccgc aattgcggcg attatggagc
    28081 tgatgaacaa actggcgaaa gcaccgaccg atggcgagca ggaccgcgcc ctgatgcagg
    28141 aagcactgct ggccgttgtc cgtatgctta acccgttcac cccgcacatc tgcttcacgc
    28201 tgtggcagga actgaaaggc gaaggcgata tcgacaacgc gccgtggccg gttgctgacg
    28261 aaaaagcgat ggtggaagac tccacgctgg tcgtggtgca ggttaacggt aaagtccgtg
    28321 caaaaatcac cgttccggtg gacgcaacgg aagaacaggt tcgcgaacgt gctggccagg
    28381 aacatctggt agcaaaatat cttgatggcg ttaccgtgcg taaagtgatt tacgtacccg
    28441 gtaaactcct caatctggtc gttggctaag cgcgggagga agcgtgcgat atctggcaac
    28501 attgttgtta tctctggcgg tgttaatcac cgccgggtgt ggctggcatc tgcgtgatac
    28561 cacgcaggtt ccttccacta tgaaggtcat gatcctggac tcaggcgatc cgaacgggcc
    28621 attaagccgt gcggtgcgta accagttacg tctaaatggt gtcgagttgc ttgataaaga
    28681 aaccacgcgt aaggacgttc catccctgcg tttgggtaaa gtgagcatcg cgaaagatac
    28741 cgcatcggta ttccgtaacg gtcaaacagc agagtatcag atgatcatga cggtcaatgc
    28801 gaccgtgttg atccccggcc gtgacatcta cccgattagc gccaaagtct tccgttcgtt
    28861 cttcgataac ccgcaaatgg cgttagcgaa agataacgaa caagacatga tcgtaaaaga
    28921 gatgtacgac cgtgctgccg aacagctgat tcgtaagctg ccaagcatcc gtgctgcgga
    28981 tattcgttcc gacgaagaac agacgtcgac cacaacggat actccggcaa cgcctgcacg
    29041 cgtctctacc acgctgggta actgatgatt cggttgtacc cggaacaact ccgcgcgcag
    29101 ctcaatgaag ggctgcgcgc ggcgtatctt ttacttggta acgatcctct gttattgcag
    29161 gaaagccagg acgctattcg tcaggtagct gcggtacaag gattcgaaga acaccacact
    29221 ttttccattg atcccaacac tgactggaat gcgatctttt cgttatgcca ggccatgagt
    29281 ctgtttgcca gtcgacaaac gctattgctg ttgttaccag aaaacggacc gaatgcggcg
    29341 atcaatgaac aacttctcac actcaccgga cttctgcatg acgacctgct gttgatcgtc
    29401 cgcggcaata aattaagcaa agcgcaagaa aatgccgcct ggtttactgc tcttgcgaat
    29461 cgcagcgtgc aggtgacctg tcagacaccg gagcaggctc agcttccccg ctgggttgct
    29521 gtgcgcgcaa aacagctcaa cttagagctg gatgacgcgg cgaatcaggt gctctgctac
    29581 tgttatgaag gtaacctgct ggcgctggct caggcactgg agcgtttatc gctactctgg
    29641 ccagacggca aactgacatt acctcgcgtt gaacaggcgg tgaatgatgc cgcgcatttc
    29701 accccttttc attgggttga tgctttgttg atgggaaaaa gtaagcgcgc gttgcatatt
    29761 cttcagcaac tgcgtctgga aggcagcgaa ccggttattt tgttgcgcac attacaacgt
    29821 gaactgttgt tactggttaa cctgaaacgc cagtctgccc atatgccact gcgtgcgttg
    29881 tttgataagc atcgggtatg gcagaaccgc cggggcatga tgggcgaggc gttaaatcgc
    29941 ttaagccatc cgcagttacg tcaggccgtg caactcctga cacgaacgga actcaccctc
    30001 aaacaagatt acggtcagtc agtgtgggca gagcttgaag ggttatctct tctgttgtgc
    30061 cataaacccc tggcggacgt atttatcgac ggttgatatg aaatctttac aggctctgtt
    30121 tggcggcacc tttgatccgg tgcactatgg tcatcttaaa cccgtggaaa cgctggcgaa
    30181 tttgattggt ctgacgcggg tcacaatcat ccctaataat gttcctccgc atcgtcccca
    30241 gccggaagca aacagcatgc agcgtaaaca catgcttgaa ctggcgattg ccgacaagcc
    30301 attatttact cttgatgaac gcgagctaaa gcgcaatgcc ccctcttaca ctgcgcaaac
    30361 actgaaagag tggcggcagg agcaaggacc ggacgtgccg ctggcgttta ttattggtca
    30421 ggattcattg ctgacctttc cgacctggta cgaatacgaa acgatactcg acaatgcaca
    30481 tttgatcgtc tgtcggcgtc ctggttaccc acttgaaatg gcacaaccac aatatcagca
    30541 atggctggag gatcatttga cacataaccc ggaagatctt caccttcagc ctgccggtaa
    30601 aatttatctg gctgaaacgc cgtggtttaa catctcggcg accatcatcc gcgaacgttt
    30661 gcaaaatggt gaatcgtgtg aggatttatt accggaaccg gtactgactt acattaacca
    30721 acaaggcttg tatcgctgat acctgtcgtt acggagggca tgatgcgact gtggttaatt
    30781 cgtcatggtg aaacgcaagc gaatgtcgat ggtctgtaca gcggtcatgc gcccacaccc
    30841 ctgaccgcgc gcggtattga gcaagcgcaa aatctgcata cgctgctaca tgatgtttcc
    30901 tttgacctgg ttttatgcag tgaactggaa cgggcacagc ataccgcgcg actggttctt
    30961 agtgaccgcc agcaccccgt tcatatcatc cctgaactca atgaaatgtt ttttggcgac
    31021 tgggagatgc gacatcatcg tgacctcatg caagaagatg ccgaaaacta tagcgcgtgg
    31081 tgcaatgact ggcagcatgc tatccccacg aacggtgaag gatttcaggc attttcgcaa
    31141 cgtgtggaac gatttatcgc aaggcttagt gaatatcagc actatcagaa tattttagtc
    31201 gtcagccatc agggcgtact gagtctgtta attgctcgtt taattggcat gcctgccgag
    31261 tctatgtggc atttccgcgt tgaccagggg tgttggagcg ccattgatat caaccaaaaa
    31321 ttcgcgacgc tacgcgtcct caatagccgt gccatcgggg tcgagaatgc atgacttttc
    31381 tgttttttta cgggtaagcc gcaacgacca ttgacagccc cgggcaggct gatattctcc
    31441 gcagccagac tttttccgcc agacacgact ttgtagaaat tgttttacaa aaatggcgat
    31501 gcaatctgcg gcgcggggtg ggatgatagc ccactttcga aagccgattc ggcgacaatt
    31561 gtcccgaaat cgcctctggt tcaggtatac tgacagacca tttttatcta tttgattcac
    31621 ccagggggaa aacttgcagg gtaaagcact ccaggatttt gttatcgaca aaattgatga
    31681 cctcaaaggt caggacatca tcgccttaga cgttcagggc aaatccagca tcaccgactg
    31741 catgatcatc tgtacgggta cgtccagccg ccatgttatg tccattgctg atcacgttgt
    31801 gcaggagtct cgcgcagcgg gcctgttacc gctcggcgta gaaggtgaaa acagcgccga
    31861 ctggattgtc gtggatttgg gcgatgtgat tgtccatgtc atgcaggaag agagccgtcg
    31921 cctgtatgaa ctggaaaaac tctggagtta atgcgtgaag ctgcaacttg tcgccgtggg
    31981 aacgaaaatg ccggactggg tacaaaccgg ttttaccgag tacctgcgtc gttttccgaa
    32041 agatatgccc ttcgagctga ttgaaattcc ggccggaaaa cgcggcaaga atgcggacat
    32101 caagcgcata ctcgacaaag agggtgagca gatgttggcg gccgcaggca aaaaccgcat
    32161 tgtcaccctc gatattccag gcaagccctg ggatacgccg cagttagccg ctgagctgga
    32221 acgctggaag ctggatggtc gcgacgtcag tctactgatt ggcgggcctg aagggttgtc
    32281 gcctgcctgt aaagcggcgg cggagcagag ctggtcgctg tcggcactta cccttcccca
    32341 tccgctggtt cgcgtgctgg tcgcagagag tctgtaccgg gcgtggagca tcaccaccaa
    32401 ccatccttat caccgtgagt gataagtgag ctttgagtag aaaacgcagc ggatgaaact
    32461 acagaactct tttcgcgact atacggcaga gtccgcgctg tttgtgcgcc gggcgctggt
    32521 cgcctttttg gggattttgc tgctgaccgg cgtgcttatc gccaacctgt ataatctgca
    32581 aattgttcgc tttaccgact accagacccg ctctaatgaa aaccgcatta agctggtgcc
    32641 tatcgcaccc agccgcggca ttatctacga ccgtaacggt atccctctgg ctctcaaccg
    32701 cactatctac cagatagaaa tgatgccgga gaaagtcgat aacgtgcagc aaacgctgga
    32761 cgctttgcgc agcgtggttg atctgaccga tgatgatatt gcggcattcc gaaaagagcg
    32821 cgcacgttca caccgtttca cctctattcc ggtgaaaact aacctgaccg aagtacaagt
    32881 agctcgcttt gccgtcaacc agtaccgttt tccgggtgtt gaagttaaag gctataaacg
    32941 tcgttactat ccttacggtt cggcgttgac ccacgtcatc ggctatgtgt cgaaaatcaa
    33001 cgataaagac gtcgaacgcc tgaataatga cggcaaactg gccaactatg cggcaacgca
    33061 tgatatcggt aagctgggca ttgaacgtta ctatgaagac gtgctgcacg gtcagaccgg
    33121 ttatgaagag gttgaagtta acaaccgtgg gcgtgttatc cgccagttaa aagaagtacc
    33181 accgcaagcc ggacacgata tttacctgac gctggatctc aaactccagc aatatattga
    33241 aacgctgctg gcgggtagcc gcgcagctgt ggtagtcacc gatccgcgta caggtggggt
    33301 gttggcgctg gtttccacgc ctagttatga cccaaacctg tttgttgacg gtatctccag
    33361 caaagattat tccgccttgc tgaacgaccc gaatacaccg ctggtgaacc gcgccacaca
    33421 gggggtttat cctcccgcgt ctacagttaa accctatgtt gcggtttcgg cattgagtgc
    33481 cggggtgatc acgcgcaata cgacgctgtt tgaccccggc tggtggcaac tgccagggtc
    33541 ggaaaaacgt tatcgtgact ggaaaaaatg gggccacggg cgtctgaatg tcacaagatc
    33601 gctggaagaa tctgcggata ccttcttcta tcaggtggcc tacgatatgg ggatcgatcg
    33661 cctctccgaa tggatgggta aattcggtta tggtcattac accggtatcg acctggcgga
    33721 agaacgttcc ggcaacatgc ctacccgcga atggaaacag aaacgcttta aaaaaccgtg
    33781 gtatcagggt gacaccattc cggttggtat cggtcagggt tactggacag cgaccccaat
    33841 ccagatgagt aaggcactga tgatcctgat taatgacggt atcgtgaagg ttccgcattt
    33901 gctgatgagc accgccgaag acggcaaaca ggtgccatgg gtacaaccgc atgaaccgcc
    33961 cgtcggcgat attcattccg gttactggga gctggcgaaa gacggtatgt acggtgttgc
    34021 taaccgccct aacggtacgg cgcataaata ttttgccagc gcaccgtaca aaattgcggc
    34081 gaaatccggt accgctcagg tcttcggtct gaaagcgaac gaaacctata atgcgcacaa
    34141 aattgccgag cgtttacgtg accacaaact gatgaccgcc tttgcgccat acaacaatcc
    34201 gcaagtggct gtcgccatga ttctggagaa cggtggcgcg ggtccggcgg ttggtacgct
    34261 gatgcgccag atcctcgacc acattatgct gggtgataac aacaccgatc tgcctgcgga
    34321 aaatccagcg gttgccgcag cggaggacca ttaatcatga cagataatcc gaataaaaaa
    34381 acattctggg ataaagtcca tctcgatccc acaatgctgc tgatcttact ggcattgctg
    34441 gtttacagcg ccctggttat ctggagcgcc agcggtcagg atattggcat gatggagcgt
    34501 aaaatcggcc agattgcgat gggtctggtc atcatggtgg tgatggcgca aattcctcca
    34561 cgcgtttatg aaggctgggc cccctatctc tatatcatct gtattatttt gctggtggcg
    34621 gtagatgctt tcggtgccat ctctaaaggc gctcaacgct ggctggacct cggtattgtt
    34681 cgttttcaac cgtcggaaat tgccaagata gccgtaccgt tgatggttgc acgctttatc
    34741 aaccgcgacg tttgcccacc ttcattgaag aatacagcta tcgcgctggt gctgatattt
    34801 atgcccacgc tgctggtggc tgcacagcct gacttgggaa catcaatcct cgttgcgctt
    34861 tccggcctgt ttgtactgtt cctctctggc cttagctggc gtctgattgg cgtcgcagta
    34921 gtgctggtag cggcgttcat tccgattctg tggttctttc tgatgcatga ttaccagcgc
    34981 cagcgcgtaa tgatgctcct ggacccggaa tcagacccac tcggcgcagg ctatcacatt
    35041 attcagtcta aaattgctat tggctccggc ggattacgcg gcaaaggctg gctgcacggc
    35101 actcagtcac agcttgaatt tctccccgaa cgccataccg actttatctt cgcggtactg
    35161 gcggaagagc tgggattagt gggcattttg attctgctcg ctctctacat tctgctgatc
    35221 atgcgcgggc tgtggatagc cgccagagcg caaaccacct ttggtcgcgt catggctggc
    35281 ggcttaatgc tgatattatt cgtttatgtc ttcgtaaata ttggtatggt aagcggtatt
    35341 ctgccggttg taggggttcc gctcccactg gtcagttatg gaggatcggc gctaattgtg
    35401 ctgatggctg ggttcgggat tgtaatgtca atccacaccc acaggaaaat gttgtcgaaa
    35461 agcgtgtaag aggtgcgcaa tgcgtaagca gtggctcggg atctgcatcg cggcaggaat
    35521 gctcgcggca tgtacaagcg atgatggtca gcaacagacg gtaagtgtac cgcagcctgc
    35581 ggtatgtaac ggccccatag ttgaaattag cggggcagac ccgcgtttcg aaccactgaa
    35641 cgcgacggca aatcaggatt accagcgcga cgggaaaagc tacaaaatcg tgcaggatcc
    35701 gtctcgattt agccaggcgg gactggcggc aatctatgat gccgaacccg gcagtaacct
    35761 gacggcctct ggcgaagctt tcgatccgac acagttgacg gcggcccatc caacgcttcc
    35821 aatccccagc tacgccagaa tcactaacct ggctaacggg cgaatgatcg tggtgcgcat
    35881 taatgatcgc ggcctttatg gcaacgaccg cgtgatttcg ctttctcgcg cggcagctga
    35941 ccgtcttaac acgtcaaaca acaccaaagt tcgtatcgat ccgattattg tcgcccagga
    36001 tggttcgctt tctggtcctg gtatggcttg taccacagtc gccaaacaga cttacgccct
    36061 gccagcacct cccgatttaa gcggtggcgc gggaacaagt tcagtgcctg tcccgcaggg
    36121 tgacattctt ccggtcagta attcgacgct aaaaagcgaa gatccgaccg gcgcgccggt
    36181 aaccagcagc ggtttcctcg gcgcaccaac gaccttagcg cctggtgtac tggaaggcag
    36241 cgaaccgacg cctgcgccac agcccgttgt tacagctccg tcgacaacgc ctgcaacctc
    36301 acctgcaatg gtgacaccgc aagccgcctc gcaaagcgcc agcggcaact ttatggtgca
    36361 agtcggggcc gttagcgatc aggctcgtgc gcaacagtac caacagcaac tgggacagaa
    36421 gttcggcgtc cccggtcgcg taactcaaaa tggcgcggtc tggcggatcc agcttggccc
    36481 attcgccagc aaagccgaag ccagtacctt gcagcaacgt ttgcaaaccg aagcccaatt
    36541 acagtcattt attaccaccg cgcagtagcg taaagcaggc atctgatgtg tcaatttctg
    36601 taaatgacgt taacaaagtc acgcggaaag tcagatgcct gccggtagtg catttgctat
    36661 agtagggcac tttttttaat tccatcacgg atgtcgtagt tcagaccatg aataccattt
    36721 tttccgctcg tatcatgaag cgcctggcgc tcaccacggc tctttgcaca gcctttatct
    36781 ctgctgcaca tgccgatgac ctgaatatca aaaccatgat cccgggtgta ccgcagatcg
    36841 acgcggagtc ctacatcctg attgactata actccggcaa agtactcgcc gaacagaacg
    36901 ctgatgtccg ccgcgatcct gccagcctga ccaaaatgat gaccagttac gttatcggcc
    36961 aggcaatgaa agccggtaaa tttaaagaaa ctgatttagt cactatcggc aacgacgcat
    37021 gggccaccgg taacccggtg tttaaaggtt cttcgctgat gttcctcaaa ccgggcatgc
    37081 aggttccggt ttctcagctg atccgcggta ttaacctgca atcgggtaac gatgcttgtg
    37141 tcgccatggc cgattttgcc gctggtagcc aggacgcttt tgttggcttg atgaacagct
    37201 acgttaacgc cctgggcctg aaaaataccc acttccagac ggtacacggt ctggatgctg
    37261 atggtcagta cagctccgcg cgagatatgg cgctgatcgg ccaggcgttg atccgtgacg
    37321 taccgaatga atactcgatc tataaagaaa aagaatttac gtttaacggt attcgccagc
    37381 tgaaccgtaa cggcctgtta tgggataaca gcctgaatgt cgacggcatc aaaaccggac
    37441 acactgacaa agcaggttac aaccttgttg cttctgcgac tgaaggccag atgcgcttga
    37501 tctctgcggt gatgggcgga cgtactttta aaggccgtga agccgaaagt aaaaaattgc
    37561 taacctgggg cttccgtttc ttcgaaaccg ttaatcctct gaaagtaggt aaagagttcg
    37621 cctctgaacc ggtttggttt ggtgattctg atcgcgcttc gttaggggtt gataaagacg
    37681 tgtacctgac cattccgcgt ggtcgcatga aagatctgaa agccagctat gtgctgaaca
    37741 gcagtgaatt gcatgcgccg ctgcaaaaga atcaggtcgt cggaactatc aacttccagc
    37801 ttgatggcaa aacgatcgaa caacgcccgc tggttgtgct gcaagaaatc ccggaaggta
    37861 acttcttcgg caaaatcatt gattacatta aattaatgtt ccatcactgg tttggttaaa
    37921 aattgaacac ttgaaagtgt aatttccgtc cccatatact aagcatcagt aaaaaactcc
    37981 cgccttctgg cgggagttgc tatttaatta cgttacgccg gagctgacat gaaaaccaaa
    38041 cttaacgaac tgcttgaatt ccctactcct tttacttaca aagttatggg gcaggcgtta
    38101 cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc atgcgccagg tgactacacc
    38161 ccaacggtaa aaccaagcag caaaggcaac taccactcgg tatctatcac tatcaacgcc
    38221 actcatatcg agcaggttga aacactgtat gaagaactgg gcaaaatcga tattgtccgc
    38281 atggttctgt aactcgcttc tccgttaccc ggctcctggt cgggtaactc ccctctcctc
    38341 gctgtgatat acttttcccc cacttttact cattctccac ggagatgccg ttttgtatca
    38401 ggataaaatt cttgtccgcc agctcggtct tcagccttac gagccaatct cccaggctat
    38461 gcatgaattc accgataccc gcgatgatag tacccttgat gaaatctggc tggtcgagca
    38521 ctatccggta ttcacccaag ggcaggcagg aaaggcggag cacattttaa tgccgggtga
    38581 tattccggtg atccagagcg atcgcggtgg gcaggtgact tatcacgggc cggggcaaca
    38641 ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc ggtgtgcgtg aactggtgac
    38701 cttgcttgag caaacagtgg tgaataccct ggctgaactg ggtatagaag cgcatcctcg
    38761 ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt tgctcactgg gtttacgtat
    38821 tcgacgcggt tgttcattcc acggtctggc attaaacgtc aatatggatc tttcaccatt
    38881 tttacgtatt aatccttgtg ggtatgcagg aatggaaatg gctaaaatat cacaatggaa
    38941 acccgaagcg acgactaata atattgcacc acgtttactg gaaaatattt tagcgctact
    39001 aaacaatcct gacttcgaat atattaccgc ttaattccat acatcaatgg cccaatttac
    39061 actgggtcat tactcataat acctgctaat gctttacatc tccttcgcgt ttgtatattt
    39121 tctttatcac ttgcagaaat gcgatgctac gccttgctcc gtagcccacc taattgatta
    39181 acccattgaa ataattgacc atggtgagca aaagcgtt
//
LOCUS       DAZKGF010000022        38356 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZKGF010000022 DAZKGF010000000
VERSION     DAZKGF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.022"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            59..1714
                     /gene="aslA"
                     /locus_tag="ACHOQV_001152"
     CDS             59..1714
                     /gene="aslA"
                     /locus_tag="ACHOQV_001152"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFP9629556.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMANAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1872..3107)
                     /gene="aslB"
                     /locus_tag="ACHOQV_001153"
     CDS             complement(1872..3107)
                     /gene="aslB"
                     /locus_tag="ACHOQV_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP9629557.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKVFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(3254..3330)
                     /locus_tag="ACHOQV_001154"
     tRNA            complement(3254..3330)
                     /locus_tag="ACHOQV_001154"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3294..3296),aa:Pro,seq:tgg)
     gene            complement(3373..3459)
                     /locus_tag="ACHOQV_001155"
     tRNA            complement(3373..3459)
                     /locus_tag="ACHOQV_001155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3423..3425),aa:Leu,seq:cag)
     gene            complement(3480..3555)
                     /locus_tag="ACHOQV_001156"
     tRNA            complement(3480..3555)
                     /locus_tag="ACHOQV_001156"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3520..3522),aa:His,seq:gtg)
     gene            complement(3614..3690)
                     /locus_tag="ACHOQV_001157"
     tRNA            complement(3614..3690)
                     /locus_tag="ACHOQV_001157"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3654..3656),aa:Arg,seq:ccg)
     gene            complement(3793..5178)
                     /gene="yifK"
                     /locus_tag="ACHOQV_001158"
     CDS             complement(3793..5178)
                     /gene="yifK"
                     /locus_tag="ACHOQV_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9629558.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPTAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEK"
     gene            complement(5369..6109)
                     /gene="wecG"
                     /locus_tag="ACHOQV_001159"
     CDS             complement(5369..6109)
                     /gene="wecG"
                     /locus_tag="ACHOQV_001159"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFP9629559.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6112..7464)
                     /gene="wzyE"
                     /locus_tag="ACHOQV_001160"
     CDS             complement(6112..7464)
                     /gene="wzyE"
                     /locus_tag="ACHOQV_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFP9629560.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7461..8540)
                     /gene="rffT"
                     /locus_tag="ACHOQV_001161"
                     /gene_synonym="wecF"
     CDS             complement(7461..8540)
                     /gene="rffT"
                     /locus_tag="ACHOQV_001161"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFP9629561.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCLALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDSSLNTMVNDRQREVKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8537..9787)
                     /gene="wzxE"
                     /locus_tag="ACHOQV_001162"
     CDS             complement(8537..9787)
                     /gene="wzxE"
                     /locus_tag="ACHOQV_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFP9629562.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQELVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9789..10919)
                     /gene="rffA"
                     /locus_tag="ACHOQV_001163"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9789..10919)
                     /gene="rffA"
                     /locus_tag="ACHOQV_001163"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFP9629563.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKYNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10924..11598)
                     /gene="rffC"
                     /locus_tag="ACHOQV_001164"
                     /gene_synonym="wecD"
     CDS             complement(10924..11598)
                     /gene="rffC"
                     /locus_tag="ACHOQV_001164"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFP9629564.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPEAPLLTPDALAPWSRV
                     QAKIAASNTDELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRSASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11576..12457)
                     /gene="rfbA"
                     /locus_tag="ACHOQV_001165"
     CDS             complement(11576..12457)
                     /gene="rfbA"
                     /locus_tag="ACHOQV_001165"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9629565.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTQGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12476..13543)
                     /gene="rffG"
                     /locus_tag="ACHOQV_001166"
     CDS             complement(12476..13543)
                     /gene="rffG"
                     /locus_tag="ACHOQV_001166"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9629566.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFVETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SVSKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13543..14805)
                     /gene="wecC"
                     /locus_tag="ACHOQV_001167"
     CDS             complement(13543..14805)
                     /gene="wecC"
                     /locus_tag="ACHOQV_001167"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFP9629567.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14802..15932)
                     /gene="wecB"
                     /locus_tag="ACHOQV_001168"
     CDS             complement(14802..15932)
                     /gene="wecB"
                     /locus_tag="ACHOQV_001168"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFP9629568.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(15988..17034)
                     /gene="wzzE"
                     /locus_tag="ACHOQV_001169"
     CDS             complement(15988..17034)
                     /gene="wzzE"
                     /locus_tag="ACHOQV_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFP9629569.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYFRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17046..18149)
                     /gene="wecA"
                     /locus_tag="ACHOQV_001170"
     CDS             complement(17046..18149)
                     /gene="wecA"
                     /locus_tag="ACHOQV_001170"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFP9629570.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18389..19648)
                     /gene="rho"
                     /locus_tag="ACHOQV_001171"
     CDS             complement(18389..19648)
                     /gene="rho"
                     /locus_tag="ACHOQV_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP9629571.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19733..19834)
                     /gene="rhoL"
                     /locus_tag="ACHOQV_001172"
     CDS             complement(19733..19834)
                     /gene="rhoL"
                     /locus_tag="ACHOQV_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFP9629572.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(19975..20304)
                     /gene="trxA"
                     /locus_tag="ACHOQV_001173"
     CDS             complement(19975..20304)
                     /gene="trxA"
                     /locus_tag="ACHOQV_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFP9629573.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20435..21700
                     /gene="rhlB"
                     /locus_tag="ACHOQV_001174"
     CDS             20435..21700
                     /gene="rhlB"
                     /locus_tag="ACHOQV_001174"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFP9629574.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAQATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21834..23318
                     /gene="gppA"
                     /locus_tag="ACHOQV_001175"
     CDS             21834..23318
                     /gene="gppA"
                     /locus_tag="ACHOQV_001175"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFP9629575.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLAQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFYDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23365..25386)
                     /gene="rep"
                     /locus_tag="ACHOQV_001176"
     CDS             complement(23365..25386)
                     /gene="rep"
                     /locus_tag="ACHOQV_001176"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFP9629576.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25473..25754
                     /gene="ppiC"
                     /locus_tag="ACHOQV_001177"
     CDS             25473..25754
                     /gene="ppiC"
                     /locus_tag="ACHOQV_001177"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFP9629577.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25800..27275)
                     /gene="ilvC"
                     /locus_tag="ACHOQV_001178"
     CDS             complement(25800..27275)
                     /gene="ilvC"
                     /locus_tag="ACHOQV_001178"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFP9629578.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETVTEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27425..28315
                     /gene="ilvY"
                     /locus_tag="ACHOQV_001179"
     CDS             27425..28315
                     /gene="ilvY"
                     /locus_tag="ACHOQV_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFP9629579.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLSTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLDNSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28312..29856)
                     /gene="ilvA"
                     /locus_tag="ACHOQV_001180"
     CDS             complement(28312..29856)
                     /gene="ilvA"
                     /locus_tag="ACHOQV_001180"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFP9629580.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29859..31709)
                     /gene="ilvD"
                     /locus_tag="ACHOQV_001181"
     CDS             complement(29859..31709)
                     /gene="ilvD"
                     /locus_tag="ACHOQV_001181"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFP9629581.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDDSALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31774..32703)
                     /gene="ilvE"
                     /locus_tag="ACHOQV_001182"
     CDS             complement(31774..32703)
                     /gene="ilvE"
                     /locus_tag="ACHOQV_001182"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFP9629582.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32723..32986)
                     /gene="ilvM"
                     /locus_tag="ACHOQV_001183"
     CDS             complement(32723..32986)
                     /gene="ilvM"
                     /locus_tag="ACHOQV_001183"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFP9629583.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32983..34629)
                     /gene="ilvG"
                     /locus_tag="ACHOQV_001184"
     CDS             complement(32983..34629)
                     /gene="ilvG"
                     /locus_tag="ACHOQV_001184"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFP9629584.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKSADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34632..34682)
                     /gene="ilvX"
                     /locus_tag="ACHOQV_001185"
     CDS             complement(34632..34682)
                     /gene="ilvX"
                     /locus_tag="ACHOQV_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFP9629585.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34769..34867)
                     /gene="ilvL"
                     /locus_tag="ACHOQV_001186"
     CDS             complement(34769..34867)
                     /gene="ilvL"
                     /locus_tag="ACHOQV_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFP9629586.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            35220..36740
                     /gene="yifB"
                     /locus_tag="ACHOQV_001187"
     CDS             35220..36740
                     /gene="yifB"
                     /locus_tag="ACHOQV_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFP9629587.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVQGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36766..37104)
                     /gene="maoP"
                     /locus_tag="ACHOQV_001188"
     CDS             complement(36766..37104)
                     /gene="maoP"
                     /locus_tag="ACHOQV_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFP9629588.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            37223..38062
                     /gene="hdfR"
                     /locus_tag="ACHOQV_001189"
     CDS             37223..38062
                     /gene="hdfR"
                     /locus_tag="ACHOQV_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFP9629589.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IATLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(38157..38232)
                     /locus_tag="ACHOQV_001190"
     tRNA            complement(38157..38232)
                     /locus_tag="ACHOQV_001190"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38197..38199),aa:Trp,seq:cca)
     gene            complement(38241..38317)
                     /locus_tag="ACHOQV_001191"
     tRNA            complement(38241..38317)
                     /locus_tag="ACHOQV_001191"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38281..38283),aa:Asp,seq:gtc)
BASE COUNT         9389 a        10481 c         9690 g         8796 t
ORIGIN      
        1 caattatcaa tgcttaatta cgtcatcatt ttgacgacat gaaaccggag cattttgcat
       61 ggaattttcg ttttcaccca aacttcttgt tgtggctgtc gccgccgctc ttccactcat
      121 ggccaacgca gcagataccc cgtcaaccgc caccgcacgc aaaggctttg ccggatacga
      181 tcacccaaac cagtatctgg ttaaaccggc gaccactatt gccgacaaca tgatgccggt
      241 gatgcagcat cctgcacaag acaaagaaac ccagcagaag ctggcagaac ttgagaaaaa
      301 aaccggtaag aaaccgaacg tggttgtttt cttgctggat gatgtgggct ggatggatgt
      361 tggctttaac ggcggcggcg tggcggtggg taaccctaca ccagatatcg acgccgttgc
      421 cagccagggg ctgattttaa cttcggcgta ttctcaaccg agttcttccc caacccgcgc
      481 cacgattctt accggacaat actccatcca ccacggcatt ctgatgccgc caatgtacgg
      541 gcaaccgggc ggtctgcaag ggttaaccac gctgccgcag ttgctgcacg atcagggcta
      601 cgtcactcag gccatcggga aatggcatat gggggaaaac aaagagtcgc agccgcagaa
      661 cgttggcttt gatgatttcc gcggctttaa ctcggtatcc gatatgtata ccgaatggcg
      721 tgatgttcac gtcaacccgg aagtcgccct gagcccggat cgttctgaat acatccaaaa
      781 actgccgttc agcaaagatg acgttcatgc cgtgcgcggc ggtgaacagg aagccattgc
      841 cgacattacg ccgaaatata tggaagacct ggatcaacgc tggatggaat atggcgttaa
      901 gttcctcgac aagatggcga agagcgataa gcctttcttc ctctactacg gcactcgcgg
      961 ctgtcacttc gataactacc caaatgcgaa atatgcgggt agctctccgg cgcgcacctc
     1021 ttacggcgac tgcatggtgg agatgaacga tatcttcgcc aatctgtata aagcactgga
     1081 gaaaaacggt cagcttgata acacgctgat tgtgttcacc tctgataacg gtccggaagc
     1141 cgaagttccg ccgcacggtc gcacaccgtt ccgcggtgcg aaaggttcta catgggaagg
     1201 cggcgttcgt gtgccaacct tcgtttactg gaaaggcatg atccaaccgc gtaaatctga
     1261 cggcattgtc gatctggcgg atctcttccc tacggcgctg gatctggcgg ggcatcctgg
     1321 ggcgaaagta gcgaatttag taccgaaaac cacctttatc gatggtgtgg accagacatc
     1381 tttcttcctg ggaacaaatg gtcagtctaa ccgtaaggcc gagcactact tcctcaacgg
     1441 taaactttct gctgtgcgta tggatgagtt caagtatcac gtcctgattc agcaacctta
     1501 cgcttatacc cagagcggat atcagggtgg attcaccggc acagtaatgc aaacggcggg
     1561 atcgtcggtg tttaacctgt acaccgatcc gcaggaaagc gactctatcg gcgtgcgcca
     1621 tattccgatg ggtgtaccgc tacagaccga aatgcacgcg tatatggaga tcctgaaaaa
     1681 atatccacca cgcgcgcaga ttaaatctga ctaagccggc gctatcgctg agatctgcct
     1741 ttgccggatg cgatgctgac gcatcttatc cagcctacag aacgctgcaa tttattgaat
     1801 ttgcaatgat tgtaggccgg ataaggcgtt cacgccgcat ccggcaatca accgcaggcg
     1861 gccgccgatt tctacttatt caccaccatc aaatgcgcct gcataatgtc gctggccgga
     1921 cgaccgtgcg ccagcaaatc agccattgct ttaagatatg gcggtagatg gcggaaataa
     1981 cgctgatacc cggcacacaa ataattcagc ccgggtttgc cgctggcatc gagcataaag
     2041 cggtgtttcg ggcagcctcc ccagcacgct tttaacacgt tgcaactgcg acactgtgcc
     2101 ggtaactgct tgaatttatc ttcaccaaat gcctgctgtt gcggggaatc aatcatttct
     2161 gcaatcgtct gctgaagcat attccctagt cgatactgcg gataaacata gtgatcgcag
     2221 gcgtaaacat cgccgttgtg ttcaacaatc accgagcgcc cacaggttgg ctgatgatgg
     2281 caaaccgcgc ccggtgcgcc gacaaaattg gcgaacgccc attcgatatt cattacgaaa
     2341 accttgccga cgtcgcgctt aatccagtgg tcgaaaatcg ccaccagaaa ctcaccgaac
     2401 tcttgcgggc acaccgacca ttccgtcagt tcgccctgaa tatcgccggg agcatgtagc
     2461 ttaagtccag catgagcagc cgtttcatca gccaggcgct cgaccaccgg aataaactgg
     2521 atgaactcaa cacctgcatc acacagaaaa tcatagactt gcaaaggttg taacgcactg
     2581 gtgcgattta cgcagaccag cacgttatag tcgacatgat gtttttgcag gagcgtcagg
     2641 gcacgcatca ccagcttatg cgttggtctg ccgcctttgg tcacgcgata ttgattgtgg
     2701 atctcagccg gaccatccag cgataaccca acaagaaaat gattttctgc cagaaatgca
     2761 caccattcgt catcaagcag cacgccgtta gtctggaagc tgttacttat cttcctgcca
     2821 gcaccatatt tcgcctgtag cgccacggca cggcggtaaa aatccagccc gagtaacgtt
     2881 ggttcgccgc cctgccaggt aaaagccact tcgttttggg tttcgctggc agcgatatag
     2941 tgacggacat acgcttccag cgtgtcatcg tccatatgcg tgactggctt ttcgcggtaa
     3001 agggattgtt tttcgagata aaaacagtag tcacagttca gattacaatc ggaaccgctc
     3061 ggtttcgcca tcacatgaaa agcacgcgtt ggaacctgtt gcagcatggt cgctccttgc
     3121 ctgaagtcat ccttcaataa gttaagggag aaatcatacc gtggcgcaat gcgttattac
     3181 gggcgtttat tgcgaaggga aagtgacgaa atgcacagaa aacaaaaaac cccgccgaag
     3241 cggggttcaa aattggtcgg cgagagagga ttcgaacctc cgacccactg gtcccaaacc
     3301 agttgcgcta ccaagctgcg ctactcgccg aaatactgct ttttgaattt ttagttcaat
     3361 tctttaaagt cgtggtgcga ggggggggac ttgaaccccc acgtccgtaa ggacactaac
     3421 acctgaagct agcgcgtcta ccaattccgc caccttcgca ttgtcacaac ttctaataat
     3481 ggggtggcta atgggattcg aacccacgac aactggaatc acaatccagg gctctaccaa
     3541 ctgagctata gccaccacta caaatcttgt tacgcggtat tactaccacc gcagctcaag
     3601 cgccgggact aaatggcgcg cccgacagga ttcgaacctg agacctctgc ctccggaggg
     3661 cagcgctcta tccagctgag ctacgggcgc ttagcgccgt tgcgggggtg gataatacgg
     3721 acttcccacc cctctgtcta gtcccttttt aaataaattg cgcgtttggt tacggtttgt
     3781 gcgctttgct gcttatttct ccagtttatg cgctttaccg tggcgattaa gcccaaaaac
     3841 tttataaatc gccgtcaccg tcagcataaa gatgatgccg acaaacagcg acatacgcgt
     3901 atcttcatta aagtacatgc cgatcaaaac gcaaatcagg aatgccattg ttacgtagtt
     3961 ggcccacggg aacaggattg agcggaacgg atggctggca attgccgctt tatgcgcacg
     4021 acgaaaacgc agctggctaa tcaatatcac aaaccatggc accatccccg gaagcacgct
     4081 ggcactgtag acgtagacaa acacacgctg cggattggga atgatgtagt tcaggcatga
     4141 gccaattaac aggatagcaa tagatactgc cacacccgca accggtacgc cgtgacggga
     4201 aactttcgcc atcgccgtcg gtaactgacg gtttttcgcc aatgcgtaga gcatacgtcc
     4261 gcaactgtac atgccgctgt tacagccaga gagcgcagcc gtcagcacca caaagttgat
     4321 aatgcccgcc gctgcggtaa tgccgatttt ggcgaaagtc agtacgaacg ggctgccgtt
     4381 actgcctatt tcattccacg ggaagatggt gacgataacg aaaatcgcgc ctacgtagaa
     4441 aatcaggata cgccacaaca ctttgcctac ggcactgcgc agcgtcacct gcggattctt
     4501 cgcttcaccg gcagtaatgc caatcagctc cacgccctgg taggacgcca ccacaataca
     4561 caatgcggtc aggaaccctt tccagccacc cgcaaagaaa ccgccatgct ctgtgagatt
     4621 gctaaaacca atcgactgcc cgccattgcc aaaaccaaag aaaatcacgc ccaggccaat
     4681 gacaatcatc acgataatcg tggtgacttt gatcatcgcg aaccagaact cgatttcgcc
     4741 gtacaaccgc actgccgcca gattcgccag cgccaccagc gccaccgcga tcaatgcggg
     4801 tatccactgc gccatctccg ggaaccagaa ctggacataa acgccaatgg cggtgatttc
     4861 tgagatcccc accgccatcc acataaacca gtaagaccag gcggtgagat agccaaaaaa
     4921 cgggctcata taacgatgcg cataaacggc gaacgaaccg gtaaccggtt cgaggaacaa
     4981 catttcgccc attgaacgca tgatgaaaaa gacgaacagc ccggcgatga tataggccaa
     5041 caatacggat ggcccggccc atttcagggt actggcggcc cccataaaca ggccgacgcc
     5101 aatggtgccc cccagggcga tgagttcgat atgtcgagct tccagcccac gctgtagctc
     5161 tggtttgtta tctgccataa atcctcgtgt tgtgtttgca tgctttccgg tgttaccggt
     5221 tatcgttatg ggtacatcga gtgttgcgaa tgttttcgta attcaggaga aatggcaaat
     5281 aaagcattaa aaatttgaat gctttgtgta ataaaaaagc agacaggcga cggagtgacc
     5341 actccgtcgc tttacaaaga gaggaaaatc agaggttgcc ggtgtagtgc cagcgtaaat
     5401 aacgcagcaa acgaagctga cgcttaatgc ggctcggctg cgaaagcagg cggtagagcc
     5461 actccagccc cagcgtttgc cagattttcg gtgcgcgttt tacgtgaccg gtgaaaacat
     5521 cgtaggtccc gccaacgccc atatacagcg catctggatg caccagacgg cagtcgcgca
     5581 tgaagatctc ctgctttggc gatcccatcg caacggtgac gatttgcgct ccgctggcat
     5641 gaatgcgttc aaacagcgcc tgacgctgct cgggtttaaa ataaccatcc tgactgccaa
     5701 cgatattcac attccactgg ttgcgcagtt tagcttcagt ttgcgccagc acttcaggtt
     5761 taccgcccac aagaaatacc ggcgtccctt ctttgcctgc gcgcgccatc agctcttccc
     5821 agagatcggc accggcaacg cgggaaacct gcgcctgcgg gtacttttta cgtactgaac
     5881 gtacaacgct gatgccatcc gcatatttaa attcggcagc gttaattagc tccctgacct
     5941 cagcattatc ttcaatggtc agcatttttt cggcattaat ggcaaccagc gttccctgct
     6001 taagctgccc gtcagcaaac agataatcga gggcgtgctg catatcacgc caaccaatca
     6061 actgtaagcc acgcagcgta taggtcggtg ccgtggtgtt gttattcatt gttatccttc
     6121 aacctgcgtc cggagcgatg attttgtacg tttatgaatg agtccggcgc tttcaaaaag
     6181 ccagtacaac agttttgcga tcatcagaca tgcgccgaag accacgataa aaaagactac
     6241 gcgtgagaca aacgaatcca gcccttcacg tgccagcacg atcatattga agatcgcccc
     6301 gaagcagaaa ctgtgcaata tcgcagcctt atagcgatta gtctcgcggt tgcccagctc
     6361 atacagccag tcgaaccatt tgatgatcag accaaccaca atcgccccga gcgggatgaa
     6421 caacgcgccg cccatcacca ccagtgagcc tataagcgta ggcgagatcg ccagcccgga
     6481 gtggttattc agcacttccc aggtaaagta gttggcggag ttcagtacca tactcgggcg
     6541 acccggccac agccaggaag ggataaagac atagaaatcg cggacaatgg gagccaggcc
     6601 ctggaagtcg atgttgtcgt agttctgcaa cagcaacgcc agattctccc acggcgaaaa
     6661 ggtatcgcgg gtgagataga gaaacgtata gaacgcttca tcgccgctca cattcattcc
     6721 ataacgtttt agcgccagcc agaacatgcc cacaatcccc agcacgcccg ccgccgccag
     6781 catccacaac gaaatccagc cgcgaataat gccaataaac aggaagatag cgaatgcgat
     6841 gatgatattg gcgcgagtgc cgccgacaat catgtaagtc aacaagccaa aggcaaccgt
     6901 gctgacgagg aaaaacagcc acgctttgct atcctggcgc agaaaataaa ccaccagcat
     6961 cgccgggatg aaaaagtaaa agaagcgttt taatgccacg ccggagactt cgctggaaaa
     7021 gatctgactg taagaattaa gccggaacag caaaaagccg ttgtgcatga agaagatgcc
     7081 gacgctcacc agagcgatgc ccatcaggat cacccacgtg agattggtct ccacgcggtt
     7141 catggtaaac agcggacggc gcggtgcatc agcaacgcgt ttgcgtagac gggttttgta
     7201 ggtgacatag taaaccgcgt agaagcagcc tgcagaaagc aacgcctgca acaagatttc
     7261 tggcggcgcg acaccaacat caaagcgaaa taccagcacg ctggtcagcg gaaagccgaa
     7321 gaaaaaggtg agcaaaaaca gcaatgaaaa gaagacattg aagttaaagc gcacacggcg
     7381 aaactcaaac caggtcagcg tggcaataaa caacgtgcag agcagccaga caacaaacag
     7441 gccactgaat tgcagcagac tcatgcgacc tccccggcgg caatcgccaa cgcccgctgc
     7501 cagccttgta gatagttagg gctaaagaag gcaatggtgt ttttatccac cgacgccaac
     7561 tggcgctgcg cttcacgcac aatatcctcg ttgagatcat cggtagtaaa cagtactggc
     7621 aaatgctgtt ccgtcatatc ctgccagaac ggattttccc ggttaagcac acaaggaatg
     7681 cccgcctgaa tcagtaagca aagcgtacca atgccctgct ggcgggcaaa aataaagtaa
     7741 ccgagatcgc actgacgaag tagcgtcaga taggcgtcaa attccagttt ttcgctcaga
     7801 acttgcagat tttcttcgct gaataactcc agtcccgcct gacgaacttc ctcgatgtac
     7861 gcttcgttat taggcggata tcccatcggc accaccactt ttaccgtatc gccaaattgc
     7921 tgatgaacgg cgcgcaaggc agcaatatgc tcattgctgc ggtcaccgga gttccccacc
     7981 agaatggtca tttttacttc acgttgccga tcgttcacca tcgtattaag agaagagtcc
     8041 atccgcgtcg ggaagtacag cagttcgccc cgcacctttg ggtgcgtttt ggcaaaaaag
     8101 ctcaaatcac cgcgggtggc aaatacacag ccgactcgct tttgcgccag gcgacgtagt
     8161 gggtaaaaaa gcttatatct caagccactg gaaagttcgt acaggtctgc cccccagata
     8221 tgccagtaaa actggctggg cttaatgcca ccactcagca gagccagcca cagtgtggga
     8281 ttgaactgac cgtggaagaa aaaacgctgc tgacggttag cttttgcttt cgcgatgacc
     8341 gcttccgcca gcgatttttt cccagggaaa aattgcacag aaagcgccag acagctatca
     8401 cttaagccgt cgtccttgcc aacaaccata aactcgcgcg cgtgctcgct cgtcgcggcc
     8461 agcgcgtcgt tgaaaaaccg cagaacggtt cggttatggt gagggatatc cgatcccagt
     8521 acgtgaatca gtacagtcat gcccgcctac gccagagtaa aaacacgcca caacaaagag
     8581 aaaaatagac gatataggtt gccatatagg cctgcgctgc gcccagtgca ccatgcgcag
     8641 ggattagcca gtgggcaaat accatcaata aagtgaactg gctgacttcc gccagaatat
     8701 aaaaccgcag tgacgctttg gcgatcacca gataaccaaa gacataagcg cccactttta
     8761 acacatcacc cactaactgc caggcaaaga gatcgcgcat agcggtaaat ttattcgaca
     8821 acagcagcca gatagcaaaa tcacgcagca accagacggt aaaactcgcc gccgccactg
     8881 ccggtaagac gaattttagc gatttaacca cttcccgggt gatatcgcgc ttttccgtta
     8941 gccgcgacaa cgtgggcagc aaatatacgc tgaacgatgc cgtaataaat tgcaggtagg
     9001 catcggaaat actgctcacc ccttgccaga tccccacctc atcccagcta tactgcgccg
     9061 ccagcagttt acgcatcatg atgtaagcaa caggcaaggt caccgacgta atcaacgcca
     9121 tgagcgtaaa tttgctcaac tgccctgcca gaccgttatc ccagctgggt ttcagatagc
     9181 ttaacgggat gacaccacgt ttgatcaaca tgatggcggc aggaattacc accagcgcgg
     9241 gaatcagcgc cagacccagc aacgcccctt cataaccgcc caaacggtaa ctgacgtagt
     9301 acgcaagaac gccaatcaag ctgccaacaa tcagcgataa cgcattacct gcggcatcgc
     9361 gaaagccttt catcaacgcc agtaacaggt tgccccaggc gatccccatt tgcaccaacg
     9421 ccactaaacg caccagctcc tgatagtcgg tattaccaaa caatccctgg ctgattggcg
     9481 cagctgccag cacaaaaacc agcgccatca gcgtagagaa accaagtacc atcgctgatg
     9541 aagtgccgac cacgcggcgc agctgttggg gattatcatg gtactgggca acgtatttgg
     9601 ttacgccgtt aaagatgcct gccccggcaa gcacgccgag cacggtaatc aactggcgga
     9661 aatttgccgc cagcccaagc cccgccggac caaatgacac tgccagcaac ttaccgacca
     9721 gtaacccggc accaatcttg accagtgtac tggccgcagt ccacaaggac gcttttgcca
     9781 acgacatatc aggagaagta gttcaacaag gtcgcaatta ccgtacgctg attgacgggc
     9841 gacaggttgt agaacaacgg caggcgcagc aggcgctcgc tctctttggt ggtgtagcga
     9901 tcttcaccgt ggaactcacc aaagcgttcc cccgcagggc aaccgtgcag cggaatgtaa
     9961 tggaacaccg ccatgatttc cgcttctttc agaaagttaa tcaacgcgct ccggtcatca
    10021 atatcccgca gtttaatgta gaacatatgc gcgttctgca cgcagccatc gggaatcgac
    10081 ggcagctcga tacgcccggc tttcgccaga ggtgctaacg catcgtagta gttttgccac
    10141 agcgccagac gttgctgatt gatacgatcc gctgcttcca gttgcgccca caggtacgca
    10201 gcttgcaaat cggacatcaa atagctggag ccgatatcgc gccaggtata tttatcgacc
    10261 tgaccacgga agaactggct gcggttagtg cctttttcac ggatgatctc ggctcgttcg
    10321 atcaacgctt tatcgttaat cagcgtcgca ccgccttcac cgcccgccgt gtagtttttg
    10381 gtttcatgga agctaaagca gccaatatga ccaatggttc ccagtgcacg ccctttgtaa
    10441 gtggacataa cgccctgagc ggcatcttcc accacaaaca gattatactt tttcgccaac
    10501 gccataatgg tgtccatttc gcaggccaca cccgcgtaat ggaccggcac gataacgcgc
    10561 gttttgtcgg tgatcgccgc ttcaatcagc gtttcgtcga tgttcatagt gtccgggcga
    10621 acatccacaa agacgatttt cgcgccacgc agcacaaagg cattggcggt ggagacaaag
    10681 gtgtagctcg gcatgatcac ttcatcgcca ggctggatat cgagcagcag cgccgccatc
    10741 tccagagaag cggtgcagga cggcgtcaac agcactttgg cgctgccaaa acgttgttcc
    10801 agccactgct ggcagcgacg ggtaaaaccg ccatcgccac acagtttgcc gctacccatt
    10861 gccgactgca tatagtcgag ttcggttccc accaccggcg gtgcgttaaa tggaatcatg
    10921 tgatcacctg tataaccagt acgcggtgct ttctacattc gcaccacttt gtatgtatcg
    10981 tttaagcgcg gcggtgttgc ccatctgggt cgccacccgc aaagttgttt taccgcgagc
    11041 atacgcccag tttagcgctg tttgcatcag ctcagcacct gcaccgcgtc cagccagcag
    11101 gccaattcgc gcatctgtcg cattgagttc ccgtaaagag acatagccgc gaatatcgcc
    11161 ggacgccgaa cgtaaaatga ggcattgatg atcaaaggtg ccgcgcacgg cattttcaat
    11221 ccactgtgca taaaagcgac cgctggcgtc aggcgcatac cacggcgcac gaaaacggct
    11281 ttgcgcaaat gcggcgctgg ctaactgacg taatgcggga atatcggtct cttgtgccac
    11341 tacagcacca ctatcactga cattgtttac gggtagcgcc aaatcaactt caccttctac
    11401 cagggagaat cccagctgtt gcaaggcatc cagttcatcc gtatttgatg ccgcaatttt
    11461 ggcctgcacc cgtgaccacg gcgctaaggc gtctggcgtc aggagcggtg cttcaggact
    11521 aatgcgcaca atggcgctgt taacaccaaa gaaggcgttt tcccaggtta gtggctcaat
    11581 actggcgcgg acgggcacga agtaactcca gcagatattg gccgtagcca gttttcgcca
    11641 gcgagctggc ggcgcgcttc acgccttcgt catcaagcca gccgttacgc caggcgatct
    11701 cttccaggca ggcaatctta aagccctggc gtttttccac cgtctgcaca aaggtgctgg
    11761 cttcaatcag gctgtcgtga gtgccagtgt ccagccaggc aaatccgcgc ccgagcagtt
    11821 caacggtcag gttgcccgcc tcgaggtaca tctggttgat ggaggtaatc tccagttcac
    11881 cacgctccga cggcttcacc tgctttgcgt actccacgac tttactgtcg tagaaataaa
    11941 gcccggtcac cgcccagttt gacttcggct gttttggctt ttcttccagc gagatagcgc
    12001 ggaaattgtc gtcaaactcc acgacgccaa agcgttccgg atccatcacc tgatagccaa
    12061 ataccgtcgc gccttgcgtg cgcgccgcaa catgacgcag cttcggactg aagccctgtc
    12121 cgaagaagat gttatcgccc agcaccagac aagaaggttc accgttgagg aaggtttcac
    12181 cgatgataaa ggcctgcgcc agaccgtccg gactgggctg ttcggcatat tccagctgga
    12241 taccgaactc actaccatcg cccagcaggc gctggaaata acctttatct tccggcgtag
    12301 tgatgatgag aatttcgcgg ataccggcca gcatcagcac cgacagcgga tagtaaatca
    12361 ttggcttatc gtaaatcggc aacagttgct tcgatacgcc gcgcgtaatc ggatgcaatc
    12421 gggtgccgga accgcccgcc aggataatac ctttcatgtt tgcctccgct gaaaattagc
    12481 ctttcagacc taaacgctcg ccctgatagc tgccgtcctg cacctgcttc caccagcttt
    12541 cattagccag ataccactga accgttttac gcattccact ttcaaaggtt tcctgcggca
    12601 gccagccaag ttcacgggca attttcgaag catcaatggc ataacgcaga tcatgccccg
    12661 gacggtcagc gacaaaggtg atcaggtcac gatagtgcgc cacgccgtgc ggcttgttcg
    12721 gagccagttc ttccagcagc tcgcaaatgg tttccacaac atcgagattc ttacgctcgt
    12781 tgtgaccacc aatattatag gtttcaccga ctttcccggt ggtcgccacg caatacagcg
    12841 cgcgggcgtg atcttccaca tacagccagt cacggatttg ctgcccgttg ccatataccg
    12901 gcagcggttt acccgccagc gcgttgagga tcatcagcgg gatcagtttt tccggaaagt
    12961 ggtaagggcc gtagttattc gagcagttgg tgataagcgt cggcagacca taggtccgca
    13021 gccaggcgcg caccagatgg tcgctgctgg cttttgacac ggaataaggg ctgctcggcg
    13081 catacggcgt ggtttcggtg aagaaatcat ccgtcgagtg caggtcacca tatacttcgt
    13141 cggtagagat atgatgaaaa cggaacgctg attttttatc ttccgtcagc gcattccagt
    13201 aagcccgcgc cgcttcaagc aatgtataag tcccgacaat gttggtttca acaaacgctg
    13261 ccgggccgtc aatagaacgg tcaacatggc tttcggctgc cagatgcatg acacagtctg
    13321 gctgatgctc agtgaatacg cgcgccagtt ctgcccgatc gcagatatca actttctcaa
    13381 aggcaaagcg ctcgctttgc gcgaccggtg ccagcgacat cagatttccg gcgtaggtca
    13441 gcttatcgac cactaccacc gcgtcgctcg tttcgttgat gatataacgc accagcgccg
    13501 agccaataaa cccggcacca cctgttatca gaatttttct catcagcgcc agactccttt
    13561 ggcatcgacg acatactgct gatggacatt gtcgccattg ataactttga actgactatg
    13621 atcgaccagc atcaccagca cgtctgccgt tgccagcgcc tcgtcaagct gcgccagtgt
    13681 acaaagcccg gtcagttttt tcggcaactg gtggatgtta ggctcaacaa ccagagtttc
    13741 gccgctatgc cactgggcga tcagttcagc gatttccatc gctgggcttt cgcgcaggtc
    13801 atcaatattc ggtttaaacg ccagaccaaa gcaggcgatt ttcagttcac tggcgcgttt
    13861 atcggtagca gcaaggcagt cggcaaccgc cgctttcacc tgatcgataa cccagaacgg
    13921 tttgtgatcg ttcacttcgc gcgcggtacg gataagccgc gcctgctgcg ggttctgtgc
    13981 cacgataaac cacggatcaa cagcaatgca gtgaccgccc acgccaggac caggctgaag
    14041 aatattaacg cgagggtgac gattcgccag gcgaatcagt tcccagacgt taattccctg
    14101 atcggcacaa atcagcgaca gttcgttagc aaaagcgata ttcacatcgc ggaagctgtt
    14161 ttcggtgagc ttacacattt ccgccgtccg cgagttagtg acgacacact caccttcgag
    14221 gaaaattttg tacagttcgc tggcgcgggc cgaacaaacc ggcgtcatac caccaatcac
    14281 gcgatcgttt ttaatcagct cgaccataac ctggcccggt aacacgcgtt ccgggcagta
    14341 agcaatgttg acgtccgcca actcgcccac ctgctgtggg aaagtgagat ctggacgcat
    14401 ctctgctaac cattctgcca tcttctcggt tgaccccacc ggcgaggtgg attcaaggat
    14461 caccagcgcg ccttttttca gcactggcgc aatggagcga gcagccgatt caacgtaggt
    14521 catatctggc tcatgatcgc ctttaaacgg cgtgggtaca gcaatcagcc aggcatccgc
    14581 ttcaactggc gtcgtgctcg ctcgtaaaaa accgccttct acggcagttt ttactacgct
    14641 cgccaaatca ggttcgacga tatggatttc gccacgattg atggtatcaa ccgcgtgttg
    14701 gttgatatcg acaccaatca cctgtttttg ccgtgaggca aacgcagctg ctgtaggcag
    14761 cccgatgtaa cccagtccga taacagaaat ggtcgcaaaa ctcatagtga tatccgatta
    14821 ttttttaacg cttccagaat gcgagagcat gcctgaccat caccatacgg gttatgggcg
    14881 cggctcatag cttgatattc gttttcgtct tttaaaagac gcgtcacttc ctcgacaatt
    14941 cgctgcttat ccgtgcctac cagacgcacc gtacccgccg tcaccgcttc cggacgctca
    15001 gtggtatcgc gcattaccag caccggtttc cccagcgaag gcgcttcttc ctgaatgccg
    15061 cctgagtcgg tcaaaatcag ccaggcgtgg ttcatcagcc agacaaacgg taaatactcc
    15121 tggggatcga tcagaatgac atttttcacg tgccccagaa tgcgattgac cggctctctg
    15181 acgttcgggt tgagatgcac tggatagaca atctggatgt cctggtgcgt ggtggcgatg
    15241 tctgccagcg catggcagat ttcttcaaag ccacgaccga agctctcacg cctgtgaccg
    15301 gtcaccagaa tcattttttt attggggtcg ataaacgggt aatttgccgc cagttctgaa
    15361 cgcagcgtgt cgctgctcat cacctggtca cgcacccata acagtgcatc aatgactgta
    15421 ttaccagtaa tgaagattcg gctatccgca acgttttcac gcagcaagtt ttgccgggaa
    15481 gtttcggttg gagagaagtg atacatcgcc agatgcccgg tcaatgtacg gttagcctct
    15541 tccggccacg gcgaatagag atcgcccgtg cgcagaccag cctcaacgtg accaacagga
    15601 atacgctgat aaaacgccgc caggctggtt gccagcgtcg tcgtagtatc gccgtgaacc
    15661 agcacgacgt ctggtttgaa ctcggcaaga ataggtttta gcccttccag aatccgacag
    15721 gttatctctg taaggccctg tcctggctgc attatgttga gatcgtagtc aggtacaatg
    15781 gaaaagagtt tcagcacctg atcgagcatc tcccgatgct gcgcagtgac gcaaacttta
    15841 gcctcaaaaa aaggatcttt tgccaacgca tgcaccaacg gcgccatctt gatggcttcc
    15901 gggcgcgtac caaatacagt cagtactttc acatcgattc tcttcgaata agcggcgagc
    15961 gcctttgcgc tcaccgcagc agtgttgcta tttcgagcaa cggcgggtta atgcgacacc
    16021 agccccgatc agtcccccga caatgcccca cataatcatc aggaaggcac gacgtgggct
    16081 atcgcgtttt accggttctt ccggcgtacg aaaatagcga taggtctgaa aacgcggatc
    16141 cagggttgga ccaacattca gggtgtttaa catggcccga ttctgatcat agtcgagatc
    16201 aaaggccgga ccgacggcct gtaaattttc cagtcgagcc tggagcattg gacgcccaag
    16261 caggaacatt tctgaatcag gtaattcctc ggcaggcaca tctgtcgcac tgcgcgaaat
    16321 attatgctgc tcagcaattt tcagcgcctg ttcaatactg ttcatccggc gatcgtagat
    16381 ggctttcgcc acctcttcct gacgcttcac ctgagctttc atctggatgg tgcgtgccgc
    16441 ccatgcgcct ttcagctcat cattcagatg gctggctgca cgctggctgg caaaagcaac
    16501 atactgacgt aacaggttat tagcgtcagg cgcagtttcg gcaatcagct tcacgctgtc
    16561 attgaccgcg cgggtgaagt caccgggaat aaacacgatg ttgttgatca tttcatccag
    16621 caacgccgca tcggctttgc tgttgcccac catccgctgt ttgtaatagt cggtttgcag
    16681 ccagaactct ctgcgggtat cccacgaggc cagttgcata acaaactctt tataagcttc
    16741 gtccatgacc gatggttggt cggcagaagc catgtttgaa cggacatcca ggttacgcaa
    16801 aaattgctgc tgtgagtaat atccccccag catattcacc gttggacgat cggtaatcgc
    16861 tgtcgagctc cactcctggc gagcaaaaaa agtatacgcc agcgcgatta acgcaaacgc
    16921 cagccccatg ccaataatcc acagcttccc agcccacaag gtacgaaaca acccacgaat
    16981 atccagttca ttttcagcgt cttcggccgg tttcccaggc attggttgtg tcatcacatc
    17041 ctcatttatt tggttaaatt ggggctgcca ccacgatttc tacgcagtct gcgttttacg
    17101 cgcttaataa agcgagcaac tttccaggca cgcttgatgc aatatccata gaggaagaat
    17161 gctagcaaaa agagcaccag catgacccac tccgggacaa aatgagaata ttctgccagc
    17221 acgccaatgg aagcgagcag tgctgcggca agggtaatca gcacaaacgc ctggcgggaa
    17281 gtaaacccgg cacgcatgat caaatggtga atatgctgac ggtctggaga gaatgggctc
    17341 atgcctttac gcaggcgacg gtacataatc gccaccatat ccattagcgg aatggcgatt
    17401 atccacaaag cggtaaccgg gctgatggga tgagttttgc cctgggtcgt ttcgagcagg
    17461 atccagataa cggtaaaacc aatcagcgta ctgcccgcat cccccataaa gactttgtag
    17521 cggcgaccca ggataccaag gttaagcatg atgtatggca ggatggcggc gatcatcgca
    17581 aagcaccaga ttgcgagact ggtttgcccg tcgaaccaca aaatcatacc gattgctgca
    17641 aacgagacgc aggacaaccc acccagcaag ccatcaatgc catcaaccat gttgaacgca
    17701 ttaatggccg cccagacggc aaatagcgtc aggaagtaac caaacggtcc gagcaccatc
    17761 tcccaggagc caaagatata acctaggcta ctgagataaa gtttgccgaa caccatcata
    17821 acaatgccaa cagcggcctg tatggtggca cggattttta cgctgatatc aaaacggtca
    17881 tccagcgcgc caatgaaaac aagcacacca gcacaagcga gatagagaga tgcatgcgga
    17941 atatagtaat cgacaattcc gaacgtgaag caaatccctg cgtaaaccga aatcccccca
    18001 acgagaggta tcaatccctg gtgacgtttg cggaagttag gtttatccac taaaccgact
    18061 ttttttgcca ccttacgggc aaaaaacaga aacagtgtcg tgaataaaaa aatactgatg
    18121 agatcagtac tcactgtcag taaattcaca atgcatgctc tcagagaaaa ttattagcag
    18181 aagtataacc acgaagacct ttattcagaa gggaaagtcc gttccgaacc caccagtcct
    18241 gtgcaaaaat taaccatttg atatgcaaat tgcataacac accagcaaaa tccaggaatt
    18301 ttcctaacat tgccagcgcg gcattaagat tacagatata aaagcaaaac gccacgtaaa
    18361 cacgtggcgt ttttggcata agacaaattt atgagcgttt catcatttcg aagaaatcgt
    18421 cattggtctt ggtcattgcc agtttattaa tgaggaattc cattgcatcg atttcgccca
    18481 tcgggtgaat gattttgcgc aggatccaca ttttctgcag ttcttcctga gtagtgagca
    18541 gctcttcttt acgggtaccg gaacggttgt agtcgattgc cgggaagacg cgtttttcag
    18601 cgatcttacg agagaggtgc agttccatgt tgcctgtacc tttaaactct tcgtagataa
    18661 cttcgtccat cttggaaccg gtatcgataa gcgcggtagc gatgatggtc aggctgccgc
    18721 cctcttccac gttacgcgcc gcaccgaaga agcgtttcgg acgatgcagg gcgttggcat
    18781 ccacaccacc ggtcagcact ttacctgacg ccggaacaac ggtgttgtaa gcgcgcgcca
    18841 gacgagtgat ggagtcgagc aggatgataa cgtctttctt gtgctcaaca aggcgtttcg
    18901 ccttctcgat caccatttcc gcgacctgaa cgtggcgaga tgctggttcg tcaaaggtag
    18961 aagcaacaac ttcacctttt accagacgct gcatctcggt tacttcttcc ggacgttcgt
    19021 cgatcagcag aaccatcagc acacaatccg ggtggttgta agcaatgctc tgagcaatgt
    19081 tctgcagcag catggtttta ccggctttcg gcggtgccac aatcagacca cgctgaccac
    19141 gaccgatagg tgatgccaga tccagtacgc gagcggttaa atcttcagta gaaccgttac
    19201 cacgttccat acgcagacga gagtttgcgt gcagcggggt taagttctca aagaggattt
    19261 tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt aactttcagc agcgcaaaat
    19321 agcgttcacc ttctttcggc gggcgaatct taccagagat ggtatcacca gtgcggaggt
    19381 tgaaacggcg gatttggcta ggggaaacgt agatgtcatc aggaccggcg aggtaggagc
    19441 tgtctgcgga acggaggaaa ccaaatccat cctgcaatat ctccagtaca ccatcaccaa
    19501 agatatcttc gccactcttt gcgtgctgct tcaggatggc aaaaataatg tcctgcttac
    19561 gcatacgagc caggttttcc agccccatat tttcgccgag agtgatcagc tcagaaaccg
    19621 gcgtattctt taattcggta agattcatag tggtgtgagt tcttaaactt ggggtaaatc
    19681 tcgaacttaa tgttgtgaat ggtatggcag gatcatccat gcctgtttac ggtcatcgac
    19741 tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga actgaaacga caagacggat
    19801 tgagtgacga gccagaaatc tgttcacttc gcatttaaga taaaacggga agcgttggaa
    19861 attacaagat tcaaacttaa taaggtatgt ttaatacgaa gtcaacacta agttagcatg
    19921 actcacgccg ggcgtccagt ttttagcgac ggggcacccg aacatgaaat tcccttacgc
    19981 caggttagcg tcgaggaact ctttcaactg acctttagac agtgcgccca ctttggttgc
    20041 cgccacttca ccgtttttga acagcagcaa agtcgggata ccacggatgc catatttcgg
    20101 cgcagtgcca gggttttgat cgatgttcag ttttgcaacg gtcagtttgc cctgatattc
    20161 gtcagcgatt tcatccagaa tcggggcgat cattttgcac ggaccgcacc actctgccca
    20221 gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac tgtcgtcagt
    20281 caggtgaata attttatcgc tcatatataa ctccacagga ataagcctgg cgtgttggtt
    20341 tcgttgttgg tgtaacatta accaactaaa ggttgacttt atttcaccgg atacgctttc
    20401 gtaaagcaat agtaagctga tattctacca cactatgagc aaaacacatt taacagaaca
    20461 gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa gcccttgaaa aaaaagggtt
    20521 tcataactgt acgcccattc aggcactggc ccttccgctg acgctggcgg gtcgtgacgt
    20581 agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg tttcttacgt caacgtttca
    20641 ttatcttctc tctcatcccg cgattgccga tcgcaaggtg aatcaaccgc gtgccttaat
    20701 tatggcaccg acgcgtgaac ttgccgtgca gatccacgcc gacgcggagc cacttgcgca
    20761 agctaccggc ctgaagctgg gtctggctta cggtggtgat ggctacgaca aacagctgaa
    20821 agtgctggaa agcggcgttg acattctgat tggcaccacg gggcgtttaa ttgactacgc
    20881 caagcagaac cacattaacc tcggtgccat tcaggtagtg gtactggacg aagccgatcg
    20941 tatgtacgat ctgggcttta ttaaagatat ccgctggctg ttccgccgta tgccgcctgc
    21001 aaaccagcgc ctcaacatgc tgttctccgc cacgctttcg taccgggtac gtgaactggc
    21061 gttcgagcag atgaacaatg ccgaatatat tgaagtggaa ccggaacaga aaacgggcca
    21121 ccgtattaaa gaagagcttt tctacccttc taacgaagaa aaaatgcgtt tgctgcaaac
    21181 gctgatcgaa gaagagtggc cagacagagc gattattttc gccaacacca aacaccgttg
    21241 tgaagagatc tggggccacc tggcggcaga tggtcatcgt gtcggtttgt tgacaggcga
    21301 tgtcgcgcag aaaaaacgtc tgcgtattct tgatgaattt acccgtggtg atttggatat
    21361 tctggttgcc accgatgttg ccgcgcgtgg cctgcatatc ccggctgtga cgcacgtctt
    21421 taactacgat ttacccgatg actgtgaaga ttacgttcac cgtattggtc gtacaggtcg
    21481 cgctggcgca agtggtcatt ccatcagcct ggcatgtgaa gagtatgcat tgaatttgcc
    21541 tgctattgag acctatattg gtcactcaat tccggtaagc aaatacaatc cggacgcatt
    21601 gatgaccgat ctgccaaaac cgctgcgcct cacgcgcccg cgtacaggca atggtccgcg
    21661 tcgtactggt gctccgcgta atcgtcgtcg ttcaggttaa ataaaatgtc atactcaaaa
    21721 tcattcaaac tgtatcaaga tggcaagaga atgaatcccg atgcgcatgc tgtagtaagt
    21781 gatttgggtg aaagaacgca gccaacacag agacagattg aaggatgaag agtatgggtt
    21841 ccacctcgtc gctgtatgca gccattgatc tcggttcgaa tagttttcat atgctggttg
    21901 tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat taaacgcaaa gtgcgtctgg
    21961 ctgctggcct gaatagcgaa aatgccctgt ctaatgaagc aatggagcgc ggttggcaat
    22021 gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc ctcgcaaatt cgcgttgtcg
    22081 ctacggcgac attacgacta gccgtcaatg cgggtgattt tatcgccaaa gcacaggaaa
    22141 tccttggctg cccggtacaa gtcatcagcg gtgaagaaga agcccgcctg atttatcagg
    22201 gggtggccca cacaacaggc ggtgccgatc agcgcctggt ggtggatata ggcggtgcca
    22261 gtactgaact ggtaaccggc acgggtgcac aaaccacctc gttgttcagc ctgtcgatgg
    22321 gctgcgtcac ctggctggaa cgctattttg ccgatcgtaa tctggcgcag gaaaattttg
    22381 atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt tgcagatgaa ttacggtatc
    22441 acggctggaa agtgtgcgtt ggcgcttccg gcaccgtgca ggcgttacag gaaatcatga
    22501 tggcacaggg gatggatgaa cgcattaccc tggaaaagtt gcagcaactg aaacaacgcg
    22561 ccattcattg cggtcggctg gaagagctag agattgacgg gctgacgctg gaacgtgcgt
    22621 tagtgttccc gagtggtctg gcgatcctga tagccatttt taccgaactg aatattcagt
    22681 gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt ctacggtatg ttgcatctta
    22741 ccgtcgagca ggatattcgc agccgtacgc tgcgtaatat tcagcgccgc tttatgatcg
    22801 atattgatca ggcacagcgc gtagccaaag ttgcggctaa cttctacgat caggtggaaa
    22861 atgaatggca tcttgaagca ataagccgcg atttgctcat cagcgcctgt cagcttcatg
    22921 aaatcggcct gagcgttgac ttcaaacaag cgccgcaaca cgctgcttat ctggtacgta
    22981 atctggatct tcccggtttt acccccgcac agaaaaaact gctggcgacg ctactgctca
    23041 accagactaa tccggtcgat ctctcatcgc tgcatcagca aaatgccgta ccaccgcgcg
    23101 tcgcagaaca actctgccgt ttactccgcc tggctatcat ttttgccagc cgtcgccgtg
    23161 acgatctcgt gccagagatg acattacagg ctaaccatga actgttgacc ttgacgcttc
    23221 cgcaaggttg gctaacccaa catccgctgg gtaaagagat tattgatcag gaaagccagt
    23281 ggcagagcta tgtccactgg ccgctggaag tgcattaatg agtaagtgcc ggatgcgatg
    23341 ctgacacatc ttttccggcc ttgattattt ccctcgtttt gccgccatca tcgctttcag
    23401 attcgccaga tggctttgcc ctttctgcat ccgttcttcg gcgctgacca ctttgcgctc
    23461 ctgttcccaa atcaaatcat cctgcggcag ctccagcagg aagcggctcg gctccgggcg
    23521 caccagttct ccgtactgac ggcgttcttt acacagcgta aaggtcaatt ccttctgggc
    23581 gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg atattatctt catcgatgct
    23641 gctctggtgc ggcaaaaacc cctcttccat accgaccatg tagacataag gaaactccag
    23701 ccctttcgac gcgtggagag tcatcagttg cacctgatcc agctcttctt cactctcacc
    23761 acgctccatc atgtcgcgca aagtaaagcg cgtcaccacc tgggtgagcg tcatcggctc
    23821 atccagttcg ctgccttcca gcatctccgt catccagcta aacagttggt tgacgttctt
    23881 catgcgcatt tcggcggctt tcgggctggg cgatgtttcg tacagccagg attcataatc
    23941 catgccgtgg atcagatcac gcaccgcggc aatcggctcc cgctccgcca gacgctggat
    24001 ttctgccaac cagtgagtga agcgggtcaa tgcttcataa ccacgtccgc taagcgtctg
    24061 actcaggccc atatcaaagc tggcggtaaa catgctttta ttgcgcgtca tcgcccattc
    24121 acccaacttt ttcagcgtcg ccgggccaat ctctcgcttc ggcgtgttaa cgatacgcag
    24181 gaacgcacta tcatcgtccg ggttagtcag cacgcgcaga taagccagca agtccttgat
    24241 ttcaggacga gagaaaaacg acgtaccacc agaaattttg tacgggatgc ggttttgcat
    24301 caggaatttt tcaaacaccc gcgactgatg gttaccgcga taaagaatgg cgtaatcttt
    24361 gtactgcgtt ttattgacga agtgatgggc aatcagctcg ccagtaacgc gctcggcttc
    24421 atgttcctcg ttattcgcgc ttaaaacttt tagctctgtg ccgtaaccca gttcggagaa
    24481 cagacgcttt tcaaagacgt gcgggttatt ggcgatcagg atgttcgccg ctttcaaaat
    24541 acgtccggaa gagcggtagt tctgctcaag cttaatcacc ttcagcgccg gaaaatcctg
    24601 actcagcagc accaggtttt gcggacgtgc gccgcgccag gagtagatcg actggtcatc
    24661 gtcacccacc acggtaaagc gcgcgcggct gcccaccagc agtttcacca gctcatactg
    24721 gctggtgttg gtgtcctgat attcgtccac cagcaggtag cgaattttgt tctgccagcg
    24781 ctcgcggact tcttcattgc gttgcaacag caacgttggc aataaaatca gatcatcgaa
    24841 atcgagaacg ttacaggctt tcaggtgtgc atcatacagc ccataacaat gggcaaaaat
    24901 acggtcccgc tcgccctttg cctctgccgc cgcctgcgcc ggtgttttga gatcgttctt
    24961 ccagttggag atggtcgaaa ttagctgttg caggagaact ttgtcatctt caatcagccc
    25021 ctcggtcaac tctttaagca aagcaagctg atcggtatcg tcaaacagcg aaaagttagc
    25081 cttcatcccc aacgccgcgt actcacgctt aatgatatcc agccccagcg tgtgaaaggt
    25141 ggagatcatc agcccacgcg cctctttacg ccccagcgtc tgcccgacac gctctttcat
    25201 ctcgcgtgcc gccttattgg taaaggtcac cgccgcgata tgtcgcgcct gataaccgca
    25261 accgcggatc agatgggcga ttttattggt gataacacga gttttaccgg aacccgcgcc
    25321 cgccagcacc aggcaggggc cggtaacgaa ttcgacagct tgttgttggc cggggtttag
    25381 acgcatagga aaatcgctca atgaaagtcg ggaggggaaa ataacagcgt ggtagtatag
    25441 ccagcctcat ccttaccact caaggcacga tcatggcaaa aacagcagca gcactgcata
    25501 tccttgtaaa agaagagaaa ctggctctgg atcttctcga gcagatcaag aatggtgccg
    25561 atttcggcaa gctggcgaag aaacactcca tttgcccgtc aggcaaacgc ggcggtgatt
    25621 tgggtgaatt ccgccagggt caaatggttc cggcgttcga taaagtggtt ttctcttgtc
    25681 cggtactgga gccaaccggc ccgctgcata cccagttcgg ttaccacatc attaaggtgc
    25741 tgtaccgtaa ctaacagcaa ggccttctcc gggagaaggc cttgaactct gaatcgctat
    25801 taacccgcaa cagcaatacg tttcatatcc gtcatatagc cgcgcagttt cttacctacc
    25861 tgctcaatcg catggctgcg aatcgcttca tttacatcac gcagctgcgc gttatctacc
    25921 gcaccttccg gaatagcttt acccaggtcg cccggttgca gctctgccat aaacggtttc
    25981 agcagcggca cacaagcgta agagaacaga tagttaccgt actcggcagt atcggagata
    26041 accacgttca tttcgtacag acgcttacgg gcgatggtgt tggcaatcag cggcaattcg
    26101 tgcagtgatt catagtaagc agattcttcg atgatgccgg aatcaaccat ggtttcaaac
    26161 gccaactcaa cgcctgcttt taccatcgcg atcatcagta cgcctttatc gaagtactcc
    26221 tgttcgccga ttttgccttc atactgcggc gcggtttcga atgcggtttt gccagtctct
    26281 tcacgccagg tcagcagttt cttatcgtcg ttggcccagt cagccatcat gccggaggag
    26341 aattcgccgg agatgatgtc gtccatatgt ttctggaaca gcggcgccat gatctctttc
    26401 agttgctcag agagcgcgta agcacgcagt ttcgccgggt tagaaagacg gtccatcatc
    26461 agggtgatgc cgccctgttt cagcgcttcg gtgacggttt cccaaccgaa ctgaatcagt
    26521 ttttctgcgt atgccggatc ggtgccttct tccaccagct tgtcgaagca cagcagagaa
    26581 ccagcttgca acataccgca caggatggtt tgctcgccca tcaggtcaga tttcacttcc
    26641 gcaacgaaag aggattccag cacgcccgca cggtgaccgc cggttgcagc cgcccatgct
    26701 ttagcgatcg ccatgccttc gcctttcgga tcgttttccg ggtgaacggc aatcagcgtc
    26761 ggtacgccga atccacgttt gtactcttca cgtacttcgg tgccagggca tttcggcgca
    26821 accattacga cggtgatgtc tttacggatc tgctcgccca cttcgacgat gttgaaaccg
    26881 tgtgagtaac ccagcgccgc gccgtctttc atcagtggct gtacggtgcg tactacatca
    26941 gagtgctgct tgtccggcgt caggttaacc accagatccg cctgcgggat cagttcttcg
    27001 taagtaccca ctttaaaacc attttcggtt gctttacgcc aggatgcgcg cttctcggca
    27061 atcgcttctt tacgcagagc gtaagaaata tccagaccag aatcacgcat gttcaggccc
    27121 tggttcagcc cctgtgcgcc acagccgacg atgactactt ttttaccctg aaggtagctc
    27181 gcgccatcgg cgaattcatc gcgccccata aagcgacatt tgcccagctg tgccagctgc
    27241 tggcgcagat tcagtgtatt gaagtagtta gccatggtga ttcctcgtga tgttgtgctt
    27301 cttattgttc ggttcgcgtt tgcgagattg aattcactat atgacaggaa atttattgcg
    27361 gaaattgata tattcacaac gtcacattgc aatttctgca acgtcaacat tgagggctgc
    27421 gccattggat ttacgcgatc tgaaaacctt cctgcatctg gcggaaagcc gccattttgg
    27481 ccgtagcgcg cgggcaatgc acgttagccc gtctacgctt tcgcgacaaa tacagcgcct
    27541 ggaagaagat ctcggtcagc cgctgtttgt gcgtgataac cgcacggtaa cgctgacgga
    27601 agcgggcgaa gagctgcgcg ttttcgccca gcaaacgctg ttgcagtatc agcaattgcg
    27661 ccacaccatc gaccagcaag ggccgtcgct ctctggcgaa ttacatatct tctgctcggt
    27721 gaccgctgcc tacagccatc tgccgccgat tctggatcgc ttccgcgcgg aacatccgtc
    27781 agtggagatc aaactctcta ccggtgatgc ggccgatgcg atggaaaagg tggtcactgg
    27841 cgaagcggat ctggcgattg ccgggaaacc ggaaaccttg ccgggcgcag tggcgttttc
    27901 gatgctggag aatctggcgg tggtgctgat tgctcccgcg ctgccctgcc cggtgcgtaa
    27961 tcaggtatct gtcgaaaagc ccgactggtc tacagtgccg tttatcatgg ccgatcaggg
    28021 accggtacgt cgccgcattg aactgtggtt tcggcgtaac aaaatcagca acccaatgat
    28081 ttacgccacg gttggcgggc atgaagcgat ggtatcgatg gtggcgctcg gctgtggcgt
    28141 ggcgctgttg ccggaagtgg tgctggataa cagcccggaa ccggtgcgta accgcgtgat
    28201 gattttagag cgcagtgatg aaaaaacgcc gtttgagctg ggcgtgtgcg tacaaaaaaa
    28261 gcggcttcat gagccgctga ttgaggcgtt ctggaagatt ttgccgaacc actaacccgc
    28321 caaaaagaac ctgaacgccg ggttattggt ttcgtcgtgg caatcgtagc ccagttcatt
    28381 cagccgggtt tcaaaatccg gttcatgatc gccaagctcg aacgccgcca gtacgcgccc
    28441 gtagtcggta ccgtggctgc gataatggaa cagcgagatg ttccagtgcg tacccagcgt
    28501 gttgaggaag cgcagcagcg cgcccggcga ttccgggaat tcgaagctgt agaggcgttc
    28561 ctgcaacgga tgcgatggac gcccgccaac catatagcgc acatgcagct tcgccatttc
    28621 gtcgtcggag agatccacca cgctgtagcc gccatcgttg agcatctgca aaatttcttt
    28681 gcgctcttcg aggccacggc tcaggcgcac gccgacaaaa atgcaggcgt ttttggcatc
    28741 agcaaagcgg tagttgaact cggtgaccga gcgcccacca agtaactggc agaatttgag
    28801 gaagctgccc ttttcttccg gaatggtcac cgccagtaac gcttcacgct gttcgcccag
    28861 ttcgcagcgt tccgagacgt agcgcagacc gtgaaagttc acgttggcac cggaaagaat
    28921 atgcgccagc cgctcaccgc gaatgttgtg ctgggcgatg tattttttca tccctgccag
    28981 cgccagcgcg ccagagggtt cagccaccgc gcgtacatct tcgaacaggt ctttcatcgc
    29041 cgcacagatg gcatcgctat cgacggtgat gatgtcgtca agatactcct ggcacaaacg
    29101 gaaggtttca tcgccgatgc gttttaccgc gacgccttca gcaaacagcc ccacgcgggg
    29161 cagatcaacg ggatgacctg catcaagtgc cgctttcagg caggcggaat cttccgcttc
    29221 tacggcgatc actttgattt gtggcatcag ttgtttgatc agcaccgcca cgcccgccgc
    29281 cagaccgccg ccgccgaccg gaacaaatac gcggtcgaga tgagcatcct gctggagcag
    29341 ttccagcgcc agcgtaccct gaccagcgat caccatcgga tgatcgaagg gtggcaccca
    29401 ggtgaagccc tgctgctgtg acagttcgat tgctttcgct ttcgcttcgt caaagttcgc
    29461 gccatgaagc agcacttcgc cgccaaagcc gcgcaccgca tcaactttga tatcggcggt
    29521 ggcggttggc atgacgatca gcgccttcac gcctaaccgt gcggaagaaa acgcgacgcc
    29581 ctgcgcgtgg ttacccgcag aagcggtaat cacgccgtgc gctttttgtt cttccgtcag
    29641 gcccgccatc atggcgtatg caccacgaag cttaaagctg tgcactggct ggcgatcttc
    29701 gcgcttcacc agaatcacgt tatcaagacg cgacgacagt ttttccattt tctgtagcgg
    29761 cgtgacctgc gccgcttcgt agaccggtgc gcgcagcacc gctcttaaat attcggcacc
    29821 ttccggggca ccggacaggg gttgtgagtc tgccatcatt aaccccccag tttcgattta
    29881 tcgcgcaccg cgcctttgtc ggcgctggtt gccaggctgg cgtaggcgcg cagggcaaag
    29941 gagacctgac gttcacggtt tttcggcgtc caggctttgt caccccgagc ttcctgcgct
    30001 tcacgacgcg ccgccagttc ggcatcgctt acctgtaact gaataccacg gttcggaatg
    30061 tcgatggcga tcaggtcgcc gtcttcaatc aggccaatgc tgccgccgct tgccgcttcc
    30121 ggtgagacgt ggccgataga aaggccagag gtaccgccag agaaacgacc gtcggtgatc
    30181 agcgcacagg ctttaccgag acccattgat ttcaggaagc tggttgggta gagcatttcc
    30241 tgcatccccg gaccgccttt cgggccttca tagcgaatta ctaccacatc tccggcgaca
    30301 actttaccgc cgagaatcgc ttctaccgca tcgtcctggc tttcgtacac tttcgccggg
    30361 ccggtgaatt tgaggatgct gtcatccacg cccgcggttt taacgatgca gccgttttcc
    30421 gcaaagttac cgtagagcac cgccaggccg ccgtctttgc tgtaggcgtg ttccagcgag
    30481 cggatacagc cattggcgcg atcgtcgtcc agcgtatccc aacggcaatc ttgcgagaat
    30541 gcctgggtgg tacggatacc cgcagggccg gcgcggaaca tattttttac cgcgtcatcc
    30601 tgggtcagca taacgtcgta ttgttccagc gtttgcggca acgtcaggcc gagcacgttt
    30661 ttcacatcac ggttcagtaa ccccgcgcga tccagttcgc cgagaatacc gataacacca
    30721 ccagcacggt gaacatcttc catatggtat ttctgggtgc tcggcgcaac tttacacagc
    30781 tgtggaacct tgcgggaaag cttatcgata tcactcatgg tgaagtcgat ttccgcttcc
    30841 tgcgccgccg ccagcagatg aagtacggtg ttggtagagc cgcccatcgc gatatccagc
    30901 gtcatggcgt tttcaaacgc cgccttactg gcgatattac gcggcagtgc actatcgtcg
    30961 ttttgctcgt aataacgttt ggtcaattca acaatgcgtt taccagcatt gaggaacagc
    31021 tgcttacggt ccgcgtgggt tgccagcaac gagccgttgc ccggctgcga caggcccagt
    31081 gcttcggtca ggcagttcat tgagttagca gtaaacatcc cggagcagga accgcaggtt
    31141 gggcaggcgg atcgttcaac ctgatcgctc tgggagtcag aaactttcgg gtctgcaccc
    31201 tggatcatcg catcaaccag atcgagctta atgatctgat cggacagttt ggttttcccg
    31261 gcttccatcg ggccgccgga aacaaagatc accggaatat tcaggcgcag ggaagccatc
    31321 agcatccccg gggtgatttt gtcgcagtta gagatgcaga ccatggcgtc ggcgcagtga
    31381 gcgttgacca tatactcaac ggaatcagcg atcaattcgc gagatggcag tgaataaagc
    31441 atccccccgt ggcccatggc aatcccatca tccaccgcaa tggtgttgaa ctctttggca
    31501 acgccgccag ccgcttcaat ttgttcggcg accagtttac cgagatcgcg cagatggacg
    31561 tgacccggta caaattgggt gaacgagttc acaaccgcga taatcggctt accgaaatcg
    31621 gcgtcggtca ttccggtggc gcgccacagc gcacgagcac ccgccatatt acgaccatga
    31681 gtggtggtgg cggaacggta cttaggcata ctttatttac tcccagtgtc tgtctcgtaa
    31741 atgggacggt gcgtgccgtc ccattttttg tatttattga ttaacttgat ctaaccagcc
    31801 ccatttatct tcggtttcgc cagtgaagag gccgaagaag gcttgctgaa tgcgtttggt
    31861 aaccgggccg caacggcctt caccaacctg aataccgtct acactgcgca ctggcgtgat
    31921 ttctgccgca gtaccggaca taaacacttc atccgccagg tacagtgatt cgcgcgacag
    31981 cacctgctca cgcacttcaa ttcccagctc tttcgccagt ttgatgatgg cgtcacgggt
    32041 aatacccggc agcgcggatg aggtgaacgg tggggtgaac agcacaccgt ctttcacttc
    32101 aaacaggttt tcgcctgcgc cttcagagat gtaaccgttc acatccagcg cgataccttc
    32161 ctgataaccg tggcggcgtg cttcactacc caccagcagg gaagagaggt agttaccacc
    32221 ggcttttgcc gcggttggga tggtgtttgg tgctgcgcgg ttccaggagg aaaccatcgc
    32281 atcgatcccc tgctccagcg cttctgcgcc cagatacgcc ccccacggga aagcggcgat
    32341 aatcacgtcg gttgagtatc ccgctggcgg gtttacgccc atgccaacat caccaacgaa
    32401 gatcagcgga cggatatagg cgctggtgag attgtttttg cggatcacgt cacgacaagc
    32461 ttccatcagc tcatcaatgc tctgcgaaac cgggaagcga tagattttgg cggagtcatg
    32521 cagacgctgc atatgctcac gatggcggaa tacaaccggt cctttgtgcg agtcgtagca
    32581 acggatgcct tcaaaaaccg aggtgccata gtgcagcgcg tgcgacatca catgcacctt
    32641 cgcgtcttcc cagcgaacca tctccccatt gaaccaaatg taatcagctt tcttcgtggt
    32701 catttttata ttccttttgc gctcaggcgc ggatttgttg tgatgtggtt gtgctctggc
    32761 agatggcaac gtgtgcgacg tccaccagtt tatttaactg actaaacagt aagtcgaccg
    32821 accgtgggct ggcaacggtc aattcgatat ttatattttg tgcatcgctg gcggcggcca
    32881 tattcattga acagacgtgg aaaccacgat gacgcaccac gcgtaaaaca cgttctaagg
    32941 tttccggatt gaagcgagcc gatacattga cctgatgttg catcatgata atttctccaa
    33001 catttctgaa ttactggcac caggcggcac cagcggccag acgttctcaa gttcgtcgat
    33061 tgagacatga agcaggtatg gcccatcact gttcagcatg gtgtcgagtg ccgcttcaac
    33121 ctggtcttta tgggtgatgt gttggccagg gatgccgaag gcgctggcta acatgaggaa
    33181 atcggggtta tcggtaaggg tggtttcgct gtatcgttcc tggaaaaaca gttgctgcca
    33241 ttgtcgaacc atccctaacc gttggttatc gagtaagacg attttcaacg gtaactgctt
    33301 gcgttttacg gtgcccagct cttgcacatt catcatgaaa gagccgtcac cggagataca
    33361 gacgacagta tcgttcggtc gcgcgacttg tgcgccaacc gccgccggta aaccgaaacc
    33421 catggtgcct aagccgctgg aggtgatgaa gttttccggg cgagtgtgga cgatgtgctg
    33481 ggcagcccac atctggtgct gccccacatc tgtggtcacg atgcaatccg cagatttacg
    33541 atccgacagt tgttttaaca acaacggcgc gtagatagcg tcaccaggat ggtcgtaacg
    33601 ccaggcatgt tcatcacgca gttgcgcgca gtgttgctgc cagtcatcga tatttaacgg
    33661 ctgctgtaat gctggtaaca gagcatttaa atcaccctgt aatgccacat gtgcctgacg
    33721 cagcttgttc atttctgccg gatcgatatc catatggata acgctggcgt gtggtgcgaa
    33781 ggtgttcagt ttgccggtca cccggtcatc aaaacgtgcg cccacggcga tcagtagatc
    33841 gcactcctgc actgcgaagt tcgccgcttt ggtgccgtgc atccccagca tgcccagata
    33901 gtacggataa tctgcttcta ctgcgcccag ccctttcaac gtacaggtgg caggcatttt
    33961 tgtggcagcg agaaattcac gcaaagccga aactgcctgc gccatgccca cgccaccacc
    34021 aacgtacagc atcggttttt gcgcttttgc cagcatctga cgcgcttgct cgacttcggc
    34081 atgtgggaaa gtcacttcgt tttcaacagt ggtgaaccac ggttccagat cgccgctggc
    34141 taactggata tcttttggaa tatcgaccaa aaccggacca ggacgaccag agctggcgac
    34201 gtcaaacgct tcggccatga tacgcggtaa ctcttccagt gattgcacca ggaaactgtg
    34261 cttggtgcag gctaacgaca accccagaat atccacctcc tgaaatgcgt cagtgccgat
    34321 aaacggtgcg gatacctgac cggtgatagc aacaacgggg atggaatcta acagcgcgtc
    34381 cgcaagcccg gttatcaggt tggttgcacc tggaccagac gtggcgatgc aaacgcccgt
    34441 tttgccggta gcacgggcat aaccgatagc cgccattgct gcaccttgct cgtgtcggca
    34501 cagcaagtgt tccacgccac cgtcatacaa tgcatcgtaa accggcataa ttgcgccacc
    34561 cggataaccg aaaacggtgt tcacaccctg tgcccgcaac gcatgtacca cccactgtgc
    34621 gccattcata gttagttccc cgtcctgaat cttgagaaac agaattttgt gctgttattc
    34681 attgtctgct cctcagttat gtttttaagg tcaaaaaaaa cccccggacc tttcggtgcg
    34741 ggggtcttag ttcgttaagg cttgatctct aagcctttcc tcgtccaagt gcagccccgc
    34801 acggtgggat aataatcacc accacgctaa tcacgaccag gctaatcact cgtagaaggg
    34861 ctgtcatttt gtcttttctt gcatcttgtt cgaaggaatg cctaaagagt taccatagat
    34921 tttgcgaata gcacaagata ttttttggcc gatttttatg atgtctttaa taatttcaag
    34981 agaaattgtt gttttttaaa aagaaaatag tgagtgaaaa aaattcaatt tcagtatgaa
    35041 tgttgttccc ttcctcgtag ttcatccttt gcccgctgca tttttgcgag catcattcca
    35101 ccgccagaac aactcaactt cacttgcaca aataaacagg aatcagccac taaaattcac
    35161 gaattagcgg gctacagcag cgatatctta tgcacataat ggtgactcaa ggagggctta
    35221 tgtcactgtc aattgttcat acccgcgcag ccctgggagt aaatgcgccc ccaatcactg
    35281 ttgaggtaca tatcagtaaa ggtctaccag gcttaacgat ggtgggctta ccagaaacaa
    35341 cggtaaaaga agctcgcgat cgcgtgcgca gcgccattat caatagcgga tatgaatatc
    35401 cggcgaaaaa aatcaccatc aaccttgctc cagccgatct gccaaaagaa gggggacgat
    35461 atgatttgcc tatcgctatt gcgttactgg cggcctcaga acagcttaca gccaataagt
    35521 tagatgaata tgaattagtc ggagaactgg cgcttacagg cgctctgcgt ggcgttcccg
    35581 gcgcaatctc cagtgcaact gaagccatta agtcgggcag aaaaattatc gtcgcgaaag
    35641 ataacgagga tgaagtgggg ctaattaacg gtgaaggatg cctgatagcc gatcatctgc
    35701 aggctgtctg tgcgtttctg gagggtaagc acgctctcga acgcccgaaa ccaactgatg
    35761 cagtatcccg ggcgctacaa catgatctca gtgatgttgt cggtcaggag caaggaaagc
    35821 gaggactgga aattaccgcc gctggcgggc acaacctttt actgattggg ccgccgggaa
    35881 caggtaaaac aatgctcgcc agccgtatta atggcctgtt gccagattta agcaatgaag
    35941 aggcactgga gagcgctgcg atattaagtc tggtaaatgc tgaatcagta caaaaacaat
    36001 ggcggcagcg cccattccgc tcacctcatc acagtgcgtc gttaactgcg atggtgggcg
    36061 gtggtgcaat tccagggcca ggtgaaattt cgctggcgca taacggcgtg ctttttcttg
    36121 atgagctacc tgaatttgaa cggcgtacac tggatgcctt gcgagagccg attgaatccg
    36181 ggcagatcca tctttcacgc acacgagcaa aaataaccta tccagcccgt ttccaacttg
    36241 ttgcggcgat gaatcccagc cctaccggac attatcaggg aaaccataac cgctgcacac
    36301 cagaacagac attacgttat ctcaaccggc tctcggggcc ctttctcgac cgcttcgatc
    36361 tctcactaga gatcccatta ccgccccccg gcattttgag taaaacggta gtgcagggag
    36421 aaaacagcac caccgttaaa caacgtgtaa tggccgccag agagcgccaa tttaagcggc
    36481 agaataaact gaacgcctgg ctggataatc cggaaatacg ccaattctgc aagcttgaga
    36541 gcgaagatgc gcagtggctg gaagaaacgc tgatccatct ggggttatcg attcgtgcct
    36601 ggcagcggtt attgaaagtt gcacgaacca ttgctgatat tgatcagtct gacattatca
    36661 cacgtcagca tttgcaggag gcagttagct atcgtgcgat tgaccgtttg ctcatccatc
    36721 tgcaaaaact actgacataa aaaaaagggc atttcgccct ttttattaat cgtcagaatc
    36781 ggtgtagtct tcagcacctt caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt
    36841 tggacgcttg atacgcgtca tatacttgga ccacacgcgt tctgcttctg tcactggctc
    36901 acgttcgcca cggcatactg ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt
    36961 gccaagatcc aactcattga aggcataacc atgacgctca agcagttgtg cctctttgat
    37021 ggtgaagtca ccatgacgag agaatccacg tggataatgt ttattgtcga aatatcgatt
    37081 agtcgtcgta aagctttccg ccatcctgca cgctcctaat tctttgaccg agctagttat
    37141 ggcgcggagt attagttacg cttgacagag tgtaaaacaa aacatttaaa tcataacgac
    37201 aaataatttt gcggagagca ctgtggatac ggaattgtta aaaactttcc tggaagttag
    37261 ccgaacgcgt cactttggtc gagcagctga atcgctctat ctgacccaat cagccgtgag
    37321 ctttcgaatc agacaactgg aaaatcaact gggtgtgaac cttttcaccc gccacagaaa
    37381 caatatccgt ttaaccgctg ctggtgaaaa actactgcct tatgcagaaa cgctcatgag
    37441 cacctggcag gccgcccgta aggaggtggc gcatacctca cgacataacg agttttctat
    37501 cggtgccagc gcctcgttgt gggaatgtat gcttaatcag tggctgggac gcttgtatca
    37561 aaatcaggat gtccatacag gcttacagtt cgaagcgcga attgcccaac ggcagtctct
    37621 ggtaaaacag ctgcatgaac gccagcttga tcttcttatt actactgaag cgcccaaaat
    37681 ggacgaattt agcagtcagt tgctcgggta tttcacttta gcgctttata ccagtgcccc
    37741 ttctaaacta aagggagatc ttaactatct gcgactggaa tgggggccag attttcaaca
    37801 gcatgaggca ggtttgatcg gtgctgacga agttcccatt ctgacaacca gttctgctga
    37861 actggcacag caacagattg cgacgcttaa tggttgcacc tggctacccg tcagttgggc
    37921 gcgtaaaaag ggcggcctgc ataccgttgt cgatagcaca acactttcac ggccgcttta
    37981 tgccatatgg ctgcaaaata gcgataaaaa tgcgttgatt cgcgatctat tgaaaattaa
    38041 cgtgctggat gaagtgtatt aatctgaatg gctggcaagg atgccggtag aaggatttac
    38101 ttcggagagg ttatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctggc
    38161 aggggcggag agactcgaac tcccaacacc cggttttgga gaccggtgct ctaccaattg
    38221 aactacgccc ctaattaggg tggcggaacg gacgggactc gaacccgcga ccccctgcgt
    38281 gacaggcagg tattctaacc gactgaacta ccgctccacc gaattctttt acaaccaccg
    38341 gttttatgac cggctt
//
LOCUS       DAZKGF010000023        36890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZKGF010000023 DAZKGF010000000
VERSION     DAZKGF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.023"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..273)
                     /gene="tssD"
                     /locus_tag="ACHOQV_001192"
     CDS             complement(<1..273)
                     /gene="tssD"
                     /locus_tag="ACHOQV_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFP9629590.1"
                     /translation="MSDIVYLRIIGEKQGYISSGCGTYASVGNCWQVGHEDEIFAFAL
                     TNAITSTGKGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYR"
     gene            799..2172
                     /gene="aroP"
                     /locus_tag="ACHOQV_001193"
     CDS             799..2172
                     /gene="aroP"
                     /locus_tag="ACHOQV_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFP9629591.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTCFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(2215..3069)
                     /gene="ampE"
                     /locus_tag="ACHOQV_001194"
     CDS             complement(2215..3069)
                     /gene="ampE"
                     /locus_tag="ACHOQV_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFP9629592.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQIDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVLVVIAL
                     LTIYGALV"
     gene            complement(3066..3617)
                     /gene="ampD"
                     /locus_tag="ACHOQV_001195"
     CDS             complement(3066..3617)
                     /gene="ampD"
                     /locus_tag="ACHOQV_001195"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFP9629593.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLVAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3705..4598
                     /gene="nadC"
                     /locus_tag="ACHOQV_001196"
     CDS             3705..4598
                     /gene="nadC"
                     /locus_tag="ACHOQV_001196"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFP9629594.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANE
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4801..5241
                     /gene="ppdD"
                     /locus_tag="ACHOQV_001197"
     CDS             4801..5241
                     /gene="ppdD"
                     /locus_tag="ACHOQV_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFP9629595.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            5251..6636
                     /gene="gspE"
                     /locus_tag="ACHOQV_001198"
     CDS             5251..6636
                     /gene="gspE"
                     /locus_tag="ACHOQV_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFP9629596.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVLPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAISFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVENPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAINAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6626..7828
                     /gene="hofC"
                     /locus_tag="ACHOQV_001199"
     CDS             6626..7828
                     /gene="hofC"
                     /locus_tag="ACHOQV_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFP9629597.1"
                     /translation="MASKQLWRWRGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALFPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPVLTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNAGEFSPLCLQLVRTE
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7863..8906)
                     /gene="guaC"
                     /locus_tag="ACHOQV_001200"
     CDS             complement(7863..8906)
                     /gene="guaC"
                     /locus_tag="ACHOQV_001200"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP9629598.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSFEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(9062..9106)
                     /locus_tag="ACHOQV_001201"
     CDS             complement(9062..9106)
                     /locus_tag="ACHOQV_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629599.1"
                     /translation="MIIKTLKMSARKRN"
     gene            9131..9751
                     /gene="coaE"
                     /locus_tag="ACHOQV_001202"
     CDS             9131..9751
                     /gene="coaE"
                     /locus_tag="ACHOQV_001202"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP9629600.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     PREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9751..10494
                     /gene="zapD"
                     /locus_tag="ACHOQV_001203"
     CDS             9751..10494
                     /gene="zapD"
                     /locus_tag="ACHOQV_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFP9629601.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPVVDYAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            10504..10701
                     /gene="yacG"
                     /locus_tag="ACHOQV_001204"
     CDS             10504..10701
                     /gene="yacG"
                     /locus_tag="ACHOQV_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFP9629602.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10903..11301)
                     /gene="mutT"
                     /locus_tag="ACHOQV_001205"
     CDS             complement(10903..11301)
                     /gene="mutT"
                     /locus_tag="ACHOQV_001205"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFP9629603.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHLSLFEILEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVLAKLKRVYVG"
     gene            complement(11361..14066)
                     /gene="secA"
                     /locus_tag="ACHOQV_001206"
     CDS             complement(11361..14066)
                     /gene="secA"
                     /locus_tag="ACHOQV_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP9629604.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(14128..14640)
                     /gene="secM"
                     /locus_tag="ACHOQV_001207"
     CDS             complement(14128..14640)
                     /gene="secM"
                     /locus_tag="ACHOQV_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFP9629605.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14871..15788)
                     /gene="lpxC"
                     /locus_tag="ACHOQV_001208"
     CDS             complement(14871..15788)
                     /gene="lpxC"
                     /locus_tag="ACHOQV_001208"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFP9629606.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15889..17040)
                     /gene="ftsZ"
                     /locus_tag="ACHOQV_001209"
     CDS             complement(15889..17040)
                     /gene="ftsZ"
                     /locus_tag="ACHOQV_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9629607.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(17101..18363)
                     /gene="ftsA"
                     /locus_tag="ACHOQV_001210"
     CDS             complement(17101..18363)
                     /gene="ftsA"
                     /locus_tag="ACHOQV_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP9629608.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(18360..19190)
                     /gene="ftsQ"
                     /locus_tag="ACHOQV_001211"
     CDS             complement(18360..19190)
                     /gene="ftsQ"
                     /locus_tag="ACHOQV_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFP9629609.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(19192..20112)
                     /gene="ddlB"
                     /locus_tag="ACHOQV_001212"
     CDS             complement(19192..20112)
                     /gene="ddlB"
                     /locus_tag="ACHOQV_001212"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9629610.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDANPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(20105..21580)
                     /gene="murC"
                     /locus_tag="ACHOQV_001213"
     CDS             complement(20105..21580)
                     /gene="murC"
                     /locus_tag="ACHOQV_001213"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP9629611.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21634..22701)
                     /gene="murG"
                     /locus_tag="ACHOQV_001214"
     CDS             complement(21634..22701)
                     /gene="murG"
                     /locus_tag="ACHOQV_001214"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9629612.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22698..23942)
                     /gene="ftsW"
                     /locus_tag="ACHOQV_001215"
     CDS             complement(22698..23942)
                     /gene="ftsW"
                     /locus_tag="ACHOQV_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFP9629613.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23942..25258)
                     /gene="murD"
                     /locus_tag="ACHOQV_001216"
     CDS             complement(23942..25258)
                     /gene="murD"
                     /locus_tag="ACHOQV_001216"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP9629614.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAVTGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKICVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(25261..26343)
                     /gene="mraY"
                     /locus_tag="ACHOQV_001217"
     CDS             complement(25261..26343)
                     /gene="mraY"
                     /locus_tag="ACHOQV_001217"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP9629615.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(26337..27695)
                     /gene="murF"
                     /locus_tag="ACHOQV_001218"
     CDS             complement(26337..27695)
                     /gene="murF"
                     /locus_tag="ACHOQV_001218"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP9629616.1"
                     /translation="MISVTLSQLTDILNGELKGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVNRPLDIDLPQVIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHIQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27692..29179)
                     /gene="murE"
                     /locus_tag="ACHOQV_001219"
     CDS             complement(27692..29179)
                     /gene="murE"
                     /locus_tag="ACHOQV_001219"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFP9629617.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(29166..30932)
                     /gene="ftsI"
                     /locus_tag="ACHOQV_001220"
     CDS             complement(29166..30932)
                     /gene="ftsI"
                     /locus_tag="ACHOQV_001220"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFP9629618.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30948..31313)
                     /gene="ftsL"
                     /locus_tag="ACHOQV_001221"
     CDS             complement(30948..31313)
                     /gene="ftsL"
                     /locus_tag="ACHOQV_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP9629619.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(31310..32251)
                     /gene="rsmH"
                     /locus_tag="ACHOQV_001222"
     CDS             complement(31310..32251)
                     /gene="rsmH"
                     /locus_tag="ACHOQV_001222"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP9629620.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(32253..32711)
                     /gene="mraZ"
                     /locus_tag="ACHOQV_001223"
     CDS             complement(32253..32711)
                     /gene="mraZ"
                     /locus_tag="ACHOQV_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP9629621.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(33313..34317)
                     /gene="cra"
                     /locus_tag="ACHOQV_001224"
     CDS             complement(33313..34317)
                     /gene="cra"
                     /locus_tag="ACHOQV_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFP9629622.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(34399..34485)
                     /locus_tag="ACHOQV_001225"
     CDS             complement(34399..34485)
                     /locus_tag="ACHOQV_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFP9629623.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(34497..34988)
                     /gene="ilvN"
                     /locus_tag="ACHOQV_001226"
     CDS             complement(34497..34988)
                     /gene="ilvN"
                     /locus_tag="ACHOQV_001226"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP9629624.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34991..36715)
                     /gene="ilvI"
                     /locus_tag="ACHOQV_001227"
     CDS             complement(34991..36715)
                     /gene="ilvI"
                     /locus_tag="ACHOQV_001227"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFP9629625.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36834..36878
                     /locus_tag="ACHOQV_001228"
     CDS             36834..36878
                     /locus_tag="ACHOQV_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629626.1"
                     /translation="MKRNDKILHYRGLN"
BASE COUNT         8879 a        10292 c         9006 g         8713 t
ORIGIN      
        1 acgatacaaa tcgatttcaa taaataaccg ctcattctga ttgatggcat tagacagtag
       61 cggtgagctt ttatcaatga gtttgcaaaa ttgtagcccc tgtagattaa cgcctttacc
      121 agtactggta atggcgttgg tgagtgcaaa ggcaaaaatt tcatcttcat ggccaacctg
      181 ccagcaatta ccaaccgacg catacgtccc acagcctgaa gaaatatagc cctgcttctc
      241 accgattatt ctcaggtaaa caatatcgct cactttattt cattccagtt tatccttaac
      301 ttatttgcat taaatagaaa acttaattct cgatcaataa gaaacgcttt taatgaaaga
      361 ttatttcaat tacttaacgt tttcacttag ctcgcaatat tatatttaaa tactcgcgtt
      421 acaaacccat tgtattatta acatctattt attcttatat gaccttttgt aagattattg
      481 tgctggtggc taaggcatag ttttcagcag gcagacaaat ccggcatata gctagttaaa
      541 acagtatgtt atcagactta aagtatctat taaatgtgat gaaaaactta caattgcttt
      601 ttttttatcc aaaaaatcaa cgcatctaca taaagcaggt gcattcccgg gcacatacca
      661 ttatcttgtg atagttaaaa gtagcattat cataattcag ttacatgaaa cgtaaagaaa
      721 tcaatacaac aaacggaatt gcaaacttac acacgcatca ctgcgtagat caaaaaaaca
      781 atcaccgcac gaggtttcat gatggaaggt caacagcacg gcgagcagct aaagcgcggt
      841 cttaaaaacc gccatattca gcttatcgcg ctgggtggcg cgatagggac cgggctattc
      901 ctgggtagcg cctccgtaat acagtccgca gggccaggga ttatcctggg ttacgccatt
      961 gctggtttta tcgcctttct gatcatgcgt cagctaggtg aaatggtggt cgaagaacct
     1021 gtcgcaggct cctttagcca ctttgcttat aaatactggg gcagttttgc cggtttcgcc
     1081 tctggctgga actactgggt actgtacgtt ttagttgcca tggctgagct gactgccgtg
     1141 ggtaaataca ttcagttctg gtatccggaa atcccaacct gggtttctgc cgccgtgttc
     1201 tttgtggtga ttaacgccat caacctgacc aacgttaaag tgtttggcga gatggagttc
     1261 tggtttgcca ttatcaaagt tatcgcggtg gtagcgatga tcatcttcgg cggctggctg
     1321 ctattcagcg gcaacggcgg cccgcaggcg agcgttagca acctgtggga tcagggtggt
     1381 ttcctgccgc acggcttcac cgggctggtg atgatgatgg cgattatcat gttctctttc
     1441 ggcggtctgg aactggtggg gatcaccgca gcagaagctg ataatccgga gcaaagtatc
     1501 ccgaaagcaa ctaaccaggt tatctaccgc atcctgattt tctatattgg ttcgttagcc
     1561 gttctgctct cgctgatgcc atggacccgc gttactgctg ataccagtcc gtttgtactg
     1621 atcttccacg aattgggcga tacctttgtg gctaatgcgc tgaacatcgt ggtactgact
     1681 gctgcgctct ccgtgtacaa cagctgcgta tattgcaaca gccgtatgct gtttggtctg
     1741 gcacaacagg gcaacgcgcc aaaagcgctg gcgtctgtcg acaaacgcgg tgtaccggtt
     1801 aacactattc tggtgtctgc gctggttacg gcattgtgcg tgctgattaa ctaccttgca
     1861 ccggaatccg cattcggact gttaatggca ctggtggtat ctgcactggt aatcaactgg
     1921 gcgatgatta gcctggcgca tatgaaattc cgtcgcgcca agcaggaaca aggcgtggta
     1981 acttgcttcc ctgctctgct ttatccgctg ggtaactgga tctgcctgct gtttatggcg
     2041 gcggtactgg tgattatgct gatgacccca ggaatggcga tttcggtata cctgatcccg
     2101 gtatggctga tcgtgttagg tatcggctat ctgtttaaag agaaaaccgc caaagccgta
     2161 aaagcgcatt aatctctcta cgccctcacc cgtgcagggt gagggcaata atctttacac
     2221 caacgccccg taaatcgtca gtagcgcaat taccaccagg accacgaacg aggttttctt
     2281 cgccatggaa accgctgcct tcggcgtctc caccttatcg acatgcggtt cacgcgccag
     2341 agagaactgt gccagacgtg ttaatacctg atattgcgaa gtatggaaat cacccagcga
     2401 agcaaaccag gccggtaacg ctttctcccc atgaccgatc aaggcatata ccacacccgc
     2461 aagacgaacc ggcacccaat ccagtacatg aagcacggca tcaatgccgg actgtaaacg
     2521 atgatgcggc gtctggtaac gcgccagcca gtattgccat gcacgcaaaa acgcataccc
     2581 catcagcgta acgggtcccc aggttccccc cacaatcagc cagaacagcg gtgcaagata
     2641 aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca cgcaaatact cacgttcatc
     2701 gcagcctgcc gggacgccgt gaatcatggt gagttcgcca gccatcgtgg cacgggcatg
     2761 gctatcatta cgtgaagcag ctgtcagata agcatgataa tgaagacgaa ctttacctgc
     2821 gccaatacac agcaaaccaa tcagcagcca caccagcagc gtgggaacgt tgaacaatac
     2881 tccctgcaat gcgcgtaaca gtaaaaaagt cacgcccatc gcaataatgg tcatgcctaa
     2941 tgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac gcttcaagac gatgatcaat
     3001 ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt aacaccagta aggttgtaaa
     3061 tagcgtcatg ttgtctcctt gctgaccagt gcacgaaacc gtgcccaatc aaatgcaggg
     3121 ccgggatcgg ttttccgatc cggcgcaata tcacaatggc ccgtcatgtt attagcgata
     3181 tctggatagc gatcaatcag tgcccgcgta accgccacaa gctgttgata ctgcgcatcg
     3241 gtatacgcca gcgtatcggt gccttccagc tcaatcccaa tagaaaaatc attgcagcgt
     3301 tcgcgcccct gatactgaga gactccggca tgccaggcac gtttatcgaa aggaacatac
     3361 tggactattt caccatcgcg gcgaatcaaa cagtgagcgg agacgcgcaa atgggcgatc
     3421 tcagcaaaga aaggatgcgc ctgcggatca atagttccag tgaataatgc gtcgatccac
     3481 ggaccgccaa actcgcctgg cggcaggcta atattgtgca ccaccagcag ggtgggtgtt
     3541 tcgtcatccg ggcggcaatc gtaatgtggt gagggaacgc ggcgcgcgcc aaccagccac
     3601 ccctgttcta acaacatgca ggatctcctt atatgtggtg ctaatacccg gttcagagta
     3661 gcatgtttct accttatgat tcgttagcta tctggagttt taacatgccg cctcgccgct
     3721 ataaccctga cacccgacgt gacgagctgc tggaacgcat taatctcgat atccccggcg
     3781 cggtggccca ggcgctgcgg gaagatttag gcggaacagt cgatgccaac aatgacatta
     3841 cggcaaaact tttaccggaa aactctcgct ctcatgccac agtgatcaca cgcgagaatg
     3901 gcgtattttg cggcaaacgc tgggttgaag aggtgtttat tcaactggca ggcgacgatg
     3961 tcaccataat ctggcatgtg gatgacggcg atgtcatcaa cgccaatgaa cccttgttcg
     4021 aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg cactgcactt aattttgtgc
     4081 aaaccctttc aggggttgcc agtaaggtac gccactatgt cgaattactg gagggcacca
     4141 acacgcagtt gttggatacg cgcaaaacct tacccggcct gcgttcagct ctgaaatacg
     4201 cggtactttg cggcggcgga gcgaatcacc gcctggggct ttctgatgcc ttcctgatca
     4261 aagaaaacca tattattgcc tccggctcag tgcgccaggc ggtcgaaaaa gcgtcctggc
     4321 tgcacccaga tgcgccagta gaagtcgaag tagagaatct ggaagaactt gatgaagccc
     4381 tgaaagcagg tgccgatatc atcatgctgg ataacttcga aacagaacag atgcgcgaag
     4441 ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc tggcaacgtc actgacaaaa
     4501 cactgcgcga atttgccgaa acgggcgtgg actttatctc cgtcggtgcg ctaactaaac
     4561 acgtgcaggc actcgacctt tcaatgcgtt ttcgctgatt gctgacagat ttatccgctc
     4621 catcgcgggc ggataatctc ccctttccca actttcttcg taacgcctcg caaatttctt
     4681 gtctttcgct gcaacgtaat aaaaaactcc ggaagctgct ttcccgtttc gttttttgcc
     4741 cctcgcttgt gctgatggcg tttgccaaag tagcgccaac caaatcaagg agcgaaacaa
     4801 atggacaagc aacgcggttt tacacttatc gaactgatgg tggttattgg catcattgcc
     4861 attttaagcg ccattggtat tcccgcttat caaaactacc tgcgcaaagc cgcactcacc
     4921 gacatgctac aaacctttgt gccttaccgt accgccgtag agttgtgcgc gctggaacat
     4981 ggtggattag atacctgcga cggtggcagc aatggcattc cctcgcctac caccacccgc
     5041 tatgtttcag ccatgagtgt ggcaaagggc gtggtgtcgc tgaccgggca agaaagtctc
     5101 aatgggctaa gcgtcgtcat gacaccgggt tgggataacg caaacggcgt caccggctgg
     5161 acgcgcaact gcaatattca aagtgacagc gcattgcagc aagcctgcga agatgtcttt
     5221 cgctttgatg acgccaacta aggagcggca atgaatattc cacagctcac cgccctgtgt
     5281 ctgcgttatc agggagtctt gctggatgcc agcgaagagg tggttcatgt tgcggtggtc
     5341 gatgcccctt cgcatgagct actggacgca ttgcatttcg ctaccaccaa acgtattgag
     5401 atcacctgct ggacgcgcca acaaatggaa ggtcacgcca gtcgcaccca acagacattg
     5461 cccgtagcta ttcatgagaa gcatcagccc aaagcagagt tgctgactcg aacgttacaa
     5521 tctgcgctgg aacaacgcgc gtctgatatt catatcgaac cagcggacaa tgcctaccgc
     5581 atccgcttgc gtatcgacgg tgtattgcat cctttaccgg atgttttacc ggatgccgga
     5641 gtcgcattaa ccgccagatt aaaagtgctg ggcaacctgg atattgcgga acatcgcctg
     5701 ccgcaggacg ggcaattcac tgtcgaactg gcaggaaacg ccatctcatt tcgtattgcg
     5761 accttaccat gtcggggtgg tgaaaaggtg gtattaaggt tgttacagca ggtgagtcag
     5821 gcactggatg tcaacacgct tggaatgcag ccgttacaac tggcggactt tgctcatgcc
     5881 ttgcaacaac cacagggact ggtgctggta acaggcccta caggcagcgg caaaacggtc
     5941 acgctttata gtgccctgca aacgctgaat accgctgaca ttaatatttg tagcgtcgaa
     6001 aatccggttg agatccccat agccggacta aaccagacgc aaatccatcc gcgtgccgga
     6061 ctcacctttc agggcgtttt acgtgcgtta ttgcgccagg atcctgacgt cattatgatc
     6121 ggagagatcc gcgatggcga aacggcagaa attgccatta acgccgcaca aacgggtcat
     6181 ctggtattgt ccaccctaca caccaactcc acctgcgaaa cgctggtacg tttacagcaa
     6241 atgggagtcg cccgctggat gctctcatca gcgcttacgc tggtaatagc ccagcgtctg
     6301 gtacgcaaac tttgcccaca ttgtcgccga cagcaagggg agccaatcca tattccagac
     6361 aatgtatggc cgtcgccgct gccccgctgg caagcacccg gttgtgtaca ttgctaccac
     6421 ggtttttatg gtcgcacggc cttatttgaa gttctgccca taacaccggt catacgtcag
     6481 cttatttccg ctaataccga cgttgaatcg ctggaaacgc acgcccgaca ggcgggtatg
     6541 cgaacgcttt ttgaaaacgg ctgtctggcc gtggagcaag gcttaaccac ctttgaagag
     6601 ttaatccgcg tattggggat gccgcatggc gagtaagcaa ctctggcgct ggcgtggcat
     6661 aaccggcgac ggcaatgcgc aagatgggat gctatgggca gagagccgtg ctttgctgct
     6721 tatggcacta cagcaacaga tggttacccc actaagcctg aagcgaatcg ccatcaattc
     6781 tgcgcagtgg cgaggagata aaagcgcgga agtcattcat caactggcga cgctactcaa
     6841 agccgggtta acgctttctg aagggctggc actgctggcg gaacagcatc ccagtaagca
     6901 atggcaagcg ttgctgcaat cgctggcgca cgatcttgaa cagggcgttg ccttttccaa
     6961 tgccttattt ccctggtcag aggtatttcc gccgctctat caggcgatga tccgcacggg
     7021 tgaactgacc ggtaagctgg atgaatgctg ctttgaactg gcgcgtcagc aaaaagccca
     7081 gcgtcagttg accgacaaag tgaaatcagc gttacgttat cccattatca ttttagcgat
     7141 ggcaatcatg gtggttgtgg caatgctgca ttttgtgctg ccggagtttg ctgctattta
     7201 taagaccttc aacaccccac taccggtact aacgcagggg atcatgacgc tggcagactt
     7261 tagtggcgaa tggggctggc tgctggtgtt gttcggattt ctgctggcga tagccaataa
     7321 gttgctgatg cgccgcccga cctggcttat cgcgaggcag aaattgctgt tacgcatccc
     7381 gattatgggt tcactgatgc ggggacaaaa actcacgcag atctttacga ttctggcgct
     7441 gactcaaagt gcaggcatta cttttttaca gggcgtagag agcgtcagag aaacaatgcg
     7501 ctgcccgtac tgggtgcaac ttctgacaca aatccagcac gatatcagta acggtcaacc
     7561 catctggctg gcgctaaaaa atgccggtga gtttagccca ctctgtttgc aattagtgag
     7621 aacagaagag gcatccggct cgctggatct catgttagac aacctcgccc atcatcatcg
     7681 ggataacaca atggcgctgg cggataacct cgcagcctta ctggaaccgg cgttgctgat
     7741 cataacggga ggaattatcg gtacgctggt ggtggcaatg tatctgccaa ttttccattt
     7801 aggcgatgcg atgagtggaa tgggataacg ctggcgtgtt gctccacgcc agcgttggga
     7861 gattacaggt tgttgaagat gcggttttct tgttccagca cacggataaa tgtggtgcgc
     7921 ttggtcagct ctttcaggcg tgaagcccca acgtatgtac aagctgaacg caggccgccc
     7981 aaaatatcac gcgcggtatt ttcaaccggg cctcgcagcg gcagcttaac ggttttacct
     8041 tctgctgcgc gatattccgc aacgccacca acgtgacgtt tcatcgcaga ctcggagctc
     8101 atgccgtaga acagcataaa tttctcgccg ttctcctcaa cgatgcgacc gccgctctct
     8161 tcgtggcccg ccagcatgcc gccaagcatg acgaaatcgg caccgccgcc aaaggctttc
     8221 gcgacatcgc ccggcgtggt gcagccaccg tcgctgacga tcattccgcc cagaccgtgc
     8281 gcagcatcgg cacattcgat caccgcagaa agttgcggat aaccgacgcc tgttttgacg
     8341 cgagttgtac aaacagaacc tgggccaatg ccaactttaa cgatatcagc accggagagg
     8401 ataagctcct cacacatttc accagtcact acgttgccag cacaaatggt tttggtcggc
     8461 cacgcttcac gcgctttcgc aacgaactgc acgaagtgtt cggaataacc attcgccacg
     8521 tcaatacaaa cgaagtttaa tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa
     8581 tccgcatcag acgtgccggt agaaaccatc acatgtttca gcacatcagc ggaagaattg
     8641 ttgataaacg cttgccactc ttcgaaagaa tagtgtttat gcacagcagt caaaatatca
     8701 aaagaagcca gcgcagaggc catagaaaat gtgcctacgg tgtccatatt tgcggcgata
     8761 atcggcacgc cggaccagct ctgacctgaa tgtttgaagg tgaattgacg ttccagttca
     8821 acatcggaac ggcttttaag agtggagcgt ttagggcgga tgagaacgtc tttaaaacct
     8881 aacttcagat cttcttcaat acgcatgtgc ggattcctgg ggttaatggc tataagggta
     8941 agagcgcaac tccagtgacg ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa
     9001 ttgtcttttt ttacgctaca atcccataaa tttacctggt aaatagtgag cgaacgtttc
     9061 atcaatttcg ctttcgtgcg ctcattttta acgttttgat aatcatgatg aattcccggg
     9121 aatataaatt atgaggtata tagttgcctt aacgggaggc attggcagtg gcaagagtac
     9181 cgttgccaat gcgtttgctg atctcggaat taacgtcatt gatgccgata ttattgcgcg
     9241 tcaggtggtt gaaccaggtg cacctgcgct acatgccatt gctgatcact ttggcgctaa
     9301 catgattgct gctgatggaa cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa
     9361 cccggaagag aaaaactggc ttaacgccct gctgcatccg ctgattcagc aagagacgca
     9421 acaccagatc cagcaagcaa cctcccccta tgtactgtgg gttgtgccat tgctggtaga
     9481 aaactcactg tataaaaaag cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca
     9541 acttaagcgc accatgcagc gcgatgatgt aactcgcgag catgtcgaac aaatccttgc
     9601 tgctcaggca ccgcgcgaag ctcgccttgc cgtggcagat gacgtcattg ataataacgg
     9661 cgcaccggat gctatcgcat cggatgttgc ccgcctgcac gcacactatt tgcagcttgc
     9721 gtcgcagttt gtctcacagg aaaaaccgta atgcagaccc aggtcctttt tgaacatcca
     9781 ctaaatgaaa aaatgcgtac atggctgcgc attgagtttt taattcaaca actcaccgta
     9841 aatttgcccg tcgttgacta cgctggcgct ctgcatttct tccgcaatgt cagtgaattg
     9901 ctggatgttt tcgagcgcgg cgaagtccgc actgagttgt tgaaagaact tgaccggcag
     9961 caacgtaaac tccagacctg gattggcgtg cctggcgtgg accagagccg tattgaagca
    10021 ttaattcagc agttaaaagc ggcggggagc gtattaattt ccgcgccccg tatcgggcaa
    10081 tttctgcgtg aagatcgttt gattgccctg gtgcgtcagc gactgagcat ccccggcggc
    10141 tgttgtagct ttgatttacc tacattgcac atttggctgc atctacccca ggcgcagcgc
    10201 gacagccagg tagaaacctg gattgccagc ctgaacccgc tcacccaggc acttaccatg
    10261 gtgctggatt taattcgcca gtcggccccc ttccgtaaac aaaccagcct gaatggtttt
    10321 tatcaggata acggtggcga tgccgacttg ctgcgcctga atctgtcact cgattcacag
    10381 ctttatccgc aaatttccgg tcataagagc cgttttgcca ttcgttttat gccgctggac
    10441 agtgaaaacg gacaggtacc ggaacgtctg gatttcgaac tggcctgttg ctaaggagta
    10501 aaaatgtcag aaactattac ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt
    10561 gaaatcagcc cgtttcggcc attttgctcc aaacgttgtc agctgatcga cctcggagaa
    10621 tgggctgctg aagaaaaacg aatccccagc agtggcgatc tttccgaaag cgatgactgg
    10681 agcgaagagc caaagcagtg acattttatc tgatgcgacg ctggcctgat aagacgcggg
    10741 cagcgtcgca tcaggcatcg tgtgcaaatg tcggatgcgg cgggaacgcc ttatccgacc
    10801 tacaagacct tacatatcaa catgttaaaa ttgtagatac tgataagacg cgccagcgtc
    10861 gcatcgggca tcttgtgcaa atgttggatg cggcataaac gcctatccga catacacacg
    10921 cttaagcttc gcaagtaccg gttcattggc tggcggaaaa tcatcggcat tcagaccgac
    10981 cagcgacatc cactcaccgg gttgcccttc tttaccccac ggctcccctt cccagctttc
    11041 gaccagccag aaccacagtg ttatatgcct gtccgggaat tcatattcca gtatttcaaa
    11101 tagcgaaaga tgttgtgggg taatcccgac ttcttcctga agttcacgca ccaccgcctg
    11161 ttccggcgtt tcacccattt caattttacc gccgggaaac tccagtttat tcgccatgtg
    11221 cgcatctgct gcgcgacggg ttataaagat ttcattgttc tcgttgcgaa taatacctac
    11281 cgcaatttgc agctttttca ttgtcttaaa cctataaaaa aggcgcagaa tcctgcgcct
    11341 tttacttcaa ctgttagctt ttattgcagg cggccatggc actgcttata ttttttacca
    11401 gaaccacacg ggcaaggatc gttacgtcct actttgcgtt caccggtttg cgccgccagt
    11461 gcagctgcgg cagcagagtc gtcatcctga tggctaagct gctgcatttg cgctaaacgc
    11521 tcggcttcca tacgacgctg ttgttccagc tcctcaacct cttcaggcat acgtacctga
    11581 actttgctca gcgtgctgat aacttcatat tttaacgact ccagcatcgc tgcaaacatg
    11641 gagaacgatt cacgtttgta ttcctgcttc ggatctttct gtgcatagcc acgcaggtgg
    11701 ataccctgac gcagatagtc catcgcagcc aggtgctctt tccacagaga gtcaagcgtt
    11761 tgcagcatga cgcctttctc gaagtgacgc atcatctcag caccaaccac ttcttcttta
    11821 cgctgataca cttcgatgga ctgcgccaga atgcgctcac gcagcgtctc ttcatgcagt
    11881 tctggttctt tatccagcca ctcggcaatt ggcaaatcga ggtcgaaatc gttcttcaga
    11941 cgttcctgca gccccggaat atcccacatt tcttccagcg actgtggcgg aatgtaggca
    12001 tcaatggtcg ctttgaacac atcttcacga atgctgttga tggtttcgct cacatcgctg
    12061 acatccagca gttcgttacg ctgggagtaa atggcgcgac gctgatcgtt agccacgtca
    12121 tcatattcca gcagttgctt acgaatgtcg aagttacggc tttcaacttt acgctgggcg
    12181 ttggcaatcg ctttggtcac ccacgggtgc tcaatggctt cgcctggctt catacccagt
    12241 ttacgcatca tgccggatac tcggtcggaa gcaaaaatac gcatcagcgc atcttccatc
    12301 gacaggtaga aacgggaaga accagcatcc ccctgacgac cagaacgacc gcgcagctgg
    12361 ttatcgatac gacgggattc gtgacgttca gtaccgatga tatgcaggcc acctgctgcc
    12421 agtaccgcat cgtgacgtac ctgccagtcg gctttaattt tttcaatttg ctctgcagtc
    12481 ggattttcca gcgcggcaac ttctgcctgc cagctaccac cgagcacaat atcggtacca
    12541 cgacccgcca tgttggtcgc gatagtcacc gcagccgggt aacctgcctg agcaacaatc
    12601 gccgcttcgt tggcatggaa tttggcgttc aggacgttgt gcttaatacc ggctttggtc
    12661 agttcatttg acaccagctc cgatttttcg atggagattg tacccaccag caccggctgg
    12721 cctttcgcag tgcgttcttt gatatcttca atgatcgcct gaattttttc cgcttcagtc
    12781 atgtagacca ggtccggcag atctttacga atcattggac ggttggtcgg aacaacgaca
    12841 gtatccagct tatagatgga gctgaattcg aaagcttcgg tatcagcagt accagtcatc
    12901 cccgccagtt tttcatacag acggaagtag ttctggaagg tgatcgaagc cagcgtttgg
    12961 ttttcgttct ggatctgcac accttctttc gcttccacag cctggtgcag accatcggac
    13021 cagcgacggc cctgcatggt acgaccggtg tgttcgtcaa cgatgataac ttcaccatct
    13081 ttaacgatgt agtcgacgtc gcgggtaaac agcgcatgag cgcgcagcgc cgccgttacg
    13141 tggtgcatca gcatgatgtt ggccggagag tacagagact caccttcatc catgatgcct
    13201 tctttaacca gcagttcttc aatcagaacc agaccacgtt cggtcaggtt cacctggcga
    13261 gatttttcat ccaccgagaa gtggccttcg ccctggaagg tttcggagtc ttctttttcc
    13321 tgacggatca ggtgtggaat aattttattc acgcgtttat acatttccga gctgtcttct
    13381 gccgggccgg aaatgatcag cggtgtacgc gcttcatcga tcaggatgga gtccacttcg
    13441 tccaccagcg catagtgcag tttacgctgt acacgttctt caggactgaa cgccatgttg
    13501 tcgcgcaggt agtcaaagcc gtattcgttg ttcgtaccgt aagtaatgtc agctgcgtaa
    13561 gcttcacgct ttgccggtgc tggcatgccc ggcaggttga taccaactgt caggccaaga
    13621 aattcaaaca gcggacggtt gttttcggcg tcacgttgcg ccaggtagtc gttgacggta
    13681 actacgtgca cgcctttacc ggttagtgcg ttcaggtaag caggcagcgt tgcggtcagg
    13741 gttttacctt caccggtacg catttcggcg atgcagcgtt cgttaagaac cataccgccg
    13801 agtaactgaa cgtcgaagtg acgcatacca aagacgcgct tacttgcctc acgcaccacg
    13861 gcgaaagctt ccgggatcag attttccagc acttcgcctt tttccagacg tgcacgaaac
    13921 tctgcggttt tccctttcag ttcttcgtcg gagagttttt ccatctccgg ttccatggca
    13981 ttgatgatgt tgaccacttt gcgcatccgg cgcagggtgc gatcgttacg actaccgaaa
    14041 actttagtta ataatttgat tagcataata aaatctcaaa cgccccgcgt tgcggagtta
    14101 ataaaatgaa gtaacggttt attgttgtta gctgaggcgt tgagggccag cacggatgcc
    14161 ttgcgcctgg cttatccaga cgggcgtgct gaattttgct tgcggggtaa aatgcgcata
    14221 atcaatgcga taaccctttt cagacggcgt gccttcctgg gtcaacagcg cgctgagcgt
    14281 atccagtaat gcaagatgtt gcgcctgaag aggcaaagat tcttcagcaa cgggcagcgt
    14341 ttgcggtgcc attgcgaagg aaagatgacg gattaccgtg cgaatggcat gttgatgcca
    14401 gtaatcaacg gaatagttcg aattcgggcg acgcgtgttc gcttccagca aggccaattg
    14461 accaaagtta actttggctg aaggctcgtg gttgcgggtt gtcgcttttg cgggcgcgtt
    14521 tggttcggcg gcgttgctga gcgcaggcaa acctaaactc gccgcgacca tccctaataa
    14581 gagatgcggc cagaagtagc gtttaccaaa ctgtcgccag cgcgtcagta ttccactcac
    14641 gttattgcca tcccgatcgt attcaaacgt atttagcgca caaatcttat cattaaagcc
    14701 tgaagtccac agcacgaatg aagataagtt gtgcgtaaaa gtgcttaaga aaacagcgtt
    14761 cgcaccgttt tttatcgttc ttataaaatt caaaaaaaaa cgactggttc acctggccgg
    14821 agagagtgcc agatttgcca gtcgaatttt atacgacagt ataaatgtcg ttatgccagt
    14881 acggctgaag gcgctttgaa ggccaacggc agttctgcgt cgtcctggaa ggtcacatat
    14941 tcccaggctt cctgtttcgc caggacagcc tgcagcagtt tgttattcag tgcatgacca
    15001 gatttataag cggtaaatgc accaataata ttgtgaccac acatgaacaa gtcaccgatc
    15061 gcatcgagca ttttgtgacg cacaaattcg tcttcaaaac gcaggccgtc ttcgttcagt
    15121 acgcgataat cgtcaacaac gatggcacaa tcgaagctgc cgcccaggca caaaccacgg
    15181 gactgcagat attcgatatc acgcatgaaa ccgaacgtac gcgcacggct gatctggcgc
    15241 ataaacgcat cagcggagaa gttcatcgca tagcgctggt tgctggaatc aatagccgga
    15301 tggttaaaat cgatggtgaa atccagcgaa aaaccattgt acggcttaaa ttcagcccac
    15361 ttatcgccat cttcgacacg aacagtctct ttgatgcgaa caaatttctt ggcgcagttc
    15421 agctcgtcga taccagcgtc aagcagcagg tatacaaacg gagcggcgct gccgtccatg
    15481 atcgggattt ccggcgcgtt aacttcgata acaatgttat cgatgcccag gcccgcgaga
    15541 gcagcattga ggtgctctac ggttgaaatc cgtacatcat gctcgttgac cagacacgta
    15601 cagagcatgg tatcacgcac agatttggca tcggccggga aatctaccgg tggattcaag
    15661 tcggtgcgac gatagatgac cccggtgttg gccggcgcag ggcgtaacgt cagggtgact
    15721 ttcttgccgg tatgtaaacc gacacccgtc gcctgaacga tacgtttaag tgtcctttgt
    15781 ttgatcatcg tattatctcg ccaaattacc tatccaaccg aagtgtacta tacattcggc
    15841 gggccagttt agcacaaaga gcctcgaaac ccaaattcca gtcaattctt aatcagcttg
    15901 cttacgcagg aatgctggga tatccagata atccggctct ttcgcagttt gcggcgcatt
    15961 gtcattcacg actttagcaa ccggcttctg ctcctgggtc agcggagcca tcccgtgctg
    16021 ctggtagcga tccatcactg gctgctgaac ctgcttattg gtcaccagag tgatttcagg
    16081 acgtttgtcc atgccgatac ctgtcgcaac aacagttacg cgcagctcgt cattcatatc
    16141 cgggtcaaga gaagtaccga taaccacagt cgcgttgtcg gaagcaaatg cacggatggt
    16201 gttacctacc gtttcgaact catccagacg caggtcgaag cccgctgtga tgtttaccag
    16261 tacgccgcgt gcgccagaca ggtcgatatc ttccagcagc ggagaagaga tagccatttc
    16321 agcagcttct tccgcacgat cttcaccgct cgccacacca gaacccatca ttgcgtagcc
    16381 catttcagac attacggtgc gtacgtctgc aaagtccacg ttcatcaaac ccggacgagt
    16441 aatcagttcg gcgatacctt gcacagcgcc tttgagtacg tcgttcgctg cgccaaacgc
    16501 atccagcagg gagataccgc ggcccagaac tttcagcagt ttgtcgttcg ggatagtgat
    16561 cagagagtcc acatgcttgg acagttcagt gatcccctgc tccgcgaatg ccatacgctt
    16621 cttgccttca aagttgaagg gcttagtgac gacagcaacg gtcaggatac ccaaatcttt
    16681 tgccacttca gcgacgactg gagctgcgcc tgtaccggta ccaccaccca tacccgcagc
    16741 aataaagacc atgtctgcac cttccagcgc cgcacgcagt gcatcgcgat cctcatcagc
    16801 cgcattgcgg ccaacttctg gattagcgcc agctcccagt cctttggtga taccgctacc
    16861 gatttgaatc gtctgtccaa ccgctgtttt acgcagcgct tgtgcatcgg tatttaccgc
    16921 gaagaattca acaccttcaa tgcgctcgcg caccatgtgt tcaacagcat taccgccgcc
    16981 gccgccgacg ccgatgactt taatcaccgc gtcattggta agttccattg gttcaaacat
    17041 agtttctctc cgatttgtgc ctgtcgcctg agaccgtaat cattgtcggc ctcataaaaa
    17101 ttaaaactct tttcgcagcc aactattgag tcgcttgatc cacgagccaa ctgatgctgt
    17161 aacacgtttt tctacttcag cttcaccgtt aagatgtgac tctttcccgt aatgaagcaa
    17221 tcccaccgcc gtcgaataat acggctcctg agcataatcc gttaaaccgg taatgttcag
    17281 cggcgcgccg atacgcacct gcgtatgaaa cacgcgctga gcacaggctg caagaccttc
    17341 aatctgcgct gcgccaccgg ttaatacaat gcctgccgcc aggtgatgtt taaccccttg
    17401 ttggcgaagc ttttcctgca actgcaatat ctcttcgttg accaggttga gcagctcggt
    17461 atagcgcggc tcgatcacct ctgccagggt ctgacgttgc agactccgcg gcggacgacc
    17521 gcctacgctc ggcacttcca cgctctcatc ttttccaacg atggaaccca gcgcacaacc
    17581 gtggcgaact ttaatcgctt cggcgtcgct tggcggcgtg ccaaaggcgt aagcgatatc
    17641 actggtcacg acattgccag cataaggaat taccttagtg tggcgcaatg ccccaccggt
    17701 ataaacggcg atatccattg taccaccacc gatatcgacg acgcagacac ccagttcacg
    17761 ttcatcttcc gtcaataccg aataacttga tgccagtccg gcaaatatca gttggtcaac
    17821 tttcagccca caacgttcaa ccgctttgac gatgtttttc gccatatcgt tgtgacatgt
    17881 gatcaggtgc acttttgcct gcatccgcac gcccgaaagt cctaccggat tcttgatccc
    17941 ttcctgatag tcaatcgcat actcttgcgg gatcacatgc agcacacggt gttcatcgcg
    18001 cacacgcacc gatttcgcgg tatggacaac gttttccaca tcttcttgcg tcacttcttc
    18061 ttcagaaata ggcaccatac caatttcatt ctggcagctg atgtgcttac cagaaagcgc
    18121 cagatatacc gaagagatct gacaatctgc catcaattct gcctggtcaa tggcgcgctg
    18181 tacgcacttg accacggatt cgaggtcgtt caccccgcct ttatccatac cacgcgacgg
    18241 gcagctgccc acgccaatga tattgaccat accgtcgggc agaacttccc ctactaaagc
    18301 ggcaaccttc gcggtaccaa tctccagtcc tactaccagt tttctgtccg tcgccttaat
    18361 cattgttgtt ctgcctgtgc ctgattttgt tgctgagtag attcctctgg cggcaagggc
    18421 gcccagccta ctgccgctcc agagtcataa cgcaaatcaa cgtagctaat ccgtttgcca
    18481 tcggtttgcg cctgctgctg taaaaccgga taaagttcta caaagcgagc caaacgtttc
    18541 atcgtatcgc cccggccaag attgagctta atatcgttat tcagcgtcaa ctgccaggaa
    18601 cgccgcgcgg tcatcgccgc ttccttcaga gtaaatctgt cctttgccag catctgcccc
    18661 atttcgcgat agccctgcaa cacttcattg gcgctgcctt ccgggccata cagcattgga
    18721 agcacctgct tgctggtgcg atctggcggc acgctgaaag tgtttccttc cgcgtctacc
    18781 atatgttgat cattccaccg cgcaatcggc acatattcaa ccagatgaat cttcaattca
    18841 tcaggccact gctttctgac gctcacctgc ttaatccacg gcaggcgttg ttctatttgc
    18901 gtctggatga tgttgacatc ctgggtcata aaggtacctg gctcacccaa tgccaggatc
    18961 gactgccgga tatcgtcatt acgcgtgtaa tggcgttcac cggtcaacac cagctttgag
    19021 agcggcaggc gttgcgcatc ttccatccag cccaacacga cccagccgct caccaacact
    19081 gtcgttaaaa cggtcagcag gaaaaggatc cccgccagac gcgttccatt attgcggcga
    19141 gaagaaacct cttcttcgct gtttcgcgtg ttcagagcag cctgcgacat attagtccgc
    19201 cagttccaga attcgtacta ccaactgcga gaagctcata cctgcctgac gtgccgccat
    19261 cggcaccagg ctgtggctgg tcatacccgg tgaggtattg gcttccagca gataaaactg
    19321 tccatcgctg tccagcataa cgtcaatacg tccccatcct ttgcaaccta acgtcgtcca
    19381 tgctttcagc actaatgcct gcaaattggc ctcttgtgac gcttccagac ctgcggggca
    19441 gaaatactgt gtctcatcag agagatactt cgcctcataa tcatagaagg ttccggacgg
    19501 ttgaatacgt attgacggta aaatttcttc accgagtatc gcaaccgtga actccggccc
    19561 acttagccat ttttcaatca atacttcttc atcgtgctga aatgccaatc ttaatgcatc
    19621 ttgtagagca ttttctgcta ctacttttga cattcccaca ctggaacctt cgcggctcgg
    19681 cttaacgata accggcaaac ccagagcaga aatttctgct aactgcttat cactcaggcc
    19741 tttttcaaac tctgcgcggg ttaacgctac ccacggagcg accggtaaac cggcaccttg
    19801 ccacagaagt ttgctgcgta gtttatccat tgaaagcgca gatgccatca ctccgctgcc
    19861 ggtataaggc aagcccatca gctcgagcat gccctgcagc gtgccatctt caccgccgcg
    19921 accgtgtagc gcgataaaca ctttctgaaa gcccatcgac ttcagttgcg tcacgtcgac
    19981 ttctttcggg tcgacaggat tcgcgtcaat accgccttca cgcagcccgg ctaacaccgc
    20041 tgcgccagaa ttcagagaaa cttcccgctc agcggaggtc ccacccaaca ggaccgcgat
    20101 tttatcagtc atgttgttct tcctccggag tttgcggctt cagtttgatt tcagctaaag
    20161 aacgggcaat ttttccgata ttaccagccc cctgaacgag aatcaggtcg ttaccggtta
    20221 ataccggagc cagcatctcg gctacctgcg ccgggtccgg caccagaatg ggatcaattt
    20281 tcccacgtcc acgaattgtg cgacacagcg aacggctgtc cgctcccgga attggcgctt
    20341 cgccagccgg atacacttcc agcatcaaca gggtatcaac ctgcgtcagc acattggcga
    20401 aatcatcata caggtcgcgc gtacgggtaa aacggtgcgg ctgaaacagc attaccaggt
    20461 ttttatccgg ccagcctgca cgcgccgctt taatggtggc gtccacttcc gtcgggtggt
    20521 ggccgtagtc atcgaccagc attgccgtac cgcttttacc attcacaggc tccagcggga
    20581 attcaccgag gaaatcaaaa cggcgaccag tcccctggaa gctctccagc gcacgcaaaa
    20641 tagcctcgtc gtcaattccc tcttccgtag caaccgcaac cgcagctgcg gcgttcagcg
    20701 cgttatgacg gcctggcgca ttcagggtga cgcgcatcgg ctctttgtcc tggcgcagca
    20761 gcgtaaagtg cccctgcggg ccaatctgct gataatcttc tacacgcacg tcggcatctt
    20821 cgctgaagcc gtaagtcgtg gtctgacgtc ccacacgcgg taataattcg cggatcaccg
    20881 gatcatcaac acacatcacc gcacgaccgt aaaacggcag gttgtgcaga aaattaataa
    20941 aggtctgttt taaattctca aagtcgccct ggtaggtatc catgtggtcg gcttcgatat
    21001 tggtgacaat cgccaccatc ggttgcagat gcaggaacga tgcatcactc tcatctgctt
    21061 cggcaatcag gtaccgacca tgccccaaac gcgcatgaac gcccgccgct tttaccagcc
    21121 caccgttaac gaaggttggg tcgagtcccg cttctgcgta gatgctggaa accatcgcag
    21181 tggttgtcgt tttgccgtgc gttccggcaa tggcgatgcc atgacgaaaa cgcattaact
    21241 cagccagcat ttcggcacga cggatcaccg gaatacgcgc ttcatgagct gcgacaattt
    21301 ccgggttatc ggcagaaatc gcgctggaaa caacgaccac gctggcatca cgtacgtttt
    21361 ccgggcgatg gttgaaataa atcgtcgccc caagattcat taactgctgc gtgaccggat
    21421 ttggcgctaa atcggaacca ctgatctgat aaccttcatt ggccagaact tcggcaatac
    21481 cgcccatacc ggcaccaccg atgccgacaa aatgtatgtg ccgaacgcga cgcatttcgg
    21541 gcacgatgga acgcagtttt gccaattgtt gtgtattcat tctttacgcc attaacttct
    21601 taaattcata cgatgcaaaa ggcatcgcta caattacgcc cgggcagccc ggctcacttc
    21661 atttgccact cgctcggtgg catccggaat ggatgcggcg cgggcgcgtt ctgccatggt
    21721 taataaggtt tctcgcgacc acccggccag ggtgttggcg acagcatcca cgctaagctg
    21781 tggctgctcg ataattttgg ctgcgcccgc tttttccagc ggtagcgcat tccagtattg
    21841 ctggcggtct ttatgttgaa acggcacaaa caacgccggt agtcctgccg cggcgatttc
    21901 actcaccgtt aacgcaccgg agcggcaaac gacgacatcc gcccacgcat acgccgccgc
    21961 catatcatca ataaattccg tcactttatg ctgcggttgc cccgcttcgg catacgcctg
    22021 ttcaacggat tgttgcgaac ctttaccgct ctgatgccag atagtgactg aatcacccag
    22081 tttcgcagca acctgcggca ttgtctggtt aagaatgcgt gcgccctggg aaccacccac
    22141 taccaacaca cgaaccggac cttcacgtcc agccaaacgt tgctgcggca acggcagcgc
    22201 cagcacatcg gtacgcaccg ggttacccac cacttccgca ttagggaaag cgcctggaaa
    22261 cgcctgcatc actttggtgg caatcttcgc cagccattta ttggttaatc ccgcaatacc
    22321 gttttgttca tgaagcacaa ccggaatacc taacgaccac gcggccagac cacctggacc
    22381 tgatacgtag ccgcccatac cgagcaccac gtcaggtttg tacgctttca taatcgcccg
    22441 cgcctgacgc caggcgttga agatacgcag cggggcagct atcagtgctt ttataccttt
    22501 tccacgcaga ccagagatac gaatgaaatc aatttcgatg ccatgttttg gcactaagtc
    22561 cgcttccata cggtcggcag tccccagcca gcgaacttgc caaccctgag ccattagatg
    22621 gtgcgcaacc gccagtcccg ggaatacatg tccaccggtt ccgcctgcca tcaccattaa
    22681 tcgcttccct tgaccactca tcgtgaacct cgtacaaacg cctgcgcttt ctccagacgc
    22741 gtttcataat caatacgcaa cagcatcatg atggctgtcg acataatcag taagctcgaa
    22801 ccaccgtaac tgatcagcgg caatgtcaga cctttggtcg gtaacatccc cgccgccgcg
    22861 cctacgttaa ccagcgcctg gaaactaaac cagataccaa tagaacaggc gagaaaaccg
    22921 gaaaaacggt ggtcaatttc taatgctttg cggccaatcg acatcgcgcg aaaagcgacg
    22981 aagaatacca ttaaaagtgc cagcaccaca ccgacatacc ccagttcttc gccgataatg
    23041 gcgaaaataa agtcagtgtg cgcttccggc agatactcca gtttttgtac cgagttacct
    23101 aaaccttgtc cccacagttc accgcgacca aacgccatca acgattgcgt taactgatag
    23161 ccgctgccaa agggatcttc ccacggattc cagaatgcgg taacacggcg gatacggtac
    23221 ggttcggcga ggatcagcaa cacaaccgct gaaatgccca taccgataat ggcaatgaac
    23281 tgccacaatt tcgctcccgc caggaacaac atcgccagcg tagtcacaaa caacaccacc
    23341 accgtaccaa ggtctggctg tgccagcagt aacactgcca acaccagaat tacgcccatc
    23401 ggtttcagga agccgcgcag gttattacgt acttcgtcgc ctttacgcac cagatagttg
    23461 gcgatatagc aaaacagcga cagttttgtc agctccgcag gctggatacg cagcaaaccg
    23521 agatcgatcc aacgcgatgc ccctttaacc gagctaccca ctaccagaac gatcatcagc
    23581 aggatgatag atccgagcag catcgtggca ctgtagcgtt gccagaactc catcggcaga
    23641 cgcagcgtaa tgatcgccag aatgaatgcc aggatcagat agacgccatc acgcttcgcg
    23701 aagaagaacg gatcgttggt taagcgttgc cctatgggca ttgacgccga ggtcaccatg
    23761 ataaagccaa tcgccgcgag gccgaaggtc agccacagta aggtgcgatc gtacatgatc
    23821 aggctgtcgg tatctttttc ccgcgagccc atcacccagc cctttagcgc cgtggagatc
    23881 cagaccagaa tactgaatcc tggcaggcgc ggcattttca ggcgagggag agataaacgc
    23941 atcaacctaa ctccttcgcc agacgggcaa actcattgcc tcgttgttca aagttcttga
    24001 attgatcaag gctggcacag gccggggaga gcagaaccat atcgcccggc tgaacacgcg
    24061 gagccagcaa gcgcatcgcc tgttccatgg tttcggtttg ttctgccact tccgggcgta
    24121 gcgccgccag ctgcgcgccg tcccgaccga aacaatacag acgtacatta tcgccattca
    24181 ggtaacgcgc cagtgggcta aagtccgccg atttaccatc gccacccagc aacaaatgca
    24241 gcgtgccgtc tacatgcagg ccattcagcg ccgcttccgt actgccgacg ttggtcgctt
    24301 ttgaatcgtt aacccagcgc acaccgttat gctccagcac aacttcaaag cgatgcggca
    24361 gaccagtgaa tgtggttaac gctttcaggc tgctggcacg cggtaaccct gcggcatctg
    24421 ccagcgccag cgccgccagc gcattggtgt agttatgctg cccggaaagt ttcatttctt
    24481 tcacgttcag cactttctcg cctttcaccc gcagccaggt ttcaccctgc tgatgattca
    24541 ggtgatagtc gcccatgttg acgccaaagc tgacgcagcg ttcatccgca ccgcgaatcg
    24601 gcattgttaa ggcatcatca gcattaacca cgcaaatttt tgcgttttcg taaatgcgca
    24661 gttttgctgc acgatactgt tgtaaaccaa acggatagcg atccatatga tcttcagtca
    24721 cgttcagaat ggtcgctgct accgcctgta aactggaggt ggtttcaagc tggaagctcg
    24781 acagctccag cacgtacagt tcgcactcat catccagcag catcaacgca ggcaggccaa
    24841 tattgccacc cacaccaacg ttaacccccg ccgctttcgc catttcaccc actagcgtgg
    24901 tgaccgtgct tttgccgtta gaaccggtga ccgccacaat cggtgcttgt gcttcacgac
    24961 agaacagctc gatatcgcca acgatttcga ttccggcatc agcggcagcg cttaaggatg
    25021 gatgcgccag tgcaataccg ggactggcga caatcagatc ggccgccatc agccattcat
    25081 cattcagacc gcccgtgtgg cgttctacag cttcgggtaa tttatccagg ccaggcggtg
    25141 tcatacgcgt atccataacg cgcggcgtca caccgcgagc gaggaaaaag tccacgcaag
    25201 aaagcccggt gaggcccagg ccgataatga cgacattttt accctggtaa tcagccatga
    25261 ttaacgtacc ttcagcgttg ccagaccaat caggaccagc atcagcgaaa taatccagaa
    25321 acgtacaatg acgcgcggtt ccggccagcc tttcagttca tagtggtgat gaatcggtgc
    25381 catgcggaaa atacgttgtc cgcgcagttt aaaggagccg acctgcagga tgacggaaag
    25441 tgtttctacc acgaacacgc cccccataat caccagcagg aattcctgac gcagcagtac
    25501 ggcgataata cctaacgcac cacctaacgc cagcgaaccg acatcgccca taaagacctg
    25561 cgccggatag gtgttaaacc acaggaagcc cagccctgcc ccgactatcg cggtacagac
    25621 aatgaccagt tccccggcgt gtcgtagata cggtatgtgt aaatagctgg caaagttcat
    25681 gttgccggtc gcccacgcca ccagcgcaaa accaccggcg acaaatacgg tcggcataat
    25741 tgccagaccg tcgagaccat cagtcaggtt taccgcgttg cctgtaccca caatgacgaa
    25801 gtaagccagc agaatgtaga acagccccag ttgcggcatc acatctttaa aaaatgggac
    25861 caccagctgc gttgcgggcg tgtctttgcc aacaaggtac agcgcgaagg cgacacccag
    25921 cgcaatgacc gacatccaga aatacttcca acgagcgatc aaccctttgg tgtctttacg
    25981 cactactttg cgataatcat caacaaagcc aataacaccg taacctacca gcactaccaa
    26041 cacgcaccag acgtacggat tggacgggta agcccacagc agtacggaga tcacaatcgc
    26101 tgtcaggatc ataatcccgc ccatggtcgg cgtaccgcgc ttgctgaagt gtgattcagg
    26161 accgtcgtta cgcaccacct gaccaaagga aagtttttgc aaatgagcaa tcatgcgcgg
    26221 gcccatccac aatgagatga acagcgcagt cagcaggctg acgatggcgc gaaacgtcag
    26281 ataggaaaag acgttaaagc cggaataata tttgaccaaa tgttcggcca gccaaactaa
    26341 catgtcccat tctcctgtaa agcgcgtact acctcttcca tggcagcact acgtgaaccc
    26401 ttaactaaaa tcgtaattac ctgttgctca gcaatcagtg atttaagacg cgcgataagc
    26461 gcagttttat cggaaaaatg ttcgccaaca ccgctggcag tgctgatagc atggctttgt
    26521 ttgcccacgc ttaacacgcg gtcaatacca gctgcttttg ccgcttcgcc cacctgtata
    26581 tggcaggctt cgctttcagc gcccagttcc gccatatcgc ccaccaccag cacgcggtag
    26641 cccggcattt cagccagtac ctggactgct gcggtcattg aaccgacatt ggcgttgtag
    26701 gagtcgtcga gcagcaactg gttttccgcc agttggatgg ggaacagacg gcctggaaca
    26761 gctttcagat ttgccagccc cgctttgata gcatcaagcg ttgcgcccac ggacatggag
    26821 agcgcagcgg ctgccagcgc attcgcaata ttgtgacgcc ccggcaacgg cagcagaaca
    26881 tccacgctac cggttggggt ttgcagggta aattccgtac cgtgcgaagt cacatggatg
    26941 ttggtggcgg tgaaatcgct gttggcggca ttaggtgaga aacgccacac tttgcgtgag
    27001 ccaattacgc tctgccagtt cagccagtcg ttgttgtcgg cgttcataat ggcgataccg
    27061 ttttccggca ggccgctaaa gatttcacct ttcgctttcg cgacacccgc aagcgagcca
    27121 aaaccttcca gatgcgccgc tgccaggttg ttgaccagcg ccgcttccgg gcgagtcaga
    27181 ctcacagtcc aggcaatttc gccctgatgg ttcgcgccaa gttcaattac tgcgtaatcg
    27241 tactctggcg ttaagcgcaa cagcgtcatc ggtacaccga tgtcgttgtt gagattgcct
    27301 gccgtataaa gcgtgttgcc acactggctt aaaatcgctg ccgtcatctc tttaacggag
    27361 gttttgccgg aggaaccggt cagagcaacc acgcgcgccg gaacttgctg gcgaacccat
    27421 gcagccagtt caccaaacgc cagacgagta tccttgacga ttacctgcgg cagatcgata
    27481 tccagcggac ggttaaccag tagtgcaccc gcaccgccag ctttcgcctg gtcagcaaaa
    27541 tcatgggcat cgaaacgttc gcctttcagg gcaacaaaca ggcaacccgg cgtcagtttt
    27601 cgtgtgtctg tggttacagc atcaagggtg atatctgccc cttttagttc accgttgaga
    27661 atatcggtaa gttggctaag ggttacgcta atcatgcaat cacccccagc agacgcgcca
    27721 ccgtgacgcg atcggagtag tccagacgct gattgccaac aatctggtaa tcctcatgac
    27781 ctttgcccgc gaccagtacc acatcattct ctttagcctg cataacggcg caagtcaccg
    27841 cttcagcacg gccttccatc actttggcat gtccggcatc taacattccc gccagaatat
    27901 cgttgatgat ggcacgcggt tcttcggtac gcgggttatc gtccgtcacc accgccacgt
    27961 cagcgaactc ttcagcaatt gcgcccatca gtggacgttt gcctttatca cgatcgccac
    28021 cacagccaaa gacgcaccac agcttgcccg cacagtgcag gcgcgccgcc tgtaaggctt
    28081 tttccagtgc atccggtgta tgcgcgtaat ccaccactac cgtcggtttg cctggcgcag
    28141 tgaacacttc catacgtcca caaaccggct gcaggcgtgc ggcagttttc agcaggtcag
    28201 ccaacggata gcccagcgcc agcagcgtcg ccagcgccag cagcaagttg ctgacgttaa
    28261 aagcgcccat cagatggctt tcaatttcgc catcgcccca gcttgagcta aagcgaatcg
    28321 tcgcaccgct gtcgtgatag ttcacttctg tcgctttcaa ccagcgtccg tgacagttcg
    28381 gattgatatg atcttccatt gataccgcaa ccgcgtccgg cagttttgcc agccagcggc
    28441 ggcccacttc atcgtcggcg ttaataatcg cctgaccgca atgatgctca gaataaagca
    28501 gccatttcgc ggcttcgtag tgttccatat caccatgata atcaaggtga tcgcggctta
    28561 agttggtaaa gaccgacgcc gcaaatttca atgccgccac acggtgctgt accagtccgt
    28621 gggaggaaac ttccattgcg caaaaagtcg cgccctgatc caccagcccc gccagctcat
    28681 gctgaacatc gactgccgaa ccggttgtat tttctgtcgg gatcactttc cccagcaggc
    28741 cgttaccaac ggtgcccatt accgcgctgg tttcgccaag cagttggctc cactgcgcca
    28801 gcagctgggt cgtcgtggtt ttgccgttgg tgcccgttac gcccacgaga cgtaaattgt
    28861 cagagggttc atggtaaaag cggcccgcca gtgcagataa acgctcgttg agctggctga
    28921 gatagatgac cggtacgccg tgcatttcac ggatttcacc gtcggtcgcc tcatctttcg
    28981 cctctgcaat aatggcagcc acaccttgcg ctatcgcctg cgggatatat cgacgcccgt
    29041 ccgcctgatg acctactaca gctacaaaga gatcgcccgc cgcagccaca cggctgtcga
    29101 gtgtcatctc tcgcagtgct cgcgaaggtg cgtctggcac ccacggagca agaaggtcgc
    29161 gcaaattacg atctgccacc tgtcccctcg ccttgattaa tcacaaattc atttttatcg
    29221 cccgttgtca gcgcatccgg ttcgatgttc atggtacgca atacgccgcc catgatggca
    29281 ccaaagaccg gcgcggaaac ggcgccgccg tagtatttac ccgcctgcgg atcgttgata
    29341 acaacaacca gcgcgaagcg cggctgactc gcaggcgcaa cgcctgcggt ataagcaata
    29401 tatttattga tgtagcgacc gtccggcccg acctttttcg cggtaccggt tttaatggcg
    29461 atacgatagc ctttaatcgc cgccttcacg ccgccgccgc ccggtagcgc cacgctttcc
    29521 atcatatgca ccacggtgcg aacaatggat tccgggaaga ctcgttcacc gggaaccggg
    29581 gggtcaactt tggtaatcga cagtgggcga taaatgccgt agctgccgat agttgcgtag
    29641 actcgcgcta actgtaacgg tgttaccatt agcccgtagc cgaaagagaa ggtggccctc
    29701 tctatgtcag accaccgttg tttttgagga tataagccac tgcgttctcc gaccaacccc
    29761 aaattggtcg cttttcccag tccaaaacgt gagtaagtat ctactaacgc tgaggacggc
    29821 atcgctaacg ccagcttgga aacaccgacg ttactcgact tctgtaatac cccggtcagg
    29881 gttaattcgc tgtagcgtgc cacgtctttg atttcgtggc cgttaattcg ataaggaacg
    29941 gtattcagta ccgagttttc ccgcactacg ccacgttgca acgcggtcat caccaccatc
    30001 ggtttaaccg ttgaacccgg ttcaaacacg tcagtgatgg tacggttacg catcgcctct
    30061 ttcggcgtgc cactcagatt gtttgggttg tatgacgggc tgttagccat cgccagcact
    30121 tcaccggtgt tgacatccac cagcacggcg ctaccagatt cagctttgtt aaaggccacc
    30181 gcgttgttca gttcgcgata aaccagcgcc tgcaggcgtt catcaatact cagcgccagg
    30241 ttgtgcgccg cctgactgtc ggtggaagag atatcttcaa ttacgcgacc atagcggtct
    30301 ttacgcacaa tgcgctcacc cggctgcccg gtaagccatt tatcgaaact cttctcgacg
    30361 ccctcaatcc cctgactatc gacgttagta aagccgatga ggtgagcggt cacttcgccg
    30421 gatggataat aacggcgaga ttcttcacgc agatgaatcc ccggcagttt cagttttttg
    30481 atgtactctg ccatgtcagg gtttacctga cgcgccagat aaataaagcg ccctttcggg
    30541 ttggcgttaa tgcgggctga aagctgatcc agcggaatat tgagtgcgtt agccagcgcc
    30601 ttccagcgat caccgacgct gataccgcca gcgtcatgca cttctttcgg gtcagcccaa
    30661 atcgctttta ccggcacgct caccgctaac gggcgaccag aacggtcagt aatcatgccg
    30721 cgggaggtgg aaacttgctg aacgcgaaga gaacgcatgt cgccctcttt taccagcata
    30781 tccggggaga taacttgtaa ccacgctacg cgtccgagca gaaaagccag cgccaggaga
    30841 atacagccgc ataacaacgc aaaacgccaa ctgataaagt tggcatgttc ttcctgacgt
    30901 tttggtttct gcgttttcgc cgctgctttc atgcgtcgcg tttatcctta tttttgcact
    30961 acgatatttt cttgtgacgg atcaacatgc tgcatttgca gcttttccgt ggcgatcctt
    31021 tccacccggc tatggtcgcc gagcgcattc tcttcaagga tcaggttgcg ccattcaatg
    31081 tctaaagcat ctcgctccag caccagttgt tcgcgctgtg cggtcagtaa acgggtatgg
    31141 tgcgccgtgg ttaccacggt caccgccgtc aaaataatgc aaatgaacag gcagagtggc
    31201 agcttcccaa atcgcaaaag atcgtcaccg ataacaccag gcaatgcatg gcgctcgtgg
    31261 cttcccatcg atcctttaac tttgcttaga gcttctgtca ctctgctgat catgcgttcg
    31321 tcctctctgc aatacgcaga actgaactac gggcacgagg gttctcagcc acctcttctt
    31381 cacccggcat taacttgcct agtgctcgca gctgacggcc acccagtttt ttgagctgct
    31441 cttcagtcat cggtaaccct gccggaactt gcggaccgcg gctgttttca cgcataaaac
    31501 gtttcacaat acggtcttcc agcgagtgga agctgatgat cgaaagccgc ccacccgggg
    31561 ccagcacgtt gagcgagctt tttagcgcct gctctatctc ctccagttca ctgtttaccc
    31621 aaatgcgcac cgcctggaag gtacgggtcg cgggatgttt aaacttatct ttcaccggcg
    31681 ttgcagcagc cacgacttcc gccagttctt tggtgcgggt catcggctgt tcgcggttac
    31741 gctcgacaat ggcgcgggca atgcgtttgg caaaacgctc ttcaccatag gttttcaata
    31801 cccaggcgat atcggcttct tctgcggttt gtagccattc agcggctgac tgcccacggg
    31861 ttgggtccat acgcatgtcc agcggaccat cgcgcataaa ggaaaagccg cgttcagcat
    31921 catcaagttg cggtgaagag acgccaagat cgaggagaat gccgtcgatc ttgccgataa
    31981 gatcgcgctc ggcaacgtat tcgcccagcg cggagaaagg tccgtggatg atggagaagc
    32041 gcggatcatc aatagtcttc gcaacggcga tagcctgcgg gtcgcgatcg atcgccagca
    32101 aacgcccctc ttcgccaagc tgcgagagga tcagacgtga gtgaccaccg cgaccaaaag
    32161 tcccatcaat gtagatgcca tcaggacgga tattgaggcc attaacggct tcatccagca
    32221 gcaccgtagt atgtttatag ttttccatca ttttatagag acaagtcctg cagtcgctcc
    32281 gataagtctc cagtagccaa ctgctctgcg tcgatatctt ccttgacctg ttgatgccag
    32341 gttgtttcat cccacagctc aaacttgttg aactgtccaa ccagcatcac ttcttttgtc
    32401 agcccggcat gttgccgcag tactggcgcg attaacaatc gacctgcgcc atccatctga
    32461 cattcgctgg catgacctaa cagtaggcgc tgcacacggc gctcaaccgg gttcatgctc
    32521 gacagacgcg ataatttttg ctcgataatt tcccattcag gcagggggta aagcagcagg
    32581 cacgggtgat gaatgtcaat ggtgcaaacc atttgaccgg cagcgttctc aagcagctgt
    32641 tcccgataac gggtaggcac tgataagcgc cctttgctgt cgagattgac taacgttgct
    32701 ccccggaaca tgccagcctc accccttatt cccactttgc cccacaattt cccactgaaa
    32761 ggagtttacg gagcggagga aaagcttgtc aagccagtca caaggcatac cgaggtaaaa
    32821 aacccgatgt ttacggctaa tatcagcgtt gtttatttta cgtccaacgt gcgaagcaaa
    32881 ttaacgttat gaatagctgt aagaaaaaat ccctcatcgt catcaccgtt ttaaaatgac
    32941 tcactatttt ccacagcaaa tataaagtgt cagtttgcga cgcgagcggc attttaggac
    33001 atatcttccc cggttaacag tccctgttgc gtctgtgtgg cgcttgatgc cagcgggttt
    33061 gcgcaaaagc aaagaaaagt gtttgttaaa tcggcaattc atctctgcat gtaacaaaat
    33121 aatgcaaaaa cgcgctatcg atatcactta attattgtga atttcagctg gtgaggtaat
    33181 taacgggcag gtttaaaagc atttatcaat aatagccagg aatagtctta tttactttaa
    33241 ggatattgat gtccattccc gtactcaacg cgccagagag aaattcacct ggcgcgtatt
    33301 tttgttcgcg gcttagctac ggctgagcac gccgcggcga tagagattac gtttaatgcg
    33361 cgttaaacca ggttttggct tacgcggttc gtccaggctt gccaggacaa tctccagcac
    33421 acgctctgcg acatcgcggt gacgttgagc cactgccagc accgggcact gtaagaagtc
    33481 gagcagttcg ttatcgccaa aggtggcaat tgccaggtca gaaggcagtt tgccgtcacg
    33541 acgtagcgtg acatccatca ccccttgcaa caacgcaaac gacgttgtga acagtgcctg
    33601 cggcatcgga tgcgtttcca gccatttttc gaataactgg gcagccgcct cccgctcata
    33661 gctgttggca taaaggaaat gcacttcgcg aggatcatct ttccaggcag tacggaaacc
    33721 ttgttcacgc aggaagctga cagaaagctc cggcagcgcg ccaagataaa gtaccgtctc
    33781 ggcgggaaac ttacgtaact cttccgccag catttcggca tcatcctgat cggcaccaac
    33841 cacgctggtg aagtgttcac gatcgagggc gcggtccagc gcgacaatcg ggaacgggtc
    33901 gttagcccag cgttgataaa aaggatgctc aggtggcaac gacgtcgaaa caataatggc
    33961 atcaacctga cgctgtaaaa gatgctcgat acaacgcatt tcgttgtcgg gctgatcttc
    34021 cgagcaggca atcagcagtt gataacccct ttgccgcgcc tggcgttcaa gatagttagc
    34081 gatgcgggta tagctggtgt tctccagatc ggggatcaca agaccaatag aacgtgtgcg
    34141 tccagcacga agcccagctg ccacggcgtt cgggtggtaa ttgtgctcac gcaccacagc
    34201 catgactttt tcaacggttt tgtcgctcac acggtattgc ttcgctttgc cgttaataac
    34261 atagcttgca gtggtccgcg acactcccgc cagccgagcg atttcatcca gtttcacaat
    34321 tgccccttgc gtaaaatgta aaaaccatag ccattgtact ggtatgggtt aaattcatta
    34381 acatctaagc gcagaaaatt aactgcggca actgctttta ttccaggttc ccactgattt
    34441 cgcaaaaaaa gcccaacgtg acatgttggg ctgcaaattg cgcattgaga tcattctcaa
    34501 cgcattattt tatcgccgcg cgaaagtccg actacaccag agcgagcaac ttccacaatt
    34561 ttcgccacat cgcgaatcga tgctaaaaat gcatcaagct tatcgctggt gcctgctaat
    34621 tgaacggtat aaagcgaggg tgtgacatcg ataatttgcc cacggaatat ttccgtatta
    34681 cgtttcactt catcacgccc gtaaccgctg gcctgaattt tcaccagcat gatttcccgc
    34741 tcaacgtgcg cgccctgccc caactcgctc acgcgcaaga catcgaccag cttgtgtaat
    34801 tgcttttcga tttgctcaag cactttttca tcgcccacgg tctggatggt catacgcgat
    34861 aatgttggat cgtcggttgg cgcaacggtc aggctttcaa tgttgtagcc acgctgggaa
    34921 aaaaggccaa tcacgcggga taacgcgcct gattcatttt cgagtaagac tgataatatc
    34981 cggcgcataa tcaggttctc tccgttttgc ttaaccacat ttcatccatt ccgcccccgc
    35041 gaatctgcat cgggtagacg tgctcactgc catcgacggt gacatcaaca aacaccaggc
    35101 gattattgcg cacctgttcc agcgcctcgc taagtttgct ttccagttct tgcggatgag
    35161 agatctggat cccgacgtga ccataggctt ccgccagacg aacgaaatcg ggtagtgact
    35221 gcatataaga ttgtgaatga cggccggaat agatcatgtc ctgccactgt ttcaccatcc
    35281 ccagatagcg gttattgaga ttcaccacca gtaccggcaa ctcgtattgc aacgctgtag
    35341 acagttcctg gatgttcatc tgaatactgc cgtcgccagt gacgcaaacc acggtttctt
    35401 ctggcaacgc cattttgacg cccagtgccg caggtaaacc aaaacccatc gtgccgaggc
    35461 cgccagagtt aatccagcga cgcggtttgt cgaatggata ataaagcgca gcaaacatct
    35521 ggtgctgccc gacatcggac gtcacgtaag cgtccccatt agtcaaccgc caaagagtct
    35581 cgatcaccgc ctgcggttta atcttttcac tgtgagtgtc atatttcagg cactgacgag
    35641 cgcgccactg ttcaatctgc tgccaccagt cgcggatctc atccagcggt tgatggacgg
    35701 attcttgcga caggagttca agcatttgtt cgaggacctg gcgagcatcc cccacaatcg
    35761 ggatatcagc agtcacggtt ttagaaatgg aagtaggatc aatatcgata tgcagaaccg
    35821 tggcatttgg gcagtacttc gccagattgt tcgtcgttcg gtcatcaaaa cgtaccccga
    35881 cggcaaaaat cacatccgcg ttatgcatcg tcatattggc ttcgtaggta ccgtgcattc
    35941 ccaacatgcc cagtgcctga cgatgcgttg ccggaaacgc ccccagcccc attaatgagg
    36001 aaacaacggg cagattcaac gcctccaccg tttcttttag ctgctgatga cagcccgccg
    36061 tgattgcccc accgcctaca tagacaaccg gtttttttgc cgccaccagc gtttgcagag
    36121 cgcgcttaat ttgcccttta tgcccggagg tcgtgggatt gtaagaacgc atactgaccg
    36181 actccggcca gacatagggt aatttgttcg ccggatttag aatatctttc ggtaaatcaa
    36241 cgaccacggg tccaggacga ccacttgccg ccagccagaa agcctttttc agcacctgcg
    36301 gaatgtcttc cgtttgctta accagaaaac tgtgtttaac caccgggcga gaaatcccca
    36361 ccatgtcgca ctcctgaaag gcatcgtagc ctatcaacga ggtcgctacc tgcccggaaa
    36421 ggacaactaa gggaatggaa tccatataag cggtggcaat gccagtaatc gcattggtcg
    36481 cccctggacc cgacgttacc agcacgacgc cgacttcccc ggtcgcgcgc gccagaccat
    36541 cggccatatg caccgccgcc tgctcatgac gaactaaaac atgatcgata ccacccacgg
    36601 tatgcaatgc atcgtaaata tcaaggaccg cgcctccggg ataaccgaat acttgtttaa
    36661 cgccctgatc gataagcgat cggacgacca tctcggctcc agacaacatc tccatggcct
    36721 gcctcactgt ttgacggaaa aaatgtgtaa aacacattaa ccgtccgatc ttgcttaagc
    36781 aattggcagc ctgagacata aacacggggt aataagaata aacaggagga aaggtgaaaa
    36841 gaaacgataa aatcctccat tacagaggat taaattgata ataaatcggc
//
LOCUS       DAZKGF010000024        36694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZKGF010000024 DAZKGF010000000
VERSION     DAZKGF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.024"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            83..3841
                     /gene="yfaL"
                     /locus_tag="ACHOQV_001229"
     CDS             83..3841
                     /gene="yfaL"
                     /locus_tag="ACHOQV_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFP9629627.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSGNGAAAVIDSCQGYDIKANCQ
                     ASRQSLSGNMQDWSIADGQWLIFSGMANNASGGAVFLQQSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDNT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEISIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLESVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQVLDASLFSGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAISLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTAVV
                     INPITGIGEPTSTGIKVVDFAADPTQFQNNAQFRLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVTPTPPPDPDPTPDPDPTPDPDPTPDPGPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDQAGGDGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGNLFSGHWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGVWLDSWLQ
                     YAWFNNDVSEQDDGEDHYHSSGIIASLEAGYQWLPGHGVVIEPQTQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQTRLGLHSEWSTAVGVTPTLDLNYYHDPHATEIEEDGSTISDD
                     GVKQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3981..4703)
                     /gene="ubiG"
                     /locus_tag="ACHOQV_001230"
     CDS             complement(3981..4703)
                     /gene="ubiG"
                     /locus_tag="ACHOQV_001230"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFP9629628.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVEYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            4850..7477
                     /gene="gyrA"
                     /locus_tag="ACHOQV_001231"
     CDS             4850..7477
                     /gene="gyrA"
                     /locus_tag="ACHOQV_001231"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFP9629629.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLIIQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7626..9314
                     /gene="yfaA"
                     /locus_tag="ACHOQV_001232"
     CDS             7626..9314
                     /gene="yfaA"
                     /locus_tag="ACHOQV_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFP9629630.1"
                     /translation="MSGEKKAKGWRFYGLVGLGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGSAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVNQTQQGEAQIWRREVSSRYGQYPKAQAVQPDQLMSDYFFRVSLA
                     MQNKTMLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9311..9934
                     /gene="yfaT"
                     /locus_tag="ACHOQV_001233"
     CDS             9311..9934
                     /gene="yfaT"
                     /locus_tag="ACHOQV_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFP9629631.1"
                     /translation="MRHGLLALICWLCCVVTHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9958..14472
                     /locus_tag="ACHOQV_001234"
     CDS             9958..14472
                     /locus_tag="ACHOQV_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFP9629632.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDSGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDEDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARIPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTEKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIQLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPDYWQHMLDSYAEQADNMPILHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTSDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGKLAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14473..16122
                     /gene="yfaQ"
                     /locus_tag="ACHOQV_001235"
     CDS             14473..16122
                     /gene="yfaQ"
                     /locus_tag="ACHOQV_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFP9629633.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVNSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVLVELFARYPLKKITAEKSS
                     TAVKPGVLNGRYRVTFANGNHIMFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTFQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16127..16903
                     /gene="yfaP"
                     /locus_tag="ACHOQV_001236"
     CDS             16127..16903
                     /gene="yfaP"
                     /locus_tag="ACHOQV_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFP9629634.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(16977..18161)
                     /gene="atoB"
                     /locus_tag="ACHOQV_001237"
     CDS             complement(16977..18161)
                     /gene="atoB"
                     /locus_tag="ACHOQV_001237"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFP9629635.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATKKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18192..19514)
                     /gene="atoE"
                     /locus_tag="ACHOQV_001238"
     CDS             complement(18192..19514)
                     /gene="atoE"
                     /locus_tag="ACHOQV_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFP9629636.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPIMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19511..20161)
                     /gene="atoA"
                     /locus_tag="ACHOQV_001239"
     CDS             complement(19511..20161)
                     /gene="atoA"
                     /locus_tag="ACHOQV_001239"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFP9629637.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20161..20823)
                     /gene="atoD"
                     /locus_tag="ACHOQV_001240"
     CDS             complement(20161..20823)
                     /gene="atoD"
                     /locus_tag="ACHOQV_001240"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFP9629638.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21019..22404)
                     /gene="atoC"
                     /locus_tag="ACHOQV_001241"
     CDS             complement(21019..22404)
                     /gene="atoC"
                     /locus_tag="ACHOQV_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFP9629639.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSNETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22401..24227)
                     /gene="atoS"
                     /locus_tag="ACHOQV_001242"
     CDS             complement(22401..24227)
                     /gene="atoS"
                     /locus_tag="ACHOQV_001242"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFP9629640.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24394..27243
                     /gene="rcsC"
                     /locus_tag="ACHOQV_001243"
     CDS             24394..27243
                     /gene="rcsC"
                     /locus_tag="ACHOQV_001243"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFP9629641.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPLTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27329..27979)
                     /gene="rcsB"
                     /locus_tag="ACHOQV_001244"
     CDS             complement(27329..27979)
                     /gene="rcsB"
                     /locus_tag="ACHOQV_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFP9629642.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28024..29313)
                     /locus_tag="ACHOQV_001245"
     CDS             complement(28024..29313)
                     /locus_tag="ACHOQV_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9629643.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            complement(29347..29601)
                     /gene="xisR"
                     /locus_tag="ACHOQV_001246"
     CDS             complement(29347..29601)
                     /gene="xisR"
                     /locus_tag="ACHOQV_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP9629644.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(29609..29755)
                     /locus_tag="ACHOQV_001247"
     CDS             complement(29609..29755)
                     /locus_tag="ACHOQV_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629645.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            complement(29776..30018)
                     /locus_tag="ACHOQV_001248"
     CDS             complement(29776..30018)
                     /locus_tag="ACHOQV_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629646.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKARAPAQPNELA"
     gene            complement(30011..30913)
                     /locus_tag="ACHOQV_001249"
     CDS             complement(30011..30913)
                     /locus_tag="ACHOQV_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629647.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            complement(30910..31134)
                     /locus_tag="ACHOQV_001250"
     CDS             complement(30910..31134)
                     /locus_tag="ACHOQV_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629648.1"
                     /translation="MIISFTDYGEQAKLIITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            complement(31151..31513)
                     /locus_tag="ACHOQV_001251"
     CDS             complement(31151..31513)
                     /locus_tag="ACHOQV_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP9629649.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYEDLPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
     gene            complement(31506..32372)
                     /locus_tag="ACHOQV_001252"
     CDS             complement(31506..32372)
                     /locus_tag="ACHOQV_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFP9629650.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGITVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            complement(32396..33064)
                     /locus_tag="ACHOQV_001253"
     CDS             complement(32396..33064)
                     /locus_tag="ACHOQV_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629651.1"
                     /translation="MPGWKNTTPGLISIAGLSRKDHLRREQTMTATEQLFFLTAEGIH
                     TPYGLLDINKALEMLDEGCFDEPIWHGLEIVCCISEAEEKGMTGELAPEKTIMYWRWL
                     YTAMFFSEQLTRNGWTEVLNENGGTDKGTMYRGHNGGMTIYLKPLRLAMQNYMEWALI
                     ETYGAESGLLKSILMYKAFTGALGDGEKTGITMEGYRAMHMILDGLISEATDNSEPET
                     RVFH"
     gene            complement(32967..33527)
                     /locus_tag="ACHOQV_001254"
     CDS             complement(32967..33527)
                     /locus_tag="ACHOQV_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629652.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQCLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            complement(33935..34297)
                     /locus_tag="ACHOQV_001255"
     CDS             complement(33935..34297)
                     /locus_tag="ACHOQV_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629653.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGQKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITRCVNH"
     gene            complement(34472..34672)
                     /locus_tag="ACHOQV_001256"
     CDS             complement(34472..34672)
                     /locus_tag="ACHOQV_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629654.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCMIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            complement(34669..34860)
                     /locus_tag="ACHOQV_001257"
     CDS             complement(34669..34860)
                     /locus_tag="ACHOQV_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629655.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(34862..35239)
                     /locus_tag="ACHOQV_001258"
     CDS             complement(34862..35239)
                     /locus_tag="ACHOQV_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9629656.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(35325..35984)
                     /locus_tag="ACHOQV_001259"
     CDS             complement(35325..35984)
                     /locus_tag="ACHOQV_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP9629657.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            36324..36617
                     /locus_tag="ACHOQV_001260"
     CDS             36324..36617
                     /locus_tag="ACHOQV_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629658.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
BASE COUNT         8888 a         8905 c         9568 g         9333 t
ORIGIN      
        1 cagaatttac aaaagaaaaa taccctatat cgcataatgc gattaaatac gccatcttat
       61 agaaaatgat ggttagatga atatgcggat tatctttcta cgcaaggagt atttatcttt
      121 actcccgtca atgattgcat ctcttttctc tggtaacggt gccgcagcgg tcatcgattc
      181 atgccaggga tatgatatca aagcgaactg tcaggccagc aggcaaagcc tttcgggcaa
      241 tatgcaggac tggagcatcg cggatgggca atggttgatt ttttcgggta tggccaataa
      301 tgccagcggt ggcgcagtat ttttgcagca aagtgctgaa tttacgattt caccacaaaa
      361 tgaaacaggg atgaccctgt ttgctgataa ctcggttagt ggcgaatata ataatggtgg
      421 ggctatcttt gctaaagaaa actcaacgat aaatcttgcg aatgttattt tcgacagtaa
      481 tgttgcagga ggctatggcg gagcaatcta ttctgccggt accaatgata ccggtgccgc
      541 cgatttacgt gtcactaacg ccgtatttca caataacatc gctaatgacg gcaaaggcgg
      601 tgcaatttat accatcaata atgacgtcta tttaagtgat gatgctttta ataataacca
      661 ggcatataca tcaacaagtt atagtgatgg ggatggcggc gctattgatg ttacggataa
      721 tagtacggat aacacacatc tttcaggaaa aacgataatt aataatacct cctttaccaa
      781 taactatgca gaaggttatg gcggagcaat ttataccagc agcaccacat ccccgtatct
      841 tattgatatt tctgttgatg ataattatga ccaaaacaat ggtgtgatga ttgatgaaaa
      901 taacagcgcc tcgggatatg accattcggc gacggcagcg gcggggggct ttatgtatat
      961 cggtcatagt gtggctgaat ttaacattgc cgccgataaa acgctggtaa taggcaatac
     1021 cagcaacgat ggcgcgattg attctcttgc cggaacggga gtcatcgtta aagagggagc
     1081 gggagagtta gtcctcaatg ccgacaataa tgcgtttacc ggcgagataa gtatccagaa
     1141 tggcgaggtg acgttggggc gcagcgatga gttaatgaat gtcggcgaca cgcactgtca
     1201 gagcgatccg caggattgct ttggtctgat ggtcggcagc actgttcatt ctgagtatca
     1261 ggcagaactg aatgttggca atacacaaca aacgtttgtg cactcattaa ccggttttgc
     1321 taatggcatt ttgaatatcg acgcaggcgg caatgttacc gtaaaccagg gcggtttttc
     1381 cggctcgatt cagggtgaag gacagttgac ggtagcgcaa gatggcagct atctcctgac
     1441 aggggcgcag tcgatggcat taaccggcga tattgtggtg gaagataacg ccgtattgtc
     1501 gctagcgggg aatcaggccg atttacgggc aatgcagagc gatccgcaat cgatcgtgct
     1561 aaatggcgga gtattggatc tctctgattt caccacctgg gatggcgata gctcatacaa
     1621 tgacggtctg caaatcagcg gcagcggggg gacagtgatt ggcagtaatg atgtcgttga
     1681 tatcagcagt ggcgatgatt tacatattgg cggcagtgat gccagccaga atggcgttta
     1741 tgtcgtcatt aatgcggggg atcagcgcgt aacgctggcg aataacaacg gttatctcgg
     1801 taatacgcaa atcgcctccg gtacgcttga agtcagcgat aattcgcaat tgggggacac
     1861 aagttacaac cgttcagtga tcttcacaga tccccaacaa cacagtgaaa tggatgtgac
     1921 gactgatgtt gacactcgct cggctacaac aggtcagggc aggaatattg aaatgcgcgc
     1981 tgatggcgaa atacacgttg aggatggcgt agatacgcaa tggggcgggt tgatggcgga
     2041 tagtaccggg caacaactgg agagcgtaag cacgttgact aaaagtggcg gcggtacgct
     2101 ggagttgacc gccagcggca ctgcgacgtc tgcggtacgt gtggaggacg gaacgctaaa
     2161 aggtgaagcg gagaatatta ttccttatgt ttcttcactg tgggtggggg aagacggtgt
     2221 ttttgaaacc gggcaaaatc aggatattcg ctcaatcgat gccacctctg gcggcgatat
     2281 cgacataacc gatggtactg tgttgcgatt gacgcagcag gatacgaacc aggtgctgga
     2341 tgcctcgctg tttagcggcg acggtacgtt ggtgaatgcc accgatggcg tgacgctgac
     2401 aggcgagctt aataccaacc ttgaaactga cagcctgact tatctttccg acgtgacggt
     2461 taatggcaat ctgaccaata cgtccggtgc gattagcctg caaaatggcg tcgctggcga
     2521 cacgctgacg gtaaatggtg attataccgg cggcggtacg ctactgctcg acagcgaatt
     2581 aaacggcgat gactcggcaa gtgaccaact ggtattgaac ggtaatactg ctggcaacac
     2641 ggccgtggta attaacccca ttacgggtat tggtgagccg acatctacag gtattaaagt
     2701 ggttgatttc gcagctgatc ccacgcagtt tcaaaacaat gcgcagttcc gtctggcagg
     2761 aagcggctac gtcaatatgg gggcgtatga ctatacgctg gtggaagata acaacgactg
     2821 gtatctgcga tcgcaagaag tcactccgac gccgccgccc gatccggacc cgactccaga
     2881 tcctgatccc acgccggatc ccgacccaac ccccgaccct ggacctacgc ctgcttacca
     2941 gccggtgttg aatgccaaag ttggtggata ttttaataac ctacgtgcgg caaatcaggc
     3001 atttgtgatg gagcgacgcg atcaggctgg tggcgatggt cagacgctga atttacgcgt
     3061 tatcggaggg cgttatcatt acacggcggc agggcagttg gctcagcatg aagacacttc
     3121 tacggtgcag cttagcggca acctgtttag cgggcactgg ggtgatgatg gcgagtggat
     3181 gcttgggatt gttggtggct acagcgataa ccagggcgac agccgctcga atatgaccgg
     3241 aactcgcgcc gataaccaga accacggtta tgccgttggg ctgacctcaa gctggtatca
     3301 gcacggtaat cagaagcagg gggtctggct ggatagctgg ctgcaatatg cgtggtttaa
     3361 caatgatgtt tccgaacaag acgatggcga ggatcattac cactcgtcgg ggattatcgc
     3421 ctcgctggaa gccggttatc agtggttacc ggggcatggt gtggtgattg aaccgcagac
     3481 gcaggtgatt tatcagggcg tgcagcagga tgattttacc gccgctaacc atgcgcgcgt
     3541 gtcacaatcg cagggtgatg atattcagac gcggctgggt ttacacagcg aatggagtac
     3601 tgctgttggt gtcacgccaa cgttagatct gaattattat cacgatcccc atgcgacgga
     3661 aattgaagag gatggcagca ctatcagtga cgatggggtg aagcaacggg gtgaaataaa
     3721 agtgggagtc acgggcaata tcagtcagcg agtgtccctg cgcggcagcg tggcgtggca
     3781 gaaagggagt gatgattttg cccagacggc agggtttttg tcgatgacgg tgaaatggta
     3841 atcgctgtct gaccggcact tttgccggat gcggcgtaaa cgccttatct ggcctacggg
     3901 agcatttgta ggcctgataa gacgcggcaa gcgtcgcatc aggcattggc acggttgtcg
     3961 gatgaatcca aacaacaacc tcacttattc tgcgtgtgca gcatatagtt cacatccacg
     4021 ccggggccga gcttaaagga attagtgatc gggttgtaat gcagcccggt catatgccgc
     4081 tcttttaaac tggtctgatc cacccagccc agcaattctg ccggtttaat aaacttcttc
     4141 acatcatgcg tgcctttggg caccatgcgc aaaatatatt cagcaccaac caccgccatc
     4201 agccatgact tgccgttgcg gttaagcgtc gagaaaaaga catcgccgcc tggtttcacc
     4261 agttgcgcac aggctctgac cactgactgc ggatcgggga cgtgctccag catctccatg
     4321 caggtcacta catcatactg cccggcatgt tttgccgcgt gctcttccac ggtttcctgt
     4381 acgtactcta cctgaatgcc gctttccagc gcgtgcaact ttgccacctg caatggctca
     4441 aagcccatat ccagaccggt caccgtcgcg ccttcgcgcg ccatactctc ggccagaatg
     4501 ccgccgccac aaccgacatc gagcaccttt ttgccaaata aaccgccagc acgctcggca
     4561 atatagccca gacgcagcgg gttaatgcgg tgcagcggtt tgaactcacc ttcaagatcc
     4621 caccagcggg aggcgacagc ttcaaattta gcgatctctt cgtggtctac gttatggttt
     4681 accggcgatt tttcggcatt cattggcact tctactccgt aattggcaag acaaacgagt
     4741 atatcaggca ttggaggtga ataaagcgta taggtttacc tcaaactgcg cggctgtgtt
     4801 ataatttgcg acctttgaat ccgggataca gtagagggat agcggttaga tgagcgacct
     4861 tgcgagagaa attacaccgg tcaacattga ggaagagctg aagagctcct atctggatta
     4921 tgcgatgtcg gtcattgttg gccgtgcgct gccagatgtc cgagatggcc tgaagccggt
     4981 acaccgtcgc gtactttacg ccatgaacgt actaggcaat gactggaaca aagcctataa
     5041 aaaatctgcc cgtgtcgttg gtgacgtaat cggtaaatac catccccatg gtgactcggc
     5101 ggtttatgac acgatcgtcc gtatggcgca gccattctcg ctgcgttaca tgctggtaga
     5161 cggtcagggt aacttcggtt ccatcgacgg cgactctgcg gcggcaatgc gttatacgga
     5221 aatccgtctg gcgaaaattg cccatgaact gatggccgat ctcgaaaaag agacggtcga
     5281 tttcgtagat aactatgacg gcacggaaaa aattcccgac gtcatgccaa ccaaaattcc
     5341 taacctgctg gtgaacggtt cttccggtat cgccgtaggt atggcaacca acatcccgcc
     5401 gcacaacctg acggaagtca tcaacggttg tctggcgtat atcgatgatg aagacatcag
     5461 cattgaaggg ctgatggaac acatccccgg gccggacttc ccgacggcgg caatcattaa
     5521 cggtcgtcgc ggtattgaag aagcttaccg taccggtcgc ggcaaggtat atatccgcgc
     5581 ccgtgctgaa gtggaagttg acgccaaaac cggacgtgaa accattatcg tccacgaaat
     5641 tccgtatcag gtaaacaaag cgcgcctgat cgagaagatt gcggaactgg taaaagaaaa
     5701 acgcgtggaa ggcatcagcg cgctgcgtga cgagtctgac aaagacggta tgcgcatcgt
     5761 gattgaagtg aaacgcgatg cggtcggtga agttgtgctc aacaacctct actcccagac
     5821 ccagttgcag gtttctttcg gtatcaacat ggtggcattg caccatggtc agccgaagat
     5881 catgaacctg aaagacatca tcgcggcgtt tgttcgtcac cgccgcgaag tggtgactcg
     5941 tcgtactatt ttcgaactgc gtaaagctcg cgatcgtgct catatccttg aagcattagc
     6001 cgtagcgctg gcgaacatcg acccgatcat cgaactgatc cgtcatgcgc cgacgcctgc
     6061 agaagcgaaa actgcgctgg tggctaatcc gtggcagctg ggcaacgttg ccgccatgct
     6121 cgaacgtgcg ggcgatgatg ctgcgcgtcc ggaatggctg gagccagagt tcggcgtgcg
     6181 tgatggtctg tactacctga ccgaacagca agctcaggcg attctggatc tgcgtttgca
     6241 gaaactgacc ggccttgagc atgaaaaact gctcgacgaa tacaaagagc tgctggatca
     6301 gatcgcagaa ctgctgcgta ttcttggcag tgccgatcgt ctgatggaag tgatccgcga
     6361 agagctggaa ctggttcgtg aacagttcgg tgacaaacgt cgtactgaaa tcaccgctaa
     6421 cagcgccgac atcaacctgg aagatctgat catccaggaa gatgtggtgg tcacgctttc
     6481 tcaccagggt tacgtgaagt atcagccgct ttctgaatat gaagcgcagc gtcgtggcgg
     6541 gaaaggtaaa tctgccgcac gtattaaaga agaagacttt atcgaccgac tgctggtggc
     6601 gaacacccac gaccatattc tgtgcttctc cagccgtggc cgcgtctatt cgatgaaggt
     6661 ctaccagtta ccagaagcca ctcgtggcgc gcgtggtcgt ccgatcgtca acctgctgcc
     6721 gctggagcag gacgaacgta tcactgcgat cctgccggtg accgagtttg aagaaggcgt
     6781 gaaagtcttc atggcgaccg ctaacggtac tgtgaagaaa accgtcctca ccgagttcaa
     6841 ccgtctgcgt accgccggta aagtggcgat caaactggtt gaaggcgatg agctgatcgg
     6901 cgttgacctg accagcggcg aagacgaagt aatgctgttc tccgccgaag gtaaagtggt
     6961 gcgctttaaa gagtcttctg tccgtgcgat gggctgcaac accaccggtg ttcgcggtat
     7021 tcgtttaggc gaaggcgata aagtcgtctc tctgatcgtg cctcgtggcg atggcgcaat
     7081 cctcaccgca acgcagaacg gttatggtaa acgtaccgca gtggcggaat acccgactaa
     7141 gtcgcgtgcg acgaaagggg ttatctccat caaggttacc gaacgtaacg gtttagtggt
     7201 tggcgcggtg caggtagatg actgcgacca gatcatgatg atcaccgatg ccggtacgct
     7261 ggtacgtact cgcgtttcgg aaatcagcat cgtaggccgt aacacccagg gcgtgatcct
     7321 catccgtact gcggaagatg aaaacgtagt gggtctgcaa cgtgttgctg aaccggttga
     7381 cgaggaagat ctggatacca tcgatggcag tgccgcggaa ggggacgatg aaatcgctcc
     7441 ggaagtggac gttgacgacg agccagaaga agaataattt tacttcttca tgccaaaagg
     7501 gagctatctc ccttgtttga attgaaaagt ccaggctgca aagtctgggc ttttgtcgta
     7561 ttagggcgcg gtaaagtttt gctgtgctcg taaaaaatgg ctggctttac acaaggaatg
     7621 tggcaatgag tggtgaaaaa aaggcgaaag gctggcggtt ctatggtctt gtaggtttgg
     7681 gcgcaatagt actgctttcc gctggcgtct gggcgttgca atatgctggc agtgggccag
     7741 aaaaaacgtt gtcgccgctg gtggtgcaca acaatctgca aatcgatctc aatgagccgg
     7801 acctctttct cgacagcgac tctctgagcc agcttcccaa agacctcctt accattccgt
     7861 ttctccacga tgttctgagt gaagatttcg ttttctatta tcagaatcat gccgatcgtc
     7921 tgggcattga aggcagcatt cgccgcattg tctatgaaca cgatctcacg ctgaaagata
     7981 agctcttttc gtcactctta gatcagcccg cgcaggcggc gctatggcac gataaacaag
     8041 gccatctttc acattacatg gtgctgatcc agcgcagtgg tttaagcaaa ctgttggagc
     8101 cattgttgtt tgccgctacc agcgacagcc agttaagcaa aacggaaatc agtagtatta
     8161 agctaaatag tgaaaccatc ccggtttatc agttgcgcta taacggcaat aacgccctga
     8221 tgttcgcgac ttatcaggac aagatgctgg tgttttccag tacggatatg ctgtttaaag
     8281 atgaccagca ggataccgaa gccacggcga tcgcaagtga tttgttgagc ggtaaaaaac
     8341 gctggcaagc cagctttggc ctggaagagc gtgctgctga aaaaacgcca gtacgccagc
     8401 gtattgttgt cagcgccagg ttgctggggt ttggctacca gcgattaatg ccttcttttg
     8461 ctggcgtacg cttcgaaatg agcaacgacg gctggcatag ctttgtggcg ttaaatgatg
     8521 aatccgccag cgtagatgcc agtttcgatt ttacgccggt atggaacagt atgcctgccg
     8581 gggccagctt ctgtgtggcg gtgccgtatt cgcatggtat tgccgaagag atgctttcgc
     8641 acatcagcca ggaaaacgac aagctgaacg gggcgttaga tggtgcagcc gggctgtgct
     8701 ggtatgaaga ctcaaaatta caaaccccgc tatttgttgg tcaatttgat ggctcagccg
     8761 aacaggcaca actgcctggg aaactgttta cgcaaaatat tggtgcgcac gaaagcaaag
     8821 cgccagaagg tgttttgccg gtaaaccaga ctcagcaggg cgaagcgcaa atctggcgtc
     8881 gcgaagtgag ttcccgatac ggccagtatc cgaaagcgca ggcggtgcaa cccgatcaat
     8941 taatgtcgga ttattttttc cgcgtgtcgc tggcgatgca aaacaagacg atgcttttct
     9001 ccctcgatga cacgctggtt aataacgcgc tgcaaacact gaataaaacc cgcccggcaa
     9061 tggtggatgt gatacccact gacggcatcg ttccgctcta tatcaatcca caaggcgtgg
     9121 cgaaactgct gcgtaacgaa acgctgacca gtttgccgaa gaatctcgaa ccggtttttt
     9181 ataacgccgc acaaacttta ttaatgccga agctggacgc tttatctcaa caaccgcgtt
     9241 atgtcatgaa gctggcccag atggaacccg gtgccgcctg gcagtggcta cccataacct
     9301 ggcaaccgct atgagacacg ggctgctggc gctgatttgc tggctgtgtt gtgttgttac
     9361 ccatagcgaa atgctgaatg tcgaacaatc cgggctgttt cgcgcctggt ttgtacgcat
     9421 tgcacaggaa cagctccgcc agggtccaag tccacgctgg tatcagcagg attgtgcggg
     9481 cctggtgcga tttgcagcga acgagacgct gaaagttcac gacagtaaat ggctaaaaag
     9541 taacgggtta tctaaccagt atttgccgcc agagatgacg ctaacacctg aacagcggca
     9601 actggcgcaa aactggaatc aggggaacgg gaaaaccggc ccctacgtga ccgcaattaa
     9661 tttgattcag tacaacagcc aatttattgg ccaggacata aaccaggcgc tgcctggcga
     9721 tatgattttt ttcgatcagg gcgatgccca gcacttaatg gtctggatgg ggcgttacgt
     9781 catctaccac accggaagcg ccacgaaaac tgacaacgga atgcgcgcag tcagtctgca
     9841 acaacttatg acatggaagg acacccgatg gatacccaac gattccaatc ccaatttcat
     9901 tggcatttat cgtttaaatt ttctggcgcg atagcagcgt gcttgttcct gtctcttgtg
     9961 ggaacagggc ttgccaatgc tgatgattcg cttccttcca gcaactatgc gccgcccgcc
    10021 gggggaacat tctttttgct cgctgacagc agttttagca gcagtgaaga ggcgaaagtg
    10081 cgactggaag cgccggggcg tgattatcga cgctatcaga tggaagagta cggcggcgtg
    10141 gacgttcgcc tgtatcgtat tcctgacccg atggcatttt tgcgccagca gaaaaacctg
    10201 catcgcattg tggtgcaacc gcaatatttg ggcgacgggc tgaacaatac gctgacctgg
    10261 ctgtgggata actggtacgg caaatctcgc cgcgtgatgc agcgtacttt ttcttctcag
    10321 tctcggcaga atgtgactca ggcgttaccc gaattacagc tcggtaatgc cattattaaa
    10381 ccttcccgtt atgtacagaa caaccagttt tcaccgctga aaaaatatcc cctggtggaa
    10441 cagttccgtt acccattatg gcaggctaaa ccggtcgagc cgcagcaagg ggtaaaactg
    10501 gaaggcgcat ccagcaattt catctcgccg cagccgggca acatctatat tccgctaggc
    10561 aagcaagagc cggggctgta cctcgtcgag gcgatggtag gtgggtatcg ggcgacgacg
    10621 gtggtgtttg tttccgatac cgtggcgctt agcaaagtgt caggtaacga gcttctggtg
    10681 tggaccgcgg gtaaaaaaca gggggaagcg aagccgggat cagagatctt gtggactgac
    10741 ggtcttggcg tgatgacccg cggtgtgacc gatgacagcg gtaccttgca gttacaacat
    10801 atatcgccgg aacgttcata cattctgggt aaagatgctg aaggcggcgt ttttgtctcc
    10861 gagaattttt tctacgaaag cgaaatctac aacacccgct tgtatatctt taccgaccgc
    10921 cctctgtatc gtgcaggcga tcgtgttgat gtcaaagtga tgggccgcga gttccacgac
    10981 ccgttgcatt catcccccat cgtcagcgcc ccggcgaagc tttcagtgct ggacgccaac
    11041 ggcagtctgt tgcaaaccgt caatgtcacg cttgatgcgc gtaatggcgg gcaggggagt
    11101 ttccgcctgc cagaaaatgc cgtcgccgga ggttatgagt tacgcctggc ttaccgcaaa
    11161 caggtctata gcagcagttt tcgcgtggca aactacatca agccacattt cgagattggt
    11221 ttagctctcg acaaaaaaga gttcaaaaca ggcgaagcgg tgagcggcaa actgcaactg
    11281 ctttacccgg atggagagcc ggtaaaagat gcccgcgtgc agttaagttt gcgcgctcag
    11341 caattatcaa tggtcggtaa cgatttgcgt tatgccggac gttttcccgt gtcgctggaa
    11401 ggcagcgaaa cggtgtccga tgatagcggt catgtggctt taaatctccc cgccgccgat
    11461 aaaccgagcc gctatttgtt aaccgtctcc gccagtgacg gcgcggcgta tcgcgtcacc
    11521 accaccaaag agatcctcat tgaacgcggc ctggcgcatt actctttaag taccgccgca
    11581 caatacagta atagcggcga gtcggttgtg ttccgttatg ccgcgctgga atcttcaaaa
    11641 caggttcctg ttacgtatga atggttgcgt ctcgaagacc gcacgagcca tagcggagat
    11701 ctaccgtcag gcggcaaatc ctttaccgtc aatttcgata aacctggcaa ctacaatctg
    11761 acattacgcg atgaagacgg cttaattctc gccgggttaa gccatgcagt cagcggtaag
    11821 ggcagcacgg cgcatacggg tacggtagat atcgtggcgg ataaaacgct gtaccagcca
    11881 ggcgaaaccg cgaagatgct gattaccttc ccggagccaa ttgatgaagc attattgacg
    11941 ctggaacgcg atcgcgttga acagcagtcg ctgctatcgc atccggcaaa ctggctgacg
    12001 ttacaacgtt taaacgatac ccagtatgaa gcccgcatac cagtgagcaa ttcctttgcg
    12061 cctaacatca ctttttcggt gctgtatacc cgtaacggtc agtacagttt tcagaacgcc
    12121 gggatcaaag ttgccgttcc ccagctggat atccgggtga aaacggaaaa aacccattac
    12181 cagcctggtg aactggtcaa tgtcgaatta acctcgtcgc tgaaaggtaa acctgtttct
    12241 gcgcagctaa cggttggtgt ggtcgatgaa atgatctacg cgctgcaacc ggaaatcgcg
    12301 ccgaatatcg gcaaattttt ctatccgctg gggcgtaaca atgtgcgtac cagctccagt
    12361 ctgtcgttta tcagctacga ccaggcgctc tccagcgagc cggttgcgcc tggcgcgact
    12421 aaccgcagcg agcggcgagt aaaaatgctt gaacgtccac ggcgtgaaga ggtggatacc
    12481 gcggcatgga tgccgtcact cacaaccgat aaacaaggca aagcgtattt cacgttcctg
    12541 atgcctgatt cgttaacccg ctggcgcatc accgcgcgtg ggatgaacgg cgacgggctg
    12601 gtcgggcagg ggcgtgctta tctgcgttcg gaaaaaaatc tctacatgaa gtggagtatg
    12661 ccaacggtgt atcgcatggg cgacaaaccg gcagcaggac tgtttatctt cagtcagcag
    12721 gataacgaac cggtggcgct ggtgactaaa tttgcaggtg ctgagatgcg ccagacgctg
    12781 acgctgcaca aaggggcgaa ttatatttcg ctgacgcaga atattcagca atctggcttg
    12841 ttaagtgcag aactgcaaca aaatgggcaa gtacaggaca gcattagcac aaaactgtct
    12901 tttgtggata acagctggcc cgttgaacag cagaaaaacg tcatgctcgg cggtggcgat
    12961 aacgcgctga tgttgcccga gcaggcgagc aatatccagc tacaaagtag cgaaacgccg
    13021 caggagattt tccgtaacaa tcttgatgcg ttagtcgatg aaccgtgggg gggcgtgatc
    13081 aacaccggta gccgtctgat cccgctcagt ctcgcctggc gttcacttgc cgatcatcaa
    13141 agtgccgccg ctaacgacat tcgtcagatg attcaggata accgtctgcg gctgatgcaa
    13201 ctggcggggc ccggagcgcg ctttacctgg tggggtgaag atggcaatgg cgacgccttc
    13261 ctgacggcat gggcatggta cgccgactgg caggccagcc aggcgctcgg cgtaacgcag
    13321 caaccggatt actggcaaca tatgctcgac agttatgccg agcaggcaga taacatgccg
    13381 atattgcatc gcgcgctggt gctggcgtgg gcacaggaga tgaatctacc gtgcaaaacg
    13441 ttgttgaaag ggttggatga agctatcgcc cggcgcggaa ctaaaactga agatttctct
    13501 gaggaagaca ccagcgatat caatgacagc ctgatcctcg atacaccgga atctccactg
    13561 gcagatgcgg tggcaaacgt cttaaccatg acgttgctga aaaaagcgca gttgaagtcc
    13621 acggtgatgc cacaggttca gcaatatgcg tgggataaag cggcaaacag caatcagccg
    13681 ctggcgcaca cggttgtgct gctcaatagc ggtggtgacg ctacccaggc ggccgctatt
    13741 ttaagtggtt tgaccgctga gcaatccact attgagcgcg cgctggccat gaactggctg
    13801 gcgaaatata tggcgacaat gcctccagtt gttttgcctg cgcctgcggg cgcatgggct
    13861 aaacataagt taactggagg gggcgaagac tggcgttggg ttggtcaggg tgtgccggac
    13921 attctctctt ttggtgacga attatcgccg caaaatgtgc aggtccgctg gcgtgaaccg
    13981 gcaaaaacgg ctcaacaaag taacattccg gtgaccgttg aacgccagtt gtatcggctt
    14041 atccccggtg aagaagagat gagctttact ctgcagccgg tgaccagcaa tgagattgac
    14101 agcgatgcgc tgtatctcga tgaaattacg cttaccagcg agcaggatgc cgttctgcgc
    14161 tacggtcagg tggaagtacc gctcccgcca ggggctgacg ttgagaggac tacgtggggc
    14221 atttcggtca ataaaccgaa cgctggaaaa cagcaggggc agttgctgga aaaagcgcga
    14281 aatgaaatgg gcaaactggc ctatatggtg ccggtgaaag aactgacggg aacggtcact
    14341 ttccgccatt tgctgcgctt ctcgcaaaaa gggcaattcg ttctgcctcc tgctcgttat
    14401 gtgcgttcct atgcacctgc acagcaaagt gttgcggcag ggagcgaatg gaccgggatg
    14461 caggtgaaat aagtgaactg gcgcagaatc gtctggctat tggcgttggt tactctgcca
    14521 acgcttgcgg aggagacgcc gttacaactg gtgctgcgcg gtgcgcagca cgatcaactg
    14581 tatcagctgt cgtcatcagg cgtgaccaaa gtctcggcat tacctgacac gctcaccacg
    14641 ccgttaggga gtttgtggaa actgtatgtt tacgcctggc tggaggatac ccatcaaccg
    14701 gagcagccct accagtgtcg cggaaactcc ccggaagagg tttattgttg tcaggctggg
    14761 gagagtataa cgcgcgatac cgcgctggta cgttcctgtg ggctctattt tgcgccgcag
    14821 cggttgcata ttggcgcaga tgtgtggggg caatattggc aacagcgtca ggcacctgcg
    14881 tggttagcgt ctcttactac gctgaagcca gaaacctcgg taacggttaa ctcgctgctt
    14941 gattcattag cgacgttacc tgcgcaaaac aaagctcagg aagtcttact ggatgtggtg
    15001 ctggacgagg cgaaaatcgg tgtcgcatcc atgctcggca gtcgggtacg ggtgaaaacc
    15061 tggagctggt ttgccgatga taaacaggaa atccgtcagg gtggttttgc cggctggtta
    15121 accgatggta caccgctgtg ggtgactggc agtggcacca gcaaaacggt actgacccgt
    15181 tatgcgacgg tgcttaatcg ggttttgcca gtgccgacgc aagttgccag tgggcaatgt
    15241 gtcctggtgg agctgtttgc ccgctatccg ctgaagaaaa tcacggcgga aaagagttcg
    15301 acggcagtaa aacccggcgt acttaatggg cgctatcgtg tgacgtttgc caatggtaac
    15361 cacattatgt ttgtcagcca cggtgagacg accttactga gcgaaaaggg caaactgaaa
    15421 ttgcagtcgc atctcgatcg tgaagagtac gttgccagag ttctggatcg tgaggcgaaa
    15481 agtacgccac ctgaggcagc gaaagccatg accgtggcga tacgcacttt tttacagcaa
    15541 aacgccaatc gggaaggcga ttgcctgacc atccctgaca gcagcgccac gcagcgtgtt
    15601 tctgcttcgc cagctaccac gggggcgcga acgatgaccg cctggacgca ggatcttatc
    15661 tatgcgggcg atccggttca ttatcacggc agtcgcgcta ccgaagggac gctttcctgg
    15721 cggcaagcca cggcccaggc ggggcaggga gagcgttacg atcagatact cgcgtttgct
    15781 tatcccgaca atagccttag ccgctgggga gcgccacgct caacctgcca gttattaccc
    15841 aaagcgaaag cctggctggc gaaaaaaatg ccgcagtggc ggcgtatatt acaaggtgag
    15901 acggggtaca acgaaccaga cgtgtttgcg gtttgccgtt tagtctcagg tttcccctat
    15961 accgatcggc agcagaaacg gctgtttatt cgtaacttct ttacgtttca ggatcggctg
    16021 gatttaaccc atgagtatct gcacctggcc tttgatggtt atcccaccgg gcttgatgag
    16081 aactatatcg aaacgcttac ccgacaatta ttaatggact aatgctatgc gaaagatttt
    16141 tcttccgttg ttactggtgg cgctttcgcc tgtcgctcac agtgaaggtg tgcaggaagt
    16201 cgagattgat gcaccgctct ctggctggca tccggcagag ggtgaagatg ccagtttttc
    16261 acaatcgatc aattacccgg cgtcgtccgt caacatggca gatgatcaaa atatctcagc
    16321 gcagatccgc ggcaaaataa agaattatgc tgcggcgggt aaagttcagc agggccggct
    16381 ggtggtcaac ggtgccagta tgccgcagag aattgaatcc gatggttcat ttgcacgccc
    16441 ttatattttc acggaaggca gcaacagcgt gcaggtcatc agcccggatg ggcaaagccg
    16501 gcaaaaaatg cagttttact caacgccggg tacaggaacg attcgtgcac gtttacggct
    16561 ggttctctcg tgggatacgg acaataccga tctcgatctc catgttgtca cgcccgatgg
    16621 cgaacacgcc tggtacggta acacggtgct gaaaaacagt ggtgcactgg atatggatgt
    16681 cacgacgggg tacggacccg agattttcgc catgccagcg ccgattcacg gacgttatca
    16741 ggtgtatatc aactactatg gcggacgcag cgaaacggaa ttgacgaccg cccaactgac
    16801 gctgatcacc gatgaaggat cggtcaatga gaaacaggaa acatttattg taccgatgcg
    16861 taatgctggc gagctgacgc tggtgaaaag ttttgactgg tgattaatgg ggttaccttc
    16921 gccgtcgatg ttcaagaaaa cacccgataa ctttcgctat cgggtgtttt tattgattaa
    16981 ttcaaccgtt caatcaccat cgcaattccc tggccgccac caatgcatag tgttgccagc
    17041 cccagcgttt tatcgcgtgc ctgcatggca tgtaatagtg tgaccagaat acgagcacca
    17101 ctggcaccga taggatgccc gagcgcgatg gccccgccgt tgacattcac tttctcagaa
    17161 tcaaagccca gggttttccc aacggcaagg aactgtgcag caaatgcttc attagcctca
    17221 atgagatcaa tatccgccag ttgcagcccc gccagttgta acgctttttt cgtggcaggt
    17281 actggcccca tacccatcaa tgcggggggc acgccaccgc tggcataact tttaatgcga
    17341 gccagggggg taaggcctgc tgccagcgcc gcagattctt ccataatcac cagagcggca
    17401 gcaccgtcgt taataccaga cgcgttcccg gcggtgactg ttcctgcttt atcgaaggcc
    17461 gggcgcaatg cgcctaacgc ttcagtcgta gaatccgctt tcgggaattc gtcttgactg
    17521 aagacgaagg ttttcttccg agtgacaaca tttaccggga cgatttcggc tgtaaaagca
    17581 ccggactcaa ttgcggctgc cgctttacgc tgtgaatgta gcgccagttc atcctgcatt
    17641 tcacgggtaa ttccgtactc tttagccacg ttttcggcgg taatccccat atgataacca
    17701 tgggtggcgc acatcagacc atcgcgcagg attacgtcat aaacctgtcc gtctccaaga
    17761 cgataaccag agcgtgcttt tgcatcgagt aagtagggcg ctaaactcat attttccata
    17821 ccccccgcca caatgctctg cgcctgacct gcctgaattg cctgggcggc aagcgccaca
    17881 cttttaagac ccgaaccaca cactttattg accgtgaatc cgcacaccgt ttcagccagt
    17941 ccgcttttta gcagcgcctg acgtgccgga ttttgcccca gtccggcttg caacacgtta
    18001 cccataatca cttcatcaac gtgtagtgaa tcgatttttg cacgttcaat ggcggcttta
    18061 attactgtcg cccccaggtc gatggcgctg gtggaagcga gtgaaccgtt aaaactaccg
    18121 atagcagtac gtaccgcact gacgatgaca caatttttca ttttatattc cttctgtctg
    18181 tagggttgcc gtcagaacag cgttaaacca atgacgaaaa tgacgccgga gaagagcaag
    18241 gcagtgatgc aatagcccat gatgtcgcgg acaccgagtc cggcgattgc cagtgctggc
    18301 agcgcccaga acggttgtgc catgttcatc cattgctcgc cgtaggcgat cgccattacc
    18361 gatttaccga gatcagcgcc cagcgcctgg gctgcgggca tcacgaacgg cccctgaata
    18421 acccagtgcc cgccgccaga cggaacggcg aagttaatca gtgcagaact aaaaaaggtc
    18481 atgatcggga aggtgtcttt gttcgcaaca ttgatgaaga attcggtaat gagtccgccc
    18541 agaccggaat gctccatcat cagttggatc ccagcgtaga aggggaattg caccagaata
    18601 ccggcagtac tgcgtgctgc cgcgctgata gcacgcatat aagccattgg cgttttatgt
    18661 agcagcagac ccgcaatcat aaacatcagg ttgacggtat tgatggtgat gttgaagcca
    18721 tgttcgctga agtacatcgc aaggtaagcg ataccgagtg cgccgatgat caacgtcaga
    18781 atgcggcttt cttccagtcg ctcggatggt ggggcatctt tcggtagctg cttttgaaaa
    18841 tcagcctctt ccatgagtag tttcggatcg atactcacca cgtcagacgg ttttggcatc
    18901 atcatgcggg tgataaatgg catcaccaca atcaacgcca cagtgatgaa aatattaaaa
    18961 ccactgaaca gagtatcgcc caccgggatc agcccggcga tatgctcaac cgggttgccc
    19021 ggtgttgcag ccaacagagg cattgagcca gagaaaccgc caccccaggt gagaaaacca
    19081 atgtaggcgc aggcaatgag caacggataa tcagaaccgg gtactcgccg ggcgacttca
    19141 cgggcgaaca ttgcaccgac aaccaaacca aatccccagt tgatgacaca agcgactgaa
    19201 ccgaagaaag taaccagcat gacgccctgt acgggcgtct ttgcggcgga ggcggcagta
    19261 cgcagcaaac ttttcaccgg agcggagctg gcaagggcat gaccggtaac gatgataagc
    19321 gccatctgca taccaaacgc cagcaagttc cagaaaccat caccccacat tttcaccatg
    19381 ctgatcggcg tttgtggtgt taaccaaagc gcgatcacga atgttagcaa tgtcagcaac
    19441 atggcaaaga tcagtggatc gggaagccac cggctgacaa aacgcgtcat aaaacgcgat
    19501 atgcgaccaa tcataaatca ccccgttgcg tattcagatc ggcggcaact tcaaaccgag
    19561 cttctgtttt ggcacgcacg gtggctaaat cacacccgtc ggcaatttcg gtgagccaca
    19621 ttttgccgtc aataaaacga aagacagcca gttcagtaac cagcatatgc accgcatgtt
    19681 gcgcagtgag tggcatggtg cagcggcgca aaatttttgc tgaaccatct ttggcgcaat
    19741 gttccatggc gatgatcact ttgcgtgacc cggtcaccag atccatcgcg ccacccatac
    19801 cgggcaccat tttcccaggc actacccagt tcgcgaggtt tgcttcttcg tctacttgca
    19861 aaccgccgag cacgcaggca tcaatatgac cgccgcggat tagcgcaaat gacatggcgc
    19921 tatcaaacat ggctgcgccg ggtaaaatgc cgcacggttg tccgccagcg ttcaccagat
    19981 ctggatgcgc tgtcgtgacc gggcctaaac cgaggaagcc gttttccgat tgcagagtga
    20041 tatgaatacc ctccggtaaa taattggcga ccattgtggg taaaccgatc cctaagttaa
    20101 cgatgtcacc atcacgaagc tcttgcgcca cacggcgcgc aatacgttgt ttcgcatcca
    20161 ttatttgctc tcctgtgaaa cgatgatgtg gtcgataacg gcaccagggg tgacaatatg
    20221 gtcaggttgc agctcgccgg tttcgaccag ttcatctggc tctaccagcg tgatatcagc
    20281 cgcaagggct atcagggggt taaagttgcg ggcgctaagt tgataggtaa ggttgccaag
    20341 tgtgtcgcaa cgatgagcgc gaattagcgc taggtcggcg cgcagcgggc gttcgagcag
    20401 ccaggtttta ccgtcgagtg tcagtgtctg tttgccttcc tctacgacgg tgccgacacc
    20461 agttggggtg agaaaaccac caagtccagc tccgccacag cgaatttgct cgattagcgt
    20521 accttgcggc accagaacga cgtccatctc accagatatc atgcgccgac ctgtttccgg
    20581 gttggtgccg atatgtgaag caatcacttt gcggactcga ccattgacga tgagcggacc
    20641 gatgccggta tcaacaaacg cggtatcatt ggctatcaat gtcaggtcgc gaacaccaga
    20701 ttccagtaat gcttcaacca ggcgggatgg agtgccaatc cccataaatc cgcccaccat
    20761 gatggtcatg ccgtcacgaa agaagccggt ggcgtcttgt aatgtcatca attttgtttt
    20821 cattttttat ccctcttgca tacaccgttg tgggtacagt cagggaatag caaggggagt
    20881 gccagaatga gattattgaa tgttgttatt taactaattg aatagaaagg aataaattag
    20941 atggggattc ataaggagaa tgagcggcta tgcagaaaat tgcgcactgt gcaaatttct
    21001 gcatagcaag ttttggcgtt atacatccgc cggatcgata ccgtattcct ggagtttata
    21061 catcaatgca cggcgactga tgcccagcat taacgcagtg cgggttcggt ttccttcttg
    21121 ttgttccagc acttccataa tgatgcgttt ttcgacgcgt ttaatttcct cttttaaatt
    21181 acgctcaccg acaggggctg tttttgcctc accagcatta cagactggct gacgaatctg
    21241 cggcggaaga tcctcagaga aaatgatcgg gcctgagttc atcacgacgg cgcgttcaat
    21301 gacgttggaa agctctcgaa tattacccgg ccatgaccag gcggtaagca gcgacattgc
    21361 catcggatcg atgtcgataa tatcgcgctg attctcacta ctgaattttt gcaaaaagtg
    21421 attagctaac agggaaatat cttcccggcg atcgcgcaga ggcggcagta ttaaatgaat
    21481 aacgttaagg cgataaaaga gatcttcacg gaaggtgcct tcttttacca ttgcctgcaa
    21541 gtcacggttg gtggcagcaa tgatgcggat atcaactttt atggtctgat gaccgccaat
    21601 ccgttcaaat tcccgttcct gcagaatgcg tagtaatttg gcttgcagta cgagcggcat
    21661 ttcgccaatt tcgtcgagga gcagagtacc ttcgttggca cgttcaaata atccctgacg
    21721 caaggtttgt gcaccagtaa atgcaccttt ttcatgacca aacagttcac tttcgagcaa
    21781 cgattccggc agcgccgcgc agttgacttt aatgaacggc ccctttgccc gccgcgaatt
    21841 gtagtgaatc gctctggcaa tcaactcttt cccggtgccg ctttcaccgc taatcaagac
    21901 gctggcctga gaaagggcaa ttttggcggt gtctttgcag atatccatca tcgccgggct
    21961 gttggtgaga atgtgccccc attgccagct ggtgctcagt gcctggtgca gatgacggat
    22021 ctcttttttc attgactgga gttgtaaagc gcgctgaacg attaaattca actcatcgag
    22081 atcaaacggt ttaataacat agtcgaaggc cccgcagcgt agcgcttcga cggcggtttc
    22141 cacttcagca taggccgtca tcagaataac gggtgtccgg gtctcattac tgcgcatctc
    22201 ctttagtgcc ttgatgccgt ccatctctgg catgcggata tccatcaaca ccacgtcagg
    22261 gtgaatatca gcaaacaggt gtaatgctgt gcgtccgttg ttcgcacaat gtgtttcgaa
    22321 accttgtagt gcaaaagcgg tgctcagcat acggcgaaca ttatcttcat catccacaat
    22381 aaggatgcga ttaatagcag tcatacagtc tgatttccct gcgggttgat cggtaaaata
    22441 agcgtgaagg ttgcgccgta gcccggcaaa ctggcgacgc gaatatcacc ctgatgggca
    22501 ttaatgatgc gttgacttaa cgccagacca agcccggttc ctgaggcttt ggtggtgaaa
    22561 aagggatcga agatcttttt ttgcagcgag agatcaatgc cacagccgtt gtcctctatc
    22621 gaaatggcct gttgtgagtc gctgtattgc caggttcgaa tgcgaatttt ccctcgtgca
    22681 ctgatagcct ggacggcatt gatcaggata ttcagtagta cctgtttgag cagttcacga
    22741 tcggcgttaa tcgggcttaa ttcattatcc agttcgctta tgaagtcgac ccgcgcttgt
    22801 acgccggcgg tttgtaccag taccagagtt tcttcaacca atgcattgag gctgacttgt
    22861 tgccattgac tgtggcgtgg gcgtgaaaat tcgagcaatt gctgaataac tttgttaatt
    22921 gaatcgattt ctttgagtac tacggacaga tattcctgat gtattgggtc acgggtttgt
    22981 tggcgcaaga tctgtacata accacgaata gctgttagcg gattacgtac ttcatgcgcc
    23041 acgccagcca tcagctcacc cagtgtggcg aggcgttctg cttgcgccat gcggcgctgg
    23101 gtttctttgc gggcagttaa atcagagaaa atcaccaaag cacctatcat ttcaccgtgc
    23161 gtgttatgaa tacgactggt cgtgacactg agttcaatgg tgcggtcacg gcctggaaaa
    23221 ctgatctcca gtgctacatg ttcggtgcca tgttccagcg tatccagtac gggactgtag
    23281 aactgagtat tgtcgaacaa catggagtaa ggctgcccta ccagttcatg acgttgatag
    23341 ccagtgatga cttctgctgc tgggttcatg gtggttacat caccctggcg gtcaatggca
    23401 atgacgccat cggcagcgtt ttcaataatc agatcgttaa gtgttcgcgt ttcacgcagt
    23461 gcctgggcga ggttgttaac actctgactg atttgcccca tttcaccggg caattgtggt
    23521 aatcgagtgg gaatattttg agccagagtc gagaggccat cggtgatgat atctatattg
    23581 gcgctcaggc gacgggagaa aaggacaatc aacagcaggc ttatcagcaa accggcggtg
    23641 agcacaatga taatcctcac atccattttc caggcctggc ggcgaatatc ttcggttaat
    23701 tcattggccc agatatagcc gaggatttca ccattacgct caatgggaat cattgaattc
    23761 aaaatatcgc cgcgcacctg tctgcctgaa taaaccaaag gggtatttgt acgcatgact
    23821 tcgcgaccag ggtgatctgc ggcaatggta acgcccacat tattctgata tagcgctgaa
    23881 ggcgcgtagg tgattatcgc atccagcgtt ttgttgtaat aaccagcgcc gatgccaggg
    23941 aaggcgtgag tgatattttc ggtaatgggg gcaagttctg catttaatgc gcggatacgc
    24001 tcctcacgcg gtaagtcgat gtagagatcg tagcgattgc ctagcgcctg attaagcagg
    24061 ttgaccacgg cagaaagttt tttctctttt tcagataaga ctgctgaacg tccttccgtt
    24121 tctacgatat aaccaatagt aagcgttggg acaatgacca tcaggattgc catcaggatc
    24181 atttgattac gtaagcggcg tggataaatc cacttcatat aatgcatgct gaaatccact
    24241 agtcttgtcc ggtatatgac gattatcaga ggttaaggtg atgatttctc ggcggtgtat
    24301 catattccag agaagagaga acattgcggt aacacgcttt taccgctacc ttaaccacac
    24361 tccatcggtc acctgaggcg gagcttcgcc cctttgaaat accttgcttc ttttcgtaca
    24421 accctgaaag cctcgcgcta catgttcaga gcattggcgt tagtgctctg gctgttgatt
    24481 gctttttcat ccgtttttta catcgttaat gcgttacatc agcgagaatc ggaaattcgt
    24541 caggaattta atctgagttc cgatcaggct cagcgcttta ttcaacgcac ctctgatgtg
    24601 atgaaagagc tgaagtacat agctgaaaat cgcttatcgg cagaaaacgg tgtgctttcc
    24661 ccgcgtggac gagaaacgca gacggatgtg cctgcgtttg aaccgctatt tgctgactct
    24721 gattgttccg caatgagtaa cacctggcga ggttctctgg agtcattggc gtggtttatg
    24781 cgctactggc gcgataattt ttctgcggct tacgatctca accgggtatt tttaatcggc
    24841 agcgataacc tctgcatggc caatttcggt ctgcgtgata tgccagtgga acgcgatacc
    24901 gcgttaaaag ctttgcatga acgcattaat aaatatcgaa atgcaccaca agatgatagt
    24961 ggcagtaacc tctactggat cagcgaaggt ccgcgccctg gcgtcgggta tttttacgcc
    25021 ttgacgccag tttatctggc gaaccggttg caggcgcttt tgggtgtcga gcagaccatc
    25081 cggatggaga actttttctt acctggtacg ttgccgatgg gggttaccat tcttgatgaa
    25141 aatggtcata ccctgatttc gcttaccgga ccagaaagca aaattaaggg cgatcctcgc
    25201 tggatgcagg aacgctcctg gtttggctat acggaagggt tccgggagct ggtgctgaag
    25261 aaaaatctgc caccctcatc gctaagcatc gtgtattcgg tgccggttga taaggtgctg
    25321 gaacgcattc gcatgttgat ccttaacgca attttgctga atgtgcttgc cggagctgca
    25381 ttgtttactc tcgcgcggat gtacgagcgg cgtattttca ttccggcgga aagcgacgcc
    25441 ctgcgactgg aagaacatga gcagtttaat cgcaagattg tcgcctccgc gccagtgggt
    25501 atctgcattt tgcgtaccgc tgatggcgtc aatattttaa gtaacgaact ggcgcatacc
    25561 tatctcaata tgcttacgca tgaggaccgc caacggctga cacaaattat ctgtgggcag
    25621 caggtcaatt ttgttgatgt cctgaccagc aacaatacca acctgcaaat cagcttcgtc
    25681 cattcgcgct atcgtaatga aaacgtggcc atttgtgtgc tggtggatgt ttcttcgcgc
    25741 gtgaagatgg aagagtcgtt gcaggagatg gcacaagcag cggaacaggc gagccagtca
    25801 aaatcgatgt tccttgccac cgtcagtcat gagctgcgaa cgccgctgta tggcattatc
    25861 ggtaacctgg atctgttgca aaccaaagag ttaccgaaag gcgtcgatcg tctggtgacg
    25921 gcaatgaaca actcttccag cctgttgttg aaaattatca gcgatattct cgacttctcg
    25981 aagattgaat cggaacagtt gaagatcgaa ccgcgtgagt tttcaccgcg tgaagtgatg
    26041 aaccacatca ccgccaacta tttaccgctg gtggtacgca agcagctagg cttgtactgc
    26101 tttattgaac cggatgtgcc agtggcctta aatggcgacc cgatgcgttt acagcaggtc
    26161 atctccaacc tgttgagtaa cgccataaaa ttcaccgata ccggctgtat agttctgcat
    26221 gttcgcgctg atggcgatta tctctctatc cgtgttcgcg ataccggcgt agggattccg
    26281 gcgaaagaag tggtgcgctt gtttgatccc ttcttccagg tcggaacggg cgtacagcgt
    26341 aacttccagg ggaccggtct gggtctggcg atttgtgaaa aactgatcag catgatggac
    26401 ggcgacatct cggtagattc agaaccggga atgggcagcc agtttaccgt gcgtattccg
    26461 ttgtacggcg ctcagtaccc gcagaaaaaa ggcgtggaag ggttgagtgg taaacgttgc
    26521 tggctggcgg tccgcaatgc gtcgctctgt cagttcctgg aaaccagttt gcagcgcagc
    26581 ggcatcgtcg ttacaacata cgaagggcag gaaccgactc ccgaagatgt gttaatcact
    26641 gacgaggtag tgagcaaaaa atggcagggt agagcggttg tgaccttctg tcgtcgccat
    26701 attggtattc cgctggagaa agcgccagga gagtgggtac acagtgtggc tgccccgcat
    26761 gagctaccgg cattgttggc gcgtatttat ttgatcgaga tggagagcga cgatcctgct
    26821 aacgctctgc cgttaacgga caaagcggtc agcgataatg acgatatgat gattctggtc
    26881 gtggatgatc atccgattaa ccggcgtctg ctggcagatc agttgggatc gttgggctat
    26941 caatgtaaaa ccgcgaatga tggcgtcgat gcgcttaatg tacttagcaa gaatcatatt
    27001 gatatcgtgc ttagcgacgt caacatgcca aatatggatg gttaccgctt gacgcaacgc
    27061 attcgtcagt tgggactgac gttgccggta atcggagtaa ctgctaatgc gttggctgaa
    27121 gagaagcagc ggtgtctgga gtccggtatg gacagctgcc tgtcgaagcc ggtaacgctg
    27181 gatgtgataa aacagacgct gacggtatat gccgagaggg tcaggaaatc gcgggaatcg
    27241 taggacggat aaggcgttta cgccgcatcc ggcaatccgt gcaaatgcca gataagacac
    27301 tgacgcgtct tatctggcct acaggtgatt agtctttatc tgccggactt aacgtcacag
    27361 aagagagata attcagcaga gcaatatcgt tctcgacacc cagcttcatc atcgcagatt
    27421 tcttctggct actgatggtt ttgatactgc ggttcagctt tttagcgatc tcggtcacca
    27481 ggaaaccttc cgcaaacagg cgtagaactt cactctcttt tggcgagaga cgcttgtcac
    27541 cgtaaccacc agcactgatt ttttccaaca ggcgagaaac gctttccggg gtaaatttct
    27601 tccctttctg cagcgcggcg agagctttcg gcagatcggt cggtgcacct tgtttcagca
    27661 cgatcccttc gatatccaga tccaataccg cactaagaat cgccgggttg ttgttcatag
    27721 tcagaacaat gatcgacagg cttgggaaat ggcgcttgat gtacttgatt aaggtaatgc
    27781 catcgccgta cttatcgcca ggcatggaga gatcggtaat caacacatgc gcatccagtt
    27841 tcggcaggtt gttgatcagt gctgtagagt cttcaaattc gccgacaaca ttcacccact
    27901 caatttgctc aagtgatttg cgaataccga acaagactat cggatggtca tcggcaataa
    27961 ttacgttcat attgttcatg tacttgcacc tatgtattaa ctatttgatt ttaaaaatat
    28021 attctatgct ttgtttagtc gttggggcac ctgtgggaca taagtacata gctttctgtt
    28081 tagtacttct acctgggact ggttattgtc tttcatccat gcaccgtaaa cattgtaaac
    28141 catctgggca ttcgcatgtc ccatctggtt ggctatgaag tttggatttg ctccggcagt
    28201 taaagcccag caagcataag tatgccgaga ctgatacgct tttctatggc gaatttttgc
    28261 tcgttttaat gcagattccc atgttctgtt gatggatgtt acggcataat gaactcctga
    28321 gttacctgtg cgatctaata tctgaggatt gaaaacaaag ctgcatgagt gagtgcttgt
    28381 ccggccatac tctcgcagct ttacttctat ctgatattgt ttaccaagcc tggttaatgt
    28441 cgcctgattt tttaatacgt caattgctgg ctgtatgagt tgtattaccc tgtttgttcc
    28501 tgcatctgtt ttgggtagtg taaattcctt tgtctgagtg taattccttt tgacggtcaa
    28561 ggtacccgct tccagatcga tatcctccca tgccagccca cacagttcgc catgccgcat
    28621 cccggtatat acagccagcg accagaaatt tttagtttgc tggtggtggc acgcatcaat
    28681 cagtctggca aactcatctt ttgttagtgg atcgggaggt gttttcgctc ttttcagtga
    28741 tggaagctca ctgaaagggt ttttgcctat atacccattg tttgccgcaa aacggaacat
    28801 cccggagata attgtcatat agctatttac agtaggtaca ctacgacctt taataggctt
    28861 cctctgattt tttcctggca actgatatcc cgttaataat tctttacgaa aaatgagaac
    28921 atcttcctgt gtaattgatg atgccagttt gttccctcca aggcagtcaa ggcaatttcg
    28981 gatagctgat ttgtatctga ccatggcatt tgctgttatt tccattttct taagctcaag
    29041 ccatttttcc gaaagtgcct taatggttat ctcttttttt cccagaccaa aatgtttcag
    29101 gttaggggaa ttagggaact gcgcggcgta gtcgaagctc cccattctta ttgcaaaaca
    29161 aacggaagtg cgaagttcac ctgcgatctt ccggtttttg gcggtgtcag gaacaccgag
    29221 gttttctctg acacgtttgc cgttatagtg aaaccatatg cggagtgatc cgccatggtt
    29281 ttcaacgcct gtcgggtatg atgcgttact cattaaacct cccagacgtc caggagcatt
    29341 aacaggttaa ccggaacttg catttttggc acctggttgt ttctggtttt cgatccagcg
    29401 cataatttcc tcaatgttgt atacacattc actgaaaggc ccgggctctc cttctgctgc
    29461 gtaatgacgg tattctttcc ccagtaacca ggattttttt cttgctctgg caatggttcc
    29521 tgatttcagt ccagttgcag caataagggt tctttctgtg caccatttgc tgggcgttat
    29581 ctgatagatg attgtttcca ttcctgactc acactatctt caggccacgg cagtggcacc
    29641 acacgtcaaa cattcgcttt acaacttcac ggcagtagaa gccgtgagca tctctggtta
    29701 aatcgtatcg gtacccgtat ctgagtcgta tccatatttc aaactcgcga ttcacgactt
    29761 attctcctgc tgtctttatg ccagttcatt cggttgcgct ggggccctgg ccttccgtgg
    29821 cctgtatttg cggcgttttc tggcttcaat gcgtatcttt gtcaccctga ctggctttgg
    29881 ttcgtgccgg aacactttgt ttctgatatt acgccgtgta ttcatcatcc agataaaacg
    29941 ctgggtatgg tcgcatccgt catcaacggt aattactgtg cggaccagtt catcgttaat
    30001 atcaaccatt tcacccattc actgagcctt catacgcgaa tttcgtggct tccatcagtt
    30061 gcgtccatac ccattcaaga aattgtgcgt tttctcctga gcggtcatat tcatcgacgc
    30121 tgcggggctt gatggtcgcc agtagtggca caatccggta cttcatctgt tcggttaact
    30181 tgtccggtac aagtcgccag ccgttatatt tcgatggaac atggtggtat tcagccagga
    30241 agcgtcgtat atcgtttttc atttcctcgc atgagttctg atcatgaagt gcagcggaaa
    30301 gccagcgtcc gatatattca gggattcgca catcaagtac tttttctgcc gtcgtcagca
    30361 ttgcctgtcg gcaggcgttc cagccgttga catagtgacg tgttgcatcc catgaaacct
    30421 gcacatcagg gtaagcatcc tgttctgttc tcactggcgg tatctgagga acagtgaata
    30481 gtggactgac gatatattta tccagagagc ctccccgctc ttctgcaaga ctttcccacg
    30541 atgtcattgt ggggaccctg taacctgctt ttttgtgcgt atacatccat gccactctgc
    30601 cgttatcatc tgcggcggca atgcgctgcc aggcaggata aagcggggtg tatgtttctc
    30661 tgtcattacg ggcgtcaggc atggcatcaa tactgcaaac ttcccccgta aggttattca
    30721 gccaggcctt cgggctaatc tctgacgtta cgatttcatc cagttttttc tccagatttt
    30781 ctatgtgact gattagcgca aggataactg gcggagtggc aagggagtga aatgcttcca
    30841 gatcacatcc cttgtcgcca tatacggtat tttgtgcggc gtcacgcagt gctttcagat
    30901 cagcttcttt catcgttcag actcccttgt tgctgttttg taggcgcgca ggatgtcgcg
    30961 tgtttttcct gataatgacg acttaaccag gaaaaagccg ctccggcttt ctgtgatgtc
    31021 cggcgtgcac agaagtaccg catcaacgac acgattatgt tttcgcaatg tcaggcggga
    31081 actggtgata atcagttttg cctgttcgcc gtaatcggtg aatgaaataa tcatctgatg
    31141 gcgatctccg ttatgaaagt gttcctgctg gttcaatgct gataatttct gctgcaataa
    31201 gcatacctgc cagccatgct tcgccgagca tgtcctcatc cgtacatgca agatcaccga
    31261 tctgtattgt ggccataatg ttagtttcac cggtatattc atcctgtgct tcttcatacg
    31321 gcaggtcttc ataaaggttt tcaattgcgc aggttattac atccagtccc gtcagattgc
    31381 cgacaacgtc cacttcaaat gtttcgcggt actcccattg cccgagtgtc agacgaaccg
    31441 tctgtcttgc catgcgcccg cataatgtca tgttcgggtc gtagttcatg attgccgcag
    31501 tagtgctatt catgttctgc ctccggtgtg taaatagctt tgtcgtgttt aaattcaccg
    31561 ttccagtttt tcttcatagg gagttcaccg ttcatgtaaa actggtataa acggtggcat
    31621 cctttttcca gcaatacggg ggtgaatttc gtgaatgttg ctttcccgtg tggagtgatc
    31681 tgtactgctt cttcggtcag gtatctgtca cgggcataag aggccgtgcg ccagcggggt
    31741 gttttctccg gatcgcgttg ttcgttgaat acccattttc ggccagacag ccaccacatt
    31801 atctggctgg tatttacgcc gttcagcgca cggcagaact ggggaacagt tatgccttta
    31861 gagaaatgtt tttccagact gtctacggtt gcagagagcg ttttattttc cagggccata
    31921 gcttcagccc gttcttcggc ctcaataacc atcaacgcga gttccttgcg agtgagtacg
    31981 cgctgaacat cgatgttttc ccgctgagtg aagtagaact ccacaaggtc ttcgtgatag
    32041 ttccacgcct gatccgtttc cagcaatttt gcgtggttgg ctgcgccgcg ttcggtccag
    32101 aggatgaggg atcgaacatt gctggcaatt ttcacagagt agctttgaga tactctgtgc
    32161 ttaagttcac gtagttcatc gccttccagc aaaaaataat gctttccaac aacgaatcgt
    32221 tcttcgtttc gggaatgatt ctggcgaata cggattggtg ttgctccata acctgccgcc
    32281 agttgttcag tggtcacaac gcgttgaccg cgatattcga taatttgcaa atcacgggct
    32341 gccaccggca caagttcagt tgattttttc atcattacac ctcacgaaaa gctgattagt
    32401 ggaacactct ggtttccggt tctgagttgt cagtggcttc actgataagc ccatccagaa
    32461 tcatgtgcat cgctctgtaa ccttccattg tgatgccggt tttttcgccg tcgcccagag
    32521 cgccggtgaa tgctttgtac ataagaatgc ttttgagcag ccctgattcc gctccgtaag
    32581 tttctatgag tgcccattcc atatagttct gcatagcgag acgtaatggt ttcaggtaga
    32641 ttgtcatgcc accgttgtgg cctctgtaca tggtgccctt gtctgtaccg ccattctcgt
    32701 taagcacttc ggtccagccg tttctggtga gctgttcaga gaagaacatg gcggtataaa
    32761 gccagcgcca gtacatgatt gtcttttccg gcgcgagctc acctgtcatg cctttttctt
    32821 ctgcttcact gatgcaacac actatttcca gaccatgcca gatcggctcg tcaaaacagc
    32881 cttcatcgag catttccagt gctttgttga tatcaagcag tccgtaaggt gtgtggatac
    32941 cttcagcggt aagaaaaaat aactgttcag ttgcagtcat ggtttgctct cttcttaaat
    33001 ggtctttgcg cgacaagccg gcaatactga tcaatccagg cgttgtattt ttccatccag
    33061 gcatcaaact gttttctgcg tgcaaggatg cgcaacaggc gatgaaaaca acgcccgtga
    33121 gctctgtagt attcgggcgt aatttctccc ggatgaaaga cttctccata ttccggatgc
    33181 atcaggcact ggtcaggcag acgtttttca aaacctgaac tttcgaatac cttcgttgtc
    33241 ataaaatgag cgagatgtat gatggccgtt tttcgcgtga agcatttctt aacttgtccg
    33301 tggcggcaga ctctgtaaac agggccgcaa tcgagttcgt atttatgaaa agcacggtcg
    33361 attccggcat tatttattgc actgtattcc gcatcactga agaaaggtgg aaatacgatg
    33421 tatccttcca ttgtcagcgc cagaacgcgc ggatcggttt ctgtcagcca ttcgttttct
    33481 gtgaattctt cagaattaat ttcaatgatt tcaggttctt ctttcatggt tactccgtgt
    33541 tcatccggtg ttaattactt ttattcactc tgagcgcagc cagaagatgt ggctcagcaa
    33601 ttcgtttgtt gcgatatatc tgtgatacag cctggccttt tcaggttgta aaaatccccg
    33661 acaataaagc tgtaaattaa ttactgttct taaataatgt tattcgtttt cagtccggca
    33721 cagatgcttt tctgctttgt ctgctgtaca ggataatgag cggaaaagac aattcatcat
    33781 gtttagcgca tctggtgaac caatatcacc gctgtcaata atcacctgta acaaagacag
    33841 gctttctgtt atctcgcagt ggacattatc gagtacttca acgcaattat tcatttttca
    33901 ttatctccgt tattccgggg gaggagctat gctgttaatg attaacgcat ctggtaatat
    33961 gcccctcttt aatgtaatcg atgcattcat tcaaaagttc atcaatgatt aactttcctg
    34021 attcagtcag atactcacca tgttggttga taccgacagc atcctggtat gcagtgcgta
    34081 ttgctgtttc accatctttc tgcccaaact caccacgggt aacgccttca aatcgtaata
    34141 gcaactggct tatgaactgt tctgttattt caatagttgt taactctcca tcatgacgct
    34201 ttacagtgag gagattactt cctgtttttc gtttcatccg ccgcagtgct gcaaccgtta
    34261 tgcggcgacg atatgcagaa atgtattcat ctttcatttg ttatttcccg tatgcctttt
    34321 tgagaaacaa tactgcaact gaccagtatc cggcatcagc cattaataaa gcggttttat
    34381 aggcatgttt gtctttcatg tgtcatcccc attttaaaat agtgagaact cccgaccgca
    34441 gaccgtcatg tttttatgta tgattgtctg tttaatttcc tttattcgtt ctcagagcat
    34501 caacatactc gtaagccttt tcacaggttt tattcatgga acgaatcata caaagtaaga
    34561 aatcatctgt ctctccgtta tcaccagagt ttttaaatat aaactcaagc attgacgtgt
    34621 tttcttttat ttctgctgcc acttcctcaa gcataatcag gggagttttc atgttctttg
    34681 ttccttaaat gcatcgcatg cgcttctggc gtattgttgt gccagtaaaa atatgtcatc
    34741 cgaaagttca tcacattctt catcaccgga agccgaaatg attaaccccg cttcaagcag
    34801 tactgcaatg tgatgaaaag ctgtttccgg ttcgttggtg aggcctttga acattttcat
    34861 cttacgattc ctttgtcgtg tcagaatgga aacttgtaag taaccacatc ggatcgcagt
    34921 caagtatatt tgccagagga atcacctggc ttgccagagg ttcagccttg ccgttctccc
    34981 actggatgat ggtttcttca tcaactccaa gcagcgtggc gagttcagca gtggatagac
    35041 cgcagctttc gcgttgggtg cggatgccat cacggcaatc ttttttgttt ctcggaggta
    35101 acagaatatg ttttagttgt tggatttttt cgattccaac ctcaaggagt acgtgacggt
    35161 ttctttccga tacagactcc agactttcat gaagcagcga ttctacttgt tcgataagca
    35221 tcaattgtga agtagacata aaaaaacctc gtaaccaata tttcgaaatc taggttacga
    35281 gtgagtaatg taggtgtcaa gagcaattaa tgttttttgt tgaattaaat ttgcatacgc
    35341 atctcaacga caacaccaat aatttcacaa tccccattga tggggatcag tggatagcgt
    35401 gggtttagtg gctttaggaa acattgccca gcgtcttcta tgtactgttt aagggttgcc
    35461 tctccggagt ttttgagcct ggcgacgaca tattttcctg aaaaaacttc tttgtcaggg
    35521 ttaacaagaa tgttcatgcc ttcaaaaata gagaactctc cattctgtga tgtcattgag
    35581 tcccctttca cttccagcca gaagccgtga tcgcccgcat atatatcgct accaatccat
    35641 tcttctggat aagctatgta atcttcgcag ttccaggggc cagcactcac ccatgttagt
    35701 ttcggatatc tataacggtt tggagaaact ggcttaggat ttcggacgtt ttgaatgcta
    35761 tctgtaggtg atggggcgct gccgtcaaga agccatttag gagagcattt caatgcatcc
    35821 gcgattctaa ataggtttga agatcttgtg tcttgtgagt ctcctaactc tattttgctg
    35881 attgttacac gcgagacacc ggtcaatttt gccaggtcat cttgtgacat tcctagctgt
    35941 actctgcgca ttaacagtct gccaccaaag ctatcgtgat ccatcttctc ccccttcttg
    36001 tcgaaataat cgtttcgaaa caaatatagc aaaaaatctt gaaatctagg tttcggaaaa
    36061 ttaagattaa tcgaaacaaa cgtgtcgagg tggatatgga tttgtatgaa attctaaaaa
    36121 acatgtttgg atcgaatgtt gaaataggtc gttattttcc ccgaagggga agggctagaa
    36181 ccggacaggc tgtaggtaag tggaaaaccc gaggtgtgcc ggaagacgtg gcgatccttt
    36241 gtcatctgga tcccaaaatt ccttatcagc atccgtcgct catgaatgcc agtcatgaat
    36301 cgtgatgttc tgccggaggc tgtatgtccc ctgattacgt tcagatggag atgcctgcca
    36361 ggtacagcca ggcagattca gagtggatta agcggcagtt acagagcctg ccttcctcac
    36421 tgagacagaa ggtcgccctg aaatacgcgg aggtttacga agttactttt gattccgagc
    36481 ctgtttcatt ccgtaaggag aacagagcga ggcatgaagc caatgtacgg ctgcgcagat
    36541 tcgttgaaac acacgggcgt gcattacagg ggtatacgac tcagccgccc ctggcaggaa
    36601 cgcaacagcg tacctgaatg gtgttggtct taaaggtgac cgacgggctg agaccccact
    36661 tctcaggatg cacttatgta ctaccaagct agta
//
LOCUS       DAZKGF010000025        35442 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZKGF010000025 DAZKGF010000000
VERSION     DAZKGF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.025"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..439)
                     /locus_tag="ACHOQV_001261"
     CDS             complement(<1..439)
                     /locus_tag="ACHOQV_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP9629659.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGV"
     gene            complement(642..1043)
                     /gene="nikR"
                     /locus_tag="ACHOQV_001262"
     CDS             complement(642..1043)
                     /gene="nikR"
                     /locus_tag="ACHOQV_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFP9629660.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVSTLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1048..1854)
                     /gene="nikE"
                     /locus_tag="ACHOQV_001263"
     CDS             complement(1048..1854)
                     /gene="nikE"
                     /locus_tag="ACHOQV_001263"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFP9629661.1"
                     /translation="MTLLNVCDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1851..2615)
                     /gene="nikD"
                     /locus_tag="ACHOQV_001264"
     CDS             complement(1851..2615)
                     /gene="nikD"
                     /locus_tag="ACHOQV_001264"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFP9629662.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENTARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2615..3448)
                     /gene="nikC"
                     /locus_tag="ACHOQV_001265"
     CDS             complement(2615..3448)
                     /gene="nikC"
                     /locus_tag="ACHOQV_001265"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFP9629663.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3445..4389)
                     /gene="nikB"
                     /locus_tag="ACHOQV_001266"
     CDS             complement(3445..4389)
                     /gene="nikB"
                     /locus_tag="ACHOQV_001266"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFP9629664.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4389..5963)
                     /gene="nikA"
                     /locus_tag="ACHOQV_001267"
     CDS             complement(4389..5963)
                     /gene="nikA"
                     /locus_tag="ACHOQV_001267"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9629665.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6074..6661)
                     /gene="acpT"
                     /locus_tag="ACHOQV_001268"
     CDS             complement(6074..6661)
                     /gene="acpT"
                     /locus_tag="ACHOQV_001268"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFP9629666.1"
                     /translation="MYRIVLGKVSTLSAAPLPPALREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLDNLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6716..7765)
                     /locus_tag="ACHOQV_001269"
     CDS             complement(6716..7765)
                     /locus_tag="ACHOQV_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9629667.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7897..9114
                     /locus_tag="ACHOQV_001270"
     CDS             7897..9114
                     /locus_tag="ACHOQV_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9629668.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9118..9675)
                     /gene="dcrB"
                     /locus_tag="ACHOQV_001271"
     CDS             complement(9118..9675)
                     /gene="dcrB"
                     /locus_tag="ACHOQV_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFP9629669.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(9748..10413)
                     /locus_tag="ACHOQV_001272"
     CDS             complement(9748..10413)
                     /locus_tag="ACHOQV_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFP9629670.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10635..10880
                     /gene="tusA"
                     /locus_tag="ACHOQV_001273"
     CDS             10635..10880
                     /gene="tusA"
                     /locus_tag="ACHOQV_001273"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFP9629671.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDELPYRYLIRKGG"
     gene            complement(10982..13180)
                     /gene="zntA"
                     /locus_tag="ACHOQV_001274"
     CDS             complement(10982..13180)
                     /gene="zntA"
                     /locus_tag="ACHOQV_001274"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFP9629672.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVMLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPAAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13254..13880)
                     /locus_tag="ACHOQV_001275"
     CDS             complement(13254..13880)
                     /locus_tag="ACHOQV_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFP9629673.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14021..14380
                     /locus_tag="ACHOQV_001276"
     CDS             14021..14380
                     /locus_tag="ACHOQV_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFP9629674.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDI
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14383..14652)
                     /locus_tag="ACHOQV_001277"
     CDS             complement(14383..14652)
                     /locus_tag="ACHOQV_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFP9629675.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14642..15238)
                     /gene="rsmD"
                     /locus_tag="ACHOQV_001278"
     CDS             complement(14642..15238)
                     /gene="rsmD"
                     /locus_tag="ACHOQV_001278"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFP9629676.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15388..16884
                     /gene="ftsY"
                     /locus_tag="ACHOQV_001279"
     CDS             15388..16884
                     /gene="ftsY"
                     /locus_tag="ACHOQV_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP9629677.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPELVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16887..17555
                     /gene="ftsE"
                     /locus_tag="ACHOQV_001280"
     CDS             16887..17555
                     /gene="ftsE"
                     /locus_tag="ACHOQV_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP9629678.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17548..18606
                     /gene="ftsX"
                     /locus_tag="ACHOQV_001281"
     CDS             17548..18606
                     /gene="ftsX"
                     /locus_tag="ACHOQV_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP9629679.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18851..19705
                     /gene="rpoH"
                     /locus_tag="ACHOQV_001282"
     CDS             18851..19705
                     /gene="rpoH"
                     /locus_tag="ACHOQV_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFP9629680.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19977..21080
                     /gene="livJ"
                     /locus_tag="ACHOQV_001283"
     CDS             19977..21080
                     /gene="livJ"
                     /locus_tag="ACHOQV_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFP9629681.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQVGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21268..21651)
                     /gene="panM"
                     /locus_tag="ACHOQV_001284"
     CDS             complement(21268..21651)
                     /gene="panM"
                     /locus_tag="ACHOQV_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFP9629682.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            22075..23184
                     /gene="livK"
                     /locus_tag="ACHOQV_001285"
     CDS             22075..23184
                     /gene="livK"
                     /locus_tag="ACHOQV_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFP9629683.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANKGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            23232..24158
                     /gene="livH"
                     /locus_tag="ACHOQV_001286"
     CDS             23232..24158
                     /gene="livH"
                     /locus_tag="ACHOQV_001286"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFP9629684.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            24155..25432
                     /gene="livM"
                     /locus_tag="ACHOQV_001287"
     CDS             24155..25432
                     /gene="livM"
                     /locus_tag="ACHOQV_001287"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFP9629685.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGSFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            25429..26196
                     /gene="livG"
                     /locus_tag="ACHOQV_001288"
     CDS             25429..26196
                     /gene="livG"
                     /locus_tag="ACHOQV_001288"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFP9629686.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            26198..26911
                     /gene="livF"
                     /locus_tag="ACHOQV_001289"
     CDS             26198..26911
                     /gene="livF"
                     /locus_tag="ACHOQV_001289"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFP9629687.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            26999..27220
                     /locus_tag="ACHOQV_001290"
     CDS             26999..27220
                     /locus_tag="ACHOQV_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9629688.1"
                     /translation="MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEF
                     ERYQKAKLDAEFDAIMAIHGSEIKELADK"
     gene            27217..27594
                     /locus_tag="ACHOQV_001291"
     CDS             27217..27594
                     /locus_tag="ACHOQV_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP9629689.1"
                     /translation="MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNK
                     YEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALFITLLFLKRNGIILAANPEFVEL
                     TVDVAAGKVSLEDIVTRLRQRGT"
     gene            27803..29119
                     /gene="ugpB"
                     /locus_tag="ACHOQV_001292"
     CDS             27803..29119
                     /gene="ugpB"
                     /locus_tag="ACHOQV_001292"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFP9629690.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEQSTKS"
     gene            29295..30182
                     /gene="ugpA"
                     /locus_tag="ACHOQV_001293"
     CDS             29295..30182
                     /gene="ugpA"
                     /locus_tag="ACHOQV_001293"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFP9629691.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            30179..31024
                     /gene="ugpE"
                     /locus_tag="ACHOQV_001294"
     CDS             30179..31024
                     /gene="ugpE"
                     /locus_tag="ACHOQV_001294"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFP9629692.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            31026..32096
                     /gene="ugpC"
                     /locus_tag="ACHOQV_001295"
     CDS             31026..32096
                     /gene="ugpC"
                     /locus_tag="ACHOQV_001295"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFP9629693.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLKGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHITLRSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVLRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            32093..32836
                     /gene="ugpQ"
                     /locus_tag="ACHOQV_001296"
     CDS             32093..32836
                     /gene="ugpQ"
                     /locus_tag="ACHOQV_001296"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9629694.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(32823..33263)
                     /locus_tag="ACHOQV_001297"
     CDS             complement(32823..33263)
                     /locus_tag="ACHOQV_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFP9629695.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            33383..35128
                     /gene="ggt"
                     /locus_tag="ACHOQV_001298"
     CDS             33383..35128
                     /gene="ggt"
                     /locus_tag="ACHOQV_001298"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFP9629696.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGAPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGEYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
     gene            complement(35166..>35442)
                     /locus_tag="ACHOQV_001299"
     CDS             complement(35166..>35442)
                     /locus_tag="ACHOQV_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFP9629697.1"
                     /translation="YHDAFAKANHYLDDADLPVVITLHGRFIQGWYFCFESQEFLESG
                     DEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         8239 a         9168 c         9936 g         8099 t
ORIGIN      
        1 ccacgccgcc gcgcaccagc cagagtgact cgctgcggct gtaaccgtcc tcaccgggaa
       61 acaggtgctc aaaatacagg cttctgccgc cgttatcact gagtatcagt gtgttatcgc
      121 gcagctgtaa gcggatatcc gcaggcattt tccagccggg gccgaggctc cccaccggcg
      181 cgggcgtttt tgtccggtaa ctgctgtagg tgcgggagag aatgaacggc agcgggccgg
      241 gcagggcaag gtcggtttcg ccgggaagga ccttcgcacc gagcagcgga ttgaccggat
      301 gaccggacgt caccccgccg gggcacaccg aacaggccac accggtgggg gcaccaatac
      361 gtaccccggc agaaccctga acaatgctac cgccatactg cgtcatgtcg ccctgacgcg
      421 ccgccggttt tccgctcatc gtaaaatcct tttctattat tttaatgaca gtatcttcat
      481 acgaaaaagc gaagaaaaac ttgtggtcac aatgaattga agatttttag gaatatattt
      541 agaacgttac aaaagagaag gttcggatag ggtatttaga gaggaatcag gtgtgctagt
      601 ggccctttgt tgatcgttca ccaaaggacc caataatagg cttaatcttc cttcggcaag
      661 cactgcaaat gcccgtgccg cacgccgcgc tgggcgataa cgtcatcggc aaaatgctgc
      721 acgtcgccca tatcgccttt cagcacggcg atttccagac agtcgtcgtg attgatgtgg
      781 acatgtagcg tcgagacgga gagatcgtgg tgatggtgct gggtggagac aatacggctg
      841 gctaagtcgc gtttttcgtg ttcatacaca taggagagca ccgcgaaacc ttgcgtgccg
      901 tgctgctggg tagcttcctg cgccagggcg ctacgcagaa tatcgcggat agcttcggaa
      961 cggttgttat agccacgacg ctggctcagg ctgtccagcg tctccagtaa atcgtcatca
     1021 agcgtgatgg taactcgttg catagactta aaccttttct gtggtgcgac ggcgcacggg
     1081 gaatgcgggt aataccgcgt tttgtagcac acgtccggcg tcagaggaaa aggttaattt
     1141 atctcccacc acctgggttt cgacgatttg tccgttgtcc atcaccatca cccgctgaca
     1201 gaagcgttcc accaggcgta agtcgtgggt gatgaacagg caggcggtgc caaactgttg
     1261 ttgcagcttt ttcagcaggc gaatgacgcc cgcctgtaac acgagatcaa ggttagaaac
     1321 ggcttcatcc agaatcagta gtttcggttc gaccgccagc gcgcgagcca ggcagacgcg
     1381 ctggagctga ccgccgctta actgcggtgg gcgtttgtct agaacgctgt catcgagatc
     1441 gaccgccttc agcatttcgc tggcacgcgc cagttgttcg gcttttttca gcgatagcag
     1501 gtggcgcatc ggttcacgca ggatctcgcg cacggttttt cgcggattca cggcgctgat
     1561 ggagtcctga aataccatct gaatatcgcg gcggaacgct ttacgctggg cgcgattgag
     1621 ctttgccaac ggttcgccgc gccagctaat attcccttgt gagggagact ctaagcctac
     1681 cagcagccgc gcgagagtgc tttttccgca gccgctgcgc cccagcagag cgacggtttc
     1741 gccgcttttc agggtcaggg aaacgttatt cagtaccgcc tgatgctgat gttttccgct
     1801 aaatccaccg tgcgcatagt gatgtgaaag gtcgcagacg ttaagtaaag tcatgatgcc
     1861 agctccatac cgtagagggc gagatgagcg gaaaccaggc tgcgcgtcac cgtatgtttg
     1921 ggggcgttaa acagcgtttc tacatcgccc tgttcgacaa ttttaccgtg tgacatcacc
     1981 gccacgtcat ccgccagacg cgccacaacg cccatatcat gggtgaccag cagcattccc
     2041 ggtgcttgtt tttgcataat gctttccagc agatcgagga tgcgcgcctg tgctaccacg
     2101 tcgaggtcgg tggtcggttc atcggcgatg ataaacggcg attcacacaa caccgccatc
     2161 gcaatcatca tgcgctgcaa catgccgccg ctcatctcga acgggtacag cttcaacacg
     2221 cgcgcggtgt tttccaaccc caccgcttct atggcagcgg taagcgtagc gtcatcggcg
     2281 ggtttcccca gcgccaggca ggtttcacgc gcgtgggtgt gcatggtgtg tagcggatta
     2341 aaggcgctgc gcgggttctg catgatggtg gcaattttga tgccgcgcag ggcacaggga
     2401 gaaaccggtt tgccatcggc taaaatttcc cccgccgtct ggcgaacgcc tgcgggcaga
     2461 atgcccagcg tcgcggcgca ggtcagtgat ttcccgctgc cgctaccgcc gactaacgcc
     2521 agcacacgcc cgcgtttaag ggttaacgat acgccgtgca ccagcggttg tgcggcctgt
     2581 agcgcgatat tacgtagttc aatctgttgc ggcattagtg tgcgtgctcc gtcaccagat
     2641 gaggatccag atgatcgcgc agtgcgtcac ccaccaggtt aaaggccatc acgctgataa
     2701 acagcgccag ccccggccag aacatttgca gcggctgggt ccagatatac tgacgcgcgt
     2761 cgttaatcat cacgccccat tcggcggtcg gcgcggtcac gccgagaccg aggaaggaca
     2821 tccccgcgac gtgtagcatc atatgaccga tatccagcgt cgccagcacc agcagcgagg
     2881 ggatcaccgc acctgccaga tgatcaataa acacccgcac atggcccgcg ccagaaagcc
     2941 gtgatgccag cacaaactcg cgctggcgca gtgaaatcac caggctacgc accatgcgtg
     3001 cataccacgc ccagtgcgac agggcgatgg ctataatcac attggtcaac ccggtgccga
     3061 gcacgccgac cataaagaac gacagaatcg aggttgggaa ggtcataaac atatcagcga
     3121 cgcgcatggt ggcctgatca acgcgcccgc caatcagccc ggcgctgccg ccaataatca
     3181 gccctaatgt cagcaccaac agcaggcagg ccattaccga gccgagcgac acgcgggtcg
     3241 ccgccatcag ccgcgagaaa atatcgcgcc ctaaatgatc ggtacccagc cagtgctgcg
     3301 catccggtga aagcaggcgc gacggcagat caatcgcctg ggggtcatac ggcagccacc
     3361 actggctggt gagcgcaatc agcgccagca gggcgataat gatcagcgcc aggcgtaccg
     3421 accagcggga agagaggaaa aagttcacgc gtgcgctcct tcatgacgac gaatgcgcgg
     3481 gtccagcgcg gcgttgagta aatcaacaat caaattacag accacaaaaa ccaccaccat
     3541 catcagcgta aagcactgga tcaccggata gtcacggtta aaaatcgccg acaccgcata
     3601 gcgcccgacg cccggccagg caaagatgtt ttcgataatc atcgtcccgc caatcagttc
     3661 gccaatgtgc atccccacgg cggtgatcat cggcagcgag gcattgcgca gaatatggcg
     3721 gcgttcggtc tgtttgtcgt tcaggccgcg tagccgagcc caggtgacgt gacgctgacc
     3781 ggcgacgtcc agcatactgg cgcgcagcag ccgcgcgtta atcgccagcg acataaaggc
     3841 aatggaaacc gcaggcaaaa tgatgtgctg ccagccgccg tagcccatcg cgggtagcca
     3901 ttgcagatat accgaaaacg ccatcaccag caaaaacgcc agccagaagt tgggcatcga
     3961 cacgccgaga aacgcgatga aacgtacggc gaaatccggc agacgatcgc gatggcgcgc
     4021 cgcccagata ccgagcggta cggaagtgag cagaattaac accagcgcgg cacctgcaag
     4081 ttccagcgtg gcgggcagga agttcagcat atcgtccagc accgggcgtt ggctggcgaa
     4141 tgagatacca aagtcaagat gcagcgcctt ccacaaccag gtaccgtact ggacgtacag
     4201 cggctgatcc agccccagca tggtgcgggt agaggccagc atctccggcg tcggcggcag
     4261 gttagataaa cgcagataat cgagcgccgg gtcgccggta ccgaggcgca gcatcagaaa
     4321 aatgatcacc gaggcggcga gcaccatcgg gatcagcagc agaaagcggc gtaatacgta
     4381 acgcaacatt aaggtttcac cggtttaatc tgttcgaacg gaatttcggt ggcgatcggc
     4441 gcgtagggga tgttacccag ctccggtttt gataccacca tcattgagat gtaactgata
     4501 ggcagataaa ccgcctcgtc atgcagacgg gtcagaatgt cgcgatacag cgcctgacgt
     4561 tgcgtttcgt catgggtcgc cagcacttcg ccgatctctt tatcaatcag cggcttgtcg
     4621 gctaaacctt gctgtgcctg gaagtcagcg tgcgacggta cgcgcattga actcaggaag
     4681 gcgtgtggat cgtatggcgc gccccaggtg cggtggaaaa tcatgccaaa acgaccgtcg
     4741 cgctgacgag catagatact gctctcttct tcgccaatca gcgagacatc tgcgccaatc
     4801 tggcgcatat cagcctgaat gatttccgcc atcgatttgc ttaacgcatc ggtgccgatg
     4861 aacgacagtt caatgcgcag cggctgacca tttttctcgc ggatgtcttt gcctgcaggc
     4921 agcgtccaac cggctttttc cagcaacgct ttcgctttct gcggatcgta ctggcgcggt
     4981 ttcaggccga ggttggcgta gggcacagaa ggggcaaaca gggtgtcggc gacctgctgg
     5041 gtaccataca acgcgttatc aatcagcgat tttttgttca ccgcgtaatt aagagcttca
     5101 cgtactgcca gctcgttggt gggggctttg gcggtattga gcgccagcat cacggtttcg
     5161 atcggctgtg acagttgagt gtggtaagcc gggttctggc taaagcgggc gaaggtatcg
     5221 agcggtaata acccttcgtt tccgtacagc aggtcgatat cgccagtttc aaacgccacc
     5281 gcacgggtag tcgggtccgg gatcacgtta aaggtgatct ttttaatcgc tggcttttcg
     5341 ccccagtagt tttcgttacg gacgaagaca tcgtactgat tcagtttcga ttcctgtaaa
     5401 acccacggcc cggtgccaat cggcgcttta attccgttca tggtttcgtg gtttttaaac
     5461 tgcgagggtg cgataaaacg gaaaggacgg ggcagggcca gttcttgcag gaaaggatag
     5521 taggcgcttt tcagggtgat ttgcagctcg tttttactga gtgctttgac atcaacaatc
     5581 tggtttgcca gctccagcca ggcgtgacgt tgacggttat cgagcactgc gcggaagttt
     5641 tctgccgccg cctctgcatc aaacggttcg ccgttggaga atttcacgtc atcacgcagg
     5701 gtgaaggtcc aggttttacc atcttctgaa tgagtccagc tttttgccag ccacgggatc
     5761 accgaaccgt ctgcctgata tttcaccaat ggttcataaa ccatgctctg ggcgaacatc
     5821 tggttaggcg tgtaaaggtg cgggtttagt ggcccgacat tcaccggcca ggcggtggtg
     5881 atttcatctg gtgcagcggc atggacgata aaagacgcac aagccagcag cgcaaataga
     5941 gtgcggcgga gtgtggagag catggtaacc ccaatggatt aaaatagatg gcggaaataa
     6001 gtatgacgat tttaagtatt cgtcatactg attacctgtt ctggatcaat agatgggcag
     6061 tcgggtgggc ggatcagtta actgaatcga tccattgcac actgtcggcg gtgagcgtaa
     6121 agggggtagg ggtgcagatc gccaggctta aattgtctag ctgacaatgg ctgactgaca
     6181 gtgatgagtg ataggtgctg tctacgctga cgatttgcca ggcgctgccg ccacgctgtt
     6241 taacgatggc ttctttgcgc gtccagatgc gccagaacat ttccagttgc tgatcaggat
     6301 gcacggcgtc catctcggca tgttccccaa ggctgaatac ggcgttcgcc agccagcgcc
     6361 agttggcgcg cgggcgaatc acttcgatat cgcagccaac ttcgccttca tcgctcaaca
     6421 gcagggcgat atcgtcaccg ctatggctta agttgaacca tagcggcgtt tccggcgcga
     6481 atgccggttt gccttgttcg ccatagatga tctccggtag cggggaaagc gtgtgcgaaa
     6541 gcaatgcacg ccccgccagc cagcgttcgc gtcgtggacc ttgcggtgct tgctcgcgta
     6601 aagccggtgg cagtggagct gcgcttaagg tcgaaacttt ccccagaact atccgataca
     6661 tatcagggcc aacgtttaat ggaaaatgaa agtgcgtatc gtaacacttg tcgcctcatc
     6721 ccggtaaccg acttttcggt ctgcctggcc ccagtaaaat cgccagtttg ctaccgcctt
     6781 tggtggtttc catccagatt ttacacacgc tggttaatgg caccgaaagt agcatcccta
     6841 ccgggccgag cagccatccc caaattaaca atgaaagaaa taccaccatg gtggacatcc
     6901 ccagacgatg gcccatcatc cgtggttcta aaatattgcc gatgaccata tggaccacta
     6961 aaaacaatgc gccgaccaga atacattcgt aaataccatt aaacagcagc acctgaatca
     7021 ttggcggtac ggcggaaatt accgcaccga tattgggcac gtagttgagc aaaaacgcca
     7081 gtaccgccca catcagcgca aactgtacgc ccataagttc cagccccagc cagacgataa
     7141 cgcctgtcca taaacttagc agtgttttca atgccagata atgcgaaacg cctttaagtg
     7201 cgcggtgtag tccggcgatg tgaatttgtg gattattcaa cgcgaaacgc attttgtaag
     7261 ggacgtggcg cacttcaaac agcataaaaa ctacggtcat caccagcaaa agcacgctcg
     7321 ccattgcccc ggaaagcccg gtcattagcg ctgtggtgaa ggtaaccact ttttccgagt
     7381 ccatccgctg cagcattcgc tccggtgaca tatgcaaatt aagaaaaggc aacatctcct
     7441 gcaatttaaa aagtttgcgc gtcagctcct tattaaactt cggcagcata gagataaatt
     7501 cgttaaacga tgccgccagt acgccgacca gcgcagttag tgcgatcagc atcaccacca
     7561 ccacaatcgt aatggcaacg gggcgttgta ctccccgacg aataaaccag gtgacgagcg
     7621 ggttgaggac gatggcaaaa aacagcgcca gtaacagctg cacaatgata tctgccgctg
     7681 cgtgaatgcc cgcgaggatc actaccagcg aggccagctt gagcagaatg tgcatgcccg
     7741 ttttatcggg ttgaggggtt tccattgggg cttccttgtg actttttgta ttaagtgtag
     7801 cgggagtcac gccagcaata ttctgattct tactgctgat tttcacgtca gcacgtggta
     7861 aaaatgaaac actgttgtaa aaatgtggtg atcctcatgc ccgaacccgt agccgaaccc
     7921 gcgctaaacg gattgcgcct gaatttgcgc attgtctcca ttgtcatgtt taacttcgcc
     7981 agctacctca ccatcgggtt gccgctcgct gtattaccgg gctatgtcca tgatgtgatg
     8041 ggctttagcg ctttttgggc aggattggtt atcagcctgc aatatttcgc caccttgctg
     8101 agccgccctc atgccggacg ttacgccgat ttgctgggac ccaaaaagat tgtcgtcttc
     8161 ggtttatgcg gctgcttttt gagcggtctg ggatatctga cggcaggatt aaccgccagt
     8221 ctgcccgtca tcagcctgtt attactgtgc ctggggcgcg tcatccttgg gattgggcaa
     8281 agttttgccg gaacgggatc gaccctgtgg ggtgttggcg tggttggctc gctgcatatc
     8341 gggcgggtga tttcgtggaa cggcattgtc acttacgggg cgatggcgat gggtgcgccg
     8401 ttaggtgtcg tgttttatca ctggggcggc ttgcaggcgt tagcgttaat cattatgggc
     8461 gtggcgctgg tggccatttt gttggcgatc ccgcgtccga cggtaaaagc cagtaaaggc
     8521 aaaccgctgc cgtttcgcgc ggtgcttggg cgcgtctggc tgtacggtat ggcgctggca
     8581 ctggcttccg ccggatttgg cgttatcgcc acctttatca cgctgtttta tgacgctaaa
     8641 ggttgggacg gtgcggcttt cgcgctgacg ctgtttagct gtgcgtttgt cggtacgcgt
     8701 ttgttattcc ctaacggcat taaccgtatc ggcggcttaa acgtagcgat gatttgcttt
     8761 agcgttgaga taatcggcct gctactggtt ggcgtggcga ctatgccgtg gatggcgaaa
     8821 atcggcgtct tactggcggg ggcagggttt tcgctggtgt tcccggcatt gggcgtagtg
     8881 gcggtaaaag cggttccaca gcaaaatcag ggggcggcgc tggcaactta caccgtattt
     8941 atggatttat cgcttggcgt gaccggacca ctggctgggc tggtgatgag ttgggcgggc
     9001 gtcccggtga tttatctggc ggcggcggga ctggtcgcaa tcgcgttatt actgacgtgg
     9061 cgattaaaaa aacggcctcc ggaacacgtc cctgaggccg cctcatcatc ttaaaactta
     9121 ctgaataacc agcgtattaa tgatgttttc tgcggtggtc tgcgcttttt gctgattgtc
     9181 agctggcagc gtaatttgca tggtcagcag ttgattaccc acgttaccca gaataacgga
     9241 agagtacgcc gtctggcctt tcgcggagat aatgctgtct aactgctgca ttttgtgacc
     9301 tttcagctca atggctttat tggttaccac ttgcagctgc ggatcgcggc tacgttgctg
     9361 atcttccaga cgcttcgcca acaccgccag atcttctttc ggatcatcgc ccatgatgac
     9421 gatgactgct ttttgcccag tggcgtcgga ccagacatgc atgttattgg cctgcgttcc
     9481 cagcttaccg ctctggtcgg tcatatccgc tggcagcgag aaacttaact tgccatcaag
     9541 caggttgacg ggattcccgg tagcgttact ttccgcgacc gacccctgcg ccgtagcgtt
     9601 agtgtcttta tcatcacagg ccgcaagccc cataaccagc aggccaattc cgacatattt
     9661 aaccagattg cgcattgact tcttcctttc gataaacggc cataacggct cattcatcca
     9721 tcttatcaca actctgataa cgaaccttta actcgcctgc aaagcgttga tttcagattt
     9781 atctgccagt cttttcaaca gcatattgag taatacgcca tacattggca ggaagaaaac
     9841 gatactgatt aacactttga aacagtaatc gaccagcgcg atttccatcc agtgttcagc
     9901 cataaaggca tccgggctac gccagaaggc aatgaagaag aaggccagcg tgtcgctgac
     9961 gttaccgaac agtgtggacg ctgtcggtgc cagccaccag tgacgactct gacgcaggcg
    10021 gttaaaaacg tgcacgtcga ggatctgccc cagcgcgtag gccatgaaac tggcggtggc
    10081 gatacgggcg acaaacaggt tgaagtgggc gagcgcgccg aatccctgcc aggaacccat
    10141 atagaacagc gacgagatga cgtaggagat cagtaacgcc gggatcatca ccgcaaagat
    10201 gatacgacgg gccagcggtg cgccaaaaat acgcacggtc aggtcggtag caaggaaaat
    10261 aaacggaaag ctaaatgcgc cccaggtggt atggaaacca aaaatggaga cgggaagctg
    10321 aaccagatag ttactggagg tgatcaccag cagatgaaat aacgataacc agaacaacgc
    10381 cttatagcgt tgagtttgcg agaaaacgtt catattgtac ctttttgatt aaccattggg
    10441 gtgagggaac ccaatacgta cgacacgcta ttttaccttg attaaataac gttcttaccc
    10501 ttttttcgag ccgccgcatg atactgcttt acgttgacaa tgcaatggtt aattttcacg
    10561 caatcgttaa cctggtttgc ttacggactc acagggcgta aactagcgcc gtttttttat
    10621 gtgatgagaa gaaaatgacc gatctctttt ccagccctga ccacacactc gacgcacttg
    10681 gcctgcgctg cccggaacca gtaatgatgg tgcgcaaaac agtgcgcaat atgcagccag
    10741 gcgaaacgtt gctgattatc gccgacgatc cggccactac ccgcgatatt cctgggtttt
    10801 gtacctttat ggaacacgaa ctggttgcta aagagacgga tgaactgcct tatcgttatt
    10861 tgattcgcaa aggcggttga tggggaaaga ttggcttcga tgccgccttt tcccctcacc
    10921 ctaaccctct ccccagagga gcgaggggac cgatcgcgct caatgttgcg atcggtttgc
    10981 cttatctcct gcgcaacaat cttaacgcat tcgctgtcac cagcaccgtc gcacccgtat
    11041 ctgccagcac cgccagccat agcccggtca ttcctaacag tgtggtgacg aggaagatcc
    11101 ctttcagccc cagcgcaata gtgatgttct ggcggatatt ggcgtgagtg gcgcgtgcca
    11161 gttcaatcat ttgcaccagg ccgcgcaggt ggttatgggt taatgctgca tcggcggttt
    11221 ccagcgccac gtctgtgcca ctgcccattg caatcccgat ggcggcggct ttcatcgctg
    11281 gcgcgtcgtt aataccgtca ccgaccatcg ccagcggcgc atgttgattc agctcggtca
    11341 ccgctttgac tttatcttcc ggcaacaggc ccgctttaaa ctctagcccc agttccccgg
    11401 cgattgccgc cgctgcgcgt ggattatcgc cggtgaggat cacccctttg acgcccagcg
    11461 cgttcagttc actgatggca gttgcagcat cggcgcgcag ggtatcctgt aacgcaatga
    11521 caccaagcac gtcatcgtta cgtactacca gcactaccgt ttgcccggcg ctttccagtt
    11581 cgttaattaa accagcaaat gcatcagcgg gatgtttccc ggcagcgcat atcaacacgc
    11641 gctcaccgtt aacctgcgct tcaatgccag acccaaccag cgcccgctgt gattgggcgg
    11701 cgggaatggc gagttcagca acctgtgctt ctcgcacgat ggcttgcgcc agtggatgcg
    11761 tcgcgccttg ctcgaccgcc gctgccagtg tcagcagttc ggattcacta atacccgttg
    11821 ccggatgaat cgcggtaacg cgcggtttac cgacggtcag cgtaccggtt ttatcaaacg
    11881 ccacctgggt aacacgaccc agttgttcca gcgccgctcc gcctttaatt aacgccccac
    11941 gacgcgctgc tgctgccagc ccggaggtaa tcgccgcagg cgttgagata actaacgcac
    12001 acgggcagcc aatcagcagc agcgtcagcc ctttataaat ccactcttgc cagctggcgg
    12061 caaacagcag tggtggcacc agcgttacca gcagagcgac ggccataatc gctggcgtgt
    12121 aaatacggct gaaacggtcg ataaaccgct caatgggagc gcgacgctct tcggcttctt
    12181 cgatcagttt cagaatccgg tcaatggcgc tggcacccgg ttctgacagc acttccagca
    12241 ttaccagacg gtctacgctg gtggcgcctg caggaacttt atcgcccgtc gcgcgctcca
    12301 caggaatgga ttctccggtc agggcgcttt catcaaaact ggcaaacggt gagagcagtt
    12361 taccgtcggc aggcaaacgc ccacctgccg caacttcaat cacatcgcca ggccgcaggc
    12421 tgtttattgc cacctcttca cgctcaccgt tacgcagacg cgtggcggtt tctggcttca
    12481 gcgccattaa cgcgctgacc ccttgacgcg cgcgactggc ggcccagccc tccagtcgtt
    12541 cgccaatcaa aaacagcagc aacaccatcg cagcttcagc cgttgcgcca ataaacagcg
    12601 caccaatagc ggctacgctc attaacgttt caatggcgaa gtagctgccg gatttgatca
    12661 gccgtaatgc ctgacgagca atcgggtaca gcccaaccag cgtggtcgcg ataaacgcca
    12721 gttgcccgaa cggatgattg aactgctcca gaccccagct gattgccatc atcacgatta
    12781 gcgtaatcag cggcagattc tctttcaggc gtgatgcttg tggttcgtcg gcggactgtt
    12841 catcgcgcag ggaatagcct gctttttgca ccgcagattc aacctgggca cggatgtcat
    12901 tgtcggcatc gaccaccagt ttttcggtgg cgaacaacac ctgcacctga ttcacgcctg
    12961 caagctggcg cacggcattt tctaccttgc gcgcacaggc ggcgcagtcc atgccgctga
    13021 ctttccagct atagcgggtg ccggagacgt tttcagagag agttggcgtg ctggaacatg
    13081 cgccgtcgca gcaacagtcg ttggcgttct gtaccgtggt tagcggtttg aacgcagcaa
    13141 attgaggggc tttcttgccg tgattgtcag gagtcgacat ggcatcctcc ggttaagttt
    13201 tttctcatta accgaaggat acactctgga gtcgactcca gagtcaagtt ttatcagaga
    13261 tacagcgagc ggacgatgag gaaatgaccg gcaaagtagc aggccgcagc aatcgcgtta
    13321 tctgcccgga agcggcggcg atagtggcta cccagccaga caaagttact gataaacagc
    13381 aacgaagcgc cgacaaacgc agagagcgcc ggagctgtcg gacggaagaa ccacagttca
    13441 cctgccagcc acaccatcac cagcgtcatg ccgataaacg tgcagatagg ccaacgatac
    13501 tcttccagac gcgtccagat aatcgccagt aacagcgcac ccagaaccag caacaccagt
    13561 ggcagaggcc agaagaaaga gagcgtcatc tgactggcaa aatagatggt gtacagcagg
    13621 tgcgagagga aaaacgcgcc gatggcgtac atcagacgtt gacgtggcaa cagggttagc
    13681 gcatcgccca gcagtgaggc gcacagccct gccagcacca gatagctaat ggcgtcgaac
    13741 atcggcgctt gccaggccag taacagcagg agaagaaggg ttaacggttt aaacacccag
    13801 cgttgccagg ttggcccacg atacgatgca tccacagata gccatgcgga aagacagaca
    13861 gcgataaacg accaaagcat cttagttcct tgaattcgtt attttttcgt aagcggaatg
    13921 cctacggtct tgcttccagt ttagtggcga gggtggacgg atgacaacac cataactggc
    13981 agggtatgct tatttccgca ttttccgatg agggataaag atgagcaaac cacctctttt
    14041 ctttattgtt atcattggct taattgtcgt cgccgcatcg tttcgtttta tgcaacagcg
    14101 acgggaaaaa gctgataatg atatggctcc gctccagcaa aagctggtgg tggtgagcaa
    14161 caagcgggaa aaaccgatta acgatcgccg ttcgcgccag caggaagtga ctccggcagg
    14221 caccagtatg cgctacgagg caagcttcaa accgcaaagc ggaggaatgg agcagacgtt
    14281 tcgcctcgac gctcagcagt accatgccct gacagtgggc gatataggta cgctgagcta
    14341 taaaggaacg cgctttgtta gctttgtagg cgaacaataa ggttattttt taactttaaa
    14401 tttcttctgc cagaccagca gttcaaacac gccgaaaagg aaaatccgca ccttttcggc
    14461 ggtggtcatc tgcgggccat ctttaggtaa agtggatttc aatagcgcca gctgcatacc
    14521 atgcatcagc acggtaaaaa tcagcgccac gttaacgaaa atattcagcg ggcgcgggaa
    14581 gggatgcacc agattgagga ttaaaaatcc ccaaacgcag agcattaaca aacgaccaat
    14641 attaatcagc atcactttct ccttgtgctt cgcgttgata cagccgataa gccacctgac
    14701 ccgccacttt ttcccgatgt aatgaccagt ttgccggaac agtgggcaga ccgttttcga
    14761 cttcgctttc gacataaatc agggcttcgt cagccagcca gccgttatct tccagtaaat
    14821 ttatcgtctc ttctaacaag ccacggcgga acggtggatc gacaaacacg atattatgcg
    14881 gtgtgccttt ttgcgccagg aatgacattg cgttgctgtt caccacgcgt gcattgcctg
    14941 cttttagtgt cgccagattc ttaattaact gctgagaaac cgcgcgatcc atctcaatca
    15001 acgtggcccc cgcagcatag cgcgacaacg cttccagccc cagcgcgccg ctcccggcga
    15061 agcaatccag acattgggcg tcaacaatga ccggagccag ccagttaaac aacgtttcgc
    15121 gtacgcggtc ggtggtgggg cgcagacctg ggctatcagg aaccgggagt ttacggcctc
    15181 gccactgccc gccaataatg cggatttggc cgctgccgga atgattcggt tttttcatga
    15241 taaattgctc aatccgccag ataacacata cttgcaggcg gtgatgtgaa ttaagttaag
    15301 tgatagacta tttcatcatt tttttagctg ctatgtacat agcgttaacg ctgtgccatg
    15361 aagcaacagc gaggagtgta gtcgcaaatg gcgaaagaaa aaaaacgtgg ctttttttcc
    15421 tggctgggct ttggtcaaaa agagcagacc ccggaaaaag agacagaagt tcagaatgaa
    15481 caaccggttg tagaagaaat cgttcaggcg caagagcctg cgaaggcttc ggaacaagcc
    15541 gttgaagagc agccgcaggc gcatactgag gccgaagcgg aaacttttgc tgccgacgtt
    15601 gtggaagtca ctgaacaggt tgttgaaagt gaaaaagcgc aacctgaagc ggaagttgtt
    15661 gcacagccgg aactggtcgt agaagaaacg ccggagccag tggctatcga gcgtgaagag
    15721 ctgccgttgc cggaagacgt caacgccgaa gaggtttcgc cagaagagtg gcaggccgaa
    15781 gcggaaaccg tagagattgt cgaagcggcg gaagaagaag cggctaaaga agaaatcacc
    15841 gacgaagagc tggaagcaca ggcgctggct gccgaagcag cagaagaggc ggtgatggtg
    15901 gttcctccgg cagaagaaga gcagccggta gaagaaatcg ctcaggagca ggaaaaaccg
    15961 accaaagaag gtttcttcgc gcgcctgaaa cgcagcctgt taaaaactaa agaaaatctc
    16021 ggttccggat ttatcagcct gttccgcggt aaaaaaatcg acgatgatct gtttgaagag
    16081 ctggaagaac agctgttgat cgccgatgtg ggcgtggaaa ccacacgtaa aataatcacc
    16141 aatctgacgg aaggcgcatc ccgcaagcag cttcgtgatg ccgaggcgct ttatggcctg
    16201 ctgaaagaag agatgggcga gattctggcg aaagtcgatg agccgctgaa tgttgaaggt
    16261 aaaacgccgt tcgtgatcct gatggtgggc gtcaacggtg tgggtaaaac cacgacgatt
    16321 ggtaagctgg cacgtcagtt tgagcagcag ggtaaatcgg tgatgctggc ggcgggcgat
    16381 actttccgtg cggcagcggt tgaacagctt caggtctggg gtcagcgcaa caatattccg
    16441 gtgattgccc agcataccgg ggcggattcc gcctctgtta tcttcgacgc cattcaggca
    16501 gctaaagcgc gtaatatcga cgtcctgatt gccgataccg ccggacgcct gcagaacaaa
    16561 tcgcacctga tggaagagtt gaagaaaatc gtccgcgtga tgaagaaact cgacgttgaa
    16621 gcgccgcatg aagttatgct gactattgat gccagcaccg ggcagaacgc ggtaagccag
    16681 gccaaactgt tccatgaagc cgttggctta accggcatca cgctaacgaa actggacggc
    16741 acggcgaaag gcggagtaat tttctcggtg gctgaccagt ttggtatccc tatccgctac
    16801 attggtgtcg gcgaacgtat tgaggatttg cgtccgttta aggcggacga ctttatagag
    16861 gcactttttg cccgagagga ttaacaatga ttcgctttga acatgtcagc aaggcttatc
    16921 tcggtgggag acaggcgctg cagggcgtta cgttccatat gcagccgggt gagatggcgt
    16981 ttctgaccgg tcattccggc gcagggaaaa gtaccctcct gaagctgatc tgtgggattg
    17041 agcggcccag cgccgggaaa atctggttta gcggccatga catcacgcgt ctgaaaaacc
    17101 gtgaagttcc gtttctgcgc cgccagattg gtatgatttt ccaggatcac catctactga
    17161 tggaccgtac tgtctacgat aacgtggcga tcccgctgat tatcgccggt gccagcggtg
    17221 acgatattcg tcgccgggtg tcggcggcac tggataaagt cgggctactg gacaaagcga
    17281 agaactttcc tattcagctt tccggcggtg aacaacagcg tgttggcatt gcccgcgcgg
    17341 tggtgaataa gcccgcggta ctgctggcgg acgaaccgac tggtaacctg gacgacgcgt
    17401 tgtcggaagg cattttacgt ctgtttgaag agtttaaccg cgttggggta accgtattga
    17461 tggcaacgca cgacatcaac ctgatttcgc ggcgttccta tcgcatgctc accctgagcg
    17521 atggtcactt gcatggaggc gtgggccatg aataagcgcg atgcaatcaa tcatattcgg
    17581 cagtttggcg ggcgtctgga tcgcttccgt aaatcggtcg gcggctcagg cgacggcggt
    17641 cgtaacgcac cgaaacgcgc gaaatcctcg ccaaaaccgg taaatcgcaa aaccaacgtt
    17701 ttcaacgaac aggtgcgcta tgccttccac ggcgcattgc aggatctgaa aagcaaaccg
    17761 ttcgccacgt ttttaacggt gatggttatc gccatctccc tgacgctgcc cagcgtctgt
    17821 tatatggtgt acaaaaacgt taaccaggcg gcgacgcaat attatccgtc accgcaaatc
    17881 actgtttatc tgcaaaaaac gctggacgat gacgccgctg cgggtgtggt ggcacagtta
    17941 caggccgagc aaggcgtaga gaaagtgaat tatctttctc gtgaagacgc actgggcgag
    18001 ttccgtaact ggtctggttt tggtggtgcg ctggatatgc tggaagaaaa cccgcttccg
    18061 gcagtggcgg tggtgatccc gaaactcgat tttcagggga cggaatcact gaatacgctg
    18121 cgtgatcgta tcacccagat caacggcatt gacgaagtgc ggatggatga cagctggttt
    18181 gcccgtctgg cggcgttaac cgggctggtc gggcgcgttt ccgcgatgat cggcgtgttg
    18241 atggtggcag cagtgttcct cgtcatcggc aacagtgtgc gtctgagcat ctttgctcgc
    18301 cgtgactcca ttaacgtgca gaaactgatt ggtgcgacgg atggtttcat cctgcgcccg
    18361 ttcctgtacg gaggcgcatt gctgggattt tctggcgcat tgctgtcatt aattttgtca
    18421 gaaattctgg tactgcgttt gtcatcggcg gttgcggaag tggcacaggt tttcggaaca
    18481 aagtttgata tcaatggctt atcgttcgat gaatgcctgc tgttgctgct ggtatgctcg
    18541 atgattggct gggtggcagc gtggcttgcc acggtacaac atttacgcca ctttacgccg
    18601 gaataataaa agcgtgttat actctttccc tgcaatgggt tccgtagcag ggaaagagac
    18661 cccgttgtct cttcccggta tttcatctct atgtcacatt ttgtgcgtaa tttattcaca
    18721 agcttgcatt gaacttgtgg ataaaatcac ggtctgataa aacagtgaat gataacctcg
    18781 ttgctcttaa gctctggcac agttgttgct accactgaag cgccagaaga tatcgattga
    18841 gaggatttga atgactgaca aaatgcaaag tttagcttta gccccagttg gcaacctgga
    18901 ttcctacatc cgggcagcta acgcgtggcc gatgttgtcg gctgacgagg agcgggcgct
    18961 ggctgaaaag ctgcattacc atggcgatct ggaagcagct aaaacgctga tcctgtctca
    19021 cctgcggttt gttgttcata ttgctcgtaa ttatgcgggc tatggcctgc cacaggcgga
    19081 tttgattcag gaaggtaaca tcggcctgat gaaagcagtg cgccgtttta acccggaagt
    19141 gggtgtgcgc ctggtctcct tcgccgtcca ctggatcaaa gcagagatcc acgaatacgt
    19201 tctgcgtaac tggcgtatcg tcaaagttgc gaccaccaaa gcgcagcgca aactgttctt
    19261 caacctgcgt aaaaccaagc agcgtctggg ctggtttaat caggatgaag ttgaaatggt
    19321 ggcccgtgaa ctgggcgtaa ccagcaaaga cgtacgtgag atggagtcgc gtatggcggc
    19381 acaggacatg acctttgacc tgtcttccga tgacgattcc gacagccagc cgatggctcc
    19441 ggtgctctat ctgcaggata aatcatctaa ctttgccgac ggcattgaag atgataattg
    19501 ggaagagcaa gcggcaaacc gtctgaccga cgcgatgcaa ggtctggacg agcgtagcca
    19561 ggatatcatc cgcgcgcgct ggctggatga agacaacaag tccacgttgc aggaactggc
    19621 tgaccgttac ggtgtttccg ctgagcgtgt gcgccagctg gaaaagaatg cgatgaaaaa
    19681 acttcgcgct gccattgaag cgtaatttcc gctactcagc agagaaccct ggatgagaat
    19741 ccggggtttt tgttttttgg gcctctgcaa taatcagttt ccccctccgg caaaacgcca
    19801 atccccacgc agattgttaa taaactgtca aaatagctat tccaatatca taaaaatcgg
    19861 gtatgtttta gcagagtatg ctgctaaagc acgggtagtc atgcataaaa cgaaataaag
    19921 tgctgaacaa caacatcaca acacacgtaa taaccagaag aatggggatt ctcaggatga
    19981 acataaaggg taaagcgtta ctggcaggat gtatcgcgct ggcattcagc aatatggctc
    20041 tggcagaaga tattaaagtc gccgtcgtag gcgcaatgtc cggtccggtg gcgcagtacg
    20101 gtgaccagga gtttactggc gcagagcagg cggttgcgga tatcaatgct aaaggtggta
    20161 tcaaaggcaa caaactgcaa atcgtaaaat atgacgatgc ctgtgacccg aaacaggcgg
    20221 ttgcggtggc gaacaaagtc gttaacgacg gcattaaata cgtgattggt cacctctgct
    20281 cttcatcaac gcagccagcg tctgacatct acgaagacga aggcatttta atgatcaccc
    20341 cagcggcaac cgcaccagag ctgaccgccc gtggctatca gctgatcctg cgcaccaccg
    20401 gcctggactc cgaccagggg ccgacggcgg cgaaatatat tcttgagaaa gtgaaaccgc
    20461 agcgtattgc tatcgttcac gacaaacagc aatacggcga aggtctggcg cgagcggtgc
    20521 aggacggcct gaagaaaggc aatgctaacg tggtgttctt tgatggtatc accgccgggg
    20581 aaaaagattt ctcaacgctg gtggcgcgtc tgaagaaaga gaatatcgac ttcgtttact
    20641 acggcggtta tcacccggaa atggggcaaa tcctgcgtca ggcacgcgcg gcagggctga
    20701 aaactcagtt tatggggccg gaaggcgtgg ctaacgtctc gctgtctaac attgcgggtg
    20761 aatcagcgga aggactgctg gtgaccaagc cgaagaacta cgatcaggtt ccggcgaaca
    20821 aacccattgt tgacgcgatc aaagcgaaga aacaggaccc aagcggcgcg ttcgtctgga
    20881 ccacctacgc cgcgctgcaa tctttgcagg tgggcctgaa tcagtctgac gatccggctg
    20941 aaatagccaa atacctgaaa gcgaactccg tggatacggt gatggggccg ctgacctggg
    21001 atgagaaagg tgatctgaaa ggctttgagt tcggcgtatt tgactggcac gccaacggca
    21061 cggcgactga tgcgaagtaa tcattaatcg gcaactttgg gttgccgcca aattgctaat
    21121 atcgagtacg ttgcttcatg ccggatgcgg cgtaaacgcc ttatccggcc tacaagattc
    21181 aaagaaatca gtaaattgca gcacacattg taggcctgat aagcgtagcg catcaggcaa
    21241 tatcacttgt gaaatcaaac ttgacgatta acgcttctcc cagccgccct gttgtgccgt
    21301 aaatcccagt gcctgcataa acgctgtcat cacgccgcga tcttccacgc ctgcatccgc
    21361 catccaccag catgaaacgc caggattgtt acgcaaaacc tcttccagca gatattgccc
    21421 cacaccgcga cggcgggtga cttccctcac acgcagggaa tccagtgccc cctcggtgcc
    21481 gcttaaggtt acccgcacgg ccgcgagaag gcgctcgtta aaacgcgcgg cgtagatacg
    21541 gtggttatcg tcaacctgta aagaggaagg ggaatactcc ggccagatct tttgcaggtc
    21601 aatccggtcc tggtcgctaa agttttctaa tcgaatgatg gtcagcttca tggggaaccc
    21661 gtgtaaatca caaaagtgga accagtgtag cgaaataatt taatcggagg ctttctcttt
    21721 tttaattctt ttggcaggtg attaattttt taacagcaat aattacaaag ttaaaacatt
    21781 agagaatgaa aaatacccag cataatcccc tgaatggtag tgaattattc cgccctttgt
    21841 accgttattt tatgctgaca acggcgcttt tttctgttta tctatcaata aattcagaat
    21901 attatctgtt cttaatcgac tgaaaaatag ggattttaat cgctatcatc acaaaatact
    21961 gtgctaaacc cttaatcaga caggcaaaaa cagtgcagta taaaaaaaga acagtctgat
    22021 ttgttaacac ataagaacaa agcaacacaa catcacgaat ggggattttt gactatgaaa
    22081 cggaatgcga aaactatcat cgcagggatg attgcactgg caatttcaca caccgctatg
    22141 gctgacgata ttaaagtcgc cgttgtcggc gcgatgtccg gcccgattgc ccagtggggc
    22201 gatatggaat ttaacggcgc gcgtcaggcg attaaagaca ttaatgccaa agggggaatt
    22261 aaaggcgata agctggttgg cgtggaatat gacgacgcct gcgacccgaa acaagccgtt
    22321 gcggtcgcca acaaaatcgt taacgacggc attaaatacg ttattgggca cctgtgttcc
    22381 tcctctaccc aacctgcatc agatatctac gaagacgaag gtattctgat gatttcgccg
    22441 ggagcgacca atccggagct gacccaacgc ggttatcaat acatcatgcg tactgccggt
    22501 ctggactctt cccaggggcc aacggcggca aaatacattc ttgagacggt gaagccccag
    22561 cgcatcgcca tcattcacga caaacaacag tatggcgaag ggctggcgcg ttcggtgcag
    22621 gacggattga aagcagccaa tgccaacgtc gtcttcttcg acggcattac cgctggtgag
    22681 aaagatttct ccgcgctgat cgcccgcctg aaaaaagaaa acatcgactt cgtttactac
    22741 ggcggttact acccggaaat ggggcagatg ctgcgccagg cccgttccgt tggcctgaaa
    22801 actcagttta tggggccgga aggggtgggt aatgcgtcgc tgtcgaacat tgctggtgat
    22861 gccgccgaag gtatgttggt cactatgcca aaacgctatg accaggaccc ggcaaacaaa
    22921 ggcatcgttg atgcgctgaa agcagacaag aaagatccgt ccggacctta tgtctggatc
    22981 acctacgcgg cggtgcaatc tctggcgact gcccttgagc gtaccggcag cgatgagccg
    23041 ctggcgctgg tgaaagattt aaaagctaac ggtgcaaaca ccgtgattgg gccgctgaac
    23101 tgggatgaaa aaggcgatct taagggattt gattttggtg tcttccagtg gcacgccgac
    23161 ggttcatcga cggcagccaa gtgatcatcc caccgcccgt gaaatgcggg cggttttaga
    23221 aaggttacct tatgtctgag cagtttttgt atttcttgca gcagatgttt aacggcgtca
    23281 cgctgggcag tacctacgcg ctgatagcca tcggctacac catggtttac ggcattatcg
    23341 gcatgatcaa cttcgcccac ggcgaggttt atatgattgg tagctatgtc tcctttatga
    23401 tcatcgccgc actgatgatg atgggcattg ataccggctg gctgctggtg gcggcgggat
    23461 tcgtcggcgc aatcgtcatt gccagcgcct acggctggag tatcgaacgg gtagcctatc
    23521 gtccggtgcg taactctaag cgcctgattg cgcttatttc cgcaatcggc atgtctatct
    23581 tcctgcaaaa ctacgtcagc ctgaccgaag gttcgcgcga cgtggcgctg cccagcctgt
    23641 ttaacggtca gtgggtggtg ggccatagcg aaaacttctc tgcctctatt accaccatgc
    23701 aggcggtgat atggattgtt actttcctcg ccatgctggc gctgacgatt ttcattcgct
    23761 attcccgtat ggggcgcgcc tgccgtgcct gcgcggaaga tctgaaaatg gcgagcctgc
    23821 ttggcatcaa caccgaccgg gtgattgcgc tgacctttgt gattggcgcg gcgatggcgg
    23881 cagtggcggg cgtgctgctc ggtcagttct acggcgtaat taacccttac atcggcttta
    23941 tggccgggat gaaagccttt accgctgcgg tgctcggtgg gattggcagc attccggggg
    24001 cgatgattgg cgggctgatc ctggggattg cggaggcgct ctcttctgcc tatctgagta
    24061 cggaatataa agatgtggtc tcattcgccc tgctgatcct ggtgctgctg gtgatgccga
    24121 ccggtattct gggtcgcccg gaggtagaga aagtatgaaa ccgatgcata ttgcaatggc
    24181 gctgctctct gccgctatgt tctttgtgct ggcgggcgtc tttatgggcg tgcaactgga
    24241 gctggatggc accaaactgg tggtcgacac ggcttcagag atccgttggc agtgggtgtt
    24301 tatcggcacg gcggtggtct ttttcttcca gcttgtgcgg cctgctttcc agaaagggct
    24361 aaaaagcgtt tccgggccga agtttattct gcccgcgatt gatggctcca cggtgaagca
    24421 gaaactgttc ctcgtggcgc tgctggtgct tgcagtggcg tggccgttta tggtgtcgcg
    24481 tgggacggtg gatatcgcca ctctgaccat gatctacatt attctcggtc tcgggctgaa
    24541 cgtggttgtt ggtctttctg gtctgctggt gctgggatac ggcagctttt acgccatcgg
    24601 cgcttacact tttgcgctgc tcaatcacta ttacggtctg ggcttctgga cctgtctgcc
    24661 gattgccggg ttgatggcgg cggcggcggg cttcctgctc ggttttccgg tgctgcgtct
    24721 gcgcggtgac tatctggcga tcgttaccct cgggttcggc gaaattgtgc gcatattgct
    24781 gctcaataac accgaaatta ccggcggacc gaacggtatc agtcagattc cgaaaccgac
    24841 gctcttcgga ctcgagttca gccgtaccgc ccgcgaaggc ggctgggaca cgttcagtaa
    24901 tttctttggc ctgaaatacg atccctccga tcgcgtcatc ttcctctacc tggtggcgtt
    24961 gctgctggtg gtgttaagcc tgtttgtcat taaccgcctg ctgcggatgc cgctgggccg
    25021 tgcgtgggaa gcgttgcgtg aagacgagat cgcctgccgt tcgctgggct taagcccgcg
    25081 tcgtatcaag ctgactgcct ttaccatcag cgccgcgttt gctggttttg ccggaacgct
    25141 gtttgctgcg cgtcagggct ttgtcagccc ggaatccttc acttttgccg aatcggcgtt
    25201 tgtgctggcg atagtggtgc tcggtggtat gggttcgcaa tttgcggtga ttctggcggc
    25261 aattttgctg gtggtgtcgc gagagttgat gcgtgatttc aacgaataca gcatgttaat
    25321 gctcggtggt ttgatggtgc tgatgatgat ctggcgtccg cagggcttgc tgcccatgac
    25381 gcgcccgcaa ctcaagctga aaaacggcgc agcgaaagga gagcaggcat gagtcagcca
    25441 ttattatctg ttaacggcct gatgatgcgc ttcggcggcc tgctggcggt gaataacgtc
    25501 aatcttgaac tgtacccgca ggagatcgtc tcgttaatcg gccctaacgg tgccggaaaa
    25561 accacggttt ttaactgcct gaccggtttc tacaaaccca ccggtggcac tattttactg
    25621 cgcgatcagc atctggaagg tttgcccggc cagcaaatcg cccgcatggg cgtggtgcgc
    25681 accttccagc atgtgcgtct gtttcgtgaa atgacggtga ttgaaaactt gctggtggcg
    25741 cagcatcagc aactgaaaac cgggctgttc tccggcctgt tgaaaacgcc ttctttccgc
    25801 cgcgcccaga gcgaagcgct cgaccgtgcc gcaacctggc ttgagcgcat tggtttgctc
    25861 gaacacgcta accgtcaggc gagtaacctg gcctacggtg accagcgccg tctggagatc
    25921 gcccgctgca tggtaacgca gccggagatt ttaatgctcg acgaaccggc agcaggtctt
    25981 aacccgaaag agacgaaaga gctggatgag ctgattgccg aactgcgtaa ccatcacaac
    26041 accactatct tgttgattga acacgatatg aagctggtga tggggatttc agacagaatt
    26101 tacgtggtca atcaggggac gccgctggca aacggtacgc cggagcagat ccgtaataac
    26161 ccggacgtga tccgtgccta tttaggtgag gcataagatg gaaaaagtca tgttgtcctt
    26221 tgacaaagtt agcgcccact acggcaaaat ccaggcgctg catgaggtga gcctgcatat
    26281 caatcagggc gaaattgtca cgctgattgg cgcgaacggg gcggggaaaa ccaccttgct
    26341 cggcacgtta tgcggcgacc cacgcgccac cagcgggcga attgtgtttg atgataaaga
    26401 cattaccgac tggcagacgg cgaaaatcat gcgcgaagcg gtggcgattg tcccggaagg
    26461 acgtcgcgtc ttctcacgca tgacggtgga agagaatctg gcgatgggcg gcttttttgc
    26521 tgaacgcgac cagttccagg agcgcataaa gtgggtgtat gaactgtttc cacgtctgca
    26581 tgaacgccgt gttcagcggg caggcaccat gtccggcggt gaacagcaga tgctggcgat
    26641 tggtcgcgct ttgatgagta acccacgttt gttattgctt gatgaaccct cgctcggact
    26701 ggcaccgatt atcatccagc aaattttcga caccatcgag caactgcgcg agcaggggat
    26761 gaccatcttc ctcgtcgagc agaacgccaa ccaggcgctg aagctcgctg atcgcggcta
    26821 cgtgctggaa aacggtcatg tggtgctctc cgatactggc gatgcgctgc tggcgaacga
    26881 agcggtgaga agtgcgtatt taggcgggta ataatatttt gagtaaaaag gagtcgaatg
    26941 actccttttt tgtacttgtt agttgtacaa tttaattgta cttaatatgg aggatatcat
    27001 gcataccatg aactacagcg aagcgcggca gaaccttgca ttagttctgg aaacagcggc
    27061 gacaggtact ccggtaacga tcacgaggcg tgggcataag gcagctgtga ttatcagcgc
    27121 agaagagttt gaacgttacc agaaggctaa actggacgca gaatttgacg ccatcatggc
    27181 tattcatggc agcgaaatta aggagttagc ggataaatga ccattcagtt tatctcagcg
    27241 gaggagataa tttatttcca cgataaattg cttagcgtta caccaggtgt aacaggaatg
    27301 tccgatccag ggcgagcaga agcgcttttg taccgggtac tgaataagta tgagtatgaa
    27361 ggcgtttctg atctttggct gctggcggcg atgcatctat tggcaatctc gcgcggtcat
    27421 atctttaatg atggtaataa gcgcaccgca ctgtttatca cgctattatt ccttaaacgt
    27481 aacggcatca tacttgctgc aaacccagag tttgtagaat tgacggttga tgtggcggcg
    27541 ggaaaagtat cgttagaaga tatcgttacc cgcttgcgtc aacgcggtac ctgacaaggt
    27601 tcttagccat tctgcttttt atccgtgccc catctcccat tttccctcac ccacgccgtc
    27661 accgccttgt catctttctg acaccttact atcttacaaa tgtaacaaaa aagttatttt
    27721 tctgtaattc gagcatgtca tgttaccccg cgagcataaa aagcgtgaat tcgcgcatcc
    27781 ggtacaacaa gagagataaa cgatgaaacc gttacattat acagcttcag cactggcgct
    27841 cggactggcg ttaatgggga atgcacaggc agtgacgacc attccgttct ggcattctat
    27901 ggaaggggaa ctgggtaaag aggtggattc tctggcccaa cgttttaacg ccgaaaaccc
    27961 ggattacaaa attgtaccga cctataaagg caactacgaa cagaatttaa gcgcggggat
    28021 tgccgcattt cgtaccggca acgcgccggc tattttgcag gtttatgaag ttggcaccgc
    28081 caccatgatg gcgtcgaaag ccattaaacc ggtatatgac gtgtttaaag aggcggggat
    28141 tcagttcgat gagtcgcagt ttgtgccgac ggtttcaggc tactactccg acagcaaaac
    28201 cggccactta ctctcccagc cgttcaatag ctcgactcct gttctctatt acaacaaaga
    28261 cgccttcaag aaagccggtt tagacccgga acagccgccg aaaacctggc aggatctggc
    28321 ggactatgcc gcgaaactga aagcctccgg tatgaagtgc ggctacgcca gcggctggca
    28381 gggctggatc caactggaaa actttagcgc ctggaacggt ctgccgtttg ccagcaaaaa
    28441 caacggcttt gacggcacag acgcggtgct ggagttcaac aagccggagc aggtgaaaca
    28501 catcgctatg ctcgaagaga tgaacaagaa gggcgatttc agctacgtcg ggcgtaagga
    28561 tgaatccact gagaagttct ataacggtga ttgcgcgatg accaccgcct cttccggttc
    28621 gcttgccaac attcgtgagt acgccaaatt taactacggc gtaggcatga tgccttacga
    28681 cgccgatgcg aaagatgcac cgcaaaacgc cattatcggc ggggccagcc tgtgggtgat
    28741 gcagggcaaa gataaagaaa cgtataccgg cgtggcgaag ttcctcgact tcctcgcgaa
    28801 gccagaaaac gctgccgagt ggcatcagaa aaccggttat ctgccaatca ccaaagcggc
    28861 gtatgacctg acccgtgagc agggctttta tgagaaaaac ccaggggcgg ataccgcgac
    28921 gcgtcagatg ctgaataagc cgccgttacc gttcaccaaa gggctgcgtc tgggcaacat
    28981 gccgcagatc cgcgtgattg tggatgaaga gctggagagc gtgtggaccg gtaagaagac
    29041 accgcagcag gcgctggata ccgccgttga gcgtgggaac cagttactgc gccgctttga
    29101 gcagtctacg aagtcttaat tctcttaccc tcaccctaac cctctcccaa agggagaggg
    29161 gaccgatcgg cgtggtatcc tggtgccgtg tcggtttgca acttcgctcg gtgggaaagt
    29221 atgcaccgcc tttctctctc gcccctctgg ggagagggcc ggggtgaggg gaacacaaca
    29281 cgaagagttt aactatgtca tcatcccgtc cggtgttccg ctcgcgctgg ctgccctatt
    29341 tgctggtcgc gccgcagctc atcatcaccg ttatcttttt tatctggcct gcgggcgaag
    29401 cgttgtggta ctcgctgcaa agcgtcgatc cgtttggttt ctccagccag tttgtcggcc
    29461 tggataactt cgtcacgctg tttcatgaca gctactatct cgactccttc tggacgacga
    29521 taaaattcag cacctttgtc accgtcagcg gtttgctggt gtcgctgttc tttgcggcgc
    29581 tggtggagta catcgtgcgc ggcagccgtt tctatcaaac cttaatgctg ctgccttacg
    29641 ctgtggctcc cgccgttgcc gccgtattgt ggatcttcct gtttaacccc ggtcgcgggc
    29701 tgatcaccca ttttctcgct gagttcggct acgactggaa ccacgcgcaa aacagcggtc
    29761 aggcgatgtt tctggtggtg tttgcctcgg tgtggaagca aatcagctac aactttctgt
    29821 tcttctatgc cgcgctgcaa tccattcccc gttcgttgat cgaagccgca gccatcgacg
    29881 gtgcaggccc gattcgccgc ttctttaaga ttgcgctgcc gcttatcgct ccggtgagtt
    29941 tcttcctgct ggtggtgaac ctggtgtacg ccttcttcga taccttcccg gtgatcgacg
    30001 ccgccacgtc cggcgggccg gttcaggcca ccacgacgct gatttataag atttaccgcg
    30061 aaggctttac cggactggat ctggcttcgt ctgccgcgca gtcggtggtg ttgatgttcc
    30121 tcgtcatcgt gctgacggtg gtgcagttcc gctatgttga aagcaaggtg cgttaccaat
    30181 gattgagaac cgtccgtggc tgacaatatt cagccatacc atgctgatcc tcgggatcgc
    30241 ggtgatcctc ttcccgctgt acgtggcgtt tgtcgcggcg acactggata aacaagccgt
    30301 ctacgccgcg ccgatgacgc taatccccgg cacgcatctg ctggaaaaca tccacaacat
    30361 ctgggtgaac ggggtaggca cgaatagcgc gccgttctgg cggatgttgc ttaacagctt
    30421 tgtgatggcg ttcagcatta cgctcggcaa aattaccgtc tcgatgctct cggcctttgc
    30481 cattgtctgg tttcgttttc cactacgtaa cctcttcttc tggatgattt ttatcaccct
    30541 gatgctgccg gttgaagtgc gtatcttccc gacggtggaa gtcatcgcca acctgaaaat
    30601 gctcgacagc tacgcaggtt taacgctgcc gctgatggcc tcggcgaccg ccacgtttct
    30661 gtttcgccag ttctttatga ccctaccgga tgaactggtg gaagccgcgc ggatcgacgg
    30721 cgcgtcacca atgcgtttct tttgcgacat cgtttttccg ctgtccaaaa ccaatctggc
    30781 ggcgctgttt gtgatcacct ttatctacgg ctggaaccag tatttgtggc cgttgttgat
    30841 tattaccgac gttgatctcg gcaccaccgt ggcagggatt aaagggatga tcgccacagg
    30901 cgaaggcaca acggaatgga actcggtgat ggcggcgatg ttgttaaccc tgatcccacc
    30961 agtagtgatt gttttagtga tgcagcgtgc cttcgtgcgc ggcctggtcg atagtgagaa
    31021 ataagatggc aggactgaaa ttacaggcag taaccaaaag ctgggatggc aaaacccagg
    31081 tcattaaacc gctgaccctt gatgtggcgg atggcgaatt tatcgtgatg gtcgggccgt
    31141 ctggctgcgg gaaatcgacg ttattgcgaa tggttgccgg actggagcgg gtaacgacgg
    31201 gcgatatctg gatcgaccgc aagcgtgtca ccgaaatgga gccgaaagat cgcgggattg
    31261 cgatggtgtt ccagaactac gcgctttatc cgcatatgag cgtcgaagaa aacatggcgt
    31321 gggggctgaa aattcgcggc atgggcaagc agcaaattgc cgagcgcgtt aaagaagcgg
    31381 cgcgcattct ggagctggac ggcctgctta agcgccgccc gcgtgagcta tctggcggtc
    31441 agcgtcagcg tgtggcgatg gggcgtgcga ttgtgcgtga gccggcagtg ttcctgtttg
    31501 atgagccgct ctctaacctc gatgccaagt tgcgtgtgca gatgcgtctt gaactgcaac
    31561 agctgcaccg tcgcctgaaa acgacttcac tctacgttac ccacgatcag gttgaggcga
    31621 tgacgctcgc ccagcgagta atggtgatga acggcggcgt tgccgaacag attggcacgc
    31681 cagttgaagt gtacgaaaag cccgccagcc tgtttgtggc gagttttatc ggcagtccgg
    31741 cgatgaatct gctgaaaggg cgtgtgaata acgaaggtac gcatttcgaa ctggacggcg
    31801 gtatcgcgct gccgctaaac ggtggctacc gtcagtatgc tggacgtaaa atgactctcg
    31861 gcattcgccc ggaacatatt acgctacgtt cgcaggctga aggtggcgtg ccgctggtga
    31921 tggacacgct ggagatcctc ggcgcagata acctggcgca cggacgctgg ggcgaacaga
    31981 agctggtgtt gcgactggcg catcaggagc gcccgacggc aggcagcacg ctgtggctgc
    32041 atctgccgga aaatcagcta catctttttg atggtgaaac aggacaacga gtatgagtaa
    32101 ctggccttat ccccgcatcg tcgcccatcg tggcggcggt aaactggccc cggaaaacac
    32161 cctggcggca atcgatgtgg gggcaagata cggtcataag atgatcgaat ttgacgcgaa
    32221 gttatcgaaa gatggcgaga tcttcttgct tcatgacgac aatctcgaac gcaccagcaa
    32281 cggctggggc gtcgcgggtg aactgaactg gcaggattta ctgcgcgtgg atgcgggcag
    32341 ttggtacagc aaagcgttta aaggtgagcc gctgccgttg ctttcgcagg tggcggaacg
    32401 ctgtcgcgaa cacgggatga tggcgaatat cgaaatcaaa cccaccactg gcaccgggcc
    32461 attaacgggc aaaacggtgg cgctggcggc acgcgaactg tggaccggta tgacgccgcc
    32521 gctgctgtca tcgtttgaga ttgatgcttt agaagccgcg caacaggcag caccggaact
    32581 gccgcgtggt ttattgcttg atgaatggcg cgacgactgg cgagaattga ccgcgcggct
    32641 gggctgcgtc tctattcatc tcaatcataa gttgctcgat aaagcgcgag tgatgcagtt
    32701 gaaagacgcc ggattacgga ttctggttta taccgtcaat aaaccccagc gcgcggcaga
    32761 gttgctgcgc tggggcgtgg attgcatctg taccgatgcg attgacgtga ttggtccgaa
    32821 ctttacggcc caatagtttt caacggaatg tcaggctgcg gcgtgctggg ctgattcagc
    32881 atgtcgccgt ttctcctttc cggcagcata tgctgctgat tcaacgaact atccggatta
    32941 cggttgctgt ttaacatccc gccattggtg ttgggcagca tttgctgtcg ggcgggattt
    33001 cgctcccccg gctgtgactg caacacccgc tgagaattat tgtttatctg gttttctaaa
    33061 tgctgctgtt gcagctgcgt ttgcgttttc agttgctgat tcagcatccc tttttgctgg
    33121 atttgctgcg tttgcatttg ggtttgcatc cgctgctgac tggggatctg ataccccggc
    33181 tggttagggt tattcagagt attaatgggc tgtgcaaagc cgacaaacgg caggagtgcc
    33241 gtaagaatca gaagtcgttt catcgcgtat cctcctctga agatattctt taagtttact
    33301 cctttcctgc aatcatcagg attaattcag agttatatac caggcttagc tggggttgcc
    33361 ccttaatctc tggagaataa cgatgataaa accgacgttt ttacgccggg tggccattgc
    33421 tgctctgttc acaggaagtt gttttagtac ggtagccgcg ccacctgcgc cgtctcctgt
    33481 ttcttacggc gtggaggaag atgtctttca tccggtacgc gcgaaacagg ggatggtggc
    33541 gtctgtagat gctactgcca ctcaggtggg ggtggatatc ctcaaggagg gcgggaatgc
    33601 cgttgatgcc gccgtggctg tgggctacgc gctggcggta acgcatccgc aggcagggaa
    33661 tctgggcggt ggtggtttta tgctgatccg ctcgaaaaat ggcaatacca ccgctatcga
    33721 tttccgcgaa atggctcccg ccaaagcgac ccgcgatatg ttccttgatg atcagggtaa
    33781 cccggacagc aaaaaatcac tcacttcgca tctggcttct ggtacgcccg gtacggtagc
    33841 aggtttctcg ctggcgttgg ataaatacgg caccatgccg ttgaacaaag tggtgcaacc
    33901 ggcgtttaaa ctggcacgcg atggttttat cgttaacgac gcgttggctg acgatctcaa
    33961 aacctacggt agcgaagtgt tgccgaatca cgaaaacagc aaggctatct tctggaaaga
    34021 gggcgcgccg ctgaaaaagg gcgacaagct ggtgcaggcg aacctggcaa agagcctgga
    34081 gatgattgcc gaaaacggcc cggacgaatt ctacaaaggc acgatagcgg aacagattgc
    34141 ccaggagatg cagaaaaacg gtggcttgat cactaaagaa gatttagcgg cgtataaagc
    34201 ggtcgaacgt acaccgatta gcggcgaata tcgcgggtat caggtttact cgatgccacc
    34261 gccatcttcc ggtgggattc atatcgtgca gatcctcaat atcctggaaa acttcgatat
    34321 gcagaaatac ggctttggca gcgccgatgc gatgcaaatc atggcagaag cggagaaata
    34381 cgcctacgcc gaccgctcgg aatatcttgg cgatccggat tttgtcaaag tcccgtggca
    34441 ggcgctgacc aataaagcct atgccaaatc tattgccgat caaatcgata tcaataaagc
    34501 gaagccatcc agcgaaattc gccctggcaa gcttgcgcct tatgagagta atcaaactac
    34561 ccattactca gtggtggata aagatggtaa cgcggtggcg gtgacctata cgctgaacac
    34621 caccttcggt acgggcattg tcgcgggcga gagcggtatt ctgcttaata accaaatgga
    34681 tgatttctcc gccaaaccgg gcgtgccgaa cgtttacggg ctggtgggtg gtgacgctaa
    34741 cgcggtcggg ccgaataaac gtccgctgtc gtcgatgtca ccaaccattg tggtgaaaga
    34801 cggtaaaacc tggctggtca ccggaagccc aggcggtagc cggatcatca ctaccgtgct
    34861 gcaaatggtg gtgaatagca tcgattatgg tatgaatgtc gccgaagcga ccaatgcacc
    34921 gcgtttccac catcagtggt tgccggatga gctgcgtgtc gagaaaggat ttagcccgga
    34981 tacgctcaag ctgctggaag caaaaggtca gaaagtggcg ctgaaagagg cgatgggcag
    35041 tacacaaagc attatggttg ggcctgacgg tgagttgtac ggcgcatccg acccgcgctc
    35101 agtagatgat ttaacggcgg ggtattaagg ttagcggccc tcttcgtggg aagagggccg
    35161 tattgtcagg gcaagccgaa ggtagccttt tttatttcat aatcctgtag atattcttcc
    35221 agcggctttg ccgttccaag ggaatgaatt tcaccactgt ctttatcaat aataaaaggt
    35281 gcgttaccgg ctaagcgcgc ggcctcatca ccagattcaa gaaattcttg tgattcgaaa
    35341 cagaaatacc agccctggat aaaacgtcca tgcagagtga tgacgacagg gagatctgca
    35401 tcatcaaggt aatggttcgc tttcgcgaat gcgtcgtgat aa
//
LOCUS       DAZKGF010000026        35032 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZKGF010000026 DAZKGF010000000
VERSION     DAZKGF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.026"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..690
                     /locus_tag="ACHOQV_001300"
     CDS             <1..690
                     /locus_tag="ACHOQV_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFP9629698.1"
                     /translation="NVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDAV
                     RRTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKASASLAALWQVVNQIG
                     GKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLTL
                     LFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMAK
                     RIARLAEQSTD"
     gene            789..1802
                     /gene="ybjS"
                     /locus_tag="ACHOQV_001301"
     CDS             789..1802
                     /gene="ybjS"
                     /locus_tag="ACHOQV_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9629699.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1799..2629)
                     /gene="amiD"
                     /locus_tag="ACHOQV_001302"
     CDS             complement(1799..2629)
                     /gene="amiD"
                     /locus_tag="ACHOQV_001302"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFP9629700.1"
                     /translation="MRRVFWLVAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTLVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2626..2949)
                     /gene="ybjQ"
                     /locus_tag="ACHOQV_001303"
     CDS             complement(2626..2949)
                     /gene="ybjQ"
                     /locus_tag="ACHOQV_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP9629701.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGDQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            3075..3590
                     /gene="ybjP"
                     /locus_tag="ACHOQV_001304"
     CDS             3075..3590
                     /gene="ybjP"
                     /locus_tag="ACHOQV_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9629702.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRGNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3808..4536
                     /gene="artP"
                     /locus_tag="ACHOQV_001305"
     CDS             3808..4536
                     /gene="artP"
                     /locus_tag="ACHOQV_001305"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFP9629703.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            4554..5285
                     /gene="artI"
                     /locus_tag="ACHOQV_001306"
     CDS             4554..5285
                     /gene="artI"
                     /locus_tag="ACHOQV_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFP9629704.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            5292..6008
                     /gene="artQ"
                     /locus_tag="ACHOQV_001307"
     CDS             5292..6008
                     /gene="artQ"
                     /locus_tag="ACHOQV_001307"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFP9629705.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTVLRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            6008..6676
                     /gene="artM"
                     /locus_tag="ACHOQV_001308"
     CDS             6008..6676
                     /gene="artM"
                     /locus_tag="ACHOQV_001308"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFP9629706.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            6902..7633
                     /gene="artJ"
                     /locus_tag="ACHOQV_001309"
     CDS             6902..7633
                     /gene="artJ"
                     /locus_tag="ACHOQV_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFP9629707.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(7662..8789)
                     /gene="rlmC"
                     /locus_tag="ACHOQV_001310"
     CDS             complement(7662..8789)
                     /gene="rlmC"
                     /locus_tag="ACHOQV_001310"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFP9629708.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLSVKHMWDLFCGVGGFGLHCAMPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYCIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(8830..9318)
                     /gene="ybjO"
                     /locus_tag="ACHOQV_001311"
     CDS             complement(8830..9318)
                     /gene="ybjO"
                     /locus_tag="ACHOQV_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFP9629709.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(9378..10223)
                     /gene="potI"
                     /locus_tag="ACHOQV_001312"
     CDS             complement(9378..10223)
                     /gene="potI"
                     /locus_tag="ACHOQV_001312"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFP9629710.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDNAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(10220..11173)
                     /gene="potH"
                     /locus_tag="ACHOQV_001313"
     CDS             complement(10220..11173)
                     /gene="potH"
                     /locus_tag="ACHOQV_001313"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFP9629711.1"
                     /translation="MSTLEPAAQPKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(11183..12316)
                     /gene="potG"
                     /locus_tag="ACHOQV_001314"
     CDS             complement(11183..12316)
                     /gene="potG"
                     /locus_tag="ACHOQV_001314"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFP9629712.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPADGFNFAVGEVMHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRYRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(12411..13523)
                     /gene="potF"
                     /locus_tag="ACHOQV_001315"
     CDS             complement(12411..13523)
                     /gene="potF"
                     /locus_tag="ACHOQV_001315"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFP9629713.1"
                     /translation="MTALNKKWLSGLVTGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(13874..14350)
                     /gene="ybjN"
                     /locus_tag="ACHOQV_001316"
     CDS             complement(13874..14350)
                     /gene="ybjN"
                     /locus_tag="ACHOQV_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFP9629714.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(14438..15340)
                     /gene="rimK"
                     /locus_tag="ACHOQV_001317"
     CDS             complement(14438..15340)
                     /gene="rimK"
                     /locus_tag="ACHOQV_001317"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFP9629715.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(15401..16123)
                     /gene="nfsA"
                     /locus_tag="ACHOQV_001318"
     CDS             complement(15401..16123)
                     /gene="nfsA"
                     /locus_tag="ACHOQV_001318"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFP9629716.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALRDELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENNYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(16107..16394)
                     /gene="ybjC"
                     /locus_tag="ACHOQV_001319"
     CDS             complement(16107..16394)
                     /gene="ybjC"
                     /locus_tag="ACHOQV_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFP9629717.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSGSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            16554..16811
                     /gene="grxA"
                     /locus_tag="ACHOQV_001320"
     CDS             16554..16811
                     /gene="grxA"
                     /locus_tag="ACHOQV_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFP9629718.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(16841..17218)
                     /gene="ybjM"
                     /locus_tag="ACHOQV_001321"
     CDS             complement(16841..17218)
                     /gene="ybjM"
                     /locus_tag="ACHOQV_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFP9629719.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            17488..19173
                     /gene="ybjL"
                     /locus_tag="ACHOQV_001322"
     CDS             17488..19173
                     /gene="ybjL"
                     /locus_tag="ACHOQV_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFP9629720.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(19349..19885)
                     /gene="rcdA"
                     /locus_tag="ACHOQV_001323"
     CDS             complement(19349..19885)
                     /gene="rcdA"
                     /locus_tag="ACHOQV_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFP9629721.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            19969..21177
                     /gene="ybjJ"
                     /locus_tag="ACHOQV_001324"
     CDS             19969..21177
                     /gene="ybjJ"
                     /locus_tag="ACHOQV_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9629722.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            21177..21992
                     /gene="ybjI"
                     /locus_tag="ACHOQV_001325"
     CDS             21177..21992
                     /gene="ybjI"
                     /locus_tag="ACHOQV_001325"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFP9629723.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFSTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            22077..22361
                     /gene="ybjH"
                     /locus_tag="ACHOQV_001326"
     CDS             22077..22361
                     /gene="ybjH"
                     /locus_tag="ACHOQV_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFP9629724.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(22402..23634)
                     /gene="mdfA"
                     /locus_tag="ACHOQV_001327"
     CDS             complement(22402..23634)
                     /gene="mdfA"
                     /locus_tag="ACHOQV_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFP9629725.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHVWLNGGNGLFNLFNLVNGILWL
                     LLMVIFLKDKQMGNSHEG"
     gene            23919..24515
                     /gene="ybjG"
                     /locus_tag="ACHOQV_001328"
     CDS             23919..24515
                     /gene="ybjG"
                     /locus_tag="ACHOQV_001328"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9629726.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWSMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            24573..25331
                     /gene="deoR"
                     /locus_tag="ACHOQV_001329"
     CDS             24573..25331
                     /gene="deoR"
                     /locus_tag="ACHOQV_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFP9629727.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(25378..26580)
                     /gene="dacC"
                     /locus_tag="ACHOQV_001330"
     CDS             complement(25378..26580)
                     /gene="dacC"
                     /locus_tag="ACHOQV_001330"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP9629728.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            26827..27453
                     /gene="gstB"
                     /locus_tag="ACHOQV_001331"
     CDS             26827..27453
                     /gene="gstB"
                     /locus_tag="ACHOQV_001331"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFP9629729.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(27450..28565)
                     /gene="yliI"
                     /locus_tag="ACHOQV_001332"
     CDS             complement(27450..28565)
                     /gene="yliI"
                     /locus_tag="ACHOQV_001332"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFP9629730.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESDARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(28676..29059)
                     /gene="bssR"
                     /locus_tag="ACHOQV_001333"
     CDS             complement(28676..29059)
                     /gene="bssR"
                     /locus_tag="ACHOQV_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFP9629731.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            29272..30597
                     /gene="rimO"
                     /locus_tag="ACHOQV_001334"
     CDS             29272..30597
                     /gene="rimO"
                     /locus_tag="ACHOQV_001334"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFP9629732.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            30823..31209
                     /locus_tag="ACHOQV_001335"
     CDS             30823..31209
                     /locus_tag="ACHOQV_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9629733.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVIQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            31199..31528
                     /locus_tag="ACHOQV_001336"
     CDS             31199..31528
                     /locus_tag="ACHOQV_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9629734.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(31598..32926)
                     /gene="dgcI"
                     /locus_tag="ACHOQV_001337"
     CDS             complement(31598..32926)
                     /gene="dgcI"
                     /locus_tag="ACHOQV_001337"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9629735.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQLPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(32934..35032)
                     /locus_tag="ACHOQV_001338"
                     /pseudo
     CDS             complement(32934..35032)
                     /locus_tag="ACHOQV_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT         8865 a         9034 c         8499 g         8634 t
ORIGIN      
        1 aatgtgatta cttccgtacc gcccaccacc gccagggcgt tgattcaggg gctgaaacat
       61 gatctgctgg cggatgacac cgcgctacgt gcactcatcc cacaacggct gatcgctttc
      121 gatgacgcgg tacgtcgcac cctgaaagaa gaagaaaagc tggtcaactc cagcgactgg
      181 ggctacgacg ctcaggcctt tgcccgctgg cgaccggagt acggttattt cgccaaacag
      241 gcggggttta ccgttaaagc gtccgccagc cttgcggctt tatggcaggt ggtgaatcaa
      301 atcggcggta aagagcgtta tttctttggc aatattttgt ggcagacacg ggcgttgatg
      361 gaccgcgcga tcggtcataa gctggcgaaa ggccgcccgg agcgtgaata tttgcagacc
      421 ggcgatgcgg tggatagctg gaaagtgatt gtcgttgaac cggaaaaaca acttacgctg
      481 ttatttggca tgaaagcgcc ggggctggga cgactgtgtt ttaccctgga agataaaggt
      541 gactatcgta ctatcgatgt tcgcgcattc tggcatccgc acggtatgcc ggggctgttt
      601 tactggttat tgatgatccc cgcgcatctg tttatttttc gcggaatggc aaaacgaatc
      661 gccagactgg cagaacaaag cacagattaa tcgacaaaac ctgcgttatt ctttcatgtt
      721 tcccattgca tcatggggga aatcacggaa gaatgcgcac gttattttct caacaacagt
      781 ggattgatat gaaggtactg gttaccggcg ccaccagcgg cttaggtcga aacgcggtag
      841 agtttttatg ccagaaaggc atcagcgtgc gagcgaccgg tcgcaacgag gcaatgggta
      901 aattgctgga aaaaatgggc gcagagtttg ttccggcgga tctgaccgag ctggtctcat
      961 cacaagctaa agtgatgctc gcgggcattg atacgctgtg gcactgctcc agctttacct
     1021 cgccctgggg gacacaacag gctttcgatc tggctaacgt tcgcgccact cgccgcctgg
     1081 gtgaatgggc tgtcgcctgg ggtgtgcgta actttattca tatctcttca ccctccctgt
     1141 acttcgatta tcaccaccat cgcgatatta aagaagattt tcgccctcac cgcttcgcca
     1201 acgagtttgc ccgtagcaaa gcagccagcg aagaggtgat caatatgctt tcgcaggcga
     1261 atccacaaac gcgctttact attctgcgcc cacaaagtct gttcggaccg cacgataaag
     1321 tctttattcc ccgtctggcg catatgatgc accactacgg cagcattctg ttaccgcatg
     1381 gcggcagtgc gctggtcgat atgacctact atgaaaatgc cgtgcacgcc atgtggctgg
     1441 caagccagga agcctgcgat aagctacctt ccgggcgtgt gtacaacatc accaacggcg
     1501 agcatcgcac actgcgcagc atcgtgcaga agctgatcga cgagttgaat attgactgtc
     1561 gtattcgttc cgtcccctac ccgatgctgg atatgatcgc ccgcagcatg gagcgtttag
     1621 gccgcaagtc agcaaaagag ccgccgctga cccactacgg cgtctccaag ttgaattttg
     1681 actttacgct ggatattacg cgcgcgcagg aagagttagg ttatcagccg gtcatcaccc
     1741 tggatgaagg tatcgagaaa actgccgcct ggctacgcga ccacggaaaa ctgccgcgct
     1801 aatcctgccc gtatttctcc agcaacgctt cggcaatcgc ctgagtttct gcatccgctt
     1861 cgccgttgta taacgtcggg cggaaatgca tctggaacgc cataatcacg cgccgctgct
     1921 cgcgcggtgt catatcaggt ttaacgtcat aaccgtagcg cgccaaaagc tccagtaatg
     1981 atgcggtatc taccagtgtg tgcggcgcgc gcccggcaag gtaaaagttc acccgctgcg
     2041 catccggcca ggcaccaatc ccctgctgcg ccagttgttg ccaggggaat aacggccccg
     2101 gatcgtcttt gcgctgcggc gcaatatccg catgcgccac tacgttttcc ggcttgatgt
     2161 gataacgggc aataatatct ttcgccagcg gaataagcgc ctgaatctgt gcctgttcaa
     2221 acggggcaaa atatttcact ccggatgatt tttgccagcc acggttttcc agctcaatgc
     2281 caatagaggt gtcgttaaga cgcgttgccc cgcgccaggc gctaatcccc gcatgccagg
     2341 ccagttcctg ttccggcacc agttgccaga tgcgcggttt accgttgtat cgcggtggta
     2401 ccgcagggac cagataatgc gagctgacct gcttatcggt cagtgtcgcc agcgagctat
     2461 caaaatcatc tgcggtgtag tggataacca gcactttaat gcgcggatac gccgcctgcg
     2521 cctggcgtcg ggtatcaagc tgatatccct ctttctcgac aatgcctttt tcgcctgcac
     2581 accctgccag taacagagcg acagcgacca gccaaaagac tcttctcatc gacgcgtttt
     2641 caccgccgta ccgctgacgc taaccatcag catactgcca ttttgcccga ccgtttcgta
     2701 gtcgatatca ataccgacta cggcatcagc ccccagcgcc cgggcctgat cgccgagctc
     2761 ctcaaaggcg atctcccgcg ctttacgcaa ttctttttca taagccccgg agcgtccgcc
     2821 aacgatatcg cggataccgg caaagaaatc acggaaaata ttggcaccta aaatcgcttc
     2881 gccggtcacc acaccgcaat attcgacgat ggtctggcct tccagagttg gggttgtaga
     2941 aaattgcatg atcgatctcc ttgtttgcta agccttcgat cttcaaagca ttatcggact
     3001 gatacgctat tattgaaagg atatcattat tatcgtagaa cgctttcaag gtgttcgctt
     3061 ataaggaaat catcatgcgc tatagcaaat tgaccatgct tatcccttgc gcactgctac
     3121 tcagtgcctg taccaccgtc acgccagctt ataaagataa cggcccccgc accggttcct
     3181 gtgtccaggg ggggcctgat agcgtggctc aacagttcta tgactaccgc attcaacatc
     3241 gcggcaatga cattaccgct ctgcgcccct atttgagtga caaactggca accctactta
     3301 gcgatgccag ccgcgataat agtcatcgtg aattgttaag cagcgatccg ttctccagtc
     3361 gaaccacctt gccagacagc gcccacgttg ccagtgcatc gactatccct aatcgtgatg
     3421 cccgtaacat tccattgcgc gtggatctca agcagggtga ccagggctgg caggatgaag
     3481 tgttaatgat tcaggaaggc cagtgttggg tcatcgacga tgtgcgctac ttaggcggta
     3541 gcgttcatgc cacagcgggt acgctccgtc agtccatcga gaatcgctaa cgtcttgatc
     3601 aaccaataaa ccccgtaaat tgtacggcat ttcgtgagga atgccgacat ttatgctcgc
     3661 cgaccaccgc ccccgttatt ttgtgctatg tttattgaat aatgcgcttt gcttttaact
     3721 tttaaagcag aaatattgca taattattct gtcaaaggta ctatcttcga cctcaatagc
     3781 tatcagactg ccagtatacg agtgtcaatg agtattcaat taaacggcat taattgcttc
     3841 tacggcgcgc atcaggcgct gttcgatatc acgctggatt gcccacaggg cgaaacgctg
     3901 gtgttacttg gccccagcgg cgcgggtaaa agctcgctgc tgcgtgtact caatctgctt
     3961 gagatgccgc gctccggtac gctcaacatt gcaggcaacc atttcgattt caccaaaacc
     4021 ccctctgaca aagcgattcg cgatttgcgt cgtaacgttg gcatggtgtt tcagcaatac
     4081 aacctgtggc cgcatctgac cgtgcagcaa aacctgattg aagcgccctg tcgtgtactg
     4141 gggttgagta aagatcaggc gctggcccgt gcagaaaaac tgctggaacg tctgcgtctc
     4201 aaaccttata gcgatcgtta cccgctgcat ctttctggag gtcagcagca gcgtgttgct
     4261 attgcccgtg cgttgatgat ggaaccgcag gtactgctgt tcgatgaacc gaccgccgca
     4321 ctggaccccg aaattacggc acaaatcgtc agcatcattc gtgagctggc agaaacgaat
     4381 attacccagg tgatcgtcac ccacgaagtt gaagtggcgc gtaaaaccgc cagccgagtg
     4441 gtgtatatgg aaaacggtca tatcgtagaa caaggcgacg cgagctgctt taccgagccg
     4501 caaaccgaag catttaaaaa ctatctctct cactaagatt cgggataaca acaatgaaaa
     4561 aagttctgat tgccgcgtta attgcaggtt ttagtctttc cgccacagca gccgaaacca
     4621 ttcgttttgc caccgaagcc tcctatcctc cgtttgaatc gattgatgca aacaaccaga
     4681 tcgttggttt tgatgtcgac ctggcgcagg cattgtgtaa agagattgat gcaacctgca
     4741 ctttctctaa ccaggcgttt gacagcctga tcccaagcct gaaattccgt cgcgtagaag
     4801 cagtgatggc gggcatggat atcactccgg agcgtgaaaa gcaggtgctg tttaccaccc
     4861 cgtactatga caactctgcc ctgtttgtgg gtcagcaagg caaatacacc agtgttgatc
     4921 agctgaaagg caaaaaagtc ggcgttcaga acgggacgac acaccagaag ttcattatgg
     4981 ataagcatcc ggaaatcact accgtgccat atgacagtta ccagaacgca aaactggatc
     5041 tgcaaaacgg tcgtatcgac agcgtatttg gtgacaccgc agtggtaacc gaatggctga
     5101 aagataaccc gaaactggcg gcagtgggcg ataaagtgac cgataaagat tacttcggta
     5161 ctggcctcgg catcgcagta cgccagggca acactgaact gcagcagaaa ctcaacactg
     5221 cgctggaaaa agtgaagaaa gatggcactt acgaaaccat ctacaacaaa tggttccaga
     5281 agtaattcct gatgaatgaa ttttttcctt tagcaagcgc cgccgggatg accgtcggcc
     5341 ttgccgtttg tgcattgatt gtcgggctgg cgctggcgat gttctttgcg gtatgggagt
     5401 ctgcaaaatg gcgtcctgtc gcgtgggcag gttcagcgtt ggtgacggtt ctgcgcggcc
     5461 tgccagaaat tctggtggtg ctgtttatct attttggctc ctcgcagctg ctgctgacgc
     5521 tttcggatgg cttcactatc aatcttgggt tcgtgcagat cccggtgcag atggacattg
     5581 agaacttcga cgttagcccg ttcctttgtg gtgtcatcgc tctgtcactg ctgtatgccg
     5641 cctatgcctc gcaaacgctg cgcggcgcgt tgaaagcggt gccggtgggc cagtgggaat
     5701 ctggtcaggc gctggggctg tcgaaatcgg ctatcttttt ccgtctggtg atgccgcaga
     5761 tgtggcgtca tgcgctgccg ggtctcggta accagtggct ggtgctgctg aaagataccg
     5821 cgctggtcag tttgattagt gtgaatgatt taatgctaca aaccaaaagc atcgctaccc
     5881 gtacccagga accgtttacc tggtacattg tggcggcggc gatttacctg gtgatcaccc
     5941 tgctcagtca gtacattctc aaacgcattg acctgcgcgc gacacgtttt gagcggaggc
     6001 ccagctaatg tttgagtatt tacccgaact gatgaaaggg ttgcacacca gcctgacgct
     6061 aaccgttgcc tcgctgattg tggcactgat tctggcattg atttttacca tcatcctgac
     6121 gctgaaaacg ccggtgctgg tgtggctggt gcggggctat atcacgctgt ttaccggtac
     6181 gccgctgctg gtgcagatct tcctgattta ttacggaccg ggccagttcc cgacgttgca
     6241 ggagtatccg gcactgtggc atttgttgtc agaaccgtgg ctgtgtgcgc tgattgcgtt
     6301 gtcgctgaac agtgcggcgt ataccacgca gctgttttac ggtgcaattc gtgcgatccc
     6361 ggaaggtcag tggcagtcct gtagcgccct gggaatgagc aaaaaagata cgctggcgat
     6421 cctgctgccg tatgccttta aacgctcgct ctcttcttat tccaacgaag tagtgctggt
     6481 gttcaaaagt acctcgctgg cctacaccat tacgctgatg gaagtgatgg gatacagcca
     6541 gttgttgtac ggacgcacct acgatgtcat ggtgttcggc gcggcaggga ttatttatct
     6601 ggtcgttaac ggcctgctga cgctgatgat gcgtctgatc gagcgtaaag cgctggcgtt
     6661 tgagcgaagg aattaatttg gctgcgtgct gatgccggat gcggcgcgga cgccttatcc
     6721 ggcctacata gtgcataaat tcttataatg aagacggaca actcactaag ttgtccgtct
     6781 ttttttattt catttaaatt atttaatcat atttattgca tataaattca cttgatggca
     6841 ttgttatccc atgccgcaga cacggccaaa aatcataaga ttgacagacg ggagttccat
     6901 catgaaaaag ttagttcttg ccgcattact tgcttccttt actttcggtg cttctgccgc
     6961 agagaaaatc aattttggcg tttcagccac ctatccaccc tttgaatcta taggtgctaa
     7021 taatgagatt gtcggctttg atatcgacct ggcaaaagcc ttgtgcaaac aaatgcaggc
     7081 agaatgtact tttactaatc acgcgttcga cagcctgatc ccgtcactga aattcagaaa
     7141 atatgacgcc gtaatctccg gtatggatat cactccagag cgtagcaaac aggtatcgtt
     7201 taccacgccc tactatgaaa actcagccgt cgtgattgcc aaaaaagata cctacaaaac
     7261 gtttgccgat ctgaaaggca aacgtattgg gatggaaaac ggtactacgc accagaaata
     7321 tattcaggat cagcacccgg aagtgaaaac tgtctcctat gacagttatc agaatgcctt
     7381 tatcgatctg aaaaatggcc gtattgatgg ggtatttggt gacacagcgg tggttaatga
     7441 atggctgaaa accaacccgc aactgggcgt tgcgactgaa aaagtgaccg atccgcaata
     7501 cttcggcacc ggcctgggta tcgctgtacg tccggataac aaagctctgc tggaaaaact
     7561 gaataacgcg ctggcagcaa ttaaagctga cggtacatat cagaaaatca atgaccagtg
     7621 gttcccgcag taagattcag aaagccgcac ctgcggcttt tttattgctt aaccagcagt
     7681 gtcagcactt cgtaatgcgc agtgtgcggg aacatatcaa aaagctgtac ccgttcgatg
     7741 cagtaacctg gcagttcgcg gatatctttc gccatggttt gggcgttaca gctggagtag
     7801 ataataaaac gcggtgccat cgttgagaga taatcacaca gcggtttacc aatgccgcgg
     7861 cgcggcgggt taaccagcac cagctccggc acctccccct gagcagtggc aaactgagtg
     7921 gagtccagcg cctgaaattg caaacgcgtt aagcccagtt cagcggctga ctgctttgca
     7981 caggcaatgg cctctggcgc aatttcgatc ccggttaact gcatgtcagg catcgcgcag
     8041 tgtaaaccaa agccccccac accgcagaac agatcccaca tatgtttaac cgacagctgt
     8101 cgtacccagt cgcgcgcggt ggcgtacaac tggctggcga ccgccggatt agtctggaag
     8161 aaactttgcg gacggatcca cagcggtacg tcattaaaac gctctgccag cgcctgttgt
     8221 tcagtcaggt aaatctccgt ctccccttcc ataatcgcca tatgtaccgg ctgaatattg
     8281 acggtaataa ctttcagctg cggtagttgt tcctgtaacc acggcagcgc cttacgcagt
     8341 tgcgccagct tggtatcaga acgcagtaca aagcgcagca tcatgccgcc atcgctctgg
     8401 ctttcagtca gcagaatgta tttcagttcg ccacgcttac gcgccacgtt gtagggcgtt
     8461 aaccccgctc gggcgataaa cggttttagc gccgcaaaaa cgggcgcaaa tgaggcagga
     8521 taaagcggac agtcacaaag gtcttccggc gtgccgtcgc gatgcagcat accgagcagt
     8581 ggtttttcaa cgctaccact caccaccatt ttggctttat tacgaaaccc ttgttccggg
     8641 cctgacaccg gcgcgcacca ttcctcaacc ggaaagtcgg cgagcagatt tttaagatcg
     8701 gcggttttag cggagagttg ctctggaatc ggctgcgtta tccactgaca ggaacgacag
     8761 cgacccgcgt cgtaaagtgc gcactgcata cattgacctt cacatcatca ggggcgacga
     8821 ttatacacat tattgcaact ggaagaaccg ccgactggtc gagggaacga acagcagcat
     8881 caggatgagc atatccggca gcttctgcag catcaggcta tggaagattt cacgttttga
     8941 ttcaccggga atgctgaaca gctccggata accatacccc agcgaagctg cccacaagta
     9001 acttgcggcc gtgatttgtg tcaacagata cagccagcgc gcccagcgac gccctttcac
     9061 cagtgaaaac gcacaccaga tctcaatgaa aaccagcacc agactgctca aaaagaccag
     9121 cgttaaactc caggtttgta cgctgcgatg aatgaactcg ccaataccgc gcacgcccag
     9181 cgtattgaaa atcatcagca cgtcgaggcc acggatcata ataatggcga gcgccgccac
     9241 ctgcaccagc gcaggcacat tcaggcgagc atgagatgaa gatgttttct taaaaaatcc
     9301 caacgtttcg tcttccatga aaacaatgcc gcgacatgcg cggcattatg tagccaggtt
     9361 ggcaaatttt agtgtcttca gccacgtctt gcacgctgga tgtcgcgtat ccgctgtttt
     9421 tctgcgcgcg ccatcagata ccaggcgata aatccgacaa ttccgaccgc gccgaggatc
     9481 aatgttgcca gggcgttgat ttccggattc acccccatcc gcacgctgga aaagaccagc
     9541 atcggtaaag tggtggctcc cggcccggaa acaaagctgg cgatcaccag atcatcaagc
     9601 gacaaagtaa aagccagtaa ccagccagaa atgatcgcgg gcatgatcat cggtagcgta
     9661 atgacaaaaa acactttcag cggcgtcgca ccgagatcca tcgcggcttc ttctatcgag
     9721 cgatccagtt cccgcagacg cgacgaaata acgactgcca cataagccgt acagaaagtg
     9781 acatgcgcca gccagatggt gagcataccg cggtccgcag gccagccaat agcatgagca
     9841 agcgcgacga ataacaacaa tagcgacaag cccgtgatga catctggcat caccagcggc
     9901 gcggtgatca taaaggcaaa accatttgat ccgcgaaacc taccaaagcg caccagcacg
     9961 accgccgcaa tcgtcccgag gatcgccgcc gccgtcgccg cacaggccgc aattgtcagg
    10021 cttaaaccaa ccgcactcat catcgcgtta tcgcgcaata actcaccata ccagcgtgtt
    10081 gaccagccgg cccacaccgt caccagtttc gagctgttga atgaatagat caccagcatc
    10141 agcattggcg cgtagagaaa ggtgaagccc agcagcaaaa tcacaatccg ccagggcgaa
    10201 cgaacaaccg gtaaattatt catccgtgtt ctcccacgct tttttgctgg tgtttgtgaa
    10261 accacattat cggcacaatt agcagcagca acatgatgat cgctaccgcc gaggccaccg
    10321 gccagtcgcg gttattgaaa aactcctgcc acagcacgcg cccgatcatg atgctgtccg
    10381 ggccaccgag cagttcaggg atcacaaact cgcccacagc cgggataaac accagcatcg
    10441 atccggcaat aatcccgcct ttggtcagcg gcacgatcac agtaaagaac gttttcagcg
    10501 gtcgtgcacc gagatccagt gccgcttcca ccagcgaata atcaatacga atcaaggcgg
    10561 tgtagatcgg cagcaccata aacggcacat aggcgtaaac aatgccgata taaaccgcca
    10621 ggttggtatg cagaatggtc aacggttgat cgataacccc cagccacagc agaaaattat
    10681 tcagcacacc gttgtttttt aatattccca tccaggcata aacgcggatc agaaacgagg
    10741 tccacgacgg caggatcacc agtagtaata agatattacg ggtcgaaggc ttactgtgcg
    10801 ccaccgccca cgccagcgga tagccgatca gtaaacagca aaatgtcgaa atcgcagcca
    10861 cctgtagcga ctggagataa gcatcgaaat agagcggatc gtcagtcagt tgcagaaaat
    10921 taccgagatt aagagtgatg gacagttgcc cgtcagccca ctccatcagt tcggtataag
    10981 gcggaatggc gcgcgccatc tccgccagac ttattttaaa gacgatcaga aatggcagca
    11041 gaaacagcag gatcaaccag atatacggca acgcaatgac cagtttgcgc ccatgcttca
    11101 tttgcagctg cgacaaccac agcttaaatc cgcccggcgg tttgggctgg gcagcaggtt
    11161 caagtgtact catctttgct ccttaaaccg tcagcaccac acagctgtcc acttcccagc
    11221 acaaacgcac ttcgtcgccc caggttggta accctttgcg atatcgatga gcgttttgta
    11281 gctgagcgct gatcatctgt ccgctcttga gacgaacgtg atacaccgaa agatcgccga
    11341 gataggcaat gtgcatcacc tcacctaccg caaagttaaa accgtcagcg ggaggttcct
    11401 cgcaaagcat gattttttcc gggcgcagcg ccacatgcac cggcacatta tcgaccaccg
    11461 aagcatctac atcgactttc agtggatgca ccagccccgg cgaataaagc accagaccat
    11521 cttcctgacg ctctttgagt acaccttcaa agacattaac cgagccaata aattcggcgc
    11581 tgtagcgagt ggtcggatgc tcgtagatct cttccggttc gccaatctgg acaaatttcc
    11641 ctcgattcat aatggcgata cgccccgcca tggtcatcgc ctcttcctga tcgtgggtga
    11701 ccatcacaca agtcacaccg acacgctcca gaatatccac cacttcaagc tgcatccggt
    11761 cacgcagctt tttatccagc gcgcccatag gctcatcgag cagtaatagt ttcgggcgct
    11821 tggcaaggct tcgggccagt gccacacgct gtcgctgacc gccggaaagc tgatgcggtt
    11881 tgcgtttggc gaactcctgc atatgcacca gcccgagcat ctcattgacc cggctggcaa
    11941 tttccgcttt cggtagtttg tcctgtttca gaccaaaagc gatgttctgt tccacggtca
    12001 tatgcggaaa cagcgcgtaa gactgaaaca tcatatttat ggggcgcagg taaggcggaa
    12061 cctgtgacaa atcgacgcca tcaagcatta tctgtccggc agaaggttgt tcgaaacctg
    12121 ccagcatacg cagcagcgtg gacttgccac agccggatgc gcccagcagc gcgaagattt
    12181 cgcctttata gatggtcagg ctgacatcat cgaccgcatg ctgaccatcg taagatttag
    12241 tcagattacg gatttctaat agcggcgtca gcaccttacg ggttttcgcc tgcgggcgag
    12301 ggatagcgtc attcacggct gctctccggc attgaatgtg tgcaagcgca ccataatggt
    12361 gcgaattgcc ggcgggcgtg gtgcgcccgt ccggcatcta cgactgcgga ttattttccg
    12421 ctcttcactt tggtccacgc gcgggtgcgc acacggtcga ttttcggatc ctgtactttc
    12481 agagtgaaca gcttcgcacg aacatccgca ggcgggtaaa tacccgggtt atcacggact
    12541 tccgcactca ccagcggcgt agctgcttta ttggcgttag catagaacac atggtcggaa
    12601 atatgcgcca ccacatccgg gcgcagcagg taattcagga actgataggc ttcgtcttta
    12661 tttttggcat ccgcaggcat ggcgaagaca tcaaagaacg ccatcgcccc ttctttcgga
    12721 atcgagaacg agacattaac gccattcttc gcttccttcg cgcggtttga cgcctgccag
    12781 acatcgcccg cccagccaat agcgacgcag atatcgccgt ttgccaggtc gttaatgtat
    12841 tgagatgaat ggaaataacg aatgttcggg cgcagcttta acagcagatc ggttgccggt
    12901 ccggtgtaat catctgcttt agtgctgttg ggatctttac cgagataatt cagcacggta
    12961 gcaaacactt cttccggtgc atcaaggaaa gagacgccgc agcttttcag tttttccaga
    13021 ttttcaggct tgaggatcaa gtcccagcta tcgacgggcg cgttttcgcc cagcaccgct
    13081 ttaactttat caacgttata gccaatcccg gtcgtcgccc acatataggg catagcaaat
    13141 ttattgtcgg gatcgtgttt ggcaactaac ttcagcagct ccggatcgag attcttccac
    13201 tccggcaatt tgcttttgtc cagcggctgg aaaactcccg cagtcaactg gcgttcaaga
    13261 aagcttgctg acggcaccac caggtcaaag ccggtgctac ccgccattaa tttgccttcc
    13321 agtacttcgt tagagtcgaa aacatcgtag acgactttaa taccggtttc tttttcaaaa
    13381 ttggccaccg tgtccggggc gatataatca gaccagttat aaatgtgaag tgttttttgt
    13441 tcagccgcga gcgtgccgac agagacggcc atcagagcac ccgtaaccag acccgatagc
    13501 cattttttat ttaaggcggt catatctctt tccttctgaa agttcgttaa caaacgaaca
    13561 aaagttgtca cactgaaaga tatgcaataa acgtgcatat tttttatcgg cgcggtgtaa
    13621 gaaaagagaa ctctctcccg gccagcactg ctcgctatat aaaagcatcg caagaataac
    13681 tttagccagg gtttgagact atagctcgga ttaaaaaacg gtatttaaca aatttttatg
    13741 cgtatttgct ctatgtgata aagccaaatc ggcacaagag aagtgaaata atctttaaga
    13801 aaaggttaag aatagcagat aattttgcag cacgcagtca ctgcggcagc gactgcgtac
    13861 tcgaaaggag ttatcagtgg aggaaatagt tttcggtaac gttgttttgc ccttcatcgt
    13921 cagtcggtaa cagcagttga tgggcattag cttcaagaat gaccatcgaa atctgctctt
    13981 cgctctgacg cacgaaccat gcaaactgct cataggttac gccctgcata acggataaag
    14041 actggcaaac caccagcttt ggcagattat catcctgcat atcaagaaat gctttcacgg
    14101 tcagcgaact ggcattgatg gcagataaat ccgccgccag cggcaatacg gctgaaggtc
    14161 ggacttccgc catggcagaa aacaggatcg tgttatcgat cagatcgatt ttggcatcaa
    14221 agacaccgtc gaaattctgc atatggggca gatgcagagc ctgacagtta tcacattcaa
    14281 aaaaactcat ccccaggtca tcgagccatt gacgcagcgt atccagacca ggaacgacca
    14341 gcgatgtcat aattttgtac aacctctttc gataaaaaga ccggcacagc ttacgcaaaa
    14401 agcgcaggca aaaccatgat cagtaatgtg attgcgacta accacccgtt ttcaggcaat
    14461 attccgtcgt agcgtggcgt tcgatccagc ggatcatttt acccgcgatg tcgataccgg
    14521 tggttttttc tattccttcc agccccggcg acgcattcac ctccatcacc aatggcccgc
    14581 gattagcacg cagaatatcc acaccagcaa cgtccagcgc catcgttcgc gcggctttta
    14641 tcgcgatttc acgctcctgt ggtgtgatac tggcgacgct tgccgcgccg ccacgatgca
    14701 aattggaacg aaaatcgccc tctttcgccc gccgttcaat cgcagcgacc acttcatcgc
    14761 caacaaccag acagcggata tcgcaccctt gcgcctcttt gatatattcc tgcaccagaa
    14821 tatgcgcgtt cagaccgcgg aaagcgtcaa tcacgctttc cgccgcctgg cgcgtctccg
    14881 ccagcacgac accaattccc tgcgtgcctt caaccagctt gaccaccagc ggcgcaccac
    14941 cgaccatgtc gattaaatcg ctggtatcat ccggcgaatg cgcaatgccc gtgacaggca
    15001 ggtcgatgcc ctgacgcgcc agcagttgca tggaacgcaa tttgtcacgc gcccgggcaa
    15061 tggcgaccga ctcattgagc ggatagctcc ccagcatctc gaactggcgc agtgccgccg
    15121 tcccataaaa ggtaatggcg gtaccaatac gcgggatcac agcatcaaaa tggggtaact
    15181 tgcggccttt gtagtgaata gaagacgccg caggatttat gttcatgtag caagaaagcg
    15241 gatcaagaat ttcaaccagg tgaccgcgct gtatagcggc ttcacgcagc cgcttacacg
    15301 aatagagcgt tccatcccgg gacaatatgg caattttcac cctgcacctc tctgtcagac
    15361 ctggtcaaaa gcccgcgtat catacatcgg cgtggcggtt ttagcgcgtc gcccaaccct
    15421 gtttgtgcaa ataatccagt ataaatgggc ggctttcttt aatgattgtt cggcggatat
    15481 gatcgctcca ggtatcccgg cgattattgc tgccacgggt gaggtaatat tccgccagtt
    15541 gttcgtcata ctgcgccagt gcgtctttat ccagcggttg atagttgttt tcatgcacca
    15601 aaatggaggc cggtaaacgc ggcttaagat ccggattatc cgcaggccag ccaaggcaca
    15661 gcccaaacag cggcagaaca tgctgcggta atttcagcag ctccgtcacc gcttcaatat
    15721 tattgcgcag gccgccgata tataccccgc ccaatcccag cgattccgct gcggttaatg
    15781 cattctgcgc catcattgcc gtatcaacga caccgagcaa cagttgttcc gccaggccaa
    15841 gttgtgcatc cggacagatc tgtaaatggc ggttaaagtc ggcacagaac acccagaact
    15901 ccgccgcttg cgctacgtgt ttttgcccgc cggtcagcgt caccagttca tcacgtaacg
    15961 ctttgtcggt aatgcgaata atgctactgc actgcaaaaa actggaactg gacgtcgcac
    16021 gggcgctgtt aataatcgcc tcacgctgcg cttcggaaat gggttcatca gtgaaatggc
    16081 gaatggagcg atggccacaa ataagttcaa tggttggcgt cattatcttt ttctctttct
    16141 gaacgtgaat attgcggtgg acggttcatc agctgtgggg caagacgttt tgccacctga
    16201 agaataacca ccaccgcagc gggaagcatg agcaaaacac cgagaaaaat catcagaatc
    16261 tgcacttctg gccgagaaaa tggctcaggc agcgacaggg agccgcttac cgacagcagc
    16321 gccaccgcca gcaacatcat tccgataaat tccagtatca acacgctttt aggcaattta
    16381 ccgatcgcgc gcatacgctt ccctctgcaa agtgagcctt cagtctaaaa cttttcactg
    16441 tattgtgttt aacagttata gcttttagca attaatgcaa caggttaaac ctactttcag
    16501 cgaatacatt ttagcgtgat cattacaggt ataaatctat gaggagagaa ataatgcaaa
    16561 ccgttatttt tggtcgtccg ggttgccctt actgtgtgcg tgcaaaagat ctggctgaga
    16621 aattgagcaa tgaacgcgat gatttccagt atcagtatgt agatattcgt gcggaaggga
    16681 tcactaaaga agatctacaa caaaaggcag gaaaacccgt agaaaccgtg ccgcagattt
    16741 ttgtcgatca gcaacatatc ggcggctata ccgattttgc tgcatgggtg aaagagaatc
    16801 tggacgcctg atcgtctgac aagccctcgc gttgagggct ttattgattt tttctgtgct
    16861 gtggtttaaa caaactactg ataaataaga aacacaatgc ccccagcgca caccagaaca
    16921 ccgcacttaa taaccatgcc agctcttgcc agaatgagcg cgtcggtgaa aaaaacagcc
    16981 gcataatgag catcgaacag ggtgccgcca gcattgcgcc aaacagaggt ttcaggactt
    17041 ctctacgatg tgaaaagaag ctggcaactg caccaggaag aatgaaaaat agcaaaccga
    17101 tttcaggatg cccggcagcc cgaaaagcgc ctttcatgtg cgtcgccaga aaaagacaca
    17161 ccacaatgaa gaggacaaaa cagcagattg cccccgccca acgttgttta tgtttcactc
    17221 gttcctcctg acactgcgtc tatcgaacac atttttcgcc agtgtggcgt tcagtaagat
    17281 aaagccgctt cgcattccat gctaatatag gccaacgcaa ttcatatagc cgttgatacc
    17341 taatgtgatt acactagtaa aatatattgt tactttacta tcgtttaggt gcgctgaatg
    17401 aatctgcgcc ctgaattctg gtaaaaaaca ttatcgtaaa ttaccatttc tttcaacagc
    17461 ttactagtaa acaagaagtt agcctccgtg aatataaacg tcgccgaatt gttaaatggg
    17521 aattacattc tgttattatt tgtggtcctc gcgcttgggc tatgtctcgg gaaattacga
    17581 cttggttcga tccaactggg taattccatt ggcgttttag tcgtatcgct gttattaggc
    17641 caacaacatt tcagcattaa caccgacgcg cttaatcttg gctttatgct gtttattttc
    17701 tgcgtcgggg tcgaagccgg accgaacttt ttttccattt tttttcgcga tgggaaaaat
    17761 tacctaatgt tagcactggt gatggttggc agtgcgctgg tcatcgcctt agggttaggt
    17821 aagctgtttg gctgggatat tggcctgacg gccggtatgt tagcaggctc tatgacgtca
    17881 acaccggttc tggtcggtgc tggcgataca ctgcgtcatt ccggcatgga aagcaggcag
    17941 ctctcattgg cactggataa tctgagcctc gggtatgcct taacctattt aatcggcctg
    18001 gtgagtttga ttgttggtgc gcgttacttg ccgaaattgc agcatcagga cttacagacc
    18061 agcgcccagc aaatcgcccg cgaacgtggc ctggacacag atgccaaccg taaggtttat
    18121 ttgccggtga tccgcgccta ccgcgtcggc ccggagctgg tagcctggac cgacggcaaa
    18181 aatctgcgtg aactgggtat ttatcgacaa actggctgtt acattgaacg tattcggcgt
    18241 aacgggattc tggcaaatcc agacggtgat gccgtactac aaatgggcga tgaaatagcg
    18301 ttggtaggct atcctgacgc ccacgcccga ctcgatccca gtttccgtaa tggcaaagaa
    18361 gttttcgatc gtgaccttct cgacatgcgt atcgtcactg aagaagtggt cgttaaaaac
    18421 cataacgctg ttggcaaacg cctggcacaa ctgaagttga ccgatcacgg ttgcttcctt
    18481 aaccgcgtca tacgtagcca gattgagatg ccgattgatg acaacgtcgt gcttaacaaa
    18541 ggtgacgttt tacaagtcag cggcgatgcc cgccgggtaa aaaccatcgc cgatcgcatc
    18601 ggctttatct cgattcacag ccaggtcact gacttgctgg cattctgcgc cttctttgtt
    18661 attgggctga tgatcgggat gatcaccttc cagttcagca cattcagttt cggtatgggg
    18721 aacgctgccg ggttgttatt cgccggaatt atgctgggct ttatgcgtgc taaccacccg
    18781 accttcggtt acattccgca gggtgcatta agcatggtga aggagttcgg cttgatggtg
    18841 tttatggcag gcgttggtct gagcgctggt agcggtatta ataacggcct gggcgcgatt
    18901 ggcggtcaga tgttgattgc cggattgatt gtcagtctgg tgccagtggt tatctgcttc
    18961 ttgttcggtg cttatgtatt gcgaatgaac cgcgcactgt tgttcggcgc aatgatgggc
    19021 gcacgcacct gcgcgccggc aatggagatc atcagtgata cagctcgcag taatatcccg
    19081 gcactgggct atgcgggcac ttacgcaatc gccaacgtcc tgctgacgct ggcagggaca
    19141 atcatcgtca tggtatggcc aggattagga taaaactgaa gttgccctga aaatgaaatt
    19201 ttttttgcac aaccgcagaa cttttccgca gggcatcagt cttaattagt gccactgctt
    19261 ttctttgatg tccccatttt gtggagccca tcaaccccgc catttcggtt caaggttgat
    19321 gggttttttg ttgtctaaaa tttatctact accctgcaac cctctcaacc atcctcaaaa
    19381 tctcctcgcg cgatagcggc ttacggtcgg tgacaaaatg cagcgtcatc ccctcaataa
    19441 acgcatcaag cgcgcgggcg gttccgggtt caaaccattg ttcgagcgtt tgctgactgc
    19501 gctgcatcca gttttgcatt accgttttta atagcggttt tcggctagcc agcgcgtaga
    19561 gctggtacat cagctccatg ttatccggcg ttgcaacctg tgagctgtaa atcatatcgg
    19621 tgatagcctg gcatgcgccc ggagcatcac taacatcgct aaaaaatgcc tgatattgcc
    19681 gggacatgat ctcagtaaaa ctgctgaacg cctccagcaa caactcatca attcctgaaa
    19741 aatagtaggt catcgacccc aacggtaccc cggcaagggt agcaattttg cggtgcgtaa
    19801 cagcatgtat tccataaagt ttcaccgcct ccagcgtggc ctggataatt ttttctcgcc
    19861 gttgcggatc gttagcgcga cgcatgtcat ttcctctctc tgcaattgtg tacaaatgta
    19921 cacaaccttg ctaatgttgt cttccctctt cttattttga cgtgatctat gaccgtaaat
    19981 tcttcacgta atgcattgaa acgccgaacc tgggcgctgt ttatgttctt ctttttgcca
    20041 ggcctgttaa tggcgtcctg ggcaacccgt acgcctgcta tccgcgatat tctttctgtt
    20101 tcgatcgctg aaatgggcgg agtcctcttt ggtctgtcga tcggttcaat gagcggtatt
    20161 ctctgctcgg cgtggttagt gaaacgcttt gggacgcgta atgtcatcct ggtcacgatg
    20221 tcctgcgcat tgatcgggat gatgatacta agtctggcac tctggctgac atcgccccta
    20281 ctcttcgcgg ttggtctcgg cgtctttggg gcaagttttg gttctgcgga agtggcgata
    20341 aacgttgaag gtgctgccgt tgagcgagaa atgaataaga cggttttgcc gatgatgcac
    20401 ggtttttata gcctgggcac gctggcaggc gctggtgtcg ggatggcact aacggccttt
    20461 ggcgttccgg caacggtgca cattttattg gcggcgctgg taggcatcgc gcctatttat
    20521 atcgccattc aggcaatccc tgatggtacg ggcaaaaatg ctgccgatgg cacccagcat
    20581 ggtgaaaaag gcgtaccttt ttatcgcgat atccagttgc tgctgattgg tgttgtggtg
    20641 ctggcgatgg cctttgccga aggttctgcc aacgactggt tacccttatt aatggttgac
    20701 ggtcacggtt ttagccctac ttccggctcg ctgatttatg ccggttttac cctggggatg
    20761 accgttggac gctttaccgg cggttggttc attgaccgtt acagtcgcgt tgccgtggtt
    20821 cgggccagtg cactaatggg ggcgttgggt attgggctga ttatttttgt cgatagcgcc
    20881 tgggttgctg gggtgtctgt tgtactttgg ggactgggtg cctcgttggg cttcccgctg
    20941 accatttctg ccgccagcga taccggcccc gatgcaccaa cccgcgtcag cgtggtagct
    21001 acgaccggtt atctggcttt cctcgtcggg ccgccactgc tgggctatct cggcgaacat
    21061 tatggattac gtagtgcaat gctggttgta ctggcgctgg ttattctcgc ggctattgtc
    21121 gcgaaagccg tcgccaaacc cgataccaaa acgcagacgg cgatggagaa tagttgatga
    21181 gcattaaatt aattgcggta gacatggatg gtactttctt aagcgatcaa aaaacctata
    21241 accgtgagcg gtttatggct cagtatcagc aaatgaaagc acaaggcatt cgctttgtgg
    21301 tcgccagcgg gaatcaatat tatcagttga tctctttctt tccggaaatt gctaatgaaa
    21361 tagcctttgt ggctgaaaac ggcggctggg tagtgagcga aggcaaagat gtttttaatg
    21421 gcgagctgtc gaaggatgcg ttttctactg tcgtggaaca tttgctgacg cgcccggaag
    21481 tggaaattat tgcctgcgga aaaaatagcg cctatacact caaaaaatat gacgatgcca
    21541 tgaaaacggt ggcggaaatg tattatcacc gtctggaata cgtcgataac tttgacaact
    21601 tagaagatat cttctttaag tttggtctga atctttccga tgaactgatt ccacaagtac
    21661 aaaaagcatt acatgaggcc atcggcgata ttatggtgcc ggtccacacc ggcaacggca
    21721 gcatcgatct gattatcccc ggcgtacata aagccaatgg ccttcgccaa ctgcagaaat
    21781 tatggggaat agacgacagc gaagtggtgg tctttggcga tggcggtaac gatattgaga
    21841 tgctgcgtca ggcaggcttt agttttgcaa tggaaaatgc cggcagcgcg gtcgtcgcag
    21901 cggcaaaata ccgggcaggc tccaataacc gtgaaggcgt actggatgtg atcgataaag
    21961 tgcttaaaca cgaagcgcca tttaaccaat aaaacggcag cacgcccggc aacacaatac
    22021 ctgaatgaag gctgcataat tcgcggaaat taacccgctg cattatttca ggcgaaatga
    22081 ttatgaaaaa ttgtctactg ttgggcgcgc ttttaatggg ctttactggt gtggcgatgg
    22141 cgcaaagtgt caccgtcgat gtgccgagtg gatacaaagt ggttgtggtt cctgattcag
    22201 tcagcgttcc acaagcggtc agcgtcgcga ctgtgccgca aaccgtctat gtggctcctg
    22261 cgccagctcc ggcttaccgc ccgcatcctt atgttcgcca tcttgccagc gtaggtgaag
    22321 ggatggttat cgaacaccag attgacgacc atcaccatta attcgagaat gcctgatcgc
    22381 acaaagcaat caggcatttt tttacccttc gtgcgaattt cccatctgtt tatcttttaa
    22441 aaagataacc atcagcaaca gccacaaaat tccgttgaca aggttgaaga gattaaacag
    22501 tccgttgccc ccgttcagcc agacatgttt gctgatttca ataccgacgg taaagatcag
    22561 catttgcagc atccccatcg ccgcagaaac cgtaccttta ctcatatcgc tggcaaacag
    22621 ggttaatcgc accagtcccg cattcgccag accaataccg aaagcataaa tacttaaccc
    22681 ggcggtcatc cataaatacg cgtgcgatga gataaccgtt gccgcagcag cgaccaacag
    22741 accaatcata atcggccagc cgcccataat aatcagcgaa cgtacggtgc ggcgcgaggt
    22801 cagacgcgct aacagcaagt tacccgcaat taacgccccg aaaataggca cttgcagcaa
    22861 gccatattca tagctgctca actgctcacc ggtaataatg ataatcggcg actgggcgat
    22921 ccacgccaac aacggcaggc taacgaatcc cagcgccagc gcccccgcca caaagcggcc
    22981 gttcttcagc accagcttat agtcacgacc gagttctttc agcgacagtt tctcgcctat
    23041 acgcgtggcg gtttcaggca tggctcgttg cagaccgaaa aaggagatcg ctgccaatgc
    23101 ggcaaacaag acaaacatcc cttcccaggg cagcacatgg atccacgccg cgcccaccag
    23161 cggaccaagt agtggagcaa tcagcgccac gttcgccatt agcgcggtga tcttgataca
    23221 aaccgcctct tcgaaggatt cctgaattgc ggcgtatccc acagcgccaa tgaaacagag
    23281 gcttatgccc tgcaagaagc gcaacagggt gaattgttca atgttttgcg ccagtaatat
    23341 tgccagacag gtgacgataa accacaccac tcccgccagc atcaccggac ggcggccaat
    23401 acgatccgac aacggcccca ggagccattg taaaaacatc ccgcccgcca gatacgcagt
    23461 catcgaagta ggaacccaat caatgcccgc ctgatattgt tccaccacgg ccaacatacc
    23521 gggttgaatc atatcgttgc cgatataggt tgaaaattcg taaagcacca gacagagagg
    23581 gaaaagtaac gcctgacgtc caagcctggc accggaagct aatttatttt gcatgcaatt
    23641 tcttcgccaa taataatcgc gcaaagttta ataaaagcgc agctaacgag aaagcgaatt
    23701 ttgtagctga aaccacggtt aagcacattc ttacattatt gcgagtatag ctacgctttc
    23761 tttaagtttt atttaacctt tgcccgttac aatcacccac cgtaaacagg ccgcttgagg
    23821 gaaataagac gatgccgctt tacccagttt aacctgcact ttattctcaa cgacttgcct
    23881 gtattggctc ccttttaatc actttgcgtc gggaagttat gctggaaaat ttgaatctct
    23941 ctctattctc tcttattaac gcgacgccag actctgcgcc gtggatgatc tcgttggcga
    24001 tttttattgc aaaagatttg attaccgtgg tgccgttgct ggccgcggta ctttggctgt
    24061 gggggcttac agcacaacgg caactggtga taaaaatcgc tatcgcgctg gcggtcagcc
    24121 tgtttgtgtc ctggtcgatg gggcatcttt ttccgcacga ccgacccttt gtcgaaaata
    24181 tcggctataa cttcctgcat catgcggcgg atgactcatt cccgagcgat cacggtacgg
    24241 tgattttcac ctttgcactg gcatttttat gctggcatcg cctgtggtcc ggctcacttt
    24301 taatggtgct ggccgtcgtc attgcctggt cgcgcgttta tcttggcgtc cactggccgc
    24361 tggatatgct cggtggattg ctggcaggta tgattggctg ccttagcgcc cagattatct
    24421 ggcaagcgat ggggcataaa ctctatcaac gtctgcaatc gtggtatcgc ttctgttttg
    24481 cattaccgat ccgcaaaggc tgggtgcgtg actgatctct gctaaaaagt gtagtattga
    24541 gcggctcgct tcaataacta ttcagaggga ttatggaaac acgtcgcgaa gagcgtatcg
    24601 ggcagctgct gcaagaatta aaacgcagtg ataagttaca tcttaaagac gccgccgccc
    24661 tgcttggggt ttcggagatg acgattcgtc gcgatctgaa caaccacagt gcgcccgtcg
    24721 ttttgctcgg cggctatatt gttctggaac cacgcagcgc cagccattac ctgttaagcg
    24781 atcaaaaatc ccgcctggtg gaagaaaaac gccgggcggc aaaactggct gcgacgctgg
    24841 tagaacccga tcagaccctc ttttttgact gtggcaccac cacgccgtgg attattgaag
    24901 caattgataa tgaaatccct tttaccgccg tttgttattc gctaaatacc tttctggcgc
    24961 tgaaagagaa accccattgc cgcgcgtttc tttgcggtgg tgaatttcac gccagcaacg
    25021 ccattttcaa acccatcgat tttcagcaaa cgctgaataa tttttgcccg gatatcgctt
    25081 tttattccgc tgcgggcgtg catgtcagta aaggcgctac ctgctttaat cttgaagagt
    25141 tgccggtaaa acactgggcc atgtcgatgg cgcaaaagca tgtgctggtt gtcgaccaca
    25201 gtaaatttgg caaggtgcgt ccggcgcgca tgggtgacct gaaacgcttt gatattgttg
    25261 tgagcgattg ttgcccggaa gatgagtatg tgaagtacgc gcagacgcag cgtattaagt
    25321 tgatgtatta atgacgtata accggatgac gtttcgcgcc atccggttat cagaagatta
    25381 agagaaccag ctgccgaacc actgatggaa tttcatcatc acgaaatccc acatccgacc
    25441 aaagaatccg ccctcttcca cattttccat cacgatcagc ggacgctgct caatggattt
    25501 accgttaagc tggaaatcaa tggtgccgac aacctgacct tttttcagcg gtgcggtaag
    25561 ctgcggttcc gttaacgtat aactcgcctt caggtttttc agctgcccac gcggtatggt
    25621 cactgagccc gcttcgcctg ccccgagatt cacttcgctc ttatcaccaa accagacgcg
    25681 ctgagtcaca aatgtggcat caggtttaat tggcgtcacg gtttcaaaga aacggaagcc
    25741 ccaggtcaat aatttctcag actcattaaa acggatacgg tcagttttcg cccccagcac
    25801 tacggagatt aaacgcatat cgccctgggt agccgaagca accagattat atcccgcgcc
    25861 tgccgtggtt cctgtcttca tgccatcaac attcagattg ctgctccaca gcagacgatt
    25921 acggttaggc tgacgaattt tgttgaaggt gaactctttc tctttatgaa tggcgtactc
    25981 ttccggcaca tcgtggatca atgctttacc cagcaatgcc atatcgcgcg cggtactgaa
    26041 ctgccccggc gcatccagac cgtgcaccgt ctggaaggta gtgttggtca gacccagttt
    26101 tttggcataa ccattcatca gaccaataaa tgactcctgg ctcccggcaa cgtaatcagc
    26161 cagcgcaata caggcgtcat taccggactg gataatcaca cctttgttca agtctgccac
    26221 cgaaacctga tcgcccggtt tgaggaacat taccgatgaa ccacgcagtg ccggatttcc
    26281 cgtcgcccag gcatctttac cgaccgtcac catatcggtg agtttaatct tatcggcctt
    26341 aagcgcctgc ccaaccacat agctggtcat gattttagtc aggctcgcgg ggtccagttt
    26401 ttcatccgca ttgccttctg ccagcacttt accgctggcg taatccatta aaatccatgc
    26461 acgcgcatcc acgctcggcg cttcaacggt ttgttccgcc gcgaatgccg ttggggcaaa
    26521 aaggaataaa aatgcagaac ccgctgcaag accacgaagg agagaggagt attgcgtcat
    26581 aaatgccacc cgagtatcca ttcaaaaaaa atacgttata tcgccgtatg ataagttcgg
    26641 tgattaataa cgcacacaca aacttaaaga aaccgtaagt tggtaaagtt tttaaagttt
    26701 atgcaatatc ctgccgcttc gctgcttctg gtgtcatttt tttgccagct gttgcttaaa
    26761 agttaacttt acttcaccat cggagtcctg ttcagcctgt ggctgcctgt cggaaaaggg
    26821 gtgagtatga ttacgctgtg gggtcggaat aattcaacca acgtaaaaaa agtattgctg
    26881 acgctcgaag aactggaact accttatgag caaattctcg cgggccgtga gtttgggata
    26941 aatcacgatg ctgattttct ggcgatgaac cctaacgggc tggtgccgtt gttgcgtgac
    27001 gacgaaagtg atctcattct ttgggaatca aacgccattg tccgctatct ggcggcacag
    27061 tacgggcaaa aacgcctgtg gatcgactca ccggcacgtc gtgcggaagc cgaaaaatgg
    27121 atggactggg caaaccagac gctcagtaat gctcatcgcg ggatcctgat gggattagtc
    27181 agaacaccac cggaagagcg cgatcaggcc gccattgatg ccagttgcaa agagtgcgac
    27241 gccctgtttg ccctgctcga tgcggaactg gcaaaagtaa aatggttctc aggcgacgag
    27301 tttggtgtgg gcgatatcgc tatcgcaccg ttcatctaca atttgtttaa cgttggcctg
    27361 acctggacac cgcgtccaaa tctgcaacgc tggtatcagc aactcactga acgccccgca
    27421 gtgcgcaaag tggtgatgat tcccgttagc taattgcgtg ggctaacttt aagtaattcc
    27481 ccactggact cgtcggtgag aacgtataaa taaccgtcgg gtccagtgcg aacatcacga
    27541 attcgctgcc ctctgtccgt taaaatacgg ccatcttctg tcactttgtc gccgttgacg
    27601 ctcatcacaa tgacatcttt atctttcagc gcgccaataa ataatttttg ctgccactgg
    27661 gggaatttgt cgctgttata gaaggccatg ccgctcacgg cgggcgaatc tttccagtaa
    27721 aaaacaggtt gctcggtccc ggcgacgatc tcccctttcg cctccggtat cttaaagcct
    27781 gaatagttga ttccccaggt tgccagcggc cagccgtagt ttttgccttt ttgcgggata
    27841 ttaatttcat cgccaccgcg cgggccatgt tcattcagcc atagtgcatt actccacgga
    27901 ttcatcgcca ttccttgcgg attacgaatg ccataagacc agatctcggc gcgtgcatcg
    27961 gattccttta taaaaggatt atcatccggg atttctccca ggtcggtcag tcgcaccagt
    28021 ttgccctgta atttatccag atcctgcgcc gtcgggcgct gattgttttc gcccagagca
    28081 ataaaaagat aacctttacc gtcaaatacc atccgcccgc caaaatggtt gccggtagac
    28141 agttttggca tctgacgaaa gacggtgcgg aagtcggtca cttttgagag atcatcactt
    28201 aagcggccat aacccacagc agttccggct ttgccatcat cgccaacttc ggaataactt
    28261 aaccagatgc ggcgcgactg agcaaaatcg ggcgctaaaa ccacgtccag caggccgccc
    28321 tgcccgtgcg cccaaacgtc cggcactccg gaaagcggcg cagataatcc ttttcctgct
    28381 tgccagtgac gcaactcgcc gccgcgcaga gtgattaaca taccgtgatt gtcgggtaaa
    28441 aaggccagcg cccagggatg gtcgagtttg tcctgcagta cttcgacatt taccgttgca
    28501 ggagccgccc agagagcgga agaaagacaa ataaggggca caaggaaaaa ggattgtcga
    28561 tgcataacgc ctccggattg actctggctt aaagcgtagt cagtggagga gataacaaat
    28621 ttatttttac aaaaacttaa acatgaaggg ggagacgctt tctccccctt agttttcagg
    28681 ccttctcaag catggcgtgc ttctgcaggc tctggatact cagcgttaag ctcatcagac
    28741 aattttcaag cttatcggcg ttgacggtaa taacagtcgg gcaatcatgg tgcccactca
    28801 tcaaacatac tgcggctgtc gctaatgctt cttcagcatg atgaagagca ctccactctt
    28861 cctgatccag atgaagattc aaccgcagcg atttatcgtg cagttcgcga ttcagtttaa
    28921 aaaagttatc tcgtagatga ttgctttcgc tgacggacat gtatcctttt gccttcctca
    28981 gttgcacgta tgcggcgagg tcaacgcgtg cgtcgatcaa ccggttcagc agatcgattc
    29041 gctgtctatc aacgaacata cttcgatcct cctctttcca gccttgttgc aattaaatag
    29101 tatggctatt ttttgagcaa aatgctgtct aggatcaatt cttcacgccg aattaacaaa
    29161 atttacattt ccttcgcctc cgccagcgaa tgagattgcg atacaaattt tatccagaac
    29221 tctgtagaat ccctgccctg acctttactg taactgaata ttttttaagc tatgagcaaa
    29281 gtaactcccc agccgaaaat cggctttgtt tcccttggct gcccgaaaaa ccttgtcgat
    29341 tcagagcgta ttctcaccga actacgcact gaaggttatg acgtggtacc gagctatgac
    29401 gatgcggaca tggtgatcgt caacacctgc ggctttattg acagcgcggt acaagaatca
    29461 ctggaagcca ttggtgaagc gttgaatgaa aacggcaagg taattgtgac cggttgtctg
    29521 ggggcaaaag aagatcagat ccgcgaagtc cacccgaaag tgctggaaat caccgggcct
    29581 catagctatg agcaggttct ggagcacgtt catcactacg tgccaaaacc gaaacacaac
    29641 ccattcctga gcctggtgcc agaacaaggt gtgaaactga cgccgcgtca ttatgcctat
    29701 ctgaaaattt ctgaaggctg taatcaccgc tgcaccttct gcattattcc gtctatgcgc
    29761 ggcgacctgg tgagccgtcc gattggcgaa gtgttaagtg aagcgaaacg tctggttgat
    29821 gcgggcgtta aagagattct ggtgatctcg caggatactt ccgcctatgg cgtcgatgtt
    29881 aaacatcgta ctggcttcca caacggcgag ccggtaaaaa ccagcatggt cagcctgtgc
    29941 gaacagttat cgaaactggg gatctggaca cgtctgcact acgtttaccc ttatccgcat
    30001 gtggacgacg tcatcccact gatggcagaa ggcaaaatcc tgccctatct ggacattccg
    30061 ttgcagcacg ccagcccgcg cattctcaaa ctgatgaagc gtccgggttc tgtagatcgc
    30121 caactcgcgc gcatcaaaca gtggcgcgaa atctgcccgg aactgaccct acgctcaacc
    30181 tttattgtcg gcttccctgg cgagacggaa gaagatttcc agatgctact cgacttcctg
    30241 aaagaagcac gtctggatcg cgttggctgc tttaaataca gcccggttga aggtgcagac
    30301 gccaatgccc tgcctgacca ggttccggaa gaagtgaaag aagaacgctg gaaccgtttc
    30361 atgcagttgc agcagcagat ttccgccgag cgcctgcaag agaaagtggg ccgtgaaatt
    30421 ctggtgatta tcgacgaagt ggacgaagaa ggtgcgattg gtcgcagcat ggcagatgca
    30481 ccggaaatcg acggcgcggt ttatctcaat ggtgaaacca acgttaagcc gggtgatatc
    30541 ctgcgtgtga aagtcgagca cgccgatgag tacgatttgt ggggtagccg ggtttaatta
    30601 atcttaaggc agatctaaca aggatattgt tcttcgatct gcctctgcta cccttcccga
    30661 tgtcctaccc ttacgcacaa aatcccctga cgcataaatc gagaaaaatg aatcgtactc
    30721 gaggcaactc tcactttttc acaccaaatt aaagttttga tcgcaattac ttgaaataag
    30781 cgtaatggtt actatagtaa acattaccac aaggatggat gtatgggtgt gtataaggca
    30841 agacgattca gccaaagcac gaaaaaactt ggtattcacg ataaagttct catggctgcc
    30901 gctgaagaag tgattcaggg catatgggag gctgatttag gtagcggtgt catcaaaaaa
    30961 cgtttacccc tccaacaagg aaagagtggt ggagccagaa caattatctt ttttaaatca
    31021 gcaaatcatg tgttttttta tgatggttgg tccaagagtg gtctaagcag taaaggaacc
    31081 aaagaaatcg aagatgatga actggcggcc tacaaaaaaa tggcaaatgc atttctggcc
    31141 tttagcaata agcaaataga ggatttgatc gaaactggct ttttgattga ggtgaaaaat
    31201 gagcgataaa ttacaaatga tgctggaaga tgcaaaggaa ctgaatgcac tgggcctgat
    31261 gtctgatagt gaagttgatg ccattgccgt catggtcaaa gctcgtgaag tcagcgcccg
    31321 tatagccaaa gtcaaatcaa tgacgggtga agagatcaaa gccatccgca cccgatacgg
    31381 catgtcacaa tccgtgctgg cacatacaat gggaatgtca aaagaaagtg tctcgaaatg
    31441 ggaacgtaat gaaataaaac cgagcggccc ggcattacgt attttgaata cattggcaat
    31501 aaaagggccg gaagtttttg caaagtaaaa tgtttgcaaa tgatttacca cattcattag
    31561 taccccttga ttaaggggta ctaacactat tctaattcta ctgccagcta atctgcgccc
    31621 tgccgttttt cttactctta tacagtcgct catccgccag cattttcaag cgttcgacgg
    31681 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
    31741 ggaaatgaac aatttctttt agaatcgata gcaactcttc cagcgttcca cccgtataaa
    31801 tcaccgcaaa ttcgtcaccg ccgaagcggt agactttacc tttatctacg gggagctgtg
    31861 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
    31921 cgttaatgtt tttgaattta tcgacgtcaa acaggcacaa ataaccgcct ttttccggca
    31981 gcgcttttaa atcttcgtca aaggattttc tgcccatcgc tttggtcaga tcgtccagga
    32041 atgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
    32101 catccagtac gcgttcaaaa tggttattaa taatggcttc tttctccaca ataccacgat
    32161 taatcactct gcgtaccaga tagaggcaac tggcacccga tatcactgcc accaatgcgg
    32221 caaagaaaat aatcaatgta catacctgaa aataaacgtc ttttttataa gcaaatacgt
    32281 acaacccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacagtg aagatggcgt
    32341 ttttagcgtt atattcatca ccggtcaccg atttaccgtt cttgaacagg gagaacatca
    32401 ggttctcgct atccatcgcc agcgattttg acagtaaaga aaaacgttcc agaccaagca
    32461 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccat
    32521 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
    32581 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
    32641 gaccgacatc gtacaaataa gagggtgaaa ccgagtcggc gtaagctgtg cgtaagccaa
    32701 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
    32761 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
    32821 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
    32881 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
    32941 tttactaatt ttccactttt atcccaggcg gagaatggca tgggtttctg gtacagatac
    33001 ccctgagcct gaacacatcc taatttgatt aattcgcgcg caacgtcttc gctctctacg
    33061 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
    33121 tttcccgcag taccaaaaat atcgccggta aactgcgagt ccacctttat gctgtctgga
    33181 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
    33241 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctca
    33301 atcacggttt cagtgatctc aacacaaagg ctgttcgcct tcaggccatt ttctttcatg
    33361 atggtagtaa aacgctcatg aaagtgtggt tcgtttaact gccagggcga aacattaata
    33421 tgcagtaaga aatcatcctt cagcgacgca cgctgactaa aatgtctgaa ttcacccatc
    33481 gcggttttaa taatttgata gcccagctca ttgataaacc cgatatcttc agcaatggta
    33541 atgaacttca gcggtgaaat gatcccccgc tccgcagaca cccaacggca caacgcctcc
    33601 gcgcccagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
    33661 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
    33721 agtttcgcat catcacagaa aataaactct gagaacatat ggttgcggat actggaaatc
    33781 gccagtttca gcttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaata
    33841 gcagacttat ggatgcgcag attgcggtag ccaaaactgc tgtcaaaatc gtttaccgtc
    33901 gctatcttac gggtaaacgt ctgtacattt tcccggcaca ggagtaaata gagatcggca
    33961 ttaaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
    34021 ctgacgattt tattgcgttc atcaacacca aacaccgctt ccacagcttc cagaccacac
    34081 agttagatga tccccacagc cagatactga ttattgttgg attcaataat cgccatattt
    34141 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
    34201 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
    34261 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
    34321 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt cattaaataa
    34381 accaccaaca atacgataat aatgaacgca aacggcagat aaaaaagttt atctgatgcg
    34441 ttcaataaat taccaccagg ataataaaaa tagatttcga ataatgcgtt gtttggcacc
    34501 gctgatgttt tttcaatata ttccgtatcg acagaatcaa taatattata attaccttcg
    34561 ctattataaa taatcaattt ttcacgcgcg agttgattct tggcaatgat ctcatgacct
    34621 tgctttaaaa atagaacttc atcattagcg ctgatgtgat caagatactc gttcagatat
    34681 cgccagttta aatcgacatg aattttatcc accactaaca aatcgtgatc tttgccgttg
    34741 atgcgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
    34801 ttccagctgt tgttagagtc atctgaagcc atcgaccacg gtcgtttcga tgggttaaaa
    34861 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
    34921 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
    34981 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaattac gg
//
LOCUS       DAZKGF010000027        34118 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZKGF010000027 DAZKGF010000000
VERSION     DAZKGF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.027"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1557)
                     /gene="ycjM"
                     /locus_tag="ACHOQV_001339"
     CDS             complement(<1..1557)
                     /gene="ycjM"
                     /locus_tag="ACHOQV_001339"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HFP9629736.1"
                     /translation="MEQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSTFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYIRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     IDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELNNKATLRYLIYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLTGLFN"
     gene            complement(1771..2085)
                     /gene="pspE"
                     /locus_tag="ACHOQV_001340"
     CDS             complement(1771..2085)
                     /gene="pspE"
                     /locus_tag="ACHOQV_001340"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFP9629737.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(2160..2381)
                     /gene="pspD"
                     /locus_tag="ACHOQV_001341"
     CDS             complement(2160..2381)
                     /gene="pspD"
                     /locus_tag="ACHOQV_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFP9629738.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(2390..2749)
                     /gene="pspC"
                     /locus_tag="ACHOQV_001342"
     CDS             complement(2390..2749)
                     /gene="pspC"
                     /locus_tag="ACHOQV_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFP9629739.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2749..2973)
                     /gene="pspB"
                     /locus_tag="ACHOQV_001343"
     CDS             complement(2749..2973)
                     /gene="pspB"
                     /locus_tag="ACHOQV_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFP9629740.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(3027..3695)
                     /gene="pspA"
                     /locus_tag="ACHOQV_001344"
     CDS             complement(3027..3695)
                     /gene="pspA"
                     /locus_tag="ACHOQV_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFP9629741.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3862..4839
                     /gene="pspF"
                     /locus_tag="ACHOQV_001345"
     CDS             3862..4839
                     /gene="pspF"
                     /locus_tag="ACHOQV_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFP9629742.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4959..6224)
                     /gene="puuE"
                     /locus_tag="ACHOQV_001346"
     CDS             complement(4959..6224)
                     /gene="puuE"
                     /locus_tag="ACHOQV_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFP9629743.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKMFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDEAMKILQDALSD"
     gene            complement(6262..7542)
                     /gene="puuB"
                     /locus_tag="ACHOQV_001347"
     CDS             complement(6262..7542)
                     /gene="puuB"
                     /locus_tag="ACHOQV_001347"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFP9629744.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVHELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7544..9031)
                     /gene="puuC"
                     /locus_tag="ACHOQV_001348"
     CDS             complement(7544..9031)
                     /gene="puuC"
                     /locus_tag="ACHOQV_001348"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFP9629745.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVEVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(9181..9738)
                     /gene="puuR"
                     /locus_tag="ACHOQV_001349"
     CDS             complement(9181..9738)
                     /gene="puuR"
                     /locus_tag="ACHOQV_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFP9629746.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9765..10529)
                     /gene="puuD"
                     /locus_tag="ACHOQV_001350"
     CDS             complement(9765..10529)
                     /gene="puuD"
                     /locus_tag="ACHOQV_001350"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFP9629747.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            10741..12159
                     /gene="puuA"
                     /locus_tag="ACHOQV_001351"
     CDS             10741..12159
                     /gene="puuA"
                     /locus_tag="ACHOQV_001351"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFP9629748.1"
                     /translation="METNIVEVENFVQQSEERRGSSFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            12308..12472
                     /locus_tag="ACHOQV_001352"
     CDS             12308..12472
                     /locus_tag="ACHOQV_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629749.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12462..13847
                     /gene="puuP"
                     /locus_tag="ACHOQV_001353"
     CDS             12462..13847
                     /gene="puuP"
                     /locus_tag="ACHOQV_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFP9629750.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            13981..14226
                     /gene="ymjA"
                     /locus_tag="ACHOQV_001354"
     CDS             13981..14226
                     /gene="ymjA"
                     /locus_tag="ACHOQV_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFP9629751.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLALYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14539..16182
                     /gene="sapA"
                     /locus_tag="ACHOQV_001355"
     CDS             14539..16182
                     /gene="sapA"
                     /locus_tag="ACHOQV_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFP9629752.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            16179..17144
                     /gene="sapB"
                     /locus_tag="ACHOQV_001356"
     CDS             16179..17144
                     /gene="sapB"
                     /locus_tag="ACHOQV_001356"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFP9629753.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            17131..18021
                     /gene="sapC"
                     /locus_tag="ACHOQV_001357"
     CDS             17131..18021
                     /gene="sapC"
                     /locus_tag="ACHOQV_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFP9629754.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            18021..19013
                     /gene="sapD"
                     /locus_tag="ACHOQV_001358"
     CDS             18021..19013
                     /gene="sapD"
                     /locus_tag="ACHOQV_001358"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFP9629755.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            19015..19821
                     /gene="sapF"
                     /locus_tag="ACHOQV_001359"
     CDS             19015..19821
                     /gene="sapF"
                     /locus_tag="ACHOQV_001359"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFP9629756.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(19870..21033)
                     /locus_tag="ACHOQV_001360"
     CDS             complement(19870..21033)
                     /locus_tag="ACHOQV_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFP9629757.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFIRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(21042..22415)
                     /locus_tag="ACHOQV_001361"
     CDS             complement(21042..22415)
                     /locus_tag="ACHOQV_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFP9629758.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAAQETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQIGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTDGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(22419..25526)
                     /locus_tag="ACHOQV_001362"
     CDS             complement(22419..25526)
                     /locus_tag="ACHOQV_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFP9629759.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(25526..26647)
                     /locus_tag="ACHOQV_001363"
     CDS             complement(25526..26647)
                     /locus_tag="ACHOQV_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP9629760.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(26796..27362)
                     /locus_tag="ACHOQV_001364"
     CDS             complement(26796..27362)
                     /locus_tag="ACHOQV_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9629761.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            27840..28628
                     /gene="fabI"
                     /locus_tag="ACHOQV_001365"
     CDS             27840..28628
                     /gene="fabI"
                     /locus_tag="ACHOQV_001365"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP9629762.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            28772..29899
                     /gene="yciW"
                     /locus_tag="ACHOQV_001366"
     CDS             28772..29899
                     /gene="yciW"
                     /locus_tag="ACHOQV_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFP9629763.1"
                     /translation="MEKRHITGKSYWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARSIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFATCMNSSNEYKKISALLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            29967..31901
                     /gene="rnb"
                     /locus_tag="ACHOQV_001367"
     CDS             29967..31901
                     /gene="rnb"
                     /locus_tag="ACHOQV_001367"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFP9629764.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDLVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(31978..32082)
                     /locus_tag="ACHOQV_001368"
     CDS             complement(31978..32082)
                     /locus_tag="ACHOQV_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9629765.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            32136..>34118
                     /gene="pdeR"
                     /locus_tag="ACHOQV_001369"
     CDS             32136..>34118
                     /gene="pdeR"
                     /locus_tag="ACHOQV_001369"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP9629766.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         8042 a         9010 c         8597 g         8469 t
ORIGIN      
        1 attaaacaat cccgtcaggc aatcgccatc cgcattgctt ctttgaattc gcattacaaa
       61 tgaattaatc gcatcaatgg tgaaattatt atccggatga aactctttat gactgcgacg
      121 aagtgtaatt aaatgcgaca acttataata tatcaaatac cttaatgtcg ctttattgtt
      181 cagttctgtc gttatctctt tactagagta ttttttacgg ttaatcgcac gattatatcc
      241 aagtttttcg acacctgcgt aatcattacg cgaccccaga atgctttgaa tatatatcgc
      301 tggcacaccg gggaaactta acaaaatcgc atgggcgaga ataaacctgg cacaccgttc
      361 ttcgtcgcta cactcacggc ggctcaacgc atccatatag gtgacattca tttcataggg
      421 gctacgcgta ccgtcgggat tatttttcca gtttactaat gccccttcct gctgtaacac
      481 ctcgaccagc gctaatattt cgttttcggg caacaacccc cgcagtgggt taagcccgat
      541 gccatcgtga gaggcaagga agttaaacca ggtggtgttg ctggaaggta gtgtcaggtt
      601 ttgcgcccac gcacaaagtg cctcaacgtt ctgtttttgc accgcatgca gcaccagcgg
      661 cggcagcgag aactggtaca ccatatgcgc ttcgtcatca ccgttgccaa agtaagcaat
      721 gttgtctata tgcggaacat tggtctcggt aatgatcact gtgcctggcg caacgttatc
      781 aataatcgac cgtaacagtt tgataatcag atgtgttttt tccagatgga tgcagcttgt
      841 tcccggctct ttccacataa agccaacggc atccagacgg atatactcag cacctttctc
      901 aaggtaacac agtaaaacat ccaccatcgc cagtaacact tcagggctac ggtaattgag
      961 gtcaatttga tcgtcgctaa aggtggtcca taaatgacgc gttgaattat cgcgcatctg
     1021 gaatggcgtt aataacggta acgcacgcgg acgggtgacg gcgctgagat cggtttgcgg
     1081 atcaaccgca ataaaaaaat cttcaaaacc tggctgctgt tgtaaatagt ttttaaacca
     1141 ttcacttttt gccgacatat ggttgcagac aaaatcaaac attaaatgac tgcattcacc
     1201 gagttgctga atatcctgcc actcccccgc ttcactggcg acctgatgat aatcaattac
     1261 cgaaaagcca tcatcagatg accatggata aaacggcaac aaatgaacat gtgaaaaagt
     1321 gctttgcagc cattgatggt aaaactgatt aaatgtgggt agtggtttta aatcattact
     1381 gtgaaattga tcggcatagg taattaacac gacatcactt tcatcccagt gttttttacg
     1441 tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt acaagtttct gtaaatgagt
     1501 cgcggtaaat gttccaccgt agatttcgtc caggtaatcc gtaattttct gttccatgtt
     1561 gccttttcgt ggtagcggtc ccatgaaacg agagactact ccgcagaaaa taaactgtca
     1621 attgagcaca agggttttag tcacgcagga aatcattttt taccagggaa aaagcgtatt
     1681 tgtgagctga cgcaagtaat ctatggggat gtaaataagg tgtgacagaa aaagtaaacg
     1741 gcgcataagc gccgcttagg gtggattctt ttaacctttg accttcggca ttgcgatgtc
     1801 tttcaggccg ccggcgttct caacgtgggt atatcccatc tcgctaagga tctcttttgc
     1861 ctgccctgac tggcgtccgg cattgcaata cactttcacg gtgtcgtttt tatccggaac
     1921 ggcggtggca atgcgctctt tcacttcctt caggggaata ttgatggctc cctgaacgtg
     1981 ctcttgctga tactgctctg gaacacgaac atcgatccag tgttcagcgg cgaaaacggg
     2041 cagtgaaaac accagtgtca gagcaagtaa gcctttttta aacatgtaag cgtccttcta
     2101 tggatgaaaa ttgccatcca taatacgcct tagctttaac aatttttgcg aaactcccct
     2161 caccttttat aacgctgcgc cagtttattg gcagcccgac ttaacagcgg ttccagcgcc
     2221 acagccagca acattttcag tggtcggcga gcaactgatt ttatcgccca gccagctaca
     2281 cccgccgggc catagcgcag tgcggtaaga agtaccagct tgcctgctaa tttgaaacca
     2341 ggcttcacct tttgcccggc ctgttgccag cgagtattca taactttcct cacagttgac
     2401 ggaaacggct acgtaacgtg aaagtatcgg aagtgacata acgttccatc tcgcgtaaac
     2461 gcgtttcact tgccgccagt tcacggtcga cttcatccag caattcactg ctggaaggta
     2521 gctgctcacc aaaggccatg ttgtccggca ttggatcaag cgcaaatgac aaaatgatgt
     2581 aagcaaccag ggtaaacagc gccagaccga agaaaatcga cagcaccacc aggatacgca
     2641 ccagttttac tggtacatca aaatagttgg caatcccggc gcagacgcca cggaccatgc
     2701 cctgctgtgg aatacgccat aattttttat tgagattaat gcccgccatt agcgatccct
     2761 ccagttcgga tgttctgcat caagaatgga ttccagcgcc tgaatacgtt cgcgcatccg
     2821 ttttgcttca tcagccagtt gcgctaatcg ctgctgctca ctttgcgaca attcactgcg
     2881 accagaacga ttgctgtaat gcagccataa ccagatcggt aaaacaaaca gcacaaaaat
     2941 ggttaacgga atagccagaa atagcgcgct cataaggact ccttagacga tgagcggcgg
     3001 cgcgttcgga cgccgcctgg atgttattat tgattgtctt gcttcatttt ggcttttaat
     3061 tgtgccagtt gctcgctgat tgcatcatcg gctttcagtt cggcaaactg atcgtccagc
     3121 gatttttgtt taccgaagct gtggctttct gcttccgctt ccatctggtc aattcgacgt
     3181 tcgaaagatt cgaaacgagc cattgcttca tccagtttgc cactgtccag ctgacgacgc
     3241 acatcgcgcg acgagtttgc cgcctgatgg cgtaacatca atgcctgctg gcgagcgcgt
     3301 gtttcgctca atttgttttc cagctcgccg atctctttct tcatgcgtgc cagcgtatcg
     3361 tccaccagcg tcacttcatg ttccaggctc ttaatcagat cggtcagttt ctgtttttca
     3421 attaacgccg cacgcgccag gtcttctctc tctttcagca gcgccagttc ggctttttcc
     3481 tgccattcaa cttcacgcgc cgacgcttgt tcaatacggc gagtcagctg tttcttttct
     3541 gccagcgcac gcgccgaagt agaacgtact tcaaccagtg tatcttccat ctcctggatc
     3601 atcagacgca ccagtttctg tggatcttcc gctttctcta acagagcgtt gatgttggca
     3661 ttcacgatgt cggcaaagcg agaaaaaata cccataatgt tgtcctcttg atttctgcgt
     3721 tcaggattgt cctgctgaac tattaataca atttgcgtgc caatttttta tctttttgat
     3781 ttataaagat ctgattgaag aatcaacagc aacatgccag gatgagttag cgaattacac
     3841 taacaagtgg cgaatttcat catggcagaa tacaaagata atttactcgg tgaggcgaac
     3901 agctttctcg aagtgctgga acaggtttcg catctcgcac cgctggataa accggtgctc
     3961 atcatcggcg agcgcggcac aggtaaagag ctgattgcca gccgtctgca ttatctctcc
     4021 tcccgttggc aggggccgtt tatttccctt aactgcgcgg cgttaaatga aaatctgctg
     4081 gattccgaac tgtttggtca cgaagcgggg gcgtttaccg gtgcgcaaaa acgtcatcca
     4141 gggagatttg aacgtgccga cggcggtacg ctatttcttg atgaactcgc tacggcaccg
     4201 atgatggtgc aggagaaatt attgcgcgtg attgagtacg gtgaactgga gcgcgttggc
     4261 ggcagccagc cattgcaggt gaatgtacgg ttggtatgcg cgacgaatgc cgatctcccg
     4321 gcgatggtca atgaaggtac ttttcgcgct gacctgctcg accggctggc tttcgatgta
     4381 gtgcaactgc cgcccctgcg cgagcgcgaa agcgacatta tgctgatggc agaacacttt
     4441 gccattcaga tgtgtcggga aatcaagctg cctctgttcc cgggttttac ggagcgcgcc
     4501 agagaaacat tgctgaatta tcgctggccg ggaaatattc gtgaattgaa aaacgtggtg
     4561 gaacgttcgg tgtatcgcca cggcaccagc gattatccgc ttgatgacat cattattgat
     4621 ccctttaaac ggcgtccgcc tgaagacgct atcgccgttt cagaaaccac ctcgcttcca
     4681 acgctgccgc tggatttacg tgagtttcag atgcagcagg aaaaagagtt gctgcaactc
     4741 agtttgcaac aagggaaata taaccagaag cgcgcggctg aattactggg gttaacctat
     4801 catcagttcc gcgcgttgtt gaaaaagcac cagatttagc gaagttgggc aatacaactt
     4861 gccggatgcg gcgtgaacgc cttatccggc atacataatc aatacgttgt ttgcaggcct
     4921 gataagcgca gcgcatcaaa cattattgcg ttgggctatt aatcgctcag cgcatcctgc
     4981 aaaattttca ttgcctcatc gaattgcgca tccgggatgg tcagcggata caggaagcga
     5041 atcacgttgc cgtatgcgcc acaggtcagc aagagcagcc cctgcgccag cgcgcgttgc
     5101 tggattttct gtgcaatcgc cgctgacggc tcgcccgttt gcggatcgtt aaactctgcc
     5161 gcaatcatag accccagacc gcgtaccgcc gcgatggccg gaacgctttc tttggcatca
     5221 atcaacgtgt ttgtcaggcg ctggcccagt tgattcgcgc gttcacagag tgattctttg
     5281 tcgataatgt tgagcacagc gtgcgcggca gccaccgcca gcgggttccc ggcgtaggta
     5341 ccacccaacc cgcccggcgc gggcgcgtcc ataatattcg cgttaccgac cacgcccgaa
     5401 agcggcatcc cgcctgcgag gcttttcgcc atcgtcatta agtccggctt atcggcgtag
     5461 tgatccatgg caaacatttt accggtacgc gcaaagccgc tttgcacttc atcggcaatc
     5521 atcacaatgc cgtgctcgtc gcacaggcgg cgaatggcgg caaccagctc ttttggcgca
     5581 acgttgaaac cgccttcgcc ctgaaccggt tcgaaaataa tcgccgccac ctgcttcgct
     5641 tcaatgtccg atttaaacaa gcgttcgatg gcgtcgaggg agtcctgcgt tgaaatgccg
     5701 tgtaaatctg acggataagg tacgtgatat accgaaccgg ggaacgggcc gaagccgatt
     5761 ttgtacggcg cgacctttcc ggtcagcgcc atggtcatat atgtacgacc gtggaagccg
     5821 ccgctaaacg caatcacgcc agggcgtccg gtatgggcgc gggcgatttt caccgcgttc
     5881 tccaccgctt ccgcaccggt ggtgaagaac gcagttttag cctgcccgct caccggggca
     5941 agggcgttaa ttttctccgc cagcgtgacg tagctttcgt acggcacaat ctgatacgcc
     6001 gtgtgggtaa actgttgcaa ttgctgttcc accgccgcga ccagatcagg gtggcgatgc
     6061 ccggtattca gtaccgcaat gcctgcggcg aaatcgatat attcgttgcc ctctacatcc
     6121 ttcagcgtgg cgttttctgc cgactgggcg aagaagttac acatcacgcc aaccccgcgt
     6181 ggggtagcag aaagacgacg ctgatggaat tcattgttgc tcatgtttca caccttttga
     6241 ctcttcactg ttaatcggaa attaaacgcc cagacgatcg cgcaggctgt aataagccgc
     6301 gcccatcgcg gtaaacggaa cgcgcagcgt gcgcccgccg gggaacgggt aatgcggcag
     6361 attggcgaag gcgtcgaaac gctcggcgtc accgcgcagc agttcagcaa tcaaacgccc
     6421 ggcgaggtgg gtacaggtga cgccgtggcc gctgtagccc tgcatgtagt agatgttggt
     6481 atcgaggcga ccaaactgcg gcatgcgtga cagggtcagc aggaagttgc ccgtccagcg
     6541 gtaatcaatt ttcacgccct tcagttgcgg gaaggttttc agcagtttcg gcaccacaag
     6601 gcgctcaaca tcgtccgggt cgcgtgcgcc gtagaccacg ccgccgccgt acagcaggcg
     6661 gttatcggcg gtaagacggt agtaatcaag cagatagtta cagtcttcca cacagtagtt
     6721 tttcgggatc agcgaacggg ctaaatcttc cgacagccgt tcggtggtga tcacctgcgt
     6781 gccgcacggc atgctgcgtt tcgctagttc cggctctact ttatcgccca ggtaggcatt
     6841 cccggcgaca atcacatact tcgccgtcac ctgaccattg gcagtccgca ccacggctgg
     6901 cgtggtgtgc tggatctgcg tcactgcgga aagctcatgc acgcgcccgc cgttcaggcg
     6961 gatggcatcg gcttcaccaa tggcgaggtt tagcgggtga atatgcccac cgctgtgatc
     7021 cagcagcgca ccagtgtagc gatcgctggc gacctcgcgg cgaatagcgt tggcgtccag
     7081 cagctccagc tgtttattgc cgtagcgttc ccagttctct ttctgctctt cgagtgtggc
     7141 gagctgttta tcattcatcg ccacaaacag accgccgggg cgatagtcgc agtcaatctg
     7201 ataacgtttg atacgttcgc ggatgatctc gccaccctcg aacatcatgc tgccgagcat
     7261 acgggcggta tccatgccgt agcttttttc gatcacgtcg atgtcgcggc tgtaggagtt
     7321 cacaagctgc ccgccgttgc gtccgctcgc gccaaagccg atgcgtgagg cttcgagaac
     7381 caccacgtca aagcccgctt ccgccagatg cagcgcggag gaaagcccgg tatagccgcc
     7441 gccgaccacg caaacgtcgc aggaaatcga ctcattcagc gtgtcgaatg gcgcgtattt
     7501 attcgcactg gcggcgtagt aactgctggt gtgttcggtc atttcaggcc tccaggctta
     7561 tccagatggt tttcagttcg gtgaattttt caagggcatg cagggatttg tcgcgcccgt
     7621 tgccgctctg cttatagccg ccaaacggca cggtcatatc gccgtcgttg tagttattga
     7681 cgaagacgga gccggctttc aggcggcgac tcatgcggtg cgcgcgggag aggtcgcgcg
     7741 tccataccgc cgcgccaagt ccgtactggc tgtcgttggc aagctgtagc gcctgttctt
     7801 ctgatgtgaa acgcgtgacc accagcaccg gaccgaaaat ctcttcgcgg cttaaggacg
     7861 cattcgggtc tacctccaca aagatggtcg ggccgatggc ggcagccagc tcggcgttac
     7921 ggccatccag cagcagttgc cctttgcttt cgccttcccg aataaagcta tggaccgagt
     7981 cggcgtgggc gcagtcgatt aaggtgccca tggtggttgc gggatcaagc ggatggcccg
     8041 gctgccagtt ttgcgcttgc tgttttaaca gggctaagaa ttcatcggcg atgctctctt
     8101 ccagcaacag gcgcgttccg gcgatacaca cctgcccctg gttgtagaaa atgcccgcag
     8161 cggtcgcgct cgccgctttt tgcaaatccg gacagtcggc gaaaacgatg ttggcgcttt
     8221 tgccgcccgc ttccagccag acgcgtttca tgttgctgtc gcccgcgtcc ttcagcagct
     8281 gtttcccggt gcgggttgag ccagtaaagg caatggcatc gatatcgtta tggcgcgaaa
     8341 gcgcctgccc ggcttcatga ccaaaacccg tcaccacgtt caacacacca tccggcagcc
     8401 ccgcttcttt cgccagcccc gcgagacgaa tcgcactgag cggtgatttt tcagacggtt
     8461 ttagaatcac gctgtttccc gccgccagcg ccgggccgag tttccagcaa gtcagcagca
     8521 gcgggaagtt ccacggcacg atggcggcaa tcacgccgac cggttcacgc acgatcatcg
     8581 ccagctcatg gctactggtg gtcgccactt cgccatacac tttgtcgatc gcttcggcgt
     8641 accagcgaat ggcgcgcgcc gcgccgggaa tatcatcacg caggctgtga cgaatcggtt
     8701 taccggtgtc gagcgtttcc agcagcgcca gttcttcagc gttggcttcc attaaatcgg
     8761 cgagtttatt cagcaccgct ttacgcttag ccggagaaga gagtgaccag tcgccacgtt
     8821 caaacacgcc gcgtgctgcg ctcaccgcac ggtcaatatc gacgctcttg ccgcgggcaa
     8881 tattcgccag cggtgcctgg gtgaccggat caacggtttc aaaagtttca ttttccgccg
     8941 cagcagtata ttcaccgtta ataaataagc gggtttcaat ggcgagactt aacgctttat
     9001 cctgccagta agccagatga tgaaaattca ttatgactcc tgtttcacgt ctatcagata
     9061 tatgcagttt gtgaacgtca gggcgcagag atccccgggc cgtatgcccg tttatttctg
     9121 gcgacattct ttatagattg ccgggcgcat tgcgcttacc cggcttacag aacaaaaaga
     9181 ttaaaacgtg gtgggcgtat gggcgctgat aattcggcaa atacctgccg acgtattact
     9241 gaaactgtgc gggatgccgg tattaatggc ataactttgc cccgcgacga gatgataatc
     9301 ctgaccatta atcgtcagaa caatttcacc ttccagtaca gtgcctattt cctcaccctg
     9361 atgcttaatt ctttccccag tggttgtgcc cggctggtaa gtttcaaaga tcatcgccag
     9421 cgtgcgattc gggttaccgt tatgaaccag cttcattgac acaccctgac tgcccatctc
     9481 aattaagtcg tcctgattaa tgacgacctg cggctcatca ggtttttccg gctcggaaaa
     9541 gaattccgag agtgacagac catacacctt cagcagcttt tgcagcgtac tgatggcagg
     9601 gctgacttta tcttgttcta tcgtactgat agcactgtga gtcagcccgg agagttcggc
     9661 ggcacgacgt tgtgaaagcc cctgctgctg gcggatttcc gacaagcgtt ttcctggcgc
     9721 cagtccctca tcactcatat ttgcatttcc ttaaactgta gtggtcagag tcgctgtttt
     9781 tcagcgatat ggtgctgaca agcggtgata aagccctcga acaatatacg cgaaagcgcg
     9841 tactcgctac tgttccattc cgggtgccac tgtacgccca gcgcaaaagg atgattgatg
     9901 acgctaaccg cctcaactaa gccatccggc gagcgagctt caacacgcaa ccgtgggcta
     9961 acgaccttcg ccccttgtcc atgcagagag tttacccaaa agttgctaca ttcaggtaac
    10021 aacgcagaca gtaatccccc ctcttcaacc tgaacttcat gcgacggcgc atattgctgc
    10081 tccaccggca gttcgggatc ttcccgatgt tccagcaatt caggctgttc gcacagcttg
    10141 cgatgcaacg aaccaccggt tgccaccacc agttcctgta aaccccggca gatggcgaaa
    10201 atggggatgc gcctttcgag tgcggcatta attaacgcca tgctcagaag atcacgccca
    10261 ggatcggcgt caggctcatc gccgttttca ccatataggt gcggctgcac attgctggga
    10321 ctaccaggaa gataaatgcc atcgagtttc ggcaaaagtt gttcaagtaa tgacggttcc
    10381 gccagcgcat gtggcagcgc aattggcaag ccgcctgcgt ggatgatggc attcaggtac
    10441 ttttcttgca gagtctgggt cgcatgaccc ttaagcctgt tcctgcacat tacgacaccg
    10501 ataaccggat tgttcattat attttccatg ctcaatctca caaagtggac taaattatcg
    10561 ccattactgc gctttaaatg acctttatgt tcaatatttt ttcaatctag cagtgggttt
    10621 tcatttttgc aaactcaatt taacatttga aaaacattta gtttgcatac agattcgaat
    10681 agtggtcatt atattttacg ctttgataac gagcggaaaa caaaccaaag gcgaagaatc
    10741 atggaaacca atatcgttga agtagagaac tttgttcagc agtcagaaga gaggcggggt
    10801 agctccttta cgcaggaagt gaaacgctac ctggagcgct acccgaatac gcaatatgtt
    10861 gatgttttac ttaccgattt gaatggttgc ttccgtggaa aacgtattcc tgtttcaagc
    10921 ctgaagaagc tcgagaaagg gtgttatttc ccggcctcgg tgtttgcaat ggatattctg
    10981 ggcaacgtag tagaagaagc gggcctgggt caggaaatgg gcgagccgga tcgtacctgt
    11041 gttcctgttc tcggttcctt aaccccttct gccgccgatc cagagtttat cggtcagatg
    11101 ctcctgacca tggtcgatga agatggcgct ccctttgacg ttgagccgcg gaacgttctt
    11161 aaccgcctgt ggcagcagct gcgccagcgc ggattgttcc cggtcgtagc ggtagagctg
    11221 gagttctatt tactggatcg ccagcgcgac gctgaaggat atctgcaacc gccctgcgcg
    11281 cccggcaccg atgaccgcaa tacacaaagc caggtttact ccgttgataa cctcaaccac
    11341 ttcgccgacg tgctcaatga tattgatgag ctggcgcagt tacagctgat tccggcagat
    11401 ggcgcggtcg ccgaggcctc gccaggtcag tttgaaatca atctttacca taccgataac
    11461 gtgctggaag cttgcgatga tgcgctggca ctaaaacgcc tggtgcgtct gatggcagaa
    11521 aagcataaga tgcacgccac ttttatggcg aagccgtatg aagagcacgc gggcagcggg
    11581 atgcatatcc atatcagtat gcaaaataat cgtggcgaga acgtgctttc tgacgcggaa
    11641 ggcgaagatt cgccgctgct gaaaaagatg ctcgccggga tgattgacct gatgccgtca
    11701 tcaatggcgt tgctggcacc aaacgtgaac tcgtatcgcc gcttccagcc ggggatgtat
    11761 gtaccgacgc aggcgtcgtg gggccataac aaccgcaccg tcgccctgcg tattccctgc
    11821 ggcgatcgcc ataatcaccg cgtggaatat cgcgtggcgg gtgccgatgc caacccgtat
    11881 ctggtgatgg cggcgatttt tgccggtatt ttgcatggcc ttgataacga actgccatta
    11941 caggaagaag tcgaaggtaa cgggctggaa caggaaggct tacccttccc gattcgccag
    12001 agcgatgccc tgggtgagtt tatcgagaat gatcacctgc gccgctattt aggcgaacgc
    12061 ttctgccatg tctatcacgc ctgtaaaaat gatgagctgt tgcagtttga gcgcctcatc
    12121 acagaaacag aaattgagtg gatgttgaaa aacgcgtaat accccttgcg gggttgtttc
    12181 atcggcctga gcggagccag gcctgacatt tgctggtaat ccagacattt cccggatgtc
    12241 gcgttatgga gcgcaggcgg caacgggctt ccgttaacga ttaagactgt gacgaggaaa
    12301 tgagatgatg ccgatgatta aatccccgca tggcgaggga ggctgcgtat gcgcccctcc
    12361 tgcgactgac tggactccgc cccctctgct tcccttgcta aacaggtttg atttcaggtc
    12421 gacacgaccg caaaccttat tacgcagggg aggcagcaat tatggctatt aattcaccac
    12481 tgaatattgc tgcgcaacct ggcaaaaccc gtctgcgaaa atcactgaaa ttgtggcagg
    12541 tggtgatgat gggtctggcc tatctcacgc cgatgaccgt atttgatact tttggtattg
    12601 tctccggcat tagcgacggt cacgttccgg cgtcctattt gctggcgctg gcgggcgtgc
    12661 tgtttaccgc tatcagctac ggcaaactgg ttcgccagtt tccggaggcc ggttcggcct
    12721 atacctacgc gcaaaagtcg attaacccgc acgtcggatt tatggtcggc tggtcatcgc
    12781 tgctggatta tctctttttg ccgatgatca acgttttgtt ggcgaaaatc tatctctccg
    12841 ccctcttccc ggaagtgccg ccgtgggtgt gggtggtaac cttcgttgcc attttaaccg
    12901 ccgcgaatct gaagagcgtc aacctggtcg ctaacttcaa taccttgttt gtactggtgc
    12961 aaatctccat catggtggtg tttatcttcc tggttgttca gggactgcat aaaggagaag
    13021 gcgttggcac cgtctggtca cttcagccgt ttatcagcga gaacgcgcac ctgatcccaa
    13081 ttattaccgg ggcgacgatt gtctgtttct ccttcctcgg tttcgatgcg gtgaccacgc
    13141 tttcggaaga gacgccggac gccgcacgcg tgatcccgaa agccatcttc ctgacggcgg
    13201 tatacggcgg cgttatcttt atcgctgcgt cgttctttat gcagctgttc tttcccgata
    13261 tcagccgctt taaagacccg gacgccgcac tgcctgaaat tgcgctatac gtcggcggca
    13321 agctcttcca gtcgattttc ctctgtacca cgtttgtgaa cacgttagcg tctggcctgg
    13381 cgtcgcacgc cagcgtgtcg cgtctgctgt atgtgatggg gcgtgacaat gtgtttccgg
    13441 agcgcgtgtt tggctatgtg cacccgaaat ggcggactcc ggcactgaac gtcattatgg
    13501 tcgggattgt cgcgttgtca gcgctgttct ttgatttagt caccgcgaca gcattgatta
    13561 acttcggtgc tctggtggca tttaccttcg taaatctgtc ggtgtttaat catttctggc
    13621 ggcgtaaagg aatgaataaa agctggaagg atcacttcca ctatttgctg atgccgctgg
    13681 ttggcgcgct gacggtgggt gtgttgtggg ttaacctcga gtcaacgtca ctgacactcg
    13741 gtctggtatg ggcttcgtta ggcggcgcat atttgtggta tctgatccgc cgctatcgca
    13801 aagtgccgtt gtatgatggt gacagaacgc cggtgagcga aacgtaatag tcggatgcgc
    13861 ggcgcattat cctgcctgag ggtaatgcac cgcgccatta actataaaaa gatgtatgac
    13921 ccggatcacg agtgctatca tccgccctta tgcttagcgc attaacccca ggggcaaata
    13981 atgaaccacg atatcccgct aaaatatttt gatattgccg atgagtacgc gaccgaatgc
    14041 gcagagcctg tcgcagacgc tgaacgcaca ccgctggccc tctacttcca gttactgctc
    14101 acccgtttga tgaacaatga agagatcagc gaagaagccc aacatgaaat ggctgctgaa
    14161 gcggggatta atcccgtacg cattgatgag atcgcagagt ttttgaatca atggggtaat
    14221 gagtaattat ttcattgcag actctcggtc agtatcgttg tccggataaa gcgttcacgc
    14281 cgcatccggc taacaatgac acgcactgca gcgtgcctca atgggtttct cacgatcgta
    14341 ccgctctgct cctcgctaag ttgttctgtg agttatagtt atagagaaat cacgggtacg
    14401 aatgttgaac agatggcgac gtcatctgtt caacgaccaa ccacacggcc ctcattacga
    14461 cccctgaaca caaacggtaa tggcaaatgt cattaaatat tagtaagata gcctctttac
    14521 ataaaatcca ttaatattat gcgccaggta ttatcgtctc ttttggtgat tgctggactt
    14581 gtgagtggtc aggcaatcgc cgcgcctgaa tcccccccgc atgctgatat ccgcgacagc
    14641 ggttttgtct attgcgtcag cgggcaagtc aacaccttta acccatccaa agcgagcagt
    14701 gggttaattg tcgataccct tgccgcccag ttttatgatc gactgctgga tgtcgatccc
    14761 tatacctatc gcctgatgcc ggaacttgcc gaaagctggg aagtactcga caacggcgcg
    14821 acctatcgct tccacctgcg tcgcgatgtt ccgtttcaaa aaaccgactg gtttactccc
    14881 actcgtaaaa tgaatgccga cgatgtggtg tttaccttcc agcgaatttt tgaccgcaac
    14941 aacccgtggc ataacgtcaa cggcagcaac ttcccctact tcgacagcct gcaatttgcc
    15001 gataacgtga aaagcgtccg taaactggat aatcataccg ttgagttccg tctggctcag
    15061 ccggatgctt cttttttgtg gcacctcgca acccattatg cttcggtcat gtcggcagaa
    15121 tatgcccgga agttagagaa agaagatcgc caggagcaac tcgaccgtca gccggtcggc
    15181 actggaccgt atcagttgtc ggaataccgc gccgggcaat atattcgctt acaacgtcat
    15241 gatgatttct ggcgtggtaa gccgttgatg ccacaagtgg tggtggattt aggctctggc
    15301 ggtacaggac gattgtcgaa actcctgacc ggggagtgcg acgttctggc ctggcctgct
    15361 gccagtcagc tatccatttt gcgtgacgac ccacgcttgc gtttaacgct gcgcccgggg
    15421 atgaacgtcg cctatctggc gtttaacacc gctaaaccgc cgctaaataa ccccgctgtc
    15481 cgccatgcgc tggcactggc gattaataac cagcgcctga tgcaatccat ctattatggt
    15541 acggctgaaa cggcggcctc tattttaccg cgcgcctcgt gggcctatga caacgaggct
    15601 aaaattactg aatacaatcc ggcaaaatcg cgcgaacagt tgaaggcgtt ggggctggaa
    15661 aacttaacgc tgaaactgtg ggtgccaaca cgttcgcagg cgtggaaccc cagtccgctg
    15721 aaaaccgccg aactgattca ggcggatatg gcacaggttg gcgtaaaagt ggtgattgtg
    15781 ccggtagaag gtcgttttca ggaggcgcgg ttgatggata tgagccatga tctgacgtta
    15841 tccggttggg cgacggacag taacgacccg gacagtttct tccgtccgtt actgagctgc
    15901 gcggcaattc attcacagac caacctcgcc cactggtgcg atccgaaatt cgacagcgtg
    15961 ttgcgtaagg cgctctcctc gcagcagctg gcggcgcgta ttgaagccta tgacgaagcg
    16021 cagagtattc tggcgcagga attacccatt ctgccgctgg cgtcgtcatt gcgcttgcag
    16081 gcctatcggt acgatatcaa aggtctggta cttagcccgt ttggtaacgc ctcctttgct
    16141 ggggtgtatc gcgagaaaca ggatgaggtg aaaaagccat gattatcttc accttgcgcc
    16201 gtattttgtt attgattgtc accttgttcc tgctgacctt cgttggcttc agcttgagct
    16261 atttcacccc acatgcgccg ttacaaggcg cgtcactgtg gaatgcctgg gtgttctggt
    16321 ttaacggcct gatccactgg gattttggtg tttccagcat taacggtcaa ccgattgccg
    16381 aacagttaaa agaggtcttc ccggcgacga tggaactgtg catcctcgcc ttcggttttg
    16441 cgctgattgt tggcatcccc gtaggaatga ttgccgggat tacgcgccat aagtggcagg
    16501 ataacttgat caacgccatc gccttactgg gcttttcaat cccggtgttc tggctggcgc
    16561 ttctgctgac gctgttttgt tcactcacac taggttggtt gcccgtttca gggcgtttcg
    16621 atctgctcta cgaagtgaaa ccgattaccg gttttgcgtt gattgatgcc tggctttcgg
    16681 actcaccatg gcgggatgaa atggtcatga gcgcaatccg ccatatgata ttgcccgtga
    16741 tcaccctgtc ggttgcgcca acaactgaag tgatccgact gatgcgtatt agcactatcg
    16801 aagtgtatga ccagaactat gttaaagcag cggcgacccg cggtttgtcg cgctttacta
    16861 ttttgcgtcg ccacgttctg cataacgcgt tacctccggt tattcctcgc ctgggcttac
    16921 agttttctac catgctgacg ctagcgatga tcaccgaaat ggtttttagc tggccgggtt
    16981 tagggcgctg gttaattaac gccatccgcc agcaggatta cgcagccatt tccgccggag
    17041 tgatggtgtg tggctcactg gtcattattg ttaacgtgat ttctgatatt ttgggtgcca
    17101 tggctaaccc tctgaaacat aaggaatggt atgccttacg atagcgtata cagcgaaaag
    17161 cgcccgccgg gcacgctgcg taccgcctgg cgcaaatttt atagtgacgc ctctgcgatg
    17221 gtcggcctgt acggctgcgc ggggctggct gtactgtgta tttttggcgg ctggtttgcg
    17281 ccttacggca tcgaccagca atttctcggt taccaattat tgccgccgtc atggtcgcgc
    17341 tatggcgaag tttctttctt cctggggact gacgacctcg gacgcgatgt tttaagccgt
    17401 ttactgagcg gagctgcgcc aaccgtaggt ggcgcatttg tggtgacgct tgccgcgacg
    17461 atctgtggcc tggttctggg caccttcgcc ggggcgaccc acggcctacg ctcagcggtg
    17521 cttaaccata ttctggatac cttactggcg atcccttcgc tgctgctggc aattatcgtt
    17581 gtagcgtttg ccggaccgag tttgtctcac gccatgtttg ctgtctggct ggcattgctg
    17641 ccgcgtatgg tacgttcgat ttacagcatg gtgcatgacg aactggaaaa agagtacgtt
    17701 atcgccgccc gtctggatgg cgcatcaacg ctgaatattc tctggtttgc tgtgatgccc
    17761 aacatcaccg ccgggctggt caccgagatc acccgcgcac tgtcgatggc aattctcgat
    17821 atcgccgcgc tgggctttct cgatctcggc gcacaactcc cctcgcctga atggggagcg
    17881 atgctcggtg atgcgctgga actgatttat gtcgccccgt ggactgtcat gctgccaggt
    17941 gcggcaatta tgattagcgt gctgttggtt aacctccttg gcgacggtgt tcgtcgtgca
    18001 attattgcgg gggtggaata atgccattac tcgatattcg taacctgacc attgaattta
    18061 aaaccggtga tgagtgggtt aaagccgtcg accgcgtaag tatgacctta accgaaggtg
    18121 aaatccgcgg tcttgttggc gaatccggtt ccggcaaaag tttgattgcg aaagcgattt
    18181 gtggggtgaa taaagataac tggcgtgtta ctgctgaccg tatgcgtttt gatgatatcg
    18241 atttgctgcg tctctccgca cgcgaacggc gcaagctggt tggtcacaac gtgtcgatga
    18301 ttttccagga accgcagtct tgtcttgacc cttcagaacg tgtgggtcgc cagttgatgc
    18361 aaaacatccc agcctggacg tataaaggcc gttggtggca gcgttttggc tggcgcaaac
    18421 gccgtgccat tgaactactg caccgtgtgg ggattaaaga tcacaaagat gcgatgcgca
    18481 gttttcccta tgagttgacc gaaggtgaat gtcagaaagt gatgatagcc attgcactgg
    18541 cgaatcaacc gcgtctgctg attgctgacg aaccgacaaa ctcaatggag ccaacaaccc
    18601 aggcgcaaat ctttcgcctg ctgacgcgtc tcaaccagaa cagcaatacc actattttgc
    18661 ttatcagcca tgacttacaa atgcttagcc aatgggcgga taaaattaac gtgctttact
    18721 gcggtcaaac ggtggaaacc gcgccaagta aggagttggt gacaatgcca catcatcctt
    18781 atacccaggc gctgatccgc gcgataccag acttcggcag cgcgatgccg cataaaagtc
    18841 gcctcaatac gctgcccggc gctatcccac tgctggaaca gttaccgatt gggtgtcgtc
    18901 tggggccgcg ttgcccgtat gcacaacgag aatgcattgt gacgccacgt ttgacggggg
    18961 cgaaaaatca tctctatgcc tgtcacttcc cgctgaacat ggagaaagag tgagatgatc
    19021 gaaacgctgc ttgaagtgcg taatttaagt aaaacgttcc gttaccggac cggctggttt
    19081 cgtcgtcaga ccgtagaagc ggtaaaacct ttgagcttta cgctacgtga aggccagaca
    19141 ctggcgatta ttggtgagaa tgggtcgggt aaatccacac tggcaaaaat gctggcggga
    19201 atgatagaac cgaccagcgg cgagttattg attgacgatc atccgctgca ttttggcgat
    19261 tattccttcc gtagtcagcg tattcgcatg atttttcagg atccttcgac ctcgttgaat
    19321 ccccgtcaac gtatctcgca gattcttgat tttccactgc gcttgaacac cgatctggaa
    19381 cctgaacagc gtcgcaaaca gattattgaa acgatgcgga tggttgggct gctaccggat
    19441 cacgtcagtt actatccgca tatgttggcg cccggacaaa aacaacgtct gggtctggca
    19501 cgcgcgctga tattgcgtcc aaaagtgatt attgccgatg aagccctcgc ctcactggat
    19561 atgtcgatgc gttcgcagtt gattaatctg atgctggaat tacaggaaaa acagggtatt
    19621 tcgtatattt atgttaccca gcatatcgga atgatgaagc acattagcga ccaggtactg
    19681 gtgatgcatc agggcgaggt tgtcgagcga ggcagcaccg ccgatgtgct ggcatcgccg
    19741 ctgcatgaac tcaccaaacg gcttattgcc ggtcattttg gcgaggcatt gacggcggat
    19801 gcgtggcgta aggaccgtta gtggtttgca cctcatcctg acagggcccc cctcaggatg
    19861 aggtgttcat tacttttgat tgtcgattct ggtgagagca accagcaatg ccagcgtata
    19921 acacaccgta attgccagac tcatttttaa cagtgtttct ccgccaattc ccgacaatgg
    19981 cgcggctata ccgccgaaga caaacatgct catccccatc aacgcagacg ccgttccaga
    20041 ttcctgcgtc cctactgcac tcatagcctc tgccccgcca acagtgccga tgccgctaca
    20101 aaatgcgatg gtgaaaaata atgccaccag tgccggaatg ggcaattgta tccatgcgca
    20161 taagaccgtg agcaatgcac acaaaattgc cgctatcagg ccacctcgca acaatgtcat
    20221 cgcgttaatc cgtcgcgcca ggcgcgagaa gatccatgaa gcaatgataa gtccgatgcc
    20281 gttaaggcca aacaccaggc taaattgcat tggactaaag ccaaattcct tctgcaacac
    20341 gaacgacgaa gagccgatat atgcaaacaa accagccagc ataaaacttt gaatcaggca
    20401 aaagcgtata aaacggcggt tctgcaccac gcttcgtccg gttaatagca atgatgagcc
    20461 ccttttattt tctggcaagg tctcattaat aaataacagg cagcccagca acagtacggt
    20521 gctaatttca gccattaccc agaataaagt gcgccagtca aaagtgctaa caatgtaccc
    20581 gcccagcact ggcgacaaca ccggtgccag gccattcacc gtcattaaca gcgcaaaaaa
    20641 ctgggtcaac gttactccct gatatttgtc acgagcaata gaacgagaga gcaccgaacc
    20701 acccgccccc gcgatccctt gaataaaacg ccagaccacc agccagtaaa tattgttcgt
    20761 tgtcgcgcat aaaatggaag aaacaataaa caatagcaac gacaagatta acggacgttt
    20821 acgccctatt ttgtcgctca gagggccaaa taacagttgt ccgacgccaa gaccaatcag
    20881 tgatgcggtc agggttaatt gcgttatggt cgtggttgcc gccagttgct cagacatctc
    20941 cggcaaagcc ggcaaataaa gatcggtaca catcgggccg ataccggcta aaagaccaag
    21001 aatcaatgcc catgaaagag agactctagc catattattc cttacttgag tgatgacata
    21061 ccgccgccca gcgactgcca gagcgtgatt cggtttgtaa aatcagtcag ttgcagtgcc
    21121 agcagctcct gctgtgcgct ccacaagctg cgctgcgccg tcaacacggt caggtaatca
    21181 ccaacacccg cctgatagcg acgcaagcca acatcgaccg tttgttgctc agctttaaca
    21241 tactggcgct gtgcatccag ttgctcttca agcgtggtcc gccgcgccag tgcgttagcg
    21301 acatctttaa atgcgctctg aacggttttt tcataggtgg caatgagccc acgcttttgt
    21361 gcttctgcgt aacgaagctg cgccagatta cttccgtcag tgaacagcgg taatgtgacc
    21421 gatggcgcaa atgaccagat ctgcattccg tggctgaaca acgacgacaa tgcgtcactg
    21481 ccaacaccgg cactggcagt cagcgaaatc gtcgggaaaa agttagcccg cgccgcgcca
    21541 atatctgcat tggcgctctt caaattatgt tcagcttcct ggatatccgg gcgacgcagc
    21601 agaacatcag atgatactcc cgcaggaacc agacttatca tttgttccgg caggctttcc
    21661 agtgttcccg gtagcagatt ttccgcaagc gttgtacctg ccagtaagtt cagcgcattt
    21721 ttatcctgca tcacttgtgt ctgatagctg gcaacgctgg cgcgcgcttg ctgataaaca
    21781 ctcatcgcct cactgacatc tgtcgctgcc gccgtgccaa tctgttgctg gcgctggata
    21841 attttcaatg agttttctgc actggtcatc gtttcttttg ccagggccag gttgctgtta
    21901 tcagccgcca gtgtcagcca ggcggtgctg atttcagcaa tcagcgttaa acgagtgttc
    21961 tgggctgtaa attcactggc aagccaggtt tcctgcgccg cccgagataa actctgatta
    22021 cgaccaaaca gatctaacgt ataactggac accgtgccgt cagcctcggc agttgttccc
    22081 gtaccattcg ccagcgaacg actgcgagtc gaacttaagg ccgcattcac cgtcggaaat
    22141 aacgacgcat tagtttgccc atagagcgcc cgtgctgagt caatatcagc aatcgctttt
    22201 tgcacatcgc ggttactgtt cagtgcaatg gttactacct gctgcaatcg gggatcgtgg
    22261 atcacctgct gccagtcatg gctgattgcc ttcccctggc cctgtgctcc cggcaaggtc
    22321 gccgggattg gggattcagg cgtactgtag tggggatcga gcgagacaca tcccgcactc
    22381 aacaacacca gaaaaataag agaacgacgc aacataactt actcctgacg gcgcggttta
    22441 ccggcaaaca aacgtttcac cagtacaaaa aacagaggaa caaaaaaaat agccagtaat
    22501 gtagcggtca gcgtgccgcc aataatgccc gtaccaatgg cgatgcggct gttcgcccct
    22561 gcaccggttg ctatcgccag cggcattacc cccgcaataa acgccagcga ggtcatgatg
    22621 attgggcgta aacgagtctg cgccgcgcgt aatgccgcac gactcaagga atagccctct
    22681 gcaaccgccg cttcagcaaa ttccacaatc agaatggcgt ttttcgacga caagccgata
    22741 gtggttaata gcgccacctg gaagtaaaca tcgttgttta aatcacgcat ccaggccgcc
    22801 agcgccgcgc caagaagccc cagagggatc accagaatga ccgagaacgg gactgaccag
    22861 ctttcgtaca gtgctgcaag gcagaggaaa accaccagaa tagaaactgc atacagactc
    22921 aatgcctgac cgctggccag tttctcctgc aatgacaaac cactccaggc ccaggttgtt
    22981 cccgcaggaa gactgtttgc cagttcctcc atttttgtca ttgcatcgcc ggaactaaag
    23041 ccagtcgcat tttccccctg aatttcatag gctgcagaac cgttgtagcg caccagtctt
    23101 tccggtccat acagccagcg ggtggtcgca aaagcagaga atggtgtcat ggcgttatcg
    23161 ctaccgcgca caaaccattt acctaagtct gacggcgcgg aacgaaattc actgtcgccc
    23221 tgaatgtaga cttttttcac acgaccacgg tcaataaagt cattcacata agtaccgccc
    23281 cacgcactgg acagggtgtc ggtgacatca ttcaaactca accctaatga caccgcttta
    23341 ttactatcaa tatctacctg caattgcggc atttgtggca aatcattggc acgcacagaa
    23401 tgtaactccg gactttgatt cgctttttct atcaactgat tacgcatttg cagtagtgtt
    23461 tcgcgatcgg ttccaccgtt agctaacaat tcaaacgtaa aaccattgct ttggcccagc
    23521 ccatcaacgg ctggcggcgt catcgcaaat accgtggcat cacgaattgt gcccagctct
    23581 ttggtagccc gtagggcgat agcctgggcg gtgttttctg cccctttacg ttgagaccag
    23641 tttttcaacg aaacaaacgc catcccggtg ttctgtccgc tgccgctaaa actgaaacca
    23701 tcaacggtaa aaatgacatc ggtatttgct ttctcgttaa tcaggaacca gtcaacaatc
    23761 tggcgattca cttctgctgt acgggcagcg gtcgccccgg ccggcagcgt gtactgcacc
    23821 atgatttcgc cctggtcttc agtgggtaag aaactgcccg gcagtttcca catcatcagg
    23881 gccattccac cacccagtac gacataaagc cccatcgttc gagcagcacg gcgcaggaca
    23941 taaattacgc ctcgctgata tttatcttca gtacggcggt aaaagcggtt aaatgcgccg
    24001 aaaaagcctt ttttatgtgg cggaacatgc tggaggacgg aaccgcacag ggcgggagtc
    24061 aaggtcaatg ccaccaccac ggaaagcagc attgcggaga tgatggtgat ggagaactga
    24121 cgataaatta cgcccgtaga tcccccaaag aaggccatcg gcaggaatac ggctgacaac
    24181 accagcgcaa tggcaaccag tgcgccagaa atctcgccca ttgatttttc cgtggcttca
    24241 cgcgcgggca acccttcgtc acgcataatg cgttcgacgt tctctaccac cacaatggca
    24301 tcgtccacca gcaagccgat cgccagcacc attgcaaaca acgtcagggt attaatcgaa
    24361 tagccaaaca acgcaagaac gccgaatgtg cccagcaaaa cgacggggac agctaacgcc
    24421 ggaatcagtg tggcacgcag gttttgcagg aataaataca tcacgcaaac caccaatacg
    24481 atggcttcaa acagcgtctg aattacgtcc tctacggaga ttttaataaa ttcagtgctg
    24541 tctttcgggt atgcgatgtc gtatccctgc ggcatgttcc gctggaattc ggcaatctta
    24601 tccttgacca gcgtcgccgt attcagcgca ttcgcaccgg gcgaaagcat tacagcaacc
    24661 ccggcagacg ggtgaccgtt aagtttgccg atagcggtat aatcttcact gcccatctca
    24721 acgcgagcca catcttttat acgaacaact gcgccgtctg actggctttt cacgataata
    24781 tttttgaact gatccaccgt ctgcaaacga gactgcgcac gtaccgttgc ggtcagttgc
    24841 tgagtattcg gtgaaggcaa tgcccctatt ttcccggcag taacctgcac gttttgcgct
    24901 tcaatagcac tttgcacgtc tgaaggcatt agcgagtaag acgccagttt ggccgggtca
    24961 agccagatgc gcatagcgta ttccgcgcca aagacttgca gactccccac accttcaaca
    25021 cgcgccagcg ggtcctgaac gttactaacc aaccagtcgg cgatatccga actggaggct
    25081 ttgtcagtgg tgtcatacac ggcagcaatc agcaaaaagt tgctttgtga tttctccacc
    25141 gtaacacccg tttgctgcac ttcgctgggt aggcgcgact ccgcctgctg aattttattc
    25201 tgcacctgaa cctgtgcagt atctggatcg gtaccttgtt caaaggtcac attaatactg
    25261 accgaaccat cagagctgct ggttgagctg aaatagagta aattatcaag cccagtgagt
    25321 tgttgctcga taacctgagt cacgctgttc tccagcgttt cggcagaagc accagtataa
    25381 gtggctgaaa ttttaatggt cggtggcgca acgtcaggat attgcgcgac gggcaatgtg
    25441 cgaatagcca gaatccccgc caacataatc aaaatggcga taacccaggc aaagaccggg
    25501 cggcgaacaa aaaagcgcga aaacatcagg cgttgcctcc attagcctga acttcaacag
    25561 ccttgacggt ctgccctgaa gtgactttgg cagaaccttc aacaatcagt cggtcgccgt
    25621 tgtgcaggcc gttcagcacc agccatttat caccatacgt ttctcccgtt tcgagcgttc
    25681 gctgctctac tttattgtct ttattcacca ccagcgcagt tgcattgcct ttagcatcgc
    25741 gcgtgacgcc ctgttgcggc gcgagaatag cgtcttccat cacgccttca tcgacacgag
    25801 cgcgaacaaa cattcccggt aataactgct gttgtggatt gggaaaaatc gcccgtaatg
    25861 tcaccgaacc ggtagactca tctacagcga cttcggtgag ttccagacgc cctttttcgc
    25921 tgtaagttgt gccatcttcc agaataagtg agacgctcat ggtgtcactg ttggtcgcca
    25981 gtgactgttt acgcagacgt aataaatcga cactagagcg agtgaggtcg acatacattg
    26041 tatccagacc acgaatagtc gttaacgctg tatcctgcga cgcggtcacc agtgcgccag
    26101 gggttaccga cgaaatacca atacgccccg aaatcggtgc ggttaccgtg gtccagtcaa
    26161 gattaatgcg cgcagtttcc agtgcggctt ttttcgcctc tacactggct ttatcttgtg
    26221 cacaggtaga ctgagcatca tcagcatcct gttgtgaaac accgttctct ttcaccagtc
    26281 gggcataacg ctgcgctttc tggcaatcgg cttttaccag tgcctgtgct tgttgtaatg
    26341 ctgcccgagc ttcattccat gcagcctgat aactggccgc atcaatctgg tagagcggct
    26401 gtccagcctt gaccagatca ccttccttaa ataagcgttt ctggataatt cccccaacct
    26461 gcggacgtac ttcagcactg agcgcagcac tggtgcgtcc ggttaattca ctgactaccg
    26521 aaaccggttg agatttgagc gtaacaacgc cgacttctgt ttctgacggg gatgaattgt
    26581 tactttgcgt attatcacaa cccgataaga gcagcattgc cactacagat gttgctatat
    26641 acttcattat tttctccagg gtgagaaata ccctcctcat tgaaattaat gaggatatgt
    26701 cagggatatt cagttataac gaacccgcat cgcgaaatga atttcgcaat gaaataaggg
    26761 ggattaatat cccccgctat tggttaattc tgttcttatt tatttatcaa catactaaca
    26821 atatcctgat atatttcctg cagatgttgt ggatcgcttt tttgcggtgt taaacgacga
    26881 taaaccgtac cttccatcat ggtagcaaac acttcaacgc agcaacgaat gtgctcgtcg
    26941 ctaagatggg gatgcattct tttcatatgt gcacaaccgt tagcgaacat tctggcgtca
    27001 gcttcttcaa gcattgccat aacccgcggg ttgcgtgtgg cttctgcagc cacttccagc
    27061 atcagcgcgt cgtcgtcctc atttaaggat ttacgcaagg caaggacttc cgggaagtga
    27121 tcggtacgcg cgtcaatgtc catttgggca atacgaaagt cgatgatacg gcgaaccatc
    27181 tcttcaataa tggcgtcttt gttggcgaag taacgatata tctgacccac gctaagctga
    27241 gcttcgctgg caatttgcga catactggcg gcatgaaatc cgcagcgacg aaaacaccga
    27301 cgggcggcgt taatgatttc atcctgacgc tgtttgtggc gtatctccag cttgctggtc
    27361 atttctgtct cctggcctat cacttgatga gaatgatcgt tctcattttt gctaattata
    27421 cgtttctcca aagtaatgga aagtattttt atcttattca tgatgcatat tctgataatg
    27481 aagagattgt ggagattctg tgaagatagt tgacaatatt aagttggggt caattcatca
    27541 gaaagacagc gacttgttgt cgccagttct gttttcatca tcaaatttat cggagctaat
    27601 acgaatagtt tcatagtcag attctataag atatttcccc aacctccccc ttgcaggcgt
    27661 caggagcaga taacgcaaaa tgctctgtac cgcagcttct ctccgctaca gaaagcgcaa
    27721 ctatagccac ccacagccag gttgattata ataaccgttt atctgttcgt actgtttact
    27781 aaaacgacga atcgcctgat tttcaggcac aacaagcatc aacaataagg attaaagcta
    27841 tgggttttct ttccggtaag cgcattctgg taaccggtgt tgccagcaaa ctgtccatcg
    27901 cctacggtat cgctcaggcg atgcaccgcg aaggagctga actggcattc acctaccaga
    27961 acgacaaact gaaaggccgc gtagaagaat ttgccgctca attgggttct gacatcgttc
    28021 tgcagtgcga tgttgccgaa gataccagca tcgacaccat gttcgctgaa ctggggaaag
    28081 tttggccgaa atttgacggt ttcgtacact ctattggttt tgcacctggc gatcagctgg
    28141 atggtgacta tgttaacgcc gttactcgtg aaggcttcaa aattgcccac gacatcagct
    28201 cctacagctt cgttgcaatg gcaaaagctt gccgctccat gctgaatccg ggttctgccc
    28261 tgctgaccct ttcctacctt ggcgctgagc gcgctatccc gaactacaac gttatgggtc
    28321 tggcaaaagc gtctctggaa gcgaacgtgc gctatatggc gaacgcgatg ggtccggaag
    28381 gtgtgcgtgt taacgccatc tctgcgggtc cgatccgtac tctggcggct tccggtatca
    28441 aagacttccg caaaatgctg gctcattgcg aagccgttac cccgattcgc cgtaccgtta
    28501 ctattgaaga tgtgggtaac tccgcggcat tcctgtgctc cgatctctct gccggtatct
    28561 ccggtgaagt ggtccacgtt gacggcggtt tcagcatcgc tgcaatgaac gaactcgaac
    28621 tgaaataatc gttctgttgg tcaagacggg cggcgttctg ccgcccgtta tctctgttat
    28681 acctttctga tatttgttat cgccgatccg tctttctccc cttcccgcct tgcgtcagga
    28741 taacgatttc ctttacgacc aaggagcgcc catggaaaaa cgccacatta ccggcaaaag
    28801 ctactggtat catgaaacgc aatccagtac tgcggagtat gacgttctgc ctctggtccc
    28861 ggaagccgca aaggtcagcg atccctttct gctcgacgtg atccttgatg aagaaacgct
    28921 ggcccccttc ctttcatggc tggtccctgc gcgcgttctt gcagtggaat tgttccctga
    28981 ccagcttacc gtgacccgtt cacagacctt caccgcttat gaacgcttgt cgacggccct
    29041 gacggttgct caggtttgcg gcgtccaacg gttatgtaac tactattcgg cgcgacttac
    29101 gccgctcccc gggcctgatt cctccaggga aagtaatcat cggttagcac aaatcacgca
    29161 atatgcccgc caactggcta gctcgccttc tattatcgac aaccgatcgc gccagcatct
    29221 gaatgacgtc ggcctaactg cctgggactg tgtaatcatt aaccaaatca ttggttttat
    29281 tggctttcag gcgcggtcaa ttgcgacatt tcaggcttat ctcgggcatc cggtacgctg
    29341 gttacccggt ctggagatac aaaactatgc cgacgcgtca ctgtttgctg atgaatcaat
    29401 acgctggcga agcagctatg aggtggaaaa actccctgaa gagcacacaa aaagttcaac
    29461 tgcagaactt tgccaactgg ccgaaacact ctctctccac cctatttcac tttcccttct
    29521 cgaaaagttg ctaaacagca cacgggtcaa tacacagccg gataatcagc ttgcggcgtt
    29581 gttatgcgcg cggataaatg gcagtcctgc ttgttttgcc acctgtatga attcatcaaa
    29641 tgaatataaa aaaatcagcg cccttctgcg caagggcgaa aatgaaatta accaatgggc
    29701 tgaccgtcat tctgttgagc gcgctaccgt tcaggcgata caatggctga cccgagcacc
    29761 cgatcgcttt agcgccgccc agttcagccc attactcgaa cacgaaaaat catcaacgca
    29821 gattattaat ctgctggtat ggagcgggct gtgtggctgg ataaatcgct taaaaatcgc
    29881 gttgggtgag acatattaac cttgccgcgt cagacagatt cgcgtaaaac tgtcagccgc
    29941 tctaatggcc accaaaatag acaattatgt ttcaggacaa cccgctgcta gcgcagctta
    30001 aacagcaact gcattcccag acgccacgcg ctgaaggggt ggtaaaagcc acagaaaaag
    30061 gctttggctt cctggaagtc gacgcgcaaa aaagttattt cattccgccg ccgcagatga
    30121 aaaaagtcat gcatggcgac cgaattatcg cggttatcca cagtgaaaaa gaacgtgaat
    30181 ccgcagagcc agaagaactg gttgaaccgt ttctgactcg tttcgtgggt aaagttcagg
    30241 gcaaaaatga ccgtctggcc atcgttcctg atcatccact cttaaaagac gccattcctt
    30301 gccgcgcagc ccgtggcctg aaccacgagt ttaaagaagg cgactgggcg gttgccgaaa
    30361 tgcgccgtca tccgctgaaa ggcgatcgtt ctttctatgc tgaactgaca caatacatca
    30421 cttttggtga cgaccacttt gtaccgtggt gggttaccct tgcgcgccat aatctggaga
    30481 aagaagcacc agacggcgtc gctaccgaaa tgctcgatga aggtctggtt cgtgaagatc
    30541 tgaccgcgct ggattttgtc accatcgaca gtgccagcac agaagatatg gatgacgccc
    30601 ttttcgctaa ggcgttgccg gatgacaaac ttcagctgat tgtggcgatt gccgatccaa
    30661 ccgcgtggat tgctgaaggc agcaagctgg acaaagccgc gaaaattcgc gcattcacca
    30721 actatctgcc tggcttcaac atccctatgc tgcctcgcga gctttctgac gatctctgct
    30781 cactgcgcgc caatgaagtc cgcccggtac tggcatgccg catgacgctc tccgctgatg
    30841 gcaccattga agataatatc gaattctttg ccgccaccat cgaatccaaa gcgaagctgg
    30901 tgtatgacct ggtttctgac tggctggaaa ataccggtga ctggaagcct gaaagtgaag
    30961 caattgctga acaagtccgt ttgttagcgc aaatttgcca acgccgcggc gagtggcgtc
    31021 ataaccacgc actggtgttt aaagatcgcc cggattaccg ctttattctc ggtgaaaaag
    31081 gtgaagtgct ggatatcgtc gccgagcctc gtcgcattgc caaccgtatc gtcgaagaag
    31141 cgatgattgc cgctaacatt tgtgcggccc gcgtactgcg cgataagctc ggttttggca
    31201 tctataacgt gcatatgggc tttgatccgg cgaatgccga cgcgttggca gcgttgctga
    31261 aaacgcacgg tctgcatgtc gatgctgaag aagtgctcac gctggacggt ttctgcaaac
    31321 tgcgtcgtga actggatgcg caaccaactg gtttcctcga cagccgcatt cgtcgcttcc
    31381 agtcatttgc tgaaattagc actgaacccg gtcctcactt tggcctcggt ctggaggcat
    31441 acgccacctg gacttcgccg atccgtaaat atggcgacat gatcaaccac cgtctgctga
    31501 aagcggttat caaaggcgaa actgcgacgc gtccgcagga tgaaatcact gtccaaatgg
    31561 ccgagcgtcg ccgtctcaac cgaatggcag aacgtgatgt tggcgactgg ttatacgcac
    31621 gcttcctgaa agacaaagcc gggaccgaca cccgtttcgc ggcggaaatt gtcgatatca
    31681 gccgtggcgg catgcgtgtg cgtttggttg ataacggcgc tatcgccttt attccggcac
    31741 ctttcttaca cgctgtgcgc gatgaactgg tttgcagcca ggaaaacggc actgtacaaa
    31801 ttaaaggtga aacggcttac aaagtgactg acgttattga cgtcaccatt gccgaagtcc
    31861 gcatggaaac ccgcagcatt attgcgcgcc cggtcgcgta atctcctttc acggcccatt
    31921 ccttatgaat gggccgttta tttccccgct ctaccttcat catatcccgg cagtttttta
    31981 actgactttc gtttgaaaac tggcgttttt ccaaaactgg attacaaata aatatataaa
    32041 aaaaacaatg cgtttagccg ccgttaattt catgtaagtc atgttgttcc ttttatttaa
    32101 ccgcggactc cgctgttaac cggaggatat gcatcatgaa aaccgttagg gagtccacaa
    32161 cgttgtacaa ctttctcgga tcgcacaatc catactggcg gttaacggaa agcagcgatg
    32221 ttttgcgctt ttctaccacc gaaaccacag aacctgaacg tacgttgcag ttatctgccg
    32281 aacaggctgc tcgcatcagg gaaatgacgg tcatcacctc cagcctgatg atgagtctga
    32341 ccgtcgatga aagcgatctt tctgtgcatc tggtaggacg aaaaatcaat aaacgggaat
    32401 gggctggcaa cgcgtctgca tggcatgaca caccagcagt tgctcgtgat ttatcacacg
    32461 gactttcctt tgctgagcag gtagtttctg aagcacattc cgcaatagtg attctcgaca
    32521 gccgggggaa tatccaacgc ttcaatcggt tatgtgaaga ttacacaggg ttgaaagaac
    32581 atgacgtcat tgggcaaagc gtgtttaaac tgtttatgag ccgtcgtgaa gctgcggcat
    32641 ccaggcgcaa taaccgtgta ttttttcgaa gcggcaatgc atatgaagtc gaactgtgga
    32701 taccaacacg taaaggccag cggctgtttc tgtttcgcaa taaatttgtc cacagcggca
    32761 gtggcaagaa cgaaattttt ttaatctgtt ccggcaccga cattaccgaa gagcgccgcg
    32821 ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg ccgaatcgta
    32881 acgcaatgca ggagttaatc gatcacgcca ttaatcaggc agataacaat aaagttgggg
    32941 ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcttatggg catttgtttg
    33001 gtgaccagct attacgcgac gtgtcattgg ccattttaag ctgtctcgaa catgatcagg
    33061 tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc tcacaaagcg
    33121 cgctggaagc aatggcatca cgaattttga cccgcttacg actccccttc cgcattggtt
    33181 taattgaagt ttataccagc tgttcagtgg gtatttcact ctctcccgaa catggttcgg
    33241 acagcgcggc tattattcgt cacgccgaca cagcaatgta cacagcgaag gaaggcggac
    33301 gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa tatctctggc
    33361 tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac tatcaaccga
    33421 aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg cagtcacctg
    33481 aacgtggatt gattccaccg ttggacttca tttcctacgc cgaagagtca gggctaattg
    33541 tgcctttagg ccgttgggtg attctcgatg tagtacgcca ggtggcaaag tggcgggata
    33601 aaggtattaa cctgcgagtg gcggtaaaca tttctgcacg tcagctcgcc gatcaaacca
    33661 ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc cctattgatg
    33721 ttgaactgac ggagagctgc ctgattgaga atgatgagct ggcactgtct gttattcaac
    33781 aatttagcca actaggtgcg caagtgcatc tggacgattt tggtaccggt tactcttcac
    33841 tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt tttgttcgag
    33901 atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct gtggcccagg
    33961 cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat gcttttttaa
    34021 ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg cccgccgtcg
    34081 ccttcgaacg ttggtataaa cgctatctga agcgcgca
//
LOCUS       DAZKGF010000028        33834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZKGF010000028 DAZKGF010000000
VERSION     DAZKGF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.028"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..433)
                     /locus_tag="ACHOQV_001370"
     CDS             complement(<1..433)
                     /locus_tag="ACHOQV_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAT RNA binding domain-containing protein"
                     /protein_id="HFP9629767.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDVEYIFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSV"
     gene            complement(691..1944)
                     /gene="amiC"
                     /locus_tag="ACHOQV_001371"
     CDS             complement(691..1944)
                     /gene="amiC"
                     /locus_tag="ACHOQV_001371"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFP9629768.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2176..3507
                     /gene="argA"
                     /locus_tag="ACHOQV_001372"
     CDS             2176..3507
                     /gene="argA"
                     /locus_tag="ACHOQV_001372"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFP9629769.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3745..5571)
                     /gene="recD"
                     /locus_tag="ACHOQV_001373"
     CDS             complement(3745..5571)
                     /gene="recD"
                     /locus_tag="ACHOQV_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFP9629770.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPSGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5571..9113)
                     /gene="recB"
                     /locus_tag="ACHOQV_001374"
     CDS             complement(5571..9113)
                     /gene="recB"
                     /locus_tag="ACHOQV_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFP9629771.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKLLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALMLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDAITAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVDEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDSLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGITLEEA"
     gene            complement(9106..11994)
                     /gene="ptrA"
                     /locus_tag="ACHOQV_001375"
     CDS             complement(9106..11994)
                     /gene="ptrA"
                     /locus_tag="ACHOQV_001375"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFP9629772.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12170..15538)
                     /gene="recC"
                     /locus_tag="ACHOQV_001376"
     CDS             complement(12170..15538)
                     /gene="recC"
                     /locus_tag="ACHOQV_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFP9629773.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWEIGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDADRLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIARRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15551..15874)
                     /gene="ppdC"
                     /locus_tag="ACHOQV_001377"
     CDS             complement(15551..15874)
                     /gene="ppdC"
                     /locus_tag="ACHOQV_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFP9629774.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15859..16266)
                     /gene="ygdB"
                     /locus_tag="ACHOQV_001378"
     CDS             complement(15859..16266)
                     /gene="ygdB"
                     /locus_tag="ACHOQV_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFP9629775.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16263..16826)
                     /gene="ppdB"
                     /locus_tag="ACHOQV_001379"
     CDS             complement(16263..16826)
                     /gene="ppdB"
                     /locus_tag="ACHOQV_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP9629776.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(16817..17287)
                     /gene="ppdA"
                     /locus_tag="ACHOQV_001380"
     CDS             complement(16817..17287)
                     /gene="ppdA"
                     /locus_tag="ACHOQV_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP9629777.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17472..18266)
                     /gene="thyA"
                     /locus_tag="ACHOQV_001381"
     CDS             complement(17472..18266)
                     /gene="thyA"
                     /locus_tag="ACHOQV_001381"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9629778.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18273..19148)
                     /gene="lgt"
                     /locus_tag="ACHOQV_001382"
     CDS             complement(18273..19148)
                     /gene="lgt"
                     /locus_tag="ACHOQV_001382"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9629779.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19299..21545)
                     /gene="ptsP"
                     /locus_tag="ACHOQV_001383"
     CDS             complement(19299..21545)
                     /gene="ptsP"
                     /locus_tag="ACHOQV_001383"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9629780.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21558..22088)
                     /gene="rppH"
                     /locus_tag="ACHOQV_001384"
     CDS             complement(21558..22088)
                     /gene="rppH"
                     /locus_tag="ACHOQV_001384"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFP9629781.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22441..22587)
                     /gene="ygdT"
                     /locus_tag="ACHOQV_001385"
     CDS             complement(22441..22587)
                     /gene="ygdT"
                     /locus_tag="ACHOQV_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFP9629782.1"
                     /translation="MLSTESWDNCEKPSLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22773..23462
                     /gene="mutH"
                     /locus_tag="ACHOQV_001386"
     CDS             22773..23462
                     /gene="mutH"
                     /locus_tag="ACHOQV_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFP9629783.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23531..24244
                     /gene="ygdQ"
                     /locus_tag="ACHOQV_001387"
     CDS             23531..24244
                     /gene="ygdQ"
                     /locus_tag="ACHOQV_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP9629784.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24382..24600
                     /gene="ygdR"
                     /locus_tag="ACHOQV_001388"
     CDS             24382..24600
                     /gene="ygdR"
                     /locus_tag="ACHOQV_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFP9629785.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24708..25748
                     /gene="tas"
                     /locus_tag="ACHOQV_001389"
     CDS             24708..25748
                     /gene="tas"
                     /locus_tag="ACHOQV_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFP9629786.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25780..26973)
                     /gene="lplT"
                     /locus_tag="ACHOQV_001390"
     CDS             complement(25780..26973)
                     /gene="lplT"
                     /locus_tag="ACHOQV_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFP9629787.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLSAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRY"
     gene            complement(26966..29125)
                     /gene="aas"
                     /locus_tag="ACHOQV_001391"
     CDS             complement(26966..29125)
                     /gene="aas"
                     /locus_tag="ACHOQV_001391"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFP9629788.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29711..30742
                     /gene="galR"
                     /locus_tag="ACHOQV_001392"
     CDS             29711..30742
                     /gene="galR"
                     /locus_tag="ACHOQV_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFP9629789.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30749..32011)
                     /gene="lysA"
                     /locus_tag="ACHOQV_001393"
     CDS             complement(30749..32011)
                     /gene="lysA"
                     /locus_tag="ACHOQV_001393"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP9629790.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAARQQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            32133..33068
                     /gene="lysR"
                     /locus_tag="ACHOQV_001394"
     CDS             32133..33068
                     /gene="lysR"
                     /locus_tag="ACHOQV_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFP9629791.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(33055..33747)
                     /gene="ygeA"
                     /locus_tag="ACHOQV_001395"
     CDS             complement(33055..33747)
                     /gene="ygeA"
                     /locus_tag="ACHOQV_001395"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFP9629792.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAIIRAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADKRAKINQI
                     IFEELCLGQFTEASRTYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
BASE COUNT         8282 a         9216 c         8385 g         7951 t
ORIGIN      
        1 gaacgctgaa ttgctcatta atgatagtta atgcatcgcg acttatcgca tactctgctt
       61 tgtacaacgc tttgatttca tcaaggaaaa cattattaat ttgtatacct gaaactactc
      121 gggatattgc attcgaaatg tgatccgcaa gaatcaatag tagcgatgga ttgagatttt
      181 tttcgagatt tttctccgca tattgcacta ttttttcggc aacaaaaata tactcaacat
      241 ccacatgttc aatcaatttg tagagtttat ttttttgttc gtttctgaca taaaaaatcc
      301 ggttagccgg atgttcaggg accgacattc catatttttt gttgtaaccg acgccaggtc
      361 cagaaatgat aacctcctgt ccattcatcg atgcctgtac acaattattg ttcatgactt
      421 tttcgatgat cattccatga ctccaaaaaa aaaggcaagg cacgtcagaa gtaataatta
      481 cttcaaacat tgccttgcct gatttaacag ttacaagatg attaactaat gcgtatacat
      541 tacacggaat tgtgcttaat taaagagatg gtcggcttat ttttcaagcc gacattcaaa
      601 tattcgacat cagtcacaaa accagcatct aatattcatg aaaatgttat ctattcaacg
      661 aaatataaat tcattgaata aataaaaaca tcatcccctt ctcgccagcg tcgccccatc
      721 ggcaaaatac gctttaatcc ccgcaagaat agactccgca acttcctgct ggaaagtcgc
      781 cgttttcagt ttacgttctt cctcaacgtt actgataaac gccgtttcga ccagaatgga
      841 gggaatatct ggtgccttta gtacggcaaa cccggcctgt tcaacttgat ttttatgcag
      901 cttgttgatt ttaccgagct tattcagcac cgctttacca aacttcaggc tgtcggcaat
      961 ggtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac cgcttttgct
     1021 cacgccacca atcaagtccg aggcgttctg ggtttgtgcc agatattttg ccgcagtact
     1081 ggttgcacct ttggttgaga gcgcaaacac agaggaaccg ctcggctgac gactggtaaa
     1141 ggcgtcggca tggatagaga caaacaggtc agcacgctgt ttctgggctt ttgctacgcg
     1201 cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca tattgccctc
     1261 tttctcgatc agagagcgca gacggcgagc tatttgcaat accacgtctt tttcgcgcgt
     1321 tttgtatttc cccaccgcac cggagtcttc gccaccgtgg ccagggtcaa gcataatgac
     1381 aataggacga tcgcgccctg ctttacccgg ttgtggacca ctttgtgctg gcggcacctg
     1441 cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt cctgcatatc
     1501 ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg cgaccggcgc
     1561 aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatac gtacggtttg
     1621 cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtcagcgc ggatttgcgc
     1681 agccatcccc ttgagtaccg agttcaggtt tacatcttcg atatccacca ccacgcgttc
     1741 aggattactc aacgcgaact gcttatattt cagctgacga tttgattcta ccgtcacgcg
     1801 ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctgaccg cagccaggct
     1861 gacctgactt acgctcaata gccacatggc acccgcgcct tgcagtaaac gacgacggct
     1921 gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa gctgtgtaac
     1981 aagtaaacga ccaatttgac cggaaacttt agcgaaagac gcataatctg tcatctataa
     2041 aacggtaaac attcttttta tattcacggt attactgata aaaaactcgc tcccgcataa
     2101 aatttacact tgcaccctgc gaaaaaacag aataaaaata caataatttc gaataatcat
     2161 gcaaagaggt gtaccgtggt aaaggaacgt aaaaccgagc tggtcgaggg attccgccat
     2221 tcggttccct atatcaatac ccaccgggga aaaacatttg tcatcatgct cggcggtgaa
     2281 gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttgtt gcacagcctc
     2341 ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa tctggctgcg
     2401 catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa aacactggaa
     2461 ctggtgaagc aggctgcggg aacattgcaa ctggatatta ctgctcgcct gtcgatgagt
     2521 ctcaataaca cgccgctaca gggcgcgcat atcaacgtcg tcagtggcaa ttttattatc
     2581 gcccagccgc tgggcgtcga tgacggcgtg gattactgcc atagcgggcg tatccggcgg
     2641 attgatgaag acgcgatcca tcgtcaactg gacagcggtg caatagtgct aatggggccg
     2701 gtcgctgttt cagtcactgg cgagagcttt aatctgacct cggaagagat tgccactcaa
     2761 ctggccatca aactgaaagc tgaaaagatg attggttttt gctcttccca gggcgtcact
     2821 aatgacgacg gtgatattgt ctccgaactt ttccctaacg aagcgcaagc acgggtagaa
     2881 gcccaggaag agaaaggtga ttacaactcc ggtacggtgc gctttttgcg tggcgcagtg
     2941 aaagcctgcc gcagcggcgt gcgtcgctgt catttaatca gttatcagga agatggcgcg
     3001 ctattgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat ggaaagcgcc
     3061 gagcagattc gtcgcgcaac aatcaacgat attggcggta ttctggagtt gattcgcccg
     3121 ctggagcagc aaggtattct ggtacgccgt tctcgcgagc agctagagat ggaaatcgac
     3181 aaattcacca ttattcagcg cgataacacg actattgcct gcgccgcgct ctatccgttc
     3241 ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta ccgcagctca
     3301 tcaaggggcg aggttctgct ggaacgcatt gccgctcagg cgaagcagag cggcttaagc
     3361 aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg atttaccccg
     3421 gtggatattg atttactccc cgagagcaaa aagcagttgt acaactacca gcgtaaatcc
     3481 aaagtgttga tggcggattt ggggtaagtg gtggaaatcg ccagatgcga catgcgcaat
     3541 gcctggaggc atgataaaca cgctagcatc gcatcaggta ttgacgctca tcgtcggatg
     3601 cggcgtgaac gccttatccg acctacatca aatcaaaggt gttgatggcg gatttagggt
     3661 aagcgacgga aatcgccaga tgcgcatatg cgcaatgccc ggaggcatga caggacgcgc
     3721 atgcgtcgca tcaggcaatc acgtttattc ccgcgaacta aacaatgccg ccagaccact
     3781 gcgccgctca gtgcgcgtgg caattgccgc acttaaaatg cgctcatcgg catacagcga
     3841 cagacggcga cgcgcgcggg tcactgcggt gtaaaccagc tctcgcgtta ctaccggcgt
     3901 gcgttgactc ggtaaaatca acgccgcatg gtcgaactcc gatccctgcg atttatgtac
     3961 cgtcatcgcc cacgtcgttt cgtgctctgg caggcgactc ggttgcacag acttaatatt
     4021 gccgtccggc atcgcaaacc aaacgcgcgt cccctgcccg cgatccagcg caataccgat
     4081 atcgccatta aacaacccaa gcgcgctgtc attacgggca atcatcaccg gtcggccttc
     4141 gtaccaacga gagtgcggat tacgatgaat tttgcgcttc tgttgcataa actgctcaat
     4201 tcgctcattc agtccagcca cgccaaacgg cccttcccgc agggcgcaca aaagctggta
     4261 ctcattgaac gcctgaatga ttaaatccgg ctcggcacgc gcttgcagca gatccagata
     4321 acgcccgtaa cccgcaagag cctcctcaag catcgcaata tagtcttcgc cgctctgtaa
     4381 gagccgtttt tcgatatcag taaaatcctg ctgaaaaacg gttttcactg ccgttttatc
     4441 accacggttg atcgccgcag ctaactgacc aatgccagaa tcgctgccga aacgatagct
     4501 tttttgcagc aggcagagac tgtcgcgcaa agatgccgct tctgtgccag ttcctgacgg
     4561 aacgtgagtt cccgtcaggc ggctcaactg cccggcacgc tcggcggtaa agcccgcgtt
     4621 ggcataagcg cagatatcgc ccagcacagc tccagcctca actgaggcca gttgatcgcg
     4681 atcaccgagg aagatcactc gcgcatgatc gggcaacgca tcgatcaggc gcgacatcat
     4741 ggggagatcg atcattgacg cttcatccac caccagcaca tcaagatgca gcgggttacc
     4801 ggcatgatga cgtaaacgct ggctacccgg ctgcgcaccc agcaatcggt gcaaagtgct
     4861 ggcatcttcc ggaatgcgtt tcttttgttc atcggtcagc ggtaactggc gcaaagcctt
     4921 gccgagagat tcggttaagc gcgcggcagc tttacccgtt ggtgcagcca gacggatacg
     4981 gcagcgttcg ccgtctgcca tttgaattaa cgctgccagc aacttcgcta cggtggtcgt
     5041 tttaccggtg ccagggccgc cggaaatcac cgagatccgc cgcgtcagcg ccactgccgc
     5101 cgccactttt tgccagttaa tttcatcgct tactggaaaa agtttgtcca gggtttgcgc
     5161 cagtagagct tcatcaacct caatggcatg attcacttcg ttgaaaaagc gtgccactgt
     5221 gcgctcgtta caccacatgc gattcaaata aagacgatca ccacagagga tcatcggcgt
     5281 tggttcatct ccccggctga ccgcttgtga agccagcaag cattcttccc aattttgtaa
     5341 ctcaccgatt tcgctgacgc aggttgctaa cagcggatgt gacgcctcgt tattttccag
     5401 tcgtgagagc ggtaaacaga catgcccttc cccggcgtcg tggcttaaca gggctgccgc
     5461 gagggtgacg gcaggatgtt catctcccgc cacggtcagg gcaaattgca catccagcgg
     5521 gcgtaactgt ttgtgctcca cagcttccag taattgcttt tgcaatttca ttacgcctcc
     5581 tccagggtga taccggcaaa catctcatcc atcaggtcaa tcaacccggc gttgggtcgg
     5641 gtcgcgtaga tcccttgttg cggatgttct ttatcaacac cacgcaggaa cagataaata
     5701 acgccgccaa agtggcgctc atagtcgtaa tcagcaatgc gatggcgcag ataacggtgc
     5761 agcgccaggg tataaagctg atattgcaga tcatagcggt gtgcctgcat tgccgctgcc
     5821 atagcctgtt gggtgtaagc agaactgtct tcacccaacc agttggattt atagtcgagc
     5881 aggtaataac gcccttcgtg gcggaacacc aggtcaataa agccttttaa catgccgcgt
     5941 acctgcatga actccagcgg cgggcagcca gcggatagcg agtcaaactg gcgaattagt
     6001 gcatcaagct ggctggcgat gagcggttcg ctaatcggca ggtaaaactc catctccacc
     6061 tgtttatcgc ggtcggaaag ctgactcagg ctaacacccg tttcattgag aggtgcctgg
     6121 aggacagccg tgatccactc ggtcaacacc ggttcccact gcgattcaaa gccgccgagt
     6181 tccagttttt cctgtaccca gttcgggtca accggctggg taaaatcgag gtcttcaaac
     6241 aaactgtgca agaatgtccc cggtgacgca ccgcgcggga actgatgcgg tgttaacgtc
     6301 ggttcgtcaa cgacgctgac cacgcctgcg gcatcaacat ccagccgtgg catcagatcc
     6361 tgagcgatac cgtgaccacg ctgctgcaaa ccggagtagc tggtgacacg ccagttatcg
     6421 ccgggcaatc gttgtagcgt cctcgcattc agttctgcag ttatagcatc attaacctgc
     6481 cagggctggt tatcaccagt ttgtgccgtt tgccaggcaa tatcatcatc gcataacgct
     6541 tcaatacagg tgcgaagccc tgccgcatct tgcggttccc ctttttgcag caaacgcccg
     6601 agcgcacttt ggtggacgtc ggtgtcacct tttttatcgc cacgacggcg caccagcggt
     6661 gcaacgccga gactgcaatg ccaaaccgaa cgcgtcagcg ccacgtaaag caaacgcaga
     6721 tcttccgcca gacgttcggc ctccgcgaga tcgacgcttt ctggcgcagc attaagatcc
     6781 agaacagcct caaacgagtg gcgatcgtga tagaacgcct gatcctggac gcggaaattg
     6841 gtaataaatg gcaaccagac cagcggatat tccagccctt ttgatttatg gatcgtgaca
     6901 atctgcacca gatgtttatc actttcgagg cgcatttgtt ggctggaggc attactgtct
     6961 ggctcgagga tatgttgcga taaccagcgt accagcgcat gttcactttc cagctgcgtt
     7021 ccggcttctt gtagcaattc gctgatatgc aagatatcgg taagacgccg ctcaccgcct
     7081 gctgttgcca gcaagttttc tgcaatgtta cgcgccgaca tcagcgcccg cagcatcggc
     7141 ataacgccac gtttgcgcca gatttgccga taaccatcga actcttcgac gaccgcatcc
     7201 cacgcatgtt cgtcattatt cagcgtttca atatccagcg cattcagccc catcattgac
     7261 gttgccagcg cactgcgcag ggtgttctca cgttcgggag tcatcaccgc ctgcaacagc
     7321 caaagcatct cctgcgcttc cagagtttca aaaacactgt cgcggttcga aaggtaaacg
     7381 gaagggattt ccagcagcat taaggcatcg cgcacctggg cggcctcctg gcggctgcgc
     7441 accagcacac tgatgtccga agcacgcacc ggacgcgcgt cgtcgccgtt catcagcaac
     7501 gcttcgcccc gctgtccggc ttgtagccag tcgcggattt gcgcagcaca tacctgcgcc
     7561 atggtacttt gatagtcgcc aacgccgcag ctttcgcctt ccatcagcca cattttcatc
     7621 gcaggctgcg tttcaccttt aaacacaaaa cgtaacgcct gatttttgcc ggcagatttc
     7681 acaggaataa acggtatttc acgaaacatg aacgcgtcat cagtctggct gaaaagtttg
     7741 ttaacgctgt tcaccattcc cggtgcggaa cgccagttgg tatctaaggt gtagtgagca
     7801 tgaacttcgc tacgcgcctt catataagtg aagatatccg caccccggaa tgcataaata
     7861 gcctgtttcg ggtcgccaat cagcaacaat gcggtttccg gctgatggtg ccagatacgg
     7921 cgaaaaattc gatactgctg ggggtcggta tcctgaaatt catcgatcat tgccaccggg
     7981 aatcgcgtac ggatcgccgc tgccaatacc tcgccgcttt cactacgcag cgcggaatcg
     8041 agccgactta acatgtcatc aaaacccaat tcgccacggc ggcgtttttc acgcgctact
     8101 gtttcgcgga tctcagccaa tgcgcgggtg atcaccagat cgcggatcga caatggttct
     8161 gcaagcagtt gatcgatcgc ctcaaacagt ggatgtcgcg gggtttcccc cccggctttc
     8221 gtgcgatctt ctaagaaacg ctgagagaat ttttccagcg actccggcaa ctgatagctg
     8281 ttggtctctt cttctgccca ggcgctgatc ttgtcgatcc atttagcctg attgctacgg
     8341 ttaaacttgc gtcgatcaat accagaagat tcgatcagcg catccagttc acccactgcg
     8401 tcgcgccact gctgttttac ggcatcaata cgcgccacaa tttgcgcgtg gcgggaagca
     8461 agcgtttcat catcgggcgg cggtgctttg ataaccggtg cttcgccttg cagataacga
     8521 ttaatatcgc gcagcaacgc ctgcggccct ttccaggttt caaagaccac ctgggcaatt
     8581 tcacgcggca gcgggtagca gtggcgacgc cagaaatcgg cgcaggcctg gtagcgtagc
     8641 agagactcat cttcaatcag ctgctgctca aacagcatgc cggattcaaa ggcattcagg
     8701 ttgagcatgc gctggcaaaa accgtgaata gtaaagactg ccgcttcatc catctgccgt
     8761 tcggctaaca acaaccactg cgcggcttgc gctttatcgt cgatctcctc cagcaggagt
     8821 ttgtacagtg gattgtcggt ggtttcacgc agacaggcga tgcgcaactc gtggatattg
     8881 ctacggatac gaccgcgcaa ttctgccgtg gcagcctcgg taaaggtgac caccagcagt
     8941 tcttcaacgg tcagcgggcg gggaaaggcg gcagaaccgc ctagtccaag taacaggcgc
     9001 aaatagagcg ccgcaatcgt aaaggttttg cctgtgccgg cagaggcttc aatcaggcgc
     9061 tcgccctgta agggcaagcg caaaggatct agtgtctcgg cgacatcact cattcttttc
     9121 actcatcagg ggcattgttt gctgcaacgc gctgacgttc tcccacactt tccagccttc
     9181 aggatgcaca tattcggctt tcccgttctg gctgccggaa atctgcgaca gaatagccat
     9241 gccttgcggc tcgaccaccg cctgatggaa gaaatcagca agtttttgcg gcgtcagcag
     9301 ttttatctgg gccacgattt tatcacgcga atcgaagcgc atattgccgc gatcgaaatc
     9361 tttacttaac ttcgatgctt cttcgccgag cgtttgcggt gcctgcagca tctgggttat
     9421 taccgcctgc tggatttgcg caaactcctc tggcttcatc gcccgcaatt ttgcctctgc
     9481 ggttgggaaa aacgccttgt aacgctccca caagaatgaa ggctgtttat cattgctttg
     9541 caaaaggaag cccatgcccc actgacgccc cacgctcatt ggaaacgcaa acacggcata
     9601 gcccaactgt tcttcggtac gcaactgatt gtagaaccac ggctgtacga tctgccccaa
     9661 cagagagcta taggctgagc tggtgtattc atcgtagcca gtcggtacaa ataccgctgc
     9721 cagtgcggag tcggtgctgt taccggcttt ttcaaagatg acagattgtt ttttatcgac
     9781 cacgacatct ttgttacggc accactccga accatcagcg cccaactgtt tttgcacatc
     9841 gcgtgccagc gttgttgcct gggcttcggt catgttgcca ataaccataa actctggtcg
     9901 agcccctgat tttaaggcgt cgcgataggc cagcacctct ttcaaagtga ttgaaggcaa
     9961 aattttgcgc cgttcatctc gcgagaagta cggcacttgc gagagcatct gcgcgggcat
    10021 aatcgcctgc tcgaacgctt tgcccttttc tgcggaatcc atcatctggt tataccagga
    10081 cttcgcctgc tcaagctgat cttccgtagc ggtatagcta aagtacccct cgagcaatgc
    10141 ctggaacagc tgcggcagac gctgggtgta accattagca ttaaccataa ggccgttgtt
    10201 agcgttggtg gaaaaactaa tgccaccaac cgacgcctgg ttgcttaact gatcaagcgc
    10261 tagcccggca agataatcat tgagcgcaaa catcacctga ttgcgggcgc tgtccatggc
    10321 tttcggatta cgcaaaatca ggctgacatc agctttgggt tcgctggaaa aataacggct
    10381 tggcgcatag accacacgca gattcgactc atcaacaata agctctggat ggtcatattt
    10441 cttctctgac ttaatcagcg agaaatcgtc aggaatatag gggttaagct ctggcaaaga
    10501 gagcgcaatg tcggcggctt ttttctgcca gtcagcgaaa gtttgtgcgc taattttatc
    10561 gacctgatac ggcgcatcga caaagtaagc cgttttgttg tgcggctctt tcgggctgat
    10621 ataccagata cgcgcattct gcggcgtcat catcgccagg cgttctttta ctgctttagc
    10681 gtcgtaccga tcggcaatat tgactgcatc cagcgtatgc tcaacaggaa cgcgaatcat
    10741 ggtatctgcc agccattcga cgtaatccat atcacgggtg attgacggat aacgaaagtc
    10801 gatatccagc acattcgcca gttcatcgaa gtattgttta tcaatccctt tttcgcgtaa
    10861 cagattgagg tagctaaaaa tggccgccac aacctgatcg cgattcgcca gacctttatc
    10921 ggttaaagac gcagagatcg ctaatacgcc gctgttgccg ttgacgatag gatcggaatt
    10981 ggcgctaatg ccctcaacta atccctgctt ttgcagccag tcagaaagtg tacctgggct
    11041 gcgattaccg atcagatagg taatcaattc atccgtttta ctacggaact tcgctgaatt
    11101 gttatcgatg cgaaactcaa cgcgcagaac tttacgcggc agcgccggga cgtaatgaat
    11161 gataatacct ttttgcgcgt cggtgactac cggcacggtg atttccggtt ttttgctctc
    11221 tttgttcggc acgcgaccaa aggtgtccgc cgccattttt gccaactccg gcagcggttt
    11281 attgctgtaa ataaccgcct tcatcagatt ggcagagtaa tatttctcgt gaaaatcttt
    11341 cagcgcctgc tgcaccggat ttccgggttt gtcgcttaaa gtttcgaggt taccaccaga
    11401 aaactttgaa ccggggtgtg ccgggttaat ggtttctgcg ctgacctgtg ccatgcgcat
    11461 cccgtcacgc gtacgcgcca tggttaattc ggcattcact gcattacgtt cacgttcggc
    11521 gtatttcttg tcgagcaagg gttctgcaat agcgtccgcc aggcggtcta ccgcaccggg
    11581 caaggcgtcg ttctcaactt ccagatagaa agccgtgcga tacggtgccg tgctggcatt
    11641 gtgactgccg ccgtgcattt tgagatattc ggccagactg tcagcctgcg ggtacttttt
    11701 cgaccccatc agactcatat gttcaaggta atgtgccagc ccctggtacg cctcgggatc
    11761 ttccagcgac ccaacgggca ctaccagcgc cgagagcgat ttaaccgcct gcggatcaga
    11821 aaccagcaag accaccatac cgttatccag acgtatagcc tgatactggc ggttatcttt
    11881 atcactttta cggatggttt cctgaatcgg ctgccatccc gtttctgcct gacttaaggg
    11941 tgcccaaagg gcaacaaaca acaataatgc tttgaaccag gtgctgcggg gcattcacgg
    12001 acctcataat caactttatt ttctggccag attcaacagg ttaacgcaaa tcatctgcca
    12061 gaatttaatc ttgtgctgaa cgagtcagcc tatgtctata tatccatcag tccgtgactg
    12121 gtgcgcatca taaagcaagc agatatattg cgcaattttt atacagcact catgactgat
    12181 taaagcgaaa cagcggtaac aggaaacgtt gcgactgttc aacgatagcc tccattgtct
    12241 ctggtgttaa ttgccgccag agccgttgat accagatatc atcaccttcg ccacgcacca
    12301 tcatgttgcc ttcgtaagcc tgaaggaatt tcgtacgggc tttttgtaac gtggaatcgt
    12361 catccagcat ggcatcgttt tgcgcgtcat aacaggtttt tagccacgcg ccgccacttt
    12421 caggtaacac cagcaatggc gcggacattc cttcacgata cccctcaatc agttgtgaga
    12481 ggtaatgcaa agcctgttcg gctgcaagcg gcggaaaacg ccactcgccg tctttgcgta
    12541 gaaaaaggcg actttcacca ttaccaccgc tggcacagta gacaaggtgt tccagccaaa
    12601 gttgcattcc ctgcgccaca cttaataaag agggacgcca gcgcaacagg ccatccggct
    12661 gcacctgcgg caaccagcca gttatctgca caccgttgca ggcgagatca atttccatac
    12721 tctgccccgg ctggcgacgt gcaatgactc tgtcggcaag ctgctgcatc tcctggcact
    12781 gtgtttccca gaaaatttca ccaaaagcgc catacggtaa atcccctgcc gcccggaagc
    12841 ggcggaataa gcgatcggca tcatcctgct caaccagtgc attcaataac tgctgattga
    12901 tttgataacg gctaagccct tccagaataa atggctcggt gtcggggatt tcgctgtctt
    12961 cagtacggaa gttcacctgc aaacgcatct ggaagaacgc ccgtaccgga tgtgcccaga
    13021 atcgttgtag cgtttccagc ggcacggttt ccggtaaggt aaacggcagc ggctgaacaa
    13081 attcagaatg tgctttacca gcctggctgg ccgcaggtag ccattcacga gcatagcttt
    13141 gtcgttcgcc tggctggtag ttttgcggat caaacggcat ccgggtatgg aggcaagtaa
    13201 gatgcgcttt tacccttgcc tcgctttcat cacagttgag cgcttcatcg cccggtagat
    13261 aatggctttg cccgatgtag tcgatcagtt cctgcaccag taccgacggg aaacgttcac
    13321 tgttatcctg aatagaacgc ccgatatagc tgatatagag tttttgctgc gcggaaatta
    13381 acgcttccag gaacagatag cggtcgtcat cgcgacggct acggtcgcca cgcttcggtt
    13441 tctggctcat caggtcaaag cccaatggcg caagctggcg tggataaacg ccgtcgttca
    13501 ttcccagcag gcaaaccact ttgaatggga tggaacgcat tggcatcaga gtacaaatgt
    13561 taaccggtcc ggcgagaaaa cgctggctga tacgttcctg atccaggcgc tgtgccagtt
    13621 catcacgcaa tagtgacagc ggcaccgcgt cgccatactg cgcacctaaa ccttcggcga
    13681 taatcgcctg ccattgttgt tcgatcagcg tcatagccgc ttcggtttcc gcatccggca
    13741 ggaagaaggc gttgagcata tcgcgacaaa ccggcaacca ctcttccagc ggacgctcct
    13801 gcgccagccc gcgacgccag atgtttagct gcattagcag tgaagccaga tgccccacca
    13861 gttctgcaat taagccgctc gattcatcat aaggtagaac cgattgccac tcgccctgcg
    13921 cgctctccat cgcgtagccc agcaacatac gcgtcaggcc aaatcgccag gtgtgttgtc
    13981 cggtggcggg gagttccagc tcgcgaacgt tgtcgtcatc tatcccccaa cgaatgccgg
    14041 attcgttgac ccactggcgt aaataacgca gcccttcttc ggtgatgtca aaccgcgccg
    14101 ccagcaccgg cacatccagc agcgccagca catcctctga cacaaagcga ctgtcaggca
    14161 gtgataacag gctgataaac gcttccaata ccggatgtga ctgccgcgcc cgacggtcgg
    14221 aaatggcgta aggcaggtaa cgatccgcag gtgcgctacc aaacacagcc tgaataaacg
    14281 gactgtagct gtcgatgtcg gccaccatca cgatgatgtc gcgcggagtc agtgtcgggt
    14341 cttcctccag catcgccagc aggcgatcgt gtaaaacttc aacttcacgc tgcgggctat
    14401 ggcaaacgtg aaaggtgata ctgctatcca gtggatcaag cgggcgtttg ttatcgctac
    14461 gggaaaactc ttcgatgttt acaccagcaa cggcgcggtt ttccagttcc agaatgtcag
    14521 actgaatgtt atgcagcagg ttatctggcg taacatcgac aaaagcgtcc agctcctggc
    14581 tgctctccag atcagaaagg agataaatgt agtcgcgccc aagcttgccc catgaggcca
    14641 gcagcgggtt gccgacatcc tgttcaccat cgctgttaaa gagctgcccg gcattttcgc
    14701 tgtcgcgaaa tagcggtaat tctcgatctt caaaactgtg tcggcgctgg cgagtcagta
    14761 gtttcgccag ataagcagga tctttaatgt cgccccagta ataacggcag gggttggtaa
    14821 acaggagatg gatttcaata tgtttaccca gcgcctggag tgcctggaga taaacaggcg
    14881 gtaacgcgga aataccgcat ataaagacgc gcgaaggtaa ccccggcggg caggtcgtcg
    14941 cggactccag cgtttcgata aagcgctgat agagattggc gcggtgccag cgcggttgcc
    15001 cgagttcatg ggtatattcc accaacgcct tccacaacgg cgcttgccag gcctgtgctt
    15061 ctcccagccc ttcaaccaaa tgtcctattt cccactgtgc cagccagtcc ggacgataga
    15121 ccagatactg gtcaaacagg tccgccgctt ttgaagaaag ctggaacagt tttcgcttgt
    15181 cgctatcgtc agtcagataa tgccgcaaca gggtaaagtc ttcgcgctcc aacagttgcg
    15241 gcagcagagt catcagtttc cagctcatgc tctgtttgtt aaaggcgctc tctttgggga
    15301 tttccggtaa cacccggacg aacatatccc agataaagct cgctggcagc ggaaaatcaa
    15361 tgtttgccgc aataccaaac ttttgcgaca gggtcatttg cagccactgc gccataccgg
    15421 tactttgcac cagaatcatt tctggttcga aaggatcgtc cagccgttcg cgttcgacga
    15481 taaactccat caacgcttcc agcacgtcca gacgattgga atggtagacc cttaacatag
    15541 cggctcctga ctactgacga ttcgggcaat gcaggcgggt catctcgcct tctctgcccc
    15601 cgggtgaaac tagcgtaacg ctgatgctga cacatcccgc ctgcgatgtc tgcattcggt
    15661 tgacctgcca gttagcaggt ggcgaaatcg cgcgcagttg cgtttgctgc cagccatgcc
    15721 gccagagctg ttggtactgg tttctgctgg caagactgtt cattaatgtt cgctggaaac
    15781 ccgataacgc agtgacaatc atcaccatca acaccatcgc caacattacc tccggcagac
    15841 taaagccttg ttgattcctc agggaagctg acataacgcc ctctctttca gcggacaaaa
    15901 atcgctccag ccgcgtggcg aaaaaacaat gtttccatcg atgacttcgc ctgttcgcca
    15961 caacgaaacg ccttcataac cagcaatcaa taaggcttca ttatctgcca gtaaacgcag
    16021 acaaacccgg gcaccggtgg cagcatacag taagcactga actgcgggca gcgtctgcca
    16081 ggagtgcatt tttccccact ccagcgccga ctgaacgatg gcctggcggc gcaatgactg
    16141 actttccatg ctcacgcgag aagcaaaact gcgatcctgc tgactcattc cttgcaatag
    16201 caagctaccc aaaatcagca gcatcaggac cagagccagt gacgaaacac ccttttcgcg
    16261 gttcacaggt tggatcctgt cacgctatag ctggcatcca ccacggtttg cggatcagcc
    16321 ttgctggcag cgtgcatatt aaccgtcaac accggcgaga agccgctgac atcctgacgt
    16381 acgacctgaa aagtgtcgat aatgatggca tccggattag tgactttatc ccagccctta
    16441 ccttcacagg atgtcgcacc gcgtagcgtt tccagcacat gctccttcag acgaaatcca
    16501 atctggtcgg actcttttac cggttcgcga tcccagatac cattactgtt cgcatcccac
    16561 tgcacaatga cacagtcacc ctgtccgaca atttccagcc cttcgcccgt acagctgcca
    16621 tggcaataac ccgccctctg gagatgcttc gcgacggtaa atacccgcag ccagatttca
    16681 tcttccagcg ccagcttacg ggtgttcgtt aaactttcac gctgtaacgc aggcagaaag
    16741 cgtgcagccc ccagcaacaa tacgctactg atcgccatag caatcaacac ttccagcaga
    16801 gaaaaacctt gctcttttac aggcatcctt ctgtttctcc ttgctgacaa agccggagtc
    16861 ttccccacgg cgaaaccact agccaccact cgcccgttga gtttttgaag cgaatatgcc
    16921 cggcccatgc ggtattgcgc aggccaaaga aagcaagcga aggtgtcagg tcgctcattt
    16981 cgacttcggg ccagcgcggc acaaagacca atggtgaact gccatgacag gtattcgccc
    17041 cagcaacgga actcacaagg caccataacg tcccctccct gataagactg atactgtggt
    17101 cgcggttatg ccagttggca tcttcacgta aatagagcaa atagtcccgc gcctggctgg
    17161 cggtttgcca aagccgttgc gactgctgcc agtattgcca gccataaagc ccacttgcgc
    17221 ttagcatgac cagaatcagc atcgcgacca gcgtttcaat cagcgtataa ccacgttgtg
    17281 ttttcatgcc ggcagtatgg agcgaggaga aaaaaagacg agggccagtt tctatttctt
    17341 cggcacatct tccggactat ttacgccgtt gcaggacgtt gcaaaatttc gggaaggtgt
    17401 ctcgaagaat ttaacggagg gcaaaaaaaa ccgacgcaca ctggcgtcgg ctctggcagg
    17461 atgtttcgta attagatagc caccggcgct ttaatgcccg gatgtggatc gtatccttca
    17521 atctcaaagt cttcgaaacg gtagtcgaag atggattcgg gtttacgttt gataatcaac
    17581 ttcggcagcg gacgcggttc gcggcttaat tgcagatgag tttgatccat atggttgctg
    17641 tagagatgcg tgtcgccacc ggtccagaca aaatcaccca cttccagatc gcactgctgc
    17701 gccatcatat gcaccagtaa cgcgtagctg gcaatgttga acggcaggcc gaggaagacg
    17761 tcacaggagc gctgataaag ctggcaagag agtttgccgt ctgccacata gaactggaag
    17821 aatgcatggc acggtgccag cgccatttta tccagttcgc ctacgttcca cgctgaaaca
    17881 ataatgcggc gggaatccgg gtcgtttttc agctggttca gtacagtagt gatctggtca
    17941 atatgacgac catccggcgt tggccaggcg cgccactgtt taccatacac tggcccgagg
    18001 tcgccgtttt catcggccca ttcgtcccag atggtgacat tgttttcgtg tagataagca
    18061 atgttagtgt cgccctgcag gaaccacagc agttcgtgga tgatggaacg caggtggcaa
    18121 cgtttagttg tcaccagcgg gaatccatcc tgcaggttaa aacgcatctg atgaccaaaa
    18181 atggaaagcg ttccggttcc ggtacggtcg tttttctgtg tgccttcgtc gagcactttt
    18241 tgcatcagtt ctaaatactg tttcatggtt cctcaggaaa cgtgttgctg tgggctgcga
    18301 cgatatgccc agaccatcat gatcacaccc gcgacaatca tcgggatgga aagaatttgc
    18361 cccatgctga tgtactgcac ccaggcacca gtaaactgcg cgtcgggctg gcggaaaaac
    18421 tcaacaatga tgcgaaacgc gccgtaacca atcaggaaca agcctgagac agctcccatt
    18481 gggcgcggtt tacgaatata caggttgagg ataataaaca gcaccacacc ttccagcagc
    18541 agctcgtaaa gctgtgatgg gtggcgcggc agcacaccgt aagtgtcgaa aatggattgc
    18601 cactgcggat tggtttgcag cagcaaaata tcttctgtac gggagccagg gaacagcatg
    18661 gcaaacggga agttcgggtc aacgcggccc cacaattcac cgttaataaa gttgcccagg
    18721 cgcccggcac caagaccaaa cggaatgagt ggggcaataa aatcagagac ctggaagaag
    18781 gaacgtttag tacggcgggc gaagataatc atcaccacga taacgccaat caggcctccg
    18841 tggaaggaca tgccgccgtc ccatacacgg aaaagataca gcggatcggc cataaactgc
    18901 gggaaattgt agaacagaac ataaccaata cgtccaccga ggaagacgcc gaggaagccc
    18961 gcatagagta agttttcaac ttcatttttg gtccagccac tgcccggacg attcgcccgt
    19021 cgtgttgcca gccacattgc aaaaatgaaa cccaccagat acatcaggcc gtaccagtga
    19081 agcgccacgg gtcctattga gaaaatgacc ggatcaaact ccggaaaatg cagatagcta
    19141 ctggtcatct gtcaccacaa gttcttgtta tttcgctgaa agagaacagc gattgaaatg
    19201 cacgccgcag gtttcaggcg ctccaaaggt gcgaataata gcacaagggg acctggctgg
    19261 ttgccggata ccgttaaaaa atatgtatat gatccgcgct ataacccccc gcgaatcaac
    19321 ccgcccatgc cgcgacgctc cataaaggct gcaacctgat ggcgaacttc ggtcgccagt
    19381 tgcgcttcca gactacgctg cgcaagattt tctgcttcag caaaatcaat gcgccgcagc
    19441 aggtatttta cgcgcgctac agaacgtccg ttcatagaca gatggcgata cccaagccca
    19501 atgaggattg ccacgcacat gggatcgccc gccatttcac cgcacaaacg gagatcgatt
    19561 ccatgtattt ccgcttcccg ggcgatcatc gccagagctc gtaacattgc aggatgaaga
    19621 ctgtcataaa tgttcgccac ccgggtattg ttgcgatcaa cggccaggat gtattgagtc
    19681 agatcgttgg tgccaacaga gatgaaatcg acccgctttg ccagatgcgg cagcataaat
    19741 accattgacg gcacttccag catgatgccg atacgtggtt tgggaatttc gtaaccgatc
    19801 atctcctcga cttcacgtcc ggcacgttca atcaggcggc gtgcttcgtc aacttcatcg
    19861 aggcttgtga ccatcggcaa cagaatattc aggttgcccg tagcggcatt agcacgcagc
    19921 atcgcccgca cctggatcaa gaagatctcc ggctgatcga gcgtaatgcg aatcccacgc
    19981 caacccaggc atggattctc ttcgctaatc ggcatgtaag gcagctgctt atctgctccg
    20041 acatccagcg tacgcaaggt gacgggttta tcattaaaca tttgcagcat cccctgatac
    20101 tgcgccacct gttcttcttc cgacggaaaa ccactttgca gcatgaatgg gatttcagtg
    20161 cgataaagtc ctatgccatc aatacggctg cccagttttt cttcatgttc cgggcttaaa
    20221 ccggcattga gcatgacttt aatgcgctcg ccgcttttta actgggcggg taaattgacg
    20281 tcatcttccg ccagacggct aagctcgatc tcttcactaa ttagccgctg atattcttgc
    20341 agcagtaccg gttccggatc gaccagcaat tcaccgcgat aaccatcgac gatcagcgtc
    20401 cgacgatgca gtaccgaagg ctgaatatcc gcgcccatca cggtaggtat ccccagcgca
    20461 cgtaccatga tcgcagcatg ggagttggca gcgccatcgc gcacgacaac accgactaag
    20521 cgatcctggg gcagctcagc aagcgttgtc gctgacagtt catctgccac cagaatgaaa
    20581 cgttccggcc aggcgttcgg cccttgatta gcgtcatcaa gatgaaacag caatcgctga
    20641 cccagcgcac gtaaatcgcc agcccgctct ttgagatagt tatcgcttag cgcggcaaac
    20701 tgttcggcaa atttttcaat gaccgttttt accgcccact ctgccaccga gcctttatca
    20761 acctcggcaa acaattcgcg acgcagccgg gtatccgaaa gcaggtgtga gtaaagatcg
    20821 aaaatagccg ccgtttcttt ttgtgcaccg gcagcaaagc gtttgctata gcggcgaaac
    20881 tcgttagccg cctcttccaa cgccccggtc agtcgttcgc gttccagagc cggatccagc
    20941 gttgatgcct gatacacctg ttccattaaa ggtaacgtgg catcctgcca gccttcggca
    21001 atcgccacac caggtgctgc cggtaatgca cggattcgcg tctggcgata ttgcccaaac
    21061 aaggcagtca actgcgactg agaaagaata gctgccatct gggtggcaag cgtcaccagg
    21121 aaggattctt cactttcgtc atactggcgc aactctcgtt gctgtaccac cagtacacca
    21181 agcaactggc gacgttgaat aattggtacg cctaaaaacg cgcggaaacg ttcttctttt
    21241 acggagggga tgtatttgaa gctggggtgc ttttgcgcat ctgcaaggtt tatcggttcc
    21301 gccagcctgc caaccaggcc gacgatccct tcatcaaacg cgagcgttac agtgcgaccg
    21361 cgtggttttt tcagtccccg ggtcgccatc aggtagtaac aacgtcgatc atgatcggcc
    21421 aggtagaccg aacagacctc ggtatccatc gcaagacaga tgtcggtaac cagaatattt
    21481 aacgcctcat tcaggcgtgg tgcgctggct accttttcga ctatttcgcg caggcgagtg
    21541 agcataattg gcgtgactta acctctttta cgtcgataag cagatgcgtt ttgtggtttt
    21601 ggcgtatttt cctgcagtga catcaccaca ctcgcgaact ctttcattac cctacggtag
    21661 acatcacgtt taaatgacac cacctgtctg actggatacc agtaacttac ccatcgccag
    21721 ccgtcaaact ctggtgtact gctggtttgc atattgattt ctgcatcgcc gctcaccagc
    21781 tgcaagagaa accatttttg tttttggccg atacaaaccg gcttcgtgtc ccaacgcacc
    21841 aaacgtttcg gtaatttgta gcgcaaccag ttacgcgttg aagcaaggat tcgaacgtct
    21901 ttgcggctta atcctacttc ttcaaacaat tcacggtaca tcgcctgctc tgcggattct
    21961 ccggggttga ttccgccttg cggaaattgc caggagtgct gaccaaatcg ccgggcccac
    22021 attacctgcc cctggcgatt acaaatcacg atacctacgt ttgggcggta gccatcgtca
    22081 tcaatcaccg gactacctca aaataaagct ttatatacga atgattgttt catactccag
    22141 gaagacggta aaccactctc tgcagggcat tacacactaa taacaattga ataactcaca
    22201 gttatgtgca gagttataaa cagaggaagg ggtggatagc cgtttttatt caccttttct
    22261 gtggatagag ttgtgaagaa ctacggaatt actacgggaa aacccggagc attctgaata
    22321 agacagatac agagatttta aaatattcat tatcagtcaa tgcattacat gtgatttgag
    22381 taataaaaaa gagtaattcg tgacccaggt cacacctctc atttacgggt tggcgaaaga
    22441 tcaaccaatg ccgtatttat ccacagaatg tgccactaag ttaagcactg aaccactaaa
    22501 aactggagtt tcgtcgcacg tcaaggctgt aaatggaaac agtagtgaag gtttttcaca
    22561 gttatcccag ctttctgtgg ataacatggt gtaagatcct gtttattttc agtgaccaga
    22621 tttggaaaac ccgttgcagt gttgcgcaac tcgattaccg gcaacctaaa aagcaatata
    22681 aatcagagaa ttgaacaacg catgtggaaa aagttacact gcgaatattc ggcacttaat
    22741 tgctgtttgt tttttaacca aggtatcatg acatgtccca acctcgccca ctgctctctc
    22801 ctcccgaaac tgaagaacaa ttgttagcgc aagcacaaca actttctggt tatacattgg
    22861 gagaactggc ggcacttgcc gggctggtta cgccggagaa tttaaaacgc gataagggct
    22921 ggattggcgt gttactggag atctggctag gtgccagcgc agggagtaaa cctgagcaag
    22981 attttgctgc tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc
    23041 tggaaacaac attcgtttgt gttgccccgt taacgggcaa tagcggggtg acctgggaaa
    23101 ccagccacgt gcgccacaag ctcaaacgcg tgctgtggat accggttgaa ggcgagcgca
    23161 gcatcccgct ggcaaagcgt cgcgtaggat caccgttgct gtggagcccg aatgaagagg
    23221 aagaccgaca gttacgcgaa gactgggaag aattaatgga tatgattgtt ctcggtcaga
    23281 ttgagcggat caccgctcgt cacggggaat atttacagat acgaccgaaa gcagcgaatg
    23341 cgaaagcgct gaccgaagct attggtgccc ggggcgaacg gattctgacg ctgccgcgcg
    23401 gattttattt gaagaagaat ttcaccagtg cgctactggc ccgtcatttt ctgatccagt
    23461 agccatcgct ttgacctgcc gctttccggg catataatta ccgcttcatt tttttggcag
    23521 ggctttttag atgttatttg catggataac cgatcctaac gcctggcttg cgctcggtac
    23581 actgacgctg ctggagatcg ttcttgggat cgacaatatt attttccttt ctctggtggt
    23641 ggcaaagctt cccacagcac aacgtgctca tgcgcgccgt ctggggttgg cgggagccat
    23701 ggtcatgcgt ctggcgctgc tggcatccat cgcctgggtt acgcgcctga cgaatccgct
    23761 ttttacaata ttcagtcagg aaatttccgc ccgtgatttg atcctgcttc tgggtggctt
    23821 gttccttatc tggaaagcca gcaaggaaat ccacgaatcc atcgaaggtg aagaagaagg
    23881 gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg cagattatgc tgctggatat
    23941 tatcttcagc ctcgactcgg tgattaccgc tgtgggtctg tcagatcacc tgtttattat
    24001 gatggctgcc gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga
    24061 ttttgtcgag cgccatcctt cggtaaaaat gctggcgctc tctttcctga ttctggtggg
    24121 ctttaccctg attctggaaa gtttcgacat ccacgtaccg aaaggttaca tctacttcgc
    24181 gatgttcttc tctattgcgg ttgaaagcct caacctgatt cgcaacaaaa agaatccgct
    24241 ctgatactcc gtacgctctc ctgcgggagg gcgtactcac tcctgttacc ctcacagttt
    24301 aagattttcc tgctttcaag atatataacg tcggtttata aacagactat tatcataggt
    24361 gggccaaatg aggataaccg gatgaaaaaa tgggcagtaa taatttctgc agtcggactg
    24421 gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg
    24481 attttgaccg atggaaaacc tgaaattgat gatgataccg ggctggtgag ttatcacgat
    24541 cagcaaggta acgcgatgca aattaaccgt gatgatgttt cgcaaattat tgaacgttaa
    24601 caaataaggt cagcatccgg ctggccttaa gatttttctc tttccctttt cccttccctc
    24661 tgccattttt atattcctta tgtcgtgatt ataaaaagga aacggctatg caatatcacc
    24721 gtatacccca cagttcgctg gaagtcagca cgctggggct tggcacgatg acgtttggtg
    24781 aacagaacag cgaagccgac gcccacgcac aactcgacta tgccgtcgct cagggcatta
    24841 accttatcga cgttgccgaa atgtacccag tacctccgcg ccccgaaact caagggctaa
    24901 ccgaaaccta cgtcggcaac tggctggcga aacatggcag ccgcgaaaag ttaattatcg
    24961 cctccaaagt gagcggaccg tcgcgcaata atgacaaagg catccgcccg gatcaggcgc
    25021 tggatcggaa gaatatccgc gaagcgctgc atgacagcct caagcgtctg cagactgatt
    25081 acctcgatct ttatcaggtg cactggccgc agcgcccaac caactgcttc ggcaaactcg
    25141 gttatagctg gacagattct gcgcctgcgg tttcgctgct ggatacactg gacgcactgg
    25201 cagagtacca acgcgcggga aaaattcgtt atatcggcgt gtcgaacgaa actgcatttg
    25261 gcgtaatgcg ctacctgcat ctggcggaca aacacgatct gccgcgtatt gtcaccattc
    25321 agaaccccta cagtctgtta aaccgcagtt ttgaagtagg tctggcagaa gtcagccagt
    25381 atgaaggggt cgaactgctg gcctattcgt gcctgggttt cggcacgctg accgggaaat
    25441 atctcaacgg tgcaaaaccc gctggcgcac gtaatacgct ctttagtcgg ttcacccgct
    25501 atagcggtga gcaaacgcaa aaagccgtcg cggcgtatgt tgatatagcc agacgtcatg
    25561 gcctggatcc tgcacagatg gcgctcgctt ttgtacgccg tcaaccgttt gttgccagca
    25621 ctctgctggg cgcaaccacg atggagcagc tgaaaactaa cgtcgaaagt ttgcatctgg
    25681 agttaagcga agacgtatta gcagaaattg aagcggtgca tcaggtttac acttatccgg
    25741 caccataaga ttaacgccgg ttaaaaccgg cgttaaatat taataacggc gctgccagat
    25801 ccacagcgcc gttattgcca gcgcaaacag cgcaccgaag ccaatgccaa tgggcacgac
    25861 cgggatgcct accattaccg ccagcgagta aatgcccagc atcaacaaca tggcgctgtt
    25921 ttcgccaagg ttttgtactg caatcgcatt ccccgccccg acgctttttt taccccgttc
    25981 ctgtagcaac gcattgagcg gaacgacaaa aaagcccccc agcacgccaa tcagcatcaa
    26041 caaggcatag gctgacagca gctcgtgttg cagggagaaa atcagtacca ccacgccaat
    26101 caaaatcccg gctggcatac agcgtgacac ggtttccagc gtaactaact tcgctgccgc
    26161 accagcgcca accacgatac caatcgctac catcgcgttg agataggtcg gcgtagcgtt
    26221 atcggtaatg cccagcgcca ccggtaccca cagcaccaac aggaaacgca gcgtgacacc
    26281 cgctccccag aataaactgg tgcccaccag cgaaaaacgc gtttcaccat tgcgccatag
    26341 cgaggtgcag gcattcagga aactgcgggt catgctgatg agattccagg actgccccgg
    26401 acgcgccgcc gccagtttag gaatgtagat attggcaacg accgcaccac cgtaggccag
    26461 tgcgcatgcg gccagggcga ccaggacatg ccagtcagcc agtacaccac cggctacgga
    26521 accgagcaaa atcgccgcta tggtagaagc ttccattaaa ccattagctt tcactaactt
    26581 actacccgtg gttaattcgc cgagaatacc gtatttcgcc ggtgaatagg ctgcagcacc
    26641 aacacccacc agcgtatagc cgagaaacgg attgatacca aagcagatac tggctgcgcc
    26701 cagcagcttc aggccgttgg caaacatcat cacccggcct ttggcgaagc tatccgccac
    26761 ctgtccgaca aacggcgcaa aaagaatgta agcacctaca aacaccattt gcaggatggg
    26821 ctggctccac tccggataaa actgcgcttt cagtaacgcc agagtggcaa acagcagcgc
    26881 attatcgcca aacgcagaga gaaactgcgc cacgataacc gctttcatcc ccttcgacca
    26941 caacgaagtg ttagtgtgca ctgactcact catcgtgttg ttccgcttcg tctacccagc
    27001 ttttcaacgt gacaaagtca ggtttgccgc tgccaagtaa tggcatctgt ttcagatagc
    27061 gaatatcgcg cggtacagca agctccggca cgccgtgctc gcgggcatac tgttgcaact
    27121 tatcgcgcgt cagttcgtta tctgtggtga aaagcaccag tgcctcgcct ttgctggcat
    27181 cgctcttaat cgcagtggca tggactttat ctggcgaaac accaagtgcc agttgttcca
    27241 ccatttccag cgacaccatt tcgcctgcaa ttttggcaaa gcgttttgcg cggccctgaa
    27301 tctgcacaaa gccctgctcg tcgaaacgca caatatcgcc agtgtcatac cagccgcgct
    27361 ccatttcgcc gcgaacattc tcggcggtgg gcacttccag tacacctggc ttctccaccc
    27421 gcagatagcc gttcattatg ttcggccctt tcagttgcag gcgtccgccc tcttcgatac
    27481 caggaaccga caacaggcgc gcatccattc ctggtagaat acgccctacc gtaccgggtt
    27541 tcgccgccat cggtacgttg atagaaacga caggcgcgca ctcggtcaca ccgtagcctt
    27601 caaggatgcg caggccaaat ttatcctgcc aaagctgttt ggtactttct tgtagttttt
    27661 ctgcgcctgc caccacatag cgtagacggt agaagtcata cgggttggcg aaacgcgcgt
    27721 agtgaccgag aaaagtcgag gtaccgaaca acacggtgca actgcggtca tacaccaact
    27781 ccggcacaat gcggtaatgc agcgggcttg gataaaggaa cacctctgca cctgtgagca
    27841 gtggcgtaaa caggcctacc gtcagcccaa aggagtgaaa cagcggtaac gccgacataa
    27901 agcgatcgtt ggtggtgaag tcggcaatcg ttttaatctg ctcgacattc gccagaatgc
    27961 ttttatggct atggacgacg cctttcggat ggccttcaga accggaggta aaaaggatca
    28021 gcgcctcttc ttccggctgc tgttttacct gtgccagacg cggcatcagc aaatgagcga
    28081 agatccatac tttgtcggca gtggtgacat ctgcttttaa atcttccaga tagacccagc
    28141 gcacctgagt aagttgctcc ggcagatgcc agagtttgcc tttatcgaga aactgacggg
    28201 aagtgaaaat ggttttgatt tcagccgccg taatagcact ggtcaaccct tttaccccgg
    28261 cggtgtagtt catcattgcg ggaatgcggc gacgggcgat ggccccaaaa atcactgccg
    28321 cactgatgcc tgcattgggc agcattaagc cgatgcgttc gccttcaaca ctgtattttt
    28381 caaggatgcg tccaacaaac agcgttttcg taagcaattt gcgataggag tccggggtaa
    28441 agttaacgtc ttcgacacat ttcttcccgg ctccgaagcg gtacattgcg ctcagtaaag
    28501 attcgtacag cgtttcacgc gggcgcaccg ccatacgcgc ttccatcatt atttgatgca
    28561 gcatttcgcc agcgattttg cgacggtcac gggcacgcgg cgcatccggc atctccacct
    28621 gcgttggtgg caaaatatgc agagtaattt gcgggaacaa gcgacgttta accagacctt
    28681 tcaggcggct gaagtgcgta agttccgccc cttcaatacg cacaggaata accgttgcac
    28741 cagacttcgc cgcgacaaat cccgcgccat cgtagatttt catcagcgag ccagtggtgg
    28801 tgatgcgtcc ttcagggaaa atcaccactg gtcggccctg ttccaccaga cgtaccagat
    28861 gtttaatcgc cataggttgc gtcgggtcga gaggaacaaa gtcgataaat gatttcagcc
    28921 aacgcatata ccactgttgg cttattgagg tgtaaacggc aaataccgga cgtacgggta
    28981 aaaacaatgc cagcagtatg ccatcaataa aagagacgtg attaggcgtg attagaacgc
    29041 gctcgccctt cagtgcctgg gtgtcacccg taacgcgaac gcgatacaaa acacggcaca
    29101 aatttcgaaa aaagctaaaa agcattccaa ctccctttgc tctgattcag taaaagcgaa
    29161 tggagggaga ttacacgaga taaagaacgc gagcgacagt aaattaggtg cgaaaaaaaa
    29221 cctgcgcatc cgcgcaggtt ggtgcaagag acagggtacg aagagcgtac cgaataatct
    29281 caccaatcaa tacctctggg atcttgattg tggtctgcac gacgactctt cgccagtgag
    29341 aaaacgcaaa ggaatgaagg gaaatgcaac gaggtgtgta aattgtcggt tactgttaca
    29401 gattgatgac cggcaaaaaa aaacctgcgc atctgcgcag gctggtgtaa ttcatgtgct
    29461 caacccgaag ttgacttcac ctatcaatac ctctgggatc accactttag caacctgaag
    29521 ccaaacgcca ccagcggtca atcgcaacag cgtttcgcaa agtgtaacca aaggtttgca
    29581 atctcttttt ttgtcttact tatttgcgtt tggctcacat tcccacaatg aaaacacgcc
    29641 gccccttgaa ccaacgggcg ttttccgtaa cactgaaaga atgtaagcgt ttacccacta
    29701 aggtattttc atggcgacca taaaggatgt agcccgactg gcaggcgttt cagtcgccac
    29761 cgtttcccgc gtcattaata attcacccaa agccagcgaa gcttcccggc ttgctgtgca
    29821 tagtgcaatg gagtctctta gctatcaccc gaacgccaac gcccgtgcgc tggcgcagca
    29881 gaccactgaa acggtcggtc tggtcgttgg tgatgtttcc gatccgtttt tcggtgcaat
    29941 ggtgaaagcg gtcgaacagg tggcttatca cactggtaat tttttattga ttggcaacgg
    30001 ttaccacaac gaacaaaaag agcgtcaggc cattgagcaa ctgatccgcc atcgctgtgc
    30061 tgcgttggtc gtccatgcca aaatgatccc ggatgccgat ttagcctcat taatgaaaca
    30121 aatgcccggt atggtgctga tcaaccgtat cctgcctggc tttgaaaacc gttgtattgc
    30181 tctggacgat cgttacggtg cctggctggc tacgcgtcat ttaattcagc aaggtcatac
    30241 ccgcattggt tatctgtgct ctaaccactc tatttctgac gccgaagatc gtctgcaagg
    30301 gtattacgat gcccttgctg aaagtggtat tccggccaat gaccggctgg tgacatttgg
    30361 cgaaccagac gaaagcggcg gcgaacaggc aatgaccgag cttttgggac gaggcagaaa
    30421 tttcactgcg gtagcctgtt ataacgattc aatggcggcg ggcgcgatgg gcgtgctcaa
    30481 tgataatggt attgatgtac cgggtgagat ttcgttaatt ggctttgatg atgtgctggt
    30541 gtcacgctat gtgcgcccgc gtctgaccac cgtgcgttac ccaatcgtga cgatggcgac
    30601 ccaggctgcc gaactggctt tggcgctggc ggataatcgc cctctcccgg aaatcactaa
    30661 tgtctttagt ccgacgctgg tacgtcgcca ttcagtgtca actccgtcgc tggaggcaag
    30721 tcatcatgca accagcgact aaccgcagtt aaagcaattc cagcgccagt aattcttcaa
    30781 tggtctggcg acggcgaatc aaccgcgcct gaccattatc aaacagaact tctggtaaca
    30841 gcggacggct gttgtagttg gatgacattg atgcaccata tgcccctgta tcatgcagta
    30901 ccagataatc acctgccttc acttccggca aggcgcgggt ttcgacattt cccccttcct
    30961 gctgggtaaa gacatcgccc gattcacata acggcccggc gacgacggtt tccaccgttg
    31021 gtgcgtgttc cagagaacga ccatcagctg ccagggcact gatatggtgg taactaccgt
    31081 acattgccgg gcgcatcaga tcgttgaacc cggcatcaac cagcacaaag tggcggctac
    31141 ccatttgttt gacgctccgc acttgagtaa ttaatacgcc agactgcgct accagaaagc
    31201 gacccggttc aatttccagt ttcacagggt ggcccaaatg gcgggcgatt tgctgacgcg
    31261 cggcattcca cagaccataa taatgctcgg tatcaaccgc ctcttcaccc tgttgataag
    31321 gaacagaaag cccaccgccc gcagaaatag cctgtaaatc ctgaccgaat tcgaggacct
    31381 gacgcaccat agcaccacat acctgttcca gatgggcata atcgacgcca gaaccaatgt
    31441 gcatgtgaat gccgaccagc tgtagatgat gacgttgtat cacgtccagt gcggcgggca
    31501 gatcggtgta ccagataccg tgcttgctgt tttcgccacc ggtattggtt ttttggctat
    31561 gcccgtgacc aaaccccgga ttaacacgca gccatacccg atgccctggc gaaacctgac
    31621 ccagttggtc gagcatatca acagaacccg cattcaccgg aatttgcaat tcactgacgc
    31681 gttcaagcgt cgcctgatcg ataacatctg ccgtaaaaac aatatcatca gggtgcgttt
    31741 gcggattgta acccgccgcc agtgcacgct ctatttcgcc taacgagacg gaatccactt
    31801 tcacgccctg ctcacgcatt aagcgcaaaa tatgaatatt ggaacaggct ttctgtgcga
    31861 agcgcaccac atcaaactgt ttcagcgctg caatctgccg acgaataatt tgcgcatcgt
    31921 agacccacac cgggcagcca aattctgcgg gcaaacgcag cagattttcg gcggtgagat
    31981 cggtatcagt gctgaacagt gaatgtggca taacaaactc cagataagtg cttttttatg
    32041 attacgccac atcataaaaa gaataaaaaa tatcgattta tgtcgagtct atgcaaaaat
    32101 gatatggatt accggattgc gagagagcgc taatggccgc cgttaactta cgtcatattg
    32161 aaatttttca tgcggtaatg accgccggaa gcctgactga ggcggcacac ctgctacaca
    32221 cctcacagcc aactgtcagc cgcgaactgg cgcgctttga gaaggtgatc gggctgaaat
    32281 tgtttgagcg cgtacgtgga cgattgcatc ctaccgtgca aggactgcgt ctgtttgaag
    32341 aagtgcaacg atcctggtac ggactggatc gcattgtcag cgccgcagaa agtctgcgcg
    32401 agtttcgcca gggagaactg tctattgcct gcctgccggt cttttcgcaa tcttttttac
    32461 cgcagctcct gcaacccttt ctggcacgtt atcccgatgt cagcttaaat atcgtgcccc
    32521 aggaatcacc gctacttgaa gagtggctct cggcccagcg tcatgattta ggactcacgg
    32581 agacactcca tacgcctgcg ggaacagaac gtaccgaatt actctcttta gatgaagtgt
    32641 gtgtgttacc tccgggccat ccgctggcgg taaaaaaggt attaacgccg gatgattttc
    32701 acggtgaaaa ctacatcagc ctttcccgta ctgacagcta tcgccagttg ctggatcaat
    32761 tgtttactga gaatcaggtt aaacgacgca tgatcgtaga aacccacagc gccgcgtcag
    32821 tctgcgcaat ggtacgggcg ggggtaggtg tttcggtggt taacccgctc accgcactgg
    32881 attatgcggc aagcggttta gtggtacggc ggttcagtat tgcggttccg ttcaccgtta
    32941 gcctgatccg ccccctgcac cgcccgtcat cagcgctggt tcaggcgttt agtgagcatt
    33001 tacaagcggg attaccgaaa ctggtcactt ctcttgacgc tattttgtcg tcagctacga
    33061 cagcataaaa gcgacagcat cctcggcatg gatcgccgca gtatcaaaca caggcagaac
    33121 actgcgctct tctggcacca gtaaaccaat ttccgtacag ccaaaaatga caccctgtgc
    33181 gccctgttct gcaaggcgag caatcacttg cgcataataa gtgcgtgacg cttcggtaaa
    33241 ttgccccaga cacagttctt caaaaataat ctgattaatt ttcgcccgtt tatccgcttc
    33301 aggaatgaga cagttgatgg aaaattgttc cgtcagccgc ccgcgataaa aatcctgttc
    33361 catggtgtaa cgcgtaccca gcagcgccac acgagtcatt ccagcccgga taattgcacg
    33421 tccagtggca tccgctatgt gtaagaaagg cagagagcaa cgagactcaa tggcatccgc
    33481 cactttatgc atcgtattgg tgcataacac aataccttct gcgcccgccc gctgtaagcc
    33541 aagcgccgcc tcagccagaa tatccccggt tttgtcccac tcaccgcgac gctggcactc
    33601 ttctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa gcccaccaag
    33661 ccgctgttta atgccttcat ttatcagacg atagtaagga atagtggatt cccagctcat
    33721 tcctcccagc aaaccaattg ttttcattcc tctctcctga tgtgtgttac cgcaacaagg
    33781 ctgaataagg aacgagacaa acgcctcaac ggccaggtgc ctaatctcta ttaa
//
LOCUS       DAZKGF010000029        33648 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZKGF010000029 DAZKGF010000000
VERSION     DAZKGF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.029"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            219..668
                     /gene="rpiB"
                     /locus_tag="ACHOQV_001396"
                     /gene_synonym="alsI"
     CDS             219..668
                     /gene="rpiB"
                     /locus_tag="ACHOQV_001396"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFP9629793.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASHVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            737..1066
                     /gene="yjdP"
                     /locus_tag="ACHOQV_001397"
     CDS             737..1066
                     /gene="yjdP"
                     /locus_tag="ACHOQV_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFP9629794.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(1094..1852)
                     /gene="phnP"
                     /locus_tag="ACHOQV_001398"
     CDS             complement(1094..1852)
                     /gene="phnP"
                     /locus_tag="ACHOQV_001398"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFP9629795.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACVRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDQIPVYGPP
                     DEQGCDDLFKHSGLLDFSHTVEPFVMFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVA"
     gene            complement(1854..2288)
                     /gene="phnO"
                     /locus_tag="ACHOQV_001399"
     CDS             complement(1854..2288)
                     /gene="phnO"
                     /locus_tag="ACHOQV_001399"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFP9629796.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYQLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            complement(2275..2832)
                     /gene="phnN"
                     /locus_tag="ACHOQV_001400"
     CDS             complement(2275..2832)
                     /gene="phnN"
                     /locus_tag="ACHOQV_001400"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFP9629797.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            complement(2832..3968)
                     /gene="phnM"
                     /locus_tag="ACHOQV_001401"
     CDS             complement(2832..3968)
                     /gene="phnM"
                     /locus_tag="ACHOQV_001401"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFP9629798.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGKIRAFAESQSRQPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTEAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(3965..4645)
                     /gene="phnL"
                     /locus_tag="ACHOQV_001402"
     CDS             complement(3965..4645)
                     /gene="phnL"
                     /locus_tag="ACHOQV_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFP9629799.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGEEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGVSS"
     gene            complement(4756..5514)
                     /gene="phnK"
                     /locus_tag="ACHOQV_001403"
     CDS             complement(4756..5514)
                     /gene="phnK"
                     /locus_tag="ACHOQV_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFP9629800.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(5511..6356)
                     /gene="phnJ"
                     /locus_tag="ACHOQV_001404"
     CDS             complement(5511..6356)
                     /gene="phnJ"
                     /locus_tag="ACHOQV_001404"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFP9629801.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMNMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWNEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(6349..7413)
                     /gene="phnI"
                     /locus_tag="ACHOQV_001405"
     CDS             complement(6349..7413)
                     /gene="phnI"
                     /locus_tag="ACHOQV_001405"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFP9629802.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVQEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(7413..7997)
                     /gene="phnH"
                     /locus_tag="ACHOQV_001406"
     CDS             complement(7413..7997)
                     /gene="phnH"
                     /locus_tag="ACHOQV_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFP9629803.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(7994..8446)
                     /gene="phnG"
                     /locus_tag="ACHOQV_001407"
     CDS             complement(7994..8446)
                     /gene="phnG"
                     /locus_tag="ACHOQV_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFP9629804.1"
                     /translation="MHADTATRQHWMSVLAHSQPTELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLNADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(8447..9172)
                     /gene="phnF"
                     /locus_tag="ACHOQV_001408"
     CDS             complement(8447..9172)
                     /gene="phnF"
                     /locus_tag="ACHOQV_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFP9629805.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     HFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(9193..9972)
                     /gene="phnE"
                     /locus_tag="ACHOQV_001409"
     CDS             complement(9193..9972)
                     /gene="phnE"
                     /locus_tag="ACHOQV_001409"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP9629806.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(10078..11094)
                     /gene="phnD"
                     /locus_tag="ACHOQV_001410"
     CDS             complement(10078..11094)
                     /gene="phnD"
                     /locus_tag="ACHOQV_001410"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9629807.1"
                     /translation="MNAKIIASLTFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKGNKGLNEQDKLAKTSAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(11119..11907)
                     /gene="phnC"
                     /locus_tag="ACHOQV_001411"
     CDS             complement(11119..11907)
                     /gene="phnC"
                     /locus_tag="ACHOQV_001411"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP9629808.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            complement(12040..12483)
                     /gene="yjdN"
                     /locus_tag="ACHOQV_001412"
     CDS             complement(12040..12483)
                     /gene="yjdN"
                     /locus_tag="ACHOQV_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFP9629809.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTH"
     gene            complement(12643..12978)
                     /gene="yjdM"
                     /locus_tag="ACHOQV_001413"
     CDS             complement(12643..12978)
                     /gene="yjdM"
                     /locus_tag="ACHOQV_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP9629810.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            13380..15609
                     /gene="crfC"
                     /locus_tag="ACHOQV_001414"
                     /pseudo
     CDS             13380..15609
                     /gene="crfC"
                     /locus_tag="ACHOQV_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
     gene            15606..16484
                     /gene="rdcB"
                     /locus_tag="ACHOQV_001415"
     CDS             15606..16484
                     /gene="rdcB"
                     /locus_tag="ACHOQV_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP9629811.1"
                     /translation="MTKTLLDGPGRVLESIHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLARVQLQAWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDAFRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            16748..18250
                     /gene="proP"
                     /locus_tag="ACHOQV_001416"
     CDS             16748..18250
                     /gene="proP"
                     /locus_tag="ACHOQV_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFP9629812.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            18362..18451
                     /gene="pmrR"
                     /locus_tag="ACHOQV_001417"
     CDS             18362..18451
                     /gene="pmrR"
                     /locus_tag="ACHOQV_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFP9629813.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(18427..19527)
                     /gene="pmrB"
                     /locus_tag="ACHOQV_001418"
     CDS             complement(18427..19527)
                     /gene="pmrB"
                     /locus_tag="ACHOQV_001418"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFP9629814.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQNLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19528..20196)
                     /gene="basR"
                     /locus_tag="ACHOQV_001419"
     CDS             complement(19528..20196)
                     /gene="basR"
                     /locus_tag="ACHOQV_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFP9629815.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(20193..21836)
                     /gene="eptA"
                     /locus_tag="ACHOQV_001420"
     CDS             complement(20193..21836)
                     /gene="eptA"
                     /locus_tag="ACHOQV_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFP9629816.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKITPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(21940..23277)
                     /gene="adiC"
                     /locus_tag="ACHOQV_001421"
     CDS             complement(21940..23277)
                     /gene="adiC"
                     /locus_tag="ACHOQV_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFP9629817.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(23414..24175)
                     /gene="adiY"
                     /locus_tag="ACHOQV_001422"
     CDS             complement(23414..24175)
                     /gene="adiY"
                     /locus_tag="ACHOQV_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFP9629818.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(24511..26778)
                     /gene="adiA"
                     /locus_tag="ACHOQV_001423"
     CDS             complement(24511..26778)
                     /gene="adiA"
                     /locus_tag="ACHOQV_001423"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFP9629819.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(26977..27885)
                     /gene="melR"
                     /locus_tag="ACHOQV_001424"
     CDS             complement(26977..27885)
                     /gene="melR"
                     /locus_tag="ACHOQV_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFP9629820.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            28168..29523
                     /gene="melA"
                     /locus_tag="ACHOQV_001425"
     CDS             28168..29523
                     /gene="melA"
                     /locus_tag="ACHOQV_001425"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9629821.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(29520..30170)
                     /gene="yjdF"
                     /locus_tag="ACHOQV_001426"
     CDS             complement(29520..30170)
                     /gene="yjdF"
                     /locus_tag="ACHOQV_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFP9629822.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(30293..31939)
                     /gene="fumB"
                     /locus_tag="ACHOQV_001427"
     CDS             complement(30293..31939)
                     /gene="fumB"
                     /locus_tag="ACHOQV_001427"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9629823.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(32017..33357)
                     /gene="dcuB"
                     /locus_tag="ACHOQV_001428"
     CDS             complement(32017..33357)
                     /gene="dcuB"
                     /locus_tag="ACHOQV_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFP9629824.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         7555 a         8683 c         9334 g         8076 t
ORIGIN      
        1 tcatttaatc actttaaaaa catgatgtta tttgttttcc tggtttatta gatgatagat
       61 gttgctggat agtgagtgtc tttaacgcta ttgcgctgcc agatgtaact ctgtaaaaca
      121 gatcgggaag gcggatttca tcgattttgg gtgtttttaa ttcatttaca tcacaaatgt
      181 tttttgattg tgaagttttg cgcgggcggg gaagatgaat gaaaaagatt gcatttggct
      241 gtgatcatgt cggtttcatt ttaaaacatg aaatagtggc acatttagtt gagcgtggcg
      301 ttgaagtgat tgataaagga acctggtcgt cagagcgtac tgattatccg cattacgcca
      361 gtcacgtcgc actggccgtt gctggcggag aggctgatgg cgggattttg atttgtggca
      421 ctggcgtcgg tatttcgata gcggcgaaca agtttgccgg aattcgcgcg gtcgtttgta
      481 gcgaacctta ttccgcgcaa ctttcgcggc agcataacga caccaacgtg ctggcttttg
      541 gttcacgagt ggttggcctc gaactggcaa aaatgattgt cgatgcgtgg ctgggcgcac
      601 agtacgaagg cggtcgtcat caacaacgcg tggaggcgat tacggcaata gagcagcgga
      661 gaaattgaga ttcgtccact acttgcatgg atgagtaatg actaatgtgg atagagtttc
      721 tttttgaggt tggctaatga aacgctttcc gctatttctt ttattcaccc tgctcacgct
      781 gtccaccgtt ccggcccagg ccgatattat cgacgacacc attggcaata ttcagcaggc
      841 gattaacgac gcttataacc ctgaccgtgg gcgagactat gaagattcgc gcgacgacgg
      901 ctggcagcgt gaagtgagcg acgatcggcg cagacaatat gatgaccgcc gccgccagtt
      961 tgaagatcgc cgcaggcagc tggacgatcg ccagcgccag cttgatcagg agcgtcggca
     1021 actggaagat gaagagcgga gaatggaaga tgagtatggg cgttgatata ggggccggcg
     1081 cgggtatttt ccctcacgcc acctcaatct ccatcccatc aaaccccgcc tcaaaccccg
     1141 atggtagcgc gttttccatc agccacgcat caaactgatg gctgatatgg gtcagaatca
     1201 cccgcggcga gcggataacc tgattcagcg caagcacggt atttaaatca cagtgattac
     1261 gcggtgcatc ctcgcgcggc gggtggctgc aatcaatcac cattacctgc ggctgattat
     1321 tgcgtaaaaa tttcagcgtt ttttccggca ggcctgcggt gtcagatagc cacgccaccc
     1381 ggctgtgcgc cgtttccagt aaatagccga aagtgagttt tgagtggttg agcggtagcg
     1441 gcgtgacctg taatccctgc aaatcaaaca tcacaaatgg ctccaccgtg tggctgaaat
     1501 caagcaggcc cgaatgtttg aatagatcgt cgcagccctg ttcatccggc gggccgtaga
     1561 ccgggatctg atcgcccacg ccccagcgca gcggaaacag cccctgcacg tgatccatat
     1621 ggtagtgcgt cagcaaaaac tgctggaacg atccgggcga ccagcgatcg gcgagatcgt
     1681 gccgcccggc gtcgatcagg gtgatcgcgt cgttaaactt cacgacgccg ctgcatggct
     1741 ggcggcgata ctgcggtgag cgccgcgctc tgacgcaggc cacgcactca cagccccatg
     1801 cgggaacgcc ctgtgcgccg ccagtgccag taagtgtgag ggtcaggctc atgttacagc
     1861 accttggtga agcggaagtg gctctgctcg tagccttcgc gcaggtaaaa tcggtgcgcg
     1921 tcgtggcgct tcacgttggt ggaaagctca gtcatttcgg ccccggcctg gcgggcttct
     1981 tcttctgccc atgccagtaa tttgctgccg acattcagac cgcgcgcctg cggcatcacc
     2041 accagttcct gaatttcgcc gatccagttg acatgatgca gatgaaactg caaatgcagg
     2101 ccgatcatgc cgacaacttc gccatccagc agcgccagtt ggtagcgcat gttcggatcg
     2161 cgcagattgg cgttaaaccc cacgcgaaac gcctggtggt caaactccgc ctgttttagc
     2221 tcacaaatca gcgcgtaaac tgcgtcggtg tcgtactgcg tagccgggcg aagctcacaa
     2281 gcaggcatgg tgtttctcct tctgatgaat cagcgtcagc aacttgtcga ccgactggcg
     2341 caggctgccg tcattattga gcgtaagaca atcctgagga gtatagcggg cagcgcgcgc
     2401 cagccgggcg ttaatttcac tggcattttc acgaccacgg ttttccaggc gctggcgcag
     2461 gatctctggc gaaacctgta aacaaacggg cagcagcgcc gattgatagc gcgcccgcgc
     2521 ctgcggcaga tgggcgcgtg agccgttgac caccacgtca aatccagcgt gcagccagag
     2581 gtcaatctcg acgccgacgc cataatacag accgttagcg tgccagctta aggccagaag
     2641 gttctgcccc gcgcgggtaa aaaactcctg ctcgctcagg gcgatatggt tttcacttcc
     2701 ggcgctggcg gcgcgcgtga tgtagcgatg cgctaccagt agctgagttt gttcccgctg
     2761 gcggagttcc gtcaacagac tgtcttttcc ggagccagag gcccccatta accaaatcag
     2821 ttttcccgtc atcagaacac ccttttaccc tgacgccaga cgtggtcgat atgaatgtga
     2881 ttgcccttgc gatgcgccag caccaggtcg gcgcgtttgc cctcgccaat caccccgcga
     2941 tcctgaagat taagcgcctg cgccggattt ttagtcacca gcttcaccgc ctgcggcagc
     3001 gtaaagcggt tgctctcgtc atcggcgacg cgaaatgccg catcgagcag gctggcgggg
     3061 tagtagtcgg aagagaggat atccagcagg ccaagctgcg ccagttcact ggccgccacg
     3121 ttgccggagt gcgaaccgcc gcgcacgata ttcggtgcgc ccatcagcac gttcatgcca
     3181 tgcttgcgcg aggcttctgc cgcttcgaac gtggtgggaa attcggcgat tacgctgcca
     3241 agttggtgag attcagcaac gtgggcgtgc gtggcgtcat cgtggctggc gagcgcaatg
     3301 tgtcgcgcgc ggcacattgc ggcgatggtt tcgcgatttg gctgcgacca gcgtgcggcg
     3361 agcgccagct gctcttcttc atactgctgc atctgcgctt cggtgagcga gtatttgccc
     3421 tgataatatt cgcgatactt ctcgcggtta gcgaactggc gctggcccgg cgagtgatcc
     3481 atcagcgaca ccagcgtcac cggttcgcgc tgcaccagtt tttcaaacag cggcagcgtg
     3541 gtgtgatgcg gcagttcgca gcgcagatgc agacggtgct cggcgcggtt gacgccgcgt
     3601 ttctgcgtct cttcgatggc gttgatcatc ttctccagat tctccagccg atcgccgccg
     3661 tcgcgcacgt cgccaatcgc caccgcgtcc agcacggtgg tgatgccgct cgccaccatc
     3721 agcgcgtcgt ggctactcat tgccgagtgg gcgggccagt ccacttttgg acgcggggtg
     3781 aagaatttat ccagattatc ggtatgcagc tcaatcagcc ccggcagcag ccagccacct
     3841 tcgccgtcca tcgcttccgg ctggcggctc tggctttcgg caaaggcgcg aattttgcca
     3901 tcctgtactt ccagtgaacc gtgcaccact tcgttttcca gcaccagttt gacgttattg
     3961 ataatcatga agagactccc attgggtgca ggcggtcggc gacgtcattg cgtacggctt
     4021 cgtcatggaa gataccgacg atggccgcgc cacgggcttt ggcttcgcga attaattcca
     4081 ccaccgcggc gctgttttta gcgtccagcg aggcggtggg ttcatccagc agcagaatcg
     4141 gatagtcgac gataaagcca cgggcgatgt tgacgcgctg ctgttcgccg ccggagaagg
     4201 tcgatggtgc caggtgccac aggcgttcag gcacgttcag gcgcgtcagt aatcgggcgg
     4261 ctttagcggc gcaggcttca cgcggtacgc cggtatcgag cagaggctgc atcaccactt
     4321 ccagtgccga aatacgcggg atgacgcgca gaaactggct cacccagccg atggtggttt
     4381 tgcggatctc caccactttg cgggctggcg cggtgaccag gtctacccat tcctcaccgt
     4441 gtttgatctg gatttgccct tcgtcgggca gatagttggc gtacagcgag cgcagcaggg
     4501 ttgattttcc gctgccggag tggccgtgaa gcaccacgca ttcgcctgcg ttgacggtga
     4561 gcgaggcacg ttgtaagacg ggcaggcgca cgccgttttg ctgatgcagg atgaaggttt
     4621 tactgacgtt ttgtacgtta atcatttttc gcctcgtggt tcgggtgtcg gatgcgacgc
     4681 tgacgcgtct tatccgacct acggggagcg catttgtagg ccggataagg cgtttacgcc
     4741 gcatccggca ccggctcaat tctgcaaaac cgatgacacc agcagttgtg tatacggatg
     4801 gtgcgggtcg tcgagcacgc ggtcggttaa cccactctcc accacttgcc cctgcttcat
     4861 caccagcaaa cggtccgcca gcaggcgggc gacgcctaaa tcgtgggtga caatcaccac
     4921 cgccaggttc agctccacca ccaggccgcg cagcaggtcg agcaggcgcg cctgcacaga
     4981 tacatccagc ccgccggttg gctcgtccat aaataccagc ttcggatgcg tcaccaggtt
     5041 gcgggcaatc tgcaaacgct gctgcatacc gccggaaaag gtggtcggca ggtcgtcgat
     5101 ccggttggcg ggaatttcca cctcttccag ccacttctgc gcggtggcac gaatatcgcc
     5161 gtaatgacgc gccccggtcg ccatcagccg ctcgccgata ttgccgcctg ccgacacctg
     5221 gcggcgcagg ccgtcgagtg gatgctgatg caccacgccc cattcggtgc gcagcaggcg
     5281 acggcggtcg gcctcgctca tgccatagag ggaacggttt tcatagcgaa tttccccttg
     5341 ctgcggcgtc aggcgcgcgg agatcgactt cagcagcgtg gtcttcccgg agccggattc
     5401 cccgacgatg cccagcactt cccccggcca taaatcaaaa gagacatcgc taaagccttt
     5461 gcccggcgcg taaaggtggg tcaggttatt gaccgaaagt aacggttgat tcattggctt
     5521 ttcgcctcgc tctgttggcg gcaataatcg gtatcggagc agacaaacat gcggtttccg
     5581 gcgtcatcca gcaccacttc atcaagatag ctgtgggtcg atccgcagat ggcgcatggc
     5641 tcattccact gctgaacggt gaacgggtga tcgtcgaaat cgaggctttc cacgcgggta
     5701 aacggcggca ccgcatagat gcgcttctcg cgccccgcgc cgaacagttg cagggcgggc
     5761 atcatgttca ttttcgggtt atcaaatttc gggatcggcg acgggtccat tacgtagcgc
     5821 ccgtttacct tcaccggata ggcgtaggtg gtggcgatat gaccgaagcg ggcgatatct
     5881 tcatacagct tcacctgcat cacgccgtac tcctccagcg cgtgcatggt gcgggtttcc
     5941 gtttcgcgcg gctcgataaa gcgcagcggt tcggggatcg gcacctggaa aataatgatc
     6001 tgatcttcgg tcagcggcgt ttcggggatg cggtggcgcg tctggataag cgtcgcatcg
     6061 tccgtacgtt cagtggtgtt taccccggtc acgcgcttaa agaagttacg gatcgacacc
     6121 gcgttggtgg tgtcgtccgc gccctggtca atcaccttca gcacatcgct ttcgccaatc
     6181 acgctggcgg taagctgtat gccgccggtt ccccagccgt acggcatcgg catctcgcgc
     6241 ccgccaaacg gcacctgata accggggatc gccaccgctt ttaagatggc gcggcggatc
     6301 atgcgtttgg tctgctcgtc gaggtaggca aagttgtagc cgctcagatt agccatggtt
     6361 ctgctcctgt tgcagacgtt tgagtagctc cagttcggcc tggaaatcga cgtagtgcgg
     6421 gagtttaagg tgcgagacaa agcctgcggc ttcgacgttg tcggcatgcg ccagcacgaa
     6481 ctcttcatcc tgcgccgggc ctgtcgcgtg ctcgccgtac tccggggctt gtagagcgcg
     6541 gtcaaccagc gccatcgcca tcgctttgcg ctcgctcatg ccgaacacca gcccgtagcc
     6601 gcgcgtgaag tgcggcggct catccggcgg gtcgataaaa ccgttgacca tttcacattc
     6661 ggtcatcagc agttcgccga cgttcaccgc aaatcccagc tcttcctgca caatcgacac
     6721 gtcgatatag ccgctgcgga tttcgcctgc gaacggatga ttgcgtccgt aaccacgctg
     6781 ggtggagtag gccagcgcca gcaaatagcc ttcgtcgccg cgcattaatt gttgcaggcg
     6841 ggaggagcgt gagcaggggt aaaccggcgg cgtgcgggtg atgtcgtccg gctgtgcgcc
     6901 gctatcttct tcaaacttcg ccagcccctg acgcgccagc aggctgaaaa cgtgcggcga
     6961 cggctgttgt tcgctgtcgg ctgtggtcag cgtcggcgct tcgccgtttg ccagcagggt
     7021 gaaatcgagc aggcgatggg tgtagtcgta ggttgggcca agcagctggc cgccgggaat
     7081 atctttataa accgctgaaa tacgccgttc gagacgcatc tccgtggtgt cgagcggctc
     7141 gcttactgcg agcttcgcca gcgtggtgcg gtaggcgcgt agcaggaaaa tcgcttcgac
     7201 gttatcgccg ctggcctgtt tcagcgccag cgccgccagt tcgcggtcgg caatgccgcc
     7261 ttcggtcatc acgcgatcta ctgcaagatt aagctgctgt tcaatctggg cgacgctcag
     7321 ttcaggcaaa tcggtattgc cccggcgtcg gctctcttgc agggcgtggg cggcgtcgat
     7381 cgccttctcg ccccctttca cggcaacgta catcagcata cctccacatg agtggttcgc
     7441 gggatagcca gcaggcgctc gccgcaggtc aggatcaggt cgatgccgag cgggaatggg
     7501 tgcgggcgct cggtaagttc gtgcagaatg cactccggca gctgcggggc gatcattcgt
     7561 tcttcggcaa tacccgcgcc agtaaggcgc agcatgcgcc cgccgctcag gctgacgacc
     7621 tgtaaaatca gcgtcgcgcc cgcttctggc gcaacagcgg tgccgctgga aagggcgtta
     7681 agctgttcgc tggaaatcgc ctcatccgtt accgcgaagg tcgccagttc cggctgattg
     7741 accagcggcg cgttggtatg aaaacgcagg ctctggctga cgatatcgtt acttaatggc
     7801 gcagaaagcc acaccggcgt gtcgttatcg gccagcgtca gcagcacgct ggtggtggcg
     7861 atattcagcg gctgccagcc gcgtttgagc tgatgcaggg cgacaatcac gcccggctcg
     7921 ctcatggctt ttaacaggcg acgaaaactg tgctgggcat cctgcacggg aagcataaaa
     7981 gcggtttcca gggtcatgcg ttatctccgc gaaccatcgt aaagaagtcg acccggctgg
     8041 cgtttacttc ggcctggcgt gcggcaatgc gtgccatacg gtcagcgttc agcggggcaa
     8101 taagagtttc tgataagttt tgaaaatggc gggtttgctg catcagcgcg tcaatcagcg
     8161 cgcagcgttc ggcgtgctgt ttgtcgcgcc ccagcaccca gctgtagccg agcgtgccgt
     8221 cagtcaggcg tacggcggcg cgggtgagcg tggcgtcgcc agcaaaaaag cgttcgccgg
     8281 taccacccat ccgcgcctga atctgcacca ggccggtttc agcggcgcgg attacttcat
     8341 agtcggcggt gatgttcagc gcgttcaggc gggctgccag ctcagtcggt tggctgtgcg
     8401 ccagtacgga catccagtgc tggcgggtcg cggtatctgc gtgcattcag tgctccatag
     8461 tgaattcaat catgtcggcg cgcgtcaggc tgacggagta ctccgccggg ctgctttcac
     8521 cgtcacggtg gttaagggtg cgcacgcaca gcagcggcga tagattcggg atttcaagac
     8581 gctggcactc tttggcctgg gcgcggcggg cgctgatccg cgtctgactg cggcgtagcg
     8641 taatccccgt ttgctcgcgc agaaaatcgt gcagcgaacc gctgtcgaaa tgttgcagcg
     8701 tcggccagag ggtgaggtcc gcaaagtagt ggtcgattaa acagagcgcg acgccgttga
     8761 cccggcgcag ggtgcgcagg tggatgacgt tctccccctc ggttatcccc agcgcgtcag
     8821 cgacgtgacc ggaggcgggg cgcagcaccg atagcaattt ttcgctggtg ggatggctgc
     8881 cttggtccag caggttctgg ctaaaacgcg cctgggcgtt gagcgggtaa tcgaacgggc
     8941 gcatcagcac cagcacgccg acgccctgac ggcgctgtac ccaacctttt tccaccagtt
     9001 ggtcgatggc gcggcgcagg gtgtggcggt tcacctcaaa gcgcgctgcc agttgctgct
     9061 cggcgggaag atagtcgccg cagcggtagt gttgacgaag ctcctgctca agttttgcgg
     9121 ctatctcctg atagcgtgtt gggtagctgg tcggatgtgt agacaagtgc atagctatca
     9181 atgcctcgct tatcagataa agtgcttacg caaccgttga gagaggaaat ccagcaggct
     9241 gaccgtgacg atgatgagca ccatcagggc gcaggtttgc tggaactgga aaccgcgaat
     9301 cgcttcccac agggtgacgc cgatcccgcc tgcaccgacc atgccgacga cggtcgccga
     9361 gcggacgttg gattcgaagc gatagaggga gtaggagatc agcagcggca tcacctgtgg
     9421 cagcacgccg tagaggatct cttccagctt gttggcaccg gtggcgcgaa tgccctccac
     9481 cgggccgggt tcgatcgctt ctaccgcttc ggaaagcagc ttggagagca cgccggtggt
     9541 gtggataaac agcgccagca cgccagcgaa cgggccaagg ccgacggcga ccacgaacag
     9601 catggcgaag accatttcgt taatggcgcg gcaggcgtcc atcaggcggc gtacgggctg
     9661 gtaaacccac cacggcacca ggttttcggc gctcatcaga ccgaagggga tggagagaac
     9721 caccgccagc gcggtgcccc agacggcgat ttgcagcgtg acggccattt cggtgaggta
     9781 atcctgccac tggctgaaat cgggcgggaa gaagtcggcg gcgaaggttg ccatgttgcc
     9841 gccgtctttg atcagcgtaa gcggggccat ttccgcgccc tgccacgaga cgaccaacac
     9901 ggcgagaaca atggcccagc tcagaagcga gaaccagctg cgcttgggtg gggcgatggt
     9961 gatggtttgc atgtttggct ccggtctgta ggccggataa ggtgcttgca ccgcatccgg
    10021 catcaacgtc tgcacattgt cggatgcgac gccggggcgt cttatccgac ctacgattta
    10081 ctgcaccgct ttactcaccg aactcatcgc gcttaacgcg ttgttcaggc ggtccaggtc
    10141 atccagttgc gcctgaatgg cgctggtttt tgccagcttg tcctgctcat tcagcccttt
    10201 attgcctttc acgccctgca tctctttaaa cagcgcgagc tggcgaatcg gcaccagttg
    10261 caggtcgctg gaggcgcgga acggggccca gcccaggcgt tccagcaccg ctttctcttc
    10321 cggcgttttg ccgtagttca taaagaagtc gtagatcttg tctttggtgg tttcggacag
    10381 atttttgcgc cagacgatcg gatcgcctgg gatcagcggc gatttccaga tcaccttcag
    10441 ttctttgagc ttctccggtg cggaggtttt cagcttgtcg aggttttcgg tgttgttggt
    10501 ggcaacatcc acctgcttgt tggcaacggc cagcgcgttg gtttcatgcc cggcgttgac
    10561 ggtgcgcttg aagtcgctgg cggagatatt gtttttggcg aagacgtagt agccggggac
    10621 gaggaagcct gaggtggagt taggatcgcc attgccgaag gtgagatctt tacgcttggc
    10681 cagcagatcg ttcaggttgt tgatcggact atctttgttg acgatcaaca cgctccagta
    10741 acccggcgat ccatccgccg cgaccgtctg ggcgaagacc tggccattgg cgcgatccac
    10801 cgcttccatc gctgacagat tgccgtacca ggcgatatcc actttgttga agcgcattcc
    10861 ctggataatg cccgcgtagt ccggggcaaa gaaggcgttg accttcacgc ccagcttctt
    10921 ctccatatcc tgtaagaacg gcgtccattg cggtttcagg ttttgctgtg attccgttga
    10981 aataatgccg aaattcagcg ccttttcctg ctcttcggcg tgcgccgggc ttaacagggt
    11041 gctgaggctg aacatgctgg tgaaggtcag cgaggcaatt atcttagcgt tcattcgttt
    11101 tcctcaatga tggggatgtc aggcagcttt cgcgttttct tcgacgcggt tgatgctgcg
    11161 gtagagatgg tcaaaacgtt cgttatcaaa ctgttggctg ctgccgtcgt agaagacgtg
    11221 cccctggcgc agggcgacga tgcgttcgca gtagcgcagg gcgtaatcca cctgatgcag
    11281 cgtgacgacc acggtaatgc cgtcgttctg gttaatgtcg cgcagggtgt ccatcacgat
    11341 gcgcgccgat tccgggtcca gcgaggcgat gggttcgtcc gccagaatca ccttcgcctg
    11401 ctgcatcagc gcgcgggcaa tcgccacacg ctgttgctgt ccgccggaga gggtggaaac
    11461 gcgctgatgg gcaaagtgca ccatgccaac gcgggtcagc gcctgtaacg cgcgctgttt
    11521 ctgctcgcgg gtgaagtagc taaaacaggt gcgccagaat ggcgtgctgc cgagcgcgcc
    11581 aatcagcacg ttctccagta cgctcaggcg attcaccagg ttgaattgtt ggaagatgta
    11641 gccggtattg gcgcggcttt tgcggatatc gcgggccagg cggccttcgc gctggactgt
    11701 gcggcccagc agctcgatat ggctaccggc agatttatcg ccggtaatca aaccgcttaa
    11761 gtgacgtaaa agggtggatt tgccggaacc cgacggccca agcagagcca ccatttcacc
    11821 gtgatgaatg ttcagatcaa ccgcgtgcag cgcctgatgc tgattgaagg ttttggcgag
    11881 cttctcgaca cggataatcg tttgcatgat gcagcctccc taaaaaagtg gctccatcgt
    11941 ggcggattat tgtgacgatt ttgttaacta aaagtgacta acggatgaag agttaacgag
    12001 atttcgatga cagtgcggga gggcggccct cccggcgggt tagtgcgttg gttgttgttt
    12061 gacgacatta atcatccacg gtacgccaaa tttatcggtg actttgccga agccatgcgc
    12121 ccagaaagtt tcctgccagg ccatttcgat ttttccgtta gctgcaaggt tatcaaacca
    12181 gcgttttcct tcttcgacct gttgcgaatc gagcactagc gtaaagccgg agtagctggc
    12241 ttttcctgac ggtatagcat cgctcatcat gatgtcgctt cctgcaatgc gcacgttggc
    12301 atgagcgatg gcggtatcgg gaaattgcat tccggaaggg cagttctcgg cgctgtcctg
    12361 cgctgatttc ggcatttcgc cgaagctgat tttatagagc agttccgcgc ccaacgtacg
    12421 ttgataatag gcaatcgcgt cggaacagtt accggcaaaa gagaggtagg gacttaacgg
    12481 catgatggtg acctcagtta agagaagccc gttaagtgta gttcgaaatt tatacagatg
    12541 agagggaagc ctgataagac gcggtcagcg tcgcatcagg cagtcgccac cgttgccgga
    12601 tgcggcgtaa acgccttatc cggcctaccg atcacaatac aatcagttct ttttcacaaa
    12661 ctcagacttc agtttcatcg gaccaaaacc gtcgattttg caatcgatgt tatggtcgcc
    12721 ttcaaccagg cggatgtttt tcactttggt gccaattttc agcatcgaag agctaccttt
    12781 cactttcaga tctttaatga tggtaacgct gtcgccgtca gccagcagat tgccgttagc
    12841 atctttaacg atcagctcgt cgctttcctg tgcaggttct gcgtcgttcc attcgtaggc
    12901 acattccggg cagatgtaca tgccgttatc ttcgtaggtg tattcggagt tgcattttgg
    12961 gcagtgtggt aatgacatgt ggatttcctc aaaagtcagc agggcaaaag cgccaaaaaa
    13021 cggcagattg ccgaaaaagg ccgcaattat acacaaaatc cctgagtttg tcgggggtac
    13081 tgctgatttt agacgacgat cgttttcatt tgaaaggaaa cgcaaaaaga aaataatctt
    13141 tttgcctcca tttattattt aagataaggt taatgctcta ctcgttcttt ttttaattca
    13201 catgatgttt ttatctttct gacgttttaa acgtattgga agatgttatt tgttctaata
    13261 aaaaacaaca cgctaaatat aaaattattt cctgcagtaa ttataaaagc cgaaaacaga
    13321 actcaaaaac aatctggcta aataaaagta ataaaatttg ctttaaggaa gaatattcta
    13381 tgtatacaca gaccctgtat gagttaagtc aggaggctga acgcctgtta cagctttctc
    13441 gccaacagtt gcagttactg gaaaaaatac ctctctctgt acccggagac gatacgccac
    13501 aactggcttt accctggagt cagcctaata tcgccgaacg tcacgcgatg ctgaataatg
    13561 agttgcgtaa aatttcccgg ctggaaatgg tgctggcgat tgtcggtacc atgaaagcag
    13621 ggaaatcaac caccattaat gccattgttg gtacggaggt tctgcctaat cgtaatcgcc
    13681 caatgactgc gctgccaacg cttattcgcc atacgcccgg gcaaaaagaa ccggtactgc
    13741 atttttcaca tgtcgcgcca atcgaccatt taattcaaca attacaacag cgcctgcgtg
    13801 attgcgatat taagcatctg accgatgtgc tggaaataga taaagatatg cgtgcgctta
    13861 tgcagcggat tgaaaatggc atcgctttcg aaaaatatta tctgggtgct caacctattt
    13921 ttcattgtct gaaaagtttg aatgatttgg tgcgactggc gaaggcgctg gacgtcgatt
    13981 ttcctttttc tgcttacgcc gccattgagc atattcccgt gattgaagtg gagtttgtcc
    14041 atctggcggg gctggagagt tatcccggtc agttgacgtt actggatacc cccgggccaa
    14101 atgaagccgg gcaaccgcat ctgcaaaaaa tgcttaacca gcagctggca cgcgcctcgg
    14161 cggtactggc ggtgctggat tatacgcaac tgaaatcgat ctccgatgaa gaggtccgtg
    14221 aggcaatttt ggcggtgggg caatcggtgc cgttgtatgt gctggtcaat aagttcgatc
    14281 aacaggatcg taacagtgac gacgccgacc aggtgcgggc actgatttcc gggacgctga
    14341 tgaaaggttg tattacgcca cagcagatat ttccggtgtc gtcgatgtgg ggctacctgg
    14401 cgaatcgggt gcgccatgag ttagccaaca acggtaagtt accaccgcca gagcaacaac
    14461 gctgggtgga agattttgcc catgccgcgc tcggcaggcg ctggcgtcat gccgatctgg
    14521 cggacctcga acatattcgt catgctgccg atcagttgtg ggaagattcg ctgttcgccc
    14581 agccaattca ggcgttgctt catgccgctt acgctaacgc ctcgttgtat gctctgcgat
    14641 ctgccgcgca taaactgttg aattacgcgc agcaggcgcg ggaatacctg gattttcgtg
    14701 cgcacgggtt aaacgtcgct tgtgaacaat tgcggcaaaa tatccatcag atcgaagaaa
    14761 gtttgcagct attgcaactc aatcaggcgc aggtgagcgg cgagattaaa catgaaatcg
    14821 agctggccct gacctccgcc aaccactttc tgcgtcaaca gcaagatgcg ctgaatgcgc
    14881 agttagccgc cttgtttcag gatgattcag ggtcattaag cgagattcgt acctgctgtg
    14941 agacactgtt acagatggcg cagaacacca ttagtcgcga ctttacgctg cgttttgccg
    15001 agctggaatc caccctttgc ccgggtgtta accgatgtta ttcgccccat tgagcaacaa
    15061 atcaaaatgg aattgagcga gtcagggttt cgtcctgggt ttcattttcc tgtttttcac
    15121 ggcgtagttc cccacttcaa cactcgccag ctgttcagtg aagtcatttc gcgccaggac
    15181 gcaatggacg agcagagcac gcgtttaggc gttgtgcgtg agactttttc gcgctggttg
    15241 aatcagcccg actggggacg gggaaatgag aaatccccga tagagacggt tgattacagt
    15301 gcgttgcaac gagcattaag cgcagaagtc gatctttatt gccaacaaat ggctaaagtt
    15361 ctggcagagc aggtcgatga atctgttacg gcaggcatga atactttttt cgctgagttc
    15421 gcttcatgtt tgacggaatt acagacgcgt ttacgcgaaa gtctggctct gcgtcaacaa
    15481 aatgaatcgg tggtcaggct gatgcagcag caattgcagc agactgtgat gactcatggc
    15541 tggatttaca ccgacgctca gctgttacgc gatgatattc aaacactttt cacggcagaa
    15601 cgatattgac caagacgtta ctggacggcc ccggtcgcgt gctggagtcg attcatcccc
    15661 gctttttagt ggatctggcg cagggcgatg atgcccgcca tccacaagcc catcagcagc
    15721 agtttcgtga acgactgatg caggaacttc ttgcgcgtgt gcagcttcag gcatggacga
    15781 acggcgggat gttaaatgcg ccgcttagcc tgcgtctgac attggtggaa aaactggcgt
    15841 cgatgctgga tcccggtcat ctggcgctga cgcagatcgc gcaacatctg gcgctgctgc
    15901 aaaaaatgga tcaccgccag cactctgctt tccctgagct cccccagcaa attgccgcct
    15961 tgtatgagtg gttttcagcc cgttgtcgct ggaaggaaaa ggcgttaacg caacgaggcc
    16021 tactggtgca ggcgggtgaa cagagcgagc aaatttttac ccgctggcgt gctggggcgt
    16081 acaacgcctg gtcgttgcct gggcgctgtt ttatcgttct ggaggagttg cgttgggggg
    16141 catttggcga tgcctttcgt ctgggaagcc cgcaagcggt ggcgttgttg ctgggtgatt
    16201 tgcgcgtgaa agcgacacaa catctggcag agagtatcaa tgcggccccg accacgcgtc
    16261 actattacca tcagtggttt gcctcttcga ccattccgac gggcggggat catgctgatt
    16321 ttttaagttg gctgggaaag tggtccacgg cagataaaca acccgtttgc tggtcagtga
    16381 cccaacgctg gcgaactgtc gcactgggga tgccacgact ctgttcagcg cagcgtctgg
    16441 cgggggcaat ggtcgaggaa atcttctctg taaatttggt gtaaataatc agttacatca
    16501 atgagtccta aacgaaatcc atgtgtgaag ttgatcacaa atttaaacgc tggtagggta
    16561 aaaaggtcat taactgccca attcaggcgt caactggttt gattgtacat tccttaaccg
    16621 gagagtgtaa gcaaacccgc tacgcttgtt acagagattg catcctgcaa ttcccgctcc
    16681 ccttttgcgg ccgtcgcgct gatttttctg gcgtttgcgg aaatgggcca actctgcgag
    16741 gaaagctatg ctgaaaagga aaaaagtaaa accaattacc cttcgtgatg tcaccattat
    16801 tgatgacggt aaactgcgta aagccattac cgcagcatca ctgggtaatg caatggaatg
    16861 gtttgatttt ggtgtttatg gttttgttgc ttacgcatta ggtaaagttt ttttcccggg
    16921 ggctgacccc agcgtgcaga tggttgctgc acttgccact ttctccgttc cctttctgat
    16981 tcgaccgctt ggtggactct tctttggtat gttgggcgat aaatatggtc gccagaagat
    17041 cctcgctatc actattgtga ttatgtcgat cagtacgttc tgtattggct taataccgtc
    17101 ctacgacacg attggtattt gggcaccgat tctgctgttg atctgtaaga tggcacaagg
    17161 tttctcggtc ggcggtgaat ataccggggc gtcgatattt gttgcggaat actcccctga
    17221 ccgtaaacgt ggctttatgg gcagctggct ggacttcggt tctattgccg ggtttgtgct
    17281 gggtgcgggc gtggtggtgt taatttcgac cattgtcggc gaagcgaact tcctcgactg
    17341 gggctggcgt attccgttct ttattgctct gccgttaggg attatcgggc tttacctgcg
    17401 ccatgcgctg gaagagaccc cggcgttcca gcagcatgtt gataaactgg agcagggcga
    17461 ccgcgaaggt ttgcaggatg gcccgaaagt ctcgtttaaa gagattgcca ccaaacactg
    17521 gcgcagcctg ttgacatgta ttggtctggt aattgccacc aacgtgactt actacatgtt
    17581 gctgacctat atgccgagtt atttgtcgca taacctgcat tattccgaag accacggggt
    17641 gctgattatt atcgccatta tgatcggtat gctgtttgtc cagccggtga tgggcttgct
    17701 gagtgaccgt tttggccgtc gtccgtttgt gctacttggt agtgttgcac tgtttgtgtt
    17761 ggcgatcccg gcgtttattc tgattaacag taacgtcatc ggcctgattt ttgccgggtt
    17821 actgatgctg gcggtgatcc ttaactgctt tacgggcgtt atggcttcta ccttgccagc
    17881 gatgtttccg acgcatatcc gttacagcgc gctggcggcg gcatttaata tttcggtgct
    17941 ggttgccggt ctgacgccaa cactggcggc ctggctggtc gaaagctcgc agaatctgat
    18001 gatgcctgcc tattacctga tggtagtggc ggtggttggt ttaatcaccg gcgtaaccat
    18061 gaaagagacg gcaaatcgtc cgttgaaagg tgcgacaccg gcggcgtcag atatacagga
    18121 agcgaaggaa attctcgtcg agcattacga taatatcgag cagaaaatcg atgatattga
    18181 ccacgagatt gccgatttgc aggcgaaacg tactcgcctg gtgcagcaac atccgcgaat
    18241 tgatgaataa gctgaaacgg atggcctgat gtgacgctgt cttatcaggc caattgaact
    18301 cttaaggttc acttaatctc tgacgcgcat actctcctcc aggttaacgg aggagagtgc
    18361 aatgaaaaac cgtgtttatg aaagtttaac taccgtgttc agcgtgctgg tggtcagcag
    18421 ctttctttat atctggtttg ccacgttctg atctttcttc agccgtaccc aggcccgcgt
    18481 gccggaagtc tcttgccggt tttgcaggaa aaactgcccg tgatgcaact gtgtaatgcg
    18541 gctgacaata cttaacccca gaccaatccc gccataacgg ctgtccatac gtacaaacgc
    18601 tttactcaac tccccgcatt tactctcatc aatacccggt ccttcatctt caacggccat
    18661 gaccgctccg ccatcttctt gcagcttaat cataatgttg ctgccttgcg ggctgtaacg
    18721 atgggcgttt tctaccaggt ttcgcaataa catccgcagc agggttgcat caccctgaac
    18781 ggtgatgtcg gcggcgctct ctggcaatag caggttttgc tgtcgctggt cgagcatggt
    18841 actgagttcg tcatacgagg ggagaatgac atcttccagc agttttacat gttgataatt
    18901 accggaagaa aatgactgtc cggcacgcgc cagttgcagc agctgggaga cactctccat
    18961 catctgatca agtcgagcca ctaacggtgc tacatcaatg tgatgcgttt tcgccagcag
    19021 ttccagatgc aaacgcaccc ccgccagtgg cgttcgcagt tcgtgcgcga cgtcagcggt
    19081 aaacaacctt tcgttatcca gcgtgctggt caggcgactg accagatcgt tcaacgccga
    19141 aaccaccgct tcgatttcga gggtggcgct gtgaatggca ataggcgtta agttgtcggc
    19201 ggtgcgcgct tccagctctt tttgcagctc cgccagcggc cgggtgatgc ggcgtaccgc
    19261 ctgatagcag ataaatagcg tcaggctgac cataaagacg ccggggacga tcaggctggc
    19321 gaccgcctcg cggatctcac gcatgatgtg gcgatcgttg ttgcgattgt cgcgtagcgc
    19381 ctgctcaaac agctgaatct gctcggtact ttcatgccat agccagaaga cgctgatcag
    19441 ctcaaacacc aacaaaatgg ccccaatggt caatatcagc cgttggcgca gcgatattgg
    19501 tcggcgcaga aaacgcatca gattcaatta gttttcctca tttgcgacca gcatatagcc
    19561 aaagccgcgc acggtgcgga tacgggcttt gcccactttg tcgcgcagat tgtggatatg
    19621 cacttccagg gtgttggtcg agggttcatt gtcccagtta tagatgtcgt tgtagagaat
    19681 ttcccgatgc accggactgc ctgctttgag cattaaccgt gacagcagag catattcttt
    19741 gggcgtcaga atcaactctt caccgcccat ccatacctga cggcgaccca tattcagcgt
    19801 cagattgcca acaatcagct cactttcgcc ctgattatta tggcgtcgta gcagggcgcg
    19861 gatacgggcg tgtaactctt ccagcgcaaa aggcttcacc agatagtcat cggcaccgac
    19921 atccagcccg gcgattttgt cggtcagcgt atcgcgagcg gtgaggatca gtaccggcag
    19981 ggtgtatttt ttctgccgga tacgggcgag aaaatgcagt ccatcttcgt cgggtaaccc
    20041 taaatccagt accaccaggc tgtaatgacc ggcctcaagg ctttgttccg ccatccgcgc
    20101 ggttgtcacg ccatcgcacg cgtagccttc ggtttgcgcc gccagaatta atccctgcaa
    20161 taacagcgta tcgtcttcaa caatcagaat tttcattcac tcactctcct gcaagtttgc
    20221 agaatatcat ccgcagcctg gtaatacttc gtctcaacgc cagttaatcc caatagcgtg
    20281 gagaataaat tatcttgtga atagtgttgc gtttgcgcct gtttttgcag gcagttctgg
    20341 tcaacctgat accgtttttg ataatcctcc gacagccaca gcagcatcgg cacctgtttt
    20401 tggctatccg gggcgatggc ataaggcaga ccgtgcagat agatgccatt ttcacctaac
    20461 gactcaccgt ggtcagaaag ataaaccagg ctggtggtaa atttatcctg atgttctttc
    20521 agcagattaa tcgctttatc aacaatatag tcgacgtaaa ccagcgtgtt gtcgtaagtg
    20581 ttcaccagtt gctctttggt acaggtctgg atctcgttag tgtcacaggt tggggtaaat
    20641 ttcctgaact gcggcggata gcggttgtaa taggtcggac cgtggctgcc gatggtgtgt
    20701 aagacaatca cgccatcacc ctgcaggtta ttgatgtact cttccagccc gtggaacagc
    20761 acttcgtcat agcattcgcc gttgatgcac tgacctggca gattcagcgc ggtgacgttc
    20821 tggtgaggta cgcgatcgca aacgccttta cagccgccat cgttgtcatt ccacagcacg
    20881 ttgatgcccg ctcgctgaat gatatccagc acgccttcct ggtgctgtgc cagctcttct
    20941 ttgtagtgct cacgcggcat atccgagaac atgcatggta ctgaaactgc cgttgccgtg
    21001 ccgcaagagg cggtattagg gaaatagacc acgttatctt ttgccagacg aggattggtt
    21061 tcgcgtggat aaccattcag cgagaaattc tctgcgcgcg acgtttctcc gacgatcaaa
    21121 atggtcaggt ttttacgttt cccattttgc attaatgggt tgcggtgagc atcttcacca
    21181 atgcgtatca gtggcaggtt ggctgaccgc tggtgagagt accacgacca gctagcaaca
    21241 atgctgttgg acgggcttaa ggatttcacc agctctttgt tgttgcgaaa cagcgaggca
    21301 tagtctttgt agaacagtgc ggcaaccagc agaatcagca gtacagaaat caggatgttg
    21361 gccccgcgga aaagaacgct gcgcaggcgt gaggtggtgg gggtgatttt gatccagcag
    21421 gcaataatcg cggctaacac gccacttaac cccagcgtta ataacattcg tggcgtcatc
    21481 agggcataac tttccgccgg cgtagtatca atgatattgg tgatcattga acggtcgatg
    21541 acgatgccat aggtcatgat gaaatattgt gcagccgcac cgaccagaat aaacaggcag
    21601 gcgagtggac gattaagcca taagaaagag cctagtgtca ggacaatatt aatcacgctg
    21661 aatgcgacga ccggcatcga caagaaaacc agcacgttat gcagtgaatc cagcggtaac
    21721 acctgtagca cctgtttaaa aaaggcgata ttcaggcaga ctgagatata aaaagtagca
    21781 agtaataacc aggagaataa atttaaagag ggtcttttta gtaggcgctt caacatcacg
    21841 gtgtttccat cgaacaaagt gcgcatatgc tcgcaaagca aaattaagcc aaccttaagt
    21901 tcttaaggtt ggcttttatg tttgcttgat taaggggaat taatctttgc ttattggtgc
    21961 atctaaggga tacgggtttt tatgcagccg gttgtaattc agcgcataca tggcggtgat
    22021 taccatcaga gtgacaaatg accacataac ctctttcgcg ccggacccta ccacggccca
    22081 gatgcagtag aggaaggcaa tggtagtcac tgccaggtat gccgggcgtg ctttaccaaa
    22141 gtgaccgtgg ccgagcagta gtaacgccgc acaggtgtaa agatatggca ccagtgtaaa
    22201 gatgaccgac acggaagaaa ccagaccgaa ttctttggtc gcgtttggcg aaatgctact
    22261 gagctggaag atggtcatca aaataccgac gataatcagc cccgccactg gcgtgcccgc
    22321 tttattaaca cgggcaaaaa tcggtgggaa cagtccgtca tcggcagcgg ctttcgccgt
    22381 ttgacccgcc agcaacgtcc agccgcccag tgaacctaag caacccgcag ctgcgcagaa
    22441 ggaaacaatg gccccggcgg tgtcacccaa cgccatccgt gcggcatcac cgaatggcga
    22501 agcagaaacg cgcagtgcgg cattagggat catccccata atcgcggtgg tagaaagtac
    22561 atagcaaacg gcagcaatca ataccccgcc aatggtggcg atagggacgt tgcgtttcgg
    22621 gtttttcacc acacctgcgg caacggaggc actttccaca ccgatgaacg accacagcgt
    22681 aacgttaagg gtactttgaa tcgcaccgaa ggtgcccaga ccgctgacgt tccatgccgc
    22741 catataggtt tcaccacgga accagaacca gccaaatacg gcaatcccga cgatggggat
    22801 cagcgccagc acggtagcaa cggcctgtac gcgggtgatc attttcggac cgacaatgtt
    22861 cagcaggacg aagatccaca gcaccacgac gcaggtgatg gttaatacca atgggtcttt
    22921 cagaatcggg aagaagtaac ttaaatatcc tacgccaatg accaccatgg cgatattgcc
    22981 gatccagcag gccagccagt agaggacgtt ggtttgataa ccgagaaacg ggccaaagca
    23041 gcggcgggcg taagcgtaag aaccaccagg acttgggtcg aggaacgaca ttttggcgta
    23101 taccatcgag agccccagcg caccgataat cgtcaccaac catccataaa tggcaatccc
    23161 gccagtggag gccaggtttg caggtaacag aaaaacacct gaccccataa tattccccga
    23221 caccatcagg gtgacgggga ttaagcccac tttgtgagca tcagcatccg aagacataat
    23281 taaactcctg cgaaggcgag cttcgtgaca ataaattacg tcgtatcata cgcctgcatt
    23341 cgatagttga attattatcg gccgggttat cgctgatgcc gttattaata tagctcttcg
    23401 ttaaggttaa aaatcaggcg acagtgcgtt ctctgtgctg actaacataa tgcagcggcg
    23461 tcataccgta gaagtcttta aagacagaaa taaagtacga cgtactgttg tagccgcagg
    23521 actgtgatac ctgagagata tttttaccgt ccatcattaa ttcatttacg gcatagcgca
    23581 tgcggcaggt ggtgattatt tggctataac tggtgttttc gcttttcagc tttttcttta
    23641 acagacttgg gctaagacat aaacaactgg ctaccatact aagattccag tctttgtgga
    23701 tatcgctttc aataatttga taaacgctgt cacttacaca attacgtaac atatacatca
    23761 ttagtgaaag gaatttttta ctgtcgagaa aacgggatag cacagtgaat aataatgcac
    23821 gtgttcgttc cttttctgac tcagtttcag cgggcatcat gctatgttgc gcggcaacgc
    23881 gaaagacgtc tggcgtcagg catggaaggg tgaggatggg cgtagcgcta cgttgccata
    23941 ctggtatttg cgagagatct ttattcagaa accggaggta atctttgatg atgtcgtgac
    24001 taatatgagc aaccaaagtg ttattaagag aggagacgtc gataatatta ttttcacaat
    24061 ttaataacgc catatggtta gctttaaggc taataggttc tttcccattc acccttatcc
    24121 agacatcttt ttcagtcaat aaaacaatac aaggttggtc gctgcaaatc ctcatacatg
    24181 tactcctgag tgcgaataaa agaccagttc tcgctaaagc aaagcgatac cgggaaatgg
    24241 ttatataatt gtatgatgaa tataaacatg cataaaaata acgagtttta ctaatattac
    24301 aaatttttat agtaatttct catgtacata tttttttaca ggctaaaaaa aggataaata
    24361 acaggcaaaa aaaagcgcgg ggtgggaccg cgcaaaaagc atgacatttt ttgtttgcag
    24421 caatttttat gaaaacgttg ccatacttaa aaggcctcaa gccaggcggc gtaatgttat
    24481 ttaacaatta cgccttcagc ggaatagtgg ttacgctttc acacacataa cgtggtaaat
    24541 accgtcaata atttcagtcc cttcagtttc gtgttcaaat ccagggaaat gatggtccca
    24601 ggattgcagc gagcgtaaat aacttacttg cggactgttt ttatcgccga agttttcacc
    24661 agacagcagc atcgggattc ctggcggata cgggataact gagtttgccg cgatgcgtcc
    24721 tggtaggttt tcaatggata ccagttcgac attgttgtcg acaatcgcgt tgtacgcttc
    24781 tcgcggggtg atttccgcca ccggcaggcc ggaataggct tcgttcaacc gtgcgccagg
    24841 gttgttttct ttcagccagg caaacatggt gtcacccaga tcgtgaatcc ccatgttcgc
    24901 gtaagtgtca ggatattgtt caacaagttc cggcatcacc tgcgccagcg gggtgttggc
    24961 gtcatagtgg cgtttgaagg agcaaagggt gttaaccaga gtcccccatt tcccacgggt
    25021 cacgcccata gagaacagga acataatctg gaagtcagtg gtacgggtag gcacaatgcc
    25081 gtggcgacca agccaggctg tgaccagcgc cgccggaaca ccggtttctt ccagttcacc
    25141 atcttcaccc attcccggag caaggatgct gactttaatc gggtcgagca tactccagtt
    25201 atccggaata tctttgaagc cgtgccagct ttcgcccgga tgcatgaccc agcagtcctg
    25261 aacggtggtc agcagtttgg ttggtgcgtc agcaaagtca taggttttgc cggtttgtgg
    25321 gtcggtgacg acttctttgt tccacggttt gaagaaccag ctaccgtcag cggtgaactc
    25381 tttatacagc cgcgccatcg cctgacggaa atcaaccgct tcgtcgatca cttcctgcgt
    25441 cagtgacagg ccgctgttgc cgtccatcat cgacaccgcc acgtcgttgg atgcgcagat
    25501 ggcatacagc ggggaggtgg tggcatgcat catgtaggcc tggttgaagc gggagaagtt
    25561 aatcgcccca cgaccttcac gtacatgaat ataagaggcc tgtgacagcg cattcagcag
    25621 tttgtgggtg gagtgggtgg cgaaaacggt agggccgttg tgatcgccag gttcgccgcg
    25681 catggcatag tgatcggcat agattgggtt gaaacgtgca tagccgtacc aggcttcgtc
    25741 aaagtgcaga cgatcggagg ttttttccag cagatcctgc gcttctttag cgttataaca
    25801 cacgccgtca taggtacagt tggtcaccac gcagtaagac ggtttttgcc cggctttgtc
    25861 tttggtcagc gggctttcac tgattttctt ctgcaaggtt tcaggttgca tttcctgcgg
    25921 atagattggc ccgataatgc cgtagcggtt gcggcttggc accatgtaga ccggtttcgc
    25981 gcctgtcagc atcaagcctt gttcgatgga tttatggcag ttacggtcaa cgaccacgac
    26041 atcgttatcg gtcatgcaag cctgcatgat ggtgcggtta gagccggaag taccgacgac
    26101 taccgaccag gagcgatcgg caccaaatac gcgtgcagca tatttttcgc tttcgccaaa
    26161 tgcgccagta tggtcaagca aagaaccgag ggaagttcgt tcgatgccca tgtcggtgcg
    26221 gaacagattt tcaccatagt agtcatggta gaaacgtccg gcgggtgttt tggtaaaacc
    26281 aacgccgccc tggtggcctg gcgctgccca ggaatattca tggatgtcgc tatatttcat
    26341 cagcgcgctg aacagtggcg gcaacagttg ctggcggtag cgggtcatcg cggcaacggc
    26401 gcgtccggcg ataaagtcgg cggtatcttc cagaatccag gcgaattcat cgacaagctc
    26461 cagcagatcc cggtccattg cggcgagggc tttttcccga tcgcccaaca ggaagaccgg
    26521 cacgttttgt tgacgctcat gaagcttacc gatcaattgt ctgacgtttt gatgttcgtc
    26581 cggatgttcc atttgatagc tgaacatcag gcagtcaatg gcttcgtttg aagagagaat
    26641 ggcaaaacca tcatcaaagg aggtggattt aatcacggta acattttgct ggcttaaagc
    26701 atctgccaga cgctcaacgg cgttaccgac ccaggtgtct tgatggagaa actcgctttc
    26761 aacaattaat actttcatca ttgcttaccc ggttatgaag gaatcttcgt tgcgggcaag
    26821 tatcttccgg ccttttcggg ctgtaaagcg cgtgaaaaac aaacattttt gaaatatttg
    26881 tttttataaa caatatgtta tgtgattatt ttaaattatt ttcactgctt attatcgacg
    26941 gctaaactat ttttttggct gatactgata tcgtctttag ccgggaaacg tctggcggcg
    27001 ctgttggcta agtttgcggt attgttgcgg tgacatgccg acatatttgc cgaacgtgct
    27061 gtaaaaacgg ctactcgaac gaaagcctgc cgtcagggca atatcgagaa tacttttatc
    27121 ggtatcgctc agtaacgcgc gaacgtggtt gatgcgcatc gcggtaatgt actgtttcat
    27181 cgtcaattgc atgacccgct ggaatatccc cattgcatag ttggcgttaa gtttgacgtg
    27241 ctcagccaca tcgttgatgg tcagtgcctg atcatagttt tcggcaataa agcccagcat
    27301 ctggctgaca taaaattgcg catggcgcga gacgctgttt ttgtgtgtgc gcgaggtttt
    27361 attgaccaga atcggttccc agccagagag gctaaatcgc ttgagcatca ggccaatttc
    27421 atcaatggcg agctggcgaa tttgctcgtt cggactgttc aattcctgct gccagcggcg
    27481 cacttcaaac gggctaagtt gctgtgtcgc cagtgatttg atcaccatgc cgtgagtaac
    27541 gtggttaatc aggtctttat ccagcggcca ggagagaaac agatgcatcg gcagattaaa
    27601 aatcgccatg ctctgacagg ttccggtatc tgttagttgg tgcggtgtac aggcccagaa
    27661 cagcgtgata tgaccctgat tgatattcac tttttcattg ttgatcaggt attccacatc
    27721 gccatcgaaa ggcacattca cttcgacctg accatgccag tggctggtgg gcatgatatg
    27781 cggtgcgcga aactcaatct ccatccgctg gtattccgaa tatagcgaca gcgggctgcg
    27841 ggtctgtttt tcgtcgctgc tgcacataaa cgtatctgta ttcatggatg gctctctttc
    27901 ctggaatatc agaattatgg caggagtgag ggaggatgac tgcgagtggg agcacggttt
    27961 tcaccctctt cccacagcgg cgaggggagc gtccgagcat catgaggccg aaaactctgc
    28021 ttttcaggta acttattccc ataaactcag atttactgct gcttcacgca ggatctgagt
    28081 ttatgggaat gctcaacctg gaagacggag gttttctgca gattcgcctg ccatgatgaa
    28141 gttattcaag caagccagga gatctgcatg atgtctgcac ccaaaattac atttatcggc
    28201 gctggttcga cgattttcgt taaaaatatt cttggtgatg tgttccatcg cgaggcgctg
    28261 aaaacggcgc atattgccct gatggacatt gatcccaccc gcctggaaga gtcgcatatt
    28321 gtggtgcgca agctgatgga ttcagcaggg gccagcggca aaatcacctg ccacacccaa
    28381 cagaaagaag ccttacagga tgccgatttt gtcgtggtgg catttcagat tggcggttat
    28441 gaaccttgca cggtgactga tttcgaggtc tgtaagcggc atggtctgga acaaaccatt
    28501 gccgatacgt tggggccggg cggtattatg cgcgcgctac gtaccattcc gcatctgtgg
    28561 caaatttgcg aggacatgac ggaagtctgc cccgatgcca ccatgctcaa ctacgttaac
    28621 ccaatggcga tgaatacctg ggcgatgtat gcccgctatc cgcatatcaa acaggtaggg
    28681 ctgtgccatt cggtgcaggg aacggcggaa gagctggcgc gtgacctcaa tatcgacccg
    28741 gctacgctgc gttaccgttg cgcaggtatc aaccatatgg cgttttacct ggaactggag
    28801 cgcaaaaccg ccgacggcag ttacgtgaat ctctacccgg aactgctggc ggcttatgaa
    28861 gcagggcaag caccgaagcc gaatattcat ggcaatactc gctgccagaa tattgtgcgc
    28921 tacgaaatgt tcaaaaagct gggctacttc gtcacggaat cgtcagaaca ttttgctgag
    28981 tacacaccgt ggtttattaa gccaggtcgt gaggatttga ttgagcgtta taaagtaccg
    29041 ctggatgagt acccgaaacg ctgcgtcgag cagctggcga actggcataa agagctggag
    29101 gagtataaaa acgcctcccg gattgatatt aaaccgtcac gggaatatgc cagcacaatc
    29161 atgaacgcta tctggactgg cgagccgagt gtgatttacg gcaacgtccg taacgatgga
    29221 ttgattgata acctgccaca aggatgttgc gtggaagtag cctgtctggt tgatgctaat
    29281 ggcattcagc cgaccaaagt cggtacgtta ccttcgcatc tggccgccct gatgcaaacc
    29341 aacatcaacg tacagacgct gctgaccgaa gccattctta cggaaaatcg cgaccgtgtt
    29401 taccacgctg cgatgatgga cccgcatact gccgccgtgc tgggcattga tgaaatatat
    29461 gctcttgttg acgacctgat tgccgcccac ggcgactggc tgccaggctg gttgcaccgt
    29521 taaggggtaa tcgcttccgg cgctctggtg attaagccaa agcgccgtag ctggcggcaa
    29581 tgaaagcgag cgaggaatat caccgtcgtt aatgcgccaa gcagcgcgca gaacatatcg
    29641 gattgcgtgt cccactggtc gccctgggtg ccgagaaaat catccgctcc ctgacccatt
    29701 gccagcgccg cccaccactc gattaattca tacatcgcgc ttatcgccag cgccacgcag
    29761 cagaccagaa acgccaccat cttacgtccg cgaacgtaca tcccgcgtac aaggatctct
    29821 cgtgccacca gtgcaggcac cagcccctgg aaaaaatgcc ccagtttgtc atacggatta
    29881 cggctcaacc ccagccattc ctgcacctca aaaccaacgg ggactttcgc gtaggtgtat
    29941 tgcccgccga ccatcaggat gatggcgtga aagaaaatga gcgtataaag cagcggagtt
    30001 aacggataac gtttagcggt ggcaagcagt agcggcacga caataatcac cggtgtcact
    30061 tccatcagcc aggtgagttt gtcatgggcc gaaatgccgg tataaatcag gattaacgtt
    30121 agcgccagtg cgccggtgtt aagaattaac ggcttgagtg tgcgggtcat ggtgagttcg
    30181 caagtcaggg aaaatactga ctattgacca gtaagcgtga aattacaatc gcaaaagagg
    30241 tggcccaggg ggatcacctg gcagcatgct gccaggcgct gggccgaaga ggttacttag
    30301 tgcagttcgc gcactgtttg ttgacgattt gctggaagaa gtcgttgcct ttgtcatcga
    30361 ccaggataaa cgccgggaaa tcttctactt cgattttcca gatagcttcc atacccagct
    30421 ccggataagc gacgcattcc agatgcttga tgctctgctg cgccagtacc gccgccggac
    30481 cgccgatact accgaggtag aagccgccgt gtttatgaca cgcgtcggta acctgctgac
    30541 tgcggttacc tttcgccagc atgatcatgc tgccgccgtg ggattgcagc agatccacgt
    30601 aggagtccat acgacctgcg gtggttgggc caagtgaacc tgatggataa ccggcagggg
    30661 ttttcgccgg acccgcgtag tagatcgggt gatctttgat gtactgcggc agttctttac
    30721 cggcgtcaat cagctctttc agcttggcgt gtgcaatatc tcgacccaca ataatggtgc
    30781 cggtgagcga caaacgggtg gataccgggt attgcgaaag ctgggcgagg atctctttca
    30841 tcgggcggtt aaggtcaact ttcaccgctt cgccttcacc ggcctggcgc agttcttgtg
    30901 gaatgtactg gcctgggttg tgttccagtt tttcgatcca gataccttcg cggttgattt
    30961 tcgctttaat gttacggtct gcggagcagg agacgcccat gcctaccggg caggatgcgc
    31021 cgtgacgtgg cagacggata acgcgaatgt cgtgcgcgaa gtatttaccg ccaaactgcg
    31081 cgccaagacc gagtttctgg gcctcttcca gcagttcctg ttccagctgg acatcgcgga
    31141 acgcctgacc atgttcgttc ccttccgtcg gcagttcatc gtaatagtga gcgcttgcta
    31201 acttgacggt tttcaggttg gtttccgcag acgtaccgcc aatcacaaac gcgatatggt
    31261 acggcgggca agctgccgta ccgagggtac gcattttctc gacgaggaag tttttcagtt
    31321 tgccgggcgt cagcagggct ttggtttcct ggtagagata cgttttgttg gcagagccgc
    31381 cgcctttagc gacacagagg aatttgtact catcgccatc taccgcgtac aggtcgattt
    31441 gcgcaggcag gttcgtgccg gtgttgacct ctttatacat gtccagcggc gcgttctgtg
    31501 aatagcgcag gttatcttcg atataggtgt tatagacgcc tttcgacagc gcttcttcat
    31561 caccgccacc ggtccacacg cgctgacctt ttttaccgac gatgatcgcg gtgccggtat
    31621 cctggcaggt cggcagcacg cctttggcgg cgatttcgga gtttcttaag aattgcagcg
    31681 ccacgtactt gtcgttttcg ctggcttctg gatcgtgaag aatagccgca acctgtttct
    31741 ggtgtgctgg gcggagcatg aaagaagcgt cgtgaaaggc ttgctgcgcc agcagggtca
    31801 gggcttctgg ttccactttc aggatggttt cgccgtcgaa gtcggcaacg ctaacgtaat
    31861 cggaagtgag tagatagtat tcggtattgt ctttccccat cgggaaaggt gcctggtaga
    31921 taaagggttt gtttgacata gcttccagcc tgtaactctg tatttgttat tcgaaaatgg
    31981 cgtgccgcgt agtcacgggc ggcacgcaaa gtgcatttat aagaacccgt acatcgcggc
    32041 gaagatccag ccgaagacgc acgatacgct cacaccaatc aaccccggca gaataaagct
    32101 gtggttgatg acgaagcgac cgatgtgggt ggtgccggaa cggtcaaact gaatcgctgc
    32161 cagatcgctc ggataagtcg gcaggatgta ataaccgtag caagccggtg ctgaagccac
    32221 gatgtatgcc ggatcaacgc cgatcgccag cgcgaccgga acaatcgccg ccagcgccgc
    32281 agcctgagag tttacaaact tggaaaccag cagcagaaca atggcatagg cccacggata
    32341 ctctttcacc atttcaccca gtacgccctg aatttcagac atatgcgcac cgaacatggt
    32401 ttctgccatc catgcgatac cgtacaccgc cacgatggcg atcataccgg aacggaagac
    32461 ttcgtttttt gagatagacg cgggattggt tttggtcagg ataataatca gcgccccggt
    32521 cagcagcata aacatctgaa taaccagtac catcgacagc ggtttgccgc cgaaggatgg
    32581 acgcaggtcc gaatcagcac caagaagggc gactacagcg attgccccga ggaagatcca
    32641 cattgccagc cagttgcttt tcggcagttt tttatccagc agcgtcgcgg tatcaccgta
    32701 aacatactca cggttttccg gtacggagat gaatttctgg aactcttcgt ctttatccag
    32761 atctttaccg cggaaccagc tgaagatacc gatcgccagg ataccgatta acgtcgatgg
    32821 aatggtgatt gccagcaggt cgaggaactc aagatggcga ccatcaaagg tgacattacc
    32881 cagcatcgca accagagaca cgactgcaac cgacaccgga ctggcgataa tccccatctg
    32941 tgcaccgata gaacttgccg ccattggacg ttccggacgg atgttgttct taatagcaac
    33001 gtcgtagatg atcggcagaa tggtgtaaac cacatgaccc gtaccgcaaa gaatggtcag
    33061 cgtacaggtc acaaacggcg cgacaattga gacatatttc gggttgcggc gcagcagctt
    33121 ctcggcaatt tgcagcatga catcaagacc gcccgaagct tgcaaggtcg ccgatgccgc
    33181 caccaccgca atgataacca gcatgacatc aaccggtggt ttacctggct gaaggtggaa
    33241 gacgaagacc agaatgacca gaccgatacc gcctaataaa cccagcgcga taccaccctt
    33301 tctggcacca taaaacagac atatcagtat tatgataagt tggatagtaa ataacatgtg
    33361 tgaaccctcg cgataatcct atttaaattt ttgctgaata gatcacagtc acgttctgtt
    33421 ttgtatgaac tgttttcaga gcctgactaa atatccgttt ggtcgttacc ggctttagca
    33481 aatacctcac agtgaatatt ggctgattaa agcagcggca aatttatgca ctgtgataag
    33541 cggctttttc agagagaaaa actctctggc gatgggttta tagtttgtac tgcgcagatg
    33601 ttaatgtttt gtttgctgtt atcttattga tatttatcgg tctatttt
//
LOCUS       DAZKGF010000030        33405 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZKGF010000030 DAZKGF010000000
VERSION     DAZKGF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.030"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(138..278)
                     /gene="yjjY"
                     /locus_tag="ACHOQV_001429"
     CDS             complement(138..278)
                     /gene="yjjY"
                     /locus_tag="ACHOQV_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFP9629825.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            374..1090
                     /gene="arcA"
                     /locus_tag="ACHOQV_001430"
     CDS             374..1090
                     /gene="arcA"
                     /locus_tag="ACHOQV_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFP9629826.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1149..2501)
                     /gene="creD"
                     /locus_tag="ACHOQV_001431"
     CDS             complement(1149..2501)
                     /gene="creD"
                     /locus_tag="ACHOQV_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFP9629827.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAHWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFIAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGAIMNGIYLQAVLKGWCNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2559..3983)
                     /gene="creC"
                     /locus_tag="ACHOQV_001432"
     CDS             complement(2559..3983)
                     /gene="creC"
                     /locus_tag="ACHOQV_001432"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFP9629828.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDTTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPTDPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVEQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3983..4672)
                     /gene="creB"
                     /locus_tag="ACHOQV_001433"
     CDS             complement(3983..4672)
                     /gene="creB"
                     /locus_tag="ACHOQV_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFP9629829.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLTLHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4685..5158)
                     /gene="creA"
                     /locus_tag="ACHOQV_001434"
     CDS             complement(4685..5158)
                     /gene="creA"
                     /locus_tag="ACHOQV_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFP9629830.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5369..6238
                     /gene="robA"
                     /locus_tag="ACHOQV_001435"
     CDS             5369..6238
                     /gene="robA"
                     /locus_tag="ACHOQV_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFP9629831.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6235..6882)
                     /gene="gpmB"
                     /locus_tag="ACHOQV_001436"
     CDS             complement(6235..6882)
                     /gene="gpmB"
                     /locus_tag="ACHOQV_001436"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFP9629832.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6934..7449
                     /gene="yjjX"
                     /locus_tag="ACHOQV_001437"
     CDS             6934..7449
                     /gene="yjjX"
                     /locus_tag="ACHOQV_001437"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFP9629833.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            complement(7443..7769)
                     /gene="trpR"
                     /locus_tag="ACHOQV_001438"
     CDS             complement(7443..7769)
                     /gene="trpR"
                     /locus_tag="ACHOQV_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFP9629834.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKDH"
     gene            complement(7859..9796)
                     /gene="sltY"
                     /locus_tag="ACHOQV_001439"
     CDS             complement(7859..9796)
                     /gene="sltY"
                     /locus_tag="ACHOQV_001439"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFP9629835.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWELSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10132..11799
                     /gene="ettA"
                     /locus_tag="ACHOQV_001440"
     CDS             10132..11799
                     /gene="ettA"
                     /locus_tag="ACHOQV_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFP9629836.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11855..12139)
                     /gene="nadS"
                     /locus_tag="ACHOQV_001441"
     CDS             complement(11855..12139)
                     /gene="nadS"
                     /locus_tag="ACHOQV_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP9629837.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPDFFRELASH"
     gene            complement(12141..12473)
                     /locus_tag="ACHOQV_001442"
     CDS             complement(12141..12473)
                     /locus_tag="ACHOQV_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9629838.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(12565..13797)
                     /gene="nadR"
                     /locus_tag="ACHOQV_001443"
     CDS             complement(12565..13797)
                     /gene="nadR"
                     /locus_tag="ACHOQV_001443"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFP9629839.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13818..15200)
                     /gene="radA"
                     /locus_tag="ACHOQV_001444"
     CDS             complement(13818..15200)
                     /gene="radA"
                     /locus_tag="ACHOQV_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP9629840.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(15249..16217)
                     /gene="serB"
                     /locus_tag="ACHOQV_001445"
     CDS             complement(15249..16217)
                     /gene="serB"
                     /locus_tag="ACHOQV_001445"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFP9629841.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            16323..16967
                     /locus_tag="ACHOQV_001446"
     CDS             16323..16967
                     /locus_tag="ACHOQV_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFP9629842.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16995..18011
                     /gene="lplA"
                     /locus_tag="ACHOQV_001447"
     CDS             16995..18011
                     /gene="lplA"
                     /locus_tag="ACHOQV_001447"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFP9629843.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            18043..18306
                     /locus_tag="ACHOQV_001448"
     CDS             18043..18306
                     /locus_tag="ACHOQV_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9629844.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(18467..19186)
                     /gene="deoD"
                     /locus_tag="ACHOQV_001449"
     CDS             complement(18467..19186)
                     /gene="deoD"
                     /locus_tag="ACHOQV_001449"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9629845.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(19243..20466)
                     /gene="deoB"
                     /locus_tag="ACHOQV_001450"
     CDS             complement(19243..20466)
                     /gene="deoB"
                     /locus_tag="ACHOQV_001450"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9629846.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20518..21840)
                     /gene="deoA"
                     /locus_tag="ACHOQV_001451"
     CDS             complement(20518..21840)
                     /gene="deoA"
                     /locus_tag="ACHOQV_001451"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFP9629847.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKNDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21918..22697)
                     /gene="deoC"
                     /locus_tag="ACHOQV_001452"
     CDS             complement(21918..22697)
                     /gene="deoC"
                     /locus_tag="ACHOQV_001452"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP9629848.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22955..24505
                     /locus_tag="ACHOQV_001453"
     CDS             22955..24505
                     /locus_tag="ACHOQV_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFP9629849.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24477..25340
                     /locus_tag="ACHOQV_001454"
     CDS             24477..25340
                     /locus_tag="ACHOQV_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFP9629850.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALWERG
                     VSRLIFPALYL"
     gene            complement(25558..26337)
                     /locus_tag="ACHOQV_001455"
     CDS             complement(25558..26337)
                     /locus_tag="ACHOQV_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9629851.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLKKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVLLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYTLFNVP"
     gene            complement(26334..27407)
                     /locus_tag="ACHOQV_001456"
     CDS             complement(26334..27407)
                     /locus_tag="ACHOQV_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFP9629852.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(27529..27690)
                     /locus_tag="ACHOQV_001457"
     CDS             complement(27529..27690)
                     /locus_tag="ACHOQV_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFP9629853.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27817..28422)
                     /gene="osmY"
                     /locus_tag="ACHOQV_001458"
     CDS             complement(27817..28422)
                     /gene="osmY"
                     /locus_tag="ACHOQV_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFP9629854.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28815..30404)
                     /gene="prfC"
                     /locus_tag="ACHOQV_001459"
     CDS             complement(28815..30404)
                     /gene="prfC"
                     /locus_tag="ACHOQV_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP9629855.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQSAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30495..31172)
                     /gene="yjjG"
                     /locus_tag="ACHOQV_001460"
     CDS             complement(30495..31172)
                     /gene="yjjG"
                     /locus_tag="ACHOQV_001460"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFP9629856.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(31187..31633)
                     /gene="rimI"
                     /locus_tag="ACHOQV_001461"
     CDS             complement(31187..31633)
                     /gene="rimI"
                     /locus_tag="ACHOQV_001461"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9629857.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31602..32015)
                     /gene="holD"
                     /locus_tag="ACHOQV_001462"
     CDS             complement(31602..32015)
                     /gene="holD"
                     /locus_tag="ACHOQV_001462"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFP9629858.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPIVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            32118..33149
                     /gene="rsmC"
                     /locus_tag="ACHOQV_001463"
     CDS             32118..33149
                     /gene="rsmC"
                     /locus_tag="ACHOQV_001463"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFP9629859.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLSDYAPLNKMDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT         7869 a         9131 c         8512 g         7893 t
ORIGIN      
        1 gttggattat taaaataatg tgacgaaagc tagcatttag atacgatgat ttcatcaaac
       61 tgttaacgtg ctacaattga acttgatata tgtcaacgaa gcgtagtttt attgggtgtc
      121 cggcccctct tagcctgtta tgttgctgtt aaaatggtta ggatgacagc cgtttttgac
      181 actgtcgggt cctgagggaa agtacccacg accaagctaa tgatgttgtt gacgttgatg
      241 gaaagtgcat caagaacgca attacgtact ttagtcatgt tacgccgatc atgttaattt
      301 gcagcatgca tcaggcaggt cagggacttt tgtacttcct gtttcgattt agttggcaat
      361 ttaggtagca aacatgcaga ccccgcacat tcttatcgtt gaagacgagt tggtaacacg
      421 caacacgttg aaaagtattt tcgaagcgga aggctatgat gttttcgaag cgacagatgg
      481 cgcggaaatg catcagatcc tctctgaata tgacatcaac ctggtgatca tggatatcaa
      541 tctgccgggt aagaacggtc ttctgttagc gcgtgaactg cgcgagcagg cgaatgttgc
      601 gttgatgttc ctgactggcc gtgacaacga agtcgataaa attctcggcc tcgaaatcgg
      661 tgcagatgac tacatcacca aaccgttcaa cccgcgtgaa ctgactattc gtgcacgcaa
      721 cctgctgtcc cgtaccatga atctgggtac tgtcagcgaa gaacgtcgta gcgttgaaag
      781 ctacaagttc aatggttggg aactggacat caacagccgt tcgttgatcg gccctgatgg
      841 cgagcagtac aagctgccgc gcagcgagtt ccgcgccatg cttcacttct gtgaaaaccc
      901 aggcaaaatt cagtcccgtg ctgaactgct gaagaaaatg accggccgtg agctgaaacc
      961 gcacgaccgt actgtagacg tgacgatccg ccgtattcgt aaacatttcg aatctacgcc
     1021 ggatacgccg gaaatcatcg ccaccattca cggtgaaggt tatcgcttct gcggtgatct
     1081 ggaagattaa tcggctttac caccgtcaaa aaaacggcgc tttttagcgc cgtttttatt
     1141 tttcaacctt atttccagat acgtaattca tcgtccgttg taacttcttt actggctttc
     1201 attttcggca gtgaaaacgc ataccagtcg atattacggg tcacaaacat catgccggcc
     1261 agcgccacca ccagcacact ggttcccaac aacagcgcgc tatcggcaga gttgagcagt
     1321 ccccacatca caccatccag caacaacagc gcgagggtaa acaacatgct gttgcaccaa
     1381 cctttcaata ccgcctgcaa ataaataccg ttcattatcg ccccaatcag actggcgatt
     1441 atccatgcca cggtaaaacc ggtatgttca gaaagcgcca gcaagagcaa ataaaacatc
     1501 accaatgaaa gccccaccag caaatattgc attgggtgta aacgttgcgc ggtgagcgtt
     1561 tcaaaaacaa agaacgctat aaaagtcagt gcaatcagca gaatggcgta cttagtcgcc
     1621 cggtcagtta attggtattg atcggctggc gtcgttactg cgacgctaaa cgccgggaag
     1681 ttttcccagc cggtatcatt gcctgaagca aaacgctcac cgagattatt agcaaaccag
     1741 ctgctttgcc agtgcgcctg aaaacctgac tcgctaactt cccgtttggc tggtagaaaa
     1801 tcacctaaaa aactgggatg cggccagttg ctggttaagg tcatttcgct attacgcccg
     1861 ccaggcacca cagaaagatc gccggtaccg cttaaattca gggccatatt cagcttcagg
     1921 ttctgcttcc gccagtcccc ttcaggtaaa gggatatgca cgccctgccc gccttgctct
     1981 aagccggtgc cgggttcaat ggtcagcgcc gttccgttaa cttcaggtgc tttcaccaca
     2041 ccaataccac gcgcatcccc gacgctaatc acaataaatg gcttgcctaa ggtgatattt
     2101 ggcgcgttga gttcgctaag acgcgaaaca tcgaaatcgg cttttagcgt taaatcactg
     2161 tgccagacct gaccggtata aatccctatc ttgcgttctt ccacgttctg attgccatca
     2221 accatcaatg actcaggtaa ccaaaaatgg ataaaacttc gtttccgctc cacggtttta
     2281 tcctcttcct gcaccgtata aagctcggtc acaggaatag cgatgagcgg cccaacgagt
     2341 ttttgcggcc cgctggtact ttggcgaatc gcatcttcca catcgctacg gtaatcagca
     2401 cgttcgacaa tcacctgccg aatcagcatt atcggaatca acaacagcaa tactgcacca
     2461 aacagggtgg tcattttcca gaacaggggg gatttcaaca tagtcttctc ctttgcaatg
     2521 gcagcaggat acgaaggcta tgtgggaaga atttgaagct atgtgaagtg acggtgaagt
     2581 cgaagcgaag ccagcacgcc accttcctgc acattgcgca gcgtgacttc gccgttaaac
     2641 aaacgggcga cctcactgac gaacgccaac cccagaccgc tgcttttttg cccatttgca
     2701 cgaggcaaag agtaaaagcg ttcaaaaata cgtgaaagcg cgtagtcagg aataccacta
     2761 ccggtatcca gcaccttaag cgtgacgtgt tcctgctcca cttcggcgct tagcgtgatg
     2821 caaccgctct cgggggtaaa atcgatggcg ttatccagta aattccccag agcctgctcc
     2881 agtaacgccg gttcagcagc aacgttaacc tcggtgggcg taacatgtag agtgatgttt
     2941 ttttctgcca actgcacggt gcgcgcttcg ctgacgcggc ggaataatgc cgccacatca
     3001 acagcagtta gaacgacttc ctgacgattc tccagtcttg cctggcgtag taacgtttct
     3061 accaatgcct gcatacgcgc attttgcgtc agaatgttgt cagtaaaacg agccaccact
     3121 tccggcggcg gaccttcgcg taaaatttcc gccgctccac gaatcgccgc cagtgggctt
     3181 tttagctcat gagttaatgc gtaaacatac tgctcaatat agtttttccc ttccagcttc
     3241 acgcgcatac tttccagcgc ctgcgcgagt ttacgcagct cgctactacc gagatcgggg
     3301 agaggaacgg gcttattgtc agtgacggaa tcagcatagc gagtgagccg ggcaatggag
     3361 cggttgatcc accaaaccat gcctgcgcca atcaccagtg caatccccaa caaaatggcg
     3421 ctggcccata aaattcgccg ctcgctgcgt ttaatgaccg gagccatcgc cgcgttcggt
     3481 ttgcctacgc tcaaaacgcc aataagccgc gagccgtcca taatcggcgc ggcaacatac
     3541 atcaccgaac tttcgggatc ggtaggattt tgcaacgtgc tgcgcgcacc atactgacca
     3601 cgcaacgtta gccagacgtc attccagcgc gaataatcct gtccaacggc tttatttgcc
     3661 gaatcgaaca atactttgcc ctgcgcatcg gtcatataga catgatattc attgcgcact
     3721 ttgttaatgc caccgatatt ggcgcgaaac gggcgatgct gtagctgatt aaacgcctgc
     3781 gccagttgcc catgcgttgt gtccccagag agcaaatcgg gacgcgccag ctccgccagc
     3841 aacgttgcgg tgtcgatcaa cgtcccctcc gttgctcttc gcacgcccgg tttaacttct
     3901 ttgacaaaaa tggccagtac gaaccaagct gccaccgcca ccagtaaaaa atagcccagc
     3961 aacaaccgca tgccgatacg cattacaggc ccctcaggct atatcccatg ccgcgatgag
     4021 tattaatcgg tgaaagatcg gggttgatgg cgcgcagctt ggcacgcagc gttttaatgt
     4081 gggtatcgac ggtgcgatcg taggtgtcct gcgcatcttc ccacacgcta tccatcagtt
     4141 gctggcggga ccagacgcgg cctggcgact tgagtaacgt cttcagcaat aaaaactcat
     4201 accgcgtcag cgttaatggc gtgtcaaacc agctgatctg cgccgcgggt tcattcaatt
     4261 caaaatgacc aatacggatg acgggagacg gcgtcgagaa cttcttcacc cgacgcagta
     4321 aggtgcgcac cctggcgcac acttcgcggg gtgaaaacgg tttagccaca tagtcgtcag
     4381 cgccaatttc cagcccaagc aggcgatcga cctcttcact tcgggcggtc aggaacagta
     4441 caggtaacgc cggatggagc gtcagtaact ggcggcacaa ttcaaagcca ctgatatcag
     4501 gcaaaccaac gtcgagaatc atgacatcgg gaacctgctg gcgagcttta tccagcaccg
     4561 gcaagcctcg ctcaaagacc tcgacggcaa aaccttcctg ctgcaacatg tagaccagcg
     4621 tgtcggctat cccttgctca tcttccacta accagaccgt ttcccgttgc ataaatcgcc
     4681 tctgttattg ccgccacggc atgacaggaa ccgcgctaat cgcgtttttc ggcgaacctt
     4741 ctacaacttt gtcggagtaa gccagataag cgagcgcgtt gcgtttagcg tcataaaagc
     4801 gcacgacctg taacgactta aagaccaggg acgtgcgttt tttgaatact acctcgccct
     4861 gagctttgcc gtttttaata cgatccgaca gttcaatcgg cccgacttgc tgacaagaaa
     4921 tggccgcatc ggaggtatct tctgccaggc ccaatccccc tttaatacca ccggttttcg
     4981 cccgactcac ataacaggtg acatttttca catcgggatc gtcaaaggct tccacaacaa
     5041 ttttgtgatc cgggccgatc attttaaata cggtgtcgac cgaaccaatc tcttctgcat
     5101 gagccaatgg ccccagcatt atcaggctta aagacaggat caaatgcttg tatttcatat
     5161 tgttaccatt tttatctatt acacgagcaa ttagttcgtc acggtaaata acatattgat
     5221 taagaacaaa atctcaatac ttttatttcc gtcaagccct aaaacatact ctactaagaa
     5281 aaaaacactg aatgctaaaa cagcaaaaaa tgctattatc caattacctg atgtcaggtg
     5341 ctcgttgttg aaaggatgag gatattttat ggatcaggcc ggcattattc gcgacctttt
     5401 aatctggctg gaaggtcatc tggatcagcc cctgtcgctc gacaatgtag cggcgaaagc
     5461 aggttattcc aagtggcact tacagagaat gtttaaagat gtcactggcc atgctattgg
     5521 cgcgtatatt cgtgctcgtc gtttgtcgaa atcggcggtc gcactacgcc tgactgcgcg
     5581 tccgattctg gacatcgcgc tgcaataccg cttcgactct caacagacat ttacccgcgc
     5641 attcaagaag cagtttgccc agactcctgc actttaccgc cgttctcctg aatggagcgc
     5701 ctttggtatt cgcccgccgc tacgtctggg tgaattcact atgccagagc acaaatttgt
     5761 caccctggaa gatacgccgc tgattggtgt tacccagagc tactcctgtt cgctggagca
     5821 aatctctgat ttccgccatg aaatgcgtta tcagttctgg cacgattttc tcggcaacgc
     5881 gccgaccatt ccgccggtgc tctacggcct gaatgaaacg cgtccgagtc aggataaaga
     5941 cgacgaacaa gaagtattct ataccaccgc gttagcccag gatcaggcag atggctatgt
     6001 actgacgggg catccggtga tgctgcaggg cggcgaatat gtgatgttta cctatgaagg
     6061 tctgggaacc ggcgtgcagg agtttatcct gacggtatac gggacgtgca tgccaatgct
     6121 caacctgacg cgccgtaaag gtcaggatat tgagcgatac tacccggcag aagatgccaa
     6181 agcgggagat cgcccaatta atctgcgctg tgaactactg attccgatcc gtcgttaacg
     6241 ctgcagctca tctaatgcag gggcgtccag atgcgagatg tcccctgcgg tttcaacgac
     6301 ccagccagac gccagccaca ggctttcctg ataatccacg cgcgaaatag aacagttacg
     6361 cagacgtaag cggcgctctg cccatgctgg taatccgaga atcgtactca ccagacatcc
     6421 cagtgcaata ccatggctca ccagcagcgg tcggcttccc tgcggtaagt cccggcaaga
     6481 ttccagtgcc gcattgacgc gatcgctgag ctcctgcatt gactcgcctt caggaatacg
     6541 cccgtcaacg gtgccattga ccagctgccg acgccagttc tcttcttctt cggtgagtga
     6601 atcgatattt cttgtttcca gtacacccat atttaattca cgcaggcgag aatcaaagat
     6661 gatatcacag ccgcaggcct gggcgatgat ttccgccgtg cgccgggtcc gtcctaaatc
     6721 gctactgatg atatgcgtaa tgccaagctc tttggcacgg gttgccacct gcatcgcctg
     6781 ttgctcacct ttggcggtca gcgggctgtc agactggccc tgaatacgtc gctcggcgtt
     6841 ccactgcgtt tcaccgtggc ggactaggta tacctgtaac atgctttttt tccgtatact
     6901 gctgtaacgt taattttctg agtaatgctg attatgcacc aagttgtctg tgcgaccacc
     6961 aatcccgcta aaattcaggc cattctgcag gcatttcacg agatcttcgg cgaaggatcc
     7021 tgccatattg catccgttgc cgtcgagagc ggtgtaccgg aacaaccctt tggcagtgag
     7081 gaaacgcgcg ctggcgcacg aaatcgggta gcaaatgccc gccgtttaca tccagaggct
     7141 gatttttggg tggcgattga agcaggcatt gatggcgaca gcaccttcag ttgggtggtg
     7201 attgaaaacg ccagccagcg cggcgaggcg cgttcggcaa ctttgccgtt acctgcggtg
     7261 attctggaaa aagtgcgtga aggcgaggcg ctcggtcccg tgatgtcacg ttataccggt
     7321 attgatgaga ttggtcgtaa ggaaggggcg atcggcgtgt ttaccgccgg gaaacttacc
     7381 cgcgccagcg tctatcacca ggcggtgatc ctggcgctga gtccgtttca taatgcggtg
     7441 tatcagtgat ctttcagcaa cacctcttcc agccactggc gcagctccac gggcgcggct
     7501 ttcaggctgt tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac
     7561 tcacgctggc tcatttcgcc gcgcaacagc tcttcgacaa tacgcacgcg agtccccaac
     7621 gcttcgcgct catctggcgt cagcatcagg tttaacaacg gtaaatggag atcgttttgg
     7681 taggcattct taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc
     7741 gctgctgaat agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa
     7801 aatgcctccc ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc
     7861 agtaacgacg tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc
     7921 ggtagtaagc gtcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa
     7981 tactctcgac aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca
     8041 cccgtccagg tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct
     8101 gataaacata ttgcaggtaa ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt
     8161 gcccagggct gctataacca ggaatagaga acatcttcac cgtatgggtc gccgtgcccg
     8221 gcataatctg catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc
     8281 tctcctgacg ggcaatcgcc atcgcatagc tttgcgggat ctccttccca ctggtgtaac
     8341 gtttgaaaag atcgttgtag gccagcggga atctctcttc cagatgatcc caaagcttcc
     8401 cggcgatcgt tgcctgaacg ctaagttccc accattggtt attgaaagca taccgagcca
     8461 gttgcgcctg ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg
     8521 tattatcgag attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca
     8581 gggcgctgtc aacattctgc ggcgctttat caatcttcag ctcatactct tcaccgatgc
     8641 gttgtgctgc aaccatcggg tagaaaccac gctgttgcat gagttgatgc aaaatctctt
     8701 ttgcttcagc ttcacgtccg cgttccagca gtaaatccgc ctgccagtaa cgccattcat
     8761 ctttctcttt cgcctccatc ggcaaacgcg ccagccaggt attcaggccg cgacgatcgc
     8821 cggtgccaag cgccattcgc acgcgccgtt caataagcga agtagattgc gaacgcataa
     8881 tggcgtcatc gcgccatttc gcctgctcat cggtgacatc attgcccatc aaacgccagg
     8941 cgacgatatc gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg
     9001 acgggatcat cagccgtgca ttttcagcat cctgccgcgc cacactggca aacgccaccg
     9061 ccgccatttg acgggtaaaa tcggtcgctc cggttgtacg cgcgaaggtc agtaccgtat
     9121 tagggttgtt cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct
     9181 gccctgccag cactgttacg agtccagtgt tacccgcttt catcgccaga cggatacgct
     9241 ctaaatacgc cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac
     9301 aggcgttagg ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt
     9361 cactctgccc ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg
     9421 gcttttccgg gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga
     9481 cgaaacgaga ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag
     9541 tgaccgtcac cgccggttga ttcatcaggt catcggtgat ctgacggtat tccaggtagg
     9601 gataaagcgg ataatctttc agtccaggca tcatttgttc gaccacatcc atttgtcgat
     9661 tatcccaggc ctgcttgatt tgggcgtagc ggctacgctg ctcatccagt gagtcggctc
     9721 gcgccacgct gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt
     9781 gtttggcttt ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc
     9841 gcgtaataga tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt
     9901 acttttatac tctaaataat tcgagttgca ggaaggcgat aagtgagtga gtccccagga
     9961 gcttacagaa gtaagtgact ggggtgagcg agcgtaagcg cagcacatgc aacttgaagt
    10021 atgacgagta tactgcgagg gtgatcgacc ggctgggatt agataaggaa acaggctaca
    10081 cttcgccttt gaaaactcat catcacgata aacatagagg cgaagtccaa cgtggctcaa
    10141 ttcgtttata ccatgcatcg tgtcggcaaa gttgttccgc cgaaacgtca tattttgaaa
    10201 aacatctctc tgagtttctt ccctggggca aaaattggtg tcctgggtct gaacggcgcg
    10261 ggtaagtcca ccctgctgcg cattatggcg ggcattgata aagacatcga aggcgaagcg
    10321 cgtccgcaac cggatatcaa gattggttat ctgccgcagg aaccgcagtt gaacccggaa
    10381 cacaccgtgc gtgagtccat tgaagaagcg gtttctgaag tggttaacgc cctgaaacgc
    10441 ctggatgaag tgtatgcact gtacgccgat ccggatgccg attttgacaa actggccgct
    10501 gaacagggcc gtctggaaga gatcattcag gctcacgacg gtcacaacct gaacgttcag
    10561 ctggagcgtg cggcggatgc gctgcgtctg ccggattggg acgcgaaaat cgctaacctc
    10621 tccggtggtg agcgtcgtcg cgtggcgttg tgccgcctgc tgctggaaaa accagacatg
    10681 ctgctgctcg acgaaccgac caaccacctg gatgccgaat ccgtggcctg gctggaacgc
    10741 ttcctgcacg acttcgaagg caccgttgtg gcgattaccc acgaccgtta cttcctcgat
    10801 aacgttgcgg gctggatcct cgaactggac cgcggtgaag gtattccgtg ggaaggtaac
    10861 tactcctcct ggctggagca gaaagatcag cgtctggcgc aggaagcttc acaagaagct
    10921 gcgcgtcgta agtcgattga gaaagagctg gagtgggtac gtcaaggtac taaaggccgt
    10981 cagtcgaaag gtaaagcacg tctggcgcgc tttgaagaac tgaacagcac cgaatatcag
    11041 aaacgtaacg aaaccaacga actgtttatt ccacctggac cgcgtctggg cgacaaagtg
    11101 ctggaagtca gcaatctgcg taaatcctat ggcgatcgtc tgctgattga ctccctgagt
    11161 ttctcgatcc cgaaaggggc gatcgtcggg atcatcggtc cgaacggtgc gggtaaatcg
    11221 acgctgttcc gtatgatctc tggtcaggaa cagccggaca gcggcaccat cactttgggt
    11281 gaaacggtga aactggcatc ggttgatcag ttccgtgact caatggataa cagcaaaacc
    11341 gtttgggaag aagtttccgg cgggctggat atcatgaaaa tcggcaacac cgagatgcca
    11401 agccgcgcct acgttggccg ctttaacttt aaaggggttg atcagggtaa acgcgttggc
    11461 gaactctccg gcggtgagcg cggtcgtctg catctggcga agttgctgca agttggcggc
    11521 aacatgctgc tgctcgacga accaaccaac gacctggata tcgaaaccct gcgcgcgctg
    11581 gaaaacgccc tgctggagtt cccgggctgc gcgatggtta tctcgcacga ccgttggttc
    11641 ctcgaccgta tcgccacgca catcctggat taccaggatg agggtaaagt tgagttcttc
    11701 gaaggtaact ttaccgagta cgaagagtac aagaaacgca cgctgggcgc agacgcgctg
    11761 gagccgaagc gtatcaagta caagcgtatt gcgaagtaat gcgtaaaatg ccggatgcgg
    11821 cgcgccagca tcgcatccgg tattagttaa atgattaatg ggaggcaagt tcccggaaaa
    11881 agtcaggatt ttctttaatt gtggcaagta cttttgcagc caatcccgta ggatttcttc
    11941 gcttcgattc ccagctttta atggtatcca cactggttcc cagagctttt gccatctcac
    12001 tttgcgaaac gttgagttgc tcacgaatag ctttaacatc cgcaatttcg tagcgggtca
    12061 cccgtgcagg ttcttttaaa ccttgtttaa tctcgacagc ctcttccaga gaggttttca
    12121 actcatcaaa aaaactcatt ttatatctcg cctttgagca gtttcgtcaa cttctttagt
    12181 tgagcttttt ccgtatctgt cagactatct ttggcatttt tcggataggc cataatcaaa
    12241 taaataatct cttccgtcgc cagaaagtaa attattctga cactgccact ttttccctgt
    12301 gaacccgcag ccattcggac tttacgcaat ccaccagtct gctgaatgag atcaccttta
    12361 tcgggagttc cgatgagttc tttttgtaat tcctttaatt catcatcggt tgcaatctgc
    12421 ttgatctgac gcgtaaacat tggcgtctct ataaatatta tcgccttacc cattacccct
    12481 ccctggaagg tacattgtac accaaaatgg agtactaagt acacttttga atcagtaatc
    12541 ccactcaaca tcttataaag atgattatct ttgctccccc atcatctccc gcaccagctc
    12601 cacgcagcgc aggaaacggc tgtcataatc gtcttcttca acccgcacaa attcgatatt
    12661 gttctcttcc agcatctcca ccagcaagtt ctggaactct ttgcgatcca ccgaactgcc
    12721 gaggctgcgt aaaccatccg ccacccaagg ggtgttgttc tccagcaaga taaccagatc
    12781 aaaacggtat tcatcgatca gcgcctgcac gaacggatgc tcccgccctt cgtacttttt
    12841 gcagaacgcc tgggtagtga caaaatcagt gtcgataaac gccactttat tggcatattt
    12901 cactgcaaaa tcaatgtatt gtgcgtgtcc cagcgcgatt ttatcgtagt cggaatactg
    12961 caatgcgatc tcatcaccgc cgaggtgtga aaagacataa tcgcgaccat attcccacgc
    13021 actggtggta ttgaagatat tggcaagttt gtttaccagg gtggatttac cgctcgactc
    13081 gccgccaagg atcgccacgg tacgcacaaa aaacggcttc acttcagtgg ggatatattc
    13141 ccagtagcgg aacgggtttt cgcggatctg cgcaccgctg atactcataa aggtgcgttt
    13201 cggatcgacc agcaccgttt cgatccccag atgttccata tactgtggcg catcggcttc
    13261 ttccgaggtg tagatcaggt ccggctggat ccccttttca gccataaact ttttgatgcc
    13321 gttgctccat acatcccagc cgtgcggata tggctccatg ccctcttcat tgaaagcatg
    13381 aatacggata tttttctgat atttaaaggt ttgcaataac cagcgcagac gatccggcac
    13441 cgtgggctgc tgcgacatgg cgctgtcttc gaacaacgcg cggtcgcggg tatcgtcaaa
    13501 gcccataatg atatgcagct cgtcaacctg gctacaggcg cgctggataa ggtagatatg
    13561 tccggtatgc agcgggtaga acttaccgaa tacgacacca atggttttct tctgtcgggg
    13621 aaattcaagc cccaaaaaac ggtgcaacgc ctccagcttt tgcgcgctgg ggcttttaat
    13681 tttggcattc agtaactggc ttaaataccc tttggtcata ccgctggcat cagcgacctg
    13741 ctgtagcgtg cagccctgtt gcttgatggc agttttcagg taatcaaatg acgacatatc
    13801 tccctccgta tctctcatta taagtcgtcg aacacgctaa gcgcgtcgga gagtttttta
    13861 acgccaaaaa tctgcatccc ttccggcgct tttttcggca cgttagccgc cggaacaatc
    13921 gcccggcgaa aaccgtgttt cgccgcttca gaaattcgtt cctggccgct gggcaccggg
    13981 cggatctccc ctgccagccc gacttcacca aacaccacca gatcctgcgg cagcggtctg
    14041 tcgcgcaggc tggaaaccat cgccagcagt aacgctaagt cggcgctggt ttccgttacc
    14101 ttcacgccgc cgaccacgtt aacaaataca tcctgatcgg ccatttgcaa cccaccgtga
    14161 cggtgcagca ccgccagcag gattgccaga cggttttgct ccagccctac tgcgacgcgg
    14221 cgtgggttcg ccatcatcga gtgatcgacc agcgcctgga tctccaccaa cagtggacgc
    14281 gttccttccc acactaccat cactgagcta ccggaggtca cttcatcacc gcggcttaaa
    14341 aagatggcgg aagggttgct aacttcacgc agcccctgtt cggtcatcgc gaagacgccc
    14401 agctcattca ccgcgccgaa gcggttttta tggctgcgta aggtgcgaaa acgagagtcg
    14461 gcatcgccat ccagaagcac cgaacagtcg atacagtgtt ccagcacttt agggccagcc
    14521 agcgagccat ctttggttac gtgcccaacc atgacaatcg ccacgccgcg cgttttggcg
    14581 aagcgcgtca gataagccgc cgtttcacgc acctgcgcca cgctgccagg cgatgactga
    14641 acatccgcca tatgcatcac ctggatggag tcaattacca tcagcttcgg ttgctcttct
    14701 tcggcaatca ggcagatctg ctcgatgctg gtttccgaca gcatattaag attgtcagtc
    14761 ggcaggccaa ggcgatgagc gcgcattgcc acctgttgca gcgactcttc gccagtgaca
    14821 tacagcgttt tcatctgctg cgccagtttg cacagcgttt gcagcaacag cgtggatttc
    14881 cccgcacctg ggttaccgcc aatcagaatg gcgcttcccg gcaccacccc gccgcctaat
    14941 acgcggtcga actctttaaa tccggtggaa aaacgcggca gctcctcaag gctgatgtcg
    15001 gaaagtttct ggacttttgc cactccggcg ctaccggcat agccactgag acgctcgtta
    15061 cgcgccaccg ttggcgacgc agcaagacgc acctcggtga tggtgttcca ggcatgacag
    15121 gcgctgcact gcccctgcca gcgcggataa tcggccccgc attcattaca aacaaaggcg
    15181 cgttttggag cttttgccac ggtgtacctc gttaatgctg tgccgcccgc aggatgacgg
    15241 gcaggcaatt acttctgatt caggctgcca gagaggatgc agaatacccc catcaggtca
    15301 gcgtgacgga tggtgacttc cgtcttttca ttcacttttg gcttggcatg gtaggcaatc
    15361 cccagccctg ccgctttgat catcggcagg tcattggctc catcgccaat cgccacggtc
    15421 tgcgccagcg ggatttcata ctcctgcgcc aggcgggtca gggttttcgc tttatattgc
    15481 gcgtcaacga tgtcgccgat cacattgccg gtaaatttac cgtccatgat ctccagttca
    15541 ttggctacca ctgctgtcag gcgcagcttg tcgcgcaggt attcagcaaa gaaagtaaag
    15601 ccgccggagg caatcgccac tttccagccc agcgtttcca gcttgagtac cagctgcgtt
    15661 aagcctggca tcagcggcag attttcacgc acctgttgca gaatattggc gtcagcgcct
    15721 ttcagcgtcg ccacgcggct gcgcaggctg gcggtaaaat cgagttcgcc gcgcatcgcc
    15781 cgttcggtta cctccgccac cctctcgccc gttccggcca gtttggcaat ttcatcaata
    15841 cattcaatct ggatggcggt ggaatccatg tccatcacca gcaaacccgg cgtgcgcagg
    15901 tgcgggattt tccccagcgg cgcgacatcc agctgcgctt cgtgggccag gcgcgtagcc
    15961 cgcgcggtga gtgaacctgc cagacgaatc acctgataat cttccacgca ccaggcggca
    16021 acaatcacca tcgccgcgcc cagtttgctc tggtattggg tcagacgttg tttatccagc
    16081 ccacgaccat acagcagcca gccgctacga cctgcgtggt aatccagtgg catcacttca
    16141 tcaccactta atgaaagagg cagacccggc cataaagaga catcttcagg caggtcgcac
    16201 caggtaatgt taggcattaa ggctcctgta aaatcgttcg aagcagggaa aataacgcat
    16261 gaggctacct tgtatccatt gcttctggca acattaagtc tcaaattttc aaagggtgga
    16321 agatggctcg cacaaaactg aaattccggc tgcatcgggc agtgattgtc ctgttctgtc
    16381 ttgccttgtt agtggcgctg atgcagggag cgtcatggtt tagtcaaaac caccagcgac
    16441 agcgtaatcc acagctggaa gaactggccc gcaccctggc gcgtcaggtg acgctgaacg
    16501 ttgcaccgct gatgcgtacc gactcaccgg atgaaaaacg cattcaggcg atccttgatc
    16561 agttaacgga tgaaagccgt atcctcgacg cgggcgtgta tgacgaacaa ggcgatctta
    16621 tcgcccgttc tggcgaaagc gtcgacgtgc gcgaccggtt ggcgctcgac ggcaaaaaag
    16681 cgggcggcta ttttaaccag cagattgtcg aaccaattgc cgggaaaaac ggaccgctcg
    16741 gctatctgcg ccttacgctc gacacccata cgctcgccac cgaagcccaa caggtggata
    16801 acaccactaa cattttacgc ctgatgttgc tgctctcact ggcaatcggc gtagtgctga
    16861 cccgcacgct gctacagggt aaacgcaccc gctggcagca atcgcccttc ctgttaaccg
    16921 ccagtaaacc ggtgccggaa gaggaagaaa gcgagaaaaa agagtgaccc attactacaa
    16981 gaaaggaaat cgttatgtcc acattacgcc tgctcatctc tgactcttac gacccgtggt
    17041 ttaacctggc ggtagaggag tgtatttttc gccagatgcc ggctacgcag cgcgttctgt
    17101 ttctctggcg caatgccgac acggtagtga ttggtcgcgc gcagaacccg tggaaagagt
    17161 gtaatacccg gcggatggaa gaagataacg tccgcctggc gcggcgcagt agcggtggcg
    17221 gcgcagtgtt ccacgatctc ggcaatacct gttttacctt tatggctggc aagccggagt
    17281 acgataaaac catctccacg tcgattgtgc tcaatgcgct gaacgcgctc ggcgtcagcg
    17341 ccgaagcttc cgggcgtaac gatctggtgg tgaaaaccgc cgaaggcgat cgcaaagtct
    17401 caggctcggc ctatcgcgaa accaaagatc gtggcttcca ccacggcact ttgctgctca
    17461 attccgacct tagccgcctg gcaaactatc tcaatccgga taaaaagaaa ctggcggcga
    17521 aaggcattac ctcagtgcgt tcccgcgtga ccaatctgac cgaattgcta ccggggatca
    17581 cccatgagca ggtttgcgag gccataaccg aggccttttt cgcccattat ggcgagcgcg
    17641 tggaagcgga aatcatctcc ccggacaaaa cgccagactt gccaaacttc gccgaaacct
    17701 ttgcccgcca gagtagctgg gaatggaact tcggtcaggc tccggcattc tcgcatctgc
    17761 tggatgaacg ctttacctgg ggcggcgtgg aactgcattt cgacgttgaa aaaggccata
    17821 tcacccgcgc ccaggtgttt accgacagtc tcaaccccgc gccgctggaa gccctcgccg
    17881 ggcgactgca aggctgtctg taccgcgcgg atatgctgca acaggagtgc gaagcgctgc
    17941 tggttgactt cccggaacag gaaaaagagc tacgggagtt gtcagcgtgg atggcagggg
    18001 cggtaaggta actcgctgat taaaatatcc agggaaggaa atatgatcga tctggaaaac
    18061 caggaacggg agattatcaa tttaatgttc agccagggta tatcctggct aaccgccgtg
    18121 cgtatcaggc ataaactatc acttgctgaa gtaagtaaaa tgttaggtat ctccattaat
    18181 tctctaaaac agatcgaaaa gacggaacgg cttagttcaa acatcaaaag caaaatggca
    18241 ggaatttatg gttgtccacc agagttgctg atatgccctt cctggatgac ggctgaacat
    18301 aaatgaatag attcgtgaat agatacttga atggatttta ttttctatcc atatgaaaaa
    18361 taatagtaaa tctataaatc agttgctatt gatgaataaa tactaaaaag ccggagcagt
    18421 ctcccactcc ggcttcacac gacaatcgcc tttcagcaaa acacaattac tctttatcgc
    18481 ccagcagaac ggattccagc gcgattttga tcatgtcgtt gaaggtggtc tgacgctcag
    18541 cggcagtggt ctgctcgtga gtgcggatgt ggtcagacac ggtgcagatg gtcagggctt
    18601 tcgcgccaaa ttctgcagcg acgccgtaga taccagccgc ttccatttcc acgccgagga
    18661 tgccgtattt ttccatcacg tcgaacattt cgccgtccgg agagtagaac aggtcagcgg
    18721 agaacaggtt accaacgcga gcatcaacgc ccagtgcttt tgccgcgtct accgcgttac
    18781 gcaccatgtc gaagtcagcg atagcggcaa agtcatggtc tttaaaacgg atgcggttaa
    18841 ctttggaatc ggtgcaggca cccataccga taacgacgtc acgcagtttt acgtgcggca
    18901 gaacagcacc acaggaacct acgcggataa ttttcttcac gccgaaatcg gtgatcagtt
    18961 ctttggtgta gatggagcag gacgggatac ccataccgtg acccattacg gaaattttgc
    19021 ggcctttata agtaccggtg aagcccagca taccgcgaac gttgttcact tcacgggcat
    19081 cttcaaggaa agtttcagca atatacttcg cacgcagcgg gtcgcctggc atcaaaacta
    19141 cgtcagcaaa atcgcccatt tctgcattaa tgtgtggagt agccatcgtt ttatcctttt
    19201 tgtgacatat taaaggcgga gtcactgctc cgcccaaatc catcagaaca tggctttgcc
    19261 atattccata tcagaagtac caaaatattt tgccagagtc tggccgatat ccgcgaaggt
    19321 ttcacgatgc cccagcgaac ctggtttcac tttcgggccg taaaccagta ccggaatgtg
    19381 ttcacgcgtg tggtcagtac cggtccaggt cgggtcacaa ccgtggtcag cagtgagaat
    19441 cagaatatcg tcatcgcgca gcaaagacat cagctccggc agacggcggt cgaacagttc
    19501 cagacccgca gcataaccgg cgacgtcgcg acggtggccc caggaagagt cgaagtcaac
    19561 gaagttggtg aagacgatgg tgttatcacc cgcttctttc atctctttga tggtggcgtc
    19621 aaacagcgcg tccaggccgg tcgctttcac ttttttggtg ataccgcagt tggcatagat
    19681 gtctgcaatt ttaccgaccg aaaccacctg gccgtgtttt tcatcaacca gtttctgcag
    19741 cacggtcggt gctggcggct caacagccag gtcgtgacgg ttaccggtac gctggaagtt
    19801 accggctttg tcgccgataa acggacgagc gataacacga ccaatgttgt agccgccgtt
    19861 ggtcagctct tcacgggcga tttcgcacag ttcgtagagt ttatccagac caaaggtttc
    19921 ttcgtggcaa gcaatctgga acacggagtc agcggaggta tagaaaatcg gcttgccggt
    19981 tttcatgtgc tcttcgccca gttgatccag aatgaccgta ccggaagagt ggcagttacc
    20041 gaggtaaccc ggcagattag cgcgttcaac cagtttatcc agcagctctt gcgggaagct
    20101 gttttcgtga tcggagaaat atccccactc aaacagaacc gggacaccgg cgatttccca
    20161 gtgaccagac ggggtgtctt taccggatga catttcgtgc gcccatgcgt acgcgccgat
    20221 aacttcagcg ttgccatcca ttcccgccgg aatgaaaccg gtagaacctt cgtgtgcttt
    20281 cgccagccca agacgggtca gatttggcag attgagcggg cctttacgac cgtggtcagc
    20341 ttcgcctttg gcacaagctt ctgcgatatg acccagggtg tcagccccga cgtcaccaaa
    20401 gcgttctgca tcttctgtag cgccgatgcc gaatgagtcc agcaccataa taaatgcacg
    20461 tttcatatgt tctccgtact cagtgcttca aatttaaatg atcagatcag tataccgtta
    20521 ttcgctgata cggcgataga cagttggtgt gctttccggt gctttatcgg caagtttaat
    20581 tgccgctttc accgctttcg ccgcttcctg ccagctgttt tcgtctttcg cgtggataac
    20641 tgccagcgga cgctgaccgt ctacctggtc gcccagacgc gccatatcag taaagccgac
    20701 gctgtaatcg atggtgtcag atgcctgacg gcgtccgccg cccattgcaa ccactgccat
    20761 ccccagtgcg cgggtatcca tttcactgac aaacccttcg gtatcagcat agactgcttt
    20821 cgtcagcatc gctgtcggca gatacttcgc gtagttctca acgaagtcgg tcgggccttt
    20881 ttgtgccgcg accatacgac caaagacttc tgccgcttta ccgttgtcca gcaccgcctg
    20941 caatttcgcg cgcgcttcgg cgtcattttt cgccagtttg ccggagatca gcatctccac
    21001 gcacagtgcc atcgtgacat caaacagacg cgggttacga tattcacccg tcaggaactg
    21061 caccgcttca cgaacttcaa ccgcgttacc tgcactggag gccagtacct gattcatatc
    21121 ggtgagcagc gcggtggtgc gcacgccagc gccgttagcc acgccaacaa tcgcttcggc
    21181 aagggcttca gagagttcgt aggtcggcat aaacgcgccg ctacctactt tcacgtccat
    21241 caccagcgcg tccagacctt ccgccagttt cttcgccaga atagaggcag tgatcagcgg
    21301 gatggagtcc acggttgcgg taatatcacg ggtcgcgtag aaacgtttat ccgccggagc
    21361 cagtgagctg gtctggccga taatcgccac gccgacgtct ttaataattt cgcggaaacg
    21421 gttgtcatcc gggaaaatgt cgaagccagg gatggattcc agtttgtcga gcgtaccgcc
    21481 agtatgaccg aggccgcgac cggagatcat cggaatatag ccgccgcagg ctgcgaccat
    21541 cggccccaac atcagcgaag tgacatcgcc gacgccgccg gtcgagtgtt tatcaacaat
    21601 cgggccattc agatgcaggc ttttccagtc gagaacggtt cctgaatctc gcatagccat
    21661 ggtcagcgag acacgctcag gcattgtcat atcgtggaag aaaatggtca tcgcgagggc
    21721 ggcaatctgc ccttcggaga tagtgttgtc gcgaataccg ttgataaaga aacgaatttc
    21781 ttcatcgctc agcgcatgac catcacgttt tttacgaata atttcttgtg cgagaaacaa
    21841 ggtaacctcc cgtggaaaag agacgaggta atcggggaac cggatgagca tcgctgcatc
    21901 atccggcaaa tcaagcctta gtagctgctg gcgctcttac cgtcgccgtg gcccagagct
    21961 ttcagcaggc ttgccagcag gctggaagca ccaaagcggt agtgacgcgc atcagcccag
    22021 tcagcaccga acagttcatc ggcgatggcg agatatttct gcgcatcttc cgcagtacgc
    22081 acgccgcccg ccggtttgaa accaacggtt ttttctacgc ccatatcacg gatcacttcc
    22141 atcatgatgc gcgcgctttc cggcgtcgcg ttcacagcca ctttaccggt agaggttttg
    22201 atgaagtccg cacccgcttt gatggagatt tcagacgctt tacggatcag cgcttcgtct
    22261 tttagttcgc cggtttcgat gatcactttc agcagtacat tcgctgccgc gcaagcctct
    22321 ttacaggctt tcaccaggtc aaaaccaacc tgctcgttac ccgccatcag cgcgcggtac
    22381 gggaacacaa cgtcaacttc atcagcaccg taggcgattg ccgcacgggt ttctgccagc
    22441 gcgatttcga tgtcgtcgtt accgtgtggg aagttggtta ccgtagcgat acggatttcc
    22501 ggggtaccct gctctttcag cgttttgcga gcaattggga taaagcgagg atagatacag
    22561 atagcggcgg tattgccgac cggagttttg gcctgatgac acagagcaat tactttctcg
    22621 tcggtgtcgt cgtcattcag ggtggtcagg tccatcaatt tcagtgcacg caggctgctt
    22681 gctttcagat cagtcatttc attctcctgg cttgtcgcca ataaaattca ccttgcgagt
    22741 ttgttagtat tctaacatct actccgcaac acacttcgat atacatcaca attaaggaaa
    22801 tctacttaca agtttgcatc actgtaatgc gatctggttc aaataattca ctttcaaatg
    22861 aatgcgtcag tggtggcaaa cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa
    22921 tggcgcatca ccttcggcat aaaaggaaaa gaagatgccc acttctcatg aaaatgcact
    22981 gcaacaacgt tgccagcaaa ttgtcaccag cccggtactt agcccggagc agaagcgcca
    23041 ttttctggca ctggaagcag aaaacaatct gccttaccca cagcttcctg ccgaagcccg
    23101 ccgcgcgctg gatgaaggtg taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc
    23161 gcgctatgtc ttacccgatt acgcccgttt tctggcgaac ggttccgaat ggctggagct
    23221 ggaaggcgcg aaagatcttg atgatgcact ctctctgctg accattcttt accaccacgt
    23281 accatcggtc acatcgatgc cggtctacct ggggcaactg gatgcgttgt tgcaaccgta
    23341 tgttagaatt ctaacacaag acgagatcga tattcgaata aaacgtttct ggcgttacct
    23401 cgacagaacc ctgccagacg cctttatgca cgccaatatc ggcccgtctg attcgcccat
    23461 tacccgcgcg atcttacgtg cagacgcaga gttgaagcag gtttcaccga acctgacctt
    23521 tatctacgat cctgaaatca cccctgatga cctactgctg gaagtggcga agaacatctg
    23581 tgaatgtagc aaaccgcaca tcgccaacgg tccggtgcat gataaaattt tcacaaaagg
    23641 gggctacggg attgtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct
    23701 ggtacgcctt aacctgaaag ccattgccga gcgcagtgaa tcgctggatg acttctttac
    23761 acgcactcta ccgcactact gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt
    23821 cctctatcaa caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa
    23881 ccctgaacgt tttgtgccaa tgtttggcat gtacggattg gcggaagcag ttaacttgct
    23941 gtgtgagaaa gaagggattg ccgcgcgcta cggtaaagaa gccgccgcaa atgaagtggg
    24001 ttatcgcatc agcgcacaac tggcggagtt tgtcgccaat acccccgtga aatatggctg
    24061 gcaaaaacgc gccatgctac acgcacagtc ggggatcagt tccgatatcg gcaccacgcc
    24121 tggcgcacgc ttgccgtatg gtgatgagcc agatccgatt acccatctgc aaactgtcgc
    24181 cccgcatcat gcttattatt attccggcat cagcgacatt ctgacgctcg acgaaaccat
    24241 caaacgtaat ccgcaggcgc tggtacagct ttgcctcggt gcctttaaag ccggaatgcg
    24301 tgaatttacc gccaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtgcg
    24361 tttgtcggat ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg
    24421 agaagaagcc gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga
    24481 acagcagatg cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggca
    24541 gtcgtctggc tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt
    24601 ggacgatggg gcgctgtaat gattgcggag agtgcgtgcc gcagtgtccg catcaggcgt
    24661 tgcagattgt cgacggcaaa gtggtgtgga acgctgtggt ctgcgagcaa tgtgatacct
    24721 gcctgaagat gtgtccgcaa catgccacgc caatggcgca gtccatgagc gtggatgagg
    24781 tgcttagcca tatccgcaaa gcagtgctgt ttatcgaagg gataacggta agcggcggcg
    24841 aagccacgac ccagctaccg tttgtggtgg cgctgtttac tgctatcaaa aacgatccgc
    24901 aactgcgcca cctcacctgt ctggtggaca gtaatggcat gttgagcgaa accggctggg
    24961 aaaaattgct tccggtgtgc gacggcgcaa tgctcgatct caaagcgtgg gggagcgaat
    25021 gtcatcagca attaaccgga cgcgataatc agcagatcaa gcgcagcatc tgtttgctgg
    25081 cagagcgcgg taagctggcg gaactgcgtt tgctggtaat ccctggccag gtggattatt
    25141 tgcaacacat cgaagaactg gcggcgttaa tcaagggact tggcgatgtt ccggtacgcc
    25201 tgaacgcgtt tcatgcccac ggcgtgtatg gcgaggcgca aagctggccg agcgccacgc
    25261 cggaggatgt ggaacaactg gctgatgcgt tatgggagcg cggggtgagc cggttaatat
    25321 ttccggcgct atatttgtga catgccggat acggcctacg gatcggcaca gacctgtagg
    25381 catgataaga tgcgtcaagc gtcgcatcag gcattgtgcg ccagctgccg gatgcggcgt
    25441 gaacgcctta tccggcctac ggttcggcac agacttgtag gcatgataag acgcgtcaag
    25501 cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt atccggccta
    25561 cggcacgtta aacaacgtat acgtattatt tagcaacact tcggcaatct catcttctgg
    25621 ttccgggcgt aactcgcaaa gtacgtcaaa cacccgcgca gcctgttccg ggcggttagg
    25681 ctgcccctga aagccgttga gcggcatatc cggcgcgtcg gtttccagca acaacgatgc
    25741 cagcggtaat tttgcgatga catcgcgggt tttactggcg cgtggatagg tgatagtacc
    25801 gcctacgccg attttgtagc ccagcagtac aaacctttcg gcctgttgca ggctgccgga
    25861 aaaaccgtga accacgccag tgcgtggcaa atcgtggcgt ttaagatgca tcgccagttt
    25921 gtcgtgtgtg cgccgtgaat gcaggatcac cggcagatcg tagcgtttcg ccagtttcag
    25981 ttgttcgtcg agtaaccact gctgcctctc aaattgcgga tcgtcgccga agagatccag
    26041 accgatctcc cccaccgcca ccaccttcgc cggacgcctt tccagcgcct gctgtagctg
    26101 atcaagagac acatcgctat gttttttcaa cataccagga tgcaagccca atgcggcata
    26161 cagcggttga tatttttccg ctaacgcctg cacgcgggca aaattttccg cctctgtcgc
    26221 cggaacaatg atcttgccta cgcccgcttg tgccgcgcgt tgcaggctgg cctcttcatc
    26281 accactaaaa ggcgggaaat caaaatggca gtgggtgtcg ataaaacggc aaatcaagcc
    26341 agatcctcat tgttaaatgt cgggtcgttc gcctgcggag cgtcgatcac ttcggcaacc
    26401 agcgtatcgt tggcgactgg cgcaggcgga atgacaatcg attccggcgt caccaccggc
    26461 accagatggc gggtaagcgg cgctttttca gtcaataact taccgaccgt tgccagaaaa
    26521 taacgaccgc ataaacgccc aagtttatag tcttcacgca gcgccggaat ccgactgcca
    26581 agcgcgatac tatgcaacgg cttcggcgga taaatttcga atatccgcag ctggcccggt
    26641 ggtttctcaa taaattgctg aatgtcacga tagctggttt catgatgctg caccagattg
    26701 accagcggct gcaggctact gtcacccagc cagcgttcca tgcgtttgaa ccactgcggc
    26761 gtgtagtaca tttgcgacgg cacggtgcgg atgacgacca aggtttttgc tccctgcctt
    26821 gccgcctctt taaccggaat cgcatcactg atcccgccat ccaggtagtt aatgccttcc
    26881 agcgacactc cgctacgata aaagccaggt atcgcactgg aggcgcgaat cacatccagc
    26941 cagttttgtt tggttggtaa aaagtaattc ggcgcatagt catcctgacg acaggcgcac
    27001 atataaaacg atttgccgct gtcaaacaac cgcgcggcgg tatccatttg caacggcatc
    27061 tggcttgcag tggcctccac cagccagtcg agatcgataa gatttcctcc acggacaaag
    27121 cgcaatggat cgaaaaattc gcgttttgtg gtatagcgca tgatgacttt gcgcgcgtaa
    27181 ccgggctgat tgcagatata cgccgagagg ttctgcgccc cggcagatgt gccgagataa
    27241 agatcgaaag gattaaactg cgcgcgcata aactcatcca gcacgccagc cgtgaaaatt
    27301 ccacgctgtc cgccgccttc gcacaccaga gctattcgtc caggctggaa cggccttagc
    27361 gacaacggcg caatattacc aagcgttaca ggtattcgct gccccacctc tgccttcctg
    27421 tttttgttgt gaaagaatga caaagtaacg caatttatta ttcgctaaaa ccgcaaaagc
    27481 cagtccgctg gactggcttt ggataaaatt ttcttaatgg tctgaaatct agggtcgttt
    27541 tcggcccatg aacaaactca ccaggaacag aataatcccg acgacaaaga caattttagc
    27601 tgcgcctgca gcggtaccgg ccagaccacc aaacccaagt gcggcggcga ttaacgcgat
    27661 aaccagaaat atgatgcccc aacgaaacat aagcgtctcc tttaccatag ttaatgtcac
    27721 cgcttgatat gagcgtgaaa atcactcaat gcgatatttt tagtgtggtg cacatcgcgc
    27781 ctcccgacaa agttcgggag gacgaattac gacgaattac ttagttttca gatcattttt
    27841 aacgcttttc acaccatcta ccgctttggc gatactttca gcacggtcgc tctgtgcctg
    27901 ggaatcgacg gtaccggaga gctgaaccac gccgtcggtg gtttcaactt tcacatgacg
    27961 ggaagggacg atatcgtccg ccagcagttt ggctttgatt tcactggtgg tggcggtgtc
    28021 acccgcgtag cccttcaccg agccttcttt agcgtcgcga acgtgcagtt tgtcgctgac
    28081 agaggtcacc ccttcaacgc ctttcgccac tttcactgcc tcttcggcct gagcctggct
    28141 ttcaacgaaa ccgctcaggg tcacgacttt ttgatcggtt ttgacagaga tatcggtgct
    28201 cttgatgttg tcatgatcca ccagcgccgc cttcactttc gcggtgatgg cgctgtcatc
    28261 catgaaatta ccgactttat tcatagagct atcgactttt tgccctgcgc tttcattggt
    28321 agtctgtgcg ttgttttccg cgtaggcaga gccggtcgcg acggcagagg tcaacattac
    28381 agccagcaga gttttcgaaa tcttcagtct tgtcatagtc atcgatttat tcctgtatgt
    28441 ttgctcgtaa tttgagcctg gcaacacgag gttgcattgc tgaataggga gagacttcac
    28501 cctctacaga agtcaatggc cgccatcaca aaagcgatgg gtgatgaata acgaccatta
    28561 cagcctctga atcagttatt aatatcggta gaatgacaat cgacggcttt agatactgat
    28621 atctacgcat tgaacggtat ttaacgccgt cagaaatgtc attactttgt taaatataga
    28681 tcacaatttt gaaaccgctc gggatatcag cgaaaacata agcaaaagtg aatgttttaa
    28741 gaacattccg taagcggcta ataaggaagg gaaattgaca gggcgcagcg gctaccgcgc
    28801 cctggaggca agaattaatg ctcgcgggtc tggtggaact gaacgtctgg ataacgttcc
    28861 tgcgccaggc gcaggttgac catgctggta gcgatgtaag cgaggttatc gccgccatca
    28921 agcgccagtt ggctttcgtt cttacgcttg aactcttcga atttcttcgc gtccgcacat
    28981 tctacccagc gggcagtggc aacgttgact gactcataca ctgcttcaac gttgtattcg
    29041 ctcttcagac gcgctaccac cacatcaaac tgcagcacac caaccgcacc aacgatcaga
    29101 tcgttgttgg agatcggacg gaacacctgc accgcgccct cttcggaaag ctgtaccagc
    29161 cctttaagca gctgtttttg cttcagcgga tctttcaggc ggatacgacg gaacagttct
    29221 ggcgcgaagt tcggaatacc ggtgaacttc atcatctcac cctgggtaaa ggtgtcgccg
    29281 atctgaatgg tgccgtggtt gtgcagacca aggatatcgc caggatacgc ttcttcaacg
    29341 tgcgaacggt cacccgccat aaaggtcagc gcgtcggaga tcaccacatc tttcgcagta
    29401 cgcacctggc gcagcttcat gcctttttca tatttaccgg acaccacacg cataaacgcc
    29461 acgcggtcgc ggtgtttcgg gtccatgttg gcctgaattt taaatacgaa gccggtaaat
    29521 ttgtcttcgc tcgcctctac ggtacgggta tcagtctgac gcggcatcgg cgcaggagcc
    29581 cactccacca ggccatccag catatgatca acgccgaagt tacccagcgc agtaccgaag
    29641 aataccggag tgatttcgcc cgcaaggaac agctctttgt cgaactcgtt agacgcgcct
    29701 ttcaccagct ccagttcgtc acgtagctgc tgtgccagat cttcaccaac cgcagcgtcg
    29761 agatccgggt tattcagccc tttaacaatg cggacttcct gaatcgtgtg gcctttaccg
    29821 ctctgataga gataggtttc atctttataa aggtggtaaa cgcctttaaa cagcttgccg
    29881 cagccaatcg gccaggtgat cggtgcgcag ccgattttca gctcgttctc aacttcatcg
    29941 agcagctcca tcgggtcgcg gatatcacgg tcaagtttgt tcataaaggt gaggatcggc
    30001 gtgtcgcgca gacgggtaac ttccatcagc ttacgggtac gatcttcaac accttttgcg
    30061 gcgtcgataa ccatcaggca gcagtccacc gccgtcaggg tacgataggt atcttccgag
    30121 aagtcttcgt gccccggggt gtcgagcagg ttaaccaggc aatcgtgata cggaaactgc
    30181 atcacagacg tagtaatgga gatcccacgc tgcttttcca tctccatcca gtccgactta
    30241 gcgtgctggt tggaaccacg gccttttact gtaccggcgc tctgaatggc ctgtccgaac
    30301 agcagcacct tctcggtgat ggtagtctta ccggcgtccg ggtgagaaat aatggcaaaa
    30361 gtgcggcgct tcgccacctc ttgcaaataa ggagacaacg tcataattct ttcttcttga
    30421 gtaagcggca tcgcgccgcg cttgttgaaa acgaaaaatt gcggctattt tacccatcaa
    30481 cgggggggag gcaatcagtg tttacacagg agctgctcca gttcgtgcaa cgaagaaacg
    30541 gtccaggtgg gcgcgatgcc ttctggttgc tcgcgattgt gcgcattcag ccagcaggtc
    30601 gcaagcccgg cgttgatgcc accgagaata tcggactcag ctgtgtcgcc aaccatcagc
    30661 acacgtgaac ggtcaggatt gcctgcctgt tccagcgcat aatcgaaaat tttcttattc
    30721 ggtttggcaa cgccaacttc ttcggaaatc accagcaaat cgaaataatc acgcaggccc
    30781 gtgcgttcca gacgcacttg ctgcaaggcg ctaaagccgt tggtgatgat gccaatttta
    30841 gcgttgccac gaatggcgtt aagcagagaa accgcgcccg gcagtggcgt acagatttcc
    30901 gccatcgcat taataaaggc ttcgttgagt ttacccggct cgacgttcag ccgttcggcc
    30961 cagctctcaa accgcccgtg ctgaagctgt aatgaagtga tcgcgccgtt ttgataatcc
    31021 acccacagtg gcttgttaac ggcctgatag tcctgaaaat cttcagcggt aaaggtgacg
    31081 ctgtaatcaa gaaacatccg ctgcaagccg gtgaatgagt caaaggtaaa cagcgtttca
    31141 tcggcatcaa agaaaatcca gtcccacttc attattccac cttgtcttac atactgattg
    31201 gcaacgccat gatgatggcg tcttcgcgac cgtccgtggt ggggtagtaa ttgcggcgaa
    31261 ttgtcgcctc gttaaagcct aaactttcgt acagggcaat ggcggcagcg tttgaagcac
    31321 ggacttccag ccatagcgtc gccacgccgc gtttttccag ttcgtcgatc agatgttcca
    31381 gcagcgccct tcccaatccc tgacgctgat agtcaggatc gaccgcaata ttgaacaatg
    31441 tagcttcatc cagcaccact tgcgtaatcg caaacgccgc cattttgccg ttttgcgtta
    31501 actgaaagtt gagataacgc tcgccctggt tgctggcaaa ggttttttca ctccacggaa
    31561 aggcgtgggc gcgttgttca atgtggtaag ccgctggtaa atcagtcgtt tcgagggaag
    31621 aaatcgtgtt catatgtgca aatttgttgc cataacgcgg cgcgtgccgt tgggtttgcc
    31681 cgtaattcgg tgagcgccgg tgatgccacc tgagcgcctt ccagtgatag cggttcgtca
    31741 gtacccaacc gccagctgtt gcagcgactg ccttgcggca gcatcgcgat tttttctggc
    31801 gtcagttgca gcacttgatc ggggctgacg gttaacgcgc gcagaacatc gctcactata
    31861 ggatcggtca gggcgggaag atcgtttgcc accatcacca gacggacgtg tgccgggatc
    31921 gcaatggcaa tctccccctg caacgcgcca gggcgacgca gcgaccactg ggtaatgccc
    31981 agttgctgta actgccagtc tcgtcgggat gtcatagcga atcgctcctg ttgtcagggg
    32041 cgcaaatata gcaaattcgt cgataccgcg ccaacatatg gctataatcg ccgccagtat
    32101 taattgagga gcattccatg tctgcattta ccccggcaag tgaagtcttg ctgcgtcaca
    32161 gtgatgattt cgaacaaagc cgtattcttt ttgccggaga cttacaggat gacctgcccg
    32221 cgcgtttaga taccgcggcc agccgtgctc atacccagca attccaccac tggcaggtgt
    32281 taagccgcca gatgggggat aacgcccgtt ttagtctggt cgccacggcg gatgatgtcg
    32341 cagattgcga tacgctgatt tactactggc cgaagaacaa accggaagcc cagttccagt
    32401 tgatgaattt actttctctg ctgccagtgg gcacggatat ttttgtcgtt ggcgagaacc
    32461 gcagcggcgt gcgcagcgcc gagcagatgc tgtcagatta tgcgccgttg aataaaatgg
    32521 acagcgctcg tcgctgtggc ctctattttg gtcgtctgga aaaacagcca gtatttgatg
    32581 ccgataaatt ctggggcgaa tacagcgtcg atggcctgac ggtcaaaacg ctgcctggcg
    32641 tgtttagccg cgacggtctg gatgtcggta gccagttgct gctctcgacg ttaaccccgc
    32701 acacgaaagg taaagtgctg gatgtcggct gtggcgcggg cgtactttcg gttgcctttg
    32761 cgcgccattc gccgaaaatt cgtctcactc tgtgcgatgt ctctgcacca gcggtagaag
    32821 ccagccgcgc aacacttgcg gccaacggtg ttgaaggtga agtctttgcc agtaacgtct
    32881 tttccgaggt gaaaggtcgt tttgatatga tcatctccaa cccgccgttc cacgatggga
    32941 tgcaaaccag cctggatgcc gcgcaaacgc tgattcgcgg cgcggtgcgt catcttaata
    33001 gcggcggcga gctgcgaatt gtagcgaacg ccttcctgcc ttatccggac gtgctggatg
    33061 agacatttgg cttccacgaa gtgatcgcgc aaaccgggcg cttcaaggtg tatcgcgcca
    33121 ttatgacccg ccaggcgaag aaaggttgag tatctgatat atgtcgccgc ataccgtgtc
    33181 ggatgcggcg tacacgcctt atctgaccga caaacgaatt atccacaatc cgctgatgga
    33241 catttttcca gcaattacac ctctgtcgat aattaactat tgacgaaaag ctgaaaacca
    33301 ctagaatgcg cctccgtggt agcaattctt tttaagaatt gacggtatgc gaaggtggcg
    33361 gaattggtag acgcgctagc ttcaggtgtt agtgtcctta cggac
//
LOCUS       DAZKGF010000031        32993 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZKGF010000031 DAZKGF010000000
VERSION     DAZKGF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.031"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(383..673)
                     /gene="nadS"
                     /locus_tag="ACHOQV_001464"
     CDS             complement(383..673)
                     /gene="nadS"
                     /locus_tag="ACHOQV_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP9629860.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(674..985)
                     /locus_tag="ACHOQV_001465"
     CDS             complement(674..985)
                     /locus_tag="ACHOQV_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9629861.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1214..2122
                     /locus_tag="ACHOQV_001466"
     CDS             1214..2122
                     /locus_tag="ACHOQV_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9629862.1"
                     /translation="MAQEKGIAELVEEFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEAMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAELVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2186..3127)
                     /gene="fabY"
                     /locus_tag="ACHOQV_001467"
                     /gene_synonym="yiiD"
     CDS             complement(2186..3127)
                     /gene="fabY"
                     /locus_tag="ACHOQV_001467"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFP9629863.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3172..3609)
                     /gene="dtd"
                     /locus_tag="ACHOQV_001468"
     CDS             complement(3172..3609)
                     /gene="dtd"
                     /locus_tag="ACHOQV_001468"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP9629864.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDCA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3606..4478)
                     /gene="yihY"
                     /locus_tag="ACHOQV_001469"
     CDS             complement(3606..4478)
                     /gene="yihY"
                     /locus_tag="ACHOQV_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFP9629865.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4472..5071)
                     /gene="yihX"
                     /locus_tag="ACHOQV_001470"
     CDS             complement(4472..5071)
                     /gene="yihX"
                     /locus_tag="ACHOQV_001470"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFP9629866.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5212..6099)
                     /locus_tag="ACHOQV_001471"
     CDS             complement(5212..6099)
                     /locus_tag="ACHOQV_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP9629867.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMTSSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(6089..7438)
                     /locus_tag="ACHOQV_001472"
     CDS             complement(6089..7438)
                     /locus_tag="ACHOQV_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9629868.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(7537..8970)
                     /locus_tag="ACHOQV_001473"
     CDS             complement(7537..8970)
                     /locus_tag="ACHOQV_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9629869.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(9040..10050)
                     /locus_tag="ACHOQV_001474"
     CDS             complement(9040..10050)
                     /locus_tag="ACHOQV_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFP9629870.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQQLKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(10083..10970)
                     /locus_tag="ACHOQV_001475"
     CDS             complement(10083..10970)
                     /locus_tag="ACHOQV_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFP9629871.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(10995..11873)
                     /gene="yihU"
                     /locus_tag="ACHOQV_001476"
     CDS             complement(10995..11873)
                     /gene="yihU"
                     /locus_tag="ACHOQV_001476"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFP9629872.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNTQAVDALVTQG
                     ATAAQTPAEAAASAEFIITMLPNGDIVRQVLLGEKGVCETVSSDALVIDMSTIHPLQT
                     DALIRELQEKGINMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            12046..12942
                     /locus_tag="ACHOQV_001477"
     CDS             12046..12942
                     /locus_tag="ACHOQV_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9629873.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            12982..13770
                     /locus_tag="ACHOQV_001478"
     CDS             12982..13770
                     /locus_tag="ACHOQV_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9629874.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFTA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(13830..15095)
                     /gene="yihN"
                     /locus_tag="ACHOQV_001479"
     CDS             complement(13830..15095)
                     /gene="yihN"
                     /locus_tag="ACHOQV_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9629875.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(15197..16177)
                     /gene="yihM"
                     /locus_tag="ACHOQV_001480"
     CDS             complement(15197..16177)
                     /gene="yihM"
                     /locus_tag="ACHOQV_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFP9629876.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEEGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNN"
     gene            complement(16185..16895)
                     /gene="yihL"
                     /locus_tag="ACHOQV_001481"
     CDS             complement(16185..16895)
                     /gene="yihL"
                     /locus_tag="ACHOQV_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9629877.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTRDIPETLVPLITVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(17112..18935)
                     /gene="typA"
                     /locus_tag="ACHOQV_001482"
     CDS             complement(17112..18935)
                     /gene="typA"
                     /locus_tag="ACHOQV_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFP9629878.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRSPKDD"
     gene            19307..20716
                     /gene="glnA"
                     /locus_tag="ACHOQV_001483"
     CDS             19307..20716
                     /gene="glnA"
                     /locus_tag="ACHOQV_001483"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFP9629879.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLA
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            20890..21939
                     /gene="glnL"
                     /locus_tag="ACHOQV_001484"
     CDS             20890..21939
                     /gene="glnL"
                     /locus_tag="ACHOQV_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFP9629880.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            21951..23360
                     /gene="glnG"
                     /locus_tag="ACHOQV_001485"
     CDS             21951..23360
                     /gene="glnG"
                     /locus_tag="ACHOQV_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFP9629881.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            23472..23582
                     /gene="yshB"
                     /locus_tag="ACHOQV_001486"
     CDS             23472..23582
                     /gene="yshB"
                     /locus_tag="ACHOQV_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFP9629882.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(23772..25145)
                     /gene="hemN"
                     /locus_tag="ACHOQV_001487"
     CDS             complement(23772..25145)
                     /gene="hemN"
                     /locus_tag="ACHOQV_001487"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFP9629883.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(25334..25843)
                     /gene="yihI"
                     /locus_tag="ACHOQV_001488"
     CDS             complement(25334..25843)
                     /gene="yihI"
                     /locus_tag="ACHOQV_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFP9629884.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(25962..26128)
                     /locus_tag="ACHOQV_001489"
                     /pseudo
     CDS             complement(25962..26128)
                     /locus_tag="ACHOQV_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26425..27057
                     /gene="yihA"
                     /locus_tag="ACHOQV_001490"
     CDS             26425..27057
                     /gene="yihA"
                     /locus_tag="ACHOQV_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP9629885.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(27381..30167)
                     /gene="polA"
                     /locus_tag="ACHOQV_001491"
     CDS             complement(27381..30167)
                     /gene="polA"
                     /locus_tag="ACHOQV_001491"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP9629886.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPVAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVVDEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            30531..31463
                     /gene="yihG"
                     /locus_tag="ACHOQV_001492"
     CDS             30531..31463
                     /gene="yihG"
                     /locus_tag="ACHOQV_001492"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP9629887.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCESLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPISDELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(31504..32934)
                     /gene="yihF"
                     /locus_tag="ACHOQV_001493"
     CDS             complement(31504..32934)
                     /gene="yihF"
                     /locus_tag="ACHOQV_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFP9629888.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMRGNVIPALAAAKVKLVDNELTQPLFIAAKNKSPMEATLRFAFGGSFSTTLDVAP
                     AKYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDLVLELSPLNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQINIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFVIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLIFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8309 a         8459 c         7893 g         8332 t
ORIGIN      
        1 tttttgattg gatctttatt tgcataatct gaatggtgta gccgaaaaat gtcagcatcc
       61 taacagcacc cggatttaag gtgaaattaa tcatccatca cagaccggtc agtactgcta
      121 ccaaccggtc ttaacttgct catcgttata attttgttgg taacagttcg ggaaacaagg
      181 aattccttcc ggaacacccc cggtatcatc cgatagcatt gacgtcaatc atcggcgcga
      241 cgatgagttt ttttaatcgc atcatagggt ggcaacggtc agcacgatta cgggtcatag
      301 caaaactcct gagcaggtgg cactacgccg ctttcacagg agttctgaat gctggtgctt
      361 tctgtatggc ataaccatgc ctctaacggt taagtgcttg cagcacatgt tgcggatcat
      421 tggcaatcgc tcgcagcaaa gcctttgcag gcccggtcgg tgaacgtcgc ccctgctccc
      481 agttacgcaa cgtgtctacg cttacagaaa taagatcggc aaatttagat tgtgacaacc
      541 ctgaagcgcg gcgtatttct ttgattttca tagcatcaac ttcaaaagtc cgcgaaggct
      601 gtcgcgttcc ttcaataatt tcattcatct gggccatgct gtgagtcaac cgctcaaaaa
      661 gtgccttatc catttaccac ctcgtattta acaaccggag cattgctttt tcctggggag
      721 acagatcatc tttaataccc ttacgataaa taagcagtaa tctgatctca taatgtttaa
      781 cctgatgaaa gtaaataacc ctgacaccag cacgtttccc ctttcctcct gacacccatc
      841 gaacctttct taacccacca gtttcaggta ttacctcacc atagtcagga ttcaaggcca
      901 ggaaaaactg aaaacgactg aattcatcgt cggtcagcag tttttgaaca tcttcagtaa
      961 aaatttccgt ttcgatgaag agcataatta tacgccattg gcttagagta acaatccttt
     1021 ttgtacgtca ttgacgtacc cttgaaacca ctagcttact gatagtgctc cagcaaaacc
     1081 acgtctctgc cgggtttgtc tttacatttg cgaggcgctt tccagatttt tcacgcccgt
     1141 gaatttccct gtatccatcc agtaaaaacg ggtgtataaa actgtacatt catacagcaa
     1201 aatgagttct actatggcac aggaaaaagg aattgcagag ttagtcgaag agtttatcgc
     1261 tgcgggtcgc ccctcatcgc gtcaacaaag tattgcgcag cgcagggagg gctatatcgc
     1321 cagcgctgtc cttgcgggtg agacagaaac gcgggtagat attcagacta tcgaacttga
     1381 agcgatgacc ttacgaatag tgtcaccgct gaatgccccg acattactcc cgacgataat
     1441 ttattactac ggtggctgtt ttgttagcgg cgggttcgca actcacgaca accagctgcg
     1501 ccagttagcc tattacgggc aatgtcgggt tattgccgtc cagtatcggc tggcaccgga
     1561 acatactttc cccgccgccc atgatgatgc gcaacgaggt gcagaattag tctggcaaca
     1621 cgccgaacga ctcggcgttg ataaacaacg gataactctt gccggtgaca gtgcgggcgg
     1681 acatctggcg ctggtaactg ctttgcgatt aaagcgtgct ggcgagtggc aaccggcaca
     1741 actcatctta atttatccca tgcttgatgc gactgcgcat ttcgaaagct acatacgcaa
     1801 tggtcacgat tacatcatca cacgcgacac tttactgagc ggctttgaga tgtacctgcc
     1861 aggcattgaa cgcaggcatc ctgaagccag ccctctttgg cgcgatgatt tcaatggctt
     1921 gccacctgta catatcatta ccgctgaata cgacccactt tgcgatgaag gtgaagcgtt
     1981 gtatcaccgc atgacagggc aaggagtgca atgcacctgc caacgctacc ttggcgttat
     2041 ccacggattt tttcagttag gtggtatcag tgaggctgcg cgatcggcgc tacgggatgt
     2101 tgcctggaga gcgggaaggt aactccggac cagggcccag aaaaggtttt cagattgaaa
     2161 accttttcag tcaagtaaaa cgcccctact cttcttcgtt cccgccctct tcatacgggc
     2221 caaatggctt cgcgggcaga acgatatacg tgccttcaaa cactgcaccc ggcgtctcgt
     2281 cgccaaagat ttctacttgc atctgcaccc gtgcttttcg tccgcgcgcc agacggtcga
     2341 gatcgccgct taaggcacca aggtcggcta ccgcatgagg tttgccgcta atcggcttgc
     2401 tgtagcggat atgcgcatcc gccagaataa tcgttccgcc gaggtggcgt tcgcgcagca
     2461 tcagccagat aagcccccaa ccggtgagcg ttgccagcga gaataaactc ccggcaaaca
     2521 gcgtgtgatg cggattctga ttgcctgttt ccggcatggt ggtgataaat ttttgcccgg
     2581 tatattgctg aatgcgcacg cccatttttt cactcagcgg gatgtgttca taccacgcct
     2641 gttgcagctg cgcgcaccag tcgccgcgat gcaaaatatc atccagagtg gcaacgggtt
     2701 taatcatcaa aaaatggcga atcggcgtgg tggttggcgt ggtgatttct ccctgattaa
     2761 caaaccccag cttggcgaaa aactccaccg cgtcttcgcg ggcgctacag gtcacgcgct
     2821 taacaccttc ctgacgcgcc acagattcga gggtcatcgc catcagcgtt cctaaccctt
     2881 tgtcctgcac gtcgggatga acggccataa agcgaatgga cgcttcattg tcggcattaa
     2941 tatacagtcg gcctaccgcc accagattac cctgctcgtc gacgaccatc tggtgatgcg
     3001 ccatcgcatc ccacgcgtcg cgttccgaac cttttggttg atgcaggggc ttacgcaaca
     3061 tttcccagcg aaactgatag taacgctcta attcttcttc tgtttgtgga acccgaaggt
     3121 gatacatagc gatactctct cttgtttccc gtgacaaccc tggaagctgg ctcatacctg
     3181 caaccagaat gtcacggggc catcattgac cagcgatacc tgcatatccg cagcgaagcg
     3241 tcctgtttgc gtgttcatct cttgctgacg gcagcgttcg acgaaatagt catataacgc
     3301 ctctgcgcaa tccggtgatg cgcctttgga gaaacttggg cgcatccccc gttcggtatc
     3361 tgcggcaagg gtaaactggg aaaccaccag tacactgccg cccgcctgtt gcacgttgag
     3421 attcatcttg ccttcggcat cgctaaagat gcggtagccg agcacacgct cgcacagacg
     3481 gtttgctttc tgttcgtcgt catccttttc gacacccaat aacaccaaaa gtcccgcgcc
     3541 aatttcaccc gtcacttctc cctccacggt gacgctggca cgggttacgc gttgaattaa
     3601 tgcaatcatg gttcgtcgtc ttcttcttgt tcagcagctt gttttaattt gcggtattcc
     3661 ccgagagtga cagtaatttc cgcgccaagc aagacgatac accatgtcca gtagacccaa
     3721 acaaagagaa tggggatcac cgccagcaca ccgtaaatga gctgatatga tgggaacatg
     3781 gtgatataaa gcgcgaaacc tttctttcct gcttcgaaca ggagcgcggc gacaaacgcg
     3841 ccgacaatcg cgtcgcggtt aggtacacgg atggtaggaa cgatgctgta cagcagccag
     3901 aaagagatcc acgacaacag cagcggaaaa atacgcaaca cattatcgat gacagtattg
     3961 agatcgctcg cccagcgcag agagagcaga taggagctga tcgccagact ggcccctgcc
     4021 aacagcggcc ccagcgttaa aatcatccag tacacggcaa acgagtaaat ttttggtcgc
     4081 gctcgtttac tgcgccagat ggtattcaac gcgctatcga tggagtacat caacaataac
     4141 gccgtgacga tcagcccgca cgccccgacg gcggtcattt tgttggaatt ggcgacaaac
     4201 tgttcgatat atcgctggat aacatcgcca gtagcaggca gaaagttggc aaaaataaag
     4261 tgacgcaact gaatgctgac gtcggaaaac atgggaaaag cggcgaaaag cgcaaaaaca
     4321 acggcaacca gcggcactaa tgagagcaac gacacatagg caaggttacc tgccagggtt
     4381 gtcatgttgt cctcatcaat gcgttgccag agtagtttta gccaggccca tagtggacgg
     4441 gtacgatgcc tggctttgtc ctgaatggtt tttagcataa caccttcgcg aaatagtccg
     4501 ggatggtggt tttatctttc accagaatac tagtaatgcc tagctgatta gctccttcta
     4561 tattatcggc gttatcgtcg aaaaagaccg tatcgtctgg tgaaaaacct tctgcctgca
     4621 aaacatgctg ataaattcgt gcttcaggtt tgcgcatacc caagtcttgc gacagataga
     4681 tatggtcagc agcatcacgg atttccgggt attcctccgg ccagaaggta gtatgcaggc
     4741 ggttagtatt ggaaagcacc accacgcgat gcccctgctc acgcagttta tgcatgatgg
     4801 cgatcacttc cgggcgcagc gcaacaaata ccgcctgcca accgtgggag aactgctcat
     4861 agcttagcgg cagggccatc tcatggcaca gtgcctctgc gaacgcttcg tcgctaattt
     4921 ccccacgctc atgctgatga aacgcctcgc ccatgtggaa actcttctta agcgtagcca
     4981 gtggaacacg cgttaaatcg ctccaggccc ccagcacacg gttaaagtcg atatcgacaa
     5041 tcacattacc taaatcaaag atatagagca tttttgcctc ctttcgcgcc acaagaaatt
     5101 aactgtagcg ggaaaggatg ggtttggcta tgtggcaata agggtaatct tgtgatgctg
     5161 attgcgaaaa agcagagtaa tttcactggc tttttcgcaa tagatgaatg gttaagattc
     5221 aggtgtaaat tgccaacgag tttcgatgac cagcggctta tctggtgtca caaattccgg
     5281 cgttagattt attgcgttag gtgcctgagt cagcgggtta atacaggtgg catcgggctg
     5341 tttatcaaat accaccagac tgtttgcaga agaggtcatg gtcatcgcga gtttaccagg
     5401 ccacagtaac ttaaccttca ccccgtcata aaaaccaaaa cagtcatccc agggaccgac
     5461 ttgaggagag atacgatttc cagttggcag ttcatcacta cccgcttctt cttgccagtc
     5521 cgcatcaaaa agcacctgca acgattctgt attttgcgga gtcagctttt tcgcaaacca
     5581 cggatgccag cctgcggagg ccgggaatgt atcggcatat gaatggactt ccagttgcaa
     5641 aaccagcgca tcgttctcca gcaaaaaagt ctgaatcact tccccttgcc agggccacgg
     5701 tgaagccagg ggcatacgta aagtcaggga atctgctgtt ttgtcgatga tttcccacgt
     5761 tgagtaacaa gccataccat gcaacgcatg tggtggttta ttggcgggta aagaataaca
     5821 ttgcccacca gcattcagcg tcgcattccc cagtcgccct gcccacggca ccattgggaa
     5881 acagccatac tgaaaagctc tgcgctgcgg ctgccattgg cgtaatactt cagaaccaaa
     5941 tgcctttaac gaggttatcc tggcaccatc atgcggataa atctgcaggt gaatattgcc
     6001 tttttccagc gtcactaact ctcctacagg cccaggccat ggcgcgtcaa acgcctgttg
     6061 tagcgccagg taatgctcgt tttttcgttc agttttcatg agattgttcc ctgatgtagc
     6121 ggtaagaagc ggtttcttgt tcatcggttg gctcaatcag ccagacgcaa acaaaggaaa
     6181 gaactgagac agcaaataaa aacagagcga cataccacgg ttttcctccc cctagcgcca
     6241 gcaaacttga ggcgataagg gggcttagtc cgccaagaat cgcggaaaat tgataagcaa
     6301 aagaaaggcc ggtatatcgt actttggtac cgaacatgcg ggtgaaaagt gttggctgca
     6361 cggcaaacat catcgtcgga cctaagttgt agccaaggat catactgcac cagatgatga
     6421 gcgtactttt gctatccagt aataagaaga aaggaaacgc gaacaggaca caaaatcctg
     6481 ctcccgacaa atacagactt ttttgaccta tacgatctga taaccatcca accaatggac
     6541 aactgaaaat accgatcgcc gaggcaatca acatacccgt caggggaata tctcgcgaca
     6601 atgctaattg gctggaaata tagacaatac caaaggcgtt aataatatta gaggagacac
     6661 tttccgccag cctcgcaccc agtgccaata aaatatttcg tggatgtttt ttgaataatt
     6721 caatcaacgg aggaacggac ttttctttat tattcgtcgt tttttgcttt tgaaaatcca
     6781 gcgtttcttc ggtatgcagg cggataaaca gcccaacgat caacatcacc gcagaaagcc
     6841 aaaacggaat gcgccatccc caggagagaa actgctccga agtaagaaaa tggttacaga
     6901 gagcaaaaat accggttgcc agcataatac ccacagacgc agccgtttgc gggagtgaac
     6961 ctaaaaatcc gcgtcgggat tcgggcgctg attctatggt catcaatgcc gctccgccgt
     7021 actcgccacc aagaccaaag ccctgaatca aacgcagcac cattaataca agcggtgccc
     7081 aaatgccaat ttcctgatac gtcggaataa aaccaatcaa aaatgtggag caaccgacaa
     7141 tagccagcgt ccagataagt gttatttttc gcccgatttt atcgccaaaa tgaccaaaga
     7201 cgatgccacc taaaggacga gtcagaaaac caacaccaaa ggtcgcaaag gaagctataa
     7261 gacctataaa aggatcgctg tctggataaa acagcggtgc aaataccagt cctgctgccg
     7321 tgccaaaaat aaagaagtca taccattcca ttaacgcgcc tgcgaaactg cccataacga
     7381 cacgtctcat ggctttaccg gaaagccctt cacgcggtgc gggtaacttt tcagacataa
     7441 ttttcctctc ctggtaggcg agaaatctgg gatacgaacc actaaacgtg ggcagtttga
     7501 attaatgcgc atcggcttaa accgatgcgc attaaattat ttcttcaatt cagccagaac
     7561 ctctgcgtcc tcagcatcaa catttgcacc cagtggttcc caattaatca atacacaggt
     7621 caggaatccc aggaatggca cgattgccaa ccagtaaaac gcttcagttt tcaacgcacc
     7681 ccacagcatc ggccagaaaa tgagtcctgc aattgcccct gtgcgcataa tagcgcgggc
     7741 aaatccgacc ccagtagggc gaatagcggg aggataagag agtgtggcaa tggtcatgcc
     7801 cagcccacca ggacccgctg aatggaagaa aataattgat cccaataacc ctaaagacag
     7861 ccagggatta gtcgttgcca gtgcgcctaa cgacagcaat gctaaggtca caacagcaaa
     7921 tccatacatc gactgccggc gagtacccag tcgttggaga ataaacgagc cgatcatccc
     7981 gcctgtcacc ccacaaaggg cattcacgac ggcagaaccg gttaacgcgc cagtaagccc
     8041 gccacttatt attcccgcca acgtaagagg aagataaaca cctacggcgt tatattgcca
     8101 cgcctgcatg gttgccacga cacagccaag aatggtgcgt ttgcggtaac gatcattaaa
     8161 taagatgcca tatccaccac tgtatttatt ctctgctttt tccgagaatt tcgcttctgt
     8221 tgtacccgat gccgcaacgc gagcctgaac accataacgt tttcccagaa tgtcgcacgc
     8281 ttcctggtaa cgcccgacgc gtgcggccca cattgcggat tcaccaataa agaagtaacg
     8341 caaaatcatg aagatgactg caaaaatagc tccgatgaat attccgtaac gccacaattg
     8401 ttcttcagcg atacccgaca gaagcagcgg caaaagcacc aaataaacaa cggttgtcga
     8461 aacataccag gccatttgcc agaggttgac cgacgtgccg gttttcttcg acgttgaacc
     8521 acgaagctct gccacggcat tggttgccaa agggaagtcg atgcctaaac caaagcccat
     8581 gatgacgcgg cacaccagca cccaccagat actgggagca atagctaccg ctgcagcacc
     8641 gatggtgcac agccccatgc caataataaa tgcccgcttt tgcccaaagc ggtttattac
     8701 tcggttcgct aataaaccgc caattaacgc ggcgattaat actgatgcat tgacggttgc
     8761 agcaagaccc ggcgaaatgc cgaattgagc ggcaatgtat ttattaccaa accccaccat
     8821 cgccgcctgg taggcatcaa tcaatatgcc gcccaaagca ataaaaataa cgccgatact
     8881 ggtgttgatt gcagagttct tattaacaat atcaattacg tcctgtggcc gactaattga
     8941 ataagttgca ggactggtga tttgactcat cgcagtgtcc tttttaattg ttgtagggtg
     9001 gcgctgacgt gacgcagcgc cagacagaga tttagaaaat cattccggcg caaattgcag
     9061 catgaccttt atggagtcat tttgctgcgc gcgttcgaat gccgccgctg catcctcaat
     9121 ggcaaataaa tcggtaacca gttcactggt atcaaaccgc ccatccgcca gccattcgat
     9181 aacagcatcg aaatcttcag ggatgtacat gccagagttg agcaagtcac gctcgaaacg
     9241 ttgcatcatc ggtaatggta tttcacgcgg cccacttggt acgcccatgc acacgacggt
     9301 gcctccggca tatacacggg tacatgcatc gttaagcgtg gcctgcgctg caacgacatc
     9361 aatcgccccc gtaaatcgca catctgcggc cagctcacct ggtgcccaaa cttctgctgc
     9421 gcccagtttt aacgccagcg cgcgtttggc agcgtcaggt tcaatcacgg taatcttacc
     9481 tgcccccata atgcgcagtg cctgcacgat ggataagcct atcgttccgg caccaatcac
     9541 cagtacgctt tccagagaag ctttcggcat tcggttaacg cagtgacgag cacaagccgc
     9601 cggttcggca aaggccaaca cttttagcgg taaagaggct ggtgcgacat ggcaattacg
     9661 cgcaggaaca atgtgttgtg agcgagcaaa gcccggtgcg cggaaaccag caacctgcgg
     9721 tggttcacaa agattaaaac gtcctgcttt gcaggctcgg cattccatgc aagccatgat
     9781 gggatcgacc acaacatgat cgcccggctg tacattttta acgtcgctgc ccacttccgt
     9841 aacgcgcgct gcaatttcat gaccggggac gacaggcggt ttggcaaacg gatgaccgcc
     9901 tttcagaaca tgcagatccg aaccgcatat cccgtagaac acgggcgcaa tccgtacttc
     9961 gtgcggttta agttgctggt gtggtacttc aacatattga gtggtgactt tcccaatatc
    10021 tgaaaataaa acctgggtaa ttttatccat tgttattctc cagaatcgat atccataggg
    10081 gattagcgtt gcgcgatacc ttcatcgaca atttcagcca gccgttgcaa gcgtggaacg
    10141 gagacatcgc gcagcatggt ttgtggatct tgtgcaccaa caaccgacgc ccaaaccgct
    10201 cgtccagcca ggaagcccga cgcaccacct ttcatggcaa tacttactgc acggccaaaa
    10261 atatcggcat caacgccaga agaaagaatt acccacggca tcttcatctg atcgttaagt
    10321 tgctgacagg cagcaagtaa ggttttttca tcgcctttgc cccctaatgg catctcggct
    10381 ttgtaaagat cagcctctgt tccaccaagc tctgcggcgg cggcaacaat ggcgctttca
    10441 cgatcaaagt cccaaccgcg acgaggtgga cgcaccacag gttcaataat gctaaccagc
    10501 ccggcactac ggcagcgacg aacaaattta tcaaccattg ccagacgttc ctctgcgggt
    10561 tcatcttcac gccagagtac aagtaatttc attgccttag cccccatttc acgggcttta
    10621 tgcgggtcga cggacatatc tatttcaacg ctatcaaccg ggatgccatt acctgggata
    10681 aaaagatcgg cagcgacaat caggccgcaa gaattagcca cagcgccctg ctcgactatt
    10741 tgttcaagac aaaattgttt atccgccaga acggcagaag cataaggaga aaggattcgg
    10801 gtcgcggcaa ctttaaaatc ggtgagtaca ctgtcggcga tgggttttgg ctgccctgct
    10861 gccgcaaaca tcaggcgcat ggcctcacgc tgatcgacag ctaacatggc gaatccacca
    10921 cctgggcggg agatatcttt cagcgtaaat ggggtgaagt gggatgacat agaaactcct
    10981 gttaatagat aaaattaaag accagcctgc tggcgaacct gatttagcag ggcgctccag
    11041 tcctgccgcc ctttgccgtc ttgacttgct gcggcgtaat aactttctgc tgcaatcccc
    11101 atattgagcg gcgcccccgt ttcccgagcg acgtcaactg caatacgcag atctttcaat
    11161 gccaggtcca ccatgaaggc gggagaaaga tcgcctttca gcactttgcc aggccaggtg
    11221 gtcgtaaaat gtcctttccc tgctgccgta ccactcatca ccttaattgc gacatcaaga
    11281 tttaatccca gtgactcaca taacacagcg gcttcggagg acagcgcatt aagcgcaatg
    11341 ctcatatagt tgttaattag cttcacgcgg atccccattc ctgggccacc agcctcgacc
    11401 agctcattac ccatgcacat gagaatcggg cgtgcacgcg caatctgctc atcagtgccg
    11461 cccgctaaaa tcagcaatgt gccatcaatc gcattcactg acgttctgcc cacgggggcg
    11521 tccatcatat tgatgccttt ttcttgtaat tcccgtatta atgcatcggt ctgtaaggga
    11581 tgaatagtcg acatatcaat cactaacgcg tcagatgaaa cagtttcaca gacgcctttt
    11641 tcccccaaca gcacctgtcg aacaatgtcc ccattgggaa gcatagtgat gataaattca
    11701 gcgcttgctg ccgcttccgc tggggtttgt gccgcagtgg ccccctgcgt aactagcgca
    11761 tcgacggcct gtgtattgac atcaaatacc tgtaatgaat gccctttttg caatagattg
    11821 ttagccattg ggctacccat ctgccccagc ccgagaaatg caatggttgc catgaatact
    11881 ctcctgtctg acattgacaa aataatgaca attgaaaatt cgttttcgtc tgtttttgag
    11941 catatttgta atttatgttc aaaaacacga cacgcatcac taatttgatc atttgtgtca
    12001 tttaaattga ccaaagacga caaaattaaa caggacaaga caaatatgac cagaattgca
    12061 tgcgtgggta tcacagttca ggacaggatt tattctttgc ccactctgcc ggagggcggc
    12121 gggaaatatc aggcaaatca ttatcttgag attggtggcg gcccggcagc aacagccgct
    12181 gttgcaatcg caaagcttgg cgtggaggtt gatttcatcg gtcgcgtcgg cgatgacagt
    12241 tgtggaaata ccttacttgc agaacttgag ggttggggcg tcaataccgc attttgccgc
    12301 cgatatccta acgcccgttc gtcgcaatca gcgatcctcg ttgatcagca tggcgaacgg
    12361 attatcgtta attatccaag tcctgatctc ggtactgatg ccgaatggtt agaagcgatc
    12421 gatttctctc gttatgatct gattctggcc gatgttcgct ggcatagcgg tactgaaaaa
    12481 gcattctcgc tagcgcgcct tgcgggtgtg acaacgcttt tagacgccga tatgacaccg
    12541 caggatattt ctccacttgt cgcgcttgcc gatcacgccg tgttctcaac acctgggctg
    12601 aaacgcatga ctggcctgca gtcacctgaa gaaggtttgt tccaggcaac gacacaaaca
    12661 gcaggtaaag tgtatgttac gctgggttct gaaggatcgt tgtggattga agatggtcat
    12721 ttgtgtcaac aggaggcatt ttctgtcaat gtggttgata ccactggagc aggcgatgtt
    12781 tttcacggcg cactcgcggt agcgttggca gaaaaaatgc caacaaaaga ggctattcgt
    12841 tttgccagtg cggttgccgc gatgaaatgt acccagcctg gcggtcgggc cggcattcct
    12901 aatcgtgagc aaaccgaatc atttttgtca ctttatgcgt aaaatggtaa acatctaccg
    12961 aggaggggca agggagtatt catgagtatc atcgaagtga caggtaatcc acgccatgac
    13021 caactcgttc atctaatcgc agaacgcggt tatatgaata ttgaagaact ggcacaactg
    13081 ctggatgttt caacgcaaac cgtgcgccgg gatatccgaa aactcagtga gcagggcttg
    13141 atcacccgtc atcatggagg ggccgggcgc gtctccagcg tcatgaatac cgcttttgag
    13201 caacgggaac tttcgctcac cgccgagaaa cgggcgatcg ctgaggcagt cgccgattac
    13261 cttcccgaac gctgtaccgt ctttatcacc atcggtacaa ccgtagaagc cgttgccagg
    13321 gcattactca accggcgtga tttacgcatt atcactaata gcctgcgtgt ggcacagatt
    13381 ctttataaga atcaggatat tgaagtgatg gtgccgggag gcactttacg cgctcataac
    13441 ggtgggatta tcggcccagg agccgtggat tttattgaag gcttccgcgc agattattta
    13501 atcaccagta taggtgccat tgaacatgac ggcaccctac tggaatttga tgtcaatgaa
    13561 gcgttagtcg cgagaacgat gattaaacat gcgcggaata cattgttagt ggccgatcat
    13621 acgaagttta ccgcgtctgc tgccgtttca attggcaatg cacggaatgt cagggctttt
    13681 tttactgatg ccccgcctcc caattctttc tgccagttgt taagtgaaga gaatgttgaa
    13741 ctggtggttg ccgagcaaga agtatcctga aattgaaact tcggttgata cggttttcgt
    13801 tggccggatt aagcacatcc ggccactaat taagcttcag ccgccagcat ctctttctta
    13861 cgttggctaa tacgcttaac cagcattacg gaaaccaccg caccacagaa tgcgaagcag
    13921 gccataatgc caaacacgat tttgtagcca ataatccccg gatttaaatc cagaatgtag
    13981 ccatacagac tgaagcagaa catcgccggg gcgtaaccaa taaagctacc caacgccatc
    14041 gccgcgcctg ttttattttc agcaattttt gcttcgccga taggtgcaaa aaatacggct
    14101 cgctgtgtga agactatagc gccaaagccc agcgtacatg ccatccctaa atagaccggc
    14161 atactttcgt gcggcagcat aatcaacagt acgagcgcag cggtacttat gataaaggtg
    14221 tagcatagat atttactcgg cgatttcagg atcttatctg aaatcatgcc accaatcggt
    14281 cctccaatca ttttcagaca gtattggtta atgatgccgt aagcccccac cagagcaacg
    14341 ggcaatagat agatgttttt caggaatgga atgaagaatg ttaggccaca gtaaaccgcg
    14401 taaacgaaga agacgttgaa agcgataagc cagatggttt tatctctcag caccgaggtc
    14461 atactggtgt tttgcgatgc tccgtcttct tttttcacct caacggacgg tgcctcttct
    14521 ttgtcattca ggacaaagaa aatgataatg cctacggcaa tcacaataag ggaatagaac
    14581 cagatgcctg ctttaaaacc caataagcca ctgccaaacc aggtaaatac tgccaacgca
    14641 gaaaatgcca ccacggtatc gacaatgcca cgccctgttt cgaagaagcc aaacaaccga
    14701 ccttgttgtt cgctattgcc caatcgactt accgacttga gcaagaccgg ccagttcatc
    14761 atgtcgcaag taacgccaaa tagcgcccag acaaagagga tgccccaata ccccggcatt
    14821 gtcgtcaaat aaacacccag taatcctgtc gcaatgagtg agaatgacat ggtgtatctg
    14881 cgcggtagtt tatcggcaaa ataaatagac agaaaaaagc ccactgtggt gacaaatgag
    14941 tttaccgaca tagcattacc aatttgacca ttggtcaaat ggaaatattc ctgcatcggg
    15001 atataaaacg catctttcag agacggtaat ttataaattg taccgccaca caatgttaat
    15061 agactaaata acgcccattt ctttttcgtg agcataacat cccctaagcg atttttaaaa
    15121 tcactgtttt aaaggcaata agataccgac cattcatatg gaatggtttt attaacagcg
    15181 tatgagttaa aacttattag ttgttcagaa gatggtttgc ctcttgtttg atttgttgta
    15241 gtacattacg cacatgattt atctgattaa ttgcatcttc tttttcttta cgtaaccgcg
    15301 catccagtaa atgattttct ggcaatggtg tttcactcat ctggcgataa gggatccacg
    15361 gattatcatc ttcgtcttca aagcggaacg ccggcgcata gtaatcaacc tcttcttcca
    15421 ggccatatgc cgtcacctgc ggctcatcat cacccaggca gataaggtgc gttaataacg
    15481 ctttgaacgg catatcaccc tgacctgaaa tacaggcttt atgacccagg ccaccctctt
    15541 ctttaacgat caaggcatct ttgatatgga cctgggtaat atgcggtgcc atcgttttta
    15601 aagcgtcgat gggatgctca tttgcattga tcatgttcgc aaaatcaaac agtaaggaaa
    15661 gagattccat ctcactttct ttgaccagcg acaccagctc atggcttttt aaatcttcat
    15721 gctgttcgat cgtaaaagtc aggccgctgt cctgatacgt ttcccgtaca taggcaatgt
    15781 cgttagcgat gatcgataat acgtcgcgca aattaccttc ataacgtgga taaaaacgta
    15841 cggacgatgc cccagttttc aacgcaatgg cgacggcttc gtcaatagat gccttatctg
    15901 aagcgctggt ttcaatatga atatcaaggt tcagacggcg ggctttatcc ccaaaggcag
    15961 agagttcttt atcgtccata ttaccaagag aaaaacgctc gccatccaga acatggattt
    16021 tgacgccacg tagattattt tcacttgcga tatcaagtaa atcagcaggc aacactcttt
    16081 ccagccgcat atttaaatga aaggcataag catgtaaata aagaggaaga gtgtcgacac
    16141 gttgtagaat ctttcttgcg ttatttattg tcaccataaa acacctacag attaatgatg
    16201 aggtctacgg cctcaaatcg ccaatattca atatctatct ggacaatatt gccttgctgg
    16261 tcggcgcgat gcttttcaat taagatcgct ggcatacccg cagaaccgcc aagatacttg
    16321 ctgatatcgc ccggcattct gacgggtttg aaaatcaatt tttttactac cgtctctttt
    16381 tggtagcact tttcccagac tgcagaaaat gagtggtttt caagttgttc aataaaaccc
    16441 ggggcaactt caggattaat atatgtttcg tgatagaaaa ctttatgtcc ttccagcgct
    16501 ccccagccgg tgatgcgata gagttcagtt gatggtgtca ctgtaatcag tggcacgagc
    16561 gtctcgggaa tatctctggt acggcttttc tcggtaaacc cccaggaagg ctcccgccct
    16621 tgctcaattg cagcccgctg aaagctcgcc gacagctctg gactgtaatt aaatcgcggc
    16681 tgggtcacga accacccttt acgatcctta cggaagattt tggattctgc ctcgaggttc
    16741 aacaacgcct ggcgcagcgt catacgttta atgcccagca gttctcccag ctctctttct
    16801 gaagggagtt ttccacccgg cgtggtgatg ccatctttca accaccgatc cagtttctct
    16861 tttgcatttt ctacggtgga ttgattctcc gccattgttt aatccttttg gtttaaacca
    16921 gatgaggcat cttacgctta cgccaaaaaa gaaagcaatt aacggcgtat gactttgcag
    16981 ataattgtga caaagctctc tattgacgta atcacagact tataagggag atactcgcat
    17041 tggtgtgcgc tcacgacagg taaaaaaaaa cctgccagcg atggcaggtt ttttatgact
    17101 gaaaagcgaa attaatcgtc tttcggtgag cggttggcgc gacgacgatc gttttccgtc
    17161 aggtgacgtt tacgaatacg gatagaggtc ggagtcactt ctaccagttc gtcatcatcg
    17221 atgaactcca gagcttgttc cagagtcatg cggataggcg gaaccagaac aacggcttcg
    17281 tcagtaccgg aagcacgcat gttggtcagt ttcttaccgg tcaggcagtt tacagtcagg
    17341 tcgttagagc ggctatgaat accgataatc tgaccttcgt aaacttctgc accgtgaccg
    17401 aggaacagct taccgcgatc ctgcagaccg aacagcgcga acgcgaccgc tttaccctga
    17461 ccgttagaga tcagtacacc gttctgacgc tgacccactt cgcccggacg tacgtcgtcg
    17521 tagtggctga aggtggagta cagcagacca gtaccggaag tcatggtcat gaactcagaa
    17581 cggaagccaa tcagaccacg gcttgggatc acgtagtcga gacgtacgcg gcctttaccg
    17641 tctggattca tgtttttcag gtcgccttta cgttcgccca gcgcctgcat cacagaaccc
    17701 tgatgctgtt cttcaacgtc cagcgtcacg ttttcatacg gctcttgttt acgaccgtcg
    17761 atttcacgga agattacttt cggacgggat accgccagtt cgaaaccttc acgacgcatg
    17821 ttttcgataa gaacagacag gtgcagttcg ccacgaccag aaacgcggaa cgcatcggcg
    17881 tcttcggttt cttctacgcg cagcgcaacg ttgtgtacca gttctttgtt cagacgatcc
    17941 aggatctgac gagacgttac gaacttacct tctttaccgc agaacggcga ggtgttaacg
    18001 cagaagaaca tagaaacggt cggctcatca acggagagtg ccggcagcgc ttcaacgttt
    18061 tgcgtgtcgc aaacggtgtc agaaatgttc agttcgccca ggcccgtgat cgccacgata
    18121 tcgccagctt ccgccagatc ggtttcgata cgttccagac cgaggtggcc cagcacttta
    18181 ccgactttcg cgttgcgggt tttgccttcg ctatcgatga tagtaacctg ctggttcggc
    18241 ttcactttac cgcgcttgat gcggccaatg ccgataacgc caacatagct gttgtaatcg
    18301 agctgagaaa tctgcatctg gaacggaccg tcaaggtcaa cgtccggcgc aggaacgtgg
    18361 tcaacaatcg cctgatacag cggggtcatg tcttccgcca tatcttcgtg gtccagaccc
    18421 gcgataccgt tcagcgcaga agcgtaaacg atcgggaagt ccagctgctc gtcggtcgcg
    18481 tcgaggttaa cgaacagatc gaatacctga tccacaaccc aatcaggacg cgcgccaggg
    18541 cggtcaactt tgttgataac aacaatcggc ttcaggccgt aagcaaacgc ttttttggtt
    18601 acgaagcgcg tttgcggcat cgggccgtca aatgcgtcaa ccaccagcag cactgagtct
    18661 accatggaca ttacacgttc aacttcacca ccgaagtcgg cgtgccccgg ggtatcaacg
    18721 atgttgatac ggtaatcatt ccatttgata gcggtgtttt tcgcgaggat ggtaatccca
    18781 cgctctttct ccaaatcgtt ggagtccatc acgcgctctt gggtttcggc acgagagtcg
    18841 aacgtaccgg attgttggag cagcttgtct accagggtgg ttttaccatg gtctacgtgc
    18901 gcgatgatgg cgatattacg caatttttcg atcacaactt tgcctcaggc attagaaata
    18961 gcgcgttatt gtacacggat taatcgcact acaaaacagg atcacaaaca tcctccgcaa
    19021 acaagtattg cagagtccct ttgtgatcgc tttcacggag cataaaaagg gttatccaaa
    19081 ggtcattgca ccaatatggt gcttaatgtt tccattgaag cactatattg gtgcaacatt
    19141 cacatcatgg tgcagccctt ttgcacgatg ttaagcatga taacgccttt taggggcaat
    19201 ttaaaagttg gcacagtttt cgctttatct ttttacggcg acacggccaa aataattgca
    19261 gatttcgtta ccacgacgac catgaccaat ccaggagagt taaagtatgt ccgctgaaca
    19321 cgtactgacg atgctgaacg agcacgaagt gaagtttgtt gatttgcgct tcaccgatac
    19381 caaaggtaaa gaacagcacg tcactatccc tgctcatcag gtgaatgctg aattcttcga
    19441 agaaggcaaa atgtttgacg gctcctcgat tggcggctgg aaaggcatta acgaatccga
    19501 catggtgctg atgccagacg catccaccgc agtgattgac ccgttcttcg ccgattctac
    19561 cctgattatc cgttgcgaca tccttgaacc tggcaccctg caaggctatg accgtgaccc
    19621 gcgctctatt gcgaagcgcg ccgaagatta cctgcgttcc actggcattg ccgacaccgt
    19681 actgttcggg ccagaacctg aattcttcct gttcgatgac atccgtttcg gatcatctat
    19741 ctccggttct cacgttgcta tcgacgatat cgaaggcgca tggaactcct ccacccaata
    19801 cgaaggtggt aacaaaggtc accgtccggc agtgaaaggc ggttacttcc cggttccgcc
    19861 ggtagactct gctcaggata ttcgttctga aatgtgtctg gtgatggaac agatgggtct
    19921 ggtggttgaa gcccatcacc acgaagtagc gactgctggt cagaacgaag tggctacccg
    19981 cttcaatacc atgaccaaaa aagctgacga aattcagatc tacaaatatg ttgtgcacaa
    20041 cgtagcgcac cgcttcggta aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa
    20101 cggctccggt atgcactgcc atatgtcgct ggctaaaaat ggcgttaacc tgttcgcggg
    20161 cgacaaatat gcaggtctgt ctgaacaggc gctgtactac attggcggcg tcatcaaaca
    20221 cgcgaaagcg attaacgccc tggcaaaccc gaccaccaac tcttacaagc gtctggtccc
    20281 gggctacgaa gcaccggtaa tgctggctta ctctgcgcgt aaccgttctg cgtctatccg
    20341 tattccagtg gtttcttctc cgaaagcacg tcgtatcgaa gtacgtttcc cggacccggc
    20401 ggctaacccg tacctgtgct ttgctgccct gctgatggcc ggtcttgacg gtatcaagaa
    20461 caagatccat ccgggcgaag cgatggacaa aaacctgtat gacctgccgc cggaagaagc
    20521 gaaagagatc ccacaggttg ctggttctct ggaagaagca ctgaacgaac tggatctgga
    20581 ccgtgagttc ctgaaagccg gtggcgtgtt cactgatgaa gcgatcgatg cgtacatcgc
    20641 tctgcgtcgc gaagaagatg accgcgtgcg tatgactccg cacccggtag agtttgagct
    20701 gtactacagc gtctaatagt tgaagttgta ctacccggcg caacaacgcc gggatttagt
    20761 tgccgtggaa actttcagcc catctctgca tgggcttttt tctccgtcaa ttctctgatg
    20821 cttcgcgctt tttatccgta aaaagctata atgcactaaa atggtgcaac ctgttcagga
    20881 gactgcttta tggcaacagg cacgcagccc gatgctgggc agatcctcaa ctcgctgatt
    20941 aacagtattt tgttaatcga tgacaacctg gcgatccatt acgccaaccc cgccgcgcaa
    21001 caactgctcg cccaaagctc ccgcaaattg tttggtacac cgttaccgga actgttgagc
    21061 tacttctcat taaatatcga gctgatgcaa gaaagtctgg aggcggggca aggttttact
    21121 gataacgaag tgacgctggt catcgacggg cgctcgcata tcctttctgt gacggcccag
    21181 cgtatgccgg acggcatgat cctgctggag atggcaccga tggataacca gcgccgctta
    21241 agtcaggaac agctacagca cgcccagcag gttgctgccc gtgatttagt gcgcggcctg
    21301 gcacatgaga ttaaaaatcc gcttggcggt ttacgtggcg cggcgcagct gctcagcaaa
    21361 gcattacctg acccgtcact actcgaatat accaaagtga ttatcgaaca ggcggaccgg
    21421 ctgcgaaatc tggtcgaccg tctgttggga ccgcaactgc ccggtacgcg cgttaccgaa
    21481 agtattcata aagtagctga acgcgtggtg acgctggtgt cgatggaact gccggacaac
    21541 gtgcggttga ttcgggatta cgaccccagc ctgccggaac tggcgcacga cccggatcaa
    21601 attgaacagg tcttgctgaa tattgtgcgc aatgcgctac aggcgctggg gccggagggt
    21661 ggtgaaatca ttctgcgtac ccgcacagcg tttcaactga ccttacacgg cgagcgctat
    21721 cggctggcgg cgcgaattga tgtggaagat aacgggccag gtattccgcc tcatttgcag
    21781 gatacgctgt tttacccgat ggtcagcggc cgcgaaggtg gcaccgggct tggcttatcc
    21841 atcgcccgta atttgattga tcagcattca ggcaaaattg aatttaccag ttggccaggt
    21901 cataccgagt tctcggttta cctgcctatc aggaaataaa ggtgacgttt atgcaacgag
    21961 ggatagtctg ggtagtcgat gacgatagtt ccatccgttg ggtgcttgaa cgtgcgctcg
    22021 ctggagcggg tttaacctgt acaacatttg agaacggcgc ggaggtactg gaggcgctgg
    22081 cgagcaaaac gccggatgtg ctgctttcag atatccgtat gccgggaatg gacgggctgg
    22141 cgctgctcaa gcagattaaa cagcgccatc cgatgcttcc ggtcatcatt atgaccgcac
    22201 attccgatct ggatgctgcc gtcagcgcct atcaacaagg ggcatttgat tatctgccca
    22261 aaccgtttga tatcgacgaa gccgtggcgc tggttgagcg cgccatcagt cattaccagg
    22321 aacagcagca gccgcgtaat gttcagctta acggcccaac gaccgatatc atcggtgaag
    22381 cgccagccat gcaggacgtg ttccggatta tcggtcggct ttcgcgttct tctattagcg
    22441 tgctgattaa cggcgagtcc ggcaccggta aagaactggt cgctcatgcc ctgcatcgcc
    22501 acagtccgcg agccaaagcg ccgtttatcg cgctcaatat ggcggctatc ccgaaggatt
    22561 tgatcgaatc cgaactgttt ggtcacgaga aaggcgcatt taccggcgcg aataccattc
    22621 gtcaggggcg ttttgaacag gctgatggtg gcacattatt cctcgatgaa attggcgata
    22681 tgccgctgga tgtgcagacg cgtttgctgc gcgtactggc agatggtcag ttttatcgcg
    22741 ttggcggcta tgcgccggtg aaagtagatg tgcggattat cgctgccact caccagaatc
    22801 ttgaacagcg ggtgcaggaa ggtaagttcc gtgaggatct gttccaccgc ctgaacgtca
    22861 tccgcgttca tctgccgcca ctgcgtgagc gtcgggaaga tattccccgt ctggcacgcc
    22921 attttttaca ggttgccgcg cgcgaactgg gcgtagaagc gaagttgctg catccggaaa
    22981 ccgaagccgc gctgacgcgc ctggcgtggc cggggaacgt gcgccagctg gaaaacacct
    23041 gtcgctggct aacggtgatg gccgccgggc aggaagtgtt gattcaggat ttgcccggtg
    23101 aactgtttga atcaacggtt gcggagagta cttctcaaat gcaaccggac agttgggcga
    23161 cacttttagc gcagtgggca gacagagcgc tgcgttccgg tcatcaaaat ctgctttccg
    23221 aagcgcagcc tgagctggag cggacgttac tgacgaccgc gttgcgacat acgcaagggc
    23281 ataaacagga agcggcgcgg ctactcggct gggggcgtaa cactctgacg cggaagttaa
    23341 aagagctggg gatggagtga ttcacggctt gtgtgtaaag attgattatt gagcgcaaat
    23401 tgctggtgtt ttgcgcttta ctgttccgat aagttcagta tgatcttgcc cggaaaacgg
    23461 ggagagtcat tatgctggaa tcaataatta atctggtatc gagtggcgca gttgacagcc
    23521 acacaccgca aactgctgtt gctgccgtgc tgtgtgccgc gatgattggg ctgtttagtt
    23581 gagtttcaga ttgctgatga agtgctcttt gttcatgccg gatgcggcgt gaacgcctta
    23641 tccggcctac aaaagcaata ttttcaacag attttgcagg cctgataagc gtagcgcatc
    23701 aggcaatctt gttctcagca cattgatggc gctttcgttt tctactttgt aaacgaagcg
    23761 ccattcacaa tttaaatcac ccgagagaac tgctgcatcc gcgctttctg gcgcagatag
    23821 gtatcaaagc acatgcaaat gttgcggatc agcaagcgac ctttcgccgt cacctgtatt
    23881 cccttctcat ccacatccac cagcccatct tttgctaacg gggcgagcag cttgagatct
    23941 tcggcaaagt aatcagcgaa gagcaaatcc cactgttgct caatgggagc gtaatccaga
    24001 cggaagttgc agatgagcga cttaatcaca tcgcggcgaa tacagtcatc acgcgttagc
    24061 gcaataccac gccacagcgc attgccttgt tcatccactt gctgatagta ctgcttcaac
    24121 tctttctggt tctgcgcgta gcagtcgcca atcatgctga tggcggaaac gcccatcccc
    24181 agcagatcgg tatcgccctg agtggtgtag ccctggaagt tacgatgcag cacgccttca
    24241 cgctgggcca ccgccagctc gtcatccgga cgggcaaagt gatccatacc gataaactga
    24301 tagcccgatt gtgtcaggaa ggcgatggtt tcctgcagga tatcgagttt ttgctgcgga
    24361 ctcggcaggt cagcatcttt gattttgcgc tgagcagcaa aaatggtcgg cagatgcgcg
    24421 tagttaaaga cactcagacg atcggggttc agctccgcca cacgtttcag ggtaaaggcg
    24481 aaactctccg gcgtctgttt cggcaggccg taaatcaggt cgatgttggt ggaggtaaag
    24541 ccaatctcac gcgcatggtt aagcagtgca aagataaact cttcatcctg ctcgcggtta
    24601 accagacgtt gcacttcttt gttgaagtcc tgcacgccca tgctcaggcg attaaagcct
    24661 tcggcgcgta aatgatcgag tacatccagt tcgatttccc gcggatcgac ttcgatcgag
    24721 atctccgcat cggcattgaa ctggaagttt tcgcgcagca gcttcatcag acggctgatt
    24781 tgcgctttat tcagatacgt cggcgttccg ccgccccagt gcaattggct gacgtgacgc
    24841 ccggcgaaca gcggtgcacg atggacgatt tcttgctcca gcgcgtccag atactgatcg
    24901 gccttgtgct gctggcgagt aactatctta ttgcaaccgc agaagtaaca aagcttatgg
    24961 cagaacggaa tatgtacgta gagagataat ggacgctcag gatagcgcgc cacggcttgt
    25021 aaaaacgcct gttcgccgaa gtcttctgaa aactccagcg cggtcgggta cgaggtgtat
    25081 cgtggcccgg aatagttata tttctggatc agggccagat cccagtcgat ttgctgtaca
    25141 gacatgctca ctccttccga tggcgtctct ggcggctacg gcgtaccggc ggatgcccgt
    25201 ttcgagtcat cagccggttg cgcagccact tttgtcgccg cgacaaccgc tgtagtttaa
    25261 cgaataacca caccagataa catataaccg gaagggttat cagcagaacg ggaaggccca
    25321 cggcggcaat ccgttagttg ccccgcagca gacgcatcat gtcttcttgc ttctcgtctt
    25381 cttcctcttc ttcgtcatcg tcataagaga ggccgagttt ctgcatcaac tcatcaatac
    25441 gatccagttt ggcatccacc caggattgct cttcggcgct cagggtttcg cctgcttcca
    25501 gacgttccag cagcgcatcc agacgctcat ccgtttccag taactccaac tccgcctgcg
    25561 gtgaaagcat aggtttctca ctcttcggtt tgtgctgttt ggtgactttt tcagtcacgc
    25621 ccaatggaat aggggtttta ctgccaatac gtggatcttt cggtgcgttc tggcctttgc
    25681 tgcctgacgt ggtgttaccg cccgctgcgc ggctgcccgg cgcatgacca cgacgttttt
    25741 tctgacgctt acggtcacga gcttcctgat ccagctcctc gcgtgttttt cgacgcgctt
    25801 ttgcatgacc tttgctgcgt gagtttgaag atgatggttt cataatggtt cgtcttttag
    25861 ccgttttatt cagtatagat ttgcggcgga atctaacaga aagcaagcaa agaaaaaagg
    25921 cgacagatta ctctgtcgcc ttttttcctg actcataacc cttaacgggt cttacaatcc
    25981 ttgcaggccg ccaacacacc ctgtttttcc attagccgga acaatccgtg ttgattccat
    26041 ttccgtttaa ttacgtctcc ggacgtttat cttcctgaca atcctctgtc ttcgcctcct
    26101 ggcgctcctg accctcatcc tgagtcattg ttcctgttag cgtcctcgct ctatgggtgc
    26161 tactttacgc ctttgcttta aataaacaat caaccgccat cagtatttgc acattttcag
    26221 agttttatat aaaagcaatt cactgattta tatagcttat ttgacatacg tcgcagaatt
    26281 ttctctgcta tttcgcataa agactatcgg caatgtctca caaagcatat ggcttttact
    26341 tacaacgctc acgccgaaaa atccgccttt tattatggtt caggtataat cgccgcaaag
    26401 ctttgactga tggagacgac cgctttgact aatttgaatt atcaacagac gcattttgtg
    26461 atgagtgcgc ctgatattcg ccacctacct tccgataccg gaattgaagt ggcttttgca
    26521 ggccgttcca acgcaggtaa atccagcgcg ctgaacacgc tgactaacca gaaaagcctg
    26581 gctcgtacct caaaaacccc ggggcgcacc cagcttatca acctgtttga agtggctgac
    26641 ggcaagcgtc tggttgactt gcctgggtac ggttatgcgg aagtcccgga agagatgaag
    26701 cgcaaatggc agcgcgcgct cggcgagtac ctcgaaaaac gtcagagcct gcaaggtctg
    26761 gtggtgctaa tggatattcg ccatccgctg aaagatttgg atcagcagat gattgagtgg
    26821 gcggtagaca gcaatatcgc cgtgctggta ctgctgacca aagcggacaa actggcaagc
    26881 ggcgcacgta aagcgcaatt gaatatggtg cgtgaagcgg tgctggcgtt taacggtgat
    26941 gtgcaggttg aaacgttttc ttcgctgaag aaacaaggcg tggacaagct gcggcagaaa
    27001 ctggatacct ggtttagcga gatgcagcct gtagaagaaa cgcaggacgg cgaataattt
    27061 tcttgcctta atgcttgtgc cggatgtggc gtatcaggca ttacggatct tttctttcgc
    27121 ccaataaaaa acgccccagt cattactgac tggggcggct aaaatattca gccaaatccg
    27181 attacgtgaa gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc tacgcgacta
    27241 actttactct tttttgttca gttcagaaag cattttttgt aatttttttt cagttgttgc
    27301 ataggaattt ctaatagcca tcacaaaacg tgacagctta tgttgcttac ttacgaaaaa
    27361 agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt tttcgccact ccccacttcc
    27421 accagcagcg gcacatccag acgggtacag ttttccatca gttgatgaat ctgcttcgcg
    27481 acggcatcga catcatcttt atgaacttca aataccagtt catcgtgtac ctgcatgatc
    27541 atacgcacac gcggttgctc agcctgtagc cacgcatcaa cggcaatcat cgcccgtttg
    27601 ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc agccgctgca
    27661 cgacgagccc cattgctgga tttgatatcc ggcagataca gacggcgtcc gtccagcgtt
    27721 tcaacgtagc cctgctcttt cgcctgagca cgggtgcgtt ccatatactc cagcacgcca
    27781 gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg aatgttcaat
    27841 tgccgcgcca gaccgaaagc actcatgcca taaatcagac caaagttgat cgctttcgcg
    27901 ctacggcgtt gctcgctggt tacggtttcc agtggcaaac caaacacttc tgccgccgta
    27961 gcccggtgga tatcttttcc ttccgcgaat gcggtcagca agcctttgtc acgcgagaga
    28021 tgcgccataa tgcgcagttc aatctgcgag tagtccgccg agacaatcac ataatcctct
    28081 ggcgcaataa acgcctggcg gatacgacga ccttcttcgt tacgcaccgg aatgttttgc
    28141 aggttaggat cggttgacga taaacgtccc gttgcagtta ctgcctggtg ataagaggta
    28201 tgcacacgcc cggttttcgg gttgatcatc agcggcagct tgtcggtgta agtcgatttc
    28261 agcttcgcca gaccacgata ctccagaatc acttttggca acggatagtc cagcgccagt
    28321 tcttccagta cctcttccga cgttgacggc gcgccacccg gcgttttctt cagcggttta
    28381 attccctgtt tttcaaacag aatggtttgc aactgcttgg tggaggaaag gttaaattct
    28441 tcacctgcaa tttcatgcgc tttcttttcc agctcagcca gacgaagagt gagctcttca
    28501 gaatgattgt gcagcacttt cggatcgatc ttcacaccgt tacgttcaat gcgtgaaagc
    28561 accggcacta gcggcatttc gatattctcg aagacgttca acggcccttt gtgtttttgc
    28621 agatccggcc acattttcag atgcaactgc aaggtgacat ctgcatcttc ggcggcgtaa
    28681 cgtccggcct cttcgagggc aatctggtta aaggtcagtt gatttttgcc tttacccgca
    28741 atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct gtccatatcg
    28801 tgacgcccgg caacgctatt gagaatgtag gactccagca tggtatcaaa cgcaatccca
    28861 cgcagctcaa tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt ttgcccaacc
    28921 ttcagcgcct tttcatcttc cagcagcggt tttagcaact cgagtgcacg ctcgcgagag
    28981 atttgatcgg gcgcatcaag ataatcatga gcaaccggaa tatatgccgc tacgcctggc
    29041 tcgatagcaa aagaaagccc gaccaggtta gcagagatgt tatcaaggct gtcggtttcg
    29101 gtatcaaatg caaataccgg cgctttttcc agcttcgcaa tccacgcttt cagtgtttct
    29161 tcatcaagga tggtgacgta gttgtcataa gaaatcaccg ttgccgtcac ttctggcgct
    29221 tcgtctacaa cactggtttc ctgcggcttc gcggctggtt ttgccccttt ggcctgtaac
    29281 catttgcccg cttcgacatc agcagtccag cgtttgaact catacttttt gaacagccct
    29341 aacaactctt ctgccaccgg tggctgcact tccagttgtt cacaggtcag ctccagttca
    29401 acgtcggttt taatcgtcgc cagctggtaa gagagataag ccacttcttt gttttgctcg
    29461 agcttcgccg ccattgtttt cgcgccacgg aagctcaacc cagcaatttt ttctggctcg
    29521 gcatacagcg tatccagtcc gccaagacct tgtagcaatg cttgcgcggt tttttcaccg
    29581 acgcccggta cgccagggat gttatcagag gagtcaccca tcagcgccag gaagtcgatg
    29641 atcagttctg gcggcacgcc gtacttattc accacctctt ccgggccgag gatagtattc
    29701 gtcatggtat tgataagcgt aatatttggc gtcaccagct gcgccatatc tttatcgcct
    29761 gtgcttatca gcaccggacg cccggctttt tcggcttccc gcgccagagt accgataacg
    29821 tcgtccgctt ctacgccaga aaccgccagc agcggcagtc ccatcgcttt aaccatcgcg
    29881 tgcaagggtt cgatttgtgc acgcagatcg tccggcattg gcgggcgatg tgatttgtaa
    29941 tgttcaaaca gttcatcacg aaaggttttt cccttggcgt caaagaccac cgctgcatgc
    30001 gtcggtttat attgcatgat tagactgcgc agcatgttga ggacaccata catcgcaccg
    30061 gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg ataaagataa
    30121 gatgaaccat ctacaaggat aagtggattt tgggggatct gaaccataat gtccgtgcct
    30181 gtttatcaga ttatgggtaa aggatgccac agacaggatg aaaacatgag tttttcaccg
    30241 cttttcgtga aaatcttttg tagatcttct ggatcgctcg caaatcgtca tgtggataac
    30301 tttgtgcata aattatttac atcgctttta atttattatc atgcttttta cagcatgata
    30361 tttgtctttg cttttcatac agttaaatat aaagccatcc ctaataattg tgataatggg
    30421 attttttcta atgtggatat atatgaatta tttttctcct taaggatcat ccgttatttg
    30481 gggcgttttc tgctaaaatc ggcgccttgc gcttctttag cgcactgtct atggctaacc
    30541 ttttgaataa attcattatg acgagaatac tcgctgcgat aacccttttg ctgagtatcg
    30601 tattgaccat tttggtcact attttttgct ccgtcccgat catcattgcc gggattgtaa
    30661 aactattgct acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt gattttatga
    30721 tgtattgctg gtgtgaaagt ctggcggtat tactgcatct gaacccacac ctgcaatggg
    30781 aagttcacgg gctggaaggg ttaagtaaga agaactggta tctgcttatt tgtaaccacc
    30841 gtagttgggc agatattgtc gtactgtgcg tgctgtttcg taagcacatt ccgatgaata
    30901 aatatttcct caagcagcag cttgcctggg tgccatttct tggcctggca tgctgggcgc
    30961 tggatatgcc gtttatgaag cgttattccc gcgcttattt attacgtcat ccggaacgac
    31021 gtggtaaaga tgttgaaacc acccggcgtt cttgtgaaaa gtttcgcctg caccccacca
    31081 ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag caaacgcact
    31141 ccacttttca aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg ctaaatgtac
    31201 tgggtaaaca attcgataaa ctgcttaatg ttacgctgtg ttatccggac aataaccgtc
    31261 agccgttctt cgatatgtta agcggtaaac tgacgcgaat tgtcgtacat gtggatttgc
    31321 agcctatttc cgatgagtta catggcgact acatcaatga caaaagcttt aagcgccatt
    31381 ttcagcaatg gctgaactca ctgtggcagg aaaaagaccg gcttctgacg tcgttgatgt
    31441 catcacagcg gcaggacaag taaattttgc tcgcctctga atgagcagag gcgagtgagt
    31501 attttaatga acaaaacgtc cggcacgaga cataaattct tcttcgctca tctcctgtcc
    31561 gttaaaaaca actttacccg gtgtataacg cagttgcagc gaggcggtat tgttgtcaat
    31621 cgtgattaac tgaaacatct gacctaatgt catcatcccg ctgatttgtt tatcagcccg
    31681 cttttgcgct ttttcagcat ccattccttc agataaatta agctgttttg cggtttctgt
    31741 gacaacatta agcggtaact tcacatcaaa aatgagcgat ttaatatctt tgtttgtgga
    31801 tgatgaggat ttggctgggt cggcaatact gatatccaga ttggcattta gttcgccgag
    31861 tgcattcttc catcttaccg gttgtttaat ggtcggctca cttttttgta ataacggtaa
    31921 atactctttg aacaatgcag cattcacttc ttgcagagca acttcatcgt ttgctaactc
    31981 agggtgtgca gcaagctgct tctgcatcgc aatgttatac tggatgacaa attggcgcac
    32041 cgctgaggga tcaatagatt cagcaatcaa tgaccactca ccactaccga gttgctgatt
    32101 cccttttgtt aacttatcaa gttcgtaggt cagattgaca ttgatataat ctttgtcttt
    32161 aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt cttctccctg
    32221 attgataatg ttaatctgat tgaatttcga ctcgctttcg ccaaccggaa atttcttttc
    32281 ttctaatcgc gttagagaat caatggtaaa acttttcgcc gttactttat tcaatggcga
    32341 taattccaga acaagatctt cgactttgcc ttcaatatcc agattagaca atgaactacc
    32401 atcaccatta aaagtaaact ggccctcacc aaaagaaaac tttccatatt ttgcaggggc
    32461 aacatctaat gtcgtggaga atgagccacc aaacgcgaat cgcaatgtcg cttccatagg
    32521 agatttattt tttgcggcga taaatagcgg ttgtgtcagt tcattatcca ctaatttcac
    32581 ttttgccgct gccagtgcgg ggatgacatt accacgcatt aacattgtga tgggtaacgg
    32641 accgtgctca acatccacat caaacgcaac tttctggcca ggtttgatat tgagatcagg
    32701 tgcaccgtta tcaaatgtca tttgcatttg aaaacgagaa ttaaaaaagc ctttgtcaaa
    32761 atttttataa cttaacgtca tatcgtaggc atgttcacct ttctttttag catcgttaaa
    32821 atctttaata aacttttcga taccaggctg aatttgcgta cctgtgtacc atgtgccacc
    32881 accccagatt acggctaacg caacaataac acctgtagct gacttacgta tcatataaat
    32941 attccctgat aaaacagcaa aactttgaat atccacttat gctgaaataa tgt
//
LOCUS       DAZKGF010000032        32871 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZKGF010000032 DAZKGF010000000
VERSION     DAZKGF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.032"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            155..1372
                     /gene="alaA"
                     /locus_tag="ACHOQV_001494"
     CDS             155..1372
                     /gene="alaA"
                     /locus_tag="ACHOQV_001494"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFP9629889.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1456..2055
                     /gene="yfbR"
                     /locus_tag="ACHOQV_001495"
     CDS             1456..2055
                     /gene="yfbR"
                     /locus_tag="ACHOQV_001495"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP9629890.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2114..3946)
                     /gene="yfbS"
                     /locus_tag="ACHOQV_001496"
     CDS             complement(2114..3946)
                     /gene="yfbS"
                     /locus_tag="ACHOQV_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP9629891.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4033..4683)
                     /gene="hxpA"
                     /locus_tag="ACHOQV_001497"
     CDS             complement(4033..4683)
                     /gene="hxpA"
                     /locus_tag="ACHOQV_001497"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFP9629892.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4694..5188)
                     /gene="yfbU"
                     /locus_tag="ACHOQV_001498"
     CDS             complement(4694..5188)
                     /gene="yfbU"
                     /locus_tag="ACHOQV_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP9629893.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5272..5727)
                     /gene="yfbV"
                     /locus_tag="ACHOQV_001499"
     CDS             complement(5272..5727)
                     /gene="yfbV"
                     /locus_tag="ACHOQV_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFP9629894.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6065..7267
                     /gene="ackA"
                     /locus_tag="ACHOQV_001500"
     CDS             6065..7267
                     /gene="ackA"
                     /locus_tag="ACHOQV_001500"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFP9629895.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7342..9486
                     /gene="pta"
                     /locus_tag="ACHOQV_001501"
     CDS             7342..9486
                     /gene="pta"
                     /locus_tag="ACHOQV_001501"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9629896.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9676..11196
                     /gene="yfcC"
                     /locus_tag="ACHOQV_001502"
     CDS             9676..11196
                     /gene="yfcC"
                     /locus_tag="ACHOQV_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP9629897.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11229..11771)
                     /gene="yfcD"
                     /locus_tag="ACHOQV_001503"
     CDS             complement(11229..11771)
                     /gene="yfcD"
                     /locus_tag="ACHOQV_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFP9629898.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11829..12380)
                     /gene="yfcE"
                     /locus_tag="ACHOQV_001504"
     CDS             complement(11829..12380)
                     /gene="yfcE"
                     /locus_tag="ACHOQV_001504"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFP9629899.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12436..13080)
                     /gene="yfcF"
                     /locus_tag="ACHOQV_001505"
     CDS             complement(12436..13080)
                     /gene="yfcF"
                     /locus_tag="ACHOQV_001505"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFP9629900.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERSTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13216..13863
                     /gene="yfcG"
                     /locus_tag="ACHOQV_001506"
     CDS             13216..13863
                     /gene="yfcG"
                     /locus_tag="ACHOQV_001506"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFP9629901.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     HISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAASQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13920..14282
                     /gene="folX"
                     /locus_tag="ACHOQV_001507"
     CDS             13920..14282
                     /gene="folX"
                     /locus_tag="ACHOQV_001507"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFP9629902.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14303..15196
                     /gene="yfcH"
                     /locus_tag="ACHOQV_001508"
     CDS             14303..15196
                     /gene="yfcH"
                     /locus_tag="ACHOQV_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFP9629903.1"
                     /translation="MKIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VALWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCAHWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15244..16146)
                     /gene="rpnB"
                     /locus_tag="ACHOQV_001509"
     CDS             complement(15244..16146)
                     /gene="rpnB"
                     /locus_tag="ACHOQV_001509"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFP9629904.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLE
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16343..17116)
                     /gene="hisP"
                     /locus_tag="ACHOQV_001510"
     CDS             complement(16343..17116)
                     /gene="hisP"
                     /locus_tag="ACHOQV_001510"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFP9629905.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17124..17840)
                     /gene="hisM"
                     /locus_tag="ACHOQV_001511"
     CDS             complement(17124..17840)
                     /gene="hisM"
                     /locus_tag="ACHOQV_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9629906.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17837..18523)
                     /gene="hisQ"
                     /locus_tag="ACHOQV_001512"
     CDS             complement(17837..18523)
                     /gene="hisQ"
                     /locus_tag="ACHOQV_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFP9629907.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18613..19395)
                     /gene="hisJ"
                     /locus_tag="ACHOQV_001513"
     CDS             complement(18613..19395)
                     /gene="hisJ"
                     /locus_tag="ACHOQV_001513"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFP9629908.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19616..20398)
                     /gene="argT"
                     /locus_tag="ACHOQV_001514"
     CDS             complement(19616..20398)
                     /gene="argT"
                     /locus_tag="ACHOQV_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFP9629909.1"
                     /translation="MKKSILALSLLVGLSAVASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVRDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20664..21233)
                     /gene="ubiX"
                     /locus_tag="ACHOQV_001515"
     CDS             complement(20664..21233)
                     /gene="ubiX"
                     /locus_tag="ACHOQV_001515"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFP9629910.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21328..22845)
                     /gene="purF"
                     /locus_tag="ACHOQV_001516"
     CDS             complement(21328..22845)
                     /gene="purF"
                     /locus_tag="ACHOQV_001516"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP9629911.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22882..23370)
                     /gene="cvpA"
                     /locus_tag="ACHOQV_001517"
     CDS             complement(22882..23370)
                     /gene="cvpA"
                     /locus_tag="ACHOQV_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFP9629912.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23629..24279)
                     /gene="dedD"
                     /locus_tag="ACHOQV_001518"
     CDS             complement(23629..24279)
                     /gene="dedD"
                     /locus_tag="ACHOQV_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFP9629913.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAVNNTEFEPEPA
                     PVAPPKPKPVESPKPKVEAPPASKPVVEEKAAPTGKAYVVQLGALKNADKVNEIVGKL
                     RGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYTPN"
     gene            complement(24269..25537)
                     /gene="folC"
                     /locus_tag="ACHOQV_001519"
     CDS             complement(24269..25537)
                     /gene="folC"
                     /locus_tag="ACHOQV_001519"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFP9629914.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYTSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKSA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25607..26521)
                     /gene="accD"
                     /locus_tag="ACHOQV_001520"
     CDS             complement(25607..26521)
                     /gene="accD"
                     /locus_tag="ACHOQV_001520"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP9629915.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26677..27336)
                     /gene="dedA"
                     /locus_tag="ACHOQV_001521"
     CDS             complement(26677..27336)
                     /gene="dedA"
                     /locus_tag="ACHOQV_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9629916.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAFN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27419..28231)
                     /gene="truA"
                     /locus_tag="ACHOQV_001522"
     CDS             complement(27419..28231)
                     /gene="truA"
                     /locus_tag="ACHOQV_001522"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9629917.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28231..29244)
                     /gene="usg"
                     /locus_tag="ACHOQV_001523"
     CDS             complement(28231..29244)
                     /gene="usg"
                     /locus_tag="ACHOQV_001523"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9629918.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29310..30467)
                     /gene="pdxB"
                     /locus_tag="ACHOQV_001524"
     CDS             complement(29310..30467)
                     /gene="pdxB"
                     /locus_tag="ACHOQV_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFP9629919.1"
                     /translation="MSRTEKLLTILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLAD
                     ADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDDTWLKQAGIGFSAAPGCNAIAV
                     VEYVFSSLLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRG
                     DEGDFRSLDELVQHADILTFHTPLFKDGPYKTRHLADEKLIRSLKPGAILINACRGAV
                     VDNTALLTCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQ
                     VFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLR
                     KVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            complement(30627..31631)
                     /locus_tag="ACHOQV_001525"
     CDS             complement(30627..31631)
                     /locus_tag="ACHOQV_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9629920.1"
                     /translation="MSIKKLEDGRYLLDIRPNGRKGKRVRKVFNKKSVAVATERYIMA
                     NAEKREYIQGYRDRRTLNDLLELWWVYHGQHRRKAEEDRKQLRNIINELGADMQAVDL
                     DKLKIIAWRSRRIADGLKPSSANRYMNRLSGMFTALKKIGLWDAEHPVRGISVLYVSP
                     REMAFLSQKEVALLLDTLEGDYWRVALLCLSTGARWGEASKLRGEQIVHNRVTFLETK
                     NGRKRTVPISQAVCEAIKTSETGGLFDVKYREFCLVLKRVKPDLPKGQAAHVLRHTFA
                     SHFVMNGGNIIALQRILGHATIQQTMAYAHFAPDYLQDAVALNPLKGGVSVHSVSTGE
                     "
     gene            complement(31728..32057)
                     /locus_tag="ACHOQV_001526"
     CDS             complement(31728..32057)
                     /locus_tag="ACHOQV_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9629921.1"
                     /translation="MPDMASLSSMGEKFRLIREAEGMTRQEFADCVGLPYGTVTNYEV
                     RGKQVTEGALLKVTKHPKFKKYAYWLSTDETMPEAGQIAPALSLDGSCNLEGNQDSTE
                     TTRKLPR"
     gene            32162..32449
                     /locus_tag="ACHOQV_001527"
     CDS             32162..32449
                     /locus_tag="ACHOQV_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFP9629922.1"
                     /translation="MRESELEGFIQVAPYPLEAVPYQLFAKMIGRKESTVRTMIDAAK
                     LPTIDFVKPGSVKTRASENWVYLPAFNAGMRKAFFNQPKEQRDAWLLWLGL"
     gene            32645..>32871
                     /locus_tag="ACHOQV_001528"
     CDS             32645..>32871
                     /locus_tag="ACHOQV_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP9629923.1"
                     /translation="MTQRRSFRTGTERHAARFSTSASRSNTHYSLQETHATPDGHLVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSL"
BASE COUNT         8205 a         8958 c         7943 g         7765 t
ORIGIN      
        1 tgccattatt aagttattaa cagcacaatc gagtcttccc ctctggcaaa atcttattct
       61 gcagaccttc aaaacgccgt cctgggggag tacattgttc taagctgact tccacggcag
      121 ggagtggcga taacagcaaa aaaggtcaag attcatgtcc cccattgaaa aatccagcaa
      181 attagagaat gtctgttacg acatccgtgg tccggtgctg aaagaagcaa aacgcctgga
      241 agaagaaggt aacaaggtac tgaaactgaa catcggcaac cctgccccgt tcggttttga
      301 cgcgccagat gaaatcctcg ttgacgtgat tcgcaacctg cctaccgctc aagggtattg
      361 cgattccaaa gggctttact ccgcgcgtaa agccatcatg cagcactacc aggctcgtgg
      421 catgcgtgat gttaccgtgg aagatattta catcggaaat ggtgtatcgg agcttatcgt
      481 tcaggcaatg caggcattgc tgaacagcgg cgacgaaatg ttggttcctg cacctgatta
      541 cccactatgg accgcggcgg tttcgctctc cagcggtaaa gcggtgcatt atctttgcga
      601 tgaatcctct gactggttcc cggaccttga tgatattcgc gctaaaatta cgcctcgtac
      661 gcgtgggatc gttattatca acccaaataa cccaaccggc gcggtatatt ccaaagagct
      721 tttaatggag atagtggaga ttgcacgtca gcataatctc attatcttcg ccgatgaaat
      781 ttatgacaaa attctctacg acgacgctga gcatcactca attgcgccgc tggcacctga
      841 tctgctgacc attaccttta acggcctgtc gaaaacatac cgcgttgcag gcttccgtca
      901 ggggtggatg gtgttgaacg ggccgaaaaa acacgccaaa ggctacatcg aaggtctgga
      961 aatgctggct tcaatgcgcc tgtgtgctaa cgttcctgcg caacacgcca ttcagaccgc
     1021 gctgggcggt tatcagagca tcagtgaatt tattacccct ggcggtcgtc tttatgagca
     1081 gcgtaaccgc gcatgggaat tgatcaatga tattccgggc gtttcctgcg tgaaacctcg
     1141 tggtgcgctg tacatgttcc cgaaaatcga cgccaaacgc tttaatattc acgacgatca
     1201 gaaaatggtg ctggatttcc tgttgcagga aaaagttctg ttggtgcaag ggacggcatt
     1261 caactggccg tggccggatc acttccgcat tgtcacgcta ccgcgtgtcg atgatatcga
     1321 gctgtctttg agcaagttcg cgcgtttcct ttctggttat catcagctgt aatcttaatt
     1381 tcactgccgg agattgcatc cggcagtgtt atcccgccac aatgacctga tgatgtcatc
     1441 atacgtaagg tcactatgaa acagagccat ttttttgccc atctctcccg cctgaaactc
     1501 attaaccgct ggccgctaat gcgcaatgtg cggacggaaa atgtgtccga acacagtttg
     1561 caggtagcga tggtcgccca tgcgctggca gctatcaaaa atcgtaaatt tggcggtaat
     1621 gtcaacgccg aacgtatcgc tttactggcg atgtaccacg atgcctcaga agtgcttacc
     1681 ggcgatctcc ctaccccggt gaaatacttc aattcgcaaa tcgctcagga atacaaggcg
     1741 attgaaaaaa tcgctcagca aaaactggtc gatatggttc cggaagagtt gcgggatatc
     1801 tttgcgccgt taattgacga gcatgcatat agtgatgaag aaaaatcact ggtgaaacag
     1861 gcagatgcac tatgtgcata tctgaaatgt ctggaagaac tcgcggcggg aaataatgaa
     1921 ttcttgctgg caaaaacgcg actggaagcg acgcttgaag cgcgccgcag ccaggagatg
     1981 gactacttca tggaagtctt tgttcccagc ttccatcttt cgctcgatga gattagccag
     2041 gattcaccgc tgtaagcagc cggagtctgc gtcgcatcag gcaataagcg ccggatgcga
     2101 catcaggctc ttgtcaaaac ggaaacagca tcgggatcat caccacacaa accgccatca
     2161 cgataatggt gaacggtacc cccaacttca caaagtcact gaagctgtaa tttcccggac
     2221 ctaaaaccag tgtgttaaca ggtgaagaaa ccggcgtcat aaaggcggcg gatgctgcca
     2281 tcgccacgac catcgcgaat gggtaaggcg acacccccat cgttttagca gcagccagcg
     2341 caatcggagc catcaacacc gccgtcgcgg tattagaaat aaatagccca ataaccgccg
     2401 acaagacaaa caaacaaccc agcatcatat gtggcccgta accgccgcca atgtccatca
     2461 gccctttcac cgccagcgca acacctcccg ttttctgtaa tgccacagca aacggcatca
     2521 tcccaacgat caaaataatg ctcggccagt gtatggattt ataggcgctt tcagcatcga
     2581 tacagcggaa tttccccatc agcaggcagg cgatgatagc ggcaacagga ttaggaattt
     2641 catcggtcag cattaacgcc accattagca ccagacagaa aatggcatgg ggtgcctgac
     2701 tgtgcgcggg cgatgcttca ctcacctctt ccggtaagtt cagcgctacg aagtcgcggc
     2761 cctgtttggc cagcataccg atcagtttcc agttacccac aaccaggatg atatcgccca
     2821 gcagcagagg ctcatccgcc agcgaacctt ccagcgccac gccattgcgc tttagcccca
     2881 ctacattcaa tccgtagcgg gtacgaaaac caatttcgcg caccgattta ccaataagtt
     2941 ctgactcagg aattaatgaa atctctgcca tgcccacatc aagggcctgg tcagaaaaat
     3001 actcgccgcg cagtaccatc ggctccagca attgctcact acaaaattgc cggagatcga
     3061 catcagccgc agacatatca ataagcaaaa cgtcacgcgc gcgaaattca gaaaccccat
     3121 taacgttcac gataacgcga cgaaaacgcc gccagcgttc aacaccgatg acgttagcac
     3181 cataacgttc acgtaatttg agatcgtcca gtcgttgacc aatcattggc gatccggggc
     3241 gaatagccag acgtcgcgca cgcccggtca gtcgatattc acggataaga tcgcgaaagg
     3301 ttcggcgcgt ccagccttcg cgttgtgggg tctgggtatc ccctttcagc atgaaacgca
     3361 tcactaacat atacaaaata cccagcacca gcacgaccag gccaataggt gttacgctaa
     3421 agaaactgaa gccgtgatag ccttcacgca gcaattcact gttgactacc aggttcggcg
     3481 gcgtcgccac cagcgtcatc atgccgctta tcagcccggc aaaacttaac ggcatcatca
     3541 gacgcgacgg cgacgtttgc atacgcatgg caacgcttaa caccacggga ataaagatag
     3601 cgacaacgcc ggttgaactc ataaacgcgc caagccccgc gacggtcagc atcaacaaaa
     3661 ccaacatttt gatttcactg ttgcccgcca ctttgaccag ccatgtcccc attacagtgg
     3721 caacaccggt acggaccaaa ccatcgccaa taatgaacaa ggcggcaatc aggacaacgt
     3781 taggatcgga aaagccggaa aatacttcgg ggaccgtcag cgttccgctt aatgcaaacg
     3841 cgacaataac aaacaaagcg accgcatcca tacgcactct gcccgtcgca aacaagacga
     3901 tggcaacggc cagtaatgaa agaacccaaa tcaattcacc gttcacaact tatccttgtt
     3961 aattgagggg gataacttga ttctgccata aaaaagcccc gacgagacgg ggctaaatca
     4021 tgatcaggtg tttcactgaa taataacatc gccatttggc tgtttggtca cagtaatttg
     4081 ctccagactg tggaggacca aatcgacctc attcaggcgc ggggtatctg ccggagcgtt
     4141 aaccgcaatg acatggcaac ccgccgccag gccagaaagc acgccagcgg gagcatcttc
     4201 caccactaca cactcctgcg gcgcaagccc cagcagctgc gcgcctagca gatacgcatc
     4261 aggttctggt tttccgcgct tcactcgctc agcggtcaca aacacctctg gcgcgggaag
     4321 cccagctatt ttatggcgcg ctcgcgctac cggcatggag ccagaagtca caatggccca
     4381 cggaatacct gctttattca aatgattgag taaggcgatt gcccccggaa gtgcggtaat
     4441 accctcggtt tccgtggcct cgatttgctc cagacgcgta aactcggcgg caatatcagc
     4501 ctcggatttg cccgccataa aatggcgcaa agaggtgatc gcctgtttac cgtgaataaa
     4561 agccagcacc tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc
     4621 taccgcaggc agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg
     4681 cacgcgggcc tcctcaggcg ttgataattt gattaatttc gttggcgctc aaatggtact
     4741 gacgcgggca ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct
     4801 gggcgttaaa accgtgagtt ccggcatcaa aatgggtata gcgtccttcc acattaacca
     4861 taaagcggac ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga
     4921 cacgacgttc atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat
     4981 gatacatctc cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact
     5041 cgcgatccag ttcacgcatc tgtaatccat aaccacgctc aatgattgtt tgcaggcgac
     5101 ggtaacgttc agcatttgcc ggatcgagca tagtcatcat cttgtactgg ttagacaaaa
     5161 taagacgttg cgcgttggtc atttccattg ttgactcctg tatcactcta ctacggtgaa
     5221 aaaaaaagaa ggctgagtat gccttctttt atatgcgtaa tcaggggtca attacaaatc
     5281 atcaaggaaa gttttatcca gttgtttgaa ggcgcgctta agcgtgtcag ctaatgcctg
     5341 gtaatcaggc ttgccttcaa cgggtgccaa cacctgtcca gactcctgca atttaccgcg
     5401 aacttcataa aaccagttaa ggattgcagg gggtaatggc gttacagaac gtttgcccag
     5461 ccaccacaat ccctgcatgg gtaaacttaa ggcgaacagc gcagtggcaa ctgccggccc
     5521 aagctgaccg cccagggcaa tctgccagca gagagtaaat acggcgatcg gcggcataaa
     5581 acggatcgca taacgcgtca tcttgataac gcgattttcg acaaagaccg gggcaaggcg
     5641 tttttccagc ggccacgtct ttgagtaatg ctgtccccgg cgaaacaagc taaaaaaatt
     5701 aacagaacga ttatccggcg ttgacatgct tcacctcaac ttcacatata aagattcaaa
     5761 aatttgtgca aattcacaac tcagcgggac aacgttcaaa acattttgtc ttccataccc
     5821 actatcaggt atcctttagc agcctgaagg cctaagtagt acatattcat tgagtcgtca
     5881 aattcatata cattatgcca ttggctgaaa attacgcaaa atggcataga ctcaagatat
     5941 ttcttccatc atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat gtcggtgtca
     6001 tcatgcgcta cgctctatgg ctccctgacg tttttttagc cacgtatcaa ttataggtac
     6061 ttccatgtcg agtaagttag tactggttct gaactgcggt agttcttcac tgaaatttgc
     6121 catcatcgat gcagtaaatg gtgaagagta cctttctggt ttagccgaat gtttccacct
     6181 gcccgaagca cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc
     6241 aggcgccgct cacagcgaag cgctcaactt tatcgttaat actattctgg cacaaaaacc
     6301 agaactgtct gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta
     6361 taccagctcc gtagtgatcg atgagtctgt tattcagggt atcaaagatg cagcttcttt
     6421 tgcaccgctg cacaacccgg ctcacctgat cggtatcgaa gaagctctga aatctttccc
     6481 acagctgaaa gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga ctatgccgga
     6541 agagtcttac ctctacgccc tgccgtacaa cctgtacaaa gagcacggca tccgtcgtta
     6601 cggcgcgcac ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa
     6661 accggtagaa gaactgaaca tcatcacctg ccacctgggc aacggtggtt ccgtttctgc
     6721 tatccgcaac ggtaaatgcg ttgacacctc tatgggcctg accccgctgg aaggtctggt
     6781 catgggtacc cgttctggtg atatcgatcc ggcgatcatc ttccacctgc acgacaccct
     6841 gggcatgagc gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc tgctgggtct
     6901 gaccgaagtg accagcgact gccgctatgt tgaagacaac tacgcgacga aagaagacgc
     6961 gaagcgcgca atggacgttt actgccaccg cctggcgaaa tacatcggtg cctacactgc
     7021 gctgatggat ggtcgtctgg acgctgttgt attcaccggt ggtatcggtg aaaatgccgc
     7081 gatggttcgt gaactgtctc tgggcaaact gggcgtgctg ggctttgaag ttgatcatga
     7141 acgcaacctg gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc
     7201 tgcggtggtt atcccaacca acgaagaact ggttatcgcg caagacgcga gccgcctgac
     7261 tgcctgattt cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc
     7321 ggtaacgaaa gaggataaac cgtgtcccgt attattatgc tgatccctac cggaaccagc
     7381 gtcggtctga ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt
     7441 ctgagcgttt tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc cgatcagact
     7501 acgactatcg tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct gaaaatgagc
     7561 tacgttgaag gtctgctttc cagcaatcag aaagatgtgc tgatggaaga gatcgtcgca
     7621 aactaccacg ctaacaccaa agacgctgaa gtcgttctgg ttgaaggtct ggtcccgaca
     7681 cgtaagcacc agtttgccca gtctctgaac tacgaaatcg ctaaaacgct gaatgcggaa
     7741 atcgtcttcg ttatgtctca gggcactgac accccggaac agctgaaaga gcgtattgaa
     7801 ctgacccgca acagcttcgg cggtgccaaa aacaccaaca tcaccggcgt tatcgttaac
     7861 aaactgaatg caccggttga tgaacagggt cgtactcgcc cggatctgtc cgagattttc
     7921 gacgactctt ccaaagctaa agtaaacaat gttgatccgg cgaagctgca agaatccagc
     7981 ccgctgccgg ttctcggcgc ggtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc
     8041 gatatggctc gccacctgaa tgcaaccatc atcaacgaag gcgacatcaa tactcgccgc
     8101 gttaaatccg tcactttctg cgcacgcagc attccacaca tgctggagca cttccgtgcc
     8161 ggttctctgc tggtgacttc cgcagaccgt cctgacgtgc tggttgccgc gtgcctggct
     8221 gccatgaacg gcgtagaaat cggtgccctg ctgctgactg gcggctacga aatggacgcg
     8281 cgcatttcta aactgtgcga acgtgctttc gctaccggcc tgccggtatt tatggtgaac
     8341 accaacacct ggcagacttc tctgagcctg cagagcttca acctggaagt tccggttgac
     8401 gatcacgagc gtatcgagaa agttcaggaa tacgttgcta actacatcaa cgctgactgg
     8461 atcgattctc tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc agcgttccgt
     8521 tatcagctga ctgaacttgc gcgcaaagcg ggcaaacgta tcgttctgcc ggaaggtgac
     8581 gaaccgcgta ccgttaaggc agccgctatc tgtgctgaac gtggtatcgc aacttgcgta
     8641 ctgctgggta atccggcaga gatcaaccgt gttgcagcgt ctcagggtgt agaactgggt
     8701 gcaggcattg aaatcgttga tccagaagtg gttcgcgaaa gctatgttgg tcgtctggtc
     8761 gagctgcgta agaacaaagg catgaccgaa accgttgccc gcgaacagct ggaagataac
     8821 gtggttctcg gtacgctgat gctggaacag gatgaagttg atggtctggt ttccggtgct
     8881 gttcacacta ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc
     8941 agctccctgg tatcttccgt gttcttcatg ctgctgccgg aacaggttta cgtttacggt
     9001 gactgtgcga tcaacccgga tccgaccgca gaacagctgg cagaaatcgc gattcagtcc
     9061 gcagattccg ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc
     9121 gggacttctg gtgctggtag cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag
     9181 gaaaaacgtc ctgacctgat gatcgacggt ccgctgcagt acgacgctgc ggtaatggct
     9241 gacgttgcga aatccaaagc accgaactct ccggttgcag gtcgcgctac cgtgttcatc
     9301 ttcccggatc tgaacaccgg taacaccacc tacaaagcgg tacagcgttc tgctgacctg
     9361 atctctatcg gaccgatgct gcagggtatg cgcaagccgg ttaacgacct gtcccgtggc
     9421 gcactggttg acgatatcgt ctacaccatc gcgctgactg cgattcagtc tgcacagcag
     9481 cagtaatctc gtcatcatcc gcagctttac gctgcggata tctgaaccgg aaatagtcac
     9541 tatttccggt tttttattct cttaatttgc attaatcctt tctgattatc ttgcttaact
     9601 gcgctgcatc aatgaattgc gccatctcaa tttgcatact taccactttg ttttgtgcaa
     9661 gggaatattt gcgctatgtc cgcaatcact gaatctaaac caacaagaag atgggcaatg
     9721 cccgatacgt tggtgattat cttttttgtt gccattttaa ccagccttgc cacctgggta
     9781 gttccggtgg gcatgtttga cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa
     9841 acacgcaaag tcgtagatcc acactcattt cgcattctga ctaacgaagc aggcgaacct
     9901 gagtatcacc gcgtacagct gttcacgacg ggcgatgaac gcccgggtct gatgaacttc
     9961 ccgtttgaag ggttaacctc aggatcgaaa tacgggacag ccgttggcat catcatgttt
    10021 atgctggtga ttggcggcgc gtttggcatt gtgatgcgta caggaaccat tgataacggt
    10081 atcctggcgc ttattcgcca tacccgcgga aatgaaattc tctttattcc tgcgctgttt
    10141 attctgtttt cacttggcgg cgcaatattt ggtatgggag aagaggccgt cgcctttgcc
    10201 attattatcg caccgctaat ggtccggctg ggctatgaca gtattaccac cgtcctggtg
    10261 acctatatcg ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtt
    10321 gttgctcagg ggattgccgg cgttccggtg ctttctggct ccgggttgcg catcgtggtg
    10381 tgggttatcg ccaccctgat tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa
    10441 aagaatcctc ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga aaagcaggcg
    10501 gatgttgaac aacgtccgtt tacctttggt gactggctgg tattgattgt cctgaccgcc
    10561 gtaatggtct gggtgatttg gggcgtgatc gttaatgcct ggtttattcc agaaattgcc
    10621 agccagttct tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac
    10681 ggcatgacgg ttaataccat ggcttcatcc tttaccgaag gggcgcgaat gatgatcgcc
    10741 cctgccctgc tggtgggttt cgccaaaggg attttgctgc tggtcggtaa tggtgaagcg
    10801 ggtgatgcca gcgtgttaaa taccatcctc aacagcattg ccaatgccat tagcggtctg
    10861 gataacgcgg tcgcggcctg gtttatgttg ctcttccagg cggtatttaa tttcttcgtg
    10921 acatctggtt ctggtcaggc ggcgttaacc atgccgttac tggcaccgct tggcgatctg
    10981 gtcggtgtaa accgtcaggt taccgttctg gcgttccagt ttggtgatgg cttcagccac
    11041 atcatttacc cgacctcagc ttcgttaatg gcaacgctcg gtgtttgccg ggtggacttc
    11101 cgtaactggc tgaaggtggg cgcgacactg cttggactgc tgtttattat gtccagcgtc
    11161 gtggtgatcg gcgctcagtt gatgggctac cactaaaaat gttaagagcc gcaattgcgg
    11221 ctcttttttc attctgccgt ttcagtctct acggcttcat ttttggcatt gcgcttcatc
    11281 cacaacgcca gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct
    11341 tccggcgtca gccagcaaac ttcgctgact tcatcttcct gtagggcgaa gggaccgtga
    11401 gagacgcagc tgaacaacgc gccccagaca cggcaatttt tatcttcgaa atagaactgc
    11461 ccgtgctcgg caaaggggac accggcaatg cccaactctt cttccgcttc gcgacgcgcg
    11521 gattccagca gttgctcatc ggcctggact acaccgcctg cggtcgcatc taacatgccg
    11581 ggtaaaaagt cttttgtctc ggtacgacgc tggaccagaa ttttgcccat gccatcatgc
    11641 acgacgatgt aagttgcacg atgacgcaga cactgtgccc gcatttgttc ccggctggct
    11701 tgtgcaatga cttcgttctc ttcattgaca atatccaccc attcagtact tgccaaacga
    11761 cgctgttcca ttatcaggaa accttctttt tctggcgctc ttacggcgca ttttgagttg
    11821 tgggtaaatt acggattaat cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt
    11881 acactaagta cgtcattatc cagcatgcca taactcgccg gattaccgcc tttaggaata
    11941 ctcaccgagc cggggttgaa gtggaaaatc tccccccgct gttctgccac tggcagatgg
    12001 gtatgaccgt acaccagcac atcgttctgg tttaaagcag gtaggttttc cgggccaaac
    12061 agatgaccat gcgtcaaaaa cagacgttgt ttttccagta atacctgttg ccacggcgcg
    12121 gttatcggga aatgcaaaag catttgatcc acttcgctgt cgcaattgcc gcgcacagcg
    12181 ataaccttat gtgccacttc attaagccgt tcagcgactt tggctggcgc gtaaccctcc
    12241 ggtaaagcat tacgcgggcc gtgattcaac acgtcgccaa ggatcaccag ccactgggca
    12301 ccactttggg caaacaactc cagaacacgt tccgtcgccg gtaacgaccc atgaatgtcc
    12361 gatgcaaaca tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct atgatactgg
    12421 attctgtcgt tgctatcagc cagattgctt cgccgaaaga gcaataaaac gctggacaga
    12481 cgctcgctgc cactggaatg ttgcataatc caccaggcgt tctggcacct catcgccatg
    12541 tagtaccagg cggttaatca ttagcgccag atcagtatca gcaatgcacc attcaccaaa
    12601 taaattcggc tgacccagtg ctaacaaatg ttctgccatc gcgaacagtt tctctgcact
    12661 ggcttttccc tccgccgtta atggcgcttt tttcgcaccc gcaaaaacga catctgtcga
    12721 acgttcttcg cggatgggca tcagatcgct acgcagccag gcctgaatct gtcgtgcacg
    12781 cgcacgattt tctaaatcaa gcggataaat gcgttcccag gtcggtggcg caaatcgatc
    12841 ttccagatac tccgcaatgg cagaagattc actcaactca aaatcatcga tttgtaataa
    12901 cggcacacgg cgtgtctgac cgtaaccttg ccacgtcggc tgcaaatgtt caccgctgtc
    12961 gaggtcgatg gtcttgatat gaaacgacag gcctttttcc tgcaacgcca cccaggcgga
    13021 taacacataa ggggagaaaa aatgggcatc tgaccaaagc gtgatagcgg gtttactcat
    13081 tatgacctcg gcaataaagg gggctggcta tcaaactata gagccttttt gtcactgtca
    13141 cgtgacgaat actcacgcaa gtcgccgcgc tttctatact tgtggtgttc agatgattag
    13201 acaaccggag ctgcaatgat cgatctctat ttcgccccga caccaaatgg tcacaaaatt
    13261 acgctgtttc tcgaagaagc agggctggat tatcgcttga taaaggtaga cctggggaaa
    13321 ggcggtcagt ttcgcccgga atttttgcac atttcgccta acaacaaaat tccggcaatt
    13381 gttgatcatt ctccagccga tggcggcgaa ccgctaagcc tctttgagtc tggtgccatt
    13441 ttgttgtatc tggctgagaa aacaggactc tttttgagtc atgaaacgcg tgagcgcgcc
    13501 gccacattac agtggttatt ctggcaggta ggcggactgg ggccgatgct tgggcaaaat
    13561 catcatttta atcacgcagc ctcccaaacc attccttacg ctattgaacg ttatcaggtt
    13621 gaaactcagc gtctgtacca tgtactgaac aagcggctgg aaaactcgcc ctggctgggt
    13681 ggcgagaact acagcattgc ggatattgcc tgctggccgt gggttaatgc ctggactcgc
    13741 cagcgaattg acctcgcaat gtatccggca gtcaagaact ggcatgagcg gatccgttcg
    13801 cgccctgcca ccggtcaggc actgttaaaa gcacaactcg gtgatgagcg ttcggatagt
    13861 taacagaaac aggttctcgt gtattatttc atcctaagaa aaacaacgga gaacctgcaa
    13921 tggcacaacc tgccgctatt attcgtataa agaaccttcg tttacgtacg tttatcggaa
    13981 ttaaggaaga agaaattaac aaccgtcagg atattgttat caatgtgacg attcactacc
    14041 ccgccgataa agcgcgcacc agcgaagata tcaacgatgc gctgaattat cgcaccgtga
    14101 cgaaaaacat tattcagcat gtagagaata accgtttctc tttgctggaa aaattaactc
    14161 aggatgtgct cgatatcgca cgtgaacatc actgggtgac gtatgctgaa gtggagatcg
    14221 ataaactgca cgcgctgcgc tacgccgatt cggtatccat gaccttaagc tggcagcgtt
    14281 aaccgccata tcgggaggct gcatgaagat agtgatcacc ggagggacag gattaattgg
    14341 tcgccatttg attccacgtt tgctggagct gggccatcaa atcacggtaa tgacgcgtaa
    14401 cccgcagaaa gccagttctg ttctcggccc tcgggtggca ctatggcaag ggcttgccga
    14461 tcaaagcaac ctcaacggcg ttgatgcggt aatcaacctg gccggagaac cgattgctga
    14521 taaacgctgg actcacgagc aaaaagagcg tctctgccaa agccgctgga atatcacgca
    14581 aaaactggtc gatttgatta atgccagcga cacgccgccg tcggtactca tttccggctc
    14641 ggcaacgggc tattatggcg acttaggtga agtggtggtt accgaagagg aaccgccgca
    14701 taacgaattt actcataaac tctgcgccca ctgggaagaa attgcctgcc gggcgcaaag
    14761 tgacaaaacg cgagtgtgcc tgctgcgtac cggcgtagtg ctggcaccgg atggcggtat
    14821 tctcggtaaa atgctgccgc cgtttcgtct tggcctgggc gggccgattg gttccggtcg
    14881 gcagtatctg gcctggattc atattgatga tatggtcaac ggcattctct ggctgctgga
    14941 taacgagctg cgcgggccat ttaatatggt ttcgccctac ccggtacgca atgaacaatt
    15001 tgcccatgcg ctcggtcatg ctctgcatcg cccggctatt ttgcgcgtcc ccgcaaccgc
    15061 cattcggctg ttaatgggcg aatcttcagt actggtatta ggtggacaac gcgcgctgcc
    15121 taaacggctg gaagaagcgg gttttgcgtt tcgttggtac gatttagaag aggcgctggc
    15181 ggatgtcgtt cgctgatgtg gtttactgca aacatccgcc agttaactcc cggtgttaca
    15241 gggttagtgg ctttgcgcga taagatcgtc tggtgaaagt cgggtcacca tcataactaa
    15301 ctctcggtct aaacctctct ccagcatctc ctgagcaata cgcagggctt cttcgcgttt
    15361 gccaagaatt aatccatcat tccgcccttc ttcacgtaat ctgtcagcaa tagtcatcag
    15421 tttctccttt tcttgtggtg cgcgttccgc tatctcacca ataaatgcac ggaaacgctg
    15481 ggcatccccc gtttgtaata cgtaattaaa cagggctttt agctgtctgt cattagtgtt
    15541 ccctgtaact agcagcgaaa caatttggtc gactaatccc aacagatcgc gctgacgaat
    15601 atgtttctga attaactcca acagcgccat tttgcgatgt tgcataatct cgtcatccgg
    15661 caccacggta atatctacca gcggaaaagc cgatgaataa attttgcggg ctatagcagg
    15721 ctcggcaaat tcatcaagcc agcaaagtga ataaggataa ggacttctgc aaccatgata
    15781 aaacagcatc gggatcacca atggaagctc tttatagcct gcatcaagat ggttttgcat
    15841 cgccgcaatg gaatagcgca tcatgcgaaa agccattaat tcttccggct tactttggtg
    15901 ctctatcact acataaatat aacctgctcc ctcctgcgtt ttcacagacc acaagaggtc
    15961 ggaataatat tgccgcaggt cttcatcaat aaaactgttt ggttccagtt ttagcgttgt
    16021 taaatcacac agtttgcgca gcggcgcggg aagatgaata tcaataaaat cccgcgcggt
    16081 gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag ttgtcgatat
    16141 tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc acgattaata
    16201 cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaactatt tttagcggac
    16261 tgcaactttt acatttcagc ggaagctgac tcgcaaattc cccttgaaaa tactccggca
    16321 gcgttagcca ccggaggggg aattatttca gcgatccctt aaggaactgt tgcagacgag
    16381 ggctttgcgg gttaccaaat aactgttctg gcgcgccctc ttcttctatt ttcccctgat
    16441 ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc catttcgtga gtcactacca
    16501 ccatggtttt cccctcttct gccagttgct gcataatacg caacacttcg cctaccagtt
    16561 caggatcgag cgccgaggta ggttcatcaa acagtaaaac ttccggttcc atcgccagcg
    16621 cccgcgcgat agaaacacgc tgttgctgac caccggacag gtgcaccggg tatttcccct
    16681 gcgcacgttc gtctatcccg acttttgcca gatatttcac cgcccgctcg cgcgcttcct
    16741 gcttgctcag gcccaacacc tgaatcggcg cttccatgac gttttccagc accgtcatat
    16801 ggctccagag attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt aagcgcagct
    16861 gatttttatc ggcgactttg agttgaccat ctttgtcgcg caccagattg atcgtctggc
    16921 cattgaccac gatcgatcct tcgctcggtt tttcgaggaa gttaatgcag cgcagaaagg
    16981 tacttttccc cgatcccgac gatccgatga tgctaattac atctccggca ttcgcttgca
    17041 gtgaaacccc tttcagcact tcgtgttcgc cgtagcgttt atgcaaatcg ataacgttta
    17101 atttattctc ggacatcgta ttctcagtgc gttgaagaag gtttcacatg ctgcaaccag
    17161 cgtttttccg ctctgcgaaa gaggctgatc aggacataag agatgattaa atagagcacc
    17221 gcggcaatgc cgaaggcggt aaaaggttga tatgtggcgg cgttaatatc gcgggcaatt
    17281 ttcagcagat ccggcaccgt ggcagtaaat gccagcgcag tagagtgcag catcaggatc
    17341 acttcgttgc tgtacgccgg taacgcaata cgcagcgcag aaggcaaaat aatgcagcga
    17401 tacattttaa aggtcgagaa gccataggct ctggcggctt caatttcccc atgcggcact
    17461 gaacggattg ccccggcaaa aatctcggta gtgtaagcgc aggtgttaag cgtcagcgcc
    17521 agcacggtac agttcagacc actgcgaaaa aaggcgttaa ggaactcggt tcccttaaca
    17581 atctccagcg tgtacatgcc ggaatagaac accagcaact gaacatacag cggcgtaccg
    17641 cgaaaaatat aggtaaataa ccagattgga aactggatgt atttattact tgagacgcga
    17701 ccaatcgcca gaaacagcgc cagaactccg cctatcacta ccgacaaaat aagcagccac
    17761 agggtgatcg ccacgccagt aaagcgataa ccgtcggtcc acagcagtgg tttccagtat
    17821 tcatgtaaga tttcgatcac aggtcagccc tcttcacacc cacggagtag cggcgctcaa
    17881 ggaacagcag cacaccattg gaaacagtgg tgaaaaccag gtaaatcacg ccacagacga
    17941 tggcgaaata gaacggttcc caggtacttt tgcctgccag ctgagtggct ttgaccacat
    18001 cttccaggcc gagtaacgaa acgagtgcgg tagatttgag gatcacctgc cagttgttgc
    18061 caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat gatccgtcga aacacttgcc
    18121 cacgagtaaa accgaacgcc gtcgccgcct ctatgtgtcc tttcggcact gccataaacg
    18181 cgccacgaaa cgtttcggta aaataagcac cgtagataaa accgagagtg ataatacctg
    18241 cgaccatcgg atcaatatca atctgcccga cgcccatcgc ctccgtcacc gtgtttagcg
    18301 caatctgcaa accgtagaaa atcagcagca tcaacaccaa gtccggcacg cctcgaatca
    18361 gcgtggtgta cccttcgaaa atcagccccg aaagccgatt ttgcgagagc ttaccgccag
    18421 cgccaattaa accgataatt acagcgagca ctacagagct gatagccagc tccagcgtga
    18481 cgagcgcacc ctgtaaaata acacctgaaa acccatacaa catgctgcct gtcctgtcgt
    18541 gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc gaagggagcg gactttcttt
    18601 caccgatggc gattaaccac cataaacatc aaaatcgaag tactttttcg ctaatttctc
    18661 gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc gcagttcgtt
    18721 atcttcttta cgcaggccca tgccggtccc tacgccaaac agtttttcat ctttaacaga
    18781 cggaccaccg aatttgtaat ctttaccgac gggttgtttg aggaaacctt cgctggcagc
    18841 gacctcatcc tggaacgcgg catcaatacg tccggcagtc aggtcagaat aaatgttgtc
    18901 ctgcccctga taagaaacga tttcaatgcc ttttggtgcc caatgttcgt taccgaacgt
    18961 ctcctgggtg gtgccctgca atacgcctac ccgtttgcct ttcagcgact cgactgtcgg
    19021 ctgaatgtcg gaatttttcg ccaccaccag acgagaatcg gcagcgtaca gtttgtcggt
    19081 gaaggcaatt tcttgctgac gtttttccgt aatggaaagc gatgacatga tggcgtcaat
    19141 cttcttcgct tttaaggacg ggattaacgc atccagtggg ttttcgacaa acgtacattg
    19201 cgtattgatg cgtttgcaca actccttcgc cagatcgata tcgaagccaa ccagttcgcc
    19261 ttgtgaattt tttgattcaa atggcgcata ggtcggatcg gtaccgatgc ggatgttttg
    19321 cggaatcgca gcaaacgccg cagttgcgct ggagaaggcc agaaccagag agagcgatag
    19381 caccagtttt ttcataatgt cctcaactga cagtcttttt atagggaata tttacaggtt
    19441 tgttacactt atcgtgccat aaaattgacc atcaaggcaa aatatcccgc cccgttagac
    19501 gtattatttt tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga tagtgcaaca
    19561 aacctggcgt gcaccatgat gatcattcca tcaggtacag tttccctgcg acgtatcagt
    19621 caccgtagac attaaagtcg aaatactttt tcgccatctt gtcgtaggta ccgtcctgac
    19681 gcagctcgcc aagcgcctta ttaaacgccg ccgtcagttc agcatcatct ttacgtagcc
    19741 ctacaccggt accgtcaccg aagtattttt tgtctctcac tgatgagcca gcaaaggcga
    19801 aatctttacc agcaggttgc ttgaggaaac cttcgctggc cgccacttca tcctgcagtg
    19861 cggcatccag acgtcctgca gccagatcgg aatagaccaa atcctggttg gcataggcca
    19921 ccacatccac acctttgcta cgccaggtct cgttagcgta agcttcctgg gttgatccct
    19981 gcagcacacc cacatgttta cctttcagtg aatccagcgt tggctgaatc ggcgaacctt
    20041 tggcggcaat caaacgagaa tctgcggcgt acaacttgtc ggagaaagca atctcctgct
    20101 gacgtttatc ggtaatggag agcgacgaaa taatggcgtc gatttttttc gctttcagtg
    20161 aggggatcag cgcgtcaaag tcactggcaa cccaggtaca tttcacctgc atccgtttgc
    20221 acatctcgtt accgagatcg atatcaaagc caacaaagtc acctttggca tctttcgatg
    20281 agaacggcgc ataggtggta tcggttccga tacgtaccgt ctccggtagc gccgcatagc
    20341 tggaagccac tgcggagaga ccgactaaca aagacagagc gagaatcgac ttcttcatac
    20401 ataaccctca agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg tttgcaggct
    20461 ctttcatgca ggtcttatgc catctttacg acaacagtaa cattcaacgt taaatatgtt
    20521 aataagacgt tgcattattg tcctaaagtt gaagatagca ggtatggcgg ttgaatcgca
    20581 gcgtttcgat tcatcgaaag gaacaaaatg tcgaggattt gatcgcggtt gcaaaattgc
    20641 cctgaaacag ggcaacaatg aggttatgcg ccctgccagc gggcaaagag atcttcagga
    20701 agggttatcg caaactggtc aagaacacga ttaaccgtct gatttatcac atcatcaagg
    20761 gattgtgggc gatgataaaa cgccggaacg ggaggcataa tcaccgcacc gatttctgcc
    20821 gcctgagtca ttaaacgcag atggcctaag tgcagtggcg tttcacgcac gcagagcacc
    20881 aacgggcgac gctctttcag caccacatct gccgcacggg tcagtaagcc atcagtatag
    20941 ctatggacaa tgccggacag ggttttgatt gaacagggta aaatcaccat ccccaacgtc
    21001 tggaaagaac cggaagaaat gctggcagca atatcgcgcg catcgtgtgt cacatcagct
    21061 aacgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg gcgcgctgcc
    21121 tggctcatta ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg taataaacgc
    21181 acgccataaa tcgcgccgct ggcaccgctg atgcctacaa tgagtcgttt cataaatagt
    21241 tgccctgtca gacttgcagg cagactttgc aggatttcga cgggagttgc aagtcagggt
    21301 gccagaccgg caccctgagc gaaggcatca tccttcgtta tgcatttcga gattttccac
    21361 ttcgttctga cgttgcactg ctttggcgtc gtcattacgt aaagtatcga ggaaatcgag
    21421 gtagccctga tcaacgtctt tggtgacgta gacgccgttg aataccgagc attcaaactg
    21481 ctggatatcc gggttttcag cgcgaacggc ttcgatcaga tcgttcagat cctggaaaat
    21541 caacccgtca gcaccgatga tctggcgaat ttcatctact tcgcgaccgt gagcgatcag
    21601 ttccgtggcg ctcggcatat cgataccgta aacgttcggg aagcgaattt ccggtgccgc
    21661 agaagcgagg tacactttct tcgctccggc ttcgcgtgcc atctcgataa tctgctcaga
    21721 agtggtgcca cggacaatgg agtcgtcgac cagcaagacg tttttatcgc ggaactcggc
    21781 gcggttggcg ttcagtttac ggcgcacgga cttacgacgc agctgctggc ccggcatgat
    21841 gaaggtgcgg ccaacatagc ggtttttaac gaagccctgg cggtacggtt tgcccagaat
    21901 acgagcaatt tccagcgcga tatcacacga ggtttccgga atagggatca ccacgtcgat
    21961 atccagatct tcccattcgc gggcaatttt ctcgcccagt ttggtgccca tattcacgcg
    22021 cgcgctgtaa acggaaattt tgtcgatgaa cgagtccggg cgggcaaagt atacatactc
    22081 aaacaggcac ggattgctga ccggattgtc agcacattga cgggtaaaca actgcccttc
    22141 ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc aggaaatcaa agcccagcgt
    22201 atcgagcgca acgctttcgg aagcgaccat atattctgta cggttctcgt caatatcacg
    22261 ttttcccagt accaacggac gaataccgtt tggatcgcgg aaagcaacca taccgtggcc
    22321 gatgatcatc gccacacagg catacgcgcc gcggattaag cggtttgtag cagcgatggc
    22381 ggcgaaaatg ttgtcggctt ccagcggata gtggcggaag ttgtccagtt cgctggcaaa
    22441 gatattaagc agaatttccg agtcggaagt ggtgttgatg tggcggcgtt tttcttcaaa
    22501 cagtttttta cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa gcgtaatgcc
    22561 atacggggag ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc cagccgtagg
    22621 gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc gagcttcaaa
    22681 tacatcgctc accagcccgt tcgctttacg caaacggaag cagttattgg catctatggt
    22741 gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat cataaatcga
    22801 ctggttaacc ggcataacac cggcgatacc gacaataccg cacatacgtc tttttcctcg
    22861 ttaagccaca tctcagagca cttacgctct gggcaagaaa cttgacgagc tttgcagata
    22921 atcaaaaaac catctgatga taaaactgaa ctgcgggatc agctgtgatt tgctccagtc
    22981 ttcgcttttc gacaccccgg taaaggagtc gagaaagaag agaatggcgg caacaatcaa
    23041 cacaccgcgc aacgcaccga aacagacgcc cagcacccga tcggtgcctg acaaccccgt
    23101 tttctctacc aactggccta tcacgaagtt cacgatagca ccaacgatca gggtagcgat
    23161 aaacagtacc gcgatggcaa tcccatttcg aaccagttcg tcttcaaagc ccgtaaacca
    23221 gactgacagg taagtgtagt aatgactggc aacaaagaaa gcacaacccc atgtcaccag
    23281 cgataacgct tcacgaacaa agccgcggat caggctaacc agagaggaaa aagcaatcac
    23341 cgcgattatg gcgtaatcaa tccagaccat atgtgtccca cgattttacg ccctgtcatc
    23401 ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc gtagggattt ccttcccgcg
    23461 catcaataaa aatggcgctg aaaaaacatt caacgccatc gactttttat gcctttgcgg
    23521 catcgggcaa tgcgtgtcgg atgcggcgtg aacaccttat ccgacctacg gttctacccc
    23581 tgtgtaggcc tgataagacg cgccagcgtc gcatcaggca agaccgtatt aattcggcgt
    23641 atagcccatt accacgccac ttaagccaga aagttgcttc aactcaccca gcgatccttt
    23701 cagcttatct ttcgaggcat caggcccaac cagaatacgg gtaattttac cctgcactgg
    23761 cgtggatggc gacgtataaa cccgataacc ggcaccgcgc agcttaccga caatctcatt
    23821 cactttatcg gcatttttca gcgcgcccag ttgcacaaca taggctttac ccgttggcgc
    23881 tgctttttct tccacgaccg gcttcgacgc aggtggtgct tcaaccttcg gtttaggcga
    23941 ctccaccggt ttcggcttcg gtggggcgac cggtgcaggt tccggttcaa actcggtgtt
    24001 attgactgca atagtggccg gatcgagcga cggtgctgcg gcatcacctg cccgcacctc
    24061 ttccgctgcg ccttccggcg gctgcgtcgg cagcgcctgg gtggcggctg gcatcatatc
    24121 aggctcatcg cgatcgcccg ctttcggcac cagcgggata gccgcgaact catcctgata
    24181 atgttttttc tgcccgtcca gcagccctgg aagtacaatc acccccagcg ccaccagcac
    24241 gatcgtgccc actaaccgat tctgaaactt acttgccacc gcttctcctc gcgtcaatca
    24301 cttccatgac atgtgcgacc gtgtggaacg aaccacacac cagcacggta tcttccgctt
    24361 tagcgtccgc cattgcggca tcccatgcct gcgccacgct atcaaatgat ttgccgttac
    24421 ccaaatgctc aagcagttgt tctgccgtgg caccgcgcgg cccttccagt ggcgcacaat
    24481 accagtcatc aaccacgctt ttcaaccagg ccagagttcc ggcaatatct ttatcatgta
    24541 gcataccgat aaccgccagc acgcgcccgt ttttcggtag cgctttcata cgcccggtga
    24601 gatattccgc cgcatgtgga ttatgcgcga catcaaaaat aacgcgtggc gactcgctca
    24661 caatctggaa acgtcccggc aaaattgcgc tggcaatccc gtcgcgaatg gcattttcac
    24721 tgacctccag cccgctggca cgcagtgccg ccagcgccgt tgcggcattc ggttgcggga
    24781 caagcggcaa cggcagattt tccagcgtgc cgtgagcatc gctaaacgcc cagtcatgat
    24841 cggtgacgga atagttccac tcaacgcccc gacgttgtaa cagtgcactt ttttcctgcg
    24901 ccacatcagc aatggtagaa ggcatttccg gctcaccgac aattgccggt ttttcgctgc
    24961 ggaagatgcc tgctttctcg cgaccaatgc tttcgcgatc tggccccagc cagtcggtat
    25021 gatccagcgc aatgctggtt actaccgcaa catcggcatc gacaatattg gttgcgtcca
    25081 gacgaccgcc cagccctact tccagaatca ccacgtcaag ttgtgcctgt ttgaacagcc
    25141 acaacgccga cagcgtaccg tactcgaaat aggtcaggga aatatcaccg cgtgccgatt
    25201 caatctccgc aaaagaggcg gtgtgggccg attccggcaa ttcctggccc tgcacacgta
    25261 cgcgctcggt ataacgcacc agatgcggcg aagtgtagac gcccactttg taccctgccg
    25321 ccatcagaat cgactccagc gtacggcaag tggtgccttt gccattcgta cccgcaacgg
    25381 taaacacaaa tggcgcaggt ttcaggacgc caagacgcgc cgcgacctgg ctcacgcgct
    25441 caaggccgag atcgatagtt ttactgtgca ggttttccag ataagaaagc cacgaagcca
    25501 gaggcgacgc ggcttgagga gtgcgtttga taatcatggt atccgctgat tcgttacggt
    25561 gagaatagca aaagggcaga gccagtggcc ctgcccttat cagttatcag gcctcaggtt
    25621 cctgatctgg taccggtggc accactacgc cttcacgcgg cgcttcagga ttcggcgctg
    25681 gcagattcat caacttcgcc agaatgctcg ccagtttcag gcgcatttcc ggacgacgga
    25741 cgatcatgtc gatcgcgcct ttctcgatca ggaattcact gcgctggaat cctggcggca
    25801 gtttttcgcg aacggtctgt tcgataacac gcggaccggc aaagccgatt aacgctttcg
    25861 gttcagcgat gttgagatcg cccagcatgg cgaaacttgc agaaacaccg cccatggtcg
    25921 ggtcggtcag cacggagatg tacggcaagc cgcgctcctg catttttgcc agcgccgcag
    25981 aggttttcgc catctgcatc agcgacatca gtgcttcctg catacgtgcg ccaccagagg
    26041 cggagaagca gatcagtggg cagttatctt ccagcgcctg ctcaacggca cgcacgaaac
    26101 gtgcacctac cacagacccc atggaaccgc ccataaaggc gaactcgaat gccgcagcga
    26161 caactggcat tccatacagg gtgcctttca tcaccaccag cgcatctttt tcgccggttt
    26221 ctttctgcgc agatgccaga cggtctttat acttcttgga gtcacgaaac ttcagcacat
    26281 ctttcggctc aagctcgcta cccagctcca caaggcttcc ttcatctaac aggctatgca
    26341 ggcgattacg cgctgtcata cgcatgtgat ggtcacactt cggacagacc tcaagattac
    26401 gttccagctc agcgcggtat aaaacctgac cgcagctatc acacttagtc cacacccctt
    26461 caggaatgct cgccttgcgg gtgggagtaa tgttgctttt aattcgttca atccagctca
    26521 ttagggacct ttctgtctga acctggttcg atgccagttt tatctttggg gacgcataat
    26581 gccatttttg cccccaacag accatgaatg ttgcacatta aaacataaca gcccgaaact
    26641 ttggataaaa aagtggtcga accgcggagt tactttttat tttgcggcgc gtgccgcagc
    26701 gcgtttgtgg cgaataaatt cgaaaacacc tggcaatatt gaaacaaaaa taatgccaac
    26761 aatcatcagt ttaaggttgc tctgaatgaa ggggagcgtt ccgaagaaat aacccgcgta
    26821 ggtaaaaatc agtacccata gcaacgcgcc gatgacgtta aaagcggcga aatgacgata
    26881 cgacatgtgg cccattcccg caacgaacgg cgcgaacgtt ctgacgatcg gcacaaaacg
    26941 ggcgagaata atcgttttgc cgccatgttt ctcataaaac tgatgagtct tatcaagata
    27001 actgcgacga aagattttcg aatccggatt actgaacagt ttgtcaccaa ataaccgtcc
    27061 aatggtgtag ttcaccgcgt cacccacaat cgcggcgatc agcatcagca ccaccatcat
    27121 atggacgttg agatcgttgg tttccagtga tgccaacgca ccggcaacaa acagcaatga
    27181 atcacccggt aaaaacggcg ttaccaccag accggtttca cagaacaaaa ttaagaacaa
    27241 aatggcataa acccagacgc catactccgc gaccagttct gccagatgca cgtcaatgtg
    27301 caggataaaa tcgatgagga aataaatcag gtccatatta gcctatgcct tgtactcgtc
    27361 atctttcagg ctgtaactac gttggctaca gcctgaaatg ttccgagcat tattcttgtt
    27421 agtccgccag aaatagcggg cccattggcg gttttggaag atcataccgg tcagggtaat
    27481 ccaccgcgac cagatacagc ccttccgctt ttgccgttgc tgccgccagc gttctgtcct
    27541 ttgccgccag caactctgct atccagctct ccggctggtt gtgggcaccg acttccatca
    27601 ggctgccgac aatattcctg accatatgat gtacaaaggc attcgctttg atatctacca
    27661 ccacataagg accgtgacgc gtgacattaa tatgcataac gttgcgccac ggggttcggg
    27721 actggcactg caccgcacgg aacgaggtga aatcattctc gcccagcaag cattgcgcag
    27781 cccgatgcat ccgttcagcg tccagcggtt cgtaaaaatg ggttacccct ttactcagta
    27841 ccgccgggcg cagccgatga ttgtagatga tgtagcgata acggcgagcc gtggcgctaa
    27901 atcgggcatg aaaatcatca ggtacagctt taacccaacg cacggcgatg tcaccaggta
    27961 aattcgcatt tacgcccaac gtccacgcag cgtctttgcg ctgtgcggtc gtttcgaaat
    28021 gcacaacctg cccggtaccg tgtacaccgg cgtcagtacg cccggcgcag aagacggtga
    28081 tgggttcgtt cgccacctgg gagagcgcct tttccagctt ctcctgcaca ctgcggactt
    28141 cattctgccg ttgccagccg taatacctac tgccgtcgta ctcaatgccc agcgcaattt
    28201 tataaactgg cggttgttgc tggtcggaca ttagtacaga tactcctgca ccagtttctc
    28261 ggcgattttt actgccatca gcgcgccgcc aaagcgaacg ttatcggcca ccgaccagaa
    28321 ctgaacttgc tccggcatac cgtagtcatt acgtacgcag ccaacagaaa gatgcggcgt
    28381 accagaagca tctcctacct gagtcgggaa ttcgttctct tcagagagca caatatcttc
    28441 gccctgagca aacgcatcac gcgcttcttc tgccgccagc ggacgcagtg cttcaaagtt
    28501 gaccatctgg gcatgaccgt agaataccgg tgcctggacg acgcttgccg aaatcatcag
    28561 cccttcgtcc tgcaggattt tgcgtacttc gtcaacgata cgacgttctt cacgcacgct
    28621 accttcgcta tccggcagta acggcagcat gttgaacgcc agctgacgcc cgaagaaatc
    28681 ttcttcgtca atcggaatgc cattgagtaa tttagcactc tgccccgcta acgcatcgac
    28741 cgcttttttg ccctgggcgg aggctgaaat caggctggta acgctgatac gtgataaacc
    28801 gccctgatca attagcggtt tcagtgccgc cagcagctgg ctggtcagac tgtccggtac
    28861 ggcgatgaca ttccggttgc ggtaatcggt cagtacaaac gggtttactt ccggcactac
    28921 cagcggtacg tcaggttcga gagcaaacaa tccgctgctg tcgatcacca gacaacctga
    28981 gttggtcgct tcttcaaccc aggcagcggt agcttctttg cctgcgacaa aaaatgccag
    29041 ctgcgcctgc gtccagtcaa attcagcggc atcctgcacg gtgattgtct taccaccaaa
    29101 gcgcagttgt tcgcctgcgc tttcgttacg tgccagtgca taaatttccc caaccgggaa
    29161 ctgacgttca gccagcgttt caagcagggc ttcgcccaca gcgccagttg cgcccaggac
    29221 ggcaatgttc cagccttcag acatggtggt ttactccaga aatagcaaag ctccctgcca
    29281 atgttacagc agagagcatg aagaagagat taacgtgccg gatgatgaac ggcgttaaaa
    29341 cccagtttac acagcaatga tgccgcactg gcgtcatcac aaattacata cagagatgac
    29401 cattcacggc gctcaagata gtttttgcgc agtttatcga actcacccgg tatcccggcg
    29461 actttacgca gtggcgcatc atcgcggcgc acatcataca ccaaatgcac cagccttttc
    29521 agcgtcggtt gatcgagcgg accatgcagc gtaatgcgac caaactctgg cgcgggcagt
    29581 aatgtatcca gcgcaacgtg ttgttcatgc ccaatgaatt tgctataggc ttcaaacact
    29641 tgcgtagtac cgcgtgcttt accttccagg gtatagcctg caatatgcgg cgtgccgata
    29701 tccacttttg tcagcaattc cacattaagt tccggctcgc cttcccagac atccagcacc
    29761 acgcttaact tctggccttc attcaggcag gtcagcagcg cggtattatc gacgactgcg
    29821 ccacggcagg cgttaatcag aatcgctccg ggcttcaggc tacggatcag tttttcatcc
    29881 gccagatgtc gcgttttgta cggaccatct ttaaagagtg gcgtatggaa agtcagaata
    29941 tccgcatgct ggactaattc atccagcgag cggaaatcgc cctcatcccc acggtcggcg
    30001 cgaggcggat cgcaaagcaa ggttgtaatc cctaacgctt ccagtcgtgc ctgtaaacgc
    30061 cgaccaacgt taccaacgcc cacgatcccc acggtacgct cgtgcagtga aaatccatcg
    30121 cgttcagcaa gcatcagcag tgaggagaaa acatattcca ctactgcaat cgcattacag
    30181 ccaggtgcag cggaaaaacc aattcccgcc tgctttaacc atgtgtcatc aacatggtct
    30241 gtccccgcgg tggcagtgcc aacaaattta atgggttttc ctgccagcaa agattcattc
    30301 actttcgtga ccgaacgcac catcagcgca tccgcgtctg ccagttgagc cacggggatg
    30361 ggacgcccgg gaaccgcggt cacctcaccc aaacggctaa ataagtcgcg ggcataaggc
    30421 atattttcat caacaaggat cgttaataat ttttcagtac gcgacaatct ggccccccct
    30481 ttatcagcaa acaccctttt aaccccaccg tacaacatga aaaccatttc ataatcatac
    30541 acatacaaac gcaaccattt agacgggtga tactggaaag tatgtttctc agtatcaccc
    30601 ttaaagctat ttcagaccag acaaaatcac tctcccgtgg acaccgagtg gacacttacg
    30661 ccacctttca gcggattaag agccaccgca tcctgcaaat aatccggcgc aaaatgtgca
    30721 tacgccattg tttgctggat agtcgcgtga cccaggatcc tctgtagcgc aataatgttt
    30781 cccccgttca tcacaaaatg actggcgaac gtatgtcgca acacatgcgc cgcctgtcct
    30841 tttggcaaat cgggtttaac ccttttcagc accaggcaaa attcccggta cttcacatca
    30901 aataatcccc ccgtctcgct ggttttgatc gcctcacaaa ccgcctgcga gattggcacc
    30961 gttctcttac gaccgttttt ggtctcaaga aacgtcacac gattatgaac tatctgttca
    31021 ccacgaagct tactggcttc gccccagcgt gcccccgtac ttaaacacaa aagcgcaaca
    31081 cgccagtaat cgccctctag tgtatcgagc aataacgcca cttctttctg ggacaagaaa
    31141 gccatttctc gcggggacac ataaagcaca gagattcccc tgaccgggtg ctctgcatcc
    31201 catagtccta tttttttcag agcggtaaac attccggata accggttcat atacctgtta
    31261 gccgatgacg gcttcagtcc gtcagcgatc ctgcgagaac gccaggcaat gattttcagc
    31321 ttatccagat ccaccgcctg catatcagca ccaagttcat tgattatgtt gcgtaattgt
    31381 tttcggtctt cctccgcttt acgcctgtgc tggccgtgat acacccacca caactccagc
    31441 aaatcgttca acgtccggcg atcacggtag ccctgtatat attcccgctt ctcagcattc
    31501 gccatgatgt agcgctcagt ggccaccgct accgattttt tgttaaatac cttacgcacg
    31561 cgttttccct tgcgtccgtt cggcctgata tccagcaaat aacgaccatc ttcgagcttc
    31621 ttaatcgaca tggcgaagcc ctccaatgaa ccgttttaca atttcaccag cttcttccca
    31681 gcaatagtca gaccaggcaa aaagcaggtc caaccagttt tctggcctca gcggggtaat
    31741 tttcgggttg tttcggttga gtcctgattg ccctccaaat tgcaggagcc atcaagagag
    31801 agagccgggg ctatctgtcc ggcctccggc attgtttcat cagttgataa ccagtaggcg
    31861 tacttcttaa atttagggtg ttttgtaacc ttaagtaagg ccccctctgt tacttgcttc
    31921 cccctcacct catagttagt tactgtccca taaggcaacc ccacgcaatc cgcaaactcc
    31981 tggcgggtca taccttcagc ttcacgtatc aggcgaaatt tttcacccat gcttgacaaa
    32041 gatgccatat cgggcatatc ctccattgca aacgtgccac atcgggcaca cacataagaa
    32101 gccaaaataa gccactttgg gccattttga gccattcgaa cgaatcaagg agattaccac
    32161 aatgagagaa tcagagcttg agggcttcat tcaggtagcg ccatatccac ttgaggcggt
    32221 gccatatcaa ctattcgcca agatgattgg ccgcaaagaa tccaccgtca gaaccatgat
    32281 tgacgccgca aagctaccaa caattgactt tgtgaaaccg ggttcagtga aaactcgtgc
    32341 atcagaaaac tgggtatatc tgccagcatt taacgcaggt atgcgaaaag cattttttaa
    32401 tcaacccaaa gaacaacgcg atgcatggct gttatggctg ggtctgtaac gatatatcca
    32461 ggaatttttc aaaaccaata tatctactct gagagagttg tttgggcata aaaaagcaca
    32521 ggataactaa gctgataacc cactacagac acaaaaggaa acagccatga ctacacaaaa
    32581 agcaaaaacc ataattaaac gatacaacgt acgtagtggt gtggctggcg ttgtcttttc
    32641 cgatgtgaca cagcgacgca gcttccgcac tggcacagaa cgccacgcag cccgtttctc
    32701 aaccagtgca tcacgcagca acacgcacta cagcctgcag gaaacgcacg ccacgcctga
    32761 cgggcatctg gttaaacaaa ccggtaaaca cacctggctg attaatgatg ccggaattgt
    32821 catacacaga ggcgcacgca acccgattac cggaaatacc tgttactccc t
//
LOCUS       DAZKGF010000033        31722 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZKGF010000033 DAZKGF010000000
VERSION     DAZKGF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.033"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            21..1496
                     /gene="cdgI"
                     /locus_tag="ACHOQV_001529"
     CDS             21..1496
                     /gene="cdgI"
                     /locus_tag="ACHOQV_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP9629924.1"
                     /translation="MIRSTRISLGLFFNYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MASMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADQALREAKSEGGNKVIIHHI"
     gene            1677..3167
                     /gene="dgcJ"
                     /locus_tag="ACHOQV_001530"
     CDS             1677..3167
                     /gene="dgcJ"
                     /locus_tag="ACHOQV_001530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFP9629925.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPDLRGTLQTASTDCDTIVEAAALLPAFDQAVESNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3210..3713
                     /gene="yeaK"
                     /locus_tag="ACHOQV_001531"
     CDS             3210..3713
                     /gene="yeaK"
                     /locus_tag="ACHOQV_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFP9629926.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3714..3818)
                     /gene="yoaI"
                     /locus_tag="ACHOQV_001532"
     CDS             complement(3714..3818)
                     /gene="yoaI"
                     /locus_tag="ACHOQV_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFP9629927.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3988..4434
                     /gene="yeaL"
                     /locus_tag="ACHOQV_001533"
     CDS             3988..4434
                     /gene="yeaL"
                     /locus_tag="ACHOQV_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP9629928.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4391..5212)
                     /gene="nimR"
                     /locus_tag="ACHOQV_001534"
     CDS             complement(4391..5212)
                     /gene="nimR"
                     /locus_tag="ACHOQV_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFP9629929.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWARFFAMSERNLARLIVKETGVSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5309..6490
                     /gene="nimT"
                     /locus_tag="ACHOQV_001535"
     CDS             5309..6490
                     /gene="nimT"
                     /locus_tag="ACHOQV_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFP9629930.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSTMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVAAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6545..6892
                     /gene="yeaO"
                     /locus_tag="ACHOQV_001536"
     CDS             6545..6892
                     /gene="yeaO"
                     /locus_tag="ACHOQV_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP9629931.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLANIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6914..7168)
                     /gene="yoaF"
                     /locus_tag="ACHOQV_001537"
     CDS             complement(6914..7168)
                     /gene="yoaF"
                     /locus_tag="ACHOQV_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFP9629932.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7351..8376
                     /gene="dgcP"
                     /locus_tag="ACHOQV_001538"
     CDS             7351..8376
                     /gene="dgcP"
                     /locus_tag="ACHOQV_001538"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFP9629933.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8409..8507
                     /gene="yoaK"
                     /locus_tag="ACHOQV_001539"
     CDS             8409..8507
                     /gene="yoaK"
                     /locus_tag="ACHOQV_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFP9629934.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8510..8584
                     /gene="yoaJ"
                     /locus_tag="ACHOQV_001540"
     CDS             8510..8584
                     /gene="yoaJ"
                     /locus_tag="ACHOQV_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFP9629935.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8643..8891)
                     /gene="yeaQ"
                     /locus_tag="ACHOQV_001541"
     CDS             complement(8643..8891)
                     /gene="yeaQ"
                     /locus_tag="ACHOQV_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9629936.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(9039..9221)
                     /gene="yoaG"
                     /locus_tag="ACHOQV_001542"
     CDS             complement(9039..9221)
                     /gene="yoaG"
                     /locus_tag="ACHOQV_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFP9629937.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(9225..9584)
                     /gene="yeaR"
                     /locus_tag="ACHOQV_001543"
     CDS             complement(9225..9584)
                     /gene="yeaR"
                     /locus_tag="ACHOQV_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFP9629938.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSTEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9757..10395)
                     /gene="leuE"
                     /locus_tag="ACHOQV_001544"
     CDS             complement(9757..10395)
                     /gene="leuE"
                     /locus_tag="ACHOQV_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFP9629939.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10522..11445)
                     /gene="dmlR"
                     /locus_tag="ACHOQV_001545"
     CDS             complement(10522..11445)
                     /gene="dmlR"
                     /locus_tag="ACHOQV_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFP9629940.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11548..12633
                     /gene="dmlA"
                     /locus_tag="ACHOQV_001546"
     CDS             11548..12633
                     /gene="dmlA"
                     /locus_tag="ACHOQV_001546"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFP9629941.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKKIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVID
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            12884..14494
                     /gene="yeaV"
                     /locus_tag="ACHOQV_001547"
     CDS             12884..14494
                     /gene="yeaV"
                     /locus_tag="ACHOQV_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFP9629942.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHHV
                     EHYLPQTPVEVLEKTPVLPAGTVFKGDN"
     gene            14526..15650
                     /gene="yeaW"
                     /locus_tag="ACHOQV_001548"
     CDS             14526..15650
                     /gene="yeaW"
                     /locus_tag="ACHOQV_001548"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFP9629943.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15706..16671
                     /gene="yeaX"
                     /locus_tag="ACHOQV_001549"
     CDS             15706..16671
                     /gene="yeaX"
                     /locus_tag="ACHOQV_001549"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFP9629944.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHLAELQYSDVDWQLHYCTRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLNIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16718..17833)
                     /gene="rnd"
                     /locus_tag="ACHOQV_001550"
     CDS             complement(16718..17833)
                     /gene="rnd"
                     /locus_tag="ACHOQV_001550"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFP9629945.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGISDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17915..19600)
                     /gene="fadD"
                     /locus_tag="ACHOQV_001551"
     CDS             complement(17915..19600)
                     /gene="fadD"
                     /locus_tag="ACHOQV_001551"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFP9629946.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDSEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19805..20386)
                     /gene="yeaY"
                     /locus_tag="ACHOQV_001552"
     CDS             complement(19805..20386)
                     /gene="yeaY"
                     /locus_tag="ACHOQV_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFP9629947.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(20425..21120)
                     /gene="tsaB"
                     /locus_tag="ACHOQV_001553"
     CDS             complement(20425..21120)
                     /gene="tsaB"
                     /locus_tag="ACHOQV_001553"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP9629948.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGADLPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDEKGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLTLCDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(21178..23088)
                     /gene="yoaA"
                     /locus_tag="ACHOQV_001554"
     CDS             complement(21178..23088)
                     /gene="yoaA"
                     /locus_tag="ACHOQV_001554"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP9629949.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            23220..23564
                     /gene="yoaB"
                     /locus_tag="ACHOQV_001555"
     CDS             23220..23564
                     /gene="yoaB"
                     /locus_tag="ACHOQV_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP9629950.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            23986..24285
                     /gene="yoaC"
                     /locus_tag="ACHOQV_001556"
     CDS             23986..24285
                     /gene="yoaC"
                     /locus_tag="ACHOQV_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFP9629951.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(24405..24584)
                     /gene="yoaH"
                     /locus_tag="ACHOQV_001557"
     CDS             complement(24405..24584)
                     /gene="yoaH"
                     /locus_tag="ACHOQV_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFP9629952.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HAGERIVARFEDEDE"
     gene            24658..26019
                     /gene="pabB"
                     /locus_tag="ACHOQV_001558"
     CDS             24658..26019
                     /gene="pabB"
                     /locus_tag="ACHOQV_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFP9629953.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSENEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEGFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHTSDLLRAAFPGGSITGAPKVRAMEIIDELEPHRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26022..26600
                     /gene="nudL"
                     /locus_tag="ACHOQV_001559"
     CDS             26022..26600
                     /gene="nudL"
                     /locus_tag="ACHOQV_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFP9629954.1"
                     /translation="MEHRSLTLDDFLSRFQLLRPQMNRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            26784..28148
                     /gene="sdaA"
                     /locus_tag="ACHOQV_001560"
     CDS             26784..28148
                     /gene="sdaA"
                     /locus_tag="ACHOQV_001560"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP9629955.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            28279..29877
                     /gene="pdeD"
                     /locus_tag="ACHOQV_001561"
     CDS             28279..29877
                     /gene="pdeD"
                     /locus_tag="ACHOQV_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9629956.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDHALIKGSPVLIQWTPAAGSSK
                     AGVMEMINIDLLAAMLLEPQLPQISSASLTVDDRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLLRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(29881..31437)
                     /gene="yoaE"
                     /locus_tag="ACHOQV_001562"
     CDS             complement(29881..31437)
                     /gene="yoaE"
                     /locus_tag="ACHOQV_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFP9629957.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(31425..31583)
                     /gene="yoaL"
                     /locus_tag="ACHOQV_001563"
     CDS             complement(31425..31583)
                     /gene="yoaL"
                     /locus_tag="ACHOQV_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFP9629958.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
BASE COUNT         7828 a         8080 c         7712 g         8102 t
ORIGIN      
        1 gaaaacaatc attcggaaaa atgattcggt caacacgtat ttccctgggg ttattcttta
       61 attatttttt atcgttaacg aaaattgatc ttggtcaaaa ctatatatct ctgccatcaa
      121 taaaatccag cactcacatt gctctccttt ttatggcttc tatgggtaca caaaaattaa
      181 aagctcaagg cttttttatt ttcagcttat tgctgacgtt aattttattt tgcattacta
      241 ccttatataa cgaaaacacg aatgtaaaac tcatcccaca gatgaattac ctgatggttg
      301 ttgtggcttt gtttttcctt aacgccgtca ttttcctttt catgttaatg aagtatttca
      361 ctaacaaaca aattttacca acactcattt taagccttgc atttttaagt ggccttatct
      421 atttagttga aaccattgta attatccata aaccaatcaa cggcagtaca ctgatccaga
      481 caaagtcgaa tgatgtttct attttctata ttttccgcca actcagtttc atttgtttaa
      541 cctcgctggc gctcttttgt tatgggaaag acaacatcct tgacaacaat aagaagaaaa
      601 ctggcattct gttgctggcg ctgattcctt ttttagtttt tccccttctg gcacacaatc
      661 tgagcagtta taacgctgac tattctttgt atgtcgtcga ttactgtccg gacaaccata
      721 ctgcgacctg gggaatcaac tatacaaaaa tattggtttg tttgtgggca tttttactgt
      781 tctttattat catgcgtaca cgattagcca gcgaactatg gccgttaata gcattattat
      841 gtctggcatc gctatgctgc aacttacttc tactgactct ggatgagtat aattacacca
      901 tctggtatat cagtcgcggg attgaagttt ccagtaaact gtttgttgtg tcttttctga
      961 tttataacat ttttcaggaa ctgcaactct ccagcaaact ggcagttcat gatgtactga
     1021 ccaatattta taatcggcgc tactttttca acagcgtaga gtcattattg tcgcgacctg
     1081 ttgttaagga cttttgtgtc atgctggttg atattaatca gttcaaacgc atcaatgccc
     1141 aatggggaca tcgtgtgggt gataaagtgc tggtatcaat tgtcgatatt atccagcaaa
     1201 gcatccgccc cgatgatatt ttagcgcgac tggagggtga ggtgtttggc ttgctattta
     1261 ccgaactcaa tagtgcccag gcaaaaatca ttgcggaacg tatgcgtaaa aatgtcgaac
     1321 tcctgaccgg ctttagtaac agatatgatg ttcctgagca aatgaccatc agtattggca
     1381 cggttttttc aacgggtgac acacgtaata tctcgcttgt catgacggaa gcagatcaag
     1441 ccttacgcga agcgaaaagc gaggggggca acaaagtgat tattcatcat atttaagtgc
     1501 aaaaatattc agagccatgc tttttgcgtg gcttttgcat acaatttatt actattaccc
     1561 ctaaatttcc cctccccagc ggcgcagaga tgagtataat tagcgcccct gtgccaggcc
     1621 gcaatcgaac tttatctggt tttctcgttt cactaaccga aggagtgcca tttatcatga
     1681 aattgcacca tagaatgctc cggcatttta tcgccgcaag tgtcattgtg ctgacatctt
     1741 ccttccttat ttttgaactt gtcgccagcg acagagcaat gagtgcctat ctgcgctata
     1801 tcgtgcagag agcagactcc tcctttcttt atgataagta tcagaatcag agtattgccg
     1861 cgcatgtgat gcgcgctctc gctgctgagc agtcggaagt gtcgccagaa cagcggcgcg
     1921 ccatctgcga ggcttttgag tctgccaata atacccatgg cttaaacctg actgcccata
     1981 aatacccgga cttacgcggc acactacaaa ccgcatccac tgactgcgac acaattgtgg
     2041 aagctgcagc actattaccc gcttttgatc aggcagtgga aagcaaccgc caccaggatg
     2101 attacggttc aggtcttggg atggccgaag agaaatttca ctattatctc gatctcaatg
     2161 accgctatgt ctatttttat gaaccggtta atgttgagta ctttgcgatg aataactggt
     2221 ctttcctgca gtcaggaagt attggcatcg atcgcaaaga tattgaaaag gtatttaccg
     2281 ggcgtaccgt tttatcgagc atttaccagg atcagcgtac taaacagaac gtgatgagtt
     2341 tgctgacgcc ggtatatgtc gcagggcagc taaaagggat tgtgctgctg gatattaaca
     2401 aaaacaatct gcggaatatc ttttacactc atgaccgccc tctcctctgg cgttttctca
     2461 atgtcacgct aaccgatacc gattcggggc gcgacattat catcaaccag agcgaagata
     2521 atctgttcca gtatgtcagt tacgtccatg acttaccggg cggcattcgt gtctcgttat
     2581 ccattgatat tctttacttt atcacgtctt cgtggaaaag cgttctgttc tggattttga
     2641 cggcgttaat tttgctgaat atggtgcgga tgcacttccg tttataccaa aacgtgtcgc
     2701 gagaaaatat tagtgatgcg atgactggac tgtataatcg caaaatttta acccctgaac
     2761 tggagcagcg gttgcagaag ctggtgcaat ccggttcttc ggtgatgttt attgcaattg
     2821 acatggacaa gttaaagcaa ataaatgaca ccctcggtca tcaggagggg gatttagcga
     2881 ttacgttatt agctcaggcg attaaacaat cgattcgtaa aagtgattat gccatccgac
     2941 tcggtggcga tgaattctgc atcattcttg tcgattcgac gccgcaaatt gcagcacaac
     3001 tgcctgaacg tatcgaaaaa cgtctgcaac atatcgcgcc gcagaaagag atcggcttct
     3061 cttccggtat ttacgcgatg aaagaaaacg atacgttaca tgatgcgtat aaagcttccg
     3121 atgagcgttt atatgtcaat aagcagaaca aaaacagccg ttcatgataa ccttctgtgg
     3181 ttgtttgctt gtaatctcag gagcatgaaa tgactgaaat ggctaaagga agcgtgacgc
     3241 atcagcggtt aatcgcttta ttatcacaag aaggtgctga cttccgcgtc gttacccatg
     3301 aagcggtagg taaatgtgaa gcggtgtcag aaattcgtgg caccgcttta ggccaggggg
     3361 ctaaggcact ggtatgtaaa gtcaaaggca atggtgtaaa tcaacatgtt ctggcgatcc
     3421 tcgcggccga tcaacaggcc gatctgagcc aactcgccag ccatataggc ggattacgcg
     3481 cttcgttggc cagcccggcg gaagtcgatg aactcacagg ctgtgtcttc ggcgcgatcc
     3541 cccctttcag cttccatcca aaactcaaac tggttgccga tcccctactc ttcgaacgat
     3601 ttgatgaaat cgccttcaat gcaggcatgc tggataaatc cgttattttg aaaaccgcgg
     3661 attatttgcg cattgcgcaa ccagaactgg tcaatttccg ccgcactgcg taactagcct
     3721 gtccgttcga taagaagaac ggacaaaacc agcacaacag caatggcaaa aaacgatgac
     3781 gtgataatca gtgtctcgac gaacatttga tcgttcatag tatgcccccg ggagatagtt
     3841 gttttcacgt tattgttagc gtgcgcaaat ggcagtttga tgacagttcg cctgattttt
     3901 tatgcaaaaa acgtaaagat tttctacttc cttcctgcag caagcgtaaa gtaagcaggc
     3961 ttattattct ttggcaagga aaccacgatg tttgatgtca ctttgctgat cctgctcggg
     4021 ttagctgcgc tgggctttat cagtcataac accactgtcg ccgtttcaat tctggtgtta
     4081 atcattgtcc gcgtcacacc gttaagcacc tttttcccgt ggattgaaaa acaggggcta
     4141 agtatcggga taatcattct gacgattggc gttatggctc ccatcgccag cgggacgcta
     4201 ccgccctcaa ccctgattca ctcgtttctg aactggaaat cgctggtggc aattgcagtc
     4261 ggcgttattg tctcctggct gggggggcgc ggcgtgacgt taatgggcag ccagccgcaa
     4321 ctggtcgccg ggctgctggt gggcactgtt ttgggcgtgg cgctgtttcg cggcgtaccg
     4381 gttggcccgc ttattgccgc gggtctggtt tcgctgattg tggggaaaca gtagttaatc
     4441 ccgcaatata tcggcctggc gtttgcccca ggcctttctt aaacatagta ataaaggccg
     4501 ttgttgaatc ataccccagc gtatgagcaa ctttctgtac agtatcgcct ttcactaatc
     4561 cctgaagcgc cataatcagc tgtaactgct gacgccactg gcgaaaactc acccccgtct
     4621 ctttcactat cagtcgggca agattgcgtt cactcatcgc aaaaaagcga gcccattgcc
     4681 ccaatgaccc ccactcgacg ggctctttcg ccatcatctc caccatcgcg cggattttag
     4741 gatgagaaga aacgggtagg tgcagctgtt gttgtggctg ctgagggagt tcatcaaaca
     4801 ggacctgaat taatctgcgg gtcatcggct cagcacgctg agtcgtcgtc ctgtttgcca
     4861 gggttaaaat taattcccgg cataagggcg atattttcaa tgtgcaacag gttgtcggca
     4921 ttgtcactgc gctgggttcg ataaacagaa aacagagttc agcgttagct gtcacttgat
     4981 tgctatgttc cacccctccc ggtatccaaa ccgcatattg tggcggcacc atccacaaag
     5041 cattttccac cgtacaggta attgcaccat gtagtgccag tatcaactgt cctttgcggt
     5101 gctgatgtac aggactggtt aactcatccg ttccggcgtg aatacaaaat gcgaccgcgg
     5161 cttcatggtg tcggtcgggt tcatagccat tgagattcaa cctgtgcatc attttgtccg
     5221 aatttagcga taatttgtca ttttagcttg attcaacata acaataaaaa cggtaaggta
     5281 cagcctcgtt tgtaacaatg agaagcatat gacctgttca acttcattaa gcggcaaaaa
     5341 caggattgtc cttatcgctg gcattctgat gattgccaca acattacgcg tcacctttac
     5401 cggcgcagca ccgttactgg atacgattcg ttccgcttac tcgctgacga cggcgcaaac
     5461 cggcttattg accaccctgc cattattagc ctttgcgcta atctcacctt tggctgcccc
     5521 ggtggcgcga cgttttggta tggaacgtag cctgtttgcc gcgttacttt tgatctgtgc
     5581 tggtatcgca attcgctctc tcccttcgcc ttacttatta tttggcggta cagcggtcat
     5641 tggcggtggg attgcattag gcaatgtctt actgccagga ttaattaaac gcgatttccc
     5701 tcattccgtc gccagactta ccggcgcata ttccctgaca atgggagctg cagcggcact
     5761 gggatcgact atggtcgtgc cgctggcttt gaacggtttt ggctggcaag gcgcgttgct
     5821 catgctgatg tcttttcctc tgctggctct ttttttatgg ctgccacagt ggcgaagtca
     5881 acaacatgca aatttgagta cctcacgcgc cttacatact cggggtatct ggcgttcgcc
     5941 gcttgcctgg caggtaacat tgtttcttgg gatcaactca ctggtctatt acgtgattat
     6001 tggctggctt ccggcgattc tcatcagtca cggctatagc gaagcacagg cgggttcact
     6061 gcatggtttg ctgcaactag ccacagcagc tcccggtttg ctgatcccac ttttcttaca
     6121 tcatgtgaaa gatcagcgtg gtattgcagc gttcgttgcc ttgatgtgcg cagtggccgc
     6181 ggttgggctc tgctttatgc cagcacacgc gatcacctgg actctgcttt tcggttttgg
     6241 ttccggcgca acaatgatac tggggttgac gttcattggt ctgcgggcaa gttctgcgca
     6301 tcaggcggcg gcactctcgg ggatggcaca atccgtcggg tatttgctgg cagcctgtgg
     6361 gccgccgctg atgggtaaaa tacacgatgc taacggtgac tggtccgtac cactgatggg
     6421 tgttgcaata ctttcactac tgatggcgat tttcggactt tgcgccggga gagacaaaga
     6481 aattcgctaa tatccggtgc tatagtgacg tggcaaatca tgcgtgaaag ggagaacaaa
     6541 tacgatgaat attcagtgca aacgcgttta tgatccggct gaacagagcg atggttatcg
     6601 cgtactggtc gaccgcctct ggccacgcgg tatcaaaaaa accgatttag cccttgatga
     6661 atgggataaa gaaatcacgc cgtcaacgga actgcgcaaa gcctttcacg gcgaagtcgt
     6721 cgattttgca acctttcgcg agcaatatct tgcagaactg gcgcaacacg agcaagaagg
     6781 aaagcggctg gcgaacatcg ccaaaaaaca gccgctgacc ctgctctact cagcaaaaaa
     6841 caccacgcag aaccatgcgc tggtgctggc agactggcta cgtagcttgt gattttagta
     6901 cgccatccgg cagttatttt tcaccagccg gatggtcccg tcgccacaat gcccattcat
     6961 caatcgtttc accgcccggt aatttgcaat tgttgctgac cccttgcgct gtctgcacag
     7021 gaatgagcgt cccgcccttc tgctggcaat agaccgacgc cggatttgcc ataccaatct
     7081 gcggcggttt aggtgcttct ggctgagaag gggttgaaca accagccagg accagcaagc
     7141 aaggcagaac aaaactgata attttcattt attgatctca catatttatc cagacttaga
     7201 gtatcgcggt atcgttttgt tttgcagcac tatttttatt acattcactc aaatcatatt
     7261 acgccttgtt tcatctttgt tgatgatgtt ttatcatgcc tgcaaagatt aaataatcag
     7321 catttacccg ccgtatcctg gagttgtttc gtgtcagatc agattatcgc ccgcgtctcg
     7381 caatcccttg ccaaagaaca gtcactggaa agcctggtcc gacagcttct ggagatgctg
     7441 gaaatggtca ctgatatgga atcaacctac ctgaccaaag tggatgtcga agcgcgcctg
     7501 cagcatataa tgtttgcccg taacagccag aaaatgcaca tcccggagaa ttttaccgtc
     7561 tcgtgggatt actcgttatg caaacgcgcc attgatgaaa actgcttttt cagcgatgaa
     7621 gtccctgacc gttggggcga ctgtattgcg gcacgcaatc ttggcatcac cacatttctg
     7681 agcacgccaa ttcacttacc ggatggatca ttctatggca cgctttgcgc cgccagcagt
     7741 gagaagcgcc agtggagtga acgcgcggaa caggttttac agttattcgc cggactgatt
     7801 gcacaatata ttcaaaaaga ggcactggtt gagcagctgc gcgaagccaa tgctgcgctg
     7861 attgcgcaat cgtataccga ctcgttaacc gggctaccga atcggcgggc gatttttgaa
     7921 aatctgacga cgctgttttc tctcgcccga catcttaacc ataagataat gatcgcgttt
     7981 atcgatctgg ataacttcaa attaatcaac gatcgttttg gtcataatag tggtgatttg
     8041 ttcctcattc aggttggcga gcgccttaat acgctccagc aaaatggcga agttattggt
     8101 cgtctcggcg gtgatgagtt tttggttgtt tcactgaaca acgagaatgc ggatatttcg
     8161 tctctgcgag aacgtattca acagcaaata cgtggagaat atcacttagg tgacgttgat
     8221 ttgtattatc ccggtgccag tcttggcata gtagaagtcg atcctgaaac gaccgatgca
     8281 gacagtgccc tgcatgctgc cgatatcgcg atgtatcagg agaaaaaaca caaacagaaa
     8341 acaccttttg tcgcgcatcc agcgctacat tcctgaggcg tattcacatc cttttgattg
     8401 gtgataacat gcgaatcggt attatttttc cggttgtaat ctttattaca gcggtcgtat
     8461 ttttagcatg gttttttatt ggcggctatg ctgccccggg agcataaaga tgaaaaaaac
     8521 aacgattatt atgatgggtg tggcgattat tgtcgtactc ggcactgagc tgggatggtg
     8581 gtaacgtcac atctagaaaa tagcaaaggc tgcctgtgtg cagcctttgt gcaatttacg
     8641 cgttaacttt taatctttct gtagataaac agcacgacaa tcgcgccaat aacggcaacc
     8701 acgaagctgc caaaattgaa gccatcgact ttaccaaagc caaacagcgt gctgatccat
     8761 ccgccgacta cggcaccgac tatccccagc aggatagtca taaagaatcc accaccatct
     8821 ttacctggca tgatccactt cgccagaata ccggcaataa gcccaaaaat aatccatgac
     8881 agaatgccca ttgtttcctc acttatctgt tttgcattag cgggttagtc gctgataaaa
     8941 agcatagcac aacatcgtga gggcaagatt tgtgacgagc atcacggagg atttttttgc
     9001 gatggcgcag aaattgcgcc atcaacgatc agtgataatt accaaccaca aacatcatgt
     9061 tcgttttccg tgtcataaga acgcacggta ttcaccagat cttttatcac ttcagccgcc
     9121 acttctggca ccagcaaagt catcggcgtc tctgtttcat aatcgacaga aacgccattg
     9181 ctgttattgg tgacggtcac ggtatacgtt gctttgccca tgattcattt cccgttatga
     9241 atgactttcc gttgttgcgc accttccatc aggacttcag gagcaacgaa gaagtcaatg
     9301 ttgaaataag tgtcgtcagt catggcttca atgttgtgcc acttttctgg agggaacaca
     9361 gcaaactgcc ccgcttcgat aaggatcact tgatcaggct ctgtactgtg ttcatcagcg
     9421 tagccgagat atttgaccgc cccatgcata acggaaaggc gtgggtaaac ccccgggcgc
     9481 gttcctttat caagatgacg ttcgaatatt ccggcaggcg cagtttgttt attccagaaa
     9541 ggcgttgagc gcgtatgaat ataattctgt gggatttgca gcatcctttt ccctccttcg
     9601 gtgaatgcgc tgaaaacggt ttatttcagc cgtttcaggg tacgcctgat aatttgcatt
     9661 ttaaatacca tttattggtt actatttagc accgtatcag agaagaatca gggaggatta
     9721 caggtgggaa gcccgcgaca acgcgggctg aatgcatcag gactggagcg tcgccagtcg
     9781 ggcggcgaaa cccacaaaca tcaaaccaat cagtgagttg cccactttag ccagtttctt
     9841 tttggtacgt atgtactgcg tgacaaaagc accagaaata atcaggaagc tcaaatagca
     9901 gaaactcacc agttccagcg tcgtcgccag aataaagaat gaaattcccg tatgtggggc
     9961 attaacatcg ataaactgta cgaaaaacga cacatagaac aaaatggctt tcggattagt
    10021 caggctcaaa attaacgcgc gtttaaaaat ggcaccgtat tggggctcat cggatttggc
    10081 ctcgttattt ttacccttca gggtcgcgta gagaatttta ctccccagat agagcaaata
    10141 aaacgcacca agataacgta caatgttgaa taatatcggg gtggtcttaa ttaatgtcgc
    10201 cactccagcc catgccagaa acatcaatac cgcatcgcca ataaatacac cgcaggccgc
    10261 aagataaccg cctttcatac cgctactgac gctatttttg agtacaaaca gggtatttgg
    10321 ccctggcacc aacacaataa aaatggcccc aaccagatag gtccagtaat tcagaacccc
    10381 gtattcagcg aacacattaa cctctttaat tatctttcga tcatgcgcga ttaaaggtga
    10441 atatgctaac caatctgtag cggcttagaa aggagaaaat caggttttaa cctgatatca
    10501 acccgataat tgaatcatta atcaggcatg ctccagtgaa aaattcgggt agtgctcggc
    10561 aaaatactgg cgtaaaaatt ctaccgttat ccgtactttc gctgacgtcg ccagccttga
    10621 aacataaacg gcccagacat tcgctggctg gtaatattcc ggtagcactt gtactaaatg
    10681 accactggca atgttttcgc taacatccca ccaggagcgc agagcaatcc cctgcccatc
    10741 gagacaccac tgatggacaa tttctccatg attagacgac agtgggccgg taactttaat
    10801 cgcatggtgg ccttctttat tgcgtaattg ccagacacca aaaggatgat cgcgctcttt
    10861 aatgaccagg cacggcaatg cagaaagatc ggttaaatgt ttcggcgcgc catgttgcgc
    10921 aataaattcc ggtgacgcgc agagaatgcg ataattggtc gccagtttgc gggcgattaa
    10981 attaggggcg atgtcatcgc caatgcgaat atcgagatcg acaccttcat tgaccaaatc
    11041 gaccaaccgg tcttccacat caaaacgtaa ttccagttgc ggatacgcct tcgccagcgc
    11101 tgataacgcc ggggccacca cctgtcgtcc aaaaccaaag ctgctgataa tacgcagcat
    11161 cccctgtggc acctgacgca cgtcagaaag ttcgtccatc atctgaccga catcctgcaa
    11221 aatccgctgc gcccattcat aaatccgctc tccttcttcg gtaatggtga cgcggcgggt
    11281 ggtgcggtgt aacaacacca cgtttagcgt ttgctccagc aaggcgatgc gcttgctgac
    11341 gaacgccgga gaaacgccca gttcttccgc cacggcggca aaaccagccc ggcgagccac
    11401 cagcataaag acgcgcaaat cgttcagcag cggtaaatta ttcatgattc gtgttttatg
    11461 tttcaccagt tacagggatt aattcctttt cagtcaatta taggatggtg atgttgtcaa
    11521 ttttgatggt caggaagtga gaacccaatg atgaaaacga tgcgtattgc tgcgatcccg
    11581 ggagacggga ttggcaaaga agtccttcct gaagggattc gcgtgttaca ggctgccgct
    11641 gagcgctggg gcttcgcctt gagttttgag caaatggagt gggcgagctg cgagtattac
    11701 agccatcacg gtaaaatgat gccggacgac tggcatgagc aacttagccg tttcgacgcc
    11761 atctattttg gtgccgtcgg ctggccggac accgttccgg accatatttc gttgtggggt
    11821 tcgctgctga aatttcgtcg tgaattcgac cagtacgtca acctgcgccc ggttcgtctc
    11881 tttcctggcg ttccctgccc gctggcggga aaacagcctg gcgacatcga tttttacgtg
    11941 gtcagggaaa acaccgaagg cgaatattcc tcgctcggcg gcagagtgaa tgaaggtaca
    12001 gagcatgaag tcgtcattca ggaatcggta tttactcgtc gtggtgtcga tcgtattttg
    12061 cgctatgcct ttgaacttgc gcaaagccgc ccacgtaaga cgctaacttc tgccactaaa
    12121 tcgaacggtt tagccatcag catgccgtac tgggatgagc gagtggaagc aatggctgag
    12181 aattacccgg agatccgctg ggacaagcag catattgaca ttctctgcgc acgttttgtg
    12241 atgcaaccgg aacgcttcga tgtggtagtg gcgtccaatt tgtttggcga tatcctttcc
    12301 gatcttggcc cggcctgcac cggcaccatt ggcattgccc catccgccaa cctgaatccg
    12361 gaacgcactt tcccgtcgct cttcgagcct gtccacggtt ccgcgccgga tatctacggg
    12421 aaaaaaattg ctaaccctat cgccacaatt tgggccggtg caatgatgct cgattttctc
    12481 ggcaatggag atgagcgttt ccagcaagcg cataacggta ttctggccgc gattgaagaa
    12541 gtgattgatc acgggccgaa aacacctgat atgaaaggca atgccaccac gccacaggtt
    12601 gccgacgcga tttgcaaaat tattttgcgt taaggtcaaa ccagtttatt tgaaccgcgt
    12661 cactgacgcg gtttttttat tcgttctttg cagtaaataa cctgcgtcat ttcacctttt
    12721 attgtttccg tttcgtgttt tatggctttc cgtattctta attgtttaat ttatgtaaca
    12781 tgcaaataat caatttgtta atcacgacat acaactcatt accctgcaaa taattacaac
    12841 tggcaaaagc cagagacggg cttttatgcc caggagatga ctcatgatga gcaatgttaa
    12901 gaaaaaagat gtgccgctga taagcatcag cctggtggcc attcttttca tcgcagctgc
    12961 attaagcctt ttcccacaac aatcggccga cgcggccaac gcaatataca cttttgttac
    13021 gcgtacgtta ggttccgccg tacaggtatt ggttttgctg gcaatgggac tggtgattta
    13081 tttagccacc agtaaatacg gcaatattcg tcttggcgaa ggaaaaccgg aatacagcac
    13141 gctctcatgg ctgtttatgt ttatttgtgc cggtttaggt tcttctacgc tttattgggg
    13201 ggttgctgaa tgggcctatt attatcaaac gcctggatta aatatcgcac cgcgttcaca
    13261 acaggcactc gaatttagcg ttccctactc cttcttccac tggggcatca gcgcctgggc
    13321 aacttatacg ctggcctcat taatcatggc ttatcacttt catgtgcgga aaaataaagg
    13381 gctgagcctt tccggcatta ttgctgctat taccggcgtt cgcccgcaag gcccgtgggg
    13441 aaaactggtc gatttgatgt tcctgatcgc cactgtcggc gcactgacca tttcccttgt
    13501 tgtcaccgca gcaaccttta cccgtgggct ttccgcgctg accggtttac ccgataactt
    13561 taccgtacag gcatttgtga tcctgctttc cggcggcatt ttttgcctaa gctcgtggat
    13621 tggtatcaac aacggtttgc aacgtctaag caaaatggtt ggctggggcg cgttcctgct
    13681 gccattactg gtgctgattg tcggtccaac cgaatttatt accaacagca tcatcaatgc
    13741 catcggcctg accacgcaaa acttcctgca aatgagctta ttcaccgatc cgcttggcga
    13801 tggttcattt acccgcaact ggaccgtttt ctactggctg tggtggatct catacacgcc
    13861 tggcgtagca atgtttgtca cccgcgtttc ccgcggtcgt aagattaaag aagttatctg
    13921 gggactgatc ctcggcagca ccgtcggttg ctggttcttc ttcggcgtaa tggaaagcta
    13981 tgccattcac cagtttatca atggcgtaat caacgtccca caggtgctgg aaacactggg
    14041 cggcgagaca gctgtgcagc aagttctgat gtcgttgcca gccggtaaat tgttcctcgc
    14101 cgcatacctg ggcgtgatga ttattttcct tgcctcgcat atggatgcgg tggcctacac
    14161 catggcggca accagtacgc gtaatctcca ggaaggtgac gatcctgacc gtgggctgcg
    14221 tcttttctgg tgcgtggtaa ttaccctgat cccgctttca attcttttca ctggcgcgtc
    14281 gctggaaacg atgaaaacca ccgtcgtact cacagccctt ccgttcctcg tcattttact
    14341 ggtgaaagtc ggcggattta ttcgctggct gaaacaggat tacgccgaca ttccggctca
    14401 tcatgttgaa cattatctcc cgcagacacc ggttgaagtc ctggaaaaaa cgccagtgct
    14461 ccctgcggga accgtattca aaggcgacaa ctgagcgcca tcatcctaac gataaaggta
    14521 tccctatgag caatctgagc cctgactttg tactacccga aaatttttgc gctaacccgc
    14581 aagaggcgtg gaccattcct gcccgttttt ataccgatca gaacgccttt gaacacgaaa
    14641 aagagaacgt cttcgccaaa agctggattt gcgtcgctca cagcagcgaa ctggcgaatg
    14701 ccaatgatta tgtgacgcgt gagatcattg gcgaaagcat tgtgctggta cgcggtcgtg
    14761 ataaggtttt gcgcgcgttc tataacgtgt gtccgcaccg tggtcatcag ttgttgagcg
    14821 gcgaagggaa agcgaaaaat gtgattacct gcccgtatca cgcatgggca ttcaaactcg
    14881 atggcaacct ggcccatgca cgtaactgcg aaaacgtcgc caatttcgat agcgacaaag
    14941 cgcaactggt tccggtgcgt ctggaagaat atgccggatt cgtcttcatc aacatggacc
    15001 ctaacgccac cagcgtagaa gatcagttac ccggtctggg cgcgaaagtg ctggaagcct
    15061 gcccggaagt ccacgatctg aaactggcgg cccgctttac cacccgcacg cctgccaact
    15121 ggaagaacat tgtcgataac tatctcgagt gctatcactg tggtccggcg catccaggtt
    15181 tctccgactc cgtacaggtt gatcgttact ggcacaccat gcacggtaac tggacgctgc
    15241 aatacggttt cgccaaaccg tcagaacagt ctttcaaatt cgaagagggc acggatgcgg
    15301 cattccacgg tttctggctg tggccgtgca cgatgcttaa cgtcacaccg atcaaaggga
    15361 tgatgacggt catttatgaa ttcccggtgg attctgaaac taccctgcaa aactacgata
    15421 tttacttcac caatgaagag ttaaccgacg agcaaaaatc gctgattgag tggtatcgcg
    15481 atgtgttccg tccggaagat ttacgtctgg ttgaaagcgt acagaaaggg ctgaaatcgc
    15541 gtggctatcg tggtcagggg cgcatcatgg ccgacagtag cggtagcgga atttccgaac
    15601 atggtatcgc ccatttccat aatctgctgg cgcaggtgtt taaggactaa tgacatcggc
    15661 ggcggtattt tccgccgctg ggctgatttt tgatggagta cagcaatgtc agactatcaa
    15721 atgtttgaag tacaggtgag tcaggtggaa ccgcttaccg aacaggtgaa acgcttcacg
    15781 ctggtggcaa ccgatggcaa accgctgcct gcgttcaccg gaggaagtca catcatagtg
    15841 cagatgagcg atggtgataa ccagtacagc aatgcgtatt cactactgag ttcaccgcat
    15901 aacacctcta gttatcagat tgccgttcga ctggaggaaa actcgcgcgg cggttcccgc
    15961 tttttgcatc agcaggtaaa agtgggcgat cggttaacga tttcaacgcc taataacctg
    16021 tttgcgctaa ttccctcggc cagaaagcat ctgtttatcg cgggcggtat tggtatcacc
    16081 cctttcctgt cgcacctggc agagttgcaa tacagcgacg tcgactggca gctacattac
    16141 tgcacgcgaa atccagaaag ttgcgcattt cgtgatgagc tagtccagca tccacaggct
    16201 gagaaagttc acttacatca ctcatcaacc ggaacacgac tggaattagc gcgattattg
    16261 gcggatatcg aacctggcac acatgtttat acctgtggcc ccgaggcgct aaatgaagcg
    16321 gtaagaagtg aagctgcgcg tctgaacatc gccgccgata cgctgcactt tgagcaattt
    16381 gctatcgaag acaaaaccgg cgatgcattt accctggtgc ttgcccgttc cggaaaagag
    16441 tttgtggtgc cggaagagat gactattttg caggttattg aaaataataa agcagcgaaa
    16501 gtggaatgtt tatgtcgtga aggggtatgc ggaacctgcg aaacggcaat actggaaggt
    16561 gaagctgacc atcgggatca gtattttagc gatgaagagc gtgccagcca gcaaagtatg
    16621 ttgatctgtt gttcgcgcgc gaagggtaaa cgcctggtgt tggatttgta gtttgccgaa
    16681 gccggactga gcgcgcccag tccggcttcg gaagatttta ctgcggatat tcctgcaata
    16741 aattgtgtaa tgcttccgcc atcagctcac cacgccagcc ggaaatcagc tccggcaaat
    16801 tgttctgcgg tttcagtttc cagtgccagt tcagcagttg gttgatttgc cgacgcgatg
    16861 ccagcaattc ggcgctgatc ttgtgcgttt cgctcacgtc agtaatcagc gacttaatcg
    16921 ctttaaacgc tttacgataa cccggcatgt ccatcaggtt aagcatcggc tgcggtaagg
    16981 cctcttccgg caatgcctgc gctttttcca ccagcgccag cagcgtttta ccgtgaaagc
    17041 ggatttcgct accggataag cccaggctgt ccagttcgcc taaacttccc ggcatataac
    17101 gcgctacgga ccacaaatgc tcttcacgca cgacaaagtt cactgccaga tcgcgctccc
    17161 gcgccttgcg cagtcgccag tcggctaaca gttgcagaca ggccagttgg cgtgtgcgta
    17221 actgccaggc attggtgata tcacgccagg catcttccgg agcaacgact tcctgacgac
    17281 gcatttgcat caggcggcat tcatccagcg ccgcaggtag ccagccggag gcctccgttt
    17341 ctaccataag ctttgcggtg atcggtaaca gataccagac atccgccgct gcgtattcac
    17401 actgacgttc ggtcagcggt ctggccagcc agtcggtgcg cgattcactc ttgtccagcg
    17461 taacgccgga atactcttcc accatggaag cgaaacccca tgacatcggg cgtccgcaga
    17521 aggcagcaag gatttgcgtg tcaatcaagg gttgtggtaa ttcgccaaag acattgagga
    17581 acacttccag atcttcactg cccgcatgga gaaattttgt gatggacgga tcgcgcagga
    17641 tcgctttcag cggtgaccag tcagagatcc cgaggggatc aattagcgcc agatgctcac
    17701 catcaaaaag ttgaattaga cccagttgcg ggtaataggt gcgcgtacga acaaattcag
    17761 tatccagggc tatcgccgga aaggcacgga cggcttcaca caaagaagcc agcgcatcgt
    17821 ccgtggtaat catttggtaa ttcaaattgt attctcttta gtgggcgtca aaaaaaacgc
    17881 cggattaacc ggcgtctgac gactgactta acgctcaggc tttattgtcc actttgccgc
    17941 gcgcttcgtc acgtaattct cgtcgcaaaa tttttccgac gttagatttc ggtaactcat
    18001 cacgaaactc caccagcttc ggtactttgt agcccgtgag ctgacggcgg caaaaagtca
    18061 ccagtgactc ttcggtaagc gatggatctt ttttcacaac gaagattttc accgcttcac
    18121 cactggagcc ggaaggtacg ccaacagccg cgacttcctg tacgccagga tgctgcatga
    18181 cgacatcttc aatctcgttg ggatagacgt taaaaccgga aaccagaatc atgtcttttt
    18241 tacgatcgac aatgcgcagg aatccttctt catccatcac cgcgatgtcg ccggtgtgta
    18301 accagccatt tttgatgatt tcatcggtag catccggacg ctgccagtaa cccagcatca
    18361 cctgcggtcc tttgacacaa agctcacccg gttgaccagg tggtacttca ctatcatcat
    18421 catccaccag tttggcttcc gtcgacggca ccggcaaacc gatgctacca ctatgataat
    18481 caatatcata cgggttaacg ctcaccagcg gcgcacactc ggtcaggcca taaccttcca
    18541 gcagatactg tccagtcagt ttaacccaac gttctgccac cacttgctga acaggcatcc
    18601 ctccgcctgc ggaaagatgc agactggaga aatccagctg ctggaactct ttattgttca
    18661 gcaacgcatt gaacaaggtg ttaacgccag tgatagcggt aaacggatat ttcgctaact
    18721 cttttaccaa ccctggaata tcgcgcgggt tagtgataag caggttctgc ccccccagtt
    18781 cgataaacag caggcagtta atggtcagtg caaaaatgtg atacagcggc agcgccgtca
    18841 ccaccagctc tttgcccgga tgcaacagcg gaccataggt cgcgttaacc tgttccaggt
    18901 tcgccagcat attgcggtga gtcagcatcg cgcctttcgc cacaccagtg gtgccgccgg
    18961 tgtattgcag gaaagctaaa tcttccggca ccagttcggg tttgacgtac tgcatccggt
    19021 agccgttgtg cagggcgcta cgaaatgaaa tggcatctgg cagatggtat ttcggcacca
    19081 gacgcttgat gtatttaaca acgaaattga ctaccgtgcc ttttgccgta gatagctgat
    19141 cgcccatacg ggtcagaatt acgtgctgaa cggcggtttt atcaaccact ttttccagag
    19201 tgtgggcaaa gttagacacg ataacaatcg ccgatgcgcc gctatcgtta agctgatgct
    19261 caagttcacg cggggtatac aacgggttaa cgtttacgac aatcatcccg gcacgcaaaa
    19321 tgccaaacag cgccaccgga tattgcaata aattaggcat catcaacgca acgcgatcgc
    19381 ctttcttcag tcccaaccct tgttgcaaat aagcggcaaa cgcgcgactg cgttcttcca
    19441 gtttgcggaa ggtcatcacc tcccccatat tcacaaacgc gggttgatcg gcgtagcgcg
    19501 cgaccgactg ctcaaacata tctaccagag attgataacg gtcagggttg atctccgtcg
    19561 ggacgtccgc gggataacgg ttaagccaaa ccttcttcaa ttcttcacct ctaaaatgcg
    19621 tgttcgtcgt catcgcaacc ccaaatgata tacatgctgt taacataata ttaactcatc
    19681 ataccagctt gataattacc caacgaaaag gttgcgaagc gcgtcactat ttatttttat
    19741 ctttaccgta agaatgcaga aacagcggac cagccgctgt ttctttttct ttaaaaacaa
    19801 gcaattattc agttacaaca gtttgtactc tcgcggggcc gggattatac cagccccatc
    19861 caccgtagcc atagggccag ccacgaccgc cataaaacca tggatcaatc ggctgaggcg
    19921 gcataatcac ctgctgggtt aaatgccaac gtttgtaacc cgttacctgc atcaccataa
    19981 atttataggg cgtgttgccg attttgccgt caaccgcacc ggtgattggc ccaactaccg
    20041 taaccagttg tccacggaaa tctaccgggt ccagaaaacc gttcacatca gcataaatgc
    20101 gaccgcgaga aggttctccc agcgtcggtc tggctccgct gtccagtggt acggtggcaa
    20161 tttccaggcg ggttttccct tgctggtttt gtaccgcaac cactttgcca ccaaagcgtg
    20221 cctcctgacc aacgtacagc tgcggcgcac tcatcacccg aactaaatct tgttgcggcg
    20281 tgggactgct gcctttaatg gcgtccggca cagtaacaca accgctgagc attagcgcaa
    20341 acgtacctgc cagtatgcct ttgataacat tcttttgaac cgccatgatg cgactccttt
    20401 tttctcaggg catactctta agattcattc tttgcccgga agtttcttcc atgcgacgtt
    20461 gttacgtaaa taaaccggtt cggcatgttc caccgccacc gttttaccct cggcaaacat
    20521 ctgacacgca atcggaagca tatcttctgc agcgggcagt aacacttcgc catcgcacag
    20581 ggtcaaaccg ctctctttac cgagatccgg ccaggcttgc cagcctgtac ctaccgtcac
    20641 ccattccccg gaaagctgtt gcatccgttc atgaacgagt tcgggtttga gtacggcttc
    20701 ggtttcttca ccgtgccaga tacccttttc atcacgctga tattcggccc agtaaacttc
    20761 gcccatccgc gcatcaatag cagccagcac gcgggttgcg ccgtttttgc gccacgcccc
    20821 ttgcgccatt gtcatgagcg tggaaacgcc aatcatcggc agatccgcgc caagcgccag
    20881 cccctgcgca ataccaatac caatgcgcac gccggtaaag ctaccggggc cgcgcccgta
    20941 agccagagcg ttaatatcag tcagggaagt tccgctggtg gtcaggatat cctgcaccat
    21001 cggtaagatt cgttgagtat gttcacgagg gcaaagctca aaatgagcgt tgacagtacc
    21061 gtcgttccac agggcgacag agcaggcctc tgtcgcggta tcgatagcca gaattcgcat
    21121 gggtcttcgt gcttagatca ataaaaaggc gcgcatcata ccatactccg taacaaatta
    21181 cctggaggat ggtatcgcaa ggaaacgaac cgcacgggca atgtcacggg tgcgcggcgc
    21241 tggcggcaga ctggcgagaa acgtcgcgcc gtaagggcgc atcaccagcc gattatcgca
    21301 aatcaccagc acgccgcgat cgtcggcgtc gcgaatcagt cgccctactc cctgcttgag
    21361 agtaatcacc gcatccggca gttgtacttc atcgaacggg tcaccaccac gcaaacggca
    21421 atcttccatg cgcgctttta atagtggatc atccggtgag gtaaacggca acttgtcgat
    21481 aatcaccaat gacaatgtat cgccacgcac atccacccct tcccagaagc tgctggttgc
    21541 cacaagaagc gcgttaccgg cgctgacaaa ttgctgcaac agttgccctt tgctggtttc
    21601 cccctgcaac aaaacgggaa gcgtcatggt agcgcggaac tgctcagcca gatcgcgcat
    21661 catggcgtgc gaggtacaaa gcataaaaca acgaccgttg ttagcttcga tgatcggtcg
    21721 caacattgcc gccagttgcc gtgcggagcc cggttgattg gtttgcggca ggttgcgcgg
    21781 cacacagagt aacgcctggc ggctgtaatc aaacgggctg ggtaacagca acgactctgc
    21841 ctgttcaatg ccaagccgcg aggtaaaatg atgcagatcg tcgttcaccg acagcgttgc
    21901 tgaggtaaag atccagctac cgggtttttg cgccattaac tctttgaatt tatccgccac
    21961 gctgagcggc gtgagcgcca gagtaaaatg gcgcgaagtg cattcgtacc agtagctgta
    22021 gcccggctga ttgatctctt ttagccgttt cagccgcgta cgatacaacg tggcgcgctc
    22081 aaatgccgca tccagcaagg cggaacgccc cagcgacagt ttcgccacgt cataacaaag
    22141 ttccagggtg tcatcgagca gtaaaaacgc ccgttgaatt tgcggattag ctaacagttc
    22201 gcgcaggttg ccacgataac caggctcacc gagttgcaga cgaaaatcct gcgcgctctg
    22261 ggcaaggcgg tcggcgcact tttgtaactg ctgagtatct tttaattcgg tgcgataggc
    22321 gatggtgatg tcttttgcca ggtcgagtag ttgtcgactg gagagtgact gaccaaaata
    22381 ctggctggca atgtcaggca gctggtgggc ttcgtcgaag atcatgacgt cagcttccgg
    22441 gatcagttcg ccaaatccac tctctttcac caccatatcc gccagaaaga gatgatggtt
    22501 taccaccacc acatcggcgt ccatcgcttt tttgcgtgct ttgaccacaa agcaatcttt
    22561 atacatcggg cagtcgctgc caaggcagtt atcgttggtg ctggtgacca acggccacgc
    22621 ctgtgaatct tccgccacgc tgacgcaggt gctgatatca ccatcgactg tttgattaga
    22681 ccaggagcgc agcaggatca catcgcttaa gatttgtacc ggcagatcgc cccccgccag
    22741 cgcctgctgt tcgagacgtt cgaggcagag gtagtttgag cgccctttca gcaacgccac
    22801 gttgcccgta tatttcaacg cctttgaaac cgttggcaaa tcgcggctgt agagctgatc
    22861 ctgcaacgct tttgagccgg tcgagataat gactttcttt ttcgcccgca gcgcaggggc
    22921 caggtaagcg taggttttgc ccgtaccggt tccggcttcc accaccagcg gctggccttt
    22981 ttctatcgcc tgggtgacgg ctaccgccat ctgtcgctgt ggttctcgcg gcttaaagcc
    23041 tggtatcgct ttcgccagct gaccgtctgg tgcaaaatcg tccgtcacac taccccctgt
    23101 tgatttgaac agggattatg tcaggatgag ggcgctttcg ccagttgaag tggtgacggc
    23161 gacctcacat tgtggcagtc tttgcagcac gaaaatggaa gtataatgag gacaaaatga
    23221 tgactatcgt tcgtatcgat gctgaagccc gctggtctga tgtagtaatc cacaacaaca
    23281 cgctctacta cactggtgta ccggaaaacc tcgacgccga tgcctttgag caaaccgcca
    23341 acacgctggc gcagattgac gccgtgctgg aaaaacaggg cagcaataaa tcgagcattc
    23401 tggatgccac cattttcctg gccgataaaa acgacttcgc ggcgatgaat aaagcgtggg
    23461 atgcctgggt tgtcgcgggt catgcgccgg tgcgctgcac ggtacaagcg ggtttgatga
    23521 acccgaagta taaagttgaa attaagattg tggctgcggt gtaagcttta tcgaagcaaa
    23581 ataagtcaga cgataattta tcgacaatac tggtcggttt tgcataaatc gaccagagat
    23641 taagatatta cagttgatgc tcctgctaat tcattattta tttttataat ttcaatttta
    23701 tctataaaac taactatata aataaacata atcatgccgt tatgttcgcc cttcgattag
    23761 catctctggt tttctttctt acctgaacaa tctggttata gcgtgaggac tgaccattaa
    23821 ttttctccat cttactggat aacacactat gagctgctac ctttaaaaca cctgaatgtc
    23881 aacaggttaa ctcgtgccat atcgttataa tccctttgat attagataca aattaaggtc
    23941 atatatagcc ttatcgataa catggttaat tttaaggata agaatatgcc tgctgtaata
    24001 gataaagccc tggatttcat tggtgccatg gatgtatcag cgccaacacc aagttcgatg
    24061 aatgaaagta cggcgaaggg aatctttaaa tatttaaaag aactgggagt acccgcgagt
    24121 gccgctgata ttacggcgcg agctgaccag gaaggctgga accctggttt cacggaaaaa
    24181 atggttggat gggcaaaaaa aatggagtct ggtgaacgct ctgtgattaa aaatcctgaa
    24241 tacttttcaa catatatgca ggaagaacta aaagcactgg tttgagttaa atttacatca
    24301 gcataaatgg gtcaggacgc ttttaatcac atataaaaag cgtccttttt ctcacaatca
    24361 aataatcatc ggcttctata acgaaagcga tacggattag tgtgttactc gtcttcatcc
    24421 tcaaaacgcg ccacgattcg ctcgccggca tggttggcac gcagctcttc tgccaccaac
    24481 gcgattgcct ggccgctgct catcccttga gccatcagtt cctggatccg ctcgacagct
    24541 ttttgctgct gttcatgggt gagtgaaggt aaacctgcaa acattgttaa ctcctgctaa
    24601 attgttggcg ctaattattt catgctaccc ggcacatagc cagtagagtc aggactgatg
    24661 aagacgttat ctcccgctgt gattacttta ccctggcgtc aggacgccgc tgaattttat
    24721 ttctcacgct taagccacct gccgtgggcg atgcttttac actccggcta tgctgatcat
    24781 ccatatagcc gctttgatat tgtggtcgcc gagccgattt gcactttaac cactttcggt
    24841 aaagaaaccg ttgttagtga aaacgaaaaa cgcacaacga ccactgatga cccgctacag
    24901 gtgctccagc aggtgctgga tcgcgcagac attcgcccag cgcataacga agatttgcca
    24961 tttcagggcg gcgcgctggg gttgtttggc tacgatctgg gccgccgttt tgagtcactg
    25021 ccagaaattg cgcagcaaga tatcgttctg ccggatatgg cagtgggtat ctacgactgg
    25081 gcgctggttg ttgaccacca gcgtcaaaca gtttctttgc tgagtcataa tgatgtcaat
    25141 gctcgtcggg cctggctgga aagccagcaa ttctcgccgc aggaaggttt cacgctcact
    25201 tccgactggc aatccaatat gacccgcgag cagtacggcg aaaaatttcg ccaggtacag
    25261 gaatatctgc acagcggtga ttgctatcag gtgaatctcg cccagcgttt tcatgcgacc
    25321 tattctggcg atgaatggca ggcattcctt cagcttaatc aggccaaccg cgcgccattt
    25381 agcgcttttt tacgtcttga acagggtgca attttaagcc tttcgccaga gcggtttatt
    25441 ctttgtgata atagtgaaat ccagacccgc ccgattaaag gcacgctacc acgcctgccc
    25501 gatcctcagg aagatagcaa acaagcagaa aaactggcga actcagcgaa agatcgtgcc
    25561 gaaaatctga tgattgtcga tttaatgcgt aatgatatcg gtcgcgttgc cgtagccggt
    25621 tcggtaaaag taccagagct gttcgtggtc gaacccttcc ctgccgtgca tcatctggtc
    25681 agcaccataa cggcgcggct accagaacag ttacacacca gcgatctgct gcgcgcagct
    25741 tttcctggtg gctcaataac tggggctccg aaagtacggg ctatggaaat tatcgacgaa
    25801 ctggaaccgc accgacgcaa tgcctggtgc ggcagcattg gctatttgag cttttgcggc
    25861 aacatggata ccagcattac tatccgcacg ctaactgcca ttaacggaca aatatactgc
    25921 tctgcgggcg gtggaattgt cgccgatagc caggaagaag cggaatatca ggaaactttt
    25981 gataaagtta ataagatcct acgtcaactg gagaaataaa cgtggaacac cgtagcctga
    26041 cgcttgatga ttttttatcg cgctttcaac ttttgcgtcc gcaaatgaac cgggaaacgt
    26101 taaatcatcg tcaggctgcc gtgttaatcc ccatcgtccg tcgaccgcaa ccggggttgt
    26161 tgctgactca gcgttcgatt catctgcgta aacacgctgg acaagtggca ttccctggag
    26221 gcgcagtcga tgacacggac gcatcagtta tcgccgccgc gctgcgcgaa gctgaagaag
    26281 aggtcgctat accgccttcc gccgttgaag ttatcggcgt gctgccgccc gtcgatagcg
    26341 taactggcta ccaggtaacc ccagtggtcg gcattatccc gcccgatctg ccgtatcgcg
    26401 ccagtgaaga tgaagtctcg gcggtgtttg aaatgccgct cgcccaggca ttacatctgg
    26461 gtcgttatca tcctttagat atctaccgcc gtggcgattc acatcgggta tggctgtcct
    26521 ggtacgaaca gtattttgta tggggaatga ccgcaggcat aattcgtgag ctggcgctgc
    26581 aaattggtgt gaaaccctga ctatacttat ctttacatct acaaaacact acttgagaca
    26641 atcatcgcaa tattagttaa atcgcggttt ttgattagtt taattcatgt gaatagttaa
    26701 gccagtcgcc gcgttccctc ttacactatg cgctgttatt agttcgttac tggaagtcca
    26761 gtcaccttgt caggagtatt atcgtgatta gtctattcga catgtttaag gtggggattg
    26821 gtccctcatc ttcccatacc gtagggccta tgaaggcggg taaacagttc gtcgatgatc
    26881 tggtcgaaaa aggcttactg gatagcgtta ctcgcgttgc cgtggacgtt tatggttcac
    26941 tgtcgctgac gggtaaaggc caccacaccg atatcgccat tattatgggt cttgcaggta
    27001 acgaacctgc caccgtggat atcgacagta ttcccggttt tattcgcgac gtagaagagc
    27061 gcgaacgtct gctgctggca cagggacggc atgaagtgga tttcccgcgc gacaacggga
    27121 tgcgttttca taacggtaac cttccgctgc atgaaaacgg tatgcaaatc cacgcctata
    27181 acggcgatga agtcgtctac agcaaaactt attattccat cggcggcggt tttatcgtcg
    27241 atgaagaaca ctttggtcag gatgctgcca acgaagtgag cgtgccgtat ccgttcaaat
    27301 ctgccaccga actgctcgcg tactgtaatg aaaccggcta ttcgctttct ggtctcgcta
    27361 tgcaaaacga actggcgctg cacagcaaga aagagatcga cgagtatttc gcgcatgtct
    27421 ggcaaaccat gcaggcatgt atcgatcgcg ggatgaatac cgaaggcgta ctgccaggcc
    27481 cgttgcgcgt gccacgtcgt gcgtcggccc tgcgccggat gctggtttcc agcgataaac
    27541 tgtctaacga tccgatgaat gtcattgact gggtaaacat gtttgcgctg gcagttaacg
    27601 aagaaaacgc cgccggtggc cgtgtggtaa ctgcgccaac taacggtgcc tgcggtatcg
    27661 ttccggcagt gctggcttac tatgaccact ttattgaatc agttagccca gacatctata
    27721 cccgttactt tatggcagcg ggcgcgattg gtgcactgta taaaatgaac gcgtctattt
    27781 ccggtgcgga agtcggttgc cagggcgaag taggtgttgc ctgttcaatg gctgctgcgg
    27841 gccttgccga gctgttgggc ggtagcccgg aacaggtttg tgtggcagcg gaaattggca
    27901 tggagcacaa cctcggtctg acctgcgacc ccgttgcagg gcaagttcag gtgccgtgca
    27961 ttgaacgtaa tgccattgcc tctgtgaagg cgattaacgc cgcacggatg gctctgcgcc
    28021 gcaccagtgc accgcgcgtc tcgctggata aggtcatcga aacgatgtac gaaaccggta
    28081 aggacatgaa cgctaaatac cgcgaaacct cacgcggtgg tctggcaatc aaagtccagt
    28141 gtgactaata cttcttactc gcccatctgc aacggatggg cgaatttatt cccgctttct
    28201 cgtctgctgt aattttcccc actacacttc cactgttgcg tcaggcgttt gtcgccatac
    28261 gcttacaggg tggcccgcat gcaaaaagca caacggatca ttaaaaccta tcgccgtaat
    28321 cgaatgattg tttgtacgat ttgcgcactc gttacgctcg cttcgaccct gagcgtgcga
    28381 tttatttcac agcgtaactt aaatcaacaa cgggtagtac aattcgccaa tcacgctgta
    28441 gaggaattag ataaagtact gcttccccta caggcaggta gcgaagtctt gcttccgctg
    28501 attggtctgc cctgctctgt cgcccatttg ccattacgta aacaggcggc aaaactccaa
    28561 actgtgcgat ccattggcct ggtgcaagac ggcacacttt attgctccag catttttggt
    28621 tatcgcaatg tgcccgtcgt ggacattctg gctgaacttc ctgcaccgca accactttta
    28681 cgcctgacga ccgaccatgc cctgattaaa ggcagtccgg ttttgattca atggacgcca
    28741 gcagcgggca gtagcaaagc tggggtcatg gagatgatta acatcgactt actggcggca
    28801 atgctgcttg agccacaact gccgcaaatc agtagcgcca gcctgacggt ggacgatcgg
    28861 catttgctct atggtaatgg gctggtagat tcccttccgc aacctgaaaa caatgaaaac
    28921 tatcaggttt cttcgcaacg ctttcctttt accattaacg ttaatggtcc gggggctacg
    28981 gcgctggcat ggcactatct tccaacacaa ttaccgctgg cggtgctgct aagtttactt
    29041 gtgggctata tcgcctggct ggcgaccgct taccggatga gtttttcccg cgaaatcaat
    29101 ctgggcctgg cgcaacatga gttcgaattg ttctgccagc ctttgcttaa tgcgcgcagc
    29161 cagcaatgta ttggcgtaga gattttgctg cgctggaaca atccgcgtca gggctggatt
    29221 tcgccggatg tgtttattcc tatcgcggaa gaacatcatt taattgtgcc actgacccgc
    29281 tatgtgatgg cagaaaccat tcgtcagcgc catgttttcc cgatgagtag tcagtttcat
    29341 gttggcatta acgtcgcacc cagccatttt cgccgtggtg tgctgataaa agatctcaat
    29401 cagtactggt ttagcgctca cccgattcag caactgatcc tcgaaatcac cgaacgcgat
    29461 gccttactgg atgttgatta tcggattgcc cgcgagctac atcgtaaaaa cgtcaaactg
    29521 gcgattgatg acttcggcac cggcaatagt tcattttcct ggcttgaaac attacgtcct
    29581 gacgtgctga aaattgataa gtcatttacc gcagctatag gttctgacgc ggttaactcg
    29641 acggtgaccg atatcatcat cgcactgggg caaagactga atattgaact ggtggcggag
    29701 ggtgtggaaa cgcaagaaca ggcgaaatat ttgctccgtc atggcgtcca tattttgcaa
    29761 gggtatttgt acgcacagcc gatgccacta cgtgattttc ccaaatggct ggcgggcagc
    29821 caaccgccgc ccgcccggca taatggacat atcacaccca ttatgccgtt acgttaacgc
    29881 ttactcatct tcatcgtgcg ccggttgctc tttaacaatg cgaaccagat caacacgata
    29941 atcattggct tcaatgatgg tgatatgcag tggccctaca tcaatcacat cgcccacacg
    30001 gggaatgtga ccatttgccg agatcacgag gcccgcgacc gtcgcgatat catcgtcatc
    30061 ggcaaggtgc tcaacatcaa gcgcctgctg caaggcatgc aaatctgtac cgccttttac
    30121 cagccagccg tcaccgtcgg taatgatttc cggcgtttcg tcagcgtccg ggaattcacc
    30181 cgcaatggct tccagcacat ccagcggcgt gaccaagcct tgtaccacac caaactcgtt
    30241 ggtcacgata acaaagctcc cgcgagcacg acgcagcacg cccagcaggt tgatcggatc
    30301 gagggtttcc gggacgataa tcgccggaga cgccgaagca atcgccgcca catcaacgcc
    30361 ctcttccagc gccaccagca gttctttagc acgtacgatt ccgatgattt catccagttc
    30421 accgcgacat accgggaaca ggctgtgcgg tgaggagagc agctgctcgc ggatttcatc
    30481 taccccgaga ttagcgtcaa cccagcttat ttcaccgcgc ggcgtcatga tcccgcgcag
    30541 agaacgcgac gccagcgtca gtacgccgtt aatcatgtaa cgttcttctt cggcaaatgc
    30601 accttccggg atcggcatcg gcatcgggtt atcggcatcg tgctgaacat tggcctgacg
    30661 tttcccgccc atcaaacgca ggatggcatc ggcagtacgc gctcgcagcg gcaaagtcga
    30721 ctggtgacga ataaagttgc gacgcgcaat ctggttaaac acttcgatga tgatcgagaa
    30781 gccaatcgcg gcatacaggt aacctttcgg aatgtggaaa ccgaaacctt ctgccaccag
    30841 actcagacca atcattaaca ggaagctcag acagagcacc accaccgtgg ggtgctggtt
    30901 aacaaatcgc gtcagcggtt tcgatgccag cagcataact gccatcgcaa tcactaccgc
    30961 cgccatcatc accggcaaat ggttaaccat ccctactgca gtaattaccg catccaacga
    31021 gaagacggcg tcaaggatga caatctgtgt gacgaccacc cagaaactgg cgtagccttt
    31081 accgtggccg gaatcatgat cgcggttttc cagccgttca tgcagttcgg ttgttgcttt
    31141 gaacagcaag aatatgcccc cgaacaacat aatcaggtcg cgtccggaga aggagaaatc
    31201 catgacggta aatagcggtt tggtcagcgt gaccatccat gaaatcagcg acagcagccc
    31261 cagacgcata atcagcgcca gtgataatcc cagcaaacgc gctttatcgc gttgttttgg
    31321 cggcagtttg tcagcaagaa tggcaatgaa gaccaggtta tcgataccca gcacaatttc
    31381 gagaacaaca agcgtgagta gccccgccca aattgagggg tccattaaga attccatgac
    31441 aagctcctgc ttaaggaata gctattcgac gccagaaata atgcaggcgt aacgacaaaa
    31501 tgcaaacgaa aggtgcggca tagagtgcca gaaaggcagg cgttaaaagg cctgatgctg
    31561 aaatgacgtc ggtgacgatc catactgcgg gctactgccc tatactccat ggttgttaaa
    31621 cgggagttaa acatatcaga gacgcctctg atttggcaaa gatttacctt cctttgcaaa
    31681 cgaatgtgac aaggatattt tacctttcga aatttctgct aa
//
LOCUS       DAZKGF010000034        31141 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZKGF010000034 DAZKGF010000000
VERSION     DAZKGF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.034"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1771
                     /locus_tag="ACHOQV_001564"
     CDS             <1..1771
                     /locus_tag="ACHOQV_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HFP9629959.1"
                     /translation="VAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIVDPYGNAPLT
                     ALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQKFANKVTVE
                     WKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLYLVNTHTFTA
                     QGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLDQDTFYDGRD
                     RDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFADATHESIETP
                     NGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDPKRDALLGAL
                     DAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAKDDSIILSSR
                     HQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGLCENSDFDFT
                     YTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGTVQQVWEYGK
                     ERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYKTKEVKVEID
                     VLSDKPNQTHYRALLVRPQQMFK"
     gene            1789..2457
                     /locus_tag="ACHOQV_001565"
     CDS             1789..2457
                     /locus_tag="ACHOQV_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFP9629960.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSLA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2472..3143
                     /gene="dsbI"
                     /locus_tag="ACHOQV_001566"
     CDS             2472..3143
                     /gene="dsbI"
                     /locus_tag="ACHOQV_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HFP9629961.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIVRG"
     gene            complement(3189..3977)
                     /gene="ygiD"
                     /locus_tag="ACHOQV_001567"
     CDS             complement(3189..3977)
                     /gene="ygiD"
                     /locus_tag="ACHOQV_001567"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFP9629962.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4120..4893
                     /gene="zupT"
                     /locus_tag="ACHOQV_001568"
     CDS             4120..4893
                     /gene="zupT"
                     /locus_tag="ACHOQV_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFP9629963.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(4951..5121)
                     /gene="yqiD"
                     /locus_tag="ACHOQV_001569"
     CDS             complement(4951..5121)
                     /gene="yqiD"
                     /locus_tag="ACHOQV_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFP9629964.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(5383..6036)
                     /gene="ribB"
                     /locus_tag="ACHOQV_001570"
     CDS             complement(5383..6036)
                     /gene="ribB"
                     /locus_tag="ACHOQV_001570"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFP9629965.1"
                     /translation="MNQTLLSSFGTPFERVENALVALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(6140..6288)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6410..6709
                     /gene="ubiK"
                     /locus_tag="ACHOQV_001571"
     CDS             6410..6709
                     /gene="ubiK"
                     /locus_tag="ACHOQV_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFP9629966.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(6744..6944)
                     /gene="glgS"
                     /locus_tag="ACHOQV_001572"
     CDS             complement(6744..6944)
                     /gene="glgS"
                     /locus_tag="ACHOQV_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFP9629967.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            7213..7842
                     /gene="yqiJ"
                     /locus_tag="ACHOQV_001573"
     CDS             7213..7842
                     /gene="yqiJ"
                     /locus_tag="ACHOQV_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP9629968.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCLMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            7869..9530
                     /gene="yqiK"
                     /locus_tag="ACHOQV_001574"
     CDS             7869..9530
                     /gene="yqiK"
                     /locus_tag="ACHOQV_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP9629969.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            9770..9829
                     /locus_tag="ACHOQV_001575"
     CDS             9770..9829
                     /locus_tag="ACHOQV_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9629970.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(9949..11382)
                     /gene="hldE"
                     /locus_tag="ACHOQV_001576"
     CDS             complement(9949..11382)
                     /gene="hldE"
                     /locus_tag="ACHOQV_001576"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFP9629971.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNQ
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(11430..14270)
                     /gene="glnE"
                     /locus_tag="ACHOQV_001577"
     CDS             complement(11430..14270)
                     /gene="glnE"
                     /locus_tag="ACHOQV_001577"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFP9629972.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFLQD
                     SIIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPFDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(14293..15594)
                     /gene="ygiF"
                     /locus_tag="ACHOQV_001578"
     CDS             complement(14293..15594)
                     /gene="ygiF"
                     /locus_tag="ACHOQV_001578"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFP9629973.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            15836..16456
                     /gene="ygiM"
                     /locus_tag="ACHOQV_001579"
     CDS             15836..16456
                     /gene="ygiM"
                     /locus_tag="ACHOQV_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFP9629974.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            16520..17758
                     /gene="cca"
                     /locus_tag="ACHOQV_001580"
     CDS             16520..17758
                     /gene="cca"
                     /locus_tag="ACHOQV_001580"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFP9629975.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVAGWKEQRCPKPE"
     gene            complement(17946..18767)
                     /gene="bacA"
                     /locus_tag="ACHOQV_001581"
     CDS             complement(17946..18767)
                     /gene="bacA"
                     /locus_tag="ACHOQV_001581"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9629976.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(18858..19226)
                     /gene="folB"
                     /locus_tag="ACHOQV_001582"
     CDS             complement(18858..19226)
                     /gene="folB"
                     /locus_tag="ACHOQV_001582"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFP9629977.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            19331..19948
                     /gene="plsY"
                     /locus_tag="ACHOQV_001583"
     CDS             19331..19948
                     /gene="plsY"
                     /locus_tag="ACHOQV_001583"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP9629978.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(19961..20893)
                     /gene="ttdR"
                     /locus_tag="ACHOQV_001584"
                     /gene_synonym="dan"
     CDS             complement(19961..20893)
                     /gene="ttdR"
                     /locus_tag="ACHOQV_001584"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFP9629979.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            21100..22011
                     /gene="ttdA"
                     /locus_tag="ACHOQV_001585"
     CDS             21100..22011
                     /gene="ttdA"
                     /locus_tag="ACHOQV_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFP9629980.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            22008..22613
                     /gene="ttdB"
                     /locus_tag="ACHOQV_001586"
     CDS             22008..22613
                     /gene="ttdB"
                     /locus_tag="ACHOQV_001586"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFP9629981.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            22662..24125
                     /gene="ttdT"
                     /locus_tag="ACHOQV_001587"
     CDS             22662..24125
                     /gene="ttdT"
                     /locus_tag="ACHOQV_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFP9629982.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(24168..25181)
                     /gene="tsaD"
                     /locus_tag="ACHOQV_001588"
     CDS             complement(24168..25181)
                     /gene="tsaD"
                     /locus_tag="ACHOQV_001588"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP9629983.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            25419..25634
                     /gene="rpsU"
                     /locus_tag="ACHOQV_001589"
     CDS             25419..25634
                     /gene="rpsU"
                     /locus_tag="ACHOQV_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP9629984.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            25745..27490
                     /gene="dnaG"
                     /locus_tag="ACHOQV_001590"
     CDS             25745..27490
                     /gene="dnaG"
                     /locus_tag="ACHOQV_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9629985.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKQAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDNQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            27685..29526
                     /gene="rpoD"
                     /locus_tag="ACHOQV_001591"
     CDS             27685..29526
                     /gene="rpoD"
                     /locus_tag="ACHOQV_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP9629986.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(29604..30110)
                     /gene="mug"
                     /locus_tag="ACHOQV_001592"
     CDS             complement(29604..30110)
                     /gene="mug"
                     /locus_tag="ACHOQV_001592"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFP9629987.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            30235..30310
                     /locus_tag="ACHOQV_001593"
     tRNA            30235..30310
                     /locus_tag="ACHOQV_001593"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30268..30270,aa:Ile,seq:cat)
     gene            complement(30364..31128)
                     /gene="nfeF"
                     /locus_tag="ACHOQV_001594"
                     /gene_synonym="yqjH"
     CDS             complement(30364..31128)
                     /gene="nfeF"
                     /locus_tag="ACHOQV_001594"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFP9629988.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISVGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQSDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEGGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
BASE COUNT         7680 a         8357 c         7801 g         7303 t
ORIGIN      
        1 cgtagccgga actgtggcga taaccctggg tttgtcagca tcgggggtga tggctgcggg
       61 ttttaaacca gcgccgcctg ccgggcaact gggtgcggtc attgtcgatc cctacggcaa
      121 tgcaccactg accgctttgg ttgacttaga tagccatgtt atttctgacg tcaaagttac
      181 cgtccatggg aagggcgaaa aaggtgtaga aatcagctat cccgtgggtc aggaatcact
      241 aaaaacttac gatggtgtac cgatttttgg tctttatcag aaatttgcta acaaagtgac
      301 cgttgagtgg aaagaaaacg gcaaggtcat gaaagatgat tatgtggtgc acacttcggc
      361 catcgtcaat aattacatgg ataaccgctc tatatccgat ttacaacaga ccaaagttat
      421 taaagtcgca ccgggttttg aagatcgcct ctatctggtt aatacccaca cctttaccgc
      481 ccaaggttcc gatctccact ggcatggtga gaaagataaa aatgccggta tccttgatgc
      541 gggtccggca accggcgcac taccttttga tatcgcgccc tttactttca tcgtcgatac
      601 cgaaggtgaa taccgctggt ggctggatca agacactttc tacgatggtc gtgaccgcga
      661 tatcaacaag cgtggttatc tgatgggtat ccgcgaaacg ccacgcggca cctttaccgc
      721 tgtacaaggt cagcactggt acgagttcga catgatgggg caggtgctcg aagatcacaa
      781 actaccgcgc ggatttgctg acgccactca tgagtccatt gagacgccaa atggcacggt
      841 actgttgcgc gtaggtaaga gtaactatcg tcgcgatgac ggcgtacacg tcaccaccat
      901 tcgtgaccat atcctcgaag ttgataaatc tggtcgcgtt gtcgatgtat gggatctgac
      961 caagatcctc gatccgaaac gcgatgcact gctcggcgcg ctggatgcag gtgcagtttg
     1021 cgttaacgtt gaccttgccc atgcaggaca acaggcaaaa ctggaaccag atacaccgtt
     1081 cggcgacgcg ctgggtgtag ggccaggccg caactgggcg cacgtgaact ctatcgccta
     1141 tgacgcaaaa gatgactcta tcatcctctc ctctcgccac cagggtgttg tgaaaattgg
     1201 tcgtgataag caagtgaaat ggatccttgc gccctctaaa ggttgggaaa aaccgctggc
     1261 cagcaagctg ctgaaaccgg ttgatgctaa cggtaagcca attacctgta acgaaaatgg
     1321 cctgtgcgaa aactcagact tcgactttac ctacacccag cataccgcct ggatttccag
     1381 caaaggaacg ctcaccattt ttgataatgg cgatgggcgt catctggaac aacctgcctt
     1441 accaaccatg aaatactccc gctttgtgga atataagatt gatgagaaga aaggcaccgt
     1501 tcagcaagtg tgggaatacg gtaaagaacg tggctacgat ttctatagcc cgattacctc
     1561 catcattgaa tatcaagccg accgtaacac catgtttggc ttcggtggtt ctattcattt
     1621 gttcgatgtc gggcagccaa ccgtcggtaa gttgaacgaa atcgattaca aaaccaaaga
     1681 agtgaaagtg gaaatcgacg tgctgtcaga taaacccaat cagactcact atcgtgcact
     1741 gttagtccgt ccacaacaga tgttcaaata attaagaagg gaataaaaat gtcgaaatta
     1801 gggatctcat cactatttaa aaccatactg cttaccgcag ctttggctgt ttcatttacc
     1861 gcttctgcat ttaccgaagg cacggattac atggttctgg aaaaacctat tccgaatgcc
     1921 gacaaaacgc tgattaaggt attcagctac gcctgcccgt tctgttacaa gtacgacaaa
     1981 gcggttaccg gtccggtatc ggaaaaagtg aaagatatcg ttgctttcac gccgttccat
     2041 ctggaaacca aaggtgaata cggcaaacaa gcaagcgaag tctttgcggt cttgatcaat
     2101 aaagataaag cagcaggcat ttccttattt gatgctaact ctcagtttaa gaaagccaag
     2161 tttgcctact acgcggctta tcacgacaaa aaagaacgct ggtctgacgg taaagatccg
     2221 gctgctttta tcaaaaccgg tctggatgcc gcaggtatga gcttggcgga ttttgaagct
     2281 gcactgaaag aaccagcggt tcaagaaaca ctggaaaaat ggaaagcctc ttatgatgtc
     2341 gccaaaatcc agggggttcc agcctatgtc gttaatggta agtacttgat ctacaccaag
     2401 agcatcaaat ccatcgacgc gatggcagac cttatccgtg agttagcaag caaataacag
     2461 ggaaacaggc tatgggtatt aagggaatgt ggaaagacct tcgcacatca ccagttgaca
     2521 cactggtcag atggcaggag caacgcttgt tgtggttgtt aatggccgtt gcgatgggcg
     2581 cgttaattat cctggcgcac tctttcttcc agatctatct ctacatggca ccctgtgagc
     2641 aatgtgtata cattcgctac gccatgtttg tgatggttat tggaggattg gttgcagcga
     2701 tcaacccaaa gaacatcatc ctaaagctaa ttggctgcgt gatggcattt tacggcagca
     2761 ttttgggact gaagttttcg ctcaaactaa acgatatcca ccatgcagta cataatccgg
     2821 atcccgattc actgtttggc gtacagggtt gctctaccga tccaactttt ccctttaacc
     2881 tgccgctggc acagtgggca ccaaattggt tcaagcctac aggggattgt ggctatgacg
     2941 caccaatcgt tcctgatggc gtcacactga gcagcacgca acagtggttt gtggaaatgt
     3001 atcagcaatc tgaaggctgg tatctgctac caccatggca ttttatgaat atggctcagg
     3061 cttgtatgct ggcttttggt atgtgtctgg tgctgctggt cattatgagt ggcgcatggg
     3121 cgcttaaaat cgtcagaggc taactcagca acacccgaag ccagtcgttc tggcttcggg
     3181 ttcttttatt agcctatctg caccgacaac atacttaaac tgcccatttc tataccatcg
     3241 accggaatgg taattggctc ctgcccatcc cacgcaccta agacatacaa caacggcaga
     3301 tagtgctctg gcgttgggtt cgataacgcg ccaccttcat ggtcgagata attcaccaga
     3361 ggatgctgct ctaccggccc ttgccacgtc agattcgctt tcacatactc attaaacgac
     3421 gtcgcccacg gatacggtga actatcaccg tgccacttca ctgtgcgcag gttatgcacc
     3481 acgttaccgc tggcgaccaa cattattcct tcatctcgca gcgccgcgag tttgcgcccc
     3541 atttcgaaat gccaggcggc aggtttgcta ctgtcgatac tcaactgcac catcgggata
     3601 tccgcgtcag gatacatctt aatcagcacg ccccaggaac cgtggtcaaa gccccaggct
     3661 tctttatcca gcgtcaccgg gatcggcgct aacagctcaa ccagacgctg tgccagcgca
     3721 ggcgaacccg gagcaggata atgcgtatcg tacagcgcct gcgggaagcc accaaagtca
     3781 tgaatcgtgg gcggcgtctc catcgcggtc actcctgttc cacgggtaaa ccagtgagcc
     3841 gaaaccacca caatcgcttg cgggcgtggc aatgtcatcc ccaacgtctg ccagctgcgg
     3901 gtatacaaat tatcttccag cacgttcatc ggactaccgt gacctaaaaa caatgctggc
     3961 atacgtgttg aagacatgat gatatcctta actaaaggtg tcattttgat atcctcacaa
     4021 tacgcttgtt cggcggagta agaacccgga taacaatgat gatgatcatc agttattttg
     4081 acgatctgcc tgaaggtgaa gatttataag gagttgtcga tgtcagtacc tctcattctg
     4141 accatactgg cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct cgggcaaaaa
     4201 ccctcgaacc gcttactggc gttttcgcta ggatttgcgg cggggatcat gttgctcatc
     4261 tcattaatgg aaatgcttcc tgccgcacta gccgccgaag gaatgtcgcc tgtgttgggt
     4321 tatggaatgt ttatcttcgg tctgcttggc tattttggcc tggaccgcat gttgccacat
     4381 gcgcatccgc aggatttaat gcaaaaatcg gtgcagccgt tgccaaaatc gatcaagcgc
     4441 acagccattc tgctcactct cggcatcagt ctgcataact tcccggaagg gattgccacc
     4501 tttgtcacgg cgagcagcaa cctggagctg ggctttggca tcgcactggc cgttgcgtta
     4561 cacaatattc ctgaaggtct ggcggttgca ggcccggttt acgcggcaac ggggtctaaa
     4621 cgcaccgcaa ttctttgggc ggggatttct ggactggcag aaattcttgg tggtgtgctg
     4681 gcgtggttaa tcctcggtag catgatttcc ccggtggtca tggcggcaat catggcggcg
     4741 gttgcaggaa ttatggtggc gctctcggtt gatgaattaa tgccgctcgc caaagagatt
     4801 gaccctaata ataaccccag ctatggcgta ctatgtggaa tgtcagtgat gggattcagt
     4861 ttagtgctgc tacaaacggc gggaattggt taaaagcaac atcagaagtt gtccagcgca
     4921 tggacaactt ctgccagctt cactaaagcc ttattctacc ttttcggctg tctcttctct
     4981 cgtactgttt aagtatttgt tccgcgcttc aagcagtgcg tctctgtcct ggcgaaacgc
     5041 ccgacaataa cgtttcaaat gcaggaaata acccaccaca acaagagcaa tcaatacaat
     5101 ccagtaccag gcgataaaca tccttttacc cttaatattt aacaatacta aacatttagc
     5161 gcataaattt cacatatcct ttttcggata tattcatatg gtcgcataat aacggaacaa
     5221 ttatatgatt ttgatcataa tgaaaaagaa tgcctgatat caatgaaatt ttctcataga
     5281 aagagaagcg gaattaacat cttgcatagc agggcaaaat aaaaaccgaa gccagctaca
     5341 acttcggttt cttgattaag acagtaaatt aagcagcgat tttcagctgg ctttgcgttc
     5401 atgcgcctga cgataagcca ccaggtcttc aatagtcacg agcgccatat tgtgtttatt
     5461 ggcaaactca atgcactctg gtgcacgcgc catcgtgcca tcgtcattag tcagctcaca
     5521 cagcacacca gccggtttaa agccagccag cgtcatcaga tcaatagtcg cttcagtatg
     5581 gccgccacgc gtcagtacac caccagcctg agcgcgaagt gggaaaacgt ggcccgggcg
     5641 attcagatca gaaggttttg cgccatcggc aatcgctgcg cgaacggtcg taatacggtc
     5701 agcggcagaa acaccggtag tcacaccttc agctgcttca atggtcacgg taaaaccggt
     5761 gccataggcg ctggtgttat tttctaccat cattggcaga tcgagttgtt tacggcgatc
     5821 ttcagtgatg cacaggcaaa caataccgct accgtggcga atggtcagcg ccatctgctc
     5881 aacggtcatg gtttctgccg ggaagatcat atcaccttcg ttttcacggt cttcatcatc
     5941 aagcaccatt acaccgcgtc cttcacgcag cgcaaccagt gcattttcaa cacgttcgaa
     6001 aggcgtacca aaagaggaaa gtagcgtctg attcatggta aaaaaacctc actaaaatta
     6061 tggttaccag aatcagggca gtcttaggag tggcggcata tagccaaaat aacgtgagcg
     6121 ggtccatgcc cgacagaatc gttactctct cccatccgga ctctaaccgt cggccccgga
     6181 attacaccgg atctgctgtc ctttgagttc gcacccaaag cgctcgcggg ctttcaactg
     6241 agttgattta ccgccggtgg ggaatttcgc cccgccctga gaataagcgg attcactata
     6301 acgctaatga ttagcggcag caacgcatag cttcacataa ttctggttta tgacttaccc
     6361 ttatcgcact acaatggcac tcaacaccta tcagtacagg gaaactacaa tgattgaccc
     6421 gaaaaaaatt gagcaaatcg ctcgccaggt tcacgaatca atgcctaaag gaatcaggga
     6481 gttcggggaa gatgtggaga aaaaaatccg ccaaacccta caagcgcagc tgacgcgtct
     6541 cgatctggta agccgcgaag aattcgacgt tcagacgcaa gtcctgttac ggactcgtga
     6601 aaaactggcg ttactggagc agcgcatcag tgaactggaa gcgcgcaata attcggtagc
     6661 agatcttcaa tctcctccgg ctatcccgcc tattgataaa gcagaataaa gcatcacaaa
     6721 aacgggccag acggcccgta atatcagtgt tctaattcaa gttctctggt ttgcatcatc
     6781 tgttggcaat accaggcata acgtgcgcaa aaccaggttc tatgttcttc atccatgtta
     6841 ccagtaacgg ccagaatatc gaccgggcga ccttctgcgt gcattctggc aaaactacgc
     6901 gccaggaaat cgaaattatt taaagaatta agactatgat ccataagcac atcctccggt
     6961 tatcgcatac cttttaaggc catatgcttt ttctgatttc agtattcccc aatcagtgta
     7021 gtgcctttaa acataacgtg cgtaaatata ttgtccccga tcacactttt tagtgaaaaa
     7081 taaaaaacca tattaaaaga ctttctcttc cagaacttct ctaacatgtg ttattattaa
     7141 caaagataaa tgtaagcaag gaaactatcc taaagtaaat attgcgtcac taaatggaca
     7201 tcggagtgac atatgatttt attcgccgac tataacaccc cttatctttt cgctatttcc
     7261 tttgtactgt tgatcggcct tttagaaatt tttgcgctta tctgcggtca catgctctcc
     7321 ggtgctcttg atgcacatct tgaccattat gattccataa ccacaggtca tattagccag
     7381 gcacttcatt atctcaatat cggaagattg ccagctctcg tcgttctctg cttactggct
     7441 ggtttttttg gtcttattgg catcctgttg cagcatacct gcctcatggt ctggcaatca
     7501 ccactctcga acctgtttgt ggttcctgtc agtttactgt ttacgattat tgcggtgcat
     7561 tataccggta aaatagttgc gccctggatt ccacgcgatc acagctcagc tattacagaa
     7621 gaagaatata ttggtagcat ggcgttaatt accggtcatc aagccacttc aggtaatccc
     7681 tgcgaaggaa aactcaccga tcaatttggc caaatacatt atttactact ggagcctgaa
     7741 gaaggaaaaa ttttcactaa aggagacaaa gtgttaatta tttgccgact ttctgcaact
     7801 cgatatttgg cggaaaacaa tccctggccc caaatattgt aatttattaa tcaaaaggaa
     7861 atttaataat ggatgatatt gttaattctg tgccctcctg gatgtttacc gcgattattg
     7921 ccgtatgcat tctgtttatt attggaatta ttttcgccag gctctatcgt cgcgcttcgg
     7981 cagagcaagc ttttgttcgt actggtttag gtgggcaaaa agtggtaatg agcggtggcg
     8041 caatcgtgat gccgatcttt catgaaataa tccccatcaa tatgaatact ctgaagctgg
     8101 aagtcagccg ctcaaccatt gatagcctga ttacgaaaga tcgtatgcgc gtcgatgtcg
     8161 ttgtcgcttt ctttgtgcgg gtaaaaccgt cagtggaagg tattgccacc gccgcccaga
     8221 cgcttgggca acgcaccctg tcgcctgaag acttacgtat gttggttgaa gataaatttg
     8281 tcgatgccct ccgtgcaaca gctgcacaaa tgaccatgca tgagttacag gatacccgcg
     8341 agaacttcgt gcagggcgta caaaataccg tagctgaaga tctgtcgaaa aacggcctgg
     8401 aactggaaag cgtttcactt accaacttta accagacgtc gaaagaacat ttcaatccta
     8461 acaacgcctt tgacgccgaa ggtttaacta agctgacgca ggagacggag cgccgtcgtc
     8521 gcgaacgtaa cgaagttgaa caggatgtag aagttgcggt gcgtgagaaa aaccgtgatg
     8581 cgctttcgcg gaagctggag attgaacagc aagaagcgtt tatgacgctt gagcaggagc
     8641 agcaggttaa aacccgtacc gctgagcaga atgcgaaaat tgcggctttt gaggccgagc
     8701 gtcgtcggga agcagagcag acgcgaattc tggctgaacg acagattcag gaaacggaaa
     8761 tcgaccgcga acaggccgtc cgttccagaa aagttgaagc tgaacgtgaa gttcgcatta
     8821 aagagatcga acagcagcag gtcaccgaaa tcgccaacca gacgaaatcg atcgcgattg
     8881 ccgccaaatc ggaacagcag tcacaagcgg aagcgcgtgc caaccttgca cttgcagaag
     8941 cggtaagcgc ccagcaaaac gtagaaacca ctcgccagac tgccgaagcc gatcgtgcta
     9001 aacaagtggc gttgatcgcc gcagcgcagg atgcagaaac caaagcggtt gaactgaccg
     9061 tgcgggcgaa agcagaaaaa gaagccgcag aaatgcaggc ggcggctatc gttgagttag
     9121 ccgaagctac acgtaaaaag ggtctggcgg aagcagaagc acaacgtgcg ctgaacgatg
     9181 ctatcaacgt actttctgat gaacaaacca gccttaaatt caaactggca ttattgcagg
     9241 cgctgcctgc ggtaattgag aaatccgtgg aaccaatgaa gtctatcgac ggcatcaaaa
     9301 ttattcaggt cgatgggctg aatcgtggtg gcgctacggg tgatgcaaac acgggtaatg
     9361 tggggggcgg aaacctggca gaacaagcgt tatcagccgc actctcttac cgtacacagg
     9421 caccgctgat tgactccttg ctcaatgaaa ttggcgtttc aggcggctca ctggcggcat
     9481 tgacttcatc cttaagctca acaactcccg tcgaagaaaa agcagagtaa accctcttct
     9541 atgaataatc cctggcccaa aaagccgggg attttttata tctgcgttcc gctaaaaggt
     9601 gcaaatgatc aagctgttgc agcattttgc gtgactgctc ggggaaggca aaactgcctc
     9661 tgggaaagcg ttgcgcgggg tccggcgctc atcaacaatc ggggagtcag caaggggctg
     9721 aaacgggaaa gcccctcccg aggaaggggc cttgtataag gaaagggtta tgatgaagct
     9781 cgtcatcata ctgattgtgt tgttactcgt aagtttcgca gcttattaac agccaatcag
     9841 aggggggaga aatcctccct cacccttgtt cctttacttt aggttgaaaa aaaatcagcg
     9901 tcaataggcc cgcctgctac aaagcgagat ctgtgaaccg ctttccggtt agcctttttt
     9961 atcctgttgg atcttcttga tgatgttggt cgtcgagcaa ccgtcttcaa agttgagcac
    10021 cagcacttca ccaccgttgg cccagacttc tttactcccg gcaatctctt ctggtttata
    10081 gtcgccgcct ttcaccagta gatctggcaa gataccggcg atcaagcgct gcggcgtgtc
    10141 ctcttcaaac gacaccaccc agtcgaccgc ttccagtgcg cccagcacaa tcatacgctg
    10201 ttcgagctgg ttaacggggc gggaatcccc tttcagccgt ttggtggagg catcgctgtt
    10261 cacagcgaca atcaaacggt cacccagttt acgggcattt gccagataag agacgtgccc
    10321 agcgtgcagg atgtcaaaga caccgttggt catcaccact ttttcaccgc gtttacgcgc
    10381 tgccgctaca gcaagcttca gttcctcttc ggtcatcaca ccaaagccgg tatctgcacg
    10441 tccacgtaca gcgttttcca gctcgatcgg cgaaaccgtg gatgtcccca gtttgccgac
    10501 caccacacca gccgccgcat tggcaaagaa gcaggcttct tccagtgaat tacccgctgc
    10561 cagcgttgcc gccaggacgc caatcaccgt gtcgcccgca ccggtaacgt catacacttc
    10621 ctgcgcctgg gttggcatat gcagtggcgc tttacccggt tgcagcagcg acataccctg
    10681 ttcggaacgg gtcactaaca gagccgagag ttcgtaatcg gcaatcagtt tcatgccgcg
    10741 ctcaacaatc tcttcttcgg tcttacattt accgacaaca gcttcaaatt ccgagagatt
    10801 cggcgttaac agcgtagcgc cgcggtagcg ctcaaaatcg gtaccttttg gatcaatcag
    10861 caccggaaca cccgctttac gcgccagttg gatcatctgc tgtacgcttg ccagcgcacc
    10921 tttggcgtaa tcagaaagca ccagcgcgcc aatcgaactc agtgcctgat taatccgctc
    10981 gtgcagcggc tgcggatcaa ccccttcgaa accttcttcg aaatccagac ggatcagttg
    11041 ttggttgcgg gaaagtaccc gtaatttggt gatggtcgga tgcgtcggta cagaaacaaa
    11101 gtcgcatttg acgttgacgt cggccagaga tttactgagc gcgcgcgccg catcgtcaat
    11161 gcccgtcaac ccgaccaggc gtgcattagc accgagagaa gcgatattca tcgccacgtt
    11221 agccgcgccg cccggacgtt cttcgatggt attcacttta accacgggca ccggcgcttc
    11281 cggcgagata cggctggtgg gaccgtacca gtaacgatcc agcatcacat caccaaccac
    11341 catcactcct gcacgttcaa actctggcag cgttactttc attcctgtct cctgagagat
    11401 tcaaaatttg cgcgcgataa taccatactt cattcttcca ccagccactt ctgccagctt
    11461 gcccgcacca gttcacgctc tgcggtgaag caatcctccg acacatgacc cggcaattcc
    11521 tgtaatgcca gatgatgaag ttcatcgcgc agcgtcgtgt aagcacgggt cagcgccatc
    11581 gcttcctgct cttccataat gtcgttttgc gccagtagtt ccagaatgcg cacgttgtct
    11641 gaccagcgcg ttaacttcgg tttttcgtga gcgtagcgca acaccagata ttgagtaata
    11701 aattcgatat cggtaattcc gccttcatcg gctttgatat caaagcgatc gcgatgttta
    11761 ttgccgagat gggcacgcat tttctcgcgc atttcccgca cttctgtttg cagggttttg
    11821 ccttcgcgcg gcagcgtcat aatctcccgg cgtactgcgt caaagtgcgc ggtgagctgc
    11881 ggatcgccgt acactacacg cgcacgcacc agcgcctgat gctcccacgt ccaggcctcg
    11941 tttttctgat aatcagcaaa tgcttctgcg gatgtcacca gcattcccgc cgccccggac
    12001 ggacgcagtc gtgcatccac ttcatacaaa atgccggaag aggttcgcgt actgaacaga
    12061 tgcataatgc gttgcgccag acgcagataa aactgtcgcc cgtcgatttc ccgctcgccg
    12121 tcagtcatcg catccattgg gcaatcatgg aggaagatca ggtcaagatc ggaactgtag
    12181 cctaactccc agccacccag cttgccgtag ccgaccaccg caaaaccacg cccttcgcgt
    12241 tcgttcaggt gatttggctt accgtagcgg gcaaccattt gaacccacgc ctgctgaacg
    12301 acggcatcaa tcatggcttc tgccagccag gttaagtgat cgctcacttt cattaccggt
    12361 agcgtaccgg cgatatccgc tgccgcaatg cgtaacagct gcgcctgttt gaactgacgc
    12421 agcgcctcaa gctgttgttc ttcgtcatct tccggcacgc gcagcaaata ctggcgcagt
    12481 tcatcacggt aggcatccgt cgccgttggt tggtagaggg tatttggatc gagcaattca
    12541 tccagcaata atggataacg cgctagctgg ctggcaatca tcggcgatgc agcacacaga
    12601 gaaatcaaat gtttaagcgc cgcagggaat tcactaagca gctcaagata agtggtgcga
    12661 gtaacaatcc ccaccagcaa ggcggtaatg cgcgacagcg tgacagcagc gtcttcacgc
    12721 gcacagacat cacttagcag atgtggcatc agatggtcga gcacctgacg tcctcgcggc
    12781 ccgatggtgc gcttatccag ctctttgcgg aaatcagcaa tcagcattag cacctgtttg
    12841 cgatcatcct cgctaagatg cgccagcact ggcgtggtgt catcttcctg caacgcatcc
    12901 tgccacagct cacgccactg ttccgacagc gactcttcct gagtttcact ttcgtcgtcg
    12961 ccaatcaatt cattaaacac ccggcgcaca ttggccatat gtgcggtcag caccccggtc
    13021 aattgcggcc agtcagcaaa gtccatcgcc cacgccagcc gcgcacggtt aagctcatca
    13081 aaaggaagcg tctgggtttg ttcgtcgtta atgctttgca gcaggttttc cagacgccgc
    13141 aggaacagat acgccactcg caattgttca gcatcgtttt cagaaagcag atgcagcgcg
    13201 gcaatggcgc tgagcgttgg cagtaaagag cgcgattgca gcgacggttc gcgcccgccg
    13261 cgaatgagct ggaacacctg gacgataaat tcaatttcgc gaatgccgcc tgcgccaagt
    13321 ttgatgttgt cggtcaaacc gcgtcgacgc acttcacggg caatcattcc tttcatgttg
    13381 cgcagcgact gaatcacgct gaaatcgatg taacgacgga aaacaaacgg acgcagcatc
    13441 gcacgcaact cgttagcata gacgccgtcg ctatccccca taatccgcgc cttgaccatc
    13501 gcgtaacgct cccagtcgcg cccctgctcc tggtaataat cttccagcgc cgcaaaactc
    13561 agcaccagcg ggccactttc accaaacgga cgcagccgca tatccacgcg atagacgaaa
    13621 ccatcctgcg ttggttgatc cagcacttta atcagccgct gccccatgcg ggtaaaaaac
    13681 tgcgcgttat ccagttcccg gcgtccgccc tgggtacaac catgttccgg ccaggcaaaa
    13741 atcagatcga tatctgagga gaaattcagt tccccaccgc ccagcttacc catgcctaaa
    13801 atcagcagcg gttgcgcttc gccctgcgca ttgcacggcg ttccccattc gcggcagcag
    13861 gcgtcataca gccagtcacg cgccgcaaca atcagcgtct ccgccagatg gctgagctgc
    13921 tgcaatatgc tctcttcagt aaccagtgcc agcgtttgcg cccaggcgat gcgcaccata
    13981 atgcgccgcc ggaatagccg cagctcgcgc attaacccgg cttcgtcact cacattactg
    14041 agcgcctcct gcaaccatgc tgcgtaatgc tgccattcgt cggcctgcgg cggttggctt
    14101 tccagttccg tcagccactc tggatgcgca attatgctgt cctgcagaaa atcactgaaa
    14161 gtaagtactg acttcgcctg tgcgctaagt gattcctcgg ctaaaggctc tggcagccgc
    14221 tcaacaacgg tctgccagta ctgctgtaac ggtgaagaga gcggcttcat aagcgatttt
    14281 atccttgcct gattaacgtt ttccgctgtg caaccagaac ggttcctgat tgtttgcctc
    14341 attacggaaa tgttcaattt caatgcgttg cccggtcgca atagcgtgac gcagcccctg
    14401 ccagttctcc agccaggctt gcgcgacgac aggatcgtag taacccgcca gcaacaggat
    14461 tgagtcaata tcacgcgtca ggcgtggcaa ctggtcacgg tagcgatcgc ccaacggctg
    14521 gcagaaaacg cttttcagtt cagcggcatg gcgggaaaga tggatatcgg caaagcgttt
    14581 gaaggaatcg ctcattttac tctgcgcttt ggcatctaaa aacggctgcc aggctttgct
    14641 taccagccat tcggtcaatg ccagcttcgc cattgccgtt tcggtagagt agaccgccgt
    14701 cacggcagaa accgctgaag cgatggtcgc ttcgcattga gtcaataaat cacgtaagtg
    14761 agtgctcgct ttacgcggca caatgccacc gaacagcatc agcgtatgac ggaccaggcc
    14821 aatggctgcc agcacctgct ctttcgccgc atcattgccg cgtacccata gttcttcatg
    14881 atactgccat tgcgctaacg ccagctcgag cgccgcttcc agcccctgtt ccacatcagc
    14941 ttttgccgca acatgtaaaa tggtggtcgg tttgatttca cgcgccggat tgccctgcgc
    15001 cagatgatag ccacgcgccg ctttgctcag actgccctgg cgtaatccgg tttgcgatac
    15061 cagttggttc gccagtttca gcaccgcgcg cgtgtcgccg ctaagcagtt ccagttccag
    15121 ctcgcagata ggttcagcaa attcacccgc tttcacttcc ccctggtcga gggcgatttc
    15181 aatttggcta ccatcaacct ctaccagcca cttttcgcga taaaaatcgg tgctaaacag
    15241 cggctgcacg cgggaagcga gatcggtagg caattcgccg ttcggccaga cttccgtcgg
    15301 taactgcgcc aggtcgagcg tcggttcgct caacgccacg ttatattccg ggcgctggtg
    15361 taagccgcct gtcactcttc ctgcaacttt catggtcatc tcatagcgac cgttttcgcc
    15421 acgaatacgc aagcccatgt cgtgcccacg cagccagtta tccggcgttt cgtagtaaat
    15481 attcagcaac tgcacggggt catggtgctc gccgcccagc gtattgagat ggtcacgcaa
    15541 cgcctcaacg gcactgtgat taacaataaa ctttaattcg atttcctgag ccatagcctt
    15601 tacttatcgg ttatgtcaca taagtgacga tgaacggcga acttaatgcg atcttttttg
    15661 tcagtagata gtattttgcg ccaaattgcc atgcaacggg caatttgacg ggcgtaaaag
    15721 tttgaagcag tggcaagacc tgacacagag gattccgatt gatgtcgaat gctttgcgta
    15781 gcgacactga tgccactact atcgttccac tttccatgac aataacgaca gcctgatgcc
    15841 aaaattacgc ctgatcggat taactttact cgcacttagc gccactgccg tctcacacgc
    15901 tgaagaaacg cgctatgttt ctgatgaact gaatacctgg gtccgtagcg gtccgggaga
    15961 tcattatcgc ctcgtgggca cggttaacgc cggcgaggaa gtgaccttat tacaaactga
    16021 cgccaacacc aattatgccc aggtgaaaga cagctctggc cgtaccgcct ggatcccgtt
    16081 gaaacaactt agcacagagc caagcctgcg ctcccgtgtg ccagatctgg aaaatcaggt
    16141 caaaaccctg accgataaac tcaccaatat cgataacacc tggaaccaac gcaccgcaga
    16201 aatgcagcaa aaagtggcgc agagcgacag cgtgatcaac gggttaaaag aagaaaatca
    16261 gaaactgaaa aacgagctga ttgtcgcgca gaaaaaggtc gatgccgcca gcgtacagct
    16321 ggatgacaaa cagcgcacca tcatcatgca gtggtttatg tatggcggtg gcgtgctggg
    16381 gcttggcttg ctgcttggtc tggtactgcc gcacctgatc ccaagccgca aacgcaaaga
    16441 tcgctggatg aactaaatcg ccttcttcgt tgcactgtct tattattagg aattgtgtat
    16501 ttgacgaagg gatgatggcg tgaagattta tctggtcggt ggtgctgttc gggatgcatt
    16561 gttagggcta ccggtcaaag acagagattg ggtggtggtc ggcagtacgc cacaggagat
    16621 gctcgacgcg ggctaccagc aggtaggccg cgattttcct gtgtttctgc atccgcaaac
    16681 gcatgaagag tatgcactgg cacgtaccga acggaaatct ggttccggtt acaccggttt
    16741 tacctgctat gccgcaccgg atgtcacgct ggaagatgat cttaagcgtc gcgatctgac
    16801 cattaatgcg ctggcccagg acgataacgg cgagattatc gacccgtaca acggtctggg
    16861 cgatctgcaa aatcgtctgt tgcgccatgt ttcccccgct tttggcgaag atccgttacg
    16921 cgtgttgcgc gtagcgcgtt ttgctgcgcg ttatgcccac ctcagtttcc gtattgccga
    16981 tgaaactctg gcgctgatgc gcgagatgac ccatgcgggt gaactggaac acctgacgcc
    17041 tgaacgggta tggaaagaga cggaaaacgc cctcaccacc cgcaatcccc aggtgttctt
    17101 ccaggtactg cgcgattgcg gcgcactgcg cgttttattc ccggaaattg acgccctgtt
    17161 tggcgttccg gcccctgcca ggtggcatcc ggaaatcgac acgggtattc ataccttaat
    17221 gacgctctca atggcggcga tgctgagtcc gcaggtcgat gtccgtttcg caacgttatg
    17281 ccacgatctc ggtaaaggac tgacgccacc agaactttgg ccgcgtcatc atggtcatgg
    17341 cccggcgggt gtgaagttag tggaacaact atgccagcgt ctgcgcgtgc caaatgaaat
    17401 tcgcgattta gccagactgg tggctgagtt tcacgatctc atccacacct tcccaatgct
    17461 gaacccaaaa accatcgtca aactatttga ttccatcgac gcctggcgta aaccgcagcg
    17521 tgtcgagcaa ctggcgctga ccagcgaggc tgatgtgcgc ggcagaaccg gttttgaatc
    17581 agccgactac ccgcaaggcc gctggttgcg cgaagcctgg gaagtggcac agtcagtgcc
    17641 gacaaaagcc gtcgttgaag cgggatttaa aggtgtggag attcgcgagg agctgacccg
    17701 acggcggatt gcggcggtgg ctggctggaa ggaacagcgt tgcccaaaac ctgagtgagt
    17761 gttggatatt ttgccggatg cgacgtaagc gtcttatccg gctacatgtc aacgacagtt
    17821 gtaggcctga taagacgcgc gagcgtcgca tcaggcgttg attgccggat gcggcgtgaa
    17881 cgccttatcc ggcctacgca atctgataag acgcgtcagc atcatatccg acactgttct
    17941 tcacatcaaa agaacacaac gtacaccgca gccgccacaa taaagcgata aatggcgaac
    18001 gggataaacg aaatgcgctt aatcaattgc aggaaggttt taatcgctat cagcgccacc
    18061 acaaaagcgg tgataaaccc aacggcaaac atcgggatat cgccggtagt caggaagccc
    18121 cagcttttgt agagatcgag cgccgttgcg cccatcatca tcggcaccgc cagcaggaac
    18181 gagaactcgg aagcagcgta acggctcacc cccatcagca tcccacctga aatggtcgcc
    18241 ccggaacggg agaaacccgg ccacagcgcc agacactgga aacagccaat cataaacgcc
    18301 tgacgatagg tcatatcatc aagacccggc gcacgcggct ctttcggctt caggcattcg
    18361 gcggcaatca gcaacagacc gccaacgacc agtgcataca tcacatttat cgggttaaac
    18421 aatgatttaa tcgtgtcgtg gaacaacagc cccagtacca ccgccggaat catccccagc
    18481 aaaatgtgga tcagcgttaa acggcctttg ctttctcctt cgtgctgcaa cgggcgacca
    18541 aagtgaatac caataaggcc aaacagacgt cgccagaaca tgacaaccac tgccagaatt
    18601 gatcctaact ggatcacgac ttcaaaggtt ttcgccgtgt cgccctcaaa ccccaacaag
    18661 tgaccgacaa taatcatatg gcccgtgctg gataccggca gaaattctgt caatccttcg
    18721 accacaccca atattgccgc tatcagcagc gagtgcatat cgctcatcaa taaaccccta
    18781 aattataaaa atgtaacgct tgtccgaact actgcagtat gaccaggtta taaccgtttg
    18841 gtttaacagc tgtaaaatta attattttct ttcagattat tgccacgctc aatgattacg
    18901 ccaacattcg ccgcccgcgc cactgcgcct ggcttgctga gtttgatacg cacccacggc
    18961 gagttgaagc gtgctaacag caactccgcc acctcttcag ccacacgttc caccagcgca
    19021 aaacgcgccc cctcgacgtg gctgactacc gtttctgcaa tgtcagcgta actgaggcaa
    19081 tccgccacat catcactttt cgccgcttta cggttatccc acgccatttc gatatcgaac
    19141 actaacttct gttcgatggt ctgttcccag tcgtaaacac caatagtggt gattaccgaa
    19201 agttgctcta taaatacaat atccatcacg tcctgcctgc tttttggcta accggatacc
    19261 acttccggcg aaatgtgcgt attatccaca gattcatcgt tgaacacgaa ttttcaaaac
    19321 ggaacagctt atgagtgcaa tcgcgcctgg aatgatcctc atcgcgtacc tctgcggctc
    19381 catttccagt gccattctgg tttgccgctt gtgtgggctg cccgatccgc gaaccagcgg
    19441 ctccggcaat ccaggcgcaa ccaatgtgtt acgtatcggt ggcaagggag cagccgtagc
    19501 agtactgatt ttcgacgttc tgaaaggaat gttgcccgtc tggggcgcgt atgaattagg
    19561 tgtcagcccc ttctggctag gcttaattgc catcgccgcc tgtcttggac acatctggcc
    19621 cgttttcttc ggatttaaag gaggaaaagg cgttgctacc gcttttggtg ccatcgcacc
    19681 cattggctgg gatctcaccg gagtaatggc gggaacctgg ttactgaccg tgctattgag
    19741 cggatattcg tcgctggggg cgattgtcag tgcactgatt gctccgtttt atgtctggtg
    19801 gtttaagcca caattcacct tcccggtttc gatgctctct tgcctgatcc tgctgcgcca
    19861 tcatgacaac atccagcgtc tgtggcgtcg tcaggagaca aaaatctgga cgaaattcaa
    19921 aagaaagcgc gaaaaggatc ccgagtgatt tctggtggag ctacataacc tgatagcctt
    19981 cgtcgggctt gcccagccgt tgctggcacc atgccgccag aaattccacg cagacacgta
    20041 atttcatgct gcgatagagc ggctcccggt aaacagccca gatattggcg ctttgtgcat
    20101 actccggcaa tactcgcacc agtttgccac tctccagaaa cggcaacaca tcccactcgg
    20161 aacgcaacat aatccctttg ccctccagcg cccattgcaa cactatctcg ccgctattgg
    20221 aggaaagatg cccgcttacc ttcaccgatt ttttctcctg accgttcccc aactcccata
    20281 ttccgtgggt catatcgcgc tctttggtca ccaggcagtc atgacgactt aattcctgta
    20341 aggattgtgg ctgcggatat ttttgcagat actcaggtgc tgcgcataat attcttttat
    20401 tttttgtcaa aagatgcgca atataataat caggaattgc gtcattaata cgaatatcca
    20461 gatcaatatt atcctgcact aaatcaattt gccgatcgaa caattcaaaa tgcacctgta
    20521 actcaggata attgcgcatc agttcggtaa tggctggagc aatatggctg cgcccaaaac
    20581 caaaactgca accaatgcgg atcatcccct ccgggcgcgt tttgatttgc gtgacgtcat
    20641 cgaccagtcg ctgatattgg gtgaggattt ccagcgcatg ttcgtaacaa cgttggccac
    20701 tttcagtcag cgccacgccg cgagcggagc ggttcagcag agttgttgcc agcgtatttt
    20761 caaggatttg gatccgcttg gtgacaaatg caggcgtttg cccaagcgtc gccgctgcag
    20821 cactaaaact cccgctatgg acgatttcca ccagtacctg taagtctttg gctaaaggcc
    20881 agctatttag cattgttaaa tatccgcagt tttgataaac gcagtgtatt tcgcaaaaca
    20941 ttaccattcc ctgctctggc gcaatattcg ttcttatttt cgcgttgatt cacgaactgt
    21001 taattacata ttcacggtgg caaaaaatat aaaaccacat ttttagtggt agtttgtggc
    21061 ggtgaatttt tccagacaaa tacataaact ggagttgcca tgatgagcga aagtaataag
    21121 caacaggcag tgaataagtt gacggagatt gtcgctaact ttaccgccat gatttctacc
    21181 cgaatgcctg atgacgtggt ggataaacta aaacagctaa aggatgccga aacatcgtcg
    21241 atgggaaaaa tcatctacca cacgatgttc gataacatgc aaaaagcgat cgacctgaat
    21301 cgtcctgcct gtcaggacac cggcgaaatc atgttttttg ttaaggtcgg ttcccgtttc
    21361 ccactgcttg gcgagctgca aagcatcctc aaacaagccg tggaagaggc aaccgtcaaa
    21421 gcgccactgc gtcacaatgc ggtagaaatt tttgacgaag tgaacaccgg caaaaatacc
    21481 ggcagcggcg taccgtgggt cacctgggat atcgtccccg acggtgacga tgcggaaatc
    21541 gaagtttaca tggcaggcgg cggctgcaca ctacctggtc gttcgaaagt gttaatgccg
    21601 tcggaaggat acgaaggcgt ggtgaaattc gtcttcgaaa atatctccac cctcgccgta
    21661 aacgcctgtc caccggtact ggtaggcgtg ggcatcgcca cctcggtgga aaccgccgct
    21721 gtgctctcac gtaaagccat tttacgccca attggcagcc gccaccccaa tccgaaagcg
    21781 gcagagctgg agctacgcct ggaagaagga ctcaaccgtc tggggattgg tccacaaggg
    21841 ctgactggca acagttcagt gatgggcgta catatcgaat ctgccgcccg ccatccgtca
    21901 accatcggcg ttgctgtttc tacaggctgc tgggcgcatc gtcgcggcac actgctggtt
    21961 catgccgatc tcacctttga aaatctgtct cacacccgga gcgcgttatg aaaaagatcc
    22021 tgacaacccc gatcaaagct gaagatctgc aagatattcg cgtcggcgat gtgatctacc
    22081 tgaccggtac gctggtgacc tgccgtgacg tttgtcaccg tcgcttgatc gaactcaaac
    22141 gtccgatccc ttacgatctc aacggcaaag cgattttcca cgctggcccc atcgtgcgca
    22201 aaaacggcga caaatgggag atggtatccg tcggcccgac aaccagtatg cgtatggaaa
    22261 gttttgaacg tgaatttatc gaacagaccg gcgtgaaact ggtggtcggc aaaggcggaa
    22321 tggggccgct gaccgaagaa ggctgccaga aattcaaggc gctgcatgtg attttcccgg
    22381 caggctgcgc agtactggcg gcaacccagg tggaagagat tgaagaagtg cactggacag
    22441 aactcgggat gccggagtcg ctgtgggtct gccgggtcaa agagttcggt ccgctgattg
    22501 tttctattga tacccacggc aacaacctga tagccgaaaa caaaaagctg ttcgccgaac
    22561 gccgcgatcc catcgtggaa gagatttgcg agcacgtcca ctacatcaaa taaccctccc
    22621 ggagaggctc accccctctc ctttttcgca ggcataatac gatgaaacct tccactgaat
    22681 ggtggcgata ccttgcgccg ctggcggtca tcgccattat tgctctaatt ccggttcccg
    22741 cagggctgga gagtcatacc tggctctact ttgccgtttt tactggcgtg atcgttggac
    22801 tgatcctcga acccgtgccg ggtgccgtgg tggcgatggt gggtatctcc attatcgcca
    22861 ttctctcgcc gtggctgctg tttagcccgg agcagctcgc ccagccaggt tttaagttca
    22921 ccgccaaatc cctctcctgg gcggtttctg gtttttctaa ttcggttatc tggctgattt
    22981 tcgccgcctt tatgtttggc acaggctatg aaaaaaccgg gcttggacgc cgcattgcgc
    23041 tgattctggt gaaaaagatg ggacatcgca cgctgtttct cggctatgcg gtgatgttct
    23101 ccgagctgat cctggcacct gtaacaccgt ccaactcggc gcgcggtgcg gggattatct
    23161 atcccatcat ccgtaacctg ccaccgctct atcaatcaca accaaacgac agcagttcgc
    23221 gcagcattgg ctcgtacatc atgtggatgg ggattgttgc cgactgcgtg accagcgcca
    23281 ttttcctgac ggcgatggca cctaacttgc tgttaattgg cctgatgaaa agcgcatctc
    23341 acgccacact gagttggggc gactggttcc tcgggatgtt gccgctcagt attttactgg
    23401 ttctgctggt tccctggctg gcttacgtgc tgtacccgcc ggtactgaag tctggtgatc
    23461 aggtgccgcg ctgggcagag acggaactgc aggcaatggg cccgctctgt tcgcgtgaaa
    23521 aacggatgct ggggctgatg gtaggcgcgc tggtgctgtg gattttcggc ggtgattata
    23581 tcgatgccgc gatggtcggt tacagcgtgg tggcgctgat gctgcttctg cgcattatca
    23641 gttgggacga cattgtcagt aataaagccg cgtggaacgt tttcttctgg ctggcctcgc
    23701 ttatcaccct cgctaccgga ctcaacaaca ccggttttat tagctggttt ggcaaactgt
    23761 tagcaggcag cttaagcggt tattcgccaa cgatagtgat ggtggcgttg attgtggtgt
    23821 tttatctact gcgctacttt ttcgccagcg ccacggcgta tacctccgct ctcgcgccga
    23881 tgatgattgc cgccgcgctg gcgatgccgg aaatcccgct gccggtgttc tgcctgatgg
    23941 ttggcgcggc aattggtctg gggagcattc ttacgccata cgccaccgga cccagtccga
    24001 tttactacgg tagtggttat ctgccaacgg tggattactg gcgactgggg gcaatttttg
    24061 ggctgatatt cctcgtgttg ctggtgatta ccggcttact gtggatgccc gtggtgttgc
    24121 tttaagtgag gacggtgccg gaagtgactc tccggcacct aagagtttta cgcggccggt
    24181 aactctgcca gcggccagcg cggacgcacg ctaacgccaa gatccgccgt cgcgcctgct
    24241 ttaaaccgca ccattccggc ataggcgatc atcgcgccgt tatcagtaca aaattccgga
    24301 cgcgcgtaga acacttcgcc acggcgtttt ttcatcattt ccgccagctt cgcccgtaac
    24361 gtgcggttag cactcacgcc acctgccatt accagtcgct taaagcccgt ctgatccagc
    24421 gctcgcttac acttaatcat caacgtatcg accaccgcat cttcaaaggc gcgggcgata
    24481 tcagcacgcg tctggtcgtc ggtgccgtta tcacgaatgg tatttgccgc gaaggttttc
    24541 agaccagaaa agctgaaatc cagccctgga cggtcggtca tcgggcgcgg gaagacaaag
    24601 cgcccggctg taccctgagc cgccattttc gacagtaatg gcccgccagg ataatccagc
    24661 cccagcagct tcgcggtttt atcaaacgct tcaccagccg catcatcgat agactcgccg
    24721 agcagctcgt actgaccaat gccagtcacg ctgattaact gcgtatgacc gccggaaacc
    24781 agcagcgcca caaacggaaa ttccggtggg ttatcttcca gcatcggcgc taacagatgc
    24841 ccttccatat ggtgtacagg gatcgccgga acgttccagg caaacgccag agaacgcccc
    24901 acggtcgcgc caaccagcag tgcgccgact aatccagggc ctgcggtata ggccacagca
    24961 tcaatgtctt ttgccgttaa accagactcc tttagcgccg cctggatcaa cggtacggtt
    25021 ttacgcacat gatcgcggga ggccagctca ggcactacgc cgccgtagtc agcgtgcaat
    25081 ttcacctgac tatacaattg gttggctaac aaaccttttt catcgtcgta aatggcgatg
    25141 ccggtttcat cgcaggaagt ttcaataccc agtacacgca tgacttgttt tacctcgctt
    25201 tattaccgcg cagtgtagga ccaatgcggg ttgatgtaaa actttgttcg cccctggaga
    25261 aagcctcgtg tatactcctc acccttataa aagtcccttt caaaaaaggc cgcggtgctt
    25321 tacaaagcag cagcaattgc agtaaaattc cgcaccattt tgaaataagc tggcgttgat
    25381 gccagcggca aaccgaatta atcaaaggtg agaggcacat gccggtaatt aaagtacgtg
    25441 aaaacgagcc gttcgacgta gctctgcgtc gcttcaagcg ttcctgcgaa aaagcaggtg
    25501 ttctggcgga agttcgtcgt cgtgagttct atgaaaaacc gactaccgaa cgtaagcgcg
    25561 ctaaagcttc tgcagtgaaa cgtcacgcga agaaactggc tcgcgaaaac gcacgccgca
    25621 ctcgtctgta ctaattcccc gagagcgttg ctctccgatc agaccgagtt gtagttgtaa
    25681 ggccgtgctt ccgaaaggaa tgcgcggctt attttcgttt atgaattgct aaaaatcggg
    25741 gcctatggct ggacgaatcc cacgcgtatt cattaatgat ctgctggcac gcactgacat
    25801 cgtcgatctg atcgatgccc gtgtgaagct gaaaaagcag ggcaagaatt tccacgcgtg
    25861 ttgtccattc cacaacgaga aaaccccgtc attcaccgtt aacggtgaga aacagtttta
    25921 ccactgcttt ggatgtggcg cgcacggcaa cgcgatcgac ttcctgatga actacgacaa
    25981 gcttgagttc gtcgaaacgg tcgaagagct ggcggcaatg cacaatcttg aagtgccatt
    26041 tgaagcaggt agcggcccca gccagatcga gcgccatcaa cgacaaacgc tttatcagtt
    26101 gatggacggt ctgaatacgt tttaccaaca atctttacag caacctgttg ccacgtctgc
    26161 gcgccagtat ctggaaaaac gcggattaag ccacgaggtt atcgcccgct ttgcgattgg
    26221 ttttgccccc cctggctggg acaacgtcct gaagcggttt ggcggcaatc cagaaaatcg
    26281 ccagtcattg attgatgcgg gcatgttggt cactaacgat caggggcgca gttacgaccg
    26341 tttccgcgag cgggtgatgt tccccattcg cgataaacgc ggtcgggtga ttggttttgg
    26401 cgggcgtgtg ctgggcaacg atacacccaa atacctgaac tcgccggaaa cggacatttt
    26461 ccataaaggc cgccagcttt acggtcttta tgaagcgcag caggataacg ctgaacccaa
    26521 tcgtctgctt gtggtcgaag gctatatgga cgtggtggcg ctggcgcaat acggcattaa
    26581 ttacgccgtt gcgtcgttag gtacgtcaac caccgccgat cacatacaac tgttgttccg
    26641 cgcgaccaac aatgtcattt gctgttatga cggcgaccgt gcaggccgcg atgccgcctg
    26701 gcgagcgctg gaaacggcgc tgccttacat gacagacggt cgtcagctac gctttatgtt
    26761 tttgcctgat ggcgaagacc ctgacacgct ggtacgaaaa gaaggtaaac aagcgtttga
    26821 agcgcggatg gagcaggcga tgccactctc cgcatttctg tttaacagtc tgatgccgca
    26881 agttgatctg agtacccctg acgggcgcgc acgtttgagt acgctggcac tgccactgat
    26941 atcgcaagtg ccgggcgaaa cgctgcgaat atatcttcgt caggaattag gcaacaaatt
    27001 aggcatactt gatgacaacc agcttgaacg attaatgcca aaagcggcag agagcggcgt
    27061 ttctcgccct gttccacagc taaaacgcac gaccatgcgt atacttatag ggttgctggt
    27121 gcaaaatcca gaattagcga cgttggtccc gccgcttgag aatctggatg aaaataagct
    27181 ccctggactt ggcttattca gagaactggt caacacttgt ctctctcagc caggtctgac
    27241 caccgggcaa cttttagagc actatcgtgg tacaaataat gctgccaccc ttgaaaaact
    27301 gtcgatgtgg gacgatatag cagataagaa tattgctgag caaaccttca ccgactcact
    27361 caaccatatg tttgattcgc tgcttgaact gcgccaggaa gagttaatcg ctcgtgagcg
    27421 cacgcatggt ttaagcaacg aagaacgcct ggagctctgg acattaaacc aggagctggc
    27481 gaaaaagtga tttaacggct taagtgccga agagcgatcg ggaagccccc gacagccgca
    27541 ctgagaggca gcggcaaata tataagtacg ccctcgtaat tatcgttggc ggtaaacaac
    27601 cgttggattt cagcgttaac ggctgaagga catcgggtca atcgcccaac accaacctca
    27661 tgaaataagt gtggataccg tcttatggag caaaacccgc agtcacagct gaaacttctt
    27721 gtcacccgtg gtaaggagca aggctatctg acctatgccg aggtcaatga ccatctgccg
    27781 gaagatatcg tcgattcaga tcagatcgaa gacatcatcc aaatgatcaa cgacatgggc
    27841 attcaggtga tggaagaagc accggatgcc gatgatctga tgctggctga aaacaccgcg
    27901 gacgaagatg ctgccgaagc cgccgcgcag gtgctttcca gcgtggaatc tgaaatcggg
    27961 cgcacgactg acccggtacg catgtacatg cgtgaaatgg gcaccgttga actgttgacc
    28021 cgcgaaggcg aaattgacat cgctaagcgt attgaagacg ggatcaacca ggttcaatgc
    28081 tccgttgctg aatatccgga agcgatcacc tatctgctgg aacagtacga tcgtgttgaa
    28141 gcagaagaag cgcgtctgtc cgatctgatc accggctttg ttgacccgaa cgcagaagaa
    28201 gatctggcac ctaccgccac tcacgtcggt tctgagcttt cccaggaaga tctggacgat
    28261 gacgaagatg aagacgaaga agatggcgat gacgacagcg ccgatgatga caacagcatc
    28321 gacccggaac tggctcgcga aaaatttgcg gaactgcgcg ctcagtacgt tgtaacgcgt
    28381 gacaccatca aagcgaaagg tcgcagtcac gctgccgctc aggaagagat cctgaaactg
    28441 tctgaagtat tcaaacagtt ccgcctggtg ccgaagcagt ttgactacct ggtcaacagc
    28501 atgcgcgtca tgatggaccg cgttcgtacg caagaacgtc tgatcatgaa gctctgcgtt
    28561 gagcagtgca aaatgccgaa gaaaaacttc attaccctgt ttaccggcaa cgaaaccagc
    28621 gatacctggt tcaacgcggc aattgcaatg aacaagccgt ggtcggaaaa actgcacgat
    28681 gtctctgaag aagtgcatcg cgccctgcag aaactgcagc agattgaaga agaaaccggc
    28741 ctgaccatcg agcaggttaa agatatcaac cgtcgtatgt ccatcggtga agcgaaagcc
    28801 cgccgtgcga agaaagagat ggttgaagcg aacttacgtc tggttatttc tatcgccaag
    28861 aaatacacca accgtggctt gcagttcctt gacctgattc aggaaggcaa catcggtctg
    28921 atgaaagcgg ttgataaatt cgaataccgc cgtggttaca agttctccac ctacgcaacc
    28981 tggtggatcc gtcaggcgat cacccgctct atcgcggatc aggcgcgcac catccgtatt
    29041 ccggtgcata tgattgagac tatcaacaaa ctcaaccgta tttctcgcca gatgctgcaa
    29101 gagatgggcc gtgaaccaac gccggaagaa ctggctgaac gtatgctgat gccggaagac
    29161 aagatccgca aagtgctgaa gatcgccaaa gagccaatct ccatggaaac gccgatcggt
    29221 gatgatgaag attcgcatct gggggatttc atcgaggata ccaccctcga gctgccgctg
    29281 gattctgcga ccaccgaaag cctgcgtgcg gcaacgcacg acgtgctggc tggcctgacc
    29341 gcgcgtgaag cgaaagttct gcgtatgcgt ttcggtatcg atatgaacac cgaccacacg
    29401 ctggaagaag tgggtaaaca gttcgacgtt acccgcgaac gtatccgtca gatcgaagcg
    29461 aaggcgctgc gcaaactgcg tcacccgagc cgttctgaag tgctgcgtag cttcctggac
    29521 gattaatcgg taggccggat caggtgttac gccgcacccg gcactagtcc ctccgcacaa
    29581 acgccacctt ttcggtggcg tttttatcgc ccacgcacca ccagtgcctg gtccagctcg
    29641 cgatacgctt caaccagttt ctccagtgaa acgcgactta aaccgctggg atttggcagc
    29701 acccaaatct gcgtcgaacc aatggtgaga gtttgtttcc cccactgtgc accgcgctgg
    29761 ctgaatccct gttcatatgc ttgtttgccc agaatcgcca acgcctgcgg ctgataatct
    29821 tcaatttttt caatcagctt acgcccgcct gcgtgtagct cctgctttga aacttcattg
    29881 gcttgcaccg ttggacggtc taccagtttg gtgacgccac aacgataatc cagcagatgc
    29941 tgtgcctcct gcggcttcaa ctgacggtcg gtaaacccgg cctgatatat caccttccag
    30001 aagcgatttg ccggatgagc aaagggaaaa ccagtcccgg cggatgaaag cccagggttg
    30061 ataccgcaaa acacgacccg taaccctgga gccaaaatat cctcaaccat atttactcct
    30121 ttctaacatc accaggaaaa gtataacgga ttgaaaatac attgtttata aaaacagcag
    30181 gcgcgcggta atggctggat tgcgacacgg agttacttta taatccgcta ccatggcccc
    30241 ttagctcagt ggttagagca ggcgactcat aatcgcttgg tcgctggttc aagtccagca
    30301 ggggccacca gatatagcaa aggctgacga gaaatcgtca gcctttttct ttttatatat
    30361 cagttacttt gcgtgccagt aagccgctgc acgtacccgc tgtgggtcat actgttccgc
    30421 ttcaaagcgg cggcttaaat tcttaacgac tttgccttcg ccggttatcc agatgaagta
    30481 atcatcggca gggatttgca tctgcgccag acgcgcatct accgcctgct catcatgcgc
    30541 cagccattcg atattaaaac catcaaggtg cgcgagataa tcctgacagg cgttatcccg
    30601 cacgctaacc agcgcactaa cttgcggttt aacggcaagt ttgctcaacg tttccaggcg
    30661 gcggcgcagt gcgggcattc cggattcatc gcagacatac agctgatacg cgtaatcctc
    30721 tggcaccacc agcgaaccgc gcggacctgc caccgtgagt ttatcgcccg gttgcgcctg
    30781 cattgcccag ctgctggcga cgccaccttc gtgaataaag aaatcaatcg ccagttcatg
    30841 gcgtagttcg tcatacagcg gcgtatagtc acgcgacggt gggcgtggtc cttccggcca
    30901 gacgatgccc tcttccgtta ccgttggcgg cacaaagtga gcatcagatt gaggaaagaa
    30961 gagtttgctg tgatcgtcaa agccgcgcga tgtaaaaccg tccagcgcct cgccgccaag
    31021 gacaatgcgc tgaaaaccga cgctaatacg ctcaacgcgt agcacagtca attcacggaa
    31081 gcgcagatca ttgcgaacgc gctgtgggta gcggggggtg ttattcattg ttatcgcctt
    31141 c
//
LOCUS       DAZKGF010000035        30965 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZKGF010000035 DAZKGF010000000
VERSION     DAZKGF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.035"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(154..717)
                     /gene="fimA"
                     /locus_tag="ACHOQV_001595"
     CDS             complement(154..717)
                     /gene="fimA"
                     /locus_tag="ACHOQV_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9629989.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            complement(1151..1417)
                     /locus_tag="ACHOQV_001596"
                     /pseudo
     CDS             complement(1151..1417)
                     /locus_tag="ACHOQV_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1693..3009)
                     /gene="hipA"
                     /locus_tag="ACHOQV_001597"
     CDS             complement(1693..3009)
                     /gene="hipA"
                     /locus_tag="ACHOQV_001597"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFP9629990.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPQVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREWY"
     gene            complement(3009..3275)
                     /gene="hipB"
                     /locus_tag="ACHOQV_001598"
     CDS             complement(3009..3275)
                     /gene="hipB"
                     /locus_tag="ACHOQV_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFP9629991.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            complement(3468..5357)
                     /locus_tag="ACHOQV_001599"
                     /pseudo
     CDS             complement(3468..5357)
                     /locus_tag="ACHOQV_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            complement(5507..7099)
                     /gene="lsrK"
                     /locus_tag="ACHOQV_001600"
     CDS             complement(5507..7099)
                     /gene="lsrK"
                     /locus_tag="ACHOQV_001600"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFP9629992.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDPEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVNELKELHNNTFENKVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLIDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(7178..8131)
                     /gene="lsrR"
                     /locus_tag="ACHOQV_001601"
     CDS             complement(7178..8131)
                     /gene="lsrR"
                     /locus_tag="ACHOQV_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFP9629993.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            8380..9915
                     /gene="lsrA"
                     /locus_tag="ACHOQV_001602"
     CDS             8380..9915
                     /gene="lsrA"
                     /locus_tag="ACHOQV_001602"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFP9629994.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYTRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSIQKMNNLLVALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFRRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPALREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINRLSTGD
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            9909..10937
                     /gene="lsrC"
                     /locus_tag="ACHOQV_001603"
     CDS             9909..10937
                     /gene="lsrC"
                     /locus_tag="ACHOQV_001603"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFP9629995.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYVTGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTVLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGMVLLAVLVFDGRLRCALERNLRRQKYARFMMPPPSVKPAS
                     SGKKREAA"
     gene            10937..11929
                     /gene="lsrD"
                     /locus_tag="ACHOQV_001604"
     CDS             10937..11929
                     /gene="lsrD"
                     /locus_tag="ACHOQV_001604"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFP9629996.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVAVYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            11941..12963
                     /gene="lsrB"
                     /locus_tag="ACHOQV_001605"
     CDS             11941..12963
                     /gene="lsrB"
                     /locus_tag="ACHOQV_001605"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFP9629997.1"
                     /translation="MTLHRFKKIALLSALGIAVISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            12990..13865
                     /gene="lsrF"
                     /locus_tag="ACHOQV_001606"
     CDS             12990..13865
                     /gene="lsrF"
                     /locus_tag="ACHOQV_001606"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFP9629998.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKAGIPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVDKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            13889..14179
                     /gene="lsrG"
                     /locus_tag="ACHOQV_001607"
     CDS             13889..14179
                     /gene="lsrG"
                     /locus_tag="ACHOQV_001607"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFP9629999.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            14236..14994
                     /gene="tam"
                     /locus_tag="ACHOQV_001608"
     CDS             14236..14994
                     /gene="tam"
                     /locus_tag="ACHOQV_001608"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFP9630000.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STTLLHQRWPAARITGIDSSPAMIAEARSTLPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQSIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(14998..15912)
                     /gene="yneE"
                     /locus_tag="ACHOQV_001609"
     CDS             complement(14998..15912)
                     /gene="yneE"
                     /locus_tag="ACHOQV_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFP9630001.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISVVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(16109..17560)
                     /gene="uxaB"
                     /locus_tag="ACHOQV_001610"
     CDS             complement(16109..17560)
                     /gene="uxaB"
                     /locus_tag="ACHOQV_001610"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP9630002.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTGLVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILKKGMREAVRPLC"
     gene            complement(17788..19206)
                     /locus_tag="ACHOQV_001611"
     CDS             complement(17788..19206)
                     /locus_tag="ACHOQV_001611"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9630003.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(19345..19704)
                     /gene="yneG"
                     /locus_tag="ACHOQV_001612"
     CDS             complement(19345..19704)
                     /gene="yneG"
                     /locus_tag="ACHOQV_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFP9630004.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(19704..20630)
                     /gene="glsB"
                     /locus_tag="ACHOQV_001613"
     CDS             complement(19704..20630)
                     /gene="glsB"
                     /locus_tag="ACHOQV_001613"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFP9630005.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRMGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCIELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(20694..22082)
                     /gene="sad"
                     /locus_tag="ACHOQV_001614"
     CDS             complement(20694..22082)
                     /gene="sad"
                     /locus_tag="ACHOQV_001614"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFP9630006.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRVRSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNNGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETRARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            22183..23058
                     /gene="yneJ"
                     /locus_tag="ACHOQV_001615"
     CDS             22183..23058
                     /gene="yneJ"
                     /locus_tag="ACHOQV_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9630007.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAATKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPAMLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQS"
     gene            23142..23261
                     /gene="yneK"
                     /locus_tag="ACHOQV_001616"
     CDS             23142..23261
                     /gene="yneK"
                     /locus_tag="ACHOQV_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFP9630008.1"
                     /translation="MVTPVSISNFISLPDDFPVRNIAPQVKEEQAPFLIVIKM"
     gene            23403..24593
                     /gene="ydeA"
                     /locus_tag="ACHOQV_001617"
     CDS             23403..24593
                     /gene="ydeA"
                     /locus_tag="ACHOQV_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFP9630009.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGELITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(24618..25283)
                     /gene="marC"
                     /locus_tag="ACHOQV_001618"
     CDS             complement(24618..25283)
                     /gene="marC"
                     /locus_tag="ACHOQV_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFP9630010.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            25495..25929
                     /gene="marR"
                     /locus_tag="ACHOQV_001619"
     CDS             25495..25929
                     /gene="marR"
                     /locus_tag="ACHOQV_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFP9630011.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            25950..26333
                     /gene="marA"
                     /locus_tag="ACHOQV_001620"
     CDS             25950..26333
                     /gene="marA"
                     /locus_tag="ACHOQV_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFP9630012.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            26365..26583
                     /gene="marB"
                     /locus_tag="ACHOQV_001621"
     CDS             26365..26583
                     /gene="marB"
                     /locus_tag="ACHOQV_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFP9630013.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPTSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(26614..27513)
                     /gene="eamA"
                     /locus_tag="ACHOQV_001622"
     CDS             complement(26614..27513)
                     /gene="eamA"
                     /locus_tag="ACHOQV_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFP9630014.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            27708..28895
                     /gene="ydeE"
                     /locus_tag="ACHOQV_001623"
     CDS             27708..28895
                     /gene="ydeE"
                     /locus_tag="ACHOQV_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFP9630015.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYNLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRNEKIIATETGSAWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            29022..29117
                     /gene="mgtS"
                     /locus_tag="ACHOQV_001624"
     CDS             29022..29117
                     /gene="mgtS"
                     /locus_tag="ACHOQV_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFP9630016.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            29117..29221
                     /gene="mgtT"
                     /locus_tag="ACHOQV_001625"
     CDS             29117..29221
                     /gene="mgtT"
                     /locus_tag="ACHOQV_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFP9630017.1"
                     /translation="MNGDNPSPNRPLVTTVYKGPDFYDGEKKPPVNRR"
     gene            complement(29336..30226)
                     /gene="dgcZ"
                     /locus_tag="ACHOQV_001626"
     CDS             complement(29336..30226)
                     /gene="dgcZ"
                     /locus_tag="ACHOQV_001626"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFP9630018.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYELCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRVGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(30481..30873)
                     /gene="ydeI"
                     /locus_tag="ACHOQV_001627"
     CDS             complement(30481..30873)
                     /gene="ydeI"
                     /locus_tag="ACHOQV_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFP9630019.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         7824 a         7838 c         7352 g         7951 t
ORIGIN      
        1 tagatattat gtgatttttt cataccgctc caaaacacaa actattcctc attaataatg
       61 agatgaacat tggtgaattt ctcgggtgtg cgtagccatg ccacacacac cctgtttcaa
      121 tttcagatga cgtatagcca tcttaagatt gaattataga tattgaactt tgaaggtcgc
      181 atcagcgttt gcagtaccag ctactgactg cccgagagca atataacgag cctggaacgg
      241 caatatattc gtgccatcaa tcaagtcagt tttagcgcta aaagtggcac catcaagtat
      301 taatacctcc cccgtacggt ccaaaatttg aataccgaca ttttgggcag agcctgccgc
      361 tgaactctgc agcgctaacg tatcgtcatt actggtgaca gtagtaccca ggaatgcaac
      421 agctgcatta ctggaaacat tggtatcaca atcattcagc ttgatattga agccaacggc
      481 ggagctgagg tcacccgctt tagccagacg agaagaacgg acctgaccca gctcaaccgt
      541 ttggttcatt gattctgaat caatcgcaca agcggcattt accacttcac ctttaaaatg
      601 aataacaccg ccattaacag tggtcgttac tgattcatca ccctcggctg cgcttaccgc
      661 agcagacgac atcgccaata cagaaacgac aatcataccg acatgtttga gtttcataat
      721 tctccaaaat ttatatttaa gtagctgaat aaagagagtt aacaattaag tcattctgtt
      781 attgcttata aaacaagaat cattcttaat tggtagttgc cctgagaata tagtttccag
      841 ggttattaca tttcacgaag aacgataata tctttaaatt acaaggacac ctaatcacaa
      901 caagtttatt gcagcacttg ttgtagagag tatttgcaaa gacattttat gtcgctaaat
      961 aacatgacga tttcattatg catgttattt ataaaccgtg actaaacaga atattattgc
     1021 gaacaaggga agtgtcagga aaggtcaaga gcgaccaatg gtttatatgg ctataaatca
     1081 ttagccatag ttaatgtttg cctggtgtaa ctaacacgga attgctctaa acagataagt
     1141 gtagggttta ttaaagctga ccgacagact ttcttaacct ggtaatatct ctcttaggtg
     1201 actctccaaa catccgcgaa tattcccgac tgaaatggga taagctttcg taaccgacag
     1261 cataggctgc agtggtgaca tcgtaatgtt cattcagcat cagtcgcctg gcttcattga
     1321 ggcttagcca tttccgatac cggagtggac tcattcccgc aagctgtctg aaatgttgat
     1381 ggaaagaggg ggtgttcatt tgcacgcggt ttgccagcct gactcttcaa cgctgagaag
     1441 atgcggcaaa cggcaaacct gcataccagc cagcctatat ccggtatgca caggccaatt
     1501 atgaaagagg agaggcttta tgttctggaa ccttgaagta ataacgtcga ccggaaaatc
     1561 actctgcatc atcactcaga ttcaatttaa cataatatac attatgcgca ccaacataaa
     1621 ccaagggaga atccagccgt tggcggtcat gattgtcctg ctcattaaca acggccacac
     1681 cccatatctt acttaatacc attctcggct taaccgacgg tgcaacctca acacattggt
     1741 ttcaactgcc gtcaccacgt tctccggaaa atctgtcggt aatgaagtct tcacgttatc
     1801 cagtgctgcc ggaatcattc tggcaaagtc actcaggatt tcatgcatct gcacttgcgg
     1861 aaatttcagc acctttgctg tcgccagaaa atgtcgcgga taaattttat cgattgccgt
     1921 ttttttgcct ttggatgcgt taagccccat tgccagtttg agatcgctga tgtgtattcc
     1981 tgcaccaccc aataccggaa atgctgaaat gatgtcgtaa aatggcgtga gtcgataact
     2041 gccgccagcc tgaataaata cggagaagtt ttttgcatgg ccatccgttg cgccaatcaa
     2101 ccactggaac acctgaaact tcataaaatc atagcgatct ttcagcgcct cgctggaccc
     2161 catcaaaaaa gccatgatcc gcgcgatgcc tgggcctcca tctgattcat atttcaccga
     2221 tgaaggtaaa ccgaatgtct gacacatatc ctcctgtggc aagcgaagta aaaccgttcg
     2281 ctcagcattc caacgcctgt caaaacgttc gaccgctaac gcgcgcacat ttcccgcttt
     2341 aatgatttct gcgtccggca cattcaaccc aagttcttta gccagcagca gacagtaata
     2401 ctcattatca acgctttggc tgagatcgag cgtcgcattg ggctgcctga tttcgccaat
     2461 cggtaattta atgatgtgcg tcgtcggcgt tattcctttc ggaatgcacc agtcattgcc
     2521 tattctgagc agcgctgtct tctcctgcgc gccagcaacc gagatgcgaa agtcattttc
     2581 ttctctaatc atgcccagag ggatatctgc tttataagcc gttaatactt cttcaagcct
     2641 ggcttcagtg agctgttccc atgccattat cgggcgcgtt atggtttcat tttcgggtaa
     2701 taacgttacg gcaccaacgc tgtctcgccc tatttctgac aataaatcaa acggttgtct
     2761 ggatttagca tgataacgtt taacgatccg atcacgtaca atcgggctat cgggtaacag
     2821 gttatcgaag aagttaaata cggcatcaga ggtgatattc cccctctgca atggcagcga
     2881 aagtgacaac ggtctggcat aacggctggc taaccactcc ggtgcatact taaaggtgtg
     2941 cgcgccattg gctaacttcg ttaactcgcc tacccgctgg ttgttcatcc aagtgacaag
     3001 tttaggcatt accactccag atcttgctgt tctgttgatt ctggcgaggc atttttcgtg
     3061 tcgcatagcg tcattgagag ttcaagcgac tgtaaaatct taaaaaaggt cgtcagcgtg
     3121 gtattgtcag ggttgttttc gaaattggaa atcgtcgcct gcttaatacc aatttttttc
     3181 gccagctcgc tctgcgtcca gccattttgc tggcgaacca gtttcattgc attcgccaat
     3241 tgcgttgggc tatagatctt ctgaaagctc atcatgtcat acgtccacag caaatttatc
     3301 cgcttaaggg gatattatca gttttatcct ttagtgagga taagtcaatc tggaaacaca
     3361 tcttatccgc tctacgggat aaacactgtg atatccgcga tcgcggataa aaaggaggat
     3421 ttaaaagtcg tttgaagtcg gcaatattaa gccgcatgca tcttcgacat gcggctattc
     3481 ccttgggtat cgccttttac gttcatctta tcggtgggcg aattatcgcc gcccagcgtc
     3541 gcgttgaacg tcatcaggcc attgtttccg gtatagttac cgttaacggt cagtacattc
     3601 cccggtttcc ccccttcgct gttcatttga accgtgccac cattctccag gttaccgccg
     3661 acagtggttt tagcgacacg cagtgtcccc cctggcataa cgttcacatc acctgcagtg
     3721 gtgacatttc ccgacatcac accgctgtta tggatattaa tgtgctgact tgccatatta
     3781 atggcagagt cggaaccgaa agcaatttca ccacccacga tatccgtcgt acccttatac
     3841 gccgcggaat ctttgttaag cgtcaggatc ccggtaccca gttttgtcac atcaccgcta
     3901 ccgctgatac tgtgcgtcat cacccagcta cgctcagtgg agatggtgag caggccatta
     3961 ttgataacgg aagcatcacc caggtttttc tgctcgttga cgctaagcgc gctgcctgta
     4021 tcaatgttga attgccccac aaagcggcta ttatcgccat ccagctctac gtctgtcctg
     4081 gcggttgcgc tcacgatacc cttcccgctt atgctattac gcagttcgcc ggtcacattt
     4141 tttagcgtta acagaccgtc attggcaata tttcctctgc ctaacccctg cgtattatcg
     4201 aggctgactt ccgcatttgg gctaatgctg gttaacgcat tgtagcgtgc cttaagcccc
     4261 tcgatagcca gtgttccccc tgtaacattc aggtttccgc cacctgtcag ttcgccctga
     4321 ctgatcccac ctttattcac cgtcagcgac ccctctttaa acaaaacagt cgaacccatt
     4381 tgcccggtga atgtctctac cgtctgggtc gatccattaa gatcgacgat ggccgcgttg
     4441 ctgatgttca gttcccgggt gttgcccagc gcgccatccg catcggtacg taatgtcccc
     4501 atctgaacgt aggttgcccc ctgatagtcg ttctgaccgt tggaaagcga aacctgtcgt
     4561 accgtattga ttgccagatc cccttcgcca ccgatttttg ccgacatatc gaccgttgcg
     4621 ccataagctc cgccatgttc ggctaacgtc agcttttgcc caccatggat gttcagcgct
     4681 ttcagcccat agttaacata caaaccatca ccaggtgcgg tcgtgaggcg gaatccataa
     4741 tcaccctcgg cgaccaccgt tccgttttgc gcaatatgta acgttttgtc cgcttccacc
     4801 tcgtcgccct gtaaatcacg taacgttaat gaaccacccg atccaataac cgtttgcgcc
     4861 ttcaccagtt gaacgccgac atggctatcg tcgtgttcca tcaaattgag cgtcgtcaga
     4921 gggttattcg ccatgggatc attccacggt ttaggcacgt cgataagcac gtcgcccatc
     4981 ccgtttacct gatagttacg gcctttccag gtgtagtcac ccgcgccgac aaccagcgta
     5041 tcgacgctga tatatccctc cgcaagcgtc gcagcaggta tatccgtatc gaacgtgagc
     5101 gtaccgccat tcatggctag tccaccaatg gattgctctc ccacttttac cgatgtggta
     5161 ttttcactgt cagactgcag catcgcgtaa gtaagcgcag cggtgttgtc gcgttccagt
     5221 gcgaaggtac tggctttcag ttgggcaaca ccggcgaatt cagtccctgc tgcatgggta
     5281 aagccaaaca ttttgtcgga ggatgacagc tgcactcgca tcaagccatc gcctttgagc
     5341 gcattgatca gcgtgaatag caatttcccc ttgaatatag tactggtgat ggaacgatga
     5401 atctgcaagc tggcatttaa caagccagct ctaaaagaag ggaaatgaga ataccaatac
     5461 tcaaaaacta acagccacgg tcatcatggt gtggctgtca atgaaactat aacccaggcg
     5521 ctttccataa cgacgtcgtc agtccatgat caaccagccc cagctgatcc tgataaactg
     5581 cctgccactt atcgcgtgaa tcctgataaa gttcatgctt ttccgggtct ggtgtgtgcg
     5641 tccgttccca acgaaccaag cgttctccgg tttctgccat tgatgaaaaa attccggcac
     5701 cgacgccagc tgcaatggca catcctaatg cagtggcttc tttgaccacc ggaatattga
     5761 cgggtaatcc cgagacatca gcgagaattt gactccataa tttccctttt gaacctccgc
     5821 cagcaaaaac cagcgaggtg ggatgaatat tcgagaaatc agcaatttgc tgtaagttac
     5881 acgctgatac aatcgccgca ttttcttcca gcgcacggaa caatgtcgct ttgttacatt
     5941 tatccgggtc aatggacaag ttaataaagg aaggcgcagc gtgataccag gttttaaagc
     6001 gcattctgtc ggagaatatc ggcattacgc cccacgaccc aggcggcacc cgactggcca
     6061 tctcttccag cagcgtatag gtgtcgatgc ctaaacgttc ggcaatcagt ttttcttcgg
     6121 cacagaaagc atcgcggaac cagcgcatgg tgagtccggt aaaaaagctt atagattcag
     6181 cttgtaccat gccaggaata acatggggat taacgcgcac gttcatttct gggtctgtca
     6241 ccggcgcggc taagtttaca acttgctgcc agaatgtgcc gccaagaacc gcggtttgtg
     6301 ccggacgtac aacgcctaac cccaggcaac caagctgcac gtcacctcct ccaacgacca
     6361 ccggagtgcc cgccttcaga ccgcagagtt ccgccgcttg tgaacttacc acacccagca
     6421 atgtgccggt ttctttgaca ggagaaagaa tatcggcacg caggccagcc atatccagca
     6481 atgcaggttt ccagtcacgg gtggttagat caataagtcc cgtggtgcca gcgttagagg
     6541 gatccaccgc aagttcgccg ctgagcatat aggccagcca gtcgctgatc atggtgatgg
     6601 ttgatgcctg acggtaaata tcggaacgat ggtgcgccag ccaaagtaat ctggggatgg
     6661 cgcttaaagc cagcgtttgc ccggtcgctc gataaacttt gttttcaaag gtattgttgt
     6721 gcagttcttt aagttcgtta acttcgcgcg ccgctctggc atccacattg gcgcaggccc
     6781 agatcggtgt tccttcattg ttatataaaa caatgccttc acgcatcgaa catgccgaaa
     6841 cagcagcgat atactccggg gctatgccgg cgttgtgcag cgcctggcgc atacactcac
     6901 acgccagttg ccagtttttg ttgagatcaa attccataga accaggaacg tccggtactg
     6961 ccagatgccg ccactccgcc tgtcccacgg ctatttgatt gccttccggg tcgaatatca
     7021 cagcccgaat acttccggtg cctgcatcca gcgccatcag gtagtacttt gattctgaag
     7081 gggtaaagag tcgagccatt agattatcct cggcttatgc gttctgtctg ttcctctata
     7141 cgttctccat cattcccggt aacaaggtca tgcaaattta actacgtaaa atcgccgctg
     7201 ctgtgtcctg atcggtaacc agtgcgttga tatagccgcc tttcattgca gcggcaatgg
     7261 cttcggcttt attttctccc cctgccacgc caacccggac gggtatggtc ttcagcgcgc
     7321 ttaaaggtaa gccaatcagt tcgttatgta ttttaatatc cgtgacaacg tcaccttttg
     7381 catcaaaaaa gtagcctaaa atgtcgccaa cagccccttt tcgaccaatc attaactgtt
     7441 cgccctggct gatatagccg gagcgaatga ttgttgcgtc gtcttgttga ctcacagccc
     7501 caatgccgac aatcgccaca tccgctgctt gcgcggctag cagaacatct ttgacgcaat
     7561 tttcattttt tagcgtacgg gcaatgtcag cggaggatgc ccgcaacgga gccggaataa
     7621 tattcacact gcacgccgcg ttaagctgcc cgatacccgt catataagaa ccgactccac
     7681 cggagagcgt gaccaggcga atttgctgtg acgaaataaa accacttaag cgttgcagcg
     7741 tattcatagt tgcctcgcca aaaccaatcg ccagcatctg ttgtggttga agtaaactca
     7801 tcaacatatg cgccgcgcct atccctagtc gcccgccgac atcagcatcc gcaagccctg
     7861 ggatcacccg gacatgttgc agcgaaaact gacgacgtaa ttgtgtttca tattccagac
     7921 agccttcaaa gcgagaatta atctgtacgc gaataatgcc ggattgatgc cctttctcca
     7981 gtaatcgcga cactttcaaa cgggtcaggc cgagacgatc gctgatctca ctctgggtca
     8041 gcccgtcgtg gtagtaaaac cacgcaatcc gcgcgacctg ttcttcttca cacattccct
     8101 gttctgaaat taccgaatcg ttgattgtca taattcattc ttcactttga acatatttaa
     8161 atctttaatg caattgttca gttcttgcgc atttatatct gtgatagcaa ccacagtttg
     8221 actctacgag catgaacaaa cgcaaccgtg aaaatcaaaa tagcataaat tgtgatctat
     8281 tcgtcggaaa tatgtgcaat gtccacctaa ggttatgaac aaattaaaag cagaaataca
     8341 tttgttcaaa actcacctgc aaaactgaac gggggaaata tgcaaacgag tgatacccgc
     8401 gcgttaccgc tactttgcgc ccgctcggtt tataaacagt attcaggggt taatgtcctg
     8461 aaaggcatcg attttacgtt gcatcagggg gaggtccacg ccctgctcgg cggcaatggt
     8521 gccggtaaat cgacgttaat gaagattatt gccggtatta cccctgctga tagcggtacg
     8581 ctggagattg ggggcaataa ttacaccaga ttaacgccag ttcatgctca tcagctgggc
     8641 atttatctcg ttccccagga accgctgctt tttccaagcc tgtcgataaa agaaaacatc
     8701 ctgtttggtc tggcaaaaaa acaactctcc atacagaaaa tgaataactt gctggtggcg
     8761 ctgggctgcc agtttgatct gcatagtctg gcaggatcgc tggatgtcgc cgatcgccaa
     8821 atggtggaaa tcctccgcgg cctgatgcgc gactcgcgga ttctgatcct cgatgaacct
     8881 acagcctcgc ttacccctgc ggaaaccgaa cgtttgtttc gtcgcttgca agagctgctt
     8941 gctactggcg tgggtattgt ttttatctcg cataagctgc cggaaattcg ccagattgcc
     9001 gatcgaatta gcgtgatgcg cgacggaacc atcgccttaa gcggcaaaac cagcgaactg
     9061 tctaccgacg acattattca ggccatcacg ccagcgctac gggaaaaatc gctctctgcc
     9121 agccaaaaat tatggctgga attacctggc aaccgcccac aacatgccgc gggaacgccg
     9181 gtgctgacac tggaaaatct gaccggcgaa ggtttcagga atgtcagcct gacgctcaat
     9241 gccggagaaa ttctgggctt ggctgggctg gtgggagccg gacgcacaga actggccgag
     9301 acgctctatg gtctgcgtac tttgcgtggc ggacgcatta tgctgaatgg taaagagatc
     9361 aatagattat ccaccggaga tcgtttactg cgcggtctgg tttatctgcc ggaagatcgc
     9421 cagtcatccg gacttaatct cgatgcttca ctggcgtgga acgtctgcgc ccttactcat
     9481 aaccttcgtg gattctgggc gaaaaccgcg aaagataatg ccaccctgga acgatatcgc
     9541 cgggcgctga atattaaatt taaccaaccg gaacaagctg cgcggacctt atccggcggc
     9601 aaccagcaaa aaatcctgat tgccaaatgt ctggaagctt cgccgcaagt tttgattgtc
     9661 gatgagccga cgcgcggcgt ggatgtctcg gcgcgtaatg atatctacca actgttgcgc
     9721 agcatcgccg cacaaaatgt ggctgtgctg cttatctcct ctgatctgga agagatcgaa
     9781 ctgatggcag atcgcgtgta tgtgatgcat cagggcgaaa ttgcccactc tgcactgacc
     9841 ggacgcgata ttaatgtcga gaccattatg cgcgttgcct tcggagatag tcagcgtcag
     9901 gaggcgtcat gctgaaattt attcagaaca accgtgaaat cacggcactg ctggcggtgg
     9961 tgctgctgtt tgtattaccc ggttttctcg accgtcagta tttaagtgtg caaacgctga
    10021 ccatggttta tagcagcgcg caaatcctga tcctgctggc aatgggcgcg acgctggtaa
    10081 tgcttacgcg caatattgat gtttcagtgg gttcgattac cggaatgtgc gcggtgctgt
    10141 tggggatgtt actgaacgcg gggtattcac tacctgttgc ttgtgtcgcg actttactgc
    10201 ttggtttgct cgcgggattt ttcaacggtg tcctggtcgc gtggctaaag atccctgcca
    10261 ttgttgccac ccttggcacg ttagggttgt acagaggcat catgttgctg tggactggcg
    10321 gtaaatggat tgagggatta cccgccgaac tgaaacaact ctccgccccg ctgctgtttg
    10381 gcgtttccgc gattggttgg ttgacgataa ttctggtggc atttatggcc tggctgctgg
    10441 caaagacggc gtttggacgc agtttttatg tcacgggcga taatttacag ggcgctcgtc
    10501 aactgggcgt tcgtacggaa gccattcgca ttgtggcatt ttcgttgaac ggctgcatgg
    10561 cggcactggc gggaattgtg tttgcttcgc agattggttt tatccccaac cagaccggta
    10621 ccgtgctgga gatgaaagca attgcagcct gcgtgctggg cggcattagt ttgctcggcg
    10681 gttccggcgc gatcatcggt gcggtactcg gcgcatggtt cctgacgcag atcgatagtg
    10741 tactggtgct gttgcgcatt ccggcatggt ggaatgattt tatcgcgggc atggttctgc
    10801 tggcggtgct ggtgtttgat ggacgcctgc gttgtgcgct ggaacgtaat ctgcggcggc
    10861 aaaaatatgc ccgctttatg atgccaccgc catccgttaa acccgcttcg tcaggtaaaa
    10921 aacgggaggc cgcgtaatgc gtattcgcta cggttgggaa ctggctcttg ccgcactgct
    10981 cgttattgag attgttgcat ttggtgcaat taacccgcgg atgttagatc tcaatatgtt
    11041 gctgttcagc accagtgact ttatctgcat tggcattgtc gccctaccgc tgacgatggt
    11101 gattgtcagt ggcgggatcg atatttcgtt tggttcgacc atcggcctct gcgccattgc
    11161 attgggcgta ctgtttcaaa gtggtgtgcc gatgccgctg gcgattcttc tgaccttact
    11221 gctcggcgca ttgtgcgggc tgatcaacgc cggattaatt atctatacca aagttaaccc
    11281 gctggtgatt acgcttggca cgctgtatct gtttgccgga agcgccctgc tgctttccgg
    11341 gatggccgga gcgacggggt acgaaggtat tggtggattc ccgatggcgt ttacagattt
    11401 cgctaacctg gatgtgctgg ggctccccgt tccgctgatt atcttcctga tatgtctcct
    11461 cgttttctgg ctctggctgc ataaaaccca tgccggacgt aacgtgtttt taattgggca
    11521 aagcccgcgt gtggcggttt atagcgcgat tccagttaac cgcaccttgt gtgcgctcta
    11581 tgccatgacg gggctggcgt ctgcggtcgc cgctgtgctg ctggtatcgt attttggttc
    11641 agcacgttcc gatctcggtg cgtcgtttct gatgcccgcc atcaccgccg tagtgctcgg
    11701 cggtgccaat atttatggtg gttctggttc cattatcggc accgccatag cggttttatt
    11761 agtgggatat ttgcaacaag gtttgcaaat ggcaggagtg ccaaatcagg tgtccagcgc
    11821 cctttccggt gcgctactta tcgtcgttgt cgtaggtcgt tccgttagcc tgcatcgcca
    11881 gcaaattaaa gagtggctgg cgcgtcgggc caataaccca ttgccataaa ggatatcttc
    11941 atgacacttc atcgctttaa gaaaatcgcc ttacttagcg cgcttggcat tgccgtaatc
    12001 tctatgaatg tgcaggcggc agagcgtatt gcatttattc ccaaactggt tggcgtggga
    12061 ttttttacca gcggtggcaa cggcgcacaa caagcgggta aagagttggg cgttgatgtg
    12121 acctacgacg ggccgacaga acccagtgtt tctggtcagg tacagttgat taataacttc
    12181 gtcaatcagg ggtataacgc cattatcgtt tctgcggttt cgcctgatgg cttgtgtccg
    12241 gcactgaaac gcgccatgca acgtggtgtg agagtgctga cctgggactc tgatactaaa
    12301 ccggagtgcc gctcttacta cattaatcag ggaacgcccg cccagttggg aggtatgttg
    12361 gtggatatgg cggcgcgtca ggtgaataaa gacaaagcca aagtcgcgtt tttctactca
    12421 agccccaccg ttacggacca aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa
    12481 gagcatccag gctgggaaat tgtcactacg cagtttggct ataacgatgc cactaaatcg
    12541 ttacaaaccg cagaaggaat attaaaagcc tatagcgatc tcgacgccat tatcgccccc
    12601 gatgccaatg ccctgcccgc tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagtt
    12661 gcgattgtcg gattcagtac gccaaacgtg atgcgtccat atgtggaacg cggcacggtg
    12721 aaagaatttg gcctgtggga tgtggttcag caaggcaaaa tatcagttta tgtcgcggat
    12781 gcattattga aaaaaggatc aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt
    12841 caggtggaag tctcgccaaa cagcgttcag ggctatgact acgaagctga tggtaatggc
    12901 atcgtactgt taccggagcg cgtgatattc aacaaagaga atatcggcaa atacgatttc
    12961 tgatgtgcat tacttaaccg gagtacgtta tggcagattt agatgatatt aaagatggta
    13021 aagattttcg taccgatcaa ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg
    13081 cactggattg gggaatgcag tcacgcttat cgcggatatt taatccgaaa acgggtaaaa
    13141 ccgtgatgct ggcctttgat catggttatt ttcaggggcc gactaccgga cttgaacgca
    13201 ttgatataaa tatcgccccg ctgtttgaac atgccgatgt attaatgtgt acgcgcggca
    13261 ttttgcgcag cgtagtcccc ccggcgacca ataagccggt ggtactgcgg gcgtcaggtg
    13321 cgaactctat tctggcggaa ttaagtaatg aagccgtggc gttatcgatg gatgatgccg
    13381 tgcgcctgaa cagttgcgcg gtggcggcgc aggtttatat cggcagcgaa tatgaacatc
    13441 agtcgatcaa aaatattatt cagctggtcg atgccggaat gaaagcggga ataccgacca
    13501 tggccgtgac tggtgtgggc aaagatatgg tgcgcgatca gcgttatttc tcgctcgcga
    13561 ctcgaatcgc cgctgaaatg ggggcacaga ttatcaaaac ttattatgtc gataaaggtt
    13621 ttgaacggat tgttgccgga tgtccggtac ccattgttat tgctggcggt aaaaagttac
    13681 cggagcgcga ggcgcttgaa atgtgctggc aggctatcga tcagggcgct tctggtgtgg
    13741 atatggggcg taatattttc cagtctgacc atccggtggc gatgatgaaa gccgtacagg
    13801 cggtggtaca ccataacgaa acggctgatc gggcatatga actctatctg agtgaaaaac
    13861 agtaactgcg gatttaagga gaagaattat gcacgtcaca ctggttgaaa ttaacgttca
    13921 tgaagataag gttgacgagt ttatcgaagt ttttcgccag aaccacctgg gctctgtaca
    13981 ggaagaaggc aatttgcgct tcgatgtctt acaggacccg gaagtgaatt cgcgctttta
    14041 tatctacgaa gcctataaag atgaagacgc agtggcattc cataaaacca cgccccacta
    14101 caaaacctgt gtcgcgaaac tggaatcttt aatgaccggg ccgcgtaaaa aacgtctgtt
    14161 caatgggttg atgccgtgag gcgaatttat cagttttatc tacaattggg gtaacgcgct
    14221 gacgggagta aataaatgtc tgactggaac ccctctttat atctgcactt tgccgctgaa
    14281 cgatcgcgtc cggcggtgga gctgcttgcc agagtgccgc tggagaatat caaatacgtt
    14341 gccgatcttg gctgtggccc aggtaacagc accaccctgc tgcatcaacg ttggcctgcg
    14401 gccaggataa caggcatcga ctcgtctccg gcgatgattg ctgaagcgcg cagtactttg
    14461 ccagactgcc agttcgtgga agcagacatc cgcaactggc aaccggaaca ggcactcgat
    14521 ctgatttttg ctaatgcctc actgcaatgg ctgcccgacc actacgaatt gttccctcat
    14581 ctggtttctt tacttaattc gcatggtgtg ttggcagtac agatgccaga taactggcta
    14641 gagccaaccc atgtgctcat gcgcgaagtc gcctgggaac aaaattatcc ggatcgcggg
    14701 cgtgagccgc tggctggcgt tcatgcttac tacgatattc tgagtgaagc cggatgtgag
    14761 gtcgatatct ggcgaaccac ttactatcac cagatgccgt cgcaccagtc gattatcgat
    14821 tgggtgaccg ccactggatt acgtccgtgg ttacaggatc tgaccgagag cgaacagcag
    14881 cattttctta cgcgctacca tcagatgctg gaagagcaat atcccctcca ggagaacgga
    14941 cagatattgc tggcatttcc gcgtctgttt attgttgccc gccgtacgga gtaattatca
    15001 cgtcagctgg taatggcgat cgggaagaat tttcgctgga atttctgctt catcgttcat
    15061 ctgtaacagg tcaatttcaa tagcgttgca gatggcatcc agcggtaagt cattgttttc
    15121 agtaccgaag ggatcttcca gttcttccgc cagacaatcc agcgaaataa aagtgtagga
    15181 aatcagcaca gagataaaag gcgtcatgta atgcaggtcc acgaccagcg caaacggcag
    15241 catgatacaa aacagataca cggtgcgatg caaaatcagg gtgtaggcaa agggaattgg
    15301 cgtataggca atgcgctcgc atcctgccag gaccactgaa atatcattaa gccgatcgtt
    15361 gaggctaata aacaggatat ctgaaagttg tccattgcgt cgctgaaccg ccaaccactc
    15421 ccccattatt aacaagatgc ggttagctgg agagttagaa gccagtacac gctgaagatc
    15481 ttcagtcttg agataatgag ccagcacttc cgcctgtggc tgtttgcgta atgtcatgcg
    15541 taaacagtgg gcgaaggcga tttgcagccg ggcaaactcc cttacacttg ccgaatccgg
    15601 caatgtcgtt tttacctcgc gcagtaacga ccgcgaggca atcatcaact gcccccaaag
    15661 ttttcgcgct tcaacgtaac gagcgtaacc ggcattatta cgaaaaccaa gaaaaatggc
    15721 gatggcgaca ccgagaatgc tgaacggtgc gagggtgaat ttaatgccca aatgcgtgta
    15781 ccagggcagc atgaaaataa cagcgataga aaagagaaaa ttgagtagta agcgcgagga
    15841 tatcttggat aatactgaac cgtgccagac aaaaatacgg cgcagccagt gttgttgtgg
    15901 acgaacaatc atggttatct tcaggcgtgg aaaagtcgcc ctattaaacg tgattacgat
    15961 cacattctca agacactact tacaaattac ctacaagctt acaacattaa caggtgctgc
    16021 atgtggcacc tgttaatgat aaaggcgcat aggatgttgt aactaatata gtgaaataag
    16081 aaacccggtc gaaaccgggt tcagagtatt agcacaacgg acgtacagct tcgcgcattc
    16141 cttttttgag aatcgcatcc aggtcattag ccacctgctc gaccagacca ggcacttgcg
    16201 tcaggtcctg ctcccagtgg tctttttctg ccaacacaat cgctaccagt tcctgggtac
    16261 cgataacacg gtcacgatgc tggctccaga gttgctgata acgttccagc cagtgtgcat
    16321 cgtcctgtac cggatatgtt tctccattac gctcaccgcg ataaaacgca atcaatgccg
    16381 ctaatgcgaa agtcaggcgc gccggaagtg tgccgtttgc cttctgccct gccagcagct
    16441 gcggcaggat acgggtgcgg aacttggtca taccgttaag cgcgatagac agcagctgat
    16501 gcttgatgta cggattacgg aaacgtccgg tgactgcact ggcgaaagat tccagttcat
    16561 cacgaggcaa atccagtacc ggaataatct cttcgtagat ggctttttca acgaatgcgc
    16621 aaatttcagc atcgttcatc gcttcaccta cggtatccag tcctgcctgg aacgccaccg
    16681 gcaccagccc ggtgtgcgca ccgttgagga ttgccacttt gcgctcttta tacggtttaa
    16741 tatcgtcaac aatcagcacg ttgagtggat atttgtccag gcgcagctca gtcgctaagg
    16801 atttcggccc ctgaatcaca aacaggtaaa agtgttcagc ggtgtcgagg aaaccatcgt
    16861 gataacccag ttcttcttcc agtttagcca cttcatcgcg cggataaccg gtaacgatac
    16921 ggtccaccag cgtagaacag aagctgttag cctgatccag ccattgaata aatgcttctg
    16981 gcagtgccca ctcttgcgca tagcgcagaa ccagttcacg caacgcgtcg ccattgtagt
    17041 cgatcaactc acacgggatg atgatccaac ctttatccag cgcaccgttg aaatggctaa
    17101 aacgttcgaa cagcagacgg gtcagttttg ccggataact taccgctggc gcgtcatcga
    17161 atttatcgcc cgcatggtag ctgatacccg cttcggtggt gttagagaaa acaaagcgca
    17221 tttccgggtt gtgcgccagt ttcaggaatt catcgtattc actgtagacg ctgatttcac
    17281 gattaacaga gcgaatcaga cgcgcgtcgc tgaccgcttc ccccttctca ttcaggccac
    17341 ggataatggt ggtgtacaga ccatcctgtg tgctcagtga cggtgggaat gaagtttcaa
    17401 tcggacgaac aacgaccacg ccagaattca gatcggtgtg ctcattcagg agatcaattt
    17461 gccagtcaac aaaggcgcgc aggaagttac cttcaccaaa ctgaatgata cgttctggat
    17521 actgtgcacc gggaaaatcg cgacgattta gtgttttcac aatgggttcc cttctgatta
    17581 gtcatacaac ctgttttaat tggtacgaca ggttagcaaa ctttaatacg ccgaacccct
    17641 gttttgatca actcctgatg attaatgagc agttttatga gaaaagtgtg gcgcggatca
    17701 tggtttaatc gaggaactac gccttttatt cgctcaataa agtggtagaa cacattccgt
    17761 taaaatcgct gtgaatgaat aaagatgtca gacaacttcc tcaccgtaac gcatagtgct
    17821 ggtacggttg cgcccatctt tcttcgagcg atacaaacat gtatcggctt cgaccatcaa
    17881 tttattaaag tcatcggtca gcgttcggta ggatgcgcaa ccactaccga cgccaatact
    17941 taccgtgaga taaagcgttt tttgttgcca ggtgaacggt tgcagttcaa cgcctttacg
    18001 gattttttcc gccattagca gaccatcgac aggattcacc gatggcactg caacagcaaa
    18061 ttcttcgccg cccattcgcg ccaccagccc cttatcaccg acaatcttct gaatatgctg
    18121 ggcaaacacg cttaacactt tatcgccaca ttcatgtcca tagttatcgt taatactttt
    18181 gaaatagtcg atatcaagca gcatgacagt cagatgttgt gtctgtttca gcgatggact
    18241 tttcagcgcc tcataaagac cggaccgtga gtaaacctga gtcagaaaat caaagtcggc
    18301 tcgcagcgca acttgcttca ttagcgaatt gatcgctgcc acgctaaaag aaaccataat
    18361 tgggcatatc gccatcgtgg caataccgag acgtgcggag aacatttgcg gaatggagaa
    18421 cggcgaaccg accgagatat caatcaccga atttgccacc agtacgattt ccaccgcacc
    18481 ggtgacaaag gtcaacagac atgttacctg cggcgtatag cgcactgcac accagattaa
    18541 tgcaggcagc gggaacgcca gactccccgc tccgccaatg actaccgagg caataaccga
    18601 cacaataagc gcgatagcgg gcatcatctg ttctgcttta aagcgcggca gcactccagg
    18661 aattgccaac gtcagcatac aaggcacgat caacacgccc gttgagaatt gctcactgaa
    18721 ccagtccgca agcaaaggcc agaaatccag actatcaata ctgaccgaac caatcgcccc
    18781 gacaatagcg cataataatg cggcaatcag acagtaatta aatagccgta aggcacttac
    18841 tggctcatac ttattttttc ccagacgctt atcccgcgcg accagtaagg cgacggtaac
    18901 aataaacatc atattggaga aattgatagc cagtgaaacc agcccccatt cggtggttat
    18961 ggcatcataa accagcatcg ccacataact gatcgcatag taatgcagtc gattaagcca
    19021 gacatagcgg gcaaacaccc ccgccattac gccattcagc ggccagaaaa gagaaagttc
    19081 gtgaaccagc cgtaactctg caccaataaa atagaacagc gtggttagca caaaaatagc
    19141 gatgctatta cgtagcagat gaccttcctg gaacaaacgg aaagttgaaa tgggctgtac
    19201 atgcattaat tatctgtcca tacaattgcg ggtggcaatt atctcaacgc aataagtaaa
    19261 ttcttagaaa ggatagcatg ttcgctgaca acggcggcaa ctaaagatat cctgggaatg
    19321 ctattttatc gatttccgta attatcatgg ctgattcatc tgtataacca gccagtggta
    19381 gattaccgtc acaatataac gttgttgctc ctcacttaac gcttccccct gaggaatgtt
    19441 atgccactta gccagacaac cgcgacaaca ggtcgcggtg gcgtgttgag cgataaaaac
    19501 cggatgcccg cgcatggggg tttgcttacc gtcgttagcc ggtaatgccg gagcaagacg
    19561 tttagcgaca aaatccgccg catgttgttc aatgactggc gcgcctttct ccagacaata
    19621 ctgacgctct ttcacaccca aacgaaagcg agagcgaaat tttgaacggg ataaacgcgc
    19681 gaagagtgga tcgagagact gcattaataa actgaacgcc ctaattgttt cgtcaattgt
    19741 tcaaggacgg caatgcccgc aagcgagtta ccggcatcat ccagttccgg actccagact
    19801 gcgatggcca tttcatgcgg aacaatcgcc acaataccgc caccaacgcc agattttgcc
    19861 ggtagtccca cccgccaggc aaactccccc gcgttctggt acataccgct ggtcgccatc
    19921 agcgcgttaa tttgccgcgc ctgcattggc gtcaccacag gttcatcaat atgaatagct
    19981 ttcccctgat tcgccagaaa gacaaacgtc cgggccagct ctatacagct cattttcaga
    20041 gcgcagtaat gaaagtagtt ttgcagaacg gttgtcacgt catgatggaa attgccaaac
    20101 gacttcatca gccaggcgat agccgcattt cgcgcggaat gttcaaattc ggaacgcgct
    20161 accaccgtat cgtaggaaat atcagacaca ccacttaagc cgcgcacgac ttccagcata
    20221 cgttgccgag gtgcgcttaa tcgcccttgc aacatatcgc agaccaccag cgcaccggca
    20281 ttaataaacg gattacgcgg aataccctgc tccatttcca gttgcactaa agaattgaac
    20341 ggtgatccag acggatcttt gccgacgcgt tgccagattt cctcttcgga gtaatgacgc
    20401 atggcgacaa cgagactcag cactttggaa atagactgaa tggaaaaacg ttcttgcgcg
    20461 tctccggcct gaaaaagctg tccgtcaacg gtacagatag caatccccat tcgggaaccg
    20521 tctaccgtag ccagcgccgg aatataatcc gcgactttac cctgaccaat gagcggccgc
    20581 acttgccgca agatgttttc taaaattgca ttatccatgg cgactgccac tttctactcc
    20641 tggaccgcag gtctgaaaag acctgcgagt atatcagagc tgaatatgta gcgtcagatc
    20701 cggtctttcc acaccgtctg gatattacag aattcgtgta agccaaaatg ggaaagctca
    20761 cgcccaaagc cactcttctt cacgccacca aaagcgactc gcgcgtcgct ggcacagtaa
    20821 ccattgataa acaccccacc gcattccaga cgtgccgcca tctgtctggc ccgggtttcg
    20881 tcggtggtga aaatggtcgc tgaaaggccg aactcgctat cattagccag ttccagtgca
    20941 tgttctgcat ctttcgcaat ggtgattgcc gcaacagggc caaacatttc ttcccgaaac
    21001 gcggtcattt ctggggtaac gttcgccaga accgttggcg gatagtagtt acctgcccca
    21061 gccatctttt ccccgcccag taacaaacgc gcaccctgcg ccagggtttt ttccacctga
    21121 tgatgcagct catcacgtaa atcaaaacga gccattggtc cgagagcgtt ctcttcgtca
    21181 cggggatcgc ccattttcaa ggctgccgca gctgccacaa aacgttcggt aaatgccgaa
    21241 gcaattccct cttcgataat aaagcgtttt gccgccgcac aaacctgtcc ggtattctga
    21301 taacgtccgg ctaccgccgc tttaaccgcc agttccagat cggcatcgtt aagcacaata
    21361 aatggatccg aaccgcccag ttccagtacg cattttttca gtgccgctcc agcctgtgcg
    21421 ccaatagccg ctcccgcacg aacacttccg gtcactgtga cagcagcaat gcgcgaatct
    21481 ttaatcatct gactgacacc gttgttgtca gcattcagcc agccatatac gccttgcggg
    21541 atacccgcat ctttaaacac ctgggcaatg agctgtgcac agcccatcac attcggcgca
    21601 tgtttaagta agtagccgtt acctgcaaga atgatgggaa ccgcgccacg catcacctgc
    21661 cataacggaa aattccacgg cataatcgcc agaattgtcc ccaacggtcg atactcaata
    21721 actgcctgct gattttccac cagcgtaggt tccgccttca gcattgccgg accatgttct
    21781 gcataccagt cacacaaatt cgccgatttc gccacttcag cgcgcgcctg gttgattggt
    21841 ttgcccattt cacgggtgat catttgcgcc atttcttcgc tacggacacg cagagcctta
    21901 ccgatatcac gcagtttttc agcacgataa tctatatttg tctcgcgcca gtcgcgaaag
    21961 cctgctgccg ccagctgaag tgcgttttcg atatcatcag cgccagccca cggcagcaca
    22021 gaaagttgtt caccggtggc aggatttatc gaaattgcat gagttgccgg agtaatggtc
    22081 atcggggtat ctcctttatg agttatggta aaaagatacg cagatttact cttgctttaa
    22141 aatgaataat attaagccac ttattcacga atcgagaata ttatggatct gacccaactg
    22201 gagatgttca acgcggttgc cgaagctggc agcattacgc aggctgcaac aaaagtgcat
    22261 cgcgtgccgt cgaatctcac tacccgtcta cgccagctgg aaacagaact gggggttgat
    22321 ctgtttattc gcgagaatca gcgtttacgt ctctcgcctg ccgggcataa ctttttacgc
    22381 tatagccaac aaattctcgc gttagtggat gaagcgcgga gcgttgtcgc tggcgatgag
    22441 ccgcaaggtt tattttctct tggttcgctg gaaagcaccg cagcagtgcg cattccggcc
    22501 atgctggcgg aatttaaccg tcgttatccc aaaattcagt tttcactttc caccggccct
    22561 tccggcacta tgctggaagg ggtgctggag ggaaaactga atgcggcgtt tattgatggg
    22621 cccattaacc atactgccat tgacgggata ccggtgtacc gcgaggagct gatgattgtc
    22681 acgccacaag gacatgcgcc agtaacccgt gccagtcagg ttaatggcag taacatttat
    22741 gccttccgcg ccaattgttc gtatcgtcgc cacttcgaga gctggtttca tgctgacggt
    22801 gccgctccgg gaactattca cgagatggag tcgtatcacg ggatgttggc ctgtgtggtc
    22861 gcaggagcag gcattgcgct tattccacgc tcaatgctgg aaagtatgcc ggggcatcac
    22921 caggttgaag cgtggccgtt agctgagcaa tggcgttggt taacaacctg gctggtctgg
    22981 cgtcgcggtg cgaaaacgcg tccgcttgag gcatttattc aactactgga tgtgtctgac
    23041 tcggcaaaac agagttagta atgagctatt tttgatagtt cttgcggcta atatgctcta
    23101 tatagtgatg ctccgatgac ttatgactat atggggcaaa tatggttacg ccagtaagca
    23161 tcagcaattt catctcactt cctgatgatt ttcccgttcg taatattgca cctcaggtaa
    23221 aagaagaaca ggcccccttc ctgatcgtca ttaaaatgtg atgttaacaa ctctctgatg
    23281 cgtgacagaa caaccgtcag agagatggaa atgcgatcca gatcacaaat gcattgtatt
    23341 cacatcatta accgttttaa gatcatttca tcactttttc gcaactcacc cgataatctg
    23401 ttatgacaac aaacactgtt tcccgcaaag tggcgtggct acgggtcgtt acgctggcag
    23461 tcgccgcctt catcttcaac accaccgaat ttgtcccagt tggcctgctc tctgacattg
    23521 cgcagagttt tcacatgcaa accgctcagg tcggcattat gttgaccatt tacgcatggg
    23581 tagtagcgct aatgtcattg ccttttatgt taatgaccag ccaggttgaa cggcgcaaat
    23641 tactgatctg cctgtttgtg gtgtttattg ccagccacgt actgtcgttt ttatcgtgga
    23701 gttttaccgt tctggtgatc agtcgcattg gtgtggcttt tgcacatgcg attttctggt
    23761 cgattacggc gtctctggcg atccgtatgg ctccggccgg gaagcgagca caggcattga
    23821 gtttaattgc caccggtact gcactggcga tggtcttagg tttacctctc gggcgcattg
    23881 tggggcagta tttcggttgg cgaatgacct tcttcgcgat tggtatcggg gagcttatca
    23941 cccttttgtg cctgattaag ttacttccct tactgcccag tgagcattcc ggttcgctga
    24001 aaagcctccc gctattattc cgccgcccag cattgatgag catttatttg ttaactgtgg
    24061 tggttgtcac cgcccattac acggcataca gctatatcga gccttttgtg caaaacattg
    24121 cgggattcag cgccaacttt gccacggcat tactgttatt actcggtggt gcgggcatta
    24181 ttggcagcgt gattttcggt aaactgggta atcagtatgc gtctgcgttg gtaagtacgg
    24241 cgattgcgct gttgctggtg tgcctggcac tgctgctacc tgcggcgaac agtgaaatac
    24301 acctcggggt gctgagtatt ttctggggga tagcgatgat gatcatcggg cttggtatgc
    24361 aggttaaagt gctggcgctg gcaccagatg ctaccgacgt cgcgatggcg ctattctccg
    24421 ggatatttaa tattggaatc ggcgcgggtg cgttggtagg taatcaggta agtctgcact
    24481 ggtcaatgtc gatgattggt tatgtgggcg cggtgcctgc ctttgccgcg ttaatatggt
    24541 caatcatcat tttccgccgc tggccagtga cactcgaaga acagacgcaa tagttgaaag
    24601 gcccatttgg gcctttttta gtggtacgtt ttaatgattt ccaggatgcc gttaataata
    24661 aactgcaccc ccatacatac cagcaggaat cccatcagac gggagatcgc ttcaatgccc
    24721 cccttaccca ccagccgcat aattgcgcca gagctgcgta gacttcccca caaaataacc
    24781 gccaccagga aaaagatcag cggcggcgca accatcagta cccaatcagc aaaggttgaa
    24841 ctctgacgca ctgtggacgc cgagctaata atcatcgcta tggttcccgg accggcggta
    24901 cttggcattg ccagtggtac aaaagcaata ttggcgctgg gttcatcttc cagctcttcc
    24961 gacttgcttt tcgcttctgg tgaatcaatc gctttctgtt gcggaaagag catccgaaaa
    25021 ccgataaacg cgacgattaa gccgcctgca attcgcagac cgggaatcga aatgccaaat
    25081 gtatccatca ccagttgccc ggcgtaatac gccaccatca tgatggcaaa gacgtacacc
    25141 gaggccatca acgactgacg attacgttcg gcactgttca tgttgcctgc caggccaaga
    25201 aataacgcga cagttgttaa tgggttagct aacggtagta acaccaccag ccccaggcca
    25261 attgctttaa ataaatctaa cattggtggt tgttatcctg tgtatctggt ttatcagcga
    25321 aaagtataag gggtaaacaa cgataaagtg tcactcttta gctaaccttg catcgcattg
    25381 accaaaactt gaaccgattt agcaaaacgt ggcatcggtc aattcattca tttgacttat
    25441 acttgcctgg gcaatattat cccttgcaac taattacttg ccagggcaac taatgtgaaa
    25501 agtaccagcg atctgttcaa tgaaattatt ccattgggtc gcttaatcca tatggttaat
    25561 cagaagaaag atcgcctgct taacgagtat ctgtctccgc tggatattac cgcggcacag
    25621 tttaaggtgc tctgctctat ccgctgcgcg gcgtgtatta ctccggttga actgaaaaag
    25681 gtattgtcgg tcgacctggg ggcgctgacc cgtatgctgg atcgcctggt ctgtaaaggc
    25741 tgggtggaaa ggttgccgaa cccgaatgac aagcgcggcg tactggtaaa acttaccacc
    25801 agcggcgcgg caatatgtga acaatgccat caattagttg gccaggacct gcatcaagaa
    25861 ttaacaaaaa acctgacggc ggacgaagtg gcaacacttg agcatttgct taagaaagtc
    25921 ctgccgtaaa caaaaaaaga ggtatgacga tgtccagacg caatactgac gctattacca
    25981 ttcatagcat tttggactgg atcgaggaca acctggaatc gccactgtca ctggagaaag
    26041 tgtcagagcg ttcgggttac tccaaatggc acctgcaacg gatgtttaaa aaagaaaccg
    26101 gtcattcatt aggccaatac atccgcagcc gtaagatgac ggaaatcgcg caaaagctga
    26161 aggaaagtaa cgagccgata ctctatctgg cagaacgata tggcttcgag tcgcaacaaa
    26221 ctctgacccg aaccttcaaa aattactttg atgttccgcc acataaatac cggatgacca
    26281 atatgcaggg tgaatcgcgc tttttacatc cattaaatca ttacaacaac tagttgataa
    26341 cgtgacaacg tcactgaggc aatcatgaaa ccacttttat ccgcaatagc aactgcgctt
    26401 attctctttt ctgcgcaggg cgttgcggaa caaaccacgc agccggttgt tacttcctgt
    26461 ggcaatgtcg tggttgttcc cacatcgcag gaacaaccac cgtttgattt aaatcacatg
    26521 ggtactggca gtgataagtc ggatgcgctc ggcgtgccct attataatca acacgctatg
    26581 tagtttgttc tggccccgac atatcggggc ttattaactt cccactttta ccgctttacg
    26641 ccaccgcaag ccaaatacat tgatatacag cccggtcata atgagcaccg caccgaaaaa
    26701 ttgtagaccc gttaagcgtt catccaataa tagtgccgca cttgccagtc ctactacggg
    26761 caccagtaac gataacggtg caacccgcca ggtttcatag cgtcccagta acgtccccca
    26821 gatcccataa ccaacaattg tcgccacaaa cgccagatac atcagagaca agatggtggt
    26881 catatcgata gtaaccagac tgtgaatcat ggttgcggaa ccatcgagaa tcagcgaggc
    26941 aacaaagaag ggaatgattg ggattaatgc gctccagatt accagcgaca tcaccgccgg
    27001 gcgcgttgag tgcgacatga tctttttatt gaagatgttg ccacacgccc aactaaatgc
    27061 tgccgccagg gtcaacataa agccgagcat cgccacatgc tgaccgttca gactatcttc
    27121 gattaacacc agtacgccaa aaatcgctaa ggcgatcccc gccaattgtt tgccatgcag
    27181 tcgctccccg aaagtaaacg cgccaagcac gatagtaaaa aacgcctgtg cctgtaacac
    27241 cagcgaagcc agtccagcag gcataccaaa gttaatggcg caaaacagaa aggcaaactg
    27301 cgcaaaactg atggttaatc cataccccag cagcaaattc agtggtactt tcggtcgagc
    27361 gacaaaaaag atggccggaa aagcgaccag cataaagcgc aaaccggcca gcatcagcgg
    27421 tggcatgtta tgaagcccca ctttgatgac cacaaaattc agcccccata cgaccactac
    27481 cagtagcgcc aacaccccat cttttcgcga cattataccg cctctgaatt tcatcttttg
    27541 taagcaatca acttagctga atttactttt ctttaacagt cgattcgtta gtcaccgttt
    27601 acgacggcat taatgcgcaa ataagtcgct atacttcgga tttttgccat gctatttctt
    27661 tacatctcta aaacaaaaca taacgaaacg cactgccgga cagacaaatg aacttatccc
    27721 tacgacgctc taccagcgcc cttcttgcct cgtcgttgtt attaaccatt ggacgcggcg
    27781 ctacgctgcc gtttatgacc atttacttga gtcgccagta caacctgagt gtcgatctaa
    27841 tcggttatgc gatgacaatt gcgctcacta ttggcgtcgt ttttagcctc ggttttggta
    27901 tcctggcgga taagttcgac aagaaacgct atatgttact ggcaattacc gccttcgcca
    27961 gcggttttat tgccattcct ttagtgaata acgtgacgct ggtggtgctc ttttttgccc
    28021 tcattaactg cgcctattcc gtttttgcta ccgtgttgaa agcctggttt gccgacaatc
    28081 tttcgtccac cagcaaaacg aaaatcttct caatcaacta caccatgctc aacattggct
    28141 ggaccatcgg tccgccgctc ggcacgttgt tggtgatgca gagcatcaat ctgccctttt
    28201 ggctggcagc tatctgttcc gcgtttccca tgcttttcat tcaaatttgg gtaaagcgca
    28261 acgagaaaat catcgccacg gaaacaggca gtgcctggtc gccgaaagtt ttattacaag
    28321 ataaagcact gttgtggttt acctgctctg gttttctggc ttcctttgtt agcggcgcat
    28381 ttgcctcgtg catttcacaa tatgtgatgg tgattgctga tggtgatttt gccgaaaagg
    28441 tggttgcggt tgttcttccg gttaatgctg ccatggtagt tacgttgcaa tattccgtgg
    28501 gccgccgact taacccggct aacatccgcg cgctgatgac agcaggcacc ctctgtttcg
    28561 tcattgggct ggtcggtttt attttttccg gcaacagcct gctattgtgg ggtatgtcag
    28621 ctgcggtatt tactgtcggt gaaatcattt atgcgccggg cgagtatatg ttgattgacc
    28681 atattgcgcc gccaggaatg aaagccagct atttttccgc tcagtcttta ggctggcttg
    28741 gtgcggcgat taacccatta gtgagtggca tagtgcttac cagtctgccg cctttctcgc
    28801 tgtttatcat cttagcgttg gtgatcattg ctgcgtgggt gctgatgtta aaaggaattc
    28861 gtgcaagacc gtgggggcag cccgcgcttt gttgatttaa gtcgaacaca ataaaaattt
    28921 aattcagcct tcgtttaggt tacctctgct aatatctttc tcattgagat gaaaattaag
    28981 gtaagcgagg aaacacacca caccataaac ggaggcaaat aatgctgggt aatatgaatg
    29041 tttttatggc cgtactggga ataattttat tttctggttt tctggccgcg tatttcagcc
    29101 acaaatggga tgactaatga acggagataa tccctcacct aaccggcccc ttgttacaac
    29161 tgtgtacaag gggcctgatt tttatgacgg cgaaaaaaaa ccgccagtaa accggcggtg
    29221 aatgcttgca tggatagatt tgtgttttgc ttttacgcta acaggcattt tcctgcactg
    29281 ataacggatc gttgacacag tagcatcagt tttctcaatg aatgttaaac ggagcttaaa
    29341 ctcggttaat cacgttttgt tcgtcaataa acatacagcg atttcttccg gtttgcttac
    29401 cctcatacat tgcccggtcc gctcttccaa tgaccacatc cagaggctct tcaggaaatg
    29461 cgcgactcac acctgctgtc acggtaatgt tgatatgccc ttcagaatgt gtgatggcat
    29521 ggttatcgac taactggcaa attctgacac ctacacgaca tgcttcttca tcattagccg
    29581 ctttgacaat aatgataaat tcttcgccac cgtagcgata aaccgtttcg taatcgcgcg
    29641 tccaactggc taagtaagtt gccagggtgc gtaatactac atcgccgatt aaatgcccgt
    29701 aggtatcatt aaccaattta aatcggtcaa tatccaacaa cattaaataa agattcagag
    29761 gctcagcgtt gcgtaactga tgatcaaagg attcatcaag aacccgacga cccggcaatc
    29821 ccgtcaaaac atccatattg ctacggatcg tcagcaaata aattttgtaa tcggttaatg
    29881 ccgcagtaaa agaaagcaac ccttcctgaa aggcgtcgaa atgcgcgtcc tgccagtgat
    29941 tttcaacaat agccagcatt aattcccgac cacagttatg catatgttga tgggcagaat
    30001 ccattagccg aacgtaaggt aattcatcgt tatcgagtgg tcccagatga tcaatccacc
    30061 gaccaaactg gcacagttca taagaatggt tatccgttat ttctggctta ctggcatctc
    30121 tcgcgaccac gctgtgaaac atacttacca gccactggta gtgggcatcg atagccttat
    30181 tgagatttaa caagatggca tcaatttccg ttgtcttctt gatcattgcc actccttttt
    30241 cacagttcct tgtgcgcgct attctaacga gagaaaagca aaattacgtc aatattttca
    30301 tagaaatccg aagttatgag tcatctctga gataacattg tgatttaaaa caaaatcagc
    30361 ggataaaaaa gtgtttaatt ctgtaaatta cctctgcatt atcgtaaata aaaggatgac
    30421 aaatagcata acccaatacc ctaatggccc agtagtttag gccatcaggc taatttattt
    30481 ttatttctgc aaatgagtga cccgaacgac ggccggcgcg cttttcttat ccagactgcc
    30541 gctaatgttg atcatctggt ccggctgaac ttctcgtcca tcaaagactg ccgcaggaat
    30601 aacaacatta atttcaccac tcttatcgcg aaaaacgtaa cggtcctctc ctttgtgaga
    30661 aatcaaatta ccgcgtagtg aaaccgaagc gccatcgtgc atgttttttg cgaaatcaac
    30721 ggtcattttt tttgcatcat cggttccgcg atagccatct tctattgcat gaggcggcgg
    30781 tggcgctgca tcctgtttta atccgccctg gtcatctgcc aacgcataag gcatgacaag
    30841 aaaacttgct aatacaatgg cctgaaattt catactgact ccttaattgc gtttggtttg
    30901 acttattaag tctggttgct atttttataa ttgacaaata agaatactgc caattgttat
    30961 aaggc
//
LOCUS       DAZKGF010000036        30913 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZKGF010000036 DAZKGF010000000
VERSION     DAZKGF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.036"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            94..303
                     /locus_tag="ACHOQV_001628"
     CDS             94..303
                     /locus_tag="ACHOQV_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630020.1"
                     /translation="MNITRNSQLKERTMNDTRRISDRELTKMIESARQLLSRQNPVVE
                     ETWWENLLAALTELRERRNNEGAAK"
     gene            300..647
                     /locus_tag="ACHOQV_001629"
     CDS             300..647
                     /locus_tag="ACHOQV_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9630021.1"
                     /translation="MKTPGMTGKEIILRYLETHTRFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEKERQELAQRAERQQEKSVIEACKRSEIMKRILFI
                     YGAGRDLPVISAQ"
     gene            723..929
                     /locus_tag="ACHOQV_001630"
     CDS             723..929
                     /locus_tag="ACHOQV_001630"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9630022.1"
                     /translation="MAQNSQPQCGFTLSHSGSSTKNWQNTKKGDLVQVHGNLQVNRYQ
                     DKFGQQKEGWQLLAQSIISSRTAR"
     gene            1049..1423
                     /locus_tag="ACHOQV_001631"
     CDS             1049..1423
                     /locus_tag="ACHOQV_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015834182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HFP9630023.1"
                     /translation="MKHNDHGLNTLLDMHGYQHHMDNGYWWKIEAYRVSPNQFRPHGI
                     RYNLTLHDPYNSRVFGMDNAHGIKPPKKGKFTGRITVYDHIHRTSSDKGYPYEFTSAE
                     QLLLDFFNHVDRVIAEQQRGKK"
     gene            1420..1797
                     /locus_tag="ACHOQV_001632"
     CDS             1420..1797
                     /locus_tag="ACHOQV_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9630024.1"
                     /translation="MKARIGIIPEKVLRQRMLDIAKGKLKPEPDEPRVWFSSLNALGQ
                     ALSNENIALLRIMDAEKPQTMTELASLSGRQLSNLSATLKMLSRYGFVSLEKQGNCVH
                     PKALFTDFEIIIDPSVGETHRAA"
     gene            complement(2783..3829)
                     /locus_tag="ACHOQV_001633"
     CDS             complement(2783..3829)
                     /locus_tag="ACHOQV_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9630025.1"
                     /translation="MSKRKNYIGKRGQHSLPDALKSTPALSAFTFDGPYEVSRMDLLD
                     NMSCADNGRYFDTPMDWNAIARASRRASWHQSALYFKRNALNGCFIPHSLLSRQAFSA
                     FALDWFVFGNAYLEVRRNRLGEPLLLRPALAKYTRRGSDLDTYWYLNDDGTEFSFRKG
                     AVCHVLNPDINQEIYGMPEYIGGLLSVSLSNSADTFRKLYYDNGSHAGCIIYVGTPQA
                     NAESVEAIKKTLTDSRGRGAFKNLFLHAPGGGKDGVQILPFQQITAKDEFLNIKGSAR
                     DDILAAHRVPPQLMGAMPDGNAAFGDVEKAARVFFINELQPVMEAMKHVNEWLGIEVM
                     RFNPYALLEEGSAR"
     gene            complement(3835..5568)
                     /locus_tag="ACHOQV_001634"
     CDS             complement(3835..5568)
                     /locus_tag="ACHOQV_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFP9630026.1"
                     /translation="MIQDVFIRMRAKQLYWQGYTPADIAQLMGINQNTIYSWKKRDDW
                     DATPPIQRVTQSIDARLIQLTAKSDKTGGDFKEIDLLTRQLKKLNEGQGEQAVSGKKP
                     RKRKLKNHFTEEQIIALRERTLQTLACHQREWYENRHHRNRMILKSRQIGATWYFAHE
                     ALLDALRDDVSAGYQRNQIFLSASRRQAYQFKHFIQQAALEVDVELKGGDKIVLSNGA
                     ELHFLGTSAATAQSYTGHLKFDEFFWVSNFVQLRKVAGAMATLKGLTRTYFSTPSGET
                     HEAYQFWTGDRWNSERPRSKRTEFDVSHSNLKGGVLFPDKTWRQIVTIEDAVANGWEY
                     TSIDDIRDENTESEFRNLYMCEFVRDGEAAFSLNALIGCGVDGYDEWPDWKPFAARPM
                     GVREVWLGYDANGSSGKGDCGALSVCVPPMVAGGRFRTVETVRVQGMEFEQQAALIEE
                     YTQKYNVKHIGIDVTGGNGEGVFQIVKKFFPMAVPYNFNIASKRALVMKMLQVVRAGR
                     WEYDRSERDLIQAFASVRKIQTPGGYITYDTDRSRGVSHGDLAWATMLAVINEPIGAE
                     REDNRGFAMEF"
     gene            5725..6561
                     /locus_tag="ACHOQV_001635"
     CDS             5725..6561
                     /locus_tag="ACHOQV_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP9630027.1"
                     /translation="MGSQKAPERKKFRVAVSGSTIDGREISGEMLRRAAENYDPSVYG
                     SRVNIEHALSLWPSSELCAMGDLIALSTDEITEGKLAGRTALYAEIEPTDRMKNMLDE
                     GKKVYSSIELLPDLDAVQGPYIVGLAMTDTPASLGTERLKFAAQQRASIMRFNNRTSE
                     PSMFTAAMEAELLTTAEQQTEEGRQWFSRVMSLISKARGTDSEQFSQIREVVESIASD
                     HADLLNRFSALEKKHASGQETLKKLHDELAELKTQLKTLDADPQQRFIATGGNNQILA
                     EF"
     gene            6579..7664
                     /locus_tag="ACHOQV_001636"
     CDS             6579..7664
                     /locus_tag="ACHOQV_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP9630028.1"
                     /translation="MARLSLSREGRDSLERYMLQQAQLAGITTERLSKTFSVDPSIQQ
                     KMENAIKESAELLQKINVIGVDNQEGEKVLIDTSGPIASTNATSDGVKRRNPASVADL
                     DARRYRCEQVNYDTFISYAQIDAWSTQKNFQQLLSAQITRQIALDRIMIGFNGESHAL
                     ISDRAANPLLQDVNPGWLKHIRDKADTRIVKGMTLTRRDEENKLVSKGDYGNADAMVH
                     DIRSSVLDEWHKDAPDLVVLMGRDLFNTLRLPMINAISTTNPNTELVAGKLIVASRAI
                     GGLPTYLAPFFPKDAMLITSLSNLSIYFQKGSLRRYIREEPEYNRIATYQSMNDCYVV
                     EDYGKCALIEDISFAGEDPDEKPDSEE"
     gene            7730..8521
                     /gene="gpM"
                     /locus_tag="ACHOQV_001637"
     CDS             7730..8521
                     /gene="gpM"
                     /locus_tag="ACHOQV_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFP9630029.1"
                     /translation="MLTPAQRYVQQAVARNRVTMSTRETQRERTAHEQVLHQLRLAQQ
                     QLKGVQSNIARAELKRHLLPDFEGWIEGTLAGNSGRQDEVITTMMVWAIDCRDYPLAL
                     RIGEYVIRHGLALPDNFGRDAATVLTEEIAEIVLTQAATNSDADLSGYTVMLDTLHGL
                     VHDQDMPDQVRAKLNKARAFSRRASTQPDDIHQSLMLLREAMRLNPAAGVKRDIATLE
                     RAVKKLSSPPPDPVKKSTKTTRKTGNSQKTSAKKKPSTRKPKNTH"
     gene            8631..9125
                     /locus_tag="ACHOQV_001638"
     CDS             8631..9125
                     /locus_tag="ACHOQV_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP9630030.1"
                     /translation="MSLIARTPAEHISEDIPDTDDGDSCVTADAFWPVIVLRELRLAV
                     RLPGRTTTARLMHAATEAVAHVTAELEMWKQERMKEGYTALADIPATHVNSESVHVHR
                     YRRAVYALARAFVLERTRDVDTTEKGDKKADSLDPQVEALWRDARWAISDIRGVTRIY
                     AELV"
     gene            9125..9325
                     /locus_tag="ACHOQV_001639"
     CDS             9125..9325
                     /locus_tag="ACHOQV_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFP9630031.1"
                     /translation="MKIKALEGDTVDLLCFRHYGSTQGVTEQVLEANPGLCEQVFLVA
                     GQEVELPEQIREKKQETVLLWS"
     gene            9316..9612
                     /locus_tag="ACHOQV_001640"
     CDS             9316..9612
                     /locus_tag="ACHOQV_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9630032.1"
                     /translation="MELNIFQRIYDHITFSMSLMVTGIGVMTVSEKIAVAGLLLGALS
                     GLRAWIHRARLEKAQRRRNELIAQILAQSESRPLNASEKQALKQLEDQDDAPPH"
     gene            9596..10141
                     /locus_tag="ACHOQV_001641"
     CDS             9596..10141
                     /locus_tag="ACHOQV_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9630033.1"
                     /translation="MPRHIKRYAVATIVALGVTLAPGALRTSEEAQVKIATWEDCRAR
                     PYYDTAGILTTGCGSTGNVKNRLYSENEVAERWVNDMQRAENCINKNFHGAAMPQSVF
                     EAMTDAAYNLGCTSLMWFKDRHGQRHRTTIWKHAQAHRWIQACARLTDFVNSGGKRIP
                     GLVNRRTDFKAWCLKDTGATS"
     gene            10138..10578
                     /locus_tag="ACHOQV_001642"
     CDS             10138..10578
                     /locus_tag="ACHOQV_001642"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630034.1"
                     /translation="MKPAGILFLMVLCMVAIAIAFRQTWRLGQESARNEALHETIRSN
                     SATLESIIAISRNTHALIAGLKVAEQHRNQEGEVRREHIRNAIRSDRCADTPVPAAIS
                     DSLQKRTVRLSPELVRSSTGEPHHSHNATTADGASDMGKNRSME"
     gene            10685..12583
                     /locus_tag="ACHOQV_001643"
     CDS             10685..12583
                     /locus_tag="ACHOQV_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9630035.1"
                     /translation="MKKYELITAMTCDELAKALDERLAEGWQPHGAPVAASEPGVPFY
                     LMQALIKSTDTATQGSNINTPTAEPEYYFVIPVAGQSNGMAYGEGLPLPETCDRPDPR
                     IKQLARRSTVTPGGESCRYNDIIPADHCLHDVQDMSGKNHPKADTSKGQYGTVGQGLH
                     IAKKLLPFLPSDAGILLVPCCRGGSAFTAGADGTYSDTTGASGNSARWGMDKPLYKDL
                     ISRTKAALAKNPKNRLLAVVWMQGEFDIDAKPTEHSALFLAMVEKFRTDLAEQAEQCT
                     GGSAAGVPWICGDTTYFWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENGANVPTN
                     NPAEDPDIESIGYYGSTWRNSAATWTSADRESHFSSWARRGIISDRLATAILTHTVRA
                     AAVQPGASSSEPDVPETAATTTLLSYIASESEGNLKAQGWSASGGKAEIISDTGATGG
                     KAMKLTKQTGKGSWVLEHDAGTGDSLLQKGGLIRCRFKAQGELAANRYVMALYWPVSS
                     LPQNVTLAGNAGNNLLASFYIQTDAKDMNVMYHNAKAAKDNQKLGTFGAFDNEWHTLA
                     FRFAGNNSLQVTPVIDGKDGAPFTLTQSPVGMFTADKLHVTDVTKNATYPVLIDSIAV
                     EINNPVTA"
     gene            12737..12889
                     /locus_tag="ACHOQV_001644"
     CDS             12737..12889
                     /locus_tag="ACHOQV_001644"
                     /inference="COORDINATES: protein motif:HMM:NF018789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9630036.1"
                     /translation="MDPVYTILLCFCTITCVLFLASGGGEILYQWCAKKLHEWLNKSE
                     RDTPDQ"
     gene            12911..13381
                     /locus_tag="ACHOQV_001645"
     CDS             12911..13381
                     /locus_tag="ACHOQV_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9630037.1"
                     /translation="MLKMNALRDTLADTCRWCKANPDKFTVCVESGSIETTGESPSFL
                     YRYNLVIFAMDFTQDIDELFLPLLHWLYHNQPDLLLNPEKNSSIKFSASPNNDDSVDV
                     VIELPVWERVRVTRTPDRQVRAEHLQEPRPRIPSARGAWSNVYEDVTWSIPRDE"
     gene            13374..14021
                     /locus_tag="ACHOQV_001646"
     CDS             13374..14021
                     /locus_tag="ACHOQV_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP9630038.1"
                     /translation="MNEHKLDAVFRDILAGMSAAGLSRTARQVGQAVRRSQQQRMRAQ
                     KAPDDTPWAPRKRQIQRVQQGIKFLWQPPAAGSQPMVRELKNWKHGTGRHGPHITGYD
                     INRGGLRTFYRADIEEYLEIRTRRVSQGNTVRARMFERLRRYVKILQADNESIRVGFN
                     GDQARIARVHHFGLRDNVVPGVTTQYPARELLGLSVKDKAMIRETIIGSLKGGMK"
     gene            14018..14599
                     /locus_tag="ACHOQV_001647"
     CDS             14018..14599
                     /locus_tag="ACHOQV_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009486481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP9630039.1"
                     /translation="MSAEIMRLLENMIRVGVISDVDLKNWRVRVTSGRLTTDWLRWNC
                     TRAGVFKIWLPPSRGEQVLLLCPGGNPEMAMVVGSLYSTDNPAPGTQQDEVIITARDG
                     ARLRYNAKEGALDATGIKTARVTAETRITLETPLVECTQHLKTRTFELTDGGTLKGDV
                     THSGGSLTSNGVVTHTHTHSGVQSGGSQTGGPQ"
     gene            14596..14946
                     /locus_tag="ACHOQV_001648"
     CDS             14596..14946
                     /locus_tag="ACHOQV_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFP9630040.1"
                     /translation="MTATYTGMNPYHPGVVHDTEHLHCSVRDILLTPQGSRIMRRDYG
                     SLVPDLLDAPQNDVTRLQCMSAAVIALTRHEPRLALNTIDVRWLDEGGAELLLTGIIT
                     ATMQTVRLSIRTGD"
     gene            14950..15846
                     /locus_tag="ACHOQV_001649"
     CDS             14950..15846
                     /locus_tag="ACHOQV_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFP9630041.1"
                     /translation="MPTVDLSQLPKPAIINTPDFEVILNEIKTMMIGCFPEQQQPAVT
                     AALALESEPLTIIAEVMAFREMLLRQRINEGVAACMLSHASGTDLDHLAANLNTQRLV
                     ITPATDTAPAVMENDTALRLRAQSAYEGLSVAGPSASYEYFARSASGQVADARAVSPS
                     PAVVVVSILSTEGDGTASQELIQTVSNALNDERIRPVADRLTVQSAKIVKYRINARLF
                     LYPGPESEPVILAARQSLDEWLTEQAHIGRDVARSAIMSALHVQGVQRVELEEPASNI
                     AVDDSQFARCESIIVDMGGTNE"
     gene            15839..16381
                     /locus_tag="ACHOQV_001650"
     CDS             15839..16381
                     /locus_tag="ACHOQV_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFP9630042.1"
                     /translation="MSDSLLPPSASDFLKYTGYATDRITRIPVDLRTLWDPEACPVEL
                     LPYLAWALSVERWDKNWPEETKRKVIRESWLIHRHKGTFSSLRRIVEPLGYIISMSEW
                     WETGDPPGTFRLNVGVFEGGLTEEMYFEIERLIADARPVSRHLVGLNIVQDISGKVYT
                     GGMVYDGDIITVYPIQESDA"
     gene            16378..18033
                     /locus_tag="ACHOQV_001651"
     CDS             16378..18033
                     /locus_tag="ACHOQV_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9630043.1"
                     /translation="MSDTVKFKTIITTAGAEKLAASASDGGKKVTPAVMAVGDGGGTL
                     PDPDAKQTALINEVWRHDLNRVFIDNTSKNTVVAELIIPAETGGFWMRELGLYDDEGT
                     LIAVANMAESYKPRIAEGTGRSQICRMVIAVSNIETISLTVDSTVVTATKEYVDNLLE
                     EHEKSRNHPRASQAAPGLVQLSSQTDLDSEIMAATPKAIKIVMENANGRLAKNRNGGD
                     IPNKELFLINVGAPRVYAKDGYVGEAGQRWTTEQFVEWLNERGAFRPGLWIFRANISL
                     SRHSVLTDTGYGDICLGGCLIEVSGDINGGIIRITTGLESPNPADMYLRTQFTGVVLN
                     KGKSTIWYRTYTDKNKPTPQDIGALPASELLPAGVPLPWPSDTPPGGYAIMAGQTFDK
                     ARYPLLAKAYPDGRIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHGGSVSNTDLGTK
                     TTSSFNYGTKTTNSAGPFSATISLRTATASPEGSGDWTAYGPGSGANLIARVSEHTHS
                     VSIGEHIHTLALGQHGHGLTINPTGNAENTVKNIAFNYIVRLA"
     gene            18033..18758
                     /locus_tag="ACHOQV_001652"
     CDS             18033..18758
                     /locus_tag="ACHOQV_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP9630044.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENVWHLMEDHRGKTVYHEETGEKLVITGPGPLPSSVTTEPP
                     GTSADVYRDGQWVTDLSAARGIKNSEINDWRNRMENAGYIMEYNGRKWDYGKATQDRL
                     EPSVTAARAGKLPAEFFWTDAENNDVPMSADELIALSEAVGEAMFKKGLEIHIRQREM
                     KKELAGMDNAGEIMKFTPGWKIT"
     gene            complement(18801..19289)
                     /locus_tag="ACHOQV_001653"
     CDS             complement(18801..19289)
                     /locus_tag="ACHOQV_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9630045.1"
                     /translation="MMMIYGMFVFQLSTLPHQQLQQSRGWRHVRNERINRSAGWQYIG
                     AGEDKITLSGVLYPEITGGEVSLTVLNTQAYTGRPWPLIDGAGQIYGMYVITDIQTTR
                     EEFDRYGRAKKITFSVSFRRCDEDLRERLQASSVGDLLNNLKDAASAAGERVSSMASG
                     IL"
     gene            complement(19312..22107)
                     /locus_tag="ACHOQV_001654"
     CDS             complement(19312..22107)
                     /locus_tag="ACHOQV_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9630046.1"
                     /translation="MNTLDIRVAFSAADNLTRPATSARESAEALAASLRNTQNNLRDL
                     ERQSRSFSSARKHLTEVTDKIKRAQRELNGLQRLQQSGRPLTEAQQELINKLTARLER
                     LNEVRTQERNRLREAGQALRRHGVTLSGGDRAIEAAIRRTQQYNEELERQRLALARVS
                     RARDEYTRAMAVSEKLRGAGTAMMLAGLGGMYAGSRMVAPVMNEERYGAVIAAQTGEG
                     RAEASGYTRLVRELHHAGIAGDAARAAEAVGAVRSTLGALGVTGDTEISRITRRMMDM
                     QTVMGVDVPQGIQAAAIMMKNGLARSSDEALDLMVSAMQRMGPEMRGELPEIIHEYST
                     HFRNLGFSGAEAMSLLADMAGQGKFALDKTGDAVKEFSIRGSDMSKSSVEAYESIGLN
                     AAQMSSAIASGGVAARDAMQKTAAGLLRIRDPAERANAAIALFGTPVEDLAVDQIPAF
                     LGALARTSVVLGNTEGAAERMGNTLRDNLSGDVLKLTGSLAGLRADAVSTVTETLRGL
                     VQTATQWTARMREWVQKNPELVRGLLMVAGVVAGLSAAIGGLLVTVGMVLIPLVKLRL
                     GLSLIRALPAGISVVSGLFGGLGGVISSLGTAVGMLTGPVGLVVAALLGAGLLIYKYW
                     DQLRAFVLGFIDGARGALAPLLATVSRFAPLLDLVAGAVSKVFLWFKSLLSPVAATRD
                     TLDKCTAAGARFGSSLVSALTLVFSPMKALIDGLAWVLEKLGVLPGEAARVKQKLDAA
                     GKTPVVWEWDPEQKKMVQKAWNWSPKGAGGTQSVGVLRLPEMPAVLQPSANSGNGGVL
                     DRLKGIEGNTRDIVRETKKRTGPGDIIFRNLPPALVVRGQWTEPRLAGSTGVSGARIE
                     EHQNIKVASPPPLTVPSSGSERGLSSVGFSGEIHVHLHNVVTQDPRTLARMIGEAVKA
                     ELSGQSRNGLRSFRDVD"
     gene            complement(22104..22250)
                     /locus_tag="ACHOQV_001655"
     CDS             complement(22104..22250)
                     /locus_tag="ACHOQV_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP9630047.1"
                     /translation="MQFEHIEDVVADIAVVFNWSPADLFMMGPEDVIAWRKRASDRNG
                     SPEK"
     gene            complement(22259..22630)
                     /locus_tag="ACHOQV_001656"
     CDS             complement(22259..22630)
                     /locus_tag="ACHOQV_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003835883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFP9630048.1"
                     /translation="MNENAKLTKTAELVTDKDIGSNASPDVISLITPVVRDTETIKSV
                     KIGPLARQAGSLRGLSLVDLYGMKFDALLAYLSRVTEPRLKESELKAMDARDFSALAN
                     AAVNFYVGGVSTKPNGEETDG"
     gene            complement(22672..23184)
                     /locus_tag="ACHOQV_001657"
     CDS             complement(22672..23184)
                     /locus_tag="ACHOQV_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP9630049.1"
                     /translation="MAVPHKLRFMTLFIDGKNEIGQVVSVTLPKLTRKTESYRGGGML
                     GSVAVDLGLDDSALDASFVMGGAAADLVRKYAGRIDEVRLRFAGEYYTDGDSQLIEVE
                     MRGRISEIDMGEAKQGEDTQHTYNVKNTYYKLSSDDRELLEFDLLNFIYRQDGENIVP
                     DRIRSALGMG"
     gene            complement(23184..24371)
                     /locus_tag="ACHOQV_001658"
     CDS             complement(23184..24371)
                     /locus_tag="ACHOQV_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFP9630050.1"
                     /translation="MSETRFHGARVRESTDLVTAINDIDSSVIGIVAVADDADTGTFP
                     LDKPVLLTRVNDVLGKAGKAGTLYKALKAISDQVSTKVIVVRVAAEKKGDKQKTQDQL
                     VIGAVEADGRYTGMQALLVAEQDEAIGYRPRILAAPGLDTKAVTTALCTIAGKLRAFV
                     YAGCPGCNTATEAMTWRQDLAYRELMLIWPDFIAYNPQSGKNESFPSAAYACGLRARL
                     DHEQGWHKSLSNEPVKNVLGVSHPVYWSLQSEDSDANNLNNKEITTIIRRNGFRFWGN
                     RTTDTSAYIFEVYTRTAQTLADTIAEAQFEVVDDPLTPANVKDAISAIRAKLNELVTA
                     GKLIGAECWYDVIDNSTTDLRQGRVRIRYKYTPVPPLEDLTLHQTFTDEFFGPAFASL
                     GGA"
     gene            24533..25660
                     /locus_tag="ACHOQV_001659"
     CDS             24533..25660
                     /locus_tag="ACHOQV_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFP9630051.1"
                     /translation="MADRIIRIIKNYDDRTSTLVKLPDFSITLNNNELEAQELKNRVM
                     SLSVTDNRGFEADQISLTLDDTDGQLILPERGAVLSIAIGWKGESLTDKGTYIVDEVS
                     HDGPPDKLIIGASSADFREEFNVKREVSWHDVTLERVVSAIAHRYGLKAQISEMLMHI
                     EIDHADQTEESDMSFLTRMADMLGAIATVKNGNLLFIIPGGGVTASGKPLPSFTLTRS
                     DGDQHRFRIADRQAYTGVRAYWLDLNFGKKKQVSVKKRSLSQEKSHSREGDYMEGAEG
                     NVFVLRRTFPNEESAKRAAAAKWQQLQKGAAEFAITLAHGRADLYPEMHGTVSGFKTT
                     IDRQDWIIARVEHTIDSNGFTTQLELEAKIPDWIAESEQSG"
     gene            25704..25955
                     /locus_tag="ACHOQV_001660"
     CDS             25704..25955
                     /locus_tag="ACHOQV_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP9630052.1"
                     /translation="MFYCPHCGASARIRTSKAANDTGTIRYKYYQCNNIECGATFSTM
                     ESVHKMISVPTPSGERDEQIPHEHFPATHRGRLQLILEL"
     gene            26141..26281
                     /locus_tag="ACHOQV_001661"
     CDS             26141..26281
                     /locus_tag="ACHOQV_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9630053.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHNSPCEFRLNIMWSQFAAFLQC
                     KQ"
     gene            26610..26750
                     /locus_tag="ACHOQV_001662"
     CDS             26610..26750
                     /locus_tag="ACHOQV_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9630054.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSLWGAEFAAFLQC
                     KQ"
     gene            complement(27109..27195)
                     /locus_tag="ACHOQV_001663"
     tRNA            complement(27109..27195)
                     /locus_tag="ACHOQV_001663"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27159..27161),aa:Leu,seq:taa)
     gene            complement(27208..27281)
                     /locus_tag="ACHOQV_001664"
     tRNA            complement(27208..27281)
                     /locus_tag="ACHOQV_001664"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27247..27249),aa:Cys,seq:gca)
     gene            complement(27336..27411)
                     /locus_tag="ACHOQV_001665"
     tRNA            complement(27336..27411)
                     /locus_tag="ACHOQV_001665"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27376..27378),aa:Gly,seq:gcc)
     gene            complement(27563..28111)
                     /gene="pgsA"
                     /locus_tag="ACHOQV_001666"
     CDS             complement(27563..28111)
                     /gene="pgsA"
                     /locus_tag="ACHOQV_001666"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP9630055.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(28168..30000)
                     /gene="uvrC"
                     /locus_tag="ACHOQV_001667"
     CDS             complement(28168..30000)
                     /gene="uvrC"
                     /locus_tag="ACHOQV_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP9630056.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDESKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(29997..30653)
                     /gene="uvrY"
                     /locus_tag="ACHOQV_001668"
     CDS             complement(29997..30653)
                     /gene="uvrY"
                     /locus_tag="ACHOQV_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFP9630057.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         8150 a         8443 c         7757 g         6563 t
ORIGIN      
        1 accagaacac tggtcacaat taaccgcaaa gcggcaattg cccgctatgt gcattcagcc
       61 gctggcagac aatcacgcag gtaaacaaag accatgaaca taaccagaaa cagccaacta
      121 aaggagcgca ccatgaacga caccaggagg atcagcgaca gagagcttac gaaaatgatt
      181 gagtcggccc ggcaactgct ttcacgacaa aacccggtcg tggaagaaac gtggtgggag
      241 aatttacttg cagcactgac cgaactccgg gaacgccgga acaacgaagg agccgccaaa
      301 tgaagacccc aggaatgacc ggaaaggaaa tcattttacg ctacctggaa acgcacacac
      361 gtttcagcgc cccagaactg gtagaaaaac tgggcgtgaa aagaaccgtc attaatcagg
      421 ccgccgggaa aatggtacgg gaaggcctgc tggttgtgga cgggtacacg gacaagataa
      481 cgcattaccg gaagccaacg gaaaaggaac gacaggaact ggcgcaacgg gctgaacggc
      541 agcaggaaaa atcagtcatc gaagcctgca agcgcagcga aatcatgaag cgaattctgt
      601 ttatctacgg cgctggcaga gatctgccag tcatatcagc ccaataacta aaccaccaac
      661 ggagacaaat atgacaacgc agattgcaac acggggaaga ctggcggtat cactgccctg
      721 ccatggcgca gaacagccaa ccacaatgtg gctttacctt atcacattcg ggaagcagca
      781 cgaagaactg gcaaaacaca aaaaaaggcg atctggtaca ggtgcacggg aacttgcagg
      841 taaacaggta tcaggacaag ttcggacaac agaaagaggg ctggcaactg ctggcacaaa
      901 gcattatcag ctcaagaacc gcgcgatgac ttcacgcatg ttaccctcac tgttgagggt
      961 aactacgacg accgcacaga caacccagct tgataaagaa aaaaatgatg ttatgattaa
     1021 gtcataagta aggtgatacg aatcagttat gaaacacaat gatcatgggc tgaacaccct
     1081 cctggatatg catggatacc agcaccacat ggataatggt tactggtgga agattgaagc
     1141 atacagggtt agcccaaacc agttcaggcc acatggaata cgctacaacc tgacattgca
     1201 cgatccttat aactcccggg tatttggaat ggataacgcc cacggaataa agccaccaaa
     1261 gaaagggaaa tttaccgggc gcattaccgt ttatgaccac attcacagaa catcgtcaga
     1321 taaaggctat ccatatgaat tcacttctgc ggaacagctt ttactggatt tctttaacca
     1381 tgtagacaga gtgattgctg aacagcaaag ggggaagaaa tgaaagcccg tatcggcatt
     1441 atcccggaga aagtccttcg ccagagaatg ctggatatag ccaaagggaa actaaaacca
     1501 gaacctgacg aaccgcgagt ctggttttca tctctgaacg ctctgggtca agcgctgagc
     1561 aacgaaaata tcgcacttct gcgtatcatg gatgcggaaa aaccgcagac aatgacagaa
     1621 cttgccagtc tttcgggaag gcagttaagt aatttgtcag caacgttaaa aatgctgagt
     1681 cgatacggct ttgtcagcct ggaaaaacaa ggtaactgcg tacacccgaa agcgttgttc
     1741 acggattttg aaatcataat agacccgtca gtaggtgaaa ctcatcgggc agcgtgaata
     1801 ttgcgggggt aatccccgct catacttttt tagcctgcgt cacccgcctg aatacccacc
     1861 tgaaaaaatg cacaaaagtg cacgaaattg cacaattttt cttatgtatt ttttccccat
     1921 tcagcccggt aacggcgcgg gctggggcca gtttgcacgg tgcacgaaaa atgaggcaat
     1981 tgcagcgcgc aggtggcgtg ggacagggcg cacgaattga ggggtggaag gcaacttgct
     2041 ttatttgggg ccgtattgcg cgtgatacgc acacagtgaa gccagttttg aaaaaccaca
     2101 tcaaaacata gccagagaaa cagtccgtca gaatgcgtct ggttgcgtct ggagaggtcg
     2161 gggaagccag agaaaaagcg agcgtcgaac gtgatgaaaa agagggataa aaatcaccgg
     2221 aatcataaag ttaagagcat ttaaagtatc caggatccac gggtgaaaac acacgcacaa
     2281 tatgaggtga acgcagagtg caggcgggca tcaacagacc gaaggaggac ggtatgaacc
     2341 attttcgcga ctggtgtcag gcaaaccaga agagccagga cgcgagacgg gaggcgctct
     2401 gtattctccg ccgccagtcc aggctgcaaa gaaaagcgcc tccgcattcc gttaagagag
     2461 ctggtgagtc ggggcataaa ttttttatgc cccggcgaag cagcagacaa gcgaagcgcg
     2521 tcagttggta ttcgtactat ccggccatca cccgaacagg acgaacgcca cgacgtgtta
     2581 cacgagtacc ggagacagtc tggtgattct ggcccttaat ccggtaatct ggggctgacc
     2641 tgatataaaa ctgacacaag tctggtacaa actggatttg gattagattt aatttgaatt
     2701 aaatttggat ctgttttgta gataattttg atctgaacca tcgcgcattt tgtgaatgga
     2761 tgcagaccat tgccagcgag ccttaccggg cgctgccctc ctccagaagt gcatagggat
     2821 taaagcgcat cacctcgatc cccagccatt cattgacatg cttcatggcc tccatgacag
     2881 gttgcagctc gttgatgaaa aacaccctcg cagctttctc aacgtcaccg aacgccgcgt
     2941 tgccatcagg catggccccc atcagttgag gcggtacacg atgggccgcc aggatatcgt
     3001 cgcgggcact ccctttgatg ttcaggaatt catcctttgc ggttatctgc tggaatggca
     3061 gaatttgcac cccatccttg ccgccacccg gcgcatgaag aaacaggttt ttaaacgccc
     3121 cgcgaccacg cgaatccgtg agcgtttttt taatagcctc gacactttcc gcgttcgcct
     3181 gaggcgtacc aacatagata atacatccag catgcgagcc attatcgtag tagagcttgc
     3241 ggaacgtatc cgctgaattt gacaggctga cggacagcag cccaccaata tattccggca
     3301 tcccgtagat ctcctgatta atatcaggat taagcacgtg gcacacagcc cctttccgga
     3361 aactgaactc cgtaccgtcg tcgttgagat accagtaggt atccaggtca cttcccctgc
     3421 gtgtatattt tgccagtgcc gggcgaagca ggagtggttc ccccagcctg ttacgtcgta
     3481 cttcaaggta ggcattgcca aacacaaacc agtcaagcgc aaacgcggaa aacgcctgcc
     3541 gggaaagcag agaatgcgga ataaagcacc cattcagggc gttgcgcttg aagtacaaag
     3601 ccgactgatg ccaggacgca cgacgggatg cacgggcgat agcgttccag tccatggggg
     3661 tatcaaagta gcgcccgtta tctgcacatg acatgttatc caggagatcc atgcggctga
     3721 cttcataggg tccatcaaag gtgaatgcac tgagtgccgg tgttgatttc agtgcatcgg
     3781 gtagcgaatg ctgcccacgc tttccgatgt aatttttgcg tttactcatt gtgttcagaa
     3841 ctccattgca aagccacggt tatcctcccg ctctgcaccg attggctcat taatcacggc
     3901 aagcattgtt gcccaggcga ggtcaccatg gctgacacca cgggagcggt cggtgtcata
     3961 ggtgatatag ccgccgggtg tctgtatttt tcgcaccgat gcgaatgcct ggatcaggtc
     4021 acgttcactg cggtcatact cccagcgtcc ggcacgcacc acctgaagca ttttcatgac
     4081 cagtgcccgc ttggaggcga tattgaagtt gtacggcacg gccatcggga aaaacttttt
     4141 cacaatctgg aatacccctt caccgttccc gccggtcaca tcaataccga tatgtttcac
     4201 gttatatttt tgggtgtatt cctcaatcag ggccgcctgc tgttcaaact ccataccctg
     4261 taccctgacc gtctccacgg tacgaaaacg cccgcctgca accatcggtg gtacacatac
     4321 tgacaatgca ccacagtctc ccttaccgct actgccgttg gcgtcgtacc cgagccagac
     4381 ttcccgcacg cccatcggac gcgcagcaaa aggtttccag tcaggccatt cgtcgtatcc
     4441 atccacacca cagccgatca gcgcattcag actgaaagcc gcctcgccat cacgcacgaa
     4501 ctcgcacatg tacaggttac gaaattcgct ttcggtgttt tcatcccgga tatcgtcaat
     4561 gctcgtatat tcccacccgt tcgcgactgc atcctcaatg gtgacaatct gccgccatgt
     4621 tttatccggg aagagcacgc cccctttcag gttcgaatga gacacatcga attcagtgcg
     4681 ttttgagcgg ggacgctcac tgttccagcg atcccccgtc cagaactgat aagcttcatg
     4741 cgtttcaccc gatggcgttg aaaagtaggt acgggtaagc cctttcaggg tcgccattgc
     4801 gccagccacc tttcgcaact gcacaaaatt actgacccag aaaaattcat caaatttaag
     4861 gtgtccggta tacgactgag cggttgctgc cgatgtgcca aggaagtgaa gctctgcgcc
     4921 gttagacaaa acaatcttgt cccccccttt cagctcgaca tcaacttcca gcgccgcctg
     4981 ctgaataaag tgtttgaact gatacgcctg gcgacgggat gcggacaaaa atatctggtt
     5041 gcgctgatac ccggcagaga catcatcacg cagcgcatca aggagcgcct cgtgcgcaaa
     5101 ataccaggta gccccaatct gacgcgactt caggatcata cgattacggt ggtggcggtt
     5161 ttcataccat tcacgctgat ggcaggccag tgtctggagg gttctttcac gcagggcgat
     5221 gatttgctct tccgtaaagt gatttttcag tttgcgcttg cggggcttct taccggaaac
     5281 agcctgttca ccctgcccct cgttcagttt tttcagctga cgcgtcagca ggtcaatttc
     5341 cttaaaatcg ccccccgtct tatcggactt ggcggtaagc tggatcagtc gcgcatcgat
     5401 ggactgagtg acacgctgga tcgggggcgt ggcatcccag tcatcccgtt ttttccatga
     5461 ataaatcgtg ttctggttaa tccccatcag ttgagcgata tctgccggag tgtacccctg
     5521 ccagtaaagc tgtttcgccc gcatacggat aaatacatcc tggatcattg cgccccctgc
     5581 catttacacg gcaagattaa aacctcctta acacctcctg atacctgccg ccgttgtgcc
     5641 tggcgccata caacttcacc accttgagac actcccccgt acaccgccat catgagtaaa
     5701 acaactctgc tatggagaat ttctatggga agtcaaaagg cgccggagcg aaaaaaattt
     5761 cgcgttgccg tatccggttc aaccattgac ggacgcgaaa tttcggggga aatgctgcgc
     5821 cgcgctgcag aaaactacga cccgtccgtt tacggctcac gggttaatat cgagcacgct
     5881 ctttcgctgt ggccctccag tgagctctgc gcgatgggcg atctgatcgc actgtcaacc
     5941 gatgaaatca ccgaaggaaa actggccggg cgcacagcgt tatatgccga gattgaaccc
     6001 accgatcgca tgaaaaacat gctcgatgaa gggaagaaag tatattcaag cattgaactg
     6061 ttaccggacc ttgacgccgt tcaggggcct tacattgtag gcctggcaat gactgataca
     6121 ccggcaagcc tgggaactga gcgcctgaaa tttgccgcac agcagcgcgc cagcatcatg
     6181 cgcttcaata accgtacaag tgaaccctcc atgttcaccg cagccatgga agccgaactg
     6241 cttaccaccg cagagcagca gacagaggaa ggccgccagt ggttctctcg tgtcatgtct
     6301 ctcatcagca aagccagagg gacagacagc gagcagttca gccagatacg ggaagtggtg
     6361 gaaagtatcg cctccgatca tgccgacctg ctcaaccgct tcagcgctct tgagaagaaa
     6421 cacgccagcg gacaggaaac actgaaaaaa ctgcacgacg aactggcaga actgaagacg
     6481 caactgaaaa cccttgatgc tgatccgcag caacgtttta ttgccaccgg tggtaataac
     6541 cagattctgg ctgaattctg aaaggagaag attaacttat ggcccgttta tccctttccc
     6601 gtgaaggacg tgacagtctg gagcgctaca tgcttcagca ggcgcagctt gctggcatca
     6661 ccacagaacg gctgagcaaa acattcagcg ttgatccgtc aatccagcag aagatggaga
     6721 acgccatcaa ggaaagcgcc gaactgctgc agaaaattaa tgttatcggt gttgataacc
     6781 aggaaggtga aaaagtcctg attgacacca gtggccccat cgccagcacg aacgcaacga
     6841 gcgacggagt gaaacgccgt aatcctgcat cagttgccga cctggatgcg cgccgttacc
     6901 gttgcgaaca ggtgaactac gataccttca tcagttacgc acagattgat gcgtggagca
     6961 cccagaagaa ctttcagcaa ctactgagtg cacaaatcac ccgtcagatc gcccttgacc
     7021 gtatcatgat tggcttcaac ggcgagtcgc atgcactgat ttcagatcgc gcagctaacc
     7081 ctctcctgca ggacgtcaac cccggctggc tgaaacatat ccgcgacaag gcagacaccc
     7141 gcatagtgaa ggggatgacc ctgacccgtc gcgatgaaga aaacaaactg gtatcaaaag
     7201 gtgactatgg taatgccgat gccatggtgc acgacatccg ctcatctgtt ctggatgaat
     7261 ggcacaagga cgcgccggat ctggttgtac tgatgggacg cgacctgttc aatacactgc
     7321 gcctgccgat gattaatgcc atcagcacca ccaacccgaa cactgaactg gttgccggaa
     7381 agttgattgt ggcatcccgt gccatcggcg gtctgccgac gtatctcgca ccgtttttcc
     7441 cgaaagacgc catgctcatc acctcactga gcaacctgtc gatctacttc cagaaaggtt
     7501 cactgcgccg ttatatccgc gaagagccgg aatacaaccg gattgccact tatcagtcca
     7561 tgaatgactg ctacgtggtg gaagactacg gcaaatgtgc actgattgag gatatttcct
     7621 ttgccggtga agatccggat gagaagccgg acagcgagga atgacgacct gaagcaggcg
     7681 cacaggcata accggggcgc atacgcgccc gtaaatcagg agctgaagga tgctgacacc
     7741 ggcacaacgt tatgtgcaac aggcggtggc ccgtaaccgg gtaaccatga gtacacgaga
     7801 aacgcagcgg gaaagaaccg cacatgagca ggttcttcac cagttaaggc tggcacagca
     7861 acaactgaag ggtgttcaat caaacatcgc cagagcagaa ctcaaaagac acctgctgcc
     7921 tgactttgag ggatggatag agggaaccct agccggaaac agcggtcgcc aggacgaggt
     7981 tatcaccaca atgatggtat gggcgatcga ctgccgtgac tacccgctgg cgctgcgcat
     8041 cggggaatac gtgatccgtc atggtctggc cctcccggat aactttggtc gcgatgccgc
     8101 cactgtactc acagaggaaa ttgccgaaat cgtcctgaca caggccgcca caaacagcga
     8161 tgcagattta tcaggctata ccgtcatgct tgatacgcta cacgggctgg tacatgacca
     8221 ggatatgccg gatcaggttc gggccaaact gaacaaagcc cgcgcttttt cccgcagggc
     8281 atccactcag cctgatgaca tccatcagtc actgatgctg ttgcgggaag ccatgcgcct
     8341 gaatccggcc gcaggcgtga aacgggacat tgccactctg gaaagagccg tcaaaaagct
     8401 gtcttcgccc cctccggatc cggtcaaaaa aagcaccaaa accaccagga aaaccggcaa
     8461 cagtcaaaaa accagtgcga agaaaaagcc atccaccaga aagcccaaaa acacgcactg
     8521 atttaaacga actgacccac gtcagcaggc ggcgcaaccg gcgatctgta tgttttacac
     8581 atgcttttta ccggttgccc accgcctgat ttttacggag agagttttgt atgagcctga
     8641 tagcacggac accggcagag cacatcagtg aagacattcc tgatacagat gacggagaca
     8701 gctgtgtgac cgctgacgca ttctggcctg tcattgtatt acgggagctg agactggctg
     8761 ttcggcttcc tgggcgaaca accacggcgc gcctgatgca tgccgccaca gaggccgtcg
     8821 ctcacgttac cgccgaactg gagatgtgga aacaggaacg gatgaaagaa ggttacaccg
     8881 ccctggcaga tattccggca acgcatgtta acagtgaaag tgtgcatgtc caccgctacc
     8941 gcagggcggt gtatgccctt gcccgtgcat ttgttctcga acgtacacgg gatgtggaca
     9001 ccacagaaaa aggtgacaaa aaggctgact cactcgaccc acaggttgaa gctctctggc
     9061 gggatgcccg gtgggcaatt tcagatatac gcggcgtcac tcgcatttat gcggagcttg
     9121 tctgatgaaa atcaaagcgc tggaaggtga caccgttgat ctgctctgtt ttcgtcacta
     9181 cggcagtaca cagggcgtca cagagcaggt gcttgaagcc aatccgggat tatgtgagca
     9241 ggtttttctg gttgcaggcc aggaagtgga actgccggag caaatccgtg agaaaaaaca
     9301 ggaaacggta ttgctatgga gctgaatatt tttcagagaa tttacgacca catcaccttc
     9361 tcgatgtcac tcatggttac aggcatcggt gtgatgacgg tcagcgagaa gatagcagtt
     9421 gccggtcttc tgctgggcgc gctgtccggt cttcgcgcat ggatccacag ggcgcggctg
     9481 gaaaaagcgc aacgacgccg caatgaactg atagcacaaa tccttgcgca gtcagaatcg
     9541 cgccctctga acgcatcaga aaaacaggcg ctgaagcaac tggaggatca ggacgatgcc
     9601 ccgccacatt aagcgctatg ccgtagccac catcgtggcg ctgggcgtga ctctggcacc
     9661 gggcgccctg cgaacctctg aagaagcgca ggtaaaaatc gccacatggg aagactgtcg
     9721 ggcccggcct tactatgaca cggccggcat tcttaccact ggctgcggct caacaggaaa
     9781 cgtgaaaaat cggttataca gtgaaaatga ggtggctgag cggtgggtta acgacatgca
     9841 gcgcgcagag aactgtatca ataaaaactt ccacggtgcc gccatgcctc agtctgtgtt
     9901 tgaggctatg acggatgccg cctataacct gggctgtacc agtctgatgt ggtttaaaga
     9961 caggcacggg cagcgtcacc ggacaacgat atggaagcat gcacaggccc acagatggat
    10021 ccaggcatgt gcacgtctga ccgattttgt gaacagtggc ggcaaaagga tccccggact
    10081 ggttaaccgg cgcacagact ttaaggcatg gtgcctgaag gacacagggg ctacatcatg
    10141 aaaccggcag gcatcctctt tctgatggtc ttatgtatgg tcgcgattgc catcgcattc
    10201 cggcagacat ggcgactggg gcaggaaagc gcacgtaatg aggctctcca tgaaaccatc
    10261 agaagtaaca gcgccacact ggaatccatc atagctatca gccgcaacac tcacgcgctc
    10321 attgccggtc tgaaggtggc agaacaacat cgtaatcagg aaggagaagt cagacgtgaa
    10381 catatccgca atgccatccg cagcgatcgg tgcgctgaca ctcccgtgcc tgctgctatt
    10441 tctgacagcc tgcaaaaacg taccgtccgt ctctccccgg agctggtgcg aagcagtacc
    10501 ggagagcctc accacagtca caatgccacc accgctgacg gagccagtga catggggaaa
    10561 aatcgcagta tggagtgaca gcctgctgga tgcactggac agctgtaacg cagataaagc
    10621 agccatccga caactgaacg ctcaacagat ggcaacaaga gataacgagt aaccggagta
    10681 cagtatgaaa aaatacgaac tgatcacagc catgacgtgt gatgagctgg caaaagcact
    10741 ggatgaacgc ctggctgaag gctggcaacc gcacggagcc cccgtggcag catcagaacc
    10801 gggcgttccc ttctatctga tgcaggcgct gattaaatcc acggacactg caacgcaagg
    10861 cagcaacatc aacacaccca cggcagaacc ggagtattac tttgtgatcc ctgttgccgg
    10921 gcagtcaaac ggcatggcat acggcgaagg tctgccattg cccgaaacct gtgaccgtcc
    10981 ggacccgcga attaagcaac tggcgcgtcg cagtacggta actccgggcg gcgagtcctg
    11041 caggtataac gacatcatcc cggcagatca ctgcctgcat gatgtacagg acatgagcgg
    11101 gaaaaatcac ccgaaagcag acacatcgaa aggacagtac ggcactgtag gtcaggggct
    11161 gcacatcgcc aaaaaactgc tgccgttcct gccatctgat gcgggaattc tgcttgtccc
    11221 ttgctgccgt ggcggttccg cattcacggc aggcgctgac ggtacgtaca gtgacactac
    11281 tggcgcctca gggaattcag cccgctgggg tatggataag ccgctgtata aggacttaat
    11341 cagccggaca aaagcggcac tggcgaagaa cccgaaaaac cgcctgcttg ccgtggtgtg
    11401 gatgcagggc gagtttgata ttgatgcgaa gccgacggag cattccgcgc tgtttctggc
    11461 gatggtggaa aaattccgca cagacctggc tgaacaggcg gaacagtgta ccggtggcag
    11521 tgctgctggc gttccgtgga tttgtggcga taccacgtat ttctggaaac agaaaaacga
    11581 accggcatac caggcaatct atggcggcta caaaaataaa acagataaaa acattcattt
    11641 cgtcccatta atgacagatg aaaatggcgc gaatgtaccc accaacaatc cggcagaaga
    11701 cccggatatt gaatccattg gttattacgg ctcaacgtgg cgtaacagcg ctgccacctg
    11761 gacatctgcg gaccgggaaa gccacttcag ttcgtgggca cgcaggggga tcatttcaga
    11821 ccgccttgcc acagccattc tgacccatac tgtccgggct gctgcagtac agcctggtgc
    11881 atcatcttca gaacctgatg taccggaaac cgcagccacc accacgttac tgtcatatat
    11941 cgccagtgaa tcagaaggaa acctgaaagc acagggctgg tcggccagtg gcggtaaagc
    12001 agaaatcatc agcgataccg gagccacggg cggtaaggca atgaaactga ccaaacaaac
    12061 cggcaaagga tcctgggttc tggagcatga cgcaggcacg ggtgacagtc tgttacagaa
    12121 aggcggttta attcgttgtc gctttaaagc gcagggcgaa ctggcggcaa accggtacgt
    12181 catggcattg tactggccgg tatcctcact cccgcagaac gtcaccctgg cgggcaatgc
    12241 aggtaacaac ctgctggcgt cgttctacat ccagacagac gcaaaagaca tgaacgtgat
    12301 gtatcacaat gcaaaagccg cgaaggataa ccagaaactg ggaacatttg gcgcattcga
    12361 taatgaatgg cacacactgg cattccgctt cgcagggaat aacagccttc aggtaacacc
    12421 ggttattgat ggtaaggatg gggcaccatt cacactgaca cagtcacccg tcggaatgtt
    12481 tactgcagat aaactccacg taacagacgt cactaaaaat gccacttacc ccgtgctgat
    12541 tgacagcatt gccgtggaaa tcaataaccc ggtaaccgcg taaaaaaaaa tcccgccgct
    12601 cattacagct ggcgggaggt agtaataccc aacccattaa aaatttgtaa ggagtaccca
    12661 caaaggagta aatgtcagtt gtcttactgt tttacccgat aacgatttat acggccaata
    12721 tcaaagacag ggttatatgg atcccgttta cacaatactg ctctgctttt gcaccatcac
    12781 ctgcgtgctg ttccttgcca gtggtggcgg agaaatcctt tatcaatggt gcgcaaaaaa
    12841 acttcatgaa tggctgaaca agtctgaacg tgacacgcca gaccagtgat ctttaccgga
    12901 gaggtgacac atgctcaaga tgaacgcatt gcgcgatacc ctggctgaca catgccgctg
    12961 gtgtaaagcc aatccggata aattcactgt ctgcgtggaa tccggcagca ttgaaacgac
    13021 cggtgaatca ccctcgtttc tgtaccgtta caacctggtg atctttgcca tggacttcac
    13081 tcaggatatc gacgaactgt tcctccctct gctgcactgg ctttaccaca accagccgga
    13141 tttactgctg aatccagaaa aaaacagcag catcaaattt tcagcatccc cgaataacga
    13201 cgacagtgtt gacgtggtta ttgagctgcc ggtatgggaa cgcgtgaggg tgacacgcac
    13261 acctgacagg caagtccgcg cagaacatct gcaggagccg cggccccgca tcccctcagc
    13321 acggggagcg tggagcaatg tatacgaaga cgtaacatgg agtatacccc gtgatgaatg
    13381 agcacaaact tgatgctgtt tttcgtgaca ttctggccgg tatgtccgcc gcaggtctgt
    13441 cccgcacggc cagacaggtg gggcaggcag tacgccgcag ccagcagcag agaatgcgtg
    13501 cacaaaaagc cccggatgac accccctggg caccacggaa acgtcagatt cagcgggtac
    13561 aacagggtat taagtttctc tggcaacctc cggcagcggg cagccagccc atggtcaggg
    13621 aactgaaaaa ctggaagcat ggtacaggtc gccacggccc acacataacc ggttatgaca
    13681 ttaaccgcgg gggactcaga acattctacc gggctgacat tgaagaatac ctggaaatca
    13741 gaacacgccg tgtcagccag ggaaacaccg tcagggcacg gatgtttgaa cgcctgcgac
    13801 ggtacgtaaa aatacttcag gccgacaatg agagcatccg ggtgggtttt aacggtgatc
    13861 aggcccgtat tgcgcgtgta caccatttcg ggttacgtga caacgttgtc cctggtgtaa
    13921 caacacaata tccggcccgt gaactgctgg ggttgtcagt aaaagataaa gcaatgatcc
    13981 gggaaaccat catcggaagt ctgaaaggag ggatgaagtg agcgcggaaa ttatgcgcct
    14041 gctggaaaat atgatccggg tgggtgtgat ttcagacgtt gatttaaaaa actggcgtgt
    14101 aagagtgacc agcggcaggc tgacaacgga ctggctgcgc tggaactgca cccgtgccgg
    14161 tgtatttaaa atctggcttc ctccttctcg cggtgaacag gttttactgc tctgccctgg
    14221 cggtaaccct gaaatggcaa tggttgtcgg gagcctgtac agcacagaca accctgcccc
    14281 cggaacacag caggatgaag tcatcatcac tgccagagat ggtgcaagac tccgttataa
    14341 cgcaaaggaa ggtgcccttg acgcaacagg gataaaaacg gccagagtga cggcagaaac
    14401 ccgaatcact ctggaaaccc cgctcgttga gtgcacacag cacctgaaaa cgcgcacatt
    14461 tgaactgaca gatggcggca cactgaaagg tgacgtaacc cactcaggtg ggtcactgac
    14521 atcaaacggc gtggtcacac acacccacac acactcaggc gtccagtccg gtgggagcca
    14581 gacaggaggc ccacagtgac agccacctac acgggtatga acccttacca tccgggtgtt
    14641 gtgcacgata cagagcatct gcactgttct gtacgcgata ttctgctcac acctcaggga
    14701 agccgcatta tgcgcaggga ttacggcagc ctggtacccg atcttctgga tgccccccag
    14761 aatgatgtaa cccgccttca gtgcatgagt gccgcagtga tcgccctgac ccgccatgaa
    14821 ccacgcctgg cactgaatac catcgacgtt cgctggctgg atgaaggtgg ggctgagctt
    14881 ttactgaccg ggataatcac cgcaaccatg cagacggtca gactgagcat acgcacagga
    14941 gactaaccga tgccaaccgt tgatttgtca cagttgccga aaccggcaat catcaacaca
    15001 ccggattttg aagttattct caatgagata aaaacgatga tgatcggctg ctttccggaa
    15061 caacagcaac ctgctgtaac cgctgctctc gcactggaat ctgaaccact gaccattatc
    15121 gccgaggtaa tggctttccg ggagatgcta ctgcgccagc gtatcaatga aggtgttgca
    15181 gcctgcatgt taagccatgc ctccggtaca gatcttgatc acctggcagc caacctgaat
    15241 acgcagcgcc tggtgatcac tccggcaaca gatactgccc cggctgtcat ggaaaacgac
    15301 accgcactgc gcttacgtgc gcagtcagcc tatgaaggcc ttagcgtggc aggaccatcg
    15361 gcctcttatg aatactttgc ccgaagtgcc agtggtcagg ttgctgatgc cagggcagtc
    15421 agcccatccc ctgcagtagt ggtggtgtca attctctcaa cagaaggcga cggcactgca
    15481 tcgcaggaac tgattcagac cgtcagtaac gccctcaacg atgagcgaat caggcctgtc
    15541 gcagaccggc tgaccgtcca gagtgcaaaa atcgtaaaat accggatcaa tgcccggctc
    15601 tttctgtatc cggggccgga gtccgaacct gtaattctgg ctgccagaca gtcccttgac
    15661 gaatggctga ccgaacaggc ccacataggc cgtgatgtgg cccgctctgc gatcatgtca
    15721 gcattgcatg tacagggcgt ccagcgggtg gagctggagg aaccagcaag caatatcgcg
    15781 gttgacgact cgcagttcgc acgctgcgaa tccattatcg ttgatatggg aggtaccaat
    15841 gagtgattcc ctgcttcctc cttcagccag tgactttctg aaatataccg gatatgccac
    15901 tgaccgaatc acccgtatcc cggttgattt gcgtactctc tgggatccgg aagcctgtcc
    15961 ggtggagctt ctcccctatc tggcctgggc gctgtctgtc gagcgctggg ataaaaactg
    16021 gccggaggaa acaaagcgaa aagttatcag ggaatcctgg ctcatccacc gtcacaaagg
    16081 taccttcagc tcactcaggc gcatcgttga acctctgggt tacatcatca gcatgagcga
    16141 atggtgggaa accggcgatc cacccggcac ttttcgcctg aatgtgggcg tgtttgaagg
    16201 ggggctgact gaagagatgt attttgaaat agagcgacta atcgccgatg cccgccccgt
    16261 cagccgtcat ctggtaggcc tgaatatcgt gcaggacatt tccggcaagg tatacacagg
    16321 cggcatggtg tatgacggcg atattattac cgtttacccg atacaggaga gcgacgcatg
    16381 agtgatacgg taaagtttaa aaccattatc acgactgccg gtgctgaaaa gctggcagcc
    16441 tcagcctctg acggcggcaa aaaagtcacg ccagccgtta tggctgtagg tgatggcggc
    16501 ggaacactgc cagatccgga tgcgaaacaa acagcgctca tcaacgaagt ctggcgacac
    16561 gatctgaaca gggtatttat tgacaatacc agcaaaaata ccgtagtggc agaactcatc
    16621 attccggcag aaacgggcgg tttctggatg cgtgagctgg gactttatga cgatgaagga
    16681 acactcatcg ccgttgccaa tatggcagaa agctacaaac ccagaatagc tgaaggcaca
    16741 ggacgttcac aaatttgccg catggttatc gcggtcagta acattgaaac aatcagtctg
    16801 actgttgatt caacggtcgt taccgcaacg aaagaatacg ttgataatct gcttgaagag
    16861 catgaaaaat cgcgtaatca cccgcgcgcc agtcaggccg caccgggatt agtacaactg
    16921 agcagtcaga cagatcttga ctcagaaata atggctgcca cgccaaaagc gataaaaatc
    16981 gtcatggaaa acgccaatgg tcggcttgcg aaaaaccgga atggcggaga catccccaac
    17041 aaggagctgt tcctgatcaa cgtgggcgca ccacgcgttt atgcaaaaga cgggtacgtg
    17101 ggtgaagcag gacaacgctg gacaacagaa caattcgtgg aatggctgaa tgagcgtggc
    17161 gccttcagac caggcctctg gatattcaga gccaatattt cactttcccg gcacagcgtg
    17221 ctcactgata cgggctatgg tgatatctgc cttggaggct gccttatcga agtatcaggc
    17281 gacatcaacg gggggatcat caggatcacg acaggtctcg aaagcccaaa ccctgccgac
    17341 atgtacctcc gcacacagtt caccggggtt gttttaaata aagggaaaag caccatctgg
    17401 taccgcactt acacggacaa aaacaaaccc acaccacagg acatcggcgc actgcctgca
    17461 agtgagttgt tacccgcagg tgttcccctg ccgtggccgt cagatacccc gcctggcggt
    17521 tacgccatca tggcgggaca gacattcgat aaagcacgat atcccctgct ggcaaaagcc
    17581 tatccggacg gtcgtatccc ggatatgcgt ggctggacaa tcaagggtaa acctgccagc
    17641 ggtcgtgcgg tattgtcaca ggaacaggac ggcatcaaat cgcacaccca cggagggagc
    17701 gtctcaaata ctgaccttgg cacaaaaacg acctcatcct tcaattacgg cacaaaaaca
    17761 acaaacagcg cggggccgtt cagcgcaaca atatcattaa gaaccgccac cgcgtcaccg
    17821 gaaggcagcg gtgactggac cgcatacggg ccgggctccg gggcgaacct gatcgccaga
    17881 gtttcggaac acacacattc ggtaagcatt ggcgaacaca tccacacgct cgcactgggt
    17941 caacatggtc acggcctgac cattaacccc accggtaatg cggaaaacac cgtcaaaaac
    18001 atcgcattta actacatcgt gaggcttgca taatgacctt taaaatgagc gaacacccac
    18061 agacaattaa aatctacaac ctgtcagccg ggacaaatga atttatcggc gaaggcgatg
    18121 cctggatccc tccgcatacc ggactgcctg cgcactcaac ggacattgcc ccgcctgaca
    18181 ttcctgaagg acacgtggct atctttgatg aggacgaaaa cgtctggcac ctcatggaag
    18241 atcaccgggg taaaacggtt taccacgaag agaccggaga aaagctggtt ataaccgggc
    18301 cgggtcccct gccatcatcc gtgacgaccg agccacctgg tacttcagca gatgtatacc
    18361 gtgacgggca atgggtgaca gatttatcag cggcgcgcgg gatcaaaaac agcgaaatta
    18421 acgactggcg taacagaatg gaaaacgctg gttatattat ggaatacaat ggccgcaaat
    18481 gggattacgg caaagccacg caggatcgcc ttgagccatc tgtaacagcc gccagagccg
    18541 gaaagttacc ggcagagttc ttctggacag atgcggaaaa taacgatgta ccaatgagtg
    18601 cagacgaact gatagcgctc agcgaggccg tcggagaggc aatgtttaaa aaaggacttg
    18661 aaattcacat ccggcagcgg gaaatgaaaa aagaactggc cggaatggat aacgcggggg
    18721 aaataatgaa attcactccg ggctggaaaa taacatgaca gaatgaggcg gcagacttca
    18781 tgatgctctg ccgccggata tcacagaatg ccagaagcca ttgaggaaac ccgttctccc
    18841 gccgcactgg cagcgtcttt caggttattc agcaaatccc ccacagatga tgcctgaaga
    18901 cgctcccgca aatcctcgtc acaacggcgg aaactgacag aaaacgtaat ttttttcgcc
    18961 cgtccatagc gatcaaactc ttcacgggtc gtctgaatat ccgtaatcac atacataccg
    19021 taaatctgtc cggctccgtc aatcaacggc caggggcgcc ccgtatatgc ctgagtgttc
    19081 agcaccgtaa gggaaacctc cccgcccgtg atttcgggat acagcacacc ggaaagcgtg
    19141 attttatctt ctccggcccc gatatactgc cagccagcag aacggttaat acgctcattt
    19201 ctgacatggc gccagccccg gctctgctgt aactgctgat gtggcagtgt actgagctga
    19261 aaaacaaaca taccgtaaat catcatcatc tctcataccc tccatcatcc attaatccac
    19321 atccctgaaa ctacgcagcc cgttacggga ctgaccggac aattccgctt ttaccgcttc
    19381 gccaatcatc cgggcaagag ttcgcggatc ctgggtaacg acattatgca gatgaacatg
    19441 gatctcccca ctgaacccca cagaagacag tccgcgttct gagccagatg aaggcacagt
    19501 gagcggtggt ggactggcaa ccttaatgtt ctggtgctct tcaatacggg ctcctgaaac
    19561 accagtactg cccgccagtc ggggttctgt ccattgtccg cgaacaacca gcgccggagg
    19621 aagattacga aaaataatat caccaggtcc cgtgcgcttt ttcgtttccc tcacgatgtc
    19681 ccgtgtatta ccttcgatac ctttcagcct gtcaagaacg ccaccattcc cgctgtttgc
    19741 ggagggctgc aacacggctg gcatttcagg taagcgtagg acaccgacac tctgtgttcc
    19801 tcctgctcct ttcggcgacc agttccacgc cttctgaacc atctttttct gctcagggtc
    19861 ccattcccag accacaggtg ttttaccagc cgcatccagt ttctgtttca cgcgcgctgc
    19921 ctctccggga agcaccccaa gcttctccag cacccaggca aggccatcta tcagcgcctt
    19981 catcggtgaa aacacaagcg tcagagcact gaccagagaa ctgccaaaac gtgctccggc
    20041 agctgtacac ttatccagcg tgtcacgcgt tgctgcgaca ggactcaaca gcgatttaaa
    20101 ccacaggaat accttggata cagcaccggc aaccagatcc agcaacggtg caaaacggga
    20161 caccgtggca agcaaaggtg caagcgcccc tctcgcacca tcgataaacc ccagaacaaa
    20221 cgccctgagc tgatcccagt atttatagat cagaagcccg gcacccagca gcgccgccac
    20281 aaccagaccg acaggtcctg taagcattcc caccgcagta cccagggaag aaattacgcc
    20341 ccccagacca ccaaagagac cagaaacaac agaaataccc gcaggcagag ctcttatcag
    20401 actcagacca agacgcagtt ttaccagggg gattaacacc ataccgacgg taacaaggag
    20461 tccgccgatg gcagcagaca gcccggcaac aacaccggcg accataagca gtcccctgac
    20521 cagttcagga tttttctgta cccactcccg cattctggct gtccactgcg tcgccgtctg
    20581 taccagcccg cgcaatgttt ctgttaccgt actgacagca tcagcccgta acccggcaag
    20641 cgaacccgtc agcttcagaa cgtcaccgga gagattatca cgcagcgtat tacccattcg
    20701 ctcagctgcg ccttctgtgt ttccaagtac gacagaagta cgggcaagtg cccccagaaa
    20761 tgcagggatc tgatcaactg ccaggtcctc aacaggcgta ccaaacaacg caatagccgc
    20821 attagcccgt tcagccggat cgcgaatgcg cagtaatccg gctgctgttt tctgcatggc
    20881 atcacgggcg gcgaccccgc cactggcaat agccgaagac atctgcgccg cattaagccc
    20941 tatgctttca taagcctcga cgcttgactt cgacatatca gaaccacgaa tactgaactc
    21001 cttcacagca tccccggttt tatccagtgc aaatttcccc tgaccagcca tatcagccag
    21061 cagactcatc gcctcagcac cgctgaaacc cagatttctg aaatgcgttg aatactcatg
    21121 aataatttca ggaagttcac cacgcatctc cggccccatg cgttgcatgg cagagaccat
    21181 cagatcgaga gcctcatcac tgcttcgcgc cagcccgttt ttcatcatga tggcagcagc
    21241 ctgtatcccc tgaggaacat ccacgcccat cactgtttgc atatccatca tgcgacgggt
    21301 gatacgggag atctctgtat cccccgtaac tccaagcgcc cccagcgtac tacgaacagc
    21361 tcccactgct tcagccgccc gggcagcgtc accagcaata ccggcatgat gtagttcacg
    21421 aacaagacgt gtatacccgg atgcctcagc acgcccctct ccggtctggg cagcaatcac
    21481 tgctccatag cgctcttcat tcatcaccgg tgcaaccatg cggctgcccg catacatccc
    21541 gccaagaccg gcaagcatca ttgctgtacc agcgccacgc agtttttcag ataccgccat
    21601 cgcacgggta tactcatccc ttgcccggct gacccgcgca agcgccagac gctgccgctc
    21661 gagctcttcg ttatactgtt gagttcgcct gattgccgcc tcaatggccc tgtccccacc
    21721 agacaacgtg acaccatgtc gcctgagcgc ttgcccggcc tcccgtagtc tgtttctctc
    21781 ctgcgttctg acttcgttca gccgttcaag tcgtgccgtc agcttgttaa tcaattcctg
    21841 ctgcgcttca gtgagcggac gaccgctctg ctgaagacgc tgaagcccgt tcagttcccg
    21901 ctgggcgcgc ttaattttgt ctgttacttc ggtgagatgt ttgcgggcag atgaaaagga
    21961 gcgggactgt ctttcaagat cccggagatt attttgcgta ttgcggagag aggcggccag
    22021 cgcttctgca ctttcacggg cactagtggc tggccgggtc aggttatcgg ctgcactgaa
    22081 tgcaacacga atatcaagtg tattcatttt tctggagatc cgtttctgtc agacgcccgc
    22141 ttacgccagg cgatgacatc ctccggcccc atcataaaca ggtcagcggg cgaccagtta
    22201 aaaacaactg caatatcagc gacaacatcc tcgatatgtt caaactgcag gatggtgatc
    22261 agccgtccgt ctcctccccg ttcggttttg tcgacacacc accgacataa aaatttacgg
    22321 cagcattcgc aagcgcagaa aaatcccttg catccattgc cttcagttca ctctctttca
    22381 ggcggggctc tgtcacacgg gagagatatg ccagcagcgc atcaaatttc atgccataca
    22441 aatccaccag agacagcccc cgcagcgaac cagcctggcg ggcaagtggc ccaatcttca
    22501 ctgatttaat ggtttcagta tcacgcacaa ccggagtaat caggctgata acatccggag
    22561 acgcattact tccgatatct ttgtcagtga ccagttctgc cgttttcgtc agttttgcat
    22621 tttcattcac gttttattct cctggcggta tggctaccgc cgtctgttct ttcaacccat
    22681 ccccagcgcg gaacggatgc ggtcgggaac aatattttcc ccgtcctgtc ggtagatgaa
    22741 gttcagcaaa tcgaactcca gcagttcacg atcatctgaa ctcagtttgt aatacgtgtt
    22801 tttcacattg taggtatgct gggtatcttc tccctgtttt gcttccccca tatcgatttc
    22861 actgatgcgt ccgcgcattt caacctcaat caactgactg tcgccatcgg tgtaatactc
    22921 tccggcaaaa cgaagacgga cctcatcaat acgtccggca tacttacgca ccaggtcagc
    22981 agctgcacca cccataacaa aactcgcatc cagtgcgctg tcatccagtc cgagatcaac
    23041 agccacactt ccaagcatac ctcccccacg gtagctctcc gttttgcgcg tcagtttggg
    23101 taacgtgaca gacaccacct gaccaatttc gtttttaccg tctatgaaaa gcgtcataaa
    23161 acgcagctta tgcggaaccg ccattacgca cctcccagag aagcaaatgc aggaccaaag
    23221 aattcatccg taaaggtctg atgcagagtc agatcttcaa gtggtggcac aggcgtgtat
    23281 ttataacgaa tacgcaccct tccctgacgc aaatccgttg tgctgttatc aatcacgtca
    23341 taccagcatt ccgccccaat cagttttccg gcagttacca gttcattaag ttttgcccga
    23401 atggcgctga tggcgtcttt cacatttgca ggcgtgagcg gatcatcaac aacctcaaac
    23461 tgcgcttcgg cgatggtatc cgccagagtc tgtgcggttc gtgtatagac ttcaaaaatg
    23521 taggcgctgg tatccgtggt acggtttccc cagaagcgaa aaccattacg tctgataatt
    23581 gtggtgattt ctttgttatt gaggttattg gcatcactgt cttctgactg gagagaccag
    23641 tagaccggat gagaaacacc cagcacattt tttaccggtt cattagacag cgacttgtgc
    23701 cagccctgtt catgatcaag acgggcccga agaccgcagg cataagcagc agacgggaag
    23761 ctttcattct ttcctgactg tgggttatag gcgataaaat ccggccagat cagcatcagt
    23821 tcccgataag caagatcctg acgccaggtc atggcttctg tggcagtatt gcagccggga
    23881 caccctgcat aaacaaacgc ccgcaattta ccggcaatcg tgcacaacgc agtggtaacg
    23941 gcttttgtgt caagcccagg cgccgccaga atacgcggac gatacccgat agcctcatcc
    24001 tgctctgcta ccagaagcgc ctgcataccg gtatagcgac catccgcttc aaccgcacca
    24061 ataacaagct ggtcctgagt cttctgtttg tcacctttct tttctgccgc cacacgaacg
    24121 acaatcacct ttgtgctgac ctgatcggat atcgccttca gggctttata cagcgtacct
    24181 gccttgcctg ccttgcccag cacatcattc acacgggtca gcagaacagg cttatccaga
    24241 ggaaacgtgc cggtatcggc atcatctgcc acagcaacaa taccgatcac acttgagtcg
    24301 atatcgttaa ttgccgtgac gagatcggtg ctttcccgga cacgcgcccc gtgaaaacgt
    24361 gtttcgctca tacatttcac catcatcttc agaatgaatt cacgatgatg attacgcaga
    24421 tacacgggga gttcacgata tacaggttgt gaatcatcac atacaacctg ccggggttcc
    24481 ggccagacgg cggttatgag attttcgcca cagataacgg gagaacacaa caatggctga
    24541 tcggatcatt cgcatcataa aaaactatga tgacaggacg agtaccctgg taaagcttcc
    24601 tgatttcagc atcacgctga acaacaacga actggaagca caggaattaa aaaacagggt
    24661 aatgtcgctg tctgtaactg acaacagggg atttgaagct gatcagatat ccctgacact
    24721 ggatgataca gacggacaat taatcctgcc ggaacgggga gccgtacttt ccatcgccat
    24781 tggctggaaa ggcgaaagcc tgacagataa agggacatat attgtggatg aagtttcgca
    24841 tgacgggcca ccggacaagc ttatcatcgg agccagtagt gctgacttcc gggaagaatt
    24901 caatgtcaaa cgggaagtat cctggcatga cgtaacgctt gagcgtgtgg tttccgccat
    24961 tgcacaccga tacggtctga aagcacaaat cagcgaaatg ctgatgcata tcgaaatcga
    25021 ccatgcagac cagacagaag agagtgacat gtcattcctt acccgcatgg ccgatatgct
    25081 tggcgccatc gcaacggtca aaaacggcaa cctgctgttc atcatacctg gtggcggcgt
    25141 gacagccagt ggcaagccac tcccctcttt caccttaacc cgcagcgacg gtgatcaaca
    25201 tcgcttccgt atcgctgacc ggcaggcata cacaggggta cgggcatact ggctggatct
    25261 gaatttcggg aagaaaaagc aggtcagtgt aaaaaaacgc agtctgtcac aggaaaaaag
    25321 ccacagccgt gaaggtgatt acatggaagg cgcagaaggc aatgtgtttg tgttgcgaag
    25381 aacctttccg aatgaagaaa gcgcaaaacg tgcagcagct gcaaagtggc aacaattaca
    25441 gaaaggcgcg gctgaatttg ccatcacact ggctcacgga cgcgcagatc tctaccctga
    25501 aatgcacggc accgtatcag gattcaaaac cacgatcgac cggcaggact ggatcattgc
    25561 cagggtggaa cacactatcg acagcaacgg tttcaccaca caactggagc ttgaggctaa
    25621 aatacctgac tggatagcag aatcagaaca gagtggataa aataaaccac gagttcaacc
    25681 ccgtaatgac tgggggtatc gtcatgtttt attgtccaca ctgcggcgca tcggcccgta
    25741 tcagaaccag caaggcagcc aatgataccg gcaccatccg gtataaatac taccagtgca
    25801 acaacatcga atgtggtgcg acattctcca caatggagag cgtacacaaa atgatatccg
    25861 tacccacccc ctccggcgaa cgggatgaac agatcccgca cgagcatttc ccggcaacac
    25921 acagaggtcg ccttcagcta atcctcgagc tctgacagtt gcgaccacaa aaggaaatca
    25981 gtacattaca cgcgggtgcc tttcggctga cggctggagg gtgaacctga aggccggatg
    26041 tggaaaggcc ccgagtcaac ttaaacgtta acccgaggcc ctgaccatcc tacctagcaa
    26101 gtggaaggtt agcgcctccc tgtaaaagga gtcaagcgct atgccgcaaa aaacgatcat
    26161 agtgagcatt ttatgcataa ctattttgat gattgtctgg atgatacaca actcaccctg
    26221 tgagttcagg cttaacatca tgtggtcaca atttgcggcg ttcttgcagt gtaaacagta
    26281 agaaaccgca gcgggggtga aagcccccgc tatccggttg ccgggttcag tcgatatggc
    26341 ccccttttct ccattggatt acagacgtat ttgcccgcaa tatgcgggct ttttttgccg
    26401 ccccctcaac agcttcaaca cagagcattg cgcacacaaa gggaaatcag tagattacac
    26461 gcgggtgcct ttcggctgac ggctggaagg gatacctgaa ggtcggatgt ggaaaagccc
    26521 cggaaaaaca cttttgttta accgaggccc taaccgcatt accttgacaa gtgaaaggtt
    26581 agcgcctctc cactaaggag gcaagcgcca tgtcgcaaaa atcgctgaca gccatcacgt
    26641 tctgcgtgac ggcaatcctc atcatctgga tgctgcacgg ttcactgtgc gaaatacgga
    26701 tgagcttatg gggagcggag tttgcggcgt tcttacagtg taagcagtag gaaaccgcga
    26761 cgggagagca atcccccgtc aatcggttgt taaggttaag gctgaaaagg caccctatct
    26821 gttacttgaa atgaaaagca cagcccgcag cgtaaaaact gcgggctttg tttttcagta
    26881 ctatcgaaaa cagcccacac ctacaaacag tgaatagtac agagagcaat aagaaaccag
    26941 caataacagg aaagacgatt gccactgagt caactttact cccgcgccta catgtcgcca
    27001 taccatcgcc actttatcgc cacaagccaa aacacaatca caaaaaaacc accctaaggt
    27061 ggtttcacga cactgcttat tgctttgatt ttattcttat ctttcccatg gtacccggag
    27121 cgggacttga acccgcacag cgcgaacgcc gagggatttt aaatcccttg tgtctaccga
    27181 ttccaccatc cgggctcggg aagaaagtgg aggcgcgttc cggagtcgaa ccggactaga
    27241 cggatttgca atccgctaca taaccgcttt gttaacgcgc caaattcttc aggcctttca
    27301 gccagacatc cgcttgacgc cgatgtcttt taaactggag cgggaaacga gactcgaact
    27361 cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg cattcatcaa
    27421 gcaatcagtt aatcacttga ttttattatc gtctggcaat cagtgccgcc gttcgatgcg
    27481 ttgcattcta cttacctggc gcgatgagtc aacgatattt ttcaccactt ttgatcgttt
    27541 gctgaaaatt gcgccgaaac gatcactgat caagcaaatc tgcacgcgca gcgctcaaat
    27601 attgcaacat tgaccacaga gtcagtaccg cagccacaaa gaaaagtgca ataccggcgt
    27661 actcaaccca aatgttcgga cgccacagca gccaggccag cgccaccatc tgggcagtgg
    27721 ttttcacttt cccaatccag gagacagcca cgctactgcg tttacccaac tccgccatcc
    27781 attcgcgtaa cgcagaaata ataatttcac gggcgatcat cgttgctgcc ggtaaggtca
    27841 cccaccagct gtgataatgc tcggttacca gcaccatggc gatagccacg agaactttat
    27901 ctgctacagg gtcaaggaaa gcaccaaacc gggtactctg gttccagcgg cgtgccagaa
    27961 aaccatcgaa ccagtcagtc accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg
    28021 accaggtgac aggcagataa aagaccaata caaagaatgg gataaggatg acacggaaca
    28081 gtgtaagcaa cgtagggata ttaaattgca taatgacggg taactatctg ttgtcagtaa
    28141 gattacccct atgttgctac agagacatca atgtttcaac gaccagaaga tcttttctgc
    28201 cagaccttgc gaaatacccg gcacttttgc aatttcctcg acgctggcgt tacgtaaacc
    28261 ttgcaaaccg cccatatatt tcaacaacat ttgccgacgt tttggcccga cgccttcaat
    28321 ggtttccagg gaactggtat ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc
    28381 gtgatcgtgt gattcatcgc gaatatgctg gataacatgc agtgcgggag aatctggcgg
    28441 caaactgaat ccctcacctt ccggctcaaa gaacagcgtt tccagcccag ccttacgatc
    28501 tgctcctttg gcaacgccta gtagcagcgg atgattttta tcccatgaga catccagttc
    28561 ggcgaaaaca tttttcgcct gcgcaagttg gcctttgccg ccgtcgataa ggatcacatc
    28621 cgggatctta ctctcgtcga tggctttacc ataacgccga cgcagcacct gattcatcgc
    28681 cgcataatca tcgcccggcg tgatgccagt aatgttatag cgtcgatact ccgcacgcag
    28741 cgggccgtta gcatcaaaca ccacacagga agcgacggtt tgttccccca tggtatggct
    28801 gatgtcaaag cattccatcc gcttcacttc cggcaatttc aacacgctgg caagcgcggt
    28861 cagtcgctgg tgaacggtag attgctgcga aagtttgctg gttaaggccg tcgccgcatt
    28921 ggtgcgcgcg agtttcagat aacgcgcccg atcgccgcga ggtttggttt gaacattaat
    28981 cttgcgtccc gccagttctg aaagggaatc ggcgagcagc gttttatcgc taagattaaa
    29041 atcgagcagg atctcacccg gtaaggtgcg catctggctg ccttgtaaat agaactgacc
    29101 tacgaaggtt tccaccacct cgctcagttc cgtaccgcca ggcactttcg ggaaatagct
    29161 gcggctgccg agcactttac cctgacgaat gaacaatacg tggacacaag ccatgcccgc
    29221 atcgaacgcc acaccaataa cgtcgaggtc gtcgccggta ttggacacaa actgtttttc
    29281 ggtgacgcgt cgcaccgcct gaatttggtc gcgaatacgt gccgcttctt caaactccag
    29341 attctggctg gcagtttcca tacgactaat gagttgcgta agcacctgat catctttgcc
    29401 agacaaaaac aggcgcacat actcgacctg ctgagcgtat tcttcttcac tcaccagtcc
    29461 ttcaacgcac ggtcccagac agcgccctat ctgatattgc agacacggac gcgagcgatt
    29521 gcgataaaca ctattttcgc actggcgaat ggggaatatc ttttgcagta gcgccagtgt
    29581 ttcacgtacg gcatagccat tcgggaacgg gccgaaatat tcacctttag catgcttcgc
    29641 accacgatgc atcgccagac gcggatgggt atcgccactc aggaagataa agggataaga
    29701 tttatcatcg cgtagcaaaa cgttgtaacg cggctgatag agtttgatgt agttgtgttc
    29761 cagcaacagc gcttcggttt ctgtatgagt aaccgttaca tcaatttgct ggatctgggc
    29821 gaccagcgct tcggttttgc gcgaagcgag gttgctacgg aaatagctgg aaagccgttt
    29881 tttcaggtct ttcgctttgc cgacatagat aaccgtacca ccagcatcgt acatgcgata
    29941 aacgcctggc tggctggtta cggtttttaa aaacgctttt gcgtcaaact gatcactcac
    30001 tgacttgata atgtctccgc attacacaga ccatggcgaa ttgccaggtg agtcagctca
    30061 acatcgccat gaatgtttag tttactgaac atacgatagc ggtagctgtt caccgttttc
    30121 ggactgagat tgagctgttc tgagatctca ttgaccttct ggcccttggt gatcatcagc
    30181 ataatctgca attcacgttc agacaaactg gcaaatgggc tttctgtttt ttctggttcg
    30241 atctggctta acgccatttg ttgagcgatg tcagaagcaa tgtaacgctg ccctgaatag
    30301 acagaacgga tcgcactcac aacttcctgc ggagccgcgc ctttgctgag gtagcccgca
    30361 gcaccggcct gcatgacttt cgctggtaaa gggttttctg tatggacggt aagcatgatg
    30421 attttaacat cagctgtgga acgcgcgatt ttacgcgtcg cctcaaggcc gccaatgccc
    30481 ggcatactca tgtccattag caccacgtca acggcatttg cccggcacca tttaacggcg
    30541 tcttcaccgc acgatgcctc tccgacgact tttataccct ttatatcttc cagaatgcgt
    30601 cgtatccctg cgcgcaccag ttcgtggtca tcaacaagta gaacgttgat caaaggaata
    30661 tctccagaaa tagggataac gctactgata gttagtcatt cgtatattag cggtttttat
    30721 tgcaactttg aaacgttaaa aatggtgggt attcgatttt tctctcgttt ttgtaaattc
    30781 gattgtccac agttatggtc acgcccgtca ccaacggctt ccgggatcgt ttcactgtaa
    30841 gccctgcgat gaaatgtgac aaaaattgac tttattcagc aaaaatgaaa atcagcccgc
    30901 tgaatatgta aca
//
LOCUS       DAZKGF010000037        30486 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZKGF010000037 DAZKGF010000000
VERSION     DAZKGF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.037"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..90)
                     /locus_tag="ACHOQV_001669"
     CDS             complement(<1..90)
                     /locus_tag="ACHOQV_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HFP9630058.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVW"
     gene            complement(83..571)
                     /gene="hybE"
                     /locus_tag="ACHOQV_001670"
     CDS             complement(83..571)
                     /gene="hybE"
                     /locus_tag="ACHOQV_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFP9630059.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMLLSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(564..1058)
                     /gene="hybD"
                     /locus_tag="ACHOQV_001671"
     CDS             complement(564..1058)
                     /gene="hybD"
                     /locus_tag="ACHOQV_001671"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFP9630060.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1058..2761)
                     /gene="hybC"
                     /locus_tag="ACHOQV_001672"
     CDS             complement(1058..2761)
                     /gene="hybC"
                     /locus_tag="ACHOQV_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFP9630061.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(2758..3936)
                     /gene="hybB"
                     /locus_tag="ACHOQV_001673"
     CDS             complement(2758..3936)
                     /gene="hybB"
                     /locus_tag="ACHOQV_001673"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFP9630062.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSAMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICTYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(3926..4912)
                     /gene="hybA"
                     /locus_tag="ACHOQV_001674"
     CDS             complement(3926..4912)
                     /gene="hybA"
                     /locus_tag="ACHOQV_001674"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFP9630063.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGSGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(4915..6033)
                     /gene="hybO"
                     /locus_tag="ACHOQV_001675"
     CDS             complement(4915..6033)
                     /gene="hybO"
                     /locus_tag="ACHOQV_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFP9630064.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6222..6509)
                     /gene="yghW"
                     /locus_tag="ACHOQV_001676"
     CDS             complement(6222..6509)
                     /gene="yghW"
                     /locus_tag="ACHOQV_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFP9630065.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKYCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(6628..7515)
                     /gene="yghX"
                     /locus_tag="ACHOQV_001677"
     CDS             complement(6628..7515)
                     /gene="yghX"
                     /locus_tag="ACHOQV_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFP9630066.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQSYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            7672..8712
                     /gene="gpr"
                     /locus_tag="ACHOQV_001678"
     CDS             7672..8712
                     /gene="gpr"
                     /locus_tag="ACHOQV_001678"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFP9630067.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNAIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(8752..9246)
                     /gene="yqhA"
                     /locus_tag="ACHOQV_001679"
     CDS             complement(8752..9246)
                     /gene="yqhA"
                     /locus_tag="ACHOQV_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFP9630068.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9437..10321
                     /gene="yghA"
                     /locus_tag="ACHOQV_001680"
     CDS             9437..10321
                     /gene="yghA"
                     /locus_tag="ACHOQV_001680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP9630069.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAITDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     ASYVTAEVHGVCGGEHLG"
     gene            complement(10360..10785)
                     /gene="exbD"
                     /locus_tag="ACHOQV_001681"
     CDS             complement(10360..10785)
                     /gene="exbD"
                     /locus_tag="ACHOQV_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFP9630070.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTV
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(10792..11526)
                     /gene="exbB"
                     /locus_tag="ACHOQV_001682"
     CDS             complement(10792..11526)
                     /gene="exbB"
                     /locus_tag="ACHOQV_001682"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP9630071.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            11778..12965
                     /gene="metC"
                     /locus_tag="ACHOQV_001683"
     CDS             11778..12965
                     /gene="metC"
                     /locus_tag="ACHOQV_001683"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFP9630072.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLLPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13105..13764
                     /gene="yghB"
                     /locus_tag="ACHOQV_001684"
     CDS             13105..13764
                     /gene="yghB"
                     /locus_tag="ACHOQV_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFP9630073.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(13804..14703)
                     /gene="yqhC"
                     /locus_tag="ACHOQV_001685"
     CDS             complement(13804..14703)
                     /gene="yqhC"
                     /locus_tag="ACHOQV_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFP9630074.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            14897..16060
                     /gene="yqhD"
                     /locus_tag="ACHOQV_001686"
     CDS             14897..16060
                     /gene="yqhD"
                     /locus_tag="ACHOQV_001686"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP9630075.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16165..16992
                     /gene="dkgA"
                     /locus_tag="ACHOQV_001687"
     CDS             16165..16992
                     /gene="dkgA"
                     /locus_tag="ACHOQV_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFP9630076.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17192..18118
                     /gene="yqhG"
                     /locus_tag="ACHOQV_001688"
     CDS             17192..18118
                     /gene="yqhG"
                     /locus_tag="ACHOQV_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFP9630077.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQPD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQTEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTTNNPDEDM
                     RIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18169..18426
                     /gene="yqhH"
                     /locus_tag="ACHOQV_001689"
     CDS             18169..18426
                     /gene="yqhH"
                     /locus_tag="ACHOQV_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFP9630078.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSDANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(18468..20687)
                     /gene="ygiQ"
                     /locus_tag="ACHOQV_001690"
     CDS             complement(18468..20687)
                     /gene="ygiQ"
                     /locus_tag="ACHOQV_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFP9630079.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            complement(20798..22210)
                     /gene="ftsP"
                     /locus_tag="ACHOQV_001691"
     CDS             complement(20798..22210)
                     /gene="ftsP"
                     /locus_tag="ACHOQV_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFP9630080.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPTLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(22285..23022)
                     /gene="plsC"
                     /locus_tag="ACHOQV_001692"
                     /gene_synonym="parF"
     CDS             complement(22285..23022)
                     /gene="plsC"
                     /locus_tag="ACHOQV_001692"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFP9630081.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(23256..25514)
                     /gene="parC"
                     /locus_tag="ACHOQV_001693"
     CDS             complement(23256..25514)
                     /gene="parC"
                     /locus_tag="ACHOQV_001693"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP9630082.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVAGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(25652..27259)
                     /gene="ygiS"
                     /locus_tag="ACHOQV_001694"
     CDS             complement(25652..27259)
                     /gene="ygiS"
                     /locus_tag="ACHOQV_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9630083.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGIKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(27368..27850)
                     /gene="ygiV"
                     /locus_tag="ACHOQV_001695"
     CDS             complement(27368..27850)
                     /gene="ygiV"
                     /locus_tag="ACHOQV_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9630084.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(27903..28295)
                     /gene="ygiW"
                     /locus_tag="ACHOQV_001696"
     CDS             complement(27903..28295)
                     /gene="ygiW"
                     /locus_tag="ACHOQV_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFP9630085.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            28447..29106
                     /gene="qseB"
                     /locus_tag="ACHOQV_001697"
     CDS             28447..29106
                     /gene="qseB"
                     /locus_tag="ACHOQV_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFP9630086.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGDPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            29103..30452
                     /gene="qseC"
                     /locus_tag="ACHOQV_001698"
     CDS             29103..30452
                     /gene="qseC"
                     /locus_tag="ACHOQV_001698"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFP9630087.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHTMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQANID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
BASE COUNT         7446 a         8159 c         7742 g         7139 t
ORIGIN      
        1 ccacacggcg gtgacgcgct taacatcgtg ctgctccgcc tgccgttgga taatttcaac
       61 ggcgctctgg caaagcgaca actcatgcat tttcgccact cctgcggcca aatagcaggg
      121 cacgacgccc tgcgtgtggt acatccggat tggtgactgg cagcgaaagg agcattcggg
      181 cgcagtcatc cgtcagccgt tggccctctt caatcgacat gctgtgcgaa agcggcgaca
      241 tcagcgagca ggagaggtat tgcgaaacac catccagttc accaacagta aaggtcatag
      301 tgccatacgg cagttgcaga ccaatttttt cactcacttt gcgcagcggc cagagttgat
      361 ccgggccggg gaagataact gcactcagca tccacggggt gatcacacac cccgtccact
      421 gaccttcgaa cagcgtaaag tcagaaacat acaccggcat tgaaggatgc agaaaagaga
      481 gatcatgcat cgaacgccgg gcaatttctt caaaagctgc ctgtacttgc gccttcgggg
      541 aggtctggaa acctgctatc tcttcagtca tgaatcgcct cccgtgggat ggcttccacg
      601 cctgattcac gcagcgcagc cagaacctgc tcaagcgcag gttcaatcat cgcttcgacc
      661 gtcggcgtca agccaatgtg cggctccagt gattccggga tcacgccgac cagggtcagc
      721 tttttcggaa actcaccggt gaagcgcagg gccgacaata cgtcggccag gccaagctga
      781 tgcggagaga ttttgttggt aaacaacgcc ggaacttctt catctcgcag gatcatcatc
      841 gttcccggcg cgttcttttt cgagacaatg gcatccgcaa taatcagatg atcgcgattt
      901 gccatgtcgc caagcagctc cattcccgcc gttccgccat cgaggatctc aacataatcc
      961 ggcagaatgt atcgttgctc taacgcttca acaattcgca caccgatggc ttcatcggtc
     1021 agcaaaatat tgccaacccc taagactaaa atacgcatta cagaaccttc actgaaacca
     1081 cttcgttgcc gtcggcatcc actacgtgta ccgcacaggc catgcacggg tcgaaggagt
     1141 gaatggtacg caccacttcc agcggtttat tcggatcggc aaccggtgta cccaccagcg
     1201 actgctcgta aggaccgacg tcatcattga agttacgcgg gccagagttc caggttgatg
     1261 gaacaactgc ctggtagtta ctgataatac cgtctttgat caccatccag tgagagagca
     1321 taccgcgcgg tgcttcaagg aaaccaacgc ctttgaactc acctgtagcc ggaatgttcg
     1381 gcttcacaaa ggtggtgtga tcgcctttgc cgatattggt gatcagtgca ctgtattgat
     1441 tttgcaggat atcctgcaat tcgcagcagt gaacggtacg accaataata cggcccagcg
     1501 tggagtgcag ctgcgccact tccagcgtgt tgccagtcag tttctgataa atcgcaacga
     1561 tttcattcag tttgttctgg gtagactcgc gtcctgccgc cagtttcacc agcatgttag
     1621 ccagcggacc cacttctacc gttttgccgt agaaagtcgg tgatttcacc caggaatatt
     1681 taccgtcgtc agaccaacca tcataagccg gaatggtggt accttcccac ggtgcctgcg
     1741 gcgcttcgtc tttataccag gagtgcttcg cgctctcctg aatgccttta atcaggtatt
     1801 catcggagtg ggaagtgatc gggcgatacg aggacagatc cgcattctca atgtagccgc
     1861 ccgggaacag gaagctgccg tttttgctgt cggtcgggaa ttccggcacg ctcaggtagt
     1921 tcaccgcacc tttaccgcgc gtcagccatt ccgggtagaa cgcggcgata actgcggtat
     1981 caaccttgta aacctgctca acaaagtcgc tcagtttatc gatgaaagac ttgatgtaca
     2041 tcaggcgctc aaggttcagc acgcccagac cgtcgaggtt gatcgggtta gcgaccccac
     2101 ctactgccag gttctgaatg tgcggcgttt taccgcccag cagcgccacg acgcggttag
     2161 cgtcacgctg gcactccagc gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg
     2221 gcggcagttt catcgccggg tgaccccagt agccattggc gaaaatcccc aactgaccgc
     2281 tggcaaccag atctttaatc ttgttctgaa ctttggtgaa ctcttccgga ctgttcagat
     2341 gccaggtcga aacgcctttc agcatttcag aggctttggt tgggtcagct tgcagtgcag
     2401 aagtgatgtc cacccagtcc agcgccgaga gctgatagaa atgaacgata tggtcatgcg
     2461 tggtgtgcgc agccagaatg atgttacgga tgtattgcgc gttaaccgga acgtcgatat
     2521 tcagtgcact ttctgccgca cgaacggaag agagcgcgtg agtagtcgta catacgccac
     2581 agatacgttg cacaatcatc catgcatcgc gcggatcgcg gtttttcacg atctcttcca
     2641 tgccgcgcca catggtaccg gaagcccatg ctttcgaaac gacgccattt tcgatttcgc
     2701 aatcgatgcg taaatgcccc tcaatacggg ttaccggatc aatagtaatt ctctggctca
     2761 tgctttgctc gcctcatgac gattatgatc gttttgtttt aaaggaggaa gtatcggcag
     2821 tagacgaatg agtacgatgt aagtgcaaat ctcaatagcc acaaaaccaa tagaaatcaa
     2881 cagttcttcc caggtcggga agtaggcgta accgccgccc gggttgaatg ccaccagcga
     2941 ataggtcaga cgccaggttg cacaacctaa cagcgcgctc agtgctgaca ggaacagcat
     3001 gcgggaatca ttacgcagct tcgccacacg cagaacgacc agcgggaaga gcatcagcag
     3061 gacttcaatc cagaacatcg cggagtagaa gtcaccggca aacgctaacg acagcttgtc
     3121 gcgatagata agctcgccaa agcgcagcac gatgaaaatc gccagcaaca cactgatggt
     3181 gttggtcagc ttgacgaaca ggctcttttc atctggacca ttgccacgca gacccgcctg
     3241 caccaacgaa ccttcaaaga tgacaatcga gaagcccatg atgaacgccg tcagcagcga
     3301 gaacagcggc aacatttcat agctctgcca caacggatgc actttgtagc ccgccgagat
     3361 catcagcgac cccattgaag actggtgcat agttggcagc agcgcaccga gcgcgataat
     3421 gaagaacatc accttgttca gacgctgtag cgacaccttc cagcccaatc gttcaaacaa
     3481 tgccggagca aactccagtg ccatcacgcc gatgtagatg gtcatacaga ccgccgtctc
     3541 gaacagtacc gagttcacgt tgaagtgacc cggaatgtag aagtacggca ggttccagta
     3601 gcgaccaacg tcgatggtga tcgacaagcc acccagtgag taaccgaaca gactcgccaa
     3661 cagcgccgga cgcaccagcg gatggtattg cccacggtta aagacgtata ccgcccacgc
     3721 cagcgcccag ccgccacagg caaagccggt gccgatcaac aggtcaaacg cgatccacac
     3781 gccccacggg aagccgccgt tcaggtcaga gacagagccc agaccgaaca ccagacgctt
     3841 cacaatcagg agcatacaga tgacgattaa cggtccaaaa atcatgaccg gtttactgat
     3901 gattttgccg cccagcggtt gtggatcatg actcatgatc gtctcctccg tcgtgatggt
     3961 cgtttttggt gttgcgacga accagcacgg ttaagcccgc cagcacagcc agtggtagca
     4021 tcatgccttt atacagggtg tgttgaatat tttcggaacg cgcaccggta gaaagatcgt
     4081 ccagtttcgg caggtcgaga ttttcgtaag gcacaccggt cagtaccaga acctgagtac
     4141 cgccgccctc tttctcgccg tacagatgcg gataatattt cggcaccgta tgcaggtaag
     4201 tgtcgccaga tttcagcgtc tgacgcggat agtggtattc gctgcctggc ttcagcgcca
     4261 gacgtttttt cgcctcagcc atcagctctt cacgcgtacc aaaaatcacc gcgcccgccg
     4321 ggcacacttc tacgcagcca ggcagaccgc ctttatcaag acgttccaca cctttctggt
     4381 tgcacagttc acacttatgc agcgcaccaa atgggttgtt gtagtcgtac ttcggcacgt
     4441 tgtatgggca ggcgaccatg cagtaacggc agccagtgca cacatctttg tcgtaatgga
     4501 caatgccggt tttcggatct tttttcagcg cagagaccgg gcacacagag acacaattcg
     4561 gatcgacgca gtgcatacac tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt
     4621 tgttgacccc cgagccgctg gtccacacct gaatgatgtt attggtgtac ggcgacagtt
     4681 tgtcgttgtt cgaccaggtc tgttcccctt gcgggttacg ttcagggaaa ttgatatcct
     4741 gacacttggt gacgcaagcc tggcagccaa cgcacaaggt cgagtcgtac aacatcccca
     4801 gcgatcccgg aattggcggg cggttttcag cagccgcatg actgacagac ggcagcgcgc
     4861 ccgtcagcaa tgccccgcag gaggctgctt taataaaatt acgtctgttc acggttattc
     4921 tccccgtgag tcagcgttat ctttcttttg ctgacgaccc agttcacgca ccgccatcac
     4981 gctgacaccg gcaaccagcc caaccacacc gccgagcaaa ccaatagcgc ccgcagagac
     5041 gttgccgcct tctttagcgt taacgtccgg tttctgtgaa cgcggagtct gattttcgac
     5101 gttggcaagc tgatggatgc ctttatggaa gccgatacct tcttcgttac agccatagca
     5161 cgggtgacca atcgctaccg gccacacgcc gccaacgtcg cagaattgca gcgttgagca
     5221 gttgccgtat gtttctggcc ctttacagcc gaggtggtac aggcaccagc cttcgcggtg
     5281 gccttcatca ccgaactctt tggcaaaacg accagcatcg aagtgcgggc gacgttcgca
     5341 gtgttcgtga atcagacggc cataggcgaa ggtcggacgg tttttgtcat ccagtttcgg
     5401 cggcttgccg taagtgatga tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg
     5461 gcagcccgga atattgataa cggttttgcc tggcagaact tcttgcaggc tgactgcgcc
     5521 agttgggtta actccagcgg cggcaacacc gccccacgca gagcaggaac cgatagcgat
     5581 aatggctgct gcgccttccg ccgctttgcg gatgtgatcc acaatcggct caccggcaac
     5641 catgcaataa ataccgttat cttttaatgg gatggaacca tccaccacta acacatactg
     5701 ccctttgtac ttctcgagag cgttatgttt gttctcttcg acctggtgac cgaaagcagc
     5761 agaaagcacc tcgtgatact ccagagagat agtctccagc acgaggtttt ctaccgttgg
     5821 atgcgttgca cgaagcagag attccgtaca accggtgcac tcctgcgcgc caatccagat
     5881 aactggcgga cgctgcgggt tagtaaccga ttcggccatc tctgcagcgg ctttgctact
     5941 taaccccatg gtggcggcta atgctgcaca aagcttcatg aaatcacgac ggttaatgcc
     6001 gtgagaatgg atgagggtgt tatctccagt catttatagt tattccgttg cgaagacctg
     6061 gcatatattt tgcctcaatc gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca
     6121 tttattatgg ttatcgatat catgatactg cggcggggcg ctaaaacgaa gggaagaagc
     6181 attagtgtaa ttaattaaag caagataata cgtatgtttg atcaattttc gaggcgataa
     6241 ccggccataa aaaagcgcaa cgtagaacag gaattattct cacgaaagaa atccattacc
     6301 atctctttat ccagtccata gcggcgcgtc agaatacccg cttcccaggc gctaagctgg
     6361 cgatctccgg tcttttcgta catccggtgt gagtagccta atacaaaacc gcgtttataa
     6421 tcggcacagt attttgtcac attaaccgca ctgtcggcat gcgttgcttt taatcccgcc
     6481 attaagcctt taccaaaatg gttattcatc gttcacctca ttcgctatat attgccgtat
     6541 atagcgatat tttaggcaaa gaacgacatt tgcgcacgct attttttcat gcttgtttaa
     6601 gacgtaatcc tgtaagagat aaacccatca ggagagatat ttatcgaacc atttcagcgt
     6661 cctttgccag gcaagatcgg cggcagattt gtcataacgg ggcgtggaat cattatggaa
     6721 tccgtgatta acccccggat atatatacgc ctcatagacc ttattattgg ctttcaacgc
     6781 cgcctcgtaa gcaggccagc cctcgttgat tcgggtgtcc agttccgcgt agtgcaacag
     6841 taaaggcgct tcaatcttcg ccacatcggc agtgggtgcc tgacgaccat aaaacggcac
     6901 cgcgcaggcc agttccgggt aagcgaccgc tgccgcgttc gatacgccac cgccatagca
     6961 aaatccagta atacccactt tgcctgtcgc ttgcggatag ctttgcataa actcaatcgc
     7021 ggcaaagaaa tcattcatca gtttggttgg atcaacctgc tgttgcagct cacgaccttt
     7081 atcatcatta cccggataac ctccaacgga acttaagccg tcaggtgcca gggcgatata
     7141 ccccgccttc gccactcgcc gcgtcacatc ttcgatatac ggattcagcc ctcgattctc
     7201 atgcaccact accacggctg gcgttttgcc gctcattttt gcgggcttca ccagataacc
     7261 ccgtacctcg ccgtgaccat ttggcgaggg atacgtgatg tactctgcaa caatttccgg
     7321 gtcggtaaac tctacctgag tcgccagcgc ataatttggc ttgagcaaat caaacagcgc
     7381 taatgccgtc atcccgccca ccgcatactt cgccgcaagg ttgaggaact cacgtttcga
     7441 gattttcccg tgagcgtaat agtcgtagta atccagcaac tcttgtggga aatctttggc
     7501 ggttaaacgc ggcatcgttg cactcctcaa ctggtgtttt tttaagcaaa gcataagcgc
     7561 gtgtttttgc ccagtttttc gtcactctgt gagccagact acgggatacg cgctggcgaa
     7621 tcgctaaact agaaacattg tttcgaaatt gaacgatgga aaggagaggt catggtctgg
     7681 ttagcgaatc ccgaacgtta cgggcagatg cagtaccgct attgcggaaa aagtggttta
     7741 cgcctgcccg cgttatcgct cggtttatgg cacaatttcg gtcacgttaa cgcgctggaa
     7801 tcacagcgtg cgatcctgcg taaagcgttt gatttaggca ttacgcactt tgatttagcc
     7861 aacaattacg ggccgcctcc aggaagcgca gaagagaact ttggtcgcct gctgcgcgag
     7921 gattttgccg cttatcgcga tgaactgatt atctctacca aagccggata cgatatgtgg
     7981 cccggcccgt acggctctgg cggctcacgt aaatacctgc tcgccagcct cgaccaaagc
     8041 ctgaagcgta tggggcttga gtatgtcgat atcttttact ctcatcgcgt cgatgaaaat
     8101 acgccgatgg aagaaaccgc ctctgcgctg gctcatgcgg tacaaagcgg taaggcgctg
     8161 tatgtcggga tctcctctta ctcgccagag cggacgcaaa aaatggttga gttgctgcac
     8221 gagtggaaaa ttccgctgtt aattcatcaa ccttcgtaca atttactgaa ccgctgggtg
     8281 gataaaagcg gcctgctgga taccctgcaa aataacggcg tgggctgcat tgcctttact
     8341 ccactggctc agggattgct gaccggaaaa tatctcaacg ctattccgca agattcacgg
     8401 atgcatcgtg aagggaataa agttcgtggt ctgacgccga aaatgctcac cgaagccaac
     8461 ctcaacagcc tgcgcttatt gcatgaaatg gcacagcagc gtggacaatc aatggcgcaa
     8521 atggcgttaa gctggttgct gaaagatgag cgagtgacgt cggtattgat tggagccagc
     8581 cgcgcggagc aacttgagga gaacgtgcag gcgctgaata atctgacatt tagcaccgag
     8641 gagctggcgc agattgatca gcatatcgcc gatggagagc tgaatctgtg gcaggcgtct
     8701 tccgataaat gacctgttaa taacgggcgc gagaaccgcg cccgaatcag atcagtgatt
     8761 atgacgagtc agtcggtcaa gatagcccat cacaaatgca gagagcacaa acgtcagatg
     8821 gataatgacg taccacatca gtttgttatc agggacattt ttcgcatcca taaagacgcg
     8881 cagtaagtga atggaagaaa ttgccacaat cgacgctgct actttgtttt tcagcgacgt
     8941 tgcgtccatt ttccccagcc agttcagctt ctctttgttc tcagagatat cgagttgtga
     9001 gacgaaattc tcgtaaccgg aaaacatcac catcaccagc aaaccgccaa ccagcgtcat
     9061 atccaccagc gacagcaaca cgaggatcaa atctgattcc gccatcgaga agatattcgg
     9121 cagtacgtga ataatctcct ggaagaactt cagcgccagg gcaactaacg ccagcgaaag
     9181 gccaaagtac acgggggcaa gcagccagcg agaagcatac attgcatttt caagaaaacg
     9241 ttccatacat tcctgtcgtg tagttaaaac gggcaactgt atatcgcaat taagtaaaca
     9301 gcacgcaaca gcaaatcaac atatacgtta aacctttgtc gcataattct gagggttagg
     9361 gatcttcctg gttcccttat caacgcatcg agagcgtcta cccttaaagg acgttgaacc
     9421 tgaagggaga aaaatgatgt ctcatttaaa agacccgacc acgcagtatt acactggtga
     9481 atatcccaaa cagaaacaac cgacgccagg catccaggcg aagatgacac cggtaccgga
     9541 ttgcggcgag aaaacctatg ttggtagcgg tcgcctgaaa gatcgtaaag cactggtgac
     9601 agggggcgat tccggaatcg gtcgcgctgc cgccattgct tacgcgcgtg aaggggctga
     9661 cgtggcgatc agttatcttc ccgtggaaga agaagacgct caggatgtca aaaagatcat
     9721 tgaagaatgc ggacgcaaag ccgttctgct gccaggcgat ttaagcgatg agaaattcgc
     9781 ccgttcgctg gttcacgaag cacacaaggc gttaggcggg ctggatatta tggcgctggt
     9841 cgccgggaaa caggttgcca ttactgatat tgcagacctc accagcgaac agtttcaaaa
     9901 gacctttgcc attaacgttt tcgcgctgtt ctggctaacc caggaagcga tccccctgct
     9961 accgaaaggt gccagtatca tcaccacttc gtcaatccag gcataccagc caagcccgca
    10021 tttactggac tatgcggcta cgaaggcggc aattctgaac tacagccgtg gcctggcaaa
    10081 acaggtcgcg gagaaaggta ttcgggtgaa tattgtcgcg ccaggcccga tctggacagc
    10141 actgcaaatt tccggcggac aaacgcagga taagatcccg cagtttggtc agaaaacgcc
    10201 gatgaagcgt gcggggcaac cggcggaact ggcccctgta tatgtttatc tggcaagtca
    10261 ggaggcgagc tacgtcaccg cagaagtgca tggcgtgtgc ggcggcgagc atttaggcta
    10321 aaaaaatgcc cggttgtgaa aagcaaccgg gcatcatttt tatttcgctt tggcagtttc
    10381 ttcgccgacc agacctatct tcaggtaacc cgcctgatgc agcgtatcca ttaccttcat
    10441 caacgtctcg taatcgacgg ttttatccgc tcggaagaag acggtggtgt ctttcttgcc
    10501 ttcggttaac gcattcaacg ccgtaatcat tgtttcatcg gtgaccggat cgttaccgat
    10561 aaacatcgag ttgtctgcct tcaccgacag ataaaccggt ttttccgggc gcggctgcgg
    10621 cgtgctggta gaagcaggca agttcacctt cacatctacc gtcgctaacg gtgccgccac
    10681 cataaagata atcagcagaa ccaacatcac gtcgataaac ggcgtcacgt tgatatcatg
    10741 catttcgccg ttatcgtcga ggttttcgtt aagatgcatt gccatcggac attatcctgc
    10801 gcgtaatttt tgtgcgacac gaaccggatg cgcagcggcg ctggcttcca gatccaggtc
    10861 acggctttgc agcaacaata cctgcgctgc aacatcaccc agcatcgctt taaagccgcc
    10921 aatctggcgt gcaaatacgt tatagataac gaccgccgga atcgctgcca cgaggccgat
    10981 tgccgttgct aacagagctt ctgcgatacc cggcgcaacg actgccaggt tagtagtctg
    11041 cgtttgcgcg ataccaataa agctgttcat gatgccccag accgtaccaa acagaccaac
    11101 gaacggcgaa atcgcgccga tggttgccag atagccgtta ccacggccca tttgacgacc
    11161 tactgcggcg acccgacgct ccagacggaa gctggtacgt tctttaatac cttcgttatc
    11221 gtcgctgcct tctgacagtt ccagctcatt ctgcgcttca ttgagcaaat gcaggctcaa
    11281 gcttttgcta ccaaaatcag cggcgatatc gttggcctgg tttaaggaac gcgcttcagc
    11341 cagcagttgc tgctcgcgct taagacgacg cttctgattg aagaactcca cgctcttact
    11401 gaagaagatt gcccaggtga ctacggaggc caaaataagc ccaatcatca cgcacttaac
    11461 gacaatatcg gcgtgctgat acatacccca gacggagagg tccgtctgca ttaaattatt
    11521 acccactgtg tatctccagg acgcaagtca caaaatctgc gcataataat atcaaaacga
    11581 cgtcgaattg atagtcgttc tcattactat ttgcatactg ccgtaccttt gctttctttt
    11641 ccttgcgttt acgcagtaaa aaagtcacca gcacgccatt tgcgaaaatt ttctgcttta
    11701 tgccaattct tcaggatgcg cccgcgaata ttcatgctag tttagacatc cagacgtata
    11761 aaaacaggaa tcccgacatg gcggacaaaa agcttgatac tcaactggtg aatgcaggac
    11821 gcagcaaaaa atacacgctc ggtgcggtaa atagcgtgat tcagcgcgcg tcttcgctgg
    11881 tctttgacag tgtggaagcc aaaaaacacg cgacacgtaa tcgcgccaat ggcgagttgt
    11941 tctatggacg gcgtggaacg ttaacccatt tctccttaca acaagcgatg tgtgaactgg
    12001 aaggtggcgc aggctgtgtg ctatttccct gcggggcggc agcggttgct aattccattc
    12061 ttgcttttgt cgaacagggc gatcatgtgt tgatgaccaa caccgcctat gaaccgagtc
    12121 aggatttctg tagcaaaatc ctcagcaaac tgggcgtaac gacatcgtgg tttgatccgc
    12181 tgattggtgc cgatatcgtt aagcatctgc tgccaaacac taaaatcgtg tttctggaat
    12241 cgccaggttc catcaccatg gaagtccacg atgttccggc gattgttgcc gccgtacgca
    12301 gtgtggtccc ggatgccatc attatgatcg acaacacctg ggcagccggt gtgctgttta
    12361 aggcgctgga ttttggcatc gatgtttcta ttcaggccgc caccaagtat ctggttgggc
    12421 attcagatgc gatgattggc actgcggtgt gcaatgcccg ttgctgggag cagctgcgag
    12481 agaatgccta tctgatgggc cagatggtcg atgccgatac cgcctatata accagccgtg
    12541 gcctgcgcac attaggtgtg cgtttgcgcc aacatcatga aagcagtctg aaagtggctg
    12601 aatggctggc agaacatccc caagtagcgc gagttaacca ccctgccctg cctggcagta
    12661 aaggccacga attctggaaa cgagacttta caggcagcag cgggctattt tcctttgtgc
    12721 ttaagaaaaa actcaatgat gaagagctgg cgaactatct ggataacttc agtttattca
    12781 gcatggccta ctcgtggggc gggtatgaat cgttgatcct ggcaaatcaa ccagaacata
    12841 tcgccgctat tcgcccacaa ggcgagatcg attttagcgg aaccttgatt cgcctgcata
    12901 ttggtctgga agatgtcgac gatctgattg ccgatctgga cgccggtttt gcgcgaattg
    12961 tataacattg ccacttttgg acaattttgc agacatttta ttgtgaaaag tcttaaattg
    13021 ttgcgtccgg gatcaaggcg tcccggacga ttcaggagta caataggcag ataaaggctt
    13081 aaacgctgtt ccacaggaaa gtccatggct gttattcaag atatcatcgc tgcgctctgg
    13141 caacacgact ttgccgcgct ggcggaccct catattgtta gcgttgttta ctttgttatg
    13201 tttgccacgc tgtttttaga aaacggcttg ctgcccgcct catttttgcc aggcgacagc
    13261 ttgttgatac tggcaggcgc attgattgcc cagggggtta tggattttct gcctacgatt
    13321 gcgattctga ccgccgcagc aagtctgggc tgctggctaa gttatattca ggggcgctgg
    13381 ttagggaata ccaaaacggt gaaaggctgg ctggcacagc ttcctgctaa atatcaccag
    13441 cgcgccacct gcatgtttga ccgccacggt ctgctggcgc tgctggctgg acgttttctt
    13501 gcatttgtcc gtacgctgct gccaaccatg gcgggaattt ccggtctgcc aaaccgccgc
    13561 ttccagtttt tcaactggtt aagcggattg ctgtgggtca gcgtggtaac cagtttcggc
    13621 tatgccttaa gtatgattcc gttcgttaaa cgccatgaag atcaggtaat gacgttcctg
    13681 atgatcctgc caattgcctt gttaaccgct ggcttgttgg gcacgctgtt tgtggtgatt
    13741 aaaaaaaaat actgtaacgc ctgacgattt tccccattcc cggtagctgt accgggaacg
    13801 tatttaattc ccctgcatcg cccgcattct tgccgcatct tcccccggcg tcacaccgaa
    13861 gtaacgttta aactcacggc taaattgcga tgcgctttca tagccgacgc gcatcgccgc
    13921 cgcgctggcc ttcatgccgt cgtggatgat catcatccgc gccttatgca gacggtaatt
    13981 cttcaaatac tgcaacggcg aggtgctggt gacagactta aaattatggt ggaacgccga
    14041 tacgctcatg ttggcttctg ccgccagttg ctcgacgctc aggttttcgg tgtatttatt
    14101 ctcaatccgt ttcagcacgc gactaatcag gctgaagtga gtctggcggc tgaccagtgc
    14161 cagtaacgcg ccgccgcaag gtccggtcag cacgtagtac agaatttcgc ggatgatctg
    14221 tttgccgaga atacgcgcat ccagcggtcg ctccatcaca tccagtaacc gctccgccgc
    14281 gcataaaatc tcttctgata acgtggcaga gttaatcccg ctggctgcca tcgacggctg
    14341 gaaatgctca tcttcgccaa tgtccatcaa cagttcctgt aactgcaaaa tatcgacatt
    14401 gagacgcaac cctgccagcg gcacctctga cgtggcatag gtttcgcact caaacggcaa
    14461 cggcaccgtc agcaggagat attcattagc gtcataacga aacacgcgtt cattgatata
    14521 accgatttta tgtccggaaa agagaattat gatgccaggc tcgtacatca ccggtgtgcg
    14581 tgcgaaaggc gtctcgccat acaacaaacg cacatcgggc aacagctctg acaaactatt
    14641 ttctttattt ttcagtttat taactttatc cgccagcaag cggcaaatct cttcacgttt
    14701 catatcgcgt aatttcttag gaataatgcg gcaatttgat tgtgcgcaat tttgtagcat
    14761 ttctccagca ctctggagaa ataggcaaga cattggcaga aatgagcatt gagaggtacg
    14821 gcgctggcga ccacaatgaa aaacatcagg cagaccgttc tctgccctca tattgaccca
    14881 gcaaagggag caagtaatga acaactttaa tctgcacacc ccaacccgca ttctttttgg
    14941 taaaggcgca atcgctggtt tacgcgaaca aattcctcac gatgctcgcg tattgattac
    15001 ctacggcggc ggcagcgtga aaaaaaccgg cgttctcgat caagttctgg atgccctgaa
    15061 aggcatggac gtgctggaat ttggcggtat tgagccaaac ccggcttatg aaacgctgat
    15121 gaacgccgtg aaactggttc gcgaacaaaa agtgactttc ctgctggcgg ttggcggcgg
    15181 ttctgtactg gacggcacca aatttatcgc cgcagcggct aactatccgg aaaatatcga
    15241 tccgtggcac attctgcaaa cgggcggtaa agagattaaa agcgccatcc cgatgggctg
    15301 tgtgctgacg ctgccagcaa ccggttcaga atccaacgcc ggcgcggtga tctcccgtaa
    15361 aaccacaggc gacaagcagg cgttccattc tgcccatgtt cagccggtat ttgccgtgct
    15421 cgatccggtt tatacctaca ccctgccgcc gcgtcaggtg gctaacggtg tagtggacgc
    15481 ttttgtacac accgtggaac agtatgttac taaaccggtt gatgccaaaa ttcaggaccg
    15541 tttcgcagaa ggcattttgt tgacgctgat cgaagatggt ccgaaagccc tgaaagagcc
    15601 agaaaactac gatgtgcgcg ccaacgttat gtgggcggcg actcaggcgc tgaacggttt
    15661 gatcggcgct ggcgtaccgc aggactgggc aacgcatatg ctgggccacg aactgactgc
    15721 gatgcacggt ctggatcacg cgcaaacact ggctatcgtc ctgcctgcac tgtggaatga
    15781 aaaacgcgat accaagcgcg ctaagctgct gcaatatgct gaacgcgtct ggaacatcac
    15841 tgaaggttca gacgatgagc gtattgacgc cgcgattgcc gcaacccgca atttctttga
    15901 gcaattaggc gtgccgaccc acctctccga ctacggtctg gacggcagct ccatcccggc
    15961 tttgctgaaa aaactggaag agcacggcat gacccaactg ggcgaaaatc atgacattac
    16021 gctggatgtc agccgccgta tatacgaagc cgcccgctaa gctttttacg cctcaaactt
    16081 tcgttttcgg gcatttcgtc cagacttaag ttcacaacac ctcaccggag cctgctccgg
    16141 tgagttcata taaaggagga acgtatggct aatccaaccg ttattaagct acaggatggc
    16201 aatgtcatgc cccagctggg actgggcgtc tggcaagcaa gtaatgagga agtaatcacc
    16261 gccattcaaa aagcgttaga agtgggttat cgctcgattg ataccgccgc ggcctacaag
    16321 aacgaagaag gtgtcggcaa agccctgaaa aatgcctcag tcaacagaga agaactgttc
    16381 atcaccacta agctgtggaa cgacgaccac aagcgccccc gcgaagccct gctcgacagc
    16441 ctgaaaaaac tccagcttga ttatatcgac ctctacttaa tgcactggcc cgttcccgct
    16501 atcgaccatt atgtcgaagc atggaagggc atgatcgaat tgcaaaaaga gggattaatc
    16561 aaaagcattg gcgtgtgcaa cttccagatc catcacctgc aacgcctgat tgatgaaact
    16621 ggcgtgacgc ctgttattaa tcagatcgaa cttcacccgc tgatgcaaca acgccagcta
    16681 catgcctgga acgcgacaca caaaatccag accgaatcct ggagtccatt agcgcaaggt
    16741 gggaaaggtg ttttcgatca gaaagtcatt cgcgatctgg cagataaata cggcaaaacc
    16801 ccggcgcaga ttgttatccg ctggcatctg gatagcggcc tggtggtgat cccaaaatcg
    16861 gtcacacctt cacgcattgt cgaaaacttt gatgtctggg atttccgtct cgacaaagac
    16921 gaacttggcg aaattgcaaa actcgatcag ggcaagcgtc tcggccccga tcctgaccag
    16981 ttcggcggct aacgtgcaaa ttctcccggt ggcggtaatg ttccgctacc ggacttttca
    17041 gaaatcattt attcccctcg cgtcctgccc attgttactc ttccttgttc aggaatgcca
    17101 aatataagga catcatcatg cagagccgga agctcttaaa ggaacaactc atctacaccc
    17161 gggataaacg caacggagag gtgaaaaaca gatgaaaata atacttctgt ttttagcagc
    17221 cctggcaagt tttaccgtac acgcgcagcc tccttcacag accgtagaac aaacagtccg
    17281 gcagatttat cagaactata aatcagatgc cagtacgcct tattttggtg aaaccggaga
    17341 gcgagcgata acttctgcgc gtattcaaca ggcgcttacc ctgaacgaca atcttacgct
    17401 gccgggcaat attggctggc tggattatga tccggtttgt gattgtcagg attttggcga
    17461 tctggtgtta gaaagcgtag cgataaccca acctgacgcc gatcatgccg atgccgttgt
    17521 gcgctttcgt atctttaaag acgataaaga aaagaccacg cagacactga aaatggtggc
    17581 ggaaaatggt cgttgggtca ttgacgatat tgtcagcaat catggcagcg tcttacaagc
    17641 agttaatagc gagaatgaaa aaacgctggc cgctatagct tcgttgcaaa aagaacagac
    17701 ggaagccttt gttgccgaac tctttgaaca tattgctgat tatagctggc cgtggacgtg
    17761 ggtggtttcc gactcttacc gccaggcggt caatgccttc tataaaacca ccttcaagac
    17821 taccaacaat cccgatgaag atatgcgaat agaacggcaa tttatttacg acaatccgat
    17881 ctgttttggc gaagagtcgc tattttcacg cgttgatgaa attcgagtcc tggagaaaac
    17941 cgccgattcc gcccgcattc atgttcgttt tacgctgacc aatggcaaca atgaagagca
    18001 agaactggtt ttacagcggc gcgaaggtaa gtgggaaatc gctgatttta tccgcccgaa
    18061 tagcggtagc ctacttaagc agattgaggc aaaaaccgcc gccagattaa agcaatgagc
    18121 tgagttaaat aacaattagc cggaacaata aataaaaggg aacactatat gaaaacgatt
    18181 ttcaccgtgg gagctgttgt tctggcaacc tgcttgctca gtggctgcgt caatgagcaa
    18241 aaggtcaatc agctggcgag caatgtgcaa acattaaatg ccaaaatcgc ccggcttgag
    18301 caggatatga aagcattacg cccacaaatc tatgctgcca aatccgacgc taacagagcc
    18361 aatacgcgtc ttgatgctca ggactatttt gattgcctgc gctgcttgcg tatgtacgca
    18421 gaatgataaa aaaattcccg gcagcattct gttaccgggg atatttttta acgtccaacc
    18481 gccgctttag ggcgtttctt cgcaccagca ttctgcaggc gagattgcgt agacgacgct
    18541 ttttttgccg tagcaggcgt ctgacgctgg gtcgccatcg gcgtatgttt agtcaacgcc
    18601 ggacgggtat tgcggttctg gcgacgagcc tcacgcatct cttcaatggt tggcgcaggc
    18661 actaagcaat cgcgacggct gccaatcaga tgctttttgc ccatcgcttc cagcgcctgg
    18721 cggattaacg gccagtttgc cggatcgtgg taacgcaaca acgctttatg caggcgacgc
    18781 tgcttgtcgc ccttcggtac aaagacgtct tcacttttat aaccaatctt cgccagcggg
    18841 tttttgccgg tgtaatacat agtggtcgag ttagccagcg gcgacgggta gaagttctgt
    18901 acctggtcga ggcggaagcg gtgctttttc agccacagcg ccagattcac catatcttca
    18961 tcgcgcgtac ccgggtgcgc ggagatgaaa tacgggatca gatactgttc tttacctgcc
    19021 tgtttcgagt aagtatcgaa cagctcttta aagcggtcat agctgcccat gcccggcttc
    19081 atcatcttcg ataacggccc ttcttcggta tgttccgggg caatcttcag ataaccgcca
    19141 acgtgatggg tcgccagctc tttgatatag cgcggatctt ctacggcgat gtcataacgc
    19201 acaccagagg caatcaggat ctttttaatg cctttcagat cgcgcgcacg gcgatagagg
    19261 ttaatcgtcg gttcgtggtt agtgtccata tgcggacaaa tatccggata aacgcacgac
    19321 aaacgacggc aggtttgctc agcgcgtggc gatttgcaac gcaacatata catgttggca
    19381 gtaggcccac cgagatcgga aatcacaccc gtaaaacctg gaacagtgtc gcggatcgct
    19441 tcgatctcat taataatcga atcttcggaa cggctctgaa taatgcgccc ttcgtgctcg
    19501 gtaatagaac agaaagagca gccgccaaag cagccacgca taatgttgac cgagaaacga
    19561 atcatttcgt aagccggaat tcgggcatta ccgtaagccg gatgcggcac gcgcttgtac
    19621 ggcagcgcaa agacgctatc catctcttcg gtagaaagcg gaatagcagg cgggttgatc
    19681 catacatagc ggtcgccatg tttttgcatc aatgcgcggg cgcagcctgg gttagtttcg
    19741 tggtgaagaa tgcgcgaagc atgggcgtac agcactttat cgcccttcac tttctcgaaa
    19801 gaaggcagca acacgtaggt tttttcccac ggtttcgggc gcggcggctg cacggttacg
    19861 gctttcgctt cctgcttttt cggcgcaaca ggtttgttat ccgcgcacgg cagatcttcg
    19921 ccatacggat gcgggatcgg gtcgattttc ccaggagtat caagacgggt ggaatccacg
    19981 ccgctccagc ctggcaacgc ctcttttacg ataatcgcgg tattacgcac atcgcggatt
    20041 tcactaatcg gctcgcccat cgccagacga tgcgccactt ccaccagcgg acgctcaccg
    20101 ttaccgaaca tcagcatgtc ggctttcgaa tccaccagca cggaacggcg cacggtatcg
    20161 gaccagtaat cataatgcgc ggtacggcgc agacttgcct caataccgcc gaggatcacc
    20221 ggtacatctt tccacgcctc tttacaacgc tgggtataaa ccagtgtggc gcgatccggt
    20281 cgcttacccg cgacgttatc cggcgtgtag gcatcgtcat gacgtaaacg gcgatcggcg
    20341 gtatagcggt tgatcatcga gtccatgttg ccagcagtaa caccgaaaaa cagattcggt
    20401 ttacccagac gcataaagtc gtctttgctg ctccagtccg gttgggcaat gatcccgaca
    20461 cgaaagccct gcgcttccag catacgaccg caaatcgcca tcccgaagct tgggtgatcg
    20521 acatacgcgt cgccagtaac caaaatgatg tcgcagctat cccagccaag ttgatccatc
    20581 tcttcacgag acatcggcaa aaacggtgcc ggtccaaaac aggcggccca gtactgcggc
    20641 caggagaaca ggtcgcgatc cggttggatc agggagatag agctcatttt gcttccagaa
    20701 atgataaaaa aataatcaaa ggccggggat tataagccgg aacgaaagag aaatcgaaag
    20761 gtattccata ctcgccctcc tcgggcgagt atgaagatta cggtaccgga ttgaccaaca
    20821 gttgccctat cgagccacgg tccgccattt ccagcgtctg actgttgaag tagaacggga
    20881 agtgcgccca ggaaggctga ccgaaataaa ccagcagctc cacttgtccg tcaacccaaa
    20941 cggtatcttt ccagcctctg tcttccggga acggcatcgc gccattcacg ttacggatct
    21001 ggaacatcac gccttcaata tggaacgctt gcggctcgtc cgcgcgtacc gtccagcgtt
    21061 cccacgttcc ttgctgcgcg gtgacatcaa tacggttgac gtcccacagt tgtccattaa
    21121 tacccgggtc atcacccaga ctgatatcgc gactgcgaat cggcgaaccg gccatgattt
    21181 cagttggcag caggcgcatc ggaagactgt ctgtgaccag cggcagaagg ccggttgggc
    21241 gcagcgttag caccagggta gaaaccagaa tgctggatgg ctcaaagaag ccgcgaatac
    21301 gatcaacaat gctcgccgct tcaccacagg tgatcgacac ttcatcaccg ttgctcatat
    21361 ccaccagaat ctcgcggcgc tcgcccggtg ccagcgaaag ttgcttcacc gatacaggag
    21421 cgggcaggaa tccctgatcg ccagaaatca catgtaacgg gcgaccatcg ctcatctgta
    21481 gttgatagcg acgagagttc gacgcgttca acagacgcaa gcgcacccag ccacgcgaga
    21541 cttcaacgta cgggctttgt acaccgttaa ccagcagcgt atcaccaaca aagccgccgc
    21601 ttcccggttc gttatattcc ggcgtaccaa agttatccag ccgtttatcc tgaataatga
    21661 ccggaaaatc atccacacca taatggttgg ggataggcag cgatttgctg acttcatctt
    21721 ccaccagcca cattccggca aggccgttat agacctgctg agccgtgcgg ttgggagtat
    21781 tggcgtgata ccacagagta gctgcgttct ggcgaatggg cagtacgggt gcccagtcag
    21841 cgtttggcga catcatccgt gccggaccgc ccatcagcgg gccaggtacc tgtagcccgg
    21901 ccaccgtcat tgagacattt tctgtcaggc gattgctgta aataagctta acatcgtcgc
    21961 ccttccagac gcggatagtc ggccccaggt aacgaccatt gattccccaa accgatgcgc
    22021 gtgtccctgg cgtaaatgac cagtgcgcac gttgtacagt cataaacagc ggttgcccac
    22081 ggcgagattc aagcagcgtc ggaacgggta gcggttgctg ttgcccggct gcgctggcct
    22141 tcaggggaac agcgcctgca caaagtgcaa tccccgatgc ctgaatgaac tgacgccgac
    22201 tgagtgacat atttgctcca tgtaaaactg gctaaagtaa taccgtgcag gaaagtgttc
    22261 cccgcaaaaa tacagattgc cgacttaaac ttttccggcg gcttcgcgct ctgcgacttc
    22321 tttatcgagc tcagcgattt tttgttccat tatcgaacga caatgggcag ccaactcgcg
    22381 aacctgatct ttgccatact gactgacgtc aattggcggc agcatttcga caatcaccag
    22441 accgttgtgt agccgattaa gattaatctt attcgaagtt gtagagacgc acacgggaat
    22501 aatcgggacg cccgccgcaa ttgccgcgtg aaatgccccg gtcttgaacg gcagcaggcc
    22561 gcgaccacgg ctgcgggttc cttccgggaa catccagatg gaaatacggc gttttttgaa
    22621 gtgattcacg acttccgcaa tagtgccgtg agctttagtg cgattgtttc tgtcgatcaa
    22681 taagttgccg gttaaccagt acaactgccc gaagaagggg atccacagca agctcttttt
    22741 ccctaccgtc actgtcggcg gctgcacaat gttcgatgcc gtcaccatgt cataattgtt
    22801 ctggtggtta gcaatataga tagcatttcc gtagctttca gcatccgcag gtttgcggca
    22861 ctcaactttc aggccgaaca gcggtgcaag acggccaaac atatgcccaa aggtggccac
    22921 atgtttcggg ttacgcgggc tgaaaaggca gtaaatggag ccgaatacac agactaagat
    22981 gctgtaaatc acggtaataa taagacgaaa gatatatagc ataacaccct ctgaaggtct
    23041 gagagactgg cattgtacgt cacctgaaat caggttaggg cattgttaaa agccgctctc
    23101 tggaaaagcg tattgttaat cgcaacgcat gaataaacaa cggtttcaag ggaattttta
    23161 tcgtttccgg gggttgaata cgctgccgga tgacgactta acgtttcatc cggcgttcct
    23221 tgcaagcggg aggaaacagc gccctccccg gcatattact cttcgctatc accgctgctg
    23281 gcacggcgag gagagtcgat ctcaacacga tcgatacgct gcaaaccacg catcaacgta
    23341 ccgcggcgtc cacgttcgcc agcgactttc tgtagctctt ccggacgcag tttaattttg
    23401 cgtttcccaa catgaatggt cagcgtgctt tgcggcggca gaacgtacag ttgcgcaaga
    23461 ccatcctcgc cacgcgcggc ttctgccgat ggaatgttga taatcttgtt gcctttgccc
    23521 ttcgacagct gcggcagatc gcttaccggg aacatcaaca tacggcctgc ctgagtgatt
    23581 gccagcagca tatcggaagc atcttcaatc accaccggcg gcataacatg ggcattttcc
    23641 ggtaaggtga tcaaagcctt acctgcacgg ttacgcgcca ccagatcgtt aaaggtgcag
    23701 acgaaaccgt aacccgcatc ggaagccatc agcagtttct gatcgtcgct ttccatcagc
    23761 atatggtcaa cggtcgctcc aggcggcaac gttaatttgc cggtgagcgg ctcaccctga
    23821 ccacgcgccg acggcagcgt aatcgggtcg atggcatagc tacgaccggt ggaatcaaca
    23881 aacactaccg gttggttgct cttacctttc accgccgctt tgaagctatc gcccgcttta
    23941 taattcaggc ccggcgcgtc aatatcatgg cctttagcgc tgcgcaccca gcccatctgc
    24001 gacagcacaa tggtgacagg ttcagacggc agcatgtcgt gctcgctcat tgctttcgct
    24061 tcttcgcgtt cctgcaacgg cgaacgacgt tcgtcaccgt aggcttgcgc gtctgcctgc
    24121 agttctttct tcagcaggtt attcatttta cgctcggaag ccaaaatgcc ctgcaactgg
    24181 tcgcgctctt tttccagctc actctgctca ccgcgaatct tcatctcttc cagtttggca
    24241 agatgacgca gtttcagttc gaggatcgct tccgcctggg tttcagtaag gccaaaccgc
    24301 gacatcagcg ccggtttcgg ttcatcttca ttacgaatga tctcaatcac ttcgtcgata
    24361 ttgagaaacg ccaccagcaa accttcgagg atatgcaggc gcttgaggac tttctccaga
    24421 cgatagttca gtcggcggcg cacggtatcg cggcggaaca ccagccattc ggagaggatt
    24481 tccagcaggt ttttcaccgc cggacgacca tccagaccga tcatattgag gttaatgcga
    24541 tagctctttt ccagatcggt ggtagcaaag aggtggttca tcacctgatc catatccacg
    24601 cggttggaac gcggcacaat caccagacgg gtcgggttct cgtggtcaga ttcatcgcgc
    24661 agatcgtcaa ccatcggcag ctttttgttg cgcatttgcg cagcaatttg ctccagtacg
    24721 cgcgcacctg aaacctgatg cggcaatgcg ctgataacca ccgcgccatc ttctttcttc
    24781 caaaccgcgc gcatacgcac tgaaccacgt ccgttctcgt agattttacg aatctcggcg
    24841 cgcgaagtga taatttccgc ttcagtcgga taatccggcc cctgcacgat atccagcagc
    24901 tgatcgagcg tggttttcgg ctggtcgatt aatgcgattg ccgcctgagc cacttcacgc
    24961 aggttatgcg gtggaatatc ggtcgccatg ccgacggcaa taccggtggt gccgttaagc
    25021 aaaatgtttg gcagacgggc aggcagcatt ttcggctcct gcaaagtgcc gtcgaagttt
    25081 ggcacccagt cagccgtccc ctgccccagc tcgctcaata gcagctcgga atatttcgac
    25141 aaccgggatt cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg cgcgccccag
    25201 ttcccctgac catcaaccag cggataacgg taagagaacg gctgcgccat caggaccatc
    25261 gcttcataac aggcactatc gccgtgcgga tggtatttac ccagtacgtc accgacggta
    25321 cgggccgatt ttttaaattt ggcgctggca ttcaggccca gctcagacat cgcatacaca
    25381 atgcggcgct gaacaggttt cagaccatca ccaataaacg gcaacgcacg gtccatgatc
    25441 acgtacatgg agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc aaggcgctct
    25501 gccatatcgc tcattaattc tgattcctca acttattcgc cagccttcaa aggggcaata
    25561 ttgccggaga tactacatta tctgccgcgt cgagtcacag gagatcagcg tgaatagcgc
    25621 cgccggacgt gctccggcgg cgataaaggc atcaatgtgc cttgatatac aactctttgc
    25681 tgtagacata atcctgggga ttatgcagcg gaaaaccgcc aacgtagggt ttaagcagtt
    25741 tgattaacgg ctgatagtag atgggaatca gcggtgcctg ctggttgatg atcacttctg
    25801 cctgctgata caacgcatta cgctttgtcg catcagtgat ctgcgtggcc tggtttagta
    25861 aggcgtcata ctgcgcattt ttccagtgac cgacgttttc ttcactatcg cttttgagcg
    25921 tgttcaggaa gctggaggca tcattgtacg tcgcatccca cgactgccga gacagcataa
    25981 aatcaccggc tcgtcgggca tcaagatagg ttttccactc cattgtgcgc agcgtcacct
    26041 gtgcacccag ccattttttc cattcggaag acaacgctat cgcggtcttt tcatgcagat
    26101 cgtacttgtt gtagaacagc tcaaagcgta gcggatgaga ggcgtcgtat cccgcctgtt
    26161 tcagcaaggc ttttgccatc gcgacacgct cactcattgg cttttgcagt tcatcgaacg
    26221 tcgtcgcgct aaagcctttt acctctggcg gcgtcagcgt ggttgcgggc gttctcaacc
    26281 ccagtacctt ttgcgcaata agctgtcgat caaccgtaag atatagcgcc cgacgcaccc
    26341 gcacatcgtt aaatggcggt ttctcaaggt taaagttgta atattcgctg ttcagacgcg
    26401 ggataattcg tagctcgcca ggcagtgatt tttcaatggc gggaatttgc tgcgccggaa
    26461 cccaggtgag atcgacctct cccgcgcgat agcggttata gccggtgacc gaattatcca
    26521 gcgccagata ctcaacctgt tgcaataccg tatgttgcgc atcgcggtac tttggatttt
    26581 tgcgtgcggt aatcttttcg ttaactaccc atttatcaag cacaaaggca ccgttgtaaa
    26641 ccatgttctc tggcttactc cagctatcgc catgtttagc gatgacatga tgaggaaccg
    26701 ggaacaacgt cggccaggcg agcatcgtcg taaaccaagg aactggctgc tcaagcgtaa
    26761 cttcaagagt acgatcatcc gtcgctttga tccccagcga tgtaacatct gctttacccg
    26821 caacaatagc cgcggcattg ttaatgtgcg cctgtgccag atagccagca aaagggcttg
    26881 ccgttttcgg gtcaaccgca cgttgccagc cgaggacaaa atcctctgcc gtcagaggct
    26941 gaccgtctga ccactgcaaa ccgctacgca gatggaaaat atagcgcttg ccgccgtcaa
    27001 gtatctccca gcgttcagcc tgagcgggct gcacctggcc ttcaccgtcc atccatacca
    27061 gaccttcaaa cagatccagc acaatctgcg cggcagtatt ctcctctacc ttttgcgggt
    27121 cgagcgtgcc tgggtcgcta tgattgttgt aacgaaagac ttgttgcggg gcgagcggtg
    27181 tgttggcggg aacgtcagca gcgtagagag gagccgcact taccaggctg accagccata
    27241 ataaatttcg cgtatacatg gtattcctta ccggagaaat tataagcccg acaacaatac
    27301 gcctgtggca ttgtttgctc aaacttatcg cgagtgattt ggctcacact ccggtaaaga
    27361 ttccccatca cgccaacggc acataaatat ccgtttccag tcgccgctct gtcatccctt
    27421 cggcaagatt ggtgtagtga aacaggatcg gggctttgcg cattttttcg ccgcttgcag
    27481 gtagccagtg gcgaatgatg ccccatatcg tgtgtgaaat atcgtccagc tcgccaacat
    27541 ggcgggcaac agcataacgc ccaccggtaa gttcgccatt actcacgcca taacgattat
    27601 cggggatcgg ttcgctaacg ctgccgcaga tatcaaagcg aaacgcttct ggtgcggtgg
    27661 tggctgggtc gtcccaggcg acgccaaaag tctggctttg gttaacagga gaaagccccg
    27721 tctctttacg ccacatgata aatttcgcca cgctgtagtt gagcaactca gggctacagc
    27781 ggtgcggcaa catcgccaca gggattgatg ggaaatcgat aatatttaca tccagtgtca
    27841 ggtttgtcat tttgcctccc tgcgaaatta ttcccgggaa cgcgtcccgg gagcggtaac
    27901 aattacggat ttactttgcg gatctgtttg acgtcaattt caacagagtt ccagtcttta
    27961 tcgacttccc cctgaatctc aaccgtatct ttcggcgtca ccgtcacgcc gttccagcgt
    28021 ttatggtcga tatcaacatt gatagtaccg ctggcatctt tgaacacgta gagatcgtca
    28081 gagatgcgtt caacgatatt gccgcgtagg gttacccagg tgtcgtcacg cagggatttt
    28141 gcgctttcta cagtcgttac gctgccgttc ggcccctgga atcctccggc ctgactttgc
    28201 gttgccgatg ggccagaaaa acctccctgc tctgctgcca taaccggtgc actgcacagg
    28261 gccattactg cgattactgc tgcgaatttt ttcatgttta ttactccctt taatgtctgt
    28321 ttccgagcat ttaacaagat agtccttaac aacttcttaa gggaaaaaaa taaaatttaa
    28381 tgctgtacag agcgcgttac aacacggttt actggcagca aatacggtta tcgcagggat
    28441 gaaaaaatgc gaattttact gatagaagat gacatgctga ttggcgacgg catcaaaacg
    28501 ggccttagta aaatgggttt tagcgtcgac tggtttacac aaggtcgtca gggaaaagag
    28561 gcgctatata gcgcacctta tgatgcggtg atcctggatt taaccttacc gggcatggat
    28621 ggtcgcgata ttttgcgcga atggcgagaa aaaggtcagc gtgagccggt attgatcctg
    28681 actgcgcgcg atgcgctggc ggaacgtgta gaagggctgc gtctgggagc tgacgattat
    28741 ctgtgtaaac cttttgcgtt gatagaagtc gccgccaggc tggaagctct gatgcgccga
    28801 accaacggcc aggccagcaa cgagctgcgc cacggcaacg tcatgctaga ccccggcaaa
    28861 cgtatcgcca cgctggctgg cgacccctta acgctgaaac caaaagaatt tgccctgctg
    28921 gaattactga tgcgtaacgc tggtcgggta ctgccgcgca aactgattga agagaaactg
    28981 tatacctggg acgaagaggt caccagtaat gccgttgaag tgcatgtgca tcatctgcga
    29041 cgcaaactcg gtagtgattt tattcgtacc gtgcatggta ttggctacac attaggtgag
    29101 aaatgaaatt tacccaacgt cttagtctgc gagtcaggct gacgctaatc tttttaattc
    29161 tggcctcggt gacctggctg ctttccagct ttgtcgcctg gaaacaaaca acggataacg
    29221 tcgatgagtt gttcgacacc caactgatgc tgtttgccaa acggttaagt acgctcgatc
    29281 tcaacgaaat caacgcggcg gatcgcatgg cacagacgcc aaataaatta aaacacggtc
    29341 atgttgatga cgatgcgctg acttttgcca tctttaccca cgacggcaga atggtcctta
    29401 acgatggcga taacggagaa gatattcctt atagctatca gcgggaaggt tttgctgacg
    29461 ggcaactggt cggtgaagac gatccctggc gttttgtctg gatgacctca cctgatggca
    29521 aatatcgcat cgttgtgggt caggaatggg aataccgtga agacatggcg ctggcgattg
    29581 ttgccggaca attgatcccg tggctggtcg cactgccaat tatgttaatc atcatgatgg
    29641 tactactggg tcgtgaactc gcgccgctga acaaactggc gctggcacta cgtatgcgtg
    29701 accctgactc ggaaaaacca ctaaacgcga cgggcgtacc cagcgaagtg cgcccactgg
    29761 ttgagtcgct aaatcaactg ttcgcccgca cacatacgat gatggttcgt gaacgacgct
    29821 ttacctccga cgcagctcac gaacttcgta gcccgttaac ggcgctgaaa gtgcaaaccg
    29881 aagttgctca gctctctgac gatgatccgc aggcgcggaa aaaagcgctg cttcaattac
    29941 attccggaat cgatcgtgct actcgtctgg ttgatcaact gctcacgcta tcgcggctgg
    30001 actcactgga taaccttcag gacgtcgcgg agatcccgct tgaagatctc ctgcaatcgt
    30061 cggtgatgga tatttaccac acggcgcagc aggcgaacat tgacgtgcga ctgacactca
    30121 atgccaacgg catcaaacgc accggacaac cgctattgct aagtttgttg gtgcgaaatt
    30181 tgctggataa cgccgtgcgc tacagtccac agggcagcgt ggtggacgtc acgctgaatg
    30241 ccgataattt catcgtgagg gataacggcc ccggtgtgac accagaggca ctggcgagaa
    30301 ttggcgaacg cttctatcgc ccacccggac aaaccgctac cggcagtgga cttgggctat
    30361 caattgtcca gcgaatcgcc aagctgcatg acatgaatgt tgaatttggg aatgcggaac
    30421 aaggtggatt tgaggcgaaa gtaagctggt aacttctctg tctatgagag ccgttaaaac
    30481 gactct
//
LOCUS       DAZKGF010000038        30303 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZKGF010000038 DAZKGF010000000
VERSION     DAZKGF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.038"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..155)
                     /locus_tag="ACHOQV_001699"
     CDS             complement(<1..155)
                     /locus_tag="ACHOQV_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFP9630088.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(208..1464)
                     /gene="xapB"
                     /locus_tag="ACHOQV_001700"
     CDS             complement(208..1464)
                     /gene="xapB"
                     /locus_tag="ACHOQV_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFP9630089.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(1525..2358)
                     /gene="xapA"
                     /locus_tag="ACHOQV_001701"
     CDS             complement(1525..2358)
                     /gene="xapA"
                     /locus_tag="ACHOQV_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFP9630090.1"
                     /translation="MSQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            2606..3370
                     /gene="yfeN"
                     /locus_tag="ACHOQV_001702"
     CDS             2606..3370
                     /gene="yfeN"
                     /locus_tag="ACHOQV_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFP9630091.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(3408..4334)
                     /gene="yfeR"
                     /locus_tag="ACHOQV_001703"
     CDS             complement(3408..4334)
                     /gene="yfeR"
                     /locus_tag="ACHOQV_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9630092.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFILHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWEVVRGQGRALMAGREGDPLYQI"
     gene            4424..5422
                     /gene="yfeH"
                     /locus_tag="ACHOQV_001704"
     CDS             4424..5422
                     /gene="yfeH"
                     /locus_tag="ACHOQV_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFP9630093.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(5419..5637)
                     /gene="ypeB"
                     /locus_tag="ACHOQV_001705"
     CDS             complement(5419..5637)
                     /gene="ypeB"
                     /locus_tag="ACHOQV_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFP9630094.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(5639..7654)
                     /gene="ligA"
                     /locus_tag="ACHOQV_001706"
     CDS             complement(5639..7654)
                     /gene="ligA"
                     /locus_tag="ACHOQV_001706"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP9630095.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHEVEKD
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAVKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPVVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(7725..8723)
                     /gene="zipA"
                     /locus_tag="ACHOQV_001707"
     CDS             complement(7725..8723)
                     /gene="zipA"
                     /locus_tag="ACHOQV_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFP9630096.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQLVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            8953..9714
                     /gene="cysZ"
                     /locus_tag="ACHOQV_001708"
     CDS             8953..9714
                     /gene="cysZ"
                     /locus_tag="ACHOQV_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFP9630097.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            9899..10870
                     /gene="cysK"
                     /locus_tag="ACHOQV_001709"
     CDS             9899..10870
                     /gene="cysK"
                     /locus_tag="ACHOQV_001709"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP9630098.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            11254..11511
                     /gene="ptsH"
                     /locus_tag="ACHOQV_001710"
     CDS             11254..11511
                     /gene="ptsH"
                     /locus_tag="ACHOQV_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFP9630099.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            11556..13283
                     /gene="ptsI"
                     /locus_tag="ACHOQV_001711"
     CDS             11556..13283
                     /gene="ptsI"
                     /locus_tag="ACHOQV_001711"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFP9630100.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     TDLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            13324..13833
                     /gene="crr"
                     /locus_tag="ACHOQV_001712"
     CDS             13324..13833
                     /gene="crr"
                     /locus_tag="ACHOQV_001712"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9630101.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(13875..14726)
                     /gene="pdxK"
                     /locus_tag="ACHOQV_001713"
                     /gene_synonym="thiJ"
     CDS             complement(13875..14726)
                     /gene="pdxK"
                     /locus_tag="ACHOQV_001713"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFP9630102.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            14831..15199
                     /gene="yfeK"
                     /locus_tag="ACHOQV_001714"
     CDS             14831..15199
                     /gene="yfeK"
                     /locus_tag="ACHOQV_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFP9630103.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDDHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(15202..16113)
                     /gene="cysM"
                     /locus_tag="ACHOQV_001715"
     CDS             complement(15202..16113)
                     /gene="cysM"
                     /locus_tag="ACHOQV_001715"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFP9630104.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MLAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPFAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(16247..17344)
                     /gene="cysA"
                     /locus_tag="ACHOQV_001716"
     CDS             complement(16247..17344)
                     /gene="cysA"
                     /locus_tag="ACHOQV_001716"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFP9630105.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(17334..18209)
                     /gene="cysW"
                     /locus_tag="ACHOQV_001717"
     CDS             complement(17334..18209)
                     /gene="cysW"
                     /locus_tag="ACHOQV_001717"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFP9630106.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(18209..19042)
                     /gene="cysT"
                     /locus_tag="ACHOQV_001718"
     CDS             complement(18209..19042)
                     /gene="cysT"
                     /locus_tag="ACHOQV_001718"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFP9630107.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(19042..20058)
                     /gene="cysP"
                     /locus_tag="ACHOQV_001719"
     CDS             complement(19042..20058)
                     /gene="cysP"
                     /locus_tag="ACHOQV_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFP9630108.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(20216..21007)
                     /gene="ucpA"
                     /locus_tag="ACHOQV_001720"
     CDS             complement(20216..21007)
                     /gene="ucpA"
                     /locus_tag="ACHOQV_001720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFP9630109.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(21136..21993)
                     /gene="murR"
                     /locus_tag="ACHOQV_001721"
     CDS             complement(21136..21993)
                     /gene="murR"
                     /locus_tag="ACHOQV_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFP9630110.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKEQALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSELVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIER
                     SSELTQRLK"
     gene            22157..23053
                     /gene="murQ"
                     /locus_tag="ACHOQV_001722"
     CDS             22157..23053
                     /gene="murQ"
                     /locus_tag="ACHOQV_001722"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9630111.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSQEGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGPEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23057..24481
                     /gene="murP"
                     /locus_tag="ACHOQV_001723"
     CDS             23057..24481
                     /gene="murP"
                     /locus_tag="ACHOQV_001723"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFP9630112.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            24486..25787
                     /gene="pbp4b"
                     /locus_tag="ACHOQV_001724"
     CDS             24486..25787
                     /gene="pbp4b"
                     /locus_tag="ACHOQV_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFP9630113.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSALMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDAIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPCSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSDTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(25845..26744)
                     /gene="yfeX"
                     /locus_tag="ACHOQV_001725"
     CDS             complement(25845..26744)
                     /gene="yfeX"
                     /locus_tag="ACHOQV_001725"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFP9630114.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSIHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(26840..27415)
                     /gene="yfeY"
                     /locus_tag="ACHOQV_001726"
     CDS             complement(26840..27415)
                     /gene="yfeY"
                     /locus_tag="ACHOQV_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFP9630115.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(27476..27925)
                     /gene="yfeZ"
                     /locus_tag="ACHOQV_001727"
     CDS             complement(27476..27925)
                     /gene="yfeZ"
                     /locus_tag="ACHOQV_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 family protein"
                     /protein_id="HFP9630116.1"
                     /translation="MKSTEFHPVHYDAYGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(27912..28337)
                     /gene="ypeA"
                     /locus_tag="ACHOQV_001728"
     CDS             complement(27912..28337)
                     /gene="ypeA"
                     /locus_tag="ACHOQV_001728"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFP9630117.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            28551..29420
                     /gene="amiA"
                     /locus_tag="ACHOQV_001729"
     CDS             28551..29420
                     /gene="amiA"
                     /locus_tag="ACHOQV_001729"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFP9630118.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            29424..>30303
                     /gene="hemF"
                     /locus_tag="ACHOQV_001730"
     CDS             29424..>30303
                     /gene="hemF"
                     /locus_tag="ACHOQV_001730"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFP9630119.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGTEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEDDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKTMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPEDGSPEAALSEFI"
BASE COUNT         7399 a         8170 c         7521 g         7213 t
ORIGIN      
        1 ggcataaagt atttcaccac cagatatccg acatacataa acacaatgga taatataccc
       61 ggtaatacat ttggtacagg aataggcaag cgcacgattg taaacaaagc accaatgcca
      121 aaaccaacgc cagaagaaaa taaaatatct ttcactaatt aacctttatc ataaaagcag
      181 ccccaaggaa cagagctgcg aatcctttta atgtgtaact gaacgatgcg ttatcttttc
      241 agggtcatgg ttatatttaa acccaaagaa aaatatcact gcgaggaaaa gagcataccc
      301 tgcaaacatc agccaaatgg tatgccaatc ttttacgcca tccaccgaga agtaatccac
      361 tgccataccg ctcagaatcg agccaaccca tgcgccgaca ccatttacca tggtcataaa
      421 aaggccctgg gcgctggcac gaatgctgga atcaacttcc tgttcgacaa ataccgaacc
      481 agaaatattg aagaaatcga atgcacagcc ataaacaatc atcgacagca gcagcaaaat
      541 aaacccggtt ggtgatgggt cgccataggc aaagaagcca aagcgcagcg tccacgccac
      601 catactcatc agcatgacgg ttttaatacc gaaacgcttc aaaaagaacg gtatcgtcag
      661 aatgaaaccg acttcagcca tctgtgaaac tgacagtaaa atggacggat atttcaccac
      721 gaagctatca gcaaactcag gattacgggc gaaatcatgc aggaacgggt tgccaaaaac
      781 gttggtaatt tgcagcaccg cgcccagcat catggcgaag aggaaaaaga tagccatgcg
      841 tggattttta aacagcacga aggcatccag acccagcttg ctggcaagcg atgtagtcgc
      901 ttttttctcc gcaaccggaa tcttcggcaa agtcagcgca taagccgaca gcagcaatga
      961 cgcaccggac gcgatataca gctgcagact actcaattcc agatgcagca ggcttactgc
     1021 ccacatggcg acaatgaacc ccaccgtacc aaaaacgcga atgggcggga aagcggtcac
     1081 cgggtcaagc cctgcctggg caagacagga ataagagacg ctgttcgata acgcaatagt
     1141 cggcataaac gccatcgcat tgactaacat cacccaaaac atcatatccg gatcagttac
     1201 ggatgccgca taaaaaagta cgcccgcaca caccaggtga cacagcatgt atgcacgttc
     1261 tgcacgcagc catttgtctg cgatgatccc cattataccc ggcataataa tcgcggcgat
     1321 ccctttggaa ctgtaaacca tgccaacatt agcgccggtg aaatgaaggg tattaatcat
     1381 gtaagaaccg agggtaacca gccagctccc ccagataaaa tattgcaaaa aggacattac
     1441 ctttaagcgc aaagcgatgc tcataatcgt ttccttgtcg catcatatgg cctcactcag
     1501 taaggccgtt tattgttgtt ttactcaggc aattttgcgc agaaagccgc aaataagatt
     1561 aataaagttt tgctttgaga gttccgctgc tgccagcgtt tgggcatgag aaagcttcac
     1621 gtcactcaga ccttccgcca tgttggtaat cgcagagacc gcaacgactt taagttcgca
     1681 atggcgagct gaaataacct caggcaccac agacatacca acaacatccc caccaataat
     1741 ttgcatcata cgaatttccg ccgcagtctc gaaattcggc cccggatacg agacgaacac
     1801 gccctccgtc agagggaacc cctcttcttt cgccactttt tgtaacagtg cgcggtattc
     1861 cgcatcgtag gcattcgcca gcgagaagaa gcgctcacca aaacgttcat cattaagacc
     1921 caccatcggc gttcccggca tggtgttgat gtgatctttc aatgcgacca gactgccggc
     1981 ccccacttca ggacgcagtg agccagccgc attggtgcag aacagcaact cgcagcccag
     2041 caacttaaag gtacggattg catccgtcat gatggtcatc ccacgacctt cgtagaaatg
     2101 tccgcgacct ttcatgcacg ccactggcac cccctggaga taccccagca ccagctcacc
     2161 cgcatgaccg tgtacggtac ttaccgggaa cccaggcagc ttttcgtagg aaattgcgac
     2221 cgcgttctca atctgatcgg ccagcgcgcc cagcccggaa cctaaaataa aggccactcg
     2281 tggcgtgaaa tcaggtttat aagtcttgat aatatctacg caatacagtg ggttatgaga
     2341 aaactgagcc tgagacatat atatcctttt tttgtagggt ggaatctaac gcctttttat
     2401 aacgagattg ccctacctca taccaataca cttttttcat gtatccgata gttattcgat
     2461 atcgatatat ttatattttt tcactattaa taggctgaaa aaactgtatt ggtactctcc
     2521 tttcccttgc attaacaata tgccgctttt aatcaacctt ttattaagca atcaactaat
     2581 aacgatcggc atagggaatt atgctatgaa aaaacatctt tttactctga cactctcttc
     2641 tgtattggcg ataccagcag tatcccatgc agaatttaaa ggcggttttg ctgatatagg
     2701 tattcattat cttgactgga ctagcagaac cactgagaaa tcgtcgacca aatcacacaa
     2761 agatgatttt ggatatctgg aactggaagg cggcgctaac tttagttggg gagaaatgta
     2821 cgggttcttc gactgggaaa acttttataa tgatcgtcat gataaaccgg gtagcgagca
     2881 gcgttatacc tttaaaaata ccaaccgtat ttacctgggt gacaccggat ttaatctcta
     2941 cttgcatgcg tatggcacct acggttctgc gaatcgcgtg aatttccatg acgatatgtt
     3001 cctttacggt atcggctaca atttcaccgg tagcggttgg tggttcaaac cgttctttgc
     3061 aaaacgctat acagaccaaa cctattatac cggcgataac ggttatgtcg ccggttgggt
     3121 tgcaggttac agctttatgc tgggcagtga gaaattcacc ctcaccaact ggaacgagta
     3181 cgagtttgat cgtgatgcca cctacgcggc aggtaatggc ggcaaagaag gtctgaacgg
     3241 tgccgtcgca ctttggtgga atgcaacttc acacatcacc acaggaattc agtatcgtta
     3301 tgcggatgac aaactgggcg aagattttta ccaggatgca ataatctact ccatcaaatt
     3361 taatttctga ttgattactg ccggggctcc cggcagtatg aatagctcta tatctgatac
     3421 agcggatccc cttcccgccc agccattagc gcacggccct ggcctcgtac cacttcccat
     3481 aacgcttgtg cggcggtgga aagtgaacgg ttcttcctgc gcaccagcat caactggcgt
     3541 tccaccaccg gcgtaatgcg ctttaccacc agcgggctac cttcaggtaa cggcagcgcc
     3601 agtgcgggaa gaatactaat gccgatgcct gcggctacca tcgggaacag cgtcgccgga
     3661 tgaccaatct cctgcacaat attcgcctga ataccgttgc gcgccagtgc tgcatcaatc
     3721 agcggtcggc tgcctgacgc gtaatcctgc aacaccaatt tcgctccctg taatgcctgc
     3781 cagggtacat aatcttccgc ggccaaagcg ctatcgcgat ggcaaagcaa gaagaaaggc
     3841 tcggaaagaa tcgcttcaca ttgcagatcg ctcactgggc ctggatcgat gacgatgcca
     3901 aaatcgacat ctccctggcg aatactttcc atcacccact gctgcggacg atcgtgcagg
     3961 ataaactgaa tatctggata gcggcgatgg ctttcagcaa tgcattgcgg aataagatgc
     4021 gcggaaatgg tctggctggc agcgacccga acttttccgc tcagttgttg ccccatacgc
     4081 ccggtatcgc gcaacgtgct gttaagttca tccagcagtc gctcaagacg caaagccaac
     4141 tgctgccctg catctgtaag caccacttca cgcgtggttc tgtccagcag gcgaacacca
     4201 gtatgatttt ccagctcctt cacactgtga ctcactgccg actggctcag gccgatacgc
     4261 tctcctgcac gactaaaact tttctcctgc gctactgtga cgaaaacctt taattgtttt
     4321 aaagaataat tcatcttttt tcttcatgaa tggatgcaat aaatcaattt tatttctcaa
     4381 actggaagaa gcacaataga accatcgatc atctggagtc tttatgaaac tttttcgtat
     4441 cctcgatcct ttcaccttaa ccctgatcac ggtggtgttg ctggcctctt tctttccggc
     4501 cagaggcgat ttcgtcccct tctttgaaaa tctgaccact gcagctattg ccctgctgtt
     4561 ctttatgcac ggcgcgaagt tgtcgcgtga ggcgattatt gctggcggtg ggcactggcg
     4621 actgcatttg tgggtaatgt gcagcacctt cgtattgttt ccgattctgg gcgtgctgtt
     4681 tgcctggtgg aaaccggtaa atgtcgaccc gatgctctac tccggttttc tctacttgtg
     4741 cattctcccc gctaccgtgc agtctgcaat cgccttcacg tcaatggcgg gcggtaacgt
     4801 tgcggcggcg gtttgttctg cgtcagcatc cagcctgctt gggattttcc tttcaccatt
     4861 gctggttggt ctggtgatga atgttcacgg tgcgggaggc agccttgagc aggtcggtaa
     4921 aattatgctg caattgctac tgccgtttgt gttggggcat ctttcccggc cgtggattgg
     4981 tgactgggtg tcgcgcaata aaaaatggat tgcgaaaact gaccagacgt ccattctgtt
     5041 ggtggtttat acggcattca gcgaagccgt cgttaacggt atctggcaca aagttggctg
     5101 gggatcattg ctgtttatcg tggtggtcag ttgcgttctt ctggctatcg tgattgtagt
     5161 taacgtcttt atggcacgcc gactgggctt caataaggca gatgaaatta ctatcgtctt
     5221 ttgtggttcg aaaaagagtc tggcaaatgg catcccgatg gcaaacattc tgttccccac
     5281 atcggtgatc ggtatgatgg tgctgcccct gatgattttc catcagatcc aattgatggt
     5341 ctgtgcggtg ctggcgcgtc gatacaaacg ccagaccgaa cagttacagg cgcagcagga
     5401 aagcagcgcc gataaagctt aaagcggacg cttcaggggc tggaccagtt gcgtcagccc
     5461 ctcggtttta atcagcagcg tgatttgcat tagctcaccg agcttccctg ccgggaactc
     5521 atctttgcgg gcaaaccaca gcagatactc ctccggcagg tcgattaagc gacgcccttt
     5581 gtacttgcca aacggcatta tcgtattggc tatttcaatc agctgctctt tttccatctc
     5641 agctacccag caaacgcagc atttccgttt cgtcgatgac ttcaataccc agttcctgcg
     5701 ccttcgccag tttagatcct gcagcttcac ccgctatcac cagatcggtt ttcttcgaca
     5761 cgctgcccgc gactttcgcg cccagttcga ccagtcgagc tttagcgtca tcacgcgaca
     5821 tctggcttaa gctgcccgta agcaccacgg ttttaccagc aaacgggctg tcaatctctt
     5881 ccgcgttaat caccaccggc gcaggccagt gaacgccttc cgccaacagc tcgctgatga
     5941 cattgcggtt gctttcttcg gcaaagaagt tgtgaacgtg ggaggcaacg acaatgccaa
     6001 catcaggcac cttttgcagc tcttcaatcg aagcggcttc cagcgcttcc agcgtgccga
     6061 aatatgccgc cagacctgct gcggtggcct cgccgacttc acggatgcca agtgcataga
     6121 ggaagcgagc aaaggtggtt tctttcgctt tttccagcgc gttaaccacg ttttgtgccg
     6181 atttcggccc catacgctcc agtccggtca gtttgactgc ggtgagtttg aacagatccg
     6241 ccggagtgtg gacatattct ttttcaacca gttgatcgat gattttgtcg cccattccgt
     6301 caacatccat cgcacggcgg gaaacaaagt gtttcagaga ctctttacgc tgcgcaccgc
     6361 aaatcaggcc accggtacag cgggcaaccg cttcaccttc cacacgctcg acgtcagaac
     6421 cacataccgg acaatgcgtc gggaatacaa cctcacgggt atcttccggg cgttcagaaa
     6481 gcacgacgtt aaccacctgc gggatcacgt cgccagcgcg gcgaataacc actttatcgc
     6541 caatgcgtaa accaagacgt tcgatttcgt cagcattatg cagtgtagcg ttactcacca
     6601 acacgccagc aacatggaca ggttccagac gcgcaacagg cgtaatcgcg ccagtacgcc
     6661 caacctgaaa ctcgacgtca cgcacaaagg tcatctgttc ctgcgccggg aatttaaacg
     6721 ctaccgccca gcgcggggca cgcgcgacaa agccaagctg ctcctgctgt gccagtgagt
     6781 tgaccttaat caccacgccg tcgatatcaa agcccagcgt cggacggtct ttttctactt
     6841 cgtgataaaa cgccagcact tcttccgccg attcacaaag tgttacccga tcactgacag
     6901 gtaatcccca cgctttaaat tgcattaagc gtcccaaatg ggtatccggc aattcaccgc
     6961 cttccagaac accaacgcca tagcagaaga aagtgagcgg ccgcttcgct gtaatacgcg
     7021 gatcaagctg acgcagtgaa ccagctgccg cattacgtgg gttagcaaac actttcccgc
     7081 ccgtgcgtcg cgcatcttcg ttaatctttt cgaaccccgc ctgcggcagg aacacttcac
     7141 cacgcacttc cagacgcgcc gggatattct ctccgtgtag cttcagcgga atggcgcgaa
     7201 tagtacgcac attagacgtg atatcttccc cggtggtgcc atcgccacgg gtggcggcac
     7261 tgactaaaac accattttca tacagaatac tgacggcaag accatccagc ttcagctcac
     7321 agcaccaggt gactttctcg ttgcttttca gacggtcctg cacacgtttg ttgaaagcaa
     7381 gaaagctttc ttcatcaaaa acgttatcca gcgacagcat tggcacttca tggcggatct
     7441 ggctgaaagc cgccagcggc gcagcaccta cacgttgggt aggcgaatca ggcgtaatca
     7501 gttctggatg tttggtttcc agctcgcgca attcgcgcat cagcctgtcg tattcagcgt
     7561 cgggaatttc cggcgcatcc atcacatgat aaagatattc atgatggcga agcgtcgttc
     7621 gcagttctgt cagttgttgt tcgattgatt ccatatcgca ccatcaatgc taaaaacccc
     7681 cgacaagcgg gggttcgaag aggagttaat ttgccttaag tgtatcaggc gttggcgtct
     7741 ttgacttcgc ggatgatgtc ctggtactcg cgcaatttct gcggagtcat catacggcgc
     7801 tgatcgtcaa gaacaacacc gcccacttca tcggcaatat gctgcgcaga ttgcagcatc
     7861 agcttgaagt tctgcagctc gtcaccgtaa gacggtacct gcataaagat agtgacaccc
     7921 ggagtagtga aatccttcat ttcaggatca aaggttcccg gtttcaccat attcgccagg
     7981 ctgaataacg ccgggccgct gccatccggg ctaagatgac gatggtaaat attcatatcg
     8041 ccaaaaatga agcccgcttg ttgaatgctg ttaagaagca gttcaccgtt tagctcgcta
     8101 ccgtgatgcg ccgcgacgtt catgataatc accgcttctt tgcgcttcgg tttatccata
     8161 actggagccg gttccgctac aggctcaggc tgtggtgccg ctacgggttc tgcaggctgg
     8221 aaagcctgtt gtgccggttg cggtgctgaa tgcactggct gcggcgctgg cgcgacctgt
     8281 ggcgaaactg gctgctgcaa cggctgttca ggctgcggct gataggcagg ctgctgcacg
     8341 ggttgctgca ccagctgcgc tggacgcgga gcatgttgcg gcggtacctg cgcttcaggc
     8401 ggctgctgga ccggttgacg cggctgcgca gacgcataag gcggttggta ctggtgttgc
     8461 ggcgacggac gagcagcctc atgctcctgg gcgttagccg gggcatgatt cacgcggtga
     8521 actcgaacct caccaacgcc ctcatcatct tcgacatcct cgtcataaga atcgtcgtca
     8581 cgttttgact tcattcgttt taatggccga tcgcggaaca tagaagatcg ttctttacgg
     8641 ctggtccaga aaccatgtac cagtaaagcg attatggcga tcgcgccaac aatgattaat
     8701 atcagacgca aatcctgcat cattatattc tctgttgttc taacaccttg ccaccacggc
     8761 aaacatttac tcactaagag tatttgtcga ttacctcaag tgcaagtgca ctattaactt
     8821 tcacagcaca aagatagatg aaatcgtgct ttttgctgtt ttttcgaaca tatcctaact
     8881 gtccattgcg caattacccg gtaaaatacg cagaattttc ctgggattgg tcaaaaggag
     8941 ctcatcctga ttatggtttc atcattcaca tctgccccac gcagcggttt ttactatttt
     9001 gcgcaaggct ggaagctcgt ctcgcaacct gggattcggc gtttcgttat tttaccgctg
     9061 ctggtcaata ttttgttgat ggggggcgca ttctggtggc tctttacgca actcgatgtc
     9121 tggatcccga ctctcatgag ttatgttccg gactggttac aatggctgag ctatctgttg
     9181 tggcccctgg ctgtcatctc tgtgctgtta gtatttggtt atttcttttc aacgattgct
     9241 aactggattg ccgctccgtt caacggttta ttggctgaac aactggaagc aagactgact
     9301 ggcgctacac cgccagatac cggaattttc ggtatcatga aagatgtgcc gcgaatcatg
     9361 aaacgcgaat ggcaaaaatt tgcctggtat ctgccgcgcg caattgtatt gctaattctt
     9421 tacttcatcc ctggtattgg gcaaaccgtc gcgccagtac tgtggttcct gtttagcgcc
     9481 tggatgttag ccatccagta ttgcgattac cccttcgata accacaaagt gccgtttaaa
     9541 gagatgcgca ccgccctgcg cacacgcaaa atcaccaata tgcagtttgg tgctttaacc
     9601 agcctgttta cgatgatccc gttgcttaat ctgttcatca tgcccgttgc cgtttgtggc
     9661 gcgacggcga tgtgggtcga ttgctatcgc gataaacacg cgatgtggcg gtaacaatct
     9721 accggttatt ttgtaaaccg tttgtgtgaa acaggggtgg cttatgccgc cccttattcc
     9781 atcttgcatg tcattatttc ccttctgcat atagatatgc taaatcctta cttccgcata
     9841 ttctctgagc gggtatgcta cctgttgtat cccaatttca tacagttaag gacaggccat
     9901 gagtaagatt tttgaagata actcgctgac tatcggtcac acgccgctgg ttcgcctgaa
     9961 tcgcatcggt aacggacgca ttctggcgaa ggtggaatct cgtaacccca gcttcagcgt
    10021 taagtgccgt atcggtgcca acatgatttg ggatgccgaa aagcgcggcg tgctgaaacc
    10081 aggcgttgaa ctggttgaac caaccagcgg taataccggg attgcactgg cctatgtcgc
    10141 tgccgctcgc ggttacaaac tcaccctgac tatgccagaa accatgagta ttgaacgccg
    10201 caagctgctg aaagcgttag gtgcaaacct ggtgctgacc gaaggtgcta aaggcatgaa
    10261 aggcgcaatc caaaaagcag aagaaattgt cgccagcaat ccagagaaat acctgctgct
    10321 gcaacaattc agcaatccgg caaaccctga aattcacgaa aaaaccaccg gtccggagat
    10381 ctgggaagat accgacggtc aggttgatgt atttattgct ggcgttggga ctggcggtac
    10441 gctgactggc gtcagccgct acattaaagg caccaaaggc aagaccgatc ttatctctgt
    10501 cgccgttgag ccaaccgatt ctccagttat cgcccaggcg ctggcaggtg aagagattaa
    10561 acctggtccg cataaaattc agggtattgg cgctggtttt atcccggcta accttgatct
    10621 caagctggtc gacaaagtca ttggcatcac caatgaagaa gccatttcta ccgcacgtcg
    10681 tctgatggaa gaagaaggca ttcttgctgg tatctcttct ggagcagctg ttgctgcggc
    10741 gttgaaacta caagaagatg aaagctttac caataagaat attgtggtta ttctgccgtc
    10801 atcgggtgag cgttatttaa gcactgcatt gtttgccgat cttttcactg agaaagaact
    10861 gcaacagtaa tgccagcttg taaaaaatgc gtaaaaaagc acctttttag gtgctttttt
    10921 gtggcctgct tcaaactttc gcccctcctg gcattgattc agcctgtcgg aactggtatt
    10981 taaccagact aattattttg atgcgcgaaa ttaatcgtta caggaaaagc caaagctgaa
    11041 tcgattttat gatttggttc aattcttcct ttagcggcat aatgtttaat gacgtacgaa
    11101 acgtcagcgg tcaacacccg ccagcaatgg actgtattac gctcttcgtg cgtcgcgtct
    11161 gttaaaaact ggcgctaaca atacaggcta aagtcgaacc gccaggctag actttagttc
    11221 cacaacacta aacctataag ttggggaaat acaatgttcc agcaagaagt taccattacc
    11281 gctccgaacg gtctgcacac ccgccctgct gcccagtttg taaaagaagc taagggcttc
    11341 acttctgaaa ttactgtgac ttccaacggc aaaagcgcca gcgcgaaaag cctgtttaaa
    11401 ctgcagactc tgggcctgac tcaaggtacc gttgtgacta tctccgcaga aggcgaagac
    11461 gagcagaaag cggttgaaca tctggttaaa ctgatggcgg aactcgagta atttcccggg
    11521 ttcttttaaa aatcagtcac aagtaaggta gggttatgat ttcaggcatt ttagcatccc
    11581 cgggtatcgc tttcggtaaa gctctgcttc tgaaagaaga cgaaattgtc attgaccgga
    11641 aaaaaatttc tgccgaccag gttgatcagg aagttgaacg ttttctgagc ggtcgtgcca
    11701 aggcatcagc ccagctggaa acgatcaaaa cgaaagctgg tgaaacgttc ggtgaagaaa
    11761 aagaagccat ctttgaaggg catattatgc tgctcgaaga tgaggagctg gagcaggaaa
    11821 tcatagccct gattaaagat aagcacatga cagctgacgc agctgctcat gaagttatcg
    11881 aaggtcaggc ttctgccctg gaagagctgg atgatgaata cctgaaagaa cgtgcggctg
    11941 acgtacgtga tatcggtaag cgcctgctgc gcaacatcct gggcctgaag attatcgacc
    12001 tgagcgccat tcaggatgaa gtcattctgg ttgccactga tctgacgccg tccgaaaccg
    12061 cacagctgaa cctgaagaag gtgctgggtt tcatcaccga cgcgggtggc cgtacttccc
    12121 acacctctat catggcgcgt tctctggaac tgcctgctat cgtgggtacc ggtagcgtca
    12181 cctctcaggt gaaaaatgac gactatctga ttctggatgc cgtaaataat caggtttacg
    12241 tcaatccaac caacgaagtt attgataaaa tgcgcgctgt tcaggagcaa gtggcttctg
    12301 aaaaagcaga gcttgctaaa ctgaaagatc tgccagctat tacgctggac ggtcaccagg
    12361 tagaagtgtg cgctaacatt ggtacggttc gtgacgttga aggtgcagag cgtaacggcg
    12421 ctgaaggcgt tggtctgtat cgtactgagt tcctgttcat ggaccgcgac gcgctgccca
    12481 ctgaagaaga acagtttgct gcttacaaag cagtggctga agcgtgtggc tcgcaggcgg
    12541 ttatcgttcg taccatggac atcggcggcg acaaagagct gccatacatg aacttcccga
    12601 aagaagagaa cccgttcctc ggctggcgcg ctatccgtat cgcgatggac cgtaaagaga
    12661 tcctgcgcga tcagctccgc gctatcctgc gtgcctcggc tttcggtaaa ttgcgcatta
    12721 tgttcccgat gatcatctct gttgaagaag tacgtgcact gcgcaaagag atcgaaatct
    12781 acaaacagga actgcgcgac gaaggtaaag cgtttgacga gtcaattgaa atcggcgtaa
    12841 tggtggaaac accggctgcc gcaacaattg cacgtcattt agccaaagaa gttgatttct
    12901 ttagtatcgg caccaatgat ttaacgcagt acactctggc agttgaccgt ggtaatgata
    12961 tgatttcaca cctttaccag ccaatgtcac cgtccgtgct gaacttgatc aagcaagtta
    13021 ttgatgcttc tcatgctgaa ggcaaatgga ctggcatgtg tggtgagctt gcaggcgatg
    13081 aacgtgctac acttctgttg ctggggatgg gtctggacga attctctatg agcgccattt
    13141 ctatcccgcg cattaagaag attatccgta acacgaactt cgaagatgcg aaggtgttag
    13201 cagagcaggc tcttgctcaa ccgacaacgg acgagttaat gacgctggtt aacaagttca
    13261 ttgaagaaaa aacaatctgc taatccacga gatgcggccc aatttactgc ttaggagaag
    13321 atcatgggtt tgttcgataa actgaaatct ctggtttccg acgacaagaa ggataccgga
    13381 actattgaga tcattgctcc gctctctggc gagatcgtca atatcgaaga cgtgccggat
    13441 gtcgtttttg cggaaaaaat cgttggtgat ggcattgcta tcaaaccaac tggaaacaaa
    13501 atggtcgcgc ctgtagacgg caccattggt aaaatctttg aaaccaacca cgcgttctct
    13561 atcgaatctg atagcggcgt tgaactgttc gtccacttcg gtatcgacac cgttgaactg
    13621 aaaggcgaag gcttcaagcg tattgctgaa gaaggtcagc gcgtgaaagt tggcgatact
    13681 gtcattgaat ttgatctgcc gctgctggaa gagaaagcca agtctaccct gactccggtt
    13741 gttatctcca acatggacga aatcaaagaa ctgatcaaac tgtccggtag cgtgactgtg
    13801 ggtgaaaccc cggttatccg catcaagaag taattcttgc cgcagtgaaa aatggcgccc
    13861 atcggcgcca ttttttatgc ttccgccagc ggcggcaaaa tcaattcatc gctctcatgc
    13921 tgctgggtgt agcgcatcac ttccagtacg cgcaaccccg cacggtgcac cgcatcgttt
    13981 aacgccttcc ctttcagcaa tccactgata agctgagcac aaaacaggtc gccagtccct
    14041 ttcaggtcgg tttttacccg tgaatgggaa atgacattca cgctgtcggc actgaccacc
    14101 accacctgca tctcctgatt ttcttcatta ccggaggcgc tggtaatcac cacccatttt
    14161 aatgtgtctg aaagcagact ttttgcggca gcaatggcac tgtcgagatc gcggcaattt
    14221 ttaccggtca ggatttccag ctcaaagata ttgggggtaa ttccctgcgc cagcggcagt
    14281 aaatattgtc gatacgcctc gggaaggtca ggtttgacat aaattccgct atcaatatcg
    14341 ccaatcaccg gatcgaccat gatcaatagg tcaggatggt ctttgcgtag cgcagtcagc
    14401 cactcggcaa ggattttgat ttgcgatgcc gttcccatat agcccgtggt tacagctcga
    14461 agttggcgca gcgcatcacg ctcctgaagc gcacgcaaat agccgctaaa ccattcatcc
    14521 ggaatcgcac caccgtagaa agtgtcataa tgcggtgtat tgctcagcaa taccgtcggc
    14581 acggcaaaga cattcaggcc gttctgtttg atagcaggca ccgcaatgct gttgcctacg
    14641 ctgccgtaca ctacctgcga ctgcacggcg acgatatccg cctgcaatgc cctgctctta
    14701 tcgttaaaca acaacaaact actcatttaa ttttttctcc ttgccgatga tcctcatcgt
    14761 attccaaccg aaactttacc tgattctggc agtcaaatcg gctatcacaa aacaaggata
    14821 aggtaattca atgaagaaaa tcatttgtct ggtcattacg ctgctaatga cactccccgt
    14881 ttacgcgaag ttaactgccc atgaagaagc ccgtatcaac gccatgctgg agggattagc
    14941 acagaaaaag gatttgatat ttgtgcgtaa cggcgatgat catacctgcg atgaagcggt
    15001 ttcccacctg cgtctgaagc tcggcaatac ccgtaaccgc attgacactg ccgagcagtt
    15061 tattgataag gtagcctcgt cgtcatcgat taccgggaag ccgtatatcg tgaagatgcc
    15121 cggtaaaagc gatgagaacg cacaaccttt tttacatgcg ctgattgcgc agacggataa
    15181 aacggtgcct gcgcaataat cttaaatccc cgccccctgg ctaaaatgct cttccccaaa
    15241 caccccggta gaaaggtagc gatcgccgcg atcgcagatg atcgccacaa ccaccgcgcc
    15301 ggggttagct tttgccacgc gcagtgctcc ggcaaccgcg ccgccggagc tgacgccaca
    15361 gaatattcct tcccgcactg ccagttcgcg catggtgttt tccgcatcgc gctgatgaat
    15421 atccagcacc tcatccacca gcgaagcgtt gaaaatcccc ggcagatatt ccgcaggcca
    15481 gcggcgaatg ccgggaatgc tactgccctc ttccggctgc aggccaacaa tggtcaccgg
    15541 tttggattgt tcgcgcataa agcgtgaaac gccggtgata gtgccggttg ttcccatgct
    15601 ggagacaaaa tgggtaatgc gcccgccggt ttgctgccag atttccggcc cggtggtggt
    15661 gtaatgggcg aagggattat caggattatt gaactgatcg agcagctttc cttcaccacg
    15721 attggccatc tccagcgcca gatcgcgcgc gccttccatg ccctgctctt tggtgacaag
    15781 aatcagttcc gcaccgtatg ccagcatcgc tgcacggcgc tcctggctca tgttgtcggg
    15841 catcagcaat ttcatgcgat agcctttaag cgcggcaatc attgccagcg caatgccggt
    15901 gttaccactg gtggcttcaa tcaggacatc gcccggtttg atttctccgc gcttttccgc
    15961 ctcgacgatc atcgaaagcg ctgcacgatc tttcaccgaa cctgccgggt tattgccttc
    16021 cagtttcaac cagacttcac tgccgttatc cggcgccatt cgctgcaact tcaccagagg
    16081 cgtattgcca attgtttgtt ctaatgtact cacgatctct gtccatacgt ggtgttgcct
    16141 gatgcgacgc ttgcgcgtct tatcaggcct acaggttaca aaccttgcca ttaaaaagcc
    16201 cggatcgcgg gaagcgcctc cgggcgttta acattcactc aacctatcag gcgctttgtg
    16261 cgagagcaag ttcctcatcg cgggtttcaa tacgctcgtc gccgttatac agccgcgcat
    16321 gttgcagacc aacgaataaa cgctcgccac gctgcggggc atcgtcgcca tgcatcacaa
    16381 ccgtcagcgg ttcgttgtac caccccagcg gctgcacgac taattgggtg tagtggcctt
    16441 tcgggctggc ttccagtacc tgtaccggca gcggcgaatc gaggctggta cggcggctga
    16501 tatccacttc ccacggacgc aggaagagat ccaccggccc ctgatacgca ggtgtatagc
    16561 ccagcggcca gcgatgtgcg ccaacatgga actgcccgcc gcgaatggtt ccctgcaggc
    16621 ggttcacttc gcccataaat tcgagtacaa aacgggtcgc tggttcgcgc catacctggt
    16681 ccggtgcatc agcctgctcg atattgccct ggctcatcac cactacacga tcggctactt
    16741 cggtcgcttc ttcctggtca tgggtcacaa acacgctggt gaatttcagc tcttcatgta
    16801 actgacgcag ccagcgacgc aactctttac gcacctgcgc atccagcgcg ccaaacggtt
    16861 catcaagcag cagaatttgc ggttctacag ccagcgcgcg cgccagtgcc acacgctgtt
    16921 tctgaccgcc ggaaagctgt gctggataac gatccgccag atgcgcaagc tgcaccatct
    16981 ccagcaattt tgtcactttc gctttgatgg ctgcggcatt cgggcgctcg cgacgcggca
    17041 gcaccgtcag gccgaaagcg atattgtcga acaccgtcat atggcggaac agcgcatagt
    17101 gctggaacac gaaaccgact ttacgatcgc gcgcatgcag gcggctgacg tcggtgccgt
    17161 ggaagcgaat atgcccgctg gtttgatgct ccagcccggc gataatgcgc agcagcgtgg
    17221 ttttcccgga accggacggc cccagcaacg cgaccatctg accagaagga atatccagtg
    17281 agatatcgtt cagcacctgg gtgcgaccaa aagacttctt aatattggca atctcaatgc
    17341 tcatgatgtt cctcctgctg cgcgcgtttt tcctgattct ccagacgcca ctgcaacata
    17401 ctctttaaaa acagggtgat aatcgccatc agcgtcaaca acgcggcagc ggtaaaggag
    17461 ccgacggtgt tgtagtcttg ctccagcaat tcaatctgta acggcagcga aagtgtttcg
    17521 ccgcgaatcg agccggaaac caccgacacc gcaccaaact cgccaatcgc gcgggcgttg
    17581 gtcagcacca cgccataaag cagcgcccag cggatgttcg gcagcgtgac gcggcgaaac
    17641 atctgccagc cagacgcgcc aagcaaaatc gccgcttcgt cttcctggct gccctggctt
    17701 aacatcaccg gcaccagctc acgcaccaca aacggacacg tcacgaagat ggtgaccagc
    17761 accattcccg gccaggagaa cataatttgc agattatgtt catccagcca gccgcccagc
    17821 gggccgttag agccgtagaa cagcagatac accagaccag caaccaccgg cgacacggca
    17881 aacggaatgt ccaacagcgt cagcaatagc tggcgtccgg ggaagttaaa gcgcgtcacc
    17941 agccaggcca gcagaatacc gaacaccagg tttaccggta cggcaatcag cgcgatcatc
    18001 accgtcagcc agatggcgtg cagcatgtcc ggatcggcca gattctgtaa aaccggcatc
    18061 agccctttgc tgaacgcctg tacgaagata taaatcatcg gcaccagcag gatgaacgcc
    18121 gataccagca tcccgatgcc aatcagaaac catttgcccc agttaatcgg gcgcgcgtca
    18181 taacgcttca actgtgtcac ttccgccatt aatgacctac cacacgccga ccaaagcgac
    18241 tttgcagagt gttaattgag aacagcagca gcagcgatgc cgcgaggatc accgaggcaa
    18301 tcgcgctcgc cgccgggtaa tcaaactcct gtaagcgaac aaagatcatc agcgacgtca
    18361 cttctgtttt ccaggcgata ttcccggcga taaaaatcac cgcgccaaac tcaccgaggc
    18421 tgcgggtaaa cgacagcgcc acacccgcca ccagcgccgg agatagttcc ggcagcacca
    18481 ctttgcgaaa actctgccag cgtgttgcgc cgagcgtttc tgccgcctct tcatattccg
    18541 ggcctaactc ttccagcacc ggctgcacgg tgcgcaccac aaacggaatg ctggtaaagg
    18601 ccatcgccac cgcaatcccc agccaggtat aggtgacttt gatatcaaac ttcgccagcc
    18661 attcaccgta aaaaccgttc acggaaaaga gcgaggccag cgttaaaccg gcgactgccg
    18721 ttggcagcgc aaagggtaaa tccatcagcg catcaagcag cgtgcgaccc ggaaagcgat
    18781 agcgggttaa gatccacgcc atcagcagac cgaaaacgcc gttaaaaatc gatgccacaa
    18841 acgccgacag cagcgtcact ttataggctg cgaccacctg cgggttggtg atcacctccc
    18901 agtactgcgc ccagctcatc tgggccagtt gcatcaccag cgcggagagc ggcagcagca
    18961 aaatcaggca cacaaacagc agactggtgc cgaggcttaa ggtaaagccc ggcagcacgc
    19021 gtcttgaaga aactgcaaac attacttacg ccccgccgct aacagcttgt ctaactcgcc
    19081 gccgctggtg aagtgggttt tcatcacttc cggccaggag ccaaatttat cttccacgcg
    19141 gaacagctcg gtctgcggga atttatcttt cagtttgccc atgacttccg ggttatttac
    19201 gcggtagtaa tagtcggtga tgatggtttg cgcctgcggg ctgtagagcc agttcaggta
    19261 ggcttttgcc gctttttccg tgccgttggc ctgtacgttt ttatccaccc acgccaccgg
    19321 gaattccgcc agaatgttgg ttttcggaat caccacttca aagccctgcg cttcatactg
    19381 tttacggatg ttgttcactt ccgactcgaa gctgatcagc acatcgccca ggccgcgctc
    19441 ggcaaaagtg gtggtcgcgc cacgaccgcc agtatcgaac acttcaacgt ttttcaggaa
    19501 ctgggtcata aactgttcgg ttttggcttt atcaccaccg tcggctttgt ccgctgcgcc
    19561 ccatgccgca aggtaggtat aacgcgcgtt acccgacgtt ttcgggttcg ggaaaatcag
    19621 cttcacgtca gagcgtacca ggtcgttcca gtcgtggata ttctttgggt tgcccttacg
    19681 caccaggaag cccatggtgg agtagaacgg cgagctatta ttcggcaggc gcgactgcca
    19741 gtccgctggg atcagcttgc ctttatcgtg caggatttgt acgtcggtca cctggttata
    19801 agtgacaacg tcggctttta aaccctgcaa aattgccagc gcctgttttg atgatccggc
    19861 atgagattgt ttaatcgtca gtttgtcgcc accgttatct tttgcccatt gttgctcaaa
    19921 cggcgggttc agggcggcaa acagctcgcg ggagacgtca taagaactgt tcagcagttc
    19981 cgttgcctgt acatggcccg ccagtagcag agaagcaacc agcacgagtg agttcttttt
    20041 cagtaagtta acggccattg cgcaccctta taaatttaat gactttctaa tagccatcat
    20101 atttataacg gacgtgaaag gagtaacggt tttatatacc gtttggtgat ttggaagttg
    20161 aaaagggaat aagacgaaat cctccccaca aatgcaggga ggaaacagag gtgaatcaga
    20221 tgccgacgct aaccgtctcc ggcagtgtgc tgccgccatc aatcacattc tgtgtaccgg
    20281 ttaaatagct ggattcatcc gatgcgagga aggccgccag ttcgccgact tccagcggat
    20341 cggcgaggcg acgcatcggg attgctttcg ccatttcagt cagcaccgat tctggatctt
    20401 ccgggttcga ctggcgggca atgctttccg ccattggcgt gcgaacgtat ccagggcaga
    20461 tggcgttaac gcgaatacca gactgcgcgt actccaccgc cagcgattta gtcaggccaa
    20521 caatcgccgc tttcgttaag gcgtacgccg tttcgcccgg gtctgccacc atatcgccag
    20581 tgactgaaga catcatcaca atccggccat ctttgcgggc aatcatctcc ggcagcaccg
    20641 ccttcgtgac gttccatacg ccttttatat tgatatcaat atggaaatcg cgatcttcat
    20701 cgctcatatc aaggaaactg cccagacgac aaacgcctgc gttattcacc agaatatcaa
    20761 tgcgcccttc tttttcctta gcgcgcttaa tagctgcggc gaccgacgcc gggtcacgca
    20821 catcggcgac aaccgccgta cagcgatgac cacggccaca cagttcgtcc gccagctttt
    20881 cgatctcagg ggagatatcc agcaagatta ggtttgcgcc atgacgcgca aaagttctgg
    20941 caattccttc gccaattccc tgcaatgcgc ccgtaatcag tgctgtcttg cccgtgagtt
    21001 tacccatttt aatgctctcc ttgttaatac agagcgttac actccattcc ctctgaaaat
    21061 acaggataaa caaaatattg ctgcggatca gctcactaat tcaggaaaat ctcaatccag
    21121 tcgctgcttt atgcatcatt tcaggcgttg cgttagctca ctgctgcgct caatcatttt
    21181 caatgactcc acatcattga gttgcacaag gccaacaaac agtaaatcgg taacagaatt
    21241 ttgcgccgtg cgggtggaca tcgacgagct gcgccattcg gtttcgccag aaacggtatc
    21301 gagggtgtaa tgcgccagcc gccgcagcgg ggaatcggtt aatgaggtaa tggcaatcac
    21361 cgttgccccc tgctttcgcg ctgcctcggc gcacaagaca atctcttttt tactgccact
    21421 gtaagagata gcgatttgta catcgccttt ttttagcgcc tgagaaacgg tggcttgtac
    21481 gtgggtgtcg gcctcacagg ccacgcgata accaattttc atcagtttga acgacagatc
    21541 gcgccccacc agctccgacc cgcccaggcc cgttatctgg ataaacggtg ctttactgat
    21601 gacctcaatg attttttgca gccgcgcgta gtcaaacaac gcgcaggttt gctccagcgc
    21661 ctgctctttt tcgcgattca gtttacgggc aatcacttcc agcgaatcat cactggtaat
    21721 cgaactgtgc aggtgcagcg ccgtggcatt cgttttctcc cggctggcac tgtattcgcc
    21781 aattaacgcc atacgcaatt cagtaaatcc ttgcgcgccg agcttctggg caaacttcac
    21841 aatactcgac tggctgatgc cgagctgttt cgccatcttg cgcgaagaga cggatttcag
    21901 ctcactcacg ttggcccgca gaaagtcagc aattttttgc tcgttcccgg taaattcact
    21961 ttccgcattg cgtattttcg tcaggtacaa catcttccac tctcctgggc tggcagcaaa
    22021 aattccgctt aagttcttat gacgctcttc acactctgcg agtgtagact caatgattcc
    22081 tttaagtctg tcaattcggg atatatgatt ccatacatct gcaattattt gatcgtaaat
    22141 agtaaggtca ccaccgatgc aacttgaaaa gatgattacc gaaggctcga acgccgcttc
    22201 ggctgaaatt gaccgcgtat caacgctgga aatgtgccgg attatcaacg atgaagataa
    22261 aaccgtacca ctcgccgttg agcgcgtact gccggatatc gccgcagcga tcgatgttat
    22321 ccacgcccag gtcagcggcg gcggtcgtct gatttatctc ggtgcgggaa catcaggtcg
    22381 tctggggatt ctggatgcca gcgaatgtcc gccgacctac ggcgtgaaac cgggtctggt
    22441 ggttggatta attgctggcg gcgaatatgc cattcagcac gcggtggaag gcgcggaaga
    22501 tagccaggaa ggcggtgtga atgatctgaa aaacatcgga ttaaccgcgc aggatgtggt
    22561 ggttggtatt gccgccagtg gtcgcacgcc gtatgtgatt gccggactgg aatacgcgcg
    22621 ccagctcggc tgccgcacag tgggaatttc ctgtaatcca gggagcgcgg tttccaccac
    22681 cgctgagttt gctattacgc cgatcgttgg tcccgaggtg gttaccggtt cttcacgaat
    22741 gaaagcaggc acagcgcaga aactggtgct caatatgctc tccaccgggc tgatgattaa
    22801 atccggcaaa gtgttcggca acctaatggt cgatgtggtc gccaccaacg aaaaattgca
    22861 tgtgcgccag gtcaatatcg tcaaaaacgc taccggatgt agcgcagagc aagcggaagc
    22921 agcgttgatt gcctgcgagc gcaactgtaa aacggccatt gtgatggtgc tgaaaaatct
    22981 cgatgccgca gaagctaaaa aacgcctgga tcaacacggc ggctttattc gtcaggtttt
    23041 agacaaggaa taacccatgg ccaaagagat cagcagtgaa cttctgaaca ccattcttac
    23101 ccgggtcggt ggaccgggaa atatcgccag ttgtggtaac tgtatgacgc gcctgcgtct
    23161 gggtgtacat gacagttcac tggttgatcc caatatcaaa acgctggaag gcgtgaaggg
    23221 cgtcatttta accagcgacc aggtgcaggt cgtttttggg cctggtaaag cgcatcgtgc
    23281 cgcaaaagcg atgagcgagc tgctgggaga agcaccggta caggatgccg cagaaatcgc
    23341 cgcccagaac aaacgtcagt taaaagccaa acaaacctcc ggcgtgcaac aatttctcgc
    23401 caaattcgcc accatcttca cgccgctgat ccccggtttt attgccgctg gtctgctgct
    23461 ggggatagcg acgttaattg ccacggtgat gcacgttccg gcagatgctc agggcacgct
    23521 acccgatgcg ctgaatttta tgaaggtgtt cagcaaaggc ctgttcacct tcctggtgat
    23581 tctggtgggc tataacgccg cccaggcatt tggcggcacg ggcgtcaatg gcgcaattat
    23641 cgccgcgctc tttttgctcg gttataaccc aactgcaacc accggttact acgccggatt
    23701 tcacgatttc tttggtctgc ccatcgatcc gcgcggcaat attatcggcg tgttgattgc
    23761 cgcgtgggcc tgcgcccgca tcgaaggcat ggtgcgccgc tttatgccgg acgatctcga
    23821 catgctgttg acctcgttaa tcaccctgct aatcaccgct acgcttgcgt acctgatcat
    23881 tatgccgctg ggcggctggc tgtttgaagg catgtcgtgg ctgtttatgc acctgaacag
    23941 taatccgctc ggttgtgcag tattagccgg gctgttcctg atcgccgtgg tgtttggcgt
    24001 gcatcagggc tttattcccg tttacctcgc gttaatggac agtcagggat tcaacagctt
    24061 atttcccatc ctttcaatgg caggcgcggg ccaggtgggc gcggcgctgg cactctactg
    24121 gcgggcgcaa ccgcacagtg cgctacgcag ccaggtgcgc ggggcgatta ttcccggtct
    24181 gttgggcgtt ggcgaaccgc tgatttacgg cgtcaccctg ccccgcatga aaccgtttgt
    24241 taccgcctgt ttaggtggcg cggcgggtgg tttgtttatc gggctgatag cctggtgggg
    24301 cctgccgatg ggcttaaaca gtgcctttgg gccgtctggt ctggtggcgc tgccgctgat
    24361 gacatcagca caaggtatcc ttccggcaat ggcggtttat gcaggcggga ttctggtggc
    24421 atgggtttgc gggtttattt tcaccacgct ctttggctgc cgtaacgtca atctggactg
    24481 ataatatgaa acggacaatg ctctatcttt ctctgctggc tgtcagctgt agcgtcagcg
    24541 ccgcaaaata ccctgttctg acagaaagtt cgccagagaa agcagggttt aacgtcgaac
    24601 ggcttaatca gatggatcgc tggataagcc agcaagtgga cgccggttat cccggcgtaa
    24661 acctgctgat tataaaagat aatcagattg tttatcgcaa agcctggggc gcggcgaaaa
    24721 agtacgatgg cagcgcgtta atggcgcagc ctgtcaaagc caccaccggg acgctgtatg
    24781 acctggcttc caataccaag atgtacgcca ctaacttcgc cctgcaaaag ctgatgagcg
    24841 aaggcaaact gcatcctgac gatcggattg cgaaatatat tccagggttt gccgacagcc
    24901 cgaacgatgc gatcaaaggc aaaaacacgc tgcggatttc tgatctgctg catcacagcg
    24961 gcggtttccc ggcagatccg caatacccga ataaagcggt cgcgggcgcg ttatattccc
    25021 aggataaagg ccagacgctg gagatgatca agcgcacacc gctggagtat caaccctgca
    25081 gcaagcatat ctacagcgat gtcgattata tgctgcttgg atttatcgtc gagtcggtta
    25141 ccggtcagcc acttgaccgc tatgttgaag agtcgattta tcgcccgctc ggcctgacgc
    25201 acacggtatt taatccgctg ctgaaaggct ttaaaccaca gcaaattgcc gccacggaac
    25261 tgaacggcaa tacccgcgat ggcgtgattc acttcccgaa tatccgcact tccactctct
    25321 ggggtcaggt gcacgatgaa aaagcctttt attcgatggg cggcgtttcc gggcacgcag
    25381 gtttgttttc cgataccggc gatattgcgg tgttaatgca aacgatgctg aacggcggtg
    25441 gctatggtga tgtgcagctg ttcaatgcgg aaacggtgaa gatgttcacc accagctcta
    25501 aggaagatgc cacttttggc cttggctggc gcgtgaatgg taatgccacc atgacgccga
    25561 cgtttggcac gctggcaagc ccgcaaacct acggtcacac tggctggacc gggacggtga
    25621 ccgttatcga tccggtgaat catatggcga ttgtgatgtt aagcaacaag ccacattcgc
    25681 cagtagccga tccgcaaaag aatcccaata tgtttgaaag cggtcagttg ccgattgcaa
    25741 cttatggttg ggttgttgat caggtgtatg cggcgttaaa gcagtaataa aaaggccagt
    25801 cagttgtgat actggctggc ctttaatcat cgaatcagaa acgcttacaa cgccattaac
    25861 ttgtccagcg acggcgcgaa gtaatagccg ccagtgaccg gtttggtaaa acgcaacatc
    25921 gcatcacgtt tgccatcggt atcgccaaac atgctcagca gttgctgctc aatgttatgc
    25981 agacgcgcgc agtaggcgca gaagtacaga ccgtgcgtac cgctggcggt gccgtacggc
    26041 aggctctgtc gaacaatctt cagccctttg ccatcttctt tcagatcaac gcgggtcagg
    26101 tgagaggttt ccggacgttc gtcgccgtca atctcttcgt tggcctcttt agtgcgcccg
    26161 atcatcatct cttgatcgtg aatgctcatc cggttgagtt gcttcaggtt gtgctcccaa
    26221 cgctgcacaa acacatagct gccgccggca tccacgccat ctttgataac cgccacttcg
    26281 cggcgcgtct cttcacccgc cgggttttct gtaccgtcaa caaagccgct caggtcacgc
    26341 tcttcaaccc aacggaagcc gtggatctct tctttcactt caatgcaatc accaaacgct
    26401 tccatcgccg cctgggcgac agagaagttt acgtcgtgac gcagagaaag aatgtggatc
    26461 aacacatcga actgggtcgt cggtgcaaga cctttaccgt agcccggaaa atctttcagc
    26521 tcttcggccc caacgccgcc gctcagagcg cgccaggtat tgttaccaaa ggcaaccacc
    26581 gcaccaagat gcgcgtccgg gaatttcgct tcaaaagttg ccagcttgtc ggcaaatgtt
    26641 ttactggccg cacgcagggc gtcaacttcc cctttcacgt tggcttcgat ccaaatcgcc
    26701 gcgcggcaat gttctggcaa aatgccactc tgaacctgag acattgttcc tcctgaaaat
    26761 aagaatgcca cgcaatccgt ggcattgttg ggcgctattt tacgtgtttt ttcgcggcgg
    26821 cggtttgttc agacgcaaat taacgccgcc agataatttt actgactttc cagtttttca
    26881 gggtatcgtc cgaaggcatt aacccttcag gaccactcca ttccccgctg aactggtagc
    26941 taatatgttg actgccttcg gctttacatt cgacggcacg attatcatca ccatcagctt
    27001 tttggcaatt gccaaatgct ttgctgtaaa ggtcgctaaa cggtgtaccg attttaacac
    27061 cggtgtcagc aggaatatcg ctatccagca cgtcaatgcg actgatcgtg ccttgatcgc
    27121 cgttaatcac catcgccacg ttgtcgccct tcatcacttc aaaaaagcgc accacgttgc
    27181 cgttcgcggt tttcattccg ctgcgcaggc gataatcgcc atcaagcgca tcggcaatgg
    27241 cttgttcttg cagtggtgtg gacgccgtta attcaccaac gccctgctcg ctcactttgg
    27301 tggatgatcc aaaccagttc cacggattag cggcagacca gttaacggac gacatcgtgg
    27361 aacagccggt cagcatcaac ggcatagcgc ataacattaa acgcagcgat ttcatgtcac
    27421 ttcctttggt tattcaataa cgttgcttgg agtgcaaatt caccaaaaag tgccgttatt
    27481 ctttttcttc attaaaacaa gcgcgtaaac gtcgattggt cagcagccag attaacgcca
    27541 caatatccgc cactaccagc gccagaccga taccgctaac ggattcaccg ttcagccaca
    27601 accacggctg ccagcaaagt aaaaccactt gcgccaacag caacagaaaa tagagcacat
    27661 gccagatacg gggaaatgaa gcccgccgac cactcagcaa aaacgccagc accgccggaa
    27721 tgccgggaat caaccccagc cagaaattat cgtgatcggg ataaaacaga tttagcagtg
    27781 cagtaccctg ctcgcgcgac gcaccggcaa tgacaaacag cacccaggtt cgcgcctgaa
    27841 gcaatagcac aagccagaag agcaagggta aacgcaggcg accgtacgca tcataatgga
    27901 caggatgaaa ctcagtactc ttcatcttca atcaaacgct tacccagact cagcacgtcg
    27961 gcgtgttcat atcccaggcg ttcatacatt ccgagcacca tgtcgttatc ttccggcacg
    28021 ttgatctgaa ttttcgggca gccgcgagca atcagctttt tctccaaccg attaagcaac
    28081 gcattagcaa tcccacgccc acgaaactct ggatgcacgc caagataata agcagacccg
    28141 cgatgcccgt cataaccgcc catcaccgtt ccgaccactt caccgttcac ctcagcgacc
    28201 agaaacaaac tgacgtcatg gttcatctta cgctcaatgt ccatttccgg atcgttccac
    28261 ggacgcagca agtcgcaacg ctcccaaagg gtgatgacct cttcgaaatc ttcctggcga
    28321 aatacgcgta tctccatggt attcgttacc tttttgcggg ttaaaaggct gattatggcg
    28381 tgaacggtcg aattagccaa tatctgacga aaatcggttg aaaaagtggc ataatgggga
    28441 gttgtcaact attgaaatga aaagtaaaac aattctcaac agcaaacggt cgtaacggat
    28501 tacgcgatac gatataacat ctggaacttt attattacaa ctcaggccgt atgagcactt
    28561 ttaaaccact aaaaacactc acttcgcgcc gccaggtgct gaaagcagga cttgcagcgc
    28621 tgacgttgtc aggaatgtcg caagccatcg ccaaagaaga acctttaaaa accagcaacg
    28681 gacacagcaa gccgaaagca aaaaaatctg gtggcaaacg tgtcgttgtt ctcgatccag
    28741 gccacggcgg gattgatacc ggagcgatcg gacgcaacgg ttcaaaagaa aaacatgtgg
    28801 tgctggcaat tgccaaaaac gtccgttcca ttttgcgtaa tcatggcatt gatgcgcgtt
    28861 taacgcgttc tggcgatacg tttatcccac tgtacgatcg cgttgaaatc gcccataaac
    28921 atggcgcaga tctgtttatg tcaattcatg ccgacggctt taccaacccg aaagctgccg
    28981 gtgcttcggt atttgccctc tctaaccgtg gggcaagtag cgcaatggcg aaatacctgt
    29041 ctgaacgcga aaaccgcgcc gatgaagttg ccggtaaaaa ggcgactgac aaggatcacc
    29101 tattgcagca agtgctgttt gatctggtgc aaaccgatac tattaaaaac agcctgacgc
    29161 tcggctcgca tattctgaaa aagattaagc cggtgcataa actgcacagc cgcaacaccg
    29221 aacaagccgc gtttgtggtg ttgaaatcgc cgtcggttcc ttcggtgctg gtggaaacct
    29281 cgtttatcac caacccggaa gaagagcggc tgttaggcac ggcggcattt cgccagaaaa
    29341 tcgccacagc gattgccgaa ggcgtgatca gttatttcca ctggtttgac aaccagaaag
    29401 cacactcgag aaagcggtaa gtgatgaaac ccgacgcaca ccaggttaaa cagtttctgc
    29461 tcaaccttca ggatacaatt tgtcagcagt tgagcgccgt cgatggcaca gaatttgtcg
    29521 aagatagctg gcagcgcgaa gctggcggcg gcggacgtag ccgggtgttg cgtaatggtg
    29581 gtgttttcga acaggcgggt gtcaactttt cacatgtcca cggtgaggcg atgcctgctt
    29641 ccgccaccgc tcatcgcccg gaacttgccg ggcgcagttt cgaggcgatg ggcgtttcac
    29701 tggtagtgca tccgcataat ccgtatgttc ccaccagcca cgcgaatgtg cgctttttta
    29761 ttgccgaaaa accgggtgcc gaacccgtct ggtggtttgg cggcggcttc gacttaaccc
    29821 ctttctatgg ttttgaagac gatgctattc actggcaccg caccgcccgt gacctgtgcc
    29881 tgccatttgg cgaagacgtt tatccgcgtt acaaaaagtg gtgcgacgac tacttctacc
    29941 tcaaacatcg taacgaacaa cgcggcattg gcgggctgtt ctttgatgat ctgaacacgc
    30001 cagatttcga ccactgtttt gcctttatgc aggcggtagg caaaggctac accgacgctt
    30061 atttaccaat tgtagagcga cgtaaaacga tggcctacgg cgagcgcgag cgcaatttcc
    30121 agttatatcg tcgcggtcgt tatgtcgagt tcaatctggt ctgggatcgt ggcacactgt
    30181 ttggcctgca aaccggcggg cgcaccgagt ctatcctgat gtcaatgccg ccgctggtac
    30241 gctgggagta tgattatcag ccagaagatg gcagcccgga agcggcgtta agtgagttta
    30301 tta
//
LOCUS       DAZKGF010000039        30156 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZKGF010000039 DAZKGF010000000
VERSION     DAZKGF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.039"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHOQV_001731"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHOQV_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9630120.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHOQV_001732"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHOQV_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP9630121.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHOQV_001733"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHOQV_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP9630122.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHOQV_001734"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHOQV_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP9630123.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHOQV_001735"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHOQV_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP9630124.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHOQV_001736"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHOQV_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP9630125.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHOQV_001737"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHOQV_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP9630126.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHOQV_001738"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHOQV_001738"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9630127.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHOQV_001739"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHOQV_001739"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP9630128.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12916..14049)
                     /gene="thiH"
                     /locus_tag="ACHOQV_001740"
     CDS             complement(12916..14049)
                     /gene="thiH"
                     /locus_tag="ACHOQV_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFP9630129.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINSKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQVKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSISFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(14046..14816)
                     /gene="thiG"
                     /locus_tag="ACHOQV_001741"
     CDS             complement(14046..14816)
                     /gene="thiG"
                     /locus_tag="ACHOQV_001741"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFP9630130.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMMEAIRACGSQLVTLAMKR
                     VNLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIITQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14818..15018)
                     /gene="thiS"
                     /locus_tag="ACHOQV_001742"
     CDS             complement(14818..15018)
                     /gene="thiS"
                     /locus_tag="ACHOQV_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFP9630131.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQSGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15002..15757)
                     /gene="thiF"
                     /locus_tag="ACHOQV_001743"
     CDS             complement(15002..15757)
                     /gene="thiF"
                     /locus_tag="ACHOQV_001743"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFP9630132.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRNLALRRASGCPVCGGSNADPV"
     gene            complement(15750..16385)
                     /gene="thiE"
                     /locus_tag="ACHOQV_001744"
     CDS             complement(15750..16385)
                     /gene="thiE"
                     /locus_tag="ACHOQV_001744"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFP9630133.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16385..18280)
                     /gene="thiC"
                     /locus_tag="ACHOQV_001745"
     CDS             complement(16385..18280)
                     /gene="thiC"
                     /locus_tag="ACHOQV_001745"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFP9630134.1"
                     /translation="MSVTKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGREHIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18361..18461)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18513..18989)
                     /gene="rsd"
                     /locus_tag="ACHOQV_001746"
     CDS             complement(18513..18989)
                     /gene="rsd"
                     /locus_tag="ACHOQV_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFP9630135.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19084..19857
                     /gene="nudC"
                     /locus_tag="ACHOQV_001747"
     CDS             19084..19857
                     /gene="nudC"
                     /locus_tag="ACHOQV_001747"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFP9630136.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19897..20961
                     /gene="hemE"
                     /locus_tag="ACHOQV_001748"
     CDS             19897..20961
                     /gene="hemE"
                     /locus_tag="ACHOQV_001748"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFP9630137.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSAPYHL"
     gene            20971..21642
                     /gene="nfi"
                     /locus_tag="ACHOQV_001749"
     CDS             20971..21642
                     /gene="nfi"
                     /locus_tag="ACHOQV_001749"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFP9630138.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21685..22275
                     /gene="yjaG"
                     /locus_tag="ACHOQV_001750"
     CDS             21685..22275
                     /gene="yjaG"
                     /locus_tag="ACHOQV_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFP9630139.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22462..22734
                     /gene="hupA"
                     /locus_tag="ACHOQV_001751"
     CDS             22462..22734
                     /gene="hupA"
                     /locus_tag="ACHOQV_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFP9630140.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22747..23442
                     /gene="yjaH"
                     /locus_tag="ACHOQV_001752"
     CDS             22747..23442
                     /gene="yjaH"
                     /locus_tag="ACHOQV_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFP9630141.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23444..23863)
                     /gene="zraP"
                     /locus_tag="ACHOQV_001753"
     CDS             complement(23444..23863)
                     /gene="zraP"
                     /locus_tag="ACHOQV_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFP9630142.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMDHW"
     gene            24101..25477
                     /gene="zraS"
                     /locus_tag="ACHOQV_001754"
     CDS             24101..25477
                     /gene="zraS"
                     /locus_tag="ACHOQV_001754"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFP9630143.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGTGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQVGKGATFTLWLPVT
                     ITRKDPQG"
     gene            25474..26799
                     /gene="zraR"
                     /locus_tag="ACHOQV_001755"
     CDS             25474..26799
                     /gene="zraR"
                     /locus_tag="ACHOQV_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFP9630144.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLANHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENTVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26796..28085)
                     /gene="purD"
                     /locus_tag="ACHOQV_001756"
     CDS             complement(26796..28085)
                     /gene="purD"
                     /locus_tag="ACHOQV_001756"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP9630145.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28097..29686)
                     /gene="purH"
                     /locus_tag="ACHOQV_001757"
     CDS             complement(28097..29686)
                     /gene="purH"
                     /locus_tag="ACHOQV_001757"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP9630146.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLLLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAISNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7056 a         7829 c         8317 g         6954 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggtgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa tacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtagcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 aaccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagagttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgttgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggcgttgttg aagaagttga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga cccctgtaga gctggacttc agccaggttg
     1381 aaaaagctta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgcgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcactg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgagag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgctctgct gaaagaactg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 gggtgcaaac gctgaagctg ctaaagctgc aggtgctgaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggtaactg agtgagtcgc caggagcata gttaactatg
     2821 tgactggtgc gaacgaagga agccaacgcc gccacaagct gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggcttg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgt
     5281 gctgcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tattctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaata gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctatctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cctgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgacaagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgatatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccaga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggttac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcgtct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcatggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttctgg tcagatccgc
     7981 ctgtacgacg gccgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt actaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggtgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggcctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt acttcgaatc
     8881 ctatgtggtt attgaaggcg gtatgaccaa cctggaacgc cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaaa tgggtgcgga
     9001 agcaatccag gccctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gcagttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gtactgggtc tgtactacat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcctc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcta acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggctttg catatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtt attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa cggcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtact catgaaggta tcatgatgac
    10921 tccggttatc gaaggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg ttaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcaaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt acgcgtcaga ccgacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gatgtactga tcccgggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatctggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 tctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcactccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccaaaatg gcgtcagctc aatgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgatgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtta ctcgttacat cgttaacgaa gtacaagacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggtgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aactcgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaattctt cacggaattt ttaagaaagg cagggcgaaa
    12781 cgaggaagaa gcttttcgcc ctggacaaca ttcctgctga cgggactacc ataaaaaaac
    12841 acaacgcttt caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg
    12901 ttgttacatt ctgtctcatg gcctttgcga ggcgcgtccc agatagctgt cccagtcttt
    12961 ccataccggc tgcaaaccct gagcagttaa cgcggcagca accgcttccg gtctgcgatc
    13021 gtcgtgcggt gagaactgtt ccagctcggg gtgattgtcg gcatagccac ctggctgcgt
    13081 tttcgaaaaa gcgctgacgt tattaattgc cagcggaata acgcgatcgc gaaaccacgg
    13141 tgattcccgc gtggagagtg acagttcaat ctccggtgca agcagccgga aggcgcagat
    13201 ggtttgcact aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacatgg
    13261 acgcaggcgt gggaaggaga tagagtagcg gctttgccag taatgctgtt gcagccatag
    13321 caaatgttct gcaaccatat agcagtcaac gcgccagctg tcggaaaggc caattagcgc
    13381 gccgaggcct atcttatcaa tccccgcacg gcccagccga tccggcgttt ccagccgcca
    13441 gaagaagtcc tgttttttac ctttcagatg atggcgggca taagtcgcct cgtgatatgt
    13501 ctcctgataa accatcacgc catccagacc tagttgcttt aactcggcgt attccgtctc
    13561 cgccagcggt tgcacttcca tctgtagtga agagaactgt tcacgcaggg cagggagatg
    13621 acgacgaaag taatccatcc ccactttcac ctgatgttca ccagtgacta atagcagatg
    13681 ttcaaagccc atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt
    13741 tttgcgcttg atgcgattgc tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag
    13801 attggaaaga taaagcggga cgtagaagct gacgacgttg ccaaatcgct gacgggtcag
    13861 acgctgcgcc cgttgggcca gtggttccag atagccactg gcggctggcg ataacagcgc
    13921 catcatatcg tcgcgggtga gttgcgaggc atttagcgcc cgctctacgt cagcagccgt
    13981 tttgctgttg atacgcaggc ggatgtcgtc ccagtccagt tgtcgccagc gatcgctgaa
    14041 ggttttcatg ctgatgcctc cagaaatccg gtcagcgggc tggtggcatg agcaaaatga
    14101 ctgcggctgc ccggtccgga ctgacgtgcc agcaggcctg cttctactgc cagacgaaat
    14161 gccttcgcca tgttgacggg atcgtccgcg acggcaatcg ccgtattcac taacaccgcg
    14221 tcggccccca tttccagcgc ctgcgcggca tggctgggaa cgccgatgcc agcatcgaca
    14281 accaccggaa ccgtggcttg ctgggtaata atctccagca tggcacgggt ttccagtccc
    14341 tgattcgagc caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga
    14401 cgtttacaca ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc
    14461 agcgtttcgg cggctttcag ggtttcgatg ggatcgggca acagccagcg ggcgtcaggg
    14521 tgaatctcta attttaacca gtttgtgcct aacgcttcac gagccagatg ggcggcgaaa
    14581 atggcttctt ccgctgtttt cgccccggat gtatttggca gcagggtcac acccgccgcg
    14641 ataagcggtt cgaggatagc gtcgttgtgc tggcgcaagt tgacgcgttt catcgccagt
    14701 gtcaccagtt ggctgccgca agcgcggatc gcctccatca tcagttgtga agaggcgaat
    14761 ttccctgtgc cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca
    14821 accccctgca ataacctgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgc
    14881 ccactgctca cgcgggacga tttgctgatt aatcgccaga gccgcgcccg attgtcgttg
    14941 gtcgagttgc tccagtagtt cgtgaaccgt ttgcccggcg gcgcactgca tcggttgatc
    15001 gttaaacagg atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg
    15061 ccaggttgcg ccactggctc gatttaccgt cgaacagtcg gagttctccc gcaggcgtct
    15121 ctataccgct taataactta atggcttcca gtgcctgtaa agtgcccata accccgacca
    15181 ccgggccaac cacgcccgcc gtgcggcagt tgcgttcagg ctcctggtta tctggccaca
    15241 ggcagcggta acacccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc
    15301 cgaccgcgct ggcggtgata agcggcgtgt tgagtgccac gcaggcggca ttaatctcct
    15361 gacgagtcgc catattgtcg gtacagtcga gcaccacatc ggcccgtgct accgcatctt
    15421 ttaacgcctc acccgttaac cgttgttgta atgctgtcag ttgaatatcg ggattcaact
    15481 gtgtcagtcg ctgatggctg acctgcgatt tcgggcgatc gatatcttca gtggtaaaga
    15541 ggatttgtcg ttgcagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc
    15601 cgacgccagc gcccgccagg tatagcgcag caggtgtacc cagcccgcca agaccgataa
    15661 tcagcacctg gctgtcgagc agtttttgct gcccgtcaag agcgatatcg tcgagcagga
    15721 tttggcggct ataacgcata aagtcacggt cattcatcgc caactcctgc aatttccagc
    15781 aactgtgccg ttgccaaacg ccagtctgcg gcttgagtaa tggcgcttac gacggcgatg
    15841 ctgccgacac ccgttgctat caccgcaggc gcgcgcgcca gactgatacc gccaatcgcc
    15901 acggtgggat aatccgccag gcgctcaaca tgccgtgcca gctgttccag cccctgcggt
    15961 gcagaaggca tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg
    16021 cgtgctgcca gcgcgacgtc gatctccata tcgtcatggg tcgaaacgcc cagtcgcaga
    16081 cctgccgcgc ggatggcgct gagatcggtg gcttgcaaat cttcctgccc cagatggacg
    16141 ccatatgcct gatgcttgat cgccagccgc cagtaatcgt tgataaacaa tcgcgcgtta
    16201 tagcggcggc ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgtcga
    16261 tctttgatgc gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt
    16321 acgctgtcca ccaccgggta cagtcctaaa cgaaagggta caggaggaaa atctggctga
    16381 tacatcacgc ttcctcctta cgcaggtaga tttctccgcc tctggcgcgg aagttctccg
    16441 acatatcagc cattcccact tcaatggttt gcgcggcggc gtaatcgcgc acttcctggc
    16501 tgattttcat cgagcagaat ttcggcccac acatggagca aaaatgggcg actttgccgg
    16561 attcttgcgg cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat
    16621 taaactggtc ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt
    16681 gcgcgcccgg atgccctttc gccaggtcag cggcgtgggc ggcaatctta taggtgataa
    16741 gcccctgctt aacatcttct ttattaggca gacccagatg ctcttttggc gttacgtaac
    16801 agagcatcgc gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt
    16861 cataacccgg cgcgatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc
    16921 agtgctctaa ctcctcggtc atattgcggc ggatcatctg catcggcacg tggcctgggc
    16981 cttcaatcat cacctgtacg tcatattccc aggcgatttt ggtcagttca cccagcgtat
    17041 gcagctcggc aaattgcgct tcatcgttgg cgtcctgaat agaaccaggg cgcagaccgt
    17101 ctcccagcga cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt
    17161 gatagaggaa attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc
    17221 gagagacgat accggtcagg cgtttcgcgg tcatcggcac atagcgcagc agtacgcccg
    17281 catggatagt gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg
    17341 cttcccaggt aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca
    17401 ctgtaccgat cggcaccggg ctgttacgca aaatccactc gcgggtttcg tgaatatagc
    17461 gaccggtgga gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt
    17521 ccacttcttc ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta
    17581 ccaggaaatt gcgaccaata atcatcggct ccgattccgg atgattaatg ttggccggga
    17641 taatcgcgcg tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca
    17701 gacgtgcgcc aaagctcatt cccggatgct ggtggcgtaa aacttcgcta cgtatgtgct
    17761 cgcggcccat attctcgcgg atggcgatga attccatttc cggtgtgatg atgccctggc
    17821 gggcgtagtg cagttgggtg acgcggcgtc ctgctttggc gcgttttggt gttagtacgc
    17881 cgctaaaacg cagctcgtcg aggccatcat ctgccagccg cgctttagtg taatcggaac
    17941 tgcgcacggt aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg
    18001 ccagtccttg ctgcacgtta atggcgatct gtggatcgcc atacggaccg gaggtgtcgt
    18061 agaccggaat cgcttcgttt tcttcgtact gcggttgttc tttgctgccg ccaattagcg
    18121 tcgggctaag ctggatctca cgcatcggca cgcgcacgcc ggggtgtgtg ccagtgatgt
    18181 aaatgcgttt tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggcct
    18241 gggcgcgttg ttcgcggcgg gtcagttttg ttacagacat agctcattcc aaaaagttaa
    18301 ggacgtggct tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat
    18361 agcagattaa ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga
    18421 ataaacggtc tcagccagtt aaggcactcc gacaagaaat tagccccaca aacggggcgt
    18481 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18541 tcaagtacca gcagaatcaa cttatcttcc agcacaaagc gtgcttccag cgcctcgccg
    18601 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    18661 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    18721 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    18781 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    18841 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    18901 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgt
    18961 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19021 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19081 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19141 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19201 ggtcagcgcg cactacaaat cggcgaatgg caaggggaac ctgtttggtt agtacaacag
    19261 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt agggctgttt
    19321 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19381 tactgcgggc atgaaatgta tccaagcaaa accgaatggg cgatgctgtg cagccattgc
    19441 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19501 tcgatcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19561 ttcgtcgaag tgggtgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19621 ggaattaaag ttaaaaactt gcgttacgtg acctcccaac cgtggccgtt tcctcagtcg
    19681 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgttatcga cccgaaagaa
    19741 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    19801 gtagcgcgcc gtctgataga agatacggtg gcgatgtgtc gggcagagta tgagtgatga
    19861 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    19921 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    19981 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20041 ctgtgcaaaa atgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctatccg
    20101 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cagacgcgat gggtttaggg
    20161 ctctattttg aagccggaga aggtccgcgt tttacctcac cagtcacctg caaggccgac
    20221 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20281 cgtaccattc gtcgcgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20341 tggacgctgg cgacctatat ggtggaaggc ggcagcagca aagcgttcac cgtgatcaaa
    20401 aaaatgatgt atgccgatcc acaggcgctg cacgcgctac tcgataaact ggcgaaaagc
    20461 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggctgtgat gattttcgac
    20521 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20581 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtaccggt cacgctgttt
    20641 accaaaggcg gcggacagtg gctggaagcc atggcagaaa ccggttgcga tgcgctgggc
    20701 ctcgactgga caacggacat cgccgatgca cgccgccgcg tgggcaataa agtcgcgttg
    20761 cagggcaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    20821 gcgactatac ttgcaggttt cggtcacggt gaaggtcatg tctttaacct tggtcacggc
    20881 attcatcagg atgtgccgcc agaacatgct ggcgtgtttg tggaggcagt gcatcgcctg
    20941 tctgcgcctt atcatctgta aggagtggtt atggatctcg cgtcattacg cgctcaacaa
    21001 atcgaactgg cttcttctgt gatccgcgag gatcgactcg ataaagatcc accggatctg
    21061 atcgccggag ccgatgtcgg atttgagcag ggcggagaag tgacgcgagc ggcgatggtg
    21121 ctgctgaaat acccctcgct tgagctggtg gagtataaag tggcccgcat cgccaccacc
    21181 atgccttaca ttcctggttt tctctccttt cgtgaatatc ctgcgctgct ggcagcgtgg
    21241 gagatgctgt cacaaaagcc ggatttagtg tttgtcgatg gtcatgggat ctcgcatcct
    21301 cgccgtcttg gcgtcgccag ccattttggt ttgctggtgg atgtgccgac cattggcgta
    21361 gcgaaaaaac ggctctgcgg taaattcgaa ccgctctcca gcgaaccggg cgcgctggcc
    21421 ccgctgatgg ataaaggcga gcagctggcc tgggtctggc gcagcaaagc gcgctgtaac
    21481 ccattgttta tcgctaccgg ccatcgggtc agcgtggaca gcgcgctggc gtgggtacaa
    21541 cgctgcatga aaggctatcg tctgccggag ccaacgcgct gggcggacgc ggtggcctcg
    21601 gaacgtccgg cgttcgtgcg ctatacagca aatcagccct aattcaggta aactgcggat
    21661 aatttccgta tttgagaact caacatgtta caaaacccaa ttcatctgcg tctggagcgc
    21721 ctggaaagct ggcagcacgt cacttttatg gcctgcttat gcgaacgtat gtaccccaat
    21781 tacgccgtat tctgtcagca aaccggcttc ggcgatgggc agatttaccg ccgcattctc
    21841 gatctcattt gggaaacgct gaccgtcaaa gacgcaaaag taaatttcga cagccaactg
    21901 gagaaatttg aagaagcgat tccttcagcc gacgatttcg atctgtacgg cgtttatccg
    21961 gcaatcgatg cttgcgtggc gttaagtgaa ctggtccatt cgcgtttaag tggcgaaacg
    22021 ctcgaacacg cggtggaagt gagtaagact tccatcacga ccgtggcgat gctggaaatg
    22081 actcaggctg gtcgcgaaat gagcgatgaa gagctcaaag aaaacccagc tgtagagcaa
    22141 gaatgggaca ttcagtggga aatattccga cttttagctg agtgcgaaga acgcgatatc
    22201 gagctgataa aaggccttag ggcagacctg cgtgaggcgg gtgagagcaa tattggtata
    22261 atttttcagc aataagacca gaaaacgtga tttaacgcct gatttgtcgt acctggagtc
    22321 ttccctttcg ccccccgtct ggtctacatt tggggggcga aaaaaagtgg ctatcggtgc
    22381 gtgtatgcag gagagtgcta ttctggcatt tccgtcgcac tcgatgctta gcaagcgata
    22441 aacacattgt aaggataact tatgaacaag actcaactga ttgatgtaat tgcagagaaa
    22501 gcagaactgt ccaaaaccca ggctaaagct gctctggagt ccactctggc tgcaattact
    22561 gagtctctga aagaaggcga tgctgtacaa ctggttggtt tcggtacctt caaagtgaac
    22621 caccgcgctg agcgtactgg ccgcaacccg cagaccggta aagaaatcaa aattgccgca
    22681 gctaacgtac cggcatttgt ttctggcaag gcactgaaag acgcagttaa gtaagattgc
    22741 gtggcagtga acagttttaa cgaaggggtg gtttcacccc ttttgtcttt ctggcgtcga
    22801 tcattgatgc tggctggcgc tctgtttctc actgcttgta gtcacaactc ttcacttcct
    22861 ccctttaccg ccagtggatt tgctgaagac cagggcgcgg tacgcatctg gcgaaaagac
    22921 agcggcgata atgtgcatct tcttgccgtg tttagcccgt ggcgcagtgg cgatacgacg
    22981 acgcgagagt atcgctggca gggcgataac ctcacgctca tcaatatcaa tgtttacagc
    23041 aaacccccgg tgaatattcg cgcgcgtttt gacgatcgcg gcgatctgag ctttatgcaa
    23101 cgcgaatccg atggggaaaa gcagcagctt tctaacgacc aaatcgattt ataccgttat
    23161 cgtgctgatc agatccgcca gattagcgat gccttacgtc aggggagagt cgtgctgcgc
    23221 caggggcgct ggcatgcgat ggagcagact gtgaccacct gcgaagggca aaccattaaa
    23281 cctgatttag attcgcaggc gatagcgcat atcgagcgcc gccagagccg ctcttctgtt
    23341 gatgtcagcg tggcatggct ggaagcgccc gaaggttcgc aattactgtt ggtggcaaac
    23401 tctgatttct gtcgctggca acccaacgag aaaacgttct gatttaccag tggtccatac
    23461 ccatatgacc gccaccaccg cagccgccgt aacccattcc ggtaccgcgc ggaatacccg
    23521 cttcagccat cgcaatatct cgtttcaccc gtaactcatc taacgactga cgcaaattct
    23581 ccatctcttt ggcgaccgcg ttaattttgc tgctatccgg tgggttcgcg gctaacaggg
    23641 cattgtattc ataacgcttt gttaccagtt gctgttgcag tgcgctgctt tgagcgtaaa
    23701 agtcattatg gattttctgc cacgctgtct gttgttcgct ggtcaaaggc gcggcatttt
    23761 gctgccacat accgtgtccg ccgtgagcaa atgccgatgt cgatcccatc gccattgctg
    23821 aaagcgccat cattaccaag gcaattttcg tgttccgttt catggttaat cctccagtgg
    23881 ttgtcttact tcgggtattg catcttccgt gccaacgatg aaacgctgat atgacgggta
    23941 atctggcatg ataaacgagt aaaaatgact cgcctgctgc gggtagcgag tcatttttac
    24001 tcattgaaac ttaagccttt gtgttacagc gcagggtaag cgctgataaa agatggcatg
    24061 atttctgctg tcagaaaggg atgagcaggc aaagaagaag atgcgtttta tgcaacgttc
    24121 taaagactcc ttagctaaat ggttaagcgc gatcctcccc gtggtcattg ttgggctggt
    24181 ggggctgttt gcggtgacag tgattcgcga ttacgggcgc gagactgcag ccgccagaca
    24241 aacgctgctg gaaaaaggca gtgtacttat ccgtgctctc gaatccggct cgcgcgtcgg
    24301 catggggatg cgcatgcatc atgcgcagca gcaggcatta ctggaagaaa tggccgggca
    24361 gcctggtgta cgttggtttg cggtcacgga tgaacaagga acaatcgtga tgcatagcaa
    24421 ctccggcatg gtgggaaaac agctttattc cccgcaggaa atgcagcagt tacatccggg
    24481 agatgaagaa gcgtggcggc ggatcgatag cgcagacggc gagcctgttc tggaaattta
    24541 tcgccagttt caaccgatgt ttgctgctgg aatgcaccgg atgcgccata tgcagcagta
    24601 tgccgcgaca ccacaagcaa tttttatcgc tttcgacgcc agtaacattg tgagtgccga
    24661 agatcgtgag cagagaaaca ccctgattat cctcttcgcc ctggcgacgg tcttgctggc
    24721 aagcgtgttg tcattcttct ggtatcgccg atatctgcgc tcgggccagc tgttgcagga
    24781 tgaaatgaag cgcaaagaga agctggtggc actggggcat cttgcggcag gcgttgccca
    24841 cgaaatccgt aacccacttt cctcgattaa agggctggcg aaatactttg ccgaacgcgc
    24901 gccagcaggg ggagaagcgc atcaactggc gcaggtgatg gcgaaagaag ccgaccgttt
    24961 aaaccgcgtg gtaagcgagt tgctggaact ggttaagcca acgcatctgg ctttgcaggc
    25021 ggtggatctc aacacgctga ttaaccactc attacagctg gtaagccagg atgcaaacag
    25081 ccgggagatc cagttacgct ttaccgccaa cgacacatta ccggaaattc aggccgatcc
    25141 ggacaggctg actcaggtcc tgttgaatct ctatctcaat gctattcagg cgattggtca
    25201 gcatggcgtg attagcgtga cggccagcga aagcggcacg ggtgtgaaaa tcagcgttac
    25261 cgacagcggt aagggaattg cggcggatca gcttgaagcc atcttcaccc cgtatttcac
    25321 cacgaaggcc gaaggcaccg gactggggct ggcggtcgtg cataatattg ttgaacaaca
    25381 cggtggtaca attcaggtcg caagccaggt gggaaaaggc gcaacgttca ccctctggct
    25441 tccggtcact attacgcgta aggacccaca aggatgacgc acgataatat cgatattctg
    25501 gtggtggatg atgacattag ccactgcact attttgcagg ctttgctgcg cggctggggc
    25561 tataacgtcg cgctggcgaa cagcgggcga caggcgctgg agcaggtgcg ggaacaggtt
    25621 tttgatcttg tgctttgcga tgtgcgaatg gcggagatgg acggcattgc cacgctaaaa
    25681 gagatcaaag cgctaaatcc ggcgattccg gtactgatta tgaccgcata ctccagcgtc
    25741 gagacggcgg tagaggcact gaaaaccggg gcgctggatt atctcatcaa gccgctggat
    25801 ttcgataacc tgcaggcgac gctggaaaag gcgctcgcac atacgcacag tgttgacgct
    25861 gaaacgcctg cggtgtctgc cagccagttc ggtatggtag gtaaaagccc ggcgatgcaa
    25921 cacctgctca gtgaaatcgc cctcgtcgcg ccatcggaag ccacagtgct gatccacggc
    25981 gattccggca ccggtaaaga gctggtcgca agggcgattc acgccagtag cgcacggagt
    26041 gaaaaaccac tggtaacgct caactgtgcg gcactcaacg aatccttgct ggaatctgaa
    26101 ttgttcggtc acgaaaaagg ggcgtttacc ggagccgata aacgccggga agggcgcttt
    26161 gttgaggcgg acggcggcac gctgtttctc gatgaaattg gcgatatctc gccgatgatg
    26221 caggttcgtc tgctgcgtgc gattcaggag cgcgaagttc agcgtgtcgg cagcaaccag
    26281 accatctcgg ttgatgtccg gctgattgcg gcgactcatc gcgatcttgc cgcagaggtg
    26341 aatgccgggc gttttcgcca ggatctctac tatcgcctga acgtggtggc gattgaagtt
    26401 ccgtcgctgc gtcagcggcg ggaagatatt cctctgctgg caaaccattt tcttcagcgc
    26461 tttgccgagc gtaatcgtaa ggcggtaaaa ggttttacgc cccaggcgat ggatctgctg
    26521 attcactacg actggccggg aaatattcgt gaactggaaa acacggtgga acgggcggtg
    26581 gtgctgctga ccggggaata tatttccgaa cgcgagctgc cgctggcgat tgcgagtacg
    26641 ccgatcccgc tggtacaaag tcaggatatt cagccgctgg tggaagtcga aaaagaggtg
    26701 attctggcgg cgctggagaa aacgggcggc aacaaaaccg aagccgcccg ccagttaggg
    26761 atcacgcgca aaacgctatt ggcaaaactg tcgcgttagt tctgctcgcg ttcgatagcg
    26821 cgccagccga tatctttccg gcagaagcag tcgtcccaat ggatatcggt cattaaggca
    26881 taagcgcgtt tctgcgcttc tgccacggta tgacccagcg cggtaacgca cagtacgcgc
    26941 ccgccgctgg tcactacctg ctcgtcatcc gctagtttcg tgcccgcgtg gaacactttg
    27001 ccgtctgcca cttcttccag cggcaggccg tggatcacat cgccggtgcg gtaatcgccc
    27061 ggatatccac ccgcagccat caccacgccg agagaagcgc gttcatccca ctcggacgtt
    27121 ttctcgtcca gcttgccttc acaggccgcc aggcagagct caaccagatc ggacttcatg
    27181 cgcagcataa tcggctgggt ttctggatcg ccaaagcggc agttaaattc aataaccttc
    27241 ggattgccct gtttgtcgat catcaggccc gcgtagagaa aaccagtgta ggtgttgcct
    27301 tctgccgcca tgcctttcac ggttggccag atgattcgtt ccatcgtgcg ctgatgaact
    27361 tcatcggtta ctaccggcgc gggggagtaa gcgcccatcc cgcccgtgtt tggtccggta
    27421 tctttatcgc ctacgcgttt gtgatcctgg ctggtagcca tcggcagcac atgctcgccg
    27481 tccaccatca cgataaagct cgcttcttcg ccatcgagga actcttcgat aacgatgcga
    27541 tgacccgcgt cgccaaacgc gttgcccgcc agcatatcgt gaacagctgc ttcagcttct
    27601 tccagcgtca tcgccacgat aacgcctttc ccggctgcca gaccgtcagc tttaatgacg
    27661 attggcgcgc ctttctcacg cagatacgcc agcgcaggtt ctacctcggt gaagttctgg
    27721 tattccgctg tgggaatgtt atggcgggcc agaaagtctt tggtgaatgc tttagaacct
    27781 tccagttgtg cggcacctgc ggttgggccg aagattttca gcccggcggc gcggaaggta
    27841 tcgactacgc ctttcaccag cggcgcttcc gggccgacga tggttagatc gatcttttcg
    27901 ttttgcgcga aatccagcag cgccgggata tcggttacgc cgatagcgac gttttgcagt
    27961 gtgggttcca gcgcagtgcc tgcattaccc ggagcaacaa aaacagtttc aaccagcggc
    28021 gactgggccg ctttccaggc cagcgcgtgc tcgcgcccgc cgttaccaat cactaatact
    28081 ttcatctgtc gctccattaa tggcggaagt ggcgcatgtc ggtgaagagc atcgcaatac
    28141 cgtgctcgtc ggcggcggca atcacttcgt catcacggat agaaccgcca ggctggatta
    28201 cgcaggtcac acccgcagcg gcggcggcat caataccgtc gcggaacggg aagaacgcgt
    28261 cagaagccat cgaggaacct ttcacttcca ggccttcatc ggccgcttta ataccggcga
    28321 tttttgcgga gtacacgcgg ctcatctggc ccgcgccaat gccgatggtc atattgtttt
    28381 tggcatagac aatggcgttg gatttcacga acttcgccac tttccagcag aacagcgcat
    28441 cacgcagttc ctgttcggtc ggctgacgtt tggtgacgac gcgcagttct tctgcaccga
    28501 ccatgcccag gtcgcgatcc tgaaccagca gaccgccgtt aacacgtttg aaatcaagac
    28561 ccggaacacg ctcgccccac tgaccgcagg tcaggacgcg tacgttctgt ttggcggcgg
    28621 tgattttcag ggcttcttcg ctggcggacg gcgcaataat cacttcaaca aactgacgag
    28681 aaatgatggc ctgcgcggtt tccgcatcca gctcgcggtt aaaggcgata atgccgccga
    28741 atgcggaggt cgggtcggtt ttgtacgcgc gatcgtaagc atcaagaatg gaattgctga
    28801 tagccacgcc gcacgggttg gcgtgcttca caatcacaca tgctggctcg gcgaactctt
    28861 tcacgcactc cagcgccgca tcggtatcgg cgatgttgtt ataagagagg gccttaccct
    28921 gaacctgggt tgcggtggca acagaggctt ctttcacgtt ctcttctata tagaaggcag
    28981 cctgctggtg gctgttctcg ccgtaacgca tatcctgctt cttaatgaag ttcaggttca
    29041 gcgtgcgtgg gaagcgaccg gcggcttctt tgctttcacc gtggtaagcc ggaaccatgc
    29101 tgccgaagta gttggcaatc atgctgtcgt aggcggcggt atgttcaaag gctttgatag
    29161 cgagatcgaa acgggttgca agcagaagcg atccttcgtt ggcatccatc tctttaatga
    29221 tggcgtcgta gtcgctgctc ttcaccacga tggcgacatc tttatggttc ttggcggcag
    29281 agcgcaccat cgtcgggccg ccgatatcga tgttctcaac cgcatcttcc agcgagcaac
    29341 cttcacgggc aacggtctgg gcgaacggat acaggttaac aaccaccata tcgataggct
    29401 ggatctgatg ttcttccata atggcatcgt cctggccgcg acggcccaga atgccaccat
    29461 gtactttcgg atgcagggtc ttcacgcgtc catccatcat ctccgggaaa ccggtgtaat
    29521 cggaaacttc ggttaccggc agaccttttt ctgctaacag acgggctgtg ccccctgtag
    29581 acagcagctc cacaccgcgt gcggaaagtg cctgggcgaa ttcgacgata ccggctttgt
    29641 cagaaacact gagcagagcg cggcggactg gacgacgttg ttgcatggta aatcccctgg
    29701 atttgactat tacagagagc gttagctgaa tttttcgcga aaaactcagc taacgcccct
    29761 aacggggcat ccttattttt cgcccgcatt gtaacgaaaa cgtttgcgca acgctcgcga
    29821 atttttctct ttcaatggtg atcacaattt tgacagtggc taccgtggga aaaatacaga
    29881 aattacattg atgattgtgg ataactctgt gcgtaaaaag gtataaagcg gggttttgct
    29941 ggggaatgca gcagtcagtc atttttatgc aatttttcta ttgcgacctg cggagaactc
    30001 cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
    30061 ggttgaagag aaaaatcctg aaattcaggg ttgactctga aagaggaaag cgtaatatac
    30121 gccacctcgc gacagtgagc tgaaagccgc gtcgca
//
LOCUS       DAZKGF010000040        30077 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZKGF010000040 DAZKGF010000000
VERSION     DAZKGF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.040"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1023)
                     /gene="eutR"
                     /locus_tag="ACHOQV_001758"
     CDS             complement(<1..1023)
                     /gene="eutR"
                     /locus_tag="ACHOQV_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFP9630147.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLT"
     gene            complement(1069..1569)
                     /gene="eutK"
                     /locus_tag="ACHOQV_001759"
     CDS             complement(1069..1569)
                     /gene="eutK"
                     /locus_tag="ACHOQV_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFP9630148.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(1582..2241)
                     /gene="eutL"
                     /locus_tag="ACHOQV_001760"
     CDS             complement(1582..2241)
                     /gene="eutL"
                     /locus_tag="ACHOQV_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFP9630149.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(2251..3138)
                     /gene="eutC"
                     /locus_tag="ACHOQV_001761"
     CDS             complement(2251..3138)
                     /gene="eutC"
                     /locus_tag="ACHOQV_001761"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFP9630150.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCTTPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(3159..4520)
                     /gene="eutB"
                     /locus_tag="ACHOQV_001762"
     CDS             complement(3159..4520)
                     /gene="eutB"
                     /locus_tag="ACHOQV_001762"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFP9630151.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(4532..5935)
                     /gene="eutA"
                     /locus_tag="ACHOQV_001763"
     CDS             complement(4532..5935)
                     /gene="eutA"
                     /locus_tag="ACHOQV_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFP9630152.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTEARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPVGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDEKDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(5932..7158)
                     /gene="eutH"
                     /locus_tag="ACHOQV_001764"
     CDS             complement(5932..7158)
                     /gene="eutH"
                     /locus_tag="ACHOQV_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFP9630153.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDASAAKTEAEAQS"
     gene            complement(7375..8562)
                     /gene="eutG"
                     /locus_tag="ACHOQV_001765"
     CDS             complement(7375..8562)
                     /gene="eutG"
                     /locus_tag="ACHOQV_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFP9630154.1"
                     /translation="MQSELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSLVGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(8552..9388)
                     /gene="eutJ"
                     /locus_tag="ACHOQV_001766"
     CDS             complement(8552..9388)
                     /gene="eutJ"
                     /locus_tag="ACHOQV_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFP9630155.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKTEGIY
                     AK"
     gene            complement(9399..10802)
                     /gene="eutE"
                     /locus_tag="ACHOQV_001767"
     CDS             complement(9399..10802)
                     /gene="eutE"
                     /locus_tag="ACHOQV_001767"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFP9630156.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(10814..11101)
                     /gene="eutN"
                     /locus_tag="ACHOQV_001768"
     CDS             complement(10814..11101)
                     /gene="eutN"
                     /locus_tag="ACHOQV_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFP9630157.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(11208..11501)
                     /gene="eutM"
                     /locus_tag="ACHOQV_001769"
     CDS             complement(11208..11501)
                     /gene="eutM"
                     /locus_tag="ACHOQV_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFP9630158.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(11540..12556)
                     /gene="pta"
                     /locus_tag="ACHOQV_001770"
     CDS             complement(11540..12556)
                     /gene="pta"
                     /locus_tag="ACHOQV_001770"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9630159.1"
                     /translation="MIIERCRKLALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFALRWLARAGEKTPPDALDKLTD
                     PLMFAAAMVSAGTADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVQRQTEVNRESSLQ
                     TLVE"
     gene            complement(12553..13356)
                     /gene="eutT"
                     /locus_tag="ACHOQV_001771"
     CDS             complement(12553..13356)
                     /gene="eutT"
                     /locus_tag="ACHOQV_001771"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFP9630160.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLAFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGE
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQSLTVKQIQQRLGETQ"
     gene            complement(13353..14054)
                     /gene="eutQ"
                     /locus_tag="ACHOQV_001772"
     CDS             complement(13353..14054)
                     /gene="eutQ"
                     /locus_tag="ACHOQV_001772"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFP9630161.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(14029..14508)
                     /gene="eutP"
                     /locus_tag="ACHOQV_001773"
     CDS             complement(14029..14508)
                     /gene="eutP"
                     /locus_tag="ACHOQV_001773"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFP9630162.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(14521..14856)
                     /gene="eutS"
                     /locus_tag="ACHOQV_001774"
     CDS             complement(14521..14856)
                     /gene="eutS"
                     /locus_tag="ACHOQV_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFP9630163.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(15149..17428)
                     /gene="maeB"
                     /locus_tag="ACHOQV_001775"
     CDS             complement(15149..17428)
                     /gene="maeB"
                     /locus_tag="ACHOQV_001775"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFP9630164.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYRGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            17717..18667
                     /gene="tal"
                     /locus_tag="ACHOQV_001776"
     CDS             17717..18667
                     /gene="tal"
                     /locus_tag="ACHOQV_001776"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP9630165.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            18687..20690
                     /gene="tkt"
                     /locus_tag="ACHOQV_001777"
     CDS             18687..20690
                     /gene="tkt"
                     /locus_tag="ACHOQV_001777"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9630166.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKYAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(20793..21836)
                     /gene="ypfG"
                     /locus_tag="ACHOQV_001778"
     CDS             complement(20793..21836)
                     /gene="ypfG"
                     /locus_tag="ACHOQV_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFP9630167.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNSGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(21962..22537)
                     /gene="nudK"
                     /locus_tag="ACHOQV_001779"
     CDS             complement(21962..22537)
                     /gene="nudK"
                     /locus_tag="ACHOQV_001779"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFP9630168.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     NKALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(22605..24584)
                     /gene="aegA"
                     /locus_tag="ACHOQV_001780"
     CDS             complement(22605..24584)
                     /gene="aegA"
                     /locus_tag="ACHOQV_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFP9630169.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QVQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVEVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            24790..26490
                     /gene="narQ"
                     /locus_tag="ACHOQV_001781"
     CDS             24790..26490
                     /gene="narQ"
                     /locus_tag="ACHOQV_001781"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFP9630170.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            26654..29767
                     /gene="acrD"
                     /locus_tag="ACHOQV_001782"
     CDS             26654..29767
                     /gene="acrD"
                     /locus_tag="ACHOQV_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFP9630171.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAQLVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(29866..29925)
                     /gene="ypfM"
                     /locus_tag="ACHOQV_001783"
     CDS             complement(29866..29925)
                     /gene="ypfM"
                     /locus_tag="ACHOQV_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFP9630172.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         6794 a         8488 c         7878 g         6917 t
ORIGIN      
        1 cgtcagtgac ggcttctcgg caaacagctg ctggtaatcc gtggcgaatt gccccagatg
       61 ccagaatccc cactgcatgg cggcgtcttt taccgttgtg ctttgcgacc aagggcttat
      121 cagttcgcgg cgtacggcgt tcaggcgaat gcgtttcagc cacgcgtttg ggccaatgcc
      181 taaaatagcg tgaaacgcgt tttgtagcgt gcggcggctg acatgcagtt gattacacaa
      241 gtccagcacc gtcactggtt cggacatgtt ttccagcaca tattcacggg cgcgggaaag
      301 caatcgacgg taactctgat gactgatgct ttccgccgtc accattggct gcgcttcttc
      361 cagcatcgcc cccatcgcca ttagcaaatt atcccccagc acttttcgca ctgcaggctg
      421 atggagattt tccgggtttt cgcaaaacgt cgccagcgcc tgttggacaa agccccacag
      481 agcggcttta tgctgctctt tcacttccaa cgccgactgg ctacgcagca tatgtaatac
      541 ccgatccgga ttatgcaaaa agttagcctg acgagtgatg acatcttcag aaagcactac
      601 gccaaggatc gtgtaatcgt ccggcgtact taattcaaac tcggttccac cggggcgcgt
      661 cgctatttct gcgcttccca gacattgcga accgataaat ccctgctcac cgcgcgtcgc
      721 cggaatgcca aaccagaacg agttcggcca gaccaggcac gactggcgca gcgccagacc
      781 ggtgtattca cggaaaacct gaatatcatc gagtagaatt tccgtaaatt caccatgaaa
      841 cttgcccgga tgcagctgat cgtaaatctg ctgccaggcg gtaattgtca aagcatgttc
      901 atagacatcc gttgtctgtc gttgatgaac attatccact tcgaccttcg gcgtgagctt
      961 caggttttcg ggtaagggtt catgataaag atggtgcaaa ttggctgtac gggtcttttt
     1021 catgatgtta atgccgggtg ttgtaggaca cccggcacct ccgacaggtt aatggggctt
     1081 gagacgataa cgactactgc gtttacgtaa cgtcccggca gaaaagagct gttcgagcgc
     1141 atttctggct ttttccagcg gccagccaaa gtgggcagcc acttctcctg ccgtcattcc
     1201 ctgacgtact gatgttaaca gcgccaacaa ctcgtcagca gattccgcga cgataactgg
     1261 cgcgtcgggt tcctttacgg gttgcttagg ctggcggtta aagccagtaa ccagccactg
     1321 ggtgtcatcg tctggccgac cgatctcctt acggctgatg acacgcccgg tacgcatcgc
     1381 ggcagcacaa cctgcgtcca gcgccgcacg acacgccgcc agatcgcctt ccaccaccag
     1441 cgttaggcga ccagggtcaa gcacttcgtg actgagcaga cgaacgttag ctgctttgag
     1501 catggcatca gctgcatcta tcgcggcgac catgccgtcc acttccagca atcccagtgc
     1561 attgatcatc ggcaacctcc gttacgcacg ctggattgga ttacgcgcga tttccagcac
     1621 tgcatcggta aaggcgttac aggctgcttt acacgcggcc tggctaccgg ttaaaaatgc
     1681 tgccgagtag ttggtttcag acggtggcgg gacataggtc accagctgta cgtcggcaga
     1741 tttcagggct gcatcaatgc cgtaggtcgc ttccagcggc ggtgccacca gatacgccat
     1801 cggatcgcca agcgtgatcc cggcggttga tgagagataa gaaccggtac gcgaaactac
     1861 atgtgccagg aaggctgtgt tttctgcgtc attagcccac tggaaagcag cgccattttc
     1921 aatatgcgca accatcgcat ccagaccagc acgcacttcc gccgggtttg gcccaccaag
     1981 cataatcagc acttcaccgg cagtcggtga cgggccgtgt gcggctcccg catacagcga
     2041 gcggccatac accacttcca ccatcgcctg cttggtcgct tcgtcagccg caatgtaggt
     2101 gacgtcatct gaatcagcag aaatcagccc gaggctacga atatgcggcg gcaatttcag
     2161 ttcacgcgca aattcggcgt taacggaggc aatcacccgc atggcggtta ccgacggtcg
     2221 aatcaaatct aaagctggca tgatgcctcc ttatcgggtc atgttgatgc cggatgcttt
     2281 ctgctccagc atacgtttcg ccaaatccac aatgacggct gcggcttcaa ccggcggcgt
     2341 gccgccctgg tgaatgttag agatacaggt acggtcggcc tcaacggtgg ttgccatacg
     2401 cggcgagtaa acggcgtagc aggagaggct ttctgactgc cctaatcccg ggcgttcccc
     2461 caccagtagg atcactactt tcgcgccgag gatctcaccg atctgatctt caatcttcac
     2521 gcgaccataa cgaacaaaga atggtgtacc gactttcagc ccggcctgtt tcaggcccgc
     2581 catcagcggt ggcaggatct cttcgtagtt cacggtgatc gcatcagttg acaggccatc
     2641 agaaataacg acctgtacgt ccggattagc aacacactgt gctttcagcg cttcaacagc
     2701 ttctgcacac aggcggcggc ccatatccgg acgagtcagg tagagatttt tgtcgctaat
     2761 ttcagagcgg acttccagca agccctgcgc tttcacccac tcttccggta cttctttcag
     2821 tacggtatct ttcgaacgag agtgatcggc caggaaacgc agcaacgcct gggtacgcgg
     2881 acgcggaccg gcacgaccgg tacaaacgcg ggccacggtg ctgcgacgca gttctgttaa
     2941 tacgtctgcg cgatgcgggt tttccacacc aatccacgct tttgcttccg cagaacctaa
     3001 atccagcgcg cagctttccg aggtcactgg tgtcgtacag gtggtggtgg cgcactttgc
     3061 ttctgacggc gcgggggccg cttgtcccat tgacgccatc acgctgcgta caatttcttc
     3121 aatctgtttt tgatccatga tgtgttatct ccgcgtcatc agaagaacag tgacggatcg
     3181 cccgcccgtt tggtcaggcg accgtttgcc ataatgccca tgctttccag ccagcgttca
     3241 aactccggtg acgggcgcag gttgagtaac tgacgcacag tggcggtgtc gtggaaggcc
     3301 gtagtctggt agttgagcat gatgtcatca cccagcggca tccccatgat gtagttacag
     3361 cctgcggtgg cgagcaggat catcaggttt tcgttgaggt tctggtcagc gtcagcgtgg
     3421 ttggtgtagc agcagtcaca gcccatagag atgccgctca gcttgcccat aaagtgatct
     3481 tctaagcccg cgcggataat ctggcggtcg ttgtagagat actccggccc aataaaaccg
     3541 accacggtgt tgacgataaa cggatcgtaa tgacgcgcca gcccatagtt acgcgcttcc
     3601 atcgtcacct ggtcagcgcc gaagttagcg ccagcggaca gcgccgaacc ttgtccggtt
     3661 tcgaagtaga ggcagttttc cccggcgata cggttaaact ctgcacccac tgcgcgcgct
     3721 tcgtcgagca tcgccagctc cacgccaaac tctttcagcc ctttttcgct gccgcaaata
     3781 ctctggaaaa tcagcccgcc cggtgcgccg cgacgaatcg cttcgatctg ggtggtgacg
     3841 tgcgccagta cgcagccctg agttgggatg ttgaatttgt cgatcacgcc atagatggta
     3901 tccaacacgc ggcttaagtt ttccacgtcg tcagtcaccg ggttaacgcc gatcaccgca
     3961 tcgcccaccc cgaaggaaag cccttcgtag atttgcgcgg cgatactttg cacatcgtca
     4021 cgggtatcgt ttggctgcaa gcgggcgcta aaggtgcccg gaataccgat ggtggtattg
     4081 gcctttttga tcaccggcat tttcttcgcg ccgtagatca ggtccgcgtt ggagcaaatc
     4141 ttcgctaccg ccgctaccac ttccgaggtc agccctttgc gggtaaaggc aatgtcgtcc
     4201 acgctggttt catcgctcag cacatactca cgcagttcgc tgatgctcca gtttttaatc
     4261 tggttgtagg ccgtttcgtt aacatcgtcc tgaatcagcc gcgtcacgca gtcatcttca
     4321 taggcaatca ccggattatt gcggatgtcc gctacggtca tttccgacaa cacctgcttt
     4381 gccgccacgc gctcctgtga gcttgccgca gcaacgcccg ccagcacatc ccccgaacgc
     4441 agttcgttgg ctttagccag cacctctttt acatccttaa actgatatac attgccgaac
     4501 aatgtggtct ttagtttcat aagtcgttcc ctcaggaagg aaatgcgagt gatttcaccg
     4561 tcaccggcac aaccgatccg ccaaaaagag gcgtaccaat gtcgatatag tcccccgcgc
     4621 ggacaatcac ttcgtcaatg actgccaacg ggagttgttg tagctgtggg cgcaacaaca
     4681 tgcccagagc tttaccaaag tcctgcccgg ccaccaccag caggggatgc ggattcggaa
     4741 aacgcgcgac gaaatcgacc aacgcgttga tgaccgtcag taccgcggcg taacgcacag
     4801 gcagcgaggc gggaagcgcc agcacgtacg cgtcagtttt aggatcaaga tccagctgaa
     4861 tcagcgcctg ttgccaggca ctcaccagat ccttttcatc aattgggatc gccaccggca
     4921 agttgcgcag cggcagttgt acgccctcca gccagattgt gctgccagag agcgaaaggg
     4981 tatgtgcacc cgcgccaatc accgtggcgc gcacggtttg cgccggaaac tgcacgttca
     5041 tctcacgcag gcgcggatgg tcatgcagcg ccgttgccag aagcgggcca atatcggcaa
     5101 aacagaacgg gtcggcgggc tggtggcgat aacattcgcc aacgccgcca gaaagcgtaa
     5161 tgatttcggg cgtaacacct acgggcagca aaccggtttg catcaatgcc tgcgcgagcg
     5221 gcgaaagcgt tccgtcaatc acttcgacaa tcagctctgc catccgccgg gtaacctgca
     5281 ccagctgcgc gccggtcagc gaacgggctt cggtgcctgc accgaagcac tcatccacaa
     5341 tcatctgccc cggtttatga gcgtaaacca cacgcccctg gctgtcggtt tccagcaggc
     5401 gaccgccgac attgaggcag gcggtgccgc tgatttttcc ggcatcgaac agggcgtagt
     5461 tcgcggtgcc accgccgata tcgatattca ggacccgaca cagccgttgt tcagaaaggg
     5521 tttgcgcccc ggcaccgtga ccggcgatca cggattcgag atgcggtcca gcgctggcga
     5581 cgacaaaatc gcccagcgac tgggagagcg tcatcaccgc cgggcgagcg ttgcgggttt
     5641 tcgcgctttc accggtgatg atgatggcac cagaatcaac gctttccggc gcaatgcccg
     5701 cagcctgata ttgctcgagg attaaggttt tcagttccgc ttcttttaaa ccaccctgtt
     5761 tatcgacagg ggtaaagaac accgggcttt gccagctaat ttcgcgtttg atgaattcgt
     5821 agcgcggcac ctgcgacacc gccgcacggt taaccagctc caggtgggag aaaatcacct
     5881 gggtggtggt ggtgccgata tcgataccga cgctcagtag ctggcgagtg ttcacgattg
     5941 cgcctccgct tcggttttag ccgcgctcgc gtcttcttta ggtaccagca tcatcgccac
     6001 gccaatcgcc gtgacgccgc cgatcagctt gccgacaatc atcgggaaga tcatggcgtt
     6061 catgttggca gccgcgaagc ctaaatggtc gcccagggcg aaagcagcgg aaacggcgaa
     6121 ggcacagttg atgactttgc cgcgggtatc catctgcttc atcatgccaa acatcgggat
     6181 gttgttggca agcgttgcca ccatgccggc tgccgctata ttgttcatat tcagcacctt
     6241 accgacactc atcagcggtt tttcaaacca gcgagtcagc agcagcacca tcggatacgc
     6301 ccccagcagc acgcaggaga tagaaccgat aacttcaatg gcgcgcatca cttcaccggg
     6361 tttgtcgcct ggagccatga agatcggatc cagaccgggg atcagttccc aaccgagcag
     6421 gaatttcact accgcagcgg caagaccgag ggtgatcaat gcaacgagga atttggcgaa
     6481 gatctggaag ccgttgatca ttttttccgg gatgaatttc agccccagcg ccaccagcac
     6541 cgcaacgata agtaccggga tcatgttcat caggatcagc gcaaaggtga attccaccgg
     6601 ctgaccgttg atctgcacac cggagtacat agcaaccaga ccaccagcaa tacagccaat
     6661 cggaatggtc acaatgcccg ccagcacgcc gagcgccaga taacgacggt cagaaggttc
     6721 gataatgccg agcgccaccg gaatggaaaa cacaatcgtt ggccccatca tcgacccgag
     6781 aattaaccca gagtatagcc acgctgctac gtcgccgccc gccagctctt tggcgaggaa
     6841 gaagccgccc atatcgcacg ccagcagtgt tccggcgaac atcgatgggt tagcgccgag
     6901 catttcgtaa accgggataa tcaccggccc gagtacgtgg gccagtaccg gtgccagcgc
     6961 ggtcataccg accatagcca ggcccaaagc gcccattgcc ataaagcctt cttcgaactg
     7021 accgccggat ccttcgatac ttttaccgaa cttaccgagg aaacgagcag aaccgccgaa
     7081 ctgcgacagg atcctgtcta cggcagctat cagcataaag aacatcatga tgtacatgat
     7141 gatttcgtta atacccatcg cctttactcc ctgttagttg ttatttattg gcggatgcgg
     7201 cgtgaacgcc ttatccgccc tacatgtgca atcccgaagg ccagataaga cgcggcaagc
     7261 gtcgcatccg gcaattgcac cgcgccactg gcggatgcgg cgtgaacgcc ttatccgccc
     7321 tacatgtgca atcccgtagg ccggataaga cgcggcaagc gtcgcatccg gcatttattg
     7381 cgccgctgcg tacaggccga caatctgctc caggctggcg gtacgcgggt tactgcgcag
     7441 acaaatatct tccagcgcgg cctgcgccca tgcgccgtaa tgcgcagatg tcgcaccaac
     7501 atcgcccagt cgtttaccaa tcccaacttc cgctatcagc tcacttaccg cattaatagc
     7561 gtcacgatcg tcggattttt tagttcgcag cgcccgacca accaagctaa accgatcacg
     7621 acaaaccatc cggttaaatt ccatcaccgt tggcaacaac atggcgttcg ccagaccgtg
     7681 cggaatatgc agcgccgccc ctggctgatg cgccatcgcg tggcacaacc caagtccggc
     7741 actggaaaac gccattcccg ccatacatga agccagcaac atgctctcgc gcgcggcaag
     7801 gtcatgaccg tagcccaccg ctttcggcag cgatttgcca atcatcgcaa tggcaccaat
     7861 cgccagactg tcggtaaacg gtgtggcgtt cagcgcgctg tacgcttcaa tggcatgggt
     7921 taacgcatca atgccagtca tcgccgtgac atgcgacggc acaccttcgg tcaatgcggc
     7981 gtcgagaatc gccacatccg gcatcagcga ggcatgggct aacacctgct tgcgcccgct
     8041 caccgcgtcg ataatcaccg ttacgttggt ggtttcagag ccggttccgg cggtcgttgg
     8101 aatggcaatc aacggcaggc gcggttgcag aacgctggtt tctgacattt ctgccagcgt
     8161 gctatcgggg ttcgtcacca gcaacgccac ggctttcgcc gcatccagca ccgagccgcc
     8221 gccaaacgcg atcaccccgt cacaacctga ttcacgcaac tgcgccacgg ctgcacacac
     8281 gtcggtgatg cacggttcgc ccaccggaca tggccagagc gtcatggcga tacctttaac
     8341 ggccaggcta cgcgtcagcc cggcggtcat ccccgcctga tgcaaaaagc tgtctgccat
     8401 cacgaacaga tgtttcagcc cacgcgtttg cgcttgctgc ccacaactgc tcaccgcgcc
     8461 cggaccgcaa agcgtcaccg gtggaacgct aaatgttttc acccgctgca gattcagggt
     8521 atcgaatgcc tgaaaaagcg ccgtttgcaa ttcactttgc atagatcccc tccgttttct
     8581 ctcgcccact actggcgatc gccagcgggg tcataaacaa actgtgctgc ggtaaatgca
     8641 cctgtaacgc cgggaattgt ttgcgaaaca gttccgctac gcccggttgc atacaggaac
     8701 cgcccgccag ccataaatcc gtaatgccct gcccttcaat atgccgggcc acaatgtccg
     8761 ccattttttc gtacaccggt ttcaccgcgg gccaaatctc gtcaccgtga ccgcgtttgt
     8821 actgctctgc ctcttccagc gaaatacggc gatttccggc gagagtcaga gagatgtgat
     8881 gtccgccggt ggcttcatcc gccgagtacg tcactttgcc ctttttcacg atggcaatgc
     8941 cggtagtgcc gccgccgata tccactacac cggcgttgtc cagttgcagc agatccgcaa
     9001 ctgcggttgg ctcatccagc acatggctca cttccagtcc agcagactcc agcacgttga
     9061 tggaaatacg cgggtccgta cctggaggaa aagaggtcgc cgcatggcta aaacggcgac
     9121 cgaattgctg ctcgagggtg tcgagatggc gacgaacaat ggtgaccgca ccgaagaaat
     9181 cccagacgat gccgtcgcgg accacatcag cccagtcgag gcataccgcc accggctgac
     9241 cgtcgcggtc gacaaccatc gacaccacat cgcaggtgcc cagatccacc cccagccaca
     9301 gcggagattc cgtcgcggcg ggcgtctggt tacacagcgt tgccgccgtt tgcagacgtg
     9361 gggtgagcca ttgttcgtcg tgcgccatct gttactcctt aaacaatgcg aaacgcatcg
     9421 actaatacac agcgacgcag acggacaaac gtccgcgcgc tggttacccc ttcaccggtt
     9481 ggcgtggtga tggtcatagt ggtccagcct tccccgccca gccccagccc ggcaatgcac
     9541 ggtccgttct taacgaaaat gctggtatca atggcattcg ccatctggtt catgttctcg
     9601 atattgcgcg agtgcattgc cgccgtgtgg tggcaaccgc cttccagttt caccgccagc
     9661 gcaatggcat ccgccacgtt ggcgacgcgc acgacgggca gcaccggcat catcagttca
     9721 gtcacagcaa acggatgttc tgcggtggtt tctacaaaca gcagtcgcgt ttgttccgga
     9781 actttaaggc cgattgccgc cgcgattttc gccgcatcgc gaccaaccca gtcacggctg
     9841 acggtgcctt ttccgcgctc gtcgatattt ttcagcaaca ccgattgcag ttgctgcgcc
     9901 tgctctgcgg tcagtttcac cgcatgctgg ccttccatca gacgcatcag ttcatcggct
     9961 acgctatcaa caacaatcag taccttttcg tcggcacaaa tgatgttgtt atcgaaagaa
    10021 gcacctttga cgatggactg agctgcacgg gcgaggtcgg cggtttcatc caccactacc
    10081 ggcgggttac cggcacctgc cgcaatcagg cgtttattgg tgtgtttgcg cgccgcttcc
    10141 actaccgctt cgccgccggt caccaccagc aggccgatac ccggaaactt gaacaagcgt
    10201 tgcgcggttt cgatatccgg atttgctacg gtaaccagta agttttccgg cccgcctgcg
    10261 gcaacaatcg cctggttgag cagcgtaatc gcccgctggg agactttttt cgccgccgga
    10321 tgcggggcaa aaatgacgct gttgcccgcg gcaatcaggc tgatggcgtt attaatgacg
    10381 gttgccgccg ggttagtgga aggcgtcacc gaagccacta cgccccaggg cgcgttttca
    10441 attaaagtca ggccgttgtc gccagtcagc acctgtggag agaggcactc aacgcctggt
    10501 gtgccgcgcg cctgagcgac gtttttggca aatttatctt caacgcgccc catgccggtt
    10561 tcactgacgg caagttccgc taaatctctg gcgtgttttt cgcctgcttc acgaatggca
    10621 gcaatggcta actggcgcat tgccacgctt tttaaccctt gctgggcgac tttggctgcc
    10681 gcaacggcgt catccaggga cgcgaaaacg cccatctcat gaacggcggc gggcggcgtg
    10741 tcactgcttt gcattttcag cagtaccgct ttcaccacct gttcaatatc ctgttgattc
    10801 atgatgttct gccttatttg tggaaaatga cctgaccgcc agacaccacc tcatcgacga
    10861 tgccaatcac gcacagatcg accggtgacg tttcgctttt atgcgcctgg cgggcggaac
    10921 tgccactcac cagcaacacc cactccccgg ttcccgcgcc aatattgtcg atggcgacgg
    10981 cgcattgccc gtcaggatta ccttgtggat caatcatttc caccatcagc aatttgtcat
    11041 gcgccagtcc gtgatggcgt acggtacaaa caatttgtcc agtgacgact gccagtttca
    11101 tacccgcctc cgtggcgtat ttcaggtaaa agctccccct accctccgca gaaggtaaaa
    11161 tgaaaaagga gagagcgtga cgcccgaatc gacgtcacac agggtgatta caggttgctg
    11221 ctatcgcctt tcaggccgat cgggaagact tcttccaggt cgccgtgcgg acgcgggata
    11281 acgtgtacag ataccagctc gccgatacgc tgtgccgctg cggcacctgc atcggtcgcg
    11341 gctttacagg ctgcaacatc accacgaacc atggcagtac acaggccgcc gccaatctgc
    11401 ttaacaccaa ccagcttgac gcgcgcagct ttaaccatcg cgtcagaagc ctcaatcagt
    11461 gcaaccaggc cccgggtttc gatcattcct aatgcttcca ttgtgctttc ctctttatca
    11521 gggtccagaa cgggaccgtt cattcaacca gtgtttgtaa actgctttcg cggttcactt
    11581 ctgtctgacg ctgcacagct gccaccagcg ccagttcgat aatttcctgc acgctacaac
    11641 cacgagagag atcgtgcatc ggcgcggcaa gtccttgtat caatggcccg acggcacgat
    11701 atccgccaag tcgttgtgcg attttgtagc caatatttcc ggcttccagc gacggaaaaa
    11761 ccatcacatt agctttgccc tgtaacgggc tggcaggcgc tttttgcgcc gccacttccg
    11821 gcacgaaggc ggcgtcaaac tgcaactcgc catcgacaac cagctttggt gcgcgctcac
    11881 ggacgatttc tgtcgcctgc tggacgttgg caacgcaggg gtgacgggca ctaccgttgc
    11941 tggaaaacga caacatcgcc acgcgcggtt cttctccggt gatggcgcgc caggtttcgg
    12001 cgctggcaag cgcgatatcc gccagttgcg ccgccgtcgg ctgtggcact acgctgcaat
    12061 cggcaaagcc caatgcgggg ccactgtact gtggcagcat caggaaaatg gaggagagcg
    12121 ttttacagcc tggctgtaag ccgataatgc gtaatccggc acgcagcaca ttcgccgtgg
    12181 aagagagatt gcccgcgata cagacatccg ctgtcccggc gctgaccatt gcggcagcga
    12241 acatcagcgg atcggtgagt ttatccagcg catccggcgg cgttttttcg cccgcacggg
    12301 ccagccagcg gagagcaaat tcttcccgca ttgcgaggtt gccatgcgga tcaatcacct
    12361 gtagcccgtc catcgccacg ccgtgactga gcgcaaactg acgaagttca aacggattgg
    12421 cgaccagaat gggcgttgcc agaccttgtt gatgtaaata ttgcgccgct ttcagcacac
    12481 gttgatctaa cgcatccgga aaaactactc tggcgggcgc tcgcaacgcc agtttacgac
    12541 aacgttcaat aatcattgcg tttcccccag tcgttgttgg atctgcttca ccgttagcga
    12601 ctgcttcgtt acgctcagga tcatcatcac gtaaaccgtg ctggaaaggc ggttcagcgc
    12661 ctgcaagatg tccgggcgta atacttcaaa gctgcgggtg ataaacacct gcgccgctac
    12721 agtttcggtt tcccgcactt tggtgcgtag cagattgagc aacgccgcat cacgcccgtg
    12781 gctggcttcc ggcaccagat gatcgtggtc gagatagcgc agcggctggt gagaaagccg
    12841 atgcagatct tcgtcagaaa gcccgacaat cgcttgttcg cccagcggtt cccccagcgc
    12901 atcggcgcgc ataatgttgc caagacgcga acggatatcc gccagccacg gctgccacgg
    12961 ttccgccagt tcaatttgca gccacactgc cagcgcaatg gtgctgtcga gaacagcgcg
    13021 aaaagccagg cgcggatcgc ttttggcaac cattttttcc gccgacaggt gagtcagcgt
    13081 atccggcttt tttgccaccg gctggcggca cagttcgcag cacgcctgcg gatgttcatc
    13141 gctactggtt agcccgtgaa cgggctgcgg ctgctgctgt tcatcgtcaa caaacaggcg
    13201 gccctgctcg tcaataaact tgatgcgcag atggcggctt tccagtaact cccgggcaga
    13261 gggcgtcagg cgactgtccg cagggagatg gatctctgcg ccttcgctga gcgtatgatt
    13321 cgctcttagc catgcttcgg tgatgaaatc tttcataagg attgccagtt agccggccag
    13381 gcgacataca ggaatttcac gctggatgtc gtaccaaatt cgatgctgga gcctttcggg
    13441 ataaacatca catcgcccgc tttggcgatc atagtttcgc cttcgtggcg aacatgcagc
    13501 tccccttcca gcaccatgtc gatttcgtcg tagttcaggg tccacggaaa gaaggcgttc
    13561 tcccactgca tgaaaccagc ggccatactg ctgccgtcgt ctccggtcac gagatcagtc
    13621 aagcctacgc agtgcggttc cgcgccatcg aaacgaccaa acttgacgct gctgccatca
    13681 ataactttga tgccgccttt gccggtcacc gatttaaagc tcggctgcag tgcaccctgc
    13741 tccagcgact gtttttcctt catcactttt tccatcagct gcgcgaccag gctttcggta
    13801 aactgacctt ccggcagttg ggcgataatg gtttcacgga tgcgctggct ttcggtttta
    13861 tcggctgatg cgctggcggg aacagacgcc gttaccggga tcgattcatc acattcggtg
    13921 atggtaaaac ccaacagatc agccacttca cgagcttccg gggtaataat gctggcgcgc
    13981 agaaccacgg acattgccag ttcgccgcgc gcgtgtgctt cacgaatatc attcgctgtg
    14041 atgagttttt tcacctgctt cctcctgttt ggtgagagat gccagataat ccaccagttg
    14101 ctgcacgctt tgcgggtcgt ggctgtttag ctcgaaaatc ggctcttcga agcccgtctc
    14161 aagcaataac tttcgcgttg cggcgacgtc agcatctggc atgtccgttt tactgatgac
    14221 ggcgatttgc cgtttgctga cgccaatatc cagcaacccg gcaggcaacc gactttccgg
    14281 gtcattcgcg ccgtggacat agatcagcat gtccacatcc tgtagcgtgg taattaatgc
    14341 gtgataccag cggggatggc taaaatattc acccggcgta tcaatatcgc cattatcatt
    14401 aaattccacg gcctgcgttt ttctggcgag ggtataattc ccctgcaacg cattaaataa
    14461 cgttgttttt cctgccccga cggtacccac aaacgcaatt cgtttcatga atgcctcata
    14521 ttaacttttg gtcatttcac aaagcgtata atttaataag cgtcctaaac cactgacggt
    14581 ttgtgataac gcttcttcta ctgcgcccac cgaaccataa atcaccagcg cgccgctaaa
    14641 acggtcgaga aaaccgatat gcacatcggc ggctttcaac gctaaatcac cggcgatcat
    14701 cgccgtttct ccgggagtta gcgtcataat gccaattgcg cccgcatccg gaacgccgat
    14761 tttcttcgcc agttcctcgc cgggatgcgc gatgagatgc gccagcgtga cctgtttgcc
    14821 gggcacaaat tcctgaatga tgcgttcttt atccatgagt cgccgcctgt gaaaaacttt
    14881 cgtgtatcgt gacaattttt cgtcagagta aataacaaaa ttcggcaagc gcgtttaaaa
    14941 ggtgaggtag atcactaaga atggagagat atttactgtt atgaataaac tttcatatgc
    15001 gacgcatgaa tataaataaa ataatatcga gatattttat atactgattt ttttattatt
    15061 cgtaaactca gtaataagct ttactaatat attgcagcta aactgcttac cctgaatatt
    15121 cagggtaagc gtgagagtta aaaaaaaatt acagcggttg ggtttgcgct tcaaccacgg
    15181 ccagcgccac catattgacg atacgacgta ccgatgcgat cggcgttaac acgtgaaccg
    15241 gtttcgccac acccatcagc accggaccga cagtcacgcc ttccgagctg gaaacgcgca
    15301 gtaagttgta actaatgcgg gcagcttcca tgttcggcat caccagaata ttagcggaac
    15361 ctttcaaagg gctgtccggc atacggtcat tccgaatcgc ttccaccagc gctgcatcgc
    15421 cgtgcatttc accatcaatc atcagttctg gtgcacgttc cctgaccagt tccagcgcct
    15481 gacgcatttt gctcgacgac gggcagtcag aagaaccaaa gttggagtgc gacaacagag
    15541 ccacacgcgg ctcaatacca aaacgacgaa cagtttctgc cgccatcaag gtgatctccg
    15601 ccagctcttc tgcatcaggc tcatcattga cgtaggtatc ggcaataaag gtgttaccac
    15661 tcggcaacag cagcgcgttc attgcacctg cggtgtgaac gccatcgcga taaccaaaga
    15721 catttttcac cacgctaaag tgttcatgat aatcgcccac cgtaccgcaa atcattgcat
    15781 cggcttcgcc acgctgaacc atgatcgcgc cgatcactgt cgggttactg atcagcgcac
    15841 gctgcgcctg ctcctgagtg acaccgcgac gcttcatgat ctggaagtat tcggtccagt
    15901 actctttaaa gcgcggatcg gattcgttat tgacgatctc aaaatcaacg cccgccttga
    15961 tctgcaagcc cagtttctga atgcgcattt cgatcacgtt cggacgacca ataaggatcg
    16021 gtttcgccag tcccagcgtt accagttcct gagtggcatg cagaacgcgc gcctcttccc
    16081 cttccggcag aactacgcgc ttcggcgctt tgcgagcctg ggagaaaatc ggcttcataa
    16141 acaggttagt tttgtaaacg aactccgtca gcttgtcgat gtagacgtcg aaatcagcaa
    16201 tcggacgcgt cgccacgccc gactccatcg ccgctttcgc caccgcagga gcgatcttaa
    16261 cgatcaagcg cggatcaaac ggttttggaa tgatgtattc cggaccaaag ctcagatcct
    16321 gatcgccata cgctgaagcc accacttcac tctgttccgc atgggccagt tctgcaatcg
    16381 cgcgtaccgc cgccagtttc atctcttcgt tgatggcggt tgcgccaacg tccagcgcgc
    16441 cacggaagat aaacgggaag caaaggacgt tgttcacctg gttcgggtag tcggaacggc
    16501 cggtacagat gatggcatcc ggacgcactt ctttcgccag cggcggcaga atttccggtt
    16561 ccgggttcgc cagcgccagg atcattggcg cacgggccat tttcttcacc atttcctggg
    16621 tcagcacttt cgggccggaa cagccgagga aaatatccgc gccttcaatc acatcatcga
    16681 gggtacgttt accgtcatcc accaccgcgt atgcggcttt ggtttccgcc atgtttggct
    16741 cacggccccg atagataacg ccttttgaat cgcaaaccac gatgttatgt ttttgcagac
    16801 ccagcgctac cagcaggttc atacaggcga ttgctgcggc acccgcgcca gaaaccacca
    16861 tccgcacgtc ggagatgttt ttctccacca cgcgcaaacc gttgaggatg gcggcagtgc
    16921 tgataattgc cgtgccgtgc tgatcgtcgt ggaataccgg aatattcatc cgctcgcgca
    16981 gtttctgttc aatatagaaa cattctggcg ctttaatgtc ttcgaggttg atgccgccaa
    17041 aggtcggttc gagcgcagcg acgacttcaa taaatttgtc cgggtcgagt tcatcaactt
    17101 ctatgtcaaa tacatcaatc ccggcgaatt tcttaaacag aacgcccttg ccttccatca
    17161 ccggtttgcc tgccagcgcg ccaatgttgc ctaaccccag caccgccgta ccgttagaga
    17221 tcaccgctac caggttaccg cgcgcggtat atttgtaggc ttttaacggg tctttttcga
    17281 tttcaagaca aggtgcggca acgcctggtg agtaggccag cgccagatcg cgctgcgttg
    17341 ccagaggctt ggttggagaa acctggattt tccctggaac tggaaattca tggaaatcaa
    17401 gtgcactttg ttttaactgg tcatccattt ggttgttcct ttcacgtaac gttcacaaat
    17461 aaagtgtgtg ggcaacagcc cctgcccaca acgtggcgca cattattacc ttgccggagt
    17521 ctacagactt tgagcaagtc caaactctca ccattaatat aatgttttgg taataatcct
    17581 ataacactga tgttacctgc ttaatccagc aataccatgc ctgtctgcta tgcttttttg
    17641 atgcgtttag cgaaatttct cagaagtgtg aattaacgca ctcatctaac actttacttt
    17701 tcaaggagta tttcctatga acgagttaga cggcatcaaa cagttcacca ctgtcgtggc
    17761 agacagcggc gatattgagt ccattcgcca ttatcatccc caggatgcca ccaccaatcc
    17821 ttcgctgtta ctcaaggctg ccggattatc acaatatgag catttaatag acgatgctat
    17881 cgcctggggt aaaaaaaatg gcaagaccca ggagcaacag gtggtcgcag cgtgtgacaa
    17941 actggcggtc aatttcggtg ctgaaattct caaaatcgta cccggtcgcg tgtcaacaga
    18001 agttgatgca cgcctctctt ttgataaaga aaagagtatt gagaaggcgc gccatctggt
    18061 ggacttgtat cagcaacaag gcgttgagaa atcacgcatt ctgatcaagc tggcttcgac
    18121 ctgggaagga attcgcgcgg cagaagagct ggaaaaagaa ggtattaact gcaacctgac
    18181 gctgctgttt tcttttgcac aggcgcgggc ctgtgcagaa gcaggcgttt ttctgatttc
    18241 gccgtttgtc gggcgtattt atgactggta tcaggcacgc aagccgatgg acccgtatgt
    18301 ggtggaagaa gatccaggcg ttaaatcagt acgcaatatc tacgactact ataagcaaca
    18361 ccactatgaa accattgtga tgggcgcgag cttccgtcgc accgaacaaa tcctcgcctt
    18421 aaccggctgc gaccgactga ctatcgcacc gaatttgctg aaggagctac aggaaaaagt
    18481 ttcgccagtg gtacgtaaat taatcccacc ttctcagacg ttcccacgcc cagctcccat
    18541 gagcgaagcg gaattccgtt gggagcacaa tcaggatgcg atggcggtag aaaaactgtc
    18601 tgaaggcatt cgtctgttcg ccgttgatca acgcaaactg gaagatcttc ttgccgccaa
    18661 actataaacc agccacggag tgttatatgt cccgaaaaga ccttgccaat gcgattcgcg
    18721 cactcagtat ggatgcggta caaaaagcca attctggtca tcccggcgcg ccgatgggca
    18781 tggctgatat tgccgaagtg ctgtggaacg attttcttaa acataatcct accgacccaa
    18841 cctggtatga tcgcgaccgc tttattcttt ccaacggtca cgcgtcgatg ctgctctaca
    18901 gtttgctgca tctgaccggt tacgacctgc cactggaaga actgaagaac ttccgtcagt
    18961 tgcattcgaa aacccctggt cacccggaaa tcggctatac gccaggtgtt gaaaccacca
    19021 ccggtcctct tggacaaggt ttggcgaacg ccgtcgggct ggcgatagca gagcgtacgc
    19081 tggcggcgca gtttaatcag ccggatcatg aaattgtcga tcacttcacc tatgtgttta
    19141 tgggcgacgg ctgcctgatg gaaggtatct ctcacgaagt ctgttcgctg gcgggcacgc
    19201 tggggctggg caagctgatt ggtttttacg atcacaacgg tatttccatc gacggtgaaa
    19261 ccgaaggctg gtttaccgac gatacggcaa aacgttttga agcctatcac tggcatgtga
    19321 tccacgagat cgacggtcac gatccgcagg cggtgaagga agcgatcctt gaagcgcaaa
    19381 gcgtgaaaga taagccgtcg ctgattatct gccgtacggt gattggcttt ggatcgccga
    19441 ataaagcagg taaagaagag gcgcacggcg caccgctggg tgaagaagaa gtggcattgg
    19501 cacggcaaaa actgggctgg caccatccgc catttgagat ccctaaagag atttatcacg
    19561 cctgggatgc ccgcgaaaaa ggcgaaaaag cgcagcagag ctggaatgag aagtatgcag
    19621 cctacaaaaa ggcccatccg caactggcag aagagtttac ccgtcggatg agcggtggtt
    19681 taccgaagga ctgggagaaa acgactcaga aatatatcaa tgaattacag gcaaatccgg
    19741 cgaaaatcgc tacccgtaag gcttcgcaaa atacgcttaa cgcttacggg ccaatgcttc
    19801 ctgaattgct cggcggttcg gcggatctgg ctcccagcaa cctcaccatc tggaaaggtt
    19861 ctgtgtcgct gaaggaggat ccggcgggca actacattca ctacggggtg cgggaatttg
    19921 gcatgaccgc tatcgccaat ggcattgcgc accacggcgg cttcgtgccg tataccgcga
    19981 cgttcctgat gtttgttgaa tacgctcgta acgccgcgcg gatggcggca ctgatgaaag
    20041 cgcggcagat tatggtttat acccacgact caattggctt aggggaagat ggtccgaccc
    20101 accaggccgt tgagcaactg gccagtctgc gcttaacgcc aaatttcagc acctggcgac
    20161 cgtgcgatca ggtggaagcg gcggttggct ggaagctggc ggttgagcgc cacaacggac
    20221 cgacggcact gatcctctcc aggcagaatc tggcccaggt ggaacgtacg ccggatcagg
    20281 ttaaagagat tgctcgtggc ggttatgtac tgaaagacag cggcggtaag ccagatatta
    20341 ttttgattgc caccggttca gagatggaaa tcaccctgca agcggcagag aaattagcag
    20401 gagaaggtcg caatgtacgc gtagtttcgc tgccctcgac cgatattttc gacgcccagg
    20461 atgaggaata tcgggagtcg gtgttgcctt ctaacgttgc ggctcgcgtg gcggtggaag
    20521 caggcatcgc cgattactgg tacaaatatg ttggtctgaa aggggcaatt gtcgggatga
    20581 ccggctatgg ggaatctgct ccggcagata agctgttccc gttctttggc tttaccgccg
    20641 agaatattgt ggcaaaagcg cataaggtgc tgggagtgaa aggtgcctga tggtgatcgc
    20701 cggatgctat atgttcccct caccctaacc ctctccccag aggggcgagg ggacggtccg
    20761 tgcgcttttt aggggcgcat ctgggatttt gtttaacggg tgatccacaa cgtgggccag
    20821 gcgtctggcc catgccagtt atcgcaggtg ggttctgcgg cgtaacgcac caggcgaaaa
    20881 cgctgaccgt caaagcgcca gcgcgcctga atgccacaat cgcttaatcc gcgccctttc
    20941 gctaaggtca ccagttcacg cgatttttca tcgaatgttg cgttcatcag ttccagttca
    21001 ttcgtctcct gaccgctgtt gaacggcaaa cgtaaccgaa ccgggcgcga ggccagtggc
    21061 tttttacgcg acacaatcca tgccaaatca atggtgttat aggcccctgc ctcacagcta
    21121 atcatcatca gcgctttatc atcggtcagc gcagtgacat tcacctctcg acgcaatgga
    21181 tcaagcgagc agcgcagacc attcatccgc cagtttccat aatccagcaa atcgttgcgt
    21241 tcttcgagtg agagtggcgt cggcgttggg ttaaccaccg cgacctcttt cagcgcaggc
    21301 gcgggcggta cgctgagcgg cggttcatcc cctttcttta tccacgcggt ttcactgcca
    21361 acgcgctttt gctgagcatc tataaacaac aacgccgctt ttaagccact cagagaaata
    21421 gtctgattgc cgtcgcgtaa ggtgatcgcc ctcccttcct gaatcatctg caaaaacgcg
    21481 gtgatggttg ccgtatcatc ggttactaat aaccatggtg aaatccgcca cttgtcacca
    21541 cttaacgcca acggctcacc atctaacagc agccgtggcg ctatctcccc ttcagacgca
    21601 tccggcgact tcaacccgcc gcgctcaata cgtaaaacgg catcggtatg cgccccggcg
    21661 ctgcggctca gggtcatcac cagtccgtta tgatcgcccg tattacgcgc cacgcagaag
    21721 ttttggttat tacaggtgac ctgccagtcg gaaaacgccc gttgcgctgg tgccgcccac
    21781 accaaagacg tcggcaacaa agcgaaaaaa aagagaagga aaatgcgata gcgcatggac
    21841 ggtacgaccc cagaagttca aacaaacagc acaagtcgta tcttcgttgc gcaggtagcg
    21901 cggctcaatc ggatttatcg gattgagcca ataataaaca acgaaatatt attgttattt
    21961 ttcagtccat taaatgtgac atttgcaaat agttaagcaa taacaccgtc ttaccgtcac
    22021 gtatctcgcc agttttgatc atctccagcg ccttgttgaa cggcagctcg agcacttcaa
    22081 tatcttcatc ttcgacacca ccgccggcgt tagcgcgctg gctgtcactg tattcggcga
    22141 taaagaagtg gattagctca gtcacaccgc ctggcgacat atacagttca aataatttgc
    22201 gcacttcgcc aacttcataa cccgtctctt caatcgcttc tttacgaata cacacttccg
    22261 gttcgtcgtt atccagcagc ccggcgcagg tttcaatcag ttgcccgctt tcattgccat
    22321 taacccaggt agcgacgcgg aactggcgaa tcagaaccac ggtctttttc ttcgcgttgt
    22381 acaggaggat cgttgcacca ttaccgcgat cgtaaacttc acgtttatgg cggataactt
    22441 cgccatcttt gcgcgtgaga tcgtaagtaa tgttgtgcag ggtgaaatag ttatcggaga
    22501 gaattttgtc tttaatgagg gtgatttgtt gcgtcatacc gactccacag cgcgaaatga
    22561 acaattatct tacgctgtga agccgggttt gtctgcgcag gctatcagtg agatttgact
    22621 gattttaccc ccagccagtc aataatcccc tgtgccgcat gacgtccttc tgccattgcg
    22681 gtaaccacca gatccgcacc acgcacggcg tcaccaccgg cgaagatttt cggattggtg
    22741 gtctggtaac ggtactggct ttccacatcc gcgatgatgc ggccccattt gtctaccgtt
    22801 acaccgtgcg actccagcca cggcatcccg tgcggattga agccaaacgc cataatcacc
    22861 gcatcggctg gcatgacaaa ttcactgcct tccaccggca ctggacgccg acgcccctgg
    22921 gcatccggct cgccaagacg cgtgcgcagg aaacgaatcc cgcagacgtg accttgttca
    22981 ttcagttcaa gcgccaccgg ctggacgtta aattcgaagt tggccccctc ttcacgggcg
    23041 ttcttcacct ctttcttcga gcctggcatg ttagcttcat cacgacgata agcgcaggtg
    23101 acgttactcg cgccgtggcg cagcgcagta cgcacacagt ccatcgcggt gtcgccgccg
    23161 cccagtacca cgacgttaag tccggcagta ttgataaacg gctcttccgg tagctcttcg
    23221 agtcccatca cctgtttagt attggcgatg aggaacggca gcgcgtcata aacgcccggc
    23281 gcatcttcat tgggtaaacc cgctttcatg gagcggtaag tgcctacgcc aacgaagacc
    23341 gcgtcgtatt gttccaaaag cgaattcaaa gagacatctt tacccacttc acaattgagt
    23401 tcgaagtgaa tccccatcgc gctgaagatt tcccgacggc gtgccagcag ggatttatcc
    23461 agtttgaaag aaggaatgcc gaaagtgagc aagccaccga tttctggatg gcgatcgtac
    23521 accgtcacct ctacgccatt gcgggtcaaa acatccgcac aggccagccc tgccggaccc
    23581 gcaccgataa tcgccacccg cttgtccact ttggtgacat ggcttaagtc aggacgccaa
    23641 cctttcgcca acgcctgatc tgaaatgtag cgttcaatgt taccgatagt tactgcgccg
    23701 tgctcatcgc gaatagtaca ggcaccttca cacaaacggt cttgcgggca aacgcgtccg
    23761 gtaatttccg gcagggtgtt ggtctggtga gaaagctcga cggcggcgtc gatgtttccg
    23821 gctttcacca gttcaatcca ctgcggtata tggttatgca gcgggcaggt ccattcacaa
    23881 acgctgtgct cgccgcactt aaggcagcgc gaggcttccc gttgtacctg atcggcacgg
    23941 aatggcagat aaatttcatc aaaaccggtt ttgcgcgctt caatcgccag tttatctggc
    24001 tcgccacgcg cgggcgttgc ctgcatttgc tcgactttac tcattaccgg catttcttgc
    24061 gccgcggtac tggcatgcca cggttgatgt tcctgacgtg cggtacgcaa gcggcgggat
    24121 ttcgccatgc ctgagagtgc ggcgtcagtg accagttgca gcgcgtccgc tgggcaattc
    24181 tcgacacaag caggaccgtt ttcgcgccct gcacaaaggt cgcatttatg cgccgtggct
    24241 tttacttttc ctgccgcgac gggggtcagg acgatttgca tcgtaccaaa tggacaggcc
    24301 accacgcagg atttacagcc aatacacttt tgctgattga cctgaatgct gtcatcaacg
    24361 tggctgattg cgccattagg gcagctacgg gcgcagggcg catcttcaca atggtgacag
    24421 gtcacggcac tacgttgctg ctgatgtttg ataaccgtaa ttcggggatg aaaatggtgt
    24481 tggctcagga catgttgctc atcattgtga gccatgacac aggcgatttc acaagcatga
    24541 caacccagac attgctgact gttggccata ataaaacgat tcataacgac cttctttttt
    24601 ggttgtaaaa accttattct ttatatgagt gttgttatta cccgacttac aggggaatcg
    24661 gcaatgttca tgtgcccaga ataagtaact atttcgctat aaactgtggc agatcaaata
    24721 atcccatcac tgactaaatt gcgtttcagc gaattggaac attaatgatt ttttgtggag
    24781 aagacgcgtg tgattgttaa acgacccgtc tcggccagtc tggcccgggc ctttttttac
    24841 attgtgctgc tgtcgattct ttccacgggt atcgctctgc taacgctggc gagcagttta
    24901 cgcgacgctg aggctatcaa tattgccgga tcgctgcgta tgcagagtta ccgcctgggc
    24961 tacgacttgc aaagtggcag tccacaactc aatgcacatc gccagttatt tcagcaggca
    25021 ctgcattcac cggtattaac caatctcaac gtctggtatg tgccagaggc agtaaaaacc
    25081 cgctatgcgc atctgaatgc caactggctg gagatgaata atcggctcag caagggcgac
    25141 ttgccgtggt atcaggccaa tattaataat tatgttaatc agatagacct gttcgtgctg
    25201 gctttacagc actacgccga acgcaaaatg ctgctggtgg tggcgatctc cctggctggc
    25261 ggcatcggta ttttcacgct ggtctttttt actttgcgcc gcatacgcca tcaggtggtt
    25321 gccccgctga atcagctggt taccgccagt cagcgtattg aacacgggca gttcgactcg
    25381 ccgccgctgg ataccagcct gccgaatgag cttggcctgc ttgcaaaaac ctttaaccag
    25441 atgtcgagcg agctgcataa attgtaccgt tcgctggagg cgtcagtaga agaaaagacc
    25501 cgcgatctcc acgaggccaa gcgtcgtctg gaggtgttgt atcagtgttc gcaggcgcta
    25561 aacaccagcc agattgatgt gcattgtttc cgccatattt tgcagattgt tcgcgacaat
    25621 gaagcggctg aatatctgga gttaaatgtc ggtgacaact ggcggattag cgaagggcag
    25681 ccaaacccgg aattgccgat gcagatttta ccggtgacaa tgcaagagac ggtttacggc
    25741 gaactgcact ggcaaaatag tcacgtttca tcatcagaac cgctgcttaa cagtgtttcg
    25801 tcgatgctgg gacgcggttt gtactttaat caggcgcaga agcattttca gcagttattg
    25861 ttgatggaag aacgtgcgac catcgcccgc gaattgcacg actcgctggc tcaggtactt
    25921 tcttacttac gtatccagtt gacgttactg aagcgttcga taccggaaga taacgccacc
    25981 gcacaaagta tcatggccga tttctcccag gcgctgaatg atgcttatcg gcagttacgc
    26041 gagctgttaa ctactttccg cctgacgctg cagcaggcgg atctcccctc cgcgttgagg
    26101 gaaatgctgg atacgttaca aaatcaaacc agcgccaaac tgaccctcga ctgccgtctg
    26161 ccaaccctgg cgctggatgc gcaaatgcag gtgcatttgt tgcaaattat tcgcgaagcg
    26221 gtgctgaatg cgatgaagca cgccaacgcc agcgaaatcg ccgttagctg cgtcaccgcg
    26281 ccggatggca atcacacagt ctatattcgc gacaacggga ttggcatcgg tgaaccgaaa
    26341 gaacccgaag gccattatgg tctgaatatc atgcgcgaac gcgcggaaag attgggtgga
    26401 acgctgactt tttcacaacc ttccggcggc ggcacgttag tgagtattag ctttcgctct
    26461 gcggagggtg aggaaagtca gctgatgtaa tgcctcttat tgaccaaaga atagtggcac
    26521 ttaaggttca gtataaaagg gcatgataat ttacattaac tccttttttt ctccacgatt
    26581 ggctcgtacc ttgccgctac agtgaagcaa gtcaagccta caacgatacg cagaaacacg
    26641 aggtcctctt ttaatggcga atttctttat tgatcgcccc atttttgcct gggtgctggc
    26701 aatcctgttg tgtctgacag gtaccctggc gattttttca ttgcccgttg aacaataccc
    26761 cgatctcgcg ccaccgaatg tgcgagtgac cgctaactat cccggcgcat cggcccagac
    26821 gctggaaaac accgtgaccc aggttatcga gcaaaatatg accggcctcg ataatctcat
    26881 gtatatgtcg tctcagagca gtggcaccgg ccaggcatct gtcactttaa gttttaaagc
    26941 aggcaccgat ccggacgaag ccgtgcagca agtacaaaac cagctgcaat cagccatgcg
    27001 aaagttaccg caggcggtgc aaaatcaggg cgtgacggtg cgtaaaaccg gcgataccaa
    27061 tattctgacc attgccttcg tctctaccga tggttcgatg gataaacagg atattgccga
    27121 ttacgttgcc agtaatattc aggacccgtt aagccgcgtg aatggcgtcg gggatatcga
    27181 tgcctatggt tcgcaatatt ccatgcgtat ctggctggac ccggcgaaac tcaacagttt
    27241 ccagatgacg gctaaagatg tcactgatgc cattgagtca cagaacgcgc agattgcggt
    27301 tgggcaactt ggtggtacgc cttccgtcga taagcaggcg ctcaacgcca ccattaacgc
    27361 ccagtcactg ctgcaaacac cagaacagtt ccgcgatatc accttgcggg tgaatcagga
    27421 cggttcagag gtaaggctgg gcgatgtcgc caccgtcgaa atgggggcgg aaaaatacga
    27481 ttatcttagc cgcttcaatg gtaagccagc ctccgggctg ggggtaaaac tggcctccgg
    27541 cgcgaacgaa atggcgactg cgcagctggt gctcaatcgt ctcgacgagc tggcgcagta
    27601 ttttccacac ggactggaat acaaggtggc gtatgaaacc acctcgtttg ttaaagcctc
    27661 cattgaagac gtggtgaaaa cgctgctgga agctatcgct ctggttttcc tcgttatgta
    27721 tctgttcctg caaaacttcc gcgccacgct gatacccact atcgccgtgc cggtggtgtt
    27781 gatgggaacc ttctccgtac tttatgcctt cggttacagc gtcaacacct taaccatgtt
    27841 cgcgatggtg ctggcgatcg gtctgctggt ggatgatgcc atcgtggtgg tggaaaacgt
    27901 cgaacgtatt atgagtgagg aaggactcac tcctcgcgaa gccacacgta aatcgatggg
    27961 gcagatccag ggggcactgg tcgggattgc gatggtgctt tcggcggtat ttgtgccaat
    28021 ggccttcttc ggcggcacca ccggtgccat ctatcgccag ttctctatta ccattgttgc
    28081 ggcgatggtg ctgtcagtac tggtagcgat gatcctcact ccggctctgt gtgccacgct
    28141 acttaagcca ctgaaaaaag gtgagcatca cgggcaaaaa ggcttttttg cctggtttaa
    28201 ccagatgttt aaccgcaacg ccgaacgcta cgaaaaaggg gtggcgaaaa ttctccaccg
    28261 tagcctgcgc tggattgtga tttatgtcct gctgcttggc ggcatggtgt tcctgttcct
    28321 gcgtttgccg acgtcgttct tgccacttga agaccgtggc atgtttacta cctcggtaca
    28381 gttgcccagc ggttcaaccc aacaacagac cctgaaagtc gttgagcaaa tcgagaaata
    28441 ctacttcacc catgaaaaag acaacatcat gtcggtgttt gccactgttg gttctggccc
    28501 tgggggtaac ggacaaaacg tggcgagaat gtttatccgc ctgaaagact ggagcgaacg
    28561 cgacagtaag accggcacct cgtttgccat tatcgagcgt gcaacgaagg cgtttaacaa
    28621 gattaaagaa gctcgcgtta tcgccagcag cccgccagca attagcggtc ttggtagttc
    28681 tgcaggtttt gatatggagt tgcaggacca cgctggagcg ggtcacgatg cgctgatggc
    28741 agcacgtaac cagttgctgg cgctggcggc ggaaaacccg gagctaaccc gtgtgcgcca
    28801 taacggcctc gacgacagtc cgcagttgca gattgatatc gaccagcgta aagctcaggc
    28861 gctgggcgtt gctatcgacg atattaacga cacactgcaa accgcctggg gttcgagcta
    28921 tgtgaatgac tttatggatc gcggtcgcgt gaagaaagtc tatgtgcagg cagctgcgcc
    28981 gtatcgcatg ctgccagatg acatcaatct ctggtatgtc cgaaataaag atggcggcat
    29041 ggtgcccttc tctgctttcg cgacctcacg ctgggagaca ggctcgccgc gtctggaacg
    29101 ctataacggt tattctgcgg tagagattgt aggggaagcc gcaccggggg tcagtaccgg
    29161 tacggcgatg gatattatgg aatcgttagt gaagcagttg ccaaacggct ttggtctgga
    29221 gtggacggcg atgtcgtatc aggagcggct ttccggcgcg caggctccgg cgctgtacgc
    29281 catttccttg ctggtggtat tcctgtgtct tgcggcgttg tatgaaagct ggtcggtgcc
    29341 gttctcggta atgctggtcg tgccgctggg ggttatcggc gcgctgctgg caacctggat
    29401 gcgcgggctg gaaaacgacg tttacttcca ggtgggcctg ttaacggtca ttggtttatc
    29461 ggcgaaaaac gccatcctga tcgtcgagtt tgctaacgag atgaaccaaa aaggccacga
    29521 cctgtttgaa gcgacgctcc acgcctgccg tcagcgttta cgcccgattc tgatgacttc
    29581 gctggcattt atcttcggcg tattgccaat ggcaaccagc acgggtgccg gttccggtgg
    29641 tcagcatgcg gtgggtactg gcgtaatggg cgggatgatt tcggccacta ttctggctat
    29701 ttacttcgtg ccgctgttct ttgtgctggt gcgccgccgc ttcccgctga agccgcgccc
    29761 ggaataagca ataaaaaagg cgacatgcta atgtgtcgcc tttttcaact ttccgattaa
    29821 gaacctgctc agcgggttct tgctgtttgt actttgtctc aggaattact tacgaagcat
    29881 aacttcgata aagtctttcc agttccccag ttcacgttca atcataacaa cctctcttat
    29941 aattatgggt attctacgga aacaatatac cgtggtgaag ctaatttact cgattgctgc
    30001 gatgactacc tccgggggac aaaccttatg taaatactat ggtcctgcgg tgataatttg
    30061 tatgtgatac acagcaa
//
LOCUS       DAZKGF010000041        30045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZKGF010000041 DAZKGF010000000
VERSION     DAZKGF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.041"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            95..1315
                     /locus_tag="ACHOQV_001784"
     CDS             95..1315
                     /locus_tag="ACHOQV_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9630173.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDMPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1288..1917
                     /locus_tag="ACHOQV_001785"
     CDS             1288..1917
                     /locus_tag="ACHOQV_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP9630174.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLIPNDYNPNNVAPPEKKLLKKSIETDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1918..3078)
                     /gene="ybdL"
                     /locus_tag="ACHOQV_001786"
     CDS             complement(1918..3078)
                     /gene="ybdL"
                     /locus_tag="ACHOQV_001786"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFP9630175.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALSESRLEILPCEGTYFLLVDYSAVSSLDD
                     VKFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3187..4275
                     /gene="hcxA"
                     /locus_tag="ACHOQV_001787"
     CDS             3187..4275
                     /gene="hcxA"
                     /locus_tag="ACHOQV_001787"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFP9630176.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQKQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTEAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFENVESFKA"
     gene            complement(4285..4482)
                     /locus_tag="ACHOQV_001788"
     CDS             complement(4285..4482)
                     /locus_tag="ACHOQV_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP9630177.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4595..6700)
                     /gene="cstA"
                     /locus_tag="ACHOQV_001789"
     CDS             complement(4595..6700)
                     /gene="cstA"
                     /locus_tag="ACHOQV_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFP9630178.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALVALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6881..7294)
                     /gene="entH"
                     /locus_tag="ACHOQV_001790"
     CDS             complement(6881..7294)
                     /gene="entH"
                     /locus_tag="ACHOQV_001790"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFP9630179.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7297..8043)
                     /gene="entA"
                     /locus_tag="ACHOQV_001791"
     CDS             complement(7297..8043)
                     /gene="entA"
                     /locus_tag="ACHOQV_001791"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFP9630180.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8043..8900)
                     /gene="entB"
                     /locus_tag="ACHOQV_001792"
     CDS             complement(8043..8900)
                     /gene="entB"
                     /locus_tag="ACHOQV_001792"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFP9630181.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDSLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8914..10524)
                     /gene="entE"
                     /locus_tag="ACHOQV_001793"
     CDS             complement(8914..10524)
                     /gene="entE"
                     /locus_tag="ACHOQV_001793"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFP9630182.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10534..11709)
                     /gene="entC"
                     /locus_tag="ACHOQV_001794"
     CDS             complement(10534..11709)
                     /gene="entC"
                     /locus_tag="ACHOQV_001794"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFP9630183.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFAGAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11898..12854
                     /gene="fepB"
                     /locus_tag="ACHOQV_001795"
     CDS             11898..12854
                     /gene="fepB"
                     /locus_tag="ACHOQV_001795"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9630184.1"
                     /translation="MRLVPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAETVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLASGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12858..14108)
                     /gene="entS"
                     /locus_tag="ACHOQV_001796"
     CDS             complement(12858..14108)
                     /gene="entS"
                     /locus_tag="ACHOQV_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFP9630185.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QIMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14219..15223
                     /gene="fepD"
                     /locus_tag="ACHOQV_001797"
     CDS             14219..15223
                     /gene="fepD"
                     /locus_tag="ACHOQV_001797"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFP9630186.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLYTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15220..16212
                     /gene="fepG"
                     /locus_tag="ACHOQV_001798"
     CDS             15220..16212
                     /gene="fepG"
                     /locus_tag="ACHOQV_001798"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFP9630187.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAITLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAASLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLVADLCAQLLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16209..17024
                     /gene="fepC"
                     /locus_tag="ACHOQV_001799"
     CDS             16209..17024
                     /gene="fepC"
                     /locus_tag="ACHOQV_001799"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP9630188.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTTPSTAKI"
     gene            complement(17021..18154)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOQV_001800"
     CDS             complement(17021..18154)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOQV_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFP9630189.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEVDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQALEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSTPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKTNVNDVNFTPFKYQLRPSLPVKKD
                     GPGKAIIVILSALIGGIVACGGVLLRHAMASRKQDAIMADHLV"
     gene            complement(18370..22251)
                     /gene="entF"
                     /locus_tag="ACHOQV_001801"
     CDS             complement(18370..22251)
                     /gene="entF"
                     /locus_tag="ACHOQV_001801"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFP9630190.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPETIDLRTNIDPHGTAQSLMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYAEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPHAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTSLCYNAPLTPQGSAPLKLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFVRQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DSQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERALQEGMNPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(22248..22466)
                     /gene="ybdZ"
                     /locus_tag="ACHOQV_001802"
     CDS             complement(22248..22466)
                     /gene="ybdZ"
                     /locus_tag="ACHOQV_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFP9630191.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQVSCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(22469..23671)
                     /gene="fes"
                     /locus_tag="ACHOQV_001803"
     CDS             complement(22469..23671)
                     /gene="fes"
                     /locus_tag="ACHOQV_001803"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFP9630192.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFRWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGQVSAEG
                     LRIVLEAGIREPMIMQANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            23914..26154
                     /gene="fepA"
                     /locus_tag="ACHOQV_001804"
                     /pseudo
     CDS             23914..26154
                     /gene="fepA"
                     /locus_tag="ACHOQV_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
     gene            26264..27612
                     /locus_tag="ACHOQV_001805"
     CDS             join(26264..26744,26744..27612)
                     /locus_tag="ACHOQV_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9630193.1"
                     /translation="MKHSFEVKLAAVNHYLAGHAGIISTAKLFQLSHTSLSHWINLFL
                     LHGPRALDCRHKRSYSPEDKLCVVLYALGHSESLPRVAARFNIPSHNMVKNWIKGYRK
                     SGNEAFIRRRKEKSMTRSDDTHENEANMTPEEMKNELRYLRAENAYLKAMQEHLLEKK
                     PPGAGEKTKVIRSLKCGHCQSDLLKAAGLARSTLYYQLSLQKAKDKYADVKQLIASIF
                     HEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAE
                     NILQRQFKAEAPCEKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVK
                     RMLNKGLESLAEGEKPLLHNDQGWHYRIKSYQSALADKGLVQSMSRKGNCLDNAVMEN
                     FLGHLKEEMYYRRDYRSVEELENAVNEYITYWNQKRIKLSLGGLSPLEYRTEYQKAG"
     gene            27766..28386
                     /gene="entD"
                     /locus_tag="ACHOQV_001806"
     CDS             27766..28386
                     /gene="entD"
                     /locus_tag="ACHOQV_001806"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFP9630194.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFEPASFHEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAVHALREFGFKYAPPVGERRQPLWPAGLYGSISHCQATALAVVSHQ
                     PTGIDIEEIFSAQAAEELKDSIMTPAEYELLVVSNLPFSVVLTLAFSAKESAFKASKL
                     QTDQGFLDYQIVNWNKQRIIICWENEIFAVQWQIRGKQLITLCQHD"
     gene            28730..29665
                     /locus_tag="ACHOQV_001807"
                     /pseudo
     CDS             28730..29665
                     /locus_tag="ACHOQV_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(29813..>30045)
                     /locus_tag="ACHOQV_001808"
     CDS             complement(29813..>30045)
                     /locus_tag="ACHOQV_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein acyltransferase"
                     /protein_id="HFP9630195.1"
                     /translation="NGVVITQSDYIRLNNGRGYKTTLVNGSLLAKFSVAPNTKLINVQ
                     NYGGTIVFNNDWEIDWDIYNKSKKEHFFVVTL"
BASE COUNT         7312 a         8237 c         7771 g         6725 t
ORIGIN      
        1 atataaaata atgtgctcta tcttgcttcg tttatcctat aattagcgga ttcatctcac
       61 gagtcacccc gtaacgttca gagagcgttt ttagatgtct atttataaaa tcccgcttcc
      121 actgaatatt ctggaagctg caaaagaacg tattacctgg acacttaata ccctaccccg
      181 tatctgtgtt tctttttcgg gtggtaaaga ttccggatta atgctgcatc tgacagccga
      241 aattgcccga caaatgggca aaaaaatctg cgttttgttt atcgactggg aagcgcagtt
      301 ctcatgcact attaactatg ttcagtcatt acgtgagttt tacgccgatg tcatcgaaga
      361 gttttactgg gttgcgctcc cgcttacgac gcaaaattcc ctttctcaat accaacccga
      421 atggcagtgc tgggaacctg atgtcgaatg ggtgcgtcag cccccgcaag atgcgataac
      481 cgatcctgac tttttctcct tttaccagcc tggcatgacc ttcgaacaat ttgtgcgtga
      541 gtttgctgaa tggttttcac aaaaacgtcc ggcggcgatg atgatcggca tccgtgcaga
      601 tgagtcctac aaccgttttg tcgccatcgc cagtttaaat aaacaacgtt ttgccgatga
      661 tatgccctgg accaccgccg caccaggcgg tcatagctgg tacatttacc ccatctacga
      721 ctggaaagtg gctgatatct ggacctggta tgctaaccat caacagctct gtaacccact
      781 gtataatata atgtatcagg caggcgttcc tctgcgtcat atgcgaattt gcgaaccttt
      841 tggcccggag caacggcaag gattatggct ctatcacgtt atcgaaccgg atcgctgggc
      901 tgctatgtgc gcacgagtca gcggggtaaa aagtggcggc atttacgccg gacatgataa
      961 tcatttttat ggtcatcgga aaatcctcaa gccagaacat ttagactggc aagaatatgc
     1021 attattgctg ctcaatagca tgccggaaaa aacagctgag cattaccgca ataaaatcgc
     1081 catttatttg cactggtatc agaaaaaagg catcgaagtt ccacaaaccc agcaagggga
     1141 cattggcgcg aaagatatcc cctcctggcg gcgaatatgc aaagttttac tcaataacga
     1201 ttactggtgc cgggcattat catttagtcc tacgaaagcg aagaactatc agcgttataa
     1261 cgaacggata aaaggaaaac gtcaggaatg ggggatacta tgcaacaacg attaacgcag
     1321 gatttaaccc aatttctcgc cagcttgccg gaagatgatc gcatcaaagc gattaatgaa
     1381 atccgcatgg cgatccatca ggttagcccc tttcgtgaag agccagtgga ttgcgttctg
     1441 tgggttaaaa acagccaact tatacctaat gattacaacc caaacaacgt ggcaccaccc
     1501 gaaaaaaaac tgctgaaaaa atccattgaa acagatggtt ttacccaacc cattgtggtc
     1561 acgcatacgg ataaaaatgc cctggaaatc gtggatggtt ttcaccgcca tgaaattggt
     1621 aaaggctcat catcgttaaa attacgctta aaaggttatt tgccggtgac ctgtctggaa
     1681 ggtacgcgta atcaacgcat cgccgcgact attcgtcata accgcgctcg tggtcgccac
     1741 caaatcactg ccatgtccga aattgttcgt gaactcagcc agttgggatg ggacgataat
     1801 aaaattggca aagagctggg aatggacagc gacgaagtgc tgcgcctgaa acaaattaac
     1861 ggcctgcaag agttgtttgc cgaccgtcaa tattcccgtg cctggactgt caaataacta
     1921 cagctggcgc aggcgttcag ctgctgccag caacgtcgat tccttcttgg caaaacagag
     1981 acgaatcagt ttatggggga agggatcggc gcaaaatacc gacagcggga tcgccgctac
     2041 accgtgctcc cgcgtcagcc actggcaaaa cttaacgtca tctagcgaag aaaccgcgct
     2101 gtaatccacc agcaaaaagt atgtaccttc gcacggtaaa atctccagcc gactctcact
     2161 taaggcattc accagaatat cgcgtttctg gcgataaaag tccggtaacg caagataatg
     2221 ctcaggttct gcacgtagca tatcggcaag cgccaactgt gccggggtat tcaccgaaaa
     2281 ggtcagatac tgatgcactt tgcggatctc ggcgctgatg ggcgcaggcg caacgcaata
     2341 acccactttc cagccggtca tatgataggt cttgccaaac gatgacaccg ccaccgctcg
     2401 ctcacgcaat tgcggatgcg ccagcacgct ggcatggccc tgttgtgaaa aattgatgtg
     2461 ctcgtagact tcatcgctaa tgacaaaaat ctcgtgcccg gcgatcgcct gccacaaagc
     2521 ggcgaaatca gcctgctgcc agacagttgc actggggtta tgcggggtgt tgaggatcac
     2581 cagtcgggtg cgctcgctta acaatgcggc aaattcctgc cagtccacgc gaaaatgcgg
     2641 tggttgcagt gcaatacgtt tcactattcc ccccgaaagc gcgatggcgg gcgcgtaact
     2701 gtcatagctg ggatcaaagc agatcacttc atcaccattg cgcaccaatg cggtaatcgc
     2761 tgcatataac gcttccgtcg cccctgccgt tacggtgata tcgctatcgg catccggttg
     2821 atagccatac agacgttccg ttttctgagc aatcgcctcg cgcaaggcct gcacgccggt
     2881 catgggcgcg tattggtttg ccccctgggc aacgtggtac gccagccgct cctgtaaata
     2941 gcgcggacca tcaaaatcag gaaagccttg tgaaaggtta atcgcctggt gttgctgcgc
     3001 cagcgcgctc atccgggtga aaatagtggt gccaagttgt ggaagtttgc tttgtggaat
     3061 cagagggtta tttgtcattt ttattgtacc tgacgaagtt gcgtgttgaa ggcactataa
     3121 cacgatgcta gtatttggca atcaagacgt ttagatgtct aaataaaaca ataaggacaa
     3181 cacaacatgc ctcacaatcc tatccgcgtg gtcgtcggcc cggctaacta cttttcacat
     3241 cccggaagtt tcaatcacct gcacgatttt ttcactgatg aacaactttc tcgcgcggtg
     3301 tggatctacg gcgaacgcgc cattgctgcg gcacaaacca aactgccgcc tgcgtttgaa
     3361 ctgccagggg taaagcatat tttgtttcgt ggtcattgca gcgaaagcga tgtgcaacaa
     3421 ctggcggcgg agtccggtga cgatcgcagc gtggtgattg gcgtcggcgg cggcgcacta
     3481 ctcgacaccg cgaaagccct cgcccgccgt ctcggtctgc cgtttgttgc cgttccgacg
     3541 atcgccgcca cctgcgccgc ctggacaccg ctctccgtct ggtataacga tgccggacag
     3601 gcgctgcatt atgagatttt cgacgacgcc aattttatgg tgctggtgga gccagagatt
     3661 atcctcaatg cgccgcaaga atatctgctg gcggggatcg gtgacacgct ggcgaaatgg
     3721 tatgaagcgg tggtgctggc tccgcaacca gaaacgttgc cgctaaccgt gcggctgggg
     3781 atcaataatg cgcaggccat tcgcgacgtc ttgttaaaca gtagcgaaca ggcgctgagc
     3841 gatcagcaaa aacaacagtt aacgcaatca ttttgcgatg tggtggatgc tattattgct
     3901 ggtggtggga tggtcggtgg tctgggcgat cgttttacgc gtgtggcggc ggcccatgcc
     3961 gtgcataacg gtctgaccgt tttgccgcaa accgagaagt ttctccacgg caccaaagtc
     4021 gcctacggaa ttctggtgca aagcgccttg ctgggtcagg atgatgtgct ggcgcaatta
     4081 acagaggcgt atcagcgttt tcatctgccg actacgctgg cggagctgga agtggatatc
     4141 aataatcagg tggagatcga caaaatgatt gcccacaccc tacgtccggt ggaatccatt
     4201 cattacctgc cagtcacgct gacaccagat acgttgcgtg cagcgttcga aaacgtggaa
     4261 tcgtttaaag cctgacgtac aaatttagca gcagcgcgcg ccgccttttc caccgtagcg
     4321 cgcgtcctgc cgctcccgga aaaactcctc gtaggtcatc ggtgtttgat cgggatggtt
     4381 aacccgcata tgttcgacat agttgtcgta atcaggcata ccaatcatca gtttcgccgc
     4441 ctgacctaaa tattttccgg cttttgccag tgaatcaaac atctaaacct ccggtaatac
     4501 aaatgccctc ctgaattaag agggcttaat tgctatcaac acgctcggga tgagagtaag
     4561 gggaacgctc agcacattcc ccctcactct gactttagtg cgcgcctttc gcctgcgcca
     4621 cgatctcctc gacattttcc ggcatcggct catatggcgt ttctttcgcc gttggcttcg
     4681 gctctttcaa tgccaccagt gccgtcttaa tcgagaacag cgccagaacc accacaacca
     4741 ccataaagaa gatggttaat ccggcatcca gacggttgtt gaacaccagt tgcgccagtt
     4801 gtgactcagt atactgcgac ggaatattgc cgctgtcgat cattgcctgg aacttgttag
     4861 caatcgccag gaagccgact ttcgcatccg ggctaaacgc tttctgccag cctgcggtca
     4921 gggtacaaat cagcagccag gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt
     4981 tcattttgaa cagcaccaca gcacagagca tcagcgccat ccctgccagc atctggttgg
     5041 caataccaaa cagcggccac aaggtgttaa taccgcccag cggatcgacc acgccctgat
     5101 gcaggaagta gccccacgcc agcacgcaca gcgccgttgc cagcaggtta gcaggcagtg
     5161 aatcggtccg tttcaggccc ggagacacca cgcccagcag atcctgcaac ataaagcgcg
     5221 cagcacgcgt acccgcatcc accgccgtca gaataaacag tgcttcaaac agaatggcga
     5281 agtgatacca gaacgccaca tccatcatgc cgcccagcgc accgtgcaga atgtaggcca
     5341 tccccaccgc cagggtcggc gcaccgcccg cacgagaaat gatcgactgt tcacccactt
     5401 cgctggcaat ctggtttagc gtatccgggg taatcgcaaa gccccagcta ctcaccacct
     5461 gcgcggcaga agcaaccaca tccgccgtcc ctgccggagc cagcaccgcc atcgggctgt
     5521 tcatggcgaa gtacacaccc ggatcgatga tacaggcgga aaccagcgcc ataatcgcca
     5581 cgaaggattc catcaacatc ccaccgtagc cgataaagca cgcctgccct tcgttcgcca
     5641 gcatcttcgg tgtagtgccg gaagagatca gtgcatggaa gccagacacc gcgccacagg
     5701 cgatggtgat aaacaggaac gggaacaggt taccggtcca taccgggcca gtgccatcga
     5761 caaatttagt tagcgcaggc atggtcagcg tcgggcgcat aatcaaaatg cccaccgcca
     5821 gaccgacgat agtcccgatt ttcaggaagg ttgagaggta gtcacgcgga gccagcagca
     5881 accacaccgg cagcaccgcc gccacaaaac cgtaacccac cagcatccag gtcagctgca
     5941 cgccggtaaa gtcaaagtac ggtgcccacg tcgggctttc tgccacccag ccgccagaga
     6001 taatggcgaa aataaggaac accagaccaa tcaccgacac ttcgccaata cgccccggac
     6061 gcaaatagcg caggtagatc cccataaaca gcgccagcgg aatggtgaac gcaacggtat
     6121 acgttcccca cgggctatga gtcagggctt tcaccacgat catcgccagc acggcaagga
     6181 taatgaccat gatcataaag caggccacca gcgcaatcac cccggcggtt ggccccatct
     6241 cttctttgac caactcaccc agcgagcgac catcgcggcg cgtagaaaca aacagcacca
     6301 tgaaatcctg caccgcaccg gcgagaacca ccccagcgag cagccagatc atccctggca
     6361 ggtaacccat ttgcgccgcc agtaccggcc caaccaacgg acctgctccg gcaatggccg
     6421 caaaatggtg accgaacagc acttttttgt ccgtcggcac atagtccagc ccgtcgttat
     6481 ggcgcaccgc tggcgtcata cgcgtcgggt caaccgccag cacatttttg gcgatataca
     6541 gcccataaaa acggtaagcg atcagataga tacagaccga cgccaccaca atccacagcg
     6601 cgttgatctg ttccccacga tttaaagcaa tgtatcccag agcaaatgct cccattacag
     6661 agagcactgt ccagacgagg tatttccctg atttgttcat agttgttatc cgtgtgcgtg
     6721 tccatagaga tgttacattt tgtttctata acatctttgt acaatttaac aacacggcaa
     6781 ccatgtaaat cggagttgaa accaatattt aacttaacaa tgttaactcg atcactccgt
     6841 taaccgagtt ttcctgcaat ctcatttaat tctgtcgggc tcatcccaaa actgccgtac
     6901 ccagccgaca agtgcagcaa cgccgcccct gttcatcgaa aacaacaatt tcccagctct
     6961 gattttgccg accaagatgc agcggctggc agacgccgcg tacctttccc tcagacaccg
     7021 ggcgatgatg tgtggcgtta agttccgtgc ccaccacaca ctgtccgtcg cgagtcatca
     7081 taaatccggc catcgatccc agcgtttccg ccagcgccgc cgacgcgccg ccatgtagta
     7141 aaccgaacgg ctgatgagta cgggtatcaa ccggcatttc ggcttccagc acatcatcgc
     7201 ccaggcgggt atacacaatt cccagatgcg ccaccattgt gttatcgctg gtggcgttca
     7261 gctcgtcgag cgttaaatgg cgtttccaga tcatgcttat gcccccagcg tagagccgcc
     7321 atctaccaca atatcctgta gggtgatatg gctggcgagg tcagaggcga ggaacaaaat
     7381 cgtgttggcg atctcttgtg gacgggcgat tttccccagc ggaatgccaa gtttaaactg
     7441 ctcgccaaag ccgcgaatac gctgctcttc ggcgtcatcg ctcacccaca gcgtgcgttg
     7501 catatcggtg tcggtggagc ctggcgaaac cacattacag cgcacgccgc tacccgccag
     7561 ttccagccca acgctcaacg ccaggctttt cagcgccgct ttcgacgcgc cataagcact
     7621 cataccaatg cgcggcgtgt gtgcggcgtc ggacgcaaca gtaacaatcg ccccgccccg
     7681 ctgacggcga aactggttca tggtctgctg gaacaggtta aacgcaccgc cgacattaac
     7741 cgcaaaagtc tgctgccagt cctccttact gagctgatcg gtcgcgccca tgcgcaaaat
     7801 tcccgccgca ttgaccagca cgtccagtcg ctccgtttca gctaacagtc gctgacatac
     7861 ctgcgccacc tgcgcagcgt cggcaacatc catcacttcg gtcgcaaagg gatattgctc
     7921 ctgagtgaac gcttgatcaa aacctgtaac tttcgctccc gcctcaacaa acgccagcgc
     7981 cgtggcgtag ccgatacctt tccccgcgcc agttacccag acatttttac cgctgaaatc
     8041 cattatttca cctcgcggga gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc
     8101 agcatgacaa agtcgatatc gccatgcact ttgcgccagc gcgccgccag cgccatcatc
     8161 cgcaccgaat ccagaccgta gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg
     8221 tccagcaacg gcaggatcac ctcacgcagc gccgctttgc tggcggggat aggtgctggc
     8281 agtaattctt cggtcatcac cactcggcca gaacgtccgg ccacatattt cagcgacatc
     8341 aaatgctcgt cacggctgaa atcggccagc gcatctgcca ccataaacgg tttaatatcg
     8401 cgcataaatg cgtcggttgc ggtggtcata cagccaatgt gggcatatac cccggtaata
     8461 atcagctggt tacgtccact ctctttcagc atttgctcca gcggggaacg atgaaacgcg
     8521 ctgtagcgcc acttcaccag caccgtgtcg tcggcatctg gcgtcaggct atccaccacc
     8581 ttttgctgtt ccggcgaacg ggtcaggcca ggcccccaca tatcattcaa cagcgcccga
     8641 tcttcatcgc tctgctcttt cggctgggcg gtgtaataaa ccgggatatt gtgctgtttg
     8701 cagtagtcgc gtagtgccgc aatattcgcg atcacctgct ccatcatcgg gcagttctcg
     8761 ccccagaagc tgacaaaata gtcctgcata tcatggatta acaacgcggc acgttgcggt
     8821 tcaaaggccc agtcgacttt attctgcgga atatcgtgag actccggcag tgcgtaagcc
     8881 tgtaattttg gaatagccat cgtgttctct ccttcaggct gatgcgcgtg acgccagcca
     8941 ctgacgtaat tgttttttat cgactttccc gaccgccgtc agcggaagtg aatctacaca
     9001 ctccacgcga tccggtaatt taaattcggc aataccctgt tcacgcagga aacgacgcac
     9061 ctgcaccgcg cgcagcggct cttttaccac cagataagca cagctttttt cgcccatcag
     9121 ctcatcttcc atgctcacca gtgcggcgta gatcaccgcc ggatggcgca gcagcaggtt
     9181 ttcgatctct tcggcagcga tcttctcgcc gccacggttg atctgatctt tctcgcgccc
     9241 ctgcacagtg atgtaaccct ctggatcaat agagatcaga tcgccggaac agtaaaaacc
     9301 gttggcatca aaggcgctgg cattgtgctg cgggcttttg taatagccac ggaaggtata
     9361 cggaccgcgg gtcatcaggc gtccaacttc cccttgcggc agtggatttc cttcggcatc
     9421 ggcaacccac acttcatcgt ccggacacat tgggtaaccc tgggtatgga taattttctc
     9481 cgcgctatca tcaagtcggg tgtagttcac tagtccttcc gccatgccga atacctgctg
     9541 taactggcag ccaatctctg cgggaatacg cgccgcaagc gtggcagaaa gacgtgcacc
     9601 accgacctgt aacagtttca gcgaggcaag ctgcgcccgg ctttcgcctt cagccagtgc
     9661 ctgcaaccac aggctgactg cgggcggcac cagcgcggta acgttgacct gatgtttttc
     9721 aatcaatggg aagcaaagcg tggcgctggg gtcggcagcc agaacgacag tgccgccagc
     9781 gagaaatacc cccaacgatc ccggcgaact catggcgtag ttatgagccg ccgggatcgc
     9841 gcacaggtag cgcgtctgtt gtgtgaactg acaaatctcg acgctacgac gcacgctgta
     9901 gtagtagtcg ttatgagtgc gcggtatcag tttcggagtg ccggtggtgc cgccggaaag
     9961 ctggaaatag gccacttcat cagcaggaga tggcgtggca gtaaaatcct cagccggatg
    10021 gttaatcgca tcctgcaagt tatgctcacc gctgtcgttg agcagctgca ccacgcgaat
    10081 ggaagaatgc tctgcaacaa atgtgttgag gaaatcatcc ccgctaaata gcgcatgttg
    10141 acgatcggca atcagtaacg ccggttcaat ctggctggca taggcgttca gttcactacg
    10201 ctgatggctg aacagcgcca gcaccggcgc aacgcccagc ttcagcagcg cgaaaaaggt
    10261 aatatacaat tcagcgacgt tacccagttg taccagcgcg gtttcaccag gtttaatgcc
    10321 ctgacggcgc aaactacagg cgaggttatc cgccgcctga ttcagctccc ggtaactcaa
    10381 ctgccgctcg ccgtcgataa ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc
    10441 ggtcagtggc aaatcctgcc agtagccttt ttcccgatag cgacgggcaa actcttccgg
    10501 ccagcgggtg aatggaatgc tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat
    10561 ggtagaaagt ttgacgcctg tttcgcgcca ctcacccaac ggtgaggacg caggcacaat
    10621 ccccgctccg gcaaacagac gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac
    10681 cacccattca ccgttacctt cgctgtcaca ccaacccaca atgccgccaa acagttcgcg
    10741 gtcgaacggc tccagttcag caataacctg agtcgcggcc tgatgcggga aaccgctcag
    10801 cgcgggggtc ggatgcagca gacaggccag agtcagtgcg ttttcttgcg aattcgcttt
    10861 accttcaaag ggagttgcga gatgccacag cgtcggcgtg gtaatcaatt gtggagagga
    10921 gggaacgtgt aactcactac tgcgtttgcg cagtacctct ttcatcgcct gagtcaccag
    10981 ttcatgttca tggcgatctt tttctgacgc cagcagacga ttacccgctt cgcgatcgag
    11041 cacttcatcc ggctgacgac gcgcggaacc ggctaacgga atggagctaa aacgctcgcc
    11101 gtctttacgt agcagcagtt ccgggctggc ccccagcagg acgccgccat cagccagcgg
    11161 gacatggaag ttgtaactaa ccgggttttg cgcaatcaac cgttccagca atacgccact
    11221 atcaatggcg gcgtcagtgg tgatatcaat caaccgtgac aacaccactt tgtcgacctg
    11281 cggcgtggcg gtaagtgcgg cggcgcgggc aaccatctgt tcaaacgtgg tttgttccgg
    11341 aattgcctgg cgttccacca cattcagcga ctggctgcgg gtgaaacggc gtgcggaagc
    11401 ttgtttttcc tgacgggaga acgactgcca ggattcggga atatacagcg atgaaggctg
    11461 acgtggatcg aagggaatcg ccccgaccat caccggattt ttgatgccct gcgctttggc
    11521 accggcaaac agcgcggcga gtttttgctg gaagggactg tcgggcgaat ccccgttcac
    11581 agccggttca tcgaagcggg cgaaacatcc tgacgtcgta aaactgcggt acggcgacat
    11641 aaagaaaaag cgattgggcg caagtgttgc catggtctgc tgtacttcct cagccagtga
    11701 cgtatccata tcatcctcca caaaatgata aaggctttaa taatgattat catttatatt
    11761 ttcggtcgct aacctaaaag caaacgcgca ctatgtcaac tcttgaggta acgcaatctg
    11821 caactcgggg ttgcatctgg ttgtggttac aatgaaaatg agaagcatta acttcattaa
    11881 taacaggaag ctgatttgtg agactcgtcc cgctctaccg caacgccctt ctattaacag
    11941 gacttttgct ttcaggaata gccgtagttc aggccgccga ctggccgcgt cagattactg
    12001 acagccgggg cactcatacc ctggaaagcc agccgcagcg tattgtttcc accagcgtca
    12061 ccctgactgg ctcactgctg gcgattgatg ctccggtgat cgccagcggc gcgaccacgc
    12121 cgaataaccg cgtcgcggat gaccagggct ttttacgcca gtggagcaag gtggcgaaag
    12181 aacgcaaact gcaacggctc tatatcggcg agccgagtgc agaaaccgtt gccgcgcaaa
    12241 tgccggatct gattttaatt agcgcaaccg gcggtgattc agcgctggcg ctgtacgatc
    12301 agctttccac catcgcccca acattaatca tcaattacga cgacaaaagc tggcagtcgc
    12361 tgttaacgca gcttggcgaa attaccgggc atgagaaaca agcggcagag cggattgcac
    12421 agtttgataa gcaactggcg gcggcgaaag agcaaatcaa attaccgccg cagccggtca
    12481 ctgccattgt ctataccgcc gctgcacaca gtgccaatct ctggacgccg gaatcagcac
    12541 aagggcagat gctggaacaa ctcggcttta cgttggcgaa attgcccgca gggttaaacg
    12601 ccagccaaag ccagggcaaa cgccatgaca tcattcagct tggtggggaa aatctggctt
    12661 cagggttaaa tggcgagtca ctattcctgt tcgccggtga tcagaaagat gccgatgcga
    12721 tttatgctaa tccgctgctc gcgcacctgc ctgcagtaca aaacaagcag gtttatgcgc
    12781 tgggaaccga gacgttccgt ctggattact acagcgccat gcaagtgctg gaacggctta
    12841 aggcgctgtt ttaagcatta actgtcggac gctgtcacct gcggcggcgt ctggcgaaaa
    12901 cgccgcaact ccaccagcac cagcaataac aacacgccga taatcaacaa accaaaaccg
    12961 ctcgcgcttg cggaagcaac cggtgtcatc atcgcgccca aaccgcccag cagcgccgcg
    13021 cctatggcat cgcccgtcac gttttgcgcc gtccacaaac cgttaatccg ccctaacatc
    13081 gcttccgggg tttgcgtttg cagcattgtg tattgcagca acgaactaac cgcgctcagc
    13141 cagccgaaca gcgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga
    13201 ccaatggcga ggaacgatcc cagcgtggaa agcagcatca ataaccctgg tcgcgcgcta
    13261 tgtgccagct tcccgctggt taacgcgcca atagccgcac cgagcgggat cgccgcatag
    13321 agaaaaccaa tctgtgccgc cgacatctgc cagttgtcag ccagcgccgg atacaggacc
    13381 cgcaccgcgc tcgccatcgt caataaacca cccaacagcg caatccctcc taccagcggg
    13441 ctggcgagca gaaaacgaaa tcctgccagt aatgatttca acggatgctc acgcggctgc
    13501 ggtggtggtg gtaacgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc
    13561 gccgccagcc cgtagttcca ggccacaccg ccagtcgcca gcagtaaacc gccaatcatg
    13621 ggcgaaatca ccgatcccag acgcacggtc aacatggtga tcgccccggc ctgcattaag
    13681 ttttcacgcc ctaccagtgc tggtgtcgcc gccagtagcg ccgtaacgcc aagtgatgcg
    13741 aaaaaaccat cccataaacc aagtaaatag attgccagca atgacggctc cggcagcagt
    13801 gcattaaggc acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact
    13861 tttttgcgct catagcgatc cgccagcaca ccgccgacca tcaggccaac aaacatcgcg
    13921 ccgccggtca gcgtcaccga aagccccacc tgccaggtag aatgcgtcat tatctggatc
    13981 tgcaccggta ccgcgacgcc gagcaaaccc agagacacaa ttgagataaa acgagcgagg
    14041 aatactgcgc gaaacgccgg gtgcgttttc aacaggctga ggttaagcag ccaggattgt
    14101 ttattcatta caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg
    14161 ctaacatatc caaataagat cgataacgat aattaatttc attatcatgg aagttcgtat
    14221 gtctggttct gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat
    14281 tatcgcgacg gcattaagcc tgctcattgg ggcaaaatca ctccccgctt ccgtagtgct
    14341 ggaggccctc tccggcacct gccagagcgc cgactgcacc atcgtgctcg acgcgcggct
    14401 gccgcgcacc cttgccggtt tactggcagg cggcgcgctt gggcttgccg gggcgttaat
    14461 gcaaaccctc acccgaaacc cacttgccga ccccggcttg cttggcgtaa acgccggagc
    14521 cagctttgcc attgtgctgg gtgcggcgct gtttggttac tcatccgcgc aggaacaact
    14581 ggcgatggcc ttcgccgggg cgctggtggc ctcgttgatt gttgccttta ccggcagcca
    14641 gggcggcggg cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt
    14701 gctggaaggg ctgaccagcg gcatcgccct gcttaatcct gacgtctacg atcaactgcg
    14761 tttctggcaa gccggttcgc tggatattcg caatctatat accttaaaag ttgtgctgat
    14821 cccggtgctg atcgccggag caaccgcgct attactgagc cgcgcgctga acagtttaag
    14881 cctcggcagt gacaccgcga cagcgctggg cagccgcgtg gcgcgcacac agttgattgg
    14941 tctgctggcg attaccgtgc tttgtggtag tgcgacggcg gtagttggcc cgattgcctt
    15001 tattggcctg atgatgccgc atatggcgcg ttggttggtt ggagcagatc atcgctggtc
    15061 gctgcccgtc acgctacttg ccacccctgc cctgctgctg tttgccgata tcatcgggcg
    15121 ggtgattgtg cccggcgaac tgcgagtttc cgtggtcagt gcgtttattg gtgcaccggt
    15181 gctgatcttc ctcgtgcgac gtaaaacgcg aggtggcgca tgatttacgt ctctcgccga
    15241 ttaattatca cctgtttgct gctggtttcc gcctgtgtgg ttgcaggtat ctggggatta
    15301 cgcagcggtg ccatcacgct ggaaacctcg caggtattcg ccgcgctgat gggcgatgcg
    15361 ccgcgcagta tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg
    15421 ttgattggcg cggcgctggg cgtcagcggc gcgatttttc agtcgctgat gcgtaacccg
    15481 ctcggcagcc ctgacgtaat gggctttaac accggggcat ggagcggcgt actggtggcg
    15541 atggtgctgt ttggtcagga tctgacggct atcgcgctgg cagcaatggc ggggggtatt
    15601 gtcacctcgc tgttggtctg gctgctcgcc tggcgtaacg gtatcgacac ctttcggttg
    15661 attattatcg gtatcggtgt tcgcgccatg ctggtggcct ttaatacctg gctgttgctg
    15721 aaagcgtctt tagaaacggc gctaacagcg ggtttgtgga atgccggatc gctcaacggc
    15781 ctgacgtggg caaaaacctc accttccgcg ccaatcatta tattgatgct cattgccgcc
    15841 tccttgctgg tgcggcgaat gcgcttgctg gaaatgggcg atgataccgc atgtgcgctg
    15901 ggcgtcagcg tcgaacgttc gcggctgtta atgatgctgg tcgccgtagt gcttaccgct
    15961 gcggcaacag cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc gcacattgcc
    16021 cgccgcatta gtggcaccgc tcgctggggg ctaacccagg cggcgctgtg cggggcgttg
    16081 ttactgctgg tggccgatct ctgcgcccaa ctactgttta tgccgtatca acttccggtt
    16141 ggcgtcgtta ccgtcagcct cggtggtatt taccttatcg tcttgttaat tcaggagtct
    16201 cgcaaaaaat gaccgaatca gtagcccgtt tgcgcggcga acagttaacc ctcggatatg
    16261 gcaaatatac cgttgcggaa aatctgactg tagaaatacc tgatggtcac ttcacggcaa
    16321 ttatcgggcc aaatggctgc ggtaaatcca cgttgctgcg taccttaagc cgcctgatga
    16381 cgcctgctca cgggcatgtc tggctggatg gcgagcacat tcaacattac gccagtaaag
    16441 aggttgcacg ccggattggc ctgttggcgc aaaacgccac cacgccggga gatatcaccg
    16501 tgcaggagct ggtggcgcgt gggcgttacc cgcatcaacc gctgtttacc cgctggcgca
    16561 aagaagatga agacgcggta acgaaagcga tgcaggccac gggaatcact catctggcag
    16621 atcaaagcgt ggataccctt tctggcggac aacgccagcg agcgtggata gcaatggtgc
    16681 tggcccagga aacggcaatt atgctgctcg atgaacccac gacctggctg gatatcagtc
    16741 atcagattga tttgctggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg
    16801 cggcggtgct gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc
    16861 gggaagggaa aattgttgct cagggagcgc cgaaggagat tgtcactgct gaactgatcg
    16921 agcgcattta tggtctgcgc tgcatgatta ttgacgatcc ggttgccgga acgccgctcg
    16981 tggtgccgct cggacgaacg acaccttcaa ccgcgaagat ttaaactaag tggtctgcca
    17041 ttatggcatc ctgttttctg gatgccatcg catggcgcaa tagcacgcca ccacaagcca
    17101 ctataccgcc gatcaacgca gaaaggatca caataatcgc cttacccgga ccgtcttttt
    17161 ttacgggtaa tgacggacgc aactgatatt taaacggtgt aaaattcaca tcgttgacat
    17221 ttgtttttgt taattgctcg actagatact gacgattacg taattcaccg ttcaattccg
    17281 caacgtcagt gactgctttt tcaatttcca gtttgcgttc aataccgtct gcgccgagag
    17341 aaatagagaa atcggggtca tctttaacgg cctgaccatt actgtatacg ggctttttaa
    17401 ttcctgccgc gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt
    17461 gatttttcgt tttaatgcga tcctgagcca gtttttcttt ttcaaactgg gttttgatct
    17521 ccagtttatt acggacgttt tctatcgact ctttcaccac caacgcagag atataatcga
    17581 tatacccggc caacactttc tgtgcctctt cactggtggg tgcggtgaaa cttagcgtcc
    17641 aggaagtata caatgcagac tcatcttttt tcttactggc attgtcatca accgctttca
    17701 ttttttcgct caatgcgaca attgcgcgat gcaaatccag ttcgtcgatt ttcgcctctt
    17761 ttaattggtc catcacataa ggtgttgagc gcaggtactc ttccagcaag ctaaccgact
    17821 gaaacttctt gataaacagg ttaaatgctt ctgtacgatc aattttgata tccagatcca
    17881 gcacacgaag cttagtgaat gttttctcaa gcgcctgcca ctgaacaggt tctggaggcg
    17941 tgacaaccgc cgcgctggtc catttttgcg gcaggatgaa agagatcagt aaacctgcgc
    18001 aggcaaacgc aaaaacgacc gccatgaccg tttttttggc ccgccataaa acttcaatta
    18061 gattaagtaa atcaacttca ttattactgg gcgacgccag aggatactcg ggaaaatgag
    18121 cttcacttcc ctgtttaata ttcagtgatg acatggggta taacctgaaa atgagtccaa
    18181 taagagtaaa tctccacggc ccccactcta ccagttggtg agatttatcc gaagctgaag
    18241 tttgtaaatt actcccactg aatgcaacat tcactctatt tcagagaaat acccaccaaa
    18301 tttataaatt aaataaatga taaacccata attatttcct gtaattatgg gtttatagat
    18361 aatattaatt tacctgttta gcgtagcgcg aataatcggc ccaatttttt caaacgcccc
    18421 tggagagata atatccacat gcgcacaatc ctgacgatag atatccagct cggctatcca
    18481 cggcgaccag gcgcgttcgg gattcatacc ttcctgaagc gcgcgttcag caacaaacag
    18541 cgtcgcttta ccgtcaaacg gtacactatg agcagtcgtc agcaggcgca cagcatcagc
    18601 gtagttgcct tcaatggtgg taaacaactc cgttgaagta cttccctgct gtgccgccag
    18661 gaaggcctcg cgctcgcggt taatctccgc cagcacttcc gggtccagac cattggcttc
    18721 tttttcctgc cagttttgcg tttctggcgg ccaggtatcc agcaagccaa gaaatgccac
    18781 ctgttcgcca cgggcacgca gtcgcgccgc aatgccctgc gccagcgtac cgccaagtga
    18841 ataacccagc aggtaataag gaccgtgcgg ttgttgttca agtaacgttg ccagatgcgc
    18901 ttcgcagact tcatccaggt ttgccgccgt ctgcatgggg ccatgagggc gcggcgactg
    18961 aatgccgata atcgaccatt gtgaatcgag ataacgcgag agcacgctaa actgccaggc
    19021 aaaaccggag gccggatgga aacaaaacag cgtcgggcca ttaccttcac gcaacggcag
    19081 aatggtttcg aatcccatgc gctgggtgct gtcttcttca ccatcaataa tcgttgccag
    19141 tttggcgaca gttgacgcga ccatcacctg ccccggcgtc acctgacgaa caaactgtcg
    19201 acttaactgc gctgccagtt tcattgccag tagcgaatga ccgccaagcg caaagaaatc
    19261 agcatcggca tcctgcacgt cacaacccag caacgacgag aatgccgcgg cgataatcgt
    19321 ttcactgccc gctttcggcg cacgccccgg cgcttgtgct ttcagttccg gcaacggtaa
    19381 ggctttgcga tccagcttgc cgttggcgct aagaggtaac tgtggaagtt gcagcagaac
    19441 cacgggcacc atatgtggtg gcaatgtttc gcgaagctga gcctgtaatg cgctggtatc
    19501 caacggcagg cccgattgcg acaccagata gcccaccaac tgacgcgcat caccaccagt
    19561 ggctgccgcc tggttaatca cacaggcgtg ggtaacggct tgttcgacat ccggcagcgc
    19621 ctgcatcacg cgatcgattt cgcccagttc gatacgctgc ccgcgaattt ttagctgatc
    19681 gtcactgcgc ccgaggtact ccaccgcgcc gttatccagc cagcgggcaa cgtctccggt
    19741 acggtacatc cgttcgccag gggcaaaagg atcggcaata aagcggctgg cggtcagatc
    19801 gggtcgtcca agatacccct gcgccagttg aataccggtg agatagagat ctcccgccac
    19861 acccggcggc accggatgca tcatcgcatc aagaatacgc aggcccgtat tccacaccgg
    19921 ataaccaatc ggcacactgc tgccgcgcac ctgtgccagt tcctcgccaa aagccggata
    19981 ccagctgaca tctaccgccg cttccgtcgg gccatataga ttatgcaacg gcacgctggt
    20041 taactgttgc cattcgcggc ataaatcagc gggtaaggcc tcaccactac agaaaacctg
    20101 tttcaacgtc gcgcaattct ggcgagcggt ttgcggagtc agcgatgcaa caaatgccgc
    20161 cagcatcgac ggcacaaagt gcgtggtcgt tacaccatat tcggcaaaga attgctgcat
    20221 agcgagcggg tcgcggtgcg cttccggttc agccatcacc agtttagccc cggcaataaa
    20281 cggccagaaa aactcccaca ccgagacatc aaaactgcac ggcgtttttt gggcaacgac
    20341 atcttcgcct gtaagcgggt agtggttttg catccaaagc agacggttaa cgatagccgt
    20401 ctgcccgacc attacccctt tcggcctgcc ggtggagcca gaggtaaaga tgatataagc
    20461 cgtgtggtgc ggttgtgaaa gtttcagcgg cgcactgccc tgcggtgtaa gcggggcgtt
    20521 atagcaaagg ctggttaaat ctggaacatc ggcaaagcgc ggcagttgat cgtcggtggt
    20581 gattaacagc gacggacgcg catcttccag catcattttc aggcgatcgt ccggatagcc
    20641 ggtatccagc ggtaaccagg ccgcaccagc ctcaacaatt gcatgtagcg ccagagtcaa
    20701 aaagaccgag cgcggtagcg ccaccgccac gctgtcgcct ggtttaacgc cgcgctcacg
    20761 cagcagattc gccagcgcca ccacctgctc gcgcatttcc cgatagctga acaggtaacg
    20821 cgcatctgcc agcgccggag catgcggtgt ttttgccgct tgttctgcca ccagcgcgct
    20881 cagcgtggtt tctggaatct caacctgagt ggcgttgatc tgcgccagct gcgcatattc
    20941 acctggcagc ataatatcga catcgccgca caacagcgca ggatccgcgg cgaactgggc
    21001 aatcagcatt ttcaggcgtt cagcatgctg gattaacgtt ggctcagcgt aacgctgttt
    21061 attggcgagg atttcaatac tcaaatctcc gtgttcatcc gggaacaggg ccagttcaag
    21121 gtcattaacc ggaccggttg ccagggtatg ggtttgcgcc tgaacaccag gaatatccag
    21181 ttggtaatca aataccttga tattgagtac cggaccgaac agcggttcct ctcctgccgc
    21241 tcgcccactg tcacggacaa tttgttcggc gtcgtagcgt tgatggcggc gcattttttt
    21301 cagttgtgct gccagtcggg ttgccagttg cggcagcgtt tcttgcgccg caatgtgaat
    21361 acccaacggt aaaacgttga gcacgggtcc ggtagccgtc agcgccgccg agcccagtcg
    21421 acgcataaag ataaatccgg cagcgtagtc catgcggttg cacaatcgcc ccagccacaa
    21481 agccgtcagc gcaagggcta aatcggtacg ctgcacacct gaaagttgcg tagccagctg
    21541 gcggaattcc ccgtcggtaa attccagttt caggcgcaaa atatctgccg aagcgctgcg
    21601 ccccggtaaa ggtgccggag aaagtgacgc gggcggtggc agttgacgac gctgttccgc
    21661 ccagaatgcc gcatcacgct gccaggcttc gctttcgcgg tattgctggt actcttccac
    21721 tacatcagcg aaaggcgtaa acggcgaagc aggcgttggt tcgccacgca gccatgcgca
    21781 gtaaatattg gcgatctggc gggtaatggc cgggaaactg aagccatcga ccagcaaatg
    21841 gtgataacgc tgataccagt accagcggtt atccgccacc tgtatcaact gatgaaagac
    21901 cagtggttta ccgctatcga cgcgcagatc ttgttgcaaa tccgcctgca ttaatgactg
    21961 cgcagtaccg tgcggatcaa tgttggttcg caggtcgata gtttctggca gttcgaacgt
    22021 cagcgcatca tcgacccact gccagacttc gccgttatct tccgtaaaac gcatccgcag
    22081 cgtatctgct tgcgctaatc cggcaaccac cgcgcgggcc agtaatggcg catcaacctc
    22141 tccggttaac tcaacgtaat gcgccacgct ccaggcggag ggtaaatccg acagtttttc
    22201 tgccatccag atgccgggct gtgcggcgac caaaggtaaa tgctggctca ttgtgcctcc
    22261 tgcaactggg tgaaattcgc cggtgtcaga gtacgccagt gggcttccag ccactgctgg
    22321 caggacacct gtgactgcgg ctgacacaca acgtcccagc ctgccggtaa ggcgcattgt
    22381 tgcggccaca gactgaattg cccctgcgca ttgcgcaaaa tgtaaaacgc tccctgcgga
    22441 tcatcgaagg gattactgaa tgccatattc aactcctgtc atggaaaagt ggttgccaga
    22501 ggtcgattag cccctgcatc aagccaccgc gccaacaaag cgcatcatgt ccgccgtcaa
    22561 cctgacgcca gaaaatggat tcttttatgg ggtgtaattg cgcatacagc gcctgattgg
    22621 cctgcatgat catcggctcg cgaatacccg cttccagcac aatgcgcaga ccttcggcac
    22681 taacttgacc agctttaagt tgctcaagta aaacaccttc ttgctgcccg ccccgatgcg
    22741 gccaccagta cgatcctgac tggcttaata cacagccaaa gcgttcaggc cagtgcagtc
    22801 cggcatacag cgcggaaagc ccaccaaaac tctgcccggc gaccacggtg cgatcggcgc
    22861 gatcgctaaa aggggcaata gctttcacca ggggtaataa ctcttgctgt actgccagcc
    22921 agaaatccgc attacacggc agttcgtggg cgcggtgcgt ggtgtcaata gcgtcgatca
    22981 acacatacac ggctggagga agttgctgac gatgggtcaa cgaagtcagc acaggccaga
    23041 caggcatact ctgcgcccaa aattcgccat cgagcaaaac tgccagcgga cgttctcctg
    23101 ctgttgcatc gccagtggta aaaatccata cacggcgtga atttttcaac cgctcacttt
    23161 tccagataat ttctttggca ggtgtttctg gcgcttgcgg acaatcccat cccggttgta
    23221 ggggcgcttg cggcatttcg agtgcagaaa cagcgtgccc tcgcccgcct ttccagcttt
    23281 gtggattcag cggatcggct atcgcctggg gtaatagttt tcgccagcct tcacgtaatt
    23341 cgaggcgatc gggggatggt gcagaaaaaa tgtcatcgcg ttcggtggga ataaaacagt
    23401 agctgccgcg ccagttggca ttaagttgtg tcgtccactg ccagacgttg gtgcctgcaa
    23461 ttcgctgcat cgactggggc tggctgttct gatggtgatc ggtcacacca gtgatgtaga
    23521 cccatacgcg ctttatcgtc gagtattctt cagaaccctg gggatcacgc caccggaaag
    23581 tgacctcaaa catttcgtca ttcagacgct gccattccgg gccatgtttc gactgccacc
    23641 agctctcact tcctactttt aacgccgtca caccataacc ccatgtttac tgtgcaattt
    23701 ttcattgatt gcagaaatat attgataata ttattgataa ctatttgcat ttgcaatagc
    23761 gtaatggcgc gccgtgggaa gcgcggacat taattaacca actgcactgc gtgtctttca
    23821 ggatcaaagg ttttcgcggt agcgggatgc gtcgtgttga tgacgaccat gcccgacagt
    23881 tgcaattcgt ggcaaaaatg caggaataaa acaatgaaca agaagattca ttccctggcc
    23941 ttgttggtca atctggggat ttatggggta gcgcaggcac aagagccgac cgatactcct
    24001 gtttcacatg acgataccat tgtcgttacc gccgccgagc agaacttgca ggcacctggc
    24061 gtttcgacca tcaccgcaga tgaaatccgc aaaaacccgg ttgcccgcga tgtgtcggag
    24121 atcatccgta ccatgccagg cgttaacctg accggtaact ccaccagtgg tcagcgtggg
    24181 aataaccgcc agattgatat tcgcggtatg ggtccggaaa acacgctgat tttgattgac
    24241 ggcaagccgg taagcagccg taactcggtg cgtcagggct ggcgtggcga gcgcgatacc
    24301 cgtggtgata cctcctgggt gccacctgaa atgattgaac gtattgaagt tctgcgtggt
    24361 ccggcagctg cgcgttatgg caacggcgcg gcgggcggcg tggttaacat cattaccaaa
    24421 aaaggcagtg gtgagtagca cggctcgtgg gacgcttatt tcaatgcgcc agaacataaa
    24481 gaggaaggtg ccaccaaacg cactaacttt agcctcaccg gtccgctggg cgacgaattc
    24541 agcttccgct tgtatggcaa cctcgacaaa actcaggctg acgcgtggga tatcaaccag
    24601 ggccatcagt ccgcgcgtgc cggaacgtat gccacgacgt taccagccgg gcgcgaaggg
    24661 gtgatcaaca aagatattaa cggcgtggtg cgctgggact tcgcaccatt gcaatcgctg
    24721 gaactggaag cgggttacag ccgccagggt aacctgtatg cgggcgatac ccagaacacc
    24781 aactctgact cctatacccg ctcgaaatat ggcgatgaaa ccaaccgtct gtatcgccag
    24841 aactactcgc tgacatggaa cggcggctgg gataacggcg tgaccaccag caactgggtg
    24901 cagtacgaac acacccgtaa ctcgcgtatt ccggaaggtc tggcgggcgg taccgaaggg
    24961 aaatttaacg aaaaagcgac acaggatttc gtcgatatcg atctcgatga cgtgatgctg
    25021 cacagcgaag ttaacctgcc gattgatttc ctcgttaacc agacgctgac gctgggtacg
    25081 gagtggaatc agcagcggat gaaggacttg agttccaaca cccaagcgct caccgggacg
    25141 aataccggcg gcgctattga tggcgtgagt gccaccgacc gtagcccgta ttcaaaagca
    25201 gaaattttct cgctgtttgc cgaaaacaat atggagctaa ctgacagcac catcgtaacg
    25261 ccggggctgc gtttcgatca tcacagtatt gtcggcaata actggagtcc ggcgctgaac
    25321 atatcgcaag gtttaggcga tgacttcacg ctgaaaatgg gcattgctcg cgcttataaa
    25381 gcgccgagtc tgtaccagac taacccaaac tacattctct acagtaaagg tcagggctgc
    25441 tatgccagcg cgggcggctg ctatctgcaa ggtaatgatg acctgaaagc ggaaaccagc
    25501 atcaacaaag agattggtct ggagttcaaa cgcgacggtt ggctggcggg cgtcacctgg
    25561 ttccgtaacg attatcgcaa taagattgaa gcgggctatg tggctgtagg gcaaaacgca
    25621 gtcggcaccg atctctatca gtgggataac gtaccgaaag cggtggttga aggtctggaa
    25681 ggatcgttaa acgtaccggt tagcgaaacg gtgatgtgga ccaataacat cacttatatg
    25741 ctgaagagtg aaaacaaaac cacgggcgac cgtttgtcga tcatcccgga gtatacgttg
    25801 aactcaacgc tgagctggca ggcacgggaa gatttgtcga tgcaaacaac cttcacctgg
    25861 tacggcaagc agcagccgaa gaagtacaac tataaaggtc agccagcggt tggaccggaa
    25921 accaaagaaa tcagtcctta cagcattgtg ggtctgagcg cgacctggga tgtgacgaag
    25981 aatgtcagtc tgaccggcgg cgtggacaac ctgttcgaca aacgtttgtg gcgtgcgggt
    26041 aatgcccaga ccacgggcga tctggcagga gccaactata tcgccggtgc cggggcgtat
    26101 acctataacg agccgggacg tacgtggtat atgagcatta atactcactt ctgatgctaa
    26161 cgtcagattg ttgacaaagt gcgcgtaatt catgccggat gcggcgtgaa actgaactgc
    26221 accccaaatg ttggacataa tctgacatct gaaggtgcag tttatgaaac attcatttga
    26281 agtaaaactt gccgctgtaa atcattacct ggctggtcat gcaggcatca tttctacggc
    26341 aaaactcttt caactttctc acaccagcct ctcacactgg attaatcttt ttcttcttca
    26401 cggtcctcgg gcactggatt gcagacacaa gcgtagctat tctcctgaag ataaactttg
    26461 cgtggttctt tatgctctcg ggcattctga gtctctaccc cgggtagctg cccggtttaa
    26521 cattcccagc cacaacatgg ttaaaaactg gataaaaggc taccgcaaat ctggtaatga
    26581 ggcctttatc aggcgcagga aggagaaaag catgacccgt tctgatgata cccatgaaaa
    26641 cgaggcaaac atgacgccgg aagagatgaa gaatgagctg cgttatcttc gcgcagaaaa
    26701 tgcatatctg aaagccatgc aggagcatct cctggaaaaa aagcgccagg agctggagaa
    26761 aaaacgaaag tcatccggag cctgaagtgc ggacactgcc agtctgacct gttaaaggct
    26821 gcagggctgg ccagaagcac gctgtactac cagctgtcac tgcagaaggc taaggacaag
    26881 tatgctgatg tgaaacagct catagcctcc atcttccatg aacaccgggg atgttatggg
    26941 taccggcgga tccattgtga gcttcagaaa agaggtctta agttcagtgg caaaacggtg
    27001 cgaaaactga tgcaacagct cggcctgaag tctcctgtgc gcctgaagaa ataccggtct
    27061 taccggggaa atatgggact cgctgcagaa aatatccttc agcggcagtt caaagcggaa
    27121 gctccctgtg agaaatgggt gacagatatc accgaattca gggctggcgg acaaaaactg
    27181 tacctgtccc cgatacttga tttgtttaac ggggaaatcg tggcgtggga aactgcctgc
    27241 aggcctacag aggagctggt gaagcgcatg ttaaataagg ggctggaaag ccttgcagaa
    27301 ggagaaaaac cgctgctcca caatgatcaa gggtggcatt accggataaa aagttatcag
    27361 tctgccctgg cagacaaagg gttagtgcag agcatgtcac gcaagggcaa ctgtcttgat
    27421 aatgcggtaa tggagaattt cttaggtcac ctgaaagaag aaatgtacta tcgccgggac
    27481 tacagaagtg tagaagagct ggaaaatgcc gttaacgaat acataactta ctggaatcaa
    27541 aaaagaataa aactcagtct tgggggactc agcccgttag aataccggac tgagtatcaa
    27601 aaagccggtt aactgaaact gtccaggttt tggggttcag ttcagaacgc cttatccggc
    27661 ctacaaaaat cttgccaatt caatatattg caggatagtg taggcctgat aagcgtagcg
    27721 catcaggcag ttttgcgttt gtcatcagtc tctaatatgg tcgatatgaa aactacgcat
    27781 acctccctcc cctttgccgg acatacgctg cattttgttg agttcgagcc agcaagtttt
    27841 cacgaacagg atttactctg gctgccgcat tatgcgcaac tgcaacatgc cggacggaaa
    27901 cgcaaagccg aacatttagc cggacggatt gctgctgttc atgctttacg ggaatttggc
    27961 tttaagtacg ccccccctgt cggtgaacgt cgccagccac tttggcctgc cgggttatac
    28021 ggcagtatta gtcactgcca ggctacggca ttagcggtgg tatcccatca accaacgggt
    28081 attgatatcg aagagatctt ctctgcacaa gcagcggaag agttgaaaga cagcattatg
    28141 acaccagcag aatacgagct gcttgtggtc agcaatttac ccttttctgt ggtgcttaca
    28201 ctggcgtttt cagcgaaaga gagtgcgttt aaggcaagca agttacagac agatcaaggt
    28261 tttctggatt atcagatagt gaactggaat aaacagcgga tcattatttg ttgggagaac
    28321 gagatatttg ctgtgcaatg gcagataaga ggaaaacaac tcataacgct ttgtcaacac
    28381 gattaatgga taacccttac tgtggttagc cagcaaccat tgcttgtagt agtcaagtaa
    28441 tattgaccac cgttttacgc taagaacgtt atctgttctc taattttttc ggtgtcagcc
    28501 caccgttata atggtgagat ctgacgctat tatagttgtt caatatacaa ccactgattt
    28561 gctgccatat atccggcgtc gttagtggac tgatgacccg cgccatgatg gcatccgcac
    28621 cctgtttcct aacctatgtt accaccccga caccgttcag gttgttacag agtcgattat
    28681 gcgatctgat caatcgctga atatcccaaa tcaccaaccg gacggagcag tgccgatcat
    28741 agcaccaata cccagaactg aacgacgcct gatccagaaa acgatccata aaacaaagga
    28801 caaaaaacca cgcccggcgg ctcacagcca tgctgatgct gcatcgtggt gacaccgtca
    28861 gtcatgctgc cagaacactc tgctgtgctc gctcatcgat tggtcgctgg attaactggt
    28921 tcacgctgtc gggtgttgaa ggcctgaagt cattgccgtc aggacgtgag aggcgctggc
    28981 cgttcgagca tatttgcgca ttgctgcgtg agcttgttaa gcattctccc ggcgatggtt
    29041 acctgcagcc gaactggtat ggcgcagagc agcaccaaca ctgcgtatcc ggggcccgaa
    29101 taaggaagaa aaaatggcag cgattaatgt agcgttagtc agttgtagca tagaacaccc
    29161 tgtgttttac gaagatgaag tggagatcca cctcaatccc aaaatcggcg cagactggca
    29221 gttacgcggg cagcaaaagc gcgttgttac gccgggccgg aatgaaaagt actatcttgc
    29281 cggtgcgctg cacagtggta cgggcaaagt cagctacgtg ggtggaaaca gtaaaggttc
    29341 atccttattt atcaggctgt taaaacatct gaaggagaca tactgacggg caaaaaagat
    29401 tacgctcatt gtcgataact acatcaccca caaaagctgt gaaacgttgc gctggctgaa
    29461 agcaaaccca aagttcaggg tgatttatca gccaatttac tcgccatggg tcaatcatgt
    29521 cgaacggttg tggcaggcgc ttcatgaaac aataacccga aatcaccagt gccgttcaat
    29581 gtggcaactg ttgaaaaagg ttcgtcactt tatggatacc gtcagcccat tccccggtgg
    29641 aaagcatggg ctggctaaag tgtagcggta ttaggatcag ctacttagaa gcgaatggag
    29701 taactggcat agcacagggg aatttaacta ctcagcatat atatgcaaga cttgaataat
    29761 atgtgggcgc gtaaatagct cataaaaatt tcaacaacac tccctcgctg tattataaag
    29821 taaccacaaa aaaatgttct tttttggatt tgttataaat atcccagtca atttcccaat
    29881 cattattaaa aacaatagtt ccaccataat tctggacatt tattaacttg gtgtttggtg
    29941 caacggaaaa ctttgcaagt aagctaccgt taaccaatgt agtcttatat cccctaccgt
    30001 tattaagtct tatgtaatca gattgtgtta ttacgacccc gttgt
//
LOCUS       DAZKGF010000042        29938 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZKGF010000042 DAZKGF010000000
VERSION     DAZKGF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.042"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..592)
                     /gene="yeeO"
                     /locus_tag="ACHOQV_001809"
     CDS             complement(<1..592)
                     /gene="yeeO"
                     /locus_tag="ACHOQV_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP9630196.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVVMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLIN"
     gene            753..828
                     /locus_tag="ACHOQV_001810"
     tRNA            753..828
                     /locus_tag="ACHOQV_001810"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:786..788,aa:Asn,seq:gtt)
     gene            complement(866..1816)
                     /gene="cbl"
                     /locus_tag="ACHOQV_001811"
     CDS             complement(866..1816)
                     /gene="cbl"
                     /locus_tag="ACHOQV_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFP9630197.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1918..2835)
                     /gene="nac"
                     /locus_tag="ACHOQV_001812"
     CDS             complement(1918..2835)
                     /gene="nac"
                     /locus_tag="ACHOQV_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFP9630198.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3162..3237
                     /locus_tag="ACHOQV_001813"
     tRNA            3162..3237
                     /locus_tag="ACHOQV_001813"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3195..3197,aa:Asn,seq:gtt)
     gene            complement(3292..4227)
                     /gene="ldtA"
                     /locus_tag="ACHOQV_001814"
     CDS             complement(3292..4227)
                     /gene="ldtA"
                     /locus_tag="ACHOQV_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9630199.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(4289..5368)
                     /gene="cobT"
                     /locus_tag="ACHOQV_001815"
     CDS             complement(4289..5368)
                     /gene="cobT"
                     /locus_tag="ACHOQV_001815"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFP9630200.1"
                     /translation="MQILADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAILVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPISGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPRDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5380..6123)
                     /gene="cobS"
                     /locus_tag="ACHOQV_001816"
     CDS             complement(5380..6123)
                     /gene="cobS"
                     /locus_tag="ACHOQV_001816"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFP9630201.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLVKILVLSELTLRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVVIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6120..6665)
                     /gene="cobU"
                     /locus_tag="ACHOQV_001817"
     CDS             complement(6120..6665)
                     /gene="cobU"
                     /locus_tag="ACHOQV_001817"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFP9630202.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WNYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(6799..7236)
                     /locus_tag="ACHOQV_001818"
     CDS             complement(6799..7236)
                     /locus_tag="ACHOQV_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFP9630203.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLNGGSVQSLSDAYSDYSVLLLIAAESEISVPEWLPHSEGTTVIVIAEHNARLLTGPA
                     GGAVALIHYLNEICHARETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(7454..7629)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(7718..8632)
                     /gene="pocR"
                     /locus_tag="ACHOQV_001819"
     CDS             complement(7718..8632)
                     /gene="pocR"
                     /locus_tag="ACHOQV_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HFP9630204.1"
                     /translation="MISAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFT
                     PFCQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGF
                     VLCGQVRLRNDDVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVE
                     NCLKKQLNFVVIKENKSQSDSVRQTRAPNPHDNKMKKALRYIDAHLSDDLRLEDVASH
                     VYLSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYF
                     CKVFRQTYQVTPQVYRQQVSENAAMETV"
     gene            complement(8835..9644)
                     /gene="pduF"
                     /locus_tag="ACHOQV_001820"
     CDS             complement(8835..9644)
                     /gene="pduF"
                     /locus_tag="ACHOQV_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFP9630205.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVTGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNIVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWFAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKEISE"
     gene            10157..10441
                     /gene="pduA"
                     /locus_tag="ACHOQV_001821"
     CDS             10157..10441
                     /gene="pduA"
                     /locus_tag="ACHOQV_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFP9630206.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            10438..11250
                     /gene="pduB"
                     /locus_tag="ACHOQV_001822"
     CDS             10438..11250
                     /gene="pduB"
                     /locus_tag="ACHOQV_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFP9630207.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            11269..12933
                     /gene="pduC"
                     /locus_tag="ACHOQV_001823"
     CDS             11269..12933
                     /gene="pduC"
                     /locus_tag="ACHOQV_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFP9630208.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPANQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNIKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            12943..13611
                     /locus_tag="ACHOQV_001824"
     CDS             12943..13611
                     /locus_tag="ACHOQV_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFP9630209.1"
                     /translation="MEINEKLLRQIIEDVLAEMQTSDKPVSFRAPVSATASSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            13626..14144
                     /gene="pduE"
                     /locus_tag="ACHOQV_001825"
     CDS             13626..14144
                     /gene="pduE"
                     /locus_tag="ACHOQV_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFP9630210.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASIQSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTAQDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLERRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            14154..15986
                     /locus_tag="ACHOQV_001826"
     CDS             14154..15986
                     /locus_tag="ACHOQV_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFP9630211.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSYVSSALAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGSNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVNVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQKGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     LGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKGAIHGK"
     gene            15976..16326
                     /locus_tag="ACHOQV_001827"
     CDS             15976..16326
                     /locus_tag="ACHOQV_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFP9630212.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDQEKLIVHYKNLPASAPLFTLMYQQDNHARRSIGTNA
                     ARLVKGIPFRELHS"
     gene            16346..16621
                     /gene="pduJ"
                     /locus_tag="ACHOQV_001828"
     CDS             16346..16621
                     /gene="pduJ"
                     /locus_tag="ACHOQV_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFP9630213.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            16645..17067
                     /locus_tag="ACHOQV_001829"
     CDS             16645..17067
                     /locus_tag="ACHOQV_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFP9630214.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSETP
                     ATTVISEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            17070..17702
                     /locus_tag="ACHOQV_001830"
     CDS             17070..17702
                     /locus_tag="ACHOQV_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFP9630215.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKPLMQPGQFAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVTVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVT"
     gene            17699..18190
                     /gene="pduM"
                     /locus_tag="ACHOQV_001831"
     CDS             17699..18190
                     /gene="pduM"
                     /locus_tag="ACHOQV_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFP9630216.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRAAIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHDRLLSYVDIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            18194..18469
                     /locus_tag="ACHOQV_001832"
     CDS             18194..18469
                     /locus_tag="ACHOQV_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HFP9630217.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRQVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            18481..19488
                     /gene="pduO"
                     /locus_tag="ACHOQV_001833"
     CDS             18481..19488
                     /gene="pduO"
                     /locus_tag="ACHOQV_001833"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFP9630218.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHCFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPGA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            19485..20870
                     /gene="pduP"
                     /locus_tag="ACHOQV_001834"
     CDS             19485..20870
                     /gene="pduP"
                     /locus_tag="ACHOQV_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HFP9630219.1"
                     /translation="MNNSELETLIRNILREQLVPATQRAQRNAIFQTVDEAVCAAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESAKETGMGNKEDKYLKNKAALDNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKNVSLKLITIIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKISVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VVSVAEYLIQQMQSFGALLLNHEQTEKLRAICLPDGSANKKLVGKSPSALLEAAGLPL
                     PAKAPRLLIAVVDADDSWVTCEQLMPMLPIVKVNDFDSALALALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            20881..21993
                     /locus_tag="ACHOQV_001835"
     CDS             20881..21993
                     /locus_tag="ACHOQV_001835"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFP9630220.1"
                     /translation="MKTFSLQTRLYSGTGSLNILNRFTNRHIWIICDVFLAHSPLIDT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGIAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIHFNAGD
                     PRAAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLKTTCTLPTLTNALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            21990..23333
                     /locus_tag="ACHOQV_001836"
     CDS             21990..23333
                     /locus_tag="ACHOQV_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9630221.1"
                     /translation="MNVELTSCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMVQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVNVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTINGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGNLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLTVAKTVCEQCRLCTDLCPRHLVG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLIPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            23336..23890
                     /gene="pduT"
                     /locus_tag="ACHOQV_001837"
     CDS             23336..23890
                     /gene="pduT"
                     /locus_tag="ACHOQV_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFP9630222.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            23890..24240
                     /gene="pduU"
                     /locus_tag="ACHOQV_001838"
     CDS             23890..24240
                     /gene="pduU"
                     /locus_tag="ACHOQV_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFP9630223.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTVS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            24245..24688
                     /locus_tag="ACHOQV_001839"
     CDS             24245..24688
                     /locus_tag="ACHOQV_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFP9630224.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     SMEQISLVKCWLREAGAHNVLVTSAVNNNGVTELFALLHTEEGCR"
     gene            complement(24789..25118)
                     /gene="tmaR"
                     /locus_tag="ACHOQV_001840"
     CDS             complement(24789..25118)
                     /gene="tmaR"
                     /locus_tag="ACHOQV_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFP9630225.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(25290..26348)
                     /gene="yeeA"
                     /locus_tag="ACHOQV_001841"
     CDS             complement(25290..26348)
                     /gene="yeeA"
                     /locus_tag="ACHOQV_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9630226.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(26546..27019)
                     /gene="sbmC"
                     /locus_tag="ACHOQV_001842"
     CDS             complement(26546..27019)
                     /gene="sbmC"
                     /locus_tag="ACHOQV_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFP9630227.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(27138..28304)
                     /gene="dacD"
                     /locus_tag="ACHOQV_001843"
     CDS             complement(27138..28304)
                     /gene="dacD"
                     /locus_tag="ACHOQV_001843"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFP9630228.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVAMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            28513..>29938
                     /gene="sbcB"
                     /locus_tag="ACHOQV_001844"
     CDS             28513..>29938
                     /gene="sbcB"
                     /locus_tag="ACHOQV_001844"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFP9630229.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSKFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
BASE COUNT         7903 a         7783 c         7123 g         7129 t
ORIGIN      
        1 cgttaatcag tagcggtatt ttcgtattcc ctgcaccacg aagtgccccg ctaccaataa
       61 gagtgatggc agctgctgga taactgagta cggtcagctc cagataagtc aacgccagtg
      121 ctttaacttc tgtcgtggca tcacccgcga cgaaatcaat aatttgttcg ccaaaatgat
      181 gaattagcgt tgccaacagt acggcaaaca acgtcatgat caccaatgac tgccgcgtcg
      241 ccaccctcgc tcgtcgtcga tctcgcttac cgagactaaa tgctacaacg acagtagtac
      301 caagatcgat agctgcaaaa aaagccataa cgaccatatt gaagctgtcc gccaacccca
      361 cgccggccat cgcatctttt cccagccagc tgaccagaaa agtgctcagg acccccatca
      421 acaggacaca ggcgttctcc atgaagatag gaacagcaag cggggttatc tcgcgccaga
      481 acaacacttt gtagctcttg cgtttagagt gccagcgagt gccgtgaaca acctggcgta
      541 aagcagagga gatattcaaa gccgacctta attgcagaaa gtgaaaccac atttcaaatg
      601 atgagggaga atcagcaaag ctgcaaagat tttcgccaac aaattgtctg caaatgcaac
      661 aaactgttga tagaaacggc aaacagttgg gaaatttaaa aatcgggttt gacaaaatat
      721 ttttcgccgt taagatgtgc ctcaacagcg attcctctgt agttcagtcg gtagaacggc
      781 ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa tttctgaaaa
      841 gcccgctttt atagcgggct ttttgctata tctgataatc aatttcctct tcactgtttt
      901 ccatcacctg gcgcttgata tcctcaactg acagtcctgc attacaaagt tccagaaagc
      961 gccagacata gttacgctga agttgccctc gcttcagtcc caaccagaca gtattagcat
     1021 cgaaaagatg ccgcgtatcc aggcggatta aattcttttc ctcttgttcg ccgctggatt
     1081 gttcggcaac taatccgatc ccaagcccaa gagcaacata ggttttaatg acatcagaat
     1141 cctgtgcact taatacaata tctgccggca aacctttgcg ggcaaatgcg tcatcaatgc
     1201 gtgagcgccc cgtaattccc tgtcggtaag taattaacgg ccacttcgct attgattcca
     1261 gcgtcaatgg tgtaatttgc gttaagggat gatcgagtgg aacaagcaaa ctatggtgcc
     1321 agcgaaacca cgggaaggcg acgagctgcg ggtcattact caaacgctcg ctggcgatac
     1381 caatatcagc ttcgccattt tgcaacaacg tcgcaatttc ctgtggcgta ccctggatta
     1441 gctcgagccg aacctccggg aaaagttcgc gaaaagcttt aatgacctct ggcaagctat
     1501 aacgtgcctg agtatgcgtc gttgcaatag tgagaacacc agacgtatcg ttggtaaaca
     1561 ggtctgcaag ccgacgaaca ttactggctt cattcagaat acgttctgca atgaccagta
     1621 atgctttgcc cggttcagtc atgcccagca gtcgcttacc tcgtcgaaca aatatttcga
     1681 tgccaagttc atcctccagt tcccgaatat gacggctaac gcctgactgt gaggtaaaaa
     1741 gcatattcgc aacctctgtc aggttgtaat cctgacgtgc agcctcgcgg attatcttta
     1801 gttgttggaa attcacggta aactccgggc agttcagata tcccgttatt gttaaagtct
     1861 aatgcccggc ataacaaata ataaaaaccc gcatcttatt ccatcccgat ataacgctta
     1921 gctcaccaat tgccactgcc ttttttccat cactggagaa ctaatcactg acattaacaa
     1981 ctctttcact gcctgtgcct gtggtgacaa gtttgctctg gcgggtaaat ttaaggacaa
     2041 agagagactc attgaaggag tggtaatgcg tgacatccac ccatttactg cgccacataa
     2101 cgaacgcgca gccgattcag gtaatactgc aacgcccatg ccgctggcaa tcgctgcggt
     2161 aagcgtggca atagactcaa tttcaccaat gacttttgcc gtgagccgcc gtagggaaaa
     2221 agcctcatca acacgaagtc taacagcgct gtaatcactg gggagaaaaa ggttcatttg
     2281 cgcaatagca ttaacatcaa cgctttgccc cgggcaatct tgagttccta ccaggaaaag
     2341 atcttctttc agcaaagcct gactggatac cccagccaca ggggaatgct cataaatcac
     2401 cgccatatcg agttggtgat ttatcagttt ttcgttaagc actgcaccac tattttcatg
     2461 aagatagata acgacctccg ggaattcagc gcgaaccgcc tgtaataagg gcatggtgat
     2521 ggatgacgca gcggttcctg gcgcaaagcc aatcgagact tgccccgata atgactgacc
     2581 aacgttatgc accgccagtt gggcctgctc acactgacgt aaaatggccc gtgcatgggt
     2641 atagagaatt tttccggcgt ctgttggcgt aacgccccgt tttgtgcgaa tcaaaagctg
     2701 ttgatttaac tcaccttcca gtgtggcaac ctgctggctg agcgctggtt gtgcgatatg
     2761 caatacttca gcagcctggg tcaggctacc aatatctaca atttttacga agtatttcag
     2821 gcgtctgaag ttcatgttgc ctccggtttt taagaatcgg cccaagtgcc gccattactt
     2881 acaaccagaa tgcaagatgc ttgccagttc tattttagcg ttgatgcgca agctaaccaa
     2941 ctgtcaaata agagattatg atagattcgt cattggccct ttttatcagc agtcgcattc
     3001 acctgcccta taaaaggagg gtatgcacca cgatggttca ttacccaata agattgaaag
     3061 ctcaccactt tgttgaaatt ggcagcaaac aaacaaaaaa atgcatttca ccctttgaca
     3121 tcaccatgca ctgccattaa tatgcgcccc gttcacacga ttcctctgta gttcagtcgg
     3181 tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat
     3241 tcaaaaaagc ctgcttcgag caggcttttt gctttctaat taccaacgct cttaaaacat
     3301 ctgtcttgaa ccagaactaa tttgcacagg cattcccgat cgatgttgca acgcagcatt
     3361 tgcgcgattt acatcaactt cttgcccgtt gataaacgcc cgcaaagatg gggttaccgg
     3421 caatggcact tttcggtcag actcatattc tgcacgattg cgcgacagcg gctcatgaac
     3481 ttccagccac tttgaaccat ccggttccgt tgtgtatttc actggctgat caataatttg
     3541 tacacgagtg cctacaggaa cattatcaaa cagatatttg atatcgtcat tgcgcagacg
     3601 aatacagccc tgacttaccc ggagcccaat accaaaattg gcattggtac cgtggatggc
     3661 atacaacctg ccaatataaa tcgcgtacag ccccatgggg ttatcgggcc ccgcaggaac
     3721 aaatgcgggc aaactctccc ctcgtttcgc atattcgcgc cgagtgttcg gcgttggcgt
     3781 ccaggttggt gcttcttgtt tacgttcaac ggtagtcacc cagttacgcg gggtttctcg
     3841 cccagcctgg ccgataccaa taggaaagac ttccactgta ttactgtctg gcggatagta
     3901 ataaagacgc atttcagcga cgttaaccac aatcccttta cggacagtgg cgggcaaaat
     3961 cagttgctgc ggaatggtga gttgtgagcc agacttcggc aaaaaaacat cagcgcccgg
     4021 attcgcttcc agcatattac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg
     4081 ctgagtattg tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc
     4141 tggaggtaat ggataagtta ccgccaggct attttggctg gcaaaaagca gagcaaatga
     4201 gcaaagaata tttacacgac gcatcatgtc cctttcctaa gtcgcgaata ctatccgata
     4261 agtatagctt ttatcagact tttcgttttt agctgttcaa atcagaagtc gtattccccg
     4321 gtagaacaat attactggca gcaagttcgc ccatattgtt gtatatcgca caagcagctt
     4381 cgatgatggg catcgccaga gctgcaccac tcccctcacc taaacgcata tccatattga
     4441 gataaggctc cagccccaaa tgcgacagcg ctatacgcgc gcctttttct gccgacaagt
     4501 gagaaggaat gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcagcag
     4561 cataagaaag aaatccatcc agcaacacgg gtaaaccgca ggaagcagca cctaacatca
     4621 ctccagctat tccgaccaaa tcaaatccac ccacttttgc caggacatca acaccatccc
     4681 gaggatttgg ttgattcaac gtaatcgccc gacgcacaac atcaatttta ttagccagtt
     4741 tatctgtcgg caggtttgcg ccaatcccaa ccacttcttc aggatcccgg ccagtgattg
     4801 tgctgactat tgccgctgcc ggtgtcgtgt ttgccatccc cagttcacct acaccaaaca
     4861 gcgtgacacc gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt
     4921 cagcctgacg gcgactcatt gccggagctg aagcaatatt gccgctacct cgtgcgacac
     4981 gcatgttgat aagcccggag ataggctcag cagtatcaat accaacatca attacgtgga
     5041 cgttagcgcc cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt
     5101 cagcctgtat ggctgtcact tcttttggag aaatagcgac cccttcctcc cagacgccgt
     5161 gatcggcaca cataaccagt atcgcttttt tgcccacatg cggtatgcca ttcaaccccg
     5221 gcattcctgc cagttgtatg gcaagcgcct ccagctttcc caggctacca acaggtttga
     5281 gtaacccgtc aatatgccgt tgtgcacgcg acatagcggc aggatcgatg gctgggatcg
     5341 tattcagtaa atcggcaagt atttgcatgt cacgtctctt tatagcagtg ccagcaggaa
     5401 aaccagttca ccaagctcta tggctgctcc cagcgtatcg cccgtttgcc cgcccagcgt
     5461 acgtttgaga agctgaccga ggatgaaaat caccaccatc gtcaccacca tagcagccac
     5521 accatgcata cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca
     5581 ggtttgtcgc ccatcaattt tgccaatgaa cacattgcca agcccttctt cccgcgcata
     5641 acgatgacga tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag
     5701 cgatgcaagg atcggttcgc cacgcagagt caactcgctt aacaccagaa tctttaccag
     5761 taccacaaaa atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc
     5821 caacatgcga tcgcggctac gtgcagaaaa tacgccatca caggtatcag cgaggccgtc
     5881 cagatggaat cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg
     5941 tacgccacac catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa
     6001 tccaatcaaa ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga
     6061 ccagcgacgc ggtacaggca gacgcgtaat aaatgagagc atcgcccaaa ataatttact
     6121 catttgattt ttactccaat acccgaaacc accagccata cttcatttgc cgcagcggcc
     6181 aactgctgat ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc
     6241 acaatcccca ttcccacttc gttagtcact aatacaacct ttgcggggca acgttggcag
     6301 gcagcaatca acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataattccat
     6361 tcatcagggt ctttatcgcc gccataatca aacaacagat tagtcaccat tgtggtaacg
     6421 cattccagca acacagcctc attcgggtta atgtctgcat gaattaattc atcaagatgt
     6481 tgccaacgct ccactgtgcg ccagtgctcc gggcggcctt gccgatgatg ttctatccgt
     6541 gcagccatct catcatcaag gatttgcgag gtggcgatat acagaacctg tgaagagtcc
     6601 ccaataagcg cctctgcgtg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc
     6661 atcataccga ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc
     6721 ccaccgttgc tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa
     6781 tccagcattc tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg
     6841 tgttaacgtc cgacaaaatg cgtcattgac agcggtttct ctggcatgac agatttcatt
     6901 cagataatgg atcaatgcca cagccccacc agccggacca gtcagcaaac gggcattgtg
     6961 ttctgcaatc acaattaccg tcgttccctc agaatgaggg agccattctg gtacggatat
     7021 ctcgctttca gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataatga
     7081 ttgcacagac ccaccgttaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca
     7141 ggttattggc aatatttcag ccagatgttt cgccaggtcc acatctccag ataagaggca
     7201 aaaaagagct atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttagtttc
     7261 ctgtaatgtc ggtctggttg atgagccatg aaacaagatt gccacacctt aagtgcagcc
     7321 ctggtgcctg tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt
     7381 cagggtggta ggggtataaa gacgcgccgg tactctaccg gacgcttccg ggcaacccac
     7441 ttcccaccga agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc
     7501 ccccgacctt cccaatctcg cgatcagtgg tattttcggg tttgtcagca ttacagcagc
     7561 gggggctgcg agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt
     7621 atgttatgag ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca
     7681 gcgtttattt aaaccttccc tttttttagc atttactcta taccgtttcc atcgccgcat
     7741 tttcactcac ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt
     7801 tgcaaaaata actggtttgc gaaaaaccta aattacgcgc aatgctggcg atgctccagt
     7861 cactatggca cagtaactca cgagcgctgg ccatccgttg ttggtttacc cacgcattaa
     7921 aaccgattcc atggtatttc ttgaataact tactgaaata ataaggactt agataaacgt
     7981 gcgaggcgac atcttccaga cgcaaatcgt ctgataaatg cgcatcaata tagcgtaaag
     8041 cttttttcat tttattgtcg tggggattag gcgcacgagt ctggcgcacc gaatctgact
     8101 gagatttgtt ctctttaata accacaaaat tgagttgttt tttgaggcaa ttttccacaa
     8161 tcaacttcaa caaatcagca gaggccataa ctcttgagta atccatctca ggaactttgc
     8221 ggaattcatt aagtaattcg ggatctgcct gccagcaatc atcgacattc agaatatcga
     8281 ccaaatcgac atcatcatta cgcagacgaa cttgcccgca caatacaaaa ccaactaaat
     8341 gtccggcgat taccagtgga atagagaaat cggttaatcc ggcatgacag cgataaatac
     8401 aaggctgatc ggatttagag gcttccagac ccccacaacg atcgctcatg cgacatcgag
     8461 tgctcagctg agggtgttgc cgcatcatct gacaaaatgg agtgaagtta aacagctcgg
     8521 aaatttcatc gccgtgaata ttgaccacga caaccgcaag gctggtcgcc tgcgcaaaat
     8581 cctgcgcgat tttattaatg agttctgaat taagtgtcgc cgcagaaatc atgataaaac
     8641 ctcccagtta atttattgtt atagcaaaac aattgtttta catttaatct tcacatcatc
     8701 cttgatgatt tactgacgag aagcacaaag gacaataaca aaagcctccg tgaagcggga
     8761 gactttttaa taagttcgga aagcagatca caaacagtaa aaaaataaac gtttataact
     8821 gccagattat ttcgttattc gctgatttct ttaggcgtta cactattgac aggtaaattc
     8881 tttgcaatga gatagcgata aatcgccgca ccaacacatg ccccaatgat cggtgcaata
     8941 atgggtacaa tgaaataagg aatattgcgt ccaccagtca tcgcaatttc cccccaaccc
     9001 gcaaaccagg tgaaaatttt aggtccaaaa tcacgcgctg gattcatggc aaaaccggtt
     9061 aatggtccgg tagaagcacc aataacagca accagaatac caatgagtaa aggcgcaagc
     9121 ggtcctttgg gtacgccatt accgtcatcg gtcaacgcca taatcaaccc cattaacatg
     9181 gacgttatga ccacttcgac caacgctgct tgccatacat taatcgccgc tgctggataa
     9241 gtactgaaga tactggcgag ttgtaaactt tctgcgctac cacgcactat attgtgagca
     9301 gattcaaaat cggtaaacaa actgctgtag agagaatatg ccagaaccgc accaccaaag
     9361 gcacccgcta attgcgccac acaaaatggc agcactttac gggctgggaa agaggcaaac
     9421 agccacagag caatggtaac tgccgggttg agatgagcgc cggaaatacc tgccgtaagg
     9481 taaacagcaa gcgaaatccc taatccccaa atgatacaaa tttcccacag tcccagactt
     9541 gccccggtaa ccttcagggc gcacaaacaa ccaatgccaa aaaagaggaa cagtccggtg
     9601 cccaaaaact cggcaacgca ttgcgccttg agtgaatcat tcatagtgac accttcttta
     9661 cggagttttt ctttatcagc caactatgcg ctgattgttg tgtataccga gcgcaactat
     9721 aaaaaattgc tctgcagaaa atattgccaa catgcatgac agactcgtgt aaaaatcgtg
     9781 ctaacataac aaattattgt cattgccgaa tcatgcaaca cagcgttaat cagtgttgaa
     9841 agtaataatt tccaccaaac ttttacaata aaaaattgcc atcatgacat aataaaattg
     9901 aaacaaatat cacagttcct tccttcgaac caaactcaac gaaatattac tcgcgattaa
     9961 actcactagc caacgcaatt aattcacatt gaaatatcta caaaacaacc aataaattaa
    10021 caacatatag ttcataaaag caattttatt atatttgttc tctattaatt tttctcatta
    10081 gccgattaag tcagccattc tttttcctcg ctttttttta tagtcatatc gtcaaaaaaa
    10141 tccaggcgag gtctttatgc aacaagaagc gttaggaatg gtagaaacca aaggcttaac
    10201 tgccgccata gaggccgcag acgccatggt gaagtcggca aatgttttgc tggtcggcta
    10261 cgaaaaaata ggttccgggc tggtgaccgt catcgttcgt ggtgatgtcg gggcggtaaa
    10321 agcggcaaca gacgcgggtg ccgcagctgc gcgtaacgtc ggcgaagtaa aagccgtaca
    10381 cgttattccc cgcccacata ccgatgtcga aaaaatctta ccaaagggaa ttaacccatg
    10441 agcagcaatg aactggtcga acagatcatg gcgcaggtca tcgctcgcgt agcgacgcca
    10501 gagcatgagg ttctcccgaa aaccactcat caaatacgag agacggctat ggcagaaaaa
    10561 agctgcacgt taacagaatt tgttggcacc gctattggcg acactgtagg tctggtcatt
    10621 gcgaacgttg atagcgccct gttggacgcg atgaaacttg aaaaacgcta tcgttccatt
    10681 ggcattctgg gggcgcgtat tggcgcaggg ccacacatta tggccgctga tgaagcggta
    10741 aaagccacca acactgaagt tattagcatt gaacttcctc gggatactaa aggcggtgcc
    10801 ggacacggct cattgatcct cttaggtggc aacgatgttt ccgacgtgaa acgcggtatt
    10861 gaagttgccc tgaaagaact cgatcggacc tttggtgatg tctatgccaa cgaagccggg
    10921 catcttgaac tgcaatacac cgcccgcgcc agctatgcac tggaaaaagc gtttggcgct
    10981 ccactgggac gggcttgcgg cgttattgtc ggcgctccgg ctgcggtggg tgttctgatg
    11041 gccgacaccg cgctgaagtc cgccaatgtg gatgtcgtgg catacagctc ccccgctcaa
    11101 ggcaccagct acagcaacga agtcattctg attatctcgg gtgactccgg cgcagtccgc
    11161 caggcggtca tctccgcacg cgaaatcggt aaaaccgtgc tttcgactct cggcgataca
    11221 ccgaaaaacg atcgcccttc ctacatctga attctctgag gcagatttat gagatcgaaa
    11281 agatttgaag cactggcaaa acgcccggtc aaccaggacg gtttcgttaa agagtggatt
    11341 gaagaaggct tcattgcgat ggaaagcccg aacgatccta aaccgtcaat caaaattgtt
    11401 aacggcgtcg ttaccgaact ggatggaaaa cccgccaatc agtttgacct gatcgaccac
    11461 tttatcgccc gctacggcat caatctcgcg cgcgcagaag aagtcatcgc gatggattcg
    11521 gtaaaacttg ccaatatgtt gtgcgatcca aatgtcaaac gtagcgatat cgttcccctt
    11581 accacagcaa tgacgcctgc aaaaatcgtc gaagtggttt cgcagatgaa cgtggtagag
    11641 atgatgatgg cgatgcagaa aatgcgcgcc cgtcgaacac catcacaaca ggctcacgtc
    11701 accaacatca aagataaccc ggtacaaatt gccgccgacg cagctgaagg cgcatggcgc
    11761 gggtttgatg aacaggaaac caccgttgcc gttgctcgct atgcaccgtt caacgccatc
    11821 gccctgctgg tcggctcaca ggtgggacgt ccgggcgtct tgacccagtg ttcactggaa
    11881 gaagccaccg aactgaaact cggtatgtta ggccacacct gttacgccga aacaatttct
    11941 gtctatggta cagaacctgt tttcaccgat ggcgatgata ccccgtggtc aaaagggttt
    12001 ctcgcgtcat cctatgcgtc tcgcggctta aaaatgcgct ttacttccgg ctccggctct
    12061 gaagtacaga tgggttacgc ggaaggcaaa tcaatgcttt atctcgaggc tcgatgcatt
    12121 tacatcacca aagccgcagg cgttcagggt ctgcagaatg gttcggtaag ctgtatcggt
    12181 gttccatcag ctgtgccatc aggcatccgc gcagtattag cggaaaactt aatttgctca
    12241 tcgctggatc tggaatgcgc ctccagtaat gaccagacct ttacccactc ggatatgcgc
    12301 cgtaccgcac gtttcctgat gcaatttctg ccaggtaccg actttatctc ttccggtttt
    12361 tccgctgtgc cgaactacga caacatgttt gctggttcaa acgaagatgc tgaagacttc
    12421 gatgattaca acgtcattca gcgtgacctg aaggttgatg gcggcctgcg accagtacgc
    12481 gaagaagatg ttatcgctat ccgcaataaa gccgctcgcg ctttacaggc tgtctttgcc
    12541 ggtatgggac taccgcctat taccgatgaa gaagtcgaag cggcaaccta tgcccacggt
    12601 tcaaaagata tgcctgagcg caatattgta gaagacatca agttcgccca ggagattatc
    12661 aataaaaacc ggaacagcct ggaagtggtg aaagccctgg cgcaaggcgg ttttaccgat
    12721 gtcgcccagg acatgctcaa catgcaaaaa gcaaaactga ccggcgatta tctccatacc
    12781 tcagccatca tcgtcgatga cggacaagtg ctctctgcgg tcaatgacgt caatgattac
    12841 gccggaccgg ctacaggtta ccgcctgcaa ggggaacgct gggaagagat caaaaatatt
    12901 cccggtgcac ttgatcccaa cgaaattgac taagggtgag aaatggaaat taacgaaaaa
    12961 ttactgcgtc agattatcga agacgtgctg gcagaaatgc aaaccagcga caagcccgtt
    13021 tcatttcgcg cacctgtctc tgcaactgcc tcttccgcgc cagataccgg aaattttctg
    13081 accgaaattg gcgaagccca acaaggcact cagcaagatg aagtcattat tgctgtaggc
    13141 ccggcgttcg gcctggcgca aaccgtcaat atcatcggta ttccgcataa aaacattttg
    13201 cgcgaagtga ttgccggtat cgaagaagaa ggcattaaag cccgtgtgat tcgctgcttt
    13261 aaatcgtccg atgtcgcatt cgtagcagtc gaaggcgatc gtcttagtgg ttcaggtatt
    13321 gctatcggta ttcagtcgaa aggcacaacg gttatccacc agcagggact tcctcccttg
    13381 tctaacctgg aacttttccc acaagcgcct ttgttgacgc tggatactta tcgtcagatt
    13441 ggcaaaaacg ccgcgcgtta tgcaaagcgt gagtctccac aaccggtgcc gaccctcaac
    13501 gatcaaatgg cgcggccgaa ataccaggcc aaatctgcca ttttgcatat taaagagacg
    13561 aagtacgtgg tcaccggcaa aaaaccgcaa gaactgcgcg tgacgtttta acaaaggaaa
    13621 ccttcatgaa taccgatgca attgagtcga tggtcagaga cgtgttgaac cgcatgaaca
    13681 gtttacagga tgcagctcct gtttctgcgg tccccaacgc gtcaatacag agcgccaaag
    13741 tgacggatta cccgctggca aataaacatc cggaatgggt taaaacggcg accaataaaa
    13801 cgctcgacga tttcactctg gaaaatgtgc taagcgataa cgtcaccgcc caggatatgc
    13861 gtataacccc tgaaacattg cgtattcagg ccgcaattgc cagagatgct ggtcgcgatc
    13921 gcctggcgat gaattttgaa cgcgccgctg agcttacctc agtaccggat gatcgcatcc
    13981 tcgaaatcta caatgcgtta cgcccttatc gttcgactaa gcaagagctt attgccattg
    14041 ctgacgatct tgaacggcgt tatcaggcca aaatttgtgc tgcatttgtc cgtgaagctg
    14101 cggaacttta tgtcgagcgc aaaaaactga aaggcgacga ttaaggacga actatgcgat
    14161 atatagcagg cattgatatc ggcaactcat caacagaggt tgcgctggca acgctcagtg
    14221 caactggaga gttgtcgtac gtgagtagcg ccctggcgga aaccaccgga attaaaggta
    14281 cgttgcgtaa cgtccatggt attcaggagg cgctcgcaca ggcaacgaaa aaagtcggca
    14341 tcaatgttag cgatatttcg cttattcgta tcaacgaagc aactccggtc attggtgatg
    14401 tggcgatgga aaccatcact gaaaccatca tcaccgaatc aaccatgatc ggtcacaacc
    14461 cgaaaactcc gggcggagtt gggctgggcg tagggctcac catcactccg caagagttat
    14521 taacacgccc ggcggacaca ccttacatcc tggtggtgtc atcggcattt gattttgccg
    14581 atatcgcaac gatgataaac gcctccgtac gagccggata tcagctgact ggcgttattc
    14641 tgcaacgcga cgatggcgta ctggtcaata accgcctgga aattcctctc cctattgtgg
    14701 atgaagtcct ctacatcgat cgtattccgc tcggcatgct ggctgcaatt gaagttgccg
    14761 ttcccgggaa agttatcgaa accctgtcta acccgtatgg gattgccacc gttttcgccc
    14821 tcaatgctga agagactaaa aatattgttc ctgtcgcgcg ggcgctaatc ggcaaccgtt
    14881 cagcagtggt agtgaaaacg ccttctggcg acgttaaagc ccgttcgata cccgccggaa
    14941 atatcgaact gctttctgcc gggcgcacaa cgcgggttga tgtagctgct ggtgccgatg
    15001 ccattatgaa agcggtcggt gagtgcccaa aactggaaaa cgtgacggga gaacctggct
    15061 ccaacatcgg cggaatgctg gaacatgttc gccagaccat ggctgaactg accaacaaac
    15121 ccagcaacga aatttttatt caggatttgc tggcgataga tacctctgtc cccgtcaacg
    15181 taaccggtgg tctggcaggt gaattttcac tggaacaggc tgtgggtatt gcctctatgg
    15241 tgaaatccga ccgtctgcaa atggcgatga tcgccagtga aattaagcaa aaactgcatg
    15301 ttgatgttca ggtcggaggc gcagaagcag aagccgccat tcagggggcc ttgactaccc
    15361 ctggaaccac acgtccactg gcaattctgg acttaggtgc aggctctacc gatgcctcca
    15421 tcatcaatca gaaaggggaa atagttgcca ctcaccttgc gggagcgggc gacatggtaa
    15481 cgatgattat cgctcgtgag ctgggcttaa atgatcgtta tctcgcagaa gaaatcaaaa
    15541 agtacccgtt ggcaaaagta gaaagcctgt tccacttacg ccatgaagat ggcagcgttc
    15601 agttcttccc tactccgctg tcccctcatg tatttgcccg cgtttgtgtg gtgaaacctg
    15661 acgaactggt gcctattctc ggtgatttga cgctggaaaa agtacgtgct gtccgtcgta
    15721 gtgcgaaaga gcgtgtcttc gtaactaatg ccctgcgcgc gctacgtcag gtaagccctg
    15781 ccggaaatat tcgcgacatt ccgttcgtcg ttctggtggg tggttcttcg ctggatttcg
    15841 aagtaccgca attggtcacc gacgcgctgg cacattatcg actggtcgcc gggcggggga
    15901 atatccgtgg tagcgaaggt ccgcgcaatg cggtagctac tgggctcctc atcgcctggc
    15961 acaagggggc gattcatgga aagtaaagag cgtgcaccag ccatcgttgt catggagatc
    16021 ggtgactgta tcactacatg ggatgaagtg cttcttggca ttgaagagga aggtattccc
    16081 tttcgcattc aacacattcc gtcaggtgaa gttatcgata gcgcctggct ggcggctcgt
    16141 caatctcccc tgttggttgg cattgcctgc gatcaggaaa aactgattgt gcattacaaa
    16201 aacttaccgg catcagcgcc actgtttacg ctgatgtatc aacaagataa ccatgccagg
    16261 cgcagcatcg gcactaacgc tgcccgcctg gtaaaaggga ttcccttccg ggaactccac
    16321 tcataatcca caggagaaac gcagtatgaa caacgcacta ggactggttg aaacaaaagg
    16381 gttagttggc gcaattgaag ctgcagatgc catggttaaa tccgccaacg tgcagctggt
    16441 tggctatgaa aagattggtt cgggtctgat tacggtgatg gtccgtggcg atgttggcgc
    16501 agtaaaagcc gccgttgatg caggaagcgc cgccgcaagc gccgttggcg aagtgaaatc
    16561 ctgccatgtc atcccgcgcc cacacagtga tgttgaggct attttaccta aatcagcata
    16621 accccattcg ctaaggagca cagcgtgaag caatcactgg gattacttga agtcagtggt
    16681 ctggcactcg ccatcagttg cgcagatgct atggcaaaag ccgcgtccat cacgctggtt
    16741 ggccttgaga agaccaatgg ctcaggctgg acgctcatca aaatcaccgg tgacgtcgcc
    16801 tcggttaatg cggcgatcgc gacgggagcc agtttcgccg ggcagcgaaa tggccttgtc
    16861 gcacacaaag taattgcccg cccgggagac ggcattctgc cccacacaac tgaagcattg
    16921 actgtgacgg ttgaaacaag cgaaacacca gcaacaaccg tcatctcaga gattatcagt
    16981 tgtaatttgt gtcttgaccc ggcatgtcag cggcaaaaag gtgagccccg ctcactttgc
    17041 ctgtacgcca ataagcgagg ggagtagcag tggataaaca aaccctgcag gaaacagtga
    17101 gccagattct ggatgagatg cgcgagcgcc ctatcccact gggcgtttcc agtcgccata
    17161 ttcatctttc aacagcggat tatttgcgtc tttttcctga acaaccgatt agcgagaaaa
    17221 agccgttaat gcaaccgggc caattcgctg ctgaacaggt tgttacgctg gagggaccaa
    17281 aaggcaaatt gcacaacgtt cgcttgttgg ggccgctacg caatgcaagc caggtggaaa
    17341 tttcccggac ggacgccaga acgttaggga tcaccgcccc gctacgcatg tcaggcgatc
    17401 tcagcggcac tccggggatt cgtttgataa gcccatttgc ggaactggag atcacatctg
    17461 gcgtcattgt cgcgcaacgg catattcata tgtctccgct ggatgccctg atttatggtg
    17521 ttacgcacgg cgataaagtg gcggttgcca ttgaaggtga tgagcgacga ctgatattcg
    17581 aaaacgtcac tgttcgcgtc tctccggata tgcgtcttga aatgcatatt gataccgatg
    17641 aagccaacgc cgcaggggct gatagccctg acgtttttgc cacactggtg tcagtgacat
    17701 gaataacatg ctgatgcaac gtattgttga cgaagtggtt tttcgcctta aacaacgtgc
    17761 gggaaaaaca ctcgtgctga ctgtttttca actgcgagac gccagcgttc aagagagcgt
    17821 tcatcagtat gcgtcattac aaatccgata tgtcgatctg ccgctgctgc gacaacttgc
    17881 ggagaacgaa acgtcagatc gagcagcaat ccagatacac gaagcgttag cctggggaat
    17941 gcacattcaa ttgtcgttgc agcgtcattt cctgaacgcc attgaattga agactctcgc
    18001 ccgtctaccg ctaagctggt gcgacgaaca agggcaaccg atctacctac atcatgatcg
    18061 acttttaagt tatgtcgata tcgcacagtt aagctcggga atattggtgt tacagcggaa
    18121 atgttgtgtg accgcactgg ctcgtgaagc ggcaattacc cgaaatattc agttaatcag
    18181 gcaggagtaa cctatgcatc tggctcgagt cactggcgcg gtagtatcca cgcaaaaatc
    18241 accttctctg atcgggaaaa aactgctgct ggttcgccag gtcagtgctg acggcgaact
    18301 gcctctcacg ccattaaccg gcgacgaagt tgccgttgat tccgtcggcg caggcgttgg
    18361 tgagttggta ctcctcagca gtggttccag cgccagacat gtgttttccg gccctaacga
    18421 agccatcgac ctggcagtgg tcggcattgt cgatacgctt tcgcgctaag gacctgaaag
    18481 atggcaattt atacccgaac aggtgattca ggaagtacgt ctttatttac cggacaacgc
    18541 gtcagcaaaa ctcatctacg tgtcgaaacc tatggcacgc tggatgaact gaacgcgaca
    18601 ctcagtctgt gttattgcgc caccgccatc gaaagccatc gcatcttact ggaagccatt
    18661 caacaacaga tattttggtt tagcgccgaa ctcgccagtg aaagcgaaca gccgtcagcg
    18721 cagcagcgtt acatcggtac ggaagaaatt gccgcgctgg aaaacgcgat cgatagcgca
    18781 atgaacgccg tccccccggt tcactgcttc attctgcctg gtcgatgtga agccgcaagc
    18841 cgcatgcatt tcgcccgcac ggtggctcga cgggctgaaa gacgtctggt tgaactgaca
    18901 acagaaacca ccgtccggaa tgttttgctg cactatatca accgactttc cgattgcctg
    18961 tatgccctcg cccgcgtgga agacaacgtt gctcatcaaa acctgatgat tcaggaaatc
    19021 acaaaacgtt atcacgaagc caaccacaca ccagcattga aggaacgcac aatgccgctc
    19081 acttttcagg atcttcacca gctcattcgt agtgcggcaa tgcgcgcaga tgaactgcat
    19141 attcccgttg tcatcagcat tgtcgatgcc aacggaacag aaagtgttac ctggcggatg
    19201 cctgatgccc tgttggtcag cagtgaactg gcaccgaaaa aagcctggac cgccgtggcg
    19261 atgaaaacgg caacccacaa gcttgctgat accgtgcaac ccggcgcgcc gctttatggg
    19321 ctcgaaagcc atatgcaagg caaagtcgtc acctttggtg gcggcttccc cctctggcgt
    19381 gacggaaaat tgcttggcgg gcttggcatc agcggcggta gcgttgaaca agacatggat
    19441 attgcccaaa gcgcaatggc ggcaattaac gtgggagtaa accaatgaat aactctgaat
    19501 tagaaacact gatccgcaat attctgcgtg agcagcttgt ccccgctacg caacgagcgc
    19561 aaagaaacgc tattttccag actgttgatg aagcggtctg cgcagcacac caggcctttt
    19621 tacgctttca acagtgcccg cttaaaaccc gtagcgccat tatcagcgcc attcgcgaag
    19681 aattaatgcc ccgccttacc gaactggcag aagaaagcgc caaagaaacg gggatgggaa
    19741 ataaagaaga taagtaccta aaaaacaaag cggcacttga taacacgcct ggaattgaag
    19801 atttaaccac cacagcactg accggtgatg gaggcatggt tttgtttgaa tactctccgt
    19861 ttggggtgat tggctcggtc acgccaagca ccaaccccac ggaaaccatc attaacaaca
    19921 gcatcagcat gctggcagca ggtaatagcg tctacttcag tccacacccg ggagccaaaa
    19981 acgtttcgtt aaaactgatt accataatcg aagacatcgc gtttcgctgt agtggcattc
    20041 acaacctgat cgttaccgta tctgaaccaa cctttgccgc aaccgaacaa atgatggcgc
    20101 accctaaaat cagcgttctg gcgattaccg gtggcccggg gatcgtcgcc atgggaatga
    20161 aaagtggcaa gaaagtgatt ggtgccggag cgggtaaccc accctgcatt gtcgatgaaa
    20221 cggcggatat tgtgaaagcc gccgaggaca tcatcaacgg cgcttcattt gattacaacc
    20281 tgccgtgtat cgctgaaaaa agcctgattg tcgtggtatc agtggcagag tatttaattc
    20341 agcagatgca aagctttgga gcacttcttc ttaaccatga acaaacggag aaattgcgtg
    20401 caatttgcct gccagacggt agcgccaaca aaaaactggt tgggaaaagc ccatcagcct
    20461 tgctggaagc agcgggcctc cctttgcccg caaaagcgcc gcgcctactg attgctgttg
    20521 ttgacgccga tgatagttgg gttacgtgtg aacaactgat gccaatgctg cccattgtga
    20581 aggtcaacga ttttgacagc gctctcgccc tggcattgaa agtggaagac ggcttgcacc
    20641 acacggcaat tatgcactcg cagaacgtct cacggctcaa ccttgccgcg cgcgttatgc
    20701 aaacctcaat ctttgtcaaa aacggcccct cctgggcggg tattggcgtc ggcggcgaag
    20761 gctttacgac ctttaccatt gcaacaccaa ccggagaggg tacaacgtca gcacggactt
    20821 ttgcccgctc acgacgctgc gtgttaacca gcggcttttc cattcgctag agaaacggaa
    20881 atgaaaactt tttcactgca gacgcgttta tacagcggaa cgggaagtct caacatactg
    20941 aaccgtttca cgaataggca catctggatt atttgcgacg tctttctggc acactcacca
    21001 ctcattgaca cgttgcatca ggcgctgccc gacagtaatc gtctcagtat ttttagcgaa
    21061 ataacgcccg acccaactat ccagaccgtg gtgaaaggca ttgcgcaaat gcagacttta
    21121 cgcccggatg tggttatcgg ttttggcggc ggttctgccc tggacgccgc aaaagcgatt
    21181 gtctggttcg gtcgccagtg tggcattgaa attgaaacct gcgtagccat tcctaccacc
    21241 agcggtaccg gttccgaagt gaccagtgct tgtgtcatca gcgatcctga gaaagggatt
    21301 aaatatcccc ttttcgataa tgcactttat ccggatatcg cgatccttga tccatctttg
    21361 gtcgtcagcg tgccaccggc gataacggcc aataccggga tggatgtttt aacccatgcg
    21421 cttgaagctt atgtttctcc ccgcgccagc gacttcacgg atgcactggt cgaaaaagcc
    21481 gtgcaaattg tttttcaata cctgcccact gcagtgaaga aaggcgattg tcttgccacc
    21541 cgtggcaaaa tgcataatgc atcgacgctt gcaggaatag cctttagcca ggccggactc
    21601 ggtttaaacc atgccattgc tcatcaactc ggcggacagt ttcatctgcc gcacggcctg
    21661 gcgaatgcgc tcctgctcac ggcggttatc cactttaatg ctggcgatcc gcgcgcagcc
    21721 aaacgctacg cccggctggc gaaaacgtgc catttatgcc cggacaacgc caatgacacc
    21781 gccagcctga atgcacttat tcaacatatt gagcagctta aaacaacctg tacgctgccg
    21841 actcttacca atgcgctgaa agaaaaaaaa gcagaatggt caatacgcat accagatatg
    21901 gttcaggcgg cactggcgga tgcaacgttg cgtaccaacc cgcgtgcggc tgatgcctcc
    21961 gctattgcag aactgctcga ggagttgtta tgaacgtcga gttaacctcc tgcgacgacc
    22021 agaccatccg cgagcgagta caggcagcag gtgtcgtggg tgctggcggc gcaggttttc
    22081 caacccatat taagctacaa gcgcgtgttg acacttttct ggtgaatgcg gcggaatgcg
    22141 aaccaatgtt gaaagtggat caacagttaa tggtgcagca agccagccgc ctgcttcgcg
    22201 gtgttcacta tgcgatgaaa gccaccgggg ccagtagcgg aatcatcgcg ctgaaagaga
    22261 aatatcaacg ggcaataaac gcactcaccc ccctattacc accggatatc cgcctgcata
    22321 ttttgcccga cgtttacccc gcaggagacg aagtgctgac catctggatg gcaactggtc
    22381 gccgcgttcc acccgctgca ttacccgtta acgtcggtgt ggtggtcaat aatgttcaaa
    22441 ccgtacttaa tattgccaga gccgtcgagc aacagtatcc agtgacgcgc cgcacactca
    22501 ctattaatgg tgcagtggcg aaaccaataa cggtaaccgt ccccatcggc atgtctttac
    22561 gtgaagtcct ggcgctggcg ggcggcgcaa cagtagatgc tcctggtttt atcaatggcg
    22621 ggccgatgat gggaaatttg atcacatcgc tcgacacccc cgtcagcaaa accactggcg
    22681 gcttgctggt tttacccaac tctcattcac tgatccaacg tcgaatgcag aatgaccgca
    22741 gcgtacttac cgtcgcgaaa actgtttgtg agcaatgccg cttgtgtacc gatctctgcc
    22801 cacgtcattt agtcggtcac gaactggcac cccatttatt agtacgtgcg gttaactacc
    22861 aacagctcgc tacgccacaa ctgttactga cggcgctcac ctgttcggag tgcaacgtct
    22921 gcgccagcgt cgcctgtccg gtaggaattt caccaatgcg cataaaccgc ctgcttaagc
    22981 aggaattacg cgcacaaaat ctccgctacg aaggtgcctt aaatcctgct gatcccatgg
    23041 caaattatcg actgattccc gtaaaacggc tggtaaccag actgggccta atcccgtggt
    23101 atcaggatgc gccattgtct gaacaggtgc ctcagcccga aaaagtgacg ttattgttac
    23161 gccagcacat cggtgccagc gcaattgcgt gtgtacaaaa aggcgatcgc gtcgttcatg
    23221 ggcaatgtgt aggtcaaatt ccacacggca cattaggtgc ccccattcat gccagtattg
    23281 atggcatggt cagcgatgtg acagaaaacg cgattacgct tgtgagaggt taacgatgtc
    23341 gaaagcggta ggtattttag aacttagcag tatcgcgaaa gggatggaaa ccggcgacgc
    23401 catgctgaaa agcgcaaacg ttgagttact ggtcagcaaa acgctatgcc ccggaaaatt
    23461 tctgctgatg ctcggtggcg atgtgggtgc ggtgcaacag gccatcaaca gcggtgcttc
    23521 ccttgctggt gaaatgctgg tcgacagtct tgtcctgcca aatattcatc ccagtctcct
    23581 ttctgcaatc agcggactga accatgttga acagcaccgg gctgtcggcg tagtcgaaac
    23641 ctggagtgtc gccgcctgca ttgacgctgc cgattgtgct gtcaaagctg cgaatgtcat
    23701 tctcgttcgc gtacatatgg cttttggcat tggtggtaaa tgctacatgg tggttgctgg
    23761 cgatatttca gatgttaata acgcggtctc tgtcgccagc gagcgtgcgg gggaaaaagg
    23821 tttgttggtc tatcggtcag tgattgcccg tccacatgaa gccatgtggc gtcagatggt
    23881 ggagggataa tgacaactca gcacacaact gaacggatga ttcaggaata tgtccctggt
    23941 aagcaggtca cgctggcaca tgtcatcgcc aatcctggga aggatctgtt taagaaattg
    24001 ggtttaccag acaccgtttc tgctattggc attctgacga ttacccccag cgaagcatct
    24061 atcatcgcat gtgatatcgc cacaaaatca ggcgctgtag agatcggttt tcttgatcgt
    24121 tttaccggtg ccgtagtgct gactggtgat gtttctgcta ttgaatatgc acttagccag
    24181 gttacccgta cgctgggtga aatgatgcgc tttaccgcct gccccattac gcggacctga
    24241 cgttatgcaa cgtatgatgc ttatcggccc cagccagtgc ggaaaaacgt cactcacgca
    24301 atgtctgcgt ggcgaaaata tccattatca gaaaacgcag gcaatcgtct ggtccccgac
    24361 caccattgat acaccaggcg aatacctcga aaaccgcagc ctctacagtg cattgttggt
    24421 cagtgcctgc gaagcagaca ttatcgcgct ggtacttaat gcggacgcac cgtggtcacc
    24481 tttttcgcca ggctttacct cgtcaatgaa tcgcccggtc attggagtag taaccaaagc
    24541 agatttagcc agcatggaac aaatatcttt ggtgaaatgt tggttacggg aagctggagc
    24601 gcacaacgtg ttagtaacca gtgcggttaa taacaatgga gttacagaac tttttgcctt
    24661 gctgcataca gaagaaggct gccgctaatt tcgataggtt ctcagatgag aatgatgacg
    24721 gtgatacaca gataagaatc aatgagcaat gagggttgcc gggcaaccct cattgaataa
    24781 aacaggaatt acttcgcttc gccgtttttc atttcgccca tagctttcag ctttttggag
    24841 atatcgcggc gttctttgga gagctcggca tttttgatga tgtaatcgtc aacgcgatct
    24901 tcatagtcac ctttcatgct ggcgatgatg ccctggattg cttcaacgct catccctggc
    24961 ttgatgtaat cgctcaggtt gtccagcagc aggacgcgct tctggttgtc acggatcttt
    25021 ttctcaacgt cctgaatttc acgttgcagt ttgttcttac gacggaacag acgaacaaat
    25081 tccagtacgt cctggaatga aggcttggta gtttccattt ttatacccct gataatgtga
    25141 gagtcggatt cgtttaagca accgctattc gttagggcca acattactca tagctgccgc
    25201 agatgacaat gcttttatcc cttttcacta tcataccctt tatccttgct gaatcgaagc
    25261 agcagcaaga tgattctgaa gttcaggaat tatttgcgca aggcccggca aatcagattc
    25321 gatagcaact caagctgatg tgccgtttcc atgttcagcc acacataacc atagattggt
    25381 gtttccacaa ccttcaggtc atggtggtta tcgagcaact gacgcagctc ttccacagca
    25441 tcgttcaatt tttccgtatt ggcaaaaacc ggctgcggat taccttcgta cagcgcatga
    25501 acaaggctca gcagtatttg ctgcatcatg tgctgggtat cacgaagttt ttgcgcgttc
    25561 aataacacga aatggctggg gcgcgtggcc cagtatgcat tgatttgcaa ctccagcata
    25621 caaaccagat tgcgattaat ggtctggata ccttcatata tcgattttgg aatacgggtt
    25681 tctttgctgg cgggcgcaat cagcccacgc attttcacgg catcggtcag gagtttttgt
    25741 agatggcttt ccagacgtgg gcgttcgagt aagttcggtg agaatgcaga ttgatagacc
    25801 cgattatact cggtcagact tttcgccagt tgaatgcgcc agtggatgaa cgcccgttgt
    25861 ggccagatac cggtaaacaa cattgccagt aaagagccga ggatcacatc gccgcttcgc
    25921 cataacgccg tatcaatttc acctgtcggg gaacccacaa caattgccag cgtcacccca
    25981 atcaataaac cttgatatgg tttcttgccc agcgccagcc aaccgcaaag gaacatggcc
    26041 gccgcgcacc agactaacat cagcggtaac gagattaact ccagttgcag agcgataaga
    26101 cctaaaatcg accccaacac cgtaccgcca atacgttcga aggcacgagg gacaacgttc
    26161 ccccagaacg agattggccc cataatcacc accatggtga ccagcggcca ggtgctttcc
    26221 gggatagtaa acaggcggat aatgagaaaa gtgagcagga atgccagcgc gacccgagta
    26281 ccatgcacaa tgcggtaatg gcggtatacc cggatttcaa acgggcttaa tgacttatcg
    26341 gcacgcacgc agaaactccg taatgaagat taaaagcgct cataactcat ctgtcggcca
    26401 gaaggcgcgc ctctgaatta tccgacggac aaaccgggaa cagtgtaatt tcccggcagg
    26461 agcgaaaaaa ttgaaaggcg catcttactc ttttcgcttt caaaaaaaga gtggtcttcg
    26521 cgtcaacaca ccgccctgag atgaattagt gatgttttgg ctgcaccgca acatacattt
    26581 cgatatccca gtacccatct tccgcgccat tgttcaagta aacctcgaag cagggctttg
    26641 gtaacatttc ataagcactg tcctgcaaga ggctattaaa gaactgatac cagggtttag
    26701 caaaatcatc accgactaca cgagccaccg ccaccgcata ctgaccacct gaaatttctg
    26761 tcagaatgac gccctcactg ttttcgggga gcgtaaagtt attcggtacc gtcacgacgg
    26821 tgtcgcagcg taatttttcg gcgggtgttt catctggatt gtcgtaatag acagcaaccc
    26881 actccttcgg cacaatattt ttgctatcta tccacatcat caactgctca aaacctttct
    26941 ttaccgtctg ctcccacggg ccaacaagat ggaaacctgc aacggtacgt ttctcttcct
    27001 gcttaatctc gtagttcatg acgcctccat taacactgtt tttatataca gtatagttgc
    27061 aatattaaaa ccacaaggaa tgagtgttga ttatgcgagc agactcgcac tcctgccagt
    27121 ctgctgcaaa agaaaggtca ggccttatgg tggaaataat cactcaggcg ggaaaacatg
    27181 ccgccttccc cgacagattc cagggtaacc agcggccagt gcgccacctg tttatcacgg
    27241 tcgtaaagtt caatttcccc tacccgctga tgggcgctaa ttggcgcggt gagctcttta
    27301 ccatcaaggg tatatttggc tttgatatgt ggaatttcgg ctttcggtag caccatccag
    27361 aactcttgtt ccgttcccag gtcgatattt tctttatcgc cataccagat gcgttccgtt
    27421 ccgacctttt tcccacggtg caaaatttgc accgtagtaa agttttgttg cccccaacgc
    27481 agtaattttc ttgcctcttc ctcacgacct tttgcactgt cagcccccat taccactgca
    27541 atgagacgac gctgcccatc tacagccgaa gcaatgagat taaacccggc accagaagta
    27601 tgacccgttt tcaggccgtc aacattcatg gttttatccc acaacaaccc gttacggttt
    27661 tgctgggtga taccgttcca ggtgaggctt ttctcactgt acatatgata aaactcgggc
    27721 tcgccgtgga tgatagcgcg agaaagcaca gctaaatcat aagccgagct atgctggcca
    27781 ggtgcatcca gaccatgcac tgtttcaaaa tgcgtatcct tgagatgcag cttctcggca
    27841 tagttgttca tcattgcaac aaactgccgt tgcccaccgg caatatagtc agccagtgca
    27901 acacaagcat catttccgga atccacaatt aaaccacggc ttaaatcacg taccgatacg
    27961 cgatcgccct ctttcaaaaa catcagtgaa gaaccgacaa acactggatt atctttcgcc
    28021 cacgcatcgc gccctacggt gacaatatcg tctggcgtaa ttcgatgact atcgatagcg
    28081 cgatccacga cataacccgt catcagcttt gtcaggctgg cgggattgcg ctgttgatgc
    28141 tcattacccg cggtgaggat ctgtccggtg gtgtaatcca tcaataccca ggacccggca
    28201 tgaatctctg gaggctgagg tgaaaaagga atgttttccg ccgcaaaacc agacgataag
    28261 ttaaaaacga acaaagaagc agcaataata agacggcgtt tcaacagcaa accctcagga
    28321 gtttcaaata gctgttcttt ttacggaaat acttatgaac tggctggaat aaagtgcaag
    28381 aaaatgtgac taacctctca tttttatctg acatgatctg ttgccactcg ctgccaaatt
    28441 gtggcgctaa agctgattag cacggtgata tttgatactc tggcagacag cagagataac
    28501 ggatttaacc taatgatgaa tgacggtaag caacaatcta cctttttgtt tcacgattac
    28561 gaaacctttg gcacgcaccc cgcgttagat cgccctgcac agttcgcagc cattcgcacc
    28621 gatagcaaat tcaatgtcat cggcgaaccc gaagtctttt actgcaagcc cgcggatgac
    28681 tatttacccc agcctggagc agtattaatt accggtatta ccccgcagga ggcacgggcg
    28741 aaaggagaaa acgaagccgc gtttgccgcc cgtattcact cgctttttac cgtaccgaag
    28801 acctgtattc tgggctacaa caatgtgcgt ttcgacgacg aagtcacacg caacgttttt
    28861 tatcgtaatt tctacgatcc ttacgcctgg agctggcagc atgataactc gcgctgggat
    28921 ttactggatg ttatgcgtgc ctgttatgcc ctgcgcccgg aaggaataaa ctggcctgaa
    28981 aatgatgacg gtctaccgag ctttcgcctt gagcatttaa ccaaagcgaa tggtattgaa
    29041 catagcaacg cccacgatgc gatggctgat gtgtacgcca ctattgcgat ggcgaaactg
    29101 gtaaaaacgc gtcagccacg cctgtttgat tatctcttta cccatcgtaa taaacacaaa
    29161 ctgatggcgt tgattgatgt tccgcagatg aaacccttgg ttcacgtttc cggaatgttt
    29221 ggggcatggc gcggcaatac cagctgggtg gcaccgctgg cgtggcatcc tgaaaatcgc
    29281 aatgccgtaa ttatggtgga tttggcagga gacatttcgc cattactgga gctggatagc
    29341 gacacattgc gcgagcgttt atataccgca aaagccgatc ttggcgataa cgccgccgtt
    29401 ccggttaagc tggtgcatat caataaatgt ccggtactgg cccaggcgaa tacgctacgc
    29461 ccggaagatg ccgaccgact gggaattaat cgtcagcatt gcctcgataa cctaaaaatt
    29521 ctgcgtgaaa atccgcaagt gcgtgaaaaa gtggtggcga tattcgcgga agccgaaccg
    29581 tttacgcctt cagataacgt ggatgcacag ctttataatg gctttttcag tgacgccgat
    29641 cgtgcagcaa tgaaaattgt gctggaaacc gagccgcgta atttaccggc actggatatc
    29701 acttttgttg ataaacgaat tgaaaaactg ttgttcaatt atcgagcgcg caacttcccg
    29761 gggacgctgg attatgccga gcagcaacgc tggctggagc accgccgcca ggtcttcacg
    29821 ccagagtttt tgcagggcta cgctgatgaa ttgcagatgc tggcacaaca gtatgctgat
    29881 gacaaagaga aagtggcgct gttaaaagcg ctttggcagt acgcggaaga gatcgtct
//
LOCUS       DAZKGF010000043        29834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZKGF010000043 DAZKGF010000000
VERSION     DAZKGF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.043"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(55..798)
                     /gene="yjgL"
                     /locus_tag="ACHOQV_001845"
                     /pseudo
     CDS             complement(55..798)
                     /gene="yjgL"
                     /locus_tag="ACHOQV_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
     gene            complement(921..1373)
                     /gene="tabA"
                     /locus_tag="ACHOQV_001846"
     CDS             complement(921..1373)
                     /gene="tabA"
                     /locus_tag="ACHOQV_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFP9630230.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKANVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            complement(1518..2111)
                     /gene="bdcR"
                     /locus_tag="ACHOQV_001847"
     CDS             complement(1518..2111)
                     /gene="bdcR"
                     /locus_tag="ACHOQV_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9630231.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILEE"
     gene            2182..2895
                     /gene="bdcA"
                     /locus_tag="ACHOQV_001848"
     CDS             2182..2895
                     /gene="bdcA"
                     /locus_tag="ACHOQV_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9630232.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3027..3422
                     /gene="yjgH"
                     /locus_tag="ACHOQV_001849"
     CDS             3027..3422
                     /gene="yjgH"
                     /locus_tag="ACHOQV_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP9630233.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            3703..3837
                     /gene="pyrL"
                     /locus_tag="ACHOQV_001850"
     CDS             3703..3837
                     /gene="pyrL"
                     /locus_tag="ACHOQV_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFP9630234.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3841..4776
                     /gene="pyrB"
                     /locus_tag="ACHOQV_001851"
     CDS             3841..4776
                     /gene="pyrB"
                     /locus_tag="ACHOQV_001851"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFP9630235.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4789..5250
                     /gene="pyrI"
                     /locus_tag="ACHOQV_001852"
     CDS             4789..5250
                     /gene="pyrI"
                     /locus_tag="ACHOQV_001852"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFP9630236.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5323..5709
                     /gene="ridA"
                     /locus_tag="ACHOQV_001853"
     CDS             5323..5709
                     /gene="ridA"
                     /locus_tag="ACHOQV_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFP9630237.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5915..8611)
                     /gene="mgtA"
                     /locus_tag="ACHOQV_001854"
     CDS             complement(5915..8611)
                     /gene="mgtA"
                     /locus_tag="ACHOQV_001854"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP9630238.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGVYSRRYGWQ"
     gene            complement(8752..8805)
                     /gene="mgtL"
                     /locus_tag="ACHOQV_001855"
     CDS             complement(8752..8805)
                     /gene="mgtL"
                     /locus_tag="ACHOQV_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFP9630239.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8990..9937
                     /gene="treR"
                     /locus_tag="ACHOQV_001856"
     CDS             8990..9937
                     /gene="treR"
                     /locus_tag="ACHOQV_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFP9630240.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            10056..11477
                     /gene="treB"
                     /locus_tag="ACHOQV_001857"
     CDS             10056..11477
                     /gene="treB"
                     /locus_tag="ACHOQV_001857"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFP9630241.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11527..13182
                     /gene="treC"
                     /locus_tag="ACHOQV_001858"
     CDS             11527..13182
                     /gene="treC"
                     /locus_tag="ACHOQV_001858"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP9630242.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKASPYRQFYIWRDGESETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRRLMTVGEMSSTSLEHCQRYATLTGSELSMTFN
                     FHHLKVDYPSGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            13567..15705
                     /gene="nrdD"
                     /locus_tag="ACHOQV_001859"
     CDS             13567..15705
                     /gene="nrdD"
                     /locus_tag="ACHOQV_001859"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP9630243.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRSQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15863..16327
                     /gene="nrdG"
                     /locus_tag="ACHOQV_001860"
     CDS             15863..16327
                     /gene="nrdG"
                     /locus_tag="ACHOQV_001860"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFP9630244.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16372..16758)
                     /gene="cybC"
                     /locus_tag="ACHOQV_001861"
     CDS             complement(16372..16758)
                     /gene="cybC"
                     /locus_tag="ACHOQV_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP9630245.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16941..18293)
                     /gene="pmbA"
                     /locus_tag="ACHOQV_001862"
     CDS             complement(16941..18293)
                     /gene="pmbA"
                     /locus_tag="ACHOQV_001862"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFP9630246.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18387..18938
                     /gene="yjgA"
                     /locus_tag="ACHOQV_001863"
     CDS             18387..18938
                     /gene="yjgA"
                     /locus_tag="ACHOQV_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFP9630247.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIEQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(19096..20469)
                     /gene="mpl"
                     /locus_tag="ACHOQV_001864"
     CDS             complement(19096..20469)
                     /gene="mpl"
                     /locus_tag="ACHOQV_001864"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFP9630248.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20645..21643
                     /gene="fbp"
                     /locus_tag="ACHOQV_001865"
     CDS             20645..21643
                     /gene="fbp"
                     /locus_tag="ACHOQV_001865"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFP9630249.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21676..22671)
                     /gene="yjfF"
                     /locus_tag="ACHOQV_001866"
     CDS             complement(21676..22671)
                     /gene="yjfF"
                     /locus_tag="ACHOQV_001866"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFP9630250.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22658..23680)
                     /gene="ytfT"
                     /locus_tag="ACHOQV_001867"
     CDS             complement(22658..23680)
                     /gene="ytfT"
                     /locus_tag="ACHOQV_001867"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFP9630251.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            complement(23694..25196)
                     /gene="ytfR"
                     /locus_tag="ACHOQV_001868"
     CDS             complement(23694..25196)
                     /gene="ytfR"
                     /locus_tag="ACHOQV_001868"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFP9630252.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25336..26292)
                     /gene="ytfQ"
                     /locus_tag="ACHOQV_001869"
     CDS             complement(25336..26292)
                     /gene="ytfQ"
                     /locus_tag="ACHOQV_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFP9630253.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26602..27132
                     /gene="ppa"
                     /locus_tag="ACHOQV_001870"
     CDS             26602..27132
                     /gene="ppa"
                     /locus_tag="ACHOQV_001870"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFP9630254.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27590..28585)
                     /locus_tag="ACHOQV_001871"
     CDS             complement(27590..28585)
                     /locus_tag="ACHOQV_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9630255.1"
                     /translation="MSIKKLADGKYCVDVRPAGSEGRRFRRRFPTRGEAAMYERHVLT
                     HYHDKDWVEKPIERKALSDLLELWWVYHGKNHRYGAMNRVRIEAVLRDMQELGINRSD
                     HLTRKNIIRYRLELMNRGIKQSTVNRYCAMMSGFFEKLIDVEEFVGDNPFHEIRKLTI
                     NQPEMAYLAHDDIPRLTALLSGDNLKAVLLSLATGGRWGEVANIKGEHIIGGKVIFME
                     TKNGSRRVIPIAKDLESLIKVKATGRLMNPSYAIVRSAIRTVRPDLPVGQSVHVLRHT
                     FATHFMINGGNIITLQRILGHSTIQQTMTYAHFAPDYLQDAVRFNPVAELSRFCP"
     gene            complement(28652..28951)
                     /locus_tag="ACHOQV_001872"
     CDS             complement(28652..28951)
                     /locus_tag="ACHOQV_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9630256.1"
                     /translation="MSTNVGEKIRIMRKSEMLTQEKMAEITGLTVAALRQYEQGRNEP
                     NLESTKKLLKSPVFRKYRDWFLFDEVEPKAGQVVPALAHIGRSESESPHSGKKTG"
     gene            29059..29484
                     /locus_tag="ACHOQV_001873"
     CDS             29059..29484
                     /locus_tag="ACHOQV_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFP9630257.1"
                     /translation="MQEVTQNAPESPAETQCDQLTDAPETAHQKTGRKAPERQEIRLS
                     QNPSHLLSKEGFAMYIGKTVDAIVSMAKAGKLPAVYMADPLKPGGNAELYINRKAWDE
                     ACDRLTENAPQEWHEWENRLFLFKPAAGSRNKRISKKAA"
BASE COUNT         7481 a         7872 c         7334 g         7147 t
ORIGIN      
        1 cattaatagc agatgttcgt tccttattgc taataaggct ttattcaatg cttattctaa
       61 taaatccgta atcgtagaat caactgaacc tttacaactc acctgcgagt tatttaagtc
      121 ctgcgagtta tttaactcct gagaatcatt taaatcctgc gaattattta actcctgcga
      181 attatttaac tcctgtatat tatttaactt ctgtaaatta tttaacttct gatgattatt
      241 taatagacgc gctatttcct tgttagcttc gcgttggtaa cacgccagaa ccttgttagt
      301 aatcaggtgt aatgaactat catcttttct ttcaaaagac ttcccttcat aacgattagt
      361 cgaaccatca gattgttgaa atataatagc cgtatgtcct tgacgagtcc ccatagtagt
      421 gatagtggaa agtgtaattt ctatttccac cttagataaa tccgctcgtt ccccttcagg
      481 attattcatg taaaacttca ttttttcatt gccatcagta tctttagaat aagatgagga
      541 ctcaatatga gccatcatat ttctacccgc ttcgttggag cgtttgctaa aatccacata
      601 acccagggta aatatctttg ccaaaaactc taaaaatgct ccaggagaaa gaatattcct
      661 tctgctatcc tgccccccgg caatacgggc aaaatcattc attccaacac gccaggtatt
      721 taaaacttca cttttttgtt taaaattgtc ggctaatgta atgtgttgag aataattcaa
      781 atctgatatt ttgctcatga cgaactcctt gagttaataa gaacgtctaa gcattacaaa
      841 ttttactcac aaaaaaatat caaaaaacgc tcttttcaca aactcgccgt aaacctggag
      901 ttatttacgg cgaaaagtca tcacgccatc agcatcttca ctactgcttt gcgaacccgg
      961 gctggcgcgc ccactgcgca cagcggttta tgcacttccc ccggataaaa cacaacaaaa
     1021 tcaccttcat taaggataac tgttttctca tcaacgcctt ccggcaaaaa tgcgatgtct
     1081 ttatcagcta accagtcggt atccggcgta cctgcaggtt gtgtgctgaa ggtcatgcct
     1141 tcctgacctt ttaacacaat ctgaatgtcg agatagcggg catggtactc cgcacggcga
     1201 gcttcgtacg gctcggtcat atcttccgag ataagataaa acagacgatt gccttcgata
     1261 tcgtgcttgc ccttgggcgt ttctgccgta acgtttgctt tgatatgctc aatcgcctgg
     1321 cgtaactctt ggggtagcca cggctgaaga ttatgaatat ttccgatgat catcttttcc
     1381 actccttaat taaaacaacg tttcatttct atcgttatac gaaaagatga tcaattccgc
     1441 tagccttaac gcgtgaatta ttgtcttatc acaacttatc ggcaggcgcg ttcccgcctt
     1501 tcgggtcaag gcccgcatca ctcctcgaga atagttttta tcgcttcccc cgccagggca
     1561 gctgttgcac agagttgttc gatggagtgc ccctcccgtg ccttcgcaga aagccctgac
     1621 atcacggtac tcataaaatc agtcacacat tgtgcgcttt gtggatgccg cctggcgata
     1681 tagtcataaa tggtcgtttc tgcggcgtga taatattgaa tggcaatatc acgcgcttgt
     1741 ggatcatgac tatgaatacc ttcaagaacc atacagccag cgcagccgcc gttttggcta
     1801 tatctgcgcg ccgcttcttt taatacctca accaggcact cgcctaccgg acgatcatca
     1861 cgaagaatat cggcaagcgg aatagcttcc gtaccgacat attcattgag tacacggcta
     1921 aacaacccag ccttactgcc aaaagccgcg tagaggctcg gggggttaat accaagataa
     1981 tcagtaactt cagatacact tactgcatcg aaaccttttt ggtgaaaaag cacttttgcc
     2041 gcagagactg cctgctcagg agcgaaacgt ctggggcgac ctggaacgcg agattgtttt
     2101 ttagtgacca ttacaaaact tgttgacaga aagttaaaac agttttgtaa tgcatgttac
     2161 ataataaatc aaggagtcct tatgggcgct tttaccggta agacagttct catcctcggt
     2221 ggtagccgtg gaatcggtgc cgctatcgta cgtcgtttcg tcaccgatgg agccaatgta
     2281 cgattcacct atgcggggtc gaaagatgcc gctgaacgtc tggcacaaga gactggagcg
     2341 acagcagtat tcacagatag tgctgacaga gacgctgtta ttgatgtcgt tcgtaagagc
     2401 ggcgcattgg atatcctggt ggtaaatgca ggtattggcg tctttggcga tgccctggaa
     2461 ttaaatgccg acgatattga tcgccttttc aaaatcaata ttcatgctcc ttatcatgcc
     2521 tctgttgaag ccgcccggca gatgcccgaa ggcgggcgca tcttaatcat cggctccgtg
     2581 aatggcgatc gtatgcctgt tgcaggcatg gctgcttatg ccgccagtaa atctgccctg
     2641 caaggcatgg cgcgcgggct ggcccgtgat tttggaccgc gagggatcac catcaacgtc
     2701 gtccagccag gaccaattga taccgacgct aatcccgcca acgggccaat gcgcgatatg
     2761 ttgcatagtt ttatggccat caaaagacat gggcaaccgg aagaggtcgc tggtatggtc
     2821 gcatggttag cagggccaga agccagtttt gttaccggcg cgatgcatac cattgatggc
     2881 gcgtttggcg cataaccgac tacgctcaat taagcccagc cattatccat gatgtctggg
     2941 ttttgtttac taacgtcgtc cccctaaaag cggctcccgg taaatataaa tcttctgttc
     3001 atgtcgtaaa tcttgaggat aaccaaatgg tagaaagaac cgctgttttc cctgctggcc
     3061 gacattcact gtatgctgag catcgttatt ctgcggctat tcgttccggc gatttgctgt
     3121 ttgtttccgg gcaagtagga agtcgagagg acggaacacc agaacccgat tttcagcaac
     3181 aagtcagact ggcatttgat aatttgcatg cgaccctggc tgctgcggga tgcacttttg
     3241 acgatatcat tgatgttacg agctttcata ccgatccaga aaaacaattt gaagacatca
     3301 tgacggtcaa aaatgaaata tttagcgccc caccttatcc aaactggacg gcggtgggtg
     3361 ttacctggct ggcaggcttt gattttgaaa ttaaagtgat agcgcgcatc cctgagctat
     3421 aagcaatagt gttagccgtt cgttttcaca ctccgcccta taagtcggat gaatggaata
     3481 aaatgcatat ctgattgcgt gaaagtgaaa aagaaaaaag caggaaatgt ctgcaattat
     3541 tgataccgaa cgacagttct cccccagaat cacatcaaat aaaaatgcat gtattttgac
     3601 ttttaattca aataaaccgt ttgcgctgac aaaatattgc atcaaatgct tgcaccgctt
     3661 ctgacgatga gtataatgcc ggacaatttg ccgggaggat gtatggttca gtgtgttcga
     3721 cattttgtct taccgcgtct gaaaaaagac gctggcctgc cgtttttctt cccgttgatc
     3781 acccattccc agcccctcaa tcgaggggct tttttttgcc caggcgtcag gagataaaag
     3841 atggctaatc cgctatatca gaaacatatc atttccataa acgaccttag tcgcgatgac
     3901 cttaatctgg tgctggcgac agcggcgaaa ctgaaagcaa acccgcaacc agagctgttg
     3961 aagcacaaag tcattgccag ctgtttcttc gaagcctcta cccgtacccg cctctctttc
     4021 gaaacttcca tgcaccgcct gggtgccagc gtggtgggct tctccgacag cgccaataca
     4081 tcactgggta aaaaaggcga aacgctggcc gataccattt cggttatcag cacttacgtc
     4141 gatgcgatag tgatgcgtca tccgcaggaa ggtgcggcgc gcctggccac cgaattttct
     4201 ggcaatgtac cggtactgaa tgccggtgat ggctccaacc aacatccgac gcaaaccttg
     4261 ctggacttat tcaccattca ggaaacccag gggcgtctgg acaatctcca cgtcgcaatg
     4321 gttggtgacc tgaaatatgg ccgcaccgtt cactccctga cccaggcgct agcgaagttc
     4381 gacggcaacc gtttttactt tatcgcgccg gatgcgctgg caatgccgca atacatcctt
     4441 gatatgctcg atgaaaaagg gatcgcctgg agtctgcaca gctctattga agaagtgatg
     4501 gcggaagtag acatcctgta catgacccgc gtgcaaaaag agcgtctgga cccgtccgag
     4561 tacgccaacg tgaaagcgca gtttgttctt cgcgccagcg atctccacaa cgccaaagcc
     4621 aatatgaaag tgctgcatcc gctgccgcgt gttgatgaga ttgcgacgga tgtggataaa
     4681 acgcctcacg cctggtactt ccagcaagca ggcaacggga ttttcgctcg ccaggcgtta
     4741 ctggcactgg ttctgaatcg cgatctggta ctgtaagggg aaatagagat gacacacgat
     4801 aataaattgc aggttgaagc tattaaacgc ggcacggtaa ttgaccatat ccccgcccag
     4861 atcggtttta agctgttgag tctgttcaag ctgaccgaaa cggatcagcg gatcaccatt
     4921 ggtctgaacc tgccttctgg cgagatgggc cgcaaagatc tgatcaaaat cgaaaatacc
     4981 tttttgagtg aagatcaggt agatcaactg gcattgtatg caccgcaagc cacggttaac
     5041 cgtatcgaca actatgaagt ggttggcaaa tcgcgcccca gtctgccgga gcgcatcgac
     5101 aatgtgctgg tctgcccgaa cagcaactgt atcagccatg ccgaaccagt ttcttccagc
     5161 tttgccgtgc gaaaacgcgc caatgacatc gcgctcaaat gcaaatactg tgaaaaagag
     5221 ttttcccata atgtggtgct ggccaattaa ttgcggttgg taataaaagt ctggctccct
     5281 ataatgagcc agacttttta ccgctgtaat aaaggagaaa tcatgagcaa aactatcgcg
     5341 acggaaaatg caccggcagc tatcggtcct tacgtacagg gcgttgatct gggcaatatg
     5401 atcatcacct ccggtcagat cccggtaaat ccgaaaacgg gcgaagtacc ggcagacgtc
     5461 gctgcacagg cacgtcagtc gctggataac gtaaaagcga tcgtcgaagc cgctggtctg
     5521 aaagtgggcg acatcgttaa aaccaccgtg ttcgtgaaag atctgaacga cttcgcaacc
     5581 gtaaacgcca cttacgaagc gttcttcacc gagcacaatg ccaccttccc ggcacgttct
     5641 tgcgttgaag ttgcccgtct gccgaaagac gtgaagattg agatcgaagc aatcgctgtt
     5701 cgtcgctaat cttgatggaa atccgggcta tcatgcccgg attaagtctg atgacaaaag
     5761 caaaatcgcc cgatgcgcta cgcttatcag gcctacgtga ttcctgcaat ttattgaatt
     5821 tgtaggccgg attgggcgtt tacgccgcat ccggcatgaa caaagctcac tttgtctgca
     5881 atctgaatcc gggccaccat gcccagttta ttctttactg ccagccgtaa cgacggctat
     5941 agaccccttt caccaactgg gttaacgtca tataccctgc cagaatcgca accagccacg
     6001 ggaaatagct taacggcagc gcctgcagct gcagataact ggccagcggt gaaaacggca
     6061 atgcgatccc gacaatcatc acgatcacgg tcatgatcat taacggccac gatgcacagc
     6121 tctgaataaa cggtacacga cgggtacgga tcatatgcac aatcagcgtt tgcgacagta
     6181 agcccaccac aaaccagccc gactggaaca gcgtttgcgt ttccggcgtg ttggcatgga
     6241 atacccacca catcaggcaa aacgtcagaa tatcgaagat cgagctgatc ggtccgaaga
     6301 agatcataaa gcgccccaga tccgccggat tccaacgctg cggcttttga atttgctcgt
     6361 cgtcgacgtt atcaaacggg atcgccacct gtgacacatc gtacagcaag ttctgaataa
     6421 gcaagtgtaa cggcaacatc ggcaggaagg gcaagaaagc actcgccacc agcacgctga
     6481 acacattacc gaagttagaa ctcgccgtca ttttgatgta tttcagcatg ttggcgaaag
     6541 tgcgacgtcc ctcaataacc ccctcttcca gcaccatcag gcttttttcc agcaggatga
     6601 tatcagccgc ttcacgggca atatctaccg cgccgtccac agaaatgccg atatccgcag
     6661 cgcgtaaggc gggcgcatca ttaataccat cgcccataaa gccaaccaca tgcccttcgc
     6721 gcttcagtaa ggtcacaatg cgttctttat gcatcggcgt caggcgggca aacagcgtgg
     6781 tgcgctgtgc gagatttgcc agttcatcgt cagatagcgt ttcaatatca ctaccaatga
     6841 ccacctctcc cgcatccagc cccacttcat ggcacacttt cgctgcaact aactcactgt
     6901 cgccagtaag gatttttacg gtaattccac tcgcttttaa tgccttcagt gccggagcgg
     6961 ttgtctcttt tggcggatca aggaaagcaa tatatccttc gaggatcagg tcggattcat
     7021 ccgcccgctg gtaatctcct tcacgcgctg gcaggtattt cgtcgccacc gcaaccacgc
     7081 gcagcccctg acgattcagc gtatcagtaa cccgcttaat cttacgcagc atgatgtcat
     7141 cgagcggcac aatctcgcca ttgtgacgca cctgcgaaca cacattgagg atttcctgca
     7201 atgcaccttt gcaaaccagc tgatggtgtt cggtattttc tgccactacc accgacatcc
     7261 gacgacgctc gaaatcaaac ggaatctcat caattttttg ccaacggctg gccagcgaac
     7321 gcgctgactc ttcatccgta ccttcgagca ccgcggtatc aagcaggttt ttaagtccgg
     7381 tctggtaatg actgttcaac cacgcgctat gcagcacgcg ttcgctggtt ttaccggaga
     7441 tatcggtatg attctccagc acaattttgt cctgcgtcag ggtgccggtt ttatcagtgc
     7501 acagaatatc cattgcgcca aagttctgaa tggcatccag atgtttgacg atcactttct
     7561 gtttcgacag ctttactgcg ccgcgcgcca gcgtcgaggt aacaatcatc ggcaacattt
     7621 ctggcgttaa gcctaccgcg accgaaagcg caaacagtgc cgcttcccac cagtcgcctt
     7681 tggtgtaacc gttgataagc aacaccaccg gagccatcac cagcataaag cgaatcagca
     7741 gcatactgac gcggctaatc ccttgctgaa aggcattcgg ctcgctttcc tgctcactaa
     7801 cacgccccgc cagttgacca aaccaggtat tggcccctgt ggcaatcacc attgcttgtg
     7861 ccgtaccgct caccacggtg gtgcccataa aacacagggt gtcgcactcc agcggattgc
     7921 tgtgctccgg ctggcgagta gtagcggctt tttccacggg cagagactca ccggttaacg
     7981 acgcctgagc gacgaacaga tcccgcgcct gcaagatacg taaatctgcc gggatcatat
     8041 cccctgccgc cagttttata atatcgccgg gcaccagttg gtcgatcggg atctccagcc
     8101 agccattttc gcctttgtcg ttaattacgc gcagcaccgt cgcagtattg ctgaccatcg
     8161 ctttcagggc atctgccgct ttagtggaac gtgcttcctg aataaagttc agcaacgtag
     8221 aaatagcgac cattagcgcg ataacgcccg cggcaaataa atcttccgtg gcgtaagaaa
     8281 tagcgccgag aatggtgagt aaaatattaa aggggttgcg atagcagacc cataaatgaa
     8341 cccaccacgg cgacggttgt tgtgcgggta atttattttc accatgttgt tcgcgggcag
     8401 attccacttc cgcctgattt aatccttccg gatgggtgtc gaacgttttc cacaattctt
     8461 cttcgggcat caccgccatt ttcaggcaat gcgcacttaa ggacggcggg accgccgtat
     8521 tcactgtctg ctgcgcgcca ggcaatggat cgcgatgaac cagacgggaa ggtaaatggc
     8581 gaatgaaccg ggtaaaaatt tctttaaaca taaggagtcc ctccgcactg tctgaaacag
     8641 tacagaaatg tcttcaggcg tggcgaagcc ttacctgaag gtaataaagc tgagcaggga
     8701 cgttaaaacg tcacggcctt acgcatccgg taacgccgtg aaaagacggg cttaccggaa
     8761 aagttttaat ctccgtcgag ggagaggcgt gggatcaggt tccatataac ctccggtaag
     8821 tgaaaaaagc gccgcaaata ataaggagaa tccaataata ttcgtagcat ttatggcgat
     8881 ataatacctg ctggcaacta aaccaacgat aaaccagact ttacccattg ctgaatgcac
     8941 aggtaaagtt aggctcaaat aattaaacaa cacgttatgg gacaacagga tgcaaaatcg
     9001 gctgaccatc aaagacatcg cgcgcttaag cggcgtgggg aaatctacgg tttcccgggt
     9061 gctgaataac gaaagcggcg tgagccagcg cacgcgcgag cgtgttgaag cagtgatgaa
     9121 tcagcatgga ttttcccctt cccgctctgc gcgcgctatg cgtgggcaaa gcgataaagt
     9181 ggtcgccatc attgttaccc gtctggattc gttgtcagaa aatctcgccg ttcaaaccat
     9241 gctgccagcg ttctatgaac aaggttacga tccaatcatg atggaaagtc agttttcccc
     9301 acaattagtt gccgaacatt tgggggtgct gaaacggcgt aatatcgacg gcgtagtgct
     9361 gttcggtttt accggcataa cagaagaaat gttagcccac tggcagtcat cgctggttct
     9421 gctggcgcgt gacgcaaaag gctttgcttc ggtctgttat gacgacgaag gggcaatcaa
     9481 gatcctgatg caacggctgt atgaccaggg acatcgtaat atcagttatc tcggcgtgcc
     9541 gcacagtgac gtgacgaccg gtaagcgacg tcacgaagcc tacctggcgt tctgcaaagc
     9601 gcataaattg catcccgttg ccgctctgcc agggcttgct atgaagcaag gctatgagaa
     9661 cgtagcaaaa gtgattacgc ctgaaactac cgcgttactg tgcgcaaccg acacgctggc
     9721 acttggcgca agtaaatacc tgcaagagca acgcatcgac accttgcaac tggcgagcgt
     9781 cggtaatacg ccgttaatga aattcctcca tccggagatc gtcaccgtag atcccggtta
     9841 cgccgaagct ggacgccagg cggcctgcca gttgatcgcg caggtaaccg ggcgcagcga
     9901 accgcaacaa atcatcattc ccgccaccct gtcctgatcg tttcctgaac gataaattgt
     9961 gatcttcgct gcgtttcggg aacgttcccg tttttaaatt tttcagcgca atatattctg
    10021 cagccaacca aaaatgtcat ctgccatggg gctttatgat gagcaaaata aaccaaacgg
    10081 atatcgatcg gttgattgaa ctggtcggcg ggcgcggcaa tattgcgacg gtgagccact
    10141 gtattactcg tctgcgcttt gtcctcaacc aaccggccaa tgccagaccg aaagaaattg
    10201 agcaactccc catggtgaaa ggctgtttca ccaatgccgg gcaatttcag gtggtgattg
    10261 gcaccaacgt gggtgattac tatcaagcac tgatagcgtc aaccggacag gcgcaggttg
    10321 ataaagagca ggtaaaaaaa gccgcccggc agaatatgaa atggcatgag cagttgatct
    10381 ctcatttcgc ggagattttc ttcccgttgc tgcccgcgtt gattagcggc ggtttgatcc
    10441 tcggttttcg caatgtgatc ggcgatttgc ccatgagcaa cggtcaaacg ctggcgcaaa
    10501 tgtacccttc cctgcaaacg atctacgatt ttctgtggtt gatcggtgaa gcgatcttct
    10561 tctacctgcc ggtcgggatt tgctggtcag cggtgaaaaa aatgggcggc acgccgatcc
    10621 ttggtatcgt gcttggcgta acgctggttt ctccacagct gatgaacgct tatctgctcg
    10681 ggcagcagct gccggaagtg tgggactttg gcatgttcag catcgccaaa gtgggctatc
    10741 aggcgcaggt gatcccggca ctgttagccg ggctggcgct gggcgttatt gaaactcgtc
    10801 ttaagcgcat cgtgccggat tacctctatc tggtggtggt gcccgtttgt tcgctgatcc
    10861 tcgcggtgtt cctcgcccat gcgctgattg gtccgtttgg tcgcatgatt ggcgatggcg
    10921 ttgcctttgc ggtacgtcac ctgatgaccg gcagctttgc tccgattggc gcggcattgt
    10981 ttggcttcct gtacgccccg ctggtgatca ccggcgtaca tcagaccacg cttgctattg
    11041 atttgcagat gattcaaagc atgggtggta cgccagtgtg gccgctgatt gcgctgtcga
    11101 atatcgctca gggttccgcc gtgataggca ttatcatttc cagccgcaag cacaatgaac
    11161 gcgagatctc cgtgcctgcc gctatctccg cctggcttgg agtcactgag cctgctatgt
    11221 acggcatcaa cctgaaatat cgcttcccga tgctgtgcgc gatgattggt tctggtctgg
    11281 caggattgct atgcggcctg aacggcgtta tggcgaatgg catcggcgta ggcggcctgc
    11341 cgggaattct ctcgattcaa ccgagctact ggcaggtgtt tgcgctggca atggctatcg
    11401 ccatcatcat cccgattgta ctcacctcgt ttatctatca gcggaaatac cgcctgggca
    11461 cgctggacat tgtttaattt tcttcggggc gcaattgcgc cccctcgcat ttgcaggaat
    11521 aacgtaatga ctaatcttcc ccactggtgg caaaacggcg ttatctacca gatttatcca
    11581 aagagttttc aggacaccac gggtagcggc accggcgatt tacgtggcgt tatccagcgc
    11641 ctggactatc tgcataaact gggcgttgat gccatctggc taaccccctt ttatgtctct
    11701 ccccaggttg ataacggtta cgacgtagcg aactatacgg ctattgatcc cacctacggc
    11761 acgctggacg attttgacga actggtgaca caggcaaaat cgcgcgggat tcgtatcatt
    11821 ctcgatatgg tgtttaacca tacctctacc caacatgcct ggtttcgcga ggcgctgaac
    11881 aaagcaagcc cttaccgcca gttttatatc tggcgcgatg gagaatcaga aacgccaccg
    11941 aacaactggc gttcaaaatt tggcggtagt gcgtggcgct ggcatgcgga aagcgaacag
    12001 tactatttgc atctctttgc accagaacag gcggatctca actgggagaa tccagcggta
    12061 cgcgcagagc tgaaaaaagt ctgtgagttc tgggccgatc gtggggtcga cgggttgcgc
    12121 ctggatgtgg tgaatctgat ctccaaagac ccgcgtttcc ctgatgacct ggatggcgac
    12181 gggcgtcgct tctacaccga cgggccacga gcacacgagt ttttgcacga gatgaaccgc
    12241 gatgtgttta cgccacgcag gttaatgacc gtaggtgaaa tgtcctccac cagccttgag
    12301 cattgccagc gatatgcgac actgacaggc agtgaattgt cgatgacctt taattttcat
    12361 cacctgaaag tcgattatcc cagtggtgaa aaatggacgc tggctaaacc tgactttgtg
    12421 gcgttgaaaa cattgttccg ccactggcaa caaggaatgc acaacgtggc atggaatgcc
    12481 ttgttctggt gtaaccacga tcagccgcgc attgtttctc gctttggtga tgaaggtgaa
    12541 taccgcgtgc ctgcggcaaa aatgctggcg atggtgctgc atggcatgca gggaacgccg
    12601 tatatctacc agggcgaaga gattggcatg accaacccgc atttcacgcg cattactgac
    12661 tatcgcgacg tggagagcct caatatgttt gccgagctgc gcaacgatgg tcgtgatgcc
    12721 gacgagttat tggcaatcct cgccagtaaa tcccgtgaca acagtcgcac acccatgcaa
    12781 tggagcaacg gcgataatgc cgggtttacg gctggcgaac cgtggattgg cctgggcgat
    12841 aactatcagg aaatcaacgt agaagcagcg ctggccgatg agtcctcggt gttttacacc
    12901 taccaaaagt taatcgcact gcgtaagcaa gaagccgtcc tgacatgggg cgattaccag
    12961 gatctgctgc caaacagccc tgtattgtgg tgctatcgcc gtgagtggaa ggggcaaacc
    13021 ttactggtca ttgccaacct tagccgtgag acccaaccct ggcagccagg gaaaatgctc
    13081 ggcaactggc agcttgtgat gcataactac gaagaagcct caccacaacc ctgtgccatg
    13141 actttacggc cttttgaggc tgtctggtgg ttacagaagt aagtatccgc taagccccgg
    13201 taatactggt cattccgggg ttttgccttt cagcatgttg attttataat caaaaaattc
    13261 attaaaaaat ttacataacg ctgtagcgcc cgtcatccgt acgctctgct ttttactttg
    13321 agctacatca aaaaaagttc aaacatcctt gatgcaaagc actatatata gactttaaaa
    13381 tgcgtcccga cccaatatgt tgtattaatc gactataatt gctactacaa ctccccacga
    13441 aaaaggtgcg gcgttgtgga taagcggatg gcgattgcgg aaagcgccgg aaaacgaaac
    13501 gaaaaaaccg gaaaacacct ttcccaattt ctgtggataa cctgttctta aaaatatgga
    13561 gcgatcatga caccgcatgt gatgaaacga gacggctgca aagtgccgtt taaatcagag
    13621 cgcatcaaag aagcgattct gcgtgcagct aaagcagcgg aagtcgatga tgcagattat
    13681 tgcgccactg ttgccgcggt tgtcagcgag cagatgcagg gccgcagcca ggtggatatc
    13741 aacgagatcc agaccgccgt tgaaaaccag cttatgtcgg gtccatacaa acaactggct
    13801 cgtgcttaca tcgagtaccg tcacgatcgc gacattgaac gtgaaaaacg cggtcgcctg
    13861 aatcaggaga tccgtggtct ggttgagcag accaacgcct cgttgctcaa cgaaaacgcc
    13921 aacaaagaca gcaaggtgat tccaacccag cgcgacctgc tggccgggat tgtggctaaa
    13981 cactatgcac gccagcacct gctgccgcgt gacgtggtgc aggcacatga acgtggtgat
    14041 attcactatc acgatctcga ttactcaccg ttcttcccga tgttcaactg catgttgatc
    14101 gacctgaaag gcatgctgac ccagggcttt aaaatgggga acgccgagat tgaaccgccg
    14161 aagtcgatct ctacggcaac cgcggtaact gcgcagatta ttgcccaggt cgccagccat
    14221 atttatggcg gcaccaccat taaccgtatc gatgaagtgc tggcaccgtt tgtcaccgcc
    14281 agctacaaca aacatcgcaa gaccgcagaa gagtggaaca tcccggacgc cgaaggctac
    14341 gctaactctc gtaccatcaa agagtgctac gatgccttcc agtcgctgga gtacgaagta
    14401 aacaccctgc acaccgccaa cggtcaaacg ccgtttgtaa cctttggttt tggtctgggc
    14461 accagctggg agtcgcgcct gattcaggaa tctatcctgc gtaaccgtat cgccggcctc
    14521 ggtaaaaacc gtaaaacggc ggtgttcccg aaactggtgt ttgcgattcg cgatggactg
    14581 aaccataaaa aaggcgatcc gaactacgac atcaaacagc tggcgctgga gtgcgcaagc
    14641 aagcgcatgt atccggatat cctgaactac gatcaggtag tgaaagtcac cggttcgttt
    14701 aaaaccccga tgggctgccg cagcttcctc ggcgtgtggg aaaatgaaaa cggcgagcag
    14761 atccacgatg gtcgtaacaa cctcggcgtg atcagcctga acctgccgcg tattgctctg
    14821 gaggcaaaag gcgatgaagc caccttctgg aagctgctgg atgaacgtct ggtgctggca
    14881 cgtaaggcgt tgatgacccg tatcgctcgt ctcgaaggcg tgaaagcgcg cgtggccccg
    14941 atcctctata tggaaggtgc ttgtggcgtg cgtctgaatg ctgacgacga cgtttctgaa
    15001 atcttcaaaa acggtcgtgc gtctatttcg ctgggttaca tcggcatcca cgaaaccatt
    15061 aacgcgctgt tcggcggcga gcatgtttac gacaacgagc agctgcgcgc gaaaggtatt
    15121 gcgattgttg agcgtctgcg tcaggcggtg gatcagtgga aagaagaaac gggttatggt
    15181 ttcagtctct acagcacgcc aagtgaaaac ctgtgcgacc gcttctgccg tctcgatacc
    15241 gctgagtttg gcgtggtgcc gggcgtgacc gataaaggtt actacaccaa cagtttccac
    15301 cttgatgtgg agaaaaaggt gaacccgtat gacaagatcg actttgaagc gccttacccg
    15361 ccgctggcga acggtggttt catttgctac ggcgagtatc caaacattca gcacaacctg
    15421 aaggcgctgg aagatgtctg ggattacagc tatcagcatg taccgtatta cggcaccaat
    15481 acgccgatcg atgagtgcta cgagtgtggc tttaccggtg agttcgagtg caccagcaaa
    15541 ggcttcactt gtccgaaatg tggtaaccat gacgcctccc gtgtgtcggt gactcgccgc
    15601 gtgtgcggat atttaggtag cccggatgca cgtccattta acgccggtaa gcaggaagaa
    15661 gttaagcgcc gcgttaaaca tttggggaat gggcagatag gttaatcttc actctgcgtt
    15721 aagcaaatgc cggatgcggc gtaaacgcct tatccggcct acatttggca acggctgtag
    15781 gcctgataag acgcgacagc gtcgcatcag gcgttgatta ctggatgcag cctacacgcg
    15841 gactgataag ccaggcgaaa taatgaatta tcatcagtac tatcctgtcg acatcgtcaa
    15901 cggccccggc actcgctgca ccctgtttgt ctccgggtgt gttcatgaat gccccggttg
    15961 ctataacaaa agcacctggc gggtaaattc cggtcagcca tttaccaaag caatggaaga
    16021 ccagatcatt aacgatctga atgacactcg catcaaacgc cagggtattt ccctctccgg
    16081 cggcgatccg ctgcatccgc aaaacgtgcc ggatattctg aaactggtac aacgcatccg
    16141 cgccgaatgt ccgggtaaag acatctgggt gtggacaggc tataaactcg acgaactcaa
    16201 cgctgcgcaa atgcaggttg ttgatttgat taacgtgctg gtcgacggca aatttgtgca
    16261 ggatttaaaa gacccttccc tgatctggcg cggcagcagc aatcaggtgg tgcatcattt
    16321 gcgatgataa gttccctgtt acctgagtgc aggcaacagg gaaataagga attaacgata
    16381 cttctggtga taggcgttgc gggtcgtttt cagttgctct gcggcagcct gcgcttcttt
    16441 tactttacct tcatttgcca gcttcagcgc gtcgtcaatc tgaccgacca gaatatcgaa
    16501 accgtggcgg aagtctttca tttccgggct gtccggtgat ttgccttcga gcttcggcgg
    16561 cgtcgctttt tgcgcatcca gcgctgcggc gcgcatcttc gttaacgcgt ctttgacttg
    16621 cgccgcgtta tcggcttttt cgaccacttt taaattgtcg ttgagggttt ccatattgtc
    16681 ttcaagatca gcggcaaacg acgccgaact gaataccaac gaggagactg caagaatagc
    16741 taacaggctt ttacgcattg ctcacttcct tttttattta cacttcatcc ttcacactgc
    16801 ctcatcgacg gctacgctca ttcgcccgag tcacgtactg cgagtacgct ccccggtcct
    16861 cattcacttt ccgtctcgat gcagtgcgaa tgattttgtg taatttttta gtttatagcg
    16921 cggcaggtcg cgccagtttt ttactgcccg gcgattttca tctccggcaa cagcacagaa
    16981 ccacactgta tattactgcg tgtttcaata tcgttaccga cggtgacaat attgcgccac
    17041 atatctttta aattacctgc gatggtgatt tcgctaaccg gatactgaat ttcgccattc
    17101 tctacccaga agcccgctgc accacgggaa taatcaccgg taatggcact cacgccctgg
    17161 cccatcagct ccgtcaccac cagcccggtg cccatctctt tgagcatttg ttcgaagctt
    17221 agcccttgtc cggcaatccg ccagttgtga ataccgcccg catgtccggt gcttttcagc
    17281 cccagtttcc gcgccgaata gctggtcagc agccactgag tcaggatgcc atctttaata
    17341 atatcgcgac gctcggtgcg cacaccttcg ctgtcgaatg gcgtagaggc cagccctttc
    17401 agcagatgcg gatgctcttc aatggtcagc cagtccggca gaatttgttt acccagcgaa
    17461 tccagcagga aggtagattt acggtaaacc gatccacccg ctatcgcccc aaccagatgc
    17521 ccaaaaagcc cggttgccac ttcattggca aaaatgaccg gcgctttcat ggtggagagt
    17581 ttacgcggtg acagacgcga taaagtgcgg cgagcacagt cggccccgac ccactctggc
    17641 gtttgcagat cgctcatcgc acgaccaatg gtgtaggcgt aatcacgctc catatcgcca
    17701 ttttcttcgg caattacaca gctggagagc gaatgacgcg tagagcagta accctgcaac
    17761 atgccgtggc tgttgccaaa aactttgaca ccgtagtggc tgttaaagct gccaccttcg
    17821 gtattggtga tgcgtttgtc cgcctgcaat gctgcctgtt ctgcgcgggc cgccagttca
    17881 atggcttcat ccggggaaac ttccgcaggg tggaacaagt cgagatccgg tgcgtcaaag
    17941 gccagcagct ctttgtctgc cacgccggca cagggatctg gcgaggtata acgggcaata
    18001 tctagcgccg cctgtacggt gcgggcaatg gcctgcgggc ttaaatcggt agacgatgcg
    18061 ctacctttgc ggttctggtg ataaacagtg atccccaacg cgccatcgct attgaattca
    18121 acattctcca cttcaccata acgcgtgctt acgctaatgc cggtggtctt gctgacggca
    18181 acttctgcac cgtccgattt gcctgaggcc aactccagcg cagtcgaaac tgcttcttcc
    18241 agaatcttgc gctgcgcttc aacttgagag attactttca ttgcaagtgc cataatgtag
    18301 agagtttctc tgaagtctaa cagagaaccg tttttcagtg cgcatcttaa ctggtaacat
    18361 tagcctcttt ttttaaggag cctgagatga ctaagcagcc cgaagactgg ctcgacgacg
    18421 ttcccggtga tgacatcgaa gacgaagacg atgaaattat ctgggtcagt aaaagtgaaa
    18481 ttaaacgcga tgccgaggag ctaaaacgcc ttggcgcgga aattgttgat ctggggaaaa
    18541 acgcgctgga taagatcccg ttagatgcgg atctgcgcgc ggctattgag ctcgcccagc
    18601 gtattaagat ggaaggtcgc cgccgccagt tgcagctcat cggtaagatg ctgcgccagc
    18661 gcgatgttga gcctattcgt caggcgctgg ataagctgaa aaaccgccac aaccagcaag
    18721 tggtgttgtt ccataaactg gaaaacctgc gcgatcgtct gatcgagcaa ggtgatgacg
    18781 ccatcgctga agtattgaat ctgtggcccg atgccgatcg tcagcaactg cgtactctga
    18841 tccgtaacgc gaagaaagag aaagaaggga ataagccgcc gaaatccgcg cgccagattt
    18901 tccagtatct gcgtgagtta gcggaaaacg aagggtaatt ttcgttgttg atgtgagtat
    18961 ggtttgccgg atgcggtgca cctgtaggcc tgataagacg catcaagcgt cgcatcaggc
    19021 actatgctca aatgccggat gcggcgtgaa cgccttatcc ggcctacaca cactctatca
    19081 ggctgaggct gtgaattact gcgcggcttc cgctttcttc gctaacccat ccagcagttt
    19141 ctgatggatc ccaccaaaac cgccgttact catcaccaga atgtgatcgc ccggctgagc
    19201 ggttttcacc accatatctg ccagcgtatc cacatcgccg ctccagtgtg caggctgaac
    19261 gcaggcttct gccacttctg ccacctgcca cggaatatgc gccggttgca gcaggaagac
    19321 ttcatcggca cgacctaatg aaggtgccag atcgtctttg cagatcccca ttttcatggt
    19381 gtttgagcgc ggttccagca cggcaataat gcgcgccgta ccaccaactt tgccacgcag
    19441 cgccgcaagc gttgccagaa tcgccgtcgg gtgatgggca aaatcgtcat ataccgtcac
    19501 accattcgct tcaccacgca actccagacg gcgacgagca ttaataaacg aacccagcgc
    19561 gttagcggca tctgccggcg ctacaccaac atggcgagcc gccgcaatcg ccatcaggcc
    19621 attgtgcata ttatgttcgc ctaccagcga ccatttcact tcgcccactt tttcgccatc
    19681 cagcaagact tcccattcgg aagcatcggt ggtcagcttt ttcgcctgcc agtgaccttg
    19741 ctcgcccacc agctcctgct cgctccagca gcccatcgcc atggtctgtt tcaggttgat
    19801 gtcattttcc ggccagataa tacggccctg ccccggaacg atacgcacca gatggtggaa
    19861 ctgtttctgg atcgctttca ggtcgtcaaa gatatcggcg tgatcgaact caaggttgtt
    19921 gaggatcagc gtacgcgggc agtaatggac aaatttagag cgtttgtcga agaaggcgca
    19981 gtcatactca tccgcttcga taacaaagaa gtcgctttcg cccagacgcg ccgaaacctc
    20041 aaagttcccc ggcacaccgc cgatcacaaa tcccggtttg taaccgcact gttccagaat
    20101 ccaggttgcc attcccgcgg tggtggtttt gccatgtgta ccggcaacgg ccagcaccca
    20161 gcggtcgcgc agcacaaaat cgtgcagcca ctgcggacct gacatataag ggatgttttt
    20221 ttccagtacc gcttccacac acggatttcc acgggtcatg gcgttaccaa taatcaccag
    20281 atccggctgt ggatcgagct ggctggcatc gtaaccctga atcagttcaa tgccttgctt
    20341 ctcaagtaag gtgctcatcg gcggatacac attggcgtcc gaacccgtta cttcatggcc
    20401 taactggcgc gccagcatcg ccagaccgcc cataaacgtg ccacaaattc ctaaaatatg
    20461 aatgcgcata cgtcactatc cttttttaat ctggcgctca ttttacgcat atgtcgggca
    20521 agtgagaaac gcatttcagg ataatccgta atttgctggc gcgattcacc tgagcgcaac
    20581 attgggatta tttgttaaga ttgttgcggt cgctttactc cataaacatt gcagggaaag
    20641 ttttatgaaa acgttaggtg aatttattgt cgaaaagcag cacgagtttt ctcatgctac
    20701 cggtgagctc actgctttgc tgtcggcaat aaaactgggc gccaagatta tccatcgcga
    20761 tatcaacaaa gcaggactgg ttgatatcct gggtgccagc ggtgctgaga acgtgcaggg
    20821 cgaggttcag cagaaactcg acctgttcgc taatgagaaa ctgaaagccg cactgaaagc
    20881 acgcgatatc gttgcgggca ttgcctctga agaagaagat gagattgtcg tctttgaagg
    20941 ctgtgaacac gcaaaatacg tggtgctgat ggacccactg gatggctcgt ccaacatcga
    21001 tgttaacgtc tctgtcggta ccattttctc catctaccgc cgcgttacgc ctgttggcac
    21061 gccggtaacg gaagaagatt tcctccagcc tggtaacaaa caggttgcgg caggttacgt
    21121 ggtatacggc tcctctacta tgctggttta caccaccgga tgcggtgttc acgcctttac
    21181 ttatgatcct tcgctgggtg ttttctgcct gtgccaggaa cggatgcgct tcccggagaa
    21241 aggcaaaacc tactccatca acgaaggaaa ctacattaag tttccgaacg gggtgaagaa
    21301 gtacattaaa ttctgccagg aagaagataa atccaccaac cgcccgtata cctcacgtta
    21361 tatcggttca ctggtcgcgg atttccaccg taacctgctg aaaggcggta tttatctcta
    21421 cccaagcacc gccagccacc cggacggtaa actgcgttta ctgtatgagt gcaacccgat
    21481 ggcattcctg gcggaacaag caggcggtaa agcgagcgat ggcaaagagc gtattctgga
    21541 tatcatcccg gaaaccctgc accagcgccg ttcattcttt gtcggcaacg accatatggt
    21601 tgaagatgtc gaacgcttta tccgtgagtt cccggacgcg taatctcctg cgcctctcca
    21661 cacggagagg cgtttttatc gctgcgcaat gttgactctt gtcacaggcg aactctgacg
    21721 attctcccac agcaccgtta atccacgctg taatgctata aaaataaaca acaaaatacc
    21781 gatggcgatt ttcgtccacc atgagctcag cgtgccatca aagtttatgt aagtctgaat
    21841 cagtccctga atcgccacgc caaaaagcgt ccctaatacc gttccaacgc caccactcaa
    21901 aagcgtaccg ccaattacca ctgaggcgat agcatccagt tccacaccta cgcccgccag
    21961 cgcatatccg gcctgggtat aaatcgagaa gacaatcccc gccagtgtcg ccagcccggt
    22021 agagagcata taaatgcgaa tagtggtgct gcgagtggaa atccccatca ggttcgccga
    22081 cgttgcgttg ccgccaatgg cgtacacctg attaccaaaa cgcgtacgat gcgcgaggaa
    22141 tatgccgata accaccaccg ccagcatcag cagccccatc gcacttaagc gaccgccgcc
    22201 agggattttc cacgcaaggc ttgagagcgt gtcataaaca ggatggttta tcgggatcga
    22261 ctcttccgaa acgagatagc tgacgccacg caaaaagaac attcctgcca aagtaatgat
    22321 aaacgccggg atcttcaggg cgtcaatcag aagccccata aatgcgccga aggcacagcc
    22381 catcaccaac accagcggaa acgccagcag cggcgagagg ccgaaatcgc caatcacttt
    22441 tgccagaaac acgccggtaa aggcgatcac cgaaccgacg gagagatcga tcccaccgga
    22501 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcattat cggtcaggat
    22561 attgcagatc actcttgtgg aagcaaaacc gggaaactgg gtcagacagt agatataacc
    22621 caacacaaag acgccgatgg tgatcatcag tggcaaatta cgttttatca cggttacgta
    22681 cccctttaat cagactgata aagcgttgcg actggacaat cagcacgcaa agcaccacca
    22741 ccgctttcac cacctggttc atctccggcg gaaatccaga aagcaaaatt ccggtgttca
    22801 ttccctgaat aatcagcgcc cccaccaccg aaagcagcag gttaaagcgc ccgcccatca
    22861 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aacccggcgt
    22921 tattggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
    22981 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
    23041 ccgcccgaat attgatacca acggcttcga taaacatccc cagtgccgtt ttgcgggtca
    23101 acagccagaa caggagaagc gtcagcaccg caataatgac tggcgttggc aggaacaaca
    23161 gcgatccact gccgaaccag gagagatccg gtgagttaaa tgtgacgatc tgtccggagg
    23221 tgatcagttg cgccacaccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
    23281 gaattttgag gatcgctacc agtatgccgt tccacaatcc tgccaggatg ccagtgccca
    23341 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
    23401 ctccggcgat agccatcacc gcccctacgg agagatcaat cccaccggtg gcgataacca
    23461 gcgtcatgcc aatcgccagt agcgcaacgg gggccgcacg gttaagaatg tcgatagggc
    23521 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggcc accaggctat
    23581 cgaccagcag caccagcaac agtgccgcca gctgcggcat tcctgttggc cagcgaaaac
    23641 gccttttcgg cggcgtagtg tccgggagag attgaggcat cacactattc tccttacgcc
    23701 gcaatggcgt tcatgatcgc cggaacggaa agctctgcca gcgggatctc cgccacctgt
    23761 ttgcgatcgc gcatgataat cacccggtcg gcatagccca ccagctcttc cagttcagag
    23821 gagatcacca gcagcgccag accgtcagcg cacagcgttt caatcaggcg gatgatttca
    23881 gcgtgggcac caacatcaat gccgcgggtt ggctcgtcga ggatcaaaaa ttgcggacga
    23941 gtcagtagcc aacgtgaaag caacactttt tgctgattgc cgccagagag aaattcaatc
    24001 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
    24061 tgctgttctt tgcgggaaat cggtcgtagc cagccgcgct gggcctggag agcgaggatg
    24121 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
    24181 aagccgatac ccagtaccga agcctgatgt ggcgatcgca gggtttgctg tttgcctttg
    24241 atcaacgccg tgccgctgtc agcaggtttg atcccgaaga tcacttcggc ggtttcggta
    24301 cgtccggatc ccagcaagcc tgccaggcca acgatctcgc cggggcgtac ttcgagatca
    24361 aacggtgcga tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
    24421 agcaatgttc tcccggcacg ctgaagcgcg tgggtgtcca gttcacgccc cagcatcatt
    24481 tttaccagtt cgatctgcgg tagctcgcgg gtttcccgac agcccacaaa actgccgtta
    24541 cgtaagacgg tgatccgatc gctaacctga taaacctgat cgagaaaatg agtgacgaag
    24601 atcaggctga cgccgcgatc gcgaagctga cgcatcaggc caaacagtaa ctctacttcc
    24661 tgagtgtcga gactggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
    24721 gcccggcaaa tggcgacgat ttgctgcatc gcgactgaaa agcggttgag cggttcgcgc
    24781 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
    24841 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcgaccgac
    24901 atattgggta gcaggttgac ttcctgatag acggtgccga tgccgagttg ttgtgcgtgg
    24961 gcggtgtttt tcggtgagat agcctggcct tccagccaga tagtgccgcg atcggcgtgg
    25021 tatacaccgg ttaatgcttt gattagcgtc gattttcccg ccccgttttc accgagcagc
    25081 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacaccgggg
    25141 aaaaatttac ttaatccttc ggtgcggagg atttcctggt gttggtcggt cgtcatggtt
    25201 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
    25261 gcttcagcgc acaaccgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
    25321 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
    25381 atcaggcaga taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
    25441 tttatatttc tccagcgcgt cgaaggcggg acctgccata ttcggcgtca gttcaacact
    25501 ggcgttcgct tcgccatcaa tcatcgcttt gtagatgtcc ggcacaccgt cgatagagcc
    25561 tgtcaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
    25621 caccatgtca tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
    25681 gataaagctc tccatcactt ctttgccttt gctgcgggtg aagtcacctg actgcgagcg
    25741 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
    25801 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
    25861 ttcttttacc agccagtcac caatcaactt gccttcgagg atgttgtcgg cggtgacagt
    25921 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcaagcaaga agaccgggat
    25981 ttcggcatct ttcgcctctt ttaataccgg ctcccaaccg gtcgctacca ctggagcgat
    26041 aaagatcgca tccacccctt gcgcgacgaa ggaacgtacc gctttaatct ggttttcctg
    26101 cttttgctga ccatcggcaa ttttcaacgt aattccgcgc ttttcggctt cacttttcgc
    26161 cacattggtt tctgcggcgc gccagccgga ttccgacccg acctgcgaaa atccaacggt
    26221 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
    26281 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
    26341 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
    26401 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
    26461 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
    26521 cgaaaacaag cgaagacatt cggcgcgagt tggctataat actcggcact tgtttgccac
    26581 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
    26641 gaagacatct acgttgttat tgagatcccg gctaacgcag atccaatcaa atacgaaatc
    26701 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
    26761 tgcaactacg gttacatcaa ccacactctg tctctggacg gtgacccggt tgacgtactg
    26821 gtcccgactc cgtacccgct gcagccgggt tctgtgatcc gctgccgtcc ggttggcgta
    26881 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactga ttgctgttcc gcacaccaag
    26941 ctgagcaaag aatacgatca cattaaagac gttaacgatc tgccagagct gctgaaagcg
    27001 caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggttaaagtt
    27061 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgcc
    27121 aagaataaat aagttcttct ggcgcaataa ccctgaacac cgggcttcgg ttagtaaggg
    27181 tttttttatg cccgcaattt ataaactctc tactccacta ttattatttt cagcagttga
    27241 aaggcttgaa cggtaagaac aagctaaact cgaccaccat tttgctgttc atagccactt
    27301 gctggaagtt agccggcctc actcatactc accgttcaaa gcgtgtcccc gttttacccc
    27361 tgtacatttg ttaacgccag cagagttttt tatgcgattg cagatatgga aagcggcccg
    27421 gagagaaaag caaaagatgg gaaagttaca aaaaaaatcg cgagatacat cgcataaaac
    27481 aacgccaccc gaaggtggca taagggggta gcatgtaccg caccacccct tgatatatca
    27541 aatagttacg agttcaaagt agttcggaat cgtgccattt agtccccgtt tatgggcaaa
    27601 atctggacaa ctccgcaacc ggattgaacc ttaccgcgtc ctggagataa tcaggtgcaa
    27661 agtgcgcata ggtcattgtc tgctgaattg tgctgtgccc taaaatacgc tgaagggtaa
    27721 taatgttccc tccatttatc atgaaatgag ttgcgaatgt gtgccgtaaa acatgcacag
    27781 actgaccgac tggtaaatca ggccgcacag tccttattgc acttctgact atcgcataac
    27841 tggggttcat taatcttccc gtcgctttga ctttaatcag agattcaaga tctttagcta
    27901 tgggaataac ccgccgcgat ccattctttg tctccatgaa aataaccttc ccaccaatta
    27961 tgtgttcacc tttaatattg gcaacttcac cccagcgccc gcctgtagcc aggcttaaca
    28021 atacagcctt aagattatcg ccagataaaa gtgcggttag tctgggaata tcatcatgtg
    28081 caagataagc catttccggc tgattaattg ttagcttgcg tatctcgtga aacgggttat
    28141 cacccacaaa ctcttcaaca tcaatcagct tttcaaagaa accgctcatc attgcacaat
    28201 aacgatttac cgttgactgc ttaattccgc gattcattaa ttcaaggcga tatctgataa
    28261 tattttttcg ggttaagtgg tcacttctgt taataccaag ctcttgcata tcacgaagaa
    28321 ccgcttcaat tctgacccga ttcatcgcgc cataacgatg attcttaccg tggtacaccc
    28381 accacagttc cagaagatcg cttaacgctt tacgctcaat aggtttttcg acccagtcct
    28441 tatcgtgata atgcgtcaga acatggcgtt catacatcgc cgcttcacca cgggtaggaa
    28501 atcgccgccg aaatcttctt ccttcagatc cagcaggccg cacgtccaca cagtatttgc
    28561 catcagcgag cttcttaatc gacataagga agccccccaa tgaaaacatc atcttgatca
    28621 caattttgag aaataaaggc cagatggacc gttaaccagt cttcttgccg gagtggggtg
    28681 attccgattc gcttcgccca atgtgtgcga gagccggtac cacctgcccg gcctttggtt
    28741 cgacctcatc gaacaaaaac cagtcacggt atttacggaa gacgggagac ttgagcagct
    28801 tttttgtgga ttcaagattt ggctcatttc tcccctgttc atattgacgc agggccgcta
    28861 ccgtgagccc tgtaatttca gccatttttt cttgtgtaag catttcagac ttacgcatga
    28921 ttctaatttt ttcacctacg ttagttgaca tagtgagtta actctcttag tttatacatc
    28981 aacaaacaca cctagagaca atctaggcgg ctctaactgg tgctaaatag aaatagcacc
    29041 aataacggag gataccagat gcaagaagtc actcaaaacg cgcctgaatc gcctgccgaa
    29101 acacagtgcg atcagttgac agatgcgcca gaaaccgcac accaaaagac cgggcgcaaa
    29161 gcccctgaac gccaggaaat caggctttca caaaaccctt ctcatctcct gtcaaaagaa
    29221 gggtttgcga tgtatatcgg caagaccgtt gacgccatcg tgagcatggc aaaagcaggg
    29281 aaattacccg ctgtgtacat ggcggatccc ctcaaacctg gcggaaacgc cgaactttac
    29341 atcaaccgga aagcatggga tgaagcctgc gatcgactca ctgaaaacgc accgcaagaa
    29401 tggcacgaat gggaaaaccg acttttttta tttaagccag cagctggttc acggaataaa
    29461 cgcatcagta aaaaagctgc gtgatataga actggcaatc attggagggg caagatgatt
    29521 aaacacacgg aaatcagaca aagaataaaa ataagcgacg acgcttcagt aataatctgt
    29581 aatcagaact tagatgccat tgttcgggct ggcaacgatt tgtatttcca ttacccgacc
    29641 cattgcatcg ttgtgacatt tgaaacagaa gagtccttaa aaaatgcaat ggagcagaca
    29701 ggatttcaga atgaaacagt aataagaaaa gacaactggg cataaatgcc aatacaccgt
    29761 tatttcaatt cttttaactt aagcgggttt ttccgatatg acacagcgac gcaacttccg
    29821 cactggcaca gaac
//
LOCUS       DAZKGF010000044        29273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZKGF010000044 DAZKGF010000000
VERSION     DAZKGF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.044"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..195
                     /locus_tag="ACHOQV_001874"
     CDS             <1..195
                     /locus_tag="ACHOQV_001874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630258.1"
                     /translation="NCRIVTLPAIPLAVTLPPALLESQYSITIMSPYIRKLISAITLS
                     HQRLYVERVLKIKLNQCIGS"
     gene            395..1534
                     /gene="wza"
                     /locus_tag="ACHOQV_001875"
     CDS             395..1534
                     /gene="wza"
                     /locus_tag="ACHOQV_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFP9630259.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1540..1983
                     /gene="wzb"
                     /locus_tag="ACHOQV_001876"
     CDS             1540..1983
                     /gene="wzb"
                     /locus_tag="ACHOQV_001876"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFP9630260.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFVAVYTLLERSARQWAQALNAEQV"
     gene            1986..4148
                     /gene="wzc"
                     /locus_tag="ACHOQV_001877"
     CDS             1986..4148
                     /gene="wzc"
                     /locus_tag="ACHOQV_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFP9630261.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGEITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4326..5165
                     /gene="wcaA"
                     /locus_tag="ACHOQV_001878"
     CDS             4326..5165
                     /gene="wcaA"
                     /locus_tag="ACHOQV_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFP9630262.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5168..5656
                     /gene="wcaB"
                     /locus_tag="ACHOQV_001879"
     CDS             5168..5656
                     /gene="wcaB"
                     /locus_tag="ACHOQV_001879"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFP9630263.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5653..6870
                     /gene="wcaC"
                     /locus_tag="ACHOQV_001880"
     CDS             5653..6870
                     /gene="wcaC"
                     /locus_tag="ACHOQV_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFP9630264.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQRVAGKRQLFSEMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQSSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6845..8062
                     /gene="wcaD"
                     /locus_tag="ACHOQV_001881"
     CDS             6845..8062
                     /gene="wcaD"
                     /locus_tag="ACHOQV_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFP9630265.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8073..8819
                     /gene="wcaE"
                     /locus_tag="ACHOQV_001882"
     CDS             8073..8819
                     /gene="wcaE"
                     /locus_tag="ACHOQV_001882"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFP9630266.1"
                     /translation="MLLSIITVAFRNLAGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAHGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8835..9383
                     /gene="wcaF"
                     /locus_tag="ACHOQV_001883"
     CDS             8835..9383
                     /gene="wcaF"
                     /locus_tag="ACHOQV_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFP9630267.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9410..10531
                     /gene="gmd"
                     /locus_tag="ACHOQV_001884"
     CDS             9410..10531
                     /gene="gmd"
                     /locus_tag="ACHOQV_001884"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP9630268.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYDMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10534..11499
                     /gene="fcl"
                     /locus_tag="ACHOQV_001885"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10534..11499
                     /gene="fcl"
                     /locus_tag="ACHOQV_001885"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFP9630269.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11502..11981
                     /gene="gmm"
                     /locus_tag="ACHOQV_001886"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11502..11981
                     /gene="gmm"
                     /locus_tag="ACHOQV_001886"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP9630270.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVVEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11978..13201
                     /gene="wcaI"
                     /locus_tag="ACHOQV_001887"
     CDS             11978..13201
                     /gene="wcaI"
                     /locus_tag="ACHOQV_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFP9630271.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWQPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVANADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13204..14640
                     /gene="cpsB"
                     /locus_tag="ACHOQV_001888"
     CDS             13204..14640
                     /gene="cpsB"
                     /locus_tag="ACHOQV_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFP9630272.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRTAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAIDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14833..16203
                     /gene="cpsG"
                     /locus_tag="ACHOQV_001889"
                     /gene_synonym="manB"
     CDS             14833..16203
                     /gene="cpsG"
                     /locus_tag="ACHOQV_001889"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP9630273.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     NNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLADPVEAING
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16258..17652
                     /gene="wcaJ"
                     /locus_tag="ACHOQV_001890"
     CDS             16258..17652
                     /gene="wcaJ"
                     /locus_tag="ACHOQV_001890"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFP9630274.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17654..19132
                     /gene="wzxC"
                     /locus_tag="ACHOQV_001891"
     CDS             17654..19132
                     /gene="wzxC"
                     /locus_tag="ACHOQV_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFP9630275.1"
                     /translation="MNLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDTI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLIVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19793..21073
                     /gene="wcaK"
                     /locus_tag="ACHOQV_001892"
     CDS             19793..21073
                     /gene="wcaK"
                     /locus_tag="ACHOQV_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFP9630276.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHIEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            21070..22290
                     /gene="wcaL"
                     /locus_tag="ACHOQV_001893"
     CDS             21070..22290
                     /gene="wcaL"
                     /locus_tag="ACHOQV_001893"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFP9630277.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIIALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLCGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVIEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVIKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            22301..23695
                     /gene="wcaM"
                     /locus_tag="ACHOQV_001894"
     CDS             22301..23695
                     /gene="wcaM"
                     /locus_tag="ACHOQV_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFP9630278.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCIIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            23853..24848
                     /locus_tag="ACHOQV_001895"
     CDS             23853..24848
                     /locus_tag="ACHOQV_001895"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFP9630279.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLTAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            25091..25984
                     /gene="galF"
                     /locus_tag="ACHOQV_001896"
     CDS             25091..25984
                     /gene="galF"
                     /locus_tag="ACHOQV_001896"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFP9630280.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            26356..27441
                     /gene="rfbB"
                     /locus_tag="ACHOQV_001897"
     CDS             26356..27441
                     /gene="rfbB"
                     /locus_tag="ACHOQV_001897"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9630281.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            27441..28340
                     /gene="rfbD"
                     /locus_tag="ACHOQV_001898"
     CDS             27441..28340
                     /gene="rfbD"
                     /locus_tag="ACHOQV_001898"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFP9630282.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEQCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVATTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTVI"
     gene            28398..>29273
                     /gene="rfbA"
                     /locus_tag="ACHOQV_001899"
     CDS             28398..>29273
                     /gene="rfbA"
                     /locus_tag="ACHOQV_001899"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9630283.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFHQLLGDGGQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKKGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGGLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLRMIKGY"
BASE COUNT         7297 a         7107 c         7861 g         7008 t
ORIGIN      
        1 aattgccgaa ttgttacctt gcctgctatt ccgttagctg taacacttcc tcctgcatta
       61 ttggagagcc aatattcaat taccataatg tcaccttata ttcggaagtt aatttccgca
      121 ataacccttt ctcatcaacg actgtatgtt gagagggttt taaaaattaa gctaaatcag
      181 tgtattggta gctaaaaagc caggggcggt agcgtgtctg gatgcctgaa agaccagtct
      241 gaattattct gtcaatagcc tgcggatgaa atcaattttt ttaggactga tgccagttaa
      301 atttttattc aattaactgc aaggtaaatg tgcatggcga cattgctatt aatagtgcac
      361 aggataatta ctctgccaaa gtgataaata aacaatgatg aaatccaaaa tgaaattgat
      421 gccattattg gtgtcagtaa ccttgataag cggttgcaca gtacttccgg gcagcaatat
      481 gtcgacgatg ggtaaagacg tcatcaaaca gcaggacgct gatttcgatc tcgacaaaat
      541 ggtgaatgtt tatccgttga ccccgcgcct gattgatcaa ttacgcccac gcccgaatgt
      601 agcgcgcccc aatatgacgc tggaaagtga gatcgcgaat tatcagtatc gcgtcgggcc
      661 gggggacgtt cttaatgtca ccgtctggga tcacccggaa ctcaccacgc cagccggtca
      721 gtaccgcagc tccagcgaca ccggcaactg ggtacagcct gacggcacca tgttctaccc
      781 gtatatcggc aaggttcacg tcgtggggaa aacgctcgct gaaatccgca gtgatattac
      841 cggtcgctta gcgacgtaca tcgctgaccc gcaggtggac gttaatatcg ccgccttccg
      901 ctcgcaaaaa gcctatatct caggtcaggt gaataaatcc ggtcaacagg cgattaccaa
      961 cgtaccactg accattctcg atgccatcaa cgccgcaggt ggcctgaccg acaccgctga
     1021 ctggcgcaac gtggtgctaa cacacaatgg tcgtgaagaa cgcatttctt tgcaggcgct
     1081 gatgcaaaac ggcgacctca atcagaaccg actgctttac cccggcgata ttctctacgt
     1141 gccgcgtaat gatgatctga aagtgtttgt gatgggtgaa gttaaaaaac agagcaccct
     1201 gaaaatggac tttagcggca tgaccctaac tgaagccctg ggtaatgctg aaggcatcga
     1261 catgaccacc tccaacgcca gcggcatctt tgtcattcgt ccgttgaaag gcgagggcgg
     1321 gcgtaacggc aagattgcca atatctacca gctggatatg tccgatgcca catcgctggt
     1381 gatggcgaca gaattccgcc tgcaacctta tgacgtggtg tatgtcacca ccgccccggt
     1441 ttcccgctgg aaccgtctga tcaatcagtt gctgccaact attagcggtg tccgttacat
     1501 gacggataca gccagcgaca ttcataactg gtaatcgtca tgtttaacaa catcttagtt
     1561 gtctgtgtcg gcaatatttg ccgttccccg acggcggaac gcttactgca acgttatcac
     1621 ccggagctga aagtggagtc cgctggactc ggcgcgctgg tcggtaaggg cgctgaccct
     1681 actgctatca gcgtcgccgc agaacatcaa ctgtctctgg aaggtcactg tgcacggcaa
     1741 atcagccgtc gcctgtgtcg caactacgac ctgattttga ccatggaaaa gcgccatatc
     1801 gaacgcttat gcgagatggc accggagatg cgcggcaaag tgatgctgtt tggtcactgg
     1861 gataacgaat gtgaaatccc cgatccgtat cgcaaaagcc gggaaacgtt tgtagcggtg
     1921 tacacattac ttgaacggtc tgcccgccag tgggcgcagg cattgaacgc agagcaggta
     1981 taagaatgac agaaaaagta aaacaacatg ccgctccggt aacgggcagt gatgaaatcg
     2041 atattggtcg cctggtcggc accgtcattg aagcgcgctg gtgggtgatt ggcatcaccg
     2101 ccgtatttgc cctctgtgcc gtggtttaca ccttcttcgc tacgccgata tacagcgccg
     2161 acgcactggt acaaatcgaa caaagcagcg gcaattcgtt agtgcaggat atcggatcgg
     2221 cattagccaa caaaccgcct gcatcggacg ccgagatcca gttgattcgt tcgcgcctgg
     2281 tgcttggtaa aacggtggac gatctcgacc tcgatattgc ggtgagcaaa aacacgttcc
     2341 cgattttcgg tgcgggctgg gatcgcctga tgggacgcca gaacgagacg gtaaaagtga
     2401 ccacctttaa ccgtcctaag gagatggcgg atcaggtgtt tacgcttaat gtgcttgatg
     2461 acaaaaacta cactttgagc agcgatggcg gctttagcgc ccgtgggcaa gcgggccaga
     2521 tgctgaaaaa agaaggcgtc acgctgatgg ttgaagctat tcacgccagc ccgggcagtg
     2581 agtttaccgt caccaaatac tccacgctgg ggatgatcaa tcaactgcaa aacagcctga
     2641 cggtaacgga gaacggcaaa gatgctggcg ttttgagcct gacttatact ggagaagatc
     2701 gcgaacagat ccgcgacatt cttaacagta tcgcccgtaa ctatcaggaa caaaatattg
     2761 agcgcaaatc ggcggaagcg tcgaaaagcc tcgctttcct cgcgcaacag ttaccggaag
     2821 tacgtagccg cctcgatgtt gccgaaaaca aactgaatgc cttccgtcag gataaagatt
     2881 cagttgatct gccgctggaa gcgaaagcgg tgctcgattc gatggtgaac atcgacgcgc
     2941 agttgaacga actgaccttt aaagaagcgg aaatctccaa gctgtacacc aaagttcacc
     3001 cggcgtatcg cacgctgctg gagaaacggc aggcgctgga agacgaaaaa gccaaactta
     3061 atggtcgcgt aacggcgatg ccgaaaaccc agcaggaaat tgtccgtctg acccgcgatg
     3121 tcgagtctgg gcagcaggtc tatatgcaac tgctgaataa agagcaggag ctgaaaatca
     3181 ccgaagccag caccgtcggc gatgtgcgca ttgttgaccc ggcaatcacc cagcctggtg
     3241 tgctaaaacc gaagaaaggg ctgattatcc ttggggcgat tatccttggc ctgatgctct
     3301 ctatcgtggg tgtgctgctg cgctcgttgt ttaatcgcgg tatcgaaagt ccgcaggtgc
     3361 tggaagaaca cggtatcagc gtctatgcca gcatcccgct gtcggagtgg cagaaagcgc
     3421 gcgatagcgt caaaaccatc aaagggatta aacgctataa acagagccag ctactggcgg
     3481 tggggaatcc aaccgatctg gcgattgaag ccatccgtag tctgcgcacc agtctgcact
     3541 tcgcgatgat gcaggcacag aacaatgtgt tgatgatgac cggggttagc ccgtcaatcg
     3601 gtaaaacctt tgtctgcgcc aacctggcgg cggtgatcag tcagaccaat aaacgggtgt
     3661 tgttgatcga ctgcgatatg cgcaaaggct acacccacga gctgttgggc actaataacg
     3721 tcaatggcct gtcggaaatt ctgattggtc agggcgagat tactaccgct gctaaaccga
     3781 cctctattgc caaatttgac ctgatcccgc gcggtcaggt accgccaaat ccttcggaac
     3841 tgttgatgag cgaacgtttt gccgaactgg tgaactgggc gagtaaaaac tacgacctgg
     3901 tgttgattga tacgccgccg attctggcag tgaccgatgc ggcaattgtt ggtcgtcatg
     3961 tcggaaccac gttgatggtg gcgcgttatg cggtcaacac attgaaagaa gtggaaacca
     4021 gtctgagccg ctttgagcaa aacggtattc cggtgaaagg ggtgattctg aactccatct
     4081 tccgtcgcgc cagcgcgtat caggattatg gctattacga atacgaatat aagtcggacg
     4141 cgaaataacg aaatccgctg tcggatgcga tgctgacgca tcttatccga cctacgttca
     4201 cgccgcatca ggcaagcaaa cccgctccgt aggtcggata aggcgttcac gccgcatccg
     4261 gcaacagaac ctgtaaacca gcttataaac cggcagaaca tctcgacact ccgggagaac
     4321 aacctatgaa agacaatccg ctgatttcaa tctatatgcc gacctggaac cgtcaacaac
     4381 tggcgattcg ggcgataaaa tcggtcctgc gccaggacta cagtaactgg gagatgatca
     4441 tcgtggatga ttgctccacc tcctgggaac aactacaaca gtacgtcacc gccctcaacg
     4501 atccgcgtat cacttacatt cacaacgaca ttaacagcgg ggcgtgcgcg gtacgtaacc
     4561 aggcgattat gctggcgcaa ggggaatata tcaccgggat cgatgatgat gatgaatgga
     4621 cacccaaccg tctgagcgtc ttcctcgccc ataaacagca actggtcaca cacgcctttt
     4681 tgtacgctaa cgactacgtt tgccagggcg aagtctattc ccaaccggca agtttgccac
     4741 tgtatcccaa atcgccgtat tcacgccgcc tgttctacaa acgcaatatc attggcaatc
     4801 aggtctttac ctgggcgtgg cgtttcaaag agtgtttgtt cgataccgaa ctgaaagcgg
     4861 cacaggatta cgacattttc ctgcggatgg tggtggagta tggcgaaccg tggaaagtgg
     4921 aagaggcgac gcagatcctg catatcaatc acggcgaaat gcagatcacc tcgtcaccga
     4981 aaaaattctc cggatacttc cacttttacc gtaagcacaa agacaaattt gaccgcgcca
     5041 gcaaaaaata tcagctgttt accctctacc agatccgcaa taaacgcatg acctggcgca
     5101 ccttgttgac gctgctgtcg gtgcgtaacg gcaaacgtct ggctgatggt attcgggggc
     5161 gctaaagatg ctggaagatc tgcgcgccaa cagctggagt ttacgcccgt gctgcatggt
     5221 tcttgcctat cgcgccgctc atttttgttc ggtgtggcgc aaaaagaacg tcctcaacaa
     5281 tctttgggcg gccccgctgc tggtgctgta tcgcattatc accgaatgct ttttcggtta
     5341 tgaaatccag gccgccgcga ccattggccg ccgtttcact atccatcacg gttacgccgt
     5401 agtgatcaat aaaaacgtgg tagcggggga tgatttcacc attcgccacg gcgtcactat
     5461 cggcaatcgt ggtgccgata acatggcatg tccacacatt ggcaacggcg tcgaactcgg
     5521 tgccaacgtc attattcttg gtgatatcac gcttggtaac aacgtcaccg tgggcgcggg
     5581 cagcgtggtg ctcgactctg tcccggacaa cgcgctggtg gtgggagaaa aagcgcgagt
     5641 gaaggtaatt aaatgaatat tctgcaattc aatgtgcgac tggcggaagg cggggcggca
     5701 ggtgtggcgt tagatctcca ccagcgcgcg ctgcaacagg ggctggcgtc acattttgtc
     5761 tacggctacg gcaaaggcgg caaagagagc gtcagccatc aaaactatcc gcaggtcatc
     5821 aaacatacgc cgcggatgac cgcgatggcg aacattgcgt tgtttcgtct gcttaatcgc
     5881 gatctgttcg gcaatttcaa tgagttatat cgcaccatta ctcgcacacc gggtccggtg
     5941 gtcctgcatt ttcatgtgct gcacagctac tggttgaatc ttaagagcgt ggtgcgcttt
     6001 tgcgaaaaag tgaaaaacca taaaccggat gtcactctgg tctggacgtt acacgatcac
     6061 tggagcgtta ccggacgctg cgcctttacc gacggttgcg aaggctggaa aacgggctgc
     6121 cagaaatgcc cgaccttaaa taattatccg ccggtgaaga ttgatcgcgc acaccaacgg
     6181 gtggcgggca aacggcagtt attcagcgag atgctggcgc tgggctgtca gtttatttcc
     6241 cccagccagc atgtggcaga cgctttcaat agtctgtacg gtccagggcg ttgccggatt
     6301 atcaataacg gtattgatat ggcaaccgaa gcgattctgg cggacttgcc tccggtgcgc
     6361 gaaacccagg gcaagccgaa aatcgcggtg gtggcgcatg acctgcgtta cgacggcaaa
     6421 actaaccagc aactggtacg cgagatgatg gcgctgggcg acaaaatcga actgcatact
     6481 ttcggtaagt tctcgccgtt caccgctggt aacgttgtta atcacggctt tgaaactgac
     6541 aaacgtaaac tgatgagcgc gctcaaccag atggatgcgc tggtgttcag ttctcgcgtc
     6601 gataactacc cgctgatttt gtgtgaggcg ttatcgattg gcgtgccggt gattgccacc
     6661 catagcgacg cggcgcggga agtgctgcaa aaatccggcg gtaaaaccgt cagcgaagaa
     6721 gatgtgctgc aactggtgca gtcaagcaaa ccggaaatcg cacaggcgat atttggtacc
     6781 acgctggctg agttcagcca gcgcagccgc gccgcctaca gtggacaaca gatgctggag
     6841 gagtatgtca acttctatca gaatctgtag ctacctgctg ctgccgctga tctacctgct
     6901 ggttaacgtc aaaatcgccc agcttggcga aagttttccc atcaccatcg tcactttttt
     6961 acctgtcttg ttactgctgt ttttagaacg catcagcgtt aaaaaattga tgattgcctt
     7021 agggattggt gcgggactca cggcgtttaa ctacctgttt ggtcagtcgt tggatgccgg
     7081 taaatacgtc acttcaacta tgctgtttgt ctatattgtg atcattattg gcatggtgtg
     7141 gagtattcgc tttaaaacga tttcgcccca caaccaccgc aagatattac gtttctttta
     7201 tctggtggtc gggctggtgg tggtgctggc ggcggtggag atggcgcaaa ttatcctcac
     7261 cggcggtagc agtattatgg agtcgatttc gaaatatctg atttacagca acagctatgt
     7321 gctgaatttc attaaattcg gcggcaagcg cacgacagca ctttatttcg aaccggcatt
     7381 tttcgctctg gcattaatct caatttggct cagcatcaaa cagtttggta tcaaaacgcc
     7441 taaaaccgat gctatgattc ttgcagggat aatattatcc ggatcgtttt caggggttat
     7501 gacctttatc ctgttttatt tgctggagtg ggcatttcaa tatctgaata aagaggcgat
     7561 taagaaaaag ttaccgttag cattgatttc tctggctgta ttcctggttg gtgtggtaat
     7621 cgcgtttcct tatatttcca cccgtctggg cgatttaggt acggaaggat cgtcatcgta
     7681 ttatcgtatt gtcggtccgc tggtgatggt cggttattct ttgacccata ttgacggtgt
     7741 agtcagattt ggctcacttt atgaatatgt cgcatcattc ggaatattta acggtgcgga
     7801 tgtcggaaaa accatagaca atggtttgta tctgctgatt atttattttt cctggttcgc
     7861 agtgttttta tcgctgtggt acatggggaa agtgataaaa atgatgatca acgcttttgg
     7921 tgataaccgc aattttcgcg tgcaattata tctttttact ccggtatcgc tgttttttac
     7981 cggttcgata tttagcccgg aatatgcatt tttaatcgtc tgtccgttta ttttgcgaaa
     8041 agcgttaaat attacgaggt aagaataaga acatgttgct tagcataatc actgtcgcgt
     8101 ttcgtaacct cgcagggata gtcaaaacac atgcctcgtt ggcgcatctg gcgcaggctg
     8161 acgatatcag cttcgaatgg attgttgtcg atggcggttc caatgacggc acccgtgagt
     8221 atctggaaaa tctcaatggt atctataacc tacgctttgt cagcgagcca gataacggta
     8281 tctacgacgc catgaacaaa ggcattgcga tggcgcatgg caagttcgcg ttgtttctca
     8341 actcaggcga tatttttcat caggatgccg caaattttgt ccgtaagtta aaagtgcaaa
     8401 aagataacat gatgatcacc ggcgatgcgc tgctggattt tggcgacggg cataaaatta
     8461 aacgtagcgc caaaccgggt tggtatattt atcacagcct gcccgccagt catcaggcga
     8521 tatttttccc ggtgtccggc ttgaaaaaat ggcgttatga cctggaatat aaagtttctt
     8581 ccgactacgc gctggcagcc aaaatgtata aagccggtta tgcatttaaa aaactcaatg
     8641 gcctggtgtc tgaattttct atgggtgggg tatcaaccac caataatatg gaattgtgtg
     8701 ctgacgccaa aaaagtccaa cggcaaatat tacatgtgcc tggcttctgg gcagaattat
     8761 cctggcattt acgccaacgt actacctcaa agacgaaagc cttatataac aaaagctgaa
     8821 tataagggaa aacgatgcaa gatttaagcg gtttctcggt accgaaaggg ttccggggtg
     8881 gcaacgctat taaagtgcaa ttgtggtggg cagtacaggc aacaatattt gcctggtcgc
     8941 cacaagtatt gtatcgctgg cgggcatttt tattacgttt attcggcgca aaaataggaa
     9001 aaaacgtagt tattcgtccg tcagtaaaaa ttacctatcc gtggaaatta accttaggtg
     9061 attacgcgtg ggtcggcgat gacgtcaatt tatataccct cggtgaaata accattggcg
     9121 cacattcggt gatatcgcaa aaaagttatt tatgcaccgg tagccacgac catgcaagtc
     9181 aacatttcac cattaacgcc acgcctattg tgattggcga gaaatgctgg ctggcaaccg
     9241 atgtctttgt tgccccaggc gtcacgatcg gcaacggcac cgtcgtgggt gcacgaagca
     9301 gtgtttttaa atcgcttccg gcaaatgtgg tttgccgggg gaatcccgca gtggtgatac
     9361 gcgaacgcgt tgaaactgaa taaattcaaa aaatacagag gaataagaca tgtcaaaagt
     9421 cgctctcatc accggtgtaa ccggacaaga cggttcttac ctggcagagt ttctgctgga
     9481 aaaaggttac gaggtgcatg gtattaagcg tcgtgcatcg tcattcaaca ccgagcgcgt
     9541 ggatcacatt tatcaggatc cgcacacctg caacccgaaa ttccatctgc attatggcga
     9601 cctgagtgat acctccaacc tgacgcgtat tttgcgtgaa gtgcagccgg atgaagtgta
     9661 caacctgggc gcaatgagcc acgttgcggt ttcttttgag tcaccggaat ataccgcaga
     9721 cgtcgatgcg atgggtacgc tgcgcctgct ggaggcgatc cgcttcctcg gtctggaaaa
     9781 gaaaacccgt ttctatcagg cttctacttc tgaactgtac ggtctggtgc aggaaattcc
     9841 gcagaaagag accacgccgt tctacccacg ctctccgtat gcggtcgcca aactgtacgc
     9901 ctactggatc accgttaact accgcgaatc ctacgacatg tacgcctgta acggtattct
     9961 cttcaaccat gaatccccgc gccgtggcga gactttcgtt acccgcaaaa tcacccgcgc
    10021 aatcgccaac atcgcccagg ggctggagtc gtgcctgtat ctcggcaata tggattccct
    10081 gcgtgactgg ggccacgcca aagactacgt aaaaatgcag tggatgatgc tgcaacagga
    10141 acaaccggaa gatttcgtta tcgcgaccgg cgttcagtac tccgtacgtc agttcgtgga
    10201 aatggcggca gcacagttgg gcatcaaact gcgctttgaa ggcacgggtg ttgaagagaa
    10261 gggcattgtg gtttccgtca ccgggcatga cgcgccgggc gttaaaccgg gcgatgtgat
    10321 tatcgccgtt gacccgcgtt acttccgtcc ggcggaagtt gaaacgctgc tcggcgaccc
    10381 gaccaaagcg cacgaaaaac tgggctggaa accggaaatc accctcagag agatggtgtc
    10441 tgaaatggtg gctaatgacc tcgaagcggc gaaaaaacac tctctgctga aatctcacgg
    10501 ctacgacgtg gcgatcgcgc tggagtcata agcatgagta aacaacgcat ttttattgct
    10561 ggccatcgcg ggatggtcgg ttccgccatc aggcggcagc tcgaacagcg cggtgatgta
    10621 gagctggtat tacgcacccg cgacgagctg aacctgctgg acagccgcgc ggtgcatgat
    10681 ttctttgcca gcgaaagcat tgaccaggtc tatctggcgg cggcgaaagt gggcggcatt
    10741 gttgccaaca acacgtatcc ggcggatttc atctaccaga acatgatgat tgagagcaac
    10801 atcattcacg ccgcgcatca gaacgacgtg aacaaactgc tgtttctcgg atcgtcctgc
    10861 atctacccga aactggcaaa acagccgatg gcagaaagcg agttgttgca gggcacgctg
    10921 gagccgacta acgagcctta tgctattgcc aaaatcgccg ggatcaaact gtgcgaatct
    10981 tacaatcgcc agtacggacg cgattaccgc tcagtcatgc cgaccaacct gtacgggccg
    11041 catgacaact tccacccgag taactcccat gtgatacctg cattgctgcg tcgcttccac
    11101 gaggcgacgg cacagaatgc gccggacgtg gtggtatggg gcagcggtac accgatgcgt
    11161 gaattcctgc acgtcgatga tatggcggcg gcgagcattc atgtcatgga gctggcgcac
    11221 gaagtctggc tggaaaacac ccagccgatg ctgtcgcaca ttaacgtcgg cacgggcgtt
    11281 gactgcacta tccgcgagct ggcgcaaacc atcgccaaag tggtgggtta caaaggtcgg
    11341 gtggtttttg atgccagtaa accggatggt acaccgcgca aactgctgga tgtgacgcgc
    11401 ctgcatcagc ttggctggta tcacgaaatc tcactggaag cggggcttgc cagcacttac
    11461 cagtggttcc ttgagaatca agaccgcttt cgggggtaat gatgttttta cgtcaggaag
    11521 actttgccac ggtagtgcgc tccacaccgc ttgtctctct cgactttatt gtcgagaaca
    11581 gtcgcggcga gtttctgctt ggcaaaagaa ccaaccgccc ggcgcagggt tactggtttg
    11641 tgccgggagg gcgcgtgcag aaagacgaaa cgctggaagc cgcatttgag cggctgacga
    11701 tggcggaact ggggctgcgt ctgccgataa cagcaggcca gttttacggt gtctggcagc
    11761 acttttatga cgataacttc tccggcacgg atttcaccac tcactatgtg gtgctcggtt
    11821 ttcgcttcag agtagtggaa gaagagctgt tactgccgga tgagcagcat gacgattacc
    11881 gctggctgac gccggacgca ctgctcgcca gtgacaatgt tcacgctaac agccgcgcct
    11941 attttctcgc tgagaagcgt gccggagtac ccggattatg aaaatactgg tctacggcat
    12001 taactactcg ccggagttaa ccggcatcgg caaatacacc ggcgagatgg tggaatggct
    12061 tgcggcgcaa ggtcatgagg tgcgggttat taccgcaccg ccttactacc cgcaatggca
    12121 ggtgggcgag aactattccg cctggcggta caaacgagaa gagggggccg ccacggtgtg
    12181 gcgctgcccg ctgtacgtgc caaaacagcc gagcaccctg aaacgcctgt tgcatctcgg
    12241 cagttttgcc gtcagcagtt ttttcccgtt gatggcgcaa cgtcgctggc agccggatcg
    12301 cattattggc gtggtgccaa cgctgttttg cacgccggga atgcgcctgc tggcgaaact
    12361 ctctggggcg cgcacggtgc tccatattca ggattacgaa gtggacgcca tgctggggct
    12421 gggccttgcc ggaaaaggta aaggcggcaa agtggcacag ctggcaacgg cgttcgaacg
    12481 tagcggactg cataacgtcg ataacgtctc cacgatttcg cgttcgatga tgaataaagc
    12541 tatcgaaaaa ggcgtggcgg cggaaaacgt catcttcttc cccaactggt cggaaatcgc
    12601 ccgttttcag catgttgcaa atgccgatgt tgatgccctt cgtaaccagc ttggcctgcc
    12661 ggataacaaa aaaatcattc tttactccgg caatattggt gaaaagcagg ggctggaaaa
    12721 cgttattgaa gctgccgatc gcctgcgcga tgaaccgctg atttttgcca ttgtcgggca
    12781 gggcggcggc aaagcgcggc tggaaaaaat ggcgcagcag cgtggactgc gcaacatgca
    12841 attttttccg ctgcaatcgt atgacgcttt acccgcactg ctgaagatgg gcgattgcca
    12901 tctggtggtg caaaaacgcg gcgcggcaga tgccgtattg ccgtcgaaac tgaccaatat
    12961 tctggcggta ggcggtaacg cggtgattac cgcagaagcc cacaccgaac tgggacagct
    13021 ttgcgaaacc tttccgggca ttgcggtttg cgtggaaccg gaatcggtcg aggcgctggt
    13081 ggcggggatt cgtcaggcgc tcctgctgcc caaacacaac acggtggcac gtgaatatgc
    13141 cgaacgcacg ctcgataaag agaacgtgtt acgtcaattt ataaatgata ttcggggata
    13201 attatggcgc agtcgaaact ctatccagtt gtgatggcag gtggctccgg tagccgctta
    13261 tggccgcttt cccgcgtact ttaccccaag cagtttttat gcctgaaagg cgatctcacc
    13321 atgctgcaaa ccaccatctg ccgcctgaac ggcgtggagt gcgaaagccc ggtggtgatt
    13381 tgcaatgagc agcaccgctt tattgtcgcg gaacagctgc gtcaactgaa caaactcacc
    13441 gagaatatta ttctcgaacc ggcagggcgt aacactgcac ctgccattgc gctggcggcg
    13501 ctggcggcaa aacgtcatag cccggagagc gacccattaa tgctggtgct ggcggcggat
    13561 catgtgattg ccgatgaaga cgcttttcgc accgccgtgc gtaatgccat gccgtatgcc
    13621 gaagcgggca agctggtgac cttcggcatt gtgccggatc tacctgaaac cggatatggc
    13681 tatattcgtc gcggtgaagt gtctgcgggt gagcaggata cggtggcctt tgaagtggcg
    13741 cagtttgtcg aaaaaccgaa tctggaaacc gcgcaggcct atgtggcaag cggcgaatat
    13801 tactggaaca gcggtatgtt tctgttccgc gccggacgct atctcgaaga actgaaaaaa
    13861 tatcgcccgg atattctcga cgcctgtgaa aaagcgatga gcgccatcga tccggatctc
    13921 gattttattc gtgtggatga agaagcgttt ctcgcctgcc cggaagagtc ggtggattac
    13981 gcagtcatgg aacgtacggc agatgctgtt gtggtgccga tggatgcggg ctggagcgat
    14041 gtcggttcct ggtcctcttt atgggagatc agcgcccaca ccgccgaggg caacgtttgc
    14101 cacggcgatg tgattaatca caaaactgaa aacagctatg tgtatgccga atctggcctg
    14161 gtcaccaccg tcggggtgaa agatctggtg gtggtgcaga ccaaagatgc ggtgctgatt
    14221 gccgaccgta acgcggtgca ggatgtgaaa aaagtggtcg agcagatcaa agccgatggt
    14281 cgccatgagc atcgggtgca tcgcgaagtg tatcgtccgt ggggcaaata tgactctatc
    14341 gacgcgggcg accgctacca ggtgaaacgc atcaccgtga aaccgggcga aggcttgtca
    14401 gtacagatgc accatcaccg cgcggaacac tgggtagtgg tcgcgggaac ggcaaaagtc
    14461 actatcgacg gtgatatcaa actccttggt gaaaacgagt ccatttatat tccgctgggg
    14521 gcgacgcatt gcctggaaaa cccggggaaa attccgctcg atttaattga agtgcgctcc
    14581 ggctcttatc ttgaagagga tgatgtggtg cgcttcgcgg atcgctacgg acgggtgtaa
    14641 acgtcgcatc aggcgctgtt tgtcggatgc ggcgtgaacg ccttatccga cctacggttc
    14701 gggtttgtag gcctgataag acgcggcagc gtcgcatcag gcaatgaatg cgaaaccgcg
    14761 atgtaaataa cgacaaaaac aaaattggcc gtttcggtca gggccaacta ttgcctgaaa
    14821 aagggtaacg atatgaaaaa attaacctgc tttaaagcct atgatattcg cgggaaatta
    14881 ggcgaagaac tgaatgaaga tatcgcctgg cgcattggtc gcgcctatgg cgaatttctc
    14941 aaaccgaaaa ccattgtgtt aggtggtgat gcccgcctca ccagcgaaac cttaaaactg
    15001 gcgctggcga aaggtttaca ggatgcgggc gtcgatgtgc tggatattgg tatgtccggc
    15061 accgaagaaa tctatttcgc cacgttccat ctcggcgtgg atggcggcat tgaagttacc
    15121 gccagccata acccgatgga ttacaacggt atgaaactcg tgcgcgaggg ggcccgcccg
    15181 atcagcgggg ataccggact gcgcgacgtc cagcgtctgg cagaagccaa cgactttcct
    15241 cccgttgatg aaaccaaacg cggtcgctat cagcaaatca atctgcgtga cgcttacgtt
    15301 gatcacctgt tcggttatat caacgtcaaa aacctcacgc cgctcaagct ggtgatcaac
    15361 tccgggaacg gcgcagcggg tccggtggtg gacgccattg aagcccgctt taaagccctc
    15421 ggcgcaccag tggaattgat caaagtgcac aacacgccgg acggcaattt ccccaacgga
    15481 attcctaacc cgctgctgcc ggaatgccgc gacgacaccc gcaatgcggt catcaaacac
    15541 ggcgcggata tgggcattgc ctttgatggc gattttgacc gctgttttct gtttgacgaa
    15601 aaagggcagt ttatcgaggg ctactacatt gtcggcctgc tggcagaagc gttcctcgaa
    15661 aataatcccg gcgcgaagat catccacgat ccgcgtctct cctggaacac cgttgatgtg
    15721 gtgactgccg caggcggcac cccggtaatg tcgaaaaccg gacacgcctt tattaaagaa
    15781 cggatgcgca aggaagacgc catctacggt ggcgaaatga gcgcccacca ttacttccgt
    15841 gatttcgctt actgcgacag cggcatgatc ccgtggctgc tggtcgccga actggtgtgc
    15901 ctgaaaggga aaacgctggg cgaactggtg cgtgaccgga tggcagcatt tccggcaagc
    15961 ggtgagatca acagcaaact ggcggaccct gttgaggcga ttaacggcgt cgaacagcat
    16021 tttagccgcg aggcgctggc ggtggatcgc accgatggca tcagcatgac ctttgccgac
    16081 tggcgcttta acctgcgcac ctccaacacc gaaccggtgg tgcgcctgaa tgttgaatcg
    16141 cgcggtgatg tgccgctgat ggaagcgcga acgcgaactc tgctgacgtt gctgaacgag
    16201 taatgtcgga tcttccctta ccccactgcg ggtaaggggc taataacagg aacaacgatg
    16261 acaaatctaa aaaagcgcga gcgagcgaaa accaatgcat cgttaatctc tatggtgcaa
    16321 cgcttttcag atatcaccat catgtttgcc ggactatggc tggtttgcga agtcagcgga
    16381 ctgtcattcc tctacatgca cctgttggtg gcgctgatta cgctggtggt gttccagatg
    16441 ctgggcggca tcaccgattt ttatcgctca tggcgcggtg ttcgggcagc gacagaattt
    16501 gccctgctgc tacaaaactg gaccttaagc gtgattttca gcgccgggct ggtggcgttc
    16561 aacaatgatt tcgacacgca actgaaaatc tggctggcgt ggtatgggct gaccagtatc
    16621 ggactggtgg tttgccgttc gtgtattcgc attggggcgg gctggctgcg taatcatggc
    16681 tataacaagc gcatggtcgc ggtggcgggg gatttagccg ccggacaaat gctgatggag
    16741 agcttccgta atcagccgtg gttagggttt gaagtggtgg gcgtttacca cgacccgaaa
    16801 ccgggtggcg tttctaacga ctgggcgggc aatctgcaac agctggtcga ggatgcgaaa
    16861 gcgggcaaga ttcataacgt ctatatcgcg atgcaaatgt gcgacggcgc acgcgtgaaa
    16921 aaactggtcc atcaattggc ggacaccacc tgttcggtgc tgctgatccc cgatgtcttt
    16981 accttcaaca ttctccattc ccgtatcgaa gagatgaacg gcgtaccggt ggtgccgctg
    17041 tatgacacgc cgctttccgg ggttaaccgc ctgctcaaac gtgcggaaga tattgtgctg
    17101 gcgacgctta tcctgctgct gatctccccg gtgctgtgct gcattgcgct ggcggtgaaa
    17161 ctgagctcac cggggccggt tattttccgc cagactcgct acggcatgga cggcaagccg
    17221 atcaaagtgt ggaagttccg ttccatgaaa gtgatggaga acgacaaagt ggtgacccag
    17281 gcgacgcaga acgatccgcg cgtcaccaaa gtggggaact ttctgcgccg cacctcgctg
    17341 gatgaattgc cgcagtttat caatgtgctg actggcggga tgtcgattgt cgggccacgt
    17401 ccgcacgcgg tggcgcataa cgaacagtat cgccagctca ttgaaggcta catgctgcgc
    17461 cataaagtga aaccgggcat taccggctgg gcgcagatta acggctggcg cggcgaaacc
    17521 gacacactgg agaaaatgga aaaacgcgtc gagttcgacc ttgagtacat ccgcgaatgg
    17581 agcgtctggt tcgatatcaa aatcgttttc ctgacggtat tcaagggctt cgttaacaaa
    17641 gcggcatatt gatatgaact tacgtgaaaa aaccatcagc ggcgcgaagt ggtcggcgat
    17701 tgccacggtg atcatcatcg gcctcgggct ggtgcagatg accgtgctgg cgcggattat
    17761 cgacaaccac cagttcggtt tgcttaccgt gtcgctggtg atcatcgcgc tggcagatac
    17821 gctttctgac ttcggcatcg ctaactcgat tattcagcga aaagaaatca gccaccttga
    17881 actcaccacg ctgtactggc tgaacgtcgg gctggggatc gtggtgtgcg tggcggtgtt
    17941 tttgttgagt gataccattg gcgacgtgct gaataacccg gacctggcac cgttgattaa
    18001 aacattgtcg ctggcgtttg tggtgatccc ccacgggcag cagttccgcg cgttgatgca
    18061 gaaggagctg gagttcaaca aaatcggcat gatcgaaacc agtgcggtgc tggcgggctt
    18121 cacttttacg gtggttagcg cccatttctg gccgctggcg atgaccgcga tcctcggtta
    18181 tctggtcaat agtgcggtaa gaacgctgct gtttggctac tttggccgca aaatttatcg
    18241 ccccggtctg catttctcgc tggcgtcggt ggcaccaaac ttacgttttg gtgcctggct
    18301 gacggcggac agcatcatca actatctcaa taccaacctt tcaacactcg tgctggcgcg
    18361 tattctcggc gcgggcgtgg cagggggata caacctggcg tacaacgtgg ccgttgtgcc
    18421 gccgatgaaa ctgaacccca tcatcacgcg cgtgttgttt ccggcatttg ccaaaattca
    18481 ggacgacacc gaaaagctgc gcgtcaactt ctacaagctg ctgtcggtgg tggggattat
    18541 caactttccg gcgctgctgg ggctgatagt ggtgtcgaat aactttgtac cgctggtatt
    18601 tggtgagaag tggaacagca ttattccggt gctgcaattg ctgtgtgtgg tggggctgct
    18661 gcgctcagtg gggaacccga ttggttcgct gctgatggcg aaagcgcggg tcgatatcag
    18721 ctttaaattc aacgtattca aaacctttct gtttattccg gcgattgtta ttggtgggca
    18781 gatggcgggc gcaatcggcg tcacgcttgg cttcctgctg gtgcagatta tcaacaccat
    18841 tctgagctat ttcgtgatga ttaaaccggt gctcggttcc agttatcgcc agtacatcct
    18901 gagtttgtgg ctgccgtttt atctctcgct gccgacgctg gtggtcagtt atgcgctggg
    18961 cattgtgctg aaagggcaac tggcgctggg gatgctgctg gcggtgcaaa tagccacggg
    19021 cgtgctggcg tttgtggtga tgattgtgct gtcgcgccat ccgctggtgg tggaagtgaa
    19081 gcgtcagttt tgtcgcagcg aaaaaatgaa aatgctttta cgggcggggt gaatggttgt
    19141 tttcggttcc ggcaacggtt gccggatgcg gcgtaaaacg ccttatccgt cctggggggc
    19201 tgggcttgga tcttgtaggc ctgataagac gcgccagcgt cgcatcaggc actgaatgcc
    19261 ggatgcggcg tgaacgcctt atccggccta cggttctgtg ctcggatctt gtaggcctga
    19321 taagacgcgt cagcgtcgca tcaggcactg aatgccggat gcggcgtaaa acgccttatc
    19381 cgtcctacgg ctctgtgctc gggtcttgta ggcctgataa gacgcgtcag cgtcgcatca
    19441 ggcactgaat gccggaagcg gcgtgaacgc cttatccgtc ctacggttct gggcttggat
    19501 cttgtaggcc tgataagagg cgtcagcgtc gcatcaggta ctgaatgccg gatgcggcgt
    19561 gaacgcctta tccgtcctac ggttctgggc ttggatcttg taggcctgat aagacgcgtc
    19621 agcgtcgcat caggcactga atgccggatg cggcgtgaac gccttatccg tcctacggtt
    19681 ctgtgctcgg atcttgtagg cctgataaga cgcgttagcg tcgcatcagg caaaaaacgg
    19741 caacgaacac aacaaacaca gcctcacctg gcatcaaatt tatgaggaaa aaatgaaatt
    19801 acttattctg ggcaaccaca cttgcggcaa tcgtggcgat agcgccatcc tgcgtggctt
    19861 acttgatgcc atcaatatcc tcaatccaca tgccgaagtg gacgtgatga gccgctatcc
    19921 ggtcagttct tcctggctgc tcaaccgccc ggtaatgggc gatccgctgt tcctgcaaat
    19981 gaaacaacac aacagcgcgg cgggcgttgt cgggcgtgtt aaaaaagtcc tccgtcgccg
    20041 ctaccagcac caggtattgc tctcacgcgt caccgacact ggcaagctgc gtaatatcgc
    20101 catcgcccag ggattcactg acttcgtgcg actgctgtca ggttacgacg ccattattca
    20161 ggtcggcgga tcgttttttg tcgatctcta cggcgtgccg cagtttgaac atgcgctttg
    20221 cactttcatg gcgaaaaagc cgctgtttat gattggtcac agcgtcggcc ccttccagga
    20281 tgagcaattt aaccaactgg cgaactatgt ctttggtcac tgcgacgcgc tgatcctgcg
    20341 cgaatcggtc agccttgatt tgatgaaacg cagcaatatc accactgcaa aagtggaaca
    20401 tggcgtcgat accgcgtggc tggtcgatca ccacattgaa gacttcaccg ccagctatgc
    20461 tgttcaacac tggctggatg ttgccgcaca acagaaaacg gtggcaatta ccctgcgcga
    20521 actggcaccg ttcgacaaac gtctcggcac cactcaacaa gcgtatgaaa aagcctttgc
    20581 cggggtggtc aatcgcattc tcgatgaagg gtatcaggtg attgcgctct ccacctgtac
    20641 gggcatcgac agttataaca aagacgaccg catggtggcg ctcaatctgc gccagcacat
    20701 cagcgatcct gcccgttatc acgtagtgat ggatgaactc aacgatctgg aaatgggcaa
    20761 aattctgggg gcctgtgaac ttaccgtcgg tacgcgcctg cactctgcta ttatctcgat
    20821 gaactttgcc actccggcga ttgccatcaa ctacgaacat aaatccgccg ggatcatgca
    20881 acaactgggg ctaccagaaa tggcgattga tatccgtcat ttattagacg gcagcctgca
    20941 agcgatggtt gcggatacct taggccagct tccggcactg aacacgcgac ttaacgaagc
    21001 cgtcagtcgc gaacgtcaga cgggaatgca gatggtgcaa tctgtgcttg aacgcatcgg
    21061 ggaggtgaaa tgaaggtcgg cttcttttta ctgaaatttc cgttgtcgtc ggaaaccttc
    21121 gttcttaacc aaatcactgc gtttattgat atgggatttg aggtggagat tatcgcgctg
    21181 caaaaaggcg acacacaaaa cacccacgcg gcatggacga aatacaacct tgctgccaga
    21241 acccgctggt tacaggacga acctacgggc aaagtggcga aactgcgcca ccgcgccagc
    21301 cagaccttat gcggcattca tcgtaaaaat acctggcagg cgctcaacct caaacgctat
    21361 ggcgccgagt cgcggaacct gattttgtct gctatttgcg gtcaggtcgc aacaccgttt
    21421 catgccgatg tctttatcgc tcactttggt ccggcagggg taaccgcagc aaaactccgc
    21481 gaactgggtg tcattcgcgg caaaattgcc actatcttcc acggtattga tatctccagt
    21541 cgggaggtgc tcaaccatta cactcccgaa tatcagcaac tgttccgccg tggcgacctg
    21601 atgttaccga taagcgatct gtgggccgga aggctgcaaa aaatgggctg cccgagggaa
    21661 aaaatcgccg tatcgcgtat gggcgtggac atgacgcgct ttagcccgcg tccggtgaaa
    21721 gcgcctgcaa cgccgctgga aatcatttcc gtcgcacgct taaccgagaa aaaaggcctg
    21781 catgtggcga tcgaagcctg ccgtcagttg aaagagcagg gcgtggcatt tcgctatcgc
    21841 atcctcggca ttggcccgtg ggaacgacgc ctgcgcaccc tcatcgaaca atatcaactg
    21901 gaagatgtga tagagatgcc gggctttaaa ccgagccatg aagtgaaggc gatgctcgac
    21961 gacgcggatg tcttcctgtt gccatcggtt acaggtgcgg acggcgatat ggaaggtatt
    22021 ccggtggcac taatggaagc gatggcggtc ggcattccgg tggtttctac tctgcatagc
    22081 ggaataccgg aactggtgga ggccgataaa tccggctggc tggtgcctga gaacgatgct
    22141 cgcgcactgg cgcaacgact ggcggcgttt agccaactgg acaccgacga actggctccg
    22201 gttatcaaac gtgctcgcga aaaagtcgaa cacgatttta accagcaggt gatcaatcga
    22261 gaactcgcca gcttgctgca ggctttatag aggagaacgt atgccattta aaaaactctc
    22321 ccgacgcacc ttcctgacgg caagctcggc gcttgccttc ctccataccc ccttcgcccg
    22381 cgcacttcca gcccgacaaa gcgttaatat caacgattac aatccgcacg actggatcgc
    22441 ctcatttaaa caagccttca gtgaagggca aacggtcgtc gtgcctgccg gattggtttg
    22501 tgacaatatc aacaccggca tcttcattcc tcctggaaaa accttacaca tccttggaag
    22561 cctgcgcggc aacggcagag ggcgatttgt cttacaggac ggcagccagg tgacagggga
    22621 ggagggcggc agtatgcata acatcaccct ggatgtgcgc ggttctgact gcatcatcaa
    22681 agggttggcg atgagcggtt ttggcccggt aacgcagatt tatatcggcg gcaaaaataa
    22741 acgcgtcatg cgcaacttga ccatcgataa cctgaccgtc agccacgcta attacgccat
    22801 tttacgccag ggatttcata accagattat cggtgccaac atcaccaact gtaagttcag
    22861 cgacttacag ggcgacgcca ttgaatggaa tgtggcgatt aacgaccgcg atattttgat
    22921 atccgaccac gttattgagc gcatcaactg taccaacggc aaaatcaact ggggcatcgg
    22981 cataggcctt gcgggaagca cttacgataa caactatccg gaagaccagg cagtgaaaaa
    23041 ctttgtcgtg gcgaatatca cgggatcaga ttgtcgacag ttgatacatg ttgaaaatgg
    23101 taaacatttt gttattcgta atatcaaagc ccgcaatatc acgccggatt tcagtaagaa
    23161 agcaggtatt gataacgcca cggtcgctat ttacggttgt gacaatttcg tgattgataa
    23221 tattgaaatg attaatagcg ccgggatgtt aattggctat ggggtaatta aaggcaaata
    23281 tctctcgata ccgcaaaatt tccgagtgaa taatattcaa ctggataaca ctcaccttgc
    23341 ttataaattg cgcggcatcc aaatctccgc cgggaatgct gtctcctttg tggcgctgac
    23401 taacattgag atgaagcgtg cctcgctgga gcttcacaat aaaccgcaac atctttttat
    23461 gcgaaatatc aaagtgatgc aggaatcctc tgttggaccc gcattgagca tgaacttcga
    23521 catgcgcaaa gacgttcgcg gcgtctttat ggcgaaaaaa gaaacactgc tgtctcttgc
    23581 aaatgttcat gcgatgaatg aaagagggca aagctccgtc gatatcgaca gggttaatca
    23641 ccatattgtt aatgtggaaa agattaactt tagattgcca gaacggaggg agtagatttg
    23701 cgaccattca taaacttact ggatataatg ttctaaaaga ttataatcct cacactaact
    23761 gttaaagctg gggctgggca gcattgacta ctcagggaag gtactcatct ttatcttgtg
    23821 tttcttacgt aacaaccaga ctaaagtgat ttatgaacga taacgttttg ctcataggag
    23881 cttccggatt cgtaggaacc cgactacttg aaacggcaat tgctgacttt aatatcaaga
    23941 acctggacaa acagcagagc cacttttatc cagaaatcac acagattggc gatgttcgtg
    24001 atcaacaggc actcgaccag gcgttagctg gttttgacac tgttgtgcta ctggcagcgg
    24061 aacaccgcga tgacgtcagc cctacttctc tctattatga tgtcaacgtt cagggtaccc
    24121 gcaatgtgct gacggccatg gaaaaaaatg gcgttaaaaa tatcatcttt accagttccg
    24181 ttgctgttta tggtttgaac aaacacaacc ctgacgaaaa ccatccacac gatcctttca
    24241 accactacgg caaaagtaag tggcaggcag aggaagtgct gcgtgaatgg tataacaaag
    24301 caccaacaga acgttcatta accatcatcc gtcctaccgt tatcttcggt gaacgcaacc
    24361 gcggtaacgt ctataacttg ctgaaacaga tcgctggcgg caagtttatg atggtgggcg
    24421 cagggactaa ctataagtcc atggcttatg ttggaaacat tgttgagttt atcaagtaca
    24481 aactgaagaa tgttgccgca ggttatgagg tttataatta cgttgataag ccagatctga
    24541 acatgaacca gttggttgct gaagttgaac aaagtctgaa caaaaagatc ccttctatgc
    24601 acttgcctta cccactagga atgttgggtg gatattgctt tgatatcctg agcaaaatta
    24661 cgggcaaaaa atacgctgtc agctctgtgc gcgtgaaaaa attctgcgca acaacacagt
    24721 ttgacgcaac gaaagtgcat tcttcaggtt ttgtggcacc gtatacgctg tcgcaaggtc
    24781 tggatcgaac actgcagtat gaattcgttc atgccaaaaa agacgacata acgtttgttt
    24841 ctgagtaatg cagacgagtc agagaaaaaa ggaccaggtt ggactacgat tattcggaaa
    24901 gaggtactga ttaaattatc agtatgcgga attccgagta atccgagtaa cccagatgct
    24961 gatggtgtgt agtgttattc taaggtttaa gcaaagttat gcggaactaa gctttggtat
    25021 atagccataa cgtttggcta acctatggca ataatttacg cactatcagc cacttagagg
    25081 taggaaaaga atgacgaatt taaaagcagt tataccggta gcaggtcttg ggatgcatat
    25141 gttgcctgcc actaaggcga ttcccaaaga gatgctaccg atcgtcgaca agccaatgat
    25201 tcagtacatt gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc tggtaactca
    25261 cgcgtccaag aacgcggtcg aaaaccactt cgacacctct tatgaattag aatctctcct
    25321 tgagcagcgc gtgaagcgtc aactactggc ggaagtgcaa tctatctgcc cgccgggcgt
    25381 gactattatg aacgtgcgtc agggcgaacc tttaggttta ggtcactcca ttttgtgtgc
    25441 acgccccgcc attggtgaca acccatttgt cgtggtgctg ccggacgttg ttatcgatga
    25501 tgccagcgcc gacccgctgc gttacaacct tgccgctatg attgcgcgct tcaacgaaac
    25561 tggccgcagc caggtgctgg caaaacgtat gccgggtgac ctctctgaat actccgtcat
    25621 ccagaccaaa gaaccgctgg atcgtgaagg caaagtcagc cgcattgttg aattcatcga
    25681 aaaaccggat cagccgcaga cgctggactc agacatcatg gccgttggtc gctatgtgct
    25741 ttctgccgat atttggccgg aactggaacg cactcagcct ggtgcatggg ggcgtattca
    25801 gctgactgat gctattgccg aactggcgaa aaaacagtcc gttgatgcca tgctgatgac
    25861 tggtgacagc tacgactgtg gtaaaaaaat gggctatatg caggcgtttg tgaagtatgg
    25921 actgcgcaac ctgaaagaag gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga
    25981 ataatgaaaa tctgaccgga tgtaacggtt gataagaaaa ttataacggc agtgaagatt
    26041 cgtggcgaaa gtaatttgtt gcgaatattc ctgccgttgt tttatataaa caatcagaat
    26101 aacaaagagt tagcaatagg attttcgtca aagttttcca ggattttcct tgtttccaga
    26161 gcggattggt aagacaatta gcgtttgaat ttttcgagtt aagcgcgagt gggtaacgct
    26221 cgtcacatcg taggcatgca tgcagtgctc tggtagctgt aaagccaggg gcggtagcgt
    26281 gcattaatac ctctattaat caaactaaga gccgcttatt tcacagcatg ctctgaagta
    26341 atatggaata aaagagtgaa gatacttgtt actggtggcg caggatttat tggttctgct
    26401 gtagttcgtc acattataaa taatacgcag gatagtgttg ttaatgtcga taaattaacg
    26461 tacgccggaa acctggaatc acttgctgat gtttctgatt ctgaacgcta tatttttgaa
    26521 catgcggata tttgcgatgc tgctgcaatg gcgcggattt ttgctcagca tcagccggat
    26581 gcagtgatgc acctggctgc tgaaagccat gtggatcgtt caattacagg ccctgcggca
    26641 tttattgaaa ccaatattgt tggtacttat gtccttttgg aagccgctcg caattattgg
    26701 tctgctcttg atggcgacaa gaaaaatagc ttccgttttc atcatatttc tactgacgaa
    26761 gtctatggtg atttgcctca tcctgacgaa gtaaataata aagaacaatt acccctcttt
    26821 actgagacga cagcttacgc gcctagtagt ccttattccg catcaaaagc atccagcgat
    26881 catttagtcc gtgcgtggaa acgtacctat ggtttaccga ccattgtgac taattgttcg
    26941 aataactacg gtccttatca ctttccggaa aaattgattc cactagtaat tcttaatgct
    27001 ctggaaggta aggcattacc tatttatggc aaaggggatc aaattcgcga ctggctgtat
    27061 gttgaagatc atgcacgtgc gttatatacc gtcgtaaccg aaggtaaagc gggtgaaact
    27121 tataacattg gtggacacaa cgaaaagaaa aacatcgatg tagtgctcac tatttgtgat
    27181 ttgttggatg agattgtacc gaaagagaaa tcttaccgcg agcaaattac ttatgttgcc
    27241 gatcgtccgg gacacgatcg ccgttatgcc attgatgctg agaagattgg tcgcgaattg
    27301 ggatggaaac cacaggaaac gtttgagagc gggattcgga agacagtgga atggtacctg
    27361 tccaatacaa aatgggttga taatgtgaaa agtggtgcct atcaatcgtg gattgaacag
    27421 aactatgagg gccgccagta atgaatattc tccttttcgg caaaacaggg caggtaggtt
    27481 gggaactaca gcgtgctctg gcacctttgg gtaatttgat tgctcttgat gttcactcca
    27541 ctgattattg tggtgatttt agtaatcctg aaggtgtggc tgaaaccgtc aaaaaaattc
    27601 gccctgatgt tattgttaat gctgcggctc ataccgcagt agataaggct gagtcagaac
    27661 ccgaatttgc acaattactc aatgcgacca gcgttgaatc aattgcaaaa gcggctaatg
    27721 aagttggggc ctgggtaatt cattactcaa ctgactacgt atttccggga accggtgaaa
    27781 taccatggct ggagacggat gcaaccgcac cgctaaatgt ttacggtgaa accaagttag
    27841 ctggagaaaa agcattacaa gagcagtgtg cgaagcacct tattttccgt accagctggg
    27901 tatatgcagg taaaggaaac aattttgcca agacaatgtt acgtctggca aaagagcgtg
    27961 aagaattagc cgttattaat gatcagtttg gtgcgccaac tggcgcagag ttgctggctg
    28021 attgtacggc acatgccatt cgtgtggcac tgaataaacc ggaagtcgca ggtttgtacc
    28081 atctggtagc cagtggtacc acaacctggc acgattatgc tgcgctggtt tttgaagagg
    28141 cgcgcaaagc aggcataccc cttgcactca acaagctcaa cgcagtagca acaacagcct
    28201 atcctacacc agctcgtcgt ccacataact ctcgccttaa tacagaaaaa tttcagcaga
    28261 attttgcgct tgttttgcct gactggcagg ttggcgtgaa acgaatgctc aacgaattat
    28321 ttacgactac agtaatttaa tagtttttgc atcttgttcg tgatgatgga gcaagatgaa
    28381 ttaaaaggaa tgatgaaatg aaaacgcgta aaggtattat tttagcgggt ggctctggta
    28441 ctcgtcttta tcctgtgact atggctgtca gtaaacagct gttacctatt tatgataaac
    28501 cgatgatcta ttacccgctt tctacactga tgttagcggg tattcgcgat attctgatta
    28561 tcagtacacc acaggatact cctcgttttc accaactgct gggtgacggg ggccagtggg
    28621 ggctaaatct tcagtacaaa gtgcaaccga ctccagatgg gcttgcgcag gcgtttatta
    28681 tcggtgaaga gtttatcggt ggtgatgatt gtgctctggt tctcggtgat aatatctttt
    28741 acggtcatga tctgccgaag ttaatggatg tcgctgttaa caaagaaagt ggtgcaacag
    28801 tatttgccta tcatgttaat gatcctgaac gctacggtgt cgttgagttt gataaaaaag
    28861 gtacggcaat tagcctggaa gaaaaaccgt tacaaccaaa aagtaattat gcggtaaccg
    28921 ggctttattt ctatgataac gacgttgtcg aaatggcgaa aaaccttaag ccttctgccc
    28981 gtggtgaact ggaaattacc gatattaacc gtatttatat ggaacagggg gggttatccg
    29041 ttgccatgat gggacgtggt tatgcatggc tggacacggg tacacatcaa agtcttattg
    29101 aagcaagcaa cttcattgca acaattgaag agcgtcaggg gttaaaagtt tcctgtccgg
    29161 aagaaattgc ttaccgtaaa gggtttattg atgctgagca ggtgaaagta ttagctgaac
    29221 cgctgaaaaa aaatgcttat ggtcagtatc tgctgagaat gattaaaggt tat
//
LOCUS       DAZKGF010000045        29136 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZKGF010000045 DAZKGF010000000
VERSION     DAZKGF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.045"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            83..1036
                     /gene="ybhD"
                     /locus_tag="ACHOQV_001900"
     CDS             83..1036
                     /gene="ybhD"
                     /locus_tag="ACHOQV_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9630284.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1077..2072)
                     /gene="pgl"
                     /locus_tag="ACHOQV_001901"
     CDS             complement(1077..2072)
                     /gene="pgl"
                     /locus_tag="ACHOQV_001901"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFP9630285.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSEDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2227..3045
                     /gene="ybhA"
                     /locus_tag="ACHOQV_001902"
     CDS             2227..3045
                     /gene="ybhA"
                     /locus_tag="ACHOQV_001902"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFP9630286.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3046..4104)
                     /gene="modC"
                     /locus_tag="ACHOQV_001903"
     CDS             complement(3046..4104)
                     /gene="modC"
                     /locus_tag="ACHOQV_001903"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFP9630287.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4107..4796)
                     /gene="modB"
                     /locus_tag="ACHOQV_001904"
     CDS             complement(4107..4796)
                     /gene="modB"
                     /locus_tag="ACHOQV_001904"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFP9630288.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4796..5569)
                     /gene="modA"
                     /locus_tag="ACHOQV_001905"
     CDS             complement(4796..5569)
                     /gene="modA"
                     /locus_tag="ACHOQV_001905"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9630289.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5735..5884)
                     /gene="acrZ"
                     /locus_tag="ACHOQV_001906"
     CDS             complement(5735..5884)
                     /gene="acrZ"
                     /locus_tag="ACHOQV_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFP9630290.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            6013..6801
                     /gene="modE"
                     /locus_tag="ACHOQV_001907"
     CDS             6013..6801
                     /gene="modE"
                     /locus_tag="ACHOQV_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFP9630291.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARNHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6869..8341
                     /gene="modF"
                     /locus_tag="ACHOQV_001908"
     CDS             6869..8341
                     /gene="modF"
                     /locus_tag="ACHOQV_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFP9630292.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARAQAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            8602..9618
                     /gene="galE"
                     /locus_tag="ACHOQV_001909"
     CDS             8602..9618
                     /gene="galE"
                     /locus_tag="ACHOQV_001909"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP9630293.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9628..10674
                     /gene="galT"
                     /locus_tag="ACHOQV_001910"
     CDS             9628..10674
                     /gene="galT"
                     /locus_tag="ACHOQV_001910"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFP9630294.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10678..11826
                     /gene="galK"
                     /locus_tag="ACHOQV_001911"
     CDS             10678..11826
                     /gene="galK"
                     /locus_tag="ACHOQV_001911"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP9630295.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDATIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11820..12860
                     /gene="galM"
                     /locus_tag="ACHOQV_001912"
     CDS             11820..12860
                     /gene="galM"
                     /locus_tag="ACHOQV_001912"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFP9630296.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPF
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYTDWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            13063..13815
                     /gene="gpmA"
                     /locus_tag="ACHOQV_001913"
     CDS             13063..13815
                     /gene="gpmA"
                     /locus_tag="ACHOQV_001913"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9630297.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13963..15015)
                     /gene="aroG"
                     /locus_tag="ACHOQV_001914"
     CDS             complement(13963..15015)
                     /gene="aroG"
                     /locus_tag="ACHOQV_001914"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFP9630298.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            15331..15711
                     /gene="ybgS"
                     /locus_tag="ACHOQV_001915"
     CDS             15331..15711
                     /gene="ybgS"
                     /locus_tag="ACHOQV_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFP9630299.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15825..16766
                     /gene="zitB"
                     /locus_tag="ACHOQV_001916"
     CDS             15825..16766
                     /gene="zitB"
                     /locus_tag="ACHOQV_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFP9630300.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16763..17482)
                     /gene="pnuC"
                     /locus_tag="ACHOQV_001917"
     CDS             complement(16763..17482)
                     /gene="pnuC"
                     /locus_tag="ACHOQV_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP9630301.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQMFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17520..18563)
                     /gene="nadA"
                     /locus_tag="ACHOQV_001918"
     CDS             complement(17520..18563)
                     /gene="nadA"
                     /locus_tag="ACHOQV_001918"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFP9630302.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCSVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPV
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18811..18886)
                     /locus_tag="ACHOQV_001919"
     tRNA            complement(18811..18886)
                     /locus_tag="ACHOQV_001919"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18851..18853),aa:Lys,seq:ttt)
     gene            complement(18920..18995)
                     /locus_tag="ACHOQV_001920"
     tRNA            complement(18920..18995)
                     /locus_tag="ACHOQV_001920"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18960..18962),aa:Lys,seq:ttt)
     gene            complement(19142..19217)
                     /locus_tag="ACHOQV_001921"
     tRNA            complement(19142..19217)
                     /locus_tag="ACHOQV_001921"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19182..19184),aa:Lys,seq:ttt)
     gene            complement(19221..19296)
                     /locus_tag="ACHOQV_001922"
     tRNA            complement(19221..19296)
                     /locus_tag="ACHOQV_001922"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19261..19263),aa:Val,seq:tac)
     gene            complement(19348..19423)
                     /locus_tag="ACHOQV_001923"
     tRNA            complement(19348..19423)
                     /locus_tag="ACHOQV_001923"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19388..19390),aa:Lys,seq:ttt)
     gene            complement(19426..19501)
                     /locus_tag="ACHOQV_001924"
     tRNA            complement(19426..19501)
                     /locus_tag="ACHOQV_001924"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19466..19468),aa:Val,seq:tac)
     gene            complement(19637..19712)
                     /locus_tag="ACHOQV_001925"
     tRNA            complement(19637..19712)
                     /locus_tag="ACHOQV_001925"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19677..19679),aa:Lys,seq:ttt)
     gene            complement(19877..20668)
                     /gene="cpoB"
                     /locus_tag="ACHOQV_001926"
     CDS             complement(19877..20668)
                     /gene="cpoB"
                     /locus_tag="ACHOQV_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFP9630303.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     EAIVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20678..21199)
                     /gene="pal"
                     /locus_tag="ACHOQV_001927"
     CDS             complement(20678..21199)
                     /gene="pal"
                     /locus_tag="ACHOQV_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFP9630304.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21234..22526)
                     /gene="tolB"
                     /locus_tag="ACHOQV_001928"
     CDS             complement(21234..22526)
                     /gene="tolB"
                     /locus_tag="ACHOQV_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFP9630305.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVFDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22659..23924)
                     /gene="tolA"
                     /locus_tag="ACHOQV_001929"
     CDS             complement(22659..23924)
                     /gene="tolA"
                     /locus_tag="ACHOQV_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFP9630306.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(23989..24417)
                     /gene="tolR"
                     /locus_tag="ACHOQV_001930"
     CDS             complement(23989..24417)
                     /gene="tolR"
                     /locus_tag="ACHOQV_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFP9630307.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24421..25113)
                     /gene="tolQ"
                     /locus_tag="ACHOQV_001931"
     CDS             complement(24421..25113)
                     /gene="tolQ"
                     /locus_tag="ACHOQV_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFP9630308.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(25110..25514)
                     /gene="ybgC"
                     /locus_tag="ACHOQV_001932"
     CDS             complement(25110..25514)
                     /gene="ybgC"
                     /locus_tag="ACHOQV_001932"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP9630309.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25664..25957)
                     /gene="ybgE"
                     /locus_tag="ACHOQV_001933"
     CDS             complement(25664..25957)
                     /gene="ybgE"
                     /locus_tag="ACHOQV_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFP9630310.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(25957..26070)
                     /gene="cydX"
                     /locus_tag="ACHOQV_001934"
     CDS             complement(25957..26070)
                     /gene="cydX"
                     /locus_tag="ACHOQV_001934"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFP9630311.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(26085..27224)
                     /gene="cydB"
                     /locus_tag="ACHOQV_001935"
     CDS             complement(26085..27224)
                     /gene="cydB"
                     /locus_tag="ACHOQV_001935"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP9630312.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFHLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(27240..28808)
                     /gene="cydA"
                     /locus_tag="ACHOQV_001936"
     CDS             complement(27240..28808)
                     /gene="cydA"
                     /locus_tag="ACHOQV_001936"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFP9630313.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7264 a         7733 c         7154 g         6985 t
ORIGIN      
        1 attaatttaa gttggctaaa tgcagttaaa atttttaact gccagtcacc caaaatcgtc
       61 gcttccattt tccggagtaa taatgaagca tgaattatca agtatgaagg catttgtcat
      121 actggcagag tccagttcat ttaacaatgc tgctaaatta ctcaatatta cgcaacctgc
      181 attaacgcga agaataaaaa agatggagga agatttacac attcaactat ttgagcgtac
      241 aactcgcaag gttacgttaa caaaagcagg aaaaaggttg ctcccggagg cgcgggaatt
      301 aataaaaaaa ttcgatgaaa cgctttttaa tattcgtgat atgaatgctt atcatcgtgg
      361 tatggtgacg ttagcgtgta ttccgaccgc agtattttat tttttaccgc tggcaatcgg
      421 taaatttaat gagctgtatc ccaatattaa agtgcggatt ctggaacaag gcacgaataa
      481 ttgcatggaa tcggtgctgt gtaacgaatc agactttggc ataaacatga acaacgtcac
      541 aaattcatcc attgatttta ccccactggt caacgagccg ttcgtgctgg cctgtcggcg
      601 tgaccatcca ttggccaaaa agcagcttgt agaatggcag gagttagtgg gttacaaaat
      661 gattggcgta cgctcctcca gcggcaaccg actattgata gaacaacagc tagccgacaa
      721 gccctggaag ctggactggt tttacgaagt gcgtcatctt tcgacgtcat tagggctggt
      781 ggaggcggga ctggggattt cggcgcttcc tggtctggca atgccccatg cgccctattc
      841 ttcgattatt ggtatcccgc tggtggaacc ggttatacgt cggacattgg ggattattcg
      901 ccgcaaagat gccgtgcttt ctccggcagc tgaacgcttt tttgccttgc tgattaattt
      961 gtggactgac gataaagaca atttgtggac caatatcgtc gaacgccaga gacatgcgtt
     1021 acaggagata ggttaataca ccggcgcaga agagactgcg ccgggtaaat cagcggttag
     1081 tgtgcgttaa ccaccaccca cattggtccc tgcccgaccg catagcggcc tttttcatgc
     1141 agtagcccct gctcgccaac aatttcgtat accgagatgt ggtgagattt ttgcccggcg
     1201 gcaatcagat acttgccgct gtgatcaaca ttgaagccgc gcggctgggt ttcagttggc
     1261 tggaagcctt ctttactcaa cacgctgcca tcttccgaaa cgctgaaaac agtaatcaga
     1321 ctggcggtac ggtcgcaggc gtataaatga cgaccatccg gggtgatatg aatatcagcc
     1381 gcccaacggg tgtcggagaa gttttccggc atcatatcca gcgtctggac acattcgata
     1441 ttgccatgcg gatctttcag ttcccagaca tccactgagc tgtttaactc attgacgcaa
     1501 tacgcatatt gttcgtttgg atggaatacc atatgacgcg ggccggcccc ttcaacggta
     1561 gtcacttccg cagggtcctg cgccaccaga tgaccatcct cgctgacagt aaacaggcaa
     1621 atgcgatcct gctttaatgc cggaacccac agcgtacggt tgtccggtga gatattggcg
     1681 gaatggcaac cgtccagccc ctcgaccaca tcgacgacgc ccactggcag gccatcttcc
     1741 agacgcgtta cgctcacgtt acccgcattg taagaaccga caaagacaaa ctgcccctgg
     1801 tgatcggtgg aaatatgcgt cggactaccc ggcagcgcag actctgcggc aaaggtcagc
     1861 gcgccatcgt ccggggcgat acgatacgcc aggacgcgaa actcagggcg aacaccaaca
     1921 tagagataac gtttgtccgg gctgaccacc atcggctgca cctgccccgg cacgtcgaca
     1981 acctgtgtca gcgtcagtgc gccttcatga ttcaggttcc agacgtgaat ttgctggctc
     2041 tcagggctgg cgatataaac tgtttgcttc atgaatgctc ctttgcattt agcaactgac
     2101 tgtaacagct aaaattagtc gcttttggcg gtgaatgctg aattaaaacg cagaattttg
     2161 tccgatagtg gtatcggtgt accattcgcg cagacgaaaa ttcttaacct taatctggaa
     2221 tacgccatga ccacacgcgt gattgctctc gacttagacg gcaccttatt gaccccgaaa
     2281 aagaccttgc ttccatcatc gatagaagcc ctggcccgcg ctcgcgaagc aggctatcaa
     2341 ttaatcatcg tcacaggtcg ccatcacgtc gctattcatc ctttttatca ggcgctggcg
     2401 ctggatacac ctgctatttg ctgtaatggc acctatttgt atgattatca tgcaaaaaca
     2461 gtgctggaag cggacccaat gcccgttaat aaagccctgc aactcattga gatgctgaat
     2521 gaacaccaca ttcacggtct gatgtatgtc gatgatgcaa tggtctatga gcacccgacc
     2581 ggacatgtca ttcgcacgtc taactgggcg caaaccctgc cgccggaaca gcgtccgact
     2641 ttcacacaag tcgcttctct ggctgaaacg gcgcaacaag ttaacgccgt atggaagttc
     2701 gccctgacgc acgatgacct gccgcaattg cagcattttg gtaagcatgt cgaacatgaa
     2761 ctgggactgg agtgtgaatg gtcctggcac gatcaggttg atattgcacg cggcggcaac
     2821 agcaaaggta aacgtttgac gaaatgggtt gaggcgcaag gctggtcgat ggaaaacgtc
     2881 gtggccttcg gcgataactt caatgatatt agtatgctgg aagccgctgg tacaggcgtg
     2941 gcgatgggca acgccgatga cgcagtaaaa gcgcgcgcca acattgtgat tggtgataac
     3001 accaccgaca gcattgccca gttcatttat agccacctga tttaatcagg cggttatcga
     3061 cacactttta atttgcgcat acagccacag gccaggtttg atcgccagtt catccctggc
     3121 ccacgggctg atacgcgccc acagcgtttt accgccgact tccagctcca cttccacctg
     3181 gccgttgtcg tcataactat taaccacttt tgcccgcaat acgttacgaa tgctggtttg
     3241 ctgcggcggt tgtaaaacca gcgagacatc ggaagcctga atgcggatgc gtagcgcagc
     3301 ttgcagcggt tcgtccagct tattgaccca caaatgctga tcgcccagcg ccagcgcggt
     3361 catcgcgtaa tgcggatgat gctcaagcac cgtcactttc agaatgctac tttgttgctc
     3421 tttcggcagc cacggattca tcacgctact gccccacact tcctccagcg cgccaaaggc
     3481 tttcacctga ccgttttcca gtaccatcac tctgtctgcc agatggagga tctcatccag
     3541 cgaatggctg acatacaaca tcggaatgtt gatttcccgt gtcagccgtt gcagataagg
     3601 caacagttcg cgtttacgcg gaatatccag tgacgccagc ggttcatcca gcaacagcaa
     3661 ttccggtgct gtcagcaaag cccgaccaat cgccacgcgc tgtttttcac ctccggacag
     3721 gctgcctggt aaacggtcaa gcaacggttc aatgcctaaa agcgccacca gcttatcgaa
     3781 ctgatcgacc atacttttcg ccatgccgta gcgcagattg ccacgcactt tgtaatgcgg
     3841 gaacagccgc gcatcctgaa aaacatagcc aacgcgacgc ttttccggcg tcaggcagat
     3901 acctttttcg gcatcattta gtacccgccc attgaggaca atccgccctt tttgcgggcg
     3961 cgtcagtcca ctgatggcgt taatcagcga agtttttccg gcaccggaga cgccaaagat
     4021 agcagtgatg ccattggcgg gcagcgtttc attaatggtc aggcaatggt tgcccaacgt
     4081 ctgggaaaaa ttcagttcca gcatgattag cgccccgccc gttcacggct gattctggcc
     4141 agccattctg aaatcaacag ggagatcatc gccagtgcaa tagaaataat gcacagtctc
     4201 gccgctccac tttccccgcc gggggtctgg atcagggtat acatggcaga aggaatggtt
     4261 cgcgtttcac caggaatgtt cgacacaaag gtgatggttg caccaaactc accgagagaa
     4321 cgagcaaaag ccagtaccgt accaacaata atgcccggta aggtcagcgg taacgtgata
     4381 gtaaagaaaa cgcgccagcg cccggcaccc agtgttcttg cggcctgttc cagtttgaca
     4441 tcaacccctt ccagcgccag acgaattgcc cgcaccatca gcggaaacga catgacggcg
     4501 gcagcgagaa ccgcgccgcg ccagctaaag gcgaaggtaa tgccaaacca gtcatacaga
     4561 cgttcaccga taaatccgcg ccgtcccatc gaaactaata ataagtaacc gacgaccacg
     4621 ggcggtaaca ccagcggtag atgcagtacg ctgtcgagca gagctttgcc cggaaacgtg
     4681 caacgcacca gtaaccaggc aaaaaagatc ccaaacggca ggctaaacag cacagccagg
     4741 gaagaaactt tcaggcttag taaaaccgcc tgccattctg gatcggtcag tatcattact
     4801 tggttgtaaa tccgtaacgt ttaaagattt ccgcagcctg cggtcccttc agataatcat
     4861 aaaatgcttt caccgtcgca ttgttatgcc cttccacaac agcaaccgga tattccactt
     4921 ttttatgcga atcttccggg aaggtggcaa ccacttttac ccctttgctg gcaactgcgt
     4981 cagaaccgta aacaataccc agcggcgctt cattgcgttc gaccaacgcc agcgcgccac
     5041 gaacatcttc cgccggagcc agtttcgggg agagcgtatc ccatgcgccc agtttttgca
     5101 gtgcttcttt tgcataaatg ccagcgggaa catgttccgg atcgccaacc gccagacgac
     5161 cgccattcag cagcgaagtc cagttggttt tgctgtcgat ggtgaaatct ttctgctcgc
     5221 tggctttcgg tgctacaacg accaggctat tgccgagcag tgtctggcgc gaagccgtat
     5281 cgatcgcttt tttatcaacc gcataatcca tccatttctg atcggcagaa ataaacagat
     5341 ccgcaggcgc acccgcttca atctgacggg ccaaagtaga tgacgaagcg aaagaagaaa
     5401 ccacatccac gcctttctct tttttatact gcgtagcaat gtcctgcatt gcgttagtca
     5461 gtgatgcggc ggcgaacacc gtgattttcc cttcatctgc cagtgcattg ccagcaacag
     5521 cgaaagagag tgccgccccg gcaaacaagt tcaaccattt acgagccatt ggtaacccct
     5581 taatctaacg ttatgtaggc gacaatataa cggctaactc agaattttcc cagcagttat
     5641 tgctaacctt tgtgaggtag atgagaaaaa atatcggcag gaaaagcagg aggttgagaa
     5701 aaagaaatgc ccgactaagc gggcattcag ggattcaatg attttgtccg ggctggtctt
     5761 ttttaccaac accagaaaag atgttgaata cttcaccaag accgtaaatc agacccagga
     5821 tgatggccat cacgacaggt accatgatta cggcgaatac cagacttttt aataactcta
     5881 acatggtcaa ctccagatat agtcatgaga ctattctaac cgctaagcaa agaaaagcac
     5941 tccccttttg tgcggtcagc tttgcgtggc gttcgttttc cgtcacaata agactttttc
     6001 caggatattg ttatgcaggc cgaaatcctt ctcaccctta agctccaaca aaaattattc
     6061 gccgacccgc gccgcatttc gctactaaaa cacattgcgc tttccggttc cattagccag
     6121 ggagcgaaag atgccggtat cagctataaa agcgcatggg atgccattaa cgagatgaat
     6181 cagttaagtg agcatattct ggttgagcgc gcaacaggcg gtaaaggtgg aggcggcgca
     6241 gtactgaccc gctatggtca gcgactgatt cagctctatg acttactggc gcaaatccag
     6301 caaaaagcct tcgatgtgtt aagtgacgat gacgccctgc cgctgaacag cctgctggcc
     6361 gcgatctcac gtttttcact gcaaaccagc gcccgtaacc aatggtttgg caccatcacc
     6421 gcccgcaatc atgatgacgt tcaacagcat gttgatgtct tactggctga cggaaaaaca
     6481 cgcctgaaag tcgcaattac cgcacaaagc ggcgcgcgtc tggggctgga tgaaggcaaa
     6541 gaagtgttga tattactaaa agcgccgtgg gtaggcatta ctcaggacga agcggtcgca
     6601 caaaacgccg acaaccaatt accgggtatt attagccata ttgagcgcgg cgcagagcag
     6661 tgcgaagtat taatggcgct acccgacggg caaacactgt gcgccacagt gccggtaaat
     6721 gaagcgactt ctcttcagca aggacagaat gtcacggcct actttaatgc cgacagcgtg
     6781 attatcgcca cgctgtgcta agcgtgttga caatttgtta tgaaacacgt atccctgtca
     6841 gtaatcgctg cacaaaatgg gatataaaat gtcatcgttg caaattttgc aaggcacgtt
     6901 tcgtcttagc gacacaaaaa cgctgcaatt gcctcagcta acgttaaacg cgggtgatag
     6961 ttgggcgttt gtcggttcga atggaagcgg gaaatcggcg ctggcccgcg cgcaggcggg
     7021 ggaacttccg cttttgaaag gtgaacggca aagccagttt tcccacatca ctcgtctctc
     7081 cttcgagcaa ttgcaaaagc tcgtcagcga cgaatggcag cggaataaca ccgatatgct
     7141 cggccctggc gaagatgaca ccgggcgtac cacggctgaa atcattcagg atgaagtaaa
     7201 ggatgcaccg cgttgcatgc aactggcgca gcagttcggt attaccgccc tcctcgaccg
     7261 acgctttaaa tacctttcca ctggcgagac gcgaaaaacc ctgctgtgtc aggcgctgat
     7321 gtcggagcct gacttgttga ttcttgatga gccgttcgat ggcctggatg ttgcctcacg
     7381 tcagcagctg gctgagctac tcgcctcgtt acatcagtcc ggtattactc tggtactggt
     7441 gctcaatcgc ttcgatgaga tcccggaatt tgtccagttt gctggcgtgc tggcggattg
     7501 tacgttagcg gaaacgggcg ctaaagagga actgctccag caagcactcg tcgcgcaact
     7561 ggcacatagc gaacagcttg aaggtgtgca actgccggag ccggatgaac cttcagcacg
     7621 tcacgcctta cccgccaacg aaccgcgcat tgtgctgaac aacggcgtgg tttcttataa
     7681 cgatcgcccc attcttaata accttagctg gcaggtgaat ccaggcgagc actggcaaat
     7741 tgtcgggcca aatggcgcgg gaaaatcgac gttattaagc ctgattactg gcgatcatcc
     7801 gcaaggttac agcaacgatt tgacgctttt cggacgacgt cgcggcagcg gcgaaaccat
     7861 ctgggatatc aaaaagcata tcggttacgt cagcagtagt ttgcatctgg attaccgggt
     7921 cagcactacc gtgcgtaatg tgatcctttc tggctatttt gattcgattg gcatttatca
     7981 ggccgtttcg gatcgccagc aaaaactggt gcagcagtgg ctggatattc tcggcattga
     8041 taaacgcacg gctgacgctc cgttccatag tctttcctgg ggacagcagc gtctggcgct
     8101 gatcgtccgc gcgctggtga aacatccgac gttgcttatt ctcgatgaac cactacaggg
     8161 gcttgatccg ctcaatcgcc agcttatccg ccggtttgtt gatgtgctga ttagcgaagg
     8221 tgaaacgcaa ttgttgtttg tttcgcacca tgctgaagat gcgcctgcct gtattaccca
     8281 tcgtcttgag ttcgtgccgg acggtgactt ttatcgctat gcgctgacaa aaataaactg
     8341 agtcggtaat gctgactgta ccggaggcgg cctaagcgcc ctctccggcc tacagttcga
     8401 cgcatccagg catgaaaccg cgactttttt cagataaaaa gctcaatcat tcataaaccc
     8461 tctgttttat aatccattaa tggcgcataa aaaacggcta aattcttgtg taaacgattc
     8521 cactaattta ttccatgtca cacttttcgc atctttgtta tgctatggtt atttcatatc
     8581 ataagcctaa tggagcgaat tatgagagtt ctggttaccg gtggtagcgg ttacattgga
     8641 agtcatacct gtgtgcaatt actgcaaaac ggtcatgatg tcatcattct tgataacctc
     8701 tgtaacagta agcgcagcgt actgcctgtt atcgagcgtt taggcggcaa acatccaacg
     8761 tttgttgaag gcgatatccg taacgaagcg ttgatgaccg agatcctgca cgatcacgct
     8821 atcgacactg tgatccactt cgccgggctg aaagccgttg gcgaatcggt acaaaaaccg
     8881 cttgaatatt acgacaacaa tgtcaacggt accctgcgcc tgattagcgc catgcgcgcc
     8941 gcgaacgtca aaaactttat ttttagctcc tccgccaccg tctatggcga tcagcccaaa
     9001 attccatacg ttgaaagctt cccgaccggc acaccgcaaa gcccttacgg caaaagcaaa
     9061 ctgatggtgg aacagatcct caccgatctg caaaaagccc agccggactg gagcattgcc
     9121 ctgctgcgct acttcaaccc ggttggcgcg catccgtcgg gcgatatggg cgaagatccg
     9181 caaggcattc cgaataacct gatgccatac atcgcccagg ttgctgtagg ccgtcgcgac
     9241 tcgctggcga tttttggtaa cgattatccg accgaagacg gtactggcgt acgcgattac
     9301 atccacgtaa tggatctggc ggacggtcac gtcgtggcga tggaaaaact ggcgaacaag
     9361 ccaggcgtac acatctataa cctcggcgct ggcgtaggca gcagcgtgct ggacgtggtt
     9421 aatgccttca gcaaagcctg cggcaaaccg gttaattatc attttgcacc gcgtcgcgag
     9481 ggcgaccttc cggcctactg ggcggacgcc agcaaagccg accgtgaact gaactggcgc
     9541 gtaacgcgca cactcgatga aatggcgcag gacacctggc actggcagtc acgccatcca
     9601 cagggatatc ccgattaagg aacgaccatg acgcaattta atcccgttga tcatccacat
     9661 cgccgctaca acccgctcac cgggcaatgg attctggttt caccgcaccg cgctaagcgc
     9721 ccctggcagg gggcgcagga aacgccagcc aaacaggtgt tacctgctca tgatccagat
     9781 tgcttcctct gcgcaggtaa tgtgcgggtg acaggcgata aaaaccccga ttacaccggg
     9841 acttacgttt tcactaatga ctttgcggct ttgatgtctg acacaccaga tgcgccggaa
     9901 agtcacgatc cgctgatgcg ttgccagagc gcgcgtggca ccagtcgggt gatctgcttt
     9961 tcaccggatc acagcaaaac gttgccagaa ctcagcgttg cggcattgac ggaaatcgtc
    10021 aaaacctggc aggagcaaac cgcagaactg gggaaaacgt acccatgggt gcaggtcttt
    10081 gaaaacaaag gcgcggcgat gggctgctct aacccgcatc cgcacggaca gatttgggca
    10141 aatagcttcc tgcctaacga agctgagcgc gaagaccgtc tgcaaaaaga atatttcgcc
    10201 gaacagaaat cgccaatgct ggtggattat gttcagcgcg agctggcaga cggtagccgt
    10261 accgttgtcg aaaccgaaca ctggttagcc gttgtacctt actgggctgc ctggccgttc
    10321 gaaacgctac tgctgcccaa agcccacgtt ttacggatca ccgatttgac cgacgcccag
    10381 cgcagcgatt tggcactggc gttgaaaaag ctgaccagtc gttatgacaa cctcttccag
    10441 tgctccttcc cctactctat gggctggcac ggcgcgccat ttaatggcga agagaatcaa
    10501 cactggcagc tgcacgcgca cttttatccg cctctgttgc gctctgccac cgtacgtaaa
    10561 tttatggttg gttatgaaat gctggcagaa acccagcgag acctgaccgc agaacaggcc
    10621 gcagagcgtt tgcgcgcagt cagcgatatc cattttcgcg aatccggagt gtaagaaatg
    10681 agtctgaaag aaaaaacaca atctctgttt gccaacgcat ttggctaccc tgccactcac
    10741 accattcagg cgcctggccg cgtgaatttg attggtgaac acaccgacta caacgacggt
    10801 ttcgttctgc cctgcgcgat tgattatcaa accgtgatca gttgtgcacc acgcgatgac
    10861 cgtaaagttc gcgtgatggc agccgattat gaaaatcagc tcgacgagtt ttccctcgat
    10921 gcgaccattg tcgcgcatga aaactatcaa tgggctaact acgttcgtgg cgtggtgaaa
    10981 catctgcaac tgcgtaacaa cagcttcggc ggcgtggaca tggtgatcag cggcaatgtg
    11041 ccgcagggtg ccgggttaag ttcttccgct tcactggaag tcgcggtcgg aaccgtattg
    11101 cagcagcttt atcatttgcc gctggacggc gcacaaatcg cgcttaacgg tcaggaagca
    11161 gaaaaccagt ttgttggctg taactgcggg atcatggatc agctaatttc cgcactcggc
    11221 aagaaagatc atgccttgct gattgactgc cgctcactgg ggactaaagc agtttccatg
    11281 ccgaaaggtg tggctgtcgt catcattaac agtaacttca aacgtaccct ggttggcagc
    11341 gaatacaaca cccgtcgtga acagtgcgaa actggtgcgc gtttcttcca gcagccagcg
    11401 ctgcgcgatg tcaccattga agagttcaac gctgttgcgc atgaactgga cccaatcgtg
    11461 gcgaaacgcg tgcgtcatat cctgactgaa aacgcccgca ccgttgaagc tgccagcgca
    11521 ctggagcaag gcgacctgaa acgtatgggc gagttgatgg cggagtctca tgcctctatg
    11581 cgcgatgatt tcgaaatcac cgtgccgcaa attgacactc tggtagaaat cgtcaaagct
    11641 gtgattggcg acaaaggtgg cgtacgcatg accggcggcg gatttggcgg ctgtatcgtc
    11701 gcgctgatcc cggaagagct ggtgcctgcc gtacagcaag ctgtcgctga acaatatgaa
    11761 gcaaaaacag gtattaaaga gactttttac gtttgtaaac catcacaagg agcaggacag
    11821 tgctgaacga aactcccgca ctggcacccg atggtcagcc gtaccgactg ttaactttgc
    11881 gtaacaacgc agggatggta gtcacgctga tggactgggg tgcgacttta ctttccgccc
    11941 gtattccgtt ttccgatggc agcgtccgcg aggcgctgct cggctgtgcc agcccggaat
    12001 gctatcagga tcaggccgca tttctggggg cctctattgg tcgttatgcc aaccgtatcg
    12061 ccaatagccg ttataccttt gacggtgaaa ccgtgacgct ttcaccaagt cagggcgtta
    12121 accagctgca cggcggcccg gaagggttcg acaaacgtcg ctggcagatt gtgaaccaga
    12181 acgatcgtca ggtgctgttt gccctgagtt cagatgatgg cgatcagggc ttcccgggta
    12241 atctcggagc gacggtacaa tatcgtctga ccgacgataa ccgtatctct attacttatc
    12301 gcgccacagt tgataaacct tgcccggtga atatgactaa tcacgtctat ttcaatcttg
    12361 acggcgagca gtctgacgtg cgcaatcaca agttgcagat tctggcggac gaatatctgc
    12421 cggttgatga aggcggcatt ccccacgacg gcctgaaatc tgtcgccgga acgtcttttg
    12481 atttccgcag cgccaaaatc atcgccagtg agtttcttgc cgacgacgat cagcgcaaag
    12541 tgaaaggtta cgatcacgcg ttcttgttac aggccaaagg cgatggcaag aaagtggcgg
    12601 cgcatgtctg gtcagcagat gaaaaattgc agctgaaggt ctacaccacc gctccggctc
    12661 tgcaattcta ctccggcaac tacctcggcg gcacaccgtc gcggggaacc gaaccttaca
    12721 ccgactggca agggctggcg ttggaaagcg agtttctgcc ggacagcccg aaccaccctg
    12781 aatggccgca accggactgt ttcctgcgtc ctggcgaaga gtattccagc ctgacggaat
    12841 atcagtttat tgctcagtaa tgttaagccc tcgtcatgag ggctttatct tcatattgtt
    12901 caaatcacca gcaaacaccg acatatttgc aactcaatat tcacaacaac cttacactgc
    12961 gacactattt tcgctatggt tatgcgtaag cattgctgtt gcttcgtcac ggcaatataa
    13021 tgagaattat tatcattaaa agatgatttg aggagtaagt atatggctgt aactaagctg
    13081 gttctggttc gtcatggcga aagtcagtgg aacaaagaaa accgtttcac cggttggtac
    13141 gacgtggatc tgtctgagaa aggcgtaagc gaagcaaaag cagcaggtaa gctgctgaaa
    13201 gaggaaggtt acagctttga ctttgcttac acctctgtgc tgaaacgcgc tatccatacc
    13261 ctgtggaatg tgctggacga actggatcag gcatggctgc ccgttgagaa atcctggaaa
    13321 ctgaacgaac gtcactacgg tgcgttgcag ggtctgaaca aagcagaaac cgctgaaaag
    13381 tatggcgacg agcaggtgaa acagtggcgt cgtggttttg cagtgactcc gccggaactg
    13441 actaaagatg atgagcgtta tccgggtcac gatccacgtt acgcgaagct gagcgagaaa
    13501 gaactgccac tgacggaaag cctggcgctg accattgacc gcgtgatccc ttactggaat
    13561 gaaaccattc tgccgcgtat gaagagcggt gagcgcgtaa tcatcgctgc acacggtaac
    13621 tcgttacgtg cgctggtgaa atatcttgat aacatgagcg aagaagagat tcttgagctg
    13681 aatatcccga ctggtgtgcc actggtgtat gagttcgacg agaatttcaa accgttgaaa
    13741 cgctattatc tgggtaatgc tgacgagatc gcggcgaaag cagcggccgt tgcgaaccag
    13801 ggtaaagcga agtaaacgtc attcgcttaa aaatgagaaa gccgactgca agtgagtcgg
    13861 cttttttgtt tgtcgttgct aacaatggag cacattgcct gatgctacgc tgcgcgtctt
    13921 atcagggctg caaacgtaca gtcatggcga ttcgactata aattacccgc gacgcgcttt
    13981 tactgcattc gccagttgac gtaacagagc atcggtatca tcccagccaa tgcaggcatc
    14041 ggtgatgctc ttaccatagg ccagcggttc cccactctcg aggctctgat tgccttccac
    14101 cagatggctt tccaccatca cgccaataat agccttttcg ccaccggcaa tctgctggca
    14161 aacgtcagca caaacatcca tctgcttttt gaattgtttt gacgagttag catggctgaa
    14221 atcgatcatc acctgtgctg gcaggcctgc tttgttcagc ccttctttca cttcagcaac
    14281 gtgcttcgcg ctgtagttag gctctttacc gccgcgcaga atgatatggc aatcgccgtt
    14341 accgctggta ttcacaatcg ccgaatgccc ccatttcgtt acggacagga agcagtgcgg
    14401 cgcaccggcg gcattaatgg catcgatagc cactttaatc gtaccgtcgg tgccattttt
    14461 gaagccgacc ggacaagaaa gccctgatgc cagttcgcgg tgcacctgcg attcggtggt
    14521 acgtgcgcca attgcgcccc agctcatcag gtcagcgaga tattgtgggg tgatcatatc
    14581 caggaattca cccgccgctg gcagaccgct gtcgttaata tcaagcagca atttacgggc
    14641 aatacgcaga ccgtcgttga tctggaagct gttatccata tgcggatcgt taatcagccc
    14701 tttccagccc accgtggtac gcggcttttc aaaatagacg cgcatcacga tttccagctc
    14761 atcttgcagc tcttcacgca gcgtcagcaa gcgagtggca tactctttcg ccgcgacagg
    14821 atcatgaatt gagcacggac caatcaccac caacaggcga tcatcattac ctttcaggat
    14881 cttatggatc gcttttcggg catgggcgac cgtattcgcg gcattttcag tagcggggaa
    14941 tttttccagc aatgcgactg gaggaagtaa ctctttgatt tctttgatgc gtaaatcgtc
    15001 gttctgataa ttcatgtctg ttccagtgtt gccatactta tcttagtgaa tgcaatactt
    15061 ccaatctata tcttccgtca gaatgtgtaa acggggtttt acactatgaa cggattaatc
    15121 ctggaattag caagaaaatc gccagattgt cgcgaaaaac gagatctctc ctacaatttc
    15181 taactgtaac tcctttgttt gttaattatt tcaagattct ctgctgggta tctttgcctg
    15241 gctgaaatct taaaccattg ccttatattc acctgcaaat gcactgttgg aagaggttat
    15301 ccgacataac gaccataaca ggagcatcct atgaaaatga caaaactggc cacactattt
    15361 ttgactgcca ctctaagcct tgccagcggt gccgcactgg ctgccgatag cggagcgcaa
    15421 agcaataacg gccaggcaaa cgccgcagct gatgcgggcc aggtagcccc tgatgcccgt
    15481 gaaaatgtcg cgccaaataa cgtcgacaat aacggggtaa ataccggttc tggcggcaca
    15541 atgctgcatc cggatggttc ttcaatgaac aatgacggca tgaccaaaga tgaagagcac
    15601 aagaacacca tgtgcaaaga tggtcgctgc ccggatatca ataaaaaagt ccaaaccggt
    15661 gatggcatca acaatgatgt cgataccaaa accgacggta ccacgcagta accctgcgaa
    15721 atagccaata atgggagagc ggagtctctc ccttttaatt tttcccttct aaaagcagta
    15781 tgatgtcata aagttaagtg agaataatta agggatgact cgtcatggcg cactcacact
    15841 cacacacttc ctcacacctg ccagaagata ataatgctcg tcgcttgttg tatgctttcg
    15901 gcgtgactgc cgggtttatg ctcgtagaag tcattggtgg ttttctttcc ggttctctgg
    15961 cattgctggc cgatgcgggt catatgttga ccgatactgc cgccctgctt ttcgccctac
    16021 tcgcagtgca attttcccgt cgtcctccca ccattcgcca cactttcggt tggctaagat
    16081 tgaccacgct cgcggcgttt gtgaatgcca tcgccctggt ggtgattact attttgattg
    16141 tctgggaggc gatagaacgc ttccgcactc cgcgtccggt cgagggcggc atgatgatgg
    16201 caattgccgt ggctgggctg ctggcaaata tactttcttt ctggttactt catcacggca
    16261 gcgaagagaa aaacctcaac gtgcgagcag cggcactgca tgtgctcggg gacctgctgg
    16321 gttcggttgg ggctattatt gccgcgttga ttattatctg gaccggctgg acacctgctg
    16381 accccattct ctcaatactg gtgtcgcttc tggttctgcg tagcgcgtgg cgactgttga
    16441 aagatagcgt gaatgaatta cttgaaggtg caccggtatc gctggatatc gccgaactga
    16501 agcgtcgtat gtgccgggaa atcccggaag tccgcaatgt gcaccatgtg catgtatgga
    16561 tggtaggcga gaagccggtg atgacgctgc atgtgcaggt gatcccgcca cacgatcacg
    16621 atgccttgtt agatcagatc caacactacc tgatggatca ctatcagatt gagcacgcca
    16681 ctattcagat ggaatatcaa ccttgtcatg ggccagactg ccacctgaat gaaggggtgt
    16741 ctggccattc acatcatcac cattaatggg acagcgcgcg tgaacctctt tcgcgtgcgc
    16801 tgttgatcca catccggctg ccgttgaggg caataaaggt caggatgatg tactccagag
    16861 acatggcgta aacgccctga agtgcaaaaa taacgacgct aatcacgtta ataatcaccc
    16921 acaacagcca gttttccaca tacttacgcg tcatcagaat cattgccacg atagataaca
    16981 ccatcatgca tgaatcccag aacgggaaag cgtccggctg tagttcaggc atcgcaacct
    17041 gtaatcctaa tgcttgcatg atcatgactg ccacgcgggt caaaaatgca aacaccggat
    17101 tgataaatac cgtcatcaga ccaatcgaaa caacgcaaac cgccaaccag cttagcgcct
    17161 tcggcaatgg caaccagcga attttcaact ccgcctcgtt ctgactggtt tgtcgcgacc
    17221 acgcatacca gccgtaaata ttcgcggcaa agaaaaacat ctgtaatagc aggctggcat
    17281 acagctgaat ctgaaagaaa ataatgccaa acaaggtgac gttgatcagg ccaaagaagt
    17341 agttgctgat cttctccaga ctggcaaggc caatacacag caacccggcg atcgtgccta
    17401 ccgcttcgat ccatgagaga tcataaccgc ctgcacctat tggtatatgt accaggatat
    17461 tctgcacact aaaaaaatcc atctttcccc caaagcgtaa cgttacgcct tattattcgt
    17521 tatccacgta gtgtagccgc aaaatccagc atacgattga gcggcaccag cgccctctct
    17581 cgcagccttt catcaacatg aacctcgtga ttgcttcctt cctgttctaa tgcctctgcg
    17641 atggcctgaa ggccattcat ggccatccac ggacaatgcg cgcagctgcg gcaggttgca
    17701 ccctcacctg cggttggtgc ttccagtaac tctttaactg gcaccgcctg ctgcattttg
    17761 tagaaaatac cccgatcggt tgccacaata agcctctgat gtggcaatgt tttcgcagca
    17821 gcgatcagtt gactggtgga accgaccgca tccgccatct ctacaatagc ttgcggtgat
    17881 tctggatgca ccagtatggc agcatccggg tattcttctt gcaagcgggt taacgcctga
    17941 gtcttaaact catcatgcac aatgcaggca ccctgccagc ataatatgtc tgcgcccgtc
    18001 tgtttttgca cgtaacgccc cagatgtttg tcgggtgccc agatgatttt ttcacccaaa
    18061 ctatccagat gatcaataag ttcgacggca atgcttgaag ttaccaccca atctgcgcgc
    18121 gcttttaccg cagcagaagt gttggcgtag acgacgacag tacgatcggg atgggcatcg
    18181 caaaatgcgt taaattcttc aacagagcag ccgagatcca gtgaacattc agcctgaagt
    18241 gtcggcatca gaattgtttt ttccggactg agaattttgg cggtttctcc cataaatctc
    18301 accccagcga ctaacaacgt agaagcggga tgctttgcac cgaagcgcgc catttccaga
    18361 gaatcagaaa tacagccacc ggtttcttct gccagttgtt gaatttcggg atcggtatag
    18421 tagtgggcaa ccatcaccgc attacgttct tttagcagac gttttatctt ctcgcggtaa
    18481 tacgcttttt catcaatgct taacggcgtc ggcttcgggg gaaaaggata aatcgccgtg
    18541 tctggatcaa acatcacgct catcttacca tctcgtttta caggcttaac gttaaaaccg
    18601 acatgagcgt aatattcgct gttatcggtg aaatttgttt tatatgctaa acaagatagt
    18661 cgaatggtgg ggaaaagtca ctcgaattac gcctaattcc accgacgtag agttgcttgc
    18721 aaagtaagct ctgaagatgt catcaggtta caaagtgaaa ttgaggatgc ctgaatagca
    18781 aaaaagcgcc tttagggcgc ttttttacat tggtgggtcg tgcaggattc gaacctgcga
    18841 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgaccccttg cgggattatg
    18901 ctgctttaat cattcaggat ggtgggtcgt gcaggattcg aacctgcgac caattgatta
    18961 aaagtcaact gctctaccaa ctgagctaac gacccatacg ggtgttacct gaaagatttc
    19021 tactcggaac caatctttaa aattggtggg tcgtgcagga tgactcgctt cgctcgccct
    19081 acgggccgtt gctggcgcaa cgttatcctt cacgcttaac atctgagttt gatgttaaaa
    19141 ctggtgggtc gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc
    19201 aactgagcta acgacccgag tggtgggtga tgacgggatc gaaccgccga ccccctcctt
    19261 gtaagggagg tgctctccca gctgagctaa tcacccgata ctgcgctgaa tactacaaac
    19321 tacttccacc gaacccaccg taattagtgg tgggtcgtgc aggattcgaa cctgcgacca
    19381 attgattaaa agtcaactgc tctaccaact gagctaacga cccggtggtg ggtgatgacg
    19441 ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc
    19501 cgatactacg ctggatacta caactaacta ggttagtggt gggtcgtgca ggatgactcg
    19561 acttcgtctc gcccttcggg ccgttgctga cgcaacgtta tccttcacgt tttactaccg
    19621 cttccacctt agcgattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    19681 gtcaactgct ctaccaactg agctaacgac ccactttttc gttgctttcg gtttgtttga
    19741 tatcccgtgg caacggcggc atatattact gatttcagac ttgagcgcaa caaaaatttc
    19801 gatgcagatc actcaactgc ttatgattcg cacgacacga ccagaaataa tgcgacttct
    19861 ggtcgtgtgt tatgcattac atcgcgttca gacgtttttg cgcctgttta gcgccatcag
    19921 taccagggta tttactgata acctgctggt atacggcttt cgcttttgcg gtgtcacctt
    19981 tgtcctgcat gatgacgccg actttaaaca tcgcatctgc agccttcggt gacttcggat
    20041 agtttttcac taccgaagca aaataatacg ccgcatcatc ttttttaccc ttgttgtagt
    20101 ttaactgacc cagccaataa ttggcgtttg gcaggtaagt tgaatcaggg taatttttga
    20161 tgaaattctg aaatgccact attgcttcat cctggcggga tttatcctgc accagcgcaa
    20221 ttgctgcatt gtaatccgtg tttgcatcgc cgcttttcac cggcgcgcca gcattcgcag
    20281 taccagcatc agctgtcggc gtcgttgatg ccgccgcacc gctttgatca ccgctggttg
    20341 attgcgccgc tgcaccaccg ctgctgaggc tatcgatctg caacaggatc tgcttttgcc
    20401 gctccacgac ctgattcagt tgatactgat tttcctgaat ctgaccgcgc agggaatcaa
    20461 tatcggattg attatcagag agttgttgct ggagttgggt taaaagctgg ctgtgagcat
    20521 ttgaaatacg ctcaagttga gtgacgcggt cttcgaccga gcctgagccg acactactga
    20581 ttggtgcctg agcaaaagcg gcccaggggg ccgctatacc aaccagtaac gacagactca
    20641 atagttgatg tctgaagtta ctgctcatgc aattctctta gtaaaccagt accgcacgac
    20701 ggtttttgga gtatgccgct tcgtcatgac ccagtactgc aggtttttct ttaccgtaag
    20761 aaacgatgga gatctggtct gcagaaacgc ctttaccctg caggtacatc ttaacggcgt
    20821 tcgcacgacg ttcacccagg gagatgttgt attccggagt accacgttcg tccgcgtgac
    20881 cttctacggt gactttgtaa gacgggttgc tacgcaggaa gtttgcatgt gcatccagca
    20941 tttgagcgaa gtcagaacgg atatcgtact tgtccagatc gaagtaaacg atgttgttct
    21001 gctgcagctg ttgcatttgc agacgagcct gctcttcgga agacatgttg ccgttgccgc
    21061 cgttcgcatc cataccagtg ccggcaccca gcatgccttc gctgccgtca ttgctggcgt
    21121 tcttattgga agaacatgcc gcaattgcca taacaggcag agcaatcatc agccctttca
    21181 gcactttgtt cagttgcatt tcaatgattc ctttactatt caattaatta ttatcacaga
    21241 tacggcgacc aggcagggaa tttgacctgt ccatcagttg ccggaagacg cgctttgaaa
    21301 cgcccatctg tagaaaccaa attcagcacg gatcccatcc cctgagaaga gctgtagatt
    21361 accatagtgc cgttaggtgc cagacttggc gtttcatcca ggaacgtgga cgacagaact
    21421 tgtacgcctc ccgttgccag atcttgtttg gcaatgtgct gctgcccacc attggagctg
    21481 accattacca taaatttacc gtcgctgctg acatccgcat cctggttctg cgaaccttcc
    21541 caggtaatac gttgtggcgc accgccgttg atattcactt tataaacctg tggacgaccg
    21601 gcctggtcag aagtaaatgc caggttctgg ctgtccggga accaggtcgg ttcggtattg
    21661 ttactgcgac catccgtcac ctggcggatc tgaccagaag ccaaatccat tacgtacagg
    21721 ttcagactac cggttttcga caaggcgaat gccagtttgc tgccgtctgg cgagaatgca
    21781 ggtgcaccgt tgtgacgcgg gaatgaagcc acctgacgta cagcgccatt tgccagcgtt
    21841 tggatgacca gcgcggaacg accgctttcg aaggtcacat aagccagttt agaaccgtct
    21901 ggtgaccacg ccggagacat cagcggctgc ggtgaacggt gaacgacaaa ctggttgtaa
    21961 ccgtcatagt caaatacgcg cagttcatac gggaactgac cgccgttggt ctgaacaacg
    22021 taggcaatac gggtacggaa cgcaccttta atgccggtca gtttttcaaa cacttcatca
    22081 ctggcggtat gaccagcata acgcagccac tgcttgttca ctttgtacga gttctgagca
    22141 agtacagtac ccggtgcgcc gccagtgtca acaagttgat aagcaacatt gtaagaacca
    22201 tccggattcg gagtgacctg accgacaact acagcgtcaa tacccagcgc ggaccatgca
    22261 gctggttgta cttcctgcgc actacccggc tgctgtggca gacgagcgcg atctaacgga
    22321 ttaaatttac cgctgttacg caagtcggca gcaacgatgc cgccaatatc ttcaggtgcc
    22381 gcaccagggc ccgcccactg gaaaggaaca actccaatag gacgaccgga atctacaccg
    22441 ctgtcgatca caatgcggac ttcagcatgc agaactgatg cccacagtat gagaaaacca
    22501 aatgctactc gtaatgcctg cttcatcata tctcccttat ctggaccgat ggcccacgat
    22561 aatttagcag aatgttaaca aactaaaata cacaaaacta ccagaacccc gtggcaaccg
    22621 gtgcctgatg ttgaccgtcg gaacagtcaa catcgcgatt acggtttgaa gtccaatggc
    22681 gcgtttttga acacttcata tactgcctgg cttggtggtt tcgggatctt cgcaagttta
    22741 gctgctgcca acgcagcctg acaaagtgcg ggatcgccac cttcaggttt gatatccagt
    22801 aacataccat cgggagccag ttttatgcgc agcgtacagg ttttgcctgc ataggacgat
    22861 gcgtcataga acttactttc gatagcagat ttaatctgcc cggcatagtt attgatatcg
    22921 gcccctgatg cgccattgtt tttagtatta ccactcccgg ctggcgaagc attgttccct
    22981 ttcgcccctc cccccgtttt cggtgcattc ttaccagagc ttagctcacc gaaaatatca
    23041 tctgcctctg cggcagcttt tgctgcagcg gctttttcag ctgctgcttt tgcagctgcc
    23101 gcttttttgt ctgcggcggc tttttctgcc gctgctttct tatcagctgc tgccttttca
    23161 gcagccgctt tttctgctgc ttttttgtcg gctgcagctt tctctgctgc cgctttctta
    23221 tcagctgcag ccttttcagc agccgctttc ttctcagctt ctgctttggc tttttcagca
    23281 gcttcagttg ccgctttctt tcttgcttca gctgcagctg cttctgccgc ttccgctttc
    23341 ttcttcagtg ccgccgcggc tgcctcggct tttttctgcg cttcggctgc ggctttggcg
    23401 gcttctgctt ctgctttttt cttcgcgtct gcagccgctt tcttcgcagc ttcttccgca
    23461 gctttagcat ccgcttcggc cttcgcttta gcatctgccg ccgctttcgc tgccgcttct
    23521 tccgcttgct tctgctttaa ctcggcctgt tttgcggctt cttcagcctg ctttttctgc
    23581 tcctgagcag ctaaccgctc tttctcaagt tgcttcaggc gttcctgttc agccgcttgt
    23641 ttctcacgca gctcttccgc agcctgctgt tccttcatct tgcgctgctc atcagaacgc
    23701 ttcgcgcttg attcctggct ttgcatgcgt ttgtactgct caactaccgc acctgaatca
    23761 accatgacag cgtcgatgga tgaaccaccg ccgcctccgg ctgaagcttc tatattctca
    23821 tcgaacgaac tccagatcag cgccgcaaat aagatgacat gcagcactgc tgaaataatt
    23881 atcgcccgct tgagcttgtc gttttgttcg gttgcctttg acactctcgg tttccaaaaa
    23941 ctgttcgcct gttacccgct ctctttcaag caagggaaac gcagatgttt agataggctg
    24001 cgtcattaaa ccaaccgatt tcacacccgc actatgtaac aagttcagtg ctttaattat
    24061 ttcatcgtaa ggcacatctt ttgcgccacc gatcagaaag accgttttcg ggttggcctt
    24121 gaaacggctg gacacttccg ccaccacctg ctctggtggt aaacgctcca ggcgatcttt
    24181 ctcaaccacc acggtgtact gaccaatacc agacacttca acaatcactg gcggattatc
    24241 gttactgctc accgcctgtg attcagtagc gtctggcaga tcgacctcca cgctctgggt
    24301 gatgatgggc gctgtcgcca taaagatcag caacagcacc agcagtacgt ccagcaacgg
    24361 tacaatgttg atttcggact tgagatcgcg acgacctcgt ccacgcgctc tggccatggc
    24421 ttaccccttg ttgctctcgc taacggtaaa cgcctggcgg tgcagaatcg cggtaaactc
    24481 ttccataaag ttgtcgtaat tcagttccag tttgtttacg cgctggttga gacggttata
    24541 agccattacc gccggaattg cggcgaacag accaattgcc gtcgcaatca acgcttctgc
    24601 gataccgggc gcaaccattt gcagtgttgc ttgttttacc gccccgaggg cgataaaggc
    24661 gtgcatgatc ccccagaccg taccaaacag accaatatac gggctgatgg agccaaccgt
    24721 accgaggaac ggaatgtgcg tttccagatt ttcaagttca cggttcatgg agatacgcat
    24781 agcacgcgac gccccttcca ctacggcttc cggcgcatgg ctattggcac gatgcaggcg
    24841 cacaaactct ttgaacccgc tgtaaaagat ttgttccgaa ccagtcagat tatcccgttt
    24901 cccctgactc tcttgataga ggcgagagag ttcgattcca gaccagaatt tatcttcaaa
    24961 cgcttcggct tcacgcgccg ctgcgttaag aatacgggtc cgctggataa taatggccca
    25021 ggatgcgatt gaaaaaccaa tcaaaatcaa catgataagt ttaaccagaa ggctagcctt
    25081 caggaacaaa tcaaggatat tcatgtcagt cactgcttaa actccgcgac aatagacttg
    25141 ggaagcgcac gaggcttcat tttgagtggg tcaacgcaaa caaccagaac ctctgcttca
    25201 ttcagcaaag tattctcggc gttgacaata cgttgcgtga aaaccaaaga ggtgccacgc
    25261 attgatgtta tttcagtctg tatttcgagc atatcgtcga gccgcgcagg tgcgtaatat
    25321 tccaccgtca ttttacgtac cacaaaggca acgcgttcag ccatcagcgc ctgttgactg
    25381 aagtgatgat gacgcagcat ctctgtgcgt gctctttcat aaaaagcgac gtaactggcg
    25441 tggtacacca caccaccggc atcggtatct tcatagtaga cgcgaaccgg ccatcgaaac
    25501 agcgttgtat tcactttaca tcccggtaat gcaacaaaag ttagagcttt taaacgctgc
    25561 tactatacgc gcgcgattga ggtttgggaa tgagggcaag ttaagggagc gtaaaaattt
    25621 atgggcctgg agcggcccat aattttttaa agagaattct gatttaaaag aagaagaaaa
    25681 tcagcccgac aatgagaaca atatcggcaa gcaatgggca aaaaatccct tgccaaagaa
    25741 ccttctgcgg acgaaagccc acgccgtgaa tcacaccagc acagacggcc cacatcagca
    25801 ataaaccatg ccagatttcc agttcactgg tcttcgcggc gaaacgtgat gggtcccaaa
    25861 acatacatcc tgctaacaga agcgccatca cgaaggaaag cgcccgcagg gggcgcttgt
    25921 ccattaccgc atacaaggtt gcgataatct tactcatcag atgtcttctt gaccggcttt
    25981 gcctgattcg acgtgctcaa gcgccagcgc ggtgattacc ccaaacgaac aggcaagaag
    26041 cgttcccaga atccatgcga aataccacat ttttagctcc ttacttagta cagagagtgg
    26101 gtgttacgtt caatatcttc tttggtgata cgaccgaaca ttttccagta acaccaggcg
    26161 gtgtagagca gaatgatcgg taccagaacc accgcaaccc aggtcatgac gttaagcgtc
    26221 agctggctgg aagttgcatc ccacattgtc agacttgcgt tcatcatggt gctggacggc
    26281 atcacaaacg ggaacattgc gataccggct gtcaggatga tgcaggccag cgtcagggag
    26341 gagaacacaa acgcccacgc cgctttatcc atacgtgcag tcaggatggt cagcagcggc
    26401 agaaccacac ccagtgccgg aatagcccac agaattggcg tgttgttgaa gttaaccagc
    26461 catgcgccag cttcacgaac cacttcttta ttcagtgggt tagaggctgc gtaatggtcc
    26521 attgtcgatt tcacgacata accatcgata ccgtacatca cccatacgcc agccagtgcg
    26581 aaacagacca gtgtcaccag tgccgccacc tgagccgttg cacgggtacg caggtgcagt
    26641 tcgcccacgg tacgcatttg cagataggtt gcgccctgag taatgatcat ccctacgctc
    26701 accacgcctg ccagcaggcc gaacgggtta agcaggtgga agaagttacc ggtgtagtac
    26761 agacgcagat attcatcaac gttgaacggt acgccctgca acaggttacc gaacgctaca
    26821 ccaatcacca acggcggaac gaagctacca atgaagatgc cccagtccca catgttacgc
    26881 cagcgggttt cttcaatctt ggagcggtag tcaaaaccga ccggacggaa gaacaaagac
    26941 gccagcacga ggatcatcgc cacatagaag ccggagaacg cagcggcata gaccatcggc
    27001 caggcagcaa agagtgcgcc gcccgcggtg ataagccata cctggttacc gtcccagtgt
    27061 ggtgcaatgg agttaatcat aattcgacgc tcggtgtcgt tacgaccgag gaaacgagtg
    27121 agcatgccca cccccatgtc gaaaccgtca gtgactgcaa aaccaatcag cagaacgcca
    27181 accagcagcc accagataaa acgcaatact tcataatcga tcatttgacg actcctgtct
    27241 tagcgtgccg gctgagtagt cgtggaagac tgctcaaagt gatagcgacc ggttttcagg
    27301 ctgcttgggc cgaggcgtgc aaatttgaac attaagaaca attctgccac caggaacagg
    27361 gtatacaggc cgcaaatcag caccattgag aagatgagat cgcctgcggt cagtgacgag
    27421 ttcgccacag ctgtcggcag cacttcaccg atagcccacg gttggcgacc atattcagcc
    27481 acgaaccagc ccgcttctac agcaatccac ggcagcggaa taccgtacag cgcggcgcgc
    27541 agcagccatt ttttctcgcc aatgcggttg cggatgacac tccagaaaga gagcgcgatg
    27601 attgccagca gcaggaagcc acacgccacc atgatacgga acgcgaagta cagcggcgct
    27661 acacgcggaa tggagtcttt ggttgcctgt tgaatctgcg cttcagtcgc atcagccacg
    27721 tttggcgtat agcgtttcag cagcagaccg taaccgaggt ctttcttcat gctattgaac
    27781 tggtcacgaa ccgcctggtc ggtagaacca gaacgcagtt gctcgagcag agagtacgcc
    27841 ttcatcccgt tacgaatgcg ttcttcatgc tgcaccatca gttctttcag gccgataact
    27901 ggagtatcca cggaacgcgt tgcaatgatg cccagcgcgt aagggatctg gatcgcaaat
    27961 ttgttcgtct cctcttcctg atcaggaatg ccgaacagag taaaggcagc aggcgcaggt
    28021 tgcgtttccc actcggcttc aatagcagcc agtttggttt tctgcacgtc gcccatttcg
    28081 taaccagatt catcacccag aacaataaca gacagaacgg cagccatacc gaagctggca
    28141 gcgatagcaa aagagcgttt agcgaaggcg aagtcacgac ctttcagcat ataccatgcg
    28201 ctgataccga ggatgaacat cgcgccagtc acataaccag acgctacagt gtgaacgaat
    28261 ttcacctgag caaccgggtt aagcaccagc tcggagaagc tcaccatctc catacgcata
    28321 gtttcaaagt tgaaatcgga cgcgattggg ttttgcatcc agccgttcgc aaccagaatc
    28381 cacagtgcgg acaggtttga accgagcgcc accagccagg tgacacacat atgctgaact
    28441 ttacccagac gatcccaacc gaagaagaac agacctacaa aggtggattc gaggaagaag
    28501 gccatcagac cttcgattgc cagcggcgca ccgaagatat cccctacata gtgggaatag
    28561 taagaccagt tagtcccgaa ctggaactcc atggtcagac cggtagccac acccagagcg
    28621 aagttgatac caaacaactt gccccagaac ttggtcatat ctttataaat ctgtttgccg
    28681 gaaaggacgt agaccgtttc cataatggcc agcaggaacg ccataccgag cgtcagtggc
    28741 acaaaaagga agtggtacat cgcggtcaag gcaaactgta agcgcgacag ttcgactata
    28801 tctaacatca tgactccttg ctcatcgcat gaagactccg agagtgaacc ccgttagaaa
    28861 gggtcacacg catgccccaa tacaaaatta tttgctcgcc tctatcgttg ccatttcatc
    28921 aaaagaggaa aagacaatga taaaaaagtt acaaatgtgt taatgaataa accaaattga
    28981 tcccgtaatt atattacgcc gcaaaatcct tacaataaac aggtttttat tgagcaaatt
    29041 tgcatttttc gacagtgatc aatttatagc gaaattgtca cgatctcacc aatttgatct
    29101 acgacaattt atcgtgtttt gcagattttt ttcaag
//
LOCUS       DAZKGF010000046        28534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZKGF010000046 DAZKGF010000000
VERSION     DAZKGF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.046"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            45..1451
                     /gene="fliD"
                     /locus_tag="ACHOQV_001937"
     CDS             45..1451
                     /gene="fliD"
                     /locus_tag="ACHOQV_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP9630314.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLIIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            1476..1886
                     /gene="fliS"
                     /locus_tag="ACHOQV_001938"
     CDS             1476..1886
                     /gene="fliS"
                     /locus_tag="ACHOQV_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP9630315.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPD"
     gene            1886..2251
                     /gene="fliT"
                     /locus_tag="ACHOQV_001939"
     CDS             1886..2251
                     /gene="fliT"
                     /locus_tag="ACHOQV_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFP9630316.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            2329..3816
                     /gene="amyA"
                     /locus_tag="ACHOQV_001940"
     CDS             2329..3816
                     /gene="amyA"
                     /locus_tag="ACHOQV_001940"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP9630317.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(3850..4263)
                     /gene="yedD"
                     /locus_tag="ACHOQV_001941"
     CDS             complement(3850..4263)
                     /gene="yedD"
                     /locus_tag="ACHOQV_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFP9630318.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     SALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            4450..5655
                     /gene="yedE"
                     /locus_tag="ACHOQV_001942"
     CDS             4450..5655
                     /gene="yedE"
                     /locus_tag="ACHOQV_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFP9630319.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            5652..5885
                     /gene="yedF"
                     /locus_tag="ACHOQV_001943"
     CDS             5652..5885
                     /gene="yedF"
                     /locus_tag="ACHOQV_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFP9630320.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            5994..6662
                     /gene="yedK"
                     /locus_tag="ACHOQV_001944"
     CDS             5994..6662
                     /gene="yedK"
                     /locus_tag="ACHOQV_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFP9630321.1"
                     /translation="MCGRFAQSQTREDYLAFLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAVREWMRQEVGGKEASEIAASGCVPANQFTWHPVSCAVGNVKNQGAAL
                     IQPV"
     gene            6773..7252
                     /gene="yedL"
                     /locus_tag="ACHOQV_001945"
     CDS             6773..7252
                     /gene="yedL"
                     /locus_tag="ACHOQV_001945"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9630322.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PKVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCENNELP"
     gene            7492..7632
                     /locus_tag="ACHOQV_001946"
                     /pseudo
     CDS             7492..7632
                     /locus_tag="ACHOQV_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(7657..7971)
                     /gene="fliE"
                     /locus_tag="ACHOQV_001947"
     CDS             complement(7657..7971)
                     /gene="fliE"
                     /locus_tag="ACHOQV_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFP9630323.1"
                     /translation="MSAIQGIEGVISQLQATAMSARTQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            8186..9844
                     /gene="fliF"
                     /locus_tag="ACHOQV_001948"
     CDS             8186..9844
                     /gene="fliF"
                     /locus_tag="ACHOQV_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFP9630324.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSEESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            9837..10832
                     /gene="fliG"
                     /locus_tag="ACHOQV_001949"
     CDS             9837..10832
                     /gene="fliG"
                     /locus_tag="ACHOQV_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFP9630325.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            10825..11511
                     /gene="fliH"
                     /locus_tag="ACHOQV_001950"
     CDS             10825..11511
                     /gene="fliH"
                     /locus_tag="ACHOQV_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFP9630326.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGVAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            11511..12884
                     /gene="fliI"
                     /locus_tag="ACHOQV_001951"
     CDS             11511..12884
                     /gene="fliI"
                     /locus_tag="ACHOQV_001951"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFP9630327.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGSALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            12903..13346
                     /gene="fliJ"
                     /locus_tag="ACHOQV_001952"
     CDS             12903..13346
                     /gene="fliJ"
                     /locus_tag="ACHOQV_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFP9630328.1"
                     /translation="MTEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            13343..14470
                     /gene="fliK"
                     /locus_tag="ACHOQV_001953"
     CDS             13343..14470
                     /gene="fliK"
                     /locus_tag="ACHOQV_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP9630329.1"
                     /translation="MIRIAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETPPVINDKQSTSTSLTTAQ
                     TITLAAAADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSSVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            14575..15039
                     /gene="fliL"
                     /locus_tag="ACHOQV_001954"
     CDS             14575..15039
                     /gene="fliL"
                     /locus_tag="ACHOQV_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFP9630330.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            15044..16048
                     /gene="fliM"
                     /locus_tag="ACHOQV_001955"
     CDS             15044..16048
                     /gene="fliM"
                     /locus_tag="ACHOQV_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFP9630331.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            16045..16458
                     /gene="fliN"
                     /locus_tag="ACHOQV_001956"
     CDS             16045..16458
                     /gene="fliN"
                     /locus_tag="ACHOQV_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFP9630332.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            16461..16826
                     /gene="fliO"
                     /locus_tag="ACHOQV_001957"
     CDS             16461..16826
                     /gene="fliO"
                     /locus_tag="ACHOQV_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFP9630333.1"
                     /translation="MNNHATVQSSAPVSTAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            16826..17563
                     /gene="fliP"
                     /locus_tag="ACHOQV_001958"
     CDS             16826..17563
                     /gene="fliP"
                     /locus_tag="ACHOQV_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFP9630334.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLTGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            17573..17842
                     /gene="fliQ"
                     /locus_tag="ACHOQV_001959"
     CDS             17573..17842
                     /gene="fliQ"
                     /locus_tag="ACHOQV_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFP9630335.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            17851..18636
                     /gene="fliR"
                     /locus_tag="ACHOQV_001960"
     CDS             17851..18636
                     /gene="fliR"
                     /locus_tag="ACHOQV_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP9630336.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            18926..19549
                     /gene="rcsA"
                     /locus_tag="ACHOQV_001961"
     CDS             18926..19549
                     /gene="rcsA"
                     /locus_tag="ACHOQV_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFP9630337.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(19593..19781)
                     /gene="dsrB"
                     /locus_tag="ACHOQV_001962"
     CDS             complement(19593..19781)
                     /gene="dsrB"
                     /locus_tag="ACHOQV_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFP9630338.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            19944..20171
                     /gene="yodD"
                     /locus_tag="ACHOQV_001963"
     CDS             19944..20171
                     /gene="yodD"
                     /locus_tag="ACHOQV_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFP9630339.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            20470..21285
                     /gene="yedP"
                     /locus_tag="ACHOQV_001964"
     CDS             20470..21285
                     /gene="yedP"
                     /locus_tag="ACHOQV_001964"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFP9630340.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAKQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(21282..22976)
                     /gene="dgcQ"
                     /locus_tag="ACHOQV_001965"
     CDS             complement(21282..22976)
                     /gene="dgcQ"
                     /locus_tag="ACHOQV_001965"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFP9630341.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     VGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(23147..23329)
                     /gene="yodC"
                     /locus_tag="ACHOQV_001966"
     CDS             complement(23147..23329)
                     /gene="yodC"
                     /locus_tag="ACHOQV_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFP9630342.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(23408..24325)
                     /gene="yedI"
                     /locus_tag="ACHOQV_001967"
     CDS             complement(23408..24325)
                     /gene="yedI"
                     /locus_tag="ACHOQV_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFP9630343.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            24498..25418
                     /gene="yedA"
                     /locus_tag="ACHOQV_001968"
     CDS             24498..25418
                     /gene="yedA"
                     /locus_tag="ACHOQV_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFP9630344.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(25407..25877)
                     /gene="vsr"
                     /locus_tag="ACHOQV_001969"
     CDS             complement(25407..25877)
                     /gene="vsr"
                     /locus_tag="ACHOQV_001969"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFP9630345.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(25858..27276)
                     /gene="dcm"
                     /locus_tag="ACHOQV_001970"
     CDS             complement(25858..27276)
                     /gene="dcm"
                     /locus_tag="ACHOQV_001970"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFP9630346.1"
                     /translation="MQENISVTDSYSTGNVVQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEVKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(27343..28038)
                     /gene="yedJ"
                     /locus_tag="ACHOQV_001971"
     CDS             complement(27343..28038)
                     /gene="yedJ"
                     /locus_tag="ACHOQV_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFP9630347.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQHSSILAAEEARRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(28078..28359)
                     /gene="drpB"
                     /locus_tag="ACHOQV_001972"
     CDS             complement(28078..28359)
                     /gene="drpB"
                     /locus_tag="ACHOQV_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFP9630348.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         6965 a         7483 c         7346 g         6740 t
ORIGIN      
        1 accccggtat tcgttttacg tgtcgaaaga taaaaggaaa tcgcatggca agtatttcat
       61 cgctgggagt cgggtcaggt ctggatttaa gttcaatcct tgatagcctc accgccgcgc
      121 aaaaagcgac gctaacccct atttcaaatc agcaatcgtc gtttaccgct aaacttagcg
      181 cctacggtac gctgaaaagc gcgctgacga ctttccagac cgccaatact gcattgtcta
      241 aagccgatct tttttccgcc accagcacta ccagcagcac caccgcgttc agtgccacca
      301 ctgcgggtaa cgccatcgcc gggaaataca ccatcagcgt cacccatctg gcgcaggcgc
      361 aaaccctgac cacgcgcacc accagagacg atacgaaaac ggcgatcgcc accagcgaca
      421 gtaaactcat cattcaacaa ggcggcgaca aagatccgat taccattgat atcagcgcgg
      481 ctaactcatc gttaagcggg atccgtgatg ctatcaacaa cgcaaaagcg ggcgtaagcg
      541 caagcatcat taacgtgggt aacggtgaat atcgtctgtc agtcacatca aatgacaccg
      601 gccttgataa tgcgatgaca ctctcggtca gcggtgatga tgcgctacaa agttttatgg
      661 gctatgacgc cagtgccagc agcaacggca tggaggtctc ggtcgccgcc cagaatgcgc
      721 agctgacggt caacaacgtc gccattgaga acagcagcaa caccatcagc gacgcgctgg
      781 aaaacatcac cctgaacctg aacgatgtca ccacgggcaa ccagacgcta accatcactc
      841 aggacacctc caaagcgcaa acggcgatta aagactgggt gaatgcctat aactcgctaa
      901 tagatacctt cagcagcctg accaaataca ccgccgtaga tgcgggagct gatagccaga
      961 gttctagcaa tggcgcactg ctcggcgact ccacgttgcg gacgattcag acgcagttga
     1021 aatcgatgct gagtaatacc gttagttctt ccaactataa aacgttggcg cagattggta
     1081 tcacgaccga tcccagcgat ggcaaactgg aactggatgc cgacaaactc accgctgcac
     1141 tgaaaaaaga tgccagcggc gtaggtgcat tgattgttgg cgatggtaaa aaaaccggca
     1201 tcacgaccac catcggcagc aacctgacca gttggctttc gacaacaggc attattaaag
     1261 ccgctaccga tggcgttagt aagaccctga ataagttaac caaagactac aacgccgcca
     1321 gcgatcgcat tgatgcgcag gtcgcgcgct acaaagagca atttacccaa ctggacgttt
     1381 taatgacctc gttaaacagc accagcagct acttaacgca gcagttcgaa aacaacagta
     1441 attccaagta atcaatattc atcgggagac aggtcatgta cgcggcaaaa ggcacccagg
     1501 cctatgcaca aattggcgtc gaaagcgccg taatgagcgc cagccagcaa cagctggtca
     1561 ccatgctatt tgatggagtg ctgagcgcac tggttagagc gcgcctgttt atgcaggaca
     1621 acaatcagca aggcaaaggc gtctctttgt caaaagcgat caacatcatt gagaacgggc
     1681 tgcgggtgag tcttgatgaa gagagcaaag acgaactaac ccaaaacttg attgctcttt
     1741 atagctatat ggtcaggcgc ttgctgcaag ccaatttacg caacgatgtc tccgcagtcg
     1801 aagaagtgga agcattaatg cgcaatattg ccgatgcctg gaaagagtct ttactctccc
     1861 cttctttgat tcaggaccca gactgatgaa caatgcaccg catttgtatt tcgcctggca
     1921 acaactcgtc gaaaaaagtc agctcatgtt acgcctggcg acagaagaac aatgggacga
     1981 actcatcgcc agcgaaatgg cgtatgtgaa tgcggtgcag gagatcgcac acctgactga
     2041 agaggttgcc ccgtccacca cgatgcagga gcagctccgc ccgatgctgc gcctgattct
     2101 cgacaacgaa agcaaggtaa agcagttatt acagattcgg atggatgaac tggcgaaact
     2161 ggtcggtcag tcatcggtgc aaaaatcggt gttaagtgcc tatggcgatc agggtggctt
     2221 tgtgctggct ccgcaggata acctcttttg aatcttaaag agccaatggc tcgcgaataa
     2281 tctgattacg gctacgcttc taatgttccc cttgaatgga gtcgaagaat gcgtaatccc
     2341 acgctgttac aatgttttca ctggtattac ccggaaggcg gtaagctctg gcctgaactg
     2401 gccgagcgcg ccgacggttt taatgatatt ggtatcaata tggtctggtt gccgcccgcc
     2461 tataaaggcg catcgggcgg gtactcggtc ggctacgact cctatgattt atttgattta
     2521 ggcgagtttg atcagaaagg cagcatccct actaaatatg gcgataaagc acaactgctg
     2581 gccgccattg acgccctgaa acgtaatgac attgcggtgc tgctggacgt ggtagtcaac
     2641 cacaaaatgg gcgcggatga aaaagaagct attcgcgtgc aacgtgtaaa tgctgatgac
     2701 cgtacgcaaa ttgacgaaga aatcattgag tgcgaaggct ggacgcgtta caccttcccc
     2761 gcgcgtgccg ggcaatactc gcagtttatc tgggatttca aatgttttag cggtatcgac
     2821 catatcgaaa accctgatga agatggcatt tttaaaattg ttaacgacta taccggagaa
     2881 ggctggaacg atcaggttga tgatgaactc ggtaatttcg attatctgat gggtgagaat
     2941 atcgattttc gcaatcatgc cgtgacggaa gagattaaat actgggcgcg ctgggtgatg
     3001 gaacaaacgc aatgcgacgg ctttcgtctt gatgcggtca aacatattcc ggcatggttt
     3061 tataaagagt ggatcgaaca cgtacaggaa gttgcgccaa agccgctgtt tattgtggct
     3121 gaatactggt cgcatgaagt cgataaactg caaacgtata ttgatcaggt ggaaggcaaa
     3181 accatgctgt ttgatgcgcc gctgcagatg aaattccatg aagcatcgcg catgggtcgc
     3241 gactacgaca tgacgcagat cttcactggg acgctggtgg aagccgatcc ttttcacgcc
     3301 gtaaccctcg ttgccaacca cgacacccaa ccgttgcaag ccctcgaagc accggtcgaa
     3361 ccgtggttta aaccgctggc gtatgcctta attttgttgc gggaaaatgg cgttccttcg
     3421 gtgttctatc cggacctcta cggtgcacat tacgaagatg tcggtggtga cgggcaaacc
     3481 tatccgatag atatgccaat aatcgaacag cttgatgagt taattctcgc ccgtcagcgt
     3541 ttcgcccacg gtgtacagac gttatttttc gaccatccga actgcattgc ctttagccgc
     3601 agtggcaccg acgaatatcc cggctgcgtg gtggtcatgt cgaacgggga tgatggcgaa
     3661 aaaaccattc atctgggaga gaattacggc aataaaacct ggcgtgattt tctggggaat
     3721 cggcaagaga gcgtagtgac cgacgaaaac ggcgaagcga ccttcttttg caacggcggc
     3781 agcgtcagcg tgtgggttat cgaagaggtg atttaaattc atccccggcg gcaagccggg
     3841 gagatttcat tacggcagtg gcgtcggtaa cggcgcttta tccagcgcgg cggcacactc
     3901 tgccgtcggg cgatccacac gctccatcgt cataccatcg tactcaatgg tgttaccgtc
     3961 acgctcaacc tcgtacagct cacgtttcac ggtaacgtta gtgagatcat cagacatcaa
     4021 tgtcaactta cccggcacgg caatcacccg ctgccactga cggcaatcca gggtatcgcc
     4081 ctctttagtg acaatcaggc tgcctatcgc ttccggactg accagcgcac tttgcggccc
     4141 tttggtctgc cagtagcccg ccagccagtc cggcgcgggg gttttcacga cattgttata
     4201 gttttcgact tcggcacagc ccgccagcaa catgagcgcg cctgcaattg cgagtttttt
     4261 catcattcgt cctgcatgag aagaaaagag gattgtggca ttaaagccct gatgtcgcca
     4321 gcctttccag gttcagagag aaatttgatc taactattat ttttgtgaat tattaccccg
     4381 aaaataaatt tgcttgatgt atgattcgca actcttgttt caaaccttct gagttcagag
     4441 gctacataca tgtcatggca gcaattcaaa cacgcctggt tgattaaatt ctgggccccc
     4501 atccctgcgg tcatcgcggc gggtattctc tccacttact actttggcat cactggcacc
     4561 ttttgggctg tcacgggtga atttacccgc tggggcggtc agcttctgca gctgttcggc
     4621 gtccatgctg aagagtgggg ttattttaaa attatccatc tggaaggatc gccattaacc
     4681 cgcatcgacg gcatgatgat cctcggtatg tttggcggct gctttgctgc agcgctgtgg
     4741 gccaacaatg tcaaactacg catgccacgc agccgtatcc gcattatgca ggccattatt
     4801 ggcggcatta tcgctggttt tggcgcgcgt ctggcaatgg gctgtaacct ggcggcgttc
     4861 tttaccggca ttcctcagtt ctcgctgcat gcctggttct ttgccatcgc cactgccatt
     4921 ggttcatggt ttggcgcgcg ctttaccctg ctgcccatct tccgtattcc cgtgaaaatg
     4981 cagaaagttt ctgccgcctc accgctgacg caaaaaccgg atcaggcgcg gcgtcgtttt
     5041 cgtctcggga tgctggtctt tttcggcatg ctgggctggg cgctgctcac ggcgatgaac
     5101 caaccaaaac tggggctggc aatgctgttt ggcgtcggct ttggtttact gattgaacgt
     5161 gcgcaaatct gctttacttc ggcgttccgc gatatgtgga tcaccggacg tacccatatg
     5221 gcgaaagcaa tcattatcgg tatggcggtg agtgccatcg ggatcttcag ttacgtacag
     5281 ttaggcgttg aacctaaaat catgtgggcg ggaccaaatg cggtaattgg tggtttactt
     5341 tttggttttg gcatcgtgct ggcaggcggc tgcgaaaccg gctggatgta ccgcgcggta
     5401 gaaggccagg tgcactactg gtgggtcggt ctgggcaacg tgatcggctc aacgattctg
     5461 gcgtattact gggatgattt cgctccggcg ctggccaccg actgggacaa aatcaacctg
     5521 ctgaaaacct ttggtccgat gggtggcctg ctggtgacat atttgctgtt gtttgcagct
     5581 ctaatgttga ttatcggctg ggaaaaacgc ttcttccgcc gtgcggcacc gcagattgct
     5641 aaggagatcg catgaaaaat atcgttcctg attaccgtct tgatatggtg ggtgaaccct
     5701 gcccttatcc ggctgtcgca acccttgagg cgatgccgca gttaaaaaaa ggggagatcc
     5761 tggaagtggt gagcgactgt ccgcagtcga tcaataatat tccactggat gcgcgtaatc
     5821 acggctatac ggtgctggat attcagcaag acgggccgac cattcgttat ttaattcaga
     5881 agtaatccct cattcccgct gtggcccaca gcgggaacat cgttaccact ctatcacata
     5941 gattttaccc gccccctctc ctattctttt cagtgtgctc tcaggaggtt tctatgtgtg
     6001 gacgctttgc ccaatcccaa acgcgtgaag attacctcgc gtttctcgcg gaagatattg
     6061 aacgcgatat tccctacgat cccgaaccta ttggcagata caacgtcgcg ccgggaacca
     6121 aagtccttct gctcagtgaa cgtgatgaac accttcatct ggatccggtt ttctggggat
     6181 atgctcccgg atggtgggat aaaccgccgc tgattaacgc ccgcgtagaa actgccgcaa
     6241 ccagtcgcat gtttaaaccg ctctggcaac acggtcgggc aatctgtttt gccgatggct
     6301 ggtttgagtg gaaaaaagaa ggcgacaaaa aacagcctta ttttatctat cgcgctgacg
     6361 gacaacctgt ttttatggcc gcgataggta gcacaccgtt tgagcgtggt gacgaagccg
     6421 aaggattttt gatcgtcact gcggcggcag atcaaggtct ggtagatatt catgaccgcc
     6481 gcccgctggt tctgtcgcca gaagctgtac gggaatggat gcggcaagag gttggcggta
     6541 aagaagcctc agaaattgct gccagtggct gtgtgccagc aaaccagttc acctggcacc
     6601 cggtatcgtg cgcggtgggt aatgttaaaa accagggagc ggcgttaatt caacctgttt
     6661 gataagcctg gagattattg atcaacaata ctgcgtcata agaaatctct attagacaaa
     6721 gatttcagta cctgttggca tattgcaaaa ataacaccaa tacggaatcg tcatgttcac
     6781 gattaaaaca gatgatctca cccatccagc agtgcaagca ttagtggctt accatatttc
     6841 cggcatgctg cagcagtctc cccccgaaag tagtcatgct ttagatgtgc aaaaattacg
     6901 taacccgaaa gtgacattct ggtcagtatg ggaaggcgaa caactcgcag gaattggtgc
     6961 gctgaagttg ctggatgata aacatggcga actgaaatca atgcgtaccg cgccaaatta
     7021 tttacgtcgg ggtgtcgcca gtctgatttt acgccacatt ttgcaggtcg cccatgacag
     7081 atgccttcat cgcctgagct tagaaacggg tacacaggct ggatttacgg cctgccatca
     7141 actttatttg aaacatggat tcgttgattg cgaaccgttt gctgattatc aacttgatcc
     7201 gcacagtcga tttttgtcat tgacgctatg cgaaaataat gaattgccat gagccagacg
     7261 cagcacattc ttgcattcga cgtgctgcgc ctttatttat tatcagccgc aaatgtcttg
     7321 tccttagacg ctctttccac atgcgccaca agaaacctct attccagtga cacaattatg
     7381 cctgattatt tccctagccc ggaatatgaa taaaaacatc aaataagatg aaattatcaa
     7441 aatattaatg ttgcgtctga ggtcgtaaca acatccgacg tggtgatatg tcaaatgaga
     7501 cactctgatg gtcaaagggt ttatcgcgtt tacagatcgt ggatggcaga aaactttcag
     7561 aatcaggttt ccattttaaa ccagaaatta agtgagtttg tcccatccat gccccacgcg
     7621 gtgagatcgg atgtaatcaa caacaggtta caaaacctac acctgcatgc tcatcacttc
     7681 ctgatacgcc gccaccagct tattacgcac ctgaatcccc atttgcattg aaactgaggc
     7741 tttttgcata tcggtcatca catcgtttaa cgccacgccg ggttcaccga gagtgaattt
     7801 ttctgcctgc gtgcgggcgg ctgtttgtgt atcgcttatg cgatcgagcg cggcgtgcag
     7861 ctgcccggca aaactaatgg tcggttgtgg cagtgattcc tgtgtacgcg cactcatcgc
     7921 cgtagcctgt aactggctga taaccccttc aatcccctgt atcgctgaca ttttcatctc
     7981 ccggataatt tctggcagca aagcctacca gtaagtcaat aagacaaagg cgctaaatag
     8041 caacaaaaaa acgggtttat tggcggatag aaaaaaacga aagcacaaat aatgggagcg
     8101 tcaatttttc gagtttgctg acccgggagt gagtcttgtt ccactttgcc aataacgccg
     8161 tccataatca gccacgaggt gcgcgatgaa tgcgactgca gcccagacaa aatctcttga
     8221 gtggcttaat cgcctgcgtg cgaatccgaa aattccattg attgttgccg gctccgcagc
     8281 agtggcggtc atggtcgcac tgatcctgtg ggcgaaagcc cccgactacc gcacattatt
     8341 cagcaatctt tccgatcagg atggtggcgc aattgtcagc caactgacgc aaatgaatat
     8401 tccttaccgc ttcagcgaag ccagcggcgc tattgaagtt ccggcagata aagttcacga
     8461 actgcgcctg cgcctggcgc aacaaggttt gcccaaaggc ggcgcggtcg gtttcgaact
     8521 gctcgatcag gaaaagtttg gtatcagcca gttcagcgaa caggtgaatt atcagcgggc
     8581 gctggaaggc gagctttctc gtaccatcga aactatcggc ccggtaaaag gggcgcgcgt
     8641 acatctggca atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc cttctgcatc
     8701 ggtgacggta aatctgttac ccggccgcgc actcgatgaa gggcaaatta gcgccattgt
     8761 acatctggtt tccagcgcgg ttgctggtct gccgccgggg aacgtcacgc tggtggatca
     8821 gggtggacat ctgttaaccc agtccaatac cagcgggcgc gatcttaatg acgctcagtt
     8881 gaaatatgcc agcgatgtcg aaggccggat tcagcggcgt attgaagcga tcctgtcgcc
     8941 aattgttggt aacggtaata ttcacgccca ggtcacggcg caactggact tcgccagtaa
     9001 agaacaaaca gaagaacagt atcgccctaa cggtgatgaa tctcaggcgg cgcttcgttc
     9061 acgccagctt aatgagagcg agcaaagcgg ttccggttat ccaggcggcg taccgggggc
     9121 gttgtcgaat caaccggcac ctgcgaataa cgcgccaatc agcacgcctc cggcaaatca
     9181 aaataaccgc cagcagcagg cgagcaccac cagcaatagt gggccgcgta gcacacagcg
     9241 taatgaaacc agtaactacg aagtcgatcg caccattcgt cataccaaaa tgaacgtggg
     9301 cgatgtgcaa cgtctgtcag tcgcggtagt ggtgaattac aaaaccttgc cagacggtaa
     9361 accgttgcct ctcagcaacg aacagatgaa gcaaattgaa gatctgaccc gcgaggcgat
     9421 gggcttttct gaaaaacgcg gtgactcgct caatgtcgtt aactcgccgt tcaatagtag
     9481 tgaggaaagc ggcggagaac tgccgttctg gcaacaacaa gcgtttatcg atcagttact
     9541 tgctgccggt cgctggttgc tggtactgct ggtggcgtgg ctgctgtggc gtaaagcggt
     9601 acgcccgcag ctaacacgtc gcgctgaggc gatgaaagct gtacagcaac aggcgcaggc
     9661 ccgcgaggaa gtggaagatg cggtggaagt ccgcctgagc aaagacgaac aactccaaca
     9721 acggcgcgct aaccaacgtc tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc
     9781 tgataacgat ccgcgcgtgg tggcgctggt cattcgccag tggataaata acgatcatga
     9841 gtaacctgac aggcaccgat aaaagcgtca tcctgctgat gaccattggc gaagaccggg
     9901 cggcagaggt gttcaagcac ctctcccagc gcgaagtgca aactctgagc gctgcaatgg
     9961 cgaacgtcac gcagatctcc aacaagcagc taaccgatgt gctggcggag tttgagcaag
    10021 aagctgaaca gtttgccgca ctgaatatca acgccaacga ttatctgcgt tcggtattgg
    10081 tcaaagctct gggtgaagaa cgtgccgcca gcctgctgga ggatattctc gaaacccgcg
    10141 ataccgccag cggtattgaa acgctcaact ttatggagcc gcagagcgcc gccgatctga
    10201 ttcgcgatga gcatccgcaa attatcgcca ccattctggt gcatctgaag cgcgcccagg
    10261 ccgccgatat tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg
    10321 ccacctttgg cggcgtgcag ccagccgcgc tggcggaact gaccgaagta ctgaatggct
    10381 tgctcgacgg tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa
    10441 ttatcaacct gatgaaaacg cagcaggaag aagccgttat taccgccgtg cgtgaattcg
    10501 acggcgagct ggcgcagaaa atcatcgatg agatgttcct gttcgagaat ctggtggatg
    10561 tcgatgatcg cagcattcag cgtctgttgc aggaagtgga ttccgaatcg ctgctgatcg
    10621 cgctgaaagg ggccgagcag ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg
    10681 ccgccgatat tctgcgcgac gatctcgcca accgtggtcc ggtgcgtctg tcgcaggtgg
    10741 aaaacgaaca gaaagcgatt ctgctgattg tgcgccgcct tgccgaaacc ggcgagatgg
    10801 tgattggcag cggcgaggat acctatgtct gataatctgc cgtggaaaac ctggacgccg
    10861 gacgatctcg cgccaccaca ggcagagttt gtgccaatgg tcgagtcgga agaaaccatt
    10921 attgaagagg ccgaacccag ccttgagcag caactggcgc aactgcaaat gcaggcccat
    10981 gagcaaggtt atcaggcggg tgttgccgaa ggtcgccagc aaggtcataa gcagggctat
    11041 caggaaggac tggcccaggg gctggaacaa ggtctggcag aggcgaagtc tcaacaagcg
    11101 ccaattcatg cccggatgca gcaactggtc agcgaatttc aaactaccct tgatgcgctt
    11161 gatagtgtga ttgcttcgcg cctgatgcag atggcgctgg aggcggcacg tcaggtgatc
    11221 ggtcagacgc caacggtgga taactcggca ctgatcaaac agatccaaca gttgttgcag
    11281 caagaaccgt tattcagcgg taaaccacag ctgcgcgtgc acccggatga tctgcaacgt
    11341 gttgatgata tgctcggcgc aactttaagt ttgcatggct ggcgcttgcg gggcgatccc
    11401 accctccatc ctggcggctg taaagtctcc gccgatgaag gcgatctcga cgccagtgtc
    11461 gccactcgct ggcaagaact ctgccgtctg gcagcaccag gagtggtgta atgaccacgc
    11521 gcctgactcg ctggctaacc acgctggata actttgaagc caaaatggcg cagttgcctg
    11581 cggtacgtcg ctacgggcga ttaacccgcg ctaccgggct ggtgctggaa gccaccggat
    11641 tacaattgcc gctcggcgca acctgtgtca ttgagcgcca gaacggcagc gaaacgcacg
    11701 aagtagaaag cgaagtcgtt ggctttaacg gtcaacggct gtttttaatg ccgctggagg
    11761 aagtcgaagg tgtcctgccc ggcgcgcgtg tttatgccaa aaacatttcg gctgaagggc
    11821 tgcaaagcgg caagcagttg ccgctcggtt cggcgttatt aggtcgcgtt ctggacggca
    11881 gcggtaaacc gcttgatggc ctcccctccc ccgatacgac ggaaaccggt gcgctgatta
    11941 ccccgccatt taacccgttg caacgtacac cgattgaaca tgtgctggac accggcgtgc
    12001 gcccaatcaa tgccctgctt accgtcgggc gcgggcagcg tatggggctg tttgccgggt
    12061 ccggcgttgg taaaagtgtg ctgctgggga tgatggcacg ttacacccgc gccgatgtca
    12121 ttgtcgtggg tttgattggt gaacgagggc gcgaagtaaa agattttatt gagaatatcc
    12181 tcggtgccga aggacgtgca cgctccgtgg tgattgccgc tccggcggat gtttctccgc
    12241 tcctgcgaat gcagggtgcc gcctatgcca cgcgcattgc cgaagatttt cgcgatcgtg
    12301 gtcagcatgt gttgctgatt atggactccc tcacccgcta cgcgatggcc cagcgtgaaa
    12361 ttgcgctggc gattggcgaa ccacctgcca ctaaaggtta tccaccgtcg gtgtttgcca
    12421 aattaccggc actggtcgag cgtgccggaa atggcattag cggcggcggc tcgattaccg
    12481 cgttttatac cgtgctcacc gaaggcgatg accagcagga ccccattgcc gactccgcgc
    12541 gggcaatcct cgacggtcac attgtgctgt ctcgccgact ggcggaagcc gggcactatc
    12601 cggctatcga tattgaagcg tcgatcagtc gcgcaatgac ggcgttgatc agtgagcaac
    12661 attacgcgcg agtgcgcacc ttcaaacagc tgttgtcgag ttttcagcgt aaccgcgatc
    12721 tggttagcgt cggcgcgtat gccaaaggca gcgatccgat gctcgacaaa gccatcgccc
    12781 tgtggccgca gctggagggc tatttgcaac aaggcatttt tgaacgcgcg gactgggaag
    12841 cgtctctcca ggggctggag cgtattttcc cgacagtatc ataacccagg agataacggc
    12901 agatgacaga acatggcgcg ctggcgaccc tgaaagatct ggcagaaaaa gaggtagagg
    12961 atgccgcgcg cctgctgggt gaaatgcgtc ggggatgtca gcaggcggaa gaacagctca
    13021 aaatgctgat cgattatcag aatgaatatc gcaataacct caatagcgat atgagtgccg
    13081 ggatgaccag caaccgctgg atcaactatc agcagtttat ccagacgctg gaaaaagcca
    13141 ttactcagca tcgccagcaa cttaatcagt ggacgcagaa agtagacatt gccctgaaca
    13201 gctggcgaga aaagaaacaa cgtttgcagg cttggcagac actgcaggaa cggcaatcca
    13261 cggcggcact gcttgcagaa aaccgcctcg atcagaaaaa gatggatgag ttcgcccagc
    13321 gcgccgccat gaggaaacct gaatgattcg catagcgccc ttgattaccg ctgacgttga
    13381 taacgccaca ttgcctggcg gcaaagccag cgatgctgca caagattttc tcgcgttgtt
    13441 gagcgaagca ttagcaggcg agacaacaac cgacaaagcg gccccccagt tgctggtggc
    13501 aacagataag cccacgacaa aaggcgagcc gctggtcagc gatattgttt ccgatgcgca
    13561 acaagcagat ttactgatcc ctgtggatga aacaccgcct gtcatcaacg acaaacaatc
    13621 cacatcaaca tcgttaacca ccgctcagac gataacgttg gctgcggcgg ctgacaacaa
    13681 tacggcaaaa gacgaaaaag cggatgatct gaatgaagac gtcaccgcaa gcctgagcgc
    13741 cctttttgcg atgttgccgg gttttgacaa tacgcccaaa gtgactgatg caccgtcaac
    13801 cgtgttaccg acagagaaac caacgctttt cacaaaactg acttctgcgc aactcactac
    13861 agcacagcct gatgacgccc ccggcacacc agctcagcca ttaacaccgc tggtagcaga
    13921 agcccagagt aaagcggaag tcatcagcac gccttcgtcg gtgaccgctg ccgccagccc
    13981 gctaatcact ccacaccaga cacagccact gcccaccgtc gccgcgcctg ttttgagtgc
    14041 accgctgggt tctcacgaat ggcaacaatc attaagccag catatttcgc tgttcacccg
    14101 ccaggggcaa caaagtgcag agttgcgtct gcacccacag gatttaggtg aagtgcaaat
    14161 ttccctcaaa gtggatgata accaggcgca aatccagatg gtttcaccgc atcagcacgt
    14221 acgcgccgcc ctggaagcag cgctgccggt actgcgtacg cagctggccg aaagtggcat
    14281 tcagttaggg caaagcaaca tcagtggcga aagctttagt ggtcagcagc aggccgcttc
    14341 ccaacaacag caaagccaac gcacagcaaa ccatgaacct ctggcggggg aagacgacga
    14401 tacgcttccg gttcccgtct ctttacaagg gcgtgtaaca ggcaacagcg gcgttgatat
    14461 tttcgcctaa cgtcagaggt agcaccgtaa tccgcgtctt ttccccgctt tgttgcgctc
    14521 aagacgcagg ataattagcc gataagcagt agcgacacag gaagaccgca acacatgact
    14581 gattacgcga taagcaagaa aagcaagcga tcgctttgga tcccgattct ggtattcatt
    14641 accctcgcgg cctgtgccag cgcaggttac agctactggc attcgcatca ggttgccgct
    14701 gaagacaaag cgcagcaacg cgtcgtgccc tccccggtct tctatgcgct ggataccttt
    14761 acggtcaatc tgggcgatgc ggatcgcgta ctttatatcg gcataaccct gcgactcaaa
    14821 gatgaagcta cccgctcgcg gctgagtgag tatttgccgg aagtccgtag ccgtttgctg
    14881 ttactgtttt cgcgtcagga tgctgccgta ctggcgacag aagaaggtaa gaaaaacctg
    14941 attgccgaga ttaaaaccac actttccacc ccgcttgttg ccgggcaacc gaaacaggat
    15001 gtcaccgacg tgctgtatac cgcttttatt ctgcgataac gacatgggcg atagtattct
    15061 ttctcaagct gaaattgatg cgctgttgaa tggtgacagc gaagtcaaag acgaaccgac
    15121 agccagtgtt agcggcgaaa gtgacattcg tccgtacgat ccgaataccc aacgacgggt
    15181 tgtgcgcgaa cgtttgcagg cgctggaaat cattaatgag cgctttgccc gccattttcg
    15241 tatggggctg ttcaacctgc tgcgccgtag cccggacatt accgtcgggg ccatccgcat
    15301 tcagccgtac catgaatttg cccgcaactt gccggtgccg accaacctga accttatcca
    15361 ccttaaaccg ctgcgcggca cagggctggt ggtgttctca ccgagtctgg tgtttatcgc
    15421 cgtggataac ctgtttggcg gcgatggacg cttcccgacc aaagtggaag gtcgcgagtt
    15481 tacccataca gaacagcgcg tcatcaaccg catgttaaag ctggcgcttg aaggctatag
    15541 cgacgcctgg aaggcgatta atccgctgga agttgagtac gtacgttcgg aaatgcaggt
    15601 gaaatttacc aatatcacca cctcaccgaa cgacatcgtg gttaacacgc cgttccatgt
    15661 ggagattggc aacctgaccg gcgaatttaa tatctgtctg ccattcagca tgatcgagcc
    15721 gctacgggaa ttgttggtta acccgccgct ggaaaactcg cgtaatgaag atcagaactg
    15781 gcgcgataac ctggtgcgcc aggtgcagca ttcacagctg gagctggtcg ccaactttgc
    15841 cgatatatcg ctacgcctgt cgcagatttt aaaactgaaa cccggtgacg tcctgccgat
    15901 agaaaaaccc gaccgcatca tcgcccatgt tgacggtgtc ccggtgctga ccagtcagta
    15961 cggcaccctc aacggtcagt atgcgttacg gatagaacat ttgattaacc cgattttaaa
    16021 ttctctgaac gaggaacagc ccaaatgagt gacatgaata atccggccga tgacaacaac
    16081 ggcgcaatgg acgatctgtg ggctgaagcg ttgagcgaac aaaaatcaac cagcggcaaa
    16141 agcgctgccg acgcggtgtt ccagcaattt ggcggtggtg atgtcagcgg aacgttgcag
    16201 gatatcgacc tgattatgga tattccggtc aagctgaccg tcgagctggg ccgcacacga
    16261 atgaccatca aagagttatt gcgtctgacg caagggtccg tcgtggcgct ggacggcctg
    16321 gcgggcgaac cgctggatat tctgatcaac ggttatttaa ttgctcaggg cgaagttgtc
    16381 gtagtcgctg ataaatatgg cgtgcggatc accgatatca ttactccgtc tgagcgaatg
    16441 cgtcgcctga gccgttagtg atgaataacc atgctactgt gcaatcttcc gcgccggttt
    16501 ctactgcgcc actgctgcag gtgagcggcg cactcatcgc cattattgcc ctgatcctcg
    16561 ctgctgcctg gctggtaaaa cggctgggat ttgccccgaa acgcactggc gttaacggtc
    16621 tgaaaattag cgccagtgct tcgctgggcg cgcgtgaacg ggtcgtggtg gtcgatgtgg
    16681 aagatgcacg gctggtgctc ggcgttaccg cagggcaaat caatctgctg cataaacttc
    16741 ccccttcagc accaacggaa gagataccgc agaccgattt tcagtcggtc atgaaaaact
    16801 tgcttaagcg tagcgggaga tcctgatgcg tcgtttgttg tctgtcgcac ctgtccttct
    16861 ctggctggtt acacccctcg ccttcgcgca actgacgggt attaccagcc agccgctgcc
    16921 tggcggtgga caaagctggt cgctcccggt gcagacgctg gtgttcatta cctcgttgac
    16981 gtttattccg gcaattttac tgatgatgac cagtttcacc cgcatcatca ttgtttttgg
    17041 tttattgcgt aacgcactgg gaacaccctc cgcgccacct aaccaggtat tgctggggct
    17101 ggcactgttt ttgacctttt ttattatgtc accggtgatc gataaaattt atgtcgatgc
    17161 gtaccagcca ttcagcgaag agaaaatctc aatgcaggag gcgctggaaa aaggggcgca
    17221 gccgctgcgt gagtttatgc tgcgtcagac ccgtgaggcg gatttaggat tgtttgccag
    17281 actggcaaat actggcccgt tgcagggacc tgaagccgtg ccgatgcgca ttttgctccc
    17341 ggcttacgtg accagcgagt tgaaaaccgc atttcagata ggcttcacga tcttcattcc
    17401 gtttttgatt atcgacctgg tgattgccag cgtgttgatg gcattgggga tgatgatggt
    17461 cccccccgcc accattgctc tgccctttaa actgatgctg tttgtactgg tggatggctg
    17521 gcaattgctg gtcggttcgc tggcgcagag cttttacagc tagagaggca aaatgacacc
    17581 tgaatcggtc atgatgatgg ggactgaagc gatgaaagtc gcgctggcac tggctgcccc
    17641 gctattgctg gtagcattgg tcacgggcct tatcatcagt attttgcagg ccgccacgca
    17701 gattaacgaa atgacgctgt cgtttattcc gaaaatcatc gccgtattta tcgccattat
    17761 tattgccgga ccgtggatgc tcaatctgtt gctggattac gtccgcacct tgttcactaa
    17821 cctgccgtat atcatcgggt aggccgtaca atgatgcagg tgacaagcga tcaatggctt
    17881 tcctggctaa gtctgtactt ctggccgtta ctgcgcgtgt tggcgctgat ctccaccgcg
    17941 ccgattctga gcgaacgcag cgtaccgaaa cgggtaaaac tgggtctggc aatgatgatc
    18001 acgttcgcta ttgccccatc attacctgcc aacgatgttc ctgttttttc gttctttgct
    18061 ctgtggctgg ccgtgcagca gatcctgatc ggcattgcgc ttggttttac catgcaattt
    18121 gcctttgccg cggtgcgaac cgctggtgaa attatcggtc tgcaaatggg gctgtcattt
    18181 gcgacgtttg tcgatccggc cagccatctt aatatgcccg ttttagcgcg tatcatggat
    18241 atgctggcgt tactgctgtt cctgacattt aacggtcatt tatggttgat ttcactgctg
    18301 gtcgatacct ttcacaccct gccaattggt ggcgaaccgt tgaacagcaa tgcgtttctg
    18361 gcactcacca aagcagggag tttgattttc cttaacgggc tgatgctggc gttaccgctc
    18421 attactctgc tgctgaccct gaatctggca ttaggtttac taaatcgtat ggccccgcaa
    18481 ttatccattt ttgttattgg atttccatta actctgactg tcggtatctc tttaatggcg
    18541 gcattaatgc cgttaattgc gcctttttgc gaacatttat tcagtgagat gtttaatttg
    18601 ctggctgata ttattagtga attgccatta atataattcc gtaacgttta tcatgctacc
    18661 ctaaggatta tccgaaaaat aatacctacg aacatcttcc aggatactcc tgcggcgaaa
    18721 tatttgtttt aagctcactc acatatcgca acatttactt tactttaaga caattccagg
    18781 caaattatac aacactttac gggatagtaa gtccgcctga aaaatcgcga gagtggcgca
    18841 ttaggtgacc catgttgttc cgtttagtca tgatgaaata ttcaggtaag gggaattatc
    18901 gttacgcatt gagtgagggt atgccatgtc aacgattatt atggatttat gtagttacac
    18961 ccgactaggt ttaaccgggt atctgttgag tagaggggtt aaaaaaagag aaatcaacga
    19021 cattgaaacc gttgatgacc ttgccatagc ttgtgattca cagcgccctt cagtggtgtt
    19081 tattaatgag gactgtttca tccacgatgc ttctaacagt cagcgcatca agcacatcat
    19141 taatcaacat cccaatacgt tatttatcgt ttttatggca attgccaatg ttcattttga
    19201 tgaatatcta ttggtcagaa aaaatttatt gatcagttct aaatcgatta aaccggaatc
    19261 tctcgatgat atccttggcg atattctgaa aaaagagaca acgataacct cgtttttaaa
    19321 tatgccgacg ttatcattga gccgaaccga atcgagtatg ttgcgaatgt ggatggcagg
    19381 tcagggaacc attcaaatct ccgaccaaat gaatatcaaa gccaagaccg tttcatcgca
    19441 taaaggtaat attaaacgta agatcaaaac gcataataaa caggttatct accatgtcgt
    19501 ccgactgacg gataatgtga ctaatggtat ttttgtcaac atgcgctaac acattctgac
    19561 tggtggtttc ccaccagtca ggctgaatta gattactctg ctttctccac aaagataccg
    19621 tcctgatgcc ctgcttcatt aaagaaccag atgccgagcg ggtagtcttc cagcgaaacc
    19681 aggtacattg tgccttcact aaactcctca actgccagta ccacgccagg acgacgcgga
    19741 ccgccatccg ttttgactgt tacccgatca ttcaccttca tcgttttcct cctgtggctt
    19801 tgtgccagtg tagaacaatt tcgtttttct ggcagcgcca ggcgcgcgcg agtgctgatt
    19861 ttctcgacgg tctatactta agagatgcca ggcggactta acgactggcg gcaacaacag
    19921 agtaacggtt gcgaggaaag atgatgaaaa ccgcaaaaga gtacagcgat accgcaaaac
    19981 gtgaggtcag cgtcgatgtc gatgccctgc tggcggcgat caatgaaatt agcgaaagcg
    20041 aagttcatcg cagccagaac gattctgaac acgttagtgt cgatggacgt gaatatcata
    20101 catggcgtga attggcggat gccttcgaac tggatattca tgacttcagc gtctctgaag
    20161 tgaatcgttg aatgcacaat aaaaaaaatc ccgaccctga gggggtcggg atgaaacttg
    20221 cttaagcaag aagcacttaa aaaattcgtt acaccaggaa atctgatgtg ttcatcacct
    20281 tatccgcaat ttttttcgct gacaagaaaa tattcgtgat atgaatgatt aacgctactt
    20341 atgaatagaa atgtgacgcg cgtcacccct tggtggctct ggcgtcaacg ggatcgctcc
    20401 caaaaaaatc atctttgata atttgtgctg ttttgagaat catcctggta aggggagtac
    20461 tgcgcaacca tgctttcaat tcaacaacca ctactggttt ttagcgatct tgatggcacc
    20521 ctgctggaca gtcatagtta tgactggcaa ccggcagccc cctggctcag ccgtttacgc
    20581 gaagcgaatg ttcccgtcat tctctgtagc agtaaaactt cagcggaaat gctgtacttg
    20641 caaaaaatgt tggggctaca aggcttaccg ctgattgcag agaatggcgc agtgatccag
    20701 cttgctaagc aatggcagga gatagacggt tttccacgca tcatctcagg tattagccat
    20761 ggcgaaatca gccaggtttt aaatacgcta cgcgagaaag aacattttaa attcacgact
    20821 tttgatgatg tcgacgatgc aaccatcgcc gaatggacgg gattaagccg tagccaggcg
    20881 gcgctgacgc agctgcatga ggcgtcggta acgctaatct ggcgcgacag tgacgagcgt
    20941 atggcacaat ttaccgctcg tctgaacgaa ctgggcttgc agtttatgca gggtgcgcgc
    21001 ttctggcacg tcctggatgc ctctgccgga aaagatcagg ctgccaactg gattatcgcg
    21061 acctatcaac aattgtcagg caaacgccca accacacttg gcctgggcga tgggccaaac
    21121 gatgcgccct tactggaggt aatggattac gcggtgattg tgaaagggct aaaccgtgaa
    21181 ggggtccatc tgcatgatga ggatccggcc cgcgtctggc gaacgcagcg tgaaggaccg
    21241 gaaggctggc gtgaagggct ggaccatttt ttctccgccc gttaagcgtt atcgctcgcg
    21301 aatacccgat tgcgcccggc ctgttttgcg aggtaaagcc gacggtcggc caatgactgg
    21361 agttgttcaa aatcataatc accggtttcc tcgctgctgc ttacccccag cgaggcactg
    21421 atgcgtatcg tcgtactctt ggcgatcagc atctcttttt catttaactt caggcgaata
    21481 cgttctgcga cctccgcagc ctgcgtcaga ttcgcgcctg gcagaatcac acaaaactcc
    21541 tcaccaccga cccgcccggc aacgtcctgc gcacgtaagg aactgctaat taatccggca
    21601 gcatgagaaa gaacacggtc gcccacctga tgaccaaagc ggtcattaat cgctttaaaa
    21661 tggtcaagat cgacctgaat gacagaaaat ggatgttggt gcgtctgaca caatttagcg
    21721 agcggacggg ctttttcgaa cagtgcgcca cggttatata aacgcgttaa ggtgtcgtgc
    21781 cacgcctgcc actgcaacga gctttgcaga acatacatgt tgctgaccat ccggcgaatc
    21841 acataccagg agagtagtaa catggtggta aagagcgccc acagcagggt taatgcaatg
    21901 ctgatactgc cgaaatcacc acgcacgcct tcgcttagcg tatggacgcg caccagcaca
    21961 ccgtcgaaat ggtccagacg ttcccagcta acataacgac tgttcatacg aatgccaccc
    22021 cgcgtgtcat gttccattgc ctgcgccaac aaggccagtt cacgaggatc aaaaatattc
    22081 cctgttggat ggtcaggatt ggaagaggtc aaaaatctca gcttactgtc atagagctga
    22141 tactcaccat cgaggttttt atcgatggcg tttcttaaaa attgctgcat ggtacgcacg
    22201 ggaatactca tccccagcac gccataccag tagttttcac tgtctaccgg aacactgacg
    22261 gtaacctgcg gttcagtatt gctggcgtgt tccggttgcg aagtgaacca gcgtaccgcg
    22321 cggtgacgat tttctcgttg cgaatggccg ataaaccagg gttgggtgac atagccgtaa
    22381 taacgcgttg gtacattgcg cgtaaacaag gtcggctgcg tcgaaacgta aaatccggca
    22441 cgcgagacat acatcgcctg ttcaaccatc gacgaggagt tgtgcgccag tcgcagcaag
    22501 taaccaactt ccagtgcagc ggtaatttca ttgtcgaggc tttcattttc gcgagacagg
    22561 agattcccct cgctgactaa tgcatccgac acgccattga ctggcagggt gcgtcgtcgg
    22621 ttgagttcga tttgccaggc gtgctcgtcg cgatgctgtt cgaactcggt tacggcatta
    22681 cgcagagagg tgaaatccag tggcgctacg agagcttcgc gcatgccatt acgcaaaaag
    22741 atcagtttgt cgacgttata ctgcaaatgc ttatcgagcg cgttggctac gttttccaga
    22801 tgattacgct ggctggagat ataggcatct tccagcacca ccacttcacg ccaggtgagc
    22861 aaggtggaaa aaagcaatac cacgataaag cagagattaa cgacatgacc aggccccagg
    22921 cggcgtgcga gttttttcaa ccagctctgg ttttccattt ttgtctcgtg ctgcaccctg
    22981 acgactccca acctgctttt ttatgattct ggcataaccc ggcgataacg taccgcaaaa
    23041 cagcgataat tttataatgg tggcatgata agacgcgact atcattcatt gcaggcattg
    23101 gcacaccagg ccggatgtga tccggcctgg tgttaatgat accctttcaa acttcttccg
    23161 cagaacgact cccctccccc ggaacaagat cggtttcatg aaaagcttcc cgcttgaccc
    23221 cgtaaccgtc ataccaacga cactcaacca taccgctgga gtatccagtg acaatcatcc
    23281 gcgggccgcc ctctttaacc gtaacttcct cactaaccat aaagctcata ctcgcctcct
    23341 tttttcgagt gaaacttgtt caccttagtt gaagatggcg aattttgcat aatagccagt
    23401 gcgagtatta atgtgcctga ccgcgcattt tcgctactgc tttcactccc agcaccacga
    23461 tgccgccgat gataaaacca agaatcagat ttaaaacagt cggtaatatc atcgccacca
    23521 ctgcactttg ctgcccggcg aaatgttcaa tggcgtgatg cagcggcgca ataccatgta
    23581 ccacaatccc gccgccgacg aggaacatcg ccagcgtgcc gacaatcgat aatgctttca
    23641 tcagccaggg cgcgataatc aataaccctt tacctaatgc ctgcatcagc gcgctggatt
    23701 tttccgccag ccaatacccc aggtcatcaa tcttaacgat aacccctacc agaccgtaga
    23761 cgcccacggt cactaccagc gcgatgcctg aaagcaccag cacctgatta agcaacggcg
    23821 cctctgccac aatccccagc gtgatggcaa cgatttccgc agacaaaata aaatcggtac
    23881 gaatcgcccc tttaattttg tccttttcaa acttcagcgg atcctgcgcc gccagcttct
    23941 ccagacgctg ctgactctgc gccggatctt ctttatgttt acgcgcctcc agcatatgca
    24001 gcactttctc taccccttca aagcagagaa acgcgccgcc aatcatcaac agtggcgtaa
    24061 tcgcccacgg gataaacgca ctgatgatca gcgccagcgg caccagaatc actttattga
    24121 tcagcgatcc tttcgccacg ccccagacca cgggaagttc ccggttggcc cgcacgcctg
    24181 aaacttgttg cgcattgagc gataagtcat cccctaatac accggcggtt ttcttcgccg
    24241 ccagtttgcc catcacggaa atatcgtcca gcagtgtggc gatatcatcg agcaacgtca
    24301 gtaaactact cccggccaaa agcattccct ctcattttta ttgttgaagg gtgtaagtat
    24361 gaagcaaaaa cggcagctct gatacttgct tcacaggtca acattttttt aacaatcaag
    24421 tgcaattata tctctcgcca gcagaatgat tttcacgttt actatgaccc acctttttat
    24481 ttcagctgtg agggattatg cgtttccgcc agttgttacc gctttttggc gcgctgtttg
    24541 cgttgtatat catttggggc tcaacctatt ttgtcattcg gattggcgtg gaaagctggc
    24601 cgccgttaat gatggcgggc gtgcgattcc tggcagccgg tattttattg ctggcatttt
    24661 tgctactgcg cggacacaaa cttcccccgc tacgtccgct gctcaatgcc gcgctgattg
    24721 gcctgttatt gctggctgtc ggtaatggca tggtgacggt tgccgaacat caaaatgttc
    24781 cttccggcat cgccgccgta gtggttgcaa ccgtgcccct ctttaccctg tgcttcagcc
    24841 gcctgtttgg cattaaaacg cgcaaactgg aatgggtggg tatcgccatt gggcttgccg
    24901 gaatcatcat gctcaatagc ggtggaaatt taagcggcaa tccgtggggc gcgattctga
    24961 ttttaatcgg ctcgattagc tgggcgtttg gctcggttta tggctcgcgc attaccttac
    25021 ctgtagggat gatggcgggt gcgattgaga tgcttgcggc aggcgtggtg ttaatgattg
    25081 cgtcgatgat tgcgggtgaa aaactgacgg cgctcccttc cctttcaggc ttccttgcgg
    25141 tcggctatct ggcgctgttt ggttcgatta tcgccattaa cgcttatatg tatttaatcc
    25201 gcaacgtcag tccggctctc gccaccagct acgcctacgt taacccggtg gtcgcggtct
    25261 tactgggtac gggactgggt ggcgaaacgc tgtcgaagat tgaatggctg gcgctcggcg
    25321 taattgtttt cgcggtggta ctggtcacgt tgggaaaata tctcttcccg gccaagcccg
    25381 ttgtcgcgcc agttattcag gatgcatcaa gcgagtaaat gaatcccctg cgtgtcgatc
    25441 tgcgcgctgg caccttcgcc gcagatccac tcttccagac gctcggtaag cgccgcatcc
    25501 gtaagcttct cgcgcccacg taacgcacac tcccagacaa tcagtacgcg ccagccgagt
    25561 tcctgcaagc gactgatatc gcggcgatcg cgttcaacat ttttacctat cttctccagc
    25621 cagaactcgg ttcgagtcgc aggcacttta aacagatagc agtgatgatg atgccagaag
    25681 cagccgtggg taaatatcac gcagcgatat tcatcaacga caaaatccgg gcgtccgggc
    25741 agactggcgt cctgaacgcg aaacgcaagg ccctgcccgg ttaacaaact ggcgaggcgc
    25801 ttctcaatcg ccgtatcacg cgtggcaatc gcgcgcatat ttttgctgcg ggtggcctta
    25861 tcgtgaacgt cgaccatgtt gtgcctcttg ctgacgcaac gccaccgcct gtttgatttt
    25921 tggctcaagc agttttgcca ccgcggcaaa gaccggcacg accaccgagt taccgaactg
    25981 gcgataggcc tgagtgtccg aaaccggaat gcggaatttc acttctcctg gcgcttcaaa
    26041 acccattaag cgcgcacatt cccgaggcgt taaccgacgt gggcgatgtt gctgattcag
    26101 cggatcgtca aagtcttttt cacccgtggc catatcccag ccgcgatcga ttaaaatttc
    26161 cgcgccatct ttgtaataac gagcagacag cgtacgcgtg acgctttgcg gattgttcgg
    26221 ataaaccatt ccataaccga agccattacc gcgcgcctga tgttttttcg cataacgata
    26281 gaggtacttc cacagcaccg gcgtcaggat atatttcgcc tcgaccatcg ggtccagcag
    26341 ctgggccagc gtcactcgct gcgcagggaa acattcgctg atatcacgca gggtaaaatc
    26401 ggctttcaga ttcagatcgc gacgaaaacc caccagcacg atgcgttcac ggtgctgcgg
    26461 caggaaatgt ttgccatcga tgattttcgg atcgtccggc ccgttatctt ctgcatcagc
    26521 cacgtcatag cccagttcgt ccagcgtctg catgatgatg cggaacgttt taccctggtc
    26581 gtgacttttc aggtttttga cgttttcgag cacaaacatc gccggacgac gcgcgtcgat
    26641 aatgcgcacc acatcaaaga acagcgtgcc ctgagtatcg caggcaaaac cgtgcgcccg
    26701 cccgagcgag ttctttttcg atacgccagc cagcgaaaat ggctgacaag gaaaacccgc
    26761 cagtaataca tcgtgttcag gaatgtgttg acgaatatgt tccgccgccg cctcatcact
    26821 cacgccttct ttatggctga gggtgatgtc gcggatatct tcattaaaat gatgcgtcgc
    26881 cgggtcgcaa taatgattgg ctttataagt gcgtaccgca tgtttgttcc attcgctggt
    26941 aaacacgcac tgtccgccaa tcgattcaaa accgcgacgg atgccgccaa ttccggcaaa
    27001 tagatcgata aagcgaaacg cataatgcgg atgatgtgcc ggtggtttgg gtaacaacgt
    27061 ttgcagatgg gcgaactctt tctcacttaa acggtgccat gccgagtcat tcgccagcgc
    27121 acgttttaaa attgccgcgc tccagtgatt ctcacccaca ccattaagct gcgccaccag
    27181 cgttttaaca tcataaattt gcagcagttt ctccagcatt gcctgaacga cattcccggt
    27241 gctgtatgaa tcggttactg atatattttc ctgcatagat ttaaccggcc atctgagatg
    27301 gccgacaggt taacataatt acagccattt agggccacgc gctcagcccg cgggtgaaaa
    27361 cgcatctatc accttgtgat cgacaccttc attctccccc gccagttcgg cactgagctt
    27421 cgccataaac tcgactaaaa attgcgcgtt gtgctgcgcc agctgcttgc ctcttgcggt
    27481 ttgcatggtt tgcggtagct tcagcaactt agtctggaaa tgatccagcg cgtagcgttt
    27541 atcatccagc gggcgatgct gtgcgaacgg atcttcacca tcaaacagcg ccacgcccaa
    27601 tgcccctgaa acagcaaaca cgcgcgccag gccaatcgcc cccagggctt ccagccgatc
    27661 cgcatcctgg acgattttag cttccgttgt taagggggcg atttgcgcgc tgaaactgtg
    27721 agcggcaatg gcatgacaaa cggcctcgat tttctccgcc ggaaattgca cgaactcttc
    27781 acggagcagg cgacgcgcct cttctgccgc caggattgaa gaatgctgcc gttgcggatg
    27841 atttttcgcc aggctcacaa tatcgtgaaa ataacaggcg gttaaaatca ccagcatatc
    27901 gacatcgtca tctgccgcca gcttctgtgc cgtagcccag acgcggcgaa aatgacatac
    27961 gtcatgggcc gcgtcctggt gctgatgatg gtttttcaac cagttttcga actgtgcctg
    28021 ccagtgttgt aagtccatac gcgctccgtt gccaaagagt cgcaacctta gcagttttta
    28081 ttcatagcgt ctgctacgtg cgggacgagg tcgggtgtac caggcaatgc tgcctaacaa
    28141 tgccccgacc agagccataa ataaaaatcc gaccagtgcc cctaaccact ttcccgcggt
    28201 agagcctaaa tcgctttcaa atcccgacac cggggcatca gggatccggc tcatcaccca
    28261 gatgtaacgc gccattgccc agacgaaata gccacaccat gtatagaaag cccacaaagc
    28321 caatttgccg ccagggctgc gagagagtct ctcttccatc tttgtcgaac cgtattccat
    28381 tttgagataa cccggaaaat tcagacttag tactattatg tgataaaagt cacatttttc
    28441 cacattggat gagccagtca agactcaatc attttttgaa caaatagata atttttgcgc
    28501 aacggtagtg aatacaggaa aaatcgtttt gagc
//
LOCUS       DAZKGF010000047        28164 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZKGF010000047 DAZKGF010000000
VERSION     DAZKGF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.047"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(37..1434)
                     /gene="ccp"
                     /locus_tag="ACHOQV_001973"
     CDS             complement(37..1434)
                     /gene="ccp"
                     /locus_tag="ACHOQV_001973"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFP9630349.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1839..3488
                     /locus_tag="ACHOQV_001974"
     CDS             1839..3488
                     /locus_tag="ACHOQV_001974"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP9630350.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKACARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3539..4141)
                     /locus_tag="ACHOQV_001975"
     CDS             complement(3539..4141)
                     /locus_tag="ACHOQV_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9630351.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4661..5560
                     /locus_tag="ACHOQV_001976"
     CDS             4661..5560
                     /locus_tag="ACHOQV_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9630352.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARSLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSRENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5609..6622
                     /gene="yhjD"
                     /locus_tag="ACHOQV_001977"
     CDS             5609..6622
                     /gene="yhjD"
                     /locus_tag="ACHOQV_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFP9630353.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7015..8337
                     /locus_tag="ACHOQV_001978"
     CDS             7015..8337
                     /locus_tag="ACHOQV_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9630354.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8516..10576)
                     /locus_tag="ACHOQV_001979"
     CDS             complement(8516..10576)
                     /locus_tag="ACHOQV_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP9630355.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLASDDNKDTNAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10646..11413)
                     /gene="pdeH"
                     /locus_tag="ACHOQV_001980"
     CDS             complement(10646..11413)
                     /gene="pdeH"
                     /locus_tag="ACHOQV_001980"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFP9630356.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11645..12574
                     /gene="kdgK"
                     /locus_tag="ACHOQV_001981"
     CDS             11645..12574
                     /gene="kdgK"
                     /locus_tag="ACHOQV_001981"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFP9630357.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSQTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12670..14166)
                     /locus_tag="ACHOQV_001982"
     CDS             complement(12670..14166)
                     /locus_tag="ACHOQV_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9630358.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLEAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(14387..15673)
                     /gene="dctA"
                     /locus_tag="ACHOQV_001983"
     CDS             complement(14387..15673)
                     /gene="dctA"
                     /locus_tag="ACHOQV_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFP9630359.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15856..17844)
                     /gene="hmsP"
                     /locus_tag="ACHOQV_001984"
     CDS             complement(15856..17844)
                     /gene="hmsP"
                     /locus_tag="ACHOQV_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFP9630360.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17926..21399)
                     /gene="bcsC"
                     /locus_tag="ACHOQV_001985"
     CDS             complement(17926..21399)
                     /gene="bcsC"
                     /locus_tag="ACHOQV_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFP9630361.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTSDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPLAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPLDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDTWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21381..22487)
                     /gene="bcsZ"
                     /locus_tag="ACHOQV_001986"
     CDS             complement(21381..22487)
                     /gene="bcsZ"
                     /locus_tag="ACHOQV_001986"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFP9630362.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWALLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22494..24833)
                     /gene="bcsB"
                     /locus_tag="ACHOQV_001987"
     CDS             complement(22494..24833)
                     /gene="bcsB"
                     /locus_tag="ACHOQV_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFP9630363.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVVLLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24844..27462)
                     /gene="bcsA"
                     /locus_tag="ACHOQV_001988"
     CDS             complement(24844..27462)
                     /gene="bcsA"
                     /locus_tag="ACHOQV_001988"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFP9630364.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27459..>28164)
                     /gene="bcsQ"
                     /locus_tag="ACHOQV_001989"
     CDS             complement(27459..>28164)
                     /gene="bcsQ"
                     /locus_tag="ACHOQV_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFP9630365.1"
                     /translation="TITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWAR
                     AMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICTGLQQLKASGRY
                     QWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIG
                     SQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANW
                     CLLNYSGLKTPVGSAS"
BASE COUNT         6611 a         7782 c         7107 g         6664 t
ORIGIN      
        1 atcgcccaaa cactcctgtt cgggcgatca aattaattat tgtttatcct gcatatacgg
       61 cgtgtaaacc ccgttcagac tgtgcaggaa agctacgata tcatccacat cctcctgcgg
      121 cagctctttg cctacctgat agcgcagcat cagtttcacc gccccgtcca gcgtcggcac
      181 gtcaccgcga tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg gtactttctg
      241 acgcaattta tcacgctctt ctttagtaac attcatacga ccaatatccg ccgccgtaat
      301 ttcaccaaag ttaaagtctt ttttcagccc caacggttca aaggaacgtc cgccgagaat
      361 aataccacca tgacaagttg cacatttatt atctttaaat aattgatagc cttttttctg
      421 ttgcgccgtc agagcattct catctccacg caaccattta tcaaatgggg aatccggcgt
      481 aattaatgtt ttctcaaatt cagcaatggc atcagtaata ttttcgccac tgaaaccttg
      541 tggatagact tcgaggaact gcgctttaag ctgcggatct ttttccagct tagcaataat
      601 ttcgtcccag gatttcgacg ccatttcaat cgggttcaac ggcggtccac cagcctgatc
      661 ctgcaatgtt gccgcacgac catcccagaa ctgctcaacg ttaaatactg agttaaatac
      721 cgtcggcgcg ttaatcggcc caactgcgcc accaacacca atcgatgttt ttctgccatc
      781 gacgcccccc gcattcaacg catggcaatg agcgcatgaa atggtgctat cagccgataa
      841 acggggatcg tgatacagcg caaaacccaa cgccactttt tgcgcatcgg taggcagttt
      901 ttgcgggatg ggctgcaccg gttcattgcg atgctccgga gcagtatcat tgctggcgta
      961 atattccgcg cgctgttttg caatccaggc cagtatttcc gcccgctctt catcactcac
     1021 cttacccgcc cagtgtagcg cggtataacg cgttggcggc atagtttcat actgcatcac
     1081 ccattcaatc ttattcagat cgctttgcga aacgggttta ttagccagca gagccgcacg
     1141 cacggcctcg aggttaaaag atttatatcc aagctttatg tcgtaatcca tcaactgttt
     1201 cgcgccggga atataataat aggcgggtaa ttctgccgaa ggtgtatggc aatagtcgca
     1261 tcctttttca cggagaaagc ctaaaacctt attattttca ctgacagcag atgcctgaac
     1321 atcggcctgt ttactgcgtt tattatcgtg gtaccacaca taaccagata accctaaata
     1381 gcaaatcgcg acgccagcca ggccgatcgc ggtaatacgt gagaccattt tcattattct
     1441 tccctcacgg tggtgagtta ttgtgaccca gaaaattttc cagaatgctg caaacgtctc
     1501 tgcgagacaa agccatcata aaaaacaggg agatatgctt tttgatagga atcaatcaat
     1561 tacatgaata taaactatca ataagatagc cagtgcctat tacccagatc aggaatggca
     1621 tatcatgatt gcggcgagag caggattgaa tgttctgaaa atgaaaaaca acacgaaatc
     1681 cgtattttta taaattccat tgatattagt gcgttatatt ttttggcagg ttaataaacg
     1741 tgatccaccg cacgctttgt cgcccaccag gcggagcgaa tgactaccct taaagaaaag
     1801 cccgataatt agcgacgaat ttcggaggtc ggatccttat gctcaatcag aaaattcaaa
     1861 accctaatcc agacgaactg atgatcgagg tcgatctctg ctatgaactg gacccatatg
     1921 aattaaaact ggatgagatg atcgaggcag aaccggaacc cgagatgatt gaagggctgc
     1981 ccgcctctga tgcgctgacg cctgccgatc gctatctcga actgttcgaa catgttcagt
     2041 cggcgaaaat tttccccgac agtaaaacct ttcccgactg cgcacctaaa atggacccgc
     2101 tggatatctt aatccgctac cgtaaagtgc gccgtcatcg tgattttgac ttgcgcaagt
     2161 ttgttgaaaa ccacttctgg ctgccggagg tctactccag cgagtatgta tcggacccgc
     2221 aaaattccct gaaagagcat atcgaccagc tgtggccggt gctaacccgc gaaccgcagg
     2281 atcacattcc gtggtcttct ctgctggcgc tgccgcagtc atatattgtc ccgggcggcc
     2341 gttttagcga aacctactat tgggattcct atttcaccat gctggggctg gcggaaagtg
     2401 gtcgggaaga tttgctgaaa tgcatggccg ataacttcgc ctggatgatc gaaaactacg
     2461 gtcacatccc caacggcaac cgcacctatt atttgagccg ctcgcaacca ccggtttttg
     2521 cgctgatggt ggagttgttt gaagaagatg gtgtacgcgg tgcgcgccgc tatctcgacc
     2581 acctgaaaat ggaatatgcc ttctggatgg acggtgcaga atcgttgatc cctaatcagg
     2641 cctatcgcca tgttgtgcgg atgccggacg gatcgctgct caaccgttac tgggacgatc
     2701 gcgacacacc gcgtgacgaa tcctggcttg aggacgttga aaccgcgaaa cattctggtc
     2761 gcccgcccaa cgaggtgtac cgcgatttac gcgcgggggc ggcctccggt tgggattact
     2821 cttcccgctg gctgcgtgat actggtcgtc tggcgagcat tcgtaccacc cagttcatcc
     2881 ccatcgatct gaatgccttc ctgtttaaac tggagagcgc catcgccaac atctcggcgc
     2941 tgaaaggcga gaaagagaca gaagcactgt tccgccagaa ggcctgtgcc cgtcgcgatg
     3001 cggtaaaccg ttacctctgg gatgatgaaa acggtatcta ccgcgattac gactggcgac
     3061 gcgagcaact ggcgctgttt tccgctgccg ccattgtgcc gctctatgtc ggtatggcga
     3121 accatgaaca ggccgatcgt ctggcaaacg ccgtgcgcag ccgattactg acacctggcg
     3181 ggattctggc aagcgagtac gaaaccggtg aacagtggga taaacccaac ggctgggcac
     3241 cgttacaatg gatggcgatt cagggattta aaatgtatgg cgatgacctt ctgggtgatg
     3301 aaatcgcgcg cagctggctg aagacggtga atcagttcta tctggaacag cacaaactga
     3361 tcgagaaata ccatattgcc gatggtgttc cccgcgaagg cggcggtggc gagtatccgt
     3421 tgcaggatgg gtttggctgg actaacggtg tggtgcgccg tttaattggt ttgtacggtg
     3481 aaccataata tttttacagc cagccgctaa cttcctgctg gctgtaaaat tatcctcttc
     3541 aggaggagat atttaacatc attgccgcct gggtgcgatt tttcacttcc agacggcgat
     3601 acagggattc cagatgcgct tttaccgttc cggtactgat attcagcgcc ctgccgatct
     3661 ccttatttga ttcgcccgcc gctaacatgg ttaaaatctc ccgctggcgg gcgcttaacg
     3721 atttgagatc tttaatgtcc ttttccggcg tcgtccgcca gtctccaggc agaaacatca
     3781 tccccatcgc cgcactattt accgccaacg caaatgtctc gacggttgaa tcacgaggca
     3841 caatggccag cacattaaaa tggataactt cctgtaacca ccgtttattg caatccgtcg
     3901 ccgtaattaa caccttaacc tcaggaaatt gcaccacggt tttttgcagc aaccagtaac
     3961 aaaactcacc atcctgatcg ccatcgagca tcactaaggc ttcagggtaa ctttccagct
     4021 tttgccataa ctcgtctgcc tgactggccc cctgaatact cactcctgga atacgttgct
     4081 gtaaactgat tttcattcca tgaataaata ttgactgcct gtcaaacatg actatttgca
     4141 taactgaatc tccacctgaa tacgttaaaa agacttaagt agtggaaggg tattacccgc
     4201 gagaaaaaat aagaattcgc catttggcgg tggccattct acagagatga cgcgtagaaa
     4261 atagttaccg atataaatag ttacagctaa acgcctgaaa ttacatgtcg agggcactat
     4321 ttaaaacaat tttgaggatt tccttatatt agtggttagt acgcatgcaa ttaaaaatga
     4381 aattccgcga ccacaagcca aaataacaaa tggcaatgag acaaaaataa gcacaaatag
     4441 ccaatacgtc ctctattcac tttaaaggga atcgctgaaa aatacgctct gtttaagggg
     4501 attcaccttt ctaagaaagc tattcctccc ttttcctact gagaaatcgc cacattcgtc
     4561 atgacaacat tgtgaaaccc ggctttagat gttagaaaaa acaataaaca atgcgatatg
     4621 cgcgttactg tttcgctgtg aacaacaatc ggtcaaagaa atggataaaa ttcacgcaat
     4681 gcagttgttc atcaaagtcg cggagctgga aagtttttcc cgcgcggcgg atttctttgc
     4741 tttgccaaag ggaagtgttt cgcgccagat acaggcactg gaacatcaac ttggcaccca
     4801 gcttctccag cgcactacgc gacgggtcaa actcacgcca gaaggcatga cctattatca
     4861 acgggcaaaa gatgtgttga gtaatctcag cgaactggac ggtctgtttc aacaggatgc
     4921 caccagtatc agcggtaaat tacgcatcga catcccgcca ggaatcgcga aaagcctgtt
     4981 actgccgcgc ttgtcggaat ttctctatct gcatccggga attgagctgg aactgagtag
     5041 ccatgaccgt ccggtagata ttcttcatga tggttttgat tgcgtgatac gcactggcgc
     5101 attaccggaa gatggcgtta tcgcccgttc tctcggcaaa ctgaccatgg tcaactgtgc
     5161 cagtccacac tatctgacgc gctttggtta tccgcaaagc cccgatgatc tgacttcaca
     5221 cgcaatagtg cgttatacac cgcacctggg tgtacatccg ttaggttttg aggttgccag
     5281 cgttaatggc gtccagtggt ttaagtctgg cggcatgttg acggtaaaca gtcgcgaaaa
     5341 ctatctcgcc gccggtcttg ccggtctggg gattattcag atcccgcgca ttgccgtgcg
     5401 cgaagccctg cgtgccgggc ggcttattga agtgttgcct ggctaccgtg ccgagccgct
     5461 ctccctttca ctggtttatc cgcagcgtcg ggagctttcc cggcgtgtaa acctgtttat
     5521 gcagtggctg gctggcgtaa tgaaagagca cctggactga ccgactatac tttttaagaa
     5581 tgaccacgac agaaggacaa aagagcggat gacgcaggaa aacgagatca aacgtcccac
     5641 ccaggatctg gagcacgagc cgattaagca gttggataat agcgagaaag gcggcaaagt
     5701 tagccaggcg ctggaaaccg tcaccactac tgccgagaaa gtccaacgcc aaccggttat
     5761 tgcgcacctg attcgcgcaa cagagcgctt taacgatcgg ctgggtaacc agtttggtgc
     5821 ggctatcacc tatttctcat ttttgtcgat gatcccgatt ttgatggtat cgtttgccgc
     5881 agggggcttt gtgctggcct cccatccgat gttgctacag gatatcttcg acaaaattct
     5941 gcaaaacatc agcgatccga cgctagccgc cacgttgaaa aacaccatca acaccgccgt
     6001 tcagcagcgt actaccgtag ggcttgtcgg cctggcggtg gcgctttatt ccggcatcaa
     6061 ctggatgggt aacctgcgtg aagcgattcg cgcccagtca cgcgatgtct gggaacgctc
     6121 gccgcaagat caggagaagt tctgggtaaa atatctgcgt gattttattt cgctgattgg
     6181 tttgttgatt gcgctgattg tgacgctttc gatcacctcg gttgccggtt cggcgcagca
     6241 aatgattatt agcgccctgc acctgaacag cattgagtgg ctgaaaccga cgtggcggtt
     6301 gattggcctg gcgatttcca tcttcgccaa ctatctgctt ttcttttgga tcttctggcg
     6361 cttgccgcgc caccgtccgc gcaaaaaagc cctgattcgc ggaacatttc tggctgcgat
     6421 tggttttgaa gtgattaaaa tcgtgatgac ctacaccctg ccatcgctga tgaaatcccc
     6481 ctctggcgca gcatttggtt ccgtgctggg actgatggca tttttctact tcttcgcccg
     6541 tttgacgctg ttttgcgcgg cgtggattgc caccgccgaa tataaagacg acccgcgaat
     6601 gccggggaaa acgcagcctt aaaataggcc ggatgcggcg tacgaattta ttcggcctgg
     6661 ctccccgtag gccggataag atgcgccagc atcgcatccg gctataatgc gcacataacc
     6721 tcttgaaact ctttcccaga gcctctttag ccatctattc aggagcaaac aatttcatac
     6781 caactcataa ccccagcata taaatccagt tggtaacttt tatttaacct gaaaccagtt
     6841 ttatccacta tttataaaat tatgtgaagc atttcataga agaaaaatca ctggcctaaa
     6901 cattatcccc tttttgcctg gtttttgacc atttccgcga tttgttacac attgaaatat
     6961 cacttttgct gtgcgtaata tggctattcg ttagccaaaa aataagaaaa gattatgcaa
     7021 gcaacagcca caacactcga ccacgagcaa gaatacacgc cgatcaactc gcgtaataaa
     7081 gtccttgtcg cctctctcat tggcacggcc attgagttct tcgactttta catttacgcc
     7141 actgcggccg ttattgtgtt tccgcatatc ttcttcccgc agggcgatcc tacggcagca
     7201 acgctacagt cgctcgccac ctttgccatc gccttcgtcg cgcgccccat tggctctgcc
     7261 gtttttggtc attttggcga tcgcgttggg cgtaaagcga cgctggtcgc ctcgttgcta
     7321 acgatgggga tttcgaccgt ggtgattggc ctgctgccag gctatgccac gattggtatt
     7381 ttcgccccgc tgctgctggc gctggctcga tttggtcagg gtctgggctt aggtggtgaa
     7441 tggggcggcg cggctctgct ggcgactgaa aacgccccac cgcgcaaacg tgcattgtac
     7501 ggctccttcc cgcagctggg cgcaccgatt ggcttcttct ttgccaacgg cactttcttg
     7561 ctgctttcct ggctactgac cgacgagcag tttatgagct ggggctggcg tgtgccattt
     7621 atcttctcgg cggtgctggt cattatcggc ctgtatgttc gcgtgtcgct gcatgagtcg
     7681 ccggtgtttg agaaagtcgc taaagcgaaa aaacaggtga agatcccgct gggtacgctg
     7741 ctgaccaaac atgttcgcgt aaccgtactg ggtacgttca ttatgctggc aacctatacg
     7801 ctgttttaca tcatgacggt ctactctatg acctttagta ccgccgccgc gcccgttggg
     7861 cttggcctgc cgcgtaacga agtgttgtgg atgctgatga tggcagttat tggttttggc
     7921 gtgatggtgc cagtcgccgg attactggct gatgcctttg gtcgccgtaa aagcatggta
     7981 atcatcacca cgctgatcat cctgttcgcg ctgttcgcct ttaacccgtt gctcggttct
     8041 ggcaacccga ttctggtttt tgccttcctg ctgctggggt taagtctgat ggggctgacc
     8101 ttcggtccga tgggcgcgct gttaccagag ctgttcccga cagaagtgcg ttacaccgga
     8161 gcatcgttct cttacaacgt agcgtcgatt ctcggggctt ccgttgcgcc gtatatcgca
     8221 gcctggttgc agactaacta cgggttaggt gcggtggggt tatatctggc ggcgatggct
     8281 ggcttgacgt taatcgccct gctgctgacc catgagacgc gacatcagtc gttgtaataa
     8341 gagtgaaaag cctgatgcgc tacgcttatc aggccagaat ttccatacaa tatattgaat
     8401 tagcaaggat ttgtaagccg gaggcgttca cgccgcatcc ggcatgaaca aagcacactt
     8461 tgccaacaat ctaaatctgt ttaattgccg gatgtcagac atccggcaat tatcatcact
     8521 tcttcatctg cgacaaaatt gtcctgcact gattagcctc cccttcggaa ggtgagatca
     8581 gcgccagtaa cgccgccgcc ggtgttacca gcgttgccag tgccgccgca acggctccgc
     8641 gagcaatcaa cggcccggct ttcaccccag cctgcggatt tttaaacgtc ccacgtacat
     8701 acagcggcga acgcaatgtg ataatccgaa tccctttact ctccggatca atagtcaaat
     8761 ccagctgttc cgaagcaaaa cttgccgtac cggtaacgtt aatcaacgcg ttctcagtat
     8821 cgaaagcaaa aatctgcggg cgcgccacgc cgttggcaat atccagattc gccgccgcac
     8881 agttcacccg cacctcatca tcaccaaata tcgcaccgac aatgtagtta ccgacattca
     8941 gcccgacaat ctccatcagg ttgcggctca ccagcccgtc attcatcagc agtttcaggt
     9001 tgccgttact gttgcctaaa agcgccgcca ccgagttacc gctaccgcgt agttccgcgt
     9061 caccgttcat ttcccccagc gtcttctgca tcagttctac atcgggcatc agttctttca
     9121 gtttcagtcg acgagcctga atatctgccc gcccttgcat cggtttttta tcaccttcca
     9181 gatgaatatt cgccgcaatg ctgccgcctg ccatgccaaa tttcagcggt tgcaggcgca
     9241 ggtcagcatt tttgaggatg atatgagtag aaagatcgct aatcggcagg ctactgccat
     9301 gctcaatgcg ccgccctttg aagcgaacat cggcatccat gacgtcccat ttatcggttt
     9361 cgaagcggtc ataaggcagc actttgcccg caggctgaac gcttttttca cccttcttct
     9421 gttcagaccg tttcgacttt tcagctcctt tcccggaatc aacgccaatt aacggcccta
     9481 aatctgccag ccgcaattgc cgcgactcaa catcaccttc cagttttggt cgtggcttgc
     9541 cggtggtgta gaccagagaa ccgtggatat cgctatcgcc aattcgccca ttaaaaccgc
     9601 gataatcaaa gaccgacgat ttttcagtgt cgattttcgc taccagccga ccatccgttt
     9661 caaacggcgg ggtatcgggc agcagaacgc ccgtcagttc atagagatca cccagcgaat
     9721 cgccagaaaa tttaagccgt aaatcgacac cacccatctt cattgggtca ttcacaacgc
     9781 catcaaaagc aacacgggtg ttaccagagc gaaaatcagc ctgtacagga aatggcgtcc
     9841 cttcgccacg cagcgccagc ataccgccta tttttcccgt accagtgagc ggctctccgt
     9901 tataacgtcc ctgcgccttc aggccaaaaa cgtaatcgcc caccttttct ttatccgctt
     9961 tacctttcga tccagtaact tcgctgaacg gcagcggctt gcctaacgga tcgacaaaaa
    10021 tttctagatc cgctttgctt actttgtcat caatggcgat ccgcccttga tcgaacagaa
    10081 tattatccag ccgaaacgac catgccgacg gctttgcatt cgtgtctttg ttgtcatcgc
    10141 tggcgagatt aaatgtccag ttgttgttct tttctgagag gcgaatcagg cgtgcgtcgg
    10201 gcttttcgag cttgatccac ggcaaccaga ccgttttggt cagcagcgcc agcggggcca
    10261 gcgtcgcttc tactcgcggc aaatgcacca ttgtgacttc gggaatatcc ggtggattgc
    10321 caagaatgat gtcttccgcg tgtacatggg gccacggcac ccagctgcgc cagccagttt
    10381 cttgtttttg ccgctcccac accacgccca gatcgccacg gatagcgaac ggacgattca
    10441 actccgcaga gactttctgg ttgatggtcg gtttgagtcg attccagtca aaagttgcaa
    10501 tcaaaatgat cgccacgaca atcaacaaca agaaagcccc tgaaatcgca gcggttattt
    10561 tgcctgcctt gctcatcgtt cgcttcctca tattcttcct gtgccagtcc taaagatagt
    10621 ccagccaggc ggaaaatgag gcagcttata gcgccagaac cgccgtattc agcgtttcta
    10681 tcggtgccgg gcgtgaaaga aaccagcctt gtgcggcgaa tgcgggcgag ttctgaacat
    10741 cacgccactc ttccggcgtt tctacgccct cgacaatcac cccgcgacaa tagcgattca
    10801 tgagatgtaa aagctgagaa aagagtgtgc ggccttccgg cgactgacgc agcatcacga
    10861 acagctctcg cgcgattttg atgtagtcat aacgcacttc acttagcgca gagaaatttg
    10921 ccatcccggt gccaaaatca tccagccaca gcgggccaaa ttcacacatc gaggcaaagg
    10981 ttgaatcttt cggcagacgg atatgctcca ccagttcgaa acgcagccag ggaagacgct
    11041 caatttggcg caggattttt ggttgctgac gcagggcgat gagcgtaggg ccatcaatat
    11101 tgaccgatgc cagcaggtcg tgctctataa agaagtcggc tttttgcgcc agcaaatcaa
    11161 tctgctcttt cacaacctcc atccgatggc taacggtgat ttcagtaaaa tagcgatccg
    11221 gcggcaggcg ttgcgaaggg ttcaagggat gcgtgaccac cgttaatagc tccacggcca
    11281 ttaaccgtcc gcatgtttga tagatcggct gccaggtgta agcacgctca cactgcaacc
    11341 aaaaacgccg ttcctgcaag ctctcgatgc ttgcttcagg gttgcttatt cgctggataa
    11401 cctgccttat catcaaagat gtcctgtgtt taaaagtgat gctgcccgga ctcgacaaag
    11461 attatcggcg cgcaagggca gaactttacc gcctggtcgg ggtaaatttc cccactccac
    11521 aacggaatct ttcaggaata cgttctggct caaaaaaata atggaacact gttttaatat
    11581 ggttgaccag caaaccacca cagcgcaaac taacgctaat tttttacaga tcaggttcac
    11641 gactatgtcc aaaaagattg ccgtgattgg cgaatgcatg attgagcttt ccgagaaagg
    11701 cgcggacgtt aagcgcggtt tcggcggcga taccctgaac acttccgtct atatcgcccg
    11761 tcaggtcgat cctgcggcat taaccgttca ttacgtaacg gcgctgggaa cggacagttt
    11821 tagccagcag atgctggacg cctggcacgg cgagaacgtt gatacttccc tgacccaacg
    11881 gatggaaaac cgtctgccgg gcctttacta cattgaaacc gacagcaccg gcgaacgtac
    11941 gttctactac tggcgtaacg aagccgccgc caaattctgg ctggagagtg agcaatccgc
    12001 ggcgatttgc gaagagctgg cgaatttcga ttatctctac ctgagcggga ttagcctggc
    12061 gatcttaagc cagaccagcc gcgaaaagct gctttccctg ctgcgcgaat gccgcgccaa
    12121 cggcggaaaa gtgattttcg acaataacta tcgtccgcgc ctgtgggcca gcaaagaaga
    12181 gacacagcag gtgtaccaac aaatgctgga atgcacggat atcgccttcc tgacgctgga
    12241 cgatgaagac gcgctgtggg gtcaacagcc ggtggaagac gtcattgcgc gcacccataa
    12301 cgcgggcgtg aaagaagtgg tggtgaaacg cggggcggat tcttgcctgg tgtccattgc
    12361 tggcgaaggg ttagtggatg ttccggcggt aaaactgccg aaagaaaaag tgatcgatac
    12421 caccgcagct ggcgactctt tcagtgccgg ttatctggcg gtacgtttga cgggcggcag
    12481 tgcggaagac gcagcgaaac gcgggcacct gaccgcaagt accgttattc agtatcgcgg
    12541 cgcgattatc ccgcgtgaag cgatgccagc gtaaaagcag catatgcaaa aggctggata
    12601 gagtgttacc cccctatcca gctcatccat aaatacctga tggttgtgct aactcatcac
    12661 catcaggcat tactgtacag gtggaatatc cgtcacttca ggatgtacgt catccgtggc
    12721 gacagatgtg gcggcagccg cagtcgatgg ggccatgatt tgatcccagg ccgcctgcaa
    12781 cgctttcatg ttaaattccg gctcgccttt cggctgcagc agtattaacg ccatatcatt
    12841 cgacaactgc tgacgcagat cctgatttaa catctccacc gtcaggctgt taaggaaatc
    12901 ctgacgcagt ttctgatatt gctccggcgc gatatcgacg acctgatttt gcaacgaacg
    12961 catccgctgc cccatcagaa tatcggtatc agctcgcgca taggcggcaa acagtttctg
    13021 cagctccagt ttcttttgcg ccactaacgc attgaactct tcttccggca gacctttatc
    13081 gcgaaccttc gccagttcac gcgccaccag attaaggttg ctgttcagct tgtcgtttgg
    13141 tgattcgatg ttgatggcac actgcgcacg cagatacagc acacgacagt caaatccaag
    13201 accgatgtct ttactgttac tggcgcttaa cgcttgctga acatgccaga acagcgcctc
    13261 acgggccagg tccgcacgcc agtagcgcag cagtgcggct gattcacgaa tcggctgcca
    13321 cggcgtatcc cacatgattg ataaccggtc ctgacgcacc gcgtcagtca taatactcac
    13381 cgcttccgca cgcagcggag aaagcgtcgg caccggagcc ggtgtttcac gtttgccttt
    13441 cagttcgcca aacgttttat tgatttggtc gacgaccgag cgcgcatcca cgtttcccac
    13501 caccagcagc gtcattgcat ccggggtata ccatttctga tagaaatctt taatcttttc
    13561 cgcttcaacg ggttgtttca gcggatcggc aggatcgtga cctaacaagg ttgatccttt
    13621 cagacgatag cgccaccagc cctctttagt atcggcaggc caggttgcca ccatgtcctg
    13681 actttgcagc gcgtggttga tggtttctgg tgtgatggtc agtttgccag tggcatttgc
    13741 cagataagag agcgcttctt tcagcaagtc gttacggtta ttgggcaaac tcagattaaa
    13801 cagcgtggtg tcataagaga caattactgg cggcatcggg cgtttagggt cgatcccctg
    13861 ctgccacaat gaacgcgcct gtgctgcttc aaggccaccg ctttgcgtta acgcaatacg
    13921 agggatggcg tggctgtaac cgctctgttg tgtactttcg gcgagcgaac cggtattaac
    13981 cagcaggcga atttcaacac gatcgctagg acgctggggc gtggtcagca cttgccactg
    14041 taaaccgttg gaaagcgtcc cctgttgcca tgctggatca ggctggagcg catctgcctg
    14101 cacatagcca gcagtggcca tcatcagcaa accgcccgct aaaagtcgaa tttttgtgcc
    14161 ctgcatgtga acccctgatc aactatcctg gtaataaaaa aactgcccgt tgtcggtcag
    14221 tctaaatgac gttaaaaaca cttcgtgtta gaccgcaaga acatgaaaat gtcacggaag
    14281 aagtgaaata aacctgaact cacccaggtg gtaagttcag gcacaggggt caattatgcg
    14341 caaacacccg cactcgggga agggagtgcg ggcataagtg atgagattaa gaggataatt
    14401 cgtgcgtttt gccatccggc gcacgattat tcagcacatc gtccagtttt ttgtggtcca
    14461 gttctttcac ccacttagca acgacaatgg tcgctacgcc gttaccgacc aggttagtca
    14521 gcgcacgagc ttctgacata aagcggtcga taccaaggat cagcgccaga cctgctaccg
    14581 gcaaatggcc caccgcagag agcgtcgccg ccagcacgat aaagccactg cccgttaccc
    14641 cggcagcccc tttggaagag agcaacaaca caattaatag cgtaatttgg tggacgatat
    14701 ccatctgact gttagtcgcc tgggcgataa acaccgccgc cattgtcagg tatatcgatg
    14761 tgccatcaag gttaaacgag tagcctgtcg ggatgaccag ccccaccacc gatttccggc
    14821 agccgagttt ctccatcttg tcgagcatac gcggcagcgc cgactcggaa gaagaagtgc
    14881 ccagtacaat cagcagctct tcacggatgt agcggataaa tttgaagata ctgaaaccag
    14941 tcgctttagc gattgaaccc aataccagca ccacaaacag gatacaggtg atgtagaaac
    15001 agataatcag ctgccccagt tgcaccagtg tgccgacgcc gtatttaccg atggtaaacg
    15061 ccattgcccc gaacgcacca ataggtgcca gacgcatgat catattgatg atgccgaaga
    15121 tgacctgcga gaaactttcg atgacgttaa aaatcagttg gcctttgctg cccagacggt
    15181 ggagcgcaaa accaaacagt acggcaaaca gcagcacctg cagaatgtta ccgctggcaa
    15241 atgcgccaat gacgctcgcc gggatgacat ccataatgaa ggcgacaatg ccctggtctt
    15301 tcgcctgatc ggcgtaaacc gctaccgctt tcgcatcaag cgttgccgga tcgacgttca
    15361 ttccggcacc aggctgcacg acgttaacga tgataagacc aataatcagc gcgatggtac
    15421 tgacaatttc aaagtaaagc agtgcgactg cgccggtacg accaaccgcc ttcatgcttt
    15481 ccatgcccgc aatgcccgtt acgacggtac aaaagatgac aggagcgatg atcatcttaa
    15541 tgagcttaac gaacccgtcg ccaagcggtt tcatttgctc gcctatttca gggtagaaat
    15601 ggccaaggag aataccaatg gctatcgctg tcaggacctg aaagtaaagg cttttaaaca
    15661 gagaggtttt catagggtgt cctttagtaa aaccacaggt cttgtaaggt tatggggtac
    15721 ctgcggccct aaaataacac ccagacaaca tcacagaaat gtacctggat cataattgaa
    15781 acaaaaaggt taaaaagttt gagctggctc gcacaaacca gcacttttta aagttttgta
    15841 atcagtttgg ggtagctact tttcttccag gtaactctct tcgaagattt caatagggag
    15901 tgggcgagca aaaaggaagc cctgggcaat accaacgccc gcttttgcca gccagtcgcg
    15961 ttgtgcttca gtctccacgc cttcggcaat catttgtaag ttcaggcttt gcgccaacat
    16021 gataattgca gcaatcatgc tgctatcttc cggcaagcct tcaacaaaca ttttgtcgat
    16081 tttcagtacg tcgattggca acgatttcat atgctgcagc tgacgcagcc ctgcgtagcc
    16141 catgccgaaa tcatccagcg ccacccgaac tccggcattg cgcagcggac ggaggattgc
    16201 caccgcagca tgagggtcgt caatacgtcg gctttctgtc acttccagaa tcagtgttcc
    16261 cggctgaatg cgatagcggg ttaacagttc cagcatatcc gctaccatat tcgggtgcat
    16321 cagttgtagc gctgagaggt ttaccgacaa gggcagcata atgccgcgct cttgccaggc
    16381 agcaagcagt cgacaagact cttccagcac ccagtgacca acggtaacca tcagcccaca
    16441 gcactcaatg cgatcgatta agccatccgg cagatcccaa ctgccatccg gttgctggat
    16501 acgcagtaac acttccgcac tgaccagttt accgctggtc atctcgacct gtggctgtaa
    16561 ccaaatcgca aattgatgat tttccagtgc attaaggata tcgctctctt ccgtcaaccg
    16621 ctgctgggcg gcttccatct gctgcggatc aaagaactga atctgatttt tgcctttatg
    16681 gcgagcggta aatgccgcag aaatagcgcg actgtaaagc tgttcggcgg tgagatcgcc
    16741 gtagaacatc gccacgccaa tgctacagtg cggacggagt tgaatacgtt caatcggcag
    16801 gcgctcgctc atgatagtga gcacttgctg acctaatgtg atagcgtgcc acggttcctg
    16861 tacaccgttg gcaatgacag caaagtcata accgctaatc tgcgcgagga tcatgcgtgg
    16921 cgacagtacc gatttgagtt tttccaccag cgtcagcagc agaatttctc gttgcgcctc
    16981 tttcagcacg cccgcagtat cacgcagggt ttcacaggtg atgatcatca gcgcggtggt
    17041 ttgtttacgc gcgacaacct gctccagcat ctccatcagc aaggctttgt tcggcaaatc
    17101 cgacaccggg aagcgcatcg cgttttcgtt ctgttcttca taatggcgct gcagcaattg
    17161 ctggttgagg ttgtaactgc gcaccagcat accgatttca tcgtcctgat gcagacgtgg
    17221 taatgccagt tggtgaccaa caagctcctg ggctgggatg gcgttaagtt cgcgagcaat
    17281 attgcgtaac ggatgtaaaa tcagtcgatt aatacaccag ctgatggcga cggtcaggat
    17341 aagcgacaaa agtaagtaaa tggtcactaa cgttgagagg gtgctcatca cgaacttata
    17401 catacggaag gaatccgcct gtagcaccag ataggcaatt ggctgcggat ttgccggacg
    17461 ttcgagcgag tagacgccca gcgagatttg aaccggtagc tcaaacaggc gagtaaccat
    17521 taccggcacc ggacgctctg gaataaaact tttacgcagc gcctggaact ggttgggcag
    17581 caccacatcg gcacggctga ccacgcctgc cggtttaatg ctggcaagaa tagcttccgc
    17641 ttcgggaata tcgcctttca aaatagctga agataagggt tcgcggacag agcgagcaat
    17701 gctttccagt tgcgtagccg tgttatagcg attctgctgg accagatgga acagcaaaac
    17761 ggtgcaaaaa ataaaaacga acaccaggac aacggctgcc accattgcca tctgcttgat
    17821 tgttaacgag cgacttacgc gcaaattgtc tccccccgga aatcccagat tgccgcccga
    17881 gtatacccga tcgcagcggc attaagagag gcgctatctg aaaacttacc agtcggcgta
    17941 aggtatcagc ggctgcggcg gtaaatccat gtcaccctgc catccggcgg cggaataacg
    18001 tacgtagagc aggaaatggc tgggtgcgta atctttcgcc tgctggatat caattgccgt
    18061 gccgacaaac cagttggaag taacacgtcg ttcaagtaat gcccgcgccg tatagccgaa
    18121 gccctgactg ctgccgccat cgttggattg ccgcgcagct tcttcctgcc agtcggtcgg
    18181 gatcagattc atcagcggat aacgcggcat ggttttggtg cgtgaatgcg accacgagcc
    18241 agacgccccc agctcccacg accagttttc cgtgcgctcc cgccacatca ccggtatggc
    18301 aaacgacagg tattcctgcg ggctgtagta accgccctga ccgagtgagt agccactcag
    18361 gtctttgtcg taatgccaga tcatgttgtt caggccgatt gtgacgcggc gattgttctg
    18421 gttaatgacc ttatagtaat agcccgtcat ccagcgcaca cgccagttat cttcgacatt
    18481 tttaccggtt aactggtcgc cactaagcga tgcccagacg ccgtttgctt cacctttatc
    18541 gtagctcagg cttagcccca cgccgtcggc acgcacgccg ccccattttt tcccggtatt
    18601 gctcggggag tctttttgcc caccaaaggc cagcaaagaa ctggagatgg gccgacggtg
    18661 ggcgttaacg gtgtaaccca gcggcccgat atcatcgctg taactgatgc cgccgaccac
    18721 atccaccacg ttgaagccca tcggcgtggt gccgatatcc cagctccagg tgtcgttgcg
    18781 ccaaccgacc gccacgctgg caccggaatc cgactggctg cggttgccgc tacagtcctg
    18841 taatgtacag gtgccccagt tgtcatccca tttaccatcg gcattagtgg agaaactgcc
    18901 gacgttcata ttgacgaaat cactgcgaaa gaacatccgc ccgtcagaat acggcgcatc
    18961 cacctgcaac atggtagtgt gcgctttcag atcggagtaa ccaccggtgc cgctcgaacc
    19021 ccagtaatca tgctcaaggg tgacgttaag atcctgctgg cgatagaggt ccgccgcatc
    19081 gctgcgcacg ccgcgtttca gccagtcatc tttctcgtca ttgcgggtca gtcgggtaaa
    19141 ggtgtcgtta tccagcggac gcgtcgtggt cacaccggat gcaaccatcg cgtctttgta
    19201 ggtttccagc gcctgcgttg gatcgcccgc ctgcgcttca aacttcgcac catcacgcag
    19261 taccatcgca ctttccatcg acggcggctg agattttgcc agcgggatca acttattaaa
    19321 cgtctgctgc gccgctgcgg tatcgccaag ctgcgcctgt gccagcgcca cgcgccgctg
    19381 agtgttcagc gaggcgttat cggtggcagg cagcttcgcc agctggctac gtgccgccgc
    19441 tttgtcaccg gcagcaatat ccacttccgt cagaccaaga atggcatcgg cgttagttgg
    19501 ctcccgcgtc aggacattct gatatgcagc gcgagcggcg gtgtaatcac gtcgttgttg
    19561 cgcccagtca gccagcgtga ggtctatgcg cgtggaaggt ggttgctggc gcagcatcgc
    19621 ttccgcttca gcttctttgc cgctttctcg caggcgatta gcggtttcca acacctgatc
    19681 gctttgcagt cgattaacca gctcctgaat attgctgctc cactgcgcgc gcggcaggct
    19741 gttgatatgc gccagcgccg ctctgtcctg gtcatgacca gagagataca gcccgtaagc
    19801 gtaaacctgc tccgggtcgt tcggcttctg ctgcgccaga ttacgcatta acgtatcggc
    19861 ctggctgcgt tgtccggcct gccagagatc ctgtgaaagt cggtaagtaa tccatacact
    19921 gccggggtcc agcgccagtc gttgccgctg aagtgctgcc gcctgcgccc atttcccctg
    19981 gttttccagt gcctctgcct gctgtgccag acggtcgttt tgcaggctgc gttcgatatc
    20041 atcaatgctg cgccgctggc tggcagagag cgaggcgata tacgcttcgg ctttttctgg
    20101 cgactgctgg cggtaaatat tcgccagccc gcgcacggcg ttagtgttgc cgctgtccat
    20161 acgcaaggtc tgctggtaat agcgttcggc ggcgggataa tctttgcgcg ccatcgccac
    20221 atcgcccagc ccaagcactg catagctgtc ggtgttatcg acattacgtg cctgctggaa
    20281 cagccgttct gcccggtcag gattattggc tttcagcgcg gcatcgccct gctggatcgc
    20341 cagccagtag cggtttactt tcagcagact gttccatttg tcgttattgc tgttgtgcgg
    20401 atccagcgcg agggcttttt ccagattcgc caccgcatta gcgcgatcgc ctttctgaga
    20461 atacgcctgg cccagcgccc ccagagcctc actgtctttc gggttcgccc gcaccgcctg
    20521 ttgtagttcg ggaatggctt tacccgccat gccagagtcc accgccgcta aaccttgcgc
    20581 acgagcgcgg aaagcagggt cggccagctg tttttgctgt tctgccagtt gcgattgtgc
    20641 agcagctacg ctatcgccat cgctaaagat cgagagatat tttttcagtg cctgaacgct
    20701 ggcatcgctg acaggcatgt ctttaatctg cccgtaccag attttagagg ccccttcgcg
    20761 cccggcgttc gatttcgcca tctgttccag gacggcaaaa ccttcatcac ggcgatcgct
    20821 actaaacagt aatagcgcca gattgttttg caggcccgtg ttgcccggcg tatccgcatt
    20881 aatgcgtttt agctgattaa tcgcttcgcc acgtcgggcc ggaattttcg ccaccgtact
    20941 ccagtactcg acagcaatgt caccttccgg cggcgcaccg ttgaacagtt tgttgtaact
    21001 cgccacagct tcttccgcat gaccagtcgt cgcctgcaat cttgcctgtt gcagtgcctg
    21061 acgaccatcc gacgtggaaa gcagcatcgt agtccgtgac gatttatacg catttgaact
    21121 cggcgctaac tgcgacagcc gatcgagctg tttttgtgcg ccatcaatat cgccctgacg
    21181 taacagagaa cgaaaacggg cagcaacgac gtccgggtta ttcggatcaa taagttccag
    21241 acgatacaac gactgttgca ccaggtcttc acgatgggtc gcttcgccta accgaacttg
    21301 ctccagcaac tgttgctgag cggttggtgc tgcctcgacc atcggcatga cggccagacc
    21361 gagggaaagc gtgaatatgt ttagtgtgaa tttgcgcatt cctggcccca gtcaggtaat
    21421 aactcacctt ttgtcgagaa gcggaaacgg tgttgatccc agccttgtcc aaacagggtc
    21481 agcacatagt tgtaataggc atcgctgcca ggaaagttat cggccacgcg ctggcgctga
    21541 acggcctgcg catcgcggtt ttgtaaaaag ggcagcatgg cggcagaaaa accgaccggt
    21601 cctttaccct gcgctttccc cgtagccaca tccacttttt ccggcggata accgtttttc
    21661 tcagtgaatg tcgccatcgg tttaaaccgg ttgagcatcc gcgctttttg cggatcgctg
    21721 tcaggcatca tgcctaccca catgtaaacg cggatagcgt cgtagctgct gatcaatgtt
    21781 ttttcggctt ttagctgcca gcctttgtct ttctcatagc gcacccagtc tggcgaaaag
    21841 cctttcgggg cggtttccag caataaacgt tgattggttt cgcgcagcgt ggtccacggc
    21901 gcgccaaagc gggtgaaata ctgcgccagc gtcggcggca ggtagctggg gttaaaacgc
    21961 cagctgttat cctctgcaaa tcccactttg cccggtaaca acatggagcc cagcccaggc
    22021 accgtcacca cttcctcccg cgcgatacgt tttagcaatg cgctgccgat gtcggtataa
    22081 cgctgctctt tccacaaacg ccccgcctcc agcaacgccc aggccatcca gacatcacca
    22141 tcggaggccg aattgctgtc cagcacttcc cacttactgt tctctttctt gccccacagc
    22201 caggcgggca aatgttcttt taaagaaccc tgtgcgagat tgttctgcgt ccagtcgaga
    22261 agattatcga aagctgcacg gtcgttagcc gccagggcaa agaacatgcc gtaactttgc
    22321 ccttcggagg tggtgatttt gcgcgcgtcg ctggggtcga tgacgcgccc ttcctgactg
    22381 atgtaatcct ttttaaactg ctcccaggca ggccaggtac aggctgcctg aacactaaag
    22441 gcagccagca gcagcatcgt cacgagtcca ctacgcaaca cattcatctt caattactcg
    22501 ttatccgggt taagacgacg acggctgata atacgcagca gacgccacag tacccatgcc
    22561 agcagcacga cgctgattgc cgccagcacc gccagcagaa tcggatggtt tgccagcgca
    22621 taccataagc gttcgaacca cggcagatgg ccaacgtaat agacgtcgcc aacacgcagg
    22681 ctattgatac cggactcgcg gatcaccgcg accgaaccga acatggtggc gcgtttgccg
    22741 ctatcgttca ccgcatcgtt aagcatttca taaccgcgtg ggctatctgc cagcagcgca
    22801 atcacgctac gctggtcgtt atacggcgac tggaagccaa tcaccgctgc catcgcacct
    22861 gaagaggtca gcgttgactg agtttctgcc gcgcgatcgc tctcgtccgg tacaatgccg
    22921 gggaacgggg tctggcgcat cggcgttttc acccagcttt cggtcgcctg caccaacagg
    22981 tcgatctgct tgtcgtcttt cagtttgtcc gggataccac cgacgatcat gatgtcggca
    23041 tctttcccct gaatagtgct gccatcatcg gtcaccgtca ggttaatcgc cgggaagccc
    23101 gtctgcgcac cgataaagcc aacggtattc agcaaggttt ccatctgtgc ttcgttaggc
    23161 gctttcggca tcacggtgat ggtttgtgac agatccgcca tccggctgaa cgggaagccc
    23221 gcgttagcaa aggcgcgtag atccggcatc gggatgaagt ggtaatactt cgagaagtcg
    23281 atggtggaat catcaccaat caccacatga ttctgcaccg gctggaaggt aatgcagtta
    23341 tccaccgaac cgcccggcat cgggttcata tactcaaagt caaagcgcag ctggttggtc
    23401 gcgcccagtt tcagcgccgg aatagagaca tccgttttgc catccagcaa accttgtaat
    23461 accggaatac gcagcagcag gcggttcgcc tcctgtttac tgctcaggtt gaaggattgc
    23521 aggaactggt tattcaggct gatatccatc cgtgagctgt ctttcactgg cggcatggtg
    23581 tagcgataat taatatccat atcaatgccg gtactgcgca tcaggtagag atccggtggc
    23641 aggtttagcg aaacgttaat cgctgctggc tcaagaccgc tggattgtaa ctgttcttca
    23701 taggttttca gttcaccaaa ggtaaccgga cgatcggtac gcacccagtt cggcgcatcg
    23761 tacggcttac gcggtagcag cggtttcact tcattcacta ccacgctttc accacggaac
    23821 agaatgttac cctgagcgat acctttcgct gcctgcaaca ggtctttgtc gtcacgacca
    23881 aacaccacca gcagtttgac ataaggattc tgcggatggt tgatcatttc aatcaccggg
    23941 gcttttaccg ccggatgatc gcgcaggaag tccgggcgtt tgtcattggt ggcaaaaacg
    24001 atggcattgc gatccggcaa ttggttatag agcaccggga agttctgccc acgccagcca
    24061 gaacgcgaac caaaccacga ggcgacaatg gcagatgctt gttgcagccc gacatccggc
    24121 gcacccgcaa agaccatcgg caaggtgtta gtacggttat cacgcgggtc aaagaatggc
    24181 accgggaagt gtgacaggtc attcttcaca ttcagggtct gataggtcag atccagtcca
    24241 ctgctccgcc caacatccag ccaaagcgtg gtgctggccg ggttttcgca cacgtcctga
    24301 taatgaccga caaactccag ccgcacacgg ttgaagtcgg taataaacag tgggttaatt
    24361 ggcatttgtg ccagcgtttt tttacccaac tgttctttgg tcactggcag cacgcccatc
    24421 agttcatcat tgagataaac ctttaactgc gactggacag gcagtaatga tggcgatggc
    24481 gtgtattcga ggttgagcat cgccttcgtc accacttcat cgctgcgcat accaaactca
    24541 atgctgccgt tcgggttaat gccacgtagc accatgctgc ccggcggcgg cgcaatttgc
    24601 gcaaaggtca gcttcacatc acgggaagga ccgttctgcg ccacgactgg cgcatctgcg
    24661 ccctgcacgc ccggcatcac ttgccccacc tgcggatttt gctccgcctg ggcggctaca
    24721 gctggctcag cattgatcag tggttgcgtt gctggcgtcg cctgcgtcat gaaagagggg
    24781 aacgcactca tccccagagc cactgcacaa atccagaata tttttctttt catcgcgtta
    24841 tcatcattgt tgagccaaag cctgatccga tggttgtgcc gtttcgctcc gctccgggcg
    24901 gcgcggaata aacgatacaa cccaggaaac cagagaagtc agcacacgga atatgccctt
    24961 caccgaagaa ggcgcaaatt ccgccagatg gcggtagcca cggaagccga gcttcagaat
    25021 atccagcaga ctttccagcg gcttatcttc cgggtagctg tcctgccaga gcgcccatgt
    25081 atccgcacgg gcaaacgtac actgcacaaa atcgatatgt tgctgggtag tgagcggcat
    25141 taatttcagc ccaacttcat tacccatcac acgcgccacc tgagccggga agacgtattc
    25201 ctgctgaccg cgtttaagca acagattcac tttctgccct tccagaatct gcgcctgacc
    25261 gttgatcttg atccccaaac caccgtcgga gaaatcctga acggtacacg agaagaggtg
    25321 accatcttcg cgggcaattg ctgcgggcat cgtcatctcc acgcggtgcg atcggcgtac
    25381 ctgtttgctt tctaccgata ctgcgactgc gccgccgaga ataatcaggt tgtagaatac
    25441 ccacaccata ctgacgacca cggtgagcat ctcggttggc ggaccataga agtagcgcca
    25501 gatcccaacc gctacgccca ccaggttgag cagaacaagg aagatgtagg gccgcgagat
    25561 cacccagtcg acgtactctt cttccaccag tccacctttg gcggtgacgt taaatttgcc
    25621 tttgtgcggg ttaatcagcg ccaccagcgt cggtggtgcg atataccacg ccagcactgt
    25681 ttcgtagatt tcactccaga aagagtggcg atatttgccc tggatcttcg agttagtcag
    25741 gctggcatgg atcatatgcg gtagcacgaa cagggcgatc atcaacgctg gcgcgtagat
    25801 gatgtaggca tgaaacagca ggaacgccag cggcgcagtc aggaagatca gccgtggaat
    25861 gcccgacaag aaatggaaca tggcgttgac atagcacagc cgctgagcaa acttcagccc
    25921 tttaccggtg agtgggttat cgagacggaa gatttgcacc atcccgcgcg cccagcgaat
    25981 acgctgaccg atatgcgccg agagactttc ggtcgccagc cccgctgcct gcggaatacg
    26041 catatacgct gaggtgtagc cacgacggtg cagacgcaga gaagtgtgcg catcttcggt
    26101 taccgtttcg acagcaatac cgccaatctc atccagcggc ttacggcgaa tcaccgcaca
    26161 tgaaccgcag aagaaagtgg catcccacat atcattgccg tcctgcacca gaccatagaa
    26221 cagcgtgcct tcgttcggcg ttttacgaaa acgccccagg ttgcgttcaa acggatccgg
    26281 cgagaagaag tggtgcggcg tctgcatcat cgccagctgt ttctctttca ggaaccagcc
    26341 catggtcatt tgcaggaacg atcgcgttgg tacgtggtcg cagtcgaata tcgacacgaa
    26401 ctcgcctttg gcatatttca gcgcattgtt gatgttgccc gctttcgcat gttcatgagt
    26461 ggtacgggcg atatatttca cccccacgtt ttgcgcaaac tggcgaaact cttccctgcc
    26521 gccgtcatcg aggatccaga tgttcagctt gtctttcggc cagtcgatac ccagcgaagc
    26581 gtaaatggta tttttcacca cgttgagatc ttcgttgtaa gtcgggacaa agatatccac
    26641 cgacggccac agcgacatat ctttcggcaa tggcaccggc tgacgattca gcggccatac
    26701 gacctggaag tagccgagca ccagcactat ccacgcgtac gtttcggcga acagcagaat
    26761 aagcccgcac accaggctga ccggatcgtc ccagtttaac gtcgaggtgt aacgccacca
    26821 gatataacgg caagaaacgg tcagcgacag cacaatcagc atcagcgccg agaagcgccc
    26881 cggcatccgc cgtacgatca gcgctacccc ccacagcagc atcaggaaga taaactgcgc
    26941 tagcgggtta aacggctgag taacgcagat taacgccaga atcagcgaga aggtgacgat
    27001 gataccaagg atcaaccgcc gcgccccggc actcaaatga ccgagctctt ttttctcatc
    27061 aagatgctgt gttttatggc taacgcgctc aggcagctcg ttcatccatt gatggtaacg
    27121 tccacgaata ttttgtagac ctgaaaatgc ccgcctgcgc ggtttcggcg tttctttgcg
    27181 cgatgcaccg atcagtaacc agcatgtttg aataagataa cggaccgggt ccagcggacg
    27241 cggacgcgag gcgttgatat gcggatacag gtttttatgt tctgcgcgaa tacgctgcca
    27301 gcgcgggtgc tccagcggaa taaaaatcca ggccaggatc atccagaaac agccgagcgt
    27361 cgcgctgaaa gccgacgcac cgtgacgacg ataatcgcga taacgcccaa taagccgtgc
    27421 gttgaccggc gggataagca accaccgggt caggatactc atgatgcact cccgactggc
    27481 gttttcagcc cggaataatt caacaggcac cagttcgcca gcgtcagtat ctcttcagcc
    27541 gccagcgcat cactgcgata ttcaccgact ggttgcttag ctgccagaca ttcagccatc
    27601 gcttcatcac gatgaatgag catcggcagc aatcggcgct ggctttgcaa ccaaagctgg
    27661 taaatatcgt cctgaacctg gctgccaata cggaagtcat taatcaaaat atgtgcacca
    27721 tccggcagcg cttgctgatg cagtcggata tggcagttgg catccacatt gacgattgcc
    27781 agcgagtgat cgcacaaact cagcagctgg tgggttatct gcgaggcatc acgcggtaag
    27841 tcaattaaaa tccactggta acgcccgctg gcttttagtt gctgtaagcc ggtgcaaata
    27901 tcgctcagcc gggtttgcca gtgctgtgga ttttcttgtt cttcaatcga taactgacca
    27961 aaaggcagca aatcgagctg cgaggtatag cgcaacccgg cgtcacgcca gtcctggcca
    28021 tccagcatag ctctggccca accctgacgg tgggtaaaat caacgttaaa tgacaggcgc
    28081 aacaagttgt ccgggcaggc atcgaccacc aggacatttt ctcccaacat ttgtaatgac
    28141 caggctaatg cggcggtgat ggtt
//
LOCUS       DAZKGF010000048        27925 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZKGF010000048 DAZKGF010000000
VERSION     DAZKGF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.048"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(84..1367)
                     /gene="yeaH"
                     /locus_tag="ACHOQV_001990"
     CDS             complement(84..1367)
                     /gene="yeaH"
                     /locus_tag="ACHOQV_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFP9630366.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1480..3414)
                     /gene="yeaG"
                     /locus_tag="ACHOQV_001991"
     CDS             complement(1480..3414)
                     /gene="yeaG"
                     /locus_tag="ACHOQV_001991"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFP9630367.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3850..4596
                     /gene="mipA"
                     /locus_tag="ACHOQV_001992"
     CDS             3850..4596
                     /gene="mipA"
                     /locus_tag="ACHOQV_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFP9630368.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYDGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4686..5540
                     /gene="yeaE"
                     /locus_tag="ACHOQV_001993"
     CDS             4686..5540
                     /gene="yeaE"
                     /locus_tag="ACHOQV_001993"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFP9630369.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYAEGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQIPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5591..6475)
                     /gene="yeaD"
                     /locus_tag="ACHOQV_001994"
     CDS             complement(5591..6475)
                     /gene="yeaD"
                     /locus_tag="ACHOQV_001994"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFP9630370.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKACEIDLEAHGEFETT
                     SALHTYFNVGDITKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6559..7554)
                     /gene="gapA"
                     /locus_tag="ACHOQV_001995"
     CDS             complement(6559..7554)
                     /gene="gapA"
                     /locus_tag="ACHOQV_001995"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9630371.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7896..8309
                     /gene="msrB"
                     /locus_tag="ACHOQV_001996"
     CDS             7896..8309
                     /gene="msrB"
                     /locus_tag="ACHOQV_001996"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP9630372.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8351..8623
                     /gene="yeaC"
                     /locus_tag="ACHOQV_001997"
     CDS             8351..8623
                     /gene="yeaC"
                     /locus_tag="ACHOQV_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFP9630373.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8784..9425)
                     /gene="pncA"
                     /locus_tag="ACHOQV_001998"
     CDS             complement(8784..9425)
                     /gene="pncA"
                     /locus_tag="ACHOQV_001998"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFP9630374.1"
                     /translation="MHPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIIMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(9436..10452)
                     /gene="ansA"
                     /locus_tag="ACHOQV_001999"
     CDS             complement(9436..10452)
                     /gene="ansA"
                     /locus_tag="ACHOQV_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP9630375.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(10617..12473)
                     /gene="sppA"
                     /locus_tag="ACHOQV_002000"
     CDS             complement(10617..12473)
                     /gene="sppA"
                     /locus_tag="ACHOQV_002000"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9630376.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            12634..13185
                     /gene="ydjA"
                     /locus_tag="ACHOQV_002001"
     CDS             12634..13185
                     /gene="ydjA"
                     /locus_tag="ACHOQV_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFP9630377.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAAMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            13302..14345
                     /gene="selD"
                     /locus_tag="ACHOQV_002002"
     CDS             13302..14345
                     /gene="selD"
                     /locus_tag="ACHOQV_002002"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFP9630378.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRHACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRELLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            14350..16305
                     /gene="topB"
                     /locus_tag="ACHOQV_002003"
     CDS             14350..16305
                     /gene="topB"
                     /locus_tag="ACHOQV_002003"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9630379.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVII"
     gene            complement(16382..17725)
                     /gene="gdhA"
                     /locus_tag="ACHOQV_002004"
     CDS             complement(16382..17725)
                     /gene="gdhA"
                     /locus_tag="ACHOQV_002004"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP9630380.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            17961..18233
                     /gene="ynjH"
                     /locus_tag="ACHOQV_002005"
     CDS             17961..18233
                     /gene="ynjH"
                     /locus_tag="ACHOQV_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1496 domain-containing protein"
                     /protein_id="HFP9630381.1"
                     /translation="MSRALFAVVLAFPIIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQQDDKNLSTNPLVWRRVKP"
     gene            complement(18199..18606)
                     /gene="nudG"
                     /locus_tag="ACHOQV_002006"
     CDS             complement(18199..18606)
                     /gene="nudG"
                     /locus_tag="ACHOQV_002006"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFP9630382.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            18693..19313
                     /gene="ynjF"
                     /locus_tag="ACHOQV_002007"
     CDS             18693..19313
                     /gene="ynjF"
                     /locus_tag="ACHOQV_002007"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9630383.1"
                     /translation="MLDRHLHPRIKPLLHQCVKVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALIVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(19322..20629)
                     /gene="ynjE"
                     /locus_tag="ACHOQV_002008"
     CDS             complement(19322..20629)
                     /gene="ynjE"
                     /locus_tag="ACHOQV_002008"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFP9630384.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSANWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKKRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDIEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(20696..21349)
                     /gene="ynjD"
                     /locus_tag="ACHOQV_002009"
     CDS             complement(20696..21349)
                     /gene="ynjD"
                     /locus_tag="ACHOQV_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9630385.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGGLAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRAQAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(21349..22884)
                     /gene="ynjC"
                     /locus_tag="ACHOQV_002010"
     CDS             complement(21349..22884)
                     /gene="ynjC"
                     /locus_tag="ACHOQV_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9630386.1"
                     /translation="MATPLRYALIFLLWAMMAVIYAPLIPAVLTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQAKGALASLLLMLLLAAYVLLGYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLTATFIALILIL
                     AWLEWGSSRRHFWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGYLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRSKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(22857..24023)
                     /gene="ynjB"
                     /locus_tag="ACHOQV_002011"
     CDS             complement(22857..24023)
                     /gene="ynjB"
                     /locus_tag="ACHOQV_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9630387.1"
                     /translation="MRHCVWLLGLLSLFSLATHASDWQEIKNDAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYDINLKIVHLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQFLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFSEGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(24033..24581)
                     /gene="ynjA"
                     /locus_tag="ACHOQV_002012"
     CDS             complement(24033..24581)
                     /gene="ynjA"
                     /locus_tag="ACHOQV_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9630388.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SIFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLVTHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(24581..25288)
                     /gene="ydjZ"
                     /locus_tag="ACHOQV_002013"
     CDS             complement(24581..25288)
                     /gene="ydjZ"
                     /locus_tag="ACHOQV_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP9630389.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLNSMDGFFTRYGKHTILVCRLLPFVPF
                     DPVSYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(25302..25979)
                     /gene="ydjY"
                     /locus_tag="ACHOQV_002014"
     CDS             complement(25302..25979)
                     /gene="ydjY"
                     /locus_tag="ACHOQV_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFP9630390.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(25984..26694)
                     /gene="ydjX"
                     /locus_tag="ACHOQV_002015"
     CDS             complement(25984..26694)
                     /gene="ydjX"
                     /locus_tag="ACHOQV_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP9630391.1"
                     /translation="MNAERKFLFACLIFALVIYAFHAFGLFELLTDLPHLLTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            complement(26861..27667)
                     /gene="xthA"
                     /locus_tag="ACHOQV_002016"
     CDS             complement(26861..27667)
                     /gene="xthA"
                     /locus_tag="ACHOQV_002016"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP9630392.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTANRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         6894 a         7099 c         7199 g         6733 t
ORIGIN      
        1 attgtaagct aatgtatgta ataaatgaga taatttacag tgaactaaaa atcagccagg
       61 cgataatgac ccggctgaca gttttagtct ttagctgttg cattttgttt atgaaacagt
      121 tcacggaaca ccggataaat atcatcctgg tcgcggatat gctgcatcgc aaagttgtcg
      181 aaagtagatt gcagatgctc atattctcgc cacagtgtct gatgcgcgcg acgtgtaatt
      241 tcgatatagc tgtaataacg aacaacgggt aatatttttt tcgccagaat ttcatggcaa
      301 agcggagagt catcggccca gttatcgccg tctgatgctt gggctgcgta aatattccac
      361 tgtgccgggt tataacgctc tttcactacc tcatccatca gtttcagggc gctggaaaca
      421 atggtgccgc ctgtttcctg cgagtagaaa aactcatgtt catcgacttc tttcgcctgg
      481 gtatgatggc ggatgtatac gacttccacg ttcttatatg ttctgctgag gaacagatac
      541 agcagaatat aaaaacgttt agccatatct ttagtggact gatccattga accggaaacg
      601 tccatcaggc aaaacatcac tgcctggctg gagggatcgg gccgtttttc gtagttcttg
      661 taacgtaaat cgaaggtgtc aatgaaaggg acgcgttcaa ttttggcacg taattctgca
      721 atttctttgc gcagacgttc ctcttccagc agttgcgcag gttcactgtt gctgatgatg
      781 gccaaattct cttccagcgc atgaagttcc cgccgcttgc ccgccgtcat ggctgtgcgc
      841 cgcgccagtg agttctgcaa tgaacgcaca acgctgatat tggccggaac gccgttagcg
      901 gtataacccg cccgatgcgt tttatattcg gtcagctggc gttgttggtt ttgtttcaga
      961 ttcggtaaag ccaaatcttc aaagagcaga tcaagatact catctttcga aatctgaaag
     1021 acgaattcat cctgaccttc accatcctgg ctggcctggc cctgaccgct gccggaacct
     1081 ccgccgccac cctggggacg ttcaattcgg tcgttctgga cgaaatggtc attgcccgga
     1141 tgcacgcggt ggcgcagacc gccacgcccc tgatgaaaca tcggttcgct aatatcttcc
     1201 gtgggaatgg atacggactc gccgctgtcg acgtcagtca ccgaacgctt attaatggcc
     1261 tcggagatcg actgtttaat ttgcgcttta taacggcgta aaaaacgctg gcgattcacc
     1321 atgcttttgt ttttgccgtt cagacgccgg tcaataaacc aggtcatatg ccccccgtac
     1381 tacatttgcc aactttatgc tacgcctgtg ttgccgcttc tctggcccac ggttcgctgt
     1441 cgttgtaggc ccggtagcat gtgtaccggg ccagggttgt taagacgatt tacgtacgcg
     1501 caaataccat tcgcacagta aacgcacctg tttacgggtg tagccttttt ccatcatacg
     1561 gtcgacaaag tcgtcatgtt tcttttgctc gtcggttgac gttttggcgt taaacgaaat
     1621 aaccggcaac agctcctcgg tattggagaa cattttcttc tcaataaccg tgcgcagttt
     1681 ttcatagctg gtccagttcg gattgcgtcc gctgttattc gctctggcgc gcagtacgaa
     1741 gttaacaatc tcgttgcgga aatcttttgg attactgatc cccgccggtt tctcgatttt
     1801 ctccagctcg gcgttaagag actcgcggtc aaacaactgc ccggtatcag gatcgcgata
     1861 ctcctgatcc tgaatccaga aatccgcgta ggtaacataa cggtcgaaaa tgttttgccc
     1921 atattcggaa taggattcaa ggtaggccgt ctggatctct ttgccgataa actcggcata
     1981 tttcgggatc agataacctt tcaggaactc cagatagcgt tctgcctgtt cttgcgggaa
     2041 ctgctcgcgc tcgatctgct gttccaggac gtagaacaga tgaaccgggt ttgccgccac
     2101 ttctacatga tcgaagttga acacgcggga gaggatctta aacgcaaaac gcgtcgacag
     2161 accgttcatc ccttcatcga caccggcgta gtcacgatat tcctgatacg acttggcttt
     2221 gggatctgtg tctttcagac tttcgccatc ataaacccgc atctttgaat aaatgctgga
     2281 gttttctggc tctttaaggc gcgaaagaat ggaaaaacgt gacagcgttt ccagcgtgcc
     2341 aggggcgcag ggggcgtgag tcaattcact gtgattaagc aatttctcgt agattttgat
     2401 ctcttcggaa atgcgcaagc aatacggcac cttcacgatg taaacacgat cgaggaaggc
     2461 ttcgttgttt ttgttattac ggaaagtgac ccattcggat tcgttcgagt gggcgagaat
     2521 aatcccgttg aacggcaggg cggagatacc ttccgtcccg ttgtagttac cttcctgagt
     2581 ggcggttagc aagggatgca gcactttaat cggtgcttta aacatctcaa cgaattccat
     2641 gatcccctgg ttggcgcggc acagtgcacc ggaatagccg taggcgtccg ggtcattctg
     2701 cgcgtagtgt tcgagtttac gaatatcgac tttcccaacc agcgcggaga tgtcctggtt
     2761 gttctcatca ccgggttccg ttttggcgat agcaatttgt tgcagaattg acggccagac
     2821 cttcactacc cggaacttag tgatatcgcc accaaattca tgcaggcgtt ttgccgccca
     2881 cggcgacatg atggtgccga gataacggcg aggaatgcca tactcttttt ccagaatctg
     2941 cgcatcttcc tgcggattga aaagacagaa cggatgatcg ttgaccgggc tacgctcacc
     3001 gttggcgctc aatacataaa ttggtacgag ctgcattaat gatttcagtc gctcagcaag
     3061 cgatgattta cccccaccca caggcccaag cagatacagg atttgtttct tctcttccag
     3121 cccctgagcc gcgtgtttca gataagagac aatctgttca atcgcgtctt ccatgccgta
     3181 aaactcttca aatgccggat aacgtgctat gacccggtta gaaaagagtc gagaaagtct
     3241 gggttcctgg gctgtatcga ccatgacagg ctcaccgata gccatcaata gccgctcagc
     3301 cgcgttggca taagcactgc gatcttgccg acaagtggta agaaactcct gcagtgtgaa
     3361 ctcttcgtcc ttggcagctt catatcgctg gcgatagtga tcgaatatat tcatggtatg
     3421 ccgtcctttc gttttttagc acaggttaag agccgttcat atgaatagta gaggctcccg
     3481 gaagaagtaa actgaacgac gtctcttgct aaaacacagc aactctcatg ccattttatc
     3541 gacgatgaga acaacgtgcc cggtgataca ccttcaaaat taagcgtaga tggcaaatgg
     3601 aaaacttgcc tgcacggctt tcttaatttc aatggcatat caacaactca tccataaatt
     3661 ttgcataatt aatgtaaata ccagggtcgc cggtaacgct aaattcattt ggctgtaaga
     3721 gtggtgtcat ctgcgtcagg aaaattaaac agttactata aaaaatgaaa acgtaaaaag
     3781 gttgggtttc gatgtattga cgggtaaact ttgtcgcccg ctaaacattt gtttaaggaa
     3841 tgattaattg tgaccaaact caaacttctg gcacttggag tgcttatcgc aacgtctgca
     3901 ggcgtagcgc acgctgaagg taaattttcc ctgggcgcag gcgtaggtgt cgttgaacac
     3961 ccatataaag attacgatac cgatgtttac ccagtaccgg taatcaacta tgacggcgat
     4021 aacttctggt tccgtggctt aggtggtggt tactacctgt ggaatgacgc aacggacaaa
     4081 ctttcaatta ccgcttactg gtcgccgctt tacttcaaag ctaaagacag tggcgatcac
     4141 caaatgcgtc acctggatga ccgtaagagc accatgatgg ctggtctgtc ttatgctcac
     4201 tttacccagt acggttacct gcgtaccacc ctggctggcg ataccctgga taacagcaac
     4261 ggcatcgtct gggatatggc ctggttgtat cgttacacca acggtggcct gaccgtgact
     4321 ccgggtattg gtgtgcagtg gaacagcgaa aaccagaacg aatactatta tggcgtatcg
     4381 cgcaaagagt ccgctcgcag cggtctgcgt ggctataacc cgaacgacag ctggagccct
     4441 tacctggagc tgagcgccag ctacaacttc ctcggcgact ggagtgttta cggtaccgcg
     4501 cgctacaccc gtctgtctga tgaagttact gacagcccaa tggtggataa atcctggact
     4561 ggcctgattt ctaccgggat cacctacaaa ttctgataat gcatctttcc agggcatttt
     4621 gtgcataaaa acagggcgtc atgccctgtt ttgcattacg gtggtgcaat acctgggagg
     4681 aaacaatgca acaaaaaatg attcaattta gtggcgatgt ctcactgcca gccatagggc
     4741 agggaacatg gtatatgggc gaagatgcca gtcagcgcaa aacagaagtt gctgcactac
     4801 gcgcgggcat tgaactcggt ttaaccctca ttgataccgc cgaaatgtat gccgaaggcg
     4861 gtgctgaaaa ggtggttggg gaagcattaa ccggtctgcg tgataacgtc tttctcgtct
     4921 ctaaagtcta tccgtggaat gctggcgggc aaaaagcgat aaatgcatgc gaagccagtt
     4981 tacgccgtct caatactgat tatctcgatc tctacttatt acactggtct ggcagtttcg
     5041 cttttgaaga gactgtcgca gcgatggaaa aattgatcgc ccagggaaaa atccgccgct
     5101 ggggcgtttc taaccttgat tatgccgaca tgcaggaact ctggcagctg ccggggggaa
     5161 atcagtgtgc cactaatcag gtgctttacc atctcggttc acgagggatt gagtacgatc
     5221 tactcccctg gtgccagcaa cagcagatac cggtgatggc ttacagtccg ttagctcagg
     5281 ccgggcggtt gcgcaatggg ctgctaaaaa acgcagtagt caacgaaatt gcacatgctc
     5341 acaatatcag cgcggcacaa gtattgttgg cgtgggtgat cagtcatcag ggtgtgatgg
     5401 cgattccaaa agcggccacg attgcccatg tccaacaaaa tgcggctgtg cttgaggttg
     5461 aactttcgtc agcggaatta gctatgctgg ataaggcata tccggcacca aaaggaaaaa
     5521 ctgcgctgga tatggtgtga tgttgatacg tgccggatgc aacgcacccg gcacacaaca
     5581 ttaacgcaaa ttaacgtttc gcaacgcgaa tggattgcgc cagatgtgca ggtttttctt
     5641 tggtcacttt ttgcgtttct gaagcgtaag ccgtttctac acaaacaaag gttttgtagc
     5701 catcgtccgg catatcgccc atgctaactg aaagcgccgg tcctgggttc cagccaacga
     5761 cgttcagatg atgctggtgg cctacggcga taatacgatt cagcgcttcg tcattaatca
     5821 cgctgcaatc ttgcggattc agatacacgc gatcggtacg gtcagggaag gtctgaatac
     5881 cgtcggtcag tacatcttct ttcgcgtcat tcactttatc aatgaagcga tcgcccagtc
     5941 cactgacgct taccttagtg atatcaccca cgttaaagta ggtatgcagg gcagaggtgg
     6001 tttcaaattc gccgtgtgct tcaagatcga tttcacaggc tttacccacg cggaaatgcg
     6061 ctaatagcgt aaagtcgtgc ggccagaatt ttttcgtctc ttcgctttgt gtcaattcaa
     6121 aagtcagcgc tacgccatca gcatcttcac gatgtgattt cagcgtccac ggcaggttgc
     6181 gggcaaaacc gtgcgcaggc agaccttgtt gtgcagccgg accaaaccac ggccagcaaa
     6241 ccggtacgcc accgcgaata gcgacgccat ttttgaacgg tgtgttattg ctcaaccaca
     6301 gaacttcttc ttcacccgca ggtttccatg agagaaggtg tgcgccctgt aatgcaaaag
     6361 aggcttttac ctggggatga tcgaccacaa tgaggtccag ttcatccagt ttacgacggg
     6421 agaggacagg ggagatttgt tcgatgaccg gaagggcaaa aattttctta atcatgacgc
     6481 agtcctttaa cttcatttta tcaggtaaaa aaaagagcga ccgaagtcgc tctttttaga
     6541 tcacagtgtc atctcaactt atttggagat gtgagcgatc aggtccagaa ctttgttgga
     6601 gtaaccggtt tcgttgtcgt accaggatac cagtttcacg aagttgtcgt tcagagcgat
     6661 accagcttta gcatcgaaca cggaagtgca aacttcgccg ttgaaatcgg tagatactac
     6721 gtcatcttcg gtgtagccca gaacgccttt catttcgcct tcagcagcag ctttaacggc
     6781 agctttgatc tgctcgtaag ttgcagcttt ttccagacga acggtcaggt caactacgga
     6841 tacgttcggg gtcggaacgc ggaacgccat accagtcagt ttgccattca gttctggcag
     6901 tactttacct acagctttag cagcaccggt agaggacggg atgatgttct gggaagcacc
     6961 gcggccgccg cgccagtctt tgtgagacgg gccatcaacg gttttctgag tagcggtagt
     7021 agcgtgaacg gtggtcatca gaccttcgat gatgccgaag ttatcgttga taactttagc
     7081 cagcggagcc aggcagttgg tggtgcagga agcgttggaa acgatgtcct ggccagcata
     7141 tttgtcgaag ttagcgcctt taacgaacat cggagtgttg tctttagacg gaccagtcat
     7201 aaccactttc ttcgcgccag cggtgatgtg tttacgagca gtttcgtcag tcaggaacag
     7261 accagttgct tcagcgacaa cgtcaacacc aacttcgtcc catttcaggt tagccggatc
     7321 acgttcagcg gtaacacgga tttttttacc gttaacgatc agatgaccgt ctttcacttc
     7381 aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc agcatgtatg ccatgtaatc
     7441 agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca gaacgtttct gagcagcacg
     7501 gaaaacaatg cgaccgatac ggccaaaacc gttgatacct actttgatag tcatatattc
     7561 caccagctat ttgttagtga ataaaaggtt gcctgtaaaa ttacaaaaac cttacgcagc
     7621 gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc tgtgcctaag cattacgcga
     7681 ctgactcgcc tcactcttcc tttgggcttg agaccacatg gggtcggcgc cccgaatttt
     7741 aaagggcaat tacgataaaa atgtgatttt catcacgatt taggtgcagc catttcgtcg
     7801 agatcaagtt taggacaaaa ttttgccagt tggtgttaat gttttgttag aatcagtcac
     7861 gtaatgtgag cacgattaaa gtgagatgtg agcaaatggc taataaacct tcggcagaag
     7921 aactgaaaaa aaatttgtcc gagatgcagt tctacgtgac gcagaatcat gggacagaac
     7981 cgccatttac gggtcgttta ctgcataaca agcgtgacgg cgtatatcac tgtttgatct
     8041 gcgatgcccc gctgtttcat tcccaaacca agtatgattc cggctgtggc tggcccagtt
     8101 tctacgaacc ggtgagtgaa gaatctattc gttatatcaa agacttgtca catggaatgc
     8161 agcgcataga aattcgttgc ggtaactgcg atgcccatct gggccatgtc ttccctgacg
     8221 ggccgcagcc aacgggcgaa cgttattgtg ttaactctgc ttctttacgc ttcactgatg
     8281 gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc agcaacttat tccacaggag
     8341 cggaaaaatt atgaatcttg atgacattat caacagcatg acgcctgagg tataccagcg
     8401 tttgtcgacc gccgttgaac tggggaaatg gcctgatggc gttacgttga ccgaggaaca
     8461 aaaagagaac tgcctgcaac tggtgatgct gtggcaagcc cgcaataata ccgaagcaca
     8521 gcatatgaca attgacacaa atggtcagat ggtcatgaag agcaaacaac agttaaaaga
     8581 agattttggt atctcagcaa agcctattgc catgtttaag tgaatgagtt gtctagtatt
     8641 taagcctgtt attaagggag ggcaaataga cttgttcgga tgtcgtgttt tagctgcgag
     8701 taggttaaat gcaacactaa caagtattac ctggcctaca atgtcattga ggaaatgtag
     8761 gccagagatg aatccaacta atattactcc tgcgtctctt cccaatctgc cagcgtatat
     8821 agcgttgccc cagctgctga catctccata aacgcgtgcg cactgtcctg tggctggata
     8881 ttcacgccac gacaaccatc ggtaatcaca ttaaccttat aacctaactg taacgcgtcc
     8941 agcacggtaa acttcacgca atagtcagta gccagaccca taatgatcaa ttcatcgatt
     9001 tcatgatcgc gtaaccagtc atcgagcgtg gttttctgcc gacggccgtt atcaaaaaag
     9061 gcactgtaac tgtcaactaa aggattttcg cctttatgga acaccgctgc gatcgctttt
     9121 tggttcagta acggatgtaa ttgtgcgcct tcactgttct gcacacagtg atctggccag
     9181 aaggtttgtg gcaaaccgtc gagttggcct ggcgtataag gctctacacc gtgctgactg
     9241 gcaaaactgc cgtgattcgc cgggtgccag tcctgactgg cgataaccgc ttcaccgcgc
     9301 gactggcacc agtcaatcag gcggttagcg acatccaccg tactgtcacc ttccggcacg
     9361 gcgagcgcgc caccagcaca gaaatcattt tgtaaatcga ccagtaacag ggcgcgaggg
     9421 tgcattgcag tctccttaat catccggcgt cagttcgcca cgcaggtttt ggctcatggc
     9481 cttgcgaatg gtttcagtat caagttcctg gctaagcagg taatgcagtt tggttagcgt
     9541 ggcttctaca gtcatatctg caccgccaat aacgccggca tgggcgaggg cgttaccggt
     9601 ggcataacca cccatgttca ctttaccgga catacattgt gtcaggttga ccaccacaat
     9661 accgcgatcg ctggcttctt gtaattcctg caggaaggct ttgttttgtg gcgcattacc
     9721 cacgccatat gagcgcagaa tcaatgcttt cacaggttgg cgcagaaaat tgcgcacgac
     9781 gtcagcagaa atccccggat aaatcgtcac tacgccaatt ggttgcggcg tgattggatg
     9841 aacgatcaat tccccttcac cgtgcggggc gggtggcgta ttcaaacggc gaatatggat
     9901 acctgcttcc agtaacggag gaaggtttgg agaggcaaac gcatcaaaac catcggcatg
     9961 ggctttggtg gttctgttgc cacgatacag tcgattatta aaaaagagag ttacttcgtt
    10021 gataggataa ttcgccgcaa cgtacaacgc attcagcaga ttaatttgtc cgtcagagcg
    10081 taactcagcc agcgggattt gtgaccctgt cacaatgacc ggtttaccga gattctcgag
    10141 catgaacgac agtgcagagg cggtatacgc catcgtgtct gtgccgtgca gaatgacaaa
    10201 accatcatag tcgtcatagt gcgctttaat atcttcagca atatgctgcc agtcttctgg
    10261 cgtcatatca gaagaatcca tcaatggcgt atattcatga atagtgaaat ctggcatctc
    10321 cgggcgatgg aattccggca tcagcgccag ttggcgctgt agatgacctg acaccggaat
    10381 ataaccctgc tcggaacgct gcatcccgat ggtcccgccc gtgtaggcga cgtaaattga
    10441 tttcttttgc atgatattga tatgaatgat atcgaaagag caaaagtata tacgtgagga
    10501 agggggagcg cacctgatac tatgcggata aggaaatatg gggcgagaca gcgggaagag
    10561 gattcgtcga ggtaaaaaaa tgtggccgac gatctgtcgg ccactcagta caagacttaa
    10621 cgcacgttgg cgcaggtcag gcaaaacgca taacggtttt gtgggtcgtt aaacgctgcc
    10681 agcttgtcgc tttcactttt aacggtagag gctaccgagg ccagcggtgc aggtaacatg
    10741 gcctggaacg catctggcaa cattgcccgg acagaaccag acatgttgtc catcactttg
    10801 tcgaagaagg tcggttcatc aacgtagtat tccagatgcc actgtttcac ttttgccagc
    10861 tctgctgctt tggcgaccgc atcatcgaaa tccccgagac tatcgaccag cccgttagct
    10921 tttgcatcct gaccggtcca gacatggccc tgggcgattt tatcaatctg ctctggcgtt
    10981 gaatgacgcg catcagcaac cagcgtgata aagcgtttat agccattctc aatgcttaac
    11041 tgcatcatct gctgcgcttc cggcggcagt gccctggtga tagaaacatc cgccagcggt
    11101 gaagttgaga caccatcagt atgaacacca atcgaatcca gactattttc tacggtggtg
    11161 atcacgccga agataccgat agaaccggtc agggtgctgg ggttagccac aatgtaatta
    11221 gctggtgtgg aaatccagta accaccagat gccgccatgc cgcccatcga tacaaccaca
    11281 ggcttacccg ctgcccgggc tgctgccagt tcagcgcgaa tcacttcaga cgcggtaacg
    11341 ctgccgcctg ggctattaac acgcaggaca atcgctttca ctttcgggtc aaggcgagcg
    11401 tcgcggattt gtgccgcagt ggtatcaccg ccaacattcc cctgagtttc ctcgccatcc
    11461 ataattgcgc cattagcgaa gacgactccg atgctgtcac cggtatctgc cggcgttttc
    11521 aatgcgtaat cgtaataact gatggcgcga taatttttat cagtcttgct ccagccgaac
    11581 tctttggtca gagttttttc gatttcggca ctcgatgcca gtgcatcgac cagcttgttt
    11641 tccagcgcat atttcgcggt atcgccaccg gttttggtta aaccctcaag caacccttgc
    11701 gcgccaggga atacctgctg agcagggatc tgccggttag cggcaacagt attcagatag
    11761 ttttgccaca gctcaccaat ccagcggctg tcagcttcgc gggctgccgg cgacatatca
    11821 tcacgaataa acggttcaac ggcagattta tacgtaccca cgcggaacac atgggtggaa
    11881 actttcagct tatccagtaa tgatttgtag tacagaccgt tggtggcaaa gccgtgcaga
    11941 tcaaccacgc cttgcggaga cagccaaatt ttattggcga aactggcgag ataatattgc
    12001 ccctggctgt agttctcgcc aacggcataa accggtttcc cgctatcacg aaactctttc
    12061 agagctttgc cgatgtactg catagacggc tggtcgccgc ctgcgaagtt tttcagatcc
    12121 atcacaatac cggtgatatt gcggtcgtcc ttcgcctggc gaatggtgtt gacgatatca
    12181 aacagtgagt tttcctgcag acgatcggaa ctggcaccaa gcagctggcg gcttaattta
    12241 ctaaaccgct gagaactgtc gggtttatcg acgatcacac cagaaatgtc cagcagcagt
    12301 gcgccacgac tggccgtttc tttcgaatca ccaccactga cctgcatcca gatccccaca
    12361 ccaaccagca cgagaaaaat aaagaacagg ttaagtacca tttcacggac gaaattcagc
    12421 agacgccacg tccatttaaa aaatccggca ataaatcgcc aaagggttcg catgtattct
    12481 cccaacttaa ggtcacacct gtttcaggcg caatagatgt ggttatcgta atgacccaac
    12541 cggcacttgt cagcaggaat tgcggactgg gctgtaacaa aatccactct cgtgttaatt
    12601 ttgtgaataa atatcatgac aggagttaat caaatggatg cactcgaact attgatcaat
    12661 cgccgtagcg cctcccgcct ggctgaacct gcgccaacgg gtgaacaact gcaaaacatc
    12721 ctgcgtgcgg ctatgcgtgc gccggaccat aagtccatgc aaccgtggca tttctttgtg
    12781 attgaagggg aagggcgcga gcgtttcagc gccgtactgg agcagggggc gattgctgcc
    12841 ggtagtgata acaaagccat cgacaaagcg cgtaatgcgc cgttccgcgc accgctcatc
    12901 ataacggtgg tggcgaaatg cgaagaaaat cataaagtcc cgcgctggga acaggaaatg
    12961 tctgcgggat gcgcggtcat ggcgatgcaa atggcagcag ttgcccaggg gtttggcggc
    13021 atctggcgca gtggtgcatt aactgaaagt ccggtagtgc gtgaagcatt cggttgccgt
    13081 gagcaggata aaattgtcgg ttttctctac ctcggtacgc cgcagttgaa agcatctacg
    13141 tcgataaacg tcccggaccc gacgccgttt gtgacttatt tctgataatt gtcgcgaaac
    13201 tgtctggatt gtgagcaaag gcgcaggaat tcagacactc tcacttatca cttcatggaa
    13261 tgagggttac catacccgga ttgcattgac aggagatgtc catgagcgag aactcgattc
    13321 gtttgaccca atacagccac ggcgctggtt gcggctgtaa aatttcccca aaagtgttgg
    13381 aaaccatcct gcacagtgag caggcgaagt ttgttgatcc gaatttgctt gtgggtaatg
    13441 aaacccgcga cgatgcggcg gtatacgatc tgggcaatgg caccagcgtt atcagtacca
    13501 ccgacttctt tatgccgatc gttgataatc ctttcgattt tggccgcatt gcggcgacta
    13561 acgccatcag cgatatcttc gcaatgggcg gcaaaccgat tatggcgatt gcgatcctcg
    13621 gctggccgat taacaaactt tccccggaaa ttgcccgcga agtgaccgaa ggtggacgcc
    13681 atgcatgccg tcaggcgggg attgcgctgg ctggcggtca ctccatcgat gcgccagagc
    13741 cgatttttgg tctggcggta acggggatcg tacctaccga gcgggtgaag aaaaacagta
    13801 ccgcacaagc cggatgcaaa ctgttcctga cgaaacctct ggggattggc gttcttacca
    13861 cggctgagaa aaaatcactg ctgaaaccag aacatcaggg actggcgacg gaagtgatgt
    13921 gccggatgaa catcgcaggc gcgtcctttg ccaacatcga aggcgtaaaa gcgatgaccg
    13981 acgttacggg ttttggttta ctggggcatt taagcgaaat gtgtcagggc gcaggtgtgc
    14041 aggcgcgcgt cgactatgac gcgatcccga aattgccggg ggttgaagag tacattaagt
    14101 tgggcgcagt gcctggcggc accgaacgta actttgccag ctacggtcat ctgatgggtg
    14161 aaatgccgcg tgaagtgcgc gaactgctgt gcgatccgca aacttctggc ggtttgctgc
    14221 tggcggtcat gccggaagca gaaaatgagg tcaaagctac agccgccgag tttggcattg
    14281 aactgacggc gattggcgaa ctggtgccag cgcgcggcgg tcgtgccatg gttgagatcc
    14341 gttaattcaa tgcggttgtt tattgccgaa aaaccgagtc tggcgcgcgc cattgctgat
    14401 gtcctgccta aaccgcaccg gaaaggcgat ggctttatcg agtgcggtaa tggtcaggtg
    14461 gtgacctggt gtatcggtca cctgcttgag caggcgcagc cagacgccta cgacagccgc
    14521 tatgcgcgct ggaatcttgc ggatttgccg attgtcccgg aaaagtggca attacagccc
    14581 cgaccctccg tgactaaaca acttaatgtc atcaaacgct tcctgcatga agccagcgaa
    14641 atcgttcacg ccggagaccc ggatcgcgaa gggcaactgc tggtggatga agtgctggac
    14701 tacctgcaac tggcaccgga aaagcgccag caggtacagc gttgtttgat aaacgacctg
    14761 aacccgcagg cggttgagcg ggcgatcgat cgtcttcgct ccaatagcga gtttgtaccg
    14821 ctgtgcgttt ctgcactggc gcgtgcgcga gctgactggc tgtacggcat taatatgacc
    14881 cgtgcgtata ccattctcgg tcgcaatgcc ggttatcagg gcgtgctttc cgtgggacgc
    14941 gtgcagacgc ccgtactcgg actggtggtg cgccgcgacg aagagattga aaacttcgtg
    15001 gcgaaagact tctttgaagt caaagcacat atcgtgacac ctgccgatga gcggtttacc
    15061 gctatctggc aaccgagcga agcgtgtgaa ccgtaccagg atgaagaagg gcgcttatta
    15121 catcgtccac tggcggagca tgtggttaac cgcattagtg gtcaaccagc tattgtcacc
    15181 agctataacg ataaacggga atcagaatcc gcgccgctgc ctttttcgct ttcggcgttg
    15241 cagattgaag cggctaaacg ttttggtctg agcgcgcaga acgtgcttga tatctgccag
    15301 aagctgtacg aaacgcacaa gctaatcact tatccgcgtt ccgattgccg ctatttgcca
    15361 gaagaacatt ttgccggacg ccacgcggtg atgaatgcta tcagcgttca tgcaccagat
    15421 ctgttgccgc agccagtggt cgatccagat atacgcaacc gctgttggga tgacaaaaaa
    15481 gtcgatgcgc accacgccat catcccgacc gcacggagtt ctgcgatcaa cctgacggag
    15541 aacgaagcga aggtctataa cctgattgcc cgtcagtatt tgatgcagtt ctgcccggat
    15601 gcggtgttcc gcaagtgtgt tatcgaactg gacattgcca aaggcaaatt tgtcgctaaa
    15661 gcccgttttc ttgctgaagc aggctggcgc acactgttag gcagcaaaga gcgcgatgaa
    15721 gaaaacgacg gcacgccgct gcctgtggtg gcgaaaggcg atgagttgct gtgtgaaaaa
    15781 ggtgaagtgg tagagcggca aacccagccg ccgcgccatt ttaccgatgc aacactgctt
    15841 tcagcgatga ccgggatagc acgttttgtg caggacaaag atctgaaaaa gatccttcgt
    15901 gcgaccgatg gtctggggac agaagcgact cgcgccggga ttattgaact gctgttcaag
    15961 cgaggcttcc tgaccaaaaa agggcgctat atccactcca ccgacgccgg aaaagcgcta
    16021 ttccattcgc tgccagaaat ggcgacgcga ccggacatga ccgcgcactg ggaatcggtg
    16081 ctgacgcaaa tcagcgaaaa gcagtgtcgc tatcaggact ttatgcagcc gctggtgggg
    16141 acattgtacc agttgattga tcaggcaaag cgcacgtcgg taaggcagtt tcggggaata
    16201 atggctcccg gcggtggaga agggaagaaa aaggattcgc cacgcaagag agcgccgaaa
    16261 aaaagcccgc catcagaaga ggcgggcaat ggagtgataa tataaaggtt ctttcaacca
    16321 tctgatactg aagattttta tggccggata aggcgtttgc gctgtatccg gcacaattgt
    16381 cttaaatcac gccctggctt aacatcgcat ccgcaacctt cacaaaaccg gcaatgttcg
    16441 ccccctgcac gtagttggtt tgctcacctt caccaccatg ctcaacacag gcatggtgga
    16501 tatccagcat gatgtgatgc aaacgtgcgt caactttctc ggctttccag cccaggcgcg
    16561 cagcgttttg cgccatttcc aggcccgatg ttgctacacc gccagcatta gccgctttac
    16621 ccggtgcaaa cagtacgcct gcctgctgga acagttcagt cgcttcgatg gtggtcggca
    16681 tatttgcccc ttcggcgacg gctttaacgc cattggcgat aagctgatgc gcggcgtcaa
    16741 catccagttc gttctgggtg gcgcaaggca gggcgatatc aaccggtaca gaccacggtt
    16801 gttggccttc gagatagacc agaccaaatt ctttggcgta atcagccact cgaccatcgc
    16861 ggctggcttt gatttcgata agacgtgcca gtttctcttt cgtgaatccg ctttcatcaa
    16921 ctacagtgcc gctggagtct gacgcagtga tcacacgagc accaaattcc atcgcttttt
    16981 cgatagcgta ctgggcgacg ttgccggagc cagaaacgga aactcgcatc ccttcaaaac
    17041 ccataccgtg gcgttttagc attgcttcgg tgaaataaac cagaccgtag ccggtagctt
    17101 ccgggcgaat aagactgccg ccaaatgaaa ggcccttacc ggtgaagacg caggcggtat
    17161 tgttggagag ctttttcatc atccccgcca taaagccgac ttcacgacca ccaacgccga
    17221 tatcacctgc cggaacgtca gtatccgcgc ccaggtggcg atacagttca gtcatcagcg
    17281 cctggcaaaa acgcataact tcgccttcgc ttttcccttt cgggtcgaaa tcgctgccgc
    17341 ctttaccacc gcccatcggt agagtagtca gggcattttt gaaggtttgt tcgaagccga
    17401 ggaatttgag aatggagagg ttaactgacg gatggaagcg cataccgcct ttgtacgggc
    17461 cgatggcaga gctgaactgc acacgccatg cacggttgac ctgcacctgg ttgcgatcat
    17521 caacccatac tacgcgaaat tggatcacgc gctccggttc aaccagacgc tccagtaatg
    17581 acatctgacg atatttggga ttttgttcaa gaaaaggcca gagtgtggtc attacttcac
    17641 gaacggcttg cgcgaactcg gtttgattcg ggtcgcgctt ttggacatgg ttgaggaatg
    17701 actccagaga atatgtctga tccatagata taaaaccctt atatattaat acgattatgc
    17761 ttatgttgtt tatgtcatgt gcttttgcag ttttcgacta taccatcaag aatgtgacgg
    17821 taagcaagaa aaaaatgacc caggaaagca aattaataac gagagtaatc tcataaccaa
    17881 atgcgataac tcagacgcta aagttttgcc acccggctac cgttatcagt agcagagaca
    17941 ataacgttaa ggagttccct gtgagtcgag cattgttcgc cgtagtatta gcatttccaa
    18001 taattgcact ggctaacccg cattatcgtc cggacgtgga ggtaaacgta ccgccagaag
    18061 tgttcagttc aggcgggcag tcagcgcagc cctgtactca gtgctgcgtt tatcaggatc
    18121 aaaattattc tgaaggggcg gtaattaaag ccgaaggaat actgctgcaa tgccaacagg
    18181 atgacaaaaa ccttagcact aatccgctgg tctggcggcg cgtaaagcca taaacgcctc
    18241 taataatgga atgtcagcag gggccagcgg atattgcagc gcctcttcag gtgagcacca
    18301 gaccagcgcc tgatgttcat gtgcctgtaa cgtcccgtgg aagtcgggta cgtgccaggc
    18361 atgaagatgg ataatccgcc ccgaaacttc tcgctgatgg ctggcaacat agtcaccaac
    18421 agttgcttcg atgcccagtt cttcgtttaa ctcacgcacc agcgcctgcc gctggctttc
    18481 atccggctcg actttaccac cggcaaactc ccataatccc gcctgatcgc tctgggcggg
    18541 gcgttgcgcg agtaaaattt tgccatcacg ttcaatgatg gcggcaacaa cttcaatcat
    18601 tttcataaat tacacacggg cagataagcg aaaaatgcca taatatccgc actgttgctc
    18661 aatcggaaag gctttatatt taaggagtgc ctgtgctaga ccgccatctt catccccgaa
    18721 ttaaaccttt gttgcatcag tgtgtgaagg tgctggataa acccggcatc acgccagatg
    18781 gtttaacatt agtgggattt gccatcggcg tgctggcact gccgtttctg gcgctgggct
    18841 ggtatctggc ggcgctaatc gtcattttgt tgaacaggct gcttgatggt ctggacggtg
    18901 cgctggcgcg gcgcagagga cttaccgacg cgggcggctt tctcgatatt tctctcgatt
    18961 ttctctttta cgcgctggtg ccgtttggtt ttattctcgc cgcaccagaa caaaacgcgc
    19021 tggccggagg ctggctgttg tttgcgttta tcggcacggg cagcagtttt ctcgcttttg
    19081 ctgcgctggc ggcgaaacat cagattgata atcccggtta tgcgcataaa tcgttttatt
    19141 atctgggcgg cttaaccgaa ggcagcgaga cgattttgct gtttgttctg agttgcctct
    19201 ttccggcctg ttttccgtgg tttgcgtggg tatttggtgc gttgtgctgg atgacgacct
    19261 ttacgcgggt gtggagtggg tatctgacgc tgaagtcgct ccagcgtcag taattgcaat
    19321 gttatttact gctgtccggg ccgcgttcac cggttgctac cggatttttt ggatcgctgc
    19381 tccattcgta ccagccaccg tcataaacgg agacgttatt ccagcccatt gcgcgtgcgt
    19441 acataaaggt ttctgacgcg cgccagccgg tgccgcagta gaatgaaact tgctgatctg
    19501 gcttgatatt ccatgctttc cacatagcgg taatatcatc ggcgctacgc atggtgccat
    19561 ccgggttatg gaaatcttcc atatgcgtcg agtcgctacc agcgtgtccc caacgtgctc
    19621 cggctatttc accttttggt ttaatatagc tgtaaccgct ggtcgtaccg ataaattctg
    19681 gccacgaacg aatgctcacc agcgatgcat cctggcgatg cagcagtcca cgcgcttgtt
    19741 caatatcaag catcagttgc ggttgtgccg ggatcttcac gccgaaatcc ggttccgctt
    19801 tcactttcgg tggcgttccg cgctcaacgg gcagtcccgc gtcggaccag gtttgccagc
    19861 cgccgtccag caggcggaca tctttcacgc cagcgtagag cataatttgt gccacacgcg
    19921 ctgcagcgta tacgtcacgc ccgtacagaa tgaccgtggt gtcatggcga atgccgtgtt
    19981 ttgccagcat cgcttttagt tgttcatcag aaactttgtt ccacagcggt tcgctttcca
    20041 cttcgttggt atcgatgtag tcagcgccgg gaatatggct gataaggtaa agcttaggag
    20101 cgccccaggc cgcttcaatg actttccagt caccggcagg tttcgccgta acctctttac
    20161 cttgttgcag gtcgtgcagc cattgcggat aaaccagctg ctcaaaatgc ggcagttttt
    20221 gcagacggga aggttcgctt agcgcgtcac tcagaatgga gatatgcgta aaacctgctt
    20281 tttgcagtcg ctttttgacg gcgtcgacat ctttgtcatt accgtacagc gccaccggag
    20341 catcggcttt caggttatgt tgcttgatcc actcgttgag ctgttcggtg ctcattttgt
    20401 cgagccagtt agcagagagg tttaaggcgg caggttcatg accagaaggg ccatttaagg
    20461 tttgtggcca gccgttataa aacgcgctgg ggcgagtatc tatcgctttg ccattttgtt
    20521 gttgaagctg gtcaagtgta agaggcttcg ccagttcagc ggcccacgaa gaagcgcaag
    20581 ccagccctaa agccattgcc agcgcggtca tttgagaaac acgtttcatc gaaaatcccg
    20641 acattgtagt tttgagtgcg ccattctgaa ctgacgcatt gagaaaaact ttgcgttatc
    20701 gcagtttgtt gtaattttct gaccactgcg ccatatccag aacagaacta tcagcaggaa
    20761 catcctggag atcgtgcgtt acctgaacga cggggatcgc ctgggcgcga acttcgctga
    20821 acacccactg gcgaaaatta tcgcgcagag ccacatctag acggctgaat ggctcatcca
    20881 ggagtaacgc ttttggctgg gcgagaaggg cgcgtagcag agcaacgcgc gctcgctgac
    20941 cgccagacaa agtagcagga tcctgatgga aagctccgtc aaggcctgaa cgctcaagtg
    21001 catcattcac ggcattacgt cgggcattcc ctttaagtgt cgccggtagc gccagcagta
    21061 aattttgccc gacgctgaac tggtcaaata acagtgcatc ctggaaaaga atgccaatct
    21121 gacgctgtgc ggtgggtagc atgtcaatcc gttgctcatt gagccatagc tcccctgtac
    21181 aagaaaactg tccggccagt ccaccaatca tccatgaaaa cagagtggat tttccacagc
    21241 cagacggccc cattaacgtg acaatgtcac ctttatccac cgtaaagtta acgtttgtca
    21301 gcaagcggct ttctggtaaa cgtagcgaaa catttttcac gcagagcatt agcggagtcc
    21361 ttgtctgaca taacctaccc attttgcgac taacgcggtc agggcaaaaa taataagcgg
    21421 taatagcagt tgccataaag cctgggcggc gagaatgccg ttgctgccgc cgctgcttaa
    21481 tgccaccgct tcagtggtga gcgtcggaaa acgccccgcg cccagccaca gcgtcggcat
    21541 atactgtgca atactgactg aaaatcccac cgcgaaggca atcagcgtag ggcgtagcat
    21601 aagcggacat ttcacgtaaa agaagatttt ggaccgcgac cagcccagtg tttgcgcaat
    21661 caaaattaac cgtgaatcaa tgcgctgcca ggcgggttgc aggataaaca gcatccacgg
    21721 cattacccac agcagatacc cccagaccac cgccgtccag cttccatcta ggtttagcca
    21781 tagcgccagc gtgtactggc ccgccaccag tggcagagcg ggtaataaaa tgggcaacca
    21841 tagccagaaa tgccgacgtg acgatcccca ttccagccac gccagtatca ggattaaagc
    21901 gataaacgtc gcggtaagtc ccatcgtcag gctattgatg agcgcttcac tattgatcgt
    21961 cgactgatcc gcgaggatcg ccaacagaac cacacacagc acaccggtca agggtaaaaa
    22021 actcgccaga gtaatgccag gcaataaagg cgtggcaggc ttacgaacgc catctacgcg
    22081 gggaatagta cgcctccagc tacgccatag cagatagccc agcaaaacgt aggcggcgag
    22141 taacagcatc agcagcaggc tggcgagtgc gcctttcgct tgttgatcgg cgtcgccctg
    22201 agttaaccac tgccagctaa ttactgccag cgtcggggga ttacctggcc caaggataat
    22261 cgccacatcc acgaccgaca gcgaccaggc cacaatcgcc agcatcgcca ttgccagcgc
    22321 aggcgcgaca gagggcaaca gcagccagtt caggcattgc cagcggctgt agcccagcga
    22381 atccagcaca atgagttgct gcaacagccg tttttcgctc aatactgccg ctaagatcca
    22441 cagcagaaat gcgctttctt tcactgccag ggtgaggccc agcccaatac ccaatttgtc
    22501 cattggcgga gtgaaatacg ggaaatagtc ataaagcagc cctccgtcag caaagagcag
    22561 aagggcgctg gtggcaaaag ccacatgggg aatggcgagc agccacggca gacgggcgca
    22621 catacgctgc catttcggcc ctggccacag cgctacaatc accagcaggg caatcaacaa
    22681 cgccccgacc gccgcaatgg ttgtcgacac cagcgttgcc agtaatgcct gcggtaactg
    22741 tggatcggca aataacgcct gccagtgcgt caacgacaaa gcaggcgata tcagcgtgag
    22801 aactgccggg atcagcggtg cataaatcac cgccatcatc gcccacagca aaaagattaa
    22861 tgcgtaccgt aacggtgtag ccattcttgt tccagcgcat ttacccaacc tgcgtgcggt
    22921 tcagccagta ccggcggcag atcctgcggc attctcgatt gcaatgattc gcgctgcccg
    22981 tcaggcagtt tttgcggatc gagaacagaa ggatcgcccc agacagcggg atctgcttta
    23041 cgcagttgtg catcgggtga gagcaggaaa ttggcaacta ccttcgccgc agcactggca
    23101 ttcgcgttgg cgggaatggt gacgaaatgg acgttgccaa tcatcccctc gctaaagcca
    23161 aagctgtaac tgctggcggg taaatcgccg ctggcgattt tttgctgcgc atgagcagga
    23221 ttaaaggtca gcgacaggcg caatgtgtta gctttcagca gagcatccat ccgcgcgggc
    23281 gaaggcggga aatcctttcc ttcgcgccat aaatacggat gcagcgcatc aagaaattgc
    23341 cacaagggcg cagtgacacg ggcgaaagtc gcatcgtccg gcgcttcttt taatgcggcg
    23401 ggatcgggtg tcaacataat cagcaactgt tcaagaaacg ccgtgccggt aaagtccggt
    23461 gggcgcggat aggtaaccgt gccgggattg gctttagcaa actccagtaa ggcttgcggc
    23521 gtttgtggtg gctgtggcgt aacatcgcgg cgggcgataa acgtcagttg tgcgccgccc
    23581 cagggcgatt cagccccttc tgtaggcact gaaaaatctt cccgcaccgg aagctgtgtg
    23641 tcgacatagc gccagttggg cagagtctct gcccagtcgg tttgcagtaa attagcctct
    23701 tttaaggtgc ggaagttttc gccgttcacc cagagcagat ccaccgaacc gcctgttttg
    23761 cgtccggcag cagcttcggt ctgaatgcgc ttcaccgcgt ctgcggcatc cgcgagatga
    23821 acaatcttca ggtttatatc gtaatgggtt ttcatctcgc cgctaaccca gtcgagatag
    23881 cggttaattg cggtatcgcc gccccaggcg ttaaaccaga cggtttgccc tttggcgtcg
    23941 tttttgattt cctgccagtc actggcatgt gttgccaaag aaaacagcga taacaatccc
    24001 agcaaccaca cacaatggcg catcatctct ccttaagaac gtttttcagg aatacggcac
    24061 agcccctgag cgggaatgtc catggcagaa ttaaaacggg ctgacaggtt ttgcagtccg
    24121 atcaatgccg tcagttcggt gagcgcctga gcgtcaaaat gcgtaaccag tcgggcgcgc
    24181 agggcatcat cgaccgttgg cggcgttacg cttgcggctt ctgcgtactc cagcgccagc
    24241 cgttcttcat cgctaaagag cgggctttgc cgccagtcag ccactgccag cagtttatcg
    24301 gtgctgccag tgcgctcggc gactttcata ctggtgatat ccacgcaaaa ctcacacagg
    24361 cacatttgcg caatgcgcgc gctgacaagc gatcgcacta ccggatccag cggtgagcgt
    24421 ttgcgctcca gccagccgac aaatatcgac accagataaa agataaacgg gatccgcccc
    24481 caccagcgaa tcgggcttag cacctcgccg taatggcgac gatgcagcca cgcctgcgga
    24541 cgtaaaatga aaggaatttt gctaagcggc ggtaaaccca tcaggcattc ctcttctggc
    24601 gctcaagcca tattttcttc gccataaaaa tcaccacggt cagggcaaac agaataaaca
    24661 gtccggtgac aaaccagaaa gtaccgcctg ttaacatgct gcccgcccag gaataaacaa
    24721 tagtcgctgg taactgacca agcccggtgg cgataaaaaa cgagcgaaaa cgtattgaag
    24781 tcaaaccggc agcatagctt acgggatcaa aaggcacaaa aggcaataat cggcatacca
    24841 gaatggtgtg tttaccatag cgggtaaaaa agccgtccat actattgagt accgttttac
    24901 ccgttaactt ttccaccact tcgcggccca tcactctggc gataaaaaag cacagcgccg
    24961 cgccggccat cgaactggtc cacgacagca agccgcccca gaacgcgcca aacagcgacg
    25021 cattggcaaa ggtgatcaaa aacgcaggta gcggtgcagc aatggcctgt aaaatcatca
    25081 acaagaacga gacaactgcc gccagtgcgc cgtaagactg aataaagcgt tctataccct
    25141 gttggtccac ggcggcaaac gccgcaacgc tgcgattgat aaactcatgg acgccgggaa
    25201 gcagcgccca tgccagcagc atagcgaaca acaggatgat gagggttata cggtagtacc
    25261 agatttttcg cgattgcatc ttcatcatcc tttatcctgc tttattcggt gattttaaag
    25321 gtaaccgttg ccagcgtgtt atccgccggg agaaccgagg cattgccttt gaatttaact
    25381 tcacctcgtt tttcaaccgc accataagtg tatgttgcat tgctgacgat gccgaccggg
    25441 cagctatcca gacacaccag gcagcccgtt ttcttctctt ctgctgccgt taaattaccg
    25501 ccaaagcgca tgtccagttt ctttccatta ctgtcaacaa tcacttcatc gaaggaatac
    25561 gcttttgccg ccccttgcca gttgaccgaa atatccagtt tgctgcctgt gacatgagtc
    25621 gtttctttat tatccatcgt catgttttcg cccggtgtac cacctgcctc tttcagggct
    25681 tcataaaagg ctttcggggt cgcataaccc ataaacagcg atttatgtcc gttggagcca
    25741 tctttaaaca caataccgtg acgagtgtcg tcggtgagga aacgaccatt aatttgcacc
    25801 agcatggtta ccgtatggtt attggcatcg acacgcagcg gttggctgtt cgaaagtccg
    25861 tcgtattcca cttttgcagc cgcattctct ttttgatcgc agccgctaag cccggcaaca
    25921 gccagtaagc agagtgcagc cagtcctttt ttccatgaca ctgaataatg ttgcaacatc
    25981 gttctatcct tcatttttgg ggtgagaaag tgggctgcgg cgcgaagcag acagatccac
    26041 atgtttgtgt cgggcgtaga gttttgcgag ctggacgaga ataaacagtg ccaggcccgc
    26101 cagacagagt tgtaaaataa agcgcagcgt aatgccttca ttggcgagat cgcttgccat
    26161 cacggtataa ataacaatac cgggcagggt tgtgagtgcc gaaataaggg tataaggcca
    26221 gaaggcgatt gtggttaatc cgtaagcgta attttgaata ttgtaaggaa acaacgggat
    26281 taagcgggtc agaataagaa aatcaatacc gttacgcgca atgccttttt caatggcctg
    26341 aaaggtatgg ctatggccaa cgtatttcag cagtaaatca cgtcccagcc agcgcgccag
    26401 caggaatgag cacgaggagg ccagcgtggc ggcaattaat gatagtagtg tccctaagag
    26461 cgggccaaaa actattcctc cagcgatcac cagtatgctt cctggtaaca gaaagagggt
    26521 ggcaatgatg aataacagaa tatagaggct ataaccgaac aatccgctct ggcgaatgag
    26581 tgtcagtaag tgaggtaaat cggtgagcag ctcgaataaa ccgaaagcgt ggaaagcgta
    26641 aatgaccagc gcaaaaataa gacaggcaaa aagaaattta cgctcagcgt tcatcgtgga
    26701 gtttcctata tcaaacttga taatagagaa gtggtcagac aactaaaaac gcagtgacgg
    26761 tataacaaag gacggcaggc aacaaatcat tactgacagt ttgttgattt ttctcaaggt
    26821 taattctcct gacccagttt gagccaggag aacagctaaa ttagcggcgg aaggtcgccc
    26881 agacgggggc gtgatcggac ggtttttcca tgctgcggat ttcatagtcg atgccggttt
    26941 ctacgcaaca ttctgccagc ggttggctgg cgagaagcag gtcgatgcgc agaccacggt
    27001 tatcgtcaaa accttttgag cggtaatcaa accatgagaa acgattggct gtttgcggat
    27061 tcgcatggcg gaaggtatca accaatcccc agctcatcag tctctccatc cattcgcgtt
    27121 cctccggaag gaaagaacat tttccggtac gcagccagcg cttacggttc tcttcgccaa
    27181 tgccaatatc caggtcgcca ggactgatat tcatatcgcc cataatcagc accggatttt
    27241 cacgcttgag ttcggtttcc agatagtttt gcagattctg ataaaactgc gcctttgccg
    27301 ggaattttat cggatggtcg cggctttcac cctgcgggaa gtaaccgttg atcacggtga
    27361 cattgcccag cggcgagggg atttccgcca taataatccg ccgttgcgcc tcttcgtcgt
    27421 caccgggaaa gccgcgacgc acggcaatcg gcgtctcttt ggtcagcagc gccacgccat
    27481 aatggccttt ctgtccgtga taaaacacgt tgtagccgag cttcgccacc tcttcgagcg
    27541 gaaacatatc gtcatgaact tttgtctcct gcaggccaat cacatccggt tggtgctttt
    27601 cgacgatggc ttcaagctgg tgaggtctgg cgcgcaggcc gttgatatta aaagagacaa
    27661 atttcatagt cgctgccatt tattcagata aagagtgcgt ggatggtagc agaatttcgc
    27721 tttgcgtacc gcctgttgtt aagtgatttg atcgatggtt gttaatgatg tcaacgatgg
    27781 cgcaaaaaat gcccgctttt ggtgcgcgtt gcgtcagaat ggcgcagtaa tttccagtaa
    27841 atttcgtcaa tgatcacaat tccagaatta tattttactt ctgcataaca ttgcgttttt
    27901 tattcttttt attcacccac atcgc
//
LOCUS       DAZKGF010000049        27893 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZKGF010000049 DAZKGF010000000
VERSION     DAZKGF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.049"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..523
                     /gene="ssb"
                     /locus_tag="ACHOQV_002017"
     CDS             <1..523
                     /gene="ssb"
                     /locus_tag="ACHOQV_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9630393.1"
                     /translation="HLGQDPEIRYFPNGGGAVANLTLATTDTWRDKQSGEMKEHTEWH
                     RVVIYGKLAEIAGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYSTEVVVSVNGTLQML
                     GSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGMPPMDFDDDIPFMG
                     RGYGVERRIIHVI"
     gene            572..1492
                     /locus_tag="ACHOQV_002018"
     CDS             572..1492
                     /locus_tag="ACHOQV_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP9630394.1"
                     /translation="MNENTPTTPTPHDAAFRAMMECPSVARDFLKGALPPEQLNRCDM
                     NTLQLEPTTFVDPDLRQFACDVLWSMKTTDGRDGYIYTLTEHQSKADKYMALRMMRYV
                     LAVIHRHLKAHACAPVVIPVLFYHGVPSPYPYSMNWPDCLDDPAFGRELYGEGRPPRV
                     IDVGLLDDEGIRRYKQMAALMLMMKLRQKKVDLMVHLDFLSQLIQIQETHTQVVTLFN
                     YMVKECDSASPEFIRAMAERLPRHEDDLMTIAERLELAGIEKGRQEEREKALQENYAS
                     ARRMQQMGISSEKIKQILQFSDDDLRNALR"
     gene            1678..2277
                     /locus_tag="ACHOQV_002019"
     CDS             1678..2277
                     /locus_tag="ACHOQV_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFP9630395.1"
                     /translation="MKKRFILSGLTVCLLMAGCQGTQKLQQRDAAMPVPSVTVNRNVQ
                     PASPDVYSQAPEVVRYDRYLLVSTEPQAVQRDPLSQVIDIRIPSSLKPTVADAMRYAL
                     KQSGYTLCATGPANGVLYRQPLPAVQYQPGPLRLRTALQLMAGPAWQLEVDDVQRVVC
                     HSLRDGYQLPVSQLAPVPVTVPFVGREPGVKATGGFLKK"
     gene            2274..3023
                     /locus_tag="ACHOQV_002020"
     CDS             2274..3023
                     /locus_tag="ACHOQV_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630396.1"
                     /translation="MSEKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYG
                     VLMLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVL
                     TASWKEQLENLPEMLEQIESAREKLSLRLDTLSGLVEKMQKVPESPSQPDSRKAGMKK
                     TEEKKPAPVTSSRKSRAVHTVRQAPFVLTGTELRGGSTRAAVAPRGYTSLSQVALLGE
                     GESVAGWTLVYAGHGEATFRVNGRQTVLRVE"
     gene            3027..3746
                     /locus_tag="ACHOQV_002021"
     CDS             3027..3746
                     /locus_tag="ACHOQV_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFP9630397.1"
                     /translation="MKRKILYLLALCPVLAQSAGQTNMSSEAQRTTGTVVTQSTTSGL
                     QQQAGQWGVSEEDYRRYQSLMSGPRGIQSPGLDPLTVLGIEARTPAERRVWAEKWVRA
                     EYERTEKELAFQQEVNAAWKRLYPGILPVSMGKTGALTGDTGGRLALFVKAKECGTCD
                     ARLASVLATGRQVDIYLVDSQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRF
                     GEGKMPVVLQQGADGWRVAAF"
     gene            3725..4405
                     /locus_tag="ACHOQV_002022"
     CDS             3725..4405
                     /locus_tag="ACHOQV_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFP9630398.1"
                     /translation="MARGGILTVLLMVACSGVCRAAVQNVPDGYVRVARAHGIPAELL
                     YAVSLTETSMSPEAMSGVLKQRRRDFRQPDVARPWPWTINVAGKGYRYATRLEAWQAL
                     QVFLKHYPAKRIDVGIAQVNLGWNGHRFPSTWAAFDPYTSLNTAAVILQECRRRHPVS
                     WLQVAGCYHHPAGGAPAARYTAVVRRHLARLHRDNFLNPSDVHVAATAMLTPSPRYIW
                     TEPQEEQK"
     gene            4402..4920
                     /locus_tag="ACHOQV_002023"
     CDS             4402..4920
                     /locus_tag="ACHOQV_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFP9630399.1"
                     /translation="MKRERQFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASVVPSPSFPQGEAAMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTW
                     LQMNAAVLRERKAAGMIVNVADESSLRVLRELAPGVVMVPASGSVLARRLQISHYPVL
                     ITDNGLSQVPAP"
     gene            4917..5510
                     /locus_tag="ACHOQV_002024"
     CDS             4917..5510
                     /locus_tag="ACHOQV_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9630400.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKASV
                     RTRRHQRYRATASRVMKRLNELQGDARRLKYLRKISPYVFEELLLLAFEQQGMPVIRN
                     PSYSGDGGLDGQVVIGGECWLIQAKRYGRTIRPAHIDAFGELLRRKGCRGFFIHTGRT
                     GNISRARLTLWPDIELISGQRLLQLLNGTLLSNKGES"
     gene            5507..5989
                     /locus_tag="ACHOQV_002025"
     CDS             5507..5989
                     /locus_tag="ACHOQV_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630401.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLSGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCPSVISVSGMYVDEQECPRTAISFGEAVAEDIRELK
                     MQAVVLLLGALFMEGVSSVILFRGCGSAAVSGEGRGGPAVMPWPDGIGISKVQERRAE
                     "
     gene            5986..6774
                     /locus_tag="ACHOQV_002026"
     CDS             5986..6774
                     /locus_tag="ACHOQV_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630402.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSWLELLERGKRMDINIPLLEKNPGYYTQAEQQHAGMGFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEGRTQLHNVAQSIYHTDQVFRSACREIHNL
                     AEPLSRHDVYLRLQIRRQCVSREDLACWKVDRFRTEAQLTVDDVHAGGHDGPLVYKAL
                     LLNAADAWREVIVLQRRLEALSASPEKALPRSWWRRLLQRGTRS"
     gene            6771..8867
                     /gene="traD"
                     /locus_tag="ACHOQV_002027"
     CDS             6771..8867
                     /gene="traD"
                     /locus_tag="ACHOQV_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFP9630403.1"
                     /translation="MSNRYVIEALLRPAVELNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAVVRFRQGMKIIRYRRNLRRLPRYVMSSRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGSLYRTARWLEMKTERSLPWIGQLLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGDVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIVARALVALGERPDYTLIMRYVNNIADLYIRYAGKVI
                     SEQMPELENAILNNMNVLGEADVPRTMQNQPDAVRIWAIEVALSSEAGKKLYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGKTAELLAPDYLDMSDTRPVFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMNAGLPGGNGTKNPVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARTGSAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLSQVEIYTKTLVSGHQDTADVTTGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPATDADDALMPESIAKIAEAMRRNYHTGEGW
                     WNETPSVNMPEGEQNG"
     gene            8860..9621
                     /locus_tag="ACHOQV_002028"
     CDS             8860..9621
                     /locus_tag="ACHOQV_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFP9630404.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSTPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVGRWLSHYLYEYLMATVYVTVVTLIRV
                     TILVLSVPLFVMVILIAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCILY
                     LSWPTSVYPNLLIWPAAMLLGGAITVTMASFKKYL"
     gene            complement(10005..10571)
                     /locus_tag="ACHOQV_002029"
     CDS             complement(10005..10571)
                     /locus_tag="ACHOQV_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630405.1"
                     /translation="MDEGKQPGTTGMNDIISIIILLTVYLIGLIFIILTINDIDRSSK
                     KRKYEKKLKEAIINGSLQNNDICLLAERWSASHEHIKSALLSVFNDFLTDNNFSHHVP
                     RVRELITWYEKYAPFTELPEDIKQQLQNIISLPDVSESEVQLLATSLHDIYNSCQKKE
                     KLARRINYVLGAVSIASFCISLFQILFK"
     gene            10684..11025
                     /locus_tag="ACHOQV_002030"
     CDS             10684..11025
                     /locus_tag="ACHOQV_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFP9630406.1"
                     /translation="MFSFPASSCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGERYFFDYLRARADLQTIRDGIDHYLAPSRAQPRSSA
                     AVTGDYRRERP"
     gene            11025..11267
                     /locus_tag="ACHOQV_002031"
     CDS             11025..11267
                     /locus_tag="ACHOQV_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFP9630407.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
     gene            11344..11658
                     /locus_tag="ACHOQV_002032"
     CDS             11344..11658
                     /locus_tag="ACHOQV_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFP9630408.1"
                     /translation="MAVFLCTGITPALADLPQIEPPTSGGGGGLFGQIKGYLQDGVVL
                     FGLVLSAVAFVKVAEAALSTFSEVRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASI
                     IL"
     gene            11671..12033
                     /locus_tag="ACHOQV_002033"
     CDS             11671..12033
                     /locus_tag="ACHOQV_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFP9630409.1"
                     /translation="MQTIRFLPDRLNVEPAVFRGFTTPELGLAALTGAVAGGIWPLPL
                     IPLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTF
                     IREAHGWSLRRTRSRGKK"
     gene            12030..12692
                     /locus_tag="ACHOQV_002034"
     CDS             12030..12692
                     /locus_tag="ACHOQV_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFP9630410.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQRLEAASPAAGKGD
                     NQ"
     gene            12692..13600
                     /locus_tag="ACHOQV_002035"
     CDS             12692..13600
                     /locus_tag="ACHOQV_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFP9630411.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTAASARSAEHKKVVPGGDDGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNTHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEPASRRIPSRGEVKHAD"
     gene            13590..15077
                     /locus_tag="ACHOQV_002036"
     CDS             13590..15077
                     /locus_tag="ACHOQV_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFP9630412.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPTDQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQVKGYSNEQQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTD
                     PYPFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVR
                     TLPSSDVNGSSNAQAKNNSSTGGIGWISDDDGIPCISGTRKSNASTYLPTIFALSAAG
                     AAGDALSEGQYTSQNNVNGITSTLTGSAGEAALGKALSGGMNETAEWVKQRYGQTFDA
                     IYVPPGQTLAVHITRQLAIDYEDQGRRVRYDFTLPGEVHGTGGLD"
     gene            15113..15505
                     /locus_tag="ACHOQV_002037"
     CDS             15113..15505
                     /locus_tag="ACHOQV_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFP9630413.1"
                     /translation="MTVMVAVFILPGCSTSKEELLPPGEQSMLEIWQGKGSGGHSRHM
                     TAARDTLRRPLTMPEQQADAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGT
                     SPVPGYSTVFPFYSRTQYAMPGERTEAL"
     gene            15643..18339
                     /locus_tag="ACHOQV_002038"
     CDS             15643..18339
                     /locus_tag="ACHOQV_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFP9630414.1"
                     /translation="MTRQDETKVYHACPSVIDFLPWVEYLDEEQCLLLDDGVSVGAVY
                     EVSPAATEGRTAERLEQIRDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLR
                     GYVKPHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRW
                     IGKNNHDPMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDR
                     DMLYRMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEK
                     LRTPPEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAV
                     GENSESLRAREDVATVKKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHLAGLLPVTGRETGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCGSLSQIMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVKPGTGVCLPPFADAHQLVEQGETLQSVDEH
                     SLPDLDEDEGDEEEEKRDILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMAT
                     HTTYREGRQMLPVDLQSALWEISRDTQRNDVRRAKAAEMAESLGMFTQPGSFEAELFN
                     REGKLWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDILFVVD
                     EAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCLTM
                     PPEEVNNIARFKSLTEEQKAVLLSASKLSGCYTEGVVLAKRTEALFRVVPPSLYLALG
                     MTEKEEKAERRALMKVHQCSELEAAFHVARKLDGLRGLAGRE"
     gene            18344..18721
                     /locus_tag="ACHOQV_002039"
     CDS             18344..18721
                     /locus_tag="ACHOQV_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9630415.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
     gene            19024..19212
                     /locus_tag="ACHOQV_002040"
     CDS             19024..19212
                     /locus_tag="ACHOQV_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP9630416.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDEKMTQDECEKLFAPAKEP
                     GRYIGVRVEDFRCEVVKA"
     gene            19597..20046
                     /locus_tag="ACHOQV_002041"
     CDS             19597..20046
                     /locus_tag="ACHOQV_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630417.1"
                     /translation="MLKKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQLS
                     DVKDAAEEVKMQAAGKVAEVSEAAQSGLSEVKDAVEEVKTQAAGKMAEVSEAAQSGLS
                     EVKDAVEEVKTQAAGKVAEVSEATQSGLSDVKDTAPEKGAEISVTEE"
     gene            20238..20870
                     /locus_tag="ACHOQV_002042"
     CDS             20238..20870
                     /locus_tag="ACHOQV_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9630418.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTPLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSPVYFRDAVAFSSLALFEEHPDFHALLTG
                     CIRSCTVRVKNIISADLSRPWMLGEVASLLYISESLLKKKLQEELTTFSELLLKERMQ
                     YAVQLLSAGENRISRVAELCGYASASYFISVFRRYYGITPNQWLPVMQPD"
     gene            21006..21401
                     /locus_tag="ACHOQV_002043"
     CDS             21006..21401
                     /locus_tag="ACHOQV_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFP9630419.1"
                     /translation="MYRLLLYSFLILPVAASAGTTIYTDSHQRPMNPPAGVRVVLLDA
                     PEQTQDTFFGVLPAEPAEASARVMERMQSPEWQSFQAGLAEHYRALAHAWSLGLKKYP
                     AVVFDDSEVVYGTTDVVLAEQLRTGGGQP"
     gene            21398..22372
                     /locus_tag="ACHOQV_002044"
     CDS             21398..22372
                     /locus_tag="ACHOQV_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFP9630420.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQNCISW
                     RVSGICYWLFCTKFSCKVRTSVKVSHYIPEVVVSTYTAPGGNPWREMSAVSSTAGGPE
                     NAISKGIAGLSAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIGGMVPGYSCESP
                     VTPFVPYFLSTLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWGNVYPRSGFVTQ
                     TDDDKAAAVVAQRVADIITRTGEPHVYQPVKAGKKDGYWPPDPVEENTGTRNHKWQRL
                     TPSVSTSCAVFPDGEHEAAETGNAAFALWQPYSCCKKRGQTFLGSTNF"
     gene            22386..23807
                     /locus_tag="ACHOQV_002045"
     CDS             22386..23807
                     /locus_tag="ACHOQV_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFP9630421.1"
                     /translation="MKQKMTIPVLAVVLCAGTVQADGNFLSLPKVNDSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGEEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEATRVNTELLAGMVNGRDKPT
                     AGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELAMADTVETALLMRRMLLTG
                     MAEPYAAAQPAALEEGERRIEALDREIMALKNEMELKRDLARNSVLTIIERDNERVGN
                     NPMIQQADNADSRIHSLDAPDKE"
     gene            23812..24153
                     /locus_tag="ACHOQV_002046"
     CDS             23812..24153
                     /locus_tag="ACHOQV_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630422.1"
                     /translation="MSRIYGRMKKRMKVFALYTLLLTGITVFSLWLADTGIRHAEGFA
                     ALREWMYETRYGWLGWRLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILL
                     CEYAIFAEPGR"
     gene            24160..25668
                     /locus_tag="ACHOQV_002047"
     CDS             24160..25668
                     /locus_tag="ACHOQV_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFP9630423.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPEEPGETGYSGVISSIGDQTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEIQRTFIENRALADELQDFTNDCYSLALYMWKQRDQGQSKDKKVLDDISWIGSATFM
                     QGDYGTLQSKMPRAAFPWNTARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVMEGVSD
                     HYWLRLSAAMKMIGSSQVDYKEEVIKRLVSPANLTLSQGGEVYRGYGGNADMTTMNAV
                     ARIAGTAGSAVAALGAFPALDAMRQALPMVQSIILMAIYIMIPLILAFAAYELSTLIT
                     LTFVVFAVHFLTFWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVMGT
                     MFLVLPAVWLGALSWAGVHAGVIASGIQRGTETVSGAAQTAGTIARSGVKSK"
     gene            complement(25731..25973)
                     /locus_tag="ACHOQV_002048"
     CDS             complement(25731..25973)
                     /locus_tag="ACHOQV_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630424.1"
                     /translation="MNNESEKAMWRCKPVDNPWTFSSFAKKVICSVLPDTQPSLDAFV
                     NSTNSEEHLNFSSDSTFPSCPDINNINNINNINNYY"
     gene            complement(26168..26659)
                     /locus_tag="ACHOQV_002049"
     CDS             complement(26168..26659)
                     /locus_tag="ACHOQV_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP9630425.1"
                     /translation="MPIPPYMWLKDDGGADIKGSVDVQDREGSIEIIGMSHGINLPVD
                     NANGKITGTRQHSAMMIEKEVDSSTPYLYKAAATGQTLKSAEIRFYHINDAGQEVCYY
                     TVLMENVKVTGVNCGVPNVKLAGNDKMNHVESVSLQYEKITWRIVDGNIQYTDSWNER
                     PTA"
     gene            complement(26661..26924)
                     /locus_tag="ACHOQV_002050"
     CDS             complement(26661..26924)
                     /locus_tag="ACHOQV_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016534744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630426.1"
                     /translation="MVQTIARKIAKTVFFFIIMFFVARSLSKPEDYVNHDLVDKLAQF
                     ISGDINAESVYDAYFYIDISVVVVITTTIYMVIMKIISKKRNK"
     gene            complement(26902..27420)
                     /locus_tag="ACHOQV_002051"
     CDS             complement(26902..27420)
                     /locus_tag="ACHOQV_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016192010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2778 domain-containing protein"
                     /protein_id="HFP9630427.1"
                     /translation="MALQGKFILNGADYAPFNLYGVGVFMAFSGNGIYRNKGACGAIK
                     GDGPLPPGKYWIVERGSGGFFSKRKAEIQDTWNKIRYGAEFGRNEWFALYKDDWGIDD
                     GTWINGVYRGLFRLHPGTISEGCITIPHNSDYALIRDALLRTTPVQVPCMRSLMARGW
                     IEVIANGSNNCP"
BASE COUNT         6334 a         6415 c         8572 g         6572 t
ORIGIN      
        1 tcatctggga caggatccgg aaatccgtta tttccccaac ggcggaggtg cagtggcaaa
       61 tctgacgctg gccaccactg atacctggcg tgataaacag agcggtgaaa tgaaggaaca
      121 cactgaatgg caccgtgttg tgatttacgg aaaactggct gagattgccg gtgaatacct
      181 tcgtaaaggt tcacaggtgt atattgaagg tcagttaaga acccggaagt ggttcgatca
      241 gcagaatggt gttgatcgtt attccacgga agttgttgtg agtgtcaatg gtaccctgca
      301 gatgctgggg tctccgcgtc agcagcaggg tggtggaaat gtttcatctg ctcctgctca
      361 gggaggatgg ggacaaccac cacaaaatgc acaacaatca acgggaaaaa acggtcaggc
      421 ttcctcagga agcgggggaa tgccgccgat ggactttgac gatgatatcc cctttatggg
      481 gcgtgggtat ggcgttgaac gacggattat ccatgtcatc tgatgttaac cacggggctt
      541 accgccccac ttttaattca aggaggcggt tgtgaatgaa aacacaccca ctacccccac
      601 gccacatgat gcggcgttca gagcgatgat ggaatgtccc tctgttgcga gggatttcct
      661 gaaaggcgcg ttgcccccgg aacaactgaa ccggtgcgat atgaacaccc tgcaactgga
      721 gccgaccacg tttgtggatc cggatcttcg ccagtttgcc tgtgatgttc tgtggagtat
      781 gaaaaccaca gatggcaggg atggatacat ttatacgctg acagagcacc agagcaaggc
      841 ggataagtat atggccctgc gaatgatgcg ctatgttctg gcggtgatac atcgccacct
      901 gaaagcacat gcgtgtgctc ccgttgtgat accggtgctt ttttaccatg gtgtacccag
      961 tccttatcca tacagcatga actggcctga ctgcctggat gatccggcgt ttggtcggga
     1021 gctttatgga gaaggcaggc cacccagggt gattgatgtc ggtcttcttg atgatgaggg
     1081 tatccgtcgc tataagcaga tggctgcgct gatgcttatg atgaagctcc ggcagaagaa
     1141 agtggatctg atggttcatc ttgacttttt gtcacagttg attcagatac aggaaacaca
     1201 cactcaggtg gtgacattgt ttaactatat ggtgaaagaa tgtgattcag cttcacctga
     1261 atttatacgg gcgatggcag aacgtctgcc aagacatgag gatgatctaa tgactatcgc
     1321 tgaacgactg gaactggctg gtatcgagaa gggccgccag gaggagagag aaaaggctct
     1381 tcaggagaat tacgcatcgg cccgtcgcat gcagcagatg ggtatatcgt cagagaaaat
     1441 caaacaaata ctgcagttct cggatgatga tttgcgtaat gctctccggt aatcggtaat
     1501 ttatgttaac cgcaggattg tgacatattt gttatgttgc tttccttcct gtcaacaaag
     1561 cgcctgcatg tctgcaggcg ttttttttcg ccagcgggta atgagttgtt cattgagcca
     1621 gccccgtctt cttctgcaca ttgacctctg atttatcact gcagaggtaa atacccgatg
     1681 aaaaaacgct ttatcctgtc cggactgaca gtctgcctcc tgatggcggg atgtcagggc
     1741 actcagaaac tacagcaacg ggatgcggcc atgccggtac cgtcggtcac agtaaaccgt
     1801 aatgtacagc ctgccagccc ggatgtgtat tcacaggcac cggaggtggt gcgctatgac
     1861 cgttacctgc tggtcagcac agagccgcag gctgtacagc gtgatccact ttctcaggtg
     1921 atcgacattc gcattccctc ctccctgaaa cccacggtgg ccgatgccat gcgttatgcc
     1981 ctgaaacagt ccggttacac gctctgcgcc accggaccgg ccaacggcgt gctgtaccgt
     2041 cagcctctgc cggcggtgca gtatcagcca ggaccactga gactgcgtac tgccctgcag
     2101 ttaatggcgg ggccggcatg gcaactggaa gtggatgatg tgcagagggt ggtctgccac
     2161 agtctgcgtg acggttacca gttaccggtc agccagctgg ctcctgttcc ggtcaccgtt
     2221 ccttttgtgg gtcgtgaacc gggtgtgaag gcaacaggag ggtttctgaa aaaatgagtg
     2281 agaaacagcc gcagataccg gagggtgcgt ctggtatgtc gccggagcga agaaaaagtc
     2341 gtttccccgg cgtgttgata ctgtctgtca ctgtggggct ggtcatgggg gcagtggtat
     2401 atggcgtact gatgcttatg accggtcttg acagccggat cacgaccctg gaaggccaga
     2461 gaagagaaat gctttctgcg tcagaactcg cgcccctgag aatgaaactg acagagacgg
     2521 agaagcaact gaaggatatg gaaaagcgcc tggcagtcct ggagcagcag cccgtgctga
     2581 cagcatcctg gaaggaacaa ctggagaatc tgccggagat gctggagcag attgagtctg
     2641 cccgggaaaa gctttccctg agactggata cactcagcgg tctggtggag aagatgcaga
     2701 aagtaccgga gtcaccgtca cagccggaca gcaggaaagc ggggatgaaa aaaacggagg
     2761 agaaaaagcc tgctccggtg acgtcctccc gtaaatcccg cgcagttcat acagttcggc
     2821 aggctccttt tgtgctgacc gggacagagc tgcgcggtgg cagtacccgt gcagccgtgg
     2881 ctccccgggg gtataccagc ctctctcagg tggctctgct gggtgaagga gagagtgtgg
     2941 ccgggtggac gcttgtgtat gccggccatg gtgaagcaac gttccgggtg aatggtcgtc
     3001 agacggtgct cagggtggag tagatgatga agcgaaaaat actgtatctg ctggcactgt
     3061 gccccgttct ggctcagtcc gccgggcaga cgaatatgtc gtcagaggcg caacggacga
     3121 ccggtacggt ggtgacgcag agtaccacat ccggacttca gcagcaggcc gggcagtggg
     3181 gtgtgagtga ggaggattac cggcgttatc agtcactgat gtccggcccc cgggggatac
     3241 agtctcccgg acttgatccg ctgacggttc tcggtataga ggccagaacg ccggcagagc
     3301 gacgtgtctg ggccgaaaag tgggtcaggg ctgaatatga acgaacggag aaggagctgg
     3361 cgtttcagca ggaggtgaat gcggcctgga agcgcctgta tcccggtatc ttgcctgtca
     3421 gtatgggaaa aacaggagca cttaccggag atacgggggg acggctggcg ctgtttgtga
     3481 aggcgaagga atgtggcacc tgtgatgccc gtcttgcctc cgtactggcc acgggacggc
     3541 aggttgatat ctaccttgtc gacagtcagg gaaaggatga gattctgcgt cagtgggcgc
     3601 atgcgcacag catacctgtg gagaaagttc gcagccgtca tatcaccctg aatcatgatg
     3661 ccggacgctg gctgcgtttt ggtgaaggaa aaatgccggt ggtactgcag cagggggctg
     3721 acggatggcg cgtggcggca ttctgacggt tctgctgatg gtggcctgca gtggtgtctg
     3781 tcgtgctgcc gttcagaacg taccggacgg gtacgtgcgg gtggcccgtg cccatggtat
     3841 tcccgcagag cttttgtatg ccgtttccct gacggaaacg tccatgtctc cggaagcgat
     3901 gtccggagta ctgaaacaac ggcgtcgtga ttttcgtcag ccggacgtgg cccgaccctg
     3961 gccctggaca ataaatgtgg caggaaaagg gtaccgttat gccacacgtc tggaggcctg
     4021 gcaggcattg caggtgtttc tgaaacatta tccggcaaaa cgtattgatg tcgggatagc
     4081 acaggtcaat ctgggctgga atggacatcg tttcccgtcc acatgggcgg catttgatcc
     4141 ttacaccagt ctgaatacgg cagcggtcat tctgcaggaa tgccggcgtc gccatccggt
     4201 cagctggctg caggtggccg ggtgttatca ccacccggca ggtggtgcgc cggccgcacg
     4261 ttataccgcc gtggtcagac ggcatcttgc ccggctccac cgggataatt tcctgaatcc
     4321 gtctgatgtg catgtggcag cgaccgccat gctgacacca tccccccgtt atatctggac
     4381 tgaaccacag gaggagcaga aatgaaacga gaaagacagt tttatgtact gagtctgctg
     4441 ctgttctgta caacagctgg cgcagaactg aaagtgattg cagacctggg cggcagggat
     4501 gccagcccct tttttgaggc gataaaccgg cagccaccgg ttcccgcttc agttgtgcca
     4561 tcaccctctt ttccgcaggg agaggcggca atgttaccgg tcagtacgcc tgaaatgtca
     4621 cccggagagg ttgtggcgcg ccggcttcag ttaccgggca tcggtgcact ttttcttgtg
     4681 ggcgatgatg cccattcccg cacatggctg cagatgaatg cagcagtact cagggagcgg
     4741 aaggcggcgg gaatgatagt gaatgtggcg gatgagagca gtctgcgcgt actgcgtgag
     4801 ctggcaccgg gggtggtgat ggttccggca tccggttctg tactggcccg caggttacag
     4861 ataagtcatt acccggtgct gataacggac aacggcctgt cacaggtacc tgcaccatga
     4921 tgccgctgtc tgccacttct gctgcgctct ctgcttctcc ggtactgaca gcctgcctgg
     4981 ttctcagcct gctggctgtt gtcctggggc cggcactgtg gcgcagccgg cgaaaaaaag
     5041 cgagtgtgag aacacgccgg catcagcgtt atcgtgccac ggcctcacgg gtaatgaagc
     5101 ggctgaacga attgcagggt gatgcccggc ggctgaaata tctgcgcaaa atcagtccgt
     5161 atgtgtttga ggaactgttg ctgctggctt ttgaacaaca gggaatgcct gtgatccgta
     5221 acccctcata cagcggcgat ggtggtcttg atggtcaggt ggtgattggc ggtgagtgct
     5281 ggctgataca ggccaaacgt tacggaagaa ccattcgtcc cgcacacatt gacgcgtttg
     5341 gcgaactgct gcgccggaag ggatgcaggg gattttttat ccatacgggt cgtacgggga
     5401 acatcagccg ggcacggctg acactgtggc cggatattga gctgatcagt ggacaacgtc
     5461 tgttgcaact gctgaatggc accttactga gtaacaaagg agaatcatga ttatgacact
     5521 gtaccggttt gttatgctgc tgctccggct tctgattgtc tggccgtcaa tgttggcagg
     5581 atgtctggta atgctgtatg tctggaacaa tctgagtggt ggtgaggcag gcatgacaca
     5641 gcgttatgcg acggagtcat cccgctggcg tgatgcgcct gaagggcatg tgatggtaca
     5701 aaaatgtccc tcagtcatat ccgtctcagg tatgtatgtc gatgagcagg aatgtccgcg
     5761 aacagcgatt tctttcgggg aggctgttgc tgaagatatt cgtgaactga aaatgcaggc
     5821 agtggttctg ttgctgggcg ctttgtttat ggaaggtgtc tcgtcagtca ttctgtttcg
     5881 tggttgtggt tctgcagccg tatccgggga aggcaggggg ggcccggctg ttatgccgtg
     5941 gcctgacggt atcggtatct caaaagtgca ggagagaaga gctgaatgaa gggggcattt
     6001 acagagcgga tacaggcgaa tgctccggcc tgggcacatg aacaactggt tgcatgggat
     6061 accgcagagt ttgcggcaat gtcggatttt attacggaac actactggac gggacagggc
     6121 agtatcaatg tgtaccgcat tgtggggact gaccatccac agtatgccgg aatgagctgg
     6181 ctggagctgc tggagcgtgg taaacgcatg gacatcaata tcccgttact tgaaaagaat
     6241 ccgggatatt acacgcaggc agagcaacag catgccggga tgggttttgt cagcacggat
     6301 ggcattcact ggtatgtcag tgctgacggt aatcaccgca gctgtctggc ccgctttctg
     6361 ttccacctgc agggtgaggg gcgcacacag cttcacaatg tggcccagag tatttatcac
     6421 acagaccagg tgttccggtc tgcctgtcgg gaaattcaca acctggcaga accgttgtcc
     6481 cggcatgatg tgtatcttcg tctgcagatc cggcggcagt gtgtttcccg cgaagacctg
     6541 gcctgctgga aagtggatcg tttcagaaca gaggcgcagt tgacagtgga tgatgtgcat
     6601 gccggcggac atgacgggcc actggtgtat aaggcattac tgctgaatgc ggcagacgca
     6661 tggcgggagg tgatagtgtt acagcgcagg ctggaggcac tgtcagcgtc accggagaaa
     6721 gcgttgccgc gctcatggtg gcggcgtctt ttacagagag gtacccggtc atgagtaacc
     6781 gttatgtgat tgaggctctg ctgcgtccgg ctgtggaact gaataccgcc gtggtggcag
     6841 caacggcggc cggtgtctgt gtgacagctc cctgggcggt ggcgctcgcg ccttcggtca
     6901 gctatgtcac agccgccggc tttggcgttc tggcagtggt ccgtttccgg caggggatga
     6961 agatcatccg ttaccgtcgc aatctgcgcc ggctgccccg ttatgtgatg agttcccggc
     7021 aaattccggt cagccgtcag cggctgtttc tgggacgcgg cttccgctgg acacagaagc
     7081 acacccagcg ccttcaggac acgctgcgac cggaggttgc gcactatctt cagccgggca
     7141 gtctgtaccg gacagcgcgc tggcttgaaa tgaaaacgga gcgcagtctt ccctggattg
     7201 ggcagcttct tcgtcgtgac tcgccgctga atcccgtccg tcccctgccg ccggtgggag
     7261 gaaatcctgc cctgcatggt attgagccgg atgagcagga tgtgacgctg gcactgggtg
     7321 aacgtgtggg gcacaccatt gtctatggca ccacccgtgt cgggaaaacc cggctggcgg
     7381 agttgctggt cacacaggat attcgtcggg gagatgtcac cattgtcttt gatcccaaag
     7441 gggatgcgga tctgttgctg cgtgtctggg ctgaagcaca ccgtgccggc aggggcgatg
     7501 agctttatat ctttcatctg ggctggccgg aaatctctgc gcgttataac gccgtggggc
     7561 gttttggtcg tgtgtcggag gtggcttccc gtgtggccgg tcagttatcc ggtgaaggta
     7621 acagtgcggc cttccgtgag tttgcatggc ggtttgtcaa cattgttgcc cgtgcactgg
     7681 tggcactggg ggagcgcccg gattacacgc tgattatgcg ttatgtgaat aatattgccg
     7741 acctttatat ccgttatgct ggtaaggtga tcagcgaaca gatgccggaa ctggaaaacg
     7801 ccattctgaa taacatgaat gtactggggg aagcggatgt tccccggacc atgcagaacc
     7861 agccggatgc tgtgcgtatc tgggccattg aggtggctct cagcagtgaa gccggaaaga
     7921 aactctatga tccgatactg gatggtctgc gaagcgcggt acgttatgat cgtacctatt
     7981 ttgacaaaat tgtggcatcc ctgttgccgt tgctggaaaa actgaccaca ggaaagacgg
     8041 cagaactgct ggcacctgac tacctggata tgagcgatac ccgtcccgtg tttgactggg
     8101 agcaggtgat acgtaaaaag gcggtggtgt acattggtct tgatgcgctg agtgacagtg
     8161 aggtggccag tgccgtgggt aactccatgt ttgcggatct ggtgagtgtc gccgggcata
     8221 tctacaaaca cggaatgaat gccggtcttc cgggagggaa tggtaccaaa aatccggtta
     8281 acctgcattg tgatgagttt aatgaactga tgggcgatga gttcattccg ttaatcaaca
     8341 agggaggcgg tgccggtatg caggtgacgg cttacacgca gacctcttct gatattgagg
     8401 cccgtaccgg tagtgctgcc aaaacggccc aggtgcaggg taactttaat aacatgatta
     8461 tgctgcgggt cagggagaac cggacggccg agttactgac gacccagttg tcccaggtgg
     8521 aaatttatac caaaacgctg gtttccggtc atcaggatac cgcagatgtc acgaccggcc
     8581 aggacttcac atccagtacc caggatcgtg ttggtactgt gaaggtgcct ttgctggagc
     8641 cggcagatat cgtgaccctg ccgaaaggac aggcctttgc tctgctggaa gggggacagt
     8701 tgtggaaaat ccgtatgccc ctgcctgcaa ctgatgcaga tgacgcgctg atgccggaga
     8761 gtattgcaaa aattgcagag gccatgcggc gtaattacca caccggagag ggatggtgga
     8821 atgagactcc ctccgtgaac atgccggaag gagagcagaa tggctgaacc ccgtcgtgag
     8881 gtgaacagag cagcacaacc ctctccgcca cgtcagcacg gactgctctg gaatctgttg
     8941 tggggctggc cgtggatgct ggtcgggatc ctgttttcct ccctgctgct gagcctgctg
     9001 gttgagtata tcgggattgc ttttttctgg ccggagcagg gggcagcaca cagtgaggca
     9061 gtgatgaaga cagaactggg gtggctgtct tcggaattca cccgcagtct gctgctgtcg
     9121 acaccatcgg taacggtgac agaatggata gcaacggcgt atcagtggct gtttgtggaa
     9181 acaggttttc tgtcgtggct gaagcagcaa aatgccggtg ccgtggtggg taacagtgtg
     9241 tcacagtaca gtgaggatgt ggggcgctgg ctgagccatt atctgtatga gtacctgatg
     9301 gccacagtat atgtcacggt ggtcacactg atacgggtga cgatcctggt gttgtctgtt
     9361 ccgctgtttg tgatggtgat actgatcgcg gttgtggagg gactggggcg tcgtgatctg
     9421 cgccggtatg gtgctgcgta tgagtccagc tttgtttatc accatgcaaa aaagtctatt
     9481 aaaccggcga tatatattcc ctgcattctt tacttgtcct ggccgacgtc ggtgtatccg
     9541 aacctgctga tatggccggc ggccatgctg ctgggggggg ccatcaccgt gaccatggca
     9601 tccttcaaga aatatttata atccacagag gccggacacc ctgatgcaaa acttgttttg
     9661 tggcagggtg tcggcagaca agccacgccc ttacggtcag tgcgcgtcag tgtcagagta
     9721 acgacagcgc cggaatgaca acaatgttga cgaaaataat gaacagagtg atggccctga
     9781 tgacgtcata gatccacggg gggcaacgag gttcttcata aatgaaaaaa tacacaacac
     9841 aaacccaggt cagaaggaac agggcccaga acggaagggt agtacggaga aattccatgc
     9901 tgatgtgttt cctctttaaa ttgttaatgc ggtgccatta acgggaaaca gtagtgatgc
     9961 cgtgttttaa taaacaggaa taaatcattc aggctggaat aaatttattt gaacagaatc
    10021 tggaacagcg agatgcagaa actggctatg ctgacagccc ccagaacata gtttattctt
    10081 ctggccagct tctctttttt ctgacaggaa ttataaatgt catgaagtga cgtggctaat
    10141 aactgcactt ctgactcact gacatcgggt aaactgatta tattttgtaa ctgttgtttt
    10201 atatcctcag ggagttctgt gaatggggca tatttctcgt accatgtgat cagttctctg
    10261 acccggggga cgtgatgtga aaagttgtta tcagtcagaa aatcattgaa aacagacagc
    10321 agtgcgcttt tgatatgttc atgactggca gaccagcgtt cggccagtaa acagatatca
    10381 ttattttgta aactgccatt tattatcgct tctttcagtt ttttctcgta ctttctcttt
    10441 tttgatgatc tgtctatatc attgatagtc aggataatga atatcaggcc gatgagatag
    10501 actgtcagga gaatgattat actgatgatg tcgttcatgc cagtagttcc gggttgtttg
    10561 ccttcatcca caccagtgta ttctcccgta aaaattttct gttttgcaaa tagagtttat
    10621 cgctgatcca gtcccctgcc ttacgcatcc tgtacgcact gtttaactct gacggaggtg
    10681 cgtatgtttt cttttccggc atcatcctgc cgtgccggcc gtctggccgg tgccgcgggg
    10741 atgctgtttc ttttgtcctt tgcattacct gcgcaggctg ccgaaaagga tgaactggcc
    10801 actgtaatgc gccagcttga ccacgtgcag gcagcgctgg agcgggccag aacggcctct
    10861 ctgcaggctt cttcacaggg ggaacgctac ttttttgatt atttgcgtgc ccgtgcagat
    10921 ctgcagacca ttcgtgacgg tattgaccat tatctggccc cttcccgggc gcaaccccgg
    10981 tcttccgcag ccgtgaccgg tgactaccgg cgggagcgtc cctgatggcg atgacggccc
    11041 tgcagaatac ggccttccgg gctgcttcag gtgaagtgga tctcagtgtg ctgcatctca
    11101 tcacgctggg atttctgctg gcagttgtgt tcctctgggc agcgtggagt ctgacggatg
    11161 cctgggcagg ctggcgtagc ggtgatgtcc gggatggtgt gttcggctgg tttatgttcc
    11221 gactcgtcct gctgctgatc tgcagtatct ggatgtttgc gggttgattt cagtgtcttg
    11281 tttaagtttc aggaggaaat aatgcataaa aaaatgcgca tctgtcgcca ggcctggtat
    11341 gccatggcgg tgtttctgtg taccgggatc acacctgctc tggctgattt gcctcagata
    11401 gagccgccaa catccggcgg aggtggtgga cttttcggac agatcaaagg ctatctccag
    11461 gatggtgttg tgctgtttgg tcttgtgctc agtgcagttg cttttgtgaa ggtggcggaa
    11521 gcggcgcttt ccaccttctc agaggttcgt gacgggaagg ccggatgggg gcagtttggt
    11581 gccatcgtgg tggttggtgt tgttctgctg gtcgccatta tctggctgct gggcaagtcg
    11641 gcctccatca ttctgtaagg agacatcccc atgcagacca ttcgttttct gcccgatcgt
    11701 ctgaacgtgg agcctgcggt tttccgtggt tttaccacac cggaactggg gctggcggcc
    11761 ctgacaggcg ctgtggcagg ggggatctgg ccgttaccgc tgattcctct acttggctgg
    11821 gtgattatcc cgacagggat gttgctgact cccctgctgc tggtctggtt cggtggcgtc
    11881 tggatcagcc ggctgaagcg gggaaaacct gacaactggc tctggcagca tctggcgaag
    11941 aaaagcagtg catggggtgg tggccggaaa acatttatcc gggaggcgca tggctggtcc
    12001 ctgcgccgta cccgtagcag ggggaaaaaa tgagtcgttt tcgtcatgct ctgcgggatc
    12061 gcgaccagca tatcctgaca ctgcgtattt tcagcggtgt gctggccgga attctgatgc
    12121 tggtctgtat cgggtggatg acggcaccgg acagactgac aattcacaat ccgccggatc
    12181 tgcgttccgg cagtacccgt ccctggtggg aagtcccgcc gtcaacggtt tattcctttg
    12241 ccttttatat ctttcagcaa ctgaatgcct ggccgaagaa cggagaggtg gactacccgg
    12301 cgaaaattca tgcgctctct gcctacctga cgccatcctg tcgggagatt ctggagagtg
    12361 atgcccgccg tcgcagtgat gcgggggagc tgctggagcg tgtgcgcgtg gtgtatgaaa
    12421 ttccggaaca tggctacggg ggcaggtaca acaaaaatcc ggtgacggtg gaagggcggg
    12481 ataactggca ggtacagctg gatctggtgg cagatgagta ttttcatgct gagccggtaa
    12541 aacgggcact tgtccgttat ccgataaagg tggtgcgcat ggagggggat cctgaacgta
    12601 atccgttcgg gctggcgctg gactgctaca gcagtacacc acagcggctg gaagcagcct
    12661 ccccggcagc cgggaagggg gataatcagt gatgaacgga gctgaacgtt gtttataccg
    12721 tagtattgtg atggtcctgt cactgctggt tttaccactg agcgcacggg ctgatgagct
    12781 gatgaaatgg gagcgcattc ccttacccgt taccctgatg gcaggacagg agcgggttat
    12841 ttttgcagat aaaaatgtgc gggtgggctt cccgccagca ctgaacggaa agttgagggt
    12901 acagagtacc ggtggggcag tctatctgaa agcagagagt gtttttccgc agacgcgtct
    12961 gcaactgcag gatgtggaga gcggagaaat tattctgctg gatgtgacag cagcagaaaa
    13021 agggcccaca gagcccgtac ggattgtgta tgagggaagc gtcagtacgg tcagtcaggc
    13081 tggcacagcg gcgtctgcac gctctgcaga acacaaaaag gtggtacccg gcggggatga
    13141 cggctcgcag gccctccgtc agccggtgaa gtatgcagag cctttacccg ttctgttgac
    13201 ccgctatgcg gcacagtcgc tgtacgcacc gttacggacg ctggaagcgg taccgggtat
    13261 ccggccggtt aatacacatc tgcccgggaa aatcaccaca ctctatccgt cagagccact
    13321 gacagtcacg ccactggcag cctgggctgc gggtgggcgt gcagttgttg cactgaaact
    13381 gacgagtacg gtaagccgca aaattatcct tgatccgcgg gcacttcagg gaaaattcat
    13441 tgccgcgact ttccagcacc gctggctggg tgcagcaggt acccctgagg ataccaccac
    13501 gctctatgtg gtgacggcag aacggccgga acaggcgttt atcgcggagc ctgcctcccg
    13561 tcgcattcct tcacgggggg aggtgaagca tgcagattaa atccaatacg atcgtgaagt
    13621 atgtggtgcc ctgtgtggtg gtggcgggga tcctgacggg attaaaaagc tgtggaggag
    13681 ataccgggga gccctcgtct gcggaaagct caggaacgct ggccggactg tcaccggatg
    13741 aactgaaggc cctgggcgtg gaaggagaca cacctgaaga taccctgcgc acactgattg
    13801 ggcgtatgaa tcaggtgagg gagcggcaac tggtactgga caaacagaat gaaaagttgc
    13861 tgaaagaaaa tgaaatgctg cgcaagcggg gcacggatgt ttccggtcag gtcagtgagg
    13921 cagtggcagg attgcagaaa aattatgagc agtcgcagcg ccagttccgg aatgaacaga
    13981 acgcgttaat gacacaggtt cagtcactga cagagcaact gaagcgtcag caggagagtg
    14041 ctgatgccgg cgccggagga caggagagcg atattccagc aggtctgggc cttgacggac
    14101 tggggagcgt gacaggatcg gcagcctcac cgggaacgga tggtctgatg tgggtgacac
    14161 cgaccgatca ggtgcaggat gaagcgtcgt cggtggggga taagagcgga ccgcgttttc
    14221 cagtctcttt tctggatgac aatccactga cccgtcagaa agcggcttat gaacagcagg
    14281 tgaagggata cagcaatgaa cagcagaagg cgagcacccg ggcagtgtat accctgccgg
    14341 agaactcgac gctggcaggc agccgggcga tgacagcgct tctgggacgg gttccggtga
    14401 acggaaccgt gacggatccc tatccgttca agatcctggt gggtaaggat aacctgacgg
    14461 caaacggaat agagctgccg gatgtggaag gtgcagtggt gtccggtacc gcgagtggtg
    14521 actggacgct ctcctgtgtc cgtgggcagg tgaacagcat cacgtttgtt tttgccgacg
    14581 ggacagtgcg tacgctgccg tcatcagacg tgaacggcag cagtaacgca caggcaaaaa
    14641 ataacagcag tacaggaggc attggctgga tttcggatga tgacgggatc ccgtgtatca
    14701 gcggaacccg taaatccaat gcctccacgt atctgccgac aatatttgcc ctgtctgcag
    14761 ccggcgctgc gggtgatgca ctgagtgagg ggcagtacac ctcacagaac aacgtcaatg
    14821 gcatcacatc aacactgacc ggcagtgccg gagaggcggc acttggaaaa gccctgtccg
    14881 gcgggatgaa tgaaaccgcg gaatgggtga agcagcgata cgggcagacg tttgatgcca
    14941 tttatgttcc cccggggcag acgctggcgg tgcatatcac gcgtcaactg gccattgatt
    15001 atgaagatca ggggcgccgg gtacgttacg acttcactct gccgggggaa gtccacggta
    15061 ccggtggtct ggactgatgt aaggagatcc cgtgaaacga cagttatctg taatgaccgt
    15121 catggtggcg gtatttattc tgcctggctg ctcgacatcg aaagaggagc ttctgcctcc
    15181 gggtgaacag agtatgctgg agatctggca ggggaaagga agcggaggac attcgcggca
    15241 catgacagcg gccagggaca ccctgcggcg tccgctgaca atgccggagc agcaggcaga
    15301 tgcgcacgga gccataagct acagccggac acaggaaagt gagatcagtc agcagtttcc
    15361 gcggctgccc aatcctgaca tggtgatgta tgtttttccg catctggctt ccggtacgtc
    15421 accggttccg gggtacagca cagtatttcc tttttacagc cggacgcagt atgccatgcc
    15481 gggtgaacga acggaggcgt tgtgatgcga ttttccctgt ttacagacac aaaaaaacgg
    15541 gtttcttccc cggtgtcgca gcatgacgga gaagcggtat ctccggatat gaaagaaaga
    15601 gtggcccctc aggtacgaaa gccactgtca aggccgggga agatgacccg acaggatgag
    15661 acgaaggttt accatgcctg tccgtcggtg attgattttc tgccatgggt ggaatatctg
    15721 gacgaagagc agtgcctgct gctggatgat ggggtgtcgg ttggtgcagt gtatgaggtg
    15781 agtccggccg ctacggaagg gcggaccgca gagcgtctgg agcaaatccg ggatacggtg
    15841 gaggatgcgc tgcaggacag ttttgatgaa tatgactcac atccctgggt ggtgcagttc
    15901 ttctgtcagg atgaaaacga cgtggacacg tatgttgacc agctcagggg gtacgtgaaa
    15961 cctcatgcag aaggctcctc atttacggag gcctggctga gggagatgga gcgccatctg
    16021 aaggggattg cccgaccgga aggtctgttc agggatacgc tggtcacagg tcagccatgg
    16081 cgtggtcagc agcggcgcac gaggatggtg atttaccggt ggatcgggaa aaacaaccac
    16141 gatccgatgc cgccggtggc aatgctgaac caggtctgtt cgcgggtcgt gggggcgctg
    16201 ggtggcgcag gcgtccgctg tacacggatg aacgggcagc aggtgcatgg ctggctgctg
    16261 aggctgttca atccccggcc ggaatgggtg gacagggata tgctgtaccg tatggcatcc
    16321 cgtgcagaac cgcaggagac accggaggga atgatgccgg tgatgaccga ttttgcagaa
    16381 agcctctggt tcaccccccc ggtatctgat ccggaaaacg gcgtgtggtg gctggacggg
    16441 cttccgcatg ccgctgttgt ggtggagaaa ctgcgcacac caccggagcc gggaaccatc
    16501 acaggggaac aggcccgggg cgaaaagaca gtgaatgccc tgatggacac ctttccggag
    16561 ggaacggtgc tgtgtatgac aattgtggtc cagccacagg acacactgga agagcgcttc
    16621 acgaggctgt cgaaaaatgc ggtcggggag aacagtgaat ccctgcgtgc ccgggaggat
    16681 gtggcgaccg tgaagaaata tctgggaaac cggcacaagc tctaccgtgc cgggatcacg
    16741 tttctgctgc gggcagagga tatggagagt ctgaagcgca gacgggttga actgacgacg
    16801 gtgctgctgg gcgcgggctt acagccagtg cgcccggagt ttgatgtcgg accgctgaac
    16861 agctggctgc gcgcgctgcc catgtgtttt gatcctgata cggataaaaa acagtggtac
    16921 acgcgtctga tgtgggtaca gcatctggca gggctcctgc cggtgacggg aagggaaacc
    16981 ggtacggggc atcccggatt cagtttcttt aaccgtggcg gggatgtgct gacctttgat
    17041 ccgctgaata agctggaccg gacccagaat gcacatctgc tgcttttcgg gcctaccgga
    17101 gccggtaaat cggccacgct gtgtggttcg ttgtcgcaga taatggcagt tcatcgtccc
    17161 cgtctgttta tcgctgaggc cgggaactcg tttggtctgc tggcggatta ttttgagagt
    17221 ctcgggcttt cggttaacaa aatcagtgtc aaaccgggaa cgggggtgtg tcttcctcca
    17281 tttgcggatg ctcatcaact ggtggagcag ggagagacgc tgcagagtgt ggatgagcat
    17341 tcactgcccg atcttgatga agatgagggg gatgaagagg aagagaaacg cgacattctg
    17401 ggggagatgg aaatctctgc ccgcatgatg atcaccggag gggatccgaa agaagaagca
    17461 gcgctcaaac gtgcggacag ggcgatgatc cgtgaggcac tgctgatggc cacgcacacc
    17521 acgtaccggg aggggcgtca gatgctgccg gtggatctgc agagtgcgtt gtgggaaatt
    17581 tcgcgggaca ctcagcgtaa tgatgttcgc cgggctaaag ctgctgagat ggcagagtcg
    17641 ctggggatgt tcacccagcc gggcagtttt gaagcggagc tgtttaaccg ggaaggaaaa
    17701 ctgtggccgg aagcggatgt gaccctgatt gatctggggc atcttgcccg tgaaggttat
    17761 gaagcccaga tggcactgac catggtgtca atgaccaata tgattaacaa cattgccgag
    17821 cgcgatcagt atctgggacg ggatattctg tttgtggtgg atgaggcgca tatcgtgacg
    17881 gtaaacccgc ttttgtcccc gtatatgacc aaagtggtga aaatgtggcg caagctgggg
    17941 gcctggctgt ggctggccac gcagaacctg aaggattatc cggatgtctc ggagaagatg
    18001 ctgaatatgg cagagtggtg ggtgtgcctg accatgccgc cggaggaagt gaacaatatt
    18061 gcccgtttta aatccctgac ggaggagcag aaggcggtac tgctgtcagc cagtaaactg
    18121 tccggctgct atacggaggg ggtggtactg gcgaagcgca cagaagcgtt gttccgggtt
    18181 gtgccaccga gtctgtatct ggctctgggg atgacggaga aagaagagaa ggctgaacgc
    18241 cgcgccctga tgaaggtgca ccagtgcagc gaactggaag cagcgtttca tgtggcacga
    18301 aagctggacg ggctacgtgg tctggctggc agggagtgag actatgctgt gtctgaagta
    18361 tccggaaccg gaagtggtgt ctgctgttca ccccgccgga agtgtgtttg ttctgccgcc
    18421 acagggtgcg ccggggataa gttgcaccac acgggataat cttgaacgtc ttcgcgggca
    18481 tctggcggcg caactggggc gggtggagtg tatccgttgc cagccacaga gggtgggtct
    18541 gaacagcagt gtggcggtga tgctggaagg tcagcagggt cagtatgtgc atattctgct
    18601 tactgtgagt gggcatgaga gctggccgtc ggaggaggag tatattcatc cccgctggta
    18661 tatatcagtg actgatgcgg cggatttgtt ctatctgctg ttgtggctgg gagaggggtg
    18721 atatgaggag cgatatcgat tttccgggac cactgtatcc atcgctgtca gatcaaattg
    18781 ccagtgaacc tgtgctgttt ctggcgggag ctgtgggtgt ggcagtgttt tcatgggggc
    18841 ttgcgaattt ttcatggatg cttgcgaatt tactggcccg gatgctgtca gggttgtact
    18901 cccgtgtcgg gaggtgggta aagaactctg aaaaatagcg ggagagaggt gctgccctga
    18961 tgccttcgtt gtattctgcg acgctgttgg tgcccgtatt ttgttgttga gatgagagcc
    19021 agtatgggaa agcgatataa aataactgcg acaataatca aaccaggcaa tatgccggtt
    19081 cagtggctgc gttaccagga tgaaaaaatg actcaggatg aatgtgaaaa actgtttgcg
    19141 cccgcaaaag agccgggacg gtatattggc gtcagggtgg aggacttccg gtgtgaagtg
    19201 gtgaaggcct gagtatgaaa ataaaaacct ccggcggagt cggagggggc aaaaacagat
    19261 agtcccggag tgtatctgtg taaatggtgt tattggtata ataatggtgg taaagaccac
    19321 gggagaattt attcatttta ttcgtcatga aatcaacggt aatagtgtgt ttctgtgacg
    19381 ggaataaaaa aatacagcac agccgatgac ccggtgctga aaacaggtta aatgtagtgg
    19441 gcacatttcc ggtaatatca ggaacagcat gttatggtta taaaagcata taccgggagt
    19501 gtgccctgac actgtgatgt tctggttgtc ttttgaccat tttccgggga cgaaatataa
    19561 gctgttctgc gttaacactt aataatgaga gattgtatgc tgaaaaaaac aattctggcg
    19621 ttatgtattt ccgcaccatt tctggttgcg ggctgtgatg atgcgacaaa ggagaatgta
    19681 agtaagaagg cggcagatat aaaggaagct gcgcagactc aactcagtga tgtgaaggat
    19741 gccgcggagg aagtgaaaat gcaggctgcc ggaaaggtgg cagaggtttc tgaagcggct
    19801 cagtccgggc tcagcgaggt gaaggatgcc gtggaggaag tgaaaacaca ggcagccgga
    19861 aagatggcag aggtctctga agcggctcag tccgggctca gcgaggtgaa ggatgccgtg
    19921 gaggaagtaa aaacacaggc agccggaaag gtggcagagg tctctgaagc gactcagtcc
    19981 ggtctcagtg atgtgaagga taccgctcct gaaaaggggg ctgaaatatc cgtaacggag
    20041 gagtaaaaga gcgccgtcgg tgcagttttt ttcagcaccc ggtgacacgg aattttatat
    20101 acggggcatg tctgtctctt taataaatca acatatttac cggatatccg atgccctgta
    20161 cctgcgctgt ccttatggct ctcaaacaga caatgctttg tctggagcac aggaactctg
    20221 tcatacctgc cggcacaatg cttctgattg ccgggcatct gcgccctgct tttcaggctc
    20281 ataccggacg tttccttatc gctgacctca gtgaggaggt ggtgaggcgg tatctgcggt
    20341 tctgcacgcc attatccggt atggcagtca gcccgtcgtc tttgctttta cagatccggc
    20401 cacagatgtc cgtgctggcg gaatgtctga tcagacaact tattacagat gaaccatcgc
    20461 cggtgtactt cagggatgcg gtggcttttt ccagtctggc gctgtttgag gaacatccgg
    20521 atttccatgc cctgctcacg gggtgtatcc ggagctgcac tgtccgggtg aagaatatta
    20581 tcagtgctga tttgtcccgt ccatggatgc tgggtgaagt ggccagtctg ttatatataa
    20641 gtgagagttt actgaagaaa aagcttcagg aagagctgac gaccttcagt gagcttcttc
    20701 tgaaagagag aatgcagtat gccgttcagc ttttgtctgc gggagaaaac cgtatttccc
    20761 gggtggcaga gttgtgtggc tatgccagtg cttcttattt tatttctgtg ttcaggcgtt
    20821 attatggtat taccccgaat cagtggcttc ctgtgatgca accggattaa tttgattcag
    20881 atgcagagtg tgcgttattc agaggaagag gcttaatttt tatcatccgg ttttgaattt
    20941 attgctgatt gtccctttgc ctttgcgcac gctgtcagat accagttgtc tgacggagtc
    21001 gcatcatgta ccgactgtta ctgtattcct tcctgatact accggtggct gcatctgccg
    21061 gtacaacaat ttataccgat tcgcaccagc gccccatgaa cccgccagca ggtgttcgtg
    21121 ttgtcctgct tgatgctcct gagcagacgc aggatacatt tttcggggta cttcccgcag
    21181 agcccgcaga agcatctgcc cgtgtcatgg aacggatgca gtcgcctgaa tggcagtcgt
    21241 ttcaggcagg gcttgctgaa cactaccgtg cattagcaca tgcctggtcc cttggactga
    21301 aaaaatatcc ggctgtggtt tttgatgaca gtgaagtggt ctacggaacc acagacgtgg
    21361 tgctggcaga acaactgcgt acagggggag ggcagccatg atttcctgca catcacgtca
    21421 tcgcgccggt gttctgctgt taagtatggc ggtattgccg gtggcagagg catcgctgaa
    21481 caccgcgcag atagtggcca gtgcagcctc acagaactgt atcagctggc gggtcagcgg
    21541 gatttgctac tggctgttct gcaccaaatt cagttgcaag gtcagaacat cagtgaaagt
    21601 ttcccattat atcccggaag tggttgtttc cacctatacc gcacctggcg gaaatccctg
    21661 gcgtgaaatg tcggcggtca gcagtacagc cggtggtccg gagaatgcca taagcaaagg
    21721 tattgccggt ctgtcagcag gaggagggaa ccccgcagac atgaaggtac agggggaacg
    21781 tcgttcgtca atccgtttta aatatgccga tgcaacaggg cacccggcca ccacgcttat
    21841 cggagggatg gtgccgggat attcctgtga gtcaccggtg acaccttttg ttccttattt
    21901 tctcagtacg cttgacagta ttgcctggcg tacaggacta ccggagtcac tgtacccgga
    21961 ggccctgatc cccggtaaac gcgaaatagg cagccaggaa gaagagaata tgtggggcaa
    22021 tgtgtatccc cgctccgggt tcgtgaccca gacagatgat gataaagctg ctgcggtggt
    22081 ggcccagcgg gtggctgaca ttatcacccg caccggtgag ccacatgtct atcagccggt
    22141 gaaagcaggg aagaaggacg gttactggcc accggatccg gtggaggaaa atacgggcac
    22201 ccggaaccat aaatggcaga gactgacgcc ttcagtctcc acgtcctgcg cagtcttccc
    22261 tgacggagag catgaagcgg ctgaaacagg taatgctgcc tttgccctgt ggcagcccta
    22321 cagttgctgc aagaaacggg gacagacatt cctgggcagt acgaattttt aagtaagggg
    22381 aagtgatgaa acagaaaatg acgatacctg ttctggctgt ggtgctttgt gcgggtacgg
    22441 tacaggctga cggaaacttt ttgtccctgc cgaaagtgaa tgacagtgcg tttggctacg
    22501 gtaaatccgt ggacggcgca gtatcagaca aactgtttta taccctggga gggggctcgg
    22561 tgatatcgca gccggcaacg cgcagcaata tgcagaaact tggggtggat ctcgggtgga
    22621 gcagtgatct gatgtgtggc aattttgacc tgaaaacaac agtgggtaac cagctgaacg
    22681 ggatcactga tggtttcaaa aacctgatgg gggatgtgat tcagggggcg agcggagccg
    22741 tggcgagtct gccggcaatg gtgatacagc gggctaatcc ggggctgtat gacatgctga
    22801 ccaacggtgt actgcaggcg aatgtggcat tcgacaaagc gcagctcaat tgtcagaaca
    22861 tggcgaaacg catgatggag ttcacggaca gcaataagtg gacgcaggcg gccatgctcg
    22921 aggagtacaa aaaaattgtg aatacggggg acggtgatgc gatcaaaggg gatgaagcag
    22981 gtcgtcaggt taccggggag gaggggcagc gctggatcgg agggcagcag cgtggtggag
    23041 cgggacagtc tgctatccgg ccgacacatg atctgacagc tgccggtttc aatatgatga
    23101 acagtctgcc ttcgaccagt acatccagtg tcagtcccgg gaactgtaac gggagtgcct
    23161 gcatgaaata ccgtaatgca gaggaagctg cccgggcggt ggtgaaggtt ctgggggata
    23221 aatccatgcg gacctgtggt gatgcaaagg catgtaccag cggggatgct gatcaacagc
    23281 cgggaaccag tattgccggc acggggtttg cgccgatgct tgaagaagcg acgcgggtta
    23341 acacggaact gctggcaggt atggtcaatg gtcgtgacaa gccgacagcc gggaatctgg
    23401 caaaactgaa agccgggagt ctgtctgtga cagcgggagt gatccgtgcc ctgcagcgtg
    23461 atccggacaa tggtgccctg acgtcgcgtc tggcgggaga actggccatg gcggataccg
    23521 ttgaaacggc gctgctgatg cgcaggatgc tgctcacggg gatggccgag ccgtatgctg
    23581 cagctcagcc tgctgcactg gaagaggggg aacgccgtat tgaggcgctg gatcgggaaa
    23641 ttatggcgct gaaaaacgag atggagctga agcgggatct ggcgcgtaac agtgttctga
    23701 ccatcattga acgggataac gagcgtgtgg ggaataaccc gatgatccag caggctgaca
    23761 atgctgacag ccgtatccat tctctcgatg ccccggataa ggagtgagag gatgagcaga
    23821 atttatggcc ggatgaaaaa gaggatgaag gtattcgccc tgtatacgct gttgctgaca
    23881 gggattaccg tgttttccct ctggctggcc gataccggta tacgtcacgc agaaggattt
    23941 gcggccctgc gggagtggat gtatgagaca cgttatggct ggctgggatg gcgtctgtgt
    24001 ctttacagtg cgatcggatg gctggggtgg aggatctggc atgcgccggg gttcagggca
    24061 gaataccggg cttcttttct gcgtatggcc acagtcagtg ttctgtttat cctgctgtgt
    24121 gaatacgcca tttttgctga gccggggcgt tgagcaatca tgaccacaaa taactatctg
    24181 gaatattttc tgactctgct gggctgggtg gtgaataacg gtctgtggaa tgtgttgatc
    24241 agcacaggac tgtttgccct gccactggca ttcaggatca tcgggatctg gctgaaagtc
    24301 agggaagaag gtgcggatga gggaaacaaa gggatgcttt ccctgccgcg tattgagcat
    24361 gttctctatg gtgctttttt tgtgatgata tcctgctgtg ttcctctggt acaggtgacg
    24421 ctggatacgc tgtcattcga taaatccagg gcacagacct gtggggtatg gacacctgaa
    24481 gagcccggag agacggggta cagtggggtg atcagcagta taggggatca gactgctgca
    24541 gccccggtat ggtgggtggt ggttcataaa ttgtcgaaag gtgtcaccca ggcggcagtg
    24601 gcttcgattc cgtgccggcc tgatttgcgc cagttgcgtt ttgagatcca gcgaacgttt
    24661 atagaaaaca gggcacttgc tgatgagctg caggacttta cgaacgactg ttattcactg
    24721 gcactgtata tgtggaaaca gcgtgatcag gggcagagta aagataaaaa agtgcttgat
    24781 gatatctcct ggatagggag cgctacattc atgcaggggg attacgggac gctgcagtca
    24841 aagatgccgc gggctgcgtt cccgtggaat acagcacgtg acagcggacg accggatacg
    24901 gggcggggag gctatccgac atgccgggag tggtggagtg acagtagtgt ggggctgaaa
    24961 aaacgggtca tggagggggt cagtgatcat tactggttac gtctgtcagc tgcaatgaag
    25021 atgattggca gtagtcaggt ggattataag gaggaggtga tcaagcgtct ggtgagtccg
    25081 gcaaacctga cgctctctca ggggggagag gtgtatcgtg gctatggtgg caatgcagat
    25141 atgacgacca tgaatgcggt ggcccggatt gcgggaacgg cgggcagtgc ggtagcagca
    25201 ctgggtgcgt ttccggcact ggatgcaatg cggcaggcac tgccgatggt acagtcgata
    25261 atactgatgg cgatttacat catgataccg ctgattctgg cgtttgcggc atatgaactg
    25321 tcgacgttaa tcacactgac gtttgtggtg tttgcggtac actttctgac gttctggtgg
    25381 gagcttgcac ggtggcttga cagctggatg ctgacggcgc tgtacagttc ggatacacat
    25441 acccggttca atatgatggg attccagaac acatcggacg atctgataat gaatctggtg
    25501 atggggacga tgtttctcgt cttgccggca gtgtggctgg gagcgttgtc ctgggctggg
    25561 gtacatgcag gggtaattgc ttctggaatt caacgtggta cagagactgt ttcgggagca
    25621 gcgcagacag caggaactat tgcgagaagt ggtgtgaaat caaaataaca acgtaaatgg
    25681 cggttttacc gccatttact attattggtt tcggcgacaa aaacttagaa tcaataataa
    25741 ttattgatgt tattgatgtt attgatgtta ttgatgtctg ggcatgaagg gaaggttgag
    25801 tctgatgaaa agtttaagtg ttcctcagag tttgttgaat ttacaaatgc gtctaacgag
    25861 ggctgagtat cgggtaatac tgaacaaatg accttttttg caaaggaact gaatgtccaa
    25921 ggattatcca ctggcttaca acgccacatt gctttttcag attcgttatt catatctata
    25981 ccctcggcgc aggataaata acacagtgtt atcatacgtc ttgtatgata aatgtgcaat
    26041 cacatgtctt ccatttgggg tgaagtaaat cggtcggaaa cgttagccct gttagaacgg
    26101 gacgaaacaa tgctgatgtc atgttaatgt cagcattgtt agtaaatcca caacaatttc
    26161 agtcagatta cgcagttggg cgctcgttcc acgagtctgt atactggatg tttccgtcaa
    26221 ctattcgcca ggtgattttt tcatactgca aactgacgct ctcaacgtgg ttcattttat
    26281 cattgcccgc taactttacg ttgggaacgc cgcaattaac gccagttact tttacatttt
    26341 ccatgagcac cgtgtaatag caaacttcct gccctgcatc atttatgtga tagaagcgta
    26401 tttccgcgct tttgagagtc tggcctgtgg ctgcggcctt gtagaggtat ggtgttgaac
    26461 tatcaacttc tttttctatc atcatggctg aatgctggcg agttcctgta attttgccat
    26521 tggcattatc cacagggaga ttgatgccat gactcattcc aatgatctct atgcttcctt
    26581 cacgatcctg gacatcgact gatcccttaa tgtctgcgcc gccgtcgtct tttaaccaca
    26641 tatatggagg gattggcatt ttacttattc ctttttttgc taattatttt catgattacc
    26701 atgtatatag ttgtagtaat tacaaccaca acagaaatat cgatgtaaaa ataagcgtca
    26761 taaactgact ctgcatttat atcgccactg atgaattgcg ctaacttgtc gaccaaatca
    26821 tggtttacat aatcctctgg tttgctgagt gagcgagcaa cgaaaaacat gataatgaaa
    26881 aaaaacacag tctttgctat tttacgggca attgtttgaa ccattagcga ttacctcgat
    26941 ccaaccacga gccataagcg aacgcatgca ggggacttgc accggagttg ttcttaatag
    27001 tgcgtctctt atcaatgcgt aatctgagtt atgagggatg gttatacacc cttctgatat
    27061 tgttcctggg tgtagtctga ataatccacg gtaaactccg tttatccagg tgccatcatc
    27121 aattccccag tcatctttgt ataaggcaaa ccactcattg cgaccaaact ctgcgccata
    27181 gcggattttg ttccatgtgt cttggatttc agcttttcgt tttgaaaaaa aaccaccaga
    27241 gccgcgttca acaatccagt atttccctgg tggtagtggg ccatcacctt taatagcgcc
    27301 acaggctcct ttgtttcggt aaatgccatt accagagaaa gccataaaca cccctacccc
    27361 gtacaaattg aaaggggcgt aatcagcgcc gttcaggata aatttgcctt gtagtgccat
    27421 ttagattgtc ctgaatccat ttccaaattg tgggtatact aacaaaaaac cataagatag
    27481 ataatactcc catggtgagt acacctgaaa acgggagtgg agcgatcttc gtcagtcctg
    27541 tgatttctcg atgctatcat ctgactctgg tgcaggattt ggcaagagtg tattcactgg
    27601 tttacagctc cacattgctt tttcatcttc ctggtgatta aaaatataaa tttttagcag
    27661 aacagaatga ataaaatctt catctggtgt ttttgttgcg cgtaaatgca ggtgtaactg
    27721 tggatgttat ccactgaaga gctgctgatg aggatgaaga gatgctgact aaaaaaatgt
    27781 tactcgtttg tgcttcgtgt gttgcgctga gtgcctgttc tgcacaggaa attgcagagc
    27841 tgaacaggca gataagtgac ggtgcatttg ctgtgacaaa agtactgaac gta
//
LOCUS       DAZKGF010000050        27776 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZKGF010000050 DAZKGF010000000
VERSION     DAZKGF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.050"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..250
                     /locus_tag="ACHOQV_002052"
     CDS             <1..250
                     /locus_tag="ACHOQV_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9630428.1"
                     /translation="LADLERMLHLFSGKPIPDNRENITIHLDDHIRSLQGKECYEDEM
                     FIIKYFKKGSAHITFRKPELVDRLNDIIAKHYPDMLAV"
     gene            complement(400..487)
                     /locus_tag="ACHOQV_002053"
     tRNA            complement(400..487)
                     /locus_tag="ACHOQV_002053"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Ser,seq:gga)
     gene            complement(741..959)
                     /gene="infA"
                     /locus_tag="ACHOQV_002054"
     CDS             complement(741..959)
                     /gene="infA"
                     /locus_tag="ACHOQV_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP9630429.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(1244..1948)
                     /gene="aat"
                     /locus_tag="ACHOQV_002055"
     CDS             complement(1244..1948)
                     /gene="aat"
                     /locus_tag="ACHOQV_002055"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFP9630430.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1990..3711)
                     /gene="cydC"
                     /locus_tag="ACHOQV_002056"
     CDS             complement(1990..3711)
                     /gene="cydC"
                     /locus_tag="ACHOQV_002056"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFP9630431.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFNYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNGSPIASLNEATLRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHNAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3712..5478)
                     /gene="cydD"
                     /locus_tag="ACHOQV_002057"
     CDS             complement(3712..5478)
                     /gene="cydD"
                     /locus_tag="ACHOQV_002057"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFP9630432.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5601..6566)
                     /gene="trxB"
                     /locus_tag="ACHOQV_002058"
     CDS             complement(5601..6566)
                     /gene="trxB"
                     /locus_tag="ACHOQV_002058"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP9630433.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            7110..7604
                     /gene="lrp"
                     /locus_tag="ACHOQV_002059"
     CDS             7110..7604
                     /gene="lrp"
                     /locus_tag="ACHOQV_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFP9630434.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7739..11806
                     /gene="ftsK"
                     /locus_tag="ACHOQV_002060"
     CDS             7739..11806
                     /gene="ftsK"
                     /locus_tag="ACHOQV_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9630435.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAAEQSAQQPYYAPA
                     PEQTAAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQHPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSAGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPIAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPHDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAHHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11965..12576
                     /gene="lolA"
                     /locus_tag="ACHOQV_002061"
     CDS             11965..12576
                     /gene="lolA"
                     /locus_tag="ACHOQV_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFP9630436.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSNFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12587..13930
                     /gene="rarA"
                     /locus_tag="ACHOQV_002062"
                     /gene_synonym="mgsA"
     CDS             12587..13930
                     /gene="rarA"
                     /locus_tag="ACHOQV_002062"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFP9630437.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            14021..15313
                     /gene="serS"
                     /locus_tag="ACHOQV_002063"
     CDS             14021..15313
                     /gene="serS"
                     /locus_tag="ACHOQV_002063"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP9630438.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15552..17996
                     /gene="dmsA"
                     /locus_tag="ACHOQV_002064"
     CDS             15552..17996
                     /gene="dmsA"
                     /locus_tag="ACHOQV_002064"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFP9630439.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSALPAKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            18007..18624
                     /gene="dmsB"
                     /locus_tag="ACHOQV_002065"
     CDS             18007..18624
                     /gene="dmsB"
                     /locus_tag="ACHOQV_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFP9630440.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18626..19489
                     /gene="dmsC"
                     /locus_tag="ACHOQV_002066"
     CDS             18626..19489
                     /gene="dmsC"
                     /locus_tag="ACHOQV_002066"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFP9630441.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19525..20151)
                     /gene="ycaC"
                     /locus_tag="ACHOQV_002067"
     CDS             complement(19525..20151)
                     /gene="ycaC"
                     /locus_tag="ACHOQV_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFP9630442.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            20466..21614
                     /gene="ycaD"
                     /locus_tag="ACHOQV_002068"
     CDS             20466..21614
                     /gene="ycaD"
                     /locus_tag="ACHOQV_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9630443.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            21824..23254
                     /gene="ycaM"
                     /locus_tag="ACHOQV_002069"
     CDS             21824..23254
                     /gene="ycaM"
                     /locus_tag="ACHOQV_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9630444.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(23255..23365)
                     /locus_tag="ACHOQV_002070"
                     /pseudo
     CDS             complement(23255..23365)
                     /locus_tag="ACHOQV_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(23464..24204)
                     /gene="pflA"
                     /locus_tag="ACHOQV_002071"
     CDS             complement(23464..24204)
                     /gene="pflA"
                     /locus_tag="ACHOQV_002071"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFP9630445.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMREVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(24396..26678)
                     /gene="pflB"
                     /locus_tag="ACHOQV_002072"
     CDS             complement(24396..26678)
                     /gene="pflB"
                     /locus_tag="ACHOQV_002072"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9630446.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(26733..27590)
                     /gene="focA"
                     /locus_tag="ACHOQV_002073"
     CDS             complement(26733..27590)
                     /gene="focA"
                     /locus_tag="ACHOQV_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFP9630447.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         6863 a         6839 c         7284 g         6790 t
ORIGIN      
        1 acttgccgac ctggaaagaa tgctgcatct gttcagcggc aaaccgatcc ccgacaacag
       61 ggaaaacata actattcatc tggacgatca catccggtct cttcagggaa aagaatgcta
      121 tgaagatgag atgttcatta ttaaatactt taagaagggc tctgcgcaca tcacgttcag
      181 gaagccagaa ctggttgaca gactgaatga tattatcgca aaacactatc ctgacatgct
      241 ggcagtatag ctttgccagt atgctcaaag atcggaaatt gtccgataag atatgatcgt
      301 ggggcatata aaccggtttc agatggcata cccggtggca tactgtctgg aaggataaat
      361 aaaaaagcgc atgttaaaca ataacatgcg cttgtaatct ggcggtgagg gggggattcg
      421 aacccccgat acgttgccgt atacacactt tccaggcgtg ctccttcagc cactcggaca
      481 cctcaccaaa ttgttttgct accaaacctc atgggtggca acggggcgct actataggga
      541 gttggagtaa aacggtcaag aagaatttta atgataatta ttgtttgctc atactgtaaa
      601 caagttgtgc agtatatcta catcgagaca agttatggac ttatacttcc aaagtacttc
      661 atacatatca caaaataaaa aggccggcta aaccgacctt ttactcgttc tttctcttcg
      721 cccatcaggc ggtaaaacaa tcagcgacta cggaagacaa tgcggccttt gctcaggtcg
      781 tacggggtca gttcaacagt cactttgtcg cccgtcagga tgcggatgta gtttttgcgc
      841 attttaccgg agatgtgtgc agtaaccacg tgaccgtttt ctaactctac gcggaacatg
      901 gtattaggca acgtttcaag aacggtacct tgcatttcaa tattgtcttc tttggccatc
      961 taatcctctg gggtatcact accgtaattt gaaccggcaa gataatgccg aagttctgta
     1021 aataagtaaa gatttgcgcg ctaaatcgca acaaacaggt tcggcacatt actccgaaaa
     1081 cacacggcta agccgcacca aaagcgcaac gtataaggga gcggtgagat aaacgatggg
     1141 cgttacctga cgcgaaaaat tccttatcgg cagcggggta atgagcgtaa ccaactctgc
     1201 gaccgcaatt ataacactct ggggagaaat gtgccgaaaa cattcattct tgtggtgaaa
     1261 acaagcaccg tggtacccag aaattattcg gcaatcgtcc gaggcgcatt tgattgagat
     1321 aattgaggta atcccggcgg ggaatttcgc aggcaccaag cgacgctgtg tgatcgttaa
     1381 ggacctggca gtcgataagt ttaccgccat gaccaataaa ttcctcacag aataccagaa
     1441 gcgccgtttt agacgcattt tccatccggc tgaacatgga ctcgccacaa aatagcgttc
     1501 cctgggccac gccgtacata ccgccgacaa gctcatcttc acgccagact tcaatagagt
     1561 gggcatgacc gagttcgtga aggcgatggt aggcttcgac cacgccacgc gtgatccagg
     1621 ttccttcttc gcgatcgctg gcacagcctt caatgacctg accaaaagcg taattcatcg
     1681 tgactcgata gggcgagcgt ttatgaaatc gcttcatact acggctgata tgcagtgatt
     1741 caggccaaag caccgcgcgg ggatcgggcg accaccagag gatggggtca cctggagaaa
     1801 accacggaaa aataccacgc tggtaagcca ttaacaggcg cgcagggcta agatcgcctc
     1861 ccagtgccag caggccgtta ggctcacgta atgcgccttc cggggaaggg aaggctattg
     1921 aatggcgaga aagctgaacc aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc
     1981 aataatagct tacaaaccct gcttaaactg gtaataacgc ccctgtctgg caagcagttc
     2041 tgcgtgagta ccttgctcaa taatttgccc gttgtccatc actattattt gttggaaacg
     2101 agagagtccg cgaagtcgat gggtgaccat taacaccgtt ttctcatgca tcatttctgc
     2161 aagcaattca aggatctgac tttcggttgt ggcatctaag ccttctgtag gttcatccag
     2221 caataccagc ggcgcattat gtaacagcgc acgggcgata gccagacggc gtagttcacc
     2281 accggagagc tggcgtccgc cttcacccaa ccaactgttg agacctgcat cctcgagcag
     2341 cttctccaga ccaacgcgac gtaagatctc cgccagagcc tcatcactac tgccaggcga
     2401 ggcgagtaac agattatcac gcagcgtggc gctaaacaga tgcactcgct gaggaacaac
     2461 gctgatggtc tgtcgtagag tcgcttcatt caggctggct atggggctac cgttaagcaa
     2521 aatctcgccc tgttgcgggt cccatgcgcg ggtcagcagt tgtaacagtg ttgatttgcc
     2581 gcatccggtt cgcccgagaa tcgctatatg ttccccggcg tttacctgaa gagaaatccc
     2641 tttaagtgcc tgttgggatt gctccggata attgaattga acatcccgta acgtcagcga
     2701 aacgcgatcg gcaacacgag tttgggtatc agggaaggtg acttccggtt tttgatccgt
     2761 taagtcagtg atccgtacgg cagaggcaat gacttgcccc agatgctgaa atgcacccgt
     2821 tactggtgcc agtgcttcaa acgcggctaa cgcgcagaag acaaacaggg caattaacgc
     2881 gccgggttga gcattgccgc caacgccgcc agacgccatc cacagcatca ggatcaccgc
     2941 taacgcgcca atgagcagca ttatcgcttg cgacaatgcg gtgagttcag attgacgacg
     3001 ttgcgcttcc agccactgaa tttctgtatt ctctaattgc gtgcgataac gatcgctggc
     3061 accaaagatg gtcagctcag cttgcccttg cagccaggcc gtcagttgtt ggcgatactg
     3121 cccgcgaaga tgagtcagat tttgcccggt gctttttccc gcacgataaa acagtggtgg
     3181 cataaggaaa agcgtcagta acataatgcc gcccagcgta aaggcgaggg tgaaatcaag
     3241 gaaacttaac ccgattgtca ccaccataat caccacaaaa gcgcccacca gcggcgagat
     3301 aacgcgcagg taaagatgat cgagcgtatc aacatccgcc accacgcggt tgagcaattc
     3361 gccctgacga tagcgcgcca gtccggcagg ggataggggc agcaatttgc tgaaggtgta
     3421 aatgcgcaga tgctgcaaca cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata
     3481 gcgcccggca gtacgggtga ttgctgcgcc acgcacgccc gcagcgggta gcatatagtt
     3541 gaagctgtac agtccggcaa cccccgctac cgctgaggcg gagaggaacc agccggaaag
     3601 tgtcaacaga ccgatactgg cgagcagcgt gacaattgcc agcacaatac caagacttaa
     3661 catccattta tgacgtttat acagtgccag atagggtagc aaagcgcgca tttaaatctc
     3721 ctcctgacga tgggccagca atgtggcgaa aggaccacca gccacgctta attccgcgta
     3781 acgtccttgc tcaataatct gaccatcctg cattacccaa atgacatccc agtcagcaag
     3841 atcttctaac tggtgggtga ccattaacgt tgtctggcgc agagaggcgg cattaagcgc
     3901 ctccattacg cgctgttcac tgtgagcatc aaggctggca gcgggttcat ccaacagtaa
     3961 tagcgaacag ggatttagta atgctcgggc caccgccacg cgctgcgcct gccccacgga
     4021 aaggcgggca gcctggtcgc caacaggcgt atcaacgcct tgtggcagga gcggtagaaa
     4081 ctcgctgacc caggcgttat ccagcgctgt ttgtaactcg tgttcgctgg catcaggtcg
     4141 tgccagtagt acgttatccc gtagtgttgc tgccggtaat tgtgggtttt gcccaaccca
     4201 ggagagatgc ttacgccatg attccggtga taaatcgcgt aattctatcc cgttgattcg
     4261 tagcgatccc tgatatgaga gaaaaccaga aagcgcgttc agcagtgagc ttttacctga
     4321 accgctgcga ccaaccaaca ctgcacgttg gcctgctggc aaagtaaagt tcagcggtcc
     4381 agccagcgtt ttaccttctg gtgatgtaat aaacagatcc tcggcttcaa tggtcaccgg
     4441 atcggttgat gctaattccg cctcaccacg ttgcggatgg gctagcgggg tttccataaa
     4501 cgttttcaga ctgtcagctg cgccaacagc ctgggcttta gcatgataaa acgtaccgag
     4561 atcgcgtaat ggctggaaaa actccggcgc aaggatcagg gccagaaaac ccgcagccag
     4621 cgtcacgcca gtatcatagt gaccaaaatc cagctcgccg agataggaaa aaccaaagta
     4681 gaccgccacc agagcaattg acagcgaggt aaaaaattcg agaatgccgg aggataaaaa
     4741 cgccagccgt agcacttcca ttgtccgttg gcggaaatct tccgaagcag aacgaatact
     4801 ttcaatttca gcttcaccac gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc
     4861 gaggaaatgc ccacttaagc gagcaagagc gagaaagtta cgtcggttag catcggcagc
     4921 ccccattcca accagcgcca taaacaacgg aattaacggt gcagtgccca gcagaataag
     4981 cgccgcagcc cagttagacg ggaagatcgc caccacaatc agcaaaggca cggacactgc
     5041 cagcgccatt tgcggcaggt agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag
     5101 taccagcgtc gcccagctcc ccgccggttt accctgaatc cacgctggcc ctgcttgttg
     5161 cagacggtcg agaacctgac ggcggatggc aaagcggata tgctgcccgg cgtgataacc
     5221 cacccgttcg cgtaaccaga ccacccatgc gcgcagtaca aaggtcagaa ccagtaacgt
     5281 aaagggaagc agcagggctt cacggggaat attctccata atcatatgtt gcagaattcg
     5341 cgccatgaac caggcctggg caatgatcaa tatgccgctc acaaagccca gcagacgaga
     5401 aatattcagc caacgttggg agatgacgct ttgctgtttt aaccagcggg ttaactcttt
     5461 ttgacgggat ttattcattg cacgcttagc aggtgagtta tcagaattat ttgcagagca
     5521 atgttacaac gaggaaaaaa taaaggcgac ccatagtcgc atggtgtcgc cttctttact
     5581 tttgttactg atttgtaaaa ttattttgcg tcagctaaac catcgaggta gcgttccgca
     5641 tcaagtgctg ccatgcagcc tgtaccagca gatgtaatag cctggcgata aatgtgatcc
     5701 atcacgtcgc ctgcggcaaa gacgccaggg atgctggtct gggtggcatt accatgaata
     5761 cccgactgta ctttgatgta gccgttttcc agttccagct gcccttcgaa aatagccgta
     5821 ttcgggctgt gaccgatagc aacaaacaga ccggcaacgt cgagtgactc gatgttatcg
     5881 ctgttttgcg tatcgcgcag acgaacgcca gtgacgccca tctgatcgcc ggtcacctct
     5941 tccagcgtac ggttggtgtg tagaatgatg ttgccgttct ccactttatc catcagacgc
     6001 ttaataagga ttttttccgc gcggaaaccg tcacggcggt gaatcagatg cacttccgaa
     6061 gcgatgttag acagatacag cgcctcttca accgcggtat tgccgccgcc gatgaccgca
     6121 actttctggt tgcgatagaa gaaaccgtcg caggtagcac aagcagaaac cccacggcct
     6181 ttaaaggctt cttcagatgg caggcctaga tagcgtgcag aagctccggt ggcaataatc
     6241 agcgcgtcgc aagtgtattc gccgctatcg ccagtcagac ggaacggacg attctgcaaa
     6301 tcaactttgt tgatgtgatc aaagatgatt tcagtttcaa atttggtggc atgttcgtgc
     6361 atgcgctcca ttaataacgg accggtcaga tcgtttggat cgccaggcca gttttccact
     6421 tccgtggtgg tggtcagttg gccgcctttt tccatgccgg taatcagcac aggttgcagg
     6481 ttggcgcgcg ccgcgtagac agcagcggta tatcccgccg ggcctgaacc caggataagc
     6541 agtttactgt gtttggtcgt gcccatgaga tccccatagt tgttggcaga caatgggcag
     6601 gattgtaggg aatttacaga cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc
     6661 aatcggcagc atcgataagc aggtcaaatt ctcccgtcat tatcacctct gctacttaaa
     6721 tttcccgctt tataagccga ttaaatgatg aataaacgcc cctgttaatg aatatctggc
     6781 atgttgtact aaaaatcgat gttttgcttt gacaatcccc tggtgttttg cgaaaacatt
     6841 cgaggaagaa aaaaacagta ttcttatatg cgcataacca tgcatgtaaa taccatgttt
     6901 accgtgctag tgaaatctac gtatggcgtg gacagacgcc attcgtgatg tcgatagctg
     6961 ccacaaggca acggtcttct caccgtagac ccaggcattg cgcgccgtga atcttcatga
     7021 tttcggtcta tcgtgacggg tagcgactct gaacagtgat gtttcagggt cagacaggag
     7081 tagggaagga atacagagag acaataataa tggtagatag caagaagcgc cctggcaaag
     7141 atctcgaccg tatcgatcgt aacattctta atgagttgca aaaggatggg cgtatttcta
     7201 acgtcgagct ttctaaacgt gtgggacttt ccccaacgcc gtgccttgag cgtgtgcgtc
     7261 ggctggaaag acaagggttt attcagggct atacggcgct gctgaacccc cattatctgg
     7321 atgcatcact tctggtattc gttgagatta ctctgaatcg tggcgcaccg gatgtgtttg
     7381 aacaattcaa taccgctgta caaaaacttg aagaaattca ggagtgtcat ttagtatccg
     7441 gtgatttcga ctacctgttg aaaacacgcg tgccggatat gtcagcttac cgtaagttac
     7501 tgggggaaac cctgctgcgt ctgcctggcg tcaatgacac acggacatac gttgttatgg
     7561 aagaagtcaa gcagagtaat cgtctggtta ttaagacgcg ctaacacgga acaggtgcaa
     7621 aatcggcgta ttttgattac actcctgtta atccatacag caacagtact ggggtaacct
     7681 ggtactgttg tccgttttag catcgggcag gaaaagcctg taacctggag agcctttctt
     7741 gagccaggaa tacactgaag acaaagaagt cacattgaca aagttaagta gcggccgccg
     7801 ccttctggag gcgttgctga tccttattgt cctgtttgcc gtctggttga tggctgcctt
     7861 actaagcttt aacccttcgg accccagctg gtcgcaaacg gcctggcatg aacctatcca
     7921 taatttaggt gggatgcccg gtgcgtggct ggcagatacg ctgttcttta tttttggcgt
     7981 gatggcttac accattcccg tcattattgt cggcggttgt tggtttgcct ggcgtcatca
     8041 gtccagcgac gaatacattg attattttgc cgtttcgcta cgcatcattg gcgttttggc
     8101 gctcatcctt acctcctgtg gtctggcggc aatcaacgct gacgatatct ggtattttgc
     8161 ctccggtggc gtcattggca gcttactaag cactacgcta caaccactgc tacacagtag
     8221 cgggggaact attgcgctgc tctgcgtttg ggctgcgggt ctgacgctgt tcaccggttg
     8281 gtcatgggtg accattgctg aaaaactcgg cggctggatt ttaaacattc tcaccttcgc
     8341 cagtaatcgt acccgtcgcg atgatacctg ggtcgatgaa gatgagtatg aagacgacga
     8401 agaatatgaa gatgaaaatc acggtaaaca gcatgaatca cgccgtgccc gtattcttcg
     8461 cggcgcgtta gcgcgtcgta aacggttggc ggaaaaattc attaatccga tggggcggca
     8521 aacagatgct gcgttgttct ccggtaagcg gatggatgat gaagaagaga ttacctacac
     8581 tgcacgcggt gtggctgccg accctgacga cgtcctcttt tcgggcaatc gtgcaacgca
     8641 gccagaatat gacgaatacg atccattatt aaacggtgcg ccaattaccg aacctgttgc
     8701 tgtagcagct gctgctacca cggcgacaca aagctgggct gcgccggttg aacctgtgac
     8761 tcagacgccg cctgttgcct ctgttgatgt tccaccttcg caacctacag tagcctggca
     8821 gcctgtaccg ggtccacaaa cgggagagcc ggtgattgct ccggcaccgg aaggttaccc
     8881 acagcagcca caatatgcgc agcctgcagt gcaatataat gagccgctgc aacaacctgt
     8941 acagccacag cagccgtatt atgcgccagc agcggaacaa cctgtgcaac aaccttacta
     9001 cgctcctgcg gcggaacaac ctgttcagca gccgtattat gcaactgcgg cggaacagtc
     9061 tgcgcaacag ccgtattacg cccctgcgcc agaacaaacg gcggcaggta acgcctggca
     9121 agccgaagag cagcaatcca cttttgctcc acagtctaca taccagactg agcaaactta
     9181 tcagcagcca gccgctcagg agccgttgta ccaacagccg caacccgttg aacagcaccc
     9241 tgttgtggag cctgagcccg ttgtagaaga gacaaaaccc gcgcgtccgc cgctttacta
     9301 ttttgaagaa gtggaagaga agcgtgcccg tgaacgtgaa caacttgcgg cctggtatca
     9361 accgattcct gaaccggtta aagaaccaga accgatcaaa tcttcgctga aagcaccttc
     9421 tgttgcagca gtacctccag tggaagccgc tgccgctgtt tctccgctgg catctggcgt
     9481 gaaaaaagcg acactggcga cgggggctgc cgcaaccgtt gccgcgccag tcttcagtct
     9541 ggcaaatagc gctggaccgc gtccgcaggt caaagagggg attggcccgc agttgccacg
     9601 accgaaacgt atccgcgtgc caactcgtcg tgaactggcg tcttacggta ttaagctgcc
     9661 ctcacagcgt gcggcagaag aaaaagcccg tgaagctcag cgcaatcagt acgattccgg
     9721 cgatcagtat aacgatgatg aaatcgatgc gatgcagcag gatgaactgg cacgtcagtt
     9781 cgcccagaca cagcagcaac gctatggcga acagtatcaa catgatgtgc ccgtgaacgc
     9841 agaagatgca gatgctgcgg cagaggctga actggctcgt cagtttgctc aaactcaaca
     9901 acaacgttat tccggcgaac aacctgctgg ggcgaatccg ttcactctgg atgattttga
     9961 attttcgcca atgaaagcgt tgctggatga tggtccacac gaaccgctgt ttacgccaat
    10021 tgttgaacct gtacagcagc cgcaacaacc gattgcaccg cagcaacagt atcagcagcc
    10081 gcagcaacca gttgcgccgc agccgcagta tcagcagccg caacagccgg ttgcaccgca
    10141 gcagcaatat cagcagccac aacaaccggt tgcaccgcag ccgcaatatc agcagccgca
    10201 acagccggtt gcaccacagc cgcaatatca gcagccgcaa cagccagttg cgccgcagcc
    10261 gcatgatacc ctgcttcatc cgctgttgat gcgcaatggt gacagccgtc cgttgcataa
    10321 accgacgacg ccgctgcctt ctctggattt gctgacgccg ccgccgagcg aagtggagcc
    10381 ggtagatacc tttgcgcttg aacaaatggc gcgcctggtg gaagcgcgtc tggctgattt
    10441 ccgtattaaa gccgatgtcg tcaattattc tccggggccg gttatcactc gctttgaatt
    10501 gaacctggca ccgggagtaa aagcggcgcg tatttctaac ttgtcacgtg accttgcccg
    10561 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    10621 gctggagtta ccgaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    10681 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    10741 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    10801 cggtaagtct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    10861 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    10921 cccgcatctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    10981 gtgtgttaac gagatggagc gtcgttataa actgatgtct gcgctgggtg tgcgtaatct
    11041 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    11101 gtactggaag ccgggtgata gtatggatgc gcatcatccg gtgctgaaaa aagaaccgta
    11161 catagtggtg ttggtggacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    11221 agagctgata gcacgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    11281 aacgcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    11341 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    11401 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    11461 acgtgtccat ggtgcttttg tacgcgatca ggaagttcat gccgtggtgc aggactggaa
    11521 agcgcgtggt cgcccacagt atgttgacgg catcacctcc gacagcgaaa gcgaaggtgg
    11581 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    11641 tgtcactgaa aaacgcaaag cgtcaatttc aggcgtacag cgtcagttcc gcattggtta
    11701 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcaa gggattgtca gcgaacaggg
    11761 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcatcgtag
    11821 gccggataag gcgcggtagc gtcgcatccg gcactctaac aactgaaaat tcagtatttt
    11881 cttctttcct caagctgatt attagcctgg aatagagagt agagggaact cccgatcggg
    11941 agtgacgtaa tttgaggaat aatgatgaaa aaaattgcca tcacctgtgc attactctca
    12001 agcttagtag caagcagcgt ttgggctgat gccgcaagcg atcttaaaag ccgcctggat
    12061 aaagtcagca acttccacgc cagcttcaca caaaaagtga ctgacggtag cggcgcggcg
    12121 gtgcaggaag gccagggcga tctgtgggtg aaacgtccaa atttattcaa ctggcatatg
    12181 actcaacctg atgaaagcat tctggtttct gacggtaaaa cactgtggtt ctataacccg
    12241 ttcgttgagc aagctacggc aacctggctg aaagatgcca ccggtaatac gccgtttatg
    12301 ctgattgccc gcaaccagtc cagcgactgg cagcagtaca atatcaaaca gaatggcgat
    12361 gactttgtcc tgacgccgaa agccagcaat ggcaatctga agcagttcac cattaacgtg
    12421 ggacgtgatg gcacaatcca tcagtttagc gcggtggagc aggacgatca gcgcagcagt
    12481 tatcagctga aatcccagca aaatggggct gtggatgcag cgaaatttac cttcaccccg
    12541 ccgcaaggcg tcacggtaga tgatcaacgt aagtagaggc acctgagtga gcaatctgtc
    12601 gctcgatttt tcggataata cttttcaacc tctggccgcg cgtatgcggc cagaaaattt
    12661 agcacagtat atcggccagc aacatttgct ggctgcgggg aagccgttgc cgcgcgctat
    12721 cgaagccggg catttgcatt ctatgatcct ctgggggcca ccgggtaccg gcaaaacaac
    12781 cctcgctgaa gtgattgccc gctatgcgaa cgctgatgtg gaacgtattt ctgctgtcac
    12841 ctctggcgtg aaagagattc gcgaggcgat tgagcgcgct cggcaaaacc gcaatgcagg
    12901 tcgccgcact attctttttg ttgacgaagt tcaccgtttc aacaaaagcc agcaggatgc
    12961 atttctgcca catattgaag acggcaccat cacttttatt ggcgcaacca ctgaaaaccc
    13021 gtcgtttgaa cttaactcgg cactgctttc ccgtgcccgt gtctatctgc tgaaatccct
    13081 gagtacagag gatattgagc aggtgcttac tcaggcgatg gaagacaaaa cccgtggcta
    13141 tggtggtcag gatattgttc tgccagatga aacacgacgt gccattgctg aactggtgaa
    13201 tggcgacgcg cgccgggcgt taaatacgct ggaaatgatg gcggatatgg ccgaagtcga
    13261 tgatagcggt aagcgggtcc tgaagcctga attactgacc gaaatcgccg gtgaacgtag
    13321 cgcccgcttt gataacaaag gagatcgctt ttacgatctg atttccgcac tgcataagtc
    13381 ggtacgtggt agcgcacccg atgcggcgct gtactggtat gcgcgaatta ttaccgctgg
    13441 tggcgatccg ttatatgtcg cgcgtcgctg tctggcgatt gcgtctgaag acgtcggtaa
    13501 tgccgatcca cgggcgatgc aggtggcaat tgcggcctgg gattgcttta ctcgcgttgg
    13561 cccggcggaa ggtgaacgcg ccattgctca ggcgattgtt tacctggctt gcgcgccaaa
    13621 aagcaacgct gtctacactg cgtttaaagc cgcgctggcg gatgcacgcg aacgtccgga
    13681 ttatgacgtg ccggttcatt tgcgtaatgc gccgacgaaa ttaatgaagg aaatgggcta
    13741 cgggcaggaa tatcgttacg ctcatgatga agcaaacgct tatgctgccg gtgaggttta
    13801 tttcccgccg gaaatagcac aaacacgcta ttatttcccg acaaacaggg gccttgaagg
    13861 caagattggc gaaaagctcg cctggctggc tgaacaggat caaaatagcc ccataaaacg
    13921 ctaccgttaa tgttatcgtt gcggtaatgt tgttactgta tccctgtggt cgcaggctgt
    13981 ggccacatct cccatttaat tcgataagca caggataagc atgctcgatc ccaatctgct
    14041 gcgtaatgag ccagacgcag tcgctgaaaa actggcacgc cggggcttta agctggatgt
    14101 agataagctg ggcgctcttg aagagcgtcg taaagtattg caggtcaaaa cggaaaacct
    14161 gcaagcggag cgtaactccc gatcgaaatc cattggccag gcgaaagcgc gcggggaaga
    14221 tatcgagcct ttacgtctgg aagtgaacaa actgggcgaa gagctggatg cagcaaaagc
    14281 cgagctggat gctttacagg ctgaaattcg cgatatcgcg ctaaccatcc ctaacctgcc
    14341 tgcagatgaa gtgccggtag gtaaagacga aaatgacaac gttgaagtca gccgctgggg
    14401 caccccgcgt gagtttgact ttgaagttcg tgaccatgtg acgctgggtg aaatgcactc
    14461 tggcctcgac tttgcagccg cagttaagtt gaccggttcc cgctttgtgg taatgaaagg
    14521 gcagattgct cgcatgcacc gcgcactgtc gcagtttatg ctggatctgc ataccgaaca
    14581 gcatggctac agtgagaact atgttccgta cctggttaac caggacacgc tgtacggtac
    14641 gggtcagctg ccgaaatttg ctggcgatct gttccatact cgtccgctgg aagaagaagc
    14701 agacaccagt aactatgcgc tgatcccaac ggcagaagtt ccgctgacca acctggtgcg
    14761 cggtgaaatc atcgatgaag atgatctgcc aattaagatg accgcccaca ccccatgctt
    14821 ccgttctgaa gccggctcat atggtcgtga cacccgtggt ctgatccgta tgcaccagtt
    14881 cgacaaagtt gaaatggtgc agatcgtgcg cccggaagac tcaatggcgg cgctggaaga
    14941 gatgactggt catgcagaaa aagtcctgca gttgctgggc ctgccgtacc gtaaaatcat
    15001 cctttgcact ggcgacatgg gctttggcgc ttgcaaaact tacgacctgg aagtatggat
    15061 cccggcacag aacacctacc gtgagatctc ttcctgctcc aacgtttggg atttccaggc
    15121 acgtcgtatg caggcacgtt gccgcagcaa gtcggacaag aaaacccgtc tggttcatac
    15181 cctgaacggt tctggtctgg ctgttggtcg tacgctggtt gcagtaatgg aaaactatca
    15241 gcaggctgat ggtcgtattg aagtaccaga agttctgcgt ccgtatatga acggactgga
    15301 atatattggc taatacccaa tttttctgaa tctaaaaagc gcctgcgggc gctttttttg
    15361 tctccctttg ataccgaaca ataattactc ctcacttaca cgtaatacta ctttcgagtg
    15421 aaaatctacc tatctctttg attttcaaat tattcgatgt atacaagcct atatagcgaa
    15481 ctgctataga aataattaca caatacggtt tgttactgga atcaatcgtg agcaagcttg
    15541 agtgagccat tatgaaaacg aaaatccctg atgcggtatt ggctgctgag gtgagtcgcc
    15601 gtggtttggt aaaaacgaca gcgatcggcg gcctggcaat ggcaagcagc gcgttaacat
    15661 taccttttag tcggattgcg cacgctgttg atagtgccct tccggcaaaa tcagacgaaa
    15721 aggttatctg gagcgcctgt acagttaact gtggtagtcg ctgcccgcta cgtatgcacg
    15781 ttgtggacgg tgaaattaaa tatgttgaaa cggataatac cggcgatgac aactatgacg
    15841 gcctgcacca ggtgcgcgcc tgtctgcgtg gtcgttccat gcgtcgccgt gtctacaatc
    15901 cggaccgcct gaaatatccg atgaaacgag tcggggcgcg cggtgaaggc aaattcgagc
    15961 gcattagctg ggaagaagcc tacgacatca tcgcgaccaa tatgcagcgc ctgatcaaag
    16021 agtacggcaa cgagtctatc tatctgaact atggtaccgg aacgctgggc ggcaccatga
    16081 cccgctcctg gccgccggga aataccctgg tcgctcgcct gatgaactgc tgcggcggtt
    16141 atctgaacca ttacggtgac tactcttccg cgcaaattgc ggaaggtttg aactatacct
    16201 acggcggctg ggcagatggt aacagcccgt cggatatcga aaacagtaaa ctggtagtgc
    16261 tgtttggtaa taaccctggc gaaacgcgaa tgagtggcgg tggggtgact tactatcttg
    16321 aacaggcgcg ccagaaatct aatgctcgca tgatcatcat cgatccgcgc tataccgaca
    16381 ccggtgccgg gcgcgaagat gagtggatcc ctattcgtcc gggaacagat gccgcactgg
    16441 ttaacggtct ggcgtatgtc atgatcaccg aaaacctggt ggatcaggca ttcctcgata
    16501 aatattgcgt tggctacgac gagaaaacac tgccagccag tgcgccgaaa aatggtcact
    16561 ataaagctta tattctgggt gaagggccag atggcgtggc taaaacgccg gaatgggcct
    16621 cgcaaatcac tggtgttccg gcagacaaaa tcatcaaact ggctcgtgaa atcggtagta
    16681 ccaaaccggc gtttatcagc cagggatggg gcccgcagcg tcacgctaac ggtgaaatcg
    16741 caacccgtgc tatctcgatg ctggcgattc tgaccggtaa cgttggtatt aacggaggca
    16801 acagcggcgc gcgtgaaggt tcatacagct taccgtttgt ccgtatgccg accctcgaaa
    16861 acccgatcca gaccagcatt tcgatgttta tgtggaccga tgccattgaa cgtggcccgg
    16921 aaatgacagc gctgcgtgat ggtgtgcgcg ggaaagataa gctggatgtg ccgatcaaaa
    16981 tgatctggaa ctatgccggt aactgcctga ttaatcagca ttctgaaatc aaccgtaccc
    17041 atgaaatcct tcaggatgat aagaagtgcg agctgattgt ggttatcgac tgccacatga
    17101 cctcatcggc gaaatatgct gacatcctgc tgcctgactg caccgcttca gaacagatgg
    17161 actttgcact ggatgcatcc tgcgggaata tgtcttacgt gattttcaac gatcaggtga
    17221 ttaaaccgcg ctttgaatgt aagaccatct atgaaatgac cagcgaactg gcaaaacgtc
    17281 ttggcgttga gcaacagttt actgagggcc gtacccagga agagtggatg cggcatctgt
    17341 atgcccagtc gcgggaagcg attcctgaac tgccaacgtt tgaagagttc cgtaagcagg
    17401 ggatctttaa aaagcgcgac ccacaagggc atcacgttgc ttataaagct ttccgtgaag
    17461 atccgcaggc aaatccactg accacgccat cggggaaaat tgagatttat tcgcaggcgc
    17521 tggctgacat tgccgctacc tgggaattgc cagaaggtga tgtgatcgat ccactgccga
    17581 tctacacgcc gggctttgaa agttatcagg atccgctgaa caaacagtat ccgctgcagc
    17641 ttacgggttt ccactataaa tctcgcgttc actcaactta cggcaacgtt gatgtgctga
    17701 aagcagcttg ccgtcaggaa atgtggatca acccgcttga tgcgcaaaaa cgcggtatca
    17761 acaacggcga taaagtcagg atctttaacg atcgtggtga ggttcatatt gaggcgaaag
    17821 tgacgccacg aatgatgccg ggtgtggtcg cactgggtga aggtgcctgg tatgacccgg
    17881 atacaaaacg tgtcgataag ggcggttgta tcaacgtact gaccactcaa cgtccgtctc
    17941 ctctcgctaa ggggaatccg tcacatacaa acctggttca ggttgaaaag gtgtaaggag
    18001 taaccgatga caacccagta tggatttttt attgattcca gccgttgcac cggttgcaaa
    18061 acctgcgagc tggcttgtaa agactacaaa gatttgacgc cagaagtcag tttccgccgc
    18121 atttatgaat atgctggcgg cgactggcag gaagataacg gtgtctggca ccagaacgtg
    18181 tttgcctact atctgtcgat ttcatgtaac cactgcgaag atccggcttg tactaaagtc
    18241 tgcccgagcg gtgcgatgca taaacgtgaa gatggttttg ttgtggtcga tgaagatgtg
    18301 tgcattggct gccgttactg ccatatggct tgcccgtacg gcgcaccgca atataacgaa
    18361 acgaaaggcc atatgaccaa atgcgatggt tgttatgacc gtgttgcgga ggggaaaaag
    18421 ccgatctgtg ttgaatcttg cccgctgcgg gcgctggatt tcggccctat cgacgagctg
    18481 cgtaaaaaac atggcgatct ggcggccgtt gcgccgttgc cgcgagctca ctttaccaaa
    18541 ccgaatattg tgatcaaacc caatgccaac agccgcccga ccggggatac cactggctat
    18601 ctggcaaacc cgaaggaggt gtgagatggg aagtggatgg catgaatggc cgctgatgat
    18661 cttcacggtc ttcgggcaat gtgtagcggg tggttttatc gttctggctt tggcgctgct
    18721 caaaggcgac ctgcgagcag aagcccagca gcgtgttatt gcctgcatgt ttggtttatg
    18781 ggtgctgatg ggcattggct ttatcgcctc tatgcttcat cttggttcac cgatgcgcgc
    18841 ctttaactcg ctcaaccggg taggggcttc agcactcagt aacgaaatcg ccagcggttc
    18901 gatctttttt gctgttggtg gcatcggctg gttgctggca atgctgaaaa agctgtcacc
    18961 ggcattgcgt acgctgtggc tgatagtgac gatggttctt ggcgtcatct ttgtctggat
    19021 gatggtgcgt gtgtataaca gcattgatac cgttccgacc tggtacagca tctggacgcc
    19081 gatgggcttc ttcctgacga tgttcatggg cggcccgtta ctgggttacc tgttgctgag
    19141 tctggctggc gtcgatggct gggcgatgcg tctgctgcca gccatttctg tactggcact
    19201 ggtagtgagt ggcgtgatgt cagtgatgca gggcgcagag ctggcgacta ttcatagctc
    19261 tgtgcagcag gccgcagcgc tggtgccgga ctatggcgcg ctgatgtcct ggcggatcgt
    19321 gcttttggct gttgccctgt gcttgtggat tgcaccacag ctaaaaggtt atcagcctgc
    19381 ggttccgcta ctttctgtat cattcattct gcttctggca ggggaattaa tcggtcgtgg
    19441 cgtattctat ggtttgcata tgaccgtggg gatggctgtc gcaagttaat cagtaacaac
    19501 cggggtttcg gccccggttc gcttttattt ctgcttcgtt aacgtgtcat aactggtcat
    19561 caagttacga taatcaggaa tgtggttaga gaacaacgtc gccagtcctt caatatcatt
    19621 acgccagtcg cgatgcagct cgcatgccac gccgaaccat gtcatcaatt gcgcaccagc
    19681 ttgcgacatg cgatcccatg ccgaatgccg ggtaatttca ttaaaggtcc ctgacgcgtc
    19741 agtcacgaca aagacgtcaa acccttcttc aatggctgaa agcgccggga atgccacgca
    19801 aacttcggtt accacaccgg caataattaa ctgtttttta cctgtcgctt tgacagcttt
    19861 tacaaaatct tcgttatccc aggcgttaat atttcccggg cgagcaatgt aaggtgcatc
    19921 ggggaattgt gctttcagtt ctggaactaa tgggccgtta ggaccggttt caaaactggt
    19981 ggtaagaata gttggtaagt tgaaatactt agccaggtca cccagcgcca gcacgttatt
    20041 tttaaactta tcgggttcga tatcccgtac aagggaaagt aaaccagcct ggtgatcaac
    20101 aagcaaaacg gcagcatcat ttttatcaag acgaacatac ggtttggtca tcctcttctc
    20161 ctttcgagag tcagtggtac gtctgaaatc ggtcagacgc gatgcattgc tctgaaagca
    20221 tagacgggaa atatgagttt gctgtgacca tgaaattttt cgactgaacg aagcgtatga
    20281 aatttgtgtt agatcaataa aaacaatcag atgagattaa tcgtctctcc agcattagcg
    20341 ttatgaatga gtaatataaa tcgataatac ctccgctgcg atatgcgcgt attttcaccc
    20401 attgacaatg tttttggcgg tggcatgatg cgcatgaaat ttgaacttcc tcacggtttt
    20461 aattcatgtc cacgtatacc cggcctgtca tgcttttgct gtctggcctg cttttgttga
    20521 ctctggcgat cgcagtgtta aatacactcg tgccgctttg gctcgcccag gaacacatgt
    20581 ccacatggca ggtaggcgtt gtcagctcat cctattttac cggcaacctg gtcggtacat
    20641 tgctgacagg gtatgtcatt aagcgcattg gctttaaccg cagctattat ctggcctcct
    20701 tcatttttgc cgctggctgt gccggccttg gcctgatgat tggattctgg agctggttgg
    20761 cttggcgttt tgtcgccggt gtcggttgtg ccatgatttg ggtggttgtt gagagcgcgc
    20821 tgatgtgcag tgggacgtca cgtaaccgtg ggcgtttgct tgctgcgtat atgatggttt
    20881 attacgtggg aacgttttta ggccagttac tggtcagcaa agtttcaacc gagctgatgt
    20941 ccgtattgcc gtgggttaca ggtttgacgt tggcagggat cttaccgctg ttgtttacgc
    21001 gtgtgctgaa tcagcaggct gaaaaccatg attcgacgtc aattacgtca atgctaaaac
    21061 tccgtcaggc gcggcttggc gtgaatggct gcattatctc aggaatcgtg ctgggatctc
    21121 tatatggcct gatgccgctg tacctcaatc acaaaggggt gagcaatgcc agcattggtt
    21181 tctggatggc ggtactggtc agtgcgggta tcctcggaca atggccgatc ggacgtctgg
    21241 cggataagtt tggtcgactg ctggtgttgc gtgttcaggt ctttgtcgtc attctcggca
    21301 gtatcgcgat gcttagccag gcggcgatgg ccccagcgtt attcatcctc ggtgccgctg
    21361 gctttacgct atatccggtg gcgatggcct gggcttgtga gaaagttgaa catcatcaac
    21421 tggtggcgat gaaccaggcc ttactgttga gctataccgt gggaagtctg cttggcccgt
    21481 catttaccgc tatgctaatg cagaatttct ccgataattt attgtttatc atgatcgcca
    21541 gcgtatcgtt tatctatttg ctgatgctgc ttcgcaacgc cggtcatacg ccgaaacccg
    21601 ttgctcacgt gtaaatgaat tcaagcagag tgtgaactta ctgtttcaca ctctgctttt
    21661 ttgtttcttc tatctgactt gctttattcc aaattttatt cgtttaaaaa taaaatgtgc
    21721 agcaggttat aattttgcat ttcgctattt ccgcacttct tatttgccgc gcataatccc
    21781 tcgttttaca gatgcccctt taattttggc gaaggatttg tctatggctg ggaatgttca
    21841 ggaaaaacag ctgcgatggt acaacattgc gctgatgtct tttatcactg tctggggttt
    21901 tggcaatgtt gttaataact acgccaacca ggggctggtg gttgtttttt catgggtgtt
    21961 tatctttgcg ctctatttca caccttatgc gctaattgtt ggtcagttag gctcgacctt
    22021 caaagatggg aagggcgggg tcagtacctg gattaaacac acgatgggac ccggactggc
    22081 ttacctcgcc gcgtggacct actgggtagt gcatattccc tatctggcac aaaaacccca
    22141 ggcaatactg attgcgctcg gttgggcgat gaaaggcgac ggttcgttaa tcaaagaata
    22201 ttcagtcgta gcgttacagg ggttaacgct ggtgctgttt atcttcttta tgtgggttgc
    22261 ttcacgcggt atgaaatcgc tgaaaatcgt cggttctgtg gcagggattg cgatgtttgt
    22321 tatgtcgctc ctgtatgtgg cgatggcggt aaccgcgcct gcaattactg aagtgcatat
    22381 tgcgaccaca aacattacct gggaaacgtt cattcctcat atcgacttta cctacattac
    22441 cactatttca atgctggttt tcgcggttgg cggagcagag aagatttctc cttacgttaa
    22501 tcaaacgcgc aacccaggaa aagaatttcc aaaagggatg ttatgcctgg cggtgatggt
    22561 tgcggtttgt gccattctgg gttcactggc gatggggatg atgtttgact cgcgtaatat
    22621 cccggatgac ttaatgacta acggtcagta ttacgccttt cagaagctgg gcgagtatta
    22681 caacatgggt aatactttaa tggtgattta cgccattgcg aataccctgg gacaagtagc
    22741 tgcgctggta ttctcgattg atgccccgct taaagtgcta ttaggcgatg ctgacagcaa
    22801 atatattcca gccagtttat gtcgtaccaa cgcttctggt acgcccgtta atggctattt
    22861 tctgaccctg gtgctggtgg cgatcctgat aatgctaccg actctcggca ttggtgatat
    22921 gaacaatctc tataaatggc tgttgaatct taactcggta gtgatgccgc tgcgttattt
    22981 atgggtattt gttgcattta ttgcagttgt tcgcctggcg cagaaatata agccggaata
    23041 tgtctttatt cgtaataagc cgctggcaat gaccgtcgga atttggtgtt ttgcctttac
    23101 cgcttttgcc tgcctgactg gtattttccc gaaaatggaa gccttcaccg cagagtggac
    23161 attccagttg gcgctgaatg ttgcaacgcc gtttgtgctg gtaggattag ggctgatatt
    23221 cccgctgctg gcgcgtaaag cgaatagcaa ataattattg tggtcggtga tgctgactct
    23281 ggcagatctg acccgttttt acataatcga gaaatgcgcg caagccgcag gacatatact
    23341 ggcggtttgg gtaatacatt tggaagctgt gctgtaaggg cgttagaaat agagctctga
    23401 attatttatg cagcgcggaa taaaaaagcg gcaactcaaa aaagttgccg ctttacgggg
    23461 aaattagaac attaccttat gaccgtactg ctcaagaatg cctttcacgc gttccatggt
    23521 ctctttcttc ggtggtttaa caccatcaag tttgtattct tcacccatcg ccacccattt
    23581 gtgtttgccc agttcgtggt aggggagaag ctcgattttc tcaacgttgc ccatatcacg
    23641 ggtaaattca ccgaggcgat gcgctgaatc gtcatcgtca gaccagcctg ggacaacaac
    23701 gtagcggatc cacaccttca catttttgtt cgccagatat ttggcgaact ccagcgtgcg
    23761 gtggttggaa actccaacca gattttggtg gatctcgtcg ttcatctgtt tgagatcgag
    23821 cattaccagg tcggttactt ccagcagttc atcaatcacc ggatcgtaac gacgaacaaa
    23881 accgttggtg tccagacagg tatgaatgcc ttcttttttg caggcgcgga accagtcacg
    23941 aacaaactca gcttgcagga ttgcttcacc gccggatgcg gtaacgccgc cgccggaagc
    24001 gttcataaag tggcgatagg tcaccacctc tctcattaaa tcttcaacgg taacttcttt
    24061 accgccatgc gtgtcccagg tgtcgcggtt atgacaatac aggcagcgca tcaggcagcc
    24121 ctggaaaaag gtgataaagc gaatacccgg accgtctacg gttccacagg attcaaagga
    24181 gtgaatgcga ccaataactg acattgcggt gtttctccag atgtggccca tctgaggccg
    24241 tgttggtgcg cagctcgaag gctacgtcga gtctgttttg gcagtcacct taaagtatag
    24301 atagctgaca aaaaaggctc tcgtgctaaa aaaggcccca ctttcgtgga gcctttattg
    24361 tacgcttttt actgtacgat ttcagtcaaa tctaattaca tagattgagt gaaggtacga
    24421 gtaataacgt cctgctgctg ttctttagtc agcgagttga aacgtactgc gtagccagat
    24481 acacggatgg tcagctgcgg atatttttcc gggttttcca tcgcgtcgag cagcatttca
    24541 cggttcatca cgttaacgtt caggtgctga ccaccttcga tggacgcttc gtggtggaag
    24601 taaccatcca tcagaccagc caggttggtc ttacgaactt cgtcgtcttt acccagtgcg
    24661 ttcggaacga tagagaaggt gtaggagata ccatctttag cgtaagcaaa cggcagttta
    24721 gcaacggaag tcagagaggc tactgcacct ttctggtcac gaccgtgcat cgggttagca
    24781 cccggtccga acggcgcgcc agcacgacga ccgtctgggg tgttaccagt tttcttacca
    24841 tacacaacgt tagaagtgat ggtcagaaca gactgagtcg ggatagcgtc acggtaggtg
    24901 tgcagtttct gaattttctt catgaaacgt tctaccaggt caacagccag gtcatctaca
    24961 cgcggatcgt tgttaccaaa ctgcgggtat tcgccttcga tttcgaagtc gatagccaga
    25021 ccgtcttcgt cacgaatcgg tttaactttc gcatatttga ttgcagacag ggagtcagca
    25081 gcaacggaca gaccagcgat accacacgcc atggtgcgga taacgtcacg gtcgtgcagc
    25141 gccatcagag aggcttcgta gctgtacttg tcgtgcatgt agtggatgat gttcagtgca
    25201 gtgatgtact gtttagccag ccagtccatg aagtgatcca tgcgctccat cacttcatcg
    25261 tagttcagga gatcgccttt gatcggctca gacttcggac caacctgcat tttcagtttt
    25321 tcgtcaacgc cgccgttgat tgcgtacagc atggttttcg ccaggtttgc acgcgcaccg
    25381 aagaactgca tttgtttacc aacgatcatc gggcttacgc agcaagcgat agcgtagtca
    25441 tcgttgttga agtccggacg catcaggtca tcgttctcat actgcagaga agaggtgtcg
    25501 atggacactt tagcagcgaa tttcttgaag ttcagcggca gtttttcgga ccacagaatg
    25561 gtcatgttcg gttccggaga cggacccatg gtgtacaggg tgttcaggaa acggaagctg
    25621 tttttggtaa ccagggtacg accgtcgagg cccataccac cgatagattc ggttgcccag
    25681 atcgggtcgc cagagaacag ttcatcgtat tccggagtac gcaggaagcg aaccatacgc
    25741 agtttcatga ccaggtggtc aaccatttcc tgcgcttctt gttcggtgat cttgccagct
    25801 ttcaggtcac gttcgatgta cacatccagg aaggtggagg tacgaccgaa ggacattgca
    25861 gcaccgttct gagacttaac agcagccagg tagccgaagt aagtccactg gatagcttcc
    25921 tgagcgttgg tagccggacc agagatgtcg tagccgtatt tcgcagccat ttctttcatc
    25981 tgacccagag cgcggtgctg ttcagcgatt tcttcgcgca gacggatagt ctgttccagg
    26041 tttacgccgt tttccagatc agcctgcaga gaggtgaact gagcgtattt gtctttcatc
    26101 agatagtcga taccgtacag cgcaacgcga cggtagtcac cgatgatacg accacggcca
    26161 taagcatctg gcagaccggt cagaacaccg gatttacggc aacgcaggat gtccggagtg
    26221 taaacgtcga acacgccctg gttgtgagtt ttacggtatt cagtgaagat ttttttgatc
    26281 atcgggtcca gttcgcggtt gtacgctttg caggaacctt cgatcatttt gataccaccg
    26341 aacgggataa gagcacgttt cagcggagct tcagtctgca gaccaacaac tttttccaac
    26401 gctttgttga tgtagccagc gtcgtgagag gtgatggtgg aagcaacagc ggtgtcaaag
    26461 tcaactggcg cgtgagtgcg gttttccagt ttaacgcctt ccattacttt gtcccacagg
    26521 gtggtggtcg cttcagtagc gccagccagg aaggactcgt caccctcgta cggagtgtag
    26581 tttttctgaa tgaagtcacg gacgtttact tcattctgcc agtcaccttt ggtaaaacct
    26641 tcccaggctg tggctaactt ttcattaagc tcggacatgt aacacctacc ttcttaagtg
    26701 gattttttat ttactgcgta cttcgacaac cattaatggt gatcgttttc acgcagataa
    26761 atgacccagt atgtcaaccc aaccaacaaa ccaccgccga taatattacc gatcgtaacc
    26821 ggaatcaggt tatcagtgat gaagttcatc acggtcaggt gagaaaaatt ttccggtgca
    26881 gaaccgacag cggtccagaa ttccggagat gcgaagtcgc ggattacaat acccatcggg
    26941 atcataaaca tgtttgcgat actgtgctca aaaccgctgg caacaaacat cgcgaccggc
    27001 agcaccataa tgaacgcttt atccatcagg ctgcggccag aataactcat ccatactgcc
    27061 agacatacca tcaggtttgc caggatacca agacagacgg cctcaataaa agtatggtgc
    27121 actttgtggt cggcggtttg taggacgttt agtccccatt gaccattcgc ggtcatatac
    27181 tcgccggaaa gccacattaa aagtacaaac agcagtgcgc cgaccaggtt gccaaaatag
    27241 acatttagcc agtttttcgc taactgaccc caggtgatgc gcccactcgc cttagcaaca
    27301 acaatcaaca ctgtggaagt aaagagatcg gctccgcaga caacacaaag aatcagcccc
    27361 agagagaagc aaatgccgcc aaccagtttt gccatgccga agggcattgt tcctgtgcca
    27421 gtggttgctg tgatatagaa gacgaatgcg attgagatga aaacaccggc ggtaatcgcc
    27481 agatagaaag tcttaagcgg atgtttcgtt gctttataga cacctgcctc ttcggccact
    27541 ttggccattg ctgcaggaag taaaagatca aaagggttgt cagctttcac actaactctc
    27601 tctttattaa gtcggcgacg agatactaac aaagcattat agatgagaaa ttgatataga
    27661 tcatatctcg cctggcttat aggcccgtaa ctcgcatggt ttttatgcaa atacggagta
    27721 aatatttgat tatccaaata aaaataaatt ttaaaaatta acaaatgagt tgaatt
//
LOCUS       DAZKGF010000051        27694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZKGF010000051 DAZKGF010000000
VERSION     DAZKGF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.051"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            231..473
                     /gene="yjbE"
                     /locus_tag="ACHOQV_002074"
     CDS             231..473
                     /gene="yjbE"
                     /locus_tag="ACHOQV_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFP9630448.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAASGGGDGSNTGTTTTTTTSTQ"
     gene            660..1298
                     /gene="yjbF"
                     /locus_tag="ACHOQV_002075"
     CDS             660..1298
                     /gene="yjbF"
                     /locus_tag="ACHOQV_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFP9630449.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWIEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1295..2032
                     /gene="yjbG"
                     /locus_tag="ACHOQV_002076"
     CDS             1295..2032
                     /gene="yjbG"
                     /locus_tag="ACHOQV_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP9630450.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLARLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            2032..4128
                     /gene="yjbH"
                     /locus_tag="ACHOQV_002077"
     CDS             2032..4128
                     /gene="yjbH"
                     /locus_tag="ACHOQV_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP9630451.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQTKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4175..4453)
                     /locus_tag="ACHOQV_002078"
     CDS             complement(4175..4453)
                     /locus_tag="ACHOQV_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630452.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4667..5077
                     /gene="psiE"
                     /locus_tag="ACHOQV_002079"
     CDS             4667..5077
                     /gene="psiE"
                     /locus_tag="ACHOQV_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFP9630453.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5171..6061)
                     /gene="malG"
                     /locus_tag="ACHOQV_002080"
     CDS             complement(5171..6061)
                     /gene="malG"
                     /locus_tag="ACHOQV_002080"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFP9630454.1"
                     /translation="MAMVQPKSQKTRLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRVTPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(6076..7620)
                     /gene="malF"
                     /locus_tag="ACHOQV_002081"
     CDS             complement(6076..7620)
                     /gene="malF"
                     /locus_tag="ACHOQV_002081"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFP9630455.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7774..8964)
                     /gene="malE"
                     /locus_tag="ACHOQV_002082"
     CDS             complement(7774..8964)
                     /gene="malE"
                     /locus_tag="ACHOQV_002082"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFP9630456.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSDLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            9329..10444
                     /gene="malK"
                     /locus_tag="ACHOQV_002083"
     CDS             9329..10444
                     /gene="malK"
                     /locus_tag="ACHOQV_002083"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFP9630457.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10516..11856
                     /gene="lamB"
                     /locus_tag="ACHOQV_002084"
     CDS             10516..11856
                     /gene="lamB"
                     /locus_tag="ACHOQV_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFP9630458.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            12094..13014
                     /gene="malM"
                     /locus_tag="ACHOQV_002085"
     CDS             12094..13014
                     /gene="malM"
                     /locus_tag="ACHOQV_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFP9630459.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGIRGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            13193..13690
                     /gene="ubiC"
                     /locus_tag="ACHOQV_002086"
     CDS             13193..13690
                     /gene="ubiC"
                     /locus_tag="ACHOQV_002086"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFP9630460.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            13703..14575
                     /gene="ubiA"
                     /locus_tag="ACHOQV_002087"
     CDS             13703..14575
                     /gene="ubiA"
                     /locus_tag="ACHOQV_002087"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFP9630461.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(14730..17153)
                     /gene="plsB"
                     /locus_tag="ACHOQV_002088"
     CDS             complement(14730..17153)
                     /gene="plsB"
                     /locus_tag="ACHOQV_002088"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFP9630462.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPGVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            17324..17692
                     /gene="dgkA"
                     /locus_tag="ACHOQV_002089"
     CDS             17324..17692
                     /gene="dgkA"
                     /locus_tag="ACHOQV_002089"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9630463.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17802..18410
                     /gene="lexA"
                     /locus_tag="ACHOQV_002090"
     CDS             17802..18410
                     /gene="lexA"
                     /locus_tag="ACHOQV_002090"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP9630464.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            18483..19808
                     /gene="dinF"
                     /locus_tag="ACHOQV_002091"
     CDS             18483..19808
                     /gene="dinF"
                     /locus_tag="ACHOQV_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFP9630465.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            19924..20133
                     /gene="yjbJ"
                     /locus_tag="ACHOQV_002092"
     CDS             19924..20133
                     /gene="yjbJ"
                     /locus_tag="ACHOQV_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9630466.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(20175..20690)
                     /gene="zur"
                     /locus_tag="ACHOQV_002093"
     CDS             complement(20175..20690)
                     /gene="zur"
                     /locus_tag="ACHOQV_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFP9630467.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            20846..21841
                     /gene="dusA"
                     /locus_tag="ACHOQV_002094"
     CDS             20846..21841
                     /gene="dusA"
                     /locus_tag="ACHOQV_002094"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFP9630468.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDVDPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            21975..22217
                     /gene="pspG"
                     /locus_tag="ACHOQV_002095"
     CDS             21975..22217
                     /gene="pspG"
                     /locus_tag="ACHOQV_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFP9630469.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(22383..23366)
                     /gene="qorA"
                     /locus_tag="ACHOQV_002096"
     CDS             complement(22383..23366)
                     /gene="qorA"
                     /locus_tag="ACHOQV_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFP9630470.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            23449..24864
                     /gene="dnaB"
                     /locus_tag="ACHOQV_002097"
     CDS             23449..24864
                     /gene="dnaB"
                     /locus_tag="ACHOQV_002097"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9630471.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            24917..25996
                     /gene="alr"
                     /locus_tag="ACHOQV_002098"
     CDS             24917..25996
                     /gene="alr"
                     /locus_tag="ACHOQV_002098"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP9630472.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            26249..27442
                     /gene="tyrB"
                     /locus_tag="ACHOQV_002099"
     CDS             26249..27442
                     /gene="tyrB"
                     /locus_tag="ACHOQV_002099"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFP9630473.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEI
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         6473 a         7018 c         7476 g         6727 t
ORIGIN      
        1 ttgatttcgg aaaatacgca gattaattac ttttattttt attttaagct tatgattttt
       61 agtgctattt atatataagc tgaagcttat atttgatatt cattccaatt atcctaaaac
      121 cccatcacta attccccgcg ccgtaattcg catgctttag ttgtgtatac tcgatcccgc
      181 ccgaaatgtt tttgggtaaa tctccattca ttcaatgaag ggaaattgtt atgaaaaaag
      241 ttctgtatgg catttttgcc atatctgcgc ttgcggcgac ttctgcgtgg gctgcacctg
      301 tacaggtggg cgaagcggca gggtcggcag caacgtcggt ttcggcgggg agttcctccg
      361 cgaccagcgt cagcaccgta agctcggcgg tgggtgtcgc gctcgcggca agcggtggcg
      421 gtgatggttc taataccggg accacaacaa ccacgaccac cagtacccag taataaagta
      481 tgtatcccca aaataattcg agttgcagga aggcgacaag cgagtgagtc cccaggagca
      541 tagataacta tgtgaccggg gtgaacgagt gcggtcaccg catctgcagc ttgaagtatg
      601 aaggggatta accataacca cactccggtg tggttattct gcccctctgg agaagagtcg
      661 tgaagcgacc tgcactcatt cttatctgcc tgctattaca ggcctgttca gccacgacta
      721 aagagctggg caattcactg tgggacagtc tgttcggcac gccaggcgta cagctgacgg
      781 acgatgatat tcaaaatatg ccctacgcca gccagtacat gcagcttaat ggcgggccgc
      841 agttatttgt ggtgctggcc ttcgctgaag acggacaaca aaaatgggtc actcaggatc
      901 aggccactct cgtcacacaa catggtcgtc tggtgaagac tttgcttggc ggcgacaacc
      961 tgattgaagt gaataacctt gccgccgacc cgctgattaa acccgcacaa attgttgatg
     1021 gtgcaacatg gacgcgcacg atgggctgga tcgagtacca gcaggtacgc tacgccaccg
     1081 cacgctcagt cttcaaatgg gatggcaccg ataccgtcaa ggtcggcagc gatgaaaccc
     1141 cggttcgcgt gctggacgaa gaagtctcca ccgaccaggc gcgctggcat aaccgctact
     1201 ggatcgacag cgaagggcaa attcgccagt cggaacagta tctcggcgcg gattatttcc
     1261 cggtgaaaac cactctcatc aaggcggcaa aacaatgatt aaacaaacta ttgtcgcatt
     1321 gattttgagt gtgggggcgt catcggtctt cgcggcagga accgtcaagg tgttcagcaa
     1381 tggcagcagt gaggccaaaa cgctaacggg cgcagagcat ttaatcgatc tggtagggca
     1441 acctcgactg gcaaacagct ggtggcctgg tgcagtgatc agcgaagagc tggcaacggc
     1501 ggcagcattg cggcagcagc aggcgctact ggcacggctg gcagaactgg ccgcagattc
     1561 cagcgctgac gatgccgctg cgattaacgc cttacgccag caaattcagg cgttgaaagt
     1621 gacgggcagg caaaaaatca atcttgatcc cgatatcgtg cgcgttgccg aacgtggtaa
     1681 cccaccgttg cagggcaact acacactgtg ggtcggacca ccaccgtcca ctgtcacgtt
     1741 gttcgggctt atcagccgtc ctggcaagca gccattcact ccaggtcgcg acgtggcgag
     1801 ctatctctcc gggcagaacc tgcttagcgg tgcggatcgc agctacgcgt gggtggttta
     1861 cccggacgga cgcacgcaaa aagcgccggt ggcttactgg aacaagcgtc acgtagagcc
     1921 gatgcccggc agcattattt atgttggcct cgcggactcc gtctggagtg agacacctga
     1981 tgcccttaac gccgacattc ttcagactct gacgcagcgg ataccccaat aatgaaaaaa
     2041 agacatctgc ttagcttact ggcgctgggt attagcacag cttgctacgg cgaaacatat
     2101 cctgcgccca ttggtccgtc gcaatcggat ttcggtggcg taggattatt acaaacgccc
     2161 accgcgcgca tggcgcggga aggggagctg agtctgaact atcgcgataa cgatcagtac
     2221 cgttattact cagcttcagt gcaactcttc ccgtggctgg aaacaacgct gcgctacacc
     2281 gacgtgcgca cccggcagta cagcagcgtc gaagcgttct ctggcgatca aacgtataaa
     2341 gataaagcct tcgatctcaa actgcgtttg tgggaagaga gttactggct gccgcaagtg
     2401 gcggttggtg cgcgggatat cggcggtacg gggctgttcg atgcggaata tcttgtcgcc
     2461 agcaaagcct gggggccatt cgattttacg ctcggcctag gctggggata tctgggcacc
     2521 agcggtaatg tgaaaaatcc gctctgttcg gccagtgaca aatattgcta tcgcgataac
     2581 agctacaaac aagcgggatc tatcgacggc agccagatgt tccacggtcc ggcctcactg
     2641 tttggcggcg tggaatacca gacgccctgg caaccgctgc gcctgaaact ggagtatgaa
     2701 ggcaataatt atcagcagga ttttgccggt aagctggagc aaaaaagtaa gtttaacgtc
     2761 ggggcgattt atcgcgttac cgattgggcc gacgttaacc ttagctatga acgtggcaac
     2821 acttttatgt ttggcgtcac gctgcgcacc aactttaacg atctgcgccc gtcctacaac
     2881 gataacgccc gcccgcaata tcaaccgcaa ccacaggatg ccattttgca gcattcggtg
     2941 gtggcgaatc agttaacgct gttgaaatac aacgccggac tggccgatcc gcagatccag
     3001 acgaaaggcg atacgctgta tgtcaccggc gagcaggtga aatatcgtga ttcgcgtgaa
     3061 gggatcatcc gtgctaatcg gatcgtgatg aacgatctgc cggatgggat caaaacgatc
     3121 cgcattacgg aaaatcgcct taacatgccg caggtgacga cggaaaccga tgtcgccagc
     3181 ctgaaaaatc atctcgccgg agagccgttg ggccacgaaa cgaagctggc gcaaaaacgc
     3241 gtcgagccag tggttccgca gtccaccgag cagggctggt atatcgacaa atcacgcttt
     3301 gatttccata tcgatccggt gttgaaccag tcggtcggtg gcccggaaaa cttttacatg
     3361 tatcagctgg gcgtgatggg aacggcggat ttgtggctga cggaccatct gctgaccacc
     3421 ggcagcctgt ttgctaacct tgccaacaac tacgacaagt ttaactacac caatccgccg
     3481 caggactcgc acttaccgcg cgttcgtacc catgtgcgcg agtatgtgca gaacgatgtc
     3541 tatgtgaata acctgcaagc caactacttc cagcatctgg gcaacggctt ctacggtcag
     3601 gtctacggag gttatctcga aaccatgttt ggcggtgcgg gggcagaagt gttgtatcgc
     3661 ccgctggaca gcaactgggc gtttggtctg gatgccaact acgttaaaca gcgcgactgg
     3721 cgtagtgcaa aagatatgat gaaattcacc gactacagcg tgaaaaccgg acatctgacc
     3781 gcatactgga cgccatcttt tgctcaggat gtgttagtga aagcgagcgt cgggcagtat
     3841 ctggcagggg ataaaggcgg cacgctggag atcgccaaac gctttgatag cggcgtggtg
     3901 gtgggtggct atgccacgat caccaacgtt tcgaaagagg agtacggcga aggggacttt
     3961 accaaaggcg tgtatgtctc tgtaccgctg gatctcttct cgtctggccc gacacgtagc
     4021 cgtgcggcaa ttggctggac gccgctgacg cgtgacggtg gtcagcaact tgggcgtaag
     4081 ttccagttgt atgacatgac cagcgaccgt agcgtcaatt tccgctaagt catggaaaag
     4141 gtgccagttt tcgcactcac tggcaccctc attcttaatg gcctgtcgag ccatcaggaa
     4201 cgcagttcac ccccggtttg cgatctggtg tgtaaaggcg aaaacattct gtcttcctgt
     4261 ccgtcgatat cggttgcagg tctggttgcc tgagcgactg ggccatcatt ggactggaga
     4321 cattgctttg cggtaggtcg gctttatcag cagtaccgag cggaccagca taagcaggaa
     4381 gaacagagac tgataacatc aaagcagcaa aataaggctt catttttacc acctttatca
     4441 ggttacgttt catttgttcc agaggaacat tgtcgatttt tcgcgcattg ctggtggctg
     4501 ggaatcacct gaatgggtga tttttttggc tgtgtgtttt gccagatgag acattgatgg
     4561 gagatgccca cttaattgag ctgaacaaaa catacacaaa aaatatagat ctccgtcaca
     4621 tttttgcgtt atacaggaag ctcgccactg tgaaggaggt actgctatga cgtcactctc
     4681 tcgtccgcgc gtggagttta tatccaccat tttgcagacc gtactcaatc ttggcctgtt
     4741 gtgcctcggc ctgattttgg ttgtcttcct cggcaaagaa acggtgcatc tggctgatgt
     4801 gctgttcgcg ccagaacaaa ccagtaaata tgagctggta gaagggctgg tggtttactt
     4861 tctctatttc gaatttatcg cgctgattgt gaagtacttt cagtccggtt ttcacttccc
     4921 gctacgttac tttgtctata ttgggatcac cgccattgtg cgcttgatca tcgtcgatca
     4981 taaatcgcca ctggatgtgt tgatctactc ggcagcgatc ctgttactgg tgatcaccct
     5041 gtggttgtgt aattcgaaac ggctgaagcg ggagtaaaaa aaggcgctcc gtggagcgcc
     5101 gaataacagt cacaagttgg gataacgtaa gttgagggtg cagcggcata acattggcag
     5161 aacaacatct ttaacctttc acaccacctg ccgtcaggcc gttgaccagc cagcgttgag
     5221 ccagcaagaa gacgatggtg atcggtaatg cagacatcac ggcagcggcg gcaaagtcac
     5281 cccacaggta gttttgcggg ttgaggtatt gctgcatccc cacagccagg gtgtagctgt
     5341 ttacgtcacg cagtaatagc gacgcgaccg gaacttcagt aatggcagcg ataaacgaca
     5401 ggatgaacac aaccgccaga atcggtactg acagcggcaa caggacaagg cggaaggcct
     5461 gccacggtgt cgcaccatcc agcgccgccg cttcttccag cgaactgtcg atggtttcga
     5521 aataaccttt gatggtccag acatgcagcg caatcccgcc cagatacgcg aaaattacgc
     5581 cgccgtgagt attcaggcca atgaatggaa tgtactcacc cagacgatca aacaacgcat
     5641 acaacgcgac cagtgaaagt actgccggga acatctggaa aatcagcatt cctttcagca
     5701 gcgtcgcttt gcctggaaag cgcatacggg cgaaagcgta ggcgcaggtg gtggagagcg
     5761 ccacaatgcc aatcgcggaa atcccggcga cctttaccga gttccacagc catagcagta
     5821 ccggaaacgg cggcggtgta acgcgaccat cagcctgttc cacgctaaaa cctaatgcca
     5881 gtttccagtg atcccaggag atttgctccg ggatcaggct gccggtcgca aagttcccct
     5941 ggcgcagtga gatagcgacg accatcagta gcgggaacat aatcgccgcg ataaaaagta
     6001 gcagtagcag gtgagtaata aacaaacgtg ttttttgcga tttcggttgg accattgcca
     6061 tttttgttat ctcccttaat caaacttcat tcgcgtggct ttcaggttca ctatcgccag
     6121 cgcacccacc agcaggaaga tcagcgtggc aattgctgcc gccagaccga agtcctgacc
     6181 cccgccgcct tcaaaagcga tgcggtaggt gtagttaaca agcaggtcgg tataaccggc
     6241 tggcgtggtc gtgccaagac gatccgggcc gccgttggtt aacagttgaa tcagcacgaa
     6301 gttgttaaag ttaaaggcga agctggcgat catcagcggc gtcagcggtt taatcagcag
     6361 cggcagcgta atcttaaaga agttctggaa cggacctgcg ccatccattg ctgaggcttc
     6421 atacaaatcg tccggaatcg ctttcagcaa gcccatgcag aggatcatca tgtacggata
     6481 acccagccag gtattgacga taatcagcat cgttcgggcg gtggtcggat cgctgaacca
     6541 ggcgggcttc acgccaaaca gcgcgctcaa catcatgttg atctcaccga agctctggtt
     6601 aaacaaccct ttgaaaatca agattgaaat gaacgatggc accgcgtagg gcagaatcag
     6661 caggacgcga tagaccgctt tgccgcgcaa cgcttcccac tgcaccagac acgccagaac
     6721 catgccgacc gccaccgtta aaaagacagt gatcagcgag aacaccacgg tccagacgaa
     6781 aatggcgagg aacggtttct gaatgccttc gtcggtgaag acgcgggtaa agtttttcca
     6841 gccagtggtc acggtgtaac cggggcttag cttttcatcg ccccagttgc cgtcggcggt
     6901 aatggactgg taaaagccaa tttggttatt cggacgatat ttcacgccgc tctgattatt
     6961 cgtcaacgtg ccgtcaccgt cgagtgtgta gagcggctgc gtgccagaaa actggcgcag
     7021 ggagctcatc atcactttgt tgccatccgg cagaatggcg gtaatgtcac tcagcgcctg
     7081 acgattctgg gtaatcacgc gtagattcgc gcgttcgcct tcgggctggg cggtcgtttc
     7141 tttcagttgc agtttttgct cgccgccaaa tttaaaagcg tcggaaaggt aatttttgcc
     7201 ggtttcgccg tcgctgagcg ccagttgcca ctcatcgccc gccgggtaaa gaccaaagtt
     7261 ataggttttg cctgcttgcc aggagcgatc taacaacact tcctgtgcac gttcaaaagt
     7321 cagctggtta gtgctgctgt agttggtgaa ggcaatggcg atggtgcaga ccagagggaa
     7381 gaggacgaat aatcccattc cagccattcc cgggtaaaca tagcgccagg cgtaggcttt
     7441 acgattggcg aaaatataca gccccgctga actcaatatc agcgtggtaa tggcgaacag
     7501 gtattcccct tgtgcgtaca ttaaaacaac aaggtaaccc accagcaggc cgagcagacc
     7561 taacactgac catttcagcg cgtcgctttg ccaccaatgt ttctttttaa tgacatccat
     7621 ggggttcttc ctcattccag gacggataag gctttcacgc cttatccgac aacaactgcc
     7681 tgatgcgacg ctgacgcgtc ttatcaggcc tacatacgtt tcaatttcgt aggccggata
     7741 aggcgtttac gccgcatccg gcatttcaca gcattacttg gtgatacgag tctgcgcgtc
     7801 tttcagggct tcatcgacag tctgacgacc gctggcggcg ttgatcaccg cagtacgcac
     7861 ggcataccag aaagcggaca tctgcgggat gttcggcata atttcacctt tctgagcgtt
     7921 atccatggtg gcggcaatac gtggatcttt cgcaagttca tcctgatagg acttcagcgc
     7981 tactgcaccc agcggtttgt ctttattgac cgcttccaga ccttcgtcag tcagcagata
     8041 gttttcgagg aactctttcg ccagctcttt gttcggactg gcggcgttaa tacctgcgct
     8101 cagcacgcca acgaacggtt tagatggttg acctttgaag gtcggcagta ccgttacacc
     8161 ataattcact ttactggtgt cgatgttgga ccatgcccac gggccgttga tagtcatcgc
     8221 tgtttcgcct ttattaaagg cagcttctgc gatggagtaa tcggtgtctg cattcatgtg
     8281 tttgttttta atcagatcaa ccaggaaggt tagacccgct ttcgcgccag cgttatccac
     8341 gcctacgtct ttaatgtcgt acttgccgtt ttcatacttg aacgcataac ccccgtcagc
     8401 cgcaattagc ggccaggtga agtatggttc ttgcaggttg aacatcagcg cgctcttacc
     8461 tttcgctttc agttctttat ccagcgccgg gatctcttcc caggtttttg gcgggttcgg
     8521 tagcagatct ttgttataaa tcagtgataa cgcttcgaca gcgatcgggt aagcaatcag
     8581 cttgccgttg taacgtacgg catcccaggt aaacggatac agcttgtcct ggaacgcttt
     8641 gtccggggtg atttcagcca acaggtcaga ttgagcgtag ccgccaaagc ggtcgtgcgc
     8701 ccagaagata atgtcagggc catcgccagt tgccgcaacc tgagggaatt tctcttccag
     8761 tttatccgga tgctcaacgg tgactttaat tccggtatct ttctcgaatt tcttaccgac
     8821 ttcagcgaga ccgttatagc ctttatcgcc gttaatccag attaccagtt taccttcttc
     8881 gattttggcg agagccgagg cggaaaacat catcgtcgtt aatgcggata atgcgaggat
     8941 gcgtgcacct gtttttattt tcataatcta tggtccttgt tggtgaagtg ctcgtgaaaa
     9001 cacctaaacg gactctagtt tctttatacg gcaacctctt tccatcctcc ttgcccctac
     9061 gccccaccgt tgctttgtgt gatctctgtt acagaattgg cggtaatgtg gagatgcgca
     9121 cataaaatcg ccatgatttt tgcaagcaac atcacgaaat tccttacatg acctcggttt
     9181 agttcacaga agctgcgttc tcatcctccc gcctcctccc ccataaaaaa gccagggggt
     9241 ggaggattta agccatctcc tgatgacgca tagtcagccc atcatgaatg ttgctgtcga
     9301 tgacaggttg ttacaaaggg agaagggcat ggcgagcgta cagctgcaaa atgtaacgaa
     9361 agcctggggc gaggtcgtgg tatcgaaaga tatcaatctc gatatccatg aaggtgaatt
     9421 cgtggtgttt gtcggaccgt ctggctgcgg taaatcgact ttactgcgca tgattgccgg
     9481 gcttgagacg atcaccagcg gcgacctgtt catcggtgag aaacggatga atgacactcc
     9541 gcctgcagaa cgcggcgttg gtatggtgtt tcagtcttac gcgctctatc cccacctgtc
     9601 agtagcagaa aacatgtcat ttggcctgaa actggctggc gcgaaaaaag aagtgattaa
     9661 ccagcgagtt aaccaggttg cggaagtgct gcaactggcg catttgctgg atcgcaaacc
     9721 gaaagcgctc tccggtggtc agcgtcagcg tgtggcgatt ggccgtacgc tggtagccga
     9781 gccaagcgta tttttgctcg atgaaccgct ctccaacctc gatgctgcac tgcgtgtgca
     9841 aatgcgtatc gaaatctccc gtctgcataa acgcctgggc cgcacaatga tttacgtcac
     9901 ccacgatcag gtcgaagcga tgacactagc cgacaaaatc gtggtgctgg acgccggtcg
     9961 cgtggcgcag gttgggaaac cgctggagct gtaccactat ccggcagacc gttttgtcgc
    10021 cggatttatc ggttcgccaa agatgaactt cctgccagta aaagtgaccg ccactgcaat
    10081 cgatcaagtg caggtggagc tgccgatgcc aaatcgtcag caagtctggc tgccggttga
    10141 aagccgtgat gtccaggttg gagccaatat gtcgctgggt attcgcccgg aacatctact
    10201 gccgagtgat atcgctgacg tcatccttga gggtgaagtt caggtcgtcg agcaactcgg
    10261 caacgaaacc caaatccata tccagatccc ttccattcgt caaaacctgg tgtaccgcca
    10321 gaacgacgtg gtgttggtag aagaaggtgc cacattcgct atcggcctgc cgccagagcg
    10381 ttgccatctg ttccgtgagg atggcactgc atgtcgtcga ctgcataagg agccgggcgt
    10441 ttaagcaccc cacaaaacac acaaagcctg tcacaggtga tgtgaaaaaa gaaaagcaat
    10501 gactcaggag atagaatgat gattactctg cgcaaacttc ctctggcggt tgccgtcgca
    10561 gcgggcgtaa tgtctgctca ggcaatggct gttgatttcc acggctatgc acgttccggt
    10621 attggctgga caggtagcgg cggtgaacaa cagtgtttcc agactaccgg tgctcaaagt
    10681 aaataccgtc ttggcaacga atgtgaaact tatgctgaat taaaattggg tcaggaagtg
    10741 tggaaagagg gcgataagag cttctatttc gacactaacg tggcctattc cgtcgcacaa
    10801 cagaatgact gggaagctac cgatccggcc ttccgtgaag caaacgtgca gggtaaaaac
    10861 ctgatcgaat ggctgccagg ttccaccatc tgggcaggta agcgcttcta ccaacgtcat
    10921 gacgttcata tgatcgactt ctactactgg gatatttctg gtcctggtgc cggtctggaa
    10981 aacatcgatg ttggcttcgg taaactctct ctggcagcaa cccgttcctc tgaagctggt
    11041 ggttcttcct ctttcgccag caacaatatt tatgactata ccaacgaaac cgcgaacgac
    11101 gttttcgacg tgcgtttagc gcagatggaa atcaacccgg gcggcacatt agaactgggt
    11161 gtcgactacg gtcgtgccaa cctgcgtgat aactatcgtc tggttgatgg cgcatcgaaa
    11221 gacggttggt tattcactgc tgaacatact cagagtgtcc tgaagggctt taacaagttt
    11281 gttgttcagt acgctactga ctcgatgacc tcgcagggta aaggtctgtc gcagggttct
    11341 ggcgtcgcgt ttgataacga aaaatttgcc tacaatatca acaacaacgg tcacatgctg
    11401 cgtatcctcg accacggtgc gatctccatg ggcgacaact gggacatgat gtacgtgggt
    11461 atgtaccagg atatcaactg ggataacgac aacggcacca agtggtggac cgtcggtatt
    11521 cgcccgatgt acaagtggac gccaatcatg agcaccgtga tggaaatcgg ctacgacaac
    11581 gtcgaatccc agcgcaccgg cgacaagaac aatcagtaca aaattaccct tgcacaacaa
    11641 tggcaggctg gcgacagcat ctggtcacgc ccggctattc gtgtcttcgc aacctacgcc
    11701 aagtgggatg agaaatgggg ttatgactac accggtaacg ccaataccaa cactaacttc
    11761 ggcaaagccg ttcctgctga tttcaacggc ggcagcttcg gccgtggtga cagcgacgag
    11821 tggaccttcg gtgcccagat ggaaatctgg tggtaatagc aaaacctggg ccggataagg
    11881 tgcttgcgcc gcatccggca accaacgcct gatgcgacgc ttgcgcgtct tatcaggcct
    11941 acaactacaa ccgctgccga aagtaggccg gataaggcgt ttacgccgca tccggcataa
    12001 aaacagcttt aaggggcgaa agcccctctg attatcgggt ttagcgcgct attgcctggc
    12061 taccgctgag ctccagattt tgaggtgaaa acaatgaaaa tgaataaaag tctcatcgct
    12121 ctctgtttat cagcagggtt gctggcaagc gcacctggaa tcagccttgc cgatgttaac
    12181 tacgtaccgc aaaacaccag cgacgcgcca gccattccat ctgccgcgct gcaacaactc
    12241 acctggacac cggtcgatca atctaaaacc cagaccaccc aactggcgac tggcggccaa
    12301 caactgaacg ttcccggcat cagaggtccg gttgctgcgt acagcgtccc ggcaaacatt
    12361 ggcgaactga ccctaacgct gaccagcgaa gtgaacaaac aaactagcgt atttgcgccg
    12421 aacgtgctga ttcttgatca gaacatgacc ccatcagcct tcttccccag cagttatttc
    12481 acctaccagg aaccaggcgt gatgagtgca gatcgtctgg aaggcgttat gcgcctgaca
    12541 ccggcgttgg ggcagcaaaa actttatgtt ctggtcttta ccacggaaaa agacctccag
    12601 cagacgaccc aactgctcga cccggctaaa gcctatgcca aaggcgtcgg taactcgatc
    12661 ccggatattc ctgacccggt tgctcgtcat accaccgatg gcttactgaa actgaaagtc
    12721 aaaacgaact ccagttctag cgtgttggtc gggcctctat ttggttcttc cgctccagct
    12781 ccggttacgg taggcaacac ggcggcacca gctgtggctg cacccgctcc ggcaccggtg
    12841 aagaaaagcg agccgatgct caacgacacg gaaagttatt ttaataccgc gatcaaaaac
    12901 gctgtcgcga aaggtgatgt tgataaggcg ttaaaactgc ttgatgaagc tgaacgcctg
    12961 gggtcgacat ctgcccgttc cacctttatc agcagtgtaa aaggcaaggg gtaattacgc
    13021 cccacagtgc tgattttgca acaactggtg cgtctcctgg cgcacctttt tttctcctcc
    13081 tgtactattg ttgcgttttt gttgcgcatt agttcactta atttgcttta catctcccgt
    13141 aaatattttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
    13201 ccccgcgtta acgcaactgc gtgcgctgcg ctattttaca gagatccctg ccctggagcc
    13261 gcaactgctc gactggctgt tgctggaaga ttccatgaca aaacgttttg aacagcaggg
    13321 aaaaacggtg agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
    13381 agaactgcca ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
    13441 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
    13501 ggagctggcg ttacaaaaat taggtaaaac gccgttagga cgctatctgt tcacatcatc
    13561 gacattaacc cgggacttta ttgagatagg ccgtgatgcc ggactgtggg ggcgacgttc
    13621 ccgcctgcga ttaagcggta aaccgctgtt gctaacagaa ctgtttttac cggcgtcacc
    13681 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
    13741 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
    13801 atgggcgttg tgggtggcga caccgggcgt tccccagctc tggatcctgg cggtgtttgt
    13861 cgcgggcgtc tggctgatgc gcgctgctgg ttgcgtggtg aatgattatg ctgaccgcaa
    13921 gtttgatggt catgttaagc gcacggcgaa ccgaccactc cccagcggcg cggtaacgga
    13981 gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt tcatttttac tggtgctgac
    14041 gctgaatacg atgactattc tgttgtcgat tgccgcgctg gcgctggcgt gggtataccc
    14101 gtttatgaag cggtataccc atctaccgca agtggtgctg ggcgcggcgt ttggctggtc
    14161 gattccaatg gcttttgctg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
    14221 cctcgccaat attctctggg cggtggcata cgacacgcag tatgcgatgg ttgaccgtga
    14281 tgatgatgtg aagattggca ttaaatccac cgcaatcctg ttcggccaat acgataaatt
    14341 gattattggt attttgcaga ttggcgtact ggcgctgatg gcgattatcg gtgagttaaa
    14401 tggcttaggc tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca
    14461 acaaaaactg attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa
    14521 ctatgttggt ctggtactgt ttttagggct ggcaatgagt tactggcatt tctgatgatg
    14581 taaaaaagcc ggatgatcat ccggcttcct tctgggttgc ctgatgcgct gcgcttctca
    14641 ggcctacaca acatatcgca atttattgaa tttgcagatc atggtaggcc gaataaggcg
    14701 tttgcgccgc atccggcaat tctccctgat tacccttcgc cctgcgtcgc actctcaatc
    14761 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gatacacctt catcgtttct
    14821 gccggttcgg cgtcgccgct atcgctgata tacccttcat cacgcagtgt cagcaccaga
    14881 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga tgccgtgcag cacggagaga
    14941 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttggcg
    15001 ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc
    15061 agctgtagcg tgcgagaatg cgccgggttg atatgcaact catcatcttg cagggtaatc
    15121 agcccctgac gttgcatctc atttgccagt gcatcaataa cgcccggcaa ctcgtcgcga
    15181 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
    15241 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc
    15301 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc
    15361 agaatgatga tgtcgccgat cgtgtctttc tcgacttcaa acttgttcat ttgcagcgcg
    15421 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggcacg
    15481 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtgagtgag
    15541 cgctgacgtg acgccagaag cgcagtacaa cacaggttca tggcgtttgc cgcgcctgcg
    15601 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccatgcc
    15661 ggacgcaccg cttcgatggg atcgatagat tcacgccagt caggtacatg ctggttaagg
    15721 taggtcatca acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc
    15781 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt
    15841 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
    15901 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
    15961 ttcggatcca gcaaacgccc tgtacgggaa cgaccgcctt ccacgaaata ctcgacggaa
    16021 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata gagcttattg
    16081 cctttaaacg tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggcaggc
    16141 cagaaattca ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtgatacagc
    16201 acgtaagaaa gcagcaggta gtccatgtgg ctgcggtggc aaggcacata caccagctca
    16261 tgaccgtcgt gggccagctg acgaacgcgc tcggcgttat ggacgttgat gccctggtaa
    16321 aggcggttcc aggtgaagcc cagaatacgg tcagtcaggc ggatcatctc gtaagagaaa
    16381 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
    16441 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
    16501 ttaaacagat cctgacgagc aggcagacgt gggcctacgg cagccagacg ttgacgggca
    16561 aagtgcatac gcgccacgcg cgccagtttc tgcgcgatag ttttatccgt gccgtgttca
    16621 tccgccatac ggcgcagcga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
    16681 cacagtacag cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gttcacttcg
    16741 cctttttcac gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc
    16801 agatttgggt tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct
    16861 ttcggcgtgt aataggtgaa cacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
    16921 agcgtgccgt cgatttccag cggctctaac gggtcaggca ggtcatgtgc cagacactgg
    16981 gcgcgcaacg tcagcaagtc agctttcgag ttgtacggta aaacgtacat aattggacga
    17041 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg
    17101 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
    17161 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
    17221 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
    17281 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
    17341 tggattcacc cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
    17401 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
    17461 ctgctggctg gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt
    17521 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
    17581 ataccatgag ctttccggac gcgctaaaga tatgggatcc gccgcggttc tgattgcgat
    17641 tatcgtcgcc gtgattacct ggtgcatcct gttatggtcg cattttggat aacccttcca
    17701 gatttcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
    17761 atatactcac agcataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca
    17821 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
    17881 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
    17941 tgaaggcgct ggcacgcaaa ggcgttattg aaattgtttc cggcgcatca cgcgggattc
    18001 gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac
    18061 cgcttctggc gcaacagcat attgaaggtc attatcaggt cgatccttcc ttattcaagc
    18121 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatc ggcattatgg
    18181 atggcgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
    18241 cacgtattga tgacgaggtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
    18301 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
    18361 ccattgaagg gctggcggtt ggagttattc gcaacggcga ctggctgtaa catatctctg
    18421 agaccgcgat gccgcctggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
    18481 gcatggcatt cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt
    18541 tctccaatat caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg
    18601 atagtccggt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
    18661 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
    18721 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggctg
    18781 gggcgttaat tgcgctgctg cgtacgccga ttatcgatct ggcgctgcat attgttggcg
    18841 gcagcgaagc ggtgctagaa caggcgcgac gctttcttga aatccgctgg ttaagtgcac
    18901 cggcgtcgct ggcgaatctg gtattacttg gttggttact cggtgtgcaa tatgcccgtg
    18961 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
    19021 tgatggggct gcatatgaac gtgcagggcg cggcgctggc gacggttatt gcggaatatg
    19081 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatctccg
    19141 gcgaaatgct gaaaactgcc tggcgaggaa acttccgtca cttgctggcg cttaaccgcg
    19201 atatcatgct gcgctcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggcg
    19261 cgcgactggg gagtgacatt atcgctgtta acgcggttct gatgacgtta ctcaccttta
    19321 ccgcctatgc gctggatggt tttgcctacg cggttgaagc gcattccggt caggcgtacg
    19381 gtgcgcgcga tggtagccag ttactggatg tctggcgggc agcgtgccgc cagtcgggga
    19441 tcgtagcgtt actgttttcg gtggtttatt tgctggcagg ggaacacatc attgcgttgc
    19501 tgacgtcgtt aacccagatt cagcagctgg ctgaccgcta tcttatctgg caggtgattt
    19561 tgccgttggt cggcgtctgg tgttatctgc tcgacggcat gtttataggt gcaacgcgcg
    19621 ccgccgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
    19681 cgctgccgtg gctggggaat catggtttgt ggctggcatt aaccgtcttt ctggctttgc
    19741 gcgggctttc tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg
    19801 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
    19861 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
    19921 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
    19981 caatggggaa aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
    20041 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggttgat
    20101 agttgggaaa aacgccatga ttaccgctgg taattaatcc ctcctgcccg acatgtacaa
    20161 ggatgtacac ccttttagcg aggtttcttt ttcacctgaa tagagtggtc atgatggcac
    20221 tgttcgggat gacgacacgc ttctacttct acacatgctg aacataatcc atgtgcttca
    20281 ataacattat gccgcagggc aaaacccatt tttgccgcca gcgtatgcat gatgtcttcc
    20341 acgccttcag cacattcttc tttcaccgcg ccgcagcgat cgcaaataaa catggctgaa
    20401 gtatgggtgg gctgatcgaa cagatgacag agcacataac tgttggtgga ttccacctta
    20461 tgcacaaaac cttgttcaag cagaaactcc agcgcgcgat aaaccgttgg cggcttggct
    20521 tgcggttcag cttcgcgcag caaatcaagc agatcataag cgctgatagc gccatcctgc
    20581 agactcatca ggcgcaatac ttccaggcgc tgtggggtca ggcgcacatt acgctgtgcg
    20641 cagatttttt cagcctgcgc taataactcc tgcgttgtgg tcttttccat tgggcacctc
    20701 gacagtgggg ttaagaatgt ccttacttta ccatagttct cgcttaaacg ccgagaacag
    20761 ggtgtcatgg ctgtcacatt gcctgatcgc atattcgtta tgctatagta gcgcccccaa
    20821 tttaccagat gcttaaaaag tcgccatgcc agcagaatca cacactgttt atcccgctta
    20881 ccgtttctcg attgccccga tgcttgactg gacggacaga cattgccgct atttcttgcg
    20941 tctgctttcc cgcaatacgt tgctgtatac cgaaatggtg actacagggg cgattattca
    21001 cggtaaaggt gattacctgg cgtacagtga agaagaacat ccggtagcgt tgcaactcgg
    21061 gggtagcgat ccggcggcgc tggcacagtg tgcgaagctg gcagaagcgc gcggatatga
    21121 tgagatcaac ctgaatgtcg gctgcccgtc tgaccgggtg cagaacggca tgtttggtgc
    21181 gtgtctgatg ggtaatgcgc agctggttgc cgactgcgtg aaagcgatgc gcgatgtggt
    21241 gtcgattccg gtgacggtga aaacgcgtat tggcatcgat gatcaggaca gctatgaatt
    21301 tctctgcgat ttcatcaaca ccgtttccgg caaaggtgag tgtgagatgt tcatcatcca
    21361 cgcacgtaaa gcctggcttt cggggttaag cccgaaagaa aaccgtgaaa tcccgccgct
    21421 cgattatccg cgtgtgtatc aactgaagcg tgactttccg catctgacga tgtcgattaa
    21481 cggtggcatc aagtcgctgg aagaggctaa agcgcatttg caacatatgg atggcgtgat
    21541 ggtcgggcgc gaagcgtatc agaatccagg cattctggcg gcggtagacc gagagatctt
    21601 tggttcttcg gataccgatg tcgatccggt ggcggtggtg cgtgccatgt atccgtacat
    21661 tgagcgtgaa ctcagccagg ggacgtatct cggccatatt acccggcata tgctgggctt
    21721 gttccagggt attcctggcg cgcggcagtg gcggcgttat ttaagtgaaa atgcccataa
    21781 agcgggtgct gacattaacg tgctggaaca cgcgctcaaa ctggtggcgg ataagcgtta
    21841 acttttcacc aaaaagtagt caaattcacc acgccctgcg caccgtcgcg gggcgttttg
    21901 ctgttaaatc aatagattat ttttggcatg attcttgtaa tgcctgcaag agatttcata
    21961 tttgggagag catcatgctg gaactacttt ttgtgattgg cttttttgtc atgctgatgg
    22021 tcaccggcgt ttcgttgctg ggcattatcg ccgcgctggt tgtggcgacg gccattatgt
    22081 tcctcggcgg tatgctggca ttgatgatta agttgctgcc gtggttactc ctggcgattg
    22141 cggtggtgtg ggttattaag gcgattaaag caccaaaagt gccgaaatat cagcgttatg
    22201 accgctggcg ttactaaggg attgtgcgga tgatcacaac ctaaggtttt atccttagaa
    22261 caaaatagga attgataatc aaatctgtca ctattgcgcc tctaacagat tcatcgtgct
    22321 gtaccctaca tacagccgaa ctataaaaag aaagggcttc ccagatggaa gccctatttc
    22381 ttttatggaa tcaacaggct ggaaccttgc gtcgcccggc tttccagaat ctcatgcgca
    22441 cgctgtgcat ccttaagcgg atatttctgc tgctcggcga catcgacctt aatcacaccg
    22501 ctggcaatca aagagaacag ttcattactg gcctcggtta attcctcccg tgtggtgata
    22561 tagccttgta gggaagggcg tgtcacatac aacgagcctt tctgattgag aatgcctaag
    22621 ttcacaccgg taaccgcacc tgatgagttg ccaaagctca ccattaagcc gcggcgttgc
    22681 aggcaatcca gcgaccgttc ccaggtgtct ctgcccacgg aatcgtacac cacgcgcact
    22741 ttcttgccac cggtgatctc ttttaaccgc tcgaccagat tctcttcacg atagttaata
    22801 acctgccatg cgcccgcttt tagcgcactc tgcgcttttt gcgcggttcc tacggtgccg
    22861 ataagtttcg cgccaagggc ttttgcccac tggcaggcaa tcaagccaac gccgccagcc
    22921 gctgcgtgga acaggaactg ctcatcgggt ttaatttcat aggttttgcg cagcagataa
    22981 taaaccgtta agcctttcag gaaggatgcc gcagcttgct caaaagaaat tgccgcaggc
    23041 agaatcgccg ctttatccgc attaatgtta tgcacagagc tgtaagcgcc taatgccgat
    23101 tgtgcataga ctacgcgatc gcctgcctta atatgcttta caccactgcc gactttactc
    23161 acgatgcctg ccgcttcggt gcctaatccg ctgggtagcg atggcggcgg gtaaaggccg
    23221 ctgcggatat acgtgtcgat aaaattgatg ccgatggctt tattttcgac ctggatttca
    23281 ttctccgccg gatcggcagg agtgaactct acggcttgaa gtacttccgg gccaccgtgc
    23341 ttgtgaaatt caattcgtgt tgccatgtgt cctccagaac gtatcgtcag ggtctgcttc
    23401 atatggtaaa gtttcgaccc attctttatc tcggtaactc cattcactat ggcaggaaat
    23461 aaacccttca acaaacagca ggctgaaccc cgcgaacgcg atccacaagt tgccgggctg
    23521 aaagtgcctc cgcactcgat cgaagcggag cagtcggtgt tgggcggttt aatgctggat
    23581 aacgaacgct gggatgatgt agctgagcgt gtggtggcag atgattttta cacccgccca
    23641 caccgtcata tctttactga aatggcgcgt ttgcaggaaa gcggtagtcc tatcgatctg
    23701 attacccttg cggaatcgct ggaacgccag gggcaactcg atagcgtcgg tggttttgct
    23761 tatctggcag agctgtcaaa aaatacgcca agtgcggcga acatcagtgc ttatgctgac
    23821 atcgtgcgtg aacgtgccgt tgttcgcgag atgatttcgg ttgcgaatga gattgccgaa
    23881 gccggttttg atccgcaggg gcgcaccagc gaagatctgc tggacctggc tgaatcccgc
    23941 gtctttaaaa ttgccgaaag tcgtgcaaac aaagacgaag ggccgaagaa catcgctgat
    24001 gtgctcgacg ccacggtggc gcgtattgag cagttgtttc agcagccaca cgatggcgtt
    24061 accggggtaa acaccggtta tgacgatctc aacaaaaaaa ccgctggctt gcagccgtcg
    24121 gatttgatca tcgtcgccgc gcgtccgtcg atgggtaaaa caacatttgc gatgaacctc
    24181 gtcgaaaacg cggcgatgtt gcaggataaa ccagtactta tcttctcgct ggagatgcca
    24241 tcagaacaga ttatgatgcg ttctctggcg tcgctgtcgc gcgttgacca gactaaaatc
    24301 cgtaccgggc agctcgatga tgaagactgg gcgcgcattt ccggcaccat gggtattttg
    24361 ctcgaaaaac gcaatatcta tatcgatgac tcctccggcc tgacgccaac ggaagtgcgt
    24421 tcccgcgcgc gccgtattgc ccgtgaacac ggcggcatcg ggcttatcat gatcgactac
    24481 ctgcaactga tgcgcgtacc ggcgctttcc gataaccgta cgctggaaat tgcagaaatc
    24541 tcccgctcgc tgaaagcact ggcgaaagaa ttgaacgtgc cggtggtggc gctgtcccag
    24601 ttgaaccgtt ctctggaaca acgtgccgac aaacgcccgg tcaactccga cctgcgtgaa
    24661 tctggctcta tcgagcagga tgcggacttg atcatgttta tctatcgtga tgaggtgtat
    24721 cacgaaaaca gtgatttaaa aggcatcgcg gaaattatta tcggtaaaca acgtaacggc
    24781 ccaatcggga cggtacgcct gacctttaac ggtcaatggt cgcgcttcga caactatgcg
    24841 gggccgcagt acgacgacga ataataatta ttttatgaat taggtaatta aagcaaacac
    24901 ttatcaagga acacaaatgc aagcggcaac tgttgtgatt aaccgccgcg ctctgcgaca
    24961 caacctgcaa cgtcttcgtg aactggctcc tgccagtaaa atggttgcgg tggtgaaagc
    25021 gaacgcttat ggtcacggtc ttcttgagac cgcgcgaacg ctccccgatg ctgacgcctt
    25081 tggcgtagcc cgtctcgaag aagctctgcg actgcgtgcg gggggaatca ccaaacctgt
    25141 actgttactc gaaggctttt ttgatgccag agatctgccg acgatttctg cgcaacattt
    25201 tcataccgcc gtgcataacg aagaacagct ggctgcgctg gaagaggcta gcctggacga
    25261 gccggttacc gtctggatga aactcgatac cggtatgcac cgtctgggcg taaggccgga
    25321 acaggctgag gcgttttatc atcgcctgac ccagtgcaaa aacgttcgtc agccggtgaa
    25381 tatcgtcagc cattttgcgc gcgcggatga accaaaatgc ggcgcaaccg agaaacaact
    25441 cgctatcttt aatacctttt gtgaaggcaa accaggtcaa cgttccattg ccgcgtcggg
    25501 tggcattctg ctgtggccac agtcgcattt tgactgggtg cgcccgggca tcattcttta
    25561 tggcgtctcg ccgctggaag atcgttccac cggtgccgat tttggctgtc agccggtaat
    25621 gtcattgacc tccagcctga ttgctgtgcg tgagcataaa gccggagagc ctgtcggtta
    25681 tggtggaacc tgggtaagcg aacgtgatac ccgccttggc gtagtcgcga tgggctatgg
    25741 cgatggttat ccgcgcgccg cgccgtccgg tacgccagtg ctggtgaacg gtcgcgaagt
    25801 gccgattgtc gggcgcgtcg cgatggatat gatctgcgta gacttaggtc cacaggcgca
    25861 ggacaaagcc ggggacccgg tcattttatg gggcgaaggt ttgcccgtag aacgtatcgc
    25921 tgaaatgaca aaagtgagcg cttacgaact tattacgcgc ctgacttcaa gggtcgcgat
    25981 gaaatacgtg gattaatcgt tctgtaatat ttgattgtct gtgccggatg cggcgtgaat
    26041 gccttatccg gccaataaaa tcttaccaat tcaataagtt gatattcttt catgctctta
    26101 taaaggtcgt gcatctggcg gatgtacgtt tgtcatgagt ctcactctgt tgctaattgc
    26161 cattcgcccc tgaacatcct ctcgatcttc gccttcttcc ggtttattgt gttttaacca
    26221 cctgcccgta aacctggaga accatcgcgt gtttcaaaaa gttgacgcct acgctggcga
    26281 cccgattctt acgcttatgg agcgttttaa agaagaccct cgcagcgaca aagtgaattt
    26341 aagtatcggt ctgtactaca acgaagacgg aattattcca caactgaaag ccgtagcgga
    26401 tgcggaagcg cgcctgaatg cgcagcctca tggtgcttcg ctttatttac cgatggaagg
    26461 gcttaacagc tatcgccatg ccattgcgcc gctgctgttt ggtgcggacc atccggtact
    26521 gcaacaacag cgcgtagcaa ccattcaaac ccttggcggc tcaggggcat tgaaagtggg
    26581 tgcagatttc ctgaaacgct acttcccgga atcaggcgtc tgggtcagcg atcctacctg
    26641 ggaaaaccac gtagcaatat tcgccggggc tggattcgaa gtgagcactt acccctggta
    26701 tgacgaagcg actaacggcg tgcgctttaa tgacctgttg gcgacgctga aaacattacc
    26761 tgcccgcagt attgtgttgc tgcatccatg ttgccacaac ccaacgggtg ccgatctcac
    26821 taatgaccag tgggattccg tgattgaaat tctcaaagcc cgcgagctta ttccattcct
    26881 cgatattgcc tatcaaggat ttggtgccgg tatggaagag gatgcctacg ccattcgcgc
    26941 cattgccagc gctggattac ccgctctggt gagcaattcg ttctcgaaaa ttttctccct
    27001 ttacggcgag cgcgtcggcg gactttctgt tctgtgtgaa gatgccgaag ccgcaggccg
    27061 cgtactgggg caattgaaag caacagttcg ccgcaactac tccagcccgc cgaattttgg
    27121 tgcgcaggtg gtggctgcgg tgctgaatga cgaggcattg aaagccagct ggctggcgga
    27181 agtagaagag atgcgtactc gcattctggc gatgcgtcag gaactggtga aggtattgag
    27241 cacagagata ccagaacgca atttcgatta tctgcttaat cagcgcggta tgttcagtta
    27301 taccggttta agtgccgctc aggttgaccg actacgtgaa gaatttggtg tctatctcat
    27361 cgccagcggt cgcatgtgtg tcgccgggtt aaatacggca aatgtacaac gtgtggcaaa
    27421 ggcgtttgct gcggtgatgt aatgcaggaa agcaggctgg agttatccag cctgcagtga
    27481 aattaaactg tcgtcgcttt cactcttgct ttatagatga tttttttgat gccatcgttc
    27541 tacgtgagag gtaataaaac gttgttagtt ctaacttgtt aagtttatcc caatgttctg
    27601 gagtactttt ccctgtttta ccgtgtggaa taataatttt tgcacatcta accattatta
    27661 aatttaataa taagtatcgg gtttgttacg atag
//
LOCUS       DAZKGF010000052        27644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZKGF010000052 DAZKGF010000000
VERSION     DAZKGF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.052"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            230..1816
                     /gene="pdeC"
                     /locus_tag="ACHOQV_002100"
     CDS             230..1816
                     /gene="pdeC"
                     /locus_tag="ACHOQV_002100"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFP9630474.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNSQLQT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGVYLATHVDRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(1819..2142)
                     /gene="soxS"
                     /locus_tag="ACHOQV_002101"
     CDS             complement(1819..2142)
                     /gene="soxS"
                     /locus_tag="ACHOQV_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFP9630475.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            2228..2692
                     /gene="soxR"
                     /locus_tag="ACHOQV_002102"
     CDS             2228..2692
                     /gene="soxR"
                     /locus_tag="ACHOQV_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFP9630476.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLISSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            3238..4587
                     /gene="ghxP"
                     /locus_tag="ACHOQV_002103"
                     /gene_synonym="yjcD"
     CDS             3238..4587
                     /gene="ghxP"
                     /locus_tag="ACHOQV_002103"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFP9630477.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4738..6387
                     /gene="yjcE"
                     /locus_tag="ACHOQV_002104"
     CDS             4738..6387
                     /gene="yjcE"
                     /locus_tag="ACHOQV_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFP9630478.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6470..8119)
                     /gene="actP"
                     /locus_tag="ACHOQV_002105"
     CDS             complement(6470..8119)
                     /gene="actP"
                     /locus_tag="ACHOQV_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFP9630479.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(8116..8430)
                     /gene="yjcH"
                     /locus_tag="ACHOQV_002106"
     CDS             complement(8116..8430)
                     /gene="yjcH"
                     /locus_tag="ACHOQV_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFP9630480.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(8812..10770)
                     /gene="acs"
                     /locus_tag="ACHOQV_002107"
     CDS             complement(8812..10770)
                     /gene="acs"
                     /locus_tag="ACHOQV_002107"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFP9630481.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSIEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            11163..12599
                     /gene="nrfA"
                     /locus_tag="ACHOQV_002108"
     CDS             11163..12599
                     /gene="nrfA"
                     /locus_tag="ACHOQV_002108"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFP9630482.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            12644..13210
                     /gene="nrfB"
                     /locus_tag="ACHOQV_002109"
     CDS             12644..13210
                     /gene="nrfB"
                     /locus_tag="ACHOQV_002109"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFP9630483.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            13207..13878
                     /gene="nrfC"
                     /locus_tag="ACHOQV_002110"
     CDS             13207..13878
                     /gene="nrfC"
                     /locus_tag="ACHOQV_002110"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFP9630484.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            13875..14831
                     /gene="nrfD"
                     /locus_tag="ACHOQV_002111"
     CDS             13875..14831
                     /gene="nrfD"
                     /locus_tag="ACHOQV_002111"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFP9630485.1"
                     /translation="MMQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTKPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPTVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            14851..16569
                     /gene="nrfE"
                     /locus_tag="ACHOQV_002112"
     CDS             14851..16569
                     /gene="nrfE"
                     /locus_tag="ACHOQV_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFP9630486.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            16562..16945
                     /gene="nrfF"
                     /locus_tag="ACHOQV_002113"
     CDS             16562..16945
                     /gene="nrfF"
                     /locus_tag="ACHOQV_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFP9630487.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEAEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            16942..17538
                     /gene="nrfG"
                     /locus_tag="ACHOQV_002114"
     CDS             16942..17538
                     /gene="nrfG"
                     /locus_tag="ACHOQV_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFP9630488.1"
                     /translation="MKQSQIPVKMLTTLTILMAFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            17880..19193
                     /gene="gltP"
                     /locus_tag="ACHOQV_002115"
     CDS             17880..19193
                     /gene="gltP"
                     /locus_tag="ACHOQV_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFP9630489.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(19611..20300)
                     /gene="yjcO"
                     /locus_tag="ACHOQV_002116"
     CDS             complement(19611..20300)
                     /gene="yjcO"
                     /locus_tag="ACHOQV_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFP9630490.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20394..22541)
                     /gene="fdhF"
                     /locus_tag="ACHOQV_002117"
     CDS             complement(20394..22541)
                     /gene="fdhF"
                     /locus_tag="ACHOQV_002117"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22122..22124),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFP9630491.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNKTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDTDQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(22739..24205)
                     /gene="mdtP"
                     /locus_tag="ACHOQV_002118"
     CDS             complement(22739..24205)
                     /gene="mdtP"
                     /locus_tag="ACHOQV_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFP9630492.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPFYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIVAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTLRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQASPVVEKK"
     gene            complement(24202..26253)
                     /gene="mdtO"
                     /locus_tag="ACHOQV_002119"
     CDS             complement(24202..26253)
                     /gene="mdtO"
                     /locus_tag="ACHOQV_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFP9630493.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRKKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYATGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            complement(26253..27284)
                     /gene="mdtN"
                     /locus_tag="ACHOQV_002120"
     CDS             complement(26253..27284)
                     /gene="mdtN"
                     /locus_tag="ACHOQV_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFP9630494.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGIDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(27303..27578)
                     /gene="ytcA"
                     /locus_tag="ACHOQV_002121"
     CDS             complement(27303..27578)
                     /gene="ytcA"
                     /locus_tag="ACHOQV_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFP9630495.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         6168 a         7153 c         7548 g         6775 t
ORIGIN      
        1 gtggtttttc agtggcatta agggcatatc ttcccgccgc ctctgcattc ctgtagaaaa
       61 ttagttttga atatcaatga attattttta tccaggtgac cattaaaaag gtatcaattt
      121 caaatcaggc aaaagtgcta tttataccgt aagatttatc taaagacgtc ggtgcccagg
      181 gttttcacct tgcaatggcc gggtataaac aggcaggaaa ttgatagcaa tgagtcatcg
      241 tgcacgacac caattactgg cgttgccggg cattatcttt ttagttctct ttcccatcat
      301 tctgtcgcta tggattgcct tcctttgggc aaaatcagaa gtgaatagtc agctccaaac
      361 ttttgctcag ctggcgctgg ataaatccga gctggtcatt cgccaggcag atttagtgag
      421 cgatgcagca gaacgctatc aggggcaagt ttgcactcca gcccatcaaa agcgaatgtt
      481 gaatattatt cgtggctatc tttatattaa tgaattgatc tatgcccgtg ataaccattt
      541 tttatgctca tcgctgatag cgtctgtaaa tggctatacg attgcaccgg ccgattataa
      601 gcgtgaacct aacgtttcta tctattatta ccgtgatacg ccttttttct ctggctataa
      661 aatgacctac atgcagcggg gaaattatgt ggcggttatc aaccctctct tctggagtga
      721 agtgatgtct gatgatccga cattgcaatg gggagtgtat gatacggtga cgaaaacctt
      781 tttctcgtta agcaacgagg cctcggcagc aacgttttct ccgctgattc atttgaatga
      841 tttaaccgta caacgaaatg gctatttata tgcgacagtt tattcgacaa aacgcccaat
      901 tgcagccatt gttgcgactt catatcaacg tcttataact catttttata atcatcttat
      961 ttttgcgttg cccgccggta ttttggggag tcttgttctg ctattactct ggctacgtat
     1021 tcgacaaaac tatttgtctc ccaaacgcaa attgcaacgc gccctcgaaa aacatcaact
     1081 ttgcctttat taccagccaa taatcgatat caaaacagaa aaatgtatcg gagctgaagc
     1141 attgttacgt tggcctggtg agcaggggca agtaatgaat ccggcagagt ttattccgct
     1201 ggcagaaaag gaggggatga tcgaacagat aactgattat gttattgata atgtcttccg
     1261 cgatctgggc gtgtacctgg caacacatgt cgatcgctat gtttctatta acctgtcggc
     1321 ctccgatttt catacgtcac ggttgatagc gcgaaccaac cagaaaacag agcaatatgc
     1381 ggtgcgtccg cagcaaatta aatttgaagt gactgaacat gcatttcttg atgtcgacaa
     1441 aatgacgccg attattctgg ctttccgcca ggctggttac gaagtggcaa ttgatgattt
     1501 tggtattggc tactctaact tgcataacct taaatcattg aatgtcgata ttttgaaaat
     1561 cgacaaatcg tttgttgaga cgctgaccac ccataaaacc agtcatttga ttgcggaaca
     1621 tatcatcgag ctggcgcaca gtctggggtt aaaaacgatc gctgaaggcg tcgaaactga
     1681 ggagcaggtt aactggctgc gcaaacgcgg cgtgcgctat tgtcagggat ggttctttgc
     1741 gaaggcgatg ccgccgcagg tgtttatgca atggatggag caattacccg cgcgggagtt
     1801 aacgcgcggg caataaaact acaggcggtg gcgataatcg ctgggagtgc gatcaaactg
     1861 ccgacggaaa acgcgggaga aggtctgctg agagacataa cccaggtcca ttgcgatatc
     1921 aaaaatcgga cgctcggtgg tgcgcaactc aacggcggcc agtaacaggc ggcgttggcg
     1981 aatgtaatca cctagtgtct gatgcgtcac agtacggaac atccgctgta agtaccactt
     2041 tgaatagcct gatttttttg cgacgacatc aatgttaagc ggctggtcaa tatgctcgtc
     2101 aatccatgcg ataagatcct gaataatttt ctgatgggac ataaatctgc ctcttttcag
     2161 tgttcagttc gttaattcat ctgttgggga gtataattcc tcaagttaac ttgaggtaaa
     2221 gcgatttatg gaaaagaaat taccccgcat taaagcgctg ctaacccccg gcgaagtggc
     2281 aaaacgcagc ggtgtggcgg tatcggcgct gcatttctat gaaagcaaag ggttgattag
     2341 cagtatccgt aatagtggca atcagcggcg atataaacgt gatgtgttgc gatatgtcgc
     2401 aatcatcaaa attgctcagc gtattggcat tccgctggcg accattcgtg aagcatttgg
     2461 cgtgttgccc gaagggcata cattaagtgc gaaagagtgg aaacagcttt cgtcccaatg
     2521 gcgagaagag ttggatcggc gcattcatac cttagtggcg ctgcgtgacg agctggacgg
     2581 atgtattggt tgtggctgcc tttcgcgcag tgattgccca ttgcgtaacc cgggcgaccg
     2641 cttaggagaa gaaggcacag gcgcacgctt gctggaagat gaacaaaact aaagcgccac
     2701 aagggcgctt tagtttgttt tccggtcttt gtctttctct ctatcccgct ggtacacagg
     2761 agggtttccc ccgacgtcaa cacacctcat tcgagcacgt ggtggaggtt ccggttggtg
     2821 ttgatgcttt aattgtatgt caccggcgtt tcttcgccag tgtaaaagta cactttttag
     2881 ccgcaatatt tttgtcatct cagacgattt tttatcgcaa tcctgaacgg tatacggctc
     2941 gataacgctg caatcttgcg catcgacgat aacgtttgcg catcaattgc ctggtttttc
     3001 atcgtcaaga caataaaaga gaaaaaagca gcaaacttcg gttgaaaaag ccgctatgat
     3061 cgccggataa tcgtttgctt tttttaccac ccgttttgta tgcgcggagc taaacgtttg
     3121 cttttttgcg acgcaccaat ttgtcgcaaa cctggagcag gaagataacg tttcgctggc
     3181 aggggattgt ccgccacgca tcttgacgaa aattaaactc tcaggggatg ttttcttatg
     3241 tctacgccat cagcgcgtac cggcggttca ctcgacgcct ggtttaaaat ttcacaacgt
     3301 ggaagcactg tccgtcagga agtggttgcc gggttaacaa catttctggc gatggtctac
     3361 tcggttatcg tcgttccagg catgttgggt aaagcaggct tcccgcctgc ggcagttttc
     3421 gttgcgacct gtctggttgc cggactcggt tctatcgtga tgggtctgtg ggctaatctg
     3481 ccgttggcga ttggttgcgc catctccctg acggcgttta ccgcattcag tctggtgctg
     3541 gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt tcctgatggg tgtgctgttt
     3601 acggtcattt ctgccacggg tatccgtagc tggattttgc gcaacttgcc gcacggtgtg
     3661 gcgcacggca cggggattgg tatcggtctg ttcctgctgc tgattgctgc taatggtgtc
     3721 ggtctggtga ttaaaaaccc acttgatggt ctgcccgtcg cgctggggga tttcacgacc
     3781 tttccggtga tgatgtcgtt ggtggggctg gcagtgatta tcggcctgga aaaactgaaa
     3841 gtccctggtg gcattctgct gaccattatc ggtatctcaa ttgtcggttt gatcttcgat
     3901 cctaacgtcc atttctccgg cgttttcgcc atgccttcat tgagcgatga aaacggcaat
     3961 tcactaattg gcagcctgga cattatgggc gcgctgaatc ctgtagtcct gccaagcgtt
     4021 ctggcgctgg tgatgacggc agtatttgat gccaccggaa ctatccgtgc cgtcgccggc
     4081 caggcgaacc tgctggataa agatgggcag atcatcgacg gcgggaaagc actgaccact
     4141 gactccatga gcagcgtttt ctctggcctg gtgggtgcag ctccggcagc ggtatacatc
     4201 gagtctgcgg cgggtacggc ggcgggcggt aaaaccggtt tgacggctat caccgttggc
     4261 gtgctgttcc tgctgattct gttcctctct ccgctctctt acctcgttcc ggggtatgca
     4321 acggctccgg cgctgatgta cgttggcctg ctgatgctga gtaacgtggc gaaaatcgac
     4381 tttgctgatt ttgttgatgc gatggcgggt ctggttacgg cggtattcat cgtgctgacc
     4441 tgtaacatcg taacaggcat catgatcggc ttcgcgactc tggtgattgg tcgtctggtt
     4501 tccggcgaat ggcgcaagtt gaacatcggt acggtcgtta tcgctgtggc gctggtgacc
     4561 ttctatgcgg gtggctgggc tatctaatct ttcatatgat tctgaaaacg ggtggcaatg
     4621 gctgcccgtt tttattttct ccgcacattg tgtgagcttt tgcgatattc tgaaaaaatg
     4681 agaattcagg cataacgcct ttctcagatc ataaaaaaca tcgcaaacag ggaacgcatg
     4741 gaaatcttct tcaccatact gataatgacc ctcgtggtct cgctgtccgg ggtggtcact
     4801 cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa tcgccatcgg tgcgctactg
     4861 gcgtggccga cgtttggttt gcatgtggag tttgatcctg aactcttttt agtcttgttt
     4921 atcccgccgt tgctgttcgc tgatggctgg aaaacgccga cccgtgaatt tctcgaacat
     4981 ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg tcaccgtggt cggcattggc
     5041 tttcttattt actgggtggt gccgggcatt ccgctgatcc ccgcctttgc gctggcggcg
     5101 gtgctttctc cgaccgatgc tgtggcgctc tcggggattg ttggcgaagg gcgcatcccg
     5161 aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga atgacgcctc cggtctggtg
     5221 tcgttgaagt ttgccgtggc agtggcgatg gggacgatga tcttcaccgt cggtggtgcg
     5281 acggtcgaat ttatgaaagt ggccattggc ggtattctcg ccggttttgt ggtgagctgg
     5341 ctgtacggtc gctcgctgcg attcctcagc cgctggggcg gtgatgaacc cgcgacgcag
     5401 atcgtcctgc tgttcctgct gccattcgct tcttatctga ttgccgaaca tattggcgtt
     5461 tccggtatcc tcgctgcggt tgccgccggg atgaccatca cccgttccgg tgtgatgcgc
     5521 cgtgcgccgc tggcaatgcg cctgcgcgca aacagtacct gggcgatgct ggaatttgtc
     5581 tttaacggca tggtattcct gctgttaggt ctgcagctgc cgggtattct ggagacgtcg
     5641 ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga tgctgtttac cgatatcatt
     5701 ctgatttatg cggcgctgat gctggtccgt ttcggctggc tgtggacgat gaaaaagttc
     5761 agcaaccgct tcctgaagaa gaagccgatg gagtttggtt cgtggaccac acgagaaatc
     5821 ctgatcgcgt ctttcgccgg ggtgcgtggg gcgatcactc tggctggtgt gctctcaatc
     5881 ccgctgctct tgccggatgg taacgtcttc ccggcgcgct atgagctggt gttcctggcg
     5941 gctggtgtca ttctcttctc gctgtttgtc ggcgtggtga tgttgcctat tctgctacaa
     6001 cacattgaag tcgcggacca ttcgcaacaa ttgaaagagg aacgtattgc gcgagcggca
     6061 acggcagaag tggcgattgt ggcgatccag aaaatggagg agcgtctggc ggcggatacc
     6121 gaagagaata tcgataacca gctgctcaca gaggttagtt ctcgcgtcat tggtaacctg
     6181 cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg tgcaggaaga gaaccttgag
     6241 cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg aactgtacca cctgcgcgcc
     6301 acgcgggaga tcagtaacga aacgctgcaa aaattactgc acgatctcga tttgcttgaa
     6361 gcgttgctga ttgaagagaa ccaataacgc tttggcggtg taatcggtaa aaaggtgcag
     6421 tacatcgcac ctttttaccg ttggtcaggt gaccagaggc tagtggagat taatgcgcgc
     6481 ggccttgctc aacgccaaag ccagtctggg agcggataaa ctgcgcacgg aacagttcac
     6541 gctcgcgcgc accttccgct gaattatccg ttgccgagaa gaaccagata ccgaggaatg
     6601 ccacgctgat agagaacagt gccgggtatt catacgggaa gatggctttt tcgtgaccga
     6661 ggatctgtac ccaaatcgtc ggaccgagga tcatcagcac caccgcagta atcagtccta
     6721 accagccgcc cagcattgcg ccacgggtag tcagtttcga ccagtacatg gaaagcagaa
     6781 tgatcgggaa gttacagctc gccgcgatgg caaacgccag ccccaccata aaggcgatgt
     6841 tctgattctc aaacagcacg ccgaggataa tggcaatcac acccaaaatc agaacggtga
     6901 ttttcgatac ccgcagctct tcacgttcgg tcgcgccttt tttgaagacg ttagcgtaca
     6961 ggtcgtgaga caccgccgat gcgcccgcca gcgtcagccc cgctaccacc gcgaggatag
     7021 tggcgaaagc aaccgctgaa ataaagccga ggaacaggtt gccgcccacc gcattggcca
     7081 ggtgaactgc cgccatgttg ttaccaccaa tcagatggcc cgcagcatct ttatattccg
     7141 gattcgcacc aaccagcatg attgcgccga agccgataat aaaggtcagg atgtagaagt
     7201 agcccataaa tccggtggcg tagaacacgc tcttacgtgc ttcgcgggca tcgctgaccg
     7261 taaagaagcg catcagaata tgcggcaagc ccgccgtacc aaacatcagt ccgagcccca
     7321 gcgacagtgc tgagatcgga tctttcacca gcccgcctgg cttcatgata tcgacacctt
     7381 tcgggtgtac cgccatcgct tcgctgaaca gattgttgaa gctaaagccg acgtgtttca
     7441 tcaccataaa ggccataaag ctggcaccga acagcaacag aacggctttg ataatctgta
     7501 cccaggtggt cgccagcatg ccgccgaaca ggacgtacat catcatcagc acgccgacca
     7561 gcaccaccgc aatgtgatag ttaaggccaa acagcagctc gatgagttta cctgcgccca
     7621 ccatctgggc gataaggtaa agcgccacca ccaccagaga accacaggcc gaaagaatac
     7681 gaatcggccc ttgtttcagg cggtaagagg ctacatcggc aaaggtgtag cggcccaggt
     7741 tacgcagacg ttcggcaatc aggaacagaa tgattggcca gcctaccagg aagcccagcg
     7801 agtaaattaa gccgtcatag ccggaggtaa acaccagcgc ggagatcccc aagaatgagg
     7861 cggcggacat atagtccccg gcaatcgcca gcccattctg aaaaccggtg atattgccgc
     7921 ctgcggtgta gtagtcatta cgcgaacgta cgcgttttga cgcccagtag gtaatgccga
     7981 gcgtaaacac gacgaaaatc aggaacataa taatcgcctg ccagtttgtt ggctggcgct
     8041 ctacggcccc gctaatagca tccgcggcgt tagctgcgaa agggagtgtg gcggcaagcg
     8101 ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg acttcattat taagacggtc
     8161 gaattcgccg ttcgcccgcc agatgtagat accagtgaga acaaaggaga tcacaattac
     8221 tccaacacca atcggaatac cgcgtgtgac gctggtgttc ggattcagcg gggtgcccag
     8281 ccagccgggc gcgaaggcga tcagtaaaat aaagccgata taaactgcca gcataataat
     8341 cgacaggatg gtggcaaacc gttgccgttt ttcgactaac tccctgaaat gcgcattgtc
     8401 ttctatccgc tgataaatcg taccattcat cacagattct ccagaggtaa tgtagggatt
     8461 gttttaattc ccgctccctt atgggagaag gttaacgctt gggtaaccct tgccgaatgt
     8521 aggccggata aggcattcac gccgcatccg gcaatcaacg cctgatgcga cgctgtcgcg
     8581 tcttatcagg cctacagccg ttgccgaatg taggccggat aaggcgttta cgccgcatcc
     8641 ggcaatcaac gcctgatgcg tcgctgtcgc gtcttatcag gcctacagcc gttgccgaat
     8701 gtaggccgga taaggcgttc acgccgcatc cggcaaccag cgcctgatgc gacgctgtcg
     8761 cgtcttatca ggcctacaaa ccgttaccga ctcgcatcgg gcaattgtgg gttacgatgg
     8821 catcgcgata gcctgcttct cttcaagcag cttctcgact acgccaggat cggcaagcgt
     8881 cgaggtatcg cccaggttgc tggtatcgcc cgccgcaatt ttgcgcagaa tacggcgcat
     8941 aattttgccg gagcgggttt taggcaggga gtcggtccag tgcagcacgt ctggcgtcgc
     9001 cagcgggcca atctctttac gcacccagtt gcggacttct gcgtacagtt ctggtgacgg
     9061 ttcctcccca tgattaagcg tgacgtaggc gtagatcgcc tgacctttaa tattgtgcgg
     9121 aataccgacg acagcggctt cggcgatttt cggatgcgcc accagcgccg actcaatctc
     9181 cgccgttccc agacggtgac ccgagacgtt cagcacgtcg tccacgcgcc cggtgatcca
     9241 gtaatagcca tcttcatcgc gacgcgcgcc gtcgccgctg aaatacatat ttttgaaggt
     9301 ggagaagtag gtctgctcaa aacgttcgtg atcgccaaac agcgtacgcg cctgacccgg
     9361 ccaggagtcg gtgatcacca gactgccttc ggtggcccct tccagcgggt taccttcgtt
     9421 atcgaccagc gccggttgca cgccgaagaa cggacgtgtt gccgaaccgg ctttcagctc
     9481 ggtagcgcca ggcagcgggg tgatcatgaa accgccggtt tcggtctgcc accaggtatc
     9541 gaccaccggg catttctcgt tgccgatttt tttccagtac cactcccacg cttccgggtt
     9601 gattggctcg cctacggaac cgagaatgcg cagcgacgaa cggtcagtgc cttcgatcgc
     9661 tttatcgcct tccgccatca gcgcgcggat cgccgtgggc gcggtataga gaatattgac
     9721 ctgatgcttg tccaccacct gcgccatacg ggcaggcgtc ggccagttcg gtacgccttc
     9781 aaacatcagc gtggtcgcgc cgcaggccag cgggccgtac agcaaataac tgtgtccggt
     9841 aacccagccc acatcggcgg tgcaccagta gatatcaccc ggatgataat caaagacata
     9901 tttaaaggtc agcgccgcgt acaccagata accgccggta gtgtgcagca cgcctttcgg
     9961 cttcccggta gaaccggagg tgtagagaat aaacagcgga tcttcggcgt tcatctcttc
    10021 cgcctggtgc tgatcgctgg cttgctcaac caggtcgtgc caccacaggt cgcgcccttc
    10081 ctgccagtca attttcccgc cagtacgctt cagtaccacc acatgttcaa tgctggtgac
    10141 gttcgggttt ttcagcgcgt catcaacgtt tttcttcagc ggaatactac gcccggcacg
    10201 cacgccttcg tcggaagtga tcaccagtcg tgagttagaa tcgataatgc gtccggcaac
    10261 ggcttccggc gagaagccgc cgaaaattac cgaatgcacc gcgccaatgc gggcgcaggc
    10321 cagcatcgcg accgcggctt ccggcaccat cggcatataa atcgccacca catcaccttt
    10381 tttaatgccc agctcgagca gggtattggc gaagcggcag acgtcgcggt gcagctcttt
    10441 atagctgata tgtttgctct ggctggcgtc gtcgccttcc cagatgatgg cggtacgatc
    10501 gccgttttct tgcagatggc ggtcaaggca gtttgccgcc agattcagag tgccgtcctc
    10561 gtaccattta atggacacat taccgggggc aaaggaggtg tttttcacct tctggtaagg
    10621 tttgatccaa tcgagaattt ttccctgttc gccccagaag gtatcaggtg cgttaataga
    10681 ttgttgatac atcgcctcgt actgctgagg gtttatcagg caacggtctg cgatgttggc
    10741 aggaatggtg tgtttgtgaa tttgactcat gcttttgttc tccttgtagg atgttaataa
    10801 tatgtggcat aagcgttaaa tgtaggggta ttggcagttt tgtttagtat ttgggcgaca
    10861 gatcacgcaa aagtagaatt gtgcaaataa acggcagggt aatttttgaa ggtcagcaac
    10921 aaaagttgat taattctttg aggaacatgc agttatgcat gctgttgaaa aagaggaaga
    10981 tactgactaa ctctaaagtg gtattttaca tgcacttaca attgattaaa gacaacattc
    11041 acagtgtggt tatttgttac acataggggc gagcaatgtc atgacagtgt aggtgcggtt
    11101 actgtcgtga aaaacaataa aaaccgccat tgcaacaatg gcgcaattcg gatgaagccc
    11161 ctatgacaag gataaaaata aacgcacgcc gtatcttcag cttattgatt ccttttttct
    11221 ttttcacttc tgttcacgct gaacaaacgg cagctcctgc aaaacctgta actgttgaag
    11281 cgaagaatga aacctttgcc ccgcaacatc ccgatcaata tctctcctgg aaagccacct
    11341 cggaacagtc agagcgtgtt gacgccctgg cggaagatcc acggctggtg atcctgtggg
    11401 cggggtatcc cttctcgcgc gattacaaca agccgcgtgg acatgccttt gctgtgaccg
    11461 atgtgcgtga aaccctgcgt accggtgcgc cgaaaaacgc tgaagatggt ccgctaccga
    11521 tggcatgctg gagttgtaaa agcccagatg tggcgcgtct gatccagaaa gacggcgaag
    11581 atggctactt ccacggaaaa tgggcgcgcg gcggcccgga aatcgtcaac aacttaggtt
    11641 gtgccgattg ccataacacc gcctcaccag agttcgccaa aggcaaaccg gagttaaccc
    11701 tttcccgtcc gtatgcggct cgcgcgatgg aagccattgg taaacctttt gagaaagccg
    11761 gacgtttcga ccagcaatcg atggtttgcg gtcagtgcca tgtggagtat tacttcgacg
    11821 gcaaaaacaa agcggttaaa ttcccgtggg atgacggcat gaaagtcgaa aatatggagc
    11881 agtattacga caaaattgcc ttctctgact ggactaactc cctgtcgaaa acgccaatgc
    11941 tgaaagcgca gcacccggaa tatgaaacct ggacagcggg cattcacggc aaaaacaacg
    12001 tgacctgtat cgattgccat atgccaaaag tgcagaacgc cgaaggcaaa ctctacaccg
    12061 accataaaat tggtaatccg tttgataact tcgcccagac ttgtgcgaac tgccataccc
    12121 aggacaaagc tgccttgcaa aaagtggtcg cggaacgtaa gcagtcgatt aacgacctga
    12181 aaatcaaggt tgaagatcaa ctggttcacg ctcacttcga agcgaaagcg gcgctggatg
    12241 caggcgcaac ggaagccgaa atgaagccga ttcaggacga tatccgtcat gcccagtggc
    12301 gttgggattt ggcgatcgct tcccacggca ttcatatgca cgcaccggaa gaaggtctga
    12361 ggatgctcgg tacggcgatg gataaagcgg cggatgcacg caccaaactg gcgcgcctgc
    12421 tggcgaccaa aggcattacc catgaaatcc agatcccgga tatctcgacc aaagagaaag
    12481 cccagcaggc cattggcctg aacatggagc aaatcaaggc cgagaagcag gacttcatca
    12541 aaacggtgat cccgcagtgg gaagagcagg cacgtaaaaa cggtctgtta agccaataac
    12601 cccgttccgc ctcgcaaggg gtggaaaaca caatggagtg aatatgagcg tattacgttc
    12661 gttattaact gccggggtgc tggcgtcggg cctgttgtgg agcctgaacg gaattaccgc
    12721 tacccctgcg gcgcaggcat ctggtgatcg ctacgaagtt acccagcagc gtaacccgga
    12781 tgccgcctgt ctggactgtc ataagccaga taccgaaggt atgcatggca aacatgcttc
    12841 cgttatcaac ccgaataaca aactgccggt cacctgcacc aactgccacg gtcagccatc
    12901 accgcaacac cgcgaagggg tgaaagatgt gatgcgcttt aacgagccga tgtacaaggt
    12961 tggagagcag aacagcgtct gtatgtcctg ccatctgccg gaacagttgc aaaaagcgtt
    13021 ctggccacac gatgtccacg taaccaaagt ggcgtgcgcc agctgccatt ccctgcatcc
    13081 acagcaagat acgatgcaga cgttaagtga caaaggacgg attaagattt gcgtcgattg
    13141 ccacagcgat cagcgcacca atccgaactt taacccggcg tctgttccgt tgcttaagga
    13201 gcagccatga cctggtctcg tcgccagttt ctcaccggcg tcggcgtgct ggcagccgtc
    13261 agcggcaccg cagggcgcgt ggtggcgaaa acgttgaata tcaatggggt gcgttacggc
    13321 atggtgcatg acgagtcgtt atgcatcggc tgtaccgcct gtatggatgc ttgtcgggaa
    13381 gtgaacaagg tgccggaagg cgtctcgcgc ctgacgatta ttcgtagcga gccgcagggc
    13441 gaatttcctg atgtgaaata tcgcttcttc cgtaagtctt gccagcactg cgatcatgcg
    13501 ccgtgcgttg acgtctgccc gaccggtgcg tcttttcgtg atgccgctag cgggattgtc
    13561 gatgttaatc ctgatctctg cgtcggttgt cagtactgca tcgccgcctg tccgtaccgc
    13621 gtgcgcttta tccatccggt cacgaagacg gcggacaaat gcgatttctg ccgtaagacc
    13681 aatttgcagg ccggtaagct gcccgcgtgc gttgaagctt gcccgaccaa ggcactgacg
    13741 tttggcaatc tggacgatcc caacagtgag atttcgcaac tgctgcgcca gaagcccact
    13801 taccgctaca agctggcgct gggaaccaaa ccgaagctgt accgcgtacc gtttaaatac
    13861 ggggaggtga gccaatgatg cagacttccg catttcattt tgaatcgctg gtgtgggact
    13921 ggccgattgc catctacctg tttttgattg gtatttctgc cggtctggtg acgctggccg
    13981 tgctgttacg tcgcttctac ccgcaggcgg gcggtgcaga cagtacgttg ctgcgcacca
    14041 cgctaattgt cgggcctggc gcggtgatcc tcggtctgtt gatcctcgtc ttccacctga
    14101 caaagccgtg gaccttctgg aagctgatgt tccactacag ttttacctcg gtgatgtcga
    14161 tgggggtgat gttgtttcag ctctacatgg tggtgctggt gctgtggctg gcgaaaatct
    14221 ttgaacatga tttgcttgcc ctgcaacaac gctggttgcc gaagctgggg atcgtgcaaa
    14281 aggttctgag cctgctgacg cccgttcatc gcggactgga aacattgatg ctggtgctgg
    14341 cggtgctgct gggggcttat accggctttc tgctgtcggc gctgaaatcg tatccgttcc
    14401 tcaataaccc gatcctgccg gtgctgttcc tcttctccgg catctcgtcc ggtgcggcgg
    14461 tagcgctgat cgccatggcg atacgccaac gcaaaaatcc gcattccacg gaagcgcact
    14521 ttgttcaccg tatggaaatc cctgtggtat ggggtgaaat cttcctgctg gtggcgtttt
    14581 ttgtcggtct ggcgctgggc gatgacggta aagtgcgtgc gctggtggcg gcattaggtg
    14641 gcggtttctg gacgtggtgg ttctggcttg gtgtcgccgg gctggggctg attgtgccaa
    14701 tgttgctcaa accgtgggtc aatcgcagtt ccggcattcc taccgtgctg gcggcgtgtg
    14761 gggccagtct ggtcggcgtg ttgatgctgc gctttttcat tctctacgcc ggacagttga
    14821 cggtggcgta agccagaaaa gaggtggttt ctggacgtat tccttcctga agtcggtttt
    14881 ctggcgttgt tgttaagtct cggggtcaac gtgttgaccc cgttgacggc cttcgcggga
    14941 gtgcggttgc gctggcctgc catgatgcga ctcacttgca tcggcattct ggcgcagttc
    15001 gcgctcctgc tgctcgcctt tggcgtactg acgtattgtt ttctcatcag cgatttctcg
    15061 gtcatttatg tcgcccaaca tagctacagc ctgctgtcgt gggaactcaa actggcggcg
    15121 gtgtggggcg gtcatgaagg ttcgctgctg ctttgggtgc tgctgctttc cgcctggagc
    15181 gcgctgtttg cctggcatta tcggcagcaa accgatccgc tatttccgct aacgctagcc
    15241 gttttatctc tcatgctcgc cgcactgcta ctgtttgtag tgctgtggtc cgatcccttc
    15301 gtgcggatat ttccaccagc aatcgaaggc cgcgatctca atccgatgct gcaacatccc
    15361 ggtcttatct ttcatccacc gctgctttac cttggctatg gcggtttgat ggtggcggcg
    15421 agcgtggcgc tggcgagttt actgcgcggc gagtttgatg gtgcctgcgc ccgaatttgc
    15481 tggcgctggg cgttacctgg ctggagtgca ttaacggctg ggatcatcct cggttcctgg
    15541 tgggcctatt gcgaactggg ctggggcggc tggtggttct gggatccggt ggaaaacgcc
    15601 tctttattac cctggctttc tgccactgcg ctgctgcaca gtttgtccct gacacgccag
    15661 cgggggattt tccgccactg gtcgctgctg ctggcgatag ttactctgat gctgtcgctg
    15721 ctgggcacct taattgtccg ttctggcatt ctggtttcgg ttcatgcgtt cgcactggat
    15781 aacgtccgcg ccgtgccgtt gttcagcctg tttgcactga ttagccttgc gtctctggct
    15841 ctgtatggct ggcgagcgcg ggacggtggc ccggcggtgc gtttttcggg gttatcgcgg
    15901 gaaatgttaa tcctcgctac gctgttgctg ttttgcgcag tgctactgat cgtgctggtg
    15961 ggaacgcttt atccgatgat ttacggtctg ctgggctggg gacgcctctc cgttggcgcg
    16021 ccgtatttta accgcgcgac gttaccgttt ggtctgttga tgctggtggt gattgtgctg
    16081 gcgacgtttg tctctggcaa acgcgtgcag cttccggcgc tggtagctca tgctggcgtg
    16141 ctgttatttg ccgcggggat cgtggtttcc agcgtcagtc gtcaggagat cagcctgaat
    16201 ttacagccgg gtcagcaggt gacgctggca ggatacacct tccgttttga gcgactcgat
    16261 ctgcaagcca gaggcaatta caccagcgaa aaagcgatag tggcgctgtt tgaccatcag
    16321 caacgcattg gtgaactgac gccggagcgg cgtttttatg aagcacgccg tcagcaaatg
    16381 atggaaccgt caattcgctg gaacggcatc catgactggt atgcggtcat gggcgagaaa
    16441 accggagcgg atcgttacgc ttttcgtttg tatgtacaaa gcggtgtgcg ctggatctgg
    16501 gggggaggat tgttgatgat tgcgggcgca ttgttaagcg gatggcgggg gaggaagcgc
    16561 gatgaataaa ggacttctca cgctactgct gttatttacc tgttttgcgc gcgctcaggt
    16621 cgtagacacc tggcaattcg ccaatccgca acaacagcaa caggcgttaa atattgccag
    16681 ccagttacgt tgtccgcagt gccagaatca aaacttactg gaatccaacg cgccggtggc
    16741 ggtcagtatg cgccatcagg tttacagcat ggtggcggag gggaagaacg aagctgaaat
    16801 catcggctgg atgaccgaac gctacggaga ttttgttcgc tataacccac cgttaacggg
    16861 ccagacgctg gtgttgtggg cgctgccagt ggtgttgtta ctgctgatgg cactgatcct
    16921 ctggcgagtg agggcgaagc gatgaaacag tcacaaatac cggtgaaaat gctgacaaca
    16981 ctcacgattt tgatggcatt tctctgtatc ggcagttatc tgttaagccc aaaatggcag
    17041 gcggtacgtg cggagtatca gcgtcagcgc gatccgctgc atcagtttgc cagccagcaa
    17101 accccagaag cgcagcttca ggcattgcag gataaaatcc gcgctaatcc acaaaacagc
    17161 gaacagtggg cgttactggg cgagtactat ctgtggcaaa atgattacag caattcgctg
    17221 ctggcgtacc gtcaggcgtt gcaactacgt ggtgagaacg ctgaactgta tgcggcgctg
    17281 gcgacggtgc tttattacca ggccagccag catatgaccg cccagacacg cgcaatgatc
    17341 gataaagccc tcgcgctgga ctctaacgaa atcaccgctc tgatgctgct ggcttccgat
    17401 gcgtttatgc aggcgaatta cgcgcaggcc atcgaactat ggcaaaaagt gatggatctc
    17461 aactcacccc ggatcaaccg gacacagctg gtcgagtcga ttaatatggc gaaattgttg
    17521 cagcggagat cagattaatc atctggtgat gaagattatg ttattgagct gcataaaaaa
    17581 taatcgaatg aacatatgcc aaaaataatc accaatcagt attattgcag attaaacaaa
    17641 taaaaatctt tccataacaa atggttattc attaatcctg ccataaactc gcgttattat
    17701 gcattaatgc agcgaaaagc tctgttgtta aagggttgcg caacataccg cgcaaatgat
    17761 actgatcata agcgttaaaa aaatctacaa accaacgcaa cacaattcat gccctggcag
    17821 tatgtcacgt tctcgcgttt ctgaacgggg aacggcgctc cattgaggaa gttattctga
    17881 tgaaaaatat aaaattcagc ctggcctggc agattctgtt tgctatggtg ctgggcattc
    17941 tcctgggaag ctacctgcac taccatagcg acagccgcga ctggctggtc gtcaatttgc
    18001 tctctccggc gggtgatatc ttcatccatc tgatcaaaat gattgttgtg ccgattgtga
    18061 tctccacgct ggtggtgggt atcgcgggtg ttggtgatgc caaacagctc gggcgtattg
    18121 gcgcgaaaac cattatttac ttcgaggtga tcaccaccgt cgccatcatt ttggggatca
    18181 ctctggcgaa cgtcttccag cccggtgccg gggtggatat gtcgcagctg gcgaccgtcg
    18241 atatctcgaa atatcagagc actacggaag cggtacaaag cagttcccac ggcattatgg
    18301 gcacgatttt gtcgctggtg ccgacgaaca ttgtggcgtc gatggcgaaa ggcgaaatgc
    18361 tgccgatcat cttcttctcg gtgctgtttg gtctggggct ttcatccctg cccgcgacgc
    18421 atcgtgaacc gctggtgacc gttttccgct ccatctctga aaccatgttt aaagtgactc
    18481 acatggtgat gcgttatgcg ccggtgggtg tgtttgcgct gattgcggtg acggtggcta
    18541 actttggttt ctcgtctctg tggccactgg cgaaactggt gctgctggtg catttcgcca
    18601 ttctgttctt cgcgctggta gtgctgggaa ttgtggcgcg cctgtgcggg ttaagcgtct
    18661 ggatcctgat tcgtattctg aaagatgagc tgattctggc gtactccact gccagctctg
    18721 aaagcgtgct gccgcgaatt attgagaaga tggaagccta cggagcaccg gcgtcgatca
    18781 ccagtttcgt ggtgccgacc ggttactctt ttaaccttga tggttcgacg ctgtatcaaa
    18841 gtattgccgc tattttcatc gcgcagttgt atggcattga cctgtccatc tggcaggaaa
    18901 tcattctggt gctgacgctg atggtgacct cgaaagggat tgctggcgtg ccgggcgtgt
    18961 cgtttgtggt gttgctggca acgctgggta gcgtaggtat tccgctggaa ggtctggcgt
    19021 ttattgccgg tgttgaccgt atcctcgaca tggcgcgtac tgcgctgaac gtggtgggta
    19081 atgcgctggc ggtgctggtg attgccaagt gggaacacaa atttgaccgt aagaaagcgc
    19141 tggcttatga gcgcgaagtg ctgggcaaat ttgataaaac tgcggatcaa taattgaaga
    19201 ttgccgggga tatccacccg gcaatatgtg gatgcctgat gcgacgctta ccgcgtctta
    19261 tcaggcctac gccagactgc gcaatagctt gatttagggt gattttgtag gtcggataag
    19321 gcgtttacgc cgcatccgac atcaatatct gatgcgacgc ttgccgcgtc ttatcaggcc
    19381 tacgccagac tgcgcaatag cttgatttag ggtgattttg taggtcggat aaggcgttta
    19441 cgccgcatcc gacatcaata tctgatgcga cgcttaccgc gtcttatcag gcctacgcca
    19501 gactgcgcaa tagcctgatt tagcgtgatt ttgtaggtcg gataaggcgt ttacgccgca
    19561 tccgacatca atgcctgatg cgacgcttac cgcgtcttat caggcctacc ttaaccgtta
    19621 gttaattttt caaactcttc acacccggta tcaaaccctt ccatacagct cagatttaac
    19681 cagtgcagcg ccttttgttt gttcttctcg ataaaacctt cttcaccgtt taagaacatc
    19741 attcccgccc agtactcgga ataaccggtt cgggaaatcg cagagctgcg tttgaaatac
    19801 caggttgcct tgtcatcgtc ggccttaatg cccacgccgt tggcgtaaat caaaccaagc
    19861 agcatttggg catcgacggc agagtcgttc tccagatctt ccgaggcgtt ttctaacagc
    19921 gaaattgcct ttggataatc cggtttaccc gcttgcgtat ttaccagaat atgcgccagc
    19981 gttacttcac cttctttact gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt
    20041 ggatagtcca gactgaccgg attggtgatt ttgatctgcg ctaacagcgc gcaggcatca
    20101 gcatcaccgc tgtcggcggc tttctgtgcc caatattcgg ctttgctcaa atcgccggag
    20161 ctaaaccagc tgtcggcaag aaaatattgt gcgcgtcggt cccctgcctc tgctgccttt
    20221 aaatactggc tgccaggctc ggagtcgttc gcgtgggcaa agaatgtcaa aaacaacatc
    20281 aatgcgataa tttttttcat tttgataata cgattgggta gggagtaacc agtataaaga
    20341 gttcgaatgg ataaaaaaac agcctccgaa aggaggctgt agaaaggact gtattacgcc
    20401 agtgccgctt cgcgcaggcg agttttcaac ttgttgtact cgtcaatcac gtactgctcg
    20461 gcggcgcgct gatcggcgat cggctcgacg cgcacggcgc agtatttgta ttccggcgtt
    20521 ttcgtaatcg ggcttaagtt ttcggtaacc agctcgttac aggcaccaat ccaccactgg
    20581 taggtcatgt aaatcgcccc tttgttcgga cgatcgctga cctgcgcacg ggtgataatt
    20641 ttgcctttac gcgagtgcac ccaaaccaat gcctcatctt caatacccag acgtttggcg
    20701 tcttcggtat tgatttgtgc gtagccaggt tcatcagcca gcgccgccag tgccgcacag
    20761 ttaccggtca tcgaacggca agagtagtga ccaacttcac gcaccgttga cagtaccatc
    20821 gggtactcgt cggtgagttt gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc
    20881 agaccgttcg gggtatcaaa cttctcttta aacagataag aagtcccctg atcggtatct
    20941 gaagtatcgc ggcaaggcca ctgaatgaag cccagttcgc ccattttttc gtaagtcgca
    21001 ccgtagaaat ccgggcacag atgacgcaac tcatcccaga tctcctgggt tttgttgtag
    21061 tgcatcggat aacccatacg ggtggcgatt tcactgataa tctgccagtc cgttttcaga
    21121 tcccacttcg gctcaaccgc cttgaagaaa cgctggaagc cacggtcagc cgcagtaaac
    21181 acgccttcat gctcgcccca cgacgttgac ggtaaaatga catccgccgc cgacgcggtt
    21241 ttggtcataa agatgtcctg aacgataacc agttccagat cttcaaaggc tttacgtact
    21301 gccgacagtt ccgcgtcagt ttgcagcgga tcttcgccca taatgtacgc ggcacgcact
    21361 tcaccatgcg ctgcgcggtg cggcagctcg ctgatgcgat aaccggtatg agcaggcagg
    21421 ctttccacgc cccaggcttt ggcgaatttc tcgcggttag ccggatcttt cacgtactgg
    21481 tatcccggat acgtatccgg cagcgcgccc atatcgcagg caccctgcac gttgttctga
    21541 ccacgaaccg ggtttacacc cgcatgaggc ttaccgaggt taccagtcag catcgcgagg
    21601 ctggtcagag aacgcacggt ttccacgccc tggtagaact gggttacacc catgccccac
    21661 aggatggcgg cgcttttcgc ctgggcatac atccgcgccg cctgacgaat ctcactggcg
    21721 ctgacgccgg tgatatcttc aaccgactcc ggcgtgtagc cttcaacgat tttacgatac
    21781 tcttcaaagc cttctgtacg ggaagcgacg aaagctttgt cgtacaggtt ttcttcaata
    21841 atgacatggc ccatcgcatt caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc
    21901 atgtcagcaa tgcgcgcggt ttcaattttg cgcggatcgc agacgataat tttcgccccg
    21961 ttacgtttag cgttaattac gtggttcgcc acgattgggt gggaatccgc cgggttgtac
    22021 ccgaaaacga acactaaatc ggtattatca atttcgttaa tagcattgct cattgcgcca
    22081 ttaccgaccg attggtgcag acctgcaacc gatgggccgt gtcagacacg agcgcagcag
    22141 tcaacgttat tggtaccaat aacggcgcgc gcaaattttt gcattacata gttggtttcg
    22201 ttacccgtac cacgcgagga gccggtcgtc tggatggcat ccggaccgta cttctctttg
    22261 atggcgctca ggcgctcggc aacgtaattc agtgcctcat cccaggaaac aggttcgagt
    22321 ttgccgccac gctggcgacg gatcatgggg gttttcaggc gcggggtcag gatctgggta
    22381 tcgttaatga aatcccagcc ataataaccc ttcagacaca gggtaccctg gttggttttc
    22441 ccctgcgctg cctccgcccg gacgattttg ccgttatcga cgaccaggtt gattttgcaa
    22501 cctgatgcgc aataggggca aaccgtgacg acttttttca tcggtctcgc tccagttaat
    22561 caaatcacgc atacgcgctc tcgactacag tatgcatctt ttatgccaca ttttatttgt
    22621 ggtcattccc tgatattacg ggcactattt attcaaaact ctgacgaaaa acaggctgtc
    22681 gtcagttttg acgtgacgaa acgaaatacc gcgtgacagc catcacgcgg cagacatttt
    22741 attttttctc gacgacggga cttgcctgat acccgccgcc cagcgatttc atcaactgaa
    22801 tgctttggat cacccggcgg ctgtccagca tcagtaatga catctcttcg gcaagtactg
    22861 gcaaccgggc ttcggtggcc tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg
    22921 cagcgcgcag ggtaaagcgc gttgcttcca cgcgttcagc ctgcatctct cgctcgtcgt
    22981 tgagcgtttg cagacgcgtg ccgttgacgg caacgtcgcg caccgcattc agtactgact
    23041 ggttgtaacg ttcaatcatc atgttgctgg cggcgcgagt gccttcgaga ttggcattca
    23101 accgtccacc gtcaaacagc ggcaatttca gacccgggat aaagttgaac tggcgactgg
    23161 tttttttgaa taaggtatcc aggtggatag agtccaggcc gaaaaacgct ttgatatcaa
    23221 agctcggata gaacagcgcc cgcgcggaat ccacctgatc taatgacgcc tgaacatacc
    23281 agcgcatcgc ttgcagatcc gggcgtctgg cgagcaactc ataagagagt gtcgccggaa
    23341 tgccggtctg gacttgcggt aatgccaccg gtttgatctc cggcatatcg ctggctcccg
    23401 cgccaatcaa tgcacgcaga gattctcgcg tttcggtgat ttgcccttta acggcaacaa
    23461 tttgtttatc gaccgccaga atctgtgccc gcgcgccgtg gaaaggcact tgcgcttcca
    23521 gaccgtgcgc cactttactc tggtgcgctt tcaccgcgta atcaatcaca tcgcgtgttt
    23581 gttctaacag atcgagcatc tgatagctgg cctgcatact gtaataaagc tgcgctacgc
    23641 ccgtggtcag cgatagctct actgctgcgg tttctgccag cgcggcatta tgcgcgccaa
    23701 tggcggcagc aaccgctgag cgatgcacac cccacaaatc gagatccagt ccggcaaaca
    23761 aaccgactgt ggcttccgta tagaacggcc cgtccatacc cagtgctggc gcatccatcg
    23821 cataagggct taaaaagccg ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg
    23881 cgacctgtaa ttgtgaaccg gcatctaaca aatcggcctg cgactgcgct ttttcttccc
    23941 gcagtttcgc ttcggcgagg gtgtgtgaac cacttagcgt ccgttggatc agggcatcca
    24001 actgcgggtc attgagttgt ttccaccact gcgcctgcgg ccagccggag ctggcaagat
    24061 gaatatcatc ggccagtttg atttgttccg gtttgagctg ttgatgaggc gcagaatcct
    24121 tacgtaccag ggcacagccg gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca
    24181 ggcgtgaaag ttgacgattg atcattgcgt ggctccttgt acctgttccg tggcgggcgt
    24241 taatgccggg gctgtccagt ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg
    24301 ttttaataag gtcggcagga tggcctgcga ggcgggtgtt tcttcaagcg ttatttgagg
    24361 agaacgactg agtgcggttg ccagaccggt ggcgtatttc tccagtgcgt cggcaaactg
    24421 acctgctctg tccggctgta gtgcgttatc cagcgcctgc gccgagttcc aggttgcatc
    24481 ccaggcgtga agaatatcgc ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa
    24541 agcgcgttct tcgctgtcca gttgacgctc cagcgccacg cgttggcaca tctcttcaca
    24601 ggcattaaac gccgcatgca gacctatgcg aatttgcaga taagtgcgca gagctgtgac
    24661 ttcctgctgg cgtgggatcc gcattacttt actcaacata cccagcgcgc cagccagttt
    24721 ttgcggcagc gtgcgcgctt cgctttcagg ccagacaaag gtgtaaatca ccgccgacac
    24781 cacggtacca atgatgatac ccagagcgcg atcgcgaatt tccaccaggt cgtacaccgg
    24841 gccaaaaacg ttttcgagcg tggcgagcgc gaaggtgacc accatctgtg tgccgatata
    24901 agaagagcgt tcagagctgg tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac
    24961 aaacagcaat tcgacaatat tgtccagcca gggcatgacc aataacgtga ataacagcgc
    25021 cagaatcgcg ccgcaaaagg ccccgccaaa acgcagcacc atcttctggt acgacgaacc
    25081 gacattggga ttagcgacga tcacgcaggt cagcatacag gtgtgaatgc cttcccagtc
    25141 cacgccgctg tagaaggtgt aacagatcaa acaggcgagc agcgttttta ccgcgtagcg
    25201 catatagtct ggattggtaa aagcgtcggc gaccattgat ggcggtttgg tggcgggcgt
    25261 tggcggcgta ttcgggtcca tctgacccag ttgcaataac gtctggcaga tattctccag
    25321 gttacattcc cgtgccgcca tcgcttcact ttcagtgatc cgccagtcgc tttgccagca
    25381 ctgaccttcg gcaatggcat gctgcagctt gttgatttct gaggctaatt ttttccgaaa
    25441 ttcaataatt gcctgagaat cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta
    25501 aatgtaggtt accgttgcca cgcagctttg ccaccatgcg ctctgagttc gccagttggc
    25561 atcgtccgcg aggcaaaaga cattgagttt ttgcagcgcc agcgcctctc tttcaatccg
    25621 cgtttcgggt agcggtgcga ggctgtccgt caggtggcta atggcatcat caagccgatc
    25681 attaagcgcc tgatgcatct gcgtgatagc gcggctggga aaccacagca cgccgattaa
    25741 cgtcatcagc aaggttggat agaggccgac aacgatacac cacagggtta agcgcacgac
    25801 cacttccgga tagtcgagca tggcggggaa ggtttgcccg taaatagcga ctatggcgac
    25861 ggcgaaaaag accagcccca accgatgggt gcgcatcaaa aacatacagc ccatcaggat
    25921 cggtccggcg atgatcaatc ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa
    25981 caggctgccg atctccagca ccgtggcaac cacaaacaag atcgcgacaa atttggtgta
    26041 aaacgcgttc gactgaatac cgtaaaacag cactgccagc gataacgcca caaaagggat
    26101 ctcaaaggtc atcgagatca gaattaccag caggcagcct acccagagtt gcacggtctg
    26161 cggcacgcga cctggccgcc gctcgcttaa ctcttcatga aagaacgcca gcagcctgac
    26221 caccggtaat ggcagggagt tgagcgcgct cattattgcg gctcaagatt agcgactgcc
    26281 gaagcgccga tgcggaacat ttccgggtca ggtttatcga ccatgatttt gaccggaaaa
    26341 cgctgggcaa cgcggaccca gttaatagaa cgagacactt tcggcaggcc gcccagcacc
    26401 aggccgccgt catccggtag cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt
    26461 ttgccgctgt cgctcatcag gcgaatggtt gcgggtgtac ctgagcgaat atttttcaga
    26521 tcggtttcgc ggaagttggc gatcacgtac cagtgacgag tgtcgattag ggtaaaaata
    26581 gggcgcatgg cagaagcaaa ttgcccgacg gaggttttga gggaaatgac ccggccatca
    26641 aacggcgcgc gaacggtcgc catttccaga tgcagtttgg tcagggcaat atccgcttcg
    26701 accgccgcac gttgcgccac cagcgcatcg atgccgctga cggcgctggc ggctgactgc
    26761 gcctgtaaca atacggcatt aagatccgct tctgcggcgc gctgcgcagt tcttgcacgg
    26821 tcaacgtctt ccgctgagac aaaaccttct ttcagtaatg gctcggtgcg gcgtaatgta
    26881 tctgtggcct gtttcgcggc ggcacgggct ttttctaccg tggcattaac cgagtcggca
    26941 ccaaactgtt gcgcgtcaac gctacgctgg gtgagcataa tttgcttatc cagcgccgcg
    27001 agggaggctt cggctttcgc cagattggct tcgtatgggc gtgggtcgat gcggaatagc
    27061 aaatcaccct gtttcaccgc ctggttgtcg gtgaccgcca gttctacaat gcgaccgctg
    27121 acttccggca ccacatcaat ggtatctgct gacgcgtaag cgtcattagt tgatggcgca
    27181 ctgtctacgc gccagataac gaaaacaagg gcaaccagcg ccaacgcaac cactaacaga
    27241 gcagggaatt tactgcgagg agcttttttc ggcgtacttt ccatgagcgc gtctcaaata
    27301 gattagaaaa atgccagcca gaacagcatg gcgtagagag caaaaagggc ggtataaata
    27361 attccgacaa atgccagatt gatatttgcc cgctgaataa ttcgcctcgt gatgagcgtc
    27421 aaaataaggc tggcaatggc gcagaaaaac cagccgggat aataagcgcc gatcaccggg
    27481 attgccggag ataatgagca tcccgaaacc atgaagacgg gaattatcca ggctgttttt
    27541 ttgagttgca tcgccatgcg agagagaact gttggcatat taaatacatc ctgtctgctg
    27601 cgaacaatat ctgtagtgaa tatcaatatc cattgaaggc gtca
//
LOCUS       DAZKGF010000053        27549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZKGF010000053 DAZKGF010000000
VERSION     DAZKGF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.053"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            39..482
                     /gene="cmtB"
                     /locus_tag="ACHOQV_002122"
     CDS             39..482
                     /gene="cmtB"
                     /locus_tag="ACHOQV_002122"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFP9630496.1"
                     /translation="MRLIDYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILNQLLTAASEKQLADIISRG"
     gene            510..1898
                     /gene="cmtA"
                     /locus_tag="ACHOQV_002123"
     CDS             510..1898
                     /gene="cmtA"
                     /locus_tag="ACHOQV_002123"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFP9630497.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSVI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKN"
     gene            1913..3190
                     /gene="yggP"
                     /locus_tag="ACHOQV_002124"
     CDS             1913..3190
                     /gene="yggP"
                     /locus_tag="ACHOQV_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9630498.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTDKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3187..3502
                     /locus_tag="ACHOQV_002125"
                     /pseudo
     CDS             3187..3502
                     /locus_tag="ACHOQV_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            3524..4033
                     /gene="fumE"
                     /locus_tag="ACHOQV_002126"
     CDS             3524..4033
                     /gene="fumE"
                     /locus_tag="ACHOQV_002126"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFP9630499.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSRMVKTALTLAV
                     TTTLKELTP"
     gene            4030..4743
                     /gene="yggC"
                     /locus_tag="ACHOQV_002127"
     CDS             4030..4743
                     /gene="yggC"
                     /locus_tag="ACHOQV_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFP9630500.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WQELASFCDFSIFIHAPAQILRERLISRKIAGGLSRQEAEAFYARTDGPNVERVLVNS
                     RQANLILEMTEEGRYHFKS"
     gene            5028..6047
                     /gene="epd"
                     /locus_tag="ACHOQV_002128"
     CDS             5028..6047
                     /gene="epd"
                     /locus_tag="ACHOQV_002128"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFP9630501.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6097..7260
                     /gene="pgk"
                     /locus_tag="ACHOQV_002129"
     CDS             6097..7260
                     /gene="pgk"
                     /locus_tag="ACHOQV_002129"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP9630502.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            7474..8553
                     /gene="fbaA"
                     /locus_tag="ACHOQV_002130"
     CDS             7474..8553
                     /gene="fbaA"
                     /locus_tag="ACHOQV_002130"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9630503.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            8744..9604
                     /gene="mscS"
                     /locus_tag="ACHOQV_002131"
     CDS             8744..9604
                     /gene="mscS"
                     /locus_tag="ACHOQV_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP9630504.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            9743..10378
                     /gene="argO"
                     /locus_tag="ACHOQV_002132"
     CDS             9743..10378
                     /gene="argO"
                     /locus_tag="ACHOQV_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFP9630505.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIVHAQALLS"
     gene            10471..11211
                     /gene="yggE"
                     /locus_tag="ACHOQV_002133"
     CDS             10471..11211
                     /gene="yggE"
                     /locus_tag="ACHOQV_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFP9630506.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            11379..12275
                     /gene="ygfI"
                     /locus_tag="ACHOQV_002134"
     CDS             11379..12275
                     /gene="ygfI"
                     /locus_tag="ACHOQV_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9630507.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(12272..13750)
                     /gene="scpC"
                     /locus_tag="ACHOQV_002135"
     CDS             complement(12272..13750)
                     /gene="scpC"
                     /locus_tag="ACHOQV_002135"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFP9630508.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPNAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(13774..14559)
                     /gene="scpB"
                     /locus_tag="ACHOQV_002136"
     CDS             complement(13774..14559)
                     /gene="scpB"
                     /locus_tag="ACHOQV_002136"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFP9630509.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(14570..15565)
                     /gene="meaB"
                     /locus_tag="ACHOQV_002137"
     CDS             complement(14570..15565)
                     /gene="meaB"
                     /locus_tag="ACHOQV_002137"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFP9630510.1"
                     /translation="MINEAKLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNRLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(15558..17702)
                     /gene="scpA"
                     /locus_tag="ACHOQV_002138"
     CDS             complement(15558..17702)
                     /gene="scpA"
                     /locus_tag="ACHOQV_002138"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFP9630511.1"
                     /translation="MSKVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSAAE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGRKDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(17906..18799)
                     /gene="argP"
                     /locus_tag="ACHOQV_002139"
     CDS             complement(17906..18799)
                     /gene="argP"
                     /locus_tag="ACHOQV_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFP9630512.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            18940..19125
                     /locus_tag="ACHOQV_002140"
     CDS             18940..19125
                     /locus_tag="ACHOQV_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630513.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFASWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            19181..19840
                     /gene="rpiA"
                     /locus_tag="ACHOQV_002141"
     CDS             19181..19840
                     /gene="rpiA"
                     /locus_tag="ACHOQV_002141"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP9630514.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            20096..21328
                     /gene="serA"
                     /locus_tag="ACHOQV_002142"
     CDS             20096..21328
                     /gene="serA"
                     /locus_tag="ACHOQV_002142"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9630515.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLAEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            21557..21616
                     /gene="ibsC"
                     /locus_tag="ACHOQV_002143"
     CDS             21557..21616
                     /gene="ibsC"
                     /locus_tag="ACHOQV_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFP9630516.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(21717..22319)
                     /gene="fau"
                     /locus_tag="ACHOQV_002144"
     CDS             complement(21717..22319)
                     /gene="fau"
                     /locus_tag="ACHOQV_002144"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP9630517.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTLPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(22340..22523)
                     /gene="ssrS"
                     /locus_tag="ACHOQV_002145"
     ncRNA           complement(22340..22523)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHOQV_002145"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22565..22894)
                     /gene="zapA"
                     /locus_tag="ACHOQV_002146"
     CDS             complement(22565..22894)
                     /gene="zapA"
                     /locus_tag="ACHOQV_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9630518.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALFEQGRITE
                     KTNQNFE"
     gene            23062..23640
                     /gene="ygfB"
                     /locus_tag="ACHOQV_002147"
     CDS             23062..23640
                     /gene="ygfB"
                     /locus_tag="ACHOQV_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFP9630519.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            23666..24991
                     /gene="pepP"
                     /locus_tag="ACHOQV_002148"
     CDS             23666..24991
                     /gene="pepP"
                     /locus_tag="ACHOQV_002148"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFP9630520.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            24988..26166
                     /gene="ubiH"
                     /locus_tag="ACHOQV_002149"
                     /gene_synonym="visB"
     CDS             24988..26166
                     /gene="ubiH"
                     /locus_tag="ACHOQV_002149"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFP9630521.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGCVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWGPLVVGRNIGLMAMELFTPARDVLAQRTLGWVAR
                     "
     gene            26189..27391
                     /gene="ubiI"
                     /locus_tag="ACHOQV_002150"
                     /gene_synonym="visC"
     CDS             26189..27391
                     /gene="ubiI"
                     /locus_tag="ACHOQV_002150"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFP9630522.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(27465..27548)
                     /locus_tag="ACHOQV_002151"
     CDS             complement(27465..27548)
                     /locus_tag="ACHOQV_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630523.1"
                     /translation="MSKNTKSKIHNIRKYNAKTEVKLVYFK"
BASE COUNT         6577 a         6754 c         7364 g         6854 t
ORIGIN      
        1 cgccggacgg tgccctaaaa ataaaataag gattatttat gcggcttatt gattattttc
       61 cagaatcatc aatctcagtt atacattcag caaaagattg gcaggaagct atcgatttct
      121 cgatggcatc attgctggat aaaaactata tcagcgagaa ttacattcag gcaattaaag
      181 attccaccat taacaatggc ccttattata ttctcgcacc aggcgtggca atgcctcatg
      241 ctcgaccgga atgtggggcg cttaaaaccg ggatgtcttt aacattactt aaacaaggcg
      301 tttattttcc ggggaatgac gaaccgataa aattactcat cgggctttct gctgccgatg
      361 ccgattcgca cattggcgct attcaggcgt taagtgaatt attgtgtgaa gaagaaatac
      421 tcaaccaatt attaacagca gcatcagaaa aacaattagc ggacataatc agccgcggat
      481 aattactctc tcctctacag gataatatta tggaaaacaa gtctgctcgt gcaaaggtcc
      541 aggcttttgg gggctttttg actgcaatgg tcatccccaa tatcggcgct tttatagcct
      601 ggggttttat aacggcgtta tttattccca ccggctggct gcctaacgaa catttcgcca
      661 aaattgtcgg cccgatgatt acctatttat tgcccgtgat gattggttct accggtggtc
      721 atctggtcgg cggtaaacgc ggcgcggtca tgggcggaat aggtactatt ggtgtgatcg
      781 tcggcgcaga gatcccgatg ttccttggct caatgattat ggggccgctc ggtggtctgg
      841 tgattaaata tatcgacaag gcgctggaaa agcggatccc tgctggtttt gagatggtta
      901 tcaataactt ctcattaggt attgcgggga tgctcctttg tctgctgggt tttgaagtta
      961 tcggcccggc ggtgttaatt gccaatactt tcgtcaaaga gtgtattgag gcgttggtac
     1021 atgcgggtta tctgcctttg ctgtcagtca tcaatgaacc ggcgaaagtg cttttcctca
     1081 ataacgcgat agatcagggc gtctattacc cgttggggat gcaacaggct tcagttaacg
     1141 gtaaatccat cttctttatg gtggcgtcca accctgggcc tggcttagga ctgttgctgg
     1201 cgtttacttt gtttggtaaa gggatgagta aacgttctgc gcccggggcg atgattattc
     1261 acttcctcgg tgggatccac gaactgtatt tcccgtatgt gctgatgaag ccgctgacca
     1321 ttattgccat gattgcgggc ggtatgtctg gcacctggat gtttaactta ctggacggtg
     1381 gtctggtggc tggcccaagt ccggggtcta tctttgctta cctggcactg acgccgaaag
     1441 gctcgttcct ggcgacaatt gccggtgtta cggtaggtac cctggtgtcc tttgctatca
     1501 cttccgtaat actgaagatg gaaaaaacgg tggaaacgga gagcgaagat gagtttgctc
     1561 agtcagccaa tgcggttaag gcgatgaaac aagaaggtgc attctcgtta agcagggtta
     1621 agcgtatcgc ctttgtttgc gatgcgggga tgggctccag cgcgatgggc gcgaccacct
     1681 tccgtaaacg cctggaaaag gcggggctgg caatagaagt aaaacattac gcgatagaaa
     1741 acgtgcctgc ggatgcggat atcgttgtta ctcatgccag tctggaaggg cgcgtgaaac
     1801 gtgtgacgga taaaccgctg atactgatta ataactatat tggcgatcca aaactcgaca
     1861 ctttatttaa tcaattaacc gccgaacata aaaactgatt gccgaggtaa aaatgaaaac
     1921 caaagttgct gctatttatg gcaagcggga tgtccgtctg cgcgaatttg aactgccaga
     1981 aattaccgat aatgaactgc tggtgagtgt gatttctgac agcgtgtgtt tatccacctg
     2041 gaaagcggcg ctactcggta gtgaacataa acgcgtaccc gacgatttag aaaatcatcc
     2101 ggtcattacc gggcatgaat gcgccggggt tattgtcgaa gtgggtaaaa atctcaccga
     2161 caaatataaa aaaggccagc gttttgtatt gcaaccggca atggggttac caagcggata
     2221 ttcagcaggc tacagctacg aatattttgg cggtaacgcc acctatatga ttattcctga
     2281 aatagccatt aacctgggct gcgtattacc gtatcacggc tcttattttg ctgcggcgtc
     2341 gctggcagag cctatgtgct gcattattgg cgcttatcat gccaattatc acaccacgca
     2401 atatgtttat gagcatcgca tgggcgttaa acctggcggc aatattgcgc tgctggcatg
     2461 tgctggtccg atgggcattg gcgctatcga ttatgctatt aacggcggca tacaaccgtc
     2521 gcgggtggtg gtggtcgata tcgacgacaa acgcctggcg caggtacaaa agctgctgcc
     2581 ggtggaactg gcggccagta aaggcattga gctggtgtat atgaatacca aagggatgag
     2641 cgatccggtc cagacgctgc gggcgctgac ggatgatgcc ggatttgatg acgtatttgt
     2701 ctatgcagcg gtgccttcag tcgtcgagat ggctgatgaa ttactggcgg aagatggctg
     2761 cctgaacttc tttgccgggc cgacggataa aaacttcaaa gtgccgttta atttctacaa
     2821 cgtccattac aacagcacgc atgtcgtcgg tacgtccggc ggttcaacgg acgacatgaa
     2881 agaggcgatt gcgcttagcg ccacagggca gttgcagccg tcgtttatgg tgacccatat
     2941 cggtgggctg gatgcggtgc ctgataccgt gctcaatctg ccggatatcc ccggcggtaa
     3001 aaaactcatt tataacggcg tcaccatgcc gctcactgcc attgccgatt ttgccgaaaa
     3061 aggcaaaacc gatccgctgt ttaaagagct ggcgcggctg gttgaggaaa cgcacggcat
     3121 ctggaatgag caggccgaga aatatctgct ggcgcaattt ggcgttgata tcggggaggc
     3181 cgcgcaatga tgtccctggc gtggccatta tttcgcgtta cggaacaggc agcgttggct
     3241 gcctggccgc aaaccggatg tggcgacaaa aataaaattg atggcctggc ggtcaccgcg
     3301 atgcgccagg cattaaacga cgtcgctttt cgtgggcgag tggttatcgg cgagggggaa
     3361 cgatatcctc tttagcgcta cgggcgtgac gggcggcgag ttggtgaacg gtatccaaca
     3421 gacggcgaat ggggtgcgga cgcagacatt actgatcggc ggcgcggacc aaacgtgtaa
     3481 tataatagac tccctgcatt gatggtgatc tatcggaaga ccaatggcga cgctgacaga
     3541 agatgatgtg cttgagcaac tggatgcaca ggacaattta ttttcattta tgaaaactgc
     3601 gcattctatt ttgctccagg ggatacgcca gtttctgccg tcgctgtttg tcgataacga
     3661 tgaagagatc gtcgaatatg cagtgaagcc gttactcgcc caaagcggcc cgcttgacga
     3721 tattgatgtt gcgctgcgtt tgatttatgc gctggggaaa atggataaat ggctgtacgc
     3781 cgatatcacg catttttccc agttttggca ttacctgaac gaacaggatg aaacgcccgg
     3841 atttgccgat gacatgacct gggattttat cagcaacgtc aatagcataa cctgcaatgc
     3901 gacgctctat gacgcgttga aagcgatgaa gttcgccgat ttcgccgtct ggtcagaggc
     3961 gcgttttagc aggatggtca aaacggcgct gacgctggca gtaacgacca ccttaaagga
     4021 attaacgccg tgaaaattga attaacggtg aatgggctga agatacaggc acagtaccag
     4081 aacgaagaga ttgaaaatgt acataaaccg ttgctgcaca tgttggcggc cttacagacg
     4141 gtaaatccgc agcggcgtac ggtggttttt ctttgcgctc cgccggggac aggtaaatct
     4201 accctaacca ccttctggga atatctcgca caacaagacc cggaactgcc cgctattcag
     4261 acactgccga tggatggttt tcaccattac aatagctggc tggatgcgca tcaattgcgc
     4321 cccttcaaag gcgcaccaga gacattcaac gttgcgaaac tggcggaaaa tctgtgccgg
     4381 gttgtggaag gggattgtac gtggccgcag tacgatcgac aaaagcatga tcctgttgaa
     4441 gatgcgttgc acgttaccgc accactcgtc atcgtcgaag gaaactggct attactggat
     4501 gatgaaaagt ggcaggagtt ggcatctttc tgcgattttt ctatttttat tcatgcacct
     4561 gctcaaattc tgcgggagcg cttgattagt cgtaaaattg cgggaggatt atcgcggcaa
     4621 gaagccgaag ctttttatgc ccgaaccgat ggtcccaatg tcgagcgtgt gctggtgaat
     4681 agtcgtcagg ccaatctgat attagagatg accgaagaag gcagatatca cttcaaaagc
     4741 tgacaattta ttccagttat tgcaggggta ttattacgcc agcctggaat aaagccccct
     4801 acaaaagggg ataaaagtgt gatgtaagtc agataaatgt cttcttcggc tggacaatca
     4861 ttccttttat tccacgtttc gcttatccta gctgaagcgt ttcagtcgat taaatgttcg
     4921 acaattaacc aatcagtcgc agtttgcgac aggtaaggtt tccccggacg atttgctgga
     4981 ttactctgtc tggcaaattt gctcagggaa aaccttgcag gagatctatg accgtacgcg
     5041 tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt tcgtgctttg tatgaatccg
     5101 gacgccgggc ggaaattacc gtggtggcaa tcaacgaact ggcggatgct gcgggcatgg
     5161 cgcatttgtt gaaatatgac accagccatg gccgttttgc atgggaagta cgacaggaac
     5221 gcgatcaact ttttgttggt gatgacgcca tccgtgtatt gcatgaacgt tcactgcaat
     5281 cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga ctgcaccggc gtatatggct
     5341 cccgcgagca tggcgaagcg catattgccg ccggggccaa aaaagtgctc ttttcacatc
     5401 caggcagtaa cgatctcgac gcgaccgttg tttacggcgt caatcaggat caacttcgtg
     5461 cggaacaccg catcgtttct aacgcttcct gtaccacgaa ttgcataatt cccgtcatca
     5521 aattgttaga tgatgcgtac ggtattgagt ccggcactgt gaccacaatt cactccgcca
     5581 tgcacgatca acaggttatt gatgcatacc atcctgacct gcgtcgcacc cgggctgcca
     5641 gccagtcgat cattccggtc gatactaaac tggccgccgg tatcacacga ttttttccgc
     5701 aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc aaccataaat gtgacggcaa
     5761 tcgatttaag cgtgacggtg aagaaacctg taaaagccaa tgaagtcaac ctgttgctgc
     5821 aaaaagcagc acaaggtgca tttcatggta tagttgacta tacggaattg ccgttggtct
     5881 ctgtagattt taaccacgat ccgcacagtg ccattgtcga tggcacccaa acccgggtta
     5941 gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa cgaatggggc tttgctaacc
     6001 gaatgctcga cacgacgtta gctatggcta ctgttgcttt caggtaagac gcaagcagcg
     6061 tctgcaaaac ttttagaatc aacgagagga ttcaccatgt ctgtaattaa gatgaccgat
     6121 ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc tgaacgtacc agtaaaagac
     6181 gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc cgaccatcga acttgccctg
     6241 aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc gtcctaccga aggcgagtac
     6301 aacgaagaat tctctctgct gccggttgtt aactacctga aagacaaact gtctaacccg
     6361 gttcgtctgg ttaaagatta cctcgacggc gttgacgttg ctgaaggtga actggttgtt
     6421 ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg acgaaaccct gtccaaaaaa
     6481 tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg gtactgctca ccgcgcgcag
     6541 gcttctactc acggtatcgg taaattcgct gacgttgcgt gcgcaggccc gctgctggca
     6601 gctgaactgg acgcgctggg taaagcactg aaagaacctg ctcgcccgat ggtggctatc
     6661 gttggtggtt ctaaagtatc taccaaactg accgttctgg actccctgtc taaaatcgct
     6721 gaccagctga ttgttggtgg tggtatcgct aacaccttta tcgcggcaca aggccacgat
     6781 gtgggtaaat ccctgtacga agctgatctg gttgacgaag caaaacgtct gctgaccacc
     6841 tgcaacatcc cggttccgtc tgatgttcgt gtagcaactg agttctctga aactgcaccg
     6901 gctaccctga aatctgttaa cgatgtgaaa gctgacgagc agatcctgga tatcggtgat
     6961 gcttccgctc aggaactggc tgaaatcctg aagaatgcga aaaccatttt gtggaacggt
     7021 ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta ctgaaatcgt ggctaatgct
     7081 atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg acactctggc agcaatcgac
     7141 ctgttcggca ttgctgacaa aatctcctac atctccactg gcggcggtgc attcctcgaa
     7201 ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg aagagcgcgc taagaagtaa
     7261 aaaatcacag ggcagggaaa cctgcccttg ttgcagcgcg cttttaaagc acgcactttt
     7321 tcatactcta aataattcga gttgcaggaa ggcgacaaga gagtgaatcc catgagctta
     7381 cataagtaag tgactggggt gagcgaacgc agacgcagca catgcaactt gaagtatgac
     7441 gagtataagg cccgacgata caggacaaga gacatgtcta agatttttga tttcgtaaaa
     7501 cctggcgtaa tcactggtga tgacgtacag aaagttttcc aggtagcaaa agaaaacaac
     7561 ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca tcaacgccgt gctggaaacc
     7621 gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca acggtggtgc ttcctttatc
     7681 gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg ctatcctggg cgcgatctct
     7741 ggtgcgcatc acgttcacca gatggctgaa cattatggtg ttccggttat cctgcacacc
     7801 gaccactgcg cgaagaaact gctgccgtgg atcgacggtc tgctggacgc gggtgaaaaa
     7861 cacttcgcag ctaccggcaa gccgctgttc tcttctcaca tgatcgacct gtctgaagaa
     7921 tctctgcaag agaacatcga aatctgctct aaatacctgg agcgcatgtc caaaatcggc
     7981 atgactctgg aaatcgaact gggttgcacc ggtggtgaag aagacggcgt ggacaacagc
     8041 cacatggacg cttctgcact gtacacccag ccggaagacg ttgattatgc gtataccgag
     8101 ctgagcaaaa tcagcccgcg tttcaccatc gcagcgtcct tcggtaacgt acacggtgtt
     8161 tacaagccgg gtaacgtggt tctgactccg accatcctgc gcgactctca ggaatatgtt
     8221 tctaagaaac acaacctgcc gcacaacagc ctgaacttcg tattccacgg tggttccggt
     8281 tctactgctc aggaaatcaa agactccgta agctacggcg tagtgaaaat gaacatcgat
     8341 accgataccc aatgggcaac ctgggaaggc gttctgaact actacaaagc gaacgaagct
     8401 tatttgcagg gtcagctggg taacccgaaa ggcgaagatc agccgaacaa gaaatactac
     8461 gatccgcgcg tatggctgcg tgccggtcag acttcgatga tcgctcgtct ggaaaaagca
     8521 ttccaggaac tgaacgcgat cgacgttctg taagtcattc ctttctgctt atctcaaggc
     8581 ccgctctgcg ggtctttttt tcgccaaaag caaggtgatg cagatgagaa atctgtgatc
     8641 tatttggcaa aattatgctt tattgtttac ccttgtcaga ctgcccgcca taaggcggcg
     8701 gagtgtattt ctccattttg agtcagttga aaaggaatat tgaatggaag atttgaatgt
     8761 tgtcgatagc ataaacggcg cgggaagctg gctggtggct aaccaggcgc tgctgctaag
     8821 ttatgcagta aacatcgtgg cggcactcgc gatcatcatt gttggtttga ttatcgcgcg
     8881 gatgatttcc aacgcggtga atcgcctgat gatctcccgt aaaatcgatg ccactgttgc
     8941 tgattttctc tctgcattag tccgttacgg tattatcgcc tttacgctaa tcgctgcact
     9001 ggggcgcgtg ggcgtacaaa cagcgtcagt cattgctgta ctcggtgccg caggcttagc
     9061 ggttggtctg gctttgcagg ggtcactgtc taacctggcc gctggcgtgt tacttgttat
     9121 gttccgcccg ttccgtgccg gagaatatgt tgatctcggc ggcgtagccg gtactgtgct
     9181 gagtgtgcag attttctcca ccaccatgcg tactgcagac ggtaaaatta tcgttattcc
     9241 gaacggtaaa attattgccg gaaatattat taacttctct cgcgagccag ttcgccgtaa
     9301 cgaatttatt attggcgtgg cgtatgattc cgatatcgat caggttaagc agatcctgac
     9361 cgatattatc cagtctgaag atcgcatttt gaaagatcgc gaaatgactg tgcgcctgaa
     9421 cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc tggagcaaca gcggcgatct
     9481 gcaaaacgtg tactgggatg tgctggagcg tattaaacgt gaatttgatg ccgccggtat
     9541 cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg gtgaaagaag acaaagctgc
     9601 gtaatcaacg ctgaggccag ataatactct ctggcctctc tcttattagt ttttctgatt
     9661 gccaattaat attatcaatt tccgctaata acaatcccgc gatatagtct ctgcatcaga
     9721 tacttaattc ggaatatcca acgtgttttc ttattacttt caaggtcttg cacttggggc
     9781 ggctatgatc ctaccgctcg gtccacaaaa tgcttttgtg atgaatcagg gcattcgtcg
     9841 tcagtaccac attatgattg ccttactttg cgctatcagc gatttggtcc tgatttgcgc
     9901 cgggattttt ggtggcagcg cgttattgat gcagtcgccg tggttgctgg cgctggtcac
     9961 ctggggcggc gtagccttct tgctgtggta tggttttggc gcttttaaaa cagcgatgag
    10021 cagtaatatt gagttagcca gtgccgaagt cctgaagcaa ggcagatgga aaattatcgc
    10081 caccatgctg gcagtgacct ggctgaatcc gcatgtttac ctggatactt ttgttgtact
    10141 gggcagtctt ggcgggcaac ttgatgtgga accaaaacgt tggtttgcgc tcgggacaat
    10201 tagcgcctct ttcctgtggt tctttggtct ggctattctc gcagcctggc tggcaccgcg
    10261 cctgcgcacg gcaaaatcac agcgcattat caatctggta gtgggatgtg ttatgtggtt
    10321 tattgccttg cagctggcga gagacggtat tgttcatgca caagccttgc tcagttaggc
    10381 gctatctgat ggaaaaataa aacagaggcg ctaagcttgc ctccagaggt cctgaatttt
    10441 aggcaagtca attaacacgg agagactaac gtgaagttca aagttatcgc cctggcggca
    10501 ttaatgggta ttagcgggat ggcagcgcag gctaacgaat tgccggatgg accgcatatt
    10561 gtcacctccg gtacggcaag cgtggatgcg gtgccagaca ttgccactct tgcgattgaa
    10621 gtcaacgtgg ccgcgaagga tgccgctact gccaagaaac aggcggatga gcgcgtcgca
    10681 caatacattt ccttccttga actcaatcag atcgcgaaaa aagatatcag ctcagcgaac
    10741 ttacgcaccc agccagatta tgattatcag gatggtaaaa gtatcctgaa aggctaccgt
    10801 gctgtgagaa cggtggaagt cacgctccgt cagttagaca aactgaattc cttgctggat
    10861 ggcgcgctga aggcgggtct taacgaaatt cgttctgtat cgctgggcgt ggcgcagccg
    10921 gatgcctata aagacaaagc gcgtaaggca gcgattgata acgcgattca tcaggcgcag
    10981 gaactggcga acggctttca tcgtaaactg gggccggtat atagtgtgcg ctaccatgtt
    11041 tccaactatc agcccagccc aatggtgcgg atgatgaaag ccgatgccgc gccggtgtcc
    11101 gcccaggaaa cttacgagca ggccgctatt cagtttgatg atcaggtcga tgtggtcttc
    11161 cagttagaac ctgtggatca acaacccgct aaaacacctg cagcacaata atatttgtag
    11221 cccgacgtat tcgtcgggct aatgccttta cccgatattg cctccttttt ctgtaatttt
    11281 ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt ttcatcaagg ataggctgac
    11341 tgaaacggtt aagcggacaa ggatgcgggg gagggggtat ggacattttt atttcgaaaa
    11401 agatgcgcaa tttcatttta ttagcgcaaa ccaataatat tgcccgggcg gcagaaaaaa
    11461 tccatatgac agcttcgcca tttggtaaaa gcattgccgc gctggaagaa caaattggct
    11521 atacgctatt tacccgcaaa gataataata tcagcctcaa taaagccgga caggaactgt
    11581 atcaaaaact gttcccggtt tatcaacggc tttctgcgat tgataatgaa atccataact
    11641 cagggcgtcg ttcacgagaa attgtgattg gcatagataa tacttatccg acaattattt
    11701 tcgatcagtt gattagtctc ggagataagt atgaaggcgt gaccgcccag ccagttgagt
    11761 tcagcgaaaa tggcgttatc gataatcttt ttgaccgtca gttagatttt attatctccc
    11821 cacagcatgt atctgcacga gttcaggaac tggaaaacct gactatcagt gaactaccgc
    11881 cgctgcgatt gggctttctc gtttctcgtc gttatgaaga gcgacaagag caagagttgc
    11941 ttcaggagct accctggcta caaatgcgct ttcagaaccg ggctaacttc gaagcaatga
    12001 ttgatgcgaa tatgcgtcct tgtgggatca atcccaccat tatttatcgt ccctacagtt
    12061 ttatggccaa aatcagcgcc gtggagcgcg gacattttct gacggtgatc ccgcattttg
    12121 cctggcgact ggtgaatccg gcaacgctga aatattttga tgcgccgcac aggccgatgt
    12181 atatgcagga atatctttat tcaatcagaa atcatcgtta taccgccacg atgcttcagc
    12241 atattgctga agatcgtgac gggacgagtc attaacccag catcgagccg gttgcaatta
    12301 aattacggtg taagtcgaag acgtggctaa gatcgtggtg aatatgtccg ccgggcgcat
    12361 tttccagata gcgatgcaga taatcccgat acataggatg tgcacaatta tcaatgatag
    12421 tgcgggcgcg ttgaagcggg gaaagaccac gcagatcggc gatgccttgt tcggtgatga
    12481 tcactttgac gctgtgttcg ctgtgatcaa catggctgca cattggcacg acggttgaga
    12541 tcttgccttc tttagcaatc gacggggcca taaagatcga cagatacgcg ttgcgttcaa
    12601 aatcaccgct gccaccgatg ccgttcatca gatcgacccc ggctacgtgc gttgagttgg
    12661 catgcccgta aatatcaaac tccaggccga cgttcagagc gatgacgccc agacgacgga
    12721 tgatttccgg gttattggaa atctcctgcg gacgcaacac aatgcggctg gcaaagtaat
    12781 ccatattgtc gtaaatcttg cgcagggaat cggccgagat agtcaggctg gaggcgctgg
    12841 ccccgctgat tttgcccgtt tccagtaaat gcaccaccga ttcctgtagc acttccgaat
    12901 acatcataaa cggaggaatt tccggatttt cccccagacg cgccattacc gcattattga
    12961 tgttgcctac gccactttgc agcggcagaa attccggcgg aatacgccca tgcgccattt
    13021 cctgcaacaa gaacgtgacc acgttatcgg caatctgctg gcacatggga ttttgcttat
    13081 ccagcatatt accggcgtca ggcaagttgg tttccacgac ggcgacaatc tttttcggat
    13141 cgatttgcac atagcgggta ccgacgcgat ccattgcatg gaagatcgac acgctattgc
    13201 gccgcggtgg cgcgccagga atcacaatat ctgccagttc tgcaacgcgc ggatcgtgat
    13261 agtgattgag ttcaatgatc actttcttcg cccgcagcag ccaggtcggc gcattaccga
    13321 tcccgctggt taaccagact cgaccatccg gtgccagtgc cgatgcttca atgacggcaa
    13381 catcaatgtc gccgaagaaa ccgtaattga ccatttgcgc cacttcgctc aaatgcaggt
    13441 caacgaaact caccgcgccc tgattgatct ttttacgtaa accggacgat gtttgatatg
    13501 gcgcacgcca ggaaacagca tcggcgtcag aaagtacatc gtcagcggcg gcgctgattg
    13561 acgcccccgt cagcaggcga atttgatacg gctttttggc ctcatgctgt tcgttagctc
    13621 tgcgggcaat cgcgttgggt aaggctttcg gcgaacccgc tggggtaaag ccgctaaatg
    13681 ccaccatgtc gttatgctgg ataatttctg ccgcttcatc ggcggtcatc cttgtccact
    13741 gagtttccat tttaactcct tcgttcgcag ggattaatga ccaacgaaat taggtttacg
    13801 tttttcgagg aaagcgttca tcccttcctg gtaatcttcg ctgtcataaa ccgcgcggcg
    13861 catcccctga atacgttcaa attcatcgga gttcatggtg tgcgcttcgc ccagtacacg
    13921 cagctcttct ttgataacgg caatggctaa cggcgctttc tcagagatgt ggtgcgccat
    13981 ttgtaaggtg aaatcttcca gttcttccac ttccacaaca tggttgagga tgccgacagc
    14041 cagcgcgcgc tgggctgtga ttggcgaagc ggtaaaaatc agctctttga caatgtggaa
    14101 gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta tacgggacgc cgaggtttac
    14161 aggtgtcatt gagaaggttg aggtactggc ggcgatgatc agatcggaac tcatgatcat
    14221 ttcaaatgcg ccaccccaga cactaccttc caccatcgaa atgatcggtt tcgggaattt
    14281 ttggatcatg cgggtgattt gacgcaacgg atcatcatag gagagcggat cgcgaccgcc
    14341 agacggcagt tcgtgaatat cgtgacctgc ggagaagact ttggaaccac tcggcgcgcg
    14401 caaaatgata cagcgaattt ccggccggtt gagatcgctt aatgcctgca taagatcatc
    14461 aataaagact ttacttaagg cattaagttt gcggccatag ttaaactcaa tgaccgccac
    14521 tttgttgata gtgacaacgt tcacatactg ataagacata aaaattcctt taatcaaaat
    14581 attgcgtctg gataaattca ctgagctgcc gcaggccggt gcgcggtgag agcgtattgt
    14641 ttttgactgc taaaagcgtc tggcggtaat agcgatcgaa atcttcattc gcgaacaggc
    14701 gattcagtac ttcttcttcg gtctgcttac gcagccattc caccgattgt tgttgccgca
    14761 cttgttgtaa tcgaccactg gcagttagcg cggttttgaa gtcgatgatg gcgtgccaga
    14821 tctcatcgat tccacgtttt tccagtgcgc tacaagtcag aacccgtggc tgccattcgt
    14881 cgtatttacg tcgcagaata tgcagggcac tctcgtacat atgccgggca atggcgacat
    14941 tggtatggtt atcgccatcg tctttgttga taacgatcag atctgccact tccatcagcc
    15001 cctttttaat gccctgcaga tcatcgccgc caccggcaat ttgcaacgag ataaaacagt
    15061 ccaccatgcg ggcgacttcc gtttctgact gcccgacgcc aaccgtttcg acaatcacta
    15121 cgtcataacc cgccgcttcg cataacagca ttaattcccg cgctcgctga ctggcaccgc
    15181 ccagatggcc ggaggagggt accgggcgaa taaacgccgc ctcggcacgc gcaaggtcat
    15241 tcatgcgggt tttatccccg agaatgctac cgccagtgac cgggctgctg ggatcgaccg
    15301 caataaccgc aacctttaat ccctcacgaa tcaacaacat gccaaaggct tcaagaaagg
    15361 tacttttccc cgcgccgggt gtgccggtaa cgcccagtcg cagggcgtta ccacagaacg
    15421 gcataatggc gtcaagcagc tgtgtactta gcgcctgatg acgcgggtga cggctttcca
    15481 ccaacgtcat ggcctgggcg agcgtggcac gctcaccctg acgtaagcgg cgaatacttt
    15541 ctgctagctt ggcttcatta atcatgatgc tggcttatca gattcagtac gtcgcgcacg
    15601 ctgtcgagca taggtgtacc tggaccataa atcgccgcca cgccgcgctc ttgcaggaag
    15661 gcgtaatcct gcggcggaat gacgccaccc gcgaccacgc agatatcttt gcgtccccat
    15721 tttttcagcg cttcgaccag ttccgggatc agcgttttat gaccggcagc cagtgaggat
    15781 gcgcccacta cgtgaacgtc gttttctacg gccaggcggg cgatctcttc aggtgtagag
    15841 aacatcgggc ttaaatctac gtcgaaaccg agatcggaat aggcgctggc gatcactttc
    15901 gcgccgcgat cgtgcccatc ctggcccatt ttggcgatca gaatgcgcgg gcgacgacca
    15961 ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat cgaactcggc ggccgatttc
    16021 tcagactgat gatagctttg cgcaatcacg ccggtaacac actggcttgg caccagataa
    16081 cggtcgaaag cggcttccag cgcatcggaa atttcaccca gggtggcgcg aacgcgagca
    16141 gcattaacag cggcagccag caggttttcg ttatgctgtg cggcgtgagt cagggcgttc
    16201 aacgcggcgg ttacggcggc atcatcacgg gtggcgcgaa tgcgttccag cgaagcaatt
    16261 tgctcgttac gcaccatcac gttgtcgatc tcaagtacat cggtttcgtc ttcgtgatcc
    16321 agtttgtact tgttgacacc aacgatgaca cgcttgccct ggtcgatcag cgactgttcg
    16381 cgcgctgagg cctcttcgat cattcgtttt ggcagacctg cttcgatcgc tttcgccatg
    16441 ccaccggctt cgtcgatctg ttggataata gctctggctt gtttgacgat ttgatcggtc
    16501 agcgactcaa tgtaatagga tccggccagt ggatcgacgg tgcggcagag ttctgattct
    16561 tcctgaatga tgatctgggt attgcgggca atgcgtgctg agaaatcggt aggcaaacca
    16621 agcgcttcat caaaggcgtt ggtatgcagt gactgagtac cgcccagcgt tgcagccagt
    16681 gcttcaatgg tggtgcggat aacgttgtta tacggatcct gttcagtcag gctccagcct
    16741 gaggtctggc agtgggtacg cagcgccagt gatttcggat cctgtgcgcc aaatccactg
    16801 accgcttcgc tccataaata acgtgccgca cgcaacatgg cgacgttcat aaacagatcc
    16861 atgccaatgc cgaagaagaa cgacagacga ggagcgaagt catcaatttt cagcccggcg
    16921 gagattgctg ctttgatgta ctcaatccca tcggcgagcg taaatgctac ctgctgcacg
    16981 cagttggcac ccgcttcacc catgtggtaa ccgctgatac tgatggtatt aaatcgcggc
    17041 atgttgccgg aacaccaggc gatgatgtct gcgataatac gcattgacgg ttttggcggg
    17101 taaatatagg tgttgcggca aagatactct ttgaggatat cgttttgaat ggtgccggtc
    17161 agtttatcgg gtgtaacacc ttgctcttct gcggcgacaa tataaaacgc cagtactggt
    17221 agtactgcgc cattcatggt catcgaaacc gacattttat ccagcgggat ctggtcgaac
    17281 aggactttca tatcttccac ggtgtcgata gcaacgcccg ctttgccgac gtcgcccgcc
    17341 acgcgtgggt tatcggagtc gtagcctcgg tgggtggcaa ggtcaaacgc aacggaaaga
    17401 cctttttgcc cggcggccag gttacggcga taaaaagcgt tggactcttt tgctgttgaa
    17461 aaaccagcat actgacggat ggtccacggt tgggcggtat acatagtggc acgcgggcca
    17521 cgaacgtagg gaggcaaacc aggaagggta cctgtcacct ccagattatc gagatcggct
    17581 tcggtataca gcggcttgat ggcgatccct tccgcggttt gctgaaccag cgagtcgaca
    17641 gttttctccc gacggctcaa ttccttgttg gcaagctgtt gccactcctg caccttagac
    17701 attatttgct ccgtaaaacg caatcaaaat gagggtattt gcgcttacgg tgagcttttt
    17761 acgtgggttt gtcgccagca aaaaaaatgc attcagacgt cgttttttgc cgcctgagat
    17821 ttgtctattt tgtgatgtga ttcaaggaat acaaaaagaa gtgaggataa cgcctgatat
    17881 gtgctatcag gcattatttg atggattaat cctgacgaag gactttgtga ccataatcga
    17941 gcaacgcatc agtgacttta cgcatcatgc ggctttcagg agcaaagcgg tgccagtaga
    18001 gcatccgtcg ttgaaatagc cctggcgtta agtcaatcag ttcaccgctg gccagctctt
    18061 tctcgatttg caggtgcggg atcatacagc aggtggtgcc ctgacgagca agttgcacga
    18121 acgcttccga agaattaacg atatggcagg gtacgctgcc aggaggcaga tcgaagtttt
    18181 gctgcaaaaa ggcctggtgc atatcgtcaa gatggtcaaa agcgaccaca ggtgctttca
    18241 ataatgccga acgcgttacg ccgttaggga aatatttttc ggcaaacggt tttgagctga
    18301 cgaacagata gtcgagcgca ccgagcttat cgacaaggca actcggcagc gcctgatgtt
    18361 gaatactcac cgcgccgacc acttcgccgc ggcgcagacg ttcctgagtg cgggtttcat
    18421 cttctacctg caagttgagg cggataggcg aatcagctaa cacaggagcc agcgccggaa
    18481 gcaaccacgt cgccagactg tcagcgttga ccgccagtga aagcagcagc ggagttgaac
    18541 cggtttgttc atcgcccagc cactcttctt ccagtaactc cacctggcgt agcagtgcca
    18601 gcagtttttg cccttgttct gtcgggcgcg gtggtacggt acgcaccaac agcggctgcc
    18661 cgaacatatt ttccagttgc ttaatgcgct gtgagacggc tgattgtgta atgcacagct
    18721 tttgtgccgc gcgctcaaat cctcgttcac gtatcaccgc atccagtgcc tgtaatgttc
    18781 tgtagtccgg gcgtttcatt gctgttgtct gctcctggta aatgcttttt cctgcactat
    18841 gacacaattt tgtgtcaggt tgcatatacc gaagtcgttg gcgggtaaca tttgtatcag
    18901 tggttaacgc gcagcttaaa agatcaggga tttgcgctaa tgcatttcgc tcagcgagtt
    18961 cgcgccctgg ttgtgttgaa tggcgtggcg ttattgcctc tgttcgcatc gtggagcggc
    19021 atacccagac cgttgtatgc tttatttgcg gggcgaaagg ggatgcccgc cattgcgcga
    19081 tattttatgg atgagttaac cacccggctt gccaacgggg tctgaatcgc tttttttgta
    19141 tataatgcgt gtgaaatttc ataccacagg cgaaacgatc atgacgcagg atgaattgaa
    19201 aaaagcagta ggatgggcgg cacttcagta tgttcagccc ggcaccattg ttggtgtagg
    19261 tacaggttcc accgccgcac actttattga cgcgctcggc acgatgaaag gccagattga
    19321 aggggccgtt tccagttcag atgcttccac tgaaaaactg aaaagcctcg gcattcacgt
    19381 ttttgatctc aacgaagtcg acagccttgg catctacgtt gatggcgcag atgaaatcaa
    19441 cggccacatg caaatgatca aaggcggcgg cgcggcgctg acccgtgaaa aaatcattgc
    19501 ttcggttgcg gaaaaattta tctgtattgc cgacgcctcc aagcaggttg atattctggg
    19561 caaattcccg ctgccagtag aagttatccc gatggcacgt agtgcagtgg cgcgccagct
    19621 ggtgaaactg ggcggtcgtc cggaataccg tcagggcgtg gtgaccgata acggcaacgt
    19681 gatcctcgac gtccacggca tggaaatcct tgacccgata gcgatggaaa acgccataaa
    19741 tgcgattcct ggcgtggtga ctgttggctt gtttgctaac cgtggcgcgg acgttgcgct
    19801 gattggcaca cctgacggtg tcaaaaccat tgtgaaatga tctgacgggg gaacctcccc
    19861 cgttaaaaaa attctcttca ttaaatttgg tgacatgtgt cacgttttta cctggctatt
    19921 gtcgattgct ctaaataaac cctctaaacc agcatattca tccaagaaat acctttgcgt
    19981 gatatttcct caatatcgtg acgcaaacgt tcatattgcc gcaatattat tttttgatat
    20041 gttgaaaggc ggatgcaaat ccgcacacaa catttcaaaa gacaggattg ggtaaatggc
    20101 aaaggtatcg ctggagaaag acaagattaa gtttctgctg gtagaaggcg tgcaccaaaa
    20161 ggcgctggaa agccttcgtg cagcgggtta caccaacatc gaatttcaca aaggcgcgct
    20221 ggatgatgaa caattaaaag aatccatccg cgatgcccac ttcatcggcc tgcgatcccg
    20281 tacccatctg gctgaagacg tgatcaacgc cgcagaaaaa ctggtcgcta ttggctgttt
    20341 ctgtatcgga acaaaccagg ttgatctgga tgcggcggca aaacgcggga tcccggtatt
    20401 taacgcaccg ttctcaaata cgcgctctgt tgcggagctg gtgattggcg aactgctgct
    20461 gctattgcgc ggcgtgccgg aagccaatgc taaagcgcat cgtggcgtgt ggaacaaact
    20521 ggcggcgggt tcttttgaag cgcgcggcaa aaagctgggt atcatcggct acggtcatat
    20581 tggtacgcaa ttgggcattc tggctgaatc gctgggaatg tatgtttact tttatgatat
    20641 tgaaaacaaa ctgccgctgg gcaacgccac tcaggtacag catctttctg acctgctgaa
    20701 tatgagcgat gtggtgagtc tgcatgtacc agagaatccg tccaccaaaa atatgatggg
    20761 cgcgaaagag atttcgctaa tgaaacccgg ctcgctgctg attaatgctt cgcgcggtac
    20821 tgtggtggat attccagcgc tgtgtgacgc gctggcgagc aaacatctgg caggggcggc
    20881 aatcgacgta ttcccgacgg aaccggcgac caatagcgat ccatttacct ctccgctgtg
    20941 tgaattcgac aatgtccttc tgacgccaca cattggtggt tcgactcagg aagcgcagga
    21001 gaatatcggt ctggaagttg cgggtaaatt gatcaagtat tctgataatg gttcaacgct
    21061 ctctgcggtg aacttcccgg aagtctcgct gccactgcac ggtggtcgtc gtctgatgca
    21121 catccacgaa aaccgtccgg gtgtgctgac tgcgctcaac aaaatttttg ccgagcaggg
    21181 cgtcaacatc gccgcgcaat atctgcaaac ttccgcccag atgggttatg tagttattga
    21241 tattgaagcc gacgaagacg ttgccgaaaa agcgctgcag gcaatgaaag ctattccggg
    21301 taccattcgc gcccgtctgc tgtactaaat ccccttatct gaaaatcaac gggcaggtca
    21361 ctgacttgcc cgttttttta tcccttctcc acaccgttgc cgcattgcgc attctacttt
    21421 gcgagtctgc tccagaaaat aaatcaaagt cagatcaatg cgttgtgttt atttaatggg
    21481 gatttacgat ggcagggcag catggggctg taacggtaaa gccctcaccg aagcgagggc
    21541 ttgaaggaga agggttatga tgcgacttgt catcatactg attgtactgt tactcataag
    21601 tttcccagct tattaacagt cagtctcagg ggaggagtca tcctccctta cccttactca
    21661 ctaaattagg ttaaagaatc aacgatgtca atcagggcga tgcaggtgtg tcgccctcac
    21721 cactcccaga ctttcgacgg tgtaaccacc gcaggaagag ggatatccca ctcttcaacg
    21781 gggagttttt ccaccaactg acaatcatgc gcataaccca ccggttgcat tttatagtgc
    21841 tgccagtttt gtaaggtccg atcataaaaa ccaccgccca ttcccaggcg ctgaccgtac
    21901 tcatcaaagg cgaccagcgg tgtgatcaac acgtctaatc gggaaagggg tagcacgtca
    21961 cgcacatcca gtttgggctc atggatcttc aacctgttca tcaccagttc gctttgcgga
    22021 tggtaattca ggaacagcaa attaccggca ctgaagggat gcaaaactgg aagatatacg
    22081 cgcttaccgg cgcgccagag ttgttctatg agtggcaggg tgtcgagttc gccatcaaaa
    22141 gagaggaata cagcgaccgt atgtgccatc accaccgggg gataagtcat catccgggta
    22201 gcggcttgtt gacccatttc ctgctgttgt tccggcgtta acgcacgacg acgttgccga
    22261 atcattttgc ggatttcttg tcgggataat gtcagtggga gttctggtag ttgcgtcatg
    22321 gctggtgcca gataagaagg gaatctccga gatgccgccg caggctgtaa cccttgaacc
    22381 cttggttcaa ggtgaatgtg tcgtcgcagt tttaaggctt ctcggacgga ccgagcatgc
    22441 tcaccaaccg cggagcgcca cattcttgtg gtatgaaata tcggctcagg ggactggccc
    22501 gcttgcgaac atctcagaga aattttgtct tcacggttac tctaccacag taaaccgaaa
    22561 agtgtcattc aaagttttgg ttagtttttt cggtgatgcg accttgttca aataacgctt
    22621 gttctatggt ctgctgcagc atccgaatac gctgttccat acttgccgca tagtcacgag
    22681 tcttcgcttt ttcttgcgct aactcatagc tgatattcaa tgcggcaatg aagaccaact
    22741 gttcagtatt tgtgactcta gtgcgttctt tcagatcttg caaccgttgg ttcagatcgt
    22801 ccgctgcctg attcaacgca tccctttggt caggcgggca gttcacacgc agtgaacggc
    22861 caaaaatttg gatatcgacg ggttgtgcag acatgccacc ttcctgctga ttgactgcgc
    22921 tgccttcgtc tttagaccca tggtctgcga aggggcgaca ctatagctac cctgatgaga
    22981 agagacaagc ccttttctgg tccaccaggg cccaaagtgg tagcatatca tgaatattcc
    23041 tccctttgac gacgaatgct tatgtctata cagaacgaaa tgcctggtta caacgaaatg
    23101 aaccagtatc tgaaccaaca agggacgggt ctgacccctg ctgagatgca tggtttaatc
    23161 agcgggatga tatgtggcgg taacgatgac agctcatggc tgccgctact tcacgacctg
    23221 acgaacgaag gcatggcttt cggtcatgag ctggcacagg cactgcgtaa aatgcactct
    23281 gccaccagcg atgccctgca ggatgacggc ttcctttttc agctttatct gcctgatggc
    23341 gatgatgtca gcgttttcga tcgggctgat gcgctggcag gttgggtcaa tcacttcctg
    23401 cttggtcttg gcgttacgca accgaagctg gacaaagtga ccggagaaac cggtgaagcc
    23461 atcgacgatc tgcgtaacat cgcgcagctg ggctacgacg aagacgaaga tcaggaagag
    23521 cttgaaatgt cgcttgaaga gatcatcgaa tacgttcgtg ttgccgcgct gttatgccac
    23581 gacaccttta ctcatccgca accgaccgcg ccagaagtac aaaaaccgac tctacactaa
    23641 aaacaaaaaa cgtaaggaga gtgttatgag tgagatatcc cggcaagagt ttcagcgtcg
    23701 ccgtcaggcc ctggtggagc aaatgcaacc cggcagcgcc gcgctgattt ttgctgcacc
    23761 agaagtaaca cgtagcgccg acagcgaata cccctatcgt cagaacagtg acttctggta
    23821 cttcaccggc tttaacgaac cggaagcggt gctggtgctg attaaaagcg acgacactca
    23881 taaccacagc gttttgttta accgcgttcg cgacctgacg gcagagatct ggtttggtcg
    23941 tcgcttaggc caggatgccg cgccagagaa actgggcgtt gaccgcgcac tggcattcag
    24001 cgaaatcaat cagcaacttt atcaactgct taatggtctg gatgtggttt atcacgctca
    24061 gggcgaatat acctatgctg atgaaatcgt gaacagcgcg ctggaaaagc tgcgtaaagg
    24121 ctcgcgacag aatctcactg ccccggctac gatgatcgac tggcgtcctg ttgttcatga
    24181 aatgcgcctg ttcaaatcac cagaagagat tgccgtactt cgccgcgcgg gtgaaatcac
    24241 cgcactggcg catacccggg cgatggaaaa atgccgtccg gggatgttcg agtaccatct
    24301 ggaaggcgaa attcaccacg agtttaaccg ccacggtgcg cgttaccctt cctataacac
    24361 cattgttggc agcggtgaaa acggctgcat tttgcactac accgaaaacg agtgtgaact
    24421 gcgtgacggt gatctggtgt tgattgacgc gggttgtgaa tacaaaggtt acgctggcga
    24481 cattacccgc accttcccgg tcaacggcaa attcactcag gctcagcgtg aaatctatga
    24541 cattgtgctg gagtctcttg aaaccagcct gcgcctgtat cgtccgggaa cttccatgca
    24601 ggaagtcact ggtgaagtgg tgcgtatcat ggttagcggc ctggtaaaac tcggcatcct
    24661 gaagggtgat gttgatgaac tgatcgctca gaacgcccat cgtcctttct ttatgcatgg
    24721 ccttagccac tggttaggac tggatgtcca tgacgttggc gtttatggtc aggatcgctc
    24781 gcgcattctg gaaccgggga tggtactgac cgtagagcct ggtctgtata tcgcgccgga
    24841 tgcggatgtg ccagaacaat atcgcggtat cggcattcgt attgaagacg acattgtgat
    24901 taccgaaacc ggcaacgaaa acctcaccgc cagcgtggtg aaaaagccgg aagaaatcga
    24961 agcgttgatg gctgctgcga gaaagcaatg agcgtaatca tcgtcggtgg cggcatggcg
    25021 ggcgcgacgc tggcgctggc tatttcccgg ttaagtcacg gggcgctgcc ggtacatttg
    25081 attgaagcga ctgcgccaga gtcacatgct catccgggct ttgatggacg agcgatagcg
    25141 ctggcggcgg gtacctgtca gcaactggcg cgcatcggcg tctggcaatc tctggcggat
    25201 tgcgcaaccg ccatcactac cgtgcatgtc agcgatcgtg gtcatgccgg attcgtcacc
    25261 ctcgccgcag aagattacca actggcggcg ctggggcagg ttgtcgaatt gcataatgtc
    25321 gggcaacggc tgtttgcatt gctgcgcaaa gcacctggcg taacgctgca ttgtcctgat
    25381 cgcgtggcta acgttgcccg tactcagagt cacgttgaag tgacgctgga gagtggcgag
    25441 acgctgacgg gctgcgtgct ggtagccgct gatggcaccc attcagcgtt agccaccgtg
    25501 tgcggcgttg actggcagca ggagccttac gaacaactgg ctgtgattgc caacgttgcg
    25561 acttccgttg cgcatgaagg gcgcgctttt gaacgcttta cacaatatgg cccgctggcg
    25621 atgttgccga tgtctgacgg acgctgttcg ctggtctggt gtcatccact ggaacggcgc
    25681 gaagaggtac tgtcttggag cgacgagaag ttttgccgtg aactccagtc ggcctttggt
    25741 tggcgacttg ggaaaattac ccacgctggt aaacgcagtg cttatccgct ggcgttaacc
    25801 cgcgccgcca gacctattac ccatcgtacc gtgctggtgg gcaatgcggc gcaaactctg
    25861 cacccgattg ccgggcaagg gttcaacctc ggtatgcgag atgtcatgag ccttgcggaa
    25921 accctgactc aggcgcagga gcgcggagaa gacatgggtg attacggcgt attgtgccgt
    25981 tatcagcagc gtcgacagcg cgatcgcgaa gcaaccatcg gcgtcacgga cagccttgta
    26041 catctttttg ccaaccgttg ggggccgctg gttgtcgggc gcaacatcgg gctgatggca
    26101 atggaattat tcaccccagc acgcgatgtg ctggcgcagc gcaccctcgg gtgggtggcg
    26161 cgttgaaggc ggttttaagg aataaacaat gcaaagtgtt gatgtagcca ttgttggcgg
    26221 cggcatggtg gggctggcgg ttgcctgcgg cttgcagggt agcggcttac gcgttgccgt
    26281 actggagcag cgcgtaccgg aacctctggc ggcggatgca ccaccacaac tgcgcgtttc
    26341 ggctatcaat gccgccagcg aaaaattact cacccgtctt ggtgtctggc aggacattct
    26401 ctctcgtagg gccagttgtt atcacggtat ggaagtgtgg gacaaagaca gttttggtca
    26461 catttcgttt gacgatcaaa gcatgggcta tagccatctt gggcatatcg ttgaaaactc
    26521 ggtgattcac tacgcgctgt ggaacaaagc gcagcagtcg tcagatatca ctctgttggc
    26581 ccccgcagaa ttacagcagg tcgcctgggg agaaaatgaa accttcctga cgttgaaaga
    26641 tggcagtatg ttaacggcgc gtctggtgat tggcgcggac ggcgctaatt cctggttgcg
    26701 caacaaagcc gatattccgc tgactttctg ggattatcag catcacgcgc tggtagcgac
    26761 cattcgcacg gaagaaccgc atgatgcggt ggcgcggcag gtttttcatg gcgaaggcat
    26821 tctggccttt ttgccgctta gcgatccgca tctttgctcg attgtctggt cactgtcgcc
    26881 agaggaagcg cagcggatgc agcaggcagg cgaagacgaa tttaatcgcg cgttaaatat
    26941 cgcttttgat aatcgcctgg gcttatgcaa ggttgaaagt gagcgcctgg tgttcccgct
    27001 aacggggcgt tatgcgcgcc agtttgccgc acaccgtctg gcgctggtgg gcgacgccgc
    27061 gcatactatt cacccgctgg cggggcaggg ggtaaatctc ggctttatgg atgctgcaga
    27121 gctgattgct gaactgaaac ggttgcatcg tcagggtaaa gacatcgggc agtacattta
    27181 cctgcgtcgc tatgagcgta gccgtaagca cagtgcggcg ctgatgctgg ctggtatgca
    27241 gggattccgc gatctgtttt ccggtaccaa tccggcgaaa aaattgctgc gtgatattgg
    27301 tctgaaactg gctgatacgc ttcctggcgt taagccacaa cttattcgtc aggcaatggg
    27361 attaaacgat ttgcctgaat ggctgcgtta aatatttctc ttctgctgtt tatttgatac
    27421 ccatcacact ttcatctccc ggttttttcg ccgggagatt ttcctcattt gaaataaact
    27481 aatttaacct ccgttttcgc attatatttt ctaatgttat ggatttttga tttagtgttt
    27541 tttgacatt
//
LOCUS       DAZKGF010000054        27543 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZKGF010000054 DAZKGF010000000
VERSION     DAZKGF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.054"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            287..502
                     /gene="essD"
                     /locus_tag="ACHOQV_002152"
     CDS             287..502
                     /gene="essD"
                     /locus_tag="ACHOQV_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFP9630524.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            502..999
                     /gene="rrrD"
                     /locus_tag="ACHOQV_002153"
     CDS             502..999
                     /gene="rrrD"
                     /locus_tag="ACHOQV_002153"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP9630525.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            1216..1398
                     /gene="rzoD"
                     /locus_tag="ACHOQV_002154"
     CDS             1216..1398
                     /gene="rzoD"
                     /locus_tag="ACHOQV_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFP9630526.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1489..1782)
                     /gene="iss"
                     /locus_tag="ACHOQV_002155"
     CDS             complement(1489..1782)
                     /gene="iss"
                     /locus_tag="ACHOQV_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFP9630527.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(2143..2337)
                     /gene="ylcI"
                     /locus_tag="ACHOQV_002156"
     CDS             complement(2143..2337)
                     /gene="ylcI"
                     /locus_tag="ACHOQV_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9630528.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            2727..3272
                     /gene="nohD"
                     /locus_tag="ACHOQV_002157"
     CDS             2727..3272
                     /gene="nohD"
                     /locus_tag="ACHOQV_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFP9630529.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            3247..5172
                     /locus_tag="ACHOQV_002158"
     CDS             3247..5172
                     /locus_tag="ACHOQV_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9630530.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            5169..5375
                     /locus_tag="ACHOQV_002159"
     CDS             5169..5375
                     /locus_tag="ACHOQV_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFP9630531.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            5372..6973
                     /locus_tag="ACHOQV_002160"
     CDS             5372..6973
                     /locus_tag="ACHOQV_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9630532.1"
                     /translation="MKTSSIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            6954..8273
                     /locus_tag="ACHOQV_002161"
     CDS             6954..8273
                     /locus_tag="ACHOQV_002161"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9630533.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            8283..8615
                     /locus_tag="ACHOQV_002162"
     CDS             8283..8615
                     /locus_tag="ACHOQV_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9630534.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGTAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            8671..9696
                     /locus_tag="ACHOQV_002163"
     CDS             8671..9696
                     /locus_tag="ACHOQV_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9630535.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            9738..10133
                     /locus_tag="ACHOQV_002164"
     CDS             9738..10133
                     /locus_tag="ACHOQV_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP9630536.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            10145..10498
                     /locus_tag="ACHOQV_002165"
     CDS             10145..10498
                     /locus_tag="ACHOQV_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9630537.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            10510..11088
                     /locus_tag="ACHOQV_002166"
     CDS             10510..11088
                     /locus_tag="ACHOQV_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9630538.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRISGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            11085..11480
                     /locus_tag="ACHOQV_002167"
     CDS             11085..11480
                     /locus_tag="ACHOQV_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9630539.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            11488..12228
                     /locus_tag="ACHOQV_002168"
     CDS             11488..12228
                     /locus_tag="ACHOQV_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9630540.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTAS"
     gene            12244..12666
                     /locus_tag="ACHOQV_002169"
     CDS             12244..12666
                     /locus_tag="ACHOQV_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9630541.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            12648..13082
                     /locus_tag="ACHOQV_002170"
     CDS             12648..13082
                     /locus_tag="ACHOQV_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9630542.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            13075..15654
                     /locus_tag="ACHOQV_002171"
     CDS             13075..15654
                     /locus_tag="ACHOQV_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9630543.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMAD
                     SRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSG
                     GGR"
     gene            15651..15980
                     /locus_tag="ACHOQV_002172"
     CDS             15651..15980
                     /locus_tag="ACHOQV_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9630544.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            15980..16678
                     /locus_tag="ACHOQV_002173"
     CDS             15980..16678
                     /locus_tag="ACHOQV_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9630545.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            16684..17427
                     /locus_tag="ACHOQV_002174"
     CDS             16684..17427
                     /locus_tag="ACHOQV_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9630546.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSETDRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLSGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            17424..17996
                     /locus_tag="ACHOQV_002175"
     CDS             17424..17996
                     /locus_tag="ACHOQV_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9630547.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            18056..21553
                     /locus_tag="ACHOQV_002176"
     CDS             18056..21553
                     /locus_tag="ACHOQV_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9630548.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITW
                     TITSANIDRLRVTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPIWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKRLTAPTITSGGNP
                     PAFSLTSDGRLTAKNADISGSVNANSGTLNNVTINQNCTIKGMLEATQVRGDFVKAVS
                     KAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            21624..22223
                     /locus_tag="ACHOQV_002177"
     CDS             21624..22223
                     /locus_tag="ACHOQV_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HFP9630549.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            22288..25314
                     /locus_tag="ACHOQV_002178"
     CDS             22288..25314
                     /locus_tag="ACHOQV_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9630550.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSAKAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLASGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTTYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYVLMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNIGAHTHSVSGTAASAGNHTH
                     SVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHT
                     HSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            25311..26213
                     /locus_tag="ACHOQV_002179"
     CDS             25311..26213
                     /locus_tag="ACHOQV_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HFP9630551.1"
                     /translation="MITLILSAPVTEMAEAFKRVFANADNVNIVGKPFETIREFDCMV
                     SAANSFGLMDGGVDAAITTFFGSQLQARVQNHILREYLGEQPVGSAFVIETGHNYHPW
                     LVHAPTMRVPLTIDGTDAVYNATWAALLAIFQHNKNATTDRKIKTVVFPAMGAGCGQV
                     PFESVARQMKQAWDNFNKKTESINWEYAQSRQSAVFGTYAYCPGNSVCRYADTKYIGC
                     GDYRTYCSRSGQVCINPEHQADDVLIQHQANNRFRPGSHIHRINPENPVGNVISGAHS
                     HGSSIVIGAHTHTLNKQYSVSDIK"
     gene            26222..26797
                     /locus_tag="ACHOQV_002180"
     CDS             26222..26797
                     /locus_tag="ACHOQV_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9630552.1"
                     /translation="MDFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPALP"
     gene            complement(26895..27485)
                     /locus_tag="ACHOQV_002181"
     CDS             complement(26895..27485)
                     /locus_tag="ACHOQV_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9630553.1"
                     /translation="MSRIYAYCRVSTLEKTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT         6583 a         6855 c         8216 g         5889 t
ORIGIN      
        1 atttgcttat gtatttgtag tggtgttttc aatactcggt agcattctcg caaatatcat
       61 tttgtggttt acgtacgtaa aaaattggtt atgctgttaa gagtggttac ttcgtcacac
      121 agcttaaacc cgccgtcgag cgggtttttc cattttttga gtctcgatat tagctgataa
      181 cccaatacct gagttattca ctgactccga gtctgttacg tttctgcttt tttgcgatac
      241 gttgtattcc ctcaatttac acccgctttg tctgcgaggt ggggttatga aatccatgga
      301 taagttaaca acgggtgtcg cctatggcac ctcagcaggt agtgccggtt actggttttt
      361 acagctgctc gataaagtca cgccctcaca gtgggcagca ataggtgtgc tgggtagcct
      421 ggtatttggc ctgctgacgt acctgacaaa cctttatttc aagattaaag aagataagcg
      481 caaggctgcg agaggtgaat aatgcctcca tcattacgaa aagccgttgc tgctgctatt
      541 ggtggcggag caattgctat agcatcagtg ttaatcactg gcccaagtgg taacgatggt
      601 ctggaaggtg tcagctacat accatacaaa gatattgttg gtgtatggac tgtatgtcac
      661 gggcatacag gaaaagacat catgctcggt aaaacgtata ccaaagcaga atgcaaagcc
      721 ctcctgaata aagaccttgc cacggtcgcc agacaaatta acccgtacat caaagtcgat
      781 ataccggaaa caatgcgcgg cgctctttac tcattcgttt acaacgtggg tgctggcaat
      841 ttcagaacat cgacgcttct tcgcaaaata aaccagggcg atatcaaagg cgcatgtgat
      901 cagctacgtc gctggacata tgctggcggt aagcaatgga aaggtctcat gactcgtcgt
      961 gagattgagc gtgaaatctg tttgtggggt cagcaatgaa cagagtaacc gcgattatct
     1021 ccgctctggt tatctgcatc atcgtctgcc tgtcatgggc tgttaatcat taccgtgata
     1081 acgccattac ctacaaagcc cagcgcgaca aaaatgccag agaactgaag ctggcgaacg
     1141 cggcaattac tgacatgcag atgcgtcagc gtgatgttgc tgcgctcgat gcaaaataca
     1201 cgaaggagtt agctgatgcg aaagctgaaa atgatgctct gcgtgatgat gttgccgctg
     1261 gtcgtcgtcg gttgcacatc aaagcagtct gtcagtcagt gcgtgaagcc accaccgcct
     1321 ccggcgtgga taatgcagcc tccccccgac tggcagacac cgctgaacgg gattatttca
     1381 ccctcagaga gaggctgatc actatgcaaa aacaactgga aggaacccag aagtatatta
     1441 atgagcagtg cagatagagc tgcccatatc gatgggcaac tcatgcaatt attgtgagca
     1501 atacacacgc gcttccagcg gagtataaat gcctaaagta ataaaaccga gaaatccatt
     1561 tacgaatgtt tgctgggttt ctgttttaac aacattttct gcgccgccac aaattttggc
     1621 tgcatcaaca gttttctcct gtccaattcc cgaaacgaag aaatgatggg tgatggtttc
     1681 ctttggtgtt actgctgtcg gtttgtttcc aacagtaaac gtctgttgag cacatcctgt
     1741 aataagcatt gccagagcgg cagaaaacaa catttttttc atcttattat cctgcattgt
     1801 taaaaacggc agaatcctat gtgacaacaa ttaaacgata gttaaatgga ttgatgaaaa
     1861 ttaaaactat ataggtggat gctcagccta ttggaggagg gggggcactc agaatcctgt
     1921 ggaatgaaat aaaccgctct atctgtccat taccctttta gctgcgctgt atcgtcgccg
     1981 tattcccgca ttaaccatga ccgtagcccg acggggaatt ccttctgcgt gagtgtgcgg
     2041 gaataatcaa aaacgatgca caccgggttt tactgtgctg acagacgcag ggttaccctc
     2101 atagtcgctt ttccggtgcg atggtggaag aaaccgggat gttcatccat catcactttg
     2161 gattgatgta tatgctctct tttctgacgt tagtctccga cggcaggctt caatgaccca
     2221 ggctgagaaa ttcccagacc ctttttgctc aagagcgatg ttaatttgtt caatcatttg
     2281 gttaggaaag cggatgttgc gggttgttgt tctgcgggtt ctgttcttcg ttgacatgag
     2341 gttgccccgt attcagtgtc gctgatttgt attgtctgaa gttgttttta cgttaagttg
     2401 atgcagatca attaatacga tacctgcgtc ataattgatt atttgacgtg gtttgatggc
     2461 gtagatgcac gttgtgacat gtagatgata attattatca tttttgcggg tcctttccgg
     2521 cgatccgaca ggttacgggg cggcgacctc gcgggttttc gctatttatg aaaattttcc
     2581 ggtttaaggc gtttccgttc ttcttcgtca taacttaatg tttttattta aaataccctc
     2641 tgaaaagaaa ggaaacgaca ggtgctgaaa gcgagctttt tggcctctgt cgtttccttt
     2701 ctctgttttt gtccgtggaa tgaacaatgg aagtcaacaa aaagcagctg gctgacattt
     2761 tcggtgcgag tatccgtacc attcagaact ggcaggaaca gggaatgccc gttctgcgag
     2821 gcggtggcaa gggtaatgag gtgctttatg actctgccgc cgtcataaaa tggtatgccg
     2881 aaagggatgc tgaaattgag aacgaaaagc tgcgccggga agttgaagaa ctgcggcagg
     2941 ccagcgagac agatctccag ccagggacta ttgagtacga acgccatcga cttacgcgtg
     3001 cgcaggccga cgcacaggag ctgaaaaatg ccagagactc cgctgaagtg gtggaaaccg
     3061 cattctgtac tttcgtgctg tcgcggatcg caggtgaaat tgccagtatt ctcgacggga
     3121 tccccctgtc ggtgcagcgg cgttttccgg aactggaaaa ccgacatgtt gatttcctga
     3181 aacgggatat catcaaagcc atgaacaaag cagccgcgct ggatgaactg ataccggggt
     3241 tgctgagtga atatatcgaa cagtcaggtt aacaggctgc ggcattttgt ccgcgccggg
     3301 cttcgctcac tgttcaggcc ggagccacag accgccgttg aatgggcgga tgccaattac
     3361 tatctcccaa aagaatccgc ataccaggaa gggcgctggg aaacactgcc ctttcagcgg
     3421 gccatcatga atgcgatggg cagcgactac atccgtgagg tgaatgtggt gaagtctgcc
     3481 cgtgtcggtt attccaaaat gctgctgggt gtttatgcct actttataga gcataagcag
     3541 cgcaacaccc ttatctggtt gccgacggat ggtgatgccg agaactttat gaaaacccac
     3601 gttgagccga ccatccgcga tattccgtcg ctgctggcgc tggctccgtg gtatggcaaa
     3661 aagcaccggg ataacacgct cactatgaag cgtttttcca atggtcgtgg cttctggtgc
     3721 ctgggcggta aagcggcaaa aaactaccgt gaaaagtcgg tggatgtggc gggttatgat
     3781 gaacttgctg cctttgatga ggatattgaa caggaaggct ctccgacgtt ccttggcgac
     3841 aaacgtattg aaggctcggt ctggccaaag tccatccgtg gctccacgcc caaagtgaga
     3901 ggcacctgcc agattgagcg tgcagccagt gaatccccgc attttatgcg ttttcatgtt
     3961 gcctgcccgc actgcgggga ggagcagtat cttaaatttg gcgacaaaga gacgccgttt
     4021 ggcctcaaat ggacgccgga tgacccctcc agcgtgtttt atctctgcga gcataatgcc
     4081 tgcgtcatcc gccagcagga gctggacttt actgatgccc gttatatctg cgaaaagacc
     4141 gggatctgga cccgtgatgg cattctctgg ttttcgtcat ccggtgaaga gattgagccg
     4201 ccggacagtg tgacctttca catctggacg gcgtacagcc cgttcaccac ctgggtgcag
     4261 attgtcaaag actggatgaa gacgaaaggg gatacgggaa aacgtaaaac cttcgtgaac
     4321 accacgctcg gtgagacatg ggaagcgaaa attggcgaac gtccggatgc tgaggtgatg
     4381 gcggagcgga aagagcatta ttcagcgccc gttcctgacc gtgtggctta cctgaccgcc
     4441 ggtatcgact cccagctgga ccgctacgaa atgcgcgtat ggggatgggg gccgggtgag
     4501 gaaagctggc tgattgaccg gcagattatt atgggccgcc acgacgatga acagacgctg
     4561 ctgcgtgtgg atgaggccat caataaaacc tatacccgcc ggaatggtgc agaaatgtcg
     4621 gtatcccgta tctgctggga tactggcggg attgacccga ccattgtgta tgaacgctcg
     4681 aaaaagcatg ggctgttccg ggtgatcccc attaaagggg catccgtcta cggaaagcca
     4741 gtggccagca tgccacgtaa gcgaaacaaa aacggggttt accttaccga aatcggtacg
     4801 gataccgcga aagagcagat ttataaccgc ttcacactga cgccggaagg ggatgaaccg
     4861 cttcccggtg ccgttcactt cccgaataac ccggatattt ttgatctgac cgaagcgcag
     4921 cagctgactg ctgaagagca ggtcgaaaaa tgggtggatg gcaggaaaaa aatactgtgg
     4981 gacagcaaaa agcgacgcaa tgaggcgctc gactgcttcg tttatgcgct ggcggcgctg
     5041 cgcatcagta tttcccgctg gcagctggat ctcagtgcac tgctggcgag cctgcaggaa
     5101 gaggatggtg cagcaaccaa caagaaaaca ctggcagatt acgcccgtgc cttatccgga
     5161 gaggatgaat gacgcgacag gaagaacttg ccgctgcccg tgcggcactg catgacctga
     5221 tgacaggaaa acgggtggca acggtacaga aagacggacg gcgagtggag tttacggcca
     5281 cttccgtgtc tgacctgaaa aaatacattg cggagctgga agtgcagacc ggcatgacac
     5341 agcgacgcag gggacctgca ggattttatg tatgaaaacg tcctccattc ccacccttct
     5401 ggggccggac ggcatgacat cgctgcgtga atatgccggt tatcacggcg gtggcagcgg
     5461 atttggtggg cagttgcggg cgtggaaccc accgagtgaa agtgtggatg cagccctgtt
     5521 gcccaacttt acccgtggca atgcccgcgc ggacgatctg gtacgcaata acggctatgc
     5581 cgccaacgcc atccagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca
     5641 tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga
     5701 ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg
     5761 aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga
     5821 actgttcgtt caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg
     5881 gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg
     5941 tgccggtgtg cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg
     6001 gtatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg
     6061 tgcctcgttc attcacgttt ttgaacccgt ggaggacggg cagacccgcg gtgcaaatgt
     6121 gttttacagc gtgatggagc agatgaagat gctcgacacg ctgcagaaca cgcagctgca
     6181 gagcgccatt gtgaaggcga tgtatgccgc caccattgag agtgagctgg atacgcagtc
     6241 agcgatggat tttattctgg gcgcgaacag tcaggagcag cgggaaaggc tgaccggctg
     6301 gattggtgaa attgccgcgt attacgccgc agcaccggtc cgtctgggag gcgcaaaagt
     6361 gccgcacctg atgccggggg actcactgaa cctgcagacg gctcaggaca cggataacgg
     6421 ctactccgtg tttgaacagt cactgttgcg gtatatcgct gccgggctgg gtgtctcgta
     6481 tgagcagctt tcccggaatt acgcccagat gagctactcc acggcacggg ccagtgcgaa
     6541 cgagtcgtgg gcgtacttta tggggcggcg aaaattcgtc gcatcccgtc aggcgagcca
     6601 gatgtttctg tgctggctgg aagaggccat cgttcgccgc gtggtgacgt taccttcaaa
     6661 agcgcgtttc agctttcagg aagcccgcag cgcctggggg aactgtgact ggataggctc
     6721 cggtcgtatg gccatcgatg gtctgaaaga agtacaggaa gcggtgatgc tgatagaagc
     6781 cggactgagt acctacgaga aagagtgcgc aaaacgcggt gacgactatc aggaaatttt
     6841 tgcccagcag gtccgtgaaa cgatggagcg ccgtgcagcc ggtcttaaac cgcccgcctg
     6901 ggcggctgcg gcatttgaat ccggactgcg acaatcaaca gaggaggaga agagtgacag
     6961 cagagctgcg taatctcccg catattgcca gcatggcttt taatgagccg ctgatgcttg
     7021 aacccgccta tgcgcgggtt ttcttttgtg cgcttgcagg ccagcttggg atcagtcgcc
     7081 tgacggatgc agtatccggc gacagcctga ctgccggaga ggcacccgcg gcgctggcgt
     7141 tatccggtga tgatgacgga ccacgacagg cccgcagtta tcaggtcatg aacggcatcg
     7201 ccgtgctgcc ggtgtccggt acgctggtca gccggacgcg ggcgctgcag ccgtattcgg
     7261 gaatgaccgg ttacaacggc attatcgccc gtctgcaaca ggctgccagc gatccgatgg
     7321 tggacggcat tctgctcgat atggacacac cgggcgggat ggtggcggga gcatttgact
     7381 gtgctgacat catcgcccgt gtgcgtgaca taaagccggt atgggcgctg gccaacgaca
     7441 tgaactgcag tgcaggtcag ctgcttgcca gcgccgcctc ccggcgtctg gtcacgcaga
     7501 ccgcccggac aggctccatc ggcgtcatga tggctcacag taattacggc gctgcgctgg
     7561 agaaacaggg cgtggaaatc acgctgattt acagcggcag ccataaggtg gatggcaacc
     7621 cctacagcca tctaccgggt gatgtccggg agacactgca gtcccggatg gatgcaaccc
     7681 gccggatgtt tgcgcagaag gtgtcggcat ataccggcct gtccgtgcag gctgtgctgg
     7741 ataccgaggc tgcagtgtac agcggtcagg aggccattga tgccggactg gctgatgaac
     7801 ttgtcaacag caccgatgcg atcaccgtta tgcgtgatgc actggatgca cgtaaatccc
     7861 gtctctcagg agggcgaatg accaaagaga ctcaatcaac aactgtttca gccactgctt
     7921 cgcaggctga cgttactgac gtggtgcaag cgacggaggg cgaaaacgcc agcgcggcgc
     7981 agccggacgt gaacgcgcag atcaccgctg cggttgcggc agaaaacagc cgcattatgg
     8041 ggatcctcaa ctgtgaggaa gctcacggac gcgaagaaca ggcacgtgtg ctggccgaaa
     8101 cccccggtat gaccgtggaa acggcccgcc gcattctggc agctgcacca cagagtgcac
     8161 aggcgcgcag tgacactgcg ctggatcgtc tgatgcaggg ggcaccggca ccactggctg
     8221 caggtaaccc ggcatctgat gccgttaacg atttgctgaa cacaccagtg taagggatgt
     8281 ttatgacgag caaagaaacc tttacccatt accagccgct gggcaacagt gacccggcac
     8341 atacggcaac cgcgcccggc ggattgagtg cgaaagcgcc tgcaatgacc ccgctgatgc
     8401 tggacacctc cacccgtaag ctggttgcgt gggatggcac caccgacggt actgccgttg
     8461 gcattctggc ggttgatgct gaccagacca gcaccacgct gacgttctac aagtccggca
     8521 cgttccgtta tgaggatgtg ctctggccgg aggctgccag cgacgagacg aaaaaacgga
     8581 ccgcgtttgc cggaacggca atcagcatcg tttaacctta cccttcatca ctaaaggccg
     8641 cctgtgcggc tttttttacg ggattttttt atgtcgatgt acacaaccgc ccagctgctg
     8701 gcggcaaatg agcagaaatt taagtttgat ccgctgtttc tgcgtctctt tttccgtgag
     8761 agctatccct tcaccacgga gaaagtctat ctctcacaaa ttccgggact ggtaaacatg
     8821 gcgctgtacg tttcgccgat tgtttccggt gaggttatcc gttcccgtgg cggctccacc
     8881 tctgaattta cgccgggata tgtcaaaccc aagcatgagg tgaatccgca gatgaccctg
     8941 cgtcgcctgc cggatgaaga tccgcagaat ctggcggacc cggcttaccg ccgccgtcgc
     9001 atcatcatgc agaacatgcg agacgaagag ctggccattg ctcaggtcga agagatgcag
     9061 gcagtttctg ctgtgcttaa gggcaaatac accatgaccg gtgaagcctt cgatccggtt
     9121 gaggtggata tgggccgcag tgcggcgaac aacatcacac agtccggtgg tacggagtgg
     9181 agcaagcgtg acaagtccac gtatgacccg accgacgata tcgaagccta tgcgctgaac
     9241 gccagcggcg tggtgaatat catcgtgttt gatccgaaag gctgggcgct gttccgttcc
     9301 ttcaaagccg tcaaggagaa gctggatacc cgtcgcggct ctcattccga gctggagaca
     9361 gcggtaaaag acctgggcga agcggtgtcc tataagggga tgtatggcga tacggcgatc
     9421 gtcgtgtatt ccggacagta cgtggaaaac gacgtcaaaa agaacttcct gccggacaac
     9481 acgatggtgc tggggaacac tcaggcacgc ggtctgcgca cctatggctg cattcaggat
     9541 gcggacgcac agcgcgaagg tattaacgcc tctgcccgct acccgaaaaa ctgggtgacc
     9601 accggcgatc cggcgcgtga gttcaccatg attcagtcag caccgctgat gctgctggct
     9661 gatcctgatg cgttcgtgtc cgtacaactg gcgtaatcat ggcccttcgg ggccattttc
     9721 tctctgtgga ggagtccatg acgaaagatg aactgattgc ccgtcttcag gtgctgggtg
     9781 agcaactgaa ccgtgatgtc agcctgacgg ggacgaaaga agaactggtg ctccgtgtgg
     9841 cagagctgga agaggagctt gatgacacgg atgacgctgc cggtcaggac acatctgtca
     9901 gcccggaaaa tgcgctgacc ggacatgaaa atgaggtggt atcagcgcag ccggataccg
     9961 tgattgatac ggctgctctg gtcacggtcg tggcactggt gacgctgcat actgatgcac
    10021 ttcacgccac gcgggatgag cctgtggcat ttgtgctgcc gggaacggcg tttcgtgtct
    10081 ctgccggtgt ggcagccgaa atgacagaac atggcctggc cagaatgcaa taacgggagg
    10141 cgctgtggct gattccgata acctgttcga tgctgccatt gcccgcgccg atgaaacgat
    10201 acgcgggtac atgggaacgt cagccaccat gacatccggt gagctgtccg gtgctgtgat
    10261 acgtggtgtt tttgatgacc ctgaaaatat cagctatgcc ggacaggggg tgcgcgttga
    10321 aggctccagc ccgtccctgt ttgtccggac tgatgatgtg cggcagctgc ggcgtggaga
    10381 cacactgacc atcggcgagg aaaacttctg ggtggaccgg atttcgccgg atgatggcgg
    10441 aagctgtcat ctctggcttg ggcgtggcgt gccgcctgcc gttaaccgtc gccgctgaaa
    10501 gggggatgta tggccataaa aggtcttgag caggccgttg aaaacctcag ccgtatcagc
    10561 aaaacggcgg tgcctggtgc cgccgcaatg gccattaacc gcgttgcgtc atccgcgata
    10621 tcgcagtctg cgtcacaggt tgcccgtgag acaaaggtac gccggaaact ggtaaaggaa
    10681 agggccaggc tgaaaagggc cacggtcaaa aatccgcagg ccagaatcag ggttaaccgg
    10741 ggggatttgc ccgtaatcaa gctgggtaac gcgcgggttg tcctttcccg ccgcagacgt
    10801 cgtaaaaagg ggcagcgttc atccctgaaa ggtggcggca gcgtgcttgt ggtgggaaac
    10861 cgtcgtattt ccggcgcgtt tattcagcaa ctgaaaaatg gccggtggca tgtcatgcag
    10921 cgtgtggccg ggaaaaaccg ttaccccatt gatgtggtga aaatcccgat ggcggtgccg
    10981 ctgaccacgg cgtttaaaca gaatattgaa cggatacggc gtgaacgtct tccgaaagag
    11041 ctgggctatg cgctgcagca tcaactgaga atggtaataa agcgatgaaa catactgaac
    11101 tccgtgcagc cgtactggat gcactggaga agcatgacac cggggcgacg ttttttgatg
    11161 gtcgccccgc tgtttttgat gaggcggatt ttccggcagt tgccgtttat ctcaccggcg
    11221 ctgaatacac gggcgaagag ctggacagcg atacatggca ggcggagctg catattgaag
    11281 ttttcctgcc tgctcaggtg ccggattcag agctggatgc gtggatggag tcccggattt
    11341 atccggtgat gagtgatatc ccggcactgt cagatttgat caccagtatg gtggccagcg
    11401 gctatgacta ccggcgcgac gatgatgcgg gcctgtggag ttcagccgat ctgacttatg
    11461 tcattaccta tgaaatgtga ggacgctatg cctgtaccaa atcctgtaat gccggtgaaa
    11521 ggtgccggga ccaccctgtg ggtttataag ggaagcggtg acccttatgc gaatccgctt
    11581 tcagacgttg actggtcgcg tctggcaaaa gttaaagacc tgacgcccgg cgaactgacc
    11641 gctgagtcct atgacgacag ctatctcgat gatgaagatg cagactggac tgcgaccggg
    11701 caggggcaga aatctgccgg agataccagc ttcacgctgg cgtggatgcc cggagagcag
    11761 gggcagcagg cgctgctggc gtggtttaat gaaggggata cccgagccta taaaatccgc
    11821 ttcccgaacg gcacggtcga tgtgttccgc ggctgggtca gcagtatcgg taaggcggtg
    11881 acggcgaagg aagtgatcac ccgcacggtg aaagtcacca acgtgggccg tccgtcgatg
    11941 gcagaagatc gcagcacggt gacggcgaca accggcatga ctgtgacgcc tgccagcacc
    12001 tcggtggtga aagggcagag caccacgctg accgtggcat tccagccgga aggcgcaacc
    12061 gacaagagct tccgtgcggt gtctgcggat aaaacaaaag ccaccgtgtc ggtcagtggt
    12121 atgaccatca ccgtgaaagg tgttgctgca ggcaaggtca acattccggt cgtatccggt
    12181 aatggtgaac ttgctgcggt tgcagaaatc accgtcaccg ccagttaatc cggggagtca
    12241 gcgatgttcc tgaaaaccga atcatttgaa cataacggtg tgaccgtcac gctttctgaa
    12301 ctgtcagccc tgcagcgaat tgagcatctc gccctgatga aacggcaggc agaacaggcg
    12361 gagtcagaca gcaaccggaa gtttactgtg gaagacgcca tcagaaccgg cgcgtttctg
    12421 gtggcgatgt ccctgtggca taaccatccg cagaagacgc agatgccgtc catgaatgaa
    12481 gccgttaaac agattgagca ggaagtgctt accacctggc ccacggaggc aatttctcat
    12541 gctgaaaacg tggtgtaccg gctgtctggt atgtatgagt ttgttgtgaa taatgcccct
    12601 gaacagacag aggacgccgg gcccgcagag cctgtttctg cgggaaagtg ttcgacggtg
    12661 agctgagttt tgccctgaaa ctggcgcgag agatggggcg acccgactgg cgtgccatgc
    12721 ttgccgggat gtcatccacg gagtatgccg actggcaccg cttttacagt acccattatt
    12781 ttcatgatgt tctgctggat atgcactttt ccgggctgac gtacaccgtg ctcagcctgt
    12841 ttttcagcga tccggatatg catccgctgg atttcagtct gctgaaccgg cgtgaggctg
    12901 acgaagagcc tgaagatgat gtgctgatgc agaaagcggc agggcttgcc ggaggcgtcc
    12961 gctttggccc ggacgggaat gaagttatcc ccgcttcccc ggatgtggcg gacatgacgg
    13021 aggatgacgt aatgctgatg acagtatcag aagggatcgc aggaggagtc cggtatggct
    13081 gaaccggtag gcgatctggt cgttgattta agtctggatg cggccagatt tgacgagcag
    13141 atggccagag tcaggcgtca tttttccggt acggaaactg atgcgaaaaa aacagcggca
    13201 gtcgttgaac agtcgctgag ccgacaggcg ctggctgcac agaaagcggg gatttccgtc
    13261 gggcagtata aagccgccat gcgtatgctg cctgcacagt tcaccgacgt ggccacgcag
    13321 cttgcaggcg ggcaaagccc gtggctgatc ctgctgcaac aggggggtca ggttaaggac
    13381 tccttcggcg ggatgatccc catgttcagg gggcttgccg gtgcgatcac cctgccgatg
    13441 gtgggggcca cctcgctggc ggtggcgacc ggtgcgctgg cgtatgcctg gtatcagggc
    13501 aactcaaccc tgtccgattt caacaaaacg ctggtccttt ccggcaatca ggcgggactg
    13561 acggcagatc gtatgctggt cctgtccaga gccgggcagg cggcagggct gacgtttaac
    13621 cagaccagcg agtcactgac ggcgctggtg aatgccggtg tgcgtggtgg tgagcagttt
    13681 gaggcgatca gccagagtgt ggcgcgtttc tcctctgcat ccggcgtgga ggtggacaag
    13741 gtcgctgaag ccttcgggaa gctgaccaca gacccgacgt cgggactgac agcgatggca
    13801 cgtcagttcc ataacgtgac ggcggagcag attgcgtatg ttgctcagtt gcagcgttcc
    13861 ggagatgaag ccggggcatt gcaggcggcg aacgaggccg caacgaaagg gtttgatgac
    13921 cagacccgcc gcctgaaaga gaacatgggc acgctggaga cctgggcaga caggacagca
    13981 cgggcattca aatccatgtg ggattcggtg ctggatattg gtcgcccgga cactgcccag
    14041 ggaatgctgg agaaagcaga aaaggctttt gatgaggcgg acaaaaaatg gcagtggtat
    14101 cagagccgga gccaccggcg cggtaaaacc tcagcatttc ttgccaatct ccggggagca
    14161 tgggaggaca gagcgaatgc gcaacttggg ctttcagccg ccacgttgca ggccgatctt
    14221 gaaaaggcca gagagatggc agcaaaggac tgggccgagt ctgaggcatc acggctgaaa
    14281 tataccgaag aggcgcagaa ggcttacgaa cggctgcaga cgccgctgga gaaatatacc
    14341 gcccgtcagg aagaactgaa caaggcactg aaagacggga aaatcctgca ggcggattac
    14401 aacacgctga tggcggcggc gaaaaaggat tatgaagcga cgctgaaaaa gccgaaacag
    14461 tccggcgtga aggtgtctgc gggcgatcgt caggaagaca gtgctcatgc tgccctgctg
    14521 acgcttcagg ctgaactccg gacgctggag aagcatgccg gagcaaatga gaaaatcagc
    14581 cagcagcgcc gggatttgtg gaaggcggag agccagttcg cggtactgga ggaggcggcg
    14641 caacgtcgcc agctgtctgc acaggagaaa tccctgctgg cgcataaaga tgagacgctg
    14701 gagtacaaac gccagctggc tgcacttggc gacaaggtta cgtatcagga gcgcctgaac
    14761 gcgctggcgc agcaggcgga taaattcgca cagcagcaac gggcaaaacg ggccgccatt
    14821 gatgcgaaaa gccgggggct gactgaccgg caggcagaac gggaagccac ggaacagcgc
    14881 ctgaaggaac agtatggcga taatccgctg gcgctgaata acgtcatgtc agagcagaaa
    14941 aagacctggg cggctgaaga ccagcttcgc gggagctgga tggcaggcct gaagtccggc
    15001 tggagtgagt gggaagagag cgccacggac agtatgtcgc aggtaaaaag tgcagccacg
    15061 cagacctttg atggtattgc acagaatatg gcggcgatgc tgaccggcag tgagcagaac
    15121 tggcgcagct tcacccgttc cgtgctgtcc atgatgacag aaattctgct taagcaggca
    15181 atggtgggga ttgtcgggag tatcggcagc gccattggcg gggctgttgg tggcggcgca
    15241 tccgcgtcag gcggtacagc cattcaggcc gctgcggcga aattccattt tgcaaccgga
    15301 ggatttacgg gaaccggcgg caaatatgag ccagcgggga ttgttcaccg tggtgaattt
    15361 gtcttcacga aggaggcaac cagccggatt ggcgtgggga atctttaccg gctgatgcgc
    15421 ggctatgcca ccggcggtta tgtcggtaca ccgggcagca tggcagacag ccggtcgcag
    15481 gcgtccggga cgtttgagca gaataaccat gtggtgatta acaacgacgg cacgaacggt
    15541 cagataggtc cggctgctct gaaggcggtg tatgacatgg cccgcaaggg tgcccgtgat
    15601 gaaattcaga cacagatgcg tgatggtggc ctgttctccg gaggtggacg atgaagacct
    15661 tccgctggaa agtgaaaccc ggtatggatg tggcttcggt cccttctgta agaaaggtgc
    15721 gctttggtga tggctattct cagcgagcgc ctgccgggct gaatgccaac ctgaaaacgt
    15781 acagcgtgac gctttctgtc ccccgtgagg aggccacggt actggagtcg tttctggaag
    15841 agcacggggg ctggaaatcc tttctgtgga cgccgcctta tgagtggcgg cagataaagg
    15901 tgacctgcgc aaaatggtcg tcgcgggtca gtatgttgcg tgttgagttc agcgcagagt
    15961 ttgaacaggt ggtgaactga tgcaggatat ccggcaggaa acactgaatg aatgcacccg
    16021 tgcggagcag tcggccagcg tggtgctctg ggaaatcgac ctgacagagg tcggtggaga
    16081 acgttatttt ttctgtaatg agcagaacga aaaaggtgag ccggtcacct ggcaggggcg
    16141 acagtatcag ccgtatccca ttcaggggag cggttttgaa ctgaatggca aaggcaccag
    16201 tacgcgcccc acgctgacgg tttctaacct gtacggtatg gtcaccggga tggcggaaga
    16261 tatgcagagt ctggtcggcg gaacggtggt ccggcgtaag gtttacgccc gttttctgga
    16321 tgcggtgaac ttcgtcaacg gaaacagtga cgccgatccg gagcaggagg tgatcagccg
    16381 ctggcgcatc gagcagtgca gcgaactgag cgcggtcagt gcctcttttg tactgtccac
    16441 gccgacggaa acggacggtg ccgtttttcc gggacgtatc atgctggcca acacctgcac
    16501 ctggacctat cgcggtgatg agtgcggtta tcacggtccg gcggtcgcgg atgaatatga
    16561 tcagccgacg tccgatatca cgaaggataa atgcagcaaa tgcctgagcg gctgtaagtt
    16621 tcgcaataac gtcggcaact ttggcggctt cctttccatt aacaaacttt cgcagtgaat
    16681 cccatgacag agacagaatc agcgattctg gcgcacgccc ggcgatgtgc gccagcggag
    16741 tcgtgcggct tcgtggtgag aacaccggag ggggaaagat attttccctg cgtgaatatc
    16801 tccggtgagc cggaggcgta tttccggatg tcgccggagg actggctgcg ggcagagatg
    16861 cagggtgaga ttgtggcgct ggtccacagc caccccggtg gtctgccctg gctgagtgag
    16921 accgaccggc ggctgcaggt gcagagtgat ttgccgtggt ggctggtttg ccggggggcg
    16981 attcacaagt tccgctgtgt gccacatctt tccgggcggc gctttgagca cggggtgacg
    17041 gactgttaca cgctgttccg ggatgcttac catctggcgg ggattgagat gccggatttt
    17101 catcgcgagg atgactggtg gcgtcacggt cagaatctct atctggataa tctggaggcc
    17161 acagggctgt atcaggtgcc gttgtcatca gcacaaccgg gcgatgtgct gctgtgctgt
    17221 tttggttcat cggtgccgaa tcatgccgcc atttactgtg gtgacagcga gctgctgcac
    17281 catattcctg aacaactgag caaacgagag aggtacaccg acaaatggca gcgacgcaca
    17341 cactccctct ggcgtcaccg ggaatggcac gcatctgcct ttacggggat ttgcaacgat
    17401 ttggccgccg catcgacctt cgtgtgaaaa cgggggctga agccatccgc gcactggcca
    17461 cacagctccc ggcgtttcgt cagaaactga gcgacggctg gtatcaggta cggattgccg
    17521 ggcgggacgt cagcacgtcc gggttaacgg cgcagttaca tgagactctg cctgacggcg
    17581 ctgtgattca tattgttccc agagtcgccg gggccaagtc agggggcgta ttccagattg
    17641 ttctgggggc tgccgccatt gccggatcat tctttactgc cggagccacc cttgcagcat
    17701 ggggggcagc cattggggcc ggtggtatga ccggcatcct gttttctctc ggtgccagta
    17761 tggtgctcgg tggtgtggcg cagatgctgg caccgaaagc cagaactccc cgtacacaga
    17821 caacggataa cggtaagcag aacacctatt tctcctcact ggataacatg gttgcccagg
    17881 gcaatgttct gcctgttctg tacggggaaa tgcgcgtggg gtctcgcgtg gtttctcagg
    17941 agatcagcac ggcagacgaa ggggatggtg gtcaggttgt ggtgattggt cgctgatgaa
    18001 aaacgtttat gtgaaaccgc ctccgggcgg ttttgtcgtt tatggagcgt gaggaatggg
    18061 taaaggcagc agtaaggggc atactccgcg cgaagcgaag gacaacctga agtccacgca
    18121 gttgctgagt gtgatcgatg ccatcagcga agggccgatt gaaggtccgg tggatggatt
    18181 aaaaagcgtg ctgctgaaca gtacgccagt gctggacagt gaggggaata ctaacatctc
    18241 cggcgtcacg gtggtgttcc gggccggtga gcaggagcag acaccgccgg agggatttga
    18301 atcctccggc tccgagacgg tgctgggtac ggaagtgaaa tacgacacgc cgatcacctg
    18361 gaccatcacg tcggcaaaca ttgaccgact gcgtgttacc ttcggcgtgc aggcactggt
    18421 ggaaaccacc tcaaaggggg acaggaatcc gtcggaagtc cgcctgctgg ttcagataca
    18481 gcgtaatggt ggctgggtga cggaaaaaga catcaccatt aagggcaaaa ccacctcgca
    18541 gtatctggcc tcggtggtgg tgggtaacct gccgccgcgc ccgttcagta tacggatgcg
    18601 caggatgacg ccggacagca ccacagacca gctgcagaac aaaacgctct ggtcgtcata
    18661 caccgaaatc atcgatgtga aacagtgcta cccgaacacg gcactggtcg gtgtgcaggt
    18721 ggactcggag cagttcggca gccagcaggt gagccgtaat tatcatctgc gcgggcgtat
    18781 tctgcaggtg ccgtcgaact ataacccgca gacgcggcaa tacagcggta tctgggacgg
    18841 aacgtttaaa ccggcataca gcaacaacat ggcctggtgt ctgtgggata tgctgaccca
    18901 tccgcgctac ggcatgggga aacgtcttgg tgcggcggat gtggataaat gggcgctgta
    18961 tgtcatcggc cagaattgcg accagtcggt gccggacggc tttggcggca cggagccgcg
    19021 catcacctgt aatgcctggc tgaccacaca gcgcaaggcg tgggatgtgc tcagtgattt
    19081 ctgctcggcg atgcgctgta tgccgatatg gaacgggcag acgctgacgt tcgtgcagga
    19141 ccgaccatca gataaggtgt ggacctataa ccgcagtaat gtggtgatgc cggatgatgg
    19201 cgcgccgttc cgctacagct tcagcgccct gaaggaccgc cataatgccg ttgaggtgaa
    19261 ctggattgac ccggataacg gctgggagac ggcgacagag cttgtggagg acacgcaggc
    19321 cattgcccgt tacggtcgta acgtcacgaa gatggatgcc tttggctgta ccagtcgggg
    19381 gcaggcgcac cgcgccgggc tgtggctgat taaaacggaa ctgctggaaa cgcagaccgt
    19441 ggacttcagc gtgggtgcgg aagggcttcg ccatgtaccg ggggatgtca ttgaaatctg
    19501 cgatgatgac tatgcgggta tcagcaccgg cgggcgcgtg ctggcggtga acagccagac
    19561 ccggacgctg acgctcgacc gtgaaatcac gctgccatcc tccggtacca cgctgataag
    19621 cctggttgac ggaagtggca atccggtcag cgtggaggtc cagtccgtca ccgacggcgt
    19681 gaaggtaaaa gtgagccgtg ttcctgacgg cgttgccgga tacagcgtgt gggggctgaa
    19741 gctgccgacg ctgcgccagc gactgttccg ctgcgtgagt atccgtgaga acgacgacgg
    19801 cacgtatgcc atcaccgccg tgcagcatgt gccggaaaaa gaggccatcg tggataacgg
    19861 ggcgcacttt gacggcgacc agagcggcac ggtgaatggt gtcacgccgc cagcggtgca
    19921 gcacctgact gccgaagtca ccgcagacag cggggaatat caggtgctgg cgcgctggga
    19981 cacaccgaag gtggtgaagg gcgtgagctt cctgctccgt ctgaccgtaa cagcggacga
    20041 cggcagtgag cggctggtca gcacggcccg gacgacggaa accacatacc gcttcacaca
    20101 actggcgttg gggaactaca ggctgacagt ccgggcggta aatgcgtggg gacagcaggg
    20161 cgatccggca tcggtatcgt tccggattgc cgcaccggca gcgccgtctc ggattgagct
    20221 gacaccgggc tattttcaga taaccgccac gccgcatctt gcggtttatg acccgacggt
    20281 acagtttgag ttctggttct cggaaaaacg gattgcggat atcaggcagg ttgaaaccag
    20341 cgcgcgttat cttggtacgg cactgtactg gatagccgcc agtatcaata tcaaaccggg
    20401 ccatgattat tatttttacg ttcgcagtgt gaacaccgtt ggcaaatcgg cattcgtgga
    20461 ggctgtcggt cagccgagtg atgatgcatc aggctatctg gattttttca aaggcgagat
    20521 agggaaaacc catctggctc aggagctgtg gacgcagatt gataacggtc agcttgcgcc
    20581 tgacctgact gaaatcagga cgtccataac ggatgtcagc aatgaaataa cacagaccgt
    20641 caataagaaa ctggaagacc agagtgcagc gatccagcag atacagaagg ttcaggttga
    20701 tacaaataat aacctgaaca gcatgtgggc agtgaagctg cagcagatgc aggacggacg
    20761 cctttatatt gcgggtatcg gtgccggtat tgagaacacc cctgacggca tgcagagtca
    20821 ggtgctgctg gcagcagaca ggattgcgat gattaatcct gcgaatggca acacaaagcc
    20881 gatgtttgtt ggtcagggcg atcagatatt catgaatgaa gtgttcctga aacgcctgac
    20941 ggcccccacc attaccagcg gcggtaatcc tccggcattt tccctgacat cagacgggag
    21001 actgacggcg aaaaatgcgg atatcagtgg cagtgtgaat gcgaactcag gaacgctcaa
    21061 caatgtcacg attaaccaga actgtacgat taagggcatg ctggaggcga cccaggtcag
    21121 aggagatttc gttaaagctg tatcaaaagc cttcccgaaa aaagtcggta cgtggggtaa
    21181 cacggaaaca ccaaacggta cggttacagt caccatcagc gatgatcata actttgaccg
    21241 ccagattatt attccgccca ttatttttaa cggtatagcg tatgacgatc cggggagcgg
    21301 aaataaccca ggaggcacgc gatacacggg ttatggtttt gaagttcgca aaaacggcgt
    21361 attaatcgca tccagagaaa ctaaaggggc cattcccggt agttacagtg cagttattga
    21421 tatgccgagt ggcaggggaa gcgtcactct ggagtttaag attttccaga aaggcaatca
    21481 gggggcaggc aatatcaccg actgtacggt gattgtgacc aaaaaagccg cttccggcat
    21541 cagtattcgt tgaaatattt ataaccccaa taaagggcgt caggaatgac gcctttttta
    21601 ttgcagaaaa gcgagaggta attatgcgta aagtttgtgc agcaattttg tccgcagcca
    21661 tttgtctggc cgtatccggt gcgcctgcat gggcgtctga acatcagtcc acgctgagcg
    21721 cggggtatct tcatgcctcg acgaacgtcc ccggcagcga tgatctgaac gggattaacg
    21781 tgaaataccg ttatgagttt acggacacac tggggctggt gacgtcattc agctatgcag
    21841 gagacaagaa tcgccagctg acccgttaca gcgatacccg ctggcatgaa gattccgttc
    21901 gtaaccgctg gttcagcgta atggcggggc cgtctgtgcg cgtgaatgaa tggttcagcg
    21961 cgtatgcgat ggcgggtgta gcttacagcc gtgtgtcgac tttctccggg gattatcttc
    22021 gcgtaactga caacaagggg aaaaagcacg atgtgctgac cggaagtgat gacggtcgcc
    22081 acagcaacac gtctctggcg tggggggctg gcgtgcagtt taacccgacc gaatccgtgg
    22141 ccattgatat tgcttatgaa ggctccggca gtggtgactg gcgcactgac ggtttcatcg
    22201 tgggtgtcgg ttataaattc tgattagcca ggtaacacag tgttatgaca gcccgccggt
    22261 tcaggcgggc ttttttgtgg ggtgaatatg gcagtaaaga tttcaggtgt actgaaagac
    22321 ggcacaggaa aaccggtaca gaactgcaca atccagctga aagcaaaacg taacagcacc
    22381 acggtggtgg tgaacacgct ggcctcagaa aatccggatg aagccgggcg ttacagcatg
    22441 gacgttgagt acgggcagta cagcgttatt ctgttggtgg agggattccc gccgtcacat
    22501 gccgggacca tcaccgtgta tgaagattcc cgacccggta cgctgaatga ttttctcggt
    22561 gccatgacgg aggatgatgc ccgtcctgag gcactgcgcc gttttgaact gatggtggaa
    22621 gaggtggcgc gtaacgcgtc cgcggtggca cagaacacgg cagccgcgaa gaagtcagcc
    22681 agcgatgcca gcacatcagc ccgtgaggcg gcaacccatg cgactgatgc tgcagattca
    22741 gcacgtgcag ccagcacgtc agccggacag gccgcttcgt cggctcagtc agcgtcttcc
    22801 agcgcaggaa cggcatcaac aaaggccact gaagcctcaa aaagtgctgc cgctgcagag
    22861 tcctcaaaaa gcgtggcagc taccagtgcc ggtgcggcga aaacgtcaga aacgaatgcc
    22921 gcagcgtcag aacaatcagc cgccacttct gcatccaccg cgaccacgaa agcgtcagaa
    22981 gctgccacct cagcccggga tgcggcggcc tcaaaagagg cagcgaaatc atcagaaacg
    23041 aacgcatcct tgagcgccag tagcgcagct tcctcggcaa cggcggcagg aaattccgcg
    23101 aaggcggcaa aaacgtccga gacgaacgcc aggtcttctg agacggcagc gggacagagc
    23161 gcctcagctg cggcaggctc aaaaacagcg gctgcgtcgt ctgccagtgc cgcgtcaaca
    23221 agtgccgggc aggcctcagc cagtgccacc gccgccggaa aatcggcaga aagcgccgca
    23281 tcatccgctt caacagccac aacgaaggct ggcgaagcca ctgagcaggc cacggcagca
    23341 gcgaggtcag cttccgcagc gaagacatcc gaaacgaacg cgaaagcgtc ggaaacccgt
    23401 gcagaatcct caaaaacggc agcggcatcg tccgccagtt cggcggcgtc atcggcatca
    23461 tcggcgtctg cttcaaaaga tgaggcgacc agacaggcgt cagcagcgaa gggcagcgcc
    23521 acgacggcat ccacgaaggc gacagaggct gctggtagtg cgaaggcagc agctcagagc
    23581 aaaagtacgg cggaatctgc agcaacgcgc gctgagacag cggcaaaacg ggcagaggat
    23641 attgcatccg ccgtggcgct tgaggatgcg agcacgacga aaaaggggat agtacagctc
    23701 agtagtgcga ctaacagcac ttccgagtca ctggcggcaa caccaaaagc cgttaaggcc
    23761 gcgtatgacc tggctagcgg gaaatacacc gcacaggatg caacgacagc acagaaaggg
    23821 ataatccagc taagcagcgc gaccaacagc acgtctgaaa cgctggcggc aacgccaaag
    23881 gcagtaaaag cagccaatga caatgctgag aaacgtctgc agaaagatca gaacggtgcg
    23941 gatatccctg gcaaagacac ctttacgaaa aatattggtg cctgccgtgc cttcggtggg
    24001 tcagtaagca caacaacagg aaactggacg actgcacagt ttatcgagtg gctggattct
    24061 cagggagcat ttaaccatcc atactggatg tgcaagggtt cctggtctta tggcaataat
    24121 aaaatcatta ctgatactgg ctgcggtaat attcatctcg ccggagctgt cattgaagta
    24181 atggggataa agtcagcgat gacgatccgc attaccactc cgaccacatc cagcggtggc
    24241 ggaacaacca acgcccagtt tacctatatt aatcacggaa cagattattc acctggctgg
    24301 cgaagggact ataactccag aaataagcca acggcatcag agatcggggc gctaccgtca
    24361 ggtggaacag cagtatcatc agttaatctg gcttcaaaag gtcgggtagc cgcgctgaca
    24421 gacaatacgc agggggcagc aggtcttgag ttatacgagg tgtataacaa cggatatcca
    24481 acaacgtatg gaaatatcat tcacctgaaa gggatgacag ccgttggcga aggtgagtta
    24541 ctcatcggct ggagtggtac aagcggtgct catgctccgg catttattcg ttcacgacgg
    24601 gatacgaccg acgcaaactg gtcgccgtgg gcgcagcttt acacctcggc tcatcctcct
    24661 gcagagtttt atccagtcgg tgcaccaatc ccgtggccat cagataccgt tccgtctggt
    24721 tatgtcctga tgcaggggca gacttttgac aaatctgcat acccgaaact tgcagccgct
    24781 tatccgtcag gcgtgatccc tgatatgcgt ggctggacga ttaagggcaa acctgccagt
    24841 ggtcgggccg tattgtctca ggaacaggac ggcattaaat cgcacaccca cagcgccagc
    24901 gcatccagta cggatttggg gacgaaaacc acatcgtcgt ttgattacgg cactaaatcc
    24961 acgaataaca tcggggcgca tacgcacagt gtgagcggta cagccgcaag tgccggaaac
    25021 catactcata gtgtcacagg cgcatcagca gtcagccagt ggtcacaaaa tgggtcagta
    25081 cataaggtag tgtctgcggc cagtgtgaat acaagtgctg caggagcgca cactcatagt
    25141 gtcagcggca cagctgcatc tgcaggtgct cacgcacata ctgtcggtat tggtgctcat
    25201 acgcactcgg ttgcgattgg ctcacatgga cacaccatca ccgttaacgc tgctggtaac
    25261 gcggaaaaca ccgtcaaaaa catcgcattt aactatattg tgaggcttgc atgattacgc
    25321 tcattctttc tgcaccagta accgaaatgg ctgaagcatt taagcgggta tttgcaaatg
    25381 cagataatgt gaatattgtc ggaaagccat ttgaaacaat cagagaattt gactgcatgg
    25441 taagtgcggc aaatagtttc ggcctgatgg atggcggtgt tgatgccgcc attaccacat
    25501 tcttcggttc tcagctacag gcccgcgttc agaatcatat tcttcgtgaa tatctcggcg
    25561 agcagcctgt aggttctgca tttgttattg aaacggggca taattatcac ccctggctgg
    25621 tacatgcgcc aacaatgcgt gttccgttga caattgacgg aacagacgct gtatataacg
    25681 caacttgggc agctctactt gctatctttc agcacaataa aaacgcaacg acagacagga
    25741 aaataaagac ggtggtattc cctgcaatgg gagccggatg tggtcaggtg ccgtttgaaa
    25801 gcgttgcccg gcagatgaag caggcatggg ataactttaa taaaaaaaca gaatcaatta
    25861 actgggaata cgcacaatcc cgccagtcgg cagtatttgg cacatatgca tactgtccgg
    25921 gtaattctgt ttgccgttat gcggatacta aatatattgg atgcggcgat tatcggacgt
    25981 attgctcacg ttccgggcag gtctgtatta accctgaaca tcaggctgat gatgtactga
    26041 tacaacatca ggctaataac cggtttcgcc ctggttcgca tatacaccgg attaatccag
    26101 aaaatcctgt aggtaatgtc atctctggcg cacatagcca cggaagtagc atcgttattg
    26161 gtgctcacac ccatacgctc aataaacaat attctgtctc tgatattaag tagaggtgaa
    26221 catggatttc agaatgagtg aacaaccacg gaccataaca atttataatc tgctggccgg
    26281 aactaatgaa tttattggtg aaggtgacgc atacattccg cctcatacag gtctgccagc
    26341 aaacagtacc gatattgcac cgccagatat tccggctggt ttcgtggctg ttttcaacag
    26401 tgatgagtca tcgtggcatc tcgttgaaga tcatcggggt aaaacggttt atgacgtggc
    26461 ttccggcgac gcgttattta tttctgaact cggctcatta ccggaaaatg tcacctggtt
    26521 atccccggaa ggggagtttc agaagtggaa cggcacagcc tgggtgaagg atacggaagc
    26581 agaaaaactg ttccggatcc gggaggcgga agaaacaaaa aacagcctga tgcaggtagc
    26641 cagtgagcat atagcgccgc ttcaggatgc tgtggatctg gagatcgcaa cggagaaaga
    26701 aaccttgttg ctggaagcct ggaaaaagta tcgggtgttg ctgaaccgtg ttgatacatc
    26761 aactgcaccg gatattgaat ggccagccct accgtagggt aagatatata aattctataa
    26821 ttagaagtat ctttccattt aaggctagga aggggggctt ggaaaacgta aggaatctca
    26881 caccgagatt atttttatat atcaggtgtc tgattttttg ctttagctct taaaatggtt
    26941 tgccgcgagg ttttgaattc ttgggcaatg tcacttatat ttatacctga cttaatttgc
    27001 tctagtaccc tctgtatttg ttcatcattt aacacaggtg ggcgaccaaa tcgctttcct
    27061 gcgccccgtg ctcttaatat ccctgaatga gtacgctcaa gtaaaaggtc tcgctcaaat
    27121 tcagcgactg ctgaaattac ttgcatcatc atttttccag gtggactggt caggtcaacg
    27181 ccccccaatg ctaagcaatg cactttgata cctgctttgg tcagttgttc caccgtttta
    27241 ctgatatcca ttggattaca accgaggcga tccagttttg tgacaatcaa tgcatcacct
    27301 cttttcagtc gatcaagcaa ctggttaaaa ccaggacgct cactggttgc tgctgagccg
    27361 ctaatttgtt cttcaattat ttgctgagat ttgatgttaa aacctgcact ttcgatttcc
    27421 cgacgttgat ttttggttgt cttttccaga gttgataccc gacagtaagc ataaattcga
    27481 gacatagtga gatcttctat acgaaattgg tgtacatatc ataatgcatc tcagaaaata
    27541 att
//
LOCUS       DAZKGF010000055        27123 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZKGF010000055 DAZKGF010000000
VERSION     DAZKGF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.055"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..1970)
                     /locus_tag="ACHOQV_002182"
                     /pseudo
     CDS             complement(1..1970)
                     /locus_tag="ACHOQV_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(1967..3508)
                     /locus_tag="ACHOQV_002183"
     CDS             complement(1967..3508)
                     /locus_tag="ACHOQV_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA--acetoacetyl-CoA transferase"
                     /protein_id="HFP9630554.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHIMAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKSNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRKVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWMSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDRYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(3518..4294)
                     /locus_tag="ACHOQV_002184"
     CDS             complement(3518..4294)
                     /locus_tag="ACHOQV_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFP9630555.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNEVFNRLRNLPQLTV
                     ANLNGHTVGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQVANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            complement(4304..5146)
                     /locus_tag="ACHOQV_002185"
     CDS             complement(4304..5146)
                     /locus_tag="ACHOQV_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9630556.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNINVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(5156..5947)
                     /locus_tag="ACHOQV_002186"
     CDS             complement(5156..5947)
                     /locus_tag="ACHOQV_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFP9630557.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            6160..6807
                     /locus_tag="ACHOQV_002187"
     CDS             6160..6807
                     /locus_tag="ACHOQV_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9630558.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKNNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(6791..7429)
                     /gene="ytfB"
                     /locus_tag="ACHOQV_002188"
     CDS             complement(6791..7429)
                     /gene="ytfB"
                     /locus_tag="ACHOQV_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFP9630559.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            7647..8267
                     /gene="fklB"
                     /locus_tag="ACHOQV_002189"
     CDS             7647..8267
                     /gene="fklB"
                     /locus_tag="ACHOQV_002189"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9630560.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8576..9988
                     /gene="cycA"
                     /locus_tag="ACHOQV_002190"
                     /gene_synonym="dagA"
     CDS             8576..9988
                     /gene="cycA"
                     /locus_tag="ACHOQV_002190"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFP9630561.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(10033..10695)
                     /gene="ytfE"
                     /locus_tag="ACHOQV_002191"
     CDS             complement(10033..10695)
                     /gene="ytfE"
                     /locus_tag="ACHOQV_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFP9630562.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(10803..11768)
                     /gene="ytfF"
                     /locus_tag="ACHOQV_002192"
     CDS             complement(10803..11768)
                     /gene="ytfF"
                     /locus_tag="ACHOQV_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9630563.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPGFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            complement(11876..12736)
                     /gene="qorB"
                     /locus_tag="ACHOQV_002193"
     CDS             complement(11876..12736)
                     /gene="qorB"
                     /locus_tag="ACHOQV_002193"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP9630564.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADSSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            12825..13205
                     /gene="ytfH"
                     /locus_tag="ACHOQV_002194"
     CDS             12825..13205
                     /gene="ytfH"
                     /locus_tag="ACHOQV_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9630565.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(13334..15277)
                     /gene="cpdB"
                     /locus_tag="ACHOQV_002195"
     CDS             complement(13334..15277)
                     /gene="cpdB"
                     /locus_tag="ACHOQV_002195"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFP9630566.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAVFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            15467..16207
                     /gene="cysQ"
                     /locus_tag="ACHOQV_002196"
     CDS             15467..16207
                     /gene="cysQ"
                     /locus_tag="ACHOQV_002196"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFP9630567.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAVAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(16197..16754)
                     /gene="ytfJ"
                     /locus_tag="ACHOQV_002197"
     CDS             complement(16197..16754)
                     /gene="ytfJ"
                     /locus_tag="ACHOQV_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP9630568.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            17079..17285
                     /gene="ytfK"
                     /locus_tag="ACHOQV_002198"
     CDS             17079..17285
                     /gene="ytfK"
                     /locus_tag="ACHOQV_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFP9630569.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(17347..18690)
                     /gene="paeA"
                     /locus_tag="ACHOQV_002199"
     CDS             complement(17347..18690)
                     /gene="paeA"
                     /locus_tag="ACHOQV_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9630570.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(19013..19651)
                     /gene="msrA"
                     /locus_tag="ACHOQV_002200"
     CDS             complement(19013..19651)
                     /gene="msrA"
                     /locus_tag="ACHOQV_002200"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9630571.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            19857..21590
                     /gene="tamA"
                     /locus_tag="ACHOQV_002201"
     CDS             19857..21590
                     /gene="tamA"
                     /locus_tag="ACHOQV_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFP9630572.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            21587..25366
                     /gene="tamB"
                     /locus_tag="ACHOQV_002202"
     CDS             21587..25366
                     /gene="tamB"
                     /locus_tag="ACHOQV_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFP9630573.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSMPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYAISMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHELPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            25369..25710
                     /gene="ytfP"
                     /locus_tag="ACHOQV_002203"
     CDS             25369..25710
                     /gene="ytfP"
                     /locus_tag="ACHOQV_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFP9630574.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            25877..26200
                     /locus_tag="ACHOQV_002204"
     CDS             25877..26200
                     /locus_tag="ACHOQV_002204"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630575.1"
                     /translation="MFDREMINENKEAFFLYVNGRGELSAQCVIKVAEKGDYLQGFSL
                     TRRAFRTFLKDRVIQMFSSDQELNKVASETDFSQYIAPPENLHPKRIQFQFVLPDLTS
                     LINLA"
     gene            26158..27069
                     /locus_tag="ACHOQV_002205"
     CDS             26158..27069
                     /locus_tag="ACHOQV_002205"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFP9630576.1"
                     /translation="MCFTGFDQSDKSRLSALAKENGIDVKSDVGKSLFFLCVGKNKGW
                     RKVQKASDYGVIILTEEQFINFIDTGEIPLEQDCVVENDISKSLEKDVNTYKSMIDEF
                     SLSIRTVREFRRSNALIAVFVDGYAAGWRFAVKECYKDSLDIKLTPVKTEFFNYEIWT
                     QGDSFQFNRGDVFYSDRLAYDSYCDFLKLDDAVVLQVKYECFSGYDSVSTFDGVMDGE
                     FSFSRMEPRPVHKLPVLIETQLYDPGKIIVDVFVPDAAKKQLKLKDTLELKQDEFVCL
                     LQSGCCWVKRREAGDKKKPEFINLFKK"
BASE COUNT         6575 a         6780 c         7131 g         6637 t
ORIGIN      
        1 aaaagaacct tgcgaatatt gaattgttaa tttgtgattt ttgtcttcag cgagttcaga
       61 gcgaaaacgc cattcttctt ttgcgccgcg acgagtttgt ggtaggcatt tgccttgtgg
      121 atcaataacc atacgcgccc cacggctacc accaccagca tcaatataat gcgttaattg
      181 tgttaatacc gcggcagaag tcagcgccat atgccgccac ataaacagct cggcaacttc
      241 acccacatgt ttgattgcca atccatgccg ttggacaaat tcgttcaata gcagggcatc
      301 acgagtggca cgtcggactt tatcggcatg gcaaataaat cccgcatagt cagacattcg
      361 tgcctgaatt ttttctctca caaccgacaa cggcatcccg gtcccgttat cgtgcgcctg
      421 agtaatgatc tctcttatag aagccagtgc cggagctacc agctgtgcta tatctccatc
      481 aatattacgt ttttgcgtgc aaccaataaa acgtgccaga cgaacagcaa aaacctgccc
      541 ggcattcaat gccgcaccac cagggcgagt gacgccgtgt gtgccagcaa cttcccccac
      601 ggcgaaacag ccgggcaggg aggtttgtcc ccagatatcc acttcaatgc cgccattcat
      661 atgctgatta ttcatggcaa actgcaaagg ctgtgtggtg agatcgtaac cgtgcatctt
      721 atacagcgag atagacagcg gattcattcg ttgcagccgt tcgatgggcg atggtgccag
      781 agcgtcgtta ttttccagat acgcgcgaac gtcgtcgtcc agtcgatcta atgagaaggg
      841 caggtcaccc gcaccgcttc aggattgcga ttgaaatcca taaatacctg acgccctgat
      901 tgctgctctt gcgctacagc catatctaac aagctggagc caaactccat tacccgagtg
      961 gcgtggaacg gccactgata gcctttacga aaaatgtttg aagccagctc ctgggtggtg
     1021 cgatagtaat ccgcgaggaa gttatactcg ttgcccccgg catccacgga atagatatgt
     1081 gggatcacct gcacataggt gccggacaaa ttccacggaa acgtgctgcg cggtgtgcct
     1141 ataccaaact ggctttcagt caaattggtt agcgtcagac cttcttccag cgccagcccc
     1201 agcgagccga aacatttgtg tgggtacaca ctgtcgcgat aaagctcgcc aggccctcct
     1261 gttgccagta ccacattggg cgcaatcaca attgccagcc cccatgggtt atggacgcga
     1321 tgaccggtcg cgaggatagc ccccgccacg cggtcttcgc cgttttcgtc acgctgatgc
     1381 agcagtttaa tcactgttgc actggtcaac attgggatag caagacgctg tacttcttcc
     1441 agcagcactt tcaccattaa ccttgaggta cgtggcccac acgaggttgc acgcccggct
     1501 tcgtcatggt cggtttgata acgaagaatc gcaccatagc gatcttccgg taattccaga
     1561 ccaagatatt gcaacccgcc aagagtgtgc agagaaccta ccgcttcgac ataagcagtg
     1621 tcgtgatcca tcgccccacc actcgccagt gcctctgcca gtttggtgaa gttgtcgccg
     1681 ttgcccgcgg tagcggcggt aaacagcgtc tgtttatcgg aaccggagca ggccgatgtt
     1741 cccatgtata acccggcggt ggcgattaac acattttgct ggcggcgttt aagttccaca
     1801 gctgcacgca gcccggctgc accactgccg actaccaggg catctgtttg ccaaagtggg
     1861 taatcaacac cgtctaccgt gacaacccat gcgggttctg cctgtgcaaa accctttggg
     1921 aaagtaacgt gcgtcagcgc atcaaggatg gtgtcatcaa gctgactcat gcatcctcca
     1981 tcggcgtaaa caggctactt tccatcaggc gtgggtgctt gataattgga cggaaagcca
     2041 tatacggcag aatgtcgcgc tcaatctcca cacccggcgc gatttcaatt aattcaacgc
     2101 cttctgccgt taactggaat actgcgcgtt cagtgatata cagcacttcc tgaccacttt
     2161 gctgtgcgta ggcggcgcta aaagtgattt tttccacttg ggtaaccagt ttagggattt
     2221 gacctgactg ggcgatatgc aggccatcag gcgttacatc aatcttgctg cctttggcgt
     2281 caaacgtacc gcagaacacc actttacggg cgttttgcgc aatttcgagg aagccgccgg
     2341 ggccaatgag attgccattg agatgggata cgttgacgtt accgtaccga tccatctctc
     2401 ccatgccgag gaaggtgata tcgataccgc cgccactgta gaattcgaat tgatcgagtg
     2461 atggaataat ggcgtcggcg ttacgggcgc agccgaacag agcatcatcc agcatcatgc
     2521 cgttatgtac gccttgctcg acgctcatcc ataaactttg ataaggcacg ccttcgcgca
     2581 gcgcaacccc aggaataccg cccggaatac caaagccaaa gttggttgaa gcgcctgcca
     2641 ccagttcccg tgctgcccga cgggcaatca ggcgacgcac cggatgacta accagttcaa
     2701 tagcgtcgtt agaacttaga cggcgatgtt gaccgctgat cgtcaggtcg taaccgccaa
     2761 gataggtttg cggttgttcg cggtgttcaa cgataccatc gaccagaatc cccggtagct
     2821 tgaccttgcg aggttcaatg gcgccagctt ccagtacatc gcgcacctgt acaaacactt
     2881 tgccgccact gttgtgtgct gccagcgcca tcgaatagct atccatatca atcgcttctt
     2941 cttcgaggct aacgttgcca cgcggatcgg cataggtgcc gcgcaaaatc gcgtagtcca
     3001 ctttgaaagg gcgatagcgg agtttggttt caccgtcgat ttcaatcagt tctaccaggt
     3061 cgtcggtcgt gcattcattc gacttaccgc caccattgcg tggatcaaca aacgatccca
     3121 gcccaacgtg ggtaaaaagc cccggacgtc cggcaccgat ctcccgcagc agtgcctgaa
     3181 ttacgccacc agggaaacag taggcttcaa tagcattgct tttcaccagt tcctgcatct
     3241 tgggcgacca ggtaaaatgt ccggcaataa tgcgtttgag catttcggcg tgagcaaaac
     3301 ggttagtgcc tttgctgtcg cgatcgccaa tacccagcga gtggattaat gtcagatcgc
     3361 gcgggtgtcc ggtttgcagg aaacgcgctt caatggcggc cataatatgg tcagcttcca
     3421 ccataccgcc gccgttaccg ctaatggcaa tggttgcgcc gtcctgaatt tgcgccgcca
     3481 gtgtctcggc ggttgataac ttacgcatac aatttcctta ttgattgtgg aagctcacgc
     3541 cggctttttc ggcaaatgcg tgcgtcgcct gctggcaatc ctgagtggcg gacattaatc
     3601 cagccagtaa ctggtgggag taatcggcgt gtttattttc cagcgccaga atcagttgct
     3661 taatatgtgc cagtgccacc ggaccacatt tttccagctg cgctaactgg ttcgccatcc
     3721 agctttccac ctgttctttt tctaccactt catcaattaa attcgccacc tgggcttcct
     3781 gcgccgtcag gcgtttaccc agcatcagca tctggcgacc gaccaccggg ccaacctgat
     3841 taagcacgcg ttcaatgccc atccagccag gcaccatgcc gagcatcact tccgggttag
     3901 agaatttcgc gccggggcga gcgatacgaa tatcgcagca caaggcaagt tcaataccgc
     3961 caccgacggt atgaccgttg agattcgcaa cggttaattg gggtaagttg cgcaggcgat
     4021 taaaaacctc gttaccgcgt ttaatccatt ttctgcccat atctaatggc gaataggccg
     4081 accacgattt aatatcgccg cccgcacaaa agaatttatc gccggttgcc gttaattcga
     4141 ctaatcgtaa tgtggtgtcg ttttcaattt catttaaata atggtcgaga gattcgatca
     4201 tttcttcatt aatagcatga catttatctt cacggttcaa tgtcaggcgg caagaagccg
     4261 ccgctctgct gaacagaacg ggttggtcac tcatgattat tccttagcaa taatgtgcga
     4321 tgcgatcgac gctaatatcc agcgtgctgt taatatcttt ctgccaccag tacggtgaga
     4381 atatttccag ttcaatagcg ccattaaatc cggcgttttc aaccagccga cgaattgacg
     4441 gaatattgat gacaccatct cccggcattc cacggtcatt gaccagatcg gtggtcggca
     4501 ctaaccagtc ggaaacatga aacgcgagga tgcgttttcc ggcacgcagg atctgactgg
     4561 cgagatccgg gtcccaccag acatggtaga catctaccgc cacgccgagg ccaaattcgc
     4621 catcgggatc gagttcatca caccagtcca gcgcctgacg taacgtgcac agacaagaac
     4681 gatcggcagc agtcatcggg tgcagtggct ccagtgcaat tggcacgccg acctctttcg
     4741 aatgtggcag caactggcga atcccttgtt tcacctgttc ccgtgcttcg tacagatctt
     4801 ttgttcccat tggcagaccg ccaaccacct gcatgtaaca ggcggcattt agcacagcgg
     4861 catcgtccag cgcccggcgg ttatcatcaa tcgccagctt gcgctccgcc agcgtcggtg
     4921 cggtgtagta ggtgctacgg cacaaaccgg aaacgtttat gttactggca gcaagctggc
     4981 gggcgatttg ctggagatct tcaccctgca actccctgcg ccagggggca atggcgccaa
     5041 taccgcgtgc ggcgcaggca tcaataatgg cgggtagcgg cgttttatag cccagtgtcg
     5101 cggtattgat actgaataaa tccgggcgtt gtaacagatc tctcatgatt cagcctcaga
     5161 agcgcggcat acttaagccg ccgtcgatgg cgaccgcctg accacaggtg taaatcagtt
     5221 taccttccgc catcgcgcgg acagtggaag caatatcagc cggataaccc caacgtcccc
     5281 acggcaccag acctttggca atcagttcgt cgtaataggc cgttgccgga atagtcatat
     5341 cggttttgat gagaccagga cgcacttcat atacgccaat ttgttcatta caaaggcgag
     5401 cagcaaatag acgagcagcg gcagagacgg cagttttggc aatggtatat tcgccgcgat
     5461 tcatcgccag catgatggcg ttgatggaac tgacgaaaat aatcgagcgg tgcggcagtt
     5521 ctgcttccgg tttcggctgt gccagtaagc gcttactgaa tgcctgagcg aggaagaacg
     5581 gtgcgcgggt gttaatggca atattttggt cgaagctgtc tggttcgagg tcgagaagat
     5641 caccgcgttt tttcactgaa atgcctgcgt tgttgagcag gcagtccaga cgtccccact
     5701 ggttttgcgc tgcgtcgagc atctcttcat gcagggagag atcgcccaca tcagcaggga
     5761 agcaaatcac ttccacgcct tccgccagac attcttgttg tgtggcatgt aatttttcta
     5821 cgctatccgc atccggacga tcattaatca gcagattaaa tccgccacgc gcaagctcca
     5881 gcgcacagcc ctttccaata ccacgagcgg ctccggtaat cacggcaaca ggacgagtgg
     5941 atttcataca atagctctct ttttttgaat tttgtttcct tagtgatgat tagcttgcga
     6001 ctggagggta aaaagatcaa ccatctagtt ggttgatatg cgatggcgat cacaggacat
     6061 ttgataacct gatgaaagta caagtttttg cccttttttc tgaagctgaa atcaaatcag
     6121 gttatgatag tcaccacgaa gaggtcgtgg ggaagcgtta tgtatcttga taccagcaac
     6181 gtacagagcc tgaaagaaaa gctgctgtta tgcgcggtta atgaatttgc cgaatatggt
     6241 tacgaagggg ccagagtcga taatatcgtc aaggccgcgg ggtgcagtaa acagacggtt
     6301 tatcaccact ttggtaataa agaaaattta tttatcgaag tactggaata tacctggaac
     6361 gatattcgac aaaaagaaaa ggcccttgat ttctcagatc ttccgccaca gaaagccatt
     6421 gagaaaataa tcgattttac atgggattat tatatttcta atccctggtt tttaaaaatt
     6481 gtgcatagtg aaaatcaaag taagggtgtg cattatgcaa agtcacagcg tcttttagaa
     6541 attaaccatg cgcacttgca gttaatggaa tccttattgg atgaagggaa gaaaaacaat
     6601 atttttaagc cagatatcga cccattgcag gtgaatatta atattgctgc gcttggggga
     6661 tattatttga tcaaccagca tacgcttggt ctggtttatc acatcagtat ggtttcaccc
     6721 caggcactgg aagccagacg taaggtcatc aaagagacaa tccttagctg gcttttggtt
     6781 gacccttcat ctaccgcgcg cgaataaaac tgccatccgg ctggcgggta aacaggacct
     6841 gttgattatt ccccgtatca atggttaagc ccgtcaccac gccgctggcg ttctggcgga
     6901 ttttcaccat ctgcccgttt tgcaaattac tcagtggctt atccgcgcct tctacctgcg
     6961 ccatggcata gacatcggta gcgggcagtc cgtggtcgcg gaacagctgc gccatcgttt
     7021 taccgggttc gacacgatag gagcgccatt gattatcaat tccgctatct ggctggaatg
     7081 gttgcgtatg ggtggtttgc ggctgctctt ctggctggcc ttcctgtatt ggttctggag
     7141 cgaccggagc cacctgatca ggatcatttt gtggcgtgac taattgcgcc cgcagctgtt
     7201 cttctgtcgg tggttgtgat tgcgactgaa tatctaactg cgcttcacgc gtcaccaccg
     7261 gcgcgttggg cgtatcatca gatggaagca gaaatccgac taccaataca atggcggcaa
     7321 taatgatccc ccgacgatgc atcggcggca gcgggtccat gaagcgaaaa ttgtccggcg
     7381 cgtgccagac tttctccagg gttggtttta gttcaaagcg cccgggcatg gtttcctcct
     7441 gctccgcgtc ttgttcctca atcatagcct atgaataagc taacgctatg atgtccgtgg
     7501 taaacccgcc tttattatat tcatacggga tattgctatt gtttcttttt ccccgggatt
     7561 tgtcatcatt cccgcgacaa agttttaccc aaagaagtgt ggctgatatg ctgcccgcta
     7621 ctttataccc taagaaagga aatacgatga ccaccccaac ttttgacacc atcgaagcgc
     7681 aagcaagcta cggcattggt ttgcaggtag ggcaacaact gagtgaatct ggcctggaag
     7741 ggctgctgcc agaagcactg gttgcaggca ttgccgatgc gctggaaggc aaacatccgg
     7801 ctgttccggt tgatgtggtg caccgcgcgc tgcgtgaaat ccacgaacgc gccgatgccg
     7861 ttcgtcgtca gcgtttccag gcgatggctg ctgaaggtgt gaaatacctg gaagaaaacg
     7921 ccaaaaaaga aggtgtaaat agcaccgaat ctggcctgca attccgcgtg atcaaccagg
     7981 gcgaaggcgc aattccggca cgtaccgacc gcgttcgtgt tcattacacc ggaaaactga
     8041 tcgacggcac cgtgtttgac agctccgttg ctcgcggcga gccagctgaa ttcccggtta
     8101 acggcgtgat ccctggctgg attgaagcac tgactctgat gccggtaggt tctaaatggg
     8161 aactgactat cccgcaggaa ctggcatatg gcgagcgtgg cgcaggcgcg tccatccctc
     8221 cgttcagcac cctggtgttt gaagtcgaac tgctggaaat cctctaagca gcgcattctg
     8281 ttcccctcga accagagggg agcagacatt cagcaataaa cccttcagtt tgccaaacgg
     8341 cgctattttg tgttgcaaag accccgtaag cgtgtatttt tgtgagctgt ttcgcgttat
     8401 caccgtgata tgacactcac tttaaacata aaattaacat tagatctaaa tcttagtatt
     8461 cataccgcgt attgttacct aatatcgatg agtcccgata cagattcgtc gtatcataga
     8521 ctgactaaag gccgtagagc ctgaacaaca cagacaggta caggaagaaa aaaacatggt
     8581 agatcaggta aaagtcgttg ccgatgatca ggctccggct gaacagtcgc tacggcgcaa
     8641 tctcacaaac cgacatattc agcttattgc cattggcggt gccattggta cagggttgtt
     8701 tatggggtcc ggcaaaacga ttagccttgc cgggccgtcg atcattttcg tttatatgat
     8761 catcggtttt atgctctttt tcgtgatgcg ggcaatgggg gaattgctgc tttcgaatct
     8821 ggaatacaaa tcttttagtg acttcgcttc cgatttactc gggccgtggg caggatattt
     8881 caccggctgg acctactggt tctgctgggt tgtaaccggt atggcagacg tggtggcgat
     8941 cacggcttat gctcagttct ggttccccga tctctccgac tgggtcgcct cgctggcggt
     9001 gatagtgctg ctgctgacgc ttaacctcgc caccgtgaaa atgttcggtg agatggagtt
     9061 ctggtttgcg atgatcaaaa tcgtcgccat tgtgtcgctg atagtcgtcg gcctggtcat
     9121 ggtggcgatg cactttcagt caccgaccgg tgtggaagcg tcattcgcgc atttgtggaa
     9181 tgatggcggc tggttcccga aaggtttaag tggcttcttt gccggattcc agatagcggt
     9241 tttcgctttc gtggggattg agctggtagg tacaacagct gcggaaacca aagatccaga
     9301 gaaatcactg ccacgcgcga ttaactccat tccgatccgt atcattatgt tctacgtctt
     9361 cgcgctgatt gtgattatgt ccgtgacgcc gtggagttcg gtagtcccgg agaaaagccc
     9421 gttcgttgaa ctgtttgtgt tggtaggttt gcctgcggct gccagcgtga tcaactttgt
     9481 ggtgctgacc tctgcggcgt cttccgctaa cagcggtgtc ttctctacca gccgtatgct
     9541 gtttggtctg gcccaggaag gtgtggcacc gaaagcgttc gctaaactct ctaagcgcgc
     9601 agtacccgcg aaagggctga ccttctcttg tatctgtctg ctcggcggcg tggtgatgtt
     9661 gtatgtgaat cccagcgtga ttggcgcgtt cacgatgatt acaaccgttt ccgcgattct
     9721 gtttatgttt gtctggacga ttatcctttg ctcgtacctg gtgtatcgca aacagcgtcc
     9781 tcatctgcat gagaagtcga tctacaagat gccactcggc aagctgatgt gctgggtatg
     9841 tatggcgttc tttgtgtttg ttctggtgtt gttgacactg gaagatgaca cccgccaggc
     9901 gctgctggtt accccgctgt ggtttatcgc gctggggctg ggctggctgt ttattggtaa
     9961 gaagcgggct gctgaactgc ggaaataatc gcattatcat gctggatggc gcaatgccat
    10021 ccagctttta gatcactcac ccgccagcgc gcgcgggaat agtacattgt tttccagact
    10081 gatgtgctcc atcaggtcat caatcagttc attaatgccg ttatacatcg ctttccaggt
    10141 ggtgcaggct tccggcggcg gtgtgacgtt attggtggtg tgtttgatca cttccagcag
    10201 ttcgcccgct tcatcgtgct cgctttccat tacgctgatt ggccccattg cctggctgcc
    10261 catgccttgt ttaatcatcg ggaagagaat ttgctcttct ttcatcatgt ggctggaaag
    10321 ctcttcatgc agcatagtca ggtattttgt cagccctttt ggcacacttg gtttgtcggc
    10381 gtgaacgcgc tcgactttag tcgcttgcag gatcagctcc ggcagttgct cgcggtggcg
    10441 atcgtggtag cgcacgatga tatggtcgat gatttctgcc agcggggcgc tacgccagtc
    10501 tttctcaatc ggttgttcag caagctttgc cagttcggct tcaatgacgt caacatccag
    10561 ttctttacgt gccgccgcgc gcgccagcgt ctgcttaccg ccacagcagt aatccatatc
    10621 atatttacga aacagagccg aagcgcgagg aatagagagc gccagttcac ctaaaggttg
    10681 gtcgcgataa gccatagctg atacctcatt cttaataaga taagatgtat tttaaatgca
    10741 tctttatggc aaatgctata acccttacgt agcagaggct tatttaactc actgccagcg
    10801 tgtcaacttt ctgagacaga ttcagttaaa ggtttttctg gttttgctct gactgcaatg
    10861 accacgccaa ccactaacag cgcgataccg cttagcgtca ttagcggcgg catttgctgg
    10921 cgtaacaaaa aggtgtacag caagcctgcc agcgtttcga aaacaatcag cggcccgaga
    10981 atcactgtcg gtaatcgctg gctggcgacg ttccagcaga gtgcgccaac ccatgagcaa
    11041 agcacggcta tcgcaaccat cagactaata aacaccagcg gacgggggcc aaagggtagg
    11101 gagaagcccg gcgtttgcgt attcagccag taacaggcga cgagatagcc gatgagagaa
    11161 accggcagcg tgaccagcgc ctgcgccgtc gcccacatca tcggatgttt gtcgggattc
    11221 tcccgcagcc agcgggcgtt gcgcagggca taccatgccc agcagaccac ggaaactaac
    11281 gccagcacga tgccagaggt ataacgtgcc cagtcaaaat cggggagtcc gtggtttaac
    11341 tcagcaatat tcacacacgc caggccgatg ccaatacaaa ccagtgccgg ggcgagtttt
    11401 ccccacgcga gtttgccgtc gcgctggcta taaagcagat tggcaaaaac gggaatcacc
    11461 accggcaggg tgccaataat catcgtggaa acaggcgcgc cagtacgttg aatggcactg
    11521 gcaaggcaga agtaatagat gaggttgccc atcatggtga gcatcaaggc ggtaagccag
    11581 tcgcgacgcg ccaactgacg cagacgcacg cgtcccagcc aggcaatggg cagcgcaatt
    11641 aaccctaacg ccagataacg ccccatcgac tgcaacatcg ccgggtattc cggcacgatc
    11701 aacggcccga caaaaataag cccccacatc aaccctgcta acagggcgta cagcacgccg
    11761 ctaatcatta ctggcatcca ttggtctgtc agaagaaagt tcaggaagca gacatgattg
    11821 ctcccggcat cccgtcatta tcagagagga tgcgggagga tgccacttta attaactagt
    11881 tattaacatt aaaaagatgg cttacgcttt cggctaacgt tgtcgttggg cggccaatca
    11941 atttgctaag cgttttgctg tcatcaaaca gaccgccttt cgatgcgcca acgtcagaat
    12001 ccgccagcat atccgccagt ccatcaggca gtccgacgct tttcagtgcc gcagcgaaat
    12061 cagcttcgct caggttttga taggtaacct gtttgccgct ctgtttggtg agttctgccg
    12121 ctaactgtgt cagcgtccag gcactatcgc ccgccagttc gtaaaccttg ccttcgtgac
    12181 cggcttcgct aatcacgcgt gccgcagcta ccgcataatc agcccgcgtt gctgaggcaa
    12241 ttttgccatc gcctgcggca ccgataaata cgccgtgctc cagtgctgcc ggggcgctgg
    12301 cgaggtagtt ttcggtgtac cagccgttgc gcagcagggt gtaaacgatg ccagaatcag
    12361 ccagcatttt ctccgtctcg atgtgctcat cggcgaggcc gagcggggag gaatcggcat
    12421 gtagcaggct ggtataagcg ataaatttca cgccggccgc ctttgcggca ttaataacat
    12481 tacgatgctg cggggcacgt tgacccactt cgctggaaga gatcagcagt agtttttcca
    12541 ctccctgaag tgcagatgtc agtgcggctt catcgccgta atcagcctga cgcacggtaa
    12601 tgccttgtgc tgtcagtgcc tgtgcttttg ccggattacg aacgatagcc actatttggc
    12661 tggcaggaac cgttttcatc aaggattcaa taacatagtg accaagttgg ccagtggcac
    12721 cagtaatagc gatcatggga agtctccatc gttttgtctt gtgtttaaat acgctaacac
    12781 ttaaacttac ttttagtaag tacgtacaaa aaggtaagta tgagatgagt caggttagcc
    12841 tgtcgcaaca actgaaagag ggaaatctct tcgctgaaca gtgtccgtcg cgcgaggttt
    12901 tgaaacacgt caccagccgt tggggggtgt tgattctggt ggcgctacgc gaaggtactc
    12961 atcgctttag cgacctgcgg cgcaaaatgg gcggggtgag tgaaaagatg cttgcgcagt
    13021 cgttacaggc attagaacag gatggttttc ttaaccgtat cgcgtatccg gtggtgccgc
    13081 cgcatgtgga atatagcctc acgccgctgg gcgagcaggt gagcgaaaag gttgctgcac
    13141 tggcggactg gattgagttg aatttgcccg aggtgttagc ggtgcgggat gaacgtgcgg
    13201 cataactgcc ggatgcgctg cgcttatccg gactacggtt catggcttgt agggcggata
    13261 agatgcgtca tcatcgcatc cgccaataaa tgccggatgc ggttgatgat ttgtaggccg
    13321 aaaaagaagt gttttactta ctcagatcca cctgataaat cgcaaacccg atatcatcgg
    13381 tcgcgacttt attcatcgga tactgccctt tctctttaat aaacactgcg gctttatctg
    13441 acggagaggt ttcgaaacgg atatccagtt tcttatcgcc agctattggt gctaaacgcc
    13501 agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca gcaatccacg
    13561 ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg tcgcccgtac
    13621 cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac atggcgttcg
    13681 gatcaatcgg cttgccgtta aaggtcaggt tcttaatcct ttccgcattg gcattaatca
    13741 tctggcactc gccgtcataa cgggcgggct gggtaacatc aatctgataa ttcacaccat
    13801 caataacatc aaagttatag gtgcggaaac catcccagtt gatgagtgac tgtggtttcg
    13861 tgctgttagg atcaatctgg ttaaactgtc cggcggagca ctccagccac tctttcacct
    13921 ctttaccgct ggctttcacc acaatcagcg tattggggta gagataaaga tcggcggcat
    13981 tacggaaggt caactggcct ttttccacct ccacatagct tgccgggtca tttttgcgac
    14041 caccaacttt aaacggtgcg gcagctgaaa gcactggcag ttttgccaga tccggatcgc
    14101 cctgaatgta atgctcgaca tacgcttttt gcgcgttgtt caccacctgc acggtcggat
    14161 cgtcctgcac aagcgccaga tagctataca tattgtcggc agatttaccg attggcttgc
    14221 tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc ttgctgtctt
    14281 ccgccgcgag ggatttttta ttcgcgatgt cgtaaatcgg ccgtgcttcc gctttcgcct
    14341 gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccaca ccaagatgat
    14401 cgccccacat gcctggcatt accgccggaa caccattcag cgtgcctttc gcgatatcag
    14461 ccccttcgat atcagcaaaa tctttacctg ggaaaacggc gtgagcatgg ccaaacataa
    14521 tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc atcactttat
    14581 acggatcggc agacagcccg gaatgcgcca gaacgacaac gacatcggca cctttctcgc
    14641 gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc actttcccgg
    14701 ataaattagc tttatcccag cccatgattt gtggcgggac gacgccaata taaccaatct
    14761 tcagcgtctg tttttttccg tctttatcga ccacttcggt gtctttaatt aaatacggtg
    14821 taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa gggaatttcg
    14881 ctcccgccag cgcatttttc agataatcca gaccgtagtt aaactcgtgg ttgccaagcg
    14941 tgccgacggt atagtccagc gtatttaatg ccttatagac cgggtgaata tcacctgctt
    15001 ttaaaccttt cgccgacatg taatctgcaa gcggactccc ctgaatcaaa tcgccgttat
    15061 cgaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt gcggtacgta
    15121 ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc atgttgctat
    15181 gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca ctggcggcaa
    15241 tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt ttatcatcgg
    15301 gaatacgaaa gaaaagggag aataaacgtc ttacttatag aacagtgaag aatgccacaa
    15361 ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac gatagggtat
    15421 aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt tagatcaagt
    15481 atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg ggacgaaacc
    15541 gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata ttgccgcaca
    15601 caccgttatc atggacggtt tacgtacgct gacaccggat attccggtgc tttctgaaga
    15661 agatcctccc ggttgggaag tccgccagca ctggcagcgt tactggctgg tagacccact
    15721 ggatggtact aaagagttta ttaaacgtaa tggcgaattc accgttaaca ttgcgctcat
    15781 cgaccacggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg taatgtacag
    15841 cgcggcagaa ggcaaagcgt ggaaagaaga gtgcggcgtg cgcaagcaga ttcaggtccg
    15901 tgatgcgcgc ccgccactgg tcgttattag ccgatcccat gcagatgcag agctgaaaga
    15961 gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc tgaaattctg
    16021 cctggtggcg gaagggcagg cgcagctgta cccgcgcttc ggaccaacga atatttggga
    16081 caccgccgct ggtcatgccg tggctgtagc tgccggagcg cacgttcacg actggcaggg
    16141 taaaccgctg gattacactc cgcgcgagtc gttcctgaat ccggggttca gagtatctat
    16201 ttattaatta atttatgcag caggtccatc acttgctgca cctcttcctg agtaagcgcc
    16261 ccgtctttgg cccattgcac gcgaccgtct ttatccagca ccactacagc ggaactttct
    16321 tcatccagct gccaggcacc gcgcgcgacg ccattgctat cgacaataaa ctgcgaccag
    16381 ggataaagct ttttattact ctccagactg ctgcgcacaa acatgccgga acccggaatt
    16441 gcgtcgtcgg tgttaacaat ggtggtggtc tggtaacgat cgtgcggtaa ctttgctgat
    16501 ttaatcgctt caatcagcgt cgcgtttttc tcttttgcag aggtgcgacc agcaatatgt
    16561 tgcagtactc gcacttttcc cactagctgc gcgctgttcc aggttttgta gctaaactga
    16621 tctttatcaa gcaccaactc gccacgatcg gtaatgccaa tcggcggcac tcgctgaccg
    16681 gtttcgaact gatgtgcgga agccatcatc ggcaacagca ggcaggtgag tgccagaatc
    16741 ttgcgtaggg tcatggtgtt tccttcttat gatatgcagg tgatccgacc acttgggtct
    16801 gagttttaat catatgtgct atttatcgaa tttcccgcaa gtgtgatgcc agtttgcggt
    16861 caagcgcaca aatcatatga aaaatgaatg cttatactga agacggcgct tcggtaaaaa
    16921 gataattctg aataattgta acctttaggt aaaaaaagtt atacgcggtg gaaacattgc
    16981 ccggatagtc tatagtcact aagcattaaa atttgcgcct cataatagtt gggccgattg
    17041 tggcaccgca caggcgtaat actcagcagg agataacaat gaaaattttc caacggtaca
    17101 acccacttca ggtggcgaag tacgtaaaga tcctgttccg tggacggtta tacatcaagg
    17161 acgttggcgc ttttgaattt gataagggta agattcttat cccaaaagtg aaggataagc
    17221 tgcatttgtc agtgatgtcc gaagttaacc gtcaggttat gcgtctgcaa acagagatgg
    17281 cttaaccaaa gtgctatgca gtaataagac ggctcctgat tcaggagccg ttgatgtttc
    17341 tgggggttac gcgacgcttt cttctttatc tttagcgtca ggcaattttg gcgaaagggc
    17401 ggtggccttg ctgtcgattc gggtcaccag cagctggtcg atgcggtagt tatcgatatc
    17461 caccacttca aatttgtagc cggcgaattt caccgaatcg gtgcgtttcg ggatcttacg
    17521 cagcataaac atcataaagc cgccgatggt ttcgtagttg cccgactgcg ggaactcgtc
    17581 aatatccagc acgcgcatga cgtcgtcaat aggcgtgccg ccgtctatca gccatgagtt
    17641 ctcatcacgg gcgacaattt gctcttccag cccctgaccg accagatcgc ccatcagcgt
    17701 ggtcatcacg tcgttgaggg tgatgatccc cactaccagc gcgtactcgt tcatgatcac
    17761 cgcgaagtct tcacctgcgg ttttaaaact ttccaacgcc tctgaaaggg ttaacgtatc
    17821 cggcacaatc agcgtattgc gaatttgtac gccgctgttc agtgccaggc tttggttagc
    17881 cagcacgcgg ttcagcaggt ctttagaatc gacatagccg atgatgtggt caatatcttc
    17941 attacagacg agaaacttag agtgcggatg ttccgccacc ttattcttca gactttgctc
    18001 atcttcgtgg agatcaaacc aaatcacgtt ttcacgcggt gtcattgaag acggaacggt
    18061 acgggattcc agctcaaaga cgttttcaat cagctcgtgt tcctgtttac gtaacacgcc
    18121 cgccagcgca ccggcttcca ctaccgcgta gatgtcatca gaagtgatat catctttacg
    18181 taccattggc agtttgaaaa tacggaagat tatgttcgcc agtccgttga agaaccacac
    18241 cagcggggtg caaacgtaca ggcagaagcg catcgggttg atgatacgca aagccacagc
    18301 ttctggcgca atcataccga tgcgtttcgg ggttaaatcc gcaaacagga taaacatgcc
    18361 agtcactaac gagaaagaga gaataaagct cagttgctca gagagttctg ccgacatata
    18421 gcgggagaac aggctgtgaa aagctggaga aaatgccgca tcaccgacga taccgccgag
    18481 aatcgccact gcgttcagac cgatctggac cacggtaaag aacatgccgg gattttcctg
    18541 catattcaga acgcgttggg catttatatt gccttcatca gccagcagtt taagtttgat
    18601 tttgcgtgag gcggcaagcg agatctcgga catcgagaag aacgcactta cagcgatcaa
    18661 acagagtatg actaaaatac tgtttaacat atcttatccg acctttcagg ccagatcctc
    18721 ggaaggggaa gttgattaat ttgtgtgaaa tacacattga aagccgattc gaagaaagtg
    18781 aaccggcatt ttcagtggct agtatagcgt aaggtactgt aaagccgcca gagggttaaa
    18841 attcagacag ctgaaaaatg aaaaactgcc tgatgcgcta tgccgaacag gcctacgcag
    18901 tccccgcaat atattgaatt tggacgattt tgtaggcctg gcaaggcgtt tacgccgtat
    18961 ccggcataaa caaaatgcac tttatcaaca atctacattt gtacccgtaa cgctatgctt
    19021 ccggcggcag acagacgcca attccgccaa ttccacagta accatacggg tttttatgca
    19081 gatattgctg gtggtcatct tcggcataat aaaacggtgt ggcgttagcg atttccgtgg
    19141 tgatgcgacg atcgtcatcg gcggcaagca tcgccgcctg aaaacgttcc agactggcgc
    19201 gagcggcggc atcctgttct ggggtcagcg gataaatcgc tgaacgatac tgcgtgccgt
    19261 ggtcattgcc ctgacgcatt ccctgggcgg gatcgtgatt ctcccaaaat acttgtagca
    19321 actgctcata gctgatgacg gatggatcgt aaacaatgcg caccgcttcg gcatggccag
    19381 tatcgccgga gcacacttcc cgataagtcg gatttggcgt atagccgccg gtatagcctg
    19441 cggcggtgct gtaaacgccg ggtaactgcc agaacagacg ctccacaccc cagaaacaac
    19501 ccatcgcaaa aatggcaatc tccattccgt caggtacatt ggtcattgag tgaccgttga
    19561 ccgcatgcag tgtggctacg ggcatcgggg tgttacgtcc aggcagggca tcggcggggg
    19621 aaaccagatg ctttttatca aataaactca tggtgtcgct ctcccgaaaa tcggtcattg
    19681 gggttaaggt tgtaacaaga ggcgtatttg cacacaataa ccaccgtgaa taggtctaaa
    19741 gtaaaacata agaaatattt tgggtttagt ctgcttttta atccatatta ctggattttt
    19801 gttaagccgt ttaacggcgt tccaggggca ggaaaaaagg atattcagga gaaaatgtgc
    19861 gctatatccg acagttatgc tgtgtaagct tactctgctt aagcggatct gccgtcgccg
    19921 cgaacgtccg tctacaggtc gaggggttat cgggacagct ggaaaagaac gttcgcgcgc
    19981 agctttctac gattgaaagt gatgaagtga cgccagaccg tcgctttcgc gcacgcgtcg
    20041 atgatgccat ccgcgaaggt ctgaaagcgc tgggctatta ccagccgacc attgaatttg
    20101 atctccgtcc accgccaaag aaagggcggc aggtattgat cgccaaagtc acgccaggcg
    20161 tgccggtgtt aattggcggc accgatgtgg tattgcgcgg cggcgcgcgg accgataaag
    20221 actatttgaa attgctcgat actcgcccgg ctattggtac ggtactgaac cagggcgatt
    20281 atgaaaattt caaaaagtcc ttaaccagca ttgcgttgcg taaaggttat ttcgatagcg
    20341 aatttaccaa agcgcagctg ggcattgcgc tcggcctgca taaagccttc tgggatattg
    20401 attataacag tggcgaacgt taccgctttg ggcatgtgac ctttgaagga tcacaaatcc
    20461 gcgatgaata cctgcaaaat ctggtgccgt ttaaagaggg cgatgagtac gaatcgaaag
    20521 atctggcaga actgaaccgt cgactttctg ctaccggctg gtttaactcg gtggtggtgg
    20581 ctccacaatt tgataaagcg cgcgaaacga aagtattacc attgacgggc gtggtttcgc
    20641 cgcgaacaga aaacaccatc gaaaccggcg tcggttactc tacggacgtg ggaccgcgcg
    20701 tgaaagcgac gtggaaaaag ccgtggatga actcttacgg tcacagtttg accaccagta
    20761 ccagtatttc cgcgccggaa cagatcctcg acttcagcta taaaatgccg ctgctgaaga
    20821 atccgctgga acaatattat ttggtgcagg gcggttttaa gcgcactgac ctgaacgata
    20881 ccgagtctga ctccactacg ctggtggctt ctcgctactg ggatctctcc agcggctggc
    20941 agcgtgccat taacctgcgc tggagtttcg accactttac ccagggtgaa attaccaaca
    21001 ccacgatgct gttttatcct ggggtgatga ttagccgcac gcgttctcgt ggtggcctga
    21061 tgccaacctg gggcgactcg caacgctact ctatcgacta ctccaacacg gcctggggct
    21121 cggatgttga tttctccgtt ttccaggcgc agaacgtctg gatccgcaca ctgtacgatc
    21181 gccatcgttt tgtgacacgc ggcacgctgg gctggattga aacgggtgat ttcgacaaag
    21241 taccgccgga tctgcgtttc ttcgccgggg gcgatcgcag tattcgcggc tacaaataca
    21301 aatctatcgc cccgaaatac gccaacggtg acctgaaagg ggcctcgaag ctgataaccg
    21361 gatcgctgga ataccagtac aacgtgaccg gaaaatggtg gggcgcggtg tttgtcgata
    21421 gcggcgaagc ggtaagcgat attcgccgca gcgactttaa aaccggtacc ggggtcggcg
    21481 tgcgctggga atcgccggtc gggccaatca aactcgattt tgccgtaccg gtcgcggata
    21541 aagacgaaca cgggttacag ttttacatcg gtctggggcc agaattatga gtttatggaa
    21601 aaaaatcagc ctcggcgtgg ttatcgttat cttactgttg ctgggatcgg tggcgtttct
    21661 ggtgggcacc accagcggcc tgcatctggt atttaaagcg gcggatcgct gggtgccagg
    21721 actggatatt ggcaaggtca ccggcggctg gcgtgatctc accttgtctg acgttcgtta
    21781 tgagcagcca ggcgtggcgg taaaagcggg caatctgcat ctggcggtcg ggcttgagtg
    21841 cctgtggaac agtagcgttt gtattaatga tctggcgctg aaagacattc aggtcaacat
    21901 cgacagtaaa aaaatgcctc cttctgaaca ggttgaagaa gaggaagata gcggtccgtt
    21961 ggatctctcc atgccgtatc ccattaccct gacacgggtg gcgctggaca acgtcaacat
    22021 caagattgat gacaccacgg tatcggtgat ggacttcacc tccggcctga actggcagga
    22081 gaaaaccctg accctgaaac cgacatcgct gaaaggcctg ttgattgctt tgccgaaagt
    22141 ggcggaagcg gcgcaggaag aagtggtcga accgaaaatt gaaaatccac agccggagga
    22201 aaagccgctc ggcgaaacgc tgaaagatct cttttctcgc ccggtattgc cggaaatgac
    22261 cgacgtgcat ctgccgctca acctgaacat tgaagagttt aagggcgagc aactgcgcgt
    22321 gacgggcgac acggacatca ccgtgcgcac catgctgctg aaagtgagca gtatcgacgg
    22381 caataccaaa ctggacgcgc tggatatcga ctccaaccag gggatcgtca acgccagcgg
    22441 cacggcgcag ctgtcagaca actggccggt ggatatcacc ctcaacagca cactgaacgt
    22501 ggagccgttg aaaggtgaaa aggtgaagct gaaagtgggc ggtgcgctgc gcgaacagct
    22561 ggagataggc gttaaccttt ccggtccggt ggatatggat ttacgcgccc agacgcgact
    22621 ggcggaagcc ggattgccgc tcaacgtgga agtgaacagc aaacagcttt actggccgtt
    22681 cactggtgag aagcagtatc aggcggatga tctgaaactg aaacttaccg gcaaaatgac
    22741 cgattatgcg atctctatgc gcacggctgt taagggccag gagatcccgc cagccaccat
    22801 tacccttgat gccaaaggta atgaacagca ggtcaatctc gacaaactta ccgtcgcggc
    22861 gctggaaggg aaaacggaac ttaaggcgtt gctcgactgg cagcaggcca ttagttggcg
    22921 cggtgagcta acgcttaacg gtattaacac cgccaaagag atcccggagt ggccgtcgaa
    22981 actcaatggc ttgattaaaa cccgcggtag cctgtacggc ggcacctggc agatggaggt
    23041 gccagaactg aagctgaccg gtaacgtcaa acagaacaaa gtgaacgttg acggcacgct
    23101 gaaaggcaac agttatatgc agtggatgat cccagggctg catctggaac tggggccaaa
    23161 cagtgccgaa gtgaaaggcg agctgggggt aaaagatctc aatcttgatg ccaccatcaa
    23221 cgcgccgggg ctggataacg cgctgccggg gcttggcggt acagcgaaag ggttggtgaa
    23281 agtacgcggc acggtggaag cgccacaact actggcagat atcaccgcgc gcggcctgcg
    23341 ctggcaggaa ctttccgtgg cgcaggttcg cgtggaaggc gatatcaaat ccaccgacca
    23401 gattgcgggt aaactcgacg tacgcgttga gcaaatttcg cagccggatg taaatatcaa
    23461 cctcgtcacc ctgaatgcca aaggcagcga aaagcagcac gagctacagt tgcggattca
    23521 gggcgagcct gtctccgggc agcttaatct ggcaggaagt tttgatcgca aagaagaacg
    23581 ctggaaggga actcttagca atacccgctt ccagacgccg gtcggcccgt ggtcgctgac
    23641 ccgcgatatt gcgctggatt accgcaataa ggagcaaaaa atcagcatcg ggccacactg
    23701 ctggcttaac ccgaatgcgg aactgtgcgt gccgcaaact atcgatgcgg gtgccgaagg
    23761 gcgtgcggtg gtgaatctca accgcttcga cctggccatg ctgaaaccat ttatgccaga
    23821 aaccactcag gccagcggta tcttcacggg taaagcggac gttgcctggg acaccacgaa
    23881 agaggggctg ccgcagggca gtatcaccct ttcggggcgt aatgtgcagg tgacgcaaac
    23941 cgtcaacgat gcggcgctgc ccgtggcgtt tcagacgctg aatctgacgg cggaattgcg
    24001 taacaaccgt gccgaattgg gctggaccat ccgcctgacc aataacggcc agtttgatgg
    24061 acaggtgcag gtgaccgatc cgcaaggccg ccgtaatctt ggtggcaacg tcaatatccg
    24121 taacttcaac cttgcgatga taaaccccat ctttacccgt ggggaaaaag cagcggggat
    24181 ggtgagtgcc aacttgcgtc tgggtggtga tgtgcaaagc ccgcagttgt ttggtcagct
    24241 tcaggttacg ggtgtggata tcgacggcaa ctttatgccg tttgatatgc aaccgagcca
    24301 gcttgcggtc aactttaacg gtatgcgctc gacgcttgcc ggtacagtac ggacccagca
    24361 gggtgaaatc tacctgaacg gtgatgccga ctggagccag attgaaaact ggcgggcgcg
    24421 ggtaacggcg aaaggcagta aagtgcggat caccgtgccg ccgatggtac gaatggatgt
    24481 atcgccagat gttgtattcg aggctacacc aaacctgttt accctcgatg gtcgcgtgga
    24541 tgtcccgtgg gcgcgcatcg tggtgcatga gctgccggaa agcgcggtgg gcgtctccag
    24601 cgatgtggta atgcttaacg ataacctgca accggaagag gcaaaaacgg cgtcgattcc
    24661 gattaacagc aacctgattg tccacgtcgg caacaatgtg cgcattgacg cctttggcct
    24721 gaaagcgcgg ctgacgggcg atctcaacgt cgttcaggac aaacaagggc tgggcctgaa
    24781 cgggcagatc aacatccctg aagggcgctt ccatgcctat ggtcaggatc tgattgtgcg
    24841 taagggtgag ttactgttct ctggtccgcc ggatcaaccg tatcttaata tcgaagctat
    24901 tcgtaacccg gatgctacag aagacgacgt aatcgccgga gttcgcgtca ctggtctggc
    24961 ggacgaaccg aaagcggaga tcttctctga cccggccatg tcgcaacagg ctgccttgtc
    25021 ttatttgcta cgtggacaag ggctggagag cgatcagagc gacagtgcgg cgatgacctc
    25081 gatgctgatt ggtctggggg ttgcacaaag tggtcagatt gtgggtaaaa tcggcgagac
    25141 gtttggcgta agcaatttag cgctcgacac ccagggagta ggcgactcct cccaggtggt
    25201 ggtcagcggc tatgtattgc caggtctgca agtgaaatac ggcgtgggta tatttgactc
    25261 tatagcaaca ctcacgttac gttatcgcct gatgcctaag ctatatctgg aagccgtgtc
    25321 tggtgtagac caggcactgg atttgctcta tcagttcgag ttttagcaat gcgaatattt
    25381 gtctacggca gtttacgcca caaacaaggc aacagtcact ggatgaccaa tgcccagtta
    25441 ctgggcgatt tcagtatcga taactaccag ttgtatagcc tgggccacta tccaggcgca
    25501 gttccgggga acggaacagt acacggtgaa gtttatcgta ttgataacgc cacgctggcc
    25561 gaacttgatg ccttgcgcac caggggcggt gaatacgcgc gccagttgat tcagacgccg
    25621 tacgggagtg catggatgta cgtttatcaa cgacccgtcg atggattaaa gctaattgaa
    25681 agcggcgact ggttagacag ggataagtaa taataaaagc acgccacctt cgggtggcgc
    25741 gaaaattgat gtaatgcaat gaaaaataaa ggattatttt tctcgttgtc cacattttgc
    25801 catagcgata catcttagcc cgtaatgatt cgcgggctaa gcatatacaa aatgttaagc
    25861 ctgatacggg ggtcctatgt ttgataggga aatgataaac gaaaataaag aggcgttttt
    25921 tctgtatgtt aacggtagag gggagttgtc ggctcaatgc gtgataaagg tggctgaaaa
    25981 aggggattat ttacaaggat tctctttaac tcgtcgcgca tttcgtacct ttttgaaaga
    26041 ccgtgttatt cagatgtttt ctagcgacca ggagttgaat aaggttgcgt ctgaaactga
    26101 tttcagtcag tatattgccc cccccgaaaa tcttcaccca aaaagaattc aatttcaatt
    26161 tgttttaccg gatttgacca gtctgataaa tctcgcttaa gtgctcttgc aaaagaaaat
    26221 ggtattgatg ttaagtctga tgttggaaaa agtttgtttt tcctttgtgt aggaaagaac
    26281 aaagggtggc ggaaagttca gaaagccagc gattatggcg taattattct gacagaagaa
    26341 caatttatca attttattga tactggtgaa attcctcttg agcaagactg cgtggttgag
    26401 aatgatatca gtaaatcgct tgagaaggat gttaacactt acaagagtat gatagatgag
    26461 ttctcgcttt ctatccgtac tgtaagggag tttcgccgta gtaatgcgtt gattgcggta
    26521 tttgttgatg gttacgcagc tggttggcgc tttgctgtaa aagagtgtta caaggattct
    26581 ttggatataa aacttacccc agtaaaaaca gagtttttta attatgaaat ttggacgcaa
    26641 ggggattcct ttcagtttaa tcgcggtgat gttttttata gtgatcgcct ggcgtatgat
    26701 tcttattgcg attttttaaa gcttgatgat gctgttgttc ttcaggtgaa atatgagtgc
    26761 ttttctggtt acgattctgt aagtactttt gatggtgtga tggatggtga gttttctttt
    26821 tctcgtatgg aacccagacc tgtccataaa ttacctgtgc tgattgaaac tcaactgtat
    26881 gaccccggaa agattattgt tgatgttttt gttcctgatg ctgcgaaaaa acaattaaaa
    26941 cttaaagaca ccttagagct gaagcaggat gaatttgtct gtttacttca gtcagggtgt
    27001 tgttgggtta aacgaaggga agcaggagat aagaaaaaac ctgaatttat taacctattc
    27061 aaaaaataaa aatatagccc gcatatcgcg ggcaaatgtc tatgtaattc aacggagaaa
    27121 ggg
//
LOCUS       DAZKGF010000056        27120 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZKGF010000056 DAZKGF010000000
VERSION     DAZKGF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.056"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(70..660)
                     /locus_tag="ACHOQV_002206"
     CDS             complement(70..660)
                     /locus_tag="ACHOQV_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase"
                     /protein_id="HFP9630577.1"
                     /translation="MPEKRKHLTRHEVGQILLAADRGIHFERDYCLIQMCFLHGLRVS
                     ELCGMRLSDIDLIGRSVYVRRLKNSLSTQHPLFDNELPALMRWLAVRNRWRDADSDWL
                     FLSQKGGALSRHQVRLLLKRYGELAGVSISAYPHMLRHGCGYALADLGRDTRLIQDYL
                     GHRNIRHTVIYTATNTQRFMNVWGNVRENHTISARM"
     gene            873..1796
                     /locus_tag="ACHOQV_002207"
     CDS             873..1796
                     /locus_tag="ACHOQV_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFP9630578.1"
                     /translation="MRAQYILLLLCAAFPLRAITTPEIAASALTPDCVKYRVVGLCYW
                     LYCTPFGCTVRTSVKVSHFRPDLVVSAYSNTGQNPWAEMSLLSPPLPGVAEGGGDTNP
                     RSASQHSKVRFKNADAIGFPAGDELAKFFTQFGYICSPSSQPFQPYFLSQPDTLAWRS
                     GVPEMTYPEALTPGMREVGQNGDMWGNIYPRAGAINQTHDYKAAAVIAQRAADLVTRT
                     GQPHLYTPLTASSRAGYWPPSPVIEGDSDNHRWQMLTPKKSAACSVFPDGSATDTYAN
                     KLAEDGAYAWTLWRPYKCCPRRGQTFLGSTG"
     gene            1808..3169
                     /locus_tag="ACHOQV_002208"
     CDS             1808..3169
                     /locus_tag="ACHOQV_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFP9630579.1"
                     /translation="MRALFLVLLFFAGVCVAADDYSFRTRGAVDDLLYYQIGGGEAVM
                     PPPVRRSPSPLSLGLGWNADMMCGNFDIDTTVRNQLNGITDGFQQLMGNVIQSATGAV
                     ASLPAMIIQRANPQLYDLLTNGVLQGRLDFDKSLLNCQKMAEKMTDLAADSAWFSGAK
                     AENYQSLAASDNDAIRTDQKAAKEAAEKGKRWVGGEKRGGKGQLPIKVVHDATAAGWN
                     ILNQQPATSTTSLSSSECDGELCSTWTSPEEAAGWMTRVVGEQTISVAQVTDDPDARS
                     GAQAGIGLHPLIDEEKAKILPLLTDLVNGSQKPGFDNLAKASGGSMQLTRGVVEALRD
                     DPDVAVLTERLAGELALARVTEQALLARRTLLAGMREPNIANVKEAQESLGKTTAQLD
                     EELNQLKLELDFRQALTRNTASQILQRKQQRDQLQGQVVEVPDDSDSRLHNLNNPQPD
                     SPR"
     gene            3166..4668
                     /locus_tag="ACHOQV_002209"
     CDS             3166..4668
                     /locus_tag="ACHOQV_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFP9630580.1"
                     /translation="MTANSYFEYVLTLLGWLVNNGVWQTLTATGLFAVPLLAKLVALW
                     LKARGQGADEGNVARLLVVWVEHSLYSALLVIFFAGVPFLNVDINTLTYDSQRTKQCG
                     WSVVQPGDSGYAPLINELSGQSASVPVWWYFTHALGKGITSAAVAVLPCKPDLRQLRF
                     EVQHTRLADPALAQELQDFVNDCYGPSRARLKRLSSDISEEQSREVDWVGSSFYLATL
                     GYYDRDHSRSPRAAWPYDASRDDGLSDTGGGGYPACKEWWSDGDTGLKNRLLKQVDTT
                     LWQQLQKLGQSKEEYEEAVLRSLVSPRNMQVSQGGLVYNGFGGSAGDNLSQTQLLSRV
                     GGIIGAGLSSYALFPAFDNVRQALPMVQAFLEMALVICIPVILLFSAWDLKTVITLSF
                     VQFALFFLTFWWELARWLDNWLMQMMYDSDTHSYFNLWGLQNTSDDLIVNIIMGVMFL
                     VLPAFWLGALTWAGIRIGAAVAGGMGSAVGDIRSAGEQVGKMIVSKTRIP"
     gene            complement(4813..5013)
                     /gene="glgS"
                     /locus_tag="ACHOQV_002210"
     CDS             complement(4813..5013)
                     /gene="glgS"
                     /locus_tag="ACHOQV_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006174923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFP9630581.1"
                     /translation="MNRQDINALKNFDFLARSFARMHALGQPVDIDAVTGNMSDEQQA
                     WFRERYDHYRKQAERARVIELR"
     gene            5347..5637
                     /locus_tag="ACHOQV_002211"
     CDS             5347..5637
                     /locus_tag="ACHOQV_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630582.1"
                     /translation="MSLFDKKPSLMPVVIAPDVWQSAVEFPADPQQVEERLGNLLLVV
                     LLTLRTAGTSRKKVTFTIYCQPPQGDMKVPVALPLSLNYHGHYVQVSMAEQA"
     gene            5703..6608
                     /locus_tag="ACHOQV_002212"
     CDS             5703..6608
                     /locus_tag="ACHOQV_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFP9630583.1"
                     /translation="MDRSNDIYQKVTDQIIAELENGNVPWLRPWRDGEPPFPINALSG
                     RPYHGINVPLLWNSADRQSFASDRWLTFHQVSALGGQVRRGEKSSLAVLYLPRTQEET
                     DALGQPLLDKDGEPKVRHFGIIREFWLFNLSQCDGLPQALSEPFTRPATPVEMAEAIT
                     MNSGVTLRHRRQSKAYYKPATDLVMMPHPQQFESSEAYYATLLHELTHATGHASRLNR
                     PGIAGAERTEALYAAEELVEEMGSAFLCAHCGIQGRLQHASYIGYWLDILRQDKRAVI
                     RASGMARHASEWLLNAVPQPCQLSA"
     gene            6674..7045
                     /locus_tag="ACHOQV_002213"
     CDS             6674..7045
                     /locus_tag="ACHOQV_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630584.1"
                     /translation="MGLDLFFFERKKNAPDESQYARVGYFRKVNPLLKWVDKHVGLVR
                     NQVLLEVTKANLQALLSDLQSLTPDNCGAVYPTIDGFFYGYTDYGEHYWKDVRTVTLW
                     VKETLETFDFERDRLMFYAWW"
     gene            7219..8808
                     /gene="mobH"
                     /locus_tag="ACHOQV_002214"
     CDS             7219..8808
                     /gene="mobH"
                     /locus_tag="ACHOQV_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HFP9630585.1"
                     /translation="MLKWLKRPSQSQPPTPDATLPEGWFAPQSAEQLLATPLRQSCLH
                     QLRQLTPLPDTLFDSWILAAIHRFAALVQLLPASQSHHHSTPGGLLDHSLEVACNAIR
                     LRQGYLLPPEAGAEEQARQASAWTVAVVCVALLHDTGKIAVDMEIHQRDGTRWHSWQG
                     YLAQPYRWQYQSHGRDYHLHPAATAMLINQLIPSPLLDWLAGYPAAFASLIYQLTGHY
                     ERSGALAELVQQADKASVARYLGGDIKTALAQKPSSFPQQLLTALRSLVQNEYRLNNP
                     ECGSDGWLTEDALWLISKTTADRVRAWLLQHGVSSVPENNNRLFDELLTHQLIVANED
                     KAIWRCIIRSDKGWSPVEPLTLLRLTPSVIWGQDDQRPACFDGDVTPVIEAEPAPELS
                     APTQPLPEIADDPRLGDNNNFLQWLKNHTSPSATVNTRNGKVHVVENHLFLISPGIFK
                     TYAAETTGDSGDGWKLAQRMFQESGLALRCNDDSFIWICEVRVPQKKSSQLKGFLMED
                     PKQVLGEKVPVNNPWLKLLRE"
     gene            complement(8864..9517)
                     /locus_tag="ACHOQV_002215"
     CDS             complement(8864..9517)
                     /locus_tag="ACHOQV_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9630586.1"
                     /translation="MKKKLPDQLSRECIIAAMRAYDEGVRHQFSESRLYDVVFEGRSY
                     PPKAIVGIAASNMTGVEFSPEDFSGGIKSKCVKLLTDQGFQIVEKHLGNEQPAISIFP
                     DELPPQSVFTEGAATQVTVNRYERDRKARQAALAWHGCLCKVCGIDMMKVYGEIAKGF
                     IHIHHLIPLSDIKENYSLDPKKDFIPVCPNCHAMLHRRNPPFSPEELKSIIEYHAAL"
     gene            complement(9629..9895)
                     /locus_tag="ACHOQV_002216"
     CDS             complement(9629..9895)
                     /locus_tag="ACHOQV_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005289972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630587.1"
                     /translation="MSPRTHRQLVSVEVMWPAQTLPLPLQQVVEALNQGETPDQIIIR
                     MNQRGLLAWREDASAQDTHDIFQVRLDNQNEARFLCRYVTLPLH"
     gene            complement(10022..10363)
                     /locus_tag="ACHOQV_002217"
     CDS             complement(10022..10363)
                     /locus_tag="ACHOQV_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012776626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9630588.1"
                     /translation="MNVTSPKMLSQAIRDARKQRNLTQRETAASMGMKQSTVSEFENH
                     PEGTSLDTLFKLLAALDLKLQVVSWRGKKTLLMAGSRSGDLWKYEQKGHGAIRTEPPF
                     SLAYHLDFQST"
     gene            complement(10774..11262)
                     /locus_tag="ACHOQV_002218"
     CDS             complement(10774..11262)
                     /locus_tag="ACHOQV_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9630589.1"
                     /translation="MAKKTAPLVPGTNQLLAEFGERLKLARLRRKLTAKRVAERAGMS
                     PMTLRSLESGGTGVTIGAYLSVMQILGLEKDLNKLAAEDEQGRQLQDSRLINKTRVSR
                     PAAISHTNRHTGNVIKNDAFDKQFSTSQEKSIDSEVKTEVVTSASLAALLKPIKKPRK
                     GS"
     gene            complement(11570..12379)
                     /locus_tag="ACHOQV_002219"
     CDS             complement(11570..12379)
                     /locus_tag="ACHOQV_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFP9630590.1"
                     /translation="MTNIFTLMNPISSATETLLSQINRICTAQGIPFFIAGATAREIL
                     LHHIHSRSIGRRTRDIDIAVFVDSWPRFDALKNAFLAEGAEVVQGNAHRMIVKGIELD
                     IIPFGGVAEGNQIAWPPEREIIMAVDGFAEVFSHTVVVRLDNSENIPFCSLPGLALLK
                     LFAWRDRGHGSTKDATDLFKIICEYGVIENERIYESPVEGERLNWDPIRMGAFLLGSD
                     IATISEHSSLQDLLHLDKERLIDVIVRQHDVGDAGIEQIINDFWDGMASDG"
     gene            complement(12333..13331)
                     /locus_tag="ACHOQV_002220"
     CDS             complement(12333..13331)
                     /locus_tag="ACHOQV_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HFP9630591.1"
                     /translation="MKEEQLLSDAIENLPKGFHLQYEHSGRHGKHDAWGKLTGPDGES
                     VIFALEVKFIHRKESLMAVREQMVRFPADFPVLLVCNRLTSALAEYCADSQINFIDTA
                     GNARIQVPGLYVRIEGRYEKKPVSASSRFAEGVMKLLFVLLSSPETLNETYRSLAGKA
                     GISLGMVSKAFDYLEAQRYFRKSKKGRRLINEGELEALWLKDYATALRPKLDSLSLSA
                     PVSWDELTLASGEYWSGEIAAAKLSGGYLIPENGVIFTSHPLLQRRKELGLKPVPGGK
                     LQLIAGFWGNFVLNHKAEAMLCVAELLASGDDRNWETARIINDKYLHLNESDLFSY"
     gene            complement(13959..15206)
                     /locus_tag="ACHOQV_002221"
                     /pseudo
     CDS             complement(13959..15206)
                     /locus_tag="ACHOQV_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(15429..15513)
                     /locus_tag="ACHOQV_002222"
     tRNA            complement(15429..15513)
                     /locus_tag="ACHOQV_002222"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15477..15479),aa:Leu,seq:caa)
     gene            15709..16728
                     /gene="ahr"
                     /locus_tag="ACHOQV_002223"
     CDS             15709..16728
                     /gene="ahr"
                     /locus_tag="ACHOQV_002223"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFP9630592.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTILNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            16858..18360
                     /gene="yjgR"
                     /locus_tag="ACHOQV_002224"
     CDS             16858..18360
                     /gene="yjgR"
                     /locus_tag="ACHOQV_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFP9630593.1"
                     /translation="MSEPLLIARTSDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAEEGTSSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLPWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(18479..19561)
                     /gene="lptG"
                     /locus_tag="ACHOQV_002225"
     CDS             complement(18479..19561)
                     /gene="lptG"
                     /locus_tag="ACHOQV_002225"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP9630594.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(19561..20661)
                     /gene="lptF"
                     /locus_tag="ACHOQV_002226"
     CDS             complement(19561..20661)
                     /gene="lptF"
                     /locus_tag="ACHOQV_002226"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP9630595.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(20724..20774)
                     /gene="ytgA"
                     /locus_tag="ACHOQV_002227"
     CDS             complement(20724..20774)
                     /gene="ytgA"
                     /locus_tag="ACHOQV_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFP9630596.1"
                     /translation="MSIIILATISTTQENK"
     gene            20928..22439
                     /gene="pepA"
                     /locus_tag="ACHOQV_002228"
     CDS             20928..22439
                     /gene="pepA"
                     /locus_tag="ACHOQV_002228"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFP9630597.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYRGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            22573..23016
                     /gene="holC"
                     /locus_tag="ACHOQV_002229"
     CDS             22573..23016
                     /gene="holC"
                     /locus_tag="ACHOQV_002229"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFP9630598.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            23016..25871
                     /gene="valS"
                     /locus_tag="ACHOQV_002230"
     CDS             23016..25871
                     /gene="valS"
                     /locus_tag="ACHOQV_002230"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP9630599.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESTQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLSADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(25925..>27120)
                     /gene="yjgN"
                     /locus_tag="ACHOQV_002231"
     CDS             complement(25925..>27120)
                     /gene="yjgN"
                     /locus_tag="ACHOQV_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFP9630600.1"
                     /translation="AQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLPW
                     ALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCVL
                     TLLLLVFLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTVF
                     FISTKMLPANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKV
                     KLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQR
                     KMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALV
                     VISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVMP
                     SLPFL"
BASE COUNT         6630 a         7253 c         7102 g         6135 t
ORIGIN      
        1 aagatagtcc gttaccaaaa ggggtctcag aaaaattttg acagtttgct aaatttttct
       61 gatactactt tacattctgg cactaattgt gtggttttct ctcacgtttc cccagacatt
      121 cataaacctc tgcgtatttg tcgccgtata aatcaccgta tgccggatgt tccggtgccc
      181 cagataatcc tgtatcagac gggtgtcgcg ccccagatct gccagcgcgt aaccgcaacc
      241 gtgtctcagc atatgcggat aagcggaaat gctgacacct gccagttctc cgtaacgttt
      301 gagtaacaat ctaacctgat ggcgcgacag cgcaccaccc ttctgcgaca ggaacaacca
      361 gtctgaatcc gcatcccgcc agcggttgcg taccgccagc cagcgcatca gcgcaggcag
      421 ttcattatca aacagcggat gctgcgtgga gagactgttt ttcaaccgcc ggacatacac
      481 cgagcgcccg atcagatcga tatcagacag gcgcatcccg cacagttcac tgacccgcag
      541 gccgtgtaaa aaacacatct gaatgaggca ataatcgcgc tcaaaatgga tccctctgtc
      601 tgctgcaagc aatatctgtc ccacttcatg acgagtgaga tgctttcgtt tttcaggcat
      661 cagccttgct ccttatcaaa ataaaaaatg aaaaaaaatc aggccagaac tgccttcaca
      721 gtccaggcct gaaccagaga ataccaccag aaacaacgtc tggtgatgat gtaaaactca
      781 acaattttct gtaacggctt ccagtttcat accgttgatg aacagatatt gcggtacacc
      841 cgacagaaga aatatctgct gtcggaggcg cgatgcgagc ccaatacatc cttctgctgc
      901 tgtgcgctgc tttccccctc agggcgatta ctacgccgga gatcgcagcc tctgcgctta
      961 cgccggactg tgtgaaatac cgggtggtcg gcctgtgcta ctggctgtac tgcacgccgt
     1021 tcggctgtac ggtacgcacg tcggtgaagg tcagccattt ccgcccggat ctggtggtgt
     1081 cggcttacag caacaccggg cagaatccgt gggcggagat gtcgttactc agcccaccgt
     1141 tgcccggtgt tgccgaaggc ggcggcgata cgaatcctcg ttctgccagc cagcacagca
     1201 aggtccgctt taagaatgcc gatgccatcg gcttcccggc gggtgatgaa ctggcaaaat
     1261 ttttcaccca gtttggctat atctgctcac cttcatcgca gccttttcaa ccgtactttc
     1321 tgagtcaacc cgatacgctg gcgtggcgca gcggtgtgcc ggaaatgaca tacccggagg
     1381 cgctgacgcc gggcatgcgg gaagtcggtc agaacggcga tatgtggggc aatatctacc
     1441 cgcgagccgg agccatcaac cagacgcacg actataaagc cgccgccgtt atcgcccagc
     1501 gcgcggccga tctggtgacc cgtaccgggc aaccgcatct ctacacgccg ctgaccgcct
     1561 catcgcgcgc cgggtactgg ccgccctccc cggttatcga aggcgacagc gataaccatc
     1621 gctggcaaat gctcacgccg aaaaaatccg ccgcctgttc ggtgttcccg gatggcagtg
     1681 ccactgacac ctacgccaac aaactggcgg aggatggcgc gtacgcctgg acgctgtggc
     1741 ggccctataa atgctgtccg cgccgggggc aaacctttct cggcagtacg ggttaacgga
     1801 ggcggtgatg agagcccttt tcctggtctt attgtttttc gccggggttt gcgtggcggc
     1861 agacgactac agtttccgca cgcgaggcgc ggttgacgac ctgctttact accagattgg
     1921 cggcggtgaa gcggtcatgc cgcctccggt acgccgtagc ccctcgccgc ttagcttagg
     1981 tcttggctgg aacgccgata tgatgtgcgg caatttcgac atcgacacca ccgtgcgcaa
     2041 ccagctcaac ggcatcaccg acggcttcca gcaactgatg ggcaacgtta tccagagtgc
     2101 caccggggcg gtcgccagcc tgcccgcgat gattatccag cgcgccaatc cgcagcttta
     2161 tgatctgctg accaacggcg tgctgcaggg gcggctggat tttgataagt ctctgctgaa
     2221 ctgccagaaa atggcggaga agatgaccga tttggcggcc gattccgcct ggttttccgg
     2281 agccaaagcg gagaactacc agtccctcgc cgccagcgat aacgatgcca tccgcaccga
     2341 ccagaaagcg gcaaaagagg ccgccgaaaa gggtaaacgc tgggtgggcg gtgaaaagcg
     2401 cggcggcaaa ggccagctgc cgattaaggt agtccatgac gccaccgccg caggatggaa
     2461 tatcctcaac cagcagcctg ccaccagcac tacttctctt agctcctctg aatgcgacgg
     2521 ggaactttgt agcacctgga catcccctga agaggccgcc gggtggatga cccgcgtcgt
     2581 tggcgagcag accatcagcg tggcgcaggt caccgacgat ccggatgcgc gttccggcgc
     2641 gcaggcgggg atcggcctgc atccgctgat cgacgaagaa aaggcgaaga tcctgcccct
     2701 cctgacagat ctggttaatg gcagtcagaa acccggcttc gacaatttgg cgaaagcttc
     2761 cggcggttct atgcaactga cccgcggcgt ggtcgaggcg ctgcgcgacg atccggacgt
     2821 tgccgtcctc accgaacggc tggcgggaga actggcgctg gcccgcgtca ccgagcaggc
     2881 gttgctggcc cggcgtacgc tgctcgcagg tatgcgtgaa cccaatatcg ccaacgtcaa
     2941 agaggcgcag gagagcctgg gcaaaaccac cgcccaactg gatgaagagc tgaaccagtt
     3001 gaagctggaa ctcgatttcc gccaggcact gacgcgcaat accgccagcc agatcctgca
     3061 acgcaaacag cagcgcgacc agcttcaggg acaggtggtg gaggtgccgg acgacagcga
     3121 cagccgtctg cacaacctca ataacccgca gccggacagc ccgcgatgac cgccaacagc
     3181 tatttcgagt acgtgctcac cctgctcggc tggctggtga ataacggcgt gtggcagacg
     3241 ctgaccgcca ccgggctgtt cgccgtgccc ctgctggcaa aactggttgc cctgtggctg
     3301 aaagcgcgcg gccagggggc cgatgaaggc aacgtcgccc gcctgctggt cgtctgggtg
     3361 gagcactcgc tctacagcgc actgctggtg atcttcttcg cgggtgtgcc gttcctcaat
     3421 gtcgatatca acaccctcac ctacgacagc cagcgcacaa agcagtgcgg ctggtcggtg
     3481 gtacagcccg gcgattccgg ctacgcacct ttgattaatg aactcagcgg ccagagcgct
     3541 tccgttccag tgtggtggta cttcacgcat gcactgggca aaggcatcac cagcgccgcc
     3601 gtcgccgtat tgccctgcaa gccggacctg cgccagttgc gctttgaggt gcagcacacc
     3661 cggctcgccg atccggcact ggcgcaggag ctacaggatt tcgttaacga ctgctacggg
     3721 ccgtcgcggg caaggctcaa gcggctgagt agcgatatta gcgaggagca aagccgggaa
     3781 gtggactggg tgggatcgtc tttttacctc gccacgctag gctattacga ccgcgatcac
     3841 tcccgttcgc cccgcgccgc ctggccttat gacgccagcc gcgacgacgg tctgagcgat
     3901 acgggtggcg gtggctatcc tgcgtgtaag gagtggtggt ccgatggcga tacggggctg
     3961 aaaaaccgcc tgctgaagca ggtcgatacc acgctctggc agcaactgca aaaactggga
     4021 cagtctaaag aggagtatga agaagcggta ctgcgctcgc tggtcagccc gcgcaatatg
     4081 caggtatcgc agggcggact ggtgtacaac gggtttggtg ggagtgcggg ggataacctc
     4141 agccagacgc agttgctgag tcgtgtgggc gggatcatcg gcgcggggct ttcgagctac
     4201 gccctgtttc cggcgtttga taacgtccgc caggcgctgc cgatggtgca ggcgttcctc
     4261 gaaatggcgc tggtgatttg tattccagta attctgctgt tctcggcatg ggatcttaaa
     4321 acggtcatca cgctgagctt tgtgcagttc gccctgttct ttctcacctt ctggtgggaa
     4381 ctggcccgct ggctggataa ctggctgatg cagatgatgt acgacagcga tacccactca
     4441 tacttcaacc tctggggcct gcaaaacacc tccgacgatc tgattgtaaa tatcattatg
     4501 ggcgtgatgt tcctggtgtt acccgcattc tggctggggg cgctgacgtg ggcgggcatc
     4561 aggatcggcg cagcagtcgc tggggggatg ggcagcgcgg tcggcgatat ccgcagcgct
     4621 ggcgaacagg tcgggaagat gatcgtcagt aaaacacgca ttccctgaag atttcgtact
     4681 gacggtgtga tgaacagggc ttgtagctga gtttatgcgc accgcataaa ctgtcgtgtc
     4741 agtgacaatc tcgtggtagg tagctggagt ctgaaaaaac gcaacgggcc gttaagcccg
     4801 ttgttcgtta catcaccgca gttctatcac tctcgcccgc tcagcctgct ttcggtagtg
     4861 gtcgtatcgc tcccggaacc atgcttgctg ttcatcactc atattgccgg taacggcatc
     4921 gatatcaact ggctggccca gggcgtgcat acgggcaaaa ctgcgcgcca gaaaatcaaa
     4981 attctttaat gcgtttatat cttgtcggtt cattagcata tccctccagt cattgtgttt
     5041 actgttcaag gtcatatgct tttctgattt cagtatccgc ctgaaaaaga tcgttttttg
     5101 agcgatgcgt gcgcatcaat ctacctgtgt tcacatttca ggaacgacaa aactcttcag
     5161 ccccagtaat gattttcccc tcccttttat cacccgcgca gaaaacacta ccgccagcca
     5221 tgcagtaagt tcgccatgta tccggaaggt gcgaacggcg cagatgcgca ggatacccgt
     5281 accattccat cccgcaggga aacgccctgc gaagggtgtt ccctgctcat ttacaggaga
     5341 aacaccatgt cgcttttcga taaaaaaccg tcgttaatgc cggtggttat cgcccctgat
     5401 gtctggcaat ccgcagtgga atttcccgcc gacccgcagc aggtggaaga acggctgggc
     5461 aatctgttgc tcgtcgtact gctgaccctg cgcaccgccg ggacatcccg caagaaagtc
     5521 acctttacga tctactgcca gccgccgcag ggcgatatga aagtccctgt cgcattaccg
     5581 ctatcgctca actatcacgg ccactatgtg caggtctcga tggctgaaca ggcataacct
     5641 ctccccgccg gagtcgcttc cggatggagg aggctccggc ttctttccat gaggaccaca
     5701 cgatggacag aagcaacgat atctaccaga aagtcaccga ccagattatc gctgagctgg
     5761 aaaacggcaa tgtgccgtgg ctccgcccgt ggcgcgatgg cgagccgccg ttcccgatca
     5821 atgcgttatc cggtcgtccg tatcacggca tcaatgtacc gctgctgtgg aacagcgccg
     5881 acaggcagag cttcgccagc gaccgctggc tgacgttcca tcaggtcagc gcgctcggcg
     5941 ggcaggtgcg ccggggggag aaatcctctc tggcggtgtt gtatctgccg cgcacgcagg
     6001 aagagaccga cgcactcggc cagccgttgc tggataaaga cggcgagccg aaggtgcggc
     6061 atttcgggat tattcgtgaa ttttggctgt ttaacctgtc gcagtgcgac ggcctgccgc
     6121 aggcgctgtc cgagccgttt acccggcccg caacgcctgt ggagatggct gaagccatta
     6181 cgatgaatag cggtgtgacg ctgcgccatc gccgccagtc gaaagcgtat tacaaacccg
     6241 cgaccgatct ggtgatgatg ccgcacccgc aacagtttga aagcagcgag gcgtactacg
     6301 ccacgctgct gcatgaactg acgcacgcta ccggacacgc ttcacgcctt aaccgccccg
     6361 gtattgccgg agcggaacgc accgaagcac tctacgctgc tgaagagtta gtggaggaga
     6421 tgggtagcgc cttcctgtgc gcccattgcg gcattcaggg caggctccag cacgccagct
     6481 atatcggcta ctggctggat atcctgcgcc aggacaagcg ggcggtgatc cgcgccagcg
     6541 gtatggcgcg ccacgccagt gaatggctgc tcaacgctgt accacaaccc tgtcagctta
     6601 gcgcctgaca gcccacccgc acggggagcc ttccccgccg ggaagttgct cccctttttc
     6661 tgaggatcaa tgtatgggac tcgatctctt ctttttcgaa cggaaaaaaa acgcgcccga
     6721 tgaaagccag tacgccagag tgggttattt ccgcaaggtg aatccgctgc ttaaatgggt
     6781 ggataaacac gttgggctgg tgcgcaacca agtgctgtta gaagtcacca aagcaaattt
     6841 gcaggcgctt ctgagcgacc tgcagagcct gacgccggat aactgcggcg cggtttatcc
     6901 gacgattgac ggctttttct atggttatac cgattatgga gaacactact ggaaagacgt
     6961 tcgcaccgtc actctctggg tgaaggagac gctggagacg tttgattttg agcgagacag
     7021 gctgatgttt tatgcctggt ggtagtatta aagtggccga caaaacgtcg gtcactttgc
     7081 ctcgtcggcc agaaatacag cgccagatat acacatgaga gcataccgtt tttactaata
     7141 aaaatgtaaa aggcccaaag ggagacactg caaaggaagg gcgacaaggg caaaggggcc
     7201 tgcccggaaa ggggaaatat gctgaagtgg ctaaaaagac catcccagag ccagccacct
     7261 acgccagacg ctacgctgcc ggagggctgg tttgcgccac aatccgcaga acaattgctg
     7321 gcgacgccgt tacgccagtc ctgtttgcac cagttacggc aactgacccc gctgccggat
     7381 accctgtttg atagctggat cctcgcggcg attcatcgct tcgccgcgtt ggtacaactg
     7441 cttcccgcct cacaaagtca tcaccattcc actcccggcg ggttgctgga tcattcgctg
     7501 gaggttgcct gcaacgccat ccgactgcgc cagggttatc tgctaccacc ggaagcgggc
     7561 gcggaagaac aggcccggca ggccagcgca tggacggtcg cagtggtctg cgtggcatta
     7621 ctgcacgata cgggaaaaat cgccgtggat atggagattc atcaacgtga tggcacccgc
     7681 tggcattcgt ggcagggtta tctggcacag ccttatcgct ggcagtacca gagccacggt
     7741 cgcgactatc atctgcatcc ggcggctacc gcaatgctga ttaatcagtt gataccctcg
     7801 ccattactgg actggctggc cggatacccg gcggcttttg cgtccctgat ttaccagctc
     7861 accggacact acgaacggtc tggcgcactg gctgaactgg tgcagcaggc cgataaagcc
     7921 tctgtcgccc gatacctcgg cggcgatatc aaaaccgcgc tggcgcaaaa gccctcttcc
     7981 ttcccccagc aacttcttac agccttgcgg tcgctggtac agaacgagta ccggctcaat
     8041 aatcccgaat gcggatccga tggctggctg acggaggatg cgctgtggct tatcagcaaa
     8101 accaccgccg accgggttcg cgcctggctg ctccagcatg gagtttccag cgtgccggaa
     8161 aacaataacc gcctgtttga cgaactgctg acgcatcagc ttattgttgc caacgaagat
     8221 aaagcgattt ggcgctgcat tatccgatcg gataaaggct ggtcgcccgt tgaaccgctg
     8281 acgctactgc gcctgacgcc gtctgtaatc tgggggcagg atgatcaacg tccggcctgt
     8341 ttcgatggtg atgtaacgcc ggtaattgaa gccgaacccg ctcctgaact tagcgcaccg
     8401 actcaaccgt taccggagat agcagacgat ccgaggcttg gtgacaataa caatttcctg
     8461 cagtggctga agaaccacac tagtccttca gccacggtga atacccggaa cggcaaggta
     8521 catgtggtgg aaaaccatct tttcttgatt tcgccgggga tctttaaaac atacgcagca
     8581 gaaaccacag gcgatagcgg cgacggctgg aaactggcgc aacgaatgtt tcaggagtca
     8641 gggctggccc tgcgctgtaa tgatgacagt tttatctgga tctgcgaggt tcgggttcca
     8701 caaaagaaaa gtagtcagtt gaaagggttt ctgatggaag atccgaagca ggtgttgggg
     8761 gagaaagtgc cggtgaataa tccgtggctg aagttgctgc gcgaatagct atttatatca
     8821 aaatactctc ttccaccgag ctacagcgaa tgggctttaa tacctaaaga gccgcatgat
     8881 attcaataat tgatttcaat tcttcaggcg aaaaaggcgg attacgccga tgcaacatgg
     8941 cgtggcaatt tgggcagacg ggaataaaat cttttttggg atccagagag taattctctt
     9001 tgatgtctga tagcggaatc aggtggtgta tatggataaa acctttcgct atctcgccat
     9061 acaccttcat catatcaata ccacatactt tgcacagaca accatgccag gctaatgcgg
     9121 cctgtcgggc cttacggtcg cgttcatagc gattaaccgt tacctgcgta gctgcacctt
     9181 cagtaaatac gctctgcggt ggcagttcat caggaaaaat actgatggcg ggctgctcgt
     9241 tacccaaatg tttctcaacg atctggaacc cttgatcggt aagcaacttg acgcatttcg
     9301 acttgatacc accactaaaa tcttcagggg aaaactcaac gccagtcata tttgaagcag
     9361 caataccgac tatagctttc ggtggataac tgcgcccttc aaagaccaca tcatacagcc
     9421 gggactcgct gaactggtgg cgtacacctt catcataggc acgcatagcc gcaataatgc
     9481 attccctgga aagctggtct ggcagtttct tcttcattta caactcatca tccctgaaac
     9541 gacatttgct aactctttat tttatagcat attaatttat ccggtgataa acagcactct
     9601 tcaatttcgc gttaccggga taggggcatc agtgcagcgg caacgtaacg tagcggcaga
     9661 gaaatcgcgc ctcattctgg ttgtccagac gtacctgaaa gatatcgtgt gtatcctgcg
     9721 cggatgcatc ctcacgccac gccagcagac cccgttggtt catgcggata ataatctgat
     9781 cgggcgtttc gccctgattc aatgcttcga ctacctgttg taggggaagc ggtagcgtct
     9841 gagcaggcca catcacttca acacttacga gctggcggtg tgtccgaggt gacatcggtg
     9901 gtttcctcca tggctggaat ttagcggtta attagccatg aaataggggc attttttact
     9961 gtttcaacta ctgccgagat ggctacggaa tacgcgctga ctgatgcaaa aatagcttct
    10021 cttatgtgct ctggaaatcc agatggtacg ctagtgaaaa tggtggttcc gtccggatag
    10081 cgccatggcc tttttgttca tatttccaca gatcaccact cctgctccca gccatcagaa
    10141 gagttttttt acccctccag cttacgacct gaagcttaag gtccagagcg gcaagcagct
    10201 taaagagcgt atccaggctg gtcccctcag gatggttctc aaactctgaa accgttgact
    10261 gtttcatgcc catacttgct gcagtttcac gctgggtcag gttacgttgc ttgcgtgcat
    10321 ccctgattgc ctgagacaac attttgggtg aggttacgtt cattttatcc cccaggatag
    10381 atattttgag catatcccct ataggggata aaaattcata ttctcagcgg agaaaagatt
    10441 aataactaaa tgaattaaaa ttacttttca tggaaattaa ggggggacga tattcctcga
    10501 gccagtaaat atgaacattg ccagatggca gaacgcgcca ttttacaagc attttccata
    10561 acagatataa gactgattga gcggttggat taagcatgac gatatgttta aaaaaaccag
    10621 ttttatcgac ataacgccaa aatatgttta cagcatcgac atatcaacgc catatgtaca
    10681 ttttttggca ttttttaaac atatcatcag caggctttat tttttgctat cgaaatctac
    10741 agtccctcgt ttcaatacaa aaattccagc gtgtcagcta ccttttctgg gtttctttat
    10801 tggttttagc agtgcagcaa gagatgcaga tgtcacgact tctgttttaa cttcagaatc
    10861 gattgatttt tcttgtgatg tcgagaattg cttgtcgaag gcatcgtttt ttatcacgtt
    10921 tccggtatgc ctgtttgtgt gagagatagc agcaggacga gaaacacgcg ttttatttat
    10981 tagccgggaa tcctgaagtt ggcgtccctg ttcatcctca gcggcaagtt tgttcagatc
    11041 cttttcaaga cccaaaatct gcataactga caaataagcg ccgattgtca cgcccgtccc
    11101 gcctgattca agagaacgca aggtcattgg cgacatcccg gctcgctcag caacccgctt
    11161 cgctgtgagc ttacgtcgca gtcgcgccag cttaagccgt tcgccaaact cagcaagcaa
    11221 ctgattagta ccgggaacta agggagccgt ttttttagcc atagtgataa tattttatct
    11281 tttcaatacc tatatagcca taatattagc gcatcatgcc gaaggtgcgg tgcgccgtgg
    11341 aaggtggaac atcaagttta cctgcgttct ccactatctt tgatgggaac cagcaagcct
    11401 ttgaccttca gtttgatgat gtcgcgactg actctgggct ggcattattg ataacataag
    11461 cgcatcttaa tcgggcagtt ggcgttacgc cctgccattg ttcacaataa taccgttcat
    11521 gattcatgaa caaaactctg ttgtgaacat ttcatctaaa actacatcct caaccatcac
    11581 ttgccatacc atcccagaaa tcattgatga tctgctcaat cccagcatca cccacgtcat
    11641 gttgtcgtac tatcacatcg atcaggcgtt ctttatcaag atggagtaaa tcctgcaagg
    11701 atgagtgttc actaattgtc gctatatcgc ttcccaacag aaaagcgccc atacgtatgg
    11761 gatcccagtt caggcgttcc ccttcgacgg gcgattcata gatgcgttca ttctcgataa
    11821 caccatattc acagataatc ttaaagagat cggtcgcgtc tttagtactc ccatgcccac
    11881 gatcacgcca tgcaaaaagt tttaacagag ccagtcctgg caaggagcag aacggaatat
    11941 tttcgctgtt atccagccgc acaactacgg tatgagaaaa gacttcggca aatccatcca
    12001 cagccattat gatctcacgt tctggtggcc aggcaatttg atttccttca gcaacaccac
    12061 caaaaggaat aatatctagc tcgatccctt tgacaatcat acgatgggca ttaccttgga
    12121 caacttctgc cccttcagca aggaaagcat ttttaagcgc atcaaatctc ggccagctat
    12181 ccacaaagac ggcaatatca atatcacggg ttcggcgacc aatacttctg ctgtgtatat
    12241 gatggagcag aatttcacga gccgttgcac ctgcaataaa aaacgggatc ccctgagcgg
    12301 tgcaaatacg gttgatctga ctcagtaacg tttcagtagc tgaagagatc ggattcatta
    12361 aggtgaagat atttgtcatt gattatcctt gctgtttccc agtttctgtc atccccgctt
    12421 gccagcagtt cagccacgca tagcatcgcc tcggctttgt gattcagtac aaagttcccc
    12481 caaaaaccgg cgatcagctg tagtttcccc cccggaacag gttttagtcc cagttccttt
    12541 cgcctttgta acaacggatg ggatgtaaaa atcacgccat tttctggtat caggtatcct
    12601 ccgctcagct ttgccgccgc aatctcgccg ctccagtatt ccccggacgc aagcgtcaac
    12661 tcatcccatg aaacaggcgc ggataaagaa agcgaatcca gcttagggcg caaagctgta
    12721 gcataatctt tcagccacaa agcctctaac tcgccttcat tgattaagcg acgccccttc
    12781 ttcgatttgc ggaaatagcg ttgcgcttcg agataatcaa aagccttgct gaccattccc
    12841 agagagatac ctgcctttcc ggccagactg cggtaagtct cgttcagggt ctctgggcta
    12901 gacagcaaaa caaaaagaag cttcatcacc ccttcagcaa aacgactgct ggcggaaacg
    12961 ggtttctttt catagcggcc ttcgatccgc acatacaagc ctggaacctg gatacgggca
    13021 ttccctgccg tatcgataaa gttgatctgg ctgtcggcgc aatattccgc cagtgcagac
    13081 gtcaggcggt tacacaccag cagcacagga aaatccgcag gaaagcgaac catttgttcg
    13141 cgaacagcca tcagtgactc tttacggtgg ataaatttta cttccagagc aaatatcact
    13201 gactcaccat ccggccctgt tagcttaccc caggcatcgt gcttaccatg tctaccgcta
    13261 tgttcatact gcaaatggaa tccttttggc agattctcta tcgcatcaga caaaagctgt
    13321 tcttccttca tttgttcaca cctcataact gttcatgaat catgaacaaa acaatgttat
    13381 gaacaaataa agaaagaagc aacgtaaatg atcaaaaaaa gaccgccaga aatgcgatgc
    13441 cttattcaac cttccaaacg tcattttcca ttaaagaaag aagggtaaag agtagttgga
    13501 cgcattcttt cagggttgag taaccattcc tactcccccg actccacctc ctcctgcgcc
    13561 gccatcagaa ccaactctga cttcgaaatc agcaggtctt tcggcctccc acctctcaac
    13621 ctgcgattag ccgagccaaa ccagatagcc agcccccacg gtgttttacg ctccttaaac
    13681 agtgaaagaa tcaaacggac aaccttgaga ggccgcattt tctcatccag aacataatca
    13741 gggtataaat cctcaccatc gaccttcagc gagaatatct tcccggcggc tttccagcct
    13801 ttcggaccag catcaggatc ggcggaacga aagcccgctt ttgcggataa ttcagacgcc
    13861 gtcagccatt ccgagtcagc cagtattctg gctttcagct cctctagctg ccggtcattg
    13921 cgctcctgga aactcatatg gtcgagtgcg cctcccggcg actttttgaa gccatacggc
    13981 acaacatgcc tgaaccggtt tgcatccaga taatccgccc accactgcat catctcccaa
    14041 cgttcttcga gatgctgcgc cttgtggata tacgccgcac gcacgcgctt gcgctcctga
    14101 tggctcatct ggcgctctac cgcgtcgctt gaccacagcc ccgactccac cagcgcgcta
    14161 caggccatag tgcgaaaacc gtggccacag acatctttct gggtgtcgta gcccatcacc
    14221 cgcagcgctt tgttgatggt gttttcactc atcggcttgc ggtaattgtg atcgccgggg
    14281 aacaccagtt cagttcccgg tcggcaatgc tgccgcacct cgtgcagcag ttcgatggcc
    14341 tgacgtgaca atgggaccag gtgtggggag tgcatcttcg ccccacgcgc tgaatattta
    14401 acgccgggga tcggctcacg ctcagcgggg atggtccaca tggcgttacg cagatcaatt
    14461 tcatcccagc gggcaaagcg cagttcgctg gagcggataa acagcagcag tgccagtttt
    14521 accgccagtt gagtcagttg acggcctttg taatcgtcga ttctttccag gaaatcaggg
    14581 ataagattca gatccaacgc cgggtgatgc cgtactttgg gcgtggagac cgcgccggtt
    14641 aaatctgatg cagggttatg atcgataatg ccgttctgga cggcgtagcg catcacgcag
    14701 gcagtgcgct gctgaagccg ggaagcaaca tccagtttgc ccgctttctc cacttccttg
    14761 atgggcacca gcagatcttt gaccttcatt ttggtgatgt cacagctgcc gttcgtgggg
    14821 aatacgtaaa gctcgaaata acgcagcacc cgcttcttat gttccgccga ccagttcacg
    14881 ttactggtga cccattcacg tgccacagac tcaaacgtat gttccggcgt tatcgcaagg
    14941 cgttctgctt ttcggcgtgc actcggatcg atgccggaaa tcagcaataa ttgtgcctct
    15001 tcacgcatgc gtcttgcctg tgcaagcgtc aaaagcggat aagggcctaa agcgagcttt
    15061 ttctctttgt ttacaaagcg gtacttgatg taccagcgtt ttgaaccgtt agggttaacc
    15121 agcaggtaaa gtccatgcga gtcactgagc ttatacggct tgccacaagg cttggcgtgg
    15181 actatagcgc gatctgttag tgccatttgg gggcctcact attatcgaac tacgtctaag
    15241 cccccaattt ggcccccaaa tcgcgtggat gtcaatggat gaaaaatgac caacacggaa
    15301 agaccacaaa ataaatatgg ttattgtgga aaaataaatt acctttgaat tacaaacgga
    15361 tatggacgaa tttggacgga aatggacaca aaaaaagcca cccttaggta gctaattatt
    15421 gcatcacttg gtgccgaagg ccggactcga accggcacgt atttctacgg ttgattttga
    15481 atcaactgcg tctaccgatt tcgccacttc ggcactgaag aggtatgcgg aaaacgttgt
    15541 ggattatacc tgtcgcacgc caccatgcaa gcgccagctt gctcgcacct cgctaagtgc
    15601 tgaaattttc agcattgcat accgcgatgt gtaacctttg tcacactcca ggcgccccgc
    15661 cctgccatgc tctacacttc ccagaccaca ccagagaagg ataaaaacat gtcgatgata
    15721 aaaagttatg ccgcaaaaga ggctggcggc gaactggaag tttatgagta cgatcccggt
    15781 gaactgaagc cacaagatgt tgaagttcag gtggattact gcgggatctg ccattccgat
    15841 ctgtcgatga tcgacaacga atggggattt tcacagtatc cgctggttgc cgggcatgag
    15901 gtgattgggc gcgtggttgc attgggtagc gccgcgcagg ataaaggttt gcaggtcggt
    15961 cagcgtgtcg ggattggctg gacggcgcgt agctgtggtc actgcgacgc ctgtattagc
    16021 ggtaatcaga tcaactgcga gcaaggtgcg gtgccgacga ttttgaatcg cggcggtttt
    16081 gccgagaagt tgcgtgcgga ctggcaatgg gtaatcccac tgccagaaaa tatcgacatt
    16141 gaatctgctg ggccgctgtt gtgcggcggt atcacggtct ttaaaccact gttgatgcac
    16201 catatcactg ccaccagccg cgtcggcgtg attggtattg gcgggctggg gcatatcgct
    16261 ataaaacttc tgcacgcaat gggatgcgag gtgacagcct ttagttctaa tccggcgaaa
    16321 gagcaggaag tactggcgat gggtgccgat aaagtggtga atagccgcga tccgcaggca
    16381 ctcaaagcac tggcggggca gtttgatctc attatcaaca ccgtcaacgt cagcctcgac
    16441 tggcagcctt attttgaggc gctgacgtat ggcggtaatt tccatacggt cggtgcggtt
    16501 ctcacgccgc tgtctgttcc ggcctttacg ttaattgcgg gcgaccgcag cgtctctggt
    16561 tctgctaccg gcacgcctta tgagctgcgt aagctgatgc gctttgccgc ccgcagcaag
    16621 gttgcgccga ctaccgaact gttcccgatg tcgaaaatta acgacgctat ccagcatgta
    16681 cgcgatggta aagcgcgcta ccgcgtggtt ctgaaagccg acttctgatc cctttgccgg
    16741 gtgcctgtcg cccggcgttt aaatctcttt ggaaggcgtc tcgcaatctg ctgaatttag
    16801 cggtgaagag tgcgcagccg ttgcctatac tccaggcagg aaactggagg atatctcatg
    16861 agcgaacccc tgttaattgc ccgcacgtcg gacacagaac tgtttttatt gccgggaatg
    16921 gcgaatcgtc acgggctgat tactggcgca acggggacgg gtaaaaccgt tacgctgcaa
    16981 aaactggcag agtctctatc tgaaatcggc gtgccggtgt ttatggccga tgtgaaaggt
    17041 gatttaacgg gagtagcgga ggaaggtact tcttcggaaa aactgctcgc aaggcttaaa
    17101 aatatcggcg taaatgactg gcaacctcat gctaatccgg tggtggtgtg ggatatcttt
    17161 ggcgagaaag gccacccggt gcgggcgacg gtttcggatc tggggccgct gttgctggcg
    17221 cgactgttga atctcaacga tgtgcaatct ggcgtgctga atattatctt ccgcattgcg
    17281 gacgatcagg gattgttgct gctcgacttt aaagatttgc gggcgattac ccagtacatc
    17341 ggcgataacg ccaaatcctt ccagaatcag tacggaaata tcagtagcgc atcggttggt
    17401 gccatccagc gcggattact gtcgctggaa cagcaaggcg cagcacactt ctttggtgag
    17461 ccgatgctgg atatcaaaga ctggatgcgc accgatgcca acggtaaagg cgttatcaat
    17521 atcctcagcg ccgagaaact ttatcagatg ccgaaactgt acgccgccag cctgccgtgg
    17581 atgctttcag agttatatga acaattgcct gaagcgggcg atctggagaa accaaaactg
    17641 gtgtttttct tcgacgaagc acatctgctg tttaacgatg cgcctcaggt actgctggat
    17701 aagattgagc aggtgataag gcttattcgc tcaaaaggcg tgggcgtctg gttcgtttcg
    17761 caaaacccgt ctgatattcc ggataatgtg ctcgggcagc tcggtaatcg tgttcaacac
    17821 gctttgcggg cttttacgcc caaagatcag aaagcagtaa aagctgcggc gcaaaccatg
    17881 cgggccaatc cggcatttga taccgaaaag gcaatccagg aactggggac cggcgaggcg
    17941 ttaatctcgt ttctcgatgc aaaaggaagt ccttctgtgg tggaacgggc gatggtgatc
    18001 gcaccttgtt cgcgaatggg gccggtgacg gaagatgagc gtaatggcct gattaatcac
    18061 tccccggtgt atggcaaata tgaagatgag gtggaccgcg agtccgccta tgagatgctg
    18121 caaaaaggct ttcaggccag taccgagcag caaaataatc cccccgtgaa agggaaagag
    18181 gtggcggtgg atgacggtat tcttggtgga ttgaaggata ttttgtttgg cactaccgga
    18241 ccacgcggcg ggaagaaaga tggtgtggtg caaacaatgg cgaaaagcgc cgctcgccag
    18301 gtgacgaatc agattgtacg cgggatgttg gggagtttgc tgggggggag aaaaagataa
    18361 gtcagacgtg atctgtgccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    18421 cgaactcgta tttgactttg tcaacaataa taaagggagc tttcgctccc ttgttagatt
    18481 acgattttct cattaacagc cacaggctga ttaagaagaa gctggcgctt ggcaacagtg
    18541 cgccgatgat cggcgggatg ccataaacca acgtcagcgg gccgaagatc tggtccagta
    18601 cgtagaagac aaaaccgaag ctgataccgg tgaccacacg cacgcccatc ggtacgctac
    18661 gcagtgggcc aaagatgaac gacagcgcca tcagcatcat caccgccacg gatagcggct
    18721 ggaagatttt gctccacatg ttgagctgat aacgtccggc atcctgaccg ctcgacttca
    18781 gatacttcac gtagttgtgc aaaccactga tggagagtgc atccgggtcc agcgccacca
    18841 cgcccagttt gtctggcgtg aggttggttt tccaggtgcc gctcaccgtc tgcgaaccgg
    18901 taatctgttt cggattggtc aggtcagatt catcaacctg cgacagacgc cagactttat
    18961 gttccgggtc aaacttcgcg gtagcggcat aacgtacgga ttgcagacga cgattctcgt
    19021 taaaggcata aatactgatg ccacctaact cctcgtcacc tttaacccgc tcaatgtaga
    19081 cgaagttgtt gccatctttc gcccataagc cttgctgggt agagagcaac gagccgccgt
    19141 acatcgcctg cgcacggtag ttacgcgcca tctgctcgcc ctgcggcgcg acccattcgc
    19201 caatcgccat cgtcagcaag accagtggaa tggcggtttt catcaccgac agcgctacct
    19261 gcatacgggt aaaaccagaa gcctgcatca ccaccagttc gctgcgctgc gccagcatcc
    19321 caagaccaag caacgcccca agcagagccg ccatcgggaa gaagatttgc acatctttcg
    19381 gcacgctcag caaggtatac attcctgcgc ctaacgcgtc gtaactcccc tgcccggctt
    19441 ttttcagctg atcgacaaac ttgataatgc ccgacagcga caccagcatg aacagcgtca
    19501 tcatgatggt ggtgaaaata gttttaccga tatagcggtc aagtacgcca aaaggttgca
    19561 tcacaccgct cctttacgcg aaaaactggc gcgcaggcgg cggaccggca cggtgtccca
    19621 aaggttgaga acaatcgcca aagccagata aatcaggtta acggtccaca tccacagcgt
    19681 cgggtccagc ttacctttac cgccgttcga tttcagggag gtctggatca ggaagaaaag
    19741 tagatacagc agcatggctg gcagcatcga cagtacgcgt ccctgacgcg ggttaaccac
    19801 gctcagcggt acgaccataa gtgccatcat aaacacggtg aataccaacg tgatacgcca
    19861 gttcagttct gcacgggcac gatcggtgtc agtgttccac aatgtgcgca tgtccatctg
    19921 gtcggtatcg ttcgggtcga gcgccaccgc ctggtgacca atgatcgcct gataatcctg
    19981 gaagtccgta atgcggaaat cacgtaacag tgcagtgcct tcgaagcgcg ttccctggtt
    20041 gagagtgacg acctgggagc cgtcgcgcag ctgggttaaa tgtccggaat cggccaccac
    20101 cacagaagga cgtgcattac cttttggtcg aatttgcgcg aggaacacat ctttgaaatc
    20161 gctgccgtca acgctttcga tgaacagcac cgagctgcca ttagtcgctt gctggaattg
    20221 cccttgcgcc agcgccgcca tgccagggtt cgctttcgct tctgctaaca cttcatcctg
    20281 atgacgcgat gaccacggtc ccgcccacat cacgttaacc gccgcgacga ttgccgtgaa
    20341 taccgcaagg atcattgccg ctttcaccag aaccgctttg ctcaggccgc aggcatgcat
    20401 taccgtaatt tcactttcgg tatacagttt gcccagcgtc atcagcagcc cgaggaacag
    20461 gcttaatggc aggataagct gcgccatttc cggcacgccc aacccgagaa gggagagcac
    20521 cagattcgcc ggaatatcgc cgtcaaccgc tgcgccgagg atcctcacta acttttgaca
    20581 gaagaagatc aaaagcaaga tgaagagtat cgccagctgg cttttgagcg tctcccgcac
    20641 cagatatctt atgattatca ctttaaatac gcccgtaaaa actcgtcttt tgcaggattt
    20701 tagcttgttt catggcttaa acgtcattta ttctcttgag tcgtcgaaat cgtcgctaag
    20761 ataattatac tcaacggatt cacctctcag attttgttct gacgtgccaa tgccgtaata
    20821 acgttaagat taacacgaag tcatcgcaac agcggacatg agttacgaaa gcttgcaatt
    20881 ctatctgtag ccaccgccgt tgtctttaag attcaggagc gtagtgcatg gagtttagtg
    20941 taaaaagcgg tagcccggag aaacagcgga gtgcctgcat cgtcgtgggc gtcttcgaac
    21001 cacgtcgcct ttctccgatt gcagaacagc tcgataaaat cagcgatggg tacatcagcg
    21061 ccctgctacg tcggggcgaa ctggaaggaa aaccggggca gacactgttg ctgcaccatg
    21121 ttccgaatgt actttccgag cgaattctcc ttattggttg cggcaaagaa cgtgagctgg
    21181 atgagcgtca gtacaagcag gttattcaga aaaccattaa tacgctgaat gatactggct
    21241 cgatggaagc ggtctgcttt ctgactgaac tgcacgttaa aggccgtaac aactactgga
    21301 aagtgcgtca ggctgtcgag acagcaaaag agacgctcta cagtttcgat cagttgaaaa
    21361 cgaataagag cgaaccgcgt cgcccgctgc gtaagatggt gttcaacgtg ccgacccgcc
    21421 gtgaactgac cagcggtgag cgcgcgatcc agcacggtct ggcgattgcc gccgggataa
    21481 aagcagcgaa agatctcggt aatatgccgc cgaatatctg taacgccgct tatctcgcct
    21541 ctcaggcgcg ccagctggct gacagctaca gcaagaatgt catcacccgc gttatcggcg
    21601 aacagcagat gaaagagctg ggaatgcact cttatctggc ggtcggtcag ggttcacaaa
    21661 acgaatcgct gatgtcggtg attgagtaca gaggcaacgc gtcggaagat gcacgcccaa
    21721 tcgtgctggt gggtaaaggt ttaaccttcg actccggcgg tatctccatc aagccttcag
    21781 aaggcatgga tgagatgaag tacgatatgt gcggcgcggc ggcggtttac ggcgtgatgc
    21841 gtatggtcgc ggagctgcaa ctgccgatta acgttattgg cgtgctggca ggctgtgaaa
    21901 acatgcctgg cgggcgtgcc tatcgtccgg gcgatgtgtt aaccaccatg tccggtcaaa
    21961 ccgttgaagt gctgaacacc gatgccgaag gccgcctggt actgtgcgac gtgttaactt
    22021 acgttgaacg ttttgagccg gaagcagtga ttgacgtggc gacgctgacc ggtgcctgcg
    22081 tgatcgcgct gggtcatcat attactggtc tgatggcgaa ccataatccg ctggcccatg
    22141 aactgattgc cgcgtctgaa caatccggtg accgcgcatg gcgcttaccg ctgggtgacg
    22201 agtatcagga acaactggag tccaattttg ccgatatggc gaacattggc ggtcgtcctg
    22261 gtggggcgat taccgcaggt tgcttcctgt cacgctttac ccgtaagtat aactgggctc
    22321 acctggatat tgcaggaacc gcctggcgtt ctggtaaagc aaaaggcgca accggtcgtc
    22381 cggtagcgtt gctggcacag ttcctgttaa accgcgctgg gtttaacggc gaagagtaag
    22441 tttgcgtttt accctcaccc taaccctctc cctaagggag agggaaccga tcgagcgcaa
    22501 tagaaattca ttgctcatct ttcagggtga ggaatgcagc aatatttaaa tccaccacaa
    22561 gaagccccat atatgaaaaa cgcaacgttc taccttctgg acaatgacac caccgtcgat
    22621 ggcttaagcg ccgttgagca actggtgtgt gaaattgccg cagaacgttg gcgcagcggt
    22681 aagcgcgtac tcatcgcctg tgaagatgaa aagcaggctt accggctgga tgaagccctg
    22741 tgggcgcgtc cggcagaaag ctttgttccg cataatttag cgggagaagg accgcgcggc
    22801 ggtgcgccgg tggagatcgc ctggccgcaa aagcgtagca gcagcccgcg cgatatattg
    22861 attagtctgc gaacaagctt tgcagatttt gccaccgctt tcacagaagt ggtagacttc
    22921 gttccttatg aagattctct gaaacaactg gcgcgcgaac gctataaagc ctaccgcgtg
    22981 gctggtttca acctgaatac ggcaacctgg aaataatgga aaagacatat aacccacaag
    23041 atatcgaaca gccgctttac gagcactggg aaaagcaggg ctactttaag cctaatggcg
    23101 atgaaagcca ggaaagtttc tgcatcatga tcccgccgcc gaacgtcacc ggcagtttgc
    23161 atatgggtca cgccttccag caaaccatca tggataccat gatccgctat cagcgcatgc
    23221 agggcaaaaa caccctgtgg caggtcggta ctgaccacgc cgggatcgct acccagatgg
    23281 tcgttgagcg caagattgcc gcagaagaag gtaaaacccg tcacgactac ggccgcgaag
    23341 ctttcatcga caaaatctgg gaatggaaag cggaatctgg cggcaccatt acccgtcaga
    23401 tgcgccgtct cggcaactcc gtagactggg agcgtgaacg cttcactatg gacgaaggcc
    23461 tgtccaatgc ggtgaaagaa gttttcgttc gtctgtataa agaagacctg atttaccgtg
    23521 gcaaacgcct ggtaaactgg gacccgaaac tgcgtaccgc tatctctgac ctggaagtgg
    23581 aaaaccgcga atcgaaaggt tcgatgtggc acatccgcta tccgctggct gacggcgcga
    23641 aaaccgcaga tggtaaagat tatctggtgg ttgcgactac ccgtccggaa accctgctgg
    23701 gcgataccgg cgtggccgtt aacccggaag atccgcgtta caaagatctg attggcaaat
    23761 atgtcattct gccgctggtt aaccgtcgta ttccgatcgt tggcgacgaa cacgccgaca
    23821 tggaaaaagg caccggctgc gtgaagatca ccccggcgca cgactttaac gactatgaag
    23881 tgggtaaacg tcacgccctg ccgatgatca acatcctgac ctttgacggt gatatccgtg
    23941 aaagcaccca ggtgttcgat accaaaggta acgaatctga cgtttattcc agcgaaatcc
    24001 cggcagagtt ccagaaactc gagcgttttg ccgcacgtaa agcggtcgtt gctgcggttg
    24061 acgcactcgg cctgctggaa gaaattaaac cgcacgacct gaccgttcct tacggcgacc
    24121 gtggcggcgt agttatcgaa ccgatgctga ccgaccagtg gtacgtgcgt gccgatgtcc
    24181 tggcgaaacc ggcggttgaa gcggttgaga acggcgacat tcagttcgta ccgaagcagt
    24241 acgaaaacat gtacttctcc tggatgcgcg atattcagga ctggtgtatc tctcgtcagc
    24301 tgtggtgggg tcaccgtatc ccggcatggt acgacgaagc gggtaacgtt tatgttggcc
    24361 gcaacgaaga cgaagtgcgt aaagaaaata acctgagcgc ggacgtcgcg ctgcgtcagg
    24421 acgaagacgt tctcgacacc tggttctcct ccgcgctgtg gaccttctcc acgctcggct
    24481 ggccggaaaa caccgacgca ctgcgccagt tccacccaac cagcgtgatg gtatccggct
    24541 tcgacatcat cttcttctgg attgcccgca tgatcatgat gaccatgcac ttcatcaaag
    24601 atgaaaatgg caaaccgcag gtgccgttcc acaccgttta catgaccggc ctgattcgtg
    24661 atgacgaagg ccagaagatg tccaaatcca agggtaacgt gattgacccg ctggatatgg
    24721 ttgacggtat ttcgctaccg gaactgctgg aaaaacgtac cggcaatatg atgcagccgc
    24781 agctggcgga caaaatccgt aagcgcaccg agaagcagtt cccgaacggc attgagccgc
    24841 acggtactga cgcgctgcgc ttcaccctgg cggcgctggc ttctaccggt cgtgacatca
    24901 actgggatat gaagcgtctg gaaggctacc gtaacttctg taacaagctg tggaacgcca
    24961 gccgctttgt gctgatgaac acagaaggtc aggattgcgg cttcaacggc ggcgaaatga
    25021 cgctatcgct ggcggaccgc tggattctgg cggagttcaa ccagaccatc aaagcgtacc
    25081 gcgaagcgct ggacagcttc cgcttcgata tcgccgcagg cattttgtat gagttcacct
    25141 ggaaccagtt ctgtgactgg tatctggagc tgaccaagcc ggtaatgaac ggtggaacag
    25201 aagccgaact gcgcggtact cgccatacgc tggtgactgt actggaaggt ctgctgcgcc
    25261 tcgcgcatcc gatcattccg ttcatcaccg aaaccatctg gcagcgtgtg aaagtacttt
    25321 gcggtatcac tgccgacacc atcatgctgc agccattccc gcagtatgat gcatctcagg
    25381 ttgatgaagc cgcactggcc gacaccgagt ggctgaaaca ggcgatcgtt gctgtacgta
    25441 acatccgtgc agaaatgaac atcgcgccgg gcaaaccgct ggagctgttg cttcgtggtt
    25501 gcagcgcgga tgcagagcgt cgcgtaaatg aaaaccgtgg cttcctgcaa acgctggcgc
    25561 gtctggaaag catcaccgtg ctgcctgccg atgacaaagg tccggtttcc gttaccaaga
    25621 tcatcgacgg cgcagagctg ctgatcccga tggctggcct catcaacaaa gaagatgagc
    25681 tggcgcgcct ggcgaaagaa gtggcgaaga tcgaaggtga aatcagccgc atcgagaaca
    25741 agctggcaaa tgaaggcttt gtcgcccgcg caccggaagc ggttatcgcg aaagagcgtg
    25801 agaagctgga aggttatgca gaagcgaaag ccaagctgat tgaacagcag gctgttatcg
    25861 ccgcactgta atcaacacag tgaagaaaac gaaggccgga gcatgctccg gcctttttta
    25921 tctcttacag aaatggtaaa gaaggcataa ctccccgaga aatactggct aagaagcctt
    25981 tatctggttg ttcttcttta ttgattaaag gaaggtcatc caaatcgccc agcaaatacg
    26041 tattttttgc ctgccagtca atcatccata ttttcagcag tggataagcc agaccgcccg
    26101 taatcccgga tatcaccacc aacgcacaca tgcgataaag cataccgtgg tacgttaaag
    26161 ttgagcgaaa acgaatacgc ccatcattca gtgacaggtt gctcataaaa tgatttcgca
    26221 acgacaccgt taaataactg gtgctaacag caatcccaaa ataatagatt aactgcgcga
    26281 ttatcatttt acgttgcatt tccataaatt gctgtaaatt ctcaatgtca tcatttgcat
    26341 atacagaact atcatacgca tttaatattt gatcgaagat aatataacca gcaacagcga
    26401 gaaaaggcaa taaagctaaa aatgcgagaa tggcatattt tatacaatac gtagtatcta
    26461 atttcacctt gaaacgatgt ataccgaaac tggtattgct ccagagaaag ctcattacca
    26521 ggctatataa agtaccatta aaaataccaa tggaaacaat accaactatt gccatcagaa
    26581 ccacagatat aataacacta cttgaactat tggcaggtag catctttgtc gagataaaga
    26641 aaacagtccc catcccaatg gccattaaaa tcggcaagaa aaaggttacc caccagaacc
    26701 ctttcaaaga gcaattaaaa ctaaatctta cgccgttgag actggtcatt aaggcctgat
    26761 aacgcagtcc ttttgctgcc ataaatataa gaaaaaccaa cagtaacaaa gtcaaaacac
    26821 agccaacaag cggcatatct gctgacactg tcattaagat tgcgaaatag aaaaaaacaa
    26881 aaacaagaca actaacaaaa acattcccac cggtaattcc ataagaaaat cgttgtccgt
    26941 taacttccat attagcataa agataacgct tacatttcat taatgcccat ggtaaataga
    27001 tacctagcgt tataatcgtt aacaacacat tcaccacaca aataagaaaa tatctctcac
    27061 ctgtaccatg aaaaacaaaa gaatgggacg gaacatccat ttcattaata acttgagcca
//
LOCUS       DAZKGF010000057        26442 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZKGF010000057 DAZKGF010000000
VERSION     DAZKGF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.057"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            257..1345
                     /gene="pdeL"
                     /locus_tag="ACHOQV_002232"
     CDS             257..1345
                     /gene="pdeL"
                     /locus_tag="ACHOQV_002232"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFP9630601.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(1387..2319)
                     /gene="yahB"
                     /locus_tag="ACHOQV_002233"
     CDS             complement(1387..2319)
                     /gene="yahB"
                     /locus_tag="ACHOQV_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9630602.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN"
     gene            complement(2411..2908)
                     /gene="yahC"
                     /locus_tag="ACHOQV_002234"
     CDS             complement(2411..2908)
                     /gene="yahC"
                     /locus_tag="ACHOQV_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP9630603.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYFGMVWAFFAALAAGWLASVSGISGFWASVIMTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            3166..3771
                     /gene="yahD"
                     /locus_tag="ACHOQV_002235"
     CDS             3166..3771
                     /gene="yahD"
                     /locus_tag="ACHOQV_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP9630604.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLNIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            3811..4674
                     /gene="yahE"
                     /locus_tag="ACHOQV_002236"
     CDS             3811..4674
                     /gene="yahE"
                     /locus_tag="ACHOQV_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP9630605.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMAAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYCGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAEKYKEEFYLGLQCGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            4664..6211
                     /gene="fdrA"
                     /locus_tag="ACHOQV_002237"
     CDS             4664..6211
                     /gene="fdrA"
                     /locus_tag="ACHOQV_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP9630606.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLRNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGMKQEHLDLHTLNQPLIADVRA
                     RMQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSVKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6211..7629
                     /gene="yahG"
                     /locus_tag="ACHOQV_002238"
     CDS             6211..7629
                     /gene="yahG"
                     /locus_tag="ACHOQV_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP9630607.1"
                     /translation="MSPSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPTAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            7655..7909
                     /locus_tag="ACHOQV_002239"
                     /pseudo
     CDS             7655..7909
                     /locus_tag="ACHOQV_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7959..8909
                     /gene="yahI"
                     /locus_tag="ACHOQV_002240"
     CDS             7959..8909
                     /gene="yahI"
                     /locus_tag="ACHOQV_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFP9630608.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGVFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPQRIVEAPAIKALIQQGFVVIGAGGGGIPVVCSEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            8919..10301
                     /gene="yahJ"
                     /locus_tag="ACHOQV_002241"
     CDS             8919..10301
                     /gene="yahJ"
                     /locus_tag="ACHOQV_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9630609.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTIHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENNQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMAAQQISI
                     ASTVQIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGNVAG"
     gene            10597..11007
                     /locus_tag="ACHOQV_002242"
     CDS             10597..11007
                     /locus_tag="ACHOQV_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9630610.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPLIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGCYSPEMDVIFEEYALIDVV"
     gene            11261..12244
                     /locus_tag="ACHOQV_002243"
     CDS             11261..12244
                     /locus_tag="ACHOQV_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9630611.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            12293..13777
                     /locus_tag="ACHOQV_002244"
     CDS             12293..13777
                     /locus_tag="ACHOQV_002244"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9630612.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLSFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYQNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            13770..14741
                     /locus_tag="ACHOQV_002245"
     CDS             13770..14741
                     /locus_tag="ACHOQV_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9630613.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKMGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            14738..15694
                     /locus_tag="ACHOQV_002246"
     CDS             14738..15694
                     /locus_tag="ACHOQV_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9630614.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGVNILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFTAVALGLWILLEKTPLGKSIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            15781..16830
                     /gene="yahK"
                     /locus_tag="ACHOQV_002247"
     CDS             15781..16830
                     /gene="yahK"
                     /locus_tag="ACHOQV_002247"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFP9630615.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDI
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEHLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSELKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            17452..17697
                     /gene="yahM"
                     /locus_tag="ACHOQV_002248"
     CDS             17452..17697
                     /gene="yahM"
                     /locus_tag="ACHOQV_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFP9630616.1"
                     /translation="MAVQLFKTLLNQIPLLSFLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(17714..18385)
                     /gene="yahN"
                     /locus_tag="ACHOQV_002249"
     CDS             complement(17714..18385)
                     /gene="yahN"
                     /locus_tag="ACHOQV_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFP9630617.1"
                     /translation="MMKLLHLFMVEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYIFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            18532..18807
                     /gene="yahO"
                     /locus_tag="ACHOQV_002250"
     CDS             18532..18807
                     /gene="yahO"
                     /locus_tag="ACHOQV_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFP9630618.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(18908..20494)
                     /gene="prpR"
                     /locus_tag="ACHOQV_002251"
     CDS             complement(18908..20494)
                     /gene="prpR"
                     /locus_tag="ACHOQV_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFP9630619.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGHEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFAAALRQGLQESETMLVHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            20733..21623
                     /gene="prpB"
                     /locus_tag="ACHOQV_002252"
     CDS             20733..21623
                     /gene="prpB"
                     /locus_tag="ACHOQV_002252"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFP9630620.1"
                     /translation="MYLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            21783..22952
                     /gene="prpC"
                     /locus_tag="ACHOQV_002253"
     CDS             21783..22952
                     /gene="prpC"
                     /locus_tag="ACHOQV_002253"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFP9630621.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            22986..24437
                     /gene="prpD"
                     /locus_tag="ACHOQV_002254"
     CDS             22986..24437
                     /gene="prpD"
                     /locus_tag="ACHOQV_002254"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFP9630622.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            24477..26363
                     /gene="prpE"
                     /locus_tag="ACHOQV_002255"
     CDS             24477..26363
                     /gene="prpE"
                     /locus_tag="ACHOQV_002255"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFP9630623.1"
                     /translation="MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVATRIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMAQV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
BASE COUNT         6486 a         6504 c         7163 g         6289 t
ORIGIN      
        1 caaaaatgag tatctgaatt ttaatattgc attcttgcgt gattatctcc tgagttttga
       61 cttgtgatta cctttttaag gtatttagcg taactgtttc tgagcgagca tcagaggtaa
      121 agataatctt cttgatagtg atgtgggatg ttatacgtat ggcatcgcta atgtttatgg
      181 ttacccctta tgtgtgctca ggaatccaca ggtaatcact catactgaac agcgataaaa
      241 gataaaaggt gtgttcatga attcatgtga ttttcgtgtt tttctgcaag agttcggtac
      301 aacggttcat ttgtcattgc ctggtagcgt atccgagaaa gaacgactgc tactcaagct
      361 gctgatgcag ggaatgtctg taacagaaat atcacagtac agaaatcgta gtgcaaagac
      421 catttcacat caaaagaaac agctctttga gaaactgggg attcagagcg atattacttt
      481 ctggcgggat attttctttc agtacaatcc ggagatcata tccgccacgg gtaataatag
      541 tcacaaatat attaatgata atcactatca ccatatcgtc acgcctgaag ccatcagtct
      601 ggcgttggaa aaccatgaat ttaaaccgtg gatccaaccg gttttctgcg cgcagactgg
      661 ggtactgacg ggctgtgagg tgcttgtccg ctgggaacat ccacaaacgg gaattatccc
      721 accggatcag tttattcctc tggcggagtc atccggtctt attgtcataa tgacccgcca
      781 gttgatgaaa cagactgcgg atattctgat gccggtaaaa catttgctgc cggacaattt
      841 ccatattggc atcaacgtct cggcgggttg ttttttggct gcgggatttg aaaaagagtg
      901 tctgaacctg gttaagaaat taggtaacga taaaatcaaa ctggttctcg agctaacgga
      961 acgtaaccct attccggtaa cgccagaagc cagagcgata tttgacagcc ttcatcagca
     1021 caacattacc tttgcgctgg atgactttgg tacgggttat gcgacctatc gttacttgca
     1081 ggcgttcccg gtcgatttta ttaagatcga taagtcattt gtgcaaatgg cgagcgtgga
     1141 cgaaatatcc ggtcatattg tggacaatat tgtcgaactg gcgcgtaagc ctggtctgag
     1201 tatcgtggcg gaaggggtag aaacccagga gcaggcggat ttaatgatcg gcaaaggcgt
     1261 tcactttttg cagggctatt tgtactctcc gccagtaccg ggtaataaat ttatctctga
     1321 atgggtaatg aaagcaggtg gttgatgtaa accgctattc acagcgcatc gggaggttgg
     1381 cagcgattaa ttctcccgat gcagtaaatc ctgatagatc cccgttaata tgccatttgg
     1441 cgaaaattgt tttttaatcc actgcgtcac ctgacccgta gcagaatgct gagttgcgag
     1501 taacatgcgt gaatcctggc gcggattatg gatttgtcgg gtaaccagca gggattgcgc
     1561 catcgcctca cggaccatgt aatccggcaa aaaaccaatt ccttcaccta aaatctggca
     1621 ctgacatttg gtgttgaaat ctggcaccag aatcgactcc tgcccatgca gcaaccagcc
     1681 cactttttta ttaatcgtat gcgcggtgtc ctccaccatg atattggggt aaagacgcag
     1741 ctggctttcg gcgatcggct ccggcacaaa ggctaatggg tgatccgggg cgatagcaaa
     1801 agcccagcga attgcgccaa tctccgtgta atcaatgccg ccgccgtcca gcagcgtatc
     1861 gggtgcgccg atagcaatat tcgcctgatt attaataatc gcatcccaga cgccgttata
     1921 cacttcggtc gtcacggtaa tctggcaggt tggaaactgc tttttcagta cctgtaataa
     1981 gcgtgcggtg tgtttgggcg tataaagcag ttggttaata caaatgcgca cccgtgcttc
     2041 tataccctgc gaaatagtat cgatgctgcg tttgatggcg tgaaaatcat tcagcaggtc
     2101 ggtagctttg cggaaaaaat aacgcccgga ctccgttaac tcaatgcttc gggtactgcg
     2161 agtgaacagc accacatcca gccctgtttc cattcgcttg atggtgtagc taatggcgga
     2221 agtggttaaa cccaactctt ctgcggcttt actgaaactg ccaaaacgcg cagcggtagt
     2281 aaatgccaac agattctctt cggtaaaaat tgagttcatt aaaccattcc cgccattatc
     2341 gaatatgaat ttctccagct atgactgtag aggtatcggt taaagataga taatcatttt
     2401 tgaataactt ttaatacccg tcgcgtttag tcgtcgcttc gcttaatttt tgtccgccat
     2461 attctgaaat tatccccacg cagttacccg ccagaaaacc cagtaatgtc accgtccagt
     2521 tcatcccgct ggcaaaaaat agcgtcatgc cgagaaagcc tccgggaata aaagacagta
     2581 aaccgaaacg tccttgccag accaccaccg ccgagaaggg tacagtcata atcacgctcg
     2641 cccagaaccc ggaaatccca cttacagagg ccagccagcc ggcagccagc gcggcgaaaa
     2701 aggcccacac catcccgaaa taattaaccg ccagacttct gataaacccc gatttaccgc
     2761 cgcctgcggc ataaaaactg caaaaggcga cgaagccaat ggttcctaat aattcccatc
     2821 cctgagagag cccgacatgt gaagacacta actgccaaag ccctgcacaa ataccgacgg
     2881 tgacacccgt tgccgtgagt ccgttcatct tgttcccctc tggttagaca tggacgaaag
     2941 ctactcttac atgtgcagac tatagagaaa taaaacctga ttgagtgaaa gggtatcggg
     3001 tcaaagaaac aaatattgca ctaccgcaca ctgcgaaaag attgttgaac atcattcaac
     3061 aaaaaggcga aatacggcga attaaattta agcgggttct tttatcagat tatcgttgct
     3121 attctcaatt catcgaaaac agccacaaaa accaggggaa tgtgaatgac tataaaaaat
     3181 ctacctgccg actatttatt ggctgcgcaa cagggtgata tcgataaagt aaaaacctgc
     3241 cttgcgctgg gtgtcgatat taatacctgc gatcgtcagg ggaaaacggc aattacgctg
     3301 gcaagtttat atcagcaata tgcttgcgtt caggcattaa ttgatgccgg agcggatatt
     3361 aataagcaag atcatacctg tttaaatcct tttttaatta gctgtctgaa cgatgattta
     3421 acgctactac gaattatttt accggctaaa cccgatctta attgcgtcac ccgttttggc
     3481 ggtgtcggcc tgacgcctgc ctgtgaaaaa ggccatttaa atattgtaaa agagcttttg
     3541 gcgcatacgg agattaacgt taaccagacc aaccatgttg gctggacgcc gctactggaa
     3601 gcgattgtgc ttaatgatgg cgggattaaa cagcaggcga ttgtccagtt attgctggaa
     3661 cacggtgcca gcccgcacat gacggataaa tatggcaaaa cgccgctgga actggcgcgg
     3721 gaacgaggct ttgaagagat tgcgcagtta ctgattgccg caggtgcata aaccgggagg
     3781 cttgctatca acacaccaga aagacggtgt gtgtgggcgc taactgcgga tgctgatttt
     3841 ctggcgcagc gggggcaagg acaggttgaa caggtctttg ccagagcggt aaatatcgca
     3901 cttccggccc gccagcagtt gctgacgctg ctttgtgaag agtacgacaa tgcgccaaac
     3961 agttgtcggt tggcactcac tcactttgat ggtctgttcc ggcatggtga taaggttcag
     4021 tttgacgatc aaggcattac ggttggtcaa catcttcata tagagatgag tcgttgtcgg
     4081 cgttggctgt ccccaacctt gcaaatggcc gctgtgaatt ttcaccttat cgcctggcaa
     4141 cagtggcatg acattattca tcagcacctg ggggaaaatg aaaccctgtt taattattgc
     4201 ggcgataatc cgttttatca ggcgttaaat aaagaattac atattaaacg acgggcagtt
     4261 attcaggccg taaacgataa acaaaatatc gcctcagcag tcgccagtat gatgggctta
     4321 gggattggcc ttacgccatc agccgacgat tatttaacag gtctggcgct tattttattt
     4381 atttccggac atccggcgga aaaatacaaa gaggaatttt atctcggtct gcaatgcggc
     4441 agaaataata ccacgttatt aagtgccata acgctggaag ccgcattaca acaacgctgc
     4501 cgggaaaata ttcatcgttt tattcataac attatttatg acatccctgg gaacgcaact
     4561 caggcaatag aaaaaattaa acatattggc tccagttccg gctgcgacat gctgtatggc
     4621 atggccgatg gttgtgcgct gagccaaacc tacggaggga attatgtcag ttaaaatagt
     4681 cattaaaccg aatacctatt ttgattctgt ctcgctgatg tctatctcca cgcgtgcaaa
     4741 taaactcgac ggcgtcgagc aggcatttgt ggcgatggcg accgaaatga acaaaggcgt
     4801 gctgaggaat ttaggactgc tgacgccgga actggagcag gcgaaaaacg gtgacctgat
     4861 gattgtcatc aatggtaaat cgggtgcgga caacgagcag ttgctggtgg agattgaaga
     4921 actgttcaac agcaaagcgc aaagtggctc gcacgaggcg cgttacgcca ctattgccag
     4981 cgccaaaaag catatcccgg aaagtaacct ggcggtgatt tcggtcaacg gtctgtttgc
     5041 cgctcgcgaa gcgcgtcagg cgctgcaaaa cgatcttaac gtgatgctgt tttccgataa
     5101 cgtctcagtt gaagatgaac tggcgctcaa acagttggcc cacgaaaaag ggctgctgat
     5161 gatggggcca gactgcggta cggcgattat caacggcgcg gcgctctgct ttggtaacgc
     5221 cgtgcgtcgc ggcaacatcg gcattgttgg cgcatccggc accggtagcc aggaactgag
     5281 cgtccgcatt catgaatttg gcggcggcgt ttcgcaactg atcggcaccg gcgggcggga
     5341 cctgagcgag aaaatcggcg gcctgatgat gctcgacgct atcgggatgc tggaaaacga
     5401 tccgcaaact gaaatcattg tgcttatctc caaaccgcca gcgcctgcgg tggcccgcaa
     5461 agtgctggaa cgcgcacgcg cctgccgtaa gccagtggta gtctgcttcc tcggtcgtgg
     5521 cgaaacgcca gtggatgagc aggggctaca gtttgctcgc ggcaccaaag aagcggcact
     5581 aaaagcggtg atgctctccg gtatgaaaca agaacatctc gacttgcata ccctgaacca
     5641 gccgttgatt gcggatgtgc gtgcgcgtat gcaaccgcag cagaaataca ttcgtggcct
     5701 gttctgcggc ggcacgctgt gtgacgaaac catgtttgcg gtgatggaaa aacatggcga
     5761 tgtctacagc aacattcagc ccgatcctga attccgcctg caagatatca accgcagcgt
     5821 caaacatacc ttcctcgact ttggcgatga cgattttacc aacggcaagc cgcatccaat
     5881 gattgacccg accaaccgca tcagccggtt gatcgaagaa gcgcgcgatc cagaagtggc
     5941 ggtgatcgtg atggattttg tgctcgggtt tggatcgcat gaagatccgg tcggttccac
     6001 catcgaggcg atcaaagagg cgaaagcgat cgccgctgcc gaagggcgcg agttgatcat
     6061 tctcgcctat gtgctgggta ccgatctcga tacgccatcg ttagaacaac aaagccagat
     6121 gttgcttgat gccggagtga ttctggccag cagcagcacc aataccggat tgctggcgcg
     6181 tgaatttatc tgcaaagggg aggaagcctg atgagcccgt cactgtttag ccaaccgttg
     6241 aacgttatta acgtcggtat cgccatgttt agcgatgatc tgaaaaagca gcatgtagaa
     6301 gtgacccaac tcgactggac gccgccgggg cagggcaata tgcaggtggt gcaggcgctg
     6361 gacaacattg ccgattcccc gctggcggac aaaatcgccg ccgctaacca gcaggcgctg
     6421 gagcgcatta tccagtcgca tccggtgctg attggttttg atcaggcgat taacgtggtg
     6481 ccgggtatga cgccgaaaac cattcttcac gccgggccgc cgatcacctg ggaaaaaatg
     6541 tgtggcgcga tgaaaggcgc ggtcaccgga gcgctggtgt tcgaagggct ggcgaaagat
     6601 ctcgatgagg cggctgaact ggctgcctcc ggggagatca ccttctcgcc gtgccacgag
     6661 cacgactgcg tgggttcgat ggcgggtgtt acctcggcct cgatgtttat gcacatcgta
     6721 aaaaacaaaa cctacggcaa catcgcttac accaatatga gcgagcagat ggcgaagatt
     6781 ttgcgtatgg gcgctaacga ccagagcgtg attgaccgcc tcaactggat gcgcgatgtg
     6841 caggggccca tactgcgcga cgcgatgaaa attatcggcg aaatcgatct gcgcttaatg
     6901 ctggcgcaag cgctgcatat gggcgatgag tgccataacc gcaataacgc cgggacgacg
     6961 ctgctgattc aggcgctgac gccagggatt attcaggcgg gctattccgt cgaacaacag
     7021 cgggaagtgt ttgagtttgt cgccagcagc gactacttct ccggcccgac gtggatggcg
     7081 atgtgtaaag cggcgatgga tgcggcgcac ggcattgaat acagcactgt ggtcaccacc
     7141 atggcgcgta acggcgtcga gttcggcctg cgggtcagcg gcctgccggg gcaatggttt
     7201 accggcccgg cgcagcaggt gatcggcccg atgtttgccg gttataagcc ggaagattcg
     7261 gggctggata tcggcgacag cgccatcact gaaacctacg gcattggcgg atttgccatg
     7321 gcgaccgcgc ctgcaatcgt tgcgctggtg ggcggcacgg tggaagaggc tatcgatttc
     7381 tcccgccaga tgcgtgaaat cactctcggt gaaaacccca acgtcaccat tccgctgctc
     7441 ggttttatgg gggtgccgac ggcaatcgac atcacccgcg tgggcagtag cggcattctg
     7501 ccggtgatca acaccgccat cgcccataaa gatgcgggcg tcggcatgat tggcgcgggc
     7561 attgtgcatc caccattcgc ctgcttcgag aaagccattc ttggctggtg cgaacgttac
     7621 ggcgtctgag ttacatgttt atgccggatg cgacatgaat gtcttatccg gcctacgaat
     7681 ggcgcggaaa tctgtaggcc tgataagcgt ggcgcttcag gcattggcca ccacagccgg
     7741 atgcgacgtg aacgccttat ccggcctacg aatggcacgg aaatctgtag gcctgataag
     7801 cgtagcgcat caggcattgg tcaccacagc cggatgcggc gtgaacgcct tatccggcct
     7861 acgagtggcg caagaatctg taggcctgat aagcgtagcg catcaggcag tctggcgttg
     7921 gtcataaccc catcaccctc tgtagcggac ataaaaatat gaaagaactt gtggtcgttg
     7981 ccattggtgg caacagcatt atcaaagata acgccagcca gtcgattgag catcaggcgg
     8041 aggcggtgaa agccgtcgcc gatacggtgc tggaaatgct ggcttccgat tacgacattg
     8101 tgctgaccca cggtaacggg ccgcaggtcg ggctggattt acgccgcgcg gagattgctc
     8161 acgagcgcga agggctgccc ttaacgccgc tggcaaactg tgtggcggat acgcaaggtg
     8221 gcattggcta tctgatccag caggcgctga acaaccggct ggcccgccac ggcgagaaaa
     8281 aagccgtcac cgtggtgact caggtggaag tggataaaaa cgatccggga tttgcccatc
     8341 ccaccaagcc tatcggcgta ttcttcagtg aaagccagcg tgacgaatta caaaaggcaa
     8401 accctgactg gcgttttgtt gaagatgccg gacggggcta tcgccgcgtg gtcgcctcgc
     8461 cggaaccgca acgtattgta gaagcacctg ccattaaggc cctgatccaa caaggctttg
     8521 tggtgattgg cgcgggcggc ggcggaattc ctgtggtgtg cagtgaagcg ggagattacc
     8581 aaagcgtgga cgcggttatc gacaaagatc tctctaccgc gctattggcc cgtgaaattc
     8641 acgccgacat tctggtaatt accaccggcg tcgaaaaagt gtgtattcac tttggcaaac
     8701 cgcagcagca ggcgctggat cgggtggata ttgccaccat gacccgctat atgcaggaag
     8761 ggcatttccc acccggcagc atgttgccaa aaatcatcgc cagcctgacg ttcctggagc
     8821 agggcggtaa agaagtgatt atcaccacgc cggaatgcct gcctgcggcg ctgcgtggtg
     8881 aaacgggcac ccatattatt aaaacgtaag gacgtaagat gaaagaaaac aatagccgcc
     8941 gtgagtttct gagccagagc ggtaagatgg tcaccgccgc cgcgctgttt ggtacctctg
     9001 tgccgctcgc ccatgcggca gtctctggca caatccattg cgacgcgaat aacaccatga
     9061 aaatcactga cccgcactac tatctcgaca acgtgttgct ggaaaccggt tttgactacg
     9121 aaaatggcgt ggcggtgcag acccgcacgg cgcgccagac tgtggaaatt cagaacggca
     9181 aaattgttgc cctgcgcgag aacaatcagc atccggacgc cacgctgccg cactatgacg
     9241 ctggcggtaa gctgatgctg cccaccaccc gcgacatgca tattcatctc gacaaaacct
     9301 tctatggcgg gccgtggcgc tcgctcaatc gtccggcagg caccaccatc caggacatga
     9361 tcaaactcga gcagaaaatg ctgccggagt tgcaaccgta cactcaggaa agggcggaaa
     9421 aactgattga tttattgcag tcgaaaggca ctaccattgc ccgcagccac tgcaatatcg
     9481 aaccggtttc cggcctgaaa aatctgcaaa atttgcaggc ggttctggcg cgacgtcagg
     9541 cgggcttcga gtgtgaaatt gtcgccttcc cgcagcacgg tttgctgctg tcgaaatcag
     9601 aacccttaat gcgcgaagcg atgcaggcgg gggcgcatta cgtcggcggg ctggacccga
     9661 ccagtgttga tggcgcgatg gaaaaatccc tcgacaccat gttccagatt gcgctggact
     9721 acgacaaagg cgtcgatatt cacctgcacg aaaccactcc ggcaggcgtg gcagccatca
     9781 attatatggt tgaaacggta gagaaaacgc cacagctgaa gggcaagctg accatcagcc
     9841 acgccttcgc gttggcaacg ctcaacgaac aacaggtaga tgaactggcg aaccggatgg
     9901 cggcgcagca aatttctatc gcctcgacgg tgcagatcgg cacgctacat atgccgctta
     9961 aacaactgca cgacaaaggc gtaaaagtca tgaccggaac tgacagcgtt attgaccact
    10021 ggtcgcctta cggcctgggc gacatgctgg aaaaagccaa tctctacgcg cagctctata
    10081 ttcgtcctaa cgaacagaac ctctcccgtt cgctgttttt agctactggc gatatattgc
    10141 cgctcaacga aaaaggcgag cgtgtgtggc ctaaagctca ggatgacgcc agctttgtgc
    10201 tggtggacgc ctcctgttcc gccgaggcgg tggcgcgtat ctcgccgaga accgcaacgt
    10261 tccataaagg gcaactggtg tgggggaatg tggcaggttg atgcgcattt gatatgtctg
    10321 caacggtgaa cagtaagaga tttaagcccc agggagtggg gcgggaatgg tttgttaatg
    10381 aatgatcctg aactttataa tctcccggca taatttccag gggattattt gagagaaata
    10441 cacaaaagtg agttttaaca aaggtattta tcaagccata aatgtgacct gcttcggata
    10501 aaaaagatta aacataggaa aaccaatgat actcttgctg atggttacca acagtaatta
    10561 agatgtgatt ttttccgtaa tcgaaaaaat attgccatga ccataaaaga aaaattctca
    10621 caaaaatatc cccatgcttc cttttgtact tttggcgatt cagcagcgtt ggccgatcac
    10681 ctcgcaacgt tgattgcgac tggtgttaaa acagcttcct gtggttcgct ggcgggatgt
    10741 attgaagaca acgcatttcc gctgattggt gagtataaga ttgtagaaaa tagccgtggt
    10801 gagcctgtct gtgttatcag agtgattggc ctgcatttac tgcgcttttc tgatgtcacc
    10861 gcagagcttg cccgtaagga aggtgaaggt gacctcagtc ttgaatattg gcgtaatgaa
    10921 caccgtcgtt ttttccaggc agaaggatgc tattcaccag aaatggacgt tatctttgaa
    10981 gaatatgccc ttattgacgt tgtgtagata actatttttc tacgcgaaat aagctcacaa
    11041 acataaagta tcgctaattt tgtttatttt gctgtttttt acgtgatgat tgtcacactt
    11101 attgttgctg cccgttttgt ccatatttat aaccaaattt ttaatagctg cctgaaatag
    11161 ggctgtttat tctgcgtaaa aatattcgaa aattcttatt ctctccagcg cgctaatttg
    11221 ggcgaacact tcctgactac cctgcaatga ggctgaaatg atgaataaac gttttgttat
    11281 caatatggtg tcttcgctgt tattaggcgc tgcgctaatt tctgcgccgc tccaggcagc
    11341 ggaaaaagtc gtggtgaata tctctaaagt cgatggtatg ccgtggttta atcgcatggg
    11401 cgaaggtgtg gttgaggcgg gtaaagcctt tggtgttaat gcgtcacagg tggggccttc
    11461 aagtactgat gctccgcaac aggttaaaat tattgaggat ttaatcgccc gtaaggttaa
    11521 tgcaattact atcgtgccaa acgatgccaa cgtgctggaa ccggtattca aaaaagcgcg
    11581 agatgccgga attgtggttc tgaccaacga atctcccggt cagccaagtg ctaactggga
    11641 tatcgaaatc attgataacg aaaaatttgc cgctgaatat gttgagcata tggcgaaacg
    11701 catggggggc aagggcggct atgtgattta cgtgggtagt ttgaccgtgc cgcagcataa
    11761 cttatgggct gacttgctgg tgaaatacca gaaagagcat tacccggata tgcatgaagt
    11821 tacccgccgc atgccggttg ccgagagtgt ggatgactca cgtcgtacca cactggacct
    11881 gatgaaaacc tatcctgact tgaaagcggt tgtctccttt ggttcaaacg gtccgattgg
    11941 cgcgggacgt gcagtgaaag agaaacgggc gaaaaataaa gtcgcagtct atggcatgat
    12001 gatcccttca caggcggcat cactgattaa aagtggtgat atcaccgaag ggattactta
    12061 cgatccggcc tctgcaggtt acgcactcgc ggccgtcgcc agcacgttgc tgaagggtga
    12121 agagatcaaa cctggattag aaatgcagaa tctcggtaag gccgacgtag atatggataa
    12181 gcgaattatc cgtttccata aggtattact ggtcaacaaa gacaatatcg actcacttta
    12241 ttaatccatt tcttcggggc gaaagccccg attcttattg ccaggggatg ctatggaaac
    12301 cttcctttcc cttcgtcata tcaacaaaac gtttcatgcc acgcgagcgc tgcgcgatgt
    12361 ttctctcgac tttatgtcgg gtgaggtgca ctgccttgcc gggcaaaacg gctgtggtaa
    12421 atcgacgttg attaaaatta tgtccggtgt ttatcgcccg gatgaggggg cggaaattac
    12481 gcttggtggg aaaaactggt caaagctgac acccgccgct tcggtggcgc aggggattca
    12541 ggtgatttat caggacctct ctttatttcc taacctgagt gtctgggaga atatcgccgt
    12601 gaatcactat caccacggtc tgtttgttaa ccgccgtcgt ctgcgtgagg tggcgcaggc
    12661 ggcaatgacc agtattaacg tcacattgcc actggatacg ctcgtttctg agctttcaat
    12721 tgcccgctgt cagctggtgg caatttgccg tgcactggcg caggacgcac ggttgatcgt
    12781 gatggatgaa ccaaccgctt cgctgaccca tcaggaagtg caagggttat tacaggtggt
    12841 gcatcaattg cgggaacgcg ggatctgcgt ggtcttcgtc agtcatcgtc tggaagaagt
    12901 gatggaagtt tccgaccgta tttcagtgct gaaagatggt gagctggtcg ggacctttcc
    12961 ggccgcagag atgaccacaa aacaactcag ttttctgatg accggccagg agttcgaata
    13021 tcaggtacga gagttgtggc agggaaaatc cagcaccccg gtgctggagg tgcgtaattt
    13081 aagtcgtcat ggggaatatc aaaatattaa cttgcgggtg gaagcgggcg aagtggtgtc
    13141 gattgttggc ctgcttggtg cggggcgcac ggaattatgt ctgagtctgt ttggtatgac
    13201 ccgaccggat gctggtgaga tcctcatcaa cggtcagccg gttacactgc atagtaacca
    13261 ggacgccatc cgccacggta ttggttatgt ttcggaagat cgcatgtcgc gcggtttggt
    13321 gatggcgcaa tctattgaag acaacatcat tagcactgtt tttcacaagg taaaagatcg
    13381 cttcggcttt ctgagtgaag ctaaagtgcg tgacctggtg gacaggctaa tcaaggcatt
    13441 gaccatcaaa gtctctgacc cacatctccc ggtgaatacg ctttccgggg gtaacgcgca
    13501 gcgagtatcg atcgccaaat ggctggcgat tgggccacga ctgttgattc tcgatagccc
    13561 gactgtcggt gttgatatcg ccaacaaagc aggaatatac ggcattatca gcgatctggc
    13621 ggctcatggc attgcggtat tgatgatctg cgatgaaatt gaagaagcgt ggtatcaaag
    13681 ccaccggatt ttggtaatgc aaaaaggtca gatcacccat agcttcctgc ctgacagcag
    13741 ttctcaggcc aggattgcgg aggtggtaaa tggctgaact gaaaaaacgc catgaattct
    13801 ggctggcatt actgattgtg gtgctgtttg ttggccttgc ctggcgcagc gacgagtttc
    13861 tgacattcgg taatttgtac gatctcgcca ataactatgc catgttgact attctcgcct
    13921 gtggtttgtt tgtggtgtta atttccggtg ggattgatat ttcgtttccg gcaatgacca
    13981 tcattgcgca atacggcatg gtgctgttgc tgcaaaaaat gggtggcaac tttgctgtcg
    14041 cgtttgccct ggcgggcggc atcggcattt tacttggctt aataaacgcc ttactggtta
    14101 atcgcctacg ggtgccgtct atcatcatca ctatctcgac gctgaatatt ttttatggcc
    14161 tgctgttatg gttgagtaaa ggtgtgtggc tgtacgactt tccgccgtgg tttgagcagg
    14221 gggtaatgtt gttcaagtac accgatgctg atggctatga ctatggcctt ggtctgccgc
    14281 tgatcgccat gattacggtg gtgctgctga cagcgtttat catgaatttc accagtgtag
    14341 ggcgcaaaat ttatgccctt ggcgggaacc gcgaatcagc cagtcgcatc ggctttagtg
    14401 tgctgaaact gcaacttttc gtctatggct atatgggatt gatgtctggt gctgcgggtg
    14461 tagtgcagtc gtggacggtg atgactgtcg cccccgattc tcttctgggt tatgagctga
    14521 cagtactggc tgcggtggtg cttggtggca ctagtttgct cggcgggcgc ggcacgttaa
    14581 ccggtacttt gctcggcgtg gtgttgttgg cagtaatgca aaacgggcta aatttattgg
    14641 gagtctcgtc ttactggcaa acattgatca caggcatcat catcgttgcc agcattagcg
    14701 ccacggcgtg gagtcagcat cagaaccgga gtctgctatg aaaaaatcgt ggcgtaataa
    14761 cgttgagttt tacttgatcg gcctgctggt gctaactgtt gccgcgttca gcattactat
    14821 gccggagatt ttctggtcga taagtaattt ccagtccgtc gcttcgcaaa tgcccgtgct
    14881 gggcattctg gcgttggcta tggcggtgac catgctttgc ggtggcatta acttgtcaat
    14941 tatagccact gctaacgcct gttcactggt gatggcgtgg gtggcgactc agtatccacc
    15001 gggtattgct acggttgtcg ctacgctgct ggcgggcgct ggcgcggcgg tgataatcgg
    15061 tttatgtaac ggcgtattga ttgctggtat tcgagtgtcg ccaattcttg caacgcttgg
    15121 gatgatgacg ttgctcaaag gggtaaatat tctggtgaca ggcggtagcg ccattgccaa
    15181 ttatccctca tgggtgctgt ggttgaatca tgcacagtgg ttcggtattc cgctaccgat
    15241 gtggttgttt accgcagtgg cgctggggct gtggatattg ctggagaaaa cgccgctcgg
    15301 taaatccatt tatctgattg gttctaacga acgagcaaca ctttactccg gtattaacac
    15361 tcgtcgggtg ctgatatggg tgtatgtcat ttccgccttg ctttgtgcgg tagcggcatt
    15421 tttgatgatg tcgaaactca actcggctaa ggcctcttat ggcgaatctt acttgctagt
    15481 gtcaatcctc gcggcagtac tcggcggggt taatccggac ggcggcagcg ggcggataat
    15541 tggtatggtg ctggcgctgt ttcttctgca aattattgaa agcggcttca atattctcgg
    15601 tataagtccc tacctgacga tggccttatg gggaacgttg ctgctctgtt tcattcaggc
    15661 tcgcggcatg ttggggctgg atcgggtggt ttaaatattg gctatattca atggacgcgt
    15721 tttgccgcga tgacatatca ggcatcgcca aatacacata gctaatcagg agtaaacaca
    15781 atgaagatca aagctgttgg tgcatattca gctaaacaac cacttgaacc gatggatatc
    15841 acccggcgtg aaccgggacc gcatgatgtc aaaattgaaa tcgcttactg tggcgtctgc
    15901 cattccgata tccaccaggt ccgttccgag tgggcgggga cggtttatcc ctgcgtgccg
    15961 ggccatgaaa ttgtaggacg cgtggtagct gttggtgatc aggtagaaaa atatgcgccg
    16021 ggcgatctgg ttggtgtcgg ctgcattgtc gacagttgta aacattgcga agagtgtgaa
    16081 gacgggctgg aaaactactg tgatcacatg accggcacct ataactcgcc gacgccggac
    16141 gaaccgggcc atactctggg cggctactca caacagatcg tcgttcatga gcgatatgtt
    16201 ctgcgtattc gtcacccgca agagcacctg gcggcggtgg ctcctttgtt gtgtgcaggg
    16261 atcaccacat actcgccgct acgtcactgg caggccgggc cgggtaaaaa agtgggcgtg
    16321 gtcggcatcg gcggtctggg acatatgggg atcaagctgg cccacgcgat gggggcgcat
    16381 gtggtggcat ttaccacttc tgagttaaaa cgcgaagcgg caaaagccct gggggccgat
    16441 gaagttgtta actcacgcaa tgccgatgag atggcggctc atctgaagag tttcgatttc
    16501 attttgaata cagtagctgc gccacataat ctcgacgatt ttaccacctt gctgaagcgc
    16561 gatggcacca tgacgttggt tggtgcgcct gcgacaccgc ataaatcgcc ggaagttttc
    16621 aacctgatca tgaaacgccg cgcgatagcc ggctctatga ttggcggcat tccagaaaca
    16681 caggagatgc tcgatttttg cgccgaacat ggcatcgtgg ctgatataga gatgattcgg
    16741 gccgatcaaa ttaatgaagc ctatgagcga atgctgcgtg gtgatgtgaa atatcgtttt
    16801 gttatcgata atcgcacact aacagactga aaaaattaat aaacatcctg tggtttaaca
    16861 tattaacttc gctccactta actttttagt taaggagagc gaaataatat caaagtagtg
    16921 gtaaacctat agcgtaaatt cagattgtta aattaactcc actactttac acgttagtta
    16981 tgagcgattt ctgatagtgt ctggtttaat cagagcttta ttatctgcga cgtttatttt
    17041 tatttaatag agccacctgc aagttgttat tccaccagag agtgaatcgc aaagcaggta
    17101 atcatttcat ctgtatatct gtatgccaac tggcattcca tctctttttt cgccgatatt
    17161 ttgttgtgat cggcgacact tcgcttaaaa aagcatcagc agtggtttcg cagctatgcg
    17221 gtgtataaaa aatgatctca tgcagatgtt ttgtgaatgt gtgggttgac attcatatga
    17281 aaaaaatcat aattccatca tgtttgtgtg gggtcttttc tgtatcttac gcatcgcact
    17341 caagcctgac agaaaatatg ctgtaaggct catatcaaaa accgccatta gctcatcagg
    17401 aagagcagac gacaaccagg attgttgtat ggtacggggt tcgaggcctc gatggcagtc
    17461 cagcttttca aaactcttct gaatcagatc cccttattat cattcctgca aagtggaaca
    17521 ttgccattgt ttggctattc aggacgggtc agaccaatga aaaaagccca cacaggggag
    17581 agtgggctga aatgggaagc taaagactca agtaaactta tcggaaataa ggaccacgca
    17641 ttacgggggc tatcatcgcc gatggtcggg attcggcaaa tcaggttgat tacgtagaga
    17701 gtaaattatc tgctcaccgc tgcgtcaccc cttcgtaaat caggcgtagc gcgaatacac
    17761 caataattgc accaataacc cgactggcaa cgcgttgcat acgcccataa gcacgacgca
    17821 ctgcgggcaa agaaaacgcc tgactaagaa aaacgcgcca gataactgat gcgagcacaa
    17881 tccccgccca ggccattaaa cgtgcccatg ttggtgtttc ggcatttaat gttactgaga
    17941 aaatactaat aaaaaataaa acggtttgcg ggttagagag atcggtaatt aatccacggc
    18001 ggaaaaagat ataccagggg gcactaatcg gttgttgtag tgtgctcatt tgcggtgttg
    18061 actggcggcg catgctgcac cacgcaaacc ataagagata agcgccgccg acgattctga
    18121 taagcgaaaa aatctcctca cactgcgtaa ttagtgttgc aagaccaaac aaacccaacc
    18181 cggaataaaa tgcatcgccc aacgccacgc ccagtccggt cagcacccct gcgcgtcgac
    18241 cggaagccag gctggtttgt actaccacaa agagattggc tcccggatta aaaaaagtaa
    18301 tcacgaacag tcctacggtc agataaacgg cgtgcaaagg atccatagtg atttcaacca
    18361 taaataagtg aagtaacttc atcatatttc taccaaaaat aatcgggtgc gagagagatc
    18421 acaaagtgtc ttatttccgg ttactggcgt ttatgccctg actgaactaa ttattaatca
    18481 acccaataat gtgggtgggt gatagtgtga taacaactct ggagccgtaa tatgaaaata
    18541 atctctaaaa tgttagtcgg tgcgttagcg tttgccgtta ccaatgtcta tgccgcagaa
    18601 ttaatgacca aagcggaatt tgaaaaggtt gcatcgcaat atgaaaaaat aggtgatatt
    18661 tcaaccagta atgaaatgtc gaccgcagat gcgaaagaag atttgatcaa aaaagccgat
    18721 gaaaaagggg ctgatgtgtt ggtactgacc tctggtcaaa ctgacaataa gatccacggc
    18781 acggcaaata tttataagaa gaagtaattc tgaatcctat gtaaacatcc ccgatgcgta
    18841 agtttatcgg tgatcatcat ctattgaaat ttatgccgga taaagcgttc gcgctgcatt
    18901 cggcagttca gcttttcagc cgccgccaga acgtcgtccg gctgatacct aaataattcg
    18961 ccgctgctgt tttatcgcca ttaaatttct ccagtgcctg ttgtgctgtc agcaagcctg
    19021 gaatgggagt cttcgccgac tcgcgtgcca gttccggcag tagcagctgc aaaaattgcg
    19081 gcgttaaatc cggcgtcgat tccacactta aaaacagcgc cagtcgttcc atcatattgc
    19141 gcagttcacg aatattgccc ggccagtcgt agtgcaccag catggtttcg ctttcctgta
    19201 atccctggcg taatgcggca gcaaacgggg cggagagcgc cgccagagac actttcaaaa
    19261 aactttccgc cagcggcaga atatccgcca cccgctcgcg cagtggtggc aattgcagac
    19321 gcaaaatact cagccgataa aacagatcac ggcgaaactg cccttgccgc atatcttctt
    19381 ccagattgca gtgagtggcg ctaatgactc gcacatccac cggaacaggc tgatgcccgc
    19441 cgacgcgggt gacctctttt tcttccagca cccgcagcag ccgagtctgc aacggcagcg
    19501 gcatttcgcc tatctcgtca agaaacagcg tgccaccgtg ggcgatttcg aacagcccgg
    19561 cgcgacctcc gcgtcgcgag ccggtaaacg ccccttcctc atggccaaac agctccgctt
    19621 ccagcagcga ttcggcaatc gctccgcagt tgacggcaac aaacggatgc gactttttgc
    19681 cctgtcgcgc atcgtggcgg gcaaaatatt cccgatgaat cgcctgggcc gccagctctt
    19741 tgcccgtccc cgtttccccc tcaatcaaca ccgccgcact ggagcgggca tacagcaaaa
    19801 tagtctgccg cacctgttcc atctgtggtg attgaccgag catatcgccc agcacgtaac
    19861 gagtacgcag ggcgttgcgg gtggcatcgt gagtgttatg gcgtaacgac atgcgcgtca
    19921 tatccagcgc atcgctgaat gcctggcgca cggtggcggc ggaatagata aaaattccgg
    19981 tcattccggc ttcttctgcc agatcggtaa tcagccccgc gccgaccacc gcttcggtgc
    20041 cgttagcttt cagctcgtta atctgcccgc gcgcgtcttc ttcggtaatg tagctacgtt
    20101 gatcgaggcg caaattaaag gttttttgaa acgccaccag tgccggaata gtttcctgat
    20161 aagtgacaac gccgatagaa gaggtgagct ttccggcttt tgccagagcc tgtaacacat
    20221 cgtagccgct cggtttaatc aaaataaccg gcactgacag gcgactttta agatatgcgc
    20281 cgttagagcc agcagcgatg atggcgtcgc agcgttcgtt tgccagtttt ttgcggatgt
    20341 aggtcaccgc tttttcaaag ccaagctgaa taggggtgat attcgccagg tgatcaaact
    20401 cgagactgat atcgcgaaac agctcgaaca gccgcgttac agataccgtc cagatcaccg
    20461 gtttgtcgtc attcagccgt ggtggatgtg ccatagcgca ccgcaaagtt aagaaaccga
    20521 atattgggtt tagtcttgtt tcataattgt tgcaatgaaa cgcggtgaaa cattgcctga
    20581 aacgttaact gaaacgcata tttgcgaatt acttcatgac tatattgcta acaaattgaa
    20641 attaaacatt taattttatt cagtcaattg tggcacaccc cttgctttgt gtttatcaac
    20701 gcaaataaca agttgataac aaaggatggg ctatgtatct acactctcca ggtaaagcgt
    20761 ttcgcgctgc actgtctaaa gaaaatccat tgcagattgt tggcaccatc aacgctaacc
    20821 atgcgctgct ggcgcagcgt gccggatatc aggcaattta tctctccggc ggtggcgtgg
    20881 cggcagggtc gctggggctg cccgatctcg gtatttctac tcttgatgac gtgctgacag
    20941 atattcgccg tatcaccgac gtttgttcgc tgccgctgct ggtggatgcg gatatcggtt
    21001 ttggttcttc tgcttttaac gtggcgcgca ccgtgaaatc gatgattaaa gccggtgcgg
    21061 caggattgca tattgaagat caggttggtg cgaaacgctg cggtcatcgt ccgaataaag
    21121 cgatcgtctc gaaagaagag atggtggatc ggatccgcgc ggcggtggat gcgaaaaccg
    21181 atcctgattt tgtgatcatg gcgcgcaccg atgcgctggc ggtagagggg ctggatgcgg
    21241 cgattgagcg tgcgcaggcc tatgttgaag cgggtgccga gatgttgttc ccggaggcaa
    21301 ttaccgaact cgccatgtac cgccagtttg ccgatgcggt gcaggtgccg atcctcgcca
    21361 acatcaccga atttggcgca acaccgctgt ttaccaccga cgaattacgc agcgcccatg
    21421 tcgcaatggc gctgtaccca ctttcagcgt tccgcgccat gaaccgcgcc gctgagcatg
    21481 tctacaacgt cctgcgtcag gaagggacgc agaaaagcgt catcgacacc atgcagaccc
    21541 gcaacgagct gtacgaaagc atcaactact accagtacga agagaagctc gacgacctgt
    21601 ttgcccgtgg tcaggtgaaa taaaaacgcc cgttggttgt attcgacaac cgatgcctga
    21661 tgcgccgctg acgcgactta tcaggcctac gaggtgaact gaactgtagg tcggataaga
    21721 cgcatagcgt cgcatccgac aacaatctcg accctacaaa tgataacaat gacgaggata
    21781 atatgagcga cacaacgatc ctgcaaaaca gtacccatgt cattaaaccg aaaaaatcgg
    21841 tggcgctttc tggcgttccg gcgggcaata cggcgctctg caccgtgggt aaaagcggca
    21901 atgatttgca ttaccgcggc tacgacattc ttgatctggc ggaacattgc gaatttgaag
    21961 aagtggcgca tctgctgatc cacggcaaac tgccgacccg tgacgaactc gccgcctaca
    22021 aaacgaaact gaaagccctg cgcggtttac cggctaacgt gcgtaccgtg ctggaagcct
    22081 taccggcggc gtcacacccg atggatgtta tgcgcaccgg cgtttccgcg ctcggctgca
    22141 cgctgccaga aaaagagggg cataccgtct ctggcgcgcg ggatattgcc gacaaactgc
    22201 tggcgtcact tagctcgatt cttctctact ggtatcacta cagccacaac ggcgaacgca
    22261 ttcagccgga aaccgatgac gactccatcg gcggtcactt cctgcatctg ctgcacggcg
    22321 aaaagccgtc gcaaagctgg gaaaaggcga tgcatatttc gctggtgctg tacgccgaac
    22381 acgagtttaa cgcctccacc tttaccagcc gggtgattgc gggcaccggc tctgatatgt
    22441 attccgcgat tattggcgcg attggtgcac tgcgcgggcc gaagcacggc ggggcgaatg
    22501 aagtgtcgct ggagatccag caacgctacg aaacgccgga cgaagccgaa gccgatatcc
    22561 gcaagcgggt ggaaaacaaa gaagtggtca ttggttttgg tcatccggtt tataccatcg
    22621 ccgacccgcg tcatcaggtg atcaaacgtg tggcgaagca gctctcgcag gaaggcggct
    22681 cgctgaagat gtacaacatc gccgatcgcc tggaaacggt gatgtgggag agcaaaaaga
    22741 tgttccccaa tctcgactgg ttctccgctg tttcctacaa catgatgggt gttcccaccg
    22801 agatgttcac accactgttt gttatcgccc gcgtcactgg ctgggcggcg cacattatcg
    22861 aacaacgtca ggacaacaaa attatccgtc cttccgccaa ttatgttgga ccggaagacc
    22921 gccagtttgt cgcgctggat aagcgccagt aaacctctac gaataacaat aaggaaacgt
    22981 acccaatgtc agctcaaatc aacaacatcc gcccggaatt tgatcgtgaa atcgttgata
    23041 tcgtcgatta cgtgatgaac tacgaaatca gctccaaagt agcctacgac accgcacatt
    23101 actgcctgct cgacacgctc ggctgcggtc ttgaagctct cgaataccca gcctgtaaaa
    23161 aactgctggg gccaattgtc cccggcaccg tcgtacctaa cggcgtgcgc gtccccggaa
    23221 ctcagttcca gctcgacccc gtccaggcgg catttaacat cggcgcgatg atccgttggc
    23281 tcgatttcaa cgatacctgg ctggcggcgg agtggggcca tccttccgac aacctcggcg
    23341 gcattctggc aacggcagac tggctttcgc gcaacgcggt cgctagcggc aaagcgccgt
    23401 tgaccatgaa gcaggtgctg accgcaatga tcaaagccca tgaaattcag ggctgcatcg
    23461 cgctggaaaa ctcctttaac cgcgtcggcc tcgaccacgt tctgttagtg aaagtggctt
    23521 ccaccgccgt ggtcgccgaa atgctcggcc tgacccgcga ggaaattctc aacgccgttt
    23581 cgctggcgtg ggtggacggt cagtcgctgc gcacctatcg ccatgcgccg aacaccggca
    23641 cgcgtaaatc ctgggcggcg ggcgatgcca cttcccgcgc ggtacgtctg gcactgatgg
    23701 cgaaaacggg cgaaatgggt tatccgtcag ccctgactgc gccagtatgg ggtttctacg
    23761 acgtctcctt taaaggtgag tcattccgct tccagcgtcc gtacggttcc tacgtcatgg
    23821 aaaatgtgct gttcaaaatc tccttcccgg cggagttcca ctcccagacg gcagttgaag
    23881 cggcgatgac gctctatgaa cagatgcagg cagcaggcaa aacggcggca gatatcgaaa
    23941 aagtgaccat tcgcacccac gaagcctgta ttcgcatcat cgacaaaaaa gggccgctca
    24001 ataacccggc agaccgcgac cactgcattc agtacatggt ggcgatcccg ctgctgttcg
    24061 gacgcttaac ggcggcagat tacgaggaca acgttgcgca agataaacgc atcgacgccc
    24121 tgcgcgagaa gatcaattgc tttgaagatc cggcgtttac cgctgactac cacgacccgg
    24181 aaaaacgcgc catcgccaat gccataaccc ttgagttcac cgacggcaca cgatttgaag
    24241 aagtggtggt ggagtaccca attggtcatg ctcgccgccg tcaggatggc attccgaagc
    24301 tggtcgataa attcaaaatc aatctcgcgc gccagttccc gactcgccag cagcagcgca
    24361 ttctggaggt ttctctcgac agaactcgcc tggaacagat gccggtcaat gagtatctcg
    24421 acctgtacgt catttaagta aacggcggta aggcgtaagt tcaacaggag agcattatgt
    24481 cttttagcga attttatcag cgttcgatta acgaaccgga gaagttctgg gccgagcagg
    24541 cccggcgtat tgactggcag acgcccttta cgcaaacgct cgaccacagc aacccgccgt
    24601 ttgcccgttg gttttgtgaa ggccgaacca acttgtgtca caacgctatc gatcgctggc
    24661 tggagaaaca gccagaggcg ctggcattga ttgccgtctc ttcggaaaca gaggaagagc
    24721 gtacctttac cttccgccag ttacatgacg aagtgaatgc ggtggcgtca atgctgcgct
    24781 cactgggcgt gcagcgtggc gatcgggtgc tggtgtatat gccgatgatt gccgaagcgc
    24841 atattaccct gctggcctgc gcgcgcattg gtgctattca ctcggtggtg tttgggggat
    24901 ttgcttcgca cagcgtggca acgcgaattg atgacgctaa accggtgctg attgtctcgg
    24961 ctgatgccgg ggcgcgcggc ggtaaaatca ttccctataa aaaattgctc gacgatgcga
    25021 taagtcaggc acagcatcag ccgcgtcacg ttttactggt ggatcgcggg ctggcgaaaa
    25081 tggcacgcgt cagcgggcgg gatgtcgatt tcgcgtcgtt gcgccatcaa cacatcggcg
    25141 cgcgggtacc ggtggcgtgg ctggaatcca acgaaacctc ctgcattctc tacacctccg
    25201 gcacgaccgg caaacctaaa ggcgtgcaac gtgacgttgg cggatatgcg gtggcgctgg
    25261 cgacctcgat ggacaccatt tttggcggca aagcgggcgg tgtgttcttt tgtgcttcgg
    25321 atatcggctg ggtggtgggg cattcgtata tcgtctacgc gccgctgctg gcggggatgg
    25381 cgactatcgt ttacgaagga ttaccgacct ggccggactg cggcgtgtgg tggaaaatcg
    25441 tcgagaaata tcaggttagc cgcatgttct cagcgccgac cgccattcgc gtgctgaaaa
    25501 aattccctac cgctgaaatt cgcaaacacg atctctcgtc gctggaagtg ctctatctgg
    25561 cgggggagcc gctggacgag ccaaccgcca gttgggtgag caatacgctg gatgtgccgg
    25621 tgatcgacaa ttactggcag accgaatccg gctggccgat tatggcgatt gctcgcggtc
    25681 tggacgacag gccgacgcgt ctgggaagcc ccggcgtgcc gatgtatggc tataacgtgc
    25741 agttgctcaa tgaagtcacc ggcgaaccgt gcggcgtcaa cgagaagggg atgctggtgg
    25801 tggaagggcc gctgccgccg gggtgtattc agaccatctg gggcgatgac gaccgctttg
    25861 tgaagacgta ctggtcgctg ttttcccgtc cggtgtatgc cacctttgac tggggcatcc
    25921 gcgatgctga cggttatcac tttattctcg ggcgcactga cgatgtgatt aacgttgccg
    25981 ggcatcggct ggggacgcgc gagattgaag agagtatctc cagtcatccg ggcgttgccg
    26041 aagtggcggt ggttggggtg aaagatgcgc tgaaagggca ggtggcggtg gcgtttgtca
    26101 ttccgaaaga gagcgacagt ctggaagacc gcgaggtggc gcactcgcaa gagaaggcga
    26161 ttatggcgca ggtggacagc cagattggca actttggccg cccggcgcac gtctggtttg
    26221 tctcgcaatt gccgaaaacg cgatccggaa aaatgctgcg ccgcacgatc caggcgattt
    26281 gcgaaggacg cgatcctggg gatctgacga ccattgatga tccggcgtcg ttggatcaga
    26341 tccgccaggc gatggaagag taggtcggat aaggcgctcg cgccgcatcc gacaccgtgc
    26401 gcagatgcct gatgcgacgc tgacgcgtct tatcatgcct ac
//
LOCUS       DAZKGF010000058        26432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZKGF010000058 DAZKGF010000000
VERSION     DAZKGF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.058"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            15..1484
                     /gene="gspA"
                     /locus_tag="ACHOQV_002256"
     CDS             15..1484
                     /gene="gspA"
                     /locus_tag="ACHOQV_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFP9630624.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSSQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLMEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTSVFT
                     AKHWRMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTDPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHQWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1486..1905
                     /gene="gspB"
                     /locus_tag="ACHOQV_002257"
     CDS             1486..1905
                     /gene="gspB"
                     /locus_tag="ACHOQV_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFP9630625.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2143..2454
                     /gene="rpsJ"
                     /locus_tag="ACHOQV_002258"
     CDS             2143..2454
                     /gene="rpsJ"
                     /locus_tag="ACHOQV_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP9630626.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2487..3116
                     /gene="rplC"
                     /locus_tag="ACHOQV_002259"
     CDS             2487..3116
                     /gene="rplC"
                     /locus_tag="ACHOQV_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP9630627.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3127..3732
                     /gene="rplD"
                     /locus_tag="ACHOQV_002260"
     CDS             3127..3732
                     /gene="rplD"
                     /locus_tag="ACHOQV_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP9630628.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3729..4031
                     /gene="rplW"
                     /locus_tag="ACHOQV_002261"
     CDS             3729..4031
                     /gene="rplW"
                     /locus_tag="ACHOQV_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP9630629.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4049..4870
                     /gene="rplB"
                     /locus_tag="ACHOQV_002262"
     CDS             4049..4870
                     /gene="rplB"
                     /locus_tag="ACHOQV_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP9630630.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4887..5165
                     /gene="rpsS"
                     /locus_tag="ACHOQV_002263"
     CDS             4887..5165
                     /gene="rpsS"
                     /locus_tag="ACHOQV_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP9630631.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5180..5512
                     /gene="rplV"
                     /locus_tag="ACHOQV_002264"
     CDS             5180..5512
                     /gene="rplV"
                     /locus_tag="ACHOQV_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP9630632.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5530..6231
                     /gene="rpsC"
                     /locus_tag="ACHOQV_002265"
     CDS             5530..6231
                     /gene="rpsC"
                     /locus_tag="ACHOQV_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP9630633.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6244..6654
                     /gene="rplP"
                     /locus_tag="ACHOQV_002266"
     CDS             6244..6654
                     /gene="rplP"
                     /locus_tag="ACHOQV_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP9630634.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6654..6845
                     /gene="rpmC"
                     /locus_tag="ACHOQV_002267"
     CDS             6654..6845
                     /gene="rpmC"
                     /locus_tag="ACHOQV_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP9630635.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6845..7099
                     /gene="rpsQ"
                     /locus_tag="ACHOQV_002268"
     CDS             6845..7099
                     /gene="rpsQ"
                     /locus_tag="ACHOQV_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP9630636.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7264..7635
                     /gene="rplN"
                     /locus_tag="ACHOQV_002269"
     CDS             7264..7635
                     /gene="rplN"
                     /locus_tag="ACHOQV_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP9630637.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7646..7960
                     /gene="rplX"
                     /locus_tag="ACHOQV_002270"
     CDS             7646..7960
                     /gene="rplX"
                     /locus_tag="ACHOQV_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP9630638.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7975..8514
                     /gene="rplE"
                     /locus_tag="ACHOQV_002271"
     CDS             7975..8514
                     /gene="rplE"
                     /locus_tag="ACHOQV_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP9630639.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8529..8834
                     /gene="rpsN"
                     /locus_tag="ACHOQV_002272"
     CDS             8529..8834
                     /gene="rpsN"
                     /locus_tag="ACHOQV_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9630640.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8868..9260
                     /gene="rpsH"
                     /locus_tag="ACHOQV_002273"
     CDS             8868..9260
                     /gene="rpsH"
                     /locus_tag="ACHOQV_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP9630641.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9273..9806
                     /gene="rplF"
                     /locus_tag="ACHOQV_002274"
     CDS             9273..9806
                     /gene="rplF"
                     /locus_tag="ACHOQV_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP9630642.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARAQLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9816..10169
                     /gene="rplR"
                     /locus_tag="ACHOQV_002275"
     CDS             9816..10169
                     /gene="rplR"
                     /locus_tag="ACHOQV_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP9630643.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10184..10687
                     /gene="rpsE"
                     /locus_tag="ACHOQV_002276"
     CDS             10184..10687
                     /gene="rpsE"
                     /locus_tag="ACHOQV_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP9630644.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10691..10870
                     /gene="rpmD"
                     /locus_tag="ACHOQV_002277"
     CDS             10691..10870
                     /gene="rpmD"
                     /locus_tag="ACHOQV_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP9630645.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10874..11308
                     /gene="rplO"
                     /locus_tag="ACHOQV_002278"
     CDS             10874..11308
                     /gene="rplO"
                     /locus_tag="ACHOQV_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP9630646.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11316..12647
                     /gene="secY"
                     /locus_tag="ACHOQV_002279"
     CDS             11316..12647
                     /gene="secY"
                     /locus_tag="ACHOQV_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP9630647.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12679..12795
                     /gene="rpmJ"
                     /locus_tag="ACHOQV_002280"
     CDS             12679..12795
                     /gene="rpmJ"
                     /locus_tag="ACHOQV_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP9630648.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12942..13298
                     /gene="rpsM"
                     /locus_tag="ACHOQV_002281"
     CDS             12942..13298
                     /gene="rpsM"
                     /locus_tag="ACHOQV_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP9630649.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13315..13704
                     /gene="rpsK"
                     /locus_tag="ACHOQV_002282"
     CDS             13315..13704
                     /gene="rpsK"
                     /locus_tag="ACHOQV_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP9630650.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13738..14358
                     /gene="rpsD"
                     /locus_tag="ACHOQV_002283"
     CDS             13738..14358
                     /gene="rpsD"
                     /locus_tag="ACHOQV_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP9630651.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14384..15373
                     /gene="rpoA"
                     /locus_tag="ACHOQV_002284"
     CDS             14384..15373
                     /gene="rpoA"
                     /locus_tag="ACHOQV_002284"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP9630652.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15414..15797
                     /gene="rplQ"
                     /locus_tag="ACHOQV_002285"
     CDS             15414..15797
                     /gene="rplQ"
                     /locus_tag="ACHOQV_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP9630653.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15904..16272
                     /gene="yhdN"
                     /locus_tag="ACHOQV_002286"
     CDS             15904..16272
                     /gene="yhdN"
                     /locus_tag="ACHOQV_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFP9630654.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILADDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16283..16708
                     /gene="zntR"
                     /locus_tag="ACHOQV_002287"
     CDS             16283..16708
                     /gene="zntR"
                     /locus_tag="ACHOQV_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFP9630655.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16764..16982
                     /gene="arfA"
                     /locus_tag="ACHOQV_002288"
     CDS             16764..16982
                     /gene="arfA"
                     /locus_tag="ACHOQV_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFP9630656.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVERNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16979..17389)
                     /gene="mscL"
                     /locus_tag="ACHOQV_002289"
     CDS             complement(16979..17389)
                     /gene="mscL"
                     /locus_tag="ACHOQV_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP9630657.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPTPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17519..18895)
                     /gene="trkA"
                     /locus_tag="ACHOQV_002290"
     CDS             complement(17519..18895)
                     /gene="trkA"
                     /locus_tag="ACHOQV_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFP9630658.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18917..20206)
                     /gene="rsmB"
                     /locus_tag="ACHOQV_002291"
     CDS             complement(18917..20206)
                     /gene="rsmB"
                     /locus_tag="ACHOQV_002291"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP9630659.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLNWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPPADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20252..21199)
                     /gene="fmt"
                     /locus_tag="ACHOQV_002292"
     CDS             complement(20252..21199)
                     /gene="fmt"
                     /locus_tag="ACHOQV_002292"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP9630660.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21214..21723)
                     /gene="def"
                     /locus_tag="ACHOQV_002293"
     CDS             complement(21214..21723)
                     /gene="def"
                     /locus_tag="ACHOQV_002293"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9630661.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21853..22976
                     /gene="dprA"
                     /locus_tag="ACHOQV_002294"
                     /pseudo
     CDS             21853..22976
                     /gene="dprA"
                     /locus_tag="ACHOQV_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
     gene            22948..23421
                     /gene="smg"
                     /locus_tag="ACHOQV_002295"
     CDS             22948..23421
                     /gene="smg"
                     /locus_tag="ACHOQV_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFP9630662.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23450..23992
                     /gene="yrdD"
                     /locus_tag="ACHOQV_002296"
     CDS             23450..23992
                     /gene="yrdD"
                     /locus_tag="ACHOQV_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFP9630663.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23997..24569
                     /gene="tsaC"
                     /locus_tag="ACHOQV_002297"
     CDS             23997..24569
                     /gene="tsaC"
                     /locus_tag="ACHOQV_002297"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFP9630664.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24574..25392
                     /gene="aroE"
                     /locus_tag="ACHOQV_002298"
     CDS             24574..25392
                     /gene="aroE"
                     /locus_tag="ACHOQV_002298"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP9630665.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25389..25646
                     /gene="yrdB"
                     /locus_tag="ACHOQV_002299"
     CDS             25389..25646
                     /gene="yrdB"
                     /locus_tag="ACHOQV_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFP9630666.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25622..26176)
                     /gene="yrdA"
                     /locus_tag="ACHOQV_002300"
     CDS             complement(25622..26176)
                     /gene="yrdA"
                     /locus_tag="ACHOQV_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP9630667.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSAKNYVKWKDEYLDQGNQTQP"
     gene            complement(26220..>26432)
                     /locus_tag="ACHOQV_002301"
     CDS             complement(26220..>26432)
                     /locus_tag="ACHOQV_002301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630668.1"
                     /translation="EFQDFFSLLLRPSCEVFHVVVSTEAHYRDPNFLHKYFFDLFFCL
                     LLFHPFCCIRTQNGAFLHAKRLAQGQ"
BASE COUNT         6725 a         6254 c         6911 g         6542 t
ORIGIN      
        1 aaacaggaag ttctatgtct acgagaagag aagttattct ttcctggttg cgtgagaaac
       61 gtcagacctg gcgtctgtgc tatttgttgg gtgaggctgg aagtggaaaa acctggctgg
      121 cgcagcaact acaaaaagat aaacatcgcc gtgtgattac tttaagcctc gttgtttcct
      181 cgcaaggtaa ggccgcatgg atcgttaccg acgataatgc ggctgaacag ggctgccgtg
      241 acagcgcctg gacgcgagat gagatggcgg gtcaattact gcatgcgctt catcgaactg
      301 atagtcgctg ccctcttatt attattgaaa atgctcacct gaatcaccgc cgcatacttg
      361 atgatctcca acgcgcgata tctcttattc ctgacggtca atttttgctg atcggcagac
      421 ccgatcgaaa agtcgaacgt gattttaaaa aacagggcat tgaacttgtc tcaacaggac
      481 gtctgatgga gcacgagctt aaggcaagca tccttgaagg tcagaatatt gaccagcccg
      541 atctcctttt aactgccaga gttctgaaac ggatagcttt attatgtcgg ggagatcgca
      601 gaaagctggc gcttgccggc gaaacaatta gcttattaca gcaagccgag cagaccagcg
      661 ttttcactgc aaaacattgg cgaatgattt accgcgtatt aggtgataac cgcccccgga
      721 aaatgcagct tgccgtggtg atgagtggaa ccattatcgc acttacctgt ggttggctac
      781 tgctgtcctc tttcacagat ccgttccccg ttcctgcgtg gctggtaccc gtaacgccag
      841 tagttaaaca agatatgact aaggatattg ctcatgtagt gatgcgcgat agtgaggcgc
      901 taagcgtgtt atacggcgta tgggggtacg aggtcccagc tgattcggcc tggtgcgatc
      961 aggcagtaag agcggggctg gcatgtaaaa gtgggaatgc ttctttacaa acattggtag
     1021 atcagaattt gccctggatc gcatcgctta aagtcgggga caaaaaattg cctgttgtgg
     1081 tcgttcgcgt cggtgatgct accgttgatg tcctcgtagg gcagcaaacc tggacgctga
     1141 cacatcaatg gtttgagtcg gtatggacgg gtgattatct tctgttatgg aaaatgtcgc
     1201 cggaagggga gagtaccatt acgcgtgata gcagcgagga agagattctc tggctggaaa
     1261 cgatgttaaa ccgcgcattg catatttcga cagaaccttc ggcagagtgg cgtcccttgt
     1321 tggtagaaaa aataaagcaa tttcaaaaaa gccaccactt gaaaactgac ggtgttgttg
     1381 gcttttcaac gttagtacat ctctggcagg tggcagggga gagtgcctac ttatatcggg
     1441 atgaagcaaa tatttccccg gaaacaacag tgaaggggaa atgacatgtt tgaattctat
     1501 atcgccgcgc gtgagcagaa agaaacaggt caccctggta tcttctcccg acaaaaacac
     1561 agcaccataa tatatgtcat ttgtttattg ctgatttgcc tgtggtttgc aggaatggtg
     1621 ttggttggtg ggtatgccag gcagctatgg gcgctttgga tagtaaaagc agaagtcact
     1681 gtagaggctg aaacgcccgc ttttaaacaa tcaacacaac actatttctt caaaaaacag
     1741 ccactccccg tcgtcgaatc cgtcgaggag gaagatgacc cagacgtcgc ggttgagaat
     1801 gctccctctt cttctgaaga cgaagaaaat actgtagaag aaagcgacga gaaagccggt
     1861 ttaagagagc gtgtcaaaaa tgcgctgaac gaacttgaga gataacccga aggctgttta
     1921 ctttactagc aatacgcttg cgttcggtgg ttaagtatgt ataatgcgcg ggcttgtcgt
     1981 agttgacagc aggttcaatc tgaacctcag ttagccgaat ttggctacct aacaatgctc
     2041 ccaatcgggg agctacgtaa gaacggttac actctcccat caatcgtaat gggtctgagg
     2101 agtaatcatt ttcgtttata aaataattgg agctctggtc tcatgcagaa ccaaagaatc
     2161 cgtatccgcc tgaaagcgtt tgatcatcgt ctgatcgatc aagcaaccgc ggaaatcgtc
     2221 gagactgcca agcgcactgg tgcgcaggtc cgtggtccga tcccgctgcc gacacgcaaa
     2281 gagcgcttca ctgttctgat ctccccgcac gtcaacaaag acgcgcgcga tcagtacgaa
     2341 atccgtactc acttgcgtct ggttgacatc gttgagccaa ccgagaaaac cgttgatgct
     2401 ctgatgcgtc tggatctggc tgccggtgta gacgtgcaga tcagcctggg ttaatcaggt
     2461 cattgagcga ttgagaggtt gaaacaatga ttggtttagt cggtaaaaaa gtgggtatga
     2521 cccgtatctt cacagaagac ggcgtttcta tcccagtaac cgtaatcgaa gttgaagcaa
     2581 accgcgttac tcaggttaaa gacctggcta acgatggcta ccgtgctatt caggtaacca
     2641 ccggtgctaa aaaagctaac cgtgtgacca agcctgaagc tggccacttc gctaaagctg
     2701 gcgtagaagc tggccgtggt ctgtgggaat tccgcctggc tgaaggcgaa gagttcactg
     2761 taggtcagag cattagcgtt gaactgtttg ctgacgttaa aaaagttgac gtaactggca
     2821 cctctaaagg taaaggtttc gcaggtaccg ttaagcgctg gaacttccgt acccaggacg
     2881 ctactcacgg taactccttg tctcaccgcg ttccgggttc tatcggtcag aaccagactc
     2941 cgggcaaagt gttcaaaggc aagaaaatgg caggtcagat gggtaacgaa cgtgtaaccg
     3001 ttcagagcct tgacgtagta cgcgttgacg ctgagcgcaa cctgctgctg gttaaaggtg
     3061 ctgtcccggg tgcaaccggt agcgacctga tcgttaaacc agctgtgaag gcgtaaggag
     3121 atagcaatgg aattagtatt gaaagacgcg cagagcgcgc tgactgtttc cgaaactacc
     3181 ttcggtcgtg atttcaacga agcgctggtt caccaggttg ttgttgctta tgcagctggt
     3241 gctcgtcagg gtactcgtgc tcagaagact cgtgctgaag taactggttc cggtaaaaaa
     3301 ccgtggcgcc agaaaggcac cggccgtgcg cgttctggtt ctatcaagag cccgatctgg
     3361 cgttctggtg gcgtgacctt tgctgctcgt ccgcaggacc acagtcaaaa agttaacaag
     3421 aagatgtacc gcggcgcgct gaaaagcatc ctgtccgaac tggtacgtca ggatcgtctg
     3481 atcgttgtcg agaagttctc tgtagaagcg ccgaaaacta agctgctggc acagaaactg
     3541 aaagacatgg ctctggaaga tgtgctgatc atcaccggtg agctggacga aaacctgttc
     3601 ctggctgcgc gcaacctgca caaggttgac gtacgcgatg caactggtat cgacccggtt
     3661 agcctgatcg ccttcgacaa agtcgtaatg actgctgatg ctgttaagca agttgaggag
     3721 atgctggcat gattcgtgaa gaacgtctgc tgaaggtgct gcgtgcaccg cacgtttctg
     3781 aaaaagcgtc tactgcgatg gaaaaatcta acaccatcgt actcaaagtt gctaaagacg
     3841 cgaccaaagc agaaatcaaa gctgctgtgc agaaactgtt tgaagtcgaa gtcgaagtcg
     3901 ttaacaccct ggtagttaaa gggaaagtta aacgtcacgg acagcgtatc ggtcgtcgta
     3961 gcgactggaa aaaagcttac gtcaccctga aagaaggcca gaatctggac ttcgttggcg
     4021 gcgctgagta agtcggagga gtaatacaat ggcagttgtt aaatgtaaac cgacatctcc
     4081 gggtcgtcgc cacgttgtta aagtggttaa ccctgagctg cacaagggca aaccttttgc
     4141 tccgttgctg gaaaaaaaca gcaaatccgg tggtcgtaac aacaatggcc gtatcaccac
     4201 tcgtcatatc ggtggtggcc acaagcaggc ttaccgtatt gttgacttca aacgcaacaa
     4261 agacggtatc ccggcagttg ttgaacgtct tgagtacgat ccgaaccgtt ccgcgaacat
     4321 cgcgctggtt ttgtacaaag acggtgaacg ccgttacatc ctggccccta aaggcctgaa
     4381 agctggcgac cagattcagt ctggcgttga tgctgcaatc aaaccaggta acaccctgcc
     4441 gatgcgcaac atcccggttg gttctactgt tcataacgta gaaatgaaac caggtaaagg
     4501 cggtcagctg gcacgttccg ctggtactta cgttcagatc gttgctcgtg atggtgctta
     4561 tgtcaccctg cgtctgcgtt ctggtgaaat gcgtaaagta gaagcagact gccgtgcaac
     4621 tctgggcgaa gttggcaatg ctgagcatat gctgcgcgtt ctgggtaaag caggtgctgc
     4681 acgctggcgt ggtgttcgtc cgaccgttcg cggtaccgcg atgaacccgg tagaccaccc
     4741 acatggtggt ggtgaaggtc gtaactttgg taagcacccg gtaactccgt ggggcgttca
     4801 gaccaaaggt aagaagaccc gcagcaacaa gcgtactgat aaattcatcg tacgtcgccg
     4861 tagcaaataa ttttagagga taagccatgc cacgttctct caagaaaggt ccttttattg
     4921 acctgcactt gctgaagaag gtagagaaag cggtggaaag cggagacaag aagcccctgc
     4981 gcacttggtc ccgtcgttca acgatctttc ctaacatgat cggtttgacc atcgctgtcc
     5041 ataatggtcg tcagcacgtt ccggtatttg taaccgacga aatggtcggt cacaaactgg
     5101 gtgaattcgc accgactcgt acttatcgcg gccacgctgc tgataaaaaa gcgaagaaga
     5161 aataaggtag gaggaagaga tggaaactat cgctaaacat cgccatgctc gttcttctgc
     5221 tcagaaggtt cgccttgttg ctgacctgat tcgcggtaag aaagtgtcgc aggctctgga
     5281 tattttgacc tacaccaaca agaaagcggc tgtactggtc aagaaagttc tggaatctgc
     5341 cattgctaac gctgaacaca acgatggcgc tgacattgac gatctgaaag ttacgaaaat
     5401 tttcgtagac gaaggcccga gcatgaagcg cattatgccg cgtgcaaaag gtcgtgcaga
     5461 tcgcatcctg aagcgcacca gccacatcac tgtggttgtg tccgatcgct gagactctgg
     5521 agactagcaa tgggtcagaa agtacatcct aatggtattc gcctgggtat tgtaaaacca
     5581 tggaactcta cttggtttgc gaacaccaaa gaattcgctg acaacctgga cagcgatttt
     5641 aaagtacgtc agtacctgac taaggaactg gctaaagcgt ccgtatctcg tatcgttatc
     5701 gagcgtccgg ctaagagcat ccgtgtaacc attcacactg ctcgcccggg tatcgttatc
     5761 ggtaaaaaag gtgaagacgt agaaaaactg cgtaaggtcg tagcggacat cgctggcgtt
     5821 cctgcacaga tcaacatcgc cgaagttcgt aagcctgaac tggacgcaaa actggttgct
     5881 gacagcatca cttctcagct ggaacgtcgc gttatgttcc gtcgtgctat gaagcgtgct
     5941 gtacagaacg caatgcgtct gggcgctaaa ggtattaaag ttgaagttag cggccgtctg
     6001 ggcggcgcag aaatcgcacg taccgaatgg taccgcgaag gtcgcgtacc gctgcacact
     6061 ctgcgtgctg acatcgacta caacacctct gaagcgcaca ccacttacgg tgtaatcggc
     6121 gttaaagtgt ggatcttcaa aggcgagatc ctgggtggta tggctgctgt tgaacaaccg
     6181 gaaaaaccgg ctgctcagcc taaaaagcag cagcgtaaag gccgtaaata aggagcgtcg
     6241 ctgatgttac aaccaaagcg tacaaaattc cgtaaaatgc acaaaggccg taaccgcggt
     6301 ctggcgcagg gtacggatgt tagcttcggc agcttcggtc tgaaagctgt tggccgtggt
     6361 cgtctgactg cccgtcagat cgaagcagca cgtcgtgcta tgacccgtgc agttaagcgt
     6421 caaggtaaga tctggatccg tgtgttcccg gacaaaccga tcactgaaaa gccgctggca
     6481 gtgcgtatgg gtaaaggtaa aggtaacgtg gagtattggg ttgccttgat tcagccgggt
     6541 aaagtcctgt atgaaatgga cggtgttccg gaagagctgg cccgtgaagc attcaagctg
     6601 gcagcagcga aactgccgat taaaaccacc tttgtaacta agacggtgat gtaatgaaag
     6661 caaaagagct gcgtgagaag agcgttgaag agctgaacac cgagctgctg aacctgctgc
     6721 gtgagcagtt caacctgcgt atgcaggctg caagtggcca gctgcaacag tctcacctgt
     6781 tgaagcaagt gcgtcgcgat gtcgcacgcg ttaagacttt actgaacgag aaggcgggtg
     6841 cgtaatgacc gataaaatcc gtactctgca aggtcgcgtt gttagcgaca aaatggagaa
     6901 atccattgtt gttgctatcg aacgttttgt gaaacacccg atctacggta aattcatcaa
     6961 gcgtacgacc aaactgcacg tacatgacga gaacaacgaa tgcggtatcg gtgacgtggt
     7021 tgaaatccgc gaatgccgtc cgctgtccaa gactaaatcc tggacgctgg ttcgcgttgt
     7081 agagaaagcg gttctgtaat acagtacact ctctcgatac gaataaacgg ctcagaaatg
     7141 agccgtttat tttttctacc catatccttg aagcggtgtt ataatgccgc gccctcgata
     7201 tggggatttt taacgacctg attttcgggt ctcagtagta gttgacatta gcggagcact
     7261 aaaatgatcc aagaacagac tatgctgaac gtcgccgaca actccggtgc acgtcgcgta
     7321 atgtgtatca aggttctggg tggctcgcac cgtcgctacg caggcgtagg cgacatcatc
     7381 aagatcacca tcaaagaagc aattccgcgt ggtaaggtca aaaaaggtga tgtgctgaag
     7441 gcggtagtgg tgcgcaccaa gaagggtgtt cgtcgcccgg acggttctgt cattcgcttc
     7501 gatggtaatg cttgtgttct tctgaacaac aacagcgagc agcctatcgg tacgcgtatt
     7561 tttgggccgg taactcgtga gcttcgtagt gagaagttca tgaaaattat ctctctggca
     7621 ccagaagtac tctaaggagc gaatcatggc agcgaaaatc cgtcgtgatg acgaagttat
     7681 cgtgttaacc ggtaaagata aaggtaaacg cggtaaagtt aagaatgtcc tgtcttccgg
     7741 caaggtcatt gttgaaggta tcaacctggt taagaaacat cagaagccgg ttccggccct
     7801 gaaccaaccg ggtggcatcg ttgaaaaaga agccgctatt caggtttcca acgtagcaat
     7861 cttcaatgcg gcaaccggca aggctgaccg tgtaggcttt agattcgaag acggtaaaaa
     7921 agtccgtttc ttcaagtcta acagcgaaac tatcaagtaa tttggagtag tacgatggcg
     7981 aaactgcatg attactacaa agacgaagta gttaaaaaac tcatgactga gtttaactac
     8041 aattctgtca tgcaagtccc tcgggtcgag aagatcaccc tgaacatggg tgttggtgaa
     8101 gcgatcgctg acaaaaaact gctggataac gcagcagcag acctggcagc aatctccggt
     8161 caaaaaccgc tgatcaccaa agcacgcaaa tctgttgcag gcttcaaaat ccgtcagggc
     8221 tatccgatcg gctgtaaagt aactctgcgt ggcgaacgca tgtgggagtt ctttgagcgc
     8281 ctgatcacta ttgctgtacc tcgtatccgt gacttccgtg gcctgtccgc taagtctttc
     8341 gacggtcgtg gtaactacag catgggtgtc cgtgagcaga tcatcttccc agaaatcgac
     8401 tacgataaag tcgaccgcgt tcgtggtttg gatattacca ttaccactac tgcgaaatct
     8461 gacgaagaag gccgcgctct gctggctgcc tttgacttcc cgttccgcaa gtaaggtagg
     8521 gttactaaat ggctaagcaa tcaatgaaag cacgcgaagt aaaacgcgta gctttagctg
     8581 ataaatactt cgcgaaacgc gctgaactga aagcgatcat ctctgatgtg aacgcttccg
     8641 acgaagatcg ttggaacgct gttctcaagc tgcagactct gccgcgtgat tccagcccgt
     8701 ctcgtcagcg taaccgctgc cgtcaaacag gtcgtccgca tggtttcctg cggaagttcg
     8761 ggttgagccg tattaaggtc cgtgaagccg ctatgcgcgg tgaaatcccg ggtctgaaaa
     8821 aggctagctg gtaattgtca ccaattgaat cacgggagta aagacagatg agcatgcaag
     8881 atccgatcgc ggatatgctg acccgtatcc gtaacggtca ggccgcgaac aaagctgcgg
     8941 tcaccatgcc ttcctccaag ctgaaagtgg caatcgccaa cgtgctgaag gaagaaggtt
     9001 ttattgaaga ttttaaagtt gaaggcgaca ccaagcctga actggaactt actctgaagt
     9061 atttccaggg caaagctgtt gtagaaagca ttcagcgtgt cagccgccca ggtctgcgca
     9121 tctataagcg taaagatgag ctgccgaaag ttatggcggg tctgggtatc gcagttgttt
     9181 ctacctctaa aggtgttatg actgatcgtg cagcgcgcca ggctggtctt ggtggcgaaa
     9241 ttatctgcta cgtagcctaa tcggaggaaa aaatgtctcg tgttgctaaa gcaccggtcg
     9301 ttgttcctgc cggcgttgac gtaaaaatca acggtcaggt tattacgatc aaaggtaaaa
     9361 acggcgagct gactcgtact ctcaacgatg ctgttgaagt taaacatgca gataataccc
     9421 tgaccttcgg tccgcgtgat ggttacgcag acggttgggc acaggctggt accgcgcgtg
     9481 cccagctgaa ctcaatggtt atcggtgtta ccgaaggctt cactaagaag ctgcagctgg
     9541 ttggtgtagg ttaccgtgca gcggttaaag gcaatgtgat taacctgtct ctgggtttct
     9601 ctcatcctgt tgaccatcag ctgcctgcgg gtatcactgc tgaatgtccg actcagactg
     9661 aaatcgtgct gaaaggcgct gataagcagg tgatcggcca ggttgcagcg gatctgcgcg
     9721 cctaccgtcg tcctgagcct tacaaaggca agggtgttcg ttacgccgac gaagtcgtgc
     9781 gtaccaaaga ggctaagaag aagtaaggta acactatgga taagaaatct gctcgtatcc
     9841 gtcgtgcgac ccgcgcacgc cgcaagctcc aggagctggg cgcaactcgc ctggtggtac
     9901 atcgtacccc gcgtcacatt tacgcacagg taattgcacc gaacggttct gaagttctgg
     9961 tagctgcttc tactgtagaa aaagctatcg ctgaacaact gaagtacacc ggtaacaaag
    10021 acgcggctgc agctgtgggt aaagctgtcg ctgaacgcgc tctggaaaaa ggcatcaaag
    10081 atgtatcctt tgaccgttcc gggttccaat atcatggtcg tgtccaggca ctggcagatg
    10141 ctgcccgtga agctggcctt cagttctaag gtagaggtgt aagatggctc acatcgaaaa
    10201 acaagctggc gaactgcagg aaaagctgat cgcggtaaac cgcgtatcta aaaccgttaa
    10261 aggtggtcgt attttctcct tcacagctct gactgtagtt ggcgatggta acggtcgcgt
    10321 tggttttggt tacggtaaag cgcgtgaagt tccagcagcg atccagaaag cgatggaaaa
    10381 agcccgtcgc aatatgatta acgtcgcgct gaataacggt actctgcaac accctgttaa
    10441 aggtgttcat acgggttctc gcgtattcat gcagccggct tccgaaggta ccggtatcat
    10501 cgccggtggt gcaatgcgcg ccgttctgga agtcgctggg gttcataacg ttctggctaa
    10561 agcgtatggt tccaccaacc cgattaacgt ggttcgtgca actatcgatg gcctggaaaa
    10621 tatgaattcc ccagaaatgg tcgctgccaa gcgtggtaaa tccgttgaag aaattctggg
    10681 gaaataaacc atggcaaaga ctattaaaat tactcaaacc cgcagtgcaa tcggtcgtct
    10741 gccgaaacac aaggcaacgc tgcttggcct gggtctgcgt cgtattggtc acaccgtaga
    10801 gcgcgaggat actcctgcta ttcgcggtat gatcaacgcg gtttccttca tggttaaagt
    10861 tgaggagtaa gagatgcgtt taaatactct gtctccggcc gaaggctcca aaaaggcggg
    10921 taaacgcctg ggtcgtggta tcggttctgg cctcggtaaa accggtggtc gtggtcacaa
    10981 aggtcagaaa tctcgttctg gcggtggcgt acgtcgcggt ttcgagggtg gtcagatgcc
    11041 tctgtaccgt cgtctgccga aattcggctt cacttctcgt aaagcagcga ttacagccga
    11101 agttcgtctg tctgacctgg ctaaagtaga aggcggtgtt gtagacctga acacgctgaa
    11161 agcggctaac attatcggta tccagatcga gttcgcgaaa gtgatcctgg ctggcgaagt
    11221 cactactccg gtaactgttc gtggcctgcg tgttactaaa ggcgctcgtg ctgctatcga
    11281 agctgctggc ggtaaaatcg aggaataagt agcagatggc taaacaaccg ggattagatt
    11341 ttcaaagtgc caaaggtggc ttaggcgagc tgaaacgcag actgctgttt gttatcggtg
    11401 cgctgattgt gttccgtatt ggctctttta ttccgatccc tggtattgat gccgctgtac
    11461 ttgccaaact gcttgagcaa cagcgaggca ccatcattga aatgtttaac atgttctctg
    11521 gtggtgctct cagccgtgct tctatctttg ctctggggat catgccatat atttcggcat
    11581 cgatcattat ccagcttctg acggtggttc acccaacgtt ggcagaaatt aagaaagaag
    11641 gggagtctgg tcgtcgtaag atcagccagt acacccgcta cggtactctg gtgctggcaa
    11701 tattccagtc gatcggtatt gctaccggtc tgccgaatat gcctggtatg caaggcctgg
    11761 tcattaaccc gggctttgca ttctacttca ccgctgttgt aagtctggtc acaggaacca
    11821 tgttcctgat gtggttgggc gaacagatta ctgaacgagg tatcggcaac ggtatttcaa
    11881 tcattatctt cgccggtatt gtcgcgggac tcccgccagc cattgcccat actatcgagc
    11941 aagcgcgtca aggcgacctg cacttcctcg tgttgctgtt ggttgcagta ttagtatttg
    12001 cagtgacgtt ctttgttgta tttgttgagc gtggtcaacg ccgcattgtg gtaaactacg
    12061 cgaaacgtca gcaaggtcgt cgtgtctatg ctgcacagag cacacattta ccgctgaaag
    12121 tgaatatggc gggggtaatc ccggcaatct tcgcttccag tattattctg ttcccggcga
    12181 ccatcgcgtc atggttcggg ggcggtactg gttggaactg gctgacaaca atttcgctgt
    12241 atttgcagcc tgggcaaccg ctttatgtgt tactctatgc gtctgcaatc atcttcttct
    12301 gtttcttcta cacggcgttg gttttcaacc cgcgtgaaac agcagataac ctgaagaagt
    12361 ccggtgcatt tgtaccagga attcgtccgg gagagcaaac ggcgaagtat atcgataaag
    12421 taatgacccg cctgaccctg gttggtgcgc tgtacattac ctttatctgc ctgatcccgg
    12481 agttcatgcg tgatgcaatg aaagtaccgt tctactttgg tgggacctca ctgcttatcg
    12541 ttgttgtcgt gattatggac tttatggctc aagtgcaaac tctgatgatg tccagtcagt
    12601 atgagtctgc attgaagaag gcgaacctga aaggctacgg ccgataattg gtcgcccgag
    12661 aagttacgga gagtaaaaat gaaagttcgt gcttccgtca agaaattatg ccgtaactgc
    12721 aaaatcgtta agcgtgatgg tgtcatccgt gtgatttgca gtgccgagcc gaagcataaa
    12781 cagcgccaag gctgattttt tcgcatattt ttcttgcaaa gttgggttga gctggctaga
    12841 ttagccagcc aatcttttgt atgtctgtgc gtttccattt gagtatcctg aaaacgggct
    12901 tttcagcatg gaacgtacat attaaatagt aggagtgcat agtggcccgt atagcaggca
    12961 ttaacattcc tgatcataag catgccgtaa tcgcattaac ttcgatttat ggcgtcggca
    13021 agacccgttc taaagccatc ctggctgcag cgggtatcgc tgaagatgtt aagatcagtg
    13081 agctgtctga aggacaaatc gacacgctgc gtgacgaagt tgccaaattt gtcgttgaag
    13141 gtgatctgcg ccgtgaaatc agcatgagca tcaagcgcct gatggatctt ggttgctatc
    13201 gcggtttgcg tcatcgtcgt ggtctcccgg ttcgcggtca gcgtaccaag accaacgcac
    13261 gtacccgtaa gggtccgcgc aaaccgatca agaaataatc ggggtgattg aataatggca
    13321 aaggcaccaa ttcgtgcacg taaacgtgta agaaaacaag tctctgacgg cgtggctcat
    13381 atccatgctt ctttcaacaa caccatcgtg actatcactg atcgtcaggg taacgcgttg
    13441 ggttgggcaa cagccggtgg ttccggtttc cgtggttctc gcaaatccac tccgtttgca
    13501 gctcaggttg cagcagagcg ttgcgctgac gccgtgaaag aatacggcat caagaatctg
    13561 gaagttatgg ttaaaggtcc gggtccaggc cgcgaatcta ctattcgtgc tctgaacgcc
    13621 gcaggtttcc gcatcactaa cattactgat gtgactccga tccctcataa cggttgtcgt
    13681 ccgccgaaaa aacgtcgcgt ataacgcttc gttttccagg tttgttggag aaagaaaatg
    13741 gcaagatatt tgggtcctaa gctcaagctg agccgtcgtg agggcaccga cttattcctt
    13801 aagtctggcg ttcgcgcgat cgataccaag tgtaaaattg aacaagctcc tggccagcac
    13861 ggtgcgcgta aaccgcgtct gtctgactat ggtgtgcagt tgcgtgaaaa gcaaaaagtt
    13921 cgccgtatct atggtgtgct ggagcgtcag ttccgtaact actacaaaga agcagcacgt
    13981 ctgaaaggca acaccggtga aaacctgttg gctctgctgg aaggtcgtct ggacaacgtt
    14041 gtataccgta tgggcttcgg tgccactcgt gcagaagcac gtcagctggt tagccataaa
    14101 gcaattatgg taaacggtcg tgttgttaac atcgcttctt atcaggttag tccgaatgac
    14161 gttgtaagca ttcgtgagaa agcgaagaag cagtctcgcg tgaaagccgc tctggagctg
    14221 gctgagcagc gtgaaaagcc aacctggctg gaagttgatg ctggcaagat ggaaggtacg
    14281 tttaagcgta agccggagcg ttctgatctg tctgcggaca ttaacgaaca cctgatcgtc
    14341 gagctttact ccaagtaaag cttagtacca aagagaggac acaatgcagg gttctgtgac
    14401 agagtttcta aaaccgcgcc tggttgatat cgagcaagtg agttcgacgc acgccaaggt
    14461 gacccttgag cctttagagc gtggctttgg ccatactctg ggtaacgcac tgcgccgtat
    14521 tctgctctca tcgatgccgg gttgcgcggt gaccgaggtt gagattgatg gtgtactaca
    14581 tgagtacagc accaaagaag gcgttcagga agatatcctg gaaatcctgc tcaacctgaa
    14641 agggctggcg gtgagagttc agggcaaaga tgaagttatt cttaccttga ataaatctgg
    14701 cattggccct gtgactgcag ccgatatcac ccacgacggt gatgtcgaaa tcgtcaagcc
    14761 gcagcacgtg atctgccacc tgaccgatga gaacgcgtct attagcatgc gtatcaaagt
    14821 tcagcgcggt cgtggttatg tgccggcttc tacccgaatt cattcggaag aagatgagcg
    14881 cccaatcggc cgtctgctgg tcgacgcatg ctacagccct gtggagcgta ttgcctacaa
    14941 tgttgaagca gcgcgtgtag aacagcgtac cgacctggac aagctggtca tcgaaatgga
    15001 aaccaacggc acaatcgatc ctgaagaggc gattcgtcgt gcggcaacca ttctggctga
    15061 acaactggaa gctttcgttg acttacgtga tgtacgtcag cctgaagtga aagaagagaa
    15121 accagagttc gatccgatcc tgctgcgccc tgttgacgat ctggaattga ctgtccgctc
    15181 tgctaactgc cttaaagcag aagctatcca ctatatcggt gatctggtac agcgtaccga
    15241 ggttgagctc cttaaaacgc ctaaccttgg taaaaaatct cttactgaga ttaaagacgt
    15301 gctggcttcc cgtggactgt ctctgggcat gcgcctggaa aactggccac cggcaagcat
    15361 cgctgacgag taaccggatc acaggttaag gttttactga gaaggataag gtcatgcgcc
    15421 atcgtaagag tggtcgtcaa ctgaaccgca acagcagcca tcgccaggct atgttccgca
    15481 atatggcagg ttcactggtt cgtcatgaaa tcatcaagac gactctgcct aaagcgaaag
    15541 agctgcgccg cgtagttgag ccgctgatta ctcttgccaa gactgatagc gttgctaatc
    15601 gtcgtctggc attcgcccgt actcgtgata acgagatcgt ggcaaaactg tttaacgaac
    15661 tgggcccgcg tttcgcgagc cgtgccggtg gttacactcg tattctgaag tgtggcttcc
    15721 gtgcaggcga caacgcgccg atggcttaca tcgagctggt tgatcgttca gagaaagcag
    15781 aagctgctgc agagtaatct gaagcaacgt aaaaaaaccc gccccggcgg gtttttttat
    15841 acccgtagca tccccactta tctacaatag ctgtactctt tttgttcatc ccctggagta
    15901 tatatgtggt tacttgacca gtgggcagag cgtcatatag cagaagcgca agcgaaaggt
    15961 gagtttgata acctggcagg tagcggcgaa ccattgatac tggctgatga ttctcatgtg
    16021 ccaccggaat tacgtgcggg gtatcgcttg ctgaagaatg ccggttgctt accgccagaa
    16081 cttgagcagc ggagggaagc aattcagctt ctggatatcc tgagaggtat ccgtcacgat
    16141 gatccgcaat atcaagaggt tagccgtcga ttgtcattac tggaattgaa gctgcgacaa
    16201 gctggactga gcaccgattt tttacgcggc gattatgctg acaagttgtt gaataaaatc
    16261 aacgataact agtggagtat gtatgtatcg cattggtgag ctggcaaaaa tggcggaagt
    16321 aacacccgac acgattcgtt attacgaaaa acagcagatg atggagcatg aagtgcgtac
    16381 tgaaggtggg tttcgcctgt ataccgaaag cgatctccag cgattgaaat ttatccgcca
    16441 tgccagacaa ctaggtttca gtctagagtc gatccgcgag ttgctgtcga tccgtatcga
    16501 tcctgaacac catacctgtc aggagtcaaa aggcattgtg caggaaagat tgcaggaagt
    16561 cgaagcacgg atagccgagt tgcagagtat gcagcgttcc ctccaacgcc ttaacgatgc
    16621 ctgttgtggg accgcccata gcagtgttta ttgctcgatt cttgaagctc ttgaacaagg
    16681 ggcgagtggc gttaagagtg gctgttgatt ttttacactg gcaggattac actcgcgccg
    16741 ttaaataacc aattggagtt tttatgagcc gatatcagca tactaaaggg cagataaagg
    16801 ataatgcgat agaagcatta ctacatgatc ccttattccg acagcgcgta gagagaaata
    16861 agaaagggaa aggcagttac atgcgaaaag gaaaacatgg caatcggggg aactgggagg
    16921 ccagtggcaa gaaagtaaat cactttttta ccactggcct tctgctttca ggcgcttgtt
    16981 aagagcggtt attctgctct ttcagcaaat cacgaatttc tgtcagtaat acttcttctt
    17041 tagttggtgt aggcgcggct gctggttctt cttttttccg attcagtttg ttgattagct
    17101 taatcgccat aaagatggca aaggctacaa tcagaaaatc aaagacgttt tgaatgaaga
    17161 caccgtaatg catcacaaca gcagggatat ccccctgcgc atcgcgtagc gtgacagcaa
    17221 actgtttgaa atcgatcccg ccaattaata accccagagg tggcataatg atatcggcaa
    17281 ccagtgaaga tacaatcttc ccgaatgccg caccgataat gacacccacc gccaaatcca
    17341 ccacgttccc gcgcatcgca aattcgcgaa attctttaat aatgctcatg ttattctccc
    17401 tatgaagccg acaatcataa gtctaacaaa tgttaagcta ttttcctgcc aggagtgaat
    17461 ttaattaatc cctgataatc aaagggaaat aataaggcgt cattagacgc cttattaatt
    17521 acaagaagaa aggacttggc tggaagagtc tttcgacgtc ggtaataaac tttttatctg
    17581 tgaggaacat aattacgtga tcgccttgct caatgcgcag attgtcattg gcaatcatca
    17641 cgtcgtttcc acgtaccacc gctccaataa tcgttcctgg cggtagcttg atttcatcaa
    17701 tgactctgcc gacaacgcgt gaggtgcttt catcaccgtg agcaacggct tcaatagctt
    17761 ctgctacgcc gcggcgcagt gaggaaacac caacaatatc agcttttcgc acatggctaa
    17821 gcaacgcaga aatagttgct tgttgcggtg aaatcgcaat atcgatcacg ctcccctgaa
    17881 ccagatccac ataagcgcga cgctggatca ataccatcac ctttttcgca cccatacgtt
    17941 tggcaagcat ggcggacatg atattggctt cgtcatcgtt ggtgacagca ataaacagat
    18001 caacttgatc gatatgttct tcggccagca gttcttgatc cgacgcatca ccaaaaaaga
    18061 cgatcgtatt ctgcaacttt tccgccagtt cggcagcgcg ctgctgatta cgttcgatga
    18121 gtttaacgct gtaatctttt tccagacgac gcgccagccc tgcaccgata ttaccgccgc
    18181 caaccagcat gatccgctta tacggttttt ccagtcgctg taattcactc atcaccgcgc
    18241 ggatatgctg tgaagcggca ataaagaaca cttcatcacc agcttcaaca atggtcgaac
    18301 cttgcggacg aatggggcga tcgtggcgga aaattgctgc cacacgagta tcgatatggg
    18361 gcatatgttc gcgcatggtc gaaagtgcat taccaatcag cgggccgcca taataggctt
    18421 taaccacagc caggctgact ttaccctcag cgaagttcac cacctgcaat gcgccgggat
    18481 actcaatcag tcggtaaata ttatcgataa ccaactgctc tggtgcgatc agatgatcaa
    18541 tcggtacagc atctgaatga aatagtttat cggcatcgcg cacgtagtct ggtgagcgga
    18601 tacgagcgat gcgattaggg gtgttgaaaa gtgagtaggc tacctggcag gcaaccatat
    18661 tggtttcatc tgaactagtt acagcaacca gcatatcggc gtcgtcggca cctgcctccc
    18721 gcaatacgcg tggatgagag ccatgcccct gcacgactcg caggtcaaat ttatcctgca
    18781 aggtccgcag acgctcaccg ttggtatcga caacagtaat atcgttgttc tcgccaacca
    18841 ggttttccgc cagtgtgccg ccaacctggc cggcacccag aatgataatt ttcatcagtc
    18901 gcgacccgtt atctcatcac tttttgatta gcttagcgta aaagaagccg tcgccctctt
    18961 cggcaccagg cagattttgt ttacccggtt gctctggtgt tcctgtttcg caaagttcgg
    19021 catcagcggt acgttgcaaa aaggctttaa tctgcaggct attctcttcc ggtaacaccg
    19081 aacaggtggc atagaccagg gttccaccgg attttaaatg cggccaaatg gcgtcgagaa
    19141 tttcagactg caattgcgcg agttccggga tatcgcgatc gcgacgtaac catttaatat
    19201 ccggatggcg acgaatcaca ccggttgctg aacaaggcgc atctaataaa atgcgatcaa
    19261 actgttgctc gccacaccat tgggaagggt agcggccatc accttgtttc acggttgcct
    19321 tcataccaag gcgttttaaa ttgtcgtaaa cgcgagagag gcgctgttcg tcaatatcaa
    19381 ccgcaacaac ctgcgcttct ggtgccacct caaggatatg cgttgtttta ccgccggggg
    19441 cggcacaaag atccaaaatg tgttcaccgt tttgtggcgc aagccaggtc atgcaacctt
    19501 gtgctgaggc atcctgaacg gtaacccatc cgtcttcaaa accaggtagc gcatgaacag
    19561 gtgcaggtgt ttccagacgt acagcatcag ggtaatccgc aggcgggaaa cctttcattc
    19621 ctgcttcatc cagcaatgca agccagctgt cgcgggaatg atgcgtacga ttaacgcgca
    19681 gccacattgg cggacgctgg ttattggctt cgacgatgga ttgccactgc tctggatacg
    19741 ctttttgcag acgcttcagc aaccaggaag gatgcagata acgtgcatca ctggcattaa
    19801 actcggctaa taactcttct tgctgacgct ggaactggcg taatacaccg tttatcaacc
    19861 ctttaagttg cgggcgctta attgcgatag cgccttcaac cgtttcagcc agcgcagcat
    19921 gaggtggtat gcgggtataa agcagttgat acaaaccaac cataatcagg taatgcacgg
    19981 tccgctgttt gccggtcatc ggacgggcca ttaacttatt aatcagccag tttaactgcg
    20041 aaagcgtacg cagtacgcca aagcacaact cttgaagaag tgctttgtct ttatcggaaa
    20101 ctttttgctg gagcggtggc agaatgttgc ttaatgattg cccttgctcg acgacttgtt
    20161 caacggcctg ggccgccatg ctacgtaaat tacgttgttt tttcataagt ataaaaatgc
    20221 ccggcaagac cgggcttaga agagtggact atcagaccag acggttgccc ggaacaaacc
    20281 attcccgacg agagttcagg aggtcttgcg cgctcatcgc tttcttaccc gcaggttgta
    20341 acgagagcag gttcaggatg ccatcaccag tcgcaacctg aatgccttgt ttgttggctt
    20401 caaggatcgt tcctggtgca gcgttggttg ccgtatcaat gaccgatgct ttccagactt
    20461 taaccggctg tccttcaatt tccagccagc tcattggcca tggattgaaa gcgcgaatgc
    20521 agcgttcaag ctgtgctgcc gaaagtgacc agtcaatacg cgcttcttct ttactcaact
    20581 tctcggcgta agtgacaaga gtttcgtcct gaacttctgg tttcgccgtg ccgtctgcca
    20641 attgtttcaa cgtggtgata agcccttgtg ggccaagctc tgccagcttg tcgtacagcg
    20701 taccactggt atcttctgca gtaatcgggc aggagagctt atagagcatg tctccggtgt
    20761 ctaaaccaac atccatttgc ataatggtca caccagtttc tgcatcaccc gcccatagtg
    20821 agcgttggat tggtgcagca ccgcgccagc gtggcagcag tgaaccatga acgttgatac
    20881 agccaagacg cggcatctcc agcactgctt taggcagaat taagccatag gcgacgacaa
    20941 ccataacatc agcctgtaga tcggcgacca gttgctggtt ttcttgtgga cgcagggaaa
    21001 ccggttgaaa aacgggcaga cctttttcct cagccagaac tttaaccggg ctgggcatca
    21061 gttttttacc gcgtcctgcc ggtcggtctg gctgggtgaa cacgccaacg acgttatgac
    21121 cagaagacaa cagcgcgtcg agatgacgcg ctgcaaagtc aggtgtaccc gcaaaaataa
    21181 tacgtagtga ttctgacacg ttagttctta tccttaagcc cgggctttca gacgatccag
    21241 tttttcaact ttctgacgaa tacgttgttg tttcagcggt gacagataat ccataaacag
    21301 tttgccgacc aggtgatcca tctcatgctg aatacagatg gctaacagac cctctgcttc
    21361 cagttcaaat ggtttaccgt cgcggtccag ggcgcgaatt ttaactttct ctgcgcgtgg
    21421 cactaaagca cgttgttcag ggatcgacag gcatccttct tcaatgcctg tttcgccgct
    21481 tttttctaaa agctccggat tgattaacac cagccgttcg tcacggtttt ccgaaacatc
    21541 aataacaatg atacgttgat ggatatcgac ctgggttgcc gccaggccaa taccttcttc
    21601 tgcgtacatc gtctcgaaca tatcatcgac gatacgctga atttctgcat tcacttcttc
    21661 taccggttta gcaactttgc gaagccgctc gtccggaata tgtaacactt gcaaaactga
    21721 cataaatctc cagagatgtg ttcaggagtt agaaagatta tttcttctat tctagacaaa
    21781 tccccctctg attgacagca tcactgacca atcgcaaaga ttgctaaggc tgcttatggc
    21841 agggagataa ggatggtcga tacagaaatt tggctgcgtt taatgagcat cagcagcttg
    21901 tacggcgatg atatggtccg tatagctcac tggctggcaa aacagtcgca tattgatgcg
    21961 gttgtattgc agcaaactgg gcttacattg cggcaggcac aacgctttct ttcatttccg
    22021 cgaaagagta tcgaaagctc actttcttgg ttggagcaac ccaaccatca tttaattcct
    22081 gcggacagcg aattttatcc tcctcaactt cttgcgacga cagattaccc cggcgcactg
    22141 tttgttgaag gagaactgca cgcgctgcat tcatttcagc ttgccgtagt ggggagtcgg
    22201 gcgcattcat ggtatggcga gcgatgggga cgattatttt gcgaaactct ggcgacgcgt
    22261 ggagtgacaa ttacgagtgg actgcgcgtg gaatcgatgg tgtggcgcat aaagcggcct
    22321 tacaggtaaa tggcgtcagc attgctgtat tggggaatgg acttaatacc attcatccgc
    22381 gtcgccatgc ccgactggct accagtttgc ttgaacatgg tggggctctt gtctcggaat
    22441 ttcccctcga tgttccaccc cttgcttaca atttcccacg aagaaatcgc attatcagtg
    22501 gtctaagtaa aggtgtactg gtggtggaag cggctttgcg cagtggttcg ctggtgacag
    22561 cacgttgtgc gcttgagcag gggcgtgaag tttttgcctt gccgggtcca ttagggaatc
    22621 cgggaagcga agggcctcac tggttaataa aacaaggtgc gattcttgtg acggaaccgg
    22681 aagaaattct ggaaaacttg caatttggat tgcactggtt gccagacgcc cctgaaaatt
    22741 cattttattc accagatcag gaagacgtgg cattgccatt tcctgagctc ctggctaacg
    22801 taggagatga ggtaacacct gttgacgtcg tcgctgaacg tgccggccaa cctgtgccag
    22861 aggtagtaac tcaactactc gaactggagt tagcaggatg gatcgcagct gtacccggcg
    22921 gctatgtccg attgaggagg gcatgccatg ttcgacgtac taatgtattt gtttgaaacc
    22981 tatattcaca cagaagctga gttgcgtgtg gatcaagaca aacttgaaca ggatcttacc
    23041 gacgcaggat ttgatcgaga agatatctac aatgccctgc tttggctgga aaagcttgcg
    23101 gattatcagg aaggactggc agaaccgatg caactggcct ctgatccact ctccatgcgt
    23161 atttataccc ctgaagagtg tgaaaggctg gatgccagct gtcgtggttt tctgcttttc
    23221 cttgagcaaa ttcaggtgct taaccttgaa acccgtgaaa tggtgataga gcgagtgctc
    23281 gcgctggata ccgcagagtt cgacctggaa gacctgaaat gggtaatcct gatggtgttg
    23341 ttcaatattc cgggctgcga aaatgcgtac cagcaaatgg aagaattact ctttgaagtg
    23401 aatgaaggta tgctgcatta atcatttctt taattcagca taagttgtta tggcgaaatc
    23461 agcactgttc acggtgcgta ataatgagtc ctgcccaaag tgcggggctg aactggttat
    23521 tcgatccggg aaacacggtc cgtttcttgg atgctcacag tatccggcgt gtgactacgt
    23581 ccgtcctctg aaatcttcag cggatggaca tattgtcaaa gttctggagg ggcaggtttg
    23641 ccctgcatgt ggcgcaaatc tggtattacg ccagggacgc tttggtatgt ttattggttg
    23701 cagtaactac cctgaatgcg aacataccga acttatcgat aaaccggacg aaacagcaat
    23761 tacctgtccc caatgtcgga cgggccatct ggtccagcgc cgttcccgtt atggcaaaac
    23821 atttcactcc tgtgatcgct acccggagtg tcaatttgcc attaacttca aacccatagc
    23881 tggagaatgc cctgagtgtc attatccgct actcatcgaa aagaaaaccg cgcagggtgt
    23941 aaaacacttt tgtgccagta aacaatgtgg aaagccggtt tcggcggaat aataacgtga
    24001 ataataacct gcaaggagac gctatcgcag ctgcgataga tgtcctcaat gaaaaacgtg
    24061 tcatcgccta tccaacggaa gccgttttcg gtgtcgggtg cgatcctgat agcgaaacag
    24121 cagtgatgcg actgttggag ttaaaacagc gtccggttga taaggggctg attttaatcg
    24181 cggcaaatta cgagcagctt aaaccctata ttgatgacac catgctgact gatgcgcagc
    24241 gtgaaaccat tttttcccgc tggccaggtc ctgtcacctt tgtctttccc gcgcctgcga
    24301 caacaccgcg ctggttgacg ggccgctttg attcgcttgc tgtacgagtc accgaccatc
    24361 cgttggtggt tgctttgtgc caggcttatg gtaaaccgct ggtttctacc agtgccaact
    24421 tgagtggatt gccaccttgt cgaacagtag acgaagttcg cgcacaattt ggcgcggcgt
    24481 tcccggttgt gcctggtgaa acgggggggc gtttaaatcc ttcagaaatc cgcgatgccc
    24541 tgacgggtga actgtttcga caggggtaac ataatggaaa cctatgctgt ttttggtaat
    24601 ccgatagccc acagcaaatc gccattcatt catcagcaat ttgctcagca actgaatatt
    24661 gaacatccct atgggcgcgt gttggcaccc atcaatgatt tcatcaacac actgaacgct
    24721 ttctttagtg ctggtggtaa aggtgcgaat gtgacggtac cttttaaaga agaggctttt
    24781 gccagagcgg atgagcttac tgaacgggca gcgttggctg gtgctgttaa taccctcaag
    24841 cggttagaag atggacgcct gctgggtgac aataccgatg gtataggttt gttaagcgat
    24901 ctggaacgtc tgtcttttat ccgtcctggt ttacgtattc tgctcatcgg cgctggtgga
    24961 gcttctcgcg gcgtactact gccacttctt tccctggact gtgcggtgac aatcactaat
    25021 cggacggtat cccgcgcgga agagttggct aaattgtttg cgcacactgg cagtattcag
    25081 gcgttgggta tggacgaact ggaaggtcat gagtttgatc tcattattaa tgcaacatcc
    25141 agtggcatca gtggtgatat tccggcgatc ccgtcatcgc ttattcatcc aggcatttat
    25201 tgctatgaca tgttctatca gaaaggaaaa actccttttc tggcatggtg tgagcagcga
    25261 ggctcaaagc gtaatgctga tggtttagga atgctggtgg cacaggcggc tcatgccttc
    25321 cttctttggc atggcgttct gcctgatgta gaaccggtca tcaaactatt gcaacaggaa
    25381 ttgtcagcgt gaatcaggcc atccagtttc cggataggga agagtgggac gagaataaaa
    25441 gatgtgtatg ttttcccgct ctcgtgaatg gtatgcaact gacatgtgcg atctctggcg
    25501 agagtctggc gtatcgcttt actggagata cgccagaaca gtggttagcg agttttcgtc
    25561 agcatcgctg ggacctggaa gaagaagcgg aaaacttaat tcaggaacaa agtgaagatg
    25621 atcaaggttg ggtctggtta ccctgatcca gatattcgtc cttccatttc acgtaattct
    25681 tcgcggaata gcgtaaccca gccttctctt catcacttaa cgggcggatc tgtttgacgg
    25741 ggctaccgag atacagatat ccgctctcca gccgtttatt ttgtgggacc aggctacccg
    25801 caccaatcat cacatcatct tctactattg cgccatcaag taaaattgag cccatcccaa
    25861 ccaaaactcg attgccaatg gtgcagccgt ggagcatcac cttgtgacca acagtgacat
    25921 cttcgccaat ggttaatggg ttgccatctg ggttgtacga ggatttatga gtgacatgca
    25981 acatactgcc atcctggata ttggtgcgtg ctccgatctg tacataatgt acatctccac
    26041 gaatcacaac gagcggccag atccccacat catcagccag acgaacgtca ccaatcacga
    26101 cgctgctatc gtcgatcatt acgcgctggc cgatttgtgg aaaaagatcg cggtatgggc
    26161 gtaaaacatc agacatactc acctcaacaa taaatgattt actaatgact ttgggtgcat
    26221 tattggcctt gtgcaagtct tttagcatgc aaaaaagcac cgttttgtgt gcgaatgcag
    26281 caaaaagggt gaaaaagcaa caaacagaaa aaaagatcaa aaaagtactt gtgcaaaaaa
    26341 ttgggatccc tataatgcgc ctccgttgag acgacaacgt gaaacacttc acaagatggt
    26401 cgtaacaaca aagagaaaaa atcctgaaat tc
//
LOCUS       DAZKGF010000059        26200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZKGF010000059 DAZKGF010000000
VERSION     DAZKGF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.059"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..499)
                     /gene="curA"
                     /locus_tag="ACHOQV_002302"
     CDS             complement(<1..499)
                     /gene="curA"
                     /locus_tag="ACHOQV_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFP9630669.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDIATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATV"
     gene            680..1195
                     /gene="mddA"
                     /locus_tag="ACHOQV_002303"
     CDS             680..1195
                     /gene="mddA"
                     /locus_tag="ACHOQV_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFP9630670.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERSEPDAI"
     gene            1195..1644
                     /gene="ydcZ"
                     /locus_tag="ACHOQV_002304"
     CDS             1195..1644
                     /gene="ydcZ"
                     /locus_tag="ACHOQV_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9630671.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSNVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRTF"
     gene            complement(1645..1878)
                     /gene="ydcY"
                     /locus_tag="ACHOQV_002305"
     CDS             complement(1645..1878)
                     /gene="ydcY"
                     /locus_tag="ACHOQV_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFP9630672.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREERYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1964..2137)
                     /gene="ortT"
                     /locus_tag="ACHOQV_002306"
     CDS             complement(1964..2137)
                     /gene="ortT"
                     /locus_tag="ACHOQV_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFP9630673.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2332..2427
                     /gene="yncL"
                     /locus_tag="ACHOQV_002307"
     CDS             2332..2427
                     /gene="yncL"
                     /locus_tag="ACHOQV_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFP9630674.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2524..3948)
                     /gene="patD"
                     /locus_tag="ACHOQV_002308"
     CDS             complement(2524..3948)
                     /gene="patD"
                     /locus_tag="ACHOQV_002308"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFP9630675.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(3970..4764)
                     /gene="ydcV"
                     /locus_tag="ACHOQV_002309"
     CDS             complement(3970..4764)
                     /gene="ydcV"
                     /locus_tag="ACHOQV_002309"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9630676.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAVYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(4754..5695)
                     /gene="ydcU"
                     /locus_tag="ACHOQV_002310"
     CDS             complement(4754..5695)
                     /gene="ydcU"
                     /locus_tag="ACHOQV_002310"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9630677.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWGNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLIMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(5696..6709)
                     /gene="ydcT"
                     /locus_tag="ACHOQV_002311"
     CDS             complement(5696..6709)
                     /gene="ydcT"
                     /locus_tag="ACHOQV_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9630678.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKSASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVDKKQRYAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDALMADKLCGMTGSFALRPEHIRFNIPGEVQANGT
                     IQAVQYQGAATRFELRLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(6727..7872)
                     /gene="ydcS"
                     /locus_tag="ACHOQV_002312"
     CDS             complement(6727..7872)
                     /gene="ydcS"
                     /locus_tag="ACHOQV_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFP9630679.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGIRDPYQLTEEQY
                     QAVLKVMRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMSWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(8117..9523)
                     /gene="ydcR"
                     /locus_tag="ACHOQV_002313"
     CDS             complement(8117..9523)
                     /gene="ydcR"
                     /locus_tag="ACHOQV_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9630680.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGHIIARPQSGYYVATQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLKALELALQEYPVKACWLMTNSQNPLGFTLTPQKKARLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(9602..10018)
                     /gene="hicB"
                     /locus_tag="ACHOQV_002314"
     CDS             complement(9602..10018)
                     /gene="hicB"
                     /locus_tag="ACHOQV_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFP9630681.1"
                     /translation="MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(10062..10238)
                     /gene="hicA"
                     /locus_tag="ACHOQV_002315"
     CDS             complement(10062..10238)
                     /gene="hicA"
                     /locus_tag="ACHOQV_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFP9630682.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIK
                     EPLRKAILKQLGLN"
     gene            10460..10690
                     /gene="yncJ"
                     /locus_tag="ACHOQV_002316"
     CDS             10460..10690
                     /gene="yncJ"
                     /locus_tag="ACHOQV_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HFP9630683.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGHR"
     gene            complement(10782..12743)
                     /gene="rlhA"
                     /locus_tag="ACHOQV_002317"
     CDS             complement(10782..12743)
                     /gene="rlhA"
                     /locus_tag="ACHOQV_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFP9630684.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(12816..13352)
                     /gene="sutR"
                     /locus_tag="ACHOQV_002318"
     CDS             complement(12816..13352)
                     /gene="sutR"
                     /locus_tag="ACHOQV_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFP9630685.1"
                     /translation="MENLTRFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQVMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGARFAADVTHVYR
                     NGGEQIVHFHSLIHYPRS"
     gene            13405..14616
                     /gene="ydcO"
                     /locus_tag="ACHOQV_002319"
     CDS             13405..14616
                     /gene="ydcO"
                     /locus_tag="ACHOQV_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFP9630686.1"
                     /translation="MLRYLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDDQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGVVIVIAQGDVVTTDVVFKPVLPTYISPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            complement(14651..15319)
                     /gene="ydcL"
                     /locus_tag="ACHOQV_002320"
     CDS             complement(14651..15319)
                     /gene="ydcL"
                     /locus_tag="ACHOQV_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFP9630687.1"
                     /translation="MRTTSIAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPVTYYPVPKPSTQVGQKVLDKILSYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(15658..16251)
                     /gene="tehB"
                     /locus_tag="ACHOQV_002321"
     CDS             complement(15658..16251)
                     /gene="tehB"
                     /locus_tag="ACHOQV_002321"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFP9630688.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLENLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(16248..17240)
                     /gene="tehA"
                     /locus_tag="ACHOQV_002322"
     CDS             complement(16248..17240)
                     /gene="tehA"
                     /locus_tag="ACHOQV_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFP9630689.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLIRTERAVLMKAEDKE"
     gene            17364..18344
                     /gene="ydcK"
                     /locus_tag="ACHOQV_002323"
     CDS             17364..18344
                     /gene="ydcK"
                     /locus_tag="ACHOQV_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFP9630690.1"
                     /translation="MRKYRLSEEQRAFSYQDDGTKKSVLLRQIIAMSDFNDVIAGTAG
                     GWIDCETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIEKHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(18336..18875)
                     /gene="rimL"
                     /locus_tag="ACHOQV_002324"
     CDS             complement(18336..18875)
                     /gene="rimL"
                     /locus_tag="ACHOQV_002324"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFP9630691.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNRTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNAQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(18938..19162)
                     /gene="ydcH"
                     /locus_tag="ACHOQV_002325"
     CDS             complement(18938..19162)
                     /gene="ydcH"
                     /locus_tag="ACHOQV_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFP9630692.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            complement(19302..20957)
                     /gene="opgD"
                     /locus_tag="ACHOQV_002326"
                     /gene_synonym="mdoD"
     CDS             complement(19302..20957)
                     /gene="opgD"
                     /locus_tag="ACHOQV_002326"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFP9630693.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTDFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(21182..22525)
                     /gene="ydcJ"
                     /locus_tag="ACHOQV_002327"
     CDS             complement(21182..22525)
                     /gene="ydcJ"
                     /locus_tag="ACHOQV_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9630694.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGETHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            22742..23665
                     /gene="ydcI"
                     /locus_tag="ACHOQV_002328"
     CDS             22742..23665
                     /gene="ydcI"
                     /locus_tag="ACHOQV_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9630695.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(23703..25343)
                     /gene="trg"
                     /locus_tag="ACHOQV_002329"
     CDS             complement(23703..25343)
                     /gene="trg"
                     /locus_tag="ACHOQV_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFP9630696.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILSKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            25742..25891
                     /gene="hokB"
                     /locus_tag="ACHOQV_002330"
     CDS             25742..25891
                     /gene="hokB"
                     /locus_tag="ACHOQV_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFP9630697.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(25869..25937)
                     /gene="yncP"
                     /locus_tag="ACHOQV_002331"
     CDS             complement(25869..25937)
                     /gene="yncP"
                     /locus_tag="ACHOQV_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFP9630698.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(25963..26136)
                     /locus_tag="ACHOQV_002332"
     CDS             complement(25963..26136)
                     /locus_tag="ACHOQV_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630699.1"
                     /translation="MKKLALILVIGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCIN"
BASE COUNT         6550 a         6790 c         6282 g         6578 t
ORIGIN      
        1 ccaccgtcgc ccccacagga cctgtcgccg cagcaacaac caatgtttcg ccctctttgg
       61 gctgaccgat atccagcagc cccatataag cggtaaagcc tggcatcccc agcacaccca
      121 gcgaccacga tggattttgc ggatgatcgc caagtttcac cagatcatca ccactggata
      181 tgtcatagtc ctgccatcca ctgtagccca gcacccagtc gccaggctgg taatcaggat
      241 gattcgactc cacaacacgg ctcaccgtac cgccgaccat tacgccacca atatcaaccg
      301 gcggtgaata agatggctca tcgctcatac gtccacgcat atacgggtcc agtgacaaat
      361 aaactgtgcg cagtaacacc tgtccttcac ccggtgtggc gatatcatct tcttcaagac
      421 ggaaattctc cggaaccggc gcgccatgcg gacgcgaggc cagaacccaa cgtcgattac
      481 gctgcttttg ttgccccata atgctctcct gtgcttttgg catgaatcca tcaatagtag
      541 caggctcgca ggaatggttt tgtttgcagc cgtcacatcc cctctgcaac acgcaataat
      601 ttcatcgagg aatccgttgc ttcgggctgt tttcgatagt attgataacg tcatcatcat
      661 tatttaatcg aggccaaaca tgtccatccg ttttgcccgc aaagccgact gtgctgccat
      721 tgcggaaatt tataaccacg ccgtgttgta tacggcggct atctggaatg accaaacggt
      781 ggatgcagat aaccgcattg cctggtttga agcgcggacc atagcaggtt atccagtgct
      841 ggtgagcgaa gaagatggcg tagtgacggg atatgcctcg tttggcgact ggcgtagttt
      901 cgatggtttt cgccataccg tggaacattc ggtttatgtc catcccgatc atcagggcaa
      961 aggtctgggg cgtaaattgt taagccgatt gattgatgaa gcgcgggatt gcgggaagca
     1021 tgtcatggtc gccgggatcg aatcgcaaaa tcaggcgtct ttgcatctgc accagtcgct
     1081 gggatttgtc gtcaccgcgc aaatgccgca ggtgggcact aaatttgggc gctggctgga
     1141 cctgacattt atgcagttgc aactcgacga acgcagtgaa ccggacgcaa tctgatgaat
     1201 cagtcgctca cccttgcttt tttgattgcc gccgggattg gtctggtagt ccagaacacg
     1261 ctgatggtgc gcatcaccca gacctcctct accattctca tcgccatgtt gctgaactct
     1321 ttggtaggaa ttgtgctctt tgtttctatt ttatggttta aacaaggtat ggcggggttt
     1381 ggcgaactgg tgtccaacgt gcgctggtgg acacttatac ctggcttgct gggatcgttt
     1441 tttgtcttcg ccagtatcag cggatatcag aatgtgggag cggcgacaac tattgcggta
     1501 ctggtcgcca gccagctgat tggcggtctg gtgctggata tcttccgcag ccatggcgta
     1561 ccactacggg cgttgatcgg cccaatttgt ggcgcaattt tacttgtggt tggtgcctgg
     1621 ctggtggcca ggcgcacatt ttaatcagag gatggtgcca cctttggtta actgttcgtg
     1681 gcgcgcttcc atatcttctt tatgtttgcg accgtgatgg gcgatcgctg tgcgaagacg
     1741 ctgctgctgg gtatagcgtt cttcccgact taactccgca tcatcgctca gggcgattaa
     1801 taattcgttc atatgggcaa tgacgctctc ttcaatcgcg gcatccacgc gcgcgatgac
     1861 ttcatccaga tgcgacatac tttctcctta gccacttgtg aaatttatcg attcatccaa
     1921 atgatataag tctgcgtggt gccgtcaagc cagtgagtaa gatttaaaac agtgaaaaca
     1981 acagcgccac cgggaaactc atcggccatg ttgcccccac cagaaaggcg cttaagaaac
     2041 ggatgcgttt cttatcgtga gaaagaaacc aggtgataag aaatgcgatt gctgccataa
     2101 ccgcataaaa aacaagcatg tgttgataga gagacatgta cgggcgtcca gccagatgaa
     2161 taaaacgcgc gcaaatatgc gtcatttttt gacgtacatc aagtttttta accggtttga
     2221 gaaaaaaaca tcaaacagca acttgccctt atccatcttc agaatgagta ttattgcgga
     2281 ttttcttaac ccgtactata cacatagggg caatgataaa aggtggcaaa aatgaatgtt
     2341 tccagtagaa ctgtagtact gataaatgtc tttgctgctg ttggtttgtt tactcttatc
     2401 tctatgagat ttggctggtt tatttgatgt agtgattttg cacgcagcac gtactgtcag
     2461 agaaatcagt gctctgacag tacgcaagat catctcatac tcttacgatc tgcaatatta
     2521 tccttaatgt ttaaccatga cgtggcggac gacggtgtaa tcctccagcc cataaagtga
     2581 catatccttg ccgtaaccag aaagtttctg cccaccgtgc ggcatttcac ttaccagcat
     2641 gaaatgggta ttgacccagg tacaaccata ttgcagccgt gcgctgacgc gatgcgccct
     2701 gcccacatct ttcgtccata ccgaagatgc aagtccgtac tggctgtcat tcgcccagtt
     2761 taccacctgt tcttcgttgt cgaagagtgt aacactcact actggaccaa atacctcttt
     2821 ttgcacgatg gcatcgtcct gtaatgcgcc agccagcagc gtcggcgcat agtaatagcc
     2881 attacccttg cgcttttcac cgccagtgat cactttgatg tgccctgtcg ctttcgcctc
     2941 ttctactgcc ttgctgacgc gttcgagatg cgccagcgag cttaaaggtc caagctccgt
     3001 agactcgtca tccggcgcac cagattttaa cgttgccacc gcagcaccca gtttttccac
     3061 cagcgtatcg taaatgcctt tttgcgcgta gatccgacaa gccgcagtac aatcctgtcc
     3121 ggcattgtaa tagccaaatg tacgtacacc ttcgaccact gcctcaatat ccgcatcatc
     3181 aaaaacaatc actggcgctt tgccaccaag ttccatatga gtacgcttta tggacggcgc
     3241 ggtatggctg atgatgtgtt ccccagtggc gatagagccc gtcagcgaca ccatccgcac
     3301 tttgggatga ccggtcagcg gatcgcccac cgttttgcct ctgccaaaca gtacattaat
     3361 cacgccagcc gggaagatat ctttcgccaa ctctgccaac ttcaacgctg tcagcggggt
     3421 aatttctgat ggtttaagca ctacgcagtt ccctgccgcc agcgccggag ccagtttcca
     3481 cgcagccatc atcagaggat aattccacgg tgcgatagaa gccacgaccc ccaacggatc
     3541 gcgacggatc atcgaagtat gaccttcaag atattcacct gccgccagac cattcagaca
     3601 gcgcgccgca ccagcgaaaa agcgaaaaac atcgacaatc gccgggattt catcattgaa
     3661 cgcactatgc agcggtttgc cacaattacg ggactccagt tcggcaaaaa cctgaccatt
     3721 ttcttcgata acatcagcca gtttcagcag acattccgca cgcactttcg gcgtggtttg
     3781 cccccattcg gcaaatgctg catctgccgc gcgaacagca gcatcgacct gctctgcgga
     3841 tgcctcggca atctccagta aaacgtcccc cgttgccgga ttatagacag gctgtttttc
     3901 cccttcgccg ctaaccagtt ctccgttaat cagtaactta tgttgcatag catattcctg
     3961 tatcagtggt tattgaccat tgtcgccttc gcgggttagc caccaggccc ccaggatcgg
     4021 caaggttgtt accaacataa ccagcagtgc caccacgtta gttaccggta catcacgcgg
     4081 tcgcccaagc tgattgagca accacaacgg taacgttcgt tcatgacctg ccgtaaaggt
     4141 cgtaacgatg atttcatcga acgacaaggc aaacgccagc attcctcctg ccagtaacgc
     4201 cgaactgaga ttcggcaaca ctacgtagcg gaaggtttgc cagccattag ccccaagatc
     4261 cattgacgcc tcaaccagac tccacgaggt gcggcgaaaa cgggcgatga cattgttaaa
     4321 gaccaccact acacaaaaag tcgcatgacc gaccacgatg gtgaaaaatc ccggctccag
     4381 attgatggtt ttaaaggcgg ttaataacgc cagaccagtg acaatgcccg gcagcgcaat
     4441 gggcagcagc agtaacagcg aaatggcgtt tttgccgaaa aagtctcgtc gccacagcgc
     4501 ggcagcggcc agcgtcccta acaccagcgc gattaatgtc gccagcgccg ccactttaag
     4561 tgacagtgtc acggcatcaa gaatatcact acgctgtgct gccacgctaa accagcgcag
     4621 cgtcaggcct tgcggtggaa aactaaacgc cgcatcttca gtgttaaacg cataaacggc
     4681 gataatcaga atgggaaaat gtaagaaaac cacgccgccc caggccgcca gtttgaggaa
     4741 aaacggtgcg cgttcagagt gcatcgaacg ctcccagacg tttcacgaac gctaggtaaa
     4801 gtgcgatgag tacaatcggc actagggtga atgccgccgc catcggcata ttgccaatcg
     4861 ccccctgctg ggaataaacc atattgccga taaaatatcc tggagggcca accagctgcg
     4921 ggacaataaa atcgcccagc gtgagtgaga aggtaaagat agagccagcg gcaatacccg
     4981 ggattgccag cggcagcacc acatagcgaa aggtttgtcg tggacgtgcg ccgagatcag
     5041 ctgacgcctg caacaatgac ggcggcaaac gctcaagcgc cgcctgcacc gggaggatca
     5101 tgaacggcaa ccagatatag agaaacacca gaaagcgccc cagcccggaa gttgacagcg
     5161 tattacctcc caccgccggt agcgttagga acgcagtcag cagtggttcc agccccagat
     5221 gttgtaaaaa ccactgcgcc acgccatctt ttgccagcaa taacgtccag gcataggctt
     5281 tcacaatgta gctcgcccac atcggcaaca ttaccgcgat ataaaaaaac gctttcattt
     5341 tcccgctggt atagcgcgcc atataccacg ccattggaaa agccagaatg gcgctggcga
     5401 tagtgactgc cacagccata atcagcgtgc ggagaatgat gtcgtaattc gccggattaa
     5461 acagcgcacg gatattcgcc agcgtcagtt ccggcgttac cgacatggtg aaatcgtcaa
     5521 aggtataaaa tccctgccat aacagtgtca gcagcgagcc gaaatagaca atgccaaacc
     5581 acattagcgg gccaagcagc agtaaaaaca gccccagccc cggattaccc cagaagaaac
     5641 cggacacctt acccagacct ggacgtgaag gtgattgcaa tacgttcatc gccattcacc
     5701 tctcctcaac cagcggtacc atcacatcac gcgaccagga aaccatcacc tgttgtccgg
     5761 gcgtgagcgt ggcgggcagt tcttcgcctg tcatattggc ctgactcacc agcagttttt
     5821 caccgccgtt cagtctcagt tcaaaacggg tcgccgcgcc ctgatactgc accgcctgga
     5881 tcgtgccatt agcctgcact tcgccgggga tgttgaagcg gatatgttcc ggtcgcaggg
     5941 cgaagcttcc cgtcatgcca caaagtttgt ctgccatcag tgcatcaaaa acattcgatg
     6001 tcccgacgaa cccggcaaca aacggcgtgc gcgggcgcat atagagatca cgaggggaat
     6061 cgacctgctc aatgcgtcca ttattgaaaa ccgccacacg atcggacatc gataacgctt
     6121 cgccctgatc gtgggtaacg aagataaaag tgataccgag agactgttgc agttttttca
     6181 gttccagctg catctgctca cgcaatttga gatccagtgc gccgagcggt tcatccaaca
     6241 gcaatacgcg cggttcattc accaatgctc tggcgatagc aacccgctgg cgctgaccac
     6301 cagaaagctg tgacggctta cgttgctgaa caaaaccaag cgccactttc tccagcgctt
     6361 cttgcgccat agcatatcgc tgttttttat ccacgccttt gaccatcagc ccataggcca
     6421 cgttgtcaag aatcgacata tgcggaaaaa gtgcgtagtc ctggaagacg gtattaacat
     6481 cccgctccca cggtggcaga ttgctggcgg atttaccaaa gatagagata gccccgccgg
     6541 aaagctgttc gaagccagca atcaggcgca ggcaggtggt tttgccggag ccggacggcc
     6601 ccagcataga gaagaactca ccatctttta tcgcaatact gacgccatct actgcgcgca
     6661 cgtcaccgta caaccgtgag acgttgtcaa actccactgc gtacgtcata aaacacccca
     6721 gcgaagttag cgaccgccca taatggcaat gtaatcctgc gtccagcgac tgtagggaac
     6781 aaacttgccc ccttctgcta taggcgtttt ccagaaggcg attttatcga aatagttaaa
     6841 accgttggtt tcacaacctt tttcacctaa taacggactg gctttacacc cttgcggcac
     6901 tactggtaac gagccaaacc aggccgccac atcaccctgc acttttggcg ttaatgacca
     6961 gctcatccat ttgtaggcgc aaaccggatg tttcgcttcg ctatgtagca tggtggtgtc
     7021 agcccaaccg gtaacgccct ccttcgggaa aacggtagcg acaggctggc cttcggcttt
     7081 cagggcgttg gcctgatagg gccatgcact ggaagcaacc acaccctcgt ttttgaaatc
     7141 gctcatttgc acggtagtgt catgccagta gcgatggatc aaactatgtt gatcgcgcat
     7201 cactttcagt accgcctgat actgttcttc ggtgagttga tacggatcgc ggatgcccaa
     7261 ctgcggctga gtggctttaa cgaacaacgc agcgtccgca atgtagatag ggccatcata
     7321 agcctgaacg cggcctttat tgctcttgcc gtccggcaga ttctgctcaa caaaaaccac
     7381 ttgccagcta tccggcggcg tcgggaaggt tttagtgttg tacatcagca ggttcggccc
     7441 ccattggtaa ggtgtgccgt aaactttgcc gccaacgtta aaccagtcgc ctttaaccac
     7501 gcgcggatcg agcgctttcc agttgggaat caaggcggta ttaattggct gcacgcgttt
     7561 acccataatc aaacgcagcg aggcatcgcc ggatgccgta accagatcgt aacccccttt
     7621 ggccatcaga ctgaccattt catcggaagt cgcggcggtt ttaacattca ccgcgcagcc
     7681 tgtctctttt tcgaattgcg ttacccagtc gtattgttta tcagtttgtc cgcgttcgat
     7741 gtatcccggc caggcgataa tatccagtcg cccttccggt ttatctaaat tggtaggtgg
     7801 ttcggcggcg tgagcggtca ttattgtcat ggtgagcgca cacaggctgc tgcgggcaaa
     7861 tgtcttgctc ataaagtctt actcctgtcg taattaattg ttcagccgcc gtgccgtaaa
     7921 aatatctcct tgtttaacca tagacggcgg ctagcaggca caccttcgcc cctgagaaaa
     7981 tttaatcagg ttgtgagcta ccgcgcatta tgccgtatgt aaggttatct tctggagtat
     8041 agacaaggag ttaggaaata tgactgctat tcgaaattca tattacccaa cctgagagga
     8101 atttaattaa acgctattac agccgttctt gaataagttt gcctaattgt ttaaccgcct
     8161 gttcttcacg ttctccccac tgccacgcgg tattaaaacg gaaaaaacgt gaccagtttt
     8221 caccggtaga aaacatttta cccggcgcaa tactgatatg atgcgtcagt gctgccaggc
     8281 ttaattcgcc cgcatctaac ggctcgggga gctccagcca gagaaaataa ccgctgtcat
     8341 tatgatgaat tttcacttcc gcaggcagat aacgcagcag tgcttgccag gcacgttgtt
     8401 tacgttccgc aagctggcga cgcaggcgac gaagatgggc gtcgtatcgg cgcgtggaaa
     8461 ggtaatccac cagcgcaagc tgcatcggtg agctggtgga aagcgtactc atcaactgca
     8521 agcgttgaat tttacgtgca tgttttccgg cggcgaccca accaatacga aaaccaggca
     8581 ccagacattt cgaaaacgaa gagcaatgca aaacaccatc gtggcgatcc cacgctttcg
     8641 caggcagcgg tttttcccgt ccaaaataaa gttcgctgta aacgtcatct tcaatcagcg
     8701 ttacgttgta ctgattgagc aacgctacca gccgcgcttt tttctgcggc gttaaggtaa
     8761 atccgagtgg attctggcta ttagtcatca gccagcacgc tttcaccgga tactcctgca
     8821 acgccagttc cagcgccttg agatcaatcc cttctttaac gtccgtcgcc accgataacg
     8881 ccttcagccg taaccgctcc agcgcctgca acgcaccgta gaaacaagga ttctctacta
     8941 tcacccaatc gcccggttca gtgaccgctt gtaaactgag gtttaatgcc tctaacgccc
     9001 cggcagtaat gacaatttca tcgggtgaaa tggtgatgcc ctgtaaggca tagcgacgag
     9061 ctatagcctg acgcagttca gcgtttcctg gcggtaagtt ttcaatcacg ctcatcgccg
     9121 tggcggtttt gcttacctgt gccagcgagc ggttgagttg ttggaggggg aaaagtcgcg
     9181 ggtcgggaaa ggccgaggca aacggaacta ccgacggatc gcgactggcc tgcaacatat
     9241 caaaaatata ggtgttgata tcgactgctt catctcgagt gactggaatg actggcgctt
     9301 tcggcatttt tattgcctgt gtcgcaacgt aataacccga ctgcggtcgc gcgataatat
     9361 gtccctgact ttcgagcaac tgataggcat ggctgacagt cataaagctc atgccagaaa
     9421 gcgccacctg gtcacgcaac gaaggcaaac gatcgccagg ttgccagata cccgacgcaa
     9481 tctgctcgcg taattgttct gcaagctgct ggtatttttt cattatcgaa tcgtaattat
     9541 gtgcagatga ttcggcagtc tatatcagtt gttaaaaatg acaacttttc attaactgtt
     9601 atcaaaccat caccagtgat aactctttgc caagcgcctt agccgcaagc tggacggcgt
     9661 cgatttttgt agcatggtgc aagttaaata agcgagtaat ctcctgttta ggtttgccaa
     9721 ttcgtctggc taactcttgc tgagtgattt ctgactgtaa aaaagcattt aacagcaata
     9781 ctttagaggc gatacttaaa ggcacttcaa taaagtgatc gttgttagtt aatggcgaag
     9841 gcaacgggat tagctcatta tcttcaaaat aaaaatcaaa tgcggtgagt aaggcatctt
     9901 ttgctgcttc catcgcttca gcaacagttt ctccctgcgt caacgcttca gggatatcta
     9961 caaaagaaac cacataaccg ccttccggcg caggagtaag agtgacggga taacgcataa
    10021 ttttgactgt ctctcgcatg tcattaacct tcttatggcg attaatttaa accgagttgc
    10081 ttcaggattg ctttacgcaa tggttctttt atctcatcac tggggtgacg cggcatgaca
    10141 ctgcgtcgcc cttgaaacct gagtttcaaa tggttgctgc cattcgccac atcgacgccc
    10201 tgagattcga gccaacgtct gaactcgctt tgtttcacat cgcctccctg ccgctgagta
    10261 tatcaacaaa gtaaacatta gtgtttacct ccgtgtgtaa tttgctcgaa aatagcacta
    10321 aatcgccgca acggcagaat gcaatgcaaa atcaggaagg aagattccag aatttctttg
    10381 cgctaatttg caaaactgaa taataacctc aagaaacgat cggcatcata gcgacaaaag
    10441 gtaagaaaaa ggtggcagga tgtttacgaa ggcgttatcg gttgtcttat taacgtgtgc
    10501 actgttctca ggacaactca tggcagggca caaaggacat gaatttatgt gggtaaagaa
    10561 tgtggatcat cagctgcgtc atgaagcgga cagcgatgaa ttgcgtgctg tggcggaaga
    10621 gtcggcggaa ggtttgcgcg agcattttta ctggcaaaaa tcgcgcaaac cagaagcggg
    10681 acatcgttga gtcatttgtt gccggatgcg gcatgacgcc ttacccggca ggcaatatca
    10741 tgcagatttg atgagttgtg agtaaccaga aaactggcgt tttacccttt tcgcttcggt
    10801 aaagttttca gcagctcatc cggacttacg gatgccacta cgcttcccgg taacggcatt
    10861 ttcagtatgt gatttttgat tttgccaatg acgtgcatct cgcatgggcg gcaatcaaac
    10921 tttagcgtta atacttcatc gccgttaacc agttgcattg gcgtcgcctt ccagcttttg
    10981 atattgcctt tcgcctgttt cgggcacaga ttgaaggcaa agcgcagaca atgcttagtg
    11041 atcatcaccg ggacttcgcc cttctcttca tgcgcttcat acgccgcatc aatcagttgc
    11101 acaccatagc gatgataaaa ttcacgcgct ttctggttgt aaacgttcgc gaggaaactc
    11161 aaatgcgttt gcggataaac cggcgcagga tcagcaaccg gtttacggct gccgcgctgg
    11221 taactggcaa gacgcgcagc atctagcatg tcggcggctt cacggcggaa ctggtttaac
    11281 agactgtttg gtacaaacag cgcacccggc aaattaattt gcacatcgcg ggcgtaatag
    11341 atggtttgcc ccagttttgc cagaccatcc tttagattgt tcattgcttt ttcggcgtta
    11401 ttggcttcgt cgaactgccc gtccagcgta tgcgtgatgc tgacaccctc ttcactggtg
    11461 agggtcagaa tcagttgttc ctgccagccg cccagttcaa tgtctaccgc cacccgacgt
    11521 tcgctggagg tttttgtcag tgcctgctgc cagttatgat caagattacg gtttagtggg
    11581 tgatgcgggc gaattttgtg caactctgct ggcatttcat tgggccagac gcggtactga
    11641 ttttctccgg ttttctcgac cgtattggca cgaaagccga cgacttcacg tttaatcatc
    11701 acgttcaggc catcgccatt tgccagtggc tcggtaacgg caacatcgag atgatctttc
    11761 gccactttca atacttcacc caccggcagg ccgataaatt tcggcgaatc gaacgcgcca
    11821 atatcgcctt tacgggcatt cacaaaataa tctgtgctac cacggtggaa agtcttttcc
    11881 gtcgatggaa caaagaaatg ttcagtacga cctgatgaag cacgcgtcag atcgccacgt
    11941 tcttcaataa tggcatccag catctggcga taatgggcgg tgatattctt cacgtagctc
    12001 atatctttgt aacgcccttc aatcttgaag gagcgtacgc cagcatcaat cagcgcgccg
    12061 aggttggcag tctgatcgtt atctttcatc gacagcagat gtttttcata ggaaaccacc
    12121 cgcccctgat cgtctttcaa tgtgtatggc aaacggcacg cctgcgagca atcgccacgg
    12181 ttggcgctac gccctgtttg cgcatgagaa atgtagcact gacccgaata ggccacgcac
    12241 agtgcgccat gaataaagaa ttcaatggtc gcgtccgtgg cctggtgaat cgcgcggatc
    12301 tgctcaagat tcagctctcg cgccagcaca atctgcgtga agccaacatc agagaggaac
    12361 ttcgcttttt ctactgtacg aatgtcgcac tgcgtactgg cgtgcagttc aatcggcgga
    12421 atatcaagtt ccagaatccc catatcctga acaatcagcg cgtcgacgcc ggtctggtag
    12481 aggtcggtaa tcagccgttg cgcaggttcc agctcatcat catgcaaaat ggtgttaagc
    12541 gtgacgaaaa tttttgcacc ataacgatgg gcaaatggca ccagttcggc aatatctttc
    12601 aggctattac tggcattatg acgggcacca aaaccagggc cgccgatata aacagcatcg
    12661 gcaccgtgca aaatagcttc gcgggcaatg gcggcatcgc gtgccgggct taacagttca
    12721 agtcgatgag aagatacggt cacattctta tccagtagct gaaaaatggc ggctatttta
    12781 gccacaagcg cgacgttgcg aaatagtttt cccgcttaac tgcgtgggta atggatgagg
    12841 gaatgaaaat gtacgatttg ctccccacca ttacgataga catgcgtaac gtctgcagca
    12901 aatcgagcgc cttctccaca attaaggctc tgccattcac catcaacgca cagatcaagt
    12961 tgcccatcaa tgacgacaac atgttcgatt actcccttct catggggcgt cgattcactt
    13021 attgcaccag gtgccatctg aatcgagaag tgttcaaagc agagttgtgg atcataagga
    13081 aacagcgaag tgatcaccat cacctgctgt tgcggatcat aaactgaagg cgtagcggac
    13141 tgcggcggag aaataaatgt cgaaaatggc acattcaacc cggtggcaat tttccataat
    13201 gtcgcgaccg tcgggctgga ctcattacgc tcaatctgcc ctaacatcgc tttagaaacg
    13261 cccgtcgctt ctgccagtcg cgaaagactc cagccgcgct gctgacgtag ttgtttaagt
    13321 gtggtggata aaaagcgagt gagattttcc atgcgactcc tccggcaaaa cggaagttta
    13381 tcacttgtgc gttataacgg acaaatgcta cggtacctgt atgctataac gcacgaggtg
    13441 accatgcgtc tgttttctat tcctccaccc acgctactgg cggggtttct ggcggtatta
    13501 attggctacg ccagttcagc ggcaataatc tggcaagcag cgattgtcgc cggagccacc
    13561 actgcacaaa tctctggctg gatgacggcg ctggggctgg caatgggcgt cagtacgctg
    13621 gctctgacat tatggtatcg cgtacctgtt ctcaccgcat ggtcaacgcc tggcgcggct
    13681 ttgttggtca ccggattaca gggactaaca cttaacgaag ccatcggcgt ttttattgtc
    13741 accaacgcgt taatagtcct ctgcggcata acgggactct ttgctcgtct gatgcgcatt
    13801 attccgcact cgcttgcggc ggcaatgctt gccgggattt tattacgctt tggtttacag
    13861 gcgtttgcca gtctggatga tcaatttacg ttgtgtggca gtatgttgct ggtatggctg
    13921 gcaaccaagg ccgttgcacc gcgctatgcg gtaattgccg cgatgattat tggggtcgtg
    13981 atcgttatcg cgcaaggtga cgttgtcaca actgatgttg tctttaaacc cgttctcccc
    14041 acttatatta gccctgattt ttcgtttgct cacagcctga gcgttgcact cccccttttt
    14101 ctggtgacga tggcatcgca aaacgcaccg ggtatcgcag caatgaaagc agctggatat
    14161 tcggctcctg tttcgccatt aattgtgttt actggattgc tggcactgtt tttttcccct
    14221 ttcggcgttt attccgtcgg tattgcggca atcaccgcgg ctatttgcca aagcccggaa
    14281 gcgcatccgg ataaagatca acgttggctg gccgctgccg ttgccggcat tttttatttg
    14341 atcgcaggtc tgtttggtag tgccattacc gggatgatgg ctgccctgcc cgtaagttgg
    14401 atccagatgc tggcaggtct tgcgctgttg agtaccattg gcggcagttt gtatcaggcg
    14461 ctgcataatg agcgtgagcg cgacgcggcg gtggtggcat ttctggtaac ggcaagtgga
    14521 ttgacgctgg tcgggattgg ttctgcgttc tggggattaa ttgccggagg cgtttgttac
    14581 gtggtgttga atttaatcgc taacagaaac cgataataaa aagcgccatc taacgatggc
    14641 gcaactggta ttactttttg ttaacgtcaa acatggtggc atcggtcgcc atgtcatcaa
    14701 taacttgttt aatattttcg aaagccattg gcgtactttc gttattcagg tctttacctt
    14761 cgccctgacg aacgactttg ataaccggtt tattagtcgc tgcatcaatc agctcacctt
    14821 caaaatacag gcgagtatcc atggtacggt ggcccgtcgc catttgcgtc cccgcaacca
    14881 ctaatgcaac aggaaccact tcatagaatt gcagcccttc tttgctggta tctacaccgg
    14941 taatggcccc acggaaaatc agactacgcg gcccagcggt ggtaaccagt ggtttacgct
    15001 gcgcaatcgc ttctttcatt tcggtgttgg tataactcaa aattttatcc agaactttct
    15061 gccctacctg ggtagatggt ttcggtaccg gataataagt gactgggttc cagacgatgc
    15121 tgtcatattt gctttgatca aaactcgggt ctacccaacg taaaacaggt ttacctgttg
    15181 ctgaggttgt ttcttttaaa tcagagtaat tgtttaaaaa accagaatat ttatctggct
    15241 gggtgatttt agaggcacaa ccagacagag ccaacaagcc gcataaagct gcaactttcg
    15301 caattgatgt ggtacgcatg aatagtttcc tgagatgatt atattacttt ctaagttata
    15361 gcaaaaagcg ccgcttcgtg ttgtggcaaa aaaagtgttg gtgaaaataa attttttagc
    15421 cccttttcag acgacatagt caatgccgaa aaagacatag tcaatgccga aaaaggggct
    15481 gccgaaaaag gggctaagtc aatcatcagt agttaaattt cagtaagtta agctgctaaa
    15541 aaatttcgac tactgtgatt aatttcacat ccattcgata aaaaatatgc ttaccgaaaa
    15601 gaagaacagt aaaccatctc agcgtgggga attttcacgc gccttgttcc tagcgggtca
    15661 ttttttacgt gccagcatcg tggcgaaacg cagtttaata cgattaccgt tggcgtcggt
    15721 gcggtgcagc tcgccgacgt cttcattgta tttcaccatc tcccagcctt cgtaatatcg
    15781 acgtaattcc ccctctttga aggcaaacgg gaagccgacg gtacatggat aatcagcggt
    15841 atccatcgcc gccacaatca ggttgtaacc gccaggttta gtgcaacgtt gcatattggc
    15901 aatcagccct gggatggttt tagcctcaag gaacatcagc accacagtcg aaagaataaa
    15961 atcatactgt ccatcaaatg tgaggttatt cagatcaacg acccgggtgt gtaaattctc
    16021 cagattttca atggatttaa tccgctcgac gttggcgata ctcatggcat ttttatccca
    16081 tgcgtcaaca tcataaccat tggctgccag gtaaagactg ttacgaccat tgccacagcc
    16141 cagatccagc gttttacccg gtttaaccac tttcaccgct tccagcactt cagagtgtgt
    16201 gcgagttaat tcatatttat cagtaaaata gttttcgtca cgaatgatca ttctttgtcc
    16261 tctgctttca ttaaaacggc gcgctcggtt ctgattaaca attttccctg catcagaagc
    16321 gcaaaagtac ggatgagcag tattgcaata ataaaattgg taaagataaa cagcggcacc
    16381 gccagcgtat ggaaaaatcc attatcgctg ccactcccca gatgcaaacc ggtggttgcc
    16441 agtgcagata cgccgaacga gaaactccag aatgaagcat taaatggctg ggagagatac
    16501 catggcatca gacgtagcat aaacagcagt tgcagcagtc cataaccgaa aagcattttc
    16561 gccagcgtgt caccctcgcc gccgttgacg ctcagccagg cactacaggc caccagcgca
    16621 ggagcgagct gaatgccgag tgatgtccgc agcgccgtgg gtaattctcc cgaactgcgc
    16681 agacgctgca agatcacagg ttcaaggctt agccatgaga aaacgcctgc gcctaaaaac
    16741 accagaccgg cgtcggtgta gcccaacgca ccacaggcca ttgcgctgat aaagttgttg
    16801 gcaactgtcg gcagatacag tccaggcgtg gtagcttctt cagggtgaga tccgcgccat
    16861 aatcccgcag tttgccaggc ggcataagcc aactgaacca cgacgccaaa actgaacagg
    16921 cacaccgcca gtgggcgaaa ccacggaaca aaaccaatcg ccaccagcat cgtcgttgcc
    16981 ggaaacaaac tcacaaaact gctcagcact ggatggcgca cttccgccag cacgctatgc
    17041 ggaaagcgta tgagtcgggt aataaatgcg ctagttaata atccccagat gatcatcgcc
    17101 agaatcacca gcccatcccc taaccagtgg ctgacctgcc aaacctggct ggcatagcgc
    17161 caggcaaatc ccatccctat cgtccccaac acaataccaa agtagcctgc cggcaaattg
    17221 agcactttat cgctctgcat cttcttactc attttgttta atttataaag tatatttgaa
    17281 atgcatttta tggaattttc tcgagcatag ccagagccgc agaatttgct acggttatag
    17341 taagtcacct gtgggaaaac gctatgcgca aatatcgtct aagtgaagaa cagcgagcct
    17401 ttagttatca ggatgatggc actaaaaaaa gtgtgttatt acggcagatt atcgccatga
    17461 gcgattttaa cgatgtgata gcaggaaccg ctggcggctg gatcgattgc gaaacggtgt
    17521 tagcacagga aggaaattgc tggatttacg accagaatgc tattgcgttc ggcggagcgg
    17581 tgatttccgg taatacgcgc atcaccggca ccagcgtact gtggggagag gtttacgcaa
    17641 cggataacgt ctggatcgat aatagtgaaa tcagtcaggg tgcatatata agtgacagtg
    17701 tcaccattcg tgactcatta gtttgcgggc aatgtagaat atttggtcac gccttaattg
    17761 atcaacattc tatgatcgtc gccgcacaag gcttaacgcc cgaccatcaa ctcctgctac
    17821 aaatttatga tcgcgcaagg gttagcgcct cgcgtattgt tcaccaggca caaatttatg
    17881 gcgatgccgt cgtccggtat gcttttattg aacatcgcgc cgaagttttt gattttgcca
    17941 gcgttgaagg taatgaagag aacaacgtct ggctatgcga ttgtgctaaa gtctacggtc
    18001 acgcgcaggt gaaagctggc atagaagaag atgccattcc gacgattcat tacagttcgc
    18061 aggtggcgga atatgccatt gtggaaggta attgtgtgtt aaaacatcat gtcttgattg
    18121 gcggaaatgc cgtggtacgt ggcggaccga ttctgctcga cgagcacgtt gtaatacaag
    18181 gtgagagccg tattacaggt gcggtgataa ttgaaaaaca tgtggagtta accgaccacg
    18241 ccgtcgttga agcctttgat ggcgacacgg ttcatgtccg tggtccaaaa gtcattaacg
    18301 gcgaggagcg cattacgcga acgccgctgg cagggttatt gtgaatcgat aatacgcgcg
    18361 tataagttca catcatcata ggcatcattc aggaactcag cctgtttcag acaaccttca
    18421 aggataaaac cattacgcaa agcgacctgg ttgctttgcg cattgtccac ccgacatttg
    18481 atcacgaagc gtctaagttc accagactga gcgtaatgat gaatcaatgc ctgcagcgcc
    18541 tgagaaatga tcccctgccc ctgatgagat tcgtccagcc agtagccgat ttcagcggtt
    18601 ttattcagtg gttcaatacg attaaacgag ataacgccga taagttcatc ttctttgaaa
    18661 atcatgaaca ttttggcata gccgcgttga tgcaacatca cattaccctg caccgttttt
    18721 cgcgtgtcct cttcactttg aacaaactgc ggccagttta gcgactgctg taaccaggtt
    18781 ctatttttac agattaactg ataaagaggt gtgacatgac tttctgcaac agcatgtaat
    18841 tcaagtgatt cacttacttt tatcgtttca gtcatcttat ctccagtcaa tccatgcagt
    18901 tgttaaaaaa gccgcctggc tggcggctta ggaaagttta cacctctttg acgctcacct
    18961 gctgcaggat tttgagcatc tcatctttta actgtagctt ttgttttttc atgcggacca
    19021 cttccgcatt gtaccctcga ccgtcggaac cttcctttct ggcaatttca tgatcaagtt
    19081 tattgtgttt atcgaacaag gacataaagc gaggattttc gtttttcaga cgggatatta
    19141 aatctcggta ttctggaaac atacgcactc cctgttagtg gctgacaagg tgtatctgaa
    19201 atacacacca tcagattact catttaactt agggaaaaat gcgagcggga tcgtgtttaa
    19261 ccagacggca cctcccgaag aagatgccga agaaaaaacg attaactcat cacgcggtca
    19321 tcaacatact gacgtttatc cggcgctggc gggaaatact gatacagcca tgtttcactg
    19381 atagcgtcgc cctgacaacg cagatacatc cgcatatcga ccggatcagt ggagtccgaa
    19441 gtcggatacc agtcaaactg aatacgataa ccatcaatgg gttcaatata gagaatttcg
    19501 atttgtttcg cttccccact ggaaagcgta atcaccggct caatgccttt tggcgcggca
    19561 gctttcagat caccaccaac gaaatcgacg gcaaaacgtc gcgcccattt ttcgggatag
    19621 tgttcacctg gcgcccaacc ttccgggaaa ccgcccatgc cggtacgcgt cgccataacg
    19681 cgcgctaatg ggcaatgaac aggcggttgc gcactccagt acagacgata ctggaatgca
    19741 aactcatcac ccgcttttac cgctttttct ggctgccaga agcagacaat gttatccagc
    19801 gtttcgcccg ttgttgggat ttccatcagg ccgatggtgc ccttacccca cttgttacgc
    19861 ggctccaccc acagacttgg gcgtttgtta taccagccca taatgtcctg ataatgggag
    19921 aaatcacgat ccagttgcaa taaaccaaac cctttcgggt tgttgtcggt gtaagcattg
    19981 aactgcaatt tttgcggatt attcagcgga cgacaaatcc actcgccatt gccccgccac
    20041 atggacaaac gatcagagtc atggatttgc ggatgaatgg tgtcgcacat ccgacgttca
    20101 ttagtaccgc agctgaacat gctggtcatc ggcgcaatgc ccagctgttt aatgtctttg
    20161 cgcgcataca ggtgattttc cacatccata atcacctgac ttttctcaca atggatagtg
    20221 aacttatagg caccagtaat gctggcgcta tcgagcaacg cataaacggt aaaggtggtt
    20281 gcccccggtt ttaccgtatc aaaccagaag tcagtaaagt cggggaactc ttctttactg
    20341 tcggtgtaag tgtcgatcgc caggccgcgg gccgacaaac cgtattgata tgtatcatca
    20401 accgcgcgga aataactcgc gccgaggaat gatacgacat cacggcgcgc cagttcgggg
    20461 gctttaaaca cgcgaaaacc ggcaaaaccg agatcacttt gcccttctaa ttgtttggta
    20521 tcaacacccg catcgttgta tttgaacaac tccgggcgaa agtgaatttc acgcgccaga
    20581 tgtgttgctg gatctacaga aaacatacga acgcggcgac ggaatcccat tcccatatgg
    20641 aagaactgag cgtccagttg acggttctca acgttatgcc agagcgattt ttcggcgtcg
    20701 tattgaatac tgttataagc ctgcggcgtc attgtcgcca gagtgtcagg taacggacgc
    20761 ggcgcaccac gccacgctgt ttgcgctaag tcgtgcgcca ttgactgtag aatggagaag
    20821 tcaaaacgtt gggtttgccc gtcggcaata tcagaatctg ccgcgaatgc cgcctgagaa
    20881 aaaagagaag caatgccgct ggtaccgcac acggcggcca tagccattga acctttaata
    20941 aatcgtctac gatccatacc tgaaagtgag tccttctggt gttgtgaatg gtcccgcgct
    21001 gatcgttttc agcggcaaga aagtgcttat ttatgaccgc tcactctaga caatatttat
    21061 taataatcca attgttgaac tgagagaata tgcataaatc ggcaattttt ttatgtcgat
    21121 gggatgagcc tgattcgttt gtaaaatcag gcaaattttt gcaaaggcgg atggtcagaa
    21181 tttaaagcaa accacagcga cgtttactgc gttcttccgc ttcctgataa agctggaact
    21241 catccaaaac cgggcagccc aacgcctgct caaatgcttc gcgactggca ttaccgtgac
    21301 tgcgtgcctg cgtttcatta cccagatttg actggaagat ccccgccgcg ctaacaggca
    21361 agaaatcttc ataggtaatg ggttgcgcca ctacccaacc acgttcaatt aagggctgtg
    21421 gatcgtctcc gggatgaatc gcctgacgat gcgtctcacc tgaaggcgtc agacggtacc
    21481 ggaaccatgc caacccttgc tgacgcatta aaaactcact gtcaggaaaa gtgcggaagg
    21541 tttcctgtaa atgcatttgg tgagtgagat tatcctgccc ggttccagcg ttccgcagta
    21601 gatcatcata cagttgtcgt cctttcggcg ttaatgccac gccacgctgt tctatttcac
    21661 caaagcgcgc ggtatgcgtg ccctgtttct gccctgcaaa caacaccgtc tcttccagtg
    21721 ctttaaagct agtctggcgt agtaaaatcg gtacctcgcg gcgcggcggc ccttcgatca
    21781 gaattttggg ttcaattccg cattcaggca tcatcgactg cacccggtca atatccagcg
    21841 tgcgtggcgt caggtggttg atatggcatc caggaaaaca gaccacatca gcaatcaacc
    21901 gatgttcgtt gtgcaaggcg cgataggttt cttcatctac cgttgctgac tggtgccaac
    21961 gaaacgtttc cagggcttcc tgcacaaact cctgtgcctg tgtttcgtta aaaccgcccc
    22021 gctgctcata ttcctctaac agttgtcgac aacgtggggt gaagatatcg cgctgacgca
    22081 gaatctccgc cgctttctgg cgcaaaattt cgttctcgat aagctcaagg cggagtaatg
    22141 aagtaaaaac gcggaaggga ttacgcgcca gagaagcatc atcaatgggc cgaaatgctg
    22201 tggaatgtac cggcaccccc gcctgcgaga gatcgtaata gctcaccggg tacatcccca
    22261 taatggcaaa catccgccga agagtagcaa gctcttgtgc agtcccaaca cgaatcgccc
    22321 catgacgttc aacattcagt cgcgccagct cgtctgcatt taccattttt tcgtgcagtt
    22381 gaggattgtt ttccagcaca gccagattca catcagctac cagttccagc agcgtaccgt
    22441 actgcggaac ttcttgctgg tacatggctg acattgcctg cgaaaactgt tcccgaatct
    22501 catccgccgt gatgctgttc gccataatgt ctgcctccag tggtcagtaa tctggagtgt
    22561 aggtaaccgc attcactctg gcgggaagaa tttgcaaact gtgatctcgc cgcgaaaaca
    22621 tcaatattat ccatttcgct gtaacataat tgctttaatt gttaataata ttttgcaatc
    22681 aagttatcat aatcaaacaa cttcacttgt cagcgacacc gcttcgtttt taacatcgct
    22741 tatggaaaaa aatagtctgt ttagtcagcg catccgtttg cgccaccttc atacattcgt
    22801 agctgtcgca caacaaggaa ctttggggcg cgcggctgaa acccttaatt tgagtcaacc
    22861 tgcgctctct aagacattga atgaactgga gcagctgacg ggcgctcgct tgtttgagcg
    22921 tggtcgtcag ggggcgcaac ttaccttacc cggcgaacaa tttttaacgc atgcagtcag
    22981 agtgcttgac gccatcaaca ctgctggaca gtcgcttcat cgtaaagaag gtcttaataa
    23041 tgatgtcgtc agggttggtg cactaccgac tgcggcactg gggatattac cttcggttat
    23101 aggtcagttt catcagcaac aaaaagagac gaccttgcaa gttgcgacaa tgagtaaccc
    23161 tatgattctt gcgggtttaa aaaccgggga aatcgatatc ggcattggtc ggatgtcaga
    23221 tcctgaactg atgaccggac ttaattacga actgctgttt cttgaatcgc tgaagctggt
    23281 tgtccgccct aatcatccgc tacttcagga gaacgtaacg ctaagccggg tgctggaatg
    23341 gccggtcgtt gtatcaccag aaggcactgc gccacgccag cattcagatg cattagtgca
    23401 gagccaggga tgtaaaattc cttcgggttg tatcgaaacg ctgtctgctt cgctatctcg
    23461 tcaacttacg gttgaatacg attacgtgtg gtttgtccct tctggcgctg taaaagacga
    23521 cctgcgtcat gccacgctgg tggccctgcc tgttccgggt catggtgcag gcgaaccgat
    23581 tggaatactg acccgcgtag atgcgacgtt ctcttctggt tgccagttga tgattaacgc
    23641 tattcgaaaa tcaatgccgt tctgaaaggt gaagggatct gtcgatccct cctttaacat
    23701 tttcacactg tagcgaaact aactggttca ccagctccgc gaggttctgc cgacacagaa
    23761 tgtttgttca gacggaatac gtccaccgcc tcagttaatc gtgccgcctg ttcttcaagg
    23821 gacactgccg ctgctgaggc ctcttctacc agagaagcat tctgttgcgt caccttatcc
    23881 atttcagata tcgcctggct aacctgcgtt atacccctac tttgctcatc cgaggcggcg
    23941 gcaatttcct gcatgatatg tgtgacactc gcgacggcat caacaatagt gctcatggtt
    24001 ttcccggccg tggccacctc atccgatccc aggtcaatta acctgactga ttcactgatc
    24061 aagccttcaa tctctttcgc cgcctgagcg ctgcggcttg cgagtgtccg tacttcgctg
    24121 gcgacaacgg caaatccacg cccttgctca cccgctcgcg cggcttcaac ggcagcattc
    24181 agtgccagaa tattcgtctg gaaagcaata ctgttgatga cggcggtgat ctcagaaatt
    24241 ttcttcgaac tcgtggagat agcgcccatc gtttttacta caccggaaac cgtctgcccg
    24301 ccatcgctgg ctttaataga agcctcttgc gccagtttgc tggcatgatg cgcgttatcc
    24361 gcattctgtt tcaccgtcgc agtgagttgc tccatgctgg cggcagtttg ttcgattgcc
    24421 gccgcttgtt cttcggtgcg agatgaaagg tccgcattgc cagcggaaat ttcgctggtg
    24481 ccacgataaa tctcttccgc accctgtcga acagtcccta ctgtcatccc cagtgaatgc
    24541 tgcatttgct gtaaatgacg acttaagcga ccgatttcat tacgacccgc cggttcatca
    24601 ttcatcgtca gatcgccact ggcgattttt tcaatccgtt gtgcggcatg ttgcagtgga
    24661 cgaatgacga tccgacgtag caccataaat gttatcagcg tcatgaccag ggcaagcaca
    24721 aacgcgccaa tcatgaacat cccacccagg cgggtgcgct gatgggccag ttccgccagt
    24781 tggttggctc tggtgctacg tatcttaacg gctttgctca aaatatcggt ataagcatta
    24841 tccagcggtc ggatctgctc actttcatga ttgataatcg cttcaaacat gccatttttg
    24901 gcatatttca acataggttg catacccgtg atataagcct ggaagcgttg atttaattca
    24961 gtgtcgaggg cttcatcagc aggtgttttc accgaccgat tctgataagc acgataacct
    25021 tgctgcgact gtttaatctc cgattcggct tgcgcaatat ttcgcttcat tgcttccatt
    25081 tccgcaatac gactcgctgc cccggcctga atcatattga tccgggctga acgcaaaaaa
    25141 tctgaactgt tcgctaaccc ggtccgaatc tcaatctccg cagtaacatc acgctgatcg
    25201 cgatcggcct gccagaggaa atagccagcc agagcagaac ttaatgcgaa tagaactaag
    25261 ataccgccaa gaatgcaggc aaataacgga accagcctga tgtgatgcaa aaaacctaat
    25321 cgctgtgagg gagttgtatt cattaccgtt gtctctcgtc caggtttact cctgatagaa
    25381 agtcatcggc acttgacggt aattacttat gaccaaaaac ctctttgcgt cacattttta
    25441 caacaattat ggcagagaag aataatgcca ttggaatgaa gagagtaacg aatgattaaa
    25501 ctggctaacc cgaatgacga aaaattctgg aaaacagcac gcaatttcgt caggtgatta
    25561 ttttcgtccc taaggaaacc acataggatg ctcacgggtg cctgagactt tctgttttca
    25621 ggctaaccgg gaatcagaaa gacgaaggcc ccgaccgata tttctatcaa ccgaggccaa
    25681 cgaatgaaca cagcaattca gatcttagcc tcttacccgc catccggcaa ggagaaaggc
    25741 tatgaagcgc aaccctctgg tggtgtgtct gctcattatc tgcattacga ttctgacatt
    25801 cacactcctg acccgacaaa cgctctacga actgcggttc cgggacggtg ataaggaggt
    25861 tgctgcgctc atggcctgca cgtccaggta agggcaagcg cggggatttt ccccgcgcat
    25921 ctgactagca gattcatttt ctcatggcac cccttttaca cttcagttaa tgcatgtttt
    25981 tttcgatgca ctaatagaac catcattaca aacaaatttg ccgcctgccg tacagtgtga
    26041 gatcccccct ttcgaaccag aacagggttt gcgcccggca tcggcataaa aggaaacaag
    26101 cgttcctata accaagataa gtgccagttt tttcatataa cctccatggt ttatcgaatc
    26161 ttatgttcga ttttaacgtt atacgctggt ttattttgat
//
LOCUS       DAZKGF010000060        26008 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZKGF010000060 DAZKGF010000000
VERSION     DAZKGF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.060"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(29..4489)
                     /gene="mukB"
                     /locus_tag="ACHOQV_002333"
     CDS             complement(29..4489)
                     /gene="mukB"
                     /locus_tag="ACHOQV_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFP9630700.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPYQAS"
     gene            complement(4489..5193)
                     /gene="mukE"
                     /locus_tag="ACHOQV_002334"
     CDS             complement(4489..5193)
                     /gene="mukE"
                     /locus_tag="ACHOQV_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFP9630701.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5174..6496)
                     /gene="mukF"
                     /locus_tag="ACHOQV_002335"
     CDS             complement(5174..6496)
                     /gene="mukF"
                     /locus_tag="ACHOQV_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFP9630702.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6493..7278)
                     /gene="cmoM"
                     /locus_tag="ACHOQV_002336"
     CDS             complement(6493..7278)
                     /gene="cmoM"
                     /locus_tag="ACHOQV_002336"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFP9630703.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7414..8193
                     /gene="elyC"
                     /locus_tag="ACHOQV_002337"
     CDS             7414..8193
                     /gene="elyC"
                     /locus_tag="ACHOQV_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFP9630704.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKSSSGEPRQE"
     gene            complement(8170..9063)
                     /locus_tag="ACHOQV_002338"
     CDS             complement(8170..9063)
                     /locus_tag="ACHOQV_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFP9630705.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDTSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9217..9963)
                     /gene="kdsB"
                     /locus_tag="ACHOQV_002339"
     CDS             complement(9217..9963)
                     /gene="kdsB"
                     /locus_tag="ACHOQV_002339"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP9630706.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9960..10142)
                     /gene="ycaR"
                     /locus_tag="ACHOQV_002340"
     CDS             complement(9960..10142)
                     /gene="ycaR"
                     /locus_tag="ACHOQV_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFP9630707.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10194..11426)
                     /gene="ycaQ"
                     /locus_tag="ACHOQV_002341"
     CDS             complement(10194..11426)
                     /gene="ycaQ"
                     /locus_tag="ACHOQV_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFP9630708.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLGDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPEWNDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRAGWEIDPVA"
     gene            complement(11463..12449)
                     /gene="lpxK"
                     /locus_tag="ACHOQV_002342"
     CDS             complement(11463..12449)
                     /gene="lpxK"
                     /locus_tag="ACHOQV_002342"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFP9630709.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     NAGQTLVMTEKDAVKCRAFAEGNWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(12446..14194)
                     /gene="msbA"
                     /locus_tag="ACHOQV_002343"
     CDS             complement(12446..14194)
                     /gene="msbA"
                     /locus_tag="ACHOQV_002343"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFP9630710.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14231..16495)
                     /locus_tag="ACHOQV_002344"
     CDS             complement(14231..16495)
                     /locus_tag="ACHOQV_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFP9630711.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLASLTVFACVLVFIPF
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAMWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVAHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSQLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16702..16986)
                     /gene="ihfB"
                     /locus_tag="ACHOQV_002345"
     CDS             complement(16702..16986)
                     /gene="ihfB"
                     /locus_tag="ACHOQV_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFP9630712.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17146..18819)
                     /gene="rpsA"
                     /locus_tag="ACHOQV_002346"
     CDS             complement(17146..18819)
                     /gene="rpsA"
                     /locus_tag="ACHOQV_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP9630713.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18930..19613)
                     /gene="cmk"
                     /locus_tag="ACHOQV_002347"
     CDS             complement(18930..19613)
                     /gene="cmk"
                     /locus_tag="ACHOQV_002347"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP9630714.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19786..20550)
                     /gene="ycaL"
                     /locus_tag="ACHOQV_002348"
     CDS             complement(19786..20550)
                     /gene="ycaL"
                     /locus_tag="ACHOQV_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFP9630715.1"
                     /translation="MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(20719..22002)
                     /gene="aroA"
                     /locus_tag="ACHOQV_002349"
     CDS             complement(20719..22002)
                     /gene="aroA"
                     /locus_tag="ACHOQV_002349"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9630716.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQDNYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNMMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRIAMCFS
                     LVALSDTPVTILDPKCTAKTFPNYFEQLARISQAA"
     gene            complement(22073..23161)
                     /gene="serC"
                     /locus_tag="ACHOQV_002350"
     CDS             complement(22073..23161)
                     /gene="serC"
                     /locus_tag="ACHOQV_002350"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFP9630717.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSNFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(23360..24052)
                     /locus_tag="ACHOQV_002351"
     CDS             complement(23360..24052)
                     /locus_tag="ACHOQV_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP9630718.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFIILALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESVDYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            24182..25942
                     /gene="ycaO"
                     /locus_tag="ACHOQV_002352"
     CDS             24182..25942
                     /gene="ycaO"
                     /locus_tag="ACHOQV_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFP9630719.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELAGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         6305 a         7080 c         6325 g         6298 t
ORIGIN      
        1 aaaaggcggc attgctgccg ccttcatttt aactcgcttg gtaaggcgct tcgtcacttc
       61 ctggaagcgt ttcagggagt tgcggcgcaa atcctcgcag gccgacgacg tgaacgtgtt
      121 cggtattctg gaagacttta cgcaccagtt tataggtggt gcctttttcc ggactgatat
      181 tttccggcgc tgcgatgatg agctgcattt gcaaacgttc acacaattca aacagcgtgg
      241 cgatagaacg agcatccagt cgcgctgctt catcgaggaa cagcaggcgg caaggagaga
      301 tatctttacc gcgcaggcgg cgagattcat cttcccagct ttgtaccacc atcaccagaa
      361 tcgacatacc ggtaccaatc gcctcaccgg tcgacagagc gccagattct gcgcgcagcc
      421 agccatcaga accacggtta acctcaactt ccatttccag atagttgcgg taatccagca
      481 gttcttcgcc aatggtctgc ggcgtgcgct gccccatatc aatctgcgga ttaagacgct
      541 gatacaattt cgccagcgct tccgagaagg tcagacggtt gctgttaaac agatcctgat
      601 gctgctcgtg ctgttcagag agcacatcca gcagcatggc gtgcgtttca cgcacgttca
      661 cattcagacg tacgctgttt acctgaccga acgatacgtt ctgcaaaccc tggttgagca
      721 tacggatacg gttctgttcg cgctgaatgg ttttgcgaat aatgttcgcc acgctgcggg
      781 aactgatcgc cagtttctgt tcacgggagg ttaactcttc ggtcagacgg ctaagttcga
      841 tctccatctg ttcgatagct tccaccggat catcggtacg aataatatcc tgacgaatac
      901 gttcacgcag atgctgataa accgccacga agaactgaat tttacgttcc ggacgttttg
      961 gatcttccga catccgcagc acgtcgcgca gatgttcgtt atccgccacc gccagacgca
     1021 gcgcacctaa cgccttatcc gacatggaac gcaaatcatc agcggagaga taagccagct
     1081 cacgacggtg taagcggcgt tcaacgccgt tatctttcac catgcgcatg actgcacacc
     1141 agcccgcttt cgcggtcact acctgctcgc gcatctcaaa gtaatcacgc tccagcttgc
     1201 gcagtttgcg ggtcaggttg tccatctccg cttcgcagaa ggtaagcgct ttttccagtt
     1261 gattgcggcg tgaacggtta ttgctcagtt gcgcgtgcaa ctcgtcacgg cgaatacgcg
     1321 cccgctcttc tgccccgcta tcagcacgca cgccgatatc ctgcaattca cgttgcagat
     1381 cgttgagtag ctcttttttg gtgtcgtaag aacttttcag cgaagccagc acctggttgt
     1441 actgactcaa ctgcgctgcg tgtccgcgca acgcttcacg cgcgcgggta cgctccgctt
     1501 ccgcctgttc cagacgttca cgcagttttt cgttgagatc gctgttaccg ctcagcattt
     1561 ctgccgagtc agagtagcta aagtgcgcac gacgctgcac cacttccgtc agggcaaacg
     1621 cctgctgacg ggcatcgcgc tgcatctgct gagagtacgc gtaatcttct tttaactgtt
     1681 cgaactgttc cgggtcgctc tgcaataccg aaacgatcgg ttccagtttc gccagttgat
     1741 tgccaaactg ctgaacaaaa cgcgcggctt cctgggcttc atccagacgt tcgcggattt
     1801 catcgacgcg atccgccagg ctgtcatcag ccaacaggtt gagacgcggc agaatgcggt
     1861 tcagcgccgt aacgccctct ttcgcctgct caaattgaat acgctgctgc tggttatcat
     1921 tttcatgatt actcagcgcc cgctccagtt cgacgcgacg gctgttcagt tgacggattt
     1981 cggcttccgg atcagactca aacgccaccg ccagatgact acctataaag cggctgaacg
     2041 cctgatgcag acgctgggtt ttctgtacat caaaagagag cgtggcgaag cgttcggaaa
     2101 gcacttcacg ctcggcatgg aggctttcaa tacggctttc acgcgcagca cgaccaaaca
     2161 gcggaacttc cggaaaacgt gagtaacgcc actgacgatc ggcgattttc accactaccg
     2221 ctttttccag ctcatcaacg ctgaacacgc tgtcatcgaa tgactgcgga tccccttcga
     2281 tcagatagag atcttccggg caatcggtca agccttccag gtgttcggtt acctgtgaca
     2341 gatctggcac cacgatggcg tggcgtgacg ggccatacag tgccgagaag tacggcgcat
     2401 cttccagact cacgtcgtca taaatttcgg acagcagcac gccgccaaaa cgctccgcca
     2461 gcgcattcaa acgctggtct tcagaaccac ctggctggct taaacgttcg atctcttcat
     2521 cgacggcgtt tttgcgcgcg cctacttcat cgcgttcaac aatggcttcg cgctcacgct
     2581 ccagcaactg ttgcagatat tcagtgacgt cctggctgga ggtgaactct tcgccgcact
     2641 gttcgctcaa ctggttgaga ctgttttgcg ctgccagcca aaccggtgca cgctgcatca
     2701 aactctgaat gcgagactgc agctgttcct gttcctggcg cagtgccatc cgctcttctc
     2761 gggcgttaga gacgctatcg gaaagagagg caatgcgtgc ttccagctcc tgatgcagag
     2821 cttccagttc atcaatatca aaattcttgc cctgacgttt gcagaaatct gccagcagac
     2881 gctcagcttc ttgctgctca cgcagacgct gttccagttc gcttaagcgc atccgcaacg
     2941 gctgaacctg ctctgccagg tgacgctgat cgaccccttc gcgcaatagt tcgcgagcga
     3001 catcccacgc ctcgttacgc gccagcgggc cgttgattgc caccaccagc tgataagcct
     3061 gctcaaactg gctgtgcgcg gtttgcgcca tgctcatttt ctgctcaaga gagagcattt
     3121 tttcggtcgc ttccagctct ttcgcctgga aggtttccag ccattcggca gcgctgtcgg
     3181 cggttaagtc cggcagatgg cacagttctt tggcacgatt aagcgcagca atcgcctggt
     3241 tatactggat cgcgcgcgtt tgctggacgt ccagcgcctg ctggtagtca gcaagctggc
     3301 ttttcagctc gtccacttcc agttcggcag cttccgcacg agcctcgttc tcttcctggc
     3361 gttcgatggc ttctgccacc acttcgtttt gctcttccag acggatctgc agctcatcga
     3421 gatccgcttc gtagcgttcg atcttctcct gctgacgcag tgcggtttgc accaggttca
     3481 ggtgatcgct ggccgcctga taatccgctt ccagatcgcc ttcggcaccg ttgtgctcag
     3541 ccagctctcg cgccatgtcg acgtgtttgt actgctcagc cgccagttgc tgacgcgaag
     3601 tatgtagctc gcgacgaaac tccagggcct tatcgagatg aacacggcgc tcgttggcgt
     3661 gacgcatgta gtccgccgcc acgtagttgg tggcttcgct gatcagatgc ttaaacaggt
     3721 cacggtctga ctgggtgaca cgaatcgctt ccagcgtcat gcggttttca cgcagcgccg
     3781 cttccatgtc ctggaacgct ttccgcaccc cactgttttc cggcaacagg tagtcgcgca
     3841 gagaacgagt aatggcgctg gagatcccgc catacagcga ggcttcgatc agacgataga
     3901 atttactacg gtcagacgct gagcgcagac gacgcgcgat gatgcccaga tcgaacatca
     3961 gcgagtggta atcggtaatg gagttgaact gtttaaactg cacgccttcc atcgcctcga
     4021 gcttgtcttt cagctcgtta agcggcagca cgcgcgcctg gcgttcgttc agagtttcgg
     4081 tcaccagttg tgtcggctgc accgacatcg gcaggccctg aatggcaaat ggtttgatat
     4141 ccactttacg atcgcgtccg gcaacctgtt gcagacgcac accgaccact acacgctggt
     4201 ggcgcgagtt aatggtgtcg agcatcgaat aacagacacc cgctttcagc ttaccgtgca
     4261 gacctttatc gcgcgaaccg ctggtggccc cggcttccgt agtgttacgg aaatgcagca
     4321 gggtcaggtc ggggataagc gccgtaacga acgctgccat ggtggtggat ttacccgccc
     4381 cgttaccgcc agaaagcgtc gtgaccagct cgtcaaggtc aaaagttcgg gcaaaaaagc
     4441 cgttccagtt aatcagcgtc agtgagcgaa atttaccgcg ttcaatcatt attcttcctc
     4501 tccgctatct ggctgactct cttcggtttc atcgttgagt tgcagatgat tttcaatcgg
     4561 cattgcttcg ccatcacgaa tcaggcgacg ctgtgcttca cggggatcgt cgccagcacg
     4621 cacatcggct ccgaagcgga acaccgattc ggtaatgcgg aacttgctgc tgtcgtggcc
     4681 cataaaccac accataccca aacgacgcag acggttgagc gaagaacgta ctttctcctg
     4741 caacttctgc cggtcaacgt ctgaaccggt tgaacggttg ttcaccagtt tcagcagttt
     4801 cgcttcatcg gccagggtga gcagttcgtc gtacagttcc tgctgggtga aaatcccctc
     4861 attcgccagc cgttccgggc tgagatagag ataacagagg attttcccga ccatcatatc
     4921 cagttccgac aagacggaac gagggatcag cgtggtggaa cgtgggcgta gatagaagaa
     4981 cccttccggt gcacgaataa gctcaacgtt ataacgcgcg taaaactctt ccagatattc
     5041 ctgaaaatcc atcaggaatg catgattatc cagttcgtcg aggccaatat gacggcctga
     5101 acgtaaggcg ctatccagcg ccggaaataa cggattcgcc agcgcctgcg ccagcttaac
     5161 cggcatcact tgttcaatat ttgtcgatga catgcgcctg taccttggct ccgtaatcat
     5221 taatcggctg ccatttcgct ggcagtccgg tgaaatctgc ttgcgctacg ccaagacgta
     5281 ccgcctgatc aataacaatg cgcgcaacgt caaagtgacg tgcacgcgga tactgtgaca
     5341 gatattcgcg taccaccaga ccaagatcca acggcacttg tctggttttg tacacggcaa
     5401 gttgttcttc gatgatcgcc gccagctgtt cgcggatctc gttaaactct tcgtactcca
     5461 gatcctcagg aagttcccca gtcacttctt catcgcgcag taccatctct tcgtcacgca
     5521 tatccagcag acgatcggca ttggcataag ttagcgccca cggctcatca aaataggttt
     5581 gtaccgactg acgtaaccgc tgagcaaaga cgcggttttt atccatatcg atcgcagtac
     5641 ggataaattt gtgtacgtgg cggtcgtagc caatccacaa gtctatggat tgctggcccc
     5701 aactgataat acggtcgagt ttgctctgca gatcgaacac cagacgatcg acaaaatgca
     5761 ggtcgtcatg ggtcatcgtc gcatcctgaa tgcgcaacag attagcctgc aatttatcgc
     5821 ctgccgcttc cagcgtatcc tgcaattcac gcagcgttcc ggaagtttct gaaagcaaca
     5881 attcacagct ggaaatcgcc gcccgccagt ctttgttcaa caactgggcg atatcatcct
     5941 tcacctgctg ctgctgttcg tccatcagac gttgcgtcag gtcgatactg tcgaaaattt
     6001 ctgctaccga atatttcagt ggcgcataaa cattacggtg ccagtgaaat tcatcaccgc
     6061 cctcttcggc ggcatctgcc gcgcgtttga gctcacccgc cacaatcgac aactgcatag
     6121 aaaggcgcag cgtagaaaac tcgcgctgac ggatgtaata gtcagtaatg ccgatgccga
     6181 gcggcgtcag acggtaaatt gcgttccctt ccgcctgctc gctggtaaag cggttcagca
     6241 gacgttgacg caccatgtcg ttgatcgcgt tgttggcgcg cacgccgatg gtttcgctgg
     6301 tttgctcaaa cgcatcactc acatggcgga atgcatccac cagctcgcct tcactcatct
     6361 caccatccag acgctcgccg ttcagcgtgg caaccgccag cagaaaagag agtcggtcta
     6421 ccggcagcga gatggagaag tcattttttc tggcccaggc aaccagttcg gggactgtct
     6481 gggaaaattc actcatactt tatccttgct ctgcggtttg cgcgcggtga catgaatata
     6541 acgccccagg gtaatatacg gttcctgacg gcaataacgc gtttctaatt caagtaatgc
     6601 ttcatagcag tcgcgctgct ggtgcttctc gcgcagataa tcatgaaaca cgcgaacgcc
     6661 tgtcttaccc ataatttgcc aaccagcttc ttccagccac agataaacct gcgccgggtc
     6721 gcgtggataa tctggcgaaa gcgtccgttt tttctttttc ggcattcccg cctgcacgta
     6781 atcaaaattc ccggcgacca tgttatgcat cagcaagccg tgcgcattgt agaacattaa
     6841 cgacaacaca ccgcctggac gcaacactga ccacagggtc tgtaatacgc tgcggggatc
     6901 ggccacccac tcgagcaccg catggaacaa tatcagatca acgggcgttt ccaaatgtga
     6961 agcaacatcc tgagcggcgc aatgtataaa ttgcatgttg tcgctcacac cttttgcttc
     7021 tgccgcctgt tttgcgcggt cgatcatctg cgcagaaaga tcgcataaaa tgacctgatg
     7081 cccgcgctca gccattttga ttgcggtctg cccttctcca ccgccagcat ccagcacacg
     7141 cagtttttgc gggcccattt ccgccagcac gcgatcgaga tcctgccaca gaatagcctg
     7201 tcgaagctgc cctttggtgg tgccgtaaat gttacgggaa aacttttccg caatatcatc
     7261 aaaattgcga tcctgcattg ctttctccac cggttggcta agacgttttc tgataaagac
     7321 gctattttgt cacagccacg gcgagaatga acccgtctgg tgtaatatcg ctggcgatcc
     7381 cttgctatat ggttaaaaaa ggaaccagaa aggatgcttt ttacactgaa aaaagtgatt
     7441 ggcaatatgt tgctgccact tccgctaatg ttgttgatta ttggcgctgg cctggcgctg
     7501 ctgtggttta gccgttttca aaaaaccggg aagattttta ttagcattgg gtggctggcg
     7561 ctcttgttat tgagtttgca accggtagcc gaccgcctgc tgcgtcccat cgaaagcacc
     7621 tatccgacat ggaataattc gcaaaaagtg gactatatcg tggtgctcgg aggtggttat
     7681 acctggaacc cgcagtgggc accaagctcg aatttaatca ataacagtct gccccgcctg
     7741 aatgaaggta ttcgcttatg gcgcgaaaat ccgggatcaa aactgatctt cacgggaggc
     7801 gtagcaaaaa ccaatacggt gagtacagcc gaagtaggtg cccgagttgc gcaatcgctg
     7861 ggcgtgccgc gcgagcaaat tatcactctg gatttgccaa aagataccga agaagaagct
     7921 gcggcagtga aacaggcgat tggtgacgcc cctttcctgc tggtgacatc cgcgtctcat
     7981 ttgccgcggg cgatgatctt ttttcagcag gaagggttaa atccgctgcc agccccggcg
     8041 aatcagttgg ccatcgactc gccgcttaac ccgtgggaac gggcaatccc ttctccggtc
     8101 tggttgatgc atagcgatcg tgttggctat gaaacgcttg gccgtatctg gcagtggcta
     8161 aaaagttcgt caggcgagcc acggcaagag tgattttgat gccagatcga agttgcgccg
     8221 actaaatcgt ccggtattaa ccagttgcgc aacttcatcc cataacacat acaaccaacg
     8281 ccgccagatg aacgactccg ccactggcgc gcgttgcagg taactccaga gcaaatcttc
     8341 cgccagagaa gtatccatta gtcggaacag ttcgtattcc cgcggtgccc aaagcattag
     8401 tcccggcccg accatcgcca gtaactgatc gctgcgcgaa tctttcagca tgctgcgtaa
     8461 acagaagtta ccgtgaatca gcacacaatt gtcgttaaag ccttcaaata atgccgggag
     8521 acattcgcga gtacgaaaca ggatccgctt atcctgcatc gttagaccgg tgttattgaa
     8581 ctgattgagc gtggtccata gcacttcaac atgttgccgg taccatgagg gccagaaatt
     8641 ttcctgagta ttgtcgaccg cgccgacgca accgcgactg tcctgacggt gccaggccag
     8701 taaggcttca acgatttgat ctttgagttg ttcccagcgt tctggtgttc gggctggtgc
     8761 ctccaccgaa acaccacgca tccgctccag tagcaggaca tcggggccgg ggtgttcttc
     8821 atgcgtcatc acaccataaa tagtcggcat acggacagtc ccacttcttg ccagcatggt
     8881 ggttttccat gccagttgtc gggcttttcc gggcgtacta aagcttcttg ccattaacgg
     8941 cattgggttt ccctggctgt catacaaagc ccacaacgcc gtatccgctt tttcattgac
     9001 gcactcaata cgactgagtt tttcgcccag taaatggctt aattcggcac gcagctgttc
     9061 cattcagatt accctcataa aagctactgc tttcataatg agcgccaata taaaagatgt
     9121 cacctgaatg agatcaattg ttgaaaagaa aaattgagga aaatcgtaac ggtacgacac
     9181 tcctcccaaa attggctgga gtgtcgtgaa gtgaaattag cgcatttcag cgcgaacgcg
     9241 ctcgagatct tcaggggtat ccacacctgt gccaggaact tcctgggcga cagcaacatg
     9301 gattttttcg ccgtaccaca gaacacgaag ctgctctaac atttcgatgt gttctaacgg
     9361 acttggctgc cagttgacgt aacgacggat aaagcctgca cggtagccat aaataccaag
     9421 atgacgcagg aagttatcgc caacggtttc aaggcctttt gcaaaacgat cacgatccca
     9481 aggaatggtg gcgcgagaga agtacagtgc atacccttca gcgtcgagga ccactttcac
     9541 cgcattcggg ttaaacgctt cctccgcatt gtggattggc accgccagag tcgccatacc
     9601 cacctgacgc tgagcgaggt tatcagcaac ctgacgaatg attgtcgcag ggatcatcgg
     9661 ttcatcaccc tgcacattaa cgatcacagt gtcgtcgctg aatgcgcatt tttcgacaac
     9721 ttccgccaat cgttctgtac ctgactgatg atcggcgcgc gtcatacata cttcgccgcc
     9781 agcggcttca acagcgcggg caacatcctc atgatcggtt gccacgatga tgcgctcggc
     9841 acctgattcg cgcgcgcgtt caagaacatg aacaatcatg ggtttgccgt taatatcaac
     9901 caatggttta ccgggcagac gcgtggacgc gtaacgcgcg ggaataatga ccacaaaact
     9961 catgatttac tctcatcagc agtcagcacg cgggcttccg tttccagcaa cactggaatg
    10021 ccatcgcgca gcgggaaagc caggttgtcc agtttgcaaa taagctcttg tttttcctgg
    10081 ttataccaaa gttttccgtt gcaaaccggg caggcaatga tttcaagcag acgatgatcc
    10141 atagttcctc cagatggatc gggttatgaa tgcataaatc ttatcataat catttatgcg
    10201 acggggtcta tttcccagcc ggcgcggcaa tcagtaaaga ggccttgcgg gcagcgtcct
    10261 aatgtcaccc gcgttgcctg ctgccagtta gcgaaatcag taatcgcctg acgtaaccct
    10321 ttttgcagcg ttgtcgttgg tttaatgcct tcttgtaacc acagagagat aacttcaagg
    10381 atgcctgtct ggcgatgcat tttggcatcc attcgcccaa ctaattgccc acgatgtaat
    10441 aacggcagaa caaaatagcc atactggcgt ttcggcgctg gggtatagca ctccagccgg
    10501 tagctaaaat caaaaagctg ctctgcgcgt ttgcgatccc agacaacagg atcaaaaggc
    10561 gaaagtaccg cgctgtgcgt tgcagtgagc tttccggcca gcgcacgctc gagtagcggc
    10621 agcaaatcag catgcagcca aagattgccc aatttttcaa catgcacagc aatgatttgc
    10681 tgctgttcag ccctcgcttc acgccacgcc gccagtgccg gacgttttag ccggtagtaa
    10741 tctgccagcc actgttcgcg aaatataccc aggctacgcg cactgttatc cagcatgatg
    10801 atttctgctt ctgcttgcga aacgagatcg cgctcatcat tccactcagg catgacacgg
    10861 tgagttaaat cataaacgcg ctggaagttg cgccgttcaa tcaccatcac ctttccggca
    10921 gtaaataaac cttccagatg tcgtttatgc ggcttccact cccaccagcc gctcgcccct
    10981 ttacgaggat gctcaaaatc ggctgaacgt actggcccct tatcatgaat atgctgaatt
    11041 aactgtgcaa tttccgccgc atgttcctgc atccaggcgt ctttatattt ccagcccatt
    11101 ttttcaggtg ccagcatgcg gtggcgaata agacgaaaat cgctacgcgg cataaagcag
    11161 gcttcatgcg cccagtattc cattaattcg ccacgcgcca gagactcatc cagccactgg
    11221 gcaggataat ttcccagacg actgaaaagc accagatatg gactacgggc aacaatattg
    11281 atggtatcga tttgcagcaa ggacatgcgg gagatcgttg ccggaatatc ccccaacgac
    11341 gctcgacggc ggggtttgtt taacaggcct tgcgcggcga ggtgaagatt acgcgcatca
    11401 gcaagagaga ggtgcggcag cgacattcat gactccatca atcgaacgct gccgcggcgt
    11461 aactagttgc cagaagccag cgaggttagt tgcgcaagca gtttcgctgg ttcatcacct
    11521 gaaagctgtg cgtcaacagg caaataccac caatttcctt cggcaaaggc ccggcatttc
    11581 accgcatctt tttcagtcat taccagcgtt tgcccggcgt ttaccaacgc gctgacatcc
    11641 gcatggttca aagactgatg atcggccagc ggtacacatt tttccggttg tacgccgcac
    11701 atcttcagcg tggcaaaaaa gcgcggcgga tgtccaatcc ccgccatcgc cactacatgt
    11761 tcaagctgag caacgtcaca acgcgtaccg gtacgtaaat tcaccgcctg acccggcagc
    11821 agatgcatgg ggatttcacc gctgcggggg acaccgccgt tgacgattac cgcatcaacc
    11881 gactttaagc gccccgctcg ctcacgcatt ggccccgccg gcaaccacca gccattgcca
    11941 aagcgacgca caccatcaat aacgacaatt tccacatcac gcgccagacg ataatgctgt
    12001 aaaccatcgt cggttacgat gatctgcaca tcagggtgtt gcgccagaat ggcttttacc
    12061 gcgtcagaac gcacgggaga aaccgcaaca ggcgcgccag tgcgttgata aatcaacaca
    12121 ggttcatcac ccgcctgtgc tgtggtggta tctgccgaca ataacagcgg ataagattca
    12181 gccttaccac catatccccg cgatacaacc cccacgcgaa taccgcgctg ttgcagctgt
    12241 tccaccagcc agacaacgac cggggttttt ccgttgccgc ctgcggtgag attaccaacc
    12301 acgacaaccg gtacgggggc acgccaggcg cgcttcagtt ttagtttata gcaaagacgg
    12361 atcgcgccac tcaccaggcc atacaaccag gagagtggca gcaatagccg ccataaaggg
    12421 gattcaccag accagatttt ttcgatcatt ggccaaactg cattttgtga agttgcgcgt
    12481 aaacgccacg gtgctcaagc aaatcgttat gcgtaccgcg ttccacaatg acaccatcct
    12541 cgacaaccac gatttcgtct gccttttcaa tggtagacaa gcggtgggca atcaccagag
    12601 aggtacggtt tttctgcaac tcatccagtg ccgcctgaat cgcacgttcg gattcggtat
    12661 ccaacgccga ggtagcttcg tccagaatca gtatcgggct atcacgcaac aaggctcgag
    12721 cgatagcaat acgctgacgc tgaccgccag agagcagcac gccgttttca ccaatcactg
    12781 tatcgagacc gttatccatc ttattgatga agtccatggc gtaggccata cgcgccgctt
    12841 cttcaatttg ctcacggctg tactgttcag tccgtgcgta agcaatgttg ttagcaaccg
    12901 tatcgttaaa cagatggaca ttctgcgaca ccagagcaac ctggttacgt aacgacgcca
    12961 gggtatactc gcgcagatcg tgaccatcca tcaggatttc gccttcatca atatcgtaaa
    13021 aacgcgtgat caggctggcg atggttgatt tacctgaacc agagcgtcca accagagcaa
    13081 ccgtcttccc tgccggaatt ttcaggttga tgttacgcaa tgcgggcaca tcacgtcccg
    13141 gataagtaaa ggtgacattg cggaattcca cgtcgccagt cgcacgctcg atcacgcgct
    13201 taccttcgtc tttctcctgc tcactgtcca gaatggtaaa cagcgtctga caagccgcca
    13261 taccgcgctg gaactgggcg ttaacgttgg tcagcgattt cagcggacgc ataagtgcaa
    13321 tcattgaaga gaaaacaacg gtaatcgtac cggcagtcag gctatccatg acacttggga
    13381 agcttgccgc atacagaaca aacgccagcg ccaaagaggc gatcagctga atgatcggat
    13441 cagagatgga agaggctgaa accattttca tcccctgaag acgcattcgg ttgctgactt
    13501 tatcgaagcg tttcgtttcc acttcctgac caccgaaaat caatacttct ttatggcctt
    13561 tcagcatttg ttcggcgctg gtggtcacct gccccatggt gttctgcatg tttttactga
    13621 tgttgcgaaa acgcttcgat acaacgcgaa tcgcaatcga aacaatcggt gccagcacaa
    13681 tcaaaatgat cgacagttgc caactgtaat agaacatcat gatgaacagg ccgatgatcg
    13741 acgcaccttc acgtacaaca gtaatcagtg cgccggaaga agaagaagcg acctgttcgg
    13801 aatcgtaggt gatacgtgac aacagcgtac ccgttgactg tttgtcaaag aatgaaaccg
    13861 gcattcccat catgtgacca aacaggcggc gacgcatagt cattaccacc tttcctgata
    13921 cccaggagat acagtagctg gagatatagc tggtgatacc acgtaaaatc atcagcccga
    13981 tcaccaccag cggcatccac atcagcacgg agcgatctgt tttaccaaag ccatcatcaa
    14041 gaagtggctt aaggagcgat aacatgaagg tatcgctggc tgcgttgagg attaacgcta
    14101 cgcccgccac gatcagaccc gctttaaaag gcgcaatggt tggccacagt cggcggaatg
    14161 tctgccacgt agagagatct ttgtcgttat gcattcaaaa aaccagcatt tgttgaaata
    14221 gccgcatatt ctacccgtta tccactggca cgccaaacca ctgatgatac caacgaggta
    14281 aaatttgctc tctgaggctg ctaatccgcc agccttgcgc tgaaaaattg accgttattt
    14341 gaccctgatg tggagtatca agccacttat aaccctgttg ttgatagcga tgtttaactt
    14401 tgcttgaggg cagtcgccat gcgttatagc gcgatgccga tgcgagtgcc acttttccat
    14461 tcactcgctg aattaatggc aacgatgatg aggtattact gccatggtga ggtacctgaa
    14521 gtaatgttgc ctgcatttgc tgccagtaac ggcttagcat cttttgttca gcgggggctt
    14581 caatatcacc ggttagaaga atgctattcg tcccgtcatc aatcttaacc acacaggaat
    14641 ggttatttcc tttatcgttg ctaccttgta aaggccagtg cgcgctgaaa cgcaatcctt
    14701 gccattgcca cgcttcgcca cgcacacagg gctgatgatg ttcccagttt aactgacttc
    14761 ggatccataa catcggccat gtgtgcaata ttgagtccag ccctccccgg tgatccagat
    14821 gttcatgact caaaataacg ccttccggtt caagattatg ccagtggagc caggggataa
    14881 tcagttgttg cccactatcc ccttcgggcc aggccagtcc cgtgtcatag agaatcgctt
    14941 tgccgtttcg cgcaatgacc attgccagcc cttgcccgac atcaagcatg tacacttgcc
    15001 actcgtcagg tcgaggtttt tgccacagcg gcaagcacat cagcaagcct acagccacac
    15061 acattgctgg caacgttcgc caggcgttta atcgccatac caccagtaag aaccatgggg
    15121 aaaatgttag ccattgccaa cgttcagcaa tgttgatcca cccttccggc aaactcttta
    15181 acccccagaa aagtaaagcc aaagaccggt cggcaagaaa ccataaccct tgttccagaa
    15241 taaacggccc gcttaaatgc gcaaccatcg cggcgaggat caacggaacc gtgataaatg
    15301 tcaccagggg aattgcaaat agatttgcaa taaacgaggt cagactaatg ccatgaaata
    15361 tgacgatttg cacgggcata agcaggagtg tgattcccag ttgtaaatga atgagggaaa
    15421 caactgcacg caataccggc ggcagttgcc actccggaca gggaaaccac tggtaccaaa
    15481 aaatcagtgc cgcgaccgca gcggcagaga gccataaact ttgcgagaga atggcaacag
    15541 gatccatcag caaaattgcc gccagacaac atatccatac atcccagcca ctccattgtc
    15601 gcccacttaa cttaagcatt ccccacatgg caagcgccac cacggtacgc aatgcaggag
    15661 gttgcatccc agtcagccag gcataaaaag cagcacagca gattccgcca attaatggca
    15721 tttgccagtg gatccagcgc ccaggcagaa aaacttgccc accgcgaatg agtccggcag
    15781 ccagcaacgc cgcaaaagcg atatgcaatc ccgatatcgc cattaaatgc gccgttccag
    15841 tatcgcgcat gatatttttg atttctttgg gaacggataa ccgttccccc atacctaaac
    15901 caagaataac cgcgttccac atatagggtt gcagcgttgt ttgtagtgac gccagatact
    15961 gtgcacgcag gctacaattc ggttcaatga cacttgcctg cagaaagcgg ccggtgagcg
    16021 gctgatgctg ggcaatggca taacgctggc tatcaaagcc gccatcatta agttgaccat
    16081 gaactgcacg aactttgagt ttcattgacc acagttgtcc agcacaaacc gcttgcggaa
    16141 gatattcgcc atacagcacg aggcctggcg cagggaatat acgtcgacct tgtagatgag
    16201 taatttgacc gtaatgagtg gtcataccgt cagtagcagt gatctcaaca attgcatcct
    16261 gcgtcgcgcc agttaaggtt tctcctgccc acaaaatttg ctttgctgct aatatgcccc
    16321 aaacgaaaaa cagcaacgtc agcgcgatat aacggacagt tttaaaaggg ataaatacca
    16381 gtacgcaggc gaagacggtc agagacgcaa gggttaatgt cccaggcaat tggggcaaaa
    16441 tcagcaacgg aaaaattccg caaattatgc atacgccgac tgtcgttatt ttcatgcacc
    16501 cctccctgtt tatggaaagt gtgcaacttt gttgagcggt tgtctggtgg gagttttgcg
    16561 ttccccgagg cacgttcaga agaatttaac gagagggtga aaactgttca aaactctgcg
    16621 aggctgcttc cagttccaga ttagagaaat gaaaaaagca cccgacaggt gcttttctcg
    16681 cgttcaagtt tgagtaaaaa cttaaccgta aatattggcg cgatcgcgca gttctttacc
    16741 tggtttaaag tgaggaacgt attttccttc cagttctact ttatcgccag tcttcggatt
    16801 acgtccggta cgtggtgcgc ggtagtgcaa agagaaactg ccgaaaccgc ggatttcaat
    16861 acgctcgccc tgcgcaagag tcgaggccat atgctccagc atctctttta ctgcatcttc
    16921 aaccgtcttg gcgggaatgt gcgattgctg ggtggcaagt ctttctatca attctgactt
    16981 ggtcatgatt cctccggttc cttaaaggca aatttagcgg ctgctgttgc tgcattgatt
    17041 aagggcggcc gtagccgccc ttagtgcttg attacaggac gaaacctgca atctgtcaag
    17101 taaactcaac aaacttcgga ataaaaatcc cgaagagtca gagaattact cgcctttagc
    17161 tgctttgaaa gcttcagcca ttgcgttgtt ggagaagttt gcatcttcct gtttgttaac
    17221 agttgcgatt gcatctttct cgtcagcttc gtctttcgca cgaacagaca ggctgattgc
    17281 gcggttttta cgatcaacgc cggtgaattt agcttcaact tcgtcgccaa cgctcagaac
    17341 cagggtagcg tcttcaacgc ggtcacggga tgcttcagaa gcacgcaggt aaccttcaac
    17401 gccgtcagcc agttctacgg ttgcgccttt agcgtcaact gcagttactt taccggttac
    17461 gatagcgcct ttcttgttca gagcaaccca gttgttgaac ggatcttctg cgagctgttt
    17521 aacgcccagg gagatacgtt cacgttctgc gtcaacctgc agaacaactg cagcgatttc
    17581 gtcgcctttt ttgtattcac gaactgcttc ttcgcctgca acgttccagg agatgtcaga
    17641 caggtgaacc aggccgtcga tgccgccgtc caggccgatg aagataccga agtcagtgat
    17701 agacttgatt ttaccttcaa cacggtcgcc cttgttgtgg gtttccgcga actgctgcca
    17761 cgggttagct ttgcactgtt tcagacccag ggagatacga cgacgttctt cgtcgatatc
    17821 cagaaccata acttccacta catcgccaac gttaacaact ttggacgggt ggatgttttt
    17881 gttggtccaa tccatttcgg aaacgtgtac caggccttca acgccttctt cgatttcaac
    17941 gaagcagccg tagtcggtca ggttggtcac gcgaccagtc agtttggtac cttccggata
    18001 acgtttagcg atagctaccc acggatcttc gcccagctgt ttcaggccca gggatacacg
    18061 ggtacgttcg cggtcgaact tcagcacttt aacagtgatt tcgtcgccca cgttgacgat
    18121 ttcgctcgga tgcttaacgc gtttccaggc catgtcagtg atgtgcagca ggccgtcaac
    18181 gccgcccaga tcaacgaatg caccgtagtc agtgaggttc ttaacgatac ctttaacttc
    18241 catgccttcc tgcaggtttt ccagcagctg atcgcgctct gcgctgtttt cggattcgat
    18301 aacggcacga cgagaaacaa caacgttgtt gcgcttctga tccagcttga ttactttgaa
    18361 ttcaagctct ttgccttcca ggtgcagagt gtcacgcacc ggacgaacgt ctaccagaga
    18421 acctggcagg aacgcacgaa taccgttcag ctcaacagtg aagccgccct taactttgcc
    18481 gttgataaca ccggtaacag tttcagcatc ttcgtaagct ttttccagcg tgatccaggc
    18541 ttcgtgacgt ttagctttct cacgggacag cagagtttca ccgaagccgt cttctactgc
    18601 gtccagagca acgtcaactt cgtcacctac ctggatttcc agctcgccct gggcgttttt
    18661 gaactgctca gccgggatgg cggactcaga tttcagacca gcgtcaacca gtactacgtc
    18721 tttgtcgata gcaacaacaa cgccacgaac gatagaaccc gggcgggttt cgatttcttt
    18781 taaggactct tcaaagagtt gagcaaaaga ttcagtcatg tttaatcttc aggtttatat
    18841 ttaacgtcca cctggctcca tgccggatgg ggttgtttaa catacccgct gtcattccat
    18901 tgcaacgggg gtactgcaaa ttcggtcgct tatgcgagag ccaatttctg gcgcgcgtat
    18961 tgtagcgctt tttcaatcac ttgctcaatg cttaaggtgg tggaatccaa cactaaagca
    19021 tcggctgccg gaaccagtgg cgctaccgct cggttacgat cgcggtcgtc gcgttctttg
    19081 atctcagcca aaaggcgctc aaagttaaca ctaaagccct tctcctgcaa ctgtagcatg
    19141 cggcgatgtg cacgttcttc cgaggaggcg tcaaggaaaa ttttcactgg cgcatcaggg
    19201 aataccaccg ttcccatgtc acggccatcg gcaatcaggc ctggtaattc gcgaaacgcg
    19261 cgttggcgac gcaataatgc ttcacgaacg cgtgggaatg ccgcgacttg tgaagctgca
    19321 ttcgccactt cctgagtacg aatttcgcca ctgacatctt ccccttcgag gatcacttcc
    19381 agattgccat tggtcgacac aaaacgtaca tccagatggg atgccagcgg caccagcgca
    19441 tcttccgatg caacatcaac atggtgatgt aatgccgcca gtgccagtac gcgataaatt
    19501 gcacccgagt ccagcagatg ccattgcaac gcttccgcca tagccttaca caaggtgcct
    19561 ttccctgcac cgcttgggcc atcaatggta ataaccgggg caattgccgt catctttatc
    19621 tccttaaaac aggcgtaccg ttaacataac cgcgcgcatt atacgcgccg acgccacaaa
    19681 tcgttaacgt tgtgtgcaaa aaccagtttt gtctgtattc agatggggat ttattgcgca
    19741 attatagcgg gctggcagaa gaccagcccg aaagatgaca atgatttact taccagaggc
    19801 gatacgatca cggatgtgtt gcgcacgctc tgttgatggt gggtgagaat caaacatgga
    19861 ctgggtacga ccgccatcca ggctggccaa tttttcaaag ctgccaacca gcccctgggt
    19921 gctgatgcca cgtttcttca acagatcaaa ggagaaatca tctgcttcgg actctttatc
    19981 gcgggagtat ttagcattga tcgcgccttc tgcgatatca cctaattgtg agcgagaaag
    20041 ctgggaagcc acaccgctgg tagcggaaat cgcatcacgt gcggcaacga tcgcataaga
    20101 agctttcatt tcagccagcg agtgacccaa cgcgacgtgg cccagttcat gacccagaac
    20161 gccttcaatt tcgttgtcgt tcatcatgtc catcaggcca ctgtagacac gaacacaacc
    20221 gttcgccatc gcccatgcgt tgacgtcgct ggtcatataa accttatagt tgaccggcgt
    20281 gccattaata ttgttaccca atgctttggc gatttttgcc agacgtttcc cgtacttgct
    20341 ggatttgctt gcgacttgat tgccgctgtc catttcggca cagccctgat tggcaatcgc
    20401 ttttgcatca gcatcgctta acgttgctgc tttataggca tttaaacctg agctgatagc
    20461 catattggta tcaataccgt gggtattttg acaccctgtc agtaatgctg cagaggccgc
    20521 aatcgccagc agtaatttag tattcttcat gtgaatcatc cgttgattaa tcatatataa
    20581 atattatgta tcaataactt acattgacaa gcgacgctac cattaatcaa aatgactcac
    20641 aaggtccgaa aaaaaacgcc ggcaatgtgc cgacgttttg ataaagaaag atttggctat
    20701 ttattgcacg ttgttcattc aggctgcctg gctaatccgc gccagctgct cgaaataatt
    20761 cggaaatgtt ttggccgtgc atttgggatc aagaatcgtc actggtgtat ctgacaacgc
    20821 caccagcgag aaacacatcg ctatccggtg atcattgtat gtcgcgatct cggcaaagtt
    20881 cagcttttcc ggtggagtga tacgaatgta atcgtgcccc tcttctactt ccgcaccaac
    20941 tttacgcagt tctgttgcca tcgcaaacag gcgatcggtc tctttaacac gccagttata
    21001 gatattgcgc agcgtggtgg tgccttttgc aaataacgcc gccgtggcaa tggtcatcgc
    21061 cgcatcggga atatggttca tatccatatc aatagcgttc agttcacccc gcgtgcagga
    21121 aatataatca tcgccccagc aaatggtcgc gcccattttt tccagcacat cagcaaagcg
    21181 aatatcaccc tgcatactgt tgcgtccaat gccggtcact tttacagtgc cgcctttgat
    21241 tgctgctgct gccaggaagt aagaagccga agatgcatcg ccttcgacca aataagtacc
    21301 cggagactga taagactgcc cgccttttac gacaaattgt tgatagtgct gattttcaat
    21361 ttcaacacca aacgtcttca tcatattgag tgtgatgtcg atataaggtt tagaaaccag
    21421 atcgccttta atacgaatca ccgtatcttc cggtgcaaga ggcgcagtca ttaatagtgc
    21481 ggtgaggaac tggctggaaa cggagccatc aacgtcaacg ttgccgccag taaagccgcc
    21541 ctgtaaacgc aatggcggat aattgtcttg ttccaggtaa gtgatcttcg ccccgcccag
    21601 gcgcagcgca tccaccagat gaccaatcgg gcgctctttc atacgcggct caccagtcag
    21661 cacaatatca ttgctaccca gacaaagagc tgccgccagc gggcgcattg ccgttccggc
    21721 gtttccgagg aacaactcca gggcaccttc tgcgtgtaat ggaccgccgt taccgataat
    21781 ttcgcaacgc gtacgatcgg ctgaaagcgt atagcttacc cctaacgctg ttaatgcatt
    21841 cagcatatgg cgcacgtcat cgctatccag cagattggtt aatactgttt tgccgtgtgc
    21901 taatgccgcc agcaataaag cgcggttaga aacgctcttg gaaccaggca gattaatagt
    21961 gccatcgaca cgagcgatgg gttgtaacgt cagggattcc atgaactcaa ctctctacaa
    22021 cagaaataaa aaccccacag gctggctgtg gggattaagc aaaatttcgg cattagccgt
    22081 gacggcgttc gaactcaacc atgaagtctg tcagggcttt aacgccttcc agcggcatgg
    22141 cgttataaat agacgcgcgc attccgccga ccacacggtg acctttcagc gcatgaaggc
    22201 cagcagcaaa agactcttca aggaacaatt tgtcaagcgc actgtccgcc aactggaacg
    22261 gcacgttcat ccgcgaacgg ttagctttcg ccacgtcatt gcggtagaaa ttgctgttat
    22321 caatcacccc atacagcagt tctgcttttt gctgattgat tttatccatt gcagctacac
    22381 cgccgttcgc tttcagccat ttaaagacca gaccagacag ataccaggca aatgtcggcg
    22441 gcgtgttaaa catggagtcg ttatcgttga gaatggaata atccagaatc gacggacacg
    22501 cgatattcgc tttgcccagc aaatcttcac gaacgatgac gattgtcagg ccagccgggc
    22561 cgatattttt ctgcgcgcca gcgtaaatca cgccataacg gctgacgtca atcggacggg
    22621 aaagaatggt tgaagagaag tcggcggcga ccaccacatc tttgccgaag tctggcgttt
    22681 cgtcgatagc gataccgtcg atggtttcat tcgggcagta atgcatataa gcagcattat
    22741 cagagagttg ccattcgcgc attggcttaa ccgcgcgcag accatcaaca gtcactttgg
    22801 cgtcaaagac attaggcgtg cagtattttt tcgcttcttt aatggcactt gccgcccagt
    22861 aaccggcatc aacataatct gcggtggttt tatcaccgag aatattcagc ggaacagcag
    22921 caaactgacc gcgaccgccg ccatggcaga acaatacctt gtagttggag gggacattaa
    22981 gaagatcgcg aaaatccttc tcggcttcct ctgcaacctg aatgaactct ttgccacggt
    23041 gactcacttc catcaccgac gtaccaagac cgttccagtc gcgcagttcc tgttgagcct
    23101 gtttaagcac ctctgccggt agcattgccg gaccagaact aaaattgaag atttgagcca
    23161 tttcccctca ccacgttgcg ttgctattaa cccgcggtca atcgattgcg accacaggta
    23221 atacattatt cctgtggcta tcggttttat cattcagtga cacacatcgc aatgagtaaa
    23281 accgttgagg gcaaaaatgt ggcttgcatc acacaaaaga atctctcgga ttattagcaa
    23341 aaagccgatg tttttactgt cacgtcaccc gttttgcccg acttgccttc gtccattctg
    23401 gatttgcaca gcgcggacat aattgttttt cccccgcgtt catatgaatg atttcactgc
    23461 aacgaatgca ggcatagtcc accgactcgg gtaccacttt gtaacgctga gtacagtcac
    23521 tgggtaaaat aagtaagccc ggtttcacct tgtcatcttc aaagaaatag acactgattt
    23581 gcccgttggt ttcgagaatc gccagacgta cttgccccaa ctgctctaca ccgcgcaatc
    23641 gtagctccat aaagaactca aattccgtca tgttggagtt attgagtttc gaccaggcca
    23701 gctcgccatc ttcaataatg acaaccggct tgccttccag aagatcttcc agtttttcac
    23761 tgtgcgccat caaccacatt accaggcggt ataacaacgc cagaataata aagacgataa
    23821 gtaccgggac catcggcaca tcatcataaa acgccacatc tcccgccgct gatcccagcg
    23881 tcagaatgat taacacttca aaaagcgaca tctgccgcac accgcgtctt ccggtcattt
    23941 tgaggaacaa aaaaaccagt acaaaggtat aaagactacg tagtgccact tcgccaagga
    24001 aatcaaaagg cactttatca aatgccatac ggtggagatc gaacgccttc atttatatgc
    24061 tcttatcgtg gtttatccgc taaaagcata gtcaacagat catcggcatg gcggcggtga
    24121 tagcgccttt gagataaaac ccgtattatt gcgcgctttc cgtacgacta aagtgatttt
    24181 catgacgcaa acatttatcc ccggcaaaga tgccgcactg gaagattcca tcgctcgctt
    24241 ccagcaaaaa ctttcagacc tcggctttca gattgaagag gcctcctggc tgaatccggt
    24301 gcctaacgtc tggtctgtac atattcgcga caaagagtgc gcactgtgtt ttaccaacgg
    24361 taaaggcgca accaaaaaag cggcgctggc ttctgcactc ggtgaatatt tcgagcgtct
    24421 ctcaaccaac tacttttttg ctgatttctg gctgggcgaa accatcgcca acggtccgtt
    24481 cgtgcattat cccaacgaaa aatggttccc actgagcgaa aatgacgatg tgccagaagg
    24541 gctgctcgat gaccgtctgc gcgcatttta cgatccggag aatgaactgg ccggcagtat
    24601 gctgattgac ctgcaatccg gtaacgaaga tcgtggtatt tgcggtctgc cgtttacgcg
    24661 tcagtctgat aatcagaccg tttatattcc gatgaatatc attggtaacc tgtatgtttc
    24721 taacggtatg tccgctggca atacccgtaa cgaagcacgc gttcaggggt tgtccgaagt
    24781 tttcgaacgc tacgtgaaaa accgcattat tgctgaaagc atcagcttgc cggaaatccc
    24841 ggcagacgtg ctggcgcgtt acccagcggt ggttgaagcc atcgaaacgc tggaagcaga
    24901 aggtttccca atctttgctt atgatggttc gcttggcggc cagtatccgg tgatttgcgt
    24961 ggtactgttc aatcctgcta acggtacctg ctttgcctct ttcggtgcgc atcctgattt
    25021 tggcgtagca ctggaacgta ccgtgaccga gctgctgcaa ggtcgtggcc tgaaagattt
    25081 ggatgtgttt actccgccaa ccttcgatga tgaagaagtt gctgaacata ccaacctcga
    25141 aacgcacttt atcgattcca gcggtttaat ctcctgggac ctgttcaagc aggatgccga
    25201 ttatccgttt gtggactgga atttctccgg cactacggaa gaagagtttg ctacgctgat
    25261 ggctatcttc aacaaagaag ataaagaagt ttatattgcc gattacgagc atctgggcgt
    25321 ttatgcttgc cgaattatcg tgcctggcat gtccgatatt tatccggctg aagatctgtg
    25381 gctggcgaat aacagtatgg gcagccattt acgtgaaacg attctttcgt taccaggcag
    25441 cgagtgggaa aaagaagatt acctgaacct catcgagcaa ctggatgaag aaggttttga
    25501 tgactttacc cgcgtgcgtg agctgttggg tctggcgacc gggtcggata acggttggta
    25561 caccctgcgt atcggtgaat taaaagccat gctggcgctg gctggtggcg atctggaaca
    25621 ggctctggtc tggaccgaat ggacgatgga gtttaactca tcggtattca gcccggaacg
    25681 cgccaactat tatcgctgcc tgcaaacgtt gttattactg gcgcaggaag aagatcgcca
    25741 gccgctgcaa tatctgaatg cgtttgttcg catgtacggc gcagatgccg tggaagccgc
    25801 cagtgcggca atgagcggcg aagcggcgtt ttatggtttg caaccagtag atagcgatct
    25861 gcacgcgttt gctgcacatc agtcgttgtt gaaggcctac gaaaagctgc agcgcgccaa
    25921 agcagcattc tgggcaaaat aaaatcaaat agcctacgca atgtaggctt aatgattcgt
    25981 ctgagttata ttacggggcg ttttttta
//
LOCUS       DAZKGF010000061        25926 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZKGF010000061 DAZKGF010000000
VERSION     DAZKGF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.061"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(213..1862)
                     /gene="pgi"
                     /locus_tag="ACHOQV_002353"
     CDS             complement(213..1862)
                     /gene="pgi"
                     /locus_tag="ACHOQV_002353"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP9630720.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2099..2295
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2387..3736
                     /gene="lysC"
                     /locus_tag="ACHOQV_002354"
     CDS             2387..3736
                     /gene="lysC"
                     /locus_tag="ACHOQV_002354"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFP9630721.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3989..4261
                     /locus_tag="ACHOQV_002355"
     CDS             3989..4261
                     /locus_tag="ACHOQV_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFP9630722.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4262..5134)
                     /gene="rluF"
                     /locus_tag="ACHOQV_002356"
     CDS             complement(4262..5134)
                     /gene="rluF"
                     /locus_tag="ACHOQV_002356"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFP9630723.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5482..6429
                     /locus_tag="ACHOQV_002357"
     CDS             5482..6429
                     /locus_tag="ACHOQV_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFP9630724.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVNALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6499..7305
                     /locus_tag="ACHOQV_002358"
     CDS             6499..7305
                     /locus_tag="ACHOQV_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9630725.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTRLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVTDFVCYLLSQRASYITGVTTNIAGGKTRG"
     gene            7315..7722
                     /locus_tag="ACHOQV_002359"
     CDS             7315..7722
                     /locus_tag="ACHOQV_002359"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFP9630726.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     HSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            7722..8216
                     /locus_tag="ACHOQV_002360"
     CDS             7722..8216
                     /locus_tag="ACHOQV_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9630727.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQHIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8282..9079
                     /locus_tag="ACHOQV_002361"
     CDS             8282..9079
                     /locus_tag="ACHOQV_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP9630728.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9090..9914
                     /locus_tag="ACHOQV_002362"
     CDS             9090..9914
                     /locus_tag="ACHOQV_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9630729.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLLVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9966..11195
                     /gene="sorE"
                     /locus_tag="ACHOQV_002363"
     CDS             9966..11195
                     /gene="sorE"
                     /locus_tag="ACHOQV_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFP9630730.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAIGGGKKLVYTGKYLPLMSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            11577..12266
                     /gene="pepE"
                     /locus_tag="ACHOQV_002364"
     CDS             11577..12266
                     /gene="pepE"
                     /locus_tag="ACHOQV_002364"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFP9630731.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12356..13987)
                     /locus_tag="ACHOQV_002365"
     CDS             complement(12356..13987)
                     /locus_tag="ACHOQV_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFP9630732.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14207..17890)
                     /gene="metH"
                     /locus_tag="ACHOQV_002366"
     CDS             complement(14207..17890)
                     /gene="metH"
                     /locus_tag="ACHOQV_002366"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP9630733.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMEFLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLSGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18090..18914
                     /gene="iclR"
                     /locus_tag="ACHOQV_002367"
     CDS             18090..18914
                     /gene="iclR"
                     /locus_tag="ACHOQV_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFP9630734.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            complement(18931..20655)
                     /gene="aceK"
                     /locus_tag="ACHOQV_002368"
     CDS             complement(18931..20655)
                     /gene="aceK"
                     /locus_tag="ACHOQV_002368"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFP9630735.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGNLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRK
                     QRFSVRFV"
     gene            complement(20871..22175)
                     /gene="aceA"
                     /locus_tag="ACHOQV_002369"
     CDS             complement(20871..22175)
                     /gene="aceA"
                     /locus_tag="ACHOQV_002369"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFP9630736.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(22205..23806)
                     /gene="aceB"
                     /locus_tag="ACHOQV_002370"
     CDS             complement(22205..23806)
                     /gene="aceB"
                     /locus_tag="ACHOQV_002370"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFP9630737.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKHILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(24075..25004)
                     /gene="metA"
                     /locus_tag="ACHOQV_002371"
     CDS             complement(24075..25004)
                     /gene="metA"
                     /locus_tag="ACHOQV_002371"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFP9630738.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            25161..25604
                     /locus_tag="ACHOQV_002372"
     CDS             25161..25604
                     /locus_tag="ACHOQV_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9630739.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAAYRAELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNKQAVGFYKKMGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            complement(25730..25845)
                     /gene="rrf"
                     /locus_tag="ACHOQV_002373"
     rRNA            complement(25730..25845)
                     /gene="rrf"
                     /locus_tag="ACHOQV_002373"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6116 a         6774 c         6788 g         6248 t
ORIGIN      
        1 acagacaagg ggatttatct gataaaaaat gccgacataa cgtcggcatt gtttttaagg
       61 ctatttaatc aatcgtacta tgtattaatt gccgaatacg atgtacatat cggcatcgac
      121 ctgtaggcct gataagacgc gaccgcgtcg catcaggcat cggttgccgg atgcggcgtg
      181 aacgccttat ccggcctaca tatcgacaat gattaaccgc gccacgcttt atagcggtta
      241 atcagaccat tggtcgagct atcgtggctg ctgatttctt tatcatcttt cagctctggc
      301 agaatacggt tcgccagctg tttacccagc tccacgcccc actggtcgaa ggtgaagatg
      361 ttcagaatca cgccctgagt aaagattttg tgctcataca gcgcaatcaa tgcacccagg
      421 ctgaacggag tgatttcacg cagcaggatg gagttggtcg ggcggttacc ttcgaacact
      481 ttgaacggca ccacgtagtc aagcgttgcc ggatctttac cctgatcacg atattcctgc
      541 tcaaccactt cgcgggattt accaaacgcc agcgcttcgg tctgagcaaa gaagttagac
      601 agcagtttct ggtgatgatc agagagcggg ttatgggtga tagccggagc gatgaaatcg
      661 cacggtacca ttttggttcc ctggtggatc agctgataga acgcgtgctg accgttcgtg
      721 ccaggttcac cccagataat cgggccagtc tggtaatcca caaccttacc gttacggtca
      781 acatacttac cgttggattc catattgccc tgctggaagt acgccgcgaa acggtgcata
      841 tactggtcat acggcagaat cgcttcagtt tccgcgccaa agaaattgtt gtaccagatg
      901 ccaatcagcg ccagcaagac aggcaggttt ttctcggcag gcgtggtgga gaaatgcttg
      961 tccatcgcgt gtgcgccaga aagcagttca acaaaattat caaagccgat ggagagaaca
     1021 atcgacaggc caattgctga ccacaaagag taacggccgc caacccagtc ccagaactcg
     1081 aacatgttgg cagtatcaat accaaactcg ccaacggctt tggcattggt ggaaagcgcc
     1141 gcaaagtgtt tcgcaacgtg tttttcatca ccagccgctt tcaggaacca gtcacgcgcg
     1201 ctgtgggcgt tggtcatggt ttcctgagtg gtgaaggttt tagatgctac caggaacagc
     1261 gtggtttccg ggtttacttt tttcagcact tccgcgatgt gagtcccatc gacgttagaa
     1321 acaaagtgca tgttcaggtg gtttttgtat ggacgcagtg cttcagtcac catgtacggg
     1381 ccgaggtcag aaccgccgat cccgatgttc actacgtcag tgattgcttt gccggtatag
     1441 cctttccact caccggaaat aatcgcttcc gagaaggttt tcatcttctc cagcaccgcg
     1501 ttgacttctg gcattacgtc tttgccatca accagaatcg gggtattgct acggttacgc
     1561 agcgctacgt gcagcacggc gcggttttca gtgcggttga tcttctcgcc agagaacatc
     1621 gacttaatcg cgcccgccag atcgcactct ttcgccagat cttgtaattt cgccagcgtc
     1681 tcttcagtga tgcggttttt ggagtaatcc accagcatct gatcgtcgaa tgttgcggag
     1741 aacttagaaa aacgatcgcc gtctttagca aaaagatcgg cgatcgtaac gtctttcatt
     1801 tcatcgaagt gtttctgtag tgcctgccag gcagcggtct gcgttggatt gatgtttttc
     1861 attagcaata ctcttctgat tttgagaatt gtgactttgg aagattgtag cgccagtcac
     1921 agaaaaatgt gatggtttta gtgccgttag cgtaatgttg agtgtaaacc cttagcgcag
     1981 cgaagcattt attagctgaa ctactgaccg ccaggagtgg atgaaaaatc cgcatgaccc
     2041 catcgttgac aacggccccg ctcacccttt atttataaat gtactacctg cgctagcgca
     2101 ggccagaaga ggcgcgttgc ccaagtaacg gtgttggagg agccagtcct gtgataacac
     2161 ctgagggggt gcatcgccga ggtgattgaa cggctggcca cgttcatcat cggctacagg
     2221 ggctgaatcc cctgggttgt caccagaagc gttcgcagtc gggcgtttcg caagtggtgg
     2281 agcacttctg ggtgaaaata gtagcgaagt atcgctctgc gcccacccgt cttccgctct
     2341 tcccttgtgc caaggctgaa aatggatccc ctgacacgag gtagttatgt ctgaaattgt
     2401 tgtctccaaa tttggcggta ccagcgtagc tgattttgac gccatgaacc gcagcgctga
     2461 tattgtgctt tctgatgcca acgtgcgttt agttgtcctc tcggcttctg ctggtatcac
     2521 taatctgctg gtcgctttag ctgaaggact ggaacctggc gagcgattcg aaaaactcga
     2581 cgctattcgc aacatccagt ttgccattct ggaacgtctg cgttacccga acgttatccg
     2641 tgaagagatt gaacgtctgc tggagaacat tactgttctg gcagaagcgg cggcgctggc
     2701 aacgtctccg gcgctgacag atgaactggt cagccacggc gagctgatgt cgaccctgct
     2761 gtttgtcgag atcctgcgcg aacgcgatgt tcaggcacag tggtttgatg tacgtaaagt
     2821 gatgcgtacc aacgaccgat ttggtcgtgc agagccagat gtagccgcgc tggcggaact
     2881 ggccgcgctg cagctgctcc cacgcctcaa tgaaggctta gtgatcaccc agggatttat
     2941 cggtagcgaa aataaaggtc gtacaacgac gcttggccgt ggaggcagcg attatacggc
     3001 agccttgctg gcggaggctt tacacgcatc tcgtgttgat atctggaccg acgtcccggg
     3061 catctacacc accgatccac gcgtggtttc cgcagcaaaa cgcattgatg aaatcgcgtt
     3121 tgccgaagcg gcagagatgg caacttttgg tgcaaaagta ctgcatccgg caacgttgct
     3181 acccgcagta cgcagcgata tcccagtctt tgtcggctcc agcaaagacc cacgcgcagg
     3241 tggtacgctg gtgtgcaata aaactgaaaa tccgccgctg ttccgcgcgc tggcgcttcg
     3301 tcgcaatcag actctgctca ctttgcacag cctgaatatg ctgcattctc gcggtttcct
     3361 cgcggaagtt ttcggcatcc tcgcgcggca taatatttcg gtagacttaa tcaccacgtc
     3421 agaagtgagt gtggcattaa cccttgatac caccggttca acctccactg gcgatacgtt
     3481 gctgacgcaa tccctgctga tggagctttc cgcactgtgc cgggtggaag tggaagaggg
     3541 tctggcactg gtcgcgttga ttggcaatga cctgtcaaaa gcctgcggcg ttggcaaaga
     3601 ggtattcggc gtactggaac cgttcaacat tcgcatgatt tgttacggcg catccagcca
     3661 taacctgtgc ttcctggtgc ccggcgaaga tgccgagcag gtagtgcaga agctgcattt
     3721 taatttgttt gagtaaacac agtatggccc ggaagacatt atttcgggcc gtattgtttt
     3781 tcttgtcatt atgcccataa ataaacgagc ctgtactctg ttaaccagcg tctttatcgg
     3841 agaataattg cctttaattt tttatctctt tctctaacta attagagaaa gagataaata
     3901 gttaagagaa ggcaaaatga atattatcag ttctgctcgc aaacgaattc ccgtacactg
     3961 aactctctgg ctcactgagg gcaccatcat ggcactcccc cgtattaccc aaaaagagat
     4021 gactgaacgc gagcagcgtg aacttaaaac actgctggat cgggcgcgga ttgcgcatgg
     4081 tcgcgtactg accaactccg aaaccaacag cattaagaaa gagtacatcg ataaattaat
     4141 ggtcgagcgt gaggctgaag cgaaaaaagc ccgccaattg aagaaaaagc aggcttataa
     4201 accggatcct gaggcgtcat ttagctggtc agcaaatacg tcgacgcgcg gcaggcgctg
     4261 attaacgccc cttctttttg cgccccggcg aggtaaaacg cttaccgttg gacgccgggc
     4321 gaccgccttt ttccgccgtt ttttccatct taacgactgg acgtttgatg cctaccgttt
     4381 tcggtttggc cttcgcttta ggttttgcct cggaagagga attttcgatg agcttaaaga
     4441 ggtcgattaa ctcatcgtcg gttaaatcgc gccactcccc cagcggaatg ccgcttaaac
     4501 tgacgttcat aatgcgcgta cgttccagct ttttcacttc gtaaccaaag tgctcgcaca
     4561 tgcgacggat ctgacggttc agcccctgca ccagggtaat gcggaagaca aatggcgctt
     4621 cttttttgac tttgcacttt ttagtcactg tcccgaggat tggcaccccc gcccccatac
     4681 cacgaataaa ttcatcggta atcggtttat cgaccgtcac cagatactct ttctcatgat
     4741 cgttacccgc acggaggatc ttattcacca gatcgccgtg attggtgagg aaaatcagcc
     4801 cctgggagtc tttatccagg cggccaatcg ggaatacgcg tttgctgtgg ttaacgaaat
     4861 cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac aataccgacg ggcttgttca
     4921 gggcgataag taccaaatct tcggcttccc gaggttcaat caactgacca tttactttca
     4981 caacgtcgcc gggtttcacc tgatcgccaa tggtggctcg cttgccatta aggaacacat
     5041 tgccttgctc gatatagcga tccgcttcgc ggcgtgagca aattccgctt tcgctgatgt
     5101 atttatttaa acggactgat gagtcgggca gcataaattc tcctgtaaaa cgcggaatat
     5161 accgtacctc ttagcgggga aaaacagggt tatgcggcaa cgtcgaaatt aaatgtgatc
     5221 tgccacgcct tttatcacaa aagtgtctcc ggaagtacga tgcaaatttg cctggcaaaa
     5281 taaggataat caacatatta cgaatcgctg cacacctgtg ctgatgacta ctacgcagag
     5341 aaacaaatgt gcaagagaaa gcgctatttc tccccatgca aagagtgaaa aatcgctttg
     5401 aaaggccctc atgatggggt tatatcagat caagcggtga gaaagcgtat ttgcacaaat
     5461 gtgcagaacc aggaggcggc aatggaaaac agtgacgata tccgtttgat tgtgaagatt
     5521 gcccaactct attacgaaca ggatatgacg caggcgcaaa tcgcgcgcga actgggtatt
     5581 taccgcacca ccatcagccg cttgcttaaa cgaggccgcg atcagggaat tgtcaccatc
     5641 gccatcaact atgactacaa cgaaaatctc tggctggagc agcaactgaa gcaaaagttt
     5701 ggcctgaaag acgttgtggt ggtgtcagga aatgatgagg atgaagagac tcaactggcg
     5761 atgatggggt tacacggcgc gcaactgctg gatcgcttgc tggaacctgg cgatattgtc
     5821 ggtttttcct ggggccgcgc ggtgaacgca ctggttgaaa acttgccgca ggcggggcaa
     5881 tcgcggcagt taatctgcgt accaattatt ggcggaccgt ccggtaaact cgaaagccgc
     5941 tatcatgtaa acacattaac ctacagcgcg gcagcgaagt tgaaagggga atcgcatctc
     6001 gcggattttc cggctctgct ggataaccca ttaattcgta atgggatcat gcagtctcag
     6061 cactttaaaa ccatctctgc ctactgggat aatctggatg tcgccctggt gggaattggc
     6121 tcaccggcca ttcgcgacgg cgctaactgg catgcgtttt atggtggtga agagagtgac
     6181 gacctgaatg cccgccaggt tgctggcgat atttgctcgc gcttttttga tattcacggc
     6241 gcaatggttg aaacaaatat gagcgaaaaa acactctcta tcgaaatgaa taaattaaag
     6301 caggcgcggt attccattgg cattgccatg agtgaagaaa aatacagcgg aattattggt
     6361 gcactgcgtg gaaaatatat taattgtctg gtaacgaata gcagcacagc tgaactatta
     6421 ctgaaataaa aatcttcccg atgtttaccg taataaaaat tacggcgggt ctctattatc
     6481 taaaatcagg agtctgttat gcaaacgtgg ttaaatttgc aggataaaat cattattgtc
     6541 accggtggcg catccggcat tggtctggcg attgtggatg aattattagc acaaggcgcg
     6601 aatgtacaga tggtcgatat tcacggtggc gatggacaat atgaaggtca taaaggctat
     6661 cagttttggc cgaccgatat ttccagcgcc aaagaggtaa atcatacggt agcggaaatt
     6721 atccagcgtt ttggtcgcat cgacggtctg gtcaataacg ccggggtcaa tttcccgcgt
     6781 ctgctggtcg atgagaaagc gcctgccggg cagtatgaac tcaacgaagc tgcattcgaa
     6841 aaaatggtca atatcaacca gaaaggcgtt tttctgatgt cgcaggcggt ggcgcgacag
     6901 atggtcaaac aacatgatgg cgtgattgtg aatgtttcct cagaaagtgg gctggaaggc
     6961 tcagaaggcc aaagctgtta cgccgctacc aaagccgcgc ttaatagctt cacgcgatcc
     7021 tggtcgaaag aattgggtaa acacggtatc cgtgtagtcg gtatcgcgcc ggggattctg
     7081 gaaaaaacca ggctacgcac gccggaatat gaagaggcgc tggcgtggac gcgcaatatc
     7141 accgtcgagc agctgcgtga aggctatacc aaaaacgcca ttcctattgg gcgcgccgga
     7201 agattagcag aagtcactga ttttgtttgt tatctgctgt ctcaacgcgc cagctatatc
     7261 accggagtaa ccactaacat tgcgggcggc aaaacgcgcg gctaaggagg ctttatggtc
     7321 aacgccattt tttgtgccca cggcaaatta gcttgcgcca tgctggaatc ggttcagatg
     7381 gtttacggca acgccaatgt tgaagccgtt gaatttgtgc ccggtgaaaa cgccggagac
     7441 atcgtagcaa agctggaaaa gttagtaagc attcacaatc aagatgagtg gctgattgcc
     7501 gtggatttac agtgcggcag cccgtggaat gcggcagcga tgcttgcgat gcgcaatcct
     7561 cgtctgcggg taatcagcgg cctttctctg ccgctggcgc tcgaactggt tgataaccag
     7621 cacagcatga atgttgatga actgtgtgaa cacctgacgc aaatcgcaaa gcaaacctgc
     7681 gttgtctgga aacaactggc aacggcagag gaggatttct gatgaacatt acgctcgccc
     7741 gcattgatga ccgtctgatc cacggccagg tcactaccgt ctggtcgaaa gtggctaatg
     7801 cccagcacat tattatctgc aatgacgaag tttataacga tgaagtgcgc cggacattat
     7861 tgcgtcaggc tgcgccaccg ggaatgaagg ttaacgtggt caatattgaa aaagccgtcg
     7921 cggtttatca caacccgcaa tatcaggacg aaaccgtttt ttatcttttc actcgcccac
     7981 aggatgcctt agcgatggtt cgccaggggg taaaaataga caccctgaat attggcggca
     8041 tggcctggcg gcccggtaaa aaacagttaa ccaaagcggt ttcattagat gatgatgata
     8101 ttaacgcatt tcatgagtta aataatcttg gcgtcatact ggatttacgc gtcgtagcgt
     8161 cagatccctc aatcaatatt atcgacaaaa taaacgaaca gttaatcgca aattaaataa
     8221 tagcgcctgt gtacatatgc tttaacaggc agggatgacg acacttaaaa ggtgacaaat
     8281 catggaaata agtaccctac aaatcattgc tatatttctt ttttcctgta ttgccggaat
     8341 gggcagcgtg ctggatgaat ttcagactca tcgtccgtta attgcctgta cggtgattgg
     8401 tttaattctc ggtgatttaa aaaccggaat tatgctcggt ggtacgctgg aattgatagc
     8461 tctcggctgg atgaacgtcg gcgcggcgca atctccggat tctgcactcg ccagcataat
     8521 ctccgccatt ctggttatcg ttggtcagca aagcatcgcc accggaatcg ccatcgcgtt
     8581 gcctgtggct gcggcaggcc aggtgctgac ggtgtttgcc cgtaccatca ccgtagtgtt
     8641 ccagcacgcg gcggataaag cagcggaaga agcacggttt cgcactctcg atattctgca
     8701 tgtctccgcg cttggcgtgc aggcactgcg cgttgctatt ccggcactga ttgtctcgct
     8761 gttcgtcagc gctgatatgg tgagcaatat gctgagcgcc attcccgaat ttgtgacccg
     8821 tggactgcag attgctggcg gttttatcgt ggtggtcggt tacgccatgg tgcttcgcat
     8881 gatgggcgtg aaatatttga tgcctttctt tttcctcggt ttcctcgcag gtggctacct
     8941 cgatctcagt ctgctggcgt tcggtggcgt cggcgtgatc atggccctgc tctacatcca
     9001 gctaaatcca cagtggcgta aagctgaacc tcaactccag gccaccgcct ccaccgccct
     9061 tgaccaactt gacgattaac ggagccatca tggaacagag aaaaattaca cgcagcgatc
     9121 tggtgagcat gtttctgcgc tccaacctgc aacaggcgtc ctttaacttt gaacgtattc
     9181 acgggctggg tttttgctac gacatgatcc ccgccatcaa gcgactttac ccattaaaag
     9241 aggatcaggt tgcggcgctc aggcgacacc tggtgttctt caataccacg ccagccgtat
     9301 gtggcccggt catcggcgtc actgccgcca tggaagaggc gcgggccaac ggcgcggaaa
     9361 ttgatgacgg taccattaac ggcatcaaag tcggtctgat gggaccattg gcaggagttg
     9421 gcgatccact ggtctgggga acgctgcgcc cgattaccgc cgcgctcggc gcatctctgg
     9481 cactttcggg caacattctc ggcccgctgc tgtttttctt tattttcaat gcggtgcgtc
     9541 tggcgatgaa gtggtatggc ctacagctcg gctttcgcaa aggggtgaat atcgtcagcg
     9601 atatgggcgg gaatgtgctg caaaaactca ccgaaggcgc gtcgattctc ggactgtttg
     9661 tgatgggcgt gctggtgacc aaatggacgt caatcaacgt accgttgctg gtttcacaaa
     9721 cgcatgccgc cgatggctcc accgtcacca tgaccgtgca gaacattctc gaccaacttt
     9781 gccctggttt gctggcgctc ggtctgacgc tgctgatggt tcgtctgctc aacaaaaaaa
     9841 ttaacccggt atggctgatt ttcgccctgt ttggtttagg gattatcggt aatgcgctgg
     9901 gcttcctgtc ctgattcttc gccccggcac gactgccggg gtcatcgctc aacatgaggt
     9961 ggtttatgaa aacaacagct ctgcgtcttt atggcaaacg tgacctgcgt ctggaaacct
    10021 ttgaccttcc tgaaatgcag gaggatgaaa tcctcgcaac ggtggtcact gacagcctgt
    10081 gcctctcttc ctggaaagag gccaatctgg gtgaaaacca taaaaaagta cccgacgatg
    10141 tggcgaccaa ccccatcatc atcggccacg agttttgcgg cgatattctg gccgtgggta
    10201 aaaagtggca gcacaaattc cagccgggtc agcgttatgt gattcaggcc aacctgcaac
    10261 tccccgaccg cccggattgc cccggctact ccttcccgtg ggtaggcggc gaagcgacgc
    10321 atgtggttat tcccaacgag gtcatggaac aagattgcct gctggcatac gacggcgaaa
    10381 cctattttga aggctcgctg gttgaaccgc tttcctgcgt gattggcgcg ttcaacgcca
    10441 actatcatct tcaggaaggt agttataacc acacgatggg gattcgcccg caagggcgca
    10501 cgctgatcct cggcggcacc ggcccgatgg ggctgctggc gattgattat gcgttgcatg
    10561 gaccggttaa cccgtcgctg ctcgtcatta ccgataccga caacgacaaa ttgagttatg
    10621 cacgcaagca ctatccatca gaaccgcaaa cactgattca ttatctcaat gccgccgatg
    10681 cagcatttga tacgttaatg gcactgagtg gcggtcacgg cttcgatgat attttcgtct
    10741 ttgtgcctaa tgaaggactg gtgactctcg cctcttcctt gctggcgaca gatggttgtc
    10801 tgaatttctt cgccggaccg caggataagc acttcagcgc gccaattaat ttctacgatg
    10861 tgcattatgc atttacccac tacgtgggca cgtcaggcgg caataccgac gacatgcgcg
    10921 cagcggtcaa attgattgaa gagaaaaaag tgcaggccgc aaaagtggta acacatattc
    10981 ttgggctgaa tgccgcgggc gaaaccacgc ttgaattgcc tgccatcggc ggcggcaaaa
    11041 agctggtgta taccgggaaa tacctgccgc ttatgtcact cacgcagatt caggatcaag
    11101 cactggcggc aattctggcg cgtcatcagg ggatctggtc cggtgaggcg gagcaatacc
    11161 tgctcgccca tgcagaggca atttcccatg attaatcgcg ctacttaact ctgcatgtcg
    11221 ctggctggtc gccgcggcaa cttcgcgacc tgatgtgcgg ctgattgctg aagcggcaca
    11281 atgtgcacga gaaggataaa aaaacatcag cgccttaagg ggcgctcaaa ttggtggcaa
    11341 agagcacatt gtttatgccg gatgcggaat gaagccttat tcggcctaca taatcttgaa
    11401 aattcaatat attgcagtta tctggtaggc caggtaagcg tagcgcatca ggcaatcctg
    11461 tgtttgtcat cagtctcagc gcctgtgcaa tatgtgacat cgtgagccat gtcagtaagt
    11521 ggttaaattc gcagtggtag cattccttcc gtgttttctt actaaccgga gaagtaatgg
    11581 aactgctttt attgagtaac tcgacgctgc cgggtaaagc ctggctggaa cacgcgctgc
    11641 cgctgattgc tgaacagttg cagggtcgcc gttctgcggt atttattcct ttcgcgggcg
    11701 taacgcagac ctgggatgat tacacagcga aaacggctgc ggttctcgct ccgctgggtg
    11761 tttctgtcac cggtattcat agcgttgtcg atcccgttgc cgcgattgaa aatgcggaaa
    11821 ttgtgattgt cggcggcggt aatactttcc agttgctgaa acagtgccgc gagcgcggac
    11881 tgctggcacc aattactgac gtggttaaac gtggcgcgct gtatattggc tggagcgctg
    11941 gcgctaacct tgcttgccca accattcgta ccaccaacga tatgccgatt gtcgatccac
    12001 aaggtttcga tgcgctgaat ctgttcccgt tgcaaatcaa cccgcacttc accaacgcgc
    12061 tgccggaagg ccataaaggt gaaacccgtg agcagcgtat tcgcgaactg ctggtcgtcg
    12121 cgccagaact cacaattatt ggtctaccgg aaggtaactg gatcacggtg agtaaaggtc
    12181 acgctacgct gggtggcccg aacaccactt atgtgtttaa ggctggtgaa gaagcggttc
    12241 cgctggaagc gggtcaccgt ttttaagtga attgccggat gcatgatccg gcatcttgta
    12301 catagtgcct gatgcgacgc tttacacgtc ttatcaggcc ttcagggtca cggtattaat
    12361 actcgtcccg tccttcgtct tcatccggct gttccagcac actgtaagcc accgaacaaa
    12421 acagcgagtt cagacgctgc atatctccca gtaagcctaa atgtagcgaa ctggtttcaa
    12481 tactctgcac gttttgctga tgcaggcgat cgacgtgagc atgggaataa cggcgattaa
    12541 gaatgcgaaa gcgatgcttg ctgcggcgca aacgacgggc gctggtaaca tcgccagaga
    12601 agaaaaccga cattgccagt tttaaattac tgagcaattg ctcatagagc gcatccagtt
    12661 ccttcaagcc atcaagcgaa aatgcccgcc gtgctgccag cgatttatcg gcaatttcac
    12721 tccccatgcg ctcgacgata tcggaggcct gttcaaggtt gagcgacatt tcgatgatct
    12781 ccgcccagcg gcgcgactct tcttctgcca gttcctcttt tggcatccgc gccagataca
    12841 gcttaatggc ggtatagaga acgttgatgt catcagccag cttacgcagc tctttctcct
    12901 gccgtggctc gccgtgcatc actttattca acccttccat catctgttcc atggcgtcgc
    12961 caatgcgcag ggtttcgcgg gcagcgttcg ccagagcaag cgtgggcgta tccagcgcgc
    13021 tgacatccag atgtttaggc cgtagctggg tatccagttc cggttcatcg cgaataatcg
    13081 ttttgcaaaa ccgtgccatc gggtcaacaa atggcagcat gaccaggcag cgtacaaggt
    13141 tgtagaagac gtggaaatag atcaccaatt ccgcttttgg caatggcaac ttccccattg
    13201 tctctgccag caaatgaaca aacggcagga taatcaggct acccaccagc ttaaacagca
    13261 ggctacccag cgcgacacgg cgggctgcgg cattggcggc actgttgttg agcatcgcca
    13321 gcaggccgga accgaggttt gcaccaatca ccagacaaag cgccactggg aaagagataa
    13381 tgcctgcggc ggtcagagtc gcggtcagca gtaccgcggc aaggctggag tagctgataa
    13441 tggcgaacat cgcaccaatc agcgcatcca gcagaatatc gccggtcagc gaggcaaaga
    13501 tcacctgaac gccgtttgcc tgggtgatcg gcgttacggc ctgcacaatc aactccagcg
    13561 ccagcaaaat caacccaaga ccaataccga cgcggcccag ttgcccggcg cgtgactgtt
    13621 tgcgtccgag gaaaaaaatc acgccgataa aaataagtaa tggtgacagc caggataagt
    13681 cgaaggtgag aatacgcgcc attagcgccg tcccgacatc tgcacccagc acaatcacca
    13741 gagccggtgc gagggctacc agatcctgag cgacgaacga ggtcaccagc atagtggtgg
    13801 cattactgct ctgcaccagt gcggtaacgc ctatccccgc gcaaaaggcg agcggctttt
    13861 tttcgacgct ccggctaagg acagtacgca aacgcgcgcc gaagacgcgc attacgccgg
    13921 ttcgaacaat atgagtcccc cagaccagca gggcgacggc agaaagcagg tgaagcagcg
    13981 ttaacacgag ttcaggttct ccttatcgtt atatgttcct gaagcaattg aatgaccaaa
    14041 ggaccggaaa cacgctgact ggcatcttta ctgtgccttt tgaccttcgt ttttccggct
    14101 aagattattc cagcctgttt ccagtataag tgtttcatat taataaagag acagggcact
    14161 cattgagcgc cctgtttgtt gtaaggaaaa gtgacagatt tgtgaatcag tccgcgtcat
    14221 accccagatt cggtgccagc cagcgctcaa catctgagac gctcatacct ttacggcggg
    14281 catagtcttc aacctgatcg cgctggattt gcgccaccgc gtaatacttg ctgtccgggt
    14341 ggctgaagta ccaacccgaa accgatgcac caggccacat ggcgaaagat tctgtgagtt
    14401 tcatgccagt gtgtttttcc acttccagca gctcccagat ggtggctttt tccgtatgtt
    14461 ccgggcaggc cggataaccc ggtgccggac ggatgccctg gtagttttcg cggatcagct
    14521 cttcgttgct gaggttctcg ttcggcgcat agccccagta gactttacgc acacgctcat
    14581 ggagatactc cgcaaaggct tcggctaaac ggtcggcaag cgctttcacc atgattttgt
    14641 tgtaatcatc gtgctgcgct tcaaaggcat cagccagtgc gtcctcttcc agcccgccag
    14701 tcacggcaaa tgcgccgatg taatctgctt taccagaaag cttcggcgca acgaagtcag
    14761 cgagacagta gttagcgaag ccggtttttt cggtctgttg acgcagatgg tggctgacgt
    14821 tgatcacatg ggtacgcgtt tcgtcacggt agatttcaat gtcatcgccc acacggtttg
    14881 ccgggaacag gcccaccacg ccacgcggat tcagcgtttt ttcggcgctt aatttatcca
    14941 gcatgtcgtt ggcgtcttta aacagccgct gcgcctcaac gcccaccact tcatcttcca
    15001 gaatgcgcgg atacttcccg gacagcgacc aggtcataaa gaacggtgtc cagtcgatgt
    15061 aattacgcag cgtttcgatg ctggcttcga cttcctgcac accgagacgg tgcgccaccg
    15121 gcggcgtgta agcctgccag tcaaaagcga agtcgttatc acgcgccgct tccagcgtga
    15181 ccggtggtgt gcgtggtttc tttcgtccgt gctgaatacg cacggtttcg tactccttgc
    15241 gggtacgagc aacgaaatca tcgcgctgag tgtcggaaag caacgccgcc accacaccaa
    15301 cggtgcgtga agcgttctgc acatacaccg tcgggccgct gtagttctgc tcaattttca
    15361 ccgccgtgtg cgcttttgag gtggtcgcgc cgccaatcaa tagcggaatg gtgaagccct
    15421 gacgctccat ctctttcgcc acgttaacca tctcgtccag cgacggcgtg atcagccccg
    15481 aaaggccaat caaatcagca ttcacttctt tagcggtacg gagaattttt tccgcaggca
    15541 ccatcacgcc gagatcgaca atttcgtagt tgttacattg cagcaccacg ccaacgatat
    15601 ttttaccgat gtcgtggaca tcgcccttca ctgtggcgat caccatcttg ccgttggttt
    15661 taccctgctc tttgctggct tcaatgaacg gctcgagata cgccaccgcc tgtttcatga
    15721 cgcgcgccga tttcaccacc tgcggcagga acattttgcc ctcgccaaac aggtcgccga
    15781 ccacgttcat gccgtccatc aacgggcctt caatcacttc aatcggacgc gtagcctgct
    15841 ggcgggcttc ttcggtatcc tgctcgataa actcggtaat gcctttgacc agcgagtatt
    15901 ccagacgttt attcacttcc cacgagcgcc actctgcctg ctgggtgtta gcggtgtcgt
    15961 cggttttgct gccgcgatat ttctcggcaa gctcgagtaa acgctcggtg ccatcgtcgc
    16021 gacggttgag gatcacatcc tccaccgcgt cgcgtagctc ggtaggcagg tcgtcgtaaa
    16081 tcgccagttg cccggcgttg acgatcccca tatccatgcc attgcgaata gcgtagtaga
    16141 ggaacaccgc gtgaatggct tcgcgcaccg gatcgttgcc acggaatgag aaagaaacgt
    16201 tagaaacgcc gccggaaatc agtgcgtgcg gcagttcacg tttgatgtct tcacacgcgc
    16261 cgataaagtc ctgcgcgtaa ttgttgtgct cttcaatgcc agttgcgacc gcgaagatgt
    16321 tcgggtcgaa gatgatatct tcagccggga agccaacctc ttcggtgagg attttgtacg
    16381 cccgacggca aatctcgatt ttccgtgcgc gggtatcggc ctggccctgt tcgtcaaaag
    16441 ccattaccac gactgccgca ccgtagcgac gcaacagttt cgcgtgatgg ataaaggcgt
    16501 cgacgccctc tttcatcgag atagagttaa caatgccttt gccctggata cacttcagac
    16561 ctttttcaat aacgtcccat tttgaggagt cgatcattat cggcacgcga gcgatatccg
    16621 gttcaccggc aatcagattg agaaaacgca ccatcgccgc ttcggcgtcg agcatccctt
    16681 catccatgtt gatatcgata atctgcgcac cgttttccac ctgctgacgc gccacatcca
    16741 gcgcctcgct gtatttctct tctttgatca ggcgcttgaa cttagcggaa ccggtgacgt
    16801 tggtgcgttc acccacgttc acaaacaggc tatcttcgcc aatgttcagc ggctccaggc
    16861 cggacaaacg gcaggctacg gggatttccg gcagtttgcg cggcgctaat ccttctactg
    16921 cacgactcat cgcggcaata tgttgtggcg tggtgccaca gcagccgccg acgatattga
    16981 gaaagcccgc ttgcgcccat tcacgtatct gttttgccat cgtgtcggcg tcgagatcgt
    17041 actcaccaaa ggcgttgggt agcccggcgt tcgggtgcgc tgtgacgtag cattccgcaa
    17101 tccgtgataa ttcctgcacg tactggcgca gttcgtctgg ccccagcgca cagttaaggc
    17161 cgaaagtcag cgcttcggcg tggcgcagag agttataaaa cgcttcggtg gtctgcccgg
    17221 agagcgtgcg cccggaggcg tcggtgatgg tgccggagat cataatcggc agctcaacgc
    17281 ccagcgcttc aaactccgtt ttcaccgcaa agaccgccgc tttggcgtta agggtgtcga
    17341 aaacggtttc aatcagaatc aggtcggcac caccttccac cagcgctttg gtggactctc
    17401 gataagccgc caccagcccg tcaaaagtga tattacgaaa tgccggatcg ttgacatccg
    17461 gagaaataga cgccgtgcgg ttggtcgggc cgagaacacc ggcaacgtag cgcggtttct
    17521 ccggcgtgcg cgcggtccac tcatcagcac aggcgcgcgc cagtttcgcc gccgcaaagt
    17581 tgatttccgc cgacaggaat tccatctggt aatccgccat cgcaatggtt gtggagttga
    17641 aggtgttggt ttcgatgata tccgcgcccg cttcaaagta ggcgttgtgg atagcggcga
    17701 tcacttccgg tttgctgagc accagcaggt cgttgttgcc tttgagatcg catggccagt
    17761 cggcaaagcg ttcaccacga aaatcggctt cgttcagtcg atagctctgg atcatggtgc
    17821 ccataccgcc gtccagcacc agaatacgtt catttaactg cgcacgcagt tgttccactt
    17881 tgctgctcac acttgctccc gacacgctca acccagattt aataaaaatt caacaaacca
    17941 tactggcata aacgcatctg tggtaaaagc gaccaccacg caacatgaga tttgttcaac
    18001 attaactcat cggatcagtt cagcaactat tgcattagct aacaataaaa atgaaaatga
    18061 tttccacgat acagaaaaag gagactgtca tggtcgcacc cattcccgca aaacgcggca
    18121 gaaaacccgc cgttgccacc gcaccagcga ctggacaggt tcagtcttta acgcgtggcc
    18181 tgaaattact ggagtggatt gccgaatcca atggcagtgt ggcactcacg gaactggcac
    18241 agcaagccgg gttacccaat tccacgaccc accgcctgct aaccacgatg cagcaacagg
    18301 gctttgtccg tcaggtcggc gaactgggtc actgggcaat cggcgcacac gcctttatgg
    18361 tcggtagcag ctttctccag agccgtaatt tgttagcgat tgttcaccct atcctgcgca
    18421 atctaatgga agagtctggc gaaacggtca atatggcggt gcttgatcaa agcaatcacg
    18481 aagcgattat tatcgaccag gtacagtgta ctcacctgat gcgaatgtcc gcgcctatcg
    18541 gcggtaaatt gccgatgcac gcttccggtg cgggtaaagc ctttctggcc caactgagcg
    18601 aagaacaggt gacgaagctg ctgcaccgca aagggttaca tgcctatacc cacgcaacgc
    18661 tggtgtctcc tgtgcattta aaagaagatc tcgcccaaac gcgcaaacgg ggttattcat
    18721 ttgacgatga ggaacatgcg ctggggctgc gttgccttgc agcgtgtatt ttcgatgagc
    18781 accgcgaacc gtttgccgca atttcaattt ccggaccgat ttcacgtatt accgatgacc
    18841 gcgtgaccga gtttggcgcg atggtgatta aagcggcgaa ggaagtgacg ctggcgtacg
    18901 gtggaatacg ctgacttttt cgcctctgat ttatacgaat cgaacgctaa accgctgctt
    18961 gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt gtagtgcgcg
    19021 ccagtaatca gcgcggaaca agtcggcgtg catctcttca aatagcggac caatacgcgg
    19081 gtcggcacat aaccagtgac ggaactcttc cgggaaaaca tcgcccggcg agacgctgta
    19141 ccacggttcg ctggcaagtt cgtcctccgg gtagcgcggc ggcgggatgt cgcggaagtt
    19201 cacttccgtc atgtagcaaa tttcgtcgta atcataaaac accacgcgcc cgtgacgggt
    19261 gacaccgaag tttttaaaca gcatgtcgcc agggaaaata ttagcggcgg caagctggcg
    19321 aatagcgtta ccgtattctt caatggcatc gcgcaactgc tgaccttcca cttgttccag
    19381 ccagatattg agcggcacca tccgccgctc aatataaaga tggcgaatca caatttgttc
    19441 gccgagatcg gtgatttttt ccgctgcttc ctggagcagt aatgccatta atgccgggga
    19501 aatatgccgc ttctccagca caaagttttc aaactcctgg gtgtccgcca ttcggcccac
    19561 gcgatcgtgc tctttcacca gttgatagca ggcacgaacg tgagcggcag acatctcttt
    19621 ctgcggcgcg aacttgtctt tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa
    19681 caccagcatc accatgccac gaatacccgg cgcttcaatg aactgctcat tacagccctg
    19741 tagataaacg agatattcgc ggtagctttc ggttttggcg tgcttctggc agccgatagc
    19801 catatacaat tcagcggtgg ttttacctgg cagaatttcc cgtagccact cgaccagcgc
    19861 tgcgggcagc ggtgcataaa ccataaaata agaacgcgca aagccaaaaa caatgctcgc
    19921 ttcggcggtg gtcgtcaggc aggtatcaat aaataactcg ccgtcgtccg tctggtggat
    19981 cggcagcaaa aatggcaatg tgccggaagg cgtgatcaga ttgcctacca gccaggcggc
    20041 tttattgcgg taaaacagtt cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt
    20101 ccccagcgtt tccgtcagat ggcgaacgat gtaatgaatg tcacggcttt tattctgcca
    20161 acgcagacgc agcggcagat cgctgataac gcgcatcagc agagattccc agccgtgatc
    20221 ggggtgaaag tctttcgcca gcgggcgggg aatggtacga aaacggcgct ctggctggga
    20281 gctaaagata aaaagccgct cgggagtaag cgagcggtgg tcaaataacc tacagtacac
    20341 ggagttgaaa aagctctccg caatctcgaa gcgcgggtaa tccggcaaca gccgcgtgta
    20401 atgctctttg acgcgcagta aaaatgccgc gtccgtgctt tggccgttgg taatgcagcg
    20461 cagttgctcc acgaccagac ccacgtgatg atcgtaaaga tgaatacggt ttttcatcgc
    20521 ctgctggaca gcatgccagt cggcttgctc gaaacgctgc tgcgccccgg aagtcacttc
    20581 gaggaatcga ccatactgag cgtcgaaacc ttgcaaaatg gtttgtgcaa tcagtaattc
    20641 caggccacgc ggcatttgtt gccctcgtcg ggagcagaga attgccggat aagacgcgta
    20701 agcgtcacat caggcgctga ttgccggatg cggcgtagac gccttatccg gcctacgttc
    20761 ggcaacggct gtaggcatga taagacgcgc aagcgtcgca tcaggcgctg attgccggat
    20821 gcggcgtgaa cgccttatcc ggcctacagt cagcaacgtt tatcggttgc ttagaactgc
    20881 gattcttcgg tggagccagt cagtgcggta accgacgacg tgccgccctg aataatggtc
    20941 gtcactttat cgaagtaacc tgtacccact tcctgttggt gagatacgaa ggtatagcca
    21001 tctttcgcgg cggcaaattc cggctgctgc actttctcaa catagtgctt cataccctcg
    21061 ccctgggcat aggcgtttgc caggtcaaac atgttgaacc acatgctgtg gatacctgcc
    21121 agggtgatga actggaactt gtagcccata tccgacagct gctgctggaa gctggcaata
    21181 gttttgtcgt cgaggttttt ctgccagttg aacgacggcg agcagttata agccagcagt
    21241 ttgcccggat atttcgcgtg gatggcttgt gcaaagcgac gcgccagttc caggtccggc
    21301 gtggaggttt cacaccagac caggtcagca tatggcgcat acgccaggcc acggctgatc
    21361 gcttgctcaa tacccgcatg agtacggaag aagccttcac tggtacgctc gccagtaata
    21421 aattcgctgt catatgggtc gcaatcggag gtaatcagat ccgccgcatc ggcatcggta
    21481 cgagcaacca gcagggttgg aacgcccgtc acatcagctg ccagacgcgc cgcgaccagt
    21541 ttctgaatag cttcctgagt tggcaccaaa actttgccgc ccatgtgacc acatttcttc
    21601 actgatgcca gctgatcttc gaagtgaact gccgctgcac cggcttcaat catcgctttc
    21661 atcagttcaa aggcattcag tacaccgcca aaaccggcct ccgcatcggc gacgatcggc
    21721 aggaagtaat cgacatagcg cggatcgccc ggctcaatgc ccgcggacca ttggatctga
    21781 tccgcacggc ggaaagtatt gttgatccgc tccaccaccg ctggcaccga gtttgccgga
    21841 tagagcgact gatccggata catgctggcc gccaggttag cgtccgccgc tacctgccat
    21901 cccgacagat agactgcttc aatacccgct ttcgcctgtt gcagcgcctg accgccagtc
    21961 agtgcaccga ggctgttgat gtagcctttt ttcgactcgc cgtgcagcag acgccacatt
    22021 ttcgccgcgc ccagttgcgc cagggtgcat tccggattga ctgaaccgcg taatttcacc
    22081 acatcttccg cgctgtacgg gcgagtaatg ccttcccaac gcggttgagt ccactctttc
    22141 tgtaattctt caatttgttg tgtacgggtt ttcatgtgca gatgctccat agttatgtgg
    22201 tggtttacgc taacaggcgg tagcctggca gggtcaggaa atcaattaac tcatcggaag
    22261 tggtgatctg ttccatcaag cgtgcggcat cgtcaaaacg cccctgggag aaacgttctt
    22321 cgcccagttc gctggcaatg actttcatct cttcgcccag catctggcgg aacaaggctt
    22381 tagtcaccgg tttgccattg ctcaacgttt tttgatgatg gatccactgc cagattgagg
    22441 tacgggaaat ttcagccgtc gccgcatctt ccatcaggcc ataaatcggc acacagccat
    22501 tgccagagat ccacgcttcg atgtactgca cagccacgcg aatgttggcg cgcatacctt
    22561 cttcggtgcg ttcaccatca caaggtgcca gcagctgatc ggcagtaatc ggcgcgtctt
    22621 gttcgcgcat cacttcaagc tgatttttac gggagccgag aatgtcgttg aataccgcca
    22681 ttgccgtatc cgcaaggcct gggtgggcaa tccatgtgcc atcgtgaccg ttattagctt
    22741 ccagcgattt atccgctttt actttgttga gcacctggtt attgcgctct tcatctttgc
    22801 tcggaataaa tgccgccata ccgcccatcg caaaagcacc gcgtttatgg caggttttaa
    22861 tcaacaggcg tgagtaagca ttcaggaagg gtttatccat cgtcactgcc tgtctatctg
    22921 gcaggacgcg atcgggatag tttttcaatg ttttgatata actgaagatg taatcccaac
    22981 gaccgcagtt cagaccaaca atatggtcac gcagcgcgtg aaggatttca tccatctgga
    23041 acacggcggg cagcgtttca atcagcaacg tcgccttgat ggtgccgcgc ggcagattaa
    23101 agcgatcttc tgcatagctg aagacttcgc tccaccaggc cgcttcctgc caggactggg
    23161 ttttcggcag atagaaatag ggaccgctgc cctttgccaa cagtgcctga tagttgtgga
    23221 agaaatagag cgcaaaatca aacaggctac cggggattgc ctcaccacgc caggtgacat
    23281 gtttttccgg caagtgcaga ccgcgtaccc gacaaatcaa aaccgctgga ttgggcttga
    23341 gctgataaat tttgcctgct tcattggtat agctgatggt gccgttaacc gcatcgcgca
    23401 ggttaatttg cccatcgatc actttgttcc agtctggtgc cagtgaatct tcgaaatcgg
    23461 ccataaagac tttcacattg gcgttgagcg cgttgatcac catcttgcgc tcaaccgggc
    23521 cggtaatctc tacccgacga tcttctaagt ccgcaggaat cccgcgaatt ttccagtcag
    23581 catcacgaat ggaagctgtt tccgaaataa aatcaggcaa cgttccgtta tcgatatcct
    23641 gctgctgctg aatgcgcgct gccagaagtt tattgcgttg tggcgtaaaa tgcgtcacca
    23701 gctcagtcag aaattctacc gcttcggcag taagaatatg cttctcctgc tcgccatacg
    23761 gccttgtgaa agccaattca tcggttgttg ttgcctgttc agtcatcgtg cagctcctcg
    23821 tcatggatcc gaaaacttcc ccactgtgaa cgaaggatcg ttgtgcgctt ttcgttcagc
    23881 acaactaaga ctactcattt aaaatgcaaa atcaaaaaca atttccattt taattaaaaa
    23941 tacttgataa cttattgata acaatatgaa taaaatttat tcacctactg aggtgcgttt
    24001 cggaaataaa aaaggcaccc gaaggtgcct gaggtaaggt gctgaatcgc ttaacgatcg
    24061 actatcacag aagattaatc cagcgttggg ttcatgtgcc gtagatcgta tggcgtgatc
    24121 tggtagacgt aatagttgag ccagttggta aacagcaaat taccgtgact acgccagctc
    24181 gctcgcggtg tattttgcgg atcattatgc gggaaatagt tatacggtac atccgggtct
    24241 agtccggctt ccacatcgcg gaaaaattcc tgcgccagcg tttgcgcatc atattcggga
    24301 tggcccgtca caaaggcaat tcgcttatct ttgctggcaa acagataggc atccccttct
    24361 tctgtctccg ccagaatttc cagatcggta taatcacgaa tcaacgctgc cggaaagtca
    24421 gcatagcgcg aatgtggtgc caggaatgaa tcatcaaagc cacgcgtcag aagcgcatga
    24481 ggatggagaa tatgatgctc gtaaacgcca gagagtttgt cggtgcgggt ttgcttagga
    24541 atgccgtaga ggatattgag cgcggcctgg accgcccaac agacaaacag cgtcgaggtg
    24601 acgtgatctt tcgaccactc cagcacctgt ttgatctgcg gccagtaagc aacatcatta
    24661 aactccacca ggcccagcgg cgcgccggta actatcagac catcaaagtt ttgttcctga
    24721 atatcttcaa agttacagta gaagttattc agatgctctg cgggcgtgtt gcgcgattca
    24781 cgggaatcga tgcgcaacag ctgaatatcg acctgcaaag gtgagtttga aagcaggcgc
    24841 agaaactgat tttcagtttc gatcttcttc ggcatcaggt taaggataag cacctttagc
    24901 ggacgaattt cctgaccaga cgcacgagaa gttgtcatca caaagacgtt ttcttcacgc
    24961 aagaaattga cggcgggtag ctcgtccggc acacgaatcg gcataacctg attacctcac
    25021 tacatacgct tatacgttta gacatccaga tagctgaaga taaccagaaa atttgccaat
    25081 gtcgagcctg catattgaag gtgagaaagt ttcaaggaaa aggctacgtt agaatataag
    25141 aatgacgaaa aggagagaaa atggttatta gtattcgccg ctcacggcat gaggaagggg
    25201 aggagctcgt tgcgatttgg tgtcgttctg tcgatgccac tcacgatttt ttatcagcag
    25261 cgtatcgggc cgagctggag gagctagttc gttccttcct gccggaagcg ccgttgtggg
    25321 tcgcggttaa tgagcgggat cggccggttg ggtttatgtt gttaagtggg cagcatatgg
    25381 atgcgctgtt tatcgatcct gatgtgcgcg gctgcggcgt gggtcggatg ctggtggaac
    25441 atgcgctctc gatggcaccg gaactgacaa ccaatgttaa tgagcaaaat aagcaggctg
    25501 ttgggttcta taagaagatg ggttttaagg ttacgggacg ctctgaggtg gacgatttgg
    25561 ggaaaccgta tccattgctg aatctggcat atgtagggga ataagctgcc ccctatcgtg
    25621 ctatcgtgct tgcgtaagct ggagcagacg cgccagcgtc gcatccggca ttttttctgc
    25681 gccaatgcaa aaaggccatc ctttcggatg gcctttcgct taatttgatg cctggcagtt
    25741 ccctactctc gcatggggag accccacact accatcggcg ctacggcgtt tcacttctga
    25801 gttcggcatg gggtcaggtg ggaccaccgc gctaaggccg ccaggcaaat tctgtttcat
    25861 cagaccgctt ctgcgttctg atttaatctg tatcaggctg aaaatcttct ctcatccgcc
    25921 aaaaca
//
LOCUS       DAZKGF010000062        25867 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZKGF010000062 DAZKGF010000000
VERSION     DAZKGF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.062"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2783
                     /locus_tag="ACHOQV_002374"
     CDS             <1..2783
                     /locus_tag="ACHOQV_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9630740.1"
                     /translation="NDGHRVDWMVNGLAGDALHKIGKGTLVVAGSGENPGTLNTGDGT
                     VILAQKADAAGRVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDIA
                     FNRLAAADEGAVLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFIM
                     DGGADISGSFTQQGGGLYIQGHPVVHAVSSEAVAAALRKQGDNSVLTQPVSFTQKDWE
                     SRTFSIGQLKLKEASFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVK
                     GTSAVGGASGSSTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRLT
                     SSALTVSDGGRLHVKGGLETDGGVALDRGTLLVDGGSVRNDVYERLLAWSEERGGLNG
                     SGEYDFMTGAAGLLRSYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGE
                     FHTLTAGSMDISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIP
                     LVTAPAGTGADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVATRQ
                     DAETPNPVEAVSPLPSPVSAPSSAPEASVTDVLTGENAGESGEYRDETRWLLTGYRST
                     VNSSAVRDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGF
                     SDNYTHVQVGADKKHELDGLDLFTGVTMTYTYSNAGSDTFSGKTKSVGAGLYASALFD
                     SGAHIDLIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTEDAWIEPQAE
                     LVYGAVSGKQFSWKDQGMSLSMKDKDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQ
                     FDLLANGETVLRDASGEKRIKGEKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYNV
                     DNAVNASFRYSF"
     gene            complement(3351..6023)
                     /gene="rcsD"
                     /locus_tag="ACHOQV_002375"
     CDS             complement(3351..6023)
                     /gene="rcsD"
                     /locus_tag="ACHOQV_002375"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFP9630741.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTTPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLTNQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            6762..7892
                     /gene="ompC"
                     /locus_tag="ACHOQV_002376"
     CDS             6762..7892
                     /gene="ompC"
                     /locus_tag="ACHOQV_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP9630742.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNGTYVSDNNVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8004..9059
                     /gene="apbE"
                     /locus_tag="ACHOQV_002377"
     CDS             8004..9059
                     /gene="apbE"
                     /locus_tag="ACHOQV_002377"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFP9630743.1"
                     /translation="MEISFTRVALLAAALFFVGCDQQPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGKPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KTWMSPQFKSFLISEKN"
     gene            9133..10197
                     /gene="ada"
                     /locus_tag="ACHOQV_002378"
     CDS             9133..10197
                     /gene="ada"
                     /locus_tag="ACHOQV_002378"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFP9630744.1"
                     /translation="MKYATCLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSIINAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVRDVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            10197..10847
                     /gene="alkB"
                     /locus_tag="ACHOQV_002379"
     CDS             10197..10847
                     /gene="alkB"
                     /locus_tag="ACHOQV_002379"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFP9630745.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            10923..12566
                     /gene="yojI"
                     /locus_tag="ACHOQV_002380"
     CDS             10923..12566
                     /gene="yojI"
                     /locus_tag="ACHOQV_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFP9630746.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            12784..14430
                     /gene="mqo"
                     /locus_tag="ACHOQV_002381"
     CDS             12784..14430
                     /gene="mqo"
                     /locus_tag="ACHOQV_002381"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFP9630747.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(14580..15089)
                     /gene="eco"
                     /locus_tag="ACHOQV_002382"
     CDS             complement(14580..15089)
                     /gene="eco"
                     /locus_tag="ACHOQV_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFP9630748.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(15203..15367)
                     /locus_tag="ACHOQV_002383"
     CDS             complement(15203..15367)
                     /locus_tag="ACHOQV_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630749.1"
                     /translation="MHTPIGVKPVAGSKEWQEAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCFLIRI"
     gene            15476..15970
                     /gene="napF"
                     /locus_tag="ACHOQV_002384"
     CDS             15476..15970
                     /gene="napF"
                     /locus_tag="ACHOQV_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFP9630750.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLLFTIGEAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNACGACAASCPVSAITAEY
                     LHVH"
     gene            15960..16223
                     /gene="napD"
                     /locus_tag="ACHOQV_002385"
     CDS             15960..16223
                     /gene="napD"
                     /locus_tag="ACHOQV_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFP9630751.1"
                     /translation="MYTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            16220..18706
                     /gene="napA"
                     /locus_tag="ACHOQV_002386"
     CDS             16220..18706
                     /gene="napA"
                     /locus_tag="ACHOQV_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFP9630752.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPLSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            18713..19408
                     /gene="napG"
                     /locus_tag="ACHOQV_002387"
     CDS             18713..19408
                     /gene="napG"
                     /locus_tag="ACHOQV_002387"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFP9630753.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            19395..20258
                     /gene="napH"
                     /locus_tag="ACHOQV_002388"
     CDS             19395..20258
                     /gene="napH"
                     /locus_tag="ACHOQV_002388"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFP9630754.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPASVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            20255..20704
                     /gene="napB"
                     /locus_tag="ACHOQV_002389"
     CDS             20255..20704
                     /gene="napB"
                     /locus_tag="ACHOQV_002389"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFP9630755.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            20714..21316
                     /gene="napC"
                     /locus_tag="ACHOQV_002390"
     CDS             20714..21316
                     /gene="napC"
                     /locus_tag="ACHOQV_002390"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFP9630756.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            21329..21952
                     /gene="ccmA"
                     /locus_tag="ACHOQV_002391"
     CDS             21329..21952
                     /gene="ccmA"
                     /locus_tag="ACHOQV_002391"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFP9630757.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAENKIRRISLTQTRGE"
     gene            21949..22611
                     /gene="ccmB"
                     /locus_tag="ACHOQV_002392"
     CDS             21949..22611
                     /gene="ccmB"
                     /locus_tag="ACHOQV_002392"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFP9630758.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            22653..23390
                     /gene="ccmC"
                     /locus_tag="ACHOQV_002393"
     CDS             22653..23390
                     /gene="ccmC"
                     /locus_tag="ACHOQV_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFP9630759.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            23387..23596
                     /gene="ccmD"
                     /locus_tag="ACHOQV_002394"
     CDS             23387..23596
                     /gene="ccmD"
                     /locus_tag="ACHOQV_002394"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFP9630760.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVILVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            23593..24072
                     /gene="ccmE"
                     /locus_tag="ACHOQV_002395"
     CDS             23593..24072
                     /gene="ccmE"
                     /locus_tag="ACHOQV_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFP9630761.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            24069..>25867
                     /gene="ccmF"
                     /locus_tag="ACHOQV_002396"
     CDS             24069..>25867
                     /gene="ccmF"
                     /locus_tag="ACHOQV_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFP9630762.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAMLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRNGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGA"
BASE COUNT         5880 a         6366 c         7334 g         6287 t
ORIGIN      
        1 tgaacgacgg gcaccgtgtt gactggatgg tgaacggact tgcgggagac gcacttcata
       61 aaattggtaa gggaacacta gtggtggccg gcagtggtga aaatcctggc accctgaata
      121 cgggggacgg aacggtcatt ctggcacaga aggctgatgc tgcaggcagg gtccgggcat
      181 tcagtgaagt cagtattgtc agcggacgac cagttgtggt gctgcaggac agtcatcaga
      241 ttgaagggga ccgcattcgc tggggatacc ggggaggtac gcttgatatc aacggaaatg
      301 acatagcctt caaccgcctg gctgcggctg atgagggggc agtactcaca agccgggcgc
      361 gtcctgccac agtccgtctg gatttcagcc agtcagggca gaaggccgtg atgtggcatg
      421 gtcattttac cggtaacctc agcgtcctga acaacacgtc atcagcggtg gattttatca
      481 tggatggcgg agcggatata tccggcagtt ttacacagca gggcggggga ctgtatatcc
      541 aggggcatcc ggttgttcat gccgtgagca gtgaggcggt tgcggctgca ttgcgaaagc
      601 agggtgataa ttctgttctg acgcagccgg tatcatttac ccagaaggac tgggagagca
      661 ggacgttcag catcgggcaa ctgaaactga aggaggcgtc attcagcctt tcccgcaatg
      721 cgacactgac gggggatatt gatgctgata atgccaccat ggtgctggga agtgactcgc
      781 tgtatctgga tatgaaggac ggcacgggga gcagcagtgc gccggttaag gggacgtctg
      841 ccgtgggcgg tgcatccggt agcagtacat ttcgcgggaa tgtgaatatg cgtcattctg
      901 ccctgacggt ccgcgaccat tttactggca gtataacggc atcggacagc cggatagctg
      961 tgagttcaga gaatgtgcgt ctggaaggga attcccgcct gacctcttct gcacttactg
     1021 tcagtgacgg cgggcgttta catgtgaagg ggggactgga gacagatggt ggtgtggcac
     1081 tggacagagg aacccttctg gtggacgggg gaagtgtcag gaatgatgta tatgaacgtc
     1141 tgctggcctg gtcagaggag cgtggcgggc tgaacggaag cggtgagtat gattttatga
     1201 cgggggcagc tggtctcctg cggagttatg tcagagggag tgcaggtaat gtgaatctgc
     1261 agaatgcggc ctggatgatg acgggaaact catccgtgaa gcatcttgag tcatcgggaa
     1321 gtgctctgta tttcagcagg ccgggcgggg agttccatac cctgacggca ggcagtatgg
     1381 atatcagcga cagtgttctg gtgatgcgga cggaccttaa ccactcagac cagcttcggg
     1441 tgacagaatc gctgcggggt aagaataacc tgttgttggt tgattttact gagcgttctg
     1501 acgggcagaa ggcgctgaat atccctctgg tgacggcgcc ggccggtacc ggtgcggatg
     1561 tgttcagtgt gaaaacgcgt gataccggct tcagtcatat cacacctgtt gtcagggctg
     1621 aacaggggac gggcggcacg gcctggcagc tgaatgttgt gcagccggaa actgccgcag
     1681 agcctgtggc gacgcggcag gatgcagaga cgccgaatcc tgtggaagct gtatcaccgc
     1741 tcccttcacc ggtttctgct ccgtcatcgg ctccggaggc gtcagtgacg gatgtactga
     1801 ccggagagaa tgcgggggaa tcaggtgagt accgtgatga gacccggtgg ctgctgacag
     1861 gataccggag tacggttaat agctccgcgg tcagggatgc ggggatgctg atgtcaatgg
     1921 ggcaccgtaa cttcattaat gaggtcaaca acctgaacaa acgtatgggt gacctgcggg
     1981 acatcaacgg cgaggccggt gcatgggccc gtatcatgag cggaaccggc tctgccggcg
     2041 gtggcttcag tgacaactac acgcacgttc aggtgggtgc ggataaaaaa catgaactgg
     2101 acggtctgga cctcttcacc ggggtgacca tgacatatac ctacagcaac gccggcagcg
     2161 acaccttcag tggtaaaacg aagtcagtgg gtgcaggtct gtacgcttca gccctgtttg
     2221 attccggggc ccatatcgac ctgattggta agtatgttca ccatgataat gagtatacgg
     2281 cgtcctttgc cggactcggc acaaaagact acagctctca ttcctggtat gccggtgctg
     2341 aagtgggtta ccgctatcat gtcacagagg atgcctggat tgagccacag gcggaactgg
     2401 tctatggtgc tgtatccggt aaacagttca gctggaagga ccaggggatg agcctgtcca
     2461 tgaaggacaa ggactacaat ccgctgattg gccgtaccgg tgtggatgtg ggtaaatcgt
     2521 tctccggtaa ggactggaaa gtgacggcac gtgctggtct tggctatcag tttgacctgc
     2581 tggcgaacgg agaaacggta ctgcgtgatg catccggaga gaagcgtatt aagggcgaga
     2641 aagacggccg tatgctgatg tctgtgggtc tgaacgcaga ggtccgtgac aacatccgtt
     2701 ttggtctgga gtttgagaaa tcagcctttg gtaagtacaa cgttgataat gctgtcaacg
     2761 ccagcttccg ttattcattc tgacggttgt tactgaaggg gattatggcc tgacggctcc
     2821 ctgtaaataa agacaggcac agaagattgt gagttactta gtgttccacc aaaaagtaag
     2881 gtcgcaactc tgtgcctgct tcgtaagtgt cagtgagggc catatggtca taactgaagc
     2941 atccgacgac gtggtgtcta ccagagtgtc agatatgcag gaaaatatga gttccgacag
     3001 aaatgcagta aaaaaaagga gtctcgcact cctctccaga aaactccagg gctgtaagtt
     3061 gtattaaaaa gctgattttt ctttagcgat acagacctca ataagtataa cacccttcag
     3121 ttactgtagt tatgcacagg tgttattttt aacagtgtgg ataaacagtg tcgattattc
     3181 agattggtta tggggcatgt atgggacatt tttaatcggc gaaatacgtc gaagttcgtc
     3241 gatatggtaa acgaatcatc tatccaaccc ttgaaaaacg gcgctcctgg acgatcttcg
     3301 tcgattttta aaatgcaaca agtacgttgt tcatgtaata ggctaccttg ctacagcaag
     3361 ctcttgacat aactgtcaat gtcgctgatg tatttttcta ttcctggaac atccttctca
     3421 cgaatcagat gttccagcgt ttcacataac tgcttgccgg gtaccagatt tagcatggca
     3481 aatacgcctt taagacgatg agccgtttgg gctaacgcag caaagtcact ggtcgctgct
     3541 tcagtataca gcctcttaac atcatccggt actgtgtcta caaagagcgc ataatagccg
     3601 ctggcatgga gttgcgcatt ttcatctccg cccagaggcg attctgtcac ctcttcctgc
     3661 gccagttgca cttcaattaa ttgtaagacc gcttcctgca tagcgttgct catattgaag
     3721 ttgacgcaca attgaccagg cccaatttcc cgtacgccag actcatcatc gcttaaaagc
     3781 aagccagagg cagtaagatt agacggatta tccgttaaaa agatatcata atcttgacta
     3841 attaatcttt catcgggtgt gatacaggtt gcaccccaat tttctaactg gcgagtgaca
     3901 atattccgaa tttctgccga agtaacatcc accattacgc agacatcatc cagtaaacgc
     3961 tcttcttcct cttcaacttc cgggtcagct gcgagcattt tgatatgcac agagtagcga
     4021 gtaccaagcc catcccgcgt tttgatgttt aaatgaccgc ccagtttacg cgccagttga
     4081 tcgctcagcc agaatgccag cgggtccgcc ttgccatagc gatcgttttg ggtctggttg
     4141 ataaacggga agtgcaaatt atccatttca tgaatgctta cgccttctcc agtgtccaga
     4201 atacggaacg tcaggcggtc ttcggaggac tcatcctgat caacctcaag ggtgattttt
     4261 cccaattgcg ttgaggtcac ggcatattgc atcagtaaca gcaaaatacg tcgtaaggca
     4321 tcgcgatcgc cgcggcgcat gtcgtgtgct ttcagatgat tgttaatcag cagttgcaga
     4381 cctttacgct tgatggcagg caacactgaa ggcacaactt catcaattaa atcctgcacg
     4441 gagaacagca ccgtctcact tttccagcta tcgtccgcaa gcatgttcgc taactgtatt
     4501 tcatcgacca gccgcaccag cacatccgcc tgattcgtca gttgtttgcc ttccggggcg
     4561 ttgagtttag ccgcgctctc cgccagggac tgtgcgggtt ctttcagcgc atcgccaatg
     4621 tttttcataa aggtcatccg cccctgctgg tttttctcat acagacgctg cgcctgcttg
     4681 agtttcttgt ttaccagcac ttcgcgatcc tgatcgcgaa taatgaaaat ttgtgtgcgc
     4741 ggcgcaacct ggctgcggaa catgcggatc tcatacagct cgttattgat cgtcgcctga
     4801 ataatcccct gatgctgttc cgccatggtg gtgatgtttt gcagattcaa atgcggcagc
     4861 aaatgatcgg caattttgtt acttatgaca gtgcggttcg attcctgatc gtgaaccagc
     4921 aggccgagcg gcagcagtga gactatctct tcattgattg cccgtaaaat gcgcaattcg
     4981 ttattgaccg ccgtgttggg tagactttct gtactgcgac tggagaaatg gcgaaatgtg
     5041 gtatagccaa ataacgccag cgccagcaaa ccgatgttca gcagcagtgg cagcagaatg
     5101 ttttgcaacg tatccagcaa taaggtgcca taaggaacct gccagaccag gcgcatatcg
     5161 gtagagttga gtgccgagga gatttcaatc ttcgtactgt taaagtggat actgacgcta
     5221 tccgtccctt ctttctcatt atcattgtct cccgttgccg tcgcgtctgg ctcaaggcgg
     5281 aaactgtcca gcggcatacc cggtgggatc aaatcattaa tcggcagatc aaaagccaca
     5341 accgttgcca gatgtcccgg ctggttgaac gtggtacgca aggtaaagta atgaccgttc
     5401 tgccaggcca ggcggcgcat gttagaaaag ctttcgcgtt catcgagggc gttggcctgt
     5461 tgcaacatct ctgcacgacg tgaatcaaca atgtcgctga cggtcgattc tttaaatccg
     5521 gaggtgagat ctttgagggg cagggttgag atcagcacca gactgttatc ctgaccattc
     5581 aggtaataca tcgaccacgg tacattttct gcgccccaca atgtatccag ataagtggac
     5641 atccgttgag tcatctcaag cgttgagttg tcgtgagagc caaagattaa cgcttccgtt
     5701 ttgcggcgtg gtttctccag atagtagaca tcctgtttca ggcgcgtctc ttgtaaacct
     5761 tcgccggagg agggggtggt cgtcgcggca atgttgtcgt agatctgcca ggtcacgtaa
     5821 cgccaggtat cgacgcgctt ttggatagcg tgggtaatgt cgacaatctg gtaactttta
     5881 tctttcagcc aggcgttaac ggcgctttgt accattacac ccatcgtcac cagtaacaca
     5941 atgatcaaca gtaaaaagaa gcgggtaatg ctccccggta ggagtgaaaa gcgggtcgtg
     6001 gccgttgtct ctttctgacg cattcgtgtt tatgacctgt taaaacttcg cgaagtgata
     6061 gggcagtata aagggtacag tgtgaaattc cagactctta ccggattgat gattctctat
     6121 tctcccgtga tatttatcat cctgcattgg gggaaataga tgtacaagcg ccattttgct
     6181 gtacaaataa cctacaaaaa gcctaacgaa attaattgca aataaacgaa gattgctgga
     6241 aattatgctg atgttaatta tttgtgaaat agttaacaag cgttatagtt tttctgtggt
     6301 agcacagaat aatgaaaagt gtgtaaagaa gggtaaaaaa aaccggatgc gaggcatccg
     6361 gttgaaatag gggtaaacag acattcagaa atgaatgacg gtaataaata aagttaatga
     6421 tgatagcgga agatattcta gttgcgagtg aaggttttgt tttgacattc agtgctgtca
     6481 aataattaag aataagttat tgattttaac cttgaattaa tattgcttga tgttaggtgc
     6541 ttatttggtc attccgcaat aatcttaaaa agttcccttt catttacatt ctgaaacatc
     6601 tatagcgata aatgaaacat cttaaaagtt ttagtatcat attcgtgttg gattattctg
     6661 catttttggg gagaatggac ttgccgactg attaatgagg gttaatcagt atgcagtggc
     6721 ataaaaaagc aaataaaggc atataacaga gggttaataa catgaaagtt aaagtactgt
     6781 ccctcctggt cccagctctg ctggtagcag gcgcagcaaa cgctgctgaa gtttacaaca
     6841 aagacggcaa caaattagat ctgtacggta aagtagacgg cctgcactat ttctctgacg
     6901 acaagtctgt agatggcgac cagacctaca tgcgtcttgg cttcaaaggt gaaactcagg
     6961 ttactgacca actgaccggt tacggccagt gggaatatca gatccagggc aacagcgctg
     7021 aaaacgaaaa caactcctgg acccgtgtgg cattcgcagg tctgaaattc caggatgtgg
     7081 gttctttcga ctacggtcgt aactacggcg ttgtttatga cgtaacttcc tggaccgacg
     7141 tcctgccaga attcggtggc gacacttacg gttctgacaa cttcatgcag cagcgtggta
     7201 acggcttcgc gacctaccgt aacaccgact tcttcggtct ggttgacggc ctgaactttg
     7261 ctgttcagta ccagggtaaa aacggtagcg tagatggcga aggcatgacc aacaacggtc
     7321 gtggtgctct gcgtcagaac ggcgacggcg ttggcggttc tatcacttat gattacgaag
     7381 gcttcggtat cggtgctgca gtgtccagct ccaaacgtac tgacgctcag aatggtacct
     7441 atgtaagcga caataatgtg gtccgtaact acatcggtac tggtgaccgt gctgaaacct
     7501 acactggtgg tctgaaatac gacgctaaca acatctacct ggctgctcag tacacccaga
     7561 cctacaacgc aactcgcgta ggttccctgg gttgggcgaa caaagcacag aacttcgaag
     7621 ctgttgctca gtaccagttc gacttcggtc tgcgtccgtc cctggcttac ctgcagtcta
     7681 aaggtaaaaa cctgggtgtc atcaatggtc gtaactacga cgacgaagat atcctgaaat
     7741 atgttgatgt tggtgcgacc tactacttca acaaaaacat gtccacctat gttgactaca
     7801 aaatcaacct gctggacgac aaccagttca ctcgtgacgc tggcatcaac actgataaca
     7861 tcgtagctct gggtctggtt taccagttct aatctcgatt gatatcgaac aaagggcctg
     7921 cgggcccttt tttcattgtt ttcagcgtac aaacacagtt ttttggtgta ctcttgcgac
     7981 cgttcgcatg aggataatca cgtatggaaa taagctttac ccgcgtggca ctgctggctg
     8041 ccgcgctctt ctttgttggt tgcgatcaac aaccacaacc cgccaaaacc cacgctactg
     8101 aagttaccgt tcttgaaggc aaaaccatgg gtaccttctg gcgtgccagc atcccgggca
     8161 ttgacgccaa acgcagtgcc gaacttaaag aaaagattca gacccaactg gacgctgacg
     8221 atcagctgct ttcgacctat aaaaaagatt ccgcgctgat gcgctttaac gactcgcaaa
     8281 gtttgtcgcc gtggccggta agtgaagcga tggccgatat cgtcactacc tcgctgcgca
     8341 ttggcgcgaa gaccgatggc gcgatggata taaccgtcgg gccgctggtg aatctgtggg
     8401 gctttggtcc ggaacaacaa ccggttcaaa ttccgagcca ggaacagatc gatgcgatga
     8461 aagccaaaac cggcttacag cacctgacgg tcattaatca gtcgcatcag caatatctgc
     8521 aaaaagacct gccggattta tatgtcgatc tctccaccgt cggcgaaggt tatgcggcgg
     8581 atcacctggc acgcttgatg gagcaggaag ggatttcccg ctatctggtg tcggtgggcg
     8641 gcgcgctgaa cagccgtggt atgaacggtg ctggcaagcc gtggcgggta gcgattcaaa
     8701 agccaaccga taaagaaaac gcggttcagg ccgtggtgga tatcaacggt catgggatta
     8761 gtacctctgg cagctaccgt aactattacg aactggacgg caaacgtctt tctcatgtta
     8821 tcgatccaca aaccgggcgt cctatcgaac acaatctggt atccgtgacg gtgattgccc
     8881 cgacggctct ggaagccgat gcctgggata ctggcttaat ggtactcgga ccggagaaag
     8941 ccaaagaggt tgttcgccgg gaaggactgg cggtctacat gatcaccaaa gagggcgata
     9001 attttaaaac ctggatgtcg ccgcagttta aaagcttcct tatcagcgaa aaaaattaaa
     9061 gcgcaagatt gttggttttt gtgcgatggt gaccgggcag actaaaggct atccttaacc
     9121 agggagctga ttatgaaata tgccacatgc ttaactgacg atcaacgctg gcaatctgtc
     9181 ttagcccgcg actcgaatgc cgacggcgaa ttcgttttcg ccgtgcgcac cacgggtatc
     9241 ttttgccgtc cgtcttgccg agccagacat gccttgcggg aaaatgtctc cttctacgca
     9301 aatgccagcg aggcgcttgc cgctggcttt cgtccctgca aacgttgtca gccagacaaa
     9361 gccaatcccc tgcaacatcg ccttgataaa attacccacg cctgccgact tctggaacag
     9421 gaaacgcctg taacgctgga agccttagcc gaccaggtgg cgatgagtcc atttcatttg
     9481 catcggttgt ttaaagcgac taccggaatg acgcctaaag cctggcaaca ggcctggcgc
     9541 gctcgccgtt tgcgcgaatt gctggcgaaa ggggagagcg tgactacatc tattattaac
     9601 gccggattcc ccgacagcag cagttactac cgcaaagccg acgaaacgct gggcatgacg
     9661 gctaaacaat tccgtcacgg tggcgaaaat ctggcggtgc gttacgcgct ggctgattgt
     9721 gagctgggcc gttgcctggt ggcagaaagt gagcggggga tttgcgcgat attactgggc
     9781 gatgatgacg cgacactaat cagcgagtta cagcagatgt ttcccgctgc cgacagcgcg
     9841 cctgctgatc ttacgtttca gcaacatgtg cgtgacgtga tcgccagcct caatcaacgc
     9901 gatacgccgc tgacgttacc gctggacatt cgcggcactg cttttcagca acaagtctgg
     9961 caggcactgc gcacgatacc ttgcggtgaa accgtcagtt atcagcaact ggctaatgcc
    10021 atcggcaaac cgaaagcggt acgggccgtt gccagcgcct gtgccgccaa caagctggct
    10081 atcgtaatac cctgtcatcg ggtggtccgt ggtgatggca cactttccgg ttaccgctgg
    10141 ggcgtgtcgc gcaaagcgca actgctgcgc cgcgaagctg aaaatgagga aaggtaatgt
    10201 tggatctgtt tgccgatgct gaaccgtggc aagaaccact ggcggctgga gcggtgattt
    10261 tgcaccgttt tgcttttaac gctgcggagc aactgatccg cgatattaat gacgttgcca
    10321 gccagtcgcc gtttcgccag atggtcactc caggcggata taccatgtcg gtggcgatga
    10381 ccaactgtgg gcatctgggc tggacgaccc atcggcaagg ttatctctat tcgcccattg
    10441 atccgcaaac aaataaaccg tggcccgcca tgccgcagag ttttcatcat ttatgtcaac
    10501 gtgcggctac ggcggcgggc tatccagatt tccagccaga tgcctgtttg atcaaccgct
    10561 acgctcctgg cgcgaaactg tcgctgcatc aggataaaga cgaaccggat ctgcgcgcgc
    10621 cgattgtttc tgtttctctg ggcttacccg cgatttttca atttggcggc ctgaaacgaa
    10681 atgatccgct aaaaagattg ttgctggaac atggcgatgt ggtggtatgg ggcggtgaat
    10741 cgcggctgtt ttatcacggt attcaaccgt tgaaagcggg gtttcatcca ctcaccaccg
    10801 actgccgcta caacctgaca ttccgtcagg caggtaaaaa agaataaaaa taagaattat
    10861 tattgctgtg cgcgcgaaga tgtttaaact gcgggctgta attcattgtc cgggttttct
    10921 gcatggaact tcttgtactt gtctggcggc agtatcgctg gccatttatc agtgtgatgg
    10981 cgctaagcct cgccagtgcg gcattaggca ttggcttaat tgcttttatc aatcagcgcc
    11041 ttatcgaaac ggcggatacc agtctgctgg tattgccgga gtttctgggg ttattgctgc
    11101 tgttgatggc cgtcactctc ggatcgcaac tggcgctcac cactttgggg catcacttcg
    11161 tttaccgact gcgtagtgaa tttatcaagc ggattctcga tactcacgtc gagcgcattg
    11221 aacaactcgg tagcgcctcg ttgctggcgg gattaaccag cgatgtgcgc aatatcacca
    11281 ttgcttttgt gcgtctgccg gaactggtgc aggggatcat tctcactatc ggttcagcgg
    11341 cgtatttgtg gatgctgtcg ggcaaaatgt tgctggtaac ggctatctgg atggcagtca
    11401 ccatctgggg cggttttgtg ctggtggcgc gggtgtacaa acatatggcg accctgcgtg
    11461 aaaccgaaga taagctgtac acggattttc aaactgtact cgaagggcgc aaagagctga
    11521 ctctgaaccg tgaacgcgcc gagtatgtgt ttaacaacct ctacattcct gatgcgcaag
    11581 agtatcgcca tcatattatt cgcgcagaca ccttccatct tagtgccgtg aactggtcaa
    11641 acatcatgat gctgggcgct atcggcctgg tgttctggat ggcgaacagt ctcggttggg
    11701 ctgataccaa cgttgctgcg acctattcgt tgacgctgtt attcctgcgt acgccgctgc
    11761 tttcggcggt tggcgcattg ccgacgctgc tgacggcgca ggtggcgttt aacaagctga
    11821 acaaattcgc gctcgcgcct ttcaaagcag agtttccgcg cccgcaggct tttcccaact
    11881 ggcaaacgct ggagctgcgt aacgtgacgt ttagctatca ggataacgcg ttttccgttg
    11941 ggccaattaa cctcaccatc aaacgtggcg agctgctgtt tctgattggt ggcaacggta
    12001 gtggaaaatc gacgctggcg atgttgctga cgggcttgta ccagccacaa aatggcgaaa
    12061 tcttgctgga tggcaaacct gtcagcggcg aacaaccgga agattatcga aaactgtttt
    12121 cggcagtgtt taccgatgtc tggctgtttg atcaactgct ggggccggag gggcaacccg
    12181 ctaacccgca actggttgag aagtggctgg cgcagctgaa aatggctcat aagcttgagt
    12241 taagcaacgg gcgtattgtt aacctgaagt tatcaaaagg gcagaaaaaa cgcgtggcgc
    12301 tgttgctggc gctggcagaa gaacgcgata ttatcctgct ggatgaatgg gcggcggatc
    12361 aggatccaca cttccgtcgt gagttttatc aggtgctgct gccgctgatg caggagatgg
    12421 gtaaaactat tttcgccatc agtcatgatg atcattactt tatccacgcc gaccgcctgc
    12481 tggaaatgcg caacgggcaa cttagcgagc tgacaggcga agagcgcgat gctgcttcgc
    12541 gtgatgccgt tgcccggacg gcataatatt acgacgctga cctggcggac tgctgccgcc
    12601 aggttaatat gctaaaaatc ccttcatttc taataaccct ataattaatc aacgaaatta
    12661 taatgtttct aaaattagaa tataatttat aaacattatt taaatgttgt tacttaagtg
    12721 ttaccgttga tgccgcgcaa atctcacctg caataaagcg actaaaagta aggcattaac
    12781 aagatgaaaa aagtgactgc catgctcttc tcgatggccg tggggcttaa tgccgtttcg
    12841 atggcggcaa aagcgaaagc atccgaggag caggaaactg atgtactgtt gattggcggc
    12901 ggcattatga gcgccacgtt ggggacctat ttacgcgagc tggagcctga atggtcgatg
    12961 accatggtgg agcgcctgga gggtgtcgcg caggagagtt cgaacggctg gaacaacgcc
    13021 ggaaccgggc attctgcact gatggaactc aattacactc cgcaaaacgc cgatggcagc
    13081 atcagtattg aaaaagcagt cgccattaac gaagcatttc agatttcgcg ccagttctgg
    13141 gcgcaccagg tcgagcgcgg cgtgctgcgt actccgcgtt catttatcaa taccgttccg
    13201 catatgagct ttgtctgggg cgaggataac gtcaatttcc tgcgcgcccg ttacgccgcg
    13261 ttgcaacaaa gctcgctgtt tcgcggtatg cgttactctg aagatcacgc gcagatcaaa
    13321 gagtgggcac cgttagtgat ggaagggcgc gatccgcaac agaaagtggc agccacgcgt
    13381 acggaaattg gtactgatgt gaactatggc gagatcaccc gccagttaat tgcttccttg
    13441 cagaagaaat ctaatttttc gctgcaactc agcagcgaag tccgcgccct aaagcgtaat
    13501 gacgataaca cctggaccgt cactgttgcc gatctgaaaa atggcactgc acagaacatc
    13561 cgcgccaaat ttgtctttat cggcgcgggc ggtgcggcgc tgaagctgtt acaggaatcg
    13621 gggattccgg aagcgaaaga ctacgccggt ttcccggtgg gcggacagtt ccttgtttcg
    13681 gaaaacccgg acgtggttaa tcaccatctg gcgaaggttt acggtaaagc atccgttggc
    13741 gcaccaccga tgtcggttcc gcatatcgat acccgtgttc tggacggtaa acgcgtcgtg
    13801 ctgtttgggc catttgccac cttctcaacc aaattcctca aaaacggttc attgtgggat
    13861 ctgatgagtt ccaccaccac ctctaacgtg atgccgatga tgcacgtcgg gctggataat
    13921 ttcgatctgg tgaaatatct ggtgagtcag gtgatgttga gtgaagagga tcgttttgaa
    13981 gcgttgaaag agtactatcc gcaggcgaaa aaagaggact ggcgtttgtg gcaagcgggg
    14041 cagcgcgtgc agattatcaa gcgtgatgcc gagaaaggtg gcgtactgcg tctgggtacc
    14101 gaagtggtca gcgaccaaca aggaaccatt gccgcgctgc tgggggcatc gccaggggcg
    14161 tcaaccgccg cgccgattat gttggatctg ctggaaaaag tatttggcga tcgcgtttcc
    14221 agcccgcaat ggcaggctac gttgaaagcg atcgttccgt cttatggacg caagctgaac
    14281 ggtgatgtag cggcaacaga acgcgagttg cagtacacca gcgaagtgct ggggctgaaa
    14341 tacgacaaac cgcaggcagc agatagtacg ccgaaaccgc agttgaaacc gcaacccgtt
    14401 caaaaagaag tggcggatat tgcgttgtga tgatgtgcca catccggcat ggtatgccgg
    14461 atgtggcgta tgcagataag acgcgtcagc gtcgcatcag gcaaccggct cgggcgttag
    14521 atgtcggatg cggcgtaacc gccttatccg acctggcggt tgcagcactt cacagcagtt
    14581 tagcgaacta ccgcgttgtc aattttctct tccgccttcc agacgcggta cttcacatct
    14641 acattgtctg gcgtatacac cacgatcggc agcttgctgt tataacgcag cattccggca
    14701 tcgcccagat acgcggtgac aaatttcttc tctttcttgc catccgggca ggccatcatc
    14761 gtcgaaaccg gggaactgac tttatcaaag acataataat catagcccca gccttccagc
    14821 gttttgcttt ccagcttccc gccgagacga tgcaaattgc aatcgacttc cagcgtctgg
    14881 ccgattaaca gttctacttt cagggtagat tcatcttctt gcggggttaa ctgaatcacc
    14941 tggcgcttca tgcctttttc agcttgtgga taaggtgcga ttttttccag tggctggacg
    15001 ctttctgccg cccaggcgga agtggtggcg aaagcggcaa acaatactgc aggtagaatg
    15061 gtcttcattt ttttgctttc cttcttcatt tataggtttt caccgatgat gtcgctgaag
    15121 gttaacatat tcatcagatt tattaatttg ctttatgaat aacttaaaga acattcagat
    15181 aattttacct gctcttttag tgttaaattc tgatgagaaa acaaaccagc agaaatatct
    15241 ctggcgaatt aatcgctata taaatatgtt tataaccatt tgaaatgtga gcaaaagccc
    15301 gtttttgcca cgcttcctgc cactcttttg atcctgctac aggttttacc ccgatcgggg
    15361 tatgcatctt tgacacatcc tttaatatct tagcggctat aaaaatggct tattaattat
    15421 gcggtttatt tggtcgctct caattttcag agcgcgttaa tgatggaagg tcaatgtgaa
    15481 gattgatgca tcccgtcggg gcatactcac tggtcgctgg cgcaaagcca gtaacggtat
    15541 ccgtccgccc tggtccggtg atgaatctca ttttctgacc cattgcactc gctgcgacgc
    15601 ctgtattaat gcttgtgaaa acaacattct gcaacgcggc gcgggtggct atccgagcgt
    15661 taatttcaaa aataacgagt gcagcttctg ttacgcctgc gcgcaggctt gccccgaatc
    15721 gctattttct ccgcgccaca ccagggcctg ggatttgctg ttcaccatcg gggaggcgtg
    15781 cctggcgtat cagtcagttg aatgccgccg ctgtcaggat agctgtgagc caatggcaat
    15841 tatctttcgc ccgacgctct ccgggatcta ccagccgcaa cttaatagcc aactttgcaa
    15901 cgcatgtgga gcatgtgcag ccagctgtcc ggtatcagcc ataactgcgg agtatctcca
    15961 tgtacactaa ctggcaagtt tgcagcctgg tcgtgcaggc caaaagcgaa cgaatttcag
    16021 acatcagcac ccaactgaac gcctttcccg gctgtgaagt tgctgtcagc gacgcgccga
    16081 gcggccagtt gattgtggtg gtggaagcag aagacagcga aacgctgatc caaaccattg
    16141 agtcagtacg caacgtagag ggcgtgctgg cggtgtcgct ggtttatcac cagcaggaag
    16201 agcaaggtga ggaaacacca tgaaactcag tcgtcgtagc tttatgaaag ctaacgccgt
    16261 tgcggccgct gcggcggctg ccggtctcag cgtgccgggc gttgcccgcg ccgttgttgg
    16321 tcagcaggaa gccattaaat gggataaagc gccgtgccgt ttctgcggta cgggttgcgg
    16381 cgttctggtc ggaacgcagc aggggcgtgt ggtggcctgt cagggcgacc cggacgcacc
    16441 ggttaaccgt ggtctgaact gcattaaggg ctatttcctg cccaaaatca tgtacggtaa
    16501 agaccgtttg acgcagccgc tgctgcgtat gaaaaacggt aaatatgaca aagaaggcga
    16561 atttacccca atcacctggg atcaagcctt cgatgtgatg gaagagaaat tcaaaaccgc
    16621 cctgaaagaa aaagggccgg aatcgatcgg tatgttcggt tctggtcagt ggactatctg
    16681 ggaaggttat gccgcgtcca agctgtttaa agcgggattc cgttcgaaca acatcgaccc
    16741 gaacgcgcgt cactgtatgg cgtcggcggt agttggcttt atgcgtacct ttggtatgga
    16801 tgagccgatg ggctgctatg acgacatcga gcaggctgac gcgtttgtgc tgtggggctc
    16861 caacatggcg gagatgcacc cgatcctctg gtcgcgcatc actaaccgtc gtctctccaa
    16921 ccagaacgtc accgtggcgg tgctttctac ctaccagcat cgtagcttcg agctggcgga
    16981 taacggcatc atctttacgc cgcaatctga cctggtgatc ctgaactata tcgccaacta
    17041 tatcattcaa aacaatgcga taaatcagga cttcttcagc aaacacgtta acctgcgcaa
    17101 aggggcgacg gacatcggct acggtttacg tccgacccat ccgctggaaa aagcagcgaa
    17161 gaatccgggt tctgacgcct ccgaaccgat gagctttgaa gattacaaag ccttcgttgc
    17221 tgaatatacg ctggaaaaaa ccgccgaaat gaccggcgtg ccgaaagacc agttagaaca
    17281 actggcgcag ctgtatgccg atccgaacaa gaaagtcatc tcctactgga cgatgggctt
    17341 caaccagcat acccgtggtg tgtgggccaa caacctggtc tacaacctgc acctgctgac
    17401 cggcaaaatt tcccagccgg gttgcggtcc gttctccctg accgggcagc cttccgcgtg
    17461 tggtactgcg cgtgaagtgg gcacctttgc tcaccgtctg cctgcggaca tggtggtgac
    17521 taacgagaaa catcgtgata tttgcgagaa gaagtggaat atcccgagtg gcaccattcc
    17581 ggcgaaaatc ggtctgcacg cggtggcaca agaccgtgcg ctgaaagacg gcaagctgaa
    17641 tgtttactgg accatgtgta ccaacaacat gcaggccggg ccgaacatta atgaagagcg
    17701 tatgccaggc tggcgcgatc cgcgcaactt tatcatcgtt tccgatccgt atccgacagt
    17761 cagtgcgctg gcagcggact tgatcctgcc gaccgcaatg tgggtagaga aagagggggc
    17821 ttacggtaac gccgaacgcc gtactcaatt ctggcgtcag caggtacagg caccgggcga
    17881 agcgaaatcg gatctctggc agttagtcca gttctcccgc cgcttcaaaa ctgaagaagt
    17941 atggccggaa gagctgctgg cgaagaaacc ggaactgcgt ggcaaaacgc tgtacgaagt
    18001 tctgtatgcc accccggaag tgagcaaatt cccgctatcc gaactggcgg aagatcagct
    18061 gaacgatgaa tcccgcgagc tgggcttcta tctgcaaaaa gggctgttcg aagagtacgc
    18121 atggtttggt cgcggtcacg gtcacgatct cgcgccgttc gatgactacc acaaagcacg
    18181 cggtctgcgc tggccggtgg tgaacggtaa agaaacgcag tggcgttaca gcgaaggtaa
    18241 cgacccgtac gtgaaagcgg gcgaaggcta caagttctac ggtaaaccgg atggcaaagc
    18301 ggtgatcttc gcgctgccgt tcgaacctgc ggcggaagca ccggatgaag agtacgacct
    18361 gtggctctct accggacgcg tcctggagca ctggcatacc ggcagtatga ctcgccgtgt
    18421 accggaactg caccgcgcct tcccggaagc ggtcctgttt attcacccgc tggatgcgaa
    18481 agcgcgcgat ctgcgccgtg gcgacaaagt gaaagtggtt tctcgccgtg gcgaagtgat
    18541 ctcgattgtc gaaacgcgcg gtcgtaaccg tccgccgcag ggcctggtgt acatgccgtt
    18601 cttcgatgcc gcacagctgg ttaacaaact gacgctggat gcgaccgatc cgctctcgaa
    18661 agagacggac ttcaagaagt gcgcggtcaa actggagaag gtgtaagcca ttatgtcccg
    18721 gtcagcgaaa cctcaaaatg gtcgccgccg ctttctgcgc gatgtcgttc gcacagctgg
    18781 cgggctggct gccgtgggtg tggcgctggg gttacaacag caaaccgcac gcgcatctgg
    18841 cgtgcggttg cgcccgcccg gagccataaa cgagaacgcc tttgccagtg cctgtgtgcg
    18901 ttgcggtcag tgtgttcagg cttgccctta cgacacctta aaactggcga cgctggcctc
    18961 tggcctgtcg gcgggcacgc catattttgt cgcacgggat attccttgcg aaatgtgtga
    19021 ggacattccg tgcgccaaag tgtgcccgag cggtgcgctg gatcgtgaga tcgaatcgat
    19081 cgacgacgcg cgaatggggc tggcggtact ggtggaccag gaaaactgtc tcaactttca
    19141 ggggctgcgc tgcgatgttt gttatcgcga atgcccgaaa attgatgagg ccatcaccct
    19201 ggagctggag cgcaacacgc gtaccggtaa gcacgcccgc tttctgccga cggttcacag
    19261 cgacgcctgt accggttgcg gtaagtgcga aaaagtgtgc gtgctggaac aaccggcaat
    19321 caaggtgtta ccgctgtcac tagcgaaagg ggagttaggt caccattacc gcttcggctg
    19381 gctggagggg aacaatggca aatcgtaaac gtgacgccgg gcgcgaggcg ctggagaaaa
    19441 aaggctggtg gcgcagtcac cgttggttag tgttacgtcg cctttgtcag ttcttcgtgc
    19501 tggggatgtt tttgagcggt ccgtggtttg gtgtgtggat cttgcacggc aactacagca
    19561 gtagcctgtt attcgacacc gttccgctga ccgatccgct gatgacgctg caaagtcttg
    19621 ccagcggtca tctgccggcc tcggtggcgc tgaccggagc ggtgattatc accgtgctct
    19681 acgccctggc aggaaagcga ttattttgca gctgggtctg cccgctgaac ccgattaccg
    19741 acttagcgaa ctggttacgc aggcgctttg acctcaatca gtctgcgacg atcccacgcc
    19801 acattcgcta cgtgctgttg gtggtcattc tgctgggatc ggccttaacc ggcacgctca
    19861 tctgggaatg gatcaacccc gtttccctga tggggcgcag tctggtaatg ggattcggca
    19921 gcggcgcgct gcttattctc gcgctgtttt tatttgatct actggtcgtt gaacacggct
    19981 ggtgcgggca catttgtccg gttggcgcgc tgtatggcgt gctgggtagc aaaggtgtga
    20041 ttaccgttgc ggccaccgat cggcagaaat gtaaccgctg tatggattgt tttcatgttt
    20101 gcccggaacc gcatgtgcta cgtgccccgg tgctggatga acaaagcccg gtgcaggtca
    20161 ccagccgcga ttgcatgacg tgcggtcgct gcgtggatgt ctgttctgag gatgtattta
    20221 caataactac acgatggagt tcgggagcga aatcatgaaa agccatgacc tgaagaaagc
    20281 gctgtgtcaa tggacggcga tgctggccct ggtggtgagc ggcgcggttt gggcggctaa
    20341 tggcgtcgat tttagccagt cgccggaagt atccgggacg caggaagggg ccattcggat
    20401 gccaaaagag caggatcgga tgccgctgaa ctatgtgaat cagccgccaa tgatcccgca
    20461 tagcgttgaa ggttatcagg taacgaccaa taccaaccgc tgcttgcagt gccacggtgt
    20521 cgaaagctat cgcaccactg gcgcgccgcg tatcagtcct actcacttta tggacagcga
    20581 cggcaaagtg ggcgcagaag tggctccgcg tcgttatttc tgtctgcaat gtcacgtacc
    20641 gcaggccgat accgcgccaa tcgtggggaa tacctttacc ccatcaaaag gttacgggaa
    20701 ataagaggtt attatgggaa attctgaccg taagcctggt ctgattaagc gcctgtggaa
    20761 atggtggcgt acccccagcc gtctggcgct ggggacgctg ctgttgatcg gttttgttgg
    20821 cggcatcgtc ttctggggcg gctttaacac cgggatggaa aaagccaata ccgaagagtt
    20881 ctgcattagc tgccacgaaa tgcgtaacac ggtgtatcag gaatacatgg attccgtgca
    20941 ctacaacaac cgtagcggcg ttcgtgcgac ctgtccggat tgtcacgttc cgcacgagtt
    21001 tgtgccgaag atgatacgca agctcaaagc aagtaaagag ctgtatggta aaatttttgg
    21061 cgttattgac acgccgcaga aatttgaagc tcatcgcctg acgatggcac agaatgagtg
    21121 gcggcgcatg aaggacaata actcgcagga gtgccgtaac tgtcacaact tcgagtatat
    21181 ggatacaacc gcccagaaat cggttgccgc gaagatgcat gaccaggcgg tgaaagatgg
    21241 gcaaacctgt attgattgcc ataaagggat agcgcacaag ctgcccgata tgcgcgaagt
    21301 cgagccaggt ttttaacagg gttattgcgt gggtatgctt gaagccagag agttactttg
    21361 cgagcgggat gaacgaacct tatttagtgg cttgtcattt acgctgaacg caggagagtg
    21421 ggtacaaatc accggtagca acggcgcggg gaagacaacg cttctccgtt tgctgacggg
    21481 gttgtctcgc cctgatgcgg gtgacgttct ctggcaaggg cagcccttgc atcaggtacg
    21541 cgacagctac catcaaaacc tgttatggat agggcatcag ccggggatca aaacccggct
    21601 gacggcgtta gaaaatctgc acttttatca tcgcgatggc gataccgcac aatgtctgga
    21661 agccctggcg caggccgggc ttgccggatt cgaagatatt ccggtaaatc aactttctgc
    21721 cgggcaacaa cgccgcgtcg ctttagcgcg tctgtggctg acccgtgcca cgttatggat
    21781 cctcgacgag ccttttaccg cgattgacgt taacggcgtc gatcgtctga cccagcgtat
    21841 ggcgcagcat acggagcagg gggggattgt gattctgact acccaccagc cgctcaacgt
    21901 tgctgaaaat aaaattcgcc gcatttcact gacgcaaacg aggggcgaat aatgttctgg
    21961 cgcattttcc gtcttgagct gcgcgtagcg tttcgccata gcgccgaaat cgccaacccg
    22021 ctgtggttct tcctgattgt cattaccctt tttccgctca gtatcggtcc ggagccgcaa
    22081 ctgctggcgc gtattgcgcc gggcattatc tgggttgctg cgctgctttc atccttgctg
    22141 gcactggagc gactgttccg tgacgatttg caggacggca gtcttgaaca attgatgttg
    22201 ttgccgttac ccttgcccgc cgttgtgctg gcgaaggtga tggcgcactg gatggtaacc
    22261 ggtctgccgt tactcatcct ttcgcccctg gtagcaatgc tactgggaat ggatgtttat
    22321 ggctggcaag tgatggcgtt aacgctactg ctgggaaccc ctacgcttgg ctttcttggt
    22381 gcgccgggcg tggcgctgac agtgggactt aagcgcggtg gtgtgctgct cagcatactg
    22441 gtgttaccgc tgactatccc attactcatc tttgccaccg ccgcgatgga cgcggcttct
    22501 atgcatttgc ccgttgatgg gtatctggca attttaggcg cgttgctggc aggcaccgcg
    22561 acattaagtc cttttgcgac ggcggcagcg ttacgaatca gcattcaata acgctggatt
    22621 actgattttt gttcgagtct ggtatcgaaa ctatgtggaa aacactgcat caactggcga
    22681 tcccaccacg gctgtatcaa atctgtggct ggtttatacc gtggctggca attgccagtg
    22741 tggtcgtgct taccgtcggc tggatctggg gattcggttt tgctccggct gattatcagc
    22801 agggaaatag ctaccgcatt atctacctgc atgtgcctgc ggcgatctgg tcgatgggca
    22861 tttatgcatc aatggcggtg gcggcgttta ttggccttgt ctggcagatg aaaatggcca
    22921 acctggcggt ggcggcgatg gcccccattg gtgccgtgtt tacctttatt gccctggtta
    22981 ccggctctgc atggggaaaa ccgatgtggg gcacctggtg ggtatgggat gcacgcctga
    23041 cttctgaact ggtactgctg tttttgtatg tgggtgtgat tgccctgtgg cacgccttcg
    23101 acgaccgccg tctggcgggc cgtgcggcag gtattctggt gctgattggc gtggtgaatc
    23161 tgccgattat tcattactcc gtggagtggt ggaacaccct gcatcaggga tcaacgcgga
    23221 tgcaacaaag tatcgatccg gcgatgcgtt cgccgctgcg ctggtcgatt tttggcttcc
    23281 tgctgctgtc tgccacgctg acgctgatgc ggatgcgtaa tttgattttg ctgatggaaa
    23341 aacgccgtcc gtgggtgagt gaactgatac tgaaaagagg ccgtaaatga cccctgcatt
    23401 tgcttcctgg aatgaatttt tcgcaatggg cggttacgcc ttttttgtct ggctggcggt
    23461 ggtgatgacc gttattccgc tggtgatttt ggtcgtgcac tcggtgatgc agcatcgcgc
    23521 aattctgcgt ggcgtggcgc aacagcgggc gcgtgaggcg cgtttacgtg ctgcgcaaca
    23581 gcaggaggct gcatgaatat tcgccgtaaa aaccgcttgt ggattgcctg tgccgtgctg
    23641 gcggggctgg cgctgactat cggtctggtg ctatatgcgc tgcgctcgaa tatcgatctc
    23701 ttttatacgc cgggggagat tctctacggc aagcgtgaaa ctcagcagat gccggaagtc
    23761 ggtcagcgtc tgcgcgttgg cgggatggtg atgccgggga gtgtgcagcg cgatcccaat
    23821 tcgctaaaag taactttcac catttacgac gctgaaggtt cagtagatgt ctcttacgaa
    23881 ggcattttgc cggatctgtt ccgtgaaggg cagggcgttg tggtgcaggg cgagctggaa
    23941 aaaggcaatc atatcctcgc gaaagaagtg ctggcgaaac acgatgaaaa ctacacgccg
    24001 ccagaagttg agaaagcgat ggaagctaac caccgtcgcc cggcgagtgt ttataaggac
    24061 ccagcatcat gatgccagaa attggtaacg ggctgctgtg tctggcgcta ggaattgcgc
    24121 tgctgctgtc tgtgtatccg ctatggggcg tggcgcgcgg ggacgcgcgc atgatggcgt
    24181 cttcccgctt gtttgcctgg ctgctgttta tgtctgtggc tggcgcattt ctggtgctgg
    24241 tcaatgcttt cgtggtcaac gactttaccg tcacctatgt tgccagcaac tccaataccc
    24301 agcttccggt gtggtatcgc gtggcggcta cctggggcgc gcatgaaggc tcgctcctgc
    24361 tgtgggtgct actgatgagc ggctggacct ttgcggtagc gatttttagt cagcgtattc
    24421 cgctggatat tgtggcccgc gtactggcga taatggggat ggtcagtgtc ggctttttgc
    24481 tgttcattct ctttacctct aacccgttct cacgcacgct gccgaatttc ccgattgaag
    24541 ggcgcgatct taacccgctg ttgcaggatc cggggctgat cttccatccg cctctgctct
    24601 atatggggta cgtcggtttc tcggtggcgt ttgctttcgc cattgcttct ttgttgagtg
    24661 ggcgtctgga cagcacctat gcgcgtttta cccgcccgtg gacgctggcg gcgtggatct
    24721 tcttgacgct cggcattgtg ctcggttccg catgggccta ttacgaactc ggctggggcg
    24781 gctggtggtt ctgggatccg gtagaaaacg cctcgtttat gccgtggctg gtggggactg
    24841 cgctgatgca ctcactggcg gtcactgaac aacgcgccag cttcaaagcg tggacattac
    24901 tgctggcaat cagtgccttc tcgttgtgtc tgttggggac tttcctcgtg cgttccggcg
    24961 tgctggtatc ggtacacgcg tttgcgtctg atccggcgcg cggtatgttt atcctcgcct
    25021 ttatggtgtt ggtgattggc ggttcgctgc tgctgtttgc cgcgcgtgga cacaaagttc
    25081 gctcacgcgt aaacaatgcg ctgtggtcgc gggaatctct gctgttagcg aacaacgttt
    25141 tgctggtcgc cgcgatgctg gtggtattac tggggacgct gctgccgctg gtgcacaagc
    25201 aactgggact gggcagtatt tcgattggcg aaccgttctt caacaccatg tttacctggc
    25261 tgatggtgcc gtttgcgctg ctgcttggtg tcggtcctct ggtgcgctgg gggcgcgatc
    25321 gcccacgtaa aatccgcaat ttattgatta tcgccttcat ctcaacgctg gtgctgtcgc
    25381 tgctgctgcc gtggctgttc gaaagcaaag ttgtggcgat gacggtgctc ggcctggcaa
    25441 tggcctgctg gattgcgatg ctggcaattg cggaagctgc gctgcgtatt tcacgtggca
    25501 cgaaaaccac cttcagttat tggggaatgg tggcggctca cctggggctg gcagtgacaa
    25561 ttgttggtat tgcctttagc cagaactata gcgttgagcg tgatgtgcgc atgaagtccg
    25621 gcgatagcgt cgatattcat gaatatcgct tcaccttccg tgatgtcaaa gaggtgactg
    25681 gtccgaactg gcgtggcggt gtggcgacta tcggcgtaac gcgaaatggc aagccggaaa
    25741 cagtgctgta tgcggaaaaa cgttattaca acactgccgg gtcgatgatg accgaagcgg
    25801 caattgacgg cggcatcacg cgtgacctgt acgcggccct cggtgaagag ctggaaaacg
    25861 gcgcgtg
//
LOCUS       DAZKGF010000063        25630 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZKGF010000063 DAZKGF010000000
VERSION     DAZKGF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.063"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(329..2371)
                     /locus_tag="ACHOQV_002397"
     CDS             complement(329..2371)
                     /locus_tag="ACHOQV_002397"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9630763.1"
                     /translation="MQLFLCEKPSQAKDIARELALSKRGDGYIGDDNIAVTWAIGHLL
                     ELAEPEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLKRASEVVIATD
                     ADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQTALLSEAGKGR
                     ARADWLTGMNLTRLYTLKARERGISGVLSVGRVQTPTLAMVVRRDQEIATFVPVSWWQ
                     VIVWLEKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSAVVQDVVQKRE
                     KTPPPLCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKATTYPRTDCGYLPASMHE
                     ESSEVLEAVGRSDPSVLPQLAELESGRVSRIWNDKKITAHHAIIPTRQVFDLSSLNGD
                     ELKVYQLIRQHYLAQFLPEQEADVTEATFVIGGQLFRSRGRVNVIIGWKQLFPPENHA
                     DEEKPDSDESGNILPPLIKGELCALDGAEQKELKTVPPRPFTEGSLITAMKNASSGVT
                     DPALKKILRENAGLGTEATRAGILETLFARNYIERAGKHIRSTQLGRELVAALPDSLT
                     NPGMTALWEQALDDVARGKISLEAFMEKQHQWIRHLVLSGRNQTVNLSVESGPSCPLC
                     NGPTVKRKGKSGFFWGCSRYPDCRGIAGEQGNSRGRKRKINFSHKQKSGDVGDKLN"
     gene            complement(2374..3078)
                     /locus_tag="ACHOQV_002398"
     CDS             complement(2374..3078)
                     /locus_tag="ACHOQV_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFP9630764.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQKE
                     EKQEKRPDIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETIEKHLAWL
                     DGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVFQVFHYGL
                     ISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRMGEPDPDV
                     MSGKKRSVYSPPVKAG"
     gene            3249..3695
                     /locus_tag="ACHOQV_002399"
     CDS             3249..3695
                     /locus_tag="ACHOQV_002399"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="saposin"
                     /protein_id="HFP9630765.1"
                     /translation="MVIFYTLVPLAPLAIFCTLVPLAPLAIFCTLVPLAPLVIFYTLV
                     PLAPLVIFYTMVPLAPLAIFCTLVPLAPLAIFCTLVPLAPLVIFCTLVPLAPLVIFYT
                     LVPLAPLVIFYTLVPLAPLAIFCTLVPLAPLTIFCTLLPLATPLAF"
     gene            complement(3692..4171)
                     /locus_tag="ACHOQV_002400"
     CDS             complement(3692..4171)
                     /locus_tag="ACHOQV_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630766.1"
                     /translation="MQKYKQESLSAAMKESGLLIAKICACVIAGVVVIWGLVTGTETL
                     LTALKTMHEQYDNSMCYIVCATGWFFLVLSNWLWITYSLYKARTGSTVGGGLQFMLLL
                     SLLAECFSFFVIFWSLSHLDITDIQLLVMGITTLMLFLSLNVKVVVIINHVASGNSD"
     gene            complement(4283..5527)
                     /locus_tag="ACHOQV_002401"
     CDS             complement(4283..5527)
                     /locus_tag="ACHOQV_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFP9630767.1"
                     /translation="MTLPVDSLIAYTLEKMQERIVQKGRQQDGKIQSGLLFTGNVHDA
                     IPRRLLLDTRLSPLDKMAWMMIRLYAQNNEGAVFPTYDELQLQLASPGKGKASRETVS
                     RVLLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPVTCRDAEILDPRYLDMVADACM
                     SRNRTISQTARDILCEIKNDPGMRHYHSHISQLEARIGSAQTPGQMAARQRVTEDRMQ
                     PGSETEPGVETQSSVLKMLSSDSELSEKQGQKILSSDSEPSEKSEGYHRVRKPNYYVR
                     NITHSVIKNTYVLPEALTGQIREEEAIMLTSQLQALPEELAQGVLESLSGMLGSHSLQ
                     NPVGWLLAVLKRAREGNFYPPKQKQRVVVPASVLAGERGRATAQSVSSGKRTEPISAE
                     RLSGIVAGLRASLRRCSAMRPE"
     gene            complement(5545..6012)
                     /locus_tag="ACHOQV_002402"
     CDS             complement(5545..6012)
                     /locus_tag="ACHOQV_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630768.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGVLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
     gene            complement(6016..6507)
                     /locus_tag="ACHOQV_002403"
     CDS             complement(6016..6507)
                     /locus_tag="ACHOQV_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFP9630769.1"
                     /translation="MQISPVTLRVAKAFISRHHRHNKPPVGHKFSIGLRNDAGELTGV
                     ATAGRPVARHFDDGLTLEVNRTCTTGERNANSALYGAVWRAAKDIGYRRCITYTRADE
                     SGASLRAAGFVRVKELPSGKSWAESSVALKSTRDPVGSGGVPRVLREIRRMSTTVIHI
                     KGE"
     gene            complement(6510..6908)
                     /locus_tag="ACHOQV_002404"
     CDS             complement(6510..6908)
                     /locus_tag="ACHOQV_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630770.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VASCIIEDGGMCVDGVGEYVGHSLPGGTYQLYTASPVPVVPEGLVKAVRFYEQVKREN
                     PPVETGAWKDAVDWVLKEACQAVNIRIKGE"
     gene            complement(6912..7199)
                     /locus_tag="ACHOQV_002405"
     CDS             complement(6912..7199)
                     /locus_tag="ACHOQV_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630771.1"
                     /translation="MATLQELIDLTPEQEKAWKRLVKAVKDFRAAGGKFYSVLDTLSA
                     YNGEHVASIDNDKGYHTARVYMPGIDAPGLTSWADDWHGITLKDGVEVDDD"
     gene            complement(7202..7351)
                     /locus_tag="ACHOQV_002406"
     CDS             complement(7202..7351)
                     /locus_tag="ACHOQV_002406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630772.1"
                     /translation="MAEINFQVLREAEQVKRENQPVEAGAWKDAVEWVLQEDCQAVNI
                     RIKGE"
     gene            complement(7366..7974)
                     /locus_tag="ACHOQV_002407"
     CDS             complement(7366..7974)
                     /locus_tag="ACHOQV_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFP9630773.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            complement(7971..8696)
                     /locus_tag="ACHOQV_002408"
     CDS             complement(7971..8696)
                     /locus_tag="ACHOQV_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFP9630774.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYYDWRAVPGGRY
                     RRCVTFYGFSERPQVAAYAFDVLCRQLREATDAYLRTQSRRLKLSTRRARAAQFRDGW
                     VQGIRQVVMPFSVTEEESRLMRLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNA
                     SLHHGVGESARQVVPGLMIREAS"
     gene            complement(8689..10422)
                     /locus_tag="ACHOQV_002409"
     CDS             complement(8689..10422)
                     /locus_tag="ACHOQV_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFP9630775.1"
                     /translation="MAHKPLDLGAAIMQPGRQTTGAGNVVAMGETPMVLTLDQLRPNP
                     DNPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDGGNTRYQILTELWK
                     ETGDPRFYRIHVLFKPWPGRLQCVIGHLAENELRGELTFIEKAQGIHKARLIYEESLQ
                     RQVTIRELATLLTDEGLPVSHTSISRMEHALKYLYPWIPDLMESGLGRPQVTALLALR
                     QDAERVWGQFAVAADTDAEFDRVFGESCRKFNSPELWSLEMFRDELIGDLLQALLHPS
                     LDYDRWLLELDPKERNRRHHFGEPEPVVVPPSVRGSSTVQPGAPVSGIRSEVPECQPD
                     TDTEAMPESTVSIFPGTERKQHGITRPLKAVSDTDTERTEKCSRPSQEESAGRGDSGA
                     SSAENALRPEIQPDMYGGKPVFSGDVMEDGAAGAVLASVMTGDTAPGTTDALPASVTH
                     FAVGRDDIEHLQNEAFRLAWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVVAFL
                     AGLAGDAPRTVLPVSLKDLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRALQRSVL
                     PDDDEGDENDE"
     gene            complement(10422..11789)
                     /gene="dnaB-PI"
                     /locus_tag="ACHOQV_002410"
     CDS             complement(10422..11789)
                     /gene="dnaB-PI"
                     /locus_tag="ACHOQV_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPI-7-type island replicative DNA helicase"
                     /protein_id="HFP9630776.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLAL
                     SMCLSAALRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDEDWLRVSS
                     AVGMALGDGGMGSLGERIIIDDCCEQTPSSLRAGARRYTRLYGKPSLIMVDYLQLIRA
                     PELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALE
                     QDADLIMFIYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSENDG
                     PGGYA"
     gene            complement(12137..13039)
                     /locus_tag="ACHOQV_002411"
     CDS             complement(12137..13039)
                     /locus_tag="ACHOQV_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630777.1"
                     /translation="MSSSVKYSPVPAVLTRPESLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNRERMCEWFSQVWLPLHLAALEGDE
                     NAFFSAEVNMVRRELQRVPGDSVNCMCWASLAALAFDAEERVELQKGNNENIATYDES
                     IATYAFRAACTVSRCLSRNASGGIPRSYPEWKAILFPRDGADIFPDGWLENMTRRAER
                     VFTSAKRQCGMLSASQAALFSQQTRQCASEQCTGRIQAGRHHSGSHPDVSVYAERCAQ
                     ETSLSPVHPTRQCPVQSTDSAETE"
     gene            complement(13024..13647)
                     /locus_tag="ACHOQV_002412"
     CDS             complement(13024..13647)
                     /locus_tag="ACHOQV_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630778.1"
                     /translation="MGRKLIWRAVASAPGHEEVHVFVDASDRAEAKKHLYQRLAEEWR
                     CSVNRITFHNMWNENELREMSTGPRKPEGLPLLESGGGNGGVFYDNEPLLLIASWRLR
                     ASLETALIVLQKWTVCVGCGYSGVRDGAGKNAPVWVKVNPESGLGLCSGCDSFLLQER
                     QRRQRHDQLFSCGLSCKQRKTEGAVYLRGSDAPGGLRDMEVKVCLPQ"
     gene            complement(13632..14519)
                     /locus_tag="ACHOQV_002413"
     CDS             complement(13632..14519)
                     /locus_tag="ACHOQV_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9630779.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFQTYDVIVVDSQGARSVMLEMIVLSATESVVGMVNPVLPDVR
                     EFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTALARQTLAELTGIINAGRYSKV
                     LPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSARCSDSALVTMHSLACELLPEW
                     RAFFDALLENGGKSLWAEN"
     gene            14835..16016
                     /locus_tag="ACHOQV_002414"
     CDS             14835..16016
                     /locus_tag="ACHOQV_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9630780.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYIDGRREMMQWYADYIDSLSEQ
                     T"
     gene            16292..16921
                     /locus_tag="ACHOQV_002415"
     CDS             16292..16921
                     /locus_tag="ACHOQV_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9630781.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWPALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            16921..18462
                     /locus_tag="ACHOQV_002416"
     CDS             16921..18462
                     /locus_tag="ACHOQV_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP9630782.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALLILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPAILAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNVCKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            18535..19851
                     /gene="uhpC"
                     /locus_tag="ACHOQV_002417"
     CDS             18535..19851
                     /gene="uhpC"
                     /locus_tag="ACHOQV_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP9630783.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLNANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWSGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            19848..20879
                     /locus_tag="ACHOQV_002418"
     CDS             19848..20879
                     /locus_tag="ACHOQV_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9630784.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            20948..23026
                     /locus_tag="ACHOQV_002419"
     CDS             20948..23026
                     /locus_tag="ACHOQV_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9630785.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFILLPSWSLDYGLLESTS
                     DEILEAYGWSHFNISWLWYLLPSLLLVRPFREATREQRSRHYFDASWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMNVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYITEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCVQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILTVWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPLYLAGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            23038..24084
                     /gene="fbpC"
                     /locus_tag="ACHOQV_002420"
     CDS             23038..24084
                     /gene="fbpC"
                     /locus_tag="ACHOQV_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFP9630786.1"
                     /translation="MSQKNFVELRNVTKRFGSNRVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKDALTMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTAGVR
                     PEAITLNNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            24283..25630
                     /locus_tag="ACHOQV_002421"
                     /pseudo
     CDS             24283..25630
                     /locus_tag="ACHOQV_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         5827 a         7280 c         6297 g         6226 t
ORIGIN      
        1 gtagccaccc gaaggcgctc ctctcaggcc acttgtggag cattggtggt cgccggagcg
       61 acgttatgtg cccggacaga ctgaaatcac tgatggaaaa agtcaacagc taaccaaaaa
      121 caaaaaagca ccggcaagcc ggtgcttcag ggggatacca aatcccggtg ttgattctca
      181 tcttctccgg agagagcgac tcccggcacg gttttcacgg agtgcatgtg ccttgtaacc
      241 ccgccgtgtg cataacgggt taccggacct ctcagggctg tcagccccac tgtggtcaac
      301 agggtgatac cagaactcgc ccgcaatttt aattcagttt gtctccgaca tcaccagatt
      361 tttgtttatg agaaaaatta atttttcttt tcctgcccct gctgtttccc tgctcaccag
      421 caattcccct gcagtcggga taacgggagc atccccagaa aaaaccgctt tttcccttcc
      481 gtttcaccgt cggaccgtta cagagtggac atgaaggacc tgattccact gacagattaa
      541 ccgtctggtt acgtccggat aataccaggt gcctgatcca ctgatgctgt ttctccataa
      601 acgcctccag tgagattttc ccccgggcaa catcatccag cgcctgttcc cataaagccg
      661 tcatacccgg atttgtcaga gagtccggca atgccgccac cagttctctt cccagttgcg
      721 tggaacggat atgttttccc gctctctcaa tatagttgcg cgcaaataac gtttccagga
      781 tccccgcgcg tgtggcttcc gttcccagtc ccgcattctc cctcaggatc tttttcagcg
      841 ccggatccgt tacaccagac gaagcattct tcatcgcagt aataagactg ccttcagtaa
      901 aaggacgggg aggtacggtt ttcagctcct tctgttcagc accatccaga gcacagagtt
      961 ctccctttat caaagggggc aatatgtttc cactttcatc actgtcaggc ttttcttcat
     1021 ctgcatgatt ttccggtgga aataactgtt tccacccgat aatcacattc actcttccgc
     1081 gggaacggaa taactgcccc ccaatcacaa atgttgcctc agtaacatca gcttcctgct
     1141 ccggcagaaa ctgtgccaga tagtgctgac gaatcagttg atataccttc agttcatcac
     1201 cgttcagtga gctgaggtca aaaacctgcc gggtcggaat aatggcatgg tgagccgtaa
     1261 tttttttatc attccagata cgggataccc gtcctgattc cagttcagcc agttgtggaa
     1321 gcacggacgg atctgaacgc ccgacagcct caagcacttc agaactctcc tcatgcatcg
     1381 aggcaggcag ataaccacag tctgtcctgg gataagttgt tgctttgtga gtctcataaa
     1441 gggactgtgc gatatccaga acggtctgag cccccatgcc aaacttccgg gaacaggctt
     1501 cctgcaacgt tcccagcgaa aaacacaaag gtggcggtgt tttctccctc ttctgtacaa
     1561 catcctgtac aacagcagag gactgctgac ggcacagacc gagaacagca ccggccgcct
     1621 gcacattcac acaacgtccc tcatcatcgc agtactgctc agctgccacc cagttactgc
     1681 ggaaacggag cccctccttt tccagccaga caatcacctg ccaccaggaa accggcacaa
     1741 atgtggcgat ctcctgatcc cgtctgacca ccattgccag cgtgggtgtc tgcacccggc
     1801 ccactgacaa aaccccagaa attccacgtt cccgtgcctt cagggtatac aaccgggtca
     1861 gattcatccc tgtcagccag tcagcccggg cacgcccctt accggcttca ctgagaagcg
     1921 cagtctgttc gcccgggaga atattattca gcgccttcct gatactggcc tcatccagtg
     1981 cagataacca cagacgcctg acacgccctc tgtactgaca gtactccagc aactctctgg
     2041 ctatcacttc tccttcacga tcggcatctg tggcaataac cacttcagaa gcgcgtttta
     2101 acagcccccg gatcacaata agttgttttt tcaccttcgg cttcaccacc atctgccatt
     2161 gttccggtat cagcggcaac acatcagaac gccatgggga accgaactgt tcaccgtaag
     2221 cttccggctc agccagttcc agcaggtgtc ctatcgccca ggtgacagca atattgtcat
     2281 ctccgatgta tccgtcgccc cgcttactga gcgccagttc acgggcgata tctttcgcct
     2341 gagaaggttt ttcacacaga aagagttgca tactcacccc gctttaaccg gtggtgaata
     2401 aacagaacgt ttcttaccgc tcatcacgtc aggatccggc tcccccatgc ggttaaccgc
     2461 ctcctttcca cgggctgtgc cggacaaaat atcagcacgt gtaacactga ctgatttata
     2521 attttgaata atcccgtaaa tcctgcgtac cgcataccct ccctgattca acaactcatc
     2581 ccgttttgtg cgggaaataa gtccgtaatg aaatacctga aataccttca tgaccagtcg
     2641 atcatatccg atcagaagcc atacacatcg ataaccgacc ggagaactac tgtacacgcc
     2701 aatatttacc ggcgaagagg atttaatatc agaaagggag atctgtcccg gcaggttttt
     2761 aaggatcccg tcaagccacg caagatgctt ttcaattgtt tcagttgcct tcgtcagggc
     2821 attttccagc ctgactaaca tcatatctgc ataaggatta tccctggctg aatctctggt
     2881 tgcctgaccc gcaaacgcaa tagccctggg catactgatg atgtcaggcc gcttttcctg
     2941 tttttcttcc ttctgattat tcctgcgtcc ctcccagaga cggatcgcat atcgggtatg
     3001 aagagcaata gtcagcgatg actgcagagc accaacttgt tctgattttc tgacttctcc
     3061 ggttttctcc tggggcattg tgtttactcc gaatgatttc atgaccagaa gatagtccgc
     3121 aggttgcgta ttaccgacaa caaaaaaaca aatcaggcag aatatacatt taacgccgct
     3181 tattttttaa tcgctgttgc cactggcacc attgggttat tttttatact atggtgccac
     3241 tggcaccatt ggttattttt tatactctgg tgccactggc accattggct attttttgta
     3301 ctctggtgcc actggcacca ttggctattt tttgtactct ggtgccactg gcaccattgg
     3361 ttatttttta tactctggtg ccactggcac cattggttat tttttataca atggtgccac
     3421 tggcaccatt ggctattttt tgtactctgg taccactggc accattggct attttttgta
     3481 ctctggtgcc actggcacca ttggttattt tttgtactct ggtgccactg gcaccattgg
     3541 ttatttttta tactctggtg ccactggcac cattggttat tttttatact ctggtgccac
     3601 tggcaccatt ggctattttt tgtactctgg taccactggc accattgact attttctgta
     3661 ctctgttgcc actggcaaca ccgctcgctt tttaatcact gttgccactg gcaacatggt
     3721 taatgatgac tactacctta acgttcagag acaaaaacag cattaatgtt gttatcccca
     3781 tcacgagcaa ctgaatgtcg gttatatcca gatgagacaa tgaccagaaa atcacaaaaa
     3841 atgaaaagca ttctgccagc agactcagga ggagcataaa ttgcaggccc cctccgactg
     3901 tactgcctgt acgcgcttta taaagactgt atgtgatcca cagccagtta cttaacacca
     3961 ggaaaaacca ccctgtggca catacgatat aacacatact gttatcgtac tgttcgtgca
     4021 ttgttttcag cgcagtgagc agggtctctg tgcctgtcac cagtccccag ataaccacca
     4081 ctccggcaat cacgcaggca caaattttag cgatcaacag gccactttct ttcatggctg
     4141 cactcagact ttcctgtttg tatttctgca tgctatttac caccttgttt cacctgacca
     4201 gttcccgatt ctgttttctt tttaaccagt cgctggcggc actggttgat ttttattccc
     4261 gcgaacatag cccgtatcgc aatcactcag gtctcatcgc agaacatctg cgcagcgagg
     4321 cccgtaatcc agccacaatg ccactcagac gttctgctga aatcggttcc gtacgtttcc
     4381 ccgatgaaac tgattgcgca gtagctcgcc ccctttctcc tgccagtacg gaggctggca
     4441 caacaacacg ttgtttctgc ttcggcggat aaaaatttcc ctcccgggct cgcttcagca
     4501 ctgcaagcaa ccagcccaca ggattctgca gcgagtggct gcccagcatc cctgacagac
     4561 tttccagaac cccctgcgcc agttcttccg gcagcgcctg caactgactg gtcagcataa
     4621 tggcctcctc ctccctgatt tgccctgtca gcgcttcagg caatacgtac gtgtttttaa
     4681 tcacactgtg tgtgatatta cgtacgtaat agttcggttt ccgaactctg tgataacctt
     4741 ctgatttttc tgatggttcg gaatccgaac tcagtatttt ttgcccctgt ttttcactga
     4801 gttcggaatc cgaactcagc attttcaata ccgaactctg tgtttctacc cccggttcgg
     4861 tttccgaacc cggctgcatt ctgtcctcag tcacccgctg acgggccgcc atctgtcccg
     4921 gtgtctgggc actccctatc cgggcttcca gttgactgat atggctgtga taatgacgca
     4981 ttcccggatc attttttatt tcgcacagaa tatcccgggc cgtctgactt atggtccggt
     5041 tccggctcat acaggcatct gccaccatat ccagataacg tggatccagt atttcagcat
     5101 cccggcacgt caccggttca tcgtgctggg cataaatatt gccccgtacc cgccctttct
     5161 catcacgcac ccgtttgcac agactcagcc agcccgtgat ccgcagcatc agcagcaccc
     5221 ggctgacagt ctccctggag gcttttcctt tccccgggga agcaagctgc agttgcagtt
     5281 cgtcatatgt ggggaaaact gcaccttcat tattctgtgc atacagccga atcatcatcc
     5341 acgccatctt atccagcgga gaaagtcgcg tgtccagaag cagtcgacgc ggaatggcat
     5401 catgcacatt tccggtaaac agcagtccgc tttgtatttt tccgtcctgt tgtcggcctt
     5461 tctgcactat ccgctcttgc attttttcca gcgtgtacgc aatcaggctg tctacgggta
     5521 atgtcattac acttttcctt tttgttatac gtcagacatc cgtctgctgg taacgggtat
     5581 atcgcccata ttcaccagac agttacgccg gagatatgcc agcgcttcat caggaacggt
     5641 acattctggt ctggcttttt tcaggttact gattaatcgt ttcatcagca ttactggttc
     5701 atgtgcacgg atccaccgtc cccctgtttg atcttccaga cagcgaatta acatctcagc
     5761 tggcaaaaga accccgtggt tctcacactc ctttttcttc tctgccgcag ttaatttctg
     5821 ttgcaattca acaattgctg ccagcgcaga tttaatcgtt tcactcgtca cccgacatgg
     5881 ataagcacga ttcctgcctt ctttcagaag cactgcataa ccatcagagc ggtagtgata
     5941 acagtttgct cccaaccaga tttcgtaagt atcgccgcgc gaggagatac ccgttctctc
     6001 tgcaatagcc atatctcatt cccctttgat gtgaatgaca gtagtgctca ttctcctgat
     6061 ttcccggagc acacgaggaa caccaccgct tccaaccgga tcgcgtgtac tcttcagggc
     6121 gacacttgat tccgcccagc tttttcctga aggaagttct ttcacacgaa caaaaccagc
     6181 tgcccgaaga gaggccccgg attcatcagc ccgggtgtat gtaatgcaac ggcgatagcc
     6241 tatatctttt gccgccctcc agacagcgcc ataaagtgcg ctgttagcgt tacgttcacc
     6301 agtagtacag gtacgattga cctccagtgt cagcccatcg tcaaagtggc gtgcaacagg
     6361 tctgccggct gtcgccacac ctgtcaattc acccgcatca tttctcagac caatactgaa
     6421 tttatgcccc acaggaggtt tattgtgtcg gtgatgtctg gatataaacg ccttcgcaac
     6481 acgaagagtg actggtgaaa tctgcacgct cactctcctt tgatgcgaat atttacagcc
     6541 tggcaagcct ctttgagcac ccagtcaacc gcatctttcc atgccccagt ttcaactggc
     6601 ggattctcac gctttacctg ttcataaaag cgcactgctt taaccagtcc ttcaggcact
     6661 accggcacag gtgaagcggt gtatagctgg tacgttccac caggcagcga gtgacctaca
     6721 tactcaccga ccccgtcaac acacatgccc ccatcttcaa taatgcatga cgccacaggc
     6781 tctgcttcca gcgatgccag cgcgataagc gccagctcac gaatctcacc gccgtctata
     6841 tcgtcaatgt catcgcggcc agaaatgtta gccagccatt gcagtcgctc tttggtaatc
     6901 gtggtcatgt gttagtcatc atccacttca acgccatctt tcagcgtgat gccgtgccag
     6961 tcatcagccc aactggttag ccctggcgca tcaatgccag gcatatagac tcttgcagtg
     7021 tggtagccct tatcgttatc aatgctggca acgtgctcgc cgttgtatgc actcagcgtg
     7081 tccaggacgc tataaaactt tcctccggct gccctgaaat cctttacagc cttcacaagg
     7141 cgcttccacg ctttttcctg ttctggcgtc aggtcgatta attcctgcaa agtagccatg
     7201 ttcactctcc tttgatgcga atgtttacag cctggcaatc ctcctggagc acccactcaa
     7261 cggcatcttt ccacgctcca gcctcaaccg gctggttctc tcgcttcacc tgttcagcct
     7321 cccgcagtac ctgaaagtta atttcggcca tgtcatttgt ccttgtcatt ctgctttttc
     7381 ccggttcagt ttccggcgac tggccgatac atcattaaac cccgcagttc cctgatgcca
     7441 tcccctcact gcatgccata ccgccgtcag cgggatattc atttgctcag ctgccagcat
     7501 catgacatcc agtccgtccg cactttccac atctgtgatg ccgctcttct gccataaacg
     7561 ccacagctct gcactttcct catcactcag tgtgatgcca cgtcccgggc gaacatcaat
     7621 accggcaatg cgacggcgcg ccgccacatc cacactggaa agtccaaagt aaaacgccat
     7681 cagttcaatg gagcctccca gcgccagtgc ccggtcaatg cgctgcagac gtttctgctc
     7741 cgtccgggcc tgctgcagca tacgcagcag gttttcatga ttaatgtcca gccggataac
     7801 cgacacttca gagtttgaca ggtaatgaat ttcctcaatg gtcagtccct gcagcatctg
     7861 catttcctcc cgggccagcc ccagagattc gcagcgacgc aggtagccgc tcttgaggtc
     7921 catcaccagt tgcatcagca gaccgttggt tgcctgagac aagctgttat tcatgacgcc
     7981 tcccgtatca tcagacccgg caccacctgt cgggcactct cccccacacc atgatgcagt
     8041 gaggcatttt tcccggcctc atacccctgc cagcgggcca ggtcaccgcc gcgacaggct
     8101 ttcatctcac gggagtccac cgtcaccata tcctgctgct ccatccagag acgcatcagc
     8161 cggctttcct cttctgtcac actgaatggc atcaccacct gacgaatccc ctgaacccag
     8221 ccatcacgaa actgagccgc ccgggcacga cgggtgctca gtttcagtct ccggctctgt
     8281 gtacgaagat acgcatccgt tgcttccctt agctgacggc acagaacatc aaacgcataa
     8341 gccgccacct gaggacgttc actgaaaccg taaaacgtca cacaccggcg ataccggcct
     8401 ccgggcaccg cccgccagtc gtaatacgcc cggcagccaa atgtctgtgt caccaccatg
     8461 accagacgaa acatccacgc cggaactttt tcagcatcag aaggtgcccc ctgtgatgaa
     8521 gaggtctgca ccgaagaaag tccggcatcc tgctccgtga taccgtgttt cgccatcagc
     8581 tgttgcgccc ttgcaagcgc cagcccggcc tcatgggcat tgctgttatt acggctcagc
     8641 tccagcagtt tcctgacccg cctgatcaga cgttcctgat gcgacatatc actcatcatt
     8701 ctcatccccc tcatcgtcgt cgggtaatac actgcgctgc agggctcgca ggcggcgcag
     8761 cacactcaga agacgcagca gcttcacggc atggtcgtca tccagcagtg gccacgcttc
     8821 cggtgcactg ccccctgtaa gcaggtcttt cagactcacg gggaggactg tccgcggtgc
     8881 atcaccggcc agccccgcca gaaacgccac tacaggagaa catttctcca ctgctgcccc
     8941 gaaaccggca gacaggtcgc tttccctgtc catggcaatc tcttcagcac aaccggctga
     9001 ttcagccagt tcccaggcca gacggaacgc ttcattctgc agatgttcga tatcatcccg
     9061 ccccacagca aagtgcgtca ccgacgcggg aagagcatca gtggttcccg gagcagtatc
     9121 tcctgtcata actgatgcca gtactgctcc ggcagcacca tcttccataa catcaccact
     9181 gaagactggc ttaccaccgt acatatccgg ctgtatctca ggacgcaggg cattttcagc
     9241 agaagacgcc ccactgtccc ccctgcctgc gctctcttcc tgagaaggtc gactgcattt
     9301 ttcagtgcgc tctgtgtcag tatcagacac ggctttcagt ggccgggtaa taccatgttg
     9361 cttgcgctca gtaccgggga atatggagac tgtgctttcc ggcatcgcct ctgtatccgt
     9421 atccggttga cactccggta cttcagaccg gatcccggat accggtgccc cgggttgtac
     9481 cgtactgctg ccacggactg aaggaggcac caccaccggt tccggctcac caaaatgatg
     9541 acgacggttt cgctctttcg gatccagttc cagcaaccac cggtcatagt ccagggaggg
     9601 atgtagcagg gcctgcaaca aatctccgat aagctcatcg cggaacatct ccagtgacca
     9661 cagttccggc gaattaaact tccggcagct ttccccgaac acgcggtcaa attccgcatc
     9721 cgtatcggct gcaacagcaa actgccccca tacacgttct gcgtcctgac gaagtgcgag
     9781 gagtgctgta acctgtggac gccccagtcc tgactccatc aggtcgggga tccagggata
     9841 caggtatttc agggcatgct ccatccggct gatggaggta tggctgacag gcaacccttc
     9901 atcagtcaat aaggtcgcca gttcccgtat cgttacctga cgctgcagag attcctcata
     9961 aataagtcga gccttgtgaa ttccctgcgc cttctcaata aaagtcagtt cgccccgtag
    10021 ctcattttca gccagatgac caatcacaca ctggagtcga cccggccagg gtttaaacag
    10081 cacatgaata cggtaaaaac gcggatcccc tgtttctttc cagagttccg tcagtatctg
    10141 gtaacgggtg tttcccccgt cactgaagat atacacatcc ggctcactgt ccggcatacg
    10201 ggtgactttg ggcaccgtat ccagcccgcg ggcatgaatg gaatttttga tatcgtcata
    10261 acgcggattg cgcgtggtgc gcgggttatc cggattcgga cgtaactggt ccagcgtcag
    10321 aaccatgggc gtttccccca ttgccaccac attgccggca ccagtggtct gacgaccggg
    10381 ctgcataata gccgccccga gatccagtgg tttatgtgcc atcacgcata tcctcccggc
    10441 ccgtcattct cagagggcgt aaacagtgtg taacgaccgt cgaacctgac ccgtaccgta
    10501 cccgtgggcc cctgacgctg cttgctgaca atgatttctg cctccccctt tgccagtgag
    10561 tcggggttat agacctcgtc gcggtaaata aacataatca ggtcagcatc ctgctccagc
    10621 gccccggaat cacgcaggtc accgttattg gggcgtttat cagcacgctg ctccacctgc
    10681 cggttaagct gggagagggc cagcaccggg cagcccagct ccttccccag cgctttcagg
    10741 gaacgggaaa tttcagcaat ttcctgtgtg cggttttcca gctccggcgc acgaatcagt
    10801 tgcagataat ccaccataat cagggagggt ttaccgtaca ggcgtgtata acgacgggcg
    10861 cctgcacgca gtgatgaagg cgtctgctca cagcagtcat caattattat gcgctcaccc
    10921 agagagccca ttcctccgtc acccagcgcc ataccgaccg cagatgacac cctcagccag
    10981 tcctcatcat ccatgttgcc actgcgcaaa tgattcagtt ccacacgccc cagcatggaa
    11041 agcatgcgca gaatcagttg ctccttcggc atctcaatgc tgaataccag aacgctctct
    11101 tccgttcgcc gcagtgcggc gctgagacac atggatagcg ccagagaggt tttccccatt
    11161 gacggacggg cagccagcag aaccaggtca ccaggctgca ggccacaggt catttcatcc
    11221 agacagtcaa atcccgtggg ggtaccggta acaccaccgg aaccgagcac actttccagt
    11281 tgctgcagaa cggtatcaag cgcggatgaa agagaggctt ccgtgcgctc ccggagcgtc
    11341 ctgttctgcg caagcgcaaa cagacgccgc tctgcagcat ccagcacatc ccgtgacgtg
    11401 gcatttggtg ccgccacatc agcaatcagg gtgtttccgg tggcctgtaa ctgacgcaga
    11461 acgcttttct ccaccacaat gcctgcatag gccatgatgt tggccgcaga aggtgtgttc
    11521 ttgctgagct cggccagata agcaaaaccg cccagtacgt ccagcctgcc ctgatgttcc
    11581 aggtgctctg acagtgtaat caggtccagt ggctggccat tcaccgccag ttcagacatg
    11641 gcacggaaaa tggcccggtg ctgagccaca taaaaatcgt caggactcag catcagcacc
    11701 acttcatccc agcgttcatt ctccagcatc agcccaccca gaacggcctg ctctgcttcg
    11761 tatgcgcaga gaacagacat ttcagccatg gcgtaccctc cctctctgac tgccggaaac
    11821 ctggctacgt gaaaaaagga aaaagggtgc cggacataca gttgtcggga ataaaatccc
    11881 ggtaactgtc tgtacgtttt cagaccggtg agtttgccga ccccggtctg aaggtacgga
    11941 cagcattcgt tcattcataa caagttgttt taaaggaaaa agtttatgaa tacagaaaaa
    12001 caactgccgt ccggcattga atctgaagtg aaatacaccc tgcagttctg gctgctgagt
    12061 aacggaaagg tcatgacgga aaaagacggg ctctgtaacc cctttccccc gaactgtatt
    12121 gaatcggctc atccttttac tccgtttcag cagaatcagt ggattgaact ggacactgtc
    12181 gggtcggatg gaccggtgaa agcgacgttt cttgtgcaca gcgttctgca taaactgaaa
    12241 cgtcagggtg gctccctgaa tgatgccggc cggcctgaat acggccggtg cattgctcac
    12301 tggcacactg acgtgtttgt tgagaaaaaa gcgctgcctg actggctgag agcattccac
    12361 actgacgttt cgcactcgta aaaacacgtt cagcccgacg ggtcatattt tccagccacc
    12421 cgtcggggaa aatgtcagca ccatcacggg gaaacaaaat cgctttccat tcagggtatg
    12481 agcgcggaat acctccggaa gcattccggc tcagacagcg actcacagtg caggcagcac
    12541 ggaatgcata tgttgcgatg ctctcatcat atgttgcgat gttctcatta ttgcctttct
    12601 gcagttccac ccgctcttca gcatcaaatg ccagtgcagc aaggcttgcc cagcacatgc
    12661 agttcactga atcccccgga acgcgctgca gctcccggcg caccatgttg acctcagcac
    12721 tgaaaaaagc gttctcatcg ccttcaaggg cagccagatg cagcggaagc cagacctgac
    12781 tgaaccattc gcacatccgt tcccggttac tgacggtcat cactgtctcc gggcgggaac
    12841 cgtcctgaaa aacatgtccg gtgcgtggag aactgtatga aacataacca ccgtgcaccg
    12901 caatgaccgg acgacctgac gccggaagcc ccacacgaca cccactggca atccgggtca
    12961 tcagcaggtt cagttcagcc agcgattcag ggcgggttaa gaccgccgga accggagaat
    13021 atttcactga ggaagacata ctttcacctc catatctctc agcccgccag gtgcgtcact
    13081 tccccgcaga tataccgccc cctccgtctt tcgctgtttg caggaaagac cacatgaaaa
    13141 aagctggtca tgccgctgac gacgctggcg ttcctgcaaa agaaaggagt cacaaccgga
    13201 acacagcccc agaccactct caggattcac cttcacccag accggagcat tcttaccggc
    13261 accatcccgt accccggaat aaccacagcc cacgcacacc gtccacttct gcagtacgat
    13321 gagtgcagtt tcgagtgatg cgcggagacg ccaggaggca ataaggagca acggctcatt
    13381 gtcataaaaa accccgccgt taccccctcc ggattcaagc agcgggagtc cttcaggctt
    13441 ccggggacct gttgacattt cccgtaattc gttttcattc cacatattgt ggaaagttat
    13501 ccggttcact gaacaacgcc attcctccgc cagacgctga tacaaatgct ttttcgcttc
    13561 tgcgcggtca gatgcatcaa caaacacatg cacttcctcg tgtccgggtg ctgaagccac
    13621 ggcacgccat atcagttttc tgcccacagg cttttccctc cgttttccag cagagcatca
    13681 aaaaacgcac gccactccgg caacagttca caggccagac tgtgcatggt gaccagcgca
    13741 gaatcgctgc aacgggcaga cactttttcc agacgatgca ccggctgacc ggctgcgtgc
    13801 ccctgtttat aaatattcag gtcgtaaatc tgtgttgaaa gaagggtgac cgcaccttca
    13861 ggcagcactt tgctgtagcg accggcgttg ataatgcctg tcagctctgc cagtgtctgt
    13921 ctggccagcg ccgtataatc catgcagtta accagggtgc ggactttcgg caggggaata
    13981 cccagttcac ggtagggcat aaggttttcc atgacattga ccgtgccgcg gatgaactca
    14041 cgcacatccg gcagaaccgg attcaccatc cccaccacag actccgtggc ggacagcaca
    14101 atcatttcca gcatgacaga acgtgcgccc tgtgaatcca ccacaatgac gtcataggtc
    14161 tgaaaaagcg gatgctgaag cacattacgc agacgaaggc gaccatccgg cgcatgaagc
    14221 atggccgttt tcagttgctc atgggggtcg ttggagacga tgaggtcaag ccccctgaca
    14281 gccgtacggg aaatgatgtt gtcaggatga ctgaggtcag ccgtgcgcat cagtaattca
    14341 aaaagaccgg ccggcgcttc atacgtcagc ggaaagatat tgcttgccgt gggctgtgca
    14401 tggtctccgt ctatcagcag agtacggagg ccggcatctg ccagaaaacc ggcaagatta
    14461 gcagactggg ttgattttcc ttcgccacct ttagtggata taaccggtaa aattttcata
    14521 tagcacactc tttaaagtat gcaaccaatc acttacacta cggaaaccag ccagaatatt
    14581 cacttcacta cctgaatact cgcaacacgg ggattgaaaa tccccgtgtc cttggttcga
    14641 ttccgagtcc gggcaccact ttcacttcca gattcatctc ccgaactcca cgaaactcaa
    14701 gtataatcac atcgttacaa gcaatctgaa catcctgaac tccaccgaac tctactgaac
    14761 tctttctcaa tcaagctttc attgggggca ccggtggggg ctcattatgt tcagtcagtt
    14821 tcaggagccc ccatatggca cttacagcca gacaggtcga aacagccaga cctaaagaaa
    14881 aagactataa actctctgac gaacgtggtt tatatctgct ggtaaaaacc acgggagccc
    14941 gctactggcg gcttaaatac cggatagcag gaaaagagaa aaaactggcc ctcggcgtct
    15001 atcccgacgt ctcccttgct gaggccagaa tcaaacgcga cgatgcccga aaaatcatct
    15061 ccgaaggtgg tgacccgggc gaaaagaagc gaaaggaaaa actcactcag aaaatctctg
    15121 ccaccaatac gttccatgcc ctcgctacgg aatggcacca gcataaatct ttgtcatggt
    15181 ctgaaagtta cgccagaagc gtactggaag cgctggataa agatattttc ccgtatctgg
    15241 gcaaacgaag cgttacggat atcctcccgc tggaaatgct ggaaattctg cgccgcatag
    15301 aaaaacgtgg ctcgctggaa aaactgcgta aggtacgtca atactgtaat cagatttttc
    15361 gttatgccat cgccaccgga cgagccactg tcaatccggc atctgaactg accagtacgc
    15421 tggcggcgcc aaaagctgca cacttccccc acctgagagc agatgagctc cctgtttttc
    15481 tcaggaagct cgctgaatat catggcagtc ctgttacccg catggcgaca aatctgctgc
    15541 ttctgacagg cctcagaacg attgagctac ggtccgctga atggtcagaa attgattttg
    15601 ataatgccct gtggacaatc cctgaaagcc gcatgaaaat gcgacgtaaa catgtcgtac
    15661 cactgtcacg acaggtcact gacattctgc aacaactcaa aactttctcc ggacaatacc
    15721 ggctggtttt cccgggacgt tgtgatatca acaagccaat gagcgaagcc agcatcaata
    15781 tggtgctcaa acgtatcggt tacgatggca gggcaaccgg tcatggtttt cgtcacacca
    15841 tgagtaccat tctgcacgaa caggggttta attctgcctg gattgaaatg cagttagctc
    15901 atgtggataa aaattccatc aggggtacct ataatcatgc cctgtatatc gatggtcgcc
    15961 gtgaaatgat gcagtggtat gctgactaca ttgattcgct ttctgagcaa acctgatgaa
    16021 tcagtgacat aaaaataatg aataaccatc tggccccgaa agccggatgg ttttatttat
    16081 gttttctttt ttatttgctc acatcttctg aactgcccct gcagacaata atctgaacac
    16141 cagatataga gccactcatg aaataaagca taattatatt gaataacaaa ttacattttt
    16201 tgttacatcc attccatatc tgtcgctggt tgattgccag cgacgcgccg tgatgcatga
    16261 tagaagaatg agataataag agaagaaaag tatgattcgg gttgtgctgg tggatgacca
    16321 tgtggtggtg cgctctggct tcgcacaatt actctctctg gaagacgatc tggaggtcat
    16381 tggtcagtac agtagtgccg cacaggcatg gccggcatta atccgtgatg acgtcaatgt
    16441 tgccgtcatt gatattgcta tgccggacga aaacggcctc agtttgctta agcgcctgcg
    16501 tgcacaaaaa ccgcaatttc gcgccattat tcttagcatt tatgacgcgc caacctttgt
    16561 gcaaagcgcg ctggatgctg gtgccagcgg ctatttgacc aaacgctgtg ggccggaaga
    16621 gttagtacag gcggtgcgct cggtcgggct gggcgggcat tatctctgtg ccgatgcaat
    16681 tcgggcttta cgcggcggcg ggcaacccgc ccaggcgctg gaaattctca ccccacgaga
    16741 acgtgaagtg tttgatttac tggtcaaagg tgacagcgtt aaagagatcg cctttaagct
    16801 cgaactcagt cataaaacgg tacatgtgca tcgcgccaat gtgctgggaa aactgaactg
    16861 ccatagtacc attgagctgg tgcattttgc gctcgaccac catctgctgg cggggcatta
    16921 atgtcgcgca accttcgtca ttgggtgatt tctctgttta ttgtgctggc ctggggaact
    16981 ggctggctga tgctgtggac gctcagcttt tatctgacca ataacggtca acaggcggtg
    17041 ttgtttctcc cgcagggggt ttacctcgcc cttcttattt tgctctctcg ccgctactgg
    17101 cctgcgctgg tgctcccacc tttgctgatg atgttctggc tccatagtga acagctattg
    17161 aacggttatc tcatgctggc aacgccggtt attagcctgt ttcctgccat ccttgcgcaa
    17221 aacttctggc accgttttcc actttactgg cagcgtctga cgttgctgtt agccactgtc
    17281 acggcagctt cattgttgaa taccgccctg ctctcccctt ttatgtcagg cccaataatg
    17341 ctgccggggt tgacctcatt taccggcggc gtattactga cgccgtttgt ctatctcatc
    17401 tttgagtttc tgcgccagca gcatcgctac cagctcctgg ggcttgacac gcataatccc
    17461 cccttgcgta cctctttaat catctggtgt agcctgtttt ttatcattgg tatcggcact
    17521 cagatggtgc tgtcacctga aattgaacgc ctgctgttga tcgtagtttt tctacccaat
    17581 gtggtgatgg cgtggaaatt tggctggcaa ggtggcgtac tctccgggct tttgggcagc
    17641 atgatgatca ccatcgcccg ccagattggc gtgggcttta gtaacctggt ggagctggag
    17701 attttcctcg cgacccaggc attgctggga attggcctgg gcattgccat cagtcgccag
    17761 caacatctgg cgctaaacct gcaccattac cgacaacggc tggaggccga gctggcagcg
    17821 cggcgggcat tggcggaaaa gctgatccac accgaggagg acacgcgcaa aaacctggcg
    17881 cgagagctgc acgacgaaat cggtcagaac atcaccgcca ttcagatcca gtcgcaactg
    17941 gtgaagcggg cgcgcgatcc ggcgcaaatt caatccgcag ccagccaaat caatgaactc
    18001 gcccgccgca ttcatctctc cacccgccag ttgctgcgcc agttgcggcc tcccgcgctt
    18061 gatgaactga cattccgcga agcgttgctg catctgatta atgaatttgc ctttagtgaa
    18121 cgcggcattc attgccagtt tgcctatcaa ctgaactcca caccagaaaa cgaaacggtg
    18181 cgctttacct tgtatcggtt attgcaggag ttgctcaata acgtctgtaa acacgccgaa
    18241 gccagcgaag tgacgattat cctgcgccag cagggagagg tattgcatct ggaggtcagc
    18301 gacaacggtg tggggatcgc cagcgggaaa atggccggat ttggcattca ggggatgcgt
    18361 gagcgcgtca gcgccctggg cggcgactta acgctcgaaa aacagcacgg cacgcgggta
    18421 attgttaact tgcccacatt tttgcaacaa atcgccggat aaccaggaaa aagtcttagg
    18481 tatcccggat tttctctcat cccctttcac tttcattttc atatagtcag caacatggag
    18541 acaaccatgc acgcgcgttc agcgagagaa attaatcaat gctaccggac attacgtccg
    18601 cgattgctga tgtgcatgat tattggctac gccgcatttt atctgacgcg aaaaagcgtg
    18661 aattacgtcc ttccggcgct gcaaaccgac ctcggactgg ataaaggcga cattggcctg
    18721 ctgggttcgc tgttttatct cagctacgga ttgtcgaaat ttaccgccgg actgtggcat
    18781 gacagccacg gacaacgcgg ttttatgggc gtcggcttgt tcgccaccgg gttattgaac
    18841 gtggtgtttg cctttggcga atcacttacg cttctgcttg tggtctggac actgaacggt
    18901 ttttttcagg gctggggatg gccaccgtgt gcccgcttgc tgacccactg gtactcacgc
    18961 aatgagcgcg gtttctggtg gggctgctgg aatatgtcga tcaacattgg cggagcgatt
    19021 attccgctga ttagcgcctt tgccgcccac tggtggggct ggcaggccgc gatgctgaca
    19081 ccggggataa tcagcatggc attagggatc tggttaacgc tacaactcaa aggcacgccc
    19141 caggaagagg gattaccgac agttggtcac tggcgtcacg atccgctgga gttacgtcag
    19201 gaacaacaaa gtccgccgat ggggctgtgg caaatgttgc gcaccaccat gctgcaaaat
    19261 ccgctgatct ggctactggg cgtctcttat gtactggttt atgtgatccg gattgcgctt
    19321 aacgactggg gcaatatctg gctgacggaa agccacggcg tcaacctgct caacgccaac
    19381 gccacggtaa tgctgtttga agtgggcggc ttactcggcg cgttatttgc cggatgggga
    19441 tcggatttgc tgtttagcgg gcaacgagcg ccaatgattt tgctgttcac gctggggttg
    19501 atggtgtcag tcgccgcact gtggctggca ccggttcatc actacgctct gctggcagtg
    19561 tgctttttta ccgtgggctt tttcgtcttt ggtccacaaa tgctgattgg acttgcggcg
    19621 gtggagtgtg ggcacaaagc tgcggcgggt tcaatcaccg gatttctcgg cctgttcgct
    19681 taccttggcg cggcgcttgc gggctggcca ctgtcgctgg tcatcgaacg ctacggctgg
    19741 tcgggaatgt tcagtttact ctcggtcgcc gcagtactta tgggtttact gctgatgccg
    19801 ttactgatgg cgggcatcac caccactcac gctcgaagga taaaacaatg aaaatgacct
    19861 ttacctctac tctgattgct tctgccgttg cgctggcgac tttaacgggt gccgcgcagg
    19921 cgaaaggccg gctggtggtc tattgcagcg ccaccaatga aatgtgtgag gccgaaacca
    19981 aagcgtttgg cgagaaatat gatgtaaaaa cctcgtttat ccgcaacggt tccggaagca
    20041 ccctggcaaa agtcgatgct gaaaagaaaa acccccaggc tgacgtctgg tacggcggca
    20101 ctttagaccc gcaatcgcag gctggcgaga tgggcctgct acaggcgtat aaatccccta
    20161 accttgagca gatcatggag aaattccgcg atccggcaaa agtgaaaggg aatctgtcct
    20221 ctgccgtata tgtggggatc ctcggttttg gcgtaaacac cgatcgtctg aaagagaaaa
    20281 acctgccggt gccgaagtgc tggaaagatc tcaccaaacc ggaatataaa ggcgaaattc
    20341 aaattgccga tccgcaaagt tccggtactg cctacaccgc cctggctact tttgcgcagc
    20401 tgtgggggga agatcaggcc tttgattatc taaaacaact gaacgccaac gtctcccagt
    20461 acaccaaatc aggcatagcc ccggcacgta acgccgcccg tggcgaaacg gcgattggta
    20521 ttggcttcct gcatgactac tcgctggaaa aagaacaggg cgcgccgctg gagttgattt
    20581 ccccgtgtga aggtacgggc tacgaaattg gcggcgtcag cattctgaaa ggcgcgcgca
    20641 acctcgacaa cgccaaactg tttgtggact gggtattatc aaaagaagct caggaactgg
    20701 cgtggaagaa agggaagtct tatcagatcc tgaccaatac caccgccgaa acgtcgccaa
    20761 attcgctgaa gctcgacgac ctgaaattaa tcaactacga catggataaa tatggttcca
    20821 cggatgttcg taaggcattg attaataaat gggtcagcga agtgaagatg ggtaaataaa
    20881 cccgcattag gcgcatttgc cgggtgcagc acaaacgcct gccccggcca accataccgg
    20941 aactgctatg tcacagacac ttgcactcca tcccgtgaaa aagcgggatg cgattttcct
    21001 ttgggttttg ctgggctggc tggcatttat cctgttgccg agctggagcc ttgattatgg
    21061 cctgctggag tccacgagtg atgagatcct cgaggcttat ggctggtcgc attttaatat
    21121 cagctggctg tggtatttat taccgagtct gttgctggtt cgtccgtttc gtgaggccac
    21181 acgcgaacag cgtagtcgtc attattttga tgccagctgg gcattgctct gtatggcgtt
    21241 tatcgtcatc agcgccaccg tggctggacg cggtttaggc tacgccgcca tcgtgctgtt
    21301 tgttgaatta ggcgcgatta tgacgctggc cttaacccgc ctcgaatggc tgggcggcga
    21361 ccgcttcgtg atcggctcac tgattgcgat tgtggcgctg attggcattt ttatcgtctg
    21421 gccgagcatc gccattttta tcccgatgtt caccaacgac gcgggagagt tcgccccgct
    21481 ggcgtttatg aacgtgctct cgcaggcaca tattgtgcag gtgatcgtca actctatcgt
    21541 cttgtcgata gcggtgggta ttggctgtac cttcttcggc ctggtgctgg cgatttatac
    21601 cacgcgcatc gccaaacgca gcgccattat tggccgcatt ttttccattc tgcccatcgt
    21661 tacgccacct tttgtggtcg gcttaggcgt gacgctcatg atggggcgct ccggctacat
    21721 aacggagttt atggtggagt ggtttgggct gaccaacacc aactggctgt atggttttac
    21781 cgggatctgg ctggcgcagg ttctggcatt cacgccaatg gcttttatga tcctcgacgg
    21841 ggccattaaa accattcatc cctcgctgga agaagcctcc tataccctgc gcgccagccg
    21901 ctggcaaacc tttaacggcg tgtttgtgcc attgctaaag cccgcgctcg ccaatgcctt
    21961 tttaatcgtc gtggtgcagt cactggctga tttcagtaac ccgctggtgc ttggcggcaa
    22021 cttcgacgta ctggcgacgc aaatttactt ctacattacc ggctcgcagc tggattatca
    22081 ggccgccagc acgcttggtg ctttcctgct gctgttctcg ctgctggtgt tctgcgtcca
    22141 gtatatgtgg attggtaaac gctcttacgt cacggtttcc ggcaaatcct accgtggcga
    22201 cgtccagccg ctaccggtca ccctggtgtg gagcgtggtc gccatactta cggtatggat
    22261 tgcctttaac gccctgctct acggcagcat tttctacggc agcttcacgg tgaactgggg
    22321 cgttgactac accctgacgc tggataactt tatcaagtta tttggtcagg ggatgagcga
    22381 cggcgcgtgg ccttcactgc tggataccct gctctacgcc gggattgcag cccccattac
    22441 cgctatcttt ggcctgctca tcgcctgggt ggtggtgcgt caacaattca aaggcaaaaa
    22501 gaccatcgag ttcaccacca tgctgtgctt tgccgtaccg ggtacagtgg cgggtgtctc
    22561 gtatattctt gcctttaata gcgccccact ctatctcgcc ggaacggcag caattgttat
    22621 tatctcgatg gtgatgcgta acgttccggt gggtattcgc gccgggattg caggtctggg
    22681 gcagatcgac aaatcgctgg atgaagcctc acttagcctg cgtgcgggtt ccctgcgaac
    22741 cattacgcac attttactgc cgctgttacg cccggccatt ctctcggccc tgatttacag
    22801 ctttgtgcgg gccatcacta cagtcagcgc catcgtcttt ctcgtcacac cggacacacg
    22861 cgtggcaacc gcctacatcc ttaaccgcgt agaagacggc gaatatggcg tagccatcgc
    22921 ctacggttcg attctgatcg tggtgatgct ggcgattatt ttcctctttg actggctgat
    22981 cggtgaagca cgtatttccc gttctaaagc caaaaaccag gcgtaatgga gcgcactatg
    23041 agtcagaaaa attttgtgga actgcgcaac gtcactaaac gattcggcag taatagggtt
    23101 atcgacaata tcaatctcac catcccacaa gggcaaatgg tgacgctgct cggtccttcc
    23161 ggatgcggca aaaccaccat tctgcgtctg gttgccgggc tggaaaaacc gagcgaaggg
    23221 caaattttta tagatggcga agatgtcacg catcgttcca ttcagcaacg cgatatctgc
    23281 atggtgtttc agtcatacgc tctgttcccg catatgtcgc tgggcgaaaa cgttggctat
    23341 gggctgaaaa tgcttggcgt gtcgcgtagt gaagtgaagc agcgggtgaa agatgcgctg
    23401 acaatggtgg atctggaagg attcgaagac cgctatgtcg accagatttc cggtggtcag
    23461 caacagcgtg tggctctggc ccgtgcgttg atcctcaagc caaaggtgct gctgtttgat
    23521 gagccgttaa gtaacctcga tgccaacctg cgccgcagca tgcgcgataa gatccgcgag
    23581 ctgcaaaagc agtttaatat cacgtcactc tacgtgactc acgatcaaag tgaagctttc
    23641 gcggtgtccg acactgtgct ggtgatgaat aaaggtcaca tcatgcagat tggctcaccg
    23701 caggatctct atcgtcagcc agcctcccga tttatggcaa gttttatggg cgacgccaac
    23761 ctgttcccgg cgaactttag cgaagagtat gtcgatatct acggttatcg cctgccgcgc
    23821 gcagcgcatt tcccggcgca aggtagcggc accgccggcg ttcgcccgga agccatcacc
    23881 ttaaacaatc acggcgaaga gagtcagcgt tgtgttattc gccatgtcgc ttacatgggg
    23941 ccgcagtacg aagtgaccgt agaatggcat ggtcaggaga ttttattgca agtcaacgcc
    24001 acccgtttac agcccgatat tggtgagcac tattacctcg aaatccaccc ttacgggatg
    24061 tttgttttag cggatgcggc ataaaaatag cgcataagcg gtcccctcca gctcagctgt
    24121 ctcttataca caaatcacca cctggggatt tgtgatgtct gtcagtctgc tttagggggg
    24181 actctttcca tccggtagtt tccttcctcc ccggcagcga ttttttgaac tttttcttca
    24241 cccgttgatc ttctcttata tttcattttc ttcgggtctg ttatggtact ttctgattgt
    24301 tattcatggg ctaacgagca gtctggacat gcccgtctcg gtgatccgcg tcgtacacga
    24361 cgccttgtgt cactggcctc ttctctcgct cagcatgcag gtctttctat tgtgaaataa
    24421 tcccactcca ctgctcaggt tgaaggtgcg taccgcctga tgcgtaaccc gtcagtgtcc
    24481 ccggaagcca tttccggagc cggatttacc gccactgcaa gggcatgtga ggcacatccg
    24541 ctgctccccg cactggaaga cacctccacc attaacttca gccacagtac ggcatctgat
    24601 gacctgggga ataccaccac taacccgaag acgcgcgggc tgctggctca ctctgtcctg
    24661 atatacgccc ccgacagcgc cctgccagtc gctcttatcg aacagcagag ctggagtcgg
    24721 gccacggaca cctacggtgt taagcaccag cgaaaagaac gtccctgtga agaaaaagaa
    24781 agttaccgct ggcagcaggc ttcggagcgc atggccgaac ggctggggga gatacagaaa
    24841 cgggtaatca ctgtctgtga ccgggaagcg gatatctggc attacctgca ttataaagtc
    24901 agccacggac agcgcttcgt ggtgcgtgct gcacagaata gccggctgga agaagcaccc
    24961 ggcaagctct tcgaactgcc ggaagtcctg gcaaccgcgg gaagccacac gctgaatgtg
    25021 atgcaaaaag gcgggcgggc agcccgccag gcccggatgt tcatcagtta cagcgaagtc
    25081 agcataaaaa atcccgacaa cagcggccag gcgctctcgc tcacgtatgt ctgttgccgg
    25141 gagcaggcag aggacagtgc ctgctggcat ctgctgacgt cagaaaaagt ggcgagtgcc
    25201 gccgatgcgc gacgtatcgt cagccattac gagcgacgct ggctgataga ggaataccac
    25261 aaggcgtgga aaagtggtgg tacatgtgtg gaatcgctgc gaattcagac ccgggataac
    25321 cttgagcgca tggtggttat caaggcgttt atcgcggtac gggtgccggg cctgcggcag
    25381 ggaggtgtca gtgaagaaac acaaaatgac agttgtgaga aaatactgac gccgacagag
    25441 tggaaactgc tgtgggttaa gctggaagga gagccgcttc cggcgcaggc accgatcctg
    25501 aaatgagcca gtctgaaact ggctaaactg gggcgatgac atgacagcaa acgcacaggc
    25561 cgccccggtt gggtagttat gtgggatgac tggttcagac ttcaggatat ggttgagggt
    25621 tacctggtga
//
LOCUS       DAZKGF010000064        25611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZKGF010000064 DAZKGF010000000
VERSION     DAZKGF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.064"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..75
                     /locus_tag="ACHOQV_002422"
     CDS             <1..75
                     /locus_tag="ACHOQV_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630787.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
     gene            336..677
                     /gene="raiA"
                     /locus_tag="ACHOQV_002423"
     CDS             336..677
                     /gene="raiA"
                     /locus_tag="ACHOQV_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP9630788.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    781..906
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            782..829
                     /gene="pheL"
                     /locus_tag="ACHOQV_002424"
     CDS             782..829
                     /gene="pheL"
                     /locus_tag="ACHOQV_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFP9630789.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            928..2088
                     /gene="pheA"
                     /locus_tag="ACHOQV_002425"
     CDS             928..2088
                     /gene="pheA"
                     /locus_tag="ACHOQV_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFP9630790.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPQWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGDITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2131..3252)
                     /gene="tyrA"
                     /locus_tag="ACHOQV_002426"
     CDS             complement(2131..3252)
                     /gene="tyrA"
                     /locus_tag="ACHOQV_002426"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFP9630791.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAAEIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRH"
     gene            complement(3263..4333)
                     /gene="aroF"
                     /locus_tag="ACHOQV_002427"
     CDS             complement(3263..4333)
                     /gene="aroF"
                     /locus_tag="ACHOQV_002427"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFP9630792.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4543..4908
                     /gene="yfiL"
                     /locus_tag="ACHOQV_002428"
     CDS             4543..4908
                     /gene="yfiL"
                     /locus_tag="ACHOQV_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFP9630793.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     NAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            5058..5576
                     /gene="yfiR"
                     /locus_tag="ACHOQV_002429"
     CDS             5058..5576
                     /gene="yfiR"
                     /locus_tag="ACHOQV_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFP9630794.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5566..6792
                     /gene="dgcN"
                     /locus_tag="ACHOQV_002430"
     CDS             5566..6792
                     /gene="dgcN"
                     /locus_tag="ACHOQV_002430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFP9630795.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNRPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            6808..7290
                     /gene="yfiB"
                     /locus_tag="ACHOQV_002431"
     CDS             6808..7290
                     /gene="yfiB"
                     /locus_tag="ACHOQV_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9630796.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     ALSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7367..7714)
                     /gene="rplS"
                     /locus_tag="ACHOQV_002432"
     CDS             complement(7367..7714)
                     /gene="rplS"
                     /locus_tag="ACHOQV_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP9630797.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7756..8523)
                     /gene="trmD"
                     /locus_tag="ACHOQV_002433"
     CDS             complement(7756..8523)
                     /gene="trmD"
                     /locus_tag="ACHOQV_002433"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP9630798.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8554..9102)
                     /gene="rimM"
                     /locus_tag="ACHOQV_002434"
     CDS             complement(8554..9102)
                     /gene="rimM"
                     /locus_tag="ACHOQV_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP9630799.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9121..9369)
                     /gene="rpsP"
                     /locus_tag="ACHOQV_002435"
     CDS             complement(9121..9369)
                     /gene="rpsP"
                     /locus_tag="ACHOQV_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP9630800.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9506..10867)
                     /gene="ffh"
                     /locus_tag="ACHOQV_002436"
     CDS             complement(9506..10867)
                     /gene="ffh"
                     /locus_tag="ACHOQV_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP9630801.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            11034..11825
                     /gene="ypjD"
                     /locus_tag="ACHOQV_002437"
     CDS             11034..11825
                     /gene="ypjD"
                     /locus_tag="ACHOQV_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFP9630802.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11846..13132
                     /gene="yfjD"
                     /locus_tag="ACHOQV_002438"
     CDS             11846..13132
                     /gene="yfjD"
                     /locus_tag="ACHOQV_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFP9630803.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13186..13779)
                     /gene="grpE"
                     /locus_tag="ACHOQV_002439"
     CDS             complement(13186..13779)
                     /gene="grpE"
                     /locus_tag="ACHOQV_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP9630804.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13902..14780
                     /gene="nadK"
                     /locus_tag="ACHOQV_002440"
     CDS             13902..14780
                     /gene="nadK"
                     /locus_tag="ACHOQV_002440"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFP9630805.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14866..16527
                     /gene="recN"
                     /locus_tag="ACHOQV_002441"
     CDS             14866..16527
                     /gene="recN"
                     /locus_tag="ACHOQV_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP9630806.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKHLA
                     NSGQLLTTSQNALAVMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELALLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16676..17017
                     /gene="bamE"
                     /locus_tag="ACHOQV_002442"
     CDS             16676..17017
                     /gene="bamE"
                     /locus_tag="ACHOQV_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP9630807.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(17079..17369)
                     /gene="yfjF"
                     /locus_tag="ACHOQV_002443"
     CDS             complement(17079..17369)
                     /gene="yfjF"
                     /locus_tag="ACHOQV_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFP9630808.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17359..17796)
                     /gene="ratA"
                     /locus_tag="ACHOQV_002444"
     CDS             complement(17359..17796)
                     /gene="ratA"
                     /locus_tag="ACHOQV_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFP9630809.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17967..18449
                     /gene="smpB"
                     /locus_tag="ACHOQV_002445"
     CDS             17967..18449
                     /gene="smpB"
                     /locus_tag="ACHOQV_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP9630810.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18664..19026
                     /gene="ssrA"
                     /locus_tag="ACHOQV_002446"
     tmRNA           18664..19026
                     /gene="ssrA"
                     /locus_tag="ACHOQV_002446"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(19187..23761)
                     /gene="ypjA"
                     /locus_tag="ACHOQV_002447"
     CDS             complement(19187..23761)
                     /gene="ypjA"
                     /locus_tag="ACHOQV_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFP9630811.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQKVDERGTTNNTHIINHGQQNVYGGISNGSLIESGGYQDVGGHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLDSKSTAKNTQIYSGGTQIVDYTSTSDVIEIYSGGVLDVRGGM
                     ATNVTQHDGAALKVTTYDLTVSGTNSEGAFSIHNNVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTNISSGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVMAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATDANIESGEQIVDGGSTDKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLTNGGVLTVESDTTSAKTQVNA
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSIAGYLATNVLLENGGNLLV
                     LAGTEARDSTVGNGGAMQNLGQDFATKVNSGGQYTLGRSKDEFQVLARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTT
                     SELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPKPDPKPDPKPDP
                     KPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNN
                     VWGTTYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHTHIFFD
                     RGGHGSVGSYSLGGYASWEHESGFYLDGIVKLNRFESNVAGKMSSGGAANGSYRSNGL
                     GGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLS
                     YNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSS
                     SLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(23826..23945)
                     /locus_tag="ACHOQV_002448"
     CDS             complement(23826..23945)
                     /locus_tag="ACHOQV_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP9630812.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(24100..24366)
                     /locus_tag="ACHOQV_002449"
     CDS             complement(24100..24366)
                     /locus_tag="ACHOQV_002449"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9630813.1"
                     /translation="MLRVTTSAHNRGSRPGQNKRLRTLSVGDALLVCKLNRLGRSMWH
                     LVVLLEELCERGINFRALAQSIFAQQWGDGSCKSKTICDLRVIV"
     gene            complement(24697..25611)
                     /locus_tag="ACHOQV_002450"
                     /pseudo
     CDS             complement(24697..25611)
                     /locus_tag="ACHOQV_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961341.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6506 a         6693 c         5828 g         6584 t
ORIGIN      
        1 gccttcccga gagtggtaaa ggagaggccc tggaaatacc ccacagcccc gaaaaagagc
       61 cagtcagttg cttaactgat cggcattaca gcccttgagc tgccgttttt ttattctgtt
      121 agttgtgaaa ctgaagcgat ttagtcacta tcgatctcat caaatatggc tcgctttgag
      181 atattcctca agtaaaaaaa cacctcttcc tgcgatttct cacaaaaaag attcgttgac
      241 aaaaagtgac aaaattatga gatttccatc acacattttg acatcaggaa cggtatgctg
      301 aattcaccaa gacgggaaga caagaggtaa aatttatgac aatgaacatt accagcaaac
      361 aaatggaaat tactccggcc atccgccaac atgtcgcaga ccgtctcgcc aaactggaaa
      421 aatggcaaac acatctgatt aatccacata tcattctgtc caaagagcca caagggtttg
      481 ttgctgacgc cacaatcaat acacctaacg gcgttctggt tgccagtggt aaacatgaag
      541 atatgtacac cgcaattaac gaattgatca acaagctgga acggcagctc aataaactgc
      601 agcacaaagg cgaagcacgt cgtgccgcaa catcggtgaa agacgccaac ttcgtcgaag
      661 aagttgaaga agagtagtcc tttatattga gtgtatcgcc aacgcgccct tcgggcgcgt
      721 tttttgttga cagcgtgaaa acagtacggg tactgtacta aagtcactta aggaaacaaa
      781 catgaaacac acaccgtttt tcttcgcatt cttttttacc ttcccctgaa tgggaggcgt
      841 ttcgtcgtgt gaaacagaat gcgaagacga acaataaggc ctcccaaatc ggggggcctt
      901 ttttattgat aacaaaaagg caacactatg acatcggaaa acccgttact ggcgctgcga
      961 gagaaaatca gcgcgctgga tgaaaaatta ttagcattac tggcagagcg acgcgaactg
     1021 gccgtcgagg tgggaaaagc caaactgctc tcgcatcgcc cggtacgtga tattgatcgt
     1081 gaacgcgatt tgctggaaag attaattacg ctcggtaaag cgcaccatct ggacgcccat
     1141 tacattactc gcctgttcca gctcatcatt gaagattccg tattaactca gcaggctttg
     1201 ctccagcaac atctcaataa aattaatccg cactcagcac gcatcgcttt tctcggcccc
     1261 aaaggttctt attcccatct tgcggcgcgc cagtatgctg cccgtcactt tgagcaattc
     1321 attgaaagtg gctgcgccaa atttgccgat atttttaatc aggtggaaac cggccaggcc
     1381 gactatgccg tcgtaccgat tgaaaatacc agctccggtg ccataaatga cgtttacgat
     1441 ctgctgcaac ataccagttt gtcgattgtt ggcgagatga cgttaactat cgaccattgt
     1501 ttgttggtct ccggcactac tgatttatcc accatcaata cggtctacag ccatccgcag
     1561 ccattccagc aatgcagcaa attccttaat cgttatccgc aatggaagat tgaatatacc
     1621 gaaagtacgt ctgcggcaat ggaaaaggtt gcacaggcaa aatcaccgca tgttgctgcg
     1681 ttgggaagcg aagctggcgg cactttgtac ggtttgcagg tactggagcg tattgaagcg
     1741 aatcagcgac aaaacttcac ccgatttgtg gtgttggcac gtaaagccat taacgtgtct
     1801 gaccaggttc cagcgaaaac gacgttgtta atggcgaccg ggcaacaagc tggtgcgctg
     1861 gttgaagcgt tgctggtact gcgcaaccac aatctgatta tgacccgtct ggagtcacgc
     1921 ccgattcacg gtaatccatg ggaagagatg ttttatctgg atattcaggc caatcttgaa
     1981 tcagcggaaa tgcaaaaagc attgaaagag ttaggggaca tcacccgttc gatgaaggta
     2041 ttgggctgtt acccaagtga gaacgtagtg cctgttgatc caacctgatg aaaaggtgcc
     2101 ggatgatgtg aatcacccgg cactggatta ttagtgacgg ttgtcattcg cctgacgcaa
     2161 taacacgcgg ctttcactct gaaaacgctg tgcgtaatcg ccgaaccagt gctccacctt
     2221 gcggaaactg tcaataaacg cctgcttatc gccctgctcc agcaactcaa tcgcctcgcc
     2281 gaaacgctta tagtaacgtt tgattaacgc cagattacgc tctgacgaca taataatgtc
     2341 ggcatacagt tgcggatcct gggcaaacag tcgcccaacc atcgccagct caaggcggta
     2401 aatcggcgaa gaaagcgcca gaagttgctc aagctgaaca ttttcttctg ccaggtgcag
     2461 cccataagca aaagtagcaa agtggcgcag tgcctgaata aacgccatat tctgatcgtg
     2521 ctcgacagcg ctaatacgat gcagccgagc gccccagacc tgaatttgct ccagaaacca
     2581 ttggtatgct tccggcttac gtccatcaca ccagaccaca acttgttttg ccaggctacc
     2641 gctgtccggg ccgaacatcg ggtgtaaccc cagcaccgga ccatcatgcg ccgccagcat
     2701 ggcctgtaat ggtccatttt tcaccgatgc cagatcgacc agaatacaat ctttcggtaa
     2761 aggcggtaat ttgccaataa cttgttcagt aacgtggatg ggcacactaa caatcaccat
     2821 tccggcatcg gcaactatct cagccgctcg atcccagtca tgttgctcca gaatccgcac
     2881 ctgataaccc gatagtgtca gcatcttctc aaacaggcgt cccatctgac cgccaccgcc
     2941 gacgataacc accgggcgca gtgacggaca aagcgtttta aatcctttgt cgttttcact
     3001 ggagtaagat tcacgcatca cccgacgcaa aacatcctca atcagatctg gcggtacacc
     3061 cagagcttcc gcctctgcac gacgcgaggc caacatagat gcctcgcgct ccggaacata
     3121 aataggcagt ccaaagcggc ttttcacctc gcccacttca gcaaccagtt ccagacgctt
     3181 cgctaataaa ttcagcagcg ctttatcgac ttcatcaatt tgatcgcgta atgcggtcaa
     3241 ttcagcaacc ataataaacc tcttaagcca cgcgagccgt cagctgcccg ttcagatcct
     3301 gatgaatttc acgcagcaag gcatcggtca tttcccagct aatgcaggca tcggttacgg
     3361 atacaccgta tttcatttca ctgcgcggtt gctcggaaga ctgattgccc tcgtggatat
     3421 tactttcgat catcagacca ataattgagc gattgccatc tttgatttga gcaaccacgg
     3481 attctgccac cgcaggctga cggcgataat ctttattgga attaccgtgg ctgcaatcta
     3541 ccatcagaga cgggcgcagt cccgcctgtt ccatctcttt ttcacattgc gcaacatccg
     3601 cagggctata gttcggcgct ttaccaccgc gcaggatcac atgcccgtcc ggattcccct
     3661 gagtttgtag caacgccacc tgccctgcct ggttaatgcc aacaaaacgg tgcggctggg
     3721 cggcggcacg catagcgtta atagccgttg caaggctgcc gtcggtgccg tttttaaaac
     3781 caaccggcat ggaaagcccg gaggccattt cacggtgtgt ttgcgattcc gttgtacgag
     3841 caccaattgc tgaccagcta aacagatcgc ccaggtattg cgggctattc ggatctaacg
     3901 cttccgtcgc cagtggcagt cccatattca ccagctcaag cagcaattta cgcgcgatct
     3961 gcagcccggc ttctacatca aaagagccat ccatatgggg atcgttaatc aaccctttcc
     4021 aaccgacagt ggtacggggt ttttcaaaat agacgcgcat taccagatag aggctatcgc
     4081 tgacctctgc ggcaagggct ttaaatcgac gagcatattc cagagcagtt tccggatcat
     4141 gaatggaaca aggaccacat actaccaaca gacgaggatc gcgcccggcg ataatatctg
     4201 aaatggtttt acgcgagtca gcaatctggg cttcctgttg caggcttaat ggaaaagcgg
     4261 ccttcagttg ttccggagtc attaaaactt gttcgtcggt aatatgtacg ttattcagcg
     4321 cgtctttttg catgatggcg atcctgttta tgctcgtttg cgatagttga tcctcagcga
     4381 ggatgacgta acgataacac ataaagtaaa gttttcaatc catatttcgt acatttttct
     4441 ttacacaagc aatttattcg cgcttttaac ccttgtttat gtataaataa atttacacca
     4501 cctttgaaaa caatccgcta tgctttgaaa aaggagaaag aaatgatgaa aaagtttatc
     4561 gcccccttgt tggctttact ggttagcgga tgtcagattg atccttatac tcacgcgcca
     4621 accttgacca gcaccgactg gtatgatgtt ggtatggaag atgcgatatc gggcaacgcc
     4681 ataaaagatg acgatgcatt tagcgattca caggcggatc gcggtctata ccttaaagga
     4741 tatgccgaag gacaaaagaa aacttgccag accgatttta cttatgcccg aggactttcc
     4801 ggtaaaagct ttcctgccag ctgcaacaat gttgaaaatg ccagccaact gcatgaagta
     4861 tggcaaaaag gggctgatga aaatgccagc acaatacgat taaattaatt aaacccccac
     4921 aaataaatta aacatgagat tctactcatt gtgttttatt tctcacattg atgacgaccg
     4981 catgaaagaa tgatatttta atgagcattc tgaaaaaata tcgatgcatt tcgagcgaag
     5041 atggtgagga tccctgaatg cgtttttctc accgactgtt cttactgctg attctcctgc
     5101 tgacgggagc accaatactc gctcaggaac cttctgacgt tgcgaaaaac gtgcgtatga
     5161 tggtttccgg tattgtcagt tacacccgct ggcctgcgct atcagggcca ccaaagttat
     5221 gtatcttttc atcttcacat tttagtaacg ccctacaaga gaatggcacg acatctttgc
     5281 cttatctacc tgtcatcatt cacactcaac aagaggcgat gatttcaggc tgcaatggtt
     5341 tttattttgg caacgagtca ccgacattcc agatggaatt aaccgaacaa tatccgtcaa
     5401 aggcgttatt attaattgcc gaacaaaata ccgagtgcat tattggtagc gccttttgtc
     5461 taatcatcca caataatgac gtcagatttg ccgtaaacct ggatgcctta tcgcgtagcg
     5521 gtgtaaaggt caacccggat gtcttaatgc tcgcacggaa gaaaaatgat ggataacgat
     5581 aattctctta ataagcgccc cacgtttaaa agagcattac gcaacatcag tatgaccagc
     5641 atatttatca ctatgatgct gatctggttg ctgctttccg tgacctcggt gctgaccctg
     5701 aaacagtacg cgcaaaaaaa cctggcactg acagcagcaa caatgactta cagtctggaa
     5761 gcagctgtcg tttttgccga tggccctgca gcaactgaaa cactggcagc gctgggccag
     5821 caaggccaat tttcaactgc agaagtgcgt gataagcagc aaaatattct ggcatcctgg
     5881 cattacaccc ataaggaacc aggcgatact ttcagcaatt tcataagcca ctggctcttc
     5941 cctgccccca tcattcagcc gattcgtcac aatggtgaaa ccattggcga agtacgctta
     6001 actgctcgcg acagttcaat cagccatttt atctggtttt cgctcgctgt cctgaccggt
     6061 tgtattctgc tggcatcagg catcgcaatt accctcaccc gccatttgca caatggcctg
     6121 gtggaagcgc tgaaaaatat caccgatgtc gtacatgatg tgcgttctaa ccgcaatttt
     6181 tcccgacgag tttcggaaga acgtatcgct gagtttcacc gcttcgctct cgacttcaac
     6241 agtctgctgg atgaaatgga agagtggcag cttcgtttac aggccaaaaa tgcgcagctt
     6301 ttacgtaccg cgctacatga cccattaacc gggctggcta accgcgcagc gtttcgtagc
     6361 ggcatcaaca cgttgatgaa caattccgat gcccgaaaaa cgtcggcgtt actatttctt
     6421 gatggcgata atttcaaata tatcaatgat acctggggtc atgcgacggg cgatagagtc
     6481 ttgattgaaa tcgcaaaacg gttagctgaa tttggcggac tgcgacataa agcataccgc
     6541 ctgggcggcg atgaattcgc gatggtgctc tatgatgtac agtcggaatc tgaagtgcag
     6601 cagatatgct cagcactgac acaaatcttt aatcgcccgt ttgatcttca taatggtcat
     6661 cagaccacca tgacattaag cattggttac gcgatgacca ttgagcatgc ctctgcggaa
     6721 aaattacaag agctggccga tcacaatatg tatcaggcca aacaccagcg tgccgaaaag
     6781 ctggtgagat aacaaggata tatcgatatg ataaagcacc tggtagcacc cctgattttc
     6841 acctcactaa tactgactgg ctgccagtcc cctcagggaa agtttactcc tgagcaagtc
     6901 gccgctatgc aatcttatgg atttactgaa tccgccggcg actggtcgct gggcttatca
     6961 gatgccattc tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacagatc
     7021 caaaccatgg cagccaaatt ggcctcgaca gggctaacgc atgcccgtat ggatggacac
     7081 accgacaact atggtgaaga cagttacaac gaagccttat cattgaaacg ggcgaatgtc
     7141 gtggccgatg catgggctat gggtggacaa attccgcgca gcaatctcac cacacagggt
     7201 ttaggaaaaa aatatcccat agccagtaac aagaccgccc agggccgcgc cgagaaccgc
     7261 cgcgtcgcag tggtgattac taccccttaa aacacgagca aacagataaa aaaagggcca
     7321 gccaattggc cagcccttct taacaggatg tcgcttaagc gaaatcttag ttaagacgct
     7381 ctttgatacg agcagcctta ccagtacgct cacgcaggta gtacagttta gctttacgaa
     7441 cagcaccacg acgtttgaca gaaatgctgt caactaccgg agagtgagtc tggaagacac
     7501 gctcaacgcc ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc agaccgcggt
     7561 tacgaatagc gataaccacg ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa
     7621 cccatacttt cacttccacg gtatcacccg gacggaagga aggtacgtcc tgcttcatct
     7681 gctcttgttc aagttgctta ataatgttgc tcataattta atctcttatc ctgggtaaac
     7741 tgatatctcg ggggcttacg ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt
     7801 tttgaactcc gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc
     7861 aggtcttcta agccaggtac ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc
     7921 atggttgccc gacagtaaca ctggcggaac ttccatccct tctaacacct caggccgcgt
     7981 atagtgcggg caatccagca atccttcagc aaaggaatct tccgttgccg aggcttcatg
     8041 tcccagtact cccggaataa accgggaaac ggagtcaatc agcgtcattg ctggtaactc
     8101 accaccactg agaacgtaat cgccgattga ccattcttcg tcaatttcgg tttggatcac
     8161 gcgctcatct ataccttcgt agcgaccgca caccagaatc aatttttgat tcgttgccag
     8221 ttcgctgacg cccgcttgat caagcttgcg tccctgtggt gacagataaa tcacctttgc
     8281 gccttcaccc gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat
     8341 taacatcccc ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc ggtcatgcgt
     8401 gaagtcgcga ggactccagc tctggatgct cagcaggcca tttttaactg cccggccagt
     8461 taccccgtaa tcggtaattg cgcggaacat ttcaggaaac aggctaatta tgccaatcca
     8521 catagcgccg tcttttaccg tttatccggt ggtttaaaaa ccaggatccc aatctacttc
     8581 gattgaacga gtagtgagat cgactttctt gataacctgc ccatcgagga acggtacgag
     8641 acgttccttg ataccaaacg catctttcag gtttgcctta atgacgagaa cgtcattaga
     8701 tccggtttcc atcatatcga cgactttacc gagatcgtag ccttcagtgg ttactacctg
     8761 gcagcccatc aagtctttcc agtagtagtc accctcttca agctgaggca actgcgatga
     8821 atccacgaca atttcacaat tcgtcagcag gttcgccgca tcacgatcgt caacgccttt
     8881 cagcttgatg atcatgtcct gattgtggtg cttccagctt tccagctgga cttgctgcca
     8941 ctgacccgcc ttctggataa accagggctg atagtcaaaa atgctttcgg cgtcttcggt
     9001 ggaagaaaac actctgagcc acccacgaat accgtaagac gaacccattt ttcccaaaac
     9061 gatgggatca acaggtgctt gcgcggtgag ttgtttgctc atcatgacca ccgtgacaga
     9121 ttaagctgct ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa tagttgcgcc
     9181 ctggccaacc cagtgagcga tgcgatccag atccaggcga gtgccttctt ctttttcgct
     9241 agcgattggg ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg cattacggct
     9301 gtcagcgaca acaacctggt agaacggacg ctttttagcg ccgtgacgtg ctaaacgaat
     9361 agttaccata acatcctctt gtgtgaataa aacaaccgga ccccatcgag gaacggagtc
     9421 cggtgtcata ttaaaagccc gaaaatttta ctgatttctg gagaaaatgc aatcagcagt
     9481 taataactct gtcgcataag gtcatttagc gaccaggaaa tcccggcggc atcataccct
     9541 tcatgcttct catcatcttc gccattccgc ccttcttcat tttcttcatc atgcgctgca
     9601 tgtcgtcgaa ctgtttcaga agacggttaa cgtcctgcac ctgcataccg caaccggcag
     9661 cgatacggcg tttacgcgaa cctttgatga tttctggctt agcgcgctct ttcatcgtca
     9721 tcgagttgat gatggcttcc atacgcacca gcactttatc gtccatctgc gacttgacgt
     9781 tatccgggat ctgccccatg cccggcagtt tgcccatcag gctggccatg ccgcccatat
     9841 ttttcatctg gcgcagttgc tcaaggaagt cattgagatc gaagccgtcg ccttttttca
     9901 gcttgctggc taatttctca gcttgcgcgc ggtcaacttt gctttcgata tcttcgatca
     9961 gcgacagtac gtcgcccatg ccgagaatac gggaagcgat gcggtccgga tggaacggct
    10021 ccagcgcctc ggttttctcg ccaacaccga ggaacttgat cggtttgccc gtaatgtgac
    10081 gaatagagag cgccgcaccg ccacgagcat cgccgtcgac tttagtcagt accacaccgg
    10141 tcagcggtaa cgcttcattg aatgcttttg ccgtattggc cgcatcctga ccagtcatgg
    10201 catcaaccac aaacagggtt tcaaccggat taatcgacgc atggacttgt ttgatctcgt
    10261 ccatcatcgc ttcgtcaacg tgcagacgac cggctgtatc caccagcagc acgtcgtaga
    10321 atttcagttt ggcttctttc agcgccgcgt taacgatatc taccggcttc tgaccaacat
    10381 cagaagggaa gaaatcaacg cccacctgct ctgccagcgt ctcaagctgt ttgattgccg
    10441 ccggacgata aacgtcggca gaaaccacca gcactttctt cttgtgcttc tcgcgcagga
    10501 acttaccgag tttaccaacg ctggttgttt taccggcacc ttgcaggccc gccatcagca
    10561 cgaccgcagg cggttgcgca gccaggttca gggtctggtt ctcttcgccc atcgccgcaa
    10621 ccagttcgtt acggacaatt ttgacgaact cctgccccgg cgtcaggctc ttattaactt
    10681 catgaccaac cgctttctct tttacgcgat tgataaactc acgcactacc ggcagagcga
    10741 cgtccgcctc cagcagcgcc atgcgcactt cgcgcagcgt atcttttacg ttgtcttcag
    10801 tgaggcgtcc acggccactg atattgcgca gcgtgcgcga caaacgatcg gttaaattat
    10861 caaacattgt ctctcgcctg gggtggaaac ggttggccgc aatcgcgaca catcatcagt
    10921 attttgccgc agtataacat gaaggcgtct ttgttgttat gcaacggttg gagcagcgtt
    10981 cacctgacgc tatactgctt ctctttctta ttgctcaaac tgtcgacatt actatgcccg
    11041 tttttgctct gctcgcgctt gtcgcctact ccgtcagtct tgcgctgatt gttcccggtc
    11101 tgctgcaaaa aaacggcggc tggcggcgca tggctattat ttctgcggtc attgcgctgg
    11161 tctgccacgc aatcgctctg gaagcccgca tcctgcccga cggtgatagc ggacaaaacc
    11221 tcagcctgct gaacgttggt tcattggtca gtttgatgat ctgtacggta atgaccattg
    11281 tggcttctcg caatcgtggc tggttgctgc tacccattgt ctatgccttt gcgcttatca
    11341 acctggcgct ggcgaccttc atgcccaatg aatacatcac ccatctggaa gcaacgcctg
    11401 ggatgctggt gcacattggc ttatcgctct tttcctatgc cacgctgatt atcgccgccc
    11461 tgtacgcgct gcaactggcg tggattgatt accaactgaa gaacaagaag ctggcgttta
    11521 accaggaaat gccgccattg atgagtatcg agcgtaaaat gttccacatc acgcagattg
    11581 gcgtggtgct gctaacgctc acgctttgca ctggcctgtt ctacatgcac aacttattta
    11641 gcatggaaaa tatcgacaag gccgtgctct ctatcgtggc gtggtttgtc tatattgtgc
    11701 tgctgtgggg acattatcat gaaggatggc gtggacgccg cgtcgtctgg tttaacgttg
    11761 cgggtgcagt cattctgaca ctggcctact tcggcagccg aattgtccag cagttaatca
    11821 gctaaaccca gaaaaggagt ttcccctgga acacatttct actactacgt tgatcattat
    11881 tctgatcatc atggtggtca tttcagccta tttttccggg tccgaaaccg gaatgatgac
    11941 cctcaaccgc tatcgtctgc gacatatggc gaaacagggt aatcgctcgg ccaaacgcgt
    12001 cgaaaaattg ctgcgtaagc cagaccgcct gattagcctg gtgttaatcg gcaataacct
    12061 ggtcaatatt cttgcctccg cgctaggcac tattgttggg atgcgtttgt acggcgatgc
    12121 gggcgtggca attgcgactg gtgtgctgac ttttgtcgtg ctggtttttg ctgaggtatt
    12181 gccgaaaacc attgccgcgc tgtacccgga aaaagtcgct tatccgagta gttttctgct
    12241 ggctccgctg caaattttga tgatgccgct ggtctggttg ctgaatgcta tcacccgtat
    12301 gctgatgcgc atgatgggta tcaaaaccga tatcgtggtt agcggctctt tgagcaaaga
    12361 agagttgcgc actatcgtgc acgaatcgcg ctcacaaatt tcccgtcgca atcaggatat
    12421 gctgctgtcg gtgctcgatc tggaaaaaat gaccgttgat gacatcatgg tgccgcgcag
    12481 tgaaattatc ggtattgata tcaacgatga ctggaaatcg attctgcgcc aactctccca
    12541 ctcacctcac gggcgcatcg tgctctaccg tgattcgctg gacgacgcca tcagtatgct
    12601 gcgtgtgcgt gaagcctggc gactcatgtc ggagaaaaaa gagttcacca aagaaaccat
    12661 gctacgcgcc gcggacgaga tctattttgt cccggaaggt acgccgctca gcacgcagtt
    12721 ggtgaagttt cagcgcaaca aaaagaaagt cggcctggtc gtcaacgagt atggagacat
    12781 tcaggggctg gtgacggttg aagatattct ggaagagatt gtcggcgatt tcactacctc
    12841 gatgtcgcca acacttgccg aagaggtcac gccgcaaaac gacggttcgg tgattatcga
    12901 tggcaccgcc aacgtgcgag aaatcaacaa agcctttaac tggcatctac ctgaagacga
    12961 tgcccgcacg gttaatggcg tcattctgga agcgctggaa gagatccctg tcgctggcac
    13021 ccgcgtgcgt attggcgagt acgatatcga tattctcgac gtacaggata atatgattaa
    13081 gcaggtaaaa gtttttcctg tgaaaccgct gcgcgagagt gtggcggagt aaaaaaacgg
    13141 cccggcattc gcatgctggg ccgtgattta ttacgaaagc agaaattaag cttttgcctt
    13201 cgctacagtc accatcgccg cacgaatcgt acgaccgttc agcgtataac ctttctgcat
    13261 aatgcccagt acgttacctg gcgcaacgtc atcagattcc accattgcga tggcctgatg
    13321 cacatttggg tccagtggga cgttggtttc ggcgatcact tcaacgccaa acttacgcac
    13381 aacatccagc atcgacttca gcgtcagctc aataccttca accatcgcgg acatatccgg
    13441 gttagcttta tcagccactt ccagcgcacg atccaggcta tcaatcaccg gcagcaattc
    13501 gttgatgaat ttctccagcg cgaatttgtg agctttttca atatccagtt cagtacgacg
    13561 acgcaggttt tccatttcgg cttttacgcg caaaatgccg tcacgttcac gggtctgggc
    13621 ttcagccagc tgagcttcga gattcgcaat tttttcatcg cgcggatcca cctgctcagc
    13681 agaagcttct ggctcaactg cctcaatctc ttcgtgctga tccatgataa tttcttccgg
    13741 ggcttgcccc tcaggcgttt tctgttcttt actactcatg aatttctccg cgtttttttc
    13801 gcattcatct cgctaacttc gcttattatg gggatcagtt tcagggtttc aagggaagca
    13861 ctcacattgt catcaatctt cgcaacaagg acctcggaaa aatgaataat catttcaagt
    13921 gtattggcat tgtgggacac ccacggcacc ccactgcact gacaacacat gaaatgctct
    13981 accgctggct gtgcacaaaa ggttacgagg tcatcgttga gcaacaaatc gctcacgaac
    14041 tgcaactgaa gaatgtgaaa actggcacgc tcgcggagat tgggcaactg gcagatctcg
    14101 cggtagtcgt tggtggcgac ggtaatatgc tgggcgcggc gcgcacactc gcccgttacg
    14161 atattaaagt tattggaatc aaccgtggca acctgggttt cctgactgac cttgaccccg
    14221 ataacgccca gcaacaatta gccgatgtgc tggaaggcca ctacatcagc gagaaacgtt
    14281 ttttgctgga agcgcaagtt tgtcagcaag attgccagaa acgcatcagc accgcgatta
    14341 acgaagtggt acttcatcct ggcaaagtgg cgcatatgat tgagttcgaa gtgtatatcg
    14401 acgagatctt tgcgttttcg cagcgttccg atggactgat tatttcgacg ccaacaggct
    14461 ccaccgcata ttccctctct gcgggcggtc ctattctgac cccctctctg gatgcgatta
    14521 ccctggtgcc catgttcccg catacgttgt cagcacgacc actggtgata aacagcagca
    14581 gcacgatccg tctgcgtttt tcgcatcgcc gtaacgacct ggaaatcagt tgcgacagcc
    14641 agatagcact gccgattcag gaaggtgaag atgtcctgat tcgtcgctgt gattaccatc
    14701 tgaatctgat tcatccgaaa gattacagtt atttcaatac attaagcacc aagctcggct
    14761 ggtcaaaaaa attattctaa ttttacgcca gcctctttac tgtatataaa accagtttat
    14821 actgtacaca ataacagtaa tggtttttca tacaggaaaa caactatgtt ggcacaactg
    14881 accatcagca actttgctat cgttcgtgag cttgagattg attttcatag cggcatgacc
    14941 gtaataactg gcgagaccgg cgcgggtaaa tctattgcaa tagatgccct cggcctttgt
    15001 ctcggtggtc gcgctgaagc cgacatggtg cgtaccggcg ctgctcgtgc cgacctgtgc
    15061 gcccgttttt ctctgaaaga tacgccagcg gccctgcgct ggctggaaga aaaccagctt
    15121 gaagacggtc atgaatgttt gcttcgtcgc gtaatcagta gcgatggtcg ctcacgaggt
    15181 tttatcaacg gtacagctgt tcctctgtca caacttcgcg aactgggcca gttgctgatt
    15241 cagatccatg gtcagcacgc ccatcagtta ctcaccaaac ccgagcacca aaaattcctg
    15301 cttgatggct atgccaatga aacctctctg ctccaggaaa tgaccgcacg ttatcagttg
    15361 tggcatcaaa gctgccgtga cctcgcgcat catcaacagt taagtcagga acgcgccgcc
    15421 cgtgcggaac tgctgcaata ccaattaaaa gaacttaacg aatttaatcc gcagcccgga
    15481 gagtttgagc aaatcgacga agagtacaaa catctggcga acagcggtca attgctgact
    15541 accagccaga atgcattggc agtaatggcc gacggtgaag acgcaaacct gcaaagtcag
    15601 ctttacacgg ctaaacaact ggtgagcgaa ttgattggca tggacagcaa actgtccggc
    15661 gtacttgata tgctggaaga agctaccatc cagattgctg aagccagcga tgaactgcgc
    15721 cactactgcg atcgtctgga tctcgatccc aaccgactgt ttgaacttga acagcgcatc
    15781 tcaaaacaga tttcgctggc acgtaaacat cacgtcagcc ctgaggcatt gccacagtat
    15841 taccagtcgc tactggaaga acagcagcaa ctggacgatc aggccgactc acaagaaacg
    15901 ctcgcgctgg cggtaacgaa acatcatcag caggcactgg aaaccgcgcg cgcattacac
    15961 cagcaacgcc agcagtatgc agaagaactt gcactgctga tcaccgacag tatgcatgcg
    16021 ctctcaatgc cgcatgggca gtttacgatc gatgttaaat ttgacgagca tcacctgggc
    16081 gctgacggtg ccgatcgtat tgagtttcgg gtaaccacca acccaggtca gccaatgcag
    16141 cctattgcca aagtcgcatc cggtggtgaa ttgtcacgca tcgcattggc aattcaggtc
    16201 atcacggcgc gtaaaatgga aaccccggca ctgatttttg atgaagtgga tgtagggatt
    16261 agcggtccaa cagcggcagt tgtcggcaaa ctgctgcgtc aacttggcga atcaactcag
    16321 gtgatgtgtg ttacccacct gccacaagtc gcgggatgtg gtcatcaaca ctattttgtc
    16381 agcaaagaaa ccgatggtgc gatgacagaa acacacatgc aatccctgga taaaaaagcg
    16441 cggttacaag agctggcgcg cctgcttggg ggcagtgaag tcacacgtaa cacactggcg
    16501 aatgcgaaag aactgcttgc ggcgtaaact tttttcctgc ttcacggtca gagtaaacag
    16561 caaaacgccg taagaccgga aagcaaaagg ttttaaagtg atgaaaggtc tattatcatc
    16621 ggcatattac agatgagcca cgtactgctc gggcccgaaa aggaatcaaa tcactatgcg
    16681 ctgtaaaacg ctgactgctg cagcagcagt actattgatg ttgaccgcag gctgttccac
    16741 tctggagcga gtggtttacc gtcctgacat caaccagggg aattatctga ccgctaacga
    16801 cgtatccaaa atacgcgttg gcatgacgca acaacaagtt gcgtacgcat tgggtacacc
    16861 gctgatgtcc gatccatttg gtacgaatac ctggttctat gtcttccgcc agcaaccagg
    16921 tcatgaaggt gtaacgcagc aaacgctgac gctgaccttt aatagtagcg gggtgttgac
    16981 caatattgat aataaacctg cgctgagtgg taactaatca taaagttgct ctcaaagacg
    17041 ttaaaaaagg tgctcaatga gcaccttttt tctgtctgtt atttattcgc tgatttttct
    17101 gctcgttgcc tgcgcagctc tttcggatcg gcaatgagag ggcgataaat ctccacccga
    17161 tcgccatcat gcacactatc gctcagtttt gccggacggc tataaatgcc gactttgttt
    17221 ttagttaaat cgatatcggt acgcaattcc agcaagccac tggcgcgaat agcttcttca
    17281 accgtcgcac cctcctgcag cgtcacacgc tgcaggtact gcttctcagg tagcgcataa
    17341 gccacctcaa cggcaatttt acctggcact gtaaacctct ttcgcacgaa ccgtaaaagc
    17401 ctggaccata ttagccgcca gttctttaaa cacgcggcca aaggcgagtt caatcaactt
    17461 attggtaaac tcaaaatcga gatgaaactc gatacggcac gcctcctgac tcagcggcgt
    17521 aaacttccat ccaccgatca atttcttgaa cggcccatcc accagattca taagaatact
    17581 ttggttactg gtcaactggt tgcgggtagt aaacgttttg ctgatcccag ccttagagac
    17641 atctaccgcc gcagtcatct gcccaggagt ggactccaga atccgacttc cggtacaacc
    17701 cggcaaaaac tgaggataag actgaacgtc attcactaac tgatacattt gctccgcgct
    17761 gtagggtacc agtgcggtcc ggctaatctg aggcataaca atttccatca acaaaaatcc
    17821 aacaaataat atcatttatc ctgctgtaaa aaaaacgcta tcccggcgct gggtaacatc
    17881 gggttcatgc taagatagag ccttgtcccc cgcaggattg atatggggtg ttttcgattt
    17941 cagattaccg atgattcacg acgcttatga cgaagaaaaa agcacataaa cctggttcag
    18001 cgaccatcgc gcttaacaag cgcgcccggc acgaatactt tatcgaagaa gagttcgagg
    18061 cgggacttgc cctgcaaggc tgggaggtta aatccctgcg tgcaggaaaa gccaatatca
    18121 gcgacagcta cgtccttctg cgtgacggag aggcatttct gtttggcgct aacatcacgc
    18181 caatggctgt ggcctccacg catgtggtgt gcgatcctac ccgtacccgc aagttacttc
    18241 tcaaccagcg cgaactggac tcattgtacg gtcgcgtcaa tcgagaaggc tataccgtag
    18301 tggcgctctc cctgtactgg aaaaatgcct ggtgcaaagt gaaaatcggc gtcgccaaag
    18361 gtaagaaaca gcatgataaa cgttcagata tcaaagaacg cgaatggcag gtggataaag
    18421 cgcgtatcat gaaaaacgcc caccgttaaa cctgcactgc aattattgat ccgctcctca
    18481 ccgcgcctcc ctctccggcg gcgcgaatgc acatcttatt ggctatcaca tccgacccaa
    18541 atgttgccat cccattgctt aatcgaataa aaatcaggct acatgggtgc taaatcttta
    18601 acgataacgc cattgaggct ggtcatggca ctcatgaatc tggtatactt accgttacac
    18661 attggggctg attctggatt cgacgggatt tgcgaaaccc aaggtgcatg ccgaggggcg
    18721 gttggcctcg taaaaagccg caaaaaatag tcgcaaacga cgaaaactac gctttagcag
    18781 cttaataacc tgcttagagc cctctctccc tagcctccgc tcttaggacg gggatcaaga
    18841 gaggtcaaac ccaaaagaga tcgcgtggaa gccctgcctg gggttgaagc gttaaaactt
    18901 aatcaggcta gtttgttagt ggcgtgtccg tccgcagctg gcaagcgaat gtaaagactg
    18961 actaagcatg tagtaccgag gacgtaggaa tttcggacgc gggttcaact cccgccagct
    19021 ccaccaatca tgattggacg gtgtaaggac aacaccaaca aaaacaggaa gttagcagtc
    19081 tcagcaagac accgaccaga cggtgaggag acataaaagg atacgcaaag gagccgcggc
    19141 tcctggtgat atgaaagccc acagatgtgg gctttttcgt tgatggtcag aacgaccagt
    19201 tcacaccagc caccgcgttc cacggggatt ccacaccggc accatggcta taccccaccc
    19261 cgagatgccc gcttaacgaa ctgctgaatg aggctttaat acctgcctgg tatattccac
    19321 gtctgcccga caaatcattg acaaaattac cgtcattatt cactttcacc cggttatcat
    19381 cgacaaattc tttgcgcaca gccgccttca gccacggctc aacttccatg ccgttcccca
    19441 gacgcatgtt gtaactcagc gttgcgccca gttcacgata tatactacgg gtatcgactg
    19501 atttcgattc catgccattg gataaatgat attcggggtt atcagcggtg aaccccgtta
    19561 acgatgcata cggcgtcagg ttccagttac catcggtaaa tcgcatcccg gtttcaatgt
    19621 gaccgcccag cccgttgctg cgataactgc cgttggcggc tccaccgctg ctcattttac
    19681 cggctacgtt actttcaaag cggttcagct tcacgatacc gtccagatag aaaccacttt
    19741 catgttccca gctggcatag ccccccagag aataactgcc cacactgcca tgccctccac
    19801 gatcaaaaaa gatatgtgta tgggaataac ccataaaagc gcccagcgtc gcaatcccct
    19861 caggaatatc attacggcta tcgatcccca ccgtcattcc ggtcagcgtc tgctcaaacc
    19921 cggcacccgc atcggtggtg acattattac gggtgttata cgtcgtcccc cagacattat
    19981 tgttgtgtgg actcgctttc attatgttca accgctcgcg aatactgttt agctcagcat
    20041 caaagaccaa cggtaatgtt gctgccatat tgagtacggc tgccgtagaa ggcgtgatgc
    20101 gtttctccgg gacgggtgtc ggcgttggat cgggagtcgg gtctggtttc ggatccggtt
    20161 ttgggtctgg ttttggatcc ggcttcggat ttgggttggg gtccgggttg ggtttgacat
    20221 cattggtcag gttccagttg ctgttgccat cgcttttcag gacatactca taggtcccaa
    20281 gatcaacgaa accgccggta ttacccagcg taaacgaagc atcccctccc cctgttttca
    20341 ccagcgtcat cgcgtcgtca gactgagggc tgacgccggt atcctgaaca aagattttaa
    20401 aattgccagt ggcgttgttg ttgacgacca gttgatcgcc ccgggagcct gcaacgttgg
    20461 tatgcaggta gaaattaccg ctgccggaaa gttcactggt ggtcagcgta ttgtagatac
    20521 cattagttgt ggcaggcgct gctgtttgtg ctgataaata aacatcaccg tcgttgagga
    20581 gcaaactatt tactctatat tcgcagttgc tggtgcctgc acaggaatta ttgctgttaa
    20641 gccagacact gccccggctg gcagccgtca ggtcggcaag cgtcgtatcg acaccatttc
    20701 ccagagttaa tgtcgcgcta tcggtaatcc ggaccgcccc ttcaagtttt actggcgtaa
    20761 cattatcccg tggcgtcatt aacgacaggc ttcctgtcgc gccactgacc gatgcatccg
    20821 ccagcgtacc tgcgtagacg attgccgtcc cgccagcaac ctgtagatct tctgcccggg
    20881 ccagaacctg aaactcatct tttgaccgac cgagggtata ttgcccacca gaattaacct
    20941 ttgtggcgaa gtcctgcccc aggttttgca ttgccccacc gttgccaacc gtggaatcgc
    21001 gagcttcggt acctgctaat accaataaat taccgccatt ttccagcagt acattggtcg
    21061 ctaaatatcc ggcaatggaa aaggtgccgt actggtgcgt accgctgatt tcaataccgt
    21121 tagccgtgct cgtctggaga gcggcaccgc tgttctggac gatatctgtc gctttgccat
    21181 tatcgttgac tgtcagcgtt ccgccttcat tgatcgttgt ttttattgcc tctccgttag
    21241 ctgaaactgt ttgtattccg ccgtcgttaa ttgttgtctc atttgccaca ccctcgacga
    21301 tttgttcacc gccagtgagc gtcgtgccag tcgcagtggc ttttgttttg acgatctccc
    21361 gaccgcccgc attgacctgt gttttggcgg aagtggtgtc tgactccacg gttaacacgc
    21421 cgccatttgt cagcaggata ttgttcgccg caccctgctc gatgctgaac gcgacgccat
    21481 cagcgcgtgt ccctgtgacc cgcgtcgccc tggtggttgc aaccaacgcg ccctgactac
    21541 tctgctgtat ccccgttgcg ctgcctttct cccgcacatc gagtgtgccg ccgtcattaa
    21601 gcaccgagtt ttcagccaga ccgccctcat taactacctg tgaaccgcca ttaataatgg
    21661 aaccttccgc tgtcccgttt gccataattt gttgtaagcc agagacgata tcggtattga
    21721 tcgccttacc ataattctga acggtttgtg tgccgccatt gatgtgcgtt ttgtctgttg
    21781 acccaccatc aacaatttgt tcaccacttt caatatttgc atcagtggct aaaccatata
    21841 ccgtttgctt gccacctttg atatttgctt tatcagaagt ggcactggca tatattgttt
    21901 gggtgcccgc actattgagt acagtgccaa catcttttcc ataaacatcc attttgccgt
    21961 tggcattaat aatcgtatca actgcccggg aaccagtgac gactgttaat gagccagcgt
    22021 tttccagcac tacattttta gcttctgaat tcctgatgta gaaagcatca ccataactgt
    22081 tggttccttc gataagggtt ccggaagtcg tggaagcaat taatgcgccg ccggattgtt
    22141 gctcaacatg cttagcctca ccaccgtcct gaacctccag aacgccacca ttattaagtc
    22201 tggtgctatc tgttttagcc tccttctgga caatcagctt accgccagca tcaatggtag
    22261 tatttttcgc cgaggtttta gccattaccg tcagttcgcc ggtattttcc agcacaacat
    22321 aattagcctc acctccggta atagtgaagt gagagagctt attgtatcct tcgatatcag
    22381 tccctgcgcc cgtgttggca actaaagcac tgcccgtctc ctggttaacc ccatgtgcaa
    22441 taccgccggt atagacaatc agcgagcctc cggcgctaat attgctgcca gttgccgtac
    22501 catctttctc aacaacctgc cggctcccag aggatatatt tgtcgtgtca gctttcccgc
    22561 cgctcttaat attttgtgtt ccgctgttga tattggtgcc cgtggcaata ccataattat
    22621 taatattctg tgtgccacca ttaattatgg tatttgtcgc gtttccggca acatccataa
    22681 cgccgccatt atctattcgg gtcgcatcag ctttagcatt cgttaaaact gacattgttc
    22741 ctttatcttt aataatcgtc ttatttgccg aaccatatgc gtttatgtct aaatgaccac
    22801 cgttttccag caacacattg tctgccacgt tattgtggat ggagaatgcc ccttcactat
    22861 tcgtaccgct caccgtcaaa tcgtaagtcg ttactttcaa agcggctcca tcgtgctgag
    22921 taacatttgt tgccatacca cctctaacat caagcacgcc accggaataa atttcaataa
    22981 catccgaggt gctagtgtaa tcaacaattt gcgtaccacc agaatagatc tgagtatttt
    23041 ttgccgttga cttactatcc agagactgag ttccaccttc aatcgtcgtg tccagcgcac
    23101 ggctctcata tactctttgc tcaccgccat ttttaatggt ggttgtttct actgtgctct
    23161 gttcaacata ctgtcgacca ccatttatgg ttgtgttcgt cgccagactt ccttgcgtga
    23221 tgtcctgata accggactta tttatcgttg taccatcaac atgcccctga gttgttactg
    23281 tctgccctcc accatcaatg atggttccat tcgcagtccc cccacttatg tgtgaattac
    23341 cacccttaat tgtcgttcca ttgctgatac cacctgtata aacgtcctga ttgccactct
    23401 cgattattgt acctgtggaa atacccccgt catgaattga ctgtctgccc ccgttaatgg
    23461 ttgtattatt agcctgcccc acaaaattgt tatgacctcc tacatcttga tatccacccg
    23521 attcaataag acttccatta gatatcccgc cataaacatt ctgctggcca tggttgataa
    23581 tatgagtgtt atttgttgta cctcgttcat ctactttttg gctgccatct acagtctcat
    23641 cgtttaccac accaataaca tcaggagtga aggccgccat cccgggcggg gcatatatca
    23701 aggcagatat caataaggaa agtactgagc gacgacaata atagggactg gtcctgttca
    23761 taaatttcat cctctgaaaa gtgaatactg agtagcgttt aagcgacctt agctttgctg
    23821 caacatcagc ccacaggcac cagaccaggg gattcatcct gaagagacag cgcaagtgta
    23881 ttgtgttcac cgctcatcaa agacatcatg atgaaatgat gatattccgc ataagaatga
    23941 ggcatttttt aaacgcagtg cgctgaagtg tggttggata aaaaagtcaa tctattcagg
    24001 aaacacgggc gtattctttt ctttcgacaa tcaggcagtc ggaaaaataa aatgatttac
    24061 ataatcgttt ctgatgaata tcttctgctc acataaaaat cacacaataa ctctgagatc
    24121 gcagattgtt ttacttttac agcttccgtc cccccattgt tgggcaaata tagattgggc
    24181 cagagcacga aagttaatac cacgttcgca cagctcctcc aacagcacga caagatgcca
    24241 catactgcgc cccagtcggt tcagtttaca gactagcagt gcgtccccta ccgataatgt
    24301 cctgagccgt ttgttctgtc cgggcctgct gccacggtta tgcgctgaag tggtaactct
    24361 gagcacgcag ttcttcagtc aggcgtggtg caccgtcacc ctattattgc tgggtaagat
    24421 caaaaaactt tcaagcagct aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc
    24481 aattacagca ccagttcaga caccgccaac tcatcaaata aatcaagggg ttacgtgaaa
    24541 gcgtagcccc ttttactttg gtagtgacag caaaatggat gcagtgtgaa gaataatccc
    24601 gtttactcaa tcaataatac atattatttc aatctacgtt attatttctt tgtaaaaatc
    24661 gccatttatt tatcattgaa aactgctttt agaactttat acaaggggac tagtcacaac
    24721 aggactattc tcaacgggct catcctcaga ggaactatca tcaaagtcat catccataga
    24781 tgaaatacca tcgaatggtg ccacgcccgt gattagtttt attttattat tacgatcagt
    24841 caagactcca tttaaaccat tttcgctgac ggattttaat aatttatcat aactcttatt
    24901 gtcatcaggc acattaaaaa tacacggagt atcaacatca aacaatgacg ttccccagtt
    24961 cacatattta atatcatagt tactgaagtt ctatccagaa aagaagcatc ccttaaaatc
    25021 caatccacct aaattatata aaccaccctc ttctttttgg agagcaatgt taattttggc
    25081 tatctcccgg acatcatcgc catttttgta tttgaatacc gtttcaagat gttctccaga
    25141 cagcttgact tctggaaata atttgaaatt aaaccctaca ttattatgtg tcaacgtaga
    25201 tgaacaaaag atggaaaaag cttgcagtgc tgaattatag ctatcgattt tatcttgagg
    25261 ctgcgctctt ggtaaaaact tatagcaaca ctcataaaaa ttaagtagaa actctgatga
    25321 ccttccattt ttatcaaata atacccgctg aaaatgctgc acaaacttat tcgttttttc
    25381 ttgaatatct acggtgtgtt cctttgactc tgacaaagat gaagcctgtt gaagtgcatt
    25441 gctcatctgg ttaatgaaca tgtgcatttc tttaaattta tctccatagt gacctttaac
    25501 ttgctgcgag tttttagcta caggtgaaat cttatctatt tggttgtcat ataaattatg
    25561 tgattccata cagtcatttg gcggcgtttc tgaaataaca ctacgtgttc t
//
LOCUS       DAZKGF010000065        25384 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZKGF010000065 DAZKGF010000000
VERSION     DAZKGF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.065"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(204..1016)
                     /locus_tag="ACHOQV_002451"
     CDS             complement(204..1016)
                     /locus_tag="ACHOQV_002451"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFP9630814.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRN"
     gene            1278..2042
                     /gene="udp"
                     /locus_tag="ACHOQV_002452"
     CDS             1278..2042
                     /gene="udp"
                     /locus_tag="ACHOQV_002452"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFP9630815.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(2076..3566)
                     /locus_tag="ACHOQV_002453"
     CDS             complement(2076..3566)
                     /locus_tag="ACHOQV_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HFP9630816.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(3579..4085)
                     /locus_tag="ACHOQV_002454"
     CDS             complement(3579..4085)
                     /locus_tag="ACHOQV_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HFP9630817.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(4102..5076)
                     /locus_tag="ACHOQV_002455"
     CDS             complement(4102..5076)
                     /locus_tag="ACHOQV_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HFP9630818.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASYPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPNGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMAYSKANPGKVRIGN
                     SGTGAIWHLAVAALEDKTGTKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVNG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            complement(5101..5730)
                     /locus_tag="ACHOQV_002456"
     CDS             complement(5101..5730)
                     /locus_tag="ACHOQV_002456"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFP9630819.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYRPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(5720..6724)
                     /locus_tag="ACHOQV_002457"
     CDS             complement(5720..6724)
                     /locus_tag="ACHOQV_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFP9630820.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQSAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEHGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(6748..7515)
                     /locus_tag="ACHOQV_002458"
     CDS             complement(6748..7515)
                     /locus_tag="ACHOQV_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP9630821.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            7732..9159
                     /gene="rmuC"
                     /locus_tag="ACHOQV_002459"
     CDS             7732..9159
                     /gene="rmuC"
                     /locus_tag="ACHOQV_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFP9630822.1"
                     /translation="MDFSIIVYAIIALVSVVIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            9254..10009
                     /gene="ubiE"
                     /locus_tag="ACHOQV_002460"
     CDS             9254..10009
                     /gene="ubiE"
                     /locus_tag="ACHOQV_002460"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFP9630823.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            10023..10628
                     /gene="ubiJ"
                     /locus_tag="ACHOQV_002461"
     CDS             10023..10628
                     /gene="ubiJ"
                     /locus_tag="ACHOQV_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFP9630824.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            10625..12265
                     /gene="ubiB"
                     /locus_tag="ACHOQV_002462"
     CDS             10625..12265
                     /gene="ubiB"
                     /locus_tag="ACHOQV_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFP9630825.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            12344..12613
                     /gene="tatA"
                     /locus_tag="ACHOQV_002463"
     CDS             12344..12613
                     /gene="tatA"
                     /locus_tag="ACHOQV_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFP9630826.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            12617..13132
                     /gene="tatB"
                     /locus_tag="ACHOQV_002464"
     CDS             12617..13132
                     /gene="tatB"
                     /locus_tag="ACHOQV_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFP9630827.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            13135..13911
                     /gene="tatC"
                     /locus_tag="ACHOQV_002465"
     CDS             13135..13911
                     /gene="tatC"
                     /locus_tag="ACHOQV_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFP9630828.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            13953..14735
                     /gene="tatD"
                     /locus_tag="ACHOQV_002466"
     CDS             13953..14735
                     /gene="tatD"
                     /locus_tag="ACHOQV_002466"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFP9630829.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKVPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(14732..15220)
                     /gene="rfaH"
                     /locus_tag="ACHOQV_002467"
     CDS             complement(14732..15220)
                     /gene="rfaH"
                     /locus_tag="ACHOQV_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFP9630830.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            15387..16880
                     /gene="ubiD"
                     /locus_tag="ACHOQV_002468"
     CDS             15387..16880
                     /gene="ubiD"
                     /locus_tag="ACHOQV_002468"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFP9630831.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            16926..17627
                     /gene="fre"
                     /locus_tag="ACHOQV_002469"
     CDS             16926..17627
                     /gene="fre"
                     /locus_tag="ACHOQV_002469"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFP9630832.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(17819..18982)
                     /gene="fadA"
                     /locus_tag="ACHOQV_002470"
     CDS             complement(17819..18982)
                     /gene="fadA"
                     /locus_tag="ACHOQV_002470"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFP9630833.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMIGDAQTCLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLTQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISSTLLNLMERKDAQFGLATMCIGLGQGIATVFERI"
     gene            complement(18992..21181)
                     /gene="fadB"
                     /locus_tag="ACHOQV_002471"
     CDS             complement(18992..21181)
                     /gene="fadB"
                     /locus_tag="ACHOQV_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFP9630834.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYSGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFNANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKMA"
     gene            21371..22702
                     /gene="pepQ"
                     /locus_tag="ACHOQV_002472"
     CDS             21371..22702
                     /gene="pepQ"
                     /locus_tag="ACHOQV_002472"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFP9630835.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENITRDLKLA"
     gene            22702..23316
                     /locus_tag="ACHOQV_002473"
     CDS             22702..23316
                     /locus_tag="ACHOQV_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFP9630836.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            23355..24806
                     /gene="trkH"
                     /locus_tag="ACHOQV_002474"
     CDS             23355..24806
                     /gene="trkH"
                     /locus_tag="ACHOQV_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFP9630837.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            24818..25363
                     /gene="hemG"
                     /locus_tag="ACHOQV_002475"
     CDS             24818..25363
                     /gene="hemG"
                     /locus_tag="ACHOQV_002475"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFP9630838.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         5986 a         6500 c         6852 g         6046 t
ORIGIN      
        1 tttgagaatg ggagttgatg gtaaatctct aaggtgtctg ccagtgaaga gactcattta
       61 tgactatgac ttgccgactt tcttaaggtc aaatagcgga gtcgtgctct ccaatcaatt
      121 catactgatt attttttggg ggacagagat ggcagcaaaa tgcactcgtt cactcaccat
      181 ctcaaatata gactaagagg aaactaattc cgcttcccac catactgctt aaaccactcc
      241 agcatacgct gccagccatc tttcgcagat tcggcatgat agctcgggcg ataatcggcg
      301 ttgaatgcat gcccggcatc cgggtagacg ataatctctg ctttcgcatt agcagcccgc
      361 agcgcctggc gcatggtttc aacgctctcc tgcggaatgc tgttatcctg accaccatat
      421 aagccgagaa ccggcgcgtt aagatcggtt gcgatatcaa caggttgttt cggtgaattc
      481 agcgacttgt cgcccgtcag tttgccgtac cacgccactg cggcttttag ctgtggatta
      541 tgcgcggcat acagccaggt gatacgtcca ccccagcaga atccggtgat cattaaacga
      601 tgaacatcgc cgccgttgcg ggaagcccaa ctggcgacat gatcgagatc ggccagcacc
      661 tgcgagtcag gcacttttgc taccagaccg ctaagcaacg tggggatatc ggcaaaatca
      721 ttcggatcgc cttcgcggaa gtaaagttca ggtgcaatag ccagataccc ctccagcgcc
      781 agacggcgac aaatgtcgcg gatatgttca tgcacgccaa aaatttcctg cactacaatg
      841 accactggca gtgggccatc gctttgcttt ggtctggcat ggtaagcagg catgttatcc
      901 ccttgtgaag ggatagaggt gaagcccgcc acaatcgcgt cgtccggagt ctgaacgatg
      961 gtcgaagcga gaggcgatgc cgcaggtgca aatccagatt gttgtgttgt tgccatggta
     1021 ttctccgtac ccttataaaa atgttgcgca atgttaacta tagtcagcat gcaacaaatc
     1081 acattgcctg aatcggctca tcttttatgc agtcctgcag aatgaagagt gatttatgtg
     1141 atttgcatca cttttgatgg gtaaatttat gcaacgcatt tgcgtcatgg tgatgagtat
     1201 cacgaaaaaa tgttaaaccc ttcggtaaag tgtctttttg cttcttctga ctaaaccgat
     1261 tcacagagga gttgtatatg tccaagtctg atgtttttca tctcggcctc actaaaaacg
     1321 atttacaagg ggctacgctt gccatcgtcc ctggcgaccc ggatcgtgtg gaaaagatcg
     1381 ccgcgctgat ggataagccg gttaagctgg catctcaccg cgaattcacc acctggcgtg
     1441 cagagctgga tggtaaacct gttatcgtct gctctaccgg tatcggcggc ccgtctacct
     1501 ctattgctgt tgaagagctg gcacagctgg gcattcgcac cttcctgcgt atcggtacaa
     1561 cgggcgctat tcagccgcat attaatgtgg gtgatgtcct ggttaccacg gcgtctgttc
     1621 gtctggacgg cgcgagcctg cacttcgcac cgctggaatt cccggctgtc gctgatttcg
     1681 aatgtacgac tgcgctggtt gaagcggcga agtcaattgg tgccaccact cacgttggtg
     1741 tgacggcttc ttctgatacc ttctacccgg gtcaggaacg ttacgatact tactctggtc
     1801 gcgtagttcg tcactttaaa ggttctatgg aagagtggca ggcgatgggc gtaatgaact
     1861 atgaaatgga atctgcaacc ctgctgacca tgtgtgcaag tcagggcctg cgtgccggta
     1921 tggtagcggg ggttatcgtt aaccgcaccc agcaagagat cccgaatgct gagacgatga
     1981 aacaaaccga gagccatgcg gtgaaaatcg tggtggaagc ggcgcgtcgt ctgctgaaat
     2041 aaaaaacgct ccccaacttc ggggagcgat atcagttact gacgccgtaa acgcattatt
     2101 ttgcggatta ttggcgtcag tagcgtaaag gctgccagag ttagcaatgc cagagtgatc
     2161 ggtcgggtat acagaaagtc cagtgaaccc cctgaaagcg acagcgcacg gcgaaaatta
     2221 ctttctgcca tcgggcccat aatcaatgcc agcactacgg gtgatgccgg atatcctcct
     2281 ttctgcatga aatagcccac cacgcctgca atcagcataa tgccaacatc aaacaggcta
     2341 ttgttcaacg cataagtccc gaccacacaa agtgcgagga tcatcggtgt gagaatattg
     2401 ccggggatct tcaccacgtt accgatgact ttcaaagaga gcagtccaag aactagcagc
     2461 atgaagtaac aaaagatcat gccgacaaac agtgtataaa ccatatcgcc gtggtcggta
     2521 aacatcagcg ggcctggttg cattccctgt aacgttaacg cgcccagcat gatggcggtt
     2581 accgcatcgc cagggatccc cagcgtcagc ataggtaaca atgcgccacc ggtgcagcca
     2641 ttcgcgcctg cctcacagga ggccacagct ttaatttcac ccttaccaaa gttttctggt
     2701 gtcttactga aacgacgagt ttcgttatag gcaacaaaag ctgcaatatc cgcccccgca
     2761 ccggggatca tgccgataaa aacccctaat cctgatgagc gcaaaatggt taatgcaatg
     2821 cggcgtagtg tttgccaggg cagtaacaga ctgccgatag ccaccttcgc cccctgacga
     2881 atttgctggt tttgctccat agagcggaat gcttctgaag cagcaaataa accaatcatc
     2941 accgggataa atggcacatt catcagctcc acaaatccac ctgtaaaccg tggaaaaccg
     3001 cccattggat caaggcctat cgttgcgatc agcaatccgc caaccccggc aatcagccct
     3061 ttaatcagcg actcaccgga aatactggca ataatactca gtccgaaggt tgccagggcg
     3121 aaggactccg aggcactaaa tttcagcgcc caactggcaa gtaccggaga gagaaacatc
     3181 agcacgataa tgctgagcgt tccgccacta aaggaagaga acgtggcggc actcaatgcc
     3241 agccctgctt ttccctgttt cgtcagctca taaccatcaa tagcggtggc tgcggcggca
     3301 ggcgttccgg gaattttgag caaaattgcg gtgatagaac caccgtagac gccaccaaaa
     3361 aagacgccgc aaatcatcaa caagccagaa acaggctcca tgccgtaggt gaaaggcagc
     3421 aaaatcgcca cacccatggt cgctgtcaga ccaggcaaag cgccaatcac aatacccagc
     3481 atcaccccaa aagtggcaaa cagcagcgcc tgaggattat tgagtaatgt cgaaaaaccc
     3541 tgagttaaaa gttcactttc aaacattgct ggcccctgtt attcgaaaaa tgtgccgaca
     3601 ggcaatggca cctgtaacgc ttcgacaaac agcaaccaga ggattgccgt taacagcagc
     3661 gcaatacccg gtgtgagtac ccatcgcctg tagcccatca acgtcataat gacgatgagg
     3721 aaaacaggag tagtaatgag ataaccgcag tagctcatcg ccacaatata aaacgcggtt
     3781 gccgcaattc ctgttgctgt tttcctgaat gccggttttt cctgtgcgtt ggcgcaagag
     3841 gctggttttg cctgcaaaag atcgcgtatc accagcaaga cagcgagcac aatcagggca
     3901 caggcataga aggtagggaa ggcacttgca ccgggatccg tttcaatggc agttgctgga
     3961 aaatcagcgg cattaatcat caccgcgcca gcaaccacac aaagtaacag cgccaccacc
     4021 atcgttgatg aaaggcgctt gcggtgcggt acggcgagtt gagcaacaga ctgttttgtg
     4081 ttcatcaacc gtacctcagg tttatttttt caggcccagg cgagtcagca actcgtcaaa
     4141 gtacttttcc tgttcgctga tttgggtctg gaagctggca gcgtcaagcc atgcatagtt
     4201 gagattcaac ttttgcagtg catcctggaa tgcaggctct tcagccgtct cttttgctgc
     4261 ctttgccaga acatccacca catcctgcgg cgttttttgc gacacaatca ggccgcgcca
     4321 ggtgccgatg gagagatcaa cgcctttctc ttttaacgtc gggacgtcag gcatggtttt
     4381 cattcgctca tccgccatca ctaccagtgt cttcagcttg ccgccattca catggttgat
     4441 aacttctcct gggcttacgg aaaccgcttc aatatgcccg cctaacaggc ctgtaatggc
     4501 aggggctgcg ccgtcatacg ggacatgaga aaactttgtg cccgttttgt cttccagtgc
     4561 agctaccgcc agatgccaga tagctccggt gcctgagtta ccaatgcgta cttttccggg
     4621 attcgctttg gagtaagcca taaattcttc atagctattc cacggcgcat cggcacggac
     4681 tgtgatagca gccggatccg cattcagacg ggcaatgggt ttaaagtcgc tggttttaaa
     4741 acgcaccatt ccgaggctgg gaagggtggt cagttcaacc gtgcctaaac caattttgta
     4801 accgttaggg cgggcagcgg cgatttcact caggccgata gcaccgcctc cgccaggttt
     4861 attgataacc ccgatgctga cgggtaaatg gtttttggcg gcatcagcaa aggcacgggc
     4921 aaccagatcc gtaccgcctc cggcagcgta gggaacgact aactcgatct gtttggttgg
     4981 gtaggaggca gcctgtacag gcgcaatgct gagggcagcg cctgcaagaa gtaccattgc
     5041 agccattttt ttcggtaagg taagatgttt tatcattgct tttatatcct tatttatcag
     5101 ttagttgcta tttaaattgt tccatgcgtt cataaacagt actgcatttt catagacttg
     5161 ctctggcgtc atgccggggc gatatagcgc agaaccgagg ccaaagccgt ttgcgccgtg
     5221 gctcagataa cgcgccatgg tttccggtgt gataccgcca accggtaaca tcggaacgga
     5281 ttgtggaata accgcacgcc aggctttggt aacttctggc gtaatttgtt cagccgggaa
     5341 cagcttgatg gcattagcac catgttcaat ggcagcaaag gcttcggtgg gcgtggcgac
     5401 gccaggcgca caatacatac ccagcgcacg ggcgcgacgg atcaccgcag tatcgcaatg
     5461 cggcatcacg ataatctggc ccccagcctc tttaaccgca accacctgct ctaccgaaag
     5521 cacagttccg gctccgacca ggcagtcctt aggcagggcg tcgcgcagta aactgatact
     5581 ttgcaacgcc tccggcgagt tcataggaat ctcaatcagg cggaatccgg cggcgtacag
     5641 tttttcaccg atggcgacaa tctcatccgg tttcacgccg cgtaaaatgg cgactaaccc
     5701 ggttttttgc aaaagtgtat taaatggcat gggtacattc cctggcgttg gtggacagta
     5761 acccggcagc gtgggcgatt cgccataatc catgttcagt ggcattagga atggcgtgta
     5821 tctgtctgtc gggaaaaacg tggctgaatg cttgtgaata gcggctgttc agatttgcgc
     5881 taccgattaa ggcgatcggt gttgtggcgg gtaaatcacg tagcaaactg gatgccagac
     5941 cacataattc atggcctatc agtaatccgg aaaggtaatc cccctgcgag gacgacgtaa
     6001 gattactggt cagcaggcgc gaacgggtcg aaaacagcgt cgataataat ccggtctgat
     6061 gatgctcttg tgctgctgtc agcccgtggg tgaaggcatc ccagtgcggt tcatctccag
     6121 gatgcatggt ggcaccgaga atcgaatggc gggataaaac atcaaataac tcgcccgtca
     6181 taaaggttcg gaaatcggta atggtgttgt tttcaactac tgcccatttc gcatgtgtgc
     6241 cgggtaagcc tatcagcact ggcataccct gatggatcgc gttttgcagg gccggttcca
     6301 tcgagatagc accgaagatt tgtgtctctt cgccacgcat cacttcgggc atttcacctt
     6361 ctttaatcac gccaggaatg atgtgcagca ttgtctgctg ctggtggata acaggcgtca
     6421 actgcttcgc cagaccctcg agagtgaccg ggcaaggtaa ataagctgct gattgccacc
     6481 cttgcgcact gccgagcatg ccgcaagcca gtaccgggca tggggtttgt gccagccagt
     6541 cgccgcagac acgtaaaaac gtatcgtgga aagcgtcctc acgtgttgct gcctgggagg
     6601 gtaatttcat gatcccccag gggaattcac gtttttcttg tggatttggt gattcgccaa
     6661 aacgccatgc ccgaagcgat gacgttcccc agtccagggc aataaatgag ggtgctcccg
     6721 gcattagtcg tttcctttaa tcatggttta tttatctttg cggacgtgct taccccagcc
     6781 cagttcagcg gatatctctt ctgcacagga tttgacgtaa ggtgccagtt cttccagacg
     6841 cgccagcgac atatacgtgg tggtactggc gacagaaata gccgccacaa tttcatcccg
     6901 ggcgttgtaa accggcgctg caacgcaacg gatagtcggc tcgttctctt ccagatcaaa
     6961 agcaaaacca ctggcggcat aacgacgcat gttctgaata aatgcgccca gttttgtttc
     7021 atcaccacag gtcttaaaca gcgtgcgcca ctcttcttct gttcggttga ggatcaatgc
     7081 cttgccgatg ccggtgattg ccagtggcat gcgatggcct gggcgtgagc gcatttccag
     7141 cccgcgctga ctgttgatct tctccagata cagcacttcg tcgccttcaa cgatccccag
     7201 atgcacggtg tccagggtgt aacgggcaag gcgttgcaaa acgggctggg caatttcgta
     7261 cagtgaggtg ctttccagtg ctctggcacc gaattcgatc agtttggcac ccagttggta
     7321 gccctgcgtt gggacatggc gcaggtagcg ttgttccacc agaacggtga ctaaacgatg
     7381 ggttgtgctc cgggtcgtgc cggtgaactc gccgatactt tttaggtcat ggcagccgtt
     7441 ataaacagca ttaagaaccg ccagtccacg taataacgtt tgtgcgcctg cggtggttga
     7501 agaggtttct gccatacgtt tctcctgatg atattgctga agtgttccac ataatgacgt
     7561 ggggaaaagg tgatatatcc cacaatataa actccatctc atattgtggg ataaaataat
     7621 gaaaattaac gatcgttttg cgtagcgcct cgcaggaaac ttctttcaaa ctggacgttt
     7681 gtacagcaca attctatttt gtgcgggtaa gttgttgggt caggaggctt tgtggatttc
     7741 tcaatcattg tttatgccat tattgcgctg gttagtgtgg tcattggctg gctgtttgcc
     7801 agttatcaac atgcgcagca aaaagccgag caattagctg aacgtgaaga gatggtcgcg
     7861 gagttaagcg cggcaaaaca acaaattact caaagcgagc actggcgcgc tgagtgcgag
     7921 ttactcaata acgaagtgcg cagcctgcaa agtattaaca cctctctgga ggccgatctg
     7981 cgtgaagtaa ccacgcggat ggaagccgca cagcaacatg ctgacgataa aattcgccag
     8041 atgatcaaca gcgagcagcg cctcagtgag cagtttgaaa acctcgccaa ccgtattttt
     8101 gagcacagca atcgccgggt tgatgagcaa aaccgtcaga gtctgaacag cctgttgtcg
     8161 ccgctacgtg aacaactgga cggtttccgc cgtcaggttc aggacagctt cggtaaagaa
     8221 gcacaggaac gtcataccct gacccacgaa attcgcaatc tccagcagct caacgcgcaa
     8281 atggctcagg aggcgatcaa cctgacgcgc gcgctgaaag gcgacaataa aacccagggc
     8341 aactggggcg aggtggtatt gacgcgggtg ctggaggctt ccggtctgcg tgaagggtat
     8401 gaatatgaaa ctcaggtcag catcgaaaat gacgcccgct cgcggatgca gccggatgtc
     8461 atcgtgcggt tgccgcaggg aaaagatgtg gtgatcgacg ccaaaatgac gctggtcgcc
     8521 tatgaacgct atttcaacgc cgaagatgac tacacccgcg aaagcgcgct acaggaacat
     8581 atcgcgtcgg tgcgtaacca tatccgtttg ctgggacgca aagattatca acagctgccg
     8641 gggctgcgaa ccctggatta cgtgctgatg tttattcccg ttgaacccgc ttttttactg
     8701 gcgcttgacc gccagccgga gctgatcacc gaagcgttga aaaacaatat catgctggtt
     8761 agcccgacta cgctgctggt ggcgctgcgc actatcgcca acctgtggcg ttatgagcat
     8821 caaagccgca acgcccagca aatcgccgat cgtgccagca agctgtacga caagatgcgt
     8881 ttgttcgtcg atgacatgtc agcgattggt caaagtctcg acaaagcgca ggataactac
     8941 cgccaggcaa tgaaaaaact ctcttcaggt cgcggaaatg tgctggcgca ggcagaagcg
     9001 tttcgcggtt taggggtaga aattaaacgc gagattaatc cggatttggc tgaacaggcg
     9061 gtgagccagg atgaagagta tcgacttcgg tcggttccgg agcagccgaa tgatgaagct
     9121 tatcaacgcg atgatgaata taatcagcag tcgcgctagc ccattggggg tagttaagcc
     9181 gggtagaaat ctagggcatc gacgcccaat ctgttacact tctggaacaa ttttttgatg
     9241 agcaggcatt gagatggtgg ataagtcaca agaaacgacg cactttggtt ttcagaccgt
     9301 cgcgaaggaa caaaaagcgg atatggtcgc ccacgttttc cattccgtgg catcaaaata
     9361 cgatgtcatg aatgatttga tgtcattcgg tattcatcgt ttgtggaagc gattcacgat
     9421 tgattgcagc ggcgtacgcc gtgggcagac cgtgctggat ctggctggtg gcaccggcga
     9481 cctgacagcg aaattctctc gcctggtcgg agaaactggc aaagtggtcc ttgctgatat
     9541 caatgaatcc atgctcaaaa tgggccgcga gaagctgcgt aatatcggtg tgattggcaa
     9601 cgttgagtat gttcaggcga acgctgaggc gctgccgttc ccggataaca cctttgattg
     9661 catcaccatt tcgtttggtc tgcgtaacgt caccgacaaa gataaagcac tgcgttcaat
     9721 gtatcgcgtg ctgaaacccg gcggccgcct gctggtgctt gagttctcga agccaattat
     9781 cgagccgctg agcaaagcct atgatgcata ctccttccat gtgctgccgc gtattggctc
     9841 actggtcgcg aacgacgccg acagctaccg ttatctggca gaatccatcc gtatgcatcc
     9901 cgatcaggat accctgaaaa ccatgatgca ggatgccgga ttcgaaagtg tcgactacta
     9961 caatctgacg gcaggggttg tggcgctgca tcgtggttat aagttctgac aggagaccgg
    10021 aaatgccttt taaaccttta gtgacggcag gaattgaaag tctgctcaac accttcctgt
    10081 atcgctcacc cgcgctgaaa acggcccgct cgcgtctgtt gggcaaagtg ctgcgcgtgg
    10141 aggtaaaagg cttttcgacg tcattgattc tggtgttcag cgaacgccag gttgatgtac
    10201 tgggcgaatg ggcaggcgat gctgactgca ccgttatcgc ctacgccagt gtgttgccga
    10261 aacttcgcga tcgccagcag cttgccgcac tgattcgcag tggtgagctg gaagtgcagg
    10321 gcgatattca ggtggtgcaa aacttcgttg cgctggcaga tctggctgag ttcgaccctg
    10381 cggaactgct ggccccttat accggtgata tcgccgctga aggtatcagc aaagccctgc
    10441 gcgggggcgc aaagttcctg catcacggca ttaagcgcca gcaacgttat gtggcggaag
    10501 ccattactga agagtggcgt atggcacccg gtccgcttga agtggcctgg tttgcggaag
    10561 agacggctgc cgtcgagcgt gctgttgatg ccctgaccaa acggctggaa aaactggagg
    10621 ctaaatgacg ccaggtgaag tacggcgcct atatttcatc attcgcactt ttttaagcta
    10681 cggacttgat gaactgatcc ccaaaatgcg tatcaccctg ccgctacggc tatggcgata
    10741 ctcattattc tggatgccaa atcggcataa agacaaacct ttaggtgagc gactacgact
    10801 ggccctgcaa gaactgggac cggtatggat caagttcggg cagatgttat caacccgccg
    10861 cgatcttttt ccgccgcata ttgccgatca gctggcgtta ttgcaggaca aagtcgctcc
    10921 gtttgatggc aagctggcga agcagcagat tgaagctgca atgggcggct tgccggtaga
    10981 agcgtggttt gacgattttg aaatcaagcc gctggcttct gcttctatcg cccaggttca
    11041 taccgcgcga ttgaaatcga atggtaaaga ggtggtgatt aaagtcatcc gcccggatat
    11101 tttgccggtc attaaagcgg atctgaaact tatctaccgt ctggctcgct gggtgccgcg
    11161 tttgctgccg gatggtcgcc gtctgcgccc aaccgaagtg gtgcgcgagt acgaaaagac
    11221 cttgattgat gaactgaatt tgctgcggga atctgccaac gccattcagc ttcgacgcaa
    11281 ttttgaagac agcccgatgc tctacatccc ggaagtttac cctgactatt gtagtgaagg
    11341 gatgatggtg atggagcgta tttacggcat tccggtgtct gatgttgcga cgctggagaa
    11401 aaacggcacc aacatgaaat tgctggcgga acgcggcgtg caggtgttct tcactcaggt
    11461 ctttcgcgac agctttttcc atgccgatat gcaccctggc aacatctttg taagctatga
    11521 acacccagaa aacccgaaat atatcggcat tgattgcggg attgttggct cgctaaacaa
    11581 agaagataaa cgctatctgg cggaaaactt tatcgctttc tttaatcgcg attatcgcaa
    11641 agttgcagag ctacacgtcg attcaggctg ggtgccgcca gataccaacg ttgaagagtt
    11701 cgaatttgcc attcgtacgg tctgtgaacc tatctttgag aaaccgttgg ccgaaatttc
    11761 gtttggacat gtgctgttaa atctatttaa tacggcgcgt cgcttcaata tggaagtgca
    11821 gccgcaactg gtgttactcc agaaaaccct gctctacgtc gaaggggtag gacgccagct
    11881 ttatccgcag ctcgatttat ggaaaacggc gaagcctttc cttgagtcgt ggattaaaga
    11941 tcaggtcggt attcctgcgc tggtgagagc atttaaagaa aaagcgccgt tctgggtcga
    12001 aaaaatgcca gaactgcctg aactggttta cgacagtttg cgccagggca agtatttaca
    12061 gcacagtgtt gataagattg cccgcgagct tcagtcaaat catgtacgtc agggacaatc
    12121 gcgttatttt ctcggaattg gcgctacgtt agtattaagt ggcacattct tgttggtcag
    12181 ccgacctgaa tgggggctga tgcccggctg gttaatggca ggtggtctga tcgcctggtt
    12241 tgtcggttgg cgcaaaacac gctgattttt tcatcgctca aggcgggccg tgtaacgtat
    12301 aatgcggcct tgtttaatca tcatctacca cagaggaaca tgtatgggtg gtatcagtat
    12361 ttggcagtta ttgattattg ccgtcatcgt tgtactgctt tttggcacca aaaagctcgg
    12421 ctccatcggt tccgatcttg gtgcgtcgat caaaggcttt aaaaaagcaa tgagcgatga
    12481 tgaaccaaag caggataaaa ccagtcagga tgctgatttt actgcgaaaa ctatcgccga
    12541 taagcaggcg gatacgaatc aggaacaggc taaaacagaa gacgcgaagc gccacgataa
    12601 agagcaggtg taatccgtgt ttgatatcgg ttttagcgaa ctgctattgg tattcatcat
    12661 cggcctcgtc gttctggggc cgcaacgact gcctgtggcg gtaaaaacgg tcgcgggctg
    12721 gattcgcgcg ttgcgttcac tggcgacaac ggtgcagaac gaactgaccc aggagttaaa
    12781 actccaggag tttcaggaca gtctgaaaaa ggttgaaaag gcgagcctca ctaacctgac
    12841 gcccgaactg aaagcgtcga tggatgaact acgccaggcc gcggagtcga tgaagcgttc
    12901 ctacgttgca aacgatcctg aaaaggcgag cgatgaagcg cacaccatcc acaacccggt
    12961 ggtgaaagac aatgaagctg cgcatgaagg cgtaacgcct gctgctgcac aaacgcaagc
    13021 cagttcgccg gaacagaagc cagaaaccac gccagagccg gtggtaaaac ctgctgcgga
    13081 cgctgaaccg aaaaccgctg caccttcccc ttcgtcgagt gataaaccgt aaacatgtct
    13141 gtagaagata ctcaaccgct tatcacgcat ctgattgagc tgcgtaagcg tctgctgaac
    13201 tgcattatcg cggtgatcgt gatattcctg tgtctggtct atttcgccaa tgacatctat
    13261 cacctggtat ccgcgccatt gatcaagcag ttgccgcaag gttcaacgat gatcgccacc
    13321 gacgtggcct cgccgttctt tacgccgatc aagctgacct ttatggtgtc gctgattctg
    13381 tcagcgccgg tgattctcta tcaggtgtgg gcgtttatcg ccccagcgct gtataagcat
    13441 gaacgtcgcc tggtggtgcc gctgctggtt tccagctctc tgttgtttta tatcggcatg
    13501 gcgttcgcct actttgtggt ctttccgctg gcatttggct tccttgccaa taccgcgccg
    13561 gaaggggtac aggtatccac cgacatcgcc agctatttaa gcttcgttat ggcgctgttt
    13621 atggcgtttg gtgtctcctt tgaagtgccg gtggcaatag tgctgctgtg ctggatggga
    13681 attacctcgc cagaagactt acgcaaaaaa cgtccgtatg tgctggttgg cgcatttgtt
    13741 gtcgggatgc tgctgacgcc gccggatgtc ttctcgcaaa cgctgctggc gatcccgatg
    13801 tattgcctgt ttgaaatcgg tgtcttcttc tcacgctttt acgttggtaa agggcgaaat
    13861 cgggaagagg aaaacgacgc tgaagcagaa agcgaaaaaa ctgaagaata aattcaaccg
    13921 cccgtcaggg cggttgtcat atggagtaca ggatgtttga tatcggcgtt aatttgacca
    13981 gttcgcaatt tgcgaaagac cgtgatgatg ttgtagcgcg cgcttttgac gcgggagtta
    14041 atgggctact catcaccggc actaacctgc gtgaaagcca gcaggcgcaa aagctggcgc
    14101 gtcagtattc gtcctgttgg tcaacggcgg gcgtacatcc tcacgacagc agccagtggc
    14161 aagctgcgac tgaagaagcg attattgagc tggccgcgca gccagaagtg gtggcgattg
    14221 gtgaatgtgg tctcgacttt aaccgcaact tttcgacgcc ggaagaacag gaacgcgctt
    14281 ttgttgccca gctacgcatt gccgcagaat taaacatgcc ggtatttatg cactgtcgcg
    14341 atgcccacga gcggtttatg acattgctgg agccgtggct ggataaagtg cctggtgcgg
    14401 ttcttcattg ctttaccggc acacgcgaag agatgcaggc gtgcgtggcg catggaattt
    14461 atatcggcat taccggttgg gtttgcgatg aacgacgcgg gctggagctg cgggaattgt
    14521 tgccgttgat tccggcggag aaattgctga tcgaaactga tgcgccgtat ctgctccctc
    14581 gcgatctcac gccaaagcca tcatcccggc gcaacgagcc agcccatctg ccccatattt
    14641 tgcaacgtat tgcgcactgg cgtggagaag atgccgcatg gctggctgcc accacggatg
    14701 ctaatgtcaa aacactgttt gggattgcgt tttagagttt gcggaactcg gtgttcttca
    14761 cactgtgctt aatctcttta ttaataagat tcagcaatag catggagcga gcctcgccgt
    14821 ctggctcggt gaaaatggcc tgaaaacctt cgaatgcgcc ttcagtaata atcactttat
    14881 caccaggata cggcgtcgat ggatcgacaa tatctttcgg tttatacact gataactgat
    14941 gaatcaccgc cgatgggact atcgctggcg acgcgccaaa gcgcacgaag tggctgacac
    15001 cgcgggtcgc gttgatagtc gtggtatgaa tcacttccgg atcgaactcc acaaacaagt
    15061 agttggggaa caatggctca ctgactgcag tacgtttgcc acgcacgatt ttttccaggg
    15121 tgatcatcgg tgccaggcaa ttcacagcct gtctttcgag gtgttcctgg gcacgttgaa
    15181 gttgcccgcg cttgcagtac agtaaatacc aggattgcat aatgactctt atccgtttaa
    15241 tcggggcgca aggatagcaa aagctttacg ctaagttaat tatattcccc ggtttgcgtt
    15301 ataccgtcag agttcacgct aatttaacaa atttacagca tcgcaaagat gaacgccgta
    15361 taatgggcgc agattaagag gctacaatgg acgccatgaa atataacgat ttacgcgact
    15421 tcttgacgct gcttgaacag cagggtgagc taaaacgtat cacgctcccg gtggatccgc
    15481 acctggaaat cactgaaatt gctgaccgca ctttgcgtgc cggtgggcct gcgctgttgt
    15541 tcgaaaaccc taaaggctac tcaatgccgg tgctgtgcaa cctgttcggt acgccaaagc
    15601 gcgtggcgat gggcatgggg caggaagatg tttcggcgct gcgtgaagtt ggtaaattat
    15661 tggcgtttct gaaagagccg gagccgccaa aaggtttccg cgacctgttt gataaactgc
    15721 cgcagtttaa gcaagtattg aacatgccga caaagcggct gcgtggtgcg ccttgccaac
    15781 aaaaaatcgt ctctggcgat gacgtcgatc tcaatcgtat tcccattatg acctgctggc
    15841 cggaagatgc cgcgccgctg attacctggg ggctcaccgt aacgcgcggc ccgcataaag
    15901 agcggcagaa tctgggcatt tatcgccagc agctaattgg taaaaacaaa ctgattatgc
    15961 gctggctgtc gcatcgcggc ggcgcgttgg attatcagga gtggtgtgcg gcgcatccgg
    16021 gcgaacgttt cccggtttct gtggcgctgg gtgccgatcc cgccacgatt ctcggtgcag
    16081 tcacccccgt tccggatacg ctttcagagt atgcgtttgc cggattgctg cgcggcacca
    16141 agaccgaagt ggtgaagtgt atctccaatg accttgaagt gcccgccagt gcggagattg
    16201 tgctggaagg gtatatcgaa caaggcgaaa ctgcgccgga agggccgtat ggcgaccaca
    16261 ccggttacta taacgaagtc gatagttttc cggtatttac cgtgacgcat attacccagc
    16321 gtgaagatgc gatttatcat tccacctata ccgggcgtcc gccagatgaa cctgcggtac
    16381 tgggtgtagc actgaacgaa gtgttcgtgc cgattctgca aaaacagttc ccggaaattg
    16441 tcgattttta tctgccgccg gaaggctgtt cttatcgtct ggcggtagtg acgatcaaaa
    16501 aacagtatgc cggacacgcg aagcgcgtca tgatgggcgt ctggtcgttc ttacgccagt
    16561 ttatgtacac taaatttgtg atcgtttgcg atgatgacgt caacgcccgc gactggaacg
    16621 atgtgatttg ggcgattacc acccgtatgg acccggcgcg ggataccgtt ctggtagaaa
    16681 atacgcctat tgattatctg gattttgcct cccctgtctc cgggctgggt tcaaaaatgg
    16741 ggctggatgc cacgaataaa tggccggggg aaacccagcg tgaatgggga cgtcccatca
    16801 aaaaagatcc agatgttgtc gcgcatattg acgccatttg ggatgaactg gctattttta
    16861 acaacggtaa aagcgcctga tgcgcgtttg ttttgcccta tttatcgatc cgacagagaa
    16921 agcgcatgac aaccttaagc tgtaaagtga cctcggtaga agctatcacg gataccgtat
    16981 atcgtgtccg catcgtgcca gacgcggcct tttcttttcg tgctggtcag tatttgatgg
    17041 tagtgatgga tgagcgcgac aaacgtccgt tctcaatggc ttcgacgcca gatgaaaaag
    17101 ggtttatcga gctgcatatt ggcgcttctg aaatcaacct ttacgcgaaa gcagtcatgg
    17161 accgcatcct caaagatcat caaatcgtgg tcgatattcc ccacggagaa gcgtggctgc
    17221 gcgatgatga agagcgtccg atgattttga ttgcgggcgg caccgggttc tcttatgccc
    17281 gctcgatttt gctgacagcg ttggcgcgta acccaaaccg tgatatcacc atttactggg
    17341 gcgggcgtga agagcagcat ctgtatgatc tctgcgagct tgaggcgctt tcgttgaagc
    17401 atcctggtct acaagtggtg ccggtggttg aacaaccgga agcgggctgg cgtgggcgta
    17461 ctggcaccgt gttaacggcg gtattgcagg atcatggtac gctggcagag catgatatct
    17521 atattgccgg acgttttgag atggcgaaaa ttgcccgtga cctgttttgc agcgagcgta
    17581 atgcgcggga agatcgcctg tttggcgatg cgtttgcatt tatctgagat ataaaaaacc
    17641 cgcccctgac aggcgggaag aacggcaact aaaactttat acagtggcat ttagatctat
    17701 gtcgcatccg gcattcggca cacgctgcct gatgcgacgc tggcgcgtct tatcaggcct
    17761 acaattggtg caggatcggt agaccggata aggcgttcac gccgcatctg gcaagtggtt
    17821 aaatccgctc aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgcaagac
    17881 caaactgggc atccttgcgc tccatgagat ttagcagcgt gctgctgata cgcgcaccgg
    17941 aacagcccaa cggatgaccc agcgcgatcg cgccaccgtt gaggttgatc ttctcgtcaa
    18001 tctgctcaat cagtcccaga tctttaatac atggcaggat ctgcgcggca aaggcttcat
    18061 tcatttcaaa cacgccgata tcactgacag aaagccccgc ttttttcagc gccaattttg
    18121 aggccggaac cggaccgtaa cccataatcg acggatcaca accaacgacc gccatcgaac
    18181 gcacacgggc gcgcggctta agacctaatt catgggcgcg gctttcactc atcaccagca
    18241 tggcagctgc gccatcggaa agcgcagaag atgtacccgc cgttaccgta ccgctgacgg
    18301 gatcaaacgc cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt
    18361 cgtcgtaatt aaactgcgtc aggacgccgt cggcatcgtg accaccggtc gggataattt
    18421 catttttaaa tgcacccgac tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa
    18481 aggcatcctg catttcacgg ctgataccgt gcatacgcgc cagcatttct gccgttaagc
    18541 ccatcatgcc cgccgctttg gcgacattac ggctaaggcc ggggtgaaaa tcgacgccgt
    18601 gactcattgg cacatggccc atatgctcca cgccgccaac cagacatgtc tgcgcatcgc
    18661 cgatcatgat cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat
    18721 tgacagtaac cgccgggaca gagtgcggca cttctgccaa cagcgccgcg ttacgggcga
    18781 tattaaaacc ctgctccagc gtctgctgta cacaacccca gtaaatatcg tcgagggccg
    18841 ccgcttccag cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt
    18901 ccgcacgcac gtgacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat
    18961 cgacaatgac aacctgttcc attgtgactc cttaagccat tttcaggtcg ccaaccggac
    19021 gggctggctc aaccggggga tagtacggtt cgttatgacg cgctttatta cgcagacctt
    19081 ccggcacttc atacagcggg ccgagatgct gatattgctg tgccatatcg aggtattttg
    19141 cgctaccgag ggtgtccagc cagcggaacg cgccgccgtg aaacggaggg aagcccaggc
    19201 cgtagaccag cgccatatcc gcttctgccg gagtggcgat aatgccttct tccagacagc
    19261 gcaccacttc gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat
    19321 cgcgcttcgg ctggctcact tctgccagca ggtcgtcaac gacggcatct tcttctttct
    19381 tcggcttacc tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa
    19441 agcggttggc attaaacagc gcgtcgatgg catcgcgata atctttctgc atccgctgcg
    19501 ggaagcctgc tgccatgaca gcctgggcgt gatgcgcggt gtcaatgccc acaacatcca
    19561 gcagatatgc cggacccatc ggccagccaa actgtttttc catcactttg tcgatcttgc
    19621 ggaaatccgc gccgtcgcgc agcaactggc tgaaaccggc gaaatagggg aacagtacac
    19681 ggttgacgaa gaagccgggg cagtcgttaa ccacgatcgg cgtcttgccc atcttgctcg
    19741 cccatgcgac gactttcgcg atggtttcgt cggagctttt ctcgccgcga ataatttcca
    19801 ccaacggcat ccggtgcacc gggttaaaga agtgcatccc gcagaagttt tccggacgtt
    19861 ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat
    19921 ccgggcgtac tttctgctcg gtttctgcca gtacggcttt tttcactttc gggttttcga
    19981 caaccgcttc taccacaaca tccacgcggt caaaaccaga gtaatcgagc gttgggtgga
    20041 ttgtggagat cacgccagcc agtttcagac catcgatctt gccgcgctca agctgcttgt
    20101 tcagcagttt cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca
    20161 tgacaaccgg tacgcctttc cacgcagact ggtaagcgat gccgccaccc ataatgcctg
    20221 cgcccagcac agcggcctgt ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc
    20281 cttttacata ttgatcgtta aggaaaatgc cgaccagtgc gcgagcttcg ttggtatgcg
    20341 ccagcgggac aaaacttttg ttttccagat ttaatgcttc ttcacgacca aaacgggccg
    20401 ccgcttcaat ggtttttact gcggtgatgg gggccggata atgtttcccc gccgtttgtg
    20461 cgaccatccc tttagcgatg gtgaaactca tggtggcttc aatcttgctc agttttagcg
    20521 gttccagctt cggctgacgt ttcgctttcc agtcgaggtc gccgttaatg gcctggcgta
    20581 aaatcgccat cgccccttca accagttttt ctgctttgac tacaccatcc accagaccga
    20641 ttttcagcgc ctgatccgcg ccgacatctt taccggcggc aatgatttcc agcgcactgt
    20701 cagcgcccag catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt
    20761 tggtttccgg cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca
    20821 cgcattcgca gccaccgccc agcgcatagc cgttgacggc agcaatggtc ggcaccggga
    20881 gatcttccag gcgattaaac acgctattgg caaagtgcag ccactgactt aactgttctt
    20941 caggaacgag gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt
    21001 tgttcgaacg cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct
    21061 cgccgaggct ggcgacggtg gcagtgtcga gtttattaac tgaacctggg gcatcaaata
    21121 ccagttcggc aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca
    21181 tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca gatgcatcga agtgtgtatt
    21241 ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc gatctgatca cgctcggcag
    21301 aaatcacgct ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga
    21361 agggtaaaaa atggaatcac tggcctcgct ctataaaaat catatagcta ccttacagga
    21421 acggactcgc gatgcgctgg cgcgcttcaa gctggatgcg ttacttattc actccggcga
    21481 actgttcaac gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa
    21541 agcgtgggtg ccagtaactc aggtgccaaa ctgctggctg ctggtggatg gcgtgaataa
    21601 gccgaaactg tggttctatc taccggttga ttactggcac aacgtcgaac cgctgccgaa
    21661 ctccttctgg actgaagatg tagaagtgat cgcgctgccg aaagccgatg gcattggtag
    21721 tctgttgcct gctgcgcgcg gcaatatcgg ttatatcggt ccggtgccag aacgtgcgct
    21781 gcaactgggt attgaggcca gcaatatcaa cccgaaaggg gtgatcgact acctgcatta
    21841 ctatcgctcc ttcaaaaccg agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc
    21901 ggtcaacggt catcgcgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat
    21961 caacattgcc tatctgaccg cgaccggtca tcgtgatacc gacgtacctt atagcaacat
    22021 tgtggcgctt aacgaacacg ctgcggtgtt gcattacacc aaactggacc accaggcacc
    22081 ggaagagatg cgcagcttcc tgctggatgc cggggccgaa tataacggct atgcggctga
    22141 tctaacccgt acctggtcgg caaaaagcga caacgactac gcacagctgg tgaaagacgt
    22201 aaatgatgaa caactggcgc tgatcgctac catgaaagca ggcgtcagct atgtggatta
    22261 ccatatccag ttccatcagc gcatcgccaa actgctgcgt aaacatcaaa tcatcaccga
    22321 tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat
    22381 cggccatccg ctgggcctgc aggtgcatga cgtagccggt tttatgcagg atgatagcgg
    22441 tacgcacctc gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc
    22501 gggcatggtg ttaaccatcg aaccgggtat ctacttcatc gaatcgcttc tggcaccgtg
    22561 gcgtgaaggg cagttcagca aacacttcaa ctggcagaaa attgaagcac tgaaaccgtt
    22621 cggcggcatt cgtatcgaag acaacgtggt gattcacgaa aacaacgtgg aaaacattac
    22681 ccgggatctg aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc
    22741 gttgaagaga tcaaaaagag ccgcttcatt acgctgttgg cgcataccga tggcgttgag
    22801 gcggcaaaag cgtttgttga atcggtgcgg gcagagcacc ccgatgcccg ccaccattgc
    22861 gtggcgtggg tggcgggtgc gccggatgat tctcaacagc tgggtttctc tgacgacggg
    22921 gagccggcgg gaacggcagg taaaccgatg ctcgcccaac taatgggcag cggcgtcggg
    22981 gaaattaccg ctgtggtggt gcgctactac ggcggcatat tgcttggcac cggtgggtta
    23041 gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag
    23101 acaccattaa ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg
    23161 ttgctggggc agtgtgacgg caaaattatt aacagtgatt atcaggcatt cgttctgctg
    23221 cgggtggcgc ttccggcggc gaaagtggct gaattttccg caaagctggc ggattttagc
    23281 cgtggttcat tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta
    23341 aggaagcggc agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct
    23401 tattttcagg gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg
    23461 gccgcgcttt tacccagacc ttttttgtcg ccctcgccat tggctctatg ctgtggtggc
    23521 cgaaccgcaa agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt
    23581 tctggaccgt gctgggtagc gtcggtgcgc tcccttttat cttctcggaa agcccgaacc
    23641 tcacgattac cgatgcgttt tttgaatctt tctctggcct gaccaccacg ggggccacta
    23701 cgctagtggg gctggattcg ctccctcacg ccatcctctt ttatcgccag atgctgcaat
    23761 ggtttggcgg gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg
    23821 gtgggatgca gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc
    23881 cgcgaattgc ggaaacggcg aaaaccctgt ggttgattta tgtcttgctg accgtcgcct
    23941 gtgcgctggc gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg
    24001 cgactatcgc tattggcggc ttctcgacac atgatgccag tatcggttat ttcgacagcc
    24061 cgactattaa cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc
    24121 acttttcact gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca
    24181 tgtttatcgg cgtacagttt acgctggtgg ttatttgtac actcgtactg tggtttcata
    24241 atgtctacag ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtatcgatgg
    24301 cgacaaccgc cgggtttacg actgacagca ttgcccgctg gccgctcttt ttgccggtac
    24361 tgctcttatg ttcagctttt atcggcggtt gcgccgggtc aacgggcggt ggcctgaaag
    24421 tgatccgcat cctgctgctg tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc
    24481 cgaacgccgt ctatagcatt aagctgggga atcgcgcact gccggaacgt atcctcgaag
    24541 ccgtgtgggg atttttctcc gcctatgcat tggtgtttat tgtcagtatg ctggcgatta
    24601 tcgccacggg cgtggatgac ttttctgcct ttgcctcggt tgttgcgaca ttgaataacc
    24661 tggggccagg gcttggtgtg gttgctgata actttaccag tatgaacccg gtggctaaat
    24721 ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgctct
    24781 ttaccccgac tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc
    24841 aacaagggac ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact
    24901 ggggatccag gcggatgtcg ccaatgtgca ccgcattgaa gaaccacagt gggaaaacta
    24961 cgaccgtgtg gtcattggtg cttctattcg ctatggtcac taccactctg cgttccagga
    25021 gtttgtcaaa aagcatgcga cgcgactgaa ttcgatgccg agcgcctttt actccgtaaa
    25081 cctggtggcg cgtaaaccgg agaagcgtac tccacagacc aacagctacg cgcggaagtt
    25141 tctgatgagt tcacaatggc gtcccgatcg ctgtgccgtc attgccgggg cgctgcgtta
    25201 cccacgttat cgctggtacg accgttttat gatcaagctg attatgaaga tgtcaggcgg
    25261 tgaaacggat acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc
    25321 ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa taagcataaa gaataaaaaa
    25381 tgtg
//
LOCUS       DAZKGF010000066        25224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZKGF010000066 DAZKGF010000000
VERSION     DAZKGF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.066"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(34..729)
                     /locus_tag="ACHOQV_002476"
     CDS             complement(34..729)
                     /locus_tag="ACHOQV_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP9630839.1"
                     /translation="MDGFSTERNNKGMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLDEIGVVFKYLGIDNVSFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERWVSTTKKASDCAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNDSCSVVG
                     KVIASQWPEETFS"
     gene            805..1020
                     /locus_tag="ACHOQV_002477"
     CDS             805..1020
                     /locus_tag="ACHOQV_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9630840.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVECLNKLGAKVSLDDVFPPEHKAA"
     gene            1140..1433
                     /locus_tag="ACHOQV_002478"
     CDS             1140..1433
                     /locus_tag="ACHOQV_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFP9630841.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            1466..1612
                     /locus_tag="ACHOQV_002479"
     CDS             1466..1612
                     /locus_tag="ACHOQV_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HFP9630842.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            1605..2438
                     /locus_tag="ACHOQV_002480"
     CDS             1605..2438
                     /locus_tag="ACHOQV_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HFP9630843.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     AAHLWVHLILKAKHTPEYVMTDAGEILVGRGKLLGGRNSLAFETGLKPDRVQYLLRKF
                     KKLGMIDWVSHGKFSVFSVEKYDDYQSNFVPADYQQITTDKEYNNIISNTDVLESTAA
                     DKKSDKKKPSVSCQDVVDAYHEILPEAPRIRALNDKRKNQIRTFWRKAGVITRQLDGH
                     GFTVQDWRNYLSYVGENCRWMFEERPNHQRGTVWHKKGFDFLLNDNTYLKVREGEHDD
                     R"
     gene            2428..3864
                     /locus_tag="ACHOQV_002481"
     CDS             2428..3864
                     /locus_tag="ACHOQV_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFP9630844.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKILFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTAMQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDMLYIDDTPGLSLTQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPGETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            4247..4495
                     /locus_tag="ACHOQV_002482"
     CDS             4247..4495
                     /locus_tag="ACHOQV_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012987735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630845.1"
                     /translation="MTYPHILEIHEKAAAINAGRKPQFGDKMRNLVAGEDNPRRDAYF
                     VKQKRVTGRMNPGLWYTMTERKGNFWDSCPKGLIFIDE"
     gene            4507..4797
                     /locus_tag="ACHOQV_002483"
     CDS             4507..4797
                     /locus_tag="ACHOQV_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630846.1"
                     /translation="MDESRKQFEESWLRRGGESSYLIRYPENHHEIGSGDIGGQYVMD
                     DVQGHWQTWQASRAAIEIELPESFTMHSGRTPYLYVSEVQSAIRAAGVKVKE"
     gene            4800..4964
                     /locus_tag="ACHOQV_002484"
     CDS             4800..4964
                     /locus_tag="ACHOQV_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630847.1"
                     /translation="MSAYEEIMLALRFFFGVEEDENVNEIIGQDHDPIGTIAAALDDY
                     RSVNGEETNV"
     gene            4945..5385
                     /locus_tag="ACHOQV_002485"
     CDS             4945..5385
                     /locus_tag="ACHOQV_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFP9630848.1"
                     /translation="MRKLTFELRSTIHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            5382..5558
                     /locus_tag="ACHOQV_002486"
     CDS             5382..5558
                     /locus_tag="ACHOQV_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9630849.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKQIPKESDVKTFNYTA
                     HLWDIRWLRERARK"
     gene            5561..5962
                     /locus_tag="ACHOQV_002487"
     CDS             5561..5962
                     /locus_tag="ACHOQV_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630850.1"
                     /translation="MLLIQPRFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANTTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            5922..6131
                     /locus_tag="ACHOQV_002488"
     CDS             5922..6131
                     /locus_tag="ACHOQV_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFP9630851.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCADCCAELMSDPNSSMYEEEDDG"
     gene            6124..6735
                     /locus_tag="ACHOQV_002489"
     CDS             6124..6735
                     /locus_tag="ACHOQV_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9630852.1"
                     /translation="MAKPARRRCKNNECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIG
                     QEAVDEIESNHNRHRWTIEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            6732..6938
                     /locus_tag="ACHOQV_002490"
     CDS             6732..6938
                     /locus_tag="ACHOQV_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFP9630853.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            6916..7587
                     /locus_tag="ACHOQV_002491"
     CDS             6916..7587
                     /locus_tag="ACHOQV_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFP9630854.1"
                     /translation="MRYYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLADRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFSNQMYIDTGAVFCGNLTLIQVQG
                     EGAWE"
     gene            7578..8096
                     /locus_tag="ACHOQV_002492"
     CDS             7578..8096
                     /locus_tag="ACHOQV_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9630855.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMCIDKKAFGILLSYYAHGSSKRAIASYYH
                     ATAKPRKMCGRGGEGWRKPSLATCRNEIDDILKASLFVLYQPMQNAFKMRKRVEKVKH
                     VAVKSLDMQLSI"
     gene            8160..8234
                     /locus_tag="ACHOQV_002493"
     tRNA            8160..8234
                     /locus_tag="ACHOQV_002493"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8193..8195,aa:Asn,seq:gtt)
     gene            8240..8315
                     /locus_tag="ACHOQV_002494"
     tRNA            8240..8315
                     /locus_tag="ACHOQV_002494"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8273..8275,aa:Thr,seq:tgt)
     gene            8558..8881
                     /locus_tag="ACHOQV_002495"
     CDS             8558..8881
                     /locus_tag="ACHOQV_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFP9630856.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            8865..9341
                     /locus_tag="ACHOQV_002496"
     CDS             8865..9341
                     /locus_tag="ACHOQV_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP9630857.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADNLIAKFKESGGTVREIEV"
     gene            9338..9805
                     /locus_tag="ACHOQV_002497"
     CDS             9338..9805
                     /locus_tag="ACHOQV_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9630858.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCSVPS
                     SAETSGASGMGNDSTVELSPVAGRNVLGVRDGIIRDQTALRTLQEYIRTQCLR"
     gene            9793..9945
                     /locus_tag="ACHOQV_002498"
     CDS             9793..9945
                     /locus_tag="ACHOQV_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630859.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGGHFWSKSRLNISLLMLDIEHLA
                     RGKGLR"
     gene            10151..10681
                     /locus_tag="ACHOQV_002499"
     CDS             10151..10681
                     /locus_tag="ACHOQV_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP9630860.1"
                     /translation="MKYPTVIVNGVSVRVDEDGRYNLNDLHAAAVANGEATEQQRPSQ
                     FLRSAQIKRFIKALEAKVQKSTLEQIQPLKIIKGGAEPGVWGVELLAIRYAAWIKPEF
                     EIEVYEVFKTVVRLGVGAMSRLNRIDHIINTETKAISQCASQMAKWGVGGRKRLLHVA
                     RERAANEVQMYLPGMV"
     gene            10938..11180
                     /locus_tag="ACHOQV_002500"
     CDS             10938..11180
                     /locus_tag="ACHOQV_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFP9630861.1"
                     /translation="MAEITPMTEEQKFQIEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDTTTRTIEAVRKSKEALDLFTTGA"
     gene            11183..11623
                     /locus_tag="ACHOQV_002501"
     CDS             11183..11623
                     /locus_tag="ACHOQV_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP9630862.1"
                     /translation="MARPTKYQEAYAEQARKLCLLGYTDAELADFFEVSESTINKWKL
                     DYPEFSESIKKGKAVADAEVSDRLYQRAMGFVAPDIDIRVIENRIVETPLEKYYPPDT
                     TAAIFWLKNRQKDKWRDKVDHELTGKDGGAIQIETSPMSTLFGK"
     gene            11620..13035
                     /locus_tag="ACHOQV_002502"
     CDS             11620..13035
                     /locus_tag="ACHOQV_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFP9630863.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKC
                     EWLEAETDAHKKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDAP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPESAAQPDYSSYSIPCGVG"
     gene            13037..15235
                     /locus_tag="ACHOQV_002503"
     CDS             13037..15235
                     /locus_tag="ACHOQV_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP9630864.1"
                     /translation="MAEKKMTDWHRKVLCNFDNAWSATQDMREQIIEAQRFVRVSGAQ
                     WEGSTNAGYSFDEGRFEHYPRFELNKIARECDRIIGEYRQNRISVKFRPKDDKASEAL
                     AEKMNGKFRADYQETSGGEACDNAFDDAVTGGFGCFRMCADYEDEMDPSNEQRRISLL
                     PVYDPATCVFFDQDSKQYDRSDAMWAMEMFSMTPKAFEAEYPDSIAASLSRDDTGTQY
                     DWSTPDAIYVGRYYEVRIEKVKLAAWRNPVSGETAIYDEEQIKDIVDELTDGAFELIG
                     ERTVKKRRVYCGLLSGAEWLEEPKRIPGEHIPLIPVYGRRSFVDNQERIEGHAAKAMD
                     AQRLENLMVSMIADNATQAGGDGIPIVDVDFIPGPLMNHWAERNKKRPAVLPMTSKKD
                     KNGTVISEAQVAGWTPPTQMPPALAGLLQYTGTAIQQITGASQLENMPSNVATDTVDS
                     IFNRMDTQSYIYMDNMAKSMRRAGVVWLSMAREVYGSDTPMRIVNEDGSDDVALMTGE
                     VVDRQTGQVIALNDLSQGNYEVTVDVGQSFATRRDATVKSLLSMLALIPPGTPKHDLV
                     SSMILDNMDGEGMDDLKEYNRNQLLLSGVIKPRTPEEQQMVEQAKQQQASQPDPAMVA
                     AQGQLLAGQAELQKAQNEQAAIQVKAFQAQTDAQVAAANVVKILASADSQQKSDIREA
                     LKLLGQFQQQQGDNARADAELVLKSQAQGHAQRMDISSILQKSTQQQPQQ"
     gene            15326..16219
                     /locus_tag="ACHOQV_002504"
     CDS             15326..16219
                     /locus_tag="ACHOQV_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFP9630865.1"
                     /translation="MDQMAENTPEVEIETDASEQIPDDVELAEEVETEDGGESSGNDA
                     EEATETDDDESEQEFYFGDEKLDSPTSEDSAEHGLVKHLRKTIKEKDRELKELMRQSQ
                     KPVEQQPVITQPPRMPKLDDEDIGFDEEIYQQRMAKWAEDNGKYQQQEMARKQKEQEL
                     QAAYQERLSKYQQRVKALKVPGYQEAEQAVLEEIPIETQNAILFESEKPEIVVLALGR
                     NAELRKQLAEATNPVAIGRLLERIESKARIMPKAKTTAATTPTVKGSNGAVINNLDKL
                     KAKALETGDWTPYFAAKKAKK"
     gene            16238..17491
                     /locus_tag="ACHOQV_002505"
     CDS             16238..17491
                     /locus_tag="ACHOQV_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFP9630866.1"
                     /translation="MANQLAKDLEIMFENYVEGFEAACVVSRNAKKFRPGDTAMQRAG
                     DVLYRPQHYHMNIEEGLDLSGKTPTALVQRLVPSVFKEPKNILYTLDAREMRDPEHKT
                     EAGRAAGMRLAAQIDSDLISMVTQRATNVITMADSTTGSQGRDLWNCAAGIDATMTAI
                     GVPQGINRRSFWNPFNYKDLAGELGHRAYAQGATLTAYEKAQIPPVASFDSYKTDISG
                     RVPKGTATSITLAAAPAHKVEAKDANDMPVDNRQGTITVSAEGLQVGDAFTIAGVNSV
                     HQITKDTTGQPQVFRVLAVSGTTVTISPKILPPDNADVASRPYANVDANAASSAAITI
                     LNKNAAPANLFWADGSVELMYGKLAFPTGQGPQVMTATTEQGATLIMSYAFDHIKGVT
                     TARFTTLYGCSVLVPEYTGIVIAGQ"
     gene            17533..17721
                     /locus_tag="ACHOQV_002506"
     CDS             17533..17721
                     /locus_tag="ACHOQV_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630867.1"
                     /translation="MARTMLYKPGNMITCGQFAVDYIIVDDEEVKSHLKKGWVKTPEE
                     TATKQKVAKAEEDGENEG"
     gene            17702..18163
                     /locus_tag="ACHOQV_002507"
     CDS             17702..18163
                     /locus_tag="ACHOQV_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFP9630868.1"
                     /translation="MAKTKGDLVLKALRKAGLYSNATLTDADPQAIEDAINDLEDMMA
                     AWQAKGIELGYQFADTENGIMPLPDDDSGIPAWANDGVALKLAVQVCMDNVIQPSDAL
                     LTAADSAYQTICIALTKIPPLERRNDMPRGSGNKSAFTWNRFYIEKDDPST"
     gene            18173..19591
                     /locus_tag="ACHOQV_002508"
     CDS             18173..19591
                     /locus_tag="ACHOQV_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFP9630869.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYGLQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNI
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            19591..20439
                     /locus_tag="ACHOQV_002509"
     CDS             19591..20439
                     /locus_tag="ACHOQV_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFP9630870.1"
                     /translation="MADSNLNEPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHESRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLMPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNSATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            20439..20918
                     /locus_tag="ACHOQV_002510"
     CDS             20439..20918
                     /locus_tag="ACHOQV_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFP9630871.1"
                     /translation="MEIKLIDNPVKLAEFLNNPANTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYTSNGKPVGITIYQLPRSKYEELKNVNFPDCQ"
     gene            20893..21546
                     /locus_tag="ACHOQV_002511"
     CDS             20893..21546
                     /locus_tag="ACHOQV_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFP9630872.1"
                     /translation="MLIFQIANKHLSKAVYCKGGSDSGAKEQARATEKGIELQREMWQ
                     TNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAAAE
                     ATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYANNV
                     SQLYQQQAAASAANANKPSGFQSALGGAAAGAAAGTAIMPGWGTAIGAGVGLLGSLF"
     gene            21559..23031
                     /locus_tag="ACHOQV_002512"
     CDS             21559..23031
                     /locus_tag="ACHOQV_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFP9630873.1"
                     /translation="MATWQQAGNSGALLAGLGGMNSNAPRASDADATLAYIRQNNEME
                     RSGRNNVGLQALQGISSVMDMYKQMDQQKRQQEFQQAYADAYTSGDRDAMRKLASQYP
                     EQFDAVRNGMKFVDEDQRSTVGTLAASARLAASSPEAMMSWLQNNSSELTRAGVDPLD
                     VAKMYQQNPQGFTEFVDHLGMAALGPEQYFQAQDRIVGREIDRGRLAETIRSNQAGEA
                     LQARGQDISRANALTSAYAPTAAMQNYNQYAQMLKADPDGAAAFAAAAGINPNAKKLL
                     KVETNPDGSVTKYYTDGSEEAGKLNQPISGDGIKPISLPQAQSIIDKANEGSKKAAGF
                     ALRLKDSMDSMNQLSKSIDPKRVALINRSLGDGTIANLSLSPAEQQYMVNARDALYAI
                     LRPETGAAITLPEMQEYSKMYLPQPGDSKAATETKMRKMQGQYNSLRGQSGRVYDALV
                     VSSAANSQQQSNSQQPTNTQQQQSQSGSYTSKSGIQFTVE"
     gene            23031..24869
                     /locus_tag="ACHOQV_002513"
     CDS             23031..24869
                     /locus_tag="ACHOQV_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFP9630874.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     EPAREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTITLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNAAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNALLRLANTKAGTPAYERALNQAATAVRPLLANEATRQ"
     gene            complement(24892..>25224)
                     /locus_tag="ACHOQV_002514"
     CDS             complement(24892..>25224)
                     /locus_tag="ACHOQV_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015975200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein A"
                     /protein_id="HFP9630875.1"
                     /translation="PENEACALNYLKEKFSPEKFENRITDALGLVISVIHMPLSLLIT
                     VWYFAMIAGRIFGFMNIEPVVLWVPMILQLLLSVAIFIFSVFIKIVFGRYPGEAKGFN
                     KEFIKTIK"
BASE COUNT         7234 a         5800 c         6436 g         5754 t
ORIGIN      
        1 gttattgttg tgtattttag agttgtgagg ctgttaacta aatgtctctt caggccactg
       61 gctggcgata actttcccta ctacggaaca gctatcattg catggaatca ttggatattg
      121 cgggtttagt ggctgtagga acacctgacc gctatccctg atcagtttct tgaaggtaaa
      181 ctcgtcacca ccaagtctgg ctatgcagaa atcacctggc tcaacagcct gctcagggtc
      241 aacgagaatt aacatcccgt caggaaagct tggcttggat cctgttggcg cggtcatgga
      301 attaccttca acttcaagcc agaacgcaca atcactggct tttttggttg tgctgaccca
      361 tctctccgca tcacctttgg taaaggttct aagctcaggc gagaacatcc cggcctgaac
      421 atgagaaaaa acagggtact catattgttt tttaacgggg gcagatgagt attcgccaac
      481 aggtgaaaat gtaccgtcgt ggttgaatga gacgttatca ataccaaggt atttaaacac
      541 cacaccaatc tcgtcaagag atggatgacg agatccgcgc aaccagtgac caattccacc
      601 ctgcgtcata ccaagctctt cagctaactt ctcttgagtt atgcctagct ctttcattct
      661 ggatctagcc agttcatacc atttcatttt cataccctta ttattacgct ctgtactaaa
      721 accatccatg cacaagatgt attttttgtt tgcattctaa aagtacatat cgtattattg
      781 tttcatggtt actatggagg gcatatgagc aacctacgaa aatatcgaga gtcactgaat
      841 atctctcaaa caacacttgc taaggcagtt ggatgcacac agggagctat cggacattgg
      901 gaatctggtc gtcgcttccc agaccttaaa acatgccgtg ctcttgttga gtgcctaaac
      961 aagttaggcg caaaagtcag tcttgatgac gtgttcccgc cggaacacaa agccgcttaa
     1021 gacattcccg ctcttacaca tcccagccct gaaaaagggc atcaaattaa accacaccta
     1081 tggtgtatgc atttatttgc atacattcaa tcaattgtta tctaaggaaa tacttacata
     1141 tggttcgtgc aaacaaacgc aacgaggctc tacgaatcga gagtgcgttg cttaacaaaa
     1201 tcgcaatgct tggaactgag aagacagcgg aagctgtggg agttgataag tcgcagatca
     1261 gcaggtggaa gagggattgg attccaaagt tctcaatgct gcttgctgtt cttgaatggg
     1321 gtgtcgttga cgacgacatg gctcgattgg cacgacaagt tgcttcgatt ctcaccaata
     1381 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggaattc tgaggtcatt
     1441 actggatcaa tccacaggag tcattatgac aaaacaactc agtccttacc aggacaaaat
     1501 tcacaaacac atactacgtg atcgcttcct gtccagcttc aagcagcctg gtcgattccg
     1561 ggctgagttg gaaaaagtga agctgatgca gaaggagaaa ggtcatgagt aacatatcta
     1621 atctagccga agccagagag gccagaaggc tacaacaacc gcatcaaagc agcggtaagg
     1681 ggtatgcctt gctgcaccgt aaaattatgg atgtgccgtt ttacaaggac gcagaagctg
     1741 cgcatctgtg ggttcactta atcctcaaag caaagcatac gcctgagtat gtaatgactg
     1801 acgcaggaga aattctggta ggcagaggga agctacttgg cggtagaaac tctctggcgt
     1861 ttgaaacagg actcaaacca gatcgcgttc agtacctgct tagaaagttc aaaaaactcg
     1921 gcatgattga ctgggtttca cacggtaaat tctcagtttt ctcggtagag aaatatgacg
     1981 attatcagtc aaattttgta ccagcagatt accagcaaat taccacagat aaagaatata
     2041 ataatattat ctctaatact gacgtattag agagtaccgc agcagacaaa aagtctgaca
     2101 agaaaaaacc ttccgtcagc tgtcaggatg ttgtcgatgc ttaccacgaa atccttcctg
     2161 aagcgccaag aatccgcgca ctgaatgaca agcgtaaaaa ccagatccga acgttctggc
     2221 gcaaagccgg agtgataacc cgccagcttg acgggcatgg gttcacggtg caggactgga
     2281 gaaattattt gagctacgta ggcgaaaatt gccgatggat gttcgaagag cgcccaaacc
     2341 atcagcgcgg aaccgtctgg cacaaaaagg gatttgattt cctgcttaac gataatacct
     2401 acctgaaagt tcgtgagggt gaacacgatg accgataatt tttatgcgcc gccccatagc
     2461 atcgaggcag agcaggcggt gattggtgga ttgcttctgg atgatgacag cagtgagcgc
     2521 gtccagaaag ttctggcgat gctgaagccc gattcatttt acagccgacc acacaaaatc
     2581 cttttcgaag aaataaccag aatgcaccgg gagcaaaagc cggtagatgg cctgacgctt
     2641 ttcgatgaac tggagcgtaa atcgttaacg gcgtctgttg gcggttttgc ttatatcgct
     2701 gagatcgcaa agaacactcc aagcgcagca aacatcgttg cctatgcaat gcaggttcgt
     2761 gaaaccgcaa tggaacgcta cgccatcaac cgcatgactg aagcgacgga attgctctat
     2821 tcccgcaacg gaatgactgc aatgcagaag tacgaagcta ttcaggcgat tttcacgcaa
     2881 ctgacagacc atgcaaaaac cggatcgcgt cgcggccttc gctcatttgg tgaggtcatg
     2941 gaagactggg ttagcgacct tgagaagcga tttgacccgt caggcgaaca acgaggaatg
     3001 agcacaggga tcccatcgct ggacaggatg ctgtcaccga aaggtctggt gaaaggctct
     3061 ctgtttgtca ttggcgctcg ccctaagatg gggaaaacga cgctatacag ccagatggca
     3121 atcaactgcg cagtgcatga gaaaaagccc gctctgatgt tcagccttga aatgccaggt
     3181 gatcagatac tggaaaaact ggtagggcag aagtctggtg ttaatccgaa tattttttac
     3241 cttccggcga caaatgacgc tgatgacggc tatcagggtg attacgatgg tgacttcaac
     3301 agggcgatag aaacagcaaa tcgcttgagt gaaatcgaca tgctttacat cgacgacacg
     3361 ccgggattat ctctgactca aatcgtcagc gaaagccgtc gaatcaagcg agaaaaagga
     3421 tgtgttggca tgattctggt cgattacctg acactaatga ccgctgagaa ggccgatcgc
     3481 aacgaccttg cttacggcat gatcaccaaa ggactgaaga accttgccaa agagcttgat
     3541 tgcgttgttg tgcttctgac acagcttaac cgcgcactgg aaagccgaac caataaacgc
     3601 ccattaccaa gtgactcacg agatacaggg cagattgaac aggattgcga ttattgggtc
     3661 gggatccatc gtgaaggcgc ttttgatgac agtgttccac ctggtgaaac tgaactaatc
     3721 cttcgtctca atcgtcatgg caataccggc acggtgtatt gcattcaggc aaatggcgct
     3781 atttatgaca cagaccaaca gtccgctgaa atgcgccgcc gtgaacgcga ggaaccgcaa
     3841 tccaagaaga aaggaggatt ctgatgacca tctacatcac tgagctaata acaggggcta
     3901 tttacacagt agcccttttt tattggatcg gaagcgagag gtgagagtgt gagcgagccg
     3961 attatgtctc ctgaccttta tttaaaatat aggtctattt gggggcacag aaaaatgaaa
     4021 gttaatagtt atagattcga cgatgcaact caatgggtaa ctaatttcgg aaaggtaaga
     4081 ataactccgc cgcagtttag aaaactcctt tgcaacaaat gcggaagcga taactggaca
     4141 gatgatggaa gaagcatgaa tgagtactgc tgcgactcat gcggtcagta cgtgatggtt
     4201 cagggcgacg acgcttaaca ccccagcacg ctgatggaga ggaattatga catatccgca
     4261 cattttagaa attcacgaaa aggcggcagc gattaacgca ggacgaaagc ctcagtttgg
     4321 ggacaaaatg cgaaaccttg tggccggtga agacaacccg cgccgcgatg cctacttcgt
     4381 gaaacagaaa agagtgacag gacgaatgaa tcccgggctc tggtacacga tgacagaacg
     4441 caaaggtaac ttctgggact cctgtccgaa aggcctgatt ttcattgacg agtgatggag
     4501 aggaatatgg acgaatcaag aaagcagttt gaagaaagtt ggttgcgacg tggaggcgaa
     4561 tcttcatacc ttatccgtta ccctgaaaat caccatgaaa ttggcagtgg tgatattggt
     4621 ggtcaatacg tgatggacga tgttcaaggc cactggcaaa cgtggcaggc atcgcgagca
     4681 gctattgaaa ttgagttgcc tgaaagcttt accatgcata gtgggcgaac cccatatctg
     4741 tatgtaagcg aggtgcagag cgccatccgc gctgctggag tcaaagtgaa ggagtgagta
     4801 tgagcgcata cgaagaaatc atgttagccc tgcgattctt tttcggtgtg gaagaagatg
     4861 aaaacgtaaa tgagattatc gggcaagacc atgacccgat agggactatt gcagctgcac
     4921 ttgacgatta caggagcgta aatggtgagg aaactaacgt ttgaactaag aagcaccatc
     4981 catcagcaga acgccattca agctatccag caaatccttc cagacccaac caaaccaatc
     5041 gtagtgacca tccaggaacg aaaccgtagc ttagaccaaa accgaaagct ttgggcttgc
     5101 cttggtgacg tctctcgtca ggttgaatgg catggtcgct ggctggatgc agaaagctgg
     5161 aagtgtgtgt ttaccgcagc attaaagcag caggatgttg ttcctaacct tgccgggaat
     5221 ggctttgtgg taataggcca gtcaaccagc aggatgcgtg taggcgaatt tgcggagcta
     5281 ttagagctta tacaggcatt cggtacagag cgtggcgtta agtggtcaga cgaagcgcga
     5341 ctggctctgg agtggaaagc gcgatgggga gacagggcag catgagacga cagcgacgaa
     5401 gtatcaccga catcatctgc gaaaactgca aataccttcc aacgaaacgc tccagaaata
     5461 aacgcaagca aatcccaaaa gaatctgacg taaaaacctt caactacacg gctcacctgt
     5521 gggatatccg gtggcttaga gaacgcgcga ggaaatgaca atgcttttaa ttcaacctag
     5581 atttggcctt agcatcaaaa aagggcacat gtttggtgag aaagagtcac aacgaaaaat
     5641 ggtgtctatc cggttgccat ttatcagtat ttattggcta aacagggagg caacaaatta
     5701 ttggtataca tgcgccagag cagcatttaa cgaccctgac tggtttgtga aaaaccacca
     5761 cgcagttcgt caggcaaaga gaaaggccaa tacgacatac atgaaggcgt atcgaaaagc
     5821 atggaaagaa caccgcgatc gataccagca agacatggaa aagcttgaat cagaaaacat
     5881 ggaattaaga cgaaagcttg gtgaagcaaa acgagacatt gatgcttaca agcgactttt
     5941 taatggtgaa agccatgctt agtccatccc aatctcttca ataccagaaa gaaagcgtcg
     6001 agcgggcttt aacgtgcgct aactgcggtc agaagctgca cgtgctggaa gttcacgtgt
     6061 gcgccgattg ctgcgcagaa ctgatgagcg atccgaatag ctcaatgtac gaggaagaag
     6121 acgatggcta aaccagcgcg aagacgatgt aaaaacaatg aatgtcggga atggtttcac
     6181 cctgcattcg ctaatcagtg gtggtgctct ccagagtgtg gaaccaagat agcactcgaa
     6241 cgacgaagta aagaacgcga aaaagcggaa aaagcagcag agaagaaacg acgacgagag
     6301 gagcagaaac agaaagataa acttaagatt cgaaaactcg ccttaaagcc ccgcagttac
     6361 tggattaaac aagcccaaca agccgtaaac gccttcatca gagaaagaga ccgcgactta
     6421 ccatgtatct cgtgcggaac gctcacgtct gctcagtggg atgccggaca ttaccggaca
     6481 actgctgcgg cacctcaact ccgatttgat gaacgcaata ttcacaagca atgcgtggtg
     6541 tgcaaccagc acaaaagcgg aaatctcgtt ccgtatcgcg tcgaactgat tagccgcatc
     6601 gggcaggaag cagtagacga aatcgaatca aaccataacc gtcatcgctg gactatcgaa
     6661 gagtgcaagg cgatcaaggc agagtaccaa cagaaactca aagacctgcg aaatagcaga
     6721 agtgaggctg catgacgttc tcagtaaaaa ccattccaga catgctcgtt gaagcatacg
     6781 gaaaccagac agaagtagca cgcagactga aatgtagtcg cggtacggtc agaaaatacg
     6841 ttgatgataa agacgggaaa atgcacgcca tcgtcaacga cgttctcatg gttcatcgcg
     6901 gatggagtga aagagatgcg ctattacgaa agaattgatg gcagcaaata ccgaaatatt
     6961 tgggtagttg gcgatctgca cggatgctac acgaacctga tgaaaaaact ggagacgata
     7021 ggattcgaca ccaaaaaaga cctgcttatc tcggttggcg atttggccga tcgcggtaca
     7081 gagaacgttg aatgcctgga attaatcaca ttcccctggt tcagagctgt acgtggaaac
     7141 catgagcaaa tgatgattga tggcttatca gaacgcggaa acgtcaatca ctggctgctt
     7201 aatggcggtg gctggttctt taatctcgat tacgacaaag aaattctggc taaagctctt
     7261 gcccataaag cagatgaact tccgttaatc atcgaactgg tgagtaaagg taaaaaatat
     7321 gtcatctgcc acgccgatta tccttgtgac gaatacgagt ttggaaagcc agttgatcat
     7381 cagcaggtaa tctggaaccg cgaacgaatc agcaactcac aagacggaat cgtgaaagaa
     7441 atcaaaggag cggacacgtt catctttggt catacgccag cagtgaaacc actcaagttt
     7501 tccaaccaga tgtatatcga taccggcgca gtgttctgcg gaaatctcac attgattcag
     7561 gtacagggag aaggcgcatg ggaataagag aactaaacct caccaaagag cagcacgagt
     7621 ggctgaatgg ctggcttgaa ctgtggggcg catgggttta ttcaggtcgt ctggaaaagc
     7681 gcatgagcag cgtaatagcg aagttcatgg agagcgtaga gccgggaaga gttatgacaa
     7741 ggccaatgtg caatgatgat gatggaatgt tgatttctca ggtcgtcgat tccgtcatgt
     7801 gcattgacaa gaaagccttt ggcatcctcc tcagctacta cgctcatggt tcatctaagc
     7861 gagcaattgc atcctactat cacgcgactg caaagccacg caagatgtgt ggacgtggtg
     7921 gcgagggatg gagaaaacct tcactggcaa cctgtagaaa cgaaattgac gacatcctga
     7981 aagcgtcgtt atttgttttg taccagccaa tgcaaaatgc tttcaaaatg cgtaaacgtg
     8041 ttgagaaagt taagcatgtt gctgttaaaa gccttgacat gcaattatcc atttagccat
     8101 aattagaagg taagctgccg ttagtgactc ttaagttgca acggtggctt tttttatttg
     8161 ggtaagtcgt ataaaggtca ttacggaagg ctgttaacct tcttatcgtg gttcgagccc
     8221 atgctgtccc gccaaatatg ctggtttagc tccaatggta gagcagtcgc cttgtaagcg
     8281 aatgggtagc ggttcaagtc cgttaaccag caccataact gagccgtagc cactggctat
     8341 cctgaattca tcagtgatag ttatgctgcg gccttctaca catgaccttc gtgaaagcgg
     8401 gtggcaagaa gttgcgctaa caacctcctg ccgttttgcc cgtgcatatc ggtcacgaac
     8461 aaatctgatt actaaacaca gtagcctgga tttgttctat cagtaatcga ccttattcct
     8521 aattaaatag agcaaatccc cttattgggg gtaagacatg aagatgccag aaaaacatga
     8581 cctgttagcc gccattctcg cggcaaagga acaaggcatc ggggcaatcc ttgcgtttgc
     8641 aatggcgtac cttcgcggca gatataatgg cggtgcgttt acaaaaacag taatcgacgc
     8701 aacgatgtgc gccattatcg cctggttcat tcgtgacctt ctcgacttcg ccggactaag
     8761 tagcaatctc gcttatataa cgagcgtgtt catcggctac atcggtactg actcgattgg
     8821 ttcgcttatc aaacgcttcg ctgctaaaaa agccggagta gaagatggtg gaaatcaata
     8881 atcaacgtaa ggcgttcctc gatatgctgg cgtggtcaga gggaactgat aacggacgtc
     8941 agaaaaccag aaatcatggt tatgacgtca ttgttggcgg agagctattt accgattact
     9001 ccgatcaccc tcgcaaactt gtcacgctaa acccaaaact caaatcaaca gcagcaggac
     9061 gttaccagct tctttcccgt tggtgggatg cttaccgtaa gcagcttggc ctgaaagact
     9121 tctctccgaa aagccaggac gctgtggcat tgcagcagat taaagagcgt ggcgctttgc
     9181 cgatgattga tcgcggtgat atccgtcagg ctatcgaccg ttgcagcaat atctgggctt
     9241 cactgccggg cgctggttat ggtcagttcg agcataaggc tgacaacctg attgcaaaat
     9301 tcaaagagtc tggcggaacg gtcagagaga ttgaggtatg agcagagtca ccgcgattat
     9361 ctccgctctg gttatctgca tcatcgtctg cctgtcatgg gctgttaatc attaccgtga
     9421 taatgcaatc gcctacaaag aacagcgcga taaaaaagtc agtgagctga agcaggcgac
     9481 cgccaccatt actgacatgc agcagcgcca gcgtgatgct gatgcacttg atgctaaata
     9541 cacgaaggag ttagctgatg cgaaagctga aaatgatgct cttcggcgca agcttgataa
     9601 tggtggccgg gtgctcgtca aaggaaaatg ctctgtgcca tcctcagccg aaacctccgg
     9661 cgcctccggc atgggcaatg attccaccgt cgaactctct ccagttgctg gacgaaacgt
     9721 tctcggtgtc cgagacggaa ttatccgcga ccaaacagca ctgagaacgc ttcaggaata
     9781 tatcaggacg caatgccttc gatgatagcg ataattttac tcatcatcct tcacatctgg
     9841 ctctgtagac agggtggtgg tcacttctgg agtaaatcca gattaaatat ctcattgttg
     9901 atgcttgata ttgagcatct ggcgcgcggt aaggggctgc gttgagataa gagcctgtca
     9961 tcacaaacac caggatttag cctcgcattc gcggggcttt tttatatctg caacaaacgc
    10021 gcttcacacg cgcgacttct gaacacagaa cctttcagga tgacccttga ggatgccggt
    10081 ttggtgatcg gtgcctttct gtgggccgga atcctgtgtg acaaggttca tcactaaaag
    10141 gtaaccactg atgaagtacc caacagttat tgtcaatggt gtgtccgttc gtgttgatga
    10201 ggatggacgc tacaacttaa acgatctcca tgcagcagca gttgcaaatg gagaggctac
    10261 agagcaacag cgcccaagcc agtttttgcg tagcgcgcag ataaaacgct tcataaaagc
    10321 actggaggcc aaagtgcaaa aaagcacttt ggaacaaatt caaccactta aaataatcaa
    10381 aggtggtgcg gaaccaggtg tgtggggtgt tgaacttctg gcaatcagat atgcagcatg
    10441 gattaagccg gaatttgaaa tcgaagttta tgaagttttc aaaacggttg tccgtctcgg
    10501 cgttggcgca atgtcccgtc tgaatagaat cgatcacatc atcaatactg aaaccaaagc
    10561 gataagccag tgcgcaagcc aaatggctaa gtggggcgtt ggtgggcgaa aaagattgct
    10621 tcatgttgca cgtgagagag cggcaaatga agtgcaaatg tatttgcccg gaatggtgtg
    10681 atttcgcagg ttaatccagt ttgtacatta cggcagtacc acgaagcaac ccaagccaga
    10741 aagtggggaa ataacactgg cagccactga aagatgaacc tccagcctta tggcaaaaaa
    10801 gattctttgt ggtggcggac tgatggaaag acatcggtta ttgcagagac cattcaatga
    10861 gtggtctcga caatggctta taccttacac gggataactt aactgatatc ccttttaacg
    10921 gataaacgga gccaacaatg gcagagatta cccccatgac tgaagaacag aaattccaga
    10981 tagagattta caaactggtc atgaaccaga acgcagccgc agaggaagca tttcagttca
    11041 ttggcactga cgaactgaag cttgagctat tcaaaattca cttccagtca ggcggcgcta
    11101 attcagatac cacgacccgc actatcgaag cggtgcgtaa atcgaaggaa gcgttagacc
    11161 tgttcactac cggagcataa acatggcgcg cccaacaaag tatcaagagg cgtacgccga
    11221 acaggcacgc aaactgtgct tgctgggcta caccgatgca gagcttgctg atttcttcga
    11281 agtcagtgag tcaactatta acaagtggaa gcttgattat cctgagtttt cggagtccat
    11341 aaaaaagggt aaggccgtcg ctgatgcaga agttagtgac cgtctttatc aacgcgctat
    11401 gggcttcgtg gccccagaca ttgatattcg tgttattgaa aacagaattg tcgaaactcc
    11461 gcttgagaag tattacccgc cggatacaac agctgccatt ttctggctta agaaccgaca
    11521 gaaggataaa tggcgcgaca aggttgatca cgagctaaca ggcaaagacg gcggcgcaat
    11581 ccagattgaa acatcaccga tgagcactct atttgggaaa tgacctcgat taatcctatc
    11641 tttgaaccgt tcattgaggc gcatcgctat aaagtcgcca aaggcggtcg aggtagcggt
    11701 aagtcatggg caattgcgag actgcttgtt gaagcggcgc gtcggcagcc ggtgcgtatt
    11761 ctctgcgctc gtgaactgca aaacagtatc agcgattcgg taatccggtt gcttgaagac
    11821 accatagagc gggaagggta ttcggctgag tttgaaattc agcgttcaat gattcgtcat
    11881 ctcggaacga atgctgaatt catgttctac ggcataaaaa acaaccctac gaagattaaa
    11941 tcgctcgaag gcattgatat ctgctgggtg gaagaagcgg aagcggtaac aaaggaatcg
    12001 tgggatattc tgatcccaac catccgtaag ccgttctctg aaatatgggt gagcttcaac
    12061 ccgaaaaaca tcctcgacga tacctatcag cgattcgtcg taaatcctcc cgatgatatt
    12121 tgcctgctga cggtcaacta caccgacaat ccgcactttc ctgaagttct ccgtctggag
    12181 atggaagagt gtaaacgcag aaacccgaca ctgtatcgtc acatctggct tggtgagcca
    12241 gtaagcgcaa gtgatatggc aatcatcaaa tgtgaatggc ttgaagccga aaccgatgcg
    12301 cacaagaaac tcggatggaa agcgaaaggc gcggttgttt ctgcacatga cccatcagat
    12361 acagggccag atgctaaagg ttatgcatcg cgtcacggtt cggtagttaa gcgcattgcc
    12421 gaaggtctgc tgatggacat caacgagggt gctgactggg ctacttcgct ggcgattgaa
    12481 gacggcgctg accactacct gtgggatggt gatggtgttg gtgccgggct acgcagacag
    12541 acaacggaag cgttctccgg caagaaaata accgccacga tgttcaaggg cagcgaatcg
    12601 ccattcgatg aagatgcgcc ttatcaggcc ggggcatggg ctgatgaagt cgtacagggt
    12661 gacaacgttc gcactattgg cgatgtgttc cgcaataagc gagcgcaatt ctattacgca
    12721 ctggctgaca ggctgtatct gacatatcgg gcggttgtcc atggggagta tgcagatcct
    12781 gacgacatgc tgagtttcga caaagaagcg ataggcgaga agatgctgga gaagctgttt
    12841 gcagaactga cgcagattca gcgcaaattc aataacaacg ggaagctgga gcttatgact
    12901 aaggtcgaaa tgaagcagaa gctcggtatt ccatctccta acctggctga tgcgctgatg
    12961 atgtgtatgc attgcccgga gtcggctgcg caacccgact attccagtta ctcaattcct
    13021 tgtggtgtag gttgatatgg cagaaaaaaa gatgactgac tggcatcgca aggtgctgtg
    13081 caactttgat aatgcctggt cagcaacgca ggatatgcgt gagcagatta ttgaggctca
    13141 gcgtttcgtc cgggtgtccg gcgcacagtg ggaaggcagc acaaacgctg gttactcatt
    13201 tgatgaaggc aggtttgagc attatccgcg ttttgaactg aataagattg cccgtgaatg
    13261 tgatcgcatc attggcgagt atcgacagaa tcgcatcagc gttaaattca ggccgaagga
    13321 tgacaaggca tcggaagcgt tagccgaaaa aatgaacggc aaattccgcg ctgactatca
    13381 ggaaacatcc ggtggcgaag cgtgtgataa cgcatttgat gatgctgtaa cgggcggatt
    13441 cggttgtttc cgcatgtgtg ccgattacga agatgaaatg gatccgagta acgagcagcg
    13501 ccgcatcagc cttcttcctg tttacgaccc agcgacatgc gtattcttcg atcaggacag
    13561 caagcaatat gaccgctctg atgctatgtg ggctatggaa atgttctcca tgacgcctaa
    13621 agcgttcgag gctgaatacc ctgattccat cgcggcaagt ctttctcgtg atgacactgg
    13681 tactcagtat gactggtcaa cgcctgacgc tatctatgtt ggacgttact acgaagtccg
    13741 catagagaag gtgaagctcg cagcatggcg taaccctgtc agcggagaaa cggcaatcta
    13801 tgatgaagag caaatcaaag atattgtcga cgagctgacc gatggtgcat tcgaactgat
    13861 tggcgagcga acggtgaaga aacgccgagt ttattgcggt cttctgtctg gcgctgaatg
    13921 gctggaagaa cctaagcgta ttccgggcga acatattcct ctcatcccgg tatatgggcg
    13981 tcgttcattt gttgataatc aggagcgaat cgaaggccac gcagcaaaag cgatggatgc
    14041 acagcgtctt gagaacctga tggtttccat gattgcagat aacgctactc aggctggcgg
    14101 tgatggcatt cctatcgtgg atgttgattt cattcccggt ccattaatga atcactgggc
    14161 agagaggaat aagaaaagac ctgcagttct tcccatgacc agcaagaagg acaaaaacgg
    14221 aacggtcatt tcagaggctc aggttgctgg ctggacacct ccgacacaaa tgcctcctgc
    14281 tcttgccggg ctattgcagt acaccgggac ggctattcag caaattacag gtgcgtcgca
    14341 gcttgagaac atgccgagca acgtcgctac cgataccgtt gatagcatct ttaaccggat
    14401 ggacacgcag tcctatatct acatggacaa catggctaaa tccatgcgcc gtgctggcgt
    14461 cgtgtggctt tctatggctc gtgaagtcta tggcagcgat acgccaatgc gcatcgttaa
    14521 tgaggatggc agcgatgacg tggcgctgat gactggtgaa gtggttgacc gtcagacagg
    14581 gcaggttatc gcgcttaacg acctttcgca gggtaactat gaagtgactg tcgatgtcgg
    14641 ccagtcgttc gctactcgcc gtgatgcaac ggttaagtcg ttactttcca tgctggcact
    14701 tatcccacca ggaacgccga agcacgacct tgtatcgtcg atgattctcg acaatatgga
    14761 cggcgaaggg atggacgacc ttaaagaata caaccgcaat cagttgcttc tgtctggagt
    14821 tatcaagccg agaacgccag aagaacagca gatggttgag caggcgaaac aacaacaggc
    14881 cagtcagcca gatcctgcta tggttgctgc gcaaggtcag cttcttgctg gtcaggctga
    14941 attgcagaaa gcgcagaacg aacaggcagc cattcaggtt aaagcattcc aggcacagac
    15001 tgatgctcag gttgcagcgg caaatgttgt gaaaatcctc gcatctgccg atagccagca
    15061 gaaatctgat atccgcgagg ctctgaaact gctcggacag ttccagcaac agcaaggaga
    15121 caatgcccgt gctgatgcag agcttgtcct gaaaagtcag gcacagggcc atgcgcagcg
    15181 catggacatc agcagcatcc tgcaaaaatc aactcagcaa caaccacagc agtaattaac
    15241 ccataacgtg caatggctgt ctttatgagg cctggcaccc tattgccttc cgatgggctg
    15301 aacatcgagt aaacaggggt aacaaatgga ccagatggca gaaaacacac cagaagttga
    15361 aatcgaaacc gacgcgtcag agcagattcc tgatgatgtc gaactggctg aagaagtcga
    15421 aacagaagat ggcggtgagt cctctggcaa tgatgcagag gaagctactg aaactgatga
    15481 cgacgaatca gaacaggaat tctactttgg tgacgaaaag ctggattcgc caaccagcga
    15541 agatagcgca gagcatggac tggtaaaaca cctgcgcaag acgattaaag agaaagaccg
    15601 cgagctgaaa gagctgatgc gtcagtctca gaaacccgtc gagcagcagc cggtaatcac
    15661 tcaaccaccg cgaatgccaa aactggatga tgaggacatc ggtttcgatg aagaaatcta
    15721 tcagcaacgc atggctaagt gggcagagga taacggaaag taccagcaac aggagatggc
    15781 tcgcaagcag aaggagcagg agcttcaggc tgcttatcaa gagcgattat ccaaatatca
    15841 gcaacgtgtt aaggctctca aagttcctgg ctatcaggaa gctgagcagg ccgtactcga
    15901 ggaaatcccc atcgagacac aaaacgcgat cttgtttgag tcagagaagc cggaaatcgt
    15961 tgttctggca ctcggtcgca acgctgaact gcgcaagcaa ctggcagaag ctaccaaccc
    16021 cgtagcaatt ggtcgtctgc tggaacgtat cgaatcgaag gccagaatca tgccaaaagc
    16081 aaaaaccacg gcagccacaa ccccgacagt taaggggagc aacggcgcag taatcaacaa
    16141 cctcgacaaa ctgaaagcca aggcgctgga aactggtgac tggacgccgt atttcgccgc
    16201 taaaaaggca aaaaaataac ctatcggagc attaagcatg gctaaccaat tagcaaaaga
    16261 ccttgaaatc atgttcgaaa actacgttga aggctttgag gccgcctgcg tagtttcccg
    16321 taacgctaaa aaattccgtc ccggtgatac agcaatgcag cgagcaggtg atgttctgta
    16381 tcgtccgcag cattaccaca tgaacattga ggaaggccta gacctcagcg gcaaaacgcc
    16441 aacagcactg gttcagcgcc ttgttccttc tgtgttcaag gagccgaaaa acattctgta
    16501 cactctggat gcgcgtgaaa tgcgtgaccc ggaacataaa actgaagctg gtcgcgccgc
    16561 aggtatgcgc cttgctgcac agattgactc tgacctgatt tccatggtta cgcagcgtgc
    16621 tactaacgtg atcacgatgg ctgactcaac cacagggtca cagggccgtg atttgtggaa
    16681 ctgtgcggca ggtattgatg ccaccatgac ggcgattggt gtacctcagg gtatcaaccg
    16741 tcgctctttc tggaacccct tcaactacaa agaccttgct ggcgagcttg gtcaccgtgc
    16801 ctatgctcag ggcgcaaccc tgacagcata cgaaaaagcg caaatccctc cggttgcgtc
    16861 cttcgatagc tacaagaccg atatttctgg tcgtgttccg aagggtacag caacttccat
    16921 tacgctggca gcagcacctg cgcacaaggt tgaagcgaaa gatgctaacg atatgccagt
    16981 ggataaccgg caggggacca ttacggtatc tgctgaaggt ttgcaggttg gcgatgcgtt
    17041 taccatcgca ggggtgaatt ctgtacacca gatcaccaaa gataccaccg ggcagccgca
    17101 ggtattccgc gttctggcag taagcggaac gacagtaact atctccccga aaattctgcc
    17161 gcctgacaac gcggatgtcg ccagccgacc atatgcaaac gttgatgcta atgcggcaag
    17221 tagcgcagca atcaccattc tcaacaaaaa tgccgcaccg gctaacctgt tctgggctga
    17281 tggttctgtt gaactgatgt acggcaagct ggcgttccca actggtcagg gtccacaagt
    17341 aatgacagca accaccgagc agggcgctac gctgatcatg tcttacgcct tcgaccacat
    17401 caaaggtgta accactgcgc gtttcaccac cctgtacggt tgctctgtac ttgttcctga
    17461 atatacgggc atcgttattg ccgggcagta attttggtgg ggcttcggcc ccatttttat
    17521 tgggagaaga caatggcacg aacaatgctc tataagccgg gcaacatgat cacctgtggt
    17581 cagtttgctg tcgattacat cattgttgat gacgaagaag ttaaatctca cctgaaaaaa
    17641 ggctgggtaa aaactcctga agaaaccgca acgaagcaaa aagtggctaa ggcggaagaa
    17701 gatggcgaaa acgaagggtg atctcgttct taaggcttta cgaaaagccg ggctgtattc
    17761 caatgccacg ttgacagatg ccgaccctca ggcaattgaa gatgccatta atgacctcga
    17821 agacatgatg gcagcatggc aggcgaaagg tatcgagctt ggatatcagt ttgcggatac
    17881 agaaaacggc atcatgccgt tacctgacga tgattcaggt atccctgcat gggcaaatga
    17941 tggcgtcgct ttgaagctcg ctgtgcaagt gtgcatggat aacgtcattc agccgtcaga
    18001 cgctctcctt accgctgctg acagtgcata tcagacaatc tgcatcgctt taaccaaaat
    18061 accaccactt gagcggcgaa atgacatgcc tcgcggtagt ggtaacaaaa gcgcgtttac
    18121 gtggaatcgg ttttacatcg agaaagatga tccgagtacg tgaggtgaat aaatgccgat
    18181 tcagcaactt ccgctcatga aaggtgtcgg caaagacttc cgaaatgccg actatatcga
    18241 ctatctgcca gtgaatatgc tggctacacc caaagaaatc ctgaacagca gcggatatct
    18301 tcgctcattc ccgggcattg ccaaacgctc tgatgtgaac ggtgtatcgc gcggcgtcga
    18361 gtacaacatg gcgcagaatg ctgtttatcg tgtgtgtggt gggaagctct acaaaggaga
    18421 aagtgaagtc ggtgatgtcg ccggaagtgg tcgcgtatca atggcgcatg gtcgaacatc
    18481 acaggctgta ggcgttaatg gtcaactggt cgagtatcgt tatgatggca cggttaaaac
    18541 cgtctcaaac tggcctacag acagtggatt cacgcagtat gagttaggtt cagttcgcga
    18601 cattactcgc ttacgtgggc gttatgcgtg gtcaaaagat ggtactgatt catggttcat
    18661 cactgacctt gaagacgaat cacatcctga ccgttacagc gcacaatatc gtgctgagtc
    18721 tcagcctgac ggcatcatcg gaatcggaac atggcgagac ttcatcgtct gctttggttc
    18781 atcgacgatt gaatatttct ccctgactgg cgcaaccacc gttggtgctg ctttgtatgt
    18841 cgcacagcca tcactgatgg tgcaaaaagg catcgccggg acttactgca aaacaccatt
    18901 tgctgattct tatgcgttca tcagcaaccc tgcaacaggt gcgccgtctg tgtatatcat
    18961 cggctctggt caggtatcac caatcgccag cgcgagcatt gagaaaatac tacgctccta
    19021 cactgctgat gaactggctg atggcgtgat ggaatcgttg cggtttgatg ctcatgagtt
    19081 gctgattatt cacctgccgc gccatgttct tgtttacgac gcatcttcaa gcgccaatgg
    19141 tccgcaatgg tgtgtgctga aaacaggctt gtatgacgat gtgtaccgcg ctatcgactt
    19201 catttacgaa ggaaatcaga taacgtgcgg cgataagctg gaatcggtga ccgggaaatt
    19261 gcagttcgat atcagcagtc agtatgggct tcaacaggaa cacctgctgt ttactccact
    19321 gttcaaagcg gataacgcca gatgcttcga tctggaggtg gaatcatcca ctggcgtagc
    19381 tcagtacgcc gaccgcctgt tcctctctgc aaccactgac ggcataaatt atgggcgtga
    19441 gcagatgatt gagcagaatg aaccgttcgt ttacgacaaa cgcgttttgt ggaaacgagt
    19501 agggcgcatc aggaaaaaca ttggtttcaa attgcgcgtt atcactaagt cacctgtcac
    19561 tctgtctggc gctcagataa ggattgagta atggctgatt cgaatctcaa tgagccagta
    19621 atcatccagg ctacgcggct cgacacatca gttcttccac gcaatatctt ctcgcagtcg
    19681 tatctgcttt atgttatcgc acagggcact gatgttggta acgtggctaa caaggccaac
    19741 gaagccggac agggcgctta tgacgcacaa gtcaggaacg atgagcagga tgtgattctc
    19801 gctgaccatg agcagcgaat ttctgctgcg gaagcaacgc ttgttaatca tgaggagaga
    19861 atcagccagg cagaatcaac tcttcaggaa catgaaacgc gaatcgctca gaatgaaagc
    19921 gatattgcgt cgcttgatac cagagttcag tcgctggagt cgcaggtttc agaccatgaa
    19981 tcgcgcatcg atgctctgga gtatgccact actcgcaaga agtcagaggt tgtttactct
    20041 ggcgtatctg taaccatccc gacagcgccg accaaccttg ttagcctact gaaaacgctc
    20101 atgccgtcat ccggcacgtt ggcaccattc ttcgacaccg ttaacaacaa gatggttgtg
    20161 ttcaacgaga acaaaacctt gttcttcaag ctgtcgatcg tcgggacgtg gcccagcgga
    20221 accgccaaca ggtcaatgca gctaaccttt tccggctctg ttcctgacac actggtaagc
    20281 agtcgcaact cggcgacaac aaccgataac attctgttag ctacgttctt cagcgtggat
    20341 aaagacggct ttcttgccac aaatggcagt acgttaacca ttcagtcaaa tggtgcggcg
    20401 tttactgcca caaccatcaa aatcattgcg gagcagtgat ggaaataaag ctcatcgata
    20461 atccggtgaa gcttgcggaa ttcctcaaca acccggcaaa cacgggaaat atcgtagaca
    20521 gtggagataa atactacatc aagcctgatg cggtatatct cggcatctac gaaggattag
    20581 tgctggctgg agttcatgaa gtgcgtaact tctggcatag cgttgttgaa tgccatgcgg
    20641 tgtatgaccc cggattccgt ggagaatatg cactgcaagg gcatcgatta ttctgcaaat
    20701 ggcttctcga aaactcacca ttccttaaca gcatcaccat ggttcctgac accaccaaat
    20761 acggacgggc aattatccgt ttgcttggcg ctacccgtgt tggtcacctt gatgatgctt
    20821 ataccagcaa tggaaagcct gtaggcatca cgatttatca gttaccgcgc tcaaaatacg
    20881 aggagctaaa gaatgttaat tttccagatt gccaataagc acctcagcaa agctgtgtac
    20941 tgcaaaggcg gcagtgatag cggagcaaaa gagcaggccc gcgcaactga aaagggcatc
    21001 gaactgcagc gtgaaatgtg gcagacgaac atgcaaaacc ttgcaccgtt cacgccactc
    21061 gctcagcagt acgtatcaca gctgcagaat ctttcctctc ttcaggggca aggtcaggcg
    21121 cttaaccagt attacaactc tcagcagtat aaagaccttg cagggcaggc gcgttaccag
    21181 agtctggcag cagcagaggc aacgggtgga ttaggctcta cagcaacagg aaaccagtta
    21241 gctgcaatcg cacctacact cggtcaaaac tggctgtcag gtcagatgaa caactacaac
    21301 aatctggcaa atatcggcct tggtgctctt acaggtcagg caaacgccgg acagaactac
    21361 gctaacaacg tcagccaact gtatcaacag caggcggcag catctgcggc taatgcgaat
    21421 aaaccatcag gatttcagag cgccttgggt ggagcggcag caggtgcagc tgcaggtact
    21481 gcaatcatgc ctggttgggg tacagcaatt ggtgctggcg tcggtcttct tggttcactt
    21541 ttttaatgga ggtgtctctt ggctacatgg caacaggctg gtaattcagg cgcgcttctt
    21601 gccgggttag gcggcatgaa ctccaacgct ccaagagcaa gtgatgcaga cgctacgctt
    21661 gcatacattc gacaaaataa cgagatggag cgttcaggac gtaataacgt tggcttgcag
    21721 gctttgcagg gcatttcatc tgtcatggat atgtataagc agatggatca gcagaagcgg
    21781 cagcaagagt ttcagcaggc ttatgctgat gcatatacat ctggtgaccg cgatgcaatg
    21841 cgaaaactgg catcacagta tcctgagcag tttgacgctg taagaaacgg catgaaattt
    21901 gtcgatgaag accagcgttc cactgtcggc acactggcag caagtgccag actcgcagct
    21961 tcatccccag aagccatgat gtcatggttg cagaacaact catctgagct tactcgtgcc
    22021 ggagtagacc ctctggatgt ggctaaaatg tatcagcaaa atccacaagg tttcacagag
    22081 tttgttgatc accttggaat ggctgcactt ggtccagaac aatattttca ggcacaagac
    22141 agaatcgttg gtcgtgagat tgaccgaggc agactggcag agacaatccg cagcaatcag
    22201 gcaggagagg cacttcaggc gagagggcag gatattagcc gagcaaatgc gttaacgtca
    22261 gcatatgcac caacagccgc aatgcagaat tacaatcagt acgcgcaaat gttaaaggcg
    22321 gatccagatg gtgcagcggc atttgcggca gcggcgggaa ttaatcccaa tgctaagaaa
    22381 ttacttaagg ttgaaaccaa tcctgatggc tcggtaacta agtattacac cgatggcagc
    22441 gaggaagcag gaaaactaaa ccaacctata tctggtgatg gcattaaacc aattagcttg
    22501 ccacaagcgc aaagcatcat agataaggct aatgagggtt ccaagaaggc ggcaggattt
    22561 gctttgcgat taaaagattc aatggactca atgaatcagc ttagtaaaag cattgaccct
    22621 aagcgagttg cattaataaa tcgctctctt ggtgatggga ctattgcaaa tttaagccta
    22681 tcaccagcgg agcagcaata tatggtaaat gcgagagacg ccttgtatgc aattttgcgc
    22741 ccagaaacag gtgcagcaat tactctgcca gagatgcagg agtattccaa aatgtacctg
    22801 cctcagcccg gtgattccaa ggctgctact gaaacaaaaa tgcgaaaaat gcagggccaa
    22861 tataactcat tacgtggtca gtctggtcgc gtttatgatg ctttggtggt ttcaagtgct
    22921 gcaaatagtc aacaacagag caatagccaa caaccgacaa atacccaaca gcagcagagt
    22981 caatccggat catatacctc aaaatcaggc attcaattta cggtggaatg atgaaagtaa
    23041 ctgcaaacgg taagacattt accttccctg atggtacgag cacggaagat attggcaccg
    23101 ccattgatga gtattttgct ggtcaggctg ttcagcaaca aacagttaat caggccaata
    23161 atgaaccagc acgtgaagaa ccatcattga tgcaacaagc tggcgattgg ctcactggtg
    23221 gtcaaagtgc agggcaaatt gcagaacagg ctggtcgtgg tctggtaaac ataccatttg
    23281 acgtattgca gggtggcgca agtctgatta atgcaatcag tcaggggctt ggtggcccca
    23341 aggttttgga tgatgtttat cgtccagtag acagaccgac agacccctac gctcaagctg
    23401 gagaaacaat tggtgggtat ttagttccag gagttggaac ggcaggaagc atggctattg
    23461 gatcactggc agaggccgca aatcagaaag gcgatttcgc acaaaacgca gctaaaaatg
    23521 ccggagttaa ccttgccgct cagggtgttc tttccgcagc agcaaaggga atagggcgtg
    23581 gaataacggc tataaaaggt gatattgcgc cagaagtggc gaagaaaatt gccacatcag
    23641 aatcgatggg cgtgacacca atgacatctg atgttatccc gccgaaaaat gctttcactc
    23701 gcggccttac tcaggatgcc gagggggctt tgctcgggac aggctcaaag cgagcggagc
    23761 aatatgcaac gcgtagtaag ttggtaagta attattttga ccgttttggt gagtacaacc
    23821 cggatgatgt ggtgaaatct ctgaccatca cgttaagggg gcggaaggat gctgctggcg
    23881 ctgttatcaa tgacgtcacc aataaaatgg gtaatgccgc agttgatacc acaaatacca
    23941 tgaatgctct gaatgcagcg atcgcaagac aggaacgact tgggacttca gccaatcaaa
    24001 gcctgcttac atccttgcgt aacctacgtg aagaattagc taaccctgca actgatttgg
    24061 atgttacgtt tgatctcttg cgtcagcaca gaacagcatt tagatctaat gttcagggag
    24121 atgctatggt cttccccaac caggcaaaag cagctaccaa tatggtagag aatgcaatgt
    24181 caaaagacct tcgtaacgca gttgcaaaaa accttggtgc gtcagacgca gcaaaatacc
    24241 ttaaagcaaa ttccgattat gcaaacgttt ataataaggt gcttaataaa aacattgcta
    24301 acaaactcaa caaggcaagc agtgaagcca gtcctgaact tataaatacc gttgtattaa
    24361 gcagaaaacc atctgacgtg aaacgaatct ggagcgcact ggatgataaa gggaaagatg
    24421 ctatgcgtgc agcttacgtc agcaaaatag cggaaaaggc cggtgactct ccagccaagt
    24481 tcatcactga agttaataag ctgaaatctc agtcaggcgg tgaaatttac aacactattt
    24541 tttctggaaa gcacatgaaa gagcttgatg ctcttcatga agttctacag caaacagcaa
    24601 ggtcagacac cgcaaatgta gtaactcaga cggggcaatc acaagccaac aggataagga
    24661 cgattggcgc aactgcgact cttggagtat caatggggct tgaggctggt tttggtgcaa
    24721 tgatgcgttt gtatgagtcc aaagcagcaa ggaatgcgct cttacgtctg gcaaacacta
    24781 aagctggaac gccagcttat gaaagagcgc taaatcaggc tgctactgcc gtgcgcccgc
    24841 tcttagctaa cgaagcgacc cggcagtagc actgtaagcc aaggacggca tttattttat
    24901 agtttttatg aattctttat taaatccctt agcttctccg gggtatcttc caaagacaat
    24961 ttttataaaa acagaaaaaa taaagatagc aacgcttaac aacagttgca gtatcattgg
    25021 aacccaaaga actacaggct ctatattcat gaaaccaaat attcttccgg cgatcatggc
    25081 gaagtaccac actgttatca gcaaacttag tggcatatga attactgata ttaccaatcc
    25141 aagagcatca gtaattctat tttcaaattt ttcaggggaa aacttttctt taaggtaatt
    25201 cagtgcgcag gcctcattct ctgg
//
LOCUS       DAZKGF010000067        24653 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZKGF010000067 DAZKGF010000000
VERSION     DAZKGF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.067"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            198..968
                     /gene="yjjP"
                     /locus_tag="ACHOQV_002515"
     CDS             198..968
                     /gene="yjjP"
                     /locus_tag="ACHOQV_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFP9630876.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            959..1432
                     /gene="yjjB"
                     /locus_tag="ACHOQV_002516"
     CDS             959..1432
                     /gene="yjjB"
                     /locus_tag="ACHOQV_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFP9630877.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1540..2079
                     /gene="dnaT"
                     /locus_tag="ACHOQV_002517"
     CDS             1540..2079
                     /gene="dnaT"
                     /locus_tag="ACHOQV_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFP9630878.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2082..2819
                     /gene="dnaC"
                     /locus_tag="ACHOQV_002518"
     CDS             2082..2819
                     /gene="dnaC"
                     /locus_tag="ACHOQV_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFP9630879.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2868..3362
                     /gene="yjjA"
                     /locus_tag="ACHOQV_002519"
     CDS             2868..3362
                     /gene="yjjA"
                     /locus_tag="ACHOQV_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFP9630880.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3616..5907
                     /gene="opgB"
                     /locus_tag="ACHOQV_002520"
     CDS             3616..5907
                     /gene="opgB"
                     /locus_tag="ACHOQV_002520"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFP9630881.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6045..7067)
                     /gene="lgoD"
                     /locus_tag="ACHOQV_002521"
     CDS             complement(6045..7067)
                     /gene="lgoD"
                     /locus_tag="ACHOQV_002521"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFP9630882.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGALVAVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7206..8120
                     /gene="lgoR"
                     /locus_tag="ACHOQV_002522"
     CDS             7206..8120
                     /gene="lgoR"
                     /locus_tag="ACHOQV_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFP9630883.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8335..9696
                     /gene="lgoT"
                     /locus_tag="ACHOQV_002523"
     CDS             8335..9696
                     /gene="lgoT"
                     /locus_tag="ACHOQV_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9630884.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMIGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9745..11409)
                     /gene="tsr"
                     /locus_tag="ACHOQV_002524"
     CDS             complement(9745..11409)
                     /gene="tsr"
                     /locus_tag="ACHOQV_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFP9630885.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAASEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11591..12454
                     /locus_tag="ACHOQV_002525"
     CDS             11591..12454
                     /locus_tag="ACHOQV_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9630886.1"
                     /translation="MAYSIDIISCITERFMALTATEKRIAQFILDDVSAAVELPIAEL
                     ARLTQTSQASVTRFARALGCKDVRELKVKLAQSLAVGQRFILDVPDLDGVQGIYEAII
                     SVLETHRRSLDMNAISQAVSWLSQARQILALGTGGGSTICSQEIQYRLFRLGLPVVSQ
                     NDALMMRMMCSAVMPQDVVIVLSLGGYTPEIIESAAIARQYGARVIAITPAQTPLAEQ
                     VDLVLPLLVQESDYIFKPTPSRYAMLAMVDVLATELAMANKAQAKDRLRRIKLVLDSH
                     RGGANRQPLGD"
     gene            complement(12464..13933)
                     /locus_tag="ACHOQV_002526"
     CDS             complement(12464..13933)
                     /locus_tag="ACHOQV_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFP9630887.1"
                     /translation="MNSHIFLVGFIIYAIAMIWLGWFVSRNQKSGEDFLLGGRSLPLF
                     LTLGSTVATMVGTGSSMGAVGFGYSNGWAGMLYGVGGAVGILLVAWLFAPVRKLRFMT
                     MSEELSYYTGGSHLIKNIVGLMIFIASIGWLGAHILGGSMYLAWATGIDLTVAKLIIA
                     LAFAIYVIIGGYSAVVWTDTIQALILFFGFILMAILAVVHVGGWSAIEQAMDPKAMSL
                     FAIDKMGVLPALSLALVIGVGVLATPSYRQRIYSGKDVPSVRRSFVITGVLYLFFSIL
                     PAIIGMAAFTMNPNLENSNYAFLFATSFLPAILGLVVLIAGLSATMSSASSDAIAAVA
                     IMMRDVYTMITGRMPPEDKAITYSRWMLTFVIGLALVFALTSNDIISYITKMISMLMS
                     GLFICSILGRFWLRFNWQGALAALVSGMVMSIVVLMNADWLAYWGNPCIPSVLGSLVA
                     GVLVTLVTPASQVSREEALAIITNERENQSVVITKPEEA"
     gene            complement(14036..14425)
                     /locus_tag="ACHOQV_002527"
     CDS             complement(14036..14425)
                     /locus_tag="ACHOQV_002527"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP9630888.1"
                     /translation="MSIKRYGTEGGTGTGGQHLPFARAVEAGGWLYVSGQTPMKNGEV
                     VEGGIVEQSRLAIQNCVDIMTEAGYSLADVVHVKVILTDARYFQSFNKVFREFFGENP
                     PARICCVADLVVDCKVEVDVTCYNATR"
     gene            complement(14435..15877)
                     /locus_tag="ACHOQV_002528"
     CDS             complement(14435..15877)
                     /locus_tag="ACHOQV_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9630889.1"
                     /translation="MQVDWLIKNVTVIDGSGGPEFRGDVAITGDRIVDIAPALNVTAQ
                     QVIDGEGRVLAPGFIDVHTHDDINVIRIPEYLPKISQGITTVIVGNCGISAASAKMKG
                     EVPDPMNLLGEAEHFIYPTVESYAQAVEAARPSLNVGTLIGHTALRNNHMDDLFRPAT
                     VDEIAAMRADLRLALSQGALGLSSGLAYATAFQATTEEVMALAEELAGEKGVYTTHLR
                     SEFEPILDALDEAFRIGRHGKVPVVVSHHKCAGAKNWGRTKETLAFFDQMRQHQEIGC
                     DVYPYSASSSTLDLKQVTDEFDIVITWSQTHPQQAGKTLAQIAIDWQMSMLEAAKLLM
                     PAGAIYYNMDERDVRRVLSYPVSMIGSDGLPNDPMPHPRLWGAFPRVLGHYCRDEGLF
                     PLTTAIHKMTGLSASRFRLPQRGLVKVGYFADLVLFDPQTIRDVASFSDPKRPADGIE
                     AVMVNGVMSYGPDKHITGRAGRFLRRQTSH"
     gene            16078..17298
                     /locus_tag="ACHOQV_002529"
     CDS             16078..17298
                     /locus_tag="ACHOQV_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HFP9630890.1"
                     /translation="MKYHSEPLVPHKSALMQMPANLLAEDVCLPAAIIKKQALENNIT
                     WMQRYADARGVSLAPHGKTTMTPWIFQAQQAAGAWAIGVGSAWQAGAAMASGIQRVLM
                     VNQLVGKANMQLIAQLQRHYPTVDFICCIDSIENARALSAFFASQQQTLNVMIELGVP
                     GGRCGCRSRDAALTLAKQAAQLPGLRLRGLELYEGVLHGDDPQPQVEALLRDAAQLAC
                     DMASLVDGEFILTGAGTVWYDVVCNIWLAAEKPDNCRIVIRPGCYITHDTGIYDAAQQ
                     QLLARDPVACDLAGDLTSALELVAMVQSVPEADRAVVNFGKRDCAFDAGLPQPVAHYR
                     NGKSLAFDPQAIRSTGIMDQHCMLQLSANSDVQVGDILVFGTSHPCLTFDKWKTLLLT
                     DDDYNVLAELDTLF"
     gene            17566..19716
                     /gene="btsT"
                     /locus_tag="ACHOQV_002530"
     CDS             17566..19716
                     /gene="btsT"
                     /locus_tag="ACHOQV_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFP9630891.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            19766..19969
                     /gene="yjiX"
                     /locus_tag="ACHOQV_002531"
     CDS             19766..19969
                     /gene="yjiX"
                     /locus_tag="ACHOQV_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP9630892.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            19980..20936
                     /gene="yjiA"
                     /locus_tag="ACHOQV_002532"
     CDS             19980..20936
                     /gene="yjiA"
                     /locus_tag="ACHOQV_002532"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP9630893.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEVEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPSRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDKIRAAFAGLRK"
     gene            complement(20970..21272)
                     /locus_tag="ACHOQV_002533"
     CDS             complement(20970..21272)
                     /locus_tag="ACHOQV_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFP9630894.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(21287..21388)
                     /locus_tag="ACHOQV_002534"
                     /pseudo
     CDS             complement(21287..21388)
                     /locus_tag="ACHOQV_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
     gene            21585..22751
                     /locus_tag="ACHOQV_002535"
     CDS             21585..22751
                     /locus_tag="ACHOQV_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9630895.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIHEQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRDMVDREGMSYRITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            22815..24434
                     /locus_tag="ACHOQV_002536"
     CDS             22815..24434
                     /locus_tag="ACHOQV_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP9630896.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEVLGYGE"
     gene            24424..>24653
                     /locus_tag="ACHOQV_002537"
     CDS             24424..>24653
                     /locus_tag="ACHOQV_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9630897.1"
                     /translation="MGSKWIEVSLGEISEKIGDGIHGTPTYNNSGNYYFINGSNLIDN
                     SIKITETTKCVDHDEYLKHRKKLSNNTVLVSI"
BASE COUNT         6056 a         6487 c         6165 g         5945 t
ORIGIN      
        1 ttatagtgat aatattcatg cgataatttg tttgcgacga gagataattc gctttttaac
       61 accgagcttc tcccgataac caggattaat cagcgcaatt ttgatcttaa cctgccgcaa
      121 aacaaccgtg cttcatgata caaatggatt ctcattatct gaacaatacg cagcacgtct
      181 atgacaaagg gcgagttatg caaactgagc aacagcgagc cgtaacacgg ctttgtatcc
      241 agtgtggatt atttcttttg caacatggtg cggaaagcgc gttggttgat gagctttcct
      301 cacgactggg tcgggcgttg ggaatggaca gcgtcgaaag ttctatctct tcgaacgcca
      361 tagtgctgac aactattaaa gatgggcaat gcctgacatc gacacgtaaa aatcacgatc
      421 gcggcattaa tatgcatgtg gtgactgaag tccagcacat tgtgattctg gcggagcacc
      481 atctgctgga ttacaaaggc gtagagaaac gatttagcca aattcagcca ttacgttacc
      541 caagatggct ggtagcctta atggttggcc tttcttgcgc ctgtttctgt aaactcaata
      601 acggtggctg ggatggtgcc gtcatcacct tctttgccag tacggccgcg atgtatatcc
      661 gccagctgct ggcacaacgt catcttcatc cacagatcaa cttttgcctc accgctttcg
      721 ccgccaccac catttccggt ttgcttttgc aacttcccac tttcagcaat acccccacca
      781 tcgcgatggc cgccagcgtt ctgctgctgg tgccgggctt tccgttgatt aatgccgtcg
      841 ccgatatgtt taaaggccac atcaataccg gactggcacg ctgggcgatc gccagtctgc
      901 tgacactggc tacctgcgtc ggcgtggtga tggcactgac gatttggggg ctacgcggat
      961 gggtgtgatc gaatttctgt tagcgttggc gcaggatatg atcctcgccg ccattcctgc
     1021 ggtcggcttt gcgatggtgt tcaacgttcc cgtgcgggcg ttacgctggt gtgcgttgct
     1081 tggctcgata ggtcatggtt cccgaatgat cttaatgacc agcgggctga atattgagtg
     1141 gtcaactttt atggcttcta tgctggtcgg caccattggt attcaatggt cgcgctggta
     1201 tctggcgcat ccgaaagtgt ttaccgtggc ggctgttatt cctatgttcc ctggcatatc
     1261 ggcttatacc gcaatgattt cggcggtaaa aatcagccag ctaggttaca gcgaaccgtt
     1321 gatgattacc ctgttaacca actttcttac agcttcatcg attgttggtg cgttatccat
     1381 cggtctttcc attcctggat tatggttgta ccgcaagcgc cctcgcgtat aaaattgcct
     1441 cacttaaagc acgcggatga gagacagcct cctctcctcc gtgtgttact ataaaagtta
     1501 tctcccttct cgttcatcgt tccatatttg agaaacagta tgtcttccag agttttgacc
     1561 ccggacgtcg ttggtattga cgccctggta cacgatcacc aaaccgttct ggcaaaagct
     1621 gaaggcggtg tggttgccgt atttgctaac aatgccccgg cgttttatgc cgttacgcct
     1681 gcacgcctgg ctgaactgct ggcgctggaa gaaaagctgg cgcgtccggg aagcgatgtc
     1741 gctctggacg atcaactcta tcaggaaccg caagccgctc ccgttgctgt acccatgggg
     1801 aaattcgcca tgtatccgga ctggcaaccc gatgccgact ttatccgcct ggcggcgcta
     1861 tggggcgtgg cgctaagaga gccggtgacc accgaagaac tggcctcatt cattgcctac
     1921 tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc aacaaaaact ggcgcgcagc
     1981 ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac gagatgtgaa tacggtcagc
     2041 gaacctgaca gccaaattcc accaggattc agagggtaac gatgaaaaac gttggcgacc
     2101 tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa acctgcgttc aaaacgggtg
     2161 aagaactact ggcctggcag aaagaacaag gagcgatccg ctccgccgct ctcgaacgtg
     2221 aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc cggtattcgt ccactgcatc
     2281 agaactgctc ctttgagaac tatcgcgttg agtgtgaagg gcagatgaat gcgttaagca
     2341 aagcgcgcca gtatgtcgaa gagtttgacg gcaacatcgc cagcttcatc ttttctggca
     2401 agcccggaac cggcaaaaac catctggcgg cggcaatctg caacgagctg ctgctacgcg
     2461 gtaaatccgt attgatcatc accgtggccg atattatgtc ggcgatgaaa gataccttca
     2521 ggaatagcgg taccagcgaa gaacaactgc ttaacgatct gagcaacgtt gatctgctgg
     2581 tgatcgatga gatcggcgtg cagaccgaat cgaaatacga aaaagtgatc atcaaccaga
     2641 tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat gctgaccaac agtaatatgg
     2701 aagagatgac caagttgctg ggcgaacgcg tgatggaccg tatgcgcctg ggtaacagtt
     2761 tgtgggtgat cttcaactgg gatagctacc gtagccgggt aacgggtaaa gagtattaat
     2821 ccccgtttct ggcggtatac tgcgacacaa ttccaccttg agtaatgatg atgaaaactg
     2881 ttaaacatct tctgtgctgt gccattgccg ccagcgcatt aatttccacc ggggtgcatg
     2941 ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga acttggcaac caaaacagca
     3001 cgacacagga aggcggttgg tcgctcgcgt cattaactaa cttgcttagc agcggaaacc
     3061 aggccttaag cgcagataac atgaacaatg ccgcaggcat tctgcaatac tgcgcgaagc
     3121 aaaagctggc ttcggtaacc gacgccgaaa acatcaagaa tcaggtactg gaaaaactgg
     3181 gcctgaacag tgaagagcaa aaagaagaca ccaactatct ggatggcatt cagggtttgc
     3241 tgaaaaccaa agatggtcag caactcaatc tggataatat tggaacgact ccgctggcag
     3301 aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca ggggctgaac ttcatttcct
     3361 gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac gcggctctct cctgcccttt
     3421 tccctgctcc tggacggttt tccccctgtt acacgggaag cgaccgcgat caaaaaagtc
     3481 tgtttataaa ctaattccta acaacatcgc atttttttga cactagaatt gcagcaatat
     3541 ggcgacgcca ttacattgtg cttctcaaaa agcttaaacg ttggctaaac ggcccggttt
     3601 agtgaggatc gcccgttgtc agaactactc tctttcgccc tttttctcgc ctctgtgctg
     3661 atttacgcat ggaaagcggg acgtaacacc tggtggtttg cagccacatt aacggtactg
     3721 gggctatttg tcgttttaaa tatcaccctg tttgccagcg actattttac tggcgatggt
     3781 attaacgacg cggttctcta taccttaacc aacagcctga ccggtgctgg tgtcagtaaa
     3841 tacatcctgc ctggtatcgg cattgtgctg gggctgacag cggtgttcgg tgcgctgggc
     3901 tggatcctgc gccgtcgtcg tcatcatccg caccattttg gttacagcct gctggcactc
     3961 ttactggcgt tgggttcagt ggatgccagc ccggcatttc gtcagataac ggaactggtg
     4021 aaatcccagt cacgcgacgg cgacccggac tttgcggctt attataaaga gccgtcgaaa
     4081 actatccctg acccgaaact caacctggtt tatatctacg gcgaaagtct cgagcggacc
     4141 tattttgata acgaggcttt cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa
     4201 ggcctggatt tcagccacac gcagcagctg ccaggaacgg attacacgat tgcgggcatg
     4261 gtggcttctc agtgcggaat accgctgttt gccccctttg aaggcaacgc ctccgcttct
     4321 gtttccagct tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat
     4381 cagaactatt tcgtgcaggg cgcgaatctg cgttttgccg gtaaagatgt gttcctgaag
     4441 tcgcatggct tcgaccactt atatggctca gaagagctga aaagcgtggt ggccgacccg
     4501 cactatcgca acgactgggg attctacgac gataccgttc tcgacgaagc gtggaaaaag
     4561 tttgaagagc tttcccgctc aggtcagcga ttctcactgt ttaccctgac agtcgatacc
     4621 catcacccgg atggttttat ctcgcgtacc tgtaaccgca aaaaatatga ttttgacggt
     4681 aagccgaatc agtcattcag cgcggtaagt tgcagccagg agaatatcgc gacgtttatc
     4741 aacaaaatca aagcgtcacc gtggtttaaa gataccgtca tcgtcgtctc ttctgaccat
     4801 ttagcgatga acaacacggc gtggaaatac cttaataaac aggatcgcaa taacctgttt
     4861 tttgtcatcc gtggcgacaa gccgcagcaa gagacgctgg cggtgaagcg taacacgatg
     4921 gataacggcg cgacggtgct ggacattctc ggtggcgata actatctcgg acttggtcgt
     4981 agcagtttat ccgggcagtc aatgtcggaa atcttcctca atatcaaaga gaaaacattg
     5041 gcgtggaagc cggatatcat ccgcctgtgg aaattcccta aagagatgaa agagttcacc
     5101 atcgaccagc agaaaaacat gattgccttc tcgggtagcc atttccgttt gccgctactg
     5161 ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa gtgaatactc agcaccgctg
     5221 cgtttccagc tggccgattt cgctccacgc gacaatttcg tctgggttga ccgttgctac
     5281 aagatggcac aactttgggc tccggaactg gcactctcca ccgactggtg tgtctcgcaa
     5341 gggcagcttg gcggtcagca aattgtccaa catgttgaca aaacaacatg gaagggcaaa
     5401 acggcattta aagatacggt catcgacatg gcgcgttaca aaggcaatgt cgatacgctg
     5461 aagattgttg ataacgatat tcgctacaaa gccgacagtt tcatctttaa cgtcgccggt
     5521 gcgccagaag aggtgaaaca gtttagcggg atttcccgtc cggagtcgtg gggccgctgg
     5581 tccaacgctc agctgggcga cgaagtaaaa atcgagtaca agcatccgct gccgaagaaa
     5641 tttgacctgg tgattaccgc caaagcatac ggcaataacg ccagccgtcc tattccggta
     5701 cgcgtaggca atgaagaaca aactcttgtg ctgggcaatg aagtgaccac caccacgctg
     5761 catttcgata acccaaccga tgccgacacg ctggtaattg tgccgccgga acctgtctca
     5821 accaacgaag ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg catggtggaa
     5881 attaaagtgg tagaacgtga agggtaattc ctgactgatg tgtaggtcgc ataaggtgcg
     5941 acctacacgc cttctccggc ttacaacaca ctattaagaa acaaaaaaaa cagcgcccaa
     6001 cacttccccg gacgctgcat ggcaacttta ataatctcgc cttatcagaa agtaattaca
     6061 cccttaatca actcacgatt gttaatcaca tcacgctcgt aggtttctgc cagcgtggcg
     6121 aacgggtagc gatgggttaa catcatgtcg gcagtgattt tcccttccgc catcagtcga
     6181 ccgactttag cgaaatcttc cggcgtggcg ttgcggctac ccatcatcgt cgtttctttt
     6241 ttatggaatt ctggatcgga gaactgcaac tcacctttaa acagaccgac aaataccacc
     6301 gtgccgccgt gacgaatcag attcacggtg ttattcatcg catgttgatt acctgtcgcg
     6361 tcgatcactt tctgcgccag cgaaccacca aactgcgccc gcagctgcgc gtcaaaatct
     6421 tcggctgatg ggtccagcac aggcaactcc agacgcgttg ccacatgttc acggcgcgcc
     6481 ggactggtat ccgccaccgc taccagtgcg ccatcggctt tagcgattgc cgccgcgccc
     6541 agaccgattg gccccgcccc gaccaccagc acctgctcgc cgggagcaat ggctgcgcga
     6601 cgcaccgcat gagcgctaat agcgaaaggt tcaatcaatg ctgccgcctg cgggtcaata
     6661 ccgtctgcgg gcaaaatgtt cgccaccggc acgctcaaat actcactaaa accgccatcc
     6721 tgatgcacgc caatgactga aattttttca cagcaattgg tacgcccgct tttacacgcc
     6781 gggcattgct gacaggcaac ataagggatc acagcaactt gctgaccatt tttaagatca
     6841 gcaatatttt tacccagccc aacaatctcc ccacatattt catggcctaa aacgcgtgga
     6901 taactaaaaa atggttgatt tccaccccag gcatgaatat cggtaccgca aatcccgaca
     6961 gacttaattt ttattaatgc ttcattgtca cccggaatag gtatctcgcg ttgtttccag
     7021 actaattctt tcggctgctg gcaaattaaa acattcatcg tagacatgat tcggctctcc
     7081 attttttgtt gccttagata tcaatgaaaa tgaaaaatgg cagcggaacg gaaaatctgt
     7141 tttgtgaaaa cacacgcata aaaatgtttt aaatcgggtt ttaatggact cacaaggagg
     7201 aaaatatgag tcgttcacaa aatttacgcc acaatgtgat taaccaggtc attgatgata
     7261 tggcgcgggg tcatatccct tccccacttc catcgcaaag tgcgctggcg gaaatgtaca
     7321 acatcagccg cactacagtg cgtcatattc tcagccactt acgcgaatgc ggcgtcttaa
     7381 cgcaggttgg aaacgactat gtgattgcgc gtaaacctga ccatgatgac ggtttcgcct
     7441 gtactaccgc ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt ttcacgatga
     7501 taaaccagcg ccagttacgc ccaggggaaa ctttttctga actgcaactg gctcgggccg
     7561 cgggtgtcag cccggttgta gtaagagaat atcttttaaa attcggccgt tataatctta
     7621 ttcaaagcga aaagcgcggc cagtggagca tgaagcaatt cgatcaatcc tacgctgagc
     7681 agctgtttga gctacgcgaa atgctcgaaa cacattcact tcagcacttt ctcaacctgc
     7741 ctgatcatga tccccgctgg ctgcaggcga aaaccatgct cgaacgtcat cggttactgc
     7801 gtgacaacat cggcaatagc tttcgcatgt tctcgcaact tgacagagat tttcactcct
     7861 tactgctttc agctgccgac aatatctttt ttgatcaatc gcttgagatc atctcagtca
     7921 tctttcattt tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat atcattgccg
     7981 tcgatgagca tatgaccatt ctcagtgctc tgatttgccg cagtgacctt gatgccaccc
     8041 ttgcgctccg taaccatttg aattcagcta aacaatcaat gattcgatcc attaatgaga
     8101 acacgcgtta tgctcattaa gtacggatta aaaacagcaa actattagcg ggtgcacggt
     8161 tttatcaaaa gataaatacc gggaaattcc cgcttaccta tgctcacaat cagtcgacta
     8221 aaaatgtttt tgtgagcggt agtaaagtcc taaaacttta acctgccgtt aatgtcaacc
     8281 ttgcgtaatg cacaattacg taaacaatta aaattataaa tacaggagtc cggcgtggaa
     8341 aaagaaaata tcaccctcga tccgcgttct tcatttactc catcttcgtc ggcagatatt
     8401 cccgtgccac cagatggatt agttcaacgc agtacccgaa ttaaacgcat tcaaaccacc
     8461 gccatgttgt tattattttt tgcggcggta atcaattatc tcgaccgcag ttcgctgtcg
     8521 gttgcaaatt taacgattcg tgaagaattg ggattaagtg ctaccgaaat cggcgctttg
     8581 ctctccgtgt tttcactcgc ttacgggatt gcgcaacttc cttgcggccc actattggat
     8641 cgtaaaggcc cacgcctgat gctgggactg gggatgttct tctggtcact gttccaggcc
     8701 atgtctggca tggtgcacag ctttacgcag ttcgtgttgg tgcgtatcgg tatggggatt
     8761 ggtgaagcgc cgatgaaccc atgcggtgta aaagtcatta acgactggtt caacatcaaa
     8821 gagcgcggac gcccgatggg cttcttcaac gcagcttcta ctattggcgt tgccgtaagc
     8881 ccaccgattc tggcggcgat gatgctggtg atgggctggc gtgggatgtt tattaccatt
     8941 ggtgtgctgg ggatttttct cgccattggc tggtatatgc tctatcgcaa ccgcgagcac
     9001 gtagaactga ctgccgttga acaagcttac ctcaatgctg gtagcgtcaa tgcccgccga
     9061 gatccgctca gttttgccga atggcgcagc ctgttccgta accgcacaat gtggggaatg
     9121 atgatcggat tcagtggcat caactacact gcgtggctgt atctggcctg gcttcctggt
     9181 tacctgcaaa cagcctataa cctggattta aaaagcacag ggttgatggc ggctatccct
     9241 ttcctgtttg gggctgccgg gatgctggtc aacggttacg ttaccgactg gctggtcaaa
     9301 gggggaatgg ctccgattaa aagccgtaag atctgcatta ttgccgggat gttctgttct
     9361 gccgccttta cgctggtcgt accgcaagcg acaacatcca tgacagcggt tctgctgatt
     9421 ggcatggcac tgttttgtat tcactttgcc ggaacatcct gctggggctt gatccacgtc
     9481 gcagttgctt ctcgcatgac ggcgtcggtg ggcagtatcc agaactttgc cagcttcatc
     9541 tgcgcctctt tcgcgccgat cattactggt tttattgttg ataccaccca ctcattccgt
     9601 ctggcactaa tcatctgcgg ttgcgtcacc gcagcggggg cactggcgta catcttcctg
     9661 gttcgtcagc cgatcaacga cccgcgtaaa gattaaactc cctatgcccg ataacatttt
     9721 gcttatcggg catttttatg gcgattaaaa cgtttcccaa ttctcgccgc tatctgccac
     9781 ggccatttta cgcggcgtag ctggcgtcac ggtttttact acagccgatg tttcacgctg
     9841 ctgttgctgc tgttgctgaa tccggaacac tgccactgct tcggtcaggc gactggcctg
     9901 ctcttccagc gcggcggcgg cagcggcaga ttcttccacc agcgcggcgt tttgttgggt
     9961 tacccggtcc atctcagcaa ccgctaagcc aacctgatcg ataccacggc tctgctcatc
    10021 agaagcagaa gcaatttcgc ccataatgtc cgtcacgcgg gtcacggcac tgacaatctc
    10081 cgccattgtt tccccggcgc tttcgaccag cgtagagcca acatccactt tccccaccga
    10141 gtcttcaatc aggcttttaa tttcacgagc agcctgagcg ctacgctggg ccagattacg
    10201 cacttctccc gcaaccaccg caaaaccgcg cccttgctca cccgcgcgcg ctgcttcaac
    10261 cgccgcgttc aaagccagaa tattggtctg gaaggcaata ccgtcaatta cgctgataat
    10321 atcggcgatt ttctgcgaac tggtggagat atcgcgcata gtctgcacca cgttatccac
    10381 cactttgccg ccgcgttgcg ccgtttcgga agcacttaac gccaggtggc tggcctgacg
    10441 cgcattttcg gcgttctgct tcaccgttgc ggtcagttgc tccatgctgg ctgccgtctc
    10501 ttccagcgaa gcggcctgtt gctcggtgcg cgaagagaga tcgttattgc cggtggcgat
    10561 ttcgctggca ccgctataga tggcattggc cccgttgcgc acatcaccga cggtacgcat
    10621 cagctctccc tgcatatggc gcaaactctc tgccagttgc cccatctcat tagagccatc
    10681 cacctcaatc ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt caatcaggcg
    10741 attcattggc gcgaccagcg aggctttaat accgaaccag acggcgagga tgaccgccag
    10801 tacgacgatc atcacgccta ccagaatcca catcgcctgg ctgtaggagg cattgttatc
    10861 gctgacggcg atatcataga gccgatcgtt ttgctccatg taagccacat actgcttctc
    10921 gaaaccgtcc tgatatccct gggtcggttg atcaaagaac tcgttgattt tgcctgcacc
    10981 taacagttgg atcagctccg ccagcgcatt gtgataaata tcgtaattac gtttgatctc
    11041 tgacgctgcg gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat ccgcccagtt
    11101 tttttccgcc tgtttcagcg aaatactggc actctgcatc agctcagcaa cggttgaacc
    11161 gctaccaata ttattctgat ccatcatgta gcggataccc gcgcggttga gggtgttacg
    11221 cgtctgcaac aacgcaaccc agctgccatt cagcgtggat tgctgctggc gaatggtttg
    11281 taaaacagtg aaattttctt tgtcattctt taaggcatta aagaacagac cgcctgatgt
    11341 cagttgtaaa aggccaaaaa cggccaaaac cagcagtaag ctggtcacga tcttgatacg
    11401 ttttaacatg gtttctcttt ccactagaca gatacactgg ttgtcggcct ggtggtggaa
    11461 aactttatgc tgtggaaaag cgtcggcact gcttttgttg cagcaaactt gcgttttttg
    11521 tgagggtcac actctatagc ggcagtttat ggtacaaagt atcaatcttc ttcgaaccag
    11581 tgatactcct atggcttata gcatcgatat catctcctgc attaccgaac ggtttatggc
    11641 gttaacagcc actgaaaagc gtatcgcaca atttattctt gatgacgtct ctgccgcagt
    11701 agagttgcca attgctgagc tggcacggtt aacgcaaacc agtcaggcgt cggtgacgcg
    11761 ttttgcccgt gcactggggt gcaaagatgt ccgcgagttg aaagtcaaac tggcgcagtc
    11821 actggctgtc gggcagcggt ttattctcga tgtaccggat cttgacggtg ttcaggggat
    11881 ctacgaagcg attattagcg tgctggagac gcatcgtcgt tcgctggata tgaatgccat
    11941 cagccaggct gtaagctggt taagtcaggc gcggcaaatt cttgcgttag gaactggcgg
    12001 cggttccact atctgttcac aggagattca gtaccgttta ttccgtctgg ggctaccggt
    12061 ggtcagtcag aatgatgccc tgatgatgag aatgatgtgc tcggcagtca tgccgcagga
    12121 tgtggtgatt gtgctttcgc tgggcggtta tacaccggaa atcatcgaaa gtgcggcaat
    12181 cgcccgtcag tatggcgcgc gtgttattgc cattacgcca gcacaaacac cgctggcgga
    12241 acaggtggat ctggtattgc cgttactggt tcaggaaagt gattacatct ttaagcccac
    12301 cccttcccgc tacgcgatgt tagccatggt cgatgtactg gcaacagaac tggcaatggc
    12361 aaataaagcg caggcgaaag atcgcctgcg gcggataaaa ctcgtgttag acagccaccg
    12421 tgggggcgct aaccgccagc cacttggcga ttagcattgt cggttatgct tcttcaggtt
    12481 tggtgatcac gacactctgg ttttcacgct cattggtgat aatagccaaa gcttcttccc
    12541 ggctgacctg acttgctggc gttaccaacg tcaccagtac gccagcgacc aggctgccca
    12601 gcactgacgg aatacacggg ttaccccagt aagccagcca gtcggcattc atcagcacca
    12661 caattgacat caccataccg ctcactaacg ccgccagcgc gccctgccag ttaaagcgta
    12721 gccagaagcg accgagaatc gagcagataa acagcccgga catcagcatc gaaatcattt
    12781 tggtaatgta gctgatgatg tcattagagg tcagagcaaa gaccagcgcc aggccaataa
    12841 caaaggtcaa catccagcgt gagtaagtaa tggccttgtc ctccggcggc attctgccgg
    12901 taatcatggt gtagacgtcg cgcatcataa tcgccaccgc cgcgatggca tcggagctgg
    12961 cagaagacat ggtggctgaa agcccggcaa tcagcaccac cagcccaaga atggcaggta
    13021 agaaactggt cgcgaacaag aaggcgtagt tgctgttttc cagatttggg ttcatggtga
    13081 acgccgccat gccgataatg gcaggcaaaa tcgagaagaa cagatataac accccagtaa
    13141 taacaaacga acggcgtaca gagggaacat ctttacccga gtaaatacgc tggcgataag
    13201 acggtgttgc cagcacacct acgccaataa ccagtgccag cgaaagcgca ggcagcacgc
    13261 ccattttatc aatcgcaaac aggctcatcg cttttgggtc catcgcctgc tcaatcgcac
    13321 tccagccgcc aacgtgaaca acggcaagaa tagccattaa tataaagcca aagaacagaa
    13381 ttagggcctg aatagtatct gtccacacta ccgccgaata accgccgata ataacataaa
    13441 tcgcaaaagc taaggcgata attaattttg ccacagtaag atcaataccc gtcgcccagg
    13501 caagatacat acttccacct aatatatgcg cccccagcca gccaatagag gcaataaata
    13561 tcatcaggcc gacaatattt ttaattaaat gactaccgcc agtataataa gagagttctt
    13621 cactcatggt cataaaacgt aatttacgta ccggtgcaaa cagccacgcc accagcaata
    13681 tacctacggc tccaccgacg ccataaagca ttccggccca gccattgctg tagccaaagc
    13741 ccactgcccc catactggaa cccgtgccaa ccatcgtggc aacggttgac cccagcgtca
    13801 gaaacagcgg cagcgaacgc ccgcccagca gaaaatcttc tccgcttttc tgattacgag
    13861 aaacaaacca accaagccaa atcatggcga tggcataaat aataaagccg actaaaaaga
    13921 tatgactgtt cataattcac atcctgccag tagagagaaa ttaatgtaag agttcccgga
    13981 gtgctgaatg tattcagcac tcaattgcgg tctgtttttt taattcttaa atttattagc
    14041 gggtcgcgtt ataacaggtc acatcgactt cgactttaca atcgacaacc agatcagcca
    14101 cacaacaaat acgtgcaggc ggattctcgc caaaaaattc tctgaatact ttattaaatg
    14161 actggaaata gcgggcgtcg gtcaggatca ctttcacgtg aaccacatcc gccaggctat
    14221 aaccggcttc agtcatgatg tcgacacagt tttggatcgc cagtcgtgac tgttcaacga
    14281 ttccgccttc taccacttca ccgtttttca tgggtgtttg cccggagacg tacagccagc
    14341 cgcccgcctc aaccgccctg gcaaaaggca aatgctgccc gccagtgcct gttccacctt
    14401 ccgtaccgta acgtttaatg ctcatattca ctccttaatg tgaggtctgc cggcgcagga
    14461 agcgtcccgc acggcctgta atatgtttat cgggaccata gctcataacg ccgttcacca
    14521 tcacagcctc aatgccatct gccgggcgtt tgggatcaga aaagctggca acatcacgaa
    14581 tggtttgtgg atcgaacaac accaggtccg caaaataacc gactttcacc agtccacgct
    14641 ggggcaggcg aaaacggctg gcagaaagcc cggtcatttt gtggatagcc gtggtcagtg
    14701 ggaataagcc ttcatcacgg caatagtggc ccagcacgcg agggaaggcg ccccataaac
    14761 gtggatgcgg catgggatca ttgggcaggc cgtcagagcc aatcatactg accggataac
    14821 tcaacactcg gcggacgtcg cgctcgtcca tgttgtaata gatagccccg gcaggcatta
    14881 gcagcttcgc ggcctccagc atactcatct gccagtcgat ggcgatttgc gccagtgttt
    14941 tcccggcctg ctgcggatgt gtttgtgacc aggtgatcac aatatcgaat tcgtcggtga
    15001 cttgtttgag atccagcgtt gaagagctgg ctgagtaagg gtagacatcg cagccaattt
    15061 cctggtgttg acgcatttgg tcaaaaaagg ccaacgtttc ttttgtccgc ccccagtttt
    15121 tcgccccggc gcatttatgg tgggaaacca cgacaggcac tttgccatga cgcccaatac
    15181 gaaacgcctc atccagggca tcgagaatcg gttcaaattc agaacgcaga tgggtggtat
    15241 aaacgccctt ctcaccggct aattcttccg ccagcgccat gacttcttcg gtagtcgcct
    15301 gaaatgccgt tgcataggcc agcccggaac ttaaccccag cgccccctga ctcaacgcca
    15361 gacgtaagtc ggcacgcata gcggcaattt catccaccgt cgccgggcga aacaggtcgt
    15421 ccatatggtt attacgcagc gccgtatgac caatcagcgt gccaacgttc agtgacggcc
    15481 tggctgcttc cactgcctgg gcatagcttt caacggtagg ataaataaag tgctctgctt
    15541 cccccaacag attcatcggg tcaggaactt cacctttcat tttcgccgac gccgcactaa
    15601 tcccgcagtt accgacaatc accgtagtga tcccctggct gatctttggc agatattccg
    15661 ggatgcgaat aacgttgata tcgtcatggg tatgaacgtc gataaacccg ggtgccagca
    15721 cccgcccttc cccgtcaatg acctgctgcg ccgtaacgtt aagcgcaggg gcaatatcga
    15781 caatccgatc acctgttatt gccacgtcac cgcgaaattc agggccgccg ctaccgtcga
    15841 taacggtcac atttttgatt aaccagtcaa cctgcatgtt cgtcactcca tttctgcaat
    15901 ggtgacaagt taaccattta caaggacgaa aaacagtgga aaactacgca aaacccagat
    15961 gattttatga tacttttatt cataaataat aagataaata aactaaatat ttatatttca
    16021 tttagttaca aatgatactc acatccgata cggtgaaaaa cttaaaaggt aaatgttatg
    16081 aaataccact cagaacctct ggttccccat aaatctgccc tgatgcaaat gcccgcaaat
    16141 ctccttgccg aagatgtctg tttacctgcc gcgatcatta aaaagcaggc cctggagaac
    16201 aacattacgt ggatgcaacg ctacgctgac gcgcgcggcg tttcactggc accgcacggt
    16261 aaaaccacca tgacgccgtg gatttttcag gcgcagcagg cggctggtgc ctgggcaata
    16321 ggtgtcggta gcgcatggca ggctggtgcg gcaatggcga gcggtattca gcgagtgctg
    16381 atggttaacc agctggtcgg caaggcgaat atgcagttga ttgcccagtt gcaacgacat
    16441 tacccgacgg tcgattttat ctgctgtatc gacagcattg agaacgcccg cgctttgtct
    16501 gccttttttg ccagtcagca acagacactg aacgtgatga ttgagttggg tgtccccggc
    16561 gggcgctgtg gttgccgctc aagggatgcc gcactgacac tggctaaaca ggcagcccaa
    16621 ttaccggggt tacgcctgcg ggggcttgag ttatatgaag gcgttttaca cggtgacgat
    16681 ccacaaccgc aggtagaagc tctgctgcgg gatgccgcac aactggcctg tgatatggca
    16741 agtctggttg atggcgagtt tattctcacc ggagcaggca cggtctggta tgacgtggtg
    16801 tgcaatatct ggctggcggc agaaaagcct gataactgcc gcattgttat tcgccctggc
    16861 tgctacatca ctcacgacac cgggatctac gacgcggcac aacaacagtt gcttgcccgt
    16921 gatcctgtgg cctgcgatct ggcaggcgat ctcacatctg cgctggaact ggtcgccatg
    16981 gtgcaatcgg tgccggaagc tgatcgtgcg gtggtaaatt ttggtaagcg tgattgcgcc
    17041 ttcgacgccg gactgccgca accagtcgcc cactaccgca acggcaaatc actggcattt
    17101 gatccacagg cgattcgcag tacaggtatt atggatcagc actgcatgtt gcaactgtca
    17161 gctaatagcg acgtgcaagt gggggatatt ctggtgtttg gcacatcgca tccgtgcctg
    17221 accttcgaca aatggaaaac gttgttattg actgatgacg actacaacgt actggcagaa
    17281 ttagacactc tcttctaaga acgccccttc ggttaattat cgaaggggca tttttattgt
    17341 gatctcaatc acatatccac ccgacgaata attcttgccc aaccgctcac cgccattcct
    17401 gaccgcttac atccctaaaa taaccactca gttatttacc ttactttacg cgcgcgtaac
    17461 tctggcaaca tcactgcagg atagcggtca atttacctcc tcaaacgcaa cgcaaaccta
    17521 gaacggcttc ggccaactat taatcaatac atgccaggtt ttactatgga tactaaaaag
    17581 atattcaagc acataccctg ggtgattctc ggaatcatcg gtgcattctg cctcgcggta
    17641 gttgcattac gtcgggggga gcacgtcagt gccctgtgga tcgtggtcgc ctctgtgtcg
    17701 gtgtatctgg tggcttatcg ctactacagt ctgtacatcg cccagaaggt gatgaaactc
    17761 gaccccacgc gcgcgacgcc tgcggttatt aacaacgacg gtctgaacta tgttccgacc
    17821 aaccgttacg tgttgtttgg tcaccacttc gccgctatcg ccggtgctgg tccgctggtt
    17881 ggtccggttc tcgccgcgca gatgggctac ctgcctggca cgctgtggct gctggcgggg
    17941 gtagtgctgg ccggtgcggt acaggacttt atggtgctgt ttatctcctc tcgccgtaac
    18001 ggcgcatctc tcggtgagat gatcaaagaa gagatgggac cagtaccggg gactatcgcg
    18061 ctgtttggct gtttcttaat catgatcatc atccttgccg tcctggcgct gattgtggtt
    18121 aaagccctgg ccgaaagtcc gtggggtgtc ttcaccgttt gctcaaccgt accaattgcg
    18181 ctgtttatgg gtatctacat gcgctttatc cgtccggggc gtgtgggtga agtctctgtc
    18241 attggtatcg tgctgctggt tgcctctatc tacttcggtg gcgtgattgc tcacgatccg
    18301 tactggggtc cggcactgac ctttaaagac accaccatta ccttcgcgct gattggctat
    18361 gcgtttgttt ccgcgctgct gccggtgtgg ctgatcctcg caccacgcga ctatctggca
    18421 accttcctga aaatcggcgt tatcgtcggc ctggcgctgg gtatcgtggt gctgaacccg
    18481 gaactgaaaa tgcctgcgat gacccagtac attgacggta ctggcccgct gtggaaaggc
    18541 gctctgttcc cgttcctgtt catcaccatc gcctgtggtg cggtatctgg cttccacgcg
    18601 ctgatctctt ccggtacgac gccgaaactg ctggctaacg aaaccgacgc gcgtttcatt
    18661 ggctacggcg caatgctgat ggagtccttc gtggcgatta tggcgctggt tgctgcgtcc
    18721 atcatcgaac cgggtcttta cttcgcgatg aacaccccgc ctgctggcct tggcatcacc
    18781 atgcctaacc tgcatgaaat gggtggcgag aacgcgccga tcatcatggc gcagttgaaa
    18841 gacgttaccg cacacgcggc ggcgaccgtc agctcctggg gcttcgtgat ttcgccagag
    18901 cagatcctgc aaaccgcgaa agacattggt gaaccttctg tcctgaaccg tgcaggtggt
    18961 gcgccgacgc tggcggtagg tatcgcgcac gtgttccaca aagtgctgcc gatggctgac
    19021 atgggcttct ggtatcactt cggtattctg ttcgaagccc tgttcatcct gaccgcgctg
    19081 gatgcgggta cccgttctgg ccgctttatg ctgcaagacc tgctgggtaa cttcatcccg
    19141 ttcctgaaaa aaaccgactc tctggttgct ggtatcatcg gtactgcggg ctgtgtgggt
    19201 ctgtggggct acctgctgta tcagggcgtg gtcgatccgc tgggcggcgt taagagcctg
    19261 tggccgctgt tcggtatttc caaccagatg ctggcagccg tagcactggt actgggcacc
    19321 gttgtgctga ttaagatgaa gcgcacccaa tacatctggg taactgttgt tccggctgta
    19381 tggctgctta tctgcaccac ctgggcgctg ggcctgaaac tgttcagcac caacccgcag
    19441 atggaaggct tcttctacat ggcaagccag tacaaagaga agattgctaa cggtactgac
    19501 ctgacggcac agcagattgc caacatgaac cacatcgttg tgaacaacta cactaacgcg
    19561 ggtctgagta ttctgttcct gattgtggtg tacagcatca tcttctacgg tttcaaaacc
    19621 tggcttgcgg tgcgtaacag cgacaaacgt actgacaaag aaactccgta cgttccaatc
    19681 ccggaaggcg gcgtgaagat ctcttcgcac cactaaccgt gtttagcccc gcttcggcgg
    19741 ggctttgttc ttatcagagt gaactatgtt tggtaactta ggacaggcaa aaaaatatct
    19801 cggccaggcg gcgaagatgt tgattggcat tccagactac gacaactacg tcgagcatat
    19861 gaagaccaac catcccgaca agccgtacat gagctatgaa gaattcttcc gcgaacgcca
    19921 gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc tgttgttaat ggagagagca
    19981 tgaacccgat tgcagttacc ctactcaccg gttttttagg cgcgggcaaa accaccctgc
    20041 tgcgccatat tcttaatgaa cagcacggct acaaaattgc cgtgattgaa aacgaattcg
    20101 gcgaagtctc cgttgatgat caactgattg gcgaccgcgc cacgcagatc aaaacgctga
    20161 ccaacggctg catctgctgt tcgcgctcca acgagctgga ggacgcgcta ctcgacctgc
    20221 tggacaatct cgacaagggc aatattcagt tcgaccgtct ggtcattgaa tgcaccggca
    20281 tggccgatcc cggcccgatt attcagacct ttttctccca tgagatttta tgccagcgtt
    20341 acctgctgga cggcgtgatt gcgctggtgg atgcggtaca tgccgatgag cagatgaacc
    20401 agttcaccat cgcccagtcc caggtgggct acgctgaccg tattctgctg actaaaaccg
    20461 acgtcgcagg cgaagtagag aaactgcgtg aacgcctggc gcgcatcaac gcccgcgcac
    20521 cggtctacac cgtcacccac ggtgacatcg acctgggcct gttgttcaac accaacggtt
    20581 ttatgctgga agaaaacgtc gtcagcacca aaccgcgttt ccactttatc gcggataaac
    20641 aaaacgatat ttcgtcgatt gtggtggaac tggattaccc ggtagatatc agcgaagttt
    20701 cccgcgtgat ggaaaacctg ctgctggaat cggcggataa actactgcgt tacaaaggga
    20761 tgctgtggat tgacggcgaa cctagccgcc tgctgttcca gggcgtccaa cgcctctaca
    20821 gcgccgactg ggaccgcccg tggggcgatg aaacaccgca tagtacaatg gtgtttatcg
    20881 ggattcagct gccggaggat aagattcggg cggcgtttgc ggggttgagg aagtaatttt
    20941 agatgatggc cgccatgtgg cggcctgaat tattttttat tctgatcgcg cagcatcgct
    21001 tcacgtaaaa ttgcgttcag gcgagtttga tagcctttac ctggtcgctt caaccattcc
    21061 atcacatcag catcaatacg caccgaagcc tgagttttca atggccgata aaacttaccg
    21121 cgttctgcgt tagaccattg ctcatcgctg gaaggtggga tatcactgta atcaatatct
    21181 tcgtcggatt tattcgccag cgccttcagt tcggcctcat gctgcacatc gagtgtagag
    21241 gaggtccctc gtttatgttt aaccatgctc atagcgattc cgttcgttgt ggatcatcaa
    21301 acactaacac cgcatcttca aagcggatgc catgtttcac ccgattacct ttggccttat
    21361 ttgcatccca ttcaaactcc attggcatag ttgccatccc taattgtata tacaaaaatg
    21421 tatgtacaat tattctgcaa ttatagcgtt gatgttggaa atagcatcac gtttcgaata
    21481 atcaaccatg aaattgacaa tgtgtgagtt ggacttgatt catccaatag aatttctcgc
    21541 ctgacatggc acactaaaca cctcgctgtc gttaagggat taccgtgaaa aactacaaag
    21601 tcattacccc gctttttcct acctatgcgc aagttaaggc tatgatgaaa gccgtctcag
    21661 gctattccct gaaagcggtg cgcaatatga ttactgccat tcacgagcaa accggaacgc
    21721 cgcaaaaacc cgttgactgg tcggaacctg acctgtggat cagcgaacgc ttaacgggtg
    21781 aagatgccga catcgcccgt cgtatttggg ataccgataa tcacatattg aacccgcgcc
    21841 acagctacgg ctgctactta ttcctcaact atccccagtt tgagctgatg gaaagcacgc
    21901 cagacgatac ctggcagccc acctcgcgcg ggcaaaaatt cctgcaagat gacgaaaaaa
    21961 cgctgcgttc actggacgat caggaaggca ttctgcaact tcttgaacta ttagctggcc
    22021 gcgaaatgtc gcgccgcgcc gatttattgc cggaatggca ggcctttttg catcagcatt
    22081 cgaaattcgc gtctgcaagt tcagtgaaaa gtacgctcta ctcccgtctc tataacctta
    22141 tcgaccgtga catggttgac cgtgaaggca tgagctatcg catcaccgat accgggcgcg
    22201 cctggcttgg aaaagcgttg ccggcacgaa aaagcgatcc acgtaaagag ctgctggaag
    22261 cggtgaaacg ctacaacgag cagcaaaaag agctgctgcg cgaacaaatc agcacgatga
    22321 acccgtacaa atttgaacaa ctggttggtc aattactgga agcaatgggc tacgagcagg
    22381 tcgaagtgac caaagcctct ggcgacaaag gtgtggacgt gattggccag gtgcaggtcg
    22441 gcatcaccac tattaccgaa gtggtgcagg tcaaaagaat gcagaacact atcactcgcc
    22501 catatatcga ccagttgcgc ggggcgttgc cttaccacaa agccattcgc ggtacactca
    22561 tcaccaccgg gaaatttgcg gcgaaatgtg cagaagcggc gctctttccg ggtgccgcgc
    22621 ccattacgct tatcgacggc gatcgtttac tggaactgtt aatcgaaaat aacgtcggca
    22681 ttcgccgcag caatgcagtg gaattgctgg atgtggattt gcagttgttt gatgagctgg
    22741 agatggatta aagggcgaaa tccgccgcct ctaccattac aatagcgacc caatttatct
    22801 gaacagagtt catcatggcc aaagcaccaa ctaaaaaagc caaagcaaag aaaggctttg
    22861 aagaaacatt atgggataca gcaaaccagc ttcgcggtag cgttgagtcc tccgaataca
    22921 agcacgtggt gttgagcctc gtgttcctga aattcatcag cgataagttt gaagcacgcc
    22981 gcaaaaaaat gattgccgat gggcaagcag acttccttga gatggaagtg ttctaccagc
    23041 aggacaacat tttctacctg ccggaagaag cgcgctggtc ttttatcaaa caaaatgcca
    23101 aacaggacga tattgcggtc cgtattgaca ccgccctctc gaccatagag aaacgtaacc
    23161 caacgctgaa aggcgcgctg ccagacaact acttcagccg tcagaatctg gaaaccaaaa
    23221 aactggcgtc actgattgat accatcgaca acatcgaaac gctggcacac gaaactgacg
    23281 ttgaaacgtt atcgaaagaa gacctggtcg gacgcgttta cgaatacttc ctcggtaagt
    23341 ttgccgcaac tgaaggcaaa ggcggtggtg agttctacac gcccaaatgt gtggttacgc
    23401 tgttaactga aatgctcgaa cccttccagg gcaaaattta tgacccgtgc tgcggctcgg
    23461 cagggatgtt cgtgcagtcg gtgaagtttg tcgagagcca tcagggtaaa agtcgtgata
    23521 tcgcgctgta tggtcaggaa ctgacagcca cgacgtataa actggcaaaa atgaacctcg
    23581 ccattcgcgg tctttcagct aacctcggcg aacgaccggc agacaccttc tttagcgacc
    23641 agcacccgga cctgaaagcc gactacattc tggcgaaccc gcccttcaac ctgaaagact
    23701 ggcgtaatga agccgaatta accaaagacc cacgttttgc tgggtatcgt atgccgccaa
    23761 ccggtaacgc caactacggc tggattttgc atatgctctc caagctgtcg gctaacggca
    23821 cagcaggttt tgtgctggca aacggttcga tgagttctaa caccagcggt gaaggcgaga
    23881 tccgtgcaca gatgatcgaa aatgatctga ttgactgtat gatcgcccta cctggacagt
    23941 tgttctacac cacgcagatc ccggtgtgtt tatggtttat gaccaaatcg aaggctgccg
    24001 atccggccaa aggttatcgt gatcgtcagg gcgagacgct gtttattgat gcgcgtaacc
    24061 tcggcaccat gattagccgc acaactaaag agttaacggc ggaagatatt gccaccatcg
    24121 ccgataccta ccatgcatgg cgcagtacgc cggaagaact ggctgcacgg attgcgcgtg
    24181 gtgacagcaa gctggaaaaa tatgaagacc aggctggctt ctgcaaagtt gcgaccctgc
    24241 aagacattaa agataacgac tacgttctga caccgggccg ctatgtaggt gcagccgagc
    24301 aggaagagga cggcgtggca tttgagacga aaatgcgcga attgtcgaag acgttgtttg
    24361 agcagatgaa gcaggcggaa gaactggatc gtgcgattcg ccagaatctg gaggtgctgg
    24421 gttatgggga gtaagtggat agaagtatca ctgggcgaga tttctgaaaa aattggagat
    24481 ggaatccacg gaacgccaac ttataacaat tcaggtaact attactttat taatggaagt
    24541 aatttaattg ataattcaat aaagataact gagacaacta aatgtgttga tcatgatgag
    24601 tatttaaaac accgtaagaa attgagtaat aataccgttt tagtttcaat aaa
//
LOCUS       DAZKGF010000068        24601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZKGF010000068 DAZKGF010000000
VERSION     DAZKGF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.068"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(135..326)
                     /gene="yfgG"
                     /locus_tag="ACHOQV_002538"
     CDS             complement(135..326)
                     /gene="yfgG"
                     /locus_tag="ACHOQV_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFP9630898.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            344..496
                     /locus_tag="ACHOQV_002539"
     CDS             344..496
                     /locus_tag="ACHOQV_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630899.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     YVIMMS"
     gene            678..2921
                     /gene="pdeF"
                     /locus_tag="ACHOQV_002540"
     CDS             678..2921
                     /gene="pdeF"
                     /locus_tag="ACHOQV_002540"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFP9630900.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQRALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEISQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2960..4501)
                     /gene="ppx"
                     /locus_tag="ACHOQV_002541"
     CDS             complement(2960..4501)
                     /gene="ppx"
                     /locus_tag="ACHOQV_002541"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFP9630901.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4506..6572)
                     /gene="ppk1"
                     /locus_tag="ACHOQV_002542"
     CDS             complement(4506..6572)
                     /gene="ppk1"
                     /locus_tag="ACHOQV_002542"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFP9630902.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6743..7381)
                     /gene="purN"
                     /locus_tag="ACHOQV_002543"
     CDS             complement(6743..7381)
                     /gene="purN"
                     /locus_tag="ACHOQV_002543"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP9630903.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7381..8418)
                     /gene="purM"
                     /locus_tag="ACHOQV_002544"
     CDS             complement(7381..8418)
                     /gene="purM"
                     /locus_tag="ACHOQV_002544"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP9630904.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8743..9369
                     /gene="upp"
                     /locus_tag="ACHOQV_002545"
     CDS             8743..9369
                     /gene="upp"
                     /locus_tag="ACHOQV_002545"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP9630905.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9455..10744
                     /gene="uraA"
                     /locus_tag="ACHOQV_002546"
     CDS             9455..10744
                     /gene="uraA"
                     /locus_tag="ACHOQV_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFP9630906.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10839..11540
                     /gene="hda"
                     /locus_tag="ACHOQV_002547"
     CDS             10839..11540
                     /gene="hda"
                     /locus_tag="ACHOQV_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFP9630907.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11646..12005)
                     /gene="arsC"
                     /locus_tag="ACHOQV_002548"
     CDS             complement(11646..12005)
                     /gene="arsC"
                     /locus_tag="ACHOQV_002548"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFP9630908.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12026..13489)
                     /gene="bepA"
                     /locus_tag="ACHOQV_002549"
     CDS             complement(12026..13489)
                     /gene="bepA"
                     /locus_tag="ACHOQV_002549"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFP9630909.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13702..14763
                     /gene="yfgO"
                     /locus_tag="ACHOQV_002550"
     CDS             13702..14763
                     /gene="yfgO"
                     /locus_tag="ACHOQV_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9630910.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14862..15332)
                     /gene="bcp"
                     /locus_tag="ACHOQV_002551"
     CDS             complement(14862..15332)
                     /gene="bcp"
                     /locus_tag="ACHOQV_002551"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFP9630911.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(15332..15904)
                     /gene="gcvR"
                     /locus_tag="ACHOQV_002552"
     CDS             complement(15332..15904)
                     /gene="gcvR"
                     /locus_tag="ACHOQV_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFP9630912.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            16050..16928
                     /gene="dapA"
                     /locus_tag="ACHOQV_002553"
     CDS             16050..16928
                     /gene="dapA"
                     /locus_tag="ACHOQV_002553"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP9630913.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASMKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16945..17979
                     /gene="bamC"
                     /locus_tag="ACHOQV_002554"
     CDS             16945..17979
                     /gene="bamC"
                     /locus_tag="ACHOQV_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFP9630914.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            18192..18905
                     /gene="purC"
                     /locus_tag="ACHOQV_002555"
     CDS             18192..18905
                     /gene="purC"
                     /locus_tag="ACHOQV_002555"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP9630915.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            19073..19936
                     /gene="ypfJ"
                     /locus_tag="ACHOQV_002556"
     CDS             19073..19936
                     /gene="ypfJ"
                     /locus_tag="ACHOQV_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFP9630916.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19951..21966
                     /gene="tmcA"
                     /locus_tag="ACHOQV_002557"
     CDS             19951..21966
                     /gene="tmcA"
                     /locus_tag="ACHOQV_002557"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFP9630917.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATYVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPQTPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENAIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDIVLSADDWLELAGFAFAHRPLLTSLGCLMRLLQTSEMALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALYALDDVRTERLRDRITQWQFFH"
     gene            22040..22738
                     /gene="ypfH"
                     /locus_tag="ACHOQV_002558"
     CDS             22040..22738
                     /gene="ypfH"
                     /locus_tag="ACHOQV_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9630918.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     TATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22819..23019)
                     /gene="ypfN"
                     /locus_tag="ACHOQV_002559"
     CDS             complement(22819..23019)
                     /gene="ypfN"
                     /locus_tag="ACHOQV_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFP9630919.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVNHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(23047..24174)
                     /gene="dapE"
                     /locus_tag="ACHOQV_002560"
     CDS             complement(23047..24174)
                     /gene="dapE"
                     /locus_tag="ACHOQV_002560"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFP9630920.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(24178..24534)
                     /gene="yffB"
                     /locus_tag="ACHOQV_002561"
     CDS             complement(24178..24534)
                     /gene="yffB"
                     /locus_tag="ACHOQV_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFP9630921.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         5788 a         6098 c         6459 g         6256 t
ORIGIN      
        1 caggataaac atcagtcagg acaaaaacgt aaaatttaag atgaaaaaat atctgccgct
       61 ttgataatcc caaagagcaa aagcatccga cagcgaggtg ccgttatgtt ctgtgagagt
      121 ggagaaggta accgctaacg ttgtactggt gattcgacgg agagtgtgga ttgctgagct
      181 tcttttttgc tctgatggtg ctgccaggca ccgacggacg agtagataaa acggccaaag
      241 aagaagataa agctgatgag cagtacgata cgggtcatgc gactgttaaa tcggtgtcgt
      301 tttcgcatac tggttgcctg actcacaaaa ggttccttga agtatgtccc acgccgtgga
      361 cggtacttac attaaggcac aacaggacaa aatggtcaat tcttcgttat gtaaaaaagc
      421 gtcagtggat acatatttta atgttatgga agttaattta attatttaca atgattatgt
      481 aataatgatg agttaacatg ataaacgtaa taattcatta atcagggtta ttttgtttga
      541 tatatatcaa ttggaacttt catatgacac ttagaatcat tgctctctat agtgatgaat
      601 aatcatcatt cgaagtcagg tgggatgtct gtctgaatac acctccttca ggatgtgggg
      661 gattctgctg agcatctatg aaactgaatg caacttatat aaaaatacgt gataaatggt
      721 gggggcttcc gctgttcctg ccttctttaa tcttgcccat tttcgcccac attaatactt
      781 tcgcgcatat ttcttccggt gaagtttttc tcttttatct gccactggca ctgatgatca
      841 gcatgatgat gtttttcagc tgggcggcat tgccagggat cgccttaggg atttttgtcc
      901 gcaaatatgc agagctgggt ttttacgaaa cgctctcatt aacggctaat tttattatca
      961 ttatcattct ctgttggggc ggttacaggg tttttactcc ccggcgtaac aacgtttcac
     1021 atggtgatag ccgtttaatt tcccagcgac tattctggca gattgtgttt cctgcaacgc
     1081 tgtttctgat acttttccag tttgctgcgt ttgttggatt actggcgagc agagaaaatc
     1141 tggtcggcgt catgcccttt aacctcggga ccttaatcaa ttatcaggcc ttgctggtgg
     1201 gtaatctgat tggtgtcccg ctgtgctact tcatcattcg ggtggtgcga aatccgtttt
     1261 atttacgtag ctattattcg caattaaaac agcaggttga tgccaaagtt accaaaaaag
     1321 agttcgcaat ctggctactg gcattaggtg ctttactatt gctgttatgc atgccgttaa
     1381 atgaaaaaag cacgattttt agcaccaatt acaccttgtc attattgctg cccctgatga
     1441 tgtggggagc gatgcgctat ggttataagc tgatttcatt gctctgggcg gtcgtgttga
     1501 tgatcagcat ccacagctat caaaattaca ttcccattta tcctggctat accacgcagc
     1561 tgaccataac ctcctccagt tatctggtat tctcttttat tgtcaattat atggctgtac
     1621 tggcaacccg tcagcgagcg gtagtcagac gcattcagcg gcttgcgtat gtggacccgg
     1681 tggttcatct gccaaatgtt cgcgccctga atcgcgcgtt acgtgatgcc ccctggtctg
     1741 cgctttgtta tttacgcatc cctggcatgg aaatgctggt taagaattat ggcatcatgc
     1801 tgcggattca atacaagcaa aaactttctc actggctgtc accattgctg gaaccgggtg
     1861 aagatgttta tcagctttcg ggtaacgatc tcgcgctgcg gctgaataca gaatcgcacc
     1921 aggagcgcat taccgcactg gatagccatc tcaagcaatt tcgtttcttt tgggatggaa
     1981 tgccgatgca accgcagatt ggcgtcagtt actgctatgt gcgctcgcca gtgaatcata
     2041 tctacctgct gctgggagag ctaaatacag tggccgaact ttctatcgtg accaacgccc
     2101 cggaaaatat gcagcgtcgc ggagcaatgt atttgcaacg cgaattgaaa gataaagtcg
     2161 cgatgatgaa tcggctacag cgggcgctgg aacacaacca ttttttcctg atggcccagc
     2221 cgattaccgg tatgcgtggt gatgtctacc atgaaattct tctgcgcatg aaaggtgaga
     2281 atgatgaact gatcagcccc gatagcttct taccggtcgc gcacgaattt ggtttatcgt
     2341 cgagtatcga catgtgggtc attgagcata cgctgcaatt tatggctgaa aacagagcga
     2401 agatgcccgc tcaccgtttt gcgattaatc tgtcgccaac ctcggtatgt caggctcgtt
     2461 ttcctgttga aatcagtcag ctgctggcta aatatcagat tgaagcgtgg caacttattt
     2521 ttgaagtcac cgaaagtaat gctctgacca atgttaagca ggcgcaaatc accttgcagc
     2581 atcttcagga attaggctgc cagattgcga ttgatgattt cggcaccggc tacgccagct
     2641 atgcgcggct taaaaatgtg aatgccgatc tgcttaaaat tgacggcagt tttatccgca
     2701 atattgtgtc aaatagtctg gattatcaga tagtggcgtc gatttgccac ctggcgcgaa
     2761 tgaagaaaat gcgggtagtg gcagagtacg ttgaaaacga agagatccgc gaggcggtgc
     2821 tctctttggg gatcgattat atgcagggtt atcttattgg taagccgcaa ccgttaattg
     2881 atacgctgaa tgaaatcgaa cccattcgcg aaagtgcctg aataatgcgg gccgacattt
     2941 ctcgtcggcc cgcaaagcat taagcggcga tttctggtgt actttcttct tcaattttca
     3001 accgccagcc agccacgcct tcccagtatt cttgctcctt ttccagatca agcagtacca
     3061 gcgcattctg actaaaccag tcatgcggaa aacgcagtgt ccagtgactg tcatcggtaa
     3121 tcagcgtcaa tgttggcggt gtggtggttg cctgacgttg attattgagt aatacgccaa
     3181 ggcgcaatag ctgaatcagt ggcaggaact gtttcttctt aaacaaggta aagcgcggca
     3241 gatcgtcgag cttaatcgct ttacggtgat agcgcaccag cgtcgccatc atcaactgct
     3301 gttcctgatt aaaacccggc aagtcgctgt tttgcagaat ataagcggag tggcgatgca
     3361 aaccgctgtg gttgatattc aacccgacct catgcagcat ggcggcccat cgcagtagcg
     3421 cttccagttg cggatgcgcc agcttcggtt gctgttcccg ccactgttcg tacatttgca
     3481 tagtggtatc cagcacccgt cgggcctgtt cgctgtcgat gtgatactgg ttggcgaggc
     3541 tgctggcggt gcgactacgc acatcctgat gacggaaacg tccttccatt tcatacagta
     3601 cgccttcgcg taacgccccg tcagaaaggc gcagttcacg gatggctaaa gcatcaaaca
     3661 caccgcataa aatcgccagt ccaggaacga agactgtttt ccgttcttcg gaaagacctg
     3721 gtaagctcag cgatgcgaaa ttacggtgcc gtaaaacttc ttttaccagt ttttccagac
     3781 gttccggggt aattatcccg tctttctcgc ccatttccat taacacttca tgggcggctt
     3841 ttattgtacc ggaagcgccc attgcaacat tccagccctg aattcgaaat tgccaggtta
     3901 aagtttccag tttttgtgct gccgccatgc gagcgcgctg aaaattctct ttattgatga
     3961 ccccgccagg gaaataaagc tgggcaaagc tgacacaacc catccggcgg ctttcaacga
     4021 gaatcggttc gaaattttca ccaatcacca gttccgtaga tccgccgcca atatcaataa
     4081 ccagtttgcg acctttctcc ggttgggtat gttccacgcc cataaaaatc agacgggctt
     4141 cttcattacc ggaaataatt tcaatcgggt agggaatgac cttttccgcg cgtttcagaa
     4201 agtcagtggc gttcagcgcc tgacgcagtg tatgggtgcc aactatacag acgctggcag
     4261 gagaaaaccc ttgcagccgt tcggcaaaca gcgacagaca gtttaaaccg cgcgtcattg
     4321 cctcttcact caacatatta tctggcccca ggccgtccgc cagatgcact cgctgtttca
     4381 ggcggccaat aatctgcatg gcaccatcta ccacacgggc tatgaccatg tgaaaactgt
     4441 ttgaaccaag atcgaccgca gcaaactcct gcggacgagg ggatttatcg tgtattggca
     4501 tagggttatt caggttgttc gagtgatttg atgtagtcgt aaatcgccaa ctgcgcccgt
     4561 actttacggc gattgccgcg gggaacgtag cgattactga gttctttatc gatataacgt
     4621 gctttgaccg tatcgctgaa caatatgtcg atgatgtcca gcacccgctg cttcaggcgc
     4681 ggatcgagca gcggtgtcgc cacttcaata cgataatcaa tattgcgcgt catccagtcg
     4741 gcggaagaaa ggtagacctt tttatcgccg ccattttcaa aaatataaac ccggtcatgt
     4801 tcaaggtaac ggtcaacaat actgatggca cgaatgttgt cgctaatgcc ttccagattg
     4861 gggatcagcg aacacatgcc gcgaaccagc agattaaccg gtacgccgga gctggaggcc
     4921 gcatacagac gatcaaccag gcctttatcg acaaggttat ttagcttcag ggtgataccg
     4981 ctgggcagcc cttgctgcgc gttggcgatt tcgcggtcca ccatttcata caacaggcgg
     5041 cgggagtttt gcggcgacac cattaaataa tcaaatgtca ccggacggta tgggttttca
     5101 ataaagttga acacccgccg tacttcgttg gttatgcgcg catcggcggt cagcaacgaa
     5161 tagtcagtat aaagacgcgc ggttttttcg ttaaagttcc cggtcccgat gtgtgcgtaa
     5221 cgcaccactt caccgttttc tttacgtgaa atcaggaaca gtttggcgtg aattttcagc
     5281 cccggcgcag agaagataac gtgcacgcct gcttcggtca ggcgcttcgc ccagtgaatg
     5341 ttggcttctt cgtcgaaacg cgcctgtaac tcaaccacca cggtgacttt cttaccgttg
     5401 tgtgcggcgt ggatcatcga gtcgatgatg cgtgaatctt tcgccacgcg gtaaatgtta
     5461 attttaatcg ccagtacgct cgggtcgaac gaagcctgac gcagcagttc cagtacatgc
     5521 tcaaaggtat gataaggata atagagcaac acatcgcgtt cgcgaatagc atcaaaacca
     5581 ttgcggaact gggctttatc aaaccaaatg tggcgtaaac gcggcagtgg tttgttcacc
     5641 agattggctt tgccgacatt ggggaaatta ataaagtctt taaaattatg ataacgacca
     5701 ccggggacga tggagtcgta gcgggaaata gtcagttttt cgcgtaacac ttcaaccagc
     5761 gcattgggca tatcgcgctg ataaacaaaa cgcaccggct cagcagttaa acgctgcttg
     5821 agactggaag acatcaactc catcaggctg gcttccatct catgcactaa atcgtattcg
     5881 gcatcgcggg tcatcttcat tgaataggca ttcagcgcgt cataatcaaa gaagcctttg
     5941 aaaatatcat cgaggcagta acgcagaatg ttatccagaa gaatcatcgg cttgcgtcga
     6001 cgtggcgctt ctggcggtaa attcacaaag cgcggcactt tatctgatgg gatctccagc
     6061 agcgcgtaac ggatggtatc gccacggata atttccaccg ccagataggt gtaatcatct
     6121 ttcaggaact gcactaagtc agtgtcagga ttgattaaaa tcggcgtaat gtgctgacgc
     6181 agatactgct taaaataatg acgcagccag ttttgttgat tgacggagag ctggcgttca
     6241 ttaatcagga agatctggtt gcgcgccatc tccagcaaca gctcgttgta gaggccgtcg
     6301 aattcctgat cggctttcag cacccgggac tgaattttgc ccagtaaatg gcgggaatga
     6361 gagttggaac cttgttcttc gctaataatg atgcgtcgct tcagttcagc gaagcggact
     6421 ttatagaact catcaaggtt attggaatag atccccagga aacgcatcct ttcaatcagc
     6481 gggttagatt tgtccgccgc ttcctgaagc acgcgttcat tgaacgataa ccaactgagc
     6541 tctttttcga tgtatagctt ttcctgaccc attaccactt ttactccgtt ttattcacgg
     6601 gacactgcct ggatattatg gcgagcaata ttggcagatg tccaactatg ccagaaaagg
     6661 gggaaaaaaa ctgctggagc ttgcgattta caggtgaatt gaaaaacgcc agcggcagag
     6721 ctggcgcttt aactacgggg cattactcgt cggcagcgta gccctgcggc ggcagacgtt
     6781 gaccatccag ccacgcggcg ttttcgtgca ttttcagacg accatcggca aaccagctaa
     6841 tcaccagtgg ataaatagcg tgttcctggg tttgcacgcg ggcggtgatg tcatcttccg
     6901 tatcaccagc aaataccggg actttcgcct gtaaaataac cgggccaccg tccagttcat
     6961 cggtgacgaa atgcaccgat gtaccgtgct cttcatcgcc attttccagc gcctgacgat
     7021 gggtgtgtaa tccgggatat ttcggcagca gagaagggtg aatgttcagc aaacgcccgg
     7081 catagtggga gacaaacgcc gggctgagaa tgcgcataaa accagccagc acgaccacat
     7141 cgggtgcgta catgtcgatt tcatgaatca actcccggtc ataggcttca cgactgtcaa
     7201 acgcgctggc gatgagcgta tgcgttgcaa tacccgcctg gcgggcgcgt tcaaggccga
     7261 acgcgtcggc cttattgctg aaaactgccc gtacggtgcc tttaattttg ttggttttgc
     7321 aggcgtcaat aattgcctgc aaattacttc cgttgccgga aataagcacc acaatattca
     7381 ttattcgata accacgcgtt gttcggaatc agaggctttg atgataccga ttttccacgc
     7441 gttttcaccg ttggcattga gcagggcgag agctttgtcc acttccggag caggcagggc
     7501 aataatcata ccgacgccgc agttgaaggt gcgatacatt tcatggcgct caacattacc
     7561 tgccgtttgc agccagttga acacttccgg ccactgccag gaagattcat caatcactgc
     7621 ctgggtatta tctggcaata cgcgcggaat gttttcccag aagccgccgc cggtcaggtg
     7681 cgcaatggca tgcacatcga ccttttcaat caatttcagc actgacttca cgtaaatgcg
     7741 ggtcggtgcc agcagatgat cggctaatgg cttaccatca agttcggtgg tttgcggatc
     7801 acaaccgctg acttcaagaa ttttgcgcac cagcgaatag ccattcgagt gcgggccgct
     7861 ggagccgagt gcaatcagca catcgccgtc gctgacttta gatccgtcga tgatttctga
     7921 tttttctacc acaccaacgc agaaaccagc gacatcgtaa tcttcaccgt gatacatccc
     7981 cggcatttcc gccgtttcgc cacccaccag tgaacagccc gattgcagac aaccttccgc
     8041 aatgccgctg atcaccgctg aagcggtatc aacatccagt tttccggttg cgtaatagtc
     8101 gaggaaaaac agcggttccg caccttgcac caccaggtca ttaacgcaca tggcgaccag
     8161 atcaatacca atggtgtcgt gacgttttaa gtccatcgcc agacgcagct tggtacctac
     8221 gccgtcagtg ccggaaacca gcacaggttc acgatatttt tgcggcaatg cacacagcgc
     8281 accgaagccg cccagaccgc ccatcacttc cggacgacgc gttttcttca ctacgccttt
     8341 gattcttcca accagagcat tacccgcgtc aatatcaaca ccggcatctt tgtagctaag
     8401 agaggtttta tcggtcactg cttgggtccc cacgcgttac ttgcggtaga aaaataaaat
     8461 tcggcgcaat tctaacaggg aaagcaaacg tttgcgagac tgctttacac aacctttttg
     8521 cacgtctttt ccccaggcgc gcggcgaaag aatacttgta ctagggcaaa ggatcgtttt
     8581 cggatggaat aatcttcttc cataaccatt tgaatataaa ataactttat ctcaaaccgt
     8641 tatcattttg actaaagtca acgaaaataa tattgccgcc ttgaagaaag gaggtataat
     8701 ccgtcgattt tttttgtggc tgcccctcaa aggagaaaga gtatgaagat cgtggaagtc
     8761 aaacacccac tcgtcaaaca caagctggga ctgatgcgtg agcaagatat cagcaccaag
     8821 cgctttcgcg aactcgcttc cgaagtgggt agcctgctga cttacgaagc gaccgccgac
     8881 ctcgaaacgg aaaaagtcac tatcgaaggc tggaacggcc cggtagaaat cgaccagatc
     8941 aaaggtaaga aaattaccgt tgtgccaatt ctgcgtgcgg gtcttgggat gatggacggt
     9001 gtgctggaaa acgttccgag tgcgcgcatc agtgtcgtcg gtatgtaccg taatgaagaa
     9061 acgctggagc cggtaccgta cttccagaaa ctggtttcta acatcgatga gcgtatggcg
     9121 ctgatcgttg acccaatgct ggcaaccggt ggttccgtta tcgcgaccat cgacctgctg
     9181 aaaaaagcgg gctgcagcag catcaaagtt ctggtgctgg tcgctgcgcc agaaggtatc
     9241 gctgcgctgg aaaaagcgca cccggacgtc gaactgtata ctgcatcgat tgaccaggga
     9301 ctgaacgagc acggatacat tattccgggc ctcggcgatg ccggtgacaa aatctttggt
     9361 acgaaataaa gaataaaaat aattaaagcc gactttaaga gtcggctttt ttttgagtaa
     9421 agcgcctata acacataata cagaggataa tactatgacg cgccgtgcta tcggggtgag
     9481 tgaaagaccg ccacttttac agacaatccc gcttagtttg caacatttgt tcgccatgtt
     9541 tggtgcaacc gtcctggtgc ccgtcttatt tcatattaac ccggcgactg tactgttatt
     9601 taacggtata ggaacactgc tgtatctctt catctgtaaa gggaaaattc cggcttatct
     9661 tggttccagc tttgccttta tttcaccggt attgttactg ttgccgttgg ggtatgaagt
     9721 tgcgctgggc ggctttatta tgtgcggcgt gctgttctgc ctggtttctt ttatcgtgaa
     9781 gaaagcgggg accggctggc tggacgtgct gtttccacct gcggcaatgg gcgcaatcgt
     9841 tgccgtcatc ggtctggagc tggcgggcgt agctgccggt atggcgggtt tactcccggc
     9901 tgaagggcaa acgccagact ccaaaaccat catcatctct attaccaccc tggcggtcac
     9961 ggttttaggt tccgtgctgt ttcgtggttt cctggcaatt atcccgattt taattggcgt
    10021 gctggtgggg tacgcgctct ctttcgcaat gggaattgtc gataccacgc cgattattaa
    10081 tgctcactgg tttgcgctgc cgaccctcta tacgccgcgc ttcgagtggt ttgccattct
    10141 gactattcta ccagcggcgt tagtggttat tgccgaacac gtagggcacc tggtcgtaac
    10201 ggctaatatc gtcaaaaaag atctgctgcg cgatccaggt ctgcaccgtt cgatgtttgc
    10261 taacggcttg tcgaccgtga tctccggctt ctttggctcc acgccaaaca ccacttacgg
    10321 agaaaacatt ggcgtgatgg cgatcacccg tgtttacagt acctgggtta tcggcggtgc
    10381 ggcgattttc gctatcctgc tttcctgcgt cggtaaactg gctgcagcta tccagatgat
    10441 tccattgccg gtgatgggcg gcgtttcgct gctgctttac ggtgtcatcg gtgcttccgg
    10501 tattcgtgtg ttgatcgaat cgaaagtgga ttacaacaaa gcacagaacc tgatcctgac
    10561 ttccgttatt ttgatcatcg gcgtcagtgg cgcgaaggta aacattggcg cggcggagct
    10621 gaaaggtatg gcgctggcga ccatcgtcgg tatcggctta agtttgatct tcaaactgat
    10681 tagtatgctg cgccctgaag aagtggtgct ggacgcagaa gacgcggata taacagacaa
    10741 ataatgtacc gaccgggcag tgttgctgcc cggttcaaac atcatgggat tctgtggtaa
    10801 acttctcgcg attttgtgaa atcctggttg aggtatctct gaacacaccg gcacagctct
    10861 ctttgccact ttatcttcct gacgacgaaa cctttgcaag tttctggccg ggggataact
    10921 cctctttact ggccgcgctg caaaacgtgc tgcgtcagga acatagcggt tacatctatc
    10981 tctgggcacg cgaaggcgcg gggcgcagcc atctgctgca cgcggcttgc gcggaattgt
    11041 cgcagcgtgg cgatgcggtg ggctatgtcc cgctggataa acgcacctgg tttgttccgg
    11101 aagtgctcga cggtatggag catttgtcgc tggtctgtat cgacaacatt gagtgtattg
    11161 caggcgatga gttgtgggag atggcgattt tcgatctcta caatcgaatt ctggaatcgg
    11221 gcaaaacacg gttgttaatc accggcgatc gtccaccgcg gcagttgaat cttggattac
    11281 cggatctcgc gtcgcgtctc gactgggggc agatctacaa attgcagcca ctttctgatg
    11341 aagataagtt gcaggcgcta cagttacgcg cgcgtttgcg tggttttgaa ctgccggaag
    11401 atgtggggcg tttcttgctg aagcggctcg acagagagat gcgcacgtta tttatgacgt
    11461 tggatcagtt ggatcgtgcg tcgattaccg cgcaacgtaa gctgaccatt ccgtttgtga
    11521 aagaaattct gaaactgtag acaagacgtt tattgtcgga tatgcaccca tagtattcgt
    11581 cggccggata agacgcggca agcgtcgcat ccggcaatgt gcttcaacta gcgatggagc
    11641 acttcttaac ccacgatttc cagtacctgc tccggtggac gaccaatccg cgctttgcca
    11701 ttcgcgacca caatcgggcg ctccattagc ttcggattat ccaccattgc ctgaatcagc
    11761 gcctcttcgc taagcgaact gtcagccagg ttcagttctt tataaagatc ctctttctgg
    11821 cgcatcaatt cacgagcgct attcatcccc agcattttca gcaaatcacg cagcgttgcc
    11881 gcatcggcgg gtgtctcaag gtagagcacc acttctggct ccacgccgtt ttctttcagc
    11941 aggttcagcg tttcccggct ctttgaacag cgtgggttat ggtagatttt tacctgtttg
    12001 gtcattgttc ttcctttaat gcgaattaca tcttggtata aggcttaaag cgttcctgca
    12061 gctggcgcaa ctggtcgatg cgcgcatcgt aacgcgcttg ttgcaggctg cctaatttca
    12121 cctgcgaact ggcgctactc aacagcgaaa tggcctgatc gagtcgtccg gcgagcgcat
    12181 aaccttctgc tcgcgcagcc agctcctgat cgcggttatt tagcgcggct tccgcctgtg
    12241 ccagcaaatc ccagccgttg ctgtcatctt tattattaaa ggtgtagcga ttcagaatat
    12301 tggccgcttc ttgtggttga ccgccttgca gataagcgtt cgccaggttg agctgcaaca
    12361 ccggattggt gcgtaaatcg cgggcatttt tcagacgatt gatcgcctca ttggctttgt
    12421 tttgcccaag atcgatatca gtagccagat cgagatacca tgcgttgcca ggttctgccg
    12481 ccagtaacgg ttgcagcgtt ttacgcgcct cgtcgtattt attggcttcc atcgcctgta
    12541 aagcacgacc atattgcgcc gctcgttgct gacgaacgtt tcctttcgcc cattcatcca
    12601 gcaaatcact ggtgagctgg ttacgtccgg aattatacat ccccagtgtg cgcgctttcg
    12661 ccagatagaa atcttccgac gactgcacca ccatcgggcg catctgatta gcacggttgc
    12721 gggcatctgc cagacgactt tccggcaacg ggtgagtcaa taaaatttcc ggcggacgcg
    12781 aggagtaacg cgcctgatcg agtaattttt cgaggaaggt cggcatcgcc tgcggatcga
    12841 atcctgagcg ttgcagcacc tgaataccaa tgcggtccgc ttcctgttca ttttgctggg
    12901 tgaagctgat catcccctgg cgcgttcccg ccagtgtacc ggtcagcgca gccatccccg
    12961 cctgcggact ggccatcgcc agtaaaatag aacctaacgc gccgacccag gtcagcggcg
    13021 cgctgcgctg ctgatcttcc atcgctcgcg ccaggtgacg ttgggtgacg tgggagattt
    13081 cgtgcgccat aactgaagcc agttgacttt cgttatcgga ataacggaac agggcagagt
    13141 gcagcaccac gttgccgcca aagaaagcaa aggcgttaat ttcatcgttg ttgatcagaa
    13201 agaaatgaaa cggtgtctta accgaattgg catgcgaaac cagacgcatt cctagcgaat
    13261 taatgtattg cgttaacagc ggatcattaa ttaacggcgc gctgccgcgt agctggcgga
    13321 cataatagtc gcccatctgc atttcctgac caatggaaag tgtgcttcct gcggaggttc
    13381 ccatatccgg caaggtgtct gcgctgtcgg caaacgccgg ggctacctga ccaatggtca
    13441 tagcagcaat gagggttgca accaggtttt ttttcaactg cctgaacata acctctatcc
    13501 tgtatttctg gagatagtca tttgaccgat actgtgaaat atcgttccca ccgcgccgcc
    13561 tttgagtgta acggcgaaga ttagcccact caaatgactt ttcagtttcc tgaatccgtc
    13621 ataaaaagcg aagtcttttt tgtggcattt cgatacaatt gcggatcgca atcccgctct
    13681 tgaggttcag ggaaggtctg tatgctcgaa atgttgatgc aatggtatcg ccgccgtttt
    13741 agcgacccgg aagcgattgc cttgctggtt attttagttg ccggatttgg cattatcttt
    13801 ttctttagtg gcctgcttgc tccgctgttg gtggctattg tgctggccta tttgctggaa
    13861 tggccaaccg tgcgcctgca atctattggc tgctcccgcc gctgggcgac gtcgattgta
    13921 ttggtggttt tcgtcggtat attgctactg atggcgttcg tggtactgcc tatcgcctgg
    13981 cagcagggca tctacttaat ccgcgatatg ccggggatgc tcaataagct ttctgacttt
    14041 gccgccacgt tgccgcgccg ctatccggcg ttaatggatg cgggcattat tgatgcaatg
    14101 gccgaaaata tgcgcagtcg gatgctgacc atgggcgatt cggtggtgaa aatttccctc
    14161 gcctcgctgg tcggtttgct gaccattgcc gtctatctgg tgctggtacc attgatggtc
    14221 ttcttcctgc tgaaagacaa agagcagatg ctgaacgccg ttcgccgggt gctgccgcgt
    14281 aaccgtggac tggcaggaca ggtgtggaag gagatgaatc aacaaatcac caactatatc
    14341 cgcggcaaag tgctggagat gatcgtggtg gggattgcca cctggctggg attcttactc
    14401 ttcgggctga actattcgct gctgctggcg gtgctggtcg gcttctcggt acttattccg
    14461 tacattggtg catttgtggt gaccattccg gtggttggcg tggcgctatt ccagtttggt
    14521 gctggcacgg aattctggag ctgcttcgcg gtgtatctga tcattcaggc gctggacggc
    14581 aacctgttag tacctgtgtt gttctccgaa gcggttaacc tgcatccgct ggtcattatt
    14641 ttatcagtag tgatcttcgg tggtttgtgg ggattctggg gcgtattctt cgccattcca
    14701 ttggcgacgc tgatcaaagc cgtgattcac gcctggcccg atgggcaaat cgcgcaagac
    14761 tgaccttcag cgaaaaaatt tgaaacgagt gttgcagggc agtgagaaga tgtgagatgt
    14821 gctggccgca agcgcagcca gcacggaatg gagcaaagta atcaggcgtg ttctttcagc
    14881 cagttcagca caacgtcgtg gtgattgctg gttttgaaat cgtcaaagac atgttcgatt
    14941 ttgccatcag cgtcaatcag gaagctgatg cgatgaatac catcgtaggt tttgcccatg
    15001 aaggactttt caccccagac gccgaattgt tcgcacacct ggtggtcctc atcagacagg
    15061 agcgtaaagt taagcagctc tttttccgca aaacgggaga gtttttcggg tttatcggtg
    15121 ctgataccca gcacatcaac gcccgctttt ttcaactcat ccatgttatc gcgtaagccg
    15181 caggcctgta cggtacagcc gggggtcatg gctttcgggt agaaataaac cagaacacgc
    15241 tgtccctgga agtcggtcaa attaacttgt tctccgtctt gatccggcaa gctaaatttc
    15301 ggtgcgatat caccggcttt cagtggattc attacttaac tccatcctgt tcatcatgtt
    15361 gggaataatt gacgacgtta atactgcctt gtgcattgag ttctgtacat agggctttga
    15421 atgcttgctc aatatttgct gcgtccgcag atgcggggct gtgggcggtt atctgaatat
    15481 gcaactgcgc agcccgttca ttttcagcag gttgcgtgcg cgacaccagc tccgcaatgt
    15541 tcatatgatg cgcgtcgaaa agtgctgtga agcgttcaat taaatgcggg gagtctgcca
    15601 catcgacctg aacccagaca gatgctggca ttggcggacg cggacgcgcc gtcgtgcgct
    15661 tcatcacgat taaaagatcc agctcggcac ctttcaacgg taacgttgat tcaatcagag
    15721 taatggcatt ccatgaaccg gaaagcagca tgataaacgt gaactcttct cccagcatcg
    15781 ccaggcgact gtcttcaata ttacagccgc aactactgac atgacgggtg atggtgttca
    15841 caattccagg gcgatcggca cccaacgcag tgatcaccag ataatgttgc gatgacagtg
    15901 tcaaactggt tattccttca aggggtgagt tgttcttaag gaaagcataa aaaaaacatg
    15961 catacaacaa tcagaacggt tctgtctgct tgcttttaat gccataccaa acgtaccatt
    16021 gagacacttg tttgcacaga ggatggccca tgttcacggg aagtattgtc gcgattgtta
    16081 ctccgatgga tgaaaaaggt aatgtctgtc gggctagcat gaaaaaactg attgattatc
    16141 atgtcgccag cggtacttcg gcgatcgttt ctgttggcac cactggcgag tccgctacct
    16201 taaatcatga cgaacatgct gatgtggtga tgatgacgct ggagctggct gacgggcgca
    16261 ttccggtgat tgccgggacc ggtgctaacg ctactgcgga agccattagc ctgacgcagc
    16321 gcttcaatga cagcggtatc gtcggctgcc tgacggtaac cccttactac aatcgtccgt
    16381 cgcaagaagg tttgtatcag catttcaaag ccatcgctga acatactgac ctgccgcaaa
    16441 ttctgtataa tgtgccgtcc cgtactggct gcgatctgct cccggaaacg gtgggccgtc
    16501 tggcgaaagt aaaaaatatt atcggaatca aagaggcaac agggaactta acgcgtgtaa
    16561 accagatcaa agagctggtt tcagatgatt ttgttctgct gagcggcgat gatgcgagcg
    16621 cgctggactt catgcaatta ggcggtcatg gggttatttc cgttacggct aacgtcgcag
    16681 cgcgtgatat ggcccagatg tgcaaactgg cagcagaagg gcattttgcc gaggcacgcg
    16741 ttattaatca gcgtctgatg ccattacaca acaaactatt tgtcgaaccc aatccaatcc
    16801 cggtgaaatg ggcatgtaag gaactgggtc ttgtggcgac cgatacgctg cgcctgccaa
    16861 tgacaccaat caccgacagt ggtcgtgaga cggtcagagc ggcgcttaag catgccggtt
    16921 tgctgtaaag tttagggaga tttgatggct tactctgttc aaaagtcgcg cctggcaaag
    16981 gttgcgggtg tttcgcttgt tttattactc gctgcctgta gttctgactc acgctataag
    17041 cgtcaggtca gtggtgatga agcctacctg gaagcggcac cgcttgcgga gcttcatgcc
    17101 ccggctggaa tgattttgcc ggtgacctcc ggcgattatg caatcccggt gaccaacggt
    17161 agtggtgctg tcggtaaggc gctggacatt cgtccaccag cccagccgtt ggcactggtt
    17221 tctggcgcgc gtacccagtt cacgggcgat accgcttcat tgctggtgga aaatggtcgt
    17281 ggcaatactc tgtggccgca ggtggttagc gtgctgcagg cgaaaaacta caccatcacc
    17341 caacgtgatg atgctggtca gacactgacc accgattggg tacaatggaa ccgtctggac
    17401 gaagacgagc agtatcgtgg tcgttatcaa atctctgtta agccgcaggg ttatcagcag
    17461 gcggttacgg ttaaactgct gaacctggaa caggcgggca aaccggttgc agacgcggct
    17521 tccatgcagc gttacagcac ggagatgatg aacgttattt ccgccggtct ggataaatct
    17581 gccactgacg ccgcgaacgc tgcgcaaaat cgtgcctcca ccactatgga cgtacaaagt
    17641 gcagctgatg acaccggttt accaatgctg gtcgtacgcg ggccgttcaa tgtggtttgg
    17701 caacgtctgc cagctgcgct ggaaaaagtg ggcatgaaag tgaccgacag cacccgttcg
    17761 cagggcaaca tggccgtaac ttacaagccg ctgtctgaca gcgaatggca cgaactgggc
    17821 gcgagcgatc caggtctggc atccggtgac tataaactgc aggttggcga tttagataac
    17881 cgcagcagcc tacagttcat cgatccgaaa ggtcatactc tgactcagag tcagaacgac
    17941 gcgctggtag ctgtcttcca ggctgcgttt agcaagtaaa aatacagggc tggagtcatc
    18001 cggccctttt ttctgatatg atacgcaaac gtgtgcgtcg gcagaaaaac gcgattttag
    18061 cggtaattcg cacgaaattt gtttgtcgga cgtaggtcgg ataaggcgtt cacgccgcat
    18121 ccgacaaaac atccggcaca ccagacagca atagatttta aaacgttaat tcacacccag
    18181 gagtgataaa gatgcaaaag caagctgagt tgtatcgtgg taaagcgaaa accgtataca
    18241 gcacggaaaa cccggacctg ttggtgctcg aattccgcaa tgatacgtca gcaggggatg
    18301 gcgcgcgcat tgagcagttt gatcgcaaag gtatggtgaa caacaagttc aactacttca
    18361 ttatgagcaa actggctgaa gcgggtatcc cgactcaaat ggagcgtctg ctctccgata
    18421 ccgaatgtct ggtgaaaaag ctggatatgg tgccggttga gtgtgtcgtg cgtaaccgtg
    18481 ctgctggctc tctggtgaaa cgtcttggaa tcgaagaagg tattgagctg aacccgccac
    18541 tgttcgatct gttcctgaaa aacgacgcca tgcacgatcc gatggtcaac gaatcttact
    18601 gcgaaacctt tggctgggta agcaaagaga acctggcacg catgaaagag ctgacctaca
    18661 aagcgaacga cgtgctgaaa aaactgttcg atgatgctgg tctcattctg gtcgacttca
    18721 agctggagtt tggtctgtac aaaggtgaag tggtactggg cgatgagttc tccccggacg
    18781 gcagccgctt gtgggacaaa gaaacgctgg agaaaatgga caaagaccgt ttccgccaga
    18841 gcctcggtgg cctgatcgag gcctatgaag ccgtcgcccg ccgcctgggt gtacagctgg
    18901 actgattttt ctgttcatca tcttgccgtg cagctggcac ggcaagacaa ccgctctcat
    18961 aagatgagta ttgaacgctc attcctccgc catttcatcc cgctttcctg tggtaatctt
    19021 cctctgaacc acctacgatc atttctataa tgaatatatt gttgaggtat ctatgcgttg
    19081 gcaagggcga cgtgaaagtg acaatgttga agacaggcgc aacagctctg gtggtccgtc
    19141 aatgggcggt ccaggttttc gcctgccaag cggtaaaggc gggctgattt tactgatagt
    19201 cgtgctggtt gcaggctact atggcgttga tttaaccggg ttgatgaccg gacagccggt
    19261 ttcccaacag caatcaacac ggtcaattag tcctaatgaa gacgaagccg caaaattcac
    19321 ttcggtgatt ctggcaacca cggaagacac ctggggacag caattcgaga agatgggtaa
    19381 aacctatcag caaccgaagc tggtcatgta ccgtggaatg acgcgcaccg gctgcggggc
    19441 gggccagtcc ataatgggac cgttctattg cccggcggat ggcacggttt atatcgatct
    19501 ctccttctat gatgacatga aagacaaact tggcgcggat ggcgattttg cccaggggta
    19561 cgttatcgcc catgaagtcg gtcatcatgt gcagaaactg ttaggcatcg agccgaaagt
    19621 tcgtcaactg caacaaaacg cgacgcaggc ggaagtgaac cgcttatctg tgcgtatgga
    19681 actccaggcc gactgttttg ccggtgtctg ggggcatagt atgcagcagc aaggtgttct
    19741 ggaaaccggc gatctggaag aggcgctgaa cgcggcgcag gccatcggcg atgaccgttt
    19801 acaacagcaa agtcaggggc gagtagtacc agacagtttc actcatggca cttctcagca
    19861 acgctacagc tggtttaaac gtggtttcga cagcggcgat ccggcacaat gcaatacttt
    19921 tggtaaaagc atttaacttc cggggcaggg atggctgaac tgactgcgct tcacacatta
    19981 acagcgcaaa tgaaacgtga agggatccgc cgcttgctgg tgttgagcgg ggaagagggt
    20041 tggtgttttg atcatgcgct taagttgcgt gatgccttac ctggcgactg gctgtggatt
    20101 tcgccgcagc cagatgctga aaaccactgt tctccctcgg cgctacaaac tttacttggg
    20161 cgcgagttcc ggcatgcggt attcgacgcc cgccacggct ttgatgccgc tgcctttgcg
    20221 gcacttagcg gaacgttgaa agcgggaagc tggctggttt tgttactccc tgtatgggaa
    20281 gagtgggaaa accaacctga tgccgactcg ctgcgatgga gtgattgccc tgaccctatt
    20341 gcgacgccgc attttgtcca gcatctcaaa cgcgtactta cggcgaataa cgacgctatc
    20401 ctctggcggc agaaccagcc gttcacgttg gcgcatttta ctccccgtac tgactggcac
    20461 cccgcgaccg gcgcaccaca gccagaacaa cagcaactct tacagcagct actgaccatg
    20521 ccattgggcg tggcggtggt aacggctgcg cgtgggcgcg gtaaatcggc gctggcaggg
    20581 caactcattt ctcgtattgc gggtagtgcg attgtcaccg cgcccgcaaa agcggcaacg
    20641 tatgtactgg cacaatttgc gggcgagaag tttcgcttta ttgcgccgga tgccttgtta
    20701 gccagcgatg agcaagccga ctggctggtg gtcgatgaag ccgcagccat acctgcgccg
    20761 ttgttgcatc aactggtatc gcgttttcct cgaacgttgt taaccactac ggtgcagggc
    20821 tacgaaggca cgggacgtgg ttttttgctg aaattttgcg ctcgctttcc gcatttacac
    20881 cgttttgaac tgcaacagcc gatccgctgg gcacagggat gcccgctgga aaaaatggtt
    20941 agtgaggcac tggtttttga cgatgaaaac ttcacccaca caccacaagg caatatcgtc
    21001 atttccgcat ttgaacagac gttatggcga agcgagccac aaacgccgtt aaaggtttat
    21061 caattattgt ctggtgcgca ctaccggact tcgccgctgg atttacgccg catgatggat
    21121 gcaccagggc aacatttttt acaggcggct ggcgaaaacg cgattgccgg ggcgctgtgg
    21181 ctggtggatg agggcggatt atctcaagaa ctcagtcagg cggtatgggc aggttttcgt
    21241 cgcccgcggg gtaatctggt ggcccagtcg ctggcggcgc acggcagcaa tccactggcg
    21301 gcgacattgc gcggaaggcg ggttagccgg atagcagttc atccggctcg tcagcgggaa
    21361 ggcacaggtc agcaactgat tgctggtgct ttgcaatata cgcatgacct cgactatctt
    21421 tcggtgagtt ttggttacac cggggaatta tggcgtttct ggcaacgctg cggttttgtg
    21481 ctggtgcgaa tgggtaatca tcgtgaagcc agcagcggtt gctatacggc gatggcgctg
    21541 ttaccgatga gtgatgcggg taaacagctg gctgaacgtg agcattaccg tttacgtcgc
    21601 gatgcgcaag ctctcgcaaa gtggaatggc gaaacgctcc ctgttgatcc actaaacgat
    21661 atcgtgcttt ctgccgacga ctggcttgaa ctggccggtt ttgctttcgc tcatcgtccg
    21721 ctattaacgt cgttaggttg cttaatgcgt ctgttacaaa ccagcgaaat ggcattaccg
    21781 gcgctgcgtg ggcgtttaca gaaaaacgcc agcgacgcgc agttatgtac cacacttaaa
    21841 ctttcaggcc gtaagctgtt actggtccgt cagcgggaag aggccgcgca ggcgctatac
    21901 gcacttgatg atgttcgcac tgagcgtttg cgcgatcgca taacgcaatg gcaatttttt
    21961 cactgactcc ttcaattttc tcccatggtc attgtgagtc agcggcgtaa aattaatgac
    22021 atcagttaag gagttcgcca tgaaacatga ccattttgtt gttcaaagcc cggataaacc
    22081 tgcgcaacag ttgttactgc ttttccacgg tgtcggggat aacccggtgg caatggggga
    22141 aataggtagc tggtttgcgc cgctatttcc tgatgcgctg gtggtgagtg tcggaggcgc
    22201 ggagccgagc ggtaatccgg cggggcgtca gtggttttcg gtgcagggta ttacggaaga
    22261 taatcgccag gcgcgtgtgg atgccatcat gccgacgttt attgagacgg tgcgctactg
    22321 gcagaaacag agcggagtag gggctaccgc cacggcgtta atcggtttct ctcaaggcgc
    22381 gattatggcg ctggagagca ttaaagccga accgggcctt gcctcacgcg tcattgcctt
    22441 taacggacgc tatgcgagcc tgccggaaac tgcgtctaca gccacgacaa ttcacctgat
    22501 tcatggcggt gaagatccgg ttatcgatct ggcgcatgcg gttgcggcgc aggaagcgtt
    22561 aatcagtgcc ggtggtgacg tgacgctgga tatcgtggag gatttaggcc atgcgatcga
    22621 caatcgcagt atgcaatttg ctctggatca cttgcgttat accattccga aacattattt
    22681 cgacgaagcg ctaagcggcg gtaagcctgg cgatgatgat gtgattgaaa tgatgtaata
    22741 atagccgggt aggtctgata agactgtatc aggcatcatg gccggatgcg gcgttaacgc
    22801 cttatccggc cacttagctt acttcttcgg ttgatccttt ttcggccagt cgtcgtcatc
    22861 gtcccactta tcgttgaaat cacgatgcgg gggaagctcc ggcttgttgg cgagaaattt
    22921 cttatggttg acgcgcttga gatctttaat cacgttcagc aggacgccta ccaaaaagac
    22981 aatcaccaga atccaccaat atttagccag ccagtccatg cttatttcct cttgcagaac
    23041 cactcatcag gcgacgagct gttccatgat acgttgatac atacgggcaa gtagctgcag
    23101 gtcggcagcg ttcacacatt cattaatttt atgaatagtg gcattgaccg gcccgagttc
    23161 caccacctgc gcccccatgc gggcaataaa gcgcccgtcg gacgttccgc ctgtggttag
    23221 tagctgcggt ttaatttcat tatagtgctc aaccgcgtta acgaccgcat ccaccagttt
    23281 accgcgcgcg gtcaaaaatg gctgcccgga aagccaccaa tccaccgtat agcgcagttg
    23341 atgtttttca agcagggcaa gtacctgcgc tttgatcatc tcatcagtca gttcggtgct
    23401 gaagcggaag ttaaactgca caaacagttc acccggaata acgttgttac tgcccgttcc
    23461 cgcctgaata ttggcaatct gcatactggt cgccgggaag aattcattgc cctgatccca
    23521 ctcaatagcc actaattcat taaggaaagg tgctgcgcga tgtaccggat tgtcagccag
    23581 atgtgggtag gcaacatgcc cctgcacgcc atgaatggtg aggttgcagg ttaacgatcc
    23641 gcgacgacca tttttcacca catcaccaac cacttcgata ctcgacggtt cgccaaccag
    23701 acagtaatcg agacgctcat tacgcgccat taacgcttcg acgactttta ccgtaccgtt
    23761 gtgggcacta gcttcttcat cagaggtgat cagaaatgcc agtcgccccg tatggttggg
    23821 atgttgtgcg acaaaacgtt ctgccgctac caccatcgcc gccagcgagc ctttcatatc
    23881 tgccgcaccg cgcccgaata acatgccgtc acgaatggtg ggttcaaacg gcggattgat
    23941 ccaacggtcg gcgtcgccag gcggcaccac gtcggtatgc ccggcaaagg ctaacgtttc
    24001 accctgccca cgccatgccc aaaaattctg cgtatcggca aagtccatgc gttcaacggt
    24061 aaaaccgatc gcctgcaaac gttcaatcaa caaagcctgg catcctgcgt catcaggact
    24121 cagggaaggg cggcgaataa gctgttgtgt cagctcaata accgggcacg acatagacta
    24181 cacctcatgg aaaaattgct gataactgga ttcactgaaa cccagcagca taggcttacc
    24241 aggcgcgcag agcaatggac gtttgataat tgcaggcatt tcagtcatta atgtcgccgc
    24301 agaggccgca tcggtgattt tattgcgggt ggtttcgtcc agtttacgcc aggttgtacc
    24361 acgggtgttg agtaacgctt cccagcctaa ttcgttgata aaaccgttca ataattcgct
    24421 gtccaatcca tcgacgcggt aatcatgaaa gcgatagtcg atgttattgg cttccagcca
    24481 acggcgagcc tttttaatgg tgtcacaatt tttgatgccg taaagtgtaa ccataatgaa
    24541 tcctttttcg atttatgaga ataaatactc atttaagggt gaaaacattt tgcctgcaaa
    24601 t
//
LOCUS       DAZKGF010000069        24599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZKGF010000069 DAZKGF010000000
VERSION     DAZKGF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.069"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(37..402)
                     /gene="yhaH"
                     /locus_tag="ACHOQV_002562"
     CDS             complement(37..402)
                     /gene="yhaH"
                     /locus_tag="ACHOQV_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9630922.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(693..1679)
                     /gene="yqjG"
                     /locus_tag="ACHOQV_002563"
     CDS             complement(693..1679)
                     /gene="yqjG"
                     /locus_tag="ACHOQV_002563"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFP9630923.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFNHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1749..2141)
                     /gene="yqjF"
                     /locus_tag="ACHOQV_002564"
     CDS             complement(1749..2141)
                     /gene="yqjF"
                     /locus_tag="ACHOQV_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP9630924.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2328..2627)
                     /gene="yqjK"
                     /locus_tag="ACHOQV_002565"
     CDS             complement(2328..2627)
                     /gene="yqjK"
                     /locus_tag="ACHOQV_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFP9630925.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2617..3021)
                     /gene="yqjE"
                     /locus_tag="ACHOQV_002566"
     CDS             complement(2617..3021)
                     /gene="yqjE"
                     /locus_tag="ACHOQV_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9630926.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3024..3329)
                     /gene="yqjD"
                     /locus_tag="ACHOQV_002567"
     CDS             complement(3024..3329)
                     /gene="yqjD"
                     /locus_tag="ACHOQV_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFP9630927.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3367..3735)
                     /gene="yqjC"
                     /locus_tag="ACHOQV_002568"
     CDS             complement(3367..3735)
                     /gene="yqjC"
                     /locus_tag="ACHOQV_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFP9630928.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3882..4265)
                     /gene="mzrA"
                     /locus_tag="ACHOQV_002569"
     CDS             complement(3882..4265)
                     /gene="mzrA"
                     /locus_tag="ACHOQV_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFP9630929.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4269..4931)
                     /gene="yqjA"
                     /locus_tag="ACHOQV_002570"
     CDS             complement(4269..4931)
                     /gene="yqjA"
                     /locus_tag="ACHOQV_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFP9630930.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(4953..5036)
                     /locus_tag="ACHOQV_002571"
     CDS             complement(4953..5036)
                     /locus_tag="ACHOQV_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630931.1"
                     /translation="MLMYQTRRTYQDSNNIAVVHHLKPAWR"
     gene            complement(5276..6052)
                     /gene="exuR"
                     /locus_tag="ACHOQV_002572"
     CDS             complement(5276..6052)
                     /gene="exuR"
                     /locus_tag="ACHOQV_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFP9630932.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQATDNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6182..7480)
                     /gene="exuT"
                     /locus_tag="ACHOQV_002573"
     CDS             complement(6182..7480)
                     /gene="exuT"
                     /locus_tag="ACHOQV_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFP9630933.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            7963..9375
                     /gene="uxaC"
                     /locus_tag="ACHOQV_002574"
     CDS             7963..9375
                     /gene="uxaC"
                     /locus_tag="ACHOQV_002574"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9630934.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9390..10877
                     /gene="uxaA"
                     /locus_tag="ACHOQV_002575"
     CDS             9390..10877
                     /gene="uxaA"
                     /locus_tag="ACHOQV_002575"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFP9630935.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10960..11511
                     /gene="ygjV"
                     /locus_tag="ACHOQV_002576"
     CDS             10960..11511
                     /gene="ygjV"
                     /locus_tag="ACHOQV_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFP9630936.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11516..12760)
                     /gene="sstT"
                     /locus_tag="ACHOQV_002577"
     CDS             complement(11516..12760)
                     /gene="sstT"
                     /locus_tag="ACHOQV_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP9630937.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLINDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12965..13267)
                     /locus_tag="ACHOQV_002578"
     CDS             complement(12965..13267)
                     /locus_tag="ACHOQV_002578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9630938.1"
                     /translation="MASKSPKRSLEAGATRMDARRGRILQGRSLAPDPVAARDKLREP
                     RSGDFVRQSLGVQGAAAIGRPLRAPCASGFIWHSTLKERNRSHIPNSFAHMLSNQP"
     gene            complement(13376..14341)
                     /gene="alx"
                     /locus_tag="ACHOQV_002579"
     CDS             complement(13376..14341)
                     /gene="alx"
                     /locus_tag="ACHOQV_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFP9630939.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(14624..15610)
                     /gene="ygjR"
                     /locus_tag="ACHOQV_002580"
     CDS             complement(14624..15610)
                     /gene="ygjR"
                     /locus_tag="ACHOQV_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9630940.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKNVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFAELVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15689..16372)
                     /gene="ygjQ"
                     /locus_tag="ACHOQV_002581"
     CDS             complement(15689..16372)
                     /gene="ygjQ"
                     /locus_tag="ACHOQV_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFP9630941.1"
                     /translation="MQARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNTSKQL
                     TWSDVSAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVGAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAQK"
     gene            complement(16449..16952)
                     /gene="ygjP"
                     /locus_tag="ACHOQV_002582"
     CDS             complement(16449..16952)
                     /gene="ygjP"
                     /locus_tag="ACHOQV_002582"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP9630942.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            17037..18173
                     /gene="rlmG"
                     /locus_tag="ACHOQV_002583"
     CDS             17037..18173
                     /gene="rlmG"
                     /locus_tag="ACHOQV_002583"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFP9630943.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18364..18447
                     /locus_tag="ACHOQV_002584"
                     /pseudo
     CDS             18364..18447
                     /locus_tag="ACHOQV_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            18444..18860
                     /gene="higA"
                     /locus_tag="ACHOQV_002585"
     CDS             18444..18860
                     /gene="higA"
                     /locus_tag="ACHOQV_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFP9630944.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18905..20923)
                     /gene="fadH"
                     /locus_tag="ACHOQV_002586"
     CDS             complement(18905..20923)
                     /gene="fadH"
                     /locus_tag="ACHOQV_002586"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFP9630945.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVGLAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KSHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMFLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQQLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(21094..23445)
                     /gene="ygjK"
                     /locus_tag="ACHOQV_002587"
     CDS             complement(21094..23445)
                     /gene="ygjK"
                     /locus_tag="ACHOQV_002587"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9630946.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSATLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNKRNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKAETLSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(23462..24532)
                     /gene="ygjJ"
                     /locus_tag="ACHOQV_002588"
     CDS             complement(23462..24532)
                     /gene="ygjJ"
                     /locus_tag="ACHOQV_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFP9630947.1"
                     /translation="MKLITAPCRALLALPFCYAFSVTADEARPAEHDDTKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVSGSES
                     GMMVSAMYEALKGIYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
BASE COUNT         5999 a         6557 c         6319 g         5724 t
ORIGIN      
        1 gccggatgag tcagacatcc ggcgagtaga tttgttttaa ggttccagtt ttggatcagg
       61 tccaaagcgg ttttcgcctg gcgtacccgc ctggcagtta aagacgatga taataagcca
      121 gccgataaac gggattaaga acagcagtgc ccaccacgcc gagcggtcgg tgtcgtgcag
      181 gcggcggaac tgaactgccc accacggtaa aaacaccaga ataccgtaga ttgtcgtcag
      241 gatgccttcg ccaccggcac gttgccagcc taacatttta tccagcagcc ccagcacgaa
      301 cgtaaagatg atgttgacca gaataaacat ccagtactct ttgcgccgcg cacgaccccg
      361 gaaaccgaca taatttttta gtactttcag ataccagtcc atttttgcct caattttgct
      421 caatcacttg tttttaaagc agcaaattaa gaatagacgt gaatgaaatg gcagtaaata
      481 ttcgttcaag gaattaataa agtgccgtaa agcgcgctaa gactgatgac aagcgcaaaa
      541 tcgccagatg cgctacgctt atctggccta cgcggcattg taacatattg aatataaacg
      601 ttttgtaggt tggagaagac gttcacgccg catccgacat aaataacacg cacttagcta
      661 ataatcagag gcacctcgta aggtgccttt ttttaaccga agcgaacatc tcgtccatgc
      721 ggttcatcga gatcctgcca cggaccaatt gaaataatcc ccgtagggtt gatggtctta
      781 tggctgcgga agtaatgatt acggatgtga ttgaaattga cagtttcggc gatccccggc
      841 atctggtaga tatcgcgcag aaaaccgtac agattcaggt aatcgctgat gcggtgctta
      901 tcacacttga agtgggtcac atacaccgga tcaaaacgga ccagcgtggt ccacaggcga
      961 atatcggctt cggttagctg attgccggtc aggtaacgat gctggcctaa aatctgctcc
     1021 agtcgcgcca gcgattcaaa cactttcgcc accgcctcgt cgtaagcttg ctggctggtg
     1081 gcaaaaccag ctttatacac gccgttgtta acggtgtcat aaatccagcc gttaagttcg
     1141 tcaattttcg tttgcagggc tggtgggtag taatcacccg ctttcgcgcc cagcgcatca
     1201 aacgcggtat tgaacatgcg gatgatttcc gctgattcgt tgctgacgat ggtgtggttc
     1261 tttttgtccc acagtacggg aacagtaact cgtccgctgt agtgtggatc ggcgtggaga
     1321 taaagctgat acaaaaattc atgttgatag agtgtatcgc cggttgcacc cggaaaactg
     1381 tcatcaaagg tccagccgtt ttccagcatc agcgggttca ctacagaaac ggaaataaac
     1441 ggttccagtc ctttgagttt gcgcatgatc agcgtgcggt gcgcccacgg gcaggcgagt
     1501 gaaacataga gatgataacg atctttctct gcgataaaac cgcctgtgcc agtggggcct
     1561 ggcgcgccat cggcagtgag ccagttacga aatgcggaag ctgaacgttg aaatttaccg
     1621 ccggtagatt tggtatcgta ccaggtgtca tgccagacgc cgtcaatcag ttgacccata
     1681 tttctctcct ccaatagcaa aaacgaggag cttcctcctc gttttttatc gttaagtata
     1741 gcgcgtgctt accacttttt attcagcagg cggtcgatgc tatacgcgcc cggaccggta
     1801 attgccagca gcaggaatcc gccagaaatt gtcaggtttt tcatgaacat cagcgagttg
     1861 acgccttcag caaagttgct gtgaaataaa aatgccgtca gcagcgtaaa gcccgcagta
     1921 aacagggctg tggtgcgagt caggaaaccg aacaggattg ccagaccacc accaaactca
     1981 agcagaatca ccagcggcag cataaaaccc gggacgccca tcgcttccat atattgttgg
     2041 gtacccgcgt aaccagtaat ttttccccag cctgcggtaa taaacagaat cggcattaaa
     2101 atgcgcgcta ccagtacacc aacatcttct aattttttca tcatactctc cactgcgccg
     2161 ctcaggctgc tgattgtttt tttgtgcaag ggcgcgctat tagcgtcgtt gctgtcgatg
     2221 gagagaatca taaacgtggc gaagcgtgat tgttagcaag gaaaactgtc aaatattttc
     2281 aaaaaatttg agggataagg ccggaatttc tccggccaga gaggaagtta accgcgaagc
     2341 tgttgttgct tgagggttgt tttcaccaga cgccaggcgc tccatacacc aaaaccgcgt
     2401 ctggcccagc ggaccagcat attaggatgg cgaatcgtcc agatagccat cacgctactg
     2461 ccaaccagcg cccaggagcg cagactcaac agcatattcc agcgacgatc gtaagcgcct
     2521 gttgcctcca gccattcacg acgactggcg gaaagatcca gccgttgttg ctggatctgg
     2581 ctaagcagtt gcgccttacg tcgttcacgt tcgactttac tgctcacggg actcctcctc
     2641 gagcagctgc cgatcgtttg ccagctcatg gcgcgtgtgg cgcagcaacg tagacttacg
     2701 cgatttacgt aacgtccaga tgccgccaat cagtgccagt agcagcaaca ccacggtggt
     2761 ggcaatcatc gcattcaggc gatattgcgg atcaaccgcc cagataatta gcaccatcag
     2821 gctcataaga ccaaatgcag cgaaaagcat cgtcaggccc agcatcagta aaagttgaaa
     2881 gagattcgct ttttcctctt ccagctccac caccgccagc cgcagacgtg tctccaccat
     2941 ttcaaccatg atggaaacaa ttcgctgccc gatgcccaga acgcttttac cgggcccttg
     3001 cgcgtgatga gtgtccgcca taattaacga cgcgacagca gaacaccgag cactacaccg
     3061 attgctgcgc caatgcccac gcccgtccac ggattttcac gcacatactc atcggcacgc
     3121 gccgccgcga cacgggtttg tttggcaatg gcatcaccgg tttcacccag gcgataacgg
     3181 ctctgtttca gtgcctgctc cgctttgcta cgaatcttac tcaactcttc tttcgacttc
     3241 tcgccagatg agctaagcac ctcttccagc gtatcggaaa gggatttcaa ctcagcacgc
     3301 agatgttccg tagtgtgttc tttcgacata gttttctcca ggtgagtaag tagtgactat
     3361 tgtgagttag tagtcgcgcg cttccagctt tttcagctct tcctgcgctt ctgccagttt
     3421 ccgttcgcgt ttggcaatct tatctgcatc gcctttttgc ttcgcctcgg ctaaatcttg
     3481 ctggcgttcc gccacctcat ctttctgctt tgcgattttc ttctgatgat cggcacgcag
     3541 ctggctatct gaacagttgg cccggacttc actcagggct ttattcagac cgtcaatacg
     3601 attctggttt tggtgttttt cggcatagct gatctcctta aggatatttt gctccttttc
     3661 ctgacacagg gtagtggcat aactaccggc actaagagca aagagagaaa cagctaaagc
     3721 gatgcggtat ttcattcttg aaccttccat tgtgattcgg acctattaca aaggaacgat
     3781 tgtccagaca acgaagcggt cgtgttaacc accgcctcat taatcataga cagttacggt
     3841 gaaatcacca aagtgagtga tctgtttcgg ataacaggaa gttatccgaa gcgatgagag
     3901 ttatcccgca accgggtcag ccactgcata gcctgactgt tattgtcctg ctgcgcaatg
     3961 atgtagccat ggggcaatgt tctgtcgagg accgcttttg cggcggcgct ctggtcgctg
     4021 gagtcaaagg taattaacag agtgtcgttt ttgggggtga tacttttgaa aggaatgccg
     4081 tgagcgtcaa ggtgatgcca gattgaaaaa ccgtctggca tcgttgtccc ttgatgaacg
     4141 gcacgaatcg ccagcgtaga ctcttgctgg cgaaccgcgg accaggccag caacagtgtg
     4201 ccgaccagaa gtaaaacagc gccggaccag gctagctggc gaagcgacat gcgaggtatt
     4261 tgcatccctt acccccgatt tccatatttc tttttccata acacgaccag agaacctgcc
     4321 aggccaaaca ccagcagcac caccgggagc agcatcaggc atgacatcag ctggtcctcg
     4381 tactttaaaa ataccggcgt tttgccgagc atgtaaccca gagttgtcag gatcaatacc
     4441 cacaacaaac cactcatcca gttgaagaac tgaaagcgcg cgttattcag ccctgataac
     4501 ccggcaatcg tcggcagcag tgttctgaca aacgcaataa agcgaccaat taacagcgcc
     4561 gataaaccgt gtttatgaaa aagatggtgt gcgcgttgat gataatgcgc gggtaaatga
     4621 gatagccagt tttgtacggt gcgggtattg cccagccatc gcccctgaat atagctgacc
     4681 cagcagccga ggctggcggc aacggtcagc agcagaatcg tttgcggata gcccatcgcg
     4741 cctttcgcaa tcaatacacc gaccaacacc agtaagctgt cgcccggtaa aaaggctgcc
     4801 ggaagcaagc cgttttcaag gaacaaaatg acaaacaaga caaaatacaa catgccaatc
     4861 atcgacggat tggccagggt ttcaaaatcc tgcgcccaca gggcttgcag caattgggtc
     4921 aaaagttcca ttcattattc ctggtgaatc ggttaacgcc acgctggttt taaatgatgc
     4981 actaccgcaa tgttattgct gtcttggtat gttctgcggg tttgatacat caacacagcc
     5041 tgaactattc cctgttaaat cgtgagataa gaaagcattc aaaagcataa attcaaattg
     5101 aatctaattg taacaaaggt caacgttgtt cgttatcaaa attacatgtc cgtggagtct
     5161 gattttgctg aatgcagggg agggaggcga gaggatttac acaggctgac actttatact
     5221 tttcgcttac cgacgagaca aacaggcgca ctgtagcgcc tgtcggacag gaatttcatt
     5281 tactgccgct ggtggctgta tcgagatgca ccaccgggtt ttcggcgaac agatagcggt
     5341 cggcattgaa ctcgaaatcg tcgctggttt cgttaaataa catgatcttg gtgttttcca
     5401 ggtgctgcca cattgccagc ttagcagcat gaggatcttt gcgaatcagc gccttgagga
     5461 tttgatcgtg gtcatcacac cagttatcga cggtacgggc atcaatgtgt tcgtgcagtt
     5521 ttttccagta cgggttatga ctacgctggg tccacatttt ttccacgata gccgccaggg
     5581 cggagttctg cgtcgccagg gctacctgaa tgtggaactg caaatcccac tcggaatcac
     5641 ggaagcattg ttcgccgcgc gcctgctcct ggatggccat cagtttcatg atgtcctgtt
     5701 tcgtcacctg agttgccgcg aactcggcaa tattactttc gatgagctgg cgagcctgga
     5761 gcaactcaaa cggaccgtaa ttggcgaatt ccatattatt gtcagtcgcc tgctgatggc
     5821 gcggctggtt ggaaaccaca tgaatacctg aacctttacg cacttcgaca tagccttcaa
     5881 cttccagcat gatgatggct tcacgaacca cggtgcggct gacgttcttt tcatcggcaa
     5941 taaagcgttc agcaggcagt ttatcaccca ccagatagac gccctgttcg atgcgctctt
     6001 tcagatcagc ggcaagttgt tgatacaaac ggcgtggttc agtgatttcc atatgcgctc
     6061 caggcatgat acggcagact atatttgtta taccactttt gcgagtttag ctctactcac
     6121 agtgatgaaa aagccgcctg agaaggcggc tttgaaggga aactaaaact tacacgaagc
     6181 cttaatgttg cggtgcggga tcgttatgtg attcctgcgc cacctcaatc gccggtttgt
     6241 tctgcaacac ggtccagata accagcgcac ctaacaggtc gaacactgcc agaactgcga
     6301 acagcgggct gaagccgatg gtgtcagcca gtgcaccgac taccagtgca aacagcgtac
     6361 ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg ttacgaccga
     6421 acacgtcaga cgagagcgta atcagtgcgc cagacagtgc ctggtgagca aaaccaccga
     6481 tacacagcag cataatcgcg acatacgggt tggtgaacag accgatcata cccgggccaa
     6541 tcatcagcac cgcacccagc gttacgacca tcttacggga aacgatcagg ttcacaccaa
     6601 accaacgctg gaacagtggt ggcaggtaac caccgaggat acaaccgagg tcagcaaaca
     6661 gcatcggcat ccaggcaaac atcgcgatct ctttcaggtt aaagccgtaa actttaaaca
     6721 tgaacagcgg gatccacgcg ttaaaagtac cccatgccgg ttctgccagg aagcgcggca
     6781 gcgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt ttcttcgctg
     6841 tgtcaacctg gtgctgggct tcctgaccat taataatgta gtcgcgttct tcatcggtca
     6901 gatgtttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc cagataaagc
     6961 tcaacgcacc ggagatgata aatgccatct gccagctgtg catcacgatt gcccatacca
     7021 ccagcggcgg cgcaatcatc gcaccaatcg aagaacctac gttaaagtaa cctaccgcga
     7081 tggaacgctc tttcgccggg aaccattcgg agctggcttt cagacccgcc ggaatcatcg
     7141 cggcttccgc ggcaccgacc gcaccacgag caacagccag gccgccccag ctacctgcca
     7201 gcgcggttgc accacagaac acggcccaca gcacagcaaa cattgcataa ccgattttcg
     7261 tacccagcac atccagtaca tagcccgcta ccggttgcat gaccgtataa gcagcagaat
     7321 aggctgcgat gatataggaa tactgttggg tggagatgtt taactcttcc atcagagttg
     7381 gcgcagctgc cgccacagtg ttacgcgtca ggtaaccaag cacggtgccg agcgtcacca
     7441 gtgcgatcat ataccaacgt aaccctttaa ttttacgcat gtaaaacctc atcgttttgt
     7501 tatatccgca ggaagcgcgg ccatcatccc gccagaggct gaagatgtta gttacgaccg
     7561 ggtaaccgaa aaaaaaccgg cacgccccga acgttgccat gaaagtttcg tgctaattcg
     7621 gcttccgtac cggttaaccc gattcatcta acaaatgaat gaatcggcaa tttgcattcc
     7681 gtcgacttat gatttgcgac ggcagaaaga taacttgtca tacaacttta aaaggtgaga
     7741 gccatcacaa atgtgcgaat atttgtaggg acattacctg acgacggcga ggccagtact
     7801 ggcgcggcct gcagcgagat ttaccacttt gagagtaatt tttttaacta cgtttattga
     7861 tctaactcac gaaaatatct tcggactctg gaaattggtg tgataacttt gtcagcatcg
     7921 caccataagc aagctagctc actcgttgag aggaagacga aaatgactcc gtttatgact
     7981 gaagatttcc tgttagatac cgaatttgcc cgccgtctgt atcacgacta cgcaaaagac
     8041 cagccgattt tcgattacca ttgccatttg ccgccgcagc agattgcgga agactatcgt
     8101 tttaaaaacc tgtatgacat ctggttgaag ggcgatcact acaaatggcg cgctatgcgt
     8161 accaacggtg tggccgagcg tctgtgcacc ggtgatgcgt ctgaccgtga aaaatttgac
     8221 gcctgggcgg cgactgttcc gcacactatt ggcaacccgt tataccactg gacgcacctc
     8281 gaactgcgtc gtccgtttgg tatcactggc aaattgcttt ctccgtcaac tgccgatgaa
     8341 atctggaacg aatgtaacga actgctggcg caggataact tctctgcgcg cggcatcatg
     8401 cagcagatga acgtgaaaat ggtcggcacc accgatgacc cgatcgattc tctggagcat
     8461 cacgcagaga tcgccaaaga cggctctttc accatcaaag tgctgccgag ctggcgtccg
     8521 gacaaagcct ttaacatcga acaggcgacc tttaacgact acatggcgaa gctgggtgaa
     8581 gtctctgata ccgacatccg ccgctttgct gatctgcaaa ctgccctgac caaacgtctg
     8641 gatcacttcg ccgctcacgg ctgtaaagtg tctgaccacg cgctggatgt ggtgatgttt
     8701 gctgaagcga acgaagcgga actggacagc attctggcgc gccgtctggc aggcgaaacc
     8761 ctgagcgagc acgaagtggc acagttcaaa actgcggtgc tggtgttcct tggtgccgaa
     8821 tacgcacgtc gcggctgggt acagcagtac cacatcggcg cgctgcgtaa taacaacctg
     8881 cgtcagttca aactgctggg gccggatgta ggctttgact ccatcaatga ccgtccgatg
     8941 gcagaagagc tgtctaagct gctgagcaag cagaacgaag aaaacctgct gccgaaaacc
     9001 attctctact gcctgaaccc gcgcgataac gaagtgctgg gcaccatgat cggtaacttc
     9061 cagggcgaag gtatgccggg caaaatgcag ttcggttccg gctggtggtt taacgatcag
     9121 aaagacggta tggaacgtca gatgacccaa ctggcgcagc tcggtctgtt gagccgcttt
     9181 gtcggtatgc tgactgacag ccgtagcttc ctgtcataca cccgtcacga atacttccgc
     9241 cgcattctgt gccagatgat cggtcgctgg gtggaagcgg gcgaagcacc ggcggacatc
     9301 aacctgctgg gcgagatggt gaaaaatatt tgctttaaca atgcgcgtga ctacttcgcc
     9361 attgaactga actaaggtct ggggttgata tgcaatacat caagatccat gcgctggata
     9421 acgtcgcggt cgctttagca gatttggctg aaggcacaga agtcagtgtc gataaccaga
     9481 ctgttacgct gcgccaggat gttgctcgtg gacataaatt tgcgttaacg gatatcgcaa
     9541 aaggggccaa cgtcatcaaa tatggcctgc cgattggtta tgcattggcg gatattgcgg
     9601 cgggggaaca cgtccacgcc cacaatacgc gcacgaatct gagcgatctg gatcagtatc
     9661 gctatcaacc tgattttcag gatctgcctg cgcaagcggc agatcgtgaa gtgcagatct
     9721 atcgtcgcgc taacggcgat gtcggggtgc gtaatgagct gtggatcctg ccaaccgtgg
     9781 gctgtgtcaa cggcatcgcg cggcagatcc aaaatcgttt cctgaaagag accaacaacg
     9841 ccgaaggtac tgacggcgtg ttcctcttca gccacaccta cggctgctca cagctgggcg
     9901 acgatcacat caatacccgc accatgctgc aaaacatggt gcgccacccg aacgcaggcg
     9961 cagtgctggt gattggtctg ggctgtgaaa acaaccaggt tgccgcattc cgtgaaacgc
    10021 tgggcgatat cgatcctgaa cgcgttcatt tcatgatctg ccaacagcag gatgatgaga
    10081 tcgaagccgg gatcgcgcat ttgcatcagc tgtataacgt gatgcgcaac gataaacgcg
    10141 agccaggcaa actcagcgaa ctgaagtttg gtctggagtg cggtggttct gacggtcttt
    10201 ctggtattac tgctaacccg atgctggggc gtttctctga ctacgtgatt gctaacggtg
    10261 gtactaccgt actgaccgaa gtgccggaga tgtttggcgc agagcagttg ctgatggacc
    10321 attgccgcga cgaagcaacg tttgaaaaac tggtcaccat ggtcaacgac ttcaagcagt
    10381 actttattgc ccatgaccag ccgatctacg aaaacccatc accggggaac aaagcaggcg
    10441 gtatcaccac gctggaagat aaatcgcttg gctgtaccca gaaagcgggt tccagtgtcg
    10501 tggttgacgt gctgcgttac ggcgagcgtc tgaaaacgcc ggggctgaac ttgttaagtg
    10561 cgccgggtaa cgatgccgta gcgaccagcg ccctggcggg tgcgggctgc catatggtgc
    10621 tgttcagtac tggtcgtggc acgccgtatg gtggatttgt gccgacggtg aaaatcgcca
    10681 ccaacagtga actggcggcg aagaaaaaac actggatcga ctttgacgcg ggtcagctga
    10741 tccacggtaa agcgatgccg cagttgctgg aagaattcat cgataccatc gttgagtttg
    10801 ccaacggtaa gcaaacctgt aacgagcgta acgacttccg cgaactggcg atcttcaaaa
    10861 gcggcgtaac gctataaagt cgaaatgagt tgttaataaa aaacggcgtt tcataatgtg
    10921 aagcgccgtt tgcttttcca gacctgctaa ggatttatca tgaccgcgta ttggctggcc
    10981 cagggcgtgg gtgtcatcgc ctttctgatt ggtatcacaa catttttcaa ccgtgacgaa
    11041 cgccgcttta aaaagcagct ttcggtctat agcgccgtta ttggcgtaca tttttttctt
    11101 ctgggcacct atcccgccgg tgccagtgcc atccttaatg ccattcgtac gctgattact
    11161 ttacgcacgc gcagcttatg ggtaatggcg atttttattg tgctgactgg cggaattggc
    11221 ctcgcgaagt tccatcatcc tgtcgaacta ctgccggtaa tcggcacgat tgtcagtacc
    11281 tgggcgctgt tccgctgtaa agggctgacc atgcgctgcg taatgtggtt ttcaacgtgt
    11341 tgctgggtga ttcacaactt ctgggcgggg tcgataggcg gcacgatgat tgaggggagt
    11401 tttctgctga tgaatggtct gaatatcatt cgtttctggc ggatgcagaa aaggggaatt
    11461 gatccgttta aagtagagaa aacccctccc gccatagacg aaaggggtta aacaattaat
    11521 tacgcagggc gctatttgcc agacgatcgt cttccgcctg gcaagctgcc gcagtgaaca
    11581 gcacgtcagt tgaagagttc agcgcggttt cgcaagagtc ctgcaatacg ccgatgataa
    11641 agccgacggc aaccacctgc atggcgatat cgttcgaaat accgaacata ttacaggcca
    11701 gtgggatcag cagcagagac ccccccgcca cgccggatgc gccacaggca cacagagaag
    11761 ccactacgct caacagcagc gctgtgggca gatcgaccgg aatacccagc gtattaaccg
    11821 cagccagcgt caacacggta atggtgattg ctgcgcccgc catattgatg gtggctccca
    11881 gcggaataga aacagaatag gtatcgcgat ccagattcag cttttcacac agcgccatat
    11941 tcaccggaat gttcgctgca gagctgcggg tgaagaaggc atacacaccg ctttcacgca
    12001 ggcacagcag caccagcggg aacgggttac gacgaatttt ccaccacacc agcaatgggt
    12061 taaccaccag cgccaccagc agcatacagc caaccagcac gaccagcagt tgcgcgtagc
    12121 cccacagcgt ggagaaaccg gtggttgcca gggtagaaga aaccagacca aaaataccga
    12181 tcggtgcgaa gcgaatcacc agtttcacca taaaggtgac ggcattcgac atatcgttga
    12241 tcaggttttt ggtggtctcg ttaccgtgac gcagtgcgaa accgaggcca attgcccaca
    12301 ccaggatccc gatatagtta ccttttagca gcgcgtcaat ggggttggaa accatgctca
    12361 ttaccagccc gcgcattact tctacaatgc ctgacggcgg cgaaatatca cccgcgctac
    12421 tggacaagtg cagggtagaa gggaaggcaa aactgaagat tactgcggcc agagcagcag
    12481 agaaggtgcc cagcagatag aggaacaaaa tagggcggat attggttttc tgcccgtgct
    12541 ggtggttagc aatagatgcc attaccagca tcaacaccag gatgggggca acggctttca
    12601 gtgcgccgac gaacaaagta cctaacagac caacagcttc cgccgcgggt tttgagatcc
    12661 atgccagaag aatccccaga acaaggccga ccaggatttg ttttaccagg ctgccatgag
    12721 ccagacgccg gaatagcccc ggtgaacgtt gcgtagtcat ttttcgatcc tttcattgtg
    12781 ttgtcgcaca tccttggtgc gttctgtcga cgcatctttc aggatgtaac aaagtgtttg
    12841 caaggtcgag tataaggaaa gttacagctt cgggaagcga aaaatgctgg attttaaaga
    12901 gaggtcatat tttgcaactt aatggttaca tttatttgac ataaacagag ggaaggactg
    12961 gctactaagg ctgattagat aacatatggg caaaactatt tggaatatga gaacggttgc
    13021 gctcctttaa tgtgctatgc catataaagc cactggcgca gggagcgcgc agggggcggc
    13081 caatcgccgc cgccccctgc acccccaggc tctggcgaac aaaatcgccg ctgcgcggtt
    13141 ccctcagctt atcccttgcg gctaccgggt cgggcgcaag ggaacgtccc tgtaagatgc
    13201 gccctcgccg cgcatccatg cgcgtcgccc cggcctccag ggaacgcttc ggcgattttg
    13261 acgccaccaa cacccgtgcg gtctttttgt aaagagctat aagccgatta cataattaat
    13321 agctcacctt tgtctggaat gagttatctc tagtaatggc ttaatataca gcgaatcatt
    13381 ctccccgctg cttatcatgc cgataattca cccaggcgtt gataataaac gtcatcacca
    13441 gaatgccaaa caccacgccc agcgagacgg cgatcggaat atggtagaag tcgacaatca
    13501 gcatcttgat accgataaac accagaatca ccgccagacc atatttgagc atcgagaaac
    13561 gctctgccac acctgccagc aggaaataca tcgcacgcag gccgaggatc gcaaacaggt
    13621 ttgaggtcag cacaataaac ggatcggtgg ttacggcgaa gatagccgga atactatcca
    13681 cggcgaaaat cacgtcgctc aactccacca gaatcagcac cagcatcagc ggcgtggcat
    13741 acaacaaacc atttttacgc acaaagaaat gctcgttgtc gatggtgtcg gtcatgcgca
    13801 aatgaccgcg tagccagcgc accagcggct tgtcgccaat gcctgattca tcttcatggg
    13861 cgagcgccat cttcacgccg gtaaacagca ggaaggcacc gaagatatac aggatccagt
    13921 cgaactgtga aatcagccag ctgccagtga agatcatgat ggtacgcaga acaatcgccc
    13981 cgagcacgcc atacaccagc acgcggcgtt gtaatgccgc cggaacagag aaatagctga
    14041 acaacatcag ccagacaaag acgttatcga ccgccagcga tttctcaatc agataacccg
    14101 tgagaaaggc cagtgcctgt ggatcggcaa cggcgcgacc ttcggtttgc accagatacc
    14161 accagaaagc ggcgttaaac agtaacgaca gcgtcaccca gaccagcgac caggccgcag
    14221 cctgtttcat ggtcatggca tgtgccccac gacgcccctg caacaacagg tcgatagcca
    14281 gcataatggt gacaacaaca gcgaatccgc cccatagcaa cggcgtgccg acagtattca
    14341 tagaagttcc ttacacataa aaaaacggcc aacgtcggaa gacgtcagcc gctgcttttt
    14401 atgcatagac ctcgccttcc ggcaaggtct cacttacaac aaaaaaggaa tacgtctgcg
    14461 tattcctttc gggttgcccg gtgaccggat gtggtttttc acacatcgta atgacgatcg
    14521 accggcaatg aagttactcc cctttgcagg taacaaagta tgacagacca ttcagtcagt
    14581 caatggtctg ttacatacct tttacactgt tttactttgg caattatagt tttacgctat
    14641 ctgccggaaa aatcacccca gtctggcggc ggatctcggt cagcagttta gcggtgatgc
    14701 gactgaccgc cagccctgga tgatccacca gatgctcatc caccagttca gcaaacagct
    14761 ctgcttcgta gagcatggta ttaatatgct gcggttgggt cagatcctgc atctggctgc
    14821 cacgcggcac aaaacacact ttctggcatt cagacagttt ttcaatcacc agcgatcccg
    14881 cttcgccctg aatctcgctt gccaggacag aatcgctcac ttttgagtgc tgcaacgtaa
    14941 cgctgaaatc accgtaatcc atcaccacca cgccgtgggc atccacaccg cttgccagca
    15001 aactggcggt tgcctgcacg tttttcggtt caccgaataa tgccaccgcc gacgccagac
    15061 agtaaaagcc gatatccata attgaaccgt tagagaatgc tggattaaag gtgttgggat
    15121 tctcaccatc caggtaacgt tgataccgcg aggaatactg gcaatagttg aaaaagactt
    15181 tacgcagttt gccgactttc ggcagcgcct ggcgcaacag atgaaagttc ggcaggtagg
    15241 ccgttttaaa tgcctcaaac agcaccacct gattttcccg cgcacaggca atggcggcat
    15301 ccacttccgc cagattcgat gccagcggct tctcgcaaat cacatgaatt ttgtgactaa
    15361 ggaaaagttg tgtctgggaa aagtgcaggg cattcgggct ggcaatatac accgcgtcaa
    15421 tggcatcgct ttccgccatc gcttccagcg acgtaaacag atgctcgaca gaaaaatcat
    15481 tggcaaagtg ctgggcctgt tcaaggctgc gggaatatac ggcggttaac ttgtatttac
    15541 cgctctcatg ggcggcctcg acgaactggc gagtgatcca gttcgtacca atcacagcga
    15601 aacgtatcat aacgcgtgga tactccataa agggattctg ccgtcaatgt agcacgcctt
    15661 tttcatcttc gacgtgcgca gatacgcatt atttctgcgc cggacagcca tgctcactaa
    15721 acggcccaat catgaccgat ggcccgagat atttcggctg gcgatggagg atttttgcgt
    15781 caatcaccgc gccgacgcga gcgagtttct cccgcagttg agtataaaaa ccgtgcttca
    15841 tattgaggtc tggcgcgtta aaaccgatag cgtcgatgcc gtactgtttt gccagccaga
    15901 tggcgcgttg gttatgaaat tcttgcgaaa taatggtgat atggttttcc ccaaacacct
    15961 ttttggcacg caccaccgaa tcaagcgttg agaatccggc atagtcgcag aagatcactt
    16021 tcgccggtac gcctttagcg atcaaagcct gctgcatacc tgaggcttca tcgtaatttt
    16081 tacgcccgtt atcgccactt accagtagcc atttcacttt tccggcgtgg tacaacgctg
    16141 ctgcggtatc aatacgtcgg gtaaagtagc gattacccgg cctcgccccc agcaataaac
    16201 ccacgttacg cgccggaact gcgctgacat cgctccaggt cagctgcttg ctggtgttga
    16261 ccataacgcg atcggcaata aaaatggtcg ctcctgctat aagcaacaat aaacagccaa
    16321 ttttaagagt ccggcgagaa aaacaaatac ggagaagaag gcgggcctgc atggtcgtta
    16381 tccttaacga aaggggggag gtcgatttta ggcgctatag gaagaaaaga taacgcctga
    16441 catcattttc agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg
    16501 aactcaagct ggtgatactg cggctccata tggcagcaca attgatagaa cgctttgtta
    16561 tgctctttct ccttcaggtg cgccaactcg tgcaccacga tcatgcgcaa aaacggttcc
    16621 ggcgcgttgc gaaacacggt agcgacgcgg atctccgcct ttgctttcag cttgccgccc
    16681 tgcacacgcg aaacggcagt atgtaatccg agcgcgtttt tcagcacgtg gatcttattg
    16741 tcatacatca ctttagtgat cggcggggca ttgcgcagaa actgattttt aagatcctga
    16801 gtatattgcc agagggcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc
    16861 agcacatctc ccagacgctg ttcgttaatc agcgttcgca cctgggaaag tagctgctcg
    16921 ggataaccct gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt
    16981 atactcacgc acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga
    17041 gccacttaga caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg
    17101 ttaacccact acaggcgtgg gaagcggcgg atgaatattt gctgcaacag ttggacgaca
    17161 cagaaatccg cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agttgtgcgc
    17221 tggcggaaca taagccatac agcattggcg actcatacat cagcgaactg gcgacgcgcg
    17281 agaatttacg cctcaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg
    17341 actacccgca acagccgggc gtggtactga tcaaagtacc gaaaacactg gcgttgctgg
    17401 aacaacaact gcgtgcgctg cgcaaagtgg tcacgccgga tacacgtatt attgccggtg
    17461 ccaaagcccg tgacatccac acttccacgc tggaactgtt cgaaaaagtg ctcggtccga
    17521 ccaccaccac gctggcatgg aagaaagcgc gcctgattaa ctgcaccttc aacgaaccgc
    17581 cgttggccga tgcgccgcag accgttagct ggaagctgga aggcactgac tggactatcc
    17641 acaaccatgc gaatgttttc tcccgcactg ggctggatat tggcgcgcgc ttctttatgc
    17701 aacatctgcc agagaatctc gaaggtgaga ttgtcgatct cggttgcggt aatggcgtta
    17761 ttggcctgac attgctcgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcac
    17821 cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa catgcccgag gcgttggatc
    17881 gctgcgagtt tatgatcaac aacgcgctct ccggcgtgga gcctttccgt tttaatgctg
    17941 tgctctgcaa cccgccgttt catcagcaac atgcgctgac cgataacgtc gcctgggaga
    18001 tgttccacca tgcccgccgc tgcctgaaaa tcaacggcga gctgtatatc gttgccaacc
    18061 gtcacctgga ttacttccat aaactgaaga aaattttcgg caactgcacc accatcgcca
    18121 cgaataataa atttgtggtg ctgaaagcag tgaagctggg gcgtcgtcga taagattggg
    18181 agttacctgc cggatgcggc gtaaacgcct tatccggcct acgttcagca cattaccccc
    18241 aggtcggata agacgcggca agcgtcgcat ccggcaatgt ctgcccagtt ctggaatcgc
    18301 gcttccataa aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt tagcgttttt
    18361 ctacgtttat gctacatacg tgaagttatg acgcataaag aatacgattt ctttaccgcc
    18421 gttcatcgta ctaaggggaa aaaatgattg cgattgccga catcttgcaa gcaggagaaa
    18481 agctaactgc tgtggcacct tttctggcgg gtattcagaa cgaggaacaa tacgcccagg
    18541 cgctggaact ggtcgatcat ctgctgctca acgatcctga aaatcccttg ctggatctgg
    18601 tgtgtgccaa aataaccgcg tgggaagaat cagcgcccga atttgctgaa tttaatgcca
    18661 tggctcaagc catgcctggc ggtatagccg tgattcgtac ccttatggat caatatggtt
    18721 taaccctttc cgatcttccg gaaattggca gtaaatccat ggtgtcacgc gttttgagcg
    18781 ggaagaggaa attaacgctg gaacacgcta aaaaactggc aacgcgattc ggcatttctc
    18841 ccgccttgtt tattgattaa cgcgacgtgc cggatgcgtc gcatccggcg cttcaaacaa
    18901 cgatttaaat ctccagcgcc agccgtgttc cctgggcaat tgcccgtcgt gcgtcaagct
    18961 ccatagctac atcgcagccg ccaattaaat gcacagtttt cccgctatca atcagctgtt
    19021 gcgccagcgc gcggtttggc tcttgccctg cgcagatcac cacattgtcc actgctaata
    19081 cctgcgtttc gccgttgatc accacatgca gcccgtcatc gtcaatcttc tgataactta
    19141 cgcctgggat cattttcaca ccccgcgaga gcagggtggt gcgatggatc cagccggtgg
    19201 ttttgcctaa cccctgtccc ggtttgctgg ctttgcgctg gagcatcaca atctgccgtg
    19261 ggctacgcgg gatctgcatt ccctgcgggc ttaagccacc agcctgttgc aggctgctgt
    19321 cgatccccca ctcattacag aactcggcaa tgttctggct ggtggactca cccggctgac
    19381 ttaaaaacat cgccgtatca aaaccaatcc cgccacaacc gatgattgct acctttttgc
    19441 ccacaggcgc tttgtcgcgc agtacgtcga gataactcaa caccttcgga tgatcgattc
    19501 cgtcgatagg tggaatgcgc ggtacgatcc cgctagcgag gatcgtttca tcgaacgact
    19561 gtaactgatc cgccgtcacg gtgtgattga gtttcagcgt cacgcctgtt acttcgatca
    19621 tccggcggta atagcgcagc gtttcgtaaa actcctcttt gccagggatc tgtttggcga
    19681 tattaaactg accgccgatc tcactgtgag catcaaacaa cgttacctga tgaccacgtg
    19741 ccgccgcgtt aatggcaaac gccagcccag ccggaccagc gccgaccacc gccagatttt
    19801 ttttctgcac ggcgggaagg atcggcattt tggtttcgtg gcaggcgcga ggattcacca
    19861 ggcacgaggt gactttgcca acgaagatct gatcgaggca ggcttgattg cagccaatac
    19921 aggtgttgat ctcatcggct cgccccgatt gcgcttttga cagcagttcc gcatcagcaa
    19981 gaaacggtcg cgccatcgat accatatcgg catcaccgcg cgagagaatg tcgtcggcaa
    20041 cctgcggatc gttaatccgg ttggtggtca ccagcggcag cgagacgtgg cttttcagtt
    20101 tgcgcgtgac ccagctaaag gcaccgcgcg gcacgggcgt ggcaatggtc ggaatacgtg
    20161 cttcatgcca gccaatgccg gtgttgataa tggtcgcgcc cgccgcttca atggcctgcg
    20221 ccagccctac cgtttctgca aaagtaccgc cgccttctac caggtcgagc atcgacagac
    20281 ggtagataat aatgaagtca ttgccgacgc gttcgcgcac tgctcgcacc acttcaactg
    20341 caaagcgcat ccggttgcgg taatcgccgc cccactggtc gctacgctgg ttggtgcgca
    20401 gggtcagaaa ctcgttgatc aaataccctt cggaacccat cacttctacg ccgtcgtatc
    20461 cggcctcccg cgccagttgc gcgcagcggg cgaaattgtc gatcagttgc aggatctctt
    20521 catggcttaa ctcatggggc acgaaacggt tgatgggggc ctgcaatgcg gagggagcga
    20581 ccagatgcgg ttggtagctg tagcgcccgg tatgcaaaat ttgtaaggca attttgccgc
    20641 cttcctgatg tacggcttcg gtaatggtgc gatggtgtgg gatctggctg gcgtcgttga
    20701 gcattgcgcc gccttccatg ccaacgcctg ttaaatctgg tgcaatgccg ccgctgacaa
    20761 tcaacgccac gccatgacgg gcgcgttccg cgtaaaacgc tgccagccgt tcagcgccgt
    20821 ccgggtattc ctccagtccg gtgtgcattg aacccatcag tactcggttt tttaacgtgg
    20881 tgaagcctaa atccagcggg gcgaacagcg acgggtagct cataaaattg tccagtatgt
    20941 aaaataattg ttatgtggtc ggatgagttc taatttagcc gtcgctggag aaaagggaaa
    21001 aggggagtgc ggggtttgtg atgggattca aaaagaatac gcccgttcgt catgccggac
    21061 ggcggtaata cagtcgtccg gcatcgggaa aaattactgc ttacggaaaa aatcgttata
    21121 caacatatac aaatgcgcgg cactccagga gaaatttggt gcgccttgct gtgcgccagt
    21181 cagcgggttg taattctcct gaattgggcc atcggcggtt aatcctttgg cgtgccggaa
    21241 gaacgtatcc gccagcttca gggcatcatc gcgataaccg taacgctcca tccctttcag
    21301 accaaaccag aactgatcca cccacacgcg cccgcgccag taaatatctg cgccaaaggc
    21361 cggattggtt aacgccgccg ttcccagcgg tacaaaggtg ttgaactctt taggatcgag
    21421 catcaccttc accaccgcgt cggcgttggc ctgcgttgcc gcaccgttaa acagcggcga
    21481 ccagccttcc ggccctttgc cgcgctcgac aatcggtttg cccgcgcagc cgttcgccag
    21541 tggtttatct tcaatgcgca cgtcatagta gaactgggta gtcgggtcga acatacaggt
    21601 gttgatgtag tccgcgagct gttgtgctaa ctgacgatag cgtttggcct cttccggctt
    21661 accgagaatc gtcgccatct ccgccagata atggttatcg ctgtacatat agctggcctg
    21721 atccaccgac tcctgcaata gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc
    21781 ggcgaatttc accgtccagt cgctacgttt gccgccgtta gcgacatatt tatccagctg
    21841 ttctttgtcg ataaacccaa agacggcggc atcatcacgc cccgattccc acgacgccgc
    21901 aacctgtgcc gggatttcca gactgtcgta ctggcctttc tctaccacgc gggcgtagtt
    21961 gttcagccca gacagcgtct ctgctttgtt gcctttcttc accgtaaaca gcatctcgcc
    22021 gctctcagtg ttgtgggctt tgtcgcgggt cgcgccatat tccggcacgc cgttgccgtt
    22081 gtgatcgcgg ttacgtagcc accagtcgtg ataggccacc agtttcgggt acatctctgc
    22141 cagccaggtt ttatcctggg tgacgttata cacttccatt actgaccagg cggcaaggct
    22201 gggtttggta ttacgtttgt tccagttgcc gccatcgccg ccacgctcgg ggctaaggtt
    22261 ccatgctatc aggtcgggga caaagcccac atcctgcgga cgcacgcggt cgcctggctg
    22321 gatctgccag gagaagaccg cgcggatatt ctctttggcg atatccggat tgaaatgcgc
    22381 catcgcaaat gcctgcttcc aggtatccca cggccaggtc tgattgccgg agaaccagcg
    22441 cccggtcacc gacggcgtga cggtgttata tttcaccgcc cccccaggcg agcgccagtt
    22501 accgttgagc gtttcgatcg ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg
    22561 attggttaac cctttcttca gatactcttc ccagcgttgc tgcgaggcgg tgagataaaa
    22621 cgccggacgc gccagaatat cgcggatctg catttgctct ttgctaactt cctgagcggt
    22681 cagcaggtgg gagtaggtgg tatagagcgt ggccgaaccg ttgatatgcg ccttactggt
    22741 aaagcgatta ccgttgattt cagtctgcac tggcagggat ttatgcacct gatattctga
    22801 ttcaccggag gtcagcagat cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc
    22861 atcacgggtg gcgctgattt tgcgctgata gtcagggtat tcgccagcaa tggttttatc
    22921 ggaaagcggt ttcccttctt tcgcttccag tttttccagc agctcgccgt cccacaccag
    22981 atccagcggt ttattgctga tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa
    23041 gcgcagagtc atttcgacct gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg
    23101 aatactgtat gcctccagcg tgaagtcgac tttcttgccg tcttgccaga cggtcagacg
    23161 atcgaaattg ctggccataa agttgatgta ttcttccgtc agcagcgcaa cgcctggaaa
    23221 accgcccatg gtgttcgggc cgtctggcaa cagatgacca tgccaggctc cgagatcgaa
    23281 aaacggatta aaacgctggt gatcgtcgta atcgtaatcc ttcatgtact gcggcgcgcc
    23341 agtacggtta atcacgtttt tataattgtc ggcgttagcg gcatgggcgg aaaaacttat
    23401 cagcagagcg caggttactg gcgttaaaat agttttgatt ttcatgaaat tacagtcctt
    23461 attaccagta gaaatattgc atcaggaaga gttggtcgct gggatcgtga gaggcatttt
    23521 tcatgtagaa gcggctatca aaagccgttg cgaaacgtcc gccgccgtat tcgtaccaga
    23581 taccaaactg catgaaagag gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt
    23641 aggtgtaagc ggtggagaga taaatgcctt ttaacgcttc atacatggcg ctcaccatca
    23701 tcccgctttc acttcccgat acgtccagcc cgtcggcgcg tccggtatgg tgccaggcgc
    23761 gagcggcaaa gttaggggta agcaggcccg tcagatagag ttggcctgtg ccgtcggtga
    23821 tttccagacc gttcatccag gtgatgcctt cggcaaggtc atactggatg taaccgttca
    23881 tcattgccgg ataggtgtat ttgtcgtcgt agcgatcgta cttgccaaaa tgtagccagg
    23941 ctttgctttc gtcatgatgt ccggcaggtg tggcggtaac gctataacga aagttgccgc
    24001 ttaagttctg caccttcagc gcggtcataa tgtcgcgggt attgggaatg acgtagccca
    24061 gatcgggcgt gaaatcgccc caccattgca ggtcatccag cgaggaatcc tgacgcagac
    24121 tgagcatgat ttcggtgcca tcattggtac gataaccgcc atagaaacgg ttaatcccac
    24181 cttcgaaacc gccccagcta tggtcaggca cccaggcatt accgtcgtgg tcggcctgca
    24241 cggtccagcc ttcgccgtaa atcaggccga accaggatcc gtacttcatc tccaggccac
    24301 cttcaatata ggtgccgtcg ctgtatttat gtttattctc accctctaaa tccatatacc
    24361 caccaacccc taattcgccg taaaaacgaa atgaaggaga agatgtcgag gtcattgcgg
    24421 gggtttttgt gtcgtcatgt tctgccggac gtgcttcatc agcggtcaca gaaaaggcgt
    24481 agcaaaacgg cagcgcaagt aatgctctgc atggtgcagt gattaatttc attgtgttaa
    24541 tccatgagct aaagaaaaag tacagccttt tatcgttgta attataaaaa tataaaggc
//
LOCUS       DAZKGF010000070        24379 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZKGF010000070 DAZKGF010000000
VERSION     DAZKGF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.070"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1200)
                     /gene="yadE"
                     /locus_tag="ACHOQV_002589"
     CDS             complement(<1..1200)
                     /gene="yadE"
                     /locus_tag="ACHOQV_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9630948.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYTTLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMS"
     gene            complement(1264..1704)
                     /gene="yadI"
                     /locus_tag="ACHOQV_002590"
     CDS             complement(1264..1704)
                     /gene="yadI"
                     /locus_tag="ACHOQV_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9630949.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMLV
                     YRETLSSAEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(1809..2579)
                     /gene="yadH"
                     /locus_tag="ACHOQV_002591"
     CDS             complement(1809..2579)
                     /gene="yadH"
                     /locus_tag="ACHOQV_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9630950.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(2576..3502)
                     /gene="yadG"
                     /locus_tag="ACHOQV_002592"
     CDS             complement(2576..3502)
                     /gene="yadG"
                     /locus_tag="ACHOQV_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9630951.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            3611..4273
                     /gene="can"
                     /locus_tag="ACHOQV_002593"
     CDS             3611..4273
                     /gene="can"
                     /locus_tag="ACHOQV_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFP9630952.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(4314..4850)
                     /gene="hpt"
                     /locus_tag="ACHOQV_002594"
     CDS             complement(4314..4850)
                     /gene="hpt"
                     /locus_tag="ACHOQV_002594"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP9630953.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            5056..7446
                     /gene="gcd"
                     /locus_tag="ACHOQV_002595"
     CDS             5056..7446
                     /gene="gcd"
                     /locus_tag="ACHOQV_002595"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFP9630954.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILAWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKNLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(7642..9192)
                     /gene="cueO"
                     /locus_tag="ACHOQV_002596"
     CDS             complement(7642..9192)
                     /gene="cueO"
                     /locus_tag="ACHOQV_002596"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFP9630955.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     VIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            9358..9705
                     /gene="yacC"
                     /locus_tag="ACHOQV_002597"
     CDS             9358..9705
                     /gene="yacC"
                     /locus_tag="ACHOQV_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFP9630956.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            9811..10677
                     /gene="speE"
                     /locus_tag="ACHOQV_002598"
     CDS             9811..10677
                     /gene="speE"
                     /locus_tag="ACHOQV_002598"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFP9630957.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            10693..11487
                     /gene="speD"
                     /locus_tag="ACHOQV_002599"
     CDS             10693..11487
                     /gene="speD"
                     /locus_tag="ACHOQV_002599"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFP9630958.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITTALWKEMREIYYGRNMPAV"
     gene            complement(11525..11887)
                     /gene="yacL"
                     /locus_tag="ACHOQV_002600"
     CDS             complement(11525..11887)
                     /gene="yacL"
                     /locus_tag="ACHOQV_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFP9630959.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(12062..14659)
                     /gene="acnB"
                     /locus_tag="ACHOQV_002601"
     CDS             complement(12062..14659)
                     /gene="acnB"
                     /locus_tag="ACHOQV_002601"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFP9630960.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            15014..16771
                     /gene="yacH"
                     /locus_tag="ACHOQV_002602"
     CDS             15014..16771
                     /gene="yacH"
                     /locus_tag="ACHOQV_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFP9630961.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQEVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPTNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHSAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSDHTVRLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(16842..18266)
                     /gene="lpdA"
                     /locus_tag="ACHOQV_002603"
     CDS             complement(16842..18266)
                     /gene="lpdA"
                     /locus_tag="ACHOQV_002603"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP9630962.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(18591..20483)
                     /gene="aceF"
                     /locus_tag="ACHOQV_002604"
     CDS             complement(18591..20483)
                     /gene="aceF"
                     /locus_tag="ACHOQV_002604"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP9630963.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAASAPAAGVKDVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(20498..23161)
                     /gene="aceE"
                     /locus_tag="ACHOQV_002605"
     CDS             complement(20498..23161)
                     /gene="aceE"
                     /locus_tag="ACHOQV_002605"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFP9630964.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPTMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(23322..24086)
                     /gene="pdhR"
                     /locus_tag="ACHOQV_002606"
     CDS             complement(23322..24086)
                     /gene="pdhR"
                     /locus_tag="ACHOQV_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFP9630965.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         5829 a         6668 c         5985 g         5897 t
ORIGIN      
        1 cgacatcgtc tcgagcgaat ccgttcttaa gatataaagt cgttttagta acaacggatt
       61 atcccccggt tttactttgc ctttcatggt tgtcaccgcc aggtgaaatc cggcttcttt
      121 tgctgccttc acggctttgt cattaaatcc gccaaacgga tacgaaagat accagacatg
      181 cggattaaat tgcgccagag cgcggcgtga acgtgcaaaa tcaaacagaa tattgtgctc
      241 actacggctc agcaatatgg gtcggcgata accatccacc cgatgcaaaa aatgggtatg
      301 tgactggaaa tcaaatacat cgcgaatttc gttaagttca gaaacgctca taaattgcag
      361 cgattttggg tcccacttct gcgggtgacg tttgatgcgc gaggtaacaa taaacgccgt
      421 cgccttcatg ccatattgtt tcaacacagg atacgcatag cggctcaccg atttgaggcc
      481 atcatcaaag gtaatcacca ccgctcgcgc agggagattg atcttattct tcacgtaacc
      541 ttccagctgc accatactca gtgtcgtgta tcccctgtcg cgcagccagg ccatctggtt
      601 attgaaagcg cgtaccgagg tggtcgtcga agtatggcga aaacgggtgt tttcttcgtc
      661 gcgcagaata tggtgatagg ttagcaccgg caggccatta tcgggttggg catccagcgc
      721 gctgatatag gccagtcgat cgccaatacg gatctgatac caggtttgat ttaacctgtc
      781 tttcagttta tgcaaaatcg ggtagcgcaa attgtccgcc agtaccccaa acggcgcact
      841 gcccgcactc ggcgcgttat agaccggcgt atctttccag gtaactaagt tctgattact
      901 cagcggcttg ttgaggtcac ccaaaccgtc ttcaactttt tgtcgcccct gaaccggctc
      961 gagatgacct ttatcgataa accctttacc aaagccaaaa ttaaatgcgt aataacttgc
     1021 ggcagtgggc tccacggcaa tgatttgccc ggcccgaata ttccccacgg tcaccatctt
     1081 gtcgccaatt tgcgcccaga ccgcagcatt ttcgatggtt tgcatataac gggcaggtaa
     1141 cgcggcactg acgcttgcgg taaacagcat cagcaggaga ataacagctt gtttgtacat
     1201 gaaaaaataa ccgagggtga aacaacgctt ttattgtagc aaaagcgtca tcagaatcgc
     1261 gggttaatac tcatacaaat tatgtttgag gacgaaaggc ggattttttt gttgctggtg
     1321 ccaaagacta ctcacctccg gcgcacccag ttcgacaata cgctcgcgaa actctgcgct
     1381 ggataacgtt tcacggtaga ccagcatctg ctcaattaac ggtaacgtga cgccagaaat
     1441 cacttcgcaa cgggaatgtt tatggcttaa tagcgaagcc acgcggtacg gtggcgctcc
     1501 ggctatatcg gttaagaaga tgacaccttc accagagtcc gcttcatgca gagcatcgca
     1561 catcatgcgg ctgagcatat tagagcttaa cccacgccag aaattcacgg cccggcactg
     1621 aagaagtgcc ccatgttttt tctccagcgc atccagtatc tcttgcgccc ggtcatcgtg
     1681 acaggtaatt acccaaccta acatcgcctt cctccttagc cgaaaatcag tctaccctga
     1741 ccattgttga taaagggtga tagccgtcaa aactcctctc ccccaaatcc agaggagagg
     1801 aaatagcctt agctacgcaa accacgtcca cgttggatca gcgaccaaca gatcaaataa
     1861 aacgccacaa taaagaccac cagtacaccg aaggtagtga ccagcggaac atcattgata
     1921 ccgaggaagc cgtagcggaa accactgatc atataaacga ttgggttcag gtgcgacagc
     1981 ccttgccaga acggcggcaa caaagtcagt gagtaaaaga ccccgcccaa atacgtaagt
     2041 ggcgttaaca caaaggttgg caccaggctg atgtcatcga acgttttggc aaacacgccg
     2101 ttcagcaaac ccgcaaggga gaacaacacc gccgtgagca ccagcgttaa agcgacaaat
     2161 acccacgaat gcacctgaaa cggcacaaaa aacagtgaaa ttgccgtcac cagaatgcca
     2221 acaaacagac cacgcgccac gccaccgccg acatatccgg caataatgac gtgagtcgga
     2281 accggcgcta ccagcagctc ttcaatatta cgctgaaact tggcaccaaa aaatgatgac
     2341 gcgacgttag cgtaggcgtt ggtgatgacc gacatcatga tcagccccgg tacaatgaac
     2401 tgcatgtagc tgaagccatg catatcgcca atacgcgaac caatcaggtt accgaagata
     2461 ataaagtaaa gggtcatggt gatgaccggt ggcaccagcg tctgcaccca gatacgcata
     2521 aagcgatgga tctctttcgc ccagatgctt tttagtgcca cccagtaaag atgcatcatg
     2581 cgcgatctcc ttgtttttca ttaaccagtg aaacaaacag ctcttccaga cggttagctt
     2641 tgttacgcat acttaatacc tgaatgccct gctcacttaa ctgcgtaaat acgctgttga
     2701 tcccctgctc acgcagaact tcaacttcca gcgtcgcggt atcgaccagt cgatactgat
     2761 agccatcgag cttcggtaac gggcttttcg gtgcgagatc gagaataaag gtttccgatt
     2821 tcagcttcgc cagcagcgcc ttcatcgagg tattttccac cagctcaccg tgttgaataa
     2881 tgccgatatt gcggcacagc atttctgctt cttccaggta gtgtgtggtg agaatgatag
     2941 tggtgccttt gtcgtttaaa tccttcaaaa agccccacat tgagcggcga agttcgatat
     3001 ccacgcctgc ggtcggttcg tcgagaatca gtagtttagg ttcatgcatt aacgcacggg
     3061 caatcattaa acggcgcttc atcccgccag ataacatacg cgcacgttcg ttgcgttttc
     3121 cccatagatc gagttgttta agatactttt cgctgcgaat gtacgcttct ttgcgctcca
     3181 caccgtagta ccctgcctga ttcaccacaa tttgctgcac ggtttcaaac gggttgaagt
     3241 taaattcctg tggcaccaga cccaactgac gtttagcgtt tacgacatct ttctcgagat
     3301 cgtaaccaaa tacgctgacc cgcccggagg ttttatttac cagagagctg ataataccga
     3361 tagtggtcga ttttccggcc ccgttcggcc cgagaagcgc ataaaaatca cccgcttcga
     3421 cctgcaaatc gattccacga agcgcctgaa cgccgcctgg ataggttttt ttaagctgtt
     3481 gaagttccag tgcaatggtc ataaattttt acttacctta cgttcttaca ctttatctgt
     3541 ggtttaaatc gtcccggagt tgccctatat tagccaaacg taattatttg gttacaggtc
     3601 gttaacctcc atgaaagaca tagatacact catcagcaac aatgcactat ggtcaaaaat
     3661 gctggtggaa gaggatcccg ggttttttga gaaactggca caagcgcaaa aaccgcgctt
     3721 tctatggatt ggatgttccg acagtcgcgt tcctgcagaa cgtttaaccg gtcttgagcc
     3781 gggcgaactc tttgttcacc gtaatgttgc taacctggtc attcacaccg acctgaactg
     3841 cctttccgtg gttcagtatg cagtggatgt actcgaagtt gaacacatta ttatctgtgg
     3901 ccactacggt tgcggcggcg tacaagccgc agttgaaaac ccggaactgg ggcttatcaa
     3961 caactggctg ctgcatatcc gcgatatctg gttcaaacat agctcattgc tcggcgaaat
     4021 gccgcaagag cgccgtctgg ataccttgtg tgaactgaac gtcatggaac aggtgtataa
     4081 cctgggccac tccaccatta tgcaatcagc gtggaaacgc gggcagaaag ttaccattca
     4141 cggctgggcc tacggcattc acgacggctt gctgcgtgat ctggatgtca cagcgactaa
     4201 ccgcgaaacc cttgagcaac gttaccgtca cgggatctcc aacctcaagc tgaaacacgc
     4261 caaccacaaa taaaaatgcc atgccggatg caacacatcc ggcaacttca cacttactcg
     4321 tccagcagaa tcactttgcc gatatacggc agatgacggt aacgctgtgc gtaatcaatg
     4381 ccgtaaccca ccacaaactc atccgggatc gagaaaccga taaattctac cgggacgttc
     4441 acttcacgac gggacggttt atccagcagc gtacaaatcg ccaacgactt cggttcgcgc
     4501 aggcttaaga tctcacgcac tttcgacagt gtattccccg agtcgatgat atcttcaaca
     4561 atcagcacgt ccttgccacg gatatcttca tccagatctt tgaggatttt cacatcacgg
     4621 gtggtggaca tgccgctacc gtagctggag gcggtcataa aatcgacttc atgagatacc
     4681 tgaacttcac ggcacaggtc cgccataaac ataaatgagc cacgcagcag accaaccagc
     4741 accatatcgc tgccactgtc tttgtaacgc tcagtaatct gacgacccag ttcggcgata
     4801 cgcgctttaa tctccgcttc ggggatcatt acttctacag tatgtttcat atctctaacc
     4861 atatgattta aaaacaaatc acttcgcacg ggcaggtttc agcaccgcaa caaagcgcaa
     4921 gacacgcagt ataccagtaa aactattcat tcgcggaatc tgttaataaa gctcgaattg
     4981 tgatgacgat cacacatgtt aaaacctata attaattgct actaaaatat taatgaattg
     5041 aaatggtgtc tctttatggc aattaacaat accggctcgc ggcgattact cgtcacgcta
     5101 acagcccttt ttgcagcgct ttgcgggctg tatctactca ttggcggagg ctggctggtc
     5161 gcgattggcg gctcctggta ctaccctatc gctggccttg tgatgctcgg cgtcgcctgg
     5221 atgctgtggc gcagtaaacg cgccgcgctt tggctgtacg cagccctgct gctcggcact
     5281 atgatttggg gcgtctggga agttggtttc gacttctggg cgctgactcc gcgcagcgac
     5341 attctggtct tcttcggcat ctggctgatc ctgccgtttg tctggcgtcg cctggtcatt
     5401 cctgccagcg gcgcagttgc cgcactggtg gtggcactgc tgattagcgg tggtatcctg
     5461 gcctgggccg gatttaacga tccgcaggaa atcagcggca ccttaagcgc cgataccacg
     5521 ccagcagaag ccatctcccc cgttgccgat caggactggc ctgcctatgg tcgcaatcag
     5581 gaaggtcaac gcttttcacc tctgaaacaa attaacgctg ataacgtcca caagctgaaa
     5641 gaagcctggg tgttccgtac tggcgatgtg aagcagccga acgatccagg tgaaatcacc
     5701 aatgaagtga cgccaattaa agtgggcgac accctttacc tgtgtaccgc tcaccagcgc
     5761 ctgtttgcgc tcgatgccgc cagcggcaaa gagaaatggc attacgatcc tgagctgaaa
     5821 accaacgagt ccttccagca tgtaacctgc cgtggcgtct cttatcatga agccaaagca
     5881 gaaactgctt cgccggaagt gatggcggat tgcccgcgtc gtatcattct tccggtcaat
     5941 gatggtcgtc tgattgcaat taacgctgaa aacggcaagc tgtgcgaaac cttcgccaat
     6001 aaaggcgtgc tcaatctgca aagcaatatg ccggacacca aaccgggtct gtatgaaccg
     6061 acttcgccgc cgattatcac cgataaaacc atcgtgatgg ccggttcagt caccgataac
     6121 ttctcaaccc gtgaaacatc tggcgtgatc cgtggttttg atgtcaacac cggggagctg
     6181 ctgtgggctt ttgatcctgg tgcgaaagat cctaacgcaa tcccgtctga cgaacacacc
     6241 tttaccttta actcgccaaa ctcgtgggca ccagcggcct atgacgcgaa gctggatctg
     6301 gtgtatctgc cgatgggcgt gaccacgccg gatatctggg gcggtaaccg cacaccggaa
     6361 caggaacgtt atgccagctc gattctggcg ctgaatgcca caaccgggaa actggcgtgg
     6421 agctaccaga ccgttcacca cgacctgtgg gatatggatc ttccggcaca gccgacgctg
     6481 gcggatatca ccgttaatgg tcagaaagtg ccagttattt atgctccggc gaaaaccggc
     6541 aacatttttg tgctcgatcg tcgtaatggc gaactggtgg ttccggcacc ggaaaaaccg
     6601 gttccccaag gtgctgccaa aggcgattac gtaaccccaa ctcaaccgtt ttctgaactg
     6661 agcttccgtc cgacgaaaaa tttgagcggt gcggatatgt ggggagccac catgtttgac
     6721 cagctggtgt gccgcgtgat gttccaccag atgcgctatg aaggcatttt caccccgcca
     6781 tctgaacagg gtacgctggt cttccctggt aacctgggga tgttcgaatg gggcgggatt
     6841 tccgttgatc cgaatcgtga agtggcaatt gccaacccaa tggcactgcc gtttgtttcg
     6901 aaactgatcc cacgcggtcc tggcaacccg atggagcagc cgaaagatgc caaaggcacg
     6961 ggtacggaat ccggcattca gccgcagtac ggtgtaccgt atggcgtcac gctcaatccg
     7021 ttcctctcac cgtttggtct gccgtgtaaa cagccagcat ggggttatat ctcggcgctg
     7081 gatctgaaaa ccaatgaagt ggtgtggaag aaacgtattg gtacgccgca ggacagtatg
     7141 ccattcccga tgccggtacc tgtgccgttc aatatgggta tgccgatgct gggcgggcca
     7201 atctccacgg cgggtaacgt gctgtttatc gccgctacgg cagataacta cctgcgcgct
     7261 tataacatga gcaacggtga aaaactgtgg caaggtcgtt taccagcggg tggtcaggcg
     7321 acgccaatga cctatgaagt gaatggtaag cagtatgtgg tgatctccgc aggcggtcac
     7381 ggttcatttg gtacgaagat gggcgactat attgtggctt atgcgttgcc ggatgatgtg
     7441 aagtaaggct tgctcagatt gctgacaacg tgcgatttgt tcatgccgga tgcggcgtga
     7501 acgccttatc cggcctacaa aacatttgca aattcaatac attgcgcgta ggcctgataa
     7561 gcgtagcgca tcaggcagtt ttgcgtttgt catcagtttc atgcccggag agatccgggc
     7621 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
     7681 ctccagcaaa tggcagtgcg ccatataagc atgttctttc ggtgcatcgt gattaaactt
     7741 caccagcact tcgctgacat taccttctac cttaacggta tctttccagc ctgcgcgatg
     7801 cgtcgctggc ggtttgccat tttctgacaa gatacggaac tgcgtaccgt ggatatggaa
     7861 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtatt gccctttcgc
     7921 cgccgcaaac atcggcttgt tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
     7981 gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat
     8041 catctggctg tgatccatcc cggccatcgc ctgatcgcca tatttctcca tcagcatctg
     8101 catccccatc atatcgagca ttgggtccat agaaagttgc agcttgcgta ccgtcagccc
     8161 ttccagcgaa ggtaacgcag gcaggctact taatgtgtct ggcaaagcac cagaggcact
     8221 aatggcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggcg caatcgccat
     8281 ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
     8341 cagcacttca aaacgctcgc ccatcagcac tggcagctcg ttcaccttca ccggttcagg
     8401 tagcagaccg ccgtcgctgg caatcacata aagcgggcga ttgtcgctgg tggcgaaatt
     8461 gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccagccac gcggggcagc
     8521 gtgttgtggg tagattacac cgttggtcag caacgtatcg ccaaaccaac ccacagcggc
     8581 ggtcatcaca tccagttgat aatcaatctg cccgtcagcg ttaaattttt tatcctgaac
     8641 aataaccggg acatcatcaa taccccactg ttttggcagc attaatttca ggatctcgtc
     8701 atcttcaatc accaccaaac cagccagccc catcgccacc tgacgtccgg tcttgccatg
     8761 ttgatgtgga tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg
     8821 cttgccacct ggcggaataa ttccctgcgg gccgccgtcc acttcacccg gtacttccag
     8881 cccgtgccag tgcaacgtcg tctcttccgt cagttggttg tagatatcaa ccgttaccgc
     8941 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
     9001 tgcggttttc ccgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg
     9061 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaatactgc
     9121 gcggctccac agcggcaaag ccgaagccac gcccagcgcg acggaatatt ttaagaaatc
     9181 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
     9241 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
     9301 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
     9361 aagacgtttt tcagaacagt gttattcggc agcctgatgg ccgtctgcgc aaacagttac
     9421 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtttttctg
     9481 aagaacgatt gtggttacca gaacttacct aacgggcaaa ttcgtcgcgc actggtcttt
     9541 tttgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagccctc
     9601 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaagtgcaaa
     9661 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
     9721 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
     9781 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
     9841 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
     9901 gatcaccagg atctgatcat ttttgagaac gccgcatttg gtcgcgtaat ggcgctggat
     9961 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    10021 ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    10081 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    10141 gcgggtgtcg tgtcgttctg ccgtcagtat ctacccaacc ataacgccgg tagctacgac
    10201 gatccgcgct ttaaactggt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    10261 acctttgatg tcattatttc cgactgcacc gatcctatcg gtcccggcga aagtctgttc
    10321 acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    10381 caaaacggcg tctgcttttt acagcaggaa gaggccatcg acagccatcg caaactcagc
    10441 cattacttca gcgatgttgg cttttatcag gcggcgatcc cgacctatta cggcggcatc
    10501 atgacctttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    10561 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc agtccatacg
    10621 gcagcttttg ccttacccca gtatctgcaa gatgcactgg cttcacagcc gtcctaagga
    10681 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    10741 agtttttgta tttacgatat ctgctacgcc aaaactaccg aagagcgcga cggttatatt
    10801 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    10861 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgagcc acaaggtgcc
    10921 agcgtcacta ttctggtgag tgaagaaccg gtcgacccga aacttatcga caaaacagaa
    10981 caccccggcc cactgccaga aacggtcgtt gcccatctcg ataaaagtca tatttgcgta
    11041 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    11101 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    11161 gagtccgata ttgtgactat tgattatcgc gtgcgcggtt ttacccgcga cattaacggt
    11221 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    11281 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    11341 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    11401 accgacagcg agcgccagga aattaccact gcgctgtgga aagaaatgcg cgagatttat
    11461 tacgggcgca atatgcccgc tgtttaacgt ctcaggcgga gcacggaggc tccgcctgat
    11521 ttacttactt ctgctgcacg aaattacggt aagccgccac cacctgaaga aaatcctcaa
    11581 cgccgcacag cgacaagctc tcttcatcgt agtagttcat cccctcttcc atttcatcgc
    11641 cagcgaattc cagttgattg gcgcgaacca tcacctcttc accatccatc catagcgtgt
    11701 attcatgccc tgcccgttgc caggaacgtt cgctaccttt caatgcgtgc gccgcttgtt
    11761 ctacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    11821 cttcatgccc catggacatg cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    11881 aatccatagt gtgttcctct gctcctggca atgcgccgaa atcgcattgc gccattgatg
    11941 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatggtttag aaataaaaaa
    12001 tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt
    12061 attaaaccgc agtctggaaa atcaccccgt ccgctttttc ggtgtactga gaaagctggt
    12121 cgaagttcag ataacggtaa gtatcaacgg ctgttttatc tacctgcgcc acgtaggtct
    12181 ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacagcagcc agttccgcag
    12241 aagccaggaa gacattcgcg ccagtaccca aacggttcgg gaagttacgg gtagaggtgg
    12301 aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagccag
    12361 ggatctcgat acgagcacca ctcttaccga agacgctgta gtagccttct tcggtcagct
    12421 gcgcagcgtc catacgcgtt ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt
    12481 gcgcatccag cagtttacca gcagcacgga agtgaccgat gttagtcatg caagaaccga
    12541 taaatacttc gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcgtccgggt
    12601 cattcggtgc acagaggatt ggctctttga tatccgccag atcgatgtcg atcactgccg
    12661 cgtattccgc atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc
    12721 cctgaatacg acgctccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca
    12781 gcaggacgat gttagagttc aggtattcaa tgatcggttc tttgttcagc ttgatggtac
    12841 aaccggcagc agaacgctcg gcagacgcat cggtcagctc aaatgcctgc tcaactttca
    12901 gatccggcag accttcaatt tccagaatgc ggccagagaa gatgtttttc ttacctttct
    12961 tctcaacggt cagcagacct tgtttgattg catacagcgg gatcgcgtgt accagatcgc
    13021 gcagggtgat acccggctgc attttgcctt tgaagcgcac cagaacggat tctggcatat
    13081 ccagcggcat tacgcctgtt gcggctgcaa atgccaccag accagaaccc gccgggaaag
    13141 agataccgat cgggaaacgg gtatgggagt caccaccggt accgacagta tccggcagca
    13201 gcatacggtt cagccaggag tggattacgc cgtcacccgg acgcagcgac acaccgccac
    13261 ggttcataat gaagtccggc agcgtgtggt gcgtgttaac gtcaactggt ttcggatacg
    13321 ccgcggtgtg gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt
    13381 ctttcagttc atcacgggtc atcgggccgg tggtgtcctg ggaacctaca gaagtcattt
    13441 tcggttcaca gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt
    13501 tctgcgccag cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat
    13561 cgctgtgcgg caggccaagt gcttcacgcg ctttggtggt caggccacgc ccaataatca
    13621 gcggaatacg gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg
    13681 tcgccagcag ttcgccggtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa
    13741 ttacgtcgcc catgttcagg ttagagacgt cgacttcgat tggcagcgca cccgcgtctt
    13801 ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt
    13861 tgttcggcac atgtggaata tcatcgccca taaaccacag aacggagtta gtggcggatt
    13921 tacgcgatga ccccgtaccc acaacgtcac cgacgtacgc cagtgggaaa cctttctgtt
    13981 gcagagcttc gatttgctta atcggaccaa caacgcctgg ctgatctggc tcaatacctt
    14041 cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat
    14101 ccggtgccgg agagaggtca tcggtgttgg tttcgcctgt gactttgaag acggtaacgg
    14161 tcagtttttc agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca
    14221 ttacctgctt cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat
    14281 cgaacatcag cagcgtgtga gaaagtgctt tggcagcgat cggtgccagt ttggcatcat
    14341 ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga
    14401 tggctttttc cggagtcagc agaggggatt tggcttcgcc tttagcgata gcagccagga
    14461 agcctgcttt tacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat
    14521 ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agcgcggcca
    14581 tttggtttgc atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct
    14641 tacggtattc ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga
    14701 ttctcttcac gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg
    14761 cagaatagca ggattttgac gatgtgttaa tctgtttaca aaaaagcaac ataaaaaagt
    14821 ggctgaatcg ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg
    14881 catggtgata atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc
    14941 tctttatact cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct
    15001 tgagatccga attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg
    15061 cagtgccggg ctttgtgccg cctctaccgg gctatatata aaaagccgca cagtggaagc
    15121 gcctgtggaa ccgcaatcga cacaactggc tgcgcctgac atctccgcag ttacgcttcc
    15181 tgcaacggtt tccgtgcctc ctgtaacacc tgccgtcgtc aaatccgcat tcagcactgc
    15241 gcaaatagat caatgggttg cacctgtcgc gctatacccc gactctctgc tttcacaagt
    15301 gttaatggca tcaacctatc cggcaaacgt tgctcaagca gtgcaatggt cgcacgataa
    15361 tccacttaaa caaggcgatg ctgctattca ggcggtatct gaccagccct gggacgccag
    15421 cgtcaaatca ctggtggcct ttccacagtt gatggcattg atgggcgaaa acccgcaatg
    15481 ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag gaggtgatgg actcggtaca
    15541 gcgattgcgg caactggcgc aacaaaccgg ctcgctgaag tcatcaactg aacagaaagt
    15601 tattaccaca acgaagaaag ctgtaccggt aaaacagaca gtcacggctc ccgtcatacc
    15661 atccaatacc gtttcaactg ccaaccctgt cattacagag cctgcaacaa ccgtcatttc
    15721 cattgaaccc accaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta
    15781 cgggaactgg gccaatactg cgtatccgcc ggtttatctg ccgccgccag ccggagaacc
    15841 gtttgttgac agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc
    15901 gctattcagc agcatcgact gggatgacga cgatcatgac catcatcatc atgacgatga
    15961 tgattatcac catcacgatg gcggtcatcg tgatggtaat ggctggcaac acaatggcga
    16021 caacatcaat atcgacgtca acaatttcaa ccgtatcacc ggtgaacatc ttactgataa
    16081 gaatatggca tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga
    16141 tatggcaaag cggtttcatc aaaccgatgt caacggcgga atgagcgcca cgcagctacc
    16201 tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca
    16261 cagcgcacca gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga
    16321 agctgaaaac tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac
    16381 ccagcaacaa aaggacgccg ctcgtcagcg ttatcagtcg gcctctcctg agcagcgcca
    16441 ggcagtccgc gagaaaatgc agactaaccc gcagatccag cagcgaagag aggcagcgcg
    16501 tgagcgcatt cagtctgcaa ctcccgaaca gcgccaggtg tttaaggaaa aagtacagca
    16561 gcgcccactg aatcaacagc aacgtgataa cgcccgccag cgtgttcaat cagcatcacc
    16621 tgagcaacgt caggtttttc gggagaaagt tcaggagagc cgcccgcaac gtctaaacga
    16681 cagtgaccat actgtcaggc tgaataacga gcaacggtca gcagtacgcg aacgtctctc
    16741 tgagcgcgga gcaaggcgac tggaaaggta aattacagac gtaaaaaaag cggcgtggtt
    16801 agccgctttt ttaattgcca gatgttccgg caaacgaaaa attacttctt cttcgctttc
    16861 gggttcggca ggtcggtaat gctaccttcg aacacttctg ccgccaggcc cacagactcg
    16921 tgcagagtcg ggtgcgcatg gatggtcagt gcgatgtctt cagcgtcaca acccatttcg
    16981 attgccaggc cgatttcacc cagcaactcg ccgccgttag tgccgacaat cgcaccaccg
    17041 atcacacggt gagattcttt gtcgaaaatc agcttggtca taccgtctgc gcagtcggaa
    17101 gcgatagcac gaccagaagc agcccacggg aaggtggcgg tttcgtagct gatgcctttc
    17161 tctttcgctt ctttctcagt cagacctacc catgcaactt ctggttcggt ataggcgatg
    17221 gacgggataa ctttcggatc gaagtaatgt tttttaccgg cgataacttc agcggcaacg
    17281 tgaccttcgt gaacaccttt gtgtgccagc attggctgac cgacgatatc accgatagca
    17341 aagatgtgcg gtacgttggt acgcagctgt ttgtcaacgc ggatgaaacc acggtcgtca
    17401 acttccacgc cagctttgcc tgcgtcgagg tttttaccgt tcggcacacg accaatcgct
    17461 accagcacgg cgtcgtaacg ctgcggttca gcgggtgctt ttttgccttc catcgtcaca
    17521 taaatgccgt cttctttcgc ttcaacggcg gtaactttgg tttccagcat caggttgaat
    17581 ttcttgctga tacgcttggt aaagacttta acgatgtctt tatcagctgc cgggatgacc
    17641 tggtcgaaca tttcaaccac gtcaatctgt gaacccagcg cgtggtatac ggtgcccatt
    17701 tccagaccga tgataccgcc acccattacc agcaggcgtt ctggtacttc tttcagttcc
    17761 agcgcgtcag tggagtccca gatacgcgga tcttcatgcg gaataaacgg cagttgaatc
    17821 gggcgagaac ccgctgcaat gatcgcgttg tcgaagttga tcacggtttt accgttctca
    17881 ccttcaactt ccagggtgtt agccccggta aatttaccca gaccgttgac cactttgact
    17941 ttgcggcctt tcgccatacc agccagacca ccggtcagct gattgatcac tttctctttc
    18001 caggtacgaa tcttgtcgat atcggttttc ggttcgccaa agacgatacc gtgttcagcc
    18061 agcgctttgg cttcttcgat aacttttgct acgtgcagca gtgctttaga agggatacag
    18121 ccgacgttca ggcaaacacc gccaagggtg ttgtagcgtt ctacgattac ggtttccaga
    18181 cctaaatcag cgcaacggaa ggcagcggag taacctgcgg ggcctgcccc aagtaccacg
    18241 acctgagttt tgatttcagt actcatcatg acctctatat atttatctcc ggcggtcata
    18301 cccgtcgtct ttcaggccgc agcggcgtta gcttcacgcc ctcaccccag tcacttactt
    18361 atgtaagctc ctggggattc cggcgcttgc taccttgctg cacccagaaa tccatagggt
    18421 atgtgaagta ataccctaac caccaccggg tcgttctatc cgtcggtatt ttacaaaatt
    18481 gttaacaatt tttaaacaac aaacggcaac cgatttgtct attcgctaat aacctcaata
    18541 cattcatgag attaccagaa aaaagccggc cgttgggccg gctcttttac ttacatcacc
    18601 agacggcgaa tgtcagacag cgtgttgtta atgatggtaa tgaaacgggc accatcagca
    18661 ccgtcgatca cgcggtggtc gaaggagaga gaaatcggca gcatcagacg cggcacgaac
    18721 tctttaccat tccacaccgg ctccatcgcg gacttggaaa cgccgaggat agccacttcc
    18781 ggcgcgttca caatcggcgc gaagtgggta gtacccaggc cgccgatgct ggagatggtg
    18841 aagcaaccgc cctgcatttc gcccgcagtc agcttaccgt cacgcgcttt cttagaaata
    18901 gtcatcagct cgcgagacag ctcgatgatg cctttcttgt tgacgtcttt gaataccgga
    18961 acaaccagac cgttcggggt atccaccgcc acaccgatgt tgatgtattt cttcagggtc
    19021 agacgctgac cgtcttccga cagcgaactg ttgaagcgag gcatctgctc aagagctgca
    19081 gcaacggctt tcatgatgaa gacaaccggg gtgatcttca catccagctt acgtttcgcc
    19141 gcttcttcgt tctgctgttt acggaacgct tccagctcgg tgatatcggt tttgtcgaag
    19201 tgggtaacat gcgggatcat cacccagtta cggctcaggt tcgcaccaga gattttctgg
    19261 atacggccca gttccacttc ttcgatttca ccaaacttgc tgaagtccac cttcggccac
    19321 ggcagcatgc ccgggatacc gccgccagtc gccgccggag ccgcttctgc acgtttgata
    19381 gcttctttca cgtaagcctg aacgtcttcg cgcaggatac gacctttacg gccagtgccc
    19441 ttcactttcg ccaggttaac gccaaattcg cgtgccagac ggcggatcag cggagtcgcg
    19501 tgaacgtaag cgtcgttttc agcaaattca gatttgcctt cggctttcgc agccggagct
    19561 gctgctggag cttcagcttt tgctgccgga gccggcgctg ccgcttcctg tttcgcagga
    19621 gctgccgcag gcgctgcgcc ttcaacttcg aagatcataa tcagcgagcc agttttcact
    19681 ttatcgccaa cgttgacttt cagttccttc acgacgcctg caaacggagc cggaacttcc
    19741 atagacgctt tatcgccttc tacggtgatc agtgactgtt cagcggcaac tttgtcgccc
    19801 actttcacca tcacttcggt cacttcaact tcgtcaccgc cgatatccgg aacgttaacg
    19861 tctttcacgc cagccgctgg tgcagaggcc gctgccggag ccgcttcctg tttagccgcc
    19921 ggagctgccg cgcctgcttc acccgcgact tcgaagacca taatcagcga gccggtagac
    19981 actttgtcac ccacgttcac tttgatctct ttcacggtgc cagcaaacgg agccggaact
    20041 tccatagaag ctttatcgcc ttctacggtg atcagcgact gttcagcttc aactttatcg
    20101 ccaactttca ccaggatttc ggtcacttca acttcgtcgc tgccgatatc cggaacgtta
    20161 acgtcttttg ccgctgcagc cgctggagct gctgccggag cggcttcttt cttctcttct
    20221 gcctgagcag gtgcagcgtc tgctgcaccg tcggcggaat cgaaaatcat aatcagtgcg
    20281 ccggtctggg ttttatcgcc aacagagact ttgatctctt taacgatacc cgcctgcgga
    20341 gacggaactt ccatagaggc tttgtcgcct tctacggtga tcagcgactg ttcggcttca
    20401 actttgtcgc ccactttgac caggatctcg gtgatttcaa cttcatcagc cccgatgtcc
    20461 ggtactttga tttcgatagc cattattctt ttacctctta cgccagacgc gggttaactt
    20521 tatctgcatc gatgttgaat ttggcgattg cgtcagcaac cactttctta tcgatttcgc
    20581 cacgtttagc cagttcgccc agcgccgcaa ccacgacata agaagcatca acctcgaagt
    20641 ggtgacgcag gttctcacgg ctgtcggaac gaccgaagcc atcagtaccc agtacgcggt
    20701 agtcgtcagc cggtacgtaa gtacggactt gctcagcgaa cagtttcata tagtcggtag
    20761 atgccactgc cggagcgtcg ttcatcacct gagcgatata cggaacgcgc ggagtttcca
    20821 gcgggtgcag catgttccag cgttcacaat cctgaccatc acgcgccagt tcggtgaagg
    20881 aagtcacgct ataaacgtca gaacctacgc cgtaatcttt cgccaggatc tctgctgctt
    20941 cacggacgtg acgcaggata gaaccggagc ccagcagctg aactttacct ttgctacctt
    21001 caatggtttc gagtttgtag atacctttac ggataccttc ctcagcacct tccggcattg
    21061 ttggcatgtg gtagttttcg ttcagcgtag tgatgtagta gtaaacgttc tcttgtttct
    21121 caccgtacat acgctccaga ccgtcatgca taatgacagc aacttcgtaa gcgtaagccg
    21181 ggtcgtaaga gatacagttc gggatagtca gcgactgaat gtggctgtga ccatcttcgt
    21241 gctgcagacc ttcgccgttc agggtggtac gaccggaagt accgccgatc aggaagccac
    21301 gcgcttgctg gtcaccagcc gcccagcaca gatcgccgat acgctggaag ccgaacatcg
    21361 agtaatagat gtagaacgga atcatcggca gattgttggt gctgtaagag gtcgccgctg
    21421 ccagccagga acacccagcg cccagctcgt tgatcccttc ctgcagaatc tgacctttct
    21481 cgtcttcttt atagtaagca acctgctcgc ggtcctgcgg ggtgtactgc tgaccgttcg
    21541 ggctgtaaat accaatctga cggaacagac cttccatacc gaaagtacgc gcttcgtcgg
    21601 cgatgatcgg taccagacga tctttgatcg acttgttctt cagcattacg ttcagggcac
    21661 gaacgaaagc gatagtggta gagatctctt tgctctgctc ttccagcagc gcgccgaagt
    21721 cttgcaggct cggcagctca agcttctcgg tgaagttcgg ctgacggctt ggcagataac
    21781 cgtgcagttt ctgacgctga gcgtgcagat aggtatgctc ttcagaacct tccgggaagg
    21841 tgatgtacgg caatttttcg atatcggcat cagacaccgg cacattgaaa cggtcgcgga
    21901 tgtgacgcac gccgtccatg ttcattttct taacctggtg cgcgatgttt ttaccttcag
    21961 ccgcgtcgcc catgccgtaa cctttaatgg tatgagcaag gattaccgtc gctttgcctt
    22021 tggtttcctg cgctttctta aatgcagcgt agattttctt cggatcgtga ccaccacggt
    22081 tcagtgccca gatctgttcg tcagtccagt ctgcaaccag tgctgcggtt tcaggatatt
    22141 taccgaagaa gtgttcacga acgtacgcac catctttcga tttgaaggtc tggtagtcgc
    22201 cgtcaacggt ttcgttcatc agctggatca gtttaccgct ggtatcttta cgcagcagtt
    22261 catcccaacg gctaccccac atcactttga tcacgttcca gccagcacct tcgaagatgc
    22321 cttccagttc gttgatgatc ttgccgttac cggtgaccgg gccgtcaaga cgctgcaggt
    22381 tacagttgat aacgaaaacc aggttatcca gtttttcacg ggtagcgatg gtgatcgcac
    22441 ctttggattc cggttcgtcc atttcaccgt cgccgaggaa cgcgtaaacg gtttgtttag
    22501 aggtatcttt caggccacgg tgttccagat atttcaggaa tttagcctgg taaatagcac
    22561 caatcggccc cagacccata gatacggtcg ggaactgcca gaattccggc atcagtttcg
    22621 ggtgcggata ggaagagagg ccattgccgt gaacttcctg acggaagtta tccagctgct
    22681 cctgagtcag acgaccttcc aggaaagcac gagcgtaaac gcccggggag atgtggccct
    22741 ggaagtaaac caggtcgccg ccatcctgct cgttgcgtgc acggaagaag tggttaaagc
    22801 acacatcata aatggttgcg gaagactgga aggacgccat gtggccgccc agttcgaggt
    22861 cttttttcga cgcacgcaga accgtcatga tggcgttcca gcggatagct gaacgaatac
    22921 ggcgttccag ttccagatta cccggatact ccggttgttc ttcaacgggg atggtgttga
    22981 tgtagttgct gataccggtg cctgcggcta cgttgacacc gcctttgcgg gcttcagcaa
    23041 gcagttggtc gatcagatac tgagcacgct caacaccttc ttcacggatg accgattcga
    23101 tcgcctggag ccagtcgcga gtttcgatcg gatccacgtc atttgggaaa cgttctgaca
    23161 tgggttattc cttatctatc taataacgtt gagttttctg gaacctgtcc cattgaactc
    23221 tcgccggaaa gctcaataag acaggttcta cgtttagttg ccgcgctttt atatgcgctt
    23281 gatttacaac atcttctgga taatttttac cagaaaaatc actaattctt tcgttgctcc
    23341 agacgacgta gagaacgctc acggcggctc tcttcacggc ttctgtcgag caaaatttct
    23401 tcgataaagg ccagatggcg atgcgatgct tcgcgcgctt cttccggctt accggccata
    23461 atcgcttcaa atatgcgggt gcggtgacta ctcaccagcg gcagcatctc gcgacgcgaa
    23521 tagagcaatt cgaagttctg gcggacattc tgggccaaca tcggctccat acaccttagc
    23581 agatgaagca gaaccacatt gtgggccgct tcggtgacgg caatctgata ctggagtacg
    23641 gcatttgatt ccgcgtccag atcgccagac tgctgcgcca gctctatggc atggtggagt
    23701 tcacggatgc gttccttgtc ttcatcggta ctacgcagcg cggcgtaata agcggcgata
    23761 ccttccaggg cgtgtcgtgt ttcgagcaag tcatactgtg attcaggatg gtcggagagc
    23821 agctccacca gcggatcgct gaagctttgc cataggctgc tctggacaaa agtgccgcca
    23881 ccctggcgac gaagcaacaa gcccttcgct tcgagacgtt gaatcgcctc acgcaaggag
    23941 ggacgggaga cgtcgaactg ttttgccagt tcgcgttccg gtgggagttt ttcgcccggg
    24001 cggagagtgc cttcgaggat caaaaactcc agttgctgct caatcacatc ggagagtttt
    24061 ggttggcgga ttttgctgta ggccatgagt tcctgtctta agccacttgc cgaagtcaat
    24121 tggtcttacc aatttcatgt ctgtgacgct aaagtaacaa agtattcacc ttatgtccat
    24181 acaggttttg attgaaatca tgaaactgtg cacattttaa caacttgaca tatataacgt
    24241 ttcaaagttg taactatgca caaatgttaa atttacccat caaggggtga gattaacaga
    24301 attgaaacga aaaaatatgt cctctgaacc gattcaccaa gcaacaaaac gcataacaat
    24361 tcaattatgt gaattaata
//
LOCUS       DAZKGF010000071        24355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZKGF010000071 DAZKGF010000000
VERSION     DAZKGF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.071"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(130..1011)
                     /gene="prmA"
                     /locus_tag="ACHOQV_002607"
     CDS             complement(130..1011)
                     /gene="prmA"
                     /locus_tag="ACHOQV_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP9630966.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1023..2474)
                     /gene="panF"
                     /locus_tag="ACHOQV_002608"
     CDS             complement(1023..2474)
                     /gene="panF"
                     /locus_tag="ACHOQV_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFP9630967.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFIGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTIVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRTVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2464..2706)
                     /gene="yhdT"
                     /locus_tag="ACHOQV_002609"
     CDS             complement(2464..2706)
                     /gene="yhdT"
                     /locus_tag="ACHOQV_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFP9630968.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2815..4164)
                     /gene="accC"
                     /locus_tag="ACHOQV_002610"
     CDS             complement(2815..4164)
                     /gene="accC"
                     /locus_tag="ACHOQV_002610"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP9630969.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4175..4645)
                     /gene="accB"
                     /locus_tag="ACHOQV_002611"
     CDS             complement(4175..4645)
                     /gene="accB"
                     /locus_tag="ACHOQV_002611"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP9630970.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5050..5437)
                     /locus_tag="ACHOQV_002612"
                     /pseudo
     CDS             complement(5050..5437)
                     /locus_tag="ACHOQV_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240072.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            complement(5623..6597)
                     /gene="yhdH"
                     /locus_tag="ACHOQV_002613"
     CDS             complement(5623..6597)
                     /gene="yhdH"
                     /locus_tag="ACHOQV_002613"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFP9630971.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEVVVTGASGGVG
                     STAVTLLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            6749..8689
                     /gene="csrD"
                     /locus_tag="ACHOQV_002614"
     CDS             6749..8689
                     /gene="csrD"
                     /locus_tag="ACHOQV_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFP9630972.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8994..10037
                     /gene="mreB"
                     /locus_tag="ACHOQV_002615"
     CDS             8994..10037
                     /gene="mreB"
                     /locus_tag="ACHOQV_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFP9630973.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10103..11206
                     /gene="mreC"
                     /locus_tag="ACHOQV_002616"
     CDS             10103..11206
                     /gene="mreC"
                     /locus_tag="ACHOQV_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP9630974.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11206..11694
                     /gene="mreD"
                     /locus_tag="ACHOQV_002617"
     CDS             11206..11694
                     /gene="mreD"
                     /locus_tag="ACHOQV_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP9630975.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11703..12296
                     /gene="yhdE"
                     /locus_tag="ACHOQV_002618"
     CDS             11703..12296
                     /gene="yhdE"
                     /locus_tag="ACHOQV_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFP9630976.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12286..13755
                     /gene="rng"
                     /locus_tag="ACHOQV_002619"
     CDS             12286..13755
                     /gene="rng"
                     /locus_tag="ACHOQV_002619"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFP9630977.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13823..17623
                     /gene="yhdP"
                     /locus_tag="ACHOQV_002620"
     CDS             13823..17623
                     /gene="yhdP"
                     /locus_tag="ACHOQV_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFP9630978.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGQVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTH
                     GDSTNSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17779..19224
                     /gene="tldD"
                     /locus_tag="ACHOQV_002621"
     CDS             17779..19224
                     /gene="tldD"
                     /locus_tag="ACHOQV_002621"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFP9630979.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19352..20281)
                     /gene="aaeR"
                     /locus_tag="ACHOQV_002622"
     CDS             complement(19352..20281)
                     /gene="aaeR"
                     /locus_tag="ACHOQV_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFP9630980.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20464..20667
                     /gene="aaeX"
                     /locus_tag="ACHOQV_002623"
     CDS             20464..20667
                     /gene="aaeX"
                     /locus_tag="ACHOQV_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFP9630981.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20675..21607
                     /gene="aaeA"
                     /locus_tag="ACHOQV_002624"
     CDS             20675..21607
                     /gene="aaeA"
                     /locus_tag="ACHOQV_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFP9630982.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21613..23580
                     /gene="aaeB"
                     /locus_tag="ACHOQV_002625"
     CDS             21613..23580
                     /gene="aaeB"
                     /locus_tag="ACHOQV_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFP9630983.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23672..23944
                     /gene="yhcO"
                     /locus_tag="ACHOQV_002626"
     CDS             23672..23944
                     /gene="yhcO"
                     /locus_tag="ACHOQV_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFP9630984.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(23999..24262)
                     /gene="yhcN"
                     /locus_tag="ACHOQV_002627"
     CDS             complement(23999..24262)
                     /gene="yhcN"
                     /locus_tag="ACHOQV_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFP9630985.1"
                     /translation="MKIKTTVTALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5785 a         6145 c         6590 g         5835 t
ORIGIN      
        1 taagttctca atttttcttt atgaatgaaa attttggcac gcaaatttgc gtaaataatc
       61 ggcaaattaa cgtgcctttt gtgacatcgc tcgcacttcg ctctgcgtca ccctacggcg
      121 atgcgaaggt taattcttac gaccggtaat gcggcaccac tcttcttttt ccacgaccgg
      181 gtccagagcg aagctgtcgg cataagcttc gcaaacgctc tctgcctggc ttgccagaat
      241 accggaaagg cccagcaaac cgcctgaaac tggcaggacg ctgattaacg gtgccagttc
      301 acgtaatggg cctgcaagga tgttagcgac caccacgtcg gctttcattt cttctggctg
      361 atctttcggt aagtagagtt ccagacggtc agagacgcca ttacgttccg cgttgtcgcg
      421 gctggcctga atcgcctgcg gatcaatatc aataccaatg gcttttgctg cacccagttt
      481 cagcgccgcg atagccagaa tgccggaacc gcagccaaag tcgatgactg ttttaccggt
      541 taaatcgagg ctgtcgagcc actgcaggca cagagaggta gttggatggg tacccgtacc
      601 aaacgccagc cctggatcta acatcacgtt gacggcgttt tcgtccggca catcacgcca
      661 gcttgggcag atccacagcc gttcaccaaa gcgcatcggg tggaaattat ccatccattc
      721 gcgctcccag tctttatctt ccagttgttc gattttatgc gcgaagcccg cgccgagcag
      781 cggatggttt tccagaatcg ccaccacgtc gttcatatcg gtttcagcgt cgaacagacc
      841 aatcacatcg gtgtcgcccc acaggcgcgt ttcgcccggc agcggctcaa acacaggcgt
      901 atcgtgggta tcctgaaaag tgatagaaac ggcacccgct tccatcagcg catcgctaag
      961 atcttccgcg ttcgcgccgg tggtgttcag tttcagttgg atccaaggca tggcaaaact
     1021 ctttatttat cagtagtcaa aacggtagct tgcgggacgg atgtaccgaa acggtttccg
     1081 accaggaaag ccagcaaact tagtagtaac gagggcacga tagggtggaa gcccaggtac
     1141 tgaatattca gcgtcgcgag tacggcatac agtacgccgc caacaatcat cgcacttagc
     1201 gcgcctttgg cgttggcgcg ttcccagtaa agacccagta ccagcggcca caggaaaacg
     1261 gcttccagcc caccgaaggc cagcaaattc agccagatga tcatttctgg cggcttccag
     1321 gcggcaagca gcagcaacgc accgagaact aacgtaatta ccgccgacat ccgtttcaga
     1381 cgcgtctcgt tttgcatttg atccggacgg atattcagat agagatcttt aatgatcgta
     1441 gcggaacttt gcagcagttg ggcgttaatt gtcgacatga tcgcagccat aggtgcagcc
     1501 aggaagatcc cggcagcaaa cggtggcaac acttttacca ttaacgtcgg gatcaccagg
     1561 tccggtacgg tgagatcggg gatcaccgtc cgacctaacg ctccggccag gtgcataccg
     1621 aacatcagga ttgcgaccac aatcgtaccg atgatgatcc cccgatgtac ggctttgctg
     1681 tctttataag agatacagcg caccgcagta tgcggcaggc caatcacgcc aaagcacacc
     1741 agtacccaga acgacgtcat aaaggcaggc gacagaatat cgtcagcgcc ttgtggcgta
     1801 accagttgcg gatcgatggt ttgcaacgtc tctactgcgt tgcttaagcc acctgcggca
     1861 tgtaccacgc caataagcag cacaatggtg ccaatcagca tcaccagccc ttgcatagtg
     1921 tcgttcagca cgctggcgcg aaagccacca aaggcggtat acaacgcaat gctgataccg
     1981 aaaatcagca gcccggtttc ataaggaata cccgccgcgg tttccagcag gcgcgcgccg
     2041 ccgataaact gcacggtcat cgcgccaata aacgcgacca gcaaactcaa actcgccagc
     2101 cacaccaaaa gacgactctg gtagcgggca aacagcatgt cgttcagtgt caccgcattg
     2161 tagcggcgcg caagaattgc gaacttcttg ccgagaatac cgagcgaaag ccagactgca
     2221 ggaagctgaa tcatcgccag caatacccag cccaggccgt atttataagc agctccaggc
     2281 ccgccgataa acgaactggc actgatatag gtcgcggtga gcgtcatcgc cagcacaata
     2341 ccgcccatag agcggctgcc gaggaaatac tcattaagga aggtgccagt gctccgttta
     2401 cgcatcgcat aaacagagat accgaacacc accaccagat aggcgaccag cggtagaatt
     2461 acttcaagct gcatcgtcat cctccagtgg gatatcgcga tagataaatt tcaccatcgc
     2521 ccagcacagt ccaataaaca gcagcggcgt caggatgcag gccatctcaa accagcgcgg
     2581 aaaaccggta aaaccggggg caacgccagg taagtaagcg gctactaacc ataccgccag
     2641 atacaaaagg gtcagcccca gcgcccagcg cgcctcttta tgggcctgaa caaaacgagt
     2701 gtccattttt tgtccctgat gggtgaagaa agcggggatt gtaccttatg ggggttgtcg
     2761 atccccagta ataaaaaagg ccggaaaatc cggccttttg acgcttttgc agtcttattt
     2821 ttcctgaaga ccgagttttt tctccagata gtggatgtta gtgccaccat gctggaagtt
     2881 ctcgtcattc atgatgcgga tctgcagatc aacgttggtt ttgataccgt cgatgatcag
     2941 ctcctgcagc gcattcttca tgcgggcaat cgccacgtca cggttttcac cgtagcaaat
     3001 cagcttaccg atcattgagt catagtacgg cggtacggtg tagcccgcgt agatatgaga
     3061 ctcccaacgc acgccaaaac cgcctggtgc gtggaaacgg gtgattttgc ccgggcttgg
     3121 caggaaggtg ttcggatctt cggcgttgat acggcattcc accgcatggc cgcgaacgtg
     3181 aacttcttct tgcttgatcg acagcggttg accggcagcg atacgcagct gttctttgat
     3241 caggtcaacg ccggtgatca tttcagtaac cgggtgttct acctgaatac gggtgttcat
     3301 ttcgatgaaa tagaactcgc cgttttcgaa caggaactcg aaagtacccg caccgcgata
     3361 gccgatatca acacatgctt tagcgcaacg ttcgccgatg tagcgacgca gttccggggt
     3421 aatgcccggt gctggtgctt cttcgaccac tttctggtgg cggcgctgca tggagcagtc
     3481 acgttccgcc agatagatag cgttgccctg accgtcagcc agtacctgaa tctcgacgtg
     3541 gcgaggattt tccaggtatt tctccatgta aaccatatcg ttgctgaaag cagctttcgc
     3601 ttccgcacgg gtcatggaga tggattgtgc cagttcagcg tcgccgcgca ctacgcgcat
     3661 accgcgaccg ccgccgccgc cggaggcttt gataattacc ggataaccaa tgcgtttagc
     3721 aatggcgcgg tttttatcca tatcgtcgcc cagcgggccg tcagaacccg gtacgcaagg
     3781 aacgcccgct tttttcatcg ccgcgattgc ggatactttg tcgcccatca ggcgaatggt
     3841 ttctgctttc gggccgatga agataaagcc ggagcgttca acctgctcgg caaagttggc
     3901 gttctcggag aggaagccgt aacccggatg gattgctact gcgccggtga tttcagcggc
     3961 gctgatgatt gccgggatgt tcagataact ttttactgac ggagcagggc cgatacagac
     4021 cgtttcatct gccagtaata cgtgttttag atcgcgatcc gcgctggagt gcacagcgac
     4081 agtcttgatg cccagttctt tacaggcacg aagaatacgc aatgcaatct cgccgcggtt
     4141 ggcaataaca attttatcca gcatgttcgc ctcgttactc gatgacgacc agcggctcgt
     4201 caaattctac cggttgtcca ctttcgacca gaattgcttt cacggtaccg gatttgtccg
     4261 cttcgatctg gttcatcatt ttcatggctt caacgatgca cagggtatcg cccacgttga
     4321 ctttctgacc cacttcgatg aacgcttttg cgtccgggct tggggtgcgg tagaaagtac
     4381 caaccatcgg ggaacgtacg atgtgaccac tgatttccgc tgctgctggc gcttccatgg
     4441 aaggaacggt cgccggagcg gctgcgttag attgagctgg ctgctgcatc attggtgcag
     4501 cgtaagcttg ttgcatcaca gggaaacttg cggcaggagc tgcacggcta atgcgtactg
     4561 actcttcgcc ttcagaaatt tccagttcgg agatgcctga ttcttcaacc agctcgatca
     4621 gttttttaat cttacgaata tccatgagtg ggttccgtac tctttgttta gtgtgattgt
     4681 gacaggcgtt tcaccgccgt ctgtaaagcg tatgccccga cagctgtatg catagcgata
     4741 aattccagca ggccgggacg ccggtctatt ttgccccaga gtgtcaatgt tagacttgac
     4801 ggacattgtg cagcgatctg ctgctatcct ccggcaaaaa acaaaatata ccttcgttgc
     4861 gccaatgtca ccttttccgc agcgcaaaaa ctgcgtcagg gaggacgagg tcgcacatta
     4921 taacgatttc gtagcaattg gcagctaaat actggtctta tcagggaaga taatcaacag
     4981 ctaacatgta aataaccttc aacaccgtgt aatttgcaac aagccgcaca attcacgaaa
     5041 ttagcgccat catcgacgga acttcttata acgtagggtg aaaagctgta aagccagccc
     5101 tgcgaagatg aggggctgca atgagataat cttcacagac cacaaataat gtagggatgc
     5161 caggatcgcg acaagataga cgacattacg cagcggttgc tgaggattac gcaattttca
     5221 ctgcatcgtc cggaaagatg tgaatgctaa agcacgcaaa attaaccgac tgacaatgtt
     5281 ccgcattcaa taggtttaag tggttaattt gcaatcaaat aaaatcagat tattcccccc
     5341 atggtaccag aaatcagtaa cagattaagc gtagcgtcgc caaggcaaaa caccataaac
     5401 ctaacaggcg acggaggcgt atcaataatg gctgtttggt ggagcatcaa tgcgcatcaa
     5461 tttatggtca gtcgaaatca tgactttcca gtttggatga tgttgtacaa cttcatgacc
     5521 aagatgttcg acgaaagctg tccggctaac cgcttttccc gttaaccgat tgaataatga
     5581 tttcaaaatg agttccctct cttattattc cctgctaaat ggttagttaa ccttcaccag
     5641 cgtgcgaccc tggatctggt tattaatgat ggcctcggca aacttcggtg cctctgccag
     5701 agatatctct tttgccgctt gggtatagaa tgattccggt aaatcggcga ccagtcgctg
     5761 ccaggcttgt gcgcggcgtg ctggtggcgt cattactgaa tccacccctt gcaaacggac
     5821 attacgcaga ataaatggca tgaccgtggt tggcagagta aaaccacccg ccagaccaca
     5881 ggccgccacg cagccgccgt aattcatttg cgccagcact tttgccagca ctttgtcgcc
     5941 aacggtgtca attgccccag cccagacttg tttttccaga ggacgggatt cagcaaactc
     6001 atcgcgagga agaatacggc tggcacctaa acttttcaga tattcatggg tactttcgcg
     6061 accggaaacg gcaacgacct gataacccaa cttatgcagc agcgtgacgg cggtactgcc
     6121 gacgccacca ctggcacccg tcacgacaac ctccccatcc tgcgggcgaa caccggcatc
     6181 ttccagcgcc atcacacaca gcatggcggt aaaaccggca gtaccgataa tcattgcttt
     6241 acgcgcgtcc agcccttgcg gcatggcaac cagccagtca cctttcactc gcgcctgttc
     6301 cgccagccca ccccagtggt tttcaccaac gccccagcca gtgagtaaca cctcctgacc
     6361 ggcatgaaaa cgcggatctt cgctggtgcg tacagttccg gcaaaatcaa tcccaggaat
     6421 catcggaaaa tttcggatga tttttccctt accggtaatc gccagcgcat ctttgtagtt
     6481 caggctcgac cagtgaacat caaccgtgac atcgccttcc ggcaggcgac tttcgtccag
     6541 agtctgtact gatgcgagag ttttgccgtc ctgctgttct aaaagtaacg cctgcataag
     6601 tggtcctcat gtgcatgatg gattggaaaa taatttctga agactatact cgctaatgga
     6661 aaagcaaagc cgatgaagcg caataaattg cgagataaat ctgatttgct agtatgcccg
     6721 cttcctcact atcggagtta acacaaggat gagattaacg acgaaatttt cggcctttgt
     6781 cacgctgctc accgggttaa caatttttgt gactttgctg ggctgttcgc taagtttcta
     6841 caacgccatt cagtataagt ttagtcatcg tgttcaggcg gtggcgacgg cgattgatac
     6901 ccacctagtg tcgaatgact tcagcacatt aaggccacaa attaccgaat taatgatgtc
     6961 ggcagatatc gttcgtgtag acctgctcca tggtgataag caggtttata ccctggccag
     7021 aaatggtagt tatcgtccgg ttggcaccaa cgatctgttt cgtgaactga gcgttccgtt
     7081 gataaagcat ccggggatgt cgctgcgtct ggtttatcag gatccgatgg gcaactattt
     7141 ccattcttta atgactaccg cgccgctcac gggggcgatt ggctttatca ttcttatgct
     7201 cttcctggcg gtacgctggt tacaacggca acttgccggg caagaattgc tggaaacccg
     7261 ggctactcgt atcttaaacg gtgagcgtgg ctctaatgtg ttgggaacca tctatgaatg
     7321 gccgcccaga accagcagtg cgctggatac gctgctttgt gaaattcaga acgcacgcga
     7381 acaacacagc cgtcttgata cgctgatccg ctcttatgcc gcccaggaca tgaaaaccgg
     7441 cctcaataac cgactctttt tcgataatca gttagcaacg ttactggaag atcaagagaa
     7501 agtaggtacc cacgggatcg tgatgatgat tcgtctgccg gatttcaata tgttgagtga
     7561 tacctggggg cacagccagg ttgaagaaca gttcttctct ctgacgaatc tgctatcgac
     7621 atttatgatg cgctaccctg gcgcactgct ggcgcgttac caccgcagtg attttgctgc
     7681 gctgttaccg caccggacgt taaaagaggc agagagcatc gccagtcagt taatcaaagc
     7741 cgtcgatacc ttgccgaaca ataaaatgct cgatcgcgac gatatgatcc acattggtat
     7801 ctgtgcctgg cgtagtggtc aggataccga gcaggtaatg gaacatgcag agtctgccac
     7861 gcgtaatgcg ggattgcagg gcggcaatag ctgggctatt tacgatgact cgttgcctga
     7921 aaaaggacgc ggtaatgttc gctggcgtac gcttatcgag caaatgctca gtcgcggcgg
     7981 cccgcgcctt tatcaaaaac cggcggttac tcgcgaaggc cgggttcatc atcgtgaact
     8041 catgtgccgc atcttcgatg gtaatgaaga ggttagctcg gcagagtata tgccgatggt
     8101 cttgcagttt ggtttatcgg aagagtatga ccgcctgcta atcagccgtc ttattccact
     8161 attgcgttac tggccagagg aaaatctggc gattcaggtt accgttgagt cgctgattcg
     8221 cccgcgtttt cagcgttggc tgcgcgatac gttaatgcaa tgtgaaaaat cgcaacgaag
     8281 acgcataatt attgaacttg cagaggccga tgttggtcaa catatcagtc gcttacaacc
     8341 tgttattcgt ttagtgaatg ctttaggggt acgggtagcc gtcaaccagg ctggtttgac
     8401 gctggtaagc accagttgga tcaaagaact taatgttgag ttactcaagc tccatccggg
     8461 gctggtcaga aacattgaga agcgaacgga gaatcagctg ctggttcaaa gcctggtgga
     8521 agcctgctcc gggaccagca cccaggttta cgccaccggc gtgcgttcgc gaagcgagtg
     8581 gcagaccctg attcagcgcg gtgttacagg tgggcaaggg gattttttcg cgtcctcaca
     8641 gccacttgat actaacgtga aaaaatattc acaaagatac tcggtttaac ctgccgttta
     8701 atccgttttc acgtagaata acgcgcgctg cgtctcatgg gagtgtgctt gtctgctcgc
     8761 cagattgttg cagcacatat gcagatgaat gaccttacgc ggttgcaaac aggcgaggaa
     8821 tgctgctgat gcattaagcc tttctggact caggcagaga tttgtaacaa aggaaacgaa
     8881 ctgcactaat tttcaccgta gcagatgatt tttgcgcctt gtcgctgctg cgtgtggttg
     8941 gtaaagtaag cggattttct tttccgcccc agctttcagg attatccctt agtatgttga
     9001 aaaaatttcg tggcatgttt tccaatgact tgtccattga cctgggtact gcgaataccc
     9061 tcatttatgt aaaaggacaa ggcatcgtat tgaatgagcc ttccgtggtg gccattcgtc
     9121 aggatcgtgc cggttcaccg aaaagcgtag ctgcagtagg tcatgacgcg aagcagatgc
     9181 tgggccgtac gccgggcaat attgctgcta ttcgcccaat gaaagacggc gttatcgccg
     9241 acttcttcgt gactgaaaaa atgctccagc acttcatcaa acaagtgcac agcaacagct
     9301 ttatgcgtcc aagcccgcgc gttctggttt gtgtgccggt tggcgcgacc caggttgaac
     9361 gccgcgcaat tcgtgaatcc gcgcagggcg ctggtgcccg tgaagtcttc ctgattgaag
     9421 aaccgatggc tgccgcaatt ggtgctggcc tgccggtttc tgaagcgacc ggttctatgg
     9481 tggttgatat cggtggtggt actactgaag ttgctgttat ctccttgaac ggcgtggttt
     9541 actcctcttc cgtgcgcatt ggtggtgacc gtttcgacga agctatcatc aactatgtac
     9601 gtcgtaatta cggttctctg atcggtgaag ccaccgcaga acgtatcaag cacgaaatcg
     9661 gttcggctta cccgggcgat gaagtacgtg aaatcgaagt tcgtggccgt aaccttgcag
     9721 aaggtgttcc acgcggcttt accctgaact ccaacgaaat cctcgaagca ctgcaggaac
     9781 cgctgaccgg tattgtgagc gcggtaatgg ttgcactgga acagtgcccg ccggaactgg
     9841 cttccgacat ctccgagcgt ggcatggtgc ttaccggtgg tggcgcactg ctgcgtaacc
     9901 ttgaccgttt gttaatggaa gaaaccggca ttccagtcgt tgttgctgaa gacccgctga
     9961 cctgtgtggc gcgcggtggc ggcaaagcgc tggaaatgat cgacatgcac ggcggcgacc
    10021 tgttcagcga agagtaatcg gatgcaggca ggggaagtgt ctgtttaccc tgcctggtct
    10081 gatacgagaa tacgcataac ttatgaagcc aatttttagc cgtggcccgt cgctacagat
    10141 tcgccttatt ctggcggtgc tggtggcgct cggcattatt attgccgaca gccgcctggg
    10201 gacgttcagt caaatccgta cttatatgga taccgccgtc agtcctttct actttgtttc
    10261 caatgctcct cgtgaattgc tggatggcgt atcgcagacg ctggcctcgc gtgaccaact
    10321 agaacttgaa aaccgggcgt tacgtcagga actgttgctg aaaaacagtg aactgctgat
    10381 gcttggacaa tacaaacagg agaacgcgcg tctgcgcgag ctactgggtt ccccgctgcg
    10441 tcaggatgag cagaaaatgg tgactcaggt tatctccacg gttaacgatc cttatagcga
    10501 tcaagttgtt atcgataaag gtagcgttaa tggcgtttat gaaggccagc cggttatcag
    10561 cgacaaaggt gttgttggtc aggtggtggc cgtcgctaaa ctgaccagcc gcgtgctgct
    10621 gatttgtgat gcgacccacg cgctgccaat ccaggtgctg cgcaacgata tccgcgtaat
    10681 tgcagccggt aacggttgta cggatgattt gcagcttgag catctgccag cgaatacgga
    10741 cattcgtgtt ggtgatgtgc tggtgacttc cggtctgggc ggtcgtttcc cggaaggcta
    10801 tccggtcgcg gttgtctctt ccgtaaaact cgatacccag cgcgcttata ctgtgattca
    10861 ggcgcgtccg actgcaggac tgcaacgttt gcgttatctg ctgctgctgt ggggcgcgga
    10921 tcgtaacggc gctaacccga tgacgccgga agaggtgcat cgtgttgcta atgaacgttt
    10981 gatgcagatg atgccgcagg tattgccttc gccagacgcg atggggccaa agttacctga
    11041 accggcaacg gggatcactc agccgactcc gcagcaaccg gcgacaggaa atgcagttac
    11101 tgcgcctgct gcgcccacac agcccgctgc taatcgctct ccacaaaggg ctacgccgcc
    11161 gcaaagtggt gctcaaccgc ctgcgcgtgc gccgggaggg caatagtggc gagctatcgt
    11221 agccagggac gctgggtaat ctggctctct ttcctcattg cgctgttgct gcaaatcatg
    11281 ccctggccgg ataacctgat tgttttccgg ccaaactggg tgttactcat cttgttgtat
    11341 tggatcctgg ccttgcctca tcgcgtaaat gtgggcacag gttttgtgat gggtgccata
    11401 ctggatctga tcagcggctc gacgcttggc gtacgcgtat tggcgatgag catcattgct
    11461 tacctggtgg cgctgaaata ccagcttttc cgcaacctcg cattatggca gcaggcgctg
    11521 gtcgtcatgt tgctttcgct ggtggtggat attattgttt tctgggcaga gtttttagtg
    11581 attaacgtct ctttcagacc ggaagtgttc tggagtagcg tagtcaatgg ggtgctctgg
    11641 ccgtggattt tcttgctgat gcgtaaagtc cgtcagcagt ttgcagtgca ataaaggttt
    11701 ctatgacttc tctgtattta gcttccggtt ctccgcgtcg tcaggagtta cttgcgcaac
    11761 ttggcgtaac ctttgaacgt attgttacgg gcattgagga gcagcgtcag ccgcaggaga
    11821 gcgcgcagca gtatgttgtg cgtctggcgc gcgagaaagc acaggcgggt gtcgcgcaaa
    11881 cggcgcagga tctcccggtg ctgggtgcgg atactatcgt tatcctgaac ggagaagtac
    11941 tggagaaacc gcgcgacgca gagcatgcgg cgcagatgtt gcgcaaatta tcgggtcaga
    12001 cccatcaggt aatgacggca gtggcgttgg ccgacagcca gcacattctc gattgcctgg
    12061 tggtcaccga tgtgactttc agaacgttaa cagacgaaga catcgcgggc tatgtcgcca
    12121 gcggtgaacc gttagataaa gcaggtgcat acggtattca ggggctgggt ggctgttttg
    12181 tcaggaagat aaatggcagc tatcacgccg tagtcggctt accgctggtt gaaacgtatg
    12241 aattattaag taattttaac gcactgcgtg agaaaaggga taaacatgac ggctgaattg
    12301 ttagtaaacg taacgccttc ggaaacgcga gtggcgtata ttgatggcgg tattctgcag
    12361 gaaattcata ttgaacgtga ggcgcgacgc ggaatagtag gcaatatcta caagggtcgt
    12421 gtaagtcgtg tacttccggg tatgcaggcg gcttttgtag atattgggct ggataaagcc
    12481 gcgtttcttc atgcatctga catcatgccg cacaccgaat gtgtggcggg tgaagaacaa
    12541 aagcaattca cggtgcgcga catctcggaa ctggttcgtc aggggcaaga tctgatggtg
    12601 caggtggtga aagatccact tggcactaag ggtgcgcgcc tgaccaccga tatcacgctg
    12661 ccttctcgct atctggtatt tatgccaggg gcttctcacg ttggggtttc ccaacgaatt
    12721 gaaagcgaat cagaacgtga acgcctgaaa aaagtggtcg cagagtattg cgacgagcag
    12781 ggcgggttta tcatccgtac cgcagctgaa ggggttggcg aggctgaact ggcctccgat
    12841 gccgcttatc tgaaacgcgt ctggaccaaa gttatggagc gtaaaaagcg cccacagacc
    12901 cgttatcagc tgtacggcga actggcgctg gcgcagcgtg ttctgcgtga tttcgccgac
    12961 gccgaactcg accgcattcg cgttgactca cgcctgactt acgaagcatt gctggagttt
    13021 acctcggagt acattcccga gatgaccagc aagctggagc attacaccgg acgccagccg
    13081 attttcgatc tctttgatgt cgaaaacgaa attcagcgag cgctggaacg caaagtagaa
    13141 ctgaaatccg gtggctatct gattatcgac cagaccgaag cgatgaccac cgtagacatc
    13201 aataccgggg cgtttgtcgg tcatcgcaat ctggacgaca ccattttcaa taccaatatt
    13261 gaagcgacgc aggctatcgc tcgccagtta cggttgcgta atctgggcgg gattatcatt
    13321 attgatttca tcgatatgaa taatgaagat caccgccgcc gcgtgctgca ctcgctggag
    13381 caggcgttga gcaaagaccg ggtgaaaacc agcgttaatg gtttttcggc gctggggctg
    13441 gtggagatga cgcgtaaacg cacccgcgaa agcattgagc acgtactatg taacgaatgc
    13501 ccaacctgcc acggtcgcgg aacggtgaaa accgtggaaa cggtatgcta tgaaatcatg
    13561 cgcgagattg ttcgtgtaca ccatgcttat gattccgacc gtttcctggt ctatgcttct
    13621 ccggcagtag ctgaagcctt gaaaggcgaa gagtcacact cgctggcgga agtggaaatt
    13681 ttcgttggta aacaggttaa agtacaaatt gaaccgctct ataaccagga gcagtttgac
    13741 gtcgtaatga tgtaaacaga tgctgggccg ccatccggca aagggttttt gagtcacatt
    13801 tttagcagac aaggagtgac gggtgaggcg attgccgggg attttactgc ttactggagc
    13861 cgcgctcgtt gtgatcgctg ccctgctggt tagcggcctg cgtattgctt taccgcatct
    13921 tgacgcctgg cgtccggaaa tcctcaacaa aatagaatcc gcgactggca tgccggtaga
    13981 agccagtcag ctctcagcca gctggcagaa ttttggtccg acgcttgaag cgcacgacat
    14041 ccgtgcagaa ctaaaagatg gcggcgaatt ttcggttaaa cgcgttactc tggcgctgga
    14101 tgtctggcag agcctgttac atatgcgctg gcagtttcgc gacctcactt tctggcagct
    14161 gcgctttcgc accaacactc ctatcaccag cggcggtggt aatgatagtc tggaagccag
    14221 tcacatcagc gatctgtttc ttcgtcagtt cgaccatttc gatctccgcg acagtgaagt
    14281 cagtttcctg acgccatccg gtcagcgcgc cgagctggcg atcccacagc tcacctggct
    14341 gaacgatcca cgtcgacacc gtgcagaagg ccaggtaagc ctctccagcc ttaccggaca
    14401 gcacggcgtg atgcaggtgc gcatggattt gcgcgatgat gaggggttgt taagcaatgg
    14461 tcgcgtctgg ctccaggcgg atgacatcga cctgaagccg tggctcggta aatggatgca
    14521 ggacaatatt gcgctggaaa cggcacagtt ctcccttgaa ggctggatga cgatcgacaa
    14581 aggcgatgta accggcggtg acgtctggct gaaacagggc ggtgccagct ggttgggcga
    14641 gaagcaaacg catacgctgt cggtggataa tctgaccgcg catattacgc gtgaaaatcc
    14701 aggctggcag ttctctattc ccgatacacg gatcacgatg gacggcaaac cctggccgag
    14761 cggagcattg acgctggcct ggataccgga acaggacgtt ggcggcaaag acaataaacg
    14821 cagtgacgaa ctccggattc gcgccagtaa tctggagctg gcaggcctgg agggcatacg
    14881 cccgctggcc gcgaaacttt cacctgcact gggtgatgtt tggcgctcca cacaaccgag
    14941 cggcaagatt aacacgctgg cgctggatat cccgcttcag gcggcagaca agacccgttt
    15001 tcaggcatcg tggagcgatc tggcctggaa gcaatggaaa ttattaccgg gtgcggaaca
    15061 cttctccggg acgctttccg gcagcgtaga aaatggtttg cttactgcgt cgatgaagca
    15121 ggcaaagatg ccttacgaaa cggtattccg tgcgccactg gaaatcgccg acggccaggc
    15181 aacgataagc tggctgaaca atgacaaagg tttccagctg gatgggcgta atattgacgt
    15241 taaagccaaa gccgtccatg cgcgcggcgg ttttcgttac ctgcaaccag ctaacgatga
    15301 accctggctg ggtattctgg ctggcatcag taccgatgat ggttcacaag cctggcgcta
    15361 tttcccggaa aacttgatgg gtaaagacct ggttgattac ttaagtggcg cgattcaggg
    15421 cggtgaagcg gataacgcga cgctggttta tggtggcaat ccgcaactct tcccctataa
    15481 acacaacgaa ggtcagtttg aagtgctggt accgctgcgc aacgcgaagt ttgccttcca
    15541 gccggactgg cctgcattaa ctaaccttga tattgaactg gactttatta acgacggttt
    15601 atggatgaaa accgatggcg ttaatctggg cggcgtgcgc gcaagtaatc tcaccgcagt
    15661 gatccctgac tactcaaaag aaaaactgct gattgacgct gacattaaag gtccgggtaa
    15721 agccgttggc ccttactttg atgagacacc gctgaaagat tctctgggtg cgaccctgca
    15781 agaactccag ctcgacggcg atgtgaatgc tcgcttacat cttgatatcc cgctgaacgg
    15841 cgaactggta accgcgaaag gtgatgtgac gctgcgtaat aacagtctgt ttatcaaacc
    15901 actcgacagc accctgaaaa atttgagcgg taaattcagc tttatcaatg gcgatctgca
    15961 aagtgaacca ctgacagcaa gctggtttaa tcagccgttg aacgtggatt tttccaccaa
    16021 agaaggggca aaagcctacc aggtcgcggt gaatctcaac ggtaactggc aaccagcgaa
    16081 aaccggcgtt ctgcctgaag cggtgaacga agcattaagt ggcagcgtgg cgtgggatgg
    16141 taaagtgggc attgatctgc cttatcatgc tggcgcgacc tataacgtag agctgaacgg
    16201 cgacctgaag aatgtgagca gtcacttacc ttcaccgtta gccaaacctg cgggtgaacc
    16261 actgccggta aacgttaagg ttgatggcaa tctcaacagc tttgagttaa ccggacaggc
    16321 tggtgcagat aaccatttca atagccgctg gttgctcggt caaaagctga cgctcgaccg
    16381 tgctatttgg gcggcagaca gtaaaacgct cccgccgttg ccggaacaaa gtggcgttga
    16441 actcaatatg ccgccgatga atggtgccga gtggctggcc ctgttccaga aaggcgctgc
    16501 ggagagtgtc ggtggtgcag cgagtttccc acaacacata acgttacgta cgcctatgtt
    16561 gtcgctggga aatcagcaat ggaataacct gagcattgtt tcgcaaccga cggcaaatgg
    16621 caccctggtt gaggcgcaag ggcgtgaaat caacgccacg ctggcgatgc gtaataacgc
    16681 gccgtggctg gcgaatatca aatatcttta ttacaaccca agcgtggcga aaactcatgg
    16741 tgactcaacg aattcatcac ctttcccgac aacggagcgc attagcttcc gtggctggcc
    16801 ggacgcacaa atacgatgcg cagagtgctg gttctggggg caaaaattcg ggcgcattga
    16861 cagtgatatc accatttctg gcaatacatt aacgctgact aatggactga ttgatactgg
    16921 tttttcgcgg ctcactgccg atggtgaatg ggttaacaat ccggggaatg aacgcacctc
    16981 gctgaaagga aaactgcgtg ggcagaaaat tgatgccgcc gcagaatttt ttggtgtcac
    17041 gacgcccata cgccagtcgt catttaatgt ggattacgat ttacactggc gcaaagcacc
    17101 ctggcaacca gatgaggcga cgttgaatgg catcattcat actcaactgg gtaaaggcga
    17161 aattaccgaa atcaataccg gacatgccgg gcaattgctg cgcttattga gcgtagatgc
    17221 cctgatgcgt aagctgcgtt ttgatttcag agacactttt ggcgaagggt tctattttga
    17281 ctccattcgc agcaccgcgt ggattaaaga cggcgttatg cacaccgacg acacgctggt
    17341 ggatggcctg gaagcggaca tcgctatgaa agggtcggta aatctggtac gtcgcgacct
    17401 gaatatggaa gcggttgtcg caccagagat ttctgcgacg gtgggtgtgg ctgcggcctt
    17461 tgcggttaac cccattgttg gcgcggcagt gtttgccgcc agtaaagtgc tgggaccgct
    17521 gtggagcaaa gtctccattt tgcgctatca catttcgggt ccgctggacg atccgcaaat
    17581 caacgaagtg ttgcgacaac cgcgtaaaga aaaagcgcaa tgatttgacg agggcgcgta
    17641 attgccccaa tctcatagga taatcgttgc caaaggccaa cgagccagaa cataaccgta
    17701 ggccggacag ggcgttcacg ccgcatccgg cagccgtaaa aaatcctcta ctgcagtaac
    17761 taacgagtag caaaaacgat gagtctgaac ctggtaagtg aacaattgct agcggcgaac
    17821 ggcctgaaac atcaggactt gttcgcgatc ctcggtcaac tggccgaacg tcgccttgat
    17881 tatggcgatc tctattttca gtcgagctat cacgaatcct gggttttaga agaccgtatt
    17941 attaaagatg gttcttacaa catcgatcag ggcgttggcg tgcgtgcaat cagtggtgaa
    18001 aaaaccggat ttgcttatgc tgaccaaatc agcctgctgg cgctggaaca gagtgcacaa
    18061 gcggcgcgca ccatcgtccg tgatagtggt gatggtaaag tacagacgct gggcgcggta
    18121 gagcatagcc cgttgtatac ctcggtagat ccgctgcaaa gcatgagccg tgaagagaag
    18181 ctggatatcc tgcgtcgcgt cgataaggtt gcccgcgaag cggacaagcg cgtacaggaa
    18241 gtgactgcca gcctcagtgg tgtctatgaa ttaattctgg ttgcggccac cgacggcacg
    18301 ctagcggcgg atgtccgtcc gctggtgcgt ctttccgtga gcgttctggt cgaagaagat
    18361 ggcaaacgcg aacgcggtgc cagtggcggc ggcggtcgtt ttggttatga gttcttcctt
    18421 gccgatctcg acggcgaagt ccgtgcggat gcatgggcaa aagaagcagt gcgtatggcg
    18481 ctggtcaatc tttctgccgt tgctgcacca gcgggcacca tgccggtagt acttggcgca
    18541 ggttggccgg gcgtgctgtt gcatgaagcg gtaggtcacg gtctggaagg cgacttcaac
    18601 cgccgtggca cttcagtatt tagtggacag gttggggagc tggtggcttc agaactgtgt
    18661 accgtggttg atgacggtac gatggtcgat cgccgtggtt cggtggcgat tgatgacgaa
    18721 ggtacgccag gccagtacaa cgtgctgatt gagaatggca tcctgaaagg ctacatgcag
    18781 gataaactca acgcgcgttt gatggggatg acgccgactg gcaacggtcg ccgtgaatcc
    18841 tacgcccatc tgcccatgcc gcgtatgacc aacacctata tgctgccggg taaatcgacc
    18901 ccgcaggaaa ttattgaatc cgttgagtac ggtatctatg caccgaactt tggtggcggt
    18961 caggtggata tcacctccgg caaattcgtt ttctccactt cagaagcata tctgattgaa
    19021 aacggtaaag taacgaagcc ggtgaaaggc gcaacgttga ttggttccgg tatcgaaacc
    19081 atgcagcaga tttcgatggt gggtaacgac ctgaaactgg ataacggcgt gggtgtctgc
    19141 ggtaaagaag ggcaaagttt gccggttggc gtgggccagc caacgttgaa agtcgataac
    19201 ctgactgttg gcggtactgc gtaataattt cagtattttc agaaggataa ttaatctttc
    19261 tacgtgcacg aacggtcccc tcgctcctct ggggttaggg tgaggggaac ccgttggcac
    19321 aggtttgtac aacgtaacag tacaatatga attacttctc tttcccgcgc ccgtgcatct
    19381 cctgaaacaa tttaccgacc tcaacaaaat aatccgtcag cgagttgatc acgacctgta
    19441 ccttcagcgg cagcttatct ttttcggtat ataacgcata aaccgggcgt ggatctgact
    19501 gataacgcgg cagcaggatc tccagttccc cacgattgat ctcgttgatc acccacatca
    19561 gcggcacgta ggcgatcccg gcacccgccg tcagccagcg caccagcgtc atcggatcat
    19621 tagtcacaaa tcttccttgt gggatcaggc gagtcgagat cccttccggt gcgatcagtt
    19681 caaattcatt gtcgggccgc acgctgtatt caagccatga atgactactc aaatcggcgg
    19741 gtttttccgg tatgccgtat tgtgtgagat agcttttcgc ggcgcacacc accatcggca
    19801 tcgcgcccag acggcgggaa aacaggctgg aatcctgcaa cgcgccgacg cggatcacca
    19861 catccagacc atcggcaatc aggtcggggg caggaattcc ggtaaccaga ttgacgctca
    19921 aacctgggta ttctttcagc atttttgctg tcagcccggc gagaacattt tgtgccatag
    19981 ttgaagaaca gccaatgcgc agcgtcccga tgggggtgtt attgaaggca tacagttgct
    20041 catgaacatc ctgcacttca tggagcatcc gacggcagcc ctggtagtaa attctgccgg
    20101 cttcagtcag gccaatgctg cgtgtactac ggtttaacag ctttacctgt aactcatctt
    20161 ccagttttga caccgtctga ctgatggacg aaacgctcat ctgtagctgt ctggcggcgg
    20221 cggtaaaaga gccaaattca actactttgg caaacaccga catgcgtttt agtcgttcca
    20281 ttattcactc tgacttaaaa gtgatttaga tcacataata tagataacag cataacagtt
    20341 acgctaatat attaaatatc aatctacagc aatgttgctc tcgcccggct ttccatgccc
    20401 ttctctgcgg cgacagatgc tgaaaataat aacgcctgct ctctctatac aaccaaggtc
    20461 aacatgagtc tgtttcccgt tatcgtggtg tttgggctgt ccttcccacc gatatttttt
    20521 gaattgcttt tatcactggc gattttctgg ctggtgcgcc gggtacttgt gccaacaggt
    20581 atctacgact ttgtctggca tccggcgttg ttcaacaccg cgctctattg ctgcttgttt
    20641 tatttgatat cgcgactgtt cgtttgaggt tgaagtgaaa acactaataa gaaaattctc
    20701 ccgtacggcc atcacggtcg tattagtcat tctggccttc atcgcaattt ttaatgcctg
    20761 ggtctattac accgaatccc cgtggacgcg tgacgcgcgc tttagcgctg acgtcgttgc
    20821 gatcgcgccg gacgtttctg gactcattac ccaggtgaat gttcatgata accagctggt
    20881 gaaaaaagga caggtactgt tcaccatcga ccagccgcgc tatcaaaagg cgcttgagga
    20941 agcgcaagcc gatgttgctt attatcaggt actggcacag gagaaacgcc aggaggcagg
    21001 acgtcgtaac cgtctcggtg tgcaggcgat gtctcgcgaa gagatcgatc aggccaacaa
    21061 cgtactacaa acggttctgc atcagttagc gaaagcgcag gcgacccgtg atctggcaaa
    21121 actggatctt gaacgtacag tcatccgcgc gccagcagat ggctgggtga ccaacctcaa
    21181 cgtctatacc ggtgagttta ttactcgagg atcaacggcg gttgcgctgg tgaaacagaa
    21241 ctccttctat gtactggcct atatggaaga aactaagctg gaaggggtgc gccctgggta
    21301 tcgtgcggag atcacgccgc ttggcagtaa caaagtgctg aaagggactg ttgatagtgt
    21361 tgccgcaggg gtcaccaacg ccagcagcac gcgtgacgac aaagggatgg cgaccatcga
    21421 ctctaacctt gaatgggtac gtttggcgca acgtgttccg gtacgtattc gtctcgacaa
    21481 ccagcaagag aacatctggc ctgcgggcac cactgctaca gtggtggtca ctggcaaaca
    21541 agatcgcgac gaaagccagg attcgttctt ccgtaaaatg gcccatcgcc tgcgtgagtt
    21601 tggttaatca cgatgggtat tttctccatt gctaatcaac atattcgctt tgcggtaaaa
    21661 ctggcgaccg ccattgtact ggcgctgttt gttggctttc acttccagct ggaaacgcca
    21721 cgctgggcgg tactgacagc ggcgattgtt gccgccggtc cggcctttgc tgcgggaggt
    21781 gaaccgtatt ctggcgctat tcgctatcgt ggctttttgc gcatcatcgg cacatttatt
    21841 ggctgtattg ctggactggt aatcatcatt gcgatgatcc gcgcaccatt attgatgatt
    21901 ctggtgtgct gtatctgggc cggtttttgt acctggatat cctcgctggt acgaatagaa
    21961 aactcgtatg cgtgggggct ggccggttat accgcgctga tcattgtgat caccattcag
    22021 ccggaaccat tgcttacgcc gcagtttgcc gtcgaacgtt gtagcgagat cgttatcggt
    22081 attgtgtgtg cgattatggc ggatttgctc ttttctccgc gatcgatcaa acaagaagtg
    22141 gatcgagagc tggaaagttt gctggtcgcg caatatcaat taatgcaact ctgtatcaaa
    22201 catggcgatg gtgaagttgt cgataaagcc tggggcgatc tggtgcgacg caccacggcg
    22261 ttacaaggta tgcgcagcaa cctgaatatg gaatcttccc gctgggcgcg ggccaatcga
    22321 cgtttaaaag cgatcaatac gctatcgctg acgctgatta cccaatcctg cgaaacttat
    22381 cttattcaga atacgcgccc ggaattgatc actgatactt tccgcgaatt ttttgacacg
    22441 ccggtagaaa ccgcgcagga cgtccacaag cagctcaaac gcctgcggag agttatcgcc
    22501 tggaccgggg aacgggaaac gcctgtcacc atttatagct gggtcgcggc ggcaacgcgt
    22561 tatcagcttc tcaagcgcgg cgttatcagt aacacaaaaa tcaacgctac tgaagaagag
    22621 atcctgcaag gcgaaccgga agtcaaagta gagtcagccg aacgtcatca tgcgatggtt
    22681 aacttctggc gaaccacact ttcctgcatt ctggggacgc ttttctggct gtggacgggc
    22741 tggacttccg gcagtggtgc aatggtgatg attgcggtcg tgacgtcact ggcaatgcgt
    22801 ttgccgaatc cacgcatggt ggcgatcgac tttatctatg ggacgctggc ggcgctgccg
    22861 ttagggctgc tctacttttt ggtgattatc cctaataccc aacaaagcat gttattgctg
    22921 tgtattagcc tggcagtgct gggatttttc ctcggtatag aagtacagaa acggcgactg
    22981 ggctcgatgg gggcgctggc cagcaccata aatattatcg tgctggataa cccgatgact
    23041 ttccatttca gtcagtttct cgacagcgca ttagggcaaa tcgtcggctg tgtgctcgcg
    23101 ttcaccgtta ttttgctggt gcgggataaa tcgcgcgaca ggactggacg tgtactgctt
    23161 aatcagtttg tttctgccgc tgtttccgcg atgactacca atgtggcacg tcgtaaagag
    23221 aatcacctcc cggcacttta tcagcagctg tttttgctga tgaataagtt cccaggggat
    23281 ttgccgaaat ttcgcctggc gctgacgatg attatcgcgc accagcgcct gcgtgatgcg
    23341 ccgatcccgg ttaacgagga tttatcggcg tttcaccgac aaatgcgccg cacagcagac
    23401 catgtgatat ctgcccgtag cgatgataaa cgtcgtcggt actttggcca gttgctggaa
    23461 gaacttgaaa tctaccagga aaagctacgc atctggcaag cgccaccgca ggtgacggaa
    23521 ccggttcatc ggctggcggg gatgctccat aagtatcaac atgcgttgac cgatagttaa
    23581 gtcaaaaccg acgcccgaag cgtcggtttt ttcatggcta tacttagcga tgaacggcag
    23641 aactcgccgt gaaacgtgac ggtggcaaca gatgaatatt tatacctttg attttgatga
    23701 gattgagagt caggaggatt tttatcgcga cttcagccaa gcctttggtc tggcgaaaga
    23761 taaggtacgc gatctcgact cactatggga tgtgttaatg aacgatgtcc tgccgctacc
    23821 acttgagatt gaatttgttc atctgggaga gaaaacgcgt cgccgttttg gcgcgttaat
    23881 attgctgttt gatgaggcag aggaagagct ggaagggcat ttgcgtttta atgttcgtca
    23941 ttagcgcaaa aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt
    24001 atttgtacag ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt
    24061 aggccgttgc gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg
    24121 aagacgccac accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag
    24181 catcaatgga gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag
    24241 taacagtggt tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg
    24301 tttcgtgacc catatcacaa aatcaagtat acactaatta ttgaactaat taata
//
LOCUS       DAZKGF010000072        23681 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZKGF010000072 DAZKGF010000000
VERSION     DAZKGF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.072"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            21..1535
                     /gene="caiT"
                     /locus_tag="ACHOQV_002628"
     CDS             21..1535
                     /gene="caiT"
                     /locus_tag="ACHOQV_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFP9630986.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1566..2708
                     /gene="caiA"
                     /locus_tag="ACHOQV_002629"
     CDS             1566..2708
                     /gene="caiA"
                     /locus_tag="ACHOQV_002629"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFP9630987.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2836..4053
                     /gene="caiB"
                     /locus_tag="ACHOQV_002630"
     CDS             2836..4053
                     /gene="caiB"
                     /locus_tag="ACHOQV_002630"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFP9630988.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4126..5679
                     /gene="caiC"
                     /locus_tag="ACHOQV_002631"
     CDS             4126..5679
                     /gene="caiC"
                     /locus_tag="ACHOQV_002631"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFP9630989.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYVLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMVLPADDGVSSFTQRKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHCLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5788..6573
                     /gene="caiD"
                     /locus_tag="ACHOQV_002632"
     CDS             5788..6573
                     /gene="caiD"
                     /locus_tag="ACHOQV_002632"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFP9630990.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEAQRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6579..7169
                     /gene="caiE"
                     /locus_tag="ACHOQV_002633"
     CDS             6579..7169
                     /gene="caiE"
                     /locus_tag="ACHOQV_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFP9630991.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGVGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAIIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYK
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7288..7683)
                     /gene="caiF"
                     /locus_tag="ACHOQV_002634"
     CDS             complement(7288..7683)
                     /gene="caiF"
                     /locus_tag="ACHOQV_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFP9630992.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7944..11165)
                     /gene="carB"
                     /locus_tag="ACHOQV_002635"
     CDS             complement(7944..11165)
                     /gene="carB"
                     /locus_tag="ACHOQV_002635"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP9630993.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11183..12331)
                     /gene="carA"
                     /locus_tag="ACHOQV_002636"
     CDS             complement(11183..12331)
                     /gene="carA"
                     /locus_tag="ACHOQV_002636"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFP9630994.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(12787..13608)
                     /gene="dapB"
                     /locus_tag="ACHOQV_002637"
     CDS             complement(12787..13608)
                     /gene="dapB"
                     /locus_tag="ACHOQV_002637"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP9630995.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(13775..14689)
                     /gene="rihC"
                     /locus_tag="ACHOQV_002638"
     CDS             complement(13775..14689)
                     /gene="rihC"
                     /locus_tag="ACHOQV_002638"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFP9630996.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(14755..15705)
                     /gene="ispH"
                     /locus_tag="ACHOQV_002639"
                     /gene_synonym="lytB"
     CDS             complement(14755..15705)
                     /gene="ispH"
                     /locus_tag="ACHOQV_002639"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFP9630997.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKGVNCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15707..16156)
                     /gene="fkpB"
                     /locus_tag="ACHOQV_002640"
     CDS             complement(15707..16156)
                     /gene="fkpB"
                     /locus_tag="ACHOQV_002640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9630998.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16264..16758)
                     /gene="lspA"
                     /locus_tag="ACHOQV_002641"
     CDS             complement(16264..16758)
                     /gene="lspA"
                     /locus_tag="ACHOQV_002641"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP9630999.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16758..19574)
                     /gene="ileS"
                     /locus_tag="ACHOQV_002642"
     CDS             complement(16758..19574)
                     /gene="ileS"
                     /locus_tag="ACHOQV_002642"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP9631000.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSLSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCANAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19617..20558)
                     /gene="ribF"
                     /locus_tag="ACHOQV_002643"
     CDS             complement(19617..20558)
                     /gene="ribF"
                     /locus_tag="ACHOQV_002643"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFP9631001.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20566..20784)
                     /gene="yaaY"
                     /locus_tag="ACHOQV_002644"
     CDS             complement(20566..20784)
                     /gene="yaaY"
                     /locus_tag="ACHOQV_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFP9631002.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            20887..21150
                     /gene="rpsT"
                     /locus_tag="ACHOQV_002645"
     CDS             20887..21150
                     /gene="rpsT"
                     /locus_tag="ACHOQV_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP9631003.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            complement(21246..22151)
                     /gene="nhaR"
                     /locus_tag="ACHOQV_002646"
     CDS             complement(21246..22151)
                     /gene="nhaR"
                     /locus_tag="ACHOQV_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFP9631004.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(22211..23377)
                     /gene="nhaA"
                     /locus_tag="ACHOQV_002647"
     CDS             complement(22211..23377)
                     /gene="nhaA"
                     /locus_tag="ACHOQV_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFP9631005.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNHADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         5749 a         6345 c         6011 g         5576 t
ORIGIN      
        1 tttcacagtg gaattaactc atgaagaatg aaaagagaaa aacgggaata gaaccgaagg
       61 ttttctttcc gccgttaata atcgtcggca tactttgttg gcttacagtc agagatctgg
      121 atgcggcgaa tgtcgttatt aatgctgtat tcagttacgt caccaatgta tggggatggg
      181 catttgaatg gtatatggtg gtgatgcttt tcggctggtt ctggctggtg tttggcccgt
      241 atgccaaaaa gcgtttaggt aacgaaccac cagaatttag taccgccagt tggatcttta
      301 tgatgttcgc ctcctgtacg tctgctgccg tactgttctg gggatcgatt gagatctact
      361 actacatctc caccccgccg tttggcttag aaccgaactc aacaggcgcg aaagagttgg
      421 ggctggctta cagcttgttc cactggggac ctctgccgtg ggccacttac agcttccttt
      481 cagtcgcctt cgcttacttc ttctttgtcc gcaaaatgga agtgattcgc cccagctcca
      541 ccctggtgcc gctggtaggt gaaaaacacg ccaaagggtt gttcggcact atcgtcgaca
      601 acttctatct cgtcgccttg atcttcgcga tgggtaccag tctgggcctt gccacgccgc
      661 tggtgaccga gtgtatgcaa tggttgtttg gcattccgca taccctgcaa ctggacgcta
      721 tcatcattac ctgctggatt atcctcaacg ccatttgcgt cgcttgcggt ctgcaaaaag
      781 gggtacgtat cgccagtgac gtgcgtagtt acctgagctt cctgatgctg ggttgggtgt
      841 tcatcgtcag cggtgccagc ttcatcatga actacttcac cgattcggtg gggatgttgc
      901 tgatgtatct gccgcgcatg ttgttctata ccgatcccat cgctaaaggc ggcttcccgc
      961 agggctggac cgtgttctac tgggcatggt gggtgattta cgctatccag atgagcatct
     1021 tcctcgcccg catctcccgt ggtcgtaccg tgcgtgaact gtgcttcggc atggtgctgg
     1081 ggctgacagc gtcaacctgg atcctgtgga ctgtactcgg tagtaacact ctgctgttga
     1141 tagataaaaa catcatcaac attccaaatc tgatcgaaca gtacggtgtg gcgcgcgcca
     1201 tcatcgaaac ctgggccgct ctgccactca gcaccgccac catgtggggc ttcttcatcc
     1261 tctgctttat tgccaccgtt acgctggtta acgcctgctc ttataccctg gcgatgtcca
     1321 cttgccgcga agtacgcgat ggtgaagaac caccgctgct ggtgcgtatc ggctggtcaa
     1381 ttctggttgg cattatcggt attgttctgc tggcgctcgg cggcctgaaa ccgattcaaa
     1441 ccgccattat cgccggagga tgcccgctgt tcttcgtcaa cattatggtg acgctctcct
     1501 ttattaaaga cgcgaaacag aactggaaag attaattaac ccccaaaata tcaagaggtt
     1561 gaaagatgga ttttaattta aatgatgagc aggaactgtt tgtcgccggt atccgcgaac
     1621 tgatggccag cgaaaactgg gaggcctatt ttgccgagtg cgaccgtgac agcgtctacc
     1681 cggaacgttt tgtcaaagca ctggcggata tgggtatcga cagtctgctg attcctgaag
     1741 agcacggtgg tcttgacgcg ggttttgtta ctctcgccgc cgtgtggatg gagctgggac
     1801 gtctgggggc accaacctat gtgctgtacc agttgccggg cgggttcaat accttcctgc
     1861 gcgaaggcac acaagagcag atcgacaaga ttatggcttt ccgcggcacc ggtaagcaga
     1921 tgtggaactc agcgattacc gaaccgggcg cgggctccga cgtgggtagc ctgaaaacga
     1981 cttatacccg tagaaatggt aagatttatc ttaatggtag taagtgtttt attaccagca
     2041 gcgcctacac cccgtacatc gtggtgatgg cgcgcgacgg ggcttctccg gacaaacctg
     2101 tctacaccga atggtttgtt gatatgagca aaccgggcat caaagtgacc aaacttgaga
     2161 agctcggtct gcgtatggat agctgctgtg aaatcacttt tgacgacgtg gaactggacg
     2221 agaaagacat gttcggtcgg gaaggtaacg gatttaaccg cgtcaaagaa gagttcgacc
     2281 atgaacgttt cctggtagcc ctgaccaact acggtacggc gatgtgcgcc tttgaagatg
     2341 cggcgcgcta cgccaaccag cgcgtgcagt ttggcgaggc tattggtcgt ttccagttga
     2401 ttcaggaaaa attcgcccac atggcgatca aattaaactc catgaaaaac atgctgtatg
     2461 aagcagcgtg gaaagcagac aacggcacca tcacctctgg cgatgcagcg atgtgcaaat
     2521 acttctgcgc caatgcggca tttgaagtgg tggatagcgc aatgcaggtg ctgggcggtg
     2581 tcgggattgc gggcaaccac cgcatcagcc gcttctggcg tgacctgcgt gtagaccgcg
     2641 tttccggagg atctgacgaa atgcagatcc tgacgctggg tcgcgcggtg ctgaagcaat
     2701 accgttaagt ccgtgcgctg cctgatgcga cgctgacgcg tcttatcagg cctacgggag
     2761 cacgaagatg gccggataag gcgtttacgc cgcatccggc agtcatgcgc tacataaatt
     2821 tttcaggagc taataatgga tcatctaccc atgccgaaat tcgggccgct ggccggatta
     2881 cgcgttgtct tctccggtat cgaaatcgcc gggccgttcg ccggacaaat gttcgcagaa
     2941 tggggggcgg aagttatctg gatcgagaac gtcgcctggg ccgacaccat tcgcgttcaa
     3001 ccgaactacc cgcagctctc ccgccgcaat ttgcatgcgc tgtcgttaaa tattttcaaa
     3061 gatgaagggc gcgaagcgtt tctgaaatta atggaaacca ccgatatctt catcgaagcc
     3121 agtaaaggtc cggcctttgc ccgtcgtggc attaccgatg aagtcctgtg gcagcacaac
     3181 ccaaaactgg ttatcgctca cctttccggt tttggtcagt atggcaccga ggagtacacc
     3241 aatcttccgg cctataacac tatcgcccag gccttcagtg gttacctgat tcagaacggt
     3301 gatgttgacc agccaatgcc tgccttcccg tataccgccg attacttttc tggcctgacc
     3361 gccaccacgg cggcgcttgc agcactgcat aaagtgcgtg aaaccggcaa aggcgaaagc
     3421 atcgacatcg ccatgtatga agtgatgctg cgtatgggcc agtacttcat gatggattac
     3481 ttcaacggcg gcgaaatgtg cccgcgcatg accaaaggta aagatcccta ctacgccggt
     3541 tgcggtctgt ataaatgcgc cgacggctac atcgtgatgg aactggtggg cattactcaa
     3601 attgcagagt gctttaaaga tattggcctc gcacacctgc tcggtacgcc ggaaatcccg
     3661 gaaggcactc agcttatcca ccgtatcgaa tgcccttacg gcccactggt agaagagaaa
     3721 ctcgatgcct ggctggcggc acataccatc gcagaagtta aagaacgctt tgccgaactg
     3781 aatatcgcct gtgccaaagt actgaccgta ccggaactgg aaagcaatcc acagtatgtg
     3841 gctcgcgaat caatcactca gtggcaaacg atggatggtc gcacctgcaa agggccgaat
     3901 atcatgccga aattcaaaaa taaccccgga caaatctggc gcggaatgcc ctcacatggc
     3961 atggacacgg ctgctatttt gaaaaatatc ggctacagcg aaaacgacat tcaggagttg
     4021 gtcagcaaag gtctggccaa agttgaggac taaacgcgtg gcgtcagtca gcttgcctga
     4081 ctagcgcccg ctcaataaca aacgaacaaa atggatagag gtgcaatgga tatcattggc
     4141 ggacaacatc tacgtcaaat gtgggacgat ctggcggacg tttacggtca taaaacggcg
     4201 ctgatttgtg aatccagcag cggagtcgtt aaccggtata gttatcttga gttaaatcag
     4261 gagattaacc gcacggcaaa cctgttttat gtgctgggga ttcgcaaagg cgacaaggtt
     4321 gctctacatc tcgacaactg cccggaattt atcttttgct ggttcgggtt ggcaaaaatt
     4381 ggcgcgatta tggtgccgat taacgcccgc ctgttgcgcg aagaaagcac gtggatcctg
     4441 caaaatagcc aggcgtgcct gctggtgacc agtgcgcaat tttatcctat gtatcaacag
     4501 attcagcagg aagatgcctc tcagttgcgg cacatttgcc tgacagatat ggtacttccc
     4561 gctgatgatg gcgtgagttc gtttactcaa cggaaaaatc aacaacctgc caccttgtgc
     4621 tatgcaccgc cgctatcgac tgacgatacg gcggaaattc tcttcacctc cggcaccacc
     4681 tcccgaccga aaggggtggt gataactcat tacaacctgc gcttcgctgg atattactcc
     4741 gcctggcagt gtgcactgcg tgacgatgac gtctacctga cggtaatgcc tgcgtttcat
     4801 atcgattgcc agtgtactgc ggcgatggcg gcgttttctg ccggggccac ctttgtgctg
     4861 gtcgagaaat acagcgcccg cgccttctgg ggacaggtgc agaagtaccg cgccaccatt
     4921 accgaatgta ttccgatgat gattcgtacg ttgatggtgc agccgccttc agccaacgat
     4981 cggcaacact gcctgcggga agtgatgttt tatctcaacc tgtcggagca ggaaaaagac
     5041 gcgttttgtg aacgcttcag cgttcgcttg ctgacgtctt atgggatgac ggaaaccatt
     5101 gtgggcatta tcggcgatcg ccctggcgat aaacggcgct ggccgtcgat tggtcgggcg
     5161 ggcttttgct acgaagcgga gatccgcgac gatcacaatc gtcccctccc ggctggtgag
     5221 ctcggtgaaa tctgtattaa aggcgtacca gggaaaacca tcttcaaaga gtattttctc
     5281 aacccgaaag ccactgcgaa agtgctggaa gccgatggct ggctacatac cggcgatacc
     5341 ggataccgcg acgaagaggg ctttttttat ttcgtcgatc gccgctgcaa tatgatcaaa
     5401 cgcggtggcg agaatgtctc ctgcgtggag ctcgaaaata tcatcgccac ccatccaaaa
     5461 attcaggata tcgtggttgt gggtattaaa gattcgattc gcgatgaagc catcaaagca
     5521 tttgtggtgc tgaatgaagg tgaaacattg agcgaagagg aattcttctg cttctgtgaa
     5581 cagaatatgg cgaaatttaa agtgccctct tatctggaga tcagaaaaga tctgccacgt
     5641 aattgctcgg ggaaaataat tagaaagaat ctgaaataaa tgaaacggca gggaactacc
     5701 ctgtcagcta ataatcacgc tctaaaacaa tatgcatttt ttgcagggat gttgtcgtcc
     5761 ctgaaaaagc aaaaatggag aaaaggaatg agtgaatcat tacatctgac ccgcaatgga
     5821 tcaattctgg aaattaccct tgatcgtcca aaagcgaatg ctattgatgc aaaaaccagc
     5881 tttgaaatgg gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg tgtcgccatt
     5941 attaccggtg ccggagagaa attcttttcc gcgggctggg atttaaaagc ggcagcagaa
     6001 ggcgaagcac cggatgctga ctttggcccg ggaggttttg cgggattaac cgaaattttc
     6061 aatctcgaca aaccggttat cgcagctgtg aacggctacg cctttggcgg cggtttcgaa
     6121 ctggcgctgg cggcagattt tattgtttgt gccgataatg ccagcttcgc cctgccggaa
     6181 gccaaactgg gcatcgttcc tgacagcggc ggcgtgctgc gtctgccgaa gatcctgccg
     6241 cctgccatcg tcaatgaaat ggtgatgacc ggcagacgaa tgggcgcaga agaggcgcag
     6301 cgttggggga tagtcaaccg cgtggttagc caggcggaac tgatggataa cgcccgcgaa
     6361 ctggcgcagc agctggttaa cagcgctcca ctggcgattg cggcgctgaa agagatctac
     6421 cgcaccacca gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag cggcgtgttg
     6481 aaacactatc catcggttct gcattcggaa gatgccattg aagggccgct ggcgtttgcc
     6541 gagaagcgcg atccggtgtg gaaaggacgt taacaaccgt gagctattac gcctttgagg
     6601 gattaattcc ggtggttcac ccgacggcgt ttgtccatcc cagtgcagta ttgattggcg
     6661 atgtgattgt gggagtcggt gtctacatcg gcccactcgc ctcactgcgt ggtgactacg
     6721 ggcggttgat cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg catggctact
     6781 gcgacactga caccatcgtt ggggaaaacg gccatatcgg gcacggagcg atcctgcatg
     6841 gttgtgtgat tggtcgcgat gcactggtcg ggatgaacag cgtgattatg gatggcgcga
     6901 tcattggcga agagagcatt gttgccgcta tgagctttgt caaagcgggc tttcgcggcg
     6961 agaaacgcca gttgttgatg ggtacgccag cccgcgctgt acgcagcgtg agtgacgacg
     7021 agttacactg gaaacggttg aataccaaag agtataagga tcttgtcggg cgctgtcatg
     7081 catcattaca tgaaacgcag ccactgaggc aaatggagga aaatcgcccc cgtttgcagg
     7141 ggacgacgga tgtgacgccg aaacggtgat ctttccggat gcggcgtaaa cgccttatcc
     7201 gacctggctg gaaaggaacc gtaggccgta taagatgcgc cagcatcgca tccggcgacc
     7261 aggtcgcacg gtatcaacga aacgacatta acgacgcata ctctttgaca acatcatctg
     7321 ccacttctgc tcgcggttaa gttccgtgag cggaacggca ggaatacgtg gcgtctttct
     7381 tacgccgact aatttttccc gactgttatt gcgcaggcgc gcgtaaattt gctcatcgat
     7441 atcgaccact ttaaccagtc gctgacactg gcatccccgc ccttccagct tattagggat
     7501 catcttaact tcgcagctta tttctgtgac ttccgacaga atataagtca gggtattaac
     7561 cgccttgctg tgttcaatat cgaaatgaat agagatctct cttgctatca cccaccgatt
     7621 ttcagccatc atccattcgg cgattaacaa gtagagtggt ttttcaacat atccttcaca
     7681 catacgaact ccagtttcac aaattaaaag tctggctatg actcgttttg cagcaattaa
     7741 acaaggccat attaaaattg tggatatatc gatagtatgc aacttcaccg tcgataagtg
     7801 tgagctaaat cctgcttatg tcatcaaaaa tatcgattcc ccaaaagatt tacttaatgg
     7861 gcaaaaaaaa cggtcctcat cagagaaccg tattagttat tcgatcaaat tagcggatga
     7921 aaaatatccg ccatgacacg ctattatttg atctgcgcgt gcatttcctg caccgaaatt
     7981 actttttcag tcgcatcggc attcagcgcc atcgcggtag cgaaaccacc gttcagggtg
     8041 gtgtcgtaat gcactttata ttgcagcgca ctgcgacgga tcacgcggga gtcttcaatc
     8101 gcacgacgac ctgaggtggt gttgatgatg taggtatatt cgccattctt gatacggtcc
     8161 tgaatgtgcg gacgaccttc atgcaccttg ttcaccagac gcggattaat gcctgcttcg
     8221 cccagcacaa tcgccgtgcc gtgggtcgca tccagctcga agccctgttt cagcagtttt
     8281 gccgccaggt ccaccacgcg ttctttatcg ccttcgcgca cggaaagcaa cgcacgaccg
     8341 tgtttcttca tggtggagtt gctgcccagc tgcgctttgg cgaacgcttc agcgaaggtg
     8401 cggcccacgc ccatgacttc cccggtagag cgcatttctg gccctaacag cgggtcaacg
     8461 cccgggaatt tattgaacgg cagcaccact tctttcaccg agtagtacgg cgggataact
     8521 tctttggtta cgccctgctc agccagcgat ttgccagcca tcacacgcgc cgccactttt
     8581 gccagcggta cgccggtggc tttggagacg aacggaacgg tacgcgccgc acgcgggtta
     8641 acttcaatca ggtagacttc gttgtttttc accgcaaact gcacgttcat caggccgcgc
     8701 acctgcaatt cgaaggccag tttctgcacc tgctggcgca tcacatcctg aatttcctga
     8761 cttaaagtgt aggctggtag cgaacatgca gagtcaccgg agtgcacgcc cgcctgctcg
     8821 atgtgctcca tgatgccgcc aatcagcacc atttcaccgt cgcagatggc atccacgtca
     8881 acttctactg cgtcatcaag gaagtggtcc agcaacactg gcgcatcgtt agatacgctg
     8941 accgctgtct ggaagtagcg acgcaggtca gcttcgtcat agacgatttc catcgcccga
     9001 ccgccgagaa cgtaagacgg acgcaccacc agcgggtagc caatctcttt cgctttctca
     9061 accgccattt cgatggtggt aacggtggcg ttcgccggtt gtttcagttt cagacgctca
     9121 accgcatgct ggaagcgttc acggtcttcc gcacggtcga tggcatccgg gctggtaccg
     9181 ataaccggta cgccagcagc ttccagcgcg cgcgccagtt tcagcggggt ctgaccgccg
     9241 tactggacga taacgccttt cggcttctca atacgcacaa tttccagcac gtcttccaga
     9301 gttaccggct cgaagtagag acggtcagag gtgtcgtagt cggtggagac ggtttccggg
     9361 ttacagttaa ccataatggt ttcataacca tcttcgcgca gcgccagcga ggcgtgtaca
     9421 cagcagtagt cgaattcgat accctgaccg atacggttcg ggccgccgcc gagcaccatg
     9481 attttttcac ggtcggtaga cggattcgct tcgcactctt cttcataagt ggagtacatg
     9541 taagcggtgt cggtggcgaa ctctgccgca caggtatcca cgcgcttata aaccgggtgc
     9601 aagtcatact ggtcgcgcag cttgcggatt tctgcttcac gcacgcccgc gagttttgcc
     9661 agacgcgcgt cggcaaagcc tttgcgtttc agctggcgca ggaagtcagc gttcaggcca
     9721 gtgatgccca cttctgcgac tttctcttcc agacgcacca gctcttcaat ttgtaccagg
     9781 aaccagcggt caatgttggt caggttgaac acgccgtcta cggacaggcc cgcgcggaaa
     9841 gcatcggcga tataccagat acgctctgcg cctgcatctt tcagttcgcg gcgaattttg
     9901 gtcagcgctt ccggatcatc gaggctgact ttcgggtcga agccagttgc gcccacttcc
     9961 aggccgcgca gcgctttttg cagggattcc tgctgcgtgc gaccaatcgc catcacttcg
    10021 ccaaccgatt tcatctgagt ggtcagacgg tcgttcgccc cggcgaattt ctcgaagttg
    10081 aagcgaggaa ttttagtcac cacatagtcg atggacggct cgaaggaggc cggagtacgc
    10141 ccaccggtga tgtcgttcat cagctcgtcg agggtgtaac ccaccgccag tttcgccgcc
    10201 actttggcaa tcgggaaacc agtggctttc gacgccagcg cagaggagcg agacacgcgc
    10261 gggttcattt cgataacaat caggcgaccg tttttcgggt tcaccgcaaa ctggacgttg
    10321 gaaccaccgg tttcaacgcc gatttcacgc agtaccgcca tcgaggcgtt acgcatgatt
    10381 tgatattctt tgtcggtcag cgtttgggct ggcgcgacag tgatggagtc accggtgtgg
    10441 atgcccatcg catcaaagtt ttcgatagag cagacaatga tgcagttgtc gtttttatca
    10501 cgcaccactt ccatctcgta ctctttccag ccgatcagcg actcatcaat cagcagctct
    10561 ttagtcggag agagatccag accacgggcg caaatttctt caaactcttc gcggttataa
    10621 gcgataccgc cgccgctacc gcccatggta aaggatgggc gaataatgca cgggaagcca
    10681 acttcagcgg caaccgccag cgcttcttcc attgaatgag cgataccgga acgcgcggtt
    10741 tccagaccga ttttcttcat cgctacgtcg aaacggcggc ggtcttcggc tttatcaatc
    10801 gcatcggcag tggcaccaat catggtgacg ccgaactctt ccagcacgcc ctgacgctca
    10861 agctcaagcg cacagttcag cgccgtctgc ccccccatgg ttggcagcac cgcgtccgga
    10921 cgctcttttt caataatctt gcgtaccact tcccagtgaa tcggttcgat gtaggttgca
    10981 tcagccattt ccgggtcggt catgatggtc gccgggttgg agttcaccag aatgacgcgg
    11041 taaccctctt cacgcagggc tttacacgct tgcgcgccag agtagtcaaa ctcacacgcc
    11101 tgaccgataa caatcgggcc cgcacccaga atcaggatac tttttatatc tgtacgtttt
    11161 ggcatggctc ttttactcct gattacttag cggttttacg gtactgctca attaactcga
    11221 taaagtggtc aaacaacggc gcggcgtcgt gtggaccagg gctggcttcc gggtgtccct
    11281 ggaagctgaa cgccggttta tcggtacgat gaatgccctg taacgtaccg tcgaacaggg
    11341 atttatgtgt gacacgcagg tttgcaggta atgttgcttc atccaccgca aaaccgtggt
    11401 tctgggcggt aatcatcacc acatttttct caacatcttt aaccggatgg ttgccgccgt
    11461 ggtgaccaaa tttcattttt acagtcttcg caccgctcgc cagcgccagc agctgatgac
    11521 cgaggcagat gccgaatacc ggaatatcgg tttcgaggaa tttctggatg gcggtaatgg
    11581 cgtaatcgca cggggccggg tcgccaggac cgttggagag gaagatgccg tctggattca
    11641 ttttcagcac gtcttccgca gaagtttgcg ccggaacgat ggtcagacga cagcctctat
    11701 ccaccaacat gcgcaggatg ttgcgcttgg caccaaaatc ataagccaca acatggaacg
    11761 gcagctcgtc ttctttcttc gcttctggca ggccgccggt caacgtccag ctcccttgtg
    11821 tccagctata ggcttctgcg gtggtcactt cttttgccag atccatgccg ttcagacctg
    11881 ggaacgcgcg ggctttttct aatgccagcg ccgcatccgg gttatcgcct gcgataatgc
    11941 agccattctg tgcgcctttc tcgcgcagta aacgcgtcag cttacgggta tcgatatcgg
    12001 caatcgccac gatgttatgg cgcttcaggt aagaagagag gtcttcggta ttacggaagt
    12061 tgctggcaat cagcggcagg tcgcgaatca ccagaccttg tgcatgtacc tgagaagatt
    12121 cttcatcggc gtcattagtg ccgacattac caatatgggg ataagtaaga gtaacgattt
    12181 gacgagaata ggaaggatca gtgaggattt cttgataacc ggtcattgaa gtattgaaaa
    12241 cgacttcccc aaccgccgaa cctgttgccc ctatggcccg accgtgaaac tgggttccgt
    12301 cttccagaac caatagcgct gacttaatca aaacaccctc cagagaatat tcactcactt
    12361 tatttgcata ttaattcaca atgatgacgt aaatcaatgc aaatctgctt accgacgaat
    12421 ttctggcaaa cggcggcatt ctggagatat gggcgctaaa agtcaactta atggcgggat
    12481 tttttatatt attttgtggc tcatgcatgt tttacaggca ttaagcgaca aaaagatcat
    12541 ataacatgtt ctggaaaacg cttgcgcagc aagaagattg catgaaatca aaatgagcag
    12601 aaaaaagtgg accaaatggt caaaatttac attaaaacaa caaaaacaga atacaaaaca
    12661 tgtaattttc agcacttgca ttaaatttaa aagcataaca ccaaaaataa aagggctaaa
    12721 aattaaagcc ctttatattc aatgcattaa atgtgttatt tttgcatcac tataaatatt
    12781 ttgtggttac agattattga gatcaagcac atctcgcata tcaaaaagac cactttcctt
    12841 accactcaac cacaaagccg atcttaccgc accgttagca aatgtcatgc ggctggacgc
    12901 cttatgggtg atctccagac gctcgccaat atcggcaaac atcgcggtat gttcaccaac
    12961 gatgtcacct gcacgcacgg tggcaaaacc aatggtgcca ggcacacgtt caccggtatg
    13021 gccttcacga ctgtagaccg cgcaatcttt cagatcttta tccagagcgt gggcgatcgc
    13081 ctctcccatt gccagtgcgg tgcctgacgg cgcatcaact ttatgtctat gatgtgcttc
    13141 aataatttcg atatcggtgt agtcacccat cactttggct gctttctcca gcagtttaag
    13201 catgacgtta acgccaacgc taaaattggc ggcaaagaca atcgcaatat cggcggcggc
    13261 gtcacgaatc gcctgtttac cggcttcgtc aaaccccgta gtgccgatca ccatcccttt
    13321 gccatgctgg cgacaaaaag cgagatggtt cagcgtacct tccggacggg taaaatcgat
    13381 aaacacatca aaatcatctt ttatcgcatc aagactactt tgcacagtaa cgcctgtttt
    13441 cccggctccg gccagttcgc ccgcatcgct gcccagtaaa gaagatcctt cgcgctccag
    13501 cgccgcgccc agctgcacgc cttctaatgc cagcgccgcc tgaatcaact ggcggcccat
    13561 acggcccccg gctcccgcga tggcaacgcg gatgtttgca tcatgcatag ctattctctt
    13621 ttgttaattt gcatagaccg ttttcagagt aaccagcgca tcgctacgcc gccagccaaa
    13681 aacgctgata attagaaatt aatgataaac agggagggat atcagtgaaa ggcatgagtc
    13741 gatgaatgac tgcatgccga taacatgtga caggttacgg caccagagcc agcacctcag
    13801 ctacccactg ctggaagcct tttacattca gatccaatgc cacctgtaca ttggctggtt
    13861 tgcccaggca accgtcgata tcaaccaccg tcgtgccaga ggtaaattcg ccctgcgttt
    13921 ccactgccac aaaacacggc ttgagagtga acaggtccgg gcgcaccagc caggcgatgg
    13981 cgcagagatc gtgcattcgc agtccacttt gcatactgcc gctacggtag tgactaaaca
    14041 gggcgtggag catttttccg gtgcgattca actccggcaa ggtagcgaga tagtcagggg
    14101 ttaatattgc ctgattggtg acatccagac cgcacatgac gatttcgatg ccgctacgga
    14161 agacacaggc tgccgcttct ggatcggcag cgatattaaa ctcggcgttt ggtgtgcagt
    14221 tgccgcgacc agcagaaccg cccatgatca ccagacggcg aatatacggc ttacattccg
    14281 ggcattgtga aagtaacagc gcaatattgg ttaacgggcc gatggccacc agggtaacgg
    14341 gctctggcgc acgcatcagg gcatcacgaa ttgccagaaa cgccggtata cccagtggct
    14401 gacggttgtg ttcaacaaag tcatagccag ccattcccga ttcaccgtgt acagatgccg
    14461 catcgcgcgg cgcacgaacc agtggtacgg cagcgccctg ggcaagcgga atctccgcat
    14521 tccagaaatg cagcagttgc agggcgttgc gggtcgtttt ctctaccgaa acattacccg
    14581 cgacagtggt catcagttgc agatcgagtt cgggggtaaa aatcgcggcg gcaatggcaa
    14641 cggcatcgtc aatgccgggg tctgtatcga ggaagatagg taaacgcatg ttttctccat
    14701 aaaaaatgcc ggtaacaaga ccggcatttt cgcataactt aggctgctaa tgacttaatc
    14761 gacttcacga atatcgacac gcagctcttt cggcacttcg aaaacaatgt tttcttcacg
    14821 accttccagc ggaatggctt caccaccgcc cagctgttgc aaacgtgcca ccacattctg
    14881 caccagaata tccggagccg atgcgcccgc agtcacgcca acgcagttca cgccttttac
    14941 ccactcttcc tggatatctt tcgcatcgtc aatcaaaaat gcgcgtttgc ccatacgctg
    15001 ggccagttcc gccagacggt tggagttgga ggagtttttc gaaccaacca ccagtactac
    15061 ttccgcctgc tctgccaggg cgcgtaccgc ctcctgacgg ttagtcgtgg cgtagcagat
    15121 gtcatctttg cgcggaccga caattttcgg gaagcgttta cgcagcgcgt cgatcacatc
    15181 agacgtgtca tccaccgaca gcgtggtttg ggtcataaag gagagcttct cttcgttttt
    15241 gaccgtcagt ttccacacat cgtctggcga ttcgaccaga tacattcccc cttccgggtt
    15301 gctgtactgg cccatcgtcc cttccacttc cgggtgcccg gcgtgaccga tgagaataga
    15361 ttcttcacca cgacggctgg cgcgggcgac ttccatatgc actttggtca ccagcggaca
    15421 ggtggcatca aacaccgtca aatcgcggct tttcgcttcg ttacgtactg cctgagaaac
    15481 accgtgtgcg gagaaaatca ggatcgcgcc gtccggtact tcgctaatct gctcaataaa
    15541 gatagcccca cgctcgcgca agctatcgac cacgtagcgg ttatgcacca cttcgtgacg
    15601 gacatatatc ggtgcgccgt aaatggccag cgcgttttca acaatgctga tagcgcggtc
    15661 taccccggca caaaaaccac gcgggttggc caacaggatc tgcatgttac gcctccagtg
    15721 ccggatcgat ttccagcact tcaatatcaa aatgaacggt ctgcccggcc agcggatggt
    15781 tgaaatcaac ggtaatggag tcgccgttaa tttcgcggat cacgccaggc atctcactgc
    15841 catccattgc ggtaaaaagc atgattgcgc caatttctgg ctcgcctgca tccataaatt
    15901 cacggcggga gaagtactga atcaggtccg gtgacggcac gccaaacgcc gcatcaggct
    15961 ccagcgagaa ggtggtttta tcgcccactt tcagccccag caggtgttgc tccagccctt
    16021 cagaaagaga agcatcaccc aggcggaaca gcgccggttt accgttgttg cgggtagact
    16081 cagcggtggt gccatcatcg agtttcagcg tgaagtgcac caggacggcg ctattgctct
    16141 gtacagattc agacatgcag gtttgctctt atattttgag gatttttgcc ggatgcggcc
    16201 tacgatttcg cagcaatctg taggccggat aagatgcgtc agcatcgcat ccggcagggt
    16261 ttattattgt tttttcgctt tagaaggtaa aaaaccttcc agcacaatca gtgccgcacc
    16321 gacacaaatg gcagtatcgg caaggttgaa ggtggcgaag tgccagtcgc cgacatagaa
    16381 gtcgatcata tcgacaacga agccgtgcca caggcggtcg aacaggttgc ccagcgcgcc
    16441 gccaataatc agcgcgtaag cgatattgtt tagcttctgc gtggccttcg agcgatacat
    16501 catcactgcc aggatcacgc taataccaat cgcaataccg gcaaaaaacc aacgctgcca
    16561 gccgccacta tcggcaagga aactaaacgc cgcgccatag ttacgcgcat aatgcagatt
    16621 aagcgacggg aacagcggga ccgtatcccc cagagcaaag ttctggagga tcaggtattt
    16681 gctgcccaga tcgataatca gcacgactac caccagccac agccagcgta gccctgttga
    16741 acagatcgat tgactcatca ggcaaactta cgtttttcac cgtcaccggc gacgttgctg
    16801 acacagcggc cgcagatttc tgcgtgttcc gccaccttgc cgacatcctg ggtgtagtgc
    16861 cagcagcgtg ggcacttctc accttcggct ttactcaacg cgactttcag ccctttgagg
    16921 acttcgctct gctgggcgtc agcaggtgcg tcgttatagt ctgcaacggt agcgccggag
    16981 gtcaacagga caaatcgtaa ttcatcgccc agcgcggtca gtttcgccgc cagttccggt
    17041 tctgcataca aggttactgc cgcttccagc gagccgccca ctttcttatc ggcacgcgct
    17101 tgctcaatga ctttgttcac ttcgccacgc actttcaaca gctcgtccca gaacgcatcg
    17161 ttcattgctt cactgtctgc cagaccaaac aggccttcgt accactcacc ggtgaagacg
    17221 tatttttcac gttcgcccgg caggtaaccc cacacttcat cagcggtgaa ggagaggatt
    17281 ggtgccatcc agcgaaccag cgcttctgcg atgtgataca gcgcagtctg gcagctacga
    17341 cgcgccacgc tgtccgcttt ggcggtatac tgacggtctt tgatgatgtc gaggtagaag
    17401 gaccccatct caacggagca gaagcgcatc agacgctgta ccacttcgtg gaaatcgtat
    17461 gcttcgtacg ctttgaggat gtcttcctgt gccgcgttcg cacaacctac ggcccagcga
    17521 tccagtacca ccatctcttc cggtttcacc atatcttttg ccggatcaaa accgttcagg
    17581 tttgccagca ggaagcgcgc ggtgttacgg atacgacgat agctgtcggc agcacgtttc
    17641 aggatctcgt cagaaacggc catttcgcca gtgtagtcgg ttgatgccac ccacagacgc
    17701 agaatatccg cacccagttt gttcatcaca tcctgcggcg aaacggtgtt accgatggat
    17761 ttagacatct tgcgaccctg accatccacg gtaaagccgt gagtcagtac ctgacgatat
    17821 ggcgctttgc ccttcatcgc ggtagagatc atcagggaag acatgaacca gccacggtgt
    17881 tggtcagaac cttccagata catgtccgct gcgtgaccgg caaattccgg acgcacatca
    17941 acaacggaag agtgggtaga tccggagtca aaccatacat ccagcgtatc cggtactttc
    18001 acatactggt cagcttcgtc gccgaggatc tctttcgcat cgagatccca ccacgcctga
    18061 atgccgtcaa cttcaacgcg ttttgccact tcttccatca gttcgagagt acgcggatgc
    18121 agttcttctg tgtctttgtg cacgaacagt gacatcggca cgccccaggt acgctgacga
    18181 gagatacacc agtcaggacg gttagcaacc atcgactcga tacgcgcctg gccccagtca
    18241 gggatccact gcacgccttt gatctctttc agtgactgcg cacgcagacc tttctgatcc
    18301 atgctgacga accactgcgg cgtcgcgcgg aagatgatcg gcgttttatg acgccagcag
    18361 cacggatagc tgtgttgcat tttctcaacg tgcaacagtg cgcctttttc ctgcaacaac
    18421 gcaatgacaa tatcgttcgc tttgaagacg ttaacgccat ccagagtcgg gtaagtaccc
    18481 ggcagataag tgccgtccgg gccaaccggg ttagcggttt ccagaccata tttttgaccg
    18541 atcacatagt cgtccggacc gtggcctggc gcggtatgaa cggcaccggt accggcatcc
    18601 agcgtaacgt ggtcgccgag aattgccgga acatcgaagt ccataaacgg atgggtaaag
    18661 cgcaacagtt ccagctcggc acctttcacc gtgccaagaa tggtgtaatc gctaacgccg
    18721 atacgctgca ttacgctttc aaccagatct ttcgcgagga tcacggcctg accgtcgatt
    18781 tgcaccagcg cataatcaaa atcaggtgcg atggagattg cgcggttagc aggcagcgtc
    18841 cacggcgtgg tggtccatat aaccagcgaa attgggccgt taacattgct tacgccaaat
    18901 ttcgttttaa gcgcatcctg atcgaccgcc tggaaagcga cgtcgatgga cagagaagtt
    18961 ttgtcgtaat actcaacttc cgcttctgcc agtgcagaac ggcagtcaac gcaccagtgc
    19021 accggcttcg cgcctttgtg caggtgaccg ttgccgatga ttttgcccag cgcgcggatg
    19081 atgttggctt cagttttgaa gtccatggtc aggtacgggt gcgaccagtc gcccagcacg
    19141 cccagacgga taaagtcttt gcgctgaccg tcaacctggg tcgcagcgta ttcgcggcac
    19201 ttggcgcgga actcagcggc ggtgaatttc tcccccggct taccgtattc ttgctctact
    19261 ttcagctcga ttggcagacc atgacagtcc cagccaggca catacggcga gtcatatcca
    19321 gaaagccctt tggacttaat gataatgtct ttcagaatct tgttaaccga gtgaccaata
    19381 tgaatactgc cattcgcata aggagggcca tcatgcagaa tgaaggtttt tttgcctttt
    19441 ttagctgcac gaatgatgcc gtacagatca tcatcagtcc aacgcgccag cattcccggt
    19501 tcgcgcttgg cgagatcgcc acgcatcggg aaccctgttt ccggcaaatt cagggttgat
    19561 ttatagtcac tcatcagatt ctcggttccg tatttcggtt tgattacata acaggcttaa
    19621 gccggttttg ttagcccaaa aaattcgcgg gcggttaatt catcacgcgc aatctgcgct
    19681 ttcagttcgt ccagcgatgc aaatcgctgc tcattgcgta tttttttacg cagcactact
    19741 tgtatatggc gaccataaag gtccattgca acatctaaca aatgcacttc cagttgctgg
    19801 cgaataccgg caaccgttgg gcgtgttccg atgtttgcaa cgccgggtaa cggcttttcg
    19861 ccaaggccca acacttctac cgcataaacc cctttcaccg gggaaacctg acggcgtagc
    19921 ggtacattcg ccgtcgggaa acctatagtg cgccctaatt catcaccgtg gactacacgc
    19981 ccggagatag caaacgggtg ccccagtaaa ctttctgcca gagccagatt gtcatccgca
    20041 agcgcctgac gcacggcggt gctgctgata cgcacgccac cttcgcaaaa agtttgcgtg
    20101 ctggtgatat cgaagccgta ttccatgccc gctttctgta ataacaagaa atcgccttca
    20161 cgaccagcgc caaagcggaa atcgtcacct acggcaagaa attttacccg caagtgcttc
    20221 accagaagat cactgatgaa gttttgcgcg gttaacgccg caaaacgcct gtcaaaacgc
    20281 acgcacagca cgtaatcaac accacactct gcaagataac gcagtttttc ccgcagccgg
    20341 gtgagccgtg ccggggcttt atcagtagca aacagttcca gtggttgagg ttcaaaaagc
    20401 atcaccatca ccggtaagtt gcgcttgcgc ccttcttcct gcaagccctg taacagcgcg
    20461 cgatgaccgc gatgcacgcc gtcgaaatta ccaatagtca gcacacaccc ttcttgcggg
    20521 gcctggctga gattatgtat gccgcgtatc agcttcatgt ctggctcaaa acagtgaaaa
    20581 tcgtccgagt ataccttgta cagcggtcaa ggttaaccgg cgattgagta ccgaaatcag
    20641 aaagaagtgg cttttcattg ctatggcgca aatcatggga agaaactgac cgcctgctgc
    20701 aatttttctc gcggaaaagc tgtattcaca ccccgcaagc tggtagaatc ctgcgccatc
    20761 actacgtaac gagtgccggc acattaacgg cgcttatttg cacaaatcca ttgacaaaag
    20821 aaggctaaaa gggcatattc ctcggccttt gaattgtcca tatagaacac atttgggagt
    20881 tggaccttgg ctaatatcaa atcagctaag aagcgcgcca ttcagtctga aaaggctcgt
    20941 aagcacaacg caagccgtcg ctctatgatg cgtactttca tcaagaaagt atacgcagct
    21001 atcgaagctg gcgacaaagc tgctgcacag aaagcattta acgaaatgca accaatcgtg
    21061 gaccgtcagg ctgctaaagg tctgatccac aaaaacaaag ctgcacgtca taaggctaac
    21121 ctgactgcac agatcaacaa actggcttaa ttgcctgttg ttgtcagctt tgtaaaaaaa
    21181 cccgctctgg cgggtttttt tatatccatc tgcaggccaa aattcggcct gcaaagtaca
    21241 aatgtttatt ttgaagctgg agtaaacagc gcagaatagt ctgtattgca gatacgctgt
    21301 actgcagggt gctgaatcat cctttcggca aaaatcgcgt ggtactcttc catcacgttc
    21361 tcaacacggc ctatctccac aaccgagtca tcgttataga aatcattagc gtaaagcgaa
    21421 ggtgcaacga aaatagcgtt atgcgtcgcc ccaaaggctt tcatcaacgc agcatcatca
    21481 aactcaccca aaatttcgac gttcaagccc tgggagttaa accagtttaa tagcttacgc
    21541 cccaacattg agcgacgccc cggaataagt aaacgacgct cttcaagaca ggcaggaaac
    21601 ggcttttctg gtagtgggtt agtgcaccag aaactgacac cacattcgcc aattttcatg
    21661 gaaaacagcc cttcctgctg agtggaatcg atcggacagt cagagatgat catatccagt
    21721 ttatgctgac tcaactgctc taaaagcatc tcgtgcgtcg attcgaaaca gcgtagatgg
    21781 atctgctcgt cttccacaac tgcggcatcc agaacactgc tgaccagacg tttggaaagt
    21841 gcatctgcca caccaacatc aaacaataag ttggactctt tgcgatagtt gacgatatcc
    21901 agcatttcct ggcttaaggt gaacatttta tcggcatagc gatagaccag ctctcccagc
    21961 tcgctgggct cgaggccacg tcccttgcgc ttaaataatt tgccttgcag gcgctcttcc
    22021 agcgcccgaa tctgtccggt aatggtttgt ggtgttaaat aaagcgcctc cgctgcgcca
    22081 accacggaac cttctttata gacatgccag aagtaataca agtggttgta attgatatga
    22141 gacatgctca tttctctccc tgataacaat gaaaagggag ccgtttatgg ctccccggta
    22201 aaccgtcctg tcaaactgat ggacgcaaac gaacgcgtaa ccagctgtat ccaattaccg
    22261 ccgaagagat agaaccgacc aggataccga gtttcgccca gttaatcagt tctggatcta
    22321 cgctaccaaa ggccaggctg gcaataaaga tagacatagt aaaaccgata ccgcacagga
    22381 tccccaccgc cataatttgc tgataagtcg ttccctcagg cagatgcgcc agtttcaaac
    22441 gcagcgccaa ccagcagaac agactaatcc ccagcggttt gccaatcagc aagccagcga
    22501 tgatccccaa tggcagaatg gaggtcaagc catccagcgt gacgccttgc agtgaaacgc
    22561 cagcattagc aaatgcaaac agcggtaaaa tcagatacgc cacccacggg tgcaacacat
    22621 gctccagtcg cttcgctgga gaacgcccat gcttctcttt caaaggaata aagaagccga
    22681 caattacccc cgccagggtt gcgtgaaccc ccgatttcaa caccgcagtc cacaacacca
    22741 cgccaaccag aatatagacg cccgtgcggc gtacaccaca cagattcaat accgcgagta
    22801 ccgcaattgc tacagccgcg acgccaagag aggccatcga taagtcatta gtgtagaaca
    22861 atgcgatgat aatgatggcc ccaagatcgt cgataatagc cagagccatc aaaaagatct
    22921 tcagggctaa cggaacacga cttcccaaca gcgccagcac accaagtgca aaagcaatgt
    22981 cagtagccgc cgggatcgcc cacccttcgc gggtaatcgg atcggcatgg ttaaaggcca
    23041 gatagagtaa tgccggcaca atcatcccac caatagcggc gataactgga aatgcagcct
    23101 ggcgtaagct ggctagcgat ccttgcatca gttcacgttt aacttccaga ccgactaaca
    23161 ggaaaaatac cgccatcagc gcgtcattta tccataacag catgtttttg ttgatttcga
    23221 gtgaaccaac ccggagctga accggcgtct ccagaaagtc gtgataccat ccactggttg
    23281 cgccgctgtt ggccataatc atcgccagga tagcggcaat gataagaata atgcctcccg
    23341 aggcatcact gctaaagaat cgatgcagat gtttcacttt ttatttctct ttcaggtgaa
    23401 tagatcgttt ttactatctt accccgcacg aatcatcgtt aaaatcagat tatagtgtag
    23461 gaataattcg ctttttacga ggcattgaag gatttgcgtc atcagtgatg agatgagttt
    23521 ttgacggctg taagcgctga cgtgtgaccc taagccctga taatcatagc ttaaggtcag
    23581 gatttctgag ggattaatta ggaaaagtaa taattaattt gttgtcagtc ggcgatgttt
    23641 gttaggccaa tacttcaaaa ataatccagt ttgtcgtcaa t
//
LOCUS       DAZKGF010000073        23391 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZKGF010000073 DAZKGF010000000
VERSION     DAZKGF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.073"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..331
                     /locus_tag="ACHOQV_002648"
     CDS             <1..331
                     /locus_tag="ACHOQV_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9631006.1"
                     /translation="EKITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAK
                     WSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFP
                     QPVSLAG"
     gene            complement(374..1525)
                     /gene="ydiO"
                     /locus_tag="ACHOQV_002649"
     CDS             complement(374..1525)
                     /gene="ydiO"
                     /locus_tag="ACHOQV_002649"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFP9631007.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1539..3134)
                     /gene="ydiF"
                     /locus_tag="ACHOQV_002650"
     CDS             complement(1539..3134)
                     /gene="ydiF"
                     /locus_tag="ACHOQV_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFP9631008.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3280..4038)
                     /gene="aroD"
                     /locus_tag="ACHOQV_002651"
     CDS             complement(3280..4038)
                     /gene="aroD"
                     /locus_tag="ACHOQV_002651"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP9631009.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVIAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDIIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4069..4935)
                     /gene="ydiB"
                     /locus_tag="ACHOQV_002652"
     CDS             complement(4069..4935)
                     /gene="ydiB"
                     /locus_tag="ACHOQV_002652"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFP9631010.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4947..6212)
                     /gene="ydiN"
                     /locus_tag="ACHOQV_002653"
     CDS             complement(4947..6212)
                     /gene="ydiN"
                     /locus_tag="ACHOQV_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9631011.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFATFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(6439..7653)
                     /gene="ydiM"
                     /locus_tag="ACHOQV_002654"
     CDS             complement(6439..7653)
                     /gene="ydiM"
                     /locus_tag="ACHOQV_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9631012.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALLLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHSLKENVAPGG"
     gene            complement(7753..8109)
                     /gene="ydiL"
                     /locus_tag="ACHOQV_002655"
     CDS             complement(7753..8109)
                     /gene="ydiL"
                     /locus_tag="ACHOQV_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFP9631013.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTDDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTVFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8245..8352)
                     /gene="rprA"
                     /locus_tag="ACHOQV_002656"
     ncRNA           complement(8245..8352)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHOQV_002656"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8538..9650)
                     /gene="ydiK"
                     /locus_tag="ACHOQV_002657"
     CDS             complement(8538..9650)
                     /gene="ydiK"
                     /locus_tag="ACHOQV_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFP9631014.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            10037..13093
                     /gene="ydiJ"
                     /locus_tag="ACHOQV_002658"
     CDS             10037..13093
                     /gene="ydiJ"
                     /locus_tag="ACHOQV_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFP9631015.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDETLIDERVNALCARLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSSMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALDSQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWVAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13090..13500
                     /gene="menI"
                     /locus_tag="ACHOQV_002659"
     CDS             13090..13500
                     /gene="menI"
                     /locus_tag="ACHOQV_002659"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFP9631016.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13600..13788
                     /gene="ydiH"
                     /locus_tag="ACHOQV_002660"
     CDS             13600..13788
                     /gene="ydiH"
                     /locus_tag="ACHOQV_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFP9631017.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14337..14705
                     /gene="sufA"
                     /locus_tag="ACHOQV_002661"
     CDS             14337..14705
                     /gene="sufA"
                     /locus_tag="ACHOQV_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFP9631018.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14714..16201
                     /gene="sufB"
                     /locus_tag="ACHOQV_002662"
     CDS             14714..16201
                     /gene="sufB"
                     /locus_tag="ACHOQV_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP9631019.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16211..16957
                     /gene="sufC"
                     /locus_tag="ACHOQV_002663"
     CDS             16211..16957
                     /gene="sufC"
                     /locus_tag="ACHOQV_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP9631020.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16932..18203
                     /gene="sufD"
                     /locus_tag="ACHOQV_002664"
     CDS             16932..18203
                     /gene="sufD"
                     /locus_tag="ACHOQV_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP9631021.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18200..19420
                     /gene="sufS"
                     /locus_tag="ACHOQV_002665"
     CDS             18200..19420
                     /gene="sufS"
                     /locus_tag="ACHOQV_002665"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFP9631022.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19433..19849
                     /gene="sufE"
                     /locus_tag="ACHOQV_002666"
     CDS             19433..19849
                     /gene="sufE"
                     /locus_tag="ACHOQV_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFP9631023.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19998..21002
                     /gene="ldtE"
                     /locus_tag="ACHOQV_002667"
     CDS             19998..21002
                     /gene="ldtE"
                     /locus_tag="ACHOQV_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFP9631024.1"
                     /translation="MKCASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYNYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKGVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21066..21302)
                     /gene="lpp"
                     /locus_tag="ACHOQV_002668"
     CDS             complement(21066..21302)
                     /gene="lpp"
                     /locus_tag="ACHOQV_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFP9631025.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(21613..23025)
                     /gene="pykF"
                     /locus_tag="ACHOQV_002669"
     CDS             complement(21613..23025)
                     /gene="pykF"
                     /locus_tag="ACHOQV_002669"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFP9631026.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(23033..23227)
                     /locus_tag="ACHOQV_002670"
     CDS             complement(23033..23227)
                     /locus_tag="ACHOQV_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631027.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT         6069 a         5648 c         5892 g         5782 t
ORIGIN      
        1 cgaaaaaatc actttagagc agttatcgaa aaagtttcgt gccagcgtca gttatatttg
       61 ccatgaattt accaaagagt atcgcatttc ccctattaac tatgttattc aacggcgtat
      121 gacggaagcg aaatggtcac tcactaatac tgaattatca caggcagaga tctcctggcg
      181 tgtgggttat gaaaatgtcg atcactttgc caaactgttt ttgcgccatg tcggctgttc
      241 gcccagcgat taccgcaggc aatttaaaaa ctgttttgcg gaacaagaaa tcctatctga
      301 atttcctcaa ccagtaagtc ttgccggata aatctggcgg cgcagtttgt taagaagctg
      361 cgccgcctgc agattatttg ttctgataat ctttcaggat ctgccgacct gctacgtaaa
      421 tcataatttc gtctgtaccg ccgccgatac gttcacaacg gacatcacgc cagaagcggg
      481 agacgcgcgc ctcatcggta tagcccagac cgcccatgat ttgaatcgca tcatcaatga
      541 cttccattgc ggtacgtgcg cagtacagtt ttgccagcgc cgcgctggtg cgcagtgact
      601 gatgctgatc ggcttgccat gccactttca acaccatgtt gcgcatgttg tcgatcttaa
      661 tcgccatcag cgccagtttt tcctggatca tctggttatg accaatgggc ttaccaaaag
      721 cgatacgctg gttggcatag cgggcggcat cttcgaatgc gcattcggca aagccggtgc
      781 tgcgcgcggc gttgatcagg cgctccatct caaagttgta catcacattg aggaaaccca
      841 ttccttcttc gcccaccatg tcgctctctt caacttccac gttgtcgaga tagacttcgc
      901 aggtgctgag catatgccag ccgattttat gcagtggatt gattttaatg ccgggcttac
      961 tggagtcgac ccaccacaga gtaaaggcct ttttcggatc tttcggttgc ggatcgcgcg
     1021 ccaacaccag catatatgga tactctttcg caccggtaat aaaggttttc tgtccgttga
     1081 tgtaaacctt gccgttttta cgcgtgtaag tggtagtggc actgttgttg tctgagcctg
     1141 cgcctggctc tgtcaatgcc agggcatagg cgggatcacc cgtttccagg gtgctttccg
     1201 ccgttttacg tagctgctct gcagaaccga aacggcgcat actgtgaata cattgaccgt
     1261 tggtgatcaa aaatgccggg gcaccgcatt ttgacacttc catcagcgcc agcatttggg
     1321 tgacgtaatc cgcagggata ccaccaaatt cttccggcac gccaagcatg gaaataccgt
     1381 tatccgccag tgcccgcata aattcacgcg ggtatgtccc gttttgatcg caggtgcgga
     1441 aatactcttc cggaaagtta gtcgtaatca gttcgcgaat actggccagc agcagttctt
     1501 gttcttcagt taaagaaaaa tccatcatta tactcctgtt aatgagccgc ttcaggcagg
     1561 acaaaaccca tcgccgcatc gataaataat ctttcgtcca tcagtttgag ttctggcgaa
     1621 atcactgggg tgaaatccat tttgtcgaga atatcttttt gtaaatcgac gccaggagcg
     1681 atttcgatta aatgcaggcc gtcttctttc agcgtgaata cggcgcgctc agtgatataa
     1741 cgaacatcca gccctcgctc gagcgcgatt tttccgctaa aagtaatttc tggtagttcc
     1801 cgaataaatt tcttcacccg tccttcctgg acgatattta atttgccgtc ggtaatttct
     1861 gtttttaaac tgcccgcagt taatgtgccg cagaaaacga ttttcttcga agtggcactg
     1921 atatcaataa atccaccggt gcccatgatt ttaccattga atttatgcac gccgacgtta
     1981 ccgtgctggt cgacttcagc aaaactcaaa taacaaacat ccagaccacc accgtgataa
     2041 aaatcaaact gggacgtcat atccagaatg gcgcgggtat tcacgttcgc gccaaaggcg
     2101 atcccctgtg aagtaatacc gccaatcgga cctgtttcta ccgtcagaat aaagtcatca
     2161 gcacaacctt cttcccgggc gaccaggcca atgccgtcag caataccgac gccgacattc
     2221 cccactgcgc ctttgcgcat ttcgaataat gcacgccgtg caactaattt acgttggttt
     2281 aggggcaggc taagtttggt gctgtcatca agggtgaagt caccagaaat aaagcggtta
     2341 accggcgctc cgccatacag ttgggtttga tccggatcga ccaccacaat atccaccaga
     2401 taacccggaa tacggacaga tttaggatgc agcgtggctt tcttaaccat tttctgcacc
     2461 tgcatcatca caataccgcc gttattgtgc accgcctgag caataaccaa tgcgtcgaga
     2521 tacatcacct catcttcaaa agtggcgtag ccttcgctgt cgcaggtggt agcgcgaatg
     2581 aaggcgatat ctggcgctat cgctttgtaa tagagatatt ctttgttatc aaactcgacc
     2641 agtttaatca ggtcttcttt agtgacttca ttcagtttgc cgccttgctg gcgtggatcg
     2701 acaaatgttc cgatgccaat atcactaata ataccaggct ggtgggctgc ggcggcgcgt
     2761 aaggtttgtg taagtacacc ttgtggatag ttataagcaa taattttatt ttgttctgcg
     2821 agatcagaaa tacgcggcga ttgtccccag tgaccgcata atgcccattt caccaggcct
     2881 tcttgcgcca gcggactaat accacggtcg gcgcgatcgc caagccctgt tggactaata
     2941 atcgataaat tacgtggtgt ttgagtctgt ttatatttat cagcaagagc agtaattaac
     3001 gtggtggctt ccagaatacc gccgccagcg cctaacacac aaagtgttgc ttcgtcggga
     3061 atataattca ccgcttcctg tgccgacagg accggcactc gtccattaat gcgaggtggt
     3121 tttacaggtt tcattgttaa ttcctgattc aggtgtgatt ttccctacgc cagatggaga
     3181 attagcgcac agagactcac gaaatatttt tattaaatac ggttaaaaat acttttttta
     3241 gttcggcggg ggagagtatt cccgccgaaa tattattgct tatgcctgat gtaaaatagt
     3301 taataccgta cgcaaatcat ttaccgagat ttgccctggc gcagaggctt tttttaccgc
     3361 accaaaagtt gccgccgagc caaatacttc accagccaga cgagaaatta cgccagtttt
     3421 tgccatcgac atcgtgatga ttggacgatc ggcatactgc tcctgcatct ccagggtcgc
     3481 agcaagcaac gtcagcacat cgctggtact ttgcggcatc agcgcaatct taggaatatc
     3541 ggcatcgaag gactgcattt tgcgcagacg ggcaatgatt tcttcggctt ccggcgtttt
     3601 atggaagtca tggttggaca tgacaacttt cacatcatgc gcgtgggcgt aggcgacggt
     3661 ttctttaacc tgatcatcgc cggtaaataa ctccagatcg attatatcaa ccaggccgct
     3721 gtcgatggct gcacgattga gtgcaatata agcctcggtg gaaatcgcct gctcgccgcc
     3781 ttctttggca ctgcggaagg taaacagcag cggtttttct ggcatggttt cacggagaat
     3841 ttttgctgcc gctatgacag actccacatt ggagagatcg gcatagtggt ccacacgcca
     3901 ttccagaata tcaaagtccg cttcacgata ggcgagagct tcggatttca cgctggcgat
     3961 atctttcgcc atcagcgaga cgatgatttt aggtgcgccc gtaccaatga cgagatcttt
     4021 tacagttacg gttttcattt tttacccttt ctgcacgcgg tcagcctgtc aggcaccgaa
     4081 ccccatgacc tgtttaacat attccagagg gaaatctttg ccagtccata acgtgaactg
     4141 ttcagcccct tgccacaaca acatgccgta tccatcaatc gttttgcaac ccgcttgttg
     4201 cgcctgctgc aataacttcg tcatatgagg gttatacacg cattcagtga ccagaagtcc
     4261 cggatgtaac agactgatat cattaaccaa tgattcattc tcaaggggtt tcatacccac
     4321 tttggtgcca ttggttaaaa tgtcggcgga agccagggct tctgcaaagg cttgctgatc
     4381 ggcgagatcg gtgactgtca cgacgcaatc ggtgttttcg ttaacccgct gcgcgaaggc
     4441 gagagcttta tcgaagaact catcccgacg gttaaagagt ttaatttctt ttaaaccttc
     4501 aattgccccc tgcgcgccaa ttgccgttga ggcaccaccg gcccctaata gcaccatcgt
     4561 tttgcctttg atatcaaaac cgctctcttt aatggcgcga atatgacccg tgccgtcggt
     4621 gttatagcca cgcagatagc catcatcatt aacgatggtg ttgatggctc ccaccagttt
     4681 ggccgctggc gttaattcat caacatattc acacgccagt tgtttgttcg gcattgatac
     4741 accagttccg cgcattttga gggcttttaa tccttcaatt gctccgggaa aactatcgtt
     4801 atccacttca aaggccatat aggtaaatgg caatcccgct ttttctaagg ctttattctg
     4861 catttcgggc gataaactgt ggcggatggg ataggccatc aacccaatca attcgtattt
     4921 tgcggtaaca tccataatta actcctttaa cctctatgct taatcgtgtt taaccgggtg
     4981 ctaaatttat gctcgccaaa ccgcacatca ttttcaggaa taatgaaaac gcggtaatag
     5041 cggataaaaa caataattgc ggtaatcagg gccagcagcg cgaaagtaaa atcgagaaca
     5101 atgatatatt gcaggccgat gttcgacaga taaccggtaa tcagcggaat aacaaagtta
     5161 gccagtccac ccatcatcat atatatactg gtgactttgg ctttgctttt tgggaaaaac
     5221 tctgacatga ccgaaacgcc gagctgtaaa atgccgccag ctgctgagaa accgataaca
     5281 aaggctccgg cattacacac caatggagaa gggtacaggt agataatggc agctgttatt
     5341 gttgccagtg cagagttaaa tacattagcc cagatgggcc ggaccatttt tttcagcagg
     5401 gcggcaaaaa taaagacaca gaccagcgaa cccatactgt aataagagat ggtttttaat
     5461 gcctcagctt ctgacatacc agcaaaagcc atcgcatatt tgggcatcca caccacaatc
     5521 acataaaagg tcgcgaatgc ggctacaccg aacagtactg atgaaacacc ttccagccag
     5581 actaacggtt tgctgttcat ttgcggtaat tcattagcta cgctggcgtc caccaactgg
     5641 ctggggaatt tgcttttcaa cagcatcagc gtgatcagta caaacagaat acccggaata
     5701 atcagcccat agccgtacca gatgttattg agcaacatat agctcaccag cattgggtag
     5761 aacatttgcc cgaatgacac catcgcttta accagtatga ccgccgaacc agaggctttc
     5821 ggaaagcatt ccataagcgc ggggtagcca cccgtatcca gcgctgagtt agcgatacct
     5881 acgcacactg ccagacagta ggcgagagtt aaattcgggc aagcaggaat accaaagaag
     5941 aatagcagat acattattac tgccattaat atcaccgccc gacgaccaaa cttatcggag
     6001 atcacaccga agaataaaat actgatcaat cgccccaatc cgataccgga aattaaatag
     6061 gcaatgcccg cgttgtcagt ggaaaacttt tctgccaaag atgtcatgtt ttgggcaagc
     6121 gtaataacac taatgccgtg caggaaatag ctgaagtaaa tacaaagaac agccaggata
     6181 aatggcgtgc tgaaagcctt attttgagac attttcacag ctccttgctg aaagataatt
     6241 tcgttaccgt acgtattgtt ctccgacggt ttacataatt actatactga agataatttt
     6301 ccctgggtac atggcataaa gtttatgttt ccaggaatat tgcatgtcat agtttgtttt
     6361 tgatcgcgat tttccttagt tagcgcagaa agagttattt atccgtaaga tcttgcatga
     6421 cctgaaaaga atattgcatt acccacccgg agcgacattt tcctttagcg agtgatgccg
     6481 cgtttctttg cgactgcgta agccaataaa cagtgccagt ataaagccga tggcagcgat
     6541 ggcggtatcg aaccacataa tatcggcaat acttctttgc gaaagatgag ccgtaatcaa
     6601 cggaatagta aaggttgcaa tgctgcccgc actgtaatag atccccgttg cttttccttt
     6661 agcgtaggga aaacgttccg ccattaacgt caggccaatt tgtacaacac cgcctgcgga
     6721 ggtaaaaccg atgacgaaag caaatattat caccacataa aatgtgggat gcaggcagac
     6781 ggtgagcagg gcgataaatg agataaaggt gtacagcatc agtaatgttg ttgggcgaac
     6841 ggtattacga atgagtggag cggtaataaa tacacaaagc agcgaaccca cggtgtagat
     6901 actgagtaat ttgatcgaca tggtgtatga catgccggcg acaaattgtc cgtattgtgc
     6961 cagccactgg ctaaccagat aaaacgttgc cattgagata tagccatata gggaataact
     7021 ggctaaatcg ataattgaac agcgatgttc cgtagagctg gtggttttct ttatgacagg
     7081 taagtgacga cccggatggg gtgggaacgt acaacggtat aaaagcagag cgttaataaa
     7141 cataatgcct gcggcaatca taaaggacca accgaaccac agttcagccc acactaacag
     7201 gctgataatt agcggtaata aaaattgtcc gctggaaaca aatgctttaa ttaaaatatt
     7261 ggctgtaccc ggtgagcgtg gaaaagcttc catcaaactg ggataggtgc ctgcatcgag
     7321 aaaactgttt gccattcccg ccagaaagcc aaaaacataa gcgataatga tgttattggt
     7381 gtgcaggatg ccaaaaaaga aggccatata gcagcacatc ccgagcatga taaaagggcg
     7441 gcgaccaaag cgatcggata ataatcctgc aaaaagcaag acacttaatc gaccaatgcc
     7501 cagtgatgag ataactatcg agacgcccgc ggcattagtc tgccaaagcg tctccagcga
     7561 ggccatattc aggctcatca aaatgacgcc cataccatgc accaggtaat taaaatacaa
     7621 cccaagtgcg gtagggtaat agggattttt cataggttct acttagcaaa ggtgctttaa
     7681 aaagtgtaga gatgttgtgt cagtatccgt tcattattgt tgtaacaaag acaattcctt
     7741 tgtgtggaga aattaacaaa gccgctccag atcgtgtgca tataattcag cggctaccga
     7801 ttgatagatc ttccagtcaa taaaattgcc atcagggtag acttgttgaa acacggttaa
     7861 gtcggggaaa aaacgcatgg tgttgttgcc gatgcggtta tttattttct ctatgatggc
     7921 atgaagatgt ttttttcgtt gctgacgcat agccaacagt tgctcgacta cacgatcagg
     7981 aatggcgcat ttgccatttt cccactgacg ccagctttca ctgtcatcgg tctgggcaat
     8041 ccaggtcgcg cattcatcaa tagtcatggc aaaaatatgg cgaagtgcct ggagttcata
     8101 agcgttcatc gtgtaatggc tgcatgaaat aaacaataaa atataaataa cattatcagc
     8161 ctgctgacgg cttgaagaga gtcacagtat cttgtgcaac attattggaa agcgtgaggg
     8221 gcgagatagc aaagcggaaa aatgttaaaa aaaagcccat cgtgggagat gggcaaagac
     8281 tacacacagc aattcgttgt ttcactcagg ggatttccat gcttataaat caatatgttg
     8341 atttataacc gtgagctaat agtaggcata cggacttttt gcgtcgatca gattcgtctc
     8401 attagtttaa aaactgtaaa gaatttgcga caggtactac cagctgaatg gcggtaagca
     8461 ggaaaatatg gggaagagca agcaaatatc aattagcagg ggttaacaat caggcggcag
     8521 agccgcctga tgagaaatta cttattcgat ttctcgattt cgccaagttc ttcgagaatt
     8581 tcttccggtt ggtccgtcgg cggtgggact tcttccaccc acgcggcaaa aagacgccag
     8641 gaaacggcta acagaaccgg accaataaac aggccgatca tcccgaaagc aatcaaacca
     8701 ccaataacac cagagaggat caggatcagc ggtaaatcgg cacccatgcg aattaacatt
     8761 gggcggatga cgttatccag cgtgccaacc acaccgctcc acactaacaa taccgttccc
     8821 caggtggtat caccggtcca gtagagccag ataatcgccg gaatcagtac cggcaacggt
     8881 ccaagctgga caaggcagga gaggatcatt aacaccgtga gcaaagttgc ataaggtacg
     8941 ccggatacgg cgaggccgat accgccaagc actgcctgta ctaacgccgt caccaccaca
     9001 cccagcgcca ccgcgcggat agcctgtgcc gccagtagca cagcagcatc accgcgaacg
     9061 cctgccagac gggttgcaaa atggcgaatg ccttgtgcca cctgttcacc gcgccagtac
     9121 agcagggcac tgaagagaag catcagcgca caatgcacca taaagcgccc gatatgcgcc
     9181 gcctgcccaa cgaaccaggt ggtggtggtg ccaatataag gacggacttt cgccatgatc
     9241 gccgtacccc ccatatccag caagttgtgc cagcctgcat atagcttcgc gccaatcacc
     9301 ggaatggtat taagccacgc cagatcgggt aacgtcatgt caccgctgga aatggcttta
     9361 attagcggac cgctgccatc gacaatactg ttaaccaaca aggcaatagg gatgataaac
     9421 accatcacta ataacagcgt catcaccaga acggcgagag agcggcggcc aaacatgatc
     9481 ttttgcaaac gtaacaatac cggccaggtg gcgataacca ctgtaccggc ccatgcaaag
     9541 ccgagaataa agggttgaac aatccacaga catgccacaa tcatgatggc taaaaacagc
     9601 accgaaagca gaatttgtgc gacatccctg ggctgacgaa catttaccat aactactttt
     9661 cacctttttt gtgcgtcaaa tcgtcgacgc gacgtgaaca cgcgaaactc acctgcataa
     9721 tgatgagcaa tttcagcggt ttttaacagg tcgattctgc ccttaattct gatgggcgtg
     9781 taagaaaaaa atgtggtaca acaactcagg caacacgcaa acgatttact cgtcgtattt
     9841 caaattgcat gtgctgcggc tacgttcgtt caccccagtc acttactttt gtaagctcct
     9901 ggagttcact cgcttgccgc cttcctgcaa ctcgaaatcc atagagtaaa caaacatcac
     9961 ctttcagtgc gctattagca gcctgaaaga acagtgtatc gatctacatc acagaccgca
    10021 ggaaagggtc aatataatga ttccacagat ttcccaggca cccggcgtcg ttcaattggt
    10081 gcttaatttt ttgcaagagc tggagcaaca gggttttact ggcgatacgg cgacaagtta
    10141 tgccgatcgt ctgacaatgt cgaccgacaa cagtatttac caacttctcc ccgatgcggt
    10201 ggtatttccg cgttcaaccg cagatgtggc gctgatcgcc cgtcttgccg cgcaggaacg
    10261 ctattcatcg ctgatcttta ccccccgcgg cggcggcacc ggcactaacg gtcaggcgct
    10321 caaccagggg attattgttg atatgtcccg ccatatgaac cgcatcatcg aaattaaccc
    10381 tgaagagggc tgggtgcgcg ttgaggccgg ggtgataaaa gatcaactca atcagtacct
    10441 gaaaccgttc ggctactttt ttgcgccgga actttcgacc agcaaccggg caacgctcgg
    10501 tgggatgatc aataccgatg catccggtca gggatcgctg gtctatggca aaacgtcaga
    10561 tcacgtactt ggcgtacgcg cggtgttgtt ggggggcgat attctcgata cgcaaccttt
    10621 acccgtcgaa ctggcggaaa cgctgggtaa atccaatacc acaatcgggc gaatttataa
    10681 cacggtttat caacgttgcc gtcagcaacg ccagttaatc atcgacaact tccccaagct
    10741 taaccgcttt cttaccggtt acgatctgcg tcatgtcttt aacgatgaga tgaccgagtt
    10801 cgacctgacg cgcattctga cgggttcaga agggacgctg gcctttatta ccgaagcgcg
    10861 actggatatt acgcgcttgc ctaaagtgcg ccgtctggtg aacgtcaaat atgactcttt
    10921 tgactccgca ctgcgtaacg cgccgtttat ggttgaggcg cgggcgcttt cggtagagac
    10981 ggtggactca aaagtgctga atctggcgcg ggaagatatt gtctggcatt ccgtcagcga
    11041 gttgattacc gatgtgcctg acaaagaaat gctcgggctg aacattgtgg aatttgctgg
    11101 tgatgatgag acgctgattg atgagcgggt aaatgcactc tgtgcgcggt tggatgaact
    11161 gattgccagc caacaggcag gtgtgattgg ctggcaggtg tgccgcgagc tggcgggtgt
    11221 tgaacgtatc tatgcgatgc gcaaaaaagc cgttggtctg cttggcaatg ccaaaggtgc
    11281 cgctaagcca attccgtttg ctgaggatac ctgcgtaccg ccggaacatc tggccgatta
    11341 tattgctgaa tttcgcgcgc tgctcgacag ccacggctta agctacggta tgttcggtca
    11401 cgtcgacgca ggtgtcttgc acgtccgtcc tgcgctggat atgtgcgatc cgcaacaaga
    11461 gattttgatg aagcaaatct ctgatgacgt agtggcgctt accgcgaaat acggtggttt
    11521 gttgtggggc gaacacggca aaggttttcg tgccgaatac agccctgcgt ttttcggtga
    11581 ggaacttttt gcagaactgc gcaaagtgaa agcggcattt gacccgcata accgactcaa
    11641 cccagggaag atttgcccgc cagaaggtct cgatgcgccg atgatgaaag tggacgcggt
    11701 gaagcgcggt acattcgatc ggcagatccc cattgcggta cgccagcagt ggcgcggtgc
    11761 gatggagtgt aacggcaacg gtttatgctt caactttgat gcccgtagtt cgatgtgtcc
    11821 gtcgatgaag atcacccaga accggattca ttcaccgaaa gggcgcgcaa cgctggtgcg
    11881 tgaatggctg cgcttgttgg cggatcgcgg cgttgaccca ctcaaactgg aacaagaact
    11941 gcctgaatcc ggcgtcagtt tgcggacgtt aattgcccgc acgcgcaata gctggcatgc
    12001 gaataaaggc gaatacgact tctcacacga agtcaaagag gcgatgtcgg gctgtcttgc
    12061 ctgtaaagcg tgttcgaccc agtgccccat caaaattgat gtgccggagt ttcgctctcg
    12121 ttttctgcag ctctatcaca cccgttattt acgcccgctt cgtgaccacc tcgttgctac
    12181 ggtcgagagc tacgcaccgc tgatggcacg cgcgccgaaa acctttaact tcttcattaa
    12241 ccagccgctg gtgcgcaagc tctcggaaaa acatatcggc atggttgatt tgccactgct
    12301 gtcagtcccc tcgctacaac aacaaatggt ggggcatcgc tcggcgaata tgacgctgga
    12361 acagcttgaa gctctcaatg cagagcagaa agcgcgcacg gtgttggtgg tgcaggaccc
    12421 ctttaccagc tattacgatg cgcaagtggt ggcggatttt gtccgtctgg ttgaaaaatt
    12481 aggtttccag cctgtgttac tgccattttc gccaaatggc aaagcccagc atatcaaagg
    12541 ctttcttaat cgttttgcga agacggcgaa aaagacggcg gatttcctca accgtatggc
    12601 gaagctgggg atgccaatgg tgggcgtcga tccggcgctg gtactttgtt atcgcgatga
    12661 atataaactg gcgctgggcg aggaacgtgg cgagtttaac gtcttactgg cgaatgaatg
    12721 gctggcaagc gcacttgact cacagccagt ggctacagtc agcggtgaat catggtattt
    12781 ctttggtcac tgtaccgaag ttaccgcttt gccgggcgcg ccagcacaat gggttgcgat
    12841 atttgcccgt tttggcgcga aactggaaaa tgtcagcgtg gggtgctgcg gcatggcagg
    12901 gacttacgga catgaagcga aaaaccataa aaattcgctc gggatctatg agttatcctg
    12961 gcatcaggcg atgcagcgac tgccgcgtaa tcgctgtctg gcgaccggat attcctgccg
    13021 tagccaggta aaacgggtcg aaggcacggg ggtacgccat cctgtgcagg ctttactgga
    13081 gattattaaa tgatatggaa acgaaaaatc accctggaag cactgaatgc catgggtgaa
    13141 ggaaacatgg tggggttgct ggatattcgc tttgaacata ttggtgatga cacccttgaa
    13201 gcgacaatgc cagtagactc gcggacaaag cagcctttcg ggttgctgca tggaggggca
    13261 tccgtggtac tggccgaaag tatcggttcc gttgccggtt atttatgtac cgaaggtgag
    13321 caaaaggtgg ttggtctgga aatcaatgct aaccacgtcc gctcggcacg agaagggcgg
    13381 gtgcgcggcg tatgcaaacc gttgcatctc ggttcgcgtc accaggtctg gcagattgaa
    13441 atcttcgatg agaaagggcg tttgtgctgt tcgtcacgat taacgaccgc cattttgtga
    13501 cgttgtcacc agaaaagtgt gacgcatatt gcttttggtc attatctgca cagtaaacgg
    13561 tggtatactg tgcaggtctt gtctaaaagc gaggatgcta tgtctactca acttgatccc
    13621 acccagctgg ccattgaatt tttacgccgt gaccagagca atctttcacc tgcccaatat
    13681 ctcaaacgtc tgaaacaact agaacttgaa tttgctgacc tgcttactct ctcttctgct
    13741 gaattaaaag aagaaatcta ctttgcctgg cgtctgggcg tgcattgatc ctgacttaat
    13801 gccgcaattt tgccaaatcg acctggatta acctccgtcc aggttcgatg accttcttaa
    13861 tactgcttat ctatccgcct gatattgttc aaaaataata ctaatcgcag tttgtgttaa
    13921 aacggcgggt tagctttatg agattattct tatcgcccct tcaagagcta agccactgtg
    13981 agtgccggag ataagcgccg gatggggtag aaacccttaa gcctgtgtcg cacagactta
    14041 agggttttct tatttctgaa cgctgcactt ttttacctat cccttaaata gtcattttca
    14101 atacaacttt ttctttttca tcacagcctt aatgctcctt agataataat cattatctaa
    14161 caatgagata cctaattctt agcgtgcctg ttaacccaca catcagggtc tatgcttatt
    14221 aaaagcaggt aaaaagcatg ttttacgcta aagtccctgc gtttgctggg ttgaactgat
    14281 aatcattatc actaacatgc tgttatacgc tgaaagcgat gaagtgaggt aaatcgatgg
    14341 acatgcattc aggaaccttt aatccacagg atttcgcctg gcagggctta acgctgacac
    14401 ccgcagccgc tgtccacatc cgcgagctgg tggcaaagca gccgggtatg gtcggcgtgc
    14461 gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta tgtgctcgac agtgttagcg
    14521 agccggacaa agacgatctg ctgtttgaac acgacggcgc gaagctgttt gtcccgctgc
    14581 aagcgatgcc gtttattgat ggcacggaag tcgatttcgt tcgtgaagga cttaatcaga
    14641 tattcaaatt tcacaaccct aaagcccaga atgaatgtgg ctgtggcgaa agctttgggg
    14701 tataggcggt actatgtctc gtaatactga agcaactgac gatgtcaaaa cctggaccgg
    14761 cggcccgctg aattataaag aaggattctt cacccagtta gccaccgatg agctggcaaa
    14821 ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag cgtaatgagc cggagtggat
    14881 gctggagttt cgtctaaatg cctatcgcgc atggctggag atggaagaac cgcactggtt
    14941 gaaagcgcac tacgacaagc tgaattatca ggattacagc tactattcgg caccatcgtg
    15001 cggtaattgt gacgacactt gtgcgtctga acccggcgcg gtgcagcaaa ctggcgcgaa
    15061 cgccttttta agtaaagagg tggaggcggc gtttgagcag ttgggcgttc ccgtgcggga
    15121 aggcaaagag gtggcagtgg atgccatttt cgactctgtt tcggttgcca ctacttatcg
    15181 cgaaaaactg gcggagcagg gaattatttt ttgttccttt ggcgaggcga tccacgatca
    15241 cccggaactg gtgcgtaaat atctcggcac cgtggtgccg gggaatgaca acttctttgc
    15301 cgcacttaat gcggcggtag cctctgacgg tacgtttatt tatgtgccta aaggcgtgcg
    15361 ttgcccgatg gaactttcca cctattttcg cattaacgcg gaaaaaaccg ggcagtttga
    15421 gcgcaccatt ctggtggccg acgaagacag ctacgttagc tacattgaag gctgttccgc
    15481 tccggtgcgt gacagctatc agttacacgc ggcggtggtg gaagtcatca tccataaaaa
    15541 cgccgaggtg aaatattcca cggtacaaaa ctggttccct ggcgataaca acaccggcgg
    15601 tattctcaac ttcgtcacca agcgtgcgtt gtgcgaaggc gaaaacagca aaatgtcatg
    15661 gacgcaatca gaaaccgggt cagcgattac gtggaaatat cccagctgca ttttgcgcgg
    15721 cgataactcc attggtgagt tttactcagt ggcgctgacc agcggtcatc agcaagcgga
    15781 taccggcacc aagatgatcc acatcggtaa aaacaccaaa tcgaccatta tctcgaaagg
    15841 gatctctgcc ggacatagtc agaacagtta tcgcggctta gtgaaaatca tgccgacggc
    15901 aaccaatgcg cgcaatttca ctcagtgcga ttcaatgctg attggcgcta attgtggggc
    15961 gcataccttc ccgtatgtcg aatgtcgcaa taacagcgct cagctggagc acgaggcaac
    16021 gacatcacgt attggtgaag atcaactgtt ttactgcctg caacgcggga tcagcgaaga
    16081 agatgccatc tcgatgattg ttaacggttt ttgcaaagac gtgttctcgg aactgccgct
    16141 ggaatttgcc gttgaagcac aaaaactcct cgccatcagt cttgaacaca gcgtcggata
    16201 aggaataaac atgttaagta ttaaagattt acacgtcagc gtggaagata aagctatcct
    16261 gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac gccattatgg ggccaaacgg
    16321 ttctggcaaa agtaccttat cggcaacgct tgccgggcga gaagattatg aggtgacggg
    16381 cggcacggtt gagttcaaag gcaaagattt gcttgcgctg tcaccggaag atcgtgcagg
    16441 tgaaggcatc tttatggcct tccagtatcc ggtggaaatt cccggcgtca gtaaccagtt
    16501 tttcctgcaa acggcactta atgcggtgcg cagctatcgc ggccaggaaa cgctcgaccg
    16561 ctttgatttt caggatttga tggaagagaa aatcgctctc ctgaagatgc cggaagattt
    16621 attaacccgt tcggtaaacg ttggtttttc cggcggtgag aaaaagcgca acgatatttt
    16681 gcaaatggcg gtgctggaac cggagttatg cattcttgat gagtcagact ccgggctgga
    16741 tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg ctgcgtgacg gcaagcgttc
    16801 attcatcatt gttacgcact accaacgcat tctcgactac atcaagcctg attacgtcca
    16861 tgtgctgtat cagggacgaa ttgtgaaatc cggcgatttc acgttggtca aacaactgga
    16921 ggagcagggt tatggctggc ttaccgaaca gcagtaacgc actgcaacag tggcatcatt
    16981 tgtttgaagc tgaaggggcg aaacgctccc cgcaagcaca gcagcattta caacaattgc
    17041 tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa atatacgccg ctggacgggc
    17101 tgaccaatag ccagtttgtc agcattgcgg gggagatatc cccacagcag cgtgatgcct
    17161 tagcgttaac attagacgcc gtgcggctgg tgtttgtcga tggacgttac gtgcccgcac
    17221 tgagcgatgc aactgaaggc agcggatatg aagtgagcat taacgacgac cgtcaggggt
    17281 tacccgatgc tattcaggcg gaagtgtttc tgcatttgac ggaaagcctg gcacaaagcg
    17341 tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa gccattgctg ttaatgcata
    17401 tcacccaggg cgtggcaggt gaagaggtga atactgccca ttaccggcat catctggatc
    17461 tggcggaagg tgccgaagca acggtgatcg aacattttgt cagccttaat gatgctcgtc
    17521 acttcaccgg cgcacggttc actatcaacg tcgcagcgaa cgcccatttg cagcatatca
    17581 agctggcgtt tgaaaacccg ctcagtcacc actttgctca taacgatttg ttgctggctg
    17641 acgatgccac cgcatttagc cacagtttcc tgctgggtgg cgcagtatta cgacacaaca
    17701 ccagtacgca actcaatggc gaaaacagca cgctgcggat caatagcctg gcgatgccgg
    17761 tgaaaaacga ggtgtgtgat actcgtacct ggttggaaca caataaaggt ttttgtaaca
    17821 gccgacagtt gcacaaaact atcgtcagcg acaaaggccg cgcggtattt aacggtttga
    17881 tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat gaccaacaat aatctgctga
    17941 tgggcaaact ggcggaagtg gatacgaaac cgcagctgga aatctatgca gatgatgtga
    18001 aatgcagcca cggcgcgacg gtggggcgta ttgatgacga acagatgttt tatctgcgct
    18061 cgcgcgggat caatcagcaa gatgcccagc agatgatcat ttacgccttt gctgccgaac
    18121 tgacggaagc actgcgtgat gaggggctta aacagcaggt gctggcccga atcggtcaac
    18181 gactgccagg aggtgcaaga tgactttttc cgtcgacaaa gtgcgggccg actttccggt
    18241 gctttctcgt gaggtaaacg gtttgccgct ggcttatctc gacagcgccg ccagtgcgca
    18301 gaaaccgagc caggtgattg atgccgaggc tgagttttat cgtcatggct acgcggcggt
    18361 gcatcgcggt attcatacct taagcgccca ggcgaccgag aaaatggaga acgtgcgcaa
    18421 gcgggcatcg ctgtttatta atgcccgttc ggcggaagag ctggtgttcg tccgcggcac
    18481 gacggaaggg atcaatctgg tcgccaatag ctggggcaac agcaatgtgc gagcgggcga
    18541 taacatcatc atcagtcaga tggagcacca cgctaacatt gtcccctggc agatgctttg
    18601 tgcacgcgtt ggcgcagagc tgcgtgtgat cccgctcaac cccgacggta ctctgcaact
    18661 ggagacgctg cctacgctgt ttgatgagaa aactcgtctg ctggcaatta ctcatgtctc
    18721 caacgtgctt gggacagaaa atccgctggc ggagatgatc acacttgcgc atcaacatgg
    18781 cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat catccggtgg atgttcaggc
    18841 gctggattgc gatttttacg tgttctccgg gcataaactg tatggcccta ccggaattgg
    18901 cattctttat gtcaaagaag ccttgttgca ggagatgccg ccgtgggaag ggggcggttc
    18961 tatgatcgcc accgtcagcc tgagtgaagg cactacctgg accaaagcac catggcggtt
    19021 tgaagccggt acacccaata ccggaggcat cattggtctt ggcgcggcgc tggagtatgt
    19081 ttcggcgctg gggcttaata acatagccga gtatgaacag aatctgatgc attacgcgtt
    19141 atcacagttg gaatctgtac cggatctcac tctctatggc ccacaaaaca ggcttggcgt
    19201 tattgctttt aatctcggta aacaccacgc ctatgatgtt ggcagttttc tcgataatta
    19261 cggcattgct gtgcgtaccg gacatcactg cgcaatgcca ttaatggcct attacaacgt
    19321 ccctgcgatg tgtcgggcat cgctggccat gtataacacc catgaagaag tggatcgtct
    19381 ggtgaccggc ctgcaacgta ttcaccgtct gctgggataa cagggaggca ctatggcttt
    19441 attgccggat aaagaaaaat tgctgcgtaa ttttttacgc tgcgccaact gggaagagaa
    19501 atatctctac attattgagt tgggtcagcg tctgccagaa ttacgtgacg aagacaaaag
    19561 cccgcaaaat agcatccagg gctgccagag tcaggtgtgg attgtgatgc gtcaaaatgc
    19621 gcagggaatt attgaattac agggcgacag cgatgcggcc attgtaaaag ggcttattgc
    19681 ggtcgtcttt attctttacg atcaaatgac gccgcaggat attgtcaatt tcgatgtgcg
    19741 cccgtggttt gaaaaaatgg cgctcaccca acatctcacc ccatcacgct cacaaggtct
    19801 ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca cttagctaaa ctaaagggac
    19861 agctttcatc cggttggctc acattgccgg gtggtcaatg tttacactgg cctgcacagg
    19921 agtgttttgg tttcaggtga acataagcgc catccgacgt ttcagcgtga gtctccggca
    19981 aatacaggag gtttacaatg aaatgcgcgt ctttgcttac actcacgctt atcggcgctt
    20041 ttagcgccat ccaggctgcc tgggcggttg attatccgct accaccaacc ggcagtcgac
    20101 tggttgggca aaatcaaacg tatacggtgc aagaagggga taaaaacctt caggccatcg
    20161 cccgacgttt tgatactgcg gcaatgttga tccttgaagc caataacact atcgccccgg
    20221 tgccaaaacc cggtacgacg ataactattc cttcacaact attattacct gatgcgccgc
    20281 gtcaggggat tattgtaaac cttgcagagc tgcgccttta taattatccg ccgggagaaa
    20341 atatcgtgca ggtttatccg ataggtattg gtttgcaggg gctggaaacg ccggtgatgg
    20401 aaacgcgtgt ggggcagaaa atccctaacc caacctggac gcctacggcg ggcattcgtc
    20461 aacgttcgct ggaacgtggc attaaattac caccagtcgt tcctgccggg cccaataacc
    20521 cactaggacg ttacgcactg cgcctcgcgc atggtaatgg cgaatacctc attcacggca
    20581 ccagtgcgcc ggacagcgtc ggtttgcgcg tcagttcagg gtgtattcgc atgaatgcac
    20641 cggatattaa agccttattc tccagcgtgc ggacgggaac gccggtgaaa gtgatcaacg
    20701 aaccggtgaa atattccgtg gagcctaacg ggatgcgtta tgttgaagta catcgaccac
    20761 tatcggcaga agaacagcag aacgttcaga caatgccata cacactgcca gcaggcttta
    20821 cgcaatttaa agacaataag ggtgtagatc agaaattagt cgataaagcg ttgtatcgtc
    20881 gggcagggta tccggttgcg gtgagcagtg gagcaactcc cgcagccagc aatgcgcctt
    20941 cagtagagtc agcgcagaat ggtgaaccag agcaagggaa tatgttacgc gcgacgcagt
    21001 agcggtaaat ggcaggcaaa aaaaatggcg cacaatgtgc gccatttttc acttcacagg
    21061 tactattact tgcggtattt agtagccatg ttgtccagac gctggttagc acgagctgcg
    21121 tcatctttag cagcctgaac gtcggaacgc attgcgttca cgtcgttgct cagctggtca
    21181 actttagcgt tcagagtctg aacgtcagaa gacagctgat cgattttagc gttgctggag
    21241 caacctgcca gcagagtaga acccaggatt accgcgccca gtaccagttt agtagctttc
    21301 attattaata ccctctagat tgagttaatc tccatgtagc gttacaagta ttacacaaag
    21361 ttttttatgt tgagaatatt tttttgatgg gaaggcactt atttttgatc gttcgctcaa
    21421 agaagcatcg aacgctgggt tttagataaa aaaattgaga gaaaacagga tgcttccatc
    21481 ggattcatct tagataaagt gagattatat agtgaaatcc gatttgattt tttattgctt
    21541 ctggttatcg attaaataaa aaaagcgccc atcagggcgc ttcgatatac aaattaattc
    21601 acaaaagcaa tattacagga cgtgaacaga tgcggtgtta gtagtgccgc tcggtaccag
    21661 tgcaccagaa accataacta cgacgtcacc tttgtgtgcc agaccgctct gcagagccag
    21721 ttctttaccc agacggtaga aatcatcagt agaagtgatc tctttaacca gctgcggcac
    21781 aacgcctttg ctcagtacca actgatgagc cgttttttcg ttggtggtca gtgccaggat
    21841 ggtggcatcc gggaagtatt tacgtactgc gcgagcagat ttaccgccct gggtagcaac
    21901 cacgatcagc ggggcatcca gtttttcagc agtttcaacg gcaccacggc ataccgcttc
    21961 ggtaatgcgc agtttacggt tgtcattgtt gaactcgaga cggctgttca tcacgcggtc
    22021 ggtacgttcg cagatggtcg ccatgataga aaccgcttcc agcgggtatt taccttttgc
    22081 ggattcacca gacagcatca ctgcgtcagt accgtcgagg atggcgtttg caacgtcacc
    22141 ggcttctgcg cgagtcgggc gtgggttttt aatcatggaa tccagcatct gggtcgcagt
    22201 gataacgact ttacgtgcac ggatacattt ttcgatcatc atcttctggg cgaagataac
    22261 ttcttctacc gggatttcta cacccaggtc gccacgcgca accatgatgc cgtcagaggc
    22321 ttcgaggatt tcgtcgaagt tgttgaggcc ttcctggttt tcgattttgg agatgatgtg
    22381 gatgttttcg ccgccgtgcg ctttcaggtg ctcacggatt tcgataacgt cagaacgctt
    22441 acgaataaag gaagcagcaa caaagtctac gccttgttcg caaccaaaga tcaggtcctg
    22501 tttgtctttt tcagccagtg ctggcagggc aatggaaacg ccaggcaggt tcacaccttt
    22561 gttttcaccc aggtcgccgt tgttcagcac tttacagata actttgttac cttcaatggc
    22621 ggtaacttcc ataccgatga gaccatcgtc aaccagtacg gtgttgccaa cagacaggtc
    22681 agtagtgaaa ccttcatacg ttaccgcaac catttcgctg ttgccgataa cagatttatc
    22741 agtggtgaaa gtaaaggtct gacccgcttt cagagaaacg tcgttaccgc cttccagttt
    22801 catggtgcgg atttccggac ctttggtatc aagcaggata gcggcggttt taccagtttt
    22861 gctcatcacg ttgcgcagat tctgaatgcg ctgaccgtgt tctgcatagt caccatgaga
    22921 gaagttcaga cgcataacgt tcatgccagc gtccagcatt ttagctaaca tctcttcaga
    22981 ttcggttttc ggtccgatgg tgcaaacaat tttggtcttt ttcatgacag tcttagtctt
    23041 taagttgaga aggatgggag aaacttgctt tctgggcgca ctgccggaaa gcgaatccgg
    23101 tattacgaaa gtggtggtgc ctcgctctaa ggataggtga catcgaaaaa gcgtgcagag
    23161 gaatgtgtgc ttgtgtctca gcccaacgtg agtgcctgtt tttgagtcgt tgagttctac
    23221 agtccaaagt gctgaaacca ttcaagagtc aattggcgcg cattatacgc tgaaattaat
    23281 ccaaaaggaa aacaaaaaca gcgtttcaaa aaatatacaa cttgttagac aataaattgt
    23341 tatcaaagat ttaacgttcc agggaaaagg ttacgcaaaa acttcgctgt t
//
LOCUS       DAZKGF010000074        23169 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZKGF010000074 DAZKGF010000000
VERSION     DAZKGF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.074"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHOQV_002671"
     CDS             complement(<1..593)
                     /locus_tag="ACHOQV_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9631028.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHOQV_002672"
     CDS             complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHOQV_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP9631029.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEEEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHOQV_002673"
     CDS             complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHOQV_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP9631030.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHOQV_002674"
     CDS             complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHOQV_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP9631031.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHOQV_002675"
     CDS             complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHOQV_002675"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFP9631032.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLAGQISNDIILIDYTDFVRLTVKHSSQMAW"
     gene            complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHOQV_002676"
     CDS             complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHOQV_002676"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFP9631033.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHOQV_002677"
     CDS             complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHOQV_002677"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFP9631034.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRCWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4982..5704)
                     /gene="yheO"
                     /locus_tag="ACHOQV_002678"
     CDS             complement(4982..5704)
                     /gene="yheO"
                     /locus_tag="ACHOQV_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9631035.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="ACHOQV_002679"
     CDS             complement(5871..6683)
                     /gene="fkpA"
                     /locus_tag="ACHOQV_002679"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9631036.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6904..7122
                     /gene="slyX"
                     /locus_tag="ACHOQV_002680"
     CDS             6904..7122
                     /gene="slyX"
                     /locus_tag="ACHOQV_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFP9631037.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7171..7761)
                     /gene="slyD"
                     /locus_tag="ACHOQV_002681"
     CDS             complement(7171..7761)
                     /gene="slyD"
                     /locus_tag="ACHOQV_002681"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9631038.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7856..8056)
                     /gene="yheV"
                     /locus_tag="ACHOQV_002682"
     CDS             complement(7856..8056)
                     /gene="yheV"
                     /locus_tag="ACHOQV_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFP9631039.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8066..9871)
                     /gene="kefB"
                     /locus_tag="ACHOQV_002683"
     CDS             complement(8066..9871)
                     /gene="kefB"
                     /locus_tag="ACHOQV_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFP9631040.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVTVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9871..10422)
                     /gene="kefG"
                     /locus_tag="ACHOQV_002684"
     CDS             complement(9871..10422)
                     /gene="kefG"
                     /locus_tag="ACHOQV_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFP9631041.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10553..12466
                     /gene="yheS"
                     /locus_tag="ACHOQV_002685"
     CDS             10553..12466
                     /gene="yheS"
                     /locus_tag="ACHOQV_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9631042.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12466..13488
                     /gene="yheT"
                     /locus_tag="ACHOQV_002686"
     CDS             12466..13488
                     /gene="yheT"
                     /locus_tag="ACHOQV_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9631043.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNNLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13482..13700
                     /gene="yheU"
                     /locus_tag="ACHOQV_002687"
     CDS             13482..13700
                     /gene="yheU"
                     /locus_tag="ACHOQV_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFP9631044.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13754..14623
                     /gene="prkB"
                     /locus_tag="ACHOQV_002688"
     CDS             13754..14623
                     /gene="prkB"
                     /locus_tag="ACHOQV_002688"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFP9631045.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14678..15082)
                     /gene="yhfA"
                     /locus_tag="ACHOQV_002689"
     CDS             complement(14678..15082)
                     /gene="yhfA"
                     /locus_tag="ACHOQV_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFP9631046.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15384..16016
                     /gene="crp"
                     /locus_tag="ACHOQV_002690"
     CDS             15384..16016
                     /gene="crp"
                     /locus_tag="ACHOQV_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFP9631047.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16067..18157
                     /gene="yhfK"
                     /locus_tag="ACHOQV_002691"
     CDS             16067..18157
                     /gene="yhfK"
                     /locus_tag="ACHOQV_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFP9631048.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18224..19444)
                     /gene="argD"
                     /locus_tag="ACHOQV_002692"
                     /gene_synonym="dapC"
     CDS             complement(18224..19444)
                     /gene="argD"
                     /locus_tag="ACHOQV_002692"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFP9631049.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGA"
     gene            complement(19530..20093)
                     /gene="pabA"
                     /locus_tag="ACHOQV_002693"
     CDS             complement(19530..20093)
                     /gene="pabA"
                     /locus_tag="ACHOQV_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFP9631050.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20125..20727)
                     /gene="fic"
                     /locus_tag="ACHOQV_002694"
     CDS             complement(20125..20727)
                     /gene="fic"
                     /locus_tag="ACHOQV_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFP9631051.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTLHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20717..20884)
                     /gene="yhfG"
                     /locus_tag="ACHOQV_002695"
     CDS             complement(20717..20884)
                     /gene="yhfG"
                     /locus_tag="ACHOQV_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFP9631052.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20989..21561)
                     /gene="ppiA"
                     /locus_tag="ACHOQV_002696"
                     /gene_synonym="rotA"
     CDS             complement(20989..21561)
                     /gene="ppiA"
                     /locus_tag="ACHOQV_002696"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFP9631053.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21832..23013
                     /gene="tsgA"
                     /locus_tag="ACHOQV_002697"
     CDS             21832..23013
                     /gene="tsgA"
                     /locus_tag="ACHOQV_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFP9631054.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILIV
                     LAGLAAILMYVFNTGTPAYMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
BASE COUNT         5652 a         6305 c         5728 g         5484 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtca
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgacccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      481 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      541 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      601 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      661 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      721 cttcaggaat tccatggtgt atgatgcacg acctttggtc agagaacgca gctgagttgc
      781 atatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      841 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      901 ctcttccgga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      961 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
     1021 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
     1081 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
     1141 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
     1201 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
     1261 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
     1321 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacggtcaa cgatgatgtc
     1381 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1441 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1501 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1561 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1621 gatagcagca gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1681 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1741 accagagtta accacaccgg agtaaacacg gaagaaggtc aggttaccaa caaacgggtc
     1801 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgttcagc
     1861 cggagtgtct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1921 caggtaatcg attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1981 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttaatctc
     2041 ttcttcagtc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     2101 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     2161 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     2221 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     2281 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     2341 gcggtccatt ttgttaacga acgcaatgcg cggaacttta tatttgtttg cctgacgcca
     2401 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2461 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2521 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2581 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2641 gccgtcatga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2701 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2761 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2821 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2881 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2941 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     3001 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     3061 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     3121 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     3181 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     3241 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     3301 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3361 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3421 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3481 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3541 acccgggagg tctttaacac gaccgccacg gatcaggatc acggagtgct cctgcaggtt
     3601 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3661 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3721 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3781 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3841 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3901 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3961 gagtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tgagaatgat atcgttcgaa
     4021 atttgaccag ccaaaccgcg ggcaataagg tcttcgttca gggcatagac cttaatgggg
     4081 gcattacgca gactttcaag gtagcggtta ccgtcaactg cggcagttac accatcttgc
     4141 aataatagca gttcgtcgcc ctcacttagc aggcgcagca gcgcagcaaa atccgtcagc
     4201 cagggtgaac gatgtaatgt gtgcagcata aacgcctcaa aacctcaaaa taacatcgta
     4261 gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc gtggcttcga caacaaaggg
     4321 tgtctgcgga tctaacccgc gttcgcgcag tgaagccgca caaacccagc actgttcaat
     4381 gtcgtacaga cccaacaatt taaaagtggc gatgtaatca cgcgccagca ctgcatcggg
     4441 cttttgtcct ggcagcagct gaaaaacgcc atcagcaata aagaagacgg ccagatcgtc
     4501 agttaatgcg gaagttgcca gtaaagcatc taaaccttcc cgaccagcag ctgtaccatg
     4561 aggcacagta gaaaaaacaa acgcaattcg tttcatcaga actgtaccac cctgtcacag
     4621 gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg taaatcccgg ctgaaggttt
     4681 gacgaagcca gccccagtct tccggcctcc gtttcatcga cgataccgcg gcgtaacgct
     4741 gccgctacgc agatattcag cgccacacca tgttgcgcat tcaactgttg ccagcagcgc
     4801 acaaggtcaa attcatcgct tgccggtgag gtcaattggt tagcgttata gaccccttcc
     4861 cgataaaaaa agacgctgct taactcatgg ccttctgcaa tcagcgcctg cgcaaactga
     4921 aaagcactac tcgcctgttg cgtaccgtat gctggcccgg tcaccacgat ggcaaaacgc
     4981 attacttatc ttgcccctgg aaatcaccgc tcttgaactg gcggatgtag agatagacag
     5041 tgtgtttgga gatgttcagg cggtcagcaa cctggttgat cgcatcttta atatcgaaga
     5101 tccctttctc gtagagattc agcacgatct gacgattttt ggcgttatta gaaacattgc
     5161 gatcggcatt cacttcttcg atggtgaact ccagcgtttg ggtaaccaga tcttcaacag
     5221 aagaggcaaa gttgacgctt gaaccgacat ccggggtttc tggcggcaca aaggtgctca
     5281 taatctgcga gaagggaaca tcaagattca tattgatgca cagcaaacca attacacgct
     5341 gttcgcggtt acgaatcgcg atagtcaggg acttcattaa tacgccgctt ttggcgcgag
     5401 taaagtagca tttagaaacg ctgctatccg ctcccgtcat atcgtgcagc atacgtagcg
     5461 ccaggtcggt aattggcgaa ccaatcttcc ggcctgtatg ttcaccgtta gcaatgcgaa
     5521 tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat ttcacagtgg gagccaataa
     5581 gcatcgctaa cccgtccacc accgcttcgt aggatttcag aatatcaaaa tcggtctggt
     5641 cgaaaggacg ttgatccagt aaatccaact cactggtttc gttggttaaa agcgacctgg
     5701 acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa cttttcaggg caggctcatt
     5761 aatgatgcgg gtaactaaat taatacagcg gaggttccgc tttccagcac taattatatc
     5821 cggcctgtaa taaaaaaacc gccgcctggt caggcggcgg ttcttaatgc ttatttttta
     5881 gcagaatctg cggctttcgc atcagcttcc ggctttgcat cagccttcgg cgctggtttc
     5941 acatccagca gctctacgtc aaacaccagg gtagaattcg gtgggatccc cggaacaccc
     6001 gctttgccgt aagccagttc tggtggaata accagtttga tcttaccgcc tttcttgatg
     6061 ttcttcagac cttctgtcca acccggaata acaccgtcca gacggaaaga gagcggttca
     6121 ccacgggtgt aagagttgtc gaactcttta ccgtcgatca gcgtaccttt gtagttcacg
     6181 acaacagtat cgctgtcttt cggtgcttcg cctttaccgg cttcgactac ctgataaacc
     6241 agaccagttg aagaggtttt cacacctttc tctttggcaa atttctcgcg gtactcttta
     6301 cctttcgctt cgttatctgc cgcgtctttt tccatcttcg cctgagcaga agacttcacg
     6361 cgagcttcga atgcttgcag tgtctgttcg atctcttggt cggagagttt gctcttatca
     6421 gcaaatgcat cctgaacacc agcgatcagc tgatctttat ccagtttgat gcccagtttt
     6481 tcttgttctt taagagaatt ttccatgtaa cgacccagcg aagcacccag tgcataagct
     6541 gatttctgat cgtcattttt gaacgctgct ttgctgtcag cagttgtagc aggttttgca
     6601 gcttcagcag caaaagtgat cggtgcatgc agggcaacgg ccattgtggt cgccagcagc
     6661 gttactttaa acagtgattt catccatatc tccaggatcg gggcatctca ccccagggtt
     6721 aactattatc agaagggtac tataaagcgt tgtcgaacaa atctacatac agacacgccc
     6781 tattatcatc tattttcaga ctctttttgt ttaaattagt ttcgatgacc gcgaaatgag
     6841 tgctgtctcg ggcagcaaag ttaagtagaa tccgcggcga ccattcgaca aaagaggtga
     6901 atcatgcagg atttatcatt ggaagcacgc ctggcagagc tggagagccg actggctttt
     6961 caggaaatca ccattgaaga actgaacgtc acggtgaccg ctcatgaaat ggagatggcg
     7021 aaactgcgcg atcatctgcg tctgctgacc gagaagctaa aagccagcca gccgtcgaac
     7081 atcgcgtcgc aggctgaaga aacgccaccg ccacattatt gaggcgtaaa aaaagcgggg
     7141 attccccgct tttttgtcac tttttcggta ttagtggcaa ccgcaaccgc cgttaccttt
     7201 accgccgcag cagccttcgc caccgtgttc atgaccgtga tcgtggccat gaccaccgca
     7261 gcaaccgtcg tggtcgtgat cgtggtgatg atcgtgcgca ccgtgaacgt ggccatgagc
     7321 cagttcttct tcagtcgctt cgcgaatcgc cacaacttca acgttgaatt tcaggttctg
     7381 accggccagc atgtggttac catcaaccac gacgtgatcg tcttcaaccg cagtgatttc
     7441 aaccggtacc ggaccctggt cggtttcagc caggaaacgc ataccaacct gcagttcatc
     7501 aacgcccata aatacgtctt taggaacgcg ttgcaccagg ttttcgtcgt actgaccgta
     7561 agcgtcgttc gcgccaacag cgacatcaaa tttgtcgcca acttcgtgac cttccagcgc
     7621 cgtttccagg ccagagatca gggaaccgtg accatgcagg tagtccagcg gcgcactcac
     7681 cggagactca tcaaccaaca caccgtcttc tgtacgtacc tgataggcca ggctgaccac
     7741 caggtctttt gctactttca tgatatctcc tgagcatggg aagaatagtg gcgcagattg
     7801 tagcggaatt ctgcagccgt gtactcacta gcttaaaaaa actcggcgca tatcgctagt
     7861 ccggatgaaa aatcccgatc acttgctcat ctttgcgaac gtgatcgcgg gcttctttgt
     7921 ccgcttctcg catctgatgt ccgcacttaa cacattcaac aatatcaata ttattttcgc
     7981 gccacatcgc cattgaatcc tgcgcctgac aggccgggca ttttgccccc gcaataaaac
     8041 gttttcggat tgccatcttt accctctact caaattcatc ccagccgtcc agctggcgtc
     8101 gttcttgttg catttcacgc tggaaaatct cttccagttc gcgtcgggct tccctggcgc
     8161 gagaaatttg tacggtgtcg gcatgcattg ggatgagctc ccgcaacatc cgcatatcca
     8221 ggcggcgaaa atgcagttgc gcccgctgcg cctgatgtgg atgcatgcct agcgtgacca
     8281 gcgtcttgcg ccccagctct aacgcactgg agaatgtctc acgagaaaac tgcgtcaccc
     8341 ctgcctgtaa taactcatgc gcctccacac gtccgcgcgc tcgcgcaaga atatgtaaat
     8401 gcggaaagtg ctgctggcat atttccacca gtttcatggt gtcttccggc tcattacagg
     8461 taatgacgat agactcagcg gcctctgcac ccgcagaacg taaaagatct acctgcgtgg
     8521 cgtcgccgta ataaactttg tagccatatt tgcgcatcag gttaacggcg ctgatatccc
     8581 gctccagcac agtaatgcgc attttatttg ccatcagcaa acgaccaatc acctgaccaa
     8641 aacgcccgaa gcccacgaca atgacctgcg gtttatcatc gttgacccac ggtttttcgt
     8701 cttcttcttc cggtccgtta aactggcggg atagccattt atccaccagc ttcatcagca
     8761 acggcgtggt catcatggaa agcgtcaccg tcaccagcaa cagcgccatc tggtcgccct
     8821 gaaacaaccg ttgcgaagaa gcggtagaaa agagcacaaa ggcaaactca ccgccctgac
     8881 tcaacacgcc agcaaactgc atccgctctg agctacgcac gccatataat cgcgccagca
     8941 gatacagcac gagaattttc accgtcacca gcacaaccac gcttatcact acccacaaca
     9001 gatgggtata aagcaccccg agattgagcg acatgccgac agagataaag aacaaaccga
     9061 gcagcaagcc tttgaaggga tcgatagctg tttccagttc gtggcgatat tcgctttctg
     9121 ccagcaacac gcccgcaata aacgtaccga gcgccatcga cagccccagc gcatccataa
     9181 acaatgcgga acccaacacc agcagcagcg tcgcggcggt gaacacttcc cgcacgccag
     9241 aagctgcaat aaaacggaat accggacgca gtaaatagcg cccgccaatc agcatgccga
     9301 caaacgccag caccttcatg ccgatcttca tccagtcgaa atgttcgtct gccgaacccg
     9361 ccaacaacgg cactaacgcc agcgccggga tcactgccag atcctgaaac agcagaaccg
     9421 aaaaacctag ctgcccggat tcgctgcgat tcatcccttt ctcacgcatc agttgcaacg
     9481 ccattgcggt tgaggacatc gcaaggccaa taccaccgac caccgccgcc tgccaggcga
     9541 aatccgtcag catcaataat cccgccagca acgccgcgct taacagcacc tgtgccgcac
     9601 ctacgccaaa aatcgaacgc cgcaattgcc aaagcttgga gggattcaac tccaggccga
     9661 tgataaacat caggaacacc acgccgagtt ccgaaaagtg gaggatctca tcgacgtcgc
     9721 taataaaacc cagcccccac gggccaattg caatccctgc cagcaaatat cccaacacag
     9781 cgccaatacc cagccgcgat gccagcggca ccgcagccac cgccgcgaag agaaacaaca
     9841 ctcctgcgag taaaaaatcg gaaccttcca tcagcggcct cctggagaca gcggatttgc
     9901 cagccagtca ccgtaggctc tggcgtgact cgccagctcc tgtgcgcttt gccgtctcgc
     9961 ccagtaaata atgatgggac tcaaccaatg catccggcac atgcccgccg ctagttcaaa
    10021 ggggcgcaga acatcgctca tcgggtagcg attcaaggcg tcataacggt aagcactttc
    10081 cggctcgccg gtggtaatca cgctacgcca gtactttccc gccagttggt ttcctcccgg
    10141 cccgctggca aaaccacggc ttaatacccg gtccagccac tctttcagta gcgccgggca
    10201 gctataggta taaagaggat gctgaaagac aatcacctcg tgctcgcgca gtaatgcctg
    10261 ctcacggggg atatcaataa aaaaatcggg atagtgcgcg taaaggtcgt gcacggtaac
    10321 attgctgagc tgcgtggccg gtttaagcag tacccggttt gccaccgagt cctgagattc
    10381 cggatgggca tacagcagca aaactttcgc tggctgagac atcatccccc tcccggtatg
    10441 gtttttgtgt atagtcgctg ttttgggcta ccattgcgcc cggtgcggca gctcgcccat
    10501 acattacatt atcataatga taagttaaca tagtctgaac atacggcgcc ttatgattgt
    10561 tttttcctcg ttacaaattc gtcgcggcgt gcgcgtcctg ctggataatg ccaccgccac
    10621 catcaacccc gggcagaaag tcggcctggt gggtaaaaac ggctgtggta aatcgaccct
    10681 gctggcattg ctgaaaaatg aaatcagcgc cgacggcggc agctacacct ttccgggaag
    10741 ctggcaactg gcgtgggtga atcaggaaac gccggcgtta ccgcaagcgg cgctggaata
    10801 tgtcattgac ggcgaccgtg aatatcgtca actggaagcg cagctacacg acgccaacga
    10861 acgtaacgac gggcacgcca tcgccactat tcatggcaag ctggatgcta tcgacgcatg
    10921 gagtattcgc tcccgtgccg ccagcctgct gcacggcctc ggtttcagca atgaacaact
    10981 ggagcgcccg gtaagtgatt tctccggtgg ctggcgtatg cgtcttaacc ttgcccaggc
    11041 gctgatttgc cgttcagact tgctgctgct cgatgaaccg actaaccacc tcgatctcga
    11101 tgccgttatc tggctggaaa aatggctgaa gagctatcag ggcacgctga tcctgatctc
    11161 tcacgaccgc gacttcctcg atccgatcgt tgataaaatt attcatatcg aacaacaaag
    11221 catgttcgag tacaccggca actacagttc gtttgaagta cagcgcgcca cccgtctggc
    11281 gcagcaacaa gcgatgtatg aaagccagca ggaacgcgtg gcgcatctgc aaagttatat
    11341 cgaccgtttc cgtgccaaag ccaccaaagc gaagcaggcc cagagccgta ttaagatgct
    11401 ggagcgtatg gagctgattg ccccggcgca cgttgacaac ccgtttcgtt ttagcttccg
    11461 cgcgccggaa agcctgccta atccgttatt aaagatggaa aaagtcagcg caggctatgg
    11521 tgatcgcatt attctcgact cgattaaact gaatctggtc cccggctcgc gcattggtct
    11581 gctaggccgc aacggcgcgg gtaaatcgac attaatcaaa ctgttagccg gcgagcttgc
    11641 gccagtcagc ggtgaaattg gtctggcgaa agggatcaag ctcggctact tcgcccagca
    11701 tcaactggaa tacctgcgcg ccgacgaatc gcctattcaa catctggcac gtttagcgcc
    11761 gcaggaactg gagcaaaaac tgcgtgacta cctcggcggc tttggtttcc agggcgataa
    11821 agtgacggaa gaaacgcgcc gcttctccgg tggggaaaaa gcccgcctgg tgctggcatt
    11881 aattgtctgg cagcgtccga atctgctgct gctcgacgaa ccgactaacc accttgacct
    11941 cgacatgcgt caggcactga ctgaagcatt aatcgagttc gaaggcgcgc tggttgtcgt
    12001 ttcgcacgac cgtcatttgc tgcgttccac cactgacgat ctctacctgg ttcacgatcg
    12061 taaagtcgaa ccgttcgacg gcgatctgga agattatcaa cagtggttga gcgacgtaca
    12121 aaagcaggaa aaccagaccg acgaagcgcc aaaagagaac gcaaacagcg cccaggcacg
    12181 taaagatcag aagcgtcggg aagcggagct gcgtgcgcaa acccaaccgc tgcgtaaaga
    12241 gatcgcccgt ctggaaaaag agatggaaaa gctgaacgca cagctggcgc aggcggaaga
    12301 gaaactcggc gacagcgaac tgtacgatca aagccgtaaa gcggagttga ccgcctgcct
    12361 gcaacagcaa gccagcgcca aatccggcct ggaagagtgc gaaatggcgt ggctggaagc
    12421 ccaggagcag cttgagcaga tgctgctgga aggccaaagc aactgatggc gcagataacg
    12481 acgaccgatg ccaatgaatt cagcagcagt gctgaattca cccctatgcg cggctttagc
    12541 aattgtcatc tacaaaccat gctgccgcgt ctgtttcgtc gcaaggtgaa attcaccccg
    12601 cactggcagc gactggagtt gcccgacggc gattttgtcg atctcgcgtg gagtgaagat
    12661 cctgcacagg cgcggcataa accgcgttta gtcgtgtttc acgggctcga aggcagtctc
    12721 aatagccctt acgcccacgg tctggttgag gcggcgcaaa agcgcggctg gctgggcgtg
    12781 gtgatgcatt ttcgcggatg cagcggtgaa ccaaaccgta tgcaccgcat ttaccattcg
    12841 ggtgaaaccg aagacgccag ctggttttta cgctggctgc agcgcgaatt tggacatgcg
    12901 ccaacggctg ccgtcggcta ttcgctcggc ggtaatatgc tcgcctgttt gctggcaaaa
    12961 gaaggtaata acctcccgat tgatgcagcg gtaattgtct ccgcgccatt tatgctggaa
    13021 gcctgtagtt atcatatgga aaagggcttt tcccgcgttt atcagcgtta cttgctgaac
    13081 ctgttaaaag ccaatgccgc gcgcaagctg gcagcctacc ccggaacact gccgattaat
    13141 ctcgcgcagt taaaatcggt acgtcgcatc cgtgaatttg acgatctaat caccgccaga
    13201 attcacggct acgctgacgc tatcgactat tatcgtcagt gtagcgccat gccgatgctc
    13261 aatcggatcg ccaaaccgac gctgattatt cacgccaaag acgatccgtt tatggatcat
    13321 caggtgatcc cgaaaccgga aagtctcccc ccgcaggtgg agtatcaact gactgaacat
    13381 ggcggtcatg ttggctttat tggcggtaca ttacttcatc cgcaaatgtg gctggagtca
    13441 cgcattcctg actggttaac aacgtatctg gaggcgaaat catgttgatt ccgtggcaag
    13501 acctctcccc cgaaacgctg gaaaatttaa ttgaaagctt tgtgttacgt gaaggcaccg
    13561 attatggtga acatgagcgt acacttgaac agaaagtcgc cgacgtcaaa cgccagctac
    13621 agtgcggaga agcggtgctg gtatggtcgg agctgcacga aacggtcaat atcatgccgc
    13681 gcagtcagtt tcgcgaataa cctgcaaccg tcggcggacg agataataat gatagctaag
    13741 tcatggagtt actatgtctg ccaaacatcc ggtcattgcg gtaacaggat ccagcggcgc
    13801 ggggaccacc accaccagcc tcgcgtttcg taaaatattc gcgcagttaa atctgcatgc
    13861 agccgaggtg gaaggcgaca gttttcaccg ttacacccgc ccggaaatgg acatggcgat
    13921 ccgcaaagca cgcgacgccg ggcggcatat cagctacttc ggccccgaag cgaacgactt
    13981 cggcctgctg gaacaaacct tcattgaata tggtcagagc ggcaaaggga aatctcgcaa
    14041 atatctgcat acctacgatg aagccgtacc gtggaatcag gtaccgggga cattcacccc
    14101 ctggcaacct ttaccggaac ccactgatgt actgttttat gaaggtttac acggcggcgt
    14161 agtcacgcca cagcataacg ttgcgcagca tgtggactta ctggtcggcg tggtgcctat
    14221 cgttaacctt gagtggattc aaaaactgat ccgcgacacc agcgagcgcg ggcactcacg
    14281 agaagcagtg atggactcgg tagtgcgttc aatggaagac tatatcaact acatcacacc
    14341 gcagttttcc cgcacccatc ttaacttcca gcgcgttccc accgtcgaca cttcaaaccc
    14401 gttcgcggca aaaggtatcc cgtcgctgga tgaaagcttt gtggtgatcc attttcgtaa
    14461 tctggaaggg atcgatttcc cctggctgct ggcgatgttg caaggctcat tcatttccca
    14521 catcaatacg ttagtggtac cgggcggcaa aatgggtctg gcaatggaat taattatgct
    14581 gccgctggtg caacgattga tggaaggaaa gaaaatcgag taactttgct attacgccgg
    14641 ataaaatact atccggcttc acaatgggat agttaaatca cgcggcaacc acttcatacg
    14701 agtgagtaat attcaccgct ttttccagca tcaacgccac tgaacaatat ttctcggcag
    14761 agagatcaac cgcacgtgca accgctgcgt ctttcaggtc gcgaccggta acgataaaat
    14821 gcagattaat gtgcgtaaac aggcgtggtg cctcttcgcg gcgttcagag gtcaatttta
    14881 cttcacaatc gaccacatcc tgacgccctt tttgcaggat agaaaccaca tcgatggcac
    14941 tgcaaccccc cgccgccatc aacaccattt ccatcggact tggtgcttta tcgcctgagt
    15001 tgccgtccat taaaatctgg tgaccagagg cggattcgcc cagaaaagtt aacccttcga
    15061 cccacttcac tcgcgcttgc atttttgcta ctccactgcg tcaattttcc tgacagagta
    15121 cgcgtactaa ccaaatcgcg caacggaagg cgacctgggt catgctgaag cgagacacca
    15181 ggagacacaa agcgaaagct atgctaaaac aatcaggatg ctacagtaat acattgatgt
    15241 actgcatgta tgcaaaggac gtcacattac cgtgcagtac agttgatagc cccttcccag
    15301 gtagcgggaa gcatatttcg gcaatccaga gacagcggcg ttatctggct ctggagaaag
    15361 cttataacag aggataaccg cgcatggtgc ttggcaaacc gcaaacagac ccgactctcg
    15421 aatggttctt gtctcattgc cacattcata agtacccatc caagagcacg cttattcacc
    15481 agggtgaaaa agcggaaacg ctgtactaca tcgttaaagg ctctgtggca gtgctgatca
    15541 aagacgaaga gggtaaagaa atgatcctct cctatctgaa tcagggtgat tttattggcg
    15601 aactgggcct gtttgaagag ggccaggaac gtagcgcatg ggtacgtgcg aaaaccgcct
    15661 gtgaagtggc tgaaatttcg tacaaaaaat ttcgccaatt gattcaggta aacccggaca
    15721 ttctgatgcg tctgtctgca cagatggcgc gtcgcctgca agtcacttca gagaaagtgg
    15781 gcaacctggc gttcctcgac gtgacgggcc gcattgcaca gactctgctg aacctggcaa
    15841 aacaaccgga tgctatgact cacccggacg gtatgcaaat caaaattacc cgtcaggaaa
    15901 tcggtcagat tgtcggctgt tctcgtgaaa ccgtgggacg cattctgaag atgctggaag
    15961 atcagaatct gatctccgca cacggtaaaa ccatcgtcgt ttacggcact cgttaatccc
    16021 gtcggagtgg cgcgttacct ggtagcgcgc cattttgttt cccccgatgt ggcgcagact
    16081 gatttatcac cccgatatta actatgcact gcgacaaacg ctggtgctat gtttgcccgt
    16141 ggccgttggg ttaatgcttg gcgaattacg attcggcctg ctcttctccc tcgttcctgc
    16201 ctgttgcaat attgcgggcc ttgatacgcc tcataaacgt tttttcaaac gcttaatcat
    16261 tggtgcgtcg ctgtttgcca cctgtagctt gctgacacag gtgctactgg caaaagatgt
    16321 tcccctgccc tttttgctga ccggattaac gctggtactt ggcgtcactg ctgaactggg
    16381 gccattgcac gcaaaattgc ttcccgcatc gctgctcgcc gccattttta ccctcagtct
    16441 ggcaggatac atgccggtct gggaaccgtt gctcatctat gcgttgggca ctctctggta
    16501 cggattgttt aactggtttt ggttctggat ctggcgcgaa caaccgctgc gcgagtcact
    16561 aagtctgctg taccgcgaac tggcagatta ttgtgaagcc aaatacagcc tgcttaccca
    16621 gcacaccgac cctgaaaaag cgctgccgcc gctgcttgtg cgccagcaaa aagcggtcga
    16681 tctaattacc cagtgctatc agcaaatgca tatgctttcc gcgcaaaata ataccgatta
    16741 caagcggatg ctgcgtattt tccaggaggc gctggatttg caggaacata tttcggtcag
    16801 tttgcatcag ccggaagagg tgcaaaaact ggtcgagcgt agccatgcgg aagaagttat
    16861 ccgctggaat gcgcaaaccg tcgccgctcg cctgcgcgtg ctggccgatg acattcttta
    16921 tcatcgcctg ccgacgcgtt ttacgatgga aaagcaaatt ggcgcactgg aaaaaatcgc
    16981 ccgccagcat ccggataatc cggttgggca attctgctac tggcatttca gccgcatcgc
    17041 ccgcgtgctg cgcacccaaa aaccgctcta tgcgcgtgac ttactggccg ataaacagcg
    17101 gcgaatgccg ttacttccgg cgctgaaaag ttatctgtca ctaaagtctc cggcgctacg
    17161 caatgccgga cgactcagtg tgatgttaag cgttgccagc ctgatgggca ccgcactgca
    17221 tctgccgaag tcgtactgga tcctgatgac ggtattgctg gtgacgcaaa atggctatgg
    17281 cgcaacccgt ctgaggattg tgaatcgctc cgtgggaacc gtggtcgggt taatcattgc
    17341 gggcgtggcg ctgcacttta aaattcccga aggttacacc ctgacgttga tgctgattac
    17401 caccctcgcc agctacctga tattgcgcaa aaactacggc tgggcgacgg tcggttttac
    17461 tattaccgca gtgtataccc tgcaactgtt gtggctgaac ggtgagcagt acatccttcc
    17521 gcgtcttatc gataccatta ttggttgttt aattgctttc ggcggtactg tctggctgtg
    17581 gccgcagtgg cagagcgggt tattgcgtaa gaacgcccat gacgctttag aagcctatca
    17641 ggacgcgatt cgcttgattc tgagcgagga cccgcaacct acgccactgg cctggcagcg
    17701 aatgcgggtg aatcaggcgc ataacactct gtataactca ttgaatcagg cgatgcagga
    17761 accggcgttt aacagccatt atctggcaga tatgaaactg tgggtaacgc acagccagtt
    17821 tattgttgag catattaatg ccatgaccac gctggcgcgg gaacaccggg cattgccacc
    17881 ggaactggca caagagtatt tacagtcttg tgaaatcgcc attcagcgtt gtcagcagcg
    17941 actggagtat gacgaaccgg ggagttctgg cgatgccaat atcatggatg cgccggagat
    18001 gcagccgcac gaaggcgcgg caggtacgct ggagcagcat ttacaacggg ttattggtca
    18061 tctgaacacc atgcacacca tttcgtcgat ggcatggcgg cagcgaccgc atcacgggat
    18121 ttggctgagt cgcaagttgc gggattcgaa ggcgtaatac aggctaaacc atcggatgag
    18181 gcgttcacgc cgcatccgac atcttttgcc tgacgcgtct tatttacgcc ccaatcacct
    18241 tcgccaccgc gtgggcgaaa cgatgcatcc cttcatcgat atccgcatct tccaccacca
    18301 gcgacggcgc aaaacgcatc acatcaggcc cggcattcag caccattacg ccttcctctg
    18361 caccggcata caggaaatca cgcgcccgac ctttgtactg tggtttcagc tccgcgccaa
    18421 ttaacagccc cataccgcga atatcgctaa acacatcgta ctgctgatcg atcttctgca
    18481 aatggtcaac aaaacgctgg cgtttcgcct gaatgccttc cagcacttca ggggtattga
    18541 tgatatcaaa cgccgcgccc gctactgcac aggccagagg attaccgccg taggtggaac
    18601 cgtgagaacc aggatgaaat gcagaagcaa tttccgccgt ggtcagcatg gcgctaatcg
    18661 ggaagccgcc gcctaacgct ttcgcagagg tcagaatatc cggcgtcaca ccgtagtgca
    18721 tgtaagcaaa caaatcgcca gtccgcccca tcccgcactg cacttcatca aacaccaata
    18781 atgcctgatg ttgatcgcac agctcgcgca agccctgcaa aaactctggc gtcgctgccg
    18841 tcacaccgcc ctcgccctgg atcggctcaa ccaccaccgc acaggtatgg tcatccatca
    18901 ccgctttcac tgcgtggaga tcgttaaaag gcacgtggat gatgtctgcc ggttttggcc
    18961 caaagccgtc ggaatatttt ggctgcccac ccaccgaaac ggtaaacagc gagcgaccat
    19021 gaaaagcgtt atggaaggca ataattttgg ttttgaacgg gctatgacgc acacaggcgt
    19081 aatggcgtgc cagtttaaag gcggtttcgt tagcttccgt gccggagttc ataaacacca
    19141 cgcgttcggc aaaagttgcc tcaatcagtt tacggccaag acgcagcgcc ggctcattgg
    19201 tgaaaacgtt actaatatgc cacagagttt cgccctgggt ttttaacgcg ttcaccagcg
    19261 caggatggca atggcccaac gccgtaactg caatgccacc cgcgaaatcg acatactcct
    19321 tgccctgttg atcccagatt cggctgccct gaccttttac cggaataaac tctgccggag
    19381 cataaatcgg cagaatcact tcatcgaaag tcgcgcgtgt aattgctgtt tgttcaattg
    19441 ccatctcatg atcaccctgt tacgcataaa taaatatgaa attataatca caaaatatgc
    19501 ataaaaaatc actgaatggc aatcagaaat cagcgatgca gaaaattagc cagcagttga
    19561 tgtccttgtt cgctgagaat actttctgga tggaattgca caccttccag atcccactgg
    19621 cgatggcgaa tccccataat ctcgcgggtt tcgctccagg ccgtcacttc aaagcactcc
    19681 ggtaatgagt caggttccac caccagcgaa tggtagcgtg tcacggtgag tggatttgcc
    19741 agccccttaa atacgcccac accattatgt gtaatcggcg aggttttgcc gtgcatgacc
    19801 tttgcggcgc gcacaacttt gccgccaaat gcctgcgcca ttgcctgatg accgaggcag
    19861 acgccaagaa tcggcaagcg tccggcatag tgacgaataa cgtcaaggga gatcccggct
    19921 tcatctggcg tacaggggcc gggtgagatg acaatttttt gtggtttaag ggcgtcgata
    19981 tccgccaacg tcaacgcatc gttgcgctta accaacacat ccgcccccag ttcacaaaag
    20041 tactggtaga ggttccaggt aaaagaatcg tagttatcaa taagcaggat catggcggct
    20101 ccggtacgaa agaaccgcgc tattttactc agattccccg gcttcgctta ccactttgct
    20161 aaatatcatc tgcagcgcgg tgagatcccc cattgcccca ttctgatttg cctgattcca
    20221 ggcctctttt tcaataccct gccagctcag ttgatatccg gcatgaatcg ccagttgctc
    20281 gaagaagatc cgctgtgcca aaccacttcc cacccggaag ggatgcagca cgttgatttc
    20341 acaatagtaa tgcgccagcc gctcgacgaa cttcgctttc tccaggccaa ccagataacc
    20401 ttcttcctcc agatcctgca tcagggcatt gccctctttt tcgatataag caaagtggca
    20461 gaacggcgta tcgccctgat aaatatcgac ttcgcgcagt tgccccgccc agtcgaaaat
    20521 atcctgatac aactggcgat gaagagtacg caaatgcggt aaaccccgaa ccaacgggcc
    20581 aagttcaatg gtcgcagcac gcagcgccgt catttcgtaa gcggcctgtt ccagccgctg
    20641 ctgctggcgg atgttcagcc ggttacgcat gatatcaagg cctggataaa gatacggatc
    20701 gcgcccttcg ccgaatttat cgctcatagt gcctcctcag cgcttcaaga cgcgctaaag
    20761 cctctgcggc agtaagagtg actaaaggca tctcgacgcc ttcaagacgg cgactggcct
    20821 ggaaattacg attacgctga agctcccaga gacgggactt ttgcttatcg gtgagtttct
    20881 tcacttgatg cctccctgaa cgtgtccatt tgccacaagt ataagcggca aatccgggtt
    20941 acgccgggag agcagaagcg cgggcaagat tgcccgcgcg agaaatcatt acggcaggac
    21001 tttagcggaa aggataacta ccggttttga cggcacattc tggtacggac caacgtcatg
    21061 agtcggcacc tgggaaatct tatcggcaac gtccatgcct ttcaccactt taccaaatac
    21121 cgcgtaaccg aaatcacgct gaccgtggtc aaggaaggcg ttatcggcaa cgttgataaa
    21181 gaactggctg gtggcgctgt ctttgtcagc ggtacgcgcc atcgcgatgg tgccacgcgt
    21241 gttgcgcagg ccgttatcag cttcattttt gattggcggg tttggttttt tctgctgcat
    21301 ctgctcggtg aaaccgccgc cctgaatcat aaagccagga atgacgcggt gaaaggtcgt
    21361 gttgttataa aaaccactgt tcacataatc gacaaagttt tgcacagaca ctggcgcttt
    21421 ttgtttatcc agctccagtt cgatgttacc agctgaggtt gtcaacaata cgtgcgggtc
    21481 ccctttcgct gccattgctg cgggagaaag agcagaaaga gcgaaaacag cagccatcgc
    21541 cgccagggtc gatttgaaca tgagatttcc ttaacaaaca gagcagaaaa aaagcaagtg
    21601 aagagattct aaatagcctt caagaacaag gccatccctt tacctaattt tacgtatctg
    21661 aaatatatgt aacctgagga taacttttta ttccgtgatc aaaatcacct cttaaaatgc
    21721 aatttagcaa ccgattgcaa taaaacattt aatcagatca caaaatcgcc taaaatcgcc
    21781 cgctcgcggc atagcccgct atgccatttt atttttgaac gcaggccaat catgactaac
    21841 agcaatcgca tcaagctcac atggattagc tttctttcct acgcactgac cggtgcgttg
    21901 gttattgtca ccgggatggt gatgggaaat atcgccgatt atttcaatct gcctgtttcc
    21961 agcatgagta acaccttcac cttcctcaat gccggcattt taatctctat cttcctcaac
    22021 gcctggctga tggaaatcgt cccgttgaaa acgcagttac gttttggctt tctcctgatg
    22081 gtgctggcgg ttgccggttt gatgttcagc cacagcctgg cgctgttctc ggcggcgatg
    22141 ttcattctcg gggtggtcag cggcatcacc atgtcgattg gtacattcct ggtaacacaa
    22201 atgtatgaag gacgtcagcg cggttcacgc ctgttattta ccgactcctt cttcagtatg
    22261 gccgggatga ttttcccaat gatcgccgcg tttctgctgg cacgtagcat tgagtggtac
    22321 tgggtttatg cctgcatcgg gctggtgtat gttgctattt ttattctgac cttcggctgt
    22381 gaattcccgg cactgggcaa acatgcgcca aaaacagatg ctccggtaga gaaagaaaag
    22441 tgggggatcg gcgtactgtt tctctctgtt gcggcgctgt gctacatcct cggtcagtta
    22501 ggttttatct cctgggtgcc ggaatatgcc aaaggcctgg gcatgagcct gaacgacgca
    22561 ggcacgctgg taagtaactt ctggatgtcg tacatggtcg gcatgtgggc gttcagcttt
    22621 attcttcgct tctttgattt gcaacgtatc ctgatcgtac tggctggtct ggctgcgatt
    22681 ctgatgtacg tctttaacac cggaacaccg gcatatatgg cgtggtcaat tctcgccctg
    22741 ggcttcttct ccagcgcgat ctataccacc atcatcaccc tgggttcaca gcagaccaaa
    22801 gtaccgtcgc caaaactggt taactttgtc ctgacctgcg ggaccatcgg tactatgttg
    22861 acctttgtgg ttaccggccc gattgttgaa catagtggtc cgcaggcggc gctgctgacg
    22921 gcaaacggtc tgtacgctgt cgtctttgtg atgtgcttcc tgttaggttt cgtcagccgt
    22981 caccgtcagc ataacaccct gacctctcat taattgttca tgccgggcgg cactatcgtc
    23041 gtccggcctt ttcctctctt actctgctac gtacatctat ttctataaat ccgttcaatt
    23101 tgtctgtttt ttgcacaaac atgaaatgtc agacaattcc gtgacttaag aaaatttata
    23161 caaatcagc
//
LOCUS       DAZKGF010000075        22615 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZKGF010000075 DAZKGF010000000
VERSION     DAZKGF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.075"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..250)
                     /locus_tag="ACHOQV_002698"
     CDS             complement(<1..250)
                     /locus_tag="ACHOQV_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9631055.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLV"
     gene            complement(247..1917)
                     /gene="menD"
                     /locus_tag="ACHOQV_002699"
     CDS             complement(247..1917)
                     /gene="menD"
                     /locus_tag="ACHOQV_002699"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFP9631056.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     EQHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2006..3301)
                     /gene="menF"
                     /locus_tag="ACHOQV_002700"
     CDS             complement(2006..3301)
                     /gene="menF"
                     /locus_tag="ACHOQV_002700"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFP9631057.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQTFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGEIAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTALNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3380..3685)
                     /gene="elaB"
                     /locus_tag="ACHOQV_002701"
     CDS             complement(3380..3685)
                     /gene="elaB"
                     /locus_tag="ACHOQV_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFP9631058.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3740..4201)
                     /gene="elaA"
                     /locus_tag="ACHOQV_002702"
     CDS             complement(3740..4201)
                     /gene="elaA"
                     /locus_tag="ACHOQV_002702"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9631059.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            4266..5183
                     /gene="rbn"
                     /locus_tag="ACHOQV_002703"
     CDS             4266..5183
                     /gene="rbn"
                     /locus_tag="ACHOQV_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFP9631060.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQALKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCNA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPVTELANDFTVFNV"
     gene            5371..5484
                     /locus_tag="ACHOQV_002704"
                     /pseudo
     CDS             5371..5484
                     /locus_tag="ACHOQV_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
     gene            5539..6510
                     /gene="yfbL"
                     /locus_tag="ACHOQV_002705"
     CDS             5539..6510
                     /gene="yfbL"
                     /locus_tag="ACHOQV_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFP9631061.1"
                     /translation="MKKMTSLCFIPLLLSLTFIIFYQPWVRALPPTPRHASPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFISSGARVTAQDVPITGGPYKNIVADYGP
                     ADGPLIIIGAHYDSVSSYENDQLTYTPGADDNASGVAGLLELARLLQLQTPGIGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            6613..7116
                     /gene="yfbM"
                     /locus_tag="ACHOQV_002706"
     CDS             6613..7116
                     /gene="yfbM"
                     /locus_tag="ACHOQV_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFP9631062.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(7183..8640)
                     /gene="nuoN"
                     /locus_tag="ACHOQV_002707"
     CDS             complement(7183..8640)
                     /gene="nuoN"
                     /locus_tag="ACHOQV_002707"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFP9631063.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(8647..10176)
                     /gene="nuoM"
                     /locus_tag="ACHOQV_002708"
     CDS             complement(8647..10176)
                     /gene="nuoM"
                     /locus_tag="ACHOQV_002708"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFP9631064.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(10340..12181)
                     /gene="nuoL"
                     /locus_tag="ACHOQV_002709"
     CDS             complement(10340..12181)
                     /gene="nuoL"
                     /locus_tag="ACHOQV_002709"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFP9631065.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(12178..12480)
                     /gene="nuoK"
                     /locus_tag="ACHOQV_002710"
     CDS             complement(12178..12480)
                     /gene="nuoK"
                     /locus_tag="ACHOQV_002710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFP9631066.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(12477..13031)
                     /gene="nuoJ"
                     /locus_tag="ACHOQV_002711"
     CDS             complement(12477..13031)
                     /gene="nuoJ"
                     /locus_tag="ACHOQV_002711"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFP9631067.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(13043..13585)
                     /gene="nuoI"
                     /locus_tag="ACHOQV_002712"
     CDS             complement(13043..13585)
                     /gene="nuoI"
                     /locus_tag="ACHOQV_002712"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFP9631068.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(13600..14577)
                     /gene="nuoH"
                     /locus_tag="ACHOQV_002713"
     CDS             complement(13600..14577)
                     /gene="nuoH"
                     /locus_tag="ACHOQV_002713"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFP9631069.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(14574..17300)
                     /gene="nuoG"
                     /locus_tag="ACHOQV_002714"
     CDS             complement(14574..17300)
                     /gene="nuoG"
                     /locus_tag="ACHOQV_002714"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFP9631070.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(17353..18690)
                     /gene="nuoF"
                     /locus_tag="ACHOQV_002715"
     CDS             complement(17353..18690)
                     /gene="nuoF"
                     /locus_tag="ACHOQV_002715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFP9631071.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEVGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(18687..19187)
                     /gene="nuoE"
                     /locus_tag="ACHOQV_002716"
     CDS             complement(18687..19187)
                     /gene="nuoE"
                     /locus_tag="ACHOQV_002716"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFP9631072.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(19190..20992)
                     /gene="nuoC"
                     /locus_tag="ACHOQV_002717"
     CDS             complement(19190..20992)
                     /gene="nuoC"
                     /locus_tag="ACHOQV_002717"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFP9631073.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(21086..21748)
                     /gene="nuoB"
                     /locus_tag="ACHOQV_002718"
     CDS             complement(21086..21748)
                     /gene="nuoB"
                     /locus_tag="ACHOQV_002718"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFP9631074.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(21764..22207)
                     /gene="nuoA"
                     /locus_tag="ACHOQV_002719"
     CDS             complement(21764..22207)
                     /gene="nuoA"
                     /locus_tag="ACHOQV_002719"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFP9631075.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
BASE COUNT         5545 a         6316 c         5828 g         4926 t
ORIGIN      
        1 ccaccagcca gaagttaagg atgttgtaac taactaaggt tttacgcagt aagtcggtga
       61 catcatcaaa tccatcgacg ctaatcgccg ccgaaccacc gtgacctggg agatcaacat
      121 acaaccgtga gtagtcggca aacgcctcgc ccacttcttg ccattcatgg caatcgccgg
      181 aaaaaccgtg caaaaacacc agccagggta aacctggttt accgtgtttt gcctgcgcgt
      241 gcaggatcat aaatggctta cctgcgccag cagttgctgg agggtttgcg caccatcggt
      301 gtcgttaacc accatttcaa ttaccgtggt ggtcggcgtg cgccaggcgt cggcaagtgc
      361 cgtttcaagt tcctgccagt tttgcggacg atgatatttc agctcgaaca tcgcggcggc
      421 gtgctcaaaa tggacgtttt gcggcatcag atagaaacgc tcacgctcgt ttttcggcgt
      481 tggcaacagc gagaaaattt gcccgccgtt gttgttcacc acaattaata ccagcggcgc
      541 ggaaacctga cgtaataacg ccagcgcgtt gagatcgtaa agtgctgaga gatcgcccac
      601 aatcgccagc gtcggtttgc cactggcccg ctgaacgcca gcggcggtcg agagcagccc
      661 gtcgataccg ctggcaccac ggttgctgta caccgggtaa cctgccggaa gttgcgaaag
      721 cgcatcaatc agacgtacca ccaggctgtt accaacaaac aattgaccct gttcaggcag
      781 atagtcgctg atgcgatgcg ccagttgcgc ttcgccaaac gcatcgcgac gggcaataac
      841 cgcctgcatt gcctgttccg ccaggcgcgg aatttcaacg caccagggct ggcgtttttc
      901 tgccggatgc tgctccagcc agtcggcaat attggcaatt aagcgacgtc cacgatggtg
      961 tgccggatca agtcgccctt cgatgtcatc aacaatccag tactcttccg gttcacagct
     1021 tgcctgccat tgcaggagcc gtttgcccgt caggctgctt cccagttgca ccacaatttg
     1081 tgcctgttgc agttcgctgg tcgctttggc attgcctaac cagagatcgg cacacggcag
     1141 cggctgtccg gtttgcgaca gcacgtcgcc aatcagcggc cagccaagag tttgcgccca
     1201 cagtgcaact tttttgccct cttccgcact catgcgcccg gcaaccacca cgccgcgctt
     1261 ttgccgccag aagaaccagt cgcgctgttt ttcactttcc agacgaggcg cttcacgcag
     1321 ccacggtttg tcgtcctgcc accagtcgcc cagccgctgt tgccagctaa gcccggtgtc
     1381 gtccatttcg ccatacagcg gttcagcaaa cgggcagttg atatggactc cgcccgcatg
     1441 aagcgtaccg agagcatggt cgatggtaga aaccagccaa cgtgcaggaa tatcctgagt
     1501 cgggcgcggc agtgaaatac tgtgcgtggg gtgagaggcg aacattcccg gctgacgaat
     1561 cgcctgattc gcgccgcagt caattagctc cggcgggcga tcggcggtta acaggatcag
     1621 tttttctccg gttaacccag cttcaatcag tgccggatag agatttgcta ccgccgtgcc
     1681 ggaggtcaca atcaccgcca ccggctgctt gctgactttc gccagcccca gcgccaaatg
     1741 ccccagtcca cgctcatcga aatgggtgtg gtgaatgaat gcggaattct ccgccgccgc
     1801 taacgttaac ggtgtagaac gcgagccagg ggcgatacag atgtgtctga cgccgtgacg
     1861 cgttaatgct tccagaatga ccgccgccca gcgtcggtta aatgcgctta ctgacatgag
     1921 tttgtccggt atcaatattg cggctaagta taaggagcta caaaaataga ttattgatat
     1981 gaatcggtaa tgatgcgact cattactatt ccatttgtaa taaagtacgc agccctgccg
     2041 ctttgttatc gatttcctgc cactcttgct cggggtcaga accacggaca atgcccgcgc
     2101 cagcatatag ccgcacgaca ttgccactga tttttgctga gcgcagggaa acgcagaatt
     2161 cgctttgttg taaagagaga tagcccgcag aaccggcgta ccattctcgg gtgaacggtt
     2221 cgtgacgggc gataaactgt cgcgccagat cgcgcggtaa gccagcaact gctgccgttg
     2281 gctgcaactg atgtaaacag atcacatcat ccgctttgtt gagtgcagtc cagatacagc
     2341 ggcgaagatg ctgcacttta cgcagacgca gtacctgcgg cggtaaaaca tccaacgtct
     2401 gggtatcggc ctgcaatcgt tggcagatat cctccaccac caacatgttc tcgcgctggt
     2461 ttttatcatc cgccatcagc cactcaccta actgctgcgc ctgcttatca tcaggatgat
     2521 ttgctaccgt tcccgccagc gcttcagtac gcagcgcttt gtcacgccgc cgccataacc
     2581 gttccggtga agagccaaga aaagcaattt cgccatcaaa ggccatgtaa aaatggtagc
     2641 aattcagatt cagtcgacga ctggcagcca tcatcgccgc cgcattgacc ggacttgcga
     2701 aatgcaggtc agttgcccga gcaagcacca ctttgtcgag ctcaccttcg gcgatggttt
     2761 tcgttgccag ttcgattaat tgcgtccagc ccgttttgtc cggccagtgt tgttctcggg
     2821 tggtggttaa atgtaaccca ggcaagggtt taatactcac cagtgtggcg ataaattctt
     2881 ttgccttaat cgcatcatgc tgaagggagc tttcgctgaa tagcgtcagc cgcagcgtgg
     2941 ctttaccgcc acagcgtcgc cattcgaggc gtggtaaaag taaattgccc tgcgacgggt
     3001 caaacgcatt tagcccccag atgcgtaagt cggcgtgttc cgggtgctga cgaagaaacg
     3061 tttgtgcctg gtccaacgac gtaaaacggg taatcgcgcc caggacggca gcttcttcat
     3121 caccattacg ttgttgccag tagaattgcg ggtacgtttg ctgactggcc agccagctca
     3181 aggcatcaaa agcgtctttg agagggaaag gaatatcaat aacccgaatg ccgggtgtcg
     3241 ccggaatctc ttgcgacaaa tggcgcagta gattttccag cgccgtagta agtgattgca
     3301 cgcggacctc tccctgttaa aagcctcaca ttatacgggg tacgacaaaa aaaatgcagt
     3361 accccggtgt agggaggttt taacggcgtg ccagcaacag tcctagtacc agcccaacgg
     3421 ccgcgcccac accaattcct tgccagggtt tttcgtggac gtagtcatca gcacgataaa
     3481 cagcctgctt cgcccgatag taataactgt ctgaagcctg gctaacccgt tttttcacat
     3541 catccagcgc tttttctgca cgcgctttca gctcaacata tttctgatcg gcgggatcgc
     3601 cagaggagcg gagcacctct tccagtgttt cactaagcag cgtcaggtca tcatcaatac
     3661 gtgtatcacc aaactgatta gacattctcg ttctccattt gtgtaaaacc tgtcactaac
     3721 tatagacaag ggttattgat tacgcctgaa ttacctcgcg tgccatccca atgtgtggga
     3781 taccatcttc ttcataaata tcggtcacag ggatgaaacc aaattgccca taaaagtttt
     3841 gtaaatgcgc ctgcgccccc agataaatcg gcttctccgg ccagcggcga gtacacgatt
     3901 ccagcgcctt actcatcaat tgctgaccga ttttttcacc gcgtaaggct tcgctgacaa
     3961 tcacccgacc aatcacaacg ggttgcagtt cgtcgtcact tttcagaatc ctcgcatacg
     4021 ccaccagtgt cccgttatgc caaccgagaa tgtggcgatt ttctccctcc agatcgtcac
     4081 catcgatatc ctggtaagga cagttttgtt ccactacaaa taccgcacaa cgcagttgta
     4141 ataaggcata aagctgagaa acggataaat cagaatgatg cagatcttgc cagtcgatca
     4201 tggaaggctc ctttgtgctg tttgctttat actagccgga atattttcag cagcgtgatg
     4261 aactcatgga attaattttt ttaggtactt cagccggtgt gccaacccgt acgcgcaatg
     4321 tcacggcaat attacttaac ctgcaacatc cgacccagag cggtctttgg ttgtttgact
     4381 gcggtgaagg cacccagcat cagctactgc ataccgcctt taaccctgga aaactggaca
     4441 agatttttat cagtcacctt catggcgatc atctctttgg tttacccggc ttgctgtgca
     4501 gtcgttctat gtcaggcatt atccaaccct taacgattta tggtccgcac ggtatccgtg
     4561 aatttgtgga gaccgcgctg cggattagcg gctcatggac cgattatccg ctggaaattg
     4621 tcgaaattgg cgctggcgaa attttcgatg atggcctgcg caaagtgacc gcttatccgc
     4681 tggaacaccc actggaatgc tatggctatc gtattgaaga acatgataaa ccgggtgcat
     4741 taaatgctca ggcattaaaa gctgctggcg tgccgcctgg cccactgttt caggcattaa
     4801 aagcgggcaa aacaatcacg ctggaagacg gaaggcagat taacggcgca gattacttag
     4861 ccgctccagt gccaggtaaa gcgctcgcta ttttcggcga taccggcccc tgcaatgccg
     4921 cacttgacct ggctaaaggt gtcgatgtca tggtgcacga agcgacgctg gatataacca
     4981 tggaagccaa agccaatagt cgcggtcata gctctacacg ccaggctgcg acactggccc
     5041 gtgaggctgg agtcggtaag ctaatcatta cccacgtcag ctcgcgctat gatgacaaag
     5101 ggtgtcagca cctgttacgt gaatgcaggt caattttccc ggtgactgaa ctagcgaatg
     5161 atttcaccgt gtttaacgtt taactctgct tattattaaa aacagggcga aacttgccct
     5221 gttatcgcaa cccgcgctac atatatccac ccacacgggt atattgagcg atttttgata
     5281 tttattcatc tcctcacgca ctatattcag agcaataagt tgcctctttt tatcttcatt
     5341 cagcacaacc tttgcaacta aaaggagcct atgatggtta cagttgtcag caattattgt
     5401 caattatctc aaacccaact ctgtcagaca tttgcagaaa aatttactgt gaccgaggaa
     5461 ttactgcagt ctttaaaaaa acacttataa ctcattacaa ataatgatga aataacgctt
     5521 tttattgata atttacatat gaaaaaaatg acatctcttt gttttatacc attactacta
     5581 tcgcttactt ttattatttt ttaccaaccc tgggtcaggg cattaccacc aacgccccga
     5641 cacgctagtc ctgagcaatt agaaaaaaca gttcgttatc ttacacaaac tgtgcatcca
     5701 cgtagcgccg acaatattga taatctgaat aggtccgcag agtatataaa agaggtcttt
     5761 atcagtagcg gtgccagagt taccgcgcag gatgtcccca ttacgggtgg cccctacaaa
     5821 aacattgttg ctgattatgg tcctgccgat ggaccgctga ttattattgg tgcgcactat
     5881 gactctgtca gtagttatga aaacgatcaa ttgacctata cgccgggcgc ggatgataac
     5941 gccagcggcg tggcaggatt actcgaactg gcgcgtttgt tacagctgca gactccaggg
     6001 attggcgtcc aactggtcgc ctatgcgtcg gaagaaccgc ccttctttcg tagcgatgaa
     6061 atggggagcg cggtacatgc agcttcgctt gagcgtccag tgaaattaat gatagcgctg
     6121 gagatgattg gttattacga ctcaacgcct ggtagccagg attacccatc cccggcaatg
     6181 tcctggcttt atcccgatcg gggagacttt attgccgtgg tcggcagaat gcaggatatc
     6241 aacgccgttc gtcaggtaaa agcggcattg ttgtcatccc gggatttatc tgtttattct
     6301 atgaatgccc caggttttat tcccggtatt gatttctctg atcaccttaa ttattggcaa
     6361 cacgatattc ccgccgtaat gattactgac accgcttttt atcgtaataa acaataccac
     6421 ttgcccggtg atatcgcaga cagattaaat tatcagaaaa tggctcaggt agtagatgga
     6481 gttacaacct tgttatacaa cagtaaataa taaaaatttc tcgaggcgga tccagaaaaa
     6541 gcaacatgcg cattttattt tctttcgtct gttttaagat gcgaaacatg aaattcaacg
     6601 gaggaaaaat aaatgggaat gattggctat tttgcagaaa ttgactcaga aaagattaac
     6661 caactgcttg agtctactaa aaaacctttg atggataata tacatgacac gctttcagga
     6721 ttacgccgac tggatattga taaacgctgg gattttttac actttggcct gacgggtaca
     6781 tctgcatttg accccgcgaa gaatgacccg ctaagtcgcg cagtgcttgg tgaacacagc
     6841 ctagaagatg gcatagatgg cttcctggga ttgacctgga atcaggagtt agccgcgaca
     6901 atcgaccgac tggaagctct ggatcgaagt gagctgcgta aacagttttc cattaagcga
     6961 ctcaatgaaa tggagattta tccgggagta accttttcag aagagttaga ggggcaactc
     7021 tttgcttcga tcatgcttga tatggaaaag ctgattagcg cgtatcgacg tatgctgcga
     7081 caagggaatc atgcgctgac ggttattgtt gggtgatggt gtcagggcac caaaaaaaac
     7141 cgccgaacat gtcggcggct ttctgactta caaactgact gcttacatca gcggcattgc
     7201 taaccgcaca atgctaatca gcggttgtgg ccatacaccc agcaccagta ccaacagtgc
     7261 agagatcagc accacgatac cgcctgcgct gtactgccag tttgatggtg catcgcgacc
     7321 cggttgttcc ggagcgtgaa ggtataagct caccgccacg cgcaggtagt agtagaggcc
     7381 gattgccgaa ccgacaacta cggcacccac cagccaccac aagtgtgcct ggacaccgac
     7441 cgccagcacg tagaacttac cgataaagcc cagcgtcatt gggataccag ccagtgaaag
     7501 catcatcacc gtcatcactg ccgcgaggat cggacgatgc cagaacagac cgcggtaaga
     7561 gaacagggaa tcagcatccg ggccacgata cgggctggac atcaggctga ccacgccgaa
     7621 cgcgccgagg ctgctgaaca gataacctgc caggtaaacc cctaccgctt ccatcgacat
     7681 ctcgccagtt tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag agatagatga
     7741 gtaaccgagc agacgtttga tattggtctg gctcagcgcc atcaggttac cgaagatgat
     7801 ggaggcaaag gcgataatcg ccagcaccac gcgaatcgct tcgctgtcac ccaccggtgc
     7861 gtagaggaac agacgcatca ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa
     7921 agtggaaacc ggcgcaggcg cgccctggta tacgtctggc gtccacaggt ggaacggcac
     7981 cagagagagt ttgaagccga ggccgacaat catcaggccg aaacctgcca gcaacagcgg
     8041 ctcgttgagc ataccgtcgc cgaggttttt acccagcgcg acaaacgaca ggtcgccaga
     8101 ctgcgcatac accagcgcca taccgaacag caggaaagaa gacgctgcgg cagagagaat
     8161 ggtgtatttg atactggctt ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc
     8221 aaacagcggc aaagagatca gttcgatacc gaggaacaga gacgccagat ggttggcatt
     8281 cgccagcagg atcccgccca gggcggcaat taacaccaac aggtagaact cgtccttgtt
     8341 gtcgttatag ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc tcgccaacaa
     8401 taccagcccg gtgtaaagca tggcgaaacc atcaacgcgc atcagtggcg taacgtccat
     8461 agcgcccgcc tggccgacaa accagagcga aaccagcgcc gcgttaagcc caataaccga
     8521 gagcgtagcg ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca ccaccaccac
     8581 cgtcaagccg acgatcagca acggtagcag tgcgatcagg ttttgtggag ttattgtcat
     8641 ggcgatttac ggccttgtag tagtaacgga attaacaaac cactgctgga tattgccaat
     8701 cgcggagtgc gaggtatcca gaatcggctg cggatagaag cccagcagta ccagcagcac
     8761 caccagcaac aggatcataa acagctcacg cagcgacatc cctggcagtt cctggctggc
     8821 aatctggctt ttcgctttac cgaagtaagc gcgatgtaac atcgccagcg aataaacaga
     8881 tgcaaagacc agcccaaagg tagagataac ggtaatcacc gggacaacct ggaagctgcc
     8941 gaacagaatc ataaattcgc cgacgaagtt accggtgcca ggcatcccaa gcgttgccac
     9001 cgcaaagaac agcgacagtg ctggcagcca tttcatcttg ctccacagac cgcccatcat
     9061 gcgcatgtcg cgggtatgga tacgttcata aagctgaccg cagagaataa acagacccgc
     9121 cgccgacaag ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca actggctgcc
     9181 agtgtagata gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg
     9241 tttgatatcg gtctgggcga aggccatcca cgcaccgtag aagatgccga taacacccag
     9301 ccacatagcg attggcgcga actctgccga cgcgttcggg aacaacggta gggagaaacg
     9361 cagcagaccg taagcggcgg ttttcagcaa gatccccgcg aggtcaacgg aaccggcggt
     9421 tggagcctgt gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg gcattttcac
     9481 cgcaaatgcg atgaagaagc ccagcatcaa cagatattcc acaccactgg acattggcgt
     9541 attcaacagc tcttcatagt tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac
     9601 cagcgccagg atggcgatca acatcaccag accactcgcc tgggtgtaaa tgaagaactt
     9661 ggttgccgcc gtgatacgcg ttttaccgtc agaggcttta tgcccccaca gtgcgatcag
     9721 gaagtacatc ggcaccagca tcatttccca gaagaagaag aacaggaaca tgtcgatggc
     9781 aaggaacacg ccgataacgc cgcccaggat ccacatcagg ttgaggtgga agaagccctg
     9841 atatttttcg atctctttcc acgaacagag taccgccagc acaccgagca gaccggtcag
     9901 cacgaccatc agcagcgaca gcccgtcaat ggcgagatgg atagagatac caaaacgcgg
     9961 gatccacggc atgtcgaatt cagactgcca ctgcggaatt ccggcggatt gcgtcagtga
    10021 ataaccgccc tgcaaccaca gttgcagcga cagcgccagc gtcaatccca tggtgatcag
    10081 cgcgatccag cgcggcacct tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc
    10141 gataaaggga attaatatta gccagggtag taacatggcg atctttattc cttgtaaaag
    10201 tcccgtcagg accggatttt caacgaattc tcacgacaga aaattctctt cggactgggg
    10261 gtagtgtcgg atgcaccgcg aatgcggctt atccgaccta cgttcttata tgcccagggg
    10321 caatcccaca atccttaact caacgcagta ccatcaacag tgccagcacc acgaccgcac
    10381 cgatgctcat ggatgccaca taccagcgca gataaccgtt ctcgcttaac agcagacctt
    10441 tacctgcaaa gcgggaaagg acagccggga tgttcatcat tgagttcagc ggatcgcgtt
    10501 tcagcaacca ggcaataccc aggaacggct tgacgaacac tttgtcgtac agccagtcaa
    10561 agccccaggc gttgtaccac caggtaccca gcagacggcc cggcgcactg ttggcgatgg
    10621 aggtcaccag agtacgttta cccagccaca gccaggcagc cagcagaatg ccgaccaccg
    10681 cgaccacgcc agaggtaatt tccagggtca acatgctgcc gtgcgccagt tccgtcgttt
    10741 gcggaagcac gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca
    10801 gcagcacaat cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt
    10861 cttttccgtg gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac
    10921 cgaccagacc tgccaccatc agattgatat gaccattcgc catcgcaccc gcgaggatct
    10981 catccttact gaagaagccc gcagtgacca gcggcagtgc cgacagtgct gcgccgccca
    11041 ccaggaagca gagataaacc agcggaatag atttacgcag accgcccatc ttgaagatgt
    11101 tctgttcgtg atggcaggcc agaatgacgg aaccggatgc caggaacagc agcgctttaa
    11161 agaacgcgtg ggtcatcaag tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga
    11221 ggaacatgta gccaatctgg ctcatggtag agtaagcgag aacacgtttg atatcggtct
    11281 gtaccagcgc ggcaaaaccg gccagcagca gcgtaaccgc cccgacaatg cccaccagat
    11341 gcagaacttc cggcgtcatc aggaacaggc cgtgggtacg agcgatcagg tagacacccg
    11401 cggttaccat cgttgcggcg tggatcagcg cggagacagg cgtcgggccc gccatcgcgt
    11461 cggcaagcca tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca
    11521 tcagcgtcgc ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca
    11581 ccatttcgcg gaagttcagg gtgcccagtt cgttgtaaag aatgaacagc gcgaaagcga
    11641 ggaacacgtc acccacacgg gtcacgacga acgctttcat tgccgctgcg ccattcttcg
    11701 gatcggtgta atagaaaccg atcagcagat aggagcacag gcccacgcct tcccagccga
    11761 ggtacatcaa cagtaggtta tcggcgagaa ccagaactac catgctggcg atgaacaggt
    11821 tggtgtaagc gaagaagcga gagtagccct cttctccgcg catataccag gaggcataca
    11881 tgtgaataag gaaacccaca ccagtgacca ctgagagcat ggtcagcgac aggccgtcca
    11941 gcaccagatt gaaaccaatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc
    12001 tgtatgcctg ctcaccgtta gcgaagaagt caacgccgat aaaggcggtt accagcgccg
    12061 ccaggcccac agagccaacg ccgacgatcg ccgagacgtt ttcagaccag cgcccacggg
    12121 agaacgccag caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca
    12181 tccgcgcatc tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag
    12241 cagcagcgca aggccgatac tcgcttccgc cgccgcgagg ctgatggcga gaatgtacat
    12301 cacctgaccg tcggtctggc cccagtagct tccggcgacc acaaaggcca gcgcagaggc
    12361 gttaatcatg atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc
    12421 ggttaagcca agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat
    12481 gcgtgctcct ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc
    12541 gcacgctctt cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa
    12601 gccagttcca ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg
    12661 attggcgtac cgtcgatacc ctgatcatta acaccaagga tggcgtaaac aatcaccacc
    12721 agcatgatgg ccgacaaaat cgccggacca atccacacct gcggtttcag ccactggcgt
    12781 tcctgttcga tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc
    12841 ataatggcac ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc gcccagtgag
    12901 aagaacaccc cagaaatcgc cagcagcgaa ataatcaggt acagcagcgc gtgtaccgga
    12961 ttggtatggg tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa
    13021 gcgaactcca ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt
    13081 cgttctctgc ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt
    13141 tatattccgg gtatttgccc ggaccggaga tcagcagatc ctctttctcg taaaccagat
    13201 cctggcgctt gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac
    13261 aggcttcttc gcacagacca cagaaaatgc agcgtgagaa gttgatgcgg aaaaattccg
    13321 gataccagcg accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg
    13381 ctaccgcgca gaggttacag gctacgcaac gctcttcgcc gtccggatcg cgggtcagaa
    13441 cgatacgacc acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt
    13501 cgcgtttggc gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac
    13561 ctactaacaa ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat
    13621 gacagccgcc gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa
    13681 ggacattacc tggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat
    13741 cataaagaac gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca
    13801 gccaccgaag aacagcgtca ccatcagtgc agagatggtc acaatcccga tgtattcgcc
    13861 cacgaagaac agaccgaact tcatgccgga atattcaatg tggtaaccat ccgccagttc
    13921 ctgctcggct tccggctggt caaacgggtg acggtgacac accgccacgc ccgcgatggc
    13981 aaaggtaata aaaccaaaga attgcgggat gacgttccac acatgcgcct ggctgttgac
    14041 gatgtcggtc atgttgaatg aaccggcctg cgccaccacg cccatcaggg aaagcccgag
    14101 gaacacttcg tagctcaggg tctgcgcaga agcacgcatc gcacccagca gcgagtattt
    14161 gttgttactt gaccagcccg caaacagcac cgcgtaaacc gccagaccgg ccatcatcag
    14221 gaagaacaaa atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat
    14281 cgcaaaggcc agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg
    14341 atccgagaat ttcgggatcc agtcttcttt aaagaacatc ttgatcatgt ccgcgaccag
    14401 ctggagcgaa ccgccccagc caacacggtt aggtccgtaa cggttctgga acagacccag
    14461 caggcgacgt tcgccaaagc tcatgaatgc cccacaggtg acaaccacca gcaggatcac
    14521 caccgctttg aggatggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt
    14581 tgtgcctcct tgagatcctc aagatgcgcg ccagccagca ccggagcaat gccggacata
    14641 cccatcggca agcccacctg ccctgccgtc agtccttcgg cgatttcaac cggcagcgtg
    14701 accgtgttgc catcgtaact aaaggagacg cgtgtacccg cgttcacacc caacttcgcg
    14761 gcatccgctg ggttgagttt gatgtacggc tgcggcatac ggctctggaa gaccggagca
    14821 cgctgtgaca attcatcgct accaaacagg tggtagtacg gcgcgatacg ccatttcccg
    14881 tcctgcggct ggaagcgtgc cggtacgctg gtgaagtaat ccagaccatt ttcgctggtt
    14941 tcaaacagac gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc gtcctggaat
    15001 ttgttccacg cctgcgggga gttccagcct ggcgcccagg caaacggcac ttgcgaacgg
    15061 tgcgcggtcg gctggttgtt accttccatc gagaaggtga acatggtgtc aatatcctgc
    15121 ggctgacgcg gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg
    15181 tgcggttcac gggccagttt ctgcccacga atacggaatg tcgcatccgg cgcagcatct
    15241 ttgatacccg ccagttccgg gattttcgcc acaacagcgt caatcacatg gtcgagctgc
    15301 gtccagtcca cttcacggct cagcaaggtg ctgtgcagcg agtgtaacca acgccagctt
    15361 tccagcatga cagttttgct gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg
    15421 gcacggcctt cgttgttaat caccgtaccg tcgctttcag caaagctggc agcagaaagt
    15481 accagatggg cgttttccat aatcgctgtg cgttgatggt caaccaccat caccagcggc
    15541 gctttagcca gcgcagcatt cacgcgggta gcggaagcgt gacgatgcag atcgttttcc
    15601 agcaccacca ccgcgtcggc gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgat
    15661 ccaccgccca taatgcccag ccccatgctg ttgacggaac gggcaatcat ggtgataccg
    15721 acgtcagcgc cgcgaccttt cagggctttc gccacgttag ccgcagcctg aatcacttca
    15781 gcgctaccgg cgttcgtccc ggagataatc aacggtttct tcgcacctgc cagtgcctgc
    15841 acgatgacgt cgattttgct ttgcagctca ggttcaatac cgtcaaccgc tggcgcggag
    15901 ttatccagcg catgggcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg
    15961 taagtccacg ccgcgatatc atccagacgg gtgtcatcaa cgttggtaac aaacagcgga
    16021 tgcttcgcac gttggccgat gttgaggatt gccgcaatct gccagtcagc cactttctgt
    16081 gctgccgcca tttcgcgcgc tttacctttc accgcctgac gaactgccag cgcgacgcgc
    16141 gcgccggtct gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg
    16201 cgcagtgccg gcgtataaat gccgccttca cgcagcactt tcagcgccag ttgcagacgt
    16261 tcctgctcac cgtgagcgat accggtgtag aagttttctt cgcccaccag ctcacgcagc
    16321 gcaaagttgc tttccacgct ggcacgcgga gaaccaatgc cgatcacttt cttcgactga
    16381 cgcagaatat ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg
    16441 ccacgacgct gtactggctg acgcggacga tctttcaggt tgacgtaacc gtaaccgaaa
    16501 cgaccacggt cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga
    16561 cgcagttcgc cgtaacgctc gccggggctg atgttacagc cgatggaaca ttgctggcag
    16621 atgctcggcg caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg
    16681 gtgaatacgc cggtcgggca aatctcgacc agattaccgg aaaattcgct ttccagcgtg
    16741 ccgtcttccg ggcgaccgaa gtagacgttg tcgtgcgcac cgtaaacgcc caggtccgtg
    16801 ccgtcagcgt aatctttgta gtaacgcacg cagcggtagc aggcgatgca gcggttcatt
    16861 tcgtgagaga tgaatggccc caaatcctga ttacggtggg tacgtttggt gaaacggtag
    16921 cgacggaagc tgtgtccagt catcacagtc atatcctgaa gatggcagtt accgccctct
    16981 tcacataccg gacagtcgtg cgggtggttg gtcattaacc actcgaccac actttcacgg
    17041 aactgtttcg cttcttcatc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata
    17101 caggacatca ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac
    17161 tggcggcaag caccgacgct tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg
    17221 cccagagaca gacaagcttc cagcaagttg tccgctccgt tgacctcgta ttctttgccg
    17281 tctacatgaa ttgtagccat tagcatgctt ccagttttct cagtcagaga ctgagcgtta
    17341 atcgaaattc ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg
    17401 ggtattgctg aacggctgtt tgattcccgc ctcaaattct tcgcggaaat atttgatggc
    17461 gctctgtaac ggctccactg caccaggtgc gtgggcacag aaagttttac ccgggcctaa
    17521 gaatcgacac agttgctcaa gtgtttcgat atcgcccggc tgaccttcgc cacgctccag
    17581 cgcacgcaga attttcacgc tccacggcag accgtcgcgg cacggcgtac accagccaca
    17641 ggactcacgg gcgaaaaact cttccaggtt acgcaccagc gacaccatgt tgatctcatg
    17701 gtcaaccgcc atcgccagcg ccgtacccag acggcttccc gctttaccga tactttcgaa
    17761 ttccatcggc agatcaaggt gcgcttcggt caggaagtcg gtgccagcgc cgcctggctg
    17821 ccaggctttg aatttcagac cgtcacgcat accaccggcg taatcttcga ggatctcgcg
    17881 tgcggtggtg ccgaatggca gttcccacag gcccggattt ttcacccgac cggagaagcc
    17941 catcagcttg gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt
    18001 agcgaggatc gccggaacgt tacacagggt ttcgacgtta ttgacacagg tcggtttgcc
    18061 ccatgcaccg gaggttgccg ggaatggagg cttcgagcga gggttagcgc gacgcccttc
    18121 cagggagttg attaacgctg tttcttcacc gcagatgtag cgcccagccc cggtatggac
    18181 gaacaattcg aagtcgaaac ctgttcccat aatgtttttg ccaagcagac ccacttcggt
    18241 ggcttcggca atggcacggc gcagattcac tgccgcttcg atatactcgc cacgcaggaa
    18301 gatgtagcca cggtacgctt tcagcgcaaa cgcggagatg agcatacctt ccaccagcag
    18361 gtgcggcagt tgttccatca gcaggcggtc tttataggtg cccggctcca tttcatcggc
    18421 gttacacagc aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccatttcag
    18481 gccggtagaa aagcccgcgc cgccgcgccc tttcagacca gcgtctttta cctgattaac
    18541 gatttcgtcc ggagacagcc cggtcagcgc cttacgcgcg ccttcgtaac cgtttttgct
    18601 gcgatattcg tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg
    18661 cgtttcggga gtacggataa tgtttttcat ttataccgct ccagcagttc agggatcgct
    18721 tccggggtca gatgcgcgtg agtgtcctca tcgatcatca tgtttggccc tttgtcgcag
    18781 ttccccaggc agcaagttgg cagcagcgta aagcggccat caaatgtcgt ttgccctggt
    18841 ttgatgttca gctttttctc cagcgccgcc tgaataccct ggtaaccgtt gatgtgacag
    18901 accacgctgt cgcaataacg gatcacatgg cgaccaaccg gctggcggaa gatctggcta
    18961 tagaacgttg ccacgccttc gacgtcgctc gccggaatac ccagcacatc ggcgatcgcg
    19021 tggatcgcgc catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg
    19081 gacgccgcac gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc acgctctgcc
    19141 gcactcagct caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca
    19201 catctgacat aacaaaatcg atactgccca gataaacaat caggtcagac accaggctgc
    19261 cgcggatcgc cgccggaatt tgctgcaaat gcgcataact cggcgtacgg atacgggtgc
    19321 gataactcat ggtgctaccg tcgctggtca ggtagtaact gttgatcccc ttggtcgcct
    19381 caatcatctg gaaagattca ttggctggca tcaccggacc ccacgacact tgcaggaagt
    19441 gggtgatcag ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg
    19501 gatgatccgc tttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca
    19561 gactctggcg cagctcttcc actttcagca ttacgcgggt gtagcagtca gaaacaccac
    19621 cacccaccgg gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca
    19681 cgtcgaagtc gatcccggtg gcgcgcaggc ccgcgccagt ggtgccccac tccagcgcct
    19741 ctttcgcgcc ataggcggca acgccctggg aacgaccttt cagaatggtg ttttgcagcg
    19801 ccgctttctc gtaagacgcc agacgtttcg gcatccagtc gaggaactca cgcagcaggc
    19861 gatcccagcc acgcggcagg tcgtgcgcta caccgccaat acggaaccac gccgggtgca
    19921 tacgaaaacc cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga
    19981 agaacactgg cgtcatcgcg ccgacgtcct gaataaaggt ggagatatac agcaggtgac
    20041 tgttgatgcg gaacagttcg gagagcatta cgcgaatgac gttaacgcga tccggcacgg
    20101 tgatcccggc cagtttctct accgccagca cgtaaggcat ttcgttaacg cagccgccga
    20161 ggtattcgat acggtcggta tacggaatgt agctgtgcca ggactggcgt tcgcccattt
    20221 tctccgcacc acggtggtgg tagccgatgt ctggtacgca gtcgacaatt tcttcgccat
    20281 caagctgcaa aacgatgcgg aaagccccgt gcgccgacgg gtggttcggg ccgaggttga
    20341 ggaacatgaa gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg
    20401 tcagggcttc catctccagg tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg
    20461 tagcgcgcgc cggataatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga
    20521 tgcggcgcag gttcgggtga ccgtcgaaag taatgccaaa cagatcccag gtttcacgct
    20581 cataccagtt agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg
    20641 ccagcgccac cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa
    20701 cggaaaaatc cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt
    20761 gtaagtcaaa cagcatgacg taaggtttcg gcagtttctt taagaaatcg cccacttcca
    20821 gtaattgttc acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa
    20881 aggcatccgg cccaaaacgg ttgcgcagtt cgccaatcac cggatcatca agatgatcgc
    20941 gggtctgcca ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg
    21001 gtccgtggtg actgtcggcg cagtaagctt cgcgaaatag atttaggaat aagcgaagaa
    21061 aaattccttt gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg
    21121 caatgcgttc accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct
    21181 gatcgccaac cacccaggag agcggacgac gttctttacc gatagattcc tgcaacagca
    21241 tcagtgcctg catgtacgct tcaggacgcg gagggcagcc cgggatatac acatcaaccg
    21301 ggatgaattt atcgacgccc tgcacaacgg aataaatatc gtacataccg ccggagttgg
    21361 cacaggcacc cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa
    21421 taaccggtgc catttttgta aagcaggttc ctgcaacgac catcaggtca gcctgacgcg
    21481 gcgaagctcg caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag
    21541 tcaccatctc aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac
    21601 gaccccagtt aaccatgtca ttgagcttgc ccataaacac gtttttatta acttcttgct
    21661 ccagagggtc ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg
    21721 gatctatgcg ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag
    21781 cgatactgtt cgtttccggg ttcatacgct cgcggcgtga acgcgcgggc gtccagtcca
    21841 gcgcgccaat acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag
    21901 cttccacaaa gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca
    21961 gcgcttcaac gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc
    22021 gtaagcgggc ggagccgacc gagtcgatac cggattcaaa cggaacgttt ttcgaccttg
    22081 cgcgtgcgcg accgcctaaa aaccaaccgc ctaccagcat caggcaacac aggccaatgg
    22141 caacgataag aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca
    22201 tactcattgc ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac
    22261 gccacatcga ttcatgggga ggaataaaaa aaaccttaca atcactgtag aaattctttt
    22321 atacagctaa ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa
    22381 agtttcttca cattagttta cataatatca acaccattag catttaatgc cctttcaccc
    22441 cagatccttg acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa
    22501 tggaaaaaat acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga
    22561 gtattttctt gatctgtgac acgcttttgt cattccataa caaaaacgca gcaac
//
LOCUS       DAZKGF010000076        22435 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZKGF010000076 DAZKGF010000000
VERSION     DAZKGF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.076"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            138..1835
                     /gene="ypdA"
                     /locus_tag="ACHOQV_002720"
     CDS             138..1835
                     /gene="ypdA"
                     /locus_tag="ACHOQV_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFP9631076.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1850..2584
                     /gene="ypdB"
                     /locus_tag="ACHOQV_002721"
     CDS             1850..2584
                     /gene="ypdB"
                     /locus_tag="ACHOQV_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFP9631077.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2597..3454
                     /locus_tag="ACHOQV_002722"
     CDS             2597..3454
                     /locus_tag="ACHOQV_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9631078.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPVRQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3457..5952)
                     /gene="ptsP"
                     /locus_tag="ACHOQV_002723"
     CDS             complement(3457..5952)
                     /gene="ptsP"
                     /locus_tag="ACHOQV_002723"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9631079.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDGCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLNLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQMLADKRQQQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5977..7014)
                     /gene="ypdE"
                     /locus_tag="ACHOQV_002724"
     CDS             complement(5977..7014)
                     /gene="ypdE"
                     /locus_tag="ACHOQV_002724"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9631080.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSTIRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(7014..8099)
                     /gene="ypdF"
                     /locus_tag="ACHOQV_002725"
     CDS             complement(7014..8099)
                     /gene="ypdF"
                     /locus_tag="ACHOQV_002725"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9631081.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVICR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIFADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQLKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGVEVLYAMPKTVLLTGEA"
     gene            complement(8114..9361)
                     /locus_tag="ACHOQV_002726"
     CDS             complement(8114..9361)
                     /locus_tag="ACHOQV_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9631082.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFSAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9383..9709)
                     /locus_tag="ACHOQV_002727"
     CDS             complement(9383..9709)
                     /locus_tag="ACHOQV_002727"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP9631083.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9928..10893)
                     /gene="glk"
                     /locus_tag="ACHOQV_002728"
     CDS             complement(9928..10893)
                     /gene="glk"
                     /locus_tag="ACHOQV_002728"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFP9631084.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKIEVQDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11097..12353
                     /locus_tag="ACHOQV_002729"
     CDS             11097..12353
                     /locus_tag="ACHOQV_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFP9631085.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     QMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVMGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12468..12794
                     /gene="ypeC"
                     /locus_tag="ACHOQV_002730"
     CDS             12468..12794
                     /gene="ypeC"
                     /locus_tag="ACHOQV_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFP9631086.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12935..14173)
                     /locus_tag="ACHOQV_002731"
     CDS             complement(12935..14173)
                     /locus_tag="ACHOQV_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP9631087.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14509..15711
                     /gene="nupC"
                     /locus_tag="ACHOQV_002732"
     CDS             14509..15711
                     /gene="nupC"
                     /locus_tag="ACHOQV_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFP9631088.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15760..17949)
                     /locus_tag="ACHOQV_002733"
     CDS             complement(15760..17949)
                     /locus_tag="ACHOQV_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP9631089.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLARMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18157..18232)
                     /locus_tag="ACHOQV_002734"
     tRNA            complement(18157..18232)
                     /locus_tag="ACHOQV_002734"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18197..18199),aa:Ala,seq:ggc)
     gene            complement(18272..18347)
                     /locus_tag="ACHOQV_002735"
     tRNA            complement(18272..18347)
                     /locus_tag="ACHOQV_002735"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18312..18314),aa:Ala,seq:ggc)
     gene            18568..18927
                     /locus_tag="ACHOQV_002736"
     CDS             18568..18927
                     /locus_tag="ACHOQV_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP9631090.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18929..19321
                     /locus_tag="ACHOQV_002737"
     CDS             18929..19321
                     /locus_tag="ACHOQV_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003834896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP9631091.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESE"
     gene            complement(19372..20787)
                     /gene="gltX"
                     /locus_tag="ACHOQV_002738"
     CDS             complement(19372..20787)
                     /gene="gltX"
                     /locus_tag="ACHOQV_002738"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP9631092.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            21046..21121
                     /locus_tag="ACHOQV_002739"
     tRNA            21046..21121
                     /locus_tag="ACHOQV_002739"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21079..21081,aa:Val,seq:tac)
     gene            21166..21241
                     /locus_tag="ACHOQV_002740"
     tRNA            21166..21241
                     /locus_tag="ACHOQV_002740"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21199..21201,aa:Val,seq:tac)
     gene            21288..21363
                     /locus_tag="ACHOQV_002741"
     tRNA            21288..21363
                     /locus_tag="ACHOQV_002741"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21321..21323,aa:Val,seq:tac)
     gene            21368..21443
                     /locus_tag="ACHOQV_002742"
     tRNA            21368..21443
                     /locus_tag="ACHOQV_002742"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21401..21403,aa:Lys,seq:ttt)
     gene            complement(21554..>22435)
                     /gene="xapR"
                     /locus_tag="ACHOQV_002743"
     CDS             complement(21554..>22435)
                     /gene="xapR"
                     /locus_tag="ACHOQV_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFP9631093.1"
                     /translation="ERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKEL
                     ENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELGV
                     VGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPTG
                     FTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQVG
                     FSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVYE
                     TQQVTPALVKLLAALAQ"
BASE COUNT         5519 a         6012 c         5581 g         5323 t
ORIGIN      
        1 tcaagagatg cattgctaat acaccattta tctatcaatc cccttttaaa attgtggttt
       61 ttcctcaaaa acggcctgct ggtcatcagc cttcaggtta cctatcatag aggtttaatc
      121 cttattcaga gtcacccgtg cacgaaatat tcaacatgct gctggcggtc ttcgatcggg
      181 cagcgttaat gcttatctgc ctgttctttc tcatccgtat ccgtctgttt cgcgaactgt
      241 tgcacaagtc ggcgcactcc ccaaaagagt tgctcgccgt taccgccatt ttctcgctgt
      301 tcgccctgtt cagcacctgg tccggcgttc ctgtagaagg ttcgctggtg aacgtacgta
      361 ttatcgcggt gatgtcaggc gggattctgt ttggcccgtg ggtgggcatc attaccggcg
      421 tgattgcggg tattcaccgt tatttaattg atatcggtgg cgtgacggcg atcccctgct
      481 ttatcaccag cattctggcg ggttgtatat cgggctggat caacctgaaa atccccaaag
      541 cacagcgctg gcgcgtcggt attctcggcg gcatgttgtg tgaaacgttg accatgattc
      601 tggtgattgt ctgggcacca actaccgcat tggggatcga tatcgtctct aaaatcggga
      661 ttccaatgat cctcggcagc gtctgtatcg gctttattgt gttgctggta caaagtgttg
      721 agggcgaaaa agaggccagc gccgcgcggc aggccaagct ggcgctggat atcgccaaca
      781 aaacgctgcc gctgtttcgc catgtcaata gcgagtcatt acgcaaggtc tgcgaaatta
      841 tccgcgatga cattcacgct gacgcggtgg caatcaccaa taccgatcat gtgctggcct
      901 acgttggcgt gggtgaacat aactatcaga atggcgatga cttcattagc ccgactaccc
      961 gccaggcgat gaattatgga aaaatcatca ttaaaaacaa tgatgaagcc caccgcacac
     1021 cagagattca ttccatgctg gtgatcccat tgtgggagaa aggcgttgtg accggaacgc
     1081 tgaaaattta ctactgccac gcgcatcaga taacctcgtc attacaggaa atggcggtcg
     1141 gtctgtcaca aatcatctcc acgcaactgg aagtttcgcg cgctgagcag ctgcgtgaaa
     1201 tggcaaataa ggcagagctt cgcgccctgc aaagcaaaat taatccccat ttcctgttta
     1261 acgctttgaa cgctatttca tcgtcaatcc gtctgaatcc ggataccgct cgccagctga
     1321 tctttaatct gtcgcgttat ctgcgctata acattgaatt aaaagacgat gagcaaatcg
     1381 atatcaaaaa agagctgtat caaattaaag actatattgc cattgagcag gcccgctttg
     1441 gcgacaagct gacggttatc tatgatattg atgaagaggt gaattgctgc attcccagcc
     1501 tgctgatcca gccgttggtg gaaaacgcca ttgtccacgg tatccagcct tgcaaaggta
     1561 aaggcgttgt caccatcagc gttgcagagt gcggtaatcg ggtacgcatt gcggtgcggg
     1621 ataccgggca cggcatcgat ccaaaggtga ttgagcgggt cgaagcgaac gaaatgccgg
     1681 gcaataaaat tggcctgctg aatgtccatc atcgcgtgaa gttattgtat ggcgaggggc
     1741 tgcatatccg ccgcctggaa ccaggaacgg aaattgcgtt ttacattcct aaccaacgca
     1801 cccctgtcgc ctcacaggct acgttattgc tttgagccgg agtgatattg tgaaagtcat
     1861 cattgttgaa gacgaattcc tggcacaaca ggaactgagc tggctaatta aagagcacag
     1921 ccagatggag attgtcggca cctttgacga cggtctggac gtgttgaagt ttttgcagca
     1981 taaccgcgtc gacgccattt ttctggatat caatattccg tcgctggatg gtgtgttact
     2041 ggcacaaaac atcagccagt tcgcccataa accgtttatt gtgtttatca ccgcgtggaa
     2101 agaacatgcg gtagaagcgt ttgaactgga ggcgtttgac tacattctca aaccgtatca
     2161 ggagtcacgc attaccggga tgctgcaaaa actggaagca gcctggcagc aacagcagac
     2221 cagcagtacg cctgccgcga cggtaacgcg tgagaatgac accattaatc tggtgaaaga
     2281 tgagcgaatc atcgtcacgc caattaacga tatctattac gccgaagcgc acgagaaaat
     2341 gacctttgtc tatacgcggc gtgaatccta cgtaatgccg atgaacatta ccgaattttg
     2401 cagcaaactg ccgccatcgc attttttccg ctgccatcgc tcattttgtg tcaatctgaa
     2461 caaaatccgc gagatcgaac cgtggtttaa taacacctat attctgcggc tgaaagatct
     2521 ggattttgaa gtgccggtca gccgcagcaa agttaaagaa ttccgccagt taatgcatct
     2581 ttaatcggga actttcatga aagcaccagg cttgcccgcc gatcaacaat ttttcgccga
     2641 tctgttcagc ggcctggtgc ttaacccgca actactgggg cgcgtctggt ttgccagcca
     2701 gcctgcctcg ttaccggtgg gcagtttatg tattgatttt ccccgtctgg atatcgtgct
     2761 gcgcggcgaa tacggcaatc tgctggaagc aaagcagcaa cgtatggttg aaggagaaat
     2821 gctgtttatt ccggcgcgcg cggctaattt accgatcaac aacaaaccgg tgatgctgtt
     2881 aagcctggtg ttcgcaccga cctggcttgg gttatcgttt tacgatagcc gcaccacgtc
     2941 gttgttgcat ccggttcgcc agatccaact tcccagcctg caacgcggcg aaggtgaagc
     3001 aatgcttact gccctcaccc atcttagccg ttcgccgctg gagcagaata tcattcagcc
     3061 gctggtgtta agtttgctgc atctttgccg taacgtggtg aatatgccgc cgggcaattc
     3121 gcagccgcgc ggcgattttc tctatcacag catttgtaac tgggttcagg ataattatgc
     3181 ccagccgctc acccgtgaga gcgtggcgca gttttttaat atcacgccaa atcatctgtc
     3241 aaaactgttt gctcagcatg gaacaatgcg ttttatcgag tatgttcgct gggtacgaat
     3301 ggcaaaagcg aggatgattt tgcaaaaata tcatctgtcg attcatgaag tggcgcagcg
     3361 ttgcgggttt ccggatagcg actatttttg tcgcgttttc cggcgtcagt ttggtctgac
     3421 gccgggagag tacagcgccc gttttcaggg ctaacgtcag aaggttaatt ctgtttccag
     3481 cagcgtcagg atgctttgtg catcttgcgc ggcaaacaac gactggcgga agtttttatt
     3541 caccagttta cgcgccagct gcgagaagac tttcacatga ttcatccctt cgttagcgcc
     3601 cagtgtcagc atgatcacca gttcgacttc gcccatttct gactgccagt caaccggttt
     3661 caccagtcgg gcaatgctga tactggaatg acggatccac tgagatttgg tgtgcgggat
     3721 cgctacgcca aaaccaacgc cggtggtaac aatctcttcc cgctgccaga catcctcttc
     3781 cagctcgaac ggatgttcag tgcgcccgtt aacgccgagg ttaccgcaca ggaactggat
     3841 cgcctgctct ttattgctaa aagcctgatc aacaaagata ttctccagcg ccagcagagg
     3901 gcgaacgtct tcttccggcg taaacgcggt gagtaacgct tcaatctcct gcgcgctgcg
     3961 gcattcacat gcctgacgcg ccagttcccg acacgcctcg ctatccagtt gacgaagctg
     4021 gcttttcacc gccggaatgc gcgggctact catactcagc tcgtccaggc ccagcccaag
     4081 cagtagcggc agataacggc tttcaccgcc cagttcaccg caaataccta cccatttacc
     4141 ccgctgatgc gcagtggtaa caatctgctg caacatgcgc aggaacgatg gcgtaatcgg
     4201 gttatacagc ggcgatacgc gcgggttatt acgatcgacc gcatacagat actgggtcat
     4261 atcgttggag ccgatactga agaaatcgac ctcatcgcag aagtgatcga tgatgtagca
     4321 caccgacggc acttccacca tgatacccag cgtaatcgtc tctgcatgac gcaggccatc
     4381 gcgcttaagc tcaacgatcg ctttttggat ctcgcccttc acccataaga tctgatcgag
     4441 gctgtgaacc attgggatca tcaactgggc gttgccgaaa ctggcggcgc gcaaaatggc
     4501 ccgcagttga gtgcggaaca ggccagcaaa ttccgggtaa attcgtaccg cacgatagcc
     4561 gaggaacggg ttctcttcct ggggaatgtt gaggtaagga atgcttttat cgccgccgat
     4621 gtccattgtg cggaagataa tcggcttgtc gcccgcggcc agtagcacct gctggtaggc
     4681 ttcaaattgc tcctgctcgt ccggcgcact gtcgcgatcc atatagagca tttcggtacg
     4741 aaacagcccg acgccttccg cgccgttggc aaacgcgcca ggcgcttcca gcgcggtgcc
     4801 aatattcgcc gcgatatcaa tgcgcttgtt atcacgggaa taggccagct gcgcggcggc
     4861 ctgcgcctgc tgctgctgac gtttatccgc cagcatctgc gcaacctgat aataaccgct
     4921 caccgcgtca ttcgggttaa tcgccaacac gccgcactgg gcgtcaagca ccgccggttg
     4981 cccggcatag cgcgcaatgg catccagcgg caggccgctc agtaccggga tcgccgaggc
     5041 gcgggccaga atcagggtat gcgaggtgcg cccggttttc tccagaatca tgcccgcaag
     5101 attttttaaa ttgaggctca aaaactggct aggggttaaa tcctctgcca ccagaatggt
     5161 cggtttttcc agcaccagat tgttgcgcgg ttttagttcc ggccaggtga tatgcagtaa
     5221 ctgttcgctg atatcgcgaa tgtcgctaac ccgctcgcgc agataatcac tggcagaagc
     5281 agaaagtttg gcgcaaacct gctccatatt gctgatgatc gccgccccca gcccctgatg
     5341 ctgttctgcc atcaggtgac ggatattgcc tgcaaattca tcatcctgaa tcagcgacaa
     5401 atgggcgctg aggatagttt tgctttcgcc gtcacgctca cgcaattgct ggttcagttg
     5461 ctcggcaagc gttgccaggc tgtgctccag ccgggtggaa tcttgcgcac tggcggggat
     5521 cgcccggtaa ctgtcgaggc tgtcgctctg taacagggtc agcgtaccca cgccgacgcc
     5581 gcttgccagc acattgccgt acagtaaatc aggattcaga cggcttaatg aacgcggcag
     5641 cggatgcgcc gtcagttctg cctgcgttgg ctggacgctg tcgctgtcga taaagcgcac
     5701 ctggatgtac tcttccagca cgcgccgcgc ctgctcttca tcgctgccgc taatgttcag
     5761 gctgcaaccg tcattaaata gggtgccggt gccaatcagc gccagcgagc ttttggcatc
     5821 tgcctttgcg ttctggcgat ggttaataaa agtgatttcg ctttgccact ggctgcactg
     5881 ttctttaagt tcccacgccg gacgagcatg cagaccgttt ggcaaaggac agagaaattg
     5941 aatcgttaac ataaccgctc cgggttaatc aggagatcat ctgaaatccg tcagttgaac
     6001 aaccgtctca cgcgtaagac gttgaataag ggcagataaa agctgctgca tctgcaaaat
     6061 gtcacggcaa tcggcaatcg atgcggcgca atgtccgtgg cgggtggcag gccccatcac
     6121 cactgtgggt acgccagtgc cggttaagtg caccgcccca ccgtccgtgc caccgttgct
     6181 gaacatatcc gcctgcaacg gcacgccaat ttctgccgcc acggtttcaa tccacgcggt
     6241 aagttttggc ggcgcaatca gcgatttatc gcttaacacc agcatcggcc cgttaccaat
     6301 ctgacgatgg ttagccgcgc cataatcaaa gtttttcgcc cagcaggcgg tatcaagcac
     6361 aatggcgacg tccggcgaca ccgcgcgggt ggcagtttgc ccgccgcgta atcccacctc
     6421 ttcgctggaa cttgccacca gccacacctc cgcaggcagt tcggcgtcgt gcagctcacg
     6481 tagtaacgtc accagcagat agcaaccgag gcgatcatca aaggctttcc ccatcacccg
     6541 ctggtgaggg agaacctgaa aagttgtatc aaaggtgacg cgatcgcctg gacgaattcc
     6601 cgcctgcatc acttcgtcat acgagcgcgc accaatgtcc acgcgtatgg tgctgacgtc
     6661 attcccctgc cggtcgccgt caagcaggcc tggaattttg cactcttcac gggtagtgat
     6721 acgcaccgac tgaagctggc gggcagccat gcgtacattg ccaaccggca acacatcgat
     6781 cgccccttcg cgggagatgc tgcgcaccat aaatcccact tcgtccatat gcgcacagat
     6841 catcaccttc ggacctgtcg actcattgag tcggatcagc accgatccca gcccatcaaa
     6901 tcgcacttct ttttgcaggc gatccgcttc ttccagcagg atctgccgta cttcctgttc
     6961 cgaggaggcg atcgcatctg cctcgctcaa cgcttttaat aacgataaat ccattatgcc
     7021 tctcccgtga gcaacactgt tttcggcatg gcgtagagca cttctacgcc ttgcggggtg
     7081 accagcacaa catcttcgat gcgcacgccc ccttgccctg gcaaataaat ccccggctcc
     7141 acggtcagta acatgcctgg ctgtagcgtc gtggtgtccc gcggtgaaaa acgcggatcc
     7201 tcatggactt caataccgat agcatgaccg gtgttatgac cgaaatagtc gccataaccg
     7261 gcttctgtaa tcacccggcg cgcggcgtcg tcaacctgct ggcagcgtac gccggggcga
     7321 atcgcggaga ttgctgcgag ctgtgcctgc aggacaatct gatagacgtt aaacagcggg
     7381 tgggattcgg cacttacccc ttcgccattc accagcaagg tgcgcgtcat atcagagcag
     7441 tagccctgat acagcgcacc gaaatcgaga gtgacaaact cgcccgctgc aacaatcttg
     7501 tcgctggctt tgccgtgcgg cagcgcccca cgccagccgc tggcgacaat ggtatcgaaa
     7561 gaggcttttt ctgcgccctg ctggcgcata aaccactcca gttcagcggc tatctcgcgc
     7621 tcgctcatcc ccgcctgaat aaagcggcga atatgctctg caccgcgatc ggcaatccca
     7681 caggcgagac ggattttctc cacctcctct ggcgttttga gttgccgcag cacatccggc
     7741 gtggcgctaa ccagtttcgc attgagttca gactgccagc ggtgcgcggt ttcccaactc
     7801 acctgctggc cttcaaaacc gagcgtttgc aactgctcat cggcaaagat ttgcctgacg
     7861 atggtggcaa gcgtgtgcat cgcgtcgagc aaatgcagct ggtaaccttg cgtgcgggct
     7921 tctacatccg cgtaatagcg cgagtccacc agaatgtgcg cactttcacg gcaaatcacc
     7981 acatagcctg atccggtgga gatccccaga tgcggctgtt tgttctgccg tgaggagaga
     8041 agcaccgcat ccagttgttg cgccttaagc cagtcgcgca gcgaagcgag taatgtcatc
     8101 aggattgtcc ttcttacagg ctatcgatta acaatttgcc tttacggaac atcatcagac
     8161 gcaggaacac caccatcagc gcagttatga ctgcccccag cgcgatcccc gccatgtaga
     8221 cgccgaggtt agtgaccagc ggccatgccc agatagcgga ctccgggaac cattgcactg
     8281 cgcccagcca gacagcggcg gttgagccga caatcgcgcc gaccatatag gacggaatgg
     8341 cggtgatggg gctttccagc gcaaacggaa tcgcaccctc actgatcccc ataaacgcca
     8401 ggaacatggc ggttttaccc tgcggataaa gctgagcact gaacagacgt ttgccggtta
     8461 aacggcggtc gataatggtc gccagaccca gaccaatcgg cggaataaca atagcgatgg
     8521 aacgcgcggt aaccggtaat acgtggtcag tggtaaagct gaaggcaacg aaaccagctg
     8581 ctttgttgat cgggccacca aggtcgattg ctgtcgccgc ggcgatcccc atcgcgtaca
     8641 ttagcgcacc cttctcacct gcggcagtca gcacggtacg gataccgccg ttgatccagc
     8701 caccaaaggg ggtgatgacg tagtacatcg ccagcatgac aaaaatggcg gaaagaatcg
     8761 gtagcaaaaa tgtggttttg aacgctaaca ggaaatccgg taactggatt ttctggttca
     8821 tccacttcac aaggtaacca gcaacgatag aaataatcag ggcaccaatg aaggtggacg
     8881 gcaccggcga agaggtcgcc cattgcatcg tgctgggatc gaagttcagc agttgcgtcg
     8941 gctgggtaga catcaaccca ccgataaaac cagccgggaa cgccagtttg ccaccgatag
     9001 agttcgccac aaatgcagca aacatcggaa tggcaaaacc aaacaacacg ccgccgaagg
     9061 actgcgacaa ccaggcaaat ttcagtaagg agaggtcgaa accggagaat ttcccgctat
     9121 taagagcatc catgatgccg atatcagcag gaattttaag ccagctataa gcaatcagct
     9181 ggctgaaagc gaggatcaca ccgcccataa ttaaggtcgg caccatgcgt gaaatccctg
     9241 acatcacatg ctgcgggagt tcaccccaga agctggtttt agaggcctgc ggattcttaa
     9301 ctgccgctgc cgcaccggat gcgccaggca caacggttgc actgcgtttt ttaatggcca
     9361 taacaattat ccctgtcgaa tattactgtt gttcagaggc aatcatctct tcgatttctt
     9421 tgatgatgcc cgcagcgttt ttaattgcgt cctgcaaagt gatttcataa acgtcgcgtg
     9481 attcgaaacg ttcgttatct tccggggtaa ccgccacgga gtggatgatg atggtcgctt
     9541 cggcgatatc ctgcgcggtc aggcgattct ggatgccgtc cgcgccctga gtttcaattt
     9601 tcacttcata gcctgcttcc accgccgctt cttccagcgc ctgggctgcc ataaaggtgt
     9661 gagccagacc catcgggcag gcacataaag caatcagttt cttactcatc gttaattcct
     9721 cgttcagtga tagttgacga gtgaaattat gaacgtgcaa aacaaatcgc cgttaccaga
     9781 caaataatgc atttactgga gaattaatcc cgctgtacgg aagtgtgacg caggtcggcc
     9841 ttgtgagggg acacaaagga aaattgaatt aactgtatga tttaaaagat tatcgggaga
     9901 gttacctccc gatataacag gaaggattta gagaatgtga cctaaggtct ggcgtaaatg
     9961 tgcaccggaa ccaagaaggc ccggattgtc atggacgatg agatacaccg gaatatcatg
    10021 gacatattct ttaaagcgcc ctttatcttc aaatgcggca cggaaaccag aggctttgaa
    10081 gaactcaagg aagcgcggca cgataccacc cgcaataaac acgccgccaa atgtcccgag
    10141 attgagcgcc agattgccgc caaaacggcc cataatgacg caaaacagcg acaacgcgcg
    10201 gcggcaatcg gtgcagctgt cagccagcgc gcgttcggta atatcttttg gcttgagatt
    10261 ttctggcagg cggttgtcgg ctttaacaat tgcgcgatac aaattcacca gcccagggcc
    10321 agaaagcacg cgctctgccg aaacatgacc aatttctgca cgcagtattt cgaggataat
    10381 gccctcttct tcactattcg gcgcaaaatc aacgtgaccg ccttcgcctg gcaagcttac
    10441 ccaacgctta tcgacatgga ccagatgcgc aacccctagc cccgttccgg caccgtaaac
    10501 cgcaataggc ttaccttcta ccggttctgc gccaccaaac tgaatcagat gctctttttt
    10561 cagcatcggg atcgccatcg atacagcggt aaaatcgttg ataatttcca gatggctaaa
    10621 accgagattc tttttcattt cggcaattga gaacgcccag gtatggttgg tcattgccac
    10681 ccagtcaccg gtaattgggc aagcgatggc aatacagccg tcttgcacct cgatcttatg
    10741 ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc ctgaataggt
    10801 cttagcctgc gagatttcac cactggcaat atcacacaga gcaagacgtg cgttggtgcc
    10861 gcccacatca ccgactaatg catactttgt cattcttcaa ctgctccgct aaagtcaaaa
    10921 taattctttc tcacactgta aatacctggg ggcataacaa caacgccgga aaggcaggct
    10981 ccctgtaaat atcgatctgg gtcacacaat tactttatcg tttcagcacc aattgcagcg
    11041 atgccttttt gcaagctggg caaactaagt atctgacccc gcataaggaa tagaacatgc
    11101 tccatccgcg agccagaacc atgttgttat tatcgctccc cgccgtggca attgggattg
    11161 cgtccagtct tattctgatt gtggtgatga aaatcgcctc ggtattacag aatttgctct
    11221 ggcaacgact gccgggaact ctggggatag cccaggattc acctctctgg atcatcggtg
    11281 tattaacgct aacgggtatt gcagtggggt tggttatccg tttcagccag ggtcatgccg
    11341 gaccagaccc cgcctgtgaa ccgctgatcg gcgcaccagt tccgccctct gcgctaccag
    11401 gacttatcgt agcattaatt cttggtcttg ctggtggcgt cagcctgggg ccagaacatc
    11461 cgatcatgac cgtcaatatc gcccttgcgg ttgccattgg cgctcgtctg ttaccgcgcg
    11521 tcaaccgaat ggagtggact attttagcct ctgccggaac catcggcgcg ctgtttggca
    11581 cacctgttgc ggcggcgttg atattttcgc aaaccttaaa tggcagtagt gaagttccgc
    11641 tatgggatcg tctttttgcg ccgttaatgg cggcagcagc tggtgcacta actaccggat
    11701 tatttttcca tcctcatttt tcactgccca ttgcgcatta cggacagatg caaatgaccg
    11761 atattctcag tggtgcaatt gtcgcggcga ttgccatcgc agcagggatg gtcgccgtat
    11821 ggtgcttacc acgattgcac gcgatgatgc atcaaatgaa aaatccggtg ctcgtgctgg
    11881 gtattggcgg atttattctc ggtattctgg gggttatggg tggaccagtt tcgctgttta
    11941 aagggctgga tgagatgcag cagatggtgg caaatcaggc tttcagcacc agcgattact
    12001 ttttgctggc ggtgattaaa cttgccgccc tggtcgttgc tgccgccagt ggctttcgcg
    12061 gtgggcgaat cttcccggca gtgtttgtcg gcgtggcatt agggttgatg ctgcatgagc
    12121 acgtccccgc cgttccagcg gcaataaccg tttcctgcgc tattctcggc atcgtgctgg
    12181 tggtaacacg cgatggctgg ttaagtcttt ttatggcggc agtcgttgta cccaatacca
    12241 cattgctacc gctgctctgt atcgtcatgc ttccggcatg gctgttatta gcaggtaagc
    12301 cgatgatgat ggtcaatcgt ccgaagcaac agccccccca cgataacgtt tagcaaaaaa
    12361 tgctctcctt tatgatttaa gagttatggc gatgatacgg ggctttacct ccccgtaata
    12421 ttgcgttaac aggccgctga cggcttcgat cgtgaaggag aataacaatg ttcaggtcac
    12481 tgtttctggc ggccgccctg atggcattta ccccgcttgc ggcaaacgca ggtgaaatca
    12541 ccctactgcc atcaatcaaa ttacaaattg gcgatcgcga tcattacggt aattactggg
    12601 acggtggtca ctggcgcgac cgtgactact ggcatcgcaa ttatgaatgg cgtaaaaacc
    12661 gctggtggcg tcatgataat ggctaccacc gtggctggga taagcgtaaa gcgtatgagc
    12721 gtggctatcg tgaaggctgg cgcgatcgtg acgatcatcg cggaaaaggc cgcggacatg
    12781 gacaccgcca ttaaacacat tcgccaatgg agcacaatgc ctgatgcgac gctagtgcgt
    12841 cttatcaggc ctacaaaccg gctctttaac gcgtagggcg gataaggcgt ttacgccgca
    12901 tccgccaata gtgccagatg cgacgctcat tcaactacaa tcccagcgcc gtccccacca
    12961 acaaccagat attcagcgcc acgaccagca ctacaatcac ccagcctgtc tgttttacgc
    13021 gtttgctgtt caccagatcg cccatcaact tgctgtcact ggtgaaaatc agcagtggaa
    13081 ccagcgccag agcgatacca aaacttaaca gcacctgact cataaccaga atccgtgtcg
    13141 gatctaatcc catcagaatg acaataaatg acggcaacat ggtaacggtg cgacgcaccc
    13201 acagcgggat atggaagcga atgaagccct gcatcaccac ctgccccgcc agtgtcccca
    13261 ccaccgttga tgacagtccg gcagcaacca ggcttaatcc aaagaccgtt gccgcagcat
    13321 ggcttaacaa cggttgcagc gtcaggtaag cctcatcaag atcggcaacg ccagtatgac
    13381 cggaaaagtg gaacgccgcc gcagccgtag ccatcatcgc cagattgaca aaaccggcaa
    13441 tagtcatagc gatagccaca tcccatttgg tggcagaata acgttgttga cgcgaaccac
    13501 catgtaaatg ctgagtcagc gaagagtgta aataaatcac gtgtggcata atcgtcgccc
    13561 ccaacacacc tgccgccagg aagactgcct ccgaggtggg taaactcggg attaccattc
    13621 ctttacccag ttgcgccagg ttaggctggg agaaaatcaa ctcgacaatg taagccgcag
    13681 caacaaacag cagtaacccg ccaatcactt tctccagcgg tttttgccct cgacgttgca
    13741 gcattaaaat caggaaagtc gcgatccccg tcagcaccgc gccctgcaac agcgaaacac
    13801 ccagaatgag tttaaaaccg atcgccgcac caataaattc cgccaggtcg gttgccatcg
    13861 caatgatttc tgcctgaacc caatagaacc acactacggg acgcggatag tgatcgcgaa
    13921 tctgctccgc caggttttta ccggtggcaa tccccagttt ggcagacagt atctgaatca
    13981 gcatcgccat caggttggcc caaacgacaa cccacagtag ctgatagccg aagctggcac
    14041 ccgcctgaat attggtcgca aagttaccag gatcgatata accaatcgcc gcaatgaacg
    14101 caggtcccat taatgcgagc ctcatcttgc gcgccgcccg tccgctgcta ctctcaacgc
    14161 gatagttagt catcttgtgc ctctaaaaca tagcctttgc tatgtttcat gctatgtcaa
    14221 acgagaatga ttatcaaatt catttaagtg gattgtgatg atttctttga tagaccagaa
    14281 ttatgacgac gaaaagattg atgacttgaa tgttgtgatt atgtttaatg ttagcacatt
    14341 tacataactt tcagcttcca tacacaacat agcagaaatg tatgacagct cactattttt
    14401 gaagcttgtc acaggacgtc attatagtgt gtgtcagatc tcgttttcct taaccatgtt
    14461 acatagaatg tgcacggaaa tttaacctgc ctcatatttg gagcaaatat ggaccgcgtc
    14521 cttcattttg tactggcact tgccgttgtt gcgattctcg cactgctggt aagcagcgac
    14581 cgcaaaaaaa ttcgtatccg ttatgttatt caactgcttg ttatcgaagt gttactggcg
    14641 tggttcttcc tgaactccga cgttggtttg ggcttcgtga aaggcttctc cgaaatgttt
    14701 gagaaactgc tcggatttgc caacgaaggg actaacttcg tctttggtag catgaatgat
    14761 caaggcctgg cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat ctctgcgctg
    14821 atcggtattc tccagcacat tcgcgtgttg ccggtgatta tccgcgcaat tggtttcctg
    14881 ctctccaaag tcaacggtat gggcaaactg gaatccttta acgccgtcag ctccctgatt
    14941 ctgggtcagt ctgaaaactt tattgcctat aaagatatcc ttggcaaaat ctcccgtaac
    15001 cgtatgtaca ccatggcagc aacggcgatg tccaccgtgt cgatgtccat cgttggtgca
    15061 tacatgacca tgctggaacc gaaatacgtc gttgcagcgc tggtactgaa catgttcagc
    15121 acctttatcg tgctgtcgct gatcaaccct taccgtgttg atgccagtga agaaaacatt
    15181 cagatgtcca acctgcacga aggtcagagc ttcttcgaaa tgctgggtga atacattctg
    15241 gcaggtttca aagttgccat tatcgttgcc gcaatgctga tcggctttat cgccctgatc
    15301 gccgcgctga acgcactgtt tgctaccgtg actggctggt ttggctacag catctccttc
    15361 cagggcatcc tgggctacat cttctatccg attgcatggg tgatgggtgt tccttccagt
    15421 gaagcactgc aagtgggcag tatcatggcg accaaactgg tttccaacga gttcgttgcg
    15481 atgatggatc tgcagaaaat tgcttccacg ctctctccgc gtgctgaagg catcatctct
    15541 gtgttcctgg tttccttcgc taacttctca tcaatcggga ttatcgcagg tgcagttaaa
    15601 ggcctgaatg aagagcaagg taacgtggtt tctcgcttcg gtctgaaact ggtttacggc
    15661 tctaccctgg tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta agaccataca
    15721 taaaaaagcc ggggataatt ccccggtttt ttacgcctgt taatcagcta atggctgcgg
    15781 gcgaccaatc aaataccctt gcagatattg caccccgagc ttatgcaata gcgcctgctg
    15841 ctgttgcgtc tcgacaaact ccgcgaccac actcaatgac ttcgctttcg ccagatcggt
    15901 aattgatctc acaatcatcg catccagcgt gttcgtgacg atatctttca caaagacgcc
    15961 atcaattttg atgatatcag cctgcaaacg ctttaaccgt tcgtagttgg catatccggt
    16021 gccaaagtca tcaatcgcaa tccggaaacc aaacttatgc agctgctcga tgttgtacat
    16081 gctgctttct gcgttagaaa acgcctgctc ctcggtgatc tcaagaatga ccgcctgcgg
    16141 ggagatgtga taacgcttaa acagacgaat aatccgcccg gcaatattct tttgcagcag
    16201 cgtgagcggc attaaattga ctgaaaagcg cgggcctttt ttgtcgcaag gatgtgttgc
    16261 cagccacttc aacagggatt ccagcacttg caaatcaaaa cgcgcgctaa ggttaaactg
    16321 agcaataagg ggcagaaact tatccggggt cataatgccg ccgtcatatt tcagtcgcgc
    16381 gaggatctca tcataacctt caccctcttt gttgcgaatt ggctgggcgt agagcagcaa
    16441 atcaccctga tctaacgccg tacgaatggt attcagcaat agcacctgtt tagtggtctg
    16501 cccggaaacc atctcctctc tgctatccag cgccagcaca tggtgatgtg cgcaggattg
    16561 ctccgccagc cagcttaact gccccaacaa gggttgcagg gtttcctgat ttccatcaaa
    16621 acgcccccag gcagcaccat agcccatatc cagcccggta ttgttccagt gaatttgccg
    16681 actattcagg atgttaacca tatgctggag tcgcccttcc gtttccggtc cactcagcac
    16741 taacagcagt tcactacccg gcaattgata caacttttcg ttttcctgca tcaacggcag
    16801 cagcgtacgg taaattgatc ggatacagtg aacgcgcatc attaagccgt aatgacgact
    16861 cataaactca agattatcaa tgcgcaggca gcaaaaactc ttgcccgcct cttgctccgg
    16921 cgcttgttct aacgcacgaa agttgggtag gagcgttaac ggatcggtca gcgcctgcaa
    16981 atgccagcgg cgattaagcc attcactgcg atgataaatg cgcaccatat agagcaggca
    17041 aacgctaaag gaaatcagca ccgcgagaat aaatgccagc gaatattcgg ttagtacccc
    17101 ttgcaaaaag ttctggttgt aattcagcag gcaaagcgtt gaaacagccc aggtgagatt
    17161 taaaaacgga tagcgaagct taccgacacc gagggtaaag atgataaaga aaacaggtac
    17221 cagataacca gcaataaagt cattttcata cggtgtgcac atcagaagta gcagcacgct
    17281 aagagctgcc agccagctta aggtaaatgc gcgtttctct ttgctcaacg acggagcgat
    17341 atccctacgc cacaatatct gtgcaaagtg gggacttaca atcatgcggg cgagatagta
    17401 aaaaagcatg ttgtaaatca gcactgcggt gaaaaggctt agcaaatcaa cgaccgtgaa
    17461 aatggcatcc gcatcgccga aaaaggtaga tatctttagc ggaaaatcaa agaaatttcc
    17521 cacaagatac atgctgcatt tgatgccaat cggcgccacc aaaccaagcc agaaaagacg
    17581 ttgccagaca tatcggctgg tcaatccata acgccaacgc gtccccagct gccagcgtaa
    17641 tattgcgcag gcacacaaca ccacaaacgt ctggctgccg agcatgaccg cggtctgtaa
    17701 aaaagagaga tgaaagttcc acagattggt gcaaaacatc ccgcataaaa cggggacgac
    17761 accgcgccag ccaaagataa acaagattga cagcattacg cacagtggca tccacgccag
    17821 aaagatataa ctggagtgga taatggcaag tggcgaaata aaacgggata gctgaataag
    17881 taccacggtg agcgtaaacg ctagtgtgaa tatcttgata ttttttatca ggttatgctc
    17941 cacaaacatg agtacactac tctcactgat cggggttttc aggcacacaa ataataagtt
    18001 tattcgcatc gcgaagcaaa caggggccag cctgttgccg atttttcatc caatgatgct
    18061 ttcaccaggc ggcgctaacc cggacgtaga ctgtattaca aaagcggcaa aaagcagaga
    18121 caaaaaaccc ccgctttaca gcgagggttt gaaatttggt ggagctaagc gggatcgaac
    18181 cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg agctatagcc ccacgatgcg
    18241 tttacgtacc aaatttgctg ggtgcaaaat ttggtggagc taagcgggat cgaaccgctg
    18301 acctcttgca tgccatgcaa gcgctctccc agctgagcta tagccccatc acgtaaagct
    18361 tgtcgagttg acgggcggca tcatatgaat tccgcccgaa tgtgtcaacg gcaaattgcc
    18421 acgtgtagtt tcaatcgctg aaaaatcagg caaatgaaca attttggaaa accgctcgca
    18481 ttcagtagtt attcatgtca cggtttcctg taaagtggtg ttataaaatg aactactaat
    18541 agacccacat acattcaggg aattgttatg ttcaaggaga ggatgacgcc agatgaactt
    18601 gccagactga ccggctatag ccgccagacc attaataaat gggtacgcaa ggaaggctgg
    18661 acaacgtcac caaaaccagg cgtccagggt ggcaaagctc gactggttca cgtcaatgaa
    18721 caggttcgtg aatatattcg caatgctgaa cgtccagaag gtcagggaga agcgccagcc
    18781 ctttctggtg atgcgccgct tgaagtgttg ctggtgacac tggcgaaaga gatgacgcca
    18841 gttgaacaaa aacagtttac atccttgctt ctgcgggaag ggattatcgg attgttacaa
    18901 cgcttaggga ttcgcgatag caaataatat gaaaagatta cgcaataaaa tgaccaccga
    18961 agaactggct gaatgcctcg gtgtggctaa acaaactgtt aaccgttgga tcagagaaaa
    19021 aggctggaaa acggaaaaat ttcccggcgt gaaaggcggt cgtgccagac ttattctggt
    19081 cgatacgcaa gtttgcgagt ttattcagaa cacgccagcc ttccataaca caccgatgct
    19141 tctggaagcc gaagaacccc ttgccgaata cgcaccgggt attcgcactc ctgcttatcg
    19201 gcaaatcatc agcgcaatag acaacatgac tcacagtgag caggaaaaag ttgcgcaatt
    19261 tttgtcacgc gaaggaattc gtaatttcct tgctcgtctc gacatagatg aatcagaata
    19321 aataaaaaac ggcaggggta tctctcctgc cgtttatctt ttatgcgcta attattgctg
    19381 attttcgcgt tcagcaataa aagccagcgc tttgttgata cgctcgatac tgcgggtctt
    19441 accgattgcg tgaacggtaa catccagcgc cggagactgc cccgcaccgg ttacggcgac
    19501 acgcagcggc ataccaactt tacccatacc cacttccagc tcatccgccg tcgcctgaat
    19561 agcgtgatga acgttttcag cggtccagtc agtaatcgca gtcagtttgt cacgaaccac
    19621 ttccagcggc tgacgcgcta ccggacgcag atgttttttc gcggcgtcgg catcgaactc
    19681 agcaaaatct tcgtagaaat aacggcagct ctgtgccatc tctttcagcg tcttgcagcg
    19741 ttcgcccagc agtttcacca gatcagccag ctgcgggccg ttacgggtat cgatattttc
    19801 ctgctcaatg tgccattgta agtgagtagc aacatactcc ggcggcagcg cgttaatgta
    19861 gtgatggttc agccacagca gcttgtcggt gttgaacgca ctggcagatt tgctgacggc
    19921 attcagagtg aagtatttga tcatctcttc acgagtgaag atttcctgat cgccgtggga
    19981 ccagcccaga cgcaccagat agttcagcag tgcttctggc aaataaccgt catcacgata
    20041 ctgcattacg ctgaccgccc cgtgacgttt ggacagtttt ttaccgtcat cgccgttgat
    20101 catagaaacg tgcgcgtaaa ccggtaccgg cgctttcagg gctttaagaa tgttgatctg
    20161 gcgcggcgtg ttgttgatat ggtcttcgcc acggataaca tgggtgattt ccatatccca
    20221 gtcatcaaca accacacaga agttataggt tggggaacca tcggtacggc gaatgatcag
    20281 atcatccagc tcctggttgc tgaactcaat cgggccacgg atctgatcgt caaaaacaac
    20341 agaaccttcc tgcgggttag caaaacgtac aacgcacggt tcatcatcag catgatgctc
    20401 atggctgtgg cggcagcgac cgtcataacg cggcttctca cctttcgcca tttgctcttc
    20461 gcgcagcgct tccaggcgct ctttagaaca atagcattta taagctgtgc cctcttccag
    20521 catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg gtctggtagt acggaccttc
    20581 atcccactcc aggctcagcc agttcatgcc atccataatg gcttcgatag cttccggcgt
    20641 ggaacgctca agatcggtgt cttcaatacg cagcacgaac tcaccgccgt ggttacgtgc
    20701 aaaaagccag gagtaaagag cagtacgcgc gccgccaacg tgcagatagc ctgttgggct
    20761 tggcgcgaag cgagttttga ttttcatgaa atggccttac gtttagaaag atgccgacaa
    20821 ccggcaaatc ctggaaaaat tgagtgggcg atattctatc actcccgcct gattcctcaa
    20881 tgtagttcgg gttgcaacct tacgcattat tgttttcgtt gacaaattgc gcaacacggt
    20941 ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa caatttcttt agaaaatgcg
    21001 ttgactcatt ttgaactctc cctataatgc gactccacac agcgggggtg attagctcag
    21061 ctgggagagc acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc
    21121 aactacttta tgtagtctcc gccgtgtagc aagaaattga gaagtgggtg attagctcag
    21181 ctgggagagc acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc
    21241 actttctcgc cagctagatt tcttgtaaca atgtgaagta ccgaagtggg tgattagctc
    21301 agctgggaga gcacctccct tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca
    21361 ccacttcggg tcgttagctc agttggtaga gcagttgact tttaatcaat tggtcgcagg
    21421 ttcgaatcct gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt ttttgctatc
    21481 tgcaatttgc gaaattgcct gatgcgcttc gcttatcaga ccaactatgt ccggcagttc
    21541 ccgtgcctct catctactgc gccaacgccg ccagcagttt aaccagcgcg ggcgtcacct
    21601 gctgcgtttc ataaacaata tataagtctg ccgggatgcg ttctttgagc ggacggaata
    21661 tgacaccggg ccagttcatc tgcgcgtagc tgtccgctat caatgtgata ccaataccca
    21721 tactgaccat ggcgagtacc gtttgcggtt cattaacttc gcgaataaca accggtgaaa
    21781 atcccacctg ctggcaaact cgctgcaaaa aatcccagtc agtgtacacg ggcggcattg
    21841 taacgaaata ctcgtcacgt agcgcttcca gcgggacggt ggaaaatgat gagagatgat
    21901 gctcttcagg catcgccacc agaaacgccg attcatgcaa ccgtaagctg gtaaaaccag
    21961 tcggtggttc tgtcgccatt cgccagatcc cggcatcaag ttcgcggcgt tccagcaagg
    22021 ccatttgcat cgcaggcatc ttttcgcgaa acagaacttc aacgttagga ttttctctaa
    22081 ggaatcgccg cataaccggg cgcatccgtc cccacattgc cgttcccaca accccgagtt
    22141 caatccgccc tgcttctccc cgacctattt gttcaacccg agccaataca ttatttgcat
    22201 tcaccagcaa tcgacgcgat tcttccatca agattttgcc cgcgtgtgtc agtacgacgc
    22261 tgcgcgaatg gcgaataaaa agctgcgtgc cgagttgatt ttccagctct ttaatatgaa
    22321 tgctgagcgg aggctgagac atatttaaac gcgctgctgc gcgaccaaaa tgcaactctt
    22381 ccgctacggc aagaaaataa cggagcaact taagatctgt tctgtatacg cgttc
//
LOCUS       DAZKGF010000077        22367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZKGF010000077 DAZKGF010000000
VERSION     DAZKGF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.077"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..573
                     /locus_tag="ACHOQV_002744"
     CDS             <1..573
                     /locus_tag="ACHOQV_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9631094.1"
                     /translation="MLRLRRAGQITGQHVPEIILLNSHDGSSSYQMLPGYFRAICTNG
                     LVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLILPPPARQALAQ
                     AALTYRYGNEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRI
                     HTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            912..1385
                     /locus_tag="ACHOQV_002745"
     CDS             912..1385
                     /locus_tag="ACHOQV_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9631095.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFSLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNVMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            1401..1877
                     /gene="yeeS"
                     /locus_tag="ACHOQV_002746"
     CDS             1401..1877
                     /gene="yeeS"
                     /locus_tag="ACHOQV_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP9631096.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            1946..2167
                     /gene="yeeT"
                     /locus_tag="ACHOQV_002747"
     CDS             1946..2167
                     /gene="yeeT"
                     /locus_tag="ACHOQV_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9631097.1"
                     /translation="MKIITRGEAMRIHQQHPASLLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            2330..2698
                     /locus_tag="ACHOQV_002748"
     CDS             2330..2698
                     /locus_tag="ACHOQV_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9631098.1"
                     /translation="MSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            2788..3165
                     /locus_tag="ACHOQV_002749"
     CDS             2788..3165
                     /locus_tag="ACHOQV_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9631099.1"
                     /translation="MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFS
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRFSTSPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKR"
     gene            3162..3650
                     /locus_tag="ACHOQV_002750"
     CDS             3162..3650
                     /locus_tag="ACHOQV_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9631100.1"
                     /translation="MKLSLTLEADSINVRALNMGRIVVDVDGVELAELINVVCDNGHS
                     LRVVDESDRASTDCTPPFAALTGIRCSTAHITAKDSAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            3670..3867
                     /locus_tag="ACHOQV_002751"
     CDS             3670..3867
                     /locus_tag="ACHOQV_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9631101.1"
                     /translation="MESLTTETALDILIAWLQNNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            3952..4818
                     /locus_tag="ACHOQV_002752"
     CDS             3952..4818
                     /locus_tag="ACHOQV_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9631102.1"
                     /translation="MSDITASRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMQSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHRSKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISGMQADRLADLERMLHLLSGKPIPDNR
                     GNITINLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            4890..5153
                     /locus_tag="ACHOQV_002753"
     CDS             4890..5153
                     /locus_tag="ACHOQV_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP9631103.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            5150..5476
                     /locus_tag="ACHOQV_002754"
     CDS             5150..5476
                     /locus_tag="ACHOQV_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9631104.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(5890..6426)
                     /gene="yghD"
                     /locus_tag="ACHOQV_002755"
     CDS             complement(5890..6426)
                     /gene="yghD"
                     /locus_tag="ACHOQV_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFP9631105.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(6428..7605)
                     /gene="gspL"
                     /locus_tag="ACHOQV_002756"
                     /pseudo
     CDS             complement(6428..7605)
                     /gene="gspL"
                     /locus_tag="ACHOQV_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
     gene            complement(7602..8579)
                     /gene="gspK"
                     /locus_tag="ACHOQV_002757"
     CDS             complement(7602..8579)
                     /gene="gspK"
                     /locus_tag="ACHOQV_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFP9631106.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVAQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(8576..9181)
                     /gene="gspJ"
                     /locus_tag="ACHOQV_002758"
     CDS             complement(8576..9181)
                     /gene="gspJ"
                     /locus_tag="ACHOQV_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFP9631107.1"
                     /translation="MRRTCAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(9178..9549)
                     /gene="gspI"
                     /locus_tag="ACHOQV_002759"
     CDS             complement(9178..9549)
                     /gene="gspI"
                     /locus_tag="ACHOQV_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFP9631108.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(9546..10109)
                     /gene="gspH"
                     /locus_tag="ACHOQV_002760"
     CDS             complement(9546..10109)
                     /gene="gspH"
                     /locus_tag="ACHOQV_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFP9631109.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQAF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYA
                     SAQNACWAVKLAHDGVLSLNQCDEGMP"
     gene            complement(10113..10568)
                     /gene="gspG"
                     /locus_tag="ACHOQV_002761"
     CDS             complement(10113..10568)
                     /gene="gspG"
                     /locus_tag="ACHOQV_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFP9631110.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(10585..11808)
                     /gene="gspF"
                     /locus_tag="ACHOQV_002762"
     CDS             complement(10585..11808)
                     /gene="gspF"
                     /locus_tag="ACHOQV_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFP9631111.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RLNASTGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(11808..13301)
                     /gene="gspE"
                     /locus_tag="ACHOQV_002763"
     CDS             complement(11808..13301)
                     /gene="gspE"
                     /locus_tag="ACHOQV_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFP9631112.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTVQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(13301..15361)
                     /gene="gspD"
                     /locus_tag="ACHOQV_002764"
     CDS             complement(13301..15361)
                     /gene="gspD"
                     /locus_tag="ACHOQV_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFP9631113.1"
                     /translation="MFWRDMTLSIWRKKTTGLKTKKRLLPLMLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDDQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(15391..16350)
                     /gene="gspC"
                     /locus_tag="ACHOQV_002765"
     CDS             complement(15391..16350)
                     /gene="gspC"
                     /locus_tag="ACHOQV_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFP9631114.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHILSQRQSLNTDKEHLRKMVRGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTVVSSSANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEERPPVAVTSKKAVSDEAKQAVAEPAASAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFREGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(16368..16778)
                     /gene="gspS2"
                     /locus_tag="ACHOQV_002766"
     CDS             complement(16368..16778)
                     /gene="gspS2"
                     /locus_tag="ACHOQV_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFP9631115.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISESGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(16844..17653)
                     /gene="pppA"
                     /locus_tag="ACHOQV_002767"
     CDS             complement(16844..17653)
                     /gene="pppA"
                     /locus_tag="ACHOQV_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFP9631116.1"
                     /translation="MFFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSTQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGALSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLKALF"
     gene            complement(17783..22338)
                     /gene="sslE"
                     /locus_tag="ACHOQV_002768"
                     /pseudo
     CDS             complement(17783..22338)
                     /gene="sslE"
                     /locus_tag="ACHOQV_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
BASE COUNT         5092 a         6302 c         5659 g         5314 t
ORIGIN      
        1 atgctgcgtc tgcggcgggc cggacagata accggtcagc atgtgcctga aattattctg
       61 ctcaactccc atgacggttc atccagctac cagatgttac ccggatattt tcgtgccatc
      121 tgcaccaatg gcctggtctg cggtcagtcg ctgggagaag tccgggtgcc acaccgggga
      181 aacgtggtgg acagggtgat tgaaggggct tacgaggtgg tgggcgtttt tgaccggata
      241 gaggagaagc gtgatgccat gcagtcgctg attctgccgc caccggcacg ccaggcgctg
      301 gcacaggcgg cactgactta ccgttatggt aatgagcatc agcccgtcac caccgctgat
      361 attctgacac cgcgacgccg ggaggattac ggtaaggacc tgtggagtgc ttatcagacc
      421 atacaggaga atatgctgaa aggcgggatt tccggccgca gtgcaaaagg aaaacgtatc
      481 cacacccgtg ccattcacag catcgacacc gatattaagc tcaaccgcgc attgtgggta
      541 atggcagaaa cgctgctgga gagcatgcgc tgataaaaca tatccctgaa ggcgtgattc
      601 aggtaccaga atcacccgac gccccggagg cgctgccggg gtgtcgttcc atttctgatg
      661 atgacctgtg cacctggtgt acccgtctgc tttaccgtcc cggagaactc agtctgtgca
      721 gactgagtac aggcaatggc atctggccat ccgtctgtga ccggaacggt tacgcgtacg
      781 gctgtccgga atttcagccg aatatcattc atccctgata ttgccgggtc tcacggtcag
      841 tgatgaacgt gatgttgtca gcccccggcc tttatttcct ctcatttatt cacattacaa
      901 ggatttcaca catgaaaaca ttgtctcaga acaccacctc ttcagcctgt gcaccggaga
      961 ctggcctgca acaactggtt gccaccatag tccctgatga acagcgcatc agcttctggc
     1021 cgcagcattt tagcctcatt ccacagtggg tgaccctgga gccccgtgtc ttcggctgga
     1081 tggaccgttt gtgcgaagac tactgcggtg gtatctggaa tctgtacacc ctgaacaacg
     1141 gcggcgcatt tatggcccct gaaccggatg acgatgatga cgaaacatgg gtgctgttca
     1201 atgtcatgaa cggtaaccgc gctgaaatga gtccggaagc cgccggcatt gcggcctgtc
     1261 tgatgacgta cagtcatcac gcctgtcgta cggagtgtta tgccatgacg gtccactatt
     1321 accggctgcg ggattacgcc ctgcagcatc cggaatgcag cgccattatg cgcatcattg
     1381 actgaatgga ggcagggaca atgcagcagc tttcctttct gcccggagag atgacgcccg
     1441 gcgagcgcag tctcattctg cgggccctga aaaccctgga ccgccatctt catgaacccg
     1501 gcgtggcctt cacctccacc cgtgcggcac gggaatggct gattctgaac atggcaggac
     1561 tggagcgtga agagttccgg gtgctgtatc tgaacaacca gaatcagttg attgccggtg
     1621 aaaccctctt cacaggcacc atcaaccgca cagaagtcca tccccgggag gtgattaaac
     1681 gcgccctgta ccacaatgcc gccgccgtgg tgctggcgca caatcacccg tccggtgaag
     1741 tcacccccag taaggcagac cgccttatca ccgaacgtct ggtacaggca ctgggcctgg
     1801 tggatatccg ggtgccggac catctgatag tcggtggcag ccaggttttc tcctttgccg
     1861 aacatggtct gctttaaccc gtcaccgcca caatcacatc acacctgttt tcacttttat
     1921 cttctgtctt cagaggtatc ccattatgaa aattatcacc cgtggtgaag ccatgcgtat
     1981 tcaccaacag catcctgcat cccttctatt tccgttctgc accggtaagt accgctggca
     2041 cggcagtgct gaggcgtata ccggtcgtga agtgcaggat attcccggtg tgctggccgt
     2101 gtttgctgaa cgccgtaagg acagttttgg cccgtatgtc cggctgatga gcgtcactct
     2161 gaactaatgt tccgtcaccc ggaaaacgag tacgaccaga tgacctcaga cgccgctgtg
     2221 gatgaggccc ttctcatcaa tgaatacatc ttcaccgaag ccggtctcca caccggctga
     2281 cctgtcatcg ttaacccctt cttaaccgat tttctgtgag gactttatcg tgtcagacac
     2341 gcaccatgag acaaattatc ccgacgacta taacaaccgc ccctggtggg ggctgccctg
     2401 caccataaca ccctgttttg gcgcccgtct ggtgcaggag ggtaaccggt tgtattacct
     2461 tgccgaccgc gccggtatca gaggccggtt cagcgacgcg gatgcgtacc atctggacca
     2521 ggcctttccg ctgctgatga aacaactgga actcatgctc accagcggtg aactgaatcc
     2581 ccgccatcag cataccgtca cgctgtatgc cagaggactg acctgcgaag ccgacaccct
     2641 cggctcctgt ggttacgttt atatggctgt ttatccgacg cccgaaacga aaaagtaaat
     2701 catcagaata accttctgct ccggcctggt gttttcacca cgccactttc ccatttttca
     2761 tctctgcata tcaggaaaat cttcagtatg aacacattac ccgacacaca cgtacgggag
     2821 gcatcgcgct gcccatctcc agtaaccatc tggcagacac tgctcacccg actgctggac
     2881 cagcactacg gcctcacgct aaatgacaca ccgttctccg atgaacgtgt gattgagcag
     2941 catattgagg caggcatttc actgtgtgac gcggtgaact ttctcgttga aaaatacgcg
     3001 ctggtgcgta ccgaccagcc ccggttcagc actagtcccc gctctcagtt aataaacagt
     3061 attgatatcc tccgggctcg ccgggcgacc ggcctgatga cccgcgacaa ttacagaacg
     3121 gtaaataaca ttacccgggg taagcatccg gaggcgaaac gatgaaactt tccctgacgc
     3181 tggaagccga cagcattaac gtgcgggcgc tgaacatggg gcgcattgtc gttgacgtcg
     3241 atggtgttga gcttgctgaa ctgattaacg tggtctgcga taacggtcac tcccttcgtg
     3301 ttgttgatga atctgaccgg gcctcaacag actgcacgcc accatttgct gccctgaccg
     3361 gcatacgctg cagtaccgcg catatcacgg caaaagacag cgcctggctg tactcgctgt
     3421 cacatcagac gaatgacttc ggcgaatcag aatggattca ttttaccggg tccggttatc
     3481 tgttacgtac tgatgcgtgg tcgtacccgg ttctgcgact taaacgcctg ggcctgtcaa
     3541 aaacgtttcg tcgtctggtc gtaacgctca tccgccgtta tggcgtcagt ctcattcatc
     3601 tggatgccag cgctgaatgt ctgccgggtt tacccacctt caactggtaa ccgggacaaa
     3661 agacacagta tggaatcatt aaccacagaa acggcgctgg atattctgat tgcgtggcta
     3721 cagaacaata tcgactgcga atccggaatt atcttcgaca acgatgagga taaaacggat
     3781 tcggcagcac tgctgccctg cattgaacag gccagggaag atatccgtac actgcgccaa
     3841 ctgcagtttc tgcaacagaa ccggtgagtc ttactcacca gacttcattc cactgacgcc
     3901 agcctgaata cggctggcgt tttcgtttat aaaccaaaag gaaaaacgat gatgtcagat
     3961 atcacagcct cccgtccgga agtggtcacc gggcatacgg acgttatttg ctcaaccagt
     4021 atccgccaca ttctggctgt acgaaagagt acactgctgc aaatcgacac acttatccgg
     4081 caactggctg aaatctcagc aatgacagaa agtattggcg gtaaaaccgc actggactgg
     4141 gcgatgaaac aggattttcg ctgcggctgc tggctgatgg agaaaccgga aaccgcaatg
     4201 aaagccatca cacgcaatct cgatcgcgaa atctggcgtg acctgatgca acgttccggg
     4261 atgcagagtc tgatggatgc gcagacccgg gacacatggt acaggtcact ggaatatgat
     4321 aattttccgg aaatcagtga agcgaacatt ctgagcacat ttgaacaact gcaccggagt
     4381 aaggatgaag tgtttgagcg gggagtgatc aacgtcttca aagggctgag ctgggattac
     4441 aaaaccaact caccctgtaa atttggcagt aaaattatcg tcaacaatct ggtgaggtgg
     4501 gaccagtggg gatttcatct tatcagtgga atgcaggcag atcgcctggc tgacctggaa
     4561 agaatgttgc atctgctcag cggtaaaccg atccccgaca accgggggaa tatcaccatt
     4621 aatctggatg accacataca gtctgttcag ggtaaagagg actatgaaga cgagatgttc
     4681 agtattaaat actttaagaa aggctcagta cacatcacgt tcaggaaacc agaactggtt
     4741 gacaggctta atgatattat tgccagacac tacccggaag ccctggcaag cacagtgaac
     4801 aaaagccgca aggcataaaa gaggtatttt acaattcgcg aatattaact attattaatt
     4861 gatagcaatc aatcggcgag aaaatcacca tgcctaccag tgtatccctc agtccttact
     4921 ttgaaacgtt tatccgcgaa caaatagaaa gtggtcggta taacaataca agtgaagtca
     4981 ttcgtgccgg acttcgtgct ctggaagaac gggaacagca gataaagctt gaatcactcc
     5041 agagcgccgt taccgcaggc atcaacagcg gtgaaagtaa aagtgctgaa gaggtattcg
     5101 ggcgactgac gcacaaatac aaaaagatgg cagaaggtga acagccaata tgaaactgac
     5161 tgtttcacca ttagcagaac acgatcttga agctataggt gactggatcg ctcaggataa
     5221 tcctgtccgt gcaatcagtt ttaccgaaga gctttaccaa caatgtctgt tgatagccga
     5281 atcgccagta ctctacagag aaagacccga attaggggct gggatcagag gttgttcgta
     5341 tggtcgttac cttctgctgt tcagggtact ggatactgaa gttcgtatcg aacgtatcgt
     5401 tcatggttcc cgggatatca acagggtact tgcagagcca gacgacaaac catcagatca
     5461 tttctggcag ccataaacaa aatctgtgcg ttaacgctcc ctaacacagg tagccaggta
     5521 ttttataaat ctctgtattt tgttgttact tcgattccgc aattcagcat atcgaaccag
     5581 acaaaatgat tacagcaaag cacaaaaaag agggattata gcgtcaaaag aattcgaatt
     5641 gcacatgggg gcctcattgg tggcctcaaa aacaacaaag acaaaacaat cgatacaaaa
     5701 caattggtta tataaccagt ttcattccga gtccgggcac cactattcta aagaacccgc
     5761 tcacaaggcg ggtttttgct tttggatctg acaataacct tcacgaaaca aattagctta
     5821 taaagcctgg gggaattact ctcgccacgt taacgagagt aattttattg atattaatct
     5881 cctgatactt tatccccgtc caaactccag ccgctgcaca ttcaccatcc cagtcttctc
     5941 agcagcactg acatcaattt gtgtcacccg cagcgcatat ttttcatcca gtgcgtttaa
     6001 ccatttcagc aggtcattaa acaccacagg ttctatccag acctgaatat tctccccacg
     6061 ctcggctatc cgcttgatga ccaccgagtg cgcggaagca ctgtcactga tgacccgcga
     6121 tacctgcgca ggcgttgttg tggcagattt tcgcgctgca ataatatccg gcgcggcgtt
     6181 cttcagtcgg gtgttcattg ccaccagctg ctgcaacatc gtctcctgtt gttctatccg
     6241 ttcgctcaac ggctgccaga tgaggacgta atatccgacg ctaaacagga acaccaccgc
     6301 tgccagtaac atgccctttt cacgcggaga acgccccgcc agatgttgtg tcagccagtg
     6361 ttcgccacgg cttaactggc gttcacgcca ttgctgaaaa tagtgaataa atttatcgcg
     6421 taacatgtta tttcctccgc aacgttacgc cgccggaaac cgcatcaccc tctttctgta
     6481 acgcgtcctg ttgcacaaca taatctgcgc cagtgcgcta cgcagtttgt cgaagctggc
     6541 aaagttcgca gcccgtagct ggaggtgaag cgtctggcgt ttttgatcaa aggtgaaacc
     6601 acgcatttcg atgtcggtaa gtgacgctga tttcagggta ctggcgatcg ctgacaattc
     6661 tgcgagcagc cgggtatcgt cggcctgcgg gcgatatttt ttcagcgcca tcgtcacctg
     6721 agagcgtaaa ttcacaatcc gcttctgctc cgggaacagt gttaagaact gtttctccgc
     6781 ctgggtgcgg ctttgcgcca cctgttcgct gacgctccat aacgtcacgc cccgttccac
     6841 tgccagcgca accagaatca gcaatatcgg cagaatcatc acccgccagc gcgcccactg
     6901 ttttcggtag ctgacgcggg gctgccacgg ccctgttagc aggttccctt ccggttcgcc
     6961 ataagtggta atggcgggca gagccgtaac ggtcaggcgt tcagcatctg ggccagccca
     7021 tgctgatagc tcttccggtg caatgccgac cacggttagt gaaagcggta aatcctgctc
     7081 attgagctgt gcgcggaaca tgaccggagc cagcgccagc ccggcgctcc atccccggca
     7141 ttcatcgatg cggcagataa cccgttgcgc atcgccagcc ataaacccac aaggaatgga
     7201 catccagtct ggcgcgacgg tagcgcgggt gatgccgttt gcctgcaacc actgcgcaat
     7261 gttgcgcata tgctgttggt gaatcacagc tacggttgcc agttgctggt cgattttcaa
     7321 cggggcgaaa tgcagttcat cgatatcctg gttcagctct tcttccagta aggcgggcag
     7381 aatagtcggt atctgcttgc gggcgacatc aggcagttca acctgccaga cgctgatcca
     7441 ttcgccggga atgtagagtc gaatcgcatc agtttgcagc cattgctgga gctgttcgtt
     7501 gtcaacgtca gaccagatgc cgtgctccac gtcggtggta cggtgctgcc aacggatggg
     7561 atcggaggcg caaagcggga taaaaatctc aagcatggaa ctcactcact ttctcctgtc
     7621 tgatgccaga gaaccgaaaa gtgttgtggg cccatgcgga caattaacga gttcatcgtc
     7681 agttcaatct cattcacggt gatatctgaa cgcagccaga agtaattgct gtccacgctc
     7741 aggacggttt ttagctgttt tttagtacgc tcatcgacgt cagcaagtag cggctgtgca
     7801 agaaactgat cgacatcttc ccagcccttc gccggacgtt gttgtaataa cgcccgcgcc
     7861 tgaacagggc ttaaccacgg gtcaaacagc gcctcaagaa tcacactttg cgcgacgtcc
     7921 agggtattaa tgttgatttg ctggcgggtc atcggcagcg cacagaccag cggtttcagt
     7981 ttttgataaa gtccggcgtc cattccctgc accacgcgca tctcgctgat atcagccagc
     8041 ggttgattag cggcgtaaaa cggcaccgaa cgggcgagat actcgctgtc ttcacggccc
     8101 agacgcgtct gcacgctgcg gtcttcgtca ataaactccc acaggctttc ggctatcagt
     8161 tcggcccgat aagcaggcac atccaggcgc gtgatcaggg caatcagttg ttgtaccgcg
     8221 agcggacgcg acgccgtcgt cggctgagcg agagcattca ggttaaagca ggcctgtgcg
     8281 tcacgcagag tgacggcgat ttgcccctgc ggcagtggga aaaaacgagg gccggaagcc
     8341 cacggctgcg ccagatgcac gcgcttttca tttttcaggc tcagactgag tgcgctcaac
     8401 gccaggcttt ccgcactggc gctgtaccac agcgcctgct ggtactcctg ctggctgcgc
     8461 gttcgcccaa gttgttgctg cattcgcccg gaaagcgtga tggtcaccag catcataacc
     8521 gccagcaata ccagcaccac gaccagtgcc attccgcgtt ttggtggtga ggtgatcatg
     8581 ataattgcgg cccgcgtaac aaccagatgc gttcaatctc gccccattgc ggcgaatgca
     8641 gagtcatgcg caccgccact ggtatcgcct gcactgatga ccagctctcc tgccagcgcg
     8701 tgccgtcgta gaactgcaaa cggagcgaat ccgccgggat taatttttgc gttgttggct
     8761 tcacgctgcc tgccgcatcg gtcagtggcc aggctaaccg ttcgagataa ccaccatgaa
     8821 tgcggtaacc gacggtgagc agattactgc gcggcagacg catcaacgga ttaaccacgc
     8881 cgccgcgcac aaaacgcatc ccttcactct cagacgccag cacgccagcg cccgccagta
     8941 acgctggttc acgctggccc tgatcgcctc ttaccggacg cggcatcatt tgtgtcagat
     9001 cgtggttcaa aaaactcatc gtttgctgca tgaggtttag tttttgatcg tgtccggcga
     9061 cggcgctatt cacgcgtgta acgccgtttg tcacctgctg cgccatcagt gccagtgagg
     9121 caaaaatggc aattgccacc agcatttcca gtaacgtgaa accagcgcaa gtccttctca
     9181 ctgttggcct cccacggcgc taaaccaggc gcgtcgtgac tgaatcaccg acgaaaagtc
     9241 ttcgtgacgg ctgacttcaa tatccactgc ctggagcagc gcgttgcctg tactcagcgg
     9301 tgtggttcgc cagtaccagc ggcttcctgc catatcactc tcgccctggt gcctgaccgc
     9361 tcgttgttca cgggtcatca ggtaaagcag tgcggtctgg ttttcagcta cccagcccgc
     9421 taccgttttt tcttcaagga tttgctgatt actcagcgcg ccgctggcga tctgtaacac
     9481 cgccatggcg gccaacgcaa aaatcgccag cgcgagcatt acttccagca aggtaaatcc
     9541 acgcttcatg gcatcccctc atcacattga ttgagggata acacgccatc gtgcgccagc
     9601 tttaccgccc aacacgcgtt ttgtgccgac gcgtaaaaac gcagtgtaaa cggcgtggcg
     9661 ggttcaaaag gcgaaaaacg gatctgcggt gtcttctttt tcgcctcttt ttgcagctcc
     9721 agttcaatat cgtgcagcgt caggcgacga cgctgcagct ccagcgcata ttccttctgc
     9781 cagatatcac ttccgggttg taacagcatc tgcacctgtt tcggcaccgt aacctgtgcc
     9841 gataaccggg tcgcagaaac gggcaaccac tgcccgtgac gacgctgcat aaactggtaa
     9901 cctggtgggt cgattagcac accgagtgtt ttcccttcga tcactgccct gtccttaaac
     9961 tgcgcaaagc gagtcagaaa agcctgcgcc gcttttttcg caggcggctc tgaagcggtc
    10021 gcaaacgtct gtaccacgcc cgcactggca aggccgataa ggaaaatcac cagcatgatt
    10081 tccagaagtg tgaatccgcg ttcaggcact gattactgaa actcctgcaa attccagtta
    10141 ccgatatctg cgccagcgcc ctccccgttt tcctgaccat ctgcccccag ggtatacaca
    10201 tcaaacagtc ctttttcacc cgggctgaga tactgataat cattgcccca cggatctttt
    10261 ggcagtcgtt taatgtatcc accggtacgg tagttgcggg agtccgccat attggccggt
    10321 tgctggatca gcgcctccag tccctgttcg gtagtcggat aacgcccgtt atccagtcgg
    10381 tacatatcca gcgcattctc cagcgccacg atatcgctga tggctttttg ccgatcggct
    10441 ttctctttgt tgcccagcag gttaggcacc accagacttg ccaggacgcc aagaataaca
    10501 atcaccacca tcacttccag cagggtaaaa cctgcccgtg gtttttgtgt gcgggataac
    10561 gaattcatgt gataactccg taaattacat tccaaccata ttgttcagtt gcagcatcgg
    10621 ttcgaggatg gcgatgacga taaacagcac cacgcccgcc atcatcacca ccagcgccgg
    10681 ctcaaacagc cctaacgcca gccccacctg ggtatcaaat tctcgttcct ggttaactgc
    10741 ggcctgttca agcatggttt ccagttcacc gctctgttcg ccggaggcga tcatgtacag
    10801 catcatcggt gggaacaggc gcaactccgc cagcgcagcg cgcagactgc ttccttcgcg
    10861 gacgcgatct gccgccagca gcagttgttg ttcgacatag cgatttgccg acacggcggc
    10921 agcggtctga atgccttcca gtagcggaac actgctggcg gtgaggatgc ttaacgtgcg
    10981 ggaaaaacgc gccgtattca gcccgcgcgc aacacgcccc gtcacgggca aacgcagcaa
    11041 ggttttatcc cagcgtaggc gcatggcagg atttttgagt agccgttgcc ccagcaccag
    11101 aagccccagc aatcccgcca gccagtaaac gccgctggtc tgtaacgtgt cgctcatggc
    11161 gatgagcgtg cgggtggagg caggcagcgc gtgtccgaga tgatcaaact gttcgatgat
    11221 tttcggcacc actgccgtca gcaaaatagt gactacgccc gttgccacca ccagcaatac
    11281 cagcggatag agcatggcct gcaacagtcg tgatttcagg cgctgtcgct gttcggtgta
    11341 atccgcaagg cgattgagca ccacgtcgag atgcccggat ttttctccgg cagcgaccat
    11401 cgaacaaaac agggaatcaa agacgcgggg atgttcgcgc aggctgtccg acagggtata
    11461 accttcctga atccggctgc gcagcgccat tccgaggctt ttcacatgca gtttttcact
    11521 ttgctcactg accgcctgta agcaggtttc cagcggcatt gctgcctgca ccagcgttgc
    11581 cagttggcgc gtgaacagcg ccaaatctgc cgccgccacg cgacgatgtg cgtgccgccg
    11641 acgctgcaac attccccctg tcgaagcatt cagccgggct tcaatgtgca cggggataag
    11701 ctctttacca cgcaacaact ggcgggcatg acgcgcggaa tccgcctcaa tcattccttt
    11761 ggttttgcga ccattacgct ccagcgcctg atagtaaaac agtgccatta cgcctccata
    11821 gttacccgca gaacttcatc gagagaggtt tctccggcga gcactttctc aatgccgttg
    11881 ctgcggatgc ccgcagagtg ttgccggaca taacgttcca gctccagttc cccggcctga
    11941 cggtggatca aatcacgcaa tgtggcgtcc accacgatca actcatggat ggcagtacgc
    12001 ccgcggaaac ctttgtgatt acaggcgggg cagccctgcg gatggtacag cgtgacagta
    12061 cgggcgtcgg taatccccag caggcgtttt tcttcgtcag tggcaggcgc gggctggcgg
    12121 cagtcggggc acagcgtgcg gaccagtcgc tgcgccatca cgcccgtcag gctggaagag
    12181 agcaggaaag gctcaacgcc catatcctgc agacgtgtga tcgcccccac cgccgtgttg
    12241 gtatgcagcg tggaaagtac caggtgcccc gtgagcgacg cctgaacagc gatttctgcg
    12301 gtttctgtat cgcggatttc accgaccatc accacatccg ggtcctgacg caaaatcgcg
    12361 cgcaggccac gggcaaaggt catgccgacg cgggtgttga cctgcgtctg accgatccct
    12421 tcaatcatgt attcgatggg gtcttcaacc gtgagaatgt tgcgcgaatg gttgttcagc
    12481 tcctgcaatc cggcgtacag cgtggtgctt ttgccggaac ccgtcggtcc cgtcaccaga
    12541 aagatgccgt gcggtttgtg taacagctgg cgcaactgca cggtcagttg ctgactcaac
    12601 cccagacgtt ccagcgtcag gcgggcctgg tttttgtcca gcagtcgcag caccacgcgc
    12661 tcgccccagg cggagggcat ggtggagaca cgcacgtcaa tcgcccgacc gcccagcagc
    12721 agcgcaatac gcccatcctg cggtacgcgc ttttcggcaa tatccagccg cgccatcacc
    12781 ttgatacgcg ataccagcag cgaggccagt ttgcgccccg ggcgcagcat ttcatgtaat
    12841 gtgccgtcaa cacgaaaacg gatcaccaga ctcttttcaa atgtctcgat gtggatatcc
    12901 gaagcgcctt ctttaatcgc ctctgccagc atggcgttga tcagtttgat gattggcgcg
    12961 tcgtcgtcgc tttccagcag atcttccgtt tccggcagtt cttcggcgag ggtaaaaaaa
    13021 tcctcagcag aaccgagatc ttccatcagc tggcgggcct cggaagagtc ccgctgccag
    13081 accgcattca gccgctgttc aaattcggct tcgtcgattt gccgcagcgt aaagggcgcg
    13141 ttcagccccc gctgcagctc ctgcaaaaca gagagcgaca acggatgaac gtggaggatc
    13201 tccagcgacg cttcgcacca tgccaccagg ctaaaccgac ggctgaaact gtagggcaga
    13261 cgcacggtgt tagcggtggt ttcctgtgct acaggcacca ttaacgcgtt ctcccggcat
    13321 tgaggaatgc gcgaacttcc ggcggtaagg cctggttttg cgctggcagt accggctgcg
    13381 cggtgtgcgg catcaggctt aagccttgat catcgcgata gatctgctcg gcgcgcatgt
    13441 agttatattt gcgctgcgac acgccgtctg ccgccatacc gtcacgcaga atggtcgggc
    13501 ggataaacac catcaggtta cgtttttctt ttttatccgc cgtcgattta aacaggttac
    13561 caatcaacgg gatatcgccc agcagcggca ctttcgccac gctttctccc gcctggtcgt
    13621 ccatcagacc gccaagcacg atcagttcac catcgttagc cagcacggtg gtcttcagtt
    13681 tgcgctcacc aaacaccacg tcgaggctgg tctgtccttc caccttcgac acttcctgct
    13741 caatcaccat ctgtaccgcg tttccttcgt taatctgcgg cgtgactttc agcatgatgc
    13801 cgactttttt cctctctacc gtattgaaag gattgctgtt attggagcca acggtagatc
    13861 cagttaatac cggaacgtcc tggcccacca tgaagaaggc ttcctggttg tccagcgtgg
    13921 tgatgctcgg cgtggagagc acgttcgagc tggagtcgtt tttgaccgcc tgtaccagtg
    13981 ccatccagtc gcctttcacc acgccaaccg ccgtaccgct aaagccagaa agcagctggg
    14041 cgagcgtgga gagatcgccg ttggtatccg ggttaatggt ggtggcaccg ttttcactga
    14101 tcaccgtgga gcctttctgc ggtttcgcct gagaaatggc tgcccccagc gtgccgatag
    14161 ggatctgcgt accgttagca aactgcatta atccggcatc tttcgacgcc cactgcacgc
    14221 cgaaattgat attgctgcct tcagcaactt ccacgatcaa cgcctcgacg tgcacctgag
    14281 cacggcgaat atccagttgt tcaatcacgc tttgcagcga ctgcatgatg tcctgcggcg
    14341 cagtaacaat cagggcatta ctgtgtttgc tggcggcgat ggagacaacc tcacgcccgc
    14401 taccgacggt gccttccgcc tcttctttag ccgccgtgag cgtgccgctg acctgcttca
    14461 gcacatcgac cagatcttcc gctttgctgt atttgagata gaaaacctgg ctgttgccgc
    14521 tgcgctccat ttctgagtcc agccgacgga tcaggcggcg cattttgtcc cgcgtggccg
    14581 ggtcaccact gacaatcaca ctgttggtgc gttcgtcggc gacaatttga gatttcagcg
    14641 tcgcaggttg gttctcgccg ctgtttttcg tcaggctttc cagcacgcgg gcgatttccg
    14701 aagccgaggc gttatccagc gggatcacct cttcagtgcg attacccgcg tgatccacac
    14761 gctggatcac ttccgtcagc cgctccacga cggaggcgcg cccggtgagc ataatcacgt
    14821 tggagggatc gtaattaaca acgttgcctg agcctgcgct gtcaatcatc tggcgcagaa
    14881 tcggtgccag ctcgcgtacc gacacattgc gtaccggcac gactttggta accatttcat
    14941 cgcccgcgta gttgtcgctg ccttcaccga ccagcggcag cggctcgact ttcgcggcgc
    15001 ttgatttcac caccttcagc acgtcgtttt ccatcggtac gaccgcatac ccctgtgctt
    15061 ccagcaggtt aaggaataac tggtaatact ggcgttcatt gagcggagtc atagtgcgaa
    15121 tactcacttt cccctgaacg cccggcccca taatgatggt tttattaagg ttagcgccga
    15181 cggtttcgat gaacgatttc aggtcagtat ctttaaaatt agcggtgaaa gtggcttctt
    15241 ccgcccagac cggtgaacta cacaatgccg ctgccagcat cagcggcagt aaacgctttt
    15301 ttgttttgag gccagttgtc ttcttacgcc agatagacaa cgtcatatca cgccaaaaca
    15361 cgatgaatga ttctcctgga ttaaatgcgg ttagcgcagt gcgatggaaa tgtcgtagcg
    15421 cgcacccttg cgtaaaaccg taagttgaat ggaatccatt gaaggtaact gccgcatcag
    15481 agcaatcatt gctcgtggat cagtgaaatc ctgctgattt agtgcaatgg cgatatcgcc
    15541 ttccctgaaa ccgctggcat cgaacagaga acgatctgcc cccggcttca ctgcataacc
    15601 gacaattccc tccttacgca caggcgtaag ctggatatag ttaaaaattt tctgcggatc
    15661 tttcgccagt gcctgacgca cggcagccgg gatctcaact ggcgcactgg cagcaggctc
    15721 agcaacagct tgctttgctt cgtcgctgac ggcttttttg ctggtgaccg ccacaggggg
    15781 gcgttcctcc tctgccagac tcagacgttc cattttcccc tgatagcgca gcatcacatg
    15841 gtcgcggttg atttcctcaa tcactgcgtt gtgagagcca agccgttcac cctgcaaata
    15901 gacctgctgt ttgccgcctt cttcaatcac cgcgccgggt ctggcaccaa aggcgatccc
    15961 acgcagcacc acattaagac gcgtttctgc cacaggcgca gattcaggtt gttttaccgg
    16021 tgcggcgacg ggttgatatt tgccaaacca gttttgttgg ctgactaatt gcacgtcatt
    16081 tttatcgaac ggttttgcat ccgcacggag cggtttattc gccgatgagg aaaccaccgt
    16141 atattccgca gaaaaggaga aatagcgcca gagtgaatac gccatttttg cagaaataat
    16201 aagcagcatg agccagaaca tcccgcgcac catttttcgc agatgctctt tgtctgtatt
    16261 aagagattgt ctctggctaa gaatgtgacg aattttagtc agccattgaa tgagataaat
    16321 tcgtgcgtca cgaaaaacaa cccgcgccaa tttatatcta cccgacgtta tgctttgact
    16381 attccacagg tggtacgatc cagtttccgc tgatactggt tacctgtacg tgtatcatta
    16441 atcgttatgc tgtaattaat tccctcggtg atcattaatt tcaccgttgg gtcagcgcac
    16501 atttgtcgtt gatagctggt taaaaaggcg tcaggtgtct gcgtggtctg agtacccgat
    16561 tcgctgataa tggtcatttt taccgtcgtg ccactacttt gcgccagcac taaggtatat
    16621 cccgcagatt taatcggcag gttttggctg atattttgcg cctgtttttt cgccagtaaa
    16681 ctggcgtttt cattatggct ggcgcagcca cttaataatc ctgtaacgct caacaatagc
    16741 gatattaata ccctccctgg catttgtttt atcgacatgg taaataatct ctaaggttat
    16801 taataagagt taaaatgtca ctttgataat gacgttgtta tcattaaaac aatgccttta
    16861 gataaagtgt tgctataccg cccagactta aacacggtcc aaaaggcagt gtggttgatc
    16921 ctctttttgt aataacggca tatatcaggc cgcagcatga ggcgattaaa gcaacgttgg
    16981 gcagtgataa cgcccccacc cagccaccta aagcggcgaa gagtaataca tcgcccatgc
    17041 ctaatgcttc tttacgcaga actattccgg ctatccagcg cagggagtaa aaagtgataa
    17101 atcccaccag tacgccggtg actgcgtctt gtagcgtgag cggactctgc tgcgcccatg
    17161 ccgcaattag tcccgtccac aatacgccct gagtaaaaac atcgggcagc cattggttat
    17221 cgaggtcaat gacgctcgcg gcaatcagcc aggcggataa tatcatcacc gccagcgccc
    17281 atccactttc cggccacacc aggctcgcca gcaaaaaagc gagtgccgtc aataactcca
    17341 ccagcggata acgcttgctg attttcgcct gacagtcgcg gcagcgccct ttgagcatca
    17401 accaggagag cagcggaata ttgtcacgaa cgcggatagt ctgctggcaa tgcggacagt
    17461 gcgagcgtgg cagcgccagg cttatttttg actgcgtact cggcatttca ccgtgaaact
    17521 ccgccatttg ttggcgcagc atgatggggt aacgccaaat caccacattc aaaaaactgc
    17581 cgataatcaa tcctccgacg gtagccagga cgggcatcgc cgcggggtat tgctgaaaaa
    17641 catcaaaaaa catggttaaa ggttatttgt tgtaacttgc cggatgcggg cctgcaggtg
    17701 tatgccatac ggctttcctc caggcccgat gcgccttatt tcatgccgga tgcggcgcga
    17761 gcgccttatc cggtatacag gcttactcag ctgacatctt atgctcggta acctgattaa
    17821 tggtttccgg cccttgctgc ggtttcggca gattaagcgc cgccagcgtc tcgtaagccg
    17881 actggctcac accgccctcg aagttcatct cgctcgctcc cggcaactgg taagcattcg
    17941 cgcccggatt ccatttctta aagaactccg aaagatccgt ctgggcgacc caggaggcac
    18001 acagcatcag cgtgtccgct gcgttaccgt tggattcagc acagtagttc ttactgccga
    18061 acttgttatt gccaacctca tcgccgcgcg ctttacggtg catcaactgg aacaggttcc
    18121 agcctttcat cccttcacgc tcgctgtaaa acttaggcag gtcaccttct gggtaccact
    18181 gtttgatatc aaagtttttc tctgcccact ctttcagctg tgcgtacatc agcagacggt
    18241 cccccgcacc gccgcgcgcc caggcctgac cgttgctctc gtccagatat tccggcgcga
    18301 cggtaatgtc gtcagcgaca cggttcatct tgccgagata gcgatcctgc atgtacagcg
    18361 ccagcacgtt gttcgccact tcagttgcac ccggtacgtt cagcggtgtt tctgcagcgt
    18421 tatgaccggc ttcgtgccaa atcagccagt cgttcagcgg cgttgtcggc agcgtggtgc
    18481 tgttcgtcga gaagctgctg ttcattaccg gataacccga gtgcgcatca ccgatggaga
    18541 tctgcacatc gttggcgaaa cggtgtttat gccccgtcaa tgcttcatag gtaaacatcc
    18601 ggtggttacc gctcgtttca ttgcgaccgt agaagtcatt catcgagctg gcaaaggtat
    18661 ccagatcttt agcgaattct gctacaccac cagtgaaatt gctggcctca aggttcttct
    18721 tcggcgtggt atagacgaac gacgcagact ccagctcgcc cagcggcgcc ggtgagtcca
    18781 gatcgttttt ccattcgccg tctttataga acggcgcttt tactacaccg gtaaaggtga
    18841 agttagcaga aacatcatcc ttactgtcgc ccttgatata aatcagacca ccataaggca
    18901 ccttgaaggt cacttcaccg ttagcctcca gagtatacgt tttagtcact cttggcggac
    18961 ggttcagcgc aacttcatgc ttctcacgtc cagtcaggtc gtcagccagc gccacggtaa
    19021 cagtcactgg gactgacgcc gaagacttaa tggtgacgtc ctgctgggcc ggtgcccaca
    19081 ggccggttga ctgcatgtta cccgcaaacc atttggtcgg attcgagtac aggctgatgt
    19141 tttccgtaac gctctcaccc tttgccgata cggatcctgg gtacttctcc acatcaacct
    19201 taatgttcag atcccaccag gaacggccca gcatcagacg cgtcagcggt ttttccatat
    19261 agttgagcgg atagcttggg ttcatcatgc ccgctttgct gctaccgtca ccgtagatca
    19321 tgttgttatc gaccagcgat tttttcagat ctgcggagca cttggtgccg cctgcatagg
    19381 catcattggc gtagcagttc aggaactcgg tgaacgtttt aaagcccagc tcatcttcct
    19441 tgccctcttc gtaacgatat ttcgtatcgt tccacagcca gaccgacatg ttctggtaca
    19501 gacgttccag atcaacactg ttcagacgct cacctttact gcctttggta cggaaataaa
    19561 gctcatgctg atacaggcgc tgaatgttgg tgcctaaatc cgccgcctgc accatcgctt
    19621 tcgcggtgtc ggcgtcaaga ttcagttgcg tatagcgcgg aacatacatg ccacctgtta
    19681 ccggaacatc cgtgcctggg cggcgctcca aacagttaat ctcgtaatgg taagtcgagt
    19741 ccttacactc ctgtaaccca ggaaacttct caaagatttt tgcctttgcc gcttccagag
    19801 attcttctgt tgtgtattcc gcttcatcaa taaaggcata acgcgttacc tgtttgccct
    19861 caacttcctc ctgccagctc gcaacttcca gtttcggctt gtcatcaggc ttgttgtctt
    19921 gctggtattt ccaggtcact tcccctgtat ttgggtcgat ggtgtacggc ggttgcgcgc
    19981 cgtctgcagc aggataacgt tcataaaccc aaatgccagt cgcgcgacgc tgacgaacgc
    20041 gatccggata cccttgcgga tcgttgttca ccaccgattt gttcagagcc attgacagac
    20101 ccgcggcatc cagcagacgc acaaaactgg acgcgctctc ttccttaaga ttgctcatca
    20161 cgttttccat gatcagcacc gagccacctt tgttcagata agcgatcaga tcggtcacat
    20221 cctgctgagt cagcttcggt ttgctggtat ctgcacgcag aggcacagca tagggatcgc
    20281 cagaccactg agtcacatat tcaaagccgt tgaggatcag caacggaatc tcttccggat
    20341 tcagatcgcc atagctggtc aactgtttaa ccgtgatacc agtgaaatcc tcatggaaag
    20401 caaatggtgc actatttccc aatacctggc ccgctttttt gaaataaacg ttctccaggt
    20461 tggtgccgac agtcatgatg ctcttggtat ttggctgcca gatgtcattt gacaagtagc
    20521 gcaggacgtt ctgcataaag tgcttcatgt catcagaatc accgctgagc gtacactcac
    20581 ctttactatt aacaccaccg ccccaactgt aaccgttcgg gcaacgcagg atgctgttgt
    20641 agtgcgggtt accgataacc atcagtttgc cttcaccgac ttgccccagc gaaataaacg
    20701 gcaggttgaa agtcgcagta tcgcgcgtaa cgttctctgg ctgcacaatg gaaggcgctt
    20761 ccgtaatgta cgccagctca tttttatccc aggcgcgttt ttcgccaaac gccagccagt
    20821 agtttttatc attacgcgcc atcagaatcg ggaatgccga gttggagata tttaccaccg
    20881 cctgaccgcg cgcgttaccg gtgctgccat agaagttggt agagtcatgg aagacgtgga
    20941 acttgctgac agactgatag ttcgtatcca cgccccacag cttgttaata acgccctgaa
    21001 tctggccgtc attaacattg cgcgttgtca gcgagaacca gcgagcctcg ttacaaccgt
    21061 cggttttcgc acaaatcgcg gtatcgatct ctttggcctg acccgtctta aactgctcga
    21121 taaattcgtt aggcagcaca acgttttgat cgccttcatc cagcgtcgca ccgttggata
    21181 acgaaagatt gattatctcg ttgatcacgt tgggatattc ggcaaagacc ttgcgtacat
    21241 agtccggaac aacacgagta ttattttgac cagtcgtcga ataacgatga atgagctgat
    21301 cgatatttgc cccgcgaact tcatcaccca attcagtcag cgcaatggtc gacttattgc
    21361 cacgtactga gcccagttca aaggtatcga taccaaagga gatgtttcgc cccagctaaa
    21421 ggagaattta ccgttttcgt cagttacgcc acggcctgaa ttggtgtagt agtcaacgcc
    21481 agcaacaccg ttccccaggc tatccaccag ttggccttcg gaaagaatga tttcagtggg
    21541 ttgatactga taaaactgtt ccgcgttagc cgacacgaag gacgcgttca gatccggttt
    21601 tgttccctct gtcgtgactg gcactaccgt tgaggtatgg gtggacggcg ctttatcagt
    21661 cgcagcattg ttttccacct cttcattgac cagcttgctg aaattgtctg ttgccagatc
    21721 aatttgctta tacagttttt caaatcgcgc gcgatcaacc actgacgaga aagtaagaca
    21781 aagctgttct gcatctgcgg ggcaactgtc gctggacgtc accagagaga tggcgttggt
    21841 tttcttattt tcagaattcg ccagctcctg cgcgtcctcc aggctaaacg acactttgtc
    21901 aaccgcacgc aggctacgcg cagcttctga ctgggtgttg aatgttgcaa tggtcgtact
    21961 gcccaccaca caactcacgg tattgcctgg cgtaaaggta aagccatcgc tggattcacc
    22021 attacaggta gcaccagtta cccgctggct tccgcccagg gtcagataac ccgttttcgt
    22081 aggaacaggt tctggctccg gttctggctc tggatcaggt gtcggttccg gcgtaggttc
    22141 tgggtccggc gttggctcag gcgtcggctc cgggtttggt gttggatcag gtttcacttc
    22201 cggcaaagac cctgttccag aatctacagg cggcgtatcg gaggaagatc cggaaccacc
    22261 gccatcacaa ccggctaaca ggattgcgct caaaatagcc gctaaaagcg atttcttata
    22321 tttaaatttc ttattcatta ataacgcaag tgacaaaaca atgttaa
//
LOCUS       DAZKGF010000078        22159 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZKGF010000078 DAZKGF010000000
VERSION     DAZKGF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.078"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            41..2392
                     /gene="polB"
                     /locus_tag="ACHOQV_002769"
     CDS             41..2392
                     /gene="polB"
                     /locus_tag="ACHOQV_002769"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFP9631117.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQVKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGSIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            2556..5462
                     /gene="rapA"
                     /locus_tag="ACHOQV_002770"
     CDS             2556..5462
                     /gene="rapA"
                     /locus_tag="ACHOQV_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFP9631118.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            5474..6133
                     /gene="rluA"
                     /locus_tag="ACHOQV_002771"
     CDS             5474..6133
                     /gene="rluA"
                     /locus_tag="ACHOQV_002771"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFP9631119.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6251..7066)
                     /gene="djlA"
                     /locus_tag="ACHOQV_002772"
     CDS             complement(6251..7066)
                     /gene="djlA"
                     /locus_tag="ACHOQV_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFP9631120.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKLQKGFK"
     gene            7321..9675
                     /gene="lptD"
                     /locus_tag="ACHOQV_002773"
     CDS             7321..9675
                     /gene="lptD"
                     /locus_tag="ACHOQV_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFP9631121.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            9728..11014
                     /gene="surA"
                     /locus_tag="ACHOQV_002774"
     CDS             9728..11014
                     /gene="surA"
                     /locus_tag="ACHOQV_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFP9631122.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11014..12003
                     /gene="pdxA"
                     /locus_tag="ACHOQV_002775"
     CDS             11014..12003
                     /gene="pdxA"
                     /locus_tag="ACHOQV_002775"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFP9631123.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDKLRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            12000..12821
                     /gene="rsmA"
                     /locus_tag="ACHOQV_002776"
     CDS             12000..12821
                     /gene="rsmA"
                     /locus_tag="ACHOQV_002776"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP9631124.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDKLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            12824..13201
                     /gene="apaG"
                     /locus_tag="ACHOQV_002777"
     CDS             12824..13201
                     /gene="apaG"
                     /locus_tag="ACHOQV_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFP9631125.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13208..14050
                     /gene="apaH"
                     /locus_tag="ACHOQV_002778"
     CDS             13208..14050
                     /gene="apaH"
                     /locus_tag="ACHOQV_002778"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFP9631126.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKHYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(14128..14607)
                     /gene="folA"
                     /locus_tag="ACHOQV_002779"
     CDS             complement(14128..14607)
                     /gene="folA"
                     /locus_tag="ACHOQV_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFP9631127.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(14799..16661)
                     /gene="kefC"
                     /locus_tag="ACHOQV_002780"
     CDS             complement(14799..16661)
                     /gene="kefC"
                     /locus_tag="ACHOQV_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFP9631128.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGAVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSSEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(16654..17184)
                     /gene="kefF"
                     /locus_tag="ACHOQV_002781"
     CDS             complement(16654..17184)
                     /gene="kefF"
                     /locus_tag="ACHOQV_002781"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFP9631129.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(17292..18623)
                     /locus_tag="ACHOQV_002782"
     CDS             complement(17292..18623)
                     /locus_tag="ACHOQV_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9631130.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(18682..18969)
                     /gene="fixX"
                     /locus_tag="ACHOQV_002783"
     CDS             complement(18682..18969)
                     /gene="fixX"
                     /locus_tag="ACHOQV_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFP9631131.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(18966..20252)
                     /locus_tag="ACHOQV_002784"
     CDS             complement(18966..20252)
                     /locus_tag="ACHOQV_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9631132.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(20303..21244)
                     /locus_tag="ACHOQV_002785"
     CDS             complement(20303..21244)
                     /locus_tag="ACHOQV_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP9631133.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(21259..22029)
                     /locus_tag="ACHOQV_002786"
     CDS             complement(21259..22029)
                     /locus_tag="ACHOQV_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFP9631134.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         5330 a         6147 c         5787 g         4895 t
ORIGIN      
        1 caggctataa tcaagcctgg ttttttattg gattttcagc gtggcgcagg caggttttat
       61 cttaacccga cactggcggg ataccccgca aggaaccgaa gtttcctttt ggttagcgac
      121 ggacaacggg ccgttgcagg ttacgcttgc gccgcaagag tccgtggcgt ttattcccgc
      181 cgatcaggtt ccccgcgccc agcatatttt gcagggtgag caaggctttc gcctgacgcc
      241 gctggcgtta aaggattttc atcgccagcc ggtgtatggc ctttactgtc gcgcccatcg
      301 ccagttgatg aattacgaaa agcgcctgcg tgaaggtggc gttaccgtct acgaggccga
      361 tgtgcgcccg ccagaacgct atctgatgga gcggtttatc acctcaccgg tatgggtcga
      421 gggtgatata cgcaatggcg ctatcgttaa tgcccgtctg aaaccgcatc ccgactaccg
      481 tccgccgctc aagtgggttt ctattgatat tgaaaccacc cgccacggtg agctgtactg
      541 catcggcctg gaaggttgcg ggcagcgcat cgtttatatg ctggggccag agaatggcga
      601 cgcctccgcg ctcgattttg aactggagta cgtcgccagc cgcccgcagc tactggaaaa
      661 actcaacgcc tggtttgcga ctcacgatcc cgatgtgatc atcggctgga acgtggtgca
      721 gttcgatctg cgaatgctgc aaaaacatgc cgagcgttac cgtattccgc tgcggctggg
      781 gcgcgataac agcgagctgg agtggcgcga gcacggcttt aaaaacggtg tgttttttgc
      841 tcaggttaaa ggtcggctaa ttatcgacgg tatcgaggca ctgaaatccg cgttctggaa
      901 tttctcttca ttctcgctgg aaaccgtcgc tcaggagtta ttaggcgaag gaaaatctat
      961 cgataacccg tgggatcgaa tggacgaaat tgaccgccgt ttcgccgaag ataagcctgc
     1021 gctggcaact tataacctga aagattgcga gctggtgacg cagatcttcc acaaaactga
     1081 aatcatgccg tttttgctcg aacgggcgac ggtgaacggc ctgccggtag atcgacacgg
     1141 cggttcggtg gcagcgtttg gtcatctcta ttttccccgt atgcatcgcg ctggttatgt
     1201 cgcgcctaat ctcggcgaag tgccgccgca cgccagccct ggcggctacg tgatggattc
     1261 gcgacctggg ctttatgatt cggtgctggt gctggactac aaaagcctgt acccgtcgat
     1321 catccgcacc tttctgattg atcccgtcgg gctggtggaa ggcatggcgc agcctgatcc
     1381 agagcacagc accgaaggct ttctcgatgc ctggttctcg cgagaaaaac attgcctgcc
     1441 ggagattgtg actaacatct ggcacgggcg cgatgaagcc aaacgtcagg gtaataagcc
     1501 gctttcgcag gcgctgaaga tcattatgaa tgccttttat ggcgtgcttg gcaccaccgc
     1561 ctgccgcttc ttcgatccgc gcctggcgtc gtcgatcact atgcgtggtc atcagatcat
     1621 gcggcaaacc aaagcgttga ttgaagcgca gggctacgac gtgatctacg gcgataccga
     1681 ctcaacgttt gtctggttga aaggcgcaca ttcggaagaa gaagcggcga aaattggtcg
     1741 tgcactggtg cagcacgtta acgcctggtg ggcagaaacg ctgcaaaaac aacggctaac
     1801 cagcgcactg gaactggagt atgaaaccca tttctgccgt tttctgatgc caaccattcg
     1861 cggagccgat accggcagca aaaagcgcta cgccggactg attcaggagg gcgacaaaca
     1921 gcggatggta tttaaagggc tggaaaccgt gcgcaccgac tggacgccgc tggcccagca
     1981 gtttcagcag gagctgtacc tgcgtatctt ccgcaacgag ccatatcagg aatacgtgcg
     2041 cgaaaccatc gacaagctga tggcgggcga actggatgcg cggctggttt accgtaaacg
     2101 ccttcgccgt ccgctgagcg aatatcagcg taatgttccg cctcatgtac gcgccgctcg
     2161 ccttgccgat gaagaaaacc aaaagcgcgg tcgccccttg caatatcaga accgcggctc
     2221 cattaagtac gtatggacca ccaacggccc ggagccgctg gactaccaac gttcaccgct
     2281 ggattacgaa cactatctga cccgccagct acaacccgtg gcggagggaa tactcccttt
     2341 tattgaggat aattttgcta cacttatgac cgggcaactt gggctatttt gagcaaaaaa
     2401 agagttcgcc agataccatt ttgatgcgtg acgaatgctt tgccatccag taccatagcg
     2461 ccctttccat tcctggacct gaataacacc actacctcat aagcacggta gcgggtggtt
     2521 attgcctgca attaaagata tagagccgaa cacatatgcc ttttacactt ggtcaacgct
     2581 ggatcagcga tacagaaagc gaattgggac ttggaaccgt tgtcgcggtg gatgcgcgaa
     2641 ctgtcacttt acttttccca tctactggtg aaaaccgtct gtacgcacgc agtgattccc
     2701 ccgtgacccg cgtgatgttc aaccctggtg atactattac cagccatgac ggctggcaga
     2761 tgcaagtcga agaagtaaaa gaagaaaatg gcttgctgac ctatatcggt actcgcctgg
     2821 atactgaaga gtccggcgta gccctgcgtg aagttttcct tgatagcaaa ctggtgttca
     2881 gcaaaccgca ggaccgtctg tttgccgggc agattgaccg tatggaccgc tttgcgctgc
     2941 gttatcgcgc gcgtaagtat tccagcgaac agttccgtat gccgtacagc ggcctgcgcg
     3001 gtcagcgtac cagcctgatc ccgcaccagc tcaacatcgc tcatgatgtt ggccgccgcc
     3061 acgcgccgcg cgtcctgctg gctgacgaag tgggtttagg gaaaaccatt gaagccggga
     3121 tgatcctgca tcagcaactg ctctctggcg ctgctgaacg tgtgctgatt atcgtcccgg
     3181 aaaccttaca gcatcagtgg ctggtagaaa tgctgcgccg tttcaacctg cgctttgctc
     3241 tgtttgatga tgagcgttat gccgaagctc agcacgatgc ttacaacccg ttcgataccg
     3301 aacagctggt gatttgctcg ctggattttg ctcgtcgtag caaacagcgt ctggaacatc
     3361 tctgtgaagc cgaatgggat ctgctggtgg tcgatgaagc ccatcacctg gtgtggagcg
     3421 aagatgcgcc gagccgcgaa tatcaggcca ttgaacaact ggcagagcac gtgccgggcg
     3481 ttctgctgct gaccgcaacc ccggaacagc tggggatgga aagccacttc gcccgtctgc
     3541 gtctgctgga cccgaaccgt ttccacgatt ttgcgcaatt cgttgaagag cagaaaaatt
     3601 atcgtccggt tgcggacgcc gttgccatgc tgctggcagg taacaaactg agcaatgatg
     3661 aactgaacat gctcggtgag atgatcggcg agcaggatat cgagccgctg ttgcaagcag
     3721 caaacagcga cagcgaagat gcccagagcg cccgtcagga actggtttcg atgctgatgg
     3781 atcgccacgg caccagccgc gtgctgttcc gtaacacgcg taacggtgtg aaaggcttcc
     3841 cgaaacgcga gctgcatact attaagctgc cactgccgac gcagtatcag accgctatta
     3901 aagtctccgg cattatgggc gcacgtaaaa gtgcggaaga tcgcgctcgc gatatgctct
     3961 acccggagcg tatttatcag gaatttgaag gcgataacgc cacctggtgg aacttcgatc
     4021 cgcgcgttga atggctgatg ggctacctga ccagccatcg ctctcagaaa gtgctggtga
     4081 tctgcgccaa agctgccact gcgctgcaac tggagcaagt actgcgcgaa cgtgaaggta
     4141 tccgcgctgc tgtgttccac gaaggcatgt cgattatcga acgtgaccgc gctgccgcct
     4201 ggtttgctga agaagacacc ggtgcgcagg tactgctgtg ctcggaaatc ggttctgaag
     4261 gacgtaactt ccagttcgcc agccacatgg tgatgtttga cctgccattc aacccggatc
     4321 tgctggagca gcgtattggt cgtctggatc gtatcggtca ggcgcacgat attcagatcc
     4381 atgtgcctta tctggaaaaa actgctcagt cggtgctggt gcgctggtat cacgaaggtc
     4441 tggatgcatt cgagcacacc tgcccgaccg gacgcactat ttacgatagt gtatacaacg
     4501 atctgattaa ctatctggct tcaccggatc aaaccgaagg ctttgacgat ctgatcaaaa
     4561 actgccgcga gcaacatgaa gcgctgaaag cacagctgga acaaggtcgt gaccgcctgc
     4621 tggaaatcca ttccaacggt ggcgaaaaag cccaggcact ggcagaaagc attgaagagc
     4681 aggatgacga taccaacctg atcgccttcg ccatgaacct gttcgatatt atcggtatca
     4741 atcaggacga tcgcggcgac aacatgatcg tgctgacgcc gtccgatcat atgctggtgc
     4801 cggacttccc tggcctgtcg gaagatggca tcaccatcac cttcgaccgt gaagtggcgc
     4861 tggcgcgtga agatgcacag tttattacct gggagcatcc gctgatccgc aacggtctgg
     4921 atctgatcct ttctggcgat accggtagca gcacgatttc actgttgaaa aacaaagcgt
     4981 tgccggtagg tacgctgttg gtggaactga tttatgtggt tgaagcccag gctccgaagc
     5041 agttgcagct caaccgcttc ctgccaccga cgccggtacg tatgctgctg gataaaaacg
     5101 gcaacaacct ggcggcgcag gtagagtttg aaacctttaa ccgccagctt aacgcggtta
     5161 accgtcacac cggcagcaag ctggttaacg ccgtgcagca ggatgttcac gctatccttc
     5221 agctggggga agcgcagatc gagaaatctg cccgtgcatt gattgatgcc gcacgtaacg
     5281 aagccgacga aaaactgtct gccgagctgt ctcgtctgga agcgctgcgt gcagttaacc
     5341 cgaacattcg tgacgacgaa ctgaccgcca ttgagagcaa ccgtcagcag gtaatggaaa
     5401 gcctggatca ggcaggctgg cgtctggatg ccctgcgttt gatcgttgtg acgcatcagt
     5461 aacggagccg aagatgggga tggaaaacta caatccgccg caggaaccct ggctggttgt
     5521 cctgtatcag gatgaccata ttatggtggt caacaagccg agcggtttgt tgtctgtgcc
     5581 gggtcgtctg gaagagcaca aagacagcgt gatgacgcgc attcagcgtg attatccgca
     5641 ggcagaatcg gtgcatcgtc tggatatggc taccagcggc gtgattgtag tggcgctgac
     5701 caaagccgcg gagcgggagt taaaacgcca gttccgcgag cgcgagccta aaaaacagta
     5761 tgtggcccgc gtctgggggc atccatcccc tgcggaaggt ctggtggatc tgccgctgat
     5821 ttgcgactgg ccaaaccgcc cgaaacagaa agtctgttac gaaacaggta aacctgcgca
     5881 gacggagtat gaagtggtgg aatatgcggc ggataacacg gcaagagtgg tgttaaaacc
     5941 gattaccggg cgttcgcatc agctgcgtgt gcatatgctg gcgctgggtc atccgattct
     6001 cggcgatcgt ttttatgcat caccagaagc gagagcaatg gcaccacgtt tgttgctaca
     6061 tgcagagatg ctgacgatta cccatccggc gtatggcaat agtatgacgt ttaaagcacc
     6121 agcggatttt taatagtcac gctggcgaag ttcggtaggc cggagaaggc gttcacgccg
     6181 catccggcaa ttttgcgccg attgcctgat gcgacgcggg ccgcgtctta tcaggcctac
     6241 acgttgaaac ttatttaaac cctttctgca gcttaatcaa ttcataggcc tgctgaattt
     6301 cctgcgcttt ttgcttcgcc atctccatca tctcaggcgg caaacctttc gccaccagct
     6361 tatcgggatg gtgttcactc atcagcttac ggtaggcacg tttgatggtg gtcgcatcat
     6421 ccgtcggctt cacgcccagc acattacagg catcttccag cgttgggcca cgctgcgctt
     6481 gctgccagtt accaccgcca gattgctgct gataaccgcc gccaaactgt gcaccgccct
     6541 gcatcatgcg caaaaactgg tcaaactggg cgcgggagat ccctaattct tcagcaatga
     6601 catacagcac agcccgttca ttcgggtgca gtgaaccatc agcaaacgcc gcctgaatct
     6661 ggatctccag aaacatacga attaagtcaa aacgaccaaa gcagacactg cgaaactggc
     6721 gcatcttttc gcgcagcggg taattgtctg attttcccac ccggaacgcg ttttgcgccg
     6781 cagtacggga agcgccatga aggttcattc ggtccatcaa ctggctggcg atatgaatat
     6841 cagcctccgt gacgcgacct ttggatttgg ttaaatgccc catcacttca aaagtggtgg
     6901 caaaaaacag cgcctgacgc tcacgctggt tggcgaacca cgccatttta cggctacggg
     6961 ctttatcaaa catatggcca attaacaggc ctaacactac gccccaaaag ccgccgccca
     7021 tcagtaaggc cacggccacg ccaatgattt ttccccaata ctgcatatat tccccaaatc
     7081 gacacacgga tatcagggct atctcccaca atataaaggt gcttttaccg ttttccggct
     7141 gcggtcaatt gtgggacatc gcctataatt tgcattatca tacctgtcat tcaatgccgt
     7201 gcctaacacc actgacgcta ttcggacagg attaacacta gcgtagagat gacgagtacg
     7261 ttagtctctg accgtttgtc acgcgcaacg ttaccgatga tggaacaata aaatcaacgt
     7321 atgaaaaaac gtatccccac tctcctggcc accatgattg ccaccgccct ttatagtcaa
     7381 cagggactgg cagccgacct cgcctcacag tgcatgttgg gcgtgccaag ctatgaccgt
     7441 cctctggtac agggcgatac caatgactta cccgtgacta tcaatgctga ccacgcgaaa
     7501 ggggactacc cggatgacgc cgtgtttact ggcagcgtgg atatcatgca gggtaacagc
     7561 cgtctgcagg ccgacgaagt gcagctccat caaaaagagg cgccaggaca accagagccg
     7621 gtacgtaccg ttgatgcgct cggtaatgtc cattacgacg ataaccaggt gatcctcaaa
     7681 gggccgaaag gctgggcgaa tctgaacacc aaagatacca acgtctggga gggtgattac
     7741 cagatggtgg gtcgccaggg tcgcggtaaa gcggacctga tgaaacaacg tggcgaaaac
     7801 cgctatacca ttctggataa cggtagcttt acctcctgtc tgccgggttc tgacacctgg
     7861 agcgtggtag gcagcgaaat tattcatgac cgcgaagaac aagttgcgga gatctggaac
     7921 gcccgcttta aggtgggtcc ggtaccgatc ttttatagcc cctatttgca gttgccggtg
     7981 ggtgacaaac gtcgctctgg tttcttgatc ccgaacgcca agtacaccac caccaactac
     8041 tttgagttct acctgccata ttactggaac atcgcgccaa atatggatgc caccatcacg
     8101 ccgcattata tgcatcgtcg tggcaatatc atgtgggaga acgaattccg ctacctctcc
     8161 caggcgggcg ctggcttgat ggaactggac tatctgcctt cagataaagt ctatgaagat
     8221 gaacacccga acgatgacag ttcacgtcgt tggttattct actggaacca ctccggggtc
     8281 atggatcagg tgtggcgttt caacgtcgac tacaccaagg tcagcgatcc tagctacttc
     8341 aatgattttg ataacaagta cggttccagt actgacggct acgcaacgca aaaattcagc
     8401 gttggctatg cggtgcaaaa cttcaatgcc accgtttcaa ccaagcagtt ccaggtcttc
     8461 agcgagcaga acaccagtag ctactcggca gagccgcagt tagacgttaa ctactaccag
     8521 aatgatgttg gtccgtttga tacacgcatt tacggccagg cagtgcactt tgttaacacc
     8581 agagacgaca tgcctgaagc aacccgtgtt cacctggaac cgaccatcaa tttgccgctc
     8641 tctaataact ggggcagcat caataccgaa gcgaagttgc tggcaaccca ttatcagcaa
     8701 actaatcttg actggtataa ctccagaaac acaaccaagc tggacgaatc cgttaaccgc
     8761 gtaatgccgc aattcaaagt tgacggcaaa atggtctttg aacgcgatat ggaaatgctg
     8821 gcgccgggtt atacccaaac gctggaaccg cgcgcgcagt atttatacgt gccgtatcgc
     8881 gatcagagcg acatctataa ctacgactcg tctctgctgc aatctgacta ctctggcctg
     8941 ttccgggacc ggacttacgg cggtcttgac cgtattgcct ccgctaacca ggtgacgacc
     9001 ggtgtcacat ctcgcatata tgatgatgct gccgttgaac gttttaatat ttccgttggt
     9061 caaatctact atttcacgga gtctcgcact ggcgatgaca acataacatg ggagaatgac
     9121 gacaaaacgg gctcactggt gtgggcaggc gatacttact ggcgtatctc agagcgttgg
     9181 ggattgcgtg gcggaattca gtacgataca cgtctggata acgtagcgac cagtaactcc
     9241 agcattgaat accgtcggga tgaagaccgt ctggtacagc tgaattaccg ttacgccagc
     9301 ccggaatata ttcaggctac gctgcctaag tactattcca ctgctgagca atataagaat
     9361 ggtatttcgc aggtaggtgc cgtcgccagc tggccaattg ccgatcgttg gtcaattgtt
     9421 ggggcctact actacgacac caatgcgaac aagcaagccg actctatgtt aggtgtgcaa
     9481 tacagctcct gctgctatgc aattcgcgtc ggttacgagc ggaagctgaa cggttgggat
     9541 aacgataaac aacatgcggt atatgacaac gcaatcggct ttaacatcga acttcgcggc
     9601 ctgagctcca actacggtct gggtacgcaa gagatgctgc gttcgaacat tctgccgtat
     9661 caaaacactt tgtgatttgt tgatttacca cgtaatccgc agtgcggtta attgaaatgg
     9721 aaaaagtatg aagaactgga aaacgctgct tctcggtatc gccatgatcg cgaataccag
     9781 tttcgctgcc ccccaggtag tcgataaagt cgcagccgtc gtcaataacg gcgtcgtgct
     9841 ggaaagcgac gttgatggat taatgcaatc ggtaaaactg aacgctgctc aggcaaggca
     9901 gcagcttcct gatgacgcga cgctgcgcca ccaaatcatg gaacgtttga tcatggatca
     9961 aatcatcctg cagatggggc agaaaatggg agtgaaaatc tccgatgagc agctggatca
    10021 ggcgattgct aacatcgcga aacagaacaa catgacgctg gatcagatgc gcagccgtct
    10081 ggcttacgat ggtctgaact acaacaccta tcgtaaccag atccgcaaag agatgattat
    10141 ctctgaagtg cgtaacaacg aggtgcgtcg tcgcatcacc atcctgccgc aggaagtcga
    10201 atccctggcg cagcaggtgg gtaaccaaaa cgacgccagc actgagctga acctgagcca
    10261 catcctgatc ccgctgccgg aaaacccgac ctctgatcaa gtgaacgaag cggaaagcca
    10321 ggcgcgtgcc attgtcgatc aggcgcgtaa cggcgctgat ttcggtaagc tggcgattgc
    10381 tcattctgcc gaccagcagg cgctgaacgg cggccagatg ggctggggcc gtattcagga
    10441 gttgccgggg atcttcgccc aggcattaag caccgcgaag aaaggcgaca ttgttggccc
    10501 gattcgttcc ggcgttggct tccatattct gaaagttaac gacctgcgcg gcgaaagcaa
    10561 aaatatctcg gtgaccgaag ttcatgctcg ccatattctg ctgaaaccgt cgccgatcat
    10621 gactgacgaa caggcccgcg tgaaactgga acagattgct gccgatatca agagtggtaa
    10681 aacgactttt gctgccgcag cgaaagagtt ctctcaggat ccaggctctg ctaaccaggg
    10741 cggtgatctc ggttgggcta caccagatat tttcgatccg gccttccgtg acgccctgac
    10801 ccgcctgaac aaaggtcaaa tgagtgcacc ggttcactct tcattcggct ggcatttaat
    10861 cgaactgctg gatacccgta atgtcgataa aaccgacgct gcgcagaaag atcgtgcata
    10921 ccgcatgctg atgaaccgta agttctcgga agaagcagca agctggatgc aggaacaacg
    10981 tgccagcgcc tacgttaaaa tcctgagcaa ctaatggtta aaacccaacg tgttgtgatc
    11041 actcccggcg agcccgccgg gattggcccg gacttagttg tccagcttgc acagcgtgag
    11101 tggccggtcg aactggttgt ttgtgccgat gccactctcc ttaccgaccg ggcagcgatg
    11161 ctcggtttgc cgctcaccct ccgtccttat tcccccaact cccctgcaca accgcaaact
    11221 gcgggcacat taacgctact tcctgtcgcg ctacgtgaat ctgtcactgc ggggcagtta
    11281 gcggttgaaa atgggcatta cgtagtggag acgctggcgc gagcgtgtga tggctgtctg
    11341 aacggtgaat ttgctgcgct gatcacaggc cccgtgcata aaggcgtcat taacgacgca
    11401 ggcattccgt ttaccggtca taccgagttt ttcgaagagc gttcgcaggc gaaaaaagtg
    11461 gtgatgatgc tggcgaccga agaacttcgc gtggcgctgg caacgacgca tttaccgctg
    11521 cgcgatatcg cagatgctat cacccctgcg cttttgcacg aagtgattgc tattttgcat
    11581 cacgatttgc ggaccaaatt tggtattgcc gaaccgcgca ttctggtctg cgggctgaat
    11641 ccgcacgcgg gcgaaggcgg tcatatgggt acggaagaga tagacaccat tattccggtg
    11701 ctcgacaagc tgcgggcgca ggggatgaaa ctcaacgggc cgctgcctgc cgataccctg
    11761 tttcagccga aatatcttga taacgccgac gcggtgctgg cgatgtacca cgatcagggt
    11821 cttcccgtgc taaaatacca gggcttcggg cgcggtgtga acattacgct gggcctgccc
    11881 tttattcgca catcagtgga ccacggtacc gcgcttgaac tggcgggacg tggcaaagcc
    11941 gatgtcggca gttttattac ggcgcttaat ctcgccatca aaatgattgt taacacccaa
    12001 tgaataatcg agtccaccag ggccacttag cccgtaaacg cttcgggcaa aactttctca
    12061 acgatcagtt cgtgatcgac agcattgttt ctgccattaa cccgcagaag ggccaggcga
    12121 tggtcgaaat cggccccggt ctggcggcat tgaccgaacc ggtcggcgaa cgtctggata
    12181 agctcacggt catcgaactt gaccgcgatc tggcggcacg tctgcaaacg catccattct
    12241 taggcccgaa actgactatt tatcagcagg atgcgatgac ctttaacttt ggtgaactgg
    12301 ccgagaaaat gggtcagccg ctgcgtgttt tcggcaacct gccttataac atctccacgc
    12361 cgttgatgtt ccatctgttt agctatactg atgccattgc cgacatgcac tttatgttgc
    12421 aaaaagaggt ggtgaatcgt ctggttgcag gaccgaacag caaggcgtat ggtcgattaa
    12481 gcgtcatggc gcaatactat tgcaacgtga tcccggtgct ggaagtaccg ccgtcagcct
    12541 ttacaccacc acccaaagtg gattccgccg tcgtgcgcct ggttcctcat gcaacgatgc
    12601 ctcacccggt taaagatgtt cgcgtgttga gccgcatcac caccgaagcc tttaaccagc
    12661 gtcgtaaaac cattcgtaac agcctcggca acctgtttag cgtcgaggtg ttaacgggaa
    12721 tggggatcga cccggcgatg cgagcggaaa atatctctgt cgcgcaatat tgccagatgg
    12781 cgaactatct ggcggagaac gcgcctttgc aggagagtta acgatgatca attcgccccg
    12841 agtgtgtatt caggttcaaa gcgtctacat tgaggcacaa tcttcacctg ataatgaacg
    12901 ttacgttttt gcttataccg taaccatacg caatctgggg cgagcgccag tgcagttgtt
    12961 ggggcgttac tggctgatca ccaatggcaa tggccgtgaa accgaagtcc agggcgaagg
    13021 agtggttggc gtccagccac ttatcgcacc tggcgaagag taccagtaca ccagcggtgc
    13081 aatcattgaa accccgctgg gcaccatgca gggtcactac gaaatgatcg atgaaaatgg
    13141 cgtccctttc agcatcgaca ttcccgtatt ccgactcgcc gttcccacac tcattcatta
    13201 aaagaatatg gcgacatacc ttattggcga cgttcatggt tgttacgatg aactgatcgc
    13261 attgctgcat aaagtagaat ttacccctgg gaaagatacc ctctggctga cgggcgatct
    13321 ggtcgcgcgc ggcccgggtt cgctggatgt tctgcgctat gtgaaatcct taggcgacag
    13381 cgtacgtctg gtgctgggca atcacgatct gcatctgctg gcggtatttg ccgggatcag
    13441 ccgcaataaa ccgaaagatc gcctgacacc gctgctggaa gcgccggatg ccgacgagct
    13501 gcttaactgg ctgcgtcgcc agcctctgct gcaaatagac gaagagaaaa agctggtgat
    13561 ggcccacgcc gggatcacgc cacagtggga tctacagacc gccaaagagt gcgcgcgcga
    13621 tgtagaagcg gtgctgtcga gcgactccta ccccttcttc cttgatgcca tgtacggcga
    13681 tatgccaaat aactggtcac cggaattgcg ggggctggga agactgcggt ttatcaccaa
    13741 cgcctttacc cgtatgcgtt tttgcttccc gaacggtcaa ctggatatgt acagcaaaga
    13801 atcgccggaa gaggcccctg ccccactgaa accgtggttt gcgattcctg gccctgtcgc
    13861 tgaagagtac agcatcgcct ttggtcactg ggcatcgctg gaaggcaaag gtacgccgga
    13921 aggtatttac gcgctggata ctggctgctg ctggggtggt acattaacct gcctgcgctg
    13981 ggaagataaa cactattttg tccagccgtc gaaccggcat aaggatttgg gcgaagcggc
    14041 ggcgtcttaa acacagcctg atacaggaag gccggataag acgcgaccgg cgtcgcatcc
    14101 ggcactagcc gtaaattcta tacaaactta ccgccgctcc agaatctcaa agcaatagct
    14161 gtgagagttc tgcgcatcag cgtcgtggaa ttcgctgaat accgattccc agtcatccgg
    14221 ctcgtaatcc gggaaatggg tgtcgccttc cacttctgcg tcgatatgcg tcagatacag
    14281 tttttgcgct ttgggcagga actgctcata aacgcgaccg ccgccaatca ccatgatttc
    14341 tggtacgtca ccacacgccg caatggcttc atccaccgac ttcacccacg ttacgcgatc
    14401 gtccgtaccc ggttgactgc tgaggataat atttttgcgt cctggcaacg gacgaccgat
    14461 ggattcccag gtatggcggc ccataatcac gggtttattt aaagtgttgc gtttaaacca
    14521 ggcgagatcg gcaggcaggt tccacggcat ggcgttttcc atgccgataa cacgatctac
    14581 cgctaacgcc gcaatcagac tgatcattga gatttcccga taaaaaaaat tgtcgccact
    14641 atacgtaaag cgtaaaccgt cgtcgactgg tgcgaggatg atgttgagga aaattttata
    14701 ttctgctggc gagtccacgc tctcccactg gactcgccgc attacaatga aataaaaaca
    14761 attagttagc tgtaaagtgt gatttacgtc actctttatt aggatgaggg tttcgtttcc
    14821 ggttcatccg ccatgttgcc ggtatgttta ccttcttcgg ttccctgcca gccatgtcgt
    14881 tgaattaatg acagatgttc gcggtcctcg gtaatgatct cacttaacat cgcgctggtg
    14941 cgtttataga ccgccgcgcg ggctttggtg tcgttctcaa ccattgccat ctcttccacc
    15001 atctgaatat taaagcggcg gaacacatcg gcgcgttctc gcgcttcata cggccccaga
    15061 cctaaacttt ccagtgccag acgcccggtt ttcagcgcac cttcaaaggt ttcacgctcc
    15121 ggcttttcaa cgcctgcctg acgcaaacga atgtagtggt cgacatcgcg ggcgcgggca
    15181 ataatctgca aatgcgggaa atgttctttc accatctccg tcagttgcag gttggtttgc
    15241 ggatcgtcga tagcgttaat cagcacttcc gctttcgccg ctccggcaga ttccagtaaa
    15301 tccatccgcg tggcatcgcc ataaaacact ttcataccaa atttacgcaa ggtttcgata
    15361 tggtccggat cgtgatcgag caccaccatt ttcaccccgc tggagagcag taaacgtccg
    15421 gtgatctgcc caaaacgacc gaatccggcg atgattaccc gcggctgttc ttcgtcgatc
    15481 tcatcggctt cacgcgcctc ttcactagaa gattgctcaa ggcgattgag gatcaccagc
    15541 agaatcggcg ttgccgccat cgacagcgcc accgccaggg tcagcgattt cgcccactcc
    15601 ggctccagca cattcgccat ctgcgccgca ccaaatacta caaaggcaaa ctcactaccc
    15661 tgccctaata gcaccgcaaa ccagcgacgc tgtttatttg gcacctgcaa cggccgggca
    15721 atcagccaca gcatggcgat tttgatgatg aggaaaccga gcagcaaaat gacaatgcgc
    15781 aacggatttt caagcagcgt gccaaagtct atcgacatgc caacgccgat gaaaaacagc
    15841 cccagcagca aacctttaaa tggttcgata tcgctttcca gcgcatgacg gtattccgag
    15901 cttgccagca gtacgcccgc cagaaacgcg cccatcgcca tcgacaagcc gacctcttcc
    15961 agcagcaaac caaagccaaa cacgaggaat aacgccacgg cactaaacac ttcccgcaag
    16021 ccagagcggg caacaaaacg cagtgccgga cgcgtgacat agcgccccag caataccacc
    16081 agcaccagcg cacccgccac ttttaacgcc gagagagcaa atgcgcccat cgtcgtcgag
    16141 gcactgctcg ccgccagtag cgggatcatc gccaccagcg ggatcgccgc gatatcctgg
    16201 aacagcagca ccgcaaaggc actgcgaccc atttgcgtca ccatcagatt gcgttcattc
    16261 atcgcctgca tggcaatcgc cgtagaggag agcgccagcg tcatgccgat caattccgcg
    16321 acctgccagc gcagcccaag caacatacag aacagcccca gcaggccgcc gcaaatcacc
    16381 atctgcaatg cgccgccgcc gaacaccgcc gcacgcagct tccacagcct ttgtggatcg
    16441 agttcgaggc cgataataaa cagcatcagc accacgccaa tctcggcaaa gtgcagaata
    16501 gattcggcat cggtcaccag tcgcagcccc cacgggccaa taatgcagcc ggcgatcagg
    16561 tagccaagta ccgctcccag accgagacgt accgcaatgg gaacaatcag cgccgccgaa
    16621 ccgagataaa tcagcgcctg aataagcgta tggctatcca tgatgtgcct cctgccattc
    16681 cagcagacgt tgcttatagt gccgcgcctg cccttcgagg gtttcgtcgt cacaaataaa
    16741 ggtgcagtgc atggcaaacg gtggcagcca gttcagcccg cagtagattg ccgtcgcctg
    16801 tagcggctgt gacagcacat caaagcccgg atgcgcacca atctcaaaat ggctttcccc
    16861 accgccggtc gtcaccgccc acagtaaatg tttgccatgc agcgccgtgc cgccgtgacc
    16921 gtaagcccag ccgtgcgaga agactttatc gatccaaagt ttaagaagcg gaggaatgct
    16981 gtaccactgc atcgggtgct gccagacgat cagatcggcg cgagacagcg cctcctgctc
    17041 ggcggcaata tcgatattga agtcaggata gagttgataa agagagcgaa tttcgacgcc
    17101 ttccagcgtc cttgcctgtt caagcatccg tttattcgca tgggaatgat gcggatacgg
    17161 atgcgcataa attataagaa tcataggtag cccgcgtgat tacgcctttg ttttcccaca
    17221 aagtgtagtc atatattcac gcggcttata gtcaataata ttgattcaga aaagagagaa
    17281 atctgaacaa attaccccat tctctgcccg cggatcgtca tattgctggt ctgcgccagt
    17341 gacatccctc gagtctccgg ggcaaacgct acggaaatca acaagccaaa cagcgagata
    17401 cccgccccca ttagcatcgt gttactgata ccgtaattat tgataaagat cggcagtgcc
    17461 caggtcgaaa caatagtgcc aatacggctt aaggacataa tcacgcccac ggcagaggcg
    17521 cggatatccg tcgggaagag ttcattagga tagagccact gcaaattacc cggcccgcca
    17581 gagaaaaagg catacaccgc aaatgccatc actaccagcc agatccccat atccgggatc
    17641 agccccaaaa ccgccagcgc cagcgtcatc atggcaaagc tgccaatcaa caatggacgc
    17701 cgtccggcag tgtttagcca cagcatcggt ggaatacagc cgagcataaa gaacaggcta
    17761 atcaccacgt tccccagtgc cgcgtttttg ccaacaccca atcccaacaa accaacgatt
    17821 tgcgggccaa aggtgtagat ggcgaacatc gggatcacct ggcaggtcca gatggcggca
    17881 acaaacagaa caaaaggaaa atggcggcga ttaaacagat cgcgaaaacg ggtttgctgc
    17941 ggctgctctt catcgaaagc caccggttcg ccaaacagtt tgatcatcat ctcttcgcac
    18001 tcttttactc gccctttgcg taacaaccag cggggagatt caggcagttc gaatcgacca
    18061 atcaaaatca acaaacaggg gatcgctgcg ctacccagca tccagcgcca accaccttcc
    18121 acatcataaa gccagtaacc gaccagatcg gcgcaggtcg cgccgacata ccacatcgcg
    18181 gcaataaagc tgatggaaaa cgcccgctga cgggtactgg agaactcagt gatcatcgaa
    18241 gtggcgatgg gataatctgc accgatgaca atgccgatga gtacccgcat cactaacagt
    18301 tcgacggggg atgagacaaa catcgtcgcc accgatatca cgccgatggc gatgatatca
    18361 atgaggaaca ttttgcgccg tccgacttta tcggagatat agccaaacag cgatgtgcca
    18421 acgaacagcc cggcgagcgt tcccgcgccc agcaagccaa tccagtcagc gtccagtttc
    18481 agcgccggag tcagttgctc cagcgccacg ccaatcatta ccagtacata accatccaga
    18541 aacggaccac cgcttcccca cagcaaaatg cggcggtgaa tagaggagaa tttaagatca
    18601 tcaaagtttc tggacggttg catgggttat tcctgttttt ttgcaatgag agtcagtacg
    18661 gcattacgta tcaaaccaac atcagccgta acggaactcc acaccaaagg tgccgcgcgg
    18721 gtattcccac tgttccagcg ccgaccccag ccccagaatg cgacaggtgc cgcactccag
    18781 acatccggcg taatcgaagc gcacactgcc gtcatcctgc ttcttgtaca gacctgcggg
    18841 gcacgctttc accagcagct ccagggcctg tttatcggca tcggccttca caacgatgtg
    18901 cggatgctct tcatcgacat tgaatttatt gacgcccagt ttgacgtcca cattgacggg
    18961 agaagtcata aaacggtcac tcctttcatg ccatccttga tcagattgat gaagcccact
    19021 ttcttgccgt ggcggaggat tttcttgcgc atcagttccg gcgcactgcc gtcaatggtg
    19081 aacaggtcac gcgccacgcc caccgccagt tccgggtagc cgctaaacat gcgtgggtta
    19141 tcaaggaacg ccggtagttt ctggtacatg cgcatatcgc gcagcgggcc actctcaaga
    19201 tgctgacgat attccgcgag tttttgctta ctgaaatcgt cgcttttcat cgctgaaagc
    19261 acagtttttg ctgctgcttc cccggcggca atcgccagat ccataccgcg aatggtaaaa
    19321 ccgaggttca tacacattcc ggcagcatca ccggcaatca atacgccgtc accaaccaac
    19381 tccggcagca tgttgatacc tgcttccggc actacgtgag cggaatattc caccagcttg
    19441 ccgcccgcga tcagcggtgc aacggccgga tgttgtttga aatcttccag catttgcggc
    19501 accgattttt ttgcgtcatg cagatgatgc aaaccacaaa ccagccccag cgacagggtg
    19561 ttttcattgg tataaagaaa gccgccgccc atcaggccat cagtggggga acccgcaaac
    19621 aggcaagccg caccctgatt accctgcaac tgaaaacggt cttcgataac cgacttcggt
    19681 aattcgatca gttccttcac gccaaccgcc acatccgtcg gtttaacgcg ttttgccatc
    19741 cccagctttt cggcaaggat ggagttcacc ccgtcagcaa ggatcaccgt tttcgcttca
    19801 attacatcgc catcggcttc tacgccgaca actttgccat cgcgctgtac gaggttatcg
    19861 acacgaatcc cggtaattaa ctgcgcaccc gcttcttcgg cctgctccat cagccaggca
    19921 tcaaatttac tgcgcaaaac ggagtaagaa cgctgggatg gtgaggtttc gtcaccgttg
    19981 cagtagtcca tagtcatcgc agacttttcc gtcataaacg cgagtttttc atgggtgatc
    20041 aggcgttcta cgggggcgga gtcagcgaaa ccaggaataa tgtgttccag actgtgggca
    20101 tagagacgcc cgccggtgac gttcttggca ccagcggaat tgcctcgctc gataactaac
    20161 acttgcgccc cttcgcgggc gagcaccagt gcggcaaccg aaccggcaag ccctgcaccg
    20221 acgatgatgg cgtcaaagat atcttcggac ataagagctc cctgtcagcg gcagggccaa
    20281 aatgcagccc tgccagagtg gatcaacgcg ctaaagctgc ggtcagtgcc ggaaggatct
    20341 tcacggcgtc gccaacaatg ccgtaatccg cgtactggaa gatcggcgca tttttatctt
    20401 tgttgatggc gaaaatggtt tgcgacgcat tagcgccaac catgtgctgg atctgcccgg
    20461 agatccccac cgccaggtac agttcaggtt tcagcatcag gttggagata ccgacatagc
    20521 gttcgtgctc catccatttt tcgttttccg ccaccggacg agaacaggcc aactccgcac
    20581 ctatcgcctt gcaaagctgt tctgccagcg caatgttctc tttgctgcca ataccgcgac
    20641 caacgctgac caccaggcgg gctttgtcga gatcgacgct gttgctctga cgcgcctggg
    20701 ttgccgtgcg ggtaatcgcc acagccggag cctgccactc cacggtgtgc gtttcgccag
    20761 tgcgtgacgc gtctggctga gccacatcga acgtgccgct gctgatggtc agaaccgcat
    20821 acggcgtggc aatgcgttct tcgccaatcg ccagaccgcc gtaaaccatg tgtttcactg
    20881 tcgctttacc gtcctgcacg ctaacagcgc tggcatcgtt agacaccgcc gcgttaaggc
    20941 ggtaacccag tttcgccgcc agtaatttgc cgcgacgggt gtttggcaac agcaccagac
    21001 cgtctgcgcc gtgctggcga atagtgtcag ccatgacacc ggcgtaatct tcgatcatcc
    21061 gctcgtccgg tttgccgctt aatttccaga catgattagc gccgagctgg attgcctgtg
    21121 cgccgtcggc atcattgagg acgaacgtgt tgatttgatt agctaaagcc tgcgcaccgt
    21181 tcatcagttc cggcagacga gaaggggtat cgctgaatac ccagacttga gaaaacgtgt
    21241 tcatagcatc ccctgtaatt aaatgacttt gcgaagattt tcagcaaatg cggcgatctg
    21301 ttcttcgccg tcgccttcaa tcacgatgcg ctgacgctcg cgctgtttcg gcgcggcaac
    21361 ctgttgttct gaccaggctg cctctgcgtt aaaaccgata tccgccgccg accatacctg
    21421 gactggcttt ttcgccgcgc cgagaatggc tttcatcgaa ggaatttgtg gggagttgat
    21481 atcagtggaa acagcaacaa ccgcaggcag cggaatgctt aaggtttctg tttcatcttc
    21541 cagttcgcgc tcaacggtga gggtatctgc agtcagggag ataattttgc tgacgccgtt
    21601 aactgccgga atattaagga tttcgcctac cagcaaacca acctgctggg cataaaggtc
    21661 ggaagaacca tcgccacaga ggatcaaatc aaagcctgct ttctgagcgg ctgcagccag
    21721 tacgctcgcc gtttgttgcg gcagtgcctg ctcgaactgg tcatcaatca ccacaatcag
    21781 ttcatccgga ccgcgcgata gcacatcttt acgccctttg gcgttggtca gggctttacc
    21841 gcccacactt aaggccgtca cctgcgcctc tgctgcctgt tgctttagct ggcaagccgc
    21901 ttcaatagcg ttgagatcgt attggcttat tttggcatcg gctttgctga agtctaacga
    21961 accatcagca ttattgaccg caatatcctg ttcatcaggc acgcacttat agcaagtaat
    22021 aatcttcatt gcatctccag aaatcatgaa ggaaacgtcg ttcatatcgg tggtgttaat
    22081 tacagatatc acgcccttta atttggaaat aaaactttaa tcaccaatat tgaaaatgtc
    22141 acacgcagat aaatccaac
//
LOCUS       DAZKGF010000079        21930 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZKGF010000079 DAZKGF010000000
VERSION     DAZKGF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.079"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..3649)
                     /locus_tag="ACHOQV_002787"
     CDS             complement(<1..3649)
                     /locus_tag="ACHOQV_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFP9631135.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVKINGKPAARAAVTLPPP
                     PLTEAIPSPPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGIMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLYFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTNDDRLAHSPGDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVNVTGADGYYNATLDYSDGCTTVTDGKGIHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAVHGQLSRYTAADGAVWL
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERAGTGAEGFRYDRHGNLLAWTDGNGVDWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYLLDNCGRVTEEHDWGGVTWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEIITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEEATWGGLVPSLVREYRYDSA
                     GNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQ
                     SAGRHRISEETE"
     gene            complement(3669..4130)
                     /locus_tag="ACHOQV_002788"
     CDS             complement(3669..4130)
                     /locus_tag="ACHOQV_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFP9631136.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRIDQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(4158..6059)
                     /locus_tag="ACHOQV_002789"
     CDS             complement(4158..6059)
                     /locus_tag="ACHOQV_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP9631137.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVRPPLWRA
                     GLRQNFRIFQNEDIRSILGTILQENDVTEWSPLFSEPHPSREFCVQYGETDYDFLCRI
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDWPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTEHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPKPKPSVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPANQDSSCWIRVAQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HEDNRSPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGKEQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVMIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKQIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            complement(6803..8251)
                     /gene="cusS"
                     /locus_tag="ACHOQV_002790"
     CDS             complement(6803..8251)
                     /gene="cusS"
                     /locus_tag="ACHOQV_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFP9631138.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQAQRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDTRGGEVYLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFMIKLPEKG"
     gene            complement(8241..8924)
                     /gene="cusR"
                     /locus_tag="ACHOQV_002791"
     CDS             complement(8241..8924)
                     /gene="cusR"
                     /locus_tag="ACHOQV_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFP9631139.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            9081..10463
                     /gene="cusC"
                     /locus_tag="ACHOQV_002792"
     CDS             9081..10463
                     /gene="cusC"
                     /locus_tag="ACHOQV_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFP9631140.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLNQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSNNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            10487..10819
                     /gene="cusF"
                     /locus_tag="ACHOQV_002793"
     CDS             10487..10819
                     /gene="cusF"
                     /locus_tag="ACHOQV_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFP9631141.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KTIDLESKKIIIHHDPITAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            10835..12058
                     /gene="cusB"
                     /locus_tag="ACHOQV_002794"
     CDS             10835..12058
                     /gene="cusB"
                     /locus_tag="ACHOQV_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFP9631142.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWITAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADESLKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            12070..15213
                     /gene="cusA"
                     /locus_tag="ACHOQV_002795"
     CDS             12070..15213
                     /gene="cusA"
                     /locus_tag="ACHOQV_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFP9631143.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGQTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            15315..16691
                     /gene="pheP"
                     /locus_tag="ACHOQV_002796"
     CDS             15315..16691
                     /gene="pheP"
                     /locus_tag="ACHOQV_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFP9631144.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(16772..18019)
                     /gene="ybdG"
                     /locus_tag="ACHOQV_002797"
     CDS             complement(16772..18019)
                     /gene="ybdG"
                     /locus_tag="ACHOQV_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFP9631145.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(18127..18780)
                     /gene="nfsB"
                     /locus_tag="ACHOQV_002798"
     CDS             complement(18127..18780)
                     /gene="nfsB"
                     /locus_tag="ACHOQV_002798"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFP9631146.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQVKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLEEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            complement(18874..19242)
                     /gene="ybdF"
                     /locus_tag="ACHOQV_002799"
     CDS             complement(18874..19242)
                     /gene="ybdF"
                     /locus_tag="ACHOQV_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9631147.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            complement(19307..19555)
                     /gene="ybdJ"
                     /locus_tag="ACHOQV_002800"
     CDS             complement(19307..19555)
                     /gene="ybdJ"
                     /locus_tag="ACHOQV_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFP9631148.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            complement(19621..20739)
                     /gene="ybdK"
                     /locus_tag="ACHOQV_002801"
     CDS             complement(19621..20739)
                     /gene="ybdK"
                     /locus_tag="ACHOQV_002801"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFP9631149.1"
                     /translation="MPIPDFQVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLMEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            complement(20944..21212)
                     /locus_tag="ACHOQV_002802"
                     /pseudo
     CDS             complement(20944..21212)
                     /locus_tag="ACHOQV_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21256..21701)
                     /locus_tag="ACHOQV_002803"
                     /pseudo
     CDS             complement(21256..21701)
                     /locus_tag="ACHOQV_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
BASE COUNT         5016 a         5704 c         5891 g         5319 t
ORIGIN      
        1 gctcagtctc ttcacttatc cggtgcctgc ccgcagactg cgccttcagg tagccgcagc
       61 tgtcatagcc ataagtctca tcgccttcgc catagcccag cccggtgcct gaggctgtca
      121 ccgccgtgac ctggccgttc cggtccagcc ggtactcccg gcgtgtttcc cgtccgtaat
      181 cttcccggct ggtgacacct gacacgttac ccgcgctgtc gtaacggtac tcccgtacca
      241 gtgacggaac cagcccgccc catgtggctt cttccggaaa aaatccggcg cgctggctga
      301 caggctggcc ggctgcattc cagccctgct cacaggcaaa accccggtta ctgtgccgga
      361 gcacttcacg tcctgcctca tcacggctga acgtcagcgt ctcatccccc ggcaggataa
      421 cctccgtcag ttctccggcg gcgttgtagc ggtaggtggt tttcagcagg gattcatcgg
      481 ggtgtctgtc gcgttcatct tccggtggca ggatacagcg gatgacggtg tgttcatcgg
      541 ggtaatggta ctcaatggcc cgtcggccct gagtggtgac attcacccgc ccgcgttcgt
      601 catagccggt gcgctgtgat gtgccgtccg ggctgaagat acccgtcagc ctgccggatt
      661 tgtcatacgc atactgcgtt ttgccttccg gcgtgatgat ttctgccagg cggcctgcgg
      721 cgtcccggct gtagaggatg gtttcctcca ggccgttaac cctggcggta cacaggccat
      781 cggcgtcata gcggtaacgc caggtcacgc cgccccagtc atgctcttcc gtcacccgcc
      841 cacagttgtc gagaagataa cggtatgact caccctgcgg gttgatgact tctgtcagtt
      901 gcagcgtgtc tttatcatag cggtactgcc agcggtggcc ttcaccgtcc gttcgcgcca
      961 ccggcaaatc aaacgggccg tattccattg tccagtccac accgttaccg tccgtccagg
     1021 ccagcaggtt gccgtggcgg tcatagcgga atccttctgc gccggttcct gcccgctcaa
     1081 ggccgctcag tttatggtgc tgctgccact gcaaccgcac tgactgaccg ccggggccgc
     1141 gcatcttcac cggcaggtcg tgaccactgt attcggtgtg gtgttcacgg cccatcacgt
     1201 cggtgagtgt ttccggccgg ttgtggtggt cgtactctat gcccagacgc cgttcatcct
     1261 ggcggaatat ccccgacagc agcccctgag cggtgtacgc cagccgggtc tcttcgccct
     1321 gtggcgatac cagactcacc ggcaggccgc gttcatcgca ctgctgtgtc cacgtctgcc
     1381 cggcagggtc ggtgatgttg ctgagcaggc cgcgctcatc atagcggtac aaccacaccg
     1441 cgccgtctgc cgccgtataa cggcttaact gaccgtgtac ggcgttgtat tcaaatttct
     1501 ccacccgtcc ggcaggggag tggcgggcca gcaggtgatg gaattcatcc cattcatacg
     1561 tggtggtgct gccgtccggc gcttcttccc gcagaatatt gccgtcagga tcatagtaat
     1621 aacggtgaat gcccttgccg tccgtcacgg tggtgcagcc gtcactatag tccagcgtgg
     1681 cgttgtaata gccgtccgcc ccggtgacat tcacgcaccg gccctgttca tcgtaggtga
     1741 aacggctcca cgtctggtcg ttatcggacc agcggatgat ccggtccgca gcgtcgtact
     1801 cataaaacag gtggtagtcc agccgcgcat ccgcttcggt cagccgcccc cgcgcatcct
     1861 gttcgtaggt cgccagcgtc tgtatgccgt tatccgtgcg gtgaatggct ttcaggtagc
     1921 cgctggcgtg gtacgtcagt ttaagccgga tcccgtcggt gtgactgact cccgtgatgc
     1981 ggtactggct gtcgcgctca aacgtgatgc ggttgctggc ggtgtcgctc atcccgccca
     2041 gcatgtacat ccggtcaccg gggctgtgtg ccaggcggtc atcattggta tcgtcagtca
     2101 ccgggtggcg caggcgcatt cccggaaagg cgtcataaaa gaagcggctg ctcagggcat
     2161 cacggtcgaa cagctccagg taatcgtcgc ggcggcgcag gatatactgg gcgtgatacg
     2221 ggttaaccgc cgtatcactg tctccgtcga aggcaaagta cagcgtggct ccctggctga
     2281 tgatatccac ccggtttccc tgttcacgca cccaggcgta ttcgctccag gagtcgctcc
     2341 agcccacgcc cgtcaggcca tgcagtggca gtacagaact gtggaaacgg gtgaaggaga
     2401 gggggagtgt ctggcccagc gtaaagtcgg tacgttcgtc gcagtatgcg ccggttaccg
     2461 gatcgacagg atctgttacc catctgatga ctttattggc aatacttttt aacgacttac
     2521 ctgttttccc cagcaacgcg ccgagctttg cccccagcag cttcccgctt ttccccagca
     2581 ggtttcccag tttgccgagt ttcgccagat tccccagttt tcccagcagt cccgcgggcg
     2641 gcagaagata gctggctatc gttattcccc ccaggatcat tctctgccac ggcgggaact
     2701 ccggctggat atccagatag gtcacctgtt caccgccaat aaagacgtcc ggcgaacctt
     2761 ccacgattgc cgcgctgcac tccagtttgt cgcccttgcg cgcggcaggc tgattgttga
     2821 taaagacgtt gctgctgccc tgggctatcc tcggtggttt gttctccttg tcgcagagcg
     2881 ccgtgtcctc accggcttcc gggaaatcca cgtctttttt gatttcgcca cgcccgccca
     2941 ccatggtggc aaactccatt aataccggac cggcgggaaa cagaagcgat gcgccggcgc
     3001 tgacgcgatc cataataccg gcattgctgt ccgtcagggt gataacagcg ttcccccagg
     3061 ccttcgccag tggcactgct ttttccgctg ccgattcaag caggtcagtg gcaatatccc
     3121 cccatgaagg ttccccctgt ggtggagagg gtattgcttc agtcagggga ggagggggaa
     3181 gggtgacggc ggccctggct gctggcttac cgttaatttt tacgttttca gaaccggtat
     3241 tgatggcgcc gtccgtgtca caaaaactgt ttatccatga ctcaagctgg ttctgtttct
     3301 cctccacata atcactcgcc ttatagctgg catacatcag cgcaggcacc agaaccagcg
     3361 cataagggcc ggtggcgcag gcggccataa tcaggcaacc ttctgcgata gttactatcg
     3421 cccccagcac ggcccctgcc agtgccccca gaaaactttt atgctgaatt tcatcgccca
     3481 gcctggcggc aggtgggccg gacttctttg ctgccagcat ctgtgcaatg gctttgtcgt
     3541 tggctgcccg tttcatctgg ctggcgacca tcgcctgctg tgacagcatt cgcattgcca
     3601 gcgtaccgcc tgcggtcgct ccctgtgggc cgcctggtcc ttcactcatg tgttatccct
     3661 cgtttatgtc agcgcggctg gtccttttgg atgtcattct ccggtggcaa cggggtgaag
     3721 ctctttatca cctccagcag catcgtcttt tgactgttct tcagggataa ggaggtgctg
     3781 aaagtgaagg tcagcagcgt atctccacgc cgctggaata cggctatctg gtcaatccgt
     3841 ccctggttcc cctcatcttc cggaccgttc caggaataat ccagcagcca ggccggggcc
     3901 tctgccagag tgatttcccg gcaggcatgt tcggtgtacc catcaagttt gccctggtac
     3961 agcgccagcg tttcctggta cagctctggt gtcagggttc cctgtggaac ggcttcgcgg
     4021 gtaatgacca ggttgatgcc actgttatct gtggacacca ggacctgcat tgactgatcc
     4081 tgccattgcg atggcagctg aatatgacct tcattaaaag tgaactgcat aaaatactcc
     4141 ctgataatct gaatgaatca gttgatgaaa acagaaccgc ccacgatggt gatgccatcc
     4201 ttcgacagcc tgattaccgt cgattccccg acctgtagct gaatgccatc cggcgtcagc
     4261 gcaattttgc tttcctccac ggccagcata atttgcttaa ttgccgtata agcctgtata
     4321 ccgtcgcctt cgccaccatt gtttacagcg tcaacatgca ctgaaccggc aatcgtgcct
     4381 ctgtcctgaa aaacatcctc agataaagac ccttttagta cgcctgtgat tttgtggcct
     4441 ttttccaccg taagaaggcg gttaccttcc tggatcatga ccgtctggtc attttttacg
     4501 gtttcgcggt gatcgtgttt tacggtggtg gtacggtcgt tgagtacctc ggtatccatg
     4561 ttcttctggg cgtggatgta gacctgttct ttccctgtcg catcgtcaaa cttcagctcg
     4621 ttaaacccgc tgcccatata tgttttggag cggatggtca tctgcgtctt cgttcccggc
     4681 aggctgcccg gcgagcggtt atcttcgtgg taggtgcgcc ccataatgat cggctggtcc
     4741 ggatcgccgt tgaggaagtc cacaatcacc tcctgaccca cgcgcggtat cgccaggttg
     4801 ccaaatcccg gccccgccca cgcctgcgcc acccgtatcc agcacgaact gtcctgattg
     4861 gccgggttat agcggtccca gttgaatttc acccgcaccc ggccatgctc gtcgcagaag
     4921 atttcctctc ccgcaggccc cgtcacgacg gcgctctgcg gaccgtccac tgacggcttt
     4981 ggcttcggct gaggtcgcca cgtccggtct gccggtatta ccgcaaagtg gttattcagc
     5041 gtggtacctg accccctgcg tcccggcacc gcctgcggct gttcgccgtg cagttcgctc
     5101 gccaccacct gccattcccg gttcaggttc gcctgcggat gctccgtcag cacaattcgc
     5161 cgtccaggcc atatctccgg cgagcggctc attccccgcg cggtttcggc gttgtttcgc
     5221 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cgctcttgaa acgcccgggc
     5281 cagtcataca cttcatactg tgtgcgctgg taatcctgat gctggccttc ctggtcaaaa
     5341 cgtccggccc agccggggcg tttaaaggtg tagtctttgg tcaccacaga ggaaggacgg
     5401 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
     5461 gggttccagg ggatttcaaa ggattccggc agatagcgga ccgtgtcgca cagcaccaga
     5521 ctctggtcgg tacttttctg cgcgtgctcc tcatagaaga agatgccttc ctccgctgct
     5581 atccggcaga gaaagtcgta atcggtttca ccgtactgga cacaaaactc acgggaagga
     5641 tgcggctcac tgaacagcgg gctccactcc gtgacgtcat tttcctgcag tatggtgccg
     5701 aggatgcttc tgatgtcctc gttctggaat atacggaagt tctgacgcag tcctgcccgc
     5761 cacagcggcg ggcgcacctt catgctgtac agcatctggt ttttgtcgtt ctccccgagt
     5821 tcaaaccagg tcaccacgcc tttcacccgg cgctgtacgt catcgccctg ccataccgtc
     5881 aggtaggcca ttttgtccag cacctgcgca aattcaaggg agagaaactg ctgactgacc
     5941 agagaaaggt caagggaaaa aagtgaagag agtgactggg tgagatgaaa ggagaccacc
     6001 gcaaaagcgt ccggtggcag gccgtccact tccagtgtga aacgtaatcc ggttgacata
     6061 gcaacctccg tgaaaatgaa taataatccg catatattgc atgtacaaca tgtcgggtaa
     6121 tgagataagc gggccggctt caggcgttac cggggaatat cagaaaagtg tcatggttat
     6181 gtgatgattt tgtccgggat tttacttcag agaaacgggg ggctgttgac tcaacaaaat
     6241 aatcacaaac tcaactgacg ggttcacgat gttattaagg acaggctcgt atattacatc
     6301 gagtaacaaa aaaacacctg atatgcaaag tcaaaccaat atttgtaatg tctttttatt
     6361 cacaataata aataactcaa taaacattat tgaaatgaat gatggtttgt ataaggaata
     6421 tacgctaccg ccccaccctg ataacatgat ctgcacattg ctatcttaac gtcgttagac
     6481 gctggcttaa tagtgaatat tttaaattag gaatttatca agttcacttg ataaatagac
     6541 cgcgtgaata atatagagat taatgatgcc tgactgcgct ttatattata gccgattctt
     6601 ttatagtgat gaaaatttag taatagcatt gtgctctaca attggtagaa acacgaattt
     6661 aattaaattg catatctaaa tatctcgtaa atatccgaaa atgaataacc ttcgatttca
     6721 gacgaccaat aaggtcttcc ctgttttatt gataaataat aacagaaggc gatacatcac
     6781 tctttgagtt tgaactaact aattaacctt tctccggtag tttgatcata aaccttgtcc
     6841 cccgagcatc tgacgttact gcaaccgtgc ctttatgcgc gacaacaatc gatttcacta
     6901 tcgccaggcc aataccgcta ccttcgcctt ttcgctgacg ggaggggtca acgcgataga
     6961 aacggtcaaa caatcgcggt aagtgctcgg gcgcaatggg cgtaccgggg ttttcgacgg
     7021 taacttgcac ctggtgatcg accgtttggc agcgcacaac aattgcctct ccgggtggcg
     7081 tataacgcag ggcgttcgaa agcaggttgc ttaacgcccg acgcagcatc agcggatcgc
     7141 ccgcaacctg acactcgtca ccaacaaacc gcaactccac gccgcgatct tccgctaatg
     7201 cctcgaaaaa atcgaacact ttgccgactt catccgccag gttgagcatt ttcttttcgg
     7261 ggattaactg gttgttatcg gcctgggcga gaaacagcat atcgctgacc attttcgcca
     7321 ttcgcgtcag ctcttcgaga ttagagtaga gcacatcttc cagctccttc tggctgcgtg
     7381 actggctgag ggcgatttcc gtttgcgtta tgagattcgt aattggtgta cgaatttcgt
     7441 gggcgatgtc cgctgagaaa ttggactggc gggtaaagac atcctcaata cgctcgatca
     7501 tatggttgaa cgacagtacc agctgttcca gctcaatggg cacggtctgc gggtcaaggc
     7561 gaacgtcgag atctttcgag gtaatgttct ggatttgacg gctgacgctg cggatcggcg
     7621 cgtgaccttt atgtaccgcc aacagcacga taaagacgat caggatgctg atgatcgatg
     7681 cggtcataat aagtttattc atcaaatcat ttatgtaatg aagatgaaaa tcgatcgaaa
     7741 gcgcaatgta gagcgtataa atcggtttgc cgtccaccaa cgggccaacc ggcaagctaa
     7801 tcatccgcca gttgctgtgt tccatatgcc cgtgaccgtg gcccggcatc atcatcgtcg
     7861 ggccggaaag gagatacacc tcaccgcccc gcgtgtcttt atcgggtatg gcgtcacgcg
     7921 taaactcgcg gatatccggc gcaccggggg agtgatacac cgttttaccg tggctatctg
     7981 ccagggaaat caacacattg gaataaccac tgacgatatc ttccagcgtc attaagcgtt
     8041 gggcttgcgt ttcgtcaggg tgattaagta cccgttcaag agtggcgcta atctctttta
     8101 aatcattaat atcctgctcg gcaaaatgca cttttactga gtggatcatg atccatgcaa
     8161 aggcgaaaaa cgccgcgatg gtggccaggc taataaaaaa ggtcaggcgg gttgccagcg
     8221 aaaacgggcg ctgaaacggc ttactgacca tccggcacct caagcatgta acccacgccg
     8281 cgcacggttt gaatcagctt cggctcaaag tcgttgtcga ttttgccacg cagccgcttc
     8341 accgccacat caatggcgtt agtgtcgctg tcgaaattca tgtcccacac ctgtgaggca
     8401 ataagtgagc ggggcagtac ttcgccctga tggcgaagga aaaactccag cagagtaaat
     8461 tctttactgg tcagagtgat gcgcgtgcca ctgcgggtga ctttgcggct gacgagatcg
     8521 accatcagat cggcaacctg aaactgactt tcgataatca ccgccgcccc gcgccgcagt
     8581 agagtgcgca ctcgcgccag cagttcagca aaagcgaacg gcttcaccag atagtcatcc
     8641 gcccccaact ccagcccctt gacgcgatgt tcaatggtgc caagcgcggt aagcaacaga
     8701 atcggcatcc ccttattggc ggaacgcaac atgcgcacga tatcccagcc gttcacgtcc
     8761 ggcagcataa tatcgaggat tatcagatca taatcaccgg tcatcgccag atggtagcca
     8821 ttcagcccgt tgtcggccaa atcgaccaca aaaccggctt cggttaaccc tttggtcaag
     8881 tattctccgg ttttcttttc atcttcgaca atcaacagtt tcatatttcc tccgcatgtt
     8941 gcccgggcaa ttctagagta gcgggatcag atggcaatcg cttattggca aaatgacaat
     9001 tttgtcattt tcctgtcacc ggaaaatcag agcctggcga gtaaagttgg cggcataaaa
     9061 tcaccagaaa ttatgagcct atgtctcctt gtaaacttct gccattttgt gtggcccttg
     9121 cgctaaccgg ttgttcactg gcaccggatt atcagcgccc ggcaatgccc gtgccgcagc
     9181 agttctcact caaccagaac ggcctggtta acgcagcaga taactaccag aacgcgggct
     9241 ggcgcacctt ttttgttgat aatcaggtga agacgctgat tagcgaggcg ctggtgaata
     9301 accgggattt gcgcatggcg acgctgaaag tgcaggaagc gcgggcgcaa tatcgtctga
     9361 ccgatgccga ccgctaccca cagctcaatg gcgagggcag cggcagctgg agcaacaatc
     9421 ttaaaggcga ttcagccacg acgcgggagt tctcgactgg ccttaacgcc agctttgatc
     9481 tcgatttttt cgggcgctta aagaacatga gcgaagccga gcgccaaaat tatttagcca
     9541 ctgaggaagc tcagcgcgcg gtgcatattt tgctggtttc taatgtcgcg caaagctatt
     9601 tcaatcagca gctggcgtat gcgcaattgc aaatagccga agaaacgctg cgtaattatc
     9661 agcagtcata tgcgtttgtc gaaaaacaac tgttgacagg tagcagtaat gttctggcgc
     9721 tggaacaggc tcgcggggtg attgaaagta cccgcagcga catcgctaaa cgtcaggggg
     9781 aactggcgca ggcgaataat gcattgcaac tgttattggg aagctacggc aagctgccgc
     9841 aagcgcagac agtaaacagc gacagcctgc aaagcgttaa attaccggcg ggcttgtcgt
     9901 cgcaaatctt attgcagcgc ccggatatta tggaagctga acatgcgtta atggcggcta
     9961 atgccaatat tggtgctgca cgcgcggcat ttttcccgtc tatcagcctg accagcggaa
    10021 tatcaaccgc cagcagcgat ctgtcgtcat tatttaacgc cagcagcggg atgtggaatt
    10081 ttatacccaa aattgagatc cccattttta atgccggacg taaccaggcc aatctggata
    10141 tcgccgaaat tcgccagcag cagtcggtgg tgaattatga acagaaaatc cagaacgcct
    10201 ttaaagaagt ggcagatgcg ctggcattac gtcaaagcct gaacgatcaa attagcgccc
    10261 agcagcgtta tctggcgtcg ctgcaaatta ctttgcaacg ggcgcgggca ttatatcagc
    10321 acggcgcggt aagttatctg gaagtgctgg atgctgagcg ttctttattt gcaacccgac
    10381 aaactttact tgacctgaat tatgcccgtc aggttaatga aatttcttta tataccgcct
    10441 taggcggcgg ttggcagcaa taatttttaa ctccaggaga gaataaatga aaaaagcact
    10501 gcaagtcgca atgtttagtc tgtttaccgt tattggcttt aatgcccagg ctaacgaaca
    10561 tcatcatgaa accatgagcg aggcacaacc acaggtcatt agcgccaccg gtgtggtaaa
    10621 aaccattgat ctggaaagca aaaaaatcat catccaccac gatccaatta ccgcggtgaa
    10681 ctggccggag atgaccatgc gctttaccat caccccgcag acgaaaatga gtgaaattaa
    10741 aaccggcgac aaagtggcgt ttaattttgt ccagcagggc aacctttctt tattacagga
    10801 tattaaagtc agccagtaac ccaggtttaa tgagatgaaa aaaatcgcgc ttattatcgg
    10861 cagcatgatc gcgggcggta ttatttctgc ggcaggtttt acctggtttg caaaggcgga
    10921 accgcccgca gaaaaaacgt cgaccgcaga acgtaaaatc ttattctggt acgacccgat
    10981 gtatcccaat acgcggttcg ataaaccagg taaatcgccg tttatggata tggatctggt
    11041 gccgaaatat gccgatgaag agagttctgc gtctggtgta cgcattgacc cgacccagac
    11101 gcagaatctg ggggtgaaaa cggcgaccgt aacgcgcggg ccgctgactt ttgcccagag
    11161 tttcccggcg aatgtcagtt acaacgagta tcagtatgcc attgtgcagg ctcgcgctgc
    11221 cgggtttatc gataaggtgt atccgcttac cgtgggcgat aaagtacaaa agggcacacc
    11281 gcttctcgac ctgaccattc ccgactgggt ggaagcgcag agtgagtatt tactgctgcg
    11341 cgaaaccggc ggtacggcga cccagactga aggcattctt gagcgactgc gactggcggg
    11401 aatgccggag gcggatattc gccgcctgat tgccacgcaa aaaatccaga ctcgctttac
    11461 gctcaaagca cccattgatg gcgtgatcac cgcgtttgat ctgcgcgcgg gaatgaatat
    11521 cgccaaagat aacgtggtcg cgaaaattca gggtatggac ccggtgtgga tcactgccgc
    11581 gatcccggag tccatcgcat ggctggtgaa agatgcctcg cagtttacgc tcaccgttcc
    11641 ggcgcgacct gataaaacac tcaccattcg caaatggacc ctgctacccg gcgtggatgc
    11701 cgcgacccgc acgctgcagc tgcgtctgga agtcgacaac gccgacgagt cgctaaaacc
    11761 gggcatgaac gcctggttgc aactcaatac cgccagcgaa ccgatgctgc tcattccgtc
    11821 acaggcgctg attgataccg gcagtgaaca gcgggtgatt accgttgatg ccgacggacg
    11881 ctttgtaccg aaacgcgttg ccgttttcca ggcatcgcaa ggcgtcaccg cgttacgctc
    11941 tggtctggcg gaaggtgaaa aggtggtttc cagcggcctg ttcctgattg attccgaagc
    12001 caatatttct ggcgcactgg agcggatgcg ctctgaaagt gctacccatg cgcattgagg
    12061 gaataaccaa tgattgaatg gattattcgt cgctcggtgg cgaaccgttt tctggtgctg
    12121 atgggcgcgt tgtttctgag tatctggggc acctggacca tcattaacac gccagtggat
    12181 gcactgccgg atctctctga tgtgcaggtg attattaaaa ccagctatcc cggtcaggca
    12241 ccgcaaatcg ttgaaaatca ggtgacttat ccgctaacca ccaccatgtt gtcggtgcct
    12301 ggcgcgaaga ctgtgcgcgg tttctcgcag tttggcgact cttatgtgta tgtcattttc
    12361 gaagatggca ccgatccgta ctgggcgcgc tcgcgggtgc tggagtacct caaccaggta
    12421 cagggtaagc tgcctgcggg agtcagcgcc gagctggggc cagatgccac gggtgttggc
    12481 tggatctatg aatatgcact ggtggatcgc agcggtaagc acgatcttgc cgatttacgc
    12541 tcattgcagg actggtttct caaatatgag ctgaaaacca ttcctgacgt tgcggaagtg
    12601 gcgtcggtag gcggtgtggt gaaagagtat caggtggtta tcgatcctca gcgcctggcg
    12661 caatatggca tcagtctcgc cgaagtaaaa agcgcgctgg atgcttcaaa ccaggaagcg
    12721 ggtggttcgt cgatcgaact ggcggaagcg gaatatatgg tgcgagccag cggctatctg
    12781 caaacgctcg acgactttaa tcacatcgtt ttaaaagcca gtgaaaatgg cgtgcccgtt
    12841 tatctgcgtg atgttgcgaa ggtccagatt ggcccggaga tgcgccgggg cattgccgaa
    12901 ctgaacggcg aaggcgaagt ggcgggcggg gtggtgatcc tgcgctccgg caaaaacgcc
    12961 cgagaagtaa tcgccgccgt gaaggacaaa ctggaaacgc tgaaaagtag tctgccggaa
    13021 ggcgtggaga tagttacaac atacgatcgc agccagctca ttgatcgcgc catcgacaac
    13081 ctcagcggca agttgctgga agagtttatt gtggtggcgg tagtctgtgc gctgtttctc
    13141 tggcatgtgc gctcggcgct ggtggcgatt atttcgttgc cgctggggtt gtgtattgct
    13201 tttattgtca tgcactttca gggactgaac gccaatatta tgtcgttggg tggcattgcg
    13261 attgccgtcg gggcgatggt cgatgccgct atcgtcatga tcgagaatgc gcataaacgg
    13321 ctggaagagt ggcagcacca gcatcctgac gccacgttgg ataataaaac gcgctggcag
    13381 gtgattaccg atgcatctgt tgaagtgggg ccggcgctgt ttatcagtct gctgattatc
    13441 acgctgtcgt ttatcccgat cttcaccctg gaagggcagg aagggcgtct gtttggcccg
    13501 ctggcgttca ccaaaaccta tgcgatggcg ggtgcggcgc tactggcgat cgtagtgatc
    13561 ccgatcctga tgggctactg gatccgtggc aaaattccgc cggaaagcag taacccgctc
    13621 aatcgctttt tgattcgtgt ttatcatccg ctgttgctga aagtactgca ctggccgaaa
    13681 accacgctgt tggtggcagc gctttcggtg ctgacggttc tctggccgct caataaagtt
    13741 ggcggggaat ttttaccgca gatcaatgaa ggcgacttgt tgtatatgcc atcgacgctg
    13801 ccggggattt ccgcagcgga ggcggcgagt atgctgcaaa aaaccgacaa gctgattatg
    13861 agcgtaccgg aagtggcgcg ggtatttggc caaaccggga aagcggaaac cgccaccgat
    13921 tctgctccgc tggagatggt agaaacgacc attcagctta agccgcagga tcagtggcgg
    13981 cctggcatga cgatggacaa aatcattgag gaactggata acaccgtgcg tttgccgggg
    14041 ctggcgaatc tgtgggtgcc gccaattcgt aaccgtatcg atatgctctc gactggcatt
    14101 aaaagtccca tcggtattaa agtttccggc accgtgctgg cggatatcga cgcgatggcg
    14161 gagcaaattg aagaagtggc gcgaacggtg ccaggcgtgg cttctgcgct tgctgagcgt
    14221 ctggaaggtg ggcgttatat caacgttgag attaaccgtg aaaaagccgc gcgttacggt
    14281 atgacggtgg cggatgtgca gttgtttgtg acttctgcgg tgggcggggc gatggttggc
    14341 gaaacggtgg aagggattgc tcgttatcca attaatctgc gttatccgca aagctggcgc
    14401 gatagcccgc aggcactgcg ccagttgccg atcctcacgc cgatgaagca gcaaatcacc
    14461 ctggcagacg tggctgacgt taaagtctct accggaccgt cgatgctgaa aaccgagaat
    14521 gcgcgcccga cgagctggat ttatatcgat gcccgcgatc gtgacatggt gtcggtggtt
    14581 cacgatctgc aaaaagcgat agctgaaaaa gtgcagttaa aaccgggcac cagcgtggca
    14641 ttctccgggc agttcgagct actggagcgc gccaaccata agctgaaact catggtgccg
    14701 atgacgctga tgatcatctt cgtgctgtta tatctggcgt tccgtcgggt gggcgaagcg
    14761 ttgctgatta tcagcagcgt accgtttgcg ctggtgggcg gcatctggtt gctgtggtgg
    14821 atgggctttc atctttccgt ggcgacgggc accggtttta ttgcccttgc cggggttgcc
    14881 gccgaatttg gcgtggtgat gctgatgtat ttacgtcacg ccatagaggc cgagccgtcg
    14941 ttgaataatc cgcaaacatt cagcgagcag aagctggatg aggcgttgta tcacggcgcg
    15001 gtcctgcgcg tgcgcccgaa agcgatgacg gtggcagtga ttatcgccgg tctgctgccg
    15061 attctgtggg gaacgggggc tggttcagag gtgatgagcc ggattgccgc accgatgatt
    15121 ggcggcatga tcaccgcacc tttgctgtcg ctgtttatta tcccggcggc gtataagctg
    15181 atgtggctgc accgacatcg ggtacggaaa taaaagcagg ataccccgtt taaccgtgtg
    15241 gattgtgtct tgcgacgatg ggcactaaat gttaaaaggt gcccctcaac aaaaaagaca
    15301 cacaggggaa aggcgtgaaa aacgcgtcaa ccgtatcgga agatactgcg tcgaatcaag
    15361 agccgacgct tcatcgcgga ttacataacc gtcatattca actgattgcg ctgggtggcg
    15421 caattggtac tggtctgttt cttggcattg gtccggcgat tcagatggcg ggtccggctg
    15481 tattgctggg ctacggcgtc gccgggatca tcgctttcct gattatgcgc cagcttggcg
    15541 aaatggtggt tgaggagccg gtatccggtt catttgccca ctttgcctat aaatactggg
    15601 gaccgtttgc gggcttcctc tctggctgga actactgggt aatgttcgtg ctggtgggaa
    15661 tggcagagct gaccgctgcg ggcatctata tgcagtactg gttcccggat gttccaacgt
    15721 ggatttgggc tgccgccttc tttattatca tcaacgccgt taacctggtg aacgtgcgct
    15781 tatatggcga aaccgaattc tggtttgcgc tgattaaagt gctggcgatc atcggtatga
    15841 tcggctttgg cctgtggctg ctgttttctg gtcacggcgg cgagaaagcc agtatcgata
    15901 acctctggcg ttacggtggt ttttttgcca ccggctggaa tgggctgatt ttgtcgctgg
    15961 cggtaattat gttctctttc ggcggtctgg agctgattgg gattactgcc gctgaagcgc
    16021 gcgatccgga aaaaagcatt ccgaaagcag tgaatcaggt ggtgtatcgc attctgctgt
    16081 tttatatcgg ttcactggtg gttttactgg cactctatcc gtgggtggaa gtgaaatcca
    16141 acagtagccc gtttgtgatg attttccata atctcgacag caacgtggta gcttctgcgc
    16201 tgaacttcgt cattctggtg gcatcgctgt cggtgtataa cagcggggtt tactctaaca
    16261 gccgcatgct gtttggcctt tctgtgcagg gtaatgcacc gaagtttttg actcgcgtca
    16321 gccgtcgcgg cgtgccgatt aactcgctga tgctttccgg agccatcact tcgctggtgg
    16381 tgctgattaa ctatctgctg ccgcaaaaag cgtttggtct gctgatggcg ctggtggtag
    16441 caacgctgct gttgaactgg attatgatct gcctggcgca tctgcgtttt cgcgcggcga
    16501 tgcgacgtca gggacgtgaa acacagttta aggcgctgct ctatccgttc ggcaactatc
    16561 tttgcatcgc cttcctcggc atgattttgc tgctgatgtg cacgatggat gatatgcgct
    16621 tgtcagcgat tctgctgcca gtgtggattg tattcctgtt tgtagcgttt aaaacgctgc
    16681 gtcggaaata aggcattcgc gccgtatccg acaaaatgat gtcaaccatc cgaaaccgct
    16741 ctcatccatt cgatgagagc ggttttttta attactgctt aaatgcaccc gccagagagc
    16801 gaatatcatt gccagttggc gactgatgaa gtcgcagacc aaactcttcg acaatcgcaa
    16861 atatgtggtc gaatatatca gcctgaatgc tttcatattc cagccacacc acggtgttgg
    16921 taaacgcata gatctcgagc ggtaaaccgt tatcgccagg agccagttgg cgtaccatta
    16981 aggtcatatc tttacgaatc cgcggatggt tacgtagata ttcgttcaga taggcacgaa
    17041 aggttccaat attggtcatt cggcgcaggt ttaataccga ctccgtagaa ccttgctggc
    17101 gattccactc attaatttcc tgatggcggc tggttaaata aggctttaac aaatgcgctt
    17161 tattcagacg ttgcatttcg tcttcatcaa gaaaacgaat actggtgaca tcaatactga
    17221 tactgcgctt aatacgtcgc ccgccagatg ctgacatccc gctccagttt ttaaaggagt
    17281 cagaaaccag agaccaggtg ggaatagtgg taatggtatt gtcccagtta cgtactttga
    17341 cggtggttaa cccaatatcg atcaccgcgc catccgcgcc gtatttcggc atctccagcc
    17401 agtcgcccag tttcagcata tcgttcgcgg aaagctgaat acctgccacc agaccaagaa
    17461 tcggatcttt aaataccaac atcagcacgg cagccattgc accaagaccg ctgatcagaa
    17521 tcgctggcga ctgaccaatc agcagcgaga tcatcaaaat gccgaccaga atcgcgccga
    17581 tcagtttaat cccctgaaat atccctttca gcggtaactg agatgccgcc gggaatttct
    17641 gcgccagatt caaaatcaca tccagcaacg agaagactga aagcagcgca tacatcatga
    17701 tccacaactg cgcgcaggta gtcagaatat ctgccgcttc ggtgcctttt tgcagccaga
    17761 ataccgcctg aatattgacg ataatcccct gcagggtaaa agctaaacgg tggaagagtt
    17821 tattctgggt aatgatttgc aaccaaagcc gtgaactggc gatggcgcgt ttttcgaagg
    17881 tccgcagtac cacccagtgc aaaaaaatat gcaccacgac ggcggtcaga aaaataatac
    17941 cgaaaatcat caccatcgag gtggtgtgat cgatctcaat acccgctaaa tcttcaacct
    18001 gggatattaa atcctgcata acgtctcctt tatacaacag cagcctatga tgacggctga
    18061 aacagggtta tgcaaatcag gagaatctga ggggaaatag ccgggcagat gcccggcaag
    18121 ggagaattac acttcggtta aggtaatgtt ttgcggcaga cgagatttcg gcagcgtagc
    18181 gttaaaatct tcaacgctgt gatgacccac cggaacaacc accagactgg tgtagccttt
    18241 ctcttccaga ccaaattctt catcgaggat ggcggcgtca aaaccttcga tgggcaccgc
    18301 gtccagaccc agcgccgcca cgccgagcag gaagttaccg acgttgagat aaacctgttt
    18361 tgccatccac tctgcatcat cgtgcagatc tttacggtgc atatcggcga agaacttgcg
    18421 acctttatcg ttcgcggctt tcgcttccgg cgtggcaaag cggccatcgg catcttcctg
    18481 gtcaacaacc agcttcagcc aggcatcgtc catcgcggtt tttgcacaga acaccacgac
    18541 gtgcgaggca tcaagcattt tacgctcgtt gaacacgtaa ttaccggcag cggatttggc
    18601 aacacgcgct ttaccttctt ccgtgctggc aacaataaaa tgccacggct gggagttggt
    18661 actggatggg ctgtattgca ggagagtttt gacctgctcg gcctgttccg gggttagttt
    18721 tttgctggca tcaaatgcct tagtggaatg acgctttaag gcgacagaaa tgatatccat
    18781 aaagactcca tgtgaaagta attttgcgtg ccagcagatt acaaggttca acgagaaatg
    18841 gtaagcgaga aaaatgcgct atagatttcc gctttagcct ggacgcactc ttttttgatc
    18901 gcgtttagcc agaccatcaa ccaccagatt ccacgaatcg ttgatcagat cgcgaagtaa
    18961 cgcttcggag atttcctcgc cgggatacac tgaaatccag tgctttttat tcatgtgata
    19021 ccctggcttg atgctcgggt atatttgctg atttaacagg gatttttgtg gatcggactt
    19081 cagattgata aaggggacgc cgcgcagctc cgacgacagc ataaaaatct tgccgccaat
    19141 tttaaaaaca tcgaactccg ggccaaaagg ccagcaaagc tcgacaaagg gtaactcaag
    19201 ggccaggcgt ttcgccgttt cgtgcagtga ttgcttatcc ataaacgttc ctttaggcga
    19261 aggagaataa gcaaagtatg ccgcgaagta cggcgataat cgacgtttaa tccgccagcg
    19321 agaaccagcg tcgccagata aagcgcagaa caaaatactc aatagcgccc agcactaaaa
    19381 accacagaca aaacaataaa gtgtaaagct ggctaagatc catcagatgg aacatggtca
    19441 ccagtttttg tgccagcgtc agccccagtg cgggggcggg cagcagcagg caagaaagga
    19501 aagccatcag caaaatgcct gctgcggtgg tcaaggtttc taaagggtgt ttcataacat
    19561 tgttaaatgt aagttaaaac accattgtca gggatattct tctgtaaggc aattcccggc
    19621 ttagtcacca gcccagatct cacaatgctt tttcaccagc ccaatcagcg agccgccatc
    19681 ggcgacgaaa tcgcgcatca gctgcgcttc attcagaccg ctgacgacct ggcggtgcag
    19741 tgcttctatt gcgctcgatg cgccaatttt atgtgcagaa ggggcgattt tttccagtaa
    19801 acgcaaggtg tcttccatta gtgaccgacg atcgccagta tgcggatcgg taatgacgcc
    19861 ttccagccca tagcgacagg cctggaaacg gttgaattta tacagcaggt aatctttctc
    19921 ctgatgtttg aacgggcgtt ctgtcagtaa ccagtgggca gtagcctgaa ttaatcccgc
    19981 catatttacc gcatggctaa gggttaacgg ggtatccatt acccgaacct ccaccgtgcc
    20041 aaaatgagga ctggggcgaa tatcccagtg cagatcttta atgctgtcga tcatcgtggt
    20101 gtaactcaag cagcgaaaca gggcttcaaa ttgttgccag ttactgaccc acggcatcgg
    20161 gccattatcg ggaaaggcgg aaaaaatatt cggtcgtgag gaggcaaaac gcgtatccgt
    20221 tccttgcata tatggcgacg cggcggaaag ggcgataaag tgcggcacaa accgtgacag
    20281 gccgtgcagc aaataaatgg catcatcgcc actggcgcag ccaacatgga catgctgacc
    20341 aaaaacggtc gcctgctgga tgagatagcc aaagttttcc agcgttcgtt gatagcgttc
    20401 gttatcgcat acctcctgac gctgccattt ctgaaacggg tgcgtgccac cgccgcaaat
    20461 ttccagatga tggtctgcgg ctgcctgcaa tacgactttc tgcatcgctg aaaattgccc
    20521 ggcagcctgg ttgatatcac ggcaaacatc cgtcgccagc tccagcatac tttcggtgat
    20581 atcgtgcttt acctctccgg ccgtgatctt atttttaacc gcgtcaatca gcattgaaga
    20641 gtcctggctt aagtcatagc ccggcggatt aaccacctgc atttccagtt caataccgag
    20701 ggtaaaaggt tcagagactt gaaaatcggg tattggcata ggtttctctt atgttggcgt
    20761 tttctattca gtatagaagt cggagcggct gggcgagatg cggaagttct ggaatgtttc
    20821 tttttttgat gatggtgact gaagcaaatt ggctacattt tgcaatatga caagttatgg
    20881 cagtgcggac tggtggcgaa gaattttgac gattgaggca tgcagaataa aacgggttca
    20941 gcttcagttg atcaaatgag gattgagaag tccgaaacag gacttactgt ataaataaac
    21001 agccattttg ctaaggaagg gtaagataac ggcgggtgcc tgaagctttc cggtttcagg
    21061 tttactctga ggtctggaaa gatgaagccc caggagatat ttctatcaat cctggggctg
    21121 ccactccaaa cccaaacaat ttggatggta gtcccttctt tgcatggagg caatataaac
    21181 atgctgacga aatatgccct tgtggcggtc atggtgctgt gtttaaaggt aatttttatc
    21241 cggtttattc ggagcaatag tgattgcaag cgtatcaaga aaattcttat agaactgagc
    21301 aatttgttgc cagaatgacg atacaacctg atccagaagt aatatagaac gcccgtcgca
    21361 aacttgggga gtatgcagaa catggaatgc ctgagaagga taaccactcc agtcaacggc
    21421 aatgacgcac aacgtaaggt ctcggggctg aaaccgggta atattatgaa aaatagccgg
    21481 gatatcgcgg tgtaaagcgt cattgcctgg caggcgatcg atgggcttaa tcttgtttta
    21541 acctgagcat tgcctggcat ataccgtcca atgctggtaa ggatgaggga tgcgtcccga
    21601 ctaagcgcag cagtggcgtc aaggatggca ttctggcggt actgatgaaa tggagtcaaa
    21661 tcatcacgga aaaacttttg gcatgatgag cgaacgggca tagaggtgat ctcctgaatt
    21721 gttagcacaa ccagtcgacc acaaaactct atgcccgtct tttttacccg cctattactg
    21781 aggatttctc cgtaccatta ccggcatttg ttatctttca ttgctacata cgtcatcccc
    21841 caacaaagca atgctttata atattatcgt aatgtcttat tttttattat tgcgatggct
    21901 ttctgaacat tttatagtgt taggtgtaaa
//
LOCUS       DAZKGF010000080        21811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZKGF010000080 DAZKGF010000000
VERSION     DAZKGF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.080"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(115..1398)
                     /gene="gltA"
                     /locus_tag="ACHOQV_002804"
     CDS             complement(115..1398)
                     /gene="gltA"
                     /locus_tag="ACHOQV_002804"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFP9631150.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1604..1711)
                     /locus_tag="ACHOQV_002805"
     CDS             complement(1604..1711)
                     /locus_tag="ACHOQV_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631151.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2092..2496
                     /gene="sdhC"
                     /locus_tag="ACHOQV_002806"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2092..2496
                     /gene="sdhC"
                     /locus_tag="ACHOQV_002806"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP9631152.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2490..2837
                     /gene="sdhD"
                     /locus_tag="ACHOQV_002807"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2490..2837
                     /gene="sdhD"
                     /locus_tag="ACHOQV_002807"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFP9631153.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2837..4603
                     /gene="sdhA"
                     /locus_tag="ACHOQV_002808"
     CDS             2837..4603
                     /gene="sdhA"
                     /locus_tag="ACHOQV_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFP9631154.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASASDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4619..5335
                     /gene="sdhB"
                     /locus_tag="ACHOQV_002809"
     CDS             4619..5335
                     /gene="sdhB"
                     /locus_tag="ACHOQV_002809"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFP9631155.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5636..8437
                     /gene="sucA"
                     /locus_tag="ACHOQV_002810"
     CDS             5636..8437
                     /gene="sucA"
                     /locus_tag="ACHOQV_002810"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFP9631156.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8452..9669
                     /gene="odhB"
                     /locus_tag="ACHOQV_002811"
     CDS             8452..9669
                     /gene="odhB"
                     /locus_tag="ACHOQV_002811"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFP9631157.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPVKESAP
                     AAPAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9763..10929
                     /gene="sucC"
                     /locus_tag="ACHOQV_002812"
     CDS             9763..10929
                     /gene="sucC"
                     /locus_tag="ACHOQV_002812"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFP9631158.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10929..11798
                     /gene="sucD"
                     /locus_tag="ACHOQV_002813"
     CDS             10929..11798
                     /gene="sucD"
                     /locus_tag="ACHOQV_002813"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFP9631159.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11902..12624)
                     /gene="mngR"
                     /locus_tag="ACHOQV_002814"
     CDS             complement(11902..12624)
                     /gene="mngR"
                     /locus_tag="ACHOQV_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFP9631160.1"
                     /translation="MGRKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLTDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12794..14710
                     /gene="mngA"
                     /locus_tag="ACHOQV_002815"
     CDS             12794..14710
                     /gene="mngA"
                     /locus_tag="ACHOQV_002815"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFP9631161.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALNLKRSDETRTVQQDT
                     QPVKSLKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14728..17361
                     /gene="mngB"
                     /locus_tag="ACHOQV_002816"
     CDS             14728..17361
                     /gene="mngB"
                     /locus_tag="ACHOQV_002816"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFP9631162.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLTFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKLWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEITHEAWQSRAVIR
                     YDMAVPRNLHERSARQSTGRVGVELAVTLSDNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGLAVF
                     SEGLREYEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGSFSC
                     RLSLFSYTGTPSTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVVLRLFNPAESTTCEATVAFSREVISCTQTMMDESISTAERD
                     NLKELGAFLPGQSRTFSYRLG"
     gene            complement(17506..18903)
                     /locus_tag="ACHOQV_002817"
     CDS             complement(17506..18903)
                     /locus_tag="ACHOQV_002817"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HFP9631163.1"
                     /translation="MHHLSRLDKAFLRKGIQLSRANNIVSLSRPSGASARVLLPDELP
                     LEEKAVTQLLDFAEVKLPDHPGYVRQTCATPDFHPGNGVPVGAVVASTPDLVIPAAIG
                     TDISCGMRLLTTGLTLEQAEAHKDVIAQRLTQVLLQNERDVPLSAQAFTALFDGGPAD
                     CVSTLPSEGLWIRTNIPRMVQEIDRCIGLNEHNSHSRYAPPALQATDRALLRDPCLGT
                     PGSGNHFIELQVVDSVVDRHVAWQQGLKTGDIVVMIHSGSRDVGFYVGRHWMDRARAE
                     WPTGHKHPQSGLYGLAGGLAEEYLQAIGMAARYAWLNRVTIAEMVRVCLANLFHQDNS
                     HLVVDVPHNIILREHEMNIHRKGATPARVGNLALIPGSMGDYSWLAVGCGNPEWLWSC
                     SHGAGRSQRRQAMRSRATEESTLPWQCVTLREERRFEEAPAAYKDIGPVIEAQQEAGL
                     IQPAVRFRPWLTFKG"
     gene            complement(19293..19778)
                     /locus_tag="ACHOQV_002818"
                     /pseudo
     CDS             complement(19293..19778)
                     /locus_tag="ACHOQV_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="methylaspartate mutase subunit E; catalyzes the
                     formation of threo-beta-methyl-L-aspartate from
                     L-glutamate; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
     gene            complement(19797..21185)
                     /gene="glmL"
                     /locus_tag="ACHOQV_002819"
     CDS             complement(19797..21185)
                     /gene="glmL"
                     /locus_tag="ACHOQV_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFP9631164.1"
                     /translation="MQIVSVDIGSTWTKAALFAREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLDQVLNVDDALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQSYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     VVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSASANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAMVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(21185..21634)
                     /gene="glmS"
                     /locus_tag="ACHOQV_002820"
     CDS             complement(21185..21634)
                     /gene="glmS"
                     /locus_tag="ACHOQV_002820"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFP9631165.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
BASE COUNT         5238 a         5448 c         5856 g         5269 t
ORIGIN      
        1 taatgggtat ttgcatctgg tttaattttt acaatttatt atgttcagcc atataaaaaa
       61 gaacccgctc tattaaaggc gggtccggaa agtaaacggc ttagcaatca accattaacg
      121 cttgatgtcg cttttaaagt cgcgtttttc atatcctgta tacagttgac gcggacgggc
      181 aatcttcata ccgtcactgt gcatttcgct ccagtgggcg atccagccaa cggtgcgtgc
      241 catagcgaag atcacggtga acatagaaga cggaataccc atcgctttca ggatgatacc
      301 agagtagaaa tcgacgttcg gatagagttt cttctcgata aagtacgggt cgttcagcgc
      361 gatgttttcc agctccatag ccacttccag caggtcatct ttggtaccca gctctttcag
      421 cacttcatgg caggtttcac gcattacggt ggcgcgcggg tcgtaatttt tgtacacgcg
      481 gtgaccgaag cccatcaggc ggaaagaatc atttttatct ttcgcacgac gaacaaattc
      541 cggaatgtgt ttaacggagc tgatttcttc cagcattttc agcgccgctt cgttagcacc
      601 accgtgcgca ggtccccaca gtgaagcaat acctgctgcg atacaggcaa acgggttcgc
      661 ccccgaagag ccagcggtac gcacggtgga ggtggaggcg ttctgttcat ggtcagcgtg
      721 caggatcaga atacggtcca tagcacgttc cagaatcgga ttaacttcat acggttcgca
      781 cggcgtagag aacatcatat tcaggaagtt accggcgtag gagagatcgt tgcgcgggta
      841 aacaaatggc tgaccaatgg aatacttgta acacatcgcg gccatggtcg gcattttcga
      901 cagcaggcgg aacgcggcaa tttcacggtg acgaggattg ttaacatcca gcgagtcgtg
      961 atagaacgcc gccagcgcgc cggtaatacc acacatgact gccattggat gcgagtcgcg
     1021 acggaaagca tggaacagac gggtaatctg ctcgtggatc atggtatgac gggtcaccgt
     1081 agttttaaat tcgtcatact gttcctgagt cggtttttca ccattcagca ggatgtaaca
     1141 aacttccagg tagttagaat cggtcgccag ctgatcgatc gggaaaccgc ggtgcagcaa
     1201 aataccttca tcaccatcaa taaaagtaat tttagattcg caggatgcgg ttgaagtgaa
     1261 gcctgggtca aaggtgaata caccttttga accgagagta cggatatcaa taacatcttg
     1321 acccagcgtg cctttcagca catccagttc aacagctgta tccccgttga gggtgagttt
     1381 tgcttttgta tcagccattt aaggtctcct tagcgcctta ttgcgtaaga ctgccggaac
     1441 ttaaatttgc cttcgcacat caacctggct ttacccgttt gttatttggc tcgccgctct
     1501 gtgaaagagg ggaaaacctg ggtacagagc tctgggcgct tgcaggtaaa ggatccattg
     1561 atgacgaata aatggcgaat caagtactta gcaatccgaa ttattaaact tgtctaccac
     1621 taataactgt cccgaatgaa ttggtcaata cttccacact gttacataag ttaatcttag
     1681 gtgaaatacc gacttcataa cttttacgca ttatatgctt ttcctggtaa tgtttgtaac
     1741 aactttgttg aatgattgtc aaattagatg attaaaaatt aaataaatgt tgttatcgtg
     1801 acctggatca ctgttcagga taaaacccga caaactatat gtaggttaat tgtaatgatt
     1861 ttgtgaacgg cctatactgc cgccaggtct ccggaacacc ctgcaatccc gagccaccca
     1921 gcgttgtaac gtgtcgtttt cgcatctgga agcagtgttt tgcatgacgc gcagttatag
     1981 aaaggacgct gtctgacccg caagcagacc ggaggaagga aatcccgacg tctccaggta
     2041 acagaaagtt aacctctgtg cccgtagtcc ccagggaata ataagaacag catgtgggcg
     2101 ttattcatga taagaaatgt gaaaaaacaa agacctgtta atctggacct acagaccatc
     2161 cggttcccca tcacggcgat agcgtccatt ctccatcgcg tttccggtgt gatcaccttt
     2221 gttgcagtgg gcatcctgct gtggcttctg ggtaccagcc tctcttcccc tgaaggtttc
     2281 gagcaagctt ccgcgattat gggcagcttc ttcgtcaaat ttatcatgtg gggcatcctt
     2341 accgctctgg cgtatcacgt cgtcgtaggt attcgccaca tgatgatgga ttttggctat
     2401 ctggaagaaa cattcgaagc gggtaaacgc tccgccaaaa tatcctttgt tattactgtc
     2461 gtgctttcac ttctcgcagg agtcctcgta tggtaagcaa cgcctccgca ttaggacgca
     2521 atggcgtaca tgatttcatc ctcgttcgcg ctaccgctat cgtcctgacg ctctacatca
     2581 tttatatggt cggttttttc gctaccagtg gcgagctgac atatgaagtc tggatcggtt
     2641 tcttcgcctc tgcgttcacc aaagtgttca ccctgctggc gctgttttct atcttgatcc
     2701 atgcctggat cggcatgtgg caggtgttga ccgactacgt taaaccgctg gccttgcgcc
     2761 tgatgctgca actggtgatt gtcgttgctc tggtggttta cgtgatttat ggattcgttg
     2821 tggtgtgggg tgtgtgatga aattgccagt cagagaattt gatgcagttg taattggtgc
     2881 cggtggcgca ggtatgcgcg cggcgctgca aatttcccag agcggtcaga cctgtgcgct
     2941 gctctctaaa gtcttcccga cccgttccca taccgtttct gcgcaaggcg gcattaccgt
     3001 tgcgctgggt aatacccatg aagataactg ggaatggcat atgtacgaca ccgtaaaagg
     3061 ttcggactat atcggtgacc aggacgcgat tgaatatatg tgtaaaaccg ggccggaagc
     3121 gattctggaa ctggaacata tgggcctgcc gttctcgcgt cttgatgatg gtcgtatcta
     3181 tcaacgtccg tttggcggtc agtcgaaaaa cttcggcggc gagcaggcgg cacgtactgc
     3241 ggcggcagct gaccgtaccg gtcacgcact gttgcacacc ctttatcaac agaacctgaa
     3301 aaaccacacc accattttct ccgagtggta tgcgctggat ctggtgaaaa accaggatgg
     3361 cgcggtggtc ggttgtaccg cactgtgcat cgaaactggt gaagtggttt acttcaaagc
     3421 tcgcgctacc gtgctggcga ctggcggggc agggcgtatt tatcagtcca ctaccaacgc
     3481 ccacattaac actggcgacg gcgtcggcat ggctatccgt gcaggcgtac cagtgcagga
     3541 tatggaaatg tggcagttcc acccgactgg tattgccggt gcgggcgtac tggtcaccga
     3601 agggtgccgt ggtgaaggcg gttatctgct gaacaaacat ggcgaacgct ttatggagcg
     3661 ttatgcaccg aacgccaaag acctggcggg ccgtgacgtg gtggcgcgtt ccatcatgat
     3721 tgaaatccgt gaaggtcgcg gctgtgatgg tccgtggggg ccacacgcga aactgaaact
     3781 tgaccatctg ggtaaagaag ttcttgaatc ccgtctgccg ggtatccttg aactctcccg
     3841 caccttcgct cacgttgatc cggtgaaaga gccgattccg gttatcccaa cctgtcacta
     3901 catgatgggc ggtattccga ccaaagtgac cggtcaggcg ctgacggtga acgagaaagg
     3961 cgaagatgtg gttgttccgg ggctatttgc cgttggtgaa atcgcttgtg tatcggtaca
     4021 tggcgctaac cgtctgggcg gcaactcgct gctggacctg gtcgtatttg gtcgtgcggc
     4081 aggtctgcat ctgcaagagt ctatcgccga gcagggcgca ctgcgcgatg ccagcgcgtc
     4141 tgatgtagaa gcgtctctgg atcgcctgaa ccgctggaac aataaccgta acggtgaaga
     4201 tccggtggcg atccgtaaag cactgcaaga atgtatgcag cataacttct cggtcttccg
     4261 tgaaggtgat gcgatggcga aagggcttga gcagttgaaa gttatccgcg agcgtctgaa
     4321 aaatgcccgt ctggatgaca cttcaagtga gttcaatacc cagcgcgttg agtgcctgga
     4381 actggataac ctgatggaaa cggcgtatgc aacggctgtt tctgccaact tccgtaccga
     4441 aagccgtggc gcacatagcc gcttcgactt cccggatcgt gatgatgaaa actggctgtg
     4501 ccactccctg tatctgcctg agtcggaatc catgacgcgc cgaagcgtca acatggaacc
     4561 gaaactgcgc ccggcattcc cgccgaagat tcgtacttac taatgcggag acaggaaaat
     4621 gagactcgag ttttcaattt atcgctataa cccggatgtt gatgatgctc cgcgtatgca
     4681 ggattacacc ctggaagcgg aagaaggtcg tgacatgatg ctgctggatg cgcttatcca
     4741 gctgaaagag aaagatccca gcctgtcgtt ccgtcgctcc tgccgtgaag gtgtgtgtgg
     4801 ttccgacggt ctgaacatga acggcaagaa tggtctggcc tgtattaccc cgatttcggc
     4861 actcaaccag ccgggcaaga agattgtgat tcgcccgctg ccaggtttac cggtgatccg
     4921 cgatttggtg gtagacatgg gacaattcta tgcgcaatat gagaaaatta agccttacct
     4981 gttgaataat ggacaaaatc cgccagctcg cgagcattta cagatgccag agcagcgcga
     5041 aaaactcgac gggttgtatg aatgtattct ctgcgcatgt tgttcaacct cttgtccgtc
     5101 tttctggtgg aatcccgata agtttatcgg cccggcaggc ttgttagcgg catatcgttt
     5161 cctgatcgat agccgtgata ccgagactga cagccgcctc gacggtttga gcgatgcatt
     5221 cagtgtattc cgctgtcaca gcatcatgaa ctgcgtcagt gtatgtccga aggggctgaa
     5281 cccgacgcgc gccatcggcc atatcaagtc gatgttgttg caacgtaatg cgtaaaccgt
     5341 aggcctgaga agacgcgcaa gcgtcgcatc aggcaaccag tgccggatgc ggcgtgaacg
     5401 ccttatccgg cctacaagtc attacccgta ggcctgataa gcgcagcgca tcaggcgtaa
     5461 caaagaaatg caggaaatct ttaaaaactg cccctgacac taagacagtt tttaaaggtt
     5521 ccttcgcgag ccactacgta gacaagagct cgcaagtgaa ccccggcacg cacatcactg
     5581 tgcgtggtag tatccacggc gaagtaagca taaaaaagat gcttaaggga tcacgatgca
     5641 gaacagcgct ttgaaagcct ggttggactc ttcttacctc tctggcgcaa accagagctg
     5701 gatagaacag ctctatgaag acttcttaac cgatcctgac tcggttgacg ctaactggcg
     5761 ttcgacgttc cagcagttac ctggtacggg agtcaaaccg gatcaattcc actctcaaac
     5821 gcgtgaatat ttccgccgcc tggcgaaaga cgcttcacgt tactcttcaa cgatctccga
     5881 ccctgacacc aatgtgaagc aggttaaagt cctgcagctc attaacgcat accgcttccg
     5941 tggtcaccag catgcgaatc tcgatccgct gggactgtgg cagcaagata aagtggccga
     6001 tctggatccg tctttccacg atctgaccga agcagacttc caggagacct tcaacgtcgg
     6061 ttcatttgcc agcggcaaag aaaccatgaa actcggcgag ctgctggaag ccctcaagca
     6121 aacctactgc ggcccgattg gtgccgagta tatgcacatt accagcactg aagaaaaacg
     6181 ctggatccaa cagcgtatcg agtctggtcg cgcgactttc aatagcgaag agaaaaaacg
     6241 cttcttaagc gaactgaccg ccgctgaagg ccttgaacgt tacctcggcg caaaattccc
     6301 tggcgcaaaa cgcttctcgc tggaaggtgg tgacgcgtta atcccgatgc ttaaagagat
     6361 gatccgccac gctggcaaca gcggcacccg cgaagtggtt ctcgggatgg cgcaccgtgg
     6421 tcgtctgaac gtgctggtga acgtgctggg taaaaaaccg caagacttgt tcgacgagtt
     6481 tgccggtaaa cataaagaac acctcggcac gggcgacgtg aaataccaca tgggcttctc
     6541 gtctgacttc cagaccgatg gcggcctggt gcacctggcg ctggcgttta acccgtctca
     6601 ccttgagatt gtcagcccgg tcgttatcgg ttctgttcgt gcccgtctgg acagacttga
     6661 tgagccgagc agcaacaaag tgctgccaat caccattcat ggtgacgccg cagtgaccgg
     6721 gcagggcgtg gttcaggaaa ccctgaacat gtcgaaagcg cgtggttatg aagttggcgg
     6781 tacggtacgt atcgttatca acaaccaggt tggcttcacc acctctaacc cgctggatgc
     6841 ccgttcgacg ccgtactgta ctgatatcgg caagatggta caggcaccga ttttccacgt
     6901 taatgcggat gatccggaag ccgttgcctt tgtgacccgt ctggcgctcg atttccgtaa
     6961 cacctttaaa cgtgatgtct tcatcgatct ggtgtgctac cgccgtcacg gccacaacga
     7021 agccgacgag ccgagcgcaa cccagccgct gatgtatcag aaaatcaaaa aacatccgac
     7081 gccgcgcaaa atctatgctg acaagctgga gcaggaaaaa gtcgcgacgc tggaagatgc
     7141 caccgagatg gttaacctgt accgcgatgc gctggatgct ggcgattgcg ttgtagcaga
     7201 gtggcgtccg atgaacatgc actctttcac ctggtcgccg tacctcaacc acgaatggga
     7261 cgaagagtac ccgaacaaag ttgagatgaa gcgcctgcag gaactggcta aacgcatcag
     7321 cacggtgccg gaagcagttg aaatgcagtc tcgcgttgcc aagatttatg gcgatcgcca
     7381 ggcaatggca gccggtgaga aactgttcga ctggggcggt gcggaaaacc tcgcttacgc
     7441 cacgctggtt gatgaaggca ttccggttcg cctgtcgggt gaagactccg gtcgcggtac
     7501 cttcttccac cgccacgcgg tgatccacaa ccagtctaac ggttccactt acacgccgct
     7561 gcaacacatc cataacggtc agggcgcgtt ccgtgtctgg gactccgtac tgtcagaaga
     7621 agccgtgctg gcgtttgaat acggttatgc caccgcagaa ccacgcactc tgaccatctg
     7681 ggaagcgcag ttcggtgact tcgccaacgg tgcgcaggtg gttatcgacc agttcatctc
     7741 ctctggcgaa cagaaatggg gccggatgtg tggcctggtg atgttgctgc cgcacggtta
     7801 cgaagggcag gggccggagc actcctccgc gcgtctggaa cgttatctgc aactttgcgc
     7861 tgagcaaaac atgcaggttt gcgtaccgtc taccccggca caggtttacc acatgctgcg
     7921 tcgtcaggcg ttgcgcggga tgcgtcgtcc actggtcgtg atgtcgccga aatccctgct
     7981 gcgtcatccg ctggcggtat ccagcctcga agaactggcg aacggcacct tcctgccagc
     8041 catcggtgaa atcgacgagc ttgatccgaa gggcgtgaag cgcgtagtga tgtgttctgg
     8101 taaggtttat tacgacctgc tggaacaacg tcgtaagaac aatcaacacg atgtcgccat
     8161 tgtgcgtatc gagcaactct acccgttccc gcataaagcg atgcaggaag tgttgcagca
     8221 gtttgctcac gtcaaggatt ttgtctggtg ccaggaagag ccgctcaacc agggcgcatg
     8281 gtactgcagc cagcatcatt tccgtgaagt gattccgttt ggggcttctc tgcgttatgc
     8341 aggccgcccg gcctccgcct ctccggcggt agggtatatg tccgttcacc agaaacagca
     8401 acaagatctg gttaatgacg cgctgaacgt cgaataaata aaggatacac aatgagtagc
     8461 gtagatattc tggtccctga cctgcctgaa tccgtagccg atgccaccgt cgcaacctgg
     8521 cataaaaaac ccggcgacgc agtcgtacgt gatgaagtgc tggtagaaat cgaaactgac
     8581 aaagtggtac tggaagtacc ggcatcagca gacggcattc tggatgcggt tctggaagat
     8641 gaaggtacaa cggtaacgtc tcgtcagatc cttggtcgcc tgcgtgaagg caacagcgcg
     8701 ggtaaagaaa ccagcgccaa atctgaagag aaagcgtcca ctccggcgca acgccagcag
     8761 gcgtctctgg aagagcaaaa caacgatgcg ttaagcccgg cgatccgtcg cctgctggct
     8821 gaacataacc tcgacgccag cgccattaaa ggcaccggtg tgggtggtcg tctgacccgt
     8881 gaagatgtgg aaaaacatct ggcgaaagcc ccggtgaaag agtctgctcc ggcagcgcct
     8941 gctccggcgg cgcaaccggc cctggctgca cgtagcgaaa aacgtgtgcc gatgactcgc
     9001 ctgcgtaagc gtgtggcaga gcgtctgctg gaagcgaaaa actccaccgc catgctgacc
     9061 acgttcaacg aagtcaacat gaagccgatt atggatctgc gtaagcagta cggtgaagcg
     9121 tttgaaaaac gccacggcat ccgtctgggc tttatgtcct tctacgtgaa agcggtggtt
     9181 gaagccctga aacgttaccc ggaagtgaat gcgtctatcg acggtgatga tgtggtctac
     9241 cacaactatt tcgacgtcag tatggcggtt tctacgccgc gcggcctggt gacgccggtt
     9301 ctgcgtgatg tcgataccct cggcatggca gacatcgaga agaaaatcaa agagctggct
     9361 gttaaaggcc gtgacggcaa gctgacggtt gaagatctga ctggtggtaa cttcaccatc
     9421 accaacggtg gtgtgttcgg ttccctgatg tcaacgccta tcatcaaccc accgcagagc
     9481 gcaattctgg gtatgcacgc tatcaaagat cgtccgatgg cggtgaatgg tcaggttgag
     9541 atcctgccga tgatgtacct ggcgctgtcc tacgatcacc gtctgatcga tggtcgcgaa
     9601 tccgtgggct tcctggtaac gatcaaagag ttgctggaag atccgacgcg tctgctgctg
     9661 gacgtgtagt agtttaagct tcacctgcac tgtagaccga cgtggatagg gtgtcggtct
     9721 acggtttaaa agataacgat tactgaagga tggacagaac acatgaactt acatgaatat
     9781 caggcaaaac aactttttgc ccgctatggc ttaccagcac cggtgggtta tgcctgtact
     9841 actccgcgcg aagcagaaga agccgcttca aaaatcggtg ccggtccgtg ggtagtgaaa
     9901 tgtcaggttc acgctggtgg ccgcggtaaa gcgggcggtg tgaaagttgt aaacagcaaa
     9961 gaagacatcc gtgcttttgc agaaaactgg ctgggcaagc gtctggtaac gtatcaaaca
    10021 gatgccaatg gccaaccggt taaccagatt ctggttgaag cagcgaccga tatcgctaaa
    10081 gagctgtatc tcggtgccgt tgttgaccgt agttcccgtc gtgtggtctt tatggcctcc
    10141 accgaaggcg gcgtggaaat cgaaaaagtg gcggaagaaa ctccgcacct gatccacaaa
    10201 gttgcgcttg atccgctgac tggcccgatg ccgtatcagg gacgcgagct ggcgttcaaa
    10261 ctgggtctgg aaggtaaact ggttcagcag ttcaccaaaa tcttcatggg cctggcgacc
    10321 attttcctgg agcgcgacct ggcgctgatc gaaatcaacc cgctggtcat taccaaacag
    10381 ggcgatctga tttgcctcga cggcaaactg ggcgctgatg gcaacgcact gttccgccag
    10441 cctgatctgc gcgaaatgcg tgaccagtcg caggaagatc cgcgtgaagc acaggctgca
    10501 cagtgggaac tgaactacgt tgcgctggac ggtaacatcg gttgtatggt taacggcgca
    10561 ggcctggcga tgggcaccat ggacatcgtt aaactgcacg gcggcgaacc agctaacttc
    10621 ctggacgttg gcggcggcgc aaccaaagaa cgtgtaaccg aagcgttcaa aatcatcctc
    10681 tctgacgaca aagtgaaagc cgttctggtt aacatcttcg gcggtatcgt tcgttgcgac
    10741 ctgatcgctg acggtatcat cggcgcggta gcggaagtgg gtgttaacgt accggtcgtg
    10801 gtacgtctgg aaggtaacaa cgccgaactc ggcgcgaaga aactggctga cagcggcctg
    10861 aatattattg cagcaaaagg tctgacggat gcagctcagc aggttgttgc cgcagtggag
    10921 gggaaataat gtccatttta attgataaaa acaccaaggt tatctgccag ggctttaccg
    10981 gtagccaggg gactttccac tctgaacaag ccattgcata cggcactaaa atggttggcg
    11041 gcgtaacccc aggtaaaggc ggcaccaccc acctcggcct gccggtgttc aacaccgtgc
    11101 gtgaagctgt tgctgctact ggcgctaccg cttctgttat ctacgtacca gcaccgttct
    11161 gcaaagactc cattctggaa gctatcgatg caggcatcaa actgattatc accatcactg
    11221 aaggcatccc gacgctggat atgctgaccg tgaaagtgaa gctggatgaa gcaggcgttc
    11281 gtatgatcgg cccgaactgc ccaggcgtta tcactccggg tgaatgcaaa atcggtatcc
    11341 agcctggtca cattcacaaa ccgggtaaag tgggtatcgt ttcccgttcc ggtacgctga
    11401 cctatgaagc ggttaaacag accacggatt acggtttcgg tcagtcgacc tgtgttggta
    11461 tcggcggcga tccgatccct ggctctaact ttatcgacat cctcgaaatg ttcgaaaaag
    11521 atccgcagac cgaagcgatc gtgatgatcg gtgagatcgg cggtagcgct gaagaagaag
    11581 cagcggcgta catcaaagag cacgttacca agccagttgt aggttacatc gctggtgtga
    11641 ctgcgccgaa aggcaaacgt atgggccacg cgggtgcgat cattgccggt gggaaaggga
    11701 ctgcggatga gaaattcgct gctctggaag ccgcaggcgt gaaaaccgtt cgcagcctgg
    11761 cggatatcgg tgaagcactg aaaactgttc tgaaataaat atctgtaata agaaatagcc
    11821 ctcgccgctt ccttctacag gaatggcgaa gggctgtcgg tttcgacatg gttggccatc
    11881 gtatgatggc cttttttatg cttatcgcga tgattttcgc tgcgctatca gggtaaattt
    11941 atagtcatcg gtattaaaag cgttgcggct atattcaaac acccggccat caactaaata
    12001 tccacgcgat actttttcaa gaatcggctt agtctggctg atattaagca gacggctcat
    12061 ttcttcggtt ggcatcagag gaatgatttc ctgttcgcta cgatcgataa ccattttctt
    12121 cacttcttca ataaagtgat atttcgaatt ttccatgacc tgccaggtga gatccgggaa
    12181 caacgcaagc ggcatccagg tttcttccag cgccattggc ttttgcttgc gatagcgcac
    12241 gcgcttcaca tgccacacac gatcctgcgc ggtgatttgt agctgttgct gaagaaaatc
    12301 gtcagccgga atcacttcga atatcagaac ttcactgtgt gtatcgacgt gacggtccgt
    12361 cagtttttca tcaaaactgg ttaactgaaa aatatcgtaa ttgacccgct cttcttttac
    12421 gtaagtcccg ctgccctgaa tactttcgag gatctgctgt tcaactaact ggcgcaaagc
    12481 ctgacgcacc gtaacccggc tgacgccaaa ctctgtttgt agcgctgatt cagtgggtaa
    12541 cgcatcgcca ggtttaagct cgccacgcgc aatttgctca cgaatgcgat cagcaatctg
    12601 tcggtataag ggcttccgtc ccatgattta gcatctcatt aatacgaatt tagccattat
    12661 gcccgataaa ttcatcctgt aaataataca aatacaatac aaataatttc aatcaagtga
    12721 aattgatcac ataatggtat tgttttatcg ggcacactgg cgcgactata aaaacgatca
    12781 agtgaggatc atgatgaacc tgacgactct gacccaccgc gatgcgttgt gtctgaatgc
    12841 gcgctttacc agccgtgaag aggccatcca cgcgttgact caacgtcttg ctgctctggg
    12901 gaaaatttcc agtactgagc aatttctgaa agaagtgtat cgccgtgaaa gccttggccc
    12961 gaccgcctta ggtgaagggc tggctgtgcc gcatggcaaa actgctgcgg taaaagaagc
    13021 ggcgtttgcg gtcgccacac tcagcgagcc gcttcagtgg gaaggcgtgg atggcccgga
    13081 agcggttgat ttagtggtgt tgctggcgat tccccccaat gaagcgggta ctacgcacat
    13141 gcaactgctg acagcgctga ccacgcgcct ggcggatgat gagatacggg cgcgtataca
    13201 gtcggcgacg acgcctgatg aattgctctc ggcgctggat gacaagggag gcacgcaacc
    13261 ctcagcttct ttttccaacg cgccaactat cgtctgcgta acggcctgcc cggcgggtat
    13321 tgctcacacc tatatggctg cggagtatct ggaaaaagcc ggacgcaaac tcggcgtgaa
    13381 tgtctacgtt gaaaagcaag gtgctaacgg cattgaaggg cgtttaacgg cagagcaact
    13441 caatagtgca accgcctgta tttttgcggc tgaagtcgcc atcaaggaga gtgagcgttt
    13501 taacggcatt cccgcgcttt ctgtgcctgt tgccgagccg attcgccatg cagaagcgtt
    13561 gatccaacaa gcgcttaacc tcaagcgtag cgatgagacg cgtaccgtac agcaagatac
    13621 gcaaccggtg aaaagcctca aaacggagct aaaacaggca ctgttgagcg ggatctcttt
    13681 tgcagtgccg ttgattgtcg cggggggcac ggtgctggcg gttgcggtat tactgtcgca
    13741 aatcttcggg ctacaagatt tgtttaatga agaaaactcc tggctgtgga tgtaccgcaa
    13801 actgggcgga gggctgctcg ggattttgat ggtaccggtg ctggcagcct acaccgccta
    13861 ttcactggct gataaaccgg cgttagcgcc aggctttgcg gctggacttg ccgccaacat
    13921 gatcggttcc gggtttctcg gtgctgtcgt tggcggattg atagccggtt atctgatgcg
    13981 ctgggtgaaa aatcacttgc gtcttagcag taaattcaat ggattcctga ctttttatct
    14041 ctacccggtg ctcggtacgt tgggagcggg cagtctgatg ctgtttgtgg tgggggaacc
    14101 tgtcgcctgg atcaataact cgcttaccgc ctggctgaac ggtctgtcag gaagtaacgc
    14161 gctgttgctg ggtgccattc tcggttttat gtgttccttt gaccttggag ggccagtgaa
    14221 caaagccgct tatgcattct gcctgggcgc aatggcgaac ggcgtttacg gcccgtatgc
    14281 cattttcgcc tccgtcaaaa tggtttcggc atttaccgta accgcttcca cgatgctcgc
    14341 accgcgcctg tttaaagagt ttgaaattga gaccgggaaa tccacctggc tgttagggct
    14401 ggcaggtatt accgaagggg cgatcccgat ggcgattgaa gatccgctgc gggttattgg
    14461 ttcgtttgtg ctgggctcta tggtaacggg cgctatcgtc ggcgcgatga atatcggcct
    14521 ttcgacaccc ggtgccggca ttttctcgct ctttttactt catgataatg gcgcgggcgg
    14581 tgttatggcg gcaattggct ggtttggtgc ggcattggtg ggggctgcaa tctcgactgc
    14641 aattcttctg atctggcggc gtcacgcggt taagcatggc aactatctga ctgatggcgt
    14701 aatgccataa acaaaaagga aacgacgatg aaagcggtat ctcgcgttca catcaccccg
    14761 catatgcact gggatcggga gtggtatttc accaccgaag agtcacgcat tctgctggtc
    14821 aataatatgg aagagatcct gtgccgactg gaacaggaca acgaatacaa atattacgta
    14881 ctcgacgggc aaacggcgat cctcgaagat tatttcgcgg tgaaaccgga aaacaaagac
    14941 cgtgtgaaga aacaggtaga agcaggcaag ttgattatcg gcccctggta tacccagacc
    15001 gataccacga ttgtttctgc ggaatccatc gtccgtaatc tgatgtacgg aatgcgtgac
    15061 tgcctcacgt ttggcgagcc gatgaaaata ggttatttac cagattcctt tggcatgtcc
    15121 gggcaactgc cgcatattta caatggattt ggcattaccc gcaccatgtt ctggcgcgga
    15181 tgttctgagc gccacggtac tgataaaacc gagtttttgt ggcaaagcag tgacggtagc
    15241 gaagtgacgg cgcaggtgct gccgctgggc tacgccatcg gtaagtactt acctgccgac
    15301 gaagacggat tacgcaaacg cctcgacagt tattttgatg tgctggaaaa agcgtcagta
    15361 accaaagaga ttttgctgcc gaatggtcat gaccagatgc cattgcagca aaatatcttc
    15421 gaagtgatgg ataagctacg tgagatctac cctcaacgta agtttgtgat gagccgcttt
    15481 gaagaggtat ttgagaagat cgaagcgcag cgagataatc tggcaaccct gaaaggggaa
    15541 tttattgatg gcaaatatat gcgcgtgcat cgcaccatcg gttctacgcg tatggatatc
    15601 aaaattgccc acgcgcgtat tgaaaataag attgttaatc tgctggaacc gctggcaacg
    15661 ctggcctgga cgttgggttt tgaataccac cacggcttgc tggagaaatt gtggaaagaa
    15721 attttaaaaa accacgccca tgacagcatc ggctgctgct gtagtgacaa agttcatcgc
    15781 gagattgtcg cccgtttcga actggcagaa gacatggcgg ataatctgct gcgtttctat
    15841 atgcgtaaaa ttgccgataa catgccacag agtgatgccg acaaacttgt gctgtttaac
    15901 ctgatgccct ggccgcggga agaagtgatc aacaccactg tgcggctgcg cgccagccag
    15961 tttaatttgc gggacgatcg cggtcagcct gtaccgtatt ttattcgcca tgcccgtgag
    16021 atcgatcctg gcctaatcga tcggcaaatc gttcattacg gtaattatga tccctttatg
    16081 gagtttgata ttcagatcaa ccagattgtc ccttctatgg gctatcgcac gctttatatc
    16141 gaagcgaatc agcctggcaa cgtgattgcg gcaaaaagtg acgctgaagg gatactggaa
    16201 aatgctttct ggcaaattgc gctcaatgag gatggaacgc tacaactggt agataaagac
    16261 agcggtgtgc gctatgaccg ggtgttgcaa attgaagaaa gctctgatga tggtgatgaa
    16321 tatgactatt cacccgcaaa agaagagtgg gtaattactt cggcgaacgc gaaaccgcaa
    16381 tgcgagatta ctcatgaggc ctggcagagt agggctgtta tccgctatga catggcggtg
    16441 ccgcgtaatc tgcatgaacg cagcgcaaga caatccactg gcagagtagg ggtggagttg
    16501 gctgtcactc tgagtgataa cagcaggcgc attgatgtgg atatcaacct tgataaccag
    16561 gctgacgatc atcgccttcg cgtcctggtc cctacacctt ttaacaccga cagtgttctg
    16621 gccgataccc agtttggttc gctaacgcgc cccgtgaacg acagtgcaat gaacaactgg
    16681 cagcaagaag gctggaaaga agcgccggtt ccggtatgga atatgctcaa ctatgctgcc
    16741 ttacaggaag ggcgtaacgg tctggctgtc tttagcgaag ggctacgtga atatgaagtc
    16801 atcggtgaag agaagaaaac ctttgccatt acgttgctgc gtggcgtggg cttactgggg
    16861 aaagaagatc tgcttttaag gcctggacgt ccttcaggaa taaaaatgcc ggtgcccgat
    16921 tcacaattac gtggctcctt tagctgccgt ctgagcttat ttagttatac aggtacgcct
    16981 tcgaccgccg gtgtagccca gcaggcgcgc gcatggctga cgccagtaca gtgttacaac
    17041 aaaatcccgt gggatgcgat gaagttaaat aaagccggat tcaatgtgcc ggaaagctat
    17101 agcttgctga aaatgccccc tgtgggatgc ctgattagcg ctcttaaaaa agcggaagac
    17161 cggcaagaag tggtattgcg actgtttaat ccggcagaat cgacaacctg tgaggcaact
    17221 gtcgcgttca gtcgtgaggt gatttcatgt acgcaaacga tgatggatga aagcatcagc
    17281 acagcggaaa gagataattt aaaggaactg ggggcatttt taccggggca gtcacggaca
    17341 tttagctacc gccttggctg attattgcgc taaaagtgta tctgaggggg gcaataattt
    17401 caggccatct tcggatggcc tgagattgtt gataaacaag tttgttagct tacgcagata
    17461 ccagatttga cctgcgtgag aaccgcaggc catttatcat ttcagttacc ccttaaacgt
    17521 taaccacggt cgaaatctga ccgctggctg aattaatccg gcttcttgtt gggcttcgat
    17581 aaccggacca atatctttat aagccgctgg cgcttcttca aaacgtcgtt cttcgcgcaa
    17641 tgtgacgcat tgccacggca gcgttgactc ttcagtcgcg cgtgagcgca tggcctgccg
    17701 acgctggctg cgtcctgctc cgtgactgca tgaccacagc cactccgggt tgccgcaacc
    17761 taccgcaagc cacgaataat cgcccattga accggggatt aacgccagat tgcccactcg
    17821 tgccggtgtt gcgcctttgc gatgaatatt catttcatgc tcgcgaagga tgatgttatg
    17881 tggcacatcc accaccagat gcgaattgtc ctgatgaaag agattggcaa gacatacgcg
    17941 caccatttcg gcaattgtga cgcgatttaa ccatgcgtaa cgcgccgcca ttcctatcgc
    18001 ttgtagatat tcctccgcca gtccgcctgc cagaccatat aacccgcttt gtggatgttt
    18061 atgtccggtg ggccattcag cacgggcgcg atccatccag tgtcttccga cgtaaaagcc
    18121 aacatctcgc gatccactgt ggatcatcac cacaatatcg cctgttttca gcccttgttg
    18181 ccaggctaca tggcgatcaa ccacgctatc aacgacctga agttcaataa aatgattacc
    18241 tgaacccggc gtacccagac aaggatcgcg gagtaatgcg cggtccgttg cctgcagggc
    18301 gggtggggca taacgcgaat gactgttatg ctcgttaagc ccaatgcagc ggtcaatctc
    18361 ttgtaccatt cgggggatat tcgtgcgtat ccagagacct tctgatggca aggtcgacac
    18421 gcaatctgcc ggtccgccat caaaaagcgc ggtgaacgcc tgtgcggata acggaacatc
    18481 gcgctcattt tgcagtagca cttgcgttaa ccgctgagcg atgacgtctt tatgcgcttc
    18541 ggcttgctca agtgttaatc cggtcgtcag cagccgcatg ccacaagaaa tatcggtgcc
    18601 gatggcggcg ggaatgacta aatccggcgt tgaggccacc accgcgccaa cggggacgcc
    18661 attgccggga tgaaaatctg gcgtcgcgca cgtctggcgc acataacctg ggtggtctgg
    18721 tagcttcact tctgcaaaat caagcagttg tgttactgct ttttcttcca gcggcagttc
    18781 gtccggcagc agtacgcggg cactggcacc tgatggacga gataatgaaa caatattgtt
    18841 ggcgcgggaa agttggatcc ctttgcgcag aaaggcttta tcaagccttg aaagatgatg
    18901 cataacgctg tcctgaaata tagacgttaa acaaccccct gtatggcgag ggtaattttg
    18961 tctttccggt cagcagccag caaaagggaa agaaaacgcg cgtagtgtat gtggcgatag
    19021 caagtgttac aagtggtaaa tctggcttat tgttaaagag gaattatctt ttgtgaagtc
    19081 ttttaaggcc atgactcatt caatttaacc ggacatagat aatatataac atagggtatc
    19141 taaatttatc attgtatact tgtcataatt caagttgcat gtgctgcatc gatgttcgct
    19201 cacccaggtc acttacttat gtaagcttct ggaaattcat tatttgttcc cttcctgcaa
    19261 cttgacttat ttagagtata ttcatgtata cacatcgaga tttcagccag cagacgcgca
    19321 tctggcgtgc cgtggcggat ctgtagcggt ttttgcagag tttcggtgag gcgacggcaa
    19381 gctgcaacgc cgtggttaac gaccggtaaa ccgttcagtt tagaggttcc ggcttcgatg
    19441 gattttttaa tccctacagc cgcttcttcg taccgattca gacgggtata tgcatcgatg
    19501 gtgctcggca gcagatcgca ctcttcttgt agtgttttca gtagttcgat gtgttcgtcc
    19561 atcagcgcca cacctgcgcg cggttggcta agggttttac cttccttgtc tgctttcaac
    19621 agcgcaagag agaagcgttt atgctcagga atggtttgtt ggtatttaac gccatcttca
    19681 aagttttcaa catccttgcc ggtttcccag gttttcagta cctgttgccg ctcggtcatg
    19741 aattcgtcat gggttaattt cttatttcga agttccattg attgcagcct taattgttat
    19801 agggttaagc attgaatact ggtgcgcgcg gcggccagcg gatcgactct ggcgacgttt
    19861 gccagcagtg gtaatagccc ccgggaatcg cggtaatatt caaattcggt cggtaagaga
    19921 atgcgtttac cgtcgtcgtt taactcgcgg tatttcaacc agtgatgcat atcgaactga
    19981 ctggcacgcg agagccatcc gccggatccg acgactttac gcacggttgt caggtcacgt
    20041 cccatctgta aatcgacatt gccgacgcag gtgcagacct cttttttggt gcccgcatgt
    20101 ctttcggtgg cataacccac acacagtccg gccaggacgg tatcgaaata tttttcttct
    20161 gcattcgcgg gtaagtagtc gggatgagcg actagatgac ggaggtaatg ataaaaagcg
    20221 tccagctgtg cgggttgctg agcaaaattc acattgacta actctttcgc actttcacca
    20281 accaccattg ccgagacgcg cattcccaga tctccttcca ccgtccgttt tacgaagggt
    20341 tccggtacgc catgtaaaac agtgtcgggc gagagcgtgt tggcactggc ggaatagaca
    20401 tcggtggttg cgccacccat gtcgatcagc atgaactctt tccagctgtg atcgacatta
    20461 ctgattgcct taaccagttc aaagactgtc cacggtgtcg gcattggttc ttcgcctgtt
    20521 ttatcaacaa cgacatccag ccctttccct ttaacgatgc gtttaaggaa tatgtcgcag
    20581 atagcctggc gtgcagctaa agggttcgga tggtcaagat cgggcagaac gttgtcgacg
    20641 atggtcaggt ctttatgccc gaggatctcc tgcacctcgt cctgaatatc ccggtttccg
    20701 gcataaataa tggcgcagtc gagtttggat tctgccaaca cacgggcgtt attcagtccg
    20761 tagctttctt ctccgccatc ggtgccgccg gtgaacaaga gaatatcggg ctgcgtttct
    20821 tccagtgcct ggatatcacg gcggttcagc ttgtaggcgt aagattgcgc aattttagca
    20881 cccgcagagt gagcggttac tttcgcggtt tccagggtga tagaaggcac caatcccatt
    20941 gcggcaaccg ccagcccacc tttggcagag gaggaatact tcaatgcaac ctcaccactg
    21001 tttaacaggg ggagggcatc atctacgttt aacacctggt ccaggctgga gaaaaacccc
    21061 ttcgccagat gatgggtggt ggttggggtc agtacgtggt taaccaacgt taacgcgtcc
    21121 ccctcccggg cgaagagggc tgctttggtc cacgtcgatc caatatcgac agagacgatt
    21181 tgcatcagat tgcctcttca agaatacgag tatcaacgtc atgacgttgg ttgatatcgt
    21241 gggccatcag ctgacaaaca tcttctaagt catgacttgg ggcaaagacg cgatcaaatc
    21301 ccatctcttt gaatttggtt tccacgtcag cgaagtcatg ttttccgacc accaggttac
    21361 cgcccacata gagcaggatg tcccccagtc cacgctcgat gcagcgttcg cgcatgccca
    21421 gacagtcgat atcgccatgc ccatagatag aggagacgac aatcgcgtca gcaccagttt
    21481 caatggcagc atcaatatat tcgtcctggc tgaccattac gcccagatta ataactctga
    21541 agtcatggtt gctaaaaacg cggtccagaa ctttattgcc tactgcatgg cagtccgcgc
    21601 caataacgcc aatcacaagt gttgctttct tcatgggtaa tcctttgtaa gacagagagt
    21661 aaaataagtt acacacgtcg tatttatggc cgctcataac ggcacaggtt taatatcaca
    21721 ggagtgtctt tatctgaccg taatgttgca attgatctat tttcaaaatg tctttcggga
    21781 aatgattgtt taaagtgtgt tttcaaattc t
//
LOCUS       DAZKGF010000081        21773 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZKGF010000081 DAZKGF010000000
VERSION     DAZKGF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.081"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            42..1334
                     /locus_tag="ACHOQV_002821"
     CDS             42..1334
                     /locus_tag="ACHOQV_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9631166.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITVGQKAEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQTSQKKLDELVEQR"
     gene            1355..2236
                     /locus_tag="ACHOQV_002822"
     CDS             1355..2236
                     /locus_tag="ACHOQV_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9631167.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            2223..3065
                     /locus_tag="ACHOQV_002823"
     CDS             2223..3065
                     /locus_tag="ACHOQV_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9631168.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            3096..4148
                     /locus_tag="ACHOQV_002824"
     CDS             3096..4148
                     /locus_tag="ACHOQV_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP9631169.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCEIARRHGADFCLNPIGSDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            4167..4955
                     /locus_tag="ACHOQV_002825"
     CDS             4167..4955
                     /locus_tag="ACHOQV_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9631170.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVITACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEKVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALLEQLRADNFQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            4965..6020
                     /locus_tag="ACHOQV_002826"
     CDS             4965..6020
                     /locus_tag="ACHOQV_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9631171.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            6017..8284
                     /gene="ycjT"
                     /locus_tag="ACHOQV_002827"
     CDS             6017..8284
                     /gene="ycjT"
                     /locus_tag="ACHOQV_002827"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFP9631172.1"
                     /translation="MIRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELNFASGELR
                     RNVVWCTSNGAGYTITSRRFVSADQLPLIALEITITPLDADALVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKRVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQATGDESFIAHEGMALLLGTAK
                     FWISRAVQVNDRLEIHNVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARKFGCSDDA
                     FIHRAEIFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQYSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LAQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRNGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            8281..8940
                     /gene="pgmB"
                     /locus_tag="ACHOQV_002828"
     CDS             8281..8940
                     /gene="pgmB"
                     /locus_tag="ACHOQV_002828"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9631173.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFKAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     ADLRAQQIPIGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            8954..10036
                     /locus_tag="ACHOQV_002829"
     CDS             8954..10036
                     /locus_tag="ACHOQV_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9631174.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKERNIAMVFLNYALYPHMTVYDNMAFGLK
                     MQKIANEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTISEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            10081..10986
                     /gene="ompG"
                     /locus_tag="ACHOQV_002830"
     CDS             10081..10986
                     /gene="ompG"
                     /locus_tag="ACHOQV_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFP9631175.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(11037..12035)
                     /locus_tag="ACHOQV_002831"
     CDS             complement(11037..12035)
                     /locus_tag="ACHOQV_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9631176.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGNQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLIAGKPSPQNITVATHMSLK"
     gene            12190..13587
                     /locus_tag="ACHOQV_002832"
     CDS             12190..13587
                     /locus_tag="ACHOQV_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFP9631177.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13584..14645
                     /locus_tag="ACHOQV_002833"
     CDS             13584..14645
                     /locus_tag="ACHOQV_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFP9631178.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIEDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            14793..16334
                     /gene="tyrR"
                     /locus_tag="ACHOQV_002834"
     CDS             14793..16334
                     /gene="tyrR"
                     /locus_tag="ACHOQV_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFP9631179.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(16378..16884)
                     /gene="tpx"
                     /locus_tag="ACHOQV_002835"
     CDS             complement(16378..16884)
                     /gene="tpx"
                     /locus_tag="ACHOQV_002835"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFP9631180.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            17003..17968
                     /gene="ycjG"
                     /locus_tag="ACHOQV_002836"
     CDS             17003..17968
                     /gene="ycjG"
                     /locus_tag="ACHOQV_002836"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFP9631181.1"
                     /translation="MRTVKVFEEGWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQESLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(17943..18671)
                     /gene="mpaA"
                     /locus_tag="ACHOQV_002837"
     CDS             complement(17943..18671)
                     /gene="mpaA"
                     /locus_tag="ACHOQV_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFP9631182.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPL"
     gene            complement(18962..19600)
                     /locus_tag="ACHOQV_002838"
     CDS             complement(18962..19600)
                     /locus_tag="ACHOQV_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9631183.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKAGDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(19671..20591)
                     /locus_tag="ACHOQV_002839"
     CDS             complement(19671..20591)
                     /locus_tag="ACHOQV_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9631184.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNSRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSREYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            20729..21628
                     /gene="pgrR"
                     /locus_tag="ACHOQV_002840"
     CDS             20729..21628
                     /gene="pgrR"
                     /locus_tag="ACHOQV_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFP9631185.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT         5222 a         5272 c         5903 g         5376 t
ORIGIN      
        1 tccctggcag gttatgtgga ttaaataaaa aggaacattt catgattaaa tcaaaaatcg
       61 tgctgttatc agcactggtt tcctgcgccc tgatttcagg ctgtaaagaa gaaaataaaa
      121 ataatgtatc catcgaattt atgcattctt cggtggagca ggagcgccag gccgtcatca
      181 gcaaattgat tgcccgtttt gaaaaagaaa acccaggtat caccgtaaaa caagtgcccg
      241 tggaagaaga cgcctataac accaaagtca ttaccctttc acgtagcggt tcgctgccgg
      301 aagtgatcga aaccagccat gactacgcca aagtgatgga caaagagcag cttatcgatc
      361 gtaaagcggt tgccacggtc atcagcaacg tcggtgaagg cgcgttctac gatggcgtac
      421 tgcggattgt gcgcaccgaa gatggcagcg cctggaccgg tgttcctgtc agcgcatgga
      481 ttggcgggat ctggtatcgc aaagatgtgc tggcaaaagc tgggctggag gagccaaaaa
      541 actggcagca attgctggac gttgcacaga aactgaatga cccggcgaat aaaaaatacg
      601 gtattgcgct gcctacagca gaaagcgtgt tgacggaaca gtctttctcc cagtttgcgt
      661 tatccaatca ggctaacgtc tttaacgccg aaggcaaaat cacccttgat acaccagaga
      721 tgatgcaggc gctgacctat taccgcaacc ttgctgccaa caccatgcca ggttctaacg
      781 acatcatgga agtgaaagac gcttttatga acggcaccgc gccgatggcg atttactcca
      841 cctatatcct tccggctgtg attaaagaag gcgatccgaa aaacgttggt ttcgttgtac
      901 ccaccgagaa aaactctgcg gtctacggca tgttgacctc gctgaccatt accgtcgggc
      961 aaaaggccga agagacggaa gcggctgaaa aatttgtcac ctttatggag caggcagaca
     1021 acattgccga ctgggtgatg atgtcgccag gcgcggcgct gccggtgaac aaagcggttg
     1081 tcactaccgc cacctggaaa gacaacgacg ttattaaggc gctgggtgag ctgccaaatc
     1141 agttaatcag cgaactgcca aatattcagg tctttggcgc agtaggggat aaaaatttta
     1201 cccgcatggg cgatgtgacg ggttctggcg tggtgagctc catggtgcat aacgtcaccg
     1261 tgggtaaagc cgacctccct ggcacgctgc aaacgagcca gaaaaagctg gatgagttgg
     1321 ttgaacagcg ctaaacccag gacaggaatc cgcaatgaac aggctttttt caggtcgttc
     1381 cgatatgccc tttgcgctgt tgcttctcgc gcccagctta ttactgctgg gcggtctggt
     1441 ggcgtggccg atggtgtcga atatcgaaat cagtttttta cgtctgccgc ttaatcccaa
     1501 catcgagtca acgtttgttg gggtgagcaa ctacgtgcgt attctctccg atcccggctt
     1561 ctggcactcg ctgtggatga cggtctggta taccgcgctg gtggtggcgg gcagcaccgt
     1621 gctcgggctg gccgtggcga tgtttttcaa ccgcgagttc cgcctgcgca aaaccgcgcg
     1681 ttcgctggtg atcctctcct acgtaacgcc gtctatttcg ctggtgttcg cctggaaata
     1741 catgttcaac aacggttacg gcattgtgaa ctacctcggc gtcgatcttc tgcatctcta
     1801 tgagcaggca ccgctgtggt tcgataatcc gggcagtagc tttgtgctgg tggtgctgtt
     1861 cgccatctgg cgctacttcc cctatgcctt tatttcgttt ctggcgattt tgcagaccat
     1921 cgacaaatcg ctgtacgaag cggcagagat ggatggcgct aatgcctggc aacggtttcg
     1981 tatcgtcacg ctgcccgcga ttatgccggt cctggcgacg gtggtgaccc tgcgcaccat
     2041 ctggatgttc tacatgttcg cggatgttta tctgctgacg accaaggtcg atattctcgg
     2101 tgtctatctc tacaaaaccg cctttgcctt taatgactta gggaaagcgg cggcgatctc
     2161 ggtggtgctc ttcatcatta ttttcgctgt cattctgctg accagaaaac gggtgaacct
     2221 caatggcaac aaataaacgc acactcagtc gcatcggttt ttactgcggg ctggcgctgt
     2281 ttctcatcat cacgctgttt ccgttttttg tgatgctgat gacctcgttc aagagcgcaa
     2341 aagaggcgat ctcgctgcat cctacgctgc tgccgcaaca gtggacgctg gagcattatg
     2401 tcgacatttt taacccgatg attttcccgt ttgtcgacta cttccgtaac agcatggtgg
     2461 tgtcggtggt ttcatccgtg gtggcggtat ttctcggcat tctcggggct tacgcgcttt
     2521 cccgcctgcg ctttaaaggg cggatgacca tcaacgccag cttttacacg gtgtatatgt
     2581 tctccggcat tttgctggtg gtgccgctgt tcaaaatcat caccgcgctt ggcatatacg
     2641 acaccgagat ggcgctgatc attaccatgg taacgcaaac cctgcccacc gccgtgttta
     2701 tgctgaaaag ctacttcgac accatccctg atgagatcga agaagcggcg atgatggatg
     2761 gcctcaaccg tctgcaaatc atcttccgta tcaccgtgcc cctggcgatg tccgggctga
     2821 tatccgtatt cgtctactgc tttatggtgg catggaacga ctatctgttt gcgtcgattt
     2881 tcctctccag cgccagcaat ttcaccttac cagtcggcct gaacgcgctg ttcagtacgc
     2941 ctgattacat ctggggacgg atgatggcgg cctcgctggt gaccgcattg ccggtggtga
     3001 ttatgtatgc gctttcggag cgttttatta agagtggttt gaccgccggt ggcgtgaagg
     3061 gctaaagcgg ccagtttctt tacaaggagt tttacatgaa aaagttagta gccacagcac
     3121 cgcgcgtagc tgcgctggtt gagtatgaag atcgggcgat tttagccaat gaagtgaaga
     3181 tccgcgtgcg cttcggcgca ccgaagcacg ggacggaagt ggtcgatttc cgcgctgcca
     3241 gtccgtttat cgatgaagac tttaacggcg aatggcagat gttcaccccg cgtccggcag
     3301 atgcgccgcg cggcattgag tttggcaaat tccagcttgg caacatggtg gttggcgaca
     3361 ttatcgagtg cggcagcgac gttaccgact acgcggtggg cgacagcgta tgcggctacg
     3421 gcccgctctc cgagacggtc atcattaacg cagtgaataa ctacaagctg cgcaaaatgc
     3481 cgcaaggcag ttcgtggaaa aacgctgtct gctacgaccc ggcgcagttt gccatgagcg
     3541 gcgtgcgcga tgccaatgtg cgcgtgggcg attttgtggt ggtggtaggg cttggcgcga
     3601 tcggtcaaat tgccattcaa ctggctaaac gtgctggcgc gtcggtggtg attggcgtcg
     3661 atcctatcgc ccatcgctgt gagattgccc gtcgccatgg cgcggatttc tgccttaacc
     3721 ccattggcag tgacgtaggc aaagagatca aaacgctaac cggcaagcag ggtgccgatg
     3781 tgattatcga aaccagcggc tatgccgacg cgctgcaatc ggcgctacga ggtctggcat
     3841 atggcggcac catctcgtat gtcgcgtttg ccaaaccgtt tgccgaaggt tttaacctcg
     3901 gacgcgaagc ccatttcaat aacgccaaaa ttgttttctc ccgtgcgtgc agcgagccga
     3961 acccggatta tccgcgctgg agccgcaaac gtatcgaaga aacctgttgg gaactgctga
     4021 tgaacggtta tctcaattgt gaagatttaa tcgacccggt ggtgaccttt gccaatagcc
     4081 cggaaagtta catgcagtat gtcgaccagc atccggaaca gagcatcaaa atgggcgtta
     4141 cgttttaatt caaaggaatg aaaaaaatga aaatcggcac acagaatcag gcgttctttc
     4201 cggaaaacat tctggagaaa tttcgttata tcaaagagat gggtttcgat ggttttgaga
     4261 tcgacggcaa actgctggtc aacaatctcg aagaagtcaa agcagcaatc aaagaaaccg
     4321 gtttaccggt gatcaccgcc tgcggtggct atgacggttg gattggcgac tttatcgaag
     4381 agcgtcgact taatggctta aagcagatcg aacgcattct tgaagctctg gcagaagtgg
     4441 gcggtaaagg tatcgtcgtt ccggctgcat ggggcatgtt taccttccgc ttaccgccga
     4501 tgacctcgcc gcgtagcctg gacggcgacc gcaaaatggt gagtgattcc ctgcgcgtac
     4561 tggaaaaggt ggccgcacgt accggaaccg tggtgtacct cgaaccgtta aaccgttatc
     4621 aggatcacat gatcaacact ctcgccgatg cccgccgtta tatcgtcgaa aacgatctta
     4681 aacatgtaca gattatcggc gatttctatc acatgaatat cgaagaagat aacctggcgc
     4741 aggcgctgca tgacaaccgc gacctgctcg gtcatgtgca tattgccgat aaccatcgct
     4801 accagccggg cagcggcacc ctggatttcc acgcgctgtt agaacagctg cgcgcggata
     4861 actttcaggg ctatgtggtg tacgaagggc gcatccgggc ggaagatcca gcccaggcgt
     4921 accgtgattc tttggcctgg ttgcgtacct gctaagaggt ctttgtgaaa agtgcaatga
     4981 caagctctcc gctgcgggtc gcgataatag gcgcgggcca ggtggcggat aaagttcatg
     5041 cttcgtacta ctgcacccgc aacgatctgg aactggtggc tgtctgtgac agccgccttt
     5101 cccaggcgca ggcgctggca gaaaaatacg ggaatgcatc cgtgtgggac gatccgcagg
     5161 ccatgctgct ggcggtgaaa cctgatgtgg ttagcgtctg ctcacctaac cgttttcatt
     5221 acgaacatac cctgatggcg ctggaagcgg gctgccatgt gatgtgcgaa aaaccgcccg
     5281 ccatgacggc agaacaggcg cgggaaatgt gcgataccgc gcgcaaacag ggcaaggtgc
     5341 tggcctacga ctttcaccat cgttttgcac tcgatacgca acagctgcgt gaacaggtga
     5401 ccaacggcgt tttgggagag atttacgtta ccaccgcccg cgccctgcgt cgttgcggtg
     5461 ttcccggctg gggtgtcttt actaataaag aattgcaggg cggaggcccg ctgatcgaca
     5521 tcggcattca tatgctggat gcggcgatgt atgtgctggg ttttccagcg gtgaaaagcg
     5581 tgactgcgca tagctttcaa aagatcggca cgcaaaagag ctgcggtcaa tttggcgagt
     5641 gggacccggc aacctacagc gtcgaagatt cgctgtttgg caccattgaa tttcataacg
     5701 gcggcattct gtggcttgag acctcatttg cactcaacat ccgcgaacag tcgattatga
     5761 acgtcagctt ttgtggtgat aaagccgggg cgacgctgtt tccagcacat atctacaccg
     5821 ataacaacgg tgagttaatg acgctgatgc aacgggaaat ggcagacgac aaccgtcatc
     5881 tgcgcagtat ggaagccttt atcaaccacg tacaaggtaa gcccgtgatg atagccgacg
     5941 ccgagcaggg atacatcatc cagcaactgg tggcggcgct ttatcaatcc gcagaaacag
     6001 ggacgcgtgt ggaattatga tcaggccagt aacgttaacg gaaccccatt tcagccagca
     6061 taccctgaac aagtatgctt cgctgatggc gcaggggaac ggctatcttg ggcttcgcgc
     6121 cagccatgaa gaagattaca cgcgccagac gcgcggcatg tatctggcag ggctttatca
     6181 tcgggcgggt aaaggtgaaa tcaacgaact ggtgaacctg cctgatgtcg tggggatgga
     6241 gattgccata aatggtgaga ttttctcgtt atcccgcgaa gcatggcagc gtgagcttaa
     6301 ctttgccagt ggcgaattac gccgcaacgt tgtctggtgt accagcaacg gcgcaggtta
     6361 caccatcacc agccgtcgct ttgtttcggc agaccaactg ccgctcattg cgctggaaat
     6421 cactattacg ccactggacg ccgacgcgtt agtgctgatt tcaacaggca ttgacgccac
     6481 gcaaaccaac cacggtcgcc aacatctcga cgaaacccag gtgcgggtat ttggtcagca
     6541 tctgatgcag gggatctaca ccacccagga tgggcgcagt gatgtggcca tcagctgttg
     6601 ctgtaaggtg agcggtgatg tgcagcaatg ctataccgcc aaagagcgcc gtttactgca
     6661 acatacctgc gcgcagcttc acgcaggtga gacgctgacg ctgcaaaaac gggtgtggat
     6721 cgactggcgg gacgacagac aagccgcttt agacgaatgg ggcagtgcgt cgcttcgtca
     6781 gcttgaaatg tgcgcgcagc agagttacga ccaacttctt gcagcatcaa cagaaaactg
     6841 gcgtcaatgg tggcagaaac gtcgtatcac ggtaaacggc ggcgatgcgc acgatcagca
     6901 agcgttagat tatgcgcttt atcacctgcg catcatgacg cctgcccacg atgagcgcag
     6961 cagcattgcg gcgaaaggtt taaccggcga aggctacaaa ggccacgttt tctgggatac
     7021 agaagtattt ttgctaccgt tccatctgtt tagcgatccg acggttgccc gaagtttact
     7081 gcgttatcgc tggcacaact tgcctggcgc gcaggagaaa gcgcgacgca acggctggca
     7141 gggcgctctg tttccgtggg aaagcgcgcg cagcggcgaa gaagagacgc cggaatttgc
     7201 cgccattaac atccgcaccg gactgcgcca gaaagtggcc tcggcgcagg ctgaacacca
     7261 tttggtggct gatatcgcct gggcggttat tcaatactgg caggcaacgg gggatgaaag
     7321 tttcattgcc cacgaaggca tggcgttact tctgggaacc gcaaaattct ggattagccg
     7381 cgctgtacag gtaaacgatc gtctggaaat tcacaatgtt attgggccag acgaatatac
     7441 cgaacatgtc aataataacg ccttcaccag ctatatggcg tattacaacg tccagcaggc
     7501 gctgaatatc gctcgtaagt ttggttgtag cgacgacgcg tttattcatc gtgccgaaat
     7561 attcctcaaa gaactgcggc tgccagaaat tcagcctgac ggcgttttgc cgcaggatga
     7621 ttcgtttatg gcgaaaccag cgattaatct ggcgaaatac aaagcggcgg cggggaagca
     7681 aaccattctg ctggattatt cacgcgcaga agtgaacgag atgcagatcc tcaaacaagc
     7741 tgatgtggtg atgctcaatt acatgctgcc ggagcagtac tcagcggcat cgtgtcttgc
     7801 caatctgcaa ttttatgaac cgcgcaccat tcacgactct tcactgagta aagcgatcca
     7861 cggcattgtt gccgcacgct gtggcctgtt ggcgcaaagt tatcagttct ggcgcgaggg
     7921 gactgaaatc gatcttggtg ctgatccgca tagctgtgat gacggtatcc acgcagccgc
     7981 aactggcgct atttggctgg gggcgattca gggttttgcc ggggtgagcg tgcgtaacgg
     8041 tgaattgcat ctcaatccag cgttacctga gcagtggcaa cagttgtctt tccctctgtt
     8101 ctggcagggc tgcgaattac aggtcactct cgacgcgcag cgtattgcga ttcgaacttc
     8161 tgcgcccgtt tcactgcgtt tgaacgggca gcttatatct gtggctgaag aatctgtttt
     8221 ctgtttgggt gattttattt tgcccttcaa tgggaccgct accacgcatc aggaggatga
     8281 atgaaactgc aaggggtaat tttcgatctg gatggtgtaa tcaccgatac cgcgcatctg
     8341 catttcaagg cgtggcagca gattgccgct gaaattggca tcagcattga tgcgcagttt
     8401 aacgaatccc taaaagggat cagccgcgat gagtctctgc ggcgcattct gcaacacggg
     8461 ggcaaagagg gcgactttaa cccgcaggag agggcgcaac tggcgtatcg caaaaatctg
     8521 ctctacgtcc actcactgcg cgaactgacg gttaatgctg tgctgcccgg cattcgcaat
     8581 ttgctggcag atctccgtgc acagcagatc cctatagggc tggcttctgt ctccctgaat
     8641 gcgccgacga ttttagcagc gctggagctg cgcgagtttt tcaccttctg cgcggacgct
     8701 tcccaactta aaaactcgaa accggacccg gaaatctttc tcgccgcctg tgccggactg
     8761 ggcgtgccgc cgcaggcatg tatcggcatt gaagatgcgc aggcgggcat tgacgccatt
     8821 aacgccagcg gtatgcgctc ggtggggatc ggcgcgggct taaccggggc gcaattattg
     8881 ttgccctcaa cggactccct gacctggccg cggttatcgg ccttctggca aaacgtatag
     8941 caaaggaatc aacatggctc agctttcgtt acaacatatt caaaaaatct acgataacca
     9001 ggtgcatgtg gtgaaggact tcaacctgga aattgccgat aaagagttca ttgtctttgt
     9061 cggtccgtca ggctgcggta aatcgactac gctgcgcatg attgccgggc ttgaggagat
     9121 cagcggcggc gatctgttga tcgacggcaa acgaatgaat gacgttccgg ctaaagagcg
     9181 caatatcgcg atggtgttcc tgaactacgc gctgtatccg catatgacgg tctacgacaa
     9241 catggcgttt ggcctgaaga tgcaaaaaat cgccaacgag gtgattgatg agcgggtgaa
     9301 ctgggcggcg caaattctcg gcctgcgtga gtacctgaaa cgcaagccgg gggcgctttc
     9361 cggcgggcaa cgtcagcgag tggcgctcgg gcgggcgatt gtgcgcgaag cgggcgtgtt
     9421 tttaatggat gaaccgctct ctaacctgga tgccaagctg cgcgtgcaaa tgcgcgcaga
     9481 gatcagcaag ctgcatcaga aactgaacac caccatgatc tacgtgaccc acgatcagac
     9541 cgaagcgatg accatggcga cgcggattgt gattatgaag gatgggattg ttcagcaagt
     9601 aggtgcgccg aaaacggttt ataaccaacc ggcgaatatg tttgttgccg gatttatagg
     9661 atcaccggcg atgaatttta ttcgcggcac gattgatggc gataaattcg ttacggaaac
     9721 gcttaaatta accatttccg aggagaaatt agcggttctg aaaacacagg aaagtttgca
     9781 taagcccatt gtgatgggaa tacgcccgga agatattcat ccggacgcgc aagaggaaaa
     9841 taacatttcc gccaaaatta gcgtggcaga attaaccggt gctgaattta tgctctacac
     9901 cacggttggg gggcacgaac tggtggtccg tgctggtgcg ttaaatgatt atcatgcagg
     9961 agaaaatatc actattcatt ttgatatgac taaatgtcat ttctttgatg cagaaacgga
    10021 aatagcaatt cgctaaatac agggggaagg cattccccca ggataataca aggaacaata
    10081 atgaaaaaat tattaccctg taccgcactg gtgatgtgtg cgggaatggc ctgcgcacag
    10141 gccgaggaaa agaacgactg gcactttaat atcggcgcga tgtacgaaat agaaaacgtc
    10201 gagggttatg gcgaagatat ggatgggctg gcggagcctt ctgtctattt taatgccgcc
    10261 aacgggccgt ggagaatttc tctggcctat tatcaggaag ggccggtaga ttatagcgcg
    10321 ggtaaacgtg gaacgtggtt tgatcgcccg gagctggagg tacattatca gttcctcgaa
    10381 agcgatgatt tcagtttcgg tctgactggt ggtttccgta attatggtta tcactacgtt
    10441 gatgaaccgg gtaaagacac ggcgaatatg cagcgctgga aaattgcgcc agactgggat
    10501 gtgaaactga ctgacgattt acgtttcaac ggttggctgt cgatgtataa atttgccaac
    10561 gatctgaata cgaccggtta tgctgatacc cgtgtcgaaa cggaaacggg tctgcaatat
    10621 accttcaacg aaacggttgc cttgcgagtg aactattatc tcgagcgcgg tttcaatatg
    10681 gatgatagcc gcaataacgg tgagttttcc acgcaagaaa ttcgcgccta tttgccgctg
    10741 acgctcggca accactcggt gacgccgtat acgcgcattg ggctggatcg ctggagtaac
    10801 tgggactggc aggatgatat tgaacgtgaa ggccatgatt ttaaccgtgt ggggttattt
    10861 tacggttatg atttccagaa tggtctttcc gtttcgctgg aatacgcgtt tgagtggcag
    10921 gatcacgacg aaggcgacag cgataaattc cattatgcag gtgtcggcgt aaattactcg
    10981 ttctgataat gggttaaatt gccggatgcg gcgcgagcgc caaatccggc atacatttat
    11041 ttcaacgaca tatgcgtggc aacggtaata ttttgtggtg acggttttcc ggcaattaag
    11101 cggaataata actcgcagct ttgttgccct aactcctgcg tcggaacatc gatgccgccc
    11161 ggtgcaggcg ttaaaataaa tgacagcgtt tcattgctat agcccaccac cgctaactgc
    11221 tgcggaatag caatattttt ctctgctgcc gcacgataaa tgctcattaa tttcaggctg
    11281 tcagtggcaa atacggcttc tggcaacggc gactggctta ataattgccg tgcggcttgc
    11341 aaggctgttt catgggtata accgccgtca acaatccatt catcacgcac tgcaatatta
    11401 tgcgcagcca gactttgctt atagccatta acgcggtcaa ccgagacatg gacatcaagc
    11461 ggagcatgca ggcaggcaat attttgattc ccactttcaa ttaacgcatc ggtcaacgca
    11521 atgctgtcgc caaaattatc ggtatcgaca gaataaacat gggcatattg accttcaact
    11581 ttgccaatta ccaccaccgg aatatcatat ttatcgagtt gggcaaaaaa tgactcatcc
    11641 gctggcgaac ttagcataat aatgccttta atcattttct gcttaatttt gctttcgcat
    11701 ttttgtaagt cttctgccag gttgtgcgac gtctgcaata tcacgtcgaa accttcttct
    11761 tcagctttgg cggtgatggc atgtaaaact tccgagaaaa acggattacc cgccgtagtt
    11821 ttggtcgaac gggtagaaat caccataatg gcatcaaaac ccgaagaggt cagcgcgcgt
    11881 gccagcttgt ttggttgata ctgtaattct tcaatggccc gtaacacttt ttcgcgcgct
    11941 tccggggaga tattggtttg cttattcagc acgcgtgata cggtggattt tgatacgcct
    12001 gcaaccctgg caatatcata aatagtaggg gacataggtc aggaactccg tccgaacgtg
    12061 aatgttgcac atcttatgga gttcgcccgt cgatgacaac acgggcagtt gataatcaat
    12121 ggcctggccc ccacattcat atccttacga atgatttttt tctggtcttc agagcagcac
    12181 aggacagcaa tgaagcgact taaaaatgaa cttaatgcgc tggtgaatcg gggtgtcgac
    12241 agacatctgc gcctcgctgt aaccggactt agccgcagcg gcaaaacagc gtttatcacc
    12301 gcgatggtta atcagttgct caatattcat gccggagcac gtttgccgct attaagtgcg
    12361 gtgcgtgaag aacgcctgct gggcgtaaaa cgcattcccc agcgtgactt tggcattccg
    12421 cgcttcacct atgatgaagg actggcgcag ttatatggcg atcctcccgc ctggccgacg
    12481 ccaacgcgcg gcgtcagcga aattcgcctg gcgctacgct ataaatcgaa cgattcgctg
    12541 ctgcgccact ttaaggatac ctccacgctg tatttggaga ttgtggatta ccccggcgaa
    12601 tggttgctcg acctgccgat gctggcgcag gactatttaa gctggtcgcg ccagatgacg
    12661 ggcttactca atggtcagcg cggcgaatgg tcggcgaaat ggcgaatgat gtgcgaaggg
    12721 ctggacccgc tagcgcctgc cgacgaaaac cggctggcag acattgccgc cgcgtggacc
    12781 gattatctcc accactgtaa acagcagggg ctgcacttta ttcagcctgg gcgctttgtc
    12841 ttgccgggag atatggcagg tgcgcccgcg ctgcaattct tcccatggcc agatgtcgat
    12901 gcctggggcg agtctaaact ggcgcaggcc gataaacaca ccaacgccgg aatgttgcgc
    12961 gagcgtttta attattactg cgagaaggtg gtgaaaggat tctataaaaa tcacttcctg
    13021 cgttttgacc gccagattgt gctggtggac tgcctgcaac cgctcaacag tggaccacag
    13081 gcatttaatg atatgcgtct ggcgctgacg cagctgatgc aaagtttcca ctacgggcag
    13141 cgtaccctgt tccggcgttt gttttcgccg gttatcgata agctattgtt tgctgccact
    13201 aaagcggacc atgtgaccat cgatcagcac gctaatatgg tttcattact gcaacaactg
    13261 attcaggatg cctggcaaaa tgcagcgttc gaagggatca gcatggactg cctggggctg
    13321 gcgtcagttc aggcgaccac cagcggcatt attgatgtta acggtgagaa aatcccggcg
    13381 ctgcgcggta atcgacttag tgatggcgca ccgctcactg tttatcctgg cgaagtcccc
    13441 gcacgtttgc ctggtcaggc gttctgggat aagcaaggct tccagtttga ggcgtttcgt
    13501 ccgcaggtga tggatgtcga caaaccgcta ccgcatattc gccttgatgc tgcgctggaa
    13561 tttttaatag gagataaatt gcgatgaccg aaccgttaaa accacgtatt gatttcgacg
    13621 gtccactgga ggtcgatcag aatccaaaat tcagggcgca gcagaccttt gacgaaaatc
    13681 aggcgcaaaa ttttgccccg gccacgctcg acgaagcgca ggaagaagag gggcaagttg
    13741 aagcggtgat ggacgcagcg ttacgtccga aacgcagcct gtggcgcaaa atggtgatgg
    13801 gcgggttggc tctgtttggc gcaagcgttg tcgggcaggg tgtacagtgg acaatgaacg
    13861 cctggcaaac ccaggactgg gtggcgctgg gtggatgtgc cgctggggca ttgattatcg
    13921 gcgctggcgt aggttctgtg gtaacagagt ggcggcgctt atggcgcttg cgacagcgcg
    13981 cccatgaacg cgacgaagcg cgtgatttat tgcatagtca cggcacgggt aaaggccgcg
    14041 cattttgcga aaaactggca cagcaggcgg gcatcgatca gtcacatccg gcgctgcaac
    14101 gctggtatgc ctcaatccat gaaacgcaaa acgaccgtga agtggtcagt ttgtatgcgc
    14161 atttggtcca gccagtttta gatgcccagg cgcggcgcga aatcagccgt tcggcagcgg
    14221 aatcaacatt gatgattgcg gtcagcccac tggcgctggt ggatatggct tttatcgcct
    14281 ggcgcaatct gcgtttgatc aatcgcatcg ccacgctgta tggcattgaa ctgggatatt
    14341 acagccgttt gcgcctgttt aagctggtat tgctgaatat cgctttcgcc ggagccagcg
    14401 aactggtgcg cgaagtgggg atggactgga tgtcgcaaga tctcgctgcg cgtttgtcta
    14461 cccgggcagc tcaggggatt ggtgctggac ttctgacggc acgactgggg attaaagcta
    14521 tggaactttg ccgcccgctg ccgtggattg aagatgacaa acctcgcctc ggggatttcc
    14581 gtcgtcagct tatcggtcag gtgaaagaga cgctgcaaaa gggcaaaacg cccagcgaaa
    14641 aataatgcaa tatcgggtgc tgaccggata tctttacgcc gaagtgcccg tttttccgtc
    14701 tttgtgtcaa tcattgttga cagaaacctt cctgctatcc aaatagtgtc atatcatcat
    14761 attaattgtt cttttttcag gtgaaggttc ccatgcgtct ggaagtcttt tgtgaagacc
    14821 gacttggtct gacccgcgaa ttactcgatc tactggtgct gagaggcatt gatttacgcg
    14881 gtattgagat tgaccccatt gggcgaatct acctcaattt tgccgaactg gaatttgaaa
    14941 gtttcagcag tctgatggcc gaaatacgcc gtattgcagg tgttaccgat gtgcgcactg
    15001 ttccgtggat gccttccgaa cgtgagcatc tggcgttgag cgcgttactg gaggcgttgc
    15061 ctgagcctgt gctttctgtt gatatgaaaa gcaaagtgga tatggcgaac ccggcgagct
    15121 gtcagctttt tgggcaaaaa ttggatcgcc tgcgcaacca taccgccgcg caattaatta
    15181 acggctttaa ttttttacgc tggctggaaa gcgaaccgca agactcgcat aacgagcatg
    15241 ttgttattaa tgggcagaat ttcctgatgg agattacgcc tgtttatctt caggatgaaa
    15301 atgatcaaca tgttctgacc ggtgcggtgg tgatgttgcg ctcgacgatt cgtatgggcc
    15361 gtcagttgca gaatgtcgct gcacaggacg tcagtgcctt cagtcaaatt gtcgccgtca
    15421 gcccgaaaat gaagcatgtt gtcgaacagg cgcagaaact ggcgatgcta agcgcgccgt
    15481 tgctgattac gggtgacacc ggtacaggta aagatctctt tgcctacgcc tgccatcagg
    15541 caagcccacg agcgggcaaa ccttacctgg cgctgaactg cgcgtctata ccggaagatg
    15601 cggtcgagag tgaactgttt ggtcatgctc cggaaggtaa aaaagggttc tttgagcagg
    15661 cgaacggtgg ttcggtgctg ttagatgaga taggcgagat gtcgccaagg atgcaggcga
    15721 aattactgcg tttcctcaat gatggtactt tccgtcgagt tggtgaagat catgaagtgc
    15781 atgtcgatgt aagggtgatt tgcgctacgc agaagaattt ggtcgaactg gtgcaaaaag
    15841 gcgtgttccg tgaagatctc tattatcgtc tgaacgtgtt gacgctcaat ctgccgccgc
    15901 tacgtgactg tccgcaggac atcatgccgt taaccgagct gttcgtcgcc cgctttgccg
    15961 acgagcaggg cgtgccgcgt ccgaaactgg ccgctgacct gaatactgta cttacacgtt
    16021 atgcgtggcc gggaaatgtg cggcagttaa agaacgctat ctatcgcgca ctgacacaac
    16081 tggacggtta tgagctgcgt ccacaggata ttttgttgcc ggattatgac gccgcaacgg
    16141 tagccgtggg cgaagatgcg atggaaggtt cgctggacga aatcaccagt cgttttgaac
    16201 gctcggtatt aacccagctt tatcgcaatt atcccagcac gcgcaaactg gcaaaacgtc
    16261 tcggcgtttc acataccgcg attgccaata agttgcggga atacggtctg agtcagaaga
    16321 agaacgaaga gtaagcgcga atatgcctga tggtgcaaca ccatcaggca tattaactta
    16381 tgctttcagt acagccagag ctgcttcgta atccggctcg gtggtgattt catccaccag
    16441 ctggctgaaa atcacattgt cattttcatc aataaccaca acggcacgcg ctgccagacc
    16501 tttcagtggg ccatcagcaa ttgccacacc gtaggcttgc agaaattcag cgttacggaa
    16561 agtggagagg gtgataacgt tgttcagacc ttctgcgccg cagaaacgag actgggcgaa
    16621 cggcagatcg gcagagatac acagcacaac ggtgttgtcg atctctgttg ccagttggtt
    16681 aaacttacgt actgatgcgg cgcaaacacc ggtatcaata ctcgggaaaa tgttcagcac
    16741 tttgcgttta cccgcaaact gaccgagggt gacgtcagac agatcttttg ccacgagagt
    16801 aaaagtctgc gctttgctac ccgcctgcgg gatggaattg gcgactgtaa ccgggttgcc
    16861 ctggaaatga acggtttgtg acatgattat ctttcctgtt tacatatagt taacgtcaca
    16921 cctagtttat gctaactgtc aataacacag caaacgctat ttgcgcttaa tccgcagacc
    16981 accgcgacaa caaggagtaa agatgagaac cgttaaggta ttcgaggaag gctggccctt
    17041 acataccccg tttgtgattg ctcggggaag tcgcagtgaa gcgcgcgtgg tggtggttga
    17101 actggaagaa gaaggtatta aaggcaccgg tgaatgcacg ccgtatccgc gttatggaga
    17161 aagtgatgcc tcggtaatgg cgcaaattat gagcgtcgtg ccgcaattag agaaagggct
    17221 gacacgggag gagttgcaaa aaatcctccc tgccggcgca gcacgtaatg cgctggattg
    17281 tgcattgtgg gatctcgcgg cgcgaaaaca gcaggaatcg ctggctgatt tgatcggcat
    17341 aacgcttccc gggacagtta tcactgcaca gacggttgtc atcggtacgc ctgatcagat
    17401 ggccaatagt gcatcaacac tctggcaggc aggcgcgaaa ttactgaaag tgaagctgga
    17461 taaccatctt atcagtgagc ggatggtggc aattcgcacc gctgtgcccg atgcgacgct
    17521 gatcgttgat gcaaatgaat cctggcgtgc agaagggttg gcggcgcgtt gccagctatt
    17581 ggcggattta ggcgttgcaa tgcttgaaca accgcttcct gcgcaggacg atgcggcgtt
    17641 ggagaatttt attcatccgt taccgatttg tgctgatgaa agttgtcata ctcgtagcaa
    17701 tctgaaggcg ctgaaagggc gctatgagat ggttaacatt aagctcgata aaacgggggg
    17761 cctgacggaa gcgctggcgc tggcgactga agcacgtgca caaggtttta gtctgatgct
    17821 gggctgcatg ttgtgtacct ctcgtgccat tagcgccgct ttaccgctgg tgccgcaggt
    17881 cagtttcgcc gatcttgacg gaccgacctg gctggcggta gatgtggaac cggcgcttca
    17941 gttcacaacg ggcgaattgc atctttagga tgccagcgca gcaagtttgc catcgcaaag
    18001 agatattttt cgctggcttc gtcggaggag atgggtggaa actctgcggt gatgcaatgc
    18061 aggttgagat ccgcacacca actgccgaaa gagccaggcg tttcataacc gacgctggtt
    18121 accagaggca attcaaacgc ctgggccagc cattcaccta attcgctgtg tctgggatct
    18181 tcaatacagg ccagcggatc gtggaatgag accacccagg cgggttgaat gcggtgtata
    18241 agctggcaca gcgcctgggt ttcaggttcg gagccaggtt tgtcacctgt cagtaaaacg
    18301 acatcacgtt cttcagcggc gctgttccag cgataaacgg tttcaccttc cttccagttt
    18361 gccgccggaa agtttctgtt taaatccaca ccattagcat tggcccttaa ccccaactgg
    18421 cagccgtcag gattcacaca cagcaccaca tgatgacggc gcaaagaagg tgtcaacgtc
    18481 cgcagagcac aggagagcgt gacgatcgaa gagttttcat caccgtgagt gccagccaga
    18541 atcaaaccac tttcgtgact ggctgcaggg gccggaaacc agattaacgg cgcgcccaat
    18601 aatgaacgtc cgtaatgttc tgttccgggc ggaaatgcgc cgcgttcggc gcgtgggcga
    18661 gttacggtca tgatcgtctc tggttcagga agtattacag gcagtgttgt gcaaattttg
    18721 cggattatca aatccttttc ttcaacgcta tgttatcgat cttgtatcac ttttttgccg
    18781 gaagttgttt gcattttcat tgatgtgcag atattagaat tgtcgtaatg ttatatacat
    18841 ggcgtatttt attcttactg gttctacaca tgttaatcgc atctgattga tcagttcacc
    18901 ttaataagtt taataaatcg tactgcacat agtacagtac gatggtagct atataattga
    18961 tttacatgaa gaatggctta tcgccatcag tcccgggttg attgattcct atattctcac
    19021 caatgtgttt ctcaggttta tcgatgatat ctgtaattaa tgctgcgaca ctcttacgtg
    19081 atacgatcgt acctttgaat ggttcattgc gtgaggtaag ctcataatca ataatatctt
    19141 catcagtcag ccaggcaggg cgaagaattg tatattccag tccagaagcc tcaattgcat
    19201 ctgcagccct gcggaatggt tttaggggtt caccaataac tgcattattc cattcgccaa
    19261 attttcctgg aacttcatca taaataccga gggaaagaac aaagatcaga cgtttaacat
    19321 caccggcttt catcgcagcg atcactgaat ttgcctgaat atctaaatct tcccccgtaa
    19381 ggttggcata cacaacatcc tgtccctgca tggcctgctt cagtgctgca tgattcagta
    19441 catcccccat aataatcttg ctattagtgg gatagggttt gtggattttt gctggctgac
    19501 gggcaaataa agtttgttta atggtttgtt tatctgccag ttgattaatc acatggcggg
    19561 ctatctgacc gccagcacca agaatcagta catttttcat gaaacttttc ccagattatt
    19621 ggcagcaata tgccaggcca tatcactatt acggatttga atatgtttca ttacagagtt
    19681 tcctcaaaga acggagctaa tactgaaatt gcttcagcca catagacttt gccgtcataa
    19741 agatccatat ggtttgcgcc ttcaacaatg tgatagcgtt tatcctggct tgatgctcga
    19801 tcgtacaggt cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgtatc
    19861 ggctgagtca ggtacacttc cgccatatgg taagcatcat aggtaataat ctggttaagg
    19921 ctgcgcaaag tagcgtaacc cggtgctgtt ggatactgcg cgcgaggggt atggtaatac
    19981 tcccaggcct gacgcagttc ttcgttcggc gcatcggact ccttcattgg tgccagtggc
    20041 atagtggcgt attctctgct gctaatatcg ctggttcggg cgtttgaacc cgcgtcaacg
    20101 tatggtaggg catctataga tttcacattg ttttcccaac cattacggaa catcgaacca
    20161 atattgaccg cactaacagt accgatggcc ttgatgcggc gatcctgaat tgcagcattg
    20221 gctgtatatc ctgcaccggc acaaattccc atcgcaccaa ttcggctatt gtcgacataa
    20281 gaaagcgttg tcaggtaatc aatcacggca ctgacgtctt cagtacgaat gtatgggttt
    20341 tctaactgac gcggctcgcc gccgctttca ccttgataag atgcgtcata agcaatagtg
    20401 acaaatccct tttccgccag tttttcggca taggttccag ccgtttgctc tttaacgccc
    20461 ccacctgggt gagataccac aattgccgga tactgacggg tttcatcaaa ttttggaggg
    20521 aaatagatca ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta
    20581 ttattcatca ttccgttcct catggagttg tcggttcgtt ttgacggttg gtgatatcac
    20641 tatagatatt gatcattaag ttgattagac ccaaaatcat gattagacct atcatttaaa
    20701 tgattaatag attgtatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc
    20761 gtttgtcgtc gttgcagagg agcgtagctt cactcgcgca gcagcccgcc tgagcatggc
    20821 gcagtcagct ttaagccaga tagtgcgccg tatagaagaa cgattgggat tgcggcttct
    20881 gacacgaacc acgcgcagcg ttgtacccac tgaagcgggc gagcatcttt tgtctgttct
    20941 tggccctatg ttgcatgaca tagattctgc cctgacatcc ctgagcgatc tgcagaaccg
    21001 cccatccggg acaatacgta ttactactgt agaacatgca gcaaaaacga tattgttacc
    21061 agctatgcgc acattcctga aatcgcatcc tgaaattgat atccagctta ccattgatta
    21121 tggtttgacc gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg gtggggagat
    21181 ggataaagat atgatcgcca ttcgaatcgg gccagatata ccaatggcta ttgttggctc
    21241 accggattat ttttctcgtc gaagtgctcc aacctcagtg tcacaattaa tagatcatca
    21301 ggcaattaat ttgtatcttc ccacatcggg tacagcaaat cgctggagat taatacgcgg
    21361 tggacgtgaa gttcgtgttc gcatggaagg tcagctttta ctgaatacga tagacctgat
    21421 cattgatgct gcaattgatg ggcatggatt ggcgtatcta ccttatgatc aggttgagcg
    21481 ggctattaaa gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag atttacccgg
    21541 ttatcacctg tactatccac accgtcgaca tgctggctcg gcattctcat tatttataga
    21601 taggctgaag tataaaggtg ctgtttagca ctacttgctg atacattaat ttaattcttc
    21661 tcttaacgta ttctcagttc ctttcaacgt tttggttatt ttttattctt cgtacaatgg
    21721 cgacagatgc tgattatgat accgaaaacg ggtttgaacg tgcgaagccc gaa
//
LOCUS       DAZKGF010000082        21554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZKGF010000082 DAZKGF010000000
VERSION     DAZKGF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.082"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2934
                     /locus_tag="ACHOQV_002841"
     CDS             <1..2934
                     /locus_tag="ACHOQV_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP9631186.1"
                     /translation="AAHGTDATSKITNVKDGNLTAGSTDAVNGSQLKTTNDAVAANTT
                     NIATNTTNITNLTDAVDSLGDDSLLWNATAGAFSAKHGTNGTDSKITNLLAGTVSSDS
                     TDAINGSQLYGLADSFTSYLGGGADISDAGVLTGPTYTIGGTDYNNVGDALAAINTSF
                     STSLGDALLWDATAKGGDGAFSAGRGKDNTASIITNVADGAISSTSSDAINGSQLYDT
                     SKYIADTLGGDAEVNADGTITAPTYAIAGGSYSNVGDALEAIDTTLDDALLWDATAND
                     GNGAFSAAHGKDKTASVITNVANGAISATSSDAINGSQLYTTNKYIADALGGDAEVNA
                     DGSITAPTYTIANAEYNNVGDALDALDDNALLWDATANDGAGAYNASHDGKASIITNV
                     ADGNIGEGSTDAINGSQLFNTNMMIQQNSEIINQLAGNTSETYIEDNGAGINYVRTND
                     NGLAFNDASASGIGATAVGYNSVAEGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIVK
                     GSRDTSVSEEGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQN
                     AIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLVLADAIN
                     GIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQI
                     TNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENGIGDIVS
                     TGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVANEENTISVGSST
                     NQRRITNVAAGKNATDAVNVAQLKSSEAGGVRYDTKADGSVDYSNITLGGGNGGTTRI
                     SNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMT
                     GLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALG
                     AGIQW"
     gene            3346..5001
                     /gene="lldP"
                     /locus_tag="ACHOQV_002842"
     CDS             3346..5001
                     /gene="lldP"
                     /locus_tag="ACHOQV_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP9631187.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVIYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            5001..5777
                     /gene="lldR"
                     /locus_tag="ACHOQV_002843"
     CDS             5001..5777
                     /gene="lldR"
                     /locus_tag="ACHOQV_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFP9631188.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            5774..6964
                     /gene="lldD"
                     /locus_tag="ACHOQV_002844"
     CDS             5774..6964
                     /gene="lldD"
                     /locus_tag="ACHOQV_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFP9631189.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIREFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            7037..7510
                     /gene="trmL"
                     /locus_tag="ACHOQV_002845"
     CDS             7037..7510
                     /gene="trmL"
                     /locus_tag="ACHOQV_002845"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP9631190.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPTEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(7563..8384)
                     /gene="cysE"
                     /locus_tag="ACHOQV_002846"
     CDS             complement(7563..8384)
                     /gene="cysE"
                     /locus_tag="ACHOQV_002846"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFP9631191.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8464..9483)
                     /gene="gpsA"
                     /locus_tag="ACHOQV_002847"
     CDS             complement(8464..9483)
                     /gene="gpsA"
                     /locus_tag="ACHOQV_002847"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9631192.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9483..9950)
                     /gene="secB"
                     /locus_tag="ACHOQV_002848"
     CDS             complement(9483..9950)
                     /gene="secB"
                     /locus_tag="ACHOQV_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFP9631193.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(10013..10264)
                     /gene="grxC"
                     /locus_tag="ACHOQV_002849"
     CDS             complement(10013..10264)
                     /gene="grxC"
                     /locus_tag="ACHOQV_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFP9631194.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10406..10837)
                     /locus_tag="ACHOQV_002850"
     CDS             complement(10406..10837)
                     /locus_tag="ACHOQV_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9631195.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVIVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            11082..12626
                     /gene="gpmM"
                     /locus_tag="ACHOQV_002851"
                     /gene_synonym="pgmI"
     CDS             11082..12626
                     /gene="gpmM"
                     /locus_tag="ACHOQV_002851"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFP9631196.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12660..13919
                     /gene="envC"
                     /locus_tag="ACHOQV_002852"
     CDS             12660..13919
                     /gene="envC"
                     /locus_tag="ACHOQV_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFP9631197.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13923..14882
                     /locus_tag="ACHOQV_002853"
     CDS             13923..14882
                     /locus_tag="ACHOQV_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFP9631198.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(14869..15903)
                     /gene="waaH"
                     /locus_tag="ACHOQV_002854"
     CDS             complement(14869..15903)
                     /gene="waaH"
                     /locus_tag="ACHOQV_002854"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFP9631199.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(16142..17167)
                     /gene="tdh"
                     /locus_tag="ACHOQV_002855"
     CDS             complement(16142..17167)
                     /gene="tdh"
                     /locus_tag="ACHOQV_002855"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP9631200.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARYVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(17177..18373)
                     /gene="kbl"
                     /locus_tag="ACHOQV_002856"
     CDS             complement(17177..18373)
                     /gene="kbl"
                     /locus_tag="ACHOQV_002856"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP9631201.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            18587..19519
                     /gene="rfaD"
                     /locus_tag="ACHOQV_002857"
     CDS             18587..19519
                     /gene="rfaD"
                     /locus_tag="ACHOQV_002857"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFP9631202.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            19529..20575
                     /gene="rfaF"
                     /locus_tag="ACHOQV_002858"
     CDS             19529..20575
                     /gene="rfaF"
                     /locus_tag="ACHOQV_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFP9631203.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            20579..>21554
                     /gene="rfaC"
                     /locus_tag="ACHOQV_002859"
     CDS             20579..>21554
                     /gene="rfaC"
                     /locus_tag="ACHOQV_002859"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFP9631204.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKQFIEENAEKAAM"
BASE COUNT         5246 a         5722 c         5650 g         4936 t
ORIGIN      
        1 gccgcgcacg gtactgatgc caccagcaaa atcactaacg tcaaagacgg taacctgacg
       61 gctggcagca ctgacgcggt taacggctct cagctcaaaa ccactaacga tgccgtggca
      121 gccaacacca ccaatatcgc cacgaacacc accaacatca ccaacctgac tgacgctgtt
      181 gacagtctcg gtgacgattc cctgctgtgg aacgctacgg cgggtgcatt cagtgccaaa
      241 cacggcacca acggtactga cagcaaaatc accaacttac tggcaggcac tgtatcctct
      301 gatagcactg acgccattaa tggctcacaa ctttatggtt tagctgattc atttacgtca
      361 taccttggcg gtggtgctga tatcagcgat gcgggtgtat taaccgggcc aacctacact
      421 attggtggta ctgactacaa taacgtcggt gatgctctgg cagccattaa cacgtcattt
      481 agcacatcac tcggtgacgc cctactctgg gatgcgaccg ccaagggcgg tgacggtgcg
      541 tttagcgccg gtcgcggaaa agataacacg gccagcatca ttactaatgt tgcagacggt
      601 gcaatttcct ctaccagcag cgacgctatt aacggctcac agctctatga caccagtaag
      661 tacattgccg atactctggg tggtgacgca gaagtcaatg ctgacggcac aatcaccgca
      721 ccgacttatg ctatcgctgg cggcagttac agcaacgtcg gtgacgcgct ggaagcaatc
      781 gataccacgc tcgatgacgc tctgttgtgg gatgccacag ccaatgatgg taatggtgca
      841 tttagcgccg ctcacggaaa agataaaaca gccagtgtaa tcactaacgt cgctaacggt
      901 gcaatctctg ccaccagcag cgatgccatt aacggttcac aactgtatac caccaacaag
      961 tacattgccg atgccctggg tggtgacgca gaagttaacg ctgatggttc tatcactgcg
     1021 ccgacttaca ccattgcaaa tgccgagtac aacaacgtcg gtgacgccct ggatgcgctc
     1081 gacgataacg ctctgctgtg ggatgcaacg gccaatgacg gcgcaggtgc ctacaacgcc
     1141 agccatgacg gcaaggccag catcatcaca aatgttgctg atggtaacat tggcgaaggc
     1201 agtaccgacg ccatcaacgg ttctcagctg tttaacacca atatgatgat ccagcagaac
     1261 agcgaaatca tcaatcagct tgccggtaac accagcgaaa cctacatcga agataacggt
     1321 gcgggtatta actatgtacg taccaacgac aacggcttag cgttcaacga tgccagcgct
     1381 tcaggtattg gcgctacggc tgtgggttat aactctgttg ccgaaggcga tagcagcgta
     1441 gccatcggtc agggcagcta cagcgacgtt gataccggta ttgccctggg tagcagctcc
     1501 gtttccagcc gtgtaatagt taaaggttct cgtgacacca gcgtgtcgga agaaggtgtt
     1561 gtgattggtt atgacaccac ggatggcgaa ctacttggtg cgctgtctat tggtgatgac
     1621 ggtaaatatc gtcaaatcat caacgtagcc gatggttccg aagcccatga cgccgttacg
     1681 gttcgccagt tgcaaaatgc cattggtgca gtcgctacca cgccgaccaa atacttccac
     1741 gccaactcaa cggctgaaga ctcactggct gtcggggaag actcgctggc gatgggcgcg
     1801 aaaactatcg tcaatggtaa tgcgggtatt ggtatcggcc tgaatactct ggttctggca
     1861 gatgccatca acggtattgc tatcggttct aacgcacgcg ccaaccatgc aaacagtatt
     1921 gcaatgggta atggttctca gaccactcgc ggcgctcaga ctggctacac cgcctacaac
     1981 atggatgcac cgcagaactc tgtgggtgaa ttctccgtag gtagcgaaga cggtcaacgt
     2041 cagatcacca acgtcgcagc ggggtcggcg gataccgatg cggttaacgt gggtcagttg
     2101 aaagtaacgg acgcgcaggt ttcccagaat acccagagca ttactaacct gaatactcag
     2161 gtaacgaatc tggatacccg cgtcaccaat atcgaaaacg gtattggcga tatcgtaagc
     2221 accggtagca ccaagtactt caagaccaac accgatggcg tagatgctaa cgcgcagggt
     2281 aaagacagtg ttgcgattgg ctctggctct attgctgccg ctgataacag cgttgcgctg
     2341 ggtactggct ctgtggcaaa cgaagaaaat acgatctccg taggttcttc cactaaccaa
     2401 cgccgtatca ccaacgttgc cgctggtaaa aatgctactg acgcggttaa cgttgcgcag
     2461 ttgaagtctt ccgaagcagg cggcgtgcgg tatgacacca aagctgacgg ttctgttgat
     2521 tacagcaaca tcactctcgg cggtggtaat ggcggaacga ctcgcatcag caacgtttcc
     2581 gctggcgtta acaacaacga cgcggtgaac tacgcgcagt tgaagcaaag cgtgcaggaa
     2641 acgaagcaat acaccgatca gcgcatggtt gagatggata acaaactgtc caaaaccgaa
     2701 agcaagttga gcggtggtat cgcttctgca atggcgatga ccggtctgcc gcaggcttac
     2761 acgccaggtg ccagcatggc ctctattggt ggcggtactt acaacggtga atcggctgtt
     2821 gctttaggtg tgtcgatggt gagcgccaat ggtcgttggg tctacaaatt acaaggtagt
     2881 accaatagcc agggtgaata ctccgccgcg ctcggtgccg gtattcagtg gtaatcatcc
     2941 attaacgaat gggtaaacgc cacattgcct gatgcgctac gcttatcagg cctacggggt
     3001 gattcagcac taccctgtag gccggataag gcgttcacgc cgcatccggc aagataaggc
     3061 gcaccagatc aacaatctga gggcaattct ctgatgggaa ttgccctttt cttcattacc
     3121 tctctcccag aattggccct accaattctt cattgatctg acctctggtt cacaattccc
     3181 caattaaaac tcacatcacc gttgccaata cataacatct agttaactat tcattgtcat
     3241 tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca aggagatgag
     3301 caacagactc attacacggc gtgtgtggac tgcaggagac ctgcaatgaa tctctggcaa
     3361 caaaactacg atcccgccgg gaatatctgg ctttccagcc tgatagcatc gcttcccatc
     3421 ctgtttttct tttttgcgct gattaagctc aaactgaaag gatacgtcgc cgcctcatgg
     3481 acggtggcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt cgctaacgcg
     3541 ctggcctcgg tgatttatgg cttcttctac ggtttgtggc ccatcgcgtg gatcattatt
     3601 gcggcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat catccgctcg
     3661 tctattcttt cgataacccc tgaccagcgc ctgcaaatgc tgatcgtcgg tttctgtttc
     3721 ggcgctttcc ttgaaggagc cgcaggcttt ggcgctccgg tagcaattac cgccgcattg
     3781 ctggtcggtc tgggctttaa accactctac gccgccggac tgtgcctgat tgttaacacc
     3841 gcgccagtgg catttggtgc gatgggtatt ccgattctgg tcgccgggca ggtaacaggc
     3901 atcgacagct ttgagattgg tcagatggtg gggcgtcagt tgccgtttat gaccattatc
     3961 gtgctgttct ggatcatggc gattatggac ggttggcgcg gaatcaaaga aacgtggcct
     4021 gcggtcgtgg tggcaggtgg ttcgtttgcc attgctcaat acctcagctc taacttcatt
     4081 gggccggaac tgccagacat tatctcttcg ctggtatcac tgctctgcct gacgctgttc
     4141 ctcaaacgct ggcaaccagt gcgcgtattc cgtttcggtg acttgggggc gtcacaggtt
     4201 gatatgacgc tggctcacac cggttacacc gcaggtcagg tgcttcgcgc ctggacaccg
     4261 ttcctgttcc tgaccgccac cgtaacgctg tggagtatcc cgccgtttaa agccctgttc
     4321 gcttcaggcg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta cctcgataaa
     4381 ctggttgccc gtatgccgcc agtggtcagc gaagcgacag cgtatgccgc cgtgtttaag
     4441 tttgactggt tctctgccac cggtaccgcc attctttttg ctgccctgct ctcaattgtc
     4501 tggctgaaga tgaaaccatc tgacgctatc agcaccttcg gcagcacgct gaaagaactg
     4561 gctctgccta tctactccat cggtatggtg ctggcgttcg cctttatctc gaactattcc
     4621 ggactatcat caacgctggc gctggcactg gcgcacaccg gccatgcatt caccttcttc
     4681 tcgccgttcc tcggctggct tggtgtcttc ctgaccggat cggatacctc atctaacgcc
     4741 ctgttcgccg ccctgcaagc cactgcagca caacaaattg gcgtttctga cctgttgttg
     4801 gttgccgcca acaccaccgg tggtgtcacc ggtaagatga tctctccgca atctatcgct
     4861 atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc
     4921 aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcttatgtc
     4981 ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg
     5041 atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg
     5101 aacgtcaact ggcgatgcaa ctcggcgtgt cgagaaattc actgcgcgag gcgctggcga
     5161 aactggtcag tgaaggtgtg ctgctcagtc gacgtggcgg cgggacgttt attcgctggc
     5221 gtcatgatac atggtcggag caaaacatcg tccagccgct gaaaacgctg atggccgatg
     5281 atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc agcaccgcgt
     5341 ggcatgcggc aatgcgcgcc acaccaggcg aaaaagagaa aattcagctt tgctttgaag
     5401 caacgctaag tgaagacccg gatctcgcct cacaagcgga cgttcgtttt catctggcga
     5461 ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc
     5521 tgcaatcctc tgtgaagcat agccgccagc ggatgtatct ggtacccccg gttttttcac
     5581 agctgaccga acaacatcag gcagtcattg acgccatttt tgccggtgat gctgacgggg
     5641 cgcgtaaagc aatgatggcg caccttagct ttgttcacac caccatgaaa cgattcgatg
     5701 aagatcaagc tcgccatgca cgcattaccc gcctgcctgg tgaccataat gagcattcga
     5761 gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag cacaacgcat
     5821 tctgccgccg ttcctgttcc actatatgga tgggggtgca tattctgaat acacgctgcg
     5881 ccgcaacgtg gaagatttgt cagaagtggc gctgcgccag cgtattctga aaaacatgtc
     5941 tgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg tggcgctagg
     6001 tccggtaggt ttgtgtggca tgtatgcgcg acgcggcgaa gttcaggctg ccaaagccgc
     6061 ggatgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt
     6121 ggctccagcc attaaacgtc cgatgtggtt ccagctttat gtgctgcgcg atcgcggctt
     6181 tatgcgtaac gcactggagc gagcaaaagc ggcaggctgc tcgacactgg ttttcaccgt
     6241 ggatatgccg acaccgggcg cgcgttaccg tgatgcacac tcgggtatga gcggcccaaa
     6301 tgcggcaatg cgccgttact tgcaggcggt gacgcatccg caatgggcgt gggatgtggg
     6361 cctgaacggt cgtccacatg atttaggtaa tatctcggct tacctcggca aaccgaccgg
     6421 actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct catggaaaga
     6481 ccttgagtgg atccgcgaat tctgggatgg tccgatggtg atcaaaggga tcctcgatcc
     6541 ggaagatgcg cgcgatgcag tacgttttgg tgctgatggg attgtggttt ctaaccacgg
     6601 tggccgccag ctggacggtg tactctcttc cgctcgtgca ttgcccgcta ttgcggatgc
     6661 ggtgaaaggt gatattgcca ttctggcaga tagcggaata cgtaacgggc ttgatgtcgt
     6721 gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc tgtatgcgct
     6781 ggcaacagcg ggccaggcgg gcgtggcaaa tctgctgaat ctgatcgaaa aagagatgaa
     6841 agtggcaatg acgctgactg gcgcgaaatc gattagcgaa attacgcaag attcgctggt
     6901 gcaggggctg ggtaaagagt tgccggcggc actggctccg atggcgaaag ggaatgcggc
     6961 atagttgggg gtgaggggaa aaggccgtac caatcaccaa ttttctgcta tcctgccccc
     7021 gcactaaggg ggcagtatgc taaacatcgt actttacgaa ccagaaattc cgccaaatac
     7081 tggcaacatc atccgtcttt gcgctaatac cggctttcgt ctgcatatca tcgaaccaat
     7141 gggatttgcc tgggacgata agcgcctgcg ccgcgcgggg ctggactatc acgagtttac
     7201 cgccgttacg cgtcatcatg actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct
     7261 gtttgccctc accacgaaag gtacgcctgc tcacagcgcc gtaagctatc aggatggcga
     7321 ttatctgatg ttcggcccgg aaacacgcgg cctgccagcg agcattcttg atgccctgcc
     7381 cactgaacaa aaaattcgta ttccaatggt gccggacagc cgcagcatga acctgtctaa
     7441 tgcggtgtcg gtcgtggtgt atgaagcctg gcggcagttg gggtatccgg gagcggtgtt
     7501 gagagattag ttactgtagg ccggatagat gattacatcg catccggcac gatcacagga
     7561 catcagatcc catcaccata ctcaaacgta tggttaatgc cgttgaaatg ctgatccata
     7621 tccattgacg gtttatcgct atccggcttg ccgacaatgc gtgccggaac gccagcggca
     7681 gtggtgtgcg gcggcaccgg ttgcagcacc acggaacctg cgccaatctt cgcgccgcgc
     7741 ccaacttcaa tattaccgag aattttcgcg cccgcgccaa tcatcacgcc ttcacgaatt
     7801 ttcgggtgac ggtcaccacc agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc
     7861 gatacgtcgt tttcaatcac cgccgtttca cccacaacga tgcccgtcgc gtggtcgagc
     7921 atgataccgc gaccaatttt tgctgccggg tgaatatcaa cctggaacgt tacagagacc
     7981 tggttttgta gaaaaatagc cagcgcgcga cggccctgat tccacaacca gtgaccgatg
     8041 cgatacgcct gcaaagcatg aaaacccttc aggtatagca acggggttga gtatttatcc
     8101 accgccgggt cacgggtacg caccgcctga atatcacagg ccgcagaggc gatcatttcc
     8161 gggtcggcgg cgtaggcttc ttccaccact tcacggatag caatagccgg cataatgggc
     8221 gatgacagct tgttcgccag catgtagctc agtgcactgc caaggttttc gtgcttgagt
     8281 agcgtcgcgt ggtaaaaact ggccagcatt ggctcacagt ctgccagcgt tctggcttca
     8341 gctttaatat tgttccagac aatttccagt tcttcacacg acattgctta ctccacacga
     8401 taagataatg accggcccgt tctgcgcggg ccgggtcata gtggtaacaa aggttccctg
     8461 ggtttagtgg ctgctgcgct cgtccttgcg tgcacgacct agtaaagtca atgctgcctc
     8521 gcgcgcgttt tttccgcaat ataatacttg ataaatttcc tcggttattg gcatttcaac
     8581 gccgaagcga tgcgccagtt cgcggacttc tttcgtattg cggtagcctt ccaccacttg
     8641 accaattttc tcctgcgcgc tttgtacatc catgccctga ccgagcatca tgccaaaacg
     8701 gcggttacgc gactggttgt cggtacaggt cagcaccaga tcgcccagcc ccgccatgcc
     8761 cataaaggtg gcaggatcgg cacccagcgc cgcgccaaga cgcgacatct cagccagccc
     8821 acgggtgatc agcgccgtac gcgcattcgc gccaaaaccg ataccgtcgg acatccccgc
     8881 gccaatggca atgacgtttt tcaccgcgcc gccaagctgc acaccgatga aatccgggtt
     8941 gctgtaaacg cggaaacttt tgccacagtg cagcaattgc tggaggtcat cggcaaaggt
     9001 ctggtccgtc gaggccagcg aaatggctgt cggtaaacct gccgccagtt ctttcgcaaa
     9061 cgttgggcca gagataaccg ccagcggaat ttgatcgcct aaggcctcac gcgctacgtc
     9121 ctgtaacaga cgtccggttt ctgcttccag ccctttggtc gcccacacca gacgcgcatc
     9181 aggacgcatc agcggtttaa tctggcgcag cacttcacca aagacatggc tgggtacgac
     9241 gacgagaata ttacggctgg ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt
     9301 atcgggaaaa ggcacatcgg ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc
     9361 gatatgttca gggtcatggc cccagaggac aacctcgtgg ccatttcttg ccagggtaat
     9421 ggcaagagcg gtgccgtacg agccggcacc gatcacagtc attgaagcat tacgttggtt
     9481 catcaggcat cctgatgttc ttcagtacct tcgccagcct gctgctgcaa atagttcatg
     9541 aacagcgcat cgaagttaac cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc
     9601 atgctggtga tgcactcacg agcatacggg aacagaatgt tcgggcagta tgcacccagg
     9661 caatgtgcca tctgggtgcc ttcgataccc gcgatggaga aaataccgcc ctgctgaact
     9721 tcacacagga acgcggtttc ttcgcccagg gaggccgtta cggtaacacg cagtaccact
     9781 tcgtaaacgt catctgccag ttgagaagat gccgtatcca gatcaagttt aacttctggc
     9841 tgccaatctt tctggaaaac gtgcggcgcg ttcggcgctt cgaaagaaat atccttggta
     9901 taaatacgtt ggatctggaa agtcatttcg gtgttgtttt gttctgacat gtgtagaaaa
     9961 cccttaagtg ttgtccttaa atactgcgca atgtaatgcc aacgttcaca cgttatttca
    10021 gcaggggatc cagtccacca cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt
    10081 gctgtgcgtc aataaaaatc tggggaaccg tggtgcgacc gctgcgtttg atcatctctt
    10141 cacgcttagc ggcgttgcca tcgatcggca actcctgaaa actcacgccc ttgctgctca
    10201 gcagtgcttt cgcacgatgg caatacgggc aggtttcttt ggtatagatt tcaacattgg
    10261 ccataactta gctcctgttt ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat
    10321 ggagtgtccc cagaagctta taacaataag tcgctggggg cctaattgca gctaacggcc
    10381 tgcatcatga aagacgacag gtaaattatt tgccgcgcac cagaggtaag ttttcgccag
    10441 cccagccagc aacgccctct ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg
    10501 cgtttgcagg ctcctggcac tgcatgccag aaccgtctac cacgataacc ggtttgtctt
    10561 tgtgcttctc aagctcacca acattgttgg ctttgatttc gctcggcaac aggttgatag
    10621 aacctgcgat atggccttta cggaagtcat cacgctgacg taaatccaca accacagcat
    10681 cttctttgtt gatcagacgc gtagcttcac cacgagtaat caccttcact ttcgaggtca
    10741 ggctcttaaa cgtagtcaca ataaccgcca ccagtaacgc gatccaggcg atactcagta
    10801 tgggatgacg gccaacaaat tgcataattt cttgcatggg gggtaacaac tcccgacgta
    10861 gtgattaaaa aaaccaggaa aggagtatac ctgcgcggtg cagcaaatac agccagcgcg
    10921 ttaactggaa tgcaattttg cggggcgcga cgaaaaaaaa gcgcaaatct ggcatcggct
    10981 tgctgcgagc ggtcgtatat tttgatcttc agaggctatt ttatcgattc agctgtagta
    11041 aaattacgca aattttgact cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac
    11101 ctatggtact ggtgattctg gatggctatg gctatcgtga agaacagcag gataacgcca
    11161 tttttagtgc taaaaccccg gtaatggatg cactgtgggc caatcgtccg cataccctga
    11221 tcgacgcttc cggtctggaa gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag
    11281 gtcacgttaa cctgggtgcc ggccgcatcg tgtatcagga cctgactcgt ctggacgttg
    11341 aaatcaaaga tcgcgctttc ttcgctaacc cggtactgac tggcgcggta gataaagcga
    11401 aaaacgcagg caaagcagtt cacattatgg gtctgctctc cgcaggtggt gtacacagcc
    11461 acgaagatca catcatggcg atggtagaac tggcagctga acgcggcgca gaaaaaatct
    11521 acctgcacgc attccttgac ggtcgcgaca ctccgccgcg cagtgctgaa tcctcgctga
    11581 aaaaattcga agagaaattt gccgcactgg gcaaaggccg cgtagcgtcc atcattggtc
    11641 gttactacgc catggaccgc gataaccgtt gggatcgcgt tgaaaaagct tatgacctgc
    11701 tgactctggc gcagggcgag ttccaggccg ataccgccgt tgctggtttg caggctgctt
    11761 atgctcgcga cgaaaacgac gaattcgtga aagcgaccgt tattcgtgca gaaggtcagc
    11821 cagacgcggc aatggaagac ggcgatgcgc tgattttcat gaacttccgt gctgaccgtg
    11881 cacgtgaaat cactcgtgca ttcgtaaatg ccgattttga cggcttcgcg cgtaagaaag
    11941 tggttaacgt cgatttcgtg atgctgaccg aatacgctgc cgacatcaaa accgcagtgg
    12001 cttacccacc cgcttccctg gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa
    12061 ctcagctgcg tatttccgaa accgaaaaat atgcccacgt tactttcttc ttcaacggtg
    12121 gcgtagaaga gtcgttcaaa ggcgaagatc gcattctgat caactcaccg aaagtggcta
    12181 cctacgatct gcaaccggaa atgagctccg cagagctgac cgaaaaactg gttgcggcaa
    12241 tcaagagcgg caaatacgac accattatct gtaactatcc gaacggcgac atggtaggtc
    12301 acaccggagt gatggaagcg gcggttaaag cggttgaagc gctggatcac tgcgtggaag
    12361 aagtcgcgaa agcggttgag tccgtgggtg gacaactgct gatcaccgct gaccacggta
    12421 acgctgagca gatgcgcgat ccggcaacgg gtcaggcaca cacggcacat accaacctgc
    12481 cagttccgct gatttacgtt ggcgataaga acgtgaaagc ggttgaaggc ggcaaacttt
    12541 ctgatatcgc gccgaccatg ttgtcgctga tgggtatgga aatcccgcaa gagatgactg
    12601 gtaagccgct gttcatcgtg gaataatccc tccccatgag gggaaaggcg attaatacca
    12661 tgacacgggc cgtgaaaccg cgcaggtttg caatcaggcc catcatctac gccagcgttc
    12721 ttagcgctgg cgtattgttg tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca
    12781 aatctattca ggccgatatc gccgcgaaag agcgcgcggt acgccaaaag caacaacaac
    12841 gcgcaagcct gctcgcacaa ttgaaaaagc aggaagaagc gatctcggaa gccacccgta
    12901 agctgcgtga aacgcaaaac acgctcaatc aactgaataa acagattgat gagatgaacg
    12961 cgtcgattgc caaactggag cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac
    13021 tagatgccgc attccgtcag ggcgagcata ccggtattca gctgattctc agcggtgaag
    13081 aaagccagcg tggacagcgt ttacaggctt atttcggcta tctcaaccag gcgcgacaag
    13141 aaaccattgc tcagttgaaa caaacgcgtg aagaagtcgc tatgcaacgc gccgagctgg
    13201 aagagaaaca gagcgagcaa caaacgctgt tgtatgagca gcgcgcccaa caggcgaagc
    13261 tgacccaggc gctgaacgag cgtaaaaaga cgctggcagg gctggagtct tccatccagc
    13321 aaggtcagca acagttgagc gagctgcgcg ccaacgaatc ccgtctgcgt aacagcattg
    13381 cccgtgcgga agccgcggcg aaagcgcgtg ctgaacgaga agcgcgtgag gcccaggcgg
    13441 ttcgcgaccg ccagaaagaa gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg
    13501 aaaaatcgct gatgtcccga actggtggcc tgggtgcgcc gcgtggtcag gcattctggc
    13561 cagttcgcgg gccgacgctg catcgctatg gcgagcagct acagggtgaa ttacgctgga
    13621 aaggaatggt tatcggtgcc tctgaaggta ctgaagttaa agccattgcc gacggtcggg
    13681 tgattctggc tgactggctg caaggctacg gtctggtggt ggtggttgag catggtaaag
    13741 gcgacatgag tctttacggc tataatcaga gcgcactggt gagcgttggt tcgcaggttc
    13801 gcgcgggcca gccgattgca ctggtgggca gcagtggcgg tcagggtcgg ccttcactct
    13861 atttcgaaat tcgccgccag ggtcaggcgg tcaatccaca gccgtggttg ggaagataag
    13921 ttttgtttcc atttcgtcgt aacgttcttg catttgccgc tctgttggcg ctctcctccc
    13981 ccgtaattgc tggcaagctt gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg
    14041 aaaaccaggt gctggcgatg ccttccgcta tctccgtcgc tgtattaccc gattcaccgc
    14101 acgccagaga gatggcaacc aaagcgcata acagcggtca cgaagtgttg attcatctac
    14161 cgatggcacc actgagtaaa cagccgctgg agaaaaatac gctacgcccg gagatgagca
    14221 gcgacgaaat tgagcgcatt attcgtagtg cggtcaataa cgtgccctat gccgtgggga
    14281 tcaacaacca catgggcagc aagatgacct ctaacctgtt tggtatgcag aaagtgatgc
    14341 aggcgctgga gcgttacaat ctttacttcc tcgacagcgt aaccatcggt aatacccagg
    14401 cgatgcgcgc agcgcaaggc actggcgtga aggtgatcaa acggaaagtg ttcctcgacg
    14461 attcgcaaaa tgaagcggac atccgtgtgc agttcaatcg cgcaattgat ctggcgcgtc
    14521 gcaacggttc aaccattgcc attgggcatc ctcacccttc aacggtacgc gtgctacaac
    14581 agatggttta taacctgccg ccagacatta cgctggtgaa agccagcagc ttgctgaatg
    14641 aaccgcaggt tgatacgtct acaccgccga aaaacactgt gcccgacaca ccgcgtaatc
    14701 cgttccgtgg cgtgaagctc tgcaaaccga agaaaccgat agaacctgtt tatgctaatc
    14761 gcttctttga agtattaagc gaaagcatca gccagagcac gctgatcgtt tacttccaac
    14821 atcagtggca aggctggggc aaacagcctg aagcggcaaa acttaacgct agcgtaaatt
    14881 aaaggcgcta cgcgtaatgc gatggtgcga ccgcgttttg tcgcgccatt gccataagcg
    14941 gaaagaccac agtaacgcct gataacccac cttcacgctg cgcacattgg taatcaggcg
    15001 cttatacata ccggaagtaa atatctcggc aatcatccgt tggcgggtaa taatatccgg
    15061 ctctttgcgc accgcatggc aaacacgcaa tgcttcgtaa gttatttgct gatgaaattc
    15121 cggataaatc gtaattttgt cggcataatt tcgatttaat ttctctagca ggcgggtaat
    15181 cttaatatag tgacgttgat aattaaggtt tttattccct tgtctattca accgactcac
    15241 tgacgtatta tgcagataat atttatataa cgattgctcg gtatatcgcg cacgcagcgc
    15301 attaaacata aattctgttg tccagacaat atcctgatga tgtaatccgg caataaattt
    15361 aatgttattt ttaacaataa catcgcggcg ataaaccccc atccagacaa cgtgagtcca
    15421 acggcgggaa gaaagcccca tccgcaacca atccgggccg gttaatacgc cggttgagcg
    15481 aaggcgatcg gtggggatgg attgccaggt ttctcccgtt tcacgaaaac accagtcagc
    15541 gttgcactgc gccacgtcga ggtcgtcctc taacgccatg gtcatcagcg tttcgtacat
    15601 ggtgggatag acctcatcgt cagcatcgac aaaagcgaca tatttgcccg tcgccacctc
    15661 gatcccacga ttacgcgcta ccgatgcgcc agcattcgcc tgatgcaaca aacgaacgtg
    15721 cggatagttt tctgcgtaat gctttgctat ttcaacagaa ttatccgttg aaccatcgtt
    15781 aataataatg atttccagag cagtccaggt ttgcgtaatt aaagattcca tacacgtgcg
    15841 gaaatcatcg cccgcattat ataacgggat aataacacta agtttattgg tgctgttcat
    15901 cataaattac cgacgacctc tgaagttatc ctgacgtttt acaggctttg tattaagaaa
    15961 ctattaagcc tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat
    16021 aatgagaaca gctatataag agatcagcgc aattacgaac tctttattac actgacagga
    16081 ttttacatat ttaacggatt atcctcagga aaaagggctg gaataccagc ccttgtttgt
    16141 gttaatccca gctcaggata actttcccgg actggcccga acgcatggcg tcaaagccct
    16201 tctggaaatc atcgatagag aaacgatgag taatgatcgg cgaaagatcg aggccagact
    16261 gaatcagcgc cgccatcttg taccaggttt caaacatctc acgaccgtaa atacctttaa
    16321 tgaacaagcc tttaaagatc actttggtcc agtctatcga catgtcagaa ggtggaatac
    16381 ccagcatcgc aatacgaccg ccgtggttca tggtgtcaag catggtacga aacgctggcg
    16441 gcgcaccgga catttccaga ccgacatcaa agccttcggt catgcccagt tctgccatca
    16501 catcattgag gttctcttta gcgacgttaa ccgcacgggt gatacccatt ttacgcgcca
    16561 actcaaggcg gtattcgtta acatcagtaa tcaccacgta acgtgcacca acgtgtttcg
    16621 ccaccgccgc cgccataata ccaatcgggc ctgcaccaga gaccagcaca tcctcgccca
    16681 ccagatcgaa cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca
    16741 gatcatcgga gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatact
    16801 cggcgaagca gcccggacgg ttaacgccga cgccgatcgt gttgcggcac agatgggtac
    16861 gaccgccacg acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat
    16921 cgccaatctt gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat
    16981 gacccacgac catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt
    17041 gaacgtcagt cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga
    17101 gttccggtac aggaacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg
    17161 ctttcatctc acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt
    17221 ctactgcacg cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg
    17281 cctgaccttt cggaacgacc ggatagaaga aaccggtgac gtaaatcccc tctttttgca
    17341 gctcacgggc aaatttctgc gccactaccg catcgccaag catgaccgga ataatggcgt
    17401 gatcggctcc cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt
    17461 tcgcccacag acggtcacgc agctcgctac ccgcttcaac catctccagc actttgatgg
    17521 aagcggcaac aatggccggt gccagcgagt tggagaacag gtacggacga gaacgctggc
    17581 gcagccactc aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg
    17641 ctttaccaag cgtaccggtg ataatatcga cccgccccat cacatcgcag tattcatggg
    17701 aaccacgacc gttttcaccg acaaaaccta ccgcgtgtga gtcatctacc atcaccaggg
    17761 catcatattt atctgccaga tcgcaaacgc ctttcaggtt ggcaatcacg ccgtccatcg
    17821 agaacacacc atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca
    17881 gacgtgcttc cagctcctgc atatcgttgt tggcataacg atagcgttta gctttgcaca
    17941 gacgcacacc atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg
    18001 cgcccaacag cgtttcgaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg
    18061 catcttccat ccccaggaag gccgccagtt tttgttcaag ctctttatgg ctgtcctgag
    18121 tgccgcaaat aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg
    18181 ccgccgcaat cagatccgga tgattcgcca gtccgagata gttattggca caaaagttaa
    18241 tgacgtggct tccatcagcc acagtgatat ctgcttgctg cgcagacgta ataatgcgct
    18301 cttctttaaa caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa
    18361 attctccacg cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt
    18421 ataggttgcg acgagatgac gcgttaacac ttctgcaaaa ttcaggataa ataacgtcca
    18481 gccccctcac ttttggtgaa tggctgaagc caacgtcatg tagtgatatg atagaagtat
    18541 tcgtgtctga gattgtctct gactccataa ttcgaaggtt acagttatga tcatcgttac
    18601 cggcggcgcg ggctttatcg gaagcaacat cgttaaagcc ctgaatgata aaggcatcac
    18661 cgatattctg gtggtggaca acctgaaaga cggcaccaag tttgtgaacc tggtggatct
    18721 ggatatcgcg gactatatgg ataaggaaga cttcctgatc cagattatgg ctggcgaaga
    18781 gttcggcgat gtcgaagcga ttttccacga aggcgcgtgc tcttccacca ccgagtggga
    18841 cggcaagtat atgatggata acaactatca atactccaaa gagctgctgc actactgtct
    18901 ggagcgcgaa atcccgttcc tgtatgcctc ttccgcagcc acttacggtg gacgcacctc
    18961 cgactttatt gaatcccgcg aatatgaaaa accgttgaat gtctacggtt actcaaaatt
    19021 cctgtttgat gaatatgttc gccaaatcct gcctgaagcg aactcacaga ttgttggctt
    19081 ccgttatttc aacgtttatg gaccgcgtga aggccataaa ggcagcatgg cgagcgtcgc
    19141 tttccatctc aacacccagc ttaataacgg tgaatcaccg aagctgttcg aaggtagcga
    19201 gaacttcaaa cgtgacttcg tctatgtggg cgatgtggca gatgtaaacc tgtggttcct
    19261 ggaaaatggc gtttccggca tcttcaacct cggtactggt cgtgcggaat ccttccaggc
    19321 agtagcagat gctacgcttg cttatcacaa gaaaggccaa atcgaataca ttccgttccc
    19381 ggataaactg aaaggccgct accaggcgtt cacgcaggca gatctgacaa atctgcgcgc
    19441 ggcgggttac gacaaaccgt ttaaaaccgt tgccgaaggc gtaacggaat acatggcctg
    19501 gctgaatcgt gacgcataag agctctgcat gaaaatactg gtgatcggcc cgtcttgggt
    19561 tggcgacatg atgatgtcgc aaagtctcta tcgcacgctc caggcgcgct atccccaggc
    19621 gataatcgac gtgatggcac cggcatggtg ccgtccatta ttatcgcgga tgccggaagt
    19681 taacgaagct attcctatgc ctctcggtca cggagcgctg gaaatcggcg aacgccgcaa
    19741 actgggtcat agcctgcgcg aaaaacgtta cgaccgcgcc tacgttttac ccaactcctt
    19801 caaatccgca ttagtgcctt tcttcgcggg tattcctcat cgcaccggct ggcgcggcga
    19861 aatgcgctac ggtttactca acgatgtgcg tgtgctggat aaagaagcct ggccgctgat
    19921 ggtggaacgc tatgtggcgc tggcctatga caaaggtatt atgcgtaccg cacaagatct
    19981 gccgcagcca ttgttatggc cgcagttgca ggtgagcgaa ggtgaaaaat catatacctg
    20041 taatcaattt tcgctttcat cagaacgtcc gatgattggt ttttgcccgg gtgcggagtt
    20101 tggtccggca aaacgctggc cacactacca ctatgcggag ctggcaaagc aactgattga
    20161 tgaaggttat caggtggttc tgtttggctc ggcgaaagat catgaagcgg gcaatgagat
    20221 tcttgccgct ttgaataccg agcagcaggc atggtgtcgt aacctggcgg gggaaacaca
    20281 gcttgatcaa gcggttatcc tgattgcagc ctgtaaagcc attgttacta acgattctgg
    20341 cctgatgcac gttgcggcgg cgctcaatcg tccgctggtt gccctgtatg gtccgagtag
    20401 cccggacttc acaccgccgc tatcccataa agcgcgcgtg atccgcctga ttaccggcta
    20461 tcacaaagtg cgtaaaggtg acgctgcgga gggttatcac cagagcttga tcgacattac
    20521 tccccagcgc gtactggaag aactcaacgc gctattgtta caagaggaag cctgacggat
    20581 gcgggttttg atcgttaaaa catcgtcgat gggcgatgtt ctccatacgt tgcccgcact
    20641 cactgatgcc cagcaggcaa tcccagggat taagtttgac tgggtggtgg aagaagggtt
    20701 cgcacagatt ccttcctggc acgccgccgt tgagcgagtt attcctgtgg caatacgtcg
    20761 ctggcgtaaa gcctggttct cggcccccat aaaagctgaa cgcaaagcgt ttcgtgaagc
    20821 gctacaagca gagaactatg acgcagttat cgacgctcag gggctggtaa aaagcgcggc
    20881 gctggtgacg cgtctggcgc atggcgtaaa gcatggcatg gactggcaaa ccgctcgcga
    20941 acctttagcc agcctgtttt acaatcgtaa gcatcatatt gcaaaacagc agcacgccgt
    21001 agaacgcacc cgcgaactgt ttgccaaaag tctgggctac agcaaaccgc aaacccaggg
    21061 cgattatgct atcgcacagc atttcctgac gaacctgcct acagatgctg gcgaatatgc
    21121 cgtatttctt catgcgacga cccgtgatga taaacactgg ccggaagaac actggcgaga
    21181 attgattggt ttactggctg attcaggaat acggattaaa cttccgtggg gcgcgccgca
    21241 tgaggaagaa cgggcgaaac gactggcgga aggatttgct tatgttgaag tattgccgaa
    21301 gatgagtctg gaaggcgttg cccgcgtgct ggccggggct aaatttgtag tgtcggtgga
    21361 tacggggtta agccatttaa cggcggcact ggatagaccc aatatcacgg tttatggacc
    21421 taccgatccg ggattaattg gtgggtatgg gaagaatcag atggtttgta gggctccggg
    21481 gaatgagttg tctcaattga cagcaaatgc tgttaagcaa ttcattgaag aaaacgctga
    21541 aaaagctgcc atga
//
LOCUS       DAZKGF010000083        21478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZKGF010000083 DAZKGF010000000
VERSION     DAZKGF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.083"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..806
                     /locus_tag="ACHOQV_002860"
     CDS             <1..806
                     /locus_tag="ACHOQV_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9631205.1"
                     /translation="PQLQQQVTDLTSSLNSQEKKELTRKLESIFNNTQVQIAVLIIPT
                     TKDETIEQYATRVFNNWRLGDAKRNDGILIIVAWSDRTVRIQVGYGLEEKVTDALAGD
                     IIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYSANPSESESASSSDHYYFAIFW
                     VFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDLFLFSDKTFSIAVFSFFFTFTIF
                     MVFTCLCVLQKRASGRSYHSDNSGSAGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(866..2164)
                     /gene="eno"
                     /locus_tag="ACHOQV_002861"
     CDS             complement(866..2164)
                     /gene="eno"
                     /locus_tag="ACHOQV_002861"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP9631206.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2252..3889)
                     /gene="pyrG"
                     /locus_tag="ACHOQV_002862"
     CDS             complement(2252..3889)
                     /gene="pyrG"
                     /locus_tag="ACHOQV_002862"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFP9631207.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4117..4908)
                     /gene="mazG"
                     /locus_tag="ACHOQV_002863"
     CDS             complement(4117..4908)
                     /gene="mazG"
                     /locus_tag="ACHOQV_002863"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFP9631208.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     GSAGNSREVLARWEQIKTEERAQKAQHSALDDIPSSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQVVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5004..7238)
                     /gene="relA"
                     /locus_tag="ACHOQV_002864"
     CDS             complement(5004..7238)
                     /gene="relA"
                     /locus_tag="ACHOQV_002864"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFP9631209.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7286..8587)
                     /gene="rlmD"
                     /locus_tag="ACHOQV_002865"
     CDS             complement(7286..8587)
                     /gene="rlmD"
                     /locus_tag="ACHOQV_002865"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9631210.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8644..11400
                     /gene="barA"
                     /locus_tag="ACHOQV_002866"
     CDS             8644..11400
                     /gene="barA"
                     /locus_tag="ACHOQV_002866"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFP9631211.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11624..12964)
                     /gene="gudD"
                     /locus_tag="ACHOQV_002867"
     CDS             complement(11624..12964)
                     /gene="gudD"
                     /locus_tag="ACHOQV_002867"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFP9631212.1"
                     /translation="MSSQFSTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDKCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PQKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12985..14325)
                     /locus_tag="ACHOQV_002868"
     CDS             complement(12985..14325)
                     /locus_tag="ACHOQV_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFP9631213.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVKAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVGLDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14327..15679)
                     /gene="gudP"
                     /locus_tag="ACHOQV_002869"
     CDS             complement(14327..15679)
                     /gene="gudP"
                     /locus_tag="ACHOQV_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFP9631214.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16114..16563)
                     /locus_tag="ACHOQV_002870"
     CDS             complement(16114..16563)
                     /locus_tag="ACHOQV_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP9631215.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     SGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(16581..17363)
                     /gene="truC"
                     /locus_tag="ACHOQV_002871"
     CDS             complement(16581..17363)
                     /gene="truC"
                     /locus_tag="ACHOQV_002871"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFP9631216.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDLIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMLVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17363..17692)
                     /locus_tag="ACHOQV_002872"
     CDS             complement(17363..17692)
                     /locus_tag="ACHOQV_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFP9631217.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDKPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18314..18859)
                     /gene="syd"
                     /locus_tag="ACHOQV_002873"
     CDS             complement(18314..18859)
                     /gene="syd"
                     /locus_tag="ACHOQV_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFP9631218.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18927..19775
                     /gene="queF"
                     /locus_tag="ACHOQV_002874"
     CDS             18927..19775
                     /gene="queF"
                     /locus_tag="ACHOQV_002874"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFP9631219.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19887..21251
                     /gene="ppnN"
                     /locus_tag="ACHOQV_002875"
     CDS             19887..21251
                     /gene="ppnN"
                     /locus_tag="ACHOQV_002875"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFP9631220.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5280 a         5725 c         5128 g         5345 t
ORIGIN      
        1 tcccacaatt acagcaacaa gttacggatc ttacgagtag cttaaactca caagaaaaga
       61 aagaactgac gcggaagtta gaatctattt tcaataatac ccaagtacaa attgctgtat
      121 taattattcc cacaaccaaa gacgagacaa ttgaacaata tgctacaagg gtttttaaca
      181 attggcgttt aggagatgcc aaacgcaatg atgggatact gatcattgtt gcctggtcgg
      241 atcgcactgt ccgcatccag gtaggttatg ggctggaaga aaaggtaacc gatgctctgg
      301 ctggagatat catccgtagc aacatgatac ccgcatttaa acaacaaaaa ttagctaagg
      361 gattagagtt agctataatc gctttgaata accaactcac ttcacaacac caatattcgg
      421 ctaatccttc agaaagtgaa tcagcgtctt ccagcgatca ttactacttt gctatttttt
      481 gggtatttgc agtgatgttc ttccctttct ggttttttca tcaaggcagt aatttttgtc
      541 gcgcatgtaa aagcagtgtc tgtatttcag caatctatct tttagattta ttcctgttct
      601 ccgataaaac tttttccatt gctgtatttt ccttcttttt tactttcacc atatttatgg
      661 tctttacctg tttatgtgta cttcagaaaa gagcatcagg tagaagttat cattcagaca
      721 acagcggttc cgctggaggc tcaggcggcg ggtttagcgg cggtggcggt tcttctggcg
      781 gtggcggcgc atccggccgc tggtaactca accataaaaa acgccagccc gaaggctggc
      841 atttttaaat cagataaagt caggcttatg cctggccttt gatctcttta cgaccgttgt
      901 acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat ttagcaacac
      961 ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca acagccaggt
     1021 cagcgatagt agcgtcttca gtttcgccag aacggtgaga gataactgca gtgtagccag
     1081 cgtctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc tggttgaatt
     1141 tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc ttggtgttgg
     1201 ttacgaacag gtcgtcacca accagctgga ttttgtcgcc cagaactttg gtctggtatg
     1261 cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agatacgatc ggatactgtt
     1321 tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaacgctttg ttgccttcgc
     1381 cagccagaac gtatttaccg tctttgtaga attcagaagc tgcgcagtcc atcgccagag
     1441 tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata acagccagag
     1501 cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaact gcagtgttca
     1561 tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc atgcggatgg
     1621 cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata tcaacgttgt
     1681 tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcatg gagtatttgc
     1741 ccggagtgcc gttcagttca gcgatgtgct cgtacagcgg cataccttta gcagctgcag
     1801 cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat ttggatttgt
     1861 tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct ttagcatctt
     1921 tgccaatcag cgcctgagcg atcgggccgt ttaccgcagc aacagctttg gttacgcctt
     1981 tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg gaaccagtag
     2041 aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt acttcggctt
     2101 caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt acgattttgg
     2161 acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca acgcgtaccc
     2221 agggtacgcg ttgccgttct aactttttta cttacttcgc ctgacgtttc tggaactcgc
     2281 tggcggcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta gaagtaaact
     2341 ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg atctcgacca
     2401 actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc tgtttcaaca
     2461 gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc gcattgtaca
     2521 gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga cgcatggtac
     2581 cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg cgccactcgg
     2641 taatcagcgc cacaactggg tacttacagt ctggcacaaa ttccgtagag ttggcgttct
     2701 ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc aggcaaatac
     2761 ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg ccttctacgc
     2821 cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg atttcaacgc
     2881 cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca cgattcttca
     2941 gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt tcaatgtact
     3001 tgccgaccat accgatggtg acttcactta ccggattcgc ttcttcgaag ataacctgtt
     3061 cccattcgga cagatttgct tccgggcagt ttaagctgaa tcgtttacaa atataatcgt
     3121 ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg tctttcagag
     3181 aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt tcgttcgccg
     3241 gaacggcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg gagagcagct
     3301 cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc atgtacggca
     3361 ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc atctggcgaa
     3421 tcgcttcgag gaacggcagg gattcgatat cacctacagt accgccgatt tcgaccagta
     3481 ctacgtcatg accttcgccg ccttccagca cgcgctcttt gattgcgtta gtgatgtgcg
     3541 gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc agaacgtcag
     3601 agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga atgaaacgct
     3661 cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac acttccccgt
     3721 gttggattgg gctcatagta cctggatcga cgttgatgta cggatccagt ttcatgatgg
     3781 tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca atgcctttac
     3841 ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg ctgaacctga
     3901 gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata cccgaacatg
     3961 acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg aaatcaggag
     4021 tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct tgtcatttaa
     4081 aaaatgacac aaatcgcgct tgaccacgta attcccttag agatcaattt cctgccgttt
     4141 tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt ccagtccacg
     4201 cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg aatttttcgt tcgctttttg
     4261 caatgcgatt tccgcttttg tccctaaatg acgagccaga ttgactgtgg caaacagcag
     4321 gtcacccatt tcctcctcca gtttagcctg gtcgacaaca acctgccgcg cttcgtacat
     4381 cacctcatcg atctcttcgt agactttatc gactaccggg ccaagcgtcg tccagtcgaa
     4441 gccaacgttg gcgcaacgtt tctggatttt ttgtgcgcgc attaaggcgg gtaaactact
     4501 agggatatcg tccagcgccg aatgctgcgc tttttgcgcg cgctcttcag ttttgatttg
     4561 ctcccaacga gcaagaactt cgcggctgtt tccggcagaa ccgtcagcaa aaacatgtgg
     4621 atgacggcgt tctaatttat cgctaatcgc agcgcaaata tcattaaaat caaagcgccc
     4681 ttcttcctgg gccatttgtg cgtaaaacac cacctggaac agcagatcgc ccagttcacc
     4741 gcgcagatcg tcaaaatctt cacgggcgat ggcgtccagc acctcgtagg tttcttcaag
     4801 ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc cgttttccgg
     4861 gtcacgcagg cgctgcatga tggtgagcaa acggtcgatt tgattcatta gaatgtcctg
     4921 aaaattgcgg gtccgtcagg tgtaatacct tcgagtaaaa gatatcgctg gatacggctg
     4981 acgccatatc cagctatgta agactaactc ccgtgcaacc gacgcgcgtc gataacatcc
     5041 ggcacctggt tgagtttacc cagcacgcgc cccagcactt gcaggttgta aatctcaatg
     5101 gtcatgtcga tggtcgccag ttgctgtttg gtgtcgctac ggctggcaac gccaagtacg
     5161 ttcaccttct cgttggcgag aatggtcgtg atatcacgta acaacccact acgatcatta
     5221 gccaccacgc ggaccaccag cgaatatccg gcggagtagc tctcgcccca taccgcgtca
     5281 acaatgcgtt ctggcgcatg ggagcgcagt tccgccaatt gttcgcaatc ggcgcggtgt
     5341 actgaaatac cgcgcccctg ggtaatgaag ccgacaatct catctccagg aatcggctgg
     5401 cagcagcgcg cgatgtggtg catcaggtta ccgacccctt ctaccaccac acgaccgtta
     5461 tctttactgc ggttttgcgg cgtgtagctt ttttgctgaa gctgtttcag cgcggcggcg
     5521 tcctgctctt cggcactcgg cttattaaat tgcgattgca ggaagttcac catctgattg
     5581 agacggatat ccccgccacc aatcgccgcc agcaactcgt cgacatcatt gaagttgtaa
     5641 cgcggcagca gatgtttttc tgcttctttc aggctgatcc ccagatgttc cagctcgtcg
     5701 tcaagaattt gccgcccagc cagaatgttt ttgtcacggt cctgtttacg gaaccaggcg
     5761 tgaattttcg aacgcccacg gctggttgtg acgtaaccga ggtttgggtt taaccagtca
     5821 cggctggggt tcggctgttt ctgggtgata atttcaatct ggtcgcccat ctgcagctgg
     5881 taggtgaacg gcacaatgcg cccgccaatt tttgccccga tgcagcggtg tccgacatca
     5941 ctgtggatgt ggtaagcgaa gtccagcggc gttgatcccg caggcaaatc aacgacatca
     6001 cctttcggcg taaagacgta cactcggtcg tcaaagacct gactacgtac ttcgtcgagc
     6061 atttcgccgg aatcagccat ctcttcctgc cacgcaatca gtttacgcag ccaggcaatc
     6121 cggtcttcat gtcccgaacg tgcgccgcca gcagccgcgc cctctttata tttccagtgc
     6181 gcagcaacac ccaactctgc atcttcatgc atctgtttgg tgcggatttg gatctcaacg
     6241 gttttgccac ccggccccag aaccacggta tgaatggact gataaccgtt tggtttcggg
     6301 ttagcgacgt aatcgtcaaa ctcatccggc aggtggcgat agtgagtgtg cactatcccc
     6361 agtgcggcat aacaatcctg taaacgctcg gcgacaatac gtaccgcacg cacatcaaac
     6421 agctcatcga aggcgaggtt ctttttctgc attttgcgcc agatactgta gatgtgtttc
     6481 ggacggccat acacttcagc tttaacgcct tcagctttca tctcagcgcg cagatggccg
     6541 acaaactctt cgatatagtg ttcgcggtcg agacggcgtt catgcagcag ttttgcgatg
     6601 cgtttgtatt cggtcgggtg gagataacgg aagcagtaat cttccagctc ccatttcagt
     6661 tgcccaatcc caagacggtt cgccaacggc gcgtagatat tggtgcactc ttttgccgcc
     6721 agtacgcgtt catcttccgg cgcatctttt acttcgcgca ggtgagcaat acgctccgcc
     6781 agtttgatga ccacgcagcg aaaatcatcg accatcgcca ataacatccg gcgaacgtta
     6841 tcgacctgtt cggaggaaac agaatcagtg tgcgtcgctt tcagctggcg gatcgccgcc
     6901 atatcacgca cgccgtgaat aaggttaacg accgacttac cgacgctctc acgcagcaca
     6961 tcttcgctga ctacgttggc atcagccaga gggaacagca gcgccgcccg cagcgtgtca
     7021 atgtccatac ttaatgtcga gaggatctcc accatctcaa caccacgcca caataacaga
     7081 ctggcatccg gatgcccctg cgtctgttgc agacaatacg cccaggtttc ggctaagcac
     7141 tcacacgact tctggctggt aatacccaga cttgcgatcc atttttccgg atcaaattca
     7201 ccagccttat tgatatgtgc acttcttacc gcaaccatcg tcctctcctt tagggaccag
     7261 acctgccgaa atcggcaaat cgtaactatt taacgcgcga gaaaagtacc atcgattcca
     7321 gatgtcccgt gtgtgggaac atatccagca tcgccagtcg cgcaatggta tatcctgctt
     7381 ttaataacgc ttcgctatcc cgagccagcg ttgcagggtt acaggataca taaactatac
     7441 gaataggttc cagttttata atttgctgca taacacctgc ggcacctgct cgcgccgggt
     7501 ccaacaacac tttatcgaag ccgtttttcg cccacggctg ctttgtgaca tcttcttcaa
     7561 gattttcgtg ataaaacgtc acattctgta agccattaag acgcgcattc tgctggcctt
     7621 tttccaccag cgccggaaca ccttctacac ccaccacact agcagcttgt gtcgccaatg
     7681 gcagtgtaaa gttgcccata ccgcagaaca gatccagtac gcgatcttca ggttccacat
     7741 ccagccattc caacgcacgc gctaccattt tctggttcac acccgcattg acctgaataa
     7801 aatcgcgcgg gctaaaagtt aagcgcaacc catttgagtc ataccagggc atctcaccag
     7861 agacggtttc gagtatctca ctatcggggg cgagatacag atccaggcct tcagaatgcg
     7921 aaaagcgttc cagtttttcg cgatcagccg aacttagcgg tgcggtatgg cgcaaaatca
     7981 tcagcgtgcc gctggttgcc tgtaccagtt caacatgacc aagatggcgt atagcttgta
     8041 agctgcccag gcatgccctg actttgggca gcaatgcttc aagttggggc actaaaatgg
     8101 gacattgttt aacgtcgaca atgtcactgg agcccgcttt gcgaaacccc atctgaagtt
     8161 gctgtgtttt cggtaagtag ttcagactta aacgcgcgcg acggcgatag ccccagggaa
     8221 catcggcgat cacttcagag acttcgtgtt tcattaatcg ggcgagtgcc gcacttttgc
     8281 ttcgctgctg taaatccacg ctggcgtgtt gttgctgaca gccgccgcat acgccaaaat
     8341 gaggacagcg tggcgtttcg cgttccgggc tatcgcttaa ccggcgcacg actttagcgc
     8401 gggcgtattg ttttttatct tcagtaacag taacttccgc gttttcctgt ggcaataatc
     8461 caggaataaa taacgctttg ccgttatgtc gtgccacgcc ctgaccaaaa gagtcgaggt
     8521 cgttgactga aacagttatg atctgacgcg tcgtcgtgcg tcgttttgca gagtagaatt
     8581 gcgccattgg cgagactttc tcaatttaac agtgtgacct taattgtccc ataacggaac
     8641 tccatgacca actacagcct gcgcgcacgc atgatgattc tgatcctggc accgaccgtc
     8701 cttattggtt tattgctgag tatctttttc gttgtgcatc gctataacga cttgcagcgt
     8761 caactggaag atgccggagc cagcattatt gagccgcttg cagtttctac tgaatatggc
     8821 atgagcctgc aaaatcgcga atctatcggt cagttaataa gcgtactgca tcgtcgccat
     8881 tccgatattg ttcgcgcgat ttcggtttat gatgaaaata accgactctt tgtcacctcc
     8941 aattttcatc ttgatccctc atcaatgcag ctcggcagca acgtgccgtt tcctcgccag
     9001 ctcactgtca ctcgtgacgg cgatattatg atcctccgca cgccgattat ttctgaaagt
     9061 tactcccccg acgaatcgcc cagtagcgat gccaaaaata gtcaaaatat gctgggatat
     9121 attgcgctgg agctggatct taaatcggtt cgcttgcagc aatataaaga gatctttatt
     9181 tccagcgtga tgatgctgtt ttgtatcggt attgcgctta tttttggctg gcgcttaatg
     9241 cgcgatgtaa ccggtccgat tcgcaacatg gtgaataccg tcgaccgcat ccgtcgcggg
     9301 caactcgaca gccgggtgga aggatttatg ctcggcgagc tggatatgct gaaaaacggt
     9361 atcaactcga tggcaatgtc gctggcggct tatcacgaag agatgcaaca caatatcgac
     9421 caggcgacgt ccgatctgcg tgagacgctg gagcaaatgg agattcagaa cgttgagttg
     9481 gatctggcga aaaagcgcgc ccaggaagcg gcgcgtatta aatccgagtt tctggcaaat
     9541 atgtcacacg agctgcgtac accactgaat ggtgttattg gctttacccg cctgacgctg
     9601 aaaacagaat taacaccaac gcagcgcgat cacctgaata cgattgaacg ttcggcaaat
     9661 aatttgctgg caattattaa tgatgttctc gacttctcga aactggaagc cggtaagctg
     9721 attctggaaa gtattccatt cccactacgc agcacgctgg atgaagtcgt taccctgctg
     9781 gcacattctt ctcacgataa agggctggaa ctgacgctca atattaaaag cgacgtgcct
     9841 gataacgtga tcggcgaccc actacgatta cagcaaatca tcactaacct ggtagggaat
     9901 gcaattaaat tcaccgagaa tggcaacatc gatattctgg tagaaaaacg tgcgctgagt
     9961 aataccaaag tgcagattga agtgcagatt cgggataccg gcattggtat tcctgaacgc
    10021 gatcaatcgc gcttattcca ggccttcaga caggctgatg ccagtatttc ccgccgtcat
    10081 ggtggcaccg ggctggggct ggtgattaca caaaaactgg ttaatgaaat gggcggcgat
    10141 atttcgttcc atagcctgcc gaatcgtggt tcaactttct ggttccacat taatctcgat
    10201 ctgaacccga acattattat cgaagggcca tccacccagt gcctcgctgg taagcgcctg
    10261 gcctatgtcg aaccaaactc cgcagcagca caatgcacgc tggatatttt aagtgaaacg
    10321 ccgctggaag tggtttatag cccaacgttc tccgcgctgc ctcccgcgca ttacgacatg
    10381 atgttattag gcatagcggt gaccttccgc gagccgctaa caatgcaaca tgagcgatta
    10441 gcgaaagcag tatcgatgac cgatttcctg atgctggcac ttccttgcca tgcacaagtc
    10501 aatgctgaaa aacttaagca agatggtatc ggcgcgtgtc tgctgaaacc attaacacct
    10561 acgcgcctgt tgcctgccct gacggaattt tgtcatcaca aacaaaacac gcttttgcct
    10621 gtaaccgatg aaagtaagct ggcaatgaca gtcatggcgg ttgatgacaa ccccgctaac
    10681 ctgaaactta tcggcgcatt gctggaagat atggtgcaac atgtggaact ttgcgatagc
    10741 gggcatcagg cggttgaacg ggcgaaacag atgccgttcg atttaatctt aatggatatt
    10801 caaatgccgg acatggacgg cattcgggca tgtgagctca tccaccagct cccgcatcag
    10861 cagcaaacgc cggttatcgc ggtaacggcg catgcgatgg ccgggcaaaa agagaaactg
    10921 cttggcgcgg ggatgagtga ttatctggcc aaaccgatcg aagaagagcg attgcataat
    10981 ttgctgttgc gctacaagcc tggcagcggt atttcctctc gcgtcgtgac gcccgaagtc
    11041 aacgaaattg tggtcaaccc gaatgcgacc ctcgactggc aactggcact acgccaggca
    11101 gcaggaaaaa ccgatttagc gcgcgatatg ctgcaaatgt tactcgattt cctgcctgaa
    11161 gttcgcaaca aagttgagga acagctggtt ggagaaaacc cggaaggcct ggttgatttg
    11221 attcataaac tgcatggcag ttgcggctat agcggtgtac cgcgtatgaa gaatctctgc
    11281 caactgatcg aacaacagct acgtagcggt actaaagaag aagatttaga gccggagctg
    11341 ctggaactgt tggacgagat ggataatgta gcgcgcgaag ccagcaaaat tctcgggtaa
    11401 tggacgtctt gtcgagtagc aaccggattt ttatgacgct ggtttcagat tggtgaaaag
    11461 tgcctgtgtt tatgccggat gcggcgttaa cgccttatcc ggcctacaaa accaattaaa
    11521 ttcaatgaat taaaaaatga tgtaaccctg atacgtagcg caacaggcag tttggcatca
    11581 gtctcaaacc ggctccagat agagccggtt ttggttttct gtcttaacgc accatgcacg
    11641 ggcgcttgtt atcaaacgtc cagccgggga tcaggtactg cattcccatc gcatcgtctc
    11701 gcgcaccaag cccatgtttc tgatacagct cgtgggcttt catcacctga tccatatcga
    11761 tttctacgcc caatcctggt ttttgcggca cctgtaccag cccgcctttg atctcaaacg
    11821 gttctttggt cagacgctga ttgccttcct gccagatcca gtgcgtatca atagcagtaa
    11881 ttttacccgg tgcagcagcg gctacatgag taaacatcgc cagggaaata tcgaagtggt
    11941 tgttagagtg tgaaccccag gtcaggccaa attcatggca catttgcgcc acacgtaccg
    12001 aaccttgcat cgtccagaaa tgcggatccg ccagcgggat atcaacggat tgtaacgaga
    12061 gcgtatggcc catctgccgc cagtcggtgg cgatcatatt ggttgcggtc ggcaggccag
    12121 tcgcgcgacg gaactctgcc atcacttcac gcccggagaa accttgctcc gcaccacacg
    12181 gatcttctgc ataagccagc gaacctttca ggtatttacc aattttaatc gcttcgttca
    12241 gcgaccaggc accgttagga tcgagggtaa tacgcgcctg tgggaagcgt tgcgccagtg
    12301 ccacaataga ttctgcttct tcttccccgg ccagtacacc gcccttcagt ttgaaatcgt
    12361 tgaagccata tttttcatat gccgcttccg ccaggcgcac caccgcatcc ggcgtcatcg
    12421 cttcttcgtg acgcaggcga taccagtcac atttatcatc cggctggctt tgatacggca
    12481 gcggtgtggc tttgcgatta ccgacgaaga acagataacc gagcatttcg acttcgctac
    12541 gctgttgacc atcgcccagc agcgatgcca cgttaacccc cagatgctgc cccagcagat
    12601 ccagcattgc cgcttctatc ccggtaacca catgaatagt ggtccgcagg tcaaatgtct
    12661 gcaaaccgcg cccgccggca tcacgatcgg caaatgtatt acgcaccagc gtcagaacgt
    12721 ttttgtattc acccagcgtt ttacccacca ccagcggaat cgcatcttcc agcgttttac
    12781 ggattttctc gccgccggga atttccccta ccccggtgtg accagaatta tctttgataa
    12841 tcacaatatt acgcgtgaag aacggtgcgt gtgcaccact cagattcatc agcatactgt
    12901 catggcccgc caccgggatg acctgcattt cagtaacaac aggcgtcgaa aattgagaac
    12961 tcatctttat gtcctttttc aaaatcaatg acggccgaaa acgggacgtt tacggtcaaa
    13021 ggtccagccg gggatcaggt actgcatcgg gcccgcgtcg ttacgcgccc ccccaggcag
    13081 acgtttatag gcctcatgcg ccttttgtac ctgctcccag tccagtccca cgcccagacc
    13141 gggcgcatca ggaacggcaa tttttccgtt tttaatctcc agcggatttt tggtcaggcg
    13201 acaatcgccc tcctgccaaa tccagtgggt atcgatagcg gtaggattac ccggtgccgc
    13261 cgcacccaca tgggtaaaca tcgccagaga gatatcaaag tggttattgg aatggcagcc
    13321 ccaggtcagc ccccagtcgt cgcaaagttg cgccacgcgg actgcaccgg aaagcgtcca
    13381 gaagtgcgga tcggcaagtg gaatatctac cgcattgagc atcaccgcat gacccatttc
    13441 gcgccagttg gtggcgatca tgttggtcgc gacgggcaag ccggtcgccc gtcgaaactc
    13501 cgccatcact tcacgaccgg agaaaccctg ctcagcgccg cacgggtctt ccgcataggt
    13561 aaggacatca ttcagccctt tgcacagaga aatggcttca tcaagcagcc aggcaccatt
    13621 gggatcaacg gtaatccgcg catcagggaa gcgtttcttc aatgcacgaa cagtgtcgat
    13681 ttcttgctcg ccaggtaaca cgccaccctt aagtttgaaa tctttaaagc catagcgatc
    13741 ctgtgaggct tccgccagac gcacaacggc ttcgctgttc atcgctttct gatgccgcaa
    13801 ctgataccac tcatggttgc ccggcgtatt ttccagataa ggaagatcgg ttttggtccg
    13861 atcaccgata taaaacagat aaccgagaac ggtaatagca tcacgttgct tgcctggccc
    13921 taacagttcg cagaccggaa cattcagcgc ctgacctaac aggtcaagca aggcggcttc
    13981 cagcgccgcc acggcattaa cgcgcaattc aaaagtccag gcacctttgc cgaaggtatc
    14041 aaaatcggct gcctgattac ctttatgcac ctgctgaacc actttattca ggcgcgcaac
    14101 ttcctggccc agcaccatcg gaatagcctt gaccagtgtt tgataaatca cctctccgcc
    14161 cggcgcttca ccaatgccgg tatgcccggc attatcggtg agtaccacaa tattgcgggt
    14221 gaaatatgcg ttatgtgcac caccaatatt aagcaacatg ctgtcatgtc cggcgaccgg
    14281 aatgactttc atatcagtaa taacaggact ggattgtgtc gtcatcttat tgccccgcga
    14341 caggtttcag ctcaatacgc ttgatatcgc ccaccagcac caggtagctg agcaccgcga
    14401 ttaaggcatg aacaccaaca taaatcagcg ccccattaaa ggagccagtc gtgccaacga
    14461 tataaccaat tgcgattggc gtgacgatgc cagaaatgtt accaaacatg ttaaacaggc
    14521 caccggaaag accgctgatc tcttttggcg cagtatcagc cattacagcc caacccagcg
    14581 caccgatccc cttaccgaag aaggccagcg ccataaagcc gatgatcatc cactcaacgt
    14641 tgacgtagtt gcagaacacc atcaccatcg acaacaacat gcccatcacg atcggtgttt
    14701 tacgcgcaat gttcagtgaa cccgtgcgac gcatcagcca gtcggaaata atcccaccca
    14761 gcacaccgcc gataaaaccg caaaccgccg gaacggaagc cacaaagccc gctttcagaa
    14821 tcgacatccc gcgcgcctgc accagataaa ccgggaacca ggtaatgaaa aagtaagtca
    14881 gggcgttgat acagtactga ccgatataaa cgccgatcat catccgtgac ccgagcagct
    14941 gtttgatctg cccccacttc acgctgaacg gcactttaac tttggtgttt tgctgatcca
    15001 tattgatcag cgcgccaccc gcggcgatgt actccagctc tttctgattt acgcccggat
    15061 gttgatttgg ctcgtggatg actttcaacc agataaagct gatgacaatg cccagaccgc
    15121 ccataaagaa gaagacgtgt gaccagccca cttcatgagt tagccagccc ataatcggcg
    15181 cgaagataac cgttgcgaag tattgggcgg agttaaaaat cgacaccgcc gttccccttt
    15241 cctgcgccgg aaaccaggcc gcaacaatgc ggctgttgcc agggaaagat ggcgcttcag
    15301 caagcccgac caggaagcgc agtgtaaaca gggcaacgat aatgccgaat ccactaaaga
    15361 tatcgacgaa gccttgcagc aaggtaaaca tcgaccagat aaagatcgac cagaagtaga
    15421 cgcgttttga accaaaacga tccagcaacc agccaccagg gatctgcccg ataacatacg
    15481 cccatgagaa ggcagagaac acatagccca ttcccacggg atcaaggccg atatctttgg
    15541 ccatttccga accggcaata gaaagcgtag cgcggtcgcc gtagttgaag gatgtgacga
    15601 taaacaacat caccactatc cagtaacgag cattcgtgcg tttttccaca ctgctcgcag
    15661 cctgacttaa agaactcatt gttgcactcc tgaaaattcg cgttagccac gctcactctg
    15721 gactgcgaca tcgccaggaa atcagaggtg acgtagggtg ttttttgccg tttttatagg
    15781 tcgttcgccg aatacggcgc gtgtttatat ctggcaatag cagtataaaa agcgcgccat
    15841 agcggctcac cgtgcaacaa cacaacatta atgcgttcaa tgaggcccga ttttggcatt
    15901 agccctggac ggtggaatcc acttcacgga aatgaaaaca agaacaagaa aggaagggtt
    15961 aaaacgaaga aataaagaag agtatgaaat ggatcgcttg actccaggca aacgccagta
    16021 aaaatccgcg ctatgaagca gtttttactg gcatttgcct gaaaagattc gattcagcac
    16081 cgctaaaacg gcatttaccg ctcgctgaac atatcaggac aacatcgtgc cccaatgttc
    16141 gacccacgga tttgattccg tttccggttc cgggttctca ctggcgtcaa tcaacagcat
    16201 ttcgccaacc cgctgagcgc tctgttcctg caacaaggca tcgaactgtt tgccactatt
    16261 gcagaaattc acataactac tgtcgccgag cgcaatcacg ccataacgca gattcggctg
    16321 gaagcccagg gtatctttaa ttccctgaaa gagcggcaca atgctatcag gaaggtcgcc
    16381 ctgcccggtc gtggacgtaa ccaccagaac atacttatcc tgatagggca gccagtcgct
    16441 taattcagga tcttcaaata ccgttgcttt gtggccctgc gcggtcagaa ttgcttccgc
    16501 ttcttccgcc actaacagcg aatttccgta catagtgccg acaaaaatac caatttccgc
    16561 cataccgttt tctccctgga ttaggaactt atctctccat cctgacccga tggcgcactg
    16621 aactcaaccc tttcattttc aggaagcaga ccccgccagc caaattgtga taacgcctgc
    16681 atccaggtgt cgtccaggcc cgcgtgaata gtcagtggct cgccagtaaa aggatgcgtc
    16741 aatgacaact gactggcatg cagcattaac cgctgaaggc caaaatgttc agcaccgctg
    16801 cgattctggc gtaaatcacc atgtttgctg tcgccaataa tcgggtggcg caaatgggca
    16861 agatggcgac ggagctggtg cttacgtccg gttttcggct ccagttccac caggccgtag
    16921 cgcgtggtcg ggtaacgtcc ggtcgctacc agcatttcta cggtcgccag accgcgataa
    16981 tgcgtcactg ctggctgcgg gcctttatct tcgcgggcaa atttatcagc gattttgtcc
    17041 agttcttcca ccagtggata atccagcacc gcttcttcca tcaaccagcc gcgcacaatc
    17101 gcatggtaac gtttctggat ttggtgctgt tcaaactgtt gtgccagcag ccgtccggcc
    17161 tcgctggata atcccatcaa caacacacca gaagtgggtc ggtccagacg atgagcagta
    17221 aacacatgct ggcctatcag gtcacgcacg gtttgcatga ccactacttt ctcgtcgcga
    17281 tccagccagc tgcggtgaac cagccagccg gagggtttat ttaccgcaac cagccattca
    17341 tcctgataga gtatttccag cattagctcg catcatccgc aaaaagagca tccagttttt
    17401 ccagctcagc cagaataagc gcgcgttgcg gatgatcagt cgccagcgcc atttcataat
    17461 agggtgcaac ggcaaaagcg cccggtaacg gctggttgtt atctaacaaa tcgtgcattc
    17521 gcgggatcag cacccactgc aaccactcca gtggttccat ggtgtccata aagaacggtt
    17581 gggtactgtt aaattgatgc ggctggggtt tatcgtttcg ccagtgctga tgttcacgca
    17641 gtaacgcttc aagcgcctgc aactggagac gaacgcggtc atgagtggtc ataaagcaac
    17701 ctcaataaga aaaactgccg cgaaggatag caggaataaa aaaagggagc actgtattca
    17761 cagtgctccc ggttcgtttc gcagcattcc ggctactttt gttgctccct gctcatcctt
    17821 gacaactttt cctctggcct tgcggccaat cgttcatcct gaactattgc ttcctgctca
    17881 caccaccccg atgtgatact tcatcctgaa gtgttcctgg ccttcctgac ccaccgaatc
    17941 atcctgaccg gttctcattc tccatcctgg aggtgtcctt taacgcgtcc tgcgtcatcc
    18001 tcttcgcttc atccagaagc ctttccctga aacaccatcc tggtgtgtcc ttcagaagtg
    18061 tcatcatcct gatgttcact tcgttgtctg actccctgtc gacgaagata gaatcgtctt
    18121 tttcgcgaag tcttacaagg cgcttacaga caatgcctta aggaaaattt catatgaaag
    18181 ttaagaatta acaagcaaca tgttgttttt aaatgagaat aacgattctt atctttttaa
    18241 gtattcgctt atttcctggc gatctcctac agagtctgta agagatctct cacaaccaga
    18301 tggggtattt ggattacaga agaggcttaa gttgattaag gaattccgca agatttgagg
    18361 ctaaatgcgt ccgtttacgt gttcctagcg tctctttgca cacttcaccg gagagattac
    18421 acaccgagat gacctctaac tcttcttcca gcgtcgcgat aaatagcgtg ggcggcagtt
    18481 ttagcctttt ctgagtcacc agatgaccaa tcagattttc ctgcacccga cggaaatcgt
    18541 cttcactcca ggtttgcagc aatgtcagtt tgatatcgcc aaactgcgcg tgcatatccc
    18601 cggcaaactg agtcgtataa aaggtatgaa tcgtgggttg tatcacaata tcaaaagcgc
    18661 gttcaaccgc gtttacattt tgttccccgg taaacggctg gggttgccag tatacggcat
    18721 cttcggtagt tgaaataatg cacggtgaag gaacgccgta cagttcctca cttaacggcc
    18781 agcttttatg ctcttcatgc cacgcatcgc agtagcgtgc agtaaaatct ttcagggctt
    18841 gtgcggtcaa atcgtccact aatttctctc ttcacttaaa ccagatacac ttgtcgttta
    18901 gtttatctgg tttatgacgg tgaaacatgt cttcttatgc aaaccatcag gcacttgcgg
    18961 gcctgactct tggaaaatca accgattacc gggataccta tgacgccagc ctactgcaag
    19021 gcgttccacg cagcctgaat cgcgacccgc tgggtctgaa agcggataac ctgccttttc
    19081 acggtacgga tatctggacg ctgtatgaac tttcctggct gaatgcgaaa ggtttgccgc
    19141 aggtcgctgt cggtcatgtt gaacttgatt acaccagcgc aaatctgatt gagtcgaaga
    19201 gttttaagct ctatctcaac agttttaacc agacgcgttt taataactgg gatgaggtgc
    19261 gccagacgct ggagcgcgac ttaagcactt gcgctcaggg taaggttagc gtggcgttat
    19321 atcgtcttga tgaactggaa ggccaaccga taggtcattt caatggcact tgcattgatg
    19381 accaggatat cactatcgat aactatgaat tcactactga ctatctggag aatgccacca
    19441 gtggcgaaaa agtagtagaa gagacgctcg tcagccacct gctgaaatca aattgcctga
    19501 tcacccatca accagattgg ggatcgatcc aaattcagta tcgtggacgc caaattgaca
    19561 gagaaaaact gctgcgttac ctggtttctt tccgtcatca caacgagttc cacgaacagt
    19621 gcgtggaacg catctttaat gacctgttac gcttctgcca gccagagaaa ttgagcgttt
    19681 acgcacgtta tacccgtcgt ggcggtctgg acattaaccc gtggcgcagt aatagcgatt
    19741 ttgtcccatc gaccacaaga ctggttcggc aataaatttt ttctcaattt tgcgtgctgg
    19801 attcacgcag aaggttgtga aaggtcatca ggcagggcta ttgtaatcaa agggaatgac
    19861 gatattcgtc ccataaggag tttttcttga ttacacatat tagcccgctt ggctccatgg
    19921 atatgttgtc gcagctggaa gtggatatgc ttaaacgcac cgccagcagc gacctctatc
    19981 aactgtttcg caactgttca cttgccgtac tgaactccgg tagtttgacc gataacagca
    20041 aagaattgct gtctcgtttt gaaaatttcg atattaacgt cctgcgccgt gaacgcggcg
    20101 taaagctgga actgataaat cccccggaag aggcttttgt cgatgggcga attattcgcg
    20161 ctttgcaggc caacttgttc gcggttctgc gtgacattct cttcgtttac gggcaaatcc
    20221 ataacaccgt tcgttttccc aacctgaatc tcgacaactc cgtccacatc actaacctgg
    20281 tcttttccat cttgcgtaac gctcgcgcac tgcatgtcgg tgaagcgcca aatatggtgg
    20341 tctgctgggg cgggcactca attaacgaaa acgagtattt gtatgcccgt cgcgtcggga
    20401 atcagctggg cctgcgtgag ctgaatatct gcaccggctg tggtccggga gcgatggaag
    20461 cgccgatgaa aggcgctgcg gtcggacacg cacagcagcg ttacaaagac agtcgtttta
    20521 ttggtatgac agagccgtcg attatcgccg ctgaaccgcc taacccgctg gtcaacgaat
    20581 tgatcatcat gccggatatc gaaaaacgtc tggaagcgtt tgtccgtatc gctcacggta
    20641 tcattatctt ccctggcggt gtgggtacgg cagaagagtt gctgtatttg ctgggaattt
    20701 taatgaaccc ggccaacaaa gatcaggttt taccattgat cctcaccggc ccgaaagaga
    20761 gcgccgacta cttccgcgta ctggacgagt ttgtcgtgca tacattgggt gaaaacgcgc
    20821 gccgccatta ccgcatcatc attgatgacg ccgctgaagt cgctcgtcag atgaaaaaat
    20881 cgatgccgct ggtgaaagaa aatcgccgcg acacaggcga tgcctacagc tttaactggt
    20941 caatgcgcat tgcgccagat ttgcaaatgc cgtttgagcc gtctcacgag aatatggcta
    21001 atctgaagct ttacccggat caacccgttg aagtgctggc tgccgacctg cgccgtgcgt
    21061 tctccggtat tgtggcgggt aacgtaaaag aagtcggtat tcgcgccatt gaagagtttg
    21121 gtccttataa aatcaacggc gataaagaga ttatgcgtcg tatggatgac ctgctacagg
    21181 gttttgttgc ccagcatcgt atgaagttgc caggctcagc ctacatccct tgctacgaaa
    21241 tctgcacgta atctccctcc cccggtgacg tttgttgccg ggttttctcg tttttggtca
    21301 catacccatc aatttatttc atttgttata tgaatgtttc ttaccaccct catgcgataa
    21361 atttcattac agttaatatg tcatacaatt tatgttgtaa cgcaaacgtt tccctatttt
    21421 cataaaccac attttatcgc tatgaaaaag aaattatcgc catgattaac taaaagta
//
LOCUS       DAZKGF010000084        21142 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZKGF010000084 DAZKGF010000000
VERSION     DAZKGF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.084"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            53..601
                     /locus_tag="ACHOQV_002876"
     CDS             53..601
                     /locus_tag="ACHOQV_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9631221.1"
                     /translation="MNVNKKKLAEIFNVDIRTIDRWQTQGLPQISGGGKGVEVIFDSA
                     KAIEWYAQREADIENEKLRKEVEDLRAASESDLQPGTIDYERYRLTKAQADAQELKNA
                     REEGLVLETELFTYIFQRVAQNISGILVRVPQTLQRKYPEISPAHLDAMKTEIAKASD
                     VASEAGENVRRWIDDFRRTEGG"
     gene            573..2501
                     /locus_tag="ACHOQV_002877"
     CDS             573..2501
                     /locus_tag="ACHOQV_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9631222.1"
                     /translation="MISDELRAANSAGAIATGLLALKIPVPLTTVQWADRHYYLPKES
                     SYTPGRWETLPFQVAIMNSMGNDRIRTVNLIKSARVGYTKMLLGVEAYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEPTIRDVSVLLDLAPWFGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAAGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2485..2691
                     /locus_tag="ACHOQV_002878"
     CDS             2485..2691
                     /locus_tag="ACHOQV_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP9631223.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2688..4280
                     /locus_tag="ACHOQV_002879"
     CDS             2688..4280
                     /locus_tag="ACHOQV_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9631224.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYHGGGAGFGGQMAEWLPPSQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQQDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRSRFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETRPRET"
     gene            4270..5775
                     /locus_tag="ACHOQV_002880"
     CDS             4270..5775
                     /locus_tag="ACHOQV_002880"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9631225.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALSAEVCQDMQQRIDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEKLAAFPGMTVEAARDILS
                     SSPDKAEPVSASTTAMFERIMANHSPAAVQGGVSQTSADGDADVKMLMAMP"
     gene            5812..6159
                     /locus_tag="ACHOQV_002881"
     CDS             5812..6159
                     /locus_tag="ACHOQV_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9631226.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAAGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFVGTALSHAALP"
     gene            6217..7245
                     /locus_tag="ACHOQV_002882"
     CDS             6217..7245
                     /locus_tag="ACHOQV_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9631227.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPTYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTSAEGIRCYGAIQDAQALSEGVVASSRYPKHWMTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7297..7680
                     /gene="gpFI"
                     /locus_tag="ACHOQV_002883"
     CDS             7297..7680
                     /gene="gpFI"
                     /locus_tag="ACHOQV_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP9631228.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMHSDESGSDQNDTDDGEPLNNTDAPDDVKAVRVRKCLHVMGYCPETGRPVELT
                     FRGMRVLVPSSLAMAMIQHGTAEYA"
     gene            7673..8026
                     /locus_tag="ACHOQV_002884"
     CDS             7673..8026
                     /locus_tag="ACHOQV_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9631229.1"
                     /translation="MRDVQNAFDAALVGVDSTIVEVMGIRVQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            8042..8617
                     /locus_tag="ACHOQV_002885"
     CDS             8042..8617
                     /locus_tag="ACHOQV_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9631230.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMTINRVATTTINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            8614..9009
                     /locus_tag="ACHOQV_002886"
     CDS             8614..9009
                     /locus_tag="ACHOQV_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9631231.1"
                     /translation="MKHTDIRAAVLDALEKHDTGATLFDGRPAVFEEADFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMAAIPALAGMITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            9017..9766
                     /locus_tag="ACHOQV_002887"
     CDS             9017..9766
                     /locus_tag="ACHOQV_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9631232.1"
                     /translation="MATPNPLAPVKGSGTTLWVYTGKGDAYANPLSDDEWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDRGEITP
                     VTGITVTPLTGNVAKGQSTTLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            9783..10205
                     /locus_tag="ACHOQV_002888"
     CDS             9783..10205
                     /locus_tag="ACHOQV_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9631233.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAEQQGTTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
     gene            10232..10645
                     /locus_tag="ACHOQV_002889"
     CDS             10232..10645
                     /locus_tag="ACHOQV_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9631234.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPVCEEEQTEMPDEEDMLMQKAAGVAGGVRFCGVGGGD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            10626..13238
                     /locus_tag="ACHOQV_002890"
     CDS             10626..13238
                     /locus_tag="ACHOQV_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9631235.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISD
                     SFGGPLSLLTLLKEELLGIRDASESSEDSLSDTANALAENARNAGELGRFMSVARVAA
                     GGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAGVISDEAGGGIR
                     QAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALAS
                     LNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIW
                     DDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETT
                     KNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDVRIRQGEMGYINRSRATGV
                     SKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAELRTLEKHSGA
                     NEKISRQRRDLWKAESQYAVLKEAATKRQLSGQEKSLLAHKDETLEYKRQLAELGDKV
                     EYQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEEQRLRDVYGDNPQA
                     LAQVTGALKQTWADEDMLRGDWLAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQN
                     MAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTA
                     IEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEG
                     GYVGGAGSPAQMRRVEGINFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDELR
                     LQLRDGGMLSGSGR"
     gene            13235..13564
                     /locus_tag="ACHOQV_002891"
     CDS             13235..13564
                     /locus_tag="ACHOQV_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9631236.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRTAAGLNADLKTY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            13564..14262
                     /locus_tag="ACHOQV_002892"
     CDS             13564..14262
                     /locus_tag="ACHOQV_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9631237.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMA
                     VNFGGFLSINKLSQ"
     gene            14273..15016
                     /locus_tag="ACHOQV_002893"
     CDS             14273..15016
                     /locus_tag="ACHOQV_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9631238.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVISTPEGERYQPCMNISAEPE
                     AYFRIAPEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHR
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLAEN
                     GFYRVSPSCAQAGDILLCCFGSSVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            15013..15594
                     /locus_tag="ACHOQV_002894"
     CDS             15013..15594
                     /locus_tag="ACHOQV_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9631239.1"
                     /translation="MNTAAEAIRALSLQVPGFRCQMNEGWYQIRIAGEDTAPEAVYAC
                     LHEQLGEGAVIHIVPRLAGAGGNGVFQVVLGVAAIVGSFFTGGATLAAWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
     gene            16307..19837
                     /locus_tag="ACHOQV_002895"
     CDS             16307..19837
                     /locus_tag="ACHOQV_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9631240.1"
                     /translation="MGKGGGKGHTPREAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLERNGHWVTEKDVTINGKTT
                     SQYLTSVILNNLPERPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQLTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLSQQRK
                     VWDVLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPDGILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLD
                     REVILLEAGTSMVNLINGSGKPVRVDITAHPAPDRIQVSALPDGVETYGVWGLSLPSL
                     RRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPMSGSLNSVIPPAVQHLT
                     VEVSASDGQYLALAKWDTPRVVKGVRFSLRLTSGNGENSRLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAAPASIELTPGYYQITAVPHLTVYDPTV
                     QFEFWFSEAKIADTSQVETSARYLGTGRQWSVSGPHIKPGKDFWFYVRSVNLVGKSAF
                     VEASGRASNDAEGYLDFFRGEIGKTHLAQGMWELIDNSQLDDEMAEMKTTITETRNEI
                     TQTVSKTLEDQSATIQQIQRVQTDTNNDLAALYMLKVQKTKNGIPYVAGIGAGIEDAD
                     GQPLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGEPPT
                     FSLTPDGKLTARNADISGNINANSGTLNNVVIAENCTIKGTLRAENIIGDVVKTHNVS
                     LPALSAAGDHRHATERTVTVIDDQSFDRNIVIPPVFYSGIVYQGSDGDGATSICTLTV
                     SVNDRVVFSKSSSIGTGRTSGGYPVQKDTMYPGSSTPFSYFMRMASGGGDVRIKFRIE
                     VIYYGTAPGFMTTGIQITTMKATASGFMES"
     gene            20036..20176
                     /locus_tag="ACHOQV_002896"
     CDS             20036..20176
                     /locus_tag="ACHOQV_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9631241.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHSSLCEFRFRIAGAEIAAFLQC
                     KQ"
     gene            20340..>21142
                     /locus_tag="ACHOQV_002897"
     CDS             20340..>21142
                     /locus_tag="ACHOQV_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9631242.1"
                     /translation="MLKDGTGTAVPGCTIELKARRTTETVIVTTVAYGQPGETGSYSM
                     DVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKALAELEAV
                     AAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERGPKGERGE
                     TGPQGLQGVKGERGEKGEKGEPGGPDATTAQKGIVQLSSATDSDDETKAATPKAVKAA
                     MDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSEFV"
BASE COUNT         4955 a         5044 c         6757 g         4386 t
ORIGIN      
        1 tgtctttttt ggcatttcat gtcgtaactg atttttgata aggaattgat gaatgaatgt
       61 taataaaaag aagcttgctg aaatttttaa tgtcgatata cgcactattg atcgctggca
      121 aactcagggg ctccctcaga tatctggtgg aggaaaaggt gttgaggtca tctttgattc
      181 tgcgaaggcg atcgaatggt atgctcagcg cgaagctgat attgaaaatg aaaaactccg
      241 taaagaggtc gaggatttga gggctgccag cgaatcagac cttcagcccg gcaccattga
      301 ttacgaacgt taccgactga cgaaggcaca ggcagatgca caggagctga aaaatgctcg
      361 tgaggaaggc cttgtcctcg agacggagtt atttacctac atctttcagc gagtggcaca
      421 gaatatatct gggatccttg tccgtgtccc tcagacactg cagcgtaaat accctgagat
      481 atcacccgca catcttgatg ctatgaaaac tgaaatcgcg aaagcatccg atgtggcttc
      541 tgaagccggt gagaatgtgc gcaggtggat tgatgatttc agacgaactg agggcggcta
      601 attctgcagg agcgatagca accggccttc ttgcgctaaa aattcctgtc cccctgacga
      661 cagttcagtg ggcagatcga cactattacc ttccgaaaga gtcatcttac accccggggc
      721 ggtgggaaac actgccattt caggttgcca tcatgaacag catggggaat gaccggatcc
      781 gcacggttaa tctgattaaa tctgcccgtg ttggttatac aaagatgttg ctgggagtgg
      841 aggcttattt tattgagcat aaatcacgca acagccttct tttccagcct acggattctg
      901 ctgctgaaga ttttatgaaa tctcatgtgg aacccacgat cagggatgtg tcggttttac
      961 tcgatcttgc accgtggttt gggcgtaaac atcgtgataa taccctcacg ctgaaacgct
     1021 tttcatcggg tgtgggtttc tggtgcctgg gtggtgcggc agcaaaaaac taccgtgaaa
     1081 aatccgtgga tgtggtctgc tatgacgaac tttcctcgtt cgagccggat gttgaaaaag
     1141 agggctcgcc aactctgctg ggggataagc gtattgaggg ctcggtatgg ccaaaatcca
     1201 ttcgcggctc gacgcctaaa ataaaaggca cctgccagat cgaaaaagcg gccaacgagt
     1261 cggcgcattt catgcgtttt tatgtgccct gcccgcactg tggggaggag cagtatctga
     1321 aatttggcga tgaatccacg ccttttgggc ttaaatggga gaaggacagt cccgaaagtg
     1381 ttttctacct ctgtgaacat catggctgcg tgatccatca gtctgagctt gaccagagca
     1441 acgggcggtg gatctgtgaa aacacgggga tgtggacccg tgacggtctg acgtttttca
     1501 gcgctgcggg taatgaaatt ccgccgccgc gctccatcac attccatatc tggacggcgt
     1561 acagtccgtt caccacctgg gtacagattg tctatgactg gctggatgca ctgaaagatc
     1621 ccaacggcct gaaaactttt gtgaacacca cgctgggcga gacctgggaa gaggccgtgg
     1681 gcgaaaaact cgatcaccag gtgctgatgg ataaggttgt gcgttacacg gcggcggtac
     1741 ctgcccgggt ggtttatctg acggcgggca ttgactcgca gcgtaaccgt tttgagatgt
     1801 atgtctgggg atgggctccg ggagaggaag cctttctggt ggataaaatc atcattatgg
     1861 ggcgtcctga tgaggaagag actctgttac gtgtggatgc ggcgatcaac aaaaaatacc
     1921 gccatgcaga cggaaccgaa atgaccattt cccgtgtctg ctgggacacc ggggggatcg
     1981 atggcgaaat cgtttatcag aggtcaaaaa aacacggtgt tttccgggtg ctgccggtaa
     2041 aaggcgcatc tgtctatggc aagccggtga tcaccatgcc aaaaacccgc aatcagcggg
     2101 gcgtgtatct gtgtgaagtg gggacggaca ccgcaaaaga aattctctat gcccgtatga
     2161 aagccgatcc cacgcctgcg gatgaagcca cgtcgtatgc catccgtttt cctgatgatc
     2221 cggagatttt ttcgcagaca gaggcgcagc aactggtcgc ggaagagctt gtggagaagt
     2281 gggaaaaagg aaagatgcgt ctgctgtggg ataacaaaaa gcggcgtaac gaagcgctgg
     2341 actgcctggt gtatgcctac gcggcattac gtgtgtccgt gcaacgctgg cagcttgatc
     2401 tggctgtact ggcaaaatcc cgggaagaag agacgacccg gccaaccctg aaagaactgg
     2461 cagcgaagct gtccggagga gtgaatggtt acagtcgctg aactgcaggc gctgcgtcag
     2521 gcgcgccttg atttattaac cggtaaacgg gtggtgtctg tccagaaaga tggtcgcaga
     2581 attgaatata cggcggcttc tctggatgag cttaaccgtg cgatcaatga tgcggagtcg
     2641 gtactgggga caacccgacg tcgccgtcgt ccgctgggag tgaggttatg aaacgaacgc
     2701 ctgtcctgat tgatgtgaac ggcgttccgc ttcgggagag cctcagctac cacgggggcg
     2761 gtgcaggatt tggtgggcaa atggcagagt ggttgccacc ctcgcagagt gccgatgcgg
     2821 ccctgctgcc cgcgttgcgt ctggggaatg cccgtgcaga tgatctggta cgcaataacg
     2881 ggatagcggc taatgcggtg gccctgcata aggatcatat tgtcgggcat atgtttctga
     2941 tcagctaccg tccgaactgg cgctggctgg ggatgcggga gactgcggca aaaagctttg
     3001 tcgatgaggt ggaggcggcc tggtcggaat acgccgaagg gatgtttggc gagatcgacg
     3061 tggaaggaaa acgcacgttc acggaattta tccgtgaagg cgtgggcgtt catgcgttta
     3121 acggcgaaat ctttgtgcag ccggtctggg atacggaaac cacgcagtta ttccgcacgc
     3181 gttttaaagc cgtgagtccg aaacgggtgg acacgccagg acacggtatc gggaatcgtt
     3241 ttctgcgggc cggggtggag gtcgatcgat atgggcgtgc cgttgcgtac catatctgtg
     3301 aggatgattt tcctcgctcc gggagtggac gatgggaacg gatcccgcgt gaacttccca
     3361 ccgggcgtcc ggccatgctg catattttcg agccggtgga ggacgggcag acccgtgggg
     3421 ccaatcagtt ttacagcgtc atggaacggc tgaagatgct cgattccctg caggcaacac
     3481 agcttcagtc agccattgtt aaagccatgt atgcagcgac gattgaaagt gaacttgata
     3541 ccgaaaaggc ctttgaatat atcgcggggg caccgcaggg tcagcaggat aatccgctca
     3601 ttaatattct ggagaagttc tccagttggt atgacacgaa tcacgtgacg ctgggcggtg
     3661 tcaaaattcc gcaccttttc cccggtgatg atctgaaact gcagaccgcg caggattcag
     3721 acaatggatt ttctgcgctt gaacaggcgc tgctgcggta tatcgccgcc ggtcttggcg
     3781 tttcctacga acagttgtcc cgtgattact cgaaggtcag ttactcaagt gcccgcgcct
     3841 ccgccaatga gtcgtggcgc tattttatgg ggcggcgaaa atttattgcg gcccggctgg
     3901 ccacgcagat gttttcctgc tggctggaag aggcacttct tcgggggatt atccgtccgc
     3961 cacggtcacg ttttgatttt tatcaggcgc gatcagcctg gtcacgggca gagtggattg
     4021 gtgccggaag aatggccatt gacgggctca aggaggtcca ggaatcagtg atgcgcattg
     4081 aggccggact gagcacgtat gagaaagagc tggcgctgat gggtgaggat tatcaggaca
     4141 ttttccgcca gcaggtcagg gaatccgcag agcgggaaaa agccggactc tcacgtccgg
     4201 tgtggatagc acaggcgtat cagcagcaga tagcggagag ccgcaggccg gaagaggaga
     4261 caagaccacg tgagacgtaa tctttcacac attattgccg cagcattcaa tgaaccgctg
     4321 cttctggagc ccgcctatgc gcgggttttc ttttgcgcgc tcgggcgcga gatgggggca
     4381 gcaagtcttt cggtaccaca acagcaggtg cagcttgatg ctcccggaat gctggctgaa
     4441 acggacgagt acatggccgg aggtaaacga ccggcccgtg tttaccgggt ggtgaacggt
     4501 attgctgtac tgccggtgac cggcacgctg gtgcaccggc tgggtggcat gcggccattt
     4561 tccggaatga caggctatga cggcattgtt gcctgtcttc agcaggcaat ggcggacacc
     4621 tctgtccggg gcgtactgct ggatattgac agtccgggcg ggcaggccgc cggtgcgttt
     4681 gactgcgctg acatgattta ccgcctccgg cagcagaagc cggtctgggc actgtgtaat
     4741 gacacggcct gttctgcggc catgctgctg gcgtcggcct gctcccgacg gctggttacc
     4801 cagacatccc gtatcggctc cattggcgtg atgatgggcc atgtcagcta tgccggtcat
     4861 ctggcgcagg ccggggtgga tatcacgctg atttatgccg gggcgcataa ggtggatggc
     4921 aatcagtttg aagccttatc ggcagaggtg tgtcaggaca tgcagcagcg cattgatgcg
     4981 gcgcaccgga tgtttgccga aaaagtggcg atgtatacgg ggctgtctgt ggaagctgtc
     5041 acggggacag aggctgccgt ttttgaaggt cagtccggta ttgaggccgg gctggcggat
     5101 gagttaatca atgcgtcgga tgccatcagc gtgatggctg cggcgctgaa cacacatgat
     5161 acaggaggca ctatgccgca attaactgca acggaagccg ccgcgcagga gaaccagcga
     5221 gtgatgggga tcctgacgtg tcaggaagcg aaaggacgtg aacagcttgc cacgatgctg
     5281 gcaggacaac agggcatgag cgttgaacag gcccgggcga ttctggctgc ggcagcacca
     5341 cagcagccgg tggcatccgc gcagagtgaa gccgatcgca ttatggcgtg tgaagaagct
     5401 aaaggtcgtg aacaactggc ggcaacgctg gcggcgatgc cggagatgac ggtggaaaaa
     5461 gcccgcccga tcctggcggc ttcaccgcag gcggatgccg gaccctcact ccgtgatcag
     5521 attatggctt tggatgaggc aaaaggggct gaggcgcagg ctgaaaaact ggcggcgttt
     5581 cccggaatga cggtggaggc tgcccgcgac attctgtcct catcgccgga taaagcagaa
     5641 ccggtttctg catccacaac cgccatgttt gaacgcatca tggcgaacca ttcaccggca
     5701 gccgtgcagg gtggcgtgtc acagacgtcg gcagacggtg atgcggacgt gaaaatgctc
     5761 atggccatgc catgaagtca gtgctgacca tcaatatgag gtttttacaa tatggtaacg
     5821 aaaaccatca ctgaacaacg tgcagaagta cgtatttttg ccggtaatga tccggctcat
     5881 accgccacag gcagcagcgg gatttctcag gcaacaccgg cactgacgcc cctgatgctg
     5941 gatgaagcca gcgggaaact ggtggtctgg gacggacaga aagccggtag tgcggctggc
     6001 atactggtac tgccgcttga aggcacagag acggtgctga cgtattacaa gtcgggaacc
     6061 tttgcgacgg aggcaatccg ctggcctgaa agtgtggatg aacacaaaaa ggccaacgcc
     6121 tttgtcggca cagccctgag tcacgcggcg ctgccgtaac acgttatcag gccaccgcgt
     6181 tggcctgact gatttctgaa tgaaaggaac tgatttatgg gattgtttac gacccgccag
     6241 ttactcggtt ataccgaaca aaaagtgaaa tttcgtgcgc tgtttctgga actgtttttc
     6301 cgccgtacgg tgaatttcca caccgaagag gtgatgctgg acaaaattac cggaaaaacg
     6361 ccggtggcag cctatgtctc cccggttgtt gaaggaaaag tgctgcgtca tcgtggtggt
     6421 gaaacccgcg tgttacgtcc gggctacgtc aagccgaaac acgaatttaa ttaccagcag
     6481 gcggtggagc gccttcccgg tgaagatccg gcgcagctga acgacccgac ctaccgtcgt
     6541 ctgcgtatca tcaccgataa cctcaaacag gaagagcacg ccattgtcca ggtggaagaa
     6601 atgcaggcgg tgaatgccgt gctgtatggc aaatacacca tggaagggga gcagtttgat
     6661 actgtcgagg tggatttcgg gcgctctgaa ggaaataaca ttgagcaggc tgacggtaaa
     6721 aaatggtctg agcaggaccg tgatacgttt gatccgacgc atgatattga cctctactgc
     6781 gatcaggcca gcggccttgt gaatatcgcc attatggacg gtacggtctg gcgtctgctg
     6841 aatggcttta agctgttccg cgaaaaactg gatacccgtc gcggctcaaa ttcacaactc
     6901 gaaacggcag tgaaagatct gggcgcagtg gtgtccttca aggggtatta cggcgatctg
     6961 gccattgtgg tggcgaaaac gtcttatgtg gcagaggacg gtaccgaaaa acgttatctg
     7021 ccggagggca cgctggtcct gggaaatacg tcagcggagg gcatccgttg ctatggtgcc
     7081 attcaggatg cgcaggcgtt gtccgaaggt gtggtggcct cttcccgtta tccgaaacac
     7141 tggatgaccg tgggcgatcc ggcccgtgaa ttcaccatga cgcagtccgc tccgctgatg
     7201 gtgctgccgg atccggatga gtttgtggtg gtacaggtga aataatccgg gagcgggggc
     7261 gaaatgcccc cgtgtctttt ttcacagggg gctgagatgg caacaaaaga agaaaatctg
     7321 aatcgtcttc gtcaactggc tggtctgctg gggcgcgagg cggatatgtc ggggagtgct
     7381 gcggatattg ctcagcgtgt gtctgagtgg gaagaggagc ttgctgcttc ccgggagggc
     7441 attatgcact ctgatgagag cgggtctgat caaaatgaca cagacgatgg tgagccgttg
     7501 aacaacacgg atgctccgga tgatgttaaa gccgtccgtg tgcgcaagtg cctgcatgtg
     7561 atggggtatt gcccggagac aggccgtcct gttgagctta cgttccgggg gatgcgtgtt
     7621 ctggtgccat catcactggc aatggccatg atacagcacg gaacggctga gtatgcgtga
     7681 tgttcagaat gcctttgatg ccgcccttgt gggggtggac agcacgattg ttgaagtgat
     7741 ggggatccgt gtgcagttta cctccggagc acaacgtggc ggtgaagttc agggggtttt
     7801 tgacgatccg gagtcgctgg ggtttgccgg tggcggggtc cgtattgaag gaagcagccc
     7861 gtcattattt gtgcggacgg atacggtgcg tgccgtgcgg cgtggtgaca cgctgaccat
     7921 taacggcgag atgttctggg tggatcgtgt ttctccggat gacgggggca gctgttatct
     7981 ctggctcaac cgtgggcaac cacccgctgt taaccggcga cgataaacgc agggtgaaat
     8041 tatggcgata aaagggcttg atcaggcgat tgataatctg agccgggttc gtaaaaacgc
     8101 cattccggcg gcttcagcaa tgacgattaa ccgcgtggcc acaacgacga ttaatcagtc
     8161 ttcatcacag gttgcccggg agacaaaggt acgccggaaa ctggttaagg aacggtccag
     8221 actgaaacgg gccacggtca gaaatccgaa tgccagaatt atcgttaacc gcggtgatct
     8281 ccctgttatt aagctgggga tcagaatgct ggggcgtcgt ccggacagca tactcaaagc
     8341 cggtcagcat cgttatcagc gggcatttat ccagcgatta aataatgggc gctggcatgt
     8401 tatgcaacgt cttccccagg ccagatatga gaagggcaat gacgacaagg gcaggaaaaa
     8461 gcgtaatcgc cttcccattc aggtggtgaa aatcccgatg gcggccccac tgaaacaggc
     8521 gtttgatgaa aacgttgacc gtatccgtcg tgaacgcctg cccaaagaac tggcatatgc
     8581 gctgaaacaa caactgagga ttgcgataaa acgatgaaac atactgatat ccgtgcagcc
     8641 gtactggatg cactggagaa gcatgacacc ggggcgacgc tgtttgatgg tcgccccgct
     8701 gtttttgagg aggcggattt tccggcggtc gcggtttatc tgacggatgc agagtatacc
     8761 ggtgaagagc tggatgctga tacctggcgg gccacactgc atattgaggt gtttttaccg
     8821 gcacaggtac cggattcaga gcttgatcag tggatggaaa gccggattta cccggcgatg
     8881 gcggcgatcc ctgcactggc aggcatgatt accacgatgg ttacgcaggg ctatgagtat
     8941 cgtcgtgatg acgatatggc attgtggagc tctgcagatc tgacttattc cattacatac
     9001 gagatgtgag gatgatatgg caacaccaaa tcccctggcg ccggtaaaag gttccggcac
     9061 cacgctctgg gtttacaccg gtaagggcga tgcttatgca aacccgctgt cagatgatga
     9121 gtggacgcgt ctggcaaaaa taaaggatct gacccccggc gagatgacgg cggaatccta
     9181 cgacgataac tatctggatg atgaggatgc cgactgggta tccaccgggc aggggcagaa
     9241 atccgccggt gacaccagtt ttacgctggc ctggaagccc ggcgagaaag ggcagcgtga
     9301 tttgattgcc tggtttgaca gcagtgagac ccgggcctac aaaatccgct tcccgaacgg
     9361 tacggtggat gtgttccgtg gctgggtgag cgccattggt aaagcggtga ccgccaaaga
     9421 agtgattacc cgtacggtaa aaatcaccaa tatcggtcgt ccttcgctgg cggaggatcg
     9481 gggagaaatc acaccggtca ccggtattac cgtgacgcca ctaacgggga atgtggcaaa
     9541 aggtcagagc accaccctga cagtggccgt tcagccggaa ggcgcaacgg ataaaacatt
     9601 ccgcgcaacg tcggcaaatc agaatttcgc caccattacc gtgaaaggga acacgatcac
     9661 cgtgaaaggt gttgcggcag gtaaagcgca gatccctgtt gtcaccggca acggtgagtt
     9721 tgcggcggtg gcggagatca ccgtcacgga tggcgctgcg ggctgagggg aggagatcaa
     9781 gcatgttcct gaaaacagaa caatttgaat ataacggtgt gtccgtcacg ctgtcggagc
     9841 tgtccgcgct gcagcggttt gattatatga agtttgtttc agacgcagaa caacagggga
     9901 caacgaagca tgatgtcgtg cacattaacc agcgatatct ggaaacggca tccctgcttg
     9961 tggcgatgtc gctatggcat tcccattccc tcaaaggcac tctggcctct ccggagacag
    10021 agatgcagca gatccgccgt gaagtgatgc tgggatggcc tgctgatgca ctgaatcagg
    10081 caacgaaccg ggtgctttat ctttcaggta tgctggataa ccggcacgat gccgatcctg
    10141 aacaaaccgg gaaagcagaa gcgactgagc cggtaacatc aaaaaagcat tcgaaggcga
    10201 gctgaacttt gtcctgaaac tggcgcgaga gatggggaga cccgactggc gcgccatgct
    10261 tgccgggatg acatccaccg aatatgccga ctggcgacgt ttttactgca cgcattattt
    10321 tcaggatacc caactggacg ctcatttttc cgggctgatg tacgccgtac tcagcctgtt
    10381 ttttggcgat ccggatatgc atccggcgga tttcagtctg cttgcgccag tgtgtgagga
    10441 agagcagacg gagatgccgg acgaggaaga catgctgatg cagaaagcgg caggagttgc
    10501 cggaggcgtc cggttctgtg gggtgggtgg gggcgatatt tcaccttctg cggatgtggt
    10561 ggatgtcagc gaagatgatg ttgcattaat gatggcttca gcggggattt ccggaggtgt
    10621 gagatatgtc ccagccagcg ggtgatcttg ttattgattt gagtctggat gcggcccggt
    10681 ttgatgaaca gatggcccgg gtacgccgcc atttttccag tctggaggcg gatgccagaa
    10741 aaaccgccag tactgttgaa caggggctga gccgacaggc gctggctgca caaaaagccg
    10801 ggatatcagt cggacagtat aaggctgcca tgcgcacact gcccgcacag ttcacggata
    10861 ttgtcactca gcttgccggt ggtcagaatc ccttccttat catgctgcag cagggggggc
    10921 agatcagcga ttcattcggt ggaccgctca gcctgcttac cctgctgaag gaggaacttc
    10981 tcgggatcag ggatgcctct gaatcatcag aggattcgct gtcagatacg gcaaatgcac
    11041 tggctgaaaa tgcccggaat gccggtgaac tgggacgatt tatgtcggtg gcccgtgtgg
    11101 cggcaggtgg cggggttgcc gtactggccg cgcttgctgc tgccgcctgg caggcagagc
    11161 aggctgaccg ggccttattg cgttcactga ccctgaccgg aggggcggct gccaccacaa
    11221 cggcagaatt gtggaaaatg gccggggtga tcagcgatga agccggtggt ggtatcagac
    11281 aggcggcaga aaatctggcc cgtctggcag aaagcgggaa atataccgcc gggcagctac
    11341 ggatcatggg ggaaacctct cagagatggc tgcagacggt gggggacgat gccgggaagg
    11401 tggaaaaagc ctttgaaggg attgcagcag atccggtgaa ggcgctggcc tccctgaatc
    11461 agcagtataa cttcctgagc gtttcccagt tacgccatat tgatgagctt gagcgcacga
    11521 aaggtaaaca ggctgcggtg acggaggcga tgtccctgtt tgcggatgtc atgaatgcac
    11581 gtctggagca acttgataaa gcggccacgc cggtggaaaa aatctgggac gatgttaaaa
    11641 cctggacttc tgacgcatgg gcatggatag gtgatcatac actgggggca ctcagtctga
    11701 tcactgacgt ggtggcagga accgttgaac aggtgaagct gctgcttgtg cagggggatc
    11761 tggcgctggc tgaatttatt cagtcagcct gggaaacgac aaagaatgtg cccggcgttg
    11821 gtgcgttgtt tggtgaactg gcagaagaga accgcgtatt tattgagaaa acaaaacgcg
    11881 atgaactggc gctgagaaaa tccattgcgg aacgggatgt gcgtatacgc cagggggaaa
    11941 tggggtacat caaccgttcg cgtgcaacag gcgtcagcaa aggtccgggg cagcaggaag
    12001 ccgtcagccg tctggctgaa gagctgacag gtaaaaagca tacatcaccg aaaacgcgct
    12061 ctgccgggga gagggaagag gagcaggcaa gagaggctct gcttgccctt gaagctgagc
    12121 tcaggacgct ggaaaaacac agcggtgcga atgagaaaat cagccggcaa cgccgtgatt
    12181 tatggaaggc ggaaagtcag tatgcggtcc tgaaagaggc tgccacgaaa cgacagttat
    12241 ccgggcagga aaaatccctg ctggcgcata aagacgagac gctggagtac aaacgccagc
    12301 tggctgagct gggcgacaag gttgaatacc agaaacgcct gaatgagctg gcacagcagg
    12361 cggtgcggtt tgaagagcag cagagcgcga agcaggccgc catcagcgca aaagcccgtg
    12421 gtctcaccga ccgtcaggca cagcgggagt ctgaagagca gcgtcttcgt gacgtgtacg
    12481 gcgataatcc gcaggcgctg gcacaggtca ccggggcact gaaacagaca tgggcggatg
    12541 aagacatgct gcgcggtgac tggctggccg ggctgaagtc cggctggggg gagtgggcgg
    12601 aaagtgcgac ggacagtttt tcgcaggtta aaagtgcggc cacgcagacc tttgatggta
    12661 ttgcacagaa tatggcggcg atgctgaccg gtgcagaggc agactggcgg ggattcaccc
    12721 gttcggtgct gtccatgatg acagaaatcc tgcttaaaca ggccatggtg ggaattgtcg
    12781 ggcgtatcgg cagcgccatt ggtggtgctt tcggtggtgg tgcatctgct tcctcgggga
    12841 cggccattga ggctgcggcg gcgaacttcc atttcgcgac cggaggattt acggggacgg
    12901 gcggcaaata tgagcctgcg gggatagttc accgcgggga gtttgttttc acgaaagagg
    12961 caaccagccg gataggtgtg gggaatcttt accgtctgat gcgcggctat gcggaaggtg
    13021 gttatgtggg tggggccgga agtccggcgc agatgcggcg ggtggaaggt attaatttta
    13081 atcagaacaa tcacgtggtg attcagaacg acggtataaa cggacaggcg gggccgcagc
    13141 tgatgaaggc ggtgtatgac atggcccgca agggggcgca ggatgaactc cggctgcagt
    13201 tgcgtgatgg cggtatgtta tcagggagcg ggcgatgaaa acctttcgct ggaaagtgaa
    13261 gccggatatg gaggtgaact cgcagccgtc ggtgcgtgaa gtgcgttttg gtgacgggta
    13321 ttcgcagcga acggcggcag ggctgaatgc tgacctgaag acgtacaggg tgatgctttc
    13381 cgtgacccgg gaggaggccc ggcatctgga agcgtttctg gcagagcacg ggggctggaa
    13441 ggcatttttg tggaagccac cctatgcata ccggcagata aaggtgacct gtgccgggtg
    13501 gtctgcgcgg gtcgggatgt tgcgtgttga gttcagcgcg gagtttaagc aggtggtgaa
    13561 ctgatgcagg atattcacga agcaagcctg aacgagtcgg ttaagtcaga gcagtcaccg
    13621 cgggtggtgc tctgggaaat cgacctgacg gtgcagggcg gtgagcggta ttttttctgc
    13681 aatgagctga atgaaaaagg ggagccggtg acctggcagg ggcgtgaata tcaggcgtac
    13741 ccgattgagg gcagcggctt tgagatgaac ggaaagggca gcagtgcccg cccgtcgctg
    13801 acggtctcca atcttttcgg ccttgtcacc gggatggcgg aggatttgca gagcctggtg
    13861 ggtgccacgg tggtccgtcg ccgggtgtat gcgcgttttc tggatgcggt gaattttgtg
    13921 gcaggcaatc ctgaggccga cccggagcag gagctgacgg accggtgggt ggtggagcag
    13981 atgtcatcgc tgacggccat gacggcctcg tttgtgctgg cgacaccgac ggagacggac
    14041 ggggcgctgt ttcccggtcg catcatgctg gcgaacacct gtatgtggga ttaccggggc
    14101 gatgagtgcg ggtataacgg tccggcagtg gcggatgagt tcgacaaccc caccacggat
    14161 atccggaagg acagatgcag taaatgcatg cgcgggtgtg agatgcgcgg catggcggtc
    14221 aattttggcg gtttcctttc catcaataaa ctttcgcagt aaatcccgtt ttatgacaca
    14281 gactgaatca gcgattctgg tgcatgcccg gcggtgtgcg ccagcggagt cgtgcggctt
    14341 cgtgataagc accccggagg gcgaacggta ccagccctgc atgaatatct ccgcagagcc
    14401 ggaggcgtat tttcgtattg caccggaaga ctggctgcag gcacagatgc agggggagat
    14461 tgtggcgctg gtccacagtc atcccggtgg tctgccctgg ctgagcgagg cggaccggag
    14521 gctgcagata aagagtgccc tgccctggtg gctggtctgc cggggtgaaa ttcaccggtt
    14581 ccgctgtgtg ccgcacctga ccggacggcg ctttgaacac ggtgtgacgg actgttacac
    14641 cctgttccgg gatgcatacc atctggcggg gataacgctg ccggattttg tgcgtgagga
    14701 tgactggtgg cgcaacggcc agaacctgta cctggacaac ctggcggaaa acggctttta
    14761 ccgggtgtct ccgtcctgtg cacaggcagg cgatatcctg ctgtgctgct ttggttcatc
    14821 ggtgccgaat catgccgcca tttactgtgg caacggtgaa ctgcttcacc atatacctga
    14881 acaactgagt aaacgggaga ggtattctga aaaatggcaa cgacgaacgc attctgtctg
    14941 gcgtcaccgc cactggtccg catctgcctt cacggggatt tacaacgatt tggtcgccgc
    15001 atcagcctgt atgtgaacac ggcagcggag gccatccgtg ccctgtcgct gcaggtgccg
    15061 ggattccgct gtcagatgaa cgaaggctgg tatcagatac gtattgccgg tgaggatacc
    15121 gcgccggagg cggtgtatgc ctgtcttcac gaacaactgg gtgagggagc ggtcatccac
    15181 attgtgccgc gactggccgg agccggggga aatggtgttt ttcaggtggt gctgggggtt
    15241 gcagcgattg tcgggtcatt tttcactggt ggtgcaacac tggcggcatg gggcgcagcc
    15301 ctgagtgccg gagggctgac tgccaccacg atgctgttct cactgggtgc cagcatgata
    15361 ctgggtggtg tggcccagat gctggccccg aaggcaaaga cgccggagta caaaagtacg
    15421 gataacggta aacagaacac gtatttttcg tcactggaca acatgattgc ccagggtaac
    15481 ccgatgccgg tgccttacgg tgaaatgctg gttggttcac gacggatatc ccaggacatc
    15541 agcacccgtg atgagggcgg agacgggaaa gtggtggtta tcgggcgggg ataaaaataa
    15601 aaaaaatccc gcagagttag cggagctgcg ggaaataacg atgaagatta acgttatgga
    15661 gttatttttt tgggcagaaa aacattaacg cagcgaaatt ataagcgcta catggatttt
    15721 gcgaaaatgt gaagaaattc agaaattttt tgctgcagag gtgcagggag cttgccgggt
    15781 atttgtggca gaagacagaa aacatcatgc cggggttaag ggataaaaaa atcccgcaga
    15841 gtccggagac tgcgggacag aaccgatgaa gattaacgtt caaggagttt tgtttttatt
    15901 gacccgaaaa aactgtaacg ccccggaatg atatctgcca cggggacgta cagaaaatgt
    15961 gaagaaattc agactttttc tgtcgcaggt ttacagaacg gatgaccgcc agggtaccag
    16021 tggcgtaaat gagcaggatg ttgtggtgtg gctgaagcaa aaaaatctcc cgtaatatcg
    16081 ccggatactg acgggagacg aatagtcacc ttaaggaggt ctgataatta ttgcttttat
    16141 tgagcaaaaa aactgtaacg ctgcgtcatt atagtggcta caggcaattg ccggaaatgt
    16201 gaagaatttc agaaatttta ttccgtcatg acacaggcac cctccggggt gcctgttgtt
    16261 tttgggcata aacagattca gacatcagac aggagagggg gacagagtgg gtaaaggggg
    16321 cggcaagggg cacacaccgc gtgaggcgaa ggacaatctc aaatccacgc agatgatgag
    16381 cgtgattgat gccattggtg agggaccggt ggaaggtccg gtgaagggac tgcagagtat
    16441 tctggtgaac aaaaccccgc tgacggacac ggacggtaat cccgtgatac acggtgtgac
    16501 cgccgtctgg cgtgccgggg agcaggagca gacaccgccg gaaggctttg agtcctccgg
    16561 ggcagaaacc gcactgggcg tggaggtgac gaaggcaaag ccggtgacgc gcaccatcac
    16621 gtcagcgaac attgaccgtc tgcgggtcac cttcggggtg cagtcactgg tggagaccac
    16681 ctcaaagggt gaccgtaatc cctcttctgt ccgcctgctg attcagcttg agcgtaacgg
    16741 gcactgggtg acggagaagg atgtcaccat taacggcaag accacctcgc agtacctgac
    16801 gtcggtgatt ctgaataatc tccctgagcg cccctttaac atccggatgg tcagggagac
    16861 ggcggacagc accacggacc agctgcagaa cagaacgctg tggtcgtcat acaccgaaat
    16921 catcgatgtg aaacagtgct acccgaacac ggccattgtg gggctgcagg tggatgcgga
    16981 gcagtttggt ggccagcagc tgacggtgaa ctaccatatc cgtggtcgca tcattcaggt
    17041 gccgtcaaac tatgacccgg aaaaacgcac ctacagcggt atctgggacg gcagtctgaa
    17101 accggcatac agcaataacc cggcctggtg cctgtgggac atgctgaccc acccgcgcta
    17161 cggcatggga aaacgcctgg gggccgcgga tgtggacaag tgggcactgt atgccatcgg
    17221 gcagtactgt gaccagacgg tcccggatgg tttcgggggc acagagccgc ggatgacttt
    17281 taatgcgtac ctgtcacagc agcgtaaggt ctgggatgtc ctgggggatt tctgctcggc
    17341 gatgcgctgt atgccggtat ggaacggcca gacgctgacg ttcgttcagg accgcccgtc
    17401 ggatgtggtg tggccctaca ccaacagcga tgtggtggtg gatgataacg gcgtggggtt
    17461 ccgctacagc ttcagtgccc tgaaggaccg gcacacggcg gtggaggtga attacaccga
    17521 cccgcagaac ggctggcaga cttccacgga actggtggaa gacccggacg gcatcctgcg
    17581 ctacgggcgc aacctgctga agatggatgc gttcggctgt accagccgcg gtcaggccca
    17641 ccgtgccgga ctgtgggtga taaagaccga actgctggaa acgcagacgg tggatttcac
    17701 gctcgggtca caggggctgc gtcacacacc cggtgacatc attgaaatct gtgataacga
    17761 ctatgccggg accctgaccg gcggacgcat cctgtccatc gatgccgcca gccgtaccct
    17821 gacgctggac cgtgaggtga tactgctgga agcagggaca tcgatggtga acctgattaa
    17881 cggcagcggt aagccggtgc gcgtggacat cactgcacac cccgcccctg accggataca
    17941 ggtcagcgcc ctgccggatg gcgtggagac atacggtgtg tggggactct ccctgccgtc
    18001 actgcgtcgt cgcctgttcc gctgtgtttc catccgggaa aacacggacg gtacctttgc
    18061 catcacggcg gtgcagcatg tgccggaaaa agaagccatt gtggataacg gggcccgctt
    18121 tgagccgatg tccggctcac tgaacagcgt catcccgccg gcggtgcagc accttacggt
    18181 ggaggtgagt gcctcagacg gccagtatct ggcactggcg aaatgggaca cgccgcgggt
    18241 ggtgaagggc gtgcgcttca gtctgcgcct gaccagcgga aacggtgaaa acagccgcct
    18301 ggtgaccacc gccatcactg ccgacacgga gcaccgtttc agtggcctgc ctctggggga
    18361 atacaccctg acggtcaggg cgataaacag ctacggccag cagggcgaac ctgccaccac
    18421 cacgttccgg attaacgcac cggcagcacc ggccagcatt gaactgacgc cggggtatta
    18481 tcagataacg gcggtcccgc atcttacggt gtatgacccg acggtacaat ttgagttctg
    18541 gttttcggag gcaaaaatcg cagacacatc tcaggtggaa acctctgccc gttatctggg
    18601 gacaggccgc cagtggagtg tctccggccc gcacattaag ccggggaagg atttctggtt
    18661 ttatgtgcgc agcgtcaacc tggtggggaa atctgcgttt gtggaagcca gtggccgggc
    18721 cagcaatgat gcggaagggt atctggactt tttcagagga gaaatcggga agacacatct
    18781 ggcacagggg atgtgggagc tgattgataa cagccagctt gatgatgaga tggcggagat
    18841 gaagaccacc atcacagaaa cccgcaatga aatcacgcag acggtcagta aaaccctgga
    18901 agaccagagt gccaccatac agcagataca gcgggtgcag acagacacaa ataacgacct
    18961 ggctgcgctg tacatgctga aggtgcagaa aacgaaaaac ggcattccgt atgttgccgg
    19021 tataggtgcg gggattgagg atgctgatgg ccagcccctg agcaatatac tgctgcaggc
    19081 ggaccgtatc gcgatgatta acccggagaa cggcaacacc acgccgctgt ttgtggcgca
    19141 ggggaatcag ctgttcatga acgatgtgtt cctgaaacga ctgtttgcgg tgagtattac
    19201 ctcatcaggt gagcctccga cgttttccct gacgccggac gggaaactga cagcccgtaa
    19261 tgcggatatc agcgggaata ttaatgcgaa ctcgggcacg ctcaataatg tcgtgatagc
    19321 ggagaactgt accatcaaag gtactctgag agcggaaaat atcattggcg atgtggtgaa
    19381 aacgcacaat gtcagtcttc ctgctctcag tgcagcaggg gatcacagac acgcgacaga
    19441 acgaacggtt actgtcattg atgatcagtc gtttgaccgg aacattgtta tcccgccggt
    19501 tttttattcc ggtattgtct accagggaag tgatggtgat ggtgcaactt caatctgtac
    19561 gctgacggta agtgtgaatg atcgggtagt gttcagcaaa agctcatcca tcgggaccgg
    19621 gcgtacgtca ggtggatacc ccgttcagaa agacaccatg tatcccgggt catccactcc
    19681 gttcagctat ttcatgagga tggcgtcagg gggaggagat gtcaggatca agttccggat
    19741 tgaggtcatc tattatggaa ctgcaccggg ttttatgacc acagggatac agatcacaac
    19801 gatgaaggcg acagcctcag gatttatgga aagttaatcc cggacgatcg tgattgccgg
    19861 aaggagacaa aaaccgtaca gtatgcgcgg gtgcctttgg ctgatggccg gagggaacac
    19921 ctgaaggccg gaaagacgaa ggccccggac aaacattcat gtttaacccg aggcctgact
    19981 ttcgatcttc agcaaattga aggttagcgc ctctccgtaa aaggagcaag cgtttatgtc
    20041 gcaaaaaccg ttaaaaacca ccgtgatttg tatcacggta gtgctcatta tctggatcac
    20101 ccacagttca ctgtgcgagt tccggttccg gatagcgggc gcggagattg cggcgttctt
    20161 acagtgtaag cagtaagaaa ccgtggcggg ggagaatatc ccccgccgac cggttgctga
    20221 gggtggtcag ccggatggca ccgttttaac accaacaaac cacaaatttt accgcaggcc
    20281 gggaaaccgg tactgcggtt tttttatggg ggaaatccat gacagtcaga atatcgggtg
    20341 tgctgaagga tggtacggga acagcggtac cgggatgcac gatagagctg aaagcgcgcc
    20401 gcacaacgga gacggtgatt gtcaccacgg tggcgtatgg tcagccgggg gaaaccggca
    20461 gttacagtat ggatgttgag ccggggttgt accgggtgac gctgaacacg gaagggtacg
    20521 cgccgtcata tgtgggtgac attctggtga aggcggattc tgcaccggga acgctgaata
    20581 aatttctgat ggacctggag gacgcacagt attacccgaa agcccttgca gagctggaag
    20641 cggtggcagc ggaaatcctg aaacgtgcag aagcgtcagc ggccagtgca gaggaagcga
    20701 agaaacgggc agagaatgcg cggggaccga agggggataa gggagacacc gggccgcagg
    20761 gtattcccgg gccaaaaggc gataccggcg agcgggggcc aaagggtgag cgtggtgaga
    20821 caggaccaca ggggcttcag ggtgtgaaag gtgaacgggg agagaagggc gaaaaaggcg
    20881 agccgggagg accggatgcg acgacggcac agaagggaat tgtgcagtta agcagcgcaa
    20941 cggacagtga tgatgaaacg aaggcagcca ccccgaaagc ggtgaaagcg gcaatggaca
    21001 aagcggacgg atgcctggag aaagcgaaaa acggtgacga tatcccggat aaggtgaagt
    21061 ttctgaacac cgtgggagca gccagagtat acgggcggga cattcatacg ggggccggtg
    21121 aatggaccac gagcgagttt gt
//
LOCUS       DAZKGF010000085        21014 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZKGF010000085 DAZKGF010000000
VERSION     DAZKGF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.085"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(132..662)
                     /gene="cybB"
                     /locus_tag="ACHOQV_002898"
     CDS             complement(132..662)
                     /gene="cybB"
                     /locus_tag="ACHOQV_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFP9631243.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHEMLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            851..1852
                     /gene="gap"
                     /locus_tag="ACHOQV_002899"
     CDS             851..1852
                     /gene="gap"
                     /locus_tag="ACHOQV_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9631244.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILAKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(1893..3332)
                     /gene="aldA"
                     /locus_tag="ACHOQV_002900"
     CDS             complement(1893..3332)
                     /gene="aldA"
                     /locus_tag="ACHOQV_002900"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFP9631245.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISTLIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3529..4329)
                     /gene="ydcF"
                     /locus_tag="ACHOQV_002901"
     CDS             complement(3529..4329)
                     /gene="ydcF"
                     /locus_tag="ACHOQV_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFP9631246.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEMPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(4601..8503)
                     /gene="hrpA"
                     /locus_tag="ACHOQV_002902"
     CDS             complement(4601..8503)
                     /gene="hrpA"
                     /locus_tag="ACHOQV_002902"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFP9631247.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELSLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            8704..9309
                     /gene="azoR"
                     /locus_tag="ACHOQV_002903"
     CDS             8704..9309
                     /gene="azoR"
                     /locus_tag="ACHOQV_002903"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFP9631248.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            complement(9363..10655)
                     /gene="ynbD"
                     /locus_tag="ACHOQV_002904"
                     /pseudo
     CDS             complement(9363..10655)
                     /gene="ynbD"
                     /locus_tag="ACHOQV_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
     gene            complement(10669..12426)
                     /gene="ynbC"
                     /locus_tag="ACHOQV_002905"
     CDS             complement(10669..12426)
                     /gene="ynbC"
                     /locus_tag="ACHOQV_002905"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFP9631249.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLASSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIISDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALVFEKMQ
                     SFISHLYANKSQKLDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDIFLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIDPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRGN"
     gene            complement(12442..13338)
                     /gene="ynbB"
                     /locus_tag="ACHOQV_002906"
     CDS             complement(12442..13338)
                     /gene="ynbB"
                     /locus_tag="ACHOQV_002906"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9631250.1"
                     /translation="MLEKSLATLFALLILATLINRFLVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWVTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGLNC
                     FELFLLFIPLFGFLILATWRVFVGEPSGFLHTVSAIFCGWIMTVFALSHAAWLLMLPT
                     TNIQGEALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     VSLIIGPLLTPLDTLQALLAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(13338..13943)
                     /gene="ynbA"
                     /locus_tag="ACHOQV_002907"
     CDS             complement(13338..13943)
                     /gene="ynbA"
                     /locus_tag="ACHOQV_002907"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9631251.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLHKRQVSANQITLAALALSLLTGL
                     LLALVPYHGMFLLLPIVLFVRMALNALDGMMARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWVL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMNAEK"
     gene            complement(14112..15457)
                     /locus_tag="ACHOQV_002908"
                     /pseudo
     CDS             complement(14112..15457)
                     /locus_tag="ACHOQV_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(15521..16381)
                     /gene="pdxI"
                     /locus_tag="ACHOQV_002909"
     CDS             complement(15521..16381)
                     /gene="pdxI"
                     /locus_tag="ACHOQV_002909"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFP9631252.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLGALDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(16612..17202)
                     /gene="paaY"
                     /locus_tag="ACHOQV_002910"
     CDS             complement(16612..17202)
                     /gene="paaY"
                     /locus_tag="ACHOQV_002910"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFP9631253.1"
                     /translation="MPIYQIDGLTQFVPEESFVHPTAVLIGDVILGKGVYVGPNTSLR
                     GDFGRIVVKDGANIQDNCVMHGFPDQDTVVEEDGHIGHSAILHGCIIHRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRSKQTLHQVEPLREVEPGRQRLVFDENLRPKQ"
     gene            complement(17184..17336)
                     /locus_tag="ACHOQV_002911"
                     /pseudo
     CDS             complement(17184..17336)
                     /locus_tag="ACHOQV_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
     gene            17337..17543
                     /locus_tag="ACHOQV_002912"
                     /pseudo
     CDS             17337..17543
                     /locus_tag="ACHOQV_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(17715..18041)
                     /gene="ydbL"
                     /locus_tag="ACHOQV_002913"
     CDS             complement(17715..18041)
                     /gene="ydbL"
                     /locus_tag="ACHOQV_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFP9631254.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(18049..18234)
                     /gene="ynbE"
                     /locus_tag="ACHOQV_002914"
     CDS             complement(18049..18234)
                     /gene="ynbE"
                     /locus_tag="ACHOQV_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFP9631255.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(18231..20870)
                     /gene="ydbH"
                     /locus_tag="ACHOQV_002915"
     CDS             complement(18231..20870)
                     /gene="ydbH"
                     /locus_tag="ACHOQV_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFP9631256.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVVARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGHLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWSE
                     DEPLLLTDVSVDVLGGNVLMKQLRMPQHDSALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNAHLPGNDCPQGKECEDKQ"
BASE COUNT         5500 a         5558 c         4762 g         5194 t
ORIGIN      
        1 tggacgtatg ggcaaggatt atggttttat ttgtcataca aataaatata ataggcgctt
       61 ccgatgtaga cccgtattct tcgcctgtac cacgggtcgg ttttagtaca ggcgttttct
      121 ttaaatatcc ttcaggaacg tttacgcggc atcatgcgta gaagtgtgtt gtccttccag
      181 aaatagtggt gtgccagtgc agccgcagcg tgcaacccga tgacaaaata tcccagattc
      241 gccagcattt catgccacga ctttaagcta tctacccgtt cgaaattggc ctctgaagcg
      301 taaggcatcg tcaaaccaaa cgcaaaccac gggttgcccc ggttatacat catcaccaaa
      361 ccaatcactg gcagcgcaat aaacaggaga taaatcacca aatggcccaa atgcgccagt
      421 cccgtcatca tcggttttgg cttaggtata atcggcgggg ttgggtattt cagtcttaac
      481 aaaagacgaa ccaccatcag tacgagaatt gagatgccac aggaaacatg aatcatgttg
      541 atgaggggcc gatcgctacg tgggaagaaa ccacgaaact ccattgcgca atacgctacg
      601 ataaccagca aaaagaccag ccagtgaatg ctgatttgta accttgagta tttatttccc
      661 ataacatttc ctgctttaac ataatttgcc gttaacataa cgggcttttc ttaaaatttc
      721 attaaatatt gttcacccgt tttcagtacg ttacgtttca ctgcaagaat gtagattgcc
      781 catcccgcca tcctggtcta agcctggaaa ggatcaattt tcatccgaac gttcctgaca
      841 ggagaagacc atgagtaaag ttggtattaa tggttttggt cgtatcggtc gactggtgtt
      901 gcgtcgatta cttgaagtca aaagcaacat agacgttgtc gctattaatg atctcacttc
      961 cccaaaaatt ctcgcctacc tgctgaaaca tgattcaaac tacggaccgt tcccctggag
     1021 cgttgatttt acggaagatt cacttatcgt tgatggaaaa agcatcgcgg tttacgccga
     1081 aaaagaggct aaaaatattc cgtggaaagc gaaaggggca gaaattattg tcgaatgtac
     1141 tggcttttat acctccgccg agaaatcgca ggcgcatctt gatgctggtg cgaagaaggt
     1201 gttgatttcc gcccctgccg gtgaaatgaa aactatcgtt tataacgtca atgacgacac
     1261 tctggatggc aacgacacca ttgtttccgt ggcgtcatgc accactaact gccttgcgcc
     1321 gatggccaaa gccttgcatg acagtttcgg gatagaagtc ggcacgatga cgaccattca
     1381 tgcctatacc ggcacccagt cactggtgga tggtccgcgt gggaaggatt tacgagcttc
     1441 acgcgcagcg gcagaaaata tcattcccca cactacaggt gcggcaaaag ccattggtct
     1501 ggtgatcccg gaactgagcg gcaaactgaa aggtcatgcg caacgcgtgc cggtgaaaac
     1561 aggttcggtc actgagctgg tgtccattct cgcaaaaaaa gtgactgccg aagaggtgaa
     1621 taacgcactt aaacaagcaa ccaccaataa cgagtcattt ggttataccg atgaagaaat
     1681 agtctcttcc gatatcattg gcagccattt cgggtcggtg tttgatgcca cgcaaacgga
     1741 aattaccgcc gtgggcgatt tacaactggt gaaaacggtc gcctggtacg ataacgaata
     1801 tggcttcgtc acacaactca ttcgcaccct cgaaaaattc gctaaactct gacgcacaca
     1861 ggcggaggaa aaacctccgc ctcttccact cattaagact gtaaataaac cacctgggtc
     1921 tgcagatatt catgcaagcc atgtttgcca tctgcgccgc caataccaga tttacgccat
     1981 ccggcgtgga atccttgcat agcttcgaag ttttcacggt tgatgtaagt ttcaccaaac
     2041 ttcagccctt taatggcttt catcgcgacg ttcagatttt gggtatagat tgatgaggtc
     2101 aggccgtaat cactatcatt cgccattgag atagcctctt ccagcgtgtc aaatgcgacc
     2161 accggcagaa ccgggccaaa ggtttcctca tgcataatcg acatttcctg gcgaacatcc
     2221 agcagcaatg tcggcggata ataatatcct ttcccctcta ccgctttgcc acccaacgcc
     2281 actctcgccc cttcttctac tgcgcgcgcc actttttgct cgaccctttc cagcgccgcg
     2341 gcgttaatca acggccccat cgcaatgtcg ttgcgttcag cggggttacc aaattgaacc
     2401 gcctgcatcg cttcacccag ccgattgaca aactgatcat aaatgccttt ctgtacataa
     2461 acacgttctg cacagttaca cacttgccca ctattaatga cgcgtgaatc aacgatggct
     2521 ttgactgcca gttcaagatc ggcatcgtcc attacgatag ccggtgcttt accccccaat
     2581 tccaggcaca ctttggtgat gtttttcgcc gcagtcgcca taatcttttc acctgcagag
     2641 acgctgcctg tcatactgac cattgcgacc tttgggttac ccgccagttc ttgcccaacg
     2701 gtttcaccac gccccagaac aaggttaaac acgccgcgcg gaaggcctat ttcatcgaca
     2761 attttggcga atgcaatcgc attgtttggc gtaaattcac tgggtttaat gacgatggta
     2821 ttaccggtca aaagagcggg agccattttg cgggcaatga ggaagaacgg gaagttccac
     2881 ggcagaatgc cggtagtcac gccaagcgca cgtttaaaca gaagaatatt ttctcctgga
     2941 cgatcgcttt gaataatctc gccttcataa cgccgtgccc actccgccat gtaatcgata
     3001 tagtccgcag taaaagccac ttcgacttca gccagctgct ggatcttacc cccttcttca
     3061 acaatcagcg tactgatttc actggcgcgt tcgcggatcc cggcggagat tttgcgcaac
     3121 caactggcgc gttcaatagc aggcaacgct tcccattctg gttgtgcacg ttctgctgca
     3181 tcgattgcct tacgggcatc ctcggcctga ccatcgggaa tgcgggaaat gacagcctct
     3241 gtagcagggt ttaccacatc aatccatgcg tctccacgcc aggtaacaaa ctgtccatca
     3301 atatacatag gatgttgaac gggtactgac atgagcgact cctgtgattt atatgtttgt
     3361 ttttggtaaa tgtattgtta attaacatca atcatcatag agacggcatc atgaaaggac
     3421 aattctgtga agggcttcat aaaaacaagt ataaaaggaa taagaggcag aaataaaagg
     3481 caggagttat gcccgtttgc aaacaagtgg ttcacaaacg ggcaattttt aacgtaacga
     3541 gcgactttcc atcgcctcga ttagcaccgc atcgtgtttc agcgtttgcc atgcatggat
     3601 gacttctgcc ggaaaatcaa cgtgaacgat aaaatctcgc ccgcggggac cgtagccatc
     3661 gctatcatcg cgtaaacgcg gcagctcgcc agtgagtagt gagagataac gctcaaccgg
     3721 ccataatcct tgcaacggat taacaaagat tacactgtct gcgttatttc ctaactgagg
     3781 aacgaatccg ggataactta accagcgtgg tgcatcggga ttgtccccag tcatacggcg
     3841 gaacgtcgcc atcgtgcgcc gctgcatggt ggggtcctga acaacgatag ccgtatgaac
     3901 tcgttctacg gcctgattca atagcgcgat gctaaagcgt gcgttttcac cgcagtttgt
     3961 tgactggtct tcaatccaga ttttttcatg cggaatgtgc cagaactgat gagcgatatc
     4021 cgccaggatg gtcgcttctg ctctgccagt ggtgcggata gtgttgtagt gcggatgctg
     4081 tgcgatggcg ctatacaaaa aagttgtcga gtgaccgata ccaccactaa tcagtagagg
     4141 aatttgctga tcgcgagcaa tcttacatgc cgcgtcgata gtcggcataa ccgcattgcc
     4201 tgcaaggatc acgcaatcgg cctgatacgg catctcaccg gagaaatcat cctgcgccag
     4261 ccactgaccg ataacattta tggcatctat ggttgccggc gaaagcgtcg gaaacggggt
     4321 aatgttcatg gttcctcctt atcgttccct taagaatact gcgtgagata atagtgagaa
     4381 gcggagcact ctgaaaggag gaagtcgtat ttccttaacc gccactccgc aagcctgaca
     4441 tatcttttaa ggaaatgctg tagataaaca ttattcatct tgcagctgag aattgtattt
     4501 ttctgaattc ctgaattttt gcccttaccg ttttagaatt cataaaaaac ccggcgctaa
     4561 atgcttaccg ggttttagat ttatcaggca aatagccgcg ttaaccgcta atctgttcca
     4621 tcgcctgcaa aatacgctta tctgaaatcg gataaggcgt accaagttgt tgagcgaagt
     4681 aactaacgcg caactcttct atcatccaac ggatctcttt cacgtcttca tcctcacgac
     4741 gtgcgggcgg cagtttgttg atccattgct gccacgcctg ctggacattt tcgactttca
     4801 gcatctgagc acggtcacga tgcggatcaa ccgccagttt ttccagtcgt ttttcaatcg
     4861 cctgcaaata acgcagcgtg tcgcccagcc gtttaaaacc gttaccagtg acaaaaccgc
     4921 gatacaccag cccgcccatc tgcgctttga tgtcagaaag ccccagcgcc atagtcatat
     4981 ccacccgccc tttcaggcgt ttgttgatat tgaacactgc cgtaaggatt tgctcgacct
     5041 gcttcgcaat atccaccacc gtatcgttca gttcagcgcg gactttctca tgcagcgcag
     5101 caaagccttc ttccgtccag accgggcctc cattggcgtc gatcagttga tccaccccgc
     5161 aggagataca gtcgtcgatc agctccagca ctttgccata cgggttaaag tacagcccca
     5221 gcttggcttt gttcggtaac ttttcatgca aatacttgat cggcgatgga atattcagca
     5281 gcagtaaacg acgaagaccg ttccacatcg cttgcttttg ttccagcggg ttatcaaaca
     5341 gtttgatcgc cacgctgtcg cgctcatcca ccaacgctgg ccacgccttc actttgtagt
     5401 tgccacgctt ctgttcgtag ctttccggca gttgcccaaa gctccagata tgtaagccgc
     5461 tctgctcgat accgtcatcc gccaccgcag acagcgtttc ctgcactttg cctttcagag
     5521 catctttcag atcctgtaat gagcgccctt cttttagctt cttgtttttg tcatccacca
     5581 cacggaaggt gattttcagg tgatcgggca cctgatccca gtgccagtct tcgcggtcaa
     5641 cggtgacgcc agtcatccgc cgtagctctc gttcaagact gtcgagcaac gacaattcca
     5701 acggtgtgac gcggcctaga aacgcttcgg cgtagtttgg cgcgggcaca aaattacggc
     5761 gtactggttt cggcaacgat ttaatcagag caatcaccag ttcgcggcgc agaccgggga
     5821 tttgccattc aaacccgctc tcttctacct gattaagcag cggcagcgga atatgcacgg
     5881 tcacaccgtc cgcgtccgcg cctggctcaa actgatagct caaacgcagc ttgagattgc
     5941 cctgatgcca gaagttcgga taatccagct tgctgatttt ttccgcccct tctttgatca
     6001 acatgctctt ttcaaagttg agcaaatcag gcgtttcgcg gctgactttt ttccaccagc
     6061 tatcgaagtg acgagcggag attacatcgt ggctgatgcg ctggtcgtag aactcaaaca
     6121 gcgtttcgtc atcaaccaga atatcgcggc gacgtgattt gtgttccagc tcttccactt
     6181 cggcccgcag tttcaggttt tcacggaaga atgcgtgacg cgtctgccag tcaccttcca
     6241 ccagagcgtg gcgaataaag agttcacgac ataacgccgg atcgatctgg ctgtagttga
     6301 ccttacgcgc agcaacaatc ggcaaaccat aaacagtgac tttttccgtt gccatcaccg
     6361 cgccctgcgc ccgttcccag tgcggttcgc tgtaggtgcg tttaatcaaa tgctgagcaa
     6421 cgggttctac ccattcaggg tcgatacgcg cagcaatgcg cccccacagg cggctggttt
     6481 ctaccagttc cgccaccatt acccatttcg gcggcttttt gaataaacca gaaccgggaa
     6541 agatggaaaa acgcgcgtta cgtgcgccgg tatattcttg tttatcggca tctttcatgc
     6601 cgatatggga aagcaaaccc gtcagcaacg caatgtgaat ttcgcgatac tccgccggtt
     6661 cgctgttaac cggaatgcca agttctttca ccacctggcg caactgggtg tagatatcct
     6721 gccattcgcg cacgcgcaga tagttgagat aatcggtacg acacaggcga cggaaggcgt
     6781 tagaagaaag cgccttttgc tgctcgccga gataattcca cagatttaca aacgcgagga
     6841 agtcggattc tttgtcgtgg aagcggcgat gtttttcgtc tgatgcctgc tgtttatcca
     6901 tcggacgctc gcgcggatcc tgaatggaga gcgcggacgt gataatcatc gcctcacgca
     6961 cgcagccatg tttttgcgct tccagcacca tacgcgccag acgaggatcg acaggcaatt
     7021 gcgaaagctg gcgaccgagc ggcgtcagtt tataggcgct ggcctgttca tcagtggtga
     7081 tcgcgcccag ctcttcgagc agacgcacgc catcctggat attacgttta tccggtgctt
     7141 cgacaaatgg gaacgcggcg atatcgccca accccagcgc ggtcatctgc aaaataaccg
     7201 aggccaggtt agtacgcaga atctccggat cggtaaactc cgggcgcgag aggaaatcgt
     7261 cttcggaata gagacgaata cagatccctt cggacacacg accacagcgg cctttacgct
     7321 gattggcaga cgcctgagaa atcggctcaa tcggcaaacg ctgcacttta gtgcgatagc
     7381 tgtagcggct gatacgcgcc gtacctgggt cgataacgta tttaataccc ggtacggtca
     7441 gcgacgtttc cgcgacgttg gtcgccagca caatgcgccg tccactgtgc gactggaaca
     7501 cgcggttctg ttcgctgttc gaaagccgcg cataaagcgg caagatttcg gtatggcgta
     7561 agttcagctt gttcagcgca tcggcggtat cgcgaatttc ccgctcacca ctcataaaga
     7621 tcagaatgtc gccagggctt tcctggctca gttcgtctac ggcgtcaaaa atcgcctgca
     7681 gctgatcgcg ctcggtgtca tcggcttctt caacaatcgg tcgatagcgc acttccaccg
     7741 gataggtccg accggagact tcgataatcg gcgcattatt aaagtggcgc gaaaagcgtt
     7801 ccgggtcgat agtcgcggaa gtgataatga tttttaggtc aggacgccgc ggtagcaact
     7861 ctttcaaata gccgagcaga aaatcgatat tcaggctacg ttcgtgcgct tcgtcaataa
     7921 tgatagtgtc gtactgcatc agcaggcggt cttgctggat ctccgccagc aggataccgt
     7981 cggtcatcag cttgaccatc gtgttatcac taacgtgatc actgaaacgc actttgtaac
     8041 cgatgcaacc gcccggctcc gttttcagct cttccgcaat acggttcgcc accgttcgtg
     8101 ccgccaggcg acgcggttgg gtatgaccga tcagcccttt aatcccgcgc cccagttcca
     8161 tacagatttt cggtagctga gtcgttttac cggaaccggt ttccccggcg acgatcacca
     8221 cctggtgatc acgaatcgct tcgagtatgt cctgcttttt ctgactaacc ggcaaattgt
     8281 ccggataagt gatttccggt cgtgccgctt cacgcagcag gactttccct gccgcatggt
     8341 caatctcttt cgccatctcc tggaaaatgg cctgttgtgc atcaggattt ttaaccttct
     8401 tcacgccgtg cagacggcga gaaaaacgca gtctgtcgcg cagcatcagc gaatccagcc
     8461 gctgctgcaa ggccgtaaag gtcaattttt gttgttctgt cataacgtta gtgggcagta
     8521 gtctgcctga ttaattttct tttaaatgat agatatagag taccacatcg cggctttcta
     8581 tgcgttgttc aataaattcg aacataggct tcgatttatt gcgctatctc tgtggcagga
     8641 ttgtgaataa agtgtcaaca agcaacgggg catcgcccat tcaaacatct ataaggaaac
     8701 accatgagca aggtattagt tcttaaatcc agcatcctgg cagggtactc tcagtctaat
     8761 cagttgtccg attattttgt tgaacaatgg cgcgaaaagc actccgctga tgaaatcacc
     8821 gttcgcgacc tggctgcaaa tccgattccg gtgctggatg gcgaactggt tggtgcgctg
     8881 cgtccgagcg atgcaccgct gactccgcgt cagcaggaag ctctggcact ttccgatgag
     8941 ctgattgccg agttgaaagc ccacgacgtt atcgttattg cggcaccgat gtataacttc
     9001 aacatctcga ctcagttgaa aaactatttt gacctggttg cacgcgcagg cgttactttc
     9061 cgctatacgg agaacggtcc ggaaggcctg gtcaccggta aaaaagccat cgttatcacc
     9121 agccgcggtg ggatccataa agatggccca acggatctgg tgacgccgta tctgtccaca
     9181 ttcctgggct ttatcggcat taccgatgtg aaatttgtct tcgccgaagg gatcgcatac
     9241 ggtcctgaaa tggcagcgaa agcgcagtct gacgcgaaag cagccatcga cagtattgtt
     9301 gctgaataac tggacacgat cttgatgtgg actgtagata ttcagtccac atctcaatcc
     9361 gcttacctgt tttcccataa tttcagcatc gctttgtgct cttccttaag cacaatccgc
     9421 gagcgtctgg ctcgaatata actaatcgct tcatcaacgg ttttacaatg tccgtaacat
     9481 aacagccatg ccgccaccac cagcgcactg cgcgataatc ccaacgcgca atggaccaga
     9541 acgctgcctt gctcttcgcg taatgtttcc agcatcgcca cggcctgtcg gagctccccc
     9601 tcttccggaa ccaccagatc cagcatcggt acacaaaaat agagttgatc ttttgtggct
     9661 cgtccgcgag ggaattcaaa ggtgacgtcc agaacagcat tctgtgccgg aatatgttgt
     9721 ggaaacgccc ctaaataaac accagcagta attttgctca ccgactccag gcgaagacaa
     9781 aaccaacgca tagacagcca catcccgatg cgccagggca atgtcagcca gtaaacggcg
     9841 ggcgggagtt tcccctaact atctttccct gttgttatcg cgccaagccc accgtaccca
     9901 cggccaatga tgaatagcga taatactggc caacataacc agactgacca ccataactga
     9961 agcattatca ttagctccat caacacaatg cacgagcacg cgcctacgac atatggcagt
    10021 gctattttga ttcgacgttg atcaggtttc tgataattcc aacgacggtc gacgggcacc
    10081 atccagtcaa ttaacatacc taccgccagc cctgtgatga catcaataaa atgatgctgc
    10141 caggtcgtca gcgtcgaaat ggcgatgagt aaaaaccatc cgccgcagac tttacgccac
    10201 ctcacagcca gatgctgacg aaagtgacgc caaagtagcc agcagagaat aatatgcagc
    10261 gaaggagact ggttataagg cagatcaaac aattcaagtt gcgaaaataa ccatcctgtc
    10321 accccactca cttcaggacg gataaaacta aacttcagcg gatagagaaa aaaaccgcag
    10381 caggccatta ccgttgccag aataagccgg tggaccagac ggcgctgctc aaatgtcgag
    10441 ctacaaacaa acagcgaaaa tccatataaa agatccagac tccagtaagg aacaatagtc
    10501 cagggaagaa aagggatcgc cgtttcccaa ccgaataact gactggggat atcatggttg
    10561 ttaagatcct gagtcgcggt gaactgatta agaaatccat atgtgaagaa gaaaaacggg
    10621 gccagcaaca ataaccagcc agcgccttgt agcaatacgt tccggcgttc agttaccacg
    10681 acgcaccgcc atcgaaaccg taaaaatgcc ccactcatca atccgttgtg tgcatttatc
    10741 gaatccggca tcatgcacga gcgaatccat ctccccttga gaacgcacgc gcattaccca
    10801 cggtttacca tctttatgac tggttaacac cccggcaatc agctccagtt gtgggtgcca
    10861 cggctgcccg gtgtagatga gaatgccacc cggatcgatg gcattggcaa gacctgcgag
    10921 tgagtttttt acctgctcat tttcgggaaa aagctcatac agtccagaga caatcgccag
    10981 cgtaggccgc ggagttaacg cgctgagctc ctccgggtta aacgcatcgc cctgttcaaa
    11041 acgcacccgc ccagacattc cccgttgggc aatcatctct tgcccctgtg caacatttaa
    11101 ctcactgtaa tcacgtaaga aaatatcgct tacggcaggc tcgttcgcca gcgcatccag
    11161 tacatagcgt ccatgccctg cggcaatgtc aaccacgcgg acggctaaac ctttagcgtg
    11221 gagatcggca acggcttgtt taatcagcat ttgtaaatgg gttttgcgct ggcgaatacc
    11281 gcgccagcca acactgttca ggtagatttt gtcgactaaa cgcccgaatg cgttactacc
    11341 ttgcggttga ttttgataga cataatccag cgaactgcct gaatcaaagc cggtgctcat
    11401 tccgagatgc aggcccgcag agtgcgcccc gaacaatttc atcgctttac gcataaagcg
    11461 atacgccaaa tcaaccggcg ataatggcac gggtccacca gaaagtagcc gccatcgatc
    11521 cgctgatggt cctgtgcagt cttcatgctg ataatctaat ttttgcgatt tgttagcata
    11581 taagtgacta ataaagcttt gcattttttc aaataccagc gccctgttct cttcacccaa
    11641 cgtgtcgtga taaaagcctg gcagcagatg cagctctttc agagggctac gtaaacgctg
    11701 ataaaaatca atctgcggtt ggcggtgcac cacatagtca tcgcctgata tcagaagttg
    11761 cgtggggagc gtaatcgccg ccgcatcgct aataatacgt tcagacgttt tatagagatc
    11821 tagcaagata ttgacggcaa tcgcccttgt gatcagcgga tcattattga aactagccac
    11881 ccgttgccga tcgtgggtca aatagcgtcc tttcacatag gaattaataa aaaacagacc
    11941 acgcagacga tgccataacg ccagcgcagg acgtgccagc ggcacataca atttaacctt
    12001 aaaggccgga gaagccagca ccagcccgcg aatagcaggc gcataatcat gaacccatgt
    12061 agcaaccatc actgcgccga cgctttgcgc gatcacaacc acctcttcca gtccgacctg
    12121 gctgtcgctg gcagcaaaac ggacaaattc atcgacatct tgcactgaac tcgcaagaga
    12181 cggactataa ccacgcggcc ccgaagtttg tccatgccct cgggcatccc atgcataaaa
    12241 agcagtatct ggcatcgcca gttcatcaac gagatgttgt agacgaccag aatgttcatg
    12301 accgcgatga aataagacga tgaccttttt cgcccctggc tgtaaagtgg gccagtgccg
    12361 ataaaacaac gctgtattat cactggtggt aaaaaaatgt tccccaggga tgcgtgaatt
    12421 ttccattctc tctattttcc ttcagtaaca gcaatagcga ataaaataaa aaaataccgg
    12481 agcggtgaaa attaatgagt caatcctgtc aaggagtccg ccatgtcctg gtaatagttt
    12541 tccactatct ttaacaccaa catctcgttt gatggctgac atcactacat cgccgcaaaa
    12601 accactaata ccaattaaca aacccgctaa taatgcctgt aatgtatcta gcggtgtcag
    12661 taacggtcca ataatcagtg acacaatcat ggtagtgatt acgcccccca ccagaccttc
    12721 cagcgttttt cccgggctga ctttggggac cacttttctt ctgccgcagg atttccccca
    12781 taaatactgt gcaatatcgt ttgactcggt taatgccaaa agaaataaca ccagtaacgc
    12841 ctcgccctgg atatttgtgg ttggcaacat taataaccag gcggcatgac tcaaggcgaa
    12901 aacggtcatt atccagccac aaaaaatggc gctcacggta tgcagaaaac cggagggttc
    12961 acccacaaaa actcgccagg tggctaatat cagaaaccca aacaaaggta taaataacag
    13021 aaacagctca aagcaattta aaccgataag caaatagtta agaggaataa cccaatataa
    13081 ccaacgcgga aaatgtacgg atataagcgt acaatattct ttcaatgcca gaaaacttat
    13141 cagcgcaaaa aacgtcaacg tcacccagcg tggacctgaa atcaccagtg aaaaacaaat
    13201 gacgatgccc caccaggtac gaatacgtaa tgtaacttca tccccttttc tctccggtaa
    13261 ccgccatacc agaaaacggt ttatcagcgt tgctaaaatt aacaacgcga aaagtgttgc
    13321 cagagatttt tccagcatta tttttcagcg ttcatcagga gcacactccg acaacgatta
    13381 atcgcagtcc agagaagcag aattgaagca atgctccata aaagattatt ccattgcatc
    13441 cactgtggat aaatggcaac ggccagcacc cacaacccaa atattaacgc gcgatcgctt
    13501 ttgccaaatg gaccggcata actgcgaata ccgttaatcg tctgcgcgag taaaccgcaa
    13561 aactcagtca atatggtgca aaataacatg agtattacga gtgatgcgtt actttccggt
    13621 aaaaataaaa acggtaaata aagcgcaata tcggaaataa catcgccagt ttcattcaaa
    13681 atcgccccaa gacgtgtttg ctgattgcac tcacgcgcca tcatgccatc cagcgcattg
    13741 agtgccatac ggacaaaaag cacgatgggc aatagcagaa acatgccatg atatggcacc
    13801 aacgccaaca acaaaccggt gagaagagaa agtgccaacg cggcaagggt gatctgattt
    13861 gcagacactt gccgcttatg aagccaaaac atcgtcggcc ttaacagtga ctgaaagagc
    13921 ggttttatct ggtagagcgt cattatcaat ccttgataat tctgtttgcg ctatggtgca
    13981 tcaattgccc tggcagagat atcgcaacac gccgcttcat taaaaattca gaatatatca
    14041 aggtattggg gctcactgtc aggaatgatt atccccaatt aatcaaatag ttaaaaatat
    14101 tacgctgtta ttcaatgcaa ctcatcaagt tttctcatcg cttgttcccg tattgcgcgc
    14161 gtcttaccgt taagcgccag ctggcggact aaatccagtt cagggttaac aagctctaac
    14221 gccagcaaag gatcacgttt cagcctcgcc tttaatacat caactggaaa acggggatta
    14281 ttcatcgcca caatccacca ctcgggatca atttctgccg ccagtactgc aggcggtgtg
    14341 tggcggttgt taagtgcgcc gaacttgaaa aatgtgagat tgcttttcgc cagtgtactc
    14401 caccatatac gatccggttg ttttgccagt acatccagtt gtgctaacgc atcgggttct
    14461 agtgcataat aaacacgtaa cgcttcgtta acctgcgcac gggaactgtt ttcctgcctc
    14521 agaaaaaact tcgcccaaat atcatttcgt ggcgtgtttt gctgtaattc ctttaagcct
    14581 tgggagagtt cgcattatgg caagatcaaa tgtcgcaatg tctcgtcctt caaatgcagc
    14641 aacgttgcct tcatccagca tttgcaattc ctgcaataat tcaggagaag acttagggcg
    14701 aaacagcgct tcttttttca tctgattaca gacattccct gctatgcgaa tattgcttcg
    14761 cttaaaaatt tctttcagag catcttcatc atttattagg ccccaataat atgcatcagg
    14821 atttattatc atcagcgctt ttttgacatt ttcagtgtca tttttgtcac tcgcacggac
    14881 catgaattca ctatttgtcg aagatgcaac aacaggtgca attatgtcat tgataaatat
    14941 cccgcgcgca ttaatacttc ccttaatcgt agacggatta tcgtaatatg agtcgataaa
    15001 taccagtcca cttgcattta tcgaaccggt aattaacagt ggtgaatccg taactatatt
    15061 cgttgccgtt acgttacgtt acgttacgtt acgttacgtt acgttacgtt acgttaccga
    15121 gaataaccca tggattagaa agagagaaag tggaaatatt acctttgact gtcaaattgc
    15181 caagaacaat caatttttga ttactctcta tattaccttc taccattaaa tcgccacgat
    15241 gcactttcca gtccgatgca gataaaccga cggggacaag attcacgcta ctgttgatag
    15301 cttgttcaac ttgttgttta tcttcagcgg tagccgcaga aaaaaatggt tcaatatcaa
    15361 ctgcatttac aacacatcga agttccgcct gtactggaag actcagcata aacaggggaa
    15421 gtaaacctat ccagctgcaa atcctgggct taaacatatg cactccatgc cattaaaaaa
    15481 gaaacaaaag ggaaatgcat ctttcccttt tgtatattcg ttattctcgc gaaataccgt
    15541 ccaacgtaga caacacttcc tcagaaagat gcaatttttc agcagccata ttctcccgta
    15601 aatgcgcaac agaagacgtc cctgggatca gcaaaatatt cggtgaacgc tgtaacagcc
    15661 acgccagcgc cacctgcatt ggtgttgcgc ccaggctcgc tgctacatcc gaaagtgtgg
    15721 aggattgcag cggcgtaaag cccccaagcg gaaagaacgg cacgtaggca atgccgtcac
    15781 gagccaaagc atcaatcatt gcgtcatcag cacgatgagc aatgttgtat tcgttctgca
    15841 cacagacgat ttcggtaatc ttacgcgcct ctgcaacctg cgtcggtgta acgttgctca
    15901 gaccaatatg ttttaccagg ccttgttgtt gcatctctgc cagcacggtc aggctggctt
    15961 caatcgatcc ttccgctggg ccatgaccat cccccatcat aacgcgcagg ttaaccacat
    16021 ccagtgcgcc cagcccgaga ttacgtagat tatcgtgcac cgctttttgc agctctgccg
    16081 gagaaaatgc gggcaaccag gatgcgtcct ctccacgccg cgcaccaatt ttagtgacaa
    16141 ttgtcaggtc gtcagagtaa ggataaagcg cttcgcggat aatctgattg gtgacgtgcg
    16201 gaccataaaa gtcgctggta tcaatatgat tgacgcccaa tgccagcgcc tcacgcagca
    16261 cggttatagc gacgtggcga tctcttgggg ggccaaaaac cccaggacct gccagttgca
    16321 tcgcgccata accaagacgg ttaacggatt ttgtaccgag agtaaatgta ttgctgctca
    16381 tcgcaccctg ccctgtaata aaacggatta agaaaaatag tatagaaacg aaaagagata
    16441 aataatgcga aggcgtaaac aagattactg gatgagttgc tctattgccc tgaaagacac
    16501 caaaacaaaa cgcgtcaagt accgcatccg gcaataaaaa aaccgcaacc tgtatcagcg
    16561 ttgcggttta ggtatcacat gctaaaaatt agaacgtcca gcatcaggac gttactgttt
    16621 cggtcgcaga ttctcatcaa ataccaggcg ctgcctgcca ggttcaactt cccgcaatgg
    16681 ctcaacttga tgcaacgtct gcttactgcg tgtcaccagc acctgatact catgcgtgcc
    16741 ctgctttttc catgccagtt cctgctcact aagttcacga atcgcttttg ccgggctgcc
    16801 gacaatcagg taattagcag gcatttctgc tttggctttc acaaatgcgg aagcgccaac
    16861 aatgctgttc tcgccaatca ccgcaccgtc catcactacc gcgttcatac ccactaatgc
    16921 attgcggtgg ataatgcagc cgtgaaggat agcgctatga ccaatatgcc catcttcttc
    16981 cacgacagta tcctgatcgg gaaaaccgtg cataacgcaa ttatcctgaa tgttcgcgcc
    17041 atctttcacc acgatacgac cgaaatcgcc acgcaggctg gtatttggcc caacgtacac
    17101 gcccttgccg agaataacat cgccgatcaa taccgctgtc ggatggacaa aactctcttc
    17161 tggcacaaac tgagtcagac cgtctatctg ataaattggc ataacgcctc ctgttcaata
    17221 ttcaagccgc caaaacgttg aaaatacagg cttcccggcg caggcaattc accgaccgag
    17281 gtttcacctt tttcactaac gaacgctaaa gcagcaggcg ctacgcgctg ataaagtttg
    17341 ccgataaagg tttggtagtc gcgcaatata tccgtaaccg tcgtctcgat ttttgtgcag
    17401 atgcgattcg ccatgccgca gatgatgaaa aactggcagg cattggcttt cattggggat
    17461 tttccgacca gagtcatttt tcaacggtat ttaagcaacg ctttgggatg acgccaggcg
    17521 agtatcgacg taaattccgc taactaattc gcgttgcatg tacagcaacg cgaattagtt
    17581 aaaacagata tttgactatg catttttcag ggcgaaaggc aatttgacag gaatttttga
    17641 atattcacgt tgtttccgga aaatagatta atcgtcttct cctgtagcgt tatcccgata
    17701 ttgcggtttt gctattaaaa ttttcgcacc catttaccgt ttatcccttg tacatattcc
    17761 ccgggtttgg cccgttctac aagcttttgc ccggcgagtt tcgcgatatc atccaccgac
    17821 acattattct gctttgccag ttgttgatag ctcgctttac gttcggcatt gatgtcggtt
    17881 actaatttct cagtttcagc atctgttttc agcgcaacca gataaccata aaatgtttcg
    17941 cctacccgcc cctgagttct ggcttcatcc agagtcaatg ccattacatt gctgcttacc
    18001 aagccaacaa gaaatgcaca aagaagtaat gttcttttca tcatcacctc aaaaaagatc
    18061 gctacgggtc tcaagcagct cttcgacatc tttgtctgcc ttgatgatga tctcatgctc
    18121 aattttaacg ttcatattga tagtgatcgg ttccttaggt gcagcgactt caatgcgagg
    18181 cgtacagcca accagcataa aagatgacgt caacgcagcc agtaaaattt tcattgttta
    18241 tcctcacact cttttccttg cggacagtca tttcccggta gatgcgcgtt ctgctccagc
    18301 catgcctgga gattatcgcc aaagcgtaaa ctgcgccaca gcgtaaaaat attctcttca
    18361 tgatggtaat taagatttac cgtaccgctt ttaccatcaa cgcgactggt acctgtaata
    18421 ttggcctgca tggttaataa accgagatta tctaaattaa tttttgtcga tgaacggcta
    18481 atttccatat agcgcaacca gttaattgct gaacccgcgg tcatattgtc tttcaccacc
    18541 gcatctgcgg tgtctttatc cagccgcaag gtcattggcc cgctatttgc cagccagccg
    18601 tctttcacta tccatttttc gttgtttagc cataacggca atgcaccact aaatgccccg
    18661 gacatggcga attgtttcgg attgacggcg ctaaccagtt cgctggatga gagattattc
    18721 agccgcaaca gcgccgagtc atgttgcggc atgcgtaatt gtttcatcag cacgttaccg
    18781 cctaacacat cgacgctaac atcagtcaac agcaatggtt cgtcttcact ccacggataa
    18841 cgcccttgca aatcagccgt aatatttttc gctgtcacca gattaatcac ttcggcaatt
    18901 cgcaacgtaa cggggccgcg agtccccaga tgccaggctc catcggcaaa acggaaaggc
    18961 aggacaaaat cgacaccgtt aacctgatta tctggcatcc aggcactacc gcctttcaac
    19021 acaccgtgcc ctcccgcgcg gaatccttgt tcaggcgcag ctgaaaatgc aacctgagca
    19081 tacagttcgc catcgcgtaa gttcatcttc cagtcgggtg gcaccagcgg ctggaataca
    19141 gtcagtgatt gtttaggcca ccaggcgtta ccgcgcagac gaataccgtc ccagcgacca
    19201 tttacccgaa ccgggccaat ctcaccagca tgcaaatcgc ctttaaagag gaaatacgtc
    19261 ggatcgcgcc catcgacact aaattttaag gttgatggcg gtaacacact gccgccagtg
    19321 aacagcgttt gcccggcgtc cagtgacagc gcgccgctaa aggagggatg ctgtgcgtca
    19381 cgtacccaga cgacgggctt gtcgagaatt aatcgcggct tttctaccgt catcgtaccg
    19441 tattgtaact ggtcgaaacc ggtggagaga tcggtcagcg taatcgtgct gtcatgccac
    19501 tcaccctttc ctgcgacatc ccaggtggca ttcatcggtg taaaactgcc ttttccccag
    19561 tagcgccact gccagcggcc agcgtcaggg agaaaatcat tcgccagtcc atccatatgc
    19621 agcacgaaat cgcccagttc attttcatgc gcctgcaaaa tggcctgcaa atgtccgtca
    19681 acaccacgtt gggtgacttt tacacccgct aaaggccagc ggatttcatc gatgtccagc
    19741 gaatcgatga ctcttccctt cgaacgtaat aacgcgcctg gctcaaaggc cagcgttggg
    19801 tcagtcagac ttccgcttag ctgcgcaggt aaacgggcat ataaaatgag gtccgcctgt
    19861 ttcgcttcac cggtcagctg caaaggcagc tggctgttat ccatacttaa ttttcctggg
    19921 ccaaaattaa gcaccgcgtt gcccttaccc gcttgcccct gggtcagcac actcagtcgt
    19981 ccgctgacca gagcgttctc gagccctgcc tgccagttgt cgactttgac acccagtcgt
    20041 ccactcaaag gaaaacctgc atacggccag ctccagcgac catcgcttac ggtcaattgt
    20101 tggcgagtaa tttgccacgg cagatcgagc aacggatcgc cgttatcccg tgccaccaca
    20161 atcaattgcc cgctattttc ctgccagtcc agctcggcat ccaccagtga aggctcctgc
    20221 ggcaagttta acgtcgcagt agcatgacca cttacaggaa gtccatccgg cactagcggc
    20281 atagtaaatt cccccaccag tttaaccggc ggctgatttt caaacgcgac gacatccagt
    20341 tcgctgactg taagttgttg ccctttcagc tggccttgaa atttaacttt ttcgccctga
    20401 taacgcagtt gctggatatc ggaggttaat gcgagagaga gttttccctg ccattcctgc
    20461 cagggagaaa aaatcagttt atcgatattg atccaggtgt taggcaacat ggcctgccac
    20521 tgcgcgaggg tttttggagc ggcaggcgat tgctccgtct gcggcaattt cgccagacaa
    20581 gcagaatcaa gttctaccgt gccgacgttc aataaccagc ggctgggatg tgaaaggctg
    20641 gcgttggtga tatgcgcaag ctgacaatct cccaccagat aacggagatc gggaatgatt
    20701 aaacctttac gcgtaatgcg tgggctttca tctaatgcaa tacgtgtccc gagcggtagc
    20761 cagatgcccg ccagcgtggg aacccacagc ccgagcgtca tcagcagtgt caacggcaca
    20821 agaataatca gtaataatag cgcgagaacg gctttatatt tacccagcat gggtagttaa
    20881 tatcctgatt tagcgaaaaa ttaagcattc aatacgggta ttgtggcatg tttaaccgtt
    20941 cagttgaagg ttgcgcctac actaagcata gttgttgatg aatttttcaa tatcgccata
    21001 gctttcaatt atat
//
LOCUS       DAZKGF010000086        20801 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZKGF010000086 DAZKGF010000000
VERSION     DAZKGF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.086"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..948)
                     /gene="waaO"
                     /locus_tag="ACHOQV_002916"
                     /gene_synonym="rfaI"
     CDS             complement(<1..948)
                     /gene="waaO"
                     /locus_tag="ACHOQV_002916"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFP9631257.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKLIEAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNF"
     gene            complement(964..1761)
                     /gene="rfaP"
                     /locus_tag="ACHOQV_002917"
     CDS             complement(964..1761)
                     /gene="rfaP"
                     /locus_tag="ACHOQV_002917"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFP9631258.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKKEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVASLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(1754..2878)
                     /locus_tag="ACHOQV_002918"
     CDS             complement(1754..2878)
                     /locus_tag="ACHOQV_002918"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9631259.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQFSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(2875..3933)
                     /gene="rfaQ"
                     /locus_tag="ACHOQV_002919"
     CDS             complement(2875..3933)
                     /gene="rfaQ"
                     /locus_tag="ACHOQV_002919"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFP9631260.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            4346..5623
                     /gene="waaA"
                     /locus_tag="ACHOQV_002920"
     CDS             4346..5623
                     /gene="waaA"
                     /locus_tag="ACHOQV_002920"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFP9631261.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATSLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            5631..6110
                     /gene="coaD"
                     /locus_tag="ACHOQV_002921"
     CDS             5631..6110
                     /gene="coaD"
                     /locus_tag="ACHOQV_002921"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP9631262.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(6149..6958)
                     /gene="mutM"
                     /locus_tag="ACHOQV_002922"
     CDS             complement(6149..6958)
                     /gene="mutM"
                     /locus_tag="ACHOQV_002922"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFP9631263.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCEKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGESCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(7056..7223)
                     /gene="rpmG"
                     /locus_tag="ACHOQV_002923"
     CDS             complement(7056..7223)
                     /gene="rpmG"
                     /locus_tag="ACHOQV_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9631264.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(7244..7480)
                     /gene="rpmB"
                     /locus_tag="ACHOQV_002924"
     CDS             complement(7244..7480)
                     /gene="rpmB"
                     /locus_tag="ACHOQV_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP9631265.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(7697..8365)
                     /gene="radC"
                     /locus_tag="ACHOQV_002925"
     CDS             complement(7697..8365)
                     /gene="radC"
                     /locus_tag="ACHOQV_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9631266.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            8537..9757
                     /gene="coaBC"
                     /locus_tag="ACHOQV_002926"
     CDS             8537..9757
                     /gene="coaBC"
                     /locus_tag="ACHOQV_002926"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFP9631267.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRERGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            9735..10193
                     /gene="dut"
                     /locus_tag="ACHOQV_002927"
     CDS             9735..10193
                     /gene="dut"
                     /locus_tag="ACHOQV_002927"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP9631268.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            10300..10896
                     /gene="slmA"
                     /locus_tag="ACHOQV_002928"
     CDS             10300..10896
                     /gene="slmA"
                     /locus_tag="ACHOQV_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFP9631269.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(10933..11574)
                     /gene="pyrE"
                     /locus_tag="ACHOQV_002929"
     CDS             complement(10933..11574)
                     /gene="pyrE"
                     /locus_tag="ACHOQV_002929"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP9631270.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(11640..12356)
                     /gene="rph"
                     /locus_tag="ACHOQV_002930"
     CDS             complement(11640..12356)
                     /gene="rph"
                     /locus_tag="ACHOQV_002930"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP9631271.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            12483..13346
                     /locus_tag="ACHOQV_002931"
     CDS             12483..13346
                     /locus_tag="ACHOQV_002931"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP9631272.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNVE"
     gene            13534..14805
                     /locus_tag="ACHOQV_002932"
     CDS             13534..14805
                     /locus_tag="ACHOQV_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9631273.1"
                     /translation="MTKAKLTDIQIKAWIKAGERFDARADGNGLYLCFPEKYNAPFWR
                     YRYRMAGKQRAMHIGSYSDLSLSKARATVKELAARVALGHDVAGEKQARKAAALARIE
                     EEKNALRVSELAAEYYERQILPRWKHPDILRRRIDKDINPQIGSLKVEDVKPRHIDAM
                     LQRIIDRGAPTVANDVLRWTKRIFDYAIKRHALETNPAAAFELSDAGGAEKSRDRWLT
                     RDELIHFFQAMRTAKGFSRENELTMKLILCLCCRKMELCAAQWDEFDLDSAVWRLPAE
                     RAKNGDAIDIPLPPPAVAWLRELHSISGGSRWVLPARKMQQRMIPHIQESTLPVALAK
                     VRREMPGVPGFTVHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFNER
                     RHALELWADLLVALEQGDEYNVTPITKAIAK"
     gene            14901..15854
                     /locus_tag="ACHOQV_002933"
     CDS             14901..15854
                     /locus_tag="ACHOQV_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631274.1"
                     /translation="MYENTYPIDTAASILGCTQKKILTEWAKGTIKIALDFGPESSIN
                     RQSYTDYDKWGYPAKPWNIASISAPATNDYFPPELLQPEEGEKLRLFPNDNEPESILI
                     WRNVYHQYSQSGFYFDSNHSDAQVHDGKLMVVSDVLLYGLWTVPYAEFSRVHAQDTFT
                     LKPYTDTPSLSKVTATFTPRGGSRRINAPEKKNLRITDSEMMVMQKILAQRPGYQRTE
                     QPTDDRNQEKHDIHGGVERFALEREKVLAAALYVAYHFPKDVGKTLKSHAEAIEKHGY
                     LFWDGNPTPTPDAGRIAKILSDARRLPNEWKILGGNAKAKR"
     gene            16024..16230
                     /locus_tag="ACHOQV_002934"
     CDS             16024..16230
                     /locus_tag="ACHOQV_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9631275.1"
                     /translation="MDTLPKSGYMRPAQVINHFQISESTLWNWVKKGKFPSPDKLGDG
                     VSRFDVSAIRQWLADRKGTTSTRN"
     gene            16252..16857
                     /locus_tag="ACHOQV_002935"
     CDS             16252..16857
                     /locus_tag="ACHOQV_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9631276.1"
                     /translation="MKMPERPATGPGRSHAQNNHHTPHRMGGNRKYCLPAIAGGRYIS
                     PAGVNPPAGRGNPFNEQKATQTRPASFFVSCHRATMNSGKARTVSMVALVGQPKGWPV
                     SFCAGISTPASVTAPYERGNSGGDSLNLQKEAARMATTPTPLHPEFTFHFLAVRRSDL
                     NDRPHRESVNAPDETTARRMLARDFVLLFAGRVPAQGVSHA"
     gene            16850..17314
                     /locus_tag="ACHOQV_002936"
     CDS             16850..17314
                     /locus_tag="ACHOQV_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9631277.1"
                     /translation="MLKNMLIASSFGLYDAGVTHMVTGFSSLNNIADSRLHSDMRFFC
                     VRKPTHTRIMAGRDGGAFALAGFYVASLLTPLRLATPFSSVVARLLTSNIGAFHMAVF
                     ARPYFVWRFMQCQEKQTSLFTVTAATEREARAQLPHAHMIFVARIRQGARHA"
     gene            17307..17603
                     /locus_tag="ACHOQV_002937"
     CDS             17307..17603
                     /locus_tag="ACHOQV_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631278.1"
                     /translation="MRDCLRESMKAAMSSMPDEESHWILRVDADWHRVNLLAGIAFVG
                     KALEGSQLRENPITYSRDEICQLAGFLQTAPALIGCMAELMECYDQQAGEVSHV"
     gene            17596..17910
                     /locus_tag="ACHOQV_002938"
     CDS             17596..17910
                     /locus_tag="ACHOQV_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631279.1"
                     /translation="MCDMYNTRIPELLVAAIKNADAQEARALFDDADYCARKLLEGLI
                     STGRLLSGMGDNLDPSMGELRSLGDSIAVTAELVAGFSEVVEAYNWRCRTGEIREGGQ
                     HA"
     gene            17903..18946
                     /locus_tag="ACHOQV_002939"
     CDS             17903..18946
                     /locus_tag="ACHOQV_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP9631280.1"
                     /translation="MRDIATINAIRSVAAVAAQHAEILVPEWLPEGKRQATEWVCRNP
                     TRADRHAGSFFVSLVDGCWHDFATGDSGGDLVALGAYLWNVRQTDAARIVADRLGLCL
                     PALGKPDVMTREQREAQRVRVQAAEQEAGRLRQREQQQRHQRQKLTACRAFELLGRAE
                     VATPSHPYLMKKRLQPGNLYQSGNDLLVPLYNVCGEVVNVQIISPDGRKMFLRDGQVQ
                     GAFHVMGSFDLMAPEAPEHDVYVCEGWATGAALLQYWNVMAVVCAMNAGNLKHVAMAL
                     RERYGSRISLVIAGDDDRASKGNPGSRAANEAALLAGCYVTFPEWPPGASPDLSDFND
                     LMLWEMEHDPDNH"
     gene            18927..20765
                     /locus_tag="ACHOQV_002940"
     CDS             18927..20765
                     /locus_tag="ACHOQV_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFP9631281.1"
                     /translation="MTRTTTKTPVTSGKRKPPRKKHPKVSPARPGYATYSQHVQVDGG
                     QILKPGLYWHGVRDSTNTDTFICSPLEVIAMTSDSKDENYGRLLRFLTPSGQWREWAA
                     PMEMLAGDGGELRQELLRMGVTIPYKQRQALTEYIMESAPKRRALAATATGWHSPTLF
                     VMPGGVIGDGDVVFQSTEAGSQEYATAGTPEGWRDNVAALCRGNPVAMLAVCASLAGP
                     LLWLLDVDSGGFHFMGDSSSGKSVGALVACSVWGEPRNFKRNWNATSTGLEGLATMRN
                     DTALILDEIGEAPARDIGGLIYQLGNGTGRQRGKVSGMARPVNTWRTMIISTGEMTVG
                     KHMESGGQRIKAGQEMRLLDIPAQRLYGAFDCLHGMALPHNPDNDTERRGAGRKFAET
                     LRGNAATHYGHAGPEFVRWLISRNESDTTSTDSNGQTLADMYAILREQFPVSTGQEAR
                     AAARFTLCALAGEMAITAGILPWKQGEALEAVKAMFAAWSDYRGRGQSEDVKILRAVA
                     GFIARHASRFEGGNTYSEPVSNRAGWWRPGSNGDRVYWFLPEALCEAASGYDLKRIAA
                     SLDDAGAIVDRDTDGKRRYTKRVSVNGEKHRVYAIDQNALLFAIDE"
BASE COUNT         5187 a         5520 c         5270 g         4824 t
ORIGIN      
        1 aaaattatgc ttagcgcaat aacgagcata gtttgagtta actggccgca ttaacggttc
       61 atttttccag ggcgatgctt ctttggcttt gataaaaggt tgcgcacttg gataaccggc
      121 ccagtaatgc cagggttttg tcgggccgac ataatgaatt aatacggttt catcattaat
      181 tggacaaaca aatgattttt ttaattcata atttaaacta aattgcgtat tgtatttagc
      241 ttcaatgagt ttaactttcc ctaacaggat aagattaagg atatcttgat ccatataggt
      301 taaacgggaa acgatggctt tatcagcaag catcgacatc gctttagcag aaacggactc
      361 ctgcgcccac gctagtgtat taattaacag gacacctgaa ttaaagtaac ccttttcaag
      421 ttcattacac tgcagacttt gaccccgtaa cgaccaccag ttagcatcgc gttcagtaac
      481 aacagcagca acgttattgg caagatccat tgttatcagc ggctttaagt ttccctgaca
      541 ggcgatatca gcatccaggt aaaggatctt atcttgttga tcaataaagt aatctgcaat
      601 tacaaaacgg aaatacatgg caatagacca atttttggtc gtcggtaatg ccttaagccg
      661 ttcacaattt actagatgga tctggataca ggtacgatag cttttcgcca attgggctaa
      721 acgctggata tcggcttcag ggatatcatc aataaaaacg tggaaaacaa aacttacgtc
      781 gttgttatgt aacaaaactg acgtgattga aacaccacaa ccaaaaagaa agtttttatc
      841 aatgccataa gcaacatgga atgatgatgc cactgacgcc gctggccttt catcgaagat
      901 gattgtctta ttgatcatct cttgtggatt aaaatagtgg gcactcatat ttctctccgg
      961 aaattacaac gattttcgaa tcgttctttc cctgattttt gtggcttttg cttctgcttg
     1021 cgacagcagt ccttgttcct gcttgagaat gtctttaagc gaggcgacaa aatacacttt
     1081 cataaaccgc cagatatccc gctgagtcag gccaatattc atcgaagaaa aataaagccc
     1141 tataagatct ttatcgcgcc aacgacgtgg aacgcgcgtg cgaagctgcg cccggtgcag
     1201 atcaattacc gaaattttta actcctcttt cttaccggaa aaaggcaagt gcagcaggaa
     1261 atgacagata taacagtcac ggtggttaat gcccgcagca tgcatatcgc gcaccatcgt
     1321 cgcgacacgc ttaataagca tacgctttac gcgaacatct ggagggttag tcgcccagtc
     1381 agcacagtaa tcttccagac tgatggttgg tgtcagatct tcggttataa taaacgaggt
     1441 tctggtgagc ggattaatgc ctttttcgcc gaatgccacc ccatacatag tatcaacgcc
     1501 gacatcccgc agtcgatgaa tcgcattcca ttcgcggtct gcgcctaata ctggcatccg
     1561 caatgagagt aaatttttga ttatctcttt cagggtcgtg ccgcgatgcc atttgagaaa
     1621 atagcttttg cccgccattt caaagcgcag agtacggcga gtttccagtt cacgaaatac
     1681 ctcaccctgc aaagttttaa cttcctcaaa aggatctttt ccgcgccata acgtggcaaa
     1741 cggctcttta agttcaacca tccagaccac ccgttatgat atccgccgct ttctctggca
     1801 gactgtataa atcttgtgta tcagcataat gtcgcgcatt ttccgcccag gcctggcgca
     1861 atgaaaattg cgttaacgct ttgcgtaaaa tctcattcaa tgtttcctgg cggaatggct
     1921 cagcaatagc ctcgccgcaa ttggcgtcga caatataatg cgcatagcca caaacggcag
     1981 ttgttagtac cggtaatcct gcagtaatcg cttccagcag cacaattccc gccgcttcct
     2041 ggtaggcagg atgcagtaat aaatccgccg ccgccattaa ttccgagaca tcgttgcgcc
     2101 ccgagaagaa gtgaacatta ctgcgcacgc cgcgtttttc tgccagcgcc tcaaattttc
     2161 gcggtttatc ctgcccaaca acatatagca atgtgttgtg gcgcagcgaa tccggtaacg
     2221 aagcaagtgc ttcaatggaa cgatcgacac ctttacgcgt gaagtctgaa ccgacctgca
     2281 acaataaata ttgttgttcg gttattccat tcttcttacg gaagatttca cggctattag
     2341 ctggctgctg gctatattta cgatcaggat aaatccccgg tggcagaata tgaaaacgct
     2401 ccgcttcagt ctgataatgt ttctggaaat cggcgatttg cttatctgtc agcatcagca
     2461 gctgtgtcgg cttgccctgt tcgaaggttg cccgctcaaa ggcggcataa tggcgataac
     2521 gtgacgtcag gcgatagaaa aagccttttt cctgcgctac tttctcggca taacaaacat
     2581 cagcggcata ataaacgtcc agccccggca ttttgttgaa tccaacgact ttatcgacgg
     2641 gatgttcgcg taaatgtttt tgcacccagg caaaatactc cgcattgcgc ccgtgattgg
     2701 tatgcgattt aaccggcact ttgatcagtt caaatacatc agggcattcg ccttcccacg
     2761 actgggtata aacccgaaca tgatgacctc gggcggcgac tgtctgagca atacgcataa
     2821 aatcgcgctg caaaccgcca aagggaaaat atttatataa acaaaaagca acgatcataa
     2881 cgacgtacct gtcgtggagg agggcagtaa tttatcgaca gcagcaatga catctgccgc
     2941 cggaataacc gaaagataca tctcatttcg gtcacgctga tcgcgcgttg gcatttcccg
     3001 gtaatctccc gcccagaatt gaatcatgtt atttgaccag ggacgccaga aaatatggtc
     3061 tgtcgcacca aacagcgata tcagcggcgt attaactgca gcggcaatat gcgccggtgc
     3121 ggagtcaacg ccaataaaca gctgcgcatg atcgattaac gcaccaagtt ccgggaaggt
     3181 cacctttccg gccagcgccg ttactggtgg cgtctggcat ccctgcgcaa tttcattgac
     3241 gcaggccaga tcgtctttat ctgggccgga cgtcagaacg acttcataac cacgagcatg
     3301 taaggcatca atcacagcgg aaaacttggc gttgtcccag catttgaaga tttgccgcgc
     3361 cgtaggttgg ataaccacat aattttgtcc aacaccagcg tgatcaagtt cgcgacgcat
     3421 acgtttccag cttgcaggcg ggtaactcat ggttgtctgc ttcaccaacg aatcaactcc
     3481 cagtggtgtc agcacggata agttactttc caccacattt ccaccctgca acggcaccaa
     3541 atgggtgaaa cttttacgcc aaaaagcaga ctgccgatga tgataatcct gggaaatttt
     3601 cacacgggca tttaataagc gcaccagtat agcaaccatc cattgatcgg taagattaac
     3661 gataaggtca tacttattgg cacgtaatac cttgatgaga tggaaaaagt tggcaatttt
     3721 ttctgaggct tttgcttttt tattttttat gccgtagagc gcgttaatct ctggattttc
     3781 agacaggatc gggatggtgt cctgataaag cagcacatcg atttttgcgt cagggtaatt
     3841 ttttttcagc gaactaatga cgggagtagt taataacata tccccatgaa aacgcatctt
     3901 aatgagcaaa attcttcgaa atggcttatc cacaagtaac tctttttgtt gagattatcc
     3961 gattaaggtg aagcagaaaa agcacgctac cgccccaggc tcaacagcta cctgaatact
     4021 gataccgcac tgatattgac gcgctagtgt atcacttctt tcgcggcaat ccgacccgaa
     4081 tgatttttaa gcacattgtt gattttcaat aaagaaatca atcaaataaa atcattagca
     4141 accatattct ttcactgttg ctctgcgatt ccttcctgca cgtactgcag aaatcaatga
     4201 atcgcataaa aatacaaaaa aagtatagat gttcagcaag aattggcggg taaattgcta
     4261 tttaagtcaa aaacgggaaa agtaatggta aagccacagc taaatacata gaatccccag
     4321 cacatccata agtcagctat ttactatgct cgaattgctt tacaccgccc ttctctacct
     4381 tattcagccg ctgatctgga tacggctctg ggtgcgcgga cgtaaggctc cggcctatcg
     4441 aaaacgctgg ggtgaacgtt acggttttta ccgccatccg ctaaaaccag gcggcatcat
     4501 gctgcactcc gtctccgtcg gtgaaactct ggcggcgatc ccgttggtgc gcgcgctgcg
     4561 ccatcgttat cctgatttac cgattaccgt tacaaccatg acgccaaccg gttcggagcg
     4621 cgtacaatcg gctttcggga aggatgttca gcacgtttat ctgccatatg acctgcccga
     4681 tgcacttaat cgtttcctga ataaagtcga ccctaaacta gtgttgatta tggaaaccga
     4741 actatggcct aacctgattg cggcgctaca taaacgtaaa attccgctgg tgatcgctaa
     4801 cgcgcgactc tctgcccgct cggccgcagg ttatgccaaa ctgggtaaat tcgtccgtcg
     4861 cttgctgcgt cgtattacgc tgattgctgc gcaaaatgaa gaagatggtg cacgttttgt
     4921 ggcgctgggc gcaaaaaata accaggtaac cgttaccggt agcctgaaat tcgatatttc
     4981 tgtaacgccg cagttggctg ctaaagccgt gacgctgcgc cgccagtggg caccacaccg
     5041 cccggtatgg attgccacca gcactcacga aggcgaagag agtgtggtga tcgccgcaca
     5101 tcaggcattg ttacagcaat tcccgaattt attgctcatc ctggtccccc gtcatccgga
     5161 acgcttcccg gatgcgatta accttgtccg ccaggctgga ctaagctata tcacacgctc
     5221 ttcaggggaa gtcccctcca ccagcacgca ggttgtggtt ggcgatacga tgggcgagtt
     5281 gatgttactg tatggcattg ccgatctcgc ctttgttggc ggttcactgg ttgaacgtgg
     5341 tgggcataat ccgctggaag ctgccgcaca cgctattccg gtattgatgg ggcctcatac
     5401 ttttaacttt aaagacattt gcgcgcggct ggagcaggca agcgggctga ttaccgttac
     5461 cgatgccacg tcgcttgcaa aagaggtttc ctctttactc accgacgccg attaccgtag
     5521 tttctatggc cgtcatgccg ttgaagtact gtatcaaaac cagggcgcgc tacagcgtct
     5581 gcttcaactg ctggaacctt acctgccacc gaaaacgcat tgaggttgtt atgcaaaaac
     5641 gggcgattta tccgggtact ttcgatccca ttaccaatgg tcatatcgat atcgtgacgc
     5701 gcgccacgca aatgttcgat cacgttattc tggcgatcgc cgccagtccc agtaaaaaac
     5761 cgatgtttac tctggaagag cgtgtggcgc tggcacagca ggcaaccgcg catctgggga
     5821 acgtggaagt ggtcgggttt agtgatttga tggcgaactt cgcccgtaat caacacgcta
     5881 cggtgctgat tcgtggcctg cgtgcggtgg cggattttga atatgaaatg cagctggcgc
     5941 atatgaatcg ccacttaatg ccggaactgg aaagtgtgtt tctgatgccg tcgaaagagt
     6001 ggtcgtttat ctcttcatcg ttggtgaaag aggtggcgcg ccatcagggc gatgtcaccc
     6061 atttcctgcc ggagaatgtc catcaggcgc tgatggcgaa gttagcgtag agtttatgcc
     6121 ggatggtatg ccatccggcg cgcatcaatt acttctggca ctgccgacaa taaaacgttg
     6181 cccgctgcgc atgtttagtc gccacaatcg gcgtaccaca cacccgacac gactcacctt
     6241 ttcgcccgta aacctgcaat tcctgagcga aatagcccgg tttaccatca ctttgcagaa
     6301 aatctttcag cgttgtaccg ccctgctcaa tcgaacgcag caacaccgct ttaatcaccc
     6361 gagctaacaa ttcacactct gccagcgaca gtgatgacgc cagccgatcc ggatggatcc
     6421 ccgccgcaaa cagagattcg ctggcataga tattccctac gcctaccacc agcttgttat
     6481 ccatcagcca tggtttaatc gccgtttttt tcttctcgca cttctgatgc aggtactcac
     6541 cgttgaaatc gtcgctaagc ggctccggtc caagatgggc cagcacgttg tgcccttcca
     6601 gctctttggt ccacagccag gcaccaaagc ggcgcggatc ggtgtagcgc agcactttac
     6661 cgttgctcat caccaaatca acatggtcat gcttttcagg gggaagttct tctggaagga
     6721 tgcgcaggct gccagacatc cccaaatgaa tgataatcca gccctcaggc agctccagca
     6781 gcagatattt agcccgccgc tgcacgctaa gcactggttg gtcgcttaaa cggtagatct
     6841 cttctgaaac cggccagcgc aagcgtccgt tgcgcaccac cgcatgaaga atggttgcac
     6901 caacgagatg cggttctatg ccgcgacggc tggtttcaac ttcgggtaat tcaggcatag
     6961 catctccagg aatgaacaga tgcagtcaat atgggggcag gcagataaca aaaaaccccg
     7021 ccggggcgag gtttttcgtt acatcaaagc aaaaattatt tgattttcgc ttctttgtag
     7081 atcacgtgct ggcgaacaac tggatcgaat tttttcagtt ccagtttttc cggcttagta
     7141 cgtttgttct tcgtagtggt atagaagtga ccagtaccag cagaagaaac cagcttgatt
     7201 ttctcacgaa tacctttagc catgatttat ttcctctaag tacttagtac ttttcgccac
     7261 gggcacgcag ttcagccaga actgtatcga tgcctttttt atcgattaca cgcatacctt
     7321 tagcagatac gcgcagggtg acaaaacgct tctcgctctc aacccagaaa cggtgagagt
     7381 gcaggttcgg caggaaacgg cgtttagtcg cgttcagtgc gtgggaacgg ttgttaccgg
     7441 tcaccggacg cttgccagta acttggcaga ctcgggacat gtctattctc caaaaatcaa
     7501 attagctcga gcttcgtatg gggtattggc gcctcgtcag gctttacagc ccggtcatcg
     7561 cagttctatg tgaactctcg attgccaggc ccaaatgcca aacccgagat tctcaaaggt
     7621 ggcgtagtat acgctgactc agcgatgtgc tcaagtcccg aacagacaaa gatcccgaag
     7681 gatcgcgcat agcgggttaa atccagccgc gttcggcaaa agaaacatac tctccacgcc
     7741 caatcacgat atggtcgagc acgcgtaaat ccatgaactg acaactcttt attatccgtt
     7801 cagtaatgag tttatccgct ttactgggtt cagcacaacc cgaagggtga ttatgtgcaa
     7861 ggatcagcgc cgaggcattt atttttatcg cttcgcggat aatttcccga ggatggactt
     7921 caacatggtt tagcgtgccg gaaaaaagac ggctatgcgt tataaccctg tgttgtgagt
     7981 cgagaaagat caccataaag atctcccgct cctcacccgt gagctggctt tgtaaaaatt
     8041 cccgcgtcat ctccgggctg agtaaagggc tttcttcacg catccgcacg ttgtagtaac
     8101 gccgcgccag ttcggcaatc ccctttaact gggcaaattt cgccacgcca attccatgaa
     8161 caccactaaa ttgttcatat tcagaggtta acaagccata aagagagccg aaattctcca
     8221 gcatctcttt tgccagggtt aatacatctt taccgcgcgt tccggtacgc agaaatagcg
     8281 ccagcagctc gacatccgtt aaggcgctaa taccaaactt cagcattttt tcgcgcggca
     8341 tcaacagctg tgaattgttt ttcaccttca cctcctttgt ggtgctcgca tcctgtcata
     8401 gccgttccgt taaatcgacg gccagttttc aatcctggaa agcgcctcgc aaagtgattc
     8461 tcaggtgaag gcacgacaac aaaagtattg tgataaaatc gccaacttct ggtgtcaccc
     8521 aacaggaaaa atcatcatga gcctggccgg taaaaaaatc gttctcggcg tcagcggcgg
     8581 tattgctgcc tataaaaccc ctgaactggt gcgtcgtttg cgcgagcgcg gggccgacgt
     8641 ccgcgtagcc atgaccgaag cggcaaaagc ctttatcacc ccacttagct tgcaggcggt
     8701 ttctggttat cccgtttccg acagtctgct ggacccagca gccgaagccg ctatgggcca
     8761 tattgagctg ggtaaatggg ctgatttagt gattctcgcc cctgccacgg cagatttgat
     8821 tgcccgtgtt gctgccggaa tggcgaatga tctggtatcg acgatttgtc tggccacacc
     8881 tgcgcctgta gccgtgctcc ccgccatgaa ccagcagatg taccgtgccg ctgccacgca
     8941 gcataattta gaggtgcttg cttcccgtgg tttgctcatt tgggggccag acagtggcag
     9001 tcaggcttgt ggtgatatcg gccctgggcg aatgctcgat ccgttaacca ttgttgatat
     9061 ggcggtagcg catttttcgc ccgtcaacga cctgaaacat ctgaacatta tgattaccgc
     9121 cggcccgacg cgtgaaccac tcgatccggt gcgttatatc tctaatcaca gctcaggcaa
     9181 gatgggtttt gctatcgccg ccgccgctgc ccgtcgtggc gcgaacgtca cgctggtatc
     9241 aggtccggtt tcactaccga cgccaccgtt tgttaaacgt gttgatgtga tgaccgcgct
     9301 ggaaatggaa gccgccgtga atgcatctgt acagcagcaa aatattttta tcggttgcgc
     9361 cgccgtggcg gattatcgcg cagctaccgt ggccccagaa aaaatcaaaa agcaggccac
     9421 gcagggtgat gaattaacaa taaaaatggt taaaaacccc gatatcgtcg caggcgttgc
     9481 cgcactaaaa gaccatcgcc cctacgtcgt tggatttgcc gccgaaacaa ataatgtgga
     9541 agaatacgcc cggcaaaaac gtatccgtaa aaaccttgat ctgatctgcg cgaacgatgt
     9601 ttcccagcca actcaaggat ttaacagcga caacaacgca ttacaccttt tctggcagga
     9661 cggagataaa gtcttaccgc ttgagcgcaa agagctcctt ggccaattat tactcgacga
     9721 gatcgtgacc cgttatgatg aaaaaaatcg acgttaagat tctggacccg cgcgttggga
     9781 aggaatttcc gctcccgact tatgccacct ctggctctgc cggacttgac ctgcgtgcct
     9841 gtctcgacga cgccgtagaa ctggctccgg gtgacactac gctggttccg accgggctgg
     9901 cgattcatat tgccgatcct tcactggcgg caatgatgct accgcgctcc ggattgggac
     9961 ataagcacgg tatcgtgctt ggtaacctgg taggattgat cgattctgac tatcagggcc
    10021 agttgatgat ttccgtgtgg aaccgtggtc aggacagctt caccattcaa cctggcgaac
    10081 gcatcgccca gatgattttt gttccggtag tacaggctga atttaatctg gtggaagatt
    10141 tcgacgccac cgaccgcggt gaaggcggct ttggtcactc tggtcgtcag taacacatac
    10201 gcatccgaat aacgtcataa catagccgca aacatttcgt ttgcggtcat agcgtgggtg
    10261 ccgcctggca agtgcttatt ttcaggggta ttttgtaaca tggcagaaaa acaaactgcg
    10321 aaaaggaacc gtcgcgagga aatacttcag tctctggcgc tgatgctgga atccagcgat
    10381 ggaagccaac gtatcacgac ggcaaaactg gccgcctctg tcggcgtttc cgaagcggca
    10441 ctgtatcgcc acttccccag taagacccgc atgttcgata gcctgattga gtttatcgaa
    10501 gatagcctga ttactcgcat caatctgatt ctgaaagatg agaaggacac cacagcgcgc
    10561 ctgcgtctga ttgtgttgct gcttctcggt tttggtgagc gtaatcctgg cctgactcgc
    10621 atcctcactg gtcatgcgct aatgttcgaa caggatcgtc tgcaagggcg catcaaccag
    10681 ctgttcgagc gtattgaagc gcagttgcgt caggtattgc gtgaaaagag aatgcgtgag
    10741 ggtgaaggtt acaccaccga tgaaaccctg ctggcaagcc agttgctggc cttctgtgaa
    10801 ggtatgctgt cacgctttgt ccgcagcgag tttaaatacc gcccgacgga tgattttgac
    10861 gcccgctggc cgctaattgc cgcacagttg cagtaatatg acgccggatg acttttcatc
    10921 cggcgagttt ctttaaacgc caaactcttc gcgataggcc ttaaccgccg ccagatgttc
    10981 cgccatttct ggcttctctt ccaggtaagc aatcaggtct ttcagggtga tgatagagat
    11041 caccttgcag ttgtaatcac gctcaacttc ctgaatcgcc gaaatctcgc cgcgcccgcg
    11101 ttcctgacga tcaagcgaaa tcaatacgcc agccagcgtc gcgccattgg cctgaataat
    11161 ttccatcgac tcgcgaatcg ccgttccggc ggtgatcaca tcatccacca gcattacgcg
    11221 tccttgtaac gcgctaccga ccagattgcc accttcacca tggtcttttg cttctttgcg
    11281 gttaaagcag tacggcaggt ccaggtcatg atgctccgcc agcgccacgg cagttgtggt
    11341 ggcaatcggg atccctttgt aagcagggcc aaacagcaga tcgaactcaa tgccggaatc
    11401 caccaatgct tcagcgtaaa aacggcctaa cagtgccaga tcgcgcccgg tattaaacag
    11461 cccggcgttg aagaaatagg ggcttttgcg cccggatttc agcgtaaact cgccaaactt
    11521 taacacctgc ttgctaagcg caaattcaat aaactggcgc tgatatggtt tcatgccttc
    11581 gctcctcatc ttacttttct acagacaaaa aaaaggcgac tcatcagtcg ccttaaaaat
    11641 cagtttgcca gcgccgcctt ctgcgtcgct acaatggatt cgattccccc tcgggccagc
    11701 gccaacaagg tgagtaactc ttcgtgggtg aacggttcgc cttctgccgt cccctgcact
    11761 tcaatgatgc gcccgtcttc ggtcatcact acgttcatgt cggtctctgc ggctgagtct
    11821 tcaacgtatt ccagatcgca aaccgcttcg ccgttcacaa ttccgacaga aactgcggct
    11881 accatccctt tcatcgggtt ggttttcagc tttccgtttt ccaccagctt ctgtagcgca
    11941 tctgccagcg ccacgcaggc acccgtaatc gacgcggtac gagtgccacc atcagcctga
    12001 agcacgtcgc agtccagcgt aatggtgaac tcacccagcg ctttcaaatc tactgccgca
    12061 cgaagagcac gggcgatcag acgctggatt tccatggtgc gtccaccctg cttacctttc
    12121 gccgcttcac gcgcgttacg ggtgtgggta gaacgtggca gcatgccgta ctctgcggtg
    12181 atccagccct ggccctgacc tttcaggaag cgcggcacgc cttcttcaat agaggcggta
    12241 cacaatactt tggtatcgcc aaattcgacc agcaccgagc cttctgcatg ttttgtatag
    12301 ttacgagtca gggtaacggg acgcacctga ttattgctac ggcctgctgg acgcatattg
    12361 aaatctccgg cttgaaacaa atgtggctgc gcattatacg gacttccggc ggttattcct
    12421 atcctgacaa ggcatcgatg gctataatcc ttccacctct ccttttataa acaggaacgt
    12481 ctatgatccg cagtatgacc gcctacgccc ggcgtgaaat caagggtgaa tgggggagcg
    12541 caacctggga aatgcgctcg gtaaaccagc gttatctgga aacttacttt cgtctgccgg
    12601 agcagttccg tagccttgaa cctgtcgttc gtgagcgtat tcgttctcgc ctgacgcgcg
    12661 gtaaagtgga atgtaccctg cgctatgagc cagatgttag cgcgcaaggt gagctgatcc
    12721 tcaacgaaaa actggctaaa cagctggtaa ctgccgcgaa ctgggtaaaa atgcagagtg
    12781 acgaagggga aatcaacccg gttgatattc tacgctggcc gggcgtgatg gcagcccagg
    12841 agcaggatct cgacgcgatt gccgctgaaa ttctcgcggc gttggatggt acgctggacg
    12901 actttatcgt cgcgcgcgaa accgaaggtc aggcgctgaa agcattgatc gagcagcgtc
    12961 tggaaggcgt caccgccgaa gtggtcaaag tccgcgccca tatgccggaa atcctgcaat
    13021 ggcagcgtga gcgtctggtc gcgaagctgg aagatgctca ggtgcaactg gaaaacaacc
    13081 gtctggagca ggagttggtt ctgctggcac aacgaattga cgttgccgaa gaactggacc
    13141 gcctcgaagc gcatgtcaaa gagacctaca acattctgaa gaaaaaagaa gcggttggtc
    13201 gtcgtctgga ctttatgatg caggagttca accgcgagtc gaacactctt gcgtcgaaat
    13261 ctatcaatgc cgaagtgacg aattctgcca tcgagctgaa agtgttgatt gagcagatgc
    13321 gcgagcagat tcagaacgtg gaataacccc cagccaactc cgaggcactc cacacaaaaa
    13381 cagcaaagcc ccattctatg gggctttttt actgtaatgc actccgatat actccaaagc
    13441 aatccagcaa aaaccgtgta cataaacgtg tacatactgg gtttatggac acatttttat
    13501 gtacacactc ccattcaata ttcaggtgga gttatgacaa aggcgaagtt aacggatatt
    13561 cagataaagg cgtggataaa ggccggggaa cgctttgatg cccgcgccga tggtaacggc
    13621 ctgtatttgt gcttccctga aaaatataac gccccattct ggcgctatcg ttaccggatg
    13681 gccgggaaac aacgtgctat gcacatcggc tcatattccg acctgtcact aagtaaagcc
    13741 cgcgccaccg ttaaagaact ggccgcccgt gttgcgctgg gccatgacgt ggccggagaa
    13801 aagcaggccc gaaaagcggc ggcactggca cggattgagg aagaaaaaaa cgcccttcgc
    13861 gtgtcagaac tggcagccga atactacgaa cggcaaatac taccccgctg gaaacatccc
    13921 gatattctcc gccgccgcat agacaaagat atcaaccctc agattggcag cctgaaagtc
    13981 gaagacgtta aaccgcgcca tattgacgca atgctacagc gcattattga ccgcggagcg
    14041 ccaacagtcg ccaatgatgt tttgcgatgg actaaacgga tattcgacta cgccatcaaa
    14101 cgccacgcac tggaaacaaa ccccgccgct gcttttgagt tatcagatgc tggtggggct
    14161 gaaaaaagcc gcgaccgatg gctaacccgt gatgaactca ttcatttttt tcaggccatg
    14221 cgcacagcta aaggattcag ccgtgagaat gaactcacca tgaaactgat actttgcctt
    14281 tgctgccgaa aaatggagtt gtgcgccgca cagtgggatg aatttgattt agatagtgct
    14341 gtatggcgac tccctgccga acgggctaaa aacggggatg ccattgatat cccactaccg
    14401 ccaccagcgg tggcatggtt gcgcgaatta cacagcattt caggcggtag ccgctgggtg
    14461 cttccggccc gcaaaatgca acagcgcatg attccacaca tacaggaaag caccctgccc
    14521 gttgcgctgg ctaaggttcg ccgggaaatg cctggtgtgc cgggttttac cgttcacgat
    14581 ttccgacgca cagccagaac tcaccttgcc gcgcttgggg tagaccctgt agtggcggaa
    14641 cgctgcctga accaccgaat taaaggcgtt gagggtatct ataaccgcca tcagtatttc
    14701 aatgaacgcc gccacgctct ggagttatgg gccgacctgc tggtggctct ggaacagggg
    14761 gatgaataca acgtaacccc catcacaaaa gcgattgcaa aataaaaaca taccggggat
    14821 agcgcgacgg cgcgaaaagc gggaaacctt accgcctgcc ccggtttatt tttaaggtag
    14881 cgtcaaaggt aacgtaaacc atgtatgaaa acacataccc tattgataca gccgccagca
    14941 tactggggtg tactcaaaaa aaaatactga ctgaatgggc taaagggaca ataaaaatcg
    15001 cacttgattt cggcccggaa agttccataa accgccaaag ctacacggat tacgacaagt
    15061 ggggatatcc ggccaagccg tggaacattg ccagtatttc agcgccagca acaaacgatt
    15121 atttcccacc tgaattacta caaccggaag aaggggagaa actcaggctt ttccccaatg
    15181 acaacgaacc tgaatcaata ttgatttgga gaaatgttta ccatcaatac tcacaatccg
    15241 ggttctactt tgacagcaac catagtgacg cgcaggtaca tgacgggaaa cttatggttg
    15301 tatcggatgt tctcctgtat ggcttatgga ctgtacctta cgccgagttc tcgcgcgttc
    15361 acgcacagga cacattcaca ttaaaaccgt acaccgatac cccttcactg tccaaagtta
    15421 cagcaacctt tacaccacgt ggcggctcac gccggataaa cgccccggaa aaaaagaatt
    15481 tgcgcattac cgattcagaa atgatggtca tgcaaaaaat acttgcacaa aggcccggct
    15541 accagcgcac agaacaaccg acagatgaca ggaatcagga aaagcatgat atacacggcg
    15601 gtgtggagcg attcgcgctg gaacgtgaaa aggttctcgc ggctgccctg tacgtggcgt
    15661 atcacttccc taaagatgtt ggcaagacac ttaaatcaca tgccgaagcc atagagaaac
    15721 acggttatct gttctgggat ggcaatccca ccccaacacc tgacgcggga cgaatagcaa
    15781 aaatattatc tgatgccaga cgcttaccca atgaatggaa aattttgggc ggaaacgcaa
    15841 aagcaaaaag ataatttttt aatgaaattg aaagcataaa agcgtttttt taatacatat
    15901 gccagcattt taaagaaacc gaaaacacca ataaaaatgg cgactaaatt tagtcgcctt
    15961 tactttttct tgccttgcgt atagcctcca ttcagcacca aaacactgta agaggcttta
    16021 atcatggata ccttacccaa atccggctat atgcgcccgg cacaggtgat aaaccatttt
    16081 cagattagcg aatcgacgct atggaactgg gttaaaaaag gtaaattccc cagcccggat
    16141 aagctgggcg atggcgtaag ccgctttgat gtttccgcta tccgccagtg gctcgctgac
    16201 cgcaaaggca cgaccagcac gcgcaattaa ccccgtcaga gaattttaat catgaaaatg
    16261 ccagaaaggc ccgcaacagg gccggggcgt tctcacgccc aaaacaacca ccacacgccc
    16321 cacagaatgg gcggcaaccg caaatattgc ttgcctgcca tcgctggtgg gcgttatatt
    16381 tccccggctg gtgtaaatcc accagccggg cgtggaaacc cgtttaatga gcaaaaggcg
    16441 acacagacgc gcccagcgtc tttttttgtg tcgtgccatc gcgcaaccat gaacagcggc
    16501 aaggcccgca ctgtatctat ggtggcgctg gtggggcagc cgaaaggctg gccggtttcc
    16561 ttttgcgccg gtatttccac ccccgccagc gtcaccgccc cttatgagcg tggaaactca
    16621 ggcggtgact ccttaaattt gcaaaaggag gctgccagaa tggctacgac cccaaccccg
    16681 ttacaccctg aatttacctt tcactttctg gccgttcgcc gttccgatct gaacgaccgc
    16741 ccacaccgcg aatccgtcaa cgcacccgac gaaacaaccg cccgccgtat gctggcgcgt
    16801 gattttgtgt tgctttttgc tggccgcgtt ccggctcagg gggtgagcca tgcttaaaaa
    16861 catgttgatc gcctcttctt tcgggctgta tgatgccgga gtcacccaca tggtgaccgg
    16921 gtttagcagc ctgaataaca tagcggacag ccgcttacat tccgatatgc ggtttttttg
    16981 tgtccgtaaa cctacccata cccgcattat ggcggggcgt gacgggggag cctttgcgct
    17041 cgctggtttc tatgttgcca gtctgctaac cccgttacgt ctcgccaccc cgtttagcag
    17101 cgtagtagcg agactcctta cctcaaacat aggagccttt cacatggctg tattcgcacg
    17161 cccttacttt gtctggcgct ttatgcagtg ccaggaaaaa caaacctcgc tctttaccgt
    17221 cacggcggca acagaacgcg aagcacgcgc gcagctaccg cacgcgcata tgatttttgt
    17281 cgcccgtatt cgtcaggggg cgcgccatgc gtgattgtct gcgtgagtca atgaaagccg
    17341 ccatgagcag tatgccggac gaagaaagcc actggattct gcgcgttgat gccgactggc
    17401 acagagtgaa tttactggcg gggattgcct ttgttggtaa ggcactggag ggaagccagt
    17461 tacgcgaaaa ccccatcacc tattccagag acgagatctg ccaattagcc ggatttctac
    17521 agacagcccc tgccctcatt ggctgtatgg ccgaactgat ggagtgttac gaccagcagg
    17581 ccggggaggt aagccatgtg tgatatgtat aacacccgga tacctgaact actggttgcg
    17641 gccatcaaaa acgcagacgc acaggaagcc cgcgccctgt ttgatgatgc ggattactgc
    17701 gcccgcaaac tgcttgaagg gctgattagc acaggccgcc tgctttccgg catgggcgac
    17761 aatctcgacc ccagcatggg cgaactacgc agcctgggcg attccatcgc tgttacggct
    17821 gaacttgtgg ccggattcag tgaggttgtg gaggcgtata actggcgctg ccgcaccggg
    17881 gaaatcaggg agggcgggca acatgcgtga catagccacg attaacgcaa tccgcagcgt
    17941 tgccgcagtg gcggcacaac atgcggaaat ccttgttcct gaatggctgc ccgaaggtaa
    18001 acggcaggcc actgaatggg tgtgccgtaa ccccacacgt gcagaccgtc acgcgggcag
    18061 ttttttcgtc tcgctggtgg acggatgctg gcacgacttc gccaccgggg acagcggcgg
    18121 cgaccttgtg gcgctggggg cttatttgtg gaatgtgcgt cagactgatg ccgcccgtat
    18181 tgtggcggac aggctggggc tgtgcctgcc cgcactgggc aaacctgatg taatgacccg
    18241 cgaacaacgg gaggcgcaac gggtgcgggt acaggccgca gaacaggagg cgggcagact
    18301 ccgccagcgg gagcagcaac aacgccacca gcgccagaaa ctgaccgcct gccgggcctt
    18361 tgagttgctt ggccgggcgg aggtggcaac cccttctcat ccctatctca tgaaaaaacg
    18421 gctacagccc ggcaatctgt atcagtcggg taatgatttg cttgtcccgc tgtacaacgt
    18481 atgcggcgaa gtggtgaacg tgcagattat cagcccggac ggtcgaaaaa tgtttctccg
    18541 tgatggtcag gtgcaggggg ctttccatgt tatggggagc tttgacctga tggccccgga
    18601 agcgccggaa catgacgttt acgtgtgtga ggggtgggcc accggggccg cccttctcca
    18661 gtactggaac gtcatggccg tggtgtgtgc catgaatgcg ggaaacctta aacatgtggc
    18721 tatggcgctg cgtgaacgat acggctcgcg catatctctc gttatcgccg gggatgatga
    18781 ccgggcatca aaaggcaacc ccggttccag agcagccaac gaagccgcat tgctggccgg
    18841 atgttacgtc acattcccgg aatggccgcc cggtgcatca ccagaccttt ccgactttaa
    18901 cgatttgatg ctatgggaaa tggagcatga cccggacaac cactaaaacc ccggtcacat
    18961 ccgggaagcg aaaaccacca cgcaagaaac accccaaagt cagcccggca cgtccgggct
    19021 acgccactta ttcgcagcat gtacaggtgg acggcggcca gatactgaaa cccggccttt
    19081 actggcacgg cgtgcgcgac tcaaccaaca cggacacgtt tatctgttcc ccgctggagg
    19141 ttatcgccat gaccagcgac agcaaagacg aaaactacgg gcgattactc cgttttctca
    19201 cgccgtcagg ccagtggcgg gaatgggccg caccgatgga aatgctcgcc ggagacggcg
    19261 gagaactccg acaggagctg ctgcgcatgg gcgttaccat tccctacaaa caacggcagg
    19321 cactgaccga atacatcatg gagtcagcac caaaacgacg ggcgctcgcg gctacggcca
    19381 ccgggtggca ctccccgacc ctgtttgtca tgccgggcgg ggttatcggt gatggtgatg
    19441 tggtgtttca gtcaacagaa gcaggctcac aggagtacgc caccgcagga acgccggagg
    19501 gctggcgcga caacgtggcc gcattatgcc ggggaaaccc ggtagccatg ctggcagtgt
    19561 gtgcatcact cgccgggccg ttgctgtggc tgcttgatgt ggacagcggc ggatttcatt
    19621 tcatgggcga cagttccagc gggaaaagcg tgggggcgct ggtggcctgt agcgtatggg
    19681 gagaaccccg caactttaaa cgcaactgga acgccaccag caccgggctt gaagggctgg
    19741 cgaccatgcg gaacgatacc gcgctgatac tggatgaaat tggcgaagcc ccggccagag
    19801 atattggcgg gctgatttat cagcttggca acggaacagg ccgccagcgt ggcaaggtgt
    19861 ccggcatggc ccgcccggtc aacacatggc gcaccatgat tatttccaca ggggaaatga
    19921 ccgtgggtaa acacatggaa agcggtggtc agcgcatcaa ggccgggcag gaaatgcggt
    19981 tactggatat tccggcacag cggctttacg gggcgtttga ctgcctccac ggaatggcac
    20041 taccccacaa ccccgacaac gacacagaac gccgtggagc aggccggaaa tttgccgaaa
    20101 cgctgcgcgg taatgcggcc acccattacg gccacgccgg gccggagttt gtgcgctggc
    20161 tgatttcccg caatgaaagt gacaccacca gcaccgacag caacggccag acgctggcgg
    20221 atatgtacgc catactgcgc gaacaattcc ccgtaagcac cgggcaggag gcgcgcgccg
    20281 ccgcgcgttt cacactgtgc gcactggccg gtgaaatggc gataactgcc gggattctgc
    20341 cgtggaaaca gggcgaagca ctggaggcgg tgaaagcgat gtttgctgcg tggtcggact
    20401 atcgtggccg ggggcaatcg gaggacgtga aaatactccg ggccgtcgcc gggtttatcg
    20461 cccgccatgc ctcacgcttt gaagggggca acacatacag cgaaccagta agcaaccgcg
    20521 ccggatggtg gcgaccgggg agcaacggcg accgggttta ctggttttta ccggaagcac
    20581 tatgtgaggc ggccagcggc tacgacctga aacgcatagc ggccagtctt gatgatgctg
    20641 gcgcgattgt tgaccgcgac acggacggga aacgccgata cacaaaacgc gtttccgtta
    20701 acggggaaaa acaccgcgtt tacgccatcg accagaatgc attgttattc gcaattgatg
    20761 aataattaac aaaaattgcg tttttaaaaa ctgaaaaagt g
//
LOCUS       DAZKGF010000087        20778 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZKGF010000087 DAZKGF010000000
VERSION     DAZKGF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.087"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1351
                     /gene="ygcU"
                     /locus_tag="ACHOQV_002941"
     CDS             <1..1351
                     /gene="ygcU"
                     /locus_tag="ACHOQV_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFP9631282.1"
                     /translation="FRKFPDIHGIYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVP
                     RTGASATEGGLETVVENSVVLDGSAMNQIINIDIENMQVTAQCGVPLEVLENTLREKG
                     YTTGHSPQSKPLAQMGGLVATRSIGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPR
                     RAAGPDIRHIIIGNEGALCYITEVTVKIFKFTPENNLFYGYVLEDMKTGFNILREIMV
                     EGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQC
                     QRVDSKLIETWFNHLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINR
                     IRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICE
                     ETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            1444..2781
                     /gene="ygcS"
                     /locus_tag="ACHOQV_002942"
     CDS             1444..2781
                     /gene="ygcS"
                     /locus_tag="ACHOQV_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9631283.1"
                     /translation="MNTSPVRMDDLPLNRFHCRIAALTFGAHLTDGYVLGVIGYAIIQ
                     LTPAMQLTPFMAGMIGGSALLGLFIGSLVLGWISDHIGRQKIFTFSFLLITLASFLQF
                     FATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFSPRRHRGVLLGAFSVVWTVGYVLAS
                     IAGHHFISESPEAWRWLLASAALPALLITLLRWGTPESPRWLLRQGRFAEAHAIVHRY
                     FGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFNSVFFVCLVIPWFVIYTWLPTIAQT
                     IGLEDALTASLMLNALLIVGALLGLVLTHLLAHRRFLLGSFLLLAATLVVMACLPSGS
                     SLTLLLFVLFSTTISAVSNLVGILPAESFPTDIRSLGVGFATAMSRLGAAVSTGLLPW
                     VLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKALPLVAAGNVGGANEHSVSV"
     gene            2759..3538
                     /gene="ygcR"
                     /locus_tag="ACHOQV_002943"
     CDS             2759..3538
                     /gene="ygcR"
                     /locus_tag="ACHOQV_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFP9631284.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNIGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEDAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEITRETVAAEAPA
                     IQCLQLARPEQRRRAALIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3535..4395
                     /gene="ygcQ"
                     /locus_tag="ACHOQV_002944"
     CDS             3535..4395
                     /gene="ygcQ"
                     /locus_tag="ACHOQV_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP9631285.1"
                     /translation="MNIAIVTINQEHAAMAGWLAAQDFSGSTLTHWQIEPQPMVAEQV
                     LDALVEQWQRTPAEVVLFPPGAFGDELSIRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAEINTMLPSGMLQLNIVPGALPDWLISIENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLVEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKAVLEA
                     LVTTIHADCQ"
     gene            complement(4542..5117)
                     /gene="ygcP"
                     /locus_tag="ACHOQV_002945"
     CDS             complement(4542..5117)
                     /gene="ygcP"
                     /locus_tag="ACHOQV_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFP9631286.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(5134..5394)
                     /gene="ygcO"
                     /locus_tag="ACHOQV_002946"
     CDS             complement(5134..5394)
                     /gene="ygcO"
                     /locus_tag="ACHOQV_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP9631287.1"
                     /translation="MSVTRNLWRVADAPHIVPADSVERQTVQRLINACPAGLFSLTPE
                     GDLRVDYRGCLECGTCRLLCDEKTLQQWRYPPSGFGITYRFG"
     gene            complement(5385..6656)
                     /gene="ygcN"
                     /locus_tag="ACHOQV_002947"
     CDS             complement(5385..6656)
                     /gene="ygcN"
                     /locus_tag="ACHOQV_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9631288.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLLGDALRSCVNTGISVRGMDMALTGTQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6734..7096)
                     /gene="queD"
                     /locus_tag="ACHOQV_002948"
     CDS             complement(6734..7096)
                     /gene="queD"
                     /locus_tag="ACHOQV_002948"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFP9631289.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHYYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            7415..9214
                     /gene="cysJ"
                     /locus_tag="ACHOQV_002949"
     CDS             7415..9214
                     /gene="cysJ"
                     /locus_tag="ACHOQV_002949"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFP9631290.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAAIEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVDGKTLTLSEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEDGEVRVFIEHNDNFRLPAN
                     PETSVIMIGLGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            9214..10926
                     /gene="cysI"
                     /locus_tag="ACHOQV_002950"
     CDS             9214..10926
                     /gene="cysI"
                     /locus_tag="ACHOQV_002950"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFP9631291.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            11000..11734
                     /gene="cysH"
                     /locus_tag="ACHOQV_002951"
     CDS             11000..11734
                     /gene="cysH"
                     /locus_tag="ACHOQV_002951"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9631292.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNEINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            12093..14759
                     /gene="cas3"
                     /locus_tag="ACHOQV_002952"
     CDS             12093..14759
                     /gene="cas3"
                     /locus_tag="ACHOQV_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFP9631293.1"
                     /translation="MEPFKYICHYWGKSSKSLTKGNDIHLLIYHCLDVAAVADCWWDQ
                     SVVLQNAFCRNEMLSKQKVKAWLLFFIALHDIGKFDIRFQYKSAESWLKLNPATPSLN
                     GPSTQMCRKFNHGAAGLYWFNQDSLSEQSPGDFFSFFDAAPHPYESWFPWVEAVTGHH
                     GFILHSQDQDKSRWEMPASLASYAAQDKQAREEWISVLEALFLTPAGLSINDIPPDCS
                     SLLAGFCSLADWLGSWTTTNTFLFKEDAPSNINALRTYFQDRQQDASRVLELSGLVSN
                     KRCYEGVHALLDNGYQPRQLQVLVDALPVAPGLTVIEAPTGSGKTETALAYAWKLIDQ
                     QLADSVIFALPTQATANAMLSRMEANASRLFTSPNLILAHGNSRFNHLFQSIKSRAFT
                     EQGQEEAWVQCCQWLSQSNKKVFLGQIGVCTIDQVLISVLPVKHRFIRGLGIGRSVLI
                     VDEVHAYDTYMNGLLEAVLKAQADVGGSVILLSATLPMKQKQKLLDTYGLHTDPEENN
                     SAYPLINWRGVNGAQRFDLLAHSEQLPPRFSIRPEPIYLADMLPDLRMLERMIVAANA
                     GAQVCLICNLVDVAQLCHQRLKELNNTQVDIDLFHARFTLNDRREKENRVISNFGKNG
                     KRNVGRILVATQVVEQSLDVDFDWLITQHCPADLLFQRLGRLHRHHRKYRPAGFEVPL
                     ATVLLPDGEGYGRHEHIYSNVRVMWRTQQHIEELNGASLFFPDAYRQWLDSIYDDAEM
                     DEPEWVIKGMDKFESAECEKRFKARKVLQWAEEYSLQDNDETILAVTRDGEMSLPLLP
                     YLQTSSGKQLLDGQVYEDLSHEQQYEALALNRVNVPFTWKRNFSEVVDEDGLLWLEGK
                     QNQDGWIWQGNNIVITYTRDEGMTRVIPANPK"
     gene            15174..16682
                     /gene="casA"
                     /locus_tag="ACHOQV_002953"
     CDS             15174..16682
                     /gene="casA"
                     /locus_tag="ACHOQV_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFP9631294.1"
                     /translation="MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDME
                     LATLALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQ
                     TKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGG
                     GFKSGLRGGTPITTFVCGIDLRSTVLLNVLTIPRLQKQFPNELHTENQPTWVKPVKPN
                     ESVPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGL
                     WPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQF
                     RNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTAL
                     RKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANINFSQADEVIADL
                     RDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG"
     gene            16675..17157
                     /gene="casB"
                     /locus_tag="ACHOQV_002954"
                     /gene_synonym="cse2"
     CDS             16675..17157
                     /gene="casB"
                     /locus_tag="ACHOQV_002954"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFP9631295.1"
                     /translation="MADEIDAMALYRAWQQLDNGACAQIRRVSEPDELRDIPAFYRLV
                     QSFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKTEQTTGISLGRALANSGRINERRI
                     FQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA
                     "
     gene            17170..18261
                     /gene="cas7e"
                     /locus_tag="ACHOQV_002955"
     CDS             17170..18261
                     /gene="cas7e"
                     /locus_tag="ACHOQV_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFP9631296.1"
                     /translation="MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSL
                     KHAMRKSDYYAQNIGESSLRTIHLAQLRDVLRQKLGERFDQKIIDKTLALLSGKLVDE
                     AEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDI
                     ALSGRMATSGMMSELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQE
                     FSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPA
                     DMVMVNFSDMPLSMANAFEKAVKAKDGFLQPSIQAFNQYWDRVANGYGLNGAAAQFSL
                     SDVDPITAQVKQMPTLEQLKSWVRNNGEA"
     gene            18264..18938
                     /gene="cas5e"
                     /locus_tag="ACHOQV_002956"
     CDS             18264..18938
                     /gene="cas5e"
                     /locus_tag="ACHOQV_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFP9631297.1"
                     /translation="MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACL
                     GIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHE
                     TIQTWREYLCDASFTVAIWLTPQATMVMSELEKAVLKPRYTPYLGRRSCPLTQPLFLG
                     TCQASDPQKALLNYEPVGGDIYSEESVDGHHLKFTVRDEPMITLPRQFASREWYVIKG
                     GMDVSQ"
     gene            18925..19524
                     /gene="cas6e"
                     /locus_tag="ACHOQV_002957"
     CDS             18925..19524
                     /gene="cas6e"
                     /locus_tag="ACHOQV_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFP9631298.1"
                     /translation="MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKR
                     NTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKR
                     LDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQT
                     VCFEGVITINDAPALIDLVQQGIGPAKSMGCGLLSLAPL"
     gene            19540..20457
                     /gene="cas1e"
                     /locus_tag="ACHOQV_002958"
     CDS             19540..20457
                     /gene="cas1e"
                     /locus_tag="ACHOQV_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFP9631299.1"
                     /translation="MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQ
                     AKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYVLLAKQYGVKW
                     NGRRYDPKDWEKGDTVNQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYD
                     IADIIKFDTVVPKAFEIARRIPGEPDREVRLACRDIFRSSKTLVKLIPLIEDVLAAGE
                     IQPPAPPEDAQPVAIPLPVSLGDAGHRSS"
     gene            20459..20743
                     /gene="cas2e"
                     /locus_tag="ACHOQV_002959"
     CDS             20459..20743
                     /gene="cas2e"
                     /locus_tag="ACHOQV_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFP9631300.1"
                     /translation="MSMLVVVTENVPSRLRGRLAIWLLEVRAGVYVGDVSARVREMIW
                     EQISGLAEEGNVVMAWATNTESGFEFQTFGVNRRTPVDLDGLRLVSFLPV"
BASE COUNT         5286 a         4864 c         5458 g         5170 t
ORIGIN      
        1 ttttcgcaaa tttccggata ttcatggcat ttatactttg ccgattccgg cagcggtcgt
       61 aaaactcggt tccacagaac aagtctctcg tgtactgaat tttatgaatg cacacaaaat
      121 taacggtgtg ccgcgtaccg gtgcttccgc taccgagggt ggactggaaa ccgttgtgga
      181 aaactcggtg gtgctcgacg gctctgccat gaatcaaatc attaatattg atattgagaa
      241 tatgcaggtc acggcgcaat gtggtgttcc gctggaggtg ctggaaaaca cgttgcgtga
      301 aaaaggttac accacggggc attcaccgca atcaaaaccg ctggcgcaga tgggcggcct
      361 ggtagcaacc cgcagtatcg ggcagttctc cacactctac ggcgcaatcg aagatatggt
      421 cgttggtctg gaagcagtat tggctgatgg caccgtcaca cgtattaaaa acgtaccacg
      481 ccgcgcggca ggcccggaca ttcgtcacat tatcatcggc aacgaaggtg cattgtgcta
      541 tatcactgaa gtcacagtga aaatcttcaa atttacgccg gaaaacaatc tcttctatgg
      601 ctatgtgctg gaagacatga aaaccggctt caacatcctg cgtgaaatca tggtggaagg
      661 gtatcgtccg tcgatcgctc gtttgtatga cgctgaagat ggcacccaac acttcaccca
      721 ttttgccgat ggtaaatgtg tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa
      781 ggcgacaggc gaagggattg ctgaaatcgt ggcacgttac ccgcaatgcc agcgagtgga
      841 cagcaagctg atcgaaacct ggtttaatca cctgaactgg gggccggata aagtggccgc
      901 tgaacgtgtg cagatcctca aaaccggcaa catgggcttt accaccgaag tgtccggctg
      961 ctggagctgc atccacgaaa tctacgaaag cgttattaac cgtattcgta ccgagttccc
     1021 gcatgccgac gacatcacca tgctgggcgg tcactcctct catagctatc agaacggcac
     1081 caacatgtac ttcgtctacg actacaacgt cgttgactgt aagccggaag aggaaatcga
     1141 caagtaccac aatccgctca acaaaatcat ctgcgaagaa accattcgcc tcggtggttc
     1201 gatggtgcac caccacggta tcggtaaaca tcgcgttcac tggagcaaac tggaacacgg
     1261 cagcgcgtgg gcgttgctgg aagggctgaa aaagcagttc gatcctaacg gcattatgaa
     1321 tacgggtact atctatccga ttgaaaaata atgtgtcagg cagcgttccg cgatgacggg
     1381 cgttgccttt tcggcttctc aggcgagaag ccgttcttat taccggacaa tgaaggggta
     1441 aagatgaaca cttcaccggt gcgaatggat gatttaccac ttaaccgttt tcactgccgc
     1501 attgctgcgc tcactttcgg cgcacacctg accgacggtt atgtcctcgg cgtcattggt
     1561 tacgccatta ttcagcttac acccgccatg caactgacgc cgtttatggc gggaatgatc
     1621 ggcggctcgg cgctacttgg cctgtttatc ggtagcctgg ttttggggtg gatctccgac
     1681 catattggtc ggcaaaaaat cttcaccttc agctttttgc tgattacgct cgcttcgttc
     1741 ttacaatttt ttgccaccac gccagagcat cttattggac tgcgcatttt gatcggcatt
     1801 ggtctgggag gcgattactc agtcggtcac actttgctgg ctgaattttc tccgcgccgc
     1861 catcgcggtg ttttgctggg cgcattcagc gtggtgtgga ccgtaggcta tgtgctggca
     1921 agtattgccg gacatcactt tattagcgaa agcccggagg cctggcgctg gctactggca
     1981 tcggcagctc tgcccgcatt gttgattacg ttattacgct ggggaacgcc agaatcgcca
     2041 cgctggctac tgcgccaggg gcgttttgca gaagctcacg ctatcgtgca tcgctatttt
     2101 ggtccccatg ttttactggg cgatgaagtg gcaacggcga cccataaaca catcaaaacc
     2161 ttgttctctt cgcgctactg gcggcgcaca gcgtttaaca gcgtcttctt tgtctgcctc
     2221 gtaatcccat ggtttgtgat ttatacctgg ctgccgacca tcgcccaaac tattggtctg
     2281 gaagatgcgc tgactgccag cctgatgctt aatgcgttgt taattgttgg cgcgttatta
     2341 gggttagtgt tgacccactt gttagcgcat cgcagatttt tactgggaag ttttttgctg
     2401 ctggcggcaa cgctggtcgt gatggcctgt ttgccttccg gcagttcatt aacgctgctg
     2461 ctttttgttc tcttcagcac caccatttcg gcagtcagta atctggtggg cattttgcct
     2521 gcggaaagtt ttcctactga cattcgctcg ctgggcgtcg gttttgccac cgccatgagt
     2581 cggttggggg cggcggtaag tactggcctg ctgccgtggg tgctggcgca gtggggaatg
     2641 caagccacct tattgctcct ggcggcagtg ttgttggttg gttttgttgt gacctggcta
     2701 tgggcaccag aaaccaaagc actcccgctg gtggcggcgg gaaatgtagg aggtgcgaat
     2761 gaacattctg ttagcgttta aagccgaacc ggatgccgga atgctggcgg aaaaagagtg
     2821 gcaggcggcg gctcagggta atatcggacc ggatgtttcg cttctgcgaa gtttactcgg
     2881 tgccgatgaa caggctgccg ccgcgctgct gttggcgcag agaaaaaacg gtacgccgat
     2941 gtctttaacc gcgttgagca tgggggatga acgggcgttg cactggctgc gctatctcat
     3001 ggctcttggg tttgaggacg ctgtattgct ggagacggcg gcagatctgc gctttgcgcc
     3061 ggaatttgtc gctcgccata ttgccgaatg gcagcgtcag aatccgctgg atctgatcat
     3121 taccggctgc caaagcagcg aagggcagaa tggacaaacg ccatttttgc tggcggaaat
     3181 gctgggctgg ccctgtttca cccaggtgga gcgttttact ctcgacgcgc cgtttatcac
     3241 cctcgaacag cgtactgaac atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat
     3301 tgccgtgcgt cagtgtggtg aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc
     3361 agcggggaaa gcggagatta cccgcgaaac tgttgccgcg gaagcgccag cgatacagtg
     3421 cctgcaactg gcaaggccag agcaacgacg cagagcggcg ctgattgatg ggcaaacggt
     3481 agcagaaaaa gcgcaaaagc tatggcagga ctatttacgc caaaggatgc agccatgaat
     3541 atcgcaattg tgacaataaa tcaggaacat gccgcaatgg cgggctggct ggcggcgcag
     3601 gatttcagcg gcagcacact aacgcattgg caaatagaac cacagccgat ggttgcggaa
     3661 caggtgctgg atgcgctggt ggaacagtgg caacgaacgc ctgcggaggt tgtccttttt
     3721 cctccgggcg cgtttggcga tgaactgtcg atccggctgg cctggcgttt acatggtgca
     3781 agcatctgcc aggtgacttc gttagatatt ccaaccgtaa gcgtgcgcaa atcccactgg
     3841 ggaaatgcgc taacggcgac gctgcaaaca gaaaaacgcc cgctatgtct ttccctggcg
     3901 cgtcaggcgg gagcagagat aaacaccatg ttgccctctg gtatgctgca actgaacatt
     3961 gtccccggtg cgcttcccga ctggctgatt agcatagaaa acctgaaaaa tgttacccgc
     4021 gatccattag ctgaagccag acgggtgctg gtggtggggc agggcggaga ggcagataac
     4081 caggaaatcg ctatgctggt agaaaagctg ggagccgaag tgggctacag tcgggcgcga
     4141 gtgatgaacg gtggcgttga tgctgaaaaa gtgattggta tttccggaca tctgctggcg
     4201 cctgaggtgt gcattgttgt tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc
     4261 aatagcaagt ttgtggtggc gattaatcat gacgccagcg cggcggtatt ttcacaggca
     4321 gatgttggcg tggttgatga ctggaaagcg gtgctggagg cactggtgac aaccattcac
     4381 gcagattgcc aataacgtgc tgccggatga tcctgcctac ggttctgggc tggggacttg
     4441 taagcctgat aagacgcgtc aagcgtcgta tcaggcgctg aatgtcggat gtggcgtgca
     4501 gcctacaaat cacacaaatt caactcgtta cgagtatccc gtcaaagcaa ttttttcgct
     4561 aacgtccaga ccccggtatt cgtggtggaa agcgccacaa cacccgcgtt aatcgcatta
     4621 cgcgcatctt cttcatcgca caccagcccg ccggcaatca gcggttggcg gattttctcc
     4681 gtcacccagc ccagcacttt aggcatacag cctggcagga tctcaataca atccggattc
     4741 gattgcgcaa cttgcttatc aatgttgtga aacgaaatcg aatcaacaat aaacaggcga
     4801 tgaatacaga aaaaaccttc cgctcttgcc gctttcaaca ttgaggcttt ggtactgata
     4861 atgccgtccg cctcagtcac cagtttcaaa aactgaatca ccacttcttt atttgacgcg
     4921 ccttccagca aatcaacatg aataaaagca tattttccgg catttttaat ctttttaacg
     4981 atttgactaa tggtgcagat attgccgtac aacacggaaa taaactggca ttcagagtcg
     5041 attgccagtt gcaggctggc attatcttta acggcagcaa tcaccggatt ctggcggagc
     5101 aggtgtaaga ggggcataaa ttatccttat tatttatcca aaacggtagg tgatgccgaa
     5161 tccggaaggc ggatagcgcc actgttgtag tgttttttca tcgcacagca aacggcaggt
     5221 gccacactcc aggcagccgc gataatcaac gcgtaagtca ccttccggtg tgagcgaaaa
     5281 aagaccagcc ggacaagcgt taatcaaccg ttgtaccgtc tgacgctcaa cggagtcagc
     5341 cggtacaatg tgcggcgcat cggcaacgcg ccagagatta cgggttacag acatcgcaga
     5401 ctcctgataa catcgcccgc cagatgccac aggccgtgac gacgtaaatg acgccagaat
     5461 aactggcgca gcggtggaac aggtttatca ccctgatccc ataaatcgcg ggaaatatcc
     5521 tgcattaacg caggccacgc acggtaccat ccagggcgtt gcaaaagcgc cggaacatgc
     5581 tgataacgtt gtagaacatc ccatagcagg ctgcgctcta cgttgtgatg ataaagcgca
     5641 aacagatttt gcggctcgcg gtgctggcag gcgcttatca gcgtttgtgc cgccgcctgc
     5701 gtgccagtca gcgccatatc catgccgcgc acggaaattc cggtattgac gcaactgcgc
     5761 aacgcatcgc ccagcagcag ccagccgtta ccggcgtatt gcaccggcat actgtgcaag
     5821 ccaccttctg gcaccagatg cgcaccatac tccagtgatt ccgtgttttt gataagcggg
     5881 cgcactgccg gatgcgtttt aaagcgagcc agcagctcgc ttgccggaac acgactttgc
     5941 gtaagggaag agagcgggca aacaatccct aacgagagcg tttgttgatt agtataaaga
     6001 aatgcgccgc cgggtaagtc atcacagatc ccgccgctga acagcaacgc tgcgccttcg
     6061 ttattctcca gatgaaaacg ttcttcaata gcggatgttt ccagcgacag cacttctttg
     6121 atccccaacg ccatcgcttc gccagcagga cgagtcacta acccgtgacg ttcagccagg
     6181 acgctgttgg caccttctgc cagcaccaca taacgggcgc ggagaatatc gtcaccacaa
     6241 atgacaccac acaccctgcc gttttcttca tacagcgcat ccaccgtcgc accggggatg
     6301 cattcgacac cttctttttc ggcttcggca accagccacg gatcgaaccg tgcacgtaat
     6361 acactccagg attcaccgcc gggctgtaag ctggaaaacg tcgttgcgcc atccggcgtt
     6421 aacagggaaa ggctttcgtg agtgatgcgt cgttcaagag gcgcggtcag gtgaaactga
     6481 gggaggagtt ccgcgagtgc atgggtatat aaccgcccgc cggaaagatt tttgctgccg
     6541 gggatttcag cgcgttccag taacaaaacg gataaacccg ctcgcgcgca gcgtaacgcg
     6601 caagcggtgc ctgcaatacc ggcaccaata ataataatgt cgcagtcgtc ttccatgtca
     6661 tgccccagat atccagaaca caacacccta acatagcgtt acttaaggga aattgaccgc
     6721 cgacacactc ttatcattcg ccgcgataga tgcaacctgc ggtgcaggtt tcttttacca
     6781 tcaccgcact taacagcggc acaacgggtt taacttgatc ccaaatccat tttgctaaaa
     6841 cctcgctggt cgggttttcc agacctggaa tatcattgag ataatagtga tcgaggcgct
     6901 cgtaggttgg cttaaacgcc gctttcagtt cagcgaaatc gataatccag cccgtatgcg
     6961 gatcgacttc cccggtaatt tccagtcgca ccataaagga atgcccgtgc aggcgaccac
     7021 atttatgccc ttccgggacg tgtggtaagc ggtgagcggc ttcgaaggtg aaatctttaa
     7081 ataacgtggt ggacatcata atttctctac aggacgataa aaccgccgta gagtaccgga
     7141 aaggctgttt tttcgcatta tctaaaacga ctttgttgcg caaaatcgct gatttatctt
     7201 aatgattggc taaattcatt tgtttttcat tgggttggtt aatctgtttt gttgttaaag
     7261 actattgcta aaacaggtta gtcgatttgg ttattagtta tcgctatccc gtctttaatc
     7321 cacaccgttc accccgttaa ccttacctcc tcttctgttt tatgggcgct gacagggcgc
     7381 agaaacagct ttgcttactg gaacataacg acgcatgacg acacaggtcc caccttccgc
     7441 gttgcttccg ttgaacccgg agcaactggc acgccttcag gcggccacga ccgatttaac
     7501 tcctacccag cttgcctggg tttctggcta tttctggggc gtactcaatc agcagcctgc
     7561 tgcgcttgca gcgacgccag cgccagccgc agaaatgccg ggtataacta ttatctccgc
     7621 ctcgcaaacc ggcaatgcgc gccgggttgc tgaagcatta cgcgatgatt tattagcagc
     7681 aaaactgaac gttaagctgg tgaacgcggg cgactataaa ttcaaacaaa tcgccagcga
     7741 aaaactgctc atcgtagtga cgtcaacgca aggggaaggg gagccgccgg aagaagccgt
     7801 cgcgctgcat aagttcctgt tctccaaaaa agcgccgaag ctggaaaata ccgcttttgc
     7861 cgtgtttagc ctcggcgata gttcttatga atttttctgc cagtccggga aagattttga
     7921 cagtaagctg gcggaactgg gcggtgaacg cctgctcgac cgtgtcgatg ccgacgttga
     7981 ataccaggct gctgccatcg agtggcgcgc ccgcgtggtt gatgcgctta aatcgcgtgc
     8041 gcctgtcgcg gcaccttcgc aatccgtcgc tactggcgcg gtaaatgaaa tccacaccag
     8101 cccgtacagc aaagacgcgc cgctggtggc gagcctctct gttaaccaga aaattaccgg
     8161 gcgtaactct gaaaaagacg ttcgccatat cgaaattgac ttaggtgact ctggcctgcg
     8221 ttaccagccg ggtgacgcgc tgggcgtctg gtatcagaac gatccggcac tggtgaaaga
     8281 acttgtcgaa ctgctgtggc tgaaaggcga tgaacctgtc accgttgacg gcaaaacgct
     8341 gaccctgagc gaagcgctac agtggcactt tgaactgacc gtcaacaccg cgaatattgt
     8401 tgagaactac gccacgctta cccgcagcga aacgctgctg ccgctggtgg gcgataaagc
     8461 gaagttacag cattacgccg cgaccacgcc gattgttgac atggtgcgtt tctctccggc
     8521 gcaactggat gccgaagcgc tgattaacct gctgcgcccg ctgaccccgc gcctgtattc
     8581 catcgcctcc tcgcaggcgg aagtcgagaa cgaagtgcac gttaccgttg gtgtggtgcg
     8641 ttacgacgtg gaaggccgcg cccgtgccgg tggtgcctcc agcttcctcg cagatcgtgt
     8701 ggaagaagat ggtgaagtcc gcgtctttat cgaacataac gacaacttcc gcctgccagc
     8761 caatccggaa acgtcggtga ttatgattgg cctaggcacc ggcatcgcgc cgttccgcgc
     8821 ctttatgcag caacgcgccg ccgacgaggc gccggggaaa aactggctgt tctttggcaa
     8881 cccgcacttt acggaagatt tcctctacca ggtggaatgg cagcgctacg tcaaagaggg
     8941 cgtgctgacg cgtatcgatc ttgcctggtc gcgcgatcaa aaagaaaaaa tttacgtaca
     9001 agacaaactg cgcgaacagg gcgcggagct gtggcgctgg atcaatgacg gtgcccacat
     9061 ttatgtctgc ggcgacgcta atcgcatggc gaaagacgtt gagcaggcac ttctggaagt
     9121 gattgccgaa tttggtggca tggacaccga agcggcggat gaatttttaa gtgagctgcg
     9181 cgtagagcgc cgttatcagc gagatgtcta ctaatgagcg aaaaacatcc agggccttta
     9241 gtggtcgaag gaaaactgac agacgccgag cgcatgaagc ttgaaagcaa ctacctgcgc
     9301 ggcaccattg cggaagattt aaacgacggt ctgaccggcg gctttaaggg cgacaacttc
     9361 ctgctgatcc gcttccacgg catgtatcag caggatgacc gcgacatccg cgccgaacgt
     9421 gctgaacaga agctggagcc gcgccacgcg atgctgttgc gctgccgtct gccgggtggc
     9481 gtgatcacca ccaaacagtg gcaggcgata gacaaatttg ccggtgaaaa caccatctat
     9541 ggcagcattc gcctgaccaa ccgccagacg tttcagttcc acggcattct gaaaaagaac
     9601 gtcaaaccgg tgcaccagat gctgcactcg gtcggccttg atgcgctggc gactgctaac
     9661 gacatgaacc gtaacgtact ctgcacctcg aacccttacg agtcgcaact gcacgcggaa
     9721 gcatatgaat gggcgaagaa aatctctgaa catctgctgc ctcgtacccg cgcgtatgcg
     9781 gagatctggc tcgatcagga aaaagtcgcc actactgatg aagagccgat ccttggtcag
     9841 acctacctgc cgcgtaagtt caaaaccacg gtagtgatcc cgccgcagaa cgatatcgat
     9901 ctgcacgcca acgacatgaa cttcgtggca atcgccgaaa acggcaagct ggtgggcttt
     9961 aacctgttgg tgggcggtgg gctttccatc gaacacggca acaagaaaac ctacgcccgc
    10021 accgcgagtg agtttggcta tctgccgctg gagcatacgt tggcagtggc ggaagccgtc
    10081 gtgacaactc agcgtgactg gggtaaccga accgatcgta aaaatgccaa aaccaaatac
    10141 acgctggagc gcgtgggggt tgagacgttt aaagcggaag tggagcgtcg cgcggggatc
    10201 aaatttgaac cgatccgtcc gtatgagttt accggacgcg gcgatcgtat tggctgggtt
    10261 aagggcattg atgataactg gcatctgacg ctgtttatcg aaaatggtcg catccttgat
    10321 tatcctgggc gtccgctgaa aaccggcctg ctggagatcg cgaagatcca caaaggcgat
    10381 ttccgcatta cggcgaacca gaatctgatc atcgccggtg tgccggaaag cgagaaagcg
    10441 aagatcgaga agatcgccaa agagagcggg ttaatgaatg ccgtcacgcc gcagcgtgaa
    10501 aactcaatgg cctgcgtgtc attcccgact tgcccgctgg cgatggccga agccgagcgt
    10561 ttcctgccgt cttttatcga caacatcgat aatttaatgg cgaaacatgg tgtcagcgat
    10621 gagcatatcg tgatgcgtgt aacaggctgc ccgaacggtt gtggtcgcgc gatgctggcg
    10681 gaagtgggcc tggtgggtaa agcgccaggt cgctacaact tgcatcttgg cggcaaccgc
    10741 attgggacac gtatcccacg gatgtataaa gaaaacatca ccgagccgga aatcctggcg
    10801 tcgcttgatg aactgatagg gcgctgggcg aaagagcgcg aagcgggtga aggcttcggc
    10861 gactttacgg tgcgtgcggg catcattcgc ccggtgctcg atccggcgcg cgatttgtgg
    10921 gattaaccat tagcccggtc ttgtaggcct gataagacgc gtgagcgtcg catcaggcaa
    10981 ggcaaacagt gaggaatcta tgtccaaact cgatctaaac gccctgaacg aactgccgaa
    11041 ggtagatcgc attctggcgc tggcggaaac taacgccgaa ctggaaaaac tggacgctga
    11101 aggccgcgtt gcctgggcgc tggataatct gcccggtgaa tatgtacttt cttccagctt
    11161 tggcattcag gcggcggtga gcctgcatct ggtgaatcag attcgcccgg atattccggt
    11221 gatcctcacc gataccggct atctgttccc agaaacctac cgctttattg acgagttaac
    11281 ggacaaactc aagctcaacc tgaaagtgta ccgcgctacc gaaagcgccg cctggcagga
    11341 agcgcgctac ggtaaactgt gggagcaggg tgttgagggg attgagaagt acaacgagat
    11401 caacaaagtc gaaccgatga accgggcgct gaaagaactg aatgcgcaaa cctggtttgc
    11461 tggcctgcgc cgtgaacaat ccggcagccg cgccaattta ccggtgctgg caattcagcg
    11521 tggcgtattt aaagtgctgc cgattatcga ctgggataac cgaactattt atcagtacct
    11581 gcaaaaacat ggcctgaaat atcacccatt atgggacgaa ggatatttat cggtcggtga
    11641 tacccataca acccgtaaat gggaacccgg catggcggaa gaagaaacgc gtttctttgg
    11701 cttaaaaagg gaatgtgggt tacacgaagg gtaaatattg ccggataaat taaacgcctc
    11761 tgtcagaaat gatgggggcg tttttatttt gcgagcagca ttccggcatt taaaggtgaa
    11821 catcgcaggg agaattaaca ggtatagaat ggagttgggt gcttgaggct gtctggcgtt
    11881 aagcattcgc gaggttccag atggacaaaa gccccaggcg atatttctat caacctgagg
    11941 ccagcgttcg aacctaagca attcgaatgt tagtctcttc tttgcaggga ggcaagacat
    12001 gtgtatatca ctgtaattcg atatttatga gcagcatcga aaaatagtcc actgatatca
    12061 tcgataatac taaaaaaaca gggaggctat taatggaacc ttttaaatat atatgtcatt
    12121 actgggggaa atcctcaaaa agcctgacga aaggaaatga tattcatctg ttgatttatc
    12181 attgcctgga tgttgcagct gttgcagatt gctggtggga tcaatcagtc gtactacaaa
    12241 atgctttttg ccgaaatgaa atgctatcaa aacagaaggt gaaagcctgg ctgttatttt
    12301 tcattgccct tcatgatatc ggaaagtttg atatacgatt ccaatataaa tcagcagaaa
    12361 gttggctgaa attaaatcct gcaacgccat cacttaatgg tccatcaaca caaatgtgcc
    12421 gtaaatttaa tcatggtgca gccggtctgt attggtttaa ccaggattca ctttcagagc
    12481 aatctcccgg ggattttttc agtttttttg atgccgctcc tcatccttat gagtcctggt
    12541 ttccatgggt agaggctgtt acaggacatc atggttttat attacattcc caggatcaag
    12601 ataagtcgcg ttgggaaatg ccagcttctc tggcatctta tgctgcgcaa gataaacagg
    12661 ctcgtgagga gtggatatct gtactcgaag cattattttt aacgccagcc gggttatcta
    12721 taaacgatat accacctgat tgttcatcac tgttagcagg tttttgctcg cttgctgact
    12781 ggttaggctc ctggactaca acgaatacct ttctgtttaa agaggatgca ccttccaaca
    12841 taaatgctct aagaacgtat ttccaggacc gacagcagga tgcgagccgg gtattggagt
    12901 tgagcggcct tgtatcaaat aagcgatgtt atgaaggtgt tcatgctcta ctggacaatg
    12961 gctatcaacc cagacaatta caagtgttag ttgatgctct tccagtagcg cccgggctga
    13021 cggtaataga ggcacctacg ggttcaggta aaactgaaac tgcgctggcc tatgcctgga
    13081 aacttattga ccaacaactt gcggatagtg ttatttttgc cctccctaca caagctactg
    13141 caaatgcaat gctcagcaga atggaagcga acgcgagccg tttatttacc tccccaaatc
    13201 ttattcttgc tcatggtaat tcacgtttta accacctttt tcaatcaata aaatcacgcg
    13261 catttactga gcaggggcaa gaagaagcat gggttcagtg ttgtcagtgg ttgtcacaaa
    13321 gcaataagaa agtgtttctt gggcaaatcg gcgtttgcac gattgatcag gtgttgatat
    13381 cggtattgcc agttaaacac cgctttatcc gtggtttggg aattggtcga agtgttttaa
    13441 ttgttgatga agttcatgct tacgacacgt atatgaacgg cttgctggag gctgtgctca
    13501 aggctcaggc tgatgtgggg gggagtgtta ttcttctttc cgcaactcta ccaatgaaac
    13561 aaaaacagaa acttctggat acttatggtc tgcatacaga tccagaggaa aataactccg
    13621 catatccact cattaactgg cgaggtgtga atggtgcaca acgttttgat ctgttagctc
    13681 attcagaaca actcccgccc cgcttttcga ttcggccaga acctatttat ttagctgaca
    13741 tgttacctga ccttaggatg ttagagcgaa tgatcgtagc ggcaaatgcg ggtgcacaag
    13801 tctgtcttat ctgcaatttg gttgatgttg cacagctatg ccaccaacgg ttaaaggaac
    13861 taaataacac gcaagtagat atagatttgt ttcatgcgcg ctttacgctg aacgatcgtc
    13921 gtgaaaaaga gaatcgagtt attagcaatt tcggcaaaaa tgggaagcga aatgttggac
    13981 ggatacttgt cgccacccag gtcgtggaac aatcactcga cgttgatttt gattggttaa
    14041 ttactcagca ttgccccgca gatttacttt tccaacgatt gggccgttta catcgccatc
    14101 atcgcaaata tcgtcccgct ggttttgagg ttcctcttgc caccgttttg ctgcctgatg
    14161 gcgaaggtta cggacgacat gagcatattt atagcaacgt tagagtcatg tggcggacgc
    14221 agcaacatat tgaggaactt aatggagcat ccttattttt ccccgatgcc taccggcaat
    14281 ggctggatag catttacgat gatgctgaaa tggatgagcc agaatgggtc atcaaaggca
    14341 tggataagtt tgaaagcgct gagtgtgaaa agaggttcaa ggctcgcaag gttctgcagt
    14401 gggctgaaga atatagcttg caggataacg atgaaacaat tcttgcggta acgagggatg
    14461 gggaaatgag cctgccatta ttgccttatt tacaaacgtc ttcaggtaaa caactgctcg
    14521 atggccaggt ctacgaggac ctaagtcatg aacagcagta tgaggcgtta gcacttaatc
    14581 gcgtcaatgt acccttcacc tggaaacgta atttttctga agtagtagat gaagatgggt
    14641 tactttggct ggaagggaaa cagaatcagg atggatggat ctggcagggt aataatattg
    14701 ttattaccta cactcgggat gaagggatga ccagagtcat ccctgcaaat cccaaataac
    14761 ttcgggaatg attggtatca atgacgataa caagaccaat aacggtttat ccctacttaa
    14821 gtagggaaag tgcacaatgt acatcttctt ttaatttccc ggtatgagat tttatattca
    14881 cagtatgaat attttatgta ataaaattta tggtaattat tataactaaa aatttctttg
    14941 ataataaaac gaataacttg cagatttgaa atgcatgcat tattgtcttt aaacaattca
    15001 acacatctta atatatgtat aggttaattg tattaaacca atgaatatat ttttgcagtg
    15061 aatgtgatta ttgaattaat tacgccgtat tttttctttg tttttaccga taacggaagt
    15121 gtgccgacgc atagaaatgc aggagaaatg tcggagcata tgaaggagaa caaatgaatt
    15181 tgcttattga taactggatc cctgtacgcc cgcgaaacgg ggggaaagtc caaatcataa
    15241 atctgcaatc gctatactgc agtagagatc agtggcgatt aagtttgccc cgtgacgata
    15301 tggaactggc cactttagca ctgctggttt gcattgggca aattatcgcc ccggcaaaag
    15361 atgacgttga atttcgacat cgcataatga atccgctcac tgaagatgag tttcaacaac
    15421 tcatcgcgcc gtggatagat atgttctacc ttaatcacgc agaacatccc tttatgcaga
    15481 ccaaaggtgt caaagcaaat gatgtgactc caatggaaaa actgttggct ggggtaagcg
    15541 gcgcgacgaa ttgtgcattt gtcaatcaac cggggcaggg tgaagcatta tgtggtggat
    15601 gcactgcgat tgcgttattc aaccaggcga atcaggcacc tggttttggt ggtggcttta
    15661 aaagcggttt acgtggagga acacctataa ccaccttcgt atgtgggatc gatcttcgct
    15721 cgacggtatt actcaatgtc ctcacaatac ctcgtcttca aaaacaattt cccaatgaat
    15781 tacatacgga aaaccaacct acctgggtta agcctgtcaa gccgaatgaa tcggtgcctg
    15841 cttcttcaat tgggtttgtc cgtggtttat tctggcaacc agcgcatatt gaactatgcg
    15901 atcccattgg gattggtaaa tgttcttgct gtggtcagga aagcaatttg cgttataccg
    15961 gttttcttaa ggaaaaattt acctttacag ttaatgggct atggccccat ccgcattccc
    16021 cttgtctggt aacagtcaag aaaggggagg ttgaggaaaa atttcttgct ttcaccacct
    16081 ccgcaccatc atggacacaa atcagccgag ttgtggtaga taagattatt caaaatgaaa
    16141 atggaaatcg cgtggcggcg gttgtgaatc aattcagaaa tattgcgccg caaagtcctc
    16201 ttgaattgat tatgggggga tatcgtaata atcaagcatc tattcttgaa cggcgtcatg
    16261 atgtgttgat gtttaatcag gggtggcaac aatacggcaa tgtgataaac gaaatagtga
    16321 ctgttggttt gggatataaa acagccttac gcaaggcgtt atataccttt gcagaagggt
    16381 ttaaaaataa agacttcaaa ggggccggag tttctgttca tgagactgca gaaaggcatt
    16441 tctatcgaca gagtgaatta ttaattcccg atgtattggc gaatattaat ttttcccaag
    16501 ctgatgaggt aatagctgat ttacgagaca aacttcatca attgtgtgaa atgctattta
    16561 atcaatctgt agctccctat gcacatcatc ctaaattaat aagcacatta gcgcttgccc
    16621 gcgccacgct atacaaacat ttacgggagt taaaacctca aggagggcca tcaaatggct
    16681 gatgaaattg atgcaatggc tttatatcga gcctggcaac aactggataa tggagcatgt
    16741 gcgcaaatta gacgtgtttc agaacctgat gaattacgcg atatccctgc gttttatagg
    16801 ctggtgcaat cttttggttg ggaaaaccca cgtcaccagc aggctctttt gcgcatggtg
    16861 ttttgcctga gcgcaggaaa gaatgtcatc cgacatcagg acaaaaaaac ggagcaaaca
    16921 acaggtatct cgttgggaag agctttagcc aatagtggaa gaattaacga gcgccgtatc
    16981 tttcaattaa ttcgggctga cagaacagcc gatatggtcc agttacgtcg attacttact
    17041 cacgccgaac ccgtacttga ctggccatta atggccagga tgttgacctg gtggggaaag
    17101 cgcgaacgcc agcaacttct ggaagatttt gtattgacca caaacaaaaa tgcgtaagga
    17161 aacctttcta tgtctaactt tatcaatatt catgttctga tctctcacag cccttcatgt
    17221 ctgaaccgcg acgatatgaa catgcagaaa gacgctattt tcggcgggaa aagacgagta
    17281 agaatttcaa gtcaaagcct taaacatgcg atgcgtaaaa gtgattatta cgcacaaaat
    17341 attggtgaat ccagtctcag aacaattcat cttgcacaat tacgtgatgt tcttcggcaa
    17401 aaacttggtg aacgttttga ccaaaaaatc atcgataaga cattagcgct gctctccggt
    17461 aaattagttg atgaagccga aaagatttct gccgatgcgg ttactccctg ggttgtggga
    17521 gaaatagcct ggttctgtga gcaggttgca aaagcagagg ctgataatct ggatgataaa
    17581 aagctgctca aagttcttaa ggaagatatt gccgccatac gtgtgaattt acagcagggt
    17641 gttgatattg cgcttagtgg aagaatggca accagcggca tgatgagtga gttgggaaaa
    17701 gttgatggtg caatgtccat tgcgcatgcg atcactactc atcaggttga ttctgatatt
    17761 gactggttca ccgctgtaga tgatttacag gaacaaggtt ctgcacatct gggaactcag
    17821 gaattttcat cgggtgtttt ttatcgttat gccaacatta acctcgctca acttcaggaa
    17881 aatttaggtg gtgcctccag ggagcaggct ctggaaattg caacccatgt tgttcatatg
    17941 ctggcaacag aggtccctgg agcaaaacag cgtacttatg ccgcttttaa ccctgcggat
    18001 atggtaatgg ttaatttctc cgatatgcca ctttctatgg caaatgcttt tgaaaaagcg
    18061 gttaaagcga aagatggctt tttgcaaccg tctatacagg cgtttaatca atattgggat
    18121 cgcgttgcca atggatatgg tctgaacgga gctgctgcgc aattcagctt atctgatgta
    18181 gacccaatta ctgctcaagt taaacaaatg cctactttag aacagttaaa atcctgggtt
    18241 cgtaataatg gcgaggcgtg aacatgagat cttatttgat cttgcggctt gctgggccaa
    18301 tgcaagcctg ggggcagccg acctttgaag gaacgcgacc taccggaaga tttccgaccc
    18361 gaagcgggtt attagggcta ctcggggctt gtcttgggat ccaacgtgat gatacttctt
    18421 cattacaggc gttatcagag agtgtgcaat ttgcagtgcg ctgcgatgaa ctcattcttg
    18481 acgatcgtcg tgtgtctgta acggggttgc gtgattacca tacagtcctt ggagcgcgag
    18541 aagattaccg tggtttgaaa agtcatgaaa cgattcaaac atggcgcgaa tatttatgtg
    18601 atgcctcctt taccgtcgct atctggttaa caccacaggc aacgatggtt atgtcagaac
    18661 ttgaaaaagc agtattaaag cctcggtata caccgtatct ggggcggagg agttgtccgc
    18721 taacacaacc gctttttctc gggacatgtc aggcgtcgga tcctcagaag gcgctattaa
    18781 attatgagcc cgttggcggc gatatttata gtgaggaatc agttgacggg catcatttaa
    18841 aatttacggt gcgcgacgaa ccgatgatca ccttacctcg acaatttgct tcccgagaat
    18901 ggtatgtgat taaaggaggt atggatgtat ctcagtaaag tcatcattgc cagggcctgg
    18961 agcagggatc tttaccaact tcaccaggga ttatggcatt tatttccaaa cagaccggat
    19021 gctgctcgtg attttctttt tcatgttgag aagcgaaaca caccagaagg ctgtcatgtt
    19081 ttattgcagt cagcgcaaat gcctgtttct actgccgttg cgacagtcat taaaactaaa
    19141 caggttgaat ttcaacttca ggttggtgtt ccactctatt ttcggcttcg ggcaaatccg
    19201 atcaaaacta ttctcgacaa tcaaaagcgc ctggacagta aagggaatat taaacgctgt
    19261 cgggttccgt taataaaaga agcagaacaa atcgcgtggt tgcaacgtaa attgggcaat
    19321 gcggcgcgcg ttgaagatgt gcatcccata tcggaacggc cacagtattt ttctggtgat
    19381 ggtaaaagtg gaaagatcca aacggtttgc tttgaagggg tgatcaccat caacgacgcg
    19441 ccagcgttaa tagatcttgt acagcaaggt attgggccag ctaaatcgat gggatgtggc
    19501 ttgctatctt tggctccact gtgattgagg tgtaataaaa tgacctggct tccccttaat
    19561 cccattccac tcaaagatcg cgtctccatg atctttctgc aatatgggca gatcgatgta
    19621 atagatggcg cgtttgtact tatcgacaag acagggatcc gcactcatat tcctgttggc
    19681 tcggttgcct gcatcatgtt ggaacctggt acacgggttt cgcatgcagc tgtacgcctg
    19741 gctgcgcaag ttggaacatt attggtatgg gtgggggaag cgggcgttcg tgtttatgct
    19801 tctggtcagc ctggtggtgc gcgttcagat aagctgctct atcaggcaaa acttgctctg
    19861 gatgaagatt tgcgtctgaa ggtcgtacgt aaaatgtttg aacttcggtt tggagaacct
    19921 gcgcctgccc ggcgctccgt agagcaactc agaggtatag aaggaagtcg tgtgcgagca
    19981 acctatgtac ttctggcgaa gcaatacggg gtgaaatgga atggacgtcg ttacgatccg
    20041 aaagactggg aaaagggcga tacggtcaac caatgcatta gcgctgcaac ttcttgttta
    20101 tatggcgtaa ctgaagcggc gatacttgca gctggttatg caccagctat tgggtttgtg
    20161 catacaggaa agcctctttc ctttgtttac gatatcgcgg acatcattaa atttgacact
    20221 gttgtaccga aagcttttga gatagcgcgt cgtattcctg gtgagccgga ccgggaagtc
    20281 cgtttggcgt gcagggatat ttttcgcagt agtaaaacat tagtcaaatt gattccgctt
    20341 atagaggacg tgcttgctgc tggggaaata cagccgccgg ccccacctga agatgcgcag
    20401 cctgttgcta ttccacttcc cgtttctctg ggagatgccg gacatcggag tagctgagat
    20461 gagtatgttg gtcgtggtca ctgaaaatgt accttcgcgc ttacgaggca gattagccat
    20521 ctggttgttg gaggtacgtg caggggtgta tgtaggtgat gtatccgcaa gagtccgtga
    20581 aatgatctgg gaacaaatat ctggactggc ggaagaaggc aatgtggtga tggcatgggc
    20641 tacgaatacg gaatcgggat ttgagttcca gacatttggg gtaaacaggc gtactccggt
    20701 agatttggat ggtttaaggt tggtctcttt tttacctgtt taaaaacaaa gaattagctg
    20761 atctttaata ataaggaa
//
LOCUS       DAZKGF010000088        20368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZKGF010000088 DAZKGF010000000
VERSION     DAZKGF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.088"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(365..1561)
                     /gene="hemY"
                     /locus_tag="ACHOQV_002960"
     CDS             complement(365..1561)
                     /gene="hemY"
                     /locus_tag="ACHOQV_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFP9631301.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1564..2763)
                     /gene="hemX"
                     /locus_tag="ACHOQV_002961"
     CDS             complement(1564..2763)
                     /gene="hemX"
                     /locus_tag="ACHOQV_002961"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFP9631302.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQADAP
                     AAAPQGE"
     gene            complement(2785..3525)
                     /gene="hemD"
                     /locus_tag="ACHOQV_002962"
     CDS             complement(2785..3525)
                     /gene="hemD"
                     /locus_tag="ACHOQV_002962"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFP9631303.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CVIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3522..4463)
                     /gene="hemC"
                     /locus_tag="ACHOQV_002963"
     CDS             complement(3522..4463)
                     /gene="hemC"
                     /locus_tag="ACHOQV_002963"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFP9631304.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYESLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4850..7396
                     /gene="cyaA"
                     /locus_tag="ACHOQV_002964"
     CDS             4850..7396
                     /gene="cyaA"
                     /locus_tag="ACHOQV_002964"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFP9631305.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLTQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     CNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7436..7756)
                     /gene="cyaY"
                     /locus_tag="ACHOQV_002965"
     CDS             complement(7436..7756)
                     /gene="cyaY"
                     /locus_tag="ACHOQV_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFP9631306.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7804..9861)
                     /locus_tag="ACHOQV_002966"
     CDS             complement(7804..9861)
                     /locus_tag="ACHOQV_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631307.1"
                     /translation="MINTSRQDGFMHFRNVVCLLSCSLFLSSAWGCRLSAPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGTR
                     TDDNGGENSLDIKTLHQVKFRSPWKPDFLGLILRDANFLYVDGHRLADPESFRVLAQK
                     SWDQRGKFSTTFNPCVAVPFGPWDTLARTRTKIIINGEQLKADPETFTVVRWMPGSLL
                     TWRDKSGLHRKVLNQGNLDKDRANSCAAFTLQEHSVSWRKGPECQQEVLPGLDPEQFH
                     PLSNTVAQYQDKLYTIKKTGFGENRLNIVTLDNPGLVIDKRFNAGRTHGYLLTKLPAD
                     GEEEDGLQVFDSSGPLILMDYRVPDEHEAHLSDSPHYKKWYAHDDRYVYAFDGSQLWR
                     YPTPNTKAVRVKWKTKDSGYGYWANYRSGELDGRLLEDGAFIPTGIPYNSSVNIAQYS
                     DGEGSFLVGETDVSWRKLRSTDQQWSQWEKLPDIDPKAFHPITDRVAQYKNHLYIAKL
                     SPFGEDTLEIITLDSSAPFLKQRINAGKGHGYFMRSSRLRDDIQIFAIDGPLKTTERF
                     AYDNRYVYTWIGSQLYRTASPCPARTRNLDQNMFSSNDDIIILTTEEECHKTPDIEQT
                     LKL"
     gene            complement(10203..10757)
                     /locus_tag="ACHOQV_002967"
     CDS             complement(10203..10757)
                     /locus_tag="ACHOQV_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFP9631308.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSITCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGENYLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11099..11302
                     /gene="yifL"
                     /locus_tag="ACHOQV_002968"
     CDS             11099..11302
                     /gene="yifL"
                     /locus_tag="ACHOQV_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9631309.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVEM
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11339..12163
                     /gene="dapF"
                     /locus_tag="ACHOQV_002969"
     CDS             11339..12163
                     /gene="dapF"
                     /locus_tag="ACHOQV_002969"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP9631310.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12160..12867
                     /gene="yigA"
                     /locus_tag="ACHOQV_002970"
     CDS             12160..12867
                     /gene="yigA"
                     /locus_tag="ACHOQV_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFP9631311.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDCWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12864..13760
                     /gene="xerC"
                     /locus_tag="ACHOQV_002971"
     CDS             12864..13760
                     /gene="xerC"
                     /locus_tag="ACHOQV_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP9631312.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13760..14476
                     /gene="yigB"
                     /locus_tag="ACHOQV_002972"
     CDS             13760..14476
                     /gene="yigB"
                     /locus_tag="ACHOQV_002972"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFP9631313.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14560..16722
                     /gene="uvrD"
                     /locus_tag="ACHOQV_002973"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14560..16722
                     /gene="uvrD"
                     /locus_tag="ACHOQV_002973"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFP9631314.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(16767..17669)
                     /locus_tag="ACHOQV_002974"
     CDS             complement(16767..17669)
                     /locus_tag="ACHOQV_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFP9631315.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LKQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(17752..18516)
                     /gene="yigE"
                     /locus_tag="ACHOQV_002975"
     CDS             complement(17752..18516)
                     /gene="yigE"
                     /locus_tag="ACHOQV_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFP9631316.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVG
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            18678..18734
                     /gene="ysgD"
                     /locus_tag="ACHOQV_002976"
     CDS             18678..18734
                     /gene="ysgD"
                     /locus_tag="ACHOQV_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFP9631317.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18886..19836
                     /gene="corA"
                     /locus_tag="ACHOQV_002977"
     CDS             18886..19836
                     /gene="corA"
                     /locus_tag="ACHOQV_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFP9631318.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19872..>20368)
                     /locus_tag="ACHOQV_002978"
     CDS             complement(19872..>20368)
                     /locus_tag="ACHOQV_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFP9631319.1"
                     /translation="KSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVVD
                     HLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDLY
                     CFPTIKSNDNCIVKNYLMVINEYPYRNTHFFIADNNEYQLTQENKIERVFNPHDFEKD
                     NFQQ"
BASE COUNT         4771 a         5266 c         5282 g         5049 t
ORIGIN      
        1 aaatattcac tcaggaagtt attactcagg aagcaaagag gattacagaa ttatctcata
       61 acaagtgtta agggatgtta tttcccggtt ctctgtggca taataaacga gtagatgctc
      121 attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc agagccgttt
      181 acggtgctta tcgtccactg acagatgtcg cttctgcctc atcagacacc atggacacaa
      241 cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg ctccgtaata
      301 agagcaggcg tttttttatg tatcagaaag gtcccggagg agcttgcctc cgggtgagaa
      361 agagctactg tggcgggttg ttctgcaacg ttaacatcaa accgtcgcga cgcatcgctg
      421 cggcttcttc cggcttgtgc agtctgtcca gcgcgtcggc aagccatgcg taatcgtagg
      481 cgtccggacg ttgtttcagc gctgcgcgga aggcgagcga tgcttcctgc cattcgccgt
      541 gcttcatcag cgactgaccc agtgtgctcc acaacagcgg gcgatcgccc acgtttttga
      601 tctgttggcg cagcactttt tcaagctgtt ccgggttatt ggttttcaga cgcgggatcg
      661 gcagcagcag gcgatcgtca tactggcgtt tcaggccgtc gatgataatt tgctgggcag
      721 tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgtaac gccacctgat
      781 gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcacta ccgttatcgg
      841 cacgcgcctg atccatcagg ccaatccatg cctgttgttc cagcattgca cgatgttctt
      901 catcaccaac atgggctttc gccattgatg ggatgatatc cagcagcgaa ctccatgcac
      961 cagtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt ggcgtaactt
     1021 ccagcagctt atcaacgccg tgacgtgctg catggttttc attacgggcc agttgcagac
     1081 gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctccgct gcgcgttcca
     1141 gatgttggtt ggcgcgtgct tcatcaccac gttgttgcgc ggcttcggct gccagtagat
     1201 agttcaccac cggttgttca gcgtgatcgg catttttcgc catcagcttt tcaacttgct
     1261 gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgt gcacggcgac
     1321 gcttacgtcc gacaaaccac ccacgggtgt gcgcgcctgt gcggaagatt cgccgcagta
     1381 gccactcaat ggcaaacagc actaccatcg ccagaatcaa aataatcgcg aggcccgtga
     1441 cgctagtttc aatattgtag ttgtcggtct ggatcagcac gtaaccctga tggccggcaa
     1501 tcatcgggcc aaccacgatc cccgcaatca gcaacacaaa gagcaataac acttttagca
     1561 tggttattct ccttgcggcg cggctgccgg agcatcagct tgcggtgcag gtgcaggtgc
     1621 aggtgcaggt gcaggtttag cttccgttgt ccccgctgcc ggttgtgcca gcaggttacg
     1681 cacgcgagtt tgcatcagtt tttccagcat cgcctggctt tgcagggttt ccggaagatc
     1741 catcgagata ttttgctggc ttaactggtc cacctcgtcg aggaacgctt tggtggtggc
     1801 atcatcagta tcgtagtaag cacgtaccca ggtggagacg ttctccagcg cctggcgata
     1861 agtctcttcc tggtgacgcg gtacagcttg tgctgcgacc agcaggcgag agcgaatatt
     1921 ttcgcgcaga tagatatcct gatttggcgc taacagcggt acggcggtgt catcacgacg
     1981 gcgaatcgta atgaagttgt ccataaagtt ctgccagctt ttttgcagat tgatacgcca
     2041 ttcgctgatg gaactggaaa gctcttcacc gtcggaatcc atcggcgaac catcgctgtc
     2101 attatcagcc agacgcagat tatctacctg atttgaaagc tgattaagct taaggatgat
     2161 accgtcataa tccacctgcg ataccgcaga aaggctggcg atatcatcgg taattgcccg
     2221 acgaacggta atcagactcg ggtcattcat atccgccagg ctggcatctg cacttttcag
     2281 caacgctgca gcggtcgtga cgtcttgatc gctccacagc ttccgtcctg cgagtttcac
     2341 caggaaatca gcctgagcca gcagccaggt tttagcatcg ctgccggaaa tagtcgcgac
     2401 cttttgttgg acttcatcca gctgttttgc cagcgtttct tgctgacgat tcgcctgctc
     2461 aagctgtaca gcttgttgct tgataatgcc ttccagctca gctttttggc tctcctgggc
     2521 tttttgcagt gccgtaagtt ggttagccag ggcatcgctg gtggcagtct gattgacggc
     2581 ctgttgttta ccccagccat acaaaccgac gcccgccgcc agagcaatag cgatagccac
     2641 cgcgctgaga atcaatgcgg tattgttctt actctttttt tctgttgcga caggttgtga
     2701 cgtggtgtcc acggcctccc tggtctcttc aaccacggcg gaggtttttt cttgttccgt
     2761 cattatggct tcctgttatg agagttattg taatgcccgt aaaagcgcat cgttgtcagc
     2821 gttatcggcg accttaatgt cttgccagcc cagttcccgg gcgagtttcg ccaaacgctc
     2881 actgacgacc aatagtcgac agcgtagtaa ccagtgctca cgataccatt gtgggatcag
     2941 cgaccagagt tgctgcaaca tttcaccgct ggtaacaacg accgtcgtca cctcgcggga
     3001 ttgccagcgc atcgcttctt ctgcaccatc gtaatggatt acgcatcgtt gataacattc
     3061 acaaaaagtg acctcagcac cgcgcgccgt cagggtatcc ccaattagct cacgaccgcc
     3121 attgccacgt aatatcagcg cacgtttgcc cgcaatattt tgtaattcag gtaattgtag
     3181 caagacttcg ctgatttccc gatcctgcgg gtagagaatc ttctgtccgc ttacggtatg
     3241 tagtgccagt gcggtggtgc gtccaatggc gaaataagta ggtagtcggg gccatttacg
     3301 atcttgctga tgcagctgtg attgggcaaa agcaaccgcg tgttgcgaga gggcaaacaa
     3361 cagatcgctc tcccccagcg ccgccagttg atcagcaagt tgtggtaatt gtcgacccgg
     3421 agaaaactca atcagtggaa aatgccaggc cacctgcccc agtgtgcgca gacggctcac
     3481 taactcttct ccagcgggag acgggcgggt gacaaggata ctcatgccgg ggcgtctcca
     3541 ttatagactt cagcgaggat ctcgcgcgcg ccgttattca gtagctcttc tgccagcgaa
     3601 atccccattt gttcggcatc ttgcggcgca ccgcggcgtt caccgcgaat aatctgcgaa
     3661 ccgtccggcg cgccgaccag cgcacgcagc cagatttcac catcaataag ctcggcgtag
     3721 ctaccaattg gcacctgaca tccgccttcg agacgggtat tcatggcgcg ttctgcggta
     3781 acgcgcagtg cagtttcgtg gtgattcagc gcggcaagca gctcgcgagt gcgtgtatca
     3841 tcaaggcggc attcaatacc gaccgcacct tgtcctaccg ccggaagaga aatctcgggt
     3901 ggtaacgcgg cgcgaatacg tgactccaga cctaaacgtt ttagtccggc tacggcaaga
     3961 atgatggcat cgtattcgcc gttatccagt ttactcaggc gagtgccgac gttgccgcgc
     4021 agggagcgga taatcagatc cgggcggcgt tcagccagtt ggcactggcg acgtaaactg
     4081 gacgtcccga cgatactgcc tgccggtaac gcatccagac tttcatagtt attggacaca
     4141 aaggcatcgc gaggatcttc acgctcgcaa atggtgacca gccccagacc ttgcgggaat
     4201 tcaaccggca catctttcat tgaatgtacg gcgatatcgg cgcgattctc caggagcgcg
     4261 acttccagct ctttaacaaa taagcctttt ccgcctactt tcgccagcgg cgtatcaaga
     4321 atcacatcgc cgcgcgtcac catcggtacc agttcaacga ccaggcccgg atggctcgcc
     4381 atcaacttgt ctttgacata gtgtgcctgc cagagtgcaa gtgggctttg gcgtgtggca
     4441 attcttaaaa cattgtctaa catgcttgtt accgtcatta tcatccgtgg tccatcctaa
     4501 catccttgcc agagcgatgt cagtgttgtg gtgaaacgta gacgcctgcg caaaccgtaa
     4561 aatgaggtct ggcagtggat cctgacaggc gtttcacgcc gtcgtaataa ggaatttaca
     4621 gagaataaac ggtgctacac ttgtatgtag cgcatctttc tttacggtca atcagcaagg
     4681 tgttaaattg atcacgtttt agaccatttt ttcgtcgtga aactaaaaaa accaggcgcg
     4741 aaaagtggta acggttacct ttgacatacg aaatatcccg aatgccgcgt gttaccgttg
     4801 atgttggcgg aatcacagtc atgacgggta gcaaatcagg cgatacgtct tgtacctcta
     4861 tattgagact ctgaaacaga gactggatgc cataaatcaa ttgcgtgtgg atcgcgcgct
     4921 tgctgctatg gggcctgcat tccaacaggt ctacagtcta ctgccgacat tgttgcacta
     4981 tcaccatccg ctaatgccgg gttaccttga tggtaacgtt cccaaaggca tttgccttta
     5041 cacgcctgat gaaactcaac gccactacct gaacgagctt gaactgtatc gtggaatgtc
     5101 agtacaggat ccgccgaaag gtgagcttcc aattactggt gtatacacca tgggcagcac
     5161 ctcgtccgta gggcaaagtt gttcctctga cctggatatc tgggtctgtc atcaatcctg
     5221 gctcgatagc gaagagcgcc agttactaca acgtaaatgc agcctgctgg aaagctgggc
     5281 cgcctcgctg ggcgtggaag ttagcttctt cctgattgat gaaaatcgct tccgtcataa
     5341 tgaaagcggc agcctggggg gcgaagattg tggctccacc cagcatatac tgctgcttga
     5401 cgaattttac cgtaccgccg tgcgtctcgc tggtaagcgt attctgtgga atatggtgcc
     5461 gtgcgacgaa gaagagcatt acgacgacta tgtgatgacg ctttacgcgc agggcgtgct
     5521 gacgccaaat gaatggctgg atctcggtgg cttaagctcg ctttctgctg aagagtactt
     5581 tggtgccagc ctttggcagc tctacaagag tatcgattcc ccatacaaag cggtgctgaa
     5641 aacactgctg ctggaagcct attcctggga atacccgaac ccacgtctgc tggcgaaaga
     5701 tatcaaacag cgtttgcacg acggcgagat tgtgtcgttt ggcctcgatc cttactgcat
     5761 gatgctggag cgtgttactg aatacctgac ggcgattgaa gatttcactc gtctggattt
     5821 agtacgccgc tgcttctatt taaaagtgtg cgaaaaactc agccgtgaac gcgcctgcgt
     5881 aggctggcgt cgtgaagtgc taacccagtt agtgaaagag tgggaatggg acgatgctcg
     5941 tctggcaatg ctcgataacc gcgctaactg gaagattgat caggtacgtg aggcgcacaa
     6001 cgagttgctc gacgcgatga tgcagagcta ccgtaatctg attcgctttg cgcgccgcaa
     6061 taaccttagc gtctccgcca gcccacagga tattggcgta ctgacgcgta aactgtatgc
     6121 cgcgtttgaa gcgttaccgg gtaaagtgac gctggtaaac ccgcagattt cgcccgatct
     6181 ctcggaaccg aatctgacct ttatttatgt gccgccgggc cgggctaacc gttcaggttg
     6241 gtatctgtat aaccgcgcgc caaatattga gtcgatcatc agccatcagc cgctggaata
     6301 taaccgttac ctgaataaac tggtggcgtg ggcatggttt aacggcctgc tgacctcgcg
     6361 tacccgcttg tatattaaag gcaacggcat tgtcgatttg cctaagttgc aggagatggt
     6421 cgccgatgtg tcacaccatt tcccgctgcg cctgcctgca ccgacgccaa aagcgctcta
     6481 cagcccgtgt gaaattcgcc atctggcgat tatcgttaac ctggaatatg acccgacagc
     6541 tgcgttctgc aatcaggtgg tgcattttga tttccgtaag ctggacgtct tcagctttgg
     6601 cgagaatcaa aattgcctgg taggtagcgt ggacctgctg taccgcaact cgtggaacga
     6661 agtgcgtacg ctgcacttca acggcgagca gtcgatgatc gaagccctga aaaccattct
     6721 cggtaaaatg catcaggacg ccgcaccgcc agatagcgtg gaagtcttct gctacagcca
     6781 gcatctgcgc ggattaatcc gtactcgcgt gcagcaactg gtttctgagt gcattgaact
     6841 gcgtctttcc agcacccgcc aggaaaccgg gcgcttcaag gcgctgcgcg tttctggtca
     6901 aacctggggc ttattcttcg aacgcctgaa tgtatcggta cagaaactag aaaatgccat
     6961 cgagttttat ggcgctattt cgcataacaa actgcacggc ctgtcagtgc aggttgaaac
     7021 caatcacgtc aaattaccgg cggtggtgga cggctttgcc agcgaaggga tcatccagtt
     7081 cttcttcgaa gaaacgcaag acgagaatgg ctttaatatc tacattctcg acgaaagcaa
     7141 ccgggttgag gtgtatcacc actgcgaagg cagcaaagag gagctggtgc gtgacgtcag
     7201 tcgcttctac tcgtcatcgc acgaccgttt tacctacggc tcaagcttca tcaacttcaa
     7261 cctgccgcag ttctatcaga ttgtgaaggt tgatggtcgt gaacaggtga ttccgttccg
     7321 caccaaatcg attggcaaca tgccgcctgc caatcaggat cacgacacgc cgctattaca
     7381 gcagtatttt tcgtgatgag cgtgccggaa agcgaggctt atccggcatg caatcttagc
     7441 ggaaactgac tgtttcaccc gcttgctgcg ttgccgcctg ttccagcaaa tcccagaagg
     7501 tttcgccgct acgatcgcaa atccactcat cgcctttcag atcaaaatgg tagccgccct
     7561 gtttggttgc cagccatacc tggtgcagcg gttcctggcg gttgataatg attttgctcc
     7621 cgttttcaaa ggtaatggtc agtacaccgc cgttgatttc gcagtcgata tcgctatcgc
     7681 catcccagtc gtccagacgc tcttcaatgg tcagccacag ttgatcagcc aggcgatgaa
     7741 attcactgtc gttcattgtt gtatcctgtt tttaagtgat ggcggcagta tagcggcatg
     7801 gggtcagagc ttcaaagttt gctcaatgtc tggtgttttg tggcactctt cctccgtcgt
     7861 caagatgatg atgtcatcat tggatgaaaa catgttctga tccagattac gtgtcctggc
     7921 cggacagggc gatgcggttc gataaagttg gctaccgatc caggtataaa catagcgatt
     7981 gtcataagcg aaacgttcgg tggttttcaa tggtccatca atagcaaaaa tttgaatatc
     8041 atcgcgtaaa cgggaagagc gcataaagta accatggcct ttcccggcgt taatccgttg
     8101 tttcaggaaa ggtgcagaag agtcgagcgt tattatttcc agggtgtctt caccaaaagg
     8161 agacaatttc gcaatgtaca gatgattttt gtactgcgcg acacgatcgg tgatgggatg
     8221 aaatgctttc gggtcaatat cggggagttt ctcccattga ctccattgct ggtctgtaga
     8281 gcgaagctta cgccagctaa catcggtttc gccgacgaga aaagagcctt cgccatcaga
     8341 atattgagca atgttaacgg atgagttata ggggattccc gttggaataa acgcaccatc
     8401 ctcaagaagt ctgccgtcaa gctcaccact acggtagttt gcccaatagc catatcctga
     8461 atctttggtt ttccatttca cccgcacggc tttggtattg ggagttgggt agcgccaaag
     8521 ctgtgagcca tcgaatgcgt aaacataacg atcgtcgtgt gcataccatt tcttgtaatg
     8581 ggggctatcg ctaaggtgag cctcatgctc gtcaggaaca cgataatcca tcaggataag
     8641 cggcccggaa gaatcgaata cctggaggcc atcttcttct tcaccatcag ctggtaattt
     8701 ggtcaataaa tagccgtgag ttcttcccgc gttaaagcgt ttatctatca ccaaaccagg
     8761 attatccagt gtgacgatat ttagcctatt ttcgccaaat cccgtttttt tgatggtgta
     8821 cagcttgtcc tgatactgag caacggtatt actaagagga tgaaattgtt ctggatcgag
     8881 gccgggtagg acttcttgct ggcattctgg ccctttgcgc catgaaacag agtgttcctg
     8941 caaagtgaac gccgcgcagc tatttgccct atctttgtcc agatttccct gattcagcac
     9001 cttccggtgc aggccgcttt tatctcgcca ggttaataac gaaccgggca tccagcgtac
     9061 gacagtaaat gtctctgggt cggctttaag ctgttcgcca ttgattatga ttttggtccg
     9121 tgtacgggct aacgtatccc aggggccaaa tggtacggca acgcaggggt taaacgtggt
     9181 ggaaaacttt cctcgctgat cccaggactt ctgcgccagc acacggaaac tctcggggtc
     9241 agcgaggcga tgtccatcga catataagaa attagcgtca cgtaatatca gccccagaaa
     9301 gtcaggtttc cagggggaac ggaatttcac ctgatgcagc gttttaatat ctagactatt
     9361 ttctccgcca ttgtcatcgg tgcgcgtgcc ttcaaaatag agactgtgtt tatctgcggc
     9421 aaaatcaccc catgccttga acgaggcgac atcgacggga ggtttgtctg gcgggttttg
     9481 cacaacttca cccgcgtaga ggatatagcg accatcgcta tgccaggagt aatgaggata
     9541 ccaggcctgt gcgccttcat agaggtagtc ggttgtcaga tcagtcagag gaggtacttt
     9601 atcccatttg tctagtgttg tcgggtctat taataaattg ggtaaacggc gcaaactttt
     9661 gtaattcacg tcaggaagag aaagatttgt ttctttataa gggcgcaggt ataccacgcc
     9721 ttttccctgt ttctggtaaa tattatgttc aggcgcggaa agccgacatc cccatgccga
     9781 cgataaaaac aacgaacaag acaacaaaca aaccacattg cgaaagtgca taaagccatc
     9841 ctggcgcgag gtgttgatca cgaaactgct agcagaacat aaatcctcac aagtaagcgt
     9901 aactctcgca gcaaatcgtc acttttcaga atccataaag gtcatcgcta ttttctgata
     9961 gttgctggaa taaacgtgat attttcattc acgatataaa cacaatcccg gaactgcccc
    10021 atagtacggc taatagcata aaaatattcc ggattgaccc ctggaaggga ggcaatgtgg
    10081 gattccgccc cccgttgcct tctttattgt ctttcacgag ttgtacccag gttccaccac
    10141 gggtatctaa cattcgccac attattacgc tttctgccga acgtgatttt tatagaaatc
    10201 tatcaattca ttttccctgt accataaagt ttatccttca catcctgccg aacgcgggtc
    10261 ttgtaccccc cgttgtgaag atcttcatac ggagaaaaag aatctttact ccaccccagc
    10321 aacactttat cgccaggcat aaaatgcacg tgcaggtaat tttctcccca ttctcttttt
    10381 gggagaggta acgtcatatt gcgtttctcc agacgcattc cagcattata ctgtgtacga
    10441 gtagtactta atgtccatat aacctctgcc gtatccccac tgacatcacc gcaacaggtg
    10501 attgaaccgc caccattcgc aaaagcattc gcgcccgcca cgccgttaac actgaatccc
    10561 agtatcggac ggtcaatttc actgtggatt tccagtatca ctccacctct tggtcctccc
    10621 catactgcct gtcctatatt ccacaacacg accagtactg gaacgacaat taacgtccgc
    10681 catacccagc gcccccagcg ggcataaccg cgcgtaagct tttggtcaaa tttttccagt
    10741 tgggtaaata gccccatcga ttttattccc tcttctcacc tggttggcaa agttgcacgt
    10801 cactggcgac taaggtcaga caacgaaatt tcgtggtacg ccactctctt aaaagtccca
    10861 tgcaggcgat aagaacaaga caacaaacaa accacattgc gatagtgcat aaagccatcc
    10921 tggcgcgagg tgccgatcac gaaactacca gcaaaacata aatcgccacg tgtaagcgtt
    10981 atactcgcag catttcctca cttttcagac ttcataaaga gtcgctaaac gcttgctttt
    11041 acgccttctc ctgcgatgat agaaagcaga aagcgatgaa ctttacaggc aatccataat
    11101 gaaaaacgtg tttaaggcac tcactgtatt acttactctc ttcagcctga cgggctgcgg
    11161 tctgaaaggt ccgctctatt tcccgcctgc agataaaaac gcaccgccgc cgaccaaacc
    11221 ggtagagatg caaacgcaat ccacggtgcc ggataaaaac gaccgcgcca ctggcgatgg
    11281 tccatcccag gtgaattact aaaagtcagt ttctgtaccc gcgtgattgg agtaaatgat
    11341 gcagttctcg aaaatgcatg gccttggcaa cgattttatg gtcgtcgacg cggtaacgca
    11401 gaatgtcttt ttttcaccgg agctgattcg tcgcctggcg gatcggcacc tgggggtagg
    11461 gtttgaccag ctgctggtgg ttgagccgcc gtatgatcct gaactggatt ttcactatcg
    11521 cattttcaat gctgatggca gtgaagtggc gcagtgcggc aacggtgcgc gctgctttgc
    11581 ccgttttgtg cgcctgaaag ggctgactaa taagcgcgat atccgcgtca gcaccgccaa
    11641 cgggcggatg gttctgaccg tcaccgatga tgatctggtc cgcgtaaata tgggcgaacc
    11701 caacttcgaa ccttccgccg tgccgtttcg cgctaacaaa gcggaaaaga cctatattat
    11761 gcgcgccgcc gagcagacaa tcttatgcgg cgtggtgtcg atgggaaatc cgcattgcgt
    11821 gattcaggtc gatgatgtcg ataccgcggc ggtagaaacg cttggtcctg ttctggaaag
    11881 ccacgaacgt ttcccggagc gcgccaatat cggttttatg caagtggtta agcgcgagca
    11941 tattcgttta cgcgtttatg agcgtggggc aggagaaacc caggcctgcg gcagcggcgc
    12001 gtgtgcggcg gttgcagtag ggattcagca aggtttgctg gccgaagaag tacgcgtgga
    12061 actccccggc ggtcgtcttg atatcgcctg gaaaggtccg ggtcacccgt tatatatgac
    12121 tggcccggcg gtacatgtct acgacggatt tattcatcta tgaagcaacc aggggaagaa
    12181 ctgcaggaaa cactcacgga gcttgatgac cgggcggttg tcgattatct gattaaaaat
    12241 cctgagtttt ttatccgcaa tgcgcgcgca gtagaagcga tacgtgtgcc gcatccggta
    12301 cgcggcaccg tttcgttggt cgagtggcac atggcccgcg cacgtaatca cattcatgtt
    12361 ctggaagaga acatggcgtt gttgatggag caggctatcg ccaacgaagg cctgttttat
    12421 cgcttactct acttgcagcg tagtctcacc gccgccagca gtctcgacga tatgctgatg
    12481 cgctttcacc gctgggcgcg cgatctcggc ctggcgggtg cgagcctgcg cctgttcccg
    12541 gattgctggc gtttaggtgc gccgtcgaac cacactcatc tggcattaag ccgtcagtct
    12601 ttcgaaccgc tgcgtattca gcgtttgggg caggaacagc actatcttgg gccgcttaac
    12661 ggaccggagc tgctggtggt gctaccggaa gcgaaagcgg tgggatcggt ggcgatgtcg
    12721 atgctgggaa gcgatgctga tttgggtgtc gtgctgttta ccagtcgcga tgccagtcac
    12781 tatcaacaag ggcagggaac gcagttactt catgaaattg cgctgatgtt gccggagctt
    12841 ctggagcgtt ggattgaacg cgtatgaccg atttacacac cgatgtagaa cgctacctac
    12901 gttatctgag cgtggagcgt cagcttagcc cgataaccct acttaactat cagcgtcagc
    12961 ttgaggcgat catcaatttt gccagtgaaa acggcctgca aagctggcag caatgtgatg
    13021 tgacgatggt gcgcaatttc gctgtacgca gccgccgtaa agggctggga gctgcaagtc
    13081 tggcgttacg gctttctgcg ctacgtagct tttttgactg gctggtcagc cagaacgaac
    13141 tcaaagccaa cccggcgaaa ggtgtttcgg caccgaaagc gccgcgtcat ctgccgaaaa
    13201 acatcgacgt cgatgatatg aatcggctgc tggatattga tatcaacgat cccctcgccg
    13261 tgcgcgatcg ggcaatgctg gaagtgatgt acggcgcggg tctgcgtctt tccgaactgg
    13321 tggggctcga tatcaaacac ctcgacctgg agtccggcga agtgtgggtg atgggaaaag
    13381 gcagcaaaga gcgccgcctg ccgattggtc gcaacgctgt ggcgtggatt gagcactggc
    13441 ttgatttgcg cgacctgttt ggtagcgaag acgacgcgct ttttctgtcg aaactgggca
    13501 agcgtatctc cgcgcgtaat gtgcagaaac gctttgccga atggggcatc aaacaagggc
    13561 tgaataatca cgttcacccg cataaattac gtcactcgtt cgctacgcat atgctggagt
    13621 cgagcggcga tcttcgtggt gtgcaggagc tgctgggtca tgccaacctc tccaccacgc
    13681 aaatctatac tcatcttgat tttcaacacc ttgcctcggt gtacgatgcg gcgcatccac
    13741 gcgccaaacg ggggaaataa tgcgttttta ccggcctttg gggcgcatct cggcgctcac
    13801 ctttgacctg gatgataccc tttacgataa ccgtccggtg attttgcgca ccgagcgaga
    13861 ggcgcttacc tttgtgcaaa attatcatcc ggcgctgcgc agcttccaga atgaagatct
    13921 gcaacgcctg cgccaggcgg tacgggaagc ggaacccgag atttatcacg acgtgacgcg
    13981 ctggcgtttt cgttcgattg aacaagccat gctcgacgcc gggctgagtg ccgaagaagc
    14041 cagtgcaggc gcacacgcag caatgatcaa ctttgccaaa tggcgcagcc gaatcgatgt
    14101 cccgcagcaa acccacgaca ccttaaaaca gctggcgaag aaatggccgc tggtggcgat
    14161 caccaacggt aacgcccagc cggagctgtt tggtttaggg gattattttg agtttgtgtt
    14221 gcgcgctggc ccgcacgggc gctcaaaacc gttcagcgat atgtacttcc tggcggcgga
    14281 aaaactcaac gtgccgatcg gcgagatctt acatgttggg gacgatctca ccactgacgt
    14341 gggtggggca attcgcagcg gaatgcaggc ctgttggatc agaccggaaa atggcgatct
    14401 gatgcaaacc tgggacagcc gtttactgcc gcatctggaa atttcccggt tggcatctct
    14461 gacctcgctg atataatcag caaatctgta tatataccca gctttttggc ggagggcgtt
    14521 gcgcttctcc gcccaaccta tttttacgcg gcggtgccaa tggacgtttc ttacctgctc
    14581 gacagcctta atgacaaaca gcgcgaagcg gtggccgcgc cacgcagcaa ccttctggtg
    14641 ctggcgggcg cgggcagtgg taagacgcgc gtactggtgc atcgtatcgc ctggctgatg
    14701 agcgtggaaa actgctcgcc atactcgatt atggcggtga cgtttaccaa caaagcggcg
    14761 gcggagatgc gtcatcgtat cgggcaactg atgggcacca gccagggcgg catgtgggtc
    14821 ggcaccttcc acgggctggc gcaccgtctg ctgcgtgcgc accatatgga cgccaatctg
    14881 ccgcaggatt ttcagatcct cgacagcgag gaccagctac gcctgctcaa acgcctaatc
    14941 aaggcgatga acctcgacga gaagcagtgg ccgccgcgcc aggcaatgtg gtacatcaac
    15001 agccagaaag atgaaggcct gcgtccacat catattcaaa gctacggtaa tccggtggag
    15061 cagacctggc agaaggtgta tcaggcgtat caggaagcgt gtgaccgcgc gggcctggtg
    15121 gacttcgccg agctgctgct acgtgctcac gagttgtggc ttaacaagcc gcatatcctt
    15181 cagcactatc gcgaacggtt taccaatatc ctggtggatg aattccagga taccaacaac
    15241 attcagtatg cgtggatccg cctgctggcg ggcgataccg gcaaagtgat gatcgttggc
    15301 gatgacgacc agtcaatcta cggctggcgt ggggcgcagg tcgagaatat tcagcgtttc
    15361 ctcaatgatt tccccggtgc tgaaactatc cgtctggagc agaactaccg ctctaccagc
    15421 aatattctga gcgccgctaa cgccctgatt gaaaacaata acgggcgtct gggtaaaaaa
    15481 ctgtggaccg atggcgcgga cggtgagcct atttccctct attgcgcttt taacgaactc
    15541 gacgaagcgc gttttgtggt taaccgtatc aagacctggc aggacaatgg cggggcgctt
    15601 gccgagtgcg cgattctcta ccgcagcaac gcccagtcgc gtgtactgga agaagcactg
    15661 ttgcaggcga gtatgccgta ccgtatttac ggcgggatgc gcttcttcga acgccaggaa
    15721 atcaaagatg cgctctcgta tctgcgcctg attgccaacc gtaatgacga cgcggcgttt
    15781 gagcgcgtgg tgaatacacc aacgcggggt attggtgacc ggacgctgga cgtggtacgt
    15841 cagacatcgc gcgatcgcca gttaacactc tggcaggcat gtcgtgaact gttgcaggaa
    15901 aaagccctcg ccggacgtgc tgccagtgcc ttacagcggt ttatggaact gatcgacgcc
    15961 ttagcgcagg aaactgccga tatgccgctg catgtacaga ctgaccgggt aattaaagac
    16021 tccggcctgc gcacaatgta cgagcaggag aaaggcgaaa aaggccagac gcgtatcgaa
    16081 aacttagagg aactggtaac ggcgacgcgc cagttcagct acaacgaaga agacgaagat
    16141 ttaatgccgc tgcaggcgtt cctctcccat gcggcactgg aagcgggcga ggggcaggcg
    16201 gatacctggc aggatgcggt gcagttgatg acgctacact cggcgaaagg tctggaattc
    16261 ccgcaggtgt ttatcgttgg tatggaagag ggcatgttcc caagccagat gtcgctggat
    16321 gaaggcgggc gtctggaaga agaacgccgt ctggcctacg ttggcgtaac ccgcgcgatg
    16381 cagaaactga cgctgaccta cgcggaaacc cgccgtctat atggcaaaga ggtttaccat
    16441 cgcccgtcgc gctttatcgg tgagttgccg gaagagtgtg tggaagaggt gcgcctgcgc
    16501 gccacggtaa gccgcccggt cagccatcaa cggatgggga cgccgatggt cgagaacgac
    16561 agcggctaca agctcggcca gcgcgtacgc cacgctaagt ttggtgaagg caccatcgtc
    16621 aatatggaag gcagcggtga gcatagccgt ttgcaggtgg cgttccaggg ccagggtatt
    16681 aaatggctgg tggcggctta cgcccggttg gagacggtgt gacgttgcgg gatgcggtgc
    16741 gccacaccgc attccgcatc agcaccttac ttagcgcact tcttcacatc aatattccag
    16801 tcagcaccag acgcaacctg atgtttctgc gcaaaattat ccatattcag cgccacggaa
    16861 acattcaata agctgttcag atacaccatc ggctggcctt ccggcacatc gccgaagctt
    16921 gcgacatacg gcattttccc tgcgtatttt tgctgggaac cttcggaaat ggtcacacac
    16981 aacgtgtcgt tgagtttgat tcctgcctgt ttcagcaact cgtcgctgat attgctccag
    17041 acgttgccgt actgaatatc gagaatcggg atattgccct tcacttcacc ttttgttgct
    17101 tttgctttct gataagaaag ttcgacaact tttgcaggaa gctctggccc cacctgttcg
    17161 aacgtaatcg ctccggaagc cagacgcgca ccagtgtagg catatacatc gcgaccatgg
    17221 aaggtatatg acttttcaga acctttcagg cggttagctt tttcgtcgat ttcccggacg
    17281 ctatcaatac ccagagtttg tgccaccaaa gtgagcgtac cgttatctgg tgaaacgaag
    17341 tattgaccgt ttttggtttt cagcactacc gatttacggt cagtgcctac gcccggatcg
    17401 accacgctaa caaacaccga accttgcggc cagtaactgg cagtctgata caggcgataa
    17461 gcgccttccc agatgttata cggtggaatt tcgtgtgtta aatcaaagat tttaagatta
    17521 tggtcaacgc cgaatgcgac ccctttcatc gccgagacgg caccatcttt caggctaaaa
    17581 tccgtttgta aaatcaaagc gttatccgca taagcggatg atgaccccag acaggttaat
    17641 aaaaataatg ttgcgagcgt ttttttcatt tttgtttccc ttcatttatc tgatgatttt
    17701 ccagctgtgt agccagcccc agtttgtctg gcctcctcga gtgtacaatg tttaaccttt
    17761 ctgctcgacg gaaatcatgg tgacaaaagg atagcgttgc cacgggattg cctcgccttt
    17821 catatacata tgtgaaattg tgccatcaag ataaagcagc tgcccaacgt tcagtttcgc
    17881 tttggcataa caggcaaaat cataaaaatt tgtcgcctgc tggctcaaca aaaacacggc
    17941 gttcccatgt ttattaatcc caacaccgtt acgaatttta cgtgaggcga cgttgggatg
    18001 aatacgcgga ttaattacac cgttttccat caacaatggc cctgactgca ccgcaaactg
    18061 aatctcttta ctggttttaa aggcatccag acgaacgatg ccgactttat ctcccgcgac
    18121 ataaaacact ccgccaggac gaataaagaa attcccttca cctgaagcga gatttaacgc
    18181 caccttctgc tgaccgtttt cgatgtacaa accgagcggc gcatagcttt catcatagat
    18241 gccgccgttc atcgccatct gcacctgacc ctgactatta atatccgcca gcagagcatg
    18301 taacgttccc cacgcttcgc cattggcttt ttgccagtac attttcaccc gctctgtttg
    18361 aggattaacg gtatacgcct gtacggtcag cgtcggatct gagggtgcgc aatcatcagc
    18421 ggcaacagca aacaagggaa gtaaggtgag ggtgagaaaa atccgtttga gattcaaggt
    18481 gatcattcct ttaccaatga atagccgatg cgccattata ggccctggat gtgggatttt
    18541 tttatcctgt tagcgacctt gacgagtacc aaaaagcgcg aagttcaact attgttctgt
    18601 ggtgttctgt tgcgtgttga cggcaaaatt ttgctggcgt aacatgcgcg cacgatcact
    18661 ctaagaggac attcgccttg gacacaccca gtagatactg gctcactatc ctgtcatcca
    18721 ggatcaactc ctaaggctat ccctttttgc tgatagcctt agcggttgtc agcgacctca
    18781 atttttcccg tcgcgctgag tcaggctgtt taatggtctg aaacccaatt tgtttctgtg
    18841 tgcccaccga actgtccgat attttaagca ttgggagtcc cggtcatgct gagcgcattt
    18901 caactggaaa ataaccgact gacccggctg gaagtcgaag agtcacaacc ccttgtaaat
    18961 gcagtatgga ttgatcttgt cgaaccggac gacgacgagc gactgcgcgt acaatctgaa
    19021 cttggtcaga gcctggcaac ccgcccggaa ctggaagaca tcgaagcatc ggcacgtttc
    19081 tttgaagacg atgacggcct gcatattcac tccttcttct tctttgaaga tgcggaagat
    19141 cacgccggta actctactgt ggcatttacc atccgtgatg gtcgtctgtt tactctgcgt
    19201 gagcgtgaac tgcctgcatt ccgtctgtat cgtatgcgcg cccgtagcca gtcaatggtg
    19261 gacggtaacg cctacgagct gctgttggat ctgttcgaaa ccaaaatcga acagttggcg
    19321 gatgaaattg aaaacatcta cagcgatctg gagcagttga gccgggtgat tatggaaggg
    19381 catcagggcg atgagtacga cgaggcgctc tccacgctgg cggaactgga agatatcggc
    19441 tggaaagttc gcctgtgtct gatggatacc cagcgtgcgc tcaacttcct ggtacgtaaa
    19501 gcacgcttac cgggtgggca actggagcag gcgcgtgaaa tcctgcgaga tatcgaatcc
    19561 ctgctgccgc ataacgaatc cctgttccag aaggtgaact tcctgatgca ggcggcgatg
    19621 ggctttatca atatcgagca gaaccgcatc atcaaaatct tctcggtggt atccgtggtg
    19681 ttcctgccac cgacgctggt cgcttccagc tacgggatga actttgagtt tatgccggaa
    19741 ctgaagtgga gcttcggcta cccaggcgcg attatcttta tgatcctcgc gggcctggca
    19801 ccgtatctgt actttaagcg gaagaactgg ttgtaatata aatgccgggt aaagattgtt
    19861 ctttatccgg cttattgctg aaaattgtct ttttcaaagt catgcgggtt gaacactcgc
    19921 tctatcttat tttcctgggt tagttgatac tcattgttat cagcaataaa aaaatgagta
    19981 ttacggtaag gatattcatt aataaccatt aaatagtttt ttacgatgca attatcattg
    20041 ctcttaatcg tcgggaaaca gtagagatct tcatacggcc cttcgatata ttttttatag
    20101 tcagtgaagc cattaccgat atcaataaca taccaggtat gccaactacc ttctggtagg
    20161 ggatgttgat ttttaatttc attctgatgc ttcagatacc aatttatttt atcgctgtca
    20221 gtccatggca gatgatcaac gaccagaaag actgaattgc cagcatcaga gtatgtatac
    20281 aaaatctgta caggacgcaa tgtccataaa ttatagatgc agcatagaag aaataaacat
    20341 aatataattt tctggagttt gcttttca
//
LOCUS       DAZKGF010000089        19867 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZKGF010000089 DAZKGF010000000
VERSION     DAZKGF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.089"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            188..859
                     /locus_tag="ACHOQV_002979"
     CDS             188..859
                     /locus_tag="ACHOQV_002979"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFP9631320.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPSDIEIIRVYTMHDADRHQDHLAVYQASMVACRAIPQILGYETPSTWLSF
                     MPQVFESVKEEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            869..2065
                     /gene="yaiP"
                     /locus_tag="ACHOQV_002980"
     CDS             869..2065
                     /gene="yaiP"
                     /locus_tag="ACHOQV_002980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9631321.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFIAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSHPSVTGT
                     EKLSEA"
     gene            2100..2624
                     /locus_tag="ACHOQV_002981"
     CDS             2100..2624
                     /locus_tag="ACHOQV_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9631322.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPT
                     GTYSLRQELIRTGD"
     gene            2626..3399
                     /gene="yaiO"
                     /locus_tag="ACHOQV_002982"
     CDS             2626..3399
                     /gene="yaiO"
                     /locus_tag="ACHOQV_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFP9631323.1"
                     /translation="MIKRTLLAAAIFSTLPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3584..3859
                     /gene="frmR"
                     /locus_tag="ACHOQV_002983"
     CDS             3584..3859
                     /gene="frmR"
                     /locus_tag="ACHOQV_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFP9631324.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3894..5003
                     /gene="frmA"
                     /locus_tag="ACHOQV_002984"
     CDS             3894..5003
                     /gene="frmA"
                     /locus_tag="ACHOQV_002984"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFP9631325.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5095..5928
                     /gene="fghA"
                     /locus_tag="ACHOQV_002985"
     CDS             5095..5928
                     /gene="fghA"
                     /locus_tag="ACHOQV_002985"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP9631326.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFAPIVSPSQVPWGQQAFTAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6155..6694)
                     /gene="yaiL"
                     /locus_tag="ACHOQV_002986"
     CDS             complement(6155..6694)
                     /gene="yaiL"
                     /locus_tag="ACHOQV_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFP9631327.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDYLMW"
     gene            complement(6796..8007)
                     /gene="mhpT"
                     /locus_tag="ACHOQV_002987"
     CDS             complement(6796..8007)
                     /gene="mhpT"
                     /locus_tag="ACHOQV_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFP9631328.1"
                     /translation="MSTRTPSSSSTRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGIGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8183..9196)
                     /gene="mhpE"
                     /locus_tag="ACHOQV_002988"
     CDS             complement(8183..9196)
                     /gene="mhpE"
                     /locus_tag="ACHOQV_002988"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFP9631329.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQVAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGIHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9193..10143)
                     /gene="mhpF"
                     /locus_tag="ACHOQV_002989"
     CDS             complement(9193..10143)
                     /gene="mhpF"
                     /locus_tag="ACHOQV_002989"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFP9631330.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10140..10949)
                     /gene="mhpD"
                     /locus_tag="ACHOQV_002990"
     CDS             complement(10140..10949)
                     /gene="mhpD"
                     /locus_tag="ACHOQV_002990"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFP9631331.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10959..11825)
                     /gene="mhpC"
                     /locus_tag="ACHOQV_002991"
     CDS             complement(10959..11825)
                     /gene="mhpC"
                     /locus_tag="ACHOQV_002991"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFP9631332.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVKAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11843..12787)
                     /gene="mhpB"
                     /locus_tag="ACHOQV_002992"
     CDS             complement(11843..12787)
                     /gene="mhpB"
                     /locus_tag="ACHOQV_002992"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFP9631333.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12789..14453)
                     /gene="mhpA"
                     /locus_tag="ACHOQV_002993"
     CDS             complement(12789..14453)
                     /gene="mhpA"
                     /locus_tag="ACHOQV_002993"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFP9631334.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14644..15477
                     /gene="mhpR"
                     /locus_tag="ACHOQV_002994"
     CDS             14644..15477
                     /gene="mhpR"
                     /locus_tag="ACHOQV_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFP9631335.1"
                     /translation="MQNNEQTAYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15554..16636
                     /gene="lacI"
                     /locus_tag="ACHOQV_002995"
     CDS             15554..16636
                     /gene="lacI"
                     /locus_tag="ACHOQV_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFP9631336.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPQALADSLMQLARQISRLESGQ"
     gene            16759..19833
                     /gene="lacZ"
                     /locus_tag="ACHOQV_002996"
     CDS             16759..19833
                     /gene="lacZ"
                     /locus_tag="ACHOQV_002996"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFP9631337.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSKEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDISLLHK
                     PTTQISDFHVTTRFNDDFSRAVLEAEVQVYGELRDELRVTVSLWQGETQVASGTTPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVLRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLTENLSVTLPSAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQTEVALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
BASE COUNT         4668 a         5455 c         5186 g         4558 t
ORIGIN      
        1 acacaaacac ttaacatatg aactacataa agattataca gtcttatccc cgtacctggc
       61 agccccttgt ttttcagcta tcagctattt ttaacactta aaaattagca tctgtctgcg
      121 tcagaggtta cgtctctgcg ggcgtcgttg tgcttgtagt ataataaata aaatggagtg
      181 tcataaagtg gataaggttt tagattcagc gatcctttct tcggcaaata aaagaaaggg
      241 tatccttgcc attggcgcgc atcctgacga tatagaatta ggctgtggcg catcgctagc
      301 tcgccttgcg caaaaaggaa tttatattgc taccgtggtg atgactaccg gtaattctgg
      361 cgtagatgga ataatcgatc gccacgaaga atcacgcaac gctctaaaga tattagggtg
      421 ccaccaaact attcatctta attttgctga cacccgcgct catttacagc tcaatgatat
      481 gatttctgcc ctggaaaata tcataaaaaa tcaaattccc tctgatattg aaattatacg
      541 tgtatatacc atgcatgatg ctgaccgcca tcaggatcat cttgcagtct atcaagcctc
      601 aatggttgcc tgtcgcgcta ttccgcaaat tctcggctat gaaacaccaa gtacctggct
      661 ttcatttatg cctcaggttt ttgaatccgt gaaagaagaa tatttttctc ttaaactgac
      721 tgcgttaaaa aaacataaaa gtcagagcca gcgagattat atgcgcccgg agcgtctgcg
      781 tgcagttgct caatttcgcg ggcaacaggt caatagcgat ctgggtgaag gttttgttat
      841 tcataaaatg attctttgag caaattgcat gaaaacctgg atctttatat gtatggccgt
      901 agcaatattg ctatggtttc tgagtacgtt aaggcgtaag cccagccaaa agaaaggctg
      961 tattgacgcc attatacctg cttataacga aggcccgtgt ctggcgcagt cactggataa
     1021 tctgctgcgt aacccttatt tctgccgggt aatttgcgtt aacgacggct ccacggacaa
     1081 taccgaagcg gtcatggcgg aagtcaaacg caaatggggc gaccgcttta ttgccgtcac
     1141 gcaaaaaaat accggtaaag gtggtgcgct aatgaatggc ctcaattacg ccacctgcga
     1201 tcaggttttt ttaagtgatg ccgacaccta tgttccgccc gatcaagacg gaatgggcta
     1261 tatgctggca gaaatagagc gcggtgctga tgccgtaggc ggcattccct ctactgcgtt
     1321 gaaaggcgcg ggtctgttac cgcacatccg cgcgaccgta aagttgccga tgattgttat
     1381 gaagcgcacg ctacagcagc tcctgggcgg cgcaccgttt attatcagcg gtgcctgcgg
     1441 gatgttccgt actgatgtat tgcgtaagtt cggtttctct gatcgtacta aagtcgaaga
     1501 ccttgatctc acctggacat tggtggcaaa cggctatcgt attcggcagg cgaatcgctg
     1561 catcgtatac ccacaggaat gcaacagccc gcgtgaggaa tggcgtcgct ggcggcgttg
     1621 gattgtgggc tacgcggtct gtatgcgcct gcataaaaga cttttattta gccgcttcgg
     1681 tatcttcagt atatttccta tgctgttggt tgtgctttat ggcgttggga tttatctcac
     1741 tacctggttt aatgaattca tcaccaccgg gccacatggt gtggtgttgg caatgttccc
     1801 gcttatctgg gtcggcgtag tttgtgttat tggtgcattt agcgcctggt ttcatcgttg
     1861 ctggttgttg gtgcctttag caccgctttc cgttgtgtat gtattattag cttatgccat
     1921 ctggattatt tatggactta ttgccttttt tactggacgc gaacctcagc gcgacaaacc
     1981 cacccgctat tccgcactgg tggaagcgtc aaccgcttat tcccaccctt ctgtcacagg
     2041 aactgaaaaa ctatctgaag cttaatgtta aagagaaaaa tattcagatt gcagaccagg
     2101 tgattattga tgaaagtgcc ggtgaggttg ttatcggcgc gaatacccgt atttgccacg
     2161 gtgccgttat tcagggtccg gtagtgattg gcgcaaactg cctgataggt aattatgcgt
     2221 ttattcgtcc tggcacaata atcagcaatg gcgtaaaaat tggttttgcc accgaaatta
     2281 aaaatgcggt tattgaagcg gaagcaacga ttggtccgca atgctttatt gccgactcgg
     2341 tagttgcaaa ccaggcatat ttgggcgcac aagtacgaac cagtaatcat cgtctggatg
     2401 aacagcccgt gtctgttcga actccagagg gaattatcgc taccggatgc gataaattag
     2461 gttgttatat cgggcagcgt tcacgccttg gcgtacaagt tattattttg cctgggcgaa
     2521 ttatttcccc gaacacgcaa cttggcccgc gcgtgattgt agaacgtaat ttacctaccg
     2581 gaacttattc actccgacaa gaacttatcc gtacaggaga ttaaaatgat aaaacggacg
     2641 ttattagcgg cggcaatatt tagcacattg cccgcttatg ccgggttaac ttccattacc
     2701 gcgggctacg attttaccga ttattctggc gatcatggca accgtaattt agcgtatgct
     2761 gaactggtgg cgaaagttga aaacgcaacg ctgcttttta atctttccca ggggcgtcgt
     2821 gattatgaaa ctgaacattt caatgccact cgcggtcagg gtgcggtctg gtataaatgg
     2881 aataactggc tgacaacccg aacgggtatt gcctttgcgg ataatacgcc ggtctttgcc
     2941 cgccaggatt ttcgtcagga tattaacctg gccctgttgc caaaaacgct tttcacgacc
     3001 ggttatcgct acactaaata ttacgatgat gtcgaagtcg atgcctggca aggcggcgta
     3061 tccctctata caggcccggt gatcaccagc tatcgctata cccattatga ctccagcgat
     3121 gcaggtggta gttacagcaa tatgatttcc gtgcgtctga atgacccgcg cggcactggt
     3181 tatacgcaac tatggctaag ccgcggaaca ggtgcttaca cctatgactg gacgccagaa
     3241 acacgctacg gcagcatgaa gagcgtcagt ttgcaacgta ttcaaccgct gactgagcaa
     3301 cttaatctcg ggctgacggc aggtaaagtg tggtacgaca ccccaaccga tgattataac
     3361 ggtctgcaac ttgcagccca tctgacctgg aaattctgat tccttctgcc gcccgctatc
     3421 cggggcggcc ttccctgtcg attaggcccc tctttacttt ttgttatccg actacattca
     3481 ccggataaat tttattctcc agtgttatat actatagagg ggtatgcatt gacatataac
     3541 atacccccct atagtatatt gcgtgcagat gatgaggtgc gaaatgccca gtactccgga
     3601 agagaagaaa aaggtcctta ctcgagttcg tcgtattcgg gggcagattg atgctctgga
     3661 acggtcgctg gagggtgatg ccgaatgccg tgccatactc caacagatcg ctgccgttcg
     3721 gggcgcggct aacggactga tggcagaagt gcttgaaagc catatccggg aaacgtttga
     3781 ccgaaatgac tgctacagcc gcgaagtcag ccaatccgtt gacgacacta ttgaactggt
     3841 tcgtgcctat cttaaatagc tgaatctatt accatattga ggaagagcga gagatgaaat
     3901 cacgtgctgc cgttgcattt gctcccggta aaccgctgga aatcgttgaa attgacgttg
     3961 caccaccgaa aaaaggtgaa gtgctgatta aagtcaccca taccggtgtt tgccataccg
     4021 acgcatttac cctctccggc gatgacccgg aaggtgtctt cccggtggtt ctcggtcacg
     4081 aaggggccgg ggttgtagtt gaagtcggtg aaggcgtaac cagcgtcaaa cctggcgacc
     4141 atgtgatccc gctttacacc gcagagtgcg gcgagtgtga gttctgtcgt tctggcaaaa
     4201 ccaacctgtg tgtcgcggtt cgcgaaacgc agggcaaagg cttgatgcca gacggcacca
     4261 cccgtttttc ttacaacggg cagccgcttt atcactacat gggatgctca acattcagtg
     4321 aatacaccgt ggtcgcggaa gtgtctctgg ccaaaattaa tccagaagca aaccatgaac
     4381 acgtctgcct gctgggctgt ggcgtgacca ccggtattgg cgcggtacac aacacagcta
     4441 aagtccagcc aggtgattct gttgccgtgt ttggtcttgg cgcgattggt ctggcagtgg
     4501 ttcagggcgc gcgtcaggcg aaagcgggac ggattatcgc tatcgatacc aacccgaaga
     4561 aattcgatct ggcgcgtcgc ttcggtgcca ccgactgcat taacccgaat gactacgaca
     4621 aaccgattaa agatgtcctg ctggatatca acaaatgggg tatcgaccat acctttgaat
     4681 gcatcggtaa cgtcaacgtg atgcgtgcgg cgctggaaag tgcgcaccgc ggctggggtc
     4741 agtcggtgat catcggggta gctggttctg gtcaggaaat ctccacccgt ccattccagt
     4801 tggtcaccgg tcgcgtatgg aaaggttccg cgtttggcgg cgtgaaaggt cgttcccagt
     4861 taccgggtat ggttgaagat gcgatgaaag gcgatatcga tctggaaccg tttgtcacgc
     4921 ataccatgag cctggatgaa attaatgacg cctttgacct gatgcatgaa ggcaaatcca
     4981 ttcgaaccgt aattcgttac tgatttcccg caggtttacc cgtccagttc agacggggtt
     5041 cttaatactc tccccgggca gccgtccggg ggattaaccg tgagataatg actgatggaa
     5101 ctcattgaaa aacatgccag ctttggcggc tggcaaaatg tgtatcggca ttattcccca
     5161 tcactgaaat gtgaaatgaa tgtcggcgtc tatctcccgc caaaagccgc aaatgaaaaa
     5221 ttgccggtgt tgtactggct ttcaggcctg acctgcaacg agcagaattt cattactaaa
     5281 tcggggatgc agcgttacgc ggctgagcac aacattattg ttgttgcgcc agacaccagt
     5341 ccgcgaggca gtcatgtcgc agatgccgac cgttacgatc tcgggcaagg tgccgggttt
     5401 tacctgaacg caacgcaagc gccgtggaat gaacattaca aaatgtatga ctatatccgc
     5461 aacgagctgc cggatttagt gatgcaacat tttccggcaa cgaccagaaa gtctatctca
     5521 ggtcattcta tgggcgggct gggcgcgctg gtgctggcgt tacgtaaccc agatgaatat
     5581 gtcagcgtct cggcgtttgc gcccattgtc tccccatcgc aagtaccgtg gggacagcaa
     5641 gcctttaccg catatcttgg tgaaaataaa gatgcctggt tggattacga tccggtgagt
     5701 cttatttcac aaggtcaacg cgttgcggaa ataatggttg atcaggggtt gagtgatgat
     5761 ttttacgcag aacagctgcg gactccaaat cttgaaaaga tctgccagga gatgaatatc
     5821 aaaacgttaa tccgttatca cgagggttat gatcacagct attattttgt ctccagtttt
     5881 attggcgagc atattgcgta ccacgccaat aaactgaata tgcgttgagc atccgcgcac
     5941 gcatgccgga tacggcgtaa acgccttatc cggcctacat ttcacccgta agccgtaggc
     6001 ctgataagac gcgaaaagcg tcgcatcagg catctgcgca cggctgccgg atgcggcgtg
     6061 aacgccttat ccggcctaca cttcgcccgt aaaccgtagg cctgataaga cgcgtaagcg
     6121 tcgcatcagg catcactgca cggcttatct atgtttacca catcaaataa tcaggcactt
     6181 tgaagtcggc atacggatcg tcttcatcct gctcttcggc gctgagcgcg ctgtgcaaca
     6241 caatactgct ggcatcacgc tgagcaattt tatcggcaac actggcggga ataatggcgt
     6301 attcaccttc gctattgtta tcgaccaaca agcgggcaat cgccagacga ccattaatca
     6361 actgcgcctg tgtgagctta tcgacaaaaa tcttcttaat cagattgccg tcagtgaagt
     6421 taaaaccaat atcgccattg gcaatggtga ttcggttcat ttcaatgagc tgcttcacct
     6481 gggctttata ttctttcgcc aacgccgctt gtttttgctg ttcgctaagc tgtttatcac
     6541 gctcaagctg tgccttttta ttttcttcta ccgccgcccg agcttcacgc gcctgaacgc
     6601 gcgatttttt cgccgttctc tcgaccttcg ccgctttttt gctggtgact aatcctgctt
     6661 tgagcaactg ctcttgtaag gtaagttttg ccatctttgt ttccaaaccc gctgaataat
     6721 ttttagcgag tatacctgta aacgccgtag gtgtattggg ggcaagtccc tttcgcccct
     6781 gcttaataag gcgcgtcagg catcggcgca cggctgcatt cgtgatctcc ggctcatcaa
     6841 aataaatact gccaggccag caacaagaat acccggcgca gaagcggcca ttacgccaac
     6901 cgtgccaatg cctaatgcca gcattttccc ggccagtaac ggaccgctca tagcccccag
     6961 acgccctacc gccacggctg ttcccacacc tgttgcgcgg atctgcgaac tgtaaaacaa
     7021 cggtgccagg gcatacaaaa cgctttgccc acctgtcgca aacaaccccg cgacgaatcc
     7081 cgccagcaac ataccgttaa atgacgacac cgttcccagc gccagcagcg aagctaacat
     7141 gccgctataa atcagtagcg acatggttac tggacgcagc ttatccatca atgcgcccaa
     7201 cattaacgtc ccgcttgccg cccccatttg cagtgcaaac atcacccctg ccgcctgcga
     7261 tggctggaat ccttgctcca ccaaaagcag cggtagccag ttgatcaaca tgtagaccac
     7321 cagcagagtg aagaaataac acaaccacag cagcagcgtc gcggttgccg tttctggcgc
     7381 aaataacgca cgcagtggtg gcgcagcctg tttttcgcca gcgaacaccg ccgactccgg
     7441 cagccagcgc attaatagcg gcaccagaat caacggcacc acaccaccta cccaaaacac
     7501 cgtttgccat gctaagtttg cccccgcgaa acccagtgtc gccgccagcg ccgcgccaat
     7561 ggggacaccg caatacatca ggctcactgc cgtcccacga aaacgtggac ccgcggcttc
     7621 agacgtcagg gcgatcagat tcggcaacgc cgcccccagc ccgacaccgg tcatcagccg
     7681 cgcaaagacc agtgagggga aatcccaggc aatcgccgtt gccagtgaga acaaaccaaa
     7741 cagcgcaact gagccaatca aaatgcgctt gcgaccataa cggtccgcca gcattccgcc
     7801 aaccaacgcg ccgggtagca aaccgagtat tccggcgctg aatatccagc ccatttgcat
     7861 tttatcgagt gcgaaagcct gggcgatgcc acccgccgca atgccagccg cctgaagatc
     7921 cagtccttcc atcagagcga ccaaaaaaca aagcccgatg gtcagcatca ggcgggttga
     7981 agatgatgaa ggggtacgag tcgacataag atgaacctcg ttgcgagtgc aggtgaatcg
     8041 cggctgatac attgtcagcc gcgtaatcag aaccatttaa tgactgccta cgggcgaaat
     8101 ataggccgga taaggcgttt acgccgcatc cggcagtcgt gctgggatgg gcatacaaaa
     8161 tgcacgttct cggcagtcat cattatttgt tgttgcgcag atccagcgcc acgtcgacga
     8221 tcatatcctc ctggccgcca accatccggc gtttgcccag ctcaacgaga atatccaccg
     8281 cgcttaagcc ataacgcgcc gccgccgttt cacagtgacg caggaagctc gagtaaacac
     8341 cagcgtatcc cagcgccagc gtttcgcgat cgactcgtac cggtcgatcc tgcaacggac
     8401 gcaccaggtc gtcagcggca tccattaacg catagagatc ggtcccatgc tgccaaccca
     8461 gtttatccgc ggcggcaata aaaacttcca gcggcgcgtt acctgcgccc gcgcccattc
     8521 ccgcgaggct ggcgtcgatt cggtcgcagc cctcttccac cgccgcgata gagttcgcca
     8581 cgccaagact caggttatgg tgagcgtgta tgccggtttg cgtttctggt ttcagcactg
     8641 ctttcagagc gcggaaacgg tcacggatat cgctcatgtt catcgcaccg ccagaatcca
     8701 ccacataaat acaggtcgca ccgtagcctt ccatcagctt tgcctgcttt gcgagattct
     8761 ccggcgtggt catatggctc atcatcagaa aaccaacggt gtccattccg agctcgcggg
     8821 catactgaat atgctgggcg gaaacatcag cttcggtaca gtgcgtagcc acacgaacca
     8881 cccgcgcgcc agcctgccag gcatttttca gatcgtgaat agtgccgatt ccaggcagca
     8941 acaacgtcgc gattttggcg tgcttcacca catccgccgc cgcttcaatc cattcaaggt
     9001 cgctatgtgc gccgaaacca tagttaaagc tggaaccttg caaaccgtcg ccgtgggcca
     9061 cttcaatcga atccacgcgg gcatcgtcca gtgctttggc aacctggcga acgttttcca
     9121 atgaatactg atgacgaatg gcgtgcatac cgtcacgcaa cgtgacgtcc gagatataaa
     9181 gttttttacc gttcatgccg cttctcctgc cttgcgcgcc agtgactggg ccattttttc
     9241 cgctgtcgcc agcgcactgg aagtcataat gtcgaggttg cctgcatagg caggcagata
     9301 atgcgctgcg ccttcgactt ccagccagac cgccgttttc agtccggaga actgccccac
     9361 gcccggcaaa ttaaccggtt tatcctgcgg gataacttca aactgcacgc gctgtttcag
     9421 gcgataaccc ggtacgtaag cctgcaccgc ctcagccatt tcattgattg aggcttcgat
     9481 atcatcttgt gaagcttcgt cgctcaatac atacaccgtg tcacgcatca tcaacggtgg
     9541 ctctgctggg ttaagcacaa taatcgcctt cccttttgcc gcgccaccca ccacttcaat
     9601 ggcgcgcgaa gtggtttccg taaattcatc gatattggca cgcgtgccag gtccggcaga
     9661 tttactggca atagaagcga taatttcggc gtaatgaaca cgcgccacgc gtgaaactgc
     9721 cgccaccatt ggaatggtgg cctggccgcc gcaggtgacc atgttgacgt tcagttgagc
     9781 gacgttctcc tcgaggttaa cgaccggcac gcagtagggg ccgatggcag caggcgtcag
     9841 gtcaattaag cgaatatccg gtttcgcttc gcgcaaagcg gcatcgtttt tcacatgcgc
     9901 acccgcgctg gtcgcatcaa atacaatgtc gatatcagca aattcaggca tgttcatcag
     9961 gccgatcacc ccttcatggg tggtggcgac gcccatacgt ctggcacgcg ccagaccgtc
    10021 ggactgagga tcaatgccga ccatcaccgc catctccaga tgctggccgt gacgcaaaat
    10081 tttaatcatc agatcggtgc caatgttgcc tgaaccgata atggcgactt tacgcttact
    10141 catgacagac ttccttttgg ggctgcgctt gaaaatgtcg cagcaactga acctatgcct
    10201 tcaatatggg cttcaaaacg atcgcccgca ttcaccgcca ccatcggacc taatgccccg
    10261 gtaagaatga tatctccggc gcgcagcggt tcacccaggc tggccatttt gcgtgccagc
    10321 cagacggcgg cattaagcgg atgtcccagg cattcgctgc cgcgcccgct ggaaacctct
    10381 tcgttattac gcgtcatctt catggcgcag tttttcaggt ctaaccccgc cggacgttgc
    10441 gccggaccgc cgatgacata caccccgcag gaagcgttat ctgccacggt atcgacaaac
    10501 tgaatcgacc agtcgcgaat gcggctcccc accacttcca gcgccggaag tacccattca
    10561 atggcgttat acaattcgtc gaaggtgata tcggttgcgg gtagatcgcg gttcaacacc
    10621 agtgcaatct ccgcttcaat gcggggttgc agaacacggg aaaaaggaat ggtttcgtta
    10681 tcgccataac acatgtcggc aaataatgtc ccgaaatccg gttgatcaac gcccagttgt
    10741 tgctgcactt tcggatgcgt cagccccact ttgcgcccca ccactcgccg tccctgagcg
    10801 acgtcgtgtt gcacattgat gtgctgaatg gcgtaggccg cttcagcgtt atcgatacca
    10861 atcagatcgc gcagcggcgc aatcgcttcg ccctgctctg cggcgcgacg taaatccgcc
    10921 gccagttgct caagagtatg cttcgtcatg accgttcctt aagggcgtgc gaggaaattc
    10981 agcaccagtt gattgaaagc gtcggcatgt tcccactgcg cccagtgacc acagtcgcgg
    11041 aagatatgca gttcagaacc ggcaatgccg gacagcagac gcagacccgc atccatcggc
    11101 acaaagcggt cgttgcgccc ccagacaatc agggtttgcg ctttgatttc cgccagacgt
    11161 gggccaaaat ccgggaactg tttcggattg gcttccaggc tcttaacgaa gttttccagg
    11221 tgatcgcggc gcgacagcat attattcagg cgcgcttcaa acagggcgtc ggtcaaatcg
    11281 ctggtatcaa aaacgaagat atccatcatc agcttcaggt tttcgatagt cggctgacga
    11341 taaagctgat tcagtcgctt aataccttcg gttggcatcg gcgtaaacaa actcatgccg
    11401 cccgtaccgc cgcccatcag caccagtttg ccgacgcgct ccggccagtt aagggtgaac
    11461 gccacagaac tgtggccgcc catcgagttg cccagcaggt ggattttggc gatatccagt
    11521 tgatccacca cgcttttcag gattcgcgca ttaagatccg atcgtgaacc actattaacg
    11581 atcgaatcgc tcttacccca acccggacaa tccagcagga tcacccgata gcccgccttt
    11641 accagcggat cgatattgcg gctgaagttc gcccagccag tagcacccgg gccggaacca
    11701 tgcagcagga caacagtttc gtcgccttgt ccgcagtcat taaaatggat gcgcagcgtt
    11761 ttacccgctt cttctacatt cagaaaacgg ctggtggcgg cttcggtttg tggctgataa
    11821 ctcatcatct tctcctgcat attcagttct ctgttctggc gcttaacgag ccaaatccgg
    11881 caatccactc cgggattggg cggtaataac gcccttcgct acgccagttg ccaaacgcag
    11941 aaatagcggc aaaagcggcg acccaggttt tgatttcatg tgtcgacttt ccggcaatgg
    12001 cggaaagctc ttcgttactg acggcatcca gttcctgtat gcgtccctgc tccagcaaag
    12061 tcatgaactg gttatcccaa atcgggttga gcggatgcag cgttctctga tcctcaacaa
    12121 acttctcagc ggcgctaatc acccgttgct gacgcaattc gcgctcactg gcgggtaaat
    12181 ctttcccgct ccccaacaga cggtcgcgca tatgggcatc ggctttcgcc agttctggca
    12241 ccggcggctg atgggaaagc ccaccggaac ccaggaacag cacgcgttta ttgagagtac
    12301 tggtgaaacg tccaatggct tcccccaaca tgcgggtacg ctggaaaccg ggcaacggcg
    12361 tggcgacacc gttgatgaac acaggcagaa ccggcacctt atccagccca ccgagcagga
    12421 actccagcgg ctgggcgaac ccgtggtcca cctgcataca gtaagaaacg gcaagatcga
    12481 ttccgctctt catgacggca tgcgcacagg cctccgccag ctcaacaggc acgggcagct
    12541 ctcctgccgc actaccgaaa tcaccaattg ccgtcgctcc aacgcctaaa cagaacggtg
    12601 gcatcacgtc atagaaaaag ccgttgtagt gatctggcgc aaacagcacc accaactcag
    12661 gggagaaagc cgcaatacgc tcgcgggcgc tggcaatcac gccattgacc tcatcgagca
    12721 cctcttgcgc cgggtcgaca tatcccacca gcggcgagtg ggaaagacag tgaagataag
    12781 cgtgcatatc aggctacctt ttcgacagaa acgtcggcat cagggcgggt cagcgtcatt
    12841 accgacgcca gtttattcag cgtattaccc agagtttgcg gaatggcggt ggcggcaaca
    12901 aagcgatccg ggcgcatcac caccagcgaa gcattgtgtt gcgcgaacca gctacgcagg
    12961 cgaccttgcg tatcgcccac gcgtagtacg ccgtcgtggt tatcctgtgc ggtatgaatt
    13021 tgcacttccg gcaccacctg aatgaagcgt gtgcccaacg cgcgccactg ctggatttgc
    13081 tcgtcgctca tcccccacag tggattgcat ccccagccaa ttaccgcgaa gttcgcgccg
    13141 atcgcgttat cgagcagcgt cacgtcgccg ttttccagcg tgactttcgg ctgaataaac
    13201 atcttgccga ccggagagtg cttcgcttcg ccctcacgca ccagcgcacc gccgtaatat
    13261 tgcggcatcg gcttgaagcg catttcgagg aagtagcgtt ttactggcgg cagataattc
    13321 aacagccagg aaacgccgtc acgtaacgta ccctgccagc gtttcggcgg agccagcacg
    13381 ttgcccgccg tcacggacag gtcaatcatc gctttggcgt gatcgcgacg ttcttgttga
    13441 taggtatcga gcagcgcatc gcgggctttc ccctggataa ccaacgccag tttccatgcg
    13501 aggttaaagg cgtcgcgcat accactgtta tagccctgcc cctgccacac cggcatgatg
    13561 tgcgcggcat cgcccgccag cagtacgcga tcaatacgaa aacgttgcgc cagtcgcgcg
    13621 ttgtgggtgt agacacgctg gcgaatcaat tcaacattgt ccggattagg cagcactttg
    13681 cttaacagct tgcgcatatt ttgcggctca cgcagctgtt cttcggtttc tcccggcatc
    13741 accataaatt caaagcgacg taccgcatga ggcagcgcgg cagaaacata cgggcgcacc
    13801 ggatcacaac acaaatagat atgcggcgta cttaacggat cgttggcgat atctaccaca
    13861 atccactgat ttggcgcagt tttaccttca aacggcacat tcagggtgcg acggacaaaa
    13921 cttgccccac catcacaggc caccagccat tgggctttga ccgtttcccg ctgcccttct
    13981 gccgttttca ggtgcaaggt cacttcgtca tcttgctggc tgaaggcctc cagctcgcgg
    14041 gaaaacaggc agcgcacatt cggaaaacgc gacaatcctt ccagcatcac cgcatcgacc
    14101 tgaggctgaa taaaggcgtt acggcgcggc cagccaaatt catcggtcat tggctgaata
    14161 tcagcaaaac agcgaccttt tggggtgaga aaacgcatcg cgtgccacgg cgtagtgtgc
    14221 ggcagaacat tatcgaccag gccgaccgac tgcatggtgc gcagcgcctc gtcatcaata
    14281 ccaatcgcac gcgggtagtc gatcaactta tcgagtttct ccaccaccag cacgtcaatg
    14341 cccatctggc cgagatagtt cgccatcatc agcccgaccg gaccggcacc agcgatcgcc
    14401 acctgaacgc tatggttaac agcaggctgg atgtcaggat gttgtattgc catttcagta
    14461 cctcacgact cggacaaaat gccgttgcgc gcacagtacg gcgcaactta ttttgttaaa
    14521 aacatgtaaa tgatttttta ttgtgcgctc agtataggaa gggtgttttc ggctacaatc
    14581 aaaacatgcc cgaatgtgca ccaagtgcac aacgttgttt taactataga aatgtcaatt
    14641 aatatgcaga acaatgagca gacggcatac aaaaccgtgc gcggcttaac ccgcggtcta
    14701 atgttattaa atatgttaaa taaacttgat ggcggtgcca gcgtcgggct gctggcggaa
    14761 ctcagcggcc tgcatcgcac cactgtgcgg cgactgctgg agacgctgca ggaagaggga
    14821 tatgtccggc gtagtccctc cgatgatagt ttccgtttga ccatcaaagt gcggcaatta
    14881 agcgaaggat ttcgtgacga acagtggatt tctgcactgg cggccccact gctgggcgat
    14941 ctgttgcgcg aagtggtatg gccgacagat gtgtccaccc tggatgttga tgcaatggtg
    15001 gtacgcgaaa ctacccatcg tttcagccgc ttatcttttc accgggcaat ggtcgggcga
    15061 cggttaccgc ttctgaaaac cgcctcgggc ctgacctggc tggccttttg cccggaacaa
    15121 gaacgcaagg aattaatcga gatgttagcc tcccgccccg gtgatgacta tcaactggca
    15181 cgggaaccgt taaagctgga agccattctg gcgcgcgcgc gcaaagaggg gtacggacag
    15241 aactaccgcg gctgggatca agaagaaaag atcgcctcta ttgccgtacc gctgcgcagt
    15301 gaacaacggg tgattggctg tctgaatctg gtgtatatgg cgagcgcaat gaccattgaa
    15361 caggcagcgg aaaagcatct tccggcgcta caacgggtgg caaaacagat cgaagaaggg
    15421 gttgaatcgc aggctattct ggtggccgga cggcgaagcg gcgtgcattt acgttgacac
    15481 catcgaatgg cgcaaaacct ttcgtggtat ggcatgatag cgcccggaag agagtcaatt
    15541 cagggtggtg aatgtgaaac cagtaacgct atacgatgtc gcagagtatg ccggtgtctc
    15601 ttatcagacc gtttcccgcg tggtgaacca ggcctgccac gtttctgcga aaacgcggga
    15661 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
    15721 ggcgggcaag cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
    15781 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
    15841 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
    15901 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
    15961 ggaagctgcc tgcgctaatg ttccggcttt atttcttgat gtctctgacc agacacccat
    16021 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
    16081 attgggtcac cagcaaatcg cgctgttagc aggtccatta agttctgtct cggcgcgtct
    16141 gcgtctggcg ggctggcata aatatctcac acgcaatcaa attcagccga tagcggaacg
    16201 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
    16261 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
    16321 cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac
    16381 cgaagacagc tcgtgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
    16441 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
    16501 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc cttccgccca atacgcaaac
    16561 cgcttctccc caggcgttgg cagattcttt aatgcagctg gcgcgacaaa tttcccgact
    16621 cgaaagcggg cagtgagcgc aacgcaatta atgtgagtca gctcactcat taggcacccc
    16681 aggctttaca ctttatgcgt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    16741 ttcacacagg atacagctat gactatgatt acggattcac tggccgtcgt attacaacgt
    16801 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcggcaca tccccctttc
    16861 gccagctggc gtaatagcaa agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    16921 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
    16981 ctggagtgcg atcttcctga cgccgatact gtcgtcgtcc cctcaaactg gcagatgcac
    17041 ggttacgacg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt
    17101 gttcccgcgg agaatccgac aggttgttac tcgctcacat ttaatgttga tgaaagctgg
    17161 ctacaggaag gccagacgcg aattattttt gatggcgtta attcggcgtt tcatctgtgg
    17221 tgcaacgggc gctgggtcgg ttacggccag gacagccgtt tgccgtctga atttgacctg
    17281 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtactgcg ctggagtgac
    17341 ggcagttatc tggaagatca ggatatgtgg cgaatgagcg gcattttccg tgacatctcg
    17401 ttgctgcaca aaccgaccac acaaatcagc gatttccatg ttaccactcg ctttaatgat
    17461 gatttcagcc gcgcggtact ggaggcagaa gttcaggtgt acggcgagct gcgcgatgag
    17521 ctgcgggtga cggtttcttt gtggcagggt gaaacgcagg tcgccagcgg caccacgcct
    17581 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac gctacgtctg
    17641 aacgtcgaaa acccggcgct gtggagcgcc gaaatcccga atatctatcg tgcggtggtt
    17701 gaactgcaca ctgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc
    17761 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgcggc
    17821 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg
    17881 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg
    17941 aaccatccgc tgtggtacac cctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc
    18001 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggtta
    18061 ccggccatga gcgaacgcgt aacgcgaatg gtgctgcgcg atcgtaatca cccgagtgtg
    18121 atcatctggt cgctggggaa tgaatcgggc cacggcgcta atcacgacgc gctgtatcgc
    18181 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc
    18241 tccgcaaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gccctttccg
    18301 gctgtgccga aatggtccat caaaaaatgg ctttcgctgc ctggagaact gcgcccgctg
    18361 atcctttgcg aatatgccca cgcgatgggt aacagtcttg gcggcttcgc taaatactgg
    18421 caggcatttc gtcagtaccc ccgtttacag ggcggcttcg tctgggactg ggtggatcag
    18481 tcgctgatta aatatgatga aaacggcaac ccgtggtcag cttacggcgg tgattttggc
    18541 gatacgccaa acgatcgcca gttctgcatg aacggtctgg tctttgccga ccgcacgccg
    18601 catccggcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg
    18661 cgaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac
    18721 tggtcggtgg cgctggatgg caagccgctg gcaagcggtg aaatgcctct ggatgttgct
    18781 ccacaagata aacagttgat tgaactgcct gaactaccgc agccggagag cgccggacaa
    18841 ctctggctta cggtacacgt agtgcaaccg aacgcgaccg catggtcaga agccggacac
    18901 atcagcgcct ggcagcaatg gcgtctgacg gaaaacctca gcgtgacact cccctccgcg
    18961 ccccacgcca tcccgcaact gaccaccagc gaaacggatt tttgcatcga gctgggtaat
    19021 aaacgttggc aatttaaccg ccaatcaggc tttctttcac agatgtggat tggcgatgaa
    19081 aaacaactgc tgacgccgct gcgcgatcag ttcacccgcg caccgctgga taacgacatt
    19141 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tagaacgctg gaaggcggcg
    19201 ggccattacc agaccgaagt ggcgttgttg cagtgcacgg cagatacact tgccgacgcg
    19261 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg
    19321 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtagcg
    19381 agcgatacac cgcatccggc acggattggc ctgacctgcc agctggcgca ggtcgcagag
    19441 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc
    19501 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc
    19561 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc
    19621 gacttccagt tcaatatcag tcgctacagc caacaacaac tgatggaaac cagccatcgc
    19681 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt
    19741 ggtggcgacg actcctggag cccgtcagtg tcggcggaat tccagctgag cgccggtcgc
    19801 taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc
    19861 gtatttc
//
LOCUS       DAZKGF010000090        19782 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZKGF010000090 DAZKGF010000000
VERSION     DAZKGF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.090"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            184..684
                     /gene="tssB"
                     /locus_tag="ACHOQV_002997"
     CDS             184..684
                     /gene="tssB"
                     /locus_tag="ACHOQV_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP9631338.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSGK"
     gene            719..925
                     /locus_tag="ACHOQV_002998"
     CDS             719..925
                     /locus_tag="ACHOQV_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631339.1"
                     /translation="MAAHKNISGKHPLSDEDILQAVYRVCFSPSGEINRDGVDALITT
                     LDRRISDLLDEIIHLCEFQQSAPY"
     gene            994..2469
                     /gene="tssC"
                     /locus_tag="ACHOQV_002999"
     CDS             994..2469
                     /gene="tssC"
                     /locus_tag="ACHOQV_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP9631340.1"
                     /translation="MSLQEEELVSSHAGQTDQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2476..2889
                     /gene="tssE"
                     /locus_tag="ACHOQV_003000"
     CDS             2476..2889
                     /gene="tssE"
                     /locus_tag="ACHOQV_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP9631341.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2893..4743
                     /gene="tssF"
                     /locus_tag="ACHOQV_003001"
     CDS             2893..4743
                     /gene="tssF"
                     /locus_tag="ACHOQV_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP9631342.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     LRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSHPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPEHYDVFSVNGVISQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERTGQHPLI"
     gene            4707..5789
                     /gene="tssG"
                     /locus_tag="ACHOQV_003002"
     CDS             4707..5789
                     /gene="tssG"
                     /locus_tag="ACHOQV_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP9631343.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSDVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5814..7094
                     /gene="tagH"
                     /locus_tag="ACHOQV_003003"
     CDS             5814..7094
                     /gene="tagH"
                     /locus_tag="ACHOQV_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFP9631344.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCVFTQQGNVGHGPECQWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSTDPLQALQSESLITAGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSVAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7091..7615
                     /gene="tssJ"
                     /locus_tag="ACHOQV_003004"
     CDS             7091..7615
                     /gene="tssJ"
                     /locus_tag="ACHOQV_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP9631345.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7618..8949
                     /gene="tssK"
                     /locus_tag="ACHOQV_003005"
     CDS             7618..8949
                     /gene="tssK"
                     /locus_tag="ACHOQV_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP9631346.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGILPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWAEIAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8954..9715
                     /gene="icmH"
                     /locus_tag="ACHOQV_003006"
     CDS             8954..9715
                     /gene="icmH"
                     /locus_tag="ACHOQV_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFP9631347.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FYGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9724..12507
                     /gene="tssH"
                     /locus_tag="ACHOQV_003007"
                     /gene_synonym="clpV"
     CDS             9724..12507
                     /gene="tssH"
                     /locus_tag="ACHOQV_003007"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP9631348.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEINLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQALVQEIIALRQQLLGVAEDD
                     AAPLPDADTVEDTPPESESESESESEQDNTGAVPADEADREQPEETAETVSPVQRLAQ
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQSFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEELYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIM
                     SRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDDAFTADVEDA
                     QNDESVTKDETVL"
     gene            12504..13247
                     /gene="vasI"
                     /locus_tag="ACHOQV_003008"
     CDS             12504..13247
                     /gene="vasI"
                     /locus_tag="ACHOQV_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFP9631349.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAETSSMAGVAVAVATTP
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRVACHWAGE"
     gene            13252..14664
                     /gene="tssA"
                     /locus_tag="ACHOQV_003009"
     CDS             13252..14664
                     /gene="tssA"
                     /locus_tag="ACHOQV_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP9631350.1"
                     /translation="MAMNLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQHDALSGELAKLAQCWQSYN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSRQTTATSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAT
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14773..18207
                     /gene="tssM"
                     /locus_tag="ACHOQV_003010"
     CDS             14773..18207
                     /gene="tssM"
                     /locus_tag="ACHOQV_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFP9631351.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGIYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     GLFPQALLAEPNLATESRTWLMRSRRRLTVFSTTGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPLGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKL
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18218..19628
                     /locus_tag="ACHOQV_003011"
                     /pseudo
     CDS             18218..19628
                     /locus_tag="ACHOQV_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19594..>19782
                     /gene="tssD"
                     /locus_tag="ACHOQV_003012"
     CDS             19594..>19782
                     /gene="tssD"
                     /locus_tag="ACHOQV_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFP9631352.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIKK"
BASE COUNT         4629 a         5452 c         5497 g         4204 t
ORIGIN      
        1 aatacccctc tttacgatcg ataagaagtc cttttcttat ttattttgaa gtgtagaatg
       61 cggggtgatg aaggagtggt aattttattt tcaccgccat tagggcggca ttgccgatat
      121 ttttttgcgg tacaaatatg ccgtgttcat ggcctgtacg agataacatg acggatcact
      181 tttatgagca aaaaatttga aggcagcgtc gcgcctcgcg agcgtataaa tatatcctat
      241 gtcccgaaga cggacggcca gacggctgaa gtagagcttc cgcttaacat gctcgttgtc
      301 ggcgataccg gaaatacgca ggagacatcc ccactggatg aacgtcaggc agtttccgtt
      361 aacaagcaca atttcggtgc agtaatggct gaagcagcca ttggcctcaa ttttaccgtc
      421 cccgccacgc tgaagggaag cacaacggac gatgaactga atgtggcgct gaatatcaag
      481 tcactggatg atttttcacc ggacagcgtg gcccggcagg ttccggaagt gaataaattg
      541 ctggaactgc gtgaagcact gactgcgctg aaggggccga tgggcaacct tccggcattc
      601 cgtacccagc tccaggcatt gctggaaaat gaggagtccc gcgagcagtt gcttaaagaa
      661 atcggccagg tgagtgggaa ataaccacca ctatttcatt aaacagggtt atccagacat
      721 ggcggcacac aaaaatatct ccgggaaaca ccccctctct gacgaagaca ttttgcaggc
      781 ggtttaccgg gtatgtttca gcccgtccgg tgaaatcaac cgggatggcg tggatgcgct
      841 gattacaaca ctggatcgcc gtatcagtga tctattggat gagattatcc atctttgtga
      901 gtttcagcag agcgcgccgt actgacggcg cgtacttcac tgacggcgaa aacccggttt
      961 cagaaacaca gctaaaacag aaggattttc agtatgtctt tacaggaaga ggaactcgtt
     1021 tccagtcatg ccgggcagac tgatcaggcg tcgtcgctgc ttgatcaaat catggcacag
     1081 acccgtattc agcctggttc ggaaggttat gacgttgccc gccagggtgt gacagccttt
     1141 attgccagca tcctgcaatc cactgcatct gccgaaccgg tcaataaact ggccgtggac
     1201 agcatgattg cagatataga tgaacgcatc agccgccaga tggatgtcat catccatgcg
     1261 cctgcctttc agcaggtcga gtcgttctgg cgctcactga aaaccatggt ggatcgcgtt
     1321 gatttccgtg aaaacatcaa ggtcaacgtg cttcatgtca cgaagcagga acttctggaa
     1381 gactttgaat ttgccccaga aattattcag tccggatttt ataaacacgt ttattcgtcc
     1441 ggattcggac agtttggtgg tgaacccatc gctgccgtgc tgggcgctta tgagttcaaa
     1501 aataccgctc ctgacatgaa actgctgcaa tacgtcagtg ccgtgggcgc aatggcgcac
     1561 gcaccgttcc tgtcctccgt ttccccggag tttatggggc tgaactcgtg gaccgaactg
     1621 cctaatatca aagatcttta tgccatcttt gaaggtccgg cctacaccaa atggcgcgcc
     1681 ctgcgtgact cggaagattc gcgctattta gggcttacgg caccccgctt cctgcttcgc
     1741 cagccctatt caccaacaga taaccctgtt aagaacttta actattacga agatgtcagc
     1801 cagaaccacg aagattatct gtggggtaat acggcctgga tgctggcatg caatatcgca
     1861 gacagttttg ccaaataccg ctggtgtcca aacattatcg gtccgcaaag cggcggcgca
     1921 gtgaaagatc tgccggtgca tctgttcgaa acgatggggc agattcaggc caaaatccca
     1981 accgaggtgc tggtcaccga tcgccgcgaa tttgaactgg ctgaagaggg ttttatcacc
     2041 cttaccatgc gtaaagactc tgataatgca gcctttttct ctgcaaactc ggtacaaaaa
     2101 ccgaagcact ttccgggaaa agacgcagaa accaactata agctgggtac gcagcttcct
     2161 tatctcttca tcatcaatcg gttagcgcac tacatcaagg tgttgcagcg tgaacaactg
     2221 gggtcatgga aagaaaggag cgacttagaa agagagctta atacctggat ccgtcagtat
     2281 gttgccgacc aggaaaaccc gcctgcagac gtgcgcagcc gcaaacctct gcgcgctgcc
     2341 aaagttgagg ttatggatgt agaaggcgaa cccggctggt accaggttgc gctaagcgtg
     2401 aggcctcatt ttaaatttat gggggcaaat tttgagcttt ccctggttgg ccggttagac
     2461 agggagtaac acaccatgat accggatcgg gaaagaacca caggttctct ttttgagcgt
     2521 atggaagcct cttcagccag aaaccggcag ggaggcagta ttcattcgct caggcaatcc
     2581 attcgccaga acctacgaaa cattcttaat acccggtccg gtagttgccg gggtgcgcct
     2641 gagctgggca ttgatgaacc ggaaggggct gaaaacttcc gcgaatcaat gagtcgggcc
     2701 atcgagcaat gtatagaacg ctatgaaccc cggatctcac acgctgaagt gcaggcagtg
     2761 gtttcatccg cctccagccc gctggatatg acgtttcata ttacagcctg ggtgacattt
     2821 aacgaaacgc atgaggtgct tgaatttgat atggcaccga atggcagcca gcattaccgg
     2881 gtggactgac aaatggaatt tgaagaacgc tatttccggg aagaactcga ttacctgcgc
     2941 cagcttagca agctgctggc aacggaaaaa ccccatctgg cccgcttcct ggccgaaaaa
     3001 gatgcagatc cggatattga acgcctgctg gaaggggtgg cttttcttac cggcaatctc
     3061 cgccagaaaa ttgaggatga attcccagaa ctgacgcacg ggcttattaa gatgctctgg
     3121 cctaattacc tgcgtccggt tccggcaatg acccttattg aatatacgcc ggatatggat
     3181 aagtcttctg taccggtgtt aatcccccgt aatgagcagt ttacaaccaa cgccggggaa
     3241 atcagagttg atgaagtgct gccctctgat gctaaaaagg aggagcctcc tccctgtacc
     3301 ttcaccctct gccgggatat ctggctgctg cccgttcgcc tggagcagat tgaaaaccgc
     3361 agtacgaccc gtaatggtgt tatcaacatc accttttcgg tcgcaccggg aacggacttc
     3421 cgcacgctgg atctgaacaa acttcgcttc tggctcggca atgacgacaa ctatacccgt
     3481 gaccagcttt atttatggtt ctgcgaatac ttgcagggtg ctgacctgac tgtgggtgaa
     3541 cagcatcttc gcctgcctga gtttatgcta aaagctgtcg gttttgagcc gcaggatgcc
     3601 atgctgccct ggccgaaaaa cgtccacagc ggctaccgga tccttcagga gtatttctgt
     3661 taccccgatg cgtttctctt ttttgatctt tgtggttgtc cggctttgcc tgacggattg
     3721 caggcggaat tctttaccct gcaactgcgt ttttcgcacc cattgcccgt ggacatccgg
     3781 ttgcgccgcg actccctgcg cctgtattgc gcgcctgcca ttaatttatt tatccaccat
     3841 gcagaagcca tcacgctgga caaccggcgg gcagactatc cgctggttcc cagccgccat
     3901 tatccggaac attacgatgt cttttccgtt aacggcgtga taagccaggt gcaggatatg
     3961 ttcaggaaaa aagatctggg tcgtcctgtt tcgacgcagg ccgcgcgcca gtggcctgcc
     4021 tttgaaagtt tcagccatca gatggaatac agccggaagc gggaagtggt gtactggcat
     4081 caccggacca aaacatccct gttccatcgc ggctttgatc atacccttgc ttttatacat
     4141 gctgatggca gttatccgtc agacgaatcg ctgctcagta atgaagtggt ttcggtatcg
     4201 ctgacctgta ccaaccgtga acttccgtca caaattcgtt ccggcgatat caccggcaca
     4261 accggtaaaa atgcggctgt tgcttcattt cgcaacatta cccgcccgac gcaaccgctc
     4321 tggccggtca ttgatggcag cctgcactgg tccctgctct ccgccatgaa cctgaattat
     4381 ctgtcattac tggatacgga cgcgctgaag caggtcatcg ccaactttga tcgccacgca
     4441 atccatcatc cgcagacggc gcggctgtca caacaaaagc tggatgccat tgagcgtctg
     4501 gagacccgcc ctgtcgatcg cctgtttacg ggcattcccg tccgggggct ggcctccacg
     4561 ctgtatctgc acccggagcc gtttgtctgt gaaggggaaa tgtatctgct cggtacggtg
     4621 ctttcgcatt ttctgtcgct gtacgccagc gttaactcat tccacatgct gaccgttgtg
     4681 aacacagaaa gccaggagac atggaaatgg acggaaagaa ccgggcagca tcctcttatc
     4741 tgagtccggg aaatcccccg gcagataaag agcagaatga tccgctggca caggtttttc
     4801 ataacgcctg ctcttacaat ttttttgcga tggcggagct gttgcaccgc ctggcaaagg
     4861 gtgaaaaggg gacgccagaa ttatccctgc gtgacgatcc ggcacaggaa accctgcgtt
     4921 tcagcgccga tgccagcctt gccttcccgt gcagtgatat cagtgcgctg aaacgggata
     4981 cgtcaggggc attcaggatg accaccacct ttatggggtt acaggggagc cagtcgcctc
     5041 tccccggtta ttacctcgat cacctggcct ggaaagcggt acatgaacaa agtccggtag
     5101 gtgattttct ggatatgttc agccaccgcc tgacccagtt tgtctggcat atctggcgta
     5161 agtaccgcta tcacatcagt tttcgtaacg gcggtgtgga tgcgttctcg cagcgcatgt
     5221 actcccttgt cgggctgggc caccgccagc ttcgcgataa gctggcgatt aatcacagca
     5281 aaatgctggc gtattccggg atcctggcaa atccgggacg atccccggaa ataatctgcg
     5341 gcctggtgtc ccactgcttt gatttgtcag acgtgacgct gcaaaactgg caacgtcgca
     5401 aggtggatat tgaaccggat cagcaaaaca gtctggggag ctattcactt aaaaatggcg
     5461 aaaaactggc gggaagatcg gtactgggca actttgttct gggtacacgg gtgcccgatc
     5521 tcagcggaaa attccagctc agtattacca gtctgacccg aaaacagttc ctctcttttc
     5581 tgccgtccgg cgaaaacttc ctgccactga cgatgtttgt gtccttcatc ctgcgcgatc
     5641 agcttgcctg ggacttacat ctggggctgg cgccggagca ggtgggcgca atgcgcctgg
     5701 gcgataacaa aagtgcgctg ctgggctgga ccagcttcct cggcacaccg gaagaacgac
     5761 cctcagtcac catcagggta cggtcgtaaa ttatgataat cacggagcag aatatgccgg
     5821 aagagaaact acagacgctt tcattgcagg tcattaacgg cagtgagctg gaaagcgggc
     5881 gggcggcgcg ctgtgtgttc acacagcagg gaaatgtcgg ccatggcccc gaatgccaat
     5941 ggtcggtaca ggatcgtcag cagagcattc cggctcaggc ttttaccgtt atcctgcacg
     6001 atggcacatt ttgtctgcgc ccgcagacgg cacaactgtg gctgaaccag gcaaaagtca
     6061 cagcaacatc agacctgata cagctgcgcc agggcgatga gatccagata gggcgtctga
     6121 tggtgagggt tcatctgaac cggggagata tcccccatta cgatgaggaa atggccactc
     6181 ccgaaaccat cgtcaccaat cgcgatatgc tcacggatac cctgctgtca acggagggtg
     6241 cgccacacta tccgggaatg actcaccggc atcagcttgc agacaccgtg gtaaacggtt
     6301 tttctaccga tccactccag gcacttcagt ccgaaagcct gattaccgcg ggcgatccgc
     6361 tttcaggcat tgcggctgtc cggccatcgg caccgctgtc cgatccggca agtaatgggg
     6421 ggatcaatac tccgtttatg gatctgccgc ccatttatgc cagccccggc gatcgtaatg
     6481 atgacgtctc tgtggcagaa atggcgcaac gccaccttgc ggtcaccccc ttactgcgcg
     6541 gtctcggcgg ttcgcttacc gtgagcaatt ccgacgatgc ggatgatttt ctggaagagg
     6601 ccggacgcac gttacaggcc gcaataaaag gtctgctcga tttgcagcaa cagcgtaaca
     6661 gcctctcaga caaacatttg cgcccgctgg aagataaccc gctgcgcctg aacatggatt
     6721 acgccaccgc gctcgacgtg atgtttgccg aaggtaaaag tccggtacat ctggcggctc
     6781 ccgccgctgt cagtgaaagc ctgcgcaatg tccgccacca cgaagaagct aaccgggcag
     6841 cgattgtgga gtcacttcgt gtcctgcttg acgctttctc accacaaaat ctgctgcgcc
     6901 gctttgtgca gtaccgccgc agccatgaac tgcgccagcc gctggatgat gccggagcat
     6961 ggcaaatgta cagccattat tacgaagaac tggcctccga tcgccagcag gggtttgaga
     7021 tgctgtttaa cgaggtctac gcccaggtct atgaccgggt gcttcgtgaa aaacagcggg
     7081 agccggaagc atgaacagga aggcgtttct ggcctgcgtg ctgatgtgcg tcatattaac
     7141 cggctgcgaa acggcgaaaa aaatcagcga ggtgatcaaa aacccggata tccaggtcgg
     7201 gagcctgaag gagcaacctt cggaaataac cgtcaccctg ctgaccgaac cggacaccaa
     7261 taccaatgcg gagggtgaat cagccgccgt ggatgtgcaa ctggtgtacc tgactgacga
     7321 ctcaaaattg ctggcagccg actatgacca gatagccagt acgccgctac cggatgtgtt
     7381 gggtaaaaac tatatcgacc accaggattt caacctgttg ccggacacca tcaaaaccct
     7441 gccgccggta aagctggatg agaaaacgca gtttattggt gtcgtggcct atttctccga
     7501 tgaccaggcc actgaatgga aacaaatcga gacggtggaa ggaaccggcc accattaccg
     7561 cctgctggtg catgtacgtc aaagcagcat cgaaatgaaa aaagaggatg aataattatg
     7621 gcaaccaccc gcaacaaggt gatgtggcag gaagggatgc tgatgcgccc acaccatttc
     7681 cagcaacagc agcgttacaa cgactacctg gataaccagc gtttccgggc catgaatgat
     7741 ttatcctggg gatttaccga actcaccctc aacaatgaac tgctggcgca gggtaagatc
     7801 atgattgaca gcgcgtcagg catactgccc gacggcaccg tcttttctat ccccgaccag
     7861 gacgcactgc ccgatccgct gcacccgcaa aattttccgg acgaaagaag ccgcaatatt
     7921 taccttgctc tgccggtcgc cagtgatgtg agaaatgaaa tcagcgacgg gcggcgaatc
     7981 gggcgttacc ggctgaatta tgccgatgtc cgggatctgc attcagaaga aggtgacgcg
     8041 cgaacactga cgctgggtca actgacgccg cgcattatga gcggtgcaga agatatgagc
     8101 gcctatatta cgctcccgct ttgccgtatc agcgatcgcc atgccgacgg ctccctgacg
     8161 ctggatgacg attttatccc ctcctgccag aatattcagg tcagtaagaa actgcgtgtt
     8221 tatctcaaag aggtacaagg ggccattggc ggacgggcaa gcgatctggc aaaccgcatt
     8281 ggctctccgg cgcagagcgg catcgcggat gtggcggaat ttatgatgtt gcagttactt
     8341 aaccgtaacc agacccggtt tacccatcgc gctcgtcgat cccagctcca cccggaagat
     8401 ttctaccttg atcttgccgg gttgctgggt gaactgatga cctttaccga gccgtcgcgc
     8461 ctgccctgcc cgcttgatgt gtatgatcat catgacctga ccaaaacatt taaaacgctg
     8521 ttaccggaag tcaaacgggc gctgcatacc gtactgtcgc caagagccgt caatctgccg
     8581 ctgcatctgc gtgacggtat ctggcaggcc gatattcatg acacagaact gctgcaatct
     8641 gccacctttg tgctggccgt ggcggcaaac atgccggtcg atcagatcca gcgccagttt
     8701 atccagcagt cgaaaatttc ctcgccggaa aaaatccgca atatggtcag tgtgcagata
     8761 cccggcattc cattgcgtgc cctgatggtg gctccccgcc agcttcctta ccattccggg
     8821 ttcagctatt tcgaactcga caaaagcggc caggcctggg ctgaaatagc tgccgccggg
     8881 gccgttgcac tgcatgtttc cggcagtttc ccggatctga acatgcaact gtgggcgata
     8941 agagggtaag acgatggatg aggggtcact gtctctgcca ccatttaccg gacacgacga
     9001 aaaaagccag cgcgactacc atctcgcgct tcgtggcaac agcctgaatc cgatgatcga
     9061 cgcggccacg ccgctactcg gtatggtaat gcgcctttca accatgaaca gccagacgat
     9121 gccggagcat ctttttgctc aggtggtgac ggatgttcag gccgtggagc aactgcttca
     9181 ggagcagggc tacgaaccgg gcgtgattat ctcgtttcgc tacatcctct gcacgtttat
     9241 tgatgaagcg gcgctcggta acggctggtc gaacaagaac gagtggatca aacagtcatt
     9301 gctggtccat ttccacaacg aagcctgggg tggagagaaa gttttcatcc tgctggagcg
     9361 cctgatccgt gaacccaaac gttaccagga tctgctggaa tttctctggc tctgtttttc
     9421 tctgggattt cgcgggcgct acaaagtggc agcacaggat cagggggaat ttgagcagat
     9481 ctaccgccgc ctgtaccacg tcctgcataa actacgcggc gatgcccctt tcccgctgtt
     9541 acaccaggac aaaaaaaccc agggcgggcg ttatcagctc atcagtcgcc tgaccattaa
     9601 acatattttt tatggtggcg ttgtcgtgct ggcgttgttt tacctgttct atctgttgcg
     9661 gctggacagc cagacgcagg acatcctgca ccagctaaac aaattacttc gataaggacg
     9721 tttatgatcc agattgatct tcccacgctg gtaaaacggc ttaacctgtt ctcccgccag
     9781 gcgctggaga tggccgcctc tgaatgtatg agccagcagg cagcggaaat taccgtcagc
     9841 catgtgctta ttcagatgct cgccatgcca cgcagtgacc tgcgggttat tacccgccag
     9901 ggcgatattg gcatggaaga gttgcgccag gcgctgacgg tggagaacta cacaactgcc
     9961 cgttctgcgg acagctaccc ggcgttttcc ccgatgctgg ttgagtggct gaaagagggc
    10021 tggctgctgg cgtcggctga gatgcagcac agcgaattgc gtggcggcgt gttactgctg
    10081 gccctgctgc attcgccgct gcgttatata ccgcctgctg ccgcccggct gttgaccggc
    10141 attaaccgtg accgtctgca acaggacttt gtacagtgga cacaggagtc ggcggaatca
    10201 gtcgtgccgg atgcagacgg taaaggcgca ggcacactga cggacgctgc tgacaccctg
    10261 cttgcccgct acgccaaaaa catgaccgca gacgcccgta acggcaggct tgacccggta
    10321 ctgtgccgcg accacgaaat caacctgatg atcgacattc tctgccgccg ccgcaagaac
    10381 aacccggtgg tggtgggcga agcaggcgtg ggtaaaagtg ccttaatcga agggctggcg
    10441 ctgcgcatcg tggcaggcca ggtgccggac aaactgaaaa acaccgatat catgaccctt
    10501 gacctgggcg cattgcaggc cggggcgtcg gtgaagggcg aattcgaaaa acgcttcaag
    10561 gggctgatgg cggaggtcat ttcctctccg gtgccggtca ttctgtttat tgacgaagca
    10621 cataccctga ttggcgcggg caaccagcag ggcgggctgg atatctccaa cctgctcaaa
    10681 ccggcgctgg cgcgcggcga actgaaaacc atcgccgcca ccacctggag cgaatacaaa
    10741 aaatacttcg agaaagatgc cgctctgtcg cgccgcttcc agttagtgaa ggtcagcgaa
    10801 cccaacgccg ctgaagccac cattattctg cgtggtctgt cggcggtcta tgaacagtct
    10861 cacggcgtgc tgattgatga tgacgccttg caggccgcag caacgctgag cgaacgttat
    10921 ctctccgggc gtcagttgcc ggacaaagcc attgatgtgc tggataccgc ctgcgcccgc
    10981 gtcgccatta acctttcctc gccgccgaag caaatctcgg cgctgaccac tctgagccac
    11041 cagcaggagg cggaaattcg ccagcttgag cgcgagcttc gcatcgggct gcgtaccgac
    11101 acatcacgga tgaccgaggt gctggtgcag tatgatgaaa cgctgacggc gctggatgaa
    11161 ctggaggccg cctggcatca gcagcaggcg ctggtacagg agattatcgc gctgcgccag
    11221 caattgctgg gcgtggcaga ggacgatgcg gcgccgttgc cggacgcaga taccgtggag
    11281 gatacgccgc cagagtcaga gtcagagtca gagtcagagt cagaacagga taataccggt
    11341 gccgtaccgg ctgatgaggc cgacagagaa cagccggaag agaccgctga aacagtttcc
    11401 ccggtacagc ggctggccca gctcactgcc gaactggacg ccctgcataa cgaccggttg
    11461 ctggtctccc cgcacgtcga taaaaaacag attgcggcgg tgattgccga atggaccggc
    11521 gtgccgctca accgcctgtc gcagaatgaa atgtcggtca tcaccgacct gccgaaatgg
    11581 ctgggcgaca ccatcaaagg ccaggacctg gcgattgcca gcctgcataa gcatctactg
    11641 accgcacgcg cagacctgcg tcgtccggga cgcccgctcg gtgcgttcct gctggctggc
    11701 cccagcggtg tgggtaaaac cgaaaccgtc ctgcaactgg cagagctgct ctacggcggt
    11761 cgccagtacc tgaccaccat caatatgtcc gaattccagg agaaacacac cgtctcgcgg
    11821 ctgattggtt cgcctccggg ctacgttggc tacggtgaag gcggcgtact gaccgaagcg
    11881 attcgccaga aaccctactc ggtagtactg ctcgatgaag tggaaaaagc gcacccggat
    11941 gtgctcaacc tgttctacca gtcgttcgat aagggcgaaa tggcagacgg tgaaggccgc
    12001 ctgattgact gcaaaaatat cgtcttcttc ctgacgtcca acctcggcta ccaggtaata
    12061 gtcgagcacg ccgatgaccc ggaaaccatg caggaagaac tgtatccggt gctggccgac
    12121 ttcttcaaac ctgccctgct ggcgcgtatg gaagtggtgc cgtatctgcc gctgtcgaaa
    12181 gagacgctcg ccaccattat cgccggaaaa ctggcccgtc tggataacgt gctgcgcagt
    12241 cgctttggtg cagaagtggt cattgaaccg gaagtgacgg acgaaatcat gagccgcgtc
    12301 acccgcgcgg aaaacggcgc gaggatgctg gaatcggtca tcgatggcga catgctaccg
    12361 ccgctctcgc tgctgctgtt gcagaaaatg gcggctaaca cggcgattgc ccggattcgg
    12421 ttgtcggcgg tggacgacgc atttacggca gacgtggaag atgctcagaa cgacgagtcc
    12481 gtcacaaagg atgaaacggt tttatgagca ggttgcgcgt tttccggcag acaggatggt
    12541 ttatcgcggg cctgatgacg ggcttacccg ccactgccgc gcccgcagaa acatcgtcaa
    12601 tggccggtgt ggcggtcgcc gtcgccacca cgcccccgcc ggatgcgaca gccacgttac
    12661 aggccatgca gtcctgtcgg cgggagtccg cggcgctgga gcggcttgac tgctatgacc
    12721 gcctgctggc accgctgtcc ccgtcagggt ttgacggggc gctggtcaaa gccggttttg
    12781 tgggcgaggc ctggacccgc gccacagagc aggaaaaacg ccgcgagggc aacaccacag
    12841 aactgctggt gacgcaggtg ccgggggagc gcccgacggt ggtgataacc accccggcta
    12901 tcggccatgt tccgccgcgc ccggtgctga tgttcagttg cgtggacaat atcacccgga
    12961 tgcaggtggc gctgatgcac cctctggatg tccatgatat cgccgtcact ctgaatgccg
    13021 acagccgggc attgcgtagc cactggtttg tgcgcgagaa cggcaccctg ctggagtcca
    13081 gccgggggct gtccgggatt gatgaaatta agcaactgtt cggcgcaaaa acgctgacag
    13141 tggataccgg cgcagataac gccgcaggaa agctgacctt taacattgac gggctggcaa
    13201 gggcgattgc ccccctgcgc gttgcctgcc actgggcggg agaataaaaa tatggcaatg
    13261 aacttacgcg atccgaatgt ctggatatcg cacctgctgg aaaacctgcc ggaagaaaaa
    13321 ctggcatcgg cgctgaaaga tgacaacccg gactgggagt atatcgacgg cgaaatcgtc
    13381 aaactgggat ctctggccca cagtcaactg gatatcccgg aactccagcg ccgggggctg
    13441 gtcattctgg cttcggagag taaggacttt cgtctgctgg cgcatctgct gagaaccctg
    13501 caacacgccg gtgatcctca tctcgcattg cgcctgctgg cgctgtatgt ggagcattac
    13561 tggtcggtgg ctgcgccgca gaatatggca cataaaaaac gctttgccag ccaggtcatt
    13621 aaacgctttg aaacaggtat tgaaggcttt tcacagaacg ccgccacagc gcagcacgac
    13681 gcgctgtcag gggaactggc gaaactggcg cagtgctggc agtcatataa cgccccggaa
    13741 ctggcactgg ctaccgatga tctttttgcc ctgtaccagc gtgcgtttaa tcgtgcggca
    13801 ccggctcctg cccccactcc ggcggcctcc gatagttcac gacagaccac cgccacgtct
    13861 gaaagcggcg tgacgcaacc cagtgctccg gctccccaaa tcgccatcga cagtcacgac
    13921 gacaaagcct ggcgcgacac gctgttaaaa gtggcggcta ttttatgtga acgccagccg
    13981 gactcgccgc agggctatcg cctgcgccgc catgccctgt ggcaatccat caccagtaca
    14041 ccccaggcgg aaagcgatgg acgtacccca ttggctacgg tctctgccga tatggtggcg
    14101 gattaccagt cccggcttgc cagcgcggat atggcgctgt ggcagcaggt tgagaaaagc
    14161 gtattgctgg ctccttactg gctggacggt cactgtcttt ctgcacagac ggcactgcgt
    14221 ctgggttaca aacaggtggc agacaccatc cgcgatgagg tcatccgctt ccttgagcgt
    14281 ctgccccagc ttaccgggct gctgtttaat gaccgcacac cgtttctcag tgagcagacg
    14341 aaacaatggc tggcagcttc gcccgacggc aaagttgcac cggttgcgca aatcggtgag
    14401 gaatcgcagg cagccagagc ctgttttgct gggcagggtc tggaggcggc gctgcgatat
    14461 ctggacatgc tacccgaagg cgatccccgc gatcagtttc accgccagta ccttgccgca
    14521 cagttgacgg aggaggcggg gctgatacag cttgcgcagc aacagtaccg gatgttgttg
    14581 atgataggga gtcagatgat ggtgtctgac tgggagccat cattacttac gcagcttgaa
    14641 caaaaattca cggcagaaca ataaaagaga acaggagttt ttgtgttcag attaccaaca
    14701 ccccgactac tcagcggact caaatcagcc ctacgcccgg cgatgccccg gtttaaagtc
    14761 tccgctttct ggctgctgat actggcgtgg atttttctgc tggtatggat ctggtggaaa
    14821 ggcccgacat ggacgctgta tgaagaacag tggctcaaac cactggcgaa ccgctggctg
    14881 gcaacggcgg catgggggat tatcgcccta gtgtggctta ccgtccgggt gatgaagcgc
    14941 ctgcaacagc ttgaaaaaat gcaaaagcag cagcgcgagg aggccattga tccgctcagt
    15001 gtggagctga acgcccagca gcgttacctt gaccgctggc tgctgcgcct gcaacgccat
    15061 ctcgacaacc gccgtttcct gtggcagttg ccgtggtata tggtcatcgg cccggcgggc
    15121 agtggcaaaa cgacactgct gcgcgagggt tttccgtccg acattattta tgccccggag
    15181 ggcgcacgcg gcgcagaaca acgcctgtac ctcacgcccc atgtcggtaa acaggcggtg
    15241 atctttgata tcgacggcac actctgcgct cccgctgatg cggatatcct gcatcgccgt
    15301 ctgtgggaac atgccctcgg ctggctgaaa gaaaagcgcg cgcgccagcc gctgaacggg
    15361 attattctga cactggattt acccgatctg cttaccgcag acaaacgccg ccgcgagcat
    15421 ctgttacaga cgctgcgcag ccgcttgcag gatatacgcc agcatcttca ctgccagtta
    15481 ccggtttacg tggtactcac ccggcttgat ttattgcagg gttttgccgc cctgttccag
    15541 tcactgaaca gacaggatcg cgatgcaatt ctaggggtca cgttcacccg ccgcgcccat
    15601 gaaaatgatg actggcgaac agagttgaat gctttctggc agacatgggt ggatcgaatg
    15661 aacctagcgt tgccggatct gatggtcgct cagactcaca cccgcgcgtc tttatttagt
    15721 ttttcccgcc agatgcaggg aagccgtgaa ccgctggtgt cactgcttga gggtctgctt
    15781 gacggcgaaa atatgaacgt gatgctgcgt ggtatctatc tcacctcttc gcttcaacgc
    15841 ggacagatgg atgatatttt cacccagtct gccgcccgcc agtaccggct gggcaataac
    15901 ccactggcgt cctggcccct ggtggacacc gcgccttatt tcacccgtgg cctgttcccg
    15961 caggcattac tcgcagagcc taatctggca acagaaagcc gcacctggct gatgcgttcc
    16021 cgtcgccgcc taacggtttt ctctaccaca ggaggcgtgg cggcactgct gctcatcacc
    16081 ggctggcatc actattacaa cggtaactat cagtccggca tcaccgtgct taagcaggca
    16141 aaagccttta tggatgtacc gcctccgctg ggggaagatg attacggcaa cctgcaactg
    16201 ccgctgctga acccggtacg cgatgccacg ctggcctatg gcgactgggg cgaccgcagc
    16261 cgtctggccg atatgggact gtaccaggga cggcgtatcg ggccttatgt ggaacaaacc
    16321 tatctgcaac tgctggagca gcgttacctg ccttcgctgt ttaacgggct ggtcaaagcg
    16381 atgaacgccg cgccgccgga gagtgaggaa aaactcgccg tgctgcgtgt gatgcgaatg
    16441 ctggaggaca aaagcggacg caataatgag gtggtgaagc agtatatggc aaaacgctgg
    16501 agcgaaaaat tccacggcca gcgcgatatc caggcacaac tgatgtccca tcttgactac
    16561 gcgctggctc atactgactg gcatgcagag cgtcaggcgg gcgacggtga cgccatcagc
    16621 cgctggacgc catatgacaa gctcgtggta tcagcacaga aagaactgag caaactgcct
    16681 gtctaccagc gggtttatca gagcctgaaa acgcgggcgc tgggcgttct tcctgctgac
    16741 ctcaatctgc gtgaccaggt agggccaacc tttgaccagg tgtttacatc tgccgatgac
    16801 aacaaactgg ttgttccaca gtttatcacc cgctatggcc tgcaaagcta ttttgtaaaa
    16861 cagcgcgatg aactggttga actgacagcg atggattcct gggtacttaa cctcacccgc
    16921 agtgtgaaat acagtgacgc cgaccgtgcg gaaatccagc accagttgac cgagcagtat
    16981 atcagcgact acaccgccac ctggcgggcc gggatggaca atctgaatat ccgcaatttt
    17041 gagtccatcg gacaactgac cggggcgctg gagcaggtta tcagcggcga ccagcctttg
    17101 cagcgggcgc tgaccgtgct gcgtgacaac acacagccag gcgtcttttc tgaaaaactc
    17161 tctgccaaag aacgggagga agccctggca gagccggatt accagttact cacccgcctc
    17221 gggcatgaat tcgccccgga aaacagtacc ctggcagtac agaaagacaa agaaagcacg
    17281 atgcaggccg tgtatcagca actcaccgag ttgcaccgct acctgctggc aatccagaac
    17341 gcgcctgtac cagggaaatc ggcgctgaaa gccgtgcagt tacggcttga tcagaacagc
    17401 agcgatccga tattcgccac ccgccagatg gcaaaaacgc tgcctgctcc gctcaaccgc
    17461 tgggttggca gactgactga ccaggcctgg catgtggtga tggtggaggc tgttcattat
    17521 atggaagtgg actggcgcga cagcgtggtg aaaccgttta acgagcaact ggcaaataac
    17581 tatccgttta atccgcgttc tgcacaggat gcctcactgg atgccttcga acgcttcttt
    17641 aaaccggatg gcatactgga taccttctac cagcagaacc tgaagctgtt tatcgataat
    17701 gacctgagtc tggaggatgg cgataacaac gtcattattc gcgaagatat tattgcgcaa
    17761 ctggaaactg cgcagaaaat ccgtgacatc ttcttcagca aacagaacgg tctgggaaca
    17821 tcctttgccg tggaaacggt atcgctttcc ggcaataaac gccgcagtgt actgaacctt
    17881 gacggtcagt tagtcgatta cagccagggc cgtaactata ccgcccatct ggtctggcct
    17941 aacaacatgc gcgaaggcaa cgaaagtaag ctgacgctca tcggcaccag cggcaacgcg
    18001 ccgcgcagta tcagcttcag cgggccgtgg gcgcagttcc gcctgttcgg ggccggacaa
    18061 ctgaccggag tacaggatgg caactttacc gtgcgcttta gcgtggacgg tggcgcgatg
    18121 acctaccgtg tgcataccga caccgaggat aacccgttca gcggtgggtt gttcagccag
    18181 tttggtctgt cagacacact gtactgagga ggcagggatg aatagtaacg tactgacaca
    18241 aactatcgtt accggcagtg acccgcgcgg gctgccggaa ttcagcgcca ttcgcgagga
    18301 aataaacaaa gccagccacc cgtcacagcc tgagctgaac tggaaactgg tggagtcgct
    18361 ggcgctggcg atttttaaag ctaacggtgt ggatttacac accgccacct actatacgct
    18421 tgcacggaca cggacacagg gactggcggg cttttgcgaa ggtgcggaac tgctggcggc
    18481 aatggtaagc cacgactggg ataagttctg gccacagggc ggtccggcgc gtactgaaat
    18541 gctggactgg tttaactccc gaaccgacaa tattctgcgt cagcaaatct cctttgcgga
    18601 atccgaccta ccactgatct accgcacaga gcgggcattg cagcttatct gcgacaagct
    18661 ccagcaggta gagctgaagc gcgttccgcg cgtggagaat ctactctatt ttatgcagaa
    18721 cacgcgtaaa cggcttgaac cccagccgaa gagtaacgca gagaacgccg cacagaccac
    18781 ggtcagaacg ctgatttatg ccccggaaac aatggcttcc tccacgccag aagcggtagt
    18841 gcctcccctg cccgacctgc ctgagatgaa agtggaagtg cgcagtctgt cagaaaatcc
    18901 cccacaggcc agtgtgatga agcaaggcag tacggtaaga gggtttatcg cagggatcgt
    18961 ctgttcagtg gctatcgcct cagcattgtg gtggtggcag gtctatccgg cgcagcagca
    19021 actgttacag gttaacgaca ccgcgcaggg cgcagcaacg gtgtggatgg cctcacctga
    19081 acttgaaaac tatgagcgca ggctgcatca gcttcttggc gtctccccgg tacaaccgct
    19141 ggaaaccggg atgcagatga tgcgtgttgc cgacagtcgc tggccggaaa gcctgcaaca
    19201 gcagcaggcc tcgacacaat ggaatgaggc actcaaaacc cgcgcacaga gtagcccaca
    19261 gttgcgcggc tggttgcaga cccgccagga cttacatgct tttgctgatc tcgtgatgca
    19321 gcgcgagaaa gagggattaa ccctttccta tatcaaaaat gtcatctggc aggcggagcg
    19381 gggactgggg caggaaacac ctctggaatc cctgctgacg cagtaccagg atgcccgtgc
    19441 gcagaagcag aatacagatg cgctggaaaa acagattaac gagcggatgg acggcgtgtt
    19501 aagtcgctgg ctgctgctga agaataatac gataccgacg ataaaaaaag acattgaatt
    19561 tcaataacat tcatgaatac aaaggagttt taaatggcga atttaattta tttaacacta
    19621 aatggtgata atcaagggct catttcaagt ggttgttcat cacaaccatc cattggaaat
    19681 aaagctcaaa cagcacacat tgatcaaatt atgatttatg aattcatgca tggattaaat
    19741 cgagatcaaa acgtcaacca tcatcatttg acaataaaaa aa
//
LOCUS       DAZKGF010000091        19754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZKGF010000091 DAZKGF010000000
VERSION     DAZKGF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.091"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            212..754
                     /gene="yfaZ"
                     /locus_tag="ACHOQV_003013"
     CDS             212..754
                     /gene="yfaZ"
                     /locus_tag="ACHOQV_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFP9631353.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            854..2056
                     /gene="yfaY"
                     /locus_tag="ACHOQV_003014"
     CDS             854..2056
                     /gene="yfaY"
                     /locus_tag="ACHOQV_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFP9631354.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLTRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEKQAMEKLWLDVKRVAGQSVIFEGTEGLPSQISRELQTRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2276..3058
                     /gene="yfaX"
                     /locus_tag="ACHOQV_003015"
     CDS             2276..3058
                     /gene="yfaX"
                     /locus_tag="ACHOQV_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP9631355.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     IWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            3073..4278
                     /gene="rhmD"
                     /locus_tag="ACHOQV_003016"
     CDS             3073..4278
                     /gene="rhmD"
                     /locus_tag="ACHOQV_003016"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFP9631356.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VISFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYNH"
     gene            4335..5624
                     /gene="yfaV"
                     /locus_tag="ACHOQV_003017"
     CDS             4335..5624
                     /gene="yfaV"
                     /locus_tag="ACHOQV_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9631357.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKEEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5641..6444
                     /gene="yfaU"
                     /locus_tag="ACHOQV_003018"
     CDS             5641..6444
                     /gene="yfaU"
                     /locus_tag="ACHOQV_003018"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFP9631358.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAHVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAADKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGNY"
     gene            complement(6485..6718)
                     /locus_tag="ACHOQV_003019"
                     /pseudo
     CDS             complement(6485..6718)
                     /locus_tag="ACHOQV_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6719..7714)
                     /gene="rpnE"
                     /locus_tag="ACHOQV_003020"
     CDS             complement(6719..7714)
                     /gene="rpnE"
                     /locus_tag="ACHOQV_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFP9631359.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDSEGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKIEGWQ
                     EGKLEGLQEGKLDGLQEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCH"
     gene            complement(7907..9097)
                     /gene="glpC"
                     /locus_tag="ACHOQV_003021"
     CDS             complement(7907..9097)
                     /gene="glpC"
                     /locus_tag="ACHOQV_003021"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFP9631360.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9094..10353)
                     /gene="glpB"
                     /locus_tag="ACHOQV_003022"
     CDS             complement(9094..10353)
                     /gene="glpB"
                     /locus_tag="ACHOQV_003022"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFP9631361.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLEALRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWSLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQLFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQL"
     gene            complement(10343..11971)
                     /gene="glpA"
                     /locus_tag="ACHOQV_003023"
     CDS             complement(10343..11971)
                     /gene="glpA"
                     /locus_tag="ACHOQV_003023"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFP9631362.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQRIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTMSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12244..13602
                     /gene="glpT"
                     /locus_tag="ACHOQV_003024"
     CDS             12244..13602
                     /gene="glpT"
                     /locus_tag="ACHOQV_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFP9631363.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            13607..14683
                     /gene="glpQ"
                     /locus_tag="ACHOQV_003025"
     CDS             13607..14683
                     /gene="glpQ"
                     /locus_tag="ACHOQV_003025"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9631364.1"
                     /translation="MKLTLKNLSMAIMMSGMIMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYSGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            14886..15536
                     /gene="inaA"
                     /locus_tag="ACHOQV_003026"
     CDS             14886..15536
                     /gene="inaA"
                     /locus_tag="ACHOQV_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFP9631365.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKSEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(15590..15844)
                     /gene="yfaE"
                     /locus_tag="ACHOQV_003027"
     CDS             complement(15590..15844)
                     /gene="yfaE"
                     /locus_tag="ACHOQV_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFP9631366.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(15844..16974)
                     /gene="nrdB"
                     /locus_tag="ACHOQV_003028"
     CDS             complement(15844..16974)
                     /gene="nrdB"
                     /locus_tag="ACHOQV_003028"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9631367.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(17040..19325)
                     /gene="nrdA"
                     /locus_tag="ACHOQV_003029"
     CDS             complement(17040..19325)
                     /gene="nrdA"
                     /locus_tag="ACHOQV_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9631368.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            19453..19596
                     /gene="ypaB"
                     /locus_tag="ACHOQV_003030"
     CDS             19453..19596
                     /gene="ypaB"
                     /locus_tag="ACHOQV_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFP9631369.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     VIS"
BASE COUNT         4544 a         4885 c         5380 g         4945 t
ORIGIN      
        1 agtaatctct tttgcgatga taactgcact tattattatt tttgtgtgta tcggtaaact
       61 gtgtttattt tttcggcgtt taattattta agaagtattt agttacaata agtgactaga
      121 tccctttttt cgctcattga atcctctgcg ccaggcagta caatcccgca gctttgggaa
      181 ttttgattaa ttgcttgtgg gaaaggggat aatgaaaaaa attgcgctag caggtctggc
      241 cggaatgctg ttggtttctg catcggtcaa tgcaatgagc atcagcggcc aggcgggtaa
      301 agaatacacc aatattggtg tcggttttgg gactgaatcg acgggcctgg cgttaagcgg
      361 taactggaca cataacgacg acgacggtga cgtcgcgggc gtggggctgg ggttgaatct
      421 gcctctcggg ccgttaatgg cgaccgttgg cggaaaaggc gtttacacca acccgaatta
      481 cggcgatgaa ggttatgccg cagcggtagg aggtggtttg cagtggaaaa ttggcaacag
      541 cttccgcttg tttggcgagt attactactc tccggattcg ctctccagcg gtattcaaag
      601 ttatgaggaa gcgaatgctg gcgcgcgtta caccattatg cgtccagtca gtattgaggc
      661 gggttatcgc tacctgaacc tttctggtaa agacggtaac cgcgacaacg cagtggctga
      721 cggcccgtat gtgggggtta acgccagttt ttgatccctt ccggcgcggc attctgccgc
      781 gccattcagt ttcttactac gccttatctc cctttgcgtt atagtgattc gataacaaaa
      841 gcaggagata accatgttaa aagtggaaat gttatccacc ggggatgaag tgttacacgg
      901 gcaaatcgtt gacactaacg ctgcctggct ggccgatttt ttctttcatc aggggttgcc
      961 tttaactcgc cgcaatacgg tgggggataa ccttgatgac ttagtgacca ttcttcgcga
     1021 acggagtcag cacgccgatg tgctgatcgt taacggcggg ctggggccga ccagtgatga
     1081 tttaagcgca ctcgccgctg cgacagcaaa aggtgaaggc ctggtgctgc atgaggcctg
     1141 gctcaaagag atggaacgct atttccacga acgtggacga gtaatggcac cgagcaaccg
     1201 taaacaagcg gagctgcctg ccagtgctga atttatcaat aacccggtag gcaccgcctg
     1261 tggttttgcc gtgcagctta atcgttgcct gatgttcttt acccccggcg taccgtcaga
     1321 atttaaagtg atggtcgagc acgaaatcct gccgcgcctg cgcgagcgtt tttctttacc
     1381 gcagccgccg gtttgtctgc gtttgactac atttggtcgt tcggaaagcg atctggcaca
     1441 aagcctggac accctgcaac tgccgccggg cgtaacaatg ggctatcgct cttctatgcc
     1501 gattatcgaa ctgaaactca ccggaccggc aagcgagaaa caggcgatgg aaaaactgtg
     1561 gctggacgtt aaacgtgttg ccggacagag cgtgattttc gaaggtaccg aaggactgcc
     1621 ctcgcagatc agccgcgaat tgcaaacccg ccagttcagc ctgacgttga gcgagcaatt
     1681 cacaggtggt ttattggctt tgcaactttc tcgcgcaggt gctccattgc tggcgtgtga
     1741 agtggttcct tcacaggagg aaaccctggc gcaaactgcg cactggatta cagaacggcg
     1801 ggccaaccat tttgccgggc tggcactggc tgtttcgggt ttcgagaacg agcatctcaa
     1861 ctttgcgcta gccacgccag atggcacttt cgctctgcgt gtgcgtttca gcactacacg
     1921 ctacagcctg gctatccgtc aggaagtgtg cgcaatgatg gcactgaata tgctgcgccg
     1981 ttggttaaac ggccaggaca tcgccagtga gcatggctgg attgaggttg ttgagtccat
     2041 gaccttatct gtctgattcc gccactctgt gccggtttac tgcaccggca cattccccct
     2101 ccggcaaaat ttgtccaacc tgttgtactc aataagaaaa aatccgtgcg ctaattcaca
     2161 aatttgtgcg taatttctcc agtccgctgg acgaggagcg ggatttgatg agcttcacaa
     2221 ttttactcca cctgagcaac gagactgatt ccagtatcta agactggagt ccagtatgtt
     2281 ggaatcaagc aaagttcccg ccctgacacg ggctattgat attctcaatt tgattgcccg
     2341 cattggcccc tgtagtgctg cgacgatcat tgacacattg gggatcccta aaagcacggc
     2401 ctatttgctg cttaatgaac tcaggcgtca gcgttttctt agtcttgatc accaggaaaa
     2461 tttttgtttg tggaccaggc tggtggagct ttccggtcat gcgttaagca agatggatct
     2521 ccgggaactg gctcgaccgc gtttgacgca actgatggat accaccgggc tgttatgtca
     2581 cctggggatt attgataacg gcagcgctta ttacattttg aaagtggagt catcagccac
     2641 tatcagcgtg cgttcccatg aaggaaaaag cctttcgctt tatcgctccg gaataggtaa
     2701 atgcctgctt gcctggcaac ctgcagctgt acagcaaagt attatcgaag ggctgatctg
     2761 ggagcaggcg acacccacaa cgattactca tccacagcaa cttcacgaag aactggcgcg
     2821 tattcgccgt cagggctgga gctatgacaa cggcgaagat tacgccgatg tccgttgtgt
     2881 cgctgcgccg gtgtttaacg ccaataacga gcttaccgct gcgatctcgg tggtcggtac
     2941 ccgcttacaa atcaatgaag aataccgtga ttatctggcg ggcaaggcca ttgcctgcgc
     3001 cagggatatt tcacgtctgt tgggatggaa aagtcccttt gacttacagg cctcataaca
     3061 atggagaaca gcatgaccct accaaaaatc aaacaggttc gcgcctggtt tactggcggt
     3121 gcgacagcag aaaaaggcgc tggcggcggt gattatcacg accagggggc aaatcactgg
     3181 attgatgatc atattgccac cccgatgagt aaataccgcg attacgagca gtcacgccag
     3241 tcatttggca ttaacgttct tggcacgttg attgttgaag tcgaagcaga aaacggccag
     3301 accggattcg ccgtttcgac agccggtgaa atgggctgtt ttattgtcga aaaacacctt
     3361 aaccgtttca ttgagggtaa atgtgtcagt gatatcaaac tgatccacga tcaaatgctc
     3421 aatgccaccc tgtattactc cggctctggt ggcctggtga tgaacacgat ttcctgtgtc
     3481 gatctggctc tgtgggatct gttcggcaaa gtggtcgggc ttccggttta taaactttta
     3541 ggcggcgcag ttcgtgatga gattcagttc tacgccacag gtgcgcgtcc ggatctggca
     3601 aaagagatgg gctttatcgg tggcaaaatg ccgacgcact gggggccaca tgatggcgat
     3661 gcggggatcc gcaaagatgc cgctatggtc gcggatatgc gtgaaaaatg cggtgaggat
     3721 ttctggttaa tgctcgactg ctggatgagt caggacgtga actatgccac caaactggcc
     3781 cacgcttgcg cgccctataa cctgaaatgg atcgaagagt gcctgccgcc acagcagtat
     3841 gaaggttatc gcgaactgaa acgcaacgcg ccagtcggaa tgatggtcac cagcggtgag
     3901 catcacggca cactgcaatc tttccgtacg ctttcagaaa ccggtatcga cattatgcag
     3961 ccggatgttg gctggtgcgg tggcttaacc acgctggtgg aaattgccgc aatcgccaaa
     4021 tccagagggc aactggtggt gccgcacggt tcgtctgttt actctcacca tgcggtgatc
     4081 agcttcacca atacgccatt cagcgaattc ctgatgacca gcccggattg ttcaacgatg
     4141 cgtccgcaat ttgatccgat tctgcttaat gagccagtgc cggtgaatgg tcgtattcat
     4201 aaatcagtgc ttgataaacc cggtttcggc gtcgaactca atcgtgactg caatttgaaa
     4261 cgcccctaca accactaatc actccgggcg ttgctgtgac aacggtaacg cccacatatt
     4321 ctaaggagct ttttatgagc accgctttgc ttgacgccgt ggtgaagaaa aaccgtgcgc
     4381 gtttaattcc gtttatgttg gcgctgtatg tgctggcgtt tctcgaccgt tcgaatatcg
     4441 gttttgccaa acagacctac cagattgata ccgggctgag taatgaagct tatgcgctgg
     4501 gagcaggcat tttctttgtg gtatatgcgt ttctaggtgt tccggcgaat cttttgatgc
     4561 gcaaactggg ggccagaacc tggattggta cgacaacact gctgtgggga tttctttcgg
     4621 cagccatggc atgggccgat actgaagcga aatttctgat cgttcgcact ctgcttggtg
     4681 ctgcggaggc cgggttcttc cctggtatga tttatctcac ctcgcaatgg tttccgcagc
     4741 gtaatcgcgc cagcattatg gggctgttct atatgggcgc accgttggcg ttaacactgg
     4801 gatcaccgct ttccggcgcg ctgctggaga tgcatggatt tatgggacat cccggctggt
     4861 tctggatgtt tgtgattgaa ggattgctgg cagtcggcgc tggggtattc acattctttt
     4921 ggcttgatga cacaccggag caggcacgtt ttctgagtaa agaagaaaaa acattgctta
     4981 tcaatcaact ggcaagtgaa gaacaacaga aagtgacttc tcgactgagc gatgcgctgc
     5041 gtaatgggcg agtctggcaa ctggcgatta tctacctgac cattcaggtg gcggtttacg
     5101 gattaatttt cttcctgccg acccaggttg ctgcattgct ggggacaaaa gtgggcttta
     5161 cggcgtcggt ggtcaccgcc attccgtggg ttgcggcctt gtttgggacc tggcttattc
     5221 cgcgctattc cgataaaacc ggcgaacggc gtaatgtcgc agcgctgaca ttactggcgg
     5281 caggcattgg tattggtctc tccgggctgc tttctccagt actggcgatc gtagcgctgt
     5341 gtgttgcagc catcgggttt attgccgtgc agccggtgtt ctggacgatg ccgacacaac
     5401 tgctttccgg tacggcgctg gcggcgggga ttggttttgt aaacctgttt ggtgcagtgg
     5461 gtgggtttat tgccccgatc ctgcgcgtga aagcagaaac gttgtttgcc agcgatgcgg
     5521 cgggattact gacgctggca gcggtggcgg tcatcggttc gctgattatt ttcactctgc
     5581 gtgtaaatcg cactgttgcg cagaccgacg tggcacatca ttaataggtt aaggaacacg
     5641 atgaacgcat tattaaccaa tccctttaaa gaacgtttac gcaagggcga agtgcaaatt
     5701 gggctgtggt taagctcaac gactgcctat atggcagaaa ttgccgctac ttccggttat
     5761 gactggttgc tgattgacgg ggagcacgcg ccaaacacca ttcaggatct ttatcaccag
     5821 ctacaggcgg tagcgcccta tgccagccaa cccgtgatcc gtccggtgga aggcagtaag
     5881 tcgctgatta aacaagtcct ggatattggc gcgcaaactc tactgatccc gatggtcgat
     5941 actgccgatc aggcacgtca ggtggtgtct gccacgcgct atcctcccta cggtgagcgt
     6001 ggtgtcgggg ccagtgtggc acgggctgct cgctggggac gcattgagaa ttacatggcg
     6061 cacgtcaacg attcgctttg tctgttggtg caggtggaaa gtaaaacggc actggataac
     6121 ctggacgaaa tcctcgacgt cgaagggatt gatggcgtgt ttattggacc tgcggatctt
     6181 tctgcgtcgt tgggctaccc ggataacgcc gggcacccgg aagtgcagcg aattattgaa
     6241 accagcattc ggcggatccg tgctgcggat aaagcggctg gttttctggc tgtggctcct
     6301 gatatggcgc agcaatgcct ggcgtgggga gcgaactttg tcgctgttgg cgttgacacg
     6361 atgctctaca gcgatgccct ggatcaacga ctggcgatgt ttaaatcagg caaaaatggg
     6421 ccacgcataa aaggtaatta ttgatatcaa aggcccatgg gggtcggctg tgggcctgtg
     6481 ttaattagtg gttattcgct gccagatcgg cttcgcttag ctgggtggct gcgagcacca
     6541 ggtcacgatc aatgccctgt accagcatgc gcaaagcaat tttaatggct tgtttacgca
     6601 taccttcctg caaaccttct aatttaccct cctgccagcc ttcaagctta ccttcctgcc
     6661 agccaatctg atgcccttcc tgcctcagcc gttcagcaat agtcatcaat ctctcctttt
     6721 aatgacagtt cggaatatca gcatcagtga gttgggttgt cgccagcaca atctcacgtt
     6781 caaagccctg ctccagcatg cgcaaggcaa ttttaatggc ttgttcatgc atgccttcct
     6841 gcaaaccatc taatttacct tcttgcaaac cttctaattt accttcctgc caaccttcaa
     6901 ttttaccttc ctgccagcct tcaatcttac cttcctgcca gccaatttga tgcccttcct
     6961 gccgcagccg ttcagcaata gtcattaatc tctccttttg tagtggtgaa cgttcggcaa
     7021 tctcctcaat aaaacgggtg aaacgggagg tatcgccgca ttgcagcaga taattaaaca
     7081 gcgtttgtag ctggctgtca ttagtgaagc ctctaaccaa aagcgtggtg atcctgtcga
     7141 ccataccgat taaatcgcgg tcgcgaatat gcttttgaat cagttcaagc agagctatac
     7201 gccgatgttg catgatctca tcgtcaggta cgatggtgat atccaccaac ggaaaagctt
     7261 cggtgtacaa ctgccgggca agttgcggat cgtcaaactc atccagccag ttgagtgagt
     7321 acgggtaggg cgaggtttcg ccatgataaa acagcaatgg caccaccagc ggaactctgt
     7381 catagccttt atccaggtga cgctgcatgg cggcagtggc atagcgcatt agccgaaaag
     7441 ccatattctt ttctgcagag ctttgatgtt caatcacgca gtagatatag ccttcactgt
     7501 cgctggtttc cacagaccac aaaacatccg agtagtaagc gcgtaaactt ttttcaataa
     7561 agctggtggg ttccaggcgt aaagtttgca ggttgcaaag cttgcgcagc ggctctggta
     7621 gatgtatttc cagaaaatcc cgagcggttt cgggtgtgaa cataaaggtt ttaaacaccg
     7681 catcatgcgg ggaggaggtt gttgattcgg tcatggttgt ccgtcacctg atatttatga
     7741 tgacgcgaca ttacggttat cccaggcctt tgacatctgc cagtttcagc ctttgcgaga
     7801 cgttttcaga ggaattttca ctgcgttgca acgcatacat tgggcacgga atcgtgtacg
     7861 caaaaatgcc cggtaacaac atgcttaccg ggcatcagaa aggagtttaa gccagcgcct
     7921 gggccagcag cgtaatcgga tgttcgcagc gaagacttgt ggacatctca atctgccatt
     7981 tgcaggtttc gcagtcggta accaccagat ctgcgccgct ttcttctatc tgacggaaca
     8041 gtggtgcgcc gatggcttgt gaggtggggt agttctcttt tttgaaaccg taagtacctg
     8101 caataccgca gcactgggaa tccagcaccg ttaactcaag ccccgggatt ttacgcaaca
     8161 gctccagggt gtagagcgtc cagcccattt tttccatatg gcacggagtg tgataaacca
     8221 ctttcagcgg cagcggtttc agcggcaagg ttttgccttc gtccagcttg cgccacagcc
     8281 agcgggtcgc cagttcgatg tgatcgcgca agcctttgtt gtcgacattc agcacttccg
     8341 gatattcgtc gcgcagggca aatgtacagg ttgaggaggt ggcaatcacc ggaatgcctt
     8401 ttactcccac tgcttcgcgg atcgactcta catttgtgat tgcctgttta cgtgctttat
     8461 cggtaaagcc gttggcgatc agcggtacgc cgcagcattt ttctttgctg agcagttgta
     8521 caccggtacc cattgcgttg agcactttaa ttaaatcttt gcctaactgc ggatggttgt
     8581 agttaacgaa gcagccgtga aagaaagcca cctggtcttt atattgtgcc tgctgggccg
     8641 ccacgctgcg ataccagcga cggaacgtac cgaaggagta tttcggtagc gtgcggcgat
     8701 gatcgatttt taacgccgca tcaagcagct gccgcactgg tttcagcgag gtagcggtat
     8761 tgacgattgg tgcgaatggc gtggaaacgc tacccatcag gtcggtatga ctcaacacaa
     8821 aattgcgcag cgacgggcgt gtggtgtcat atttcgcccg cgcgcgctgg atgatatcgc
     8881 caatcttcac atcggacggg caggcgactt cgcagcgttt gcagttgatg caatatttca
     8941 gcgcttcgtc atacagtgcg ccatctttca gacgcagacg ctcgccatcc ggcccggctt
     9001 gttttggccc tggatagccg ggattcaccc ggctcaccgg gcaggcggtg gtgcagacgg
     9061 tgcacttaat gcagttttcg aagctggtgt cgttcatagt tggcctcctg cgcgttgggc
     9121 aatctgttga gcggcatgta aagcactgac ggcacaaaca ccgccgccgc atccctgggc
     9181 gatgggatca aatccgccca gcaccgaacc gatggcaaac aggttctcaa tggtttgccc
     9241 tgcctgcgat gggcgtagtg cctcatcagt ggttacaccg aacagctgcc acggttgcgg
     9301 cgcaaaaaaa tcgcccttat accattcacc ccgcgtggcg gtttgtagca catcaaggcc
     9361 gagaatcggc tcgcgaatgc cgtcacgttc tgccaccagt ccgccgctaa agaaactgcc
     9421 gctcgcaagc accgcaaatc gtgggcgcag cgggatatcg gcgtgattgc gcgtccagat
     9481 ttcattcact acgccatttt tacaggtcac ttttttcact tcatcgcctg gcatccacac
     9541 gccaccctgg cgcacaaact ggcgctgtaa ctggttttgc agacgaatgc ccagcacgga
     9601 aggcggcagc gttggcaaaa gcatcagtga acaaggtagt ttttcattca accaacgcca
     9661 cagtttgtca tcggccaggc cgaagcaggc gggcatcagg atcatttcgc aggtattggc
     9721 gacaggaata agcgcatcaa gtaacagcga ccagttctct tcgttatcaa ggaaacgggc
     9781 gatattcacc gcgcgaaact cggtggcgtt gttgcgcagc acatccagtt ccggcagctc
     9841 tatttctgcg gtttcaacgg caaggtcgag ttcgcgcaac gaagccgccg caagatgcgc
     9901 ctgaaaatcc atcaggccgc ttatcccgac cacacatatt ttcttcgcgg gcaacggcca
     9961 gacaggaact tctggcgaac ttagccaggt tgagcgcaga gtgccgagcg gggtaactcg
    10021 ctggtgggcc agttctacgc tgccctgtaa ttgcgcaccg ctttcagcga ttaatgcctg
    10081 cgcctggcaa gcgagatcga gtacgcgttg tggcccgaga agagtgtaag gatgggcagg
    10141 tgcctgctga cgcaaagctt ccagtccact gtgaatgtct gtcaccggtt gaccatccgg
    10201 cagatggctc agcaaatcca gcgatccaga tgagaaatgg agcgcgcttt gaccacgcgt
    10261 gacaatggca cagcgcaggc cgtgtttttg cagttgcagg ccacagagta atccggcgag
    10321 gccgccgccc ataatgacag tatcaaagcg catctttctg ctccttctcc agaccacaca
    10381 gtccctgata aacccagcgg gtaaattcgc tttcgcgcag tgcatctccc caggcgatgg
    10441 gttgcacgcc tttccagcgt tcgttaagga aggtggaaag ttgctcgata gattgcgcgg
    10501 acatcgtgac gttaaaacgt tgcagcagtc cggcagcgcg gcaggcgcag agttcgccct
    10561 ggcaggtacc catccccaca cgggtacgac ggcgtaaatc cagcaggcta ttaacgttta
    10621 aattttctac cgcgtactga acttcacccg cagttaccgc ttcgcactca cataccaggc
    10681 tacggtgcag acggccttcg ctcagccagg cgggtgtgcg atcgccatga cgataaaccg
    10741 cagaaccgcg cagcggggca ggcagggaga tgactttacg caaggtaact tcagcgggct
    10801 cctgtgaacc aggaagtgcc agatcggcgg ttgtacaggg gcgcgtgttg cccagcttgc
    10861 gacataccgc gtcggtagcc cattccgcca tcagccgata ggtcatcagt ttgccgccgg
    10921 tgatggtgat aaatccgtcc agaccatcgc gttcagcatg gtcgagcagc acgatgccac
    10981 gactgacgtt acggccgctc gggtcgtcat cgctggcaac cagcggacgc acgccagagt
    11041 aagcccgcaa aatacgcgtt ttcgccatca cgggggccag tttttcccct tcacgcagca
    11101 gaatatcaac ttcttctgcc gtcactcgat tatcgtcaat ctcattgtaa tcaatacgta
    11161 aagaggtggt gccaattagc gaaatggtat cgccaggcac cagaatatcg gcgtcggaag
    11221 gtttgcggca gcggttgatc acatgctgat taatgcggtg atccatgatc agcagcgatc
    11281 ctttcgccgg gaacatgcga atgcgcagat cggcatattc ggcaatgcgt tgcccccaga
    11341 tcccagcggc attaaccacg acaggcgcat gaagggcctg agtttcgccg gtgagatggt
    11401 tacgtacacg aacgccgcac accgtcgcac cttcacgaat cagccccgtg acttcatgag
    11461 cggtaaggat aacggcaccg tgttctttgg catccagcat gtttgctgcg gtcagacgaa
    11521 atggatcaac ggtgccatcc ggaactttca ccgcgccaat cagtgccggg ttaacggcag
    11581 gttcgataat gcgcgcttgc tgcgggtcta tagcttctgc gctgatccct gcttcttcgc
    11641 aggcgcggat aaaagtggcc tggaaggaga ggtcatcttc cggcagggtg ataaacaggc
    11701 cgttggtcgg ttcaacgcag tgacgtgcaa tgcgtttcag gatctggttt tcactaatac
    11761 attcgcgggc cgattccgca tcggttaccg catagcgcgc accgctgtgc agcaggccgt
    11821 ggttacgccc ggtggcaccg gttgcgatgt cgtggcgctc aaccaaaatc acgcgcagtc
    11881 cgcgcagggc acagtcgcgg gcaatcccgg ctcccgttgc gccgccgcca atgataatca
    11941 cgtcacttga ttgcgagtcg cgagttttca ttgttatccc tctgaagttc gttttttacc
    12001 atttagccat agtaaaaaca tgaattgttt gatttcgcgc atattcgctc ataattcgaa
    12061 agtgaaacgt gatttcatgc gtcattatga acatttaacc catcgaattt aataatgtgt
    12121 gcggtaattc acatttaatt tatgaatgtt ttcttaacat cgcggcaact caagaaacgg
    12181 caggttctcc cactgaatca ggctgttaat cataaataag accacgggcc acggaggcta
    12241 tcaatgttga gtatttttaa accagcgcca cacaaagcgc gcttacctgc cgcggagatc
    12301 gatccgactt atcgtcgatt gcgctggcaa attttcctgg ggatattctt tggttatgct
    12361 gcttactatt tggttcgtaa gaactttgcc ctcgctatgc cttatctggt cgagcaggga
    12421 ttctcacgcg gtgatttagg ttttgccctt tcggggatct cgattgctta tggattttcg
    12481 aaattcatca tgggctcggt gtcggatcgc tcgaatccgc gcgttttcct gcccgcaggt
    12541 ttgattctgg cggcggcggt gatgcttttt atgggctttg tgccgtgggc gacgtcgagc
    12601 attgcggtga tgtttgtgct gctgttcctc tgcggttggt tccaggggat gggatggccg
    12661 ccgtgcggtc gtactatggt gcactggtgg tcgcagaaag aacgtggcgg cattgtgtca
    12721 gtgtggaact gtgcgcataa cgtcggtggt ggtattccgc cgctgctgtt cctgctgggg
    12781 atggcctggt tcaatgactg gcacgcggcg ctctatatgc ccgctttctg cgccattctg
    12841 gtggcactgt tcgcctttgc gatgatgcgc gataccccgc aatcctgtgg cctgccgccg
    12901 atcgaagagt acaaaaatga ttatccggac gactataacg aaaaagcgga acaggagctg
    12961 acggcgaagc aaatcttcat gcagtacgtg ctgccgaaca aactgctgtg gtacatcgct
    13021 atcgccaacg tgttcgttta tctgctgcgt tacggcatcc tcgactggtc accgacttat
    13081 ctgaaagagg tgaaacactt cgcgctggat aaatcctcct gggcctactt cttctatgag
    13141 tatgcgggca ttccgggcac cctgctgtgc ggctggatgt cggataaagt cttccgtggc
    13201 aaccgtgggg ccaccggcgt cttctttatg acactggtga ccatcgcgac tatcgtttac
    13261 tggatgaacc cggcaggtaa cccaaccgta gatatgattt gtatgattgt tatcggcttc
    13321 ctgatctacg gccctgtgat gctgatcggt ctgcatgcgc tggaactggc accgaaaaaa
    13381 gcggcaggta cggcagcggg ctttaccggg ctgtttggtt acctgggcgg ttcggtggcg
    13441 gcgagcgcga ttgttggcta cacagtggac ttcttcggct gggatggcgg ctttatggta
    13501 atgattggcg gcagcattct ggcggttatc ttgttgattg ttgtgatgat tggcgaaaaa
    13561 cgtcgccatc aggaattact gcaaaaacgc aacggaggct aatggcatga aattgacgct
    13621 gaaaaacctt agcatggcga tcatgatgag tggaatgatc atgggaagca gtgcaatggc
    13681 ggcggacagc aacgaaaaaa tagtcatcgc ccatcgcggt gccagtggat atttgccgga
    13741 gcatacgctg ccagcaaaag cgatggcgta tgcgcaggga gcggattatc tggaacaaga
    13801 tttggtgatg accaaagacg accatctggt tgtcctgcat gaccattatc tcgatcgtgt
    13861 tactgatgtt gccgatcgtt tcccggatcg ggcgcgcaaa gacggtcgtt actacgcgat
    13921 agatttcacg ctcgatgaaa ttaagtcact gaaatttacc gaaggtttcg atattgaaaa
    13981 tggtaagaaa gtacagactt atccggggcg tttcccgatg ggtaaatctg acttccgggt
    14041 acacactttc gaggaagaga ttgaatttgt tcaggggtta aatcactcta ccgggaaaaa
    14101 tatcggtatc tatccagaaa ttaaagcgcc gtggttccat catcaggaag ggaaggatat
    14161 tgcggcaaaa acgctggaag tgctgaagaa atatggttac agcggtaagg acgacaaagt
    14221 ttatttgcaa tgttttgatg ccgatgaact gaagcgtatt aagaatgagc tggaacctaa
    14281 aatgggcatg gacctcaatc tggtacagct gattgcctat accgactgga acgaaactca
    14341 gcagaaacag ccggacggaa gctgggttaa ttacaactac gactggatgt ttaagccggg
    14401 ggccatgaaa caggtggcgg aatatgctga tggtattggt ccggattatc atatgttgat
    14461 tgaggagaca tcgcagccag gtaatatcaa actcactggc atggtgcaag atgctcagca
    14521 gaacaagctg gtagtgcatc cttataccgt gcggtcagat aaactgcctg aatacactac
    14581 tgatgtgaat cagttatatg atgctctgta taacaaagcg ggtgtaaatg ggctatttac
    14641 tgatttccct gataaggcag taaaattcct taataaagag taaattaact accggatggc
    14701 acttatgtgc tatccgatac cactcttcat taaagtctgt gggttatcct taattgcaat
    14761 tattttgagc gagatgactt gttcccggca taagatactc gtcaatacaa cacgtattat
    14821 taagattttc ctcagctaaa catctatagt cattgtcgtt aataaaaaac gacaggcgat
    14881 agggtatggc agtttcagca aagcacgacg aatttaatca ctggtgggca acagagggcg
    14941 actgggttga agagcctaac tatcgccgta atggtatgag cggggtgcaa tgcgtcgagc
    15001 gcaacggcaa aaagctatat gtaaagcgca tgacgcatca tctgtttcat tccgtacgtt
    15061 atccgttcgg ccgaccaacg attgtccgtg aggttgcggt aattaaagaa ctggaacggg
    15121 ccggtgtcat cgtgccgaag atcgtttttg gtgaagcggt gaaaattgag ggggaatggc
    15181 gcgcgttgct ggtgactgaa gatatggcgg ggttcatcag cattgctgac tggtatgccc
    15241 agcatgcagt aacgccctat tctgacgaag tacggcaagc catgttgaaa gcagtggcgc
    15301 tggcgtttaa gaaaatgcat agcgttaatc gtcagcatgg ctgttgctat gttcgccata
    15361 tctatgtgaa aacagaaggt aagtcagaag ctggttttct tgatctggag aaaagtcgtc
    15421 gccgcttgcg tcgcgataaa gcgattaatc atgacttccg ccagctggaa aaatatctgg
    15481 agccgatccc taaagcggac tgggagcagg tgaaagcgta ttactacgcc atgtaatcgt
    15541 tgtaggccag ataatacgcg taagcgtcgt atctggcgct agtacccgat cacaactcga
    15601 tttcaatatc gccttttgcc cggcaacaac agggcaaaat ttcccccggc tgaataaagg
    15661 ctaacggttc ggcaatccag tccacctggc ctgcaacaag gcgcgtgcga caggaaccgc
    15721 agtaaccttc gcggcactgg tactcaaccg ccacattgtg ggattccagc gccgccagaa
    15781 gggaagggtg ttcatcctgg cacagcagtt gtgtgccagt gatgcgcagg gtaacgcgag
    15841 ccatcagagc tggaagttac tcaaatcgtc ggtgtccact tccgagtcaa tctgcccgac
    15901 cagataagaa ctgacttcca cttcctgcgg agcaacctgc acgttatcag acaccagcca
    15961 ggtattgatc cacgggatcg ggttggagcg tgtctggaac ggcagatcca aaccgactgc
    16021 ctgcatgcgg atattggtga tgtattcaac gtactggcag agaatgtctt tattcagacc
    16081 aatcatcgaa ccgtcacgga acagataatc cgcccagtct ttctcttgct gtgccgcctg
    16141 gacaaacagg tcatagcact cctgcttaca ttcttcggca atttccgcca tctccggatc
    16201 atccgcgccg ctgcgcagca gattcagcat atgctgagta ccggtcaggt gcagggcttc
    16261 atcgcgagca atcaggcgaa taattttggc gttaccttcc atcagttcgc gttctgcaaa
    16321 ggcaaaggaa caagcaaagc tgacgtagaa gcggatcgct tccagcgcgt taacgctcat
    16381 caggcagaga tacagttttt tcttcagctc gcgcaggcta acggttacgg ttttaccgtt
    16441 aacggtgtgg gtaccttcgc ccagcagatg ccagtagctg gtcatttcga tcagctcatc
    16501 gtaatagctg gagatccctt ccgcacgttt ctggatctgc tcgttggtga cgatatcgtc
    16561 aaacacaaca gacggatcgt taacgatatt acgaatgata tgggtataag aacgagagtg
    16621 aatggtttct gagaacgccc aggtttcgac ccaggtttcc agctcaggga tcgagatcag
    16681 cggcagcagt gccacgttcg ggctgcgacc ctgaatggag tccagcagcg tctgatattt
    16741 caggttgctg ataaagatgt gtttttcgtg ttcaggtagt gcctggtaat cgatacggtc
    16801 acgagaaacg tcaacttctt ccggacgcca gaagaaagag agctgctttt cgatcagctt
    16861 ttcgaagatg tcatattttt gctgatcgta gcgagccacg ttaaccggct gaccaaagaa
    16921 catcggttct ttcagctgat catttttcgt ctgtgaaaag gtggtatatg ccatgaatgt
    16981 gtcctgttgg gagtttaatg ccggataagg cattttacgc cgcatccggc atctcaatat
    17041 cagatcttac atgcgccgct ttcgcagcca tcgtcctgga ttgacggtac cagatcgtct
    17101 tgtgcgtctt cagcaccgtc acgggtgttc tgataataca gcgttttgac cccgaatttg
    17161 taggcggtga gcaggtcttt cagcagttgc tgcatcggca cttttcctga cgggaagcgt
    17221 gacggatcgt agttggtgtt agcagagatc gactgatcga taaatttctg catgataccc
    17281 accagttgca ggtagccatc gttacccggc atttcccaca gcagttcata ggcgtcgtgc
    17341 agatgctcgt agtccggcac cacctggcgc agaataccgt ctttcgacgc tttgatgctg
    17401 acgtaaccac gcggcggttc aataccgtta gtggcgttag agatctgcga agaggtctcg
    17461 gacggcatca gggcagaaag cgtggagtta cgcaggccgt gcgttttgat tgactcacgc
    17521 agtgcttccc agtcgtaatg cagcggctca ttagcgatgg catccagatc tttcttatag
    17581 gtatcgatcg gcaggatccc tttcgcgtaa gtggtttcgt taaaccacgg gcacgcgcct
    17641 tgctctttcg ccagctcatt agacgctttc agcaggtaat actgaatggc ttcgaaggtt
    17701 ttatgcgtca ggttgttggc gctgccgtcg gagtagcgtt taccgtgctt cgctaggtag
    17761 taagcgaagt tgatcacacc aatacccagc gtacgacgac ccatcgctcc acgtttggcg
    17821 gccgggatcg ggtaatcctg ataatccagc agcgcgtcga gtgcgcgtac tgccagaatt
    17881 gccagctctt ccagttcatc caggctgtta attgcgccca ggttgaaagc agacagcgta
    17941 cacagcgcga tttcaccgtt ctcgtcgttg acgtcgttca gtggtttggt cggtagggcg
    18001 atttccaggc acaggttaga ctggcgcact ggtgcaatag ccggatcaaa cgggctgtgg
    18061 gtgttgcagt ggtctacgtt ctgaatatag atacgaccgg tagacgcacg ttcctgcatc
    18121 atcagcgaga acagctcaac ggctttcaca cgctgcttgc ggatgctgtc gtctttctca
    18181 tatttggtgt acaggcgttc aaactcttcc tgatcggcga agaacgcgtc gtacagcccc
    18241 ggtacgtcgg acgggctgaa cagggtgata tcttcacctt tcagcagacg ggtatacatc
    18301 agtttgttga tttgtacccc gtagtccata tgacgcacgc ggttgccttc aaccccacgg
    18361 ttgtttttca acaccagcag gctttccact tccagatgcc acatcgggta gaacagtgtt
    18421 gccgcaccgc cgcgcacacc gccctgagag caggatttca ccgctgtctg gaaatgtttg
    18481 taaaacggaa tacagccggt gtggaatgct tcgccaccgc gaatcgggct acccaacgca
    18541 cgaatacgcc cggcgttgat gccgatcccg gcacgctggg aaacgtattt aacaatcgcg
    18601 ctggaggtgg cgttgatgga atccaggctg tcgccgcact cgatcagtac gcaggagctg
    18661 aactgacgag tcggggtacg cacgccggac atgattggcg tcggcagcga aattttaaat
    18721 gtggaaaccg cgtcgtaaaa acgcttcacg tattgcaggc gcgtttcacg cgggtagttc
    18781 gagaacaggc acgcggcaac cagaatataa aggaactggg cgctttcgta gatttcgccg
    18841 gtcacgcggt tctgtaccag atatttgcct tccagctgct taacggcagc ataagagaag
    18901 gtcatatcac ggtcgtgatc gataaacgtg tccatctgct tgaactcttc ttccgtgtag
    18961 tcttccagca gatgattatc gtatttgccc atctcgacca ttttcaccac gtggtcgtac
    19021 agcgcaggcg gctcaaactg gccgtaggct tttttacgca ggtggaagat cgccaggcgc
    19081 gcggcgagat actgataatc cggcgcatca cgggagatca ggtctgcggc agccttgata
    19141 atggtttcgt ggatgtcaga ggtcttgata ccgtcataaa actgaatgtg ggagcgcagt
    19201 tcgacctggg aaatcgaaac gttatgcagt ccttctgccg cccaatccag aacgcgatgg
    19261 attttgtcga gattgatgcg ctctgtgcta ccgtcgcgct ttgtcaccag cagattctga
    19321 ttcatgtatg tcgtacctgt ttttggaatt ctttccgcaa tagtttcatg cagactacct
    19381 gtagtgaggg aagctgctta gaacactata tatagggggt atgtttggga ttcactgcaa
    19441 gatagtgtga aaatggccct cttgcaagtg cataactttg tggataactc aggaaggaaa
    19501 aagtggcttt cgcgcacctt aggtcagaca aggtgtccgg gaaagtcaat gggaagagaa
    19561 aaatttgtta aaaataactg ttcggtgatt tcttgaacgg cagaataggt tggataaatt
    19621 gatatagatg gtttattaat ctttttgcta atgattgcaa aaattgatct aagcacgagt
    19681 catcacataa tctgatgtat cagttgtggt caatatataa gcgaatgtaa gcgattgttg
    19741 atgtcgaaat ggtt
//
LOCUS       DAZKGF010000092        19568 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZKGF010000092 DAZKGF010000000
VERSION     DAZKGF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.092"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            196..3459
                     /locus_tag="ACHOQV_003031"
     CDS             196..3459
                     /locus_tag="ACHOQV_003031"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFP9631370.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQAAELPPSYINPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEANIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVIGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDHIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            3674..4012
                     /gene="symE"
                     /locus_tag="ACHOQV_003032"
     CDS             3674..4012
                     /gene="symE"
                     /locus_tag="ACHOQV_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFP9631371.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVIEGCIVLTAQPPAAEESELMQSLRQVCKLSARKQRQVQDF
                     IGVISSKTPR"
     gene            complement(4054..4107)
                     /locus_tag="ACHOQV_003033"
     CDS             complement(4054..4107)
                     /locus_tag="ACHOQV_003033"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFP9631372.1"
                     /translation="MSDERLRDIGLRREDVE"
     gene            complement(4096..4251)
                     /locus_tag="ACHOQV_003034"
                     /pseudo
     CDS             complement(4096..4251)
                     /locus_tag="ACHOQV_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001684533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
     gene            4394..5806
                     /gene="yjiR"
                     /locus_tag="ACHOQV_003035"
     CDS             4394..5806
                     /gene="yjiR"
                     /locus_tag="ACHOQV_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9631373.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            complement(6048..6992)
                     /locus_tag="ACHOQV_003036"
     CDS             complement(6048..6992)
                     /locus_tag="ACHOQV_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP9631374.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFNEFISELTRRMPQQRERIMTIAERIHNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQRYTGLSAEQMQALEQPLPESKRDPWIEY"
     gene            7459..8691
                     /gene="mdtM"
                     /locus_tag="ACHOQV_003037"
                     /pseudo
     CDS             7459..8691
                     /gene="mdtM"
                     /locus_tag="ACHOQV_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
     gene            8732..10012
                     /gene="yjiN"
                     /locus_tag="ACHOQV_003038"
     CDS             8732..10012
                     /gene="yjiN"
                     /locus_tag="ACHOQV_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFP9631375.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDAQDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            10128..11279
                     /gene="yjiM"
                     /locus_tag="ACHOQV_003039"
     CDS             10128..11279
                     /gene="yjiM"
                     /locus_tag="ACHOQV_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFP9631376.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            11289..12056
                     /gene="yjiL"
                     /locus_tag="ACHOQV_003040"
     CDS             11289..12056
                     /gene="yjiL"
                     /locus_tag="ACHOQV_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFP9631377.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTSFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            12053..12310
                     /gene="ytiA"
                     /locus_tag="ACHOQV_003041"
     CDS             12053..12310
                     /gene="ytiA"
                     /locus_tag="ACHOQV_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFP9631378.1"
                     /translation="MKEHLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            12375..13235
                     /gene="yjiK"
                     /locus_tag="ACHOQV_003042"
     CDS             12375..13235
                     /gene="yjiK"
                     /locus_tag="ACHOQV_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP9631379.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            13303..14481
                     /gene="yjiJ"
                     /locus_tag="ACHOQV_003043"
     CDS             13303..14481
                     /gene="yjiJ"
                     /locus_tag="ACHOQV_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9631380.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLVGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMILIALTLLMPSKKHAIVPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(14494..15048)
                     /gene="kptA"
                     /locus_tag="ACHOQV_003044"
     CDS             complement(14494..15048)
                     /gene="kptA"
                     /locus_tag="ACHOQV_003044"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFP9631381.1"
                     /translation="MAKYNDKELAETSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDIVVATSDKKRFSYSSDGTCIRAVQGHSTSQVAIGFIEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            15298..15981
                     /gene="yjiH"
                     /locus_tag="ACHOQV_003045"
     CDS             15298..15981
                     /gene="yjiH"
                     /locus_tag="ACHOQV_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFP9631382.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGTGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            15978..16439
                     /gene="yjiG"
                     /locus_tag="ACHOQV_003046"
     CDS             15978..16439
                     /gene="yjiG"
                     /locus_tag="ACHOQV_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFP9631383.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            16452..17624
                     /gene="iadA"
                     /locus_tag="ACHOQV_003047"
     CDS             16452..17624
                     /gene="iadA"
                     /locus_tag="ACHOQV_003047"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9631384.1"
                     /translation="MIDYSAAGFTLLQGAHLYAPEDRGICDVLIANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQMLVKDYSFSLSDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            17689..18600
                     /gene="hypT"
                     /locus_tag="ACHOQV_003048"
                     /gene_synonym="qseD"
     CDS             17689..18600
                     /gene="hypT"
                     /locus_tag="ACHOQV_003048"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFP9631385.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNREELVIPIQAY
                     AYRMNTRMNPIAERFWRELRELEIEPG"
     gene            18607..18978
                     /locus_tag="ACHOQV_003049"
                     /pseudo
     CDS             18607..18978
                     /locus_tag="ACHOQV_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
     gene            complement(19026..19409)
                     /gene="iraD"
                     /locus_tag="ACHOQV_003050"
     CDS             complement(19026..19409)
                     /gene="iraD"
                     /locus_tag="ACHOQV_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFP9631386.1"
                     /translation="MMRQSVQTVLPESTGHNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHELAISLEMM
                     LYHDDEPLTFDLVWQKGNWHRTMPQ"
BASE COUNT         4664 a         4939 c         4936 g         5029 t
ORIGIN      
        1 ccctaacagc cctccgcaat accctgctcc ccaaactcat ctccggcgag ctatccgtgg
       61 aggatctccc ggatcacatc aaacaaaccg aagccgcata acgcattttg cccctgtaaa
      121 atcaggggct ttctggtaag gttttctact gatacgggaa tgcttaccag aaattagcca
      181 gggttggagc acgatatgag tctctctttc agtgaagcaa aattagaaca agcgatcatt
      241 gaactgttac aggatcaggg atatcaacac ctgatcggcg ataatgtccc acgttcaagt
      301 ctcgatcagg tcattatcga agacgatctc cgtcattatt tagcggcacg ctaccagcct
      361 gatggcatta ctgaagaaga gattcagcga ctgatcaaac agttcaccac gcttccggcc
      421 tccgatcttt atgaaagcaa caaaacattt tgtcgctggc tggcaaatgg ttttctgttc
      481 aaacgcgacg atcggcaaca aaaagatctc tatattgaat tgctcgacac ccggcatcta
      541 cctgccgcac tgcgccagat atttgacgcc gaagatgtcc cgttgcaaca ggctgcggaa
      601 ctcccgcctt cctatattaa cccgccgctt aaccggatta agattgttaa ccagcttaaa
      661 atctccggca aagataatca gactcgtatt cctgacggca ttctctatat caacggtctg
      721 ccactggtcg tctttgaatt taaaagcgcg gtgcgcgagc aggaagccaa tataggcgat
      781 gcctggaagc agctctgtaa acgctaccgc cgggatattc cgcaactgtt tatctacaac
      841 gcgctctgca ttattagcga tggcgtaaat aaccggatgg gcaacctgtt tgcaccctat
      901 gaatattttt actcatggcg caaagtcacc ggtaatgaaa accgtgaaca ggacggaatt
      961 ccatcattgc actcaatgat tcaggggctg tttcatccgg tacgtctgct ggatgtgatt
     1021 aaaaacttta tctgcttccc ggataaagcc aagcacgaag taaaaatttg ctgccgatat
     1081 ccgcagtact atgccgcccg caaactctat tacagcatca agcaagcgcg taaacctttc
     1141 ggtagcggta aaggcggtac ctactttggc gcaacgggct gcggtaaaag ctacaccatg
     1201 caatttttaa cccgcttgtt aatgaaaagc gtggagtttg ccagcccgac cattgttttg
     1261 atcaccgacc gcaccgatct ggacgatcag ctttctgcgc aaatgtgcaa cgccaaaaat
     1321 tacattggcg acgacaccat cgttcctgtt accagccgtg aagatttgcg taatcaactg
     1381 gcgggacgca atagtggcgg tgtattcctg acaacgatcc ataaattcac cgaagacacc
     1441 gaactccttt ctgaacgcag caatatcatt tgcatctcgg acgaagcaca tcgcagccag
     1501 gttaacctcg accagaaagt catcgtcgat aaagaaagcg gaaaagtgcg caaaacgtat
     1561 ggctttgcga aatacctgca cgattcactg ccaaatgcca cctatgttgg ctttaccggc
     1621 acaccgattg acgcgacgct cgatgtcttc ggtgaggtga tcgacagtta caccatgacc
     1681 gaatccgttc aggatgaaat cactgtacgc atcgtgtacg aaggccgtgc ggctaaagtg
     1741 atcctggact ccagcaaact ggaagaagtc gaaaagtatt acgaagagtg cgcaaacgca
     1801 ggcaccaatg agtggcaaat cgacgaaagc aagaaagcca cggctaccat gaatgcggtt
     1861 ctgggtgatg aagatcggtt aaaagccctc gcggaagatt ttgctaaaca ttatgaaaaa
     1921 cgcgtagccg aaggttccac cgtaataggc aaagccatgt ttgtttgtgc cagccgtgaa
     1981 attgcctggg atttctaccg ccagcttaaa gctattcgcc ctgcctggtt tgaagtgaag
     2041 caagcacctg atggcgtcgt cctgacggaa caggaacaaa aagagttacc gccttctgaa
     2101 atggtgaaaa tggtcatgac gcgcggtaaa gatgacgacg aggcgcttta tgatttactg
     2161 ggcacgaaag aatatcgtaa agagctggat aagcagttca aaaacgctaa atcgaatttc
     2221 aaaattgcca ttgtcgttga tatgtggctg accggttttg atgttcctga actggatacc
     2281 atctatattg ataagccctt acaaaaacat aaccttatcc agactatttc tcgcgttaac
     2341 cgtaagctgg aaggcaaaag caaagggtta gtggtggact acatcggcat taaaagtcag
     2401 atgaaccagg cactggcaat gtattcccgc attgatgcca ccaactttga agatattcag
     2461 caatcggtaa tagaagttaa aaaccatctc gatttgttgg ggcaagtctt ttacgaattt
     2521 gacagtcggg attattttag tggtgagcca caagcgcaat tatcctgcct caaccgcgcg
     2581 gcggaatttg ttctgcgtac ccagaaagtt gaacgtcgtt ttatgggact ggttaaacgc
     2641 atgaaagccg cctacgacgt ctgctgcggc agcgaagcat tatcacagac agaacgtgat
     2701 catattcact attatcttgc tgttcgttca attgttttca aactgacgaa aggtgacgca
     2761 ccggatgtca cccagatgaa tgcacgcgtt cgtgaaatga ttgcagaagc gctaaaagct
     2821 gatggcgtag aagaaattta ttttcttggc gataaaaaag cggaatccat cgatattttt
     2881 gacgaagatt atctggcgcg aattaacaag atcaaacttc cggcaacaaa gatccagtta
     2941 ttacaaaaat tactggaaaa agcgatcagc gacttcaaga aagtgaacca gttgcaaggg
     3001 attaacttca cccgccgctt ccaggcgatt atagatcgtt ataatgagcg gcgagaagat
     3061 gatgtactca acggtgaaga attcgataca ttcagtcagg aaatgaccga tattatctat
     3121 gatattaaaa ccgaaatggg cacctgggcc gatttaggca ttgatattga agaaaaagcc
     3181 tttttcgaca ttcttgctca tatgcgcgat aaatatcagt tcacctatga cgatgaaaaa
     3241 atgctgtcac tggcaaaaga gatgaaaagc gtggttgaca acacatcgaa atatcctgac
     3301 tggagtaaac gcgatgatat taaagcgaaa ctgaaagttg aacttattct gcttctacac
     3361 aagcataagt tcccgccagt agcgaatgat gatgtttata tgggtgtgct ggcacaggca
     3421 gagaatttta aagaacatca tatgagtgct ttaaattaat tatgaaggcc ggatacactt
     3481 tgttatccgg ccattctgaa ttaacgttaa tttctcttcc agatctttcc agtaacgctt
     3541 ttattatttt ctatttttag ctggactttc cctgtattta ctgatgatat atccaggtat
     3601 ttagcgcggt gcgaatgtgc gccaacacac ccgcaccgct catcacaatc cctatttcac
     3661 gagaatagta atcatgactg acacgcattc tattgcacaa ccgttcgaag cagaagtctc
     3721 cccggcaaat aaccgtcaat taaccgtgag ttatgcgagt cgctacccgg attacagccg
     3781 tattcccgcc atcaccctga aaggtcagtg gctggaaacc gccggttttg ccaccggcac
     3841 ggcggtagat gtcaaagtga tagaaggctg tattgtcctc accgcccaac cacccgccgc
     3901 cgaagagagc gaactgatgc agtcactgcg ccaggtgtgc aagctgtcgg cgcgtaaaca
     3961 aaggcaggtg caggatttta ttggggtgat tagtagcaaa acgccacgct aagtttctct
     4021 ccccttcccc atacgggaag ggaaattgcc ccctcactcc acatcctccc tgcgtaaccc
     4081 gatatccctc aaccgttcat cactcatctg ctgtacaccc gccgggcctg catttgccgc
     4141 cgccaacgcc tcaccgcctg tcagagttgc acaagtctga taaacggcgt tttggctttg
     4201 ttttcgtgaa attccatctg ctgcctcctc atctttcaga ggcttcatca tccgtcttct
     4261 ggcagttcac aatacagatg caaatattag ttttatttaa catacagaca ggttaaaaca
     4321 gcatctgcat ccggtttttt agcctcatct gtaccggttt ttctctctgt atggtccaaa
     4381 taaggaatac agcatgacgc gttatcaaca tctggcgact ctacttgccg agcggattga
     4441 gcaagggctg tatcgtcacg gggagaaatt gccgtcggtg cgcagcttaa gtcaggagca
     4501 cggcgtcagt attagcaccg tgcagcaggc gtaccagacg ctggagacga tgaagctcat
     4561 cactccgcag ccgcgttcgg gttattttgt cgcacaacgt aaagcccagc cgccagtacc
     4621 gccgatgacg cgtccggtgc agcgcccggt ggaaattacc cagtgggatc aagtgctgga
     4681 tatgctggag gcgcatagcg acagttccat tgttccgtta agtaaaagca cgccggatgt
     4741 cgaaacgccc agcctgaaac cgctgtggcg ggagctaagc cgggtggtgc agcataatct
     4801 gcaaaccgtt ctcggttatg acttgttagc cggtcagcga gtgttgcgag agcagattgc
     4861 ccgcctgatg ctcgacagcg gctcagtggt caccgctgat gacatcatta tcaccagcgg
     4921 ctgccataac tcgatgtcgc tggcgttaat ggcggtatgt aaaccgggcg atattgtcgc
     4981 agtcgaatcc ccctgttatt acggttcaat gcagatgctg cgcggtatgg gcgtgaaagt
     5041 gattgaaatc ccaaccgatc cagaaactgg catcagcgtt gaagcgctgg aactggcgct
     5101 ggaacagtgg ccgattaaag gcatcattct ggtgccaaac tgtaataatc cgctgggatt
     5161 tattatgccg gacgcacgca aacgggccgt tctctctctc gctcagcgtc atgatattgt
     5221 gatttttgaa gatgatgtct acggcgaact ggcgacggag tatccgcgcc cgcggaccat
     5281 tcattcctgg gatatcgacg ggcgagtgct gctgtgcagc tcgttcagta aaagtattgc
     5341 accaggcctg cgcgtgggtt gggtcgcacc ggggcgttat cacgataaac tgctgcatat
     5401 gaaatatgcc atcagcagct ttaatgtacc gtccacgcaa atggcggcgg caacgtttgt
     5461 tctggaaggt cactatcatc gccatatccg gcggatgcgg cagatctatc agcgcaattt
     5521 ggcgctttat acctgctgga tacgggaata ttttccctgc gaaatctgta ttacgcgccc
     5581 gaaaggcgga tttttactgt ggatagaatt gcctgaacag gtcgatatgg tttgcgttgc
     5641 gcggcagctg tgccgcatga aaatccaggt ggcggcaggc tcgattttct cagcttccgg
     5701 caaataccgt aattgtctgc gcatcaactg cgctttgccg ctcagcgaaa cctatcgcga
     5761 agcactgaag caaattggcg aggccgtgta tcaggcaatg gaatgaattc gaattagtgg
     5821 cgaggcccct gatttcctct cacgaatcgc ctgtgactcg ttttatcttt gcaggggcat
     5881 cgccacgctc cagcatggct taggcaaagt gtgctttgtt cataccggat gcggcgtgaa
     5941 cgccttatcc ggcttacaaa tcgcgcaaat tcaatagatt gcagaggtaa tgtaggtctg
     6001 ataagcgtag cacatcaggc agctttgcgt ttgtcatcag tctctgatta gtactcgatc
     6061 catggatcgc gcttgctttc aggcaagggt tgctctaatg cctgcatttg ctctgccgaa
     6121 agtccagtat atcgttgtat ccattcagga tccgcaccgt tctgcaaaag caatctaagg
     6181 aggcgttgtt ccccttcttc tttccccttt tccatcccta acagccatcc atcattatga
     6241 attcgctctg caatcgtcat tattcgctcc ctttgttgtg gcattcgacg ggtaagttca
     6301 ctgataaact cattaaaacg cgcttcatcg ccagtcagta aaatgtaatt taacagcgca
     6361 gttatctggc tgtcattagc acactctgta accagtaata ttaccagttg atcgataagc
     6421 cccatcagat cgcgctggcg aatatgcttt tggatcaact ccaacagggc gactctgcga
     6481 tgctgcacaa tctcgtcgtc tggcacgaca gtgacatcca ccagcgggaa cgctgcggta
     6541 taaagcttcc gtgcggtggt cgggtcagca aattcgtcca gccagcacag ggaccaggga
     6601 taaggactac ggctaccgtg ataaaacagc atcgggatga ccagcggtag cggtcggcgt
     6661 ttatcatgct cgatatggcg ctgcatcacc gccatggaat atcgcatcag gcgaaaggcc
     6721 atatggatat cctcgcggct ctgatgttca atcactacgt aaatataacc atcaccttca
     6781 cgggtcttta ccgaccacag aatatcggag tgtagcgccc gcaatttttc atcgacgaag
     6841 ctggcggatt ccagttttaa gctgtcgaga tcgcacagtt cacgtaaatc tttgggtaag
     6901 tggatctcca taaaatcccg cgcggtgtca gggtgcgtga gaaaggattt aaataatgcg
     6961 tcatgcggcg tactggtcgt gaagtttgtc atggcgatcc gtcacctgtt gataagatgg
     7021 cccgacttta gcgactaaca aaaccattct cacccggtaa tttcccattc cccgaggggc
     7081 tttccaggct atttttattc ttctgcaaac gatacatttt tccggaaaca ggctcgcatt
     7141 ttacatttcc tgtacccggc agatattccc ctttccaccg cactcttttg attaaccgca
     7201 aattttaatt acacttaagg tgtatatttt ctatgcaacc catcaattca agaggtgtaa
     7261 tgtgctgatg actatttgta atcgttatac attctgacct ggagtcagaa ggtatttctc
     7321 tgtctgtgta ttcacaggca gtgtggttga ttacatgaaa ttcagtacat ttatccttct
     7381 cgcctgcctt tcgtcattac cgacggtatt gaatttcgtt ttccccattg gggttctccg
     7441 gacaaggagt tgtttgttat gccacgtttt tttgcccgcc atgccgccac gctgtttttc
     7501 ccgatggcgt tgattttgta tgactttgct gcgtatctgt cgacggacct gatccagcca
     7561 gggatcatta atgtggtccg tgattttaat gccgatgtca gtctggcccc tgctgccgtc
     7621 agtctctatc ttgctggcgg tatggcgtta cagtggctgc tggggccgct ttccgacaga
     7681 attggccgca ggccggtgct gattaccggg gcgttaattt ttacccttgc ctgcgccgcg
     7741 acaatgttca ccacgtcaat gacacaattt cttatcgcgc gtgcaattca gggcaccagt
     7801 atctgtttta ttgccaccgt tggttatgtc acggtgcagg aggcgtttgg acagacaaaa
     7861 gggatcaagt tgatggcgat tatcacctcc atcgtactga ttgcgccgat tatcggcccg
     7921 ctttccggcg cagctctgat gcactttgtg cactggaaag tcctttttgc catcattgcg
     7981 gttatgggtt ttatctcatt tgttggctta ctgctggcga tgccagagac ggtgaagcgc
     8041 ggcgcggttc catttagcgc caaaagcgtc ttgcgcgatt ttcgtaatgt cttttgcaat
     8101 cggctgttcc tctttggcgc agcaaccatc tctttaagct atatcccgat gatgagttgg
     8161 gtggctgtct cgccggtgat cctgattgac gcaggcggct taacgacctc gcagttcgcc
     8221 tgaacacagg ttccggtgtt cggcgcggtg attgtcgcga atgccatcgt ggcgcgtttc
     8281 gttaaagatc cgaccgaacc gcgctttatc tggcgcgccg taccccttca actggttggc
     8341 ctcgcgctgt tgattatcgg caatctgctg tcaccgcacg tctggctgtg gtcggtgctg
     8401 ggcaccagtc tgtatgcttt cgggattggt ttgattttcc cgaccttatt ccgctttacg
     8461 ctgttttcca ataacttacc gaaagggacc gtctccgcat cgctgaatat ggtgatcctg
     8521 atggtgatgt ctgtctcggt cgaaatcggc cggtggctgt ggtttaacgg cggtcgcttg
     8581 ccgtttcatc tgttagccgt cgtggcgggc gttatcgtcg ttttcaccct ggcgggattg
     8641 ctcaatcgcg tgcgccaaca tcaggctgcc gagctggcgg aggagcggtg atttttgcgc
     8701 gatcccgccg tcaggctcta ttcttaacat tatgaataaa ctcattgaac tcagacgcgc
     8761 caaaatgttg gcgctctctt tactgcttat cgccgctgct acctttgtcg ttacgctgtt
     8821 tttgccgccc aatttttggg tgagcggcgt gaaggcgatt gctgaagcgg cgatggtcgg
     8881 cgcgctggcg gactggtttg cggtggtggc gctgtttcgc cgcgtgccga ttccgatcat
     8941 ttctcgtcat acggcgatta tcccgcgtaa taaagaccgg attggcgaaa atctcggtca
     9001 gttcgtgcag gaaaaatttc tcgataccca gtcgctggtg gcattgattc gacgtcacga
     9061 accggcgttg ttgattggca actggtttag tcagccggag aacgcccgcc gcgttggtca
     9121 gcatctgttg cagatcatga gcggttttct tgaactgacc gatgatgcgc gtattcagcg
     9181 cctgcttaag cgcgcggtcc accgggcgat tgataaggtt gatctttctg gcaccagtgc
     9241 gttgatgctg gagagtatga ccaaaaacga tcgtcatcag gtgctgctcg atacgctgat
     9301 cgcacagttg atcgcccttc tccagcgcga taaatcgcgc aagtttatcg cccagcaaat
     9361 tgttcgctgg ctggagagcg agcatccact gaaagccaaa attttgccca ctgaatggct
     9421 gggcgaacat agcgcggagt tggtttctga cgcggtgaat tctttgcttg atgatatcag
     9481 ccgcgatcgt gcgcatcaga tccgccatgc gtttgatcgc gccacctttg ccctgatcga
     9541 caagctgaaa aacgatcctg aaatggcagc gcgagccgat gccgtaaaaa gctatctgaa
     9601 agaagacgaa gcttttaacc gctatctcag tgaattgtgg ggggatttac gggaatggct
     9661 gaaagtggat atcaacagcg aagattctcg tgtgaaagaa cgtatcgcac gagcgggtca
     9721 atggtttggc gaaacgttaa ttgccgatga tgccttgcgg gcgtcgttaa atggtcatct
     9781 cgaacaagcc gcgcaccgcg tcgcgcctga gttttccgca ttcctgacgc gccacatcag
     9841 cgatacggta aaaagctggg atgcgcagga tatgtcgcgg caaatcgagt taaatatcgg
     9901 caaagatctg cagtttatcc gtgtcaacgg tacgctggtt ggcggttgta ttggacttat
     9961 tttgtatttg ctgtcgcagc tcccggcctt gttccccctc ggcaatttat agaaatccat
    10021 gaaaaactaa gtggctaacc gcgagagaga tcaaataaac cacactaaaa gaatggtaat
    10081 aatgtggcct cttttcatta tctcccgtgg tacggggaag gaaaatcatg tcacttgtca
    10141 ccgatctacc cgccattttc gatcagttct ctgaagctcg ccagaaaggc tttctcaccg
    10201 tcatggatct caaggagcgc ggcattccgc tggttggcac ctattgcacc tttatgccgc
    10261 aagagatccc gatggcagcc ggtgcggttg tggtttcgct atgttccacc tctgatgaaa
    10321 ccattgaaga agccgagaaa gatctgccgc gcaacctctg cccgctgatt aaaagcagct
    10381 acggcttcgg caaaaccgat aaatgcccct acttctactt ttctgatctg gtggtcggtg
    10441 aaaccacctg cgacggcaaa aagaaaatgt atgaatacat ggcggagttt aagcccgttc
    10501 atgtgatgca actgccaaac agcgttaagg acgatgcctc gcgtgcatta tggaaagccg
    10561 agatgctgcg cttgcaaaaa acggtagaag aacgttttgg gcacgagatt agcgaagatg
    10621 ctctgcgcga tgccattgcg ctgaaaaacc gcgaacgtcg cgcactggcc aacttttatc
    10681 atttggggca gttaaatcct ccggcgctta gcggcagcga cattctgaaa gtagtctacg
    10741 gcgcaacctt ccggttcgat aaagaggcgt tgatcaatga actggatgca atgaccgccc
    10801 gcgttcgtca gcagtgggaa gaaggccagc gactggaccc gcgcccacgt attttaatca
    10861 ccggctgccc aattggcggc gcagcagaga aagtggtgcg cgcgattgaa gagaatggcg
    10921 gctgggttgt cggttatgaa aactgcaccg gggcgaaagc caccgagcaa tgcgtggcgg
    10981 aaacgggtga tgtgtacgac gcgctggcgg ataaatatct ggcgattggc tgctcctgtg
    11041 tttcgccgaa cgatcagcgc ctgcaaatgc tcagccagat ggttgaagaa tatcaggtcg
    11101 atggcgtagt tgatgtgatt ttgcaggcgt gccataccta cgcggtggaa tcgctggcga
    11161 ttaaacgtca tgtgcgccag cagcacaaca ttccttatat cgctattgaa acagactact
    11221 ccacctcgga tgtcgggcag ctcagcaccc gtgtcgcggc ctttattgag atgctgtaag
    11281 gagtggcagt gacatattcg attggcattg attccggctc aaccgccacc aaagggatct
    11341 tactggctga cggcgtgatt acgcgccgtt tcctcgttcc aacatccttt cgcccggcaa
    11401 cagcaattac tgaagcctgg gaaactctgc gcgaagggtt agagacaacg ccgtttctga
    11461 cgctcaccgg ctacggacgg caactggtgg attttgccga taaacaggtg acggaaatct
    11521 cctgtcacgg gctgggcgca cggtttcttg cgccagcaac gcgcgcagta atcgacatcg
    11581 gtggtcagga cagcaaagtg attcagcttg atgatgacgg taacctgtgc gatttcctga
    11641 tgaatgacaa atgcgcggcg ggcaccgggc gtttcctgga ggtgatctcg cgcacgcttg
    11701 gcaccagcgt cgaacagctc gacagcatta ccgaaaatgt cacgccgcac gccatcacga
    11761 gtatgtgcac agtgtttgct gaatcagaag tgatcagcct gcgctctgcg ggcgtagcgc
    11821 cagaagcgat tctcgccgga gtgattaacg cgatggcgcg gcggagtgcc aatttcattg
    11881 ctcgtctctc ctgtgaagcg ccgattctgt ttactggtgg cgttagccac tgtcaaaggt
    11941 ttacccacat gctggaaagc catctgggaa tgccggtaca aactcaccct gatgcgcaat
    12001 tcgctggtgc aattggcgcg gcggtgatcg gtcagcggca gagaaaacgc gcatgaaaga
    12061 gcatctgttt ttatttcact ccacggtcgg cgtcatacaa acccgcaagg cgttacaggc
    12121 agcgggcatg acttttcgcg tcagcgatat tccccgtgat ttacgcggcg gctgcgggtt
    12181 atgcatttgg ctgacctgtc cgcctggcga ggaaattcaa tgggtgatcc ctgggcatac
    12241 ggaatcagtt tattgtcagc aggatggtgg ctggcgctgc attgcacatt acgggatttc
    12301 tccgcgttaa ggattcatta agatcgctac gtatacttct cgatatctta ttgatttgcc
    12361 ttaaagggat taccgtgaca aaaagtattt cattaagtaa acgcatcttc gttattgtga
    12421 ttctttttgt cattgtggca gtgtgtactt tcttcgtgca atcttgcgcc cgtaaaagca
    12481 atcatgcagc atcatttcag aattaccacg ccaccattga cggcaaagaa attgcaggca
    12541 taacgaataa tatctcttcg ctgacctggt cggcacaaag caatactctt tttagcacta
    12601 tcaacaaacc tgccgccatt gttgaaatga ccaccaatgg tgatttgatc cgcacaattc
    12661 ctctggattt tgttaaggat ctcgagacga tcgaatacat tggcgataat cagtttgtca
    12721 ttagcgatga gcgcgattac gccatttatg tgatttcact gaccccaaac tcggaagtaa
    12781 aaatcctcaa aaaaataaaa atcccgctgc aggaatcccc aaccaattgc ggttttgaag
    12841 gattggccta ttctcgccag gatcacactt tctggttttt taaagagaaa aacccgattg
    12901 aggtctacaa agttaacggg ctattaagta gcaatgaatt gcacatcagc aaagacaagg
    12961 cactacaacg gcaatttact ttagatgatg tgtccggcgc agaatttaac caacaaaaaa
    13021 atacgcttct ggtgctgtct catgaatctc gcgcactcca ggaagtaacg ctggttggcg
    13081 aggttatcgg tgaaatgtca ctaaccaaag gcagtcgagg tttgtcgcat aacatcaaac
    13141 aggccgaagg ggttgcgatg gatgcctctg gtaatattta tatcgttagc gaaccaaatc
    13201 gtttttatcg ctttacccct caatcatcgc attaacaggc tggcatgctt cacctttttg
    13261 ttattaactt gttaacaaca aaaatgacaa acaaggttaa acatgccttc gtccacgcat
    13321 cccgtagaac gcttttcttt cagcaccgcg cttttcggga tgctggttct gaccttaggt
    13381 atgggtttag gccgcttcct ttatacgcct atgttgcccg tcatgatggc ggaaggatcg
    13441 ttttcattta gccagctctc gtggattgcc agcggcaact atgcggggta tctggctggc
    13501 agtctgctat tttcttttgg cgcatttcac cagccatcgc gcctgcgccc atttctgttg
    13561 gcttctgccc tggcgagcgg gttgttgatc ctcgcaatgg catggttgcc gccatttatt
    13621 ctggtgttat tgattcgcgt cctggtgggt gtcgccagcg ccggaatgct gatttttggt
    13681 tcgacgctga ttatgcagca cacgcgccat ccttttgtgc tggcagcttt gttttctggc
    13741 gttggcattg gcatcgcact gggcaatgaa tatgttctgg caggcctgca ttttgacctc
    13801 tcctcgcaaa cgttatggca aggcgccggg gcgctttctg gcatgatact gattgcactt
    13861 acgcttttaa tgccctcgaa aaaacacgcc atcgtaccaa tgccattggc aaaaacggag
    13921 caacagataa tgagctggtg gttactggct attctgtacg gcctggcggg atttggttat
    13981 atcatcgtcg cgacttacct gccgctcatg gcgaaagatg caggctcacc attgttaacc
    14041 gcccatctct ggacgttagt cggcttatca attgtgcctg gttgctttgg ctggctatgg
    14101 gccgcaaaac gttggggggc tttgccctgc ctgacggcga atttgctggt gcaggctatc
    14161 tgtgtgctgc ttactctcgc cagcgactcg cctctcttgc ttatcatcag cagtcttggt
    14221 tttggcggta cctttatggg cacgacatct ctggtgatga ctatcgcccg ccaactgagc
    14281 gtaccgggaa atctcaatct tttaggcttt gtgacgctca tttacggcat tgggcaaatc
    14341 ctcggcccgg cgctgaccag tatgctcggc aatggaacat cggcgcttgc cagcgccact
    14401 ctctgcggcg cggcggcgtt gtttattgct gcgcttatca gcacagtgca attatttaag
    14461 ctacaagtgg tcacttcgtg agagaagttt cccttaccac tcaagaaact caaccgcaac
    14521 tgttgatgtc agccagaccc cgttttccgc ttgccaaaaa gggatgcctc gtttcgccat
    14581 ttcctgtgct ttgacggtta aaataactgg cgatccgtgt cttgctccca ctttgcgtgc
    14641 cgttgcttca tcagcagaga gatgcacgta atggcgctcc cctgcaatca gcccttgttt
    14701 ttttatctcg tcaagaaaac ggcttgccgt accatggtag agaaattgtg gcggtgtttt
    14761 ttcgatgaag ccaatggcga cctgcgaagt cgaatgcccc tgaacggcgc gaatacacgt
    14821 accatcactg gaataactaa agcgcttttt atcgctggta gcgacaacga tatccagtaa
    14881 tgcgcgggtt aaccgcttgc ccgctttttg ggcacagagt atgagcttat cgatatccgc
    14941 ccatccttca cggtccagca cgatgccaat cgcctctggt ttatgtcgga ggacaaaact
    15001 taaaaacttg ctcgtctctg ccagctcttt gtcgttgtat tttgccatct cgttctcgtc
    15061 cctggatttt ctgtttttat ttactgatgt cattgtcact gggtttatga attttctgca
    15121 actcaatcct gcgcagaacc tttgccacac agcgtcattt ttccgctatg caaaaaacgc
    15181 atagccagaa acatacttgc attttcccca tacccacatg gctgataagg tttttcagct
    15241 ccaggaattt tcctggctca agcaacacac aaacacaaaa aaacaatggg gattgttatg
    15301 acacaacaag gggatgctgt agccggggag ctggcgacag aaaaagtggg cattaaaggg
    15361 tatctggctt tttttctcac cataatcttt ttttccggtg ttttttccgg gacagacagt
    15421 tggtggcgcg tttttgattt ttccgtgctg aacggatcgt ttgggcaact gcctggtgcg
    15481 aatggcgcca ccacctcgtt tcgcggcgca ggtggcacag gagctaaaga tggctttctc
    15541 ttcgcgctgg aactggcacc gtcagtcatt ctctctctgg ggattatttc aattaccgat
    15601 ggtcttggcg gattacgcgc cgcgcagcag ttaatgacgc ccgtgctaaa accgttgttg
    15661 ggcattccag gcatctgttc actggcgctg attgctaacc tgcaaaacac cgatgccgcc
    15721 gcaggtatga ccaaagaact ggcgcaggaa ggggaaatta ccgaacgtga taaagtgata
    15781 ttcgccgcct atcaaaccag cggtagcgcc atcatcacca actacttctc ttccggcgtc
    15841 gcggtgtttg cctttctggg cacgtccgta atcgtacctt tagccgtcat cctggtgttt
    15901 aaatttgtcg gagccaacat tttgcgcgtc tggcttaact tcgaagaacg tcgcaaccca
    15961 acgcaaggag cgcaagcatg acaactcagg tccgcaaaaa cgtcatggat atgtttattg
    16021 atggcgcgcg ccgtggcttt actatcgcta ccaccaacct gctgccaaac gtggtgatgg
    16081 cctttgtcat catccaggcg ctgaaaatta ctggcctgct cgactgggtg ggacatatct
    16141 gtgaacccgt aatggcgctg tggggattgc ccggtgaagc ggcaaccgtc cttttagccg
    16201 cattgatgag catgggcggc gcagtcggtg ttgccgccag tctggcaacc gctggcgcat
    16261 taaccggcca cgatgtcacc gttttactgc ccgcgatgta tttaatgggc aacccggtgc
    16321 aaaatgtcgg tcgctgcctg ggaacagccg aagtgaatgc caaatattac ccgcatatca
    16381 ttacggtctg cgtgattaac gcattgctgt cgatctgggt tatgcagctt attgtttaac
    16441 aaggagttac catgattgat tattccgcag ccggttttac cctgctgcag ggagcgcatt
    16501 tgtatgcgcc ggaagatcgg ggaatttgcg atgtcctcat cgctaacggc aaaattattg
    16561 ccgttgccag caacatccct tctgacattg taccagactg cacggttgtc gatctcagcg
    16621 ggcagatcct ctgcccaggt tttattgatc aacacgtcca tttgattggt ggtggcggcg
    16681 aagcaggtcc cacgacacgc acgccggaag tggcgttaag tcgtctgacg gaagcgggcg
    16741 tcacgtcagt ggttggtctg ctgggtaccg actctatctc tcgccacccg gaatccctgc
    16801 tcgccaagac ccgtgcgctc aatgaagaag gcatcagcgc ctggatgctg accggcgctt
    16861 atcatgtccc ttcccgcacc attacaggtt ccgtggaaaa agacgtggcg attatcgatc
    16921 gcgtgattgg tgtgaaatgc gccatctctg atcaccgttc tgccgcacct gacgtttatc
    16981 acctggctaa tatggcggcg gaatcccgcg ttggcggttt gcttggcggt aaacctggcg
    17041 tcaccgtgtt ccacatgggc gacagcaaaa aggcgttaca gcctgtctat gacctgctgg
    17101 aaaactgcga tgtgccgatc agcaagctgc tgccgaccca cgttaaccgc aacgttccgt
    17161 tgtttgagca ggcgctggag ttcgcgcgca aaggcggcac catcgacatc accagtagca
    17221 ttgacgaacc ggtcgcccct gccgaaggta ttgcccgcgc cgttcaggcg ggtattccgc
    17281 tggcacgcgt caccctcagc tccgacggca acggtagcca gccgttcttt gatgacgaag
    17341 gaaatttaac ccatatcggt gttgcaggtt ttgaaacatt gctggaaacc gtgcaaatgt
    17401 tggtcaaaga ctatagcttc agccttagcg atgccctgcg cccgctcacc agtagcgtag
    17461 ccggtttcct taacctgacc gggaaaggcg aaattctgcc aggcaatgac gcagacttac
    17521 tggtcatgac gcctgaattg cgcattgagc aggtatacgc tcgcggcaaa ctgatggtca
    17581 aagacggcaa agcctgcgtg aaaggaacgt ttgaaacggc ataaccggtt gtcagccgcc
    17641 cgaatggacg tagaatgccc gcaagggcgg ctgacagagt ataacgtaat ggatgactgt
    17701 ggtgcggttt tgcataacat tgaaaccaaa tggctttatg attttctgac cctggaaaaa
    17761 tgtcgcaatt tttcccaggc ggcagtcagt cgcaacgtct cgcaaccggc attcagccgc
    17821 cgcatccgtg cactggaaca ggcgattggc gttgaattgt ttaaccgcca ggtgacgccg
    17881 ctgcaactct cggaacaagg gaaaatcttc cattcgcaga tccgccatct gttgcaacag
    17941 ttagaaagca acctggcaga gctgcgtggc ggcagcgatt acgcgcaacg taaaatcaag
    18001 atagccgctg cacactctct ttccctcggt ctgttaccgt ccattatcag ccagatgccg
    18061 ccgctcttta cctgggcaat tgaagccatt gatgtcgatg aagcggtcga taaactgcgc
    18121 gaagggcaaa gtgactgtat tttttccttt cacgacgaag atttgctgga agcgccgttt
    18181 gaccacattc gcttatttga atctcaatta ttccccgtct gcgccagtga cgaacacgga
    18241 gaagcactgt ttaacctcgc gcagccacac tttccgttac tgaattacag ccgcaactcc
    18301 tacatggggc gattgattaa tcgcaccctg acgcgccaca gtgagttgag tttcagcacc
    18361 ttttttgtct cttcgatgag cgagctatta aagcaggttg ccctcgacgg ctgtgggatt
    18421 gcctggctgc cggaatacgc tatccaacaa gaaattcgca gcgggcagct cgttgtactg
    18481 aatcgggaag aactggtgat cccgattcag gcctacgcat accggatgaa tacccgcatg
    18541 aatccaattg ccgaacgctt ctggcgtgaa ctgcgcgagc tggagattga acctggttga
    18601 aagttttctg cagaaaatct tccggcgctg ggaattatga ttgaggttcc tgccgcagtc
    18661 atgattgcag acaaattagc cagtgaagtc gatttctttt ccatcggcac caacgatatg
    18721 acgcaatata ttatggcggc agacagagga aatagcaccg tcgcgaaact ggtcgattat
    18781 tgtaatgacg cggtgattaa cgctattgct atggtgtgtc aggccggacg taataatgaa
    18841 attccggtca gtatgtgcgg cgaaatggct ggagatatcc agcaaacagc acgactgctc
    18901 accatgggga ttgataagct cagtgccagt ccatcccggc ttccggcctt gaaagcagca
    18961 atcagagcct cccactaatt gtgacctaac gcgatacgtc ttgcgcgtat cgcgtttccc
    19021 gtcatttact gcggcatcgt gcgatgccag ttccccttct gccagaccag gtcaaacgtt
    19081 agcggctcat catcgtggta cagcatcatc tccagactaa tggccaactc gtggcaatag
    19141 gcttctttca gattgacctg caatgccgca atacgtggct catgaaataa aagcgcggaa
    19201 cgtaactggc ggcaatacca actctggaga agcttgtcgc tctgattatg cccttgccac
    19261 tcaggcatgc ctgaaacaag taatattggt agcggtgaat ggggagaact aaaaagttga
    19321 aagagatccc ggcatacgga atcacgcagc gataacgtat tgtgtcctgt actttcaggt
    19381 aataccgtct gaactgattg gcgcatcatt ttgcgcactc ctgacgttta gtggcatctg
    19441 tatcctgccc tggctgccgg acaccactgg gttgatgctc atattccata atcattcctt
    19501 tatgctcatt gcgtccctgg cactttcata agggatttat aagatccgcc aaagataaaa
    19561 cactgtac
//
LOCUS       DAZKGF010000093        19529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZKGF010000093 DAZKGF010000000
VERSION     DAZKGF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.093"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(45..1364)
                     /gene="brnQ"
                     /locus_tag="ACHOQV_003051"
     CDS             complement(45..1364)
                     /gene="brnQ"
                     /locus_tag="ACHOQV_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFP9631387.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(1771..3066)
                     /gene="phoR"
                     /locus_tag="ACHOQV_003052"
     CDS             complement(1771..3066)
                     /gene="phoR"
                     /locus_tag="ACHOQV_003052"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFP9631388.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKVTRFSFVIPERLIAKNSD"
     gene            complement(3124..3813)
                     /gene="phoB"
                     /locus_tag="ACHOQV_003053"
     CDS             complement(3124..3813)
                     /gene="phoB"
                     /locus_tag="ACHOQV_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFP9631389.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            4003..5205
                     /gene="sbcD"
                     /locus_tag="ACHOQV_003054"
     CDS             4003..5205
                     /gene="sbcD"
                     /locus_tag="ACHOQV_003054"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFP9631390.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELEESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            5202..8345
                     /gene="sbcC"
                     /locus_tag="ACHOQV_003055"
     CDS             5202..8345
                     /gene="sbcC"
                     /locus_tag="ACHOQV_003055"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFP9631391.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISSMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIKEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSVREHLRQWQQQLTHA
                     EQKLNALTAITLTLTADEVATALAQHAEQRTLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQHRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTMEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            8471..9667
                     /gene="araJ"
                     /locus_tag="ACHOQV_003056"
     CDS             8471..9667
                     /gene="araJ"
                     /locus_tag="ACHOQV_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFP9631392.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGMVLGNVLSGRISGRYSPLRIAAVTD
                     FIIVLALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQ
                     IAFNLGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPV
                     LAKPLG"
     gene            complement(9806..10714)
                     /gene="mak"
                     /locus_tag="ACHOQV_003057"
     CDS             complement(9806..10714)
                     /gene="mak"
                     /locus_tag="ACHOQV_003057"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFP9631393.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            10839..11750
                     /gene="rdgC"
                     /locus_tag="ACHOQV_003058"
     CDS             10839..11750
                     /gene="rdgC"
                     /locus_tag="ACHOQV_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFP9631394.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(11831..11911)
                     /locus_tag="ACHOQV_003059"
     CDS             complement(11831..11911)
                     /locus_tag="ACHOQV_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631395.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(11908..12237)
                     /locus_tag="ACHOQV_003060"
                     /pseudo
     CDS             complement(11908..12237)
                     /locus_tag="ACHOQV_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(12394..12678)
                     /gene="ppnP"
                     /locus_tag="ACHOQV_003061"
     CDS             complement(12394..12678)
                     /gene="ppnP"
                     /locus_tag="ACHOQV_003061"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFP9631396.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSLFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(12750..13427)
                     /gene="aroM"
                     /locus_tag="ACHOQV_003062"
     CDS             complement(12750..13427)
                     /gene="aroM"
                     /locus_tag="ACHOQV_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFP9631397.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(13685..13876)
                     /gene="yaiA"
                     /locus_tag="ACHOQV_003063"
     CDS             complement(13685..13876)
                     /gene="yaiA"
                     /locus_tag="ACHOQV_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFP9631398.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(13926..14450)
                     /gene="aroL"
                     /locus_tag="ACHOQV_003064"
     CDS             complement(13926..14450)
                     /gene="aroL"
                     /locus_tag="ACHOQV_003064"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFP9631399.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(14633..15091)
                     /gene="yaiI"
                     /locus_tag="ACHOQV_003065"
     CDS             complement(14633..15091)
                     /gene="yaiI"
                     /locus_tag="ACHOQV_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP9631400.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            15211..16020
                     /gene="proC"
                     /locus_tag="ACHOQV_003066"
     CDS             15211..16020
                     /gene="proC"
                     /locus_tag="ACHOQV_003066"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP9631401.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(16037..17152)
                     /gene="adrA"
                     /locus_tag="ACHOQV_003067"
                     /gene_synonym="dgcC"
     CDS             complement(16037..17152)
                     /gene="adrA"
                     /locus_tag="ACHOQV_003067"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFP9631402.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNALPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(17254..17574)
                     /gene="psiF"
                     /locus_tag="ACHOQV_003068"
     CDS             complement(17254..17574)
                     /gene="psiF"
                     /locus_tag="ACHOQV_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFP9631403.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(17693..19108)
                     /gene="phoA"
                     /locus_tag="ACHOQV_003069"
     CDS             complement(17693..19108)
                     /gene="phoA"
                     /locus_tag="ACHOQV_003069"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFP9631404.1"
                     /translation="MKQSTIALALLPLLFSPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATVGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(19208..19468)
                     /gene="iraP"
                     /locus_tag="ACHOQV_003070"
     CDS             complement(19208..19468)
                     /gene="iraP"
                     /locus_tag="ACHOQV_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFP9631405.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
BASE COUNT         4856 a         5202 c         4908 g         4563 t
ORIGIN      
        1 cacggggcat cgcagccccg tggttaaaac aaatgttcag tgatttagtg agcgctggag
       61 gtcacctgac gacctgccgc gcgatcccag ataatggcca gaaccaccat caccactgtt
      121 ggcattaacc acgccagacc ttgttcggcc agcggtaaac gctgcgccca ggacggtaag
      181 atatcgctga atgcagatgc tttgatcccg tcgagaatac caaaaagcag gctgataaac
      241 atcggcggag caatcacgcg ggacgaatta tgccaccatg agcgtgtaaa acttaataca
      301 accagtgcga tacacggcgg ataaatggcg gtcagcaccg gtacagagat ctgaatcagc
      361 tgactcaagc cgaggttaga caccaccatc gagaagccgc cgaggataaa caccagcgta
      421 cgataagaga gcggtacgta ctgggcgaag aattctgcac aagcacaggt caggccaacc
      481 gccgtgacca ggcaggcgat gaagattaac gccgccagca ggaagctacc gccgccgcca
      541 aaggtatgct gaacgtaagc atgcaggatc gccgcaccgt ttgcagactg atcgaccagc
      601 gacgcgctgt ctgaacccag acggaacagc gccaggtaca gcagagtcag accaacaccc
      661 gccatcaggc cagcccagac ggtataacgg gtcagcagac gcgcttcggt aacgccacga
      721 gaacgcgccg cgttaacaat aacgatacca aacaccattg cgcccagcgt atccatggtc
      781 agatagccgt tgacgaagcc gttagaaaac gcagcgtttt gataagcctc agtagccgtg
      841 ctgatagaac ccgccggcca gataattgcg gcaacagaca ggatgaccag cgcgataatt
      901 ttcagcggcg caaggaagtt gcccacggta tccagcagct tgcccggata gagcgaaacc
      961 agaataacga tagcgaaata gaccaggctg taaataaaca gcggcagtgc ggaatcaccc
     1021 gtcagcggcg caatccccac ttcaaaagaa acggtagctg tacgcggcgt agcgaacagc
     1081 ggccccaccg ccaggtaaca caccgttgcc agcagtacgc cagcaacttt accgattggc
     1141 gtgctgaggc tgtcaacacc gccgccaact tttgccagcg ccaccaccgt taataccggc
     1201 aggccaacgg cagtaatgag gaagccgaat gccgcagtcc agacgtgttc gcctgcctgc
     1261 aagccgacca ttggagggaa aataatgtta cctgcgccga cgaacaacgc aaatgtcata
     1321 aagcccagag cgatgatatc gcgcgatctt aattgatggg tcataaatca tactgcctgt
     1381 ggatgtggtg ttgtaacgtt aaatttttgt gccaatccct ggggatgata cagcggtata
     1441 tttaaacaat tttagcggga aatactccca accaggcgcg gcgaagaata gtttttcgat
     1501 ttaccgcgtt aatgcagtca gtatgacagc acctgaagcg caatttaaac gcttataacg
     1561 ttttaaagca agaagggatc taaaaaccag aggtatatag cgataagaaa tcaccaacca
     1621 gtataaaaca ctatttcaat gaaaaagaca tggctaatca tgcgaacaaa aaatcaccaa
     1681 ccgttgtaaa atctaacgcc cgtatttcga ctgcaaagaa aataagccag catcggctgg
     1741 cttatggcaa taaaagatga caaaggcgaa ttaatcgctg tttttggcaa ttaaacgttc
     1801 cgggataaca aaactgaaac gtgtaacttt tcctactgta ctctcaatat tcaggcgact
     1861 ttcgtgatga ttcaccgcat gtttcacgat cgctaaccct aatccgctac caccggtttg
     1921 ccgggaacgc gctttatcaa cgcgataaaa acgctcggtc aggcgcggaa tatgctccgg
     1981 tgcaatgccc ggtccgttat cttcaacact aaattcggca ccgtgcggca ctcgctgcca
     2041 gcgtacggtg atatgcgtgc cttccggcgt atgattcacg gcgttataga ccaggttcga
     2101 aatcgcactg cgtagctgat cttcgttgcc tgacaccttg aggccgttat ctatctcaaa
     2161 ggtaaatgtc tgttttttct gactcagagt ctgagcctcg cgctcaacga cgcgcagcat
     2221 catcggcaca tcaacctttt cattgagcaa atgcgtcggt gcggcttcta ttttcgacag
     2281 cgtcagcaat tgcttcacca acccttccat ccgctgggtc tgctcgcgca tggtgtgcaa
     2341 cgctttttcg cgtaccgcgc cttccagcgg ctgctcatcc atcatctcca ggtaaccctg
     2401 taacacggtc aatggcgtac gtaactcatg gctcacgttg gcaaaaaagt tacgccgcgc
     2461 cccttccagt tgatgcattt gcgtgacatc acgcgccacc atcagcaact gtttgtgggt
     2521 ataaggcata acgcgaattt ccagatgccg cccggtgttg agcaccagat taagcgggcg
     2581 agaaaaatca cgcgttttca gatattgcgt aaactccggg taacgcagca ggttaaggat
     2641 gttctgcccg ttatcttccg gccagcgcaa accaagaatc tgttgcgcca gaccgttaca
     2701 ccagaaaata ccgccctctt ccgtggtcag cactactgcg tcgggcagcg actccgcgcc
     2761 actacgaaag cgtttaatca gattgcccag ttcgcggcgg cgttttttat ttcgcagctg
     2821 catctggtgt aagccgtata gtagcggttc ccagctacca cgccccggtg gcggggtcat
     2881 actgcgatcc acccacagcc accatgaaag gcgcaataaa ttccagaaat gccagataag
     2941 cagtcctgtt accgatgcca gcaaaaacca gggcaggtaa ccaaaaaatg cacccaggat
     3001 gaaagccggg aggcagcaaa gtagcagctc cagcaccagc cttttccacg acagccgttc
     3061 cagcacgtaa gatactccag ttaagaaatc ataagccctg ctctgcgtcc gatgagcaag
     3121 gcgttaaaag cgggttgaaa aacgatatcc tgtaccgcgc acggtctgca ccatgcggtc
     3181 atgcccgccg ggctccagtg ctttacgcag gcgacgaatg tggacatcga ccgtgcggtc
     3241 ttccacatac acgttagttc cccagacgtg gtttaacagc tgctcgcggc tgtacacgcg
     3301 ctcaggatgc gtcataaaaa agtgcagcag tttaaattct gtcggcccca tctccagtgg
     3361 ctcttcgccc gccatcaccc ggtgagatgt cgggtcgagg cttagcccct gcatctcgat
     3421 cacctcttcc accgccattg gcgaaatacg gcgcattacc gctttgattc gcgccaccag
     3481 ctccttcggc gaaaacggct tggtgatata gtcatccgcg ccggtttcaa ggccgcgcac
     3541 gcgatcttct tcttcccctc tggcggtcaa catcaccact ggaatatccc gggtcatcga
     3601 ctcgcgcttg aggtgtttga tgaactggat accggagccg ccaggtaaca tccagtcaag
     3661 gagaattaaa tccggccagg gttcattcag ttgattcaca gcactgtcat aatcttccgc
     3721 ttcgaccggc tgaaagccat tttgttcgag cacgaagcag accatttcgc gaattggagc
     3781 ttcatcttct acgaccagaa tacgtctcgc catgatttgc cctgttgtaa taaataggtt
     3841 gcgatcatta atgcgacgtc attatgcgtc agatttatga cagatttatg aaaagctcgt
     3901 cgcacatatc ttcaggttat tgatttccgt ggcgcagaaa aaagcaaatg gcacatctgt
     3961 ttgggtataa tcgcgcccat gctttttcgc cagggaaccg ttatgcgcat ccttcacacc
     4021 tcagactggc atctcggcca gaacttctac agtaaaagcc gcgaagccga acatcaggct
     4081 tttcttgact ggctgctgga gacagcacaa gcccatcagg tggatgcgat tattgttgcc
     4141 ggtgatgttt tcgataccgg ctcgccgccc agttacgccc gcacgttata caaccgtttt
     4201 gttgtcaatt tacagcaaac tggctgtcat ctggtggtac tggcaggaaa ccatgactcg
     4261 gtcgccacgc tgaatgaatc gcgcgatatc atggcgttcc tcaatacaac cgtggtcgcc
     4321 agcgccggac atgcgccgca aatcttgcct cgtcgcgacg ggacaccagg cgcagtactg
     4381 tgccccattc cgtttttacg cccacgtgac attattacca gccaagcggg gcttaacggt
     4441 attgaaaaac agcagcattt gctggcggct attactgatt attatcaaca acactatgcc
     4501 gatgcctgca aactgcgcgg cgatcagcct ctgcccatca tcgccacggg acatttaacg
     4561 accgtgggtg ccagtaaaag tgatgccgtg cgtgacatct atattggcac gctggacgcg
     4621 tttccggcac aaaactttcc accagccgac tacatcgcgc tcgggcatat tcaccgcgca
     4681 cagattattg gcggcatgga acatgttcga tattgcggtt cccccattcc actgagtttt
     4741 gatgaatgcg gtaagagtaa atatgtccat cttgtgacat tttcaaacgg caaattagag
     4801 agcgtggaaa acctgaacgt accggtaacg caacccatgg cggtgctgaa aggcgatctg
     4861 gcgtcgatta ccgaacagct ggaacagtgg cgcgatgtat cacaggagcc acctgtctgg
     4921 ctggatatcg aaatcactac tgatgagtat ctgcatgata ttcagcgcaa aatccaggca
     4981 ttaaccgaat cattgcctgt cgaagtattg ctggtacgcc ggagtcgtga acagcgcgag
     5041 cgtgtgttag ccagccaaca gcgtgaaacc ctcagcgaac tcagcgtcga agaggtgttc
     5101 aatcgccgtc tggcactgga agaactggag gaatcgcagc agcaacgtct gcagcatctt
     5161 ttcgccacga cgttgcatag cctcgccgga gaacacgaag catgaaaatt ctcagcctgc
     5221 gcctgaaaaa cctgaactca ttaaaaggcg aatggaagat tgatttcacc cgcgagccgt
     5281 tcgccagcaa cgggctgttt gccattaccg gcccaaccgg tgcggggaaa accaccctgc
     5341 tggacgccat ttgtctggcg ctgtatcacg aaactccgcg cctttctaac gtttcacaat
     5401 cgcaaaatga tctcatgacc cgcgataccg ccgaatgtct ggcggaggtg gagtttgaag
     5461 tgaaaggtga agcgtatcgc gcgttctgga gccagaatcg ggcgcgtaac cagcccgacg
     5521 gtaatttgca ggtgccacgc gtagagctgg cgcgctgcgc cgatggcaaa attctcgccg
     5581 acaaagtgaa agataagctg gaactgacgg cgacgttaac cggactggat tacgggcgct
     5641 tcacccgttc gatgctgctt tcgcaggggc aatttgctgc cttcctgaat gccaaaccca
     5701 aagaacgcgc ggaattgctc gaggagttaa ccggcactga aatctacgga caaatctcgt
     5761 cgatggtgtt tgagcagcac aaatcggccc gcactgagct agagaagctg caagcgcagg
     5821 ccagcggcgt cgcgttgctc acgccggaac aagtgcaatc gctgacagcg agtttgcagg
     5881 tacttactga cgaagaaaaa cagttactta ccgcgcagca gcaagaacaa caatcactaa
     5941 actggttaac gcgtctggac gaattgcagc aagaagccag ccgccgtcag caggccttgc
     6001 aacaggcgtt agccgaagaa gaaaaagcgc aacctcaact ggcggcgctc agtctggcac
     6061 aaccggcacg aaatcttcgt ccgcactggg aacgcatcgc agaacacagc gcggcgctgg
     6121 cgcatactcg ccagcagatt aaagaagtaa atactcgctt acagagcaca atggcgcttc
     6181 gggcgagcat tcgccaccac gcggcgaagc agtcagcaga attacagcag cagcaacaaa
     6241 gcctgaatgc ctggttacag gaacacgacc gcttccgtca gtggaacaac gaactggcgg
     6301 gttggcgtgc gcagttctcc caacaaacca gcgttcgcga gcatctgcgg caatggcagc
     6361 aacagttaac ccatgctgag caaaaactta atgcgcttac ggcgatcacg ttgacgttaa
     6421 ccgccgatga agttgctacc gccctggcgc aacatgctga gcaacgcacg ctgcgtcagc
     6481 gcctggtcgc gctgcatgga cagattgttc cccaacaaaa acgcctggcg caattacagg
     6541 tcgctatcca gaatgtcacg caagaacaga cgcaacgtaa cgccgcactt aacgaaatgc
     6601 gccagcgtta taaagaaaag acgcagcaac ttgccgatgt gaaaactatt tgcgagcagg
     6661 aagcgcgcat caaaacgctg gaagctcaac gtgcacagtt acaggcgggt cagccttgtc
     6721 cactttgtgg ttccaccagc cacccggcgg tcgaggcgta tcaggcgctg gagcctggcg
     6781 ttaatcagtc tcgattactg gcgctggaaa acgaagttaa aaagctcggt gaagaaggtg
     6841 cgacgctacg tgggcaactg gacgccttaa caaagcaact tcagcgcgat gaaaacgaag
     6901 cgcaaagcct ccgacaagat gagcaagcac ttactcaaca atggcaagcc gtcacggcca
     6961 gcctcaatat caccttgcag ccacaggacg atattcaacc gtggctggat gcacaagatg
     7021 agcacgaacg ccagctgcgg ttactcagcc aacgacatga attacaaggg cagattgccg
     7081 cgcataatca gcaaattatc cagtatcaac agcaaattga acaacgccag cagcagctct
     7141 taacggcatt ggcgggttat gcactgacat tgccacagga agatgaagaa gagagctggt
     7201 tggcgacgcg tcagcaagaa gcgcagagct ggcagcatcg ccagaacgaa ttaaccgcgc
     7261 tgcaaaaccg tatgcagcag ctgacgccga ttctggaaac gttgccgcaa agtgatgaac
     7321 tcccgcacag cgaagaaact gtggtgctgg aaaactggcg gcaggttcat gaacaatgtc
     7381 tcgcattaca cagccagcag cagacgttac agcaacagga cgttctggcg gcgcaaagtc
     7441 tgcaaaaagc ccaggcgcag tttgacaccg cgctacaggc cagcgtcttt gacgatcagc
     7501 aggcgttcct tgcggcgcta atggatgaac aaacactaac gcaactggaa cagctcaagc
     7561 agaatctgga aaaccagcgc cgtcaggcgc aaacgctggt cactcagaca gcagaaacgc
     7621 tggcacagca tcagcaacac cgacctgacg ggttggctct cactgtgacg atggagcaga
     7681 ttcagcaaga gttagcgcaa actcaccaaa agttgcgtga aaacaccacc agtcagggcg
     7741 agattcgcca gcagctgaag caggatgcag acaaccgtca gcaacaacaa accttaatgc
     7801 agcaaattgc tcaaatgacg cagcaggttg aggactgggg atatctgaat tcgctaatag
     7861 gttccaaaga gggcgataaa ttccgcaagt ttgcccaggg gctgacgctg gataatttag
     7921 tccatctcgc taatcagcaa cttacccggc tgcacgggcg ctatctgtta cagcgcaaag
     7981 ccagcgaggc gctggaagtc gaggttgttg atacctggca ggcagatgcg gtacgcgata
     8041 cccgtaccct ttccggcggc gaaagtttcc tcgtcagtct ggcgctggcg ctggcgcttt
     8101 cggatctggt cagccataaa acgcgtattg actcgctgtt ccttgatgaa ggttttggca
     8161 cgctggatag cgaaacgctg gataccgccc ttgatgcgct ggatgccctg aatgccagtg
     8221 gcaaaaccat cggtgtgatt agccacgttg aagcgatgaa agagcgtatt ccggtgcaga
     8281 tcaaagtgaa gaagatcaac ggcctgggct acagcaaact ggaaagtgcg tttgcagtga
     8341 aataactatt cagcaggata atgaatacag aggggcgaat tatctcttgg ccttgctggt
     8401 cgttatcctg caagctatca ctttattggc tacggtgatt ggtagccgtt ctggtggttg
     8461 tgatggtggt atgaaaaaag tcattttatc tttggctctg ggcacgtttg gtttggggat
     8521 ggccgaattt ggcattatgg gcgtgctcac ggagctggcg cataacgtag gaatttcgat
     8581 tcctgctgcc gggcatatga tctcgtatta tgcgctgggg gtggtggtcg gtgcgccaat
     8641 catcgcactc ttttccagcc gctactcact caaacatatc ttattgtttc tggtggcgtt
     8701 gtgcgtcatt ggcaacgcca tgttcacgct ctcttcgtct tacctgatgc tcgccattgg
     8761 tcggctggta tccggctttc cgcatggcgc attttttggc gtcggcgcga tcgtgttatc
     8821 aaaaattatc aaacccggaa aagtcaccgc cgccgtggcg gggatggttt ccgggatgac
     8881 agtcgccaat ttgctgggca ttccgctggg aacgtattta agtcaggaat ttagctggcg
     8941 ctacaccttt ttattgatcg ctgtttttaa tattgcggtg atggcatcgg tctatttttg
     9001 ggtgccggat attcgcgacg aagcgaaagg aaagctgcgc gaacaatttc actttttacg
     9061 cagcccggcc ccgtggttaa ttttcgccgc caccatgttt ggcaacgcag gtgtatttgc
     9121 ctggttcagc tacgtaaagc catacatgat gtttatttcc ggtttttcgg aaacggcgat
     9181 gacttttatt atgatgttag ttgggctagg gatggtgctg gggatggtgc tggggaatgt
     9241 gctaagtggc cggatttcag gacgttattc accactgcgc attgcggcag taactgactt
     9301 tattattgtg ctggcactgc tgatgctctt tttcttcggc ggcatgaaaa caacgtcgct
     9361 tatttttgca tttatttgtt gcgcgggatt atttgccctt tcagcaccac tgcaaatatt
     9421 gttactgcaa aatgccaaag gcggagagtt attaggtgcc gcaggtgggc aaatagcgtt
     9481 taacctcggt agcgccgtcg gcgcatattg cggtggtatg atgctgacgc tggggctggc
     9541 atataattac gtggcgctgc ctgccgccct gctttcgttt gctgcgatgt cgtcgttgct
     9601 gctgtatggt cgctataagc gccagcaagc ggcggatagt ccggtgctgg cgaaaccact
     9661 ggggtagatt actgtcaccg tgcgcgttgc ttattgccgg atgcggcgta aacgccttat
     9721 ccggcctacg ggctgctcat tttgtaggcc tgataagacg cggcaagcgt cgcatcaggc
     9781 atgatgcgct aattgcctgg tttttttact cttgtggcca taaccacgca gcgccgcgta
     9841 cgccgctgga atcaccgtgc ttcgccttac gcaccggcgt ttcacattcg ccgccgaaga
     9901 caaattgttt aatcaactgc ggaaccgttt gatataaacg gtctacattg ctcatcccgc
     9961 cccccaggac aatcacatcc ggatcgagaa tattcacgac atgtgccagc gattttgcca
    10021 gccgcagctc gtagcgacgc aatgccagtt ccgctaccgg atcgctttct tcaaccaggc
    10081 ggataatttc actgcctttc agcgcatgtc cgctcaaacg acgataatcc gtcgcaaatc
    10141 ccgtgcccga aataaaggtt tcaatacaac cttgtttacc gcaataacaa gggacttcct
    10201 cgcgataacg cagttcgtct tcgtccatcc acggtagcgg attgtgtccc cactcacctg
    10261 ccgtgccatt gccgccgata tgcgcccgcc cattgaatgc cacgcccgcg ccgcatcccg
    10321 tgccgataat cacggcaaat accgtctgcg ctcccgctgc cgcgccatct actgcttctg
    10381 aaaccgccag acagttggcg tcatttgcca gccgcacttc ccgctgcaac ctcgcgctta
    10441 agtctttatc gaatggctga ccgttgagcc aggttgaatt ggcattcttc accacgccgg
    10501 tgtagggcga aatcgaacca ggaatgccca tacctaccgt tccgcgctgc cctgttgcct
    10561 gctccgccat atcaaccaac gtggcgatcg tttcaatagt ctgccgataa tcatcacgcg
    10621 gcgtgggcag acgatggcgg tacaactgct cccctgcatc gcccagtgca atcacttcag
    10681 ttttggtgcc gcctaaatcg atacctatac gcacggtact ctccttattt ttttcaatat
    10741 caatagcgta gagacggaca actggattgg caatgcaagg ccgccgacaa ttcgttatca
    10801 tgcccgctaa atttaacgac aaggccgtgg aaattatcat gctgtggttc aaaaatttaa
    10861 tggtttaccg tcttagccgc gagatttcgt tgcgtgcaga agagatggaa aaacagctag
    10921 cctcgatggc atttacccca tgcggcagcc aggacatggc gaagatgggc tgggttcctc
    10981 cgatgggatc gcacagcgat gcgttaacgc acgttgccaa tggtcaaatt gttatctgcg
    11041 cgcgcaaaga agaaaaaatc ctcccgtctc cggtgattaa acaggcgctg gaagcgaaaa
    11101 tcgccaaact ggaagcggaa caggcgcgta agctgaagaa aaccgaaaaa gattcgctga
    11161 aagacgaagt gctgcactct ctgctaccgc gtgctttcag ccgttttagc cagacaatga
    11221 tgtggatcga cacggttaac ggtttgatta tggtggactg cgccagtgcc aaaaaagcgg
    11281 aagatacgct ggcactgctg cgtaaaagcc tggggtcgtt accggttgta ccattgagca
    11341 tggaaaaccc gattgaactg acgctgaccg aatgggttcg ctccggcagc gcggcacagg
    11401 gcttccagct gcttgatgaa gccgagctga aatcgttgct ggaagatggc ggagtgatcc
    11461 gcgcgaagaa acaagatctg accagcgaag agatcaccaa ccacattgaa gccggaaaag
    11521 tggtgactaa actggcgctc gactggcagc agcgcattca gtttgtgatg tgcgacgatg
    11581 gttcgctcaa gcgtctgaag ttctgcgacg agctgcgcga tcaaaacgaa gatatcgacc
    11641 gtgaagattt cgcccagcgc tttgatgccg atttcatcct gatgactggt gaactggcag
    11701 cgttaattca aaacctgatt gaaggattag gtggcgaagc acaacgttaa ttgctgattt
    11761 acctttaatg ccggatgcga cgcctgccgc gtcttatccg gcgtacgaag ccacaccagg
    11821 aatataatcg ttagctacag cgggcaataa ttcttagcgc agcaatatta tgcgttttat
    11881 gctgtaactt gctccagggg cgttgtgtca ttgtttttcc tcaagccggt gacgcgcttc
    11941 actgcgaatt tttcgcgttg ccccgttttt cactaaatca cgtaacagcg aaagatcggg
    12001 ttgttcgaca aataagagta atgatggctc ctcttttaac cacgccgttt tcacatcggc
    12061 agccagctgt gaattattaa tagccagcga ccgctcctga ggctcagtca atgtcgttaa
    12121 caacaccgga gtatgtgcgt tgtgcaatgc cgtttggcgc aaatgatcat tgcgactttg
    12181 cagcaatgca caccaccatt catcatctga tttatctttt aacgtcgcca gttccgcaat
    12241 agtatctgga gatcttcgct gatatttata tcaaccttga tgaattatga atatatcgga
    12301 aatcgaaaaa atcccagcaa aggaaatatt attatgggtt aaagagagga catttcagaa
    12361 aaatgccctc ccgttcaagg ggaaggcgag gaattacaga tagcggcaca gataagaggt
    12421 gggttcggca acttgcagat gaaactcgct gtgaccggga acattaaaca gcgaaccggc
    12481 ttcatacact tgccagtcgg tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac
    12541 cgtcatctct tcaggctcag cggtgctgaa ggtgtattca ccttcaacca taacacccac
    12601 gctggcgcga ccagtgctgc tgctggaaaa gccgatcgat ttcactttgc cggaaaagta
    12661 ctcattactt tgaagcataa actggccctg tctggtagtt gaacatctac ttcactatag
    12721 gggccagagg cgctggctgt cacgcaaaat tacatcagta attccgcagc cagccgtgca
    12781 atcaatacgt tagagagcaa gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga
    12841 cgctggttaa atcccaaaca atccagcatg atgacatctg caccttttgc cagtaattct
    12901 ttcccggcat caatgatttt ttgttccgaa tcatgaatgg gattacccaa tgaaaatact
    12961 ggcggtttct gcaaaatttg ccatttttgc gcctgaacgg tcagcaactc ctcaaccggt
    13021 acgataaccc ccacctgatg atcttcaaca atagaggaaa ccagcggtgg caatatccgc
    13081 gacggctcaa gaaagatcgt attacgcgcg gtcatactac taatgtttgc tgtactcatt
    13141 aatataatga cgtcgtaacc ctgattatcg agcacttcaa ccacaccttg caggtctcgc
    13201 tccactttgc gacgcgaaac atgggccaga tggttgtcat ttaataatgt gagaatggtg
    13261 tcttcgcctg cttctggcgc gtactccgcc atcacttctt cgcggcttaa cttccccagc
    13321 aggctatgat gggaaatatt atcttcgtca atgtattctg tcaggagcgg caaaacttcc
    13381 tgcataggta caatgccgat ggtcaggatc gccaacgacg cactcattgt tcttccacct
    13441 tacttttatt tcgtcgttaa ttaccgggca agtgttaagc accactgctg acacttactt
    13501 gtgctacaaa tgacaaaaag cgtagcagca gacctgcact gcataatgta aagattccaa
    13561 atgattccag taatggattg ttattgttta atattctaat tatttgaaaa acatgaattg
    13621 tgaaaaatgt gacgcagatc ccttgtggcg gcactcagaa tcccttccga gcagtctgat
    13681 gcggtcactc tttgtcaggg tcctcatagc gttttttcgc atccatcgct tcctgagcgg
    13741 tcggatgttc actgatcaac gagtctggtt taggatgatc ggctctgagt tggtaccagg
    13801 ttaccgtctg tcctggcttt cctttttcaa tcgtcactat atatgcttca cgaggataag
    13861 gcggtttcgt tggcatcgtt cttccttatt tcacgggatg aacgttaagt ataggcgctc
    13921 gaaaatcaac aattgatcgt ctgtgccaga gcgctgcgaa tttcagaaat cacctggctg
    13981 ggttcgtttg ttgcgtcgat gataatatgc gcaacttcgc gatatagcgc atcgcgttct
    14041 tccagcactt cctgaacttc ttcgctcagt ggttttcccg ttaaggttgg ccgtaaatct
    14101 tcttccggtg cagcttgcag tcggttaacc aggactgata ctggcgcaca caaataaacc
    14161 acgatcccgt tattttgcat gaagtgacga ttaaattccg tcagaataat gccgccgcct
    14221 gtagcgataa cggtggatgg cgcagttacc gcttccagcg ccgccgtttc tctggcgcgg
    14281 aatcccgccc actcttccct ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc
    14341 aaccactggt cggtatcgac aaaccgacgg ttaagcgaat cggcaagggc cattccgacc
    14401 gttgttttac cacagccccg aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt
    14461 ccccaatagg tcgcaatgcg gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa
    14521 actagtacaa tttcgattgt aaagaaaaaa ttccacttaa agtgaaaatc tcaatacatc
    14581 ccttactata ccaataaata ttcaagaatg aagtgtaaat aataaattac atttagccac
    14641 gactacgttg cacttccagc caccacttct ccagctccgc agcaaaagcc tggcggtcac
    14701 gttgtgaaag actatctggt cctccggtct ggatcccact ggcacgtaag gtatccataa
    14761 aatcgcgcat cgtcaggcgc tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcggat
    14821 taagcgccgc cgcgcctttc tcgatggctt cagcagccaa aggtatatct gcggtgatca
    14881 ccaaatcgcc cgcttcacac tgccgaacaa tttcgttatc agcaacgtcg aaacctgccg
    14941 cgacgcgcag cgtacgaata aatcgcgatg gcggcacgcg taaactctgg tttgctacca
    15001 gtaccagcgg catctgcata cgttccgccg cgcgatacaa aatctcttta attacattgg
    15061 gacacgcgtc ggcatccacc caaattgtca taaagtcatc ctttgttggg taatcctcta
    15121 ttgtgtcgcg cttttgcctt ccggcatagt tctgtttatg cttctgccag cgattatcaa
    15181 aacaatgaat ttcacggcag gagtgaggca atggaaaaga aaatcggttt tattggctgc
    15241 ggcaatatgg gaaaagccat tctcggcggt ctgattgcca gcggtcaggt gcttccaggg
    15301 caaatctggg tatatacccc ctccccggat aaagtcaccg ccctgcatga ccagttcggc
    15361 atcaacgccg cagaatcggc gcaagaagta gcgcaaatcg ccgacatcat ttttgccgcc
    15421 gttaaaccag gcatcatgat taaagtgctt agcgaaatca cctccagcct gaataaagac
    15481 tctctggtcg tttctattgc tgcaggtatc acgctcgacc agcttgcccg cgcgctgggc
    15541 catgaccgga aaattatccg cgccatgccg aacactcccg cgctggttaa tgccgggatg
    15601 acctccgtaa cgccaaacgc gctggtaacc ccagaagata ccgctgatgt gctgaatatt
    15661 ttccgctgct ttggcgaagc ggaagtgatt gctgagccga tgatccaccc ggtggtcggt
    15721 gtgagcggtt cttcgccagc ctacgtcttt atgtttatcg aagcgatggc cgacgccgcc
    15781 gtgctgggcg ggatgccacg cgcccaggcg tataaatttg ccgctcaggc ggtcatgggt
    15841 tccgcaaaaa tggtgctgga aacgggagaa catccgggaa cactgaaaga tatggtctgc
    15901 tcaccgggag gcaccaccat tgaggcggta cgcgtactgg aagagaaagg cttccgtgcc
    15961 gcagtgatcg aagcgatgac gaagtgtatg gaaaaatcag aaaaactcag caaatcctga
    16021 tggcttttgc cggacgtcag gccgccactt cggtgcggtt acgtccggct ttctttgctt
    16081 tgtagagcgc caaatctgcc gatttcaacc actcgcgata gtgactcatt tgtggattca
    16141 gcggcgcaac ccccacacta atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg
    16201 tgtttagccc ttcatgcacc cgtaacatgg cggtgatggc gctctctgct ggcgtaccgg
    16261 acatgattac tgcaaactca tcgccgccaa accgaccaat cacatcgcta ccgcgtaggg
    16321 taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc acccacatca tggccccagg
    16381 tatcgttgat gctcttgaaa tggtcgatat cgataatcag taacgttgca tcgcgattat
    16441 gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt cggttataca
    16501 cgcccgtcat accgtcgcgg gtactcatga cctgcaacct gcgtttatgt tccgccagtt
    16561 tagtcgccgt ctggtagctg acccagccaa acagcagagg ataaatgaca ataatgggaa
    16621 gggagagcca ccattccagc ggtgcgctat tgaacgacac ggtgatgccc gtcagctcga
    16681 gggtgacaag gcaggagacc accatcaaca ccagacccgc gacaaacaga cgggggccgc
    16741 ctgcccccat caaattcaga cacataatca tcaacatcgc ggtggaaggc agtgcgttta
    16801 cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag ttgtaaattt
    16861 cccggctaag cggatcgacg gccctgctcg ctatttgcca ggctaaatgc ggccagacga
    16921 acgcccagcc gaccaacaac agccaccacc agcccggcga cggatgcgaa accagcgttg
    16981 aagcaatcgg taagaacatg ccagccaggc caaccgcgcg gggtagtctg acgcgacggg
    17041 caaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt tcctccccat
    17101 gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac attcagaaac
    17161 aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc gggtgagcca
    17221 ttttcgtagc tcacccgtta gcactatcag gtatcaggcg gctttcttga ggcaggcgct
    17281 cataaactta ttacgatcat cccctttcag agattgttgc gttgcttgat tattacattc
    17341 gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca ggcgcagact tgctgttctt
    17401 caggcaatca ctcatgtagg tcttacgagc atcccctttc aacgcctgcg ccgtcgcctg
    17461 ctgattacag gaggtcatac gctgttgttg cggggttaag gttctctcgg cagcgccgac
    17521 ggtggttaaa aaaaccagtc cgaaaagcaa ggtaaccagt aatgttattt tcatcgcacc
    17581 atccctcttc atgttttaac catgagcgtt tgcgcccgtg atctgccatt aagtctggtt
    17641 gctaacagca aaaaaaccac ccgacagcga aaattcaccg ccgggcgcgg ttttatttca
    17701 gccccagggc ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt ccaacgacat
    17761 tggcggcatg tgggccatac gctgcaatac gcaactgact gccggtatgt tcttgtgaat
    17821 cctcttcgga gttcccgtaa ctcatcacca tcactgcgcc atctttggta tttagcgcct
    17881 gggtgaggcc cggcgctttg gtgtccggcg cgacaatctg gctggcgtgg gcgtgatcag
    17941 cggtgactat gaccagcgtg ttgccatcct ttttagcgaa ttccagcgca cgttgtacgg
    18001 cttcatcgag atcgaccgtc tcgccaattt gcccacaagg attcgcagcg tgatcctgtt
    18061 tatcgatgga tgcaccttca acttgcagga aaaaaccttt ctcattttta ctcaacaatt
    18121 caatggcttt gtcggtcatc tgcgccaggg tcggtacact gtcattacgt tgcggattag
    18181 gcgtacaggt aactgcgggc ttatcgatat tgccgtggta cgttgctttc ggtcctagcc
    18241 agcgcactgg catattgccg tcagcaaaca ggccaagcag gggtttttgc tgattcgctt
    18301 ccgtcaccgc atttagcgag gcagcatcac tcaccaactg ataaccacgc gcctgtgcct
    18361 gttcacgcag cgtttttccc tgccattcgc cgacggttgc cgtttcagca aaggtttttg
    18421 cgccgccgcc aagcgtaaca tcggcacgag cgttaagcag ctgttcggta atcgatcctt
    18481 ttccgccttt ttccagagcg ttacccggac atttttcact ggtcgcgctc ggaccgtagc
    18541 atttgcgcga ggtcacatgc gccaccagcg cggcgggcgt ggcatcctgc aactctgcgg
    18601 tagaaacgtt accggtcgcc agacctgcgg cttttgccat ttccagaatc gttgggtgat
    18661 ctttttcgtg aatatcgacg cccagcgcac cgttataggt tttgacacca gttgaccagg
    18721 cggttgctga tgcagccgag tcggtgacgt agtccggttt accggttttt ttattcagcg
    18781 catagtgagt gtattgcccg gtaagcggta atgcatcgat acctttaaaa aagccgcccg
    18841 caccttcggc ataattgcgt gcagcagtaa tttccgagtc ccccatccca tcgccaatca
    18901 gcaaaataat atttttggcg ggtttatcgc taagagaatc acgcagagcg gcggtctgat
    18961 cccccgttaa acggcgagca ccgccgggtg cagtaatatc gccctgagca gcccggtttt
    19021 ccagaacagg catttctggt gtccgggctt ttgtcacagg ggaaaacagt aacggtaaga
    19081 gtgccagtgc aatagtgctt tgtttcactt tattttctcc atgtacaaat acattaaaaa
    19141 taaaaacaaa gcgactataa gtctcggccg tgacagcttt atgacagctg ttgaaaagat
    19201 taacttttta ctgacgagga tgcttcaata acttctttac ataattgcgc agcagctccg
    19261 tatcgtcgtc aggaatgctg gcatcgggct ttacctcgta cagcgccccc tctacctgat
    19321 caatcaacct ctgttggtca ttttgcgcca tattgcgaag cattgcagtg acgataatct
    19381 ccaaagcttc tacctgcgcg cacagttctt tcgactcttc ttctttttgg gcaagcttaa
    19441 ataacaactc agcaatgaga tttttcatgt ctgtatttcc ttatccaaag tatggagaag
    19501 ttaacattat gtttcacaac ttcatagtg
//
LOCUS       DAZKGF010000094        18419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZKGF010000094 DAZKGF010000000
VERSION     DAZKGF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.094"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            29..595
                     /locus_tag="ACHOQV_003071"
     CDS             29..595
                     /locus_tag="ACHOQV_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9631406.1"
                     /translation="MSKFVKAAVATAMLMGALTSASVVAAGNNGTVRFYGTIEDSPCS
                     IVPDDHKLEVDMGSIGTGSLTGGKTTTPKDFQIRLQDCNFNTETTMTTTFTGNPYSGN
                     SKNYSLSNMDNGTEIPNVSLVIGDQRGTGYDLGAGIDQDIAMDSSTGKGKQKQTLNFK
                     AWLVGETAAVTPTPAPFETLTTFQITYL"
     gene            681..3320
                     /locus_tag="ACHOQV_003072"
     CDS             681..3320
                     /locus_tag="ACHOQV_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP9631407.1"
                     /translation="MPEHSFLRLRGLSWWIALAISGSPFNVLADDTIQFDARFLELKG
                     NTKIDLGRFSQKGYVEPGKYNLRVHVNNHPLPDEYDIYWYVAENDPNKSYACLPPELI
                     AQFGFKDDFAKSLQWGHDGQCLKTDQIGGMEIKGDLSQSALLVSVPQAYLEYTDDDWD
                     PPSRWDEGIPGLIADYSINAQTRHENGGDDTNDISGNGTVGVNVGPWRLRADWQSDYQ
                     HTRSNDDGDTDDSGTQKNWEWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFSYIGGS
                     ISTDDQMLPPNMRGYAPDISGVARTTAKVTVTQMGRVIYETQVPAGPFRIQDIGDSVS
                     GTLHVRIEEQNGQVQEYDVSTASMPFLTRPGQVRYKVTMGRPQNWDHQVAGSFFSGGE
                     ASWGIANGWSLYGGALADENYQSAALGLGRDLALFGALAFDVTHSRVQLDDNSVYGNK
                     TLDGNSYRVSYAKDFDELNSRVTFAGYRFSEKNYMTMSEYLDANDTDRARTGNDKEMY
                     TVTYNQNFRDARVSVYLNYSHHTYWDREDQTNYNMMLSHYFNLGSLRNLSVSLTGYRY
                     EYDKSADKGVYLSLSLPWGDNSTISYNGNYGSGADSSQASLYHRIDDASHYTLSAGTS
                     ENHTSLDGYYSHDSTLAKVDLSANYHEGQYTSAGVSLQGGVTLTAHGGALHRTQNMGG
                     TRLLIDADGVANVPVEGNGSAVYTNMFGKAVVADVNDYYRNQAYIDLNKLPENAEATQ
                     SVVQATLTEGAIGYRKFAVISGEKAMVVLRLQDGSHPPFGAEVKNDNQQQVGLVDDEG
                     NVYLAGVKPGEHMTVFWEGESHCDISLPDPLPNDLFNGLLLPCQQKGGSAPVITHDIQ
                     PVIQEQTQQVTPMEPPMSVSSNQ"
     gene            3340..4089
                     /locus_tag="ACHOQV_003073"
     CDS             3340..4089
                     /locus_tag="ACHOQV_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9631408.1"
                     /translation="MTKHLSVKTLATVLSLLAATVSFSALASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKQPYLAQSWIEDEKGVKISSPLAVLPPVQRIDAMMNGQVKIQGLPDIH
                     KLPADRESIFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALEKASMQNPWQHKVT
                     LTRNGQEFTVNNPTPYYVIFSNASAQKNGNPADGFSPIVMSPKTSAPLKVKMASVPVL
                     TYVNDYGARMPLFFTCSGNSCQVDEELSRKG"
     gene            4106..4612
                     /locus_tag="ACHOQV_003074"
     CDS             4106..4612
                     /locus_tag="ACHOQV_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9631409.1"
                     /translation="MKRSCSVWAGTWLLMCCIGSDFAVAGSKSVNLSLRVLVDAPPPC
                     TVNGATVEFGNVFINKIDGVGYKQPIDYSLVCNNLAMDDLRLQMRATTVVINGETVIS
                     TGISGFGIRVQKSSDHSTLDLTSGSWLPFNFSSGVPALEAVPVKQSGTTLSAAEFNAS
                     ATIVVDYQ"
     gene            4609..5079
                     /locus_tag="ACHOQV_003075"
     CDS             4609..5079
                     /locus_tag="ACHOQV_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9631410.1"
                     /translation="MKRISLLMLWGFSSMALSNVSFHGYLVQPPNCTISNGKTIEITF
                     QDVFVDDINGSNYEQTVPYSITCDTAVRDPSMEMKLSWSGTQSDFDDAAVSTDISGLA
                     IRLKQAGQSFKLNTALVVNETALPVLTAVPVKKSGVDLPEADFEAWATLQVDYQ"
     gene            5076..5600
                     /locus_tag="ACHOQV_003076"
     CDS             5076..5600
                     /locus_tag="ACHOQV_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9631411.1"
                     /translation="MKKKRALVFICSLMLLYSVTAPAVDNLHFTGNLLGKSCTPVING
                     SLLAEVHFPTIAASDLMNLGQSERVPLVFQLKDCKSSTLFSVRVTLTGTEDSELPGFL
                     AFDASSSASGAGIGIETAAGAAVPVNSTTGVTFPLNQGNNSLNFNAWLQAKGGRDVTS
                     GDFSATATATFEYF"
     gene            5602..6444
                     /locus_tag="ACHOQV_003077"
     CDS             5602..6444
                     /locus_tag="ACHOQV_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFP9631412.1"
                     /translation="MKKWTMIFASLVLLVMSASVPKSYAGDKLTSASFDSVKIYYGKG
                     KMSIHNTGGLYVTVLTPNEAAYGVYNDAIRKNEQASVISWTGSGPAPVLILTDYGNAD
                     SSNCPGINTATFQCAYETFKITVQSDSYGCPWLASFYTITDQPGFGRYTGPTNHATVC
                     PTIPVASYDISWSENYVSHNKALQLQSNGSTITTTLSTYLMEDGKLCDGSVFDSRGAY
                     CRVVSELLTFTSYGCDKSTVTVSPTRHPVTDKQLHDIVVNVNTSSRQPIDSTCRFQYV
                     LNEL"
     gene            complement(6560..7111)
                     /gene="smrB"
                     /locus_tag="ACHOQV_003078"
     CDS             complement(6560..7111)
                     /gene="smrB"
                     /locus_tag="ACHOQV_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFP9631413.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7277..8209
                     /gene="prmB"
                     /locus_tag="ACHOQV_003079"
     CDS             7277..8209
                     /gene="prmB"
                     /locus_tag="ACHOQV_003079"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9631414.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            8244..9329
                     /gene="aroC"
                     /locus_tag="ACHOQV_003080"
     CDS             8244..9329
                     /gene="aroC"
                     /locus_tag="ACHOQV_003080"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP9631415.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9332..10156
                     /gene="mepA"
                     /locus_tag="ACHOQV_003081"
     CDS             9332..10156
                     /gene="mepA"
                     /locus_tag="ACHOQV_003081"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFP9631416.1"
                     /translation="MNKTAIVLLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10156..10965
                     /locus_tag="ACHOQV_003082"
     CDS             10156..10965
                     /locus_tag="ACHOQV_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9631417.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            10965..11513
                     /gene="epmC"
                     /locus_tag="ACHOQV_003083"
     CDS             10965..11513
                     /gene="epmC"
                     /locus_tag="ACHOQV_003083"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFP9631418.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11547..11825
                     /locus_tag="ACHOQV_003084"
     CDS             11547..11825
                     /locus_tag="ACHOQV_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFP9631419.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQKASQQ"
     gene            complement(11881..13887)
                     /gene="mnmC"
                     /locus_tag="ACHOQV_003085"
     CDS             complement(11881..13887)
                     /gene="mnmC"
                     /locus_tag="ACHOQV_003085"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFP9631420.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHLHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGHVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGSTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RLYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVGANEVEGVTGVV
                     TNCGGITYPQGGWLCPAELTCNVLKLAQQQGLQIRYQHQLKDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSEKEARCGV
                     RCATRDHLPMVGNVPDYDVTLVEYASLAEQKDEAVSAPIYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSNEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            14046..15266
                     /gene="fabB"
                     /locus_tag="ACHOQV_003086"
     CDS             14046..15266
                     /gene="fabB"
                     /locus_tag="ACHOQV_003086"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFP9631421.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15531..16709
                     /locus_tag="ACHOQV_003087"
     CDS             15531..16709
                     /locus_tag="ACHOQV_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9631422.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16706..17701)
                     /gene="flk"
                     /locus_tag="ACHOQV_003088"
     CDS             complement(16706..17701)
                     /gene="flk"
                     /locus_tag="ACHOQV_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFP9631423.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(18026..18166)
                     /locus_tag="ACHOQV_003089"
     CDS             complement(18026..18166)
                     /locus_tag="ACHOQV_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9631424.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEFRLNIMWSEFAAFLQC
                     KP"
BASE COUNT         4398 a         4619 c         4874 g         4528 t
ORIGIN      
        1 ctaattattt atgatttaag gacaaataat gagtaagttt gtaaaagcag cagttgccac
       61 ggcaatgctc atgggggcgc tgacttcagc atcagtcgtt gccgcaggta acaacggtac
      121 agtgagattt tacggcacaa ttgaagactc tccgtgctcg attgttcctg acgatcataa
      181 actggaagtg gatatgggca gcattggtac aggttcttta accggtggca agactaccac
      241 acctaaagat ttccagattc gtttacagga ttgtaatttc aatactgaaa cgacaatgac
      301 cacgacattc actggtaatc cgtactccgg caactcaaaa aactattccc tgtctaacat
      361 ggataatgga actgaaattc caaatgtaag tctggtgatt ggcgaccaac gtggtactgg
      421 ttatgacctg ggggcaggca tcgatcagga catcgcgatg gacagttcaa caggaaaagg
      481 caaacagaaa caaacgttga actttaaagc ctggttagta ggtgaaactg cagcggtaac
      541 accaacccca gcgccgtttg aaacgctaac gacgttccag attacatatc tctaattatg
      601 ctgttccccc ataactgcaa tgttatgggg tttataaata aaagataact aaattataat
      661 aatcatggat ggatacgtag atgcctgaac attcttttct tcgtttacgg gggctttcat
      721 ggtggattgc tctggcaatt tccgggagtc cttttaatgt actggcagat gataccattc
      781 aatttgatgc tcgatttctg gagttaaaag gaaataccaa aattgatctg gggcgttttt
      841 ctcaaaaagg gtatgttgaa ccagggaaat acaatttacg tgttcatgta aataatcacc
      901 cattacctga tgaatacgat atctattggt atgtagctga aaacgatccg aataaatcat
      961 atgcctgctt accacctgaa ctgatagccc agtttggctt caaagatgat tttgcgaaga
     1021 gtttgcaatg gggtcatgat ggacaatgtc tgaaaactga ccaaatagga ggtatggaaa
     1081 ttaagggcga tttaagtcaa tctgcgctct tagtttctgt gccacaggca tatctcgaat
     1141 acactgacga tgactgggac ccaccttcgc gttgggatga ggggattcct gggcttattg
     1201 ccgattacag tatcaacgca caaacccgac atgaaaatgg gggggatgac actaacgaca
     1261 tcagcggtaa tggtacggtc ggcgttaacg tggggccgtg gcgcttacga gccgactggc
     1321 agagcgatta ccaacatacc cgcagtaacg atgacggcga cactgatgat agcggtacgc
     1381 aaaaaaactg ggagtggagc cgttactacg cctggcgcgc cttgccgtca ttaaaagcca
     1441 aactttcgct gggtgaagat tatctcaact ccgatatttt tgatggcttc agttacattg
     1501 gcggcagcat cagtaccgat gatcaaatgt taccgccaaa catgcgcggc tacgcacccg
     1561 atatttcagg tgttgcacgc actaccgcta aagtgaccgt aacccaaatg ggccgtgtca
     1621 tttacgaaac ccaagtgccc gcaggtccat tccgcattca ggatattggc gattccgtat
     1681 ctggcacgct gcatgtccgc attgaagaac aaaacggtca ggtacaggaa tatgacgtca
     1741 gcacggcgtc catgccgttt ctgacccgcc ccggtcaggt gcgttacaaa gtgacaatgg
     1801 ggcgtccaca aaactgggac catcaggttg caggcagctt cttctcgggc ggtgaggcgt
     1861 catgggggat tgcgaatggc tggtcgctct acggcggggc gttggccgat gaaaactacc
     1921 agtcggcagc gttaggcctc ggtcgtgacc tcgcgctgtt cggagcgcta gcgtttgatg
     1981 tcactcactc tcgtgttcaa ctcgacgata acagcgtgta tggcaacaaa acgctggacg
     2041 gcaactctta tcgcgtcagc tatgccaaag atttcgatga actcaacagt cgggtgacgt
     2101 ttgccggata tcgcttctct gaaaaaaact acatgaccat gagtgagtat ctggatgcga
     2161 acgacaccga ccgtgcgcgc acgggtaacg acaaggaaat gtacacggtc acttacaacc
     2221 agaactttag ggatgcgcgc gtttcggtct atctcaatta ttctcaccat acctattggg
     2281 atcgcgagga tcagaccaac tacaacatga tgctttctca ttatttcaat ttaggaagtc
     2341 ttcgcaacct gagcgtttct ctcaccggtt atcgctacga atatgataaa agtgccgaca
     2401 aaggtgttta tctgtccttg agtttgccat ggggcgacaa cagcaccatc agttacaacg
     2461 gcaattatgg cagtggtgcc gacagcagcc aggcgagttt gtatcaccgc atcgatgacg
     2521 ctagccacta tacgttgagc gccggaacca gtgaaaatca caccagtctt gatggttatt
     2581 acagtcatga cagtacgctg gcaaaagttg acctgagtgc aaactaccat gaggggcaat
     2641 acacatctgc gggggtctcc ttacagggcg gtgtaacact gactgctcac ggcggggcgt
     2701 tgcatcgtac gcaaaatatg ggaggcacac gtctgctaat cgacgccgat ggcgttgcta
     2761 atgtgccggt cgaaggtaat ggttccgccg tttataccaa catgttcggg aaagcggtgg
     2821 tggcagatgt taatgattac taccgtaacc aggcttatat cgatttgaac aaactaccgg
     2881 aaaacgccga agctacccaa tcggtggtcc aggcaacgct gacggaaggc gctattggct
     2941 accgtaaatt tgcggtgatc agcggtgaga aggcgatggt agtgctgcgc ttgcaggatg
     3001 gcagccatcc gccatttggg gcggaagtga aaaacgataa tcaacaacaa gttgggctgg
     3061 ttgatgacga aggtaatgtt tatcttgcag gcgttaagcc gggtgaacat atgaccgttt
     3121 tctgggaggg agaatctcac tgcgatatca gcctgccaga tccgttaccg aatgacctgt
     3181 tcaatggctt gttgttgcca tgccagcaaa aagggggaag tgctcccgtc ataacgcatg
     3241 atattcaacc tgtaattcag gagcagacac aacaggtgac accaatggaa ccgccgatgt
     3301 ctgtttcatc gaaccaataa cgtgattaag gaatgatcca tgactaaaca tctctctgtc
     3361 aaaacattgg caacggtact gtcgttactg gctgcaacgg taagtttttc tgctctggcg
     3421 tcagtaacgc ccgatcgtac tcgcctgatt tttaatgaaa gcgataaatc catcagcgtc
     3481 acgctgcgta ataacgaccc gaaacagcct tatctggcac aaagctggat cgaagatgaa
     3541 aagggcgtca agattagttc gccgctggca gtattgcccc ccgtacagcg cattgatgca
     3601 atgatgaatg gtcaggtgaa aattcaggga ttgccagata tccataaatt gcctgccgat
     3661 cgtgaatcga tattttattt caatgtgcgt gaaattccgc cgaaatcgaa caaagccaat
     3721 acgttacaaa ttgcgctgca gacacgcatc aaacttttct ggcgacctaa agccctggaa
     3781 aaagctagca tgcaaaaccc atggcagcat aaagtgacct taacccgtaa cggtcaggaa
     3841 tttaccgtca ataaccctac tccgtattac gtcattttca gtaatgccag cgcgcaaaaa
     3901 aatggcaatc cggcagacgg tttctcgccg atagtgatgt cgccaaaaac ctccgcaccg
     3961 ctcaaggtga agatggctag cgtgccggtg ctgacctatg tcaacgacta tggcgcacgg
     4021 atgccactct ttttcacctg tagcggcaac agttgtcagg ttgatgaaga gctgagccgc
     4081 aaaggttaat gacggggaag taagtatgaa aagatcttgt tcagtctggg cgggaacatg
     4141 gttactgatg tgttgtattg gtagcgactt tgcagttgca gggagtaaat cagtaaattt
     4201 gagtttacga gtgctcgttg atgcccctcc tccttgcacg gttaatggtg cgacggttga
     4261 gtttggtaat gtattcatca ataagatcga tggggtaggt tataaacaac ccattgatta
     4321 ctcgcttgtt tgtaacaacc tggcaatgga tgacctgcgt ttacagatgc gggcaacaac
     4381 ggttgtgatc aacggtgaaa cagttatcag cacaggtatt tcagggtttg gtattcgggt
     4441 tcagaaaagt agtgatcact caacactcga cttaacgtct ggttcatggt tgccgtttaa
     4501 tttttcctcg ggcgtccctg cactcgaagc agtgccggtg aaacagagtg gtacaacctt
     4561 atcggcagcg gaatttaacg ccagtgccac aattgtggtg gattaccaat gaaacggatc
     4621 tcacttctta tgctgtgggg ctttagctcg atggcactct caaatgtctc ttttcacggc
     4681 tatttagtgc agccacctaa ctgcactatt agcaacggta aaaccattga aataaccttt
     4741 caggatgttt ttgttgatga cattaatggc agtaactatg agcaaaccgt gccgtattcc
     4801 attacctgtg atacggcggt tcgcgaccca tcaatggaaa tgaaattgag ctggagcggt
     4861 acgcagagcg actttgacga tgccgcggtt agcaccgata tctctggcct ggcgattcgg
     4921 cttaaacagg cagggcagag ctttaagctc aatacggcac tggtggtgaa tgaaacggcc
     4981 ttaccggtgt taaccgcagt accggtcaaa aagagcggtg tcgatttgcc tgaggcagac
     5041 tttgaagcct gggccacgtt acaggtggat tatcaatgaa gaaaaagcgt gcgttggttt
     5101 ttatctgtag cctgatgctg ttgtacagcg ttactgcccc ggcggtcgac aacctgcact
     5161 ttaccggtaa tttgctcggt aaatcctgta cgccagtgat caacggtagc ctgttggcgg
     5221 aggtgcactt tcccaccatt gccgccagcg atttaatgaa cctcgggcag tcagagcgag
     5281 taccgttggt atttcaattg aaggattgca aaagctccac gctgtttagc gtcagggtaa
     5341 cgctgacggg aacggaagat agcgaactgc ccggatttct ggcctttgac gcctcatctt
     5401 ctgcatccgg agctgggatt ggtattgaaa cggcggcagg cgcggcggtg ccagtcaaca
     5461 gcacaacggg tgtcacgttc ccgctaaacc agggcaataa cagtctcaat tttaatgcct
     5521 ggctacaggc gaagggcggg cgggatgtga cgtccggtga tttcagcgct acggcgacgg
     5581 caacctttga gtatttttaa catgaaaaaa tggacaatga tttttgcgag cctggtgttg
     5641 ctggtgatga gtgcgagcgt tccaaagagc tatgccgggg ataaactcac cagcgcgagt
     5701 ttcgacagtg tgaagattta ttatgggaag gggaaaatgt ctattcataa caccggaggt
     5761 ttgtatgtca cggtgttaac tcccaacgag gcggcctatg gggtgtacaa cgatgccata
     5821 aggaagaacg agcaagccag cgttatttcg tggacggggt cgggaccagc gccagtgttg
     5881 atcttaactg actacggtaa tgctgatagc tcgaattgtc ctgggataaa tacggcgaca
     5941 tttcagtgtg catatgagac gtttaagata accgttcagt ccgacagtta tggttgcccg
     6001 tggctcgctt ctttttatac gatcacagat caacccggat tcgggagata tactgggcca
     6061 acgaaccatg ccacagtatg tccgacgatc cctgtcgcgt cgtatgacat ctcgtggagt
     6121 gagaattacg tcagccacaa taaggcgtta cagttgcaat ccaatggctc gacaatcacc
     6181 accacgcttt cgacctacct gatggaagat ggcaaactct gtgatggcag cgtttttgat
     6241 agcagaggtg catactgccg cgtggtctcg gaactgttaa cctttacctc ctacggttgc
     6301 gataaatcaa cggtaacggt ttctcccaca cggcatccgg taaccgataa acagctacat
     6361 gatatcgtgg taaatgtgaa caccagtagc cgacaaccga ttgattctac gtgccgattc
     6421 cagtatgtac tgaatgaatt gtagtgacga tattttcaca agtcgacggg gccatctggc
     6481 cctgtcgtca tttccggcgt ctgttttatg atatgatggc taaggcgaac tttccctcta
     6541 cagcattcga tgccagcatt cagggcaatt caggcggcag ccactcttcc acttcaatca
     6601 acaccaacaa agccgcatcg ccaccatact cttttggtgc ctggtgaaat gccattacat
     6661 gtggatgctg cgccagccac agcggcgttt gctgcttcaa aatatgcttc ccgtgaccat
     6721 gcatcacgca ggcacaaaac acatgttcac ggcggcaggc ggcaatcaac gcccccagtt
     6781 cctgtttggc ttgcaactgc gtcagaccgt gcaaatcaag aaacaactcc ggtgaataat
     6841 caccgcgccg cagtttcttc gcttcaaaat ggctgacatc cgggcgaaca tatttcaccg
     6901 gaccttcggt atttaataat ggctgaaact catcggaaaa ataatggctg gcatcggcct
     6961 gctcctgaat caaccgcttg accggtactt cgctgatttt tttacgctgc ggtcggtgga
     7021 caatcgtgtc ctgcttaatc ttgcgagtcc ccgccatcaa ctggcgaaac agcgcctggt
     7081 cctcctcgct aagtgttgtt ttctttttca tttaactgtc tctatttcta attttcctac
     7141 agtttacctg actcgtagca caatcgatcg ttttaaacgt atttttcctc actcaccgcg
     7201 cgtgaatgct gatttatcgt catcttcatg gcaaactagc cgccgaaatt attgcgatca
     7261 tgccctggag gaatacgtgg ataaaatttt cgttgatgaa gcagtaaatg agctgcaaac
     7321 cattcaggac atgttgcgct ggtcggtgag ccgcttcagc gcggcgaata tctggtacgg
     7381 ccacggcacc gataacccgt gggatgaagc cgtgcaactg gtgttgcctt cactctacct
     7441 gccgctggat attccggaag atatgcgcac cgcgcgtctg acttccagcg aaaaacaccg
     7501 tattgttgag cgcgtgatcc gccgcgtcaa cgaacgcatt ccggtggctt acctgaccaa
     7561 taaagcctgg ttctgtggcc atgaatttta cgtcgatgaa cgcgtgctgg tgccgcgctc
     7621 gccgattggt gaactgatca acaataaatt tgccggactc atcagcaagc aaccgcagca
     7681 cattttagat atgtgtaccg gtagcggctg catcgccatt gcctgtgctt atgccttccc
     7741 ggaagcggaa gtcgacgcgg tagacatctc tcccgacgcg ctggcggttg ctgaacagaa
     7801 catcgaagaa cacggtttga tccacaacgt cattccgatt cgttccgact tgttccgtga
     7861 tttgccgaaa gtgcagtacg acctgattgt caccaacccg ccgtatgtcg atgcggaaga
     7921 tatgtccgac ctgccaaacg aataccgcca cgagccggaa ctcggtctgg catctggcac
     7981 tgacggcctg aaactgacgc gtcgcattct tggtaacgcg gcagattacc tcgctgatga
     8041 tggcgtgctg atttgtgaag tgggcaacag catggtacat cttatggaac aatatccgga
     8101 tgttccgttc acctggctgg agtttgataa cggcggcgat ggtgtgttta tgctcactaa
     8161 agagcagctt attgccgcac gagaacattt cgcgatttat aaagattaag taaacacgca
     8221 aacacaacaa taacggagcc gtgatggctg gaaacacaat tggacaactc tttcgcgtaa
     8281 ccaccttcgg cgaatcgcac gggctggcgc tcggctgcat cgtcgatggt gttccgccag
     8341 gcattccgct gacggaagcg gacttgcaac atgaccttga tcgtcgtcga cctgggacat
     8401 cgcgctatac cacccagcgc cgcgagccgg atcaggtcaa aattctctcc ggtgtttttg
     8461 aaggtgttac tactggcacc agcatcggat tgttgatcga aaataccgac cagcgttctc
     8521 aggattatag tgcgattaag gacgttttcc gtccaggtca tgccgattac acctatgaac
     8581 aaaaatacgg cctgcgcgat tatcgcggcg gtggacgttc ttccgcccgc gaaaccgcta
     8641 tgcgcgttgc agcaggggcg attgccaaaa aatatctcgc cgagaaattt ggcattgaga
     8701 ttcgcggctg cctgacccag atgggtgata ttccgctgga aatcaaagac tggtcgcagg
     8761 tcgagcaaaa tccgtttttc tgcccggacc cggacaaaat cgacgcgtta gatgaactga
     8821 tgcgcgcgct gaaaaaagag ggtgactcca tcggcgcgaa agtcaccgtc gttgccagtg
     8881 gcgtacccgc cggacttggc gagccggttt ttgaccgcct ggatgccgac atcgcccatg
     8941 cgctaatgag cattaacgcg gtgaaaggtg tggaaattgg tgatggtttt gacgtggtag
     9001 cgctgcgtgg cagccagaac cgtgacgaaa tcaccaaaga cggtttccag agcaaccatg
     9061 cgggcggcat tcttggtggt atcagcagcg ggcagcaaat cattgcccat atggcgctga
     9121 aaccgacctc aagcattact gtgccggggc gcacgattaa ccgctttggc gaagaagttg
     9181 agatgatcac caaaggccgt cacgatccgt gtgttgggat ccgcgcggtg ccgatcgctg
     9241 aagcgatgct ggcgatcgtt ttaatggatc acctgttacg gcaacgggcg caaaatgctg
     9301 atgtgaagac tgatattcca cgctggtaaa aatgaacaaa actgcgattg tgctgctggc
     9361 cctgcttgcc agtagcgcca gcctggcagc gacgccgtgg caaaaaataa cccaacctgt
     9421 gccgggtagt gcacaatcga taggcagttt ttcaaatggc tgtatcgtcg gtgctgacac
     9481 gctgccgata cagtccgaac attatcaggt catgcgtacc gatcagcgtc gctatttcgg
     9541 acacccggat ctggtgatgt ttatccagcg tctgagtcgc caggtgagca atctgggtat
     9601 gggtacggtg ctgattggcg atatggggat gcctgctggt ggtcgtttca acggcggtca
     9661 cgccagccac cagactggac tggatgtcga tatcttcctg caactgccaa aaacacgctg
     9721 gacctccgcg cagctcttgc gcccgcaagc gttagattta gtttcccgcg acggtaaaca
     9781 cgttgtcccg acgctgtgga agccggaaat tttcagcttg attaaactcg ccgcgcagga
     9841 caaagacgtc acgcgcattt ttgttaatcc ggcgattaaa caacaacttt gccttgatgc
     9901 gggcaccgat cgcgactggt tacgcaaagt gcggccctgg ttccagcatc gcgcccatat
     9961 gcatgtacga ctacgttgtc ctgccgatag cctggagtgt gaagatcaac ctttaccgcc
    10021 accgggcgat ggttgcgggg cagaactgca aagctggttt gaaccaccaa aaccgggaac
    10081 aacaaagcct gagaagaaga caccgcctcc gttgccgcct tcctgccagg cgctactgga
    10141 tgagcacgtg atctaatgga aacgtttaat agcctgttta tggtttcccc gctgctgctg
    10201 ggagttctct tttttgtcgc catgctggcg ggatttatcg actcgattgc cggtggcggt
    10261 gggttactca ccattccggc attgatggcg gcggggatgt ctccggctaa cgcgctggca
    10321 accaataaac tgcaagcctg cggcggctct atttccgcca ctatctactt tattcgccgc
    10381 aaagtggtta gtttaagcga tcagaaactc aatatcgcca tgacctttgt cggctcaatg
    10441 agcggcgcat tactggtgca gtatgttcag gctgatgtct tacggcagat tctgcccatt
    10501 ctggtgattt gtatcggcct ctatttcttg ctgatgccaa agctgggtga agaagatcgc
    10561 cagcgccgga tgtatggact gccatttgcg ctggtggcgg ggggctgcgt cggtttttac
    10621 gatggattct ttgggccagc tgccggatcg ttttacgcgc tggccttcgt tacgctgtgc
    10681 ggattcaacc tcgccaaagc cacggctcac gccaaattac tcaacgcaac gtcaaacatt
    10741 ggcggtctgc tgctgtttat tctcggcggc aaagtgattt gggcgacggg ctttgtgatg
    10801 ctggtggggc agttcctcgg cgcgcgcatg ggttcaagac tggtgttgag caaaggacaa
    10861 aagctgatcc gcccgatgat cgttatcgtc tcggcggtga tgagtgcaaa attactttac
    10921 gatagccacg gacaggagat cctccactgg ttggggatga actaatgaac agtacacacc
    10981 actatgagca gttgattgag atttttaata gctgctttgc cgatgaattt aatacccgtc
    11041 tgattaaagg cgacgacgaa ccgatctatc ttcctgctga tgcggaagta ccgtataacc
    11101 gaatcgtctt tgctcatggc ttttatgcca gcgcaattca tgagatttcg cactggtgta
    11161 ttgccggaaa agcgcgccgt gagcaggttg atttcggcta ctggtattgc ccggacggtc
    11221 gcgatgccca gacgcaaagc cagtttgaag atgttgaagt gaagccgcag gcgctggact
    11281 ggttgttctg cgtggcggcg ggatatccgt ttaatgttag ctgcgacaat ctggaagggg
    11341 atttcgaacc ggaccgcgtg gtgtttcaac ggcgggtaca tgcgcaggta atggattatc
    11401 tggcaaacgg cattcccgaa cgtccggcac gcttcattaa agcactacaa aattattatc
    11461 acacgccgga acttacggcg gaacagttcc cgtggccgga agcgctcaac tgaagccagc
    11521 gccacgtttt acaatgagga aagaagatga tcgcggagtt tgaatcacgc attctggcat
    11581 taatcgacgg tatggttgac catgccagtg atgatgagtt gtttgccagt gggtatttgc
    11641 gtggccacct gacattagcc atcgcagaac tggaaagtgg tgatgaccac tccgcccagg
    11701 cggtacatac aaccgttagc cagagtctgg aaaaagcgat tggtgccggt gagttgtcgc
    11761 cgcgtgacca ggcgctggtg accgatatgt gggaaaacct gtttcagaag gcgtcacagc
    11821 agtaagtaat gtgccggata aggcgttcac gccatatccg gcaagcaatg ccagagctga
    11881 ttaccccgcc ttaaccgctt tacctttcaa caacttccgc acccataacc gattcgggtt
    11941 taacgccgcc agcgtactgg catccatcgg aattggttcg ttgctcatct gcgccgccag
    12001 aatctcggca cacaacgggg cagagcacaa accgcgagaa cctaaagcag ctaacataaa
    12061 aagatcgtca taaattggcg cgcttactgc ctcatctttc tgctccgcca gcgaagcata
    12121 ttccacgagc gttacgtcat aatcgggcac gttgcctacc attggcagat gatcgcgggt
    12181 ggcacaacgc acaccgcagc gcgcctcttt ctcactgaca tcaacctctt ttgcccactg
    12241 cgcctgcggg aaacaatcaa tcaaccgctg gcgattctgc tgctgatcgt cctcactgta
    12301 cgccgtatct tcgctgccgc gatgataact ggcaccaata caatgatgtt gattcgccgg
    12361 attttgtggc gtgagataac cgtcatagca cagcacctgc ttcagctccg ctagttccgg
    12421 cgttgtcgga atatggctaa cctgcccggc aaccgaatac actggcagag acgatgtttg
    12481 gctgaaacgg ctgatttgat gcccgttcgc cagtaccacc acgctatgcg ttgcttgctg
    12541 atctcctgca aaattcagca accaacagtc atccttacgg gataaatcct ttaactggtg
    12601 ttgataacga atctgtaaac cttgctgttg cgccagtttc agcacattac aggtcagttc
    12661 tgctggacac agccatccac cttgcggata tgtaataccg ccacaatttg ttactacacc
    12721 tgtaacgcct tcaacctcat tcgccccgac cgcaacagcc agttctgtgg gtaaatccat
    12781 cgacagcatc tgtgcgattt tatgctggct tttctcatcc cagcctaact gcgtgacgcc
    12841 gcaccagtca tgatcaaatt tgacgggcaa taaatcgtaa agccgacgag caaaagtaaa
    12901 cgcattagag aaaaagcggt tcaacgcctc atcgtgttta cttaagagcg gatacagcgc
    12961 cccctggcga ttgccggaag cacccagcgc gggggcttca tccgcgcaat aaagcgttac
    13021 ttgccagccg cgccgtaata acgccagcga caacaacgcg ctggcaatac caccgccgat
    13081 aatcgctact tcccgtttgc tgctgcccgt acgattaaac cacggcgtgg agcaggggag
    13141 gggtaatgtc tgttccatca ccccacaaag catttcccgt ttgcgcccaa agcccttacg
    13201 tttttgcatc gtgaatccgg cgtcctgcaa accgcggcgg acaaaaccgg cagacgtaaa
    13261 tgtcgccagc gtgctgcccg gacgcgccag tcgtgccatg gcgttaaaca gattttgcgt
    13321 ccacatatcc gggtttttcg ctggcgcaaa gccgtccaga aaccaggcgt ccactttttg
    13381 attcagcgag tcgtccagtt ggctggtcag ttcgtttata tcgccaaacc ataaatccag
    13441 tgtcacgtgg ccttcatcga gcaataaacg atggcaaccg ggcaagggca ttggccactg
    13501 cgcctgaagt tgttctgccc acggagccag ttccggccag tgtagatgcg ctaaggctaa
    13561 atccgcacgg gtgaggggaa atttctcaaa actaatgaaa tgtaagcgtt gtaattgcgc
    13621 ttgcggatgt gcttcgcgaa actgatcaaa tgcctgccat agtgtcagga agtttaatcc
    13681 ggtgccgaag ccgctctctg ctaccacaaa cagaggatgt ggatgctcag gaaagcgtgc
    13741 ctctaattgg ttacctccga gaaaaacata acgcgtctct tccagtccgt tgtcattgga
    13801 aaagtaaacg tcgtcaaaat ctcgggaaac aggtgtaccc tcagcattaa attcgaggtt
    13861 ggcaggttgt atggcgtagt gtttcacgta agttactcgt ctgacgggca gtctgacgat
    13921 cttaacgatg tgtgtcaggc tgcgcaaatt tctctattaa ttggctgatc ggacttgttc
    13981 ggcgtacaag tgtacgctat tgtgcattcg aaacttactc tatgtgcgac ttacagaggt
    14041 attgaatgaa acgtgcagtg attactggcc tgggcattgt ttccagcatc ggtaataacc
    14101 agcaggaagt cctggcatct ctgcgtgaag gacgttcagg gatcactttc tctcaggagc
    14161 tgaaggattc cggcatgcgt agccacgtct ggggcaacgt aaaactggat accactggcc
    14221 tcattgaccg caaagttgtg cgctttatga gcgacgcatc catttatgca ttcctttcta
    14281 tggagcaggc aattgcagat gctggcctct ctccggaagc ttaccagaat aacccgcgcg
    14341 ttggcctgat tgcaggttcc ggcggcggtt ccccgcgttt tcaggtgttc ggcgctgacg
    14401 caatgcgcgg cccgcgcggc ctgaaagcgg ttggcccgta tgtggtcacc aaagcgatgg
    14461 catccggcgt ttctgcctgt ctcgccacgc cgtttaaaat tcacggtgta aactactcca
    14521 tcagctccgc gtgtgcgacc tccgcacact gtatcggtaa cgcagtagag cagatccaac
    14581 tgggcaaaca ggacatcgtg tttgctggtg gcggcgaaga gctgtgctgg gaaatggctt
    14641 gtgaattcga cgcgatgggt gcgctgtcta ccaaatacaa cgacaccccg gaaaaagcct
    14701 cccgtactta tgacgctcac cgtgacggtt tcgttatcgc tggcggcggc ggtatggtgg
    14761 tggtcgaaga actggaacac gcgttggcac gtggtgctca tatctacgct gaaatcgttg
    14821 gctacggcgc aacgtctgat ggcgcagaca tggttgctcc gtctggcgaa ggcgcagtgc
    14881 gttgcatgaa gatggcgatg cacggtgttg ataccccaat cgactacctg aactcccacg
    14941 gtacttcgac tccggttggc gacgtgaaag agctggctgc tatccgtgaa gtgttcggcg
    15001 ataagagccc ggcgatttct gcaaccaaag caatgaccgg tcactctctg ggcgctgctg
    15061 gcgtacagga agctatctac tctctgctga tgctggaaca cggctttatc gccccgagca
    15121 tcaacattga agagctggac gagcaggctg cgggtctgaa catcgtgacc gaaacgaccg
    15181 atcgcgaact gaccaccgtt atgtctaaca gcttcggctt cggcggcacc aacgccacgc
    15241 tggtaatgcg caagctgaaa gattaattcg gagtaggtcg gataggacgc gtcagcgtcg
    15301 catccgacga tatgcggcaa tgctgcctcc ggtactaaag aataagggaa ccctatggtt
    15361 ccctttttca catcattgac aatcgccgcc tgtaccaggc aaacttcccg ctttgtcgat
    15421 ttccttctga aaagacgtac gcgttaattc ctgccaacgc accgtaaccc tgaaaccaga
    15481 gagatgagac ggggatactc ctcgccttgc gctgcattct ggagtaatgc atgactgctg
    15541 taagccaaac cgaaacacga tcttctgcca atttttcgct cttccgcatc gcttttgcgg
    15601 tttttctcac ctacatgacc gtagggttgc cgttgccggt tatcccgctg tttgttcatc
    15661 atgaactggg ctatggcaac actatggtcg gcattgccgt cgggattcag tttctggcta
    15721 cggtgctgac gcgtggttac gccgggcgac tggccgatca atatggcgca aaacgttcgg
    15781 cgcttcaggg gatgttagct tgtggtctgg cggggggcgc gttgctgctg gcggcgattt
    15841 tgcccgtccc cgcaccgttc aaatttgccc tgttggtcgt cgggcgtttg attcttggct
    15901 ttggtgaaag ccagttactg acgggcgctc tgacctgggg attaggcatc gttgggccaa
    15961 aacattccgg caaagtgatg tcatggaacg gaatggcgat ttacggtgcc ctcgctgttg
    16021 gtgcgccgct tggcctattg attcatagcc attacggttt tgccgcactg gcgctcacca
    16081 caatggcatt acccttactg gcgtgggcct gtaacggcac agtgcgcaaa gtaccggccc
    16141 tggcggggga acgtccatcg ctgtggagcg ttgtcgggct tatctggaaa ccagggttag
    16201 gtctggcact acaaggcgtt ggttttgcgg ttatcgggac tttcgtttcg ctctactttg
    16261 ccagcaaagg atgggcgatg gcgggcttta cgcttaccgc gtttggcggc gcatttgtcg
    16321 tgatgcgcgt catgtttggc tggatgccgg accgttttgg cggcgtgaaa gtggcgatcg
    16381 tctctctgct tgtagaaacg gtgggcttgt tgctgctctg gcaagcccca ggtgcatggg
    16441 tcgcattagc gggcgcggcg ttaaccggag ccggatgttc gcttatcttt cctgcgctgg
    16501 gcgtggaggt ggttaaacgc gtaccgtcac acgttcgcgg caccgcactg ggcggttacg
    16561 ccgcgtttca ggatatcgcc ctcggcgtct ccgggccgct tgcgggaatg ctggcgacca
    16621 cgtttggtta ctcttcggta tttcttgccg gggcgatctc tgcggtgctg ggaattattg
    16681 tcacgatact gtcatttcgt cggggttaac gaaccagcca gaccagcatc aggataatcg
    16741 cgactaacag tatccacagc gcgggacgcg ttgccatatt ttgcaacgtg tccatcatcg
    16801 gtggaaaagg attaagcagc ggttgcatat cacgcggatc aatcccttta acccgccgtt
    16861 gataaagctg attaagaata tcctcggcct gggcagaaga aagcgatgat tgcgcagaaa
    16921 ggccatattt ttgctggata tatgccgata acgccgccag ttcactggca tctaaaggtt
    16981 gtttcagcgc catctgtagt gattcaagcg tcggcgtatt ttgctggctt agcgtctgac
    17041 gcgcctgcag ccaggtcacc agatggttaa acagtttcgc tggaattaac tcgccatctt
    17101 tcaccccgga aagttccagc atcgattgcc agatctgttt gctgggttct cccgttgccg
    17161 ccgcaagttt ggtcaccagc tgtttgagcg cattgtgctc cgccggtaat aacggacggt
    17221 cggtcgcctc gcgctgctgc ggttgcggaa taaccatctt cccttcctgc aacagggtta
    17281 gaatggtttt taattgctcc ggtgagagct gattcagcgg cgtctgacca aagttatgac
    17341 ggatataatc cgtgaccgcc tgacgattat tccccagacg taaatactcc cctaattgcg
    17401 ctaaaagctg gcgggcagaa tggctttttt gcgcggccag cagacgttgc gccagattgt
    17461 gctcagcggc agggaagtga cgcgaaagca gcggtgaatc tcccgacaga ccaatatcgt
    17521 gcctgatacc cgcccacagt tctgctcttt gctgttgcgt cagtgaggtc actttcgtca
    17581 ttaagctttc cagcgaagta cgttgctgac tggataaagg ctgattgccc gcgccagacg
    17641 gcaggttatc tccctgacct ggtggttgcc caggaggagg gccggaaata ggttgtatca
    17701 ttacgtatcc ttatacctga aatcttcgca agtatgcctg gccgcgagat tatggcacac
    17761 ttgtccggtt aactctcgtc tcatacaggt aacacaaacg tgaaaatcct tgttgatgaa
    17821 aaatgaaaat tatttaacat tttgatataa aaggatttat atcaattgtt gtccacgcaa
    17881 tgaccacatt ttcgagatat agaaaaagcc cgcagttttt acgctgcggg ctttgttttt
    17941 ctttgtgcct tttgtgtgag ggtgcgactt tcgaccgaac ctttggcaac cggatagcgg
    18001 gggctttcac ccccgctgcg gtttcttacg gtttacactg caagaacgcc gcaaattctg
    18061 accacatgat gttaagtctg aactcacacg gagaacggtg tatcatccag acagtcaaca
    18121 acatggttaa gcataaaatg ctcacgatga tcgttttttg cggcatagcg cttgactcct
    18181 tttacaggga ggcgctaacc ttccacttgc ttagggtaga tggtcagggc ctcgggttaa
    18241 cattaaagtt gactccgggg cctttccaca tccggccttc aggtaatcct tccagccatc
    18301 agccgaaagg cacccgcgtg taatgtactg atttcccttt gtgtgcgcaa tgcttgaggg
    18361 ggacggtaaa aaaacgcccg catattgcgg gcaaatacgt ctgtaatcca atggagaaa
//
LOCUS       DAZKGF010000095        18383 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZKGF010000095 DAZKGF010000000
VERSION     DAZKGF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.095"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..596)
                     /gene="rsmG"
                     /locus_tag="ACHOQV_003090"
     CDS             complement(<1..596)
                     /gene="rsmG"
                     /locus_tag="ACHOQV_003090"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP9631425.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLHVPALDGERHL"
     gene            complement(660..2549)
                     /gene="mnmG"
                     /locus_tag="ACHOQV_003091"
     CDS             complement(660..2549)
                     /gene="mnmG"
                     /locus_tag="ACHOQV_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP9631426.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2928..3371)
                     /gene="mioC"
                     /locus_tag="ACHOQV_003092"
     CDS             complement(2928..3371)
                     /gene="mioC"
                     /locus_tag="ACHOQV_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFP9631427.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3461..3919)
                     /gene="asnC"
                     /locus_tag="ACHOQV_003093"
     CDS             complement(3461..3919)
                     /gene="asnC"
                     /locus_tag="ACHOQV_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFP9631428.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4071..5063
                     /gene="asnA"
                     /locus_tag="ACHOQV_003094"
     CDS             4071..5063
                     /gene="asnA"
                     /locus_tag="ACHOQV_003094"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFP9631429.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5068..6519)
                     /gene="viaA"
                     /locus_tag="ACHOQV_003095"
     CDS             complement(5068..6519)
                     /gene="viaA"
                     /locus_tag="ACHOQV_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFP9631430.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCMALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6513..8009)
                     /gene="ravA"
                     /locus_tag="ACHOQV_003096"
     CDS             complement(6513..8009)
                     /gene="ravA"
                     /locus_tag="ACHOQV_003096"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFP9631431.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8232..10100
                     /gene="kup"
                     /locus_tag="ACHOQV_003097"
     CDS             8232..10100
                     /gene="kup"
                     /locus_tag="ACHOQV_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFP9631432.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10267..10686
                     /gene="rbsD"
                     /locus_tag="ACHOQV_003098"
     CDS             10267..10686
                     /gene="rbsD"
                     /locus_tag="ACHOQV_003098"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP9631433.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPHLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10694..12199
                     /gene="rbsA"
                     /locus_tag="ACHOQV_003099"
     CDS             10694..12199
                     /gene="rbsA"
                     /locus_tag="ACHOQV_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFP9631434.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12204..13169
                     /gene="rbsC"
                     /locus_tag="ACHOQV_003100"
     CDS             12204..13169
                     /gene="rbsC"
                     /locus_tag="ACHOQV_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP9631435.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13194..14084
                     /gene="rbsB"
                     /locus_tag="ACHOQV_003101"
     CDS             13194..14084
                     /gene="rbsB"
                     /locus_tag="ACHOQV_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFP9631436.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14210..15139
                     /gene="rbsK"
                     /locus_tag="ACHOQV_003102"
     CDS             14210..15139
                     /gene="rbsK"
                     /locus_tag="ACHOQV_003102"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9631437.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15143..16135
                     /gene="rbsR"
                     /locus_tag="ACHOQV_003103"
     CDS             15143..16135
                     /gene="rbsR"
                     /locus_tag="ACHOQV_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFP9631438.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLLHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16101..17528)
                     /gene="mdtD"
                     /locus_tag="ACHOQV_003104"
     CDS             complement(16101..17528)
                     /gene="mdtD"
                     /locus_tag="ACHOQV_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFP9631439.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17551..18243)
                     /gene="yieP"
                     /locus_tag="ACHOQV_003105"
     CDS             complement(17551..18243)
                     /gene="yieP"
                     /locus_tag="ACHOQV_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP9631440.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAAMVGTAEQKAHLNTLMTEMVALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4423 a         4566 c         4809 g         4585 t
ORIGIN      
        1 agatgacgtt cgccatccag ggccggaacg tgaagtttaa ccactgattc gacctgatat
       61 tcttcgggca acaaagcgat ttcatcttcc ggcatttggc ctttcagcgc gtagaaacgg
      121 ccttgctcac caggaagatg gtgacaccag ctcaccatat cgttcagaga ggcaaaagcg
      181 cggctaatta cgccatcaaa tggcggctct gaaggaaact cttctaccct gctctgtact
      241 ggctcaatat tctccagttt aagctcatgt tgcacctgac gaaggaaacg cacgcgttta
      301 ccaaggctat ccaacagagt gaaatgggct tcaggacgca cgatagagag tggaatgcca
      361 ggcagtcctg gaccggtgcc gacatcgata aaccgttcac cttgcagata cggtgccacc
      421 acaatgctat cgagaatatg gcgtaccagc atctcattag gatcgcggac cgaagtcagg
      481 ttgtacgctt tattccattt atgcagcata ttcacatagg caataagctg gtttttctgg
      541 tgatcggtaa gcgaaatacc tgcgtctttc agcagtaagg agagtttgtt gagcacggtg
      601 attacctgtt cttgatgcgt tgcccggtaa gcgggtgctt accaggcatt tttaatgcgt
      661 tatgcgctac gacgcagcat accctgtttt ttcagccaca ccagcagaat ggagatggcc
      721 gcaggcgtga cgccagaaat acgcgaagcc tggccgatag aggccggttt gtgatcgtta
      781 agtttggcga tcacttcgtt agaaagaccg gatacctggc ggtaatccag cgtcgctggt
      841 agcagggtgt tctcgttacg cagctgcttt tcgatctcat cttgctggcg cgcgatataa
      901 ccttcgtatt taacctgaat ctcaacctgt tccgccgcct gttcgtctgt caacgcaggg
      961 gcaaacggcg tcagcgtggt taatttttca taagtcattt ccggacgacg cagcagatct
     1021 tcaccactgg cttcacggga aagtggcgca gtcaggtgag cattcacttc ggctgcagct
     1081 tccgccgacg gggttaccca ggtcgatttc agacgctgac gctcacgctc gatattctca
     1141 agtttctcgt taaagcgcgc ccaacgttcg tcatccacca ggcccagttc acgaccaatt
     1201 tcagtcaaac gcagatccgc attatcttcg cgcagcatca gacgatattc tgcgcgcgag
     1261 gtaaacatac gatacggttc tttggttcct aaagtgcaca ggtcatcaac cagtacgccg
     1321 agatacgcct gagaacgcgc cggagcccaa ccttctttgt cagcagacag acgggcagcg
     1381 ttaagaccgg ccagcaaacc ttgcgcagcg gcttcttcgt aaccggtagt gccgttaatc
     1441 tgaccagcaa agaacagccc ctggataaac ttgctctcca gcgtcggttt caggtcgcgt
     1501 ggatcgaaga agtcatactc aatggcataa cccggacgca cgatcttcgc gttttccatc
     1561 ccctgcatgg agcggacgat ttgcatctgc acatcgaacg gcaggctggt ggagataccg
     1621 ttcggataaa tttcgtttga ggtcagccct tccggttcaa ggaagatctg atgctgattt
     1681 ctgtcggcga agcgcatgac tttgtcttcg atcgacgggc agtagcgtgg gccgacacct
     1741 tcgatcaccc ctgcatacat tgggctacga tcgaggttac tgcggatcac atcatgggtt
     1801 ttctcgttgg tatgggtgat ataacacggc acctgctgtg gatgctggga cgcattaccc
     1861 ataaacgaga ataccggcat tgggttatcg ccatgctgtt gcgccagcac gctgaagtcg
     1921 atagtacgcg catcaatacg cggcggtgtc ccggttttca gacgaccaac gcgcagcggc
     1981 agttcacgca aacggcgaga gagcggaatg gacggcggat caccagcacg accaccgctg
     2041 tagttatcca gaccgatatg aattttaccg tcgaggaacg tcccaacggt aagcacgacg
     2101 gctttggcac ggaacttcag tcccatttgg gtaacagcac cgaccacgcg atcgttttcg
     2161 acaataagat cttcaaccgc ctgctggaag atcatcaggt tcggttggtt ctccagcgcc
     2221 gtacgtaccg cctgacggta gagcacacga tccgcctgag ctcgggtagc gcgaaccgcc
     2281 ggtcccttgc ttgcgtttag tatcctaaac tggatacccg cctgatcgat cgctttcgcc
     2341 atcagaccgc cgagtgcatc cacttctttt accagatgtc ccttcccaat accgccgatc
     2401 gccgggttgc agctcatctg ccccagagtg tcgatattgt gtgtcaaaag cagagtctgt
     2461 tgacccatac gcgccgcggc catcgcggcc tcggtgcctg catgaccccc gccaatgatg
     2521 atgacgtcaa aaggatccgg ataaaacatg gtgattgcct cgcataacgc ggtatgaaaa
     2581 tggattgaag cccgggccgt ggattctact caactttgtc gacttgagaa agacctggga
     2641 tcctgggtat taaaaagaag atctatttat ttagagatct gttctattgt gatctcttat
     2701 taggatcgca ctgccctgtg gataacaagg atccggcttt taagatcaac aacctggaaa
     2761 ggatcattaa ctgtgaatga tcggtgatcc tggaccgtat aagctgggat cagaatgagg
     2821 ggttatacac aactcaaaaa ctgaacaacg gttgttcttt ggataactac cggttgatcc
     2881 aagcttcctg acagagttat ccacagtaga tcgcacgatc tgtatactta tttgagtaaa
     2941 ttaatccacg atcccagcca ttcttctgcc ggatcttccg gaatgtcgtg atcaagaatg
     3001 ttgatcttca gtgtttcgcc tgtctgtttt gcaccggaat ttttgagttc tgcctcgagt
     3061 ttatcgatag ccccacaaaa ggtgtcatat tcacgactac caataccgat tgcgccaaag
     3121 cggaccgcag aaagatcggg cttctgttcc tgcaatgctt catagaaagg agaaaggttg
     3181 tccggaatat ctccggcacc gtgggttgag ctgataacca gccagatccc agaggcagat
     3241 aaatcttcca acagcggacc gtgcagcgtt tcggtggtaa aacccgcctc ttccagcttt
     3301 tcagccaggt gttctgctac atattcggca ccgccgaggg tgctgccgct gataagagtg
     3361 atatctgcca taaaccgcca cctttattaa gagtggcgta ttgtacgctg tgaacgcgtt
     3421 gggatctacc tgtggaaaag tatgggatta aaaaagccga tcagggcttg atggtacgca
     3481 tgatcgggtt ttgcaggacg atcaatgtct cggtggactg aatttcatca attgtttgga
     3541 tcttgttgat aagtacatgc tggagagcgt cgatcgaacg gcacatcact tttataaaga
     3601 tgctgtagtg gccggtggtg taataggctt cagtgacttc atcaaggctt tccagtttcg
     3661 ccagcgcgga aggatagtct ttggcgctct ttaatataat gccgataaag cagcctacgt
     3721 cataaccgag ctgcttcggg ctgacatcaa tacgcgcccc ggtaatgatc cccgcctgct
     3781 tcattttctc tactcgaacg tgaatcgtcc ccggactgac gccaaattgt ttcgccagtt
     3841 cggcgtaagc ggtgcgcgca ttgcccatta atgcttccag gatgccacgg tccagattgt
     3901 cgattagata attttccata ggattttctt atgcggattg atgattcatt ctattttagc
     3961 cttctttttt aatgaatcaa aagtgagtta ggctttttat tgaatgatta ttgcatgcgt
     4021 gtcggttttt gttgcttaat cataagcaac aggacgcagg agtataaaaa atgaaaaccg
     4081 cttacattgc caaacaacgt caaattagct tcgtgaaatc tcacttttcc cgtcaactgg
     4141 aagaacgtct ggggctgatc gaagtccagg cgccgattct tagccgtgtg ggggatggca
     4201 cgcaggataa cttgtcgggc tgtgaaaaag cggtgcaggt aaaagtgaaa gctctgcctg
     4261 atgcccagtt cgaagtggtt cattcactgg cgaagtggaa acgtcagaca ttagggcaac
     4321 acgacttcag cgcgggcgaa gggctgtaca cgcacatgaa agcccttcgc cccgatgaag
     4381 accgtctttc tccgttgcac tcggtctatg ttgaccagtg ggactgggaa cgcgtaatgg
     4441 gcgacggtga gcgtcaattc tcgactctga aaagcacggt agaggcgatc tgggcgggaa
     4501 ttaaagcaac cgaagctgcg gttagcgaag agtttggcct ggcaccgttc ctgccggatc
     4561 agatccactt cgtacacagc caggagttac tgtctcgtta tccggatctc gatgccaaag
     4621 ggcgtgagcg ggcgattgcg aaagatcttg gcgcggtatt cctcgtcggg attggcggca
     4681 agttgagtga tggtcatcgt cacgacgtgc gcgcaccgga ttatgatgac tggagcaccc
     4741 cgtcagagct gggccatgcg ggtctgaacg gcgatattct ggtatggaac ccggtactgg
     4801 aagatgcgtt tgagctttct tccatgggga tccgcgtgga tgccgatacg ttgaaacatc
     4861 agctggcgct gaccggtgat gaagatcgcc tggagctgga gtggcatcag gcgctgctgc
     4921 gcggtgaaat gccgcagacc atcggcggcg gtatcgggca gtctcgtttg accatgctgc
     4981 tgctgcaact gccacatatc ggccaggttc agtgtggtgt atggccggca gctgttcgcg
     5041 agagcgtccc ttctctgctg taataattta tcgccgccag cgtctgagca ggcggcttcg
     5101 catcccggta tcaaagcgcc agatatgatc gaaaatgcgc atgatgccgg gtttgccatg
     5161 tgcagacatc gccacggcat gaaagcgatg ctgatgtacg cgctgtagct ctttcacttt
     5221 actcgtcacg tcgtcaggca accgctgggc gataaaatca gaaatcacca ccgcatcggc
     5281 atcaaaccat tccctgcttt gcaagcgttc cataatggcg cgaaaacaac tggcaagatc
     5341 ggtgccgcca cgaaactgct ggcttaaaaa acggattgct tgttcgatgc cttgtgggcc
     5401 tgaaagctca taacggacga tctcggtgga aaataacata atatagcagc gccggttttc
     5461 agcgagagca atgcgcatca aggccatgca gaacgctttc gcacactgtt cattaaagcc
     5521 gcccattgag ccggaagtat ccacacagac aataaacggt ccgcgcggct gttcgtcgta
     5581 atctttatgt accaccgggc gttcgatcac tttttcacgc cacgactcac cgtgcaggcg
     5641 ataggtgagc aactgttttt ccaccagccg acggtaaaac tcatactcca gttccgttat
     5701 ccccagtgtc gccagttctg gcggcaggag acgtaaaata tcatcgcttt gttgcagacc
     5761 atcaacctgc tcaggaaccg tcgccggttc gcgcaccatg gtgcggaagg tttccatctg
     5821 cgcatcgttg cgcggtattg atttggcttc ccgtgaacgc cccaactgtt ctgccaggcg
     5881 tttcagttcc ggctgttcgt taagaaattc accgtatttc acaatcaact gatagtcgcc
     5941 gcgtttaagc tgaccggcgc tcatatccca cagacgacca gctgcggtat tgttatctgc
     6001 gagaatcggt tcaagttgcc cgctcagcgt catgcgttcc tgaacttcac tcaacagttg
     6061 ttcgcgttct tcttctaata gctgctgatt tagcgtcgtt gcttgcacga tcagacttaa
     6121 acgccagcgc tggagaaaaa gcgtgtgtaa cgctgaagtg atcgtactgt tagcatcaac
     6181 caactgtcgg gcttgttctg cccatggaga attcagacga tgcagtaagt ccaggatctg
     6241 tggtagctgc acaataaact gcggcgtgga gaggagctgg ctttgctgat agcacatcac
     6301 ctcttcggtg agttccggcg ggactcgggc atctttcagc cgactgcgca gcgcctcacg
     6361 ccagcgggga acatcatcag tgattgccgc cttcagtcgt gggaattttt caaagaagac
     6421 cgccagctgc ggtgaggcca gcagcgcgat gatcatctct tcgatcaatc cctcttcgct
     6481 gacggccagc atcacattaa gcgtatccag cgttagcatt gttgtgcctg gcgaatctgg
     6541 gcgccgacat cctgtaggct ggcttcaatg cgacctaacc agtcgccagg gataaacaga
     6601 catttttgct gttcgctgaa taaagcgtgt tgcttgcgcc agtcgctttc aagctcttcc
     6661 agttgttgtt ttatttcacc tggcagacct tcagccgatg aaccgggaag tgccagcgta
     6721 ctgccttgta aacttacatc gcgtacaaca agatgttggg cgctatccac ttccagattc
     6781 aatttctggg caaagccgat accgttcagt ttgccgcgaa tttcaccacc tttactcagc
     6841 cactgttcca gcgcgttacg ctcaaaggag atatgaacca cttccatatc atgcagtttt
     6901 aacggttttt gcagcagcag agtcagagtg gaagcagtaa cgttaacagg aagttgatac
     6961 tgctgacgac ggctgaaaat gccgcccaga cgaattaccg ttaaggctgt tttatcgctt
     7021 tgttgctgct ggagttgcag gtgacgttgc acaatcgcgc ccaggcgggt caacattcct
     7081 tgctgttgcc aggcatgacc ggtcatcaat acatcaattt gttgttgtat caaattcagg
     7141 ctttgcgcgt cataccacag gcaatctttc agcaaaatga gatcaaccgg ggcaacagca
     7201 ctgcgaccgc taaaaaaggc gctggcctgc aataatcgaa tcgctttttt ccagcgacga
     7261 tccgagacat aaggcgcatc cggtaattta tccagttgct ggcgcagcat aaaaatcagc
     7321 tcaaatacat gatcgggcag cgtaatttca ccaatctctt tctgccagcg ttcatattct
     7381 tcatctgtga cctgcaagga ggcaggaacc ggattgtcgt tttcatcctg ttgactagtc
     7441 agcatggagc ggaaattcgc tttatcctgc actttatcta accacagacg aatcagcatg
     7501 cggtcatata acgcttccag actgctgtct gcttccggca gctcgttgga ggccgccacc
     7561 agcaggcgca tcgggatttt ttctacgtgt gcaccgttgc ggaactggcg ctcgttaatg
     7621 gcggtgagca gggtattaag aattgccgga cccgctttcc agatctcatc cagaaagacg
     7681 atttctgctt ccggcaggta accgctggtt aaacgttcat agcgcccttc atcttttagc
     7741 gcctgaatag aaaggggacc aaaaacctct tccggcgtgg agaagcgagt catcagatat
     7801 tcaaacgcgc gggcattctg aaaggcgaat tttaagcgcc gggcgatcaa acttttggca
     7861 atacctggcg ggccaaggag gaacacactt tcaccactta atgccgctaa taaacacaag
     7921 cggatggcgt ggctacgttc ataaagcccc ttttccaacg aactgctcag gcgggaaatt
     7981 ctttccgcta ataaatgagg gtgagccata atgaagtggc gtcctttcgt caaaagttct
     8041 gcgtaaattg cgagtataga cgtttcttgc tggtggctaa aatagtctca aagggggggt
     8101 atttttcttt gagccaggtt aatgtgcccg catttaggag tacgattttg ccgttaatcg
     8161 tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat atttcaacga
     8221 aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc ctcgcggcga
     8281 ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt gaatgtttgt
     8341 ccggccagtt tggttttggc gttgagcgcg atgccgtgtt tggcttttta tcgctgatct
     8401 tctggctgct aatctttgtg gtttctatta aatatctcac cttcgtgatg cgggcagata
     8461 acgccggtga aggggggatc ctgacgttga tgtcgcttgc cgggcgtaat acgtcggcgc
     8521 gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc tatggtgaag
     8581 tcgtcataac acccgctatt tcggtgatgt ccgccattga aggtctggaa atcgtcgccc
     8641 cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta ttatttatga
     8701 ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg ctgacctggt
     8761 ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa gtgctgcatg
     8821 cgctgaatcc gatgtgggcg gtgcatttct tccttgaata caaaacggtt tcttttattg
     8881 cattaggggc agtggtgctg tcgattacgg gggtcgaggc gctgtatgct gatatggggc
     8941 actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct tccttgaccc
     9001 ttaattactt cggtcaggga gcgttgttgt taaagaaccc ggaagcgatt aagaacccgt
     9061 tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc gcactggcga
     9121 cggtaattgc ctcgcaggct gttatctctg gcgtcttctc attgacgcgt caggcggtac
     9181 gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag tcagggcaaa
     9241 tctatattcc ctttgtgaac tggatgctct atgtcgcggt cgtgattgtg attgtcagct
     9301 ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga accatggtgc
     9361 tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat aagtattttg
     9421 ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc gctaacctcg
     9481 ataaactgct ctccggcggc tggttgccat tgagcctcgg tacggtgatg tttatcgtga
     9541 tgaccacctg gaagagcgaa cgtttccgtt tgctgcggcg gatgcatgaa catggtaact
     9601 ctctggaggc gatgattgct tcgctggaga aatcaccgcc cgttcgcgtg cccgggaccg
     9661 cggtgtatat gtcacgtgca atcaacgtca ttccctttgc gctgatgcat aaccttaaac
     9721 ataacaaggt attgcatgag cgggtgattc tgttaactct gcgcaccgaa gacgcgccat
     9781 atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc tggcgcgtgg
     9841 tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac cgctgcggtc
     9901 tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat gagtcgttga
     9961 tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg ctgctgcaac
    10021 gtaatgcgct gcgtgcgccg gatcaatttg aaatcccgcc aaacagggtt atcgaactgg
    10081 gtactcaggt cgaaatctaa cgccagacgc ctcctttctc cttaaggggg cgtttctgtt
    10141 ttcatggtta attaccatgt aaaacgtttc gaggttgatc acatttccgt aacgtcacga
    10201 tggttttccc aactcagtca ggattaaact gcgggtcagc gaaacgtttc gctgatggag
    10261 aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat ctcccgtctg
    10321 ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa aagtacaacg
    10381 cgtatcgata tggcattaac ccagggcgta ccttctttta tgcaggtgct gggcgtcgtc
    10441 acaaatgaaa tgcaggtcga ggcagccatt atcgcggaag agatcaaaca acataatccg
    10501 catctccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca gggaaatacc
    10561 attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga aagtcaggcg
    10621 gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc tggcgtgacg
    10681 ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag ccttcccggg
    10741 cgtaaaagcc ctctcgggcg cagcgttaaa tgtctatcct ggccgcgtga tggcgctggt
    10801 gggcgaaaac ggtgcgggta aatccaccat gatgaaagtg cttacgggta tctatactcg
    10861 cgatgccggt acgcttttat ggctggggaa agaaacgacg tttaccgggc cgaaatcttc
    10921 ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc agttgaccat
    10981 tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa ttgactggaa
    11041 aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
    11101 caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaattg ccaaagtgct
    11161 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
    11221 aaccgaatcc ttgttccgcg tcatccgcga gctgaaatcg caaggccgcg gtattgtcta
    11281 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
    11341 tgggcaattt attgctgagc gtgaagtagc atcactgacc gaagattcgc tgattgagat
    11401 gatggtgggt cgcaagctgg aagatcaata tccgcacctg gacaaagcgc cgggagatat
    11461 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
    11521 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
    11581 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
    11641 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
    11701 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
    11761 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggcagtaag
    11821 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
    11881 ctccggtggc aatcagcaaa aagtggcgat tgcccgtggt ctgatgacac gccccaaagt
    11941 gttgatcctc gatgagccta cccgtggcgt agatgtcggc gcgaaaaaag agatctatca
    12001 actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat cggagatgcc
    12061 agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc tcagcgggga
    12121 atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg gcaagcttaa
    12181 tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc cgttatttca
    12241 cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg atcgcgattg
    12301 tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt ctccagcaaa
    12361 cctcggtgaa cgccattatg gcagtcggga tgacgctggt gatcctgacg tcgggcatcg
    12421 acttgtcggt aggttctcta ttggcgctga ccggcgcagt tgctgcatct atcgttggca
    12481 ttgaagtcaa tgcgctggtg gctgtcgctg ccgctctcgc gttaggtgcc gcaattggtg
    12541 cggtaaccgg ggtgattgta gcgaaaggtc gcgtccaggc ctttatcgct acgctggtta
    12601 tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca gtgaatactg
    12661 gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg ctgggtgtac
    12721 cgacgccagt ctggatcatg ggaattgtct tcctcgcggc ctggtacatg ctgcatcaca
    12781 cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg cgcctttctg
    12841 gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg ctggcatcgc
    12901 tggccgggat cattgaagtg gcgcgtctct cctccgcaca acctacggcg gggactggct
    12961 atgagctgga tgccattgct gcggtggttc tgggcggtac gagtctggcg ggcggaaaag
    13021 gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat aatggattga
    13081 atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg attttgctgg
    13141 cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg aatatgaaca
    13201 tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc agtgcgaatg
    13261 cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg ttctttgtat
    13321 cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg ctataacctg gtggtgctgg
    13381 actcccagaa taacccggcg aaagagctgg cgaacgtgca ggacttaacc gttcgcggca
    13441 ccaaaattct gctgattaac ccgaccgact ccgacgcagt gggtaatgct gtgaagatgg
    13501 ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa ggtgaagtgg
    13561 tgagccacat tgcttctgat aacgtactgg gcggcaaaat cgccggtgat tacatcgcga
    13621 agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgccggt acatccgcag
    13681 cccgtgaacg tggcgaaggc ttccagcagg ctgttgctgc tcacaagttt aatgttcttg
    13741 ccagccagcc agcagatttt gatcgcacta aaggtttgaa cgtaatgcag aacctgttga
    13801 ccgcacatcc ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc
    13861 tgcgcgcact gcaaactgcc ggtaaatcgg atgtgatggt cgtcggattt gacggtacac
    13921 cggatggcga aaaagcggtg aatgatggca aactagcagc gactatcgct cagctacccg
    13981 atcagattgg cgcgaaaggc gtcgaaaccg ccgataaagt actgaaaggt gagaaagttc
    14041 aggctaagta tccggttgat ctgaaactgg ttgttaagca gtagttttaa tcaggttgta
    14101 tgacctgatg gtgacataaa tacggcatcg acagatgaac gtgtaatata aagaaaagca
    14161 gggcacgcgc caccctaaca cggtggcgca ttttatggac atcccgaata tgcaaaacgc
    14221 aggcagcctc gttgttcttg gcagcattaa tgctgaccac attcttaatc ttcaatcttt
    14281 tcctacccca ggcgaaaccg taaccggtaa ccactatcag gttgcatttg gcggcaaagg
    14341 cgctaatcag gctgtggctg ctgggcgtag cggtgcgaat atcgcgttta ttgcctgcac
    14401 tggcgatgac agcattggtg agagcgttcg ccagcagctc gccactgata acatcgatat
    14461 ttctccggtc agcgtgatca aaggcgaatc aacaggtgtg gcgctgattt ttgttaatgg
    14521 cgaaggtgag aatgtcatcg gtattcatgc cggcgctaat gctgcccttt ccccggcact
    14581 ggtggaagcg caacgtgagc gtattgccaa cgcgtcagca ttattaatgc agctggaatc
    14641 accactcgaa agtgtgatgg cagcggcgaa aatcgcccat caaaataaga ctatcgttgc
    14701 gcttaacccg gctccggctc gcgaacttcc tgacgaactg ctggcactgg tggacattat
    14761 tacgccaaac gaaacggaag cagaaaagct caccggtatt cgtgttgaaa atgatgaaga
    14821 tgcagcgaag gcggcgcagg tactgcatga aaaaggtatc cgtactgtac tgattacttt
    14881 aggaagtcgt ggtgtatggg ctagcgtgaa tggtgaaggt cagcgcgttc ctggattccg
    14941 ggtgcaggct gtcgatacca ttgctgccgg agataccttt aacggcgcgt taatcacggc
    15001 gttgctggaa gaaaaaccat tgccagaggc gattcggttt gctcatgctg ccgctgcgat
    15061 tgccgtaaca cgtaaaggcg cacaaccttc cgtaccgtgg cgtgaagaga tcgacgcatt
    15121 tttagacagg cagaggtgac gcttggctac aatgaaagat gttgcccgcc tggcgggcgt
    15181 ttctacctca acagtttctc acgttatcaa taaagatcgc ttcgtcagtg aagcgattac
    15241 cgccaaagtt gaagcggcga ttaaagaact caattacgcg ccatcagccc tggcgcgtag
    15301 cctcaaactc aatcaaacac ataccattgg catgttgatc actgccagta ccaatccttt
    15361 ctattctgaa ctggtgcgtg gcgttgaacg cagttgcttc gaacgcggtt atagtcttgt
    15421 cctttgcaat accgaaggcg atgaacagcg gatgaatcgc aatctggaaa cgctgatgca
    15481 aaaacgcgtt gatggcttgc tgttactgtg caccgaaacg catcaacctt cgcgtgaaat
    15541 catgcaacgt tatccgacag tgcctactgt gatgatggac tgggctccgt tcgatggcga
    15601 cagcgatctt attcaggata actcgttgct gggcggagac ttagcaacgc aatatctgat
    15661 cgataaaggt catacccgta tcgcctgtat taccggcccg ctggataaaa ctccggcacg
    15721 cctgcggttg gaaggttatc gggcggcgat gaaacgtgcg ggtctcaaca ttcctgatgg
    15781 ctatgaagtc actggtgatt ttgaatttaa cggcgggttt gacgctatgc gccaactgtt
    15841 attacatccg ctgcgtcctc aggccgtctt taccggaaat gacgctatgg ctgttggcgt
    15901 ttaccaggcg ctgtatcagg cagagttaca agttccgcag gatattgcgg tgattggcta
    15961 tgacgatatc gaactggcaa gctttatgac gccaccatta accactatcc accaaccgaa
    16021 agatgaacta ggggagctgg cgattgatgt actcatccat cggataaccc aaccgaccct
    16081 tcagcaacaa cgattacaac ttactccgat tctgatggaa cgcggttcgg cttagattta
    16141 cgctgtcttt tgatcaaatt attaccatcg gttgttttca gaagcatgaa cattgctgct
    16201 gaagcaacag taataatgcc catcgtgata aacgtatagt ggaattgttc gacagtcgtt
    16261 gtgccttcca tcccttcata aacgcgaagg acggccgcac ttacagcaac gcctaaactg
    16321 atagacagtt gctgcgtgac cgccagaaca ctgttaccgc tgctggcgtt gtcatcggtc
    16381 agatcggcaa gtgtgatggt attcatcgcg gtaaattgcg tcgacatagc catccctaat
    16441 ataaacaacg gcaagatcag catccatatc gccattgccg gtgattgcaa agagaactga
    16501 gcgatcatta gcccaataat caccgtgatc cccactaatg tatggcgata gcccagacga
    16561 cgtaagactt gggtaaccat cgattttgca ataatggaac ctaacgctgt cggtgccatc
    16621 atacagccgg caataaacgc ctgataacca aatcctacct gtaacatcaa tggcataagg
    16681 aacggtacac aaccggtccc cagacgggtt gcaatattgc ctacgatacc gatcgagaaa
    16741 gtgcgggttt taaataaatc taatgaaatt aatgggtttg gcgtgcgtcg tgcatggaga
    16801 atatagagaa gcagtaaccc gatgctggtg acaattaccg tcaaggcaat ccaactggcg
    16861 acaatctttt ccccgaatag ctctattccg cttgagaaga gaacaaggct aaggccaaac
    16921 agcaaaaagc cagtgatatc gaatctgcgt cgtgcggtgg tgaaattggg catatgtttg
    16981 cgcgcgtaaa gaaggcccgc aatacctatg gggatattga ttaaaaatat ccagtgccag
    17041 gttgcccagg tgaccagcac gccgccaaga acagggccta aaattggccc caccagaccc
    17101 ggcatggcga caaaattcaa tacaggaaga agctcattac gaggataagc gcgcagtaag
    17161 gccagccgag caacaggcat catcattgcg ccgcctatcc cctgaataac ccggaagaca
    17221 accagctgtg gtagcgagtt agaaagtgcg caggccagag aacccaacgt gaacagactc
    17281 acggcaagag taaatatgcg acgcgtaccg aagcgatcgg ctagccatcc gcttaccgga
    17341 ataagcatcg ccaccgtcag cgtataactg atgatggctg attgcatcgc gagaggagaa
    17401 cgattaaggc tatgagcgat tgcgggtaag gcggtattca gaatagtggc atcaagtgcc
    17461 tgcatgaaga aggccatcgc cgcgatccac ggcaaacccg ccatactgcg cttcttttta
    17521 tcgctcattc aatgtcctgt tatcggggtg tcacttatca ggtgagcgta gcagcgcctg
    17581 acaagcttta aatgccgcgt cgccatcgct ttggataatc gcgtcaacaa tcgcctggtg
    17641 cagatccagc tttatcactg tgtcgctggt aattgacgtg aagtaagtgt gataaaccga
    17701 atggaagagc gaggcgaatg aggtcaaaaa cggattggcg ctcatttcat agatatgctc
    17761 atgccaggcc atatcgacct caatccagcg ttcacggcga aagttctctt ttaatgccac
    17821 catttcggtc attaacgtat tgagatgcgc cttctgttcc gcggtgccaa ccattgctgc
    17881 cagtaggcaa gcttgcggct ccagacagat acgcataacc agaaagtgat cgatgacctg
    17941 atgaaagttc tcttctgtca tccaccaggt aagcaattcc tgatcaagaa aattccagtt
    18001 tgattgtggc atgacccggg taccaattcg cggtcgcggt aaaaccatcc cttttgccgt
    18061 taacgttttg accgcttcgc gtaccgctgt acgactcact ccaaattgct cgcccagctc
    18121 aatctcacca ggcaaaatgg tgccgggttc atattcacct tttaagatgc gttgcgccag
    18181 cttctcagcc agaacatacg aaaggttttt ctgtgcagct aactgttgtg cgcttaaagg
    18241 cattactaat cttccttttt ctttttattc ctccttagta tgccaccagg aagtgtgatt
    18301 acggttgcaa aaacggcaaa ttgcttgttt tatggcacat taacggggct tttgctgaaa
    18361 aaatgcgcgg tcagaaaatt att
//
LOCUS       DAZKGF010000096        18070 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZKGF010000096 DAZKGF010000000
VERSION     DAZKGF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.096"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(39..1067)
                     /gene="epmB"
                     /locus_tag="ACHOQV_003106"
     CDS             complement(39..1067)
                     /gene="epmB"
                     /locus_tag="ACHOQV_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP9631441.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1109..1675
                     /gene="efp"
                     /locus_tag="ACHOQV_003107"
     CDS             1109..1675
                     /gene="efp"
                     /locus_tag="ACHOQV_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9631442.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1727..1852
                     /gene="ecnA"
                     /locus_tag="ACHOQV_003108"
     CDS             1727..1852
                     /gene="ecnA"
                     /locus_tag="ACHOQV_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP9631443.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            1963..2109
                     /gene="ecnB"
                     /locus_tag="ACHOQV_003109"
     CDS             1963..2109
                     /gene="ecnB"
                     /locus_tag="ACHOQV_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP9631444.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2284..2601
                     /gene="sugE"
                     /locus_tag="ACHOQV_003110"
     CDS             2284..2601
                     /gene="sugE"
                     /locus_tag="ACHOQV_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP9631445.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2602..3135)
                     /gene="blc"
                     /locus_tag="ACHOQV_003111"
     CDS             complement(2602..3135)
                     /gene="blc"
                     /locus_tag="ACHOQV_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP9631446.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3224..4357)
                     /locus_tag="ACHOQV_003112"
     CDS             complement(3224..4357)
                     /locus_tag="ACHOQV_003112"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP9631447.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            complement(4420..4779)
                     /gene="frdD"
                     /locus_tag="ACHOQV_003113"
     CDS             complement(4420..4779)
                     /gene="frdD"
                     /locus_tag="ACHOQV_003113"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP9631448.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4790..5185)
                     /gene="frdC"
                     /locus_tag="ACHOQV_003114"
     CDS             complement(4790..5185)
                     /gene="frdC"
                     /locus_tag="ACHOQV_003114"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP9631449.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5196..5930)
                     /gene="frdB"
                     /locus_tag="ACHOQV_003115"
     CDS             complement(5196..5930)
                     /gene="frdB"
                     /locus_tag="ACHOQV_003115"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP9631450.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(5923..7731)
                     /gene="frdA"
                     /locus_tag="ACHOQV_003116"
     CDS             complement(5923..7731)
                     /gene="frdA"
                     /locus_tag="ACHOQV_003116"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP9631451.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAVIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8056..9033
                     /gene="epmA"
                     /locus_tag="ACHOQV_003117"
     CDS             8056..9033
                     /gene="epmA"
                     /locus_tag="ACHOQV_003117"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP9631452.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9252..10754
                     /gene="yjeM"
                     /locus_tag="ACHOQV_003118"
     CDS             9252..10754
                     /gene="yjeM"
                     /locus_tag="ACHOQV_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP9631453.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            10817..11131
                     /gene="yjeN"
                     /locus_tag="ACHOQV_003119"
     CDS             10817..11131
                     /gene="yjeN"
                     /locus_tag="ACHOQV_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFP9631454.1"
                     /translation="MDDASRDPVITEDEIRELQFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            11128..11442
                     /locus_tag="ACHOQV_003120"
     CDS             11128..11442
                     /locus_tag="ACHOQV_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFP9631455.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(11473..14796)
                     /gene="mscM"
                     /locus_tag="ACHOQV_003121"
     CDS             complement(11473..14796)
                     /gene="mscM"
                     /locus_tag="ACHOQV_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP9631456.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLLGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14818..15786)
                     /gene="asd"
                     /locus_tag="ACHOQV_003122"
     CDS             complement(14818..15786)
                     /gene="asd"
                     /locus_tag="ACHOQV_003122"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP9631457.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDANPLVDDNKDQV"
     gene            complement(15883..16935)
                     /gene="rsgA"
                     /locus_tag="ACHOQV_003123"
     CDS             complement(15883..16935)
                     /gene="rsgA"
                     /locus_tag="ACHOQV_003123"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFP9631458.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            17030..17575
                     /gene="orn"
                     /locus_tag="ACHOQV_003124"
     CDS             17030..17575
                     /gene="orn"
                     /locus_tag="ACHOQV_003124"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFP9631459.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17786..17861
                     /locus_tag="ACHOQV_003125"
     tRNA            17786..17861
                     /locus_tag="ACHOQV_003125"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17819..17821,aa:Gly,seq:gcc)
BASE COUNT         4161 a         4638 c         4772 g         4499 t
ORIGIN      
        1 atgcaacgtg caaagattaa acttccctgt ttaatcagtt actgctggcg tagctggaga
       61 tccagcggcg ttttgctggg ttcgccgcca atttctcgcg ccagtttagg caccagatat
      121 cccgacacca gtgtcagcaa ctcacgcata atctgccgtg cttcgtcatc actcaccata
      181 aaatgcgccg cgccctgtac tttatcgagc acatgcaggt aatacggcat cacgcccgca
      241 tcgaacaacg cattactcag gtttgccagc gtttgcgcgt tatcgttcac accacgtaac
      301 agaacgctct ggttgagcaa agtaacgcct acccggcgca acttagccat cgcctgacgg
      361 aatgtttcat ctacctcatt ggcatggtta atgtgattca ccagcaagat ttgcagcgta
      421 gaatgagaaa agcgttcaac cagcgcatct gtgatgcgtg ccgggatcac aatcggcaga
      481 cggctgtgaa tccgcagacg tttgatatgc gggatggctt ccagttgtgt gagcaaccag
      541 tccagctcgt gatctttcgc catcagcgga tcgccgccgg agaaaatcat ctcgtccagt
      601 tccggatgcg cagcaacata ctcaagtgca gtctgccagt tacgcttgtt gccctgattt
      661 tcggcatagg ggaagtggcg acggaagcaa tagcggcaat ttaccgcgca gccgcctttg
      721 accagcaaaa gcgcccggtt gtggtattta tgcaacaaac ctggcactac gctgtgctgt
      781 tcttccagcg ggtcggtgga gaatccgggc gcgacaacaa actcatcttg cgaggtaagt
      841 acctgacgca aaagcggatc gttcggattg cctttctcca tgcgatcgat aaatgagcgg
      901 ggcacacgca gggcaaacag ctttttggcg ctgcgcccgg ccaacagttt ttcgtccgcg
      961 tctatattca aaagacgcag aagttcatca ggatcggtca caacatcggc aagttgcgtt
     1021 aaccaatctt ctctggatgg ggtatttagg gttacaatat gcgccatttt gtggcttagc
     1081 taccaattaa caaatttcag agggccttat ggcaacgtac tatagcaacg attttcgtgc
     1141 tggtcttaaa atcatgttag acggcgaacc ttacgcggtt gaagcgagtg aattcgtaaa
     1201 accgggtaaa ggccaggcat ttgctcgcgt taaactgcgt cgtctgctga ccggtactcg
     1261 cgtagaaaaa accttcaaat ctactgattc cgctgaaggt gctgatgttg tcgatatgaa
     1321 cctgacttac ctgtacaacg acggtgagtt ctggcacttc atgaacaacg aaactttcga
     1381 gcagctgtct gctgatgcaa aagcaattgg cgacaacgcc aaatggttgc tggatcaggc
     1441 agagtgtatc gtaactctgt ggaatggtca gccgatctcc gttactccgc cgaacttcgt
     1501 tgagctggaa atcgttgata ccgatccggg cctgaaaggt gataccgcag gtactggcgg
     1561 caaaccagct accctgtcta ctggcgctgt ggttaaagtt ccgctgttcg tacagatcgg
     1621 cgaagtcatc aaagtggata cccgctctgg tgaatacgtc tctcgcgtga agtaatgcgg
     1681 ttgtggtgcg gcctgcaggc tgcaccatca cttattcagg tcagagatga tgaaacgcct
     1741 tatcgttctt gttttgcttg ccagtgcgct gctcacgggc tgtaacaccg ctcgcggttt
     1801 tggtgaagac attaaacatc tcggcaactc catctcccgc gctgccagct aatttttctt
     1861 ctcctccgaa aaatcatcag atttccatca tttttggcga tgttgtctat tattaatttg
     1921 ctataggcaa acataaataa cattacctaa aaggaagacg ttatggtgaa gaagacaatt
     1981 gcagcgatct tttctgttct ggtgctttca acagtattaa ctgcttgcaa caccacgcgt
     2041 ggcgttggtg aagacatttc tgatggcggt aacgcgattt ctggcgcagc aacgaaagcg
     2101 cagcagtaag caataacggt acgacagctg tgtcgtgccg tttgtttttt tgcgatagtc
     2161 acaaaggtaa tagttgaaat tcccctgcca cctggcaaaa tatccgttca accatcagct
     2221 ttgcaggacg acctgcaaac gcctcttttc accggggacg gccccaattc tccggagcct
     2281 gatatgtcct ggattatctt agttattgct ggcctgctgg aagtggtatg ggccgttggc
     2341 ctgaaatata cccacggctt tagtcgtttg acgccaagtg ttattactgt gacggcgatg
     2401 attgtcagta tggcgctact tgcctgggcg atgaaatcgt taccagtagg gacggcttat
     2461 gccgtgtgga cgggtattgg cgcagtcggc gcggccatca ccggcattgt gctgctcggc
     2521 gagtccgcta acccgatgcg cctggcgagt ctggcgttaa tcgtattggg gattattggt
     2581 ctgaaactca gcactcacta actaactacc gggctgctgt acccaaataa acttactgac
     2641 atcaaaccct tcccgggtcg cgactgccag catctcctgt ttcacttcgt cagaaatggt
     2701 tggcgtgcgg gagagtatcc acaggtagtc gcggtccgga ccgcaaacca gcgcatggcg
     2761 gtattcccga tcgagtgcaa taacgttata accgccatag aaaggaccaa agaatgacac
     2821 tttcagcgca gcgcgagttg gtgcgccggt aaagtacgct ttcccttcac tctgctgcca
     2881 catttctctg tcagggttat agcctttatt aatgacattc aggccgccgt catcacgcag
     2941 gctgtatgtt gcggtgactt tttccagtcc acgttcaaaa cggtgatcaa aacgggcaat
     3001 ctcataccag gtaccgagat agcgtttagc atcgaaatta ttcactacgg tcacgccacg
     3061 gggcggcgta ggagaactgc aggcaacgac cagaaatgca gctgtcgctg cggcaacgag
     3121 agggagcagg cgcataaatg tttccttact ggtttttttc taagtgtaga tgacagcaag
     3181 gaaaagcgga gaaaaggtcc aaaaattcgg acccgatgaa attttactgt agagcgttga
     3241 gaatctgaca ggcggcggcg actctcgctg gattgggata gtttttgtta gccagcatca
     3301 cgatacccag ctctttttct ggaataaacg cgacatagct accaaatccg ccggtcgccc
     3361 ccgttttatg tacccatgat gcgcgtactg caggagttgg gggcgtaatc gcttttacgg
     3421 ggcgtgctgc cagtgcaatt ttattgtcac tgccgttaat gatgatgtca ggattgaccg
     3481 gccagtccag catttcccag cccaggccct gatacatatc gccggtttgc cagtagcgag
     3541 attgtgccag ttgtatccct tgttgaagcg ttttctcagt gatatcaagg ggttttaaat
     3601 tgctttgcac ccagcgggcc atatcttcaa tggtcgactt cacaccataa gcctcagcat
     3661 ctaacgcccc tggcgaaaca tgcactgcct taccttcgcg atatccccag gcgtaattct
     3721 tttcttctgc ggacggcaca ttaatccacg tatgagtgag tttgagtggc tggaaaacac
     3781 gagtttgcat cgcctgttca aagctcaaac cggatggttt caccgccagt gcaccgaaca
     3841 acccaatact ggcgttggca tacagacgtt gtgttcctgg agcccatgca ggttgccagt
     3901 tttgatagaa gcgcagcaag tcgcttgagg atttaacgtc atccggcacc tgcaatggca
     3961 ggccgccagc ggtgtaggtc gcgagatgta atagtgtgat cccattccat tgtttagcgg
     4021 taagttcagg ccagtatttt gtcgcgggat cgcttaactt gatttcccct cgagcaatag
     4081 cgtcgccacc aagcacgccc gtaaatgttt tgctgaccga acctaactca aacaacgttt
     4141 gctgtgtgac gggctgcttt ttggcgatgt ccgcatagcc ccaggtaaag taataaggtt
     4201 taccctgata aattaccgcc accgctatac cggggatctt ttgttgctct ataagcgggg
     4261 taattgtgcg atgcacaata tcgttgattt gttgaggggc agcaaatgtg gagcaagagg
     4321 cggtaattaa taaggcgcag agcgtcattt tgaacatagg gtctggtttc catacaaaac
     4381 ggcccgccat aggcgggccg gatttacatt ggcgatgcgt tagattgtaa cgacaccaat
     4441 cagcgtgaca actgtcagga tagcagccag accgtagaaa acccatttgc ccgcaggtac
     4501 gtggattttc agatcgtgca tcgcgtggtg catacggtgt aaaccacacc acagcggcag
     4561 aacgatcatc aggaacagga atacgcgacc aatgaagctc tgcgcgaacg ccagaacgcg
     4621 ctcgtagctc agtgcgtcac ccggaaacag ccccagtggc agcagaatac caaccagcag
     4681 gatcatcacc ggcgcaataa ttgcgctcca cataccaccg gccccgaaga ggccccagaa
     4741 taccggttcg tcagaacgct ttggatttgg attaatcatc tcaggctcct taccagtaca
     4801 gggcaacaaa caggattacg atggtggcaa ccacagttac cgcccagagg cttttgataa
     4861 ttggctctgg tcccattttt tcgtctttta caatgatatt ggccgctttt ggtgccagct
     4921 caaaccaggt tttggtgtgc agcagggctg ccgccagagt gatcaggtta atgatcacga
     4981 taaccgggtt ttgtaaaaag tcgacgaatc ccgcccaggc ttccgggcca tttttcaggg
     5041 caaacagccc gaaaatcagt tcaatgctga accacacagc cggaaccgcc gtgccttcgc
     5101 gcagcatgta gaagcgataa aacggcaatt ttttccacca ggtggacgtc attggccgta
     5161 catacggttt acgtttagtc gtcatgttgc actccttagc gtggtttcag ggtcgcgata
     5221 agaaagtctt tcgaactttc tactttgccc tgctgaatgg ccgcagccgg atcgacgtgt
     5281 ttcgggcaga cttcggagca gtagcccacg aaagtacagc tccatacgcc gttctggctg
     5341 ttcaactgcg ccatacgctc cttcttaccg tggtcgcggc tatcttcgtt ataacgatgc
     5401 gccagcgtaa tggcagccgg accgatgaac tctgggttca ggccaaactg cgggcacgcg
     5461 gcgtagcaca gaccacagtt gatgcaaccg gagaactggt gatacttggc catctgcgcc
     5521 ggggtctgga tgttagtacc ctgatccgcg gtgcgggagt tgccgatgat gtacggtttg
     5581 atcgcttcca gactttcgat gaagtgggtc atatcgacca ccagatcgcg ttcaatcggg
     5641 aagttagcta acgcttcaac cttcataccg tcggtgtaat cacgcaggaa ggttttacat
     5701 gccagttttg gcacgttgtt aaccatcatg ccgcaggaac cacaaatcgc catacggcag
     5761 gaccagcggt agctcaggtc cggtgccagg ttgtctttga tgtagcccag cgcatccagt
     5821 aatgaggtag ttgcgtcata aggcacttca tagaatgcgc tatgcggtgc ggtatcgact
     5881 tccgggttat agcgcaccac ctcaattttc aggtttttca tctcagccat tcgccttctc
     5941 cttcttattg gctgcttccg ccttatcggc tgcatccgct tcgccaccgt aaacgcgttt
     6001 agctggcggc agcgtagtaa tcttcacgtc gctgtactcc aggcgagtcg tgccatcagc
     6061 atcgcggaag gcgagggtgt gtttgaggaa gttgacgtcg tcacgctcgg tgcaaccttc
     6121 gtccagacgc tggtgtgcgc cgcgggactc tttacgtgcc attgcggagt gcgccataca
     6181 ttcagcaacg ttcagaccgt ggcccagttc aatggtgtag agcaggtcgg tgttgaacac
     6241 gctggaagtg tcggtgatgc gcacgcgttt gaagcgttcc tgcagctctg ccagcttgtc
     6301 gatggttttc tgcatcagtt ccggcgtacg gtagataccg cagccttctt ccatagccag
     6361 gcccatttcg tcgcggatct tcgcccagtt ttcgccgcca tcctggttaa ccagatcttt
     6421 caaacgttgt tcaacgccag ctgcctgcgc ttcaattacc gcttcgttgc cattaccggc
     6481 agttgctgca cgctctgtcg cttgttcacc ggccagacgg ccgaagacca ccagttccgc
     6541 cagggagtta gaacccagac ggtttgcacc gtgcagacca acagaggaac attcacccac
     6601 ggcgaacaga cctttaatgc gggtttcaca gttctgatcg gtttcgatac cgcccatggt
     6661 gtagtgtgcg gtcggacgta ccggaatcgg ttctttaacc ggatcgacgc caacgtacgc
     6721 tttcgccagt tcgcagatga acggcagacg ttcatgcagt tttttctcgc cgaggtgacg
     6781 caagtcgaga taaaccacat cgccacgcgg cgtggagata gtgttgcctt tacgccattc
     6841 gtgccagaag gcctgagaaa ctttgtcgcg tggacccagt tccatatatt tgtttttcgg
     6901 ctcgcccagc ggagtttccg ggcccatgcc gtaatcttgc agataacggt agccattttt
     6961 gttgaccaga ataccgcctt caccgcggca gccttcggtc atcaggatac cggaacctgg
     7021 cagaccggtt gggtgatact gaacgaattc catgtcacgc agcggaacgc cgtggcttag
     7081 cgccataccc ataccgtcac cggtaacgat gccgccattg gtattgtaac gataaacgcg
     7141 acccgcaccg ccggtagcca taacgaccgc gttagcacgg atctgcacca gcgtgccttc
     7201 catcatgttc attgctacca ggccgcgaac atgaccatca tcaaccagaa tatccagtac
     7261 gaaatgttcg tcaaaacgct ggatctgcgg gaattgcaga gaggtctgga acagcgtgtg
     7321 cagcatatgg aagccggtct tatcggcggc gaaccaggtg cgctcgattt tcatgccgcc
     7381 gaagcgacgt acgttgacgc taccatccgg gcgacggctc catgggcatc cccacagttc
     7441 cagttgggtc atttcggtgg ggcagtggtg gacgaaataa tccacgacat cctgctcaca
     7501 caaccagtcg ccacccgcta ctgtatcgtg aaagtgatat tcgaagctgt catgatcctg
     7561 cgcgacagcg gcggagcccc cttctgcagc aacggtatgg ctacgcatcg ggtatacttt
     7621 tgagattagt gcgatttttg catttggatt tgcctgcgcg gcagcaattg cagcacgtaa
     7681 tcccgcgcca ccggcgccta caatggcaag atcggcttga aaggtttgca cgacattcct
     7741 ccagattgtt tttatcccac agccccgtac ttcagggtaa gtgcctgaaa gttacggtct
     7801 gcgaacgcta ttccactgct cctttatagg tacaacagta tagtctgatg gataagtctg
     7861 aaatttgacg agatcgattt ttttagtgcg caagagggct aaattatcac tgaagatgat
     7921 taatttaatt actaaaccat cagatcgtgc tttttttagt cactgcccac ccttgtttga
     7981 ttcctgcgta aaatttgtct cgccgtcgcg ttgcgagtag acttcgtgcc cttgtcaaaa
     8041 actggagatt taactatgag cgaaacggca tcctggcagc cgagcgcatc cattcctaac
     8101 ttattaaaac gcgcggcgat tatggcggag atccgtcgtt tctttgccga tcgtggagtg
     8161 ctggaggtgg agacgccttg tatgagccag gcgacggtaa ccgatattca tttggtcccg
     8221 tttgagacac gtttcgtagg ccccgggcat tcgcagggga tgaatctctg gttaatgacc
     8281 agcccggaat accatatgaa acgcctgctg gttgctggtt gtgggccggt attccagctg
     8341 tgccgcagct tccgtaatga agagatgggg cgttatcaca accctgagtt cactatgctt
     8401 gagtggtatc gaccgcacta tgatatgtac cggttgatga acgaggtgga cgatctcttg
     8461 caacaggtgc tggactgtcc ggcagcagaa agcctttctt atcaacaagc tttcttgcgt
     8521 tatctggaaa ttgacccact ctctgccgac aaaacgcaac tgcgggaagt ggcagcgaaa
     8581 ctggatttga gcaacgttgc cgatactgaa gaagaccgcg acacgcttct acaattgctg
     8641 tttacctttg gcgtagagcc aaatattggc aaagaaaaac cgacctttgt gtaccacttt
     8701 ccagccagcc aggcatcact ggcgcaaatc agtaccgaag atcatcgggt cgctgaacgc
     8761 tttgaggttt attataaagg tattgagctg gcgaatggtt tccatgaatt gacggatgcc
     8821 cgtgagcagc aacaacgctt tgaacaagat aaccgtaagc gcgcggcgcg cggtttgccg
     8881 cagcacccca ttgaccagaa tctgattgaa gccttgaaag tcggtatgcc tgactgttcc
     8941 ggcgtggcat taggcgttga tcgtctggtg atgttggcgc tgggcgcgga gacactggct
     9001 gaagtcatcg cctttagcgt tgaccgggca taattctgaa aaattactga attaaccgcg
     9061 aagaatggcg tgataagcgc cattctgtag catatgtttt catttttccg ctaaaggcca
     9121 caatttaaga ctggatattt gatatcatcc aggtatcaat tctgtattgg tttttacgct
     9181 gcccgctcaa tgttgggcag tgcagttcag tgggcgcgtt ttatacacgc gctgaaatga
     9241 aggatgtttt catgcctcac acgataaaaa agatgagtct gataggactc atattgatga
     9301 tctttacttc cgtatttgga tttgccaata gcccatcggc ttattactta atgggttata
     9361 gtgcgattcc cttttatata ttttctgcat tgttattctt tattccattc gccttaatga
     9421 tggctgaaat gggagctgct tatcgcaaag aagaaggcgg tatctattcc tggatgaata
     9481 atagtgtcgg accacgtttt gccttcattg gtacgtttat gtggttttcc tcttatatca
     9541 tctggatggt gagtacctcc gcgaaagttt gggtaccgtt ctcaacattc ctctatggta
     9601 gcgacatgac ccagcactgg cgtattgccg gactggagcc tacgcaggtg gttggtctgc
     9661 tggcagtggc atggatgatt ctggtcaccg tcgttgcttc aaaagggatt aataaaattg
     9721 cccgcattac tgcggtgggc ggtattgcag taatgtgtct gaatttagta ttgctgttag
     9781 taagcattac tattttgtta ttaaatggtg ggcatttcgc gcaggatatt aatttccttg
     9841 catcaccgaa cccaggttat cagtccggtc tggcaatgct atcgtttgtg gtatttgcta
     9901 tttttgccta tggtggaatt gaagcggttg gtggtctggt cgataaaacg gaaaatccag
     9961 aaaagaactt tgccaaaggt attgtttttg ccgctattgt tatttcaatc ggttattcgc
    10021 tggcaatatt tttatggggc gtcagcacaa actggcagca ggtattaagt aatggttccg
    10081 ttaacctcgg caatattacc tatgtgctga tgaagagcct cggggtgacg ctgggtaacg
    10141 cactgcattt gtcacctgaa gcgtcattgt cgctgggcgt atggtttgcg cgtattaccg
    10201 gactttcgat gttcctcgct tataccggtg cgttctttac gctttgctat tcaccgctga
    10261 aagccatcat ccaggggacg ccgaaagcgt tgtggccgga accgatgacg cgcctgaatg
    10321 cgatggggat gccgtctatc gccatgtgga tgcagtgcgg gttggttact gtcttcatcc
    10381 tgctggtttc gtttggtggc ggtaccgcat cggcgttctt taacaagctg acgctgatgg
    10441 cgaacgtgtc tatgacgctt ccttacctgt tcctcgcgct ggctttccca ttctttaaag
    10501 cacgtcagga tctcgacaga ccgtttgtga ttttcaaaac gcatatgtcg gcaatgattg
    10561 caacagtggt tgtcgtactg gtggtgacat ttgcgaacgt cttcaccatc attcaacctg
    10621 tggttgaagc tggagactgg gacagcacat tgtggatgat tggcggccct gtcttcttct
    10681 cgctgttagc gatggcgatt taccagaact attgcagtcg catggcgaac aaacctgagt
    10741 tagctctcga ctgatcatat gaaatcctgc aactcatcgt ctctccggcg atgagttgtc
    10801 tggaaaggaa gacgttatgg acgacgctag tcgggatcca gtgataaccg aagatgaaat
    10861 cagggagctg caattttctg ccggggatgt ggcggaaata gagcaaacgg tcctctcttt
    10921 tgtcgatacc cgccacaccc gcaaagtagc gatggtggtg ggtaacacta ttaataccct
    10981 caaagagcgc gatggtccgc ggtggggaaa cctgccggat atctattgcg cttatcttat
    11041 tagatgtctg gtttttcgcg gagaattagt gggatacggc gatttgtttc gtatgcgcta
    11101 tagcgaaatt aagcgaccga ttatctcatg agtgcgcgca tgtttatctt gtgctgcatc
    11161 tggtttattg tggcgttcct ctggataacc atcacttccg cgctggacaa agagtggatg
    11221 attgatggac gcgggattaa taacgtctgc gatgtgctga tgtatctcga agatgacgat
    11281 acccgtgatg tcggtgtgat aatgacgtta ccgctctttt tcccttttct ctggttcgct
    11341 ttgtggcgaa aaaaacgcgg ctggtttatg tacgccaccg cgctggctat tttcggctat
    11401 tggctgtggc agttttttct gcgctatcag ttttgcttat aagccggatg ggttcatccg
    11461 gccacaaaga ccttacaaac ttcccgcctg acgacctttg cccgcagaag ttaatgttct
    11521 ccccgtttgc ttaccattca ggctttccaa acgcatctgg aagggcggga atggcatatc
    11581 aataccgtgg gcatggaatc ctgccagaat tagctggtga atctcatggc gtagtggcat
    11641 acggtgaccc atctcagcgg cgtaaatacg cagctcgaaa atttgaatcc cctgttgcag
    11701 atccaccagg aagacttccg gtgctgggtt atcgattacc agcgagcagc gacgcgctgc
    11761 ggtgagcagg atttccgtca cttcttcact gttggcatcg gcaggggccg gaatcgtcaa
    11821 caccacgcgc gtgaccgagt cagagagcga ccagttgata aactgctcgg taataaacgc
    11881 cttgttcggc acgataattt ctttgcggtc ccagtcgctg atggtggtgg cgcgggtgtt
    11941 aattttcgtc acactaccgg taagatcgcg gatcgttacc gtatcgccaa tgcgaatcgg
    12001 tttttcgaac aggataatca ggccagagat aaagttggcg aaaatttcct gcaaaccaaa
    12061 accgagacca acaccgagcg cggcgaccag ccactgcaat ttcgaccact caataccaat
    12121 catcgagaag ccgaccagcc cgccaatcag catcagcaga tatttggtga tggtggtgat
    12181 ggcgtagccc gtacccggcg ttaaatccag gtgctgcaga atcgccagtt ccagcagcgc
    12241 gggcaggttg cgcaccagct gcgtggtgat gataaacacc agaatggcaa tcagcaccgc
    12301 accgagggta atcggctcca ggctttctac gccctgtacc gtggaagtga catcccacag
    12361 cgaaatattt tcgaggaagc cgaaagcgga atggatttct gaccacagca caatgaccga
    12421 aagcagggcg atcaacatca aaattgagcg caccagccgc aaggattgcg cactgatggc
    12481 atcgagatcg acttcgcttt catcgacttc aattgctcct tccgggctac tgtgatgatg
    12541 cgcttcctct tcgccacgcg cacgctgcgc taacatctct gcccggcgat gcttcgcccg
    12601 gtcaaacgcc agcctgcggc gctggatcag catccagcgg cggataacgt gataaaccac
    12661 cagtagcagg aaccagatgg caaccgaggt ttcaagcctc gctaacagcg cctgcgccgt
    12721 tgccagataa cccaccgccg acgccagaat ggcaaccagc ggtgcgccaa tcatcatgtt
    12781 ccacagcata tggttggtaa tgttgtcgcc gctgccttct ttgttgagat acagcgggat
    12841 cccggccttt ttcagactga gagtgaccac cgccagcgca ccgcaaatga ggataaagca
    12901 aagccgtccc agcgaaccgg agaattcacg gtcgtcgagg ttatcgaaca tcatcagcgc
    12961 cataatcagc ggcacaataa gcccgatgct catcaggtag tagcgcatcc cacgggaaac
    13021 ccgttcgcgc ggccagccaa aatgagcgat aaacaagccg ttcgggcggg caaaggtggc
    13081 gcaaatcatc actacccaca gcagcggcac ggtggctgtt acgccatcac caatcgcgac
    13141 cgccagcgga taaggccacg cctcgcgcaa gccgtaaccc agcgtcatcc acagcacagg
    13201 cagcggtgac gcgacgagaa tcgaccagaa aagagtgcgt aacgtcagcc agaagtgatc
    13261 ctgagtcact ttgccgactt tcgccgccga acgttcaaga aaacgggtga aatagcggcg
    13321 cgagtaaata ctgcaaccga ccagaatcaa cgcgccaagc agcggcaaaa tcgtctcttt
    13381 actggtcagc atcattacac tggctttgcc caactgactg aaggtgtcca gcgaaatgag
    13441 acgacgcaga tcctgggcga tttccagcgg ccaggcgatg gtcatcgggc gcacgtcaga
    13501 ggtccagaac agatagcggt gcgtcgcttc attcacctct ttcagcgcat cctccagttg
    13561 cccgttggag actttcagct tggtcagttc cagtagtagc gtgtctccac cctgcaacaa
    13621 tgagttcagc aactcacgct gagtgcgtag ctgtgcttcc agaatacggt tttgctcggc
    13681 ggtcagcggc tgaccgtcgg cctgatggat ttgccgtagc agcggctgtt tatttagcag
    13741 atcctcataa cgtaaccgtt gcacacgcaa ctgcgccatt tcggtatcaa gctgttgtgg
    13801 ttttggcatt tccggtagcc gtgccacctg cgcccgcaac gcttcgccga gcaggttgga
    13861 cgagcccagc cattgcgact gttcacgcag cgtattcaac gcctgccgga cctgtaacgt
    13921 ctggctggca gcctgacgct gttgcgaggc aacgagatcc atccgctgcg cctgttgatt
    13981 cagcgctgcc gatagttcgc ggttaatttt gaattgcgcg acgatatctt tcggcaaatc
    14041 ggcactgttt tctgccagta attcggtact ttccagcgcc cgctccgcct caagctgacg
    14101 ttggctgttc aactgattac gtaaggcctg caaatacgca tccagttgct ggctctcttt
    14161 ttccgccagc tctgagcgta agcgcgctaa ttcctgacgg ttattggcag acagctgcgc
    14221 cagttccagt tcatcgacga gcgctttcag gcgtgcggag tcagactgca acgcgaaatt
    14281 ttgtgcctga ttgagcgggg aattgccggt aagcgtcccc aggcggcgct cgatttcatt
    14341 taactgacgg cgggcatcgg tttgctgttg cggcagttga ttcagcgaat cggcaatctc
    14401 gcgggcgcgt tcctgctctt gctgggcctg acggctttta tccagcaact ggctgctgac
    14461 ctggagaatt tcctgattca gcgcgtcggt agacattccc ggtgacacgc tgcgcggttc
    14521 gtcacgcatg ttgtttaatt gtgcgcgcag agtagcggaa agtttcgggt aattatcaat
    14581 gacttcttga tattgcttga tgcgctcaag ggaacctttt cgttcctcaa gcgcatttaa
    14641 ggcggactgg agcgcctcta cgacttccgg ctgcgcgggt ttcgccgctt ttgcctgctc
    14701 cagttcttgt gagatttgtt tgctatcggg ggccgtcgcg gcgtacgccc cccaactgag
    14761 gcaccaggcc atcagaaaag tgataatcag gcgcacgtca gcgtttcctt tgatggatta
    14821 gacctggtct ttgttgtcgt caaccaatgg gttggcgtcg tgttctgctt cgatctcttc
    14881 agcaggaagc ggggccggtt cagcgtctgg tgtaacaaag gtttcggtag atactgccag
    14941 cggctggcca attttcgtga cagacaggct ttccagttgc tcaaccagat tcactttacc
    15001 cggtgcaaac aggttgataa cggtggaacc gagtttaaag cgacccattt cctggccttt
    15061 cagcagtgcc acagaaccgt cgttttcccc ggcaggccag gtccagcgct tgatgatacc
    15121 ttcgcgcggc ggcgtaatgg tgcccgccca gaccgtctca atgctgccta caatcgtcgc
    15181 cccgaccaga atctgcgcca ttgggccaaa ttcggtatcg aacaggcaga tcacgcgttc
    15241 gttacgggca aacagattcg gcacgttctg agcggtgaga tggttaacgg aaaagagatc
    15301 gcccggcacg tagatcatct cacgcagaat accgttgcac ggcatgtgta cgcggtggta
    15361 gtcacgtggg gagaggtaag tggtcacaaa cgtaccgttg cggaacaggt ccgccatcag
    15421 atagttgcca gccagcaggg cttcgaggct gtagttgtgg cctttggctt gcaggatttt
    15481 atcttcttcg attttaccca actggctgat aacgccatcg gcaggcataa ccagtacatt
    15541 cggatcggta tcgattgggc gtacttcgtc acgcagcgga cggacaaaga attcgttaaa
    15601 ggtgcggtag ctggcggtgt ccggcttttg cgcctctttc atatcgacct tgtagtattt
    15661 aacgaacaga tcgataacca gttttgtcag ccatcctgcc cgcttgcttg cgccccaacc
    15721 cgccaggcga gtaagccata gtttcggcag aatgtactgt agcgaaagtt taaatgaatt
    15781 taacaaggta gcctccaggc cattgttttg tcgttcctga tccggcctac atgccggatc
    15841 ctgaaaaaaa ggggacgatt ctaacgacgg ttagcttaat tgtcagtcat ccgtatcaga
    15901 aaagttttta cgcgttttta cctgcgccat gctttccaga atacggtgat agttttcgaa
    15961 acgggtttcc gcgatttttc cttcctcaac cgcttcccgg atagcgcagc ccggatcggt
    16021 atcgtgtttg caatcgcgat atttacacag acctaagtag tcatggaatt cgacaaagcc
    16081 ctgagtgatt tgttccggct ccaggtgcca gaggccgaac tcacgcactc ctggggagtc
    16141 aatcacatca ccgccgtgcg ggaagtgata cagccgagcg gcggtcgtgg tgtgctgacc
    16201 gagacccgag ttgtcagaga catcgttggt caggatctct ttttgcagac ccagcaatgc
    16261 gttcagcagg ctggatttgc caacgccaga ctgcccggca aaaatgctga tgcgcccggt
    16321 caacgcctct tccagcggtt tcagcccatc ctgagtatga ctggaaacca tcaatacgcg
    16381 ataaccgata ttgcggtaga tatccatctg ctcgttgacg aatgccatgc cttcgtcgtc
    16441 cagcaggtct atcttgttga gcacaataat cggctcaatc tgcaaggttt cgcaggccac
    16501 caggtaacgg tcgataatat tgagcgacag ctccggcaaa atggcggaga caatgacaat
    16561 ctggtcgatg ttggcagcaa taggtttcac accgtcgtaa aaatccgggc gcgtcaacac
    16621 tgaggtgcgc tcatgcaccg cttccacgat ccctttgaca tttacgcctt ccgccgctgg
    16681 tttacccgga cgccagacta cgcggtcgcc ggtcaccaac gaacggatgg tacggcgaat
    16741 attgcagcgg tgaacgtcgc catcggcgga ttccacatca gcgtgcatac caaagcggct
    16801 gatgacgata ccttcatcag gctcgccaaa cagattgtcg tcgtagtcgg gcttctcctt
    16861 agacgtttta agacgacgct ggtgattggc gttcacgcgg cgctgctggc ctttggagag
    16921 tttattttta ctcaatcgta cagactcctg gtcgcccctg atgggcaaaa catctatgat
    16981 acacgcaatt gtggatcaat atagtcactg tgaatgggtg gaaaatagca tgagtgccaa
    17041 tgaaaacaac ctgatttgga tcgatcttga gatgaccggt ctggatcccg agcgcgatcg
    17101 cattattgag attgccacgc tggtgaccga tgccaacctg aatattctgg cagaagggcc
    17161 gaccattgca gtacaccagt ctgatgaaca gctggcgctg atggatgact ggaacgtgcg
    17221 cacccatacc gccagcgggc tggtggagcg cgtgaaagcg agcacgatgg gcgatcgtga
    17281 agctgaactg gcaacgctcg aattcttaaa acagtgggtg cctgcgggaa aatcgccgat
    17341 ttgcggtaac agcatcggcc aggatcgtcg cttcctgttt aaatatatgc cggagctgga
    17401 agcctacttc cactaccgtt atctcgatgt cagcaccctg aaagagctgg cgcgccgctg
    17461 gaagccggaa attcttgatg gttttaccaa gcaggggacg catcaggcga tggatgatat
    17521 ccgtgagtcg gtggcagaac tggcttacta ccgcgagcat tttatcaagc tgtaaaattt
    17581 ttcctggtca cgtaagcgcc ggaatccact tgcggcggcg tgattttgac gctaaattga
    17641 acgtcttgct gataatttca gcacttgaga taaaaactca aaaaaaattt tttagggggg
    17701 ttgcagaggg aaagatttct cgtataatgc gcctcccgta acgacgcaga aatgcgaaaa
    17761 ttacgaaagc aaaattaagt agtacgcggg aatagctcag ttggtagagc acgaccttgc
    17821 caaggtcggg gtcgcgagtt cgagtctcgt ttcccgctcc aaaatttgaa aagtgctgca
    17881 aagcacagac cacccaatcg cagtatttcg aattgcgaca aggcggcaac tgaacgaatc
    17941 cttgggaact tacaaaagta agtgactaag gtgagtgaag gcagccaagg cagtagcggt
    18001 tcgaaagacg aagattaagc gggaatagct cagttggtag agcacgacct tgccaaggtc
    18061 ggggtcgcga
//
LOCUS       DAZKGF010000097        18000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZKGF010000097 DAZKGF010000000
VERSION     DAZKGF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.097"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(82..2529)
                     /gene="glgP"
                     /locus_tag="ACHOQV_003126"
     CDS             complement(82..2529)
                     /gene="glgP"
                     /locus_tag="ACHOQV_003126"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFP9631460.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2548..3981)
                     /gene="glgA"
                     /locus_tag="ACHOQV_003127"
     CDS             complement(2548..3981)
                     /gene="glgA"
                     /locus_tag="ACHOQV_003127"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFP9631461.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3981..5276)
                     /gene="glgC"
                     /locus_tag="ACHOQV_003128"
     CDS             complement(3981..5276)
                     /gene="glgC"
                     /locus_tag="ACHOQV_003128"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFP9631462.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5294..7267)
                     /gene="glgX"
                     /locus_tag="ACHOQV_003129"
     CDS             complement(5294..7267)
                     /gene="glgX"
                     /locus_tag="ACHOQV_003129"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFP9631463.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7264..9450)
                     /gene="glgB"
                     /locus_tag="ACHOQV_003130"
     CDS             complement(7264..9450)
                     /gene="glgB"
                     /locus_tag="ACHOQV_003130"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFP9631464.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVRTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNVGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9723..10826)
                     /gene="asd"
                     /locus_tag="ACHOQV_003131"
     CDS             complement(9723..10826)
                     /gene="asd"
                     /locus_tag="ACHOQV_003131"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9631465.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            11018..11611
                     /gene="yhgN"
                     /locus_tag="ACHOQV_003132"
     CDS             11018..11611
                     /gene="yhgN"
                     /locus_tag="ACHOQV_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFP9631466.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11668..13008)
                     /gene="gntU"
                     /locus_tag="ACHOQV_003133"
     CDS             complement(11668..13008)
                     /gene="gntU"
                     /locus_tag="ACHOQV_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP9631467.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(13012..13539)
                     /gene="gntK"
                     /locus_tag="ACHOQV_003134"
     CDS             complement(13012..13539)
                     /gene="gntK"
                     /locus_tag="ACHOQV_003134"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9631468.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13678..14673)
                     /gene="gntR"
                     /locus_tag="ACHOQV_003135"
     CDS             complement(13678..14673)
                     /gene="gntR"
                     /locus_tag="ACHOQV_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFP9631469.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14897..15592)
                     /gene="yhhW"
                     /locus_tag="ACHOQV_003136"
     CDS             complement(14897..15592)
                     /gene="yhhW"
                     /locus_tag="ACHOQV_003136"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFP9631470.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15715..16752)
                     /locus_tag="ACHOQV_003137"
     CDS             complement(15715..16752)
                     /locus_tag="ACHOQV_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9631471.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            17086..17574
                     /gene="yhhY"
                     /locus_tag="ACHOQV_003138"
     CDS             17086..17574
                     /gene="yhhY"
                     /locus_tag="ACHOQV_003138"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP9631472.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            17811..>18000
                     /locus_tag="ACHOQV_003139"
     CDS             17811..>18000
                     /locus_tag="ACHOQV_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFP9631473.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNSITNTGLGSQFHGITFC"
BASE COUNT         4448 a         4861 c         4482 g         4209 t
ORIGIN      
        1 ttgtttattg tgtatagaaa taccgtgagt tccactctgt attacttcag cggaacacac
       61 ggtatttagc catatttgaa cttacaatct caccggatca atatgccaga tatgatcggc
      121 gtactctttg atcgtacggt cagaagagaa gtagcccata ttggcaatgt tcagcatcgc
      181 ttttgcggtc cactcttcct gacgctcgta gagttcgtcg actttatcct gacaatcgac
      241 atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca gcgaatcgac
      301 cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga tttgcgtcag
      361 cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc cctgacgacg
      421 cagttcttcc acttcttccg ctgtgttacc aaagataaag atattgtcag caccgacatg
      481 atccagcatc tcgacattcg caccgtccag cgtaccgata gtcagcgcgc cgttaagcgc
      541 aaacttcatg ttactggtgc cggaagcttc cgtccccgcc agcgaaatct gttcagacag
      601 atcggccgcc ggaatgatca actgcgccag gctaacactg tagttcggga tgaacacgac
      661 tttcagcttg tcgccaatct gcggatcgtt gttgatcact ttcgctacgt cattgatcaa
      721 atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccaccaa aaatattcac
      781 gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg tgatcacgtg
      841 caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgca catcgaacag
      901 cgctttcgga tttaccacca cattcagctg ctgggcgata tactccgcca gacgcttttt
      961 gttctccagc ttcgcctgat gcaccgcgtg attaaccatt gggaaatcac agtgttgttg
     1021 cagatcatta agcaggctaa ggtcggtgcg ccagttgcgg cccaggtgtt cgtccaacac
     1081 ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac cgttggtgac
     1141 gttggtgaaa cgacccggga agattttcgc aaagtcggca aatagcgatt gcaccatcag
     1201 gttagagtgc agctccgata caccgttaac tttgtggctc accacaaccg ccagccaggc
     1261 catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca gcagatcggt
     1321 atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt caaagatgat
     1381 ctgcaggtga cgcggcagaa ttttacccag catatcgact ggccaggttt ccagcgcctc
     1441 gctcattagc gtgtggttgg tgtaggagaa gacctgacaa cacacttcaa acgcgtcgtc
     1501 ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg acagcaccgg
     1561 atgagtatcg ttgaggtgaa tcgcaatttt atccgccagg ttatcgtagg ttttatgcaa
     1621 ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt attcctgacg
     1681 cagtcgcagc tcacgcccgg agtaggtgga gtcgtccgga tacagtacgc gagatacgtt
     1741 ctcggagtgg tttttatctt ccactgccgc gaagtagtcg ccctggttga atttaccgag
     1801 gttaatttcg ctactggctt gggcactcca caaacgcaac gtgttggtcg cgtcggtgtc
     1861 gtaacctggg attatctgat cgtaagctac tcccagaatc tcttcggttt caatccagcg
     1921 cgttttttta ccttcctgct gaatgcgacc gccaaaacgg actttatagc gcgtgttgtg
     1981 gcgtttgaat tcccacgggt taccgtattc cagccagtag tcaggcgact ctttctggct
     2041 accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt aaccgcgccc
     2101 cggcaaccct aacgtcgcca gagaatcgag gaagcaagcc gccagacggc ccaggccacc
     2161 gttaccgagg cccgggtcat tttcttcatc aatcagctct tcgagattta atcccatcgc
     2221 ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt tggaaagcgt
     2281 acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt gcgacaactg
     2341 ggcacggttt gagcgtaacc agcgctccac gagacgatcg cgcacagcaa ataacgttgc
     2401 gttcagccat tcatgtttat tggcgacgac cggatccttg ccaatcgtaa acatcagctt
     2461 gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat atgtaaacgg
     2521 agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct cacggtacga
     2581 cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca caaaccgcca
     2641 cagtgaagga cgggaccaca gtacaaaagc acgtcgaata gcccgtaaca gcgaccaggc
     2701 attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct caagagaaca
     2761 gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg taccgtactt
     2821 caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca gaatgacgtc
     2881 cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa cgcccacctg
     2941 gccggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat cgcccgcgcc
     3001 gagtagcgcc agctgcccgc cctgctccag aagaccaggt aaggcttcca gcaccaggtc
     3061 gagacctttc tggctagtca gacggctcac cactgcaaaa agcggcactt tatcgtcaac
     3121 cttaagcccc attgcgattt gtaactggcg cttattttcc gctttatctt ccaacgtatc
     3181 gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt tctcgtccac
     3241 gccgttcagt acgccggaaa gacgcccttc acggtgacgc tgttgcaaca gaccttccat
     3301 accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttgggctga ctgccgtaat
     3361 gtgatcggca tagtacagac cggccttcag aaaagagatt tgtccgttga attccagccc
     3421 atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg cataaaacat
     3481 gccttgatag gccaggttgt gcacagtaaa caccgacttc gccggacgcc cgcgcgccgc
     3541 cagatacgca ggcgcaaggc ctgcatgcca gtcgtgcgca tgcaccacat caggacgcca
     3601 gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg caaaacgcaa
     3661 tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg gacgatcata
     3721 gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga acaacagcgt
     3781 gatatgtccg gcgaaggtat cacgacggga tactacctgc gcatcggtca cgccacggcg
     3841 aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga tttgtgctgc
     3901 gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga acatctctga
     3961 acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg tagcatttcg
     4021 cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc ttcctctgcg
     4081 ttttcaccaa tcaccatgcc ttccggaata acacaagcac gatcgatgac gcagcggcgc
     4141 agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc aatgttgcag
     4201 aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga accggagatc
     4261 acacaaccgc cggataccag tgagttaagg gtcatcccat ggctaccgga gcgatcctgc
     4321 acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt gcgatcgtac
     4381 atatccagtt ccggcaccac agaggccaga tcgaggttcg ctttccagta agcttccagc
     4441 gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca agagagcggg
     4501 aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc tttgccaaag
     4561 tcgtggctgg agttctcatc gcgatcgtct tcttccagca gttcatacag atagtcggcg
     4621 tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg cattgacggt
     4681 gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac cgccataacg
     4741 ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca acgtgcgcct
     4801 ttttcgacgt ggtcgataag catacgcgag tagtcttgct tgtagatatg gtcgcccgcc
     4861 aggatcacca cgtattccgc tttataacgg cggataatat cgaggttttg ggtaactgca
     4921 tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg cagcagatcg
     4981 acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg ctgcaccaga
     5041 gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccggagtt gatgcagtta
     5101 gacagcgcaa agtcgataat gcggaactta ccgccgaagt gtacggccgg ttttgctcgc
     5161 ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag ggcaacagat
     5221 ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact aaccatgact
     5281 aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc tgccagacag
     5341 ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac tccccagcag
     5401 gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag cgatccgaga
     5461 gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa ggttgagcat
     5521 atcgatttag ccaacggaca ttgccgtcgc cttcttccca ccagcgattc tccaccaaag
     5581 cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa ccactgcttg
     5641 cctgcgacca gtccaaccag gttaattggt tatcctgaca gtaggcattg ttattgccat
     5701 gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtaccctgg gagagcaaca
     5761 acgttgttaa cagggcgtga atgctgtcgc gacgccgttc aaccaggtca agagtaccgc
     5821 ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg cgattttctt
     5881 ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc gtaaaaccgt
     5941 catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt ttaaaaacat
     6001 cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa tgaagccaga
     6061 aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc gggaaatttc
     6121 ccacctgata accaccagga gcaatatccc acggttcagc aattaacttc acctgcgaga
     6181 gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac tctggcgtac
     6241 ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt tctacccaat
     6301 aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg agcgtgttgc
     6361 cacaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag ctacggttgt
     6421 cgatcccacg cagagagaat aacgggccgt cgaggtccag ttccgcacta tggttgagca
     6481 cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca tcgcgaaact
     6541 catccagcgc cgtttctggg gagcaggcat acgccggatg cagcgcaaac atcgccaccg
     6601 ggttataacc ccagtagtta cttagaccca tacgttgcag gcgtggttcg ctggcaaact
     6661 gcgccaccgg cagcagttcc agcgcggtaa tgcccaactg tttcaaatag ttgatcatca
     6721 ccggatgccc cagggcttta taagtgccac ggatctcgac cgggatctcc gggtgcaggt
     6781 acgttaatcc tttgacatgg gcttcataaa tgatggtact gccccacggc gtgcgcggcg
     6841 gagcatcatc ctcccagtca tagtgatcaa ccaccactac gcatttcggc gcaatggcgg
     6901 cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta tctttaaatt
     6961 ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta aagcgatgcc
     7021 cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc ggacgcgcat
     7081 ccggcagata accgtgccaa atgtcgccac tgtgccctgg caagtcatag cgatgttcct
     7141 ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag aaaagtgtga
     7201 agttgacgcc ctgaccgtcg taatgcgcgc cgaggggtgc gggtttgcca atggcgagtt
     7261 gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagtg tcaggcttaa
     7321 tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc cattgccgac
     7381 attactgccg tgatagtgca tggaatcggt attgaggatt tcacgccatt tgcccggctg
     7441 gttaatgccg aagcgataat catgacgcgg caccggagta aagttactgg caacgatgat
     7501 ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt tgtcatccac
     7561 caccagccat tcaaaaccgt acgggtcaaa atccagttca tgcatcgctt tatggtggcg
     7621 gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt tatcgccgcc
     7681 ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct gggcaaattc
     7741 gttccccatg aacagcagtt tcttgcccgg gaatgcccac atccagccgt agtaggcgcg
     7801 caggttagcg aatttctgcc atgcgtcgcc cggcatccgg tcgagaatcg attttttacc
     7861 gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt tgtagagcat
     7921 cccgaaggtc agtttatcgt gatgatactg acgataaacc gggtcgagct tcatgtagtc
     7981 cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac cgcccatatc
     8041 ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca ccgcaccgga
     8101 aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt caagattctc
     8161 gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt cgcgataaat
     8221 cattgacgct accgcatcga cgcgcagcgc atcaatacca aaacgttcaa tccagtaaag
     8281 cgcgttaccg acaaggaagt tactgacttc acggcgacca tagttgtaga tcagcgtgtt
     8341 ccagtcctga tgatagcctt cgcgcggatc gctgtgttca tacaagttcg tgccatcaaa
     8401 ttcggcaagc gcaaagtcat cggtcgggaa gtggcctggc acccagtcga gaatcacgtt
     8461 cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag taccaaaacg
     8521 gcgggtcggt gcatacagac cggttggctg ataaccccaa ctgccatcga agggatgctc
     8581 gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag gcaccagttg
     8641 atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac gccaggagcc
     8701 cagatgaact tcataaatag agattggcgc atcaaactga ttcgcttttt tgcgctcttc
     8761 agtccgtaca accttttccg gcagcccgca aataagagac gcggtttccg ggcgcatttg
     8821 cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat caatcatctc
     8881 gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga tgccgctctc
     8941 tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc caaccaccga
     9001 gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc catccatagt
     9061 atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag ataatagcca
     9121 ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca ggttttgctg
     9181 accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg gaatgacgcc
     9241 gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc cggttttcgg
     9301 ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca gtcccgcggt
     9361 ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa ttagcgcgtt
     9421 aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt gtgtcacccc
     9481 atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg gcgcagcact
     9541 ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact catgtattca
     9601 gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg aaacatctgc
     9661 gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt aatgaataaa
     9721 gattacgcca gttgacgaag catccgacgc aacggctccg cggcccccca cagcagctgg
     9781 tcacccacgg taaaggctga caggaactcc ggtcccatat tcagcttacg caggcggcct
     9841 accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat agtgatttcc
     9901 cgatcgttcg gaacgacttt cgcccacgga ttgtgtgcag ccagcagttc ttccacggtc
     9961 ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca gcgcaatgcc
    10021 ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag gatcttgttg
    10081 gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg tttgtcgatc
    10141 cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag ctcaccgcta
    10201 cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg ggtcgcgagt
    10261 tcatctgcca catggccata cagatggccc atctgggtta ataactcacg catatgtcgc
    10321 gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac aagatcattg
    10381 gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc gccaacaaaa
    10441 gtcctgatgc cattatttaa tccatcggta atgacgtcct gattgacggg gtcaagaatg
    10501 atgatggcat catctttcat gcgcagagac gatgctgcgt caatccagta accttgccat
    10561 ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg acaggtcaca
    10621 atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt gccagtggtt
    10681 ccgccaaaag acggcgcagc ctgaccaagc tgagaagtag aaaagaagac agggcgaatg
    10741 gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac cataccgcgc
    10801 cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt gtgctgtata
    10861 aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca aagtcgcaag
    10921 tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct ttgttagcac
    10981 gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca tttctgcagc
    11041 agttttattg atcctgatta tggatccgct cggaaaccta cctattttca tgtccgtact
    11101 gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc ttattgctct
    11161 cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc tcagcctgcg
    11221 agcagaaacc gtctccattt ctggcggcat cattctgttt ctgatcgcca ttaagatgat
    11281 tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc cgtttatcgt
    11341 gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga tgttgttgtc
    11401 tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg cctggggcgg
    11461 cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg agaaaggggt
    11521 gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaaccc agatgttcct
    11581 cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa agagcccggt
    11641 agcgtcagaa ctaccgggta aacaaactta actcaacagc tggaacgcaa tcatcccaac
    11701 gatggcaccg acagtgccga ggatggtttc catcatggtc caggttttca gcgtttcggc
    11761 ttcggtcgcg ccggtaaatt taccgaacaa ccagaaaccg gcgtcgttaa cgtggctgac
    11821 aacaatcgaa ccaccggcga tacaaatcga cagcgccgcc atttgcgcac cagagtagtt
    11881 cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta cggtggcaga
    11941 cccctgaatg atgcgcactg cagctgccag cacgaagcag gtgatagcaa tcggcaggcc
    12001 catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca gcacctgttt
    12061 gaacacgccg cccgcaccaa tcaccagcag aatgatcccc gccggttgca gcgcgtgacc
    12121 gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc cataaatcgc
    12181 taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga accattcgta
    12241 agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca ccagcaccag
    12301 cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt cgccgagatg
    12361 cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga agttacccca
    12421 cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca ggatcatcca
    12481 gccaaagtcg gcgttcatct gcgatgccag cagcattggc gctggccccg gcaccaggaa
    12541 cgcagccgct gccgccacgc ctgcaaataa tgggattacc agcttcacca ggttcgtacc
    12601 ggtatgacgc gccattgaga aagcaacgct aatcagcaga acaatcgcca cttcaaagaa
    12661 cagcggtagc gcacaaacca gccccgcaag gccaatggca taatgcgcgc ggctgtgacc
    12721 gaaggattta agcattttga cggcaatctg atcgactgcg ccggtttcat gtaagatctt
    12781 gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc ctcccatccc
    12841 tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc cagcccccat
    12901 ggacaccacc attaaagcca ggaaagcgtg catacgcgcc ttcatgacta aaaacagcag
    12961 cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta cttatttgcc
    13021 ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat cgatatccac
    13081 caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact gcgtcaccaa
    13141 catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cggctttcaa tcacatcaaa
    13201 atcacctttc aggtagatga aagagagatt cggattacct tcacgcagca agtcgcgata
    13261 gtgttttttc aatgcagaac agacgatcag tgacacttta ttagtgcgct gcatagcaaa
    13321 cgcggcgtcg ttcagcgcct gcagccacgg tttgcgatcg tcatcattca gcggttcgcc
    13381 agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa gaaacgcggc
    13441 atgaagttga tgcgccactt cacttgcgac cgcagatttg ccgctgcccg atacgcccat
    13501 caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc cacaatacaa
    13561 gaattaaaaa tgttacgggt aactgttatc ggtaacattg tccagccgga caatatcaga
    13621 agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt tgtgggctta
    13681 aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg tcacagattc
    13741 gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct cacgcggcgt
    13801 caggacgctc gccaaccgtg gctccatcac ctggccaatg tcatgaccgt ggaaaccggc
    13861 aatcgccata tcgtcaggaa ctttcaaccc cagacgctga cattcaaacg ccgcgccgac
    13921 cgccaggtca tcattagtac agaacacgcc atccagctgc ggatattccc gccgtgcctg
    13981 gcgaatcagt tcaataccgg aagaataaga agaagattgc tcaaccatca cgctatacgg
    14041 caccaggcct gcatccagca tcgcctgttc atatcccttc tgtttgatga tagtacgttc
    14101 gtcgagacgt gcgccgagat aggcaatgtg gcgatgcccg cgagcaataa tggctgtggt
    14161 catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc aaggcgactt
    14221 gctgtccatc agttccacca ccgggatccc cgccacttca atcatcttta aggtgcgcgg
    14281 cgtgtgggta cgttcggtga ggatcagtcc gtcgatattc catgagagca tggattcgag
    14341 acgttcttgc tccatttccg gtttataacc gtagtgggcc agcatggtct gataaccgtg
    14401 cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt tggtgagaga
    14461 aggtaacagg acgccaatcg cccggctggt ggcgttagag aggatatcgg gtgcgcgatt
    14521 gggaatataa cccagttcat caagagcggc ggcaatcttg ccgcgtagag cgacggaaac
    14581 ctgctccggg ttacgcaaaa agcggctgac cgtcattttg gtcacgccta cacggtcagc
    14641 cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa agagatagat
    14701 tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc ttgtttacaa
    14761 ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg caaaaccccg
    14821 gaagcgtaca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag gcaggcttca
    14881 aagatgccgt tgagctttaa accggcggca gatcgaacag taacacttcg ctgtcgctat
    14941 ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg atcgaggctt
    15001 tcacgccgtt aatggtgaca ttgcctttca ccacctggat ccagacgcgg cgttcagcgg
    15061 caatctgatg caccgactgc tcatctttca gcaacgccca gcggtacagt tccatatcct
    15121 gatgcacttt caatgaacca tcacgcgcat ctggcgaaag caccagttgt ttgccctgca
    15181 ctgcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc gttttcttcc ggcatgatcc
    15241 agatctgata caggtgcaga cgctcggtgc tgcttgggtt gtactctgag tgacgaatac
    15301 ccgtacccgc actcataatc tggaactcgc ccgccggaac ctgctcttta ttgcccatgc
    15361 tgtcctggtg ctcaacagta ccttccagca cgtaggtcaa aatttccata tctttatgcg
    15421 ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg cgcagcgcgg
    15481 agaagcccat aaagttcgga tcgtaatagt tggcaaaaga gaaagtatgc caggagtcca
    15541 gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc atttgtattc
    15601 gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga tgatagaggg
    15661 tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc aaacttactt
    15721 cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga tttccagatt
    15781 ggtaagaact tcagattcct tgacgtaatt tggcgcaccg ttggtgatgg tttgatacaa
    15841 cgcatcataa acgcgcccgt aatcgcccat ctccggcttc atctcttctc tgaccgtcac
    15901 accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga atcccggttc
    15961 gcccggcata atattagcct tgaggctggt ttcctgctgg tcgataccat atttaataaa
    16021 cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca gatggctggt
    16081 tttgacgatg gctttcaagt cgccataaaa cagttgcgct tcaaaggtgt cgtcaggatt
    16141 ggctttattg cgcaggctgc ggatgtcata agcgacgtga tccgggcgac cgaacagaga
    16201 aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct gcggcaaccc
    16261 aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt caacaatctc
    16321 tcccaacttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat caaagcgacg
    16381 attctgatac ggcgtgacgg tcagcccttt gcttttcgcc agcgcaaaca gctccttcgc
    16441 ctgggcaagc gtcggggtga acggtttttc gaccagcaca tttttcccgg cttccagcgc
    16501 gcgtttcgcg tattcgaaat ggctgtccgc gtgggtgcag acgacaacca gcttaacatc
    16561 gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt aaatgggagc
    16621 ctgttcttcc ggctttgcat ggcggcgaaa aatatgcgcg acatgccagc tatccttgcg
    16681 gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa taaaggcgca
    16741 gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc gcaaagcgga
    16801 cgtggttcct actggagtgc gcataacgaa cacaagcact tcccgaggat aaattgagaa
    16861 cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa gcaatgtgag
    16921 caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgagaca ataataatca
    16981 ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc gcattcgaaa
    17041 gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag tgagatagtg
    17101 atacgccacg cagaaacacg ggattacgag gccatcaggc agattcacgc ccagccggag
    17161 gtgtattgca acacactaca ggtgcctcat ccctccgatc atatgtggca ggaacgactc
    17221 gccgatcgcc ccggcatcaa gcaactcgtc gcctgtattg atggaaccgt agtaggtcat
    17281 ctcaccattg atgtacagca acgcccgcgc cgcagtcatg ttgccgattt tggtatctgt
    17341 gtcgactccc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga gatgattgaa
    17401 atgtgcgaca actggttgcg ggtagatcgt attgagctaa cggtgttcgt cgataacgcg
    17461 ccagcaatta aggtctataa aaaatttggc tttgagattg aagggactgg caagaagtac
    17521 gcattacgta atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa gtaagatagt
    17581 gccctttttc tgagatggaa aaagggtgtc attcaaaatc ggcatacctt cctttaaggt
    17641 atttattttc ccaatacata tattgagatt taactcataa ttacttccta aagtgtaata
    17701 ttttattttt taatatatac gcctacaatt tcctggcgta aataaataac aattaacaag
    17761 cataatattg ccattgataa aatagcatgc cataaaagga cttttcaggg atgagtaata
    17821 ttgtttacct gacagtaacg ggagaacaac aaggaagcat ctccgcaggt tgtgggactt
    17881 ctgagtctac aggtaatcgc tggcagagcg ggcatgagga tgaaatattt acattctcac
    17941 tcttaaatag tattactaat acggggcttg gttcacagtt ccatggtata acattttgta
//
LOCUS       DAZKGF010000098        17925 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZKGF010000098 DAZKGF010000000
VERSION     DAZKGF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.098"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(211..1056)
                     /gene="sseA"
                     /locus_tag="ACHOQV_003140"
     CDS             complement(211..1056)
                     /gene="sseA"
                     /locus_tag="ACHOQV_003140"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFP9631474.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPEK"
     gene            1263..6224
                     /gene="yfhM"
                     /locus_tag="ACHOQV_003141"
     CDS             1263..6224
                     /gene="yfhM"
                     /locus_tag="ACHOQV_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFP9631475.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQEFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNSARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGVEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVRFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTTSGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFTLWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDAMRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLSASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            6225..8537
                     /gene="pbpC"
                     /locus_tag="ACHOQV_003142"
     CDS             6225..8537
                     /gene="pbpC"
                     /locus_tag="ACHOQV_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFP9631476.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWMHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLALLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAIPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLAEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDEVGQIATVKFVMQ"
     gene            8686..9117
                     /gene="ndk"
                     /locus_tag="ACHOQV_003143"
     CDS             8686..9117
                     /gene="ndk"
                     /locus_tag="ACHOQV_003143"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP9631477.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            9267..10421
                     /locus_tag="ACHOQV_003144"
     CDS             9267..10421
                     /locus_tag="ACHOQV_003144"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFP9631478.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            10706..11713
                     /gene="rodZ"
                     /locus_tag="ACHOQV_003145"
     CDS             10706..11713
                     /gene="rodZ"
                     /locus_tag="ACHOQV_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFP9631479.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            11740..12858
                     /gene="ispG"
                     /locus_tag="ACHOQV_003146"
                     /gene_synonym="gcpE"
     CDS             11740..12858
                     /gene="ispG"
                     /locus_tag="ACHOQV_003146"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFP9631480.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            12969..14243
                     /gene="hisS"
                     /locus_tag="ACHOQV_003147"
     CDS             12969..14243
                     /gene="hisS"
                     /locus_tag="ACHOQV_003147"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP9631481.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRTLGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            14261..14881
                     /gene="yfgM"
                     /locus_tag="ACHOQV_003148"
     CDS             14261..14881
                     /gene="yfgM"
                     /locus_tag="ACHOQV_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFP9631482.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            14892..16070
                     /gene="bamB"
                     /locus_tag="ACHOQV_003149"
     CDS             14892..16070
                     /gene="bamB"
                     /locus_tag="ACHOQV_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFP9631483.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            16188..17660
                     /gene="der"
                     /locus_tag="ACHOQV_003150"
     CDS             16188..17660
                     /gene="der"
                     /locus_tag="ACHOQV_003150"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP9631484.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEADDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            17723..>17925
                     /locus_tag="ACHOQV_003151"
     CDS             17723..>17925
                     /locus_tag="ACHOQV_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP9631485.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLISKKRVE"
BASE COUNT         4292 a         4423 c         5029 g         4181 t
ORIGIN      
        1 acggcggaaa gggccggtgt cgataatacg ctaaataaac aatgattact ctctttcgct
       61 tgacaaaaag agagttactg gcgagtattg ctttgctgtg aagcacctgc gttgctcatg
      121 caacacaagc caatctgctg gaaaggccgc gaaccagacc agcaataaaa aaccgccaga
      181 ttcggcggtt ttttattgcc tgttaaaagc ttatttctct ggctcaactg gtaaatctgc
      241 tcgtgcgccc cattcactcc atgcgccgtc gtacagtttc acgtttggca catcaagtgt
      301 cgcgagtgcc aacaaaacca cggctgccgt tacaccagag ccgcagctga cgataatagg
      361 tttgtcataa cttacgccgc gaccaaaaaa tatcgcatcc agttcatcgg tcgtttttag
      421 ttcaccttcg cgcaccagtt ccgtccacgg aacatttagt gcaccgggaa tatgtccgcg
      481 acgtaaacct gggcgaggtt catcaacttc tgcgttaaaa cgtgcagccg ggcgggcatc
      541 gataatttgc gccgtatttt catggcttgc caataacaca tcggttactt tcaccacggc
      601 ttcaggatta aacgcggcgt taaactctcc ttccggcagc tctactgcgc cttcttctaa
      661 tagcagatca tcacgctgcc agcctgcaag tccaccaccc agaatcgaca ctttctctac
      721 accaaaggtg cgcagcatcc accatgctcg tggggctgag aaaagattac cttcgtcata
      781 gacaatcagg tgcttatcct ggttaacgcc taattcacgc atcgccacgg cgaacgtttc
      841 cgggcgcggc agcatgtgcg gaagcgggga agtgtgatca gaaagcgctt ctatatcaaa
      901 aaacactgcg ccgggaatat gtccattcag atactcctga gcaacgttac gatcttcctg
      961 tccaggcgac gccatgcggg catcgataat ctgaatttcc gggtcatcaa tatgttcggc
     1021 gagccagtcg gctcctacaa accatgtcgt ggacataggc atctccattt ttgccggggt
     1081 taggctaaat tgtcggatgt ttagtgaaag gcgacaagca aaagaggctg atttggctgt
     1141 ctgagccaca attctcacta aaattcggat tgtatgggcg tgatactgaa ttacataacg
     1201 taaattctca cgcataataa ttattcgacg ttgctaacgg gccattgtgg cagggatgaa
     1261 aaatgaaaaa gttacgcgta gccgcctgca tgctaatgct ggcgctggca gggtgcgaca
     1321 acaacgataa cgcgccaaca gcggtgaaaa aagatgcgcc ttctgaagtt actaaagcgg
     1381 cctcttcaga aaacgtgagt tcagcaaaac tttccgcgcc agagcgacaa aaactggccc
     1441 aacagagtgc cggtaaggcg ctgacattgc tggatctctc tgaagttcaa cttgatggcg
     1501 cagccacgct ggtgctgaca ttctccatcc ctctcgaccc ggatcaggaa ttctcacgcg
     1561 ttatccatgt cgtcgataag aaaagcggca aagtggatgg tgcctgggag ctgtcagata
     1621 atcttaaaga gttgcgttta cgccacctcg aaccgaaacg tgatttgatc gtcactattg
     1681 gcaaggaggt caaagcactc aacaacgcaa ccttcagtaa agattacgaa aaaactataa
     1741 ctacccgaga tatccaaccc agcgtcggtt ttgccagccg tggctcgttg ttacctggta
     1801 aagttattga agggctgccg gtaatggcgc tcaacgttaa caatgtcgat gttaacttct
     1861 tccgtgttaa gccagaatct ctgcctgcat tcattagcca atgggaatac cgcaattcgc
     1921 tggcgaactg gcagtctgac aaactgctgc agatggcgga tctggtttat accggacggt
     1981 ttgatctcaa ttctgcgcgt aacacccgtg aaaaattatt gctgccgctg ggcgatatca
     2041 aaccgcttca gcaggcaggc gtgtatctgg ctgtgatgaa tcaggctgga cgttacgatt
     2101 acagtaatcc cgcgacgctg tttacgttaa gtgatatcgg cgtttcagct caccgttatc
     2161 acaatcgtct ggatatcttt acccaaagcc tggaaaacgg cgcggcccag caaggagttg
     2221 aagtctcatt attaaatgag aaagggcaga ctctgactca ggcaaccagt gatgcccagg
     2281 ggcatgtgca gctggaaaat gataaaaacg cggcattact gttggcgcgt aaagacggtc
     2341 agacaacgct actcgattta aaacttccgg cgctggactt agcagaattt aacattgctg
     2401 gcgcgccagg ctatagcaaa cagtttttca tgtttggccc gcgcgatctt tatcgccctg
     2461 gtgaaacagt catcctcaat ggtttattgc gtgatgcaga tggtaaagca ttgcccgatc
     2521 aacccatcaa gttagacgtg attaaacccg acgggcaggt actcaggagc gtcgttagtc
     2581 agccggagaa tggcctctac cactttacct ggccactcga tagcaatgcg gcaaccggta
     2641 tgtggcatat tcgcgctaac acgggcgata accagtatcg gatgtgggat ttccacgtcg
     2701 aagattttat gccagagcgc atggcgctga atctgaccgg tgagaaaacc ccgctaacgc
     2761 cgaaagatga agtgagattc tccgtggtgg ggtactacct gtatggtgcg cctgctaatg
     2821 gtaatacttt gcaagggcaa cttttcctgc gcccactgcg tgaagctgtg tcagccttac
     2881 ctggtttcga attcggcgat atagctgccg aaaatctttc ccgcacgctg gatgaagttc
     2941 agttgacgct ggatgataaa gggcgtggcg aagtttctac agaaagccag tggaaggaaa
     3001 cgcattcccc attacaggtt attttccagg gtagtttgtt ggaatcgggc ggtcgcccgg
     3061 tgacgcgccg cgctgagcag gctatctggc ctgccgatgc attgccgggg atccgtccgc
     3121 agttcgcctc gaaatcggtt tacgattatc gcactgacag cacggtgaaa cagcccattg
     3181 ttgatgaagg cagtaacgcc gcttttgaca tcgtttatag cgatgcgcaa ggcgtaaaaa
     3241 aagccgtgtc gggcttgcag gtgcgcctga ttcgcgaacg ccgcgattac tactggaact
     3301 ggtcagaaga tgaaggctgg cagtcacagt ttgatcaaaa agatctgatc gaaaatgaac
     3361 aaactctgga tctgaaagcg gacgaaaccg gcaaggtcag tttcccggta gagtggggcg
     3421 cttatcgtct ggaagtcaaa gcgccgaatg aagcggtcag tagtgtgcgt ttctgggctg
     3481 gctatagctg gcaggacaac agcgacggga gcggtgccgt gcgacccgac cgtgtcacgc
     3541 tgaaactgga taaagccagc tatcgccctg gcgataccat taagttgcat attgccgcgc
     3601 caacggcggg taaaggttat gcgatggtcg agtccagtga agggccgctg tggtggcaag
     3661 agattgatgt tccggctcaa gggctggatc tgacgattcc ggtcgataaa acctggaatc
     3721 gtcatgatct gtatttaagt acgctggtgg tacgtcctgg cgataaatct cgctccgcga
     3781 cgccaaaacg cgcggttggg gtgttgcatc tgccgcttgg tgatgaaaac cgtcgcctcg
     3841 atctggcgct ggaaacccct accaaaatgc gccccaatca gccattaacc gtgaaaatta
     3901 aagccagcaa taaaaatggt gagatgccaa aacaggtgaa tgtgctggtg tctgccgttg
     3961 atagcggtgt gctgaatatt actgattacg tcacgccaga tccgtggcag gcgttctttg
     4021 gtcagaaacg ctatggcgca gacatttacg atatttacgg tcaggtcatt gaaggtcagg
     4081 ggcgtctggc agctctgcgt tttggtggtg atggtgatga gctgaaacgt ggtggtaaac
     4141 cgccggtcaa tcacgtcaat attgtcgcgc agcaggcgct gccggtaacg ctcaacgaac
     4201 agggcgaagg ctcggttaca ctgccaattg gcgattttaa cggtgaattg cgcgtcatgg
     4261 cgcaagcctg gacggcagat gacttcggaa gcaacgaaag caaagtgatt gttgctgcac
     4321 cggtgattgc tgaactgaac atgccacgct ttatggcgag tggcgatacc tcgcgcctga
     4381 cgctggatat cactaatctt accgataaac cgcaaaaact taatgttgcc ctgaccgcca
     4441 gtggtttgct tgaactggtc agcgattcac ccgcagccgt tgaattagcg ccaggggtgc
     4501 gtactacgct gtttatcccg gtgcgtgcat tgccgggtta tggcgatgga gacattcagg
     4561 ccaccattag cgggttagcg ttacccgggg aaaccgttgc cgatcagcat aagcagtgga
     4621 aaatcggcgt ccgtccggca ttcccggcac aaacggtcaa ttacggtacg gcgttacagc
     4681 ctggtgaaac ctgggcgctc ccggctgatg gattgcaaaa cttctcgcct gttacgctgg
     4741 aagggcaatt gttgttgagc ggcaaaccac cgctgaacat cgcacgttat atcaaagagt
     4801 taaaagcgta tccgtacggc tgtctcgagc aaaccaccag cggcctgttc ccgtcacttt
     4861 ataccaatgc agcccaactg caggcgttgg gcatcaaagg cgacagtgat gagaaacgcc
     4921 gtgcatcggt cgatatcggc atttcccgtt tgctgcaaat gcaacgtgat aacggcggct
     4981 ttacgctgtg ggataaaaac ggtgacgaag agtactggct gacggcttac gtgatggatt
     5041 tcctggtccg cgcaggcgaa cagggttaca gcgtaccgac agacgccatt aaccggggta
     5101 atgagcgtct gctgcgctat ttacaagatc cgggcatgat gtcgatcccg tacgctgata
     5161 atctcaaagc cagtaaattc gccgtacagt cttacgctgc gctggtgttg gcccgtcagc
     5221 aaaaagcacc actgggtgcg ctgcgtgaaa tctgggagca tcgtgcagat gccgcttctg
     5281 gtttaccgct gctgcaactt ggcgttgcgc tgaaaaccat gggtgatgca atgcgtggtg
     5341 aagaagcgat tgtgctggcg ctgaaaacgc cgcgtaatag tgatgagcgg atatggctgg
     5401 gtgattacgg tagtccactg cgcgacagcg cgttgatgct ctcattgctg gaagagaaca
     5461 aactgctacc cgatgagcag tactccctgt tgaacacact ttcgcagcag gcgttcggtg
     5521 aacgctggct atcgacgcag gaaagtaacg cgttgttcct ggctgcccgt actcttcagg
     5581 atttaccggg caaatggcag gcgcaaacca ctttctctgc tgagccgctg acaggcgaga
     5641 aagcgcaaac cagcaatctg aatagcgatc aacttgccac cttgcaggtg accaacagtg
     5701 gcgatcagcc gttatggctg cgtgtggatg ccagcggtta tccgcaatcc gcacctttat
     5761 cggcgagcaa tgtgctgcaa attgagcgtc atattctggg tactgacggt aagagcaaat
     5821 cgctggactc gttacgtagc ggcgatctgg tgctggtgtg gttgcaggtt aaagccagta
     5881 acagtgtacc ggatgcgtta gtcgtggatc tgttgcctgc ggggctggaa ctggaaaacc
     5941 agaatctggc gaacggcagc gccagcctgg agcaaagtgg tggtgaagtg cagaacttgc
     6001 tgaaccagat gcagcaggcg agcattaagc acatagagtt ccgtgacgat cgctttgtgg
     6061 cggcggttgc cgttgatgaa tatcaaccgg taacgctggt gtatctggcg cgggcggtga
     6121 cgccgggaac gtatcaggtg ccgcagccta tggtggaatc aatgtatgtt cctcaatggc
     6181 gggcgactgg tgcggctgac gatctgttga tagtcagacc gtaaatgcct cgcctgttaa
     6241 ccaaacgcgg ctgctggata atgctggcag ccgcgccttt tatcatcatt ctggcagcat
     6301 gggcagcaga taaactctgg cctctgccat tgcaagaagt gaatcctgca cgcgtggtgg
     6361 tggcgcagga tggtacgcca ctctggcgtt tcgccgatgc tgacggtatc tggcgttatc
     6421 cggtaacaat cgaagatgtt tctccacgtt accttgaagc gctgatcaat tatgaagatc
     6481 gctggttctg gatgcatccg ggggtgaatc cgttctcggt ggcgcgtgca gcctggcaag
     6541 atctcacgtc gggtcgggtc atttccggcg gcagcacgct caccatgcag gtggcgcgcc
     6601 tgcttgatcc tcaccccaaa acgtttggcg gcaaaattcg ccagctctgg cgcgcgttgc
     6661 aactggaatg gcatctgtct aaacgtgaaa ttctgacctt gtatcttaac cgtgccccgt
     6721 ttggcggtac gttgcagggg attggtgcgg caagttgggc ttacctcggg aaatcgcctg
     6781 cgaatttaag ctattccgag gcggcaatgc tggcggtttt gccgcaagcg cccagccgtc
     6841 ttcgcccgga tcgttggccg gagcgtgccg aagccgcgcg taataaagtg ctcgaacgga
     6901 tggccgtgca aggtgtgtgg tcccgtgagc gggtaaaaga gtcaagggaa gaacccatct
     6961 ggctggcacc ccgacaaatg ccgcaactgg cactgctgtt ttcgcgcatg atgctcggta
     7021 aaagcaaaag cgacaaaatc gttactacgc tggatgccgg tcttcaacga cgtctggaag
     7081 aactggcgca aaactggaaa ggacgattgc caccgcgcag ctcactggcg atgatcgtgg
     7141 ttgatcatac cgatatgcgt gttcgcggct gggtgggatc agttgatctc aacgatgatt
     7201 cacgctttgg tcatgttgat atggtcaatg cgatccgatc gccaggatcg gtgctcaaac
     7261 cgtttgttta tggtctggcg ctggatgaag gcttgatcca cccggcatcg ctgttgcaag
     7321 acgtccccag acgcaccggt gattaccgac ctggcaactt tgacagcggt tttcatggcc
     7381 cgatcagcat gagtgaggcg ctggtgcgat cgctgaactt acctgctgtg caggtgctgg
     7441 aagcctatgg accgaaacgg tttgcggcaa agttacgcaa tgttggattg ccgttatatt
     7501 tgcccaacgg tgctgcaccg aatctttcgc tcattctcgg cggcgctggc gcaaaactgg
     7561 aagatatggc ggcagcgtat accgcgtttg ctcgccacgg caaggcaggc aaattgcgct
     7621 tacagcctga cgatccgctg cttgaacggc ctttaatgtc gtcgggggct gcgtggatca
     7681 ttcgccggat tatggctgat gaagcgcaac ccttgccgga tggtgccttg ccgcgcgtcg
     7741 ccccactggc atggaaaacg ggcaccagct atggctatcg tgatgcctgg gcgattgggg
     7801 ttaacgctcg ctatgtcatt gggatctgga ctggcagacc ggacggcacg cccgttgttg
     7861 gtcagtttgg ctttgccagt gccataccgc tgttgaatca ggttaataat atcttactgt
     7921 cgcgcagtgt gaatttgccg gaagacccac gtcctgactc tgtaagtcgt ggcgttatct
     7981 gctggccggg cggacagtct ttggcggaag gtgacggtaa ctgtcgccgc cgcctggcaa
     8041 cctggctgct ggacgggagc cagccgccaa ctctattact gccggagcag gaaggcatta
     8101 atggcattcg tttccccatc tggctggatg aaaacggcaa acgtgttgcc gctgattgcc
     8161 ctcaggcgcg acaagaaatg ataaatgtct ggccgctacc gctggaaccc tggctgcccg
     8221 catcagaacg tcgcgctgtg cgcttgccac cggcctcgac aatttgcccg ccatacggtc
     8281 acgatgcaca actcccgctg cagctaacag gtgtgcgcga tggcgcgatt attaaacgtt
     8341 taccgggcgc agcggaagcg actttgccgt tgcaatcaag tggaggggca ggtgaacgct
     8401 ggtggtttct gaacggcgaa ccgttaactg aacgtgggcg caacgtgacc ctgcatttga
     8461 cggataaagg cgattatcaa ttgctggtga tggatgaggt ggggcaaata gcgacagtga
     8521 aatttgtcat gcaatagtct gttttgtccc tgattgttgc ttaaactggg tttattttaa
     8581 aaaaatgtta ccttcatcaa tagtcaacgg ctctgttgct cattataatc cgcgccatct
     8641 cgtacgctgg tacagacaac aacagaacaa tttacagagg taaaaatggc tattgaacgt
     8701 actttttcca tcatcaaacc gaacgcggta gcaaaaaacg tgattggtag tatctttgcg
     8761 cgctttgaag ctgcagggtt caaaattgtc ggcaccaaaa tgctgcacct gaccgttgaa
     8821 caggctcgtg gcttttatgc tgaacacgat ggaaaaccgt tctttgatgg tctggttgaa
     8881 ttcatgactt ctggcccgat cgttgtttcc gtgctggaag gtgaaaatgc tgttcagcgt
     8941 caccgcgatc tgctgggcgc gaccaatccg gcaaacgcac tggccggtac tctgcgtgct
     9001 gattacgctg acagcctgac cgaaaatggt acccacggtt ctgattccgt cgaatctgcc
     9061 gctcgcgaaa tcgcttattt cttcggcgaa ggcgaagtgt gcccgcgcac ccgttaataa
     9121 tttcgtaaat gccgcgtgca aacgtggcat ccgtgcgcca gaatttgtac aatgcagcgc
     9181 ccccggacga gcagccgctc accggggcgt ttcttttttc aaccctccag gggccataac
     9241 gtgtaataac gaggccggaa tagcatatgt ctgaacaatt agtcacacct gaaaacgtca
     9301 ccactaaaga tggaaaaatc aacctgctgg atctcaaccg tcagcagatg cgggagtttt
     9361 ttaaagattt aggtgaaaaa cccttccgcg ccgatcaggt gatgaagtgg atgtatcact
     9421 attgctgcga caactttgat gagatgaccg acatcaacaa agtgttgcgc ggcaaactga
     9481 aagaggtggc ggagatccgc gcaccggaag tggttgaaga acagcgttca tctgacggca
     9541 ccattaaatg ggcaattgcc gttggcgatc agcgcgtcga aacggtgtat atcccggaag
     9601 acgaccgtgc cacgctctgc gtctcttcgc aggtggggtg tgcgctggag tgtaaattct
     9661 gttccaccgc ccagcagggc tttaaccgca acctgcgggt atcggaaatt atcggccagg
     9721 tgtggcgtgc ggcgaaaatt gtcggcgcag caaaagtcac cggtcagcgc ccaatcacta
     9781 acgtcgtgat gatgggtatg ggcgagccgc tgctcaacct gaacaacgtc gttccggcga
     9841 tggaaatcat gcttgatgat ttcggttttg gcctgtcaaa acgtcgcgtc acgctttcca
     9901 cttccggtgt ggttccggcg ctggataaac tgggcgatat gatcgacgtt gcgctggcta
     9961 tctccctgca cgcgccgaac gacgaaattc gtgacgaaat tgtgccgatc aacaaaaagt
    10021 acaatatcga aacgttcctt gcggcggtgc gccgttatct ggagaaatcc aacgccaatc
    10081 agggccgagt cactattgaa tacgtgatgc ttgaccacgt taacgacggc actgaacacg
    10141 cgcaccaact ggcggaactg ctgaaagata cgccgtgtaa gatcaacctg atcccgtgga
    10201 acccgttccc gggcgcgccg tatggacgca gttcgaacag ccgtatcgac cgtttctcaa
    10261 aagtgctgat gagctacggt ttcaccacta ttgtgcgtaa aacgcgtggt gatgacattg
    10321 atgctgcctg tggtcagttg gcgggcgatg ttatcgaccg tacgaaacgt accctgcgta
    10381 aacgaatgca gggtgaagcc atcgacatta aagcggtctg atagccatta cgccacggtt
    10441 actttatgta ccgtggcgta atcttttatg aatgaagtat taagttgcaa ttgtctggtc
    10501 aatattaacg gtgcgttttt gctgacttta aggcagtatg taacgatgca acagtaagtt
    10561 agccttagcg aatgtgggct ttgtcacgag cacacagacg gtcttatact gtatgataac
    10621 ggttaactta acggatgttt cgcggtgtgg gtgggcatga tggttcaccg gcatctcaat
    10681 tctcatttaa acgtacctgc agcgaatgaa tactgaagcc acgcacgacc aaaatgaagc
    10741 acttactacc ggcgctcgcc tgcgtaatgc tcgcgaacaa ctaggactta gtcaacaggc
    10801 cgttgccgag cgactttgcc tgaaggtttc cacggtacgc gacattgaag aagataaggc
    10861 acccgccgat cttgcttcaa cattcctgcg cggatatatc cgctcttatg cgcgtctggt
    10921 acatattcca gaagaagaac tgctgccagg gctggaaaag caggctccac tacgggctgc
    10981 aaaagttgcg ccgatgcaga gtttttccct cggtaaacgc cgcaaaaaac gtgacggctg
    11041 gctgatgacc ttcacctggc tggtattgtt tgtggttatt ggcctgagcg gtgcctggtg
    11101 gtggcaagac cacaaagctc agcaggaaga gatcaccact atggccgatc aatcttctgc
    11161 ggaactgaat aataatcaaa gccagagtgt accgttagat acgtcgacaa ccacagatca
    11221 ggctatggcg accacgccga catcacctgt ggataccacc gcaaccaaca cgcaaacacc
    11281 tgccgtaact gcgccagcac cagctgttga tccgcaacag aatgcggttg ttccaccttc
    11341 gcaggcaaat gttgataccg ctgcgactcc ggcaccagcg gcaacaacaa caccagatgg
    11401 cgctgcgccg ttgccaaccg atcaggctgg cgtgaccacg ccggcggttg acccgaatgc
    11461 gctggtgatg aactttactg ccgattgctg gctggaagtc accgatgcta ccggtaaaaa
    11521 actgtttagc ggtatgcagc gtaaagacgg taatttgaac ttaaccggcc aggcaccgta
    11581 caaactgaaa attggtgcgc cagccgcagt acagatccag tatcaaggga aacctgtcga
    11641 tctgagtcgt tttatcagaa ctaaccaggt tgcgcgtctg accctcaatg ccgaacaatc
    11701 accggcgcag taacagacgg gtaacgcggg agatttttca tgcataacca ggctccaatt
    11761 caacgtagaa aatcaacacg tatttacgtt gggaatgtgc cgattggcga tggtgctccc
    11821 atcgccgtac agtccatgac caatacgcgc acgacagacg tcgaagcaac ggtcaatcaa
    11881 atcaaggcgc tggaacgtgt tggcgctgat atcgtccgtg tctccgtacc gacgatggac
    11941 gcggcagaag cgttcaaact catcaaacag caggttaacg tgccgctggt ggctgacatc
    12001 cacttcgact atcgcattgc gctgaaagta gcggaatacg gcgtcgattg tctgcgtatt
    12061 aaccctggca atatcggtaa tgaagagcgt attcgcatgg tggttgactg tgcgcgcgat
    12121 aaaaacattc cgatccgtat tggcgttaat gccggatcgt tggaaaaaga tctgcaagaa
    12181 aagtatggcg aaccgacgcc gcaggcgttg ctggaatctg ccatgcgtca tgttgatcat
    12241 ctcgatcgcc tgaacttcga tcagttcaaa gtcagcgtga aagcgtctga cgtcttcctc
    12301 gctgttgagt cttatcgttt gctggcaaaa caaatcgatc agccgctgca tctggggatc
    12361 accgaagccg gtggtgcgcg tagcggggca gtaaaatccg ctattggttt aggtctgctg
    12421 ctgtctgaag gcatcggcga cacgctgcgc gtatcgctgg cggccgatcc ggtcgaagag
    12481 atcaaagtcg gtttcgatat tttgaaatcg ctgcgtatcc gttcgcgcgg gatcaacttc
    12541 atcgcctgcc cgacatgttc gcgtcaggaa tttgatgtta tcggtacggt taacgcgctg
    12601 gagcaacgcc tggaagatat catcactccg atggacgttt cgattatcgg ctgcgtggtg
    12661 aatggcccag gtgaggcgct ggtttctaca ctcggcgtca ctggcggcaa caagaaaagc
    12721 ggcctctatg aagatggcgt gcgcaaagac cgtctggaca acaacgatat gatcgatcag
    12781 ctggaagcgc gcattcgtgc gaaagccagt cagctggacg aagcgcgtcg aattgacgtt
    12841 cagcaggttg aaaaataata acgtgatggg aagcgcctcg cttcccgtgt atgattgaac
    12901 ccgcatggct cccgaaacat tgagggaagc gttgagggtt catttttata ttcagaaaga
    12961 gaataaacgt ggcaaaaaac attcaagcca ttcgcggcat gaacgattac ctgcctggcg
    13021 aaacggccat ctggcagcgc attgaaggca cactgaaaaa cgtgctcggc agctacggtt
    13081 acagtgaaat ccgcttgccg attgtagagc agaccccgct attcaaacgt gcgattggtg
    13141 aagtcaccga cgtggttgaa aaagagatgt acacctttga ggatcgcaat ggcgacagcc
    13201 tgactctgcg ccctgaaggg acggcgggct gtgtacgagc cggcatcgag catggtcttc
    13261 tgtacaatca ggaacagcgt ctgtggtata tcgggcctat gttccgtcac gagcgtccgc
    13321 agaaagggcg ttatcgtcag ttccatcagt tgggctgcga agttttcggt ctgcaaggtc
    13381 cggatatcga cgccgaactg attatgctca ctgcccgctg gtggcgcacg ctgggtattt
    13441 ccgagcacgt aactcttgag ctgaactcta tcggttcgct ggaagctcgt gcgaattacc
    13501 gcgatgcgct ggtggcattc cttgagcagc ataaagaaaa gctggacgaa gactgcaaac
    13561 gccgcatgta cactaacccg ttgcgcgtac tcgattcaaa aaatccggaa gtgcaggtgc
    13621 ttctcaacga cgctccggca ttaggcgatt atctggacga ggaatctcgt gagcactttg
    13681 ccggtctgtg caaactgctt gagagcgcgg gtatcgctta caccgtaaac cagcgtctgg
    13741 tgcgcggtct ggattactat aaccgtaccg ttttcgagtg ggtgactaac agcctcggtt
    13801 cccagggcac cgtgtgtgca ggtggtcgtt atgacggtct ggtggaacaa ctgggtggtc
    13861 gtgcaacacc ggcagtcggt tttgcgatgg gcctcgaacg tcttgtattg ttagttcagg
    13921 ccgttaatcc ggaatttaaa gccgatcctg ttgtcgatat atacctggtg gcttcaggtg
    13981 ctgacacaca atctgcggct atggcattag cagagcgtct gcgtgatgaa ttaccgggcg
    14041 tgaaactgat gaccaaccac ggcggcggca actttaagaa acagtttgcc cgtgctgata
    14101 aatggggtgc ccgcgttgct gtggtgctgg gtgagtctga agtggctaac ggcacagcag
    14161 tagtgaagga tttgcgctct ggtgagcaaa ctgcagttgc gcaggatagc gtagccgcgc
    14221 atttgcgcac gttactgggt taaggaagga gaaggacagc gtggaaattt acgagaacga
    14281 aaacgaccag gttgaagcgg ttaaacgctt ttttgctgaa aatggcaaag cactggctgt
    14341 tggggtgatt ttgggcgttg gcgcactgat tggctggcgc tactggaata gccatcaggt
    14401 tgattctgca cgctccgctt ctcttgccta tcaaaatgcg gttaccgcag tgagcgaagg
    14461 caaaccggat agcatcccag cggcggaaaa atttgctgct gaaaataaaa atacttatgg
    14521 tgcgctggct tctttggaac ttgcgcagca atttgttgac aaaaatgaac tagagaaagc
    14581 tgccgcccag ttacaacagg ggctggcaga cacgagcgat gaaaatctca aagccgtgat
    14641 aaatctgcgt cttgctcgcg ttcaggtaca gctcaagcag gctgatgccg cgctaaaaac
    14701 ccttgatgct atcaaaggtg aagggtgggc tgccattgtt gccgacctgc gtggtgaagc
    14761 attgctgagc aaaggtgata agcaaggtgc gcgtagtgca tgggaagcag gcgtgaaaag
    14821 cgatgttacc ccggcactga gcgaaatgat gcagatgaaa attaataatt tgtccatctg
    14881 agagggaccc gatgcaattg cgtaaattac tgctgccagg actgctttcc gttacccttt
    14941 taagcggctg ttcgctgttt aacagcgaag aagatgtggt aaagatgtcc ccattgccaa
    15001 ccgttgaaaa ccagtttacg ccgactactg cgtggagcac ttccgttggt agcggcattg
    15061 gcaacttcta ttccaatctt catccggcac tggcggacaa cgttgtctat gcagcggacc
    15121 gcgctggttt agtaaaagcg ctgaatgcgg atgacggcaa agaaatctgg tctgtcaacc
    15181 tggccgagaa agatggctgg ttctctaaag atcctgcatt actttctggc ggcgtgaccg
    15241 tgtctggtgg gcatgtctac attggcagcg aaaaggcgca ggtttacgcg ctgaatacca
    15301 gcgatggtac tgtggcatgg caaactaaag tcgcgggtga agcactttcg cgcccggtgg
    15361 tcagcgacgg tctggtgtta atccacacca gtaacggtca gttacaagcg ctgaacgaag
    15421 ctgacggcgc tgtcaaatgg acagttaacc tcgatatgcc gtcgctctct ttgcgtggcg
    15481 agtctgcgcc ggcaacggct tttggtgcgg ccgtcgtggg tggcgataat ggtcgcgtca
    15541 gcgcagtgct gatggaacag ggccagatga tttggcagca gcgtatttcc caggcgaccg
    15601 gttctaccga aattgaccgt ctgagcgatg ttgacacgac tcccgtcgtt gttaacggcg
    15661 ttgttttcgc gctggcctat aatggtaacc tgacggcgct tgatctgcgc agcggtcaga
    15721 ttatgtggaa acgcgaactg ggttcggtga atgatttcat cgtcgacggc aaccgcatct
    15781 atctggtcga tcaaaatgac cgggtgatgg cgttgaccat tgatggcggc gttacgctgt
    15841 ggacacaaag cgatctgctg catcgcctgc tgacttctcc ggtgctgtat aatggcaacc
    15901 tggtggtagg tgacagtgaa ggttatctgc actggattaa cgtcgaagat ggtcgtttcg
    15961 ttgcccagca aaaagttgat agttccggtt tccagactga accggttgct gctgacggca
    16021 aactgctgat ccaggcaaaa gacggaaccg tgtactctat tacacgttaa tcgtctctgt
    16081 cgttcacttt gaaaacggct cctggacagg ggccgttttc ctgtttttaa caacgacacg
    16141 aatatagcgt gcgttgtctg atgatttata aaaatgaggc tttaaacatg gtacctgtgg
    16201 tcgcgcttgt cgggcgccct aacgtaggaa aatccacgtt atttaaccgt ctaactcgca
    16261 cccgagatgc gctggttgcg gatttcccgg gtctgactcg tgaccgtaag tacggtcgtg
    16321 cggaaattga aggccgcgag tttatctgca ttgataccgg cgggattgat ggcaccgaag
    16381 acggtgtaga gacacgtatg gcggaacagt cgctgctggc gattgaagaa gcggacgttg
    16441 tactgtttat ggtggatgcg cgcgcgggcc tgatgccggc ggatgaagcg attgctaaac
    16501 atctgcgctc ccgtgaaaaa ccgaccttcc tggtggcaaa caaaactgac ggtctggatc
    16561 ccgatcaggc agtggttgat ttctacgcgc ttggtttagg tgaaatctac ccgatcgccg
    16621 cgtctcacgg tcgtggcgta ttaagtctgc tggagcacgt gctgctgccg tggatggaag
    16681 atctcgcacc gcaagaagaa gtcgacgaag acgccgaata ctgggcgcaa tttgaagcgg
    16741 aagagaacgg cgaagaagaa gaggcagacg acttcgaccc gcaaagtctg ccgatcaaac
    16801 tggcgattgt gggtcgtccg aacgtaggta agtctacact cactaaccgt attcttggtg
    16861 aagagcgcgt tgttgtttac gacatgcctg gcacgacgcg tgacagtatc tacattccaa
    16921 tggaacgcga cggacgtgag tatgtgctca ttgacaccgc tggtgtacgt aaacgcggca
    16981 aaatcaccga tgcggtagag aaattctcgg taatcaaaac gttgcaggcc attgaagatg
    17041 ccaacgtggt gatgttggtg attgatgcgc gtgaaggtat ttccgatcag gatctctcct
    17101 tgctgggctt tattctcaat agtgggcgct cacttgtgat tgtagtgaac aagtgggacg
    17161 gcctgagcca ggaagtgaaa gagcaggtga aagagacgct ggacttccgt ctgggcttta
    17221 tcgattttgc tcgtgtgcac tttatctctg ccttgcacgg cagtggtgtt ggtaacttgt
    17281 ttgaatcagt acgtgaagcg tatgacagct ccacccgtcg tgtggggacc tctatgctga
    17341 cgcgcatcat gacgatggct gttgaagatc accaaccacc gctggtacgc ggtcgtcgtg
    17401 tgaagctgaa atacgcccac gccgggggtt ataacccgcc gattgtggtg attcacggca
    17461 atcaggtgaa agacctgcct gattcctaca agcgttacct gatgaactac ttccgcaaat
    17521 cgctggacgt aatgggatcg ccgattcgta ttcagttcaa agaaggggaa aacccgtatg
    17581 cgaacaagcg taacaccctg acgccaaccc agatgcgtaa acgtaagcgt ctgatgaagc
    17641 atatcaagaa aagtaagtaa tcacttatct ggtttccctt tccacacgga gagggaagcc
    17701 gaataacacg cgaggagtat ttatggcact caactgtcca acctgcaaaa cccctctcga
    17761 acgcaatggc gagactgctc actgtgcaac ttgcgacaaa gactttaccg tccaggcact
    17821 ttgccctgag tgtcatcagg cgttacaagt gttaaaagct tgtggtgctg tggattattt
    17881 ctgccaacat gggcacggtt taatatctaa aaaacgcgtt gaatt
//
LOCUS       DAZKGF010000099        17866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZKGF010000099 DAZKGF010000000
VERSION     DAZKGF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.099"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(45..698)
                     /gene="fdnI"
                     /locus_tag="ACHOQV_003152"
     CDS             complement(45..698)
                     /gene="fdnI"
                     /locus_tag="ACHOQV_003152"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFP9631486.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(691..1575)
                     /gene="fdnH"
                     /locus_tag="ACHOQV_003153"
     CDS             complement(691..1575)
                     /gene="fdnH"
                     /locus_tag="ACHOQV_003153"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFP9631487.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVNLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEEHHE"
     gene            complement(1588..4635)
                     /gene="fdnG"
                     /locus_tag="ACHOQV_003154"
     CDS             complement(1588..4635)
                     /gene="fdnG"
                     /locus_tag="ACHOQV_003154"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4048..4050),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP9631488.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4867..5748
                     /gene="yddG"
                     /locus_tag="ACHOQV_003155"
     CDS             4867..5748
                     /gene="yddG"
                     /locus_tag="ACHOQV_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFP9631489.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LSFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            6021..7409
                     /gene="narU"
                     /locus_tag="ACHOQV_003156"
     CDS             6021..7409
                     /gene="narU"
                     /locus_tag="ACHOQV_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFP9631490.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            7504..11244
                     /gene="narZ"
                     /locus_tag="ACHOQV_003157"
     CDS             7504..11244
                     /gene="narZ"
                     /locus_tag="ACHOQV_003157"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFP9631491.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELLLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVF
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGPTEGIQPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDMVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLAQHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            11241..12785
                     /gene="narH"
                     /locus_tag="ACHOQV_003158"
     CDS             11241..12785
                     /gene="narH"
                     /locus_tag="ACHOQV_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP9631492.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLNVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            12785..13480
                     /gene="narW"
                     /locus_tag="ACHOQV_003159"
     CDS             12785..13480
                     /gene="narW"
                     /locus_tag="ACHOQV_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP9631493.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            13477..14157
                     /gene="narI"
                     /locus_tag="ACHOQV_003160"
     CDS             13477..14157
                     /gene="narI"
                     /locus_tag="ACHOQV_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP9631494.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            14237..15130
                     /locus_tag="ACHOQV_003161"
     CDS             14237..15130
                     /locus_tag="ACHOQV_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFP9631495.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFESPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPDKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGVAVILFHAEWAIEL"
     gene            complement(15226..16071)
                     /gene="nhoA"
                     /locus_tag="ACHOQV_003162"
     CDS             complement(15226..16071)
                     /gene="nhoA"
                     /locus_tag="ACHOQV_003162"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFP9631496.1"
                     /translation="MTPILNHYFARINWSGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            16246..16815
                     /locus_tag="ACHOQV_003163"
     CDS             16246..16815
                     /locus_tag="ACHOQV_003163"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP9631497.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFYCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(16969..17349)
                     /locus_tag="ACHOQV_003164"
     CDS             complement(16969..17349)
                     /locus_tag="ACHOQV_003164"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9631498.1"
                     /translation="MIIRPVKPSDVEPLLDMLLASGQFNQDGLHHVQKTLTHYFSGQS
                     ADLWFSAEHLGLAGIAYCAPEVMTNDVWNLLMHWISPSHQRQGVGHLLINEIEKELTI
                     KQARLLLVETSSYVDFSAARAFYT"
     gene            complement(17629..>17866)
                     /locus_tag="ACHOQV_003165"
     CDS             complement(17629..>17866)
                     /locus_tag="ACHOQV_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631499.1"
                     /translation="LDKILAFSDKNKHEYFLYIKAYWLSLYFCEISQKVRERDKLSFY
                     YDEFSVRFPNVVLYPTYFQLHGLASEKSLLIRDA"
BASE COUNT         4243 a         4682 c         4527 g         4414 t
ORIGIN      
        1 ggcgcttctt aaagcgccat tttcagctta aagttcaaaa gagattatat cccttcttca
       61 ctctcttttt tcgcttccgc cttctcgatt tcacgatacc agcgcggatg gtgtttcttc
      121 gcccagcgac ggctcacctt cccttcgatc atccctttaa tcgatccttt cacccagaat
      181 gccatataca tatggatcag gatggcgtgg atcaggatga tacccgcagc cgcgtgaatc
      241 agcaggctgt agcgaacaac ctgcatcggg aagtactgcg caaagtacgg acgccagata
      301 atcaccccgg tcaccagcag cacgaaaatc atgctcatga tcgaccagaa catcatcttc
      361 tgcccggcgt tgtacttacc aacatccgcc actttgtgct cattgccttt taatacttcg
      421 acaatgttca acagccacgg aatatccttc ttatccggga tattgtgatg tacaaaacgc
      481 acaaacataa acatcagcgc gacgaaaatc gcaatgccga agaacgggtg caaaatgcgt
      541 cccatctgcg gcgtaccgaa ggtttgcgtc agccattgca gcgtcgggaa gaagaacgaa
      601 atcccggaca gcgccaccag gaagaagcaa atcaccacgg tccagtgaca ggcgcgatca
      661 ataaacttgg tgcgcacaat cattttcgac ttactcatga tgctcctcct cgtcatcgtc
      721 cacttcctta ttcgggccaa taccgatgta gtggaaaatc aacccggcaa aagtggcgat
      781 aaagccagcc gctgccagcg gtttcaacgc gcctttccac aagttcaccg atgtgtcgat
      841 cttcggatct ttcggcagac cgtgatacag ctccggctga tcggcgtgat gcaacacgta
      901 catgacgtgc gtaccaccga ccccttccgg gttgtagacg ccagcatgtt cgtaaccacg
      961 cgctttcagt ttcgctacgc gctgttccgc cagctccagc atctccttct tggtgccgaa
     1021 gtggatagcc ccggtcggac aggttttcac acaagccggt tcctggccga cgctgacgcg
     1081 atcgacgcag agcgtgcatt tatatacccg gttatcctct ttgttgaggc gcggaatatt
     1141 aaacggacac ccggcaatgc agtaaccaca gccgatgcag ttttccgact ggaaatcaac
     1201 aatcccgtta gcgtactgaa tgattgcacc agcagacggg cacgccttca ggcagcccgg
     1261 atcttcacag tgcatacagc catctttacg gatcagccac tccagcttgc cgttctgttc
     1321 ggtttcgcta aagcgcatca ccgtccagga cttggcgctc agatcggcgg ggttatcgta
     1381 aaccccgacg cagtgcccca cttcatcacg gatgtcgttc cactccgaac acgccacctg
     1441 acaggcttta cagccgatac aggtggaaac gtcgataagt ttcgcgactt ctgctttgta
     1501 atcacgcacc tgagaaggcg gcgtgatgga gttagttgcg gaccttttga taatgtcctg
     1561 cgtttccata gccatctgtt cgccccctta cgccttctcg atgtttacta agaacgcttt
     1621 atattccggc gtttgcgagt ttgcatcacc gacattcggc gtcagagtgt tagcgatata
     1681 acctttacgc gcgacaccct caaagcccca gtggattgga atacccaccg tttcaacctg
     1741 ctgaccgttt acattcagcg gtttcagacg gcgcgttacc acagccaccg cgcggataaa
     1801 gccacgcttg ctggagacag tgacacgatc gccattggca atgcctttcg ccgccgccag
     1861 cgtttcgctg atttccacaa actgttccgg ctgagcaatt gcgttgagca atgcgtgctt
     1921 ggtccaggtg tggaagtgct ccgtcagacg ataggtcgta cccacatacg ggaactgctc
     1981 ttttttaccc atccgcagtg cgtcttgttc atacagacga acaaccgggt tagacaccac
     2041 gttcggatgc aacgggttag tgcccagcgg cgtttcaatt ggctcatagt gttccgggaa
     2101 cggaccttcc gccattttgt tgatggcaaa caggcgtccc atcccttccg gctgcatgat
     2161 aaacggcccg gttggcgtac ccggtgcggc attgccgaag tccggaatat cgttacccgt
     2221 ccacttgcta ccgttccact ggatcagcat ccgtttcgga tcccacggtt taccgttgat
     2281 atccgccgaa gcacggttgt agagtacgcg acggttgagc ggccacgccc aggcccatcc
     2341 cagcgtattc cccagaccgg acgggtcgga gttatcgcgg ttagccatct ggttgccctg
     2401 ctcggtccag ctaccggtgt agatccagca agaagatgcg gttgtaccgt catcacgcag
     2461 atgcgcaaag ctactcagca actgaccttt cttcgcaatc agcacgccat tagcgtcata
     2521 gagatcttcc agcgcatagc cgttgttctc tttcgccact tcgtcagact gcggttcgtg
     2581 cggctgcttg tagttccagc tcatcttcat cagcggttct acgcctttac caccctcggc
     2641 ctggtacagc tcgcgcagat gatggtagat acccgccaga atttcgccgt cgttacgtgc
     2701 ttcgcccggc gcgtcctgac ctttccagtg ccactgcaac cagcgaccgg agttagcgat
     2761 agaaccatct tcttcagcaa agcaggtcga aggcagacgg aatacttcag tctgaataga
     2821 tgccggatcg acatcgttcg actcgccgtg gttctgccag aaggtagagg tttcagtcac
     2881 cagcggatcg ataaccacca tgtacttcag tttgctcagg cagctcacca ctttgttttt
     2941 gtccgggaag gacgcaaccg ggttaaagcc ctggcagaaa taaccggtga ctttaccttc
     3001 atccatcatg ttgaaatact tgatgacgtc gtaggtctgg tcccacttcg gcagccagtc
     3061 gtagccccag ttgttctctt tctgcgcggc atcgccgtag aaagatttca tcaggctaac
     3121 gaaaaacttc ggatagttgc tccagtagtt cacctgatca gccagcgtcg ctttcggcgt
     3181 gttcgcttcc agatacgact gcaaatcaac ctgtttttct gacggcagcg tcagataacc
     3241 tggcaggctg gtagagagca ggcctaagtc agtcaggccc tgaatgttgg agtgaccacg
     3301 caatgcgttc acgccgccac cggccatacc catgttaccg agcagcaact ggatcatcgc
     3361 catcgtacgg atgttctgcg cacccacggt gtgctgcgtc cagcccagcg cgtacaggaa
     3421 ggtggttgtg cgatccggtg cgctggtgga ggccagcact tcacacactt tcaggaagtc
     3481 ggcttttggc gtaccacaga tgttttcaac aacgtccggc gtgtagcggg aaacgtgcgc
     3541 tttcagcagg ttccacacac agcgcggatg agtcagtgtt tcatcgcgtt tcgcatagcc
     3601 gttttcatcg aactgatagt tccaggacga tttatcgtat tgccgttttt cagcgtcgta
     3661 gccgctgaac agaccgtctt cgaaagcaaa atcatcacgc accagcaggc tggcattggt
     3721 gtaatgctta acgtattcgg cattgatttt gttgttttcg atcaggtagc gcaaaacgcc
     3781 agacaggaac gtaatgtccg taccggaacg aataggtgcg taaatatccg ctacagaagc
     3841 ggtacgcgta aaacggggat cgacaacgat caaggttgca tcgttgttgt ttttcgcttc
     3901 catcgcccag cggaaaccga cgggatgcgc ttcagcagcg ttaccgccca tcaccattac
     3961 gacgttagcg tttttgatat ccacccagtg gttggtcatc gcaccgcgac caaatgttgg
     4021 agcaagactt gctaccgttg gtccgtgtca gacgcgcgcc tggttgtcta ctgccagcat
     4081 cccgagggag cgggcaaatt tttgagtcag catcccggtt tcgttgctgg caccggaggc
     4141 acacagcata ccggtagaaa gccaacggtt taccgttacg ccctgctcgt tcttttcaat
     4201 aaagttagcg tcacggtcag ctttcatcag cttcgcaata cgggagaatg cttcttccca
     4261 gctaatgcgc tgccatttgt cagaacctgg cgcacgatat tccgggtagc gcagacggtt
     4321 ttcgctgttg acgtaatcca gcaaaccggc ccctttcggg cacagcgcac cacggcttac
     4381 cggatgatcc gggtcacctt caatgtgata aatcgcttct ctggcgtttt tcgcgccatc
     4441 acccaggcta tacatcaata gcccgcaacc tacggaacag tatgtgcagg tgttacggat
     4501 ctctttcgcg cgtaataatt tgtagtttcg cgcctgagcc agtgcttgct tcggggcaaa
     4561 gcccaatgcc gctaccgttg ttccagccat accgcccgcg cagattttaa aaaattgtct
     4621 gcgactgacg tccattgctt tcctcttttt tcaggggtat tactgcgcga ggaaactaac
     4681 agtaaaattc tgaatctttt tgcgcgaaat caaaaagaac acgtgtttgc cacaaaataa
     4741 tccgcctgcg caaattgacc tacctcaata gcggtagaaa aacgcaccac tgcctgacag
     4801 gccagttaaa aaaatgctat aaaattcagc ttaattttta acggcaagag agacaaaaca
     4861 gcgagcatga cacgacaaaa agcaacgctc atagggctaa tagcgatcgt cctgtggagc
     4921 acgatggtag gattgattcg cggtgtcagt gaggggctcg gcccggtcgg cggcgcagct
     4981 gctatctatt cattaagcgg gctgctgtta atcttcacgg ttggatttcc gcgtattcgg
     5041 cagatcccga aaggctattt actcgccggg agtctgttat tcgtcagcta tgaaatctgt
     5101 ctggcgcttt ccttagggta tgcggcgacc cgtcatcagg cgattgaagt ggggatggtg
     5161 aactatctat ggcccagcct gacaattctc tttgccattc tgtttaatgg tcagaaaacc
     5221 aactggctga ttgtaccagg attattatta gccctggtcg gcgtctgctg ggtgttaggt
     5281 ggtgacaatg ggttacatta tgatgaaatc atcaataata tcaccaccag cccattgagt
     5341 tatttcctgt cgtttattgg tgcgtttatc tgggcagcct actgcacagt aacgaataaa
     5401 tacgcacgcg gatttaacgg aattaccgtt tttgtcctgc taacgggagc aagtctgtgg
     5461 gtttactatt ttctcacgcc acaaccagaa atggtattta gcacgccagt catgattaaa
     5521 ctcatctctg cggcatttac cttaggattt gcttatgctg catggaacgt cggtatatta
     5581 catggcaacg tcaccattat ggcggtaggt tcgtatttta cgcccgtcct ttcctcagcg
     5641 cttgcagccg tgctgctcag cgccccgctg tcgttctcgt tctggcaagg cgcgctaatg
     5701 gtctgcggcg gttccctgct ctgctggctg gcgacacgtc gtggctaaaa taattgtcgg
     5761 gtctacggcc cggcagttaa tcagataaat tctgacaata ttaaaaaata aatttcaaat
     5821 accagaaagc cattttagtt acatttagta acacatatct gtctgtttaa tccacattct
     5881 gcccccgcag ttacgctttc ttatctattc ttctaatcag cacttcacct gattcatcct
     5941 taattctaat aactggctat tgcgccttac ctcctgtttt gtcttttttt cattaccaat
     6001 gtgtgcatgt gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac
     6061 gcgactggaa accagaaaat ccggccttct gggaaaataa aggaaagcat attgcacgaa
     6121 gaaacctctg gatatcagtc agttgcctac ttcttgcctt ctgtgtctgg atgctattta
     6181 gcgcagttac tgttaatctc aataaaatcg gttttaattt cactaccgat caactctttt
     6241 tattaaccgc attaccctcc gtttctggcg cattattgcg tgtcccctac tcctttatgg
     6301 tgcctatatt cggtggacgc cgatggacgg tttttagtac tgcaatcctg attattcctt
     6361 gcgtctggct cggaattgcc gtgcagaatc cgaatactcc ttttgggata tttatcgtta
     6421 tcgctttgct atgcggtttt gcaggtgcga actttgcttc gagcatgggc aatatcagtt
     6481 tcttctttcc aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact
     6541 taggtgtaag tgtgatgcag ctggttgcac cgctggtcat ttttgtcccc gtatttgcct
     6601 ttctcggcgt caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg
     6661 catggatttg ggtgccatta ctggcgattg ccacgatcgc cgcctggtca gggatgaatg
     6721 atatcgccag ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc
     6781 tctggctgct gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg
     6841 gttttgccat gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct
     6901 ttggcccatt tatcggtgcc atcgcgcgat cggttggtgg tgctatttcc gataaattcg
     6961 gcggcgtgcg ggtgacgttg atcaacttca tttttatggc gattttcagc gccctgctgt
     7021 tccttacctt accgggcaca ggctccggta atttcatcgc attttacgcc gtatttatgg
     7081 ggctgtttct gaccgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct
     7141 ttcgccagat aacaatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaaa
     7201 gagaagccat caccgaaacg gcagccgctc tgggctttat ctcagccatt ggcgcagtgg
     7261 gcggcttttt tattccgcag gcgtttggca tgtcgctcaa tatgaccggc tctccggtgg
     7321 gcgcgatgaa agtgttttta atcttctaca tcgtttgtgt gctactgacc tggctggttt
     7381 atggtcggcg gaagttcagc caaaaataag agatcgatgc ctgacacgtc ttatcaggct
     7441 acaaccgtgt cgcatccgac aaggcatcag atcccaattc attcctggag caggagttat
     7501 gtcatgagta aacttttgga tcgctttcgc tacttcaaac aaaagggcga aacctttgcc
     7561 gatggtcacg gacaggtgat gcatagcaac cgcgactggg aggacagcta tcgccagcgt
     7621 tggcagttcg acaaaatcgt gcgttccacc cacggtgtta actgtacagg ctcctgtagc
     7681 tggaaaatct acgttaaaaa tggtctggtg acctgggaaa tccaacagac cgactacccg
     7741 cgcactcgcc ctgacctgcc aaatcatgaa cctcgcggct gcccgcgtgg cgcaagttac
     7801 tcctggtatc tctacagcgc taaccgcctg aaataccctc tcattcgtaa acgactgatt
     7861 gaactgtggc gcgaagccct caagcaacac agcgatccgg tactggcgtg ggcatcgatt
     7921 atgaacgatc cgcaaaagtg cctgagctac aaacaagtac gtggacgcgg cggctttatc
     7981 cgctctaact ggcaggagct aaatcagctg attgctgccg ctaacgtctg gaccatcaaa
     8041 acctacggcc cggatcgcgt tgccggtttc tcgccgatcc cggcgatgtc gatggtttct
     8101 tacgccgccg gaacacgtta tctttcacta ttaggtggca cctgtttaag cttctacgac
     8161 tggtattgcg acctgccgcc cgcctcgccg atgacctggg gcgagcaaac cgacgtaccg
     8221 gaatctgccg actggtataa ctccagttac atcatcgcct gggggtctaa cgtaccgcag
     8281 acacgtacgc cggacgccca tttctttacc gaagtacgct acaaaggcac taaaaccatc
     8341 gccattaccc ctgactactc tgaagtggcc aaattgtgcg accagtggct ggcaccgaaa
     8401 caaggcactg atagtgccct ggcaatggca atgggccatg tcattttaaa agagtttcat
     8461 ctcgataatc ccagcgatta ttttattaac tactgccgcc gctacagcga catgccgatg
     8521 ctggtaatgc tggaacctcg cgacgacggc agctacgttc ccgggcgcat ggtccgcgca
     8581 tctgacctgg tggatggact gggcgaaagc aataatccgc agtggaaaac cgtggcagtt
     8641 aataccgcag gtgaactggt agtgccgaac ggttcgattg gttttcgctg gggagaaaaa
     8701 ggcaaatgga atctggaatc cattgccgcc ggtacggaaa ccgaattgtt gttaactctg
     8761 ctcggtcaac atgatgccgt tgcaggcgtg gccttcccct actttggcgg cattgaaaat
     8821 ccgcattttc gcagcgtaaa acacaatccg gtcctggtgc gccaattgcc cgttaaaaac
     8881 ctgacattag ccgatggcaa cacctgtccg gtcgtcagcg tttatgattt ggtactggcg
     8941 aattatggcc tcgatcgcgg gctggaagat gaaaacagcg cgaaagatta cgctgaaatc
     9001 aaaccgtaca ccccagcctg gggtgagcaa attaccggcg tgccgcgcca gtatattgaa
     9061 accatcgccc gtgaatttgc cgataccgcc cataaaacgc atgggcgctc gatgattatc
     9121 ctcggtgctg gcgtcaacca ttggtatcac atggacatga actaccgtgg gatgatcaac
     9181 atgctgatct tctgcggttg tgtcgggcaa agcggtggcg gctgggcaca ctatgtcggt
     9241 caggaaaaac tgcgcccgca aaccggctgg ttgccgctgg cctttgcgct cgactggaac
     9301 cgaccaccgc gccagatgaa cagcacctcg tttttctaca atcattccag ccaatggcgc
     9361 tatgaaaaag tctttgctca ggagttactt tcaccgctcg ccgatgccag taagtacagc
     9421 ggtcatctga ttgatttcaa cgttcgtgcc gaacgtatgg gctggctacc ttctgcgccg
     9481 cagctggggc gtaacccgct cgggattaaa gctgaagccg acaaggccgg attatccccc
     9541 acagaattta ccgcccaggc gctgaaatcg ggcgatttac gcatggcctg cgaacaacca
     9601 gatagcggca gcaatcatcc gcgtaatttg tttgtctggc gttctaacct gcttggctcc
     9661 tccggcaaag gccacgagta tatgcagaag tatctgctgg gtacggaaag tggcattcag
     9721 ggggaagaac ttggaccgac ggagggaatc caaccggaag aagttgagtg gcaaactgca
     9781 gcgattgaag gcaagctcga cctgctggtg acgcttgact tccgcatgtc cagcacctgc
     9841 ctgttctccg atatcgttct gcccaccgcc acctggtacg aaaaagacga tatgaatacc
     9901 tcggatatgc atccgtttat tcatccgctt tctgcggcgg tcgatccggc gtgggaatca
     9961 cgcagcgact gggaaatcta caaaggtatc gctaaagcct tttcgcaagt gtgcgtgggg
    10021 catctcggca aagaaaccga catggtatta caaccactgc tgcatgactc cccggcagag
    10081 ctttcacagc cgtgtgaagt gctcgactgg cgcaaaggtg aatgcgatct gattccaggc
    10141 aaaaccgcgc cgaatattgt ggcggtggag cgcgactacc ctgctaccta tgaacgcttt
    10201 acctcactcg ggccattgat ggacaaactt ggcaacggcg gtaaagggat ttcgtggaat
    10261 acccaggatg aaatcgattt cctcggtaaa ctcaattaca ccaagcgtga tggcccggca
    10321 caggggcgtc cgctgattga taccgcgatt gacgcatcag aagtgattct ggcgctggca
    10381 ccggaaacca acggccatgt tgcagtcaaa gcgtggcaag cgctgggcga gatcaccgga
    10441 cgcgaacata cccatctggc gcagcacaaa gaggacgaga agattcgctt tcgcgatatt
    10501 caggcgcagc cgcgtaaaat tatctccagc cccacatggt ctggtctgga aagcgatcac
    10561 gtttcctata acgcgggata caccaacgtt catgagttaa ttccgtggcg cacgctgtcg
    10621 ggacgccagc aactctatca ggatcatccg tggatgcgtg cttttggtga aagtctggtg
    10681 gcttatcgcc cgcctatcga cacccgcagt gtcagtgaaa tgcgccagat accgccaaac
    10741 ggcttcccgg agaaagccct taacttcctg acaccgcacc agaaatgggg cattcactca
    10801 acctacagtg aaaacctgct aatgctgacg ctctctcgcg gtggaccgat tgtctggatc
    10861 agcgaaaccg atgcccgtga actgaccatt gtcgataacg actgggtgga agtgtttaac
    10921 gccaatggcg cgctgacggc ccgcgcggtg gtcagccagc gtgtaccacc gggcatgacc
    10981 atgatgtatc acgctcagga acgcattatg aatattccag gttcggaagt aaccggcatg
    11041 cgcggcggca ttcataactc ggtcacccgc gtttgtccga aaccaacgca tatgattggc
    11101 ggttacgcgc agctggcctg gggctttaat tactacggca ccgtcggctc gaaccgtgac
    11161 gaattcatca tgatccgcaa gatgaagaac gttaactggc tggatgatga aggtcgcgat
    11221 caggtacagg aggcgaaaaa atgaaaatac gttcgcaagt cggcatggtg cttaacctcg
    11281 ataaatgtat cggctgccat acctgttcgg tgacctgtaa aaacgtctgg accggacgcg
    11341 aaggcatgga gtacgcatgg tttaacaacg tcgaaaccaa accgggcatt ggttatccga
    11401 aaaactggga agatcaggaa gaatggcaag gcggctgggt gcgtgatgtg aatggcaaga
    11461 tccgcccgcg tctgggcagc aagatgggcg taataaccaa aatcttcgcc aacccggtgg
    11521 tgccgcagat tgatgattac tacgaacctt tcaccttcga ctacgaacat ttgcatagcg
    11581 caccggaagg caaacatatc cctactgctc gcccgcgttc actgattgac ggcaaacgga
    11641 tggacaaagt gatctggggg ccaaactggg aagaactgct gggcggcgaa tttgaaaaac
    11701 gcgctcgcga ccgcaacttc gaggccatgc aaaaggagat gtacgggcag tttgaaaaca
    11761 ccttcatgat gtacctgccg cgcctgtgcg aacactgcct caatcccagc tgcgtggcga
    11821 cctgcccaag cggtgctatc tacaaacgtg aagaagacgg cattgtgctg attgatcagg
    11881 ataaatgccg tggctggcgt ttgtgcatta gcggttgtcc gtacaaaaaa atctacttca
    11941 actggaaaag cggcaagtca gaaaaatgta tcttctgtta cccacgaatt gagtcaggcc
    12001 aacccaccgt gtgctcagaa acctgcgtgg gtcgcatccg gtatctgggc gtgctgcttt
    12061 acgacgccga ccgcatcgag gaagcggcga gcaccgagcg cgaagttgac ctctatgaac
    12121 gtcagtgcga agtgttcctc gatccgcacg atccctcagt gatcgaagaa gccctgaaac
    12181 aaggtattcc acaaaacgtg attgaagctg cccagcgttc gccagtctac aaaatggcga
    12241 tggactggaa actggcgtta ccgctgcacc ctgaataccg caccctgccg atggtctggt
    12301 acgttcctcc gctgtcaccg attcagtctt acgctgatgc gggcggtttg ccgaaaagcg
    12361 aaggcgtgct gcctgctatc gaaagcctgc gtattccggt gcaatatctc gccaatatgt
    12421 tgagtgctgg cgataccggt ccggtactgc gggcgctgaa acggatgatg gcgatgcgcc
    12481 actatatgcg ttcacaaacc gtggaaggcg ttaccgatac tcgtgccatc gacgaagtag
    12541 gcctgaacgt cgcccaggtc gaagagatgt atcgctacct cgccattgcc aactatgaag
    12601 accgttttgt cattccaacc agtcatcggg aaatggcggg cgatgccttt gctgaacgca
    12661 acggctgcgg ttttaccttt ggcgacggtt gccacggctc ggacagcaaa ttcaacctgt
    12721 tcaacagtag ccgtatcgat gccatcaaca tcaccgaagt gcgcgacaaa gcggagggcg
    12781 aataatgcag atcctcaaag tgatcggcct gttgatggag tatccggacg agctgttgtg
    12841 ggaatgtaag gaggacgcgc tggcgttgat ccgccgcgac gcaccgatgc tcaccgattt
    12901 tgcactcgac ctgctcaacg cgccgctgct ggataaacag gccgaatggt gtgaagtgtt
    12961 tgaccgcggg cgcaccacgt cgctgctgct gtttgaacat gttcatgccg agtcccgcga
    13021 tcgcggccag gcaatggtgg acctgctggc ggagtatgaa aaggtcggtc tgcaactgaa
    13081 ttgtagggaa ctgcccgatt atctaccgct gtatctggag tatttaagca tacttccgga
    13141 cgatcaggcg aaagaaggat tactcaacgt tgcgccgatc ctcgccctgc ttggcggacg
    13201 cttaaaacaa cgcgaggcac cgtggtacgc gttatttgac gccctgttgc aactggcagg
    13261 aagccctcta tcaagtgaca gtgtcacaaa acagattcgc agtgaagagc gtgacgacac
    13321 ccgccaggcg ctggatgcgg tgtgggaaga agaacaggtg aagtttattg aagataacgc
    13381 cacggcgtgt gacagctcgc cgcttaatca atatcagcga cgctttagcc aggatgtcgc
    13441 gccgcagtat gtcgacatca gtgcgggagg tgggaaatga ttcagtatct gaacgtcttt
    13501 ttttacgata tctacccgta cttgtgtggc acggttttta tccttggaag ctggctgcgc
    13561 tacgattacg ggcagtatac ctggcgtgcc gcctccagcc agatgctgga taaacgcggg
    13621 atggtgctat ggtcgaactt gttccatatc ggcattctgg ggatcttctt cggtcatctg
    13681 ttcggcatgt taacgccgca ctggatgtac gcgtggtttc tgccggtggc ggtaaaacag
    13741 cagatggcga tgatcctcgg cggaatatgc ggggtattga cgctgattgg cggtgcgggg
    13801 ctactggtgc gtcgtttaac aaatccgcgt atcagggcga cgtcttctgg tgcggatatc
    13861 ctgatcctcg ccattttgtt aattcagtgc attcttgggt taaccaccat tcctttttca
    13921 gcacaacatc cggacggcag tgaaatgatg aagctggtgg gctgggcgca aagcattgtc
    13981 actttccagg gcggtgcatc tacgcatctt gatggtgtcg cctttatatt ccgcgttcat
    14041 ctggtgcttg gaatgaccat cttcctgctc ttcccgttca cccgactggt gcatgtctgg
    14101 agcgcgccgt ttgagtactt tactcgtcgg tatcagattg tgcgttcgcg gcgttaattc
    14161 tcacgcgggc cggacagccc ttcgctctcc ggccacttgc aggctatgat agttctccag
    14221 ccacaggaga acattaatga aaccgcaggt ttaccacgtc gatgctttta cctctcaacc
    14281 gtttcgcggc aattctgccg gtgttgtttt ccccgccgat aatctcagtg aagcgcaaat
    14341 gcagcttatc gcccgcgagt taggtcattc ggaaactgct tttctgctgc acagcgacga
    14401 cagcgatgtg cgcatccgct actttacgcc aacggttgaa gttccgattt gtggtcacgc
    14461 gacagtagct gcgcactatg tacgtgcaaa ggtgttaggt ttaggaaatt gcacggtctg
    14521 gcaaacatcg ctggcaggaa aacatcgcgt gactatcgaa aagcacaacg atgattatcg
    14581 tatttcgctg gaacaaggta cgccgggctt tgagtcaccg ctggaaggtg aaacacgtgc
    14641 ggcgattatc aacgcgcttc atctcactga agacgatatt ctgccaggct tgccaattca
    14701 ggtggcaacc acaggacact caaaagtgat gatcccactg aaaccggaag tggatatcga
    14761 cgccctttcg cctgacctta atgcgctgac cgctatcagc aaacagattg gctgcaatgg
    14821 tttcttcccg ttccagatcc gcccagacaa aaacgaaacc gacggtcgta tgttctcgcc
    14881 tgccattggt attgtggaag atccggtgac tggcaatgcc aatggcccga tgggtgcatg
    14941 gttagtacat cacaacgtat tgccccacga tggcaacgtg ttgcgtgtta aaggccatca
    15001 ggggcgcgca ttagggcgtg acggcgtgat tgaagtgacg gtgacaattc gtgataacca
    15061 accggagaaa gtcaccattt ctggcgtggc ggtgattttg tttcatgccg aatgggcaat
    15121 tgaactctga aaccgttgcc tgatgcgctt cgcttatcag gtctacaacc gggctaatgt
    15181 aggccggata aggcgtttac gccacatccg gcaacctgac ataaattatt ttcccgcctc
    15241 cgggtgcgta tcaaacgccg ccatcaccag cgctaactca tccacagtaa agccatgttt
    15301 cgcatcatct acgcccagac caaactgttc ctgcatcacg gcatataacg acgccacatc
    15361 tggtagattt cgctgctcaa ctgcgtgccc attttcataa tgggtaaaat gaaaattggt
    15421 cagcgttagc ttgccaccgt ccggcaaatg gcgacacatc agcaaatgat ggcgaaaatg
    15481 cgactgcggc cagtgcgccg accagaaatt gcccatcaca taatcgcttt gttgctgctc
    15541 gcagagatca aaacggtaca tcgactgcca gtgctgatga tgattaaact gcaacaccca
    15601 atcatcaccc tcttgcaaca accgatactc tccgtgtggc gtggtttgca tgatatccgg
    15661 cattaaacga atcggcgcgg ttagcgtctg cccaccgaaa ccgacatcag caatccattt
    15721 ttccccttcc agttccacca acagcaaacg atgggtgcgc ggcggtaatg ctggcggatt
    15781 tgataacact acgcgcccta acaagctgcg aacgttaaac cccagctcgc gtaacacccg
    15841 ctcaaacacg ccattctgct caaaacagta accaccacga cgggctatca ccagtttctc
    15901 ttccagcgat tgatcatcaa gctgtatttc cctcggcagc aaaacgtcga ggttttcaaa
    15961 cggaatggtg caattgtgtt tcaggtgcaa tgcacgaagc gtatcaatat tggccgcagc
    16021 agctcccgac cagttaatac gggcaaaata atgattcaga atgggcgtca tcaggcgttt
    16081 cccttagtgg ttttctcaac ttataacctg gaatagacgc cgcaccggct attttgtgcg
    16141 atcgcggaca tgcatcacgt tcgcaaatcg ccaatcccgt taatttcgtt acttattgcg
    16201 ttacgagcta cgcttttaaa acacccttca tagcgaggcg atatcgtgag ccaattcatt
    16261 cccgtcgaat tacaccatgc cagccgtctg ttaaatcatg gcccaactgt cttgatcacc
    16321 agtttcgatg agcaatccca gcggcgtaac attatggctg cagcctggtc aatgccggtg
    16381 gagtttgaac ctccacgcgt ggcgattgtg gtagataagt cgacatggac cagggagttg
    16441 attgagcgta acggtaaatt tggcatcgtt atcccaggcg ttgcagcgac taactggacg
    16501 tgggcagtgg gaagtgtatc tgggcgtgaa gaggataaat tttattgcta tggcattccg
    16561 gtggtgaaag gtccggtgct tggtttgcca cttgttgaag agaaatgtct ggcgtggatg
    16621 gagtgccgat tgctaccagc gacttccgcg caagagaaat acgacacgct gtttggcgaa
    16681 gtggtatcag cagcggcaga cgcacgggta tttgtcgaag gccgctggca gtttgatgat
    16741 gataagctca atacgttgca tcatttaggc gctggcacgt ttgttaccag cggcaagcga
    16801 gtcaccgcgg gctaaagaat catatgcagc aaccaagttg cttaagcaat gtttcaatat
    16861 gagttaaaag catattttat ctcacgattg catattttta gtgaagatta atttatcctc
    16921 accaacatca tagtaattgc gtattatagc ttcattgata aagccctgtt atgtataaaa
    16981 tgcgcgggcg gcactaaaat cgacatagct ggaggtctct actaaaagta gtcttgcctg
    17041 tttaattgtg agctcctttt ctatttcgtt aatcagcaga tgtcccactc cctggcgctg
    17101 atgggaagga ctgatccagt gcattaataa attccagaca tcatttgtca tcacttcagg
    17161 agcgcaatag gcaatccctg ccagtcctaa gtgttctgca ctaaaccata aatcggcaga
    17221 ttgacctgag aaataatgtg ttagtgtttt ttgtacatga tgaaggccat cttgattaaa
    17281 ttgtccactg gcaagcagca tatccagtaa tggttcaaca tctgaaggtt tgactggtcg
    17341 tataatcatg gaattcctta tctattgatg aatgtttgaa aattgtttgc gtggaacatg
    17401 gttaatctga gccaatattt ttgagaaaga atagatgggt atttatataa gcaaagaagc
    17461 catctcttta aatatcaatt aaagtataac attaacacca gacttcattg cgacaggaca
    17521 cgacaaagca cttttgcggt tttttcaggg tgcaattaca aaaattgaat cccttatgga
    17581 ctaatgtgag aaaattccgg ggcatgacta ttttgatata gtggacaatc aagcgtctcg
    17641 aattaacaat gacttttcag atgctaaacc atgcaattgg aagtatgtcg gatataggac
    17701 aacattagga aatcggacag aaaattcatc ataataaaat gacaacttat ctctttctct
    17761 cactttctga gaaatttcac aaaagtataa actgagccaa tatgccttta tatataaaaa
    17821 atactcatgc ttatttttat cggaaaaagc caagattttg tcaagg
//
LOCUS       DAZKGF010000100        17444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZKGF010000100 DAZKGF010000000
VERSION     DAZKGF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.100"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..585
                     /locus_tag="ACHOQV_003166"
     CDS             <1..585
                     /locus_tag="ACHOQV_003166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9631500.1"
                     /translation="ISYELTPQEYNAIELLNSNNLMWGTYNLKKLFGPAIRGKRLKNA
                     DRIPGSLPFVTAGEGNEGISAFISNDVVVFPENTITIDMFGSAKYRNYEYGADDHVAV
                     VHTEFFDRNAVIFITASIHKSSCTGKFNYGRNFYAKDADELMVSLPSKDNNPDYSFME
                     TLILAVHKLVIKDVVQYTDKKLVAYRQITSEKVV"
     gene            937..1062
                     /locus_tag="ACHOQV_003167"
                     /pseudo
     CDS             937..1062
                     /locus_tag="ACHOQV_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            1103..1178
                     /locus_tag="ACHOQV_003168"
     tRNA            1103..1178
                     /locus_tag="ACHOQV_003168"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1136..1138,aa:Ile,seq:cat)
     gene            1186..1262
                     /locus_tag="ACHOQV_003169"
     tRNA            1186..1262
                     /locus_tag="ACHOQV_003169"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Arg,seq:tcg)
     gene            1276..1352
                     /locus_tag="ACHOQV_003170"
     tRNA            1276..1352
                     /locus_tag="ACHOQV_003170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1310..1312,aa:Arg,seq:tct)
     gene            1444..2403
                     /gene="stxA2b"
                     /locus_tag="ACHOQV_003171"
     CDS             1444..2403
                     /gene="stxA2b"
                     /locus_tag="ACHOQV_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFP9631501.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            2416..2679
                     /gene="stxB2b"
                     /locus_tag="ACHOQV_003172"
     CDS             2416..2679
                     /gene="stxB2b"
                     /locus_tag="ACHOQV_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFP9631502.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
     gene            3185..5095
                     /locus_tag="ACHOQV_003173"
     CDS             3185..5095
                     /locus_tag="ACHOQV_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9631503.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAACIYNDIIPADHCLHDVEDMTGLNHPKADLSKGQYGC
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGTDGTYSDTTGASESSTRWGVGR
                     PLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFNGTPANHAARFTEVVDQYRADLAD
                     MAGQCVGGSADSVPWICGDTTYFWKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPDIGYYGSKWRTDRSTWTSQDRASHFSSWARRGIISDRLATAIL
                     SCAGKSSAFVNGTAGVVVPDRPVTTSESVIFYDAKKASDNQLKPYGWDGMDGRRTLVD
                     DSGNKALRIEKNNSAKSWSMYCDIAADKAKLLLEKGGEIAVRFKIPENVNLETTRNKY
                     AFGLYWRIAEWPGEGGEGYLSSFFVQTDKASIDVAYHHTVNQQKELGTFGAFDHDWHT
                     LAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLIDT
                     VEVKANKAGAAA"
     gene            5235..5417
                     /locus_tag="ACHOQV_003174"
     CDS             5235..5417
                     /locus_tag="ACHOQV_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9631504.1"
                     /translation="MIFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            5443..5715
                     /locus_tag="ACHOQV_003175"
     CDS             5443..5715
                     /locus_tag="ACHOQV_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9631505.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            5792..6007
                     /locus_tag="ACHOQV_003176"
     CDS             5792..6007
                     /locus_tag="ACHOQV_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9631506.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            6012..6545
                     /locus_tag="ACHOQV_003177"
     CDS             6012..6545
                     /locus_tag="ACHOQV_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9631507.1"
                     /translation="MSRKFRYGLSAVVLAMIAAGASAPEILDQFLDEKEGNRTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKEKCAQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCLPSTFYKRLNAGDRKGACESIRWWIKDGGRDCRTRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            6542..7009
                     /locus_tag="ACHOQV_003178"
     CDS             6542..7009
                     /locus_tag="ACHOQV_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9631508.1"
                     /translation="MRRVTAIIYALVICIIVCLLWAVNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCPGS
                     VREAPTTSGVDNATGPRLADTVERDYFTLRERLMMMQKQLEGAQEYIRTQCLK"
     gene            7312..8118
                     /locus_tag="ACHOQV_003179"
     CDS             7312..8118
                     /locus_tag="ACHOQV_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP9631509.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            8099..9805
                     /locus_tag="ACHOQV_003180"
     CDS             8099..9805
                     /locus_tag="ACHOQV_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP9631510.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            9805..11949
                     /locus_tag="ACHOQV_003181"
     CDS             9805..11949
                     /locus_tag="ACHOQV_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP9631511.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            12107..13114
                     /locus_tag="ACHOQV_003182"
     CDS             12107..13114
                     /locus_tag="ACHOQV_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631512.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            13138..14352
                     /locus_tag="ACHOQV_003183"
     CDS             13138..14352
                     /locus_tag="ACHOQV_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFP9631513.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            14408..14797
                     /locus_tag="ACHOQV_003184"
     CDS             14408..14797
                     /locus_tag="ACHOQV_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631514.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            14847..15308
                     /locus_tag="ACHOQV_003185"
     CDS             14847..15308
                     /locus_tag="ACHOQV_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631515.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFCRRVAETEQHGGTE"
     gene            15292..15855
                     /locus_tag="ACHOQV_003186"
     CDS             15292..15855
                     /locus_tag="ACHOQV_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631516.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPSRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            15855..16505
                     /locus_tag="ACHOQV_003187"
     CDS             15855..16505
                     /locus_tag="ACHOQV_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFP9631517.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPAPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            16502..>17444
                     /locus_tag="ACHOQV_003188"
     CDS             16502..>17444
                     /locus_tag="ACHOQV_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFP9631518.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKDSAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAITAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDIGAQGAVGPAGPRGEKGEQGERGPQGIPGLKGD"
BASE COUNT         4546 a         3975 c         4845 g         4078 t
ORIGIN      
        1 ataagctatg agcttacacc acaggaatat aacgcgattg agctattaaa tagcaataat
       61 cttatgtggg gcacttataa cctcaaaaag ttatttggcc ccgcaataag aggtaagcgt
      121 ctgaaaaacg cagatcgtat acccggctcc ttgccttttg ttactgcggg tgaaggcaat
      181 gaaggtattt ctgcatttat cagtaatgat gttgttgtat tccctgaaaa taccattacg
      241 atagatatgt ttggctccgc aaaatacaga aattatgagt atggtgcaga tgaccatgtg
      301 gcagttgttc atacagaatt ctttgacaga aatgccgtta ttttcataac agcatcaatt
      361 cataagtctt cttgtaccgg aaagttcaac tacgggagaa atttctatgc aaaagatgcc
      421 gacgagttaa tggtatcttt gccgtcaaaa gataataacc ctgattattc tttcatggag
      481 acgctaattc tagcagtaca taaactggtc atcaaagatg ttgtccagta cactgataaa
      541 aagctggtag cgtacaggca gattacatct gagaaggttg tttagtgcaa atccccacct
      601 gacagggtgg ggaatggtca ggttatcgtg ccctgatgca gaaaaacagt ttacaatcgt
      661 aaaaatcatc atatcatgac aaaagtggtt acattgccac gctgcttaac ccgccgatgc
      721 gcgggttttt tgtatccaga atcctgtaag ttatacggaa agtacacaga aaggaaggtg
      781 cgaccgcaat taataacaaa atcttaaaaa tcgcatatgg cactattagt tttctaaata
      841 ttgtatattt taagtattgc aggataaccc tgtaacgaag tttgcgtaac agcattttgc
      901 tctacgagtt tgccagcctc ccccagtggc tggctttttt atgtccgtag cgtcaaagca
      961 gcaatggcgc tggggcgtcg tgcaactggc gttgagctgg agactgaacg ttttgagcag
     1021 acggtcaggg aagttcagga tttagccagt cagaacggat gatattgcag gattagttac
     1081 gtaccgttat tatcctgcgc ccggcccttt agctcagtgg tgagagcgag cgactcataa
     1141 tcgccaggtc gctggttcaa atccagcaag ggccaccatc acataccgcc attagctcat
     1201 cgggacagag cgccagcctt cgaagctggt tgcgcggggt tcgagtcctc gatggcggtc
     1261 cattatctgt acactgcgtt gttagctcag ccggacagag caattgcctt ctaagcaatc
     1321 ggtcactggt tcgaatccag tacaacgcgc catacttatt ttttctggct cgcttttgcg
     1381 ggcctttttt atatctgcgc cgggtctggt gctgattact tcagccaaaa ggaatacctg
     1441 tatatgaagt gtatattgtt aaaatgggta ctgtgcctgt tactgggctt ttcttcggta
     1501 tcctattccc gggaatttac gatagacttt tcgacccaac aaagttatgt ctcttcgtta
     1561 aatagtatac ggacagaaat atcgacccca cttgaacata tatctcaggg gaccacatcg
     1621 gtgtctgtta ttaaccacac cccaccgggc agttattttg ctgtggatat acgagggctt
     1681 gatgtctatc aggcgcgttt tgaccatctt cgtctgatta ttgagcaaaa taatttatat
     1741 gtggccggat tcgttaatac ggcaacaaat actttctaca ggttttcaga ttttgcacat
     1801 atatcagtgc ccggtgtgac aactgtttcc atgacaacgg acagcagtta taccactctg
     1861 cagcgtgtcg cagcgctgga acgttccgga atgcaaatca gtcgtcactc actggtttca
     1921 tcatatctgg cgttaatgga gtttagtgga aatgccatga ccagagatgc atccagagca
     1981 gttctgcgtt ttgtcactgt cacagcagaa gccttacggt tcaggcaaat acagagagaa
     2041 tttcgtctgg cactgtctga aactgctcct gtttatacga tgacaccgga agaagtggac
     2101 ctcacactga actgggggag aatcagcaat gtgcttccgg agtttcgggg agagggtggt
     2161 gtcagagtgg ggcgaatatc ctttaataat atatcagcga tactgggcac agtggcggtt
     2221 atactgaatt gccatcatca gggggcgcgt tccgttcgcg ccgtgaatga agagatacaa
     2281 ccagaatgtc agataactgg cgacaggcca gttataagga taaacaatac gttatgggaa
     2341 agtaataccg cagctgcttt tctgaatcgc agggctcact ctttaaatac atccggagaa
     2401 taacaggagt taaatatgaa gaagatattt gtagcggctt tatttgcttt tgtttctgtt
     2461 aatgcaatgg cagctgattg tgcaaaaggt aaaattgagt tctctaagta taatgagaat
     2521 gatacattca cagtaaaagt ggccgggaaa gagtactgga ctaaccgctg gaatctgcaa
     2581 ccgctactgc aaagcgcaca gttaacagga atgacggtaa caatcaaatc aaatacctgt
     2641 gcgtcaggtt caggatttgc tgaagtgcag tttaattaat atcagaagca ttgctggttt
     2701 cgtggtgtgc agcaatgtag ttacagtgta atcaatgtca caattcagtc agttgaaggt
     2761 tgtctgcccg actgagaatt tgttaaaaaa atcctgcatg gtgaatcccc ctgagcggag
     2821 gggcatatca gcgtcacagg tgtttctgtt ttacctctat cctttctgtg cgggttcagg
     2881 tgctgatact gaactcaccg ggaggcaccc ggcaccatgc atgaacggta catagcgcat
     2941 acaccagccc ctctccggag gggctttctt gtgggcaaaa aaaagcccga gtgggttcgg
     3001 gcaatagcat gagatataca tttttataat cgaatggatt ttaaccagga ttcataaggc
     3061 tgcgcaactg cgcggccttt ttcgtattgc gggctgtagt cttcctccag tcattgtcct
     3121 gtaacttccg gacttcagcc cgccccttat ctgactcaca acattatccc ggccgggagg
     3181 attcatggca tttaaacact atgacgtggt cagggcggcg tcgccgtcag accttgcgga
     3241 acgaataact caaaaactga aggaagggtg gcagccttat ggtagtgcgc tgatttcgac
     3301 agctggttat ggtgcggagt tcatccagcc agttgtgagt gaggggagca tctcatcacc
     3361 agaggagcca ggcaaccgtc cgacgacctc agcgccttct gttgcgccag aatattacta
     3421 tgtgatcgcg cttgctggtc agtccaatgg tatgtcatac ggtgagggac tgccattacc
     3481 ggatacattc gacagccctg atccacgtat taaacagtta gcgcgtcgca gtacggtgac
     3541 accgggaggt gctgcatgta tctataacga tattatcccg gcagatcact gtctccatga
     3601 tgtggaggat atgacagggc ttaatcatcc caaagcggat ttatcgaaag gtcagtacgg
     3661 ctgtgtggga caggggctgc atatcgccaa aaagcttctg ccatttatac cggcgaatgc
     3721 gggcattctt cttgttccgt gctgccgtgg tggttcagct ttcaccaccg gaaccgatgg
     3781 cacatacagt gacactactg gcgcctcaga gagttccacc cgctgggggg tgggcaggcc
     3841 gctgtataag gacctcattg gtcgtacaaa agccgcgctg gcgaagaatc cgaaaaatgt
     3901 gctgcttgcc gtggtatgga tgcaagggga atttgatttt aacggaacgc cagcgaatca
     3961 tgcagcccgt tttacagaag tggtggatca ataccgtgcg gaccttgcag atatggcggg
     4021 gcagtgtgtg ggtggctcag ctgacagtgt tccctggatt tgtggagaca caacatattt
     4081 ctggaagcag aagaacgaaa cggcgtacca gacggtgtat ggtagttaca aaaataaaac
     4141 ggaaaagaat atccatttcg tgccgttcat gaccgatgag aacggggtga atgtgccgac
     4201 gaacaaaccg gaagaagacc cggatattcc ggatatcggg tattacggtt caaaatggcg
     4261 aacggacaga agcacctgga catctcagga cagggccagt catttcagtt catgggctcg
     4321 ccgtgggatt atttccgacc gtctggcaac ggcgattctg agctgcgcgg gtaagtcttc
     4381 tgcgtttgtt aatggtactg ccggggtggt tgttccagac agaccggtta ccacctcaga
     4441 gtctgtaatt ttttacgatg ccaaaaaagc ttcagacaat cagctgaaac cttatggctg
     4501 ggacggtatg gatggcagac gcacactggt tgatgacagc ggcaataaag ctctgcgaat
     4561 tgagaaaaat aacagcgcga aatcctggtc aatgtactgt gatattgctg cagacaaggc
     4621 aaaactttta ctggaaaaag gcggggaaat tgctgtccgg tttaaaatcc ccgaaaacgt
     4681 caatcttgag acaaccagaa acaagtatgc ctttggtttg tactggcgaa tagcggaatg
     4741 gccgggtgag ggtggtgaag gctatctgag ttctttcttt gtccagacag ataaagccag
     4801 tattgatgtt gcataccatc atacagttaa tcaacaaaaa gaacttggca cgtttggcgc
     4861 attcgaccat gactggcata cgcttgcatt taaatttaag ggcagtaaca gcattaatgt
     4921 tactccggtg cttgatggtg tggatggaca ggcgtttgac ctggtgaaat gggccaatac
     4981 tgctaatgga ctcaacaggt ttgtcattac ggatattaca ggtagtgcag aaacctaccc
     5041 tgtacttatt gatacggtgg aagttaaagc aaacaaagct ggagcagccg cataattgct
     5101 aaaaaaagcc gccagcggca ggaatggaag ctggcggagg taatcccaat ggagaatgta
     5161 aagaaaagat gctttcgtac attggttttt taaatgaaaa cagttctcat tgtcaaccat
     5221 aacggtaaga aattatgata tttattcatc aggtgatgct gtacttctgt acggcggtct
     5281 gtgtgctgta tcttctttcg ggtgggtaca gggctgtgcg cgatttctgg tgcaggcaga
     5341 ttgataaaag ggccgctgag aaaatcagcg ccagtcagtc agccggaagc aaacccgaag
     5401 agcccgttac tccttaacaa cccctttcag cgagaaaatc ccatgtcaga aatcacatcc
     5461 ctggtcactg cagaggcagt gaaggaagtc ctgcgctctg aagaagtccg gagcgcactg
     5521 aaacagaaac ttcgccagaa tcttgaggcg cgtcttgatg cagaagttga tgccattctg
     5581 gatgagctgc ttggtgtaca ggcagagcca ccgactgaag cgggagatac caccgcagag
     5641 agcggtgaag ttcagcctga atcaccggtc gccgatgcga ctgaacctca acccgaatcg
     5701 gtcatgatgc tgtaacgagg agtcagggcc atcagtaaac ggctgctggc ctttttcatg
     5761 ttgtgagctt ccggattgcg ggagacgggg tatgtaccag atggaaaaaa tcacaacagg
     5821 tgtgtcatac accacgtcag cggtgggaac gggctactgg ttcctgcagt tgctggacag
     5881 ggtttccccg tctcagtggg cggcaatagg cgtgctgggg agtctgctgt ttgggctgct
     5941 gacatatctg acgaacctgt atttcaaaat taaagaagac cggcgtaagg cggcgcgggg
     6001 agagtaagct gatgagcagg aaattccgct atggtttatc agcggtcgtt ctggcgatga
     6061 ttgctgcagg tgcttctgcg cctgaaatcc tcgaccagtt tcttgacgaa aaagaaggta
     6121 atcgcaccac agcataccgt gatggtgcgg gtatctggac catctgccgt ggtgccaccc
     6181 gggtggatgg taagcccgtc gttcccggca tgaagctgtc gaaggaaaaa tgcgcacagg
     6241 ttaatgccat tgagcgtgat aaggcgctgg catgggtgga gaaaaacatc aaagtgccac
     6301 tgaccgaacc ccagaaagcg ggtattgcgt cattctgccc gtataacatt ggccccggta
     6361 agtgtctccc gtcgacgttt tataagcggc ttaatgccgg tgatcgtaaa ggtgcctgtg
     6421 agtcgattcg ctggtggatt aaggacggtg ggcgtgattg ccgcacacgt tcaaataact
     6481 gctacggaca ggttattcgt cgtgaccagg aaagcgcatt agcttgttgg gggatagatc
     6541 agtgagaaga gtaaccgcga ttatttacgc tctggttatc tgcatcatcg tctgtctgtt
     6601 atgggctgtt aatcattacc gcgataacgc catcacctac aaagcgcagc gcgataaaaa
     6661 agccagagag ctggagctgg caaacgcaac cattactgac atgcagcagc gccagcgtga
     6721 tgttgctgcg cttgatgcca gatactcaag ggaattagcc gatgcgagag ctgaaaatga
     6781 aactctgcgc gctgatgttg ccgctggtcg taagcgcctg cggatcaacg ccacctgtcc
     6841 aggctccgtg cgtgaagccc ccaccacctc cggcgtggat aatgcaaccg gcccccgact
     6901 ggcagacaca gttgaacggg attatttcac cctcagagag cggttgatga tgatgcagaa
     6961 gcagctggaa ggggcacagg aatatatccg cactcagtgc ctgaaataag ttttgctgat
     7021 gcgccgtatc gtcgccgtat tcccgtatta acagcgactg cagccctgac gggtacctcc
     7081 tgcgtgagtg tgcggggata atcaaaaacg atacacaccg ggtttccctc atttttcagg
     7141 agacgtgagc atttacccgc gaagccgcct gtccggtgcg gtggtggaag aaaccggata
     7201 aaacaacctc attgtacaaa tatcgatcaa gtatggcgct gctgtgtgaa ttctgaaaaa
     7261 tcacagcggt cattatgcgt cagtttttaa cagaggacgt cagaaagtga catggcaaag
     7321 ctggactgga aaaagctgga gcaggcattc cgacgcgaac atgccgaaac gggaataaca
     7381 ttactggact ggtgccggaa gaaaaagatt aattacaaca ccgccagaac ccgtataaaa
     7441 atgggcaaaa tcgatcatga aattgatcat aaaaccgatc atgaaatcga tcatgacatc
     7501 tcagatgaag aacccagcaa tgacgcgtgt tccgacgatg aaaaatgcgc aaaaaactct
     7561 gaaaaaaact gcgcaaattc ggcagaaacg aaacggattc gtggttcccg acttttacct
     7621 ccttcaaacg ctttttctca gcgaaacacc cacgccgtaa gacaccgtgg atatgcgaag
     7681 tatcttgagg cagataacct catggatgat gcgtccgaca tggtgctgtt cgatgaactg
     7741 gtgttcaccc gggcccgcgc actttcagta actaaggcac ttaaagggat gttcgccgac
     7801 ctggaagagg caactgacgt ggaaacccgc gttgctcttt acgacaaaat actcaaagct
     7861 gaacaggccc ttgaccggaa tattgcccgt atcgagtcaa ttgaacgctc attgctgacg
     7921 ctggacgtcc tggctgagac agcaccaaaa cttcgtgctg accgggaaag aatcaacgcc
     7981 gccagagata aactcagagc ggaaaccgat attctgacca gccagcgccg gggggttatt
     8041 acgccagtca gtgacatcgt gtcatcgctg catgaaatga gtaattcggg gagactggat
     8101 gacattccgg aagaataaaa cgcgatgtga tgaacctgca gaaatgactg agaccgaaca
     8161 acgtctgttc attatgacaa aactgagcaa tccctggtgg cgcctcaatc atctctacaa
     8221 aatacagaac gaaaaaggtg aactggtcac cttcagaatg cgaccggcgc aacgtcagtt
     8281 gtttcgtaac atgcataata aaaacattat cctgaaagcg cgccagctgg gattttccac
     8341 agccattgat atttatcttc tcgaccaggc attattcatt ccgcatctca aatgcgggat
     8401 cgtcgctcag gataaacagg ctgccagtga aattttccgc acaaaaattg ctgtaccgtt
     8461 tgatcatctc cctgactggc tgagagcctc attcaccatc gttgaacgtc gtagcggtgc
     8521 cagcggtggc tatatcctgt ttggtcacgg ctcgagtatt caggtggcaa cctcatttcg
     8581 ctcaggtacg gtgcagcgcc tgcatatctc agagcacggc aaaatttgcg cgaaatatcc
     8641 ggctaaggcg aaagagctgc gaaccggtac gcttaatgcc gtctctgatg aatgcattat
     8701 ttttgatgaa tccacggctg aaggcgtggg tggtgatttt tacgagatga gtaaccgtgc
     8761 acaggagagc actgcatcag gcttattgct gacggcacag gattataaat tccattttta
     8821 cgcatggtgg caggatccta aatacagcgc cagagtgcct gaaagcgggc tgaagctgtc
     8881 acgggaaaaa atgacgtatt tttctgcggt tgagaaggca atgaacatca cgcttaccga
     8941 tgagcagaag cagtggtaca tcagtaagga aactgaacag cgtgaggaaa tgaagcagga
     9001 gtttccctca acgccacagg aggcgtttct gacgtccgga cgacgtgtgt tcagtgccga
     9061 aagtacattg caggcagaat cattctgttc gccaccgatg attgtttatg acattgaacc
     9121 tgttacagga gcgaagacta aagctcagtc tctgcgtgaa ggaaataaaa acgagttgca
     9181 gcggacgctg atgaattatc tgctggtatg ggaactgccg gatccggatg aagagtatgt
     9241 ttgtggggca gatactgccg aagggctgga gcacggagac cgctcatcgc tggatgttgt
     9301 caaacgcagt aatggcgagc aggtggctca ctggttcggg catctcgatg ctgaactttt
     9361 tgctcatctc atttcgcagg tctgtcgtat gtataacaac gcgtttgtgg ggccggagcg
     9421 taataatcac ggacatgcag ttatcctgaa actccgggaa ctctatccga cacgttatat
     9481 ctacaacgaa cagcatcttg accaggcata tgacgacgat acgccccgcc ttggctggct
     9541 gacaacccgt cagagcaaac ctgttctgac cgaaggaatg aaaacgctcc tgaataatgg
     9601 aatatcaggg atccgctggt caggcacatt atcggaaatg aacacctacg tttatgacgc
     9661 gaaaggctcc atgaatgcac aggaaggctg ctttgatgat cagctcatga gctacatgat
     9721 tgcccaggag atgcgcgcca gaatgccggt gagggtaaaa cagaaaacgg ataaacgcag
     9781 aaccacacac tggatggcac actgatgaaa aatgaaatta acaccacagc gatgaaaaac
     9841 gatcatggat ccacgccgcg tttttctcag cgtcagttac tgtctctctg ttctgatatt
     9901 gacagtcagc ctctctggcg tgatgccgca aacaaggcct gtgcgtatta tgatggcgac
     9961 cagctggcac cggaagttat ccaggtactg aaagatcgcg gtcagcccat gaccatccat
    10021 aacctcattg cccccacggt agatggtgta ctgggaatgg aggcaaaaac aagaacggac
    10081 ctgatagtga tgtcagacga tccgaacgat gaaacagaga aactggcaga ggccattaat
    10141 gcggagtttg ctgatgcgtg ccgtcttggc aacatgaata aggcccgctc cgatgcctat
    10201 gcggaacaaa tcaaggcggg gctcagttgg gtggaggtca ggcgcaacag cgatccgttc
    10261 ggacctgaat tcagggtatc cacagtcagc agaaatgagg ttttctggga ctggctcagc
    10321 cgggaatccg acctgagtga ctgccggtgg ctgatgcgtc gccgctggat ggataccgat
    10381 gaggcaaaag ccacgttccc gggaatggct caggttatcg attatgctat tgatgactgg
    10441 cgtggttttg tcgataccac gattactgaa ggccagccca gtccgttgat gagtgcatgg
    10501 gaagagtatc agtcatggga tcgcagggag aatgaatggc ttcagcgtga acgccgtcgt
    10561 gtgctgcttc aggtggttta ttaccgtaca ttcgaacgtc ttccggtgat tgaactcagt
    10621 aatggacggg tggtggcttt tgataaaaat aatctgatgc aggcggtggc tgtggcatcc
    10681 gggcgggttc aggtgaaagt cgggcgggta agtcgtattc gtgaagcctg gtttgtgggc
    10741 ccacacttta ttgtggatcg cccctgtagt gctccgcagg ggatgtttcc gctggttcct
    10801 ttctggggat accgaaagga taaaaccggg gagccatacg ggttaatttc ccgcgccatt
    10861 ccggcacagg atgaggtgaa ttttcgtcgt atcaagctga cctggctgct tcaggccaaa
    10921 cgcgtgatta tggacgagga tgccacccag ttgtcagaca gcgacctgat ggagcagatc
    10981 gaacgtccgg atggcattat taaactgaat ccggcccgaa aaaatcagaa aagtgtcgca
    11041 gatgtttttc gggttgagca ggattttcag gttgccagcc agcagtttca ggtcatgcag
    11101 gaatcggaaa aacttatcca ggataccatg ggagtgtatt ccgcatttct cgggcaggat
    11161 tcaggtgcga cgtcaggcgt ggctatcagt aacctggtgg agcagggggc cacaactctt
    11221 gcggaaatca acgataacta ccagtttgcc tgccagcagg tgggaagact gttgctggct
    11281 tatcttctcg atgacctgaa aaaacgccgt aatcatgcag tggtgattaa tcgcgatgat
    11341 cgccagcgtc gccagaccat tgtcctcaat gctgaaggtg ataatggtga actgaccaat
    11401 gatatttcaa ggttaaatac acatattgcg ctggcgcctg ttcagcagac accggcgttt
    11461 aaggcacagc ttgcacagag aatgtcagag gttattcagg ggctgccgcc tcaggtgcag
    11521 gctgttgtgc tcgacctgtg ggttaatctt ctggatgtgc cgcagaaaca ggagtttgtt
    11581 gagcgtattc gtgctgcgct ggggacgcca aaatcaccgg atgaaatgac gccggaagaa
    11641 caggaagtag cggcacaaca acaggcactt cagcaacaac aggcagaact ccagatgcgc
    11701 gagatggctg gcagagtggc aaaactggaa gctgacgccg ccagggcaca tgcagctgca
    11761 caacgggata atgccagtgc acagcgggaa gtcgccctga cacaggggca gcgttatgtg
    11821 gatgcgctta accaggcaca tacggcagaa atcattaccg gcgtacagaa tatggaacag
    11881 gagcaggacg ttcttcagca acagatgctg tatacgttac aacagcggat gaatgaaatg
    11941 tcgctctgaa aactctggct tcaactgaac cccgtcatcg tacggggttt tttgtttccg
    12001 gaggtaagcg ttccgggagc ggtgcgctta ttcgcggggg cagcgataag ccttatttac
    12061 tcaaccattc ggatctgtcc gataaacaga ccatgcggag ttatttatgg attttgaatt
    12121 tacgggtgaa gaaaccccgg aacaactgga aaaaatgctg gaaggacttg gggatgtgga
    12181 tattgacagt cacgcacagg acgtcgtgac ggaagatacc acggaaaaac atgcggatga
    12241 ggaagcacag actcagacgg gcgataacaa tgtggcaccg acgccggatg ccagtgtgga
    12301 gcagacgcag gacgtgaagg agccggaagc gaagggggtg ctcacccgcg acggtaaaca
    12361 cgtcattccc tatgaagtcc ttgaggctga acgttccggt aagcaacggg ccgaacagga
    12421 agccgcactt cttcgtgggc agatagctga agaaaaacgc agggtggaac tgctgacgtc
    12481 tcagatccac caggccggta tgaagcccac accgttaccg gaaaacgaaa aaatttctga
    12541 tgagcagatt gcccgtatca gggagatgta tccggaaatt ggtgacgcgg tggcttcgct
    12601 catccgtaaa aataactatc tccagtcccg tgttcagcaa tcaacacagc aggcagaagg
    12661 taatggtggt gaggatttat caccggttct tgatgcgatg aatgccgtgc cggtgctgaa
    12721 aacgtggcag gagtccgatc cggatcgctt ctcggttgct gtatccatcg acgggaagct
    12781 ccagaatgac cccgcatgga aagacaaaac gctcactgaa cgtttcgctg aagtggcccg
    12841 tcgtacgcag gttgctttcg gtgaagtcag tgagtcgtct gctgacaacc aggcagacaa
    12901 aacggatatc cggaaaacgg cggaagaaaa agtgaagacc gctgaacagg agcaggcagt
    12961 acctgcttcc ccgtcagatt taggcaccac ggcctccgtc ggaaccggtg ataattttga
    13021 acggttactt ggtgcttctc attcagaggc tgaggcgatt atgcgtggta tgacgaatgc
    13081 tgaaatagac gcacttctgg agaagctcgg gtaacttact gaaggagtac tgaagtaatg
    13141 acgactgtaa catcagccca ggcgaataag ctgtatcagg tggcgctttt tactgctgcc
    13201 aaccgcaacc gctcgatggt taatatcctt actgaacagc aggaagcgcc aaaagcggtt
    13261 tcgccggaca agaaaagcac gaagcagacc agcgcaggtg cgccggttgt ccgtatcaca
    13321 gaccttaaca aacaggccgg tgatgaagtg accttcagca tcatgcacaa actctcaaaa
    13381 cgtccgacga tgggagatga gcgtgttgaa ggtcgtggtg aggatctcag ccatgctgac
    13441 ttctccctga aaatcaatca gggacgtcac ctggtggatg caggcggacg tatgagtcag
    13501 cagcgcacga agtttaacct ggcatcctct gccagaacgc ttctggggac gtactttaat
    13561 gacctgcagg accagtgtgc gatagtgcat cttgccggag cacgtggtga ttttgttgct
    13621 gacgacacta ttctgccgac agcggagcac cctgaattca aaaaaatcat gatcaacgat
    13681 gtactgcctc cgacacatga ccgtcacttt tttggcggtg atgcgacaag ctttgagcag
    13741 attgaagcgg cagatatttt ttctattggc ctggtggaca atctctccct gttcattgac
    13801 gaaatggcgc atccgttaca gccggttcgt ctgtccggtg atgaacttca cggagaagat
    13861 ccatattacg tcctgtacgt cacgccgcgt cagtggaatg actggtacac ctcgacgtcc
    13921 ggtaaggact ggaaccagat gatggttcgt gccgtgaacc gtgcaaaagg ttttaatcat
    13981 ccgctgttca aaggtgaatg tgcgatgtgg cgcaatatcc tggttcgtaa gtatgcgggt
    14041 atgccgatcc gtttctatca ggggtcaaag gttctggtat cagagaataa cctgacggca
    14101 accacgaaag aggtcgctgc tgcaaccaat attgaccgcg ccatgttact gggggctcag
    14161 gcgctggcaa atgcttacgg tcagaaggcg ggcggtcact tcaacatggt tgagaagaaa
    14221 acggatatgg ataaccgtac tgagatagca atcagctgga tcaacggtct gaaaaaaatc
    14281 cgtttccccg agaagagcgg caagatgcag gatcacggcg tgattgccgt tgatacagca
    14341 gtgaagctct gattttttcc tttccccatg ccgggttttc gcccggcttt ttcaggagtc
    14401 attaattatg gcaaagacta tccttgcccc gtcactgagt gaacgggtct atacgggtac
    14461 gcacggtaat gagtcggtgg cagaaggcgt atttacggtg aatgctgcgg aagcggacag
    14521 tgttattcat cttctctcac tgccagtggg catccgtatc aactcactcc agctggtttc
    14581 aacgggtggt ctgggtactg caaccgtcag cattaagtcc ggtgagcatg ctctcatcga
    14641 taacagcgaa gctgtttctg caaaatttgc cagatatgtg ccagtggagc cgtacaccac
    14701 acagcgtgac ggggagctgg ttactgtcac cattaagact gccgctgcaa ccggcaccct
    14761 gaatgttctg ctgcgttata ccgtggtggg atactgatta aaaccttccg gcccgcgtca
    14821 tgcgggcttt ttatccgggg aattatatga gtgagaaaat tgccgttgtc tatatcggcc
    14881 caaaacccgt gaaaaaggac accattaccg gaagccgcac attgttccca cgtcttgagc
    14941 cggtgcatgt tgacagtgcg atggcctggc aactgctggg gtttccggat gtctgggttc
    15001 gtcatgaaga gcttgatgat gttctgaaaa agcaacaaca gaatgagcag ttgcggcagg
    15061 cacagcaggc gcaggaaaga gtgcttgctg cgcgggcaga agcggagaac agttttgttg
    15121 tttctgttaa cgggcaggag gtggatttaa gtaagctcac ctcagcacgg ctggcgacgc
    15181 tgtgtgaggc agaagagctg gatattcaca aagacccgaa agaaacggct gaggcattcc
    15241 gtatccgggt gcgtgaggca ttttgccgtc gtgttgcgga gactgaacag catggcggaa
    15301 ctgagtgatt ttttaccata tgtccgtcgt catatcagcg gtccactgaa cattatgatg
    15361 acggatgctc tgtcaatggc tgccgtggca ttcagccgcc agtcgttggt gtgccgtcgg
    15421 gaggttactg ttgtaccagt agcaggaaaa gaaatcgtgc ttccgtatga caaagatgat
    15481 gaggagtgcg ttcatatcat ccgtatctct gacgataatc atgagctttt tgtcggtcgg
    15541 gatgtggata tcagctccgg acgctccctg cgatttgcct gttctcccgg tgaggtgagc
    15601 gtgctttatg ccgtcgctcc gaaagccgga cgcagccaga taccggatga actcctcaca
    15661 tggcctgaag aagtggctgc gggggcactt gagcggttgt tcatgcagac tggtgtttca
    15721 tggtcagacc cgtcacgcgc acagtatttt tctgtgcagt tttctgaggg gatccgtcgg
    15781 gcatatcgtc atacactggc gacaagcccg tactcttcat accgcaaccc tgtacgcagg
    15841 cagaggtttt actgatgacg acgattacag aaatcatcgg acgtgtgaat acacaactgg
    15901 ttgacccgat gatggttcgc tggcccctgc aggaattgtg cgattattac aatgatgctg
    15961 tgagggcagt gattctggcg agaccggatg ctggcgcaag cctggaaaca atcagttgtg
    16021 ttcctggcgc ccgtcaggtt ttgcccgatg gtgtaataca acttcttgac gtgatatgcc
    16081 tcagtgacgg tagtgcagtc agaccattat cccgggaggt gctggatgcg cagtatcccg
    16141 agtggcccac aatgaagggc attcctgaat gttttatcag caacgacctg tccccgcgcg
    16201 tattctggct gtttcctgcc cctgacaaag agataagtat tgatgcggtg gtaagccgga
    16261 taccggaggc agtgtatgtt ctgacgcagg acgatgatac gccagttcca ctggaagagg
    16321 cttatgttaa cccactggtg gactggatgt tgtttcgtgc tttcagtaag gatgctgccg
    16381 gtggtgcaga atcggggctg gctgcgcagc attatcagag ttttgttgag caacttggga
    16441 tcaaacaggg ggcagacagt gcattgtctg cccgtaaaaa agtgtttaac ggaggtggag
    16501 tgtgagtgtt gttgtttcgg ggacgctgaa atctcctgat ggtgaggcga tatcaggagc
    16561 aaatattacc ctgacggcgc tgacagtttc accggatgcg ctcagcggca ccagtgcgtc
    16621 ggcagtgacc cgtgaaggtg gatattacgg aatgacgatg gatccggggg agtatgcggt
    16681 ttcggtgacg gtgaaaggga agactgctgt ctacggacgt gtgcgtattg aggggaccga
    16741 aagtacggtg acgctcaata tgctgttacg ccgcagtctt gttgaggtga gcatacccgg
    16801 agagctgctg acagatttcc ggcagataca gaataatgtg gctgatgacc ttgccactat
    16861 tcgtcgcctg aatgaagaca cggcgacaaa aaacactcag gccacacagt caaaagacag
    16921 tgcagcagcc agtgcgaaga gtgcatctga cagtgcaaag acggcaacca gcagggcggc
    16981 tgaagccgga caaaaagcga ctgatgctac tgaggccgcg acccgtgcaa tcacagcagc
    17041 ggggaatgca gaggaaagct cgacccgtgc cggagagtct gaaaaagccg ccggagctga
    17101 tgcagaaaaa gccagacagc atgctgaaaa ggccagactg gcgcaggaga gcgccggaga
    17161 gatccttaag cgggcagagg ctgccactgt cagtgctgaa gaggccagac gtatggctga
    17221 gaatgcacgg gggccccggg ggcctcaggg agaaactggt ccgaaggggg atgtcggtcc
    17281 taaaggcgaa acaggtccag tgggccctca agggcccgca gggccgaaag gtgagcgtgg
    17341 tgacattggt gctcaggggg ctgtagggcc cgctggtccg cgtggtgaga agggcgaaca
    17401 gggggagcga ggaccgcagg gaataccagg cctgaagggg gata
//
LOCUS       DAZKGF010000101        17365 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZKGF010000101 DAZKGF010000000
VERSION     DAZKGF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.101"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..466)
                     /gene="yehS"
                     /locus_tag="ACHOQV_003189"
     CDS             complement(<1..466)
                     /gene="yehS"
                     /locus_tag="ACHOQV_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP9631519.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKK"
     gene            complement(513..1232)
                     /gene="btsR"
                     /locus_tag="ACHOQV_003190"
                     /gene_synonym="yehT"
     CDS             complement(513..1232)
                     /gene="btsR"
                     /locus_tag="ACHOQV_003190"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFP9631520.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1229..2914)
                     /gene="btsS"
                     /locus_tag="ACHOQV_003191"
     CDS             complement(1229..2914)
                     /gene="btsS"
                     /locus_tag="ACHOQV_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFP9631521.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDCYTRITLRLPWRDEA"
     gene            3136..3867
                     /gene="mlrA"
                     /locus_tag="ACHOQV_003192"
     CDS             3136..3867
                     /gene="mlrA"
                     /locus_tag="ACHOQV_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFP9631522.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            3927..4034
                     /gene="yohO"
                     /locus_tag="ACHOQV_003193"
     CDS             3927..4034
                     /gene="yohO"
                     /locus_tag="ACHOQV_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFP9631523.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(4015..4746)
                     /gene="yehW"
                     /locus_tag="ACHOQV_003194"
     CDS             complement(4015..4746)
                     /gene="yehW"
                     /locus_tag="ACHOQV_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFP9631524.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIIVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKSMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(4751..5677)
                     /gene="yehX"
                     /locus_tag="ACHOQV_003195"
     CDS             complement(4751..5677)
                     /gene="yehX"
                     /locus_tag="ACHOQV_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFP9631525.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLVEA"
     gene            complement(5670..6827)
                     /gene="yehY"
                     /locus_tag="ACHOQV_003196"
     CDS             complement(5670..6827)
                     /gene="yehY"
                     /locus_tag="ACHOQV_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFP9631526.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACVIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHLLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGVWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGISGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(6834..7751)
                     /gene="osmF"
                     /locus_tag="ACHOQV_003197"
     CDS             complement(6834..7751)
                     /gene="osmF"
                     /locus_tag="ACHOQV_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFP9631527.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(7900..10197)
                     /gene="bglX"
                     /locus_tag="ACHOQV_003198"
     CDS             complement(7900..10197)
                     /gene="bglX"
                     /locus_tag="ACHOQV_003198"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFP9631528.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATAKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            10393..12108
                     /gene="dld"
                     /locus_tag="ACHOQV_003199"
     CDS             10393..12108
                     /gene="dld"
                     /locus_tag="ACHOQV_003199"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP9631529.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGVGKTSKRKN
                     WQEVE"
     gene            complement(12147..13079)
                     /gene="pbpG"
                     /locus_tag="ACHOQV_003200"
     CDS             complement(12147..13079)
                     /gene="pbpG"
                     /locus_tag="ACHOQV_003200"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFP9631530.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(13253..13840)
                     /gene="yohC"
                     /locus_tag="ACHOQV_003201"
     CDS             complement(13253..13840)
                     /gene="yohC"
                     /locus_tag="ACHOQV_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFP9631531.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14010..14588
                     /gene="yohD"
                     /locus_tag="ACHOQV_003202"
     CDS             14010..14588
                     /gene="yohD"
                     /locus_tag="ACHOQV_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9631532.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILAVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(14707..15468)
                     /gene="yohF"
                     /locus_tag="ACHOQV_003203"
     CDS             complement(14707..15468)
                     /gene="yohF"
                     /locus_tag="ACHOQV_003203"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9631533.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGARAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVRPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(15521..16957)
                     /gene="mdtQ"
                     /locus_tag="ACHOQV_003204"
     CDS             complement(15521..16957)
                     /gene="mdtQ"
                     /locus_tag="ACHOQV_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFP9631534.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQHTPAVQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPVMQVAEQRIQLAEAQAKAIATQD
                     GPQLDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVDAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17181..17264
                     /gene="yohP"
                     /locus_tag="ACHOQV_003205"
     CDS             17181..17264
                     /gene="yohP"
                     /locus_tag="ACHOQV_003205"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFP9631535.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         4200 a         4801 c         4320 g         4044 t
ORIGIN      
        1 ttttcttcac atgctggcgc gctgccagtc cacgcagaaa ataacgtaaa aattgatcgc
       61 cgcattcgcg gaagttttta tgatccggtg cgcgcatcat cgctgtaatt tccggcatcg
      121 aaacgcggaa ctgctgttcg gtgaggatcg ccagaatgtc atcggttttc agcgaaaacg
      181 cgatgcgtaa ttttttcagc acgatgttgt tattaatgcg acgttccggc tccagtgccg
      241 gagcagactc atccttgccg cgtttttcat aaatcaggcc attgaggaat gacgacaaaa
      301 caatgtccgg acaacgctga aaaccctctt cgtcttcttt acgtagccag acggcgatct
      361 gttccgcggt ggcttcgaca ttacccagcg ccagaatacg caccaggtca ttattattgg
      421 ctttcaaaat gtagcgcacg ctgcgcagaa tatcgttact tagcatgagg ccttcaggtg
      481 ttgatgaggc aaaaagccat tttagcagtc ttttacaggc caatcgcctc ttttaagctt
      541 ttcagataac ggcggctgac cggcacggtt aagccattac gcaaaatcaa ctcggcctgg
      601 ccgttatctt ccagacgaat ctcctgtaaa tgcgcgaggt taaccagata ctgacgatgg
      661 cagcgcagta gtggtgtacg actttccagg gtacgtaatg tcaattcggt aaagccctct
      721 ttcccttcgt ggctggtaac gtagacaccg ctcatccgac tgctgacaaa tgccacatct
      781 ttcatttgca gtaaataaat ccgactatgc cccgtacaag ggataaattt cagcgcctgt
      841 tgattttccg gtaacagcga aacatcctgc ttactgcgtt cctgacgcaa acgcgccagc
      901 gttttctcca gtcgcgcttc atcaattggc ttcagcagat agtcaaaggc atgttcttca
      961 aaggctttaa tagcgtattc gtcaaacgcg gtgagaaaaa cgatatacgg tcgatgttcg
     1021 gggtcgagca tccccaccat ttccagacca ctgatgcgcg gcatctggat atcgagaaac
     1081 agcacatccg ggcgcagttt atgcaccgcg ccgatccctt ctacggcgtt tgaacactct
     1141 ccaacgattt caatatcgct ctgctcctgc aaaaatacgc gcaggttctc ccgtgcaagc
     1201 ggttcatcat cgacaattaa gactttaatc atgcctcgtc cctccatggt agtcgtaacg
     1261 ttattcgggt gtaacaatca ggctcacagg cgacgcttat cccatagtca tcgccaaacc
     1321 gttcacgtaa acgcttatcc accagattca tccccagccc actggcattg gttaccggtt
     1381 gatacaaacc ggcattgtct tcgatctcca gcatcaaatg ttgcccctca cgtcgggcgc
     1441 tgatcgccac tcgccctgta tccagcagtt gtgatgtccc atgtttaatg gcgttttcca
     1501 ctatcggttg cagggtaaac gcgggcaatt gctgctggga taattcttgc ggaatagcaa
     1561 tgttgacctg caaccgcgac tggaagcgcg ccttttcaat ttgcagataa gcgttcacat
     1621 gttcaatttc gtctgcgaga gtaacaaact ccgaaggccg ctttaagttt ttgcggaaaa
     1681 aagtggaaag atactggacc agctggctgg cctgttcgct gtcgcggcgg atcaccgctt
     1741 taatggtgtt aagcgcatta aacaaaaaat gggggttcac ctgggcgtga agcagtttga
     1801 tctctgactg ggtgagcatc gctttttgcc gctcatattg cccggcgagg atctgcgccg
     1861 aaagcagttg cgcaatcccc tcgcccaacg tgcggttgat tgaactgaat aaacggtttt
     1921 tggcttcata caatttgatg gtgcccatca cccgctgatt ttcgccacgc aacggaatga
     1981 ccagcgtcga ccccagtttg cattgcggat gcaaagagca acgataaggt acttcgttgc
     2041 catcagcgta gaccacttca ccggtttcaa tcgcttttaa ggtgtaagtc gaagaaatcg
     2101 gtttgccggg taaatggtgg tcgtcaccaa ttccggtaaa ggccagcaat ttctctcgat
     2161 cggtaatcgc gactgcacca atatccagct cctgatacag cacctgtgcc actttcatgc
     2221 tgttcacttc gttaaacccc tggcgcaaaa tgccttccgt cgaggcagcc acttttagcg
     2281 cagtggcaga aaaagccgaa gtgtattttt caaacatcgc gcgtttatcg agcaatatac
     2341 gcataaacag cgccgcgccg acggtattgg taaccatcat tggcgcagca atattactca
     2401 ccagacgcac cgcatcttca taaggtcggg cgatcgccag gatgatcaac atttgcacca
     2461 tttcagcgac gaacgtgacg gcaccggcgg taatggggtt aaagacttta tcagtgcgcc
     2521 cgcgacggat caggatgctg tgtaccaggc cgccgagcaa cccttcaacg atggtcgaga
     2581 tcatgcaact tagcgcggtc atgcccccca tcgaatatcg atgtaacccg ccggtcagac
     2641 caaccagccc accgacgacc ggaccgccga gtaagccgcc cattaccgcg cctatcgcac
     2701 gggtattggc aatagaatcg tcaatatgca gcccaaacca ggtgcccatg atgcagaaga
     2761 tggaaaagac gatgtagcag agaaatttat gcggcagacg aaccgtgacc tgcattaacg
     2821 gtatgaataa tggcgtttta ctcattaacc acgcaatgac taaaaaaacg cacatctgct
     2881 gaagcagcag caacaccaga ttaaaatcgt acatacccgc aaaccacact tccctttaaa
     2941 acgcgtaaca tacattgcct gcgtttaact ttctttgaac tcttgcagaa aaatgagaat
     3001 tcgtgagtac gatcactcaa aatcgcctgg caaaaataaa atcaccctat agatgcacaa
     3061 aaaacgggca aaactacctg gttcgcaaaa ctgcgtctaa agttaaaccg ggacctcgcg
     3121 agcaagggtg agacgatggc gctttacaca attggtgaag tggcgttgct ttgtgatatt
     3181 aaccctgtca cgttacgcgc gtggcagagg cgttacggat tgctgaaacc gcaacggaca
     3241 gacggcggtc atcggctgtt caatgatgcc gatattgacc ggatccgcga gatcaaacgc
     3301 tggatcgaca acggcgtgca ggtcagcaaa gttaaaatgc tgctcagtaa tgaaaatgtt
     3361 gatgtgcaga acggctggcg cgatcagcaa gaaacattac tgacttacct gcaaagcggc
     3421 aatctgcata gcctgcgaac gtggatcaaa gagcgcggtc aggattaccc cgcccagaca
     3481 ctcaccacgc atctgtttat tcctctgcgc cgacgacttc agtgccaaca accgactctc
     3541 caggcgctgc tggcgatcct cgacggcgta ctgatcaact acatcgccat ttgcctggct
     3601 tcggcacgta aaaaacaggg taaagatgcg ctggtggttg gctggaatat tcacgatacc
     3661 acccgtctgt ggctggaggg ctggattgcc agtcaacaag gatggcgcat tgatgtcctc
     3721 gcccactcgc tcaatcaact acgccctgaa ctgttcgaag gccgtacatt gctggtgtgg
     3781 tgcggtgaaa atcgaacctc cgcccaacag cagcaactca ccagttggca ggaacaaggc
     3841 catgatattt tcccactcgg catttaatga ttcgttaaca aatgcgcttt actgtacaat
     3901 cctttcgtta acataaggag tgcattatgc gcatagctaa aattggggtc atcgccctgt
     3961 tcctgtttat ggcgttaggc ggaattggtg gcgtcatgct cgcaggttat acctttattt
     4021 tgcgtgctgg ctaagcgcct gcaccagcct ttcaaacagg cggtctgcaa tgatcgccgc
     4081 cagtgccacc agtaacgccc cctggatcac atacgcggta ttaaatccgc taagcccgat
     4141 gatgatgggc gtacccagcg tgctggcccc taccgttgag gcgatcgtcg ccgtaccaat
     4201 gttgataatc accgaagttc gcacacccgc cagaatcacc ggagccgcca gcggtagctc
     4261 gaccttacgc agtcgctgac ctcgactcat tcccatactt ttcgcaactt ctgtcacgct
     4321 ggcatcaatc gctcccagcc cggcaagtgt cgcctgcagg acgggcagca caccataaag
     4381 gatcaaggcg ataatcgcgg gttgcagacc aaacccaatc accggaacgg caatcgccag
     4441 caccgcgacg ggcggaaaag tctgtccaac ggcggcaata gtttccacca gtgggcgaaa
     4501 ttccgcgccc cacgggcgag taacaataat tcctgcacca gtgccaatga tcaccgcaaa
     4561 caaactcgaa attcccacca gccagaaatg agccagtgcc agagcagcaa aactttcttg
     4621 ctgataaacg gggcgtggca gttgtgggaa caaggcagca aacagcggct ggctgtaagg
     4681 cagccagaaa atcagtgcca caaacaaagc aatgagccag aacagcggat cgcgcaacat
     4741 cttcatacgc ttacgcctcc accagcagat cctgaaaatg cagcgtgccg caaggctcgc
     4801 cctgcgtgtt caccaccggc agcacttcac atccccgcgc gacaaacagg gagagcgcat
     4861 cgcgtagcgt catctcttct gccagcgcct caccttctgc ccgttcttcg cgacgcacgt
     4921 aatccgccac gctacgtaac gaaagcaggc ggacacccag ttcactatgc ccaaaaaact
     4981 ggcggacaaa atcattcgcc ggacgagtca gcatcgtcag cggattgccc tgctgcacca
     5041 cttcaccgtg atccatcaac accagatgtt ctgccagtcg tagcgcctca tcaatatcat
     5101 gagtgaccag cacaatggta cgccccagca aacggtgtat gcgcgtcatc tcttgttgca
     5161 acgcgccgcg cgttaccggg tccagcgcgc caaaaggttc atccatcagt aagacttgcg
     5221 gatcggcagc cagtgcgcgc gccactccca cacgttgctg ctgaccaccg gaaagctgat
     5281 gcggataacg ctcacgcaaa tttggctcca gccccagtag cgccattaat tcgtcgatac
     5341 gatcgtcgat ccgcgcccgt gaccattttt gtaattgcgg cacggtggca atattttgcg
     5401 ccacgctcca gtggggaaac aggccaatag attgaatggc atagcccatc cggcggcgca
     5461 actccagcac tggcagcgag cgaatttctt ctccggcaaa gcggatcacg ccgctgtcat
     5521 gctccaccag gcggttaatc attttcaggg tggtggattt accggagcct gatgtaccaa
     5581 tcagcaccga aaaactcccc tcctgaaaat tgagattgag atcgttaacg gctttttgtg
     5641 cgccgaacag tttgctgaca tggctaaatt caatcattac gtttcacctt cagcagtgcg
     5701 ataagcaaat cgaacagcgc atcgatcagc accgccagaa caattaccgg gatcaccccc
     5761 agcaacacta aatcaatggc actgcttagc agcccctgga aaaccagcgc accaaaaccg
     5821 cctgcgccga ttaacgccgc aatcaccgcc atgccgacgg tttgcaccat caccacccgc
     5881 aggctacgca gaaataccgg taacgccagc ggtaactgaa catgcaggaa tcgctgcgcc
     5941 ccgctcatgc ccatcgctct ggcgctctcc agcacatcgc gcgggatctg gttcaagccg
     6001 actaccacgc cgcgcaccag cggcagcaag gcatagagca ccagcgcaat cagtgcgggt
     6061 gtcattccgg ttcctgatat gccgagctta cccagccacg gaaaggccgt aaccagcgcg
     6121 gcaagcggcg caatcaacag gccaaagagc gccaccgaag gcacggtctg aatgacattg
     6181 agtagcgaaa aaatcgctcc ctgccgcgca gtggaaaagt agcaccagac gcccaacggc
     6241 acaccaatca ctaacgcagg cagcaccgca ccaaacagca acgtcagatg ttgtgccagc
     6301 gcgtcgtcaa acacatcctg acggttggcg tattctttca ttagcgagag atcgttaagc
     6361 gtgccggagt acagcaacca cagcggaata atggcaatct gcatatgcaa caaccagcgc
     6421 cacagcagat gcgtggagat tcggcggatg gcatcgctac aggccagcaa tgccagcgcc
     6481 gcagccagcc agaaaccact gccgaggctg gtacgcgcca gcgcactgcc attttgtgcc
     6541 agttgtgtcg ccgcctttcc agctccccac accagcaata cgaagacgaa ttgcgccaga
     6601 atgagtgcac aaatgctgcc ttttttaccg ggaataacac aggcggtcag ccaggcgcaa
     6661 ccaacgccca ccagcatcca gatcgtttgc ggccacagct gccagagatg acgcccctca
     6721 cccgaaacta aacgattagg cgcgtaactg ataaacggca gcgccgctgc gattgccgtc
     6781 agcaacagca gcagcgccag aacaggatta atacgtagat aagtcacggg aaattacttc
     6841 gtccaccctt tttgtttcag gtagtcggca gccacttttt tggcatccag tccttcaact
     6901 gcaatgctgg cattcagttg ctgcaatgtt tttgcatcga ggctggcgaa gactggctgt
     6961 agccactgtg ccatttgcgg atactccttc aacaccgatt cacgaaccac tggtgcaggc
     7021 gcgtagatag gttgcacgcc ttgcggatcg cttaaggttt gcaaccccag cgctgcgacc
     7081 ggaccgtcag tgccgtaagc cattgcggca ttaacgccag aagtttgctg ggcagcggct
     7141 ttgatcgtta ccgccgtgtc gccaccagcc agtgacagca actgatcctg atcgagttta
     7201 aagtcgtagg ctttttcaaa agcgggtaag gcatcggcgc gttcaataaa ttctgccgag
     7261 gccgccagtt tgaaggtgcc gccctctttc agataacgac tcaggtcggc aagcgaagtg
     7321 agtttgtttt tctctgccac atcctgacgc acggcgatag tccaggtgtt atttgcaggc
     7381 gcgggcgtca gccagattaa cttgttttgc tctgcatcga gttttttgac tttctcgtag
     7441 ccttgcccgg catttttcca tgccgcatcg ttttcatctt taaagaaaaa cgcgccattg
     7501 ccggtatatt ccggatagat atccagttca ccggaagtaa tcgccccgcg caccacggga
     7561 gtcgttccca gttgcacttt atttaccgta ggaacaccgt gactttccag cacctgcaaa
     7621 atgatattgc cgagcagcgc gccttcggta tcgatttttg aaccgacctt aacgggggaa
     7681 gccgcctgta gcggcaggct cacggctgcc aacaaaacca atgaacctgc ccagagcttt
     7741 gagagtggca tgatgctttc ctcattcttt tactgttgtt ttcagcgaat taagagaaaa
     7801 gcatagttga taatgacagg gttagcctgt ctgggcggat tcagttgcag aatcagataa
     7861 atacttaaag ggcgcagacg tcgcgccctt actaaagcat tacagcaact caaactcgcc
     7921 tttcttaacg cgtgcggaat cagtgccgat aaagacattg aacttgccag gctcggcgtc
     7981 atatttcatc tgttgattcc agaacttcag cgcctcgata tcaatcggga agctgacggt
     8041 ctgggtttcg cctggtttca gggtgatttt ctcaaagcct ttcagctgct tcaccggacg
     8101 actcatggaa gccgtcacat cctgcaagta catctgcact accgtcgccc cttcgcgctt
     8161 accggtgttc gtcacctgca cactggcggt cactttgccg tcacgcttca tggtcggcgc
     8221 ggaaagtttc acatcagaga cggtgaaagt ggtgtagctc agaccatagc cgaacggata
     8281 cagcgccccg ttagcttcat caaaataacg cgacgtgtat ttgttcggct tatcggcgtt
     8341 atacgggcga ccggtattca gatggctgta gtacaccggg atctgcccga cagaacgcgg
     8401 gaaggacatc ggcagcttgc cggacgggtt gtaatcacca aacaatacat cggcaattgc
     8461 attaccgcct tcagtccccg caaaccaggt ttccagaatc gcatccgcct gctgatcttc
     8521 tttcaccagc gccagcggac gcccgttcat cagcaccagc accagcggtt tagcggtggc
     8581 tttcagcgcc gcaatcaagt cacgttggct ttgcggaata gtgatatcgg tacggctgga
     8641 agcctcgtgc gccatcccct gtgcttcacc gactacagcc accaccacat cagattgttt
     8701 cgcggtctgc accgcttcat caatcatctc ttgcggcgaa cgcggatcga ctttgaccgc
     8761 ttcttcatac tgattcagga aatcgataat gcctttgtca ctggtaacgt tcgccccttt
     8821 ggcatacagc actttaccgt tttcaccgac ggcgttttta atccctgtta gcacagtaac
     8881 agattgatcg gcgacaccgg ctgcggacca gcttcccatc acgtcacgct tgctgtctgc
     8941 cagcgggcca accaccgcaa tggtggctga ttttttcagc ggtaacgttt cgagacggtt
     9001 tttcagcaac accaggcttt cgcgtgccac ttcacgcgct tctttgcggt gcaggcggct
     9061 ttcggcattg gtatccaccg ggtcagactc tttcggcccc aaatggctgt atgggtcgtt
     9121 aaacaacccc atatcatatt taacgttcag tacatgacgg gcagcgtcat ccagctcttc
     9181 catcgtcact ttgccggatt tgatcaaccc aggcagatac ttcgagtaat actcgtcgct
     9241 catactcatg ttgatgccgg atttcagcgc cacgcgcacc gcatcttccg ggtctgccgc
     9301 cgtgccatgt ttaatcagct ctttgattgc gccgtgatcg gaaacggtga tgcctttaaa
     9361 gccccactgg tcgcgcagaa catctttcag cagccaggag tcggaggtgg ctggcgtacc
     9421 gttcagcgag ttcagcgcca ccatcaccgc gccgctgcct gcgtccagcc ccgctttgta
     9481 cggcggcata taatcattaa acaggcgctg cggactcata tcgacggtgt tgtactcttt
     9541 accgccttct accgcgccgt atgcggcaaa gtgtttgacg ctggtcatca ccgagtaacg
     9601 atctgccggg cttttaccct gcatcgcttc caccatggtt ttacccattg ttgaagtgag
     9661 atacgtatct tcgccaaaac cttcggaagc acgtccccag cgcggatcgc gcgagacatc
     9721 gaccatcggt gcccaggtca tattcaggcc atcatctgcc gcttcataag cagagacacg
     9781 tccgaccgtt ttcaccgcat cgaggttaaa agacgaggcc agaccgaggc taatcgggaa
     9841 caccgtgcgc tgaccgtgca gcacgtcgta agcaaagaaa agaggaattt tcaggcggct
     9901 taattccatc acctgatcct gcatggcgcg gatatcctga cgggttacgg tgttgaaaat
     9961 cgccccaacc tgcccgtctt tgatcatctc gcggatcgcc tctttcggat tatccgggcc
    10021 gacgctgatt aagcgcagct gaccaatttt ctcatcaacc gtcattttct taagcagttc
    10081 ggtgacgaac gcatcccgcg cttcaggcgt taagggatgg ttaccgaata aatcatccgc
    10141 cagtgctggc tgcagggcca gacttaccgc gattcctact gaacatagcc atttcatatg
    10201 gatttttctt cctttgttgc ccgacgtggc agcgaaaatg gtgcaaaaac cgtagtttgc
    10261 cataagcatg atggagagag aaaaagaatg ctcagtttat tgtctgaatt ttcaaaatat
    10321 tcactcgctg aattgtcata caaggcgcta ttctagtttg tgatattttt tcgccaccac
    10381 aaggagtgga aaatgtcttc catgacaaca actgataata aagccttttt gaatgaactt
    10441 gcccgtctgg tcggtcattc acacctgctc accgatcccg caaaaacggc ccgctatcgc
    10501 aagggcttcc gttctggtca gggcgacgca ctggctgtcg ttttccctgg ctcactactg
    10561 gaattgtggc gggtgctgaa agcctgcgtc accgccgaca aaattattct gatgcaggct
    10621 gccaatacag gcctgaccga aggatcgacg ccaaacggta acgactatga tcgcgatatc
    10681 gtgatcatca gcaccctgcg tctcgacaag ctgcacgttc tcggcaaggg cgaacaggtg
    10741 ctggcctatc cgggcaccac gctttattcg ctggaaaaag ccctcaaacc gctgggacgc
    10801 gaaccgcact cggtgattgg atcatcgtgt ataggcgcat cggtcatcgg cggtatttgt
    10861 aacaactccg gcggctcgct ggtgcaacgt ggcccggcgt ataccgaaat gtcgttattc
    10921 gcgcgtataa atgaagatgg caagctgacg ctggttaacc atctggggat tgatctgggc
    10981 gaaacgccgg aacagatcct tagcaagctg gatgacgatc gcatcaaaga tgacgatgtg
    11041 cgccacgatg gtcgtcacgc ccacgattat gactatgtcc accgcgttcg tgatattgaa
    11101 gccgacacgc ccgcacgtta taacgccgat ccggatcggt tatttgaatc ttctggttgc
    11161 gccgggaagc tggccgtctt tgcggtacgt cttgatacct tcgaagcgga aaaaaatcag
    11221 caggtgtttt atatcggcac caaccagccg gaagtactga ccgaaatccg ccgtcatatt
    11281 ctggctaatt tcgaaaatct gccggttgcc ggggaatata tgcaccggga tatctacgat
    11341 atagcggaaa aatacggcaa agacaccttc ctgatgattg ataagttagg caccgacaag
    11401 atgccgttct tctttaatct caaggggcgc accgatgcga tgctggagaa agtgaaattc
    11461 ttccgtccgc attttaccga ccgtgcgatg caaaaattcg gtcacctgtt ccccagccat
    11521 ttaccgccgc gcatgaaaaa ctggcgcgat aaatacgaac atcatctgct gttaaaaatg
    11581 gcgggcgatg gcgtcggtga agccaaatcg tggctggtgg attatttcaa acaggccgaa
    11641 ggcgatttct ttgtctgtac gccggaggaa ggcagcaaag cgtttttaca ccgtttcgcc
    11701 gctgcgggcg cagcaattcg ttatcaggcg gtgcattccg atgaagtcga agacattctg
    11761 gcactggata tcgctctgcg gcgtaacgac accgagtggt atgagcattt accgccggag
    11821 atcgacagcc agctggtgca caagctctat tacggccatt ttatgtgcta tgtcttccat
    11881 caggattaca tcgttaaaaa aggcgtggat gtgcatgcgt tgaaagaaca gatgctggaa
    11941 ctgctacagc agcgcggcgc gcaataccct gccgagcata acgtcggtca tttgtataaa
    12001 gcaccggaaa cgttgcagaa gttttatcgc gagaacgatc cgaccaacag tatgaatccg
    12061 ggggttggta aaaccagtaa gcggaaaaac tggcaggaag tggagtaaaa attacggatg
    12121 gcagagtatc gccatccgtc attcatttaa tcgttctgtg ccgtctgccc cgccgccgcc
    12181 atttgggcgg ctttttgttt tttatagctc aacgctgctg ccggaacagg catcacttta
    12241 ccggtctcaa tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac
    12301 gcgtccatca ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa
    12361 tggcccgccg cattggtaaa gcccgttttg gttaactgaa tattccagtt atcgcgatac
    12421 accagatggt tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata
    12481 tcttccctgg tagtacttaa ctgaccaatc aacggatatt gtttgctggc aatgagcagt
    12541 ttggttaagt cacgggcagt tgaaacgtta tgcaccgaca atccggtcgg ttcaacaaag
    12601 cgcgtgttgt tcattccgag cgatttcgct ttcgcattca tcgccttaat aaaggctttg
    12661 taaccaccgg gataatggtg cgcaaggctt gccgccgcgc ggttttctga agacatcagc
    12721 gccagcaaca gcatatcttt acggctgatt tcgctgttca gtcgtacgcg cgaatagacc
    12781 cctttcatct ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt
    12841 gcatccagca caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga
    12901 tccgggtggt tcgaatagat cactttgttg gtattcagat caacaatcat cgcgctaccg
    12961 gaggcaattt ccggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt
    13021 gccaaaggca cagccagcat cagggccagg ctaaataaag aaactcgaaa tttcggcatg
    13081 atgagcattc agatagtggt tcacgcgcac gggttgcgca ccgccggagt aaggatttac
    13141 tgaggctagc gacgccatca taacgagcaa aaagtgcgat cgtcaaagaa gaatcgtgag
    13201 gaaatgctgc attgctgaca tttacgccag caatgcagcg caaaagaact ttctagaaca
    13261 aacgataacc gtagccccat agtataacgg taagggcgag cagcacttcc agcaccagca
    13321 cgcctatcgc cagtgtcgaa ctggagaagc taagtccttc ctctttgttg atattcagga
    13381 agctcggaat accgagatac agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca
    13441 gcgcacacaa ccagaccagc gggtaaagcg ccaccagacc gcttaaaaac agcggagttg
    13501 caacgtagcc cgcgaagacc atgcagtgcg caagtgacgg acgctgcgga taattacgcg
    13561 ccatccacca gatgacccgc cccatcaccg caaccccagc cagcataacg ccgtaaaaca
    13621 agacagccag cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat
    13681 tccagccaat ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca
    13741 aaacgtggtg ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt
    13801 cacgatcggg atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt
    13861 tcatgaacct gacagttcaa gtataagtca gcttgtgatt attttttgtt cgctcatgta
    13921 atttgcaccg tctgatgtac gtcctttttc agcgtgaact attcacaggg tgtatgctta
    13981 caggcaagca aagggagaca gactggccta tggatctcaa tacactcatc tcacaatatg
    14041 gttatgccgc gctggtgatc ggtagcctgg cggaaggtga aaccgtgact ttgctgggag
    14101 gcgttgcggc acatcagggg ctattaaagt tcccgctggt ggtactttct gtggcgcttg
    14161 gcggcatgat tggcgaccag gtgctttatc tgtgcgggcg gcgctttggc ggcaagctat
    14221 tacgccgttt ctcgaaacat caggataaaa ttgagcgggc gcagaaactt atccaacgcc
    14281 atccgtatct gtttgtcatt ggtacgcgtt ttatgtatgg ctttagggtg attggcccga
    14341 cgctgattgg tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg
    14401 catttgcctg ggcgttgatt tttaccacta tcggttatgc tggtggtcag gtgattgcgc
    14461 cgtggttgca caatctcgac cagcatttga agcactgggt ctggttgatt ctggccgtgg
    14521 ttctggtggt aggcgtgcgc tggtggctga aacgacgcgg gaagaaaaag ccggataatc
    14581 aggcgtaaag cagttgccac agataagcat tcacgccaca tccggcaccg gatgttccat
    14641 gcctgatgcg acgctggcgt gtcttatctg gcctaaccca ttgaaataac cacgcacctc
    14701 actgcgctat tctgggttga actgcggatt cgccaacata aagccgccat ccactatcaa
    14761 cgactgcccg gtggtgtaat ttgcgccctc cgaacaaagc cataccacca ggctggcaat
    14821 ctcatgcgtt gtgccaaaac gtcgcaaggg aatcgaaggc tccgcgtcgg gcctcacatc
    14881 gctatcatcc atgccgttca ttggcgtggc gatcgcccca ggtgcgactg cgttcaccaa
    14941 aattttatgc ctgaccaact ccagcgccat tgctttggtt aacccgccga gcgcatgttt
    15001 agctgctgtg taagcgctgg catccggcag cggcgtatgt tcatgcaccg acgtaatgtt
    15061 gatgatgcga ccgccctgcc cttgtttcac catctgacga gccgcaattt gcgagcataa
    15121 gaatgcaccg tcgacatcaa cggtaaaaat cttgcgccac tcatcaaaag ccatatcaag
    15181 aaacggcgct ttggtcattg cacccgcatt attcaccagc acatcgatgc gccccagtcg
    15241 ttgaatgagt ttctccagcg cctgcgcacc ttctggcagt ttaccgagat ccagctgcac
    15301 gatctccgca cgtgcgccgt ggctaaccac ctcacgcgca gtatcttttg ccccttcttc
    15361 atcagagtgc caggtaatgc caatatcaaa cccctgctgc gccagtaata acgcgcactc
    15421 tttgccgatc cccgaatcgg aggcggtaat aatcgcaacc tgtgccatcg agttctccac
    15481 ttaacgctga ataaacgtta agtatagaag gcgcatatca tcagcgtttg taacccccgc
    15541 ccaacgcacc agtgagttga atggaggcat ccagccactg cccttgcaat aacaggccat
    15601 tggcccgctc gcgaagcgcc gggattctgg cttcgctgac gcgggaaccg gcaatgatgc
    15661 ccgcgttaaa gcgcgcctgt gcaagaccta ctacacgcaa ggcatcgcgc tcaatttgcg
    15721 cctgatgctg gtttttctcc gccagtgtct gaacctgact ggctgcccgc gccacgtcat
    15781 tcaccgcgtc aaccaccgct ttgttgtagc tggcgataga caagttgctt tcggcttttg
    15841 cgatatcgag gttggcgtta agacgaccac tatcgaaaat gggtagcgtc aggcctgccg
    15901 taacgcccat ttgctgcgcg gaatgacgga acagatcgct taagtgcaac gcatcctgtt
    15961 gcaggaaggc catcaggttg atgtcaggat aaaatgccgc ttttgccgca tcaatagtgc
    16021 ttaacgatga ctcaacgtac cagtgcgccg cctgcaaatc tgcccgccgg gccagtaagg
    16081 agtaccccag ttcatcagga agctggcttg ccactttcgg caacgcgacc ggattaagct
    16141 tcaatgcctt tgtctggtga tttgtaagtg cacttaaccg tgcctcaata attttcattt
    16201 tccccgcgac atcgttgagc tgctgccggg ttttgctggc attaatatcg gtttccacac
    16261 cttcaactga agaagtaatc ccgttctgat atagctggcg atcggtggcg ataatggtgt
    16321 tctgctcttt ttcgatttgc tgcaagaccg tgtttaacgc cgcctgggtt tgccactccc
    16381 agtacaggcg ggctacgctg ccagccagca attggcgggt ttgctcgcgt tccgccgctc
    16441 gtgctttaac cgtacccagg cgagcagtta cctccgcccg attctttccc cagatatcga
    16501 gatgccagcc cgccgttaag ccaaaagtac cgttggtgta ccacggaccg gtcgtacctg
    16561 cggccggatc gttcagagca aacggcccca ttaagccttc tgccgacatt ttttgccgct
    16621 ccatatccgc agaaaagtcg agctgcggac catcctgagt agcaattgcc ttcgcctggg
    16681 cttcagccag ctgaatgcgc tgttcagcca cctgcataac cggtgcgttc tgtagtgcgt
    16741 tattaattaa tgaggtgagt tgattatcgt gatactccag ccaccattgg ctgtctggcc
    16801 aaccattttt cagcgccgtg ggtaatgcgg tgtcaacttg cacagcgggc gtgtgctggc
    16861 ttaacgcctg gcgggtttca tgcataggcg cacacccggc cagcatcagt aacagcggaa
    16921 aacaggcgat ggctggataa aaggaatcac gattcatggg ggaataatca ggtaagaaaa
    16981 ggtgcgcgga gattaccgtg tgttgcgata tattttttag tttcgcgtgg caatacatca
    17041 gtggcaataa aacgacatat ccagaaaaat atacaccaag tgaatgatat cttccgattt
    17101 atcttaatcg tttatggata gcggcaaagg gcttcgtttt ttcctatact tattcagcac
    17161 tcacaaataa aggaacgcca atgaaaatta tactctgggc tgtattgatt attttcctga
    17221 ttgggctact ggtggtgact ggcgtattta agatgatatt ttaaaattaa ttaatgtcat
    17281 cagatccgaa aataacgaga atatttcagt ctctcatcct gttgcgctcc tgtcatgtgc
    17341 attgcttcat ataatcactg gcgca
//
LOCUS       DAZKGF010000102        17307 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZKGF010000102 DAZKGF010000000
VERSION     DAZKGF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.102"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(51..782)
                     /gene="rlmB"
                     /locus_tag="ACHOQV_003206"
     CDS             complement(51..782)
                     /gene="rlmB"
                     /locus_tag="ACHOQV_003206"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP9631536.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(873..3314)
                     /gene="rnr"
                     /locus_tag="ACHOQV_003207"
     CDS             complement(873..3314)
                     /gene="rnr"
                     /locus_tag="ACHOQV_003207"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP9631537.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3353..3778)
                     /gene="nsrR"
                     /locus_tag="ACHOQV_003208"
     CDS             complement(3353..3778)
                     /gene="nsrR"
                     /locus_tag="ACHOQV_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFP9631538.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3983..5281)
                     /gene="purA"
                     /locus_tag="ACHOQV_003209"
     CDS             complement(3983..5281)
                     /gene="purA"
                     /locus_tag="ACHOQV_003209"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP9631539.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5385..5582)
                     /gene="yjeT"
                     /locus_tag="ACHOQV_003210"
     CDS             complement(5385..5582)
                     /gene="yjeT"
                     /locus_tag="ACHOQV_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFP9631540.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5664..6668)
                     /gene="hflC"
                     /locus_tag="ACHOQV_003211"
     CDS             complement(5664..6668)
                     /gene="hflC"
                     /locus_tag="ACHOQV_003211"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFP9631541.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6671..7930)
                     /gene="hflK"
                     /locus_tag="ACHOQV_003212"
     CDS             complement(6671..7930)
                     /gene="hflK"
                     /locus_tag="ACHOQV_003212"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFP9631542.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8016..9296)
                     /gene="hflX"
                     /locus_tag="ACHOQV_003213"
     CDS             complement(8016..9296)
                     /gene="hflX"
                     /locus_tag="ACHOQV_003213"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFP9631543.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGEGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9373..9681)
                     /gene="hfq"
                     /locus_tag="ACHOQV_003214"
     CDS             complement(9373..9681)
                     /gene="hfq"
                     /locus_tag="ACHOQV_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFP9631544.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9767..10717)
                     /gene="miaA"
                     /locus_tag="ACHOQV_003215"
     CDS             complement(9767..10717)
                     /gene="miaA"
                     /locus_tag="ACHOQV_003215"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP9631545.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10710..12557)
                     /gene="mutL"
                     /locus_tag="ACHOQV_003216"
     CDS             complement(10710..12557)
                     /gene="mutL"
                     /locus_tag="ACHOQV_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP9631546.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTVEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKMVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPLYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12567..13904)
                     /gene="amiB"
                     /locus_tag="ACHOQV_003217"
     CDS             complement(12567..13904)
                     /gene="amiB"
                     /locus_tag="ACHOQV_003217"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFP9631547.1"
                     /translation="MMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPSPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTFLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13923..14384)
                     /gene="tsaE"
                     /locus_tag="ACHOQV_003218"
     CDS             complement(13923..14384)
                     /gene="tsaE"
                     /locus_tag="ACHOQV_003218"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP9631548.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14356..15903)
                     /gene="nnr"
                     /locus_tag="ACHOQV_003219"
     CDS             complement(14356..15903)
                     /gene="nnr"
                     /locus_tag="ACHOQV_003219"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFP9631549.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15902..17041
                     /gene="queG"
                     /locus_tag="ACHOQV_003220"
     CDS             15902..17041
                     /gene="queG"
                     /locus_tag="ACHOQV_003220"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP9631550.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17024..17077)
                     /locus_tag="ACHOQV_003221"
     CDS             complement(17024..17077)
                     /locus_tag="ACHOQV_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631551.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3923 a         4943 c         4345 g         4096 t
ORIGIN      
        1 gacaaaaaaa aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc
       61 tgacgaaccg cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca
      121 cttcctgcca ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata
      181 ccttcacctt ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt
      241 gtatgatccg cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc
      301 atggtgcgcg ccagattagt cacccgaatc agcggaacgc tttctgccgc accacaggcc
      361 acttttttcg ccgtggcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcatga
      421 acaccagcgg cgtccgcgct acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg
      481 tcgaggatca gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc
      541 tgatactgcc gtcctggctt cacacgagcg ataatgccct gatgcacggc accgtcgctt
      601 ttctcgtcga gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg
      661 tgaatcagcg gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa
      721 cgttcagggg cgcgctccag cagggcctgc actgcatgga tgccgtaaat catttcgctc
      781 attaatgtac tcgttattta cgttttgccg ggagcgccac gcttgcccgg cctacggata
      841 attcaccctc ttctgtttga agagggtaat gatcactctg ccactttttt cttcgccgca
      901 cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg ccgatggctt tttcgctttt
      961 ctcgcgtctt tcttcgctgc tttcggcttc ggttttttct cgccgcggaa ggcgctgtct
     1021 ggctcaaagt tgaccttttt gccgacctga cgccgcttgc cgcctttttt gcctgcatcg
     1081 ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc gcggtgcgcg ttcgctggag
     1141 atcaggctaa agtcgatttt gcgctcgtcc atattaaccg cttcgacgcg aacttccacg
     1201 cgatcgccca ggcgataagt ctggccgctg gattcgccca tcaggcgttg ccccacctgg
     1261 tcaaagcgat agtagtcatt gtccagcgaa gagacatgga ccagaccatc aatgaacaag
     1321 tcatccaggc gaacaaagaa gccaaaacca gtgacgctgg aaattacgcc tttaaagacg
     1381 ttacctacct ggtcgagcat gaagtcacac ttcagccagt cagccacatc gcgcgttgct
     1441 tcgtcggcac gacgttccgc catcgaacag tgctgaccca gttgcagcat ctcttccatc
     1501 gaataatggt agccgccggt ttcagtggta ttcccctgat gcccctgctc tttcgccaac
     1561 agatacttaa tggcgcggtg cagcgtcagg tcaggataac gacgaatcgg cgaagtaaag
     1621 tgcgcatagg actgcaatgc cagaccgaag tgaccacggt tttccggatc gtaaatcgct
     1681 tgtttcatcg agcgcagcag catggtttgc agcatttctg catcaggacg gtcggcaacc
     1741 gactccagca gctctgcgta gtcgcgcggt tccggcttgt taccgcccgg cagttccagc
     1801 cccagctccg ccagcactga acggaaagag gtaatcgctt cggtgctcgg cttgtcgtga
     1861 atacggaaca gtgccggttc tttcgctttc tcaacgaaac gcgccgccga gatattcgcc
     1921 agaatcatgc actcttcaat taacttgtgt gcgtcgttac gctgagtctg ttcgatacgc
     1981 tcaatacggc gttcggcgtt gaaaatgaac ttcgcttctt cgctctcaaa tgagatcccg
     2041 ccacgttctt cacgggcttt atccagcact ttatagaggt tatgcaactc ttcgagatgc
     2101 ttaaccagcg gggcatactg ctcgcgcaga tcctgatcgc cttgcagtat atgccagact
     2161 ttggtgtagg tcagacgcgc gtgagaactc atcaccgctt cgtagaattt gtagccagtc
     2221 aggcggcctt tcgacgaaac cgtcatctcg cacaccatac acaggcggtc tacctgcggg
     2281 ttgagcgagc acaggccgtt agagagcact tccggcagca tcgggataac ctgcgaaggg
     2341 aagtacaccg acgtaccacg gttacgcgct tctctgtcca gcggtgttgg cggacgcaca
     2401 tagtaactga cgtcggcaat tgcgacccat aaacgccagc cgccgccgcg ttttttctca
     2461 cagtaaactg catcgtcaaa gtcacgggcg tcttcgccat caatggtgac cagcggtaaa
     2521 tcgcgcagat cgacacggcc cgcttttgct tcttccggca cttcttcttt cagcccggca
     2581 acctgttgct caacagcctg cggccagatg tacggaattt catgggtacg cagagcaata
     2641 tcaaccgcca tgccggtgcc catattatcg cccagcactt cgacgatttt acccaccgct
     2701 ttggtacggc gagtcgggcg ttgagtcagt tcaaccacca ccacaaagcc catccgcgcg
     2761 cccatgatct gatcgggcgg gattaagata tcgaagctca gacggctatc gtcaggaacc
     2821 acaaagccga cgcccgcttc ggtgaagtag cgaccaacaa tctggctggt ttttggcacc
     2881 agtacgcgga caatacgcgc ttcacgacga cctttacggt cagcgcccag cggctgagcc
     2941 agcacctgat cgccatgaat gcaggttttc atctgctcgc tggagagata caaatcatct
     3001 ttacgccctt caactcgcag aaagccgtag ccatcacggt ggccaataac ggtacctttc
     3061 accaggtcga ggcgttccgg cagcgcatag cactgacggc gagtgaagac cagttgacca
     3121 tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa gctgctcttc gccttcaatg
     3181 tgcagttcta ccgccagctc atcacggctg gccggttttt cacgtttggt taaatgttcg
     3241 aggataaatt cccgactagg gatgggattc gcgtattttt cagcttcgcg ttcctggaaa
     3301 ggatcttgtg acatctcggt tcctccgttg tcatctctga tgaagatttt cgtcactcca
     3361 ccagcaataa tttataaagc ggttgattct cttcaaccaa atcggcaagc gtgtagttat
     3421 ccagttccgt aagaaaactt tgcacggcct tagaaagcgc ctgtttcaac cgacaggcag
     3481 gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga taagggctcc agctcgcgca
     3541 ccacatcacc aatacgtatt gcactcgccg gtttacccaa gcgaatgccg ccattttttc
     3601 cacgaacagc agtcacgtag ccggcacgac taagttgatt gattattttg accatatgat
     3661 tacgggagac gccgtagacg tcagtcactt cagaaatact ggtcatccgc ccttctggca
     3721 atgacgccat gtagatcagc gcacgtaatc cgtaatcagt gaaactcgtt aactgcacat
     3781 caacctcaaa agggaaatcg gggaaaaata catttatatt gatgataaac cagccacaag
     3841 ctgtgtcgct aatttatttc agttcgggga aggaaaaaag cgaggattta acactgtgtc
     3901 ggatagcgta atggcttaac cgacacagca atgtatcaca ctgttagccc ggcaggcaaa
     3961 atatctgcca ggcgtaccag aattacgcgt cgaacgggtc gcgcagaatc atggtttcag
     4021 tacgatccgg accagtagag atgatatcga tcggcacacc ggtcagctct tcaatgcgct
     4081 tgatgtagtt cagtgccgcc tgcggcaggc cgctacgatc tttcacgccg aaggtggatt
     4141 cagaccagcc cggcatggtt tcgtaaatcg gctctacacc tttccagtcg tcagctgcca
     4201 gcggagtggt agtcacttca cgaccatccg gcatacggta agccacgcag agtttaacct
     4261 ctttcaggcc atccagaacg tccagtttag tcaggcagaa gccagacaga gagttcagct
     4321 gtaccgcacg acgaacggca acggtgtcca gccagccggt acgacgacga cgacccgtag
     4381 ttgcgccgaa ttcgttaccc tgcttgcaga ggaactcgcc agtttcatca aacagttcgg
     4441 tcgggaacgg acctgcaccc acacgagtgg agtaagcttt gaggataccc agaacgtaat
     4501 caacataacg cgggcccagg ccggaaccgg tcgccacgcc accagcagtg gtgttggaag
     4561 aagttacgta cggatatgta ccgtggtcga tatccagcag cgtaccctgc gcaccttcaa
     4621 acatgacgaa atcgccacgc tgacgcgcct ggtcaagcag gtcagaaacg tcaacaacca
     4681 tagaagtcag gatgtcggca acagccatcg tatcatccag aactttctgg taatcaaccg
     4741 cttcagcttt gtagtagtta accaactgga agttgtgata ttccatcact tctttcagtt
     4801 tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg cagaccgcga cgtgccactt
     4861 tatcttcata agcaggcccg atacctcgac cggtggtgcc gatcgctttc gcgccacgcg
     4921 ctttctcacg cgcgttatcc agcgcaacgt gataatcaag gatcagcgga catgcttcgg
     4981 acagcagcag acgctcacga acggggatgc cacggtcttc cagttctttc atctctttca
     5041 tcagcgcagc cggagacagc acaacaccgt taccgatgat gctggttaca ttctcgcgga
     5101 gaatacctga tggaataaga tggagaacgg ttttttcacc gttgattacg agagtatggc
     5161 ctgcgttgtg accgccctgg tagcgtacaa catatttagc ccgttcagtc agaagatcga
     5221 cgatcttacc tttaccttcg tcaccccatt gggtgcccag tacgacgacg ttgttaccca
     5281 tttttcaaaa tcaccgtttg cttaaaaatg gattctacca tcgctttttc agagttacag
     5341 cactttttgc actcaaaaat gacctcgatc agtctgtttt ttgttcagcc aatcgttttc
     5401 ctcaacatgt agtagaccac aacgcccgca accacaagtc caccgccaaa acgacgtaaa
     5461 atattatcgg gcaaattggt catcgcagag atcatcttct tccatgcctt cgggtaaagc
     5521 atcggcccta aaccttccag taccaaaacc agggcaagcg ccagccagat tgtcgaattc
     5581 attcattatc cttatagaaa aagaaaacca ccgacatccc tgaggatgcg gtggctttat
     5641 tgacctgtac cgcagtcgtt atattaacgc gttgcggaag tcggcgtctt catgtagcgg
     5701 aagaaatcgc tatccgggct catgaccatc acgtcctgat tgccagagaa gctgttctca
     5761 taagcacgca ggctacggat aaatgcgtag aagtccggat ctttactgaa tgcatcagca
     5821 aacagtttgg ctgcttctgc atcgccttca ccacgcatga tgcggccctg acgctcagct
     5881 tcagccagcg ttctggtcac ttcatagtcg gcagtcgcgc gcagtttttc cgcttcttcc
     5941 tgaccttgtg aacggtgacg acgcgctacc gcttcacgct cggcgcgcat acggttgtag
     6001 atcgcttcag acacttcggt cggcaggttg atctgcttaa tacgcacatc gacaacttca
     6061 atacccagcg ccgccatact gttcgggttg atgaccggaa ctttgccctt cgtctcagcc
     6121 gttacgcgct ctgccgcttc ggcaatggcg ttatctgctg ccggggtagt aacttcatct
     6181 tctgtacccg cagaaccgga gttcagcgcg tcacgtactt cgagggtcag acgaccacgg
     6241 gaatcggtga cgatgtcttt cacgtccagg cgaccaattt cagaacgcag acggtcagag
     6301 aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc caccacccgt tgccaggtag
     6361 taacggctga aatcgctgat gcgccatttg atgtaagagt cgacgatcag gtctttcttc
     6421 tctttggtca caaagcggtc ggcctggttg tccatggtct gaatacgtgc gtcgagcatt
     6481 ttcaccgttt caatgaacgg tatcttgaaa tgcagacccg gctcataaac cagaggtttg
     6541 ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa taccgcgctc accttctttg
     6601 acgacaaaga cagacatgta aagcactacc agcacgatga tgataatcgc gataactgac
     6661 ttacgcatcg ttattccccc tgacgctggt agtcgttacg ctgcgcgttg gcgcggcgtt
     6721 ggtccataat atcgccctga ctggtggacg acgtgttgct tgctccactg gttgtggagg
     6781 aagaggctgg cggcagacgc agcagattgc tggcaccgtt atcgctcttc gccgcagggg
     6841 cgttaccacc tttcagcatc tggtctaacg gcagaaccat caagttgcca cctttatcgt
     6901 taaccagcac tttgcgggtg ttgcccaaca ctttttccat cgtctcgata tacagacgct
     6961 cgcgagtaat ttccggcgcg gctttatatt ccggcagaag tttagcaaag cgcgccactt
     7021 caccctgagc ttccaggatg gtctgggcct tgtacgcacg cgcctcttcg aggatacgtt
     7081 gcgcctgacc gttcgcacgc ggctgaactt cgttggtata cgcttctgct tcacgaatgt
     7141 attgctgttc gttttcacgc gcggcaatcg catcgtcaaa cgccgctttt acttcttccg
     7201 gcggacgagc tgcctggaag ttgacgtcca gcagcgtgat acccatgtca tacggacgaa
     7261 tcgtctcttc cagttcgcgc tgagtatcgc tacgaatcac ggtacgacct tccgtcagaa
     7321 tgcggtccat ggtgtattta ccgataaccc cacgcagggc gctgtcggta gcctgacgca
     7381 ggctgtcatc cgggctggtc acgctataca gatatttttc cggatcggtg acgcggtact
     7441 gcacgttcat ctcaacgcgc actacgttct cgtccgacgt cagcatcaca ccagaagcgg
     7501 ccagttcacg cacggcttcc acgttcaccg gtttgacttc gtcgataaac gtcggtttcc
     7561 agttcagacc cggctcaacc agatggctga atttaccaaa gcgtgttacc acgccacgtt
     7621 cggcttcttt aatggtatag aaaccactgg ccgcccagat aatgacaatc gctgccgctg
     7681 cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc ttgcgatgaa ctgccaccgc
     7741 cagatccggt gcctttaccg ccgcccagac caccgagctt tttgctcagt ttgcggaaga
     7801 tatcatctaa atcaggtggc ccttgatcgc gaccgccttt gtttccattt ccctcagagt
     7861 tgccgccagg tttgctgctt ccccacgggt cgcggtcttg tccgttatta ccgggctgat
     7921 tccacgccat gtttgtgctc catatttgtt atgcggtgat ccctgttgtc tttcgacgcc
     7981 agaggatatg actccacgct tcagacgcta cgccgttaga tcaggtaatc gatcaacgcc
     8041 ggttcttgtt tacagaggcg acgccagtca acgatcggca tacgaacttg cagacttacg
     8101 ctgccgtcct cctccatcca ctctttttct attgcctgaa gctgataaaa acgacttctc
     8161 agacgccctt cctgcggtgg cagacgcaat gtatgctgcg ccacctcgcc ggaaagccgc
     8221 tccgtcaaag cctgaaaaag ctgtggtatc ccctctccgg tctgtgcgga aagccagaca
     8281 cggatcggtt tgttctcttc gtcccgatca atacgcggtt cgaaatcttc cagcatatcg
     8341 atcttgttca tcaccagcag ggttgggatc tcgtgagcgt cgatctcttc aagaaccgta
     8401 ttcaccgctt cgatgttttc ttgtacacgc acatccgccg catcaatgac gtgcagcagc
     8461 aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg ccgccaccag atcgtgcggc
     8521 aggtggcgaa taaaccctac ggtatctgcc aacacggttt caccgacatc tgcgacgtca
     8581 atacgccgca acgtcgggtc gagggtggca aacaactggt ctgccgcgta gacccgcgct
     8641 tcggtgatgc gattgaaaag ggtagattta ccggcgttgg tatatcccac cagcgaaaca
     8701 gtaggaacgt cggctttgat acgcgattgc cgcccctgct cacgctgctt ttcaactctt
     8761 tccaggcgcg actgtatctg cacgatgcga ttacgcaaca aacgacggtc ggtttcgagc
     8821 tgggtttcac ccggaccacg caaacctatc ccgcctttct gtctttcaag gtgggtccag
     8881 ccacgcacca ggcgcgtagc cagatggcgc agctgcgcca gctcaacctg caacttaccc
     8941 tcatgggtac gcgcacgttg ggcgaaaata tctaaaataa ggccggtgcg gtcgataaca
     9001 cgacactcgc acaaacgctc caggttacgc tcttgcgccg ggctcagggc atggtcaaaa
     9061 agaacgaccg aagcacccgt cgctttgaca gcttccgcaa tttcaactgc tttaccttca
     9121 cctacaaaat actttgggtg cggcgcttta cggctaccgg taatcacctg caatgcttcg
     9181 acaccggcgg aagagaccag agattcaaac tcctggaggt cttccatatc tttgtcttgc
     9241 gtaaaataga tgtgtaccag taccgcctgc tcaccagcat cataacggtc aaacaagcgt
     9301 ataaccctct aaatagatca gcggggaacg caggatcgct ggctccccgt gtaaaaaaaa
     9361 cagcccgaaa ccttattcgg tttcttcgct gtcctgttgc gcggaagtat tctgcgcgct
     9421 gctaccatga tggtagttac tgctggtacc gccaccggcg ttgttactgt gatgagaaac
     9481 cgggcgagac gggacaacag tagaaatcgc gtgcttgtaa accatctggc tgaccgtgtt
     9541 tttcaacagg atcacgaact gatcaaaaga ctcgatttgc ccttgcagct taataccatt
     9601 caccaaataa atagaaactg gaacacgttc ccgacgcagt gcgttcagga acggatcttg
     9661 taaagattgc cccttagcca ttctctcttt tccttatatg cttatttgta ctttgaacct
     9721 ttcgattctg aaaaaattgc gcacgatacg tctcaattgt acacattcag cctgcgatag
     9781 caccaacaac ctgtaatact tcgtcacgcg cttgttctgg tttttcactg tcaagccagt
     9841 gaaccccttc ccaaccacgc agccaggtta tctgccgctt cgccaactgt ctcgtggcgc
     9901 aaacacctcg ataaaccatt tcatcgtatg agatttcgcc ttcaaggtaa gaccacatct
     9961 ggcgataacc cacgcaacga atggaaggca aatccgtatg caaatctcct cgggcaaaaa
    10021 gcgcccggac ttctgcttca aaacctgaag ccaacatctg atgaaaacgc tgctcaattc
    10081 gttgatggag cagttcacgg ctcgccgggg cgatggcgaa ctgatgcacc tgatacggta
    10141 gagcgtctcc tgacgtttgc gtcagttccg ttaaagtttt acccgaaatg aaaaaaactt
    10201 ccagtgcccg ggaaagcctt tgtggatcat ttggatgaat ccttgctgcc gcaaccggat
    10261 ctacctcctg aagttgacga tgcaatgact cccaaccttg ctctgccgcc tgttgctcaa
    10321 ttctggcccg cacttccggg tctgccgacg gtagcggcga caacccttcc agcaatgcct
    10381 tgaaatacaa catcgtaccg cccactaaca gtgggatccg ccccgccgcg gtgatatcgg
    10441 ccatttccgc cagcgcatcg cggcgaaaat cagcagccga gtaagcctgc gacggatcgc
    10501 gaatatccag caatcggtgc ggcgcggcga gtaactcttc agcgttcggc ttcgccgtcc
    10561 cgatatccat ccctttgtaa ataagggcag aatcaacgct tatcaactct actggtaaaa
    10621 ttttacgcag ctcaatggct aacgccgttt taccggaggc cgtcggcccc atcaaaaaaa
    10681 tcgccttagg caggctcgcc ttactgatat cactcatctt tcagggcttt tatcgccgga
    10741 tgtaaatcaa cagattgtaa cagaccaccc ggcggcgttt tcacaagttg cggacataac
    10801 cgttccacgt ccgccagcag ggttatggcc tgcgccattg accactgcgc atgttcgctc
    10861 atcagatttc gtgcaatcca ctgcgcaata ttgccaggtt cgaatacgga ctgcttcgcc
    10921 aggtagccta tcagttcagg aatcaagatt tgtaaatttt gttggcgtaa gggtaaaggc
    10981 acggccctga tggtcacatg ctgcgcatcg gactggaaat caatacccaa ttccgccagg
    11041 gcagactgcg ctttttctaa tgcagatttt tcttcgccag aaacttttag ccgcagcgga
    11101 atcagcaacg gctgggcgca aacgggcgct tcacccggcg tcaattgtgc ctgacgcagc
    11161 caacgttctg ccactggcaa ggctaaaagt gaaatgttgc cgtcgcgctc cagcaacgca
    11221 cagtcggaat ggacgatagt cagtacccga ccaaagcttt gactgttcgc cgcaagtgca
    11281 ggttcctgcg gttctggcgc ttttggtttt tgcatcggcg cgggcgtttg caaaagctgg
    11341 cgatacactt caccttgctg tttctggtaa cctggctgcg cattcggcca gggggcagcc
    11401 ggacgactgc ctgaggcggg cgcaggagtg taaagcggag ctactggctc acgaactgcc
    11461 ggttcagcaa aatgattgcg ccccgccgcc acgcggtttt ccgggatggc gcgcggtgca
    11521 ggttgtggtt catcgtccag cggtagcggc gtttccagtt gctgttgtag cacgctcagc
    11581 acgccctgat agataaagtc atgcaccaga cgcgactgat ggaaacgcac ttcgtgtttg
    11641 gcggggtgta cgttgacgtc cacctgatgc gggtcgatct ccagatacag cacaaacgcc
    11701 ggttgttgat cggcccccag tttgtcttcg caggcctggc ggatcgcatg attgatcagg
    11761 cgatcgcgca tcatgcgacc attcacgtag caatactgaa tttccgccag tgcgggcgtg
    11821 gtgtgattcg gatctgccac ccagccgcgc agcgtaagat cgccgtgttg ccattcaatc
    11881 gccagcgctt gttcgagaaa agcggtgccg caaatcgccc ctaaacggcg ctctttttgt
    11941 ccaccttccg gcacggcgcg gtactggcgg accattttcc cgttatgcga caggttgatc
    12001 gtgacgtcga aacgcgccag cgcaatgcgg cggatgatct catcaatgtg gttaaattcg
    12061 gttttctcgg tgcgcaggaa tttgcgccgc gccggggtgt tgtagaacag atccagcacc
    12121 tccagcgtag tccccacagg atgcgccgcc ggttttaccg tcacgtccat atcgcgccct
    12181 tcggcatagg cctgccaggc ttcctgctgt tctacggtgc gtgaagtgag cgtcaggcgg
    12241 gaaaccgaac tgatactcgc cagcgcctca ccgcgaaagc ccaggctgat aatggcttcg
    12301 agatcgtcca gagaggcgat tttactggtg gcatgacgag ccagcgccag cgccagctca
    12361 tcttttttga taccgcagcc gttatcacga atgcggataa gtttcgcccc gccacgttcg
    12421 atatcaatat caatgcgcgt cgcacctgca tcgaggctgt tttccaccag ttctttgact
    12481 accgacgcag gtcgctcgac cacctcacct gcggcaatct ggttcgccag ttgtggcggt
    12541 aagacctgta ttggcatcaa tcgtccttag tttggcagcg tacgatctgg cgtcgtcacc
    12601 gtactggcag tttgtgccgt tgcaccctgc ggcgcagatt gcatcggatg cgcaaggaaa
    12661 taattgcgca gacctttata aatggcttct gccagctgtt gttggtaatc gtcgctcgcc
    12721 agcaaacgtt cttcgctgtt gttgctgata aaaccggttt cgaccagtac tgatgggata
    12781 tccggcgaac gcagaacgcc aaggctggcg tgttctggtc gacgtttatg tatttcgcca
    12841 atgcgttgca actggctgat catactggtc gctacatcat accctacacg ctgggaatga
    12901 ccgaactgta aatccagcac cgcctggctt aaatagggat cagactgact gttcgccagc
    12961 acatcgcccg ctccgcccag cagctccgac tgtttctcgt gctgctccag ccagctggcc
    13021 atttcgctgt tggcgcgacg gttagagagc acccatacgg aagcgccagt cgcactgcgg
    13081 tttggtgcgg catcagcgtg aatcgacacg aggaaattgg cgttttgctt acgtgccaca
    13141 tcgctgcgcc ccatcaccga gataaagtaa tccccgtcac gggttaaaac gcctttaaac
    13201 attggatcgt cattgagaaa agtacgcaat ttacgtgcga tggcgatggt gacatttttc
    13261 tcccgcgtac caccggggcc gatagcgcca gggtcctgac cgccgtgtcc tgcatcaata
    13321 gcgatgataa ttttatcgcc agtgttagcc gtcgcgcgcg ctgccggacg cgttaccgta
    13381 ttactgctga taacacctgt agtgcggtta ctttccgttt taaacggatt gcgcgccggt
    13441 tcgctgacgc gcggtgcgac aaccgcaggc gtttcaacgc gtttagcaac cacaggcggc
    13501 gaaggaggcg gtggcggcac atcggcgtta atcgtaaaga cgacagtgta attgctgcca
    13561 ttctgccgct tcaccgcttc ggttttaccg ttttcggtta gatcgaccac cagccgtagc
    13621 gtttgtgcat ctttaggcgt tccggagcga atcgccttca ccagattatt gccgctaaac
    13681 aacaacggca gtccctgaat cacgcccgtt tgtttgatat cgagcgccac ggtgcgtttg
    13741 ctttgatggc taaacgcata atcaggatcg ccaataaaac tcaacgttat ccgcgcctgc
    13801 tgattgccgt tagaaacctg aatatcagag agcgtcgcgg cacccaccgg cgcgcacagc
    13861 agcagcaacg tcgctaccaa ccaatttctg atgcgataca tcatcccgcc acctttcaaa
    13921 ggttaaccgg ctaaacgcgc cagcaacaat tcacccgcag aggaaaccgc actcacgcgc
    13981 gcctcacggc cttgtgcctg ataatcaatg tgtatttcga catccgggtc aggaagaaca
    14041 cctgtacctt gttgtggcca ctccaccagg cagatggcat cgttggcaaa ataatcgcgg
    14101 atccccataa actccagctc ctcgggatcg gcaaggcggt acaaatcaaa gtgataaacc
    14161 attaaattgt cgagcgtata gggttcgacc agcgtataag tggggctttt gacattaccc
    14221 tgatggccca aagcctgcaa aaagccccgg ctaaaggtgg ttttacctgc gcctaaatcg
    14281 ccatacagat agattacggt tgcgccatcg caggctttcg ctacccgctc gcccaggtct
    14341 aatgttgcct gctcatcagg gagcggaatt actcgattca tcatggtttt tatcagtcac
    14401 ttccgggtta acaatacgct gtagcgtgga aaagagatcg gttgccagca tcccgcgcgt
    14461 tccaaaacgc gccgccagta cgtcagctgc cgcaccgtgc gcgacacagc ccgcacaggc
    14521 tgcatcatac ggcgacagtt tttgcccaag caatgcgcca ataataccag agagcacatc
    14581 gcccatgccg ccgctcgcca tgcctgcatt cccggcatca ataatgccta aagcgtcagg
    14641 atgggcggcg accacggttc cggcaccttt cagcaccgct acgccgccat aacgttgtac
    14701 cagacgtttg gcgcaatgta agcggtcact ttcaatttca gcgacggaac agcctaacaa
    14761 ccgtgcggcc tcgccaggat gtggcgtgat cacgcgattg tgacgcttat cgggattgat
    14821 tgccagcagg ttcaatgcat cggcatccca caacatcggt ttgcgaaaat tctcaacttt
    14881 ttgcagggct tttttccccc actcttgctg gcccagaccg ggaccaatca ccaccacatc
    14941 ggcccattcc aggctttcgg taagagagtc catagtcagt tcatgcacca tcaattctgg
    15001 tcgtgcagtc agcagcggcg caatgttctc actgcgggtc agtactcgga ctaaaccagc
    15061 accagcacgt agcgccgctt cccccgtcat acgaatagcc cccgccgtgc cgtgatcgcc
    15121 accgataatc accagccgcc cgtgatcgcc tttatgcgaa gtcgggcgac gcggtatcag
    15181 ccactgagaa agttgttctg ccgaaaaccg ctgaattttc gtctcctgac ctgccagcca
    15241 gctatccagc cccagtgagt caaaatgcag ttgtccggta acatcccgcg cttttccagt
    15301 gagcaagcct ggtttcagcg caataaaagt gatggtgtga tcggcgttga ttaccgcgcc
    15361 tggcgtagcg ccggtttcag ccagaaggcc ggaagggata tcaaccgcca caatcggcgc
    15421 agggtgagta ttagcgtggt cgattaactg gctaatggat tcgcggggcg cttgctgcaa
    15481 gccggtgccg agcagcgcat caacaatcag atctaccgat tcgggccaga caatattcga
    15541 agcatggatc tcgcctcccg cgtttaacca tgcttcgcgt gccagcgcgg cctcttccgg
    15601 caacggtttg tcactttcct gggccagcag cgtgacatca ataccgatcg ctttggccag
    15661 ccgcgcgacc acgtagccat cgccgccgtt attaccatga ccgcacagca ccagccagtg
    15721 gcgggcgtca ggataagccg aacgacacac ctggaatgcg gcttcgccag cgcgaagcat
    15781 cagctcatag agtgtgagcc ccagcgcatc tgccgcctcg cgttctccgc ggcggatatc
    15841 gtcggcgtac cagacggtgt gtggtatact tacggggttt ttcttcattg tatggtccgt
    15901 catgtcagag cccctcgatc tcaatcaatt agcgcaaaat attaaacagt gggggctgga
    15961 actgggcttt cagcaggtag gtattaccga taccgatctc agcgcgtccg agcccaaact
    16021 gcaagcatgg ctggacaaac aataccacgg cgaaatggac tggatggcgc gtcacggtat
    16081 gctgcgtgct cgtcctcacg aattattgcc cggtacgctg cgcgtgatca gcgttcggat
    16141 gaattacctt cctgctaacg ccgcatttgc cagcacgctg aaaaatccca aactcggcta
    16201 tgttagccgt tatgcgctgg gccgtgacta tcacaagctt ctgcgcaacc gactcaaaaa
    16261 gctgggcgag atgattcagc aacattgtgt ttcgctgaat tttagaccgt ttgtcgattc
    16321 tgcgcctatt ctcgagcgcc cgttagctga aaaagcaggt cttggctgga caggtaagca
    16381 ctcacttatc ctcaatcgcg aggccggttc gttcttcttt ttaggcgaat tgctggtcga
    16441 tattccgctg cccgtggatc aaccagtcga ggaaggatgc ggtaaatgcg tggcctgtat
    16501 gacgatttgc ccgaccggtg ccatcgtcga gccatatacc gtcgatgctc gccgctgtat
    16561 ctcttatctc accatcgaac tggaaggggc gatcccggaa gagttgcgac cgttaatggg
    16621 aaaccgtatt tacggttgcg atgactgcca gcttatctgc ccgtggaatc gctattcgca
    16681 actcactaca gaagacgatt tcagcccgcg taagccgcta cacgcaccgg aactcattga
    16741 gttattcgcc tggagtgaag agaagttttt aaaagtcacg gaaggttcgg cgattcgccg
    16801 tataggtcac ctgcgttggc tgcgtaatat cgccgtagca ttaggcaatg ccccctggga
    16861 tgaaacgatt ttgacagcgc tggaaagtcg taaaggtgag cacccacttc ttgatgagca
    16921 catagcgtgg gcgattgcgc agcaaatcga gaggcgaaat gcgtgcgtgg tcgaagtgca
    16981 attacccaaa aaacagcgtc tggttcgggt gattgaaaaa gggttaccgc gtgacgcctg
    17041 attcatccac agcttgtgaa taaaaataaa aacgtattgc aattcaagac gaataaaatc
    17101 gtcaagtgat cgactcgaca aaacgaaatc ttaattttta acttatctat caataggtta
    17161 aaaaaatact attcaccgtg cgaagtttct ttgaaattaa gattacggcc tgaggctgtg
    17221 gataactctg tttacaagag tttgtcagag acaacaaaaa ccagcaataa cgcatcgcgt
    17281 cgctgtggat attttattga gagaaga
//
LOCUS       DAZKGF010000103        17303 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZKGF010000103 DAZKGF010000000
VERSION     DAZKGF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.103"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(54..455)
                     /gene="crl"
                     /locus_tag="ACHOQV_003222"
     CDS             complement(54..455)
                     /gene="crl"
                     /locus_tag="ACHOQV_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP9631552.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(513..1757)
                     /gene="frsA"
                     /locus_tag="ACHOQV_003223"
     CDS             complement(513..1757)
                     /gene="frsA"
                     /locus_tag="ACHOQV_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP9631553.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRYWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(1849..2307)
                     /gene="gpt"
                     /locus_tag="ACHOQV_003224"
     CDS             complement(1849..2307)
                     /gene="gpt"
                     /locus_tag="ACHOQV_003224"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9631554.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            2568..4025
                     /gene="pepD"
                     /locus_tag="ACHOQV_003225"
     CDS             2568..4025
                     /gene="pepD"
                     /locus_tag="ACHOQV_003225"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP9631555.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(4082..4434)
                     /gene="prfH"
                     /locus_tag="ACHOQV_003226"
                     /pseudo
     CDS             complement(4082..4434)
                     /gene="prfH"
                     /locus_tag="ACHOQV_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(4430..4636)
                     /locus_tag="ACHOQV_003227"
                     /pseudo
     CDS             complement(4430..4636)
                     /locus_tag="ACHOQV_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(4954..5406)
                     /gene="yafP"
                     /locus_tag="ACHOQV_003228"
     CDS             complement(4954..5406)
                     /gene="yafP"
                     /locus_tag="ACHOQV_003228"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9631556.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFSMRYKPQH"
     gene            complement(5403..6458)
                     /gene="dinB"
                     /locus_tag="ACHOQV_003229"
     CDS             complement(5403..6458)
                     /gene="dinB"
                     /locus_tag="ACHOQV_003229"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP9631557.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(6529..7314)
                     /gene="lafU"
                     /locus_tag="ACHOQV_003230"
     CDS             complement(6529..7314)
                     /gene="lafU"
                     /locus_tag="ACHOQV_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFP9631558.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            7286..8998
                     /locus_tag="ACHOQV_003231"
                     /pseudo
     CDS             7286..8998
                     /locus_tag="ACHOQV_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(9216..9713)
                     /gene="rayT"
                     /locus_tag="ACHOQV_003232"
     CDS             complement(9216..9713)
                     /gene="rayT"
                     /locus_tag="ACHOQV_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFP9631559.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKQ
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLQNMWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVAKGLYPIDWAGDVTDFSAG
                     ERIIL"
     gene            complement(9890..10639)
                     /gene="yafL"
                     /locus_tag="ACHOQV_003233"
     CDS             complement(9890..10639)
                     /gene="yafL"
                     /locus_tag="ACHOQV_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9631560.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            10951..11691
                     /gene="dpaA"
                     /locus_tag="ACHOQV_003234"
     CDS             10951..11691
                     /gene="dpaA"
                     /locus_tag="ACHOQV_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFP9631561.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(11662..12429)
                     /gene="yafJ"
                     /locus_tag="ACHOQV_003235"
     CDS             complement(11662..12429)
                     /gene="yafJ"
                     /locus_tag="ACHOQV_003235"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFP9631562.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(12545..13123)
                     /gene="lpcA"
                     /locus_tag="ACHOQV_003236"
     CDS             complement(12545..13123)
                     /gene="lpcA"
                     /locus_tag="ACHOQV_003236"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFP9631563.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13363..15807
                     /gene="fadE"
                     /locus_tag="ACHOQV_003237"
     CDS             13363..15807
                     /gene="fadE"
                     /locus_tag="ACHOQV_003237"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFP9631564.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(15850..16296)
                     /gene="ivy"
                     /locus_tag="ACHOQV_003238"
     CDS             complement(15850..16296)
                     /gene="ivy"
                     /locus_tag="ACHOQV_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFP9631565.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            16477..17247
                     /gene="yafV"
                     /locus_tag="ACHOQV_003239"
     CDS             16477..17247
                     /gene="yafV"
                     /locus_tag="ACHOQV_003239"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFP9631566.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWRDADEFRLW"
BASE COUNT         4127 a         4499 c         4521 g         4156 t
ORIGIN      
        1 acgtatcagt tttaatgaat attgccggat gtgatgcatc cggcacattt cattcacgcc
       61 gttaacttca ccggttcatc acgaaaatca tctgccggtt ccagcttcag attcagcgtc
      121 gccagcagct cacgcagctt ctcgtgaaat tcacgcaggg tgtgctccag tcgttcaacc
      181 acttcggcat cttttatctg aacactcgtc cagtcgcctg ctttatcgaa cagaccaaac
      241 tggtaactgt aggtaaaacg ggattcctgc gcttccagtt ccatccacca gccccagaac
      301 tcacgcactt ccggtgccgg tttcacgttg acgcatacag ccagacaatc gaaaaagaat
      361 cgattatctt cgcacttacc ttcacgaata tacgggccta gtgcggtaaa ttttttgatc
      421 aatctgctct tcgggtgtcc actcggtaac gtcattgcaa tctcctgttg tgatgcaact
      481 gttttaccaa attggtaaaa tttagcaaat ttttaacaca aacgtttttc gatccagtct
      541 gtgatttcct gaaggccttt gtcaaaattc cgatacaccg ggttaaatgg gatctctaat
      601 aatttaccgt cagcagatga cgacgtgatt aagcgtgagt cctcttccgg gctgaacgga
      661 tcgttcttcc agtagcctga taacattggc gttgggcagc gacgtcccag caatccttgc
      721 acttttaatg aatagcgatt caactccact cgcaacgctt catcggaagc atcatgcatc
      781 cctaaacgac tggccagaac gtcaagatac atttccggca cctgttgctg gcacttaaaa
      841 tcactcaaca gggtatgaac taccggacca agacaggcaa ccactttcag acgcggcgat
      901 tcaaggtatg ccagacgcac ggcaacgtta gcgccgaaac ggaaaccaaa ggccgcgacg
      961 cgagtgtgat ccacccacgg tacgttaggc agcgccttta aaacgtgctg atgcaacagg
     1021 ctggagtcct gggtgagctt ccattttgaa gaaaagccca ccgacggcat atcaatagtc
     1081 agcatagcaa tgccgcgcgg cgcaaaataa cgttcataca ggctgtaata gtccgtctgc
     1141 atcgcatcca gaccaccaca cattaatact gtcgggaacg ggccatcgcc tttcggcata
     1201 tgcaaaaagc cggtgatggg cgcaccgccg ggtacggtaa actccatctg ccgcatcgtg
     1261 cccggcagac gctgagcggc ctcttcatag gcgcggtttg acaaagcctg cgcttgctcg
     1321 gccaggtcat ctcctttcag atgaggatag gcagcaatgt tgtacaacgt agccgcatgc
     1381 agccagtatc gaccgctgag ttgcggatct tcttccgcac aggctttttg ttgccacacc
     1441 atcgcctggg tagcccactc ataaatccag ttgccgccgc ggtagccaat caccgtatcg
     1501 tataaatcat cgtcggtacg ttcggcatcg ctcatcacaa tacgcgcctg gacgtcgagg
     1561 atttcacggg ggtcaatgcc gcgccagatc cacatcagac ggttaatcat gcgataccag
     1621 tgagggatgg ttttaccatc aagggccgac tgcacaggca gttgtgcgcc gtgactaaag
     1681 cggcggacta gcgtcgaggt ttcaggatgt ttaaagcggg gtttgaacag ggtttcgctc
     1741 aggtttgcct gtgtcatgga tgcagcctcc agaatactta ctggaaacta ttgtaacccg
     1801 cctgaagtta aaaagaacaa cgcccggcag tgccaggcgt tgaaaagatt agcgaccgga
     1861 gattggcggc acgaatacga cgcccatatc ccacggctgt tcgatccagg tattttgcgg
     1921 gatatcaaca acataatcat caaccagcgg acgaccagcc ggttttgcga agatggtgac
     1981 aaagtgcgct tttggataca tttcacgaat cgcaaccgca gtaccaccgg tatctaccag
     2041 gtcatcaata acgatgaagc cttcgccatc gccttctgcg cgtttcagca ctttaagctc
     2101 gcgctggttg tcgtgatcgt agctggaaat acaaacggta tcgacatgac gaatacccag
     2161 ttcacgcgcc agtaacgcac ccggtaccag accgccacgg cttacggcaa taatgccttt
     2221 ccattgttca gaaggcatca gtcggcttgc gagtttacgt gcatggatct gcaacatgtc
     2281 ccaggtgacg atgtattttt cgctcatgtg aagtgtccca gcctgtttat ctacggctta
     2341 aaaagtgttc gaggggaaaa taggttgcgc gagattatag agatctggcg cactaaaaac
     2401 cagtatttca catgagtccg cgtcttttta cgcactgcct ctccctgacg cgggataaag
     2461 tggtattctc aaacatatct cgcaagcctg tcttgtgttg acaacatttt ctgctaaccc
     2521 tgtgacctgc aatactgttt tgcgggtgat cgacaaggag acttaacgtg tctgaactgt
     2581 ctcaattatc tccacagccg ctgtgggata tttttgccaa aatctgttct attcctcacc
     2641 cgtcctatca tgaagagcaa ctcgctgaat acattgttgg ttgggcaaaa gagaaaggtt
     2701 tccatgtcga acgcgatcag gtaggtaata tcctgattcg taaacctgct accgcaggta
     2761 tggaaaatcg taaaccggtc gtcttgcagg cccacctcga tatggtgccg caaaaaaata
     2821 acgacaccgt gcatgacttc accaaagatc ctatccagcc ttatattgat ggcgaatggg
     2881 ttaaagcacg cggcaccacg ctgggtgcgg ataacggcat tggtatggcc tctgcactgg
     2941 cggttctggc tgacgaaaac gtggttcacg gcccgctgga agtgctgctg accatgaccg
     3001 aagaagccgg tatggacggt gcttttggct tacagagcaa ctggttgcag gctgatattc
     3061 tgattaacac cgactccgaa gaagaaggtg aaatctacat gggttgtgcg ggaggtatcg
     3121 acttcacctc caacctgcat ttagatcgtg aagcggttcc agctggcttt gaaaccttca
     3181 agttaacctt aaaaggcctg aaaggcggtc actccggcgg tgaaatccac gttggcctgg
     3241 gtaatgccaa caaactgctg gtgcgcttcc tggcgggtca tgcagaagaa ctggacctgc
     3301 gccttatcga tttcaacggc ggcacactgc gtaacgccat cccgcgtgaa gcctttgcga
     3361 ccattgctgt cgcagctgat aaagtcgacg ctttgaaatc tctggtgaat acctatcagg
     3421 agatcctgaa aaacgagctg gcagagaaag agaaaaatct ggccttgttg ctggactctg
     3481 ttgcgaacga taaagctgcc ctgattgcga aatctcgcga tacctttatt cgtctgctga
     3541 acgccacccc gaacggtgtg atccgcaact ccgacgtggc aaaaggtgtg gtcgaaacct
     3601 ccctgaacgt cggtgtggtg accatgactg acaataacgt agaaattcac tgcctgatcc
     3661 gttctctgat cgacagcggt aaagactacg tggtgagcat gctggattcg ctgggtaaac
     3721 tggctggcgc gaaaaccgaa gcgaaaggcg catatcctgg ctggcagccg gacgctaatt
     3781 ctccggtgat gcatctggta cgtgaaacct atcagcgcct gttcaacaag acgccgaaca
     3841 tccagattat ccacgcgggc ctggaatgtg gtctgttcaa aaaaccgtat ccggaaatgg
     3901 acatggtttc tatcgggcca accatcaccg gtccacactc tccggatgag caagttcaca
     3961 tcgaaagcgt aggtcattac tggacactac tgactgaact gctgaaagaa attccggcga
     4021 agtaattatt tgatttgctg ccggatggcg tttaatcgcc ttccggcagt ttcatccttc
     4081 attatccttc gataaaagcc atccctgtga acgttcgtcg cgggttgcca cgttcaatct
     4141 ggtgatggaa cattcgccgc tgcgatttca gcgccgcgct attttcctgt tgctgttgtt
     4201 ccagcttcca ggcaatcagc aatcgcgcca gccgcttgtt agcatgccca gaaaccagtt
     4261 tttgcgccca tgatgaggcc gatacggact cggacaaata cactgaatag tgccgcacca
     4321 actttcgctt aatgcccatg cattatcgcc atccagagaa atcagcgccg aacgcagcgt
     4381 gtcagagtag cggcccgttt ctgtttccag caccgttacc gcgacgtctt gccgggtaaa
     4441 aacgaccaac agagaagaac gctgcgccaa tcgggtagcc gcgtccggga tgtaaatctg
     4501 gcatccccac aacgcgctgc atgttgggta aatttgccgt ggtgtgtaat tgttggatcg
     4561 ctaaactctc gatccacagg tcatcagatg cgatggtaaa taccgcatcg gataagggac
     4621 gaatatattt gcccatgtat cattccattt atactcgtca tacttcaagt tgcatgtact
     4681 gcgaccgcgt tcgttcaccc cagtcactta ctttagtaag ctcctgggga ttcactcgct
     4741 tgtcgccttc ctgcaactcg aattatttag agtatataaa tataaaaatc aggaaatggc
     4801 agacgaatcc agggcaagaa aaaaagaatt aatcctggaa aggtctgcaa agagtcattg
     4861 ataaaggatt tgtcatgatt tacctccttg cagtttgtgg aataagaaaa cgcgcacata
     4921 ccaggcgggt gttattttca ttgcaagctg gatttaatgt tgcggtttat atcgcataga
     4981 aaaattagta aaccacgctc ccctgcattc aacgtgctgc tgcttaactg tctgaaaacc
     5041 ataacgttca aaaaagggtt ttgcggttat gctggcgtcc accgtaagtt cggattcaga
     5101 cttaatcaaa ggttttaaca aggcgctggc aaccccgcgg cgggtgtatt cagggtcaac
     5161 aaataacata tcgatatagt gttcaacgca ggtaataaag ccgaccagtt ttgcattaat
     5221 gactgcaacc cgcacttgtg atttcgcgag tttctccttc cagcgagatt cgtcaatctg
     5281 cgcccaggcg gcaatttgtt gtggtgaata atgctgactg gcggtcatcg taaccgctct
     5341 aaggaaaata gcgcatagtt gctgaaaatc gccaggctga tagtctctta tttgtatgtt
     5401 attcataatc ccaacaccag ttgtctttcc atttgcgggt caagtaacgt cacatgcagc
     5461 cccaccagtc gcacaccgcg cccgccgcgg cgttcatccc aggttttacg cgcagtggcg
     5521 attagatcag ctttattcag ccgtggccag acgtgctcct gagtagtttg ctgaaaatcg
     5581 tcgaacttta atttcactcc ctgacgggcg atcagtaaat caggtttcac ctttgccaga
     5641 cggcgttcaa gttcaggata cagccgctcg ataatcgctt cacattcaga ccagtgatga
     5701 atatcttccg ccatcgtgcg ttccacgccg acggattttc gcaaccgttc actgttaacg
     5761 tcgcgctcgt caatcccctg actacgctcc cacaaaatgc ggccaaattt gccaaagcgt
     5821 ttaagcagaa tcaccagatc acacttttgc acatcaccgc aggtccgcag ccccatcgct
     5881 tccagttttg ccgccgagac tttgccgacg ccgggaattt ttgccagcgg taaggtttgc
     5941 agaaatgccg gaacttctgc cggcgtaatc acaaactgac cgttgggttt attcatgtcg
     6001 gaggcgattt tggcgagaaa ctttactggt gccacgcccg cagatgccgt cagttgcagc
     6061 tcgttgaaga tcgtctggcg gatttcctgg gcgatgaggg tcgcagaacc gtggcaatgg
     6121 acgctatcag tgacatcgag ataggcttca tccagtgaca acggttcaat acgcgaggtg
     6181 tagcgcgaga agatttcacg gatatgattt gaggcttctt tgtaggcgtc aaagcgcccc
     6241 ggaagcaagg tgagatgcgg gcataatttg agcgccatcc ctgtcggcat agcgctacgt
     6301 acgccaaatt tacgcgcggg ataattggcg gtgctgatta ccccccgtcg ttcgcggctg
     6361 ccgccaatag caatagggat atcgcgcagg gcgggattgt cgcgcatctc caccgctgcg
     6421 aaaaagcagt ccatatccac atgaatgatt ttacgcattg ctcacctctc aacactggta
     6481 aagtatacag tgatttcagg gtttgagaaa tgcgtaaaga ttcagcattt aacgcgctcg
     6541 ctgcgaaagc acctgctgct tatccagctt ttgcaccagc ggctgcacca ctttatcccc
     6601 atgctgacca aagtattgat acagcgtatc tgacgcactt ttggtcagca ccataatctc
     6661 aatgcgccgg ttgcccgcgc tttgcggatt tttggcatcc agcagcatct ggtccgccat
     6721 tgcgcttacc tgcatcactt tattttccgg cattccggcc tcttccagca cccgacgagc
     6781 cgaaagcgcg cgatcgcccg aaaggttcca gttgttgtag atattgtttt tgtaggccat
     6841 cgcatcggta tgcccggtaa taataatttt gttatcgagc gagtcgaaca ctggcgcgag
     6901 ctccaccagc agcgttttaa agaacggcat aatcttcgcg ctgccgcgtt caaacatatt
     6961 gcggttctgg tcatctttaa tcagcacgcg cagtccctgg ggaacaatct ccatctccag
     7021 attcgcttcc atatgtgcat cacgggcaat ggtgttgata ctggtcgcca gctcgccaag
     7081 ctcggtggcg gatttcttct tcagcagcgc cgttttctcg ttgacgtcgc gcgctttttt
     7141 ctccgtctct tccggcaccg ccacggtggc aggttttggc ggatgagacg ggctgatttt
     7201 atttaacggc gacaacccac caccgttaaa aatcgactgc ccgtgcagcg cggcgataat
     7261 gctttcacgc tcggatttac tgacgctatt gacaatcaac tttatcgtcg tcaccaaagg
     7321 ggccgagcgt atttccgagg tttccgcccg ctttacctta gacgcgatgc ccggtaagca
     7381 gatggcgatt gacgccgatc ttaacgccgg gttgatcaac caggcgcagg cgcaaacccg
     7441 gcgtaaagat gttgccagcg aggccgattt ctacggcgca atggacgggg catcgaagtt
     7501 tgtgcgcggg gacgccatcg ccgggatgat gattctggcg atcaacctga tcggcggcgt
     7561 ctgtatcggg atcttcaaat acaacctgag cgccgacgcc gccttccagc aatatgtgct
     7621 gatgaccatc ggcgacgggc tggtggcgca gatcccttcc ctgctgctct ccaccgcggc
     7681 ggcgattatc gtcacccgcg tcagcgacaa cggcgatatc gcccatgacg tgcgccacca
     7741 gctgctggca agcccgtcgg tgctctacac cgccaccgga attatgtttg tactggcggt
     7801 ggtgccggga atgccgcacc tgccgttttt gctgttcagc gccctgcttg gctttaccgg
     7861 ctggcggatg agcaaacgcc cgcaggcggc ggaggcggag gagaaaagtc ttgaaacgct
     7921 aacccgcacc atcactgaaa ccagcgagca acaggtcagt tgggaaacca ttccgctgat
     7981 cgagcctatc agcttaagcc tcggctacaa gctggtggcg ctggtcgata aagcgcaggg
     8041 gaacccactc acccagcgga ttcgcggcgt gcggcaggtg atatccgacg gcaacggcgt
     8101 gctgctgccg gagatccgta ttcgggaaaa cttccgcctc aagcccagcc agtacgccat
     8161 attcatcaac ggcattaagg ccgatgaagc ggatattccg gcagataaac tgatggccct
     8221 gccctccagc gaaacctacg gcgagattga cggcgtactg gggaatgacc cggcatatgg
     8281 gatgccggtc acctggattc agcctgcgca gaaagcgaag gcgctgaata tggggtatca
     8341 ggtgatcgac agcgccagcg tgattgccac ccatgtgaat aagattgtgc gcagctatat
     8401 tcctgattta tttaactatg acgatattac gcagttgcat aaccgcctgt cgtcgatggc
     8461 accgcgactg gcggaagact taagcgcagc gctcaattac agccagttgc tgaaagtgta
     8521 tcgcgcgctg ctgaccgaag gcgtttccct gcgcgatatt gtcaccatcg ccaccgtgct
     8581 ggtcgccagt agtgcggtga ctaaagatca tatcctgctg gcggcagatg tgcgcctggc
     8641 gctgcggcgc agtatcaccc atccgttcgt tcgcaagcag gagctgacgg tgtatacgct
     8701 gaataatgag ctggaaaatc tgctgaccaa cgtggtgaat caggcgcaac aggccggcaa
     8761 agtgatgctc gacagcgtgc cggtggaccc gaatatgctt aaccagttcc agagtacgat
     8821 gccacaggtg aaggagcaga tgaaagcggc ggggaaagac ccggtgctgc tggtgccgcc
     8881 gcaactgcgt ccattgctgg cgcgctatgc aaggttgttt gcgccggggc tgcatgtgct
     8941 gtcgtataac gaagtgccgg atgagctgga gttgaagatt atgggggcgt tgatgtaaga
     9001 atccggcatc ggtgcacaat tgccggatgc ggcgtgaacg ccttatccgg cctaccgtta
     9061 aggcatagat ttgtaggcat gataagacgc gctagcgtcg catcaggcat ctgcgcacaa
     9121 ccgccggatg cggcgtgaac gccttatccg gcctaccgtt aaggcacaga tttgtaggcc
     9181 ggataagcgt aagcgcatcc ggcaccggcg cacaattata aaataatacg ctccccggca
     9241 ctaaaatccg ttacgtcccc cgcccaatcg atgggatata aacctttcgc gacatcgcga
     9301 tggaacgttg agaatggcca atcactcacc tgctttaccc agccatgttt cactggattt
     9361 atgtaaatat aatcaacatg atgacgataa tctttggtat tgcgaatggc atgctcccaa
     9421 aaacgcggtt gccacatatt ttgcaatcca caagcatggg tgaactgttt tttaatctcc
     9481 cgccagcgag aggaaaaatc atcatcgctt tcgggtaatg tccagataca gtgcatatgt
     9541 tccggcaaaa cgacccaggc gttaatttca aaaggtctgg cctgcttaac tttaataatg
     9601 gcgttacgga gtgtctggta ctgggtggtc aaaagttggc ttcgacgatt ccgtaaattt
     9661 accgtgaaaa accatgttcc ccccttgatg taataacgac gatattcaga catggcgact
     9721 cctttttctc aacgataaga agaaaaggaa aagtcgacaa tgaagttgtg ttatatcggg
     9781 aaagcagctc gcaaaaaatt cgcagactcg taggcatgat aagacgcggc agcgtcgcat
     9841 caggcatcgg tgcactattg ccggatgcgg cgtgaacgcc ttatccgtcc tacaaaatcg
     9901 tttcctccgt caaaatcctg cgcgcgccta aaaaatggtc ctgccagaaa ggttcggcta
     9961 accgacttac ccgaatggtt tccccggtgc gcggtgactc gataaactga ccgtctccaa
    10021 gatatacgcc catatggtcg gctatctcac gactgtggat atggaaaaac agcaaatctc
    10081 cccggcgcag gtcgttattc gccacaatcg ttgcccgatg gtagtgatac atctcattgg
    10141 ccgtacgcgg gagcttcgcc tcgaggatct tgttgtaggc ataaaaaacc aacccgctac
    10201 agtcgaagcc ttgatcgggc cgcgtgccgc cccagacgta aggcttacca agctgctgct
    10261 ccaggcggtg aatcgccact tcggtgatgt tgtgtagatg gtcgctgctt aagaaatggt
    10321 tgttctccgc cagcgcctgc cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt
    10381 ctggatgctg ttttatctgc tcccgattat gctggcgcag cgcccttctg cttttggcat
    10441 tgccttccac aatatagctg gcctgctttt tcagatgggt ttggtactgt tttaataaac
    10501 gcgcacggtt ttgcatccgc tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg
    10561 cgctggcgag taaagggagc aaaacgcata acgaaaacac gcctggatgc agaaatctgc
    10621 cgtgcaagaa cgaagacatg aaggacataa accagctgaa tgaaggtgag aattttaaaa
    10681 gaccggagga gtaaacggga ttattaattg cttatttgca tattactatt gcggtttcat
    10741 aatcttagga aataagaaat aaatctacaa tccatgcgct tttcggactc ttgaaaactg
    10801 cccctgtgct aaatttgtaa accctgcgcc gacaggcttg cgtaaaactc ttttggtgat
    10861 aatgtgagcc ttccaaatcc agactattcc gatttgggat tcattttgcc gtcctggcgt
    10921 gtgtaaccgt tttatcaagg aataagcagt atgcgtaaaa tcgcattaat tcttgcgatg
    10981 ttgttgatcc cgtgcgtttc gtttgccggt ttgctgggca gcagtagctc gaccacgcca
    11041 gtgagcaaag agtataagca gcagttgatg ggatcccctg tctacatcca gatcttcaag
    11101 gaagaacgta cgctcgatct ctacgtcaaa atgggcgagc aatatcaact gctcgacagc
    11161 tataaaatct gtaaatattc cggcggctta gggccaaaac agcgtcaggg cgatttcaaa
    11221 agcccggaag ggttttatag cgtccagcgt aatcagttaa aaccagacag ccgttactac
    11281 aaagcgatta atattggatt cccaaacgcc tatgaccgtg cgcatggtta tgaagggaaa
    11341 tacctgatga ttcacggcga ttgtgtttcc atcggctgct acgcgatgac caatcagggt
    11401 attgatgaga tcttccagtt cgttactggt gcgctggtgt ttggtcagcc gagcgtgcaa
    11461 gtgagtattt acccgttccg tatgaccgac gccaatatga agcgccataa atattccaac
    11521 tttaaggact tctgggagca actgaagcca ggctacgact actttgagca aacccgtaag
    11581 ccaccgaccg tttctgtggt caatggccgt tacgtggtca gcaagccgtt aagccacgaa
    11641 gttgtgcagc cacaactggc atcaaactac acgctccccg aggcaaaata agcgccattc
    11701 acctggcata atcttttgcc aggtttcatt gcccgtcagc ggctgtgtcg caatcaccgt
    11761 gaccacatca ttcggtgtgg tctgcgagct gaagtcgatt tccacatcct gatccagcaa
    11821 cgttgccacg ccaaacggcg cgcggcgggt gatccagtgt aaattagtcg agcaatacgc
    11881 cattacatag cgcccgtcag aaagcagcat gttgaaaacg cccttctgcc gcagttcatc
    11941 cgccagtgag gcgatatatt taaacactgc cgccatgttg cccggtgtgc gcgggtaacg
    12001 ctgcgttaat ttatgcagga gccagcaaaa ggctttttcg ctatcggttt caccgaccgg
    12061 gcggaagttg ccggtttcca gtgatttgta gcccgtcagt tgtccattat gggcataagt
    12121 ccagttacgc ccccataact cgcgggtaaa cgggtgagta ttttccagcg ccacctcgcc
    12181 ccgattagcc tggcgaatat gagccaccac cgaacaggat tttatcgggt agtcctggac
    12241 aagtttggcg atgggggaat taaagctggg ttgcggatct ttaaatgtgc gacagccttt
    12301 accttcgtaa aaggtaatgc cccagccatc tttatgtggc ccggttccac caccacgctg
    12361 tacaagtccg gtgaaactaa agcagatatc ggtagggacg ttggcgctca tcccgagcaa
    12421 ttcgcacatg ggtaacctcc acaacagcgg gagcgcatgg cccccggcga agattgatta
    12481 aaaagtacaa aggttcaggc tgttaccctc accccggccc tctccctgaa agggagaaaa
    12541 gaccttactt aaccatctct ttttcaatta actggatcag gatatggatc actttaatgt
    12601 ggatctcctg aatgcggtcg gcataaccaa agtgcggtac gcgaatttcg atatccgctg
    12661 tgccagccat tttgccgccg tctttaccgg tcagggtgat cactttcatc cccttctcac
    12721 gcgccgccgc gatcgctttg atcacgtttg cagagttacc ggaggtggag atccccagca
    12781 gtacatcgcc ttcgcgacct accgcttcaa cgtaacggga gaaaatatca ttgaaaccaa
    12841 aatcgttacc gacgcaggaa atatggctga cgtcagaaat ggcaatcgcc gggtagcccg
    12901 gacggttttc acggtagcga ccggtcaact cttcggcaaa gtgcatagcg tcgcaatggg
    12961 agccgccgtt gccgcaggaa agcactttgc caccggcttt aaagctgtct gctaacagga
    13021 ccgccgcgcg ctgaatggcg tgaatattgg cgtcatcttt taaaaaatta gccagcgttt
    13081 ccgccgcttc gttcagttcg ttacgaataa gatcctggta catgaggata tccttcagca
    13141 taaatgtaat agacaaaatg cagtgtaccg gataccgcca aaagcgagaa gtacgggcag
    13201 gtgctatgac caggactttt tgacctgaag tgcggataaa aacagcaaca atgtgagctt
    13261 tgttgtaatt atattgtaaa cgtattgcta aatgttttta catccactac aaccatatca
    13321 tcacaagtgg tcagacctcc tacaagtaag gggcttttcg ttatgatgat tttgagtatt
    13381 ctcgctacgg ttgtcctgct cggcgcgttg ttctatcacc gcgtgagctt atttatcagc
    13441 agtctgattt tgctcgcctg gacagccgca ctcggcgttg ctggtctgtg gtcggcgtgg
    13501 gtactggtgc ctctggccat tatcctcgtg ccatttaact ttgcgcctat gcgtaagtcg
    13561 atgatttccg cgccggtatt tcgcggtttc cgtaaggtga tgccgccgat gtcgcgcact
    13621 gagaaagaag cgattgatgc gggcaccacc tggtgggaag gcgatttgtt ccagggcaag
    13681 ccggactgga aaaagctgca taactatccg cagccgcgcc tgaccgcaga agagcaagcg
    13741 tttctcgacg gcccggtaga agaagcctgc aggatggcga atgatttcca gattacccat
    13801 gagctggcgg atttgccgcc ggagctgtgg gcgtatctta aagagcatcg cttcttcgcg
    13861 atgatcatca aaaaagagta cggcgggctg gagttctcgg cttatgccca gtctcgcgtg
    13921 ctgcaaaaac tctccggagt gagcgggatc ctggcgatta ccgtcggcgt gccaaactca
    13981 ttaggcccgg gtgagctact gcaacattac ggcactgacg agcagaaaga tcactatctg
    14041 ccgcgtctgg cgcgtggtca ggagatccct tgctttgcac tgaccagccc cgaagcgggt
    14101 tccgatgcgg gcgcgattcc ggataccggg attgtttgca tgggcgagtg gcagggccag
    14161 caggtgctgg ggatgcgact gacctggaac aaacgctaca ttacgctcgc gccgattgcg
    14221 accgtgcttg gtctggcgtt taaactctcc gacccggaaa aactgctggg tggcgcagaa
    14281 gatttaggta ttacctgtgc gctgatcccg accaccacgc cgggcgtgga aattggtcgt
    14341 cgccacttcc cgctgaacgt gccgttccag aacggaccga cgcgcggtaa agatgtcttc
    14401 gtaccgatcg attacattat cggcggaccc aaaatggccg ggcaaggctg gcggatgctg
    14461 gtggagtgcc tctcggtagg ccgcggtatc accctgcctt ccaactcaac cggcggcgtg
    14521 aaatcggtag cgctggcaac gggtgcgtat gcgcacatcc gccgtcagtt caaaatctct
    14581 attggtaaga tggaagggat tgaagagccg ctggcgcgta ttgccggaaa cgcttatgta
    14641 atggatgctg cggcatcgct gattacctac ggcattatgc tcggtgaaaa acctgccgtg
    14701 ctgtcggcta tcgttaagta tcactgtacc caccgtgggc agcagtcgat tattgatgcg
    14761 atggatatca ctggtggcaa aggcattatg ctcgggcaaa gtaacttcct ggcccgtgct
    14821 taccagggcg caccgattgc catcaccgtt gaaggggcta acattctgac ccgcagcatg
    14881 atgatcttcg gacaaggagc gattcgttgc catccgtacg tgctggaaga gatggaagcg
    14941 gcgaagaaca atgacgtaaa tgcgttcgat aaactgctgt tcaaacatat cggtcacgtc
    15001 ggtagcaaca aagttcgcag cttctggctg ggcctgacgc gcggtttaac cagcagcacg
    15061 ccaaccggcg atgccactaa acgctactat cagcacctga accgcctgag cgccaacctc
    15121 gccctgcttt ctgatgtctc gatggcggtg ctgggcggca gcctgaaacg tcgtgagcgt
    15181 atctcggcac gtctggggga tattttaagt cagctttacc tcgcctctgc cgttttgaag
    15241 cgttatgacg acgaaggccg caacgaagcc gacctgccgc tggtgcactg gggcgtacag
    15301 gatgcgctgt atcaggcaga acaggcgatg gacgatttac tgcaaaactt cccgaaccgc
    15361 gtggttgccg ggctgctgaa tgtggtgatc ttcccgaccg gacgtcatta tctggcacct
    15421 tctgacaagc tggatcataa ggtggcgaag attttacaag tgccgaacgc cacccgttcc
    15481 cgcattggtc gcggtcagta cctgacgccg agcgagcata acccggttgg tttactggaa
    15541 gaggcgctgg tggatgtgat tgccgccgac ccgattcatc agcggatctg taaagagctg
    15601 ggtaaaaacc tgccgtttac ccgtctggat gaactggcgc acaacgcgct ggcgaagggg
    15661 ctgattgata aagatgaagc cgctattctg gtgaaagctg aagaaagccg tctgcgcagt
    15721 attaacgttg atgactttga tccggaagag ctggcgacga agccggtaaa gttgccggag
    15781 aaagtgcgga aagttgaagc cgcgtaattg aataacggag ccgaaaggct ccgtttcttt
    15841 atccgctaat tatttaaaat taaagccatc cggatggttt tccaggctgc cggtcaacgc
    15901 cgcgaacagc accgttttac catcaataga aagcgcatcg ttcacattca accaggtgag
    15961 tttctcttgc gacgttttct catcaatagt cgagaacagc cccgtcatct gattagattt
    16021 ctcggaccac atcacagcga tacgttgcga gccacagtca tgcggtttgc acgcgctcat
    16081 cacctgatac gtctcatctc ccaatgtcac ggtttgtgcg ggagtataag taccgccttt
    16141 catcacccag gcaggcagct tatgcccttg caccatctga ttaaacgccg ctttggtggt
    16201 ttcgcccttt gcaagactgc taatggttaa atcatcctgc gccattgcac tggtggcgat
    16261 gaccagagcg gcgactgtcg ttattgcctt aaacatcatt cctcccgggc ttatcctgcc
    16321 catgtgatta actccctaaa gcctggcgat aacacgataa tttgcaaatc agatatttcc
    16381 aatatcagca tttgttatca atttattctg cgctaaggca actgcctgat aaggtgttag
    16441 agttataaaa cccttcgcat aaaggagttg tacaccgtgc ctggtttgaa gattacgctt
    16501 ttgcagcaac cactggtgtg gatggatggt cctgccaacc tgcgccattt tgatcgtcaa
    16561 ctggaaggta ttaccgggcg cgatgtgatc gttctaccgg agatgtttac cagcggcttt
    16621 gccatggaag cggcagcttc gtcgctagca caagatgacg tagtgaactg gatgaccgct
    16681 aaagcgcagc agtgcaatgc gctgattgca ggcagtgttg cattacaaac ggagtctggt
    16741 tcggttaacc gctttttgct ggttgagccg ggcggcacgg tacattttta tgataagcgt
    16801 catctgttcc gcatggcaga tgagcatctg cattataaag cgggcaatga gcgagtgatt
    16861 gtggaatggc gcggctggcg tattttgccg ctggtgtgct acgacttacg ttttccggtg
    16921 tggtcgcgca atctcaacga ttatgacctc gccctgtacg tcgccaactg gcctgctccg
    16981 cgctctctgc actggcaggc attgctgaca gcccgcgcga ttgagaatca agcgtatgtg
    17041 gcgggatgca atcgcgtcgg cagcgatggc aacggctgcc attatcgcgg tgacagccgg
    17101 gtgattaatc cgcaaggcga gattatcgct actgccgacg cgcatcaggc aacgcgcatt
    17161 gatgcggagc tgtcgatgat ggcattgcgg gaatatagag aaaagtttcc ggcatggcgg
    17221 gatgcggatg agtttaggtt gtggtgagtg ggggttgtgt ttagggctgg ggagagtcag
    17281 ggtaagacta cgaaagcccg ctc
//
LOCUS       DAZKGF010000104        17288 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZKGF010000104 DAZKGF010000000
VERSION     DAZKGF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.104"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            113..202
                     /locus_tag="ACHOQV_003240"
                     /pseudo
     CDS             113..202
                     /locus_tag="ACHOQV_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            230..973
                     /gene="yciC"
                     /locus_tag="ACHOQV_003241"
     CDS             230..973
                     /gene="yciC"
                     /locus_tag="ACHOQV_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFP9631567.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            1003..1542
                     /gene="yciB"
                     /locus_tag="ACHOQV_003242"
     CDS             1003..1542
                     /gene="yciB"
                     /locus_tag="ACHOQV_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFP9631568.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            1647..2045
                     /gene="yciA"
                     /locus_tag="ACHOQV_003243"
     CDS             1647..2045
                     /gene="yciA"
                     /locus_tag="ACHOQV_003243"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFP9631569.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(2085..2804)
                     /gene="tonB"
                     /locus_tag="ACHOQV_003244"
     CDS             complement(2085..2804)
                     /gene="tonB"
                     /locus_tag="ACHOQV_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFP9631570.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            3028..3324
                     /gene="yciI"
                     /locus_tag="ACHOQV_003245"
     CDS             3028..3324
                     /gene="yciI"
                     /locus_tag="ACHOQV_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFP9631571.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            3625..4878
                     /gene="kch"
                     /locus_tag="ACHOQV_003246"
     CDS             3625..4878
                     /gene="kch"
                     /locus_tag="ACHOQV_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFP9631572.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(4933..5106)
                     /gene="yciY"
                     /locus_tag="ACHOQV_003247"
     CDS             complement(4933..5106)
                     /gene="yciY"
                     /locus_tag="ACHOQV_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFP9631573.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            5249..6709
                     /gene="cls"
                     /locus_tag="ACHOQV_003248"
     CDS             5249..6709
                     /gene="cls"
                     /locus_tag="ACHOQV_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9631574.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            6744..7073
                     /gene="yciU"
                     /locus_tag="ACHOQV_003249"
     CDS             6744..7073
                     /gene="yciU"
                     /locus_tag="ACHOQV_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFP9631575.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(7126..8130)
                     /gene="oppF"
                     /locus_tag="ACHOQV_003250"
     CDS             complement(7126..8130)
                     /gene="oppF"
                     /locus_tag="ACHOQV_003250"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFP9631576.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(8127..9140)
                     /gene="oppD"
                     /locus_tag="ACHOQV_003251"
     CDS             complement(8127..9140)
                     /gene="oppD"
                     /locus_tag="ACHOQV_003251"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFP9631577.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNRLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(9152..10060)
                     /gene="oppC"
                     /locus_tag="ACHOQV_003252"
     CDS             complement(9152..10060)
                     /gene="oppC"
                     /locus_tag="ACHOQV_003252"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFP9631578.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(10075..10995)
                     /gene="oppB"
                     /locus_tag="ACHOQV_003253"
     CDS             complement(10075..10995)
                     /gene="oppB"
                     /locus_tag="ACHOQV_003253"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFP9631579.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(11081..12712)
                     /gene="oppA"
                     /locus_tag="ACHOQV_003254"
     CDS             complement(11081..12712)
                     /gene="oppA"
                     /locus_tag="ACHOQV_003254"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFP9631580.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIITNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKATDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            12996..13292
                     /locus_tag="ACHOQV_003255"
     CDS             12996..13292
                     /locus_tag="ACHOQV_003255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631581.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSLFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(13450..14097)
                     /gene="ychE"
                     /locus_tag="ACHOQV_003256"
     CDS             complement(13450..14097)
                     /gene="ychE"
                     /locus_tag="ACHOQV_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFP9631582.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(14128..14181)
                     /gene="ychE"
                     /locus_tag="ACHOQV_003257"
     CDS             complement(14128..14181)
                     /gene="ychE"
                     /locus_tag="ACHOQV_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFP9631583.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            14574..17249
                     /gene="adhE"
                     /locus_tag="ACHOQV_003258"
                     /gene_synonym="adhC"
     CDS             14574..17249
                     /gene="adhE"
                     /locus_tag="ACHOQV_003258"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP9631584.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVAYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         4402 a         4253 c         4087 g         4546 t
ORIGIN      
        1 gcattgagaa tcctggttaa tgtgatttaa atcaattttt aaatattttc gctacgcaaa
       61 tattaactcg atcgccactt gccgcgattt gcccacgtct gaaggccagc aattacctcc
      121 gctttacaaa gatatgcttt tccatacaag acctgatgct gccacccgag gcttaaccag
      181 ggtacaatta cccgctaatt aacacctgca taaaactcaa ggagagtgca tgtctatcac
      241 ggcgcagtcc gtataccgtg acaccggaaa tttcttccgt aatcaattta tgaccattct
      301 gttggtatcg ttgctatgtg cgtttatcac agtggtgtta gggcatgttt tctcacccag
      361 tgatgcacag cttgcgcagc tcaatgacgg cgtgcccgtt agcggcagta gtgggttgtt
      421 cgacctggtt cagaatatgt caccggaaca gcaacaaatt ttgctgcagg cttcagcggc
      481 gtccactttt tcaggattaa tcggtaacgc cattctcgcc ggaggcgtaa tattaattat
      541 ccagctggtg tctgcgggtc agagagtcag tgcgctgcga gctattggtg ccagtgcgcc
      601 gatattgcca aagttattta ttctgatttt tctgactacc cttttagtac agattggcat
      661 catgctggtg gtcgttccgg gaattatcat ggccatttta ctggccctgg caccggtgat
      721 gttggtacag gacaaaatgg gcgtttttgc ctcgatgcgt agcagtatgc ggctgacttg
      781 ggcgaatatg cgtctggtgg cacccgcagt actgagctgg ttgctggcaa agacactgtt
      841 gctgcttttt gcctcttctt ttgccgcatt aaccccggaa attggtgccg tactggcgaa
      901 caccttgagc aacctgattt cagccgtact gctcatctat ctgttccgcc tgtatatgtt
      961 gattcgccaa taaccttaag catttgatta cggaatcgta aaatgaagca gtttcttgat
     1021 tttttaccgc tggttgtctt tttcgcgttt tacaagattt atgacatcta tgcggctact
     1081 gcggcgctga tcgtcgccac ggcgattgtg cttatatata gctgggttcg ctttcgtaag
     1141 gttgagaaga tggccctgat cacttttgtt ctggtggtcg tcttcggtgg cttgaccctg
     1201 ttcttccaca atgatgagtt tattaaatgg aaggttactg tcatttatgc cctgtttgcg
     1261 ggtgccctgt tagtcagcca atgggtgatg aaaaagccgc taattcagcg gatgctgggt
     1321 aaagaactca cgctgccgca accggtatgg tcgaagctga atctggcctg ggctgttttc
     1381 tttatccttt gcggtctggc aaacatctac atcgcattct ggctgccgca aaatatttgg
     1441 gtcaacttta aagtctttgg cctgaccgcc cttaccttaa tcttcacatt gttaagcggt
     1501 atctatatct accgccacat gccgcaggaa gataaatcct aaccagactg ccagtccttc
     1561 ggggctggcg ctgtatctcc tctgttctcc tcaggaaaat catagtagca tcgcgcctgt
     1621 gattttcctt ttaagtcggt tttaccatgt ctacaacaca taacgtccct cagggcgatc
     1681 ttgttttacg tactttagcc atgcccgccg ataccaatgc caatggtgac atctttggtg
     1741 gttggttaat gtcacaaatg gatattggcg gcgctattct ggcgaaagaa attgcccacg
     1801 gtcgcgtagt gaccgtgcgg gttgaaggaa tgactttctt acgaccggtt gcggtcggcg
     1861 atgtggtgtg ctgctatgca cgctgtgtcc agaaagggac gacatcggtt agcattaata
     1921 ttgaagtgtg ggtgaaaaaa gtcgcgtctg aacccatcgg gcaacgctat aaagcgacag
     1981 aagcattatt taagtatgtc gcggttgatc ctgaaggaaa acctcgcgcc ttacctgttg
     2041 agtaataatc aaaagcctcc ggtcggaggc ttttgacttt ctgcttactg aatttcggtg
     2101 gtgccgttaa ttttaaacag gatgttcacc acaatcccac tgcctggctt acccggctca
     2161 taacgccatc tgcgcatcgc atttttcacc tcacgctcaa acatgttcgc aggcttggct
     2221 gagaggattt gtacgttatc cacgcgacca tccggcgtga catcaaattt aactttaacc
     2281 tgcccttcaa tgcgcaatgc ctgtgctcgt gccggatact gcggctgatt gcggcttaat
     2341 gcgcgtggtc ctgaagccac actggtaacc ggcttgctgg ttgcagccgt tgccgtactt
     2401 gatgtagggc gcgcgggtgc agtattttca aacggtgatg ccggacgcga ctctacgggt
     2461 ttgacatcgc gttttggctg ctcctgtacc tttttcaccg gcttcggttt tggcttaggt
     2521 ttcggcttcg gcttttcaat gaccaccggt gcttcttttg gcggttcggg gatcggctca
     2581 ggttccggtt ctggctctac caccggctcc ggtggcggct gaacggcttg tggcggttcg
     2641 agatccgcag gcgcaaccat cgtgacagaa attggctgcg caggcgcagg tagttcaata
     2701 acctgatgta ccgaggtata gagcagaccc gccacaacag caccatgaat gcagaccgaa
     2761 agtaacgtcg gccaggggaa gcggcgaggt aaatcaaggg tcattgaagt cataatcatt
     2821 tcagtagaaa aaccaggcct cgattttaaa tgcaaatagc aatcatattc aataaggcaa
     2881 gacgatcagg atcagtgaaa atgtcatttt tggcgtaatt gaccttactt tttaaatggt
     2941 cttaacaaag acatcatctt tccgttgcag atcttcgccc tttcaaataa cgtactttac
     3001 aactttcccg aacaaggagt tgtgcccgtg ttgtatgtta tttacgctca agacaaagct
     3061 gatagcctcg aaaaacgcct ttccgttcgt ccggcacatt tagcacgttt acagttactg
     3121 catgatgaag gtcgattgtt gaccgccggt ccaatgccag cagtagacag taacgatcca
     3181 ggggctgcgg gatttactgg ctcaaccgtt attgctgaat ttgaatcgct ggaagcagct
     3241 caggcctggg ccgatgctga cccgtacgta gcggcaggag tctatgaaca cgtttcagtg
     3301 aaaccattta agaaagtgtt ctgaatagaa attcaatatt taagggcacg gttttagcaa
     3361 ccgtgttctt gtttggcctt tccttatagg tcagtcattg catttaccac cacaagcaga
     3421 taataacttt atctgtattt ctttatatga tgtgagcata ttcaggaaaa atgccctttc
     3481 ctgacgcaga gttgctcact caaaaccttt gattactgcg gataaagtca tctactattg
     3541 tttaacacat tcacgcgctg catgtatgtt cagaaaatgt gcacgctaag ataattaaac
     3601 aaaattgtgg ttacagaggt atcagtgagt cactgggcta cattcaaaca aacagcaact
     3661 aatttatggg tgaccctacg gcacgacatt ctcgcgctgg ccgtcttttt aaatggattg
     3721 cttattttta aaacaatcta tggtatgtcg gtcaatttgc tagatatttt ccatatcaaa
     3781 gcattttcag agttggatct ctccttgctg gcaaacgccc cactctttat gctcggcgtc
     3841 tttcttgtcc tgaactccat tggcttactg ttccgggcaa agctcgcatg ggcaatcagt
     3901 atcattttgt tgttgatagc gctaatttac accctgcatt tttatccctg gctgaaattt
     3961 agtattggat tttgcatttt tacgctggtg tttttgctga tactgcgcaa agacttctcc
     4021 cacagtagcg ccgcagccgg gacaattttc gcatttatta gtttcacgac gttactgttt
     4081 tactccacct acggtgcgct ttatttaagc gaaggtttta atccgcgaat agaaagtttg
     4141 atgaccgcgt tctatttttc gatagaaacc atgtcaaccg tcggctatgg cgatattgtc
     4201 cctgtttctg aatcagcacg attgttcact atttcggtca ttatttccgg cattaccgtt
     4261 tttgccacat ctatgacctc aatttttggt ccgcttatcc gcgggggatt caacaaactt
     4321 gtaaaaggaa acaatcatac aatgcatcgt aaagatcatt ttattgtttg cggacattcg
     4381 attctcgcca tcaatacgat cctgcaactg aatcaacgcg gacaaaacgt aacggttatc
     4441 agcaacttgc ctgaagatga tatcaagcaa cttgagcaac gcttaggcga taacgctgat
     4501 gttatccccg gtgacagtaa tgacagttca gtattaaaga aagctggaat tgatcgatgc
     4561 cgggccattc tggcgctgag tgataacgat gcagataacg cgtttgttgt actctcggca
     4621 aaagatatga gcagtgatgt caaaacagtt ctcgccgtca gtgatagcaa aaacctgaat
     4681 aagattaaga tggtacatcc ggatatcatt ctctcgccgc aactgtttgg cagcgaaatt
     4741 ctggcgcgag ttttaaatgg tgaagagatt aataatgata tgctcgtttc aatgttgttg
     4801 aactccggtc atggcatttt cagcgataac gatgaacaag aaacgaaagc tgacagtaaa
     4861 gaatcggcgc aaaaatagag tttaaagagg gaagagtaaa cttattgggc accgcaatgg
     4921 tgcccagtac atttagatat tgtagatcgc aaacaataac gagttacgct gtttgtttag
     4981 aatgcacttc ctgatgctgt ggatacgcat atttcggcgc gactgcccct caagccaacg
     5041 cgatttacgt cggctagcct gacgctgcat gcgccagcgc cccacttccg ttctactccg
     5101 cttcatgtta actactctat gcaataacag aatggtcatt atacccccgc ccccgacgct
     5161 gaccagtgct tttcccgcgt ttttatttgc cagatgaatc catatgcgta aactcataac
     5221 aatgcgcttt caaaaggatt tctaatctat gacaaccgtt tatacgttgg tgagttggtt
     5281 ggccattctg ggatactggt tgctcattgc aggcgtaact ttacgcattc taatgaaacg
     5341 acgcgcagtt ccctccgcga tggcctggct gttgattatt tacattctgc cgttagtcgg
     5401 aattattgcc tatcttgccg ttggcgagct ccatttaggc aaacgccgcg ctgagcgcgc
     5461 cagagcgatg tggccttcca ccgcaaaatg gcttaacgac cttaaagcct gtaagcatat
     5521 cttcgccgaa gaaaatagca gtgtcgctgc gccattattc aagctttgcg agcgtcgtca
     5581 ggggatcgct ggggtcaaag ggaatcagct gcaactgatg accgagtcag atgatgtgat
     5641 gcaggcgtta atccgcgaca tccagctcgc tcgccataat attgagatgg tgttttatat
     5701 ctggcagccc ggcggcatgg cggaccaggt ggctgaatca ttaatggcgg ctgcgcgacg
     5761 cggcattcat tgccgattga tgctcgactc cgccgggagt gtggcttttt tccgcagccc
     5821 gtggcccgag ctaatgcgta atgccggtat tgaagttgtc gaagccttaa aggtcaatct
     5881 gatgcgtgtg tttttacgcc gtatggacct gcgccaacat cgcaagatga tcatgatcga
     5941 taattacatc gcgtacaccg gcagcatgaa tatggtcgac cctcgctact tcaaacaaga
     6001 tgcgggcgta gggcaatgga ttgatctgat ggcgcgtatg gaaggtccca tcgctaccgc
     6061 gatggggatt atttattcct gcgactggga gattgaaacc ggaaaacgta ttctgccgcc
     6121 accaccagat gtcaatatta tgccgtttga gcaggccagc ggtcacacca ttcacacaat
     6181 tgcttctggc cccggctttc cggaagatct cattcaccag gcattattga ctgctgctta
     6241 ttcggcgcgt gaatatttga tcatgacaac gccctacttt gtgcccagcg atgatttact
     6301 tcatgcgatt tgcacggcgg cgcagcgcgg ggtggatgtc agtattatcc ttccgcgaaa
     6361 aaatgactcg atgctggtcg gctgggccag tcgcgcattc tttacggaac tgctggctgc
     6421 tggggttaaa atttatcagt ttgaaggcgg gttactgcat accaagagcg tgctggtcga
     6481 tggtgaacta agtctggttg gcaccgttaa ccttgatatg cggagtctgt ggctaaattt
     6541 cgagattacc ctggcaatcg acgataaagg ttttggtgca gaccttgccg ccgttcagga
     6601 cgattatatt tcgcgttcac gtctgctcga tgcccgttta tggctaaaac gtccattatg
     6661 gcaacgtgtc gccgagcgac tgttttactt cttcagcccg ttgctgtaaa acgtcgccaa
     6721 cagaggttaa acaggtagtg actatggata tggatctaaa caatcgcctg actgaagatg
     6781 aaacgcttga gcaggcctac gatatttttc tcgaactggc tgccgacaat ctcgatccag
     6841 ctgatgttct gctgttcaat cttcagtttg aagagcgcgg cggcgcggag ttattcgatc
     6901 cggcagaaga ctggcaggaa catgttgatt tcgatttgaa cccggacttt tttgccgaag
     6961 tggtgattgg tctggcggac agtgaagatg gtgaaatcaa cgatgttttc gcgcgcattc
     7021 tgttatgccg cgaaaaagat cacaaactgt gccatatcat ctggcgggaa taaataatcg
     7081 cttttggcgt gaataagggc tgacaactgt cagcccttat tgcttttaaa gcggatcgac
     7141 tttcaggcaa gaaacggcgt gtctgaaact cccctccagc acaggacgtg ttttggcaca
     7201 ctctggaccg gcaatcgggc aacgggtacg gaaaacacaa ccggaaggcg ggttgatcgg
     7261 cgacggtaat tccccttcca gtaactggat ggttttgttc ttctccagat ccggatcagg
     7321 tatggggact gccgacatca atgccctggt gtaaggatgt agcggattgt gatagacctc
     7381 atcataggtc cccagttcta ccgcatggcc gagatacatc accagcacac gatcggaaat
     7441 gtgttttacc acggccaggt catgagcgat aaaaattaat gacaatccca tctcgcgttg
     7501 cagctgctgg agcaggttga ccacctgcgc ctgaattgac acgtccagcg ccgacaccgg
     7561 ctcatcacag ataatcagct tcggttcaag aataagagca cgagcaatcc cgatacgctg
     7621 gcactgccca ccagagaact catgcggata gcggttaatc aggttaggca ataacccgac
     7681 tttcagcatc atcgccttca cgcgctcgcg aacttcctgg cgtgacattt tcggatgata
     7741 agtacgcagt ggttcagcga tgatctcgcc gatggtcata cgcgggttaa gcgatgccaa
     7801 tggatcctgg aaaatcatct gaatatcact gcgaacggca cgccattcat cgggcttcat
     7861 gcccagcaac tctttaccta accaggcaac atggccgtcg gtcgccttga ccaaaccaat
     7921 gatggcgcga gcaaaggtgg acttaccgca tcccgattcc cctaccacac ctaatgtttc
     7981 cccttcatac aggcgaagag tgacaccatc gacggctttg agcgttttcg gcggttgcca
     8041 gaaccactgt ttgccatctt tgatttcaaa gtgcactttc agatcggcga tttcgaggag
     8101 gacttttctt ccttcggtta cagcattcat aacagttcct ccaccggttt aaagcaagca
     8161 cgcagacggc caggcgtaaa ctcttccagc ggcggtgcgc tactacaaat ttccatcgca
     8221 tgcggacaac gtggctggaa cgggcaacct ttcggtaatc gcagcaggtt tggcggatta
     8281 ccagggatgg tcaacattgt ttcaccttcc gcatcgagac gcggcaccgc gttgagcaaa
     8341 ccgatagaat aaggatgaac gggttgataa aagacatcgc gcgcgttgcc atattccatc
     8401 gtgcgcccgg cgtacattac cagcactttg tcgcagatcc ccgccaccac gccaagatcg
     8461 tgggtgatca taatgatggc ggtattaaat tcccgcttca gttcatttaa tagcgtcatg
     8521 atctgcgcct gtacggtgac gtccagcgca gtagtgggtt catccgcaat cagcagctta
     8581 ggtcgacata gcaatgccat cgcaatcatg actcgctgac gcatgccacc agaaaattca
     8641 tgcgggtaca ttttcatgcg tttacgtgct tccggcattt ttaccgcatc gagcatccgc
     8701 accgactctt caaacgcctc agctttgctc atgttcttat gcagcatcag cacttccatc
     8761 aactgctcac cgacgcgcat atagggattc aacgaagtca ttgggtcctg aaaaatcatt
     8821 gagatttgtt cagcgcgcag tctattgagt tcacgctctg gcaaattgag gatttcacgc
     8881 ccattgaagg ttgccgatcc gccaatacgt ccgttggcag ccagcaggcc catcaacgca
     8941 aatgcagttt gcgatttacc cgaaccagac tcacctacaa tgcccagcgt ttctccggca
     9001 cgtagggaaa aattcaaatc attgaccgcc gtgacgtcgc cgtccggggt actaaaggtg
     9061 acacgcaaat ctttcacgtt cagcagtgcg tcagcctgtt gttgtgcgag cggcacagtt
     9121 gcagtttcaa ttacgctcat ggctgcactc cttaacgatc tttcgggtcg agggcatcac
     9181 gcaagccatc gcctataaag ttgaaacaaa atagcgtcac cacgaggaat cccgctggga
     9241 acaacagtaa ccatggagag acttccatcg agttcgcgcc atcactcagc aatgcgcccc
     9301 agctgcttaa cggctcttgc gtccccaacc ccaggaagct aaggaaagat tcaaagagga
     9361 tcatgctggg caccagaagt gatgcgtaga ccaccaccac accgagtaca ttcggcacaa
     9421 tgtggcgaat aacaatgccc gacgtcgata caccgccaac ttgtgccgcc tcaataaact
     9481 ctttgcgctt cagactcagg gtttgcccac gcacaatacg agccatatcc agccaggaaa
     9541 ccatgccaat cgccacgaaa atcagcagga tgttttgacc gaaaaaggtc accagcaaaa
     9601 tgacgaagaa catgaatggg aaggagttga ggatttccag cagacgcatc attaccgaat
     9661 ccactttacc gcccagataa ccggaaagcg aaccataaag tgtccccacg accactgcca
     9721 ccagtgccgc agcaacaccg accatgagtg agatacgccc gccaatcgca acacgcacaa
     9781 gcaggtcgcg accggatgag tcagtaccaa agtagtgacc ggactccata tccggggcgc
     9841 tggacatcat cgcccagtca gtatcgtcat aggcaaactg cgaaagcatc ggtgccagga
     9901 ttacaaataa cgcgattagc accagcacta tcagactggc aaccgccgca cggttatgca
     9961 taaaacgtcg acgtgcgtcc tgccacaagc tgcgcccttc gacctccagc ttttcactga
    10021 aattttccag cgtctcgctg tttttcttac ttaacatcat cgcgagctcc agtatcagta
    10081 acggattttc gggtcgataa ccgcatatag cacatcgaca atggcattaa acaaaatggt
    10141 taacgcacca accaggatag tcaggcttaa cactaaggaa tagtcacggt tcaatgcgcc
    10201 attaacgaac agttgcccaa tccccggcaa accataaatg gtttcgatga ccatagaacc
    10261 ggtaataatg ccgacaaatg cggggcccat ataggagagc acgggtaaca gagcaggttt
    10321 taatgcgtgg cgtaaaatga tccgccgcat aggtaaccct ttcgcccggg cagtacgaat
    10381 aaagttggag tgtaatactt caatcataga gccacgggta atacgcgcaa tactggcgat
    10441 ataggcgagt gacaacgcca ccattggcag tatcatgaat ttaagcgcac caccattcca
    10501 gccaccgccc ggcagccaat gcaaaatgat cgcaaatatc atgactaata atggcgcaac
    10561 cacaaaactg gggataacaa ccccggtcat tgccagcccc atcacggtat agtcccattt
    10621 ggtgttttgt tttaatgcgg caataacgcc agcactaaca cccagtatta ccgcaaggaa
    10681 aaatgctgcg gctcccagtt tggcagaaac gggaaaactg gatgcaacca ggtcgttgac
    10741 cgaataatct ttatatttaa acgatggacc gaaatcgcca tgcgccagtt gtttcaggta
    10801 gctgaaatac tgtgtcatga tcggatcatt aagatgatat ttcgcttcga tattggccat
    10861 cacttctggc ggtaaagtac gttcgccggt aaaagggctt cccggcgcga ggcgcatcat
    10921 gaagaacgaa atagtaataa gaataaatag cgtcggaatc gcttccagac agcgacgtag
    10981 aataaatttt aacattgccc taccttctgg cctgtgcctt ctgtaatgcg ataaaaatca
    11041 gacaccgtgg agcagtacac gcctgcccca cgtattgcca ttagtgcttc acaatgtaca
    11101 tattccgggt gtaggtatta tccagcggat ctttgccggt atagccacca acccacggtt
    11161 tcaccagacg cgcattcacg tagtaataaa caggaacaat ggccgaatcc ttatccagct
    11221 gttgttctgc tttagtgtac agagctgtgc gctgcgcctc gtcagtcgct ttcagcgttt
    11281 ccgccataat gctgtcaaag gccgggctct tataatgcgc ggtattcatt gagctgttcg
    11341 acagcatggt gttcaggaag gaagttggtt cgttgtagtc tgcacaccag cctgcacggg
    11401 ccacatcaaa agtgccctgg tgacgggtgt cgaggaacgt tttccactcc tggttaacca
    11461 gtttgacgtt tacaccaatg tttttcttcc acaatgaaga ggcagcaatc gccagctttt
    11521 tatgcagatc ggaggtgtta tacaacaggt tgatggtcaa cggtttgtct gcggtataac
    11581 ccgcttcagc cagcagtttt ttcgcttctt cgttacgttt ttcctggctc cagccaaacc
    11641 attccggctg agtcaatttt gcgccatcag tatacggtgg agtgtaacca taggcgggca
    11701 tgtcgccctg cgctttcact ttattaacaa tgatatcgcg gtccataccc agtttcagtg
    11761 cggtacgcac acgtacatcg ttgaatggcg gtttctggtt gttaatttcg taataataag
    11821 tgcacaggta tggatcaacg tgaacttcgt ccgggatctc ttttttcagc ttctggaaca
    11881 attcgatcgg catgttgtta tacgtcatgt cgatttcacc actacggtag cggttgacat
    11941 cggtaacttc agaagcaata ggcaaatagg ttacctggtt aataacggtt ttcgcgttgt
    12001 tccagtaggt tgggctgcgt tcaagaacga ttcgttcgtt tacgacccaa tcttttaagg
    12061 tataggcacc gttggtgatg atattaccag gctgggtcca tttttcgccg aatttctcga
    12121 tagcggcttt tggcaccggt gaagttgatg ggtgaacaag taatttatag aagtacggaa
    12181 caggttcact taaggtgact tctaatgtgt gatcatcaat tgctttcacg ccgagatcgg
    12241 taatcggttt tttcccttca agaatttcat caataccggc gatatgccca tattgcagat
    12301 aactggcata cggagaagcg gtgtttggat caacagaacg ttgccagcta tacacaaagt
    12361 cttgtgctgt gactggcgtg ccatcagacc atttcgcatc tttacgcaaa tggaaggtcc
    12421 agactttcgc gtctttatta tcccaggatt cagcgacgcc aggtgccgga tgaccgtcaa
    12481 gatcgctgac cagtaagcct tcaaacaggt ctcggctgat attagactcc ggaacacctt
    12541 caattttatg cggatctaat gactgaactt ctgaaccatt gttacgtacc agcgtttgtt
    12601 tttccgccag tgtgacgcct gcgggtacat cagctgccag cgcgacattc cctgccatta
    12661 gtgcagccag aacgccagct gctactaaac ttctcttggt gatgttggtc attgtttttt
    12721 ggactccctc attataatta ctggtattaa ccagcatgtg taatcccgga ttgggaccct
    12781 gcacaggctc ggagactttc tgctgtcagg ttactttatc gttacggatt gctatcaccg
    12841 actttattta ttctggtgat tcagacgatc accttatgtc gattcgtact gtgtctcccc
    12901 tgtcgagaca cattattctt tcgcgacgct ctttaattgt taattattct cattaccaat
    12961 aaagaatcgc ctggcggttg cccggaaagt accaaatgcg cttcttactc gccaatacat
    13021 tttgcaaaat tgttaagcaa ttctctttta ttgaccgcat cagaatctat agcttgcatc
    13081 catgtcttaa caaacatctt tcaattcaat caaatagaga ttggttaagc atgtctctct
    13141 tttctgatta cagttccagt agtgaaatgc acaataattt aacaattgat tattatttag
    13201 cactttcctc taccaaaggc agtggaatta ctaatatcat ttcaattatt cttcagcaag
    13261 cccaggatta tgatgtggcg aaaataacat gatggtgagg agaagcagtt aaggaaatcc
    13321 ttataactaa ctgtaatttg agaagaatat tttcacccat tccttttgct tttgataact
    13381 gcgcgggaac aaagtaaaaa taatgacgaa gtaaacggag catgacagct ccgtttcatt
    13441 tgaaaggaat taattaagca ggccggggaa aatcccctta ataccagtaa cgataaattc
    13501 aatccccaat gccatcagca atagccccat aatacgcgta atcacgttga tgccggtctg
    13561 gcgtaaaacc cgtaccagcc acggtgccat gcggaacaat ccccagcaac ataaagcgaa
    13621 caatgcaata gccacaaaga aaccaaacag atagctaatg ctgtgataac gcgtccccca
    13681 gacgatggta gaactgatcg cccctggccc cgccatcaac ggcaacgcca atggcaccac
    13741 accaatgctt tcacgcaccg cggtttctga tttttcttgc ttgttctgtt tatcctcgcc
    13801 aagcttgccg ctgatcatcg acatcgctat cgtcaccacc aggatgccac cggcgatacg
    13861 gaatgaatca attgatatac caaaaagttg tagaatcgtg tcgccgagaa aaagcgagat
    13921 ccacaagata atggccacag acaggttggc tgtaaggttc gttttgtttc gcgctgccgc
    13981 tgtctgataa ctggtcatgc tgataaagac gggaataatc cctaccgggt tgaccagcgc
    14041 aaataacccg atgaaaaatt tgaagtaaac gggaaaatca aaaagggtct gaatcacggt
    14101 tagctccgaa gcaaaagccg gataatgtta gccataaata aggttaaaaa gacgcgctga
    14161 caatacgcct tttgacagca tttttcacct cctaactact taaaattgct atcattcgtt
    14221 attgttatct ggttgtgcaa aacatgctaa tgtagccaac aaatcatact acaatttatt
    14281 aactgttagc tataatggcg aaaagcgatg ctgaaaggtg tcagctttgc aaaaatttga
    14341 tttggatcac gtaatcagta cccagaagtg agtaatcttg cttacgccac ctggaagtga
    14401 cgcattagag ataataactc taatgtttaa actcttttag taaatcacag tgagtgtgag
    14461 cgcgagtaag cttttgattt tcatcggtta agcaaatcat caccgcactg actatactct
    14521 cgtattcgag cagatgattt actaaaaaag tttaacatta tcaggagagc attatggctg
    14581 ttactaatgt cgctgaactt aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat
    14641 atgccagttt cactcaagag caagtagaca aaatcttccg cgccgccgct ctggctgctg
    14701 cagatgctcg aatcccactc gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg
    14761 aagataaagt gatcaaaaac cactttgctt ctgaatatat ctataacgcc tataaagatg
    14821 aaaaaacctg tggtgttctg tctgaagacg acacttttgg taccatcact atcgctgaac
    14881 caatcggtat tatttgcggt atcgttccga ccactaaccc gacttcaact gctatcttca
    14941 aatcgctgat cagcctgaag acccgtaacg ccattatctt ctccccgcac ccgcgtgcaa
    15001 aagatgcaac caacaaagcg gctgatatcg ttctgcaggc tgctatcgct gccggtgctc
    15061 cgaaagatct gatcggctgg atcgatcaac cttctgttga actgtctaac gcactgatgc
    15121 accacccaga catcaacctg atcctcgcga ctggtggtcc gggcatggtt aaagccgcat
    15181 acagctccgg taaaccagct attggtgtag gcgcgggtaa cacgccagtt gttatcgatg
    15241 aaactgctga tatcaaacgt gcagttgcat ctgtgctgat gtccaaaacc ttcgacaacg
    15301 gcgtaatctg tgcttctgaa cagtctgttg ttgttgttga ctctgtttat gacgcagtac
    15361 gtgaacgttt cgcaacccac ggcggctatc tgttgcaggg taaagagctg aaagctgttc
    15421 aggatgttat cctgaaaaac ggtgcgctga acgcggctat cgttggtcag ccagcctata
    15481 aaattgctga actggcaggc ttctctgtac cagaaaatac caagattctg atcggtgaag
    15541 tgaccgttgt tgatgaaagc gaaccgttcg cacatgaaaa actgtccccg actctggcaa
    15601 tgtaccgtgc taaagatttc gaagacgcgg tagaaaaagc agagaaactg gttgctatgg
    15661 gcggcatcgg tcatacctct tgcctgtaca ctgaccagga taaccaaccg gctcgcgttg
    15721 cctacttcgg tcagaaaatg aaaacggccc gtattctgat caacaccccg gcgtctcagg
    15781 gtggtatcgg tgacctgtat aacttcaaac tcgcaccttc cctgactctg ggttgtggtt
    15841 cctggggtgg taactccatc tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa
    15901 ccgttgctaa gcgagctgaa aacatgttgt ggcacaaact tccgaaatct atctacttcc
    15961 gccgtggctc tctgccaatc gcgctggatg aagtgattac tgatggccac aaacgtgcgc
    16021 tcatcgtgac tgaccgcttc ctgttcaaca atggttatgc tgatcagatc acttccgtac
    16081 tgaaagcagc aggcgttgaa actgaagtct tcttcgaagt agaagctgac ccgaccctga
    16141 gcatcgttcg taaaggtgca gaactggcaa actccttcaa accagacgtg attatcgcgc
    16201 tgggtggtgg ttccccgatg gatgccgcga agatcatgtg ggtgatgtac gagcatccag
    16261 aaactcactt cgaagaactg gcgctgcgct ttatggatat ccgtaaacgt atctacaagt
    16321 tcccgaaaat gggcgtgaaa gcgaaaatga tcgctgtcac caccacttct ggtacaggtt
    16381 ctgaagtcac tccgtttgcg gttgtaactg acgacgctac tggtcagaaa tatccgctgg
    16441 cagactatgc gctgaccccg gatatggcga ttgtcgatgc caacctggtt atggacatgc
    16501 cgaagtccct gtgtgctttc ggtggcctgg acgcagtaac tcacgccatg gaagcttatg
    16561 tttctgtact ggcatctgag ttctctgatg gtcaggcact gcaggcactg aaactgctga
    16621 aagaatatct gccagcgtcc taccacgaag gatctaaaaa tccggtagcg cgtgaacgtg
    16681 ttcacagtgc agcaactatc gcgggtatcg cgtttgcgaa cgccttcctg ggtgtatgtc
    16741 actcaatggc gcacaaactg ggttcccagt tccatattcc gcacggtctg gcaaacgccc
    16801 tgctgatttg taacgttatt cgctacaacg cgaatgacaa cccgaccaag cagactgcat
    16861 tcagccagta tgaccgtccg caggctcgcc gtcgttatgc tgaaattgct gaccacctgg
    16921 gtctgagcgc accgggcgac cgtactgctg ctaagatcga gaaactgctg gcatggctgg
    16981 aaacgctgaa agctgaactg ggtattccga aatctatccg tgaagctggc gttcaggaag
    17041 cagacttcct ggcgaacgtg gataaactgt ctgaagatgc attcgatgac cagtgcaccg
    17101 gcgctaaccc gcgttacccg ctgatctccg agctgaaaca gattctgctg gatacttact
    17161 acggtcgtga ttatgtagaa ggcgaaactg cagcgaagaa agaagccgct ccggctaaag
    17221 ctgagaaaaa agcgaaaaaa tccgcttaat cagtagcgct gtctggcaac ataaacggcc
    17281 ccttctgg
//
LOCUS       DAZKGF010000105        17275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZKGF010000105 DAZKGF010000000
VERSION     DAZKGF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.105"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..480
                     /locus_tag="ACHOQV_003259"
     CDS             <1..480
                     /locus_tag="ACHOQV_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP9631585.1"
                     /translation="LASFTLAGCQSNADDHAADVYQTDQLNTKQETKTVNIISILPAK
                     VAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGGAVGAAAGSMVN
                     DKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRIQPNTKCPEKS"
     gene            496..1035
                     /locus_tag="ACHOQV_003260"
     CDS             496..1035
                     /locus_tag="ACHOQV_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFP9631586.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1128..2705)
                     /gene="guaA"
                     /locus_tag="ACHOQV_003261"
     CDS             complement(1128..2705)
                     /gene="guaA"
                     /locus_tag="ACHOQV_003261"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9631587.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2774..4240)
                     /gene="guaB"
                     /locus_tag="ACHOQV_003262"
     CDS             complement(2774..4240)
                     /gene="guaB"
                     /locus_tag="ACHOQV_003262"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP9631588.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4402..5778
                     /gene="xseA"
                     /locus_tag="ACHOQV_003263"
     CDS             4402..5778
                     /gene="xseA"
                     /locus_tag="ACHOQV_003263"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP9631589.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDNAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQLKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANLRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     THQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLTTRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(5847..13898)
                     /locus_tag="ACHOQV_003264"
     CDS             complement(5847..13898)
                     /locus_tag="ACHOQV_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
                     /protein_id="HFP9631590.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPAKTHEVAFTAKPSEFSADVSVSKLLVTNPQDREGDIIAT
                     PRWENQTPPTVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLIAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLTLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKTTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQTSETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEDGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNYYWSSLAGMSGQHYAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGVNR
                     KNQTDAAWETHPVQIDGGSNVRKVDAHTYEGITDANGEASLTLTQPGGVGVKTHITAK
                     MRSDYTASDEKDVIFTVITSPDSDKARMWGHMLGIIEANNIFKRPRLADETDNELGAV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANVIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAQIAATTDHDVSTSSRAQAKVG
                     ETITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQPQGVGLATPLNIAPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVSSPDGTLTENNEVWSRVSQANTSSTSKG
                     GCGANMLPRRSQLNALYDANSGNAVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDMPFTLKRGDGYTRSNEKHIAGGGDALVAPVVINSGLADETSLNDTAAAYS
                     AMTGSDGTKILTITRPDTHGTKTSLTAALYSDTTKKATLDTIFTVVTSPDSDKAKMWG
                     HMPETVTAADGTVFKRPLLLKELSSTSGRTAIAEENEDWAQFTQTQAISTSSNGCGSE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADPTKFDSTNNVVKAKKGEEAVLRVTTKDAQ
                     GNPVGNTAFTLKRNTSVNRANVSTTTSIASLAVTDAWGNTQDDFLSTTLVIYGVTGAD
                     GITTFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETA
                     TGDDGLIYRRPLLRDENSATTSIGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDN
                     ELRAIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSG
                     GALLSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNS
                     VPNVPFILRREGSKNRQNAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFT
                     VKQDDSMGLVTNMYAQLYQPTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGI
                     AFKRPLLTAEHPAGQSTMANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEH
                     PNGAIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNP
                     HAVAASIEMTSTALDAEKTASNDGRPSATAAKGTAIRMTVIVRDSAGNLLPGANFNLI
                     RGTALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNE
                     TYGLATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRP
                     LLAAEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPGGTILTD
                     LGLPITAGSGNWWTYEMSTTDALTWYYGVINLKTGQSTTTINGYALMLCLTQPHSAPA
                     SLTLSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAK
                     SRSGISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGL
                     VTPFTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWS
                     ELTSTSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFV
                     TETGWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTS
                     TAFDAATQAAKVKKGEAMPVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGDVV
                     AMDDLIVQPLSGSAVTLSESGNTISGMTGADGTASFTLRQDNTPGYKTPLTVTLANYA
                     SATDTLDAIFTVPTSPNVSSAHFWGHMADTVVVNGKSLHRPLLTTELPSGANPVSSPI
                     INYENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSS
                     QQCGMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(14019..14996)
                     /locus_tag="ACHOQV_003265"
     CDS             complement(14019..14996)
                     /locus_tag="ACHOQV_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFP9631591.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEESAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADHVTLTSDLKGARPTGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELATKVTSYTLTDDDRGRYIGIEITPTT
                     QTGTPNVGTALHLYDISTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLAIPATNDEARTNLNGPARDGKEALTIPTNGDGVQGYKLHIIYKHK"
     gene            complement(15051..17228)
                     /gene="sinH"
                     /locus_tag="ACHOQV_003266"
     CDS             complement(15051..17228)
                     /gene="sinH"
                     /locus_tag="ACHOQV_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HFP9631592.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYIINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDEWLLGGNIFYDYDFTRGHRRLGLGGEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPSYPQLGGKVVFEQYYGDEVALFGTDNLEKDPYAVTLGLNYQPV
                     PLLTVGADYKAGTGDNSDVSINATLNYQFGVPLKDQLDSDKVKAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVTLWLKADATNEHPECVIKDTPEAAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDKAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPRLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNTYRFVLWRDSNKDGVFQQVEKLTDEEMAQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         4056 a         4603 c         4292 g         4324 t
ORIGIN      
        1 ttagcgtcat tcactctggc aggatgccag tcaaatgctg atgatcatgc cgccgatgtt
       61 tatcaaaccg atcaactgaa taccaaacaa gaaactaaaa ccgttaatat tatttccatt
      121 cttcccgcaa aagttgccgt agacaacgcc caaaataaac ggaacgcaca agccttcggc
      181 gcgcttattg gcgctgtcgc tggcggtgtt atcggccaca acgtcgggtc tggcagcaat
      241 tccggaacga cggcaggtgc agttggcggc ggagctgtag gcgcggcagc gggttctatg
      301 gtgaatgata aaaccttagt ggaaggtgtt tctttaacat ataaggaagg caccaaagtg
      361 tatacctcca cccaggtggg taaagagtgc cagtttacga caggtttagc cgttgttatt
      421 accacgacgt ataacgaaac acgtattcag ccaaatacca aatgtcctga aaagagctaa
      481 taatcaggag gagtcatgaa gaaagttttt ctttgcgcca tcttagcctc cttaagctat
      541 ccggctatcg cctcatcatt gcaggatcaa ctctcggctg tcgcagaagc ggaacagcaa
      601 ggtaaaaatg aagagcaaag gcagcatgac gaatgggtcg cggagcgcaa cagggaaatc
      661 cagcaagaga agcaacgtcg cgcaaacgcc caggccgcgg ctaataaaag agcggcaacg
      721 gcagcggcga ataagaaagc tcgtcaggat aaactggacg ccgaagccac tgcggacaaa
      781 aaacgcgatc aaagttatga agatgagcta cgcagcttag agattcagaa acaaaaactg
      841 gcgctggcga aagaagaagc ccgcgttaag cgagaaaacg aatttatcga tcaggaactg
      901 aagcacaaag ctgcgcaaac cgatgtggtg caatctgaag ctgacgcaaa caggaatatg
      961 actgaaggcg gtcgcgatct gatgaaaagc gtgggcaaag cagaagagaa taaatcagat
     1021 agctggttta actaaacgat gttagtaact tcaaacctat aattctttag gataaaaaac
     1081 cctctgtagt aacagagggt tttgttcatt catagtgcag ggtcaaatca ttcccactca
     1141 atagttgctg gtggcttgcc gctgatgtca tacaccacgc gggaaatacc attcacttca
     1201 ttgataatgc ggttggaaac gcgaccgagg aaatcgtacg gcagatgtgc ccagtgtgcg
     1261 gtcataaagt cgatggtttc gacagcacgc agagagacaa cccagtcata cttacgacca
     1321 tcgcccatta cgccaacgga acgtactggc aggaagacgg tgaatgcctg gctgactttg
     1381 tcgtacaggt ccgctttacg cagttcttca atgaagatgg cgtcagcacg gcgcagcagg
     1441 tcacaatact ctttcttcac ttctcccaga acacgaacgc caaggcctgg tcccgggaac
     1501 gggtgacggt acaacatgtc atacggtagg cccagctcca gaccaatctt acgcacttcg
     1561 tctttgaaca gctctttcag cggttcaacc aagcccatct tcatctcttt cggcaggcca
     1621 cccacgttgt ggtgagattt gatgacgtgt gctttaccgg ttgcagaagc agcagattcg
     1681 ataacgtcag ggtagatagt gccctgcgcc agccacttca cgtcttccag tttcagcgct
     1741 tcttcatcga atacttcaac gaaaacgcgc ccgatgattt tacgttttgc ttccggatca
     1801 ttttcgccag ccagcgctga caagaagcga tcttctgccg gtacgtgaac aatattcaga
     1861 ccaaagtgat cgccaaacat atccagaacc tgctctgctt cgttgagacg cagcaggccg
     1921 ttgtcgacga atacgcaagt caggttttta ccgatagcgc ggtgcaacag cattgcggtt
     1981 acggaggaat ccacaccacc agagaggccg aggatgactt tatcgtcgcc tacctgctcg
     2041 cggatgcgag ctacagcatc gtcgataatt ttcgctggcg tccacagggc ttcacactgg
     2101 cagatatcac gcacaaaacg ctccagcatg cgcataccct ggcgggtgtg agtcacttcc
     2161 gggtggaact gtacgccata gaagcgtttt tcttcgttag ccataatggc aaacgggcag
     2221 ctttcggtgc tggctacggt gacgaagtcg gacgggatag cggtaacttt atcgccgtgg
     2281 ctcatccaga catcgagcag cggtttaccg tctgcggtca gagcatcttc aataccgcga
     2341 accagtgcgc tgtcgtttac gacttcaacc tgcgcgtagc caaattcacg ttcgttagag
     2401 gcttcaacgt gaccgcccaa ctgcattgcc atggtctgca tgccatagca aacgccgaat
     2461 accggtacgc ctgcttcaaa aacatactgc ggcgcacgcg ggctgttttc ttcggtggtg
     2521 ctttccgggc cgccggaaag aataatgccg cttgggttga agtcacgaat ttgtgcttct
     2581 gtcacatccc acgcccacag ttcgcagtaa acacccagtt cacgcacgcg gcgcgcaacc
     2641 agttgagtgt actgggaacc gaagtccaga atgaggatgc gatgcttatg aatgttttcc
     2701 gtcattgacg cttattccga ggcaagtgaa acagataata taaatcgccc gacatgaagt
     2761 cgggcgaaga gaatcaggag cccagacggt agttcgggga ctctttagta atggtcacgt
     2821 cgtgaacgtg gctttcctga atgcccgcac cgctgatacg tacaaactcc gctttagtac
     2881 gcagttcgtc gatagtacca cagccggtca gccccataca ggagcgcagg ccgcccatct
     2941 gctggtgaat gatctctttc aggcgacctt tataggccac gcgaccttcg ataccttccg
     3001 gcaccagttt gtcggcagcg ttgtcgctct ggaagtaacg gtcagaagaa cctttggaca
     3061 tcgcgcccag ggaacccata ccacggtaag atttgtaaga acggccctgg tagagttcga
     3121 tttcacccgg agattcttca gtacctgcca gcatggaacc caccatcacc gcgcttgcgc
     3181 ctgcggcgat agctttggcg atatcgcctg agaaacggat accgccgtca gcgataaccg
     3241 gaatgccagt accttccagg gcttctactg catcagcaac ggcggtgatc tgcggaacac
     3301 cgacaccagt cacgatacgc gtcgtacaga tagaaccagg gccgataccc actttaacgg
     3361 cactgcaacc ggcttctgcc agcgcgcggg cacctgcagc tgttgccacg ttgccgccga
     3421 tgatttgcag gtcaggatat ttagcgcgag tttcacggat acgctgcaga acaccttcag
     3481 agtgaccgtg agaggagtcg atcagcagaa cgtcaacgcc tgcggcaacc agcgcatcaa
     3541 cacgctcttc gttaccagcg ccagcaccaa ccgctgcacc aacacgcaga cggccttgct
     3601 cgtctttaca ggcgttcggt ttacgttctg ctttctggaa atctttcacg gtgatcatgc
     3661 cgatcaggtg gaattcgtca tcaaccacca gcgctttttc aacgcgtttt tcgtgcattt
     3721 ttgccagcac cacttcacgg gcttcgcctt cacgcacggt taccagacgc tcttttggcg
     3781 tcatgtaaac gctaaccggt tgattcagat cggtcacaaa acgcacgtca cgaccggtga
     3841 tgatgccgac cagttcgttt tcttcggtaa cgaccgggta gcccgcaaag ccgttacgct
     3901 cggtcagttc tttcacttcg cgcagcgtcg tggttggcag aacagtctgc gggtcagtca
     3961 ccacaccaga ttcgtgtttt ttcacacggc gaacttcttc tgcctggcgt tcaatggaca
     4021 tgtttttgtg gataaagccg ataccgcctt cctgagccag agcaatagcc aggcgcgctt
     4081 ccgttacggt atccattgct gcggaaagca tagggatatt cagacgaata gttttcgtca
     4141 gctgggtgct gaggtcagca gtattcggca gaacggtaga atgtgcagga acgaggagaa
     4201 cgtcgtcaaa cgtcagagct tctttagcga tacgtagcat gggcaatatc tcgaccagag
     4261 tggttaataa atattgccgc ggcattatac agagcgtaac cgattgcatc tacccctttt
     4321 tgcaaaaaat gcttgctatc cccggagggc gggttactat cgactgaata acctgctgat
     4381 ttagaatttg atctcgctca catgttacct tctcaatccc ctgcaatttt taccgttagc
     4441 cgcctgaatc aaacggttcg tctgctgctt gagcatgaga tggggcaggt gtggatcagc
     4501 ggcgaaatct ctaatttcac acaaccggct tccggtcact ggtactttac gcttaaagac
     4561 gataacgccc aggtgcgctg cgcgatgttc cgcaacagca atcgccgggt caccttccgc
     4621 ccgcagcatg ggcaacaggt tttagttcgc gccaacatta cgctgtatga accgcgcggc
     4681 gattatcaaa ttattgtcga aagtatgcag cccgccggtg aaggtttact gcaacagaag
     4741 tacgaacagc ttaaagcgaa gttgcaggct gaaggtttgt tcgatcagca acttaaaaaa
     4801 ccactcccct cccctgcgca ctgcgttggg gtgattacct caaaaacagg tgccgcactg
     4861 cacgatattt tgcatgtgtt gaaacgccgc gatccgtctc tgccagtgat aatctatccc
     4921 actgccgttc agggcgatga cgcaccaggg caaatcgttc gcgccattga gctggcgaat
     4981 cttcgcaacg agtgcgacgt attaatcgtc gggcgtggcg gtggctcgct ggaagattta
     5041 tggagtttta atgatgagcg tgtggcgcgg gcgatttttg ccagtcgcat tccgatagtc
     5101 agcgccgtcg ggcatgaaac cgatgtgaca attgctgact ttgttgctga tctacgtgcg
     5161 ccaacgccgt cagccgccgc tgaagtggtg agccgaaatc agcaagaatt gctacgtcag
     5221 gtgcaatcga cccatcaacg gctggaaatg gcgatggatt attatctcgc caaccgcacg
     5281 cgtcgtttta cgcagattca tcatcgcttg cagcaacagc atccgcagct ccggctggca
     5341 cgtcagcaaa ctatgctcga acgtctgcaa aagcggatga gctttgcgct ggaaaatcag
     5401 cttaagcgtg ccgggcaaca gcagcagcga ttaacacaga ggctaaacca gcaaaatccg
     5461 cagccgcgta ttcaccgcgc acagacgcga attcaacaac tggaatatcg tttagcagaa
     5521 accctgcgcg cacagcttag cgccacgcgt gaacgtttcg gtaatgcagt aacacacctc
     5581 gaagccgtaa gcccactgtc aacgctggcg cgcggttata gcgtcaccag cgccgctgat
     5641 ggcgcggtgt taaaacaggt taagcaagtg aaagtgggtg agacactgac cacacgactg
     5701 ggagatggtg tggtgattag tgaagtcagc gccgtaacaa aaacccgcaa atcacgtaaa
     5761 aaaacgtcta atccgtagac cggataagag gcgccagcat cgcatccggc aataactccc
     5821 aggccacgcc agatccgcca tgctggttac tggaagcagg tcaccagacc cggtttattt
     5881 attagcgtga tactacaatc ctgtgctccg gtaccttcgt ccatcccaca ttgttgagag
     5941 gacaaataag gaaaactggg tgaagacggc catcccagtg aattaaaaac agtatgcaac
     6001 aactctaatt cattgtatgt cggtgcattt tctatgctgc cacactgcct tgcgatatcc
     6061 catttactgg catcaatgat atgtgcagaa gcccagtttt cgtagttaat aatcggtgag
     6121 gatacgggat tagccccgga cggcaattct gtcgttaaca acggtcgatg tagcgattta
     6181 ccattcacca ctaccgtgtc tgccatatgt ccccaaaagt gcgccgagga aacatttggg
     6241 ctggttggta ccgtaaaaat agcgtccagc gtgtctgtcg cggaagcgta attagccaga
     6301 gtaaccgtca gtggcgtttt ataaccaggg gtattatcct ggcggagcgt aaaacttgcc
     6361 gtcccatccg cgcctgtcat gccactaatg gtattaccgc tttcactcag cgtaacagcc
     6421 gaaccagata acggctgaac gatgagatcg tccatcgcca ccacatcacc atacaacgtt
     6481 gccccagcgt tacgcggcga tgcctcaccg cgtttcaaag taaattccac attcggcacc
     6541 gtattcccgg cgctatcttt caccgtcacg gtaacgggca tggcttcgcc ttttttcact
     6601 ttcgccgcct gagtggctgc atcaaacgct gttgaggtaa gtgtgacgct ggaaaccgtt
     6661 gaacgcgcgt tagccaggca aggctgaaat gattttgttg aacccgtata cagcgtgcca
     6721 tttctcaaat caaccgactg gtctttacca tcggcggatt tatcctctgc ccaccaagtg
     6781 taatacgcag gccagccagt ttcagtgaca aacgtgttat tcggataacg cgcagacaac
     6841 gaacttaaat cattgaatag tggctgcctt gctacttcac aagggctcac actcgcatct
     6901 gatttctgtg ttggggtaaa gtacggccag tcttcattat tgttattgat cttgccgacg
     6961 ccagaagtcg atgtcagttc actccacaat ttcgggcgtt caaataccgc accattcact
     7021 gccgtcaagg ttgctggcat atgcccccag tagttagcct tcgcagaatc cgggctggtc
     7081 actaccgtga atatcaggtc ctgattagcc gtaacctgcg gatttcgcgc caatgaagct
     7141 gtaaacggcg ttaccagacc gacggtgtta ttttgggtaa cgttgaacgt tatcgtgcca
     7201 tcactgccgg tgaagccgct ccatgccgac gtattctggt tgaaggagat acgcgccgat
     7261 gtcggggtga cttcatctaa aatgagatca tctacggtat tggaggatac agagatccct
     7321 gaacggcttt tcgcctgcac gcgcttgagc gtaacacctt cgccgccaac aggattacca
     7381 ttggcatctt tgatcgtcac cacgatggga agcatctcac cttttttcac agatgacgtg
     7441 ggggtaccac cattgctggc agttcggccc tcatcatagg cagtcgatga cagcgtaagg
     7501 cttgctggtg ccgaatgtgg ctgcgtcaga cagagcatta aagcgtatcc attaatagtc
     7561 gtcgtcgatt gtcccgtttt caaattaatg acgccgtaat accacgtcaa cgcgtcagtg
     7621 gtgctcattt cataagtcca ccaatttcca cttccggcag ttataggcaa ccccaaatct
     7681 gtcagtattg tcccgcctgg atgatcatta tatagcccct gtaactcatt gagacgcggc
     7741 tgatactcaa ccgggcaatc cggcgtaact ttattggtat aaaaaaggta tgaccacgac
     7801 tcattattat tacttaccga ggaccccaag gtcgcttctg ctgccagtag cggtcgttta
     7861 aaggtcaccc ccgccctact ggtaaacgtg tcgggcatat gaccccagta ttttgctttc
     7921 ggtgagttcg gactggttat cacagtgaaa ataacatcca tcgtggcact ttttgtggtg
     7981 tcgcgcatca atgttgcagt gagcggagtt gccagcccat aagtctcgtt ttgcgtcaca
     8041 tcaaaagtcg ctttaccatc acttcctgtc gtcagaagtg cctgtgcccc attattatag
     8101 agtgacatgt ttactccggc aggggtgacc ggcacgatag taagatcatc atacgtagaa
     8161 tctagcctgt tttttgcacg atccaacgca gtcccgcgta tgagattaaa gtttgcgcct
     8221 ggcaacagat ttcctgcgct atcccgcaca atcaccgtca tccggatcgc cgtacctttc
     8281 gccgccgttg cactagggcg accatcgttg ctggccgttt tctccgcatc cagtgccgtg
     8341 gaggtcatct ctatagaagc tgccactgca tgagggttta ccagacagga cacaaatgcc
     8401 acggtgtttg ataatgccct ggagcttgat ccattcgata aattaataga ctgatcgtac
     8461 cagacgttcg cgtaggcaat tttatcgtaa gcccaccagg tgttagtcag cggaaggcct
     8521 aaatcggtcc ctatcgcacc attaggatgt tcactgtata actcttgaaa ttcgcttaat
     8581 aacggttgat aaggttcgcc gcagtctgat ttgctgacat cattttttgc tgccgtattc
     8641 aaagaaagcc atgattcatt attagccatg gttgactgcc ccgctggatg ctcggcagta
     8701 agcaatggcc gcttaaaggc aattcccgac cgggtagtaa acgtttcagg catatgcccc
     8761 cagtaacttg ccagtggcgt atccgggctg gttatgacgg taaacatgac cggtaatttg
     8821 ttggattcta tcgtcggctg atacaactgg gcgtacatat tggttaccag gcccatagaa
     8881 tcatcttgtt ttaccgtaaa ggaggtagtc ccatccgcgc cagtcacccc ataaagcagg
     8941 cttgaagaac tattcatcct cgcagaagcg cctgccgcgt taattaccgt aatcgactta
     9001 tttatcatct cggcattttg acggttcttc gagccttcac gtctgagaat aaaggggaca
     9061 ttgggcacgc tatttccctg cgcattttta gtcaccacgc gaatcaccgc gtcttcgcct
     9121 tttttcactt tcaacgcact caaggtgtcg tcaactttga caaattgcgc cgtatcattc
     9181 gcctcaacga tgatctgact ggcaacggcg acaggcgtgg tccggcagct cagtaatgca
     9241 ccaccagatg agggcatctg cgtttcatct cctgtcttca gcgaaacgac atcatacgta
     9301 aaggtttgag tgaacgtatc tgcaacggta ttcgagatat agaacctcga cactggccag
     9361 ccaatagcat catgtaatgc gctactcccc tggtgcgcat aaatggccct aagttcattg
     9421 tcagttggaa catactccgc gccacaacca tttatcgagg tatcgttagc ctggcccgac
     9481 gggaacgtag accaggcctc cccctcttcc accagggtac cgatactggt ggtagcacta
     9541 ttctcgtccc tcagtaaagg ccttcgataa ataagaccgt catcgcctgt cgccgtttca
     9601 gccatatgcc cccagaattt ggcttttggc gaatccgggc tggtaagcac cgtaaagaca
     9661 acgggaagcg tcgatttagt gctgctggag gaatccaacg ctgccgtgag ttctgttttt
     9721 agcccggtag tctggtcttg cttcaatgtg aaagtggtga tgccatcagc ccccgtcacg
     9781 ccataaataa ccagggtcgt tgaaagaaaa tcatcttgag tatttcccca ggcatccgtt
     9841 accgccagcg aagcaatcga cgtcgtagtt gacacattgg ccctgttcac gctggtgttt
     9901 cgtttgaggg taaatgcggt atttcccaca gggttaccct gcgcatcttt agtggtcact
     9961 cgtaatacgg cttcttcgcc ttttttcgct ttgacaacat tattcgtact gtcaaatttt
    10021 gtggggtctg cagcttcaag aacaatttgg cttgccttaa caatcggcgt cgtctggcag
    10081 gtaagcagtt cttttgtggc agatggaatt atcgaactgg tgcctgaact gagattgacg
    10141 gccttgaagt cacgctgttc caggctacta ctacctgtcg tgctggaaag gtaacttgag
    10201 gcgacgggcc agccctgaac cgttttcatc gcattgccgc ggttggcttc atacaatgac
    10261 tccagcccgg cctgggaggg aacatattca ctaccacaac cattagagga tgtgcttata
    10321 gcctgggttt gggtaaattg cgcccagtct tcattctctt ctgcaatcgc tgttcgaccc
    10381 gacgtactgg ataactcttt caacaataat gggcgtttaa agaccgttcc atctgcagcc
    10441 gtcactgttt caggcatatg cccccacatt ttcgccttat cgctgtccgg gctggtgaca
    10501 acggtaaaaa tggtatcgag ggtcgctttt ttcgttgtgt ctgaatagag cgcggcggtc
    10561 agcgacgttt ttgtgccatg ggtatccggg cgggtaatag tgagaatttt agtgccatcg
    10621 ctgccggtca tcgcgctgta tgctgcggcg gtatcattga gcgaagtttc atccgccagg
    10681 ccactattga tcacaaccgg cgcaaccagc gcatcaccgc caccggcaat gtgtttttcg
    10741 tttgatcggg tataaccatc gccacgcttc agcgtaaaag gcatatcacc caatggattg
    10801 ccctgagcat cttttaccgt caccttcacc tgtagcgttt cgcctttttt cagcttcgcc
    10861 gcatttgctg ccgcgttcca ctgcgccggg tccaccactt ccagcgtaat gctgctggca
    10921 ggattgttag ccgtcgtcag gcaactgaca taaaccgccg tgctaccgcc aacggtccgc
    10981 gcgccatcgt tgagggctat ggtgtagtaa tgcggcacct gatccgcggg cgaactgctc
    11041 cagtagggct gacgctgcgt tggccaaccg tgtaccgttt gaacggcgtt accgctgtta
    11101 gcatcataga gcgcgttaag ctgggaacgg cgtgggagca tattggcacc acagccacct
    11161 ttactggtgc tggaagtgtt ggcctggcta acgcgcgacc acacttcgtt attttccgtt
    11221 aatgtgccgt ccgggctgga cacttccgca gccagccggg gtcgggcaaa ggttaatgca
    11281 tcgacggtaa tcgtctcatc catatgaccc cacatctgcg cgccaaccgc atccggactg
    11341 gtgagcgtag tgaaaatcac attgtaatta acagtattgg gaatatagga gtttacaggc
    11401 gcaatattca gcggcgtcgc taagcccaca ccttgcggtt gcttaatttc cacagtagca
    11461 aaaccctgcg catccgtagt accggcatag actaaagacg gttgcgatgt tccgtagagt
    11521 gtgccattca tctcgattgc gccgcgggtt ggatcatcaa aacctgtagt ctgcccctga
    11581 cgatttttcc ccatatcttt agtgatggtg aacgcggtat acggcacggg cgcattattc
    11641 aaggcattaa aggtgcgtac cgtcatggta atcgtttcac ccactttagc ctgcgctctg
    11701 ctgcttgtgg aaacatcatg atcggtggtc gcagcaatct gggcgaccat ctcattgccc
    11761 gaacaagaaa ggtagttttg cttaaagccg gaataggtgt cgatgttgcc cgtggccagg
    11821 ttcaccgacg cgtgcgacgt ctcttccacc gcactaagat acgctttttg caacgttggc
    11881 cagccatatt cggtgccgat gacattggct ggatgtgcgg caaacaaact gtgcaatgag
    11941 ctttggctcg gaatatgacc taaaccacac tcagcctgca tactgctatt ctgatcaaac
    12001 agcgcccagt cttcgttatt ttcccgcact gctcccagtt cgttgtcggt ttcatccgcc
    12061 agccgtgggc gtttaaagat attattcgct tcaataatgc ctaacatatg tccccacatc
    12121 cgcgctttgt cggaatcagg gctggtgatc accgtaaaaa taacgtcttt ctcatccgag
    12181 gcggtataat cactgcgcat tttcgcggtg atgtgtgttt tcacccccac gccgcctggc
    12241 tgggtcagcg tcagagaggc ctctccgtta gcatcagtga tcccttcata ggtatgcgcg
    12301 tcaactttgc gaacgttact gccaccatca atttgtaccg ggtgcgtctc ccacgcagcg
    12361 tcagtttgat tcttacggtt aacaccgtca tccagatgca gcgaaaagta gtagtaagcc
    12421 agcggctgat cgttgttctt gctatcggta atcgtcaggc gtaaagaagc ctcttccccc
    12481 acctttactt tcatcgcttt tatcgtcgaa tcataactgc ccggcgtcag taccagcttc
    12541 ggttcaacat cgggcgcttc tttatcgaca cagctcacaa taaacgtcgt atcgtccggt
    12601 ttttgcgctt cgctgcggtt cgaaacatcc gccgcatagt gctgcccgct catccccgcc
    12661 agtgatgacc agtagtagtt accctgcatc ggccaaccag ccacatcctg cactgacgtt
    12721 ggcaccaccg tcgccagcgc accgaactga cgcattccgg gcaaaatgtc gcaatgttta
    12781 tccgcaccac tccaggtaaa ggtggaccag gtttcattgt gatcgttaac cgtaccgtct
    12841 tcattatcga cttccgccgc cagttttggt cgactaaaag tgtaatcacg ggccttcagc
    12901 gtatcgggca tatgacccca cattgtggcc tgcgggacat ccgggctggt gaggacggta
    12961 aagatcactg ccgtttcact ggtctgagca atacccgcga ggctcgcgac caacggcgtt
    13021 tttacaccag ggccgttggc ctgggtgacc gtaaccgtgg caacaccatt cgcatcggta
    13081 gtaccacgat attccgtcgc ggtggtggtt aattcggtcg tgcccagttt taccggcgca
    13141 gtattgttca ctacaccctg gcggttttca gcatctttgc gtttgataac aaaaggaata
    13201 ttccccacaa cattaccgac gcaatctttg gtggtgaccg ttaacgtgat actgccgcca
    13261 accgtcgttt tcgccgcacc ggaggattcg tcatagcccg cggcgctgac ggaaataagg
    13321 tcaccctgcg cgggcgcaat cttcagcgtc actttctgat cggcaagggt cagaacactt
    13381 tcatagggat agcccgtcga ggtaagtaga tataacagcg gcatggtctg ctgggggtct
    13441 ggctgcgccc aggcgatcag cgaacgcccg gcaaggccct gtgcgcaaaa tgagcgatct
    13501 gcgactggct gtgggtccag taaggtgtcc ggcgtcgcgg catccgccca aactaaagat
    13561 actgtcggtg gcgtttggtt ttcccaacgt ggtgtggcaa tgatatcgcc ttctctgtct
    13621 tgcgggttgg tgacgagcaa tttagagacg ctaacatccg cgctgaattc actcggttta
    13681 gcggtaaacg ctacctcatg cgtctttgca ggatccagaa agacactgcc ctggtagacg
    13741 ggtatgctgg cactgtcggc ggtgggcacg gtaccgttaa attcgccgac cgtatctgaa
    13801 gagtcttgcc atgcagattc tgcccatgac cacccaggca gcattgcgct tatcagcaat
    13861 gcgcataaca caaggatttt attcagggtc agggacatga cagaaactcc accggttgtt
    13921 aaacggacgc ttgtcgcatc aacatatttt gcttaatccg ggattaagca aaatatgcgg
    13981 aaacagcatt cccttgcggg aatgctgctg agtacgcgtt atttgtgttt gtaaataatg
    14041 tgaagtttgt aaccctgtac cccgtcgccg tttgtcggaa tggtaagagc ctctttacca
    14101 tcgcgcgcag gaccgttaag gttggttctg gcttcgtcgt tggtcgcagg gatggccaga
    14161 ttgttattat cgaagcttga gttaacgata ccacctgaag tgcccagctg ctggctgctg
    14221 ccagaaagta cccaacggaa gtcataattg gcggtaacgt cagcatcggt acccgcatca
    14281 aacttgccgt ttttgttctc atccgagtac agtttggcca cgtaggtttt gccaagttcg
    14341 attggcgtgc tgccgttgat aaggttgata ctggtaccat caacaaagat ggcgactttc
    14401 aggttctggt taaccaccgg ccctggcgca acgttatcgc tgtcgcttcc gccgccgctg
    14461 gcagtggaaa tgtcataaag atgcagcgca gtcccgacgt ttggcgtacc ggtctgcgtg
    14521 gtcggcgtaa tttcaatacc gatataacga ccacggtcgt catcggtaag agtgtaactg
    14581 gtcaccttcg ttgccagctc tttcggatcg cccccctgag cgtccttgta gctcatccag
    14641 cggatagtat cgatagtctt tgcgttatcg cccaatcccc caagatccac gtcgccttcg
    14701 gtatcaccaa tttcccaact tacggttaat ttatcacccg agtaaagacg cttgtccgtt
    14761 tcactgcctg tcgggcgagc gccttttaga tcagaagtca acgttacatg gtcggcatct
    14821 gtcgtagcgc ttcccccttc cgaatagagc cacggaatgg ttccctggaa aatattggtt
    14881 tcgccgctgt cgacccaggc gctttcttcc gccagcgcca caggtgccac acaataacct
    14941 gccagtgcca gcgccaatgc gatccgggtc agaccgcgtc ttttatcctg cttcattgat
    15001 gctctcctta atgaaccttc agcgactggc cgactggcct tacgccaatc ttattttttg
    15061 gtatacagca cgcgtaaacc gtatccctgc aagccatctc ccgcttgtgc gccataggtt
    15121 tgcgctgctt cgcggttagt ggccggaatc acaatgtcct cattggaagt gttcgcctgt
    15181 gcacccactt caccattgat gctcttgccg gtaaattccc acttgtagtc gtactgcgcc
    15241 atctcttcgt cagtcagttt ttcgacctgc tggaagacac cgtctttatt gctgtcacgc
    15301 cacaaaacaa atcgataagt gttgttgagc ggcagcggtt ctttatcttt acctatcagg
    15361 ttgctgggtt tagccgcccc cacacgatag ataaatgcat ttagcccacc aatttctgcc
    15421 ccgaggaaag tgacatccac atagttactg tcgtcgtagg gcgcagcttt tgctttccag
    15481 cgataggtgc cgggtaaggt actggagagg gtgacagtac ccttttcttt atcttgttta
    15541 gcgataaaaa cgcaggggtt gtcggtcgag caaggcttgt tggttaacgt cactcgtttg
    15601 tcctgcgcgt cgtaaagccg cggcgtgagg ttatcttccg gcagcgcatc gccgttgcgg
    15661 tcggtgttat cgccaaagct gtcggttaca tcgagatcca tcaccacacc atcaacgcca
    15721 tctgcctggg cgctggcttc gtggctgtgg ttgttctctt ccggcacgtt cgcgatgtta
    15781 ttaacaatca actcaatttt acggtcctgc tgaacggtga tttccaccac accagagttc
    15841 tgtcggttgc ccttttcatc ttccagcgcc agacgtactc gccaggtatt gagtttttct
    15901 tgttcggctt tgtcagagct gtactgccag gcgggcagca ccaggttcca gtggttattg
    15961 ttaccctctg tcagggtatt ctctttgata accggcataa ccagcgtgcg ggcagcattg
    16021 tttttcgcct gccatgacgc agcgacaatt ttgtagtggt ggttgataag cgcgttaatg
    16081 gtccacttac atttttccag cccgaccgcc gcttcaggcg tgtctttaat gacacactcg
    16141 gggtgttcgt tggtggcatc cgcttttaac cacagggtga catcaagcgg atctttttct
    16201 ttgtattcca gaacaataaa gttattacgc tcaacaaaat catgacgact gcccatcaac
    16261 gagtgagccg ctttcacttt atcgctatcc aattgatctt ttagcggaac gccgaactga
    16321 taattaagag tggcattaat gctgacatcg ctgttatctc cggtccccgc tttatagtcc
    16381 gccccaacgg ttagtaatgg caccggttga taattcagtc caagtgttac cgcgtaggga
    16441 tctttctcca aattatccgt accaaaaagg gcaacttcat cgccgtaata ttgctcgaag
    16501 acaactttgc cccccagttg cggataagaa ggtaaccagg cttcggcgcg aatatcccag
    16561 ccgcgcgcag gacgctcctc ataaaaatcg aaatcttcgg agtctttcca gtcagaaagc
    16621 ggatgataat agttgccaga gaattttaaa taatcagtcc acgcttcgcc gcctaaacct
    16681 aaacggcgat gtcctcgggt aaaatcataa tcataaaata tatttccgcc caataaccat
    16741 tcatcaaaat tatatcttac cccaagacca atattcccga ttgtacgatc ttcttttcgc
    16801 tgcgcagaaa attgactgaa ataaacagtg gtttgattat cgtaccaggg aacaaaataa
    16861 tcgatggaac tgccatccag atcgccgccc tggccaatgg agaggttcga acggacctta
    16921 ccgtagggag agagagtgtc ttcaatataa gattgtgcgg ttgaggtgat ttgactttgc
    16981 agatagcttc tggcctgaga ttccagcgcc tcaggggtca agttttcaaa cccttgcgtg
    17041 gcggagttga tgatgtaatc ggctccgcgc tctttcagcg atttaccctt gttggtttgt
    17101 tcatcctgct gtgctgcctg gctacctaaa tcaggtaacg attcgttttg cttaacattt
    17161 atctctggcg ccgcgaaagc agcaccagag ataaatgtta gaataataca gcgtttccat
    17221 cgcagcatgc actttccctg tgacaatgat acagtcaaaa ataagcttta atatt
//
LOCUS       DAZKGF010000106        16868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZKGF010000106 DAZKGF010000000
VERSION     DAZKGF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.106"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1326
                     /gene="xanP"
                     /locus_tag="ACHOQV_003267"
     CDS             <1..1326
                     /gene="xanP"
                     /locus_tag="ACHOQV_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFP9631593.1"
                     /translation="IYRLEDRPPLPQTLFAACQHLLAMFVAVITPALLICQALGLPAQ
                     DTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQGTSFNFVAPLIMGGTALKTGGAD
                     VPTMMAALFGTLMLASCTEMVISRVLHLARRIITPLVSGVVVMIIGLSLIQVGLTSIG
                     GGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNPYLRVASLVIAMAAGYALAWFMG
                     MLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLMLVFMITSLETIGDITATSDVSEQ
                     PVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCFGQNNGVIQLTGVASRYVGFVVA
                     LMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIAASGVRIVSREPLNRRAILIIAL
                     SLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGITAIVLNLIFPPEKQ"
     gene            1447..3156
                     /gene="yicH"
                     /locus_tag="ACHOQV_003268"
     CDS             1447..3156
                     /gene="yicH"
                     /locus_tag="ACHOQV_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP9631594.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGSWLRDGKTLLLDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVTGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(3200..3880)
                     /locus_tag="ACHOQV_003269"
     CDS             complement(3200..3880)
                     /locus_tag="ACHOQV_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFP9631595.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(3861..4793)
                     /locus_tag="ACHOQV_003270"
     CDS             complement(3861..4793)
                     /locus_tag="ACHOQV_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9631596.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            complement(4841..5701)
                     /locus_tag="ACHOQV_003271"
     CDS             complement(4841..5701)
                     /locus_tag="ACHOQV_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9631597.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPT
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            complement(5782..6633)
                     /locus_tag="ACHOQV_003272"
     CDS             complement(5782..6633)
                     /locus_tag="ACHOQV_003272"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9631598.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASVQLCVEGELGHLADAPRYDQAANADLMTQPTDVET
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEISRCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            complement(6645..7736)
                     /locus_tag="ACHOQV_003273"
     CDS             complement(6645..7736)
                     /locus_tag="ACHOQV_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9631599.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            complement(7761..8075)
                     /locus_tag="ACHOQV_003274"
     CDS             complement(7761..8075)
                     /locus_tag="ACHOQV_003274"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP9631600.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            complement(8093..8563)
                     /locus_tag="ACHOQV_003275"
     CDS             complement(8093..8563)
                     /locus_tag="ACHOQV_003275"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFP9631601.1"
                     /translation="MDITKILNTNRVILDMQATNKEEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            complement(8590..10143)
                     /locus_tag="ACHOQV_003276"
     CDS             complement(8590..10143)
                     /locus_tag="ACHOQV_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9631602.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENITDLLHLDHLLVAECEEIDGVMNNSRRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            complement(10439..12757)
                     /gene="yicI"
                     /locus_tag="ACHOQV_003277"
     CDS             complement(10439..12757)
                     /gene="yicI"
                     /locus_tag="ACHOQV_003277"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFP9631603.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKHPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAVCEMPAADGSVIFTLKATRTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLMVTPQGNALTITL"
     gene            complement(12767..14149)
                     /gene="yicJ"
                     /locus_tag="ACHOQV_003278"
     CDS             complement(12767..14149)
                     /gene="yicJ"
                     /locus_tag="ACHOQV_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFP9631604.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            14442..14536
                     /locus_tag="ACHOQV_003279"
     tRNA            14442..14536
                     /locus_tag="ACHOQV_003279"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14476..14478,aa:Sec,seq:tca)
     gene            14836..16041
                     /locus_tag="ACHOQV_003280"
     CDS             14836..16041
                     /locus_tag="ACHOQV_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9631605.1"
                     /translation="MALTDIKVKTAKPKEKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGVAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFKKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLACALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFKTKLWEIPAEVMKMKRPHIVP
                     LSEQVIALFKQLEPITKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIETQLAHVDKNAIRGTYNHALYLDGRREMMQWYADYIDSLSSH
                     ESQCQGPLY"
     gene            16449..16715
                     /locus_tag="ACHOQV_003281"
                     /pseudo
     CDS             16449..16715
                     /locus_tag="ACHOQV_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4374 a         4374 c         3910 g         4210 t
ORIGIN      
        1 atttaccgtc ttgaagatcg tccgccgctt cctcaaactc tgtttgccgc ctgtcagcat
       61 ctgctggcga tgttcgttgc ggtgatcacg ccagcgctat taatctgcca ggcgctgggt
      121 ttaccggcac aagacacgca acacattatt agtatgtcgc tgtttgcctc cggtgtggca
      181 tcgattattc aaattaaggc ctggggtccg gttggctccg ggctgttgtc tattcagggc
      241 accagcttca actttgttgc cccactgatt atgggcggta ccgcgctgaa aaccggtggt
      301 gctgatgttc ctaccatgat ggcggctttg ttcggcacgt tgatgctggc aagttgcact
      361 gagatggtga tctcccgcgt tctgcatctg gcgcgccgca ttattacgcc gctagtttct
      421 ggcgttgtgg tgatgattat cggcctgtcg ctaattcagg ttgggttaac gtccattggc
      481 ggcggttacg cagccatgag cgataacacc ttcggcgcac cgaaaaatct gctgctggca
      541 ggcgtggttt tagccttaat tatcctgctt aaccgtcaac gtaaccctta cttacgcgtg
      601 gcctcactgg taattgcgat ggcggccgga tatgcgctgg cgtggtttat gggcatgttg
      661 ccagaaagca acgaaccgat gacacaagaa ctgattatgg tgccaacgcc gctctattac
      721 ggtcttggca ttgaatggag tctgctgctg ccgctgatgc tggtctttat gatcacttcg
      781 ctggaaacca ttggcgatat cacggcgacc tctgacgttt ccgaacagcc agtgtccggt
      841 ccgctgtaca tgaaacgcct gaaaggcggt gtgctggcaa acggcctgaa ctcgtttgtt
      901 tcggcggtgt ttaacacctt cccgaactcc tgcttcgggc agaacaacgg agtgatccag
      961 ttaaccggtg ttgccagccg ctatgtcggt tttgtcgtcg cgctgatgtt gatcgtgctg
     1021 ggtctgttcc cggcagtgag cggttttgta caacacattc ctgaaccggt tctgggcggc
     1081 gcaacgcttg taatgtttgg caccatcgcc gcctccggtg tgcgtatcgt ttctcgtgag
     1141 ccgctgaacc gtcgggcgat tctgattatc gcgctgtcgc tggcggtcgg tctgggcgtc
     1201 tctcagcagc cgctgatttt gcagtttgcc cctgaatggc tgaaaaacct gctctcctcc
     1261 gggattgccg cgggcggtat tactgccatc gtgctgaatc tgattttccc accagaaaaa
     1321 cagtaattca ctggcggcgg taagataatt accgccgcag tctcacgtct ttcaccattc
     1381 ccgccttgag gattgcgcgt aaatcgtgca taactccctt atgtgctttt cacgggatgg
     1441 aagaccatga aatttattgg gaagctgctt ctctacattc tcatcgctct gttagtggtg
     1501 atcgctggcc tctattttct tctgcaaacc cgctggggag cagaacatat cagcgcatgg
     1561 gtttccgaga atagcgacta tcatctggcc ttcggggcga tggatcaccg tttttccgcg
     1621 ccatctcata tcgtgctgga gaacgtcacg tttggtcgtg atggtcagcc cgcgaccctg
     1681 gtggccaaaa gtgtcgacat tgcgctaagc agtcgccaac tgaccgaacc acgccatgtc
     1741 gataccatct tgctggaaaa cgggacgctg aatctcaccg accagaccgc gccgctaccg
     1801 ttcaaagccg atcgtctgca actgcgtgat atggcgttta atagcccgaa tagcgaatgg
     1861 aaactaagcg cgcagcgggt aaatggtggc gtggttccgt ggtcaccaga agccggtaaa
     1921 gtgctgggta cgaaggcgca gattcagttt agtgccggat cgctttcgct caatgatgtt
     1981 cctgccacca atgtactgat tgaaggcagt attgataacg atcgcgttac gctgactaac
     2041 ttgggtgccg acatcgcccg cgggacatta accggaaacg cgcagcgtaa cgccgacggc
     2101 agctggcaag tggaaaatct gcgcatggcg gatatccgtc tacaaagcga aaaatcgcta
     2161 accgacttct ttgcgccatt acgctctgtc ccgtcgttgc agattggtcg cctggaagtc
     2221 attgacgctc gtttgcaagg tccggactgg gcggtgaccg acctcgatct cagcttgcgc
     2281 aacatgacct tcagtaaaga tgactggcag acacaggaag gcaaactgtc gatgaacgct
     2341 agcgagttca tttatggttc gctgcattta tttgacccga ttataaacgc ggaattttcc
     2401 ccgcagggcg tagcgctgcg ccagttcacc agccgctggg aagggggtat ggtcagaacg
     2461 tcagggagct ggttgcgtga cgggaaaacg ttactccttg atgatgcggc aattgccggg
     2521 ctggaatata ccttgccaaa aaactggcaa cagttgtgga tggaaacgac acccggttgg
     2581 ttaaatagcc tgcaactgaa gagatttagc gccagccgca atctgatcat tgatatcgac
     2641 cctgacttcc cgtggcagct caccgcgctc gatggttacg gtgccaacct gacgctggtt
     2701 accgatcata aatggggcgt ctggagtggt tcggcgaatc tgaatgccgc cgccgcgaca
     2761 ttcaatcgtg ttgatgttcg tcgcccatcg ctggcgctga ccgccaacag cagcacggtg
     2821 aatatcagcg aactgagtgc atttactgaa aaaggcattc tggaagccac cgccagtgtt
     2881 tcacaaacgc cacaacgtca gacccatatc agcctgaatg gacgcggtgt gccggtgaat
     2941 attttgcaac agtggggatg gcctgaatta ccgttgactg gcgacggcaa tattcagctt
     3001 actgccagtg gcgatattca ggccaatgtc ccgctgaaac ctacggttac cgggcaattg
     3061 catgccgtga atgccgcaaa gcagcaagtg actcaaacca tgaatgcggg cgtagtttcc
     3121 agtggtgaag ttacgtcgac ggagccggtg cagtaaaaat aaggacgcta gcggatatca
     3181 gcgctagcgt tccccgttat tagcgcaaaa ttccatactc actgcacaat aaccatttca
     3241 ccggttcatc ttcttctatc gccggaaaac gtcctcccgg ctcaagaaaa taattatcgt
     3301 gttggtcatc atttaccatc gagatttccc agccgagaac aaacgctttt tccgcccaga
     3361 agcgatgata cagacgcggc ggcaggcaaa tgccctgccc cggctttagc accagcgttt
     3421 caccaggttt catggtacgc cgctgaccat caacactcac ggtaatgact cgttgatcat
     3481 cacagagatt ggcttcgttg gcccaggcca actgcatgca taagtctcca ccgccccgat
     3541 tgaggatatc ttccattttg tgggtgtgaa aatgccaggg agtctgctga tcttgttgga
     3601 tctgcaacat tttctcagca tacggttttg gatatgccgc cgaccccagc gcgccgttgc
     3661 gcatcgttag cagtgttagt cccgtttggt tgaaatcacc acgaccaaaa tcggtgatat
     3721 cccaaccgag ctgtaagtcg accacttctt gccagttatc ccgttcccgc tgcagccagt
     3781 catccaccgt aaaataggcg aactctggca aacaaacgcg gaaggtctgc gcaatggcat
     3841 gtgctttatc aataacataa ttaatttgtg aacgtttcat tggcttctcc gttgcgcata
     3901 gcaagccagg cagcgccacg cattccgcta ctgtcgccgt gctgtgcttt tacaatggga
     3961 gtggtgaatt gatcggtgaa aaccagtgga gcgacttctg cgttcagatc ggtgataagc
     4021 agttccacat tcgaaagccc gccgccgatc acaatcacgc cagggtcaac aacattaacg
     4081 atggttgcca gcgtgcgggc aagctgttgg cgaaaacgtg ccacctgctg catggcacaa
     4141 gcatcaccac gttgtgccag ggtgacaatc gccgcaggag tgagcaagtt accagtcatc
     4201 tgttgatagc gttcactaaa accgctgccg gaaacaaacg actccacgca gttgtgtttg
     4261 ccgcaatagc aactgactga tggaccatct tcctgctccg tatagcctgg caacgtgata
     4321 tgaccgcatt cggcagcatt tccccaggcc cctataaacg gtcgttgctt gatggcaatg
     4381 ccaccgccgc agcccgtacc aagcgtaata ccaaatacca cgtcataatc ttgcccggca
     4441 ccgtcgcaag cttctgatag cgcgaaacag ttaccatcat tggctatcac caccggttgc
     4501 ccaagcaatt gctgcaaatc agcctgtaac gcatgaccgt taatcacctg aatattcgcg
     4561 tttttaatca ggccagtgag tggcgagata ctgccgggta aggcgatccc tgtcagcatc
     4621 ggtcgttgaa cgtcccgcct aatctgctcg ataagcgcca caacgcatga gacaaattgt
     4681 tgatatgtcg acttttgcgt cgggcagcgg taacggcgaa tctcccagcc atgcgcatcc
     4741 atgacgacgg cggcgatttt ggtcccacca atatccaacc ccagatagtg catatctcgt
     4801 tccttgttgc gtaaaaggcg ggtcaccccg cccttctggc ttagtacagt gatgctttac
     4861 cgagcgaacc gaacagtttc attttgtggc gaatgacttc ccgcgctgcg ttaatcgcat
     4921 ccggatagat gacgttcggg tcccaccagc tctctttagc gagaatttca cgcactttct
     4981 ggaaataggc gtatttcata tcgctggaga tattgatttt gccgacgccg agcgtaactg
     5041 attcagcaat ctccgcgtcc gggtttgccg aaccaccgtg tagcaccagc ggaatatcaa
     5101 tacgtccggc aatgtcgcgc aaaatgtcca tttgcagctt cggctgcatc ccttttggat
     5161 agatgccatg cgctgtacca atcgccacag ccagtgtgtc ggcacctgta cgagcaataa
     5221 aatcggcggc ctgcgccgga tctgtgtagg tcacttgtga aacaccacct tctaccgacg
     5281 ttcctgtttg tccgatggtt cccagttcac cctcaaccga tactccaacc gcgtgagcga
     5341 ggcgaaccac ttctgcggtc agcgccacgt tttcttcata tggcagcagc gaaccgtcga
     5401 tcattactga ggtaaatccg cagcgaatcg cacgcaaaac atgctctaca gaggcaccat
     5461 gatcgaggtg cagtacaaac gggaccggac tacgctgggt aatttcgcga acgtgagcga
     5521 aaaaggcatc gcccacaaaa tcatgttcgc tgggatggat ggcaataatc gtcggagtat
     5581 ttgtcgcttc tgcttcatcc acgactgcgc gaaggaaaca gttatccgcc acattaaatg
     5641 cgccaatggc aaagccgtgc tggcgggtag gttgcagtaa ttctttcatc gaaactaaca
     5701 tgttaaatct ccagtttcag cgtgtagtcc gccctgccgg tgcgttattg cactggcata
     5761 aaggccatga cagaaaatga atcaggcttt gccgatagcg ccgctcgcct gtaaatacgg
     5821 tgtaatcgct tcttcgaagg ccagttccat agctttcagc gcgtccacca gttcaatgga
     5881 tatatcgtga cagagggtat gcaggagcgc ggatttcccc cgttcaaaaa tggccgcccc
     5941 cacgttgatt tttgctaccc ccagcgtcgt acagagctgg agctgatcga acggcgtacc
     6001 gctaccgcca tgtaatgcca gaggcacgct cgagcagcgc gaaatctccg ccagacgctg
     6061 aaaatcaata gcaggtacaa caccaggcgc gtacatgccg tgcgccgtgc ctaccgagac
     6121 ggccagtaga tcaatgcctg tctgcgcgat aaacgtttcc acatcagtgg gttgcgtcat
     6181 caggtctgca ttggcggcct ggtcgtaacg cggcgcatct gccagatggc ccagttcgcc
     6241 ttccacgcag agctgcacgc tggcggcgag ttccaccacc gccctggtct ggcgcacgtt
     6301 ttcctccagg ggaaaagcag aagcatcaat cattaatccg ctgaaaccag cacgaaacgc
     6361 ctgacgaata cagtccggat ttttgccatg atcaagcgcc agagcgaccg gtactgaggc
     6421 agattccgcg agcgttttaa ccactgccgc cgcgacgggt gcaggaaaat gcgccagatg
     6481 cccctgatag agattgagaa tgatcggagc gcgattcttc tccgccacgc ggatagctgc
     6541 gtgagcactt tccagattca tacagttgat cgccagcaaa gcgtaatgtt catgccaggc
     6601 tttcatcacc atacttttca tatcggcaaa catagggcct ccggttattc gagggtgaaa
     6661 gtgatctctt catctttcag cttttgatcg ctcagttcgt caaactcttc gtcgcgttcc
     6721 gttaccggtt tcttccagag tgaaagcatc acgccacaaa tcaccgtgcc tatacccagc
     6781 gccaggcaaa acagcagagg atgggtgaac agaggcacaa caaacatgcc gccatgcgga
     6841 accggactct ccacgcccca caccatgatt aagccgcccg cgaccgccga tgccaccaca
     6901 caagatccca ctactcgcag caggtcacgc gcggcgatcg gaatgactcc ttcagtgatc
     6961 atgcagatcc ccatcggaaa cgcggctttc agcgcctcgc gctccgcacg ggtgtattta
     7021 tggcgtgtca gtaagaacga aagtgtgatg ccaaacggag ggatgattga gccgacaaat
     7081 ttcacggctt ctggtccgta gacgccactg accagcaagc catcggaaaa cagcgacatg
     7141 gttttgttga ccggaccacc gaaatcgaag gttgccatcg cgccaagtat ggcccccatc
     7201 aggaagcgcg atccgccctg catcgactcg agcaaatgaa tcagtgcttc ttgtaaccag
     7261 gcgatgggtt taccaatcag cgtcatcatt aacagcccac cgataatggt gcttaatacc
     7321 ggcaacacca tgattggcat gagtccttgc atcgactgcg gcaggcggat atagcgttta
     7381 agtagcaaaa tggtgtaacc caccaggaaa ccacccaaca taccgccaat aaatccggta
     7441 tggatttgcc cacagagaaa gccgacaatc aggcccgggg caaatccggg acgatcggca
     7501 atagaatagg caattgccgc gctaatcagc ggcacaatca gccccattcc ccagccgcca
     7561 atctgattaa gcatccacgg gattgttcct gttttttcac caacatctgt accaccaaga
     7621 acttgcccca acgcaataca tatcccggcg gccacaataa ggggtatcat ccacgagatg
     7681 ccggttaata aatgtttttt aatttcctgt gaaaccggaa ttttattttc tttcatatta
     7741 agatccctcc agtataagga tcatttgttt cccagtgatt tttcgacctt ttcaagaaat
     7801 tgaacgggcg atttaatgac gatctctgtc tttactcgca caatacgttt attggtgaag
     7861 cgttcttctc cttttatttt cacatctaca gcaagaatca cgacgtcagc agcagagata
     7921 tctgccgcct gtaattcatt ttctgtacca atggttccct gggtttcaac tttaatggta
     7981 tgacccagag cttttgctcc tttgattaac ttttcacgag caatataggt atgagcaata
     8041 cccgccgtac aggcggcaac acagacaata ttcatgacat actccctgtt atttagcaga
     8101 catcggattg tgcatactga ctaaagagat caatgatttt ttgcggactc gtctcaacta
     8161 acaattgttc tatgatgtca tcatccgcca gcgcgcccgc gacctgggaa agtaaacgga
     8221 tatgggtggt attttgatct tcaagacgta ccgcaaataa aataatacag cgcacgctac
     8281 tgccatccag cgtttcccag ggaatatcct gccgggtacg gccaattgcc agcgtagtat
     8341 taatcacagc tgaagattta ccatgcggga tcgcaatatg attttcaaaa ccagtcgagc
     8401 cttccgcttc gcgctgccag acatctttaa taaaggtttc ccgacaatcg atcgcaccat
     8461 cttttttgag aagattagtt aattcttcaa tcgcctcctc tttattcgtc gcttgcatat
     8521 ctaaaataac gcgattcgta ttcaggattt ttgttatgtc cattcgtcat acctcataaa
     8581 attctgacat taaatttcaa cgacccgaga tgtcgcatga tgtaattttt ccgtaatcac
     8641 catttcagtg acccgcttaa tatcggcatc attaaaaaaa gcggtaacat aagcgaccgg
     8701 catagtgact gtaggcaaat taatggtcga aataatcagt tcgatattgt caggcaaaac
     8761 ctgtgataaa ttcgctgctg aaataacatc aacaatcgtc cattcaggaa atgctcgcag
     8821 aatacggctt ttcagtagat gcgaagttcc gattccggtt gaacagacca gtaatacgcg
     8881 tttacgggca atttgccgct ccatcgcagc ctgaaaatga acggtcaggt aacctacttc
     8941 atcctcgctc actgcattct caccccaggt tttaaatacc tgattcacca catattgcgt
     9001 caaccgccag acatccgcta gttctgcttt aatatcttcc aacaatggat tacggatatg
     9061 aatacgataa tttagtcggt taatcagcgg tttaatatgc accaataagc catcatataa
     9121 tgcgctgtct tcactaaaat cgcagtccac gatgtcactg aataccgtaa ttaaacgcca
     9181 ggtaatcaga cgcgcttcat tgctggcctg catatgtgaa ataatgctaa catctttttg
     9241 tccatcaatc gccacacctg atgaaatgat atattgataa ataaaccaga cttcatcatc
     9301 gggcaatgta tgtgcaattc gttgttctat cttatgaatc atctgactgg caacattaaa
     9361 gatattttca tcatattgcc tggtttgatc ttcttctctt gataacgcat tcccgtgcgt
     9421 gttgcgatac atcataataa ggatgtgagt aaaaatgtta acgtaataag gttctcccaa
     9481 agaccaactt aattcatttt ccatatccag taacaatgac tggacaaata acacctcttc
     9541 ttctccaaaa taatggacta atgctttata gcttccggga tccagacgtg aatacacgac
     9601 accttgcggc tcattatggt taataatgcc gttaatcagt aatgccattg cctgtcggac
     9661 ttgcccttca ctaccttcaa tatgcgtacc acttggactg cggatcaacg ataaccccaa
     9721 cggcgcaagc caggactcaa ttactctcag gtcattaaca atagaggctc cgctgataaa
     9781 gtagcgttct gacaatttac tgatcgacgt ttcattcggt gtttcactta ataactgcga
     9841 cgctatttta acgcgcctgg aattattcat tacaccatca atctcttcac attctgccac
     9901 caacaaatga tcaagatgca gcaaatcagt aatattctcc gcgcttaaca tcacacccgc
     9961 gccagcacgc ttctcaacat ttattctcca ttgtcccagc cattgctcaa gtaaacgtaa
    10021 atcacgttga atggtttttt ccgaaacatt taaatagccg gcaatcgtaa caatagtcgt
    10081 atattccctt cgaggtaaaa ggaatcgtaa taatctgttt tgtcgcgagg taatcatttg
    10141 cattttttac ccttatcaca gattaagaag cctcatgagg aagagcttca gttgccatct
    10201 ctgagggtaa atataaagca gaataatttc acaacaatac taaaatctgt ccgtaatccg
    10261 gacagtgata aaacaaagca aggcggttaa gggatatttt tcagcacaga tcacattttc
    10321 gcgatgaata atcgtgatca tgctcgcaaa tacgatggaa gaaatattta ttaccagatg
    10381 tgagattatt taccggatac gacacaaaac cattcgtacc cggcattcca tcaatagatt
    10441 acaacgtaat tgtcagcgca ttcccttgag gcgtcaccat cagcccctgc tcactttcag
    10501 cctgcgaacc gccttgcaga ccatttactt tcacaatatt gcgcaagcac agtgtccagt
    10561 tcttcgcctc gcccgcacca gtcacagtaa tcgtgttacc agtacgtgtt gcttttagag
    10621 taaagatcac cgaaccgtca gcagcgggca tttcacagac ggcttcatgc ccgtcttgta
    10681 gattaaagag ctggaatgcc gtgccttcgt gccacgcgta atcgggacgt tgctcgttgt
    10741 tgcccagcgc cagtagggtg ttatcacgca cataaacggg cagactcagg aagctgtgct
    10801 gctgtttatg ccagcgacta ccatcgagtt catcgttgtg ccacaggtgt gtccagcgac
    10861 cttcaggcaa gtagaactgc acatcgcccg cttcagtgaa cacaggcgca accatcacgt
    10921 tgtcgcctaa catgtattga cggtcaaggt aatcacaagc cggatcgtcc ggaaactcca
    10981 tcatcatggc ccgcatcatc ggcgtacccc gcgcgttcgc acgagcagct tcacgataca
    11041 gatacggcat catgcggcat ttcagttgcg tgaagaagcg caccacatca caggactcat
    11101 catcgtaggc ccacggcaca cgataagatt tgctgccatg taaacggcta tggctggaga
    11161 gcaaaccaaa cgcgcaccag cgtttgtaaa catgcgccgg cgcggtattc tcaaagccgc
    11221 cgatatcgtg gctccagaag ccaaaacctg aaaggccaat agacaaacca ccgcgcaggc
    11281 tttccgccat tgattcgtag ttagcgtaac agtcgccacc ccagtgtacc gggaatttct
    11341 gcgcaccgac ggaagccgag cgggcaaaca agacagcttc ttcctcacca acggtgtcct
    11401 tgagcacgtt ccacaccagt tcgttgtaga tgtacgcata atggttatgc attttctgcg
    11461 gatcggaacc gtcaaaccat tgaacatcgg ttggaatacg ttcgccaaag tcggtcttaa
    11521 agcaatcaac gcccatcgcg accagacctt tcagtttgtc ggcgtaccat ttgcaggcat
    11581 ccggattggt aaagtcataa attgccagac ctggctgcca tttatcccac tgccataacg
    11641 aaccgtccgg gtgtttgagt aaatagcctt tctcttgtaa ctctttaaag acaggggatt
    11701 tttgcccaat ataggggtta atccagacgc agatttttag ccctttcgct ttcaggcggc
    11761 ggatcatccc ttccgggtcc gggaaagtca gcgggtccca ctcaaaatcg caccactgga
    11821 aggctttcat ccagaaacag tcaaagtgga aaacatgcag cggcagattg cgttccgcca
    11881 taccatcgat aaagctgttt accgtcgctt cgtcgtagtt ggtggtaaat gaagtggtta
    11941 gccacaggcc gaaggaccac gcgggcggca gcgccggacg accagtaaaa cgggtataac
    12001 gatcaagtac cgctttcggc gtcgggccgt cgataacaaa gtattcgaga tattcactct
    12061 caacgctgaa ctgcactttg gagactttct ccgatcccac ttcaaaagag acgcattgag
    12121 gatgattgac cagtacccca taaccacggt tagtcatata gaacgggata tttttatacg
    12181 cctgttcagt acttgtgccg ccgtcccggt tccaggtctc taccgtctgg ccattgcgca
    12241 ccagggcagt aaagcgctct cccagaccgt aaactgtttc gccaacgcca agatccagcc
    12301 gctcaaacat ataatttcgt tgattattcg tgtcctgcac gtagccatta tttttcacct
    12361 gactaccggt aatacgttcg ccgttgcgca gaaaatccag tgaccagaac tcacctttgc
    12421 tgacacgcgc gcttaagttg ccacttttaa actcagcata acgttctgtg ttttcgattg
    12481 tgaccttcac atcctgcaaa atattgagcg gataatgagg accgttattc agcgccccct
    12541 gaaaatgctc aatccgtaca ccaacaatac cttcctgtgg ggagaaaaag cgcagcgtaa
    12601 ataaaggtgt atcaagctgc caggtacgtt cacgcacatc acggggggca gcatagacca
    12661 ccatttcatt accctgctgt tcaacctcga acacctgaag cgggtgaatc aaattgaggc
    12721 caggttgaat caaccagttt ccgtcgctaa ttttcattgc cgttccttag ttctgcactt
    12781 cttgagaggt gaattgttgc tggcaacggc gtttgccctg cgccagctgt tccataacgg
    12841 ttttcagatt gtgcgtcgtg agtgagtagt agcgtttagc gataatcgcg ctcagcaagt
    12901 aacagatcgc cggaacaatc gtgaatagcg cgataatgat gctaatcgtg gcgctgttct
    12961 gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata agagcgccgc
    13021 cgaaggccaa ccccagcttg agcacaaaca gcgtgccagc aaaactgatc ccggtcaggc
    13081 gtttaccatt gcaccactcg ccgtagtcga cggtatcgga catcattacc cactggatag
    13141 gtgtcaccag ttgatgcaat acaccaatca cgaagatgaa gacaaacata gtgatgctgg
    13201 cctgcatggg aacaaagaac atcgcgaggc taatcactgc cagcagggcg ttcgtccacc
    13261 agaagatagt gactttacat ttccagtcgg tcagcggttt tgccagtgcg gaaccaatca
    13321 ggttacccac gcaataagtg gtgagaaaag cgacaaacac ttccggcgta cccaaaatcc
    13381 atgtgacgta atacatcatc gctccaccgc gtacgcacac cgccagaata ttgaaaatgg
    13441 ttagtaaacc gacaatccgc cactggtcgt tttgccagat atcacgtaaa tcttcccgca
    13501 tagacgtcgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa cagaatgcca
    13561 gcatcatgaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg agtggtttat
    13621 tatcaccacc aattaaatta accagcggca tcatcagcac agtagagagc atgcctcccg
    13681 cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg tcattggtga
    13741 ttacaccacc caatgcgcag taagggatat taacgatggt ataaagtaag gtaagtaggg
    13801 tgtaagtaat tgctgcataa atcatttttc cgttcatact gagatctggc gtgctatagg
    13861 ccagtacaca gacgatccca aatggcagtg cgccaaacag tacccacgga cgaaatttac
    13921 cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa atcgcatcca
    13981 gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca aaaatatcgg
    14041 tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg gcggcgtctc
    14101 ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc tgcttttcct
    14161 tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct gcgagagcga
    14221 atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt ggtagcgtta
    14281 atttcgctca tatatcattg taaaatatga gttttatatg aactataatg ttttcgtgat
    14341 aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa ctcaaggaag
    14401 tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg tcgtctccgg
    14461 tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca ggttcgactc
    14521 ctgtgatctt ccgccaatta acatcttctg aactctcccg atgtcaatcc cccccttcta
    14581 atcccttgta tatcaggatt cctttgtatc cagaagtcaa ccgacttcac cccgtatcaa
    14641 gttgcttttg ggggtacttt agggggtatt atttcctatt gaaatatcag cacccccata
    14701 attggtaaaa tatgatatat aacaacggct ttcaacaaca tctttgttat acaaggggtg
    14761 cattgggggt atcaggttaa ttactggcag aaatccccct caggaacatg aattcattaa
    14821 aagccggagg atgtcatggc attaaccgat attaaagtga aaaccgcaaa accaaaagaa
    14881 aagccctata aacttgctga tggcggcggt atgtatctac tgattaacac taatggttct
    14941 aaatactggc gtatgaagta ccgctttgct ggtaaagaaa agatgctttc tattggcgta
    15001 taccccgatg taacgctagc cgacgctcgt gaaaaacgca gcgaagccag aaaaatcctc
    15061 gctgctggag gcgatccagg ggtggcaaaa aaagaagaaa aaatcgcgca gcaaatgagt
    15121 ttgaaaaata cgttcgaggc cattgctcgc gaatggcatc agtcaaaagc cgatcggtgg
    15181 tcattacgct atcgtgatga aatcattgat acctttaaaa aagacatttt tccctacatt
    15241 ggcaaacgcc ctatcgccga aatcaaacca atggagttac tggaagcact gcgtaaaatg
    15301 gaaaaacgtg gagctctgga gaaaatgcgc aaagtccgcc agcgctgtgg tgaggtgttc
    15361 cgctatgcaa ttgttactgg tcgggcagac tacaaccctg ctcccgatct tgcctgtgct
    15421 ttagctacac cgaaaaaagt acattttccc tttcttactg ctaatgaact tccccacttc
    15481 ctcaacgatc tggcgggtta taccggaagt atcatcacta aaacagctac tcagatcatt
    15541 atgttaaccg gtgtacgaac acaagaatta cgttttgcac gctgggagga tatcgatttt
    15601 aagacaaaat tatgggagat tccggcagaa gtaatgaaga tgaagcgacc tcatattgtg
    15661 ccgctctctg agcaggtcat tgccctattt aagcaacttg aacccatcac aaaacatcat
    15721 cctctggtct ttatcggtag gaacgatcct cgcaagccaa ttagtaagga aagcattaac
    15781 caagtcattg aattactggg atataaggga agactcacag ggcacggttt cagacacacc
    15841 atgagtacca ttctacacga acagggcttt aattccgctt ggattgaaac gcagttagct
    15901 catgtagata aaaacgctat cagggggacc tataatcatg ccctgtatct agatggtcgc
    15961 cgtgaaatga tgcaatggta cgctgattac attgattcgc tttccagcca cgagagtcag
    16021 tgtcaaggcc ccttgtactg atattttctc tcaattcaca aaattcctcc ggcctccagg
    16081 tctggaggaa ttttgttttc atgttttatt tttagatatt ttttgacaaa atatttatat
    16141 tgcatctgca aacaatctca gaacatcaca ccgctataga ccgctaacaa ttttgatgcc
    16201 tgtaatttca atcatcatcc tgtcgttatt tcagcaccat cacaccaaaa atcaactaac
    16261 atgttgtctt agccgactat tcacaatgca ttggaatgca gataaagata aatttttatc
    16321 cacatgaaga attactgtcg atgatattgt aaacaatatt catcccgggc aataacaaga
    16381 cttgatacac aacatcacga cttttatgct tttcagattg tgtcccatcc gttttacagg
    16441 ctcagaaaat gaacgacaga ttacgctttg atgtgaacga taaccaaggc cgtttcgtat
    16501 ttccgaatac caggtttggc ccattactgg aggagtttga agaagtgcat gacgcatacg
    16561 gcactgatga aatcagcgag acgagttata tcaacaaact caggtagctt gcccaacggg
    16621 agcctgactt tattgatata cacgctcatc tggcatatgc atttctggag cagccaaggt
    16681 gggcaatatt tttatttatt tctgtgaaaa gcctgctaaa gatcccatgc ttctatatta
    16741 atgactggaa caatactagg aagcacatta agaaaaaata aaggaagccg atggctacca
    16801 cgtttatcgt ttagaagcgt gagctcttca ttattaagcc ggacacttac aaaatgaagt
    16861 tgcttctg
//
LOCUS       DAZKGF010000107        16829 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZKGF010000107 DAZKGF010000000
VERSION     DAZKGF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.107"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(25..720)
                     /gene="araD"
                     /locus_tag="ACHOQV_003282"
     CDS             complement(25..720)
                     /gene="araD"
                     /locus_tag="ACHOQV_003282"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9631606.1"
                     /translation="MLEDLKRQVLEANLALPKHNLITLTWGNVSAVDRERGVLVIKPS
                     GVDYNVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(920..2422)
                     /gene="araA"
                     /locus_tag="ACHOQV_003283"
     CDS             complement(920..2422)
                     /gene="araA"
                     /locus_tag="ACHOQV_003283"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP9631607.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGIKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(2433..4133)
                     /gene="araB"
                     /locus_tag="ACHOQV_003284"
     CDS             complement(2433..4133)
                     /gene="araB"
                     /locus_tag="ACHOQV_003284"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFP9631608.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIANKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            4472..5350
                     /gene="araC"
                     /locus_tag="ACHOQV_003285"
     CDS             4472..5350
                     /gene="araC"
                     /locus_tag="ACHOQV_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFP9631609.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAAKLS"
     gene            5436..6200
                     /gene="yabI"
                     /locus_tag="ACHOQV_003286"
     CDS             5436..6200
                     /gene="yabI"
                     /locus_tag="ACHOQV_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9631610.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLAPLISAVGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(6314..7012)
                     /gene="thiQ"
                     /locus_tag="ACHOQV_003287"
     CDS             complement(6314..7012)
                     /gene="thiQ"
                     /locus_tag="ACHOQV_003287"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFP9631611.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERSEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTDELL
                     SGKASASAILGITG"
     gene            complement(6996..8606)
                     /gene="thiP"
                     /locus_tag="ACHOQV_003288"
     CDS             complement(6996..8606)
                     /gene="thiP"
                     /locus_tag="ACHOQV_003288"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFP9631612.1"
                     /translation="MATRRQPLIPGWLIPGISAATLVVAVALAAFLALWWNAPQGDWV
                     AIWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVLVDGINRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            complement(8582..9565)
                     /gene="thiB"
                     /locus_tag="ACHOQV_003289"
     CDS             complement(8582..9565)
                     /gene="thiB"
                     /locus_tag="ACHOQV_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFP9631613.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(9596..9695)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(9729..11384)
                     /gene="sgrR"
                     /locus_tag="ACHOQV_003290"
     CDS             complement(9729..11384)
                     /gene="sgrR"
                     /locus_tag="ACHOQV_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFP9631614.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAA
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQILPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     SSNQLKIQAFDDFFGYRALIDEVNVWVLPEIADDPAGGLMLKGPQDEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHTRT
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEINLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            11473..11604
                     /gene="sgrT"
                     /locus_tag="ACHOQV_003291"
     CDS             11473..11604
                     /gene="sgrT"
                     /locus_tag="ACHOQV_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFP9631615.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(11712..12317)
                     /gene="leuD"
                     /locus_tag="ACHOQV_003292"
     CDS             complement(11712..12317)
                     /gene="leuD"
                     /locus_tag="ACHOQV_003292"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFP9631616.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVQANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(12328..13728)
                     /gene="leuC"
                     /locus_tag="ACHOQV_003293"
     CDS             complement(12328..13728)
                     /gene="leuC"
                     /locus_tag="ACHOQV_003293"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFP9631617.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(13731..14822)
                     /gene="leuB"
                     /locus_tag="ACHOQV_003294"
     CDS             complement(13731..14822)
                     /gene="leuB"
                     /locus_tag="ACHOQV_003294"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFP9631618.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVVEGV"
     gene            complement(14822..16393)
                     /gene="leuA"
                     /locus_tag="ACHOQV_003295"
     CDS             complement(14822..16393)
                     /gene="leuA"
                     /locus_tag="ACHOQV_003295"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFP9631619.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(16486..16572)
                     /gene="leuL"
                     /locus_tag="ACHOQV_003296"
     CDS             complement(16486..16572)
                     /gene="leuL"
                     /locus_tag="ACHOQV_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFP9631620.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRLVSGIQN"
BASE COUNT         3711 a         4582 c         4584 g         3952 t
ORIGIN      
        1 attggctgtg gttttataca gtcattactg cccgtaatat gccttcgcac catgcttacg
       61 cagatagtgt ttatccagca gcgtttgctg catatccggt aactgcggcg ctaactgacg
      121 gcagaatatc cccatataag cgacctcttc cagcacgatg gcgttatgca ccgcatcttc
      181 ggcatttttg ccccatgcaa atgggccgtg agaatggacc aggacgccgg gcatttgtgc
      241 tgcgtcgatt ccctgttttt cgaaggtttc tacgatgacg ttaccggttt cccactcata
      301 ttcaccgttg atttctgcgt cggtcatttt gcgggtgcag ggaatggtgc cgtagaaata
      361 gtcggcgtgg gtggtaccgg ttgccggaat tgactggccc gcctgcgccc agatggtggc
      421 gtggcgcgag tgtgtgtgca caatgccgcc aatggacggg aatgcctgat agagcagccg
      481 gtgagttggc gtgtcggagg agggcttttt cgttccttca accacttcac cggtttcgat
      541 gctgaccacg accatatcgt cagcggtcat gacgttgtaa tcgacgccgg aaggtttgat
      601 caccaagacg ccgcgctcgc gatcaacggc gctgacattg ccccatgtga gcgtgatcag
      661 gttatgtttt ggcagcgcca aattggcttc taatacctgg cgtttgagat cttctaacat
      721 gttgactcct tcgtgccgga tgcgctttgc ttatccggcc tacaaaatcg cagcgtgtag
      781 gcctgataag acgcgtcagc gtcgcatcag gcggtgaatg ccggatgcgc tttgcttatc
      841 cggcctacaa aatcacagcg tgtaggcctg ataagacgcg tcagcgtcgc atcaggcgtt
      901 acacacctga tgcggctact tagcgacgaa acccgtaata cacttcgttc cagcgcagcg
      961 cgtctttaaa cgctggcagg cgggtatcgt tatcaatcac cgtgatttca atgtcgtgca
     1021 tctcggcgaa ctggcgcata tcgttgaggt tcagcgcatg gctgaaaacg gtatggtgcg
     1081 cgccaccagc aaggatccac gcttcggaag cagttggcag atccggttgc gctttccaca
     1141 gcgcattcgc caccggcagt ttcggcaggg agtgcggtgt tttcaccgtg tcgatgcagt
     1201 taaccagcag acggtaacga tcgccgagat caatcaggct ggcgacaatc gctggaccgg
     1261 tttgggtatt gaagatcagg cgggcaggat cgtctttacc gccaataccg aggtgctgaa
     1321 cgtcgaggat cggtttctct tccacagcga tcgacggaca gacttccagc atgtgggagc
     1381 cgagcaccag gtcattgcct ttctcgaagt gataggtgta gtcctccata aaggaggtgc
     1441 cgccctgcag accggttgac atcaccttca tgatgcgaag cagggcggca gttttccagt
     1501 cgccttcgcc cgcaaagccg taaccctgct gcatcagacg ctgtacggcc agaccaggaa
     1561 gctgtttcag accgtgcaaa tcttcaaagg tggtggtgaa cgcgtggaag ccaccttgtt
     1621 ccaggaaacg cttaatcccc agctcaatac gcgccgcttc cagcacgttc tggcgttttt
     1681 cgccgtggat ttgtgtggca ggcgtcatgg tgtagcagct ttcgtactca tcgaccagcg
     1741 cgttaacatc gccatcgctg atggagttca ctacctgcac cagatcgcca accgcccagg
     1801 tattgacgga gaaaccgaac ttgatctgtg cggcaacttt atcaccatcg gtgaccgcca
     1861 cttcacgcat gttatcgcca aaacggcaga ctttcagatg acgggtatcc tgtttagaga
     1921 ccgcctgacg catccaggag ccgatacgct catgggcttg tttatcctgc cagtgaccgg
     1981 taacgacggc atgttgctga cgcatacgcg cgccaatgaa gccgaactcg cgaccgccat
     2041 gtgcagtctg gttcaggttc ataaagtcca tatcgatgct atcccacggc agcgccgcgt
     2101 taaactgggt gtggaattgc agcaacggtt tgttgagcat agtcaggccg ttgatccaca
     2161 ttttggccgg ggagaaagtg tgcagccaca ctaccaggcc agcgcaacga tcgtcgtaat
     2221 tcgcgtcacg gcaaatagcg gtgatttcat ccggcgtggt gcccagcggt ttcagcacca
     2281 gtttgcaggg cagtttcgct tccgtattca gcgcattaac gacgtgctcg gcatgttggg
     2341 tgacctgacg cagggtttcc gggccataca gatgctggct gccaattaca aaccacactt
     2401 cataattatc aaaaatcgtc attgtcgtgt ccttatagag tcggaacggc ctgggcagcc
     2461 tgtgccgggg cggaagttgg aagatagtgt tgttcggcgc tcatcgccca ttgctgatag
     2521 cggcgataaa gctgttcaaa gcgttgtgcc tgttcgctgc gcggttgcag ggttttctct
     2581 accgcactgg ccattttttg ctgggctgag gggatgtctg cgtgcacttt cgcggcgacg
     2641 gcagcgaaaa tcgccgcacc gagcgcacag cactggtcag aggcaacaat ttgcagcggg
     2701 cgattcagca cgtcgcagca ggcctgcata atgacctggt ttttccgcgc gatgccgccc
     2761 agcgccatca cgttattgac ggcgatcccc tgatcggtaa agcactccat gattgcgcgt
     2821 gcgccaaagg cggtggcagc aatcaatccg ccgaacagca gcggagcgtc ggtagcgagg
     2881 ttcagatcgg taatcacccc tttcaggcgt tggttagcgt ttggcgtgcg gcggccgtta
     2941 aaccagtcga gcaccaccgg caggtgatcc agagacggat ttttggccca tgcttcggtc
     3001 agcgccggaa gcagttgttt ctggctggcg ttgatttgct ctttcagttc cggatgctgg
     3061 gcggcaagct gttccagcgg ccagccgagt acgcgaccga accaggcgta gatatcacca
     3121 aacgccgatt ggcctgcttc cagaccgata aatccaggca ccacgctgcc atcaacctga
     3181 ccgcaaatac ctttcactgc ccgctcgcca acgctctgtt tgttggcaat cagaatgtcg
     3241 caggtggaag taccgataac ttttaccaga gcgttaggct gtgcgcctgc gccaactgcg
     3301 cccatatggc agtcaaacgc gccgccggaa atcaccacgc tttcaggtag gccgagacgc
     3361 tgcgcccatt ccgggcataa ggtgcccacc ggaatatcgg cagtccaggt gtcagtgaat
     3421 agcggggaag gcaaatggcg attgaggatc gggtccagct catcaaagaa actggctggc
     3481 ggcaggccgc cccagctttc atgccatagc gacttatgcc cggcgctgca acgtccgcga
     3541 cgaatatcct gcgggcgggt ggtaccggaa agcagagctg gcacccagtc gcacagctca
     3601 atccacgatg cggcagattg cgccacggcg ctgtcctggc gagtcacatg cagtattttt
     3661 gcccagaacc attcgctgga ataaataccg ccaatatagc gggagtagtc aacgttgccc
     3721 ggcgtgtgac ataagcgggt tatctcttcc gcttcttcaa ccgcggtgtg gtctttccac
     3781 aatacgaaca tcgcgttcgg gttttcggca aactccgggc gcagcgccag gacattaccg
     3841 tccgcgtcaa tcggtgcggg cgtcgagccg gttgtgtcaa cgccaatccc gaccacagct
     3901 gcgcgctgct cggcgctaag ctctgcaagc acggttttca gcgccgcttc cattgactca
     3961 atgtagtcac gcggatgatg acggaactgg ttatttgggg catcacaaaa ttgccctttc
     4021 tgccaacggg gataccactc tacgctggtg gcgatctctt caccggtggc gcagtccacc
     4081 gccaaagctc gcacagaatc actgccaaaa tcgaggccaa ttgcaatcgc catcgtttca
     4141 ctccatccaa aaaaacgggt atggagaaac agtagagagt tgcgaaaaaa agcgtcaggt
     4201 aggatccgct aatcttatgg ataaaaatgc tatggcatag caaagtgtga cgccgtgcaa
     4261 ataatcaatg tggacttttc tgccgtgatt atagacactt ttgttacgcg tttttgtcat
     4321 ggctttggtc ccgctttgtt acagaatgct tttaataagc ggggttaccg gttggattag
     4381 cgagaagagc cagtaaaaga cgcagtgacg gcaatgtctg atgcaatatg gacaattggt
     4441 ttcttctctg aatggcggga gtatgaaaag tatggctgaa gcgcaaaatg atcccctgct
     4501 gccgggatac tcgtttaacg cccatctggt ggcgggttta acgccgattg aggccaacgg
     4561 ttatctcgat ttttttatcg accgaccact gggaatgaag ggttatattc tcaatctcac
     4621 catacgcggc cagggggtgg tgaaaaatca gggacgagaa tttgtctgcc gaccgggtga
     4681 tattttgctg ttcccgccag gagagattca tcactacggt cgtcatccgg aggctcgcga
     4741 atggtatcac cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg
     4801 gccgtcaata tttgccaata cgggtttctt tcgcccggat gaagcgcacc agccgcattt
     4861 cagcgacctg tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct
     4921 gctggcgata aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc
     4981 gctccatcca ccgatggata atcgggtacg cgaggcttgt cagtacatca gcgatcacct
     5041 ggcagacagc aattttgata tcgccagcgt cgcacagcat gtttgcctgt cgccgtcgcg
     5101 tctgtcgcat ctgttccgcc agcagttagg aattagcgtc ttaagctggc gcgaggacca
     5161 acgcattagt caggcgaagc tgcttttgag caccacccgg atgcctatcg ccaccgtcgg
     5221 tcgcaatgtt ggttttgacg atcaactcta tttctcgcga gtatttaaaa aatgcaccgg
     5281 ggccagcccg agtgaattcc gtgccggttg tgaagaaaaa gtgaatgatg tagccgccaa
     5341 gttgtcataa ttggtaacga atcagataat tgacggcttg acggagtagc atagggtttg
     5401 cagaatccct gcttcgtcca tttgacaggc acattatgca agcattgctg gaacacttta
     5461 ttacccaatc caccgtgtat tcattgatgg cggtggtgtt ggtagccttt ctggagtcgc
     5521 tggcgctggt cggtttgatt ctacccggta cggtgctaat ggcgggactg ggggcgctga
     5581 ttggcagtgg cgagttaagt ttctggcacg cctggctggc agggatcgtc ggttgtctgc
     5641 tgggcgactg gatctccttc tggctgggct ggcgttttaa aaagccgttg catcgctggt
     5701 catttctgaa gaagaacaaa gcattactcg ataaaaccga acatgcgctg catcagcaca
     5761 gcatgttcac cattctggtc ggtcgctttg ttggcccgac gcgtccgctg gtgccaatgg
     5821 tggcgggaat gctggatctg ccggtggcaa aattcattac gccgaatatt atcggctgcc
     5881 tgctgtggcc accgttttac ttcctgccag ggattctggc gggtgcggcg atcgatattc
     5941 cagccggaat gcagagcggc gagtttaaat ggttgctgct ggcgacggcg gtatttttgt
     6001 gggttggtgg ctggctgtgc tggcggttat ggcgcagcgg taaagcgact gaccgtttga
     6061 gtcattattt gtcgcgcggt cgtttgttgt ggctggcacc gttaatttct gccgtcggtg
     6121 tagtggcgct ggtggtgtta attcgccacc cgttgatgcc ggtgtatatc gatattttgc
     6181 gtaaagtggt tggggtttag gggatagcct tgtgcgggtt gcctgatgcg acgcttgccg
     6241 cgtcttatca ggcctacaaa acgcactgtc cgtaggtcgg ataaggcgtt cacgccgcat
     6301 ccgacagtgc atactaaccc gtaatcccca atattgccga agcactcgcc ttaccgctca
     6361 acaactcatc ggtcataccc tgccaggcga tgcgcccgtc ggcgaccacc accgagcgcg
     6421 tggcgatccg cgccgcatct tccacactgt gcgacaccat caatagcgtc attttttgct
     6481 gttggcagct cgtgctcacc agcgtcaaca tctcctgacg taacgccgga tcgagcgcag
     6541 agaacggttc atcgagcaat aaaatcggct gttcgcgcac cagacaacgc gctaacgcca
     6601 cgcgctgtcg ctgaccgcca gaaagctcgc ccggtaagcg cgccattaaa ttatcaatcc
     6661 ccatctgacg ggcaatcgcg tgcattttct cctgttgtgc cgcgttcagc ttcaatcctg
     6721 gattaagccc cagcccgatg ttctgcgcga ccgtcaggtg actgaacagg ttgttttcct
     6781 gaaacagcat cgacaccgga cggcgtgacg gcggcgtcgt ggtgtgatca acgccgtcga
     6841 tagtcagcga accgctggct ggcgtcagaa aaccggcgat caaattcagc agggtacttt
     6901 tacccgcgcc gcttggcccg aggatcgcca cctgctcgct gcgttccacc gttaagctaa
     6961 aacgcatcgg caaatggtgg taaagccagg tgatatcagt cagttttaac atttcgcccc
     7021 ggtagttttt caatcacggt aaacagcaga aaacagagta gcagcagaat taacgcggtg
     7081 accgcgccgt cctggctgcg ataggagcca atttgctggt agagataaaa cggcagggtg
     7141 cggaaatcat cgttaccaaa caacgccacc acgccaaaat caccaatcga tagcacacaa
     7201 gcaaaggcca gtgcctgcgc cagtgggcgt ttaagggcgc gcagctccac cacttttaag
     7261 cgtgaccagc cttcaatgcc cagcgactga cacaacatac tgtaacgcgc ggtgatatcg
     7321 cgcatcgggt tttccagcac tttcagcgca taagggatcg ccattaacgc attggtgaaa
     7381 atcacaatgc cgtcagcaga ttgtggcagg ccgatagtgt tgttgagcag taaaaagaag
     7441 ccggtagcca gcacaatccc cggcatcgcg aggatcaaca tgccgctcat ctccagcgcc
     7501 tgacccgcca gcattttctg ccgcgcccgc agttcgcgac tgctccatag cagcatcatg
     7561 gtcagcacta cgcataatac gcctgccgcc agcgcaatac gcaacgaggt ccacagcgcc
     7621 tgccacagca ccggctgcgc cagcacttcc ggcaactggc gatttatccc atcgacgagc
     7681 accgccagta acggtggcag caacagcaac agcgccagca caattaacac cgtgtcgcaa
     7741 atgcggctat gcagacgatc gtccgggtcg cgccagcctt gcagcagcgt agtgccgggc
     7801 gcaatggcct tactcaatcg ctgactcaac agcaccagcc caaggcaaca caccatctgg
     7861 atcagcgcca gcatcgccgc gcgggcagga tcgtagtcgt aactcagcgc ctgataaata
     7921 gccagctcga tagtcgtcgc ttgcggaccg ccgcccagcg acaacacggt ggcgaagctg
     7981 gcgaaacaga gcataaagat aagcgcggca accggcggga tttgtcgccg taaccacggc
     8041 cattcgacga agcggaaaaa atgccagccg cgcatcccaa gctgggcggc aagctgacgt
     8101 tgttccccgg gaatgttttc cagtgcctgg agtaataagc ggctcgccat cggcagatta
     8161 aaaaatacat gcgccagcaa aatgccttgc aggccgtagg gcgaaaacgt ccactccaga
     8221 ccaagcgatt gccagagcga tgccagccag ccctggcgac catagacgct aagaatgccg
     8281 aaaacagcaa ccagcaccgg gaggatcaag gtcatggcac acagacgcaa cagcgccagc
     8341 cgtcctggaa agcgcctgcg gtagagggcg cgggcaagga atatcgcggg cacgacagag
     8401 agcagcgccg agagaaacgc ctgccagaag gagaagcgca ccacatgcca caggtagctg
     8461 tcctgccaga ttgccaccca gtcaccctgc ggcgcgttcc accacagggc gagaaacgca
     8521 gccagtgcaa ccgccaccac cagcgtggcg gcgcttattc ctggaattaa ccagccggga
     8581 attaacggct gacggcgcgt tgccattcgc taatccatgc ctgacgttgt gccgccactt
     8641 cggctggcgt gaactccagc gtggttgccg gtttggtcaa ttgttcaaaa ccggcaggca
     8701 gcgtgacgtt tgccaccgga tacatccagt tgccggttgg gatcgcattc tgaaaagccg
     8761 gagaaaccat aaactggaga aatttttgcg ccagctccgg ctgcttgctg gcagcggtgc
     8821 gggcggcgac ttccacctgc agatagtgac cttcgctgaa gtttgcggcg gcgtagttat
     8881 ctttcttctc ttcgagaatg tgataagccg gagaggtggt gtaactcagt accagatcgc
     8941 tttcaccttt taaaaacagg ccgtaggctt cgctccagcc tttggtgacc gtgaccgttt
     9001 tcttcgccag tttctgccag gcttgtgggg cgtcatcgcc atagactttt tgcatccaca
     9061 acagcaggcc cagccccggc gtactggtgc gcggatcctg ataaatcacc cgccagtttt
     9121 gatcgctctc aacaagctct ttcaggcttt gcggcggatt tttcagtttg ttcttgtcat
     9181 agacgaaggc gaaatagccg taatcaaacg gtacgaaagt gtcatttttc cagccgccgg
     9241 gaacgttaac ggcatccgct gccacgccgc ttttggcaaa tagtccggtt ttactggcgg
     9301 cgtctaacag gttgttatcc agccccagca ccacatcggc tttactgttt ttgccttcca
     9361 tccgcagacg gttgagcagc gaaacgccat cttccagcgc caccagtttc agttcgcagt
     9421 tacagtcggc ttcaaaagct tttttgatct ttggaccagg cccccagtcg gcggcgaagg
     9481 aatcgtaggt ataaacagtc agaacgggtt tagcgaaaac gggcgctgtg cacagcaaca
     9541 gcaaggggag acattttttt aacactttgc acctcaaaaa agagtggcaa aggacttgag
     9601 aaggagcctc aaatcccttc gccggcgtta tccggatcag gttcgacggg tattttctca
     9661 gcgcacgcgt ctgcgtggca ccccgttgag aacggcgtta gtgtagtgat tttgttatca
     9721 accagcaatc atggatccgg cggcgcaaac cacgctgatt taaaatcgaa ccagccgagg
     9781 gtattcatac gcaggccgcg catactgcgt tgcccctgaa tgatcagcca gtggtggata
     9841 agcggcacca ttgctttgct ggcgaccagt tgctggcacc agttcgccag gtttatttcg
     9901 ccattgcgcc agcgggcggc gtcggcttgc cagtcgatcg gaatacagtg ttgtagcagc
     9961 ggcacctcgc acaggtgcgc gaatagcgaa aaatccagcg gcagcgtaaa gttggcgctg
    10021 ttaagccaga tatcgctctc gatctctcct tcatgccact gatcgtagct gatctctttg
    10081 atttccagcg tgacctggtg acttgccaga atctgctgca tgatcccggc aatcacccta
    10141 tgctcactgt gatcctgata aaaggtcagg gtgaggcttt ccagaccagc cggtttttcg
    10201 ctctttatgg tgcgggtatg gtgccaacgg ggaagcagcc cataggccgg aaaccacaac
    10261 tgctggtact gttcctcggc gaaataaacc agattggtcg gcgaaagcac atagcttatc
    10321 cagtctctga cttgctgatt cgccccgcga tgggtacggc tgtcgaacag taaatagtaa
    10381 caaccttctt caaggcggct ttcaatctct ttttcctcgt cttgcggtcc tttcagcatc
    10441 aggcctcccg cggggtcatc agcaatttcc ggcaagaccc agacgttaac ttcgtcgatt
    10501 aatgcccgat aaccaaagaa gtcgtcgaat gcctgaattt tcagttgatt gctgctgttg
    10561 cggatcaccg cataaggacc cgttccgatg ggatggctgg caaagttgct gagggtttcc
    10621 cattcgcgcg gcaggatcat cgccggaact tgccccagca gtaacggtaa ccagcggtcc
    10681 ggttgcgtga gatggatatc cagcgtccag ggcgtcggcg acacaatgtc agcaatatgc
    10741 gaatagagcg gcagcgtatt gattcgtttt aaagaggcga tcacgtcgtc catttccagt
    10801 tcacgaccat gatgaaaatg gactcctgga cgcaaaaaga aacgccagtg aagcggtaaa
    10861 atttgctgcc agtggtgggc gatgtccgct tccagttccc cattttcctc atttatgcgc
    10921 gttagcgaac tgaagatttg ccgggcgata tgggtttcgg aacgacgcaa tgcactgccg
    10981 ggcagcagat tacgcaacgg acgatagtag agaacgcgca ggatgtgccg cccctggcgg
    11041 aagctgcgtc ccagatgaga aaccaacatt tgccgcacag ccgctttgtc gccaaccagt
    11101 tgcaccagtt gatcgatacg atcctgctcc agcaggtcct ctgctcgctg ttgctgaagc
    11161 gccagcccgg tatagaggaa tgtcagacgc gagcgtttgc cgcgcccgac ttccgcttcc
    11221 cacgtcagcc agccgcgatc ctgcatggtg ttgagcaggg tgcgcatatg acgacgcgag
    11281 cagctcaata acgctgccag ttcgttgagc gtcgtgtcct gcgatttacc ctcgcagcat
    11341 tgccacaggc ggatgaactg ttgttgcaga cgagcagatg gcataaaagg ggaactcctg
    11401 cgcaaaagac agcaatttta ttttccctat attaagtcaa taattcctaa cgatgaagca
    11461 aggaggtgcc tcatgcgtca gttttatcag cactatttta ccgcgacagc gaagttgtgc
    11521 tggttgcgtt ggttaagcgt cccacaacga ttaaccatgc ttgaagaact gatgcaatgg
    11581 gaggggagtc attctgacta ctgatttgcg gacatcatgt gtgactgagt attggtgtta
    11641 atcgccacgc cagcagtgat tatctgctgg cttttttctt tttggcgatt gagtgccgga
    11701 cggtgtacaa actaacgcat aaacgccggt tgcttctctt cataagacgc aattgcgtcg
    11761 tcgtgctgca gagtcaggcc gatgctgtcc agaccgttca tcatgcagtg gcggcggaag
    11821 gcatcgatgg taaagcgata agttttctct cccgctttca cctcttgcgc ttccaggtct
    11881 acatcaaaat gaatccccgg attggcctgc accagcgcaa acagttcgtc cacttctgcg
    11941 tcgcttaatt tcaccggcag cagctggttg ttaaagctgt tgccgtagaa gatgtcagca
    12001 aaactcggtg caatcaccac tttaaaaccg taatcggtca atgcccacgg cgcgtgctca
    12061 cgcgaggaac cgcagccgaa gttttctcgt gccagcaaaa tggaagcgcc ctgatactgc
    12121 gggaagttca gcacgaagtc cgggtttggc tgttggcctt tttcatccag aaaacgccag
    12181 tcgttaaaca gatgcgcgcc aaaacccgta cgggtcactt tctgcaaaaa ctgtttcggg
    12241 atgattgcat cggtatcgac attggcggca tccagcggaa ccaccaggcc tgtgtgtttg
    12301 ataaatttct ctgccatggt gtgctcctta tttaatgttg cgaatgtcgg cgaaatgtcc
    12361 ggtcacagca gcagcggcag ccattgccgg gctgaccaga tgggtgcgcc ctccacgccc
    12421 ctggcggcct tcaaagttac ggttgctggt ggaggcacaa cgctcacccg gattcaggcg
    12481 atcgttgttc atcgccagac acattgagca acctggcaag cgccattcaa aaccggcttc
    12541 aataaagatt ttgtccagac cttccgcttc cgcctgggct tttaccgggc cagagccggg
    12601 aaccaccagt gcctgcacgc ctggcgcgac tttccgtcct ttggcgattt cagctgccgc
    12661 gcgtaaatct tcaatacgtg agttagtgca ggaaccgata aacactttgt cgatagccac
    12721 ttctgtcagc ggaatacccg gtttcagccc catataggcc agcgcttttt ctgccgacgc
    12781 gcgttcaacc ggatcggcaa acgaagccgg atcgggaatg ttgtcgttca cggaaatcac
    12841 ctgacctgga ttggtgcccc aggtgacctg cggtgaaatt tcttctgcct gcaaagtcac
    12901 aacggtatcg aaagttgcgc cttcgtcggt ttgcagagtt ttccagtagg caacggcgtc
    12961 gtcgaaatct ttgcctttcg gcgcatgcag acggcctttg acatagttaa aggtagtttc
    13021 gtccggtgca accagaccgg ctttcgcgcc catttcgatt gccatattgc acagggtcat
    13081 acgaccttcc atgcttaaat cacggattgc ttcgccgcaa aactccacca catgcccggt
    13141 gccgcctgcg ctaccagttt taccgataat cgccagcacg atatctttcg ctgtaatgcc
    13201 cggcgcggct ttgccctgga cttcaatttt catggtcttc gcacgaccct gtttcagggt
    13261 ttgcgttgcc agtacgtgtt caacttcgga agtgccgata ccaaaggcta gtgcgccaaa
    13321 cgcgccgtgg gtggcggtat gcgagtcgcc gcagacaatg gtcatccccg gcaatgtgac
    13381 gccctgttcc ggccccatta cgtggacgat cccctgatac gggtgattca ggtcatacag
    13441 ctcgacgcca aattctttgc agtttttgat cagctcctgc atctggatgc gcgccatttc
    13501 accgcaggca ttaatgtctt tggtctgagt agagacgttg tgatccatag tggcgaaggt
    13561 tttgcctggc tgacgtaccg ggcgaccgtg ggcgcgcaga ccatcgaacg cctgcggtga
    13621 ggtcacttca tgcaccaggt ggcggtcgat atataacagc ggggtttcgt tttcggcttc
    13681 gtacactacg tgagcgtcga acaatttttc gtataacgtt ttagccatga ttacacccct
    13741 tctaccacat agcgggcaat gatatcgccc atttcattgg tactaacggc ggcagcgcca
    13801 cgggctaaat ccccggtgcg aatgccttct tctaatgcgc ggttaatggc gcgttcaatg
    13861 gcgcaagccg catcatctgc atccaggctg tagcgcagca gcagtgccag cgacagaatt
    13921 tgcgcaatcg ggttggcgat gtttttgcct gcgatatctg gtgctgagcc gcctgccggt
    13981 tcatacaaac caaaaccttg ctcgttcagg ctggcggaag gcaacatccc catcgagcca
    14041 gtgatcattg cgcactcgtc agacagaatg tcgccaaaca ggttggaaca cagcaggacg
    14101 tcaaactgtg atggatcttt aatcagctgc atggtggcgt tgtcgatgta catatgcgcc
    14161 agttcgacat ccgggtattc cgtggcgatc tcgttgacga tttcacgcca taaaatagag
    14221 gattgcagca cgttggcttt atcgatcgac gtcactttat ggcgacgttt acgagcagat
    14281 tcaaacgcga tgcgggcaat acgctcgatc tcaaaacggt gatacacctc ggtatcaaac
    14341 gctttttcat attgtccgct accttcgcgg ccttttggct gaccgaaata gatgccgccg
    14401 gtcagttcac gcacacacag gatgtcgaag ccgttagcgg caatgtcagc acgcagtgga
    14461 cagaatgctt ccagcccctg atacagtttt gccggacgca ggttgctgaa taatttgaag
    14521 tgcttacgca gaggcagcag cgcgccgcgt tctggttgct ggtctggtgg taaatgttcc
    14581 cacttcggac cgcctaccga gccaaacagc acggcatcgg cttgctcaca accttcaacc
    14641 gtcgcaggcg gcagtggttg cccgtggtta tcaatggctg cgccgcctac atcgtaatgg
    14701 ctggtggtga tacgcatcgc aaatcggttg cgcacggcat ccagcacttt cagcgcctga
    14761 gtcatcactt ccggaccaat accgtccccc ggcaatacgg caatatggta attcttcgac
    14821 atcacacggt ttccttgttg ttttcgttgt gttgagcttt gcgttgcaac tctttttcga
    14881 cttctgcggc acgccagata ttgttcagta cgtgcaccat ggctttggcg gaggactcga
    14941 caatatcggt ggccaggccg acgccgtgga agcggcgacc gttgtagttg gcgacaatat
    15001 ccacctgacc cagcgcatct ttaccgtgac ctttggcggt caggctgtat ttcaccagtt
    15061 cgacgttata gtcagtgatg cggtttatcg cctggtagac ggcatcgacc ggaccgttac
    15121 cgttggcggc ttctgctttg acttcttcgc cgcaggccag tttgacggcg gcagtggcaa
    15181 tatcgttaga acctgactgc acgctgaagt aatccagacg gaaatgctcc ggctcttctt
    15241 gctgcttacc gatgaaggcc agcgcctcca gatcgtaatc aaacacctga ccttttttgt
    15301 ccgccagctt caggaaggca tcgtacaaat tgtctaaatt atattcactt tctttatacc
    15361 ccatctcatc catgcggtgt tttactgccg cacgccccga acgagaggtc agattcagct
    15421 ggatttggtt caaaccaata gattctggtg tcatgatttc gtagttttcg cggtttttca
    15481 gcacgccatc ctggtggata ccggaggagt gtgcgaatgc gccgctgcca acaatggctt
    15541 tgtttgccgg gatcggcata ttacaaatct ggctaactaa ctggctggtg cgccatatct
    15601 cctggtgatt aatagcggtg tggacgttga ggatatcctt acgaactttg atcgccatga
    15661 tgacttcttc cagcgaacag ttaccggcac gctcgccgat cccgttcatt gcaccttcca
    15721 cctggcgtgc accggcatgt accgccgcca gtgagttgcc gaccgccagg cccagatcgt
    15781 cgtgggtatg tacggaaata atggctttgt cgatgttggg tacgcgttca tacagcccgc
    15841 tgatgattcc ggcgaactca aatggcatgg tgtagcccac ggtgtccgga atgttgatgg
    15901 tggtggcacc ggcattaatc gccgcttcga ccactcgcgc cagatcggca attggggtgc
    15961 gtccggcatc ttcgcaagaa aattcaacat catcggtgta attacgggcg cgtttcacca
    16021 tatagatagc gcgttcgatc acttcgtcca gcgtgctgcg cagcttggtg gcgatgtgca
    16081 ttggcgaagt ggcaataaag gtatgaatac ggaaggcttc ggcgactttc agggattcgg
    16141 ctgccacgtc aatatctttt tccacgcagc gagctaacgc gcatacacgg ctatttttaa
    16201 cctggcgagc gatggtctgc actgattcaa aatcacccgg cgaagagacg gggaaaccga
    16261 cttccatcac gtcaacaccc atacgctcaa gggccagcgc aatttgcagt ttttctttca
    16321 cactcaagct tgcctgtaac gcctgttcac cgtcgcgcaa tgtggtatcg aaaataatga
    16381 cttgctggct catggtttgg gtccttgtct ctttttgagc gcctcgcttc gggcataaaa
    16441 aaacccgcgc aatggcgcgg gttttttgtt tgactgagtg ctgacttaat tctgaatgcc
    16501 gctcaccagt ctaccgcgca aagaagatgc gtttagtagt agtagaccga taaagcgaac
    16561 gatgtgagtc attaaatcag ctccagatga atgcgatatg cttttagagt tactggatac
    16621 caaaacggat gtcaaccctg atgcaacaaa aacgtccagc cagcgtgagt tctgcatccg
    16681 tcaaattagc taattgtgct gcggtggtta aaacaagcga tattaatttc tgcttaacca
    16741 ccgccgcttt tcatcggttg acatatttca gaataaattt ttgcatctaa tcgacgaggg
    16801 aaagggggat aaaatgcacg cgttacaaa
//
LOCUS       DAZKGF010000108        16758 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZKGF010000108 DAZKGF010000000
VERSION     DAZKGF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.108"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            292..498
                     /locus_tag="ACHOQV_003297"
     CDS             292..498
                     /locus_tag="ACHOQV_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFP9631621.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            574..870
                     /locus_tag="ACHOQV_003298"
     CDS             574..870
                     /locus_tag="ACHOQV_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP9631622.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIQYMVETIAHHQVDIDSEV"
     gene            876..1661
                     /gene="bet"
                     /locus_tag="ACHOQV_003299"
     CDS             876..1661
                     /gene="bet"
                     /locus_tag="ACHOQV_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP9631623.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIHASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            1658..2338
                     /locus_tag="ACHOQV_003300"
     CDS             1658..2338
                     /locus_tag="ACHOQV_003300"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9631624.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            2335..2517
                     /locus_tag="ACHOQV_003301"
     CDS             2335..2517
                     /locus_tag="ACHOQV_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP9631625.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            2490..2681
                     /locus_tag="ACHOQV_003302"
     CDS             2490..2681
                     /locus_tag="ACHOQV_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP9631626.1"
                     /translation="MHKASPVELRTSIEVAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            2758..2973
                     /locus_tag="ACHOQV_003303"
     CDS             2758..2973
                     /locus_tag="ACHOQV_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9631627.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            3072..3281
                     /locus_tag="ACHOQV_003304"
     CDS             3072..3281
                     /locus_tag="ACHOQV_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9631628.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQ"
     gene            3338..3616
                     /locus_tag="ACHOQV_003305"
     CDS             3338..3616
                     /locus_tag="ACHOQV_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9631629.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            3588..3653
                     /locus_tag="ACHOQV_003306"
                     /pseudo
     CDS             3588..3653
                     /locus_tag="ACHOQV_003306"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3815..4978
                     /gene="intD"
                     /locus_tag="ACHOQV_003307"
     CDS             3815..4978
                     /gene="intD"
                     /locus_tag="ACHOQV_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFP9631630.1"
                     /translation="MSLFRRNEIWYASYSLPGEKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(4994..5070)
                     /locus_tag="ACHOQV_003308"
     tRNA            complement(4994..5070)
                     /locus_tag="ACHOQV_003308"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5034..5036),aa:Arg,seq:tct)
     gene            5341..6207
                     /gene="folD"
                     /locus_tag="ACHOQV_003309"
     CDS             5341..6207
                     /gene="folD"
                     /locus_tag="ACHOQV_003309"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFP9631631.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            6209..6421
                     /gene="ybcJ"
                     /locus_tag="ACHOQV_003310"
     CDS             6209..6421
                     /gene="ybcJ"
                     /locus_tag="ACHOQV_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFP9631632.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            6529..7050
                     /gene="ybcI"
                     /locus_tag="ACHOQV_003311"
     CDS             6529..7050
                     /gene="ybcI"
                     /locus_tag="ACHOQV_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9631633.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIVLAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHKVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(7086..8471)
                     /gene="cysS"
                     /locus_tag="ACHOQV_003312"
     CDS             complement(7086..8471)
                     /gene="cysS"
                     /locus_tag="ACHOQV_003312"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP9631634.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            8645..9139
                     /gene="ppiB"
                     /locus_tag="ACHOQV_003313"
     CDS             8645..9139
                     /gene="ppiB"
                     /locus_tag="ACHOQV_003313"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFP9631635.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKTTKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            9142..9864
                     /gene="lpxH"
                     /locus_tag="ACHOQV_003314"
     CDS             9142..9864
                     /gene="lpxH"
                     /locus_tag="ACHOQV_003314"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFP9631636.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            9982..10491
                     /gene="purE"
                     /locus_tag="ACHOQV_003315"
     CDS             9982..10491
                     /gene="purE"
                     /locus_tag="ACHOQV_003315"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP9631637.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            10488..11555
                     /gene="purK"
                     /locus_tag="ACHOQV_003316"
     CDS             10488..11555
                     /gene="purK"
                     /locus_tag="ACHOQV_003316"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP9631638.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPVQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(11888..12781)
                     /gene="ybcF"
                     /locus_tag="ACHOQV_003317"
     CDS             complement(11888..12781)
                     /gene="ybcF"
                     /locus_tag="ACHOQV_003317"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9631639.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTKDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(12778..13593)
                     /gene="ylbF"
                     /locus_tag="ACHOQV_003318"
     CDS             complement(12778..13593)
                     /gene="ylbF"
                     /locus_tag="ACHOQV_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP9631640.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVMAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFIHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKS"
     gene            complement(13604..14863)
                     /gene="ylbE"
                     /locus_tag="ACHOQV_003319"
     CDS             complement(13604..14863)
                     /gene="ylbE"
                     /locus_tag="ACHOQV_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP9631641.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLDQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPHIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIFLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(14873..16540)
                     /gene="fdrA"
                     /locus_tag="ACHOQV_003320"
     CDS             complement(14873..16540)
                     /gene="fdrA"
                     /locus_tag="ACHOQV_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP9631642.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSASSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVIPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQPHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLVRLLERLQ"
BASE COUNT         4080 a         4528 c         4388 g         3762 t
ORIGIN      
        1 ctatagtttt aaataagttc atcgcaacaa cacaacgata cggcaactac ctgattcacc
       61 gttgcgatga ccgcttagat ccgcagtttg aatttcagca ggcttcgggg agtgcgaggg
      121 gtgaaacgga cgcgtgaacg tcggtgtgac cagctgaaat taactcaaca tttcatacct
      181 tagtcgcttc aacgaggcgg cttagttatg acaaccggcg gccatccacc gcctgaatac
      241 gcgcagaagt ctctatatgt tcagcagccc agcttacggg caggagtttt tatggttcat
      301 caacattatg gaacgcagac cgttaatcga ggtgcggtca tgccaggaat gctggtcaaa
      361 cacaaagatg gtacctggac tgcatcagct aatttacgcg gacggcttta tctgcatcgc
      421 ggcatcgagc gcacttatac ccgtgatttg ctcgtggaag tttttctcga cggacgcggt
      481 aacggcctga atcactaatc ccctttcctg ttttcctaat cagcctggca tttcgcgggc
      541 gatattttca cagccatttt caggaggtca gccatgaacg cttattacat tcaggatcgt
      601 cttgaggctc agagctgggc gcgtcactac cagcagatcg cccgtgaaga gaaagaggca
      661 gaactcgcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc gctatgcatc
      721 gatcatttgc aacgccacgg ggccagcaaa aaagccatta cccgtgcgtt tgatgacgat
      781 gttgagtttc aggagcgcat ggcagaacac atccagtaca tggttgaaac cattgctcac
      841 caccaggttg atattgattc agaggtataa aacggatgag tacagcactc gcaacgctgg
      901 cagggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa ctgatcacca
      961 ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc gcattgctga
     1021 tcgtcgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc ttccctgata
     1081 agcagaacgg cattgttccg gtggtgggcg ttgatggctg gtcccgcatc atcaatgaaa
     1141 accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca tgccggattt
     1201 accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa tgccgccgcg
     1261 aaccattcaa aacccgcgaa ggcagagaaa tcacggggcc gtggcagtcg catcccaaac
     1321 ggatgttacg gcataaagcc atgattcagt gtgcccgtct ggccttcgga tttgctggta
     1381 tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact gcagaacgtc
     1441 agccagaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt aacactctgc
     1501 tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc cagatatttc
     1561 gccgcgacat tcacgcatcg tcagaactga cacaggccga agcagtgaaa gctcttggat
     1621 tcctgaaaca gaaagccact gagcagaagg tggcagcatg ataccggaca ttatcctgca
     1681 gcgtaccggg atcgacgtga gagctgtcga acagggggat gatgcatggc acaaattacg
     1741 gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaagcccc gctcaggaaa
     1801 gaagtggcct gacatgaaaa tgtcctactt ccacaccctg ctggctgagg tttgcaccgg
     1861 tgtggctccg gaagttaatg ctaaggcgct ggcctgggga aaacagtacg agaacgacgc
     1921 cagaaccctg tttgaattca cttccggcgt gaatattact gaatccccga tcatctatcg
     1981 cgacgaaagt atgcgcaccg cctgctcccc cgatggttta tgcagtgacg gcaacggcct
     2041 tgaactgaaa tgcccgttta cctcccggga tttcatgaaa ttccggctcg gtggtttcga
     2101 ggcaataaaa tcggcttaca tggcccaggt gcagtacagc atgtgggtga cgcgaaaaga
     2161 tgcctggtac tttgccaact atgacccgcg catgaagcgt gaaggcctgc attatgtcgt
     2221 gattgagcgg aatgaaaagt acatggcgag ttttgacgag atggtgccgg agttcatcga
     2281 aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat ggcgatgacg
     2341 catcctcacg ataatatccg ggtaggcgcg atcactttcg tctactccgt tacaaagcga
     2401 ggctgggtat ttcccggcct ttctgttatc agaaatccac tgaaagcaca gcggctggct
     2461 gagaagataa ataataaacg ggaggcggta tgcacaaagc atctcctgtt gagttaagaa
     2521 cgagtattga ggtggcacat agccttgctc aaattggagt caggtttgtg ccaataccag
     2581 tagaaacaga cgaagaattt catacgttag ccacatccct ttcacaaaag ctggaaatga
     2641 tggtggcgaa agcagaagca gatgagagag accaggtatg acaaccactg aatgcatttt
     2701 tctggcagcg ggcttcatat tctgtgtgct tatgcttgcc gacatggggc ttgttcaatg
     2761 acacctcagc aagaaaacgc ccttcgcagc attgcccgtc aggctaattc tgaaatcaaa
     2821 aaagccagac agcattttcc ggataaaaac gtcgatgaca tttgccgtag cgtactaaag
     2881 aagcaccgcg aaacggtaac gctgatggga ttcacaccga ctcatttaag cctggcgatc
     2941 ggcatgttga acggcgtctt taaggaacgg taaacatgaa aagcaaaatc atcagggagc
     3001 tacaggctcc ttttttatta ttcgcattta ccctcaagcg tattaaccaa caattcaggg
     3061 attaatgaaa gatggcagac atcattgatt cagcatcaga aatcgaagaa ttacagcgca
     3121 atacagcaat aaaaatgcgt cgtctgaact accagactgt atccgcaact cattgttgtg
     3181 agtgtggcga tccgatagat gagcgaagac gcctggctgt tcagggttgt cggacttgtg
     3241 cgagttgcca ggaggagatc gaacttaaga acaaacaata gggactgtga tggcctcaaa
     3301 gcagcaaatt tcaacatcgt ccaactgagg tgtaaaaatg ttcagaatca tttttcctaa
     3361 cacctggtac gtcgaccacc acggcactcc ctgcaaaatc ctgcgttcta cccacaacaa
     3421 agttcactac atccgaaaag gcagaacatg tatcgccagc atgttccgct ttaatcatga
     3481 ctttgaacct gtgaataaag ctgatgcaga tcggatagca gaagagatcg aaacggcaga
     3541 acacattaag aagttacgtg acatgcgttc aaaaagcaga ggtaaccatg gaatcataca
     3601 gcctcacact cgatgaggcc tgtcagtttc ttaagatatc cagaccaacc gccaccaact
     3661 ggatacgaac aggccgccta caggcaacac gtaaagatcc aaccaagcca aaatctcctt
     3721 acctcacaac acggcaagcc tgcattgcgg cgcttcagtc tccgctgcat actgtccagg
     3781 tgagcgcggg tgatggcata acagaggaaa gaaaatgtca ctcttccgca gaaatgaaat
     3841 atggtatgcc tcgtattcgc tcccgggcga gaaacgaatt aaggaatctc ttggcacaaa
     3901 ggacaagcgg caagctcagg agttgcacga caagcgaaaa gcagaactct ggcgagtaga
     3961 aaagctaggg gatttacctg atgtcacttt tgaagaggcc tgcctaagat ggcttgagga
     4021 aaaagctgat aaaaaatctc tcgattcaga taaaagccgg attgagttct ggcttgaaca
     4081 ttttgagggt ataaggctta aagatatctc ggaggcaaag atttactctg ctgtaagcag
     4141 aatgcataac agaaagacga aagaaatatg gaaacagaaa gttcaggccg ccatcaggaa
     4201 aggtaaagaa ccgcctgttt atgaaccaaa gccagtatca actcagacaa aggcaaagca
     4261 tcttgccatg ataaaggcca ttctccgtgc tgcagaacgc gactggaagt ggctggaaaa
     4321 agcgcctgtc atcaagatac cagcggtcag aaacaagcga gtcagatggc tggaaaagga
     4381 ggaagcaaaa cgccttattg atgagtgccc cgaaccactg aaatctgtcg tcaagtttgc
     4441 gctggcaact ggtctgagaa agtcgaacat cataaatctg gaatggcaac aaatcgacat
     4501 gcagcgacga gttgcctggg tgaatccaga agagagcaaa tcaaaccgcg ccattggtgt
     4561 ggcgctgaac gataccgcct gtaaagtgtt gcgtgatcaa ataggcaagc atcacaaatg
     4621 ggtgtttgta cataccaagg cggctaagcg agcagatgga acatcaacgc ctgcggtcag
     4681 gaagatgcgc atcgacagca agacatcatg gctatcagct tgtcgtcgtg caggaattga
     4741 agatttccgt ttccatgacc tcagacacac ctgggcaagc tggctgattc agtcaggcgt
     4801 cccattatca gtgcttcagg aaatgggcgg atgggagtcc atagaaatgg ttcgtaggta
     4861 tgctcacctt gcgcctaatc atttgacaga gcatgcgagg aaaatagacg acatttttgg
     4921 tgataatgtc ccaaatatgt cccactctgg aattatggag gatataaaga aggcgtaact
     4981 gattgaattg taatggcgcg ccctgcagga ttcgaacctg cggcccacga cttagaaggt
     5041 cgttgctcta tccaactgag ctaagggcgc attgatacca cggtgtgctg taatcgcgtg
     5101 aattatacgg tcaacccttg ctgagtcaat ggcttttgaa ccagttgctg aacaagtgta
     5161 cgaccgcatc agatgtagct cactatggcg gcaaaccaac gacggttatc atgataggtg
     5221 tgaatcagcc aggtacagaa ggttttactt ttcagaaaac gaaaactgac agcgcgccac
     5281 gcttctgaca aaatagacgc atccccttcg acctacgtaa cagatggaat cctctctctg
     5341 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
     5401 caaaaagttc aggcgcgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
     5461 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
     5521 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgctg
     5581 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
     5641 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta tacacccgga caaagacgtg
     5701 gacggtttcc atccttataa cgtcggtcgc ctgtgccagc gcgcgccgcg tctgcgtccc
     5761 tgcaccccgc gcggtattgt cacgctgctt gagcgttaca acattgatac cttcggcctc
     5821 aacgccgtgg tgattggcgc atcgaatatc gttggccgcc cgatgagcat ggaactgcta
     5881 ctggcaggtt gcaccaccac cgtgactcac cgtttcacta aaaatctgcg tcaccacgta
     5941 gaaaatgccg atctactgat cgttgccgtc ggcaagccgg gctttattcc tggtgactgg
     6001 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
     6061 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccg
     6121 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgctaca ggcgtgcgtt
     6181 gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta aacatccgca
     6241 cgttgagctg tgcgacttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     6301 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     6361 caaaatcgtc gccgggcaga cagtgagttt tgcaggtcac agcgtacagg ttgttgcctg
     6421 atccctttcc cggcctgtaa caacaggccg gttctctttt cagacttttg ccctctacgc
     6481 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     6541 attacgcacg ccgccgttcc cctttgcatt ggtttagggt tgggttcgaa agtcatccct
     6601 ccgcgtctgt tatttgccgg aatcgtcctg gcgatgctgc ccgatgccga cgtgttgtcg
     6661 tttaaatttg gcgttgctta cggcaatgtt tttggtcatc gcgggtttac ccattcgctg
     6721 gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg ggcgtcgatg gttcagggct
     6781 gggctgattc gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttgctggat
     6841 tcggtaacca ctggcggtaa aggggttggc tggctgtggc cgtggtcaga tgaacgcttt
     6901 ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta caccacgccg
     6961 tacgggcata aggtgattat ttctgaattg atgtgggtgt ggttgccggg gatgctactg
     7021 atgggaatgt tgtggtggcg cagacgataa ccggatgcga aaactcgcat ccggcaatag
     7081 cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc
     7141 tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc
     7201 agacgctgtt gaattaacgc ttcaatctca gccacttcac tgtcgtctgc ctgcgcgccg
     7261 ctttgcagga acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc
     7321 aggtgagacg ccattgcatt cgccgctgcc atatcttctg ctttcagacg gttcacttca
     7381 cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc gttcatcgcc
     7441 tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggag caacggtttt gtctgtgccg
     7501 cgcaaggcag tatagagacg ttccagcgcc gcacgcgcct gcttcaggtt ctcttcgctg
     7561 tagttcaact gactgcgata gtgacccgac atcaggaagt aacgcacagt ttccgcatcg
     7621 tagtatttca gcacatcacg cacggtgaag aagttaccca gcgatttaga catcttctcg
     7681 cggtcaacca tcaccatccc cgagtgcatc cagtagttca catactgacc atcatgggca
     7741 caggtggact gggcaatttc gttctcgtgg tgcgggaaca tcaggtcaga accgccgccg
     7801 tggatatcaa agtggttacc caactgtttg cagttcattg ccgaacactc aatgtgccag
     7861 ccaggacggc ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc
     7921 cacagaacga aatccatcgg gttgcgtttg tcgtcgacca cgtcaacgcg cgcgcctgcc
     7981 tgcagctgat cgagatcctg acgcgacagc acgccataag ttggatcggt cgggacgtcg
     8041 aacatcacgt cgccgttgtc cgccacataa gcgtgacctt tggcgatcag ttgttcagtg
     8101 agttcaataa tttcggcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg
     8161 ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag
     8221 ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcagt aatgttgcgc
     8281 acatacttca gcttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaggcaaca
     8341 aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacgtacatg
     8401 ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag
     8461 atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc
     8521 catatttccc gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg gttatgctat
     8581 aacaccaccc tatatatgac ccgaaccggg ttgaagcacc aatcaaacgg aacaggatgc
     8641 aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgacgataa
     8701 agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac
     8761 cattttccac cgtgtaatca acggctttat gattcagggc ggcggttttg aaccgggcat
     8821 gaagcaaaaa accaccaaag acccgatcaa aaacgaagct aacaacggtc tgaaaaatac
     8881 ccgtggtaca ctggcaatgg cccgtactca ggcaccgcac tctgcaactg cacagttctt
     8941 catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg
     9001 ttactgcgtg tttgctgaag tggttgaagg catggacgtg gtagacaaaa tcaaaggtgt
     9061 tgcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag
     9121 cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgcg
     9181 gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaagc ccgcaaggcc
     9241 gacgcgctgt atattcttgg cgatctgttt gaagcatgga ttggcgacga cgatcccaac
     9301 ccactccatc gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt
     9361 tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagcggc
     9421 atgacgttat tgccggaaga aaaggtgctc gaactttatg gtcgccgggt gttgattatg
     9481 catggcgaca cgctgtgcac cgatgacgcc ggttatcagg cttttcgcgc caaagtccac
     9541 aaaccctggc tgcaaacgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc
     9601 gcgcgaatgc gcgcgaacag caaagaagcc aacagcagta aatcgctggc gatcatggac
     9661 gttaaccaaa acgcggtggt cagtgcgatg gaaaaacatc aggtgcaatg gctgattcac
     9721 gggcataccc atcgcccggc agtacatgaa cttatcgcca atcagcaacc tgctttccgc
     9781 gtcgtgctgg gtgcctggca tactgaaggt tcgatggtga aagtcacggc ggatgacgtt
     9841 gagttgatcc attttccatt ttaaaaaacg cgcaactttg ctgatttcat aaccgcgcaa
     9901 ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc
     9961 accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg
    10021 atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg
    10081 aatgtcccac accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc
    10141 ttcgccgaaa gtgccgaaga gaacggttat caggtgatta ttgcgggcgc aggcggcgca
    10201 gcgcacctgc caggcatgat tgccgccaaa acgctggtgc cagtgctggg cgtgccagta
    10261 cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc
    10321 attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc gttactggca
    10381 gcacaaattc ttgcgactca tgataaagaa ctgcaccagc gtctgaatga ctggcgcaaa
    10441 gcccagaccg acgaagtgct ggaaaacccg gacccgcgag gtgcggcatg aaacaggttt
    10501 gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca
    10561 ttgctgtctg gccggtcggg ctggacgctg aaccggcggc ggtgcctgtt caacaaagtg
    10621 tgattaccgc tgagatcgaa cgctggccgg aaaccgcatt aacccgcgag ctggcgcgcc
    10681 atccggcctt tgtgaaccgc gatgtgttcc cgattattgc cgaccgtctg actcagaagc
    10741 agcttttcga taagctccac ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg
    10801 agtggcctgc ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt cgcactggtg
    10861 gctatgacgg tcgcggtcaa tggcgtttac gcgcaaatga aaccgaacag ttaccggcag
    10921 agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtgtcgctgg
    10981 ttggcgcgcg cggttttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc
    11041 aggacggtat tttgcgcacc agcgtcgctt tcccgcaggc caatgcgcag cagcaagcgc
    11101 aagccgaaga gatgctgtcg gcgattatgc aggagctggg ctatgtaggc gtgatggcaa
    11161 tggagtgttt tgtcaccccg caaggtctgc tgatcaacga actggctccg cgtgtgcata
    11221 acagcggtca ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg
    11281 cgattaccga tctgccgtta ccgcaaccgg tagtgaatag tccgtcggtg atgatcaacc
    11341 tgattggtag cgatgtgaat tatgactggc tgaaactgcc gctggtgcat ctgcactggt
    11401 acgacaaaga agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca
    11461 catcgcgtct gaccgcgacg ctggaagcct taatcccgct gctgccgcca gagtatgcca
    11521 gcggcgtgat gtgggcgcag agcaagttca gttaattgat gctcattttc ttgccggatg
    11581 cggcgtgaac gccttatccg gcccaccgct ccaacaccga ctgtaggcct gataagacgc
    11641 gccagcatcg catcaggcat ggtgtgccga ttgcaggatg cggcgtgaac gccttatccg
    11701 gcctaccgtt ccaacaccga ctgtaggcct gataagatgc gccagcatcg catcaggcat
    11761 ggtgtgccga ttgccggatg cggcgtgaac gccttatccg gcctaccgtt ccgacaccgc
    11821 ctgtagacct gataagacgc gccagcatcg catcaggcta gatgtgccaa ttgcctggtg
    11881 cccatcacta cagcgaaata caagtccccg cttcgcccgc cagcgtctct tcaatccgcg
    11941 ataacgcccc aatccacgcg ggtttgccac ggcttctgac atagccactc accgcagtca
    12001 ccttcggccc catcgaacca tcggctttgg caaatggcgc taactcatcc ggtgtggcat
    12061 ggcgaatggc acgttgctgc ggcgttcccc agttttcata taccgcatca gcatcggtga
    12121 ggatcaccag tccatcggca ttaatctgct cggcgagcaa cgcagcggcg agatctttat
    12181 caattaccgc ttcactccct gccccgtctt tcgtcacagg cacaccgccg ccgccactgc
    12241 aaatcaccac atgcccctct ttgagcaaca actcgatggc ttcgctgtcg agaattttac
    12301 gcggttgcgg agacgccacc acccggcgta aatatttacc atcacgtttc atctgccagc
    12361 cataagccgc ttccagtgcc tcttgttctt ctggctgata aaccggaccg ataaacttct
    12421 ctggctgcaa aaacgccgga tcatcaggcg aaacttcaat gcgcgtcagc actgtcgtga
    12481 caggcggcat ctgcggctgt gcgctcaagc tctgcgccag catatagcca atcatcccct
    12541 ggctttccgc caccagcaca tccagcggat acggttctac ctctttccac gccagattct
    12601 gtaacgccag cagccccacc tgcggcccgt tgccgtgaac aatcgccagc cgataagaac
    12661 gggccaggcg cgccagtgcg ggtacagcac tggcaatgtt gcgatattga ttttctgccg
    12721 tcagcgcctc accgcgctgg agtaaggcgt tgcccccaag agccacaacc agtgttttca
    12781 tgattttcct tgtaataatt gttggccaag ccagaacccc agcagcgtat ctgcccctga
    12841 cgtatgcccc agcgccagca gcgaatcaat cgcaacagcg gtacgtttcg ggcaactcag
    12901 agcatgaata aagtgcagga gtggcgaggc gaagtatcct tgcgcggcat aacgtaaata
    12961 actgacgctc accgctgtgg taacgagttg aagattgtcg gaacaggcaa aaaacgggcg
    13021 accggagcgc gcatctaaag cgccataata ccaggccgcc agcagcattc cgctcagcgt
    13081 gtcatcatga cttggcgtta atcctgggcc tttacccagc cagtgccgcc agtcggtctt
    13141 aacgccattg agcgcggcct gaaaacagtg acgaaactgg cgtaattcag caggaagcgg
    13201 atcgcctgcc gccaacgcca gtggcccgaa aagcccggtt tcctccgcgc gttgcatcca
    13261 tgccgctgca agcggttgag gatgcgcagg cggcgtaata cgcagcaaac aataacgctg
    13321 tggctgttta accgtgaaac gtccgaggcg aatcccttga gccataacct gtggtcgttc
    13381 attgccgcat aatccaccgc ataacgcatc gaattgcgcg cggcgaagca cccatcctcc
    13441 ggggccgaaa ccactaccct gacgatgcag cgttaacagt tcgccgcttt ccgtcatcag
    13501 gttaatcgcc cgccgccaca ctcccgctaa ccgccaggac tggcgataat tcggtgcgct
    13561 actactggca agcagaggat ggatgatcgt cattgtgcgt tcctcaacca atccccatgc
    13621 tttcagccag tgcttccagc gcctgctcaa agcacgccag cggtgcccgc acggtgcctg
    13681 cgccaatctg cccgatcccc gcctctttat gggcgattcc ggtattgatg agcggcgtaa
    13741 tgccggtttc taccacgcga cgaatatcca gccccaggca cgcgccctgg aaatcccagc
    13801 cggggatctg caactgcata ttgcgttcaa ggaagatttc cgccatctct tcagataccg
    13861 ctctggccgc ttccatgcca cccgcaccga caaagcgcgt tacgccaggc gctgcgatca
    13921 tcgccgcacc gccaatacca aaggtttcgg taattgcgct atcgcccata tccgggttcg
    13981 cctgctcctg cgagaagccg gtgaaaaaca gcccttgggg agtgtttaca ggcgcagtaa
    14041 accagcgttc gcccagcccg cttacccgaa tcccgaacat attgccgttg cgggtcatag
    14101 ccgtgacgat gctgcctgcg cggatcatcg caccagcatc catcgccgcc ttgcagtaag
    14161 ccatcgcgag gttaaggaag aactgatcgg tcacgctgag gaaatccatc acttccgcga
    14221 tgtgctgttt atcatgatcg aggcgagcaa tgtgtggggc cagcgcacgc attaacagtg
    14281 ctgaggaagc aatattgcgt tgatggaact catcgcccat ggtaatgccc tgcgccatca
    14341 tcgcggtgag atcgataccg cgctccatgc gccccagcgc cgcgcttaat actggcatta
    14401 acacatcgcg catccagcga tggcgagtca gaacatcttc gccgtaagcg ccaaaacgca
    14461 tcactttgcc gataccttcg ttgaggttgc agtacgcccg gttgccgtcg gtcacgttct
    14521 cgaccaccag catcggcata ctggcagaag taatgccgcc cattggcccg acggcattca
    14581 catggtgaca aggaatgaag ttcacttccc cctgatccag cattgccagc gcctgcgctt
    14641 catctttcgc ccagccttca aacagacatg cgcccacaca tgcccctttc atgggtccgg
    14701 tcatctcctg ccagcgcatt ggcggcccgg cgtgaagcag tgttttgccc tcgtttagtt
    14761 cgctgataag tgaagaagcc ggttgcacat ccagccagtg tggacgagcg cgacgaattt
    14821 gttcgataac cgcagcattg gcttgcgcca ctgatgtaaa catgggaacc ccttattgca
    14881 aacgttctaa taaacgaacc agttttttat tgccaccggc gactggcgac cattggtaat
    14941 gcacaaccgg tttgctggcg ctttgtagct ccagcgcaaa gctgcgtaat ccgatgttaa
    15001 tcacggcgac gttttccagt aatgacggtg tatgtggctg tgtggcaggc gagagcggat
    15061 gaattaacgc cgccgccagc aaggtggctt ccggcagcga actcacgacc gcaatccccg
    15121 catcttccag cgtggcgatt tgctgcgagc ggcattgcgg gtcacgttca gtgcctgtca
    15181 ccgtggcaat ggcgtacagc ggttggctgt cagaacgcgc ggcacaggct ttttgccagg
    15241 cgctcaccag cgaggcggca ggatcggcgg tcgcaccgaa gccaatcacg acatcaagca
    15301 gcaacacgcg cacttgcggt ttagcgccga gatcggcaat taacaggttg cgtaaggtcg
    15361 gatcgatcat cggatgggga cgcccgacgg tgtaaaaatc atcgcccagg tcgaggatct
    15421 gatgtccgtc ggcgtccagc atcatgccat gttgatgggt atcgtcggct tccacgccaa
    15481 ggtgtccggc aagtaatccc gccgcttcag cagccagcgt accgccggta tacaagccgc
    15541 aaataaatcc gctgctgaca ggcgctattg cgttacgtcg cgcagtgacg cgtgaaagca
    15601 gacaagccag gcgtgcggcc tcatccagcg aggaggcaaa ccagacattc tcgtcgcggg
    15661 ccaccgccgg ggtataacct aaaaacagcg ccaccgtcgg tttgccagtt gctttcatgg
    15721 cattaacaat tttcagacgc accgcttcgg caggtggttt tgaaacaaat gccagcactt
    15781 cgcttttctc gtctgcactg agcatttcca gcgccgtcag cgcactgatg ccgcccactt
    15841 cacggctgag gtcgcgcccg ccaaggccaa tcgcgtgagt aattccctcc cctgccagcg
    15901 caatctgtga gcacagctcc tgaatcccgg taccggaagc gccaatgacg ccaatgttgc
    15961 cttccggtat cacgttagca aaagccagcg gcgtgccggc aatcatcgac gtaccgcagt
    16021 ccggccccat caccagcaag cctttttccc gcgcgcgggt tttaagttgg atttcatctt
    16081 ccagcgtgac gttatcggag aacatcatca cgttgaggtt gcgatccagc gcctggttcg
    16141 ccagctccgc cgcatactca ccagccactg aaatcagcgc cagattggca tcgggtaatt
    16201 tctgacaggc actgtcccag cgacgcacct gcgtcaacgc ctggctgctg gctgacccct
    16261 gcgccagttg ttttagcgcc tcttcaagct gctgcataat cgcctgcgcg atccccgcat
    16321 ccgccgcttc gctacgaatt gccacgcaaa tatcgttcgg cgtggcgtta ttaaaatcgt
    16381 catgccagaa acctgtggta tctaataacg ctttattggc gggcgtaccc atcattacgg
    16441 aaacatcatc aacattttct gattcgctga gttttcgtga aataatcatt aaactgaccg
    16501 aatcctgaaa acaccctttt ttaataaagg cgtggatcat aacaactcct taaaaattgc
    16561 acccatggca attgcacgag cagtgttaat gtggcgatta cggtatcgat gacaacgagt
    16621 aaataaggtg atgaatatca cacagggatt attaacaaaa gaattcaaat aaccgccatg
    16681 ctattacgtg ttatttttta ataagcgtgg ttgttttaaa aaccaggtta aaagcgagcg
    16741 gattatcaaa taaattct
//
LOCUS       DAZKGF010000109        16685 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZKGF010000109 DAZKGF010000000
VERSION     DAZKGF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.109"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1172)
                     /locus_tag="ACHOQV_003321"
     CDS             complement(<1..1172)
                     /locus_tag="ACHOQV_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFP9631643.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDSSGTVVPAVAGGDIVWNYSKGSGE
                     GTLSQDGKVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTVRP"
     gene            complement(1543..2016)
                     /locus_tag="ACHOQV_003322"
     CDS             complement(1543..2016)
                     /locus_tag="ACHOQV_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631644.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRIILAREFFTPENRKSVPAHD
                     ESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYMGLGKNGLKYRSVKHLVGRA"
     gene            complement(2103..3347)
                     /locus_tag="ACHOQV_003323"
     CDS             complement(2103..3347)
                     /locus_tag="ACHOQV_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFP9631645.1"
                     /translation="MSKKFTKTLLSSSLVVGLLSVSSFTSASEYSYTALDIVGRHGTT
                     YTIVYNSDSDTAYVKWKTVPADAEGIESGSIVQGLSKINRDGTVEVTTKDGTSTYRVY
                     DREFHDLMNSLVKQKREGSLGKNEGTKTELVSDADIRNLFGRIAAISEMETNVGLDEY
                     GRVATSAESIKERVSLQSYLATEAANSAIGLDKNGKLTLNETDIVTPLRINDGVVTIA
                     KDVEEQKEGLAIQKKGLSELSSRTDIIDAATAANTNKIDSRTTVGVNADGTLTSADGA
                     TKTIAVNDGLVALSGRTDRIDATVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQ
                     IRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTA
                     AKAGVAASDGDVSYNVGVNFEF"
     gene            complement(3707..12094)
                     /locus_tag="ACHOQV_003324"
     CDS             complement(3707..12094)
                     /locus_tag="ACHOQV_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFP9631646.1"
                     /translation="MAFSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFSNPQNAIDH
                     STSFSLGDVLPTMGKGVAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPGV
                     ATVQDIFAGGSRGADEVIDTLPDGKNVVTDTVGKGLKATGKAVSDGAKASDEWLTEQM
                     SPGAVRALNTPITEGYDDSAVLLAKGVNLIGALVPDMVAGGVARKVGDVTLRKMLTAG
                     LEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLNA
                     DYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAVN
                     AMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGVINGAQNAYSRYQENMALRETAG
                     MDVSPWEGVTDAGIEGTVFGAAMGAPFGAVAGYRGRRQAAEEAAMRDAETVQQDDAAP
                     QPEPVDPVAQHRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQA
                     KRQAVMNELKAKPRSELLEEYRRLSQKEERTETEEQQFQAIREVIRPQQEVTPEAQSQ
                     PENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMGS
                     DVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPMEQ
                     PAFMRDPRFRGFTGDDTEVQARLARGNAPMAEELVRSQMAEGDAGPTAQELTERPRLP
                     APGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAPAPENAAGRQ
                     GETLEGEIVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPELA
                     QQAEVRRQAGGNRDIPQPETIAPESETTVFTDRESTVRGGEVRGKKIEDFGEEIKGAA
                     KHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDIP
                     AKTKHNTAGWGESVKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLPP
                     SQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKAKV
                     WFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASD
                     ARKYIDSHRDELVRHVKDMREISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRG
                     VQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAAH
                     YEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSNDGKITSGGDFMTEAQRVRRI
                     FKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAAR
                     AFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRDAFDHLFSTL
                     KTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAAEAKGSPVPQVEAVARGVMSGIKDS
                     DLKVRVVKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHE
                     IIHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLA
                     HMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYT
                     PREDGAGASSQYSRSGKPDPFKADAYDAKQFASEVRRIAGLQSVPRGGEVRVGDTPPV
                     LRALGAPNKELVMPAHVVHKATRPELREHYVSVETMADLPQLLADPVAVMTSKTEHDS
                     LVTLIEAKDRRGAPVIVAIHMRAKGAVAEVNKIASVYGKDNRIALQEQLDTSLLYLNK
                     EKAADWLLSAGLQLPGANTINGSSGFKVLHPEDIRKGAYYSRTSSLTPEETIASRFVR
                     QMQDKFQVLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKL
                     LANYKISQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGK
                     QAQYDRLAGIVDDMLARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLP
                     RTGKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNP
                     DKDYWQVFTDDRPDTMRTIAERKDQETGETRREVVERPVPMAMMADRYFTTKKNGKTY
                     YIKLHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTA
                     VMNLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWK
                     EFVEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVE
                     NALRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTAN
                     LVRTLGHLNGEGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWY
                     DKVPSHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRA
                     AGNVVGGVLGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENM
                     PFGTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDVTPESLKYWIDYISGG
                     TGRFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAE
                     LKSLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMV
                     KSVERDMKIAVDRFNREYNKKVGVE"
     gene            complement(12180..13442)
                     /locus_tag="ACHOQV_003325"
     CDS             complement(12180..13442)
                     /locus_tag="ACHOQV_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631647.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHRKENDQRTYDLNVRNADREYALKEREHKAAQNYRNASLGLSQQRLQLEKY
                     NQRRLEYNDMIAHSQPLMEALGKAIDAGDQEVVTRLFGQLPKGHPLILMSNEGYAAKA
                     GQAVNNLQKILDDKPDRAIDSLNTPETLDMLSGVFAPELQQRIGMPDSTGKKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLAKLQEVASNVAADAIKNGRDAQAEVDEFYAANGLQ
                     PHQQKVQQRKTQQLLQKWQTWAAGDPVKQEFVKEAAANAPALLEPGREKEVEIYYQNH
                     LRDKKAEHEKALDYSASAAAEKIKGWMK"
     gene            complement(13453..13704)
                     /locus_tag="ACHOQV_003326"
     CDS             complement(13453..13704)
                     /locus_tag="ACHOQV_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFP9631648.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTDIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGALF"
     gene            complement(13714..14160)
                     /locus_tag="ACHOQV_003327"
     CDS             complement(13714..14160)
                     /locus_tag="ACHOQV_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631649.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            complement(14163..14819)
                     /locus_tag="ACHOQV_003328"
     CDS             complement(14163..14819)
                     /locus_tag="ACHOQV_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631650.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYQKILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARRELAGNLAQSGVDPSSGRFNAVMDANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            complement(14913..15266)
                     /locus_tag="ACHOQV_003329"
     CDS             complement(14913..15266)
                     /locus_tag="ACHOQV_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631651.1"
                     /translation="MQRVVRNSRNPEFMNEVKDACLKKQAFCFEAPDGFLVLRSVLSA
                     DGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHTARRGFIRLGKRLGFER
                     MPDDEDGFMVFRIAV"
     gene            complement(15400..16626)
                     /locus_tag="ACHOQV_003330"
     CDS             complement(15400..16626)
                     /locus_tag="ACHOQV_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP9631652.1"
                     /translation="MKMTGKLLLAFVPALSLSVFSADAYAVDTSANSSGISGSPYNGI
                     TVDVLKSKQYADINSFWNDLGFPGKGYQDGSIVKVENGKITGPASVDIQNTAGKYLKY
                     SLYDAGKSGLTNTELLAPRSEIASYYDYANKVAYFVNTKTNQILFSADDKGLVTTVGT
                     DGKTYSRHVPEISEYIYEIKIKSVIPGSTINTVLFAEDVTDIHARLSALGSGETQVGG
                     GAITGGENATAVGYNATANYTNSVAVGANSQTTRPDEVNVGNRVIGGLRDGVQAADAA
                     TYGQLERYRIAEENARIQGDAQTLKSANTYTDNRVSTLERSTNQQFRQLRDEVEKNRK
                     RADAGIAGAMAMTAIPVVEGKTYSFGMAASNYRDEQAIAAGMHFRTTENSAVRLNASW
                     DTQNGSGVAAGMSVGW"
BASE COUNT         3757 a         4949 c         3621 g         4358 t
ORIGIN      
        1 ggtctgaccg tataatcacc attaaacgtg agcgaacccg ccccctggac aacatcatcc
       61 ttcagaacaa ccgtgccctt acccccaaag gtgatatctt tcccgtcatt cagactccct
      121 cccctgaagc cgttcatctt ccataccttc ccgtcctgag aaagggttcc ctctccgctt
      181 cccttactgt aattccacac aatgtcacca ccggcaactg ccggcacgac ggtgcctgaa
      241 ctgtcctgct ttatcgtgtt attcacatcc cctgcaggaa tcaccgtaaa ccagttcgtc
      301 ttcccgttct caccggcata accatgaagt acagcaacaa atacccactg cttcttcacc
      361 gcatcccagg caaaaagcgg tgagccactg tctcctgatg tcccgtaaga cgccagcgga
      421 ccattaacac cctggtaaag atttcccggg tttgacacca gactccagtc agaaatgacc
      481 ggacttccca ccgtccctcc agtcctccag acgtaagcat ccgctattct ggtcagcgca
      541 ccggaagcag ctccggtgta ctgcgttcca ctccccatac gataaaacac cggataacga
      601 ctgcggttct ggtatacccc cttgccggtt ccggcatccg tgacgctcac cggggcaact
      661 tctgtcacca gtttgttcag ccggggagta tgaaaatcac gccagtgctc attacggtca
      721 acaaggcgat atgtcgtatc gtccgcataa ccaaaacgca ccgtattgta gctgccgtta
      781 tgcttaaccc ccgccacata ctgcggagaa atcagtgttg ccacccccag tatctggtcc
      841 acaacactga aatccatcat cggagccttg tccagccgcc cactgacagc cccattctta
      901 ccataaagag gaatatttgt ggccccggca tgaaacgccc ctttgttttc agcaaaatcc
      961 ctgtaggtct gccatggcag agtattcccg acaacggatg ccagcgcagg agaagaaaac
     1021 agaacgccgg caacggctcc tgaggccaga agaagacggg aaagcatccc cgcaccggga
     1081 atgccggacg actttttcct gccccccacc gtttccggta cagcaaccag ttcttttctg
     1141 tcattaactc tgagtgagta tattttgttc atcccctgga ttccctgtat tttctcccgg
     1201 acaacaataa acagcgggca caatcatcat actaaaacga actttgactg ataaagataa
     1261 aatcaccatt aaccaattga tttaaataca taaacccaca ggcatttaac aagttctcag
     1321 ttatcagaaa gttcgaatta aggaagtaaa ctctcttccg ttttcacctc attatcagag
     1381 cggccagcac acagaatgtc accacacccc ccagccatac aaacagcatg aagcacatcg
     1441 cacccgctgt gtatttccgc tttccgcatc tgattttttt ctgttccatt ctcactgcca
     1501 ccgttccact ctcattaccc gactcacgaa tatgcattcc ggctacgcac gtccaaccag
     1561 gtgcttcaca ctcctgtact taagaccatt tttaccaagt cccatataca gaaaggtgtt
     1621 caccgtcttc gtgtttatcc ccatttcacg ggcaagatgg cagggctttg actcccggta
     1681 cacgtatcca aacatcacca ccatctcgga catcgtcaga ggtcgcgcat acgtattatg
     1741 tacccactca ccattccgga ttttcctgta cgtctccagg cgccccgtaa catcttccgc
     1801 atcaatctga agataacggc gtccctgaaa catgcagcac atcagcctgc caaggagggc
     1861 atcacagatt atcacccatg actcatcatg tgccggtaca ctcttcctgt tctctggtgt
     1921 aaaaaactcc ctcgccagta ttatcctgta cccctcaagc atctgctgca ggccatactc
     1981 cagataacgg ttgcgggtcg ccagaatcac tttcatcgct gtcacctgtt tcatgctcac
     2041 atgagaaaaa acggcaggca aaactccacg cctgccgtca tgcttcatgt cactgtcagc
     2101 aattaaaact caaagttcac acctacgttg taggacacat cgccatcact cgctgccaca
     2161 cctgctttcg ctgctgtctg ctcattgaaa cggtaaccaa cacccacggc aacagcctgt
     2221 ttatcactgt acccccccag ggcagcagtg gcgttgaatt tccccacact gtacggctgg
     2281 aaaagccccg ccagcgctgc actctgtgct gctgcacgct tcatctcttc gtgattctca
     2341 cgaatttgac gctgctggct gttcaggcgt gcattatgtt cctgcaactg gcgagtattg
     2401 gactgaatag cctgtgtatt tttagtaacc cggcggtcaa cgctaccaac agtagcatca
     2461 atacggtctg ttctgccgga cagagcaacg agaccgtcat taacagcaat cgtttttgta
     2521 gcgccatcag cgctggtcaa ggttccgtcc gcattcacac caacggtggt tcgggaatca
     2581 attttattcg tattcgcagc cgttgctgca tcaatgatat ctgtgcggga tgaaagctca
     2641 gacagtcctt tcttctggat cgccagccct tctttctgtt cttccacatc tttcgctata
     2701 gtgacaacgc cgtcattgat acgtaacggc gttacaatat ctgtctcatt cagagttagc
     2761 ttaccgtttt tatctaggcc aattgcgcta tttgcagcct ctgtagccag ataactctgc
     2821 aaagacacgc gttctttaat actctctgca ctagttgcca ctcgcccata ttcatccaga
     2881 ccaacattag tttccatttc agagatcgca gcaattctgc caaaaagatt ccgaatatca
     2941 gcatcagata caagctctgt tttagtaccc tcgttctttc caagagaacc ttctcttttc
     3001 tgcttgacca gtgaattcat taggtcatga aactcccggt catacacacg atatgtacta
     3061 gtgccatcct tggtagtaac ttctacagtc ccatctctat taatttttga taatccttgt
     3121 acaatacttc cactttcaat cccttcagca tcggcaggta ctgttttcca tttaacataa
     3181 gcggtgtcag aatcactatt ataaacaatg gtgtaagtag ttccgtgtct accgacaata
     3241 tccaaggcag tgtagctata ttcacttgca cttgtaaaag aactaacact caacagacct
     3301 acaaccagag aagatgacag aagtgtcttt gtaaattttt tactcattat ggatatcctg
     3361 ttaaattatc tgtatacaaa tcagccacta cacctcatac tcataaataa atatgattaa
     3421 ttgcggcttt attcaggata ttccgaaaat aaccccaaaa tattaattta atgttttagg
     3481 atttgtctta cattattctt ggtgtagtca ctcagaacca cgactacacc accggaacag
     3541 gcatcagaaa aaacttactc tcatcaggca ttctgtttca catctgctta cactgtaatt
     3601 tctgatcaat atcacaacat tatccgaatt taactgttca gttcatctac aatatttccc
     3661 tgcgtgactt tatatcagcc ccgcacttcc gtacggagca tgttctttac tccactccca
     3721 cttttttgtt gtactcgcgg ttaaaccgat ccacggcaat tttcatatcc cgctctaccg
     3781 atttcaccat cgccgactgt tgcgccagac tgagagtact gtcggcgtaa atggcatcac
     3841 gctgtttacg caaatccttc agtctcttcc gggtatcctg cataagcccg ttcatcgaca
     3901 attttccgtt gttctcgtca atgaacgccg ttctttctgc accggtcaga ctcttcagct
     3961 ctgcgtggta ctgcgcaacc tctgtcatcc ggtcgtacat cttctgctgg tcagcatacg
     4021 gcatcacctc acctgaaatt ttccccagga aaggcacctg ctgctccggt atatcaatac
     4081 cattcagcga tttcaccgcc gcatccgtgg ttttggaaat gaagcgccct gtaccaccag
     4141 agatatagtc aatccagtat ttcagtgatt ccggcgtgac atccaccgcg ccgggacggt
     4201 actggctgcc acctgagaac gcattcagcc aggatgcaaa cgccttgtac gcttctggcg
     4261 ttgaacgtct tcccagctgg ctgtcaggtt ttggtgtacc aaacggcatg ttctcctggt
     4321 aaatctgcgc ccccatgaag ttttcattca tggcaaggtt cgcaaacgga cgcagaatgg
     4381 tcggcgctgc atttttcagc aatgccccgg acagtgtttc cgacgtctca ctgccaatcg
     4441 ggctgaatgc accaagcaca ccaccaacaa cattaccggc agcacgggac gccgtcaggt
     4501 cacccgccgc cacaccttca gcggtatgcc cgagcaggaa gaaaacgttg tacccgtaag
     4561 gcagaggaat actccagtac tctccggcct tgcccccgaa caccgatttc ataatgacga
     4621 ggttacgctc tttcacatga gacggcacct tgtcatacca gttaaccccg tcatcatcct
     4681 cacccgccac actgcggtta agcgagccaa gcagataacc tgctcccaca gcggcaagcg
     4741 cgattttctg cggaacattc agattcttcc agcgaaggcg ctccagtaaa ggtccctcac
     4801 cattaagatg tccgagcgtt ctcaccagat ttgccgttcc ctgaatactg gcgttggcga
     4861 acatgtacag cgagttcatc agcgccccct gctcaccacg acggttaaag ttcaccgtca
     4921 tgtttttggc aagagacgcc gcctgctggc gtgacaaacc ggcatcacgg gcgtgtttat
     4981 aagctgacag acgcagtgcg ttttcaaccg cgccgttggc atcctcgaca aggttaagga
     5041 acgaattcca tgcaccgata ctctggcctt tccatcctcc cttcgccagc gaaacaaggc
     5101 gatccatttc cttctgctgg ccttcaaggt cacccatgtt aaaccaaccg gttttacctc
     5161 cgtcctcaac aaactctttc cacaccttct gccactgtgc acctttgccc gtgagggttt
     5221 taccacgcag actggcgtat acggctgaca tggcagaacg gctgtctttg actacagcca
     5281 gggcggataa gttatccagc cctttcagtt taccgtcgct ccttccctgc tcagccttca
     5341 ggttcatcac cgccgtctgc acgtcacgga tgaagttact gaccaggaat tccgggttat
     5401 acgacgtgtt caccgttgcc aggaagcggt taactttccc cagcgtacgg attacggcgt
     5461 tactggtttc cggtcccata ttcttcatcg cacgcatcag gcgcgggtca tggagtttga
     5521 tgtagtacgt tttgccgttc tttttggtgg tgaagtagcg gtctgccatc attgccatcg
     5581 gtacagggcg ttcgacaact tcgcgacggg tttcaccagt ttcctggtcc ttgcgctctg
     5641 caatcgtccg catggtatcc ggtctgtcat cggtgaatac ctgccagtaa tccttgtcgg
     5701 gattatcctg caccagtttc aggaaggcgt tacccacttc gtttttgcga tggcggatca
     5761 gcgattcgct caggtcctgt atcgcctgag tggacggaga ctgtgcacgg gatgcacgcc
     5821 ccatggcctg cctgctttca cgtccgccga tggtgaagcc cttacctgta cggggaagtg
     5881 acaccacacc gtcaacatcc tgacctttca ggggaacgta gtaacggtag gcgttctgcc
     5941 aggcatccac cacaccgttc tcttcaagtc cggcctcacg gataagctca cggcgacggg
     6001 ccagcatatc gtcaacaatc cctgccagac ggtcatactg tgcctgtttg ccgctgttac
     6061 gcacacgctg catgatttcc gccgcctccg cgttggtcat ccccgaaccg ccgtccggca
     6121 ttttagggtt gattttcgca atatgtgcgt tacgttccgg cgcgtgacgg gcgtagaggt
     6181 actcatccag atcggcctga gagattttgt agttcgccag caatttggcc agcggctgaa
     6241 cgtagcgctc cttcattacg ttcaggtcgt tttccgcctt cccgtggaag agttcttcag
     6301 ccatataggc gttattgctg tcatctattt ttccgccagt tttacggata ttctcctgca
     6361 ccgccttcag cacctggaat ttatcctgca tctggcgaac aaaacgcgat gcaatggtct
     6421 cttccggtgt cagactgctg gtgcgggagt aatacgcccc cttgcgaata tcttcaggat
     6481 gtagtacttt aaatccagaa gagccgttga tggtgttcgc cccgggcaat tggagcccgg
     6541 ctgaaagaag ccagtcagcg gctttttctt tattcagata cagcaaggaa gtatccaact
     6601 gttcctgcaa agcgatcctg ttgtcctttc cgtagacact ggcaatctta ttaacctcag
     6661 ctaccgcccc tttagcacgc atgtgaattg caacaatgac aggtgcacct cttctgtcct
     6721 tagcctcaat caaagttacc agtgagtcat gttccgtctt tgatgtcatc accgcaaccg
     6781 ggtcggcaag caattgcggt aagtcagcca tcgtttcaac agaaacatag tgctcacgta
     6841 attctggtct ggtggccttg tgaaccacat gagctggcat aaccagctct ttatttggtg
     6901 cgccaagtgc tcttaacact ggcggagtgt caccaacccg cacctctcca cctcgtggaa
     6961 ccgattgtaa tccggcaatg cgacgcactt ctgaagcaaa ctgctttgca tcataggcgt
     7021 cagccttaaa cggatccggc ttaccactac gggagtactg agacgatgcg cccgcgccat
     7081 cctcacgcgg cgtgtaacct tcccgcacac gctggcctaa cgtacggatg gtctcgcgaa
     7141 caagtctgat atcgttcagt tccgtcggct tcagtaaccc cgtacgacgc agtacccctt
     7201 tgaccagggc aacaacacgc tcccatgccg ccacgaattt attcggctgt ttctccgcca
     7261 tatgtgccag aaattcaccc gcctgtactt ccggtgattc cttaccatag gacgcatcaa
     7321 ccttacgcca ggcttcacgg atggtggcgt tatcactgtc gcgggttttc agcacggttt
     7381 tgataatcgt ctgatattcc gctggcgtga caacatgctc catggcatgg tggataatct
     7441 cgtgacgcag cttctcgcgt acggtccgcc cgtcagggat gttatccgcc accaggacta
     7501 tttctcgttt atccggacga tagaatgcgt gcaccctgcc gtaaccatcg aacgattcgc
     7561 ccgccagcgc ttcagcctct ttctgtgact tcaccacacg gaccttcagg tcactgtcct
     7621 taatgccgct catcacgcca cgggcaaccg cttcaacctg cgggaccggg ctgcctttgg
     7681 cttccgccgc actacggtta acatccgaaa tgagattgcc ttcaggtgtg cgggttacgc
     7741 ccttacggga ataaaacgca acgcccttgt ccgtctcacg ggttttcagc gtactgaaca
     7801 ggtgatcgaa tgcatcacga ataccgccat ccagttccgc attcgtcgga taagcccagg
     7861 tgttatctgt gttgtgctca ggtgccttac ggatattgac cagataatca ttctccacgc
     7921 cagccatacg cgccttatcc tgaacataac gctcaaaggc acgcgccgcc atttcaacat
     7981 ccgttgacca gtacggtttt gagcgcacct catcaagaag cgctgaacga cgcggcatgt
     8041 cactgttttt aatggcctga atcacacctt taaaagcgtc gtaaacctcc tgacgtaccg
     8101 gatattcagc atcaacatac ctgccgtctt taaatatgcg cctgacacgc tgtgcttccg
     8161 tcataaagtc gccacctgac gtaattttcc cgtcattgga aacgtcataa cgaccaaaat
     8221 aattatccag agaatggaac cattcgtgcg ccagcgcacc cggtccgtta ccttttgtca
     8281 ggttgattgc cacctcaccc ggctcatagt gtgccgccgc cttaccctta ccacgggcac
     8341 caaatgccag gccaagacga ccgttcaggg aaagcgcttt tgtcggtaca ttcagtactt
     8401 ccgcaaggtc atgcagcgag tcataagccc ggttcaaatc agcctgacga cgcggacctt
     8461 ccacgtagtt accaaactgc acaccacgga aaccaaacgc atcactgaac tgctccggtg
     8521 aaacatcccc tttacggcgt tctggtccgg tacggtcgcg gttggtggcg ttacgctgct
     8581 cctcacgcga aatctcccgc atatccttca catgacgaac aagctcatca cgatgtgaat
     8641 caatgtactt acgcgcatca ctggctgact taaagccacc tctcacccgc attttgtttt
     8701 taccgtaagc gataaaaata tcgccactgc gggtattccg gtaaacgtca aagcggattt
     8761 tgtcatccgg tgacggtgct gttttttcat cacctttcgc ctgtgatttt tcctcctgct
     8821 ctgcaaacca gactttcgcc tttgccagta attcatccct gctttccgag aaaaagaggt
     8881 tactcccctt attgtcctta ttgcgcagtg aataaagttt ctgtggcgga tcgtaacgct
     8941 tccctcctgc cgcctgatac acacccgata ccacccgata agcagaagcc ctgtccatct
     9001 gtgagggtgg cagagtgcgt aacagttgcc aggtatccgc gtaacgggag ggcattctgc
     9061 cttccatcca ttctgcgagg cgtttcgcgc tgaccgttcc gttcagcatt tccgatacac
     9121 tgtgtcgtac tttttttacg ctctcccccc agcctgccgt attgtgtttc gtcttcgccg
     9181 gaatatcgct acgatacagc gctatcattg ccagagtgtc agcatcggca ccttcgttcg
     9241 ccagttttgc gtagtccggt ttcgggaaca gtttgctcag cggctgcgtg gcataatccc
     9301 tgtcttccag cgttttaccc agtgtttcag caagctgtgc ataacggtgt ttggctgccc
     9361 ctttaatttc ctcgccaaag tcttcaattt ttttgcccct gacttcacct ccgcgcacgg
     9421 tagattccct gtcagtaaag acagttgttt cagattcagg tgcgattgtc tcaggctgcg
     9481 ggatgtcacg atttcctccg gcctgtcggc gtacttctgc ctgctgtgcg agctcaggca
     9541 gtgagtcccg tacagtgcgg ggaagtgatt caggctgagg catacgaaca ttacgcgcaa
     9601 tgtcaggagc cggtagccct tcacgtaccg gtgctgtcgc gttctgcgca tccggtgacg
     9661 gcagaccacg acgaactatt tcaccctcga gtgtttcccc ctggcgacca gcggcgtttt
     9721 ccggtgccgg agcacgtcct ttctggaaac tctggccctt aacctcaccg gttgtggtaa
     9781 aacgaccacc acgtccggcc tgattctcat ccggcgtacg cgccacctct cccggtaacg
     9841 gatatccctg tccgggatga atatcgccgg gggcgggcag acgcggacgc tcagttaact
     9901 cctgtgctgt cggaccggca tcaccctcag ccatctgtga acgtacaagc tcctccgcca
     9961 tcggcgcgtt gccacgggca aggcgggcct gtacctccgt atcatccccc gtgaaaccac
    10021 ggaatcgcgg gtcacgcatg aacgcgggct gctccatcgg gtcagcatca gcaatacgct
    10081 ctccgtcagc cagggtgtta atggtttccg ttgccacatc ttcagagaac gcaggattat
    10141 ccatttcaac ggcatactga ttctcgcctt tcttcactac ggacggtttc aggccggtag
    10201 cggcggcatt acggaaaaca tcactcccca tggcgctttt ctcatccgtg aaataacggt
    10261 tgtccgggcg caccttttca atgcgttccg ctggtctgga cgcaccattc ccgttaattt
    10321 caatgcggac ttcattcggg tcccggaacc gcaccgtcgg gtaaatgctc ccgttaccat
    10381 cctccgcatt ttcaggctgt gactgtgctt ccggcgtcac ttcctgttgt gggcgaatga
    10441 cttctcgtat tgcctgaaac tgttgttctt cagtctcggt gcgctcctct ttctggctga
    10501 gtctgcggta ttcctcaagc agttcagaac gcggcttcgc cttcagctca ttcatcacag
    10561 cctgtcgctt cgcctgttcg tccagttcat tcaacaactg gctggcagct tcccggcgat
    10621 gagctgcgga tgcgtcaccc tctgttgcca tatccgcatc agcatactgt tccagaagct
    10681 gctcgcgatt catgccctgc atggattcac gatgctgtgc caccggatca accggttcgg
    10741 gttgcggggc tgcgtcgtcc tgctgcacgg tttcagcatc acgcatggct gcttcttctg
    10801 cggcctgacg tctgccacga tatccggcaa ccgcaccgaa tggagccccc atcgcagcac
    10861 cgaaaacagt gccttcaatc cctgcgtcag tcaccccctc ccatggcgat acatccattc
    10921 cggcggtctc acgcaatgcc atgttttcct gatagcgcga ataggcgttc tgtgccccgt
    10981 taattacgcc ctgttccgtg gcatttctga caatgccgct tttaacggtc ttcgctgtgc
    11041 ctcgtgtcac cagattaaac agttgtgcgt cacccagttt tgccgccatg gcattcacag
    11101 ccagcaattc aggatcggtt gccagctgcg cgcgcacctc atcggcaaca cgctcttttg
    11161 ccagatccat tttctggcga tcagtaagct gtgcatgctg cgggtcggcg tcaatggaca
    11221 aaaacgtctg ctgaaatttc ggtgactgcg ccagctcaga gtaatccgca ttaagaacag
    11281 catctgctgc cgccattgca ctctgcccct gtgcactggc tgtggaatgg gtgatcaggc
    11341 ccgcctggaa taaatccggc atttttttat cgacagcttc tgctgccagt gccgtggctc
    11401 tttccggctg catccctgcc gcgatgtatt ttttctccag cccggcggtc agcatttttc
    11461 gcagtgtgac atcacccacc tttctagcca caccgccagc aaccatatca ggtacaagcg
    11521 caccaatcag gtttacaccc ttcgccagca agaccgcaga atcatcatag ccttctgtta
    11581 tcggcgtatt cagcgcacga acagcacctg gcgacatctg ttctgtcagc cattcatccg
    11641 aggctttggc accatcactg acagccttac cggttgcttt cagaccttta ccgacagtat
    11701 cagtgaccac gttttttcca tcaggcaggg tatcgatgac ctcatcagcc cccctgctgc
    11761 caccggcaaa aatatcctga acagttgcga cacccggtag ccccatacgg ctgaactcat
    11821 ttaaaatacg cgcccctgtt tttaccgggc tctgaatcat cgcatcaccg agtccacggg
    11881 ccatttcccc tgttccccgg acggactggg caacaccttt acccattgtt ggcaatacat
    11941 caccgaggct gaacgatgtg ctgtgatcaa tggcattctg cgggttgctg aaaaaatcct
    12001 gccagtaatt ttctcccggc tgcttaatgt tcaggctgct gcggttctgg ttacccaatt
    12061 gcgcttcagg gcgctgctca tctgaaaaag ccatacagac tccagtaaaa aaaagcccga
    12121 cacgatggtc gggcgtaagt gaacgatatt aacggggagc gcgttactgc cgcatatctt
    12181 tacttcatcc agcctttgat cttctcggca gctgcggaag cgctataatc cagcgccttt
    12241 tcatgctctg cctttttatc ccggaggtga ttctggtaat aaatttccac ctccttttca
    12301 cgaccaggtt ccagcaaagc aggcgcattg gcggcagcct cttttacaaa ctcctgttta
    12361 accggatcgc ccgccgccca cgtctgccac ttctgtaaaa gctgttgcgt tttccgttgc
    12421 tgaactttct gttgatgcgg ctgtaaacca ttcgcagcat aaaattcatc cacctcagcc
    12481 tgagcatcac ggccattctt gatcgcatcc gccgctacgt tgctggctac ttcctgaagt
    12541 ttcgccagag aaagtccctg ctgcttcggc ataaaataac cataattttt cgatatctct
    12601 gcaaatttgc tgcgatccct gacctgagcg atagccttat caacgggtat cgccagcacg
    12661 gtttgatcat cagggtgcgc actgccgtat tctgttacag gtttatgcgc ggtggagcca
    12721 tcgctgtatg tgagatcaag gccaataagt acgtaccctt cctgctgcgc cggtacgata
    12781 ctgccaatcc tggcctcttt tatcgttttt ttcccggttg aatcaggcat accaatacgc
    12841 tgttgtagtt ccggggcaaa cacgccggaa agcatatcga gagtttccgg ggtattcagc
    12901 gaatcgatcg ccctgtccgg cttatcatca aggattttct gcaggttgtt cacggcctga
    12961 cccgctttcg cagcatagcc ttcgtttgac ataagaatta atggatgtcc ctttggcagc
    13021 tgtccgaaca gacgcgttac aacctcctga tcgccagcat caattgcttt tccaagcgct
    13081 tccattagtg gctggctatg ggcaatcata tcgttatact caagccgtcg ctggttgtat
    13141 ttttccagtt gcagtctctg ctgagatagc ccaagtgacg catttcgata attctgcgct
    13201 gctttgtgtt ccctctcttt cagcgcatat tcacgatcgg cattcctgac attaagatca
    13261 tatgtacgct gatcattttc ttttcggtgc tcaaactgag actgcgcaaa ttcaaaatcg
    13321 cgctcgttat ttttctgttg ctgagcaagc tgtgcttctc gcaaaccaag ctccttacga
    13381 cgggtcattg cctggtcaac agtgctgaat ccggcaagta acccctgtgc aaatccgctc
    13441 attcaccact ccttaaaaca aagcgcctgc aatgccgcca attactgcac cagcaacagc
    13501 gccaacagga ccaccaacgg atgcgccaat agccgccccc gtaccaatgc ccgtaccgat
    13561 attctgtttt ttctgagctt tctgttgagc agccagttgt ttatttgatg cctcaatatc
    13621 tgtacgtcgt ctgtctgcgt cacttattcc ctgtaatgcc tcacgccgcg actgatttgc
    13681 aatatccagt aaaccgtatc ccatattgcc cccttacgct gccaccagtt gtccgccaac
    13741 actcagtttc tgtcttgccg gtgcagaagc ccccgtcaga atattcatct ggcgatcctg
    13801 ttcggcttca cggataccat ttttcgcgcc agcaattgcc agggcattac gtaaccccag
    13861 cgtattactg tcgggattat ccgggcggtt taccccgtat ctcgccatct ggttatcctg
    13921 cgccatctgc gctgtacgga gactggaagt ggcaaggcct cccacccgtg caagctgtgc
    13981 attcatcaga ctgttgttct ccccaaggtc agccagcctt gctacacggg gtaaatatct
    14041 ggtgcgccag tcgtcgtatt gctggcgggt cagcgctgcg gatgtctgcc agtccccctg
    14101 agggcgggct gctccggtat aaccgtatct tgcaagtgtt tcgtatttac cgtactccat
    14161 aatcacagtc tccagttctg agcctgatgc tgaatggcat tagcgccggt gccaggtgtt
    14221 ttagcaccgc cacttcctcc gctaccgcca gccttatgca tcgcatatgc acctgccgca
    14281 cccagaccag cgccaacaag agaggctcgc ccctgctgtt tcgtaaacgc cgcctgtgca
    14341 tccgatttag cttttgccag actgctgtct gccagagagt taaaactctg taacgcatcc
    14401 gccttctgac cagaaccgag agcagcaaca tcctgtagcc cggcaacata cttatctgcc
    14461 tgcgacacct gcccccgtgt ggttgtgtca atctgcccgg tcacctggtc gctttgattt
    14521 gcatccatta ccgcattaaa acgaccactg gatgggtcaa cgccggactg agcaagatta
    14581 cccgccagct cccttcgcgc ttcaccaaac tgtttctgat aaccaagatt tgttgtgcca
    14641 gcaatattgt cgtactgctg ctcactgtta aggtcatcga ccttttccat gaagttatct
    14701 tctgccggac ggaggatttt ttgataatcc tgccaccctt tccaggccac ttcttcctgt
    14761 gctatttgcg ctgctgttgg ttttactttg gtatcaccac cgcctttact tccacccata
    14821 atggccccct ggataacaaa aaaccctgcc ggagcagggt cagaatgtga attacaatgt
    14881 tggtttaaac acaatgatga aaactgtagc agtcagaccg ctatcctgaa caccatgaag
    14941 ccatcctcat catccggcat tcgctcaaag cccagacgtt ttcccagccg gataaatccc
    15001 cgccttgccg tatgaaattc agcccagcgt ccgccagcca gacgggttaa tgtcttcacc
    15061 tccggcagat aacgctcaac gctgttactc cccgtacaca cgcccagcaa caccagaaca
    15121 taagggatac catcagcact gagcacagaa cgcagcacca gaaagccatc aggtgcctca
    15181 aaacaaaacg cctgcttttt aaggcaggcg tctttaactt cattcataaa ttcagggttg
    15241 cgggaatttc tcacaacacg ctgcatatac cggagaattt tttcactcac actctcacca
    15301 caaaaaagaa caatgcggct atactgtctt tagctacaga aggatgacgg agaacgcttc
    15361 cgacgaccaa cacggctggc gttctccgca aaaaataaat taccagccga cagacatacc
    15421 cgcagcaacc cctgaaccgt tctgggtatc ccatgaagca ttcagacgta cagcagaatt
    15481 ttcagtggtg cggaagtgca tacctgctgc gatagcctgc tcatcacggt agttacttgc
    15541 agccatacca aaggagtacg ttttaccctc aacaacagga atggcagtca tcgccattgc
    15601 tcctgcaata cctgcatctg cacgtttacg gtttttctcc acctcatcac gtaactgtcg
    15661 gaattgctga ttagtgctac gctcaagggt actgacccgg ttatctgtgt aggtattagc
    15721 agacttcagt gtctgtgcat caccctgaat acgggcattc tcttctgcaa tacgatagcg
    15781 ctctaactga ccataggttg cggcatccgc agcctgaaca ccatcacgaa gcccaccaat
    15841 tacacgattc cccacattaa cttcatcggg gcgagtggtc tgagagttag cccctacagc
    15901 aacactgttc gtatagtttg cagtggcgtt gtaacctaca gcggttgcat tctctccacc
    15961 tgtaattgcc cctccaccaa cctgagtttc tccagaaccc agtgcagaca gacgagcgtg
    16021 gatatccgtt acatcttcag caaaaagtac ggtattaatg gtgctccctg gtattacaga
    16081 cttaatttta atctcataaa tatattcact aatttcagga acatgtcttg aatatgtttt
    16141 cccatcagta cctactgtcg tgacaagccc cttatcatct gcactaaaca gaatttgatt
    16201 tgtttttgta ttgacaaaat aagcgacctt gtttgcatag tcgtaataag atgctatttc
    16261 actacgtggt gctaataact ctgtgttagt caaaccagat ttaccggcat cgtaaagaga
    16321 atatttcaaa tatttaccag ccgtattttg tatatccaca ctggcaggtc ctgttatttt
    16381 accattctca acctttacta tactgccatc ctggtaacct ttccctggaa atccgaggtc
    16441 attccagaac gaatttatat ctgcatattg tttacttttc agaacatcaa cagttatccc
    16501 attataaggc gaaccactaa tgcctgatga gttcgcagat gtatctactg catatgcgtc
    16561 tgcagaaaat accgacaggc ttaatgccgg aacgaaagct aacaatagct tacctgtcat
    16621 tttcatgatg tttatcccgt gttaattatg tcataaatgc atcgacatcc gggaacacgg
    16681 atgtc
//
LOCUS       DAZKGF010000110        16674 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZKGF010000110 DAZKGF010000000
VERSION     DAZKGF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.110"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..821
                     /locus_tag="ACHOQV_003331"
     CDS             <1..821
                     /locus_tag="ACHOQV_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9631653.1"
                     /translation="TVAVLVPSLTDKASSRFMQSLQQVLNKNEFQLLLGCHEYNQLKE
                     AEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPFSPAQITIETAFFE
                     ASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQNTDLVITMPDAAS
                     LQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPGNIAVACLDGSDNC
                     DQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFNYQIELRQST"
     gene            1218..1487
                     /locus_tag="ACHOQV_003332"
                     /pseudo
     CDS             1218..1487
                     /locus_tag="ACHOQV_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1661..2890)
                     /locus_tag="ACHOQV_003333"
     CDS             complement(1661..2890)
                     /locus_tag="ACHOQV_003333"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFP9631654.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARSATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(2887..3618)
                     /locus_tag="ACHOQV_003334"
     CDS             complement(2887..3618)
                     /locus_tag="ACHOQV_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFP9631655.1"
                     /translation="MSRSVLVTGASKGIGRAIACKLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(3618..4082)
                     /locus_tag="ACHOQV_003335"
     CDS             complement(3618..4082)
                     /locus_tag="ACHOQV_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFP9631656.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(4079..5248)
                     /locus_tag="ACHOQV_003336"
     CDS             complement(4079..5248)
                     /locus_tag="ACHOQV_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFP9631657.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTNGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAMISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDIG
                     YINLHGTATQLNDQIESMVVNALFGKRIPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(5250..5834)
                     /locus_tag="ACHOQV_003337"
     CDS             complement(5250..5834)
                     /locus_tag="ACHOQV_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFP9631658.1"
                     /translation="MIKSTFWRALALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLSGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAKG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPTGWTLRDNGNKRELRNASGKL
                     VTEIIYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(5831..8149)
                     /locus_tag="ACHOQV_003338"
     CDS             complement(5831..8149)
                     /locus_tag="ACHOQV_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFP9631659.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVVLLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILMIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLGRLETFTTSLEKA
                     KKDGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVNPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALQQEIASYYPGGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAITLAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(8118..8723)
                     /locus_tag="ACHOQV_003339"
     CDS             complement(8118..8723)
                     /locus_tag="ACHOQV_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFP9631660.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLSDDERQRFAAQ"
     gene            complement(8720..9142)
                     /locus_tag="ACHOQV_003340"
     CDS             complement(8720..9142)
                     /locus_tag="ACHOQV_003340"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFP9631661.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVEEQSRELCFVSPDILFERMGVKP"
     gene            complement(9146..10822)
                     /locus_tag="ACHOQV_003341"
     CDS             complement(9146..10822)
                     /locus_tag="ACHOQV_003341"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9631662.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVAEQPGVTLIRLAENTGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKYATIGKRMDFDTEVMVRLYWQGNTCYFVPTRVTYPQDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTAARARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGHDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQRGKLHIHCETFADP
                     LLLPRGERQQGLQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(10813..11166)
                     /locus_tag="ACHOQV_003342"
     CDS             complement(10813..11166)
                     /locus_tag="ACHOQV_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFP9631663.1"
                     /translation="MIRHEIERHQVQLQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(11153..12511)
                     /locus_tag="ACHOQV_003343"
     CDS             complement(11153..12511)
                     /locus_tag="ACHOQV_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HFP9631664.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCILSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNNMNKRVYAQLQELFHD
                     TP"
     gene            complement(12508..13089)
                     /locus_tag="ACHOQV_003344"
     CDS             complement(12508..13089)
                     /locus_tag="ACHOQV_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631665.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFLIWLGLAHNSLHWLSPLMALL
                     LLLRLRQPRRQAGPLQAVTKIVAVVGIVLCVSSFLLKTHQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRCQMIKRKTP"
     gene            complement(13094..13345)
                     /locus_tag="ACHOQV_003345"
     CDS             complement(13094..13345)
                     /locus_tag="ACHOQV_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9631666.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVKRLLQEG"
     gene            complement(13357..13614)
                     /locus_tag="ACHOQV_003346"
     CDS             complement(13357..13614)
                     /locus_tag="ACHOQV_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFP9631667.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTNAPLFGDGLGLDSIDA
                     LELGLAVKNEYGVVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(13589..14410)
                     /locus_tag="ACHOQV_003347"
     CDS             complement(13589..14410)
                     /locus_tag="ACHOQV_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP9631668.1"
                     /translation="MNKLMSRLGWAWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVHGRVEINKFYDATSHEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(14407..15129)
                     /locus_tag="ACHOQV_003348"
     CDS             complement(14407..15129)
                     /locus_tag="ACHOQV_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFP9631669.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRYQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALMIESGDDWQCETQSAIAVNETTLPQSMLFLQH
                     YLQNAGAFSLSGERVQWRWSRR"
     gene            complement(15170..16228)
                     /locus_tag="ACHOQV_003349"
     CDS             complement(15170..16228)
                     /locus_tag="ACHOQV_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFP9631670.1"
                     /translation="MYEQDTLSTLDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEQTVLNEYAVSVLLDMGLSGRIITCKEGNYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDAARASWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFQAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(16292..16636)
                     /locus_tag="ACHOQV_003350"
     CDS             complement(16292..16636)
                     /locus_tag="ACHOQV_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631671.1"
                     /translation="MLSACARTAPVQQISTTVSVGHTQEQVKNAILKAGAQRKWIMTQ
                     VSPGVIKARYQTRNHVAEVRITYTATYYNIKYDNSLNLQASDGKIHKNYNRWVRNLDK
                     DIQVNLSTGATL"
BASE COUNT         3966 a         4439 c         4443 g         3826 t
ORIGIN      
        1 atacagtcgc cgtactggtt ccttccctga cagataaagc cagctcccgc tttatgcagt
       61 cattacagca ggtgcttaat aaaaatgaat tccagctact gcttgggtgt catgagtata
      121 atcagctcaa agaagctgaa attctgatga cgcttcttca aggcaacccg gcggcgttag
      181 ttatttttgg ttctcagttg gctgataaaa ctcaccagat actcgagaaa acaaatatac
      241 ctaccattaa cgttatcggt tcccctttca gtccggctca aattaccatc gaaacagcat
      301 tttttgaagc atcacataaa ctgaccgaac atctgctgga gcagggttat aagaatatcg
      361 gatttatcgg tgcgcatatg gacaaccgcc tgcaacgtca gcaactgaac ggctggcata
      421 aagcaatgct gagccattac cagaacactg atcttgttat caccatgccg gatgccgcaa
      481 gtctgcaatt ggggcgttac gcattaaacg agatattact gcgacaacca gagctggatg
      541 ctgtgatttg cagccacgaa gagattgcgc tgggtataat gtttgagtgc cagcggcgtt
      601 tgttaaaaat ccctggaaat atcgctgtag cttgtcttga cggttcagac aattgcgatc
      661 aaacacaccc aactttgaca tccatccgta ttgactacaa aaaaatgggg atagagacag
      721 gtaagttact gattggacta ttgaataata atcatgatga atcagaagag tcgcgtatcg
      781 tgcaattcaa ctaccagata gaattacgtc agagtacatg agcctaaaag agcgctatta
      841 caaagttagc tattgtggta gggatagccg tttccggcta ttccccgcgc agttccggat
      901 gtatgctgct aacgcatcca ttcctacttc aggtatttgg cgtagaagcg gatgctaaaa
      961 acttgtcgca aaaccgcaat atcagcctgc agtatcgctc tccagattat ccattcctat
     1021 attgatgaca acgacaggta agactgtgac tcaccaaact tggatgtttt tctgagctta
     1081 aagctcgttg cgtgaataag gcgagagtca gcgaatctca ttgaggcccg cagcaccggc
     1141 tgcaataagg atatagagca gcaatctacc gtcaacgtca aaaacacgga agccttgcaa
     1201 acgggatgcg ttcatataaa caacatggag cagcaacagt acgatgcctg ttgttgctca
     1261 gtcaggacct tttctgccat gcgcgagcca gcgtcacgat tgataaataa tgttcgcaac
     1321 tgattacttt actgcaacag acgtggttgg taataagcac tggcggacaa tgcaggatgg
     1381 aaggctgttt tcaggttgtc cgttcaggta cgttagatca gttcgttgcg ctaaagacaa
     1441 ctgttcagtc ccttatgtta cccgttatgg caaccattgc ccggtgataa ccgggcgtgt
     1501 catctgtgca tccgttgatt cgtgtggact gataaagttt tgtctgctga atcgttatac
     1561 ggaatgctct tcagtatgcg taagagaatt tgaggttcat attcattgat tcagattttt
     1621 tgaagaatca ccacatggga tgtccatgtg gttgtataac tcagggccag cgcttaataa
     1681 tgatggatgt attaatgccg ccaaaggcaa agttgttact ctgcaaaaac tcacaatcaa
     1741 ccttgcgggc ttcatgcatg atgtaatcca gcgcgccaca attcgggtct ggtttattca
     1801 ggtttagcgt aggcgcaaac caaccttcgc gcatcatttg cagactcatc caggcttcga
     1861 gcgcgccaca ggcaccaagc gtatgaccaa aataactttt cagcgaggag agcggcacat
     1921 tatcaccgta aatggttgct gtcgccagac tttctgccat atcaccgcga tcggtcgccg
     1981 taccatgcgc tgaaatatag ccgatatctt gtgcgcttaa ccctgcgatt tttagcgatt
     2041 gctccataca atactgcatg gtttcgcgct gcggctgggt aatatgtgcc gcgtcgcagt
     2101 tagtcgcaaa gccgacgatt tcaccgtaaa tggtcgcgct gcgcgctttg gcatgctcca
     2161 gttcttcgag aatgagcgta cctgcgcctt caccaatcac cagtccgtcg cggttttcat
     2221 cgaacggtga aggtgtggtt ttcggcgcat cattgtgctg gctggtggca aaaagggtat
     2281 caaacaccgc tgcttctgaa gggcataact cttcggcacc accggcaacc atcaccgttt
     2341 gataaccgtg gcgaatggct tcccacgcat aaccgatcgc ctggctgccg gaggtacagg
     2401 cgctggaggt tgggatcacc cgcccgcgca ggccgaaaaa gaggccagta ttaacagcgg
     2461 tggtgtgcgg catcatctgc acataggtgg tgccggtgat gttgttcgtg tgcttttcag
     2521 tcagcatggt ggcaaattcg ctcaccggac cggtactgcc ggtcgatgaa ccgtacgcaa
     2581 tgcccgtttc gccgctggtt aatatcggat cgccaatcaa ccctgcctgc tctaacgcca
     2641 gctcgctggc acgggtcgac atttgcgaca cgcggcccat cgcacggata cgcttgcggg
     2701 tgtagtgctc cggtagcgtg aaatcatcca caggcgcgcc aagcagggtg tgcagcccat
     2761 cgtagacctg ccactccggc attttgcgca ccgcattttc atacgccagt aagcgtgcag
     2821 aaacgtcctg ccagttttca ccaaaggcag tcacgccgcc cattcctgta atcactacgc
     2881 gacgtgtcat aacatccctc cgttgatgga aataacctgg cgggtgacgt aacccgcaat
     2941 atcagacatt aaataactgg caagcccggc aacttcttcc gcctggccca tacgtttcat
     3001 ggggatcatc gacattgcct ctttcagagc gctttcttcc atttcgatca tcccggtatc
     3061 aataagccct ggcgcgatgc aattgacggt gattttgcgc tttgcgagtt cgatagccag
     3121 tgcttttgtt gcgccgataa ttccggcttt ggctgcgctg taattcacct ggccgcgatt
     3181 gcccattacg ccggaaaccg aggacagcgt gatgatgcgc ccaccctggc gcgcgccgat
     3241 cattggcata atgcacggct gaatgacgtt gtagaaactg tcgaggttgg tgtggatcac
     3301 cgcatcccag tcgtcatcgc ttaaagccgg aaaagcggca tcgcgggcga tcccggcgtt
     3361 actgaccacg ccgtaccagg caccgtgttg cgcgatctca tgctccagca cttcccgaca
     3421 ctgttcgcgg tttgcgacgt cgaaactgag caaacgcccg ttacccccgt tggcgacaat
     3481 ggcattcagc gtttcctgag cgcctgtggc gtcacggtga tagtgaacgc caatgttaaa
     3541 gccgtctgcc gcaagcttgc aggcgatggc gcgaccaatg cctttgctgg caccagtaac
     3601 cagaactgaa cgactcatca ggacgctcct tgttgaaaca gggtggtaag ttcttccgcg
     3661 gtcggctgga aggtgttaat gcggcccgtc gcctcgccgt cgttgatgct gcactcaaag
     3721 ctgccgaagc gatcgtcctg catcagcagt ttgacggtaa tggtcagcgt ctggcctgcg
     3781 ggcagaatgc cagtggtgca aagcaattca cgcgcgccca gtatcatccc taactcaata
     3841 ctgtttttgc cctgctggtg gcgatgccag ccagaccaga cgccgacggt ttgcgccatc
     3901 agttcaaggg caaaccagcc agggagattg ccgtccgggt cgagaaacgg tgccagtacg
     3961 ccactgggcg aaaccgtcac gcggcacacc gcgctgtcat cgctgacgct caccacgtct
     4021 tccagcagta acatgggcgc gtcgtggggc agataagcgc cgggggataa atagtggctc
     4081 acgaaaccct cccgagcaga atgctggcgt tattgccgcc aaaggcgaag gaattggaca
     4141 ggatcaccgg acgtgccagc ggctgcggtt tttcgataat gccacacggt ggcagcgtgg
     4201 gatcgcgcgg cgacaggctg aagtcctgag cgggcagcgg taaatcacgc tgtaaaatca
     4261 acatactaat tgccgcctcg gtaatgccag ccgcgccgag cgtgtgtccg gtcaaatgtt
     4321 tggtggagct gcacggtatg cgtttgccaa acagggcgtt aaccaccatt gattcaatct
     4381 ggtcgttgag ttgtgtggcg gtaccgtgca ggttgatata gccgatgtca tctggcgtaa
     4441 gctgcgcatc ggtcagcgcc tggttaatgg cgcggatcgc cccttcgcct tgcggatgcg
     4501 gggccgaaat atggtaagcg tcgctcgatt caccaacgcc cagtaacgcg atgggctgcg
     4561 gttcacgggt taataacatc agtcccgccc cttcgccaat ggtgatacct gcgcggtcac
     4621 gaccaaacgg ctgacataag gttggcgaga gcgattcgag gctgtgaaaa ccgttaatcg
     4681 gcatccggct cagagtatct gcgcccccca caatggcggc atcgaccagc cccgcttcga
     4741 taagcctgcg cccgctgatc atcgctctgg cgctggaaga gcaggcggtt gaaagggtat
     4801 aggccgggcc gtcgagcgcc agccagtggc tgagaaaacg cgacggatcg ccgagttcct
     4861 gttgcgcgta gtgccatgtg gtgctcggct gtccgttggt gcgcaggtta acgtgcgtgt
     4921 cgccctcatg caggccggag gtgctggtgc caagcacaat cgcgatgcgc tcgcggccat
     4981 atttcgcgat agcgtcatca acctgcggct ggatctgcgc cagcgccgcc agcaggatct
     5041 gattgttgcg cgagcggtgc gcagcgaatt tttcggggat aagcggaagc tcgccatcca
     5101 cgccagccag gaccgcctgc ggatgcccct gcaaccagcc tgcgcgcgga cgcatgccgg
     5161 gggcgacacc gcgagccagg ttggctgcga tttcgtcaag attatttccc agggcgttga
     5221 tcatgccgac ggcggaaata taaatcatat cagtcaccta agtattgaat ggtgatgtgg
     5281 tatttaaaga catgctgctc aatgctgatt ggcacgcgtt tgccctggcg attcagatag
     5341 atgatttccg tgaccagctt gccgctggcg ttacgcagct cgcgtttgtt gccgttgtcg
     5401 cgaagcgtcc agccagtggg aagttgcggt tgccaggcgc taatcggcca gtggctgagc
     5461 attacgtcag ccagcacctg acttgcgggc ggcagctgcg ggacgacgat ggattgctcg
     5521 gcgcgtagac cttttgcatc gtaggtcacc agaaacaggc gaatgccgac cgacgacagc
     5581 ccggcaaggg tgattttctg gtcgtcggcg ttaagcatca ctagcagcga ctgggttttg
     5641 ccgttgaagc tgccgctcaa gagttgctgg gaattgacgg cgggtgaaat ccccggcgca
     5701 ggcagcgtga tgcgtgtgcc aggttgcagc cacgcctgcg ggcggccttc ctgttccggt
     5761 tgcgagtggc tacagccggt gaggataagc gtagcggtca gggcgagcgc tcgccagaag
     5821 gtggatttga tcattttttt gttctctttt tatcgggcat agccagcggc gaaagcagga
     5881 aggcggtgaa aataccgctc accagcacaa taccaaaact gctgatggcc tgggtggcgc
     5941 tgaataccag cattcccagc gtcagcaagg tggtgagcat tgccagcgtg atcgccagta
     6001 gggaagttaa cggtgtaccg cgcggattac tgaaaaacag cgtgtaattg atgccgatgc
     6061 caagcaccag caccagcgcc agtagcgaaa agagattcac cgcctgcccg ctcatcgcca
     6121 gcactgccag accgcagccc agcgaaagca ccgaaggcac caggctgata agccctttgc
     6181 gccagccgag gcgggccact gcgccgcagg caattactgc cagcgccacc agcaacaagc
     6241 cggttaacac ataacggtaa agtgcgaaca attcatcaaa ggtgcttttg cgatcaaccc
     6301 aggcaatgcc gccagggtaa tagctggcga tttcctgctg caacgcgcta cttttaaccc
     6361 cttcaaccgg caccagtacg ccgctttcgc cgttttccag cgtcagccac agcagacgcc
     6421 agccttcact ggcggggctt gccagccatt catcaacctt cactggcatg gcgttaagat
     6481 ccgggttcac cgccgtcagc ccggcatttt gcagtgcttt tgttaccgtc ggggcagccg
     6541 ttttcagcag gtctaaatct tcctcctgcc gcgccaggga gttcagcgga atggtgcggt
     6601 agttgctgat aaggccgtct tttttcgctt tctccagcga ggtggtaaat gtctccagcc
     6661 gtcccaaggt ttgctgtggg gagtcgccgt aaactacaaa ccatttttga tcgacgcttt
     6721 gtccggtcag ggcggtaatg gctttctcct gcgccagaat atgctgcggc agcgcctgta
     6781 actgcgagat atcgtcatcg acgcgtagca ttgacatccc cgccagcgaa aacagcgcca
     6841 gcgcgacggg cagacccagc gacagttttt tattacgccg ccacgccgcc agccagcgca
     6901 gcatcagcgc catcgccgga accgggcgta ccggcaggcc acggcacagc cacggatgcc
     6961 agaacaggac ggtcagacag gaggcgctca acccgacggc ggcaaaaatc gccatttgcc
     7021 gaataccggg aaaaggggcg agcatcataa tcagatatgc cgccacggtg gtgagcagcg
     7081 ccagtagcaa ggcattgcgc actttcgcca ggctttgcca cggcgaaacg tcgttgccgt
     7141 gcaccatccg ttcggtgagg taatagagcg tgtagtcagc ggaaatgccg ataacgctca
     7201 tgctcatcac cagcgtcatc aggtgtaatt cgccgaaaat caacaatgtg gcggccgttc
     7261 ccgccagcgc gccgatgccg atggaaatca cgcaaagcag caacgggcgt aaagagcgga
     7321 acaccgccac aatcatcaat atcaccccca gcagcgttgc cacgcccagg gtggagatat
     7381 cctgcttcgc ctgttggctg gcgtaatcgc tgtaaaacac cgtgccgcgc gagagcaact
     7441 gcgcctgcgg gtaacgcgtt ttcagatctt tttccagcgt attcagggtc gtgatgagct
     7501 ggtgggtttg ctgcatatca aacgacgatc ccgccagttc gccgtgcagc agataccagt
     7561 agttgccctg gggatcctgc gtcaccagcc agccgtccat cagtcgtaaa cgctggccgt
     7621 ttttcgccat cgccagctgc gagccgcgca ttaacatcag cggatcgttt tgcaactcct
     7681 tgccgcttac cccggagaac gcagaataaa gctgggagag gatccattgt gcctgcgctt
     7741 cgccgccgtt ttgcaggcgg gcgcgggtgt cggggtcaat caggccgttg cggtgctgcc
     7801 agaaaaacgc cccccacgcc tgctggctgg cggcatccat tggcccttta atgtcgccaa
     7861 gcgcagcgga cttttgcagc agcgtcagcc attcctgagc caccctggga ttagcctctt
     7921 taccggggct gaccagccac accagttggc ggtccagacg ctgcataaag ccgtcattca
     7981 gcgctggggg aatatcgccc atcgcctgtt tgggtaacat agccagcacg ctactgttca
     8041 gccgtgattg cggcagcagg atcagcaata ccaccgccat gaccaggcag actagccccc
     8101 ataacagcgc ggggcgttta ctgggcggca aaacgttggc gttcgtcatc gctcagttgc
     8161 gctggcgtca gttgatgttg ggtaagagcg atatcggtgc gatcgccctg tttatcatta
     8221 agctgaatgc tctccagata ggtttgcccg gcgagatcga tggtgttgaa aattttatcc
     8281 agcggcgtgg tggtcggcgt caggcgcagc gtccagcggc cttcgccttt atcggcaaat
     8341 tcgacgcgga agttttgttc cagcactttg cgatcggcct ggaacagcgc gcgcagcagg
     8401 tggttaaact ggaacatctg cgggttattt tctgccgtga tgatttgcgg cggctggccg
     8461 ttgatcacct gcaccatgcg tttatcgtcg agcaatagct gcatggggaa tggtgaagtt
     8521 tgatcccaca acagcccctg gtcgcgggcg atcaacatct gaccctgaga tcgcagcggc
     8581 tgcggcagat ctttaatcgt ccgggtttga tcgaaatggg cgcggatcac cggttgttcg
     8641 gtaaagcgtt gctgaagatc gtccagggtc agggcgctca caaacgggct aatcagcagc
     8701 gccagcagcg gtaaaaattt catggtttga ctcccatgcg ttcaaacaga atatcggggc
     8761 tgacaaagca cagttcacgg ctttgctcct ctactgcgac ctgaatggtg tatcctgtgg
     8821 tggcgcgttt gcccgtttcg gcatcgaaaa tctgatagcc aatccgcaga cggttttcga
     8881 actcttcgat atgcgcacgc acgcggatgc gttgctcaaa ggtgagggcg tggcgatatt
     8941 tcacccgcgc atcgaccact ggccacaaat aacctgactc tttcatctgc cgatagccgt
     9001 aattgaactg gttgagcagc gcctcgcggg ccacttcaaa gtagcgaaaa tagttaccgt
     9061 gccaggccac gcccatcata tcgacgtcgt gaaagggaat ggtcagctcg acttcagcgg
     9121 taaatcgggg atcgttaagc accctttact ccttgtcctg aatttccggc agttgccaga
     9181 aatcgaaaaa attaaaccag tcgaggggcg actggagcgc gtaatgttcc agacgcgcgg
     9241 cgtagtggtc gatagcgttt tgcagcccct gttggcgttc gccacgcggc aacagcagcg
     9301 ggtcggcaaa ggtttcgcag tgaatatgca gcttgccgcg ctggcgcagg gcgaaaatca
     9361 gattcaccgg gcagcgcaaa atagacgcga gaataaacgg cccctgtggg aaaggcgcag
     9421 gctggcccat aaacggactc cagcagacgc gccagtcgcc gccgcgttgc ggggtgacgg
     9481 cgatgcggtc accgacaatc gccacccatt cgccgttatc cagcttctct ttcagcagga
     9541 tggcggtttc cgggccgata tctgttaccg gcatcaggtt aatgcctgcc tggggagcca
     9601 tctcctgcat tatctgttta aagcgttggg cgttttcgct aaacaccagc gcgttaatgg
     9661 ttttgtagcc ctgaattttt gccagcgccc ggcacacttc cacatcgcca agatgcgagg
     9721 ccagcagcaa tttgccgcgc ggatcgcgga cgtcaagcgc cgcttctgcg cctggcgcaa
     9781 acagcacatc gtgccccagt tgtagctcgc cgcgccagct ggcgattttg tcgagcatgg
     9841 cattaccgaa acgtagaaag tgctgatagc tgttgagttt tgccgcctgt ggttgatgct
     9901 gtcgtacacg ggcgagccag tcttgcgacg ctttgcgcgc acgagcagca gtgagccagt
     9961 agacgcccac caccgggtaa agcagcgcgc taaacgccgt tcttcccagc agacgccaga
    10021 ccagcagcat caggcgcatt ccccataatc ctttcacttc actctgccgc gcccagtggc
    10081 tggaagagcg gcgcatcagt agtgaaggaa tacgcggcaa catgccgaaa aacagacgcg
    10141 tgtgcatgag cgagatgcgg acgttatctt tcagggcatc aaaatgcgaa agcccgtcct
    10201 gcggataagt gacgcgggtt ggcacgaaat aacaggtgtt tccctgccag tagaggcgca
    10261 ccatcacttc agtgtcgaaa tccatccgct tgccgatggt ggcgtatttt gccagttgca
    10321 gcgttggcgc aaccggataa acgcgaaagc cgcacatact gtctttcagt tgcagggaga
    10381 gcgtttcgat ccatacccag acgtgggtga cccagcgccc gtaaagccgt gagcggggga
    10441 tggagtcatc gtaaattggc tggccggaga tcagcgccgc agggtgttgt tcagcgagtg
    10501 ccagcagttt ggggatatct tcaatcgcat gttgaccgtc agcatccacc tgcaccgcat
    10561 ggctgaagcc cgcgtctgcc gccgcctgta agccgcgcat taccgccgcg cctttgcctg
    10621 tgttttctgc caggcgaatt aaggtcacgc caggctgttc ggcaaccaga ttatccagtt
    10681 gctgttgcgt ggcggcatcg ctgccgtcat ccaccacaat acagggaaga ttaaatggct
    10741 taagacgcgc cagcacgccc ggcatcatcg caccgtggtt gtagcagggg atcaacacgc
    10801 agggagaaaa gtttaccgac ataggcggat cttcccgcta ctggcggtgt ggcgggcgtc
    10861 accgtcgtga cgctgatagc agaaagtgag tacctgactt gtttcctgcc atgttaattg
    10921 cagggtaaca gtcgtctcag gtataagtgg cgccagaaat ttaacattct ggatactacg
    10981 aaaacgccag cccggagcaa gcagcgtggt agcataatgc attacccagt ccaactgggc
    11041 cacgcctggt aacagaggct gtacggcaaa atgaccatta aaccagtaga gcatcgggtc
    11101 aaggtataga acgatttcca tctcctgcag ttgaacctga tggcgctcaa tttcatggcg
    11161 tatcatgaaa taactcctgt aattgtgcat agacacgctt attcatattg tttactggta
    11221 tttcatcaat aatgcgccag taacgaggta ctgcaacagg ttcaagccat ggtaatagtg
    11281 ctttgcgcca ggacaactcc agagttttcc ctccggagca ttgccatatt tgacgggcct
    11341 ctttatttgg tacaaccacg acaccgatag cctggcggcc attgcgtatt aatggtattg
    11401 cagcgacatc cataataccg tcaagtgcca gaagccgttg ttcgatttca accagtgata
    11461 tccgcttctc ctctattttt accactcgac cacgtcgtcc gatcaggtgg aactgaccgt
    11521 tttcattgaa ttgcaacata tcgtcaagca ccaatccttc agcagggatg agtggcgaaa
    11581 agacacgaac cccctcactt tcctgtgaaa gatgaacatc agaaaaagta aaccagggga
    11641 tattatcctg ttgacggtaa cgccatgcga gaatacctgt ttcggtacta ccatagattt
    11701 catcgggcca gatattcagc caggtggaag ttttctctac atcctgccat ggtagcatac
    11761 cgccggcaga gagaatcatt tccacggggg gaggcgtcag atgaaggtcg agtcgtttaa
    11821 ggaacgccgg gctgctgaca aaggcatagc ggtaggtatg gcttaatgcg gcaagttgct
    11881 cagaatacca gagcattgcg gcatgcaatg gcaggccaag tgccataggt aaaaaaatac
    11941 ggaaagtcag cccatacagg tgctgaggca tcacggatgc tacaaaacga cagcccagta
    12001 gtcggtctgc aaagtgagtt gccagcatgt ttgcttcttg atcaaggctg ataacaggtt
    12061 ttgcaatttg ttttggttgt ccggttgaac ccgatgtgaa aagttcaata aacgcgtcag
    12121 aagcaatatc atcaaaggct atcgtgctgg tcgtcatctg gtgagatgtg cttacaacaa
    12181 actgtggacc gttccagccc agccttttat cactcaggat gcaatcaaac aaggagcgtt
    12241 gtacatttaa caaagcggcg cgattatggc ccgggattac tgggatttta cctgagtgta
    12301 gcgtggccaa caacgctaca ataaaaagat agctgttttc aaaacaaagt gcccagcgat
    12361 tgcctggctg gtcgcgtaaa tggtcgatca aaagcgctac atcatgccgc agatgcccca
    12421 gtgtccaggt atattcccca agccaggcaa caggagttgt tacagggcga gaggctgtca
    12481 accactgtga aagtgagaga gtctgattca tggtgttttc cgtttgatca tctgacaacg
    12541 tatcagccat tcccctgcag tcaggaaacc tatcagaagg taagcaatga taccgttcca
    12601 ggctgtccac aaaggcatgt tgccatacaa ggtggtaaat aacgcaatac tgccgttgaa
    12661 aataaaaaac aggcaccaga cctgcgttac gcggcgggta tatcgcacgg ccttttccgg
    12721 gaggtctggt tcctgcaaac gtgccagccg ctcaactatc ggcattgtcg accgcagcga
    12781 accgccaaaa accgccagca tgacagcgtt aaccactacc gggtaaaaca gcagtaactg
    12841 gtgcgttttc aacagaaaac tggagacaca caatacaatg cccacaacag cgacgatttt
    12901 tgtgacggct tgtagcggcc ctgcctgacg acggggctgg cgcaggcgca ataaaagcag
    12961 caacgccatc agcggcgaca gccagtgcag gctgttatgc gccaaaccga gccagattaa
    13021 aaaaggccag gcgagcagca ataatccggt cagcaactta atcatcggta gcgagcgaat
    13081 gcccgacatc aaattagcct tcttgtagca ggcgttttac agcctctaca acatcctgga
    13141 ctgtacgcac tgctttaaac tcttccggct tgattttctt accggttttc ttttgcaggt
    13201 gcacaatcat gtcaacggcg tcgatgctgt ccagttccag atcttcgtac aggcgcgctt
    13261 caggtttgat gtcctgcgga tcgatttcaa acagcttaac cagcagggct gagacttcct
    13321 gataaatggt ttgttggtct gtcatgatgg ctttcctcag gcacgttgcg cagcaatgaa
    13381 agaggccaga gtggcgacgg agaaaaagtg ctgacgcatt tcttcgcttt ccgcagagag
    13441 cacgacgccg tactcatttt ttactgccag ccccagttcc agtgcatcga tagagtccag
    13501 ccctaaaccg tcgccaaaca gtggcgcatt ggtatcaata tcatcaggag tcaactcgtc
    13561 cagattgagg gtgctgatga tgagattttt aatttccaga taaagcgctt gcattattta
    13621 ttcctgagag aggtaaacaa ccttgttgta attgaagcaa aagatgtcgg tttaactgcc
    13681 ttgcagccag cgccggctca tgtgaagttg catcgtaaaa tttattgatt tctacccgcc
    13741 catggacttc aaccgtaaaa aacggcctgg caggcggaac gtcataccat ttactttgtt
    13801 tatccagcag atgctcacta cagccgattg tcacgatgcg taaatcgctg gcgcagcgca
    13861 cggcaatatt cgctgcaccg cgctgtaatg tcattttttc accgggtttc gttcgtgtgc
    13921 cttcggggaa aatcaggata gtgtcaccct gtgccagtcg ctgttggcag cggggtaaca
    13981 gggtttcagc ttcgctgttg attaaataat ctgccgcccg cacgacgccg ccaagaaaag
    14041 gattttttaa cagcgcgctt ttcaccaggc agtcagtttc tggcatgact gacgccagca
    14101 gcacgtaatc gatcagcgtg ggatgattgg ctacgacgag gcaaccgcgt tcctgacgta
    14161 aaatttcagc gccatcaata cgataatcca gcacgccaag ccctttcgcg acagttaaaa
    14221 acaggcgaaa actggcggca atgctgcgac gcgccaaacg acggcggcgg gaagtatccc
    14281 ataccaaaac taacaatatg ttaaaccata cgactgaaag caacaggccg ccgagaccaa
    14341 acagggcaaa acataacccg gtcatgacca gtcgccatgc ccatcccagg cgcgacatca
    14401 gcttattcat ctgcggctcc agcgccattg cacgcgctcg ccagacagtg aaaatgcgcc
    14461 tgcattttgt aaatagtgct gtaaaaacaa catgctttgc ggcagtgttg tttcatttac
    14521 cgcaatggct gactgcgttt cacattgcca gtcatcgcca gattcaatca tcagcgcgac
    14581 ggcgtacggc caggtcggca tttctgcagg cagttgtggg tgataaaact cgggcagaaa
    14641 accgtcaaaa tcgaccatca acacgcgctg gtatcccgct tgtagcaggc taagcacttc
    14701 acacaaacct tgctggaaac tgtcgcgacc agctgaaagc gatgacgaaa cgatcggctg
    14761 tttggccgca atggtcaggt tacccacgga agaattgtgt acggaaagcg cgaagtccgt
    14821 cggagaaagc gcctgttcgg ttgccagtgc atgaacaatg cggtaattac gctccagttc
    14881 gccatgacga ctggtataca aaacggcgtc aggctgatag cggcgtagca ttgccagacc
    14941 gcattcaaca gccagtttgc tgcctgaact gagacgacgg gcagtcatca ttggcagttc
    15001 acttaatttt gccagcggcg cggcagggtc aatcgcccac ggctggcgcg accatgcttg
    15061 ccattgctgc acatcgctaa gtccaggcgc cagcgcctgc cagttggtaa tatttaatgc
    15121 aaatttcatc aacgcgtgcc cgcggtaaac caaaattatt aagtgacgat tatttctttt
    15181 ggcatatcaa aagtgtatgt ccaacgccta aattatcgtg gcgctctgct acctggaatc
    15241 ctgctttatt cagatagtcg taaaatttct ctgcactata gaaacggctg ttaccgtttg
    15301 ccatgcaggt aaaataaagt gaggacgcgt tcaggctaaa cgatgctgct tcaaaccgct
    15361 gcgcatccca gaacaactcc atgatgcaaa cttttgcgcc aggcttcatt acgctggcga
    15421 ttttgctgag tatgctaatt atttgttcgg gtgaaaaaca gtctaaaaat tggctcatcc
    15481 accagatatc agcctcgcct ggtaacgggg catcgcttag catatccacc gcttggaaat
    15541 ctatgcgatc agaaaaacct gcattcgcga tattttcttt cgccagtacg atttgttgtg
    15601 gcagatctaa taacgtaacg gcgatgtttt cattgtattt gcagcagcgc aaagcccact
    15661 tacccgtatt gccgcccaca tcgtacaagg ttgtggggtt gttggcgaat acataaggca
    15721 gtgcggcgtt aaatgcgcca tctgaatagt aatgatcaaa tgcaaaccag ctagcacgag
    15781 cagcatctgg taattgcgaa agtgccggat atatcgtcgg ccagtcgcca aatactttta
    15841 acccggaagg ttttccttca ttgagtgaat cggcaaggaa gaaaagcccc tggtaacaga
    15901 catcctgggt gaaatccata ttcacgcgag tcatagtgtc atgcaggagg taatgaccga
    15961 ttttcgccag gtaataattt ccctctttac aggtaataat tcgcccgctt aaccccatat
    16021 ccagtaacac actcacggca tattcattca ggactgtctg ttcagtaatc gcgttaaggg
    16081 gagctccctg tttaccctgt tgatccaaat aatctaatat tcctgcattg cgcaggcaca
    16141 aagctgcctg gaaaagcata ggagcaaaag caatccgttg tgcctccgtg atggcatcca
    16201 gtgtgctgag tgtgtcctgt tcgtacatat ataaccattc ttatagagaa gtttaatgat
    16261 atcgggctat tccagcccga tatcagcatc actataacgt tgctcctgta gacaagttaa
    16321 cctgtatatc tttatccagg ttacgcaccc aacggttata gtttttatga atttttccat
    16381 cagaagcctg cagattcagg ctattgtcat atttgatgtt atagtaagta gctgtatatg
    16441 taatgcgaac ctcagcaacg tgatttcgtg tttgatagcg agctttaata actccagggg
    16501 acacttgcgt cataatccac ttgcgctgtg cacctgcttt caaaatggca tttttaacct
    16561 gctcctgagt atgacccaca ctgacagtgg tgcttatctg ttgcactgga gccgtacggg
    16621 cacaggcact caacatactg ataaaaattg tgatggcaag ccattttata taac
//
LOCUS       DAZKGF010000111        16599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZKGF010000111 DAZKGF010000000
VERSION     DAZKGF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.111"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            227..574
                     /locus_tag="ACHOQV_003351"
     CDS             227..574
                     /locus_tag="ACHOQV_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFP9631672.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(592..1182)
                     /locus_tag="ACHOQV_003352"
     CDS             complement(592..1182)
                     /locus_tag="ACHOQV_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631673.1"
                     /translation="MTQSRQPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPATGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESITP"
     gene            complement(1182..1439)
                     /locus_tag="ACHOQV_003353"
     CDS             complement(1182..1439)
                     /locus_tag="ACHOQV_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631674.1"
                     /translation="MDEERVFSLSYEQLTRLAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            2036..2449
                     /locus_tag="ACHOQV_003354"
     CDS             2036..2449
                     /locus_tag="ACHOQV_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631675.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLDGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            2451..3230
                     /locus_tag="ACHOQV_003355"
     CDS             2451..3230
                     /locus_tag="ACHOQV_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9631676.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            3410..4054
                     /locus_tag="ACHOQV_003356"
     CDS             3410..4054
                     /locus_tag="ACHOQV_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9631677.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            4141..4449
                     /locus_tag="ACHOQV_003357"
     CDS             4141..4449
                     /locus_tag="ACHOQV_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFP9631678.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            4862..5842
                     /locus_tag="ACHOQV_003358"
     CDS             4862..5842
                     /locus_tag="ACHOQV_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFP9631679.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGADIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            5835..6251
                     /locus_tag="ACHOQV_003359"
     CDS             5835..6251
                     /locus_tag="ACHOQV_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFP9631680.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(6253..7527)
                     /locus_tag="ACHOQV_003360"
     CDS             complement(6253..7527)
                     /locus_tag="ACHOQV_003360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP9631681.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAIEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(7527..7964)
                     /gene="umuD"
                     /locus_tag="ACHOQV_003361"
     CDS             complement(7527..7964)
                     /gene="umuD"
                     /locus_tag="ACHOQV_003361"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP9631682.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLHPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(7961..8209)
                     /locus_tag="ACHOQV_003362"
     CDS             complement(7961..8209)
                     /locus_tag="ACHOQV_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9631683.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSLSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            8627..9529
                     /locus_tag="ACHOQV_003363"
     CDS             8627..9529
                     /locus_tag="ACHOQV_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP9631684.1"
                     /translation="MYFSAEPAQITEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIKVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            9526..9837
                     /locus_tag="ACHOQV_003364"
     CDS             9526..9837
                     /locus_tag="ACHOQV_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631685.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLPSKRRRLRYSTVGLTRYRT
                     R"
     gene            9913..9984
                     /locus_tag="ACHOQV_003365"
                     /pseudo
     CDS             9913..9984
                     /locus_tag="ACHOQV_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10066..11767)
                     /gene="ltrA"
                     /locus_tag="ACHOQV_003366"
                     /pseudo
     CDS             complement(10066..11767)
                     /gene="ltrA"
                     /locus_tag="ACHOQV_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            12389..13000
                     /locus_tag="ACHOQV_003367"
                     /pseudo
     CDS             12389..13000
                     /locus_tag="ACHOQV_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            13001..13222
                     /locus_tag="ACHOQV_003368"
     CDS             13001..13222
                     /locus_tag="ACHOQV_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631686.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            13182..13668
                     /locus_tag="ACHOQV_003369"
                     /pseudo
     CDS             13182..13668
                     /locus_tag="ACHOQV_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            13713..14483
                     /locus_tag="ACHOQV_003370"
     CDS             13713..14483
                     /locus_tag="ACHOQV_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631687.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDIEQLNPHYPGWISIYVR
                     LDVSRLATLLISRHDGVLPPVLASAMQKLTGTVAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVCECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            14896..15321
                     /locus_tag="ACHOQV_003371"
     CDS             14896..15321
                     /locus_tag="ACHOQV_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9631688.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            15368..15787
                     /locus_tag="ACHOQV_003372"
     CDS             15368..15787
                     /locus_tag="ACHOQV_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP9631689.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIARDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            15787..15978
                     /locus_tag="ACHOQV_003373"
     CDS             15787..15978
                     /locus_tag="ACHOQV_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631690.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            15992..16293
                     /locus_tag="ACHOQV_003374"
                     /pseudo
     CDS             15992..16293
                     /locus_tag="ACHOQV_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16293..16388)
                     /locus_tag="ACHOQV_003375"
                     /pseudo
     CDS             complement(16293..16388)
                     /locus_tag="ACHOQV_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3656 a         4222 c         4470 g         4251 t
ORIGIN      
        1 cattttagta tctgagtgcc atgtggttga tattttttct ggtgcgggaa tgatgaaatc
       61 tgctatcata atattaagaa aatactttac agatatatcc agtttcatct ggtttctcct
      121 ttattgttat cgtgtaccgg tgcatgacaa cataatcaca gcatgataat aataatcaat
      181 aacaataagc tgtgtcacgt ttacatcatg gaacggaggt gctcctgtga cctttgagca
      241 gaaaaaagcc cgggccattg ccctgatgga tagcaaaaag atgtggcgga gcaattatgc
      301 ccctcctctt ctgcgtattc tctggcggct gggtatccgg cttccgccat taccctttat
      361 gccgttctgg caggtcacgc tgttgatggg gagcttatgg ggcatttcct ggggatgtgc
      421 aatgtggttt atctactggg ggccgtcagg aatggtggca ggtgaggcca ttattataag
      481 tatcacgggt ggttttttgt tcggactgct cacggcctct ttccactggt ggcgcaggaa
      541 agttaaccgg ctgccaccat gggatgatgt gtaaaccgtg caggcatgtt tttacggggt
      601 gatgctttca ttaacctgca cacggacctc cattcctgca tcgtgacagg cctgcagcca
      661 ctgcgccact tccagcggat cgccctcccg gcgtaccact ctgccttctt tattccataa
      721 ctgcagacag gtgctgccgt cgagacgcac cacaaaatcc ccacggcagg cctgatacgt
      781 tatgccgttc agctccaccg ccagatcagt ggcagaagtg ccgtctgcgg gcagacctgt
      841 tgccgggatc agcggcagta cgacgacttc tgccgcacgc tgttctgccc ggaggcggtc
      901 ctgttctgcc agcagcagag gctgtgccac ggcccggccg gcgtccgtca gctccactgc
      961 cagctgcagg ttcggagcgc gcagggtacg cagccagccg gcatcctcca gacgacggca
     1021 ggaggcgcgc aggtttggcc cgtagaccgg ggcttccccg cttcgctcca gcacccgttc
     1081 tatgtcccgc gtcagcacgg ggcctggccg tttttcatca agggcagcca gcacaatcag
     1141 cactctccgc tgcagtggcg acggttggcg gctctgggtc attacctgtc atcctccggc
     1201 cagatgaggt ttctgaggcg ctgaaagtcg gcgtcaatga gctcctgtct ctttatggca
     1261 ttcattgagg catagccatt tatggccagt tcatgccaga agatcagcac ggctccggct
     1321 ttggctgact cgcagagata aatcgcgccc tggctgtcca gattgcattc ccggatgcgt
     1381 ttttccgcaa gccgggtcag ttgttcatag ctgagtgaaa acaccctttc ttcatccatc
     1441 ctgcgcgctc catctgataa tgttctgaca ggtaaaaatc aaattctata tgatttttgt
     1501 gttattgatt tctgataata tcagatattg tttcattacg gatgattaaa atcatgtctg
     1561 atatgaattt atgtgtgttg cttaattatc atatcgcctg cccgtttccc gccgcgcaga
     1621 tccggtttcc ggcttttctg ccgcgcagat ccgaaaaccc tgagtcacgg atcttcctct
     1681 ccctcacaca acgccatctc ctgtcagtac aacatgtcgt gctgcattca gctgctcatg
     1741 acactatata ttgtgttatt tccctgacct gtgatgcgtc gcgcaggggc ggaaaacagc
     1801 gatatgatta tttccccagc gtggtacact tccggaaagt tgtgatattc aggaaagtcg
     1861 gatctgacgg aaacggctct ccggtaattt aacggcgtgg ttatatggat gcttgttatc
     1921 atggtgatga tgataacggc atgatgttat cagacggcgt gacggtaagg gcagtgatga
     1981 tggatgacgt tatcgcatga ccgtccctgc ccggaaaaga aaaaaggagt cacccatgtt
     2041 ttttattgag aatgaaggtc aggctgtcgc cagaacggat tactggcagt ctgtacaggc
     2101 gcaggccgga tatgtctacc tcagctggaa tgccggcgca gccaggctgc ttgtcccgga
     2161 tgcggcaaaa catttactca gggagatgcg gggggctgag tacgtcatca tcagtaaggg
     2221 aacgctggat ggccgcgatg cgctggaact gatatttgaa gacggcagcg atgcgccgtt
     2281 tgtgatccac atgctgagtg agcagtgcga tcgcctgctc cccgaaaaca accagggagg
     2341 gggttttgtt gtcaccgtct ggacgcgtgg cggtaaccag ctccgttatc cgggaaaata
     2401 ccgggttgtg gaaaacctgc ccgacgtttc cccgtggagt gagcactgat atgcagcacc
     2461 tgccggcacc gatccaccat gcccgggatg ccgctcagct tcctgttgcc atcgattatc
     2521 cggcagcgct ggcactccgc cagatgtcga tggttcatga tgaactgcca aaatacctgc
     2581 tggcccctga agtgagcgcc ctgctccatt acgtgccgga tctgcgccgc aagatgctgc
     2641 tggccacact gtggaacacc ggtgcgcgca ttaatgaagc actggcgctg acgcgggggg
     2701 atttttcgct tgcgcctccg tatccgtttg tgcagcttgc gaccctgaaa caacggacgg
     2761 aaaaagccgc caggacggca ggaagaatgc ccgccggtca gcagactcac cggctggttc
     2821 cgctctccga cgcctggtac gtcagccagc tgcagacgat ggtggccaca ctgaaaatcc
     2881 ccatggaacg gcgtaataaa cgaacaggca ggacagagaa agcgcggatc tgggaagtga
     2941 cggacagaac ggtcaggacc tggattgggg aggcggttgc cgccgctgct gctgacggtg
     3001 tgacgttctc tgtcccggtc acgccacata cgttccgcca ttcctatgcg atgcacatgc
     3061 tgtatgccgg tataccgctg aaggtcctgc agagcctgat ggggcataag tccatcagct
     3121 caacagaggt ctacacgaag gtgtttgcac tggatgtggc tgcacggcac cgggtgcagt
     3181 tttcgatgcc tgagtccgat gcggtgtcta tgctcaaaag aattccttaa gctctgactt
     3241 ttttaaatcc ctttcggata tctcatggat atattatcgg tatttcatat tctgttgaca
     3301 tgcaggaccg gtatagagta aaaaaacatc tattgagtac ctcttggata tccgaaaggt
     3361 gtcttagggg gtctttatgg gtattacatg tggattctgg agatacacta tgcctgttat
     3421 tgctaatacg catcccaaag gtggtgtggg taagacaact tcatccgtaa acattgttgg
     3481 tgaaatgaag tctgatacag ttgatctgga tactcatacc gggctttcta tcattctggg
     3541 gctgagacct gaaggaaaag aaatttccgt gaaagtaccc aaaacagtgg atgagttaat
     3601 cgaaattatg actccctaca agaacagcga taagacgtta cttattgact gtgggggatt
     3661 tgactcagat cttacccgta ctgcgattgc ctttgcagac tgcgttattg tcccttcaaa
     3721 agactccctg actgaacgta ttggtctgat gcattttgat ggagtactgg acgaaatcag
     3781 ctctatcatg gggaccgata taactgctca cctgtatctc tgtaaagtta atcctaacaa
     3841 gaagaaattc cctaagcttg atgcaatcct gccatcgttc aaacatctga agctgatgaa
     3901 gagccgcatt tctgctcgtg cagaatttga ggatgtcatt gagactggga tgggaatcac
     3961 tgagtctgtt catgggcgct attcggcagg aggtaaggaa gttattgccc tgatcgaaga
     4021 aatcaatcat ctgattgaaa ataacaaaca gtaaggtgta tctattgggt attcatatag
     4081 tatcctttgg gcttttaatg gatatctttt aagtatctaa caaacatcct gggttatctc
     4141 atgagcaaag cgaaatttag tgcacaggct gaaaaactga aaaacctggt ggattctgcc
     4201 agattacaaa cttcttcttc tgccgtaaca gcaacagccc cgtctcagcc aaatgaaggt
     4261 gcagaggaag tttcaggtcg tcccgtggct gccaggacga aggctaagaa tcttaaggcc
     4321 ataccgctgt cgtattttga agctcatgcg gaacttaaag cgaccagtaa aacatctctg
     4381 gattttagct catatattat tgaggctata agagagaagc ttgaacgtga tggtgcaata
     4441 ggtcaataaa ctatcggata ccttaagggt atctgatgtg tgtcttttaa atgcgttatg
     4501 gggattcttc acgtacctaa agagtcccca ttagacatct ttctgtttat ggtgacatga
     4561 ttcgtcaccg ctttcctttc ctctcagttt caccatttca ttcctttccc ttaacctgaa
     4621 ccatgatgtc cacgatgttt ttatattgca tttatgtatc acatataacc ttcataatgt
     4681 ttttgtatct atgatttcct ctggtcttaa agttgtgcgt acaatgtaca tgcaatttat
     4741 cgtgttttta tgttgcattt gtcctttcag aaagaccgtt ttttcattat aattgaatat
     4801 acattgcatg tatgaagcat gtttgttttt tctatttgtt tatgagcaac ggagactggt
     4861 tatgaaaatc tttattgatg atggttctac caacattaaa cttgcctggt tggaggatgg
     4921 tgacgtgaaa acgttaatca gccctaacag ctttaaaccg gaatggtctt ttagtttact
     4981 ggatgatgct gctccggcta attacgagat cgacggcgag aagttttcat ttgatccatt
     5041 aagtgctgat gctgtcgtaa cgactgaaac acgctatcag tacagtgatg tgaatgttgt
     5101 ggccattcag catgcgctac agcagaccgg cctgaaagcg cagcctgttg atgtcatcgt
     5161 cacattgccg atctctgaat accttgacgc taacaatcag aaaaataagc aaaacattga
     5221 aagaaagaaa aaaaatgtga tgcgtgaagt acgggtgcag gggagtgatg catttgttat
     5281 tcgcagtgtt tccgttcttc ctgaaagtat tcccgctggt ttcagtgttc ttgcggggct
     5341 tgaggatgat gaatcccttt tgatcgtaga tctcggtggt acaactcttg atgtgtcgca
     5401 tgttcgtagc aaaatgacag ggattacaaa aacctggtgt gatccgaata tcggcgtgtc
     5461 cctgattaca tccggggtta aggaacagat ggctgtacat gctaatacaa gagtgagctc
     5521 attccaggct gacaatatca ttgtgcaccg taatgaacct gactaccttt cccgtcgcat
     5581 ttataatgct gaacagcgtg agtctatcat taatgttatc aatgaacggc agaagttgct
     5641 tattaaacga gttaatgatg tcatcagccg tttcaccgac tatacacatg taatgtgtgt
     5701 tggtggtggg gctgacattg tggccgaggc tgtgaagaac ctgactaagg ttcctgatga
     5761 gcgcttttat ctgagctcat ccccgcagtt tgatttagtc atgggaatga taaaaatgaa
     5821 aggtggtgtg actaatgagt gatgaaaata aatccagacg ctgctcattt gaacttttcc
     5881 cggatgaacg gacaggagat aagatagcag atgagctcat tgcaaatgaa aaactgaagg
     5941 agcggggacg tttcatgcgc gcgatgttag ttactggtgc ggcgtttgca gctatagata
     6001 aacgtcttcc tttgctgata tcggagttac tgacagaaaa tacgactctg gatgatatta
     6061 ataaggtgat ttccagcgta ataccaggtg ctttttcggt tgaaaagaaa ttgcttgaat
     6121 tactggaaaa acaatctggt ctccatacgt ctgtggattg cagtactcct cttacagagc
     6181 agagtctgtc gagaaatgac ggtgaagacc agaccagaag aaacgctgaa aatatgttcg
     6241 gagatgactg aactaaccca gcctggcaag gggaatatct cgccatttag ttgtataaca
     6301 ctgactcaac atttcccgtt tcatttgcca ttcaggggcg gttcctcgcc ccgcaaacca
     6361 cactttccct tttcccgact ggttcagttc atcgagtgtt ttcattaact tttcactgtt
     6421 tttacggggc tggatttcat caaataatcc cggctgcgct atacccgatg gtgtgaaatc
     6481 agccagcatg actcctgcct tcatatagcg gtacccttca cgccagacat ggtttaaggc
     6541 tctgcatgcg gcggcaataa tgtcccggct gtcctgtgtg ggcaatggaa gcttttccac
     6601 agcggcattg ctgtaacagg gttcttttac tgcaaagggg gatgtccgta caaatgtcgt
     6661 cacctgccgg caatactgac gctccccacg tagtttctct gcggcccgct ctgcatactg
     6721 aacaacagcc tggtgcatgg catctttgtc tgtgattcgt tcaccaaaac tgcgactaca
     6781 gacaatctgc tgttttgccg gtggtgcttc ttccagggat atgcaggact cgccgttgag
     6841 ttcgcgtacc gtacgctcaa gaatgacgct gaagtttttc cggatgaatg ccgtgttagc
     6901 ctgcgccagc tgcagtgctg tgttaatacc cagcgcattc agcttttccg tcagtctgcg
     6961 tcctactccc cagacctcac caactggctg cagccccagt agcttcaagg tccgattacg
     7021 gttttctgcc gtcagcgcga ccactccgga aaactgtggc cattgctttg ttgcccactg
     7081 tgcactttta gccagcgttt ttgtaggcgc aatgcccacc cccatggtga gtcctgtcca
     7141 gctctttacc tgttccctga gctgatgacc aaaaaactcc ggagagatgc aatgatttat
     7201 cccccgcaaa tcaataaaca tttcatcaat tgagtagggc tcaattgcgg gagaaagcga
     7261 ctccagaaca gccatgaccc gttggctcat gctgtggtac agcgcataat tgctggaaaa
     7321 tacatgtatt ttcttctcca ggcgcatctg tctcacctga aaccagggct gccccattct
     7381 gatgccaagg gcttttgcct ccgggctgcg cgcgatcaca cagccatcgt tattgctgag
     7441 tacgatgacc ggttcgttgc gaaggtccgg gcggaaaact ttttcacatg aggcgtagaa
     7501 actgttgata tcagccagtg caaacatcag cgtaactccc gcgtcctgtg tatcacgtga
     7561 gtgacaacac caaaaataca gatgttttcc ggatacagtg tgcggaactc cgggctgtct
     7621 gaaaccggct ccagtgccgg gcgtgggtgc aacagcagtc gtttgacggt gaactccccg
     7681 tcgatctcag cgataacgat gtccccgtgt tgtggttttt cggccctgtc cactaccagc
     7741 agatcaccat tctgcacgcc agcctggttc atcgattcac cgctggcgcg cagaaagaag
     7801 gtggctgcag gtctgctgat gcaatagctg ttcagatcca gttcctgctc agcataatca
     7861 gtggcaggag aaggaaaacc ggcctggcaa cgatcggcaa acaacggtcg cacgtaactg
     7921 tcatcgcctg aaggggcagc cggacggtga taaacggtac tcatgctgca ggcagccagc
     7981 tgtcgtcctg ccagatttct tccagcagag acaggatacg ttctttatcc ttgtcgttgc
     8041 gggctcctgt gacagataga gacggggcgc tgccctggtc tatacgcagc cagatttccg
     8101 ggtagtgtgg tttcagacgt tgttcgattt cattctgtag cgcctgaagt gtgcctgatt
     8161 tgaggttttt tgtgctctgg cggtcgaaaa gaatttcaat gcggatcatg ttctcatctc
     8221 ccttttcttt tgctgtatat gtatacagta ttctaataac tgaatccgca cacagtcaaa
     8281 tgtcattgtt ctgactaaaa agcgatcaat tctctgaggg ggtggctgta tgcctgctgt
     8341 ggagtgctgt atccagctta tccacagcat tttgtgcacg gttctgtgga caaaatacct
     8401 ggttacccag gccgtgccgg cacgttaacc gggctgcatc cgatgcaagt gtgtcgctgt
     8461 cgccggcctc ctcacccggt cacgtttcgt cgttcctcct ccacgcgctg cggcttcggg
     8521 gccgcacctg cattcgtatg cggtcgcccg gttacaggtg cggcacggcc tgatggaggc
     8581 cgcatgtgag aggagaattc ctatgccaaa ctggtgctcg aatcgtatgt atttttctgc
     8641 cgaaccggca cagattactg agattaaacg actggccagc ggtgcagtca caccgcttta
     8701 tcgccgcgcc acaaatgaag gtattcagct gtttctggcc ggaagtgccg gacttctgca
     8761 gaccactgaa gatgtgcagt ttgaaccgtg ccccggactg acggctgccg gacgtggtgt
     8821 tgtatcgccg gagaatatcg cgttcacccg ctggctgaca cacctgcagg acggtgtact
     8881 gctggatgag caaaactgcc tgatgctgca tgaactctgg ctgcagagcg gtactggaca
     8941 gcgtcgctgg gaaggattac cggacgatgt cagggatacc atcaccgcac ttttcaccgc
     9001 aaaaagaggt gactggtgtg gcttctggag taacgaggat gtatcggtgt ggtggaaccg
     9061 tctgtgtgac aacgtactgc cggaaaaaac catgccgttt gacctgctga cggttctgcc
     9121 gacccgcctg gatgttgaag tgaatggctt taacggtggt gttctgaacg gtgttccttc
     9181 tgcatatcac tggtatacgg aacagtatgg cgtgaagtgg ccttgtgggt atgaggtgaa
     9241 tatcagcagt cagggagaca acttcattaa ggtggatttc gacacgccgt ggtgtcagcc
     9301 ggaaagcgac gttattgcag aattaagccg ccgtttcagc tgcacgctgg agcactggta
     9361 tgccgaacag ggctgtgatt tctgtggctg gcagttgtat gagcgcggag agctcgttga
     9421 tgtgctgtgg ggggaactgg aatggtcttc cccgacagat gacgatgagc tgccggaagt
     9481 caccggacct gcgtggatag tcgacaaggt ggcgcattat ggcggatgaa gtatgacgga
     9541 gacgggcggg caaccgcccg tttcttttcc ggtaaaggat gtcgccgtgc tcctcttttt
     9601 actgcgccgg ctgacgcggc gcggcataaa cgctgcctgt ggtcagtgtc ccgcgtccgg
     9661 cgggcacggg acgggcgctt ttaccgctcc cggctggcgt cggtgacagt gtacgccagc
     9721 ccgtcgccct tttctgatga acgcccttca agccgctttc gcggcataac cttgccgtca
     9781 aaaagacgac ggctgcggta ttccacggtc ggcctgaccc gttaccggac gcggtgaaca
     9841 gcccacaggc agcggggaac gggcaccaca ggggtgccct cccggagccc tctgtaagag
     9901 aaggagtttt gtatgtcccg ttttgtcctt ggtaactgca ttgatgttat ggcccgtatt
     9961 cctgataacg ccattgattt catgtcgggt agcagctgca cattactgtg cagccgcccc
    10021 ctaagaaccg tacgtgccag tttcccggca tacggctcaa gcctttcaat aactccacat
    10081 tagagccggc ttcataacag atattccctg atgttttgca gtgtgatgac atgccggatg
    10141 taacatggcc agattaccta tgttatccag tcccccttcg gatttcggca caatgtgtcg
    10201 aagttgccag cctgttgtgg cgttcactgg caggtggcag acggggcact gacctccctg
    10261 tcggagccac agatttctta ggcgagagta accctgtatg gattccagca tcgtagcttt
    10321 tagccgtgct tcaaagtacg attcgtagct gggatcaaaa gggttagcag tgctttttat
    10381 ttgtctgtgt cttttgactg gcatatccga gatttcatat agcgccagta actcacgttt
    10441 gcctgatggc aggagcttcc ctgtttctgt gccgaatacc cagtgtcgga agttaactga
    10501 acgaaagtat ttctttctta tccagcctgt acctttcttt gggtgtcgtc gacatgccca
    10561 gcgccatagt gctttccata tttcatgact gactttctga aagatctttt ttgaaacaac
    10621 atgacgatag tatctgaccc agcctttgag gactgggttg agatgccata tcagtgtgct
    10681 ctggctgact gtcggattgt cttttatcgt ctggcggacc cttttcagga attcctttac
    10741 cttttggggt gaaggtttaa tcaacagttt cccgttatat ttgcggatgt tcatacccag
    10801 aaagtcgaat ccatcactga tgtgtgtgat tcgtgtcttt tctggtgaga gggtaagccc
    10861 tctttctgcc agaaagtttt ctaacaaggg aagaaccttt tctgacagcg tgtcgtggct
    10921 tatccccgtg atcacaaaat cgtccgcata acggattatg ttgatctttt ctggattcag
    10981 tccgcctcga tagaaggttt tcttcaacag aggctgcaga ccatccagaa ccatgtttgc
    11041 cagcgttgga gatattattc cgccctgtgg agttcctgca ctggttgggt ataatttccc
    11101 caaatcaata tatccggctt tcagccatcg tttcaggatc gccgtatccg ttgggatatt
    11161 ggcgattagc cattcatgtg aaatctcatc aaaacaactg cgaatgtcgg cttccagaat
    11221 ccattgtgcc cgatcagccc tgtgcaacgt tgtgaaacat tgctcaatgg catcggctgt
    11281 tgagcgacag ggcctgaacc caaacgaatt tgggtcagcc gttgtttctg caacaggttc
    11341 cagcgccatc agatagagtg cctgcatggc tctgtctttc attgtcggga tccctaatgg
    11401 tcgctttttg ccgttagatt ttgggatata tatacgccgt agcggaagag gtttgtaacc
    11461 cccttctttt cagcgtgtat atagccttca tcttgctgcc gggttttgac catgttttgc
    11521 catcaacacc cggcgttctc ttccctctgt tatctgtcac tcgtttaact gccattgctc
    11581 tggcgctgaa cgagtgggtg agtagccatt gcaaggcttt taccttgccc gctttctcct
    11641 cctgtattgc cttcacgata cgcgcctgca gccttctgac ctcgcgacgg cacttggccc
    11701 agttgatatt gtgccagtga gtcacgatgt cggtgggtgc acccgcgttt atgtttttcg
    11761 cagtcatctg cttaccctcc ttcaaaggta ttcaggttat ctcgtgttga aagaccatgc
    11821 ggaagtctgc ccgctttcgc gtggaatgat gttgctgaaa cagctcaact cctgtccatc
    11881 ccattacagg atggcattcg ctttctccgc actcctgtac ccgcatcact gacagctttc
    11941 ctcgcggtta gcctgccctg agggcagtga tacgggctta ccgtgttccg cacagtttgc
    12001 agggatgggt taggttctgc ctatccaccg gtagctttga tgtccgtgcc tccccagtca
    12061 aaagaggaag gtccagctac gttcccattt tgggtcgggc ctgtcagtgc ctttggcccg
    12121 ttcagggttg acgatgttta tcagcagttc acatgtgtta accctgccat ccagccttgc
    12181 gccccatccg ccctgacact ggcagacatg cttcctcctc gcggattcca catggtgtga
    12241 aaaccgggct acgttgtccc gaaggcttca taccgaacag ttacccgctc tgcatgcttc
    12301 ggtaggctac aggcgacgga acgcctggtt ttactacttc ctttacatga agttaaaaca
    12361 acaaactgtg cgactttcac gtcgcaccct taccgatcct ccatatctcg tcggtttccg
    12421 tgaccgtcag gggcgcacca ttgccggtga cgttaatgat gactggctgc aacctgccag
    12481 caatgaaatg taccgcgtac tcaaaaaaga tgcgctgatg gtgagtttct atggctggaa
    12541 ccgtattgac cgctttatgg cagcctggaa acgcgccgga ttcagcgtgg cagggcacct
    12601 tgtgttcacc aaaaactaca catccaaatc tgcgtatgtc gcgtaccgcc acgaatgcgc
    12661 ttacatcctg gcaaagggca gtccggcccg gccccgtaac ccgctgccgg atgtgctggg
    12721 ctggaaatat tcgggcaacc gccatcaccc gacggaaaag cccgttacca gcctgcaacc
    12781 actgattgag agcttcacac atccgaacgc gattgtgctg gacccgtttg caggcagcgg
    12841 ctcaacctgc gtcgccgccc tccagtccgg acgcaggtat atcggtatcg agctgcttga
    12901 gcagtatcac cgtgccggac agcaacgtct ggctgccgta cagcgggcca tgcagcaggg
    12961 ggccgcaaat gatgactggt ttatgccgga ggctgcgtaa atgaactatg ccggacatga
    13021 aaaacttcgc gccgaagtgg cggaggtggc caatgccatg tgcgacctgc gtacaaccat
    13081 gaatgagatg gagcggcggt acagctttaa tgctgacacc ctgccggaac gtctggtgcg
    13141 tcagacgttg tttcgcgcaa accgcctcct gatggaggca tataccgaaa ttctggaact
    13201 ggattcctgc ttcaaagatt gaggggagag agtatgtacg gaacatgcga aatactctgc
    13261 cgggagctgg cagcaaagta tccgggagac acgccgctga tgctggttat ctggtctccg
    13321 gaagagattc aggcccttgc tgacggaatg gatatttccc tgtccgatca tgaaatcagg
    13381 accgtcctgg cgcgcctgga ggacatcccg gaagaccagc ggactgagtc cggtatttct
    13441 tcgggtgcgg ctatggagat tatcagcaac gtgagtgaaa accgccaggt gaccgtccct
    13501 gctgaactgc tggcgtccct gattcagacc gctgaacagg cattgtggaa gcgtgaatgg
    13561 gccgcccgtg ataacggcct cgtcgtcccg gaatgcgtca cccgccgtca ggcggtggtt
    13621 aatcaggccc gcgccctgct gaaaaacaac acacacgaaa acgactgatg ttatcgccgc
    13681 tttcgggcgg cgattcaggg agaccattaa tcatgaacaa aacgctgaat gcactggttt
    13741 gtcgccacgc ccgtaacctg ctgctggcgc agggctggcc ggaagagacg gatattgaac
    13801 aactgaatcc gcactatccg ggctggatca gcatttatgt gcgactggat gtatcccggc
    13861 tggcgacgtt acttatcagc cgccatgatg gcgtgctgcc gccggtcctg gcgtctgcca
    13921 tgcaaaaact gaccggaacc gtggcggaac ttgtactgtc cggcagccag tggcaggcgc
    13981 tgccggtact tccggcagac ggaacgcagg tgtctttccc gtatgccggt gaatggctga
    14041 cggaggaaga aatccgggca gttcttgatg cggtgcgcaa tgcagtacga agcatctgtt
    14101 accaggtggc agacgatgcg cggcgtatcc gggcggcgct gaccaccacc ggtcagacgt
    14161 tgctgacccg ccagacgcgc cgctttcgtc tggttgtgaa ggaaagcgat tacccctgct
    14221 ggcttgatga agatgacgaa aatctgcccg tggtgctcga tgccattctg aaccggggcg
    14281 cccgtttttc ctctgtggaa atgtacctgg tctgtgagtg tgtggagcat atcctggcca
    14341 gtggtctggt ctgtgatgtg ctgcgtatac cggatgaacc gtcccgtcgc tggtttgaca
    14401 gggatattct gcgggaggtt gttctggagg cacgggatga aatccgcagc atggcagatg
    14461 cactggcgaa aatccggaaa tgatgacgct ggcggggaaa aatctccgcc ttttcctgcc
    14521 ggtccccgga cagagcaaaa ggatgtcgcc gcaggctccg ctttacccgg ccatgcgggg
    14581 catggccttg tgggttttca gttatgtggc ctcagcgtcg tgtgcgggct gtgccgtgcc
    14641 tccatcttag ccggctggcg gggatgcaag ggtaaaatgc acgcggcggt cacccggtcc
    14701 ctccttcccg tctgccgtga ttttccggtc gttcacccgc tcagatttcg cggtctcacc
    14761 ctccaactcc cgtcagccgt gtgcgtcagg ctgcggcttc ccttgcgccc ttgcatcccc
    14821 gccttatcgc ccggcttttc tggaggcacg gcaccgcccg gtgtcgcgga atatgtgaac
    14881 aacggaggaa atcccatgca gtatgcgaaa cctgtcactc tgaacgttga agagtgcgac
    14941 cgcctttcct ttctgcctta cctgtttggc aacgattttc tgtatgccga ggcgtatgtg
    15001 tacgcgctgg cgcaaaaaat gatgccggaa tatcagggag gcttctggca tttcatccgc
    15061 ctgccggatg gtggcggtta catgatgccg gatggcgacc gttttcatct ggtcaacggt
    15121 gaaaactggt ttgaccgcac tgtgagtgct gatgccgcag gtatcatcct gacttccctt
    15181 gtgattaacc gccagttgtg gctgtaccac gacagcgggg atgcgggact gacacacctt
    15241 taccggatgc gtgatgcgca gttgtggagc cacattgaat ttcatcccga atgtaacgcg
    15301 atttacgcag cactggactg attaacggac ggggcggcga tgccgcccct gtaaaggaga
    15361 cgagaacatg tactgtactg ttaaagaaat tatccgcgaa gtgctgaata cagacgtgcc
    15421 ggacagtgaa tgtgtttttg ccgtggtgct gacccgcggg gatgtgcgcc acatagcccg
    15481 ggactggaac ctgtcagacg atgagctgga aaccgtcatg cagcggctgg acgatgcttt
    15541 tgtatatggc gcatgtgaca gagttgtttc cgatattgtc aatgaactga tggaggaaaa
    15601 gcgcgtaaac cgtcttgtaa cagtccctgc ggtgctgctg gaaaaagtga tggtgatggc
    15661 tggtagtgaa atttatcgcc tccatgctgt agggagcgag aacgggggcg acggtgacgc
    15721 atttgtcagg gaagaacgtg agattatgcg ggtaatgcgt caggcgctgg acggggagaa
    15781 cgggtaatga atatcagcac ggaaacccgc gaaattctgc gtaattacaa agctgtgatt
    15841 aatgcgcggc gtcgtgaaat ggggcagaaa ccgctcacca ccgcgcagat tgttgatgaa
    15901 atctgtgatt ttgtggcgaa tcagcaggcg gttttcctcg gtggtcacta tatccttcag
    15961 ggcagcagaa acaggtgata cagggcaggc ggtggaggcc gcctgtcagc ccgcgcgaaa
    16021 acccctggca ggctggcgcc tgccggaagg gaagcgggcc acgctggcgc gtcgcccgtt
    16081 acccttcctg ttggtgacag gcgttgtttc aggaagtacc tgttacagac ctgtcgttaa
    16141 agaaccttct tacacatgcg ccccttcggc tcccggctgc ctggcggcat ccggtcgtcg
    16201 gcccgactac gacaggcagc tgctggcgca cccgccagtc tccgccgaac ccgctggcgc
    16261 ggtttcgggc tgtcgctacg gtccgatgcg tgaaaacccg ggcttgcagc gaccggaacg
    16321 gtcgcgccgc ctttgtcgcg ccgtggcctg cgctcctggc atcccggcaa aaggccgccg
    16381 ggatgtaagg aaaccgcgcc tgatggcgcg gcagtctcct cttctgccgc cacctctgcg
    16441 gcctgtttcc cgctgtggac tttccggggg gcagacgtaa ccacctcccg ttccatcagt
    16501 ccaggggaag atataccggc cacaaaattt ttagccatga acccggcaaa aaattttgcg
    16561 tccgcccccg gacagctgtc ccgtgaggcg gtaaacggg
//
LOCUS       DAZKGF010000112        16581 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZKGF010000112 DAZKGF010000000
VERSION     DAZKGF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.112"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            53..1042
                     /gene="ldhA"
                     /locus_tag="ACHOQV_003376"
     CDS             53..1042
                     /gene="ldhA"
                     /locus_tag="ACHOQV_003376"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP9631691.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFEQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1153..1575
                     /gene="hslJ"
                     /locus_tag="ACHOQV_003377"
     CDS             1153..1575
                     /gene="hslJ"
                     /locus_tag="ACHOQV_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFP9631692.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1572..1838)
                     /gene="ydbJ"
                     /locus_tag="ACHOQV_003378"
     CDS             complement(1572..1838)
                     /gene="ydbJ"
                     /locus_tag="ACHOQV_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFP9631693.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTTIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2112..5636
                     /gene="nifJ"
                     /locus_tag="ACHOQV_003379"
     CDS             2112..5636
                     /gene="nifJ"
                     /locus_tag="ACHOQV_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP9631694.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPDSVRTVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSVDEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLKDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            6002..7135
                     /gene="ompN"
                     /locus_tag="ACHOQV_003380"
     CDS             6002..7135
                     /gene="ompN"
                     /locus_tag="ACHOQV_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFP9631695.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            7276..7710
                     /gene="uspF"
                     /locus_tag="ACHOQV_003381"
     CDS             7276..7710
                     /gene="uspF"
                     /locus_tag="ACHOQV_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFP9631696.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            7886..8821
                     /gene="ttcA"
                     /locus_tag="ACHOQV_003382"
     CDS             7886..8821
                     /gene="ttcA"
                     /locus_tag="ACHOQV_003382"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFP9631697.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(8950..10323)
                     /gene="dbpA"
                     /locus_tag="ACHOQV_003383"
     CDS             complement(8950..10323)
                     /gene="dbpA"
                     /locus_tag="ACHOQV_003383"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFP9631698.1"
                     /translation="MSAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPTILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRMLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPVNSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(10353..10526)
                     /gene="ynaL"
                     /locus_tag="ACHOQV_003384"
     CDS             complement(10353..10526)
                     /gene="ynaL"
                     /locus_tag="ACHOQV_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFP9631699.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(10801..11784)
                     /gene="zntB"
                     /locus_tag="ACHOQV_003385"
     CDS             complement(10801..11784)
                     /gene="zntB"
                     /locus_tag="ACHOQV_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFP9631700.1"
                     /translation="MEAIKGADVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(11798..11845)
                     /gene="ydaN"
                     /locus_tag="ACHOQV_003386"
     CDS             complement(11798..11845)
                     /gene="ydaN"
                     /locus_tag="ACHOQV_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFP9631701.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            12039..13271
                     /gene="dgcM"
                     /locus_tag="ACHOQV_003387"
     CDS             12039..13271
                     /gene="dgcM"
                     /locus_tag="ACHOQV_003387"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFP9631702.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(13292..13855)
                     /gene="smrA"
                     /locus_tag="ACHOQV_003388"
     CDS             complement(13292..13855)
                     /gene="smrA"
                     /locus_tag="ACHOQV_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFP9631703.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLTQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(14050..15564)
                     /locus_tag="ACHOQV_003389"
     CDS             complement(14050..15564)
                     /locus_tag="ACHOQV_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFP9631704.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGSAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VVIFSTVYLILVFLGQVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
     gene            complement(15561..>16581)
                     /locus_tag="ACHOQV_003390"
     CDS             complement(15561..>16581)
                     /locus_tag="ACHOQV_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9631705.1"
                     /translation="SKKQLIGVVAIGIILAGVVFFIWWVSKGRFIQTTDDAYIGGNIT
                     TVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSRKANLESIQA
                     TITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLVQSAAISQQIIDNARFDYQQVA
                     AKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPIDGIV
                     ANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKGEVF
                     HGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTV
                     SVDER"
BASE COUNT         4387 a         4185 c         3923 g         4086 t
ORIGIN      
        1 taaaatattt ttagtagctt aaatgtgatt caacatcact ggagaaagtc ttatgaaact
       61 cgccgtttat agcacaaaac agtacgacaa gaagtacctg caacaggtga acgagtcctt
      121 tggctttgag ctggaatttt ttgactttct gctgacggaa aaaaccgcta aaaccgccaa
      181 tggctgcgaa gcggtatgta ttttcgtaaa cgatgacggc agccgcccgg tgctggaaga
      241 gctgaaaaag cacggcgtta aatatatcgc cctgcgctgt gccggtttca ataacgtcga
      301 ccttgacgcg gcaaaagaac tggggctgaa agtagtccgt gttccagcct atgatccaga
      361 agccgttgct gaacacgcca tcggtatgat gatgacgctg aaccgccgta ttcaccgcgc
      421 gtatcagcgt acccgtgacg ccaacttctc tctggaaggt ctgaccggct ttactatgta
      481 tggcaaaacg gcaggcgtta tcggtaccgg caaaatcggt gtggcgatgc tgcgcattct
      541 gaaaggtttt ggtatgcgtc tgctggcgtt cgatccgtat ccaagtgcgg cggcgctgga
      601 actcggtgtg gagtatgtcg atctgccaac cctgttctct gaatcagacg ttatctctct
      661 gcactgcccg ctgacaccgg aaaactatca tctgttgaac gaagctgcct tcgaacagat
      721 gaaaaatggc gtgatgatcg tcaataccag tcgcggtgca ttgattgatt ctcaggcagc
      781 aattgaagcg ctgaaaaatc agaaaattgg ttcgttgggt atggacgtgt atgagaacga
      841 acgcgatctg ttctttgaag ataaatccaa cgacgtaatt caggatgacg tattccgtcg
      901 cctgtctgcc tgccacaacg tgctgtttac cgggcatcag gcattcctga cagcagaagc
      961 gctgaccagt atttctcaga ctacgctgca aaacttaagc aatctggaaa aaggcgaaac
     1021 ctgcccgaac gaactggttt aatcttgccg ctcccctgca ttccagggga gctgattcag
     1081 ataatcccca atgacctttc atcctctatt cttaaaatag tcctgagtca gaaactgtaa
     1141 ttgagaacca caatgaagaa agtagccgcg ctcgttgcgc taagcctgct gatggcggga
     1201 tgtgtaagta atgacaaaat tgctgtaacc ccagaacagc tacagcatca tcgctttgtg
     1261 ctggaaagcg taaacggtaa gcccgtgacc agcgataaaa atccgccaga aatcagcttt
     1321 ggtgaaaaga tgatgatttc cggcagcatg tgtaaccgct ttagcggtga aggcaaactg
     1381 tctaatggtg aactgaccgc caaagggctg gcaatgaccc gtatgatgtg cgctaacccg
     1441 cagcttaatg aactcgataa caccattagc gaaatgctga aagaaggtgc acaagtggat
     1501 ctgaccgcga accagttaac gctggcgacc gcgaaacaga cattaactta taagctggcg
     1561 gatttaatga attaatagct gccacagctc ccggcggcaa gtgactgttc gctacagcgt
     1621 ttgccgttgg gtaatgcaca catcccaatc gtcgtaccat ccagttgacg ggcaacagaa
     1681 agcgaaccgc cgatcattgc acaatttgct tctccactac tggacatcga cgcttttaaa
     1741 cctggcgcta cgtgcgccgc agtcgcctgc tgaacaggtt cactactaca cgccgacaac
     1801 aataaagcgg cacaccctac ccaaaacgct gctcgcatct tttcttcctc tgatcttcaa
     1861 gccaaacgac accgccataa ataataggca gcacagaggg cggcgtcgag agctgtcctg
     1921 agcgttgccc cgcattttta cttttttatg gctatttttt tgccctctgt ttgatcaaaa
     1981 cattcattac gctgatgtgg gggacacaaa agcgaaaatg cagaagaaag ccatttgcta
     2041 aaattgaagg attactactg ggcgcgcagc aatttcgtgc gcccctcatt tgcgcaatgt
     2101 aagggtgtca tatgattact attgacggta atggcgcggt tgcttcggtg gcgtttcgca
     2161 ccagtgaagt tatcgccatc taccctatta cccccagttc cacgatggca gaacaggctg
     2221 atgcctgggc cggaaacggc ttgaagaacg tttggggaga cacaccacgc gtggttgaaa
     2281 tgcagtcgga agcgggtgct atcgctaccg tgcatggcgc tttgcagacg ggtgccctct
     2341 caacatcgtt tacgtcatcg cagggtttgc tgctgatgat cccgacgctg tacaaactgg
     2401 caggcgaact gacgccattt gtcctgcatg tagcggcacg taccgttgcc acacatgcac
     2461 tctctatttt tggcgatcat tccgacgtta tggcggtgcg ccagacgggt tgcgcgatgt
     2521 tgtgtgcggc taacgtccag gaagcgcaag actttgctct catttcgcac atcgcaacgc
     2581 tgaagagccg cgtgccattt attcatttct ttgatggttt ccgcacgtcc cacgaaatca
     2641 ataaaattgt cccgctggcc gatgacacta ttcttgatct catgccgcag gccgaaattg
     2701 atgctcatcg cgcccgggcg ctcaacccgg aacatccggt gatccgcggt acgtccgcca
     2761 atcctgacac ttatttccag tctcgcgaag ccaccaaccc atggtacaac gcagtctatg
     2821 accatgttga acaggcgatg aatgatttcg ctgccgcgac aggtcgccag tatcagccgt
     2881 ttgagtatta cgggcatcca caagcggaac gggtgattat cctgatgggc tctgccattg
     2941 gcacctgtga agaagtggtt gatgaattgc tgacccgtgg cgaaaaagtc ggcgtgctga
     3001 aagttcgcct gtaccgcccc ttctccgcca aacacttact gcaagcgttg ccggactctg
     3061 ttcggacagt ggcggtgctg gatcgcacca aagagccagg tgcgcaagcg gaacccctgt
     3121 acctggatgt gatgaccgca ctggcagaag cctttaataa tggcgagcgt gaaaccctgc
     3181 cccgtgtcat tggtgggcgc tatggtcttt catccaaaga atttggcccg gactgtgtac
     3241 tggcggtatt tgccgagctc aacgcggcta aaccgaaagc gcgctttacg gtaggtattt
     3301 acgatgatgt gaccaatctg tcactgccgt tgccggaaaa caccctgcca aactcggcga
     3361 aacttgaagc cttgttttat ggccttggta gtgatggcag cgtttccgcg accaaaaaca
     3421 atatcaagat tatcggtaat tccacgccgt ggtacgcaca gggctatttt gtttacgact
     3481 ccaaaaaggc gggcggcctg acggtttctc accttcgcgt gagcgagcag ccgattcgtt
     3541 ccgcttatct gatttcccag gctgattttg ttggctgcca ccagttgcag tttatcgata
     3601 aatatcagat ggctgaacgt ttaaaacctg gcggcatttt cctgctcaac acgccgtaca
     3661 gcgtagatga agtatggtcg cgcttgccgc aggaagttca ggccgtgtta aaccagaaaa
     3721 aagcgcgctt ctatgtgatt aacgcggcga aaatcgcccg cgaatgtggt ctggcggccc
     3781 gtattaatac cgtcatgcag atggcttttt tccatctgac gcaaattctg cctggcgata
     3841 gcgccctcgc agaattgcag ggtgcgattg ccaaaagtta cagtagcaaa ggtcaggatc
     3901 tggtggagcg caactggcag gctctggcgc tggcgcgtga atccgtagaa gaagttccgt
     3961 tgcagccggt taatccgcac agcgccaatc gaccgcctgt ggtttccgat gccgctcctg
     4021 atttcgtgaa aaccgttacc gcggcgatgc tcgccgggct tggtgatgcc ctccccgttt
     4081 cggcgctgcc gccagacggc acctggccga tgggcaccac gcgctgggaa aaacgcaata
     4141 tcgccgaaga gatccccatc tggaaagagg aactctgtac ccaatgtaac cactgcgtcg
     4201 ctgcttgccc acattcggct attcgcgcaa aagtggtacc gcctgaagcg atggaaaacg
     4261 cccctgccag cctgcattcg ctggatgtga aatcgcgtga tatgcgcggg cagaaatatg
     4321 tcttgcaggt ggcaccggaa gattgcaccg gctgtaacct gtgcgtcgaa gtttgcccgg
     4381 cgaaagaccg tcagaatcca gagattaaag ccatcaatat gatgtcgcgc ctggaacatg
     4441 tcgaagaaga gaaaatcaat tacgatttct tcctcaacct cccagaaatc gaccgtagca
     4501 aactggaacg tattgatatt cgtacatcac agctgattac accgctgttt gaatattcag
     4561 gtgcttgctc cggttgtggc gagacgccgt atattaaatt gctgactcag ctctatggcg
     4621 accgaatgtt gatcgcgaac gccaccggct gttcttcaat ttatggcggt aacctgccct
     4681 ctacaccgta tactaccgat gccaacggtc gtggaccggc atgggcgaac tcgctgtttg
     4741 aagataacgc cgaatttggc cttggtttcc gcctgacggt cgatcaacac cgtgtccgcg
     4801 tgttgcgtct gctggatcaa tttgccgata aaatcccggc ggaattacta actgcgttga
     4861 aatcagacgc cacgccagag gttcgtcgtg aacaggttgc agctttacgc cagcaactca
     4921 aagatgttgc cgaagcacat gaactgctac gtgacgcaga tgcactggta gaaaaatcaa
     4981 tctggctgat tggtggtgat ggctgggctt acgatatcgg ctttggcggt ctggatcatg
     5041 tattgagttt gacggaaaac gtcaacattc tggtgctgga tacgcaatgc tattccaaca
     5101 ccggtggtca ggcgtcgaaa gcgacaccgc tgggtgcagt aactaagttc ggcgagcacg
     5161 gcaaacgtaa agcgcgtaaa gatcttggcg tcagtatgat gatgtacggt catgtttatg
     5221 tggcgcagat ttctctcggc gcgcagctca accagacggt gaaagcgatt caggaagcgg
     5281 aagcgtatcc ggggccatcg ctgatcatcg cttatagccc ctgtgaagag catggttacg
     5341 atctggcact cagtcacgac cagatgcgcc aactcactgc caccggcttc tggccgctat
     5401 atcgcttcga tccacgtcga gccgatgaag gcaaactgcc gctggcattg gattcacgcc
     5461 cgccgtcaga agcactggaa gaaacgttac ttcacgagca gcgtttccgt cgtctgaatt
     5521 cgcagcagcc agaagtggca gaacagttat ggaaagatgc tgcagctgat ttgcaaaaac
     5581 gctatgactt cctggcacaa atggccggaa aagcggaaaa aagcaacacc gattaaatgc
     5641 actggataag gattatccaa ttctaaaaaa aagcccggac gactgttcgg gcttgtcttt
     5701 ttatatgttg gaaaatcagt agcaatgcaa tggcccaaca gaaaataaaa tctgtgacaa
     5761 taaaggcata taacccgcgc agaataacgt atagcaaatt aaaagtataa tttttatttt
     5821 gtatgtatta tcttcaatac attcaccaga cttattatca tttcagaaga attatttgat
     5881 taaggtttta cttaaggcgt aacaaatgat atttatcggc taactgaact tctcctttag
     5941 gatgctttca ctccccaaaa tggggataaa aggcaaataa aataactaaa ggatttattc
     6001 aatgaaaagc aaagtactgg cacttttaat tcctgccctg ctcgccgcag gtgctgcaca
     6061 tgcagccgaa gtttataata aagacggcaa caaattagat ctgtatggca aagttgatgg
     6121 cctgcattat ttttctgata attcagcgaa agatggcgac cagagctatg cgcgtctggg
     6181 ttttaaaggc gaaacccaaa ttaacgatca actcactggc tacggtcaat gggaatacaa
     6241 tattcaggca aacaacactg aatcttcaac aaaccagtca tggacccgtc tggcatttgc
     6301 cggactgaaa tttgcagatt acggttcttt cgattacgga cgtaattatg gcgtaatgta
     6361 cgacatcgaa ggctggaccg atatgctgcc tgaatttggc ggtgactctt ataccaatgc
     6421 agacaacttt atgactggtc gtgccaacgg cgtcgcgact tatcgtaata ctgatttctt
     6481 cggtctggta aatggtctga acttcgcggt gcagtatcaa ggtaacaacg aaggagccag
     6541 taatggtcag gaaggcacca acaacggacg tgatgttcgc catgaaaacg gtgacggctg
     6601 gggtctttcc acaacatatg atttaggcat gggctttagc gctggtgcgg catacacctc
     6661 ttctgaccgc accaatgacc aggttaacca tactgcggcg ggtggtgata aagcagacgc
     6721 gtggactgct gggctaaaat acgatgctaa caatatttac ctggcaacca tgtattcaga
     6781 aacgcgtaac atgaccccgt ttggcgacag cgattatgca gttgcgaaca aaacccagaa
     6841 ctttgaagtt actgcacagt accagtttga ttttggtctg cgtccggcag tctctttcct
     6901 gatgtctaaa ggccgtgacc tgcacgctgc cggtggtgca gacaacccgg caggtgttga
     6961 tgataaagat ctggttaaat acgccgatgt tggcgcgact tactatttca acaaaaatat
     7021 gtccacctac gttgactata aaataaacct gttggatgaa gatgacagct tctacgctgc
     7081 caacggcatc tctaccgatg atattgtcgc tttaggtctg gtttatcagt tctaaatcct
     7141 cctgcccgct gttatggcgg gctttttctg cttattcttc ctcctttgat ctaaattaaa
     7201 aatgtgagct ccgtcattac acaaaaagtg tcatctggcg ttacacttta tgcggatact
     7261 aaaacaggag gttttatgaa cagaacgatt cttgtcccta tcgatatttc cgattcagaa
     7321 ttaactcaac gcgtgattag ccacgttgag gcagaggcaa agattgatga tgcagaggtt
     7381 catttcctga cggtaatacc ttcactgccc tactatgcct ctctgggttt agcgtattcc
     7441 gcagaattac cggcaatgga tgacctgaaa gcggaagcca aatcgcaact ggaagagatc
     7501 attaaaaaat ttaaactgcc aaccgacaga gtgcatgtcc atgttgagga aggctcgccc
     7561 aaagaccgca ttctggaatt ggcgaagaag atccccgctc atatgatcat cattgcttcc
     7621 catcgaccgg atatcaccac ttatctgctc ggttctaacg ccgcagcggt agtgcgtcac
     7681 gcagagtgct ccgtgctggt tgtgcgctga cattaacgcc cgcacattgc tgcgggcttt
     7741 ttgattcatt tcgcaaatgt gctgacattt tcccctctaa tccgtaccat acacgccaca
     7801 gtttttatat cagatttctt atgctgggcg ttccggcatg acgcatactc ttctgatgcc
     7861 atataacgaa ttgagtcgct tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca
     7921 atacaacctg aacaaattac aaaaacgtct gcgtcgtaac gtgggcgaag ccattgctga
     7981 cttcaatatg attgaagaag gcgatcgcat catggtttgc ctctccgggg gtaaagacag
     8041 ctataccatg ctggaaattc tgcgcaattt gcagcaaagc gcgccaatca atttttcgct
     8101 ggtggctgtt aacctcgatc aaaagcaacc gggcttcccg gaacacgttc tgcccgagta
     8161 tcttgaaacg ctgggcgttg agtacaagat tgtggaagag aacacttacg gtatcgtgaa
     8221 agagaagatt ccggagggca aaaccacttg ctcactgtgt tctcgtcttc gtcgcggtat
     8281 cctttatcgc accgcaacgg aactgggggc gacgaagatc gcgctgggtc accatcgtga
     8341 cgatatcctg caaacgttgt tcttaaatat gttctacggc ggcaagatga aaggtatgcc
     8401 tccgaaactg atgagcgatg atggcaaaca tattgtgatt cgtccgctgg cctactgccg
     8461 cgagaaagat attcagcgct ttgccgatgc aaaagcgttc ccgattattc cgtgcaacct
     8521 gtgcggttca cagcctaacc tgcaacgtca ggtgattgct gacatgttgc gtgactggga
     8581 taaacgttat ccagggcgta tcgagacgat gttcagcgcg atgcagaatg tggtgccgtc
     8641 gcatctgtgc gataccaacc tgttcgattt caaaggtatt acccacggtt ctgaagtggt
     8701 taacgggggt gatctggcgt ttgatcgcga agagatccct ctacaaccgg cgggttggca
     8761 gcctgaagaa gatgaaaatc agttggatga gttacggctg aatgtggttg aagtgaaata
     8821 acccggatag cgcccgatgc gcaagcttac cgggctactc ttatggaggc cggataagac
     8881 gcggccagcg tcgcatccgg caatcccgaa gaagatgttt actcttgcac cctgcgattc
     8941 aacatttcat tattttaata accgcacccg gcacgttttt cctttaatct tcccgccctg
     9001 taactgtttc catgctttat gagcaacagc ctgacgcacc gcgacataga catgcgccgg
     9061 atgcacggcg attttgccaa tatctgcgcc atcaagcccg atatctcctg tcagcgcacc
     9121 taatacatca cccgggcgca ttttggcttt tttcccgcca tcgatacata acgttgccat
     9181 ttctgcttcc agcggcacaa tggaactatt aactggcggc gtttgccagt taagttttat
     9241 ctgcaacatg tcagaaatga tattggcccg ctgtgcttct tccggggcac agaaactgat
     9301 cgccagaccg ctatttcctg cacgagctgt acgaccgatg cgatgtacat gaacttcagg
     9361 gtcccacgcc agctcaaagt tcaccaccag ctcaagcgat tttatatcca gaccacgcgc
     9421 agcaacatca gtcgcgacca gtacacgggc gctaccgtta gcaaaacgta ccagggtctg
     9481 atcgcgatcg cgttgctcca gatcgccgtg taacgacaat gcactttgcc ctacttcatt
     9541 cagcgcgtcg cagacagact ggcaatcttt tttggtattg caaaacacca cgcaagagga
     9601 tggctgatgc aagcttaata accgttgcaa cagaggaatt ttgcctttgc tggatgtctc
     9661 ataaaattgt tgttcaatag gtggcagagc atctgttgag tcaatttcaa tcgctaaagg
     9721 atcgcgttgc actcttccgc tgattgcggc gatggcttcc ggccaggttg ccgaaaacag
     9781 aagcgtctgt cgagatgctg gcgcaaaacg gatgacatca tcaatggcat cgctaaatcc
     9841 catatccagc atgcggtcgg cctcatccat caccagcgta ttcaacgcat ccagtgatac
     9901 cgtgcctttt tgcagatgat ccagcatacg gcccggcgtt gccacgataa tatgcggcgc
     9961 atgttgcaac gaatcacgct gcataccgaa cggttgacca ccgcacaacg tcaaaatttt
    10021 ggtatttggc agaaaacgcg ccagccgacg caattcacct gccacctgat ccgccagttc
    10081 acgcgtagga cacagcacta aagcctgggt ttgaaatagc gacgcatcaa tttgctgtaa
    10141 caagccgagg ccaaaagccg ccgttttgcc gctgccggtt ttcgcctgca cgcgaacatc
    10201 ttttccggca aggatcgtcg gaagcgcggc ggcctgcacc ggcgtcatgg ttaaataacc
    10261 caactcatta aggttcgtga gttgggcggg aggcaaaaca ttcagggtag aaaaagcgga
    10321 cacaatttat tctcgtggtc atcgacgcaa agtcagcagg cgcgtatcct cgcagatcta
    10381 cgctcacgat gcgacaattt aatcggttct tcgtcgggtg gtgggtcagg catgggttgc
    10441 gggcgaggga tcggatcggg cactggaaca ggatcgccag gaatcggttc agggacagga
    10501 atttgcaaat aaataagtgt cgtcatattt ccctccggtc attgggtgga ctcttaaagg
    10561 gtagacgctg acaaataaca ggcaaaaaaa agccgactca tcaaagtcgg cgtcgtacga
    10621 atcaattgtg ctatgcagta attcaaaaaa ggaagtaaga caatatggag cgcaacgccc
    10681 atcgcttgac gttgcattca cctgcaagag agatattgcc ctgaatgggt agagagttta
    10741 ttgacttcgc tcaaactttg cggcgttttt gtatacagac agccgggaaa attgcttttg
    10801 ttacaaccat ttactacgat gcaaccataa agcaacacca ccaataagaa caactaacag
    10861 aatacaaaaa attgaaaatc cgaattgcca cccgccgcct gggatcccac caaggttgac
    10921 gccaaataac cctgtcagaa aggtactggg taaaaagacc attgccatca acgacattgt
    10981 ataggtacga cgcgctaaat tttcctgcat cacctgagcg atttcatcag ccatcacgcc
    11041 agtccgtgct atacaggcgt cgatttcgtc aaggccgcgc ccaaggcgat cggcaatatc
    11101 ctgcatacga cggcgttggt catcgctcat ccacggcaaa cgttcactgg caagacgagc
    11161 ataaacatca cgttgcggtg ccatatagcg acgcatcaca attaattgtt tgcgcagcag
    11221 agccaggaat ccacgcggtg gaatttgctg atcaaggaga ttatcttcaa ggtcgataat
    11281 tttatcgtgc agctgctcga taaattcgct ggaatgatcg gtcaacgcat cacacacatc
    11341 caccagccat cccccgcaat cggtcggacc ggtgccttct tccagatcgc ttaccacatc
    11401 gtccagcgcc agcactttgc gttgtcgggt cgaaacaatt aaccgcccgt ccatatatac
    11461 acgcatggcg accagttgat cggggcgttc atcggtgctg ccgtttatac agcgcaatgt
    11521 aatcagcgta ccttcaccga gacggctgac tcggggccgt gtgctctcgc ccgccagcgc
    11581 atcacgtacg ttattgggaa gcagcggtgt tgtcgccagc cattgggcgc tatcatggtg
    11641 tacataatta aggtggagcc agcaggggtg cgcttcatca atcacatctg tattttccag
    11701 cggtttaacg ccgcctctac catccagcat ccaggcaaat actgcatccg ggacattaac
    11761 gtccgctccc ttaatcgctt ccacagtgcc tccatcatca acgcattatt ttgtagtcta
    11821 gccttctggc cctgttacgc aacatctcat caccccatta ccctgaaatg attaataaaa
    11881 ttctgtctaa attgaataaa aaaagcaaaa tgcttttcca tatacaaacc gtgtgaagtg
    11941 ttaaatagcg tctatcatta tcagaattat ctgatcatat gacgtggctt ttttgcgatc
    12001 ggatagcaac aaaaattgat aaaaataacg ggatctcaat gattacgcac aacttcaata
    12061 ccctggactt actcaccagt cctgtctgga tcgtttcgcc ctttgaggaa cagttaattt
    12121 atgccaacag cgcggcgcga ctgttgatgc aagacctcac gtttagtcag ctacgaaccg
    12181 gtccctattc cgtctcctca caaaaagaac tgccgaaata cctctccgat ctgcaaaacc
    12241 aacacgatat tatcgaaatc ctcaccgttc agcgcaatga agaggaaaca gcattgagct
    12301 gtcggcttgt tttgcgaaag ctgacagaag cagaaccggt gattattttc gaaggtatcg
    12361 aagcaccggc aacgctgggt ttaaaagcca gtcgctcggc aaattatcag cgcaaaaaac
    12421 aaggttttta tgcgcgcttt tttctgacta actctgcacc aatgttgttg attgacccct
    12481 cacgagatgg ccaaatcgtc gatgctaacc tcgccgcgct caatttctat ggctataacc
    12541 atgaaacgat gtgccagaaa catacctggg aaataaatat gctcgggcga cgcgtcatgc
    12601 ctatcatgca tgaaatttcg catttacccg gtggccataa gcctttgaat tttgttcata
    12661 aactggcgga tggttcgact cgtcatgtgc agacctatgc cggtccaatt gaaatttatg
    12721 gcgacaagct catgttatgt attgtgcatg atattactga gcaaaaacgg ctggaggagc
    12781 agctggaaca tgctgcccac catgatgcga tgaccggatt actgaatcgg cgacagtttt
    12841 atcacattac cgagccaggc caaatgcagc acctcgccat cgctcaggat tacagcttgt
    12901 tgctcatcga caccgatcgt tttaaacaca ttaacgatct ctatgggcat tctaaaggtg
    12961 atgaggtgtt atgcgccctt gcccgcactc tcgaaagttg cgctcgcaaa ggcgatttgg
    13021 tgtttcgttg gggaggcgaa gagtttgtct tattgctacc aagaacccca ctggataccg
    13081 cgctttcgct ggctgaaact atccgcgtaa gcgtggcaaa agtgagtatt tcgggcttac
    13141 cacgctttac tgtcagcatt ggtgtggcgc accacgaagg aaatgaaagc atcgatgaac
    13201 tgtttaaacg cgttgatgat gctttgtatc gagcgaaaaa tgatggacgc aaccgcgtgc
    13261 tggcggcata agccacggat gcgtctcgtg atcaacgact gcgcttagcg tggcgctccc
    13321 agttttcttg cttcgcctgc gctgttttac gtagtgcgac gtaacacgcc ccgctgccgc
    13381 catgatgcgg tagcgcagtg caatatgcct gaacatcatc aaattcggtc agccagcgcg
    13441 ccacatagct gcggacaata ttggcatgcg atttatcatc ccgtcctttg ccatgaataa
    13501 tcagcacgtt acgcaaacca tccgccaggg cttgttgaat gaaactgaac accattttgc
    13561 ggcactcttc caccggctgg cgcaaaagat tcaggctcgc ctgttgcgga tatttaccac
    13621 tgcgcagctt atccagcacc ccatgttgca acccttcccg ccgaaactcc agcggctgag
    13681 ttagcgggat gatgtcgaga aatccggtgg tgaggaaatt atcaagctgc agcgtgtcga
    13741 tacgctgcgg cgcacgttgg ttacgcgttg gatgccagtg gacatctgta gcacgtttca
    13801 gcggctggac atcttccatg gcgtcaagaa acagcgattt gtcgtcaagg ttcatgtaac
    13861 ctcctatgca ccttcacacg tcgtgattca gacgggttaa ttgttcatac ttactgcatc
    13921 gttcccttca cagattgaaa caatactcta cttcatacct gtgagtatct gcgcaggcgg
    13981 ggcattttat actagcctgt tagcagtatc gaacagttta gcccgagttt aattcagcac
    14041 aggctccgat caggatgatt tattcatagc aggaaccaac aaaaatgcga taaataacag
    14101 accactgatt agcagaaagg tatccgaaaa tgccatagtt tgcgcctccc tcagcatcaa
    14161 ctgactcaat aatttggttg aagccagtat ttccgatgtc tggtcacttc cactgcgatt
    14221 aaaaaacaaa gcacttctgg aaatgaagtc attcaccgta tggggaacag aaaccatttt
    14281 ttcacccatt ctcgaaaaat gaaaattagt tcgattattg agaatagagc cacacagggc
    14341 aataccaatc gcgcccccaa gattacgtgt taaattaaat acaccagagg ctaacttcag
    14401 ccgttccttt ggaattcccc ccagcgttaa tgttacgatg ggggccatcg caaactgctg
    14461 cgaaatccct ctaattgcct gtggaaacag taactcctgc catccccatt cgtgggttat
    14521 aggggtaaaa agatacattg aaaacacaaa tccacccagc cccgccatca acagccatcg
    14581 cagatttatt tttttactta accagaagta aaagggaaca gaaaataact ggaaaatacc
    14641 tgttgtgcaa acggcaaaac ctatctcttc ggcatttaat ccgcgaacct gccccaaaaa
    14701 cacaagtatt aaatatacag tagagaatat cacaacccct ccagaaaaag aaaaaaaaca
    14761 tcccagcgtg aaatttttat ccttaaaagc atgaagatcc attatcggat tggatattgt
    14821 aagtgtacgc gcagcaaata aaataaaact caccaatgcc agaactgatg tcagcaaaat
    14881 agtattatca tccagccatc cccagcgcgc tccttcttca agggtatatt ccagacatcc
    14941 cagtgtcatg gccaacagga taatactcgg ataatcagca actttcagta aagagagatc
    15001 aggtttatca aaattaacca gaaaaggaat actcaaaact aaatatatgc caggtaatat
    15061 attgatataa aatagccatc gccagtcgag gttatcagtg atccagcccc ccagagttgg
    15121 ccctagcgtc ggtgacagtg aggccagcgc actgactacc gcagcagccg accccaactc
    15181 tttaccctgg taataaataa acgccgtcgt gaaaactaaa gggatcatgg atgcgccagc
    15241 cgctccctgt aaagcacgaa acaaaatcat gatctgaata ttccatgcaa gtccacacgc
    15301 gatactcatt aaagtaaata ttcccgcgga tagagtaaaa agccaacgag tggaaaatac
    15361 ccgtgacagc cacccagaga gcggaataac aataatctca gcaattaaat agctggtctg
    15421 aagccaggcc atttcgtctt tacctgcgga cagaccacca cctatttcat ttagggatga
    15481 cgatacaatt tgaatatcaa gctgagcaat aaataatccc atacacatac tggcgaaagc
    15541 aatatgttta cgcatagaca tcatctctca tcgacactga cggtgacgga cagtcccggg
    15601 cgaagaagtt gctttaattc ctttgcgtcg tcaaatgcaa tccgtaccgg gacacgctga
    15661 acaattttgg tgaaattgcc agtggcattt tctatcggaa tcaagctgaa actggctcct
    15721 gtcgctgggg aaaggctttc aatgtgaccg tgaaacactt cccctttgag aatatcagca
    15781 cggatctcag ccttcattcc tggcttcatt cccgctattt ggttttcttt ataatttgca
    15841 tcaacccaca actcactgac tggtacaagt gaaactaacg atgtgccgcc ttccacccaa
    15901 ctgcctgtat gggcgctgcg gtttgcgaca atgccatcaa tcggcgcacg aacaagggta
    15961 tattccagat ccagtagtgc ctgagtcaaa gcggcctgaa cttcttcgat actcgcacgt
    16021 acgttttctt cctgagcaga taaaactgcc agccgttgct tctccaccag gaaatcgttc
    16081 gcggctttgc gctcctttgc tgcgacctgc tggtagtcaa acctggcgtt atcaataatt
    16141 tgctgcgata ttgccgctga ttgtacgagt ttttcataac gttcagtatc tcgcaggcgc
    16201 ttttgctcct catgttttac tgcctgccat gtttcactgg cactttgaat tatcgactgc
    16261 tgcatcgtaa tagtggcttg aatgctttcg agattggctt ttctactttt tatttttgcc
    16321 tcaagcctgg cgacatttgc acgataatcc cgatcatcga gacgcaggat aatatcgcct
    16381 ttttttacgc tctgattatc acgcacctct atagcagaaa tataaccaga aactttactt
    16441 gccaccgtgg taatattacc accaatgtaa gcatcatccg tcgtctgtat aaatcttcct
    16501 ttgcttaccc accagataaa aaagacaacg ccagccaaaa taataccgat cgctactacc
    16561 ccaataagct gttttttaga a
//
LOCUS       DAZKGF010000113        16501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZKGF010000113 DAZKGF010000000
VERSION     DAZKGF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.113"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            182..334
                     /gene="mokC"
                     /locus_tag="ACHOQV_003391"
     CDS             182..334
                     /gene="mokC"
                     /locus_tag="ACHOQV_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFP9631706.1"
                     /translation="MKQPKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(438..1568)
                     /gene="dnaJ"
                     /locus_tag="ACHOQV_003392"
     CDS             complement(438..1568)
                     /gene="dnaJ"
                     /locus_tag="ACHOQV_003392"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP9631707.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1657..3573)
                     /gene="dnaK"
                     /locus_tag="ACHOQV_003393"
     CDS             complement(1657..3573)
                     /gene="dnaK"
                     /locus_tag="ACHOQV_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP9631708.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEVGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3949..4353
                     /gene="yaaI"
                     /locus_tag="ACHOQV_003394"
     CDS             3949..4353
                     /gene="yaaI"
                     /locus_tag="ACHOQV_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFP9631709.1"
                     /translation="MKSVFTLSASLAISLLLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4379..5092
                     /gene="msyB"
                     /locus_tag="ACHOQV_003395"
     CDS             4379..5092
                     /gene="msyB"
                     /locus_tag="ACHOQV_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP9631710.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5241..5807
                     /gene="satP"
                     /locus_tag="ACHOQV_003396"
     CDS             5241..5807
                     /gene="satP"
                     /locus_tag="ACHOQV_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFP9631711.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5842..6429)
                     /gene="mog"
                     /locus_tag="ACHOQV_003397"
     CDS             complement(5842..6429)
                     /gene="mog"
                     /locus_tag="ACHOQV_003397"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFP9631712.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYIETAPEVVAAFRPKSARRDVSE"
     gene            complement(6544..7497)
                     /gene="tal"
                     /locus_tag="ACHOQV_003398"
     CDS             complement(6544..7497)
                     /gene="tal"
                     /locus_tag="ACHOQV_003398"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP9631713.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            7776..9206
                     /gene="yaaJ"
                     /locus_tag="ACHOQV_003399"
     CDS             7776..9206
                     /gene="yaaJ"
                     /locus_tag="ACHOQV_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFP9631714.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITTGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFALFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9276..10052
                     /gene="yaaA"
                     /locus_tag="ACHOQV_003400"
     CDS             9276..10052
                     /gene="yaaA"
                     /locus_tag="ACHOQV_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFP9631715.1"
                     /translation="MLILISPAKTLDYQSPLPTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10092..10388)
                     /locus_tag="ACHOQV_003401"
     CDS             complement(10092..10388)
                     /locus_tag="ACHOQV_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFP9631716.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEMTLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(10602..11888)
                     /gene="thrC"
                     /locus_tag="ACHOQV_003402"
     CDS             complement(10602..11888)
                     /gene="thrC"
                     /locus_tag="ACHOQV_003402"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFP9631717.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFVFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLHPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11889..12821)
                     /gene="thrB"
                     /locus_tag="ACHOQV_003403"
     CDS             complement(11889..12821)
                     /gene="thrB"
                     /locus_tag="ACHOQV_003403"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFP9631718.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12823..15285)
                     /gene="thrA"
                     /locus_tag="ACHOQV_003404"
     CDS             complement(12823..15285)
                     /gene="thrA"
                     /locus_tag="ACHOQV_003404"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFP9631719.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15311..15430)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15366..15431)
                     /gene="thrL"
                     /locus_tag="ACHOQV_003405"
     CDS             complement(15366..15431)
                     /gene="thrL"
                     /locus_tag="ACHOQV_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFP9631720.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15645..16331)
                     /gene="yjtD"
                     /locus_tag="ACHOQV_003406"
     CDS             complement(15645..16331)
                     /gene="yjtD"
                     /locus_tag="ACHOQV_003406"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP9631721.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADKHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3824 a         4415 c         4080 g         4182 t
ORIGIN      
        1 aatgggttaa agagagcgat ataatagcgg cgggtgcttg aggctgtctg tctcaggcat
       61 tagctgaacg gcagatagag aaaagccccg agtgatattt tactatcaac ccgaggcccc
      121 ctatatgcag aacacatgta gagtgcctct tactgaccgt aaggtcaagg agaagagagc
      181 aatgaagcag cctaaggcga tgattgtcgc cctgatcgtc atctgtatca ccgccgtagt
      241 ggcggcgctg gtaacgagaa aagacctctg tgaggttcac atccgaactg gccagacgga
      301 ggttgctgtt ttcacggctt acgaatccga gtaagagcaa cggcggggag tgatccccgc
      361 cacttttcag gtgctcgcat atcttcaacg caccctattt ttacccaggc ctgcccacgg
      421 gcaggctttt ggggagatta gcgggtcagg tcgtcaaaaa acttcttcac accatcaaag
      481 aagctctttg agcgcgggct gttgtgctcg ccggttgggc caccgaagct ttcttgcagc
      541 tcttgcagca gctgtttctg cttctcgttc aggcctaccg gtgtttcgac gacaacgcgg
      601 cacagcaaat caccctgtgc gccaccgcgg acagacttga cgcctttacc gcgcatacgg
      661 aacagcttac cggtctgggt ttcgccaggc actttcagtt tgacgcgacc atcaagggtc
      721 ggtacttcga tttcgccacc cagcgccgcc atagcgaagt tgatcgggac ttcgcaatag
      781 aggttgttgc cttcacgctc gaaaatcggg tgctgtttaa cctgaacctg aacgtacaga
      841 tcgcctgccg gtgcgccatg ctcgcccgct tcaccttcgc ccgcaagacg gatgcggtct
      901 ccagtgtcca cccctgccgg gattttaacg gacagcgttt tgctgcgctc aacacgacca
      961 tgaccatgac atttgttgca cggatctttg atcagcgtac cgcggccctg acagtgtgga
     1021 caggtctgct gcacggcaaa gaaaccctgg cgcatctgca cctgaccaga accatgacag
     1081 gtcggacagg tctgcggctg tgtacctggt tttgcaccgc taccgtggca aacgtcacac
     1141 tcttccagag tcggaatgcg gatctctttg gtcacgccac gtacagcttc ttcgagggtg
     1201 agctccatgt tatagcgtaa atcagcaccg cgcgccgcac gttgacgacc acgtccgccg
     1261 ccaaaaatat cgccgaaaac gtcaccaaaa atatcgctga agtctgcgcc gccgccgaaa
     1321 ccgccgcctc ccatgccacc ttgctcaaac gcagcatgac catactgatc gtatgccgca
     1381 cgtttttgcg agtcggtcag aacttcataa gcttccttga tctctttaaa tttcgcctcg
     1441 gcctctttgt caccctggtt acggtccggg tggtatttca tggccaggcg tttgtaggcc
     1501 tttttgattt cacgctcttc cgctgttttg gaaacgccta aaatctcgta ataatcttgc
     1561 ttagccatct tttttaaatt gcccctagat gaatgcacgg gcggagagga aattcccctt
     1621 cgcccgtgtc agtataatta cccgtttata gggcgattat tttttgtctt tgacttcttc
     1681 aaattcagcg tcgacaacat cgtcatcttt cgcgttgttt gcagaagcat cagcaccggc
     1741 agtctgctgc tgggcatgtt gctgctgggc gatttccatc agtttctggg aaacctgtgc
     1801 cagttcctgc attttcgctt cgatagcggc tttgtcttca cctttcagag cagtttccag
     1861 tgcagtcagt gcagactcga tagcagtttt gtcgtcagcc ggcagtttgt cgcccacttc
     1921 ttcaacctgc ttacgggtgc tgtgcagcag atggtcgccc tggttgcgag tctgtaccag
     1981 ctcttcaaac ttacggtcag cttcggcgtt agcttctgcg tcgcgtacca ttttctggat
     2041 ttcatcttcg ttcagaccag aagaagcctt gatggtgatc ttctgctctt taccgctgtt
     2101 tttatctttc gcggaaacgt gcaggatacc gtcagcatcg atatcgaagg taacttcgat
     2161 ctgcggcatg ccgcgcggtg ccgggttgat accatccagg ttgaactgac ccagagattt
     2221 gttatcagct gcacgtttac gttcaccctg cagcacatgg atggttaccg cagactggtt
     2281 gtcttcagcg gtagagaaca cctggctgtg cttggtcggg atagtggtgt ttttcgcgat
     2341 cagcgtcgtc atcacaccgc ccatggtttc gatacccaga gacagcgggg taacgtccag
     2401 cagcagtacg tcttttacgt caccagtcag aacaccaccc tgaacagcag caccgattgc
     2461 tacagcttcg tccgggttaa cgtctttacg cggctcttta ccaaagaact cagcaacttt
     2521 cttctgaacc attggcatac gagtctgacc accaacgagg ataacgtcgt cgatatcaga
     2581 tacggacagg ccagcgtcct gcagtgcaac tttcagcggc tcaatggaac ggtttaccag
     2641 atcttcaacc aggctttcca gtttcgcacg agtcactttg atgttcatgt gtttcggacc
     2701 ggtcgcgtct gcagtgatgt acggcaggtt aacgtcggtc tgctgagcgg aagacagttc
     2761 gattttcgct ttttctgccg cttctttcag gcgctgcatt gccagcggat cgttgcgcag
     2821 gtcaatgccc tgatctttct tgaattcttc aaccaggtag ttgatcagac ggctgtcgaa
     2881 gtcttcacca cccaggtggg tatcaccgtt ggttgccaga acttcgaagg ttttttcgcc
     2941 gtcaacttcg tcgatttcga taatagaaat atcgaaagta ccaccaccca ggtcataaac
     3001 cgcgatagta cggttgccag tacctttgtc cagaccgtaa gccagcgcag ctgcggtcgg
     3061 ttcgttgatg atacgtttta cttccagacc agcgatacgg cctgcgtctt tggttgcctg
     3121 acgctgagca tcgttaaagt atgccggtac ggtgataaca gcttcagtta ccggttcacc
     3181 caggtaatct tcagcggttt tcttcatttt tttcagcact tcagcagaga tctgcggcgg
     3241 tgccattttc tggcctttaa cttcgaccca tgcgtcgccg ttatcagcag caataatttt
     3301 gaacggcatg atggaaacat cacgctgtac ttcttcgtcc tggaagcggc ggccaatcag
     3361 gcgtttaatc gcaaacaggg tgttttgcgg gttcgtcact gcctgacgtt tagccggctg
     3421 accaaccaga gtttcaccat cctgggtata ggcaatgata gaaggcgtgg tgcgatcgcc
     3481 ttcggcgttc tccagtacac gaggagtggt gccatccata atcgctacac aagagttggt
     3541 agtacccagg tcgataccaa ttattttacc catctaaacg tctccactat atattcggtc
     3601 atcatgtggt tgtgagtctg taataggggc gaaacgtctg gtttcaactg cccaatttca
     3661 tttttttgca gactcactca ctgcggttga ctactaaatg gggtcgtaac ccacgtcatc
     3721 aagggggaga tgcaaaaatt ttttgtgctt ttgcgcagaa atttcgcgat atgccagaac
     3781 aagacatgaa actgacagca attttattca tatagagaat gaatagcaga caagacattc
     3841 atagaaaaat tgtaataaaa agaaaatatt acgtacctga atcttctctt tatcagcagt
     3901 aaactagtgg gtattcatcc cctacctctc cccactaaga gaatccttat gaaatccgtt
     3961 tttacgcttt ccgccagcct ggcgattagc ctgctgctgt gctgcacggc gcaggcaaac
     4021 gaccataaaa tcctcggcgt cattgcaatg ccacgtaacg aaaccaacga tctggcgctg
     4081 aaacttcctg tttgccgaat tgttaaacgc atacaactct cagccgacca tggcgattta
     4141 cagttaagcg gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca gaccctgaat
     4201 attccttcag aaataaaaga agggcaaacc actgactgga tcaacattaa cagcgataac
     4261 gacaataaac gctgcgtctc aaaaatcacc ttttctggtc atacggtgaa ctcatcggat
     4321 atggccacgc tgaaaattat cggcgacgac taacgccaga atatagaagc cacaaaaaat
     4381 gaatgttaat tacctgaatg attcagatct ggattttctc cagcattgta gtgaggaaca
     4441 actggcaaat ttcgcccggc tgctcaccca taatgaaaaa ggcaaaactc gcctctccag
     4501 cgtactgatg cgcaacgaac tgtttaaatc gatggaagga catcccgagc aacaccgccg
     4561 caactggcag ctgattgccg gagaattaca acattttggt ggcgatagta tcgccaacaa
     4621 actgcgcgga cacggtaaat tgtatcgggc cattttgctc gatgtttcaa aacgattgaa
     4681 gctgaaagcc gacaaagaga tgtctacgtt tgaaattgag caacagttac tggaacaatt
     4741 tctgcgtaat acctggaaga aaatggacga ggaacataag caggagtttc tgcacgcggt
     4801 cgatgccagg gtgaatgagc tggaagagct gctgccgctg ctgatgaaag acaaattatt
     4861 ggcaaaaggc gtgtcgcatt tgctttccag ccaattgacc cgcattttac gcacccacgc
     4921 agcaatgagc gtacttgggc atggtttgct tcgcggcgcg gggctgggag gccctgtagg
     4981 cgcggcacta aatggggtta aagcggtcag cggcagtgcc tatcgcgtga caattccagc
     5041 cgtactgcaa atcgcctgcc tgcgccggat ggttagtgcc actcaggtct gataccggaa
     5101 accggggaaa attttcattt tcccctcaaa agatcgtaga cactgcccca ctggctgatt
     5161 attatgccgc gccctgaaaa cactacagtt attcagggaa attatttcac cattcattcg
     5221 atgatgattt ttgaggaatt atgggcaaca ctaagttggc taatccggca ccgctgggcc
     5281 tgatgggctt cggcatgacc accattctgc ttaacctgca caacgtgggt tattttgctc
     5341 tggacggtat tattcttgcc atgggcattt tctacggcgg catcgcgcaa atttttgctg
     5401 gtctgctgga gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc tcttacggtt
     5461 ctttctggct gacgctggtt gcgattctgc tgatgccgaa actgggtctg accgatgcgc
     5521 caaatgcaca gttccttggt gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt
     5581 tcttcggcac gctgaaaggc gcacgcgttc tgcaattcgt tttctttagc ctgaccgtgc
     5641 tgtttgccct gctggcgatc ggtaacattg ccggtaacgc cgcaatcatc cactttgccg
     5701 gctggattgg tctgatctgc ggtgccagcg caatctatct ggcgatgggt gaagtactga
     5761 acgagcagtt tggtcgcacc gttctgccga ttggtgaatc ccattaattg ttttgagatc
     5821 cccccgctcg gggggatttt tttattcgct aacgtcgcgt cttgcactct tcggtctgaa
     5881 tgctgcaacc acttccggtg ccgtttcaat gtatggccct tccagcaact gaatgcagta
     5941 cggtacgctg gcaaaaatac catgcaccac aacgttaccc gcagcgtcct tcacgccttc
     6001 cagcgtctct ttaatcgact tcggctgacc gggtaagtta aggatcagcg cctgtttgcg
     6061 aatcacgccc acctgacgcg aaaggatcgc agttggtaca aaatgcaggc tgatctggcg
     6121 catctgttca ccaaagccag gcatctcgcg gtccgctact gccagcgtcg catcgggcgt
     6181 tacgtcacga cgcgccgggc cagttccgcc cgtggtgagc accagatggc aactcatttc
     6241 atccaccagc tcacacaacg tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt
     6301 ttccagttca aacggcgtgg ttagcgccga tgtcagccat tcttccagcg cagggatgcc
     6361 tttatcctga taaacgccgc tggatgcgcg atcagagatg gaaactaagc caatacgtaa
     6421 agtattcatc aagatttttc cgtttaaaca gtctcgttaa acagaatgat acactgcgaa
     6481 gggagtgaca gacaggcttc agaaaaggta gcgtgaccga cttcccggtc acgctaagaa
     6541 tgattacagc agatcgccga tcattttttc cagtttttcc tggtcaacag caaacttacg
     6601 gataccttcc gccagtttat ctactgccat tggatcctgg ttatgctgcc acaggaactc
     6661 ggactcagtg atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc
     6721 gatagcccct tcgctctcag ccagctcttt cagcagcgcc ggtgcgatgg tcagacggtc
     6781 gcagcctgcc agttccagaa tttcgccgat gttacggaag cttgcgccca tcaccacggt
     6841 ttcataaccg tgctctttgt agtactggta gatttcagat acagaaacca cgcccggatc
     6901 ttctgccgga gcgtactctt tcttgtcggt attcgctttg taccagtcaa gaatacggcc
     6961 aacaaacggc gagatcagga acacgcccgc ttccgcacaa gcacgagcct gagcgaagga
     7021 gaacagcagg gtcaggttac agttaatacc ttctttttcc agctgttctg cagcacggat
     7081 accctgccag gtagaagcca gtttgatcag aatacgatcg ttgctaatcc ctgcatcgtt
     7141 gtagagtttg atcaggcgtt ttgctttcgc aattgacgct tcggtgtcat aggaaagacg
     7201 cgcatcaact tcagttgaga tacggcccgg aaccagtttc aggatttcca gaccaatatt
     7261 tactgccagt ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt
     7321 cgcccaggcg acagcatcat caatcaactt acggtattcc ggaatctgcg ctgcgttaag
     7381 aatgagagaa gggttggttg tggcatcctg cggttgatac agcttcattg ccgcgatgtc
     7441 cccagtgtcg gccactacgg tggtgtactg acgaagggag gtcaatttgt ccgtcatgat
     7501 agtatttctc tttaaacagc ttgttagggg gatgtaaccg gtctgccctg atgatatcac
     7561 gacgcaacgt cagcgcaacc gccgacaaga cggttatcgc caggcgagac tgtttcggat
     7621 ttctgacact gcgttgatat cgcccgccat ttttatacaa aacctcatgt atgctacgca
     7681 gaagttatca agtacctcgt agcgtatata cttcttaaac aattggtaac gtttacacag
     7741 gaaagtcatc gcgaccggca ataagaggga tatgcatgcc agattttttc tcctttatta
     7801 acagcgtcct ttggggatcg gtaatgattt acctgctctt cggcgcaggt tgttggttca
     7861 cttttcgcac cggatttgtg cagtttcgct acatccgcca gtttggcaaa agtcttaaaa
     7921 atagcattca tccacagcca ggcggtttaa cctcatttca gtcattgtgt accagtcttg
     7981 cggcgcgcgt gggtagcggc aacctggctg gcgttgcgct ggctattacc accggtggac
     8041 caggtgccgt cttctggatg tgggttgccg cgtttatcgg catggcgacc tcgtttgccg
     8101 aatgttccct tgcacaactt tataaagaac gtgacgttaa tgggcagttt cgtggcggac
     8161 cggcatggta tatggcgcgc gggctgggga tgcgctggat gggcgttctg ttcgccctct
     8221 ttttgctcat cgcctacggc ataattttca gcggagttca ggcgaacgcc gttgcccgag
     8281 ccctgagttt ttcttttgat tttcccccgc tggtgacagg cattattctt gctgtctttg
     8341 ctctgctggc aatcactcgc ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc
     8401 cgttgatggc gataatctgg gtactgacca gcctggtaat atgcgtaatg aatatcgggc
     8461 aacttcccca cgtcatttgg tctatttttg agagtgcttt tggctggcag gaagcggcag
     8521 gcggcgcggc gggatatacc ttaagccagg cgattactaa cggttttcag cgcagtatgt
     8581 tttccaatga ggcgggaatg gggtcgacgc caaacgcggc agcggcggct gcgtcctggc
     8641 ctccgcatcc cgcagcgcaa ggaattgtcc agatgattgg catttttatc gacaccctgg
     8701 tcatctgtac ggcaagcgcc atgctgatat tactggcggg taacggcaca acctacatgc
     8761 cgctggaagg tattcagctt atccagaagg cgatgcgggt actcatgggt tcctggggtg
     8821 ctgagtttgt taccctcgtg gttattctgt ttgccttcag ctccatcgtt gccaactaca
     8881 tttatgccga aaacaatctc ttcttcttac gcctgaacaa ccctaaagcg atctggtgtc
     8941 tgcggatctg caccttcgca acggtcatcg gcggcacctt gctaagtctt ccgctgatgt
     9001 ggcaactggc agatatcata atggcctgca tggctattac caatttgacc gccatattac
     9061 tgctctcgcc tgtggttcat accattgcca gtgattatct acgccagcgt aaactcggcg
     9121 tgcgcccggt gtttgatccg ttgcgttatc cggatatcgg tcgccagctt tctccggacg
     9181 cgtgggatga tgtttcgcag gagtaatcac aactatcgat caactcattc tctttttttg
     9241 ctaaagtcgg cataaatttc ctgcaaggac tggatatgct gattcttatt tcacctgcga
     9301 aaacgctcga ttaccaaagc ccgttgccca ccacgcgcta tacgctgccg gagctgttag
     9361 acaattccca gcagttgatc catgaagcgc ggaaactgac gcctccgcag attagcacgc
     9421 tgatgcgcat cagcgacaaa ctggcgggta tcaacgccgc tcgctttcat gactggcagc
     9481 cagatttcac gccggagaat gcccgccagg cgattctggc attcaaaggt gatgtctaca
     9541 ccggcttgca ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa cagcatttgc
     9601 gaatgctttc cggcttgtat ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc
     9661 tggaaatggg gatccgtctt gagaatgccc gagggaaaga tctgtatcaa ttctggggag
     9721 atatcatcac caacaagctg aacgaggcgc tcgcagcaca aggcgataat gtggtgatta
     9781 atctggcgtc agatgagtat tttaaatcgg tgaagccgaa gaaattgaat gccgagatta
     9841 tcaagccggt gttcctcgat gagaagaacg gcaaatttaa gatcatcagc ttctacgcta
     9901 agaaagcacg cggtctgatg agtcgtttca ttattgaaaa tcggctgacc aaaccagagc
     9961 aactgactgg ttttaatagc gaaggttact tctttgatga agcttcctcc agcaacggcg
    10021 aactggtgtt taaacgctac gagcagcgtt aagtttgcag tcaatgccgg atggcaatcc
    10081 ggcattgata tttagcgatg atgtttgtca ggccccggac gatgatcgcc acgatgatca
    10141 ttgtgcttgt tatggcgcgg cgatggcggc ggtccatatg ggtgccagcg attgcctcgc
    10201 cattcataat gttgtttcca ccagccgtgg tcgcgccagt gaccgccatc ccagtaatag
    10261 ccacgattat cacgatcgcc tatctgtaat tttactgacg ggactaacgt catttccgca
    10321 gcctgcgcag ccatgggagc gaccagaacc agggaaagtg cgagtacgat agattgcatc
    10381 tttttcacag gttactcctt ttatcattgc ccgttatggg tcgatatccg gagattacgc
    10441 gatgagaacg gcagttgtta ttctccgcaa tcctaatctc taagttaccg catttattga
    10501 gcatttctcc tttttccctg tcgtttttct ccataatttc ttcataaaaa agccgggctg
    10561 cataaaagca aacccggcct gattgagata atgaatagat attactgatg attcatcatc
    10621 aatttacgca acgcagcaaa atcggcgggc agattatgtg aaagcaaggg taaatcggca
    10681 cgttctgcca gctcttttgg cagatccaac gtttcaccga gaatcgcttc cacgctctct
    10741 ttaaatttcg ccggatgcgc ggtgccgagg aacaagccat attcgcctgg atgcaactgg
    10801 tcacgcagtg cacgatacgc tacggcagcg tgcggctccg aagtgtagcc cagttctttt
    10861 aactcacgca ttgtctgttg cgtggtttca tcatccacgg ctgcataacc cagctctttc
    10921 agttgccaga ttttgcggcg gaacaactct tccacacgcg gccagttgtt cggctgactc
    10981 acgtccatcg cgttggataa cgtcgcctga gtcgctttgg gtgaccactg accgtcgtgc
    11041 aggaaacgtg gcacggtatc gttcacgttg gtcgcagcaa taaaacgttt caccggcaga
    11101 ccgagtgact tcgccagcag acccgccgtc aaatcgccga agtttccgct tggcaccgag
    11161 acaaccagct ggttgcgcgc ctcctgcggc agctgcgcaa cagcttcaaa gtagtagcaa
    11221 atctgcgcca gcaaacggct gatgttaatc gagttagctg agtttagccc cagcgccact
    11281 ttcagttctt catcatcaaa cgcctgcttc accagcgcct gacaggcatc gaaatcgccg
    11341 tcgatggcaa cagtttcaat attgccgccc aatgtacaga acagtttttc ttgcagtgga
    11401 ctgattttgc ctcgtggata gaggataacc actttcacat tcggcaaacc gtagaaagca
    11461 tgagccactg ccgctccggt atcaccggag gtcgcggtca gaatggtcac tggcttatcg
    11521 cccgcaatat gggtcagcat ttgtgccata aagcgaccgc cgaaatcttt aaatgccagc
    11581 gttggcccgt ggaacaattc cagacaaccg acatcgcttt caacattggc gaccggagcc
    11641 gggaagacaa acgccgcgcg tacgcgctct tccaggattt cctgcgggat ttcatcacca
    11701 ataaacgccg agaggatctt cgcactgcgg gtgacaaaat ccagcttcag catctcatca
    11761 atttcagtca ggctgaattc cggcaggtcg tgcgggaaaa acagcccctg atttttgccc
    11821 aacccctggg ttacggcttg cgcaaagctg acctgctcat tgtgatcttt cagattgtag
    11881 agtttcattt agttttccag tactcgtgcg cccgccgtat ccagccggca aatatgaaca
    11941 aaaccttcct gattttgcag gtagttctta cccaaccagt cggcaacgcg ctgggcggta
    12001 tccggcttgt cacacagagc gaacaaagtc gggccggagc cggagatacc gctcgctacc
    12061 gcgccgattt ccgcgaccgc ctgccgcgcc tgccggaagc ctggcagtaa ccgttcacgg
    12121 tagggttcag cgataacatc tttcatcagc ttcgcggcaa gctcaggctg acgggaatag
    12181 caggcgtgaa taaagcctgc cagatgtcgc ccgtgcgcaa tgcaatcctg gcggcgatac
    12241 tgcgccggta aaatagccct ggcttctgcc gtcgagactt taatccccgg atacgccagc
    12301 acccacagcc actcatcaaa ccctggcact tgctggctga tgatgtcgtt ttcttcgatc
    12361 atcaactgca taccaccgag aaaacacggt gccacgttgt cgtaatgaat gctgccggag
    12421 atacgccctt ccagctcgcc catcaaagcc agcaaacggg tgtcattaag cggcttgccg
    12481 cagtgttcat tcatcgccat cagcgccgcg accactgagc aggcgctgga gcctaagccc
    12541 gagccgatcg gcatattctt ttccagggtc atcgccactg gaatttgctt acccagctcc
    12601 tggcaaaaac gctcccaaca ctgataaacg atattttccc gaggttctga cggcagctta
    12661 tcggcaaagc gtccgaggtt attgagactg aatgtctctg ccgcctcaac cgtgactaca
    12721 tctccgagca atgcaccatc aacgggtgtc accgccgccc cgagcacatc gaacccgacg
    12781 ctcatattgg cactggaagc cggggcataa actttaacca tgtcagactc ctaacttcca
    12841 tgagagggta cgtagcagat cggcaaagac accggcagct gtaacgtcat tgcccgcacc
    12901 atatccgcgc agcaccaacg gcagcggctg ataatagtgg ctatagaagg ccagggcgtt
    12961 ttcgccgttt ttcactttga acagcggatc gttgccatcc acttcagcaa tcttcacgcg
    13021 gcagacgcca tcttcatcaa tattgccaac atagcgcaaa acttttcctt catcacgggc
    13081 cttcgccaca cgagcggcaa agagatcatc gagctgtgac agattcgcca taaaagcggc
    13141 aacatcaccc tcggcgttaa actcagcggg tagcacaggt tcaatttcaa tatccgccag
    13201 ctccagttca cgtcccgttt cacgagcgag aatcaatagt ttacgcgcca catccatacc
    13261 agaaagatca tctcgcggat ccggttcggt ataacccatt tcccgcgcca acgtggtcgc
    13321 ctcggagaaa ctcatgcctt cgtctaactt gccgaagata taagaaagcg aaccagaaag
    13381 aatgccggag aacttcatca attcatcacc tgcattgagc agattttgca ggttctcaat
    13441 aaccggtaat ccagccccaa cgttggtgtc atagaggaat ttacgccgcg atttttccgc
    13501 cgcatgacgc aacagatggt agtaatccat cgacgaggtg ttagcctttt tgttcggcgt
    13561 gacgacgtgg aaaccttcgc gcaggaagtc ggcatattga tccgccactg cctggctgga
    13621 ggtgcagtca acaatgaccg ggttcagcag atgatattct ttcacgaggc gaattaagcg
    13681 cccgagatta aacggctctt tggcttgcgc cagttcttcc tgccagtttt ccagattaag
    13741 gccatgtaca ttggtgagca gagccttcga gttggcaacg ccgcagacac gtaagtcgat
    13801 atgtttattc ttcaaccagc tttgctgacg cttcagttgc tccagcagcg caccgccaac
    13861 gccaccgacg ccaatcacaa acacttcgat aacctgatcg gtattgaaca gcatctgatg
    13921 agtaacgcgc acgccagtgg tcgcatcatc gttatttacc acgacagaga ttgagcgttc
    13981 agaagatccc tgagcaatgg cgacaatgtt gatattggcg cgggccagcg cggcaaagaa
    14041 tttcgccgag atcccacgca aggtgcgcat accatcacct accaccgaga taatggccag
    14101 ccgttccgtc accgccagcg gctccagtaa gccttctttc agttccaggt agaactcttc
    14161 ctgcattgcc cgttcagctc gcacacagtc gctttgtgga acgcagaaac tgatactgta
    14221 ttcggaagat gattgcgtaa tcagcaccac ggaaatacgg gcgcgtgaca tcgctgcaaa
    14281 gacgcgcgcc gccatgccga ccatcccttt catccccgga ccagaaacgc tgaacattgc
    14341 catgttattc agattggaaa tgcccttgac cggtaattcg tcttcatcac ggctggcacc
    14401 aatgagcgta cctggtgctt gaggatttcc ggtattttta atcaggcaag ggatctggaa
    14461 ctgggcgatg ggggtaattg tgcgggggtg aagaacttta gcgccgaagt aggaaagctc
    14521 catcgcttcc tggtaggaca tcgacttcaa caacctcgca tcgggcacct gacgcgggtc
    14581 gcaggtatag accccgtcaa cgtccgtcca aatctcgcaa caatcggcgc gtaaacaggc
    14641 agccagcacc gccgcggagt agtcggaacc gttacgtcca agcaccacca gttcgccttt
    14701 ttcattaccg gcggtgaaac ctgccatcaa caccatgtga tcagccggaa tacgacttgc
    14761 cgcaatacgg cgggtggact cagcaatatc gacggtagat tcgaggtaat gccccactgc
    14821 cagcagtttt tcgaccggat cgataacggt aacgttgtga ccacgcgctt ctaacacgcc
    14881 ggccataatg gcgatcgaca ttttctcgcc acggcaaatc agcgcagcgt tgatgctatc
    14941 cgggcactgc cccaacaaac taatgccatg caggacatgt tttatttggg caaattcctg
    15001 gtcgacgaaa gttttcagtt gcgccagcgg gaatcccggc tgagcggcgg cgagtcccgt
    15061 cagaagttcg gcaaaaatac gttcggcatc gctgatattg ggtaaagcat cctggccgct
    15121 aatggttttt tcaatcatcg ccaccaggtg gttggtgatt ttggcggggg cagagaggac
    15181 ggtggccacc tgcccctgcc tggcattgct ttccagaata tcggcaaccc gcagaaaacg
    15241 ttctgcattt gccactgatg taccgccgaa cttcaacact cgcatggttg ttacctcgtt
    15301 acctttggtc gaaaaaaaag cccgcactgt caggtgcggg cttttttctg tgtttcctgt
    15361 acgcgtcagc ccgcaccgtt acctgtggta atggtgatgg tggtggtaat ggtggtgcta
    15421 atgcgtttca tggatgttgt gtactctgta atttttatct gtctgtgcgc tatgcctata
    15481 ttggttaaag tatttagtga cctaagtcaa taaaatttta atttactcac ggcaggtaac
    15541 cagttcagaa gctgctatca gacactcttt ttttaatcca caccgagaca tattgcccgt
    15601 tgcagtcaga atgaaaagct gaaaaatact tactaaggcg ttttttattt ggtgatattt
    15661 ttttcaatat catgcagcaa acggtgcaac attgccgtgt ctcgttgctc taaaagcccc
    15721 aggcgttgtt gtaaccagtc gaccagtttt atgtcatccg ccactgccag agtcgtcagc
    15781 agtgccatgg ctcgttcgcg taaagcttgc agttgatgtt tgtctgccgt tgtgtcactt
    15841 ttcgccggtt gttgtattaa tgttgctaat tgatagcaat agaccatcac cgcctgcccc
    15901 agattgagcg aaggataatc cgccaccatc ggcacaccag taagaacgtc agccaacgct
    15961 aactcttcgt tagtcaaccc ggaatcttcg cgaccaaaca ccagcgcggc atggctcatc
    16021 catgaagatt tttcctctaa cagcagcacc agttctactg gcgtagcgta gtaatgatat
    16081 ttcgcccgac tgcgcgcagt ggtggcaaca gtgaaatcga catcgtgtaa cgattcagcc
    16141 agtgtcggga aaactttaat attatcaata atatcaccag atccatgtgc gacccagcgg
    16201 gtggctggct ccaggtgtgc ctgactatcg acaatccgca gctcgctaaa ccccatcgtt
    16261 ttcattgccc gcgccgctgc cccaatattt tctgctctgg cgggtgcgac cagaataatc
    16321 gttatacgca tattgccact cttcttgctc aaataaccgc gaaccgggtg accactgtca
    16381 acttattacg cggtgcgaat ttacaaattc ttaacgtaag tcgcagaaaa agccctttac
    16441 ttagcttaaa aaaagctaaa ctatttcctg actgtactaa cggttgaatt gttaaaaaat
    16501 g
//
LOCUS       DAZKGF010000114        16424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZKGF010000114 DAZKGF010000000
VERSION     DAZKGF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.114"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..187
                     /locus_tag="ACHOQV_003407"
     CDS             <1..187
                     /locus_tag="ACHOQV_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFP9631722.1"
                     /translation="LRNQKMMPLSSVGISSVQHEEYYIVFGALSLKSSLEDILLEITS
                     LVDNALDLAEITEEYSH"
     gene            206..904
                     /locus_tag="ACHOQV_003408"
     CDS             206..904
                     /locus_tag="ACHOQV_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFP9631723.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            954..1613
                     /locus_tag="ACHOQV_003409"
     CDS             954..1613
                     /locus_tag="ACHOQV_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFP9631724.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFLRALGVDIPLTSLHII
                     G"
     gene            1631..2029
                     /locus_tag="ACHOQV_003410"
     CDS             1631..2029
                     /locus_tag="ACHOQV_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFP9631725.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2039..2677
                     /locus_tag="ACHOQV_003411"
     CDS             2039..2677
                     /locus_tag="ACHOQV_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFP9631726.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2680..3843
                     /locus_tag="ACHOQV_003412"
     CDS             2680..3843
                     /locus_tag="ACHOQV_003412"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP9631727.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            3927..5552
                     /locus_tag="ACHOQV_003413"
     CDS             3927..5552
                     /locus_tag="ACHOQV_003413"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFP9631728.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERMEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5660..5935)
                     /gene="yjfN"
                     /locus_tag="ACHOQV_003414"
     CDS             complement(5660..5935)
                     /gene="yjfN"
                     /locus_tag="ACHOQV_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFP9631729.1"
                     /translation="MKQFLASSSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6084..6413)
                     /gene="bsmA"
                     /locus_tag="ACHOQV_003415"
     CDS             complement(6084..6413)
                     /gene="bsmA"
                     /locus_tag="ACHOQV_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFP9631730.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6595..7344
                     /gene="yjfP"
                     /locus_tag="ACHOQV_003416"
     CDS             6595..7344
                     /gene="yjfP"
                     /locus_tag="ACHOQV_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9631731.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHSTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLADRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7341..8096)
                     /gene="ulaR"
                     /locus_tag="ACHOQV_003417"
     CDS             complement(7341..8096)
                     /gene="ulaR"
                     /locus_tag="ACHOQV_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFP9631732.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8204..9268)
                     /gene="ulaG"
                     /locus_tag="ACHOQV_003418"
     CDS             complement(8204..9268)
                     /gene="ulaG"
                     /locus_tag="ACHOQV_003418"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP9631733.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9623..11020
                     /gene="ulaA"
                     /locus_tag="ACHOQV_003419"
     CDS             9623..11020
                     /gene="ulaA"
                     /locus_tag="ACHOQV_003419"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9631734.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKFAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11036..11341
                     /gene="ulaB"
                     /locus_tag="ACHOQV_003420"
     CDS             11036..11341
                     /gene="ulaB"
                     /locus_tag="ACHOQV_003420"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFP9631735.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            11351..11815
                     /gene="ulaC"
                     /locus_tag="ACHOQV_003421"
     CDS             11351..11815
                     /gene="ulaC"
                     /locus_tag="ACHOQV_003421"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFP9631736.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            11829..12479
                     /gene="ulaD"
                     /locus_tag="ACHOQV_003422"
     CDS             11829..12479
                     /gene="ulaD"
                     /locus_tag="ACHOQV_003422"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9631737.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12489..13343
                     /gene="ulaE"
                     /locus_tag="ACHOQV_003423"
     CDS             12489..13343
                     /gene="ulaE"
                     /locus_tag="ACHOQV_003423"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFP9631738.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEISVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13343..14029
                     /gene="ulaF"
                     /locus_tag="ACHOQV_003424"
     CDS             13343..14029
                     /gene="ulaF"
                     /locus_tag="ACHOQV_003424"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFP9631739.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14158..14433)
                     /gene="yjfY"
                     /locus_tag="ACHOQV_003425"
     CDS             complement(14158..14433)
                     /gene="yjfY"
                     /locus_tag="ACHOQV_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFP9631740.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            14761..15156
                     /gene="rpsF"
                     /locus_tag="ACHOQV_003426"
     CDS             14761..15156
                     /gene="rpsF"
                     /locus_tag="ACHOQV_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP9631741.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15163..15477
                     /gene="priB"
                     /locus_tag="ACHOQV_003427"
     CDS             15163..15477
                     /gene="priB"
                     /locus_tag="ACHOQV_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFP9631742.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15482..15709
                     /gene="rpsR"
                     /locus_tag="ACHOQV_003428"
     CDS             15482..15709
                     /gene="rpsR"
                     /locus_tag="ACHOQV_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP9631743.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            15751..16200
                     /gene="rplI"
                     /locus_tag="ACHOQV_003429"
     CDS             15751..16200
                     /gene="rplI"
                     /locus_tag="ACHOQV_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP9631744.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
BASE COUNT         3879 a         3969 c         4440 g         4136 t
ORIGIN      
        1 attaagaaat cagaagatga tgccgttgtc atcggtaggg atctccagcg tgcaacatga
       61 agagtattac attgttttcg gtgcgttatc gcttaaatct tctcttgaag atatcctgct
      121 tgagataacc tcgctggtag ataacgcatt ggatctggct gaaattacag aagaatattc
      181 acactaatta caaggacggg tatttatggg aattttaaaa agtttattta cgctggggaa
      241 atcgtttatc tcccaggcgg aagaatccat tgaagaaacc cagggcgtgc gcatgctgga
      301 acagcatatt cgtgacgcta aagctgaact cgataaagcc ggaaaatctc gcgttgatct
      361 gctggcgcgg gtgaaattaa gtcacgataa gctgaaagat ttacgtgagc gcaaagccag
      421 tctggaagct cgtgcgctgg aagcgttaag caagaacgtt aatccgtcgt tgattaacga
      481 agttgctgaa gaaatcgcgc gccttgagaa tctcattacc gctgaagagc aagtgttgtc
      541 gaatctggaa gtctctcgtg atggcgtgga aaaagcagtt acagcgacag cgcagcgtat
      601 cgctcagttt gagcagcaaa tggaagtcgt taaagctact gaagccatgc agcgtgcaca
      661 acaggctgta acgacctcta ctgttggcgc atcttccagc gtttcgacag cggcagaatc
      721 cttaaaacgc ctgcaaacgc gtcaggccga acgtcaggct cgcctggatg ctgccgcaca
      781 gttggaaaaa gtcgcagacg gtcgcgatct tgatgaaaag ctggcggaag ccggaattgg
      841 cggtagcaat aaaagtagcg cccaggatgt attagcaaga ctgcaacgcc aacagggcga
      901 gtaatttttt gccagcctca ctgcaaggct ggcaactatt ttaaggataa aatatgtctg
      961 gttttttcca gcgtctgttt ggcaaggata ataagcctgc tatcgctcgc ggtcctctgg
     1021 gacttcatct caatagtggc tttacgctcg acacgttagc gtttcgtttg ctggaagatg
     1081 aattgctgat tgcgctgccg ggtgaggaat ttacggtagc cgctgtcagc cgcatcgatc
     1141 tgggcggcgg tagccagatt ttccgctact acacttcagg cgatgagttt ctgcaaatca
     1201 ataccactgg cggcgaagat attgatgaca ttgatgatat caagctcttt gtctatgaag
     1261 agagctacgg tatcagtaaa gagagtcact ggcgcgaggc gatcaatgcc aaagcgatgg
     1321 gggcaatgac cttaaactgg caggaaaaac gctggcagcg attttttaat agcgaagaac
     1381 cgggaaatat cgaacccgtc tacatgctgg aaaaagtaga aaatcaaaac catgctaaat
     1441 gggaggtcca taattttacc atgggctatc aacggcaagt gaccgaagat acttacgaat
     1501 atctgctgtt aaatggtgag gaatctttta atgatctcgg cgagccggag tggttatttt
     1561 tgcgcgcgct gggggtcgat atcccgctga catcacttca tattattggt taattacaag
     1621 gacgtttacg atgcatatac tggattcact tcttgccttt agcgcttatt tttttattgg
     1681 tgtggctatg gtgattattt ttctgtttat ctactccaaa attacaccgc acaacgaatg
     1741 gcagttaatc aaaaataata acaccgcggc ggcactggcg ttcagcggaa cattgttggg
     1801 gtacgtgatc cccttatcca gtgcggcaat caatgcggtg agtattccag actatttcgc
     1861 atggggcggg attgctctgg tgattcaatt actcgttttt gctggcgtca ggctttatat
     1921 gcccgcatta agcgaaaaaa ttattaatca caataccgca gcaggaatgt ttatgggaac
     1981 cgccgcgctg gctggcggta tttttaacgc agcttgtatg acatggtaat ggacgatcat
     2041 ggccagaaaa cgcaaatcca gaaataacaa aaatatcgga catggggcaa tcagtcgcat
     2101 tggtagaccg cataatcctt ttgagccgcg ccgcaatcgc tacgcacaaa aatacttaac
     2161 gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtggcg atagtggcga
     2221 tgtcgataac gacggcgacg gaacgtttta cgcgactgtg caggattgta ttgatgacgg
     2281 taataatgca gacatttgcg cgcgtggctg gaacaacgcc aaagcggcgt tttatgccga
     2341 tgttccgaag aatatgactc agcagaattg tcagtctaag tacgaaaatt gctactacga
     2401 caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc gggttattcg
     2461 taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg cttcgcgccc
     2521 cgtctggcgc aacacttctg gtgattactc ctggcgctcc ggttctggca aaaaagagtc
     2581 ttactcttcg ggcggcttta ccaccaaaaa agcgtcaacc gtttctcgcg gcggctatgg
     2641 tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca caacgttcct
     2701 gtgcggcggg atctggacca gatcgccgct gacaacggtt tcgactttca tatcatcgac
     2761 aatgaaatct attgggacga gagtcgagct taccgtttta ctctgcgcca gattgaagag
     2821 cagatcgaaa aaccgactgc ggaactgcat cagatgtgcc ttgaggtggt ggatcgcgcg
     2881 gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg ggatgtgatc
     2941 gccgaaagct ggcgcgcacg cgatccttcg ttgtatggcc gtatggattt tgcctggtgt
     3001 ggtaatgcac cggttaagtt gctggagtac aacgccgata cgccaacttc attgtacgag
     3061 tcggcttatt tccagtggtt gtggctggaa gatgcccggc gcagcggcgt tattccgcgc
     3121 gatgccgatc agtacaatgc cattcaggag cgcctgattt cacgctttag cgagctttac
     3181 agtcgggaac cgttttattt ttgctgctgt caggacaccg acgaagacag gagtaccgtg
     3241 ctgtacttgc aggactgcgc cgagcaggca gggcaggagt cgcggtttat ctacattgaa
     3301 gatctcggtt tgggcgtcgg cggtgtgctg accgatcttg atgataatgt catccagcgt
     3361 gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc gctgctgcgc
     3421 aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa taaagggcta
     3481 atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc ctggttcgat
     3541 ggtgaaaaac cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat ttactcgcgc
     3601 gaaggcggaa acgtcaccat tttcgacggt cagaataacg ttgttgacca cgctgatggt
     3661 gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg atttggcgat
     3721 agctacacac tcatcggtag ctggattgtc gatgatgaag cgtgcggaat ggggatccgt
     3781 gaagataaca cgttgatcac caaagacacc tcacgtttcg ttccgcatta cattgctgga
     3841 taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtgacca tactaatggt
     3901 gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccgtt tttaatcaac
     3961 ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctctgc gaagcggtaa
     4021 cgcgtgaagg tgctggctgg gatagcgatt ttctcgccag tattggtcag cagttaggga
     4081 cggctgaatc ccttgaactg gggcggctgg cgaatgtgaa tccgcctgaa ttattgcgct
     4141 acgatgccca ggggcggcgt ctggacgatg tgcgttttca ccccgcctgg cacctgctga
     4201 tgcaggcgct atgtaccaat cgggtgcaca atcttgcctg ggaagaagac gctcgctccg
     4261 gcgcatttgt ggcgcgcgcg gcgcgtttta tgttgcacgc acaggttgag gcagggtcgt
     4321 tatgtccgat aacgatgacc tttgccgcca cgccattgct gttacagatg ttacccgcgc
     4381 cgtttcagga ctggaccacg ccgctgttga gcgatcgcta cgattctcac ttattgccag
     4441 gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaaacag ggcggttccg
     4501 atgtcatgag caacaccacc cgcgcagagc gcatggaaga tggctcttat cggctggtgg
     4561 ggcataaatg gtttttctcg gtgccgcaaa gcgatgcgca tctggtgctg gcgcagacta
     4621 cgggcggttt gtcctgcttt tttgtgccgc gctttttgcc tgacgggcaa cgcaacgcga
     4681 ttcgcctcga gcggctgaaa gataagctgg gtaatcgctc taacgccagc tgcgaagtgg
     4741 agtttcagga tgccattggc tggttgttgg ggcaggaagg ggaaggaatt cgtctgatcc
     4801 tgaaaatggg tgggatgacg cgttttgatt gcgccctggg tagccatgcc atgatgcgcc
     4861 gtgcattttc gctagcgatt tatcatgcac atcaacgcca tgtttttggt aatccattga
     4921 tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt gaagggcaaa
     4981 cggcgttgct gtttcgtctt gctcgagcgt gggaccggcg tgccgatgcc aaagaagccc
     5041 tgtgggcgcg tttatttacg cctgcggcga aatttgtgat ctgcaaacga ggtatgccgt
     5101 ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag agcgagctgc
     5161 cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggttccggt aatattatgt
     5221 gcctggatgt actgcgcgtg ctcaataagc aagcgggtgt atacgactta ttgtcggaag
     5281 catttgtgga agtgaaaggg caggatcgct attttgatcg cgcggttcgt cgtttacagc
     5341 agcagctgcg taagccagct gaagaactgg ggcgagagat tactcatcag ctattcctgc
     5401 tgggctgcgg tgcgcaaatg ttgaaatatg cctctccgcc aatggcgcag gcgtggtgtc
     5461 aggtgatgtt agatacgcgc ggcggcgtac ggttgtcaga gcagatccag aatgatttat
     5521 tgctgcgggc gacgggagga gtgtgtttgt aagcgtatac gacgctggtt tcgattaact
     5581 caatgaaata tgtgaaaatt gttagccgga caaggcgctc gcgccgcatc cggcattgtt
     5641 catttgctgt agatgacact cacgcataca ggatcgcctg tacgcgccag ttatcaggct
     5701 gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca tcagccttta
     5761 acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact gagatctgac
     5821 ctatttcatt caggcctgtc acgcagtcag cactggcgaa ttctgcagac tgtgccgtgg
     5881 cgctcgcagg ataagtcact aattgcagcg aggatgaggc aaggaattgt ttcatcgtca
     5941 gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat aaacgcccag
     6001 gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa gcaatgtaaa
     6061 gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga ataccactgt
     6121 cctgtgacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt tgcagcgaca
     6181 gctttcgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat cgtgcttacg
     6241 ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg atggtcggtt
     6301 actggcggtg ctggctgtgg cgtaccttgt agtgcgctac aggcacttaa catcaacacc
     6361 aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac catagtgtag
     6421 tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta cgcgattttc
     6481 gaatgaaaac gtgatgaaag cgtaacccac atctcaacat tatgaactaa taccgccagc
     6541 agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag acagatgatt
     6601 gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc tgtcgggcaa
     6661 aaagataccc cgttaccgtg cgcgattttt tatcacggct ttacttcgtc cagtctggtg
     6721 tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat gccggatgcg
     6781 cccgatcacg gtagtcgttt tagtggcgac gcagcgcggc ggttaaatca attctggcaa
     6841 atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc cgaagaaaac
     6901 tggctgcgtg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat gacggcactg
     6961 ggaattaccg ctcgccactc cacggtgaaa tgtaccgcca gcatgatggg atcgggctat
     7021 tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc agcacagcag
     7081 aatgaattca ataacattgt cgcgccgctg gcagagtggg aagcgacaaa ccacctggaa
     7141 caacttgctg acagacctct actgctgtgg catggccttg acgatgacgt cgtgcctgcc
     7201 gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa actgctaacc
     7261 tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga tgctgccgtg
     7321 acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc ttccagttgt
     7381 tgcaggattt ccgggttagc atttttgccg gtgatcagca tatcgatttg atcggcacgg
     7441 ctaaaaagca ttcccgcgcg ttcgccgatc ttactgctat caaccagtac caccagtttc
     7501 cctaccacgc ttagcatctt ctgctctgcc attgccgtca gcatatcggt tttatacaac
     7561 ccttctgctg tcagcccttt gccgctggta aacatccagt gcccggcata gagactgttt
     7621 tcgctgcctt gcgggcttaa agtgatggac tgacttttgt tgtactgtcc gcccataatg
     7681 atcacgctgt catgctcctg atcgatcagg taatttgcca gcggtagata attagtgatg
     7741 atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga gccgcagttg
     7801 atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat acgtactttt
     7861 tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgtgggcg ctgctgggta
     7921 atagcttctg cgccattgcg cacttttttc agtttgccgc tttcgtcaag tttattgata
     7981 tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc aacggtcaca
     8041 aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc agtcatgagc
     8101 tattccgata aaatttgatt tttttagatg atatttgaaa tagccaggaa atactacgcc
     8161 ggatagcgcg aactactcca cgctatccgg tacaagcgac agattacagg aacgacttga
     8221 acggcagatc cggttcaata gtgaagcaat catcgaaacc acgcggatag tggtactcga
     8281 agttgtcttt atccagcggc caggtaaatt tgccgccgac ctgccagata aacggcttga
     8341 agccatactt caggcgatct tttttcatct cccacagcac gcggatctct tgcggatcgg
     8401 cctggaagtt tgaccagata tcgtggtgga acgggatcac tactttagca ttcagcgctt
     8461 cgcccatacg caagatatca gcactggtca ttttgtcggt aataccgcgt gggttttcgc
     8521 cgtatgaacc taaagccacg tcgatctgat gctcgttgcc atgttttgca tagtagttag
     8581 agtagtggga atcgccgctg tgatacagag aaccgcccgg tgttttgaac agatagttta
     8641 ctgcgcgatc gtccatgccg tctggcagca cgccagccgc tttttgatcc gctggtaggg
     8701 tgatcagtgc agtacggtcg aacgcgtcaa gggcatgaat ttcaatgtct ttcactttta
     8761 cgacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag ccaatccata
     8821 aatccacgca ggttttcggt ccgataaacg gtacgtcatc agcacagttc tgcatcacgg
     8881 cagcagcgac gttgacgtcg atatgatcgt tgtgatcgtg agttgccagt accgcgtcga
     8941 tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgcaggttt ggctgcagtt
     9001 ttttcacgcc agccatgcgc tgcatctggt gaccctgttt catcagcggg ttaccgtgac
     9061 tttgtttgcc agtaccgcac cagaaatcaa cgcaaacgtt ggtgccacct tccgatttca
     9121 gccagatccc ggtgcagcct agccaccaca tcgcgaatgt gccaggggcg acctgctctt
     9181 gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg atccaggatt
     9241 ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt tcaattcgaa
     9301 tcaacatgtg attggttttg attaatcctg acactatttt ttcaggaagg caatgaccat
     9361 tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga tttaatggaa
     9421 tgtgtaattc aattaactaa atgaatttaa atgaataatt atttcgttgt gtgaatccca
     9481 ctctatccat gtggaattat ttgcgggtcg cgtcacattt aatcataaat aatcttgttg
     9541 tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgtgga atacccggtt
     9601 acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg tgttttttaa
     9661 ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg gctacatcct
     9721 actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa ttggtttcat
     9781 gttgttgcag gcaggatccg gcatcctcac cagcaccttc aaaccggtgg tggcgaaaat
     9841 gtccgaagtc tacggcatta acggcgcaat ttccgatact tacgcgtcga tgatggcaac
     9901 catcgaccgc atgggcgatg cctatagctg ggtaggttac gcggtgttgt tagcgctggc
     9961 actgaacatc tgttacgtgc tgctgcgtcg cattaccggt attcgtacca tcatgctgac
    10021 tggtcacatc atgttccagc aggcggggct gattgccgtc acgctgttca tctttggcta
    10081 ctccatgtgg acgaccatta tctgcaccgc gattctggtt tcgctctact ggggcattac
    10141 ctccaacatg atgtacaagc cgactcagga agtgacggac ggctgcggtt tctccatcgg
    10201 tcaccagcaa cagtttgcat catggattgc ctataaattt gcgccgttcc tcggcaaaaa
    10261 agaggaaagc gttgaagacc tcaaactgcc aggctggctg aatattttcc acgacaacat
    10321 cgtctccacg gcgattgtca tgaccatctt ctttggtgcc attctgctct ccttcggtat
    10381 cgacaccgtg caggcgatgg caggcaaagt gaactggacg gtgtacatcc tgcaaactgg
    10441 tttctccttc gcggtggcga tcttcatcat cactcagggt gtgcgcatgt ttgtggcgga
    10501 actctctgaa gcgtttaacg gtatctccca gcgtctgatc cctggtgcgg ttctggcgat
    10561 tgactgtgcg gctatttata gcttcgcgcc gaacgccgtg gtctggggct ttatgtgggg
    10621 caccatcggt cagctgattg cggttggcat cctggtcgcc tgcggttcct cgatcctgat
    10681 tatcccaggc tttatcccga tgttcttctc caacgccact attggcgtgt tcgctaacca
    10741 cttcggcggc tggcgtgcgg cgctgaagat ttgtctggtg atggggatga tcgaaatctt
    10801 tggctgcgtc tgggcggtga aacttaccgg tatgagtgcc tggatgggta tggcggactg
    10861 gtcgattctg gcaccgccga tgatgcaggg cttcttctcc atcggtatcg cctttatggc
    10921 tgtcatcatt gtaattgcac tggcttatat gttcttcgct ggccgcgcac tgcgcgcaga
    10981 agaagatgca gaaaaacaac tggcagaaca gtctgcttaa taaggagttt tgattatgac
    11041 cgtacgtatt ctggctgtgt gtggcaacgg acaaggcagt tccatgatca tgaaaatgaa
    11101 agtggaccag tttttaaccc agtcgaacat tgaccacacg gtaaacagct gcgcggttgg
    11161 cgaatacaaa agcgagttga gcggcgcgga tatcatcatc gcttctaccc atattgcggg
    11221 cgaaatcact gtgaccggca acaaatatgt ggttggcgtg cgcaatatgc tctctcctgc
    11281 cgactttggc ccgaaattgc tggaagtgat caaagagcat tttccgcagg acgtgaagta
    11341 aggacgcgcc atgaaattac gtgattcgct ggcggagaat aaatccatcc gcctgcaggc
    11401 tgaagctgag acatggcagg acgcagtaaa aatcggcgtt gacctgctgg tggcggcgga
    11461 tgttgtcgag ccgcgttact accaggcgat tctggatgcg gttgaacagc acggtccgta
    11521 ctttgtgctc gcacccggcc tggcaatgcc gcacggtcgc ccggaagagg gcgttaagaa
    11581 aaccggtttt gcgctggtga cgctgaaaaa gccgctggag ttcaaccacg aagataacga
    11641 tccggtggat atcctcatca ccatggcggc ggtcgatgcc aacactcacc aggaagtggg
    11701 catcatgcag atcgtcaatc tgtttgaaga tgaagagaat ttcgaccgtt tacgtgcctg
    11761 ccgtaccgag caggaagtac tggatctcat tgaccgcacc aacgcggcag cttaagaagg
    11821 aattgaaaat gtcattaccg atgttgcagg tcgcgctgga caaccagact atggatagcg
    11881 cctatgaaac cactcgcctg attgccgaag aagtcgacat tatcgaagtg ggtaccattc
    11941 tgtgcgtggg cgaaggcgtg cgtgcggttc gcgatctgaa agcgctttac ccgcacaaaa
    12001 tcgtactggc agacgccaaa attgccgatg cgggcaaaat cctttctcgt atgtgcttcg
    12061 aagctaacgc tgactgggtg accgtgattt gctgtgcgga tatcaacaca gctaaaggtg
    12121 cgctggacgt ggcaaaagag tttaacggcg acgtgcagat cgaactgacc ggttactgga
    12181 cctgggaaca ggcgcaacag tggcgcgacg caggcattgg gcaggtggtt tatcaccgca
    12241 gccgtgacgc acaggccgca ggtgtggcgt ggggcgaagc ggacattacc gcgatcaaac
    12301 gtctttccga tatgggcttc aaagtcaccg tcaccggtgg tctggcgctg gaagatctgc
    12361 cgttgtttaa aggcattccg attcatgtgt ttatcgctgg tcgcagcatt cgtgatgccg
    12421 cttctccggt ggaggccgca cgtcagttca aacgttccat cgctgaactg tggggctaag
    12481 gagcggatat gttgtccaaa caaatcccgc ttggcatcta tgaaaaagcg ctccccgccg
    12541 gggagtgctg gctggaacgg ctgcaactgg caaaaacgtt aggcttcgat tttgtcgaga
    12601 tttcggtaga tgaaacggac gatcgcctgt cgcgcctcga ctggagccgc gagcagcgtc
    12661 tggcgctggt gaacgcgatt gttgaaaccg gcgtgcgcgt gccgtccatg tgcctttctg
    12721 ctcatcgtcg tttcccgctg ggcagcgaag atgacgcagt gcgggcgcag gggctggaga
    12781 ttatgcgcaa agctatccag ttcgcccagg atgtcggtat tcgcgtgatc caactggcgg
    12841 gctacgacgt ttactatcag gaagccaata acgaaacgcg tcgtcgtttc cgtgacggcc
    12901 tgaaagagag cgttgagatg gcaagccgcg cgcaggtgac gctggcgatg gagatcatgg
    12961 attacccgtt gatgaactcc atcagcaagg cgctgggtta cgcgcactat cttaacaacc
    13021 cgtggttcca gctctacccg gatatcggca acttgtcggc gtgggacaac gacgtgcaga
    13081 tggagttgca ggccgggatc gggcatatcg tcgcggtgca tgtgaaagac accaaaccag
    13141 gcgtcttcaa aaacgtcccg tttggcgaag gtgtagtgga tttcgaacgt tgcttcgaaa
    13201 cgctcaaaca gagtggttat tgcgggccgt acctgattga gatgtggagc gaaacggcgg
    13261 aagacccggc ggcagaagtg gcgaaagcgc gtgattgggt gaaagcgcgc atggccaaag
    13321 ccggcatggt ggaggcggca taatgcaaaa gctaaaacag caggtatttg aagccaatat
    13381 ggatctgccg cgctacgggc tggtgacctt tacctggggc aacgtcagcg ccattgaccg
    13441 cgaacgcggg ctggtggtga tcaagcccag cggcgttgcc tacgaaacca tgaaagcaga
    13501 cgatatggtg gtggttgata tgagcggcaa ggtggtggaa ggggagtatc gtccgtcttc
    13561 cgacaccgcg acgcatcttg aactctaccg ccgttatccg tcgcttggcg gcattgtcca
    13621 tacccattcc acgcatgcca ccgcatgggc gcaggcgggg ctggctatcc cggcattagg
    13681 caccacgcac gctgactact tctttggcga tatcccctgt acgcgcgggt taagcgaaga
    13741 agaagtgcag ggggaatacg agctaaacac tggcaaagtg attatcgaaa cgctgggtga
    13801 cgccgagccg ctgcatacgc cgggaattgt ggtgtatcag cacgggccat tcgcctgggg
    13861 gaaagatgct cacgatgcgg tgcataacgc ggtggtgatg gaagaagtgg cgaaaatggc
    13921 gtggattgcc cgcggcatta acccgcaact gaatcacatc gacagctatc tcatgaataa
    13981 acacttcatg cgtaagcacg gtcccaacgc ctattacggg cagaagtaaa acacgcgctg
    14041 cggagatttc cttcttcggg agataactgg tctaagtccg cagccgtttt tcaaaaaaag
    14101 ccccctgaaa agggggcaaa gcaaactatg gcaatgtttc gttggttata cctggtgcta
    14161 gcgataaata tccgcgctgg cgtgcatatt gccgttactc cccggttccc gcatcagaat
    14221 cacgtggtag tacgttgcgc cctgcgcatc tgtctcttca tttaatgcct gacgcgcttc
    14281 gctttcagtg gcgaaattat gattgatata aatcacgcct aagctttgca catcgtccat
    14341 atttctggcc tggtggttat taatttcaat ggctgcccat gtatttgcac ttagcaaaag
    14401 cacagccaga agggctaaaa cacggctgaa catagatacc tcctcgacgg ctgactttgt
    14461 gtgctctcct cttgtggtga tcttctgatt taattttaat caatgataaa gaagtggatg
    14521 gtgaccattt ctgatgcagt tgttcaaaaa aacaccatga tgaagtgtga tgaacttcaa
    14581 atcagcgtgt tagtacgatt tggtgaagga tgagatttat ttgtcggctg gccttgagtt
    14641 tagcggggta tgtcagtact ataacgcgtc atttttcagc cgacctttaa cacgttcctt
    14701 gcctccccgg gattcggctg acccagacag gaggctgaat aatccgtaag gagcaattcg
    14761 atgcgtcatt acgaaatcgt ttttatggtc catcctgacc agagcgaaca ggttccgggc
    14821 atgatcgagc gctacactgc tgccatcact ggtgcagaag gcaagatcca ccgtctggaa
    14881 gactggggcc gccgtcagct ggcttacccg atcaacaaac tgcacaaagc acactacgtt
    14941 ctgatgaacg ttgaagctcc gcaggaagtg atcgatgagc tggaaactac cttccgcttc
    15001 aacgatgccg ttatccgcag catggttatg cgtaccaagc acgctgttac cgaagcatct
    15061 ccgatggtta aagcgaaaga cgagcgccgt gagcgtcgcg atgatttcgc aaacgaaacc
    15121 gctgatgatg ctgaagctgg ggattctgaa gagtaatttc tgatgaccaa ccgtctggtg
    15181 ttgtccggca ccgtgtgcag gactcccctt cgaaaggtca gtccatcagg aattcctcac
    15241 tgccagttcg tgcttgagca tcgttctgtg caggaggaag ccggctttca ccggcaggca
    15301 tggtgccaaa tgcccgttat tgttagcgga cacgaaaacc aggccattac tcacagtata
    15361 acggtcggca gtcgcataac cgttcagggg ttcatttcat gccataaggc aaagaacgga
    15421 ctgagcaaaa tggttttgca tgccgagcag attgaattga tagattctgg agactagcca
    15481 tatggcacgt tatttccgtc gtcgcaagtt ctgccgtttc accgcggaag gcgttcaaga
    15541 gatcgactat aaagatatcg ctacgctgaa aaactacatc accgaaagcg gtaagattgt
    15601 cccaagccgt atcaccggta cccgtgcaaa ataccagcgt cagctggctc gcgctatcaa
    15661 acgcgctcgc tacctgtccc tgctgccgta cactgatcgc catcagtaat cggtcacggt
    15721 ccattaatac gactttgaga ggataaggta atgcaagtta ttctgcttga taaagtagca
    15781 aacctgggta gcctgggtga tcaggtaaac gttaaagcgg gctatgctcg taacttcctg
    15841 gtaccgcagg gtaaagctgt tccagctacc aagaaaaaca ttgaattctt cgaagcacgt
    15901 cgcgctgaac tggaagctaa actggctgaa gttctggcag cagctaatgc tcgcgctgag
    15961 aaaatcaatg cactggaaac tgttaccatc gcgtctaaag ctggcgacga aggtaaactg
    16021 ttcggttcca tcggtactcg cgacatcgct gacgctgtaa ctgcagctgg cgttgaagtg
    16081 gctaagagcg aagttcgtct gccgaacggc gttctgcgta ccactggcga acacgaagtg
    16141 agcttccagg ttcacagcga agtattcgcg aaagtgatcg taaacgtagt agctgaataa
    16201 ttcgttattc aacgagacgt aaaaacgccg accagtggtc ggcgttttgc ttttaagtga
    16261 taaaaaccgc agaaacagac gttccctcca taaatctctt gctattcctt ttataaaaag
    16321 ttcgcagtac ccatacctgg cccccagcct cgccgctggt agttcgtgtc atgtttttta
    16381 cgcgtaaaac acgacaaaag catcaataca gcgatcgtct cact
//
LOCUS       DAZKGF010000115        16389 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZKGF010000115 DAZKGF010000000
VERSION     DAZKGF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.115"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            123..506
                     /gene="grcA"
                     /locus_tag="ACHOQV_003430"
     CDS             123..506
                     /gene="grcA"
                     /locus_tag="ACHOQV_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFP9631745.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(562..1149)
                     /gene="eamB"
                     /locus_tag="ACHOQV_003431"
     CDS             complement(562..1149)
                     /gene="eamB"
                     /locus_tag="ACHOQV_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFP9631746.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGITALSTFVLPQTQALSWIIGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            1252..2133
                     /gene="yfiE"
                     /locus_tag="ACHOQV_003432"
     CDS             1252..2133
                     /gene="yfiE"
                     /locus_tag="ACHOQV_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9631747.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFTVANELASGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            complement(2166..3500)
                     /gene="srmB"
                     /locus_tag="ACHOQV_003433"
     CDS             complement(2166..3500)
                     /gene="srmB"
                     /locus_tag="ACHOQV_003433"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFP9631748.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            3632..4369
                     /gene="trmN"
                     /locus_tag="ACHOQV_003434"
     CDS             3632..4369
                     /gene="trmN"
                     /locus_tag="ACHOQV_003434"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFP9631749.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(4354..5976)
                     /gene="nadB"
                     /locus_tag="ACHOQV_003435"
     CDS             complement(4354..5976)
                     /gene="nadB"
                     /locus_tag="ACHOQV_003435"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFP9631750.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDDRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRCMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            6232..6387
                     /gene="rseD"
                     /locus_tag="ACHOQV_003436"
     CDS             6232..6387
                     /gene="rseD"
                     /locus_tag="ACHOQV_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFP9631751.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            6384..6959
                     /gene="rpoE"
                     /locus_tag="ACHOQV_003437"
     CDS             6384..6959
                     /gene="rpoE"
                     /locus_tag="ACHOQV_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFP9631752.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            6992..7642
                     /gene="rseA"
                     /locus_tag="ACHOQV_003438"
     CDS             6992..7642
                     /gene="rseA"
                     /locus_tag="ACHOQV_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFP9631753.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            7642..8598
                     /gene="rseB"
                     /locus_tag="ACHOQV_003439"
     CDS             7642..8598
                     /gene="rseB"
                     /locus_tag="ACHOQV_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFP9631754.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            8595..9074
                     /gene="rseC"
                     /locus_tag="ACHOQV_003440"
     CDS             8595..9074
                     /gene="rseC"
                     /locus_tag="ACHOQV_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFP9631755.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DIAALCGAILGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            9272..11071
                     /gene="lepA"
                     /locus_tag="ACHOQV_003441"
     CDS             9272..11071
                     /gene="lepA"
                     /locus_tag="ACHOQV_003441"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP9631756.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            11087..12061
                     /gene="lepB"
                     /locus_tag="ACHOQV_003442"
     CDS             11087..12061
                     /gene="lepB"
                     /locus_tag="ACHOQV_003442"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9631757.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            12333..13013
                     /gene="rnc"
                     /locus_tag="ACHOQV_003443"
     CDS             12333..13013
                     /gene="rnc"
                     /locus_tag="ACHOQV_003443"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP9631758.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            13010..13915
                     /gene="era"
                     /locus_tag="ACHOQV_003444"
     CDS             13010..13915
                     /gene="era"
                     /locus_tag="ACHOQV_003444"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP9631759.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            13927..14655
                     /gene="recO"
                     /locus_tag="ACHOQV_003445"
     CDS             13927..14655
                     /gene="recO"
                     /locus_tag="ACHOQV_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP9631760.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGESVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            14667..15398
                     /gene="pdxJ"
                     /locus_tag="ACHOQV_003446"
     CDS             14667..15398
                     /gene="pdxJ"
                     /locus_tag="ACHOQV_003446"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFP9631761.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIPVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            15398..15778
                     /gene="acpS"
                     /locus_tag="ACHOQV_003447"
     CDS             15398..15778
                     /gene="acpS"
                     /locus_tag="ACHOQV_003447"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP9631762.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            16200..16280
                     /gene="shoB"
                     /locus_tag="ACHOQV_003448"
     CDS             16200..16280
                     /gene="shoB"
                     /locus_tag="ACHOQV_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFP9631763.1"
                     /translation="MTDCRYLIKRIIKIIIAVLQLILLFL"
BASE COUNT         4047 a         4024 c         4351 g         3967 t
ORIGIN      
        1 attgatttaa atcaaagatt caagggtgtt tgaggagtat atatacactc aagcaacaat
       61 ggttttacca attggccgcg acaggctgaa caaatcaaat aattttgccg gggaggcatc
      121 acatgattac aggtatccag attactaaag ccgctaacga cgatctgctg aactctttct
      181 ggctgctgga cagcgaaaaa ggcgaagcgc gttgcatcgt tgcgaaagca ggttttgcag
      241 aagacgaagt ggttgcagta agcaaactgg gtgacattga gtaccgtgaa gttccagtag
      301 aagtgaaacc agaagttcgc gttgaaggtg gtcaacatct gaacgtaaac gttctgcgtc
      361 gtgaaactct ggaagatgca gttaagcatc cggaaaaata tccgcagctg accatccgtg
      421 tatccggtta tgcagttcgc tttaactctc tgactccgga acagcagcgc gacgttattg
      481 ctcgtacctt tactgaaagc ctgtaaggct taagtagcaa gttactaagc ggaagagggc
      541 gacttcttcc gctttttttc gttaatagaa aatgcgtacc gcgcaataga ccagcagcag
      601 cgcgagcacg atatttaact ggcgaccata ctggcgaaac aatcgctgaa acaggtgccc
      661 cgccagcgcc cagcacacat tgccaaacgt cccaatcatc gccagcaaaa cgctgacgcc
      721 aattatccag cttagcgcct gcgtttgcgg caggacaaac gtcgacagtg ctgtaatgcc
      781 atacaaaatg attttgacgt tcacaaactg caaagcaaag ctggcccaaa aactgattgg
      841 ttttgtctga agcccatctt cctttgttgg actggtagcg attttccacg ccagccagac
      901 aatatatgcc gcccccgccc aactcaacag atgcactgct gccgggtcga taacagccag
      961 tgaaaacgaa attcccgcgc atagcaacat gacgattaaa aatcccagac tcatccctgc
     1021 cagcacgcga gtactctgac gaaatccgtg cgttgtggct gagctaaggg cgagaatatt
     1081 gttcggtcct ggcgtcatgg cggtaatcag ggtgtaagtc caaaaagcac ttaaaagggt
     1141 cggtgtcact tgttatctcc tctgttttgg tagacagtac ccgacacgga acatttaaaa
     1201 aaatgaatgt ttttacattc taaattcgat attttcgata ggtttggggt tatggatctg
     1261 cgccgtttta ttacacttaa aaccgtggtg gaagagggtt cctttttgcg agcttcgcaa
     1321 aaattgtgct gcacacaatc gacggtgact tttcatattc agcagcttga gcaggagttc
     1381 tcagtccagt tatttgagaa aattggtcga cgaatgtgcc ttacccgcga aggaaaaaag
     1441 ctgctgccgc acatttatga actcaccaga gtgatggata cccttcgtga ggctgccaaa
     1501 aaagagtcgg atccggacgg ggaactacgc gttatttcag gcgaaacgtt gctctcttat
     1561 cgaatgccgc aggtgttgca gcgttttcgg caacgcgctc caaaagtgcg tttatcgttg
     1621 cagtcgctga actgttacgt gatccgtgat gcgctactga atgatgaggc cgatgtcggg
     1681 gttttttatc gcgtagggaa tgatgatgct ttgaaccgac gagagttggg tgaacaaccg
     1741 ctggtgctgg tggcttcacc gcaaattgca gatgtcgatt ttactgagcc gggaagacat
     1801 aacgcctgta gctttattat caacgaacca caatgtgtct tccggcagat atttgagagc
     1861 acgctgcgcc agcgacggat cacggtggaa aacaccattg agcttataag tatcgaaagc
     1921 atcaagcgtt gtgtcgcggc aaatatcggg gtcagttacc tgccgcgctt tacagtggca
     1981 aatgaacttg cgagtggcga attaatcgaa ttgccttttg gcgaacagtc gcagaccatt
     2041 acggccatgt gcgcacacca tgctggaaaa gcggttagcc cggcgatgca cacttttatt
     2101 cagtgtgttg aagagtgttt tttgccggga taaaaacaat gccgggctta aacccggcat
     2161 tgagattact cttctgtcgt ttgcggtggc acgcccgttc cgcttggttt acggcgctta
     2221 ccaatatttt tagtgtcgcg atggcgtttt ttcacccgcg gcttctcttt ctctttagct
     2281 tttttcttct cggcacgttt agccaacact ttcttcgatg gcttgccggt ctgcttctcg
     2341 cttggcgcac gcgttttcgg acgtaactca tcaattacgc gagctttaat tggctcttca
     2401 atataacggc caactttgcc cagcagcaga tggtcatggg cttccaccag cgaaatcgcg
     2461 gtgcctttac gaccggcgcg cgcggtacgt ccgatacggt gcaaataagt atcgccactg
     2521 cgcggcatat cgaagttaaa gacgtggctg acgtcaggaa tgtcgatacc gcgcgcggca
     2581 acatcggtag caaccagtac gtttacgcgg ccttcggtca agcgtttaat cgcttcgtta
     2641 cgcttgcctt gtaccatctc accttcgaga tagcagttgt tgatgcctgc ttcgcgcagc
     2701 cagtttgcca gctcatgcac acgctcacgc ttacgcacaa acacaattga gcgggtcgct
     2761 tccggctgtt ttaacagatg caccagcaac gcggttttat gctcaagatc atcggcgcgg
     2821 taataccact gatgaatttt tttgcgctca cgggtggacg gattggcaga aacctccacc
     2881 ggatcttcca gcagacgctc ggcaaagtcc tgaatcgcat cgccttccag agttgccgaa
     2941 aagagcaggg tctgtttacg ccagcgcgtt tcgccagcaa tatgttcgat atcctgggcg
     3001 aagcccatat ccagcatacg gtctgcttcg tcgaggatca gcgtttcaac cgcgcggcaa
     3061 tcgaagttct cttcttttat gtattgcagc agacgtccgg tcgtggcgac cacgatgtcc
     3121 tgattttcgc tgaacacttc cgcgtggttc atataggcta cgccgccggt gatggtggca
     3181 atatccagat gcgtatgttt cgccagttcg cgggcatgat cggccacctg catcgccagc
     3241 tcgcgagttg gggtgaggat caaaatacgc ggcggaccgg atttcttacg tgggaaatcg
     3301 agcaggtgct gcaacgctgg cagcagatac gccgccgttt taccggtgcc tgtcggcgca
     3361 gaaccgagta catcacggcc atcgagcgca ggcggaatgg cggcagcctg aatggcggtc
     3421 gggcgagtga aacctttatc ctggagggct tccagcaggc tttcgtcgag ttcaagttcg
     3481 gaaaaagtcg ttacagtcat gttctacctc tgtgtggggc gctgattata gacgttacgg
     3541 ctgcaatctt catctgtttg tatggatatc gcttctcggg tattgttctc acccggtgct
     3601 atcgccgttt ttcctgcaag gttttatttt catgtcacag tctacatccg tgcttcgtcg
     3661 taatggattt acttttaaac agttttttgt tgctcacgat cgttgtgcga tgaaagtggg
     3721 aacggatggc attttgctgg gcgcatgggc accggtggct ggggtaaaac gttgccttga
     3781 tatcggcgcg ggtagcgggt tgctggcatt aatgctggcg cagcgaaccg atgacagcgt
     3841 aatgattgat gcagttgaac tggaaagtga agctgccgcg caggcgcagg aaaatatcaa
     3901 ccagtccccg tgggcagagc ggattaacgt ccatacggcg gatattcagc agtggatcac
     3961 acagcagaca gtacgcttcg atttaatcat cagtaaccca ccttactatc agcagggagt
     4021 agagtgcgcg acacctcaac gggaacaggc tcgctatacc actacgcttg atcacccatc
     4081 gttgctgacc tgtgcggcgg agtgcattac cgaagaggga tttttctgcg tggtgctgcc
     4141 agagcagatt ggtaatggtt ttactgaact ggcattaagt atgggctggc atttacgttt
     4201 gcggacggat gtggcggaaa acgaagcgcg actgccgcat cgggtgctgc tggcattctc
     4261 cccgcaggcg ggagaatgct ttagcgatcg cttagtcatt cgtgggccag accagaacta
     4321 ttccgaagcg tatacggcgc tgacccaggc tttttatctg tttatgtaat gattgccggg
     4381 ggaaaggatc gacggaccgg aatgggtgag cagttccgga taatccagcg tgaaatgcaa
     4441 cccccgactc tctttacgca tcattgcaca gcgaacaatc aactcggcaa cctgtaccag
     4501 attacgcagc tccagcaaat tatttgagac gcggaaatgg gcgtaatatt cgtctatttc
     4561 ttgctggagc atggttatcc gccgcagggc gcgttccagg cgcttcgttg tgcgcacaat
     4621 gccgacgtaa tcccacataa acagacgtag ctcgtgccag ttatgttgaa tcaccacccg
     4681 ttcatcaggg ttctcaacgc ggctttcatc ccacggcggt aacgtactga cgccgtgggc
     4741 ataaggcata catctggtga tatcttctgc cgccgaccag ccatagacca gacactccag
     4801 caaggagtta gaggccatgc ggttagcgcc gtgtaagccg gtataactca cttcgccaat
     4861 ggcatacaaa ccctcgacgt ccgtacgccc atgatcatcc accatcacac caccgcaggt
     4921 atagtgtgca gcaggcacaa tcggtactgg ttcttgtgtg agatcaatcc ccagcccgag
     4981 caatttttca taaatcatcg ggaaatgctg gcgaataaaa tcggcgggct tatggctgat
     5041 gtcgaggaac atacaatccg cgccgaggcg tttcatttca tggtcaatag cgcgggcgac
     5101 aatatcgcgc ggggccagtt cgccgcgatc atcaaaatcg ggcataaaac gcgtaccatc
     5161 cgggcgcttg agataagcgc cttcgccgcg cagtgcttct gttaacagga aattgcgtgc
     5221 ctgtgggtga tacagtgcgg tagggtggaa ctgattaaat tcgagattgg caacccggca
     5281 gcctgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
     5341 ctgataaacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
     5401 caccgtttct ttatttcggt tccataccca cgcgccaaca acccgtcgcg tgcccggcag
     5461 gccaattttg tcagaaacaa tcagatcaac cgcgttgctg cgctccagca cgcgaatatt
     5521 cggatggttc agcgccttgc tcaccagcgt ggtttctact tctctaccgg tggcgtcggc
     5581 ggcatgaaga attcgacggt gactatgtcc accttcacgg gtcagatggt agctttctgc
     5641 accattcggt tgaatgtggg tatcaaacaa caccccctgg tcgattagcc attgcacaca
     5701 ggatcgtgca ttgctggcga caaattcaac tgcatgacga tcgcaaatac cagccccggc
     5761 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtc tcatcaaaca cggcggctat
     5821 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
     5881 ctgatgctgg tcagccaggc gtagcgccag tgaaagtcca gctgcgccgc taccgataat
     5941 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
     6001 tggtttggtc agcatagcat catgttgtgc gattaaacac ctgctatttt aatatttgtt
     6061 acagtgacta aacacgctga ctcagggcgg cgagaaaaag agaagttact ggctggtgga
     6121 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
     6181 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
     6241 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     6301 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     6361 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     6421 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     6481 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     6541 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     6601 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     6661 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     6721 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     6781 gaactattga gtccctcccg gaagatttac gcatggcaat aaccttgcgg gagctggatg
     6841 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     6901 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     6961 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     7021 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     7081 gaaaacctgg gaaagctatc acttaatccg tgactcaatg cggggtgata ctcccgaggt
     7141 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     7201 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaga aaatgccatt
     7261 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag ccgcatgcgt
     7321 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     7381 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     7441 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     7501 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     7561 gcttcagttt gagcaggcgc aaactcagca agccgctgta caggtgccag gaattcaaac
     7621 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     7681 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     7741 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     7801 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     7861 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     7921 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctaccatcg
     7981 ctcatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     8041 acgcgtattg ctgatcgtct ttgcgaagtc attcgtgtgg ttgcccgaga tggtacacgc
     8101 tacagctaca tcgtgtggat ggacaccgaa tcgaaattac cgatgcgggt tgatctcctt
     8161 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattg cttttaatgt caatcaggat
     8221 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     8281 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     8341 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     8401 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     8461 gatcagatgt tgcgcaccgg gcgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     8521 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     8581 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctgggaa aacgggcagg
     8641 cgctggttag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgt gccggttgcg
     8701 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     8761 atgagccgtt agtgccgggg caaaaagtag aattaggaat cgccgaaggc agcctgctta
     8821 gctccgcatt actggtttat atgtcgccgt tggtgggact attcctcatc gcttcactat
     8881 ttcagctact ctttgcttca gatattgcag cattatgcgg tgcgattctc ggtggcgttg
     8941 gtggattcct gattgcacgt ggctgctctc gaaagtttgc tgcccggtct gaatggcaac
     9001 cgatcatctt aagcgtggcc ttgccgcctg gccttgtgcg atttgaaaca tcttcggaag
     9061 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     9121 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     9181 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     9241 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     9301 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     9361 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     9421 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     9481 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttctcg
     9541 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtagaagc
     9601 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccggtact
     9661 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     9721 cgtcggcatc gacgccaccg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtaca
     9781 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggccc
     9841 gttgcaggca ctcattatcg actcctggtt cgacaactac ctgggcgttg tttcacttat
     9901 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     9961 gacctataac gccgaccgtc tgggcatctt cacgccgaag caggttgacc gtactgaact
    10021 gaaatgcggc gaagtaggct ggctggtatg tgcgattaaa gatatccacg gcgctccagt
    10081 aggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
    10141 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
    10201 ccgtgacgcg ctgggcaaac tcagcctgaa cgatgcttca ctgttctatg agccggaaag
    10261 ctccagcgcg ctgggctttg gtttccgctg cggcttcctc ggcctgctgc acatggagat
    10321 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
    10381 agtgtatgaa gttgaaacca cgtcaagaga agttatctac gtcgacagcc catccaagct
    10441 gccagcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
    10501 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
    10561 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
    10621 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
    10681 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
    10741 acgtgttgat gcgctggcgt tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
    10801 gctggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
    10861 agcgattggt acgcacatca ttgcgcgctc caccgtgaaa cagctgcgta aaaacgtact
    10921 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
    10981 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
    11041 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
    11101 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
    11161 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
    11221 actggataaa gcaacgctga agaaagttgc gccgaagcct ggctggctgg aaaccggtgc
    11281 ttctgttttt ccggtgctgg ctatcgtatt gattgtgcgt tcgtttattt atgaaccgtt
    11341 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
    11401 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
    11461 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
    11521 caagcgcgcg gtgggtttgc cgggcgataa agtcacttat gatccggtct caaaagagct
    11581 gacgattcaa ccgggatgca gttccggcca ggcgtgcgaa aacgcgctac cggtcactta
    11641 ctccaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
    11701 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
    11761 cgagcgtaaa gagacattgg gtgatgtgac gcaccgcatt ctgacagtgc cgattgcgca
    11821 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
    11881 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
    11941 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagcttcga
    12001 taagcaagaa ggcgaatggc cgactggtgt gcgcttaagt cgcattggcg gtatccatta
    12061 atagccatct tcgttcacgt tgtcgccgtt atggcgacaa cgtgaattat ttatgagata
    12121 aatctcccgt ggctaacgac atcccccgtc gttgtgtata gaatattccc ccgaagttta
    12181 aggttggcac ctccaggttg ccacggcaca cgaaacagcg ttggtcccca tataccggta
    12241 aactgaaact gcagtgaagc agttagccga gccatgtata tcaggtctgt ttcgtgtgct
    12301 gaattgttga cgcatttatt tattggtatc gcatgaaccc catcgtaatt aatcggcttc
    12361 aacggaagct gggctacact tttaatcatc aggaactgtt gcagcaggca ttaactcatc
    12421 gtagtgccag cagtaaacat aacgagcgtt tagaattttt aggtgactct attctgagct
    12481 acgttatcgc caatgcgctt tatcaccgtt tccctcgtgt ggatgaaggc gatatgagcc
    12541 ggatgcgcgc cacgctggtc cgtggtaata cgctggctga actggcgcgc gaatttgagt
    12601 taggtgagtg cttacgttta gggccaggtg aacttaaaag cggtggattt cgtcgtgagt
    12661 caattctcgc tgacaccgtc gaggcattaa ttggtggcgt attcctcgac agtgatattc
    12721 aaaccgtcga gaaattaatc ctcaactggt atcaaactcg tctggacgaa attagcccag
    12781 gcgataaaca aaaagatccg aaaacgcgtc tgcaggaata tttgcagggt cgccatctgc
    12841 cgctgccaac ttatctggta gtccaggtac gtggcgaagc gcacgatcag gaatttacta
    12901 tccactgcca ggtcagcggc ctgagtgaac cggtggttgg cacaggttca agccgtcgta
    12961 aggctgagca ggctgccgcc gaacaggcgt tgaaaaaact ggagctggaa tgagcatcga
    13021 taaaagttac tgcggattta ttgctatcgt cggacgtccg aacgttggca aatccacatt
    13081 gttgaacaaa ctgctggggc agaagatctc catcacctcc cgcaaggcgc aaacaactcg
    13141 tcaccgcatt gtggggatcc atactgaagg cgcgtatcag gcgatctacg ttgatacccc
    13201 aggcctgcat atggaagaaa aacgcgccat taaccgcctg atgaacaaag cggcgagcag
    13261 ctctatcggc gacgttgagc tggtgatttt tgtcgtagaa ggcacccgct ggacgccgga
    13321 cgatgaaatg gtgctcaaca aactgcgcga tggcaaagcg ccggtaatcc tcgcggtgaa
    13381 caaagtggat aacgtgcagg aaaaagccga tctgctgccg cacttacagt tcctggcaag
    13441 ccagatgaac ttcctggata ttgtgccgat ctctgccgaa accgggctga atgtcgacac
    13501 tatcgcagca atcgtgcgta agcatctgcc tgaagcgacc catcacttcc cggaagatta
    13561 catcaccgat cgctcacagc gttttatggc gtctgaaatc atccgcgaaa aactgatgcg
    13621 tttcctcggc gctgaactgc cgtactccgt gaccgtggag atcgaacgtt tcgtctctaa
    13681 cgaacgcggc ggttatgaca tcaacggttt gattctcgtt gagcgtgaag ggcagaagaa
    13741 gatggttatt ggcaacaaag gggccaagat caaaaccatc gggattgaag cgcgtaaaga
    13801 catgcaggaa atgtttgaag cgcctgttca ccttgagctg tgggtaaaag tgaaatccgg
    13861 ttgggccgac gacgaacgcg cactgcgcag tctcggttat gttgacgatc tttaagagta
    13921 actccgatgg aaggctggca gcgcgcattt gtcctgcata gtcgcccgtg gagcgaaacc
    13981 agcctgatgc tggacgtctt cacggaggaa tcgggtcgcg tgcgtctggt tgccaaaggc
    14041 gcacgctcta aacgctctac tctgaaaggt gcattacaac ctttcacccc tctcttgctt
    14101 cgcttcggtg ggcgtggtga agtcaaaaca ctgcgcagtg ctgaagctgt ctcgctggcg
    14161 ctgccattaa gcggtatcac gctttacagc ggtctgtaca tcaacgaact tctctcccgc
    14221 gtactggaat acgagacgcg cttctctgaa ctctttttcg attacttgca ctgcattcag
    14281 tcacttgcag gtgccactgg tacgccagaa cccgcgctgc gtcgctttga actggcactg
    14341 ctcgggcatc tgggctatgg cgtcaatttt acccattgtg cgggtagcgg cgagtcggta
    14401 gatgacacca tgacgtatcg ttatcgcgaa gaaaaagggt ttatcgcaag cgtcgttatc
    14461 gacaataaaa cgttcaccgg aaggcagtta aaagcgttaa acgcgcggga atttcctgac
    14521 gcagacacac tgcgcgccgc gaaacgcttt acccgcatgg cgcttaagcc gtatcttggc
    14581 ggtaaaccat taaagagcag ggaactgttc cggcagttta tgcctaagcg aacggtgaaa
    14641 acacattatg aatgatgagg attgtcatgg ctgaattact gttaggcgtc aacattgacc
    14701 atattgcaac gctgcgtaac gcacgcggta ctgcttaccc ggatccggtg caggcggcgt
    14761 ttattgctga gcaggcggga gcggacggca ttaccgtgca tttacgtgaa gatcgccgcc
    14821 acattactga ccgcgatgtg cgcatcctgc gtcagacgct ggatacccgc atgaatctgg
    14881 agatggcggt gaccgaagag atgctggcga tcgccgttga gacgaagcca catttttgct
    14941 gtctggtacc ggaaaagcgt caggaagtaa caaccgaagg cggcctggac gtcgcagggc
    15001 agcgtgacaa aatgcgcgat gcctgtaaac gtctggcgga ggccgggatc ccggtttctc
    15061 tgtttatcga cgccgatgaa gagcagatca aagctgcggc agaggttggc gcaccgttta
    15121 tcgagatcca caccggttgc tatgctgatg ccaaaactga cgccgaacag gcgcaagagc
    15181 tggcgcgtat cgccaaagcc gcgacctttg ccgctagtct cggtctgaaa gttaacgctg
    15241 gtcacggtct gacctatcac aacgtgaaag ctattgccgc catccctgag atgcatgaac
    15301 tgaatatcgg tcatgccatt attggtcgtg cagtgatgac cggactgaaa gatgcggtgg
    15361 cagaaatgaa gcgtctgatg ctggaagcgc gtggctaatg gcaatattag gtttaggtac
    15421 ggatattgtg gagatcgctc gcatagaagc ggtgatcgcc cgatccggtg agcgcctggc
    15481 acgccgcgta ttaagcgata acgaatgggc aatctggaaa acgcaccacc agccggtgcg
    15541 ttttctggcg aagcgttttg ctgtgaaaga agcggcggca aaagcgtttg gcaccgggat
    15601 ccgcaatggt ctggcgttta atcaatttga agtattcaat gatgagctcg gcaaaccacg
    15661 gctacggcta tggggtgagg cattaaaact ggcggaaaag ctcggcgttg taaatatgca
    15721 tgtaacgctg gcagatgaac gccactatgc ttgtgccacg gtaattattg aaagttaaaa
    15781 aaataccgtt ctctttataa agtaaaaccc ccgaactggt aaggaacggg ggttttcact
    15841 ttacaacttt aattttgtgc agcacacgat tccaacagaa atgattatgg atcaatttcg
    15901 ttatcctgta caagcaactt gcaatctctg acataaaaaa cgcgagcctt tacgaagaaa
    15961 tcgacattga taaaaagatt aacgtgaccg ctttttgata agtacattaa aatcggtttc
    16021 gttattggca attttgctgt gctttacacc atgccactga attcccccat tgaaacgagt
    16081 ggtgtcgtca aagctctggt gtggagtgca gcactcgatt ccaataactc gcacgttcag
    16141 ttttggggtg atgtaagggc taatctgaac ggctgcattc cttgtttaag gaaaaacgaa
    16201 tgactgattg ccgatacctg attaaacgga ttatcaaaat catcattgct gttttacagc
    16261 tgatccttct gttcttataa cacaaggaaa cgtacttaag gtgcgtccgg tgaaccagtc
    16321 ggacgcgcct ttaataacta taaataagtg tctgggcaga tactatataa attaacttag
    16381 tgaataatt
//
LOCUS       DAZKGF010000116        15822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZKGF010000116 DAZKGF010000000
VERSION     DAZKGF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.116"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1863)
                     /gene="htpG"
                     /locus_tag="ACHOQV_003449"
     CDS             complement(<1..1863)
                     /gene="htpG"
                     /locus_tag="ACHOQV_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFP9631764.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQL"
     gene            complement(1973..2578)
                     /gene="recR"
                     /locus_tag="ACHOQV_003450"
     CDS             complement(1973..2578)
                     /gene="recR"
                     /locus_tag="ACHOQV_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP9631765.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(2578..2907)
                     /gene="ybaB"
                     /locus_tag="ACHOQV_003451"
     CDS             complement(2578..2907)
                     /gene="ybaB"
                     /locus_tag="ACHOQV_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP9631766.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(2960..4891)
                     /gene="dnaX"
                     /locus_tag="ACHOQV_003452"
     CDS             complement(2960..4891)
                     /gene="dnaX"
                     /locus_tag="ACHOQV_003452"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP9631767.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNSVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    complement(3563..3627)
                     /gene="dnaX"
                     /locus_tag="ACHOQV_003452"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(5020..5571)
                     /gene="apt"
                     /locus_tag="ACHOQV_003453"
     CDS             complement(5020..5571)
                     /gene="apt"
                     /locus_tag="ACHOQV_003453"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP9631768.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(5724..6101)
                     /gene="ybaN"
                     /locus_tag="ACHOQV_003454"
     CDS             complement(5724..6101)
                     /gene="ybaN"
                     /locus_tag="ACHOQV_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFP9631769.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            6171..6698
                     /gene="priC"
                     /locus_tag="ACHOQV_003455"
     CDS             6171..6698
                     /gene="priC"
                     /locus_tag="ACHOQV_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFP9631770.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            6712..6873
                     /gene="rsmS"
                     /locus_tag="ACHOQV_003456"
     CDS             6712..6873
                     /gene="rsmS"
                     /locus_tag="ACHOQV_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFP9631771.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(6926..7024)
                     /locus_tag="ACHOQV_003457"
                     /pseudo
     CDS             complement(6926..7024)
                     /locus_tag="ACHOQV_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7085..10447)
                     /gene="mscK"
                     /locus_tag="ACHOQV_003458"
     CDS             complement(7085..10447)
                     /gene="mscK"
                     /locus_tag="ACHOQV_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFP9631772.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLRDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(10575..11222)
                     /gene="acrR"
                     /locus_tag="ACHOQV_003459"
     CDS             complement(10575..11222)
                     /gene="acrR"
                     /locus_tag="ACHOQV_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFP9631773.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            11364..12557
                     /gene="acrA"
                     /locus_tag="ACHOQV_003460"
     CDS             11364..12557
                     /gene="acrA"
                     /locus_tag="ACHOQV_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFP9631774.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            12580..15729
                     /gene="acrB"
                     /locus_tag="ACHOQV_003461"
     CDS             12580..15729
                     /gene="acrB"
                     /locus_tag="ACHOQV_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFP9631775.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         3666 a         4197 c         4170 g         3789 t
ORIGIN      
        1 cagctggttc atacgacgaa taaacaggtt cggatcttcc agcgtgccgc gttctgccag
       61 cagcgcctga tccagcagca gttctaccca ctcgctgaac ttggcttcat cttcagtatc
      121 tgccgcacgt ttcaccagta cgtgatccgg gttcagttcg aagatgtatt tcacttctgg
      181 cactttctgg cccgccgcag cgaacagttt cgccatctga gtgctcattt cgtccgcgtc
      241 ggtcgaaacg atcgctggcg tatcggtcag acggtgagtc agacggacat ctttcacgcg
      301 ctcgccgagc agggctttca cacggtcgat aaacggagtc agtgctttct ccgcttcttt
      361 cgcgctctca tcaacttcgt cagccagttt ttccagcgac tcgtcaactt tagacacaga
      421 ctggaacggt ttaccgtcga actcagtcag atagttcatc atccactcat cgatgcggtc
      481 ggaaagcagc agaacttcga tgcctttctt acgcagcagt tccaggtgcg ggctgctctt
      541 cgctgccgcg tagctgtctg cagtgatgta gtagattttc tcctgccctt ctttcatgcg
      601 ggaaacgtag tcttccagag acacggtctg cgcagaagaa tcggtatggg tagacgcaaa
      661 ccgcagcagt ttggcgatcg cttcctggtt agcgaaatct tccgccggac cttctttcag
      721 taccaggcca aactgttgcc aaaaggtctg gtatttttcc gcgtcgtctt tcgccagttt
      781 ttccagcatt tgcagcacac gcttggtcag cgcattgcgc aggttacgcg ttaccgtgct
      841 gtcctggagg atttcacggg aaacgttcag cggcagatcg ctggagtcaa tcagaccacg
      901 cacgaagcgc agatagttcg gcatgaactg ttctgcgtcg tccatgatga acacacgctg
      961 aacatacagt ttcagaccgt gtttatgatc gcggttccac atatcccacg gtgcctgaga
     1021 cggaatgtac agcaggctgg tgtactcctg cttaccttca acgcggttgt ggctccaggt
     1081 cagcggatcg ttaaagtcgt gggcgatgtg tttgtagaac tctttgtact cttcatcggt
     1141 gatttccgac ttgttacgag tccacagcgc ctgcgctttg ttgattttct cccaggagat
     1201 aacggtttcg ccgtctttct cttcgcgttt ttcgatctct accggcagcg cgatatggtc
     1261 ggaatatttg ctgatgatgg aacgcacacg ccagtcatcg aggaactcgt cttcgccttc
     1321 acgcagatgc agggtgattt cagtaccacg atcttctttg gtgatgtcgg caacagtgta
     1381 ttcaccttca ccagccgatt cccagaagac gccattttct ggtttttcgc ctgctgcgcg
     1441 agtacgcacg gtcactttgt cagccacgat aaacgcagag tagaaaccaa caccaaactg
     1501 accgatcagc tggctgtctt tcgcctggtc agaacccagg gattcgagga atgatttggt
     1561 gccggattta gcgatagtcc ccagatggtc aatcacttcg tcgcgggtca tccccacgcc
     1621 gttatcggag atggtcagcg tgcgcttgtc tttatcgaaa gagacacgaa cgcgtagttc
     1681 accatcacct tcgtacaggt ccgggttaga aagcgcgcgg aaacgcagct tgtccgccgc
     1741 atcggaggcg ttagagataa gctcacgcag gaagatttct ttattggaat agagagaatg
     1801 gatcatcagg tgcagaagct gtttcacttc tgactgaaaa ccacgagttt cttgtccttt
     1861 catgtaggtc tacctcaata atgccatttt aaggttaaaa acaggatgag ggagagatgg
     1921 ggacaaggga gaataatttc aagcgagagc aggtgatcct gctctcgttt gcttaaaaac
     1981 gaatcttatg acgcccggca agggaatgtg acagcgtggt gccatcgacc atttccagtt
     2041 cgccgccaac cggtacgcca tgagcgattc ggctggcttc cacgtcatat tgcgcacaaa
     2101 gctcggcaat gtagttagcg gtagcttcac cttcaaccgt ggggttggtg gcgaggatca
     2161 cttcagtgat tttttcctct gccagacgct gttccagacg atcaagcccg atatcatcag
     2221 gaccgatgcc gtccagcggt gacaggtgtc ccatcaacac aaaataacga cctgaaaact
     2281 gccccgtctg ctcaatggcg taaatgtccg ccggactctc caccacgcag atttgaccgt
     2341 tttcctgacg acgtggattc gaacagatgt tacagacttc ctgttcggtg aaggtgcggc
     2401 aatcggcgca gtggccgatt tccgacatcg cccgggtgag cgcctgcgcc agacgcatcc
     2461 cgccgctacg atcgcgctga agcagcgtga acgccatacg ctgcgccgac ttcgggccaa
     2521 cgcccggcag acagcgcagt gcttccataa gctgtgttaa cagcgggctg gtttgcatca
     2581 gaacggcatc ttaaagccag gcggcagctg cattccggag gatacagagg ccattttttc
     2641 tttctgcgtt tcttcaatac gacgtgctgc gtcgttgaat gctgcagcca ccaggtcttc
     2701 cagcatctct ttgtcgtctt ccagcaggct cgggtcgatc tctacgcgac ggcagttgtg
     2761 tgcaccgttg atggtcactt ttaccagacc tgcgccagat tcgccggtga cttccagctg
     2821 cgcgatctct tcctgcattt tctgcatttt ttcttgcatc tgctgggctt gcttcatcag
     2881 gttacccaga ccgcctttac caaacatagg tttctctctc aatcacgtta aggatgacga
     2941 acgtaagctg tgcttacgat caaatggggc ggatactttc ttcatccagc tccgcatcga
     3001 agaatcgacg cagggtctga atattattat ccgcaataat ggactcgcgc gcctgcgcaa
     3061 gtttttcttc gtatatcgcc tgacgccatt ccagcggcgt acgcaccgcg ggattatcat
     3121 cttcaacgat agtcagttca accgttgaac cttttaacgt gctcaacgct tcagccagtt
     3181 tttgctgtgc gccgcggttg ttcaaatgcc gctgagagga gcgcaaatgc agacatactg
     3241 agttgtcgct ctcctctttc caggcattta acgccacctg ttcgaccagt tttggtagcg
     3301 aaagttgact cacctgagcc gcccacgggt cgcgctcaat ggcttctgcc gccagcttcg
     3361 ccgccagttc cggcgttttt tcatgttcca gcgctttttt cagcgccttc ggcgtggcga
     3421 ccacttcttt ttgctgcatc actggagtgg tcgccttcca gcgatacgct tcttttttgg
     3481 ctggcgcttt ttccagcgcc gatggcaccg ggcgcgcctg aacgcgatcg gtgaccgaag
     3541 ccagtctttc cagcgcagcg ttattcaccg gccgcgcgcg ggtagcggct gccggttcac
     3601 tcttttttgc tttggttgct ccctgcacgc gctgcaactg ctggcgcgcc gccagcacct
     3661 ggctggtggt ttccgggagc ggtacagtcg gtgcctgctg cggcgctgat tgcggttgcg
     3721 gcggcacctg tgttggcgtc attactgccg ttggcgcaac gggcgcaaag gactggcgtg
     3781 gcacttctgg ctcaggcaga ggcatacgcg gatggaacgc cagcgcgcgc agcagcgtca
     3841 tctcaacacc catacggcgg tctggcgcat acggtaattc tttgcgacca atcaacagcg
     3901 tctgatagta aagctgaata tccgtcggcg gtatggtgcg cgccagttca cgcatccgca
     3961 gctcaatggc ggccatgtcg ttgccaagag cagcaggcga aagttgtacc atcgcaatac
     4021 gatgcaacag accgagcatt tccaccagca acgcttccca ctcgatacca cgggcagcag
     4081 cttcattaat tagcgccatt acgcgctcgc cgttggcctc gaccatcgct tcaaccagcg
     4141 ataacgcctg atcgtcgtca agcgtaccca gcatcgcact gaccgcctgg gttgaaacct
     4201 ggccgtcacc gctggcaatc gcctggtcgg tcagacttaa agcatctcgc aggctgcctt
     4261 cagcggcgcg tgccaacaat tgcagcgccc gcggctcgtg agcgatatgt tcttcgttga
     4321 ggatgtgctc aagctgatgg cgaatttgct cgacatccag cgccttgaga tgaaattgca
     4381 gacagcgtga caaaatcgtc accggcaatt tctgcggatc ggtcgtcgcc agcaaaaact
     4441 taacgtgctc tggcggctct tcaagggttt ttaacagtgc gttaaagctg tgacgcgaca
     4501 gcatatgcac ttcgtcgatg agataaactt tgaaacgacc acgcgccgga gcgtactgaa
     4561 cgttatccag caggtcgcgg gtatcttcga ctttagtgcg cgaggcggcg tcgatttcaa
     4621 tcagatcgac aaagcgcccc tgctcgattt cacgacagtt atcgcacacg ccgcacggcg
     4681 tcgcggtaat gccggtttcg cagtttagcc ccttcgccag cagtcgggcg atagaggttt
     4741 ttccgacgcc acgggtaccg gaaaaaagat aagcatgatg aatacgccct aacgacaagc
     4801 cgttcgccag tgcggtcagc acatgttcct ggccgacgac gtcagcaaag gtttgtgggc
     4861 gccattttcg ggctaagacc tgataactca ttggcaggct ctgaaacgct ggaaggtgga
     4921 ttcacgaagg ggtaatgcta acacagcccc gtcagaacgg cgaggctgaa tgcacatctt
     4981 gtgctgtccg tagcgtgggc agcacaagac tggcgataat taatggcccg ggaacgggac
     5041 aaggctgtag ctggtaatgc cctgtttttc gagacgctgt tcgccgccga gatcgaacag
     5101 gttgataatg aacgcagcgt cagccacttc accacccaga cgacggatca gtttaacggt
     5161 cgcttcgata gtgccgccgg ttgccagcag atcgtccacc accagaactt tgtcgcccgg
     5221 tttgatggca tcaacgtgga tttccagctg atcggtgccg tattccaggt cgtaagtttc
     5281 actgatggtt tcacgcggca gtttgcccgg tttacggacc ggtacaaagc caacgcccag
     5341 acccagagct accggagcgc caaacaagaa gccacgcgct tcggtgccga caactttggt
     5401 aatgcccgca tttttgtaac gctcaaccag caagtcgatg ctgagagcgt aagctttcgg
     5461 gtcttccagt aagctggtga catcacggaa aagaatgccg ggttttgggt agtcctgaat
     5521 gcttttgatg ctatttttga gatactcaag ctgctgtgca gtcgcggtca taagtgtatg
     5581 cctgcttgtt acggtggtac tcacggcgcg ttttaaacgt atcaaaagta acagttgttt
     5641 aacctttgac cgcctgcgcc tgtgctcgaa aacgccagaa tgtactggct gtacgcaata
     5701 attgcaactg cgattgttgt gcttcagtgc ttttcttgct tttcatcaat caccggaatt
     5761 cgccacatat aaaaaagcaa acaggcgaga attaccagca acatgatgcg cacccatggc
     5821 atctggacga accacagaga aatggcaaac gtgagcaaaa taagcaaaat cgcccgcggt
     5881 ttgacgccgc gcggcatcgc atgatgtttc tgccagaaac gtagatagct gccaaaccat
     5941 gagcggtaca gcaaccaggc gtgaaagcgc ggggaagaac gggcaaagca ccaggccgcc
     6001 agcaggataa acggcgtcgt cggtaatacc ggtaatacca cgcccagcgt acccagcact
     6061 accgccagcc agccaatgat gattaaaatg attcgttgca tattgagtgt tgtcagctta
     6121 cagagttgct actttagcat aacaattatc attttcattg aggtcttatc gtgaaaaccg
     6181 ccctgctgct ggaaaaactg gaaggacagc tcgctacgct gcgtcagcgt tgtgccccgg
     6241 tagcgcaatt tgccacgcta agcgcccgtt ttgacaggca tctttttcag actcgtgcga
     6301 caacactgca ggcttgtctc gatgaggcgg gcgataatct ggctgcgctt cgtcatgcgg
     6361 ttgagcagca acagctgccg caagtcgcct ggctggcaga acatctggcg gcgcaactgg
     6421 aggctatagc gcgtgaagcc acagcctggt cgctacgcga gtgggatagc gcaccaccga
     6481 aaattgcccg ctggcagcgt aaacgtattc agcatcagga ttttgagcgg cggctacgtg
     6541 agatggttgc cgaacgtaga gcccgtctgg cacgagcgac cgatctcgtg gaacagcaga
     6601 cgctgcatcg tgaagtggaa gcctatgaag cgcgcctggc acgctgccgc catgcgctgg
     6661 aaaaaatcga aaacaggtta gcgcgtttaa cccgctagca atggagagag tatgtcactg
     6721 gaaaatgccc ctgacgatgt caaactggcc gtcgatttga ttgtgctact ggaagaaaat
     6781 cagatcccag cccgcaccgt gctgcgcgcg ctggatattg taaaacgcga ttatgaaaag
     6841 aaattaacgc gcgatgatga ggcggaaaag tgagaaataa atggggccgc taaatgtccc
     6901 ctaagattgt gggcaaacgc aaaactgcct gatgcgctac gcttatcagg cctacaccgc
     6961 tcctgcaata tattgaattc ttgcggtttc gtaggccgga taaggcggtc acgccgcatc
     7021 cggcatgaac aacgcgcact ttgttagtaa tctcggggca actaaatgtc gccccctttg
     7081 gttattaccc taccgctggc gtcggatcgt cgcctttgta gtcgcgtttg acttccgtca
     7141 cctcatcgcc cttctcgttg tgcagatgca cttcaagctg gttaaaggca atgttgatgt
     7201 cgttttcacg gcacaactga tcgatagtac ggttcagctc atcgacagta cggctacggt
     7261 cacgcagttc acgcacatac agacgcagct catgatccaa cgtgctggca ccaaatgccg
     7321 taaagaagac ttccggcatt ggttcgtgca tcacccttgg gtgctcagtc gccgccttca
     7381 gcaacacttt acgcactttt tccagatcgg agccataagc tacgccgaga cggatcacca
     7441 gacgcgtagt cgtgtcagtc aacgaccagt tgatcagacg ctcggtaaca aacgctttgt
     7501 tcgggatgat cacttctttg cgatcgaaat cggtaatcgt tgtcgcacga atacggatct
     7561 tacttaccgt ccccgagaag ctaccaatgg ttaccgtgtc gcctatgcgc accggacgtt
     7621 cgaacagaat gatcaaaccg gaaacgaagt taccgaagat ctcttgtaaa ccaaaaccaa
     7681 gacctacgga taatgctgcc gccagccact ggagtttatc ccacgagacg cccagcgatc
     7741 cgaacaccgt catcgcacca acggcaataa tgatgtagtt aaggatggta gtaatggcat
     7801 acgacgcgcc ctggcgcatg ttcaggcgcg agagcaccaa cacttccagc aaaccaggca
     7861 ggttgcgaat caacgcccag gccaccattg aggcgataat cgcaaacaac agactgccca
     7921 tggtgacgtt tttcaccacc gcagcaccag cttcagtgcc gttgtaatgc cagagcgtga
     7981 tgctgtccag atagctgaac acggtgatca aatcggacca aattgcccag aacatgacac
     8041 cgaacagcgc aaacatcagc aacatggtaa tacgcagcgt ctgctggtta acctgctcca
     8101 gtgcaatggt gggttcttcc ggcggttcag caccttctgc gccctctttc accagattct
     8161 gccgacgcgc cagcgcacga cgccaggcga tacgccgcgc cgctacgctt aagccgcgca
     8221 ataccgtctg gtacagcagg ttccagatga tcaccaaata aacggtttca atccaacgcc
     8281 cggagaggcg cagcgtggtg tagaaataac ccgtggcggt cagcaccatc aacgcaatcg
     8341 ggataatcga cagtacggta atggtgacca gtcgcatggt gtgcgactct ttatcacgcc
     8401 agctttcgcg gcacatcggc cacaccagga aggcaatcag cagcaagttg aagaaaatca
     8461 tcgcctgccc cagcacatca tccatcagat gcagcgggga aagttctgcc accacagacc
     8521 agaaatggat aggcagcaac gcgagactga tgcggacaat ttgccgacgc cagtggctgg
     8581 tctgctgttc cggcatacca aagtgacgca cggcaacgcc gtttttctcc aacactttcc
     8641 agcacaggcc aaacaccagc cagaaaatcg ccagtttttt actgaacgac catagcagtt
     8701 cgctgatgtt gagctgcatg gtcaacagaa tcaggccaac cgcgagaata atcaggcaca
     8761 ccggcagcgc acggatcagg tcgataagga tcgcttttgg tgtgttgagc tggctgtcgt
     8821 tacgcaggga acccacagcg gaagccagtt tttgttgata cgctttcagc cagcccagac
     8881 gccagtggat cagcccggca atcaacagca gcggcaaacc agcgaggaaa gcgataaaaa
     8941 cggcaggcca ggctttttcc cagttcaccg tgattttcat cgacttaaat tcatctctca
     9001 ggctttgcgg gaacgctttg atccagtccc agtccattgg acggttacta ttcacccaaa
     9061 agatttgctg agtcaggatg gatttcaggt ttttcgacac actcattaac tgctgctggt
     9121 tgatttgcag gttaatggcc atcatcagct ggttacccaa ctgtttgtta agctgatcca
     9181 gcaattcgcg acgcatatca accacttgca ataacgcatc gtgaacttcg ctgttgactt
     9241 cgttggtgtg accttcttcc agtttgttga cgaacgcatc gctctggaag agtgcatcac
     9301 gctgctggtt aacttcaaac tgttcgagac gcaaatccgc gatgcggttg gtcatgtttt
     9361 ccagttcatc cgccgagggc agcgtttgtt gttgctggta aaggatacga gacaacagca
     9421 ggctgccctt caggacggca atctgctctt taatattgcg ttccgattgc agcgcccgct
     9481 ccagccagtt tttgacttta atgttttgct gcatcaactg attaccgttt tcagtcgcgg
     9541 taatcagacg ctgacttaac tgctggttaa tttccagttc ctgcttcacc agcggattag
     9601 cctgaatacg cgcggcttca tccggggaga cggcttcctg cgccgttttt tcggttaaag
     9661 tcaagcgctt gctgtttact gcttcttgca acagctgcaa ctggtgctcc agacgggcgc
     9721 tgttcgccgt cacgtaatcc cgttgctttt gcaaggtatc ctgtaagact gtgttccctt
     9781 ccaggctttt acgctgctgg tcaatctcgg cattcagcaa agcctgctga gcctgcatta
     9841 acactttctg gctgggacgt aaggcagtct cgccgacatc agtcccatcc agacgactgc
     9901 gaatttgttg cagctgctgc gaagcgttat acatcgcatt ttgcacgcgt tcgggctgcg
     9961 tctgtaacga aaccagctgg ctgttataag acgccagatc gttttgtgcg ttttgcaaat
    10021 cgtccagcgc ctgggcaacg cgagtttcca gctggcgcaa cgaaagcgta ctcagaattt
    10081 tgcgagtttc ttcgtcatta tcgacatcgc taagtgcagt taacgccgcg gtcgcctggc
    10141 gcattttttc cggcgcttca gcaacctttt gccgtagctg aactgtctct tcttttacgc
    10201 gatcgatttt atcgagggta gctaatgtat ctgtcagatc ctgctgcacc agtttgtcct
    10261 gagcagaaag atctttttgt ttattcagtg agtcaagttg tgcctgcagg tccgcttttg
    10321 tcggcagatc accattcgat gacgcccgcg caaacgccgt gttctggcat aacaagacaa
    10381 aaacaaaagc aataaatgct gaaaaaacaa aatgccgtga tcgtttgtaa tactggaaca
    10441 tagtcatgat gaatgaaggt ttctgaacct gaagaacgac ctgaaaaagt caaaccgcaa
    10501 gaatatcacg acgcagtgaa ccagaatagc aacgacgaaa atgtccagga aaaatcctgg
    10561 agtcagattc aaggttattc gttagtggca ggattacgaa gcgtggggca caggagatac
    10621 atctccagta agatggcgac gtaatcgcgg gcttcttttt taagatcaaa agattgcggg
    10681 gcaaagagcc agttttccat caggccggaa atatagccgc gcataataat tgctgcccga
    10741 cgcgtcatta aatccgcagg caacattttc gcttcaatac aatgttttaa cgtttgttct
    10801 atacggtcat aactttccag acagagatta cgttgtgcct gttgcacaac agccatttct
    10861 ccgacaaatt cgcatttgtg gaatataatc tccatcaata atcgacgccg ttcttctgtc
    10921 accgtggatt caagaacatg aattaatatc tctcttaata ctgagagtgg atcgccaggg
    10981 aattttgcct gatactcaag ctcgagttca ccaatattgg attctgacag ttcccagatc
    11041 tcactgaaca aatccgactt gtctttaaaa tgccagtaga ttgcaccgcg cgtaacgcca
    11101 gctgcttttg caatctcgcc cagcgaggtg gatgataccc cctgctgtga gaaaagacgt
    11161 agagccacat cgaggatgtg ttggcgcgtt tcttgcgctt cttgtttggt ttttcgtgcc
    11221 atatgttcgt gaatttacag gcgttagatt tacatacatt tgtgaatgta tgtaccatag
    11281 cacgacgata atataaacgc agcaatgggt ttattaactt ttgaccattg accaatttga
    11341 aatcggacac tcgaggttta catatgaaca aaaacagagg gtttacgcct ctggcggtcg
    11401 ttctgatgct ctcaggcagc ttagccctaa caggatgtga cgacaaacag gcccaacaag
    11461 gtggccagca gatgcccgcc gttggcgtag taacagtcaa aactgaacct ctgcagatca
    11521 caaccgagct tccgggtcgc accagtgcct accggatcgc agaagttcgt cctcaagtta
    11581 gcgggattat cctgaagcgt aatttcaaag aaggtagcga catcgaagca ggtgtctctc
    11641 tctatcagat tgatcctgcg acctatcagg cggcatacga cagtgcgaaa ggtgatttgg
    11701 cgaaagccca ggctgcagcc aatatcgcgc aattgacggt gaatcgttat cagaaactgc
    11761 tcggtactca gtacatcagt aaacaagagt acgatcaggc tctggctgat gcgcaacagg
    11821 cgaatgctgc ggtcactgcg gcgaaagctg ccgttgaaac tgcgcggatc aatctggctt
    11881 acaccaaagt cacctcgccg attagcggtc gcattggtaa gtcgaacgtg acggaaggcg
    11941 cattggtaca gaacggtcag gcgactgcgc tggcaaccgt gcagcaactt gatccgatct
    12001 acgttgatgt gacccagtcc agcaacgact tcctgcgcct gaaacaggaa ctggcgaatg
    12061 gcacgttgaa acaagaaaac ggcaaagcca aagtgtcgct gatcaccagt gacggcatta
    12121 agttcccgca ggacggtacg ctggaattct ctgacgttac cgttgatcag accaccgggt
    12181 ctatcaccct acgcgctatc ttcccgaacc cggatcacac tctgctgccg ggtatgttcg
    12241 tgcgggcacg tctggaagaa gggcttaatc caaacgctat tttagtcccg caacagggcg
    12301 taacccgtac gccgcgtggc gatgccaccg tactggtggt tggcgcggat gacaaagtgg
    12361 aaacccgtcc gatcgttgca agccaggcta tcggcgataa gtggctggtg acagaaggtc
    12421 tgaaagcagg cgatcgcgta gtaataagtg ggctgcagaa agtgcgtcct ggtgtccagg
    12481 taaaagcaca agaagttacc gctgataata accagcaagc cgcaagcggt gctcagcctg
    12541 aacagtccaa gtcttaactt aaacaggagc cgttaagaca tgcctaattt ctttatcgat
    12601 cgcccgattt ttgcgtgggt gatcgccatt atcatcatgt tggcaggggg gctggcgatc
    12661 ctcaaactgc cggtggcgca atatcctacg attgcaccgc cggcagtaac gatctccgcc
    12721 tcctaccctg gcgctgatgc gaaaacagtg caggacacgg tgacacaggt tatcgaacag
    12781 aatatgaacg gtatcgataa cctgatgtac atgtcctcta acagtgactc cacgggtacc
    12841 gtgcagatca ccctgacctt tgagtctggt actgatgcgg atatcgcgca ggttcaggtg
    12901 cagaacaaac tgcagctggc gatgccgttg ctgccgcaag aagtacagca gcaaggggtg
    12961 agcgttgaga aatcatccag cagcttcctg atggttgtcg gcgttatcaa caccgatggc
    13021 accatgacgc aggaggatat ctctgactac gtggcagcga atatgaaaga tgccatcagc
    13081 cgtacgtcgg gcgtgggtga cgttcagttg ttcggttcac agtatgcgat gcgtatctgg
    13141 atgaacccga atgaactgaa caaattccag ctaacgccgg ttgatgtcat taccgccatc
    13201 aaagcgcaga acgcccaggt tgcggcgggt cagctcggtg gtacgccgcc ggtgaaaggc
    13261 caacagctta acgcctctat tattgctcag acgcgtctga cctctactga agagttcggc
    13321 aaaatcctgc tgaaagtgaa tcaggatggt tcccgcgtgc tgctgcgtga tgtggcgaaa
    13381 attgagctgg gtggtgagaa ctacgacatc atcgcagagt ttaacggcca accggcttcc
    13441 ggtctgggga tcaagctggc gaccggtgca aacgcgctgg ataccgctgc ggcaatccgt
    13501 gctgaactgg cgaagatgga accgttcttc ccgtcgggtc tgaaaattgt ttacccgtac
    13561 gacaccacgc cgttcgtgaa aatctcgatt cacgaagtgg ttaaaacgct agtcgaagcg
    13621 atcatcctcg tgttcctggt tatgtatctg ttcctgcaga acttccgcgc gacgttgatt
    13681 ccgaccattg ccgtaccggt ggtattgctg gggacctttg ccgtccttgc cgcctttggc
    13741 ttctcgataa acacgctaac aatgttcggg atggtgctcg ccatcggcct gttggtggat
    13801 gacgccatcg ttgtggtaga aaacgttgag cgtgttatgg cggaagaagg tttgccgcca
    13861 aaagaagcta cccgtaagtc gatggggcag attcagggcg ctctggtcgg tatcgcgatg
    13921 gtactgtcgg cggtattcgt accgatggcc ttctttggcg gttctactgg tgctatctat
    13981 cgtcagttct ctattaccat tgtttcagca atggcgctgt cggtactggt ggcgttgatc
    14041 ctgactccgg ctctttgtgc caccatgctg aaaccgattg ccaaaggcga tcacggggaa
    14101 ggtaaaaaag gcttcttcgg ctggtttaac cgcatgttcg agaagagcac gcaccactac
    14161 accgacagcg taggcggtat tctgcgcagt acagggcgtt acctggtgct gtatctgatc
    14221 atcgtggtcg gcatggccta tctgttcgtg cgtctgccaa gctccttctt gccagatgaa
    14281 gaccagggcg tatttatgac catggttcag ctgccagcag gtgcaacgca ggaacgtacg
    14341 cagaaagtgc tcaatgaggt aacgcattac tatctgacca aagaaaagaa caacgttgag
    14401 tcggtgttcg ccgttaacgg cttcggcttt gcgggacgtg gtcagaatac aggtattgcg
    14461 ttcgtttcct tgaaggactg ggccgatcgt ccgggcgaag aaaacaaagt tgaagcgatt
    14521 accatgcgtg caacacgtgc tttctcgcaa atcaaagatg cgatggtttt cgcctttaac
    14581 ctgcccgcaa tcgtggaact gggtaccgca accggctttg actttgagct gattgaccag
    14641 gctggccttg gtcacgaaaa actgactcag gcgcgtaacc agttgcttgc agaagcagcg
    14701 aagcaccctg atatgttgac cagcgtacgt ccaaacggtc tggaagatac cccgcagttt
    14761 aagattgata tcgaccagga aaaagcgcag gcgctgggtg tttctatcaa cgacattaac
    14821 accactctgg gcgctgcatg gggcggtagc tatgtgaacg actttatcga ccgcggtcgt
    14881 gtgaagaaag tttacgtcat gtcagaagcg aaataccgta tgctgccgga tgatatcggc
    14941 gactggtatg ttcgtgctgc tgatggtcag atggtgccgt tctcggcgtt ctcctcttct
    15001 cgttgggagt acggttcgcc gcgtctggaa cgttacaacg gcctgccatc tatggaaatc
    15061 ttaggccagg cggcaccggg taaaagtacc ggtgaagcaa tggagctgat ggaacaactg
    15121 gcgagcaaac tgcctaccgg tgttggctat gactggacgg gaatgtccta tcaggaacgt
    15181 ctctccggca accaggcgcc ttcactgtac gcgatttcgt tgattgtcgt gttcctgtgt
    15241 ctggctgcgc tgtacgagag ctggtcgatt ccgttctccg ttatgctggt cgttccgctg
    15301 ggggttatcg gtgcgttgct ggctgccacc ttccgtggcc tgaccaatga cgtttacttc
    15361 caggtaggcc tgctcacaac cattggtttg tcggcgaaga acgcgatact tatcgtcgaa
    15421 ttcgccaaag acttgatgga taaagaaggt aaaggtctga ttgaagcgac gcttgatgcg
    15481 gtgcggatgc gtttacgccc aatcctgatg acctcgttgg cgtttatcct cggcgttatg
    15541 ccgctggtta tcagtactgg tgctggttcc ggcgcgcaga acgcagtagg taccggtgta
    15601 atgggcggga tggtgaccgc aacggtactg gcaatcttct tcgttccggt attctttgtg
    15661 gtggttcgcc gccgctttag ccgcaagaat gaagatatcg agcacagcca tactgtcgat
    15721 catcattgat acaacgtgta atcactaagg ccgcgcaagc ggcctttttt atgcataacc
    15781 gacgaacatt aaggagtcat tgaaccacca actcaggatc tc
//
LOCUS       DAZKGF010000117        15476 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZKGF010000117 DAZKGF010000000
VERSION     DAZKGF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.117"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            99..743
                     /gene="adk"
                     /locus_tag="ACHOQV_003462"
     CDS             99..743
                     /gene="adk"
                     /locus_tag="ACHOQV_003462"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP9631776.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            875..1837
                     /gene="hemH"
                     /locus_tag="ACHOQV_003463"
     CDS             875..1837
                     /gene="hemH"
                     /locus_tag="ACHOQV_003463"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFP9631777.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(1834..2793)
                     /gene="aes"
                     /locus_tag="ACHOQV_003464"
     CDS             complement(1834..2793)
                     /gene="aes"
                     /locus_tag="ACHOQV_003464"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFP9631778.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FTGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            2945..4249
                     /gene="gsk"
                     /locus_tag="ACHOQV_003465"
     CDS             2945..4249
                     /gene="gsk"
                     /locus_tag="ACHOQV_003465"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFP9631779.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(4333..6009)
                     /gene="ybaL"
                     /locus_tag="ACHOQV_003466"
     CDS             complement(4333..6009)
                     /gene="ybaL"
                     /locus_tag="ACHOQV_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFP9631780.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(6247..7467)
                     /gene="fsr"
                     /locus_tag="ACHOQV_003467"
     CDS             complement(6247..7467)
                     /gene="fsr"
                     /locus_tag="ACHOQV_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFP9631781.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            7685..9337
                     /gene="ushA"
                     /locus_tag="ACHOQV_003468"
     CDS             7685..9337
                     /gene="ushA"
                     /locus_tag="ACHOQV_003468"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9631782.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLIMAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNNLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKAGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(9374..9853)
                     /gene="ybaK"
                     /locus_tag="ACHOQV_003469"
     CDS             complement(9374..9853)
                     /gene="ybaK"
                     /locus_tag="ACHOQV_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFP9631783.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(10057..10851)
                     /locus_tag="ACHOQV_003470"
     CDS             complement(10057..10851)
                     /locus_tag="ACHOQV_003470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFP9631784.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEKRISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLCAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            10984..11286
                     /locus_tag="ACHOQV_003471"
     CDS             10984..11286
                     /locus_tag="ACHOQV_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9631785.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            11322..11663
                     /locus_tag="ACHOQV_003472"
     CDS             11322..11663
                     /locus_tag="ACHOQV_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP9631786.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(11721..14225)
                     /gene="copA"
                     /locus_tag="ACHOQV_003473"
     CDS             complement(11721..14225)
                     /gene="copA"
                     /locus_tag="ACHOQV_003473"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFP9631787.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSVTEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTNEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            14488..15420
                     /gene="glsA"
                     /locus_tag="ACHOQV_003474"
     CDS             14488..15420
                     /gene="glsA"
                     /locus_tag="ACHOQV_003474"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP9631788.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT         3922 a         4087 c         4011 g         3456 t
ORIGIN      
        1 gtttcagccc caggtgcctt tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt
       61 tttcaaaaaa ttcgacacat tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg
      121 ctccgggcgc ggggaaaggg actcaggctc agttcatcat ggagaaatat ggtattccgc
      181 aaatctccac tggcgatatg ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac
      241 aagcaaaaga cattatggat gctggcaaac tggtcaccga cgaactggtg atcgcgctgg
      301 ttaaagagcg cattgctcag gaagactgcc gcaacggttt cctgttggac ggcttcccgc
      361 gtaccattcc gcaggcagac gcgatgaaag aagcgggcat caatgttgat tacgttctgg
      421 aattcgacgt accggacgaa ctgattgttg accgtatcgt aggccgccgc gttcacgcgc
      481 cgtctggtcg tgtttatcac gttaaattca atccgccgaa agtagaaggc aaagacgacg
      541 ttaccggtga agagctgact acccgtaaag atgatcagga agagaccgta cgtaaacgtc
      601 tggttgaata ccatcagatg actgcaccgc tgatcggcta ctactctaaa gaagcggaag
      661 cgggtaacac caaatacgcg aaagttgacg gcaccaagcc ggtggctgaa gttcgcgcag
      721 ctctggaaaa aattctcggc taatcaagcc gcagcagatc tgctttcggg caggtctgct
      781 catcctcctc aggcaatacc tctcacagca attagttctt cttcttcact tttccgctac
      841 aattatcaac aagttgaatc gataagaggc ggtaatgcgt cagacaaaaa ccggtatcct
      901 gctggcaaac ctgggtacgc ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa
      961 acaattttta agcgacagac gtgtggttga tacctcacgg ttgttatggt ggccattgct
     1021 gcgcggcgtg attttgccac tgcgctcgcc gcgtgtggcg aagctgtatg cctctgtctg
     1081 gatggaaggt ggctcgccgc tgatggttta cagccgccag caacagcagg cgctggcaca
     1141 acgtttaccg gagacgcccg tagcgctggg aatgagctac ggctcgccat cactggaaag
     1201 cgccgtagat gaactactgg cagagcatgt agatcatatt gtggtgctgc cgctttatcc
     1261 gcaattctcc tgttcaacgg tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg
     1321 caaacgtagc attccgggga tatcgtttat tcgtgattac gccgataacc acgattatat
     1381 taatgcactg gcgaacagcg tacgtgcttc ttttgccaaa catggcgaac cggatctgct
     1441 gttgctctct tatcatggca ttccccagcg ttatgcagat gaaggcgatg attacccgca
     1501 acgttgtcgc acaacgactc gcgaactggc ttccgcactg gggatggcac cggaaaaagt
     1561 gatgatgacc tttcagtcgc gctttgggcg ggaaccctgg ctgatgcctt ataccgacga
     1621 aacgctgaaa atgctcggag aaaaaggtgt aggtcatatt caggtgatgt gcccgggctt
     1681 tgctgcggat tgtctggaga cgctggaaga gattgccgag caaaaccgtg aggtcttcct
     1741 cggtgcgggc gggaaaaaat acgaatatat tccggcactt aatgccacgc cggaacatat
     1801 tgaaatgatg gctaatcttg ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc
     1861 gccgtcgcga agagcctcgt cggcggtttt catcatccgt gaataatgca aaaaggcgtg
     1921 cagcgtgcct gggtagagtt tgaactcaca gggctgctga tgcgccgcta acgtctggta
     1981 aagcagacgg ctgtcatcca gcagcggatc gaactccgcc ccggcaataa aacagggcgg
     2041 aacttcgcga gtgagatcat tattaaacag gcagtaatac ggcgactcgc ggtccgcgtc
     2101 gttgcttaaa tacgcctctt catacatctg taaatcctgt tgcgttaagc catcccagac
     2161 accgcccaac agacgacgag tcacggaatc ccgtaatccg taaagcccat accacagcaa
     2221 aacgcccgca actttaccgc aatcgatctg tttatcacgc aaccacaacg cactggcgag
     2281 cgccaacatg gcacctgcgg aatcaccggt aaagccaatg cgggacatat tgatttgata
     2341 atcttccgcc tgctggtgga aataacaaca ggcagccaca atttcctcta ttgcttgcgg
     2401 aaaacgcgct tcaggtgaaa gggtgtaatc aatgccaatc accgtacatt ggctgtagct
     2461 tgccagcagg cgcatgatgc gatcgtgggt atcaagattg ccgagaataa aaccgccgcc
     2521 atgcaaataa aatagcgtcg ccgggctatc tggctgcgga caaaagagac gtgtttccac
     2581 ctgcccatat tttgttggaa ccatgtaagc tctggttgcc atttctggag cgcccgcatt
     2641 ccagaatcgg cgctcaagcg tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg
     2701 ccagggcggt aaatccggct gaagagtatt cacaacggtc ttcatttcag cagaaataag
     2761 gtccagaacg ggtaatttgt tttccggctt catgcaaaaa ctcctttcaa attacgtcat
     2821 tgtaaggaac cactgccatg aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt
     2881 ctcgcgcgct cccgtcagat tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac
     2941 aaccatgaaa tttcccggca aacgtaaatc caaacattat tttcccgtaa acgcacgcga
     3001 tccgctgctt cagcaattcc agccagaaaa cgaaaccagc gctgcctggg tagtgggtat
     3061 cgatcaaacg ctggtcgata ttgaagcgaa agtggatgat gaatttattg agcgttatgg
     3121 attaagcgcc gggcattcac tggtgattga ggatgacgta gccgaagcgc tttatcagga
     3181 actaaaacag aaaaacctga ttacccatca gtttgcgggt ggcaccattg gtaacaccat
     3241 gcacaactac tcggtgctcg cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa
     3301 tattgaaatt ggcagctatg cctatcgtta cctgtgtaac acttccagcc gtaccgatct
     3361 taactatcta caaggcgtgg atggcccgat tggtcgttgc tttacgctga ttggcgagtc
     3421 cggggaacgt acctttgcta tcagccccgg ccacatgaac cagctgcggg ctgaaagtat
     3481 tccggaagat gtgattgccg gagcctcggc tctggttctc acctcttatc tggtgcgttg
     3541 caagccgggt gaacccatgc cggaagcaac catgaaagct attgagtacg cgaagaaata
     3601 taacgtaccg gtggtgctga cgctgggcac caagtttgtc attgccgaga atccgcagtg
     3661 gtggcagcaa ttcctcaaag accacgtctc tatccttgcg atgaacgaag atgaagccga
     3721 agcgttgacc ggagaaagcg atccgttgtt ggcatctgac aaggcgctgg actgggttga
     3781 tctggtgctg tgtaccgcag ggccaatcgg cttgtatatg gcgggcttta ccgaagacga
     3841 agcgaaacgt aaaacccagc atccgctgct gccgggtgct atagcggaat ttaaccagta
     3901 tgagtttagc cgcgccatgc gccacaagga ttgccagaat ccgctgcgtg tctattcgca
     3961 cattgcgccg tacatgggcg ggccggaaaa aatcatgaac actaatggag caggggatgg
     4021 cgcactggca gcgttgctgc atgacattac cgccaacagc taccaccgta gcaacgtacc
     4081 aaactccagc aaacataaat tcacctggtt aacttattca tcgttagcgc aggtgtgtaa
     4141 atatgctaac cgtgtgagct atcaggtact gaaccagcat tcacctcgtt taacgcgcgg
     4201 cttgccggag cgtgaagaca gcctggaaga gtcttactgg gatcgttaag ttatcgacca
     4261 gaccggacga cgatggcatc cgtaggccgg ataagatgcg tcagcatcgc atccggcaat
     4321 ggaatcaatg ttttaccccg tcaccacctc acccgccggt ggcgtttcca gcagttccag
     4381 catagtacga gcaatttcac gctcacccat cactacctga ttcgcaccac gttcggtgat
     4441 atacgccact tcatcgtcat aatgggcgcg ggcaataatc tcaatatccg gatttttcgc
     4501 gcgggcagat gccacaatct cgcccgcttc ataaccgttg ggaatcgtca ggatcagcca
     4561 ttttgcacat tccagatgcg ccagttgcat aatttcttcg ttcgccgcat tgcccaatac
     4621 tgcgcggacc ccgcgctctc gcagttcatc aacacgggtt cgtgacgtct caatcaccac
     4681 cagcggaata tcagaggcga gcaatttctc ccccagcagg ctgcctacac gaccgtaacc
     4741 taccagtagc gcatggttgc aaatatccac tgggatctgc ttctcttctt cgatagcctc
     4801 ttccagcgtc tgctcttcca gcgtttcggt cttcgccaga tatttctcca gcagcgcaaa
     4861 caggaccggg ttgagcataa tcgacaaaat cgcccctgcc agtaccaggt tctgcccggc
     4921 ttgcggcaat agatccagcg ccatgcccag tcccgccagg ataaacgcga actcaccaat
     4981 ttgcgccagg ctggcggcga tagttaatgc cgtacgttgg gagtgaccaa acagtcgcac
     5041 caggaaaaac gcggctaacg acttaccaaa cagaataatc gccagcgtcg ccagcactgc
     5101 cagcggttgc tgaatcagaa ttaacggatc aaacaacatc ccgacggaga caaaaaacag
     5161 caccgcaaac gcgtcgcgca atggcagcgt atcgtgggcg gcacggtgac tcagttcaga
     5221 ctcgttcagt accatcccgg caaagaacgc accgagtgca aaggagacat caaacagctc
     5281 caccgcacca aaggcaatcc ctaacgccag cgccagcacc gacagggtaa acagctcacg
     5341 ggaaccggtt gctgcgctac gtgccataat ccacggcacc agacggcgac ctaccagcat
     5401 cataatggcg ataaatgcga tcaccttgcc gatggtgatc cccatatcga cagcaagagt
     5461 ggcaaagccc acatcgcctt gttccatcat tcctgccact gcgggcagca acaccagcgt
     5521 cagaaccatg accaggtctt ccacaatcaa ccaaccgatg gcgatttgcc cacgctgact
     5581 gtcaattaat tgccgttctt caagtgcgcg cagtaacacc acggtactgg cggtggaaag
     5641 gcataagccg aacacgatac cggtcattaa cgaccagccc agcacggcgg agagcgccat
     5701 acccagcagc gtcgccacgg ctatctgggc gatcgcaccg ggaatggcga tggcctttac
     5761 cgccatcaaa tccttcagcg aaaagtgcag acccacgcca aacatcaaca gaatgacgcc
     5821 cagttcagcc agttccggcg caagcttggt atcggcaaca aagcccggag tgaatggtcc
     5881 tgccagcaca cccgctaaca gatatcccac cagaggagaa atacgtagtt tattggccag
     5941 catgccgagg ataaaggcga gcacaaggcc gccaacaatg gtggtgataa gcggggtggc
     6001 gtgatgcatt ccgtctcctt ttcctggtgg ttattgtcca tttttggccg ggaaaaccaa
     6061 aattacaggt aatagtttat gacaatttca ttgatgatgt ttatgaataa ttgttgaatt
     6121 ttgcagaaaa atggaattag ctgcaaaaaa agcacggatc ggaaaacaga aggcggttta
     6181 acagaggaaa ggcttatggc gactgtaaaa cgatgcagcc aaagtttgcc tttggctgca
     6241 atgaaatcag tctttatgcc ggttatcagg caggaatatg gtcaacatcc ccaatagtgg
     6301 caggaaagca cagattttat agactaactc gatgctggtg tgatcggcga taagccccag
     6361 aactgccgct cccagacctc ccatgccaaa agcaaaaccg aaaaagagtc cagaaaccat
     6421 accgatacgt cccggaagta gctcctgagc gtagaccaga atggcagaga atgccgaagc
     6481 gaggataaat ccaataatca ccgttaaaac ccccgtccag tgcaggctgg cgtagggtaa
     6541 aatcagcgta aacggcgcaa cgccgaggat agagccccaa atcacatatt tccgtccaat
     6601 tttatcccct acaggcccgc caatcaccgt acctgccgca acggcaaaca ggaaggcaaa
     6661 caaatgaagc tgagcattct ggatagataa tccgaatttt tgcatcagat aaaaggtgta
     6721 atagctgctg atgctcgcca tatagaaata tttcgagaaa atgaggatta acagaatgct
     6781 gaccgccaga acaactttat tgcgcggcag tggattgata atcgtcgctt tgggttttcc
     6841 tttattcatt cggtgctgtg ccgagtacca acggctgatt tgcgccaaca ccacgatcgc
     6901 cagcagtgcc gcaagcacaa accaggcaac gttgcctttg ccataaggcg cgataatcac
     6961 cgccgccagc aaggggccca gggaactgcc aaagttgccg ccgacctgaa agatagattg
     7021 cgccaggcca tgacgcccgc cggaagccat acgggccacg cgagaagatt ccggatgaaa
     7081 gaccgatgaa ccggtaccga ccagcgccgc tgccagcaga actgcgccga aactgcccgc
     7141 cagcgccagc agcaccagac cacttaaggt aaagcacatg ccaattggca acgaccacgg
     7201 catcggatat ttatcggtcc agtagccgac aactggttgc agtagcgaag aggcgagctg
     7261 gaaggtgagg gttatcatgc caatctgcat aaatgtcaga gaaaactctg actgaagcag
     7321 cggataaatc gccagaatca gcgattggat catgtcgttc agcagatgtg agaggctgat
     7381 agcacctaaa ataccaaacg atgttcgggc cttggtcgtt gacgcagccg cgcccgccac
     7441 aggctgggtt tgttcactca ttgccatagg aaagtcactt tttcagggtt gcgatgtaaa
     7501 gaatgatctt atttgtgatt attacccgac taacatacct gtatgcgccg cctgaaggaa
     7561 gtctcaacgc tgaatacaga atttctaatc tggatacaga tttatcttca ccggacgcag
     7621 acttgtctat gatgtcgcgt catactattt ttcaacacgt tgaaatcagg tcagggagag
     7681 aagtatgaaa ttattgcagc ggggcgtggc gttagcactg ttaaccacat ttacactggc
     7741 gagtgaagct gctctggcgt atgagcagga taaaacctac aaaattacag ttctgcatac
     7801 caatgatcat catgggcatt tttggcgcaa tgaatatggc gaatatggtc tggcggcgca
     7861 aaaaacgctg gtggatggta tccgcaaaga ggttgcggct gaaggcggta gcgtgctgct
     7921 actttccggt ggcgacatta acactggcgt gcccgagtcc gacttacagg atgccgaacc
     7981 tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg gcgatcggta atcatgaatt
     8041 tgataacccg ctcaccgtat tacgccagca ggaaaagtgg gctaagttcc cgttgctttc
     8101 cgccaatatc taccagaaaa gtaccggcga gcgcctgttt aaaccatggg cgctgtttaa
     8161 gcgtcaggat ctgaaaattg ccgttattgg cctgacgacg gatgacacag ccaaaattgg
     8221 taatccggaa tatttcactg atatcgaatt tcgtaagccc gctgatgaag cgaagctggt
     8281 gattcaggag ctgcaacaga cagaaaagcc agacattatt atcgcggcga cccatatggg
     8341 gcattacgac aatggtgagc acggctctaa cgcaccgggc gatgtggaga tggcacgcgc
     8401 gctgcctgcc ggatcgctgg cgatgatcgt cggtggccac tcgcaagctc cggtctgcat
     8461 ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt acgccatgca aaccggatca
     8521 acaaaacggc atctggattg tgcaggcgca tgagtggggc aaatacgtgg gacgggctga
     8581 ttttgagttt cgtaatggcg aaatgaaaat ggttaactac cagctgattc cggtgaacct
     8641 gaagaagaaa gtgacctggg aagacgggaa aagcgagcgc gtgctgtaca cccctgaaat
     8701 cgctgaaaac cagcaaatga tctcgctgtt atcaccgttc cagaacaaag gcaaagcgca
     8761 gctggaagtg aaaataggcg aaaccaatgg tcgtctggaa ggcgatcgtg acaaagtacg
     8821 ttttgtacag accaatatgg ggcggttgat tatggcagca caaatggatc gcactggtgc
     8881 cgactttgcg gtgatgagcg gaggcggaat tcgtgattct atcgaagcag gtgatatcag
     8941 ctataaaaac gtactgaaag tgcagccatt cggcaatgtg gtggtgtatg ccgacatgac
     9001 cggtaaagag gtgatcgatt atttgaccgc cgtcgcgcag atgaagccag attcaggtgc
     9061 ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac ggtaaactga acaacctgaa
     9121 aatcaaaggc gaaccggtcg atccggcgaa aacttaccgc atggcgacat taaacttcaa
     9181 tgccaccggc ggtgatggct atccgcgcct tgataacaaa gcgggctatg tgaataccgg
     9241 ctttattgat gccgaagtgc tgaaagcgta tatccagaaa agctcgccgc tggatgtgag
     9301 tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc gaaagtgccg gatgttcgca
     9361 tccggcacaa tgcttaatcg cgacgggcga tatcagcaaa tttggcatcg aggatctttg
     9421 ccagatcgcc tgccgccagt tcgatatcca gcccgcgctt gccgccggaa acataaatag
     9481 tggcaaattc ttgtgcgggg gcgtcgataa tcgttggcag acgttttttc tgccccagtg
     9541 ggctaatccc ccctaccaga taccccgtcg aacgctgcgc gaccatcgga tcggccatct
     9601 caactttctt ggcacccagc gcttttgcta cttttttaag atccagttga cctgcgaccg
     9661 gcgtaacggc cacggcaagg tgtttcatat caccgttcac tgcgaccagc agcgttttgt
     9721 agacctgatc cggattcaaa cctaattttt tgacgacttc atcgccaaaa ttggtttcag
     9781 ccggatcgtg ctcgtaggtg tggatttgaa acgaaatctt gtttttttcg agtaatttaa
     9841 ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa acgaagcaag catacgcctg
     9901 atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat tggcaggatg gtgagattga
     9961 gcgacaatcg agttacaccg tcgcttaaag tgacggcata ataataaaaa aatgaaattc
    10021 ctctttgacg ggccaatagc gatattggcc atttttttag cgcaacatct gcggcaaatt
    10081 cccttctcca tacaggtgta gtgcaccaac cgcgaccaca tagcgccccg gcggcatggc
    10141 gcataatttg tcccgccagg cgagatttcg ctgatgcatc agcacatcgt acagcgactg
    10201 actgaacgta ttaggcagcg ttatttcatt attttgcggc ggcgcattca gccaccagct
    10261 catcatttgt tgcagcaacc gtgcgttggt gtgccagtgg gtcagcgtat cgtccagcag
    10321 cgccagtccg ttgtcaggga gctggagcaa catggcaatc tggttttcag ccccttccag
    10381 ttcaatcacg ggtttatgtt gttgcttagc cgcctgcaat agctggtaat cgataccgta
    10441 ttctgcccgc agccccagtt tttgcgcctg cgtcgcctga agaaccatcg cgatttgcca
    10501 cagcggttga gtagaaaaga gcgacggaga aatgcccatc tcctggctaa tatgctgcag
    10561 gttttggagt tgctcctcgc taatgcgctt ttccagggcc tcgcaggcag gcaaattggc
    10621 aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc agcgcatcgg cgtttttgag
    10681 ctttttgagc aaacgggtgg gcaggggagc catatcgtgg ctacccatat gaatactgcc
    10741 gatcagatga aaatggcgat tgccgggaag agagatatcg atagcaggcc aggtgtaatg
    10801 attaccgcgc agcgcggccc aaagcgtttt tacccggtac aacagatcca tacgacctcc
    10861 ctttgtgaaa tatcatgcta gcgcgcgggg agggatggcg caaatggagt tgattgttgc
    10921 acttgcgcac agtgcttgtg tgcattacaa tgattaaata atggacaagg agatgagaga
    10981 ggaatggctc aaaagaaaaa tataaggagt tttcgtgatg cctggttggc tgattttttt
    11041 gtccattcaa cgccacatcg aaagatccct gctgaaatcc ataccacgtt atcacgaaag
    11101 ttggacatca ttaatgctgc cacttctcat cgggatttac gctcccctcc ggggaaccga
    11161 tacgaagagt tatccgggaa attacaggaa tactcctcca ttagagtgaa taaacagtac
    11221 cgactgattt ttaaatgggt taatggcaag gccgaagaat tgtttttaga cccacacaac
    11281 tactaaaaca tagctgtcgt attgacgaga aaggtaaaac tatgaaacag gcaacaagaa
    11341 aaccgacgac acccggagat attcttctct atgaatatct ggaaccgctc gatttgaaaa
    11401 tcaatgagtt agcagagttg ctgcatgttc atcgtaatag cgtcagtgca ctgatcaata
    11461 acaatcgtaa actcaccact gagatggcat tccgtctggc gaaagttttt gataccacag
    11521 tcgatttttg gctaaacctc caggcggcag ttgatctttg ggaagttgaa aacaacatgc
    11581 gtacccagga agaattggga cggattgaaa cagtggctga atatttggca cgccgtgaag
    11641 agcgtgcaaa aaaggtcgcg tagaataatt tccctataac ggatgcgcca ccctaaagca
    11701 gtgcatccgc aatgatgtac ttattccttc ggtttaaacc gcagcaaccg gttggcgtta
    11761 ctcactacgg taatcgacga gagcgccatt gccgctccgg cgaccaccgg gttaagcagc
    11821 gtaccggtga acggccacaa aataccggcg gcgaccggaa taccaatgct gttgtagata
    11881 aacgcaccga gcaggttctg cttcatattg cgcagcgttg cgcgggaaat cgcgagcgca
    11941 tccgcaacac ccatcaggct atggcgcatc agggtaatcg ccgcggtttc aatggcaaca
    12001 tcactgccgc cacccattgc aatgccgaca tccgcctgag ccagcgctgg cgcgtcgtta
    12061 atgccatcgc ccaccattgc cacctgacgt ccttcacttt gcagacgttt gatcgcttcg
    12121 gctttaccgt ccggcagcac cccggctatc acctcatcaa tccctgcttc tttggcgatc
    12181 gcattggcgg tggttgggtt atccccggtt agcatcacca gacgatatcc cgctttatgc
    12241 aggcgttgca gcgccgccac gctatcacta cgcaatggat cgcgtacagc cagcagggct
    12301 accgctttcc cgtcaaccgc cagcagcaca ggcgttgccc cttgcgatgc ctgagctgaa
    12361 atatctgctt caatcgcttt agtatcaact tgctggtcat ttaacagcgc ctgattgccc
    12421 agcaataacg catgaccttc agcttcaccg ctcacgccca gcccgcgcaa tgtgcggaaa
    12481 ccgttgacct gcggcagctg catatcgctt gctttatcga ggatcgcccg tgccagcgga
    12541 tggctggaac cttgttccag tgccgccgcc agacgcagcg cctgcgcttc gtcaaaatca
    12601 gcaaatgttt ttactgcgac aacctgcggc ttcccttcgg tcagcgtccc ggttttatcg
    12661 aacaccacgg tgtcgagcgt actggcgcgt tgcagcgcgt cggcgtcccg caccagcacg
    12721 ccaaactcag ccgcccgccc gacgcccgaa ataatcgaca tcggcgtcgc cagtcccagc
    12781 gcacacggac aggcgataat cagcaccgtg gtggcaatca ccagggtata aacaatttgc
    12841 ggtgccgggc caaagaaata ccagattgcc gcactgacca gcgcaatgac taccaccacc
    12901 ggcacaaaca cagcggagat tttatccgcc agttgaccga tttctggctt gctgctctgg
    12961 gcctgtcgca ccatgcgaat gatccgtgac agcgtagtat ggctgccgac cgcactggcg
    13021 cgaaacagca cactgccgtc ctgtaccact gtcccggcat ggacgctatc gccttcgcct
    13081 ttttgctgcg ggattggttc gcccgtcagc atcgcttcat ccagccacgc ttcgccctgg
    13141 gtaatttcac catctaccgg cacccgatca cccgtcgtca ggcgcagcaa catacctggc
    13201 tgaacatctg ccagcggcac gtttttttca ccttcgttgg tgaccaggcg cgccgtcggt
    13261 ggggttaaat cgagtaattt ctccagcgcc ttagaagaac gctgacgtgc gcgcgcttcc
    13321 agcatatgac cgagattaat cagaccgata atcatcgcac tggcttcata gtaaagatgt
    13381 cgcgcttcca tcgggaacca ctgcggccac aggttgacgc tcattgaata gagccacgcc
    13441 acgcccgtac ccagcgccac cagcgtatcc atcgtcgctg caccgttcag caggcttttc
    13501 catgcgctgc ggtaaaaatg gccgccggcg aaaaccatta ccgccagggt tatcaggccg
    13561 ataaccaacc acaggctgcg gttgtcggcg gtgaccatca tgttatcgcc gatcatcccc
    13621 cagaccatca ccgggatacc caccgccagt gcgacaatcg cctgccagcg gaagcgcttc
    13681 atcgtagcga cggcggtttc ttgctggcgc tcgcggcgtt tagcgtcatc ttcaatcgct
    13741 tccgcgccgt agcccgcttt ttccaccgcc tgcactaaat cttgtgggga ggcactgccc
    13801 atcaccagcg cagtacgctc cgccaggttt acccgtgcct gagtgacgcc cggtacgctt
    13861 tgcaacgcat tttgtacgcg ggtgacacag ctggcgcagc tcatgccgct cagcagcaac
    13921 tgctggctgt catcgtcatc ggcggtcgct gccggaagcg cctcagaaac cgctgtcagt
    13981 gcttccgacg ggattgatga ctccgccagc ggtttagcct ttgggtggct tacagatgcg
    14041 tcataacccg cttgtttgat ggtttcgatc agctgttctg cactggcagt cccggtaacg
    14101 tgcgcttcag tgacagacac atccgcctgc tcaacatccg ggcgctgttc gagactttct
    14161 ttcacgcgtt taacgcagtg accgcaggac aggccgtcca gggtcaggtc gatagtttgt
    14221 gacataaaac actcctttta agacagtttt gactggctgt gataaaggtt aaaccttcca
    14281 gcaaggggaa ggtcaagaaa ttaataaacc aggcgggtaa aagtacgtaa agattaaaaa
    14341 atcggctcga tttgcatcag gattagacat ttatctcttt gttttcctgt agttaagttg
    14401 cgggtgctaa gttaaatcag gatgcctgaa aattggcacc ggggtgagga attacctccc
    14461 gcatctataa aaaggagtta acaaaagatg ttagatgcaa acaaattaca gcaggcagtg
    14521 gatcaggctt acacccaatt tcactcactt aacggcggac aaaatgccga ctacattccc
    14581 tttctggcga atgtacctgg tcaactggca gcagtggcta ttgtgacctg cgatggcaac
    14641 atctatcgcg cgggtgatag tgattaccgc tttgcgctgg aatccatctc gaaagtctgt
    14701 acgttagccc ttgcgttaga agatgtcggc ccgcaggcgg tacaggacaa aattggcgct
    14761 gacccgaccg gattgccctt taactcagtt atcgccttag agttgcatgg cggcaaaccg
    14821 ctgtcgccac tggtaaatgc tggcgctatt gccaccacca gcctgattaa cgctgaaaat
    14881 actgaacaac gctggcagcg aattttacat atccaacaac aactggctgg tgagcaggta
    14941 gcgctctctg acgaagtcaa ccagtcggaa caaacaacca acttccataa ccgggccatt
    15001 gcctggctgc tgtactccgc cggatatctc tattgtgatg caatggaagc ctgtgacgtg
    15061 tacacccgtc agtgctccac gctcatcaat actgttgaac tggcaacgct tggcgcgacg
    15121 ctggcggcgg gtggtgtgaa tccgctgacg cataaacgcg ttcttcaggc cgacaacgtg
    15181 ccgtacattc tggctgaaat gatgatggaa gggctgtatg gtcgctccgg tgactgggcg
    15241 tatcgcgttg gtttaccggg caaaagcggt gtaggtggcg gtattctggc ggtcgtccct
    15301 ggcgtgatgg gaattgccgc gttctcacca ccgctggacg aagaaggcaa cagtgttcgc
    15361 ggtcaaaaaa tggtggcatc ggtcgctaag caactcggct ataacgtgtt taagggctga
    15421 tcatgatgaa cacggaaggt aataacgata acaaacccct cggtctatgg aacgtc
//
LOCUS       DAZKGF010000118        15214 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZKGF010000118 DAZKGF010000000
VERSION     DAZKGF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.118"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(277..352)
                     /locus_tag="ACHOQV_003475"
     tRNA            complement(277..352)
                     /locus_tag="ACHOQV_003475"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(317..319),aa:Phe,seq:gaa)
     gene            complement(458..1165)
                     /gene="yqgA"
                     /locus_tag="ACHOQV_003476"
     CDS             complement(458..1165)
                     /gene="yqgA"
                     /locus_tag="ACHOQV_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP9631789.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1318..1395
                     /locus_tag="ACHOQV_003477"
     CDS             1318..1395
                     /locus_tag="ACHOQV_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631790.1"
                     /translation="MHATSLHSLAYRRFARNSSLFLLMR"
     gene            1558..3693
                     /gene="speC"
                     /locus_tag="ACHOQV_003478"
     CDS             1558..3693
                     /gene="speC"
                     /locus_tag="ACHOQV_003478"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFP9631791.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVALGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAAEQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLFGYVLK"
     gene            complement(3743..4999)
                     /gene="nupG"
                     /locus_tag="ACHOQV_003479"
     CDS             complement(3743..4999)
                     /gene="nupG"
                     /locus_tag="ACHOQV_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFP9631792.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5201..6280)
                     /gene="mltC"
                     /locus_tag="ACHOQV_003480"
     CDS             complement(5201..6280)
                     /gene="mltC"
                     /locus_tag="ACHOQV_003480"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFP9631793.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6345..6620)
                     /gene="yggX"
                     /locus_tag="ACHOQV_003481"
     CDS             complement(6345..6620)
                     /gene="yggX"
                     /locus_tag="ACHOQV_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFP9631794.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6648..7700)
                     /gene="mutY"
                     /locus_tag="ACHOQV_003482"
     CDS             complement(6648..7700)
                     /gene="mutY"
                     /locus_tag="ACHOQV_003482"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP9631795.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7861..8580
                     /gene="trmB"
                     /locus_tag="ACHOQV_003483"
     CDS             7861..8580
                     /gene="trmB"
                     /locus_tag="ACHOQV_003483"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP9631796.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8580..8906
                     /gene="yggL"
                     /locus_tag="ACHOQV_003484"
     CDS             8580..8906
                     /gene="yggL"
                     /locus_tag="ACHOQV_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFP9631797.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            9090..9809
                     /gene="yggN"
                     /locus_tag="ACHOQV_003485"
     CDS             9090..9809
                     /gene="yggN"
                     /locus_tag="ACHOQV_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFP9631798.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9985..11031
                     /gene="ansB"
                     /locus_tag="ACHOQV_003486"
     CDS             9985..11031
                     /gene="ansB"
                     /locus_tag="ACHOQV_003486"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFP9631799.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDISKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            11148..12155
                     /gene="yggM"
                     /locus_tag="ACHOQV_003487"
     CDS             11148..12155
                     /gene="yggM"
                     /locus_tag="ACHOQV_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFP9631800.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPIKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSADCYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQTNERWKKDTEQLRRNGVI
                     K"
     gene            complement(12211..13512)
                     /locus_tag="ACHOQV_003488"
     CDS             complement(12211..13512)
                     /locus_tag="ACHOQV_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP9631801.1"
                     /translation="MDSYIALTLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLVILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLIALMMITYIPQISLFIPQLLGLM"
     gene            complement(13512..14015)
                     /locus_tag="ACHOQV_003489"
     CDS             complement(13512..14015)
                     /locus_tag="ACHOQV_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP9631802.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QPAQEVS"
     gene            complement(14060..15046)
                     /locus_tag="ACHOQV_003490"
                     /pseudo
     CDS             complement(14060..15046)
                     /locus_tag="ACHOQV_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT         4030 a         3645 c         3857 g         3682 t
ORIGIN      
        1 cagacactgg aagatgccct gagtttgtat acagatgagc gcacagtgag caagatttgc
       61 aaagggggga ataaatcttg acaggctgaa tcagcaatgt aagcatacag tgttaacgag
      121 aacagggcgt ctctgcaaaa gacaccctgt ttgaggatac gataccgaac tcaatcagtg
      181 taaccacaac ttacacaaac ggagaccctt tgatgccgca tcctgtatta accgcccgtc
      241 aaggatctca tcccttaacc agcgtgttct ggtatatggt gcccggaggc ggaatcgaac
      301 caccgacacg gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc
      361 gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc
      421 acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg
      481 cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt
      541 taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt
      601 ctgccatcat cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta
      661 actggatgat cagtaatggg atactaatta ccgacaccgc aatacccaac gagcaggcga
      721 agatcatcgc cgtaaagaaa tcgagaaatg acttggcgat taaaatactc ggatcgccgg
      781 tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa
      841 tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag tggcgaaaca
      901 gattttgtgc tttggtgacc gcggtattga cgcctttttc cagcagacaa atttcgccga
      961 ttaatgcacc gagtaaggtt gctaaaacca tcgccggaag gttggcgcat ttcaccacca
     1021 gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac
     1081 gttccggtaa acgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag
     1141 cgttgataaa agggccgatg accacaagag ttcctgttaa ctgtattgtc tgattttctt
     1201 attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgct
     1261 ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg
     1321 cacgccacct ccttacattc tctcgcttat cgccgtttcg cgcgaaactc ctcccttttt
     1381 ctgctcatgc ggtgaagtta acgcacgctc actgcaggac aacagtaaaa tcagagcgtt
     1441 tctgctttta ctgatgtctg gcggtcggag ctggtgacca gtttgaccca catctcatgg
     1501 ggcaggtttg tccatcctgt ccggtactct tacttccccg aaacgggttt tgcgcttatg
     1561 aaatcaatga atattgccgc cagtagtgaa ctggtatccc gactttctac tcatcgccgc
     1621 gtggtggcgt tgggagatac tgattttacg gacgtcgcgg cagtcgtcat taccgctgcg
     1681 gatagtcgca gtggcattct tgcgttgctt aagcgcaccg gttttcatct accggtgttt
     1741 ttgtattccg aacatgttgt tgaattacct gcgggcgtta cggcggtaat caacggcaac
     1801 gagcagcagt ggctggagct ggaatccgca gcctgtcagt atgaagagaa tttgctgcca
     1861 ccgttttatg acacgctgac gcagtacgtt gagatgggca acagcacctt tgcttgccct
     1921 ggacatcaac atggtgcgtt ttttaaaaag catcctgccg gacgccattt ttacgatttc
     1981 tttggtgaga acgtctttcg cgccgatatg tgtaacgctg acgtaaaatt gggcgatctg
     2041 cttattcatg aaggttcggc gaaggatgca cagaaattcg cagccaaagt ctttcatgcc
     2101 gataaaacct attttgtgct gaacggcaca tcggcagcga ataaagtggt gacgaatgcg
     2161 ctgttaacgc gtggcgatct ggtgctcttc gaccgtaaca accataagtc gaatcatcat
     2221 ggcgcgttga ttcaggcggg ggcgacgccg gtctatctgg aagcttcacg caacccgttt
     2281 ggtttcattg gtggtattga tgcgcactgt tttaatgaag agtatctgcg ccagcaaatt
     2341 cgcgacgttg cgccagaaaa agcagacctg ccgcgtccgt ttcgtctggc gattattcag
     2401 ctgggaacct atgacggcac tgtctataac gcccgtcagg tgatcgatac cgttgggcat
     2461 ctgtgtgatt acattctgtt tgattccgcg tgggtcggtt acgaacagtt tatcccgatg
     2521 atggcggata gctcgccgct gctgttagaa cttaacgaaa acgatccggg gatctttgtg
     2581 acgcaatcgg tgcataaaca gcaggcggga ttctcacaga cgtcgcagat ccataaaaaa
     2641 gataaccata ttcgcgggca ggcgcgtttt tgcccgcata agcggttaaa taatgccttt
     2701 atgctccatg cttctaccag cccgttctat ccgctgtttg ccgcgctgga cgttaacgcc
     2761 aaaattcatg aaggggagag tgggcgtcgg ctgtgggcgg agtgcgttgc gttggggatt
     2821 gaagcccgga aggcgattct tgcgcgctgt aagctgttcc gcccgtttat tccacccgtt
     2881 gttgatggca aattgtggca ggattatccg acgtcagtgt tagccagcga ccgtcgtttt
     2941 ttcagttttg agccgggggc gaagtggcac ggctttgaag gatatgccgc ggagcagtat
     3001 tttgtcgatc cgtgcaagct gttactgacc acaccaggta ttgatgctga aaccggtgaa
     3061 tatagcgact ttggcgttcc ggcgacgatt ctggcgcatt atctgcgtga gaacggtatt
     3121 gtgccggaga agtgcgatct caactccatt ctgttcttat taacgccggc ggaaagccac
     3181 gagaagctgg cacaactggt ggcgatgctg gcgcagtttg aacagcatat tgaggatgac
     3241 tcgccgctgg cggaggtgtt gccgagcgtt tataacaaat atccggtgcg ctatcgcgac
     3301 tacactttgc gccagttgtg tcaggagatg cacgatctgt atgtcagttt cgacgtcaaa
     3361 gacctacaaa aagcgatgtt ccgccagcag agtttcccgt cagtggtgat gaacccccag
     3421 gatgcgcata gcgcttatat tcgcggtgaa gtggagttgg tgcggattcg tgatgccgaa
     3481 gggcgaattg cggcagaagg ggcgttgcct tatccaccag gcgtgctttg cgtggtaccc
     3541 ggagaagtct ggggtggggc ggttcagcgt tatttccttg cgctggaaga aggggtgaat
     3601 ttattgccgg gattttcgcc ggagctgcaa ggtgtttata gcgaaaccga tgcggatggc
     3661 atgaaacggt tgttcggtta tgtgttgaag taagaataaa aaaaacgggt caccttctgg
     3721 cgacccgttt ttctttgcgt aattagtggc taaccgtctg tgtgcctgtc gggacacgaa
     3781 cgtgtttata tttgaacatc gccatgaacg cgaaggccag aaccacggag taaccagcga
     3841 aaatcagcca tacggtctgc cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt
     3901 taccgctcac gatgccgccg aggatacagc cgaagccgtt agtcatcatc aggaacatcc
     3961 cctgcgcact ggcgcgaatt gccgggctaa cttctttttc gacaaacacc gaaccagaga
     4021 tgttgaagaa gtcgaatgcg caaccgtaaa cgatcatcga cagtaccagc agtacagtac
     4081 cgaacggagt cgggtcgccg taagcaaaca gcgcaaaacg caggatccac gccacaatac
     4141 tgatcatcat tacgttctta ataccgtagc ggcttaagaa gaacgggatg gtcagaatga
     4201 acagggtttc agagatctgc gaaatcgaca tgatgattga cgcatgctgc acgatgaagc
     4261 tgctggcaaa catcggatct ttgtcgaagc tgtgcaggaa ggtgttaccg aacatgttgg
     4321 taatctgcag ttccgcgccc agcagcattg agaagatgaa gaagattgcc atacgcttgt
     4381 ttttaaacag cgcgaatgca tcgaggccca gcagggttgt ccagctctga ttcgcttgct
     4441 gtttagcaac cggaatatgc ggcagagtca gggtaaacag aaccagaatg gcggaaagtg
     4501 ctgcgccaat atacagctgc atgtggctta attcgaagcc agacaggctc accacccaca
     4561 ttgccatgat aaagccgatg gtgccccaga tacggattgg cgggaagtca gtaacgatat
     4621 ccatcccggc attttgcagg cgatagtaag agatggtgtt gattaaccca agcgttggca
     4681 tataagcaaa cgagttaatc aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg
     4741 ccgccatgaa cagcgtgata gcgccaatgg tgtggcaaat ggcatatacc catttcgcac
     4801 ttaaccattt gtcggccaca atccccagca gcgcaggcat aaataccgcg gcgataccca
     4861 gtgagctata aactgcgcca atagaagcac cgtcaaactt cagggtaaca aacatatagg
     4921 agccgagggt cgtcagccaa cttccccaca gacagaactg cagaaaagag aggattttca
     4981 gctgcagctt aagattcatg ttaatttcct cacatcgtga tgcggatgag tgtttgaaaa
     5041 agcacatttg accgtgcgag atgatgggat tctatcaact tcccggactg gttttttgtt
     5101 acctgtggca aataattgca aacattccca atttgcaaaa cgaaattgtg atgtggataa
     5161 catttttacc cctgagcatc gtcaggggcg gttagtggaa ttatcggcgg cggtaggatt
     5221 tttgcgcggt attcacttta taaagataac ggcgagattc cgcagaggga tggcgggtcg
     5281 tcagcgtttg ataaacatcg cccggcgtca tggtgttaat aatattggcc gcctgaatct
     5341 tatcattcga aaagactcgc agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg
     5401 cataacgccg ggatgtaggg ttatcaattc cgccgagata aacgttgttc agcatcgcca
     5461 gatacgcggt gccggtatca atattgctgg caggatcaaa caagaaactg cggctcggcg
     5521 tgccggattt cccctgcgaa cggaacacat ctttcccggc agtatgttgt accacctgca
     5581 ttaaccccag cgcatcggaa cggctgaccg catacgggtt aaaggatgac tcggtctgca
     5641 taatcgccag aatcagcgac tcatcaacgc catatttccg tgacgcctgg cggaccatgc
     5701 cgagatattt gtgcgcacgt ttatcaaggt ggttcggcac catgttaatg gtgacgctgt
     5761 agatgatacg cagcccgttg ctgcggctct tcagacggtt tttcagcaga taatccgcga
     5821 agttgctcgc gcgaccttcc cagcgaatcg gctgcccggt gttgtccacc acctgaccgt
     5881 aaaggaaagg ttctttcgaa atcgtaatat catcaacgtc ggaatagaga tcgaccgaac
     5941 tcggatcgtc ccccatcagc aacgttttga taattgcccg gcgcagatgc gcggcaggtt
     6001 ctgtcccggc gatggtttcg atagtaatcg taccgtcatc gaagttgatg tggctgcggg
     6061 tctgatattg atcggtgtat ttcacgtagt ccttaggacc ggcgatcacc acctctttga
     6121 agccccagat gttctcaata ttgtgggcaa attgccccat cagaatatca aaaccgttgg
     6181 tatctttgac ccaggcttcg ttataggtat cgcctttttt ggtcgtcgaa caggagatga
     6241 gcaacggcgc aatcaaagcc agcgcgagat attttttcat cattccgggt gcgtgttgtg
     6301 tttgttttta tgcaagttgc cggaggcgtg ctccggcact gtttttattt tttatcttcc
     6361 ggcgtatagc cctcaatatg cacctcttta ccctcgaaca ggaagttgac catctcctgt
     6421 tccagcagct tgcggtgctc ggcattcatc atgttgagtt tcttttcatt aatcagcatg
     6481 gtttgtttgt gctgccactg cgcccaggct tctttggaga tctcgttata gatgcgtttt
     6541 cccagctcgc cggggtacag ctgaaaatcc tgaccttctg cttcacgttg caggaaagta
     6601 caaaaaatcg ttctgctcat aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg
     6661 ccagtgcgta actgctgtaa caaacgctcc acgggagccg ccaggccaac tgacggcggt
     6721 tgcgctaagt tataccagag cgcattgcct tcatccatgc agccggtgaa tgacgacacg
     6781 ggaagccaca taggcacaat atctaagtgg aaatggctga aggtatgccg aaacgcggtc
     6841 agctgcgtca ggttatcggc agcaatctgc cgttgcgcca gccactgccg caaactttct
     6901 tcgtcggcaa actgcgggaa acagtataaa ccgccccaca atccgctcgg cggacgctgc
     6961 gccagcaata cttcatcttc gtgctgtaac aggagaaagt agccggtgcg ctccggcagc
     7021 gtctgtttcg gttttttgcc cggataaagc gaccagctat tgttggtggc ggcaatacat
     7081 ccgttttgta gcggacagag cgagcatttc ggcttcgagc gcgtgcaaat catcgcaccc
     7141 aaatccatca tcgcctgatt aaaccgctcc acgccgaccg cgggcgtcac ctgttcgctc
     7201 aaactccaca gtttattctc gacctctttt ttcccaggcc agccgcttac agcatagcag
     7261 cgcgccagca cccgtttgac gttaccgtcg agaatcggaa agtgcttacc cagagaaagc
     7321 gagagaattg cgcctgcggt ggaacgcccg acgcccggta acgccgcgac ttcttcaaag
     7381 gtttccggga atttaccgcc gtgtaaggtc gccacttgtt gtgccgcctt atgcagattg
     7441 cgcgcgcggg cgtaatagcc aagcccggtc cacaagtgga gaacttcgtc gagcggcgca
     7501 ttggcgagat cggtcaccgt cgggaagcgc gccataaagc gttcaaaata ggggataacg
     7561 gtcgcaacct gagtttgttg caacatcact tctgagagcc atactttgta gggcgtcttg
     7621 tcaatttgcc agggcagcgt tttccgcccg tatttatcgt accagtccag aacctgggct
     7681 gaaaattgcg acgcttgcat ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca
     7741 cagcgacggc agggtgtaaa ccggaacttt ccgcagccac ggccttatca tgcttgcatc
     7801 cggcaactaa ctttggataa tgcccgtttt cagaacactt tcacaagcga ctaaatcttt
     7861 atgaaaaacg acgtcatctc accggaattt gatgaaaacg gccgcccact gcgccgtatc
     7921 cgtagttttg tgcgccgcca ggggcgactg accaaaggcc aggaacatgc gctggaaaac
     7981 tactggccgg tgatgggcgt tgagttcagc gaagatatgc tggatttccc cgcgcttttt
     8041 ggccgagaag cgccggtgac gcttgagatt ggtttcggca tgggggcgtc gctggtggca
     8101 atggctaaag atcgccccga gcaggacttc ctcggcattg aagtgcattc accgggcgtt
     8161 ggtgcgtgcc tggcttctgc gcatgaagag ggcttaagca acctgcgcgt gatgtgtcac
     8221 gatgcggttg aagtgctgca taaaatgatt cctgacaatt cattgcgcat ggtgcagctc
     8281 tttttccctg acccgtggca caaagcgcgc cataataaac gccgtatcgt tcaggtgccg
     8341 tttgccgaac tggtaaaaag caaactgcaa ctggggggcg tattccatat ggcgaccgac
     8401 tgggaacctt atgcggaaca tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac
     8461 ctgtcagaga gcaatgatta cgtaccgcgt ccggcatcac gtccggtgac gaaatttgaa
     8521 caacgtggtc atcgtcttgg tcacggagta tgggacttaa tgttcgagag ggtgaaataa
     8581 tggcaaagaa ccgtagccgt cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat
     8641 taggattttc ggtggcatgg cgattcccgg aaggtacatc ggaagaacag attgataaaa
     8701 ccgttgatga ctttattaac gaggttatcg aaccgaacaa actggccttt gacggcagcg
     8761 gttatctggc ctgggaaggt ctgatctgca tgcaggaaat cggcaaatgc accgaagaac
     8821 atcaggcgat tgtgcgtaag tggctggaag agcgcaaact gggcgaggta cgcaccagcg
     8881 aacttttcga cgtttggtgg gactaagaaa gcatacgggc gatgacaaat gcaaaactgc
     8941 ctgatgtgct acgcttatca ggcctataaa gatgcacgat cgagtaggct ggataaggcg
     9001 tttacgccgc atccggcatg gaaaacgcgc actttgttat caatctgggg ccagcaaatg
     9061 ctggcctgat ttgttcttga gggaagacta tgatgcgcaa aatgctgctg gcggcagcac
     9121 tttcagtgac ggcaatgacc gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg
     9181 atgtgattgt cagcccgcaa accgtgcagg tgaagggcga aaacggcaat ctggtgatca
     9241 cgccagacgg caacgtgatg tataacggta agcaatattc cctgaatgcc gcccagcgcg
     9301 agcaggcgaa ggattatcag gctgaactcc gtagcaccct gccgtggatt gatgaaggcg
     9361 cgaaaagccg cgtcgaaaaa gcccgtattg cgctggataa aattatcgtt caggagatgg
     9421 gcgaaagcag caaaatgcgc agccgtctga ccaaacttga tgcgcagctg aaagagcaga
     9481 tgaaccgcat tatcgaaacg cgcagcgatg gcctgacgtt tcactataaa gccattgatc
     9541 aggttcgcgc cgaaggccag caattagtga atcaggcaat gggtggaatt ttacaggaca
     9601 gcattaatga aatgggcgcg aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg
     9661 tgctgggaag cctgggcggt ctgcaatcct caatccaaac cgagtggaaa aagcaggaaa
     9721 aagatttcca gcagtttggc aaagatgttt gtagccgcgt tgtgactctg gaagatagcc
     9781 gcaaagccct ggtcgggaat ttaaaataat cctctatttt aagacggcat aatacttttt
     9841 tatgccgttt aattcttcgt tttgttacct gcctctaact ttgtagatct ccaaaatata
     9901 ttcacgttgt aaattgttta acgtcaaatt tcccatacaa agctaaggga taatgcgtag
     9961 cgttcacgta actggaggaa tgaaatggag tttttcaaaa agacggcact tgccgcactg
    10021 gttatgggtt ttagtggtgc agcattggca ttacccaata tcaccatttt agcaactggc
    10081 gggaccattg ccggtggtgg tgactccgca accaaatcta actacacagc gggtaaagtt
    10141 ggcgtagaaa atctggttaa tgcggtgccg caactgaagg acattgcgaa cgttaaaggc
    10201 gagcaggtag tgaatattgg ctcccaggac atgaacgatg atgtctggct gacactggcg
    10261 aaaaaaatta acaccgactg cgataaaacc gacggcttcg tcattaccca cggtaccgac
    10321 acgatggaag aaaccgctta cttcctcgac ctgacggtga aatgcgacaa accggtggtg
    10381 atggtcggcg caatgcgccc gtccacgtcc atgagcgcag acggtccatt caacctgtat
    10441 aacgcggtag tgaccgcagc tgataaagcc tccgctaatc gtggcgtgct ggtggtaatg
    10501 aacgacaccg tactggacgg tcgcgatgtc accaaaacca ataccaccga cgtagcgacc
    10561 ttcaagtctg ttaactacgg tcctctggga tacattcaca acggtaagat tgactaccaa
    10621 cgtaccccgg cacgtaagca caccagcgat acgccgttcg atatctctaa gctgaatgag
    10681 ctgccgaaag tcggcatcgt ttataactac gctaacgcat ccgatcttcc ggctaaagca
    10741 ctggtagatg cgggctatga tggcattgtt agcgctggtg tgggtaacgg caacctgtat
    10801 aaaaccgtat ttgacactct ggcgaccgcc gcgaaaaacg gcactgcagt cgtgcgttct
    10861 tcccgcgtac cgacgggcgc taccactcag gatgccgaag tggatgatgc gaaatatggc
    10921 ttcgtggcct ctggcacgct gaacccgcaa aaagcgcgcg ttctgctgca actggctctg
    10981 acgcaaacca aagatccgca gcagatccag cagatcttca atcagtacta atcacctcgc
    11041 cccggtaacg tgccggggct ttttcacttc agacttacgt ccattgccaa ttttcattac
    11101 cctgatgata atcactggaa taaattattc cgcgcgaggg ttttcgggtg aaaaagcaat
    11161 ggattgttgg cacggcgctg cttatgttga tgactggtaa tgtctgggca gatggcgaac
    11221 cgccaactga aaatatctta aaagatcaat tcaaaaagca gtatcacggc attctcaagc
    11281 ttgatgtcat caccttaaaa aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg
    11341 aaggcgatgt ctcttccagt gacgatctct atacctgggt cggtcagttg gcagattacg
    11401 aactgctcga acagacctgg acgaaagata aaccgataaa attctcggcg atgttaacca
    11461 gtaaaggaac gcccgcgtct ggctggtcgg tgaactttta ctcttttcag gcggcagcca
    11521 gcgatcgtgg acgggtggtc gacgatatca aaacgaataa taaatatctg atcgtgaata
    11581 gcgaagattt caattatcgc tttagtcagc ttgaatcagc gttgaataac cagaagaact
    11641 cgattcctgc gctggaaaag gaagtgaaag cgcttgataa gcaaatggtg gcggcgcaga
    11701 aagcagcgga tgcatattgg ggaaaagatg caaacggtaa gcaaatgacc cgcgaagacg
    11761 cctttaaaaa aattcaccaa cagcgtgatg agtttaataa acagaacgat agcgaggcgt
    11821 tcgcggttaa atatgacaaa gaggtttatc aaccggcgat tgcggcatgt cataaacaga
    11881 gtgcagattg ttatgaagtg ccgattcagc agaagcgaga tttcgatatc aacgaacaac
    11941 ggcgacagac ttttctgcaa tcacaaaaac ttagccgtaa attgcaggat gactggataa
    12001 cgctcgaaaa agggcaatat ccgttaacga tgaaagtttc agaaataaac agtaaaaaag
    12061 tcgcgatttt gatgaaaatt gatgatatca accagactaa tgaacgctgg aaaaaagata
    12121 ccgaacaatt gcggcgcaat ggagtgatta agtaactcat gttaagtacc tttgccggat
    12181 aatataaaaa gttatccggc aaattatttt ttacattagc cctagtagct gtggaatgaa
    12241 caaggatatt tgcgggatgt aagtgatcat catcaacgcg attaacaatg cggcataaaa
    12301 tggcagcaat ggtttgatca aatgttgaat cttcacacct gaaatagaac aaccaacaaa
    12361 tagcgcacta cccaccggcg gtgttaacag cccaatacaa aggttagcca ccatcataat
    12421 accgaaatgt accggatcca ttcccagctc ttgtgcaatc ggcaagaaga ttggtgtgaa
    12481 gattagtaca gccggtgtca tgtccataaa gatgccgaca attaataaga cgatattaat
    12541 cagcagcaat atgaccagtg ggttatcaga aatccccatc aacgcgtcgc tgatcatata
    12601 aggaatatca gcattggtca ttgcccagga catgcccacg gaaaaaccga tcaacagtaa
    12661 tacaatcgaa gtggtaacaa ccgattccag aatcagtttg ggtaaatcgc gccattttac
    12721 ttcccggtaa atcagcaccg agagaataaa tgtgtagaca acggcaattg ctgacgcttc
    12781 agttgcggta aaaatgccgc ccagaatgcc gcccattaca atgaacacca gaagcaaact
    12841 tggtaaggca tccagaaacg ctttacatgc ctgagcaaag gttgctcttt ctgaaagtgg
    12901 gtacccacgg cgtttagcaa taatgccgca gactatcatt accgcgagac ccattaagat
    12961 ccctggcaaa tatcccgcca taaatagcga tgctacagaa acgccgccag ctgtcaggga
    13021 aaacacaatt aacacgtttg agggcggtat cagtaatcca gtaatacaag atgagacgtt
    13081 tacagcggta gaaaatgccg gatcataacc ttctttggtc tgaatgggat tcagtgtgcc
    13141 accaacggca gcagctgccg caacggctga accagaaatg gaaccaaaca tcatattcgc
    13201 caggacgttc acatgaccaa gcgaaccagg aacgcggcca accattacct gggcgaggtt
    13261 aatcaaacgt atcgcaatac cgccgctgtt catcagcgtt ccggcaaaga tgaaaaaggg
    13321 gattgccaat agggcaaagt tatccagtcc attagccagt cgctgcgaaa cagtgattgc
    13381 cgcgatatcc cagggaaaca tcaacagcat tgaagctaca gtggcaatac cgattgagaa
    13441 cgaaatgggc acaccaataa agaccagcac aaaaaaacag ccaaacagtg taagcgcgat
    13501 ataactatcc attaactgac ctcctgcgca ggttgtttca gttgttgaat gctttgatta
    13561 ataaaacaca aggcataaaa catcatcacc aatccactta atggcagaat gatatatacg
    13621 tatcccatcg gtatttgcat cgcggcagat acctgtgatg ttgccagtgt tttatcaatt
    13681 aatttcaggc caccagtcac aatgaccgaa ccggcaaaac ctaaaataag aacgttaatg
    13741 actatactaa gaagtaattg cttgcgctta ttcagggcga gagcaaataa atcaatagag
    13801 agatgtctct gcgcgccgac ggtatatgca gcacctaata atcctaccca gatcatcaaa
    13861 aagcgtgcca gctcatcggt taatgtactt ggctggttaa gtacgtagcg gctaaatact
    13921 tgccagacta cgcatacaac cagagcaagc attacggcga cagaaaacgc ggcaatgacg
    13981 cggtctaccg caagtttcag gcggttaaat atcattgtaa gcatcctgga aaatcagggc
    14041 cagagactgg cccgaaatat tattgagcag cgttgtcgaa tttctctaac aaagcagctt
    14101 gttttggatc ttttttgaag tcgtcaaaca gaggctgaac tgcggcacgg aatggggctt
    14161 tatcaacctt aacgatttca ccacccatgg ctttcgcctg agcacgtgtg tcagcgtcaa
    14221 ttttttccca caatttttgt tgataagctt cggatttctt cgccgctgtt tcgaggattt
    14281 gttgctgttc tggggttaat ttattccatg ttttggtgga gataaccaaa aagtcaggaa
    14341 ttgaggcatg ttcatcttca gaaaagactt tggcaatttc gatatgtcga gtctgcaccc
    14401 aggaaggcac gttattttct gcgccatcta ctacaccttg ctgcatcgca gtataaactt
    14461 cgccaaatga aattggagtg ggtgagccac ccatcagttc aatcattttg atggtggtcg
    14521 ggcttggctg aacacgaatt ttcagccctt taagatcttc tggcttagta atcggttttt
    14581 tcgcatagaa actgcgtgta cccgctacat aagctgataa agcaaaaaag cctttatctt
    14641 ttgttgaatc cataatttct ttaccaactt caccaaaaac cactttgttg aagtgcgcct
    14701 gatctttaaa taagaacggc aaattataga tagcataaat attatcaaaa gactccagat
    14761 cactggcgga acctttggtc atatccagtg caccattttg caacaactac atagtttctc
    14821 gcgcattacc catctggctg ctggggtaaa tacggatcac catattacct tttgacagct
    14881 gttttacttc tttcgccagc tcttcaaaac tctgatgtac aacatgactt ctttccaggt
    14941 tgtgtgctaa ctttaacgtc actttttctg cggcgtttac gccagtgcta accagagcca
    15001 gcaaggatgc gcagactaat gagcgggtaa atgacctcat tttcataaaa aaactccctg
    15061 ttgaagaatg gaaattggtt gatatctaat tgtgcttatc agtgctttgt gcgacaaatt
    15121 ggttaacctg tctgacaact tgtctatgtt tatcacaact tgaaataaag tcagaaatta
    15181 aatattaatt ttgtgcgcca ggttgtacat gttt
//
LOCUS       DAZKGF010000119        15179 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZKGF010000119 DAZKGF010000000
VERSION     DAZKGF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.119"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            25..101
                     /locus_tag="ACHOQV_003491"
     tRNA            25..101
                     /locus_tag="ACHOQV_003491"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Arg,seq:acg)
     gene            382..948
                     /gene="yqaB"
                     /locus_tag="ACHOQV_003492"
     CDS             382..948
                     /gene="yqaB"
                     /locus_tag="ACHOQV_003492"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFP9631803.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            945..1373
                     /locus_tag="ACHOQV_003493"
     CDS             945..1373
                     /locus_tag="ACHOQV_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFP9631804.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1446..3002
                     /gene="gshA"
                     /locus_tag="ACHOQV_003494"
     CDS             1446..3002
                     /gene="gshA"
                     /locus_tag="ACHOQV_003494"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFP9631805.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFIAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            3152..3667
                     /gene="luxS"
                     /locus_tag="ACHOQV_003495"
     CDS             3152..3667
                     /gene="luxS"
                     /locus_tag="ACHOQV_003495"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP9631806.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3718..4830)
                     /locus_tag="ACHOQV_003496"
     CDS             complement(3718..4830)
                     /locus_tag="ACHOQV_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9631807.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALVTQPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYISEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFVWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWKQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(4827..5534)
                     /locus_tag="ACHOQV_003497"
     CDS             complement(4827..5534)
                     /locus_tag="ACHOQV_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HFP9631808.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINADYWQDLQAITQLVHTEK
                     LDGENNCLNRYGVFARSHAAPTQSAWTYKIRQRWQLLKNDLGDLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGAFDPWDTQTGQPCTLEGIVSRNRDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFDYGEQS"
     gene            complement(5792..7330)
                     /gene="emrB"
                     /locus_tag="ACHOQV_003498"
     CDS             complement(5792..7330)
                     /gene="emrB"
                     /locus_tag="ACHOQV_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFP9631809.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(7347..8519)
                     /gene="emrA"
                     /locus_tag="ACHOQV_003499"
     CDS             complement(7347..8519)
                     /gene="emrA"
                     /locus_tag="ACHOQV_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFP9631810.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(8646..9176)
                     /gene="emrR"
                     /locus_tag="ACHOQV_003500"
     CDS             complement(8646..9176)
                     /gene="emrR"
                     /locus_tag="ACHOQV_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFP9631811.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(9267..9602)
                     /gene="ygaH"
                     /locus_tag="ACHOQV_003501"
     CDS             complement(9267..9602)
                     /gene="ygaH"
                     /locus_tag="ACHOQV_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFP9631812.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(9592..10329)
                     /gene="ygaZ"
                     /locus_tag="ACHOQV_003502"
     CDS             complement(9592..10329)
                     /gene="ygaZ"
                     /locus_tag="ACHOQV_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFP9631813.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(10453..11637)
                     /locus_tag="ACHOQV_003503"
     CDS             complement(10453..11637)
                     /locus_tag="ACHOQV_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9631814.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRTL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLPWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASVWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AI"
     gene            complement(11829..12821)
                     /gene="proX"
                     /locus_tag="ACHOQV_003504"
     CDS             complement(11829..12821)
                     /gene="proX"
                     /locus_tag="ACHOQV_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFP9631815.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(12879..13943)
                     /gene="proW"
                     /locus_tag="ACHOQV_003505"
     CDS             complement(12879..13943)
                     /gene="proW"
                     /locus_tag="ACHOQV_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFP9631816.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(13936..15138)
                     /gene="proV"
                     /locus_tag="ACHOQV_003506"
     CDS             complement(13936..15138)
                     /gene="proV"
                     /locus_tag="ACHOQV_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFP9631817.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
BASE COUNT         3735 a         3922 c         4029 g         3493 t
ORIGIN      
        1 agcagtaaaa taaattttcc caatgcatcc gtagctcagc tggatagagt actcggctac
       61 gaaccgagcg gtcggaggtt cgaatcctcc cggatgcacc atattctccg taaccttcag
      121 caatgaaggt atcgaagagg tagcggtatt aaccgcaggc accagggagg ataacgttgc
      181 gccagcagcg gcccgaaggg cgaacgccag tgagtcatcc tcccggatgc accatcttct
      241 ccaaaaaatt tccttcaaaa atatctcatt tctaaataac tactttatcc atttcctgaa
      301 tattcttctc cttctccatc agcgacaaaa tcaagcaatt acgctaaagt agcgtcatat
      361 ttttcgactt gcgagaagac catgtacgag cgttatgcag gtttaatttt tgatatggat
      421 ggcacaatcc tggatacgga gcctacgcac cgtaaagcgt ggcgcgaagt attagggcac
      481 tacggtcttc agtacgatgt tcaggcgatg attgcgctta atggctcgcc cacctggcgt
      541 attgctcagg cgattattga gctgaatcag gccgatctcg acccgcatgc gttagcgcgt
      601 gaaaaaacag aagcagtaag aagtatgctg ctggatagtg tcgagccgct tcctcttgtt
      661 gaagtggtga aaagctggca tggtcgtcgc ccaatggctg taggaacggg gagtgaaagc
      721 gccatcgctg aggcattgct ggcacacctg ggattacgcc gttattttga cgccgtcgtc
      781 gctgccgatc acgtcaaaca ccataaaccc gcgccagaca catttttgtt gtgcgcgcag
      841 cgtatgggcg tgcaaccgac gcagtgtgtg gtcttcgaag atgccgattt cggtattcag
      901 gcggcccgtg cagcaggcat ggacgccgtg gatgttcgct tgctgtgagt gaagcgttat
      961 cgcttttctc gttgtttgcc agtagttttc tcagcgctac actattaccc ggcaactccg
     1021 aagtcgtgct ggtggcaatg ttgctttccg ggatcagtca tccctgggtt ttagttttaa
     1081 cagcaacaat gggtaatagc cttggagggt taactaacgt tatccttggg cgtttctttc
     1141 cattgcgtaa aacatcgcgc tggcaagaga aagctaccgg ctggctgaaa cgctatggtg
     1201 cagtcacgct attattaagc tggatgcccg tggttggcga tttactgtgt ctgttagcgg
     1261 gatggatgcg catctcgtgg ggaccggtaa tctttttttt gtgccttggt aaagcgttac
     1321 gctatgttgc ggttgcagca gctaccgttc agggcatgat gtggtggcac taattgtagg
     1381 cctgcacata tggtcaccat tacagttatg ctaattaaaa cgattttgac aggcgggagg
     1441 tcaatttgat cccggacgta tcacaggcgc tggcctggct ggaaaaacat cctcaggcgt
     1501 taaaggggat acagcgtggg ctggagcgcg aaactttgcg tgttaatgct gatggcacac
     1561 tggcaacaac aggtcatcct gaagcattag gttctgcact gacccataaa tggattacta
     1621 ccgattttgc ggaagcattg ctggaattta ttacaccagt ggatggtgat attgaacata
     1681 tgctgacctt tatgcgcgat ctgcatcgct atacggcgcg caatatgggc gatgagcgga
     1741 tgtggccgtt aagtatgcca tgctacatcg cagaaggtca ggacatcgaa ctggcacagt
     1801 acggcacttc taacaccggt cgctttaaaa cgctgtaccg tgaagggctg aaaaatcgct
     1861 acggcgcgct gatgcaaacc atttccggcg tgcactataa tttctctttg ccaatggcat
     1921 tctggcaagc gaagtgcggt gatatctcgg gcgctgatgc caaagagaaa atttctgcgg
     1981 gctatttccg cgttatccgc aattactatc gtttcggttg ggtcattcct tatctgtttg
     2041 gtgcgtctcc agcgatttgt tcttctttct tgcaaggaaa accaacgtcg ctgccgtttg
     2101 agaaaaccga gtgcggtatg tattacctgc cgtatgcgac ctctcttcgt ttgagcgatc
     2161 tcggctatac caataaatcg caaagcaatc ttggtatcac cttcaatgat ctctacgagt
     2221 acgtggcggg ccttaaacag gcaatcaaaa cgccatcgga agagtacgcg aagattggta
     2281 ttgagaaaga cggtaagcgg ctgcaaatca acagcaatgt gctgcagatt gaaaacgaac
     2341 tgtacgcgcc gattcgtcca aaacgcgtta cccgcagtgg tgagtcgcct tctgatgcgc
     2401 tgttacgtgg cggcattgaa tatattgaag tccgctcgct ggatatcaac ccgttctcgc
     2461 cgattggtgt agatgaacaa caggtgcgat tcctcgacct gtttatggtc tggtgtgcgc
     2521 tggctgacgc accggaaatg agcagtagcg aacttgcctg tacacgcgtt aactggaacc
     2581 gggtgatcct cgaaggtcgc aaaccgggcc tgacgctggg tatcggctgc gaaactgcgc
     2641 agttcccgtt accgcaggtg ggtaaagatc tgttccgcga tctgaaacgc gtcgcgcaaa
     2701 cgctggatag cattaacggc ggcgaagcgt atcagaaagt gtgtgatgaa ctggtcgcct
     2761 gcttcgataa tcccgatctg actttctctg cccgtatctt aaggtctatg attgatactg
     2821 gtattggcgg aacaggcaaa gcgtttgcag aagcctaccg taatctgctg cgtgaagagc
     2881 cgttggaaat tctgcgcgaa gaggatttta tagccgagcg cgaggcgtct gaacgccgtc
     2941 agcaggaaat ggaagccgct gataccgaac cgtttgcggt gtggctggaa aagcacgcct
     3001 gacagaaaag aaaaaggcca ctcgtgagtg gccaaaattt catctctgaa ttcagggatg
     3061 atgataacaa atgcgcgtct ttcatatact cagactcgcc tgggaagaaa gagttcagaa
     3121 aattttaaaa aaaattaccg gaggtggcta aatgccgttg ttagatagct tcacagtcga
     3181 tcatacccgg atggaagcgc ctgcagttcg ggtggcgaaa acaatgaaca ccccgcatgg
     3241 cgacgcaatc accgtgttcg atctgcgctt ctgcgtgccg aacaaagaag tgatgccaga
     3301 aagagggatc cataccctgg agcacctgtt tgctggtttt atgcgtaacc atcttaacgg
     3361 taatggtgta gagattatcg atatctcgcc aatgggctgc cgcaccggtt tttatatgag
     3421 tctgattggt acgccagatg agcagcgtgt tgctgatgcc tggaaagcgg caatggaaga
     3481 cgtgctgaaa gtgcaggatc agaatcagat cccggaactg aacgtctacc agtgtggcac
     3541 ttaccagatg cactcgttgc aggaagcgca ggatattgcg cgtagcattc tggaacgtga
     3601 cgtgcgcatc aacagcaacg aagaactggc gctgccgaaa gagaagttgc aggaactgca
     3661 catttagtca gtttaagcag aagaggccgg gctggtgaaa acaccgcccg gcttttgtta
     3721 cctgtcagtc atccgatatt ccacgctatg tgcttcatca ggttgaggaa cttccagcct
     3781 tgaggccatc cgcatcacga ccgcttcagg aactgcatac tcgcgtctgg cattttgctg
     3841 cttccattgc gcccatggca cttccagata aacgattttt actcgtgcgc cgtaagccgt
     3901 aaacagactg atgagctggc tgcgcagctg gcgggtaatg ttcgttgcat tccagacgaa
     3961 cggctttttc tggcgtaaaa aaacgcgcgc ctcttctttt gcctgttgca caatacgccc
     4021 ggtggcggtt ttgtcgtccg gactggcatt gattcgccgc cgcatatcat caaggctgat
     4081 aacgtccatt ccctggcact gttcactgat atagcgatct ttgcccatac ccggtaagcc
     4141 acacagcaaa ataacctcaa aaggttcagc ctcccaggga acaaaatcag gagaactttg
     4201 ctcatgagtc aggtagtgcc agcgtgcgga atcagaaacg aacgggcgca tttttcccca
     4261 gcattgttgc tcatggcaaa acagctcgaa cagatcgatg cgttccagca ttgattgctg
     4321 atccgggctt tggcgaccaa gcagatcggc acgagcaagc aaagcgagca gacgcgtgtc
     4381 gatgcgcatt gcagccgtga gcagcaaacg ctccggttcc ggacgttcca gtaaccatag
     4441 cggtaaccca tgcagacgca ccagcgcaac aatttgctcg cgtagcacga acggcgttgg
     4501 gatatcgcgc cataaaattt gtcgggccgt cagttcgccc cgacgagcat ggccaggcga
     4561 ctgaatacgt ccgttttcct gcacggtagt gctgcgcttt tcaacatcat gcaataacgc
     4621 cgccgcccat aaaacttcct gctgttgagc ggggagttgc tggaattctg gctgagtaac
     4681 gagggcgttc agcaccattt cggtatgcac gccgacgtca ccttcaccat gatgctccgg
     4741 gtcttgtggc gtgtgatgca tctcctcaac ccagctaaaa cgctgacgta gcgccgacca
     4801 gcgtttgtcg tcggtaagct gccaaatcat gactgttctc cataatcgaa ctcatgggcc
     4861 atccgcgccc gttgccagtg tcgtttccag tggacggtgg ttttgacatg atttttgcgc
     4921 acatacttga agacgttatg cgaaaagtca gcgacggaaa acgcatcacg attcctgctg
     4981 acaatgcctt ccagcgtaca gggttgcccg gtttgcgtat cccagggatc gaacgcgccg
     5041 cgcgcattgg tcagcgccag aaaatcacgt tgccagcttt tttcatcatt ccccggttgc
     5101 gggccggaga tttcgggaac gcaggggaaa tcaaacagag cggcgtaaaa ctgcacctct
     5161 tcccagctca gccacatatc ctggcagcga acggcaaaca gataaaaatc ctgctccagg
     5221 gcgcgatatt caatcgaatg cacggcataa agattttcac caaacaactc cagatcgccg
     5281 agatcgtttt tcagcagttg ccagcgttgg cgaattttat aggtccatgc cgactgcgta
     5341 ggcgcggcat gggaacgggc aaacacgccg tagcggttaa ggcagttatt ttcaccgtcg
     5401 agtttttctg tgtgcaccaa ttgcgtgatg gcctgtaaat cctgccagta atcggcatta
     5461 atgcggtcat cgctggtggt gccgggggag aaaggataat gccaggttct cccgtatttt
     5521 cgttgggtat tcataataat ccattatttt gtgtgtaata aatgtacgcc cgcgagggtg
     5581 cgagttatgg ggttaaaaga aaaatgtgag tatcacccgc gaaagcgagg gcgaaagaaa
     5641 aagagaatta acaacctgag cgaatgctca gaaccggtta atggtgaatg gaagagtttg
     5701 gggcggcgaa aagatacacc gaatgagtgg ataaaacaac cgtttgcaaa atgagcaaat
     5761 aaaaagggag ccgcagctcc ctttgacaac cttagtgcgc accgcctccg ccgccacctg
     5821 caccaaatgg cggtttagca aaccacacca gccccagcag gacgaggaat attccggctg
     5881 acatccagaa gatctcattg gcggaaataa tcagcccctg attagtgatc tgctgggcaa
     5941 tccagcctga tgcctgttgt tgcgtcatcc ccagcccttc cagttggctg tacatcgcct
     6001 gagcgttcgg gttaaacggg tttaccgact cggttaactg tgcgtggtgc atcgactcgc
     6061 ggttagtcca catggtggtg gtgatggatg tgccgataga ccccgccagc gttcgcgtaa
     6121 agttggagag gctcgatgcc gccgccagtc gttccggtgg caaaccggac agcgtaatgg
     6181 tggtcagcgg cataaagaag caggccaccg caaacccctg gataaactgc ggccaggccg
     6241 acgcgccaaa atccatacct ggttcaaagg tataggcgcg ccagtagaag cagacggcat
     6301 acataataaa gctgaaggtt accagccgcc gcatatccag tttatgcgcg aagcggccga
     6361 taatcggcga caggatcacc ggaataatcc ctaccggcgc agaggccaga cctgcccacg
     6421 ttgccgtgta accgtagacc tcctgcaaca actgcggcag cagaacaatc gcgccgaagt
     6481 agagcatata cgcgaggctg atacacaagc agccgatggt gaagttgcgc gacttaaaca
     6541 acgacagatc gactatcggg ttatcgtcgg tcagctccca gacaatcagg aagcagatag
     6601 ccaccactgc caccacggta aggatgataa tttcctgtga tgagaaccag tccagctctt
     6661 taccgcggtc gagcataatc tgcaggctac cgataccaat aaccagcagt gccagcccca
     6721 cggcatcaat ccgccgccgt tcggtgcggg tttcacgtcc gcgcagggtt tgcagtgtca
     6781 tcaacaccac cgccacgcca atcggtacgt tgatgaagaa tatccagccc cagtggtaat
     6841 tgtcactgat atacccgccg aggatcgggc cgcaaattgg cgcgacaatc accgtcatcg
     6901 accacaacgc cagcgcgatc gagcgtttgg ctggcgggta gttattaagc aatagacttt
     6961 gcgaaagcgg gatcagcggc ccggcgacaa tcccctgaat cacgcggaag aagatcagca
     7021 tattcaggct gctggagaca ccacacgccc acgacgcaat agcaaaggcg atggtggacc
     7081 agaggaacag tttcacttcc ccgacgcgct ttgccagcca gccggtaagc gggatcgaga
     7141 tggcattcgc caccccgaaa gaagtgatta cccacgttcc ctggctgagc gatgagccca
     7201 gattcccggc gatagtgggg atcgccacgt tagcaatggt ggagtccagc acctgcatga
     7261 atgtcgccag tgacagcgca atcgtcataa tgaccagttg cgcgccttcc agcggttttt
     7321 gctgttgcat cacacgcacc tctggattag ccagcgttag cttttacgat atcgtcgatc
     7381 agtttattga caggtgccag gctgatttca cgcgcggtgc ttaccgctac cggtgtggaa
     7441 cgtactttat ttgccagtac ctgaccgtca cggttagtgg tattgacgct caccagcgtg
     7501 gacaaaccga tacgcagcgg gtattgctcg agctgtttct ggtccagttc aatacgaaca
     7561 ggcagacgct gaacgacttt gatccagtta ccggttgcat tttgcgccgg aagcagtgag
     7621 aacgcgctac ctgtgcccat atccagacca accacttttc cggtgtattt cacatcatcg
     7681 ccgtaaatat cggtggtgat ggtgaccggc tgaccgatac gcatattggc aatctgcgtc
     7741 tctttaaagt tggcatccac ccacatattg gtggctggaa cgaccgccat cagcggcgtc
     7801 gttgggctaa tttgcgcccc aggctgtacc gcgcggcggg agacataacc ggtcatcgga
     7861 ctgacaatac gggtacgctc cagcgccagc caggcgttac gtacttcggt ggcagcctgt
     7921 tgcacggcag gctgatcttc cagtttagtc cccagaatca tcgcctgatt ggcattgtat
     7981 tgttgaatag cgacgtccag ttgtgcctgg gcactggtaa cggcgtcgcg ggcgtgttgc
     8041 agctcttcgc gaccaatcag gttggcattg cccagcggca cacggcggtt gtagtcgctt
     8101 tgtgctttcg cgagggcgat tttttgcacc tcaatattcg cctgcaactg tttgctgtta
     8161 atcatcagct ggtgggtttg gcgaacgctg gaagccagtg cagttttggc tttttcaaac
     8221 gcctggcgag catctgtcgg gtcgagagtg accagcacat cgccttcttt tacaaaatcg
     8281 gtgttatcgg cccagacttt cgtcacgctg ccagacacct gagacataat ttgaatctga
     8341 ttccctgcca cgtatgcgtc atcagtttct tcgaaatgac gcagtaccaa aaaccaataa
     8401 atccctatcg ctacggcaat aattataaag agcaaggtga gaaggaggag cagacgctta
     8461 cgtttgccgc tcttctttac cggttgctgc ggggtttgag tctccgcatt tgcgctcata
     8521 ttgttctcca cgatcttctt aactcatcgg ctgagccgac ctgcttgcca aaaaggccag
     8581 catgttgcga tgctggccag tcattttttc ttttataaat ctggattttt gagcgagatg
     8641 acgcgttagc tcatcgcttc gagaaccaca ccgtcttgtt ccatctggtc gagacgggag
     8701 agcaatttgc gggtgatttg ctcgagctga tctttttctg ttgtgctgag cgcggaccag
     8761 agttgatgca ggcagttatg ctgcggtggt aaaacctcgc gcaaaaactc gtgacctttt
     8821 tccgttaatt gcagatgcag gcagcggcga tcgttatcgc tttcacgacg ttcgatccaa
     8881 ccgcgttttt ccagttcatc ggcaatacgc gtcgcgttgg tacgggatga tccaagagca
     8941 caactcaatt cagaaggctg aatactgtgg ttttcctgag actccagcgt aatcaacgcc
     9001 ataaacaacg tctcgttaat cccctgagcc ttcagcattt tattgcggtt ctccagcagc
     9061 ttgctttgca tgtgcatgca aagacgggtc agaaggatct cctgataagg aaaatcttcg
     9121 tggcggctgg cgcgaaattt tagcatttgt tcaatgggcg taaacgaact atccatttgg
     9181 gtatgacctc attaatttca gccgatatag taacgacagt gacaaataaa gtaaatgtat
     9241 tgttttagaa aaatgattct tgtgggttat ataatcgcca tcactttcca ggcgagccca
     9301 taggccagcg cactaagcag tgttgggatg ataatgctgc gtgttttata gaaactggca
     9361 cccagtaccg cgaagccgac cagagtgggc acgaaacggc gtgtatcgtg catcacttct
     9421 ggtgcggtag agacaaccag cagagcgcat atcgaggcga tgccaatggt gtcgagcaaa
     9481 atacctaccg cgccacgttt ggttgggcgg gcattaccca cacgcaggcg cagcggcaaa
     9541 tagcggaagc aataattcgc cgcgccaacc agtaacccaa gcagcagaac ctcatagctc
     9601 atcgggcgct ccttgccaga atgcctggat taacgcagtg aggcagccac agacaatgcc
     9661 tgccagaatg gcgacgggaa tagaaaatag cgttacgcct gcaagggcac caactaacgc
     9721 tgcggtaacg caaagagatt gtttgcgctg gaaagaggcg agcaggaaac tcataaagag
     9781 tgccggaagc ataaaaccta atgctgcttc aacggcggga tatccttgca gcaagccgct
     9841 gccggagaat gcccctatta ccgtaccaaa tacccacgat gaccatgaac tgaaggcaat
     9901 gccgatcatc cagttctcgc tccagcggcg attattgcgt accagttttg cggttgcggc
     9961 ggcaaaaacc tcatccgtca ggccaaacgc ccacagggca gttttcgatt tttgcagacg
    10021 ctgaataata cggctacgca gtgacgggcc atacaaaaca tggcgaacat ccattgccat
    10081 gacggtcagt gcagcaaccc acaaactact cccggctgcc agcatcgcgg taatgacgaa
    10141 ctggctcgcg cctgcataaa tgatgcagga gaaaaaaacg ctttcgagag gagagaatcc
    10201 cagacgggtc gcattcagac cgaacgcaaa ggccaccgga atataactaa taacaatcgg
    10261 taaactgtct ttgcatcctt ccatgaaggt cgccgaacca ggagcaggct gtggagtagg
    10321 gctttccata gagtgtacgc ttaacgattg ttaatattat aaatagactg aatgaatatc
    10381 ttaaccttat cagactgatg ggcttcttaa cacccttata agtgtaaagc cacgaaaacg
    10441 gttgctgatt gcttaaattg cggcttcctg acgatgaaaa cctcgccacc agaccagtaa
    10501 gttaaccagg gcaatcgtcg cgccagccag acaaacgcca gcccaaccgc catgttgcca
    10561 gactgaggct gaaattagcg aaccggcggc accgccaatg aagtagctgg tcatgtaacc
    10621 tgcggtcagg cgattacgcg catcagggtg tattcgataa attaccgtct ggttagtgat
    10681 atgcacgccc tgcacggtga gatccagcac caggatgccg ataatcagcg ccagtacgga
    10741 agtgtgacca aaccagatcg ccagccatgg aagtaacagc agcagcagac cgaaagttgt
    10801 ggtgtggtgc gatttgccct tatcggcaaa tccgcccgcc ggacgagcgc ccaacgctcc
    10861 ggctgctccc gcaagtccaa acagaccaat gacgccatcg ctgtaattaa aaggcggagc
    10921 ggcgagcaaa aaggccatcg aggtccagag aatgctgaag ttggcaaagg tcaggcagcc
    10981 cagcaacgtg cgggtgcgca gaattttatc gctgataaac atgctgaaaa cggaaccaag
    11041 caactgtggg tagttgaggt gggtttctga tttcatttgt ggcagaccac gccataatgc
    11101 cagcgccatc agtgccatta acaccgaagc aacccaaaag acggtgcgcc agccgccgag
    11161 gtttgccagc aacccggcaa cggttcgtgc cagcaagatc cccaacagca gcccgctcat
    11221 aatggtgccg accactttgc cgcgtttgtc cggtgaagcc agcgtcgctg ccagcggaac
    11281 cagaatttgt gccacgaccg agaataaacc ggttaatgcc gtaccgagga tcatcatcgc
    11341 cagcgactga ctgctggcag tgattaacat gccgcatgcc gccagtaagg tcatcgagac
    11401 aatcaggcgg cggcgttcaa acatatcacc gaggggaaca agaaacagca gacctgcggc
    11461 atagcccaac tgcgcggcgg taacaataaa gcctgccgaa ctggcggaaa gggaaaagtt
    11521 acgcgcgatg gtgtcgagca atggctgggc gtaatagtta ctggctaccg ccaggccggt
    11581 ggcgatagac atcagcacga tcagcgccgg gctaagctca tgattaggtt tagtcattat
    11641 ttttgttcat caggtgatga ttaagctgga aatcaataat agcggatgtt gatgaatatg
    11701 ggatgaaaga tagattgttg aaatgtgcgg tgtggtttgt aggtatgata agacgcacca
    11761 gcgtcgcatc aggcattgtg tacagagcgg cggatgaggc aaaaacgcct tatccgcccg
    11821 aataaaaatt acttctgcgc tgccagcgcc tcattcaccc agccatcgaa ctgctgctgg
    11881 tgggctttga tccagccatc aacgtggccc tgaatatcgc cttctgaggc tttgccgtca
    11941 tgcataatgg cgttctgggc gttaatatca gccactggta actgcataat ggcaaacagt
    12001 ttcgctgctg ccgggttttt ctcggcccag gctttgttgg caacgatatg catggtactg
    12061 accgggaagc cgtaattcgc gccattcggc agtttggtat cggcattttt atcgcccggc
    12121 agtgcggaga acggcacctg caaccagacc acatctttcc ccggtttcag ttcgttactc
    12181 acccagtacg gcgtccaggt gtagtaaaac accggcttac cctctttgta acgactgatg
    12241 gtgtcagcca tcatcgctgc gtagttcccc tgattatgcg tcacggtatt ggtcagtcca
    12301 tacgcggcaa gctggtggtt gatcgcacct tcgcagcccc agcctgggtt acagccggtt
    12361 aaatccgctt taccgtcgcc gttggtatcg aacagtttgg cgatcttcgg atctttaagt
    12421 tgcgcgatgt tggtaatttt gtactggtcg gcagttttct tatcgatcag gtaaccctgt
    12481 gccgcgccgt taacaaatac tccttcacga taaaatttct tatcgccacc ggcagcttcg
    12541 tacatgttgt catgcagtgg cgtccagttc actgcggtga aggttgcatc gccggaagca
    12601 agcgaggtgt agccaacgtt gtaatccact tcgctgggtt tattgacggt ataacctaat
    12661 ttctccagcg cgcggctgac cagcagcgtc tggaaagttt cttcggtgat ggtgctctga
    12721 accggattaa cagtaatgcc tttgcccggc aggtcggcag caaaagtttg tgtagagata
    12781 agcgtggcaa acgctgtcgc aaaaagtacg ctatgtcgca ttgttattcc tttttttggc
    12841 agggtgataa tgcccggcgt caccgggcaa gtgcagagtt acttaatgaa tgggcgggtc
    12901 agcagaccga cagggccagt ggtgtaccag cgacggttgc cgcgactgcg tgagtcgcgc
    12961 ccaacggcct gcgtcaggcg gtcgaggata atggcgagga tcacaatccc gacgccgcca
    13021 acggtggcaa gccccatatc cagacgaccg ataccgcgaa gcaccatctg acccagcccg
    13081 ccgacggcaa tcatcgaggc gatgaccacc atagaaaggg ccagcatcag cgtctggtta
    13141 acgcccgcca taatggtcgg catcgccagc ggtaactgaa ctttgaacag catctggcgc
    13201 gggctggcac cgaatgagcg cgaggcttca atcagatccg ccggaacctg gttaattccc
    13261 agaatcgtca gacggataat cggcggcagc gcaaagatga tcgtcaccac cacgcccggc
    13321 acgttaccga taccaaacag catgacgatt ggtaccagat aaacaaacgc gggcgtggtc
    13381 tgcatggcat caagcagtgg acgaataatt ttcgccgctc gcggacttct cgccagccag
    13441 atccccaacg gcaaaccgat gacgatacag aacagcaggg cggttagcac cagcgccaga
    13501 gtaaccattg cctgtgacca ggcaccgatt gcgccgatgg caatcagcga aaccagtgtc
    13561 gccacgccca ttccgacacc ggaaatttgc caggcgatga gagcgaaaac gataatcgcc
    13621 accggagcgg gcatacccag cagcaattgc tggaaaccgt tgaggatgta atcaaccgga
    13681 acgcgcacgc cctgaaagac gggacgaaaa tgggtaacga cccagtcgat cccttcagtg
    13741 acccaactgt cgagcgggat cagcgtttta tggaacggat cgagaatatt aaaatgctcg
    13801 acgtttggcg caggcgtact ggtcagccag tcagcaccac cgccgtcagt cggtgcagca
    13861 gccggtgtac cccaggcgtc tgcggactgt gcggcactgt ccgccgctgg cgtggtatcc
    13921 cacggattat tttgatcagc cattatttac cccctcacga tctaaagcgc gcagcagcat
    13981 tcctttcgaa atgatgccga catactgttg gtcctcatcg accacgggca ccgcacaggg
    14041 ggcctgtccg acatgagaga gcaactcgct aagaggcgtt tgtgcatcga ccgctaacgg
    14101 cgcatcaatc agcgccgcat caagaccttg ctgctgcgtt aacgcggctt taagcgaatc
    14161 gatagacaca gcgccgacaa acttattacc gcgttcgatg acgtagccat attcgcgatc
    14221 ttcatcctgc aataatttca gtgccgaacg cgggccgaag ccgggggttt tacgaattaa
    14281 gccgttcggt gtacgacggg caatatcttt cgcactgaac acctggctaa tatcgacgcc
    14341 acggaagaag gtacggacat aatcattcgc cggattattg agaatttcat ccggtgtgcc
    14401 gacctgtact acttcaccat tttgcataat ggcaattcgg tcgccaatac gcatggcttc
    14461 atcgagatcg tgggaaataa agacaatggt gcgctgatgt ttcgcctgta attttaccag
    14521 ctcatcctgc atctcggtgc gaattaatgg atcgagcgcc gagaaggctt cgtccattaa
    14581 taatatatcc ggattaatcg ctaacgcgcg ggctaatccc acacgttgac gcatcccgcc
    14641 agagagttca tccgggtagc tgtgggcata attttccagc ccgacctgac gcagtgcatc
    14701 aagggctttt tcacggcgtt cttcggcatt aattccggcc agttccatac cgaacgcagt
    14761 attgtccagc acggtcatat gcggcattaa ggcaaaggac tggaagacca tcgcaatctt
    14821 ttttctgcgc acctcacgga gttcggcgtc ggatattttg gcaatatcca caccatcaat
    14881 cagcacttgc ccgcgggtgg gttcaatcag gcgattgaga aggcgtacca ttgtggattt
    14941 acccgaaccg gataatccca tgatgacaaa tatctcgcct tcttcaatgg ccagactggc
    15001 gtctttaacg ccaagcgata gcccagtttt ttccagaatt tgttcttttg aaagtccttg
    15061 ttcgatatat ttgaaagctc gctgtggatg ctcgccaaat attttataaa gatttttaat
    15121 ttctaattta attgccatgc aatagaaaga ttcctgttat ttgtttatgt cgatacatt
//
LOCUS       DAZKGF010000120        15133 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZKGF010000120 DAZKGF010000000
VERSION     DAZKGF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.120"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1819)
                     /gene="flhA"
                     /locus_tag="ACHOQV_003507"
     CDS             complement(<1..1819)
                     /gene="flhA"
                     /locus_tag="ACHOQV_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFP9631818.1"
                     /translation="MSNLAAMLRLPANLKSTHWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPL"
     gene            complement(1812..2960)
                     /gene="flhB"
                     /locus_tag="ACHOQV_003508"
     CDS             complement(1812..2960)
                     /gene="flhB"
                     /locus_tag="ACHOQV_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFP9631819.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKSILVGSVTG
                     FYLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(3149..3793)
                     /gene="cheZ"
                     /locus_tag="ACHOQV_003509"
     CDS             complement(3149..3793)
                     /gene="cheZ"
                     /locus_tag="ACHOQV_003509"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFP9631820.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(3804..4193)
                     /gene="cheY"
                     /locus_tag="ACHOQV_003510"
     CDS             complement(3804..4193)
                     /gene="cheY"
                     /locus_tag="ACHOQV_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFP9631821.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(4208..5257)
                     /gene="cheB"
                     /locus_tag="ACHOQV_003511"
     CDS             complement(4208..5257)
                     /gene="cheB"
                     /locus_tag="ACHOQV_003511"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFP9631822.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAVGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(5260..6120)
                     /gene="cheR"
                     /locus_tag="ACHOQV_003512"
     CDS             complement(5260..6120)
                     /gene="cheR"
                     /locus_tag="ACHOQV_003512"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFP9631823.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNLHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(6139..7740)
                     /gene="tap"
                     /locus_tag="ACHOQV_003513"
     CDS             complement(6139..7740)
                     /gene="tap"
                     /locus_tag="ACHOQV_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFP9631824.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLESASAQSQRNYHISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(7786..9447)
                     /gene="tar"
                     /locus_tag="ACHOQV_003514"
                     /pseudo
     CDS             complement(7786..9447)
                     /gene="tar"
                     /locus_tag="ACHOQV_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
     gene            complement(9516..9806)
                     /locus_tag="ACHOQV_003515"
                     /pseudo
     CDS             complement(9516..9806)
                     /locus_tag="ACHOQV_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
     gene            complement(9827..11791)
                     /gene="cheA"
                     /locus_tag="ACHOQV_003516"
     CDS             complement(9827..11791)
                     /gene="cheA"
                     /locus_tag="ACHOQV_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFP9631825.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRDQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISPPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(11796..12722)
                     /gene="motB"
                     /locus_tag="ACHOQV_003517"
     CDS             complement(11796..12722)
                     /gene="motB"
                     /locus_tag="ACHOQV_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFP9631826.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(12719..13606)
                     /gene="motA"
                     /locus_tag="ACHOQV_003518"
     CDS             complement(12719..13606)
                     /gene="motA"
                     /locus_tag="ACHOQV_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP9631827.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(13733..14311)
                     /gene="flhC"
                     /locus_tag="ACHOQV_003519"
     CDS             complement(13733..14311)
                     /gene="flhC"
                     /locus_tag="ACHOQV_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFP9631828.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(14314..14664)
                     /gene="flhD"
                     /locus_tag="ACHOQV_003520"
     CDS             complement(14314..14664)
                     /gene="flhD"
                     /locus_tag="ACHOQV_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFP9631829.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         3530 a         4253 c         3689 g         3661 t
ORIGIN      
        1 ccagcggtgt atcgaggcca ataacatgga cttcatcttt gccaggaaac cactgctggg
       61 taatcgcccg gcccaacgct acgcgcacga cggcggttaa ttcatgcgga tcgctttgga
      121 tgggcgcatg ttccgccagc gtttcgagaa tggtgcgcat atcgcgaatc ggcacttttt
      181 catcgagcag attttgcagc actttatgca gtgtggtaag cgtgacgaca ccaggaacga
      241 gatcttccgt cagctttggc atctcctggg cgacgcgatc cagcagctgt tgtgcctcct
      301 gacgaccaaa cagctctgcg gcatgctggc taatgaggtg gttaagatgg gttgccacca
      361 ccgtgctggc ctcaaccacg gtgtacccct gaatctgcgc ctgttcttta agcgcacttt
      421 caatccagat agcattcagg ccaaatgccg gatcgacggt cgcctcacca ggtaacgtcc
      481 cggcagcggt accagggtta atcgccaacc aacgccccgg ataagcatca ccactgccta
      541 tctccacgcc tttcatcaaa atgcgatagc gggcaggttg cagatccata ttgtcgcgaa
      601 tgtgcaccac cggcggcaga aaccccatct cctgggcaaa tttcttgcga atactgcgta
      661 tacggcccag caactcacca tcctgctgga aatcgaccat cgggatcagg cgataaccca
      721 cttccattcc cagagaatct tccagttgca catcgttcca cgtcgcttcg acaacggtat
      781 tattctctgc catttttacc ggttttggtt cggcaggcgc tttttgttca cgtccgcgga
      841 tccaccaggc cagtcccagc aatccggcag tgaacagcaa aaataccagg ttcggcattc
      901 caggcaccag gccgagtaaa ccgagcacgg cggcgcttaa caacataacg cttgggttac
      961 tgaaaagctg attcaccatc tgctcgccaa catcctgatc ggtgctgaca cgcgtaacga
     1021 tgacccccgc ggcggtagaa atcaccaacg ccggaatttg tgccaccaga ccgtcgccaa
     1081 tagtcaatag cgtataactt tccgccgcgt gtcccatgct cataccatgt tgcagcacgc
     1141 cgaccagcaa cccaccgaca acgtttatga ccatgatgag gatcccggcg atggcatcgc
     1201 cgcgaacaaa cttacttgcc ccgtccatcg agccgtaaaa atcggcttcc tgagtcactt
     1261 cggagcggcg ttttttcgct tcatcttcac caatcaatcc ggcgttaagg tcggcgtcaa
     1321 tcgccatctg cttccccggc ataccatcga gaacaaagcg cgcgcctact tccgcaatac
     1381 gcccggcacc tttggtaatg accataaagt tgatgatcac gagaatgacg aacaccacga
     1441 taccgatagc gaaattgcca ccaacgagga agtgaccgaa cgcttcgacc acctttccag
     1501 ccgccgccgc gccggtatgc ccttccatca aaatgatacg ggttgaagcc acattaagtg
     1561 ccagacgcaa caacgtggta aacaacagaa tcgtcggaaa cgcagcaaac tcaagcgtgc
     1621 gttgggtaaa catcgccacc agcaacacca tgatcgacaa ggcaatattg aaggtaaaca
     1681 gcaggtcgag tatgaatgcg ggcagtggca gtaccatcat cgacaagatc aacaggatca
     1741 aaatcggtcc ggcaaggatc tgccagtgtg tcgatttcag gtttgcgggc aggcgcagca
     1801 tcgcggccag attactcatg ggtcggtttc tcgttaataa aatccagggc ttccggcacc
     1861 ggaagatgag taggttgtac agggcgctgt ccaccagcca gacgccagcg tttcagttgc
     1921 cagacccagg ccagcacttc cgccaccgcg gcgtacagtt gacccgggat ttgttgaccg
     1981 atctccgcat gtcgatacag cgcacgcgcc agcggcggcg cttcaagcgt cgggacgtta
     2041 ttttcagcgc caatttcacg aatgcgcagc gcgaccagcc ctgcaccttt agcgaccact
     2101 ttcggtgcgc tcattttgtt ttcgtcatac tgcaaagcca ctgagtagtg agttgggtta
     2161 ttgacgataa cgtccgcttt cggcacatcg gccatcatcc gccgccgcgc cgcagctcgc
     2221 tgcatctgac ggattcgccc tttgacatga gggtcgcctt cgctttgttt gaactcatca
     2281 cgaatatcct gccgtgacat acgcagcttt ttcaggtggc tgaagatttg gaaaaagacg
     2341 tcaaatccca ccatcggaat gacaccaagc accaccagca atgcgcacag ccctaccaga
     2401 tccatcgcat tacccatggc ggtaatgggg gactccgcca ttaagcgcat catttgcggc
     2461 cagtggtgcc agagataaaa ccccgtcaca ctgccgacca ggatggattt caaaatcgct
     2521 ttaagcaact ccgcaccggt ttgtgccgaa aacatccgtt taatgcccgg tagcgggttg
     2581 agtttggaaa acttcggctg caaggatttg ccgctaaata ccaggccacc caacataacc
     2641 ggagaaataa gcgccaccag caccacaccg ctaatcagcg gcagcagcgc cagcatggct
     2701 tctctgatca gcagaataat ttgcccgagg atcagattcg ggtcattgat aatactgtga
     2761 tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga cacaccgcca
     2821 aaccagataa cacacacgcc cactaacaaa atcagcagtg acgttaattc acgtgaacgc
     2881 gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc ttctgttttg
     2941 tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagcgt aaatgatgcc
     3001 agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt tagggctttt
     3061 actgccacac atcaagcata gtatgcgttt gtcggatgcg gcgtcatcgc ctgatatgac
     3121 gtggtcacgc cacatcaggc aatacaaatc aaaatccaag actatccaac aaatcgtcca
     3181 cctgatcctg actggctacc acaccggctt tgctggtatc gacctgaggt ccattaagca
     3241 aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc aacagcacca
     3301 tcaacaactg gcgttcgatc tcctgaatga catccatcat ccgcttaata acctgcccgg
     3361 tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta gtaaagctgg
     3421 tatgcgcggg gacatctgcc agaaattgcc gcgtatctgt taccagttct cgggcgtcgg
     3481 caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct tttgctgatt
     3541 tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc cgctccgcag
     3601 cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg atggcttccg
     3661 ccgcttcggc aatggcctga tccagcccca gttcccgcaa actgtcgcgc agcatacgcg
     3721 tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca ggtttgattg
     3781 atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt gttgagtttt
     3841 tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc cgcttgcgcc
     3901 gcagcaataa tgttctcttt cttcgcttct gcagtcacca ttaacactgg caatgctgac
     3961 atcgcgccat ccgcacgaat cgttttcagc aattccaggc catccatgtt gggcatgttc
     4021 cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc gtcgacacca
     4081 tcttccgctt cctcaacatt attgaatcct agctctttca gcaggttacg cactatgcgt
     4141 cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc catttcacac
     4201 tcctgattta aatacgtatc gcctgtccgg cactaatttt tgccagcatt tgctggctta
     4261 cctggctaag atcgaccact tcgcaaacac cacccatatt gatggcctcg cgcggcatgc
     4321 cgaacaccac gcaacttgct tcgttttgtg caagggtcca tgcccccgcc tgacgcatcg
     4381 ccaacattcc caccgcgccg tcgttgccca taccggtcag gatcacccca accgcattac
     4441 gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga tgacggttaa
     4501 ccgccgggcc atcgtgaatt ttgatttggt aatttgcgcc actacgcgcc agctccatat
     4561 gccgatcgcc cggcgcaata taagcatgcc ccggcaagac acgttctccg tcttcggctt
     4621 ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg gtgaaaccgg
     4681 gcggcatatg ctgggtaatt aacagtgccg ggctggaaag cggcaacggt tgcagtacgt
     4741 gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt tcagagctca
     4801 acaacggccc tgccttcagc gttgtcggtg ccgacaatgg cttatgtgct gcaaggctcg
     4861 cctttgctgc tgtacgcacc ttttcagcaa tcatttcgct atacgccagc attccttcgc
     4921 gaatacccag ttgcggtttg gtgacaaaat ctatcgcccc cagctccaat gcgcgcagcg
     4981 tgacttctga tcctttgccg gtcagggaag aaaccatcac aacgggcatt gggcgcaaac
     5041 gcattaattt ttcgagaaaa tccagtccgt ccatccgcgg catttcaaca tccagcgtta
     5101 gcacatccgg attgaatttc ttaatcaaat cacgcgcaac cagcggatca ggggcggtcg
     5161 ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg cgcatcagtg
     5221 ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac ttagcgcata
     5281 caccgtctga ccacgcagcg tgaagcggcg ctcaaggtgg ctaaagtttt cagagtgacc
     5341 cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa tctcctgctg
     5401 ggtagtttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa acggccccgg
     5461 cacggtgtac tgtttcgcca gtaaattcag cggggcaaaa tcaacatagt tcgccagctc
     5521 ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat accgttgcag
     5581 ttgctgcggc gtcaggtttt tcaactcttc atggcgataa ataccgcttc tggctttttc
     5641 cagcacttcg gtgtcgatat cgctggcaaa cactttccag cgtccgggca cggtgcccaa
     5701 tgtgtcagcc agcgtcatcg caatgctgta cggctcttcg ccggtcgaag ccgccgcgct
     5761 ccatacgcga tactcgccag aacggcgacg tgcgtgatcc gcgagcagag gaaaatgatg
     5821 cgcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg cctgccactc
     5881 accgctgtgc agattagatt ccagcaagtt caggtaatga ccgaaatccg tcagtcccag
     5941 cgaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt cggccaggac
     6001 gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt cggaaagcgc
     6061 caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag atgaagtcat
     6121 agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg ccgactcatg
     6181 tcgtgctact tcatgctctt caagggtaaa taccgccacg cgcgacgaaa gatggtcggc
     6241 ctgattcgcc agttgttctg ttgccaccgc cgcttcttct accagggagg cgttctgctg
     6301 tgtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc gccgttgttc
     6361 ttccgaggcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg aactgacaat
     6421 atcagtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga cacgattgac
     6481 tgactcttcg atcagccctt tgatctcttt tgctgcctgg gcgctacggc tggcaagatt
     6541 gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc gagccgcttc
     6601 cactgccgca ttcagggcca gaatattggt ctggaaagca atgccgtcga taacgctgat
     6661 aatgtcgcca attttttgcg agctggtagc gatctcctgc atggtgtgag tcatggtact
     6721 gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt ccgacgcctg
     6781 tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac tggcggccgt
     6841 ttgtgccagc gatgccgcct gctgttcggt gcgacttgag agatcgttat tgcctgcgac
     6901 aatctccgcg ataccaatgt gcatctcctg gcttccctta cgcacatcac ttaccgtccc
     6961 acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct cattgcgacc
     7021 atacaccgca atcggacgtg ccagattacc cgcagcaatg ctgtcaaaat ggctaccgat
     7081 aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc tgatgtagat
     7141 cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atgtgatagt tacgctggct
     7201 ttgcgcactg gcactttcca gcacatggtt gatctccagc tgccaggcct caaagttaac
     7261 gtcaaacgca ttctgtgatt cctgcaccgg cgcagtgagg aaatccgaaa gttgattact
     7321 ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac ttttctccgt
     7381 ctctttttgc aacgccctga cgtgctcgtt gcccgcagtc atcgccataa aacttttaaa
     7441 cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt taatgtcatc
     7501 cgccggatag ctaagcgccg tcagagtgcc cgctttgttc agcgcggtac tggcctgtaa
     7561 cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct cgacctgatt
     7621 cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc caatctgcaa
     7681 gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac gattaaacat
     7741 caacgctccc catcaggcgg caatgaccgc gttagtaaat actcgtcaaa atgtttccca
     7801 gtttggatct tgttcagcaa ttcgcagtcg tggctgtgcc ggtggttgct cactggcagg
     7861 acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga acgcggaaac
     7921 cgcttgcgtt aaacgactcg cctgttcttc cagcgcagct gcggcggcag ctgattcctg
     7981 caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca atgcgacttg
     8041 atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa tgtcagtcac
     8101 gcgggtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga ccagcaccga
     8161 accggtatca acgcgcgaga cggagtcttc aatgagggct ttgatctctt ttgccgccta
     8221 ggcgctgcga ctggcaagat tacgcacttc gcccgccacc acggcaaaac cacggccctg
     8281 ttcacccgct cgcgcggctt caacagcggc attcagcgcg agtatattag tctggaaggc
     8341 aataccatcg ataacgctga taatgtcggc aattttcttc gaactatcgg cgatctcatg
     8401 catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt cggaggcact
     8461 ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg tcgcggtgag
     8521 ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttccg tacgggagga
     8581 aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat ctgaaccttc
     8641 acgcacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc tctgcgccag
     8701 gtcgcccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac caccggcgat
     8761 ttcgcgaatg tgagcaataa tttttgccag cggtgtaagc aacatacggc gaatgccgta
     8821 ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt gccactgggc
     8881 aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt cactgctgag
     8941 ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg gctgagcgaa
     9001 ataagctcca gtattgccat aatcaagata atcaatcagt tcagttaacg ctgtgtgata
     9061 gtttttatat ttttcatcaa tattacgact ggtagcgacc atttcaggta acggtgccat
     9121 gcttttgaat tttttataat gagtcgctgc ctgggccaat gttttcctgg cgctatcgag
     9181 caattcaact ttggcattac tttgctgatt ggatgaatcc atcatcatcc gcaccgctga
     9241 acgactcagg ttaatgcgcg tttgcagcat taaatcccat gttgacgtca gctcgccctg
     9301 ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa gggaagaaaa
     9361 aaacagactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca ttaccaacag
     9421 cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt tgatctcgtt
     9481 atcggcaagg aagggggaaa ctttattgct gatgccaccc gctgtccgag attcaggacg
     9541 ataactaccg tgttgtcgtt atagtccaca tccacctggc tgaacttaat tcgtaagtcc
     9601 acaatcggca caataacacc gcgcagattc gtgacgcctt tgataaacgc tggcgtgttc
     9661 gcaatccgtg ttacctgatc gtagccacgg atctcctgca ctttcagaat atcaataccg
     9721 tactcttcat caccaagggt aaataccaga aattcctggc ctgacggctc gctggccagc
     9781 tttgttacat tcgtcatacc ggtcatattg ttaccttttt actcattcag gcggcggtgt
     9841 tcgccatacg ttgttcgcgg tttatcgcct gcaaggcgga gacatcaaca atcagtgcca
     9901 cgctgccgtc gccaagaatg gtcgcagcag aaatgccggg gactttgcga tagttacttt
     9961 caaggttttt aaccacaacc tggtgttgac caattaattg atccaccagc aaggcgtagc
    10021 ggcgaccgcc actttgtaag atcaccacaa ttccctgggt agcttcggtt ttcgcgcccg
    10081 cgacgttgaa cactttccac agttcgacga tgggcagata ttcaccccgc acttccagca
    10141 cccgctcgcc gccagccagt ggatgcagat cggcttcacg gggttgcagt gattccatga
    10201 cagcattcag cggcagaatg aaaacttcat ccgcaacgcg tacggacatg ccgtcgagga
    10261 tggccagcgt cagcggcagt aaaatgcgga tcgtcgtgcc agtaccctgc ttcgactgga
    10321 tttcgacatg accgcccatc tcctggatat tacgtttaac gacgtccatg ccgacgccgc
    10381 gcccggagac gtcggtgacc tgctccgccg tggagaagcc aggggcaaat atcagcatcg
    10441 cgacttcgtc gtcgctcatg ttttcgctga cggtcagacc ttgcgaggcc gcttttgcca
    10501 gaattcgctc acggtttagc cccgcaccat cgtcggtcac ttcaatgcaa atgttgccgc
    10561 cctgatgttc ggcagacaga attaaatttc cgacgctgtt tttacctgcg gcgagccgtt
    10621 tttctggcag ttcaataccg tgatcgaggc tattgcgtac caggtgggtc agcgggtcga
    10681 taatgcgttc tatcaggctc ttgtcgagtt cagtggaact gcccaccagc gtcagttcta
    10741 cctgcttgcc gagttttccc gcaagatcac gcaccagccg gggatagcga ctaaagacat
    10801 actccatcgg catcatgcga atcgacatca ctgattcctg caaatcacgg gcgttacgtt
    10861 gtaactgccc catgctggta atcaaatcac catgattaac cgggtccagt tcactagaac
    10921 gctgggcaag cattgactgg gtgatgacca gctcgccgac gaggttaatt aattgatcaa
    10981 ccttttctac cgctacacgg atgctggtgg attcactgct gcgcgtcgtt ttttcccgct
    11041 ccacgcggcc ggttggcgct tgttcggctg ccagtttaag cactggtggg ggggatattt
    11101 tgggcgagac ttctactgtt tcaaaggtaa tctgatcggc ttcaatcaca aaacagagta
    11161 ccgctgtgat gtcatcttcg gcgatgtcgc ccggtaatat tgccgagaga gaatccgccc
    11221 ctttcaccac atcagttaac gttgtcagat gccccagctc ttcttccagc aggtcgactt
    11281 ccccggcctt caggcgcgaa aggataattc gtcgcggcga ctgatcgcgg ctctgctcat
    11341 cttgcggttc acttttggca accacactta atcgggtcac tgcagatggc gtttcgcctt
    11401 tcgcttctaa tgccagttga cgcaaggcct ggcagatata atcgaagctg gcggcatccg
    11461 gctcttgcga ctgtttataa gcgtcgagct gttcttgcat gatgtccttc gtttccaaaa
    11521 acagattgat aatgtcggtg ttgagttgca tctcacctcg tctggcttcg tcgagcaggt
    11581 tttccatcag atgcgtggtt tcctgcaaaa cgctgaagcc aaaggttcct gcccctcctt
    11641 tgatcgagtg ggcagcccga aagatggcat tcaattgttc ggcatctggc gcttccggct
    11701 gcaaaaccag cagatgctgc tccatgtcag ccaacagttc gtccgcttca tcaaaaaatg
    11761 tctgataaaa atcgcttata tccatgctca cgctgtcacc tcggttcggc tgatggcatt
    11821 gtgggaacac tgacctgtgg tgcaacctca ggtttttcca gggcgcttac tggctcattc
    11881 tggctttcgg cgttttcatg caaaatggcc tgttcggctt gtttgttcag taccagcagg
    11941 ctgatgcgac ggttgacggc atcatcaggt ccgcgatcgc ttaagcgcat cgttgccgcc
    12001 atgccgacga cacgtaacac tttgccgcca ttcaacccac caaccatcag ttcgcggcgg
    12061 gatgcattgg cacgatcggc agaaagctcc cagttgctgt aacctttctc accgctggcg
    12121 tagggaaaat catcggtatg acccgagagg ctaattcggt tgggaatacc gttcagtacg
    12181 ggcgcgatgg cgcgcagaat gtcgcgcata tagggttcga cgtcggcact gccagttcta
    12241 aacatcgggc gattctggct atcgatgatc tgaatacgta gaccttcctg gaccagatcg
    12301 attttgagat gtggacgtaa cgcccgcagt ttcggatcgg actcaatcaa ctggtcgaga
    12361 tcgccacgca atttccgcaa tcgactttgc tccatgcgtt ttttcagctc ttcgatgttc
    12421 ggctgcttat tcacttcccc ctggctttgg gtgtaatcat cacccccacc gggaattggg
    12481 ctttcactat tagaaatgcg atcgccgccc gtaaccgcag tcgccagtgg agtccggaag
    12541 tactccgcaa tctgaatcag ctcttttggg ctggagatgg agattaacca catcaccaga
    12601 aaaaaggcca tcatcgcggt cataaagtcg gcataagcaa tcttccacga tccatgtgct
    12661 gccccgtggc ttttggcttt gcgtcgtttg acgacaataa tcggatgcgc ttgattcttc
    12721 atgcttcctc ggttgtcgtc tgttgttgcg gatttttcac cgcacgcaca tgctcttcca
    12781 gttcaataaa cgacggacgt tcgctggaat agagcgtttt gcgaccaaac tcaacggcga
    12841 taggcggtgc gtaaccgttc agattagata gcagtgtgac tttgacgcac tgcatcattt
    12901 tgctggtttc ggcgcttttc tgacgtaaaa cactcgctaa tggggaaata aatccgtaag
    12961 ccaataaaat accgaggaaa gtccccacca tcgcatgtgc gataagcgcc cccagctcgg
    13021 cggcaggacg atcggctgaa cctaacgcgt gaacgacccc cattacagcc gcaacaatac
    13081 caaacgccgg aagtgagtca ccgaccagcg ccagactgtt cgccgggact tctgcctcgc
    13141 tttcgtgcgt ctcaatctct tcatccatca gagcttcgat ttcgaaggtg ttcatgtgac
    13201 cgctgataat caggcgcaga taatcgacga taaattcaag catgacgcta tccgcaagga
    13261 tgcgtgggta gctggcgaag atctcgctct cacggggatt ttcaatatca cgttccagcg
    13321 aaaacatccc catctgccgc gatttcgcca tcaaccgata aagcagagcc agcagatcca
    13381 tatacattgc tttggtgtat ttggagcgac gaaacagcaa cggcagcgcc ttcagcgtgc
    13441 ctttaatcgc tttgccatta ttgccgacga taaacgaccc aatccctgca ccggcaataa
    13501 tcaccagttc agcgggttga tagagtgctc caaggcttcc accggtcatc aaataaccgc
    13561 cgaaaactgt accgagaaca accaggtaac ctaataagat aagcacgaca tcatccttcc
    13621 actgttgacc atgacaggat gttcagtcgt caggcgttaa cacgcgatag gggcaaaaaa
    13681 aagcagcggt acgtcgttac cgctgctgga atgtttcgcc acaccgtatc agttaaacag
    13741 cctgtactct ctgttcatcc agcagttgtg ggataatatc ggcaggattc tgggaaagtt
    13801 tacgtctttt tactgcccgg gatggcggct gacataagct gcaggcaaag ctgccaacag
    13861 gctggtgagc gtgggtaata aaattgccgc cgcagcagtt gcagctggaa agttgcagta
    13921 atccactttc aacaaaccgc accaatgtcc aggcacgggt taatgccagc agcggtccct
    13981 cttctgcttg cgggcactgt tcaaggtata aacggtaggc tttgatcacc gcatcgacgc
    14041 cattacacaa accggttttc agtaaaaact gccatgcgtt acagaacatc gaagcatgaa
    14101 cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg cagcatgcct ttcggcggtg
    14161 ggcttccgcg cagttcttta tagagtttta tcaggcgtcc gcgacttaac tgtgtttcgc
    14221 tttccagcat ctgcaaacga gcgcccaggg tgatcaattc cattgccagc tgaatatccc
    14281 gcgcttcctg aacaatgctt ttttcactca tgatcaggcc cttttcttgc gcagcgcttc
    14341 ttcaggctga ttaacatcat tcagcaagcg tgttgagagc atgatgccgg tatgaatttg
    14401 ctggagatcg tcaacgcggg aatcttgcgt caactgagta atcgtctggt gactgtcaaa
    14461 acggaagtga caaaccagtt gattggtttc tgccagctta accatttgcg gaagagtcag
    14521 tgccgctaac gttgtcgcca tttcttcatt tatgccgaga cgaaacatag cggacgcttt
    14581 gtcctgaaca atcagacgct gtgcaagtag taaatatgac aagttgatgt cataaatgtg
    14641 tttcagcaac tcggaggtat gcattattcc cacccagaat aaccaacttt atttttatgc
    14701 ggtctcaccg caccccgtga tgtcgccggg aagccccggt aaaaaataat tagcattaga
    14761 atagttgcga taagctgcaa taggcagagc ccctttgtgg gatttaataa gtccttacaa
    14821 atagaagggg ttctttacac ttatctaaga tttttcctaa atcgacgcaa ctttactcgt
    14881 cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg atgcagatca
    14941 cacaaaacgc tcaattactt aacataaatg tataagtcat acttttgttt tgggtgtatt
    15001 tcaatctgtt aaaaagtttt tcgcgacgct agcaagcaaa aatgaaacag gaataataga
    15061 aatgggatgt tgcgcacagt taaaataact caccgtaaat aatcatctgc tataaataat
    15121 tgctttaatg caa
//
LOCUS       DAZKGF010000121        15082 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZKGF010000121 DAZKGF010000000
VERSION     DAZKGF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.121"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(25..237)
                     /gene="cspA"
                     /locus_tag="ACHOQV_003521"
     CDS             complement(25..237)
                     /gene="cspA"
                     /locus_tag="ACHOQV_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFP9631830.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(518..808)
                     /locus_tag="ACHOQV_003522"
     CDS             complement(518..808)
                     /locus_tag="ACHOQV_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFP9631831.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1242..1952
                     /locus_tag="ACHOQV_003523"
     CDS             1242..1952
                     /locus_tag="ACHOQV_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFP9631832.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2002..2976)
                     /gene="ghrB"
                     /locus_tag="ACHOQV_003524"
     CDS             complement(2002..2976)
                     /gene="ghrB"
                     /locus_tag="ACHOQV_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFP9631833.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTIEQNAAILA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3080..3739)
                     /locus_tag="ACHOQV_003525"
     CDS             complement(3080..3739)
                     /locus_tag="ACHOQV_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFP9631834.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3892..6225
                     /gene="bisC"
                     /locus_tag="ACHOQV_003526"
     CDS             3892..6225
                     /gene="bisC"
                     /locus_tag="ACHOQV_003526"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFP9631835.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVMVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMLVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6194..6634)
                     /locus_tag="ACHOQV_003527"
                     /pseudo
     CDS             complement(6194..6634)
                     /locus_tag="ACHOQV_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(6631..7194)
                     /gene="tag"
                     /locus_tag="ACHOQV_003528"
     CDS             complement(6631..7194)
                     /gene="tag"
                     /locus_tag="ACHOQV_003528"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP9631836.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRASFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            7352..8050
                     /locus_tag="ACHOQV_003529"
     CDS             7352..8050
                     /locus_tag="ACHOQV_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP9631837.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8279..9487
                     /locus_tag="ACHOQV_003530"
     CDS             8279..9487
                     /locus_tag="ACHOQV_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9631838.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            9811..11502
                     /gene="eptB"
                     /locus_tag="ACHOQV_003531"
     CDS             9811..11502
                     /gene="eptB"
                     /locus_tag="ACHOQV_003531"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFP9631839.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            11594..11670
                     /locus_tag="ACHOQV_003532"
     tRNA            11594..11670
                     /locus_tag="ACHOQV_003532"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11628..11630,aa:Pro,seq:cgg)
     gene            11877..13124
                     /locus_tag="ACHOQV_003533"
     CDS             11877..13124
                     /locus_tag="ACHOQV_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFP9631840.1"
                     /translation="MARKTTPLTTTQIKAARPGEKELTLHDGGGLYLLIKPSGAKIWR
                     FSYYRPVDKKRALMSLGSLDEIPLAEARRLRNEYRSLIASGTDPQDHERRKREAEARR
                     QGNTFENVAAAWYQMKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTAHRFVEIL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDSNPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMLAVAGASIERQTRLLIEWQLLTVTRPAEASSARWEEIDLKSSTWTIPAGRMK
                     MRRDHVIPLCDQAMAVLEAMKPISHHREYVFPGLKDPRQPMNSQTANVALRRMGFGGI
                     LVSHGMRAIFSTAANEEGFEPDVIEAALAHVDMNSVRRAYNRSNYLEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAVQN"
     gene            13259..13486
                     /locus_tag="ACHOQV_003534"
     CDS             13259..13486
                     /locus_tag="ACHOQV_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631841.1"
                     /translation="MQDTQHNSEGMQLALLVNREFWSTYDPEDKSTAPSSREVIDFLV
                     SRGASKNLAVSIDKVVRPASMKAGGRPRKWR"
     gene            13764..13970
                     /locus_tag="ACHOQV_003535"
     CDS             13764..13970
                     /locus_tag="ACHOQV_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9631842.1"
                     /translation="METIRKIIVRTEVKKLINIKSDSTLQAMIKAGEFPAGFRIGLRR
                     RGWYEDEVLAWLKEREQEARGAVA"
     gene            14110..14382
                     /locus_tag="ACHOQV_003536"
     CDS             14110..14382
                     /locus_tag="ACHOQV_003536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631843.1"
                     /translation="MKRPNMPVQGQGFVQPEISQNDILIRHKAESRIDSRVISERAGI
                     QHESIIATIESHQSRLRELGSLPRQSVSQGINLFEIGKTWPQARSS"
     gene            14342..14833
                     /locus_tag="ACHOQV_003537"
     CDS             14342..14833
                     /locus_tag="ACHOQV_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631844.1"
                     /translation="MKSGKPGRKRGRPEISYLLNELQLDYLLRVIRGRDAERINQFKL
                     DVTKAFAKHRAAEPVRREYLPGYHESRDGLKALGAERHHYINLARAENRVTGLVAGER
                     STASEQQLGLLIVMQKIEQAAFDEAIQSGMTPTEAVREVARRMETFASLMTSASSLGV
                     KHA"
     gene            14826..>15082
                     /locus_tag="ACHOQV_003538"
     CDS             14826..>15082
                     /locus_tag="ACHOQV_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ash family protein"
                     /protein_id="HFP9631845.1"
                     /translation="MLKNFAELTPERVEFMRHIVSHFAAERMKHVKQYLEEVLSNYRA
                     GGPVRREYLHDAGMAGKTKATTEGSPVERKEIKRIIHNAIS"
BASE COUNT         3704 a         3653 c         3931 g         3794 t
ORIGIN      
        1 agattctgtg cttttaagca gagattacag gctggttacg ttaccagctg ccgggccttt
       61 agcgccgctt tcgatggtga aggacacttt ctgaccttcg tccagagatt tgtaaccatc
      121 gttctggata gcagagaagt gtacgaacac gtctttagag ccatcgtcag gagtgatgaa
      181 gccgaagcct ttgtcagcgt tgaaccattt tacgatacca gtcattttac cggacatagt
      241 gtattacctt taataattaa gtgtgccttt cggcgatatg gcgtgcttta cagattttga
      301 agcgttaaag gaatgtgcac tacgaggggt atcaacgata actcttgaag ggacttgcct
      361 tactacactg ctttaatggt ctgtacgtca aaccgttgat gtgcattaag ccacgcattg
      421 gcgggtgatg caacaattat ttttcatatt tatgattaat cggttggaaa acggtcctgt
      481 catcaggacc gtaaacagca ataaagtgga taaaagtcta ttccatcaac tgcttactta
      541 atgccgggtt ggcttgaatc aaacgcatca attttagttc ggcacttgaa ggcttcacgc
      601 gtctggattc ccattccttt accatggcga ctgatacgcc caaaacccgg gcgaaatcat
      661 cgatttttaa ccctgtccct tttcgtaact gctcaatttc ggtacaggac gttgttctgt
      721 gcgtcagggt aattttctgc gtttcatctt taaaaacaat ctgttccagg ctgctcaaca
      781 gctcatgcat tggatcttta tattccattg aaaactcctc aaatcacact gcgggatcgt
      841 gaattgcatc gaagctcatt aagaatagtc gggaaaaaca gacatagcgg ggtgtagtca
      901 gggcatgctc tggctatgat tcgattcagc cagtcattta gcgtgactta tagccaccta
      961 acgatctgat aaatgccgat atatctctta ccactcccgg tttgtaaaga tttcgaaaat
     1021 ctaacgttct gcgaaggcgt aaatctcagg ctacaaaaaa agcaacggca ctatcatttt
     1081 ttaacaatga aacaacaaat tgaccttgtt tcattttctc catgatttta ccaggaagat
     1141 tacgtagaaa gggtatcctg caagcctgtc ctggactatc cttgtcacgt cagacacgcg
     1201 tgtgtcgttg tgcgcttttt ttgggtgaaa ggagtaagaa aatggcgaca ggaaagtcct
     1261 gctctcgctg gtttgcgcct cttgcggcgt tattaatggt agttagcctg agtgggtgtt
     1321 ttgataaaga aggcgatcag cgtaaagcgt ttatcgactt cctgcagaat acagtgatgc
     1381 gtagcggtga acgtctacca accctgactg ccgatcagaa aaaacagttt ggtccttttg
     1441 tctctgatta cgcgattctg tatggttatt ctcagcaggt gaatcaggcg atggattccg
     1501 gtctgcgccc ggttgtagac agcgttaacg caattcgcgt accgcaggat tacgttacgc
     1561 aaagcggccc actgcgtgaa atgaacggtt ctctgggcgt actggcgcaa cagctgcaaa
     1621 atgcgaagtt gcaggccgat gctgcgcact ctgcgctaaa acagagcgat gacctgaaac
     1681 cggtcttcga tcaggcattc accaaagtgg taacgacgcc agctgatgcg ttgcaaccgt
     1741 tgattccggc ggcacaaact ttcacgcaac aactggtgat ggttggagac tacattgctc
     1801 agcaaggtac tcaggtgagc tttgtcgcaa atggcatcca gttcccgact tcgcagcagg
     1861 caagtgaata taacaaactg attgcgccat taccggcaca acatcaggcg tttaatcagg
     1921 cctggactac agcagttact gcaacccaat aaagagtaaa agcccgagcg attatcgggc
     1981 tttactccac gcagtcgcgg cttagtccgc gacgtgcgga ttcacacagt tcttctcaac
     2041 ctttccttgt aacgcatcaa tcaagttatc cacggcacag gcggccatgc cataacgcgt
     2101 ctcatgagtg gcagatccaa tatgcggtac tgcgacgacg ttggccattg agagcaacgg
     2161 cgaatctact gacagtggct cttgttcgaa gacatccagc ccggcagcgt gaatttcccc
     2221 tttctgcaat gcagcaatca gtgcattttc atcaaccacc gggccacgtc cggcattaat
     2281 gaaaatggcg gaggatttca ttttggcgaa ttgttctgcg ccaaacagat gatgcgtttc
     2341 atcagttaac ggcaggatca ggcaaacgaa atctgactct tgtaacagtg tatccaaatc
     2401 gcagtagcgg gcgttgaagc gttcttctgc ttctttatgg tggcggcgcg cgttatagag
     2461 gatgggcatg ttgaagccaa agtgcgcacg ttgtgccagc gccatgccga tccgtcccat
     2521 cccgacaatg cccagtgttt tatggtgaac gtcagtgccg taccagtccg ggcctatgct
     2581 cgcggtccat tcgcctgctt ttactcgttc tgctacctcc acaacccgac gagcggtaga
     2641 caataccagc gccatcagcg tatcggcgac ggtttctgtt aatacggttg gcgtgtgcat
     2701 cagcagaatt tttcgggcgg taagcgcatc gacatcaaaa ttgtcatagc ctacggagat
     2761 cgttgatgtg gcacgcagtt tcggcatttt ttccagcaat gcggcatcaa cattctcgtt
     2821 tgaacccagt aaaccttcag cttcggcaag aattgctgca ttttgttcga tggtttgtgg
     2881 gctgaggttt gccacctggt gaacggtgaa atgctcttgc aggcgttgca gtaaatcatc
     2941 aggtaaggct ttgtagagga taacggacgg cttcattctt ctctccattc actgataagg
     3001 cgatcacctt agcctgaaat atgatgaagg aaaactgaag aatgccaggt gatttctgca
     3061 tcacctggca tgaaagggat tacagcgggc ttaaggtaat ttctacacgg cggttttgcg
     3121 ccttaccttc tgcagtgctg ttgctggcga ttgggttagc cgggccaagg ccctgagtac
     3181 ggatgcggct ggcgtccacg ccctgggtga tcaacgcgct ggcaacggaa tccgcacgct
     3241 gctgggagag acgcatgttc aggtcgtgcc cacccgtgct gtcggtataa ccaatcacgt
     3301 taaccgccgt tttcggatac tctttcagta ccattgccac gccggtcagg gtgttagcgc
     3361 ccgccggttt cagggttgcg ctgctgctgt cgaaggtcac attgttcggc atattgagga
     3421 taatgttatc cccgctgcgg gttacgctaa caccagtgcc gcgcattttg tcacgcagct
     3481 tcgcttcctg cacatccatg taataaccca cgccgccgcc cagagctgcg cctgctgctg
     3541 cgccaatgag cgcgccttta ccgcgatctt tcttcgaaga agagagcgca ccaatacccg
     3601 cgccaacgag agagcccaga cctgcgccga tagcagattt acctgcttcg cgttcgccgg
     3661 tgtaagggtt agttgtacag ccagataccg ccagagcgcc actcactacg gcggcaataa
     3721 gataaacacg tttcttcatt gttaatcctt aataaccttt ttattctttg ccacgggttc
     3781 tgtggcggga gattatgccg cgtgaacatg aagattattc ctgggaatac tcggaaattt
     3841 gtaagtaata tttaactgct caatacatct aacctttcag gagtcttcgg tttggccaac
     3901 tcatcctcac gatattccgt tctgactgcc gcccactggg ggcctatgct ggttgaaacc
     3961 gacggcgaaa ccgtgtttag ctcgcgtggc gcgttagcca caggaatgga aaactccttg
     4021 cagagcgcgg ttcgcgacca ggttcacagc aatacgcggg tgcgatttcc aatggtgcgc
     4081 aaaggctttc ttgcgtcacc ggaaaatccg caaggcattc gtgggcagga tgaatttgtt
     4141 cgcgtgagtt gggatgaggc gctggagctt attcaccatc aacataaacg cattcgtgag
     4201 gcttatggtc cggcatcgat ttttgctggt tcctacggct ggcgttcaaa cggcgtgctg
     4261 cataaggcct cgacattatt acaacgctat atggcgctgg caggcggtta tactgggcat
     4321 ctgggggatt attcgaccgg cgcggcacag gcgatcatgc cgtatgtcgt gggtggcagc
     4381 gaagtttatc aacagcaaac cagttggccg ctggtgctgg aacatagcga tgtcatggtg
     4441 ctgtggagtg ctaacccact caatacgctg aaaattgcgt ggaatgcatc cgatgagcag
     4501 gggctttctt acttttccgc actgcgtgac agcgggaaaa agctgatctg cattgatcca
     4561 atgcgatcgg aaaccatcga tttctttggc gataaaatgg agtgggtggc accacacatg
     4621 ggcaccgatg ttgcgttgat gctggggatc gcccatacgc tggtggaaaa tggttggcac
     4681 gacgaagcgt ttctggcgcg ttgcaccaca ggttatgccg tcttcgcctc ttatttgctg
     4741 ggcgagagtg atggtatagc gaaaaacgcc gaatgggcgg cagagatttg tggtgttggc
     4801 gcagcgaaaa tccgcgagct ggcggctatt ttccaccaaa atacaaccat gctgatggca
     4861 ggctggggaa tgcagcgcca acagtttggt gagcaaaaac actggatgct cgtcacgctg
     4921 gcggcaatgt tggggcaaat cggcacaccc ggcggcggtt ttggtctttc ttaccatttt
     4981 gccaatggtg gtaaccccac gcgccgcgct gcggtgctct cttccatgca gggcagtttg
     5041 cctggcggca ccgatgcggt ggataaaatc cctgttgccc gcattgttga agcactggaa
     5101 aaccccggtg gcgcatatca acataacggt atggaccgac atttcccgga tattcgtttt
     5161 atctggtggg cgggtggtgc caactttact catcatcagg ataccaatcg cctgatccgt
     5221 gcctggcaaa aaccggagct ggtggtgatc tctgaatgct tctggacggc ggcggcgaaa
     5281 cacgcggata tcgttctgcc tgcgaccacc tcttttgagc gtaatgatct caccatgacc
     5341 ggcgattaca gtaatcagca tctggtgccg atgaagcaag tggtgccgcc acgctatgaa
     5401 gcgcgtaatg attttgatgt ttttgccgag ttaagtgaac gttgggagaa gggcggctat
     5461 gcgcggttta cggaaggaaa aagtgagctg caatggctgg aaacgtttta taacgttgcc
     5521 cggcaacgcg gggcaagcca gcaggttgaa ttgccgccat ttgctgagtt ctggcaagcc
     5581 aaccagttaa ttgagatgcc ggaaaacccg gacagcgagc ggtttattcg cttcgccgat
     5641 tttcgccgcg atccgcaggc gcatccatta aaaaccgcca gcggcaagat tgaaatcttc
     5701 tcgcagcgta ttgccgatta cgcttatccg gattgccctg ggcatccaat gtggctggag
     5761 ccggacgaat ggcagggcaa tgccgaaccg gaacagttgc aggtactttc tgctcatccg
     5821 gcacatcgtc tgcacagcca gctgaattac agttctctgc gcgaattgta cgcggtggca
     5881 aaccgtgagc ctgtcaccat tcatcctgac gatgctcagg cgcgcggcat acaagatggc
     5941 gatactgttc ggctgtggaa cgcacgcggg caaattcttg ccggggcagt cattagcgag
     6001 ggaattaaac ctggcgtgat ttgcattcac gaaggggcat ggccggatct ggatttaacc
     6061 gctgacggta tttgtaaaaa cggcgcagtg aacgtgctga ccaaagatct ccccagctcg
     6121 cggctgggga atggctgtgc gggtaatacg gcgctggcat ggctggaaaa atacaacggt
     6181 ccggaactga cacttacagc gtttgaacca ccggccagct cataatccag gtgggtagtt
     6241 gggtttcatc ctgccatgcg cagtcgacaa tgtgaaaacc ctgcgcctgg taaaaatcta
     6301 tcgccggttg atttttttga taaacctcca gcatcagatg gggatggcgc tgctgcacat
     6361 actgcattag cgccttacca ataccgcgtc tgacggcctt cggtgcgaca aacattgcag
     6421 ccagaaatcg gccttccata atgctgacaa aaccgagaag cttaccgtct tcttcccaga
     6481 cccagttttg cgcgttggca agatacgcat cgcgcaccag cggaatgcag tcacgccagt
     6541 aactcgattt tataaaggga tgcccctagg ttgtactttc cagccacagt tcgaggatcg
     6601 cggggagttc tgaatgttgc acttcccgaa tcatggttta tttcccggat ggcaacagca
     6661 gccaaccaca tgatcattca ccagcccaca tgcctgcata aaggagtaac agattgtggt
     6721 accgacaaac ttaaaaccac gtttttttag tgccttagat agggcgtcgg aggcgggtgt
     6781 agatgtgggg atttcgctca acgttgtggc ttgtgtcacc tgtggttgat gattgacaaa
     6841 cgaccagaca aagtcggcaa acggttcgcc gttctgttcc atttgcaggt acgcccgcgc
     6901 attaccaata attgcctgaa ttttcccgcg atggcggata atcccggcgt cctgtaccag
     6961 tctttcgaca tcctcttcct gcattgcagc caccttcacc ggatcgaact gatgaaagct
     7021 ggcgcgatag ttttcgcgtt ttttgaggac ggtgatccac gataatccag cctgctgccc
     7081 ttcaaggcag atcatttcga acagtttttt actgtcagtt tcaggcacgc cccactcatt
     7141 atcatggtag gcaatataaa gcgggtcctg actcacccag ccgcaacgtt ccatactttc
     7201 cctcgctatg tacccgatcg taaaaatttc agccgctttt cttgacgcat cggtgaaaaa
     7261 gtcaatattt aatacagggc tactatgccc gatatccttc atacaatgac aataatattg
     7321 ccgaattcgg ctcttccctg gagtcgtgtt aatgatcata aaaaaaagcg gtggtcgctg
     7381 gcagctaagc ctgctggcga gtgtggtaat cagtgccttt tttctcaaca cagcttacgc
     7441 ctggcaacaa gaatatatcg ttgatacgca acccggacat tccacagagc gttacacctg
     7501 ggatagcgat catcaacctg attacaacga tattttgtcg caacgtattc aaagtagcca
     7561 aagggcgctg ggactggaag tcaatctggc ggaagaaact cctgtggatg tgaccagcag
     7621 tatgagtatg ggctggaatt ttcctttata tgagcaggtt acaaccggcc cggtcgcggc
     7681 attacattac gatggcacaa ccacctcgat gtataacgag tttggcgaca gtactaccac
     7741 gctggccgat ccgttatggc atgccagcgt gagtacctta ggctggcgtg ttgactcccg
     7801 gcttggcgat ctccgaccct gggcgcaaat cagctataac cagcaatttg gcgagaatat
     7861 ctggaaggcg caatcaggcc tgagccggat gacggcgaca aaccagaacg gcaactggct
     7921 ggatgtcacc gtaggcgctg atatgttgct caatcaaaat attgctgcct atgctgcgct
     7981 atctcaggca gaaaatacca ctaacaatag cgactatctg tatacgatgg gggttagcgc
     8041 cagattttaa cgttatagat acaattgaaa ccaataaata acaactaatg tggcgatagt
     8101 gggtgataac ttactaaata ataaatttgg tgaataattg tcgagtcatt cattcctgaa
     8161 ctaatgcatt tcattccgtt ctgatggcat ttcatgccgt ttttccccag gcataaagtg
     8221 cacttcgtta tggttgtcag cagagatttt tcctttttat tactgcagga atactgccat
     8281 gacaccttca aattatcagc gtacccgctg gttgacactc atcggtacta tcattaccca
     8341 gtttgccctt gggtcggttt atacctggag cctgtttaac ggcgcgcttt ctgccaagct
     8401 ggatgcgccg gtaagccagg tcgctttctc tttcggcttg ttaagtctgg ggctggcaat
     8461 ttcatcttct gttgcgggga aattacagga acgttttggc gttaaacgcg tcactatggc
     8521 ttccggcatt ttgctgggat taggcttctt cctgacagcg cattctgaca acctgatgat
     8581 gctgtggtta agcgccggtg tgctggtcgg tctggcggat ggcgcgggtt acctgctgac
     8641 gctctctaac tgcgtgaagt ggttcccgga gcgtaaaggt ctgatctccg cgttcgctat
     8701 cggttcttat ggtctgggta gcctgggttt caaatttatc gacacgcagc tgctggaaac
     8761 ggtgggtctg gaaaaaacct ttgtgatttg gggagcgatt gcgctggtga tgattgtctt
     8821 tggcgcaaca ttaatgaaag acgcaccaaa acaggaagtg aaaaccagca atggtgtggt
     8881 ggagaaggac tacaccctgg cagagtcgat gcgtaaaccg cagtactgga tgttagcggt
     8941 tatgttcctg actgcgtgca tgagtggtct gtatgtgatt ggtgtagcga aagatatcgc
     9001 tcaaagtctg gcgcatcttg atgcaatttc cgcagccaat gctgtgacgg ttatttccat
     9061 cgccaacctt tctggtcgtc tggtgctggg cattctgtct gacaaaatcg cccgtatccg
     9121 tgttatcacc attggtcagg tgatatcgct ggtgggtatg gcggccctgc tgtttgcacc
     9181 attgaatgca gtgacgttct ttgcagcgat tgcctgcgtg gcatttaact ttggcggcac
     9241 tatcacggtg ttcccgtcac tggtcagtga gttcttcggc ctcaataacc tggcgaaaaa
     9301 ctacggtgtg atttatctcg gtttcggtat cggcagcatt tgtgggtcga ttatcgcctc
     9361 actgtttggc ggcttctatg tgaccttcta cgtgattttc gcactgctga ttctgtcact
     9421 ggcgctctct acgacgattc gtcagccaga gcagaaaatg ttgcgtgagg cgcatggctc
     9481 cctttaatca tctgccgcga gctactgggc tttagcctta gtgtgaatca aacgcttcaa
     9541 cgcaatgtat aaacaatgac gcgtaaaatt tgggccagtt aactggccct ttttactgct
     9601 ttttgtaacg ctaaagtctc ttttcaaact tgcatttttg taaatttgtg cttcatgcac
     9661 actctttccc cacacttttt ccctttgctg tggtctactt attcgcgcgt gtagatttta
     9721 cttatctgac tacctccgca cttttcccct gccgggcctg aaaagccgct aagcagggtg
     9781 ttatcacctg tttgtccagg gtttgtttgc atgagataca tcaaatcgat tacacagcag
     9841 aagctgagct ttttgcttgc aatctatatt ggccttttta tgaatggcgc ggttttttac
     9901 cgccgcttcg gcagctatgc gcacgatttt accgtctgga aaggcatttc tgctgttgtt
     9961 gaactggccg ccaccgtact ggtgaccttc tttttactac gtcttctttc gctgtttggc
    10021 cgccgtagct ggcgtattct ggcgtcgctg gtggtgctct tttccgcagg tgccagctat
    10081 tacatgacct tccttaatgt ggtcattggt tatggcatca tcgcttccgt catgaccacc
    10141 gatatcgacc tgtcaaaaga agttgttggt ctgaacttta ttctctggtt aatcgccgtt
    10201 agcgcgttgc ctcttatcct tatctggaat aaccgctgtc gttacacctt gctccgacaa
    10261 ctgcgaaccc cagggcagcg tattcgcagc ctggcggtcg tcgtactggc aggtattatg
    10321 gtttgggcac cgattcgttt gctggatatc cagcagaaga aagtggagag ggcgacaggt
    10381 gttgatttgc cgagttatgg cggcgtcgtc gcgaactctt atctgccatc aaactggctt
    10441 tcggcattag gtctttatgc ctgggcgcgg gtcgatgaat cttccgataa caattctctc
    10501 cttaaccctg cgaagaagtt tacgtaccag gctccacaaa acgttgatga tacatatgtc
    10561 gttttcatta ttggtgaaac cacgcgctgg gatcatatgg gcattttcgg ctatgaacgc
    10621 aataccacac cgaagctggc ccaggaaaaa aatctggcgg cgttccgtgg ttactcctgt
    10681 gataccgcaa ccaaactctc tctgcgttgc atgtttgtac gtcagggggg cgcggaagat
    10741 aatccgcagc gcacattaaa agaacagaac attttcgcgg tattgaagca gttaggattc
    10801 agttctgatc tctacgctat gcaaagcgaa atgtggttct acagcaatac gatggcggac
    10861 aacattgctt atcgtgagca gattggtgcg gagccacgta accgtggcaa accggtagat
    10921 gatatgttgc tggtagacga aatgcagcaa tcgctggggc gcaacccgga tggtaagcat
    10981 ctgatcattc tgcataccaa aggctcacac tttaactaca cccagcgtta cccgcgcagc
    11041 tttgcgcagt ggaagccgga atgtattggt gttgatagcg gctgtaccaa agcgcagatg
    11101 atcaactcat acgacaactc ggtgacctat gtggatcact ttatttccag cgtgattgat
    11161 caggttcgcg ataagaaagc gattgtgttc tatgcagctg accatggtga gtcaattaat
    11221 gaacgcgagc acctgcacgg cacgccgcgt gaactggcac cgccggaaca gttccgcgtg
    11281 ccgatgatgg tctggatgtc ggataaatat ctggaaaatc cggtcaatgc gcaggcattt
    11341 gcgcagctga aaaaagaagc tgacatgaaa gtgccacgcc gtcacgtaga gctgtacgac
    11401 accatcatgg gttgtcttgg ctatacttca ccggatggtg gaattaacga aaacaacaac
    11461 tggtgccaca tcccgcaggc aagagaggcg gcggctaact aagaggcctg ctgactttct
    11521 cgccgatcaa aaggcatttt gctattaagg gattgacgag ggcgtatctg cgcagtaaga
    11581 tgcgccccgc attcggtgat tggcgcagcc tggtagcgca cttcgttcgg gacgaagggg
    11641 tcggaggttc gaatcctcta tcaccgacca aattaaccat cggtaatcac cggtaagaca
    11701 cggaaagccc gctaatttaa tgattggtgg gcttttttgt attgtttagt caccagtaat
    11761 cacctgaaag ggatggttat aaccggattt tttagatata tgtttagcta tacggtctgc
    11821 catatagcta aatccgtata gctaaaaccg gataatcaca cacaacaggt gagtacatgg
    11881 cgagaaaaac cacccccctc acaaccacgc agatcaaagc cgcacgacca ggtgaaaagg
    11941 aactgacgct gcatgatggc ggcggactgt acctgttaat taaaccgtca ggggcgaaga
    12001 tatggcgatt cagttactac aggccagtag acaaaaagcg tgcgttaatg agcctgggat
    12061 cactggatga aatccccctg gcagaggcaa gaaggttacg aaacgaatac aggtcgttga
    12121 ttgcgtcagg aactgacccg caggaccatg aacggcgaaa acgtgaggct gaggcccgca
    12181 gacagggcaa cacgtttgaa aatgtggcgg cagcctggta tcagatgaaa atcagccaga
    12241 atctggcccc caacacgatt aaggacatct ggcggtcact ggataaatat gtattcccgt
    12301 tcatcggcaa taccccgata gacaccctca ccgctcacag gttcgtagaa atacttaccc
    12361 ccatcaaaga gcgcggcaat ctggaaacac ttaagcgggt tttgcagcgt gttaatgagg
    12421 taatggatta cgccgccaac agcgggctga ttgacagcaa tccggctatg aatgtgcgta
    12481 aagcgtttcc ctcccctgta aaaaaacata tgccgacaat caggcctgag cagttaccgg
    12541 agcttatgct tgccgttgct ggtgcatcca tagaacggca gacaagacta ctgattgaat
    12601 ggcagttgtt gacagtaaca cgccccgccg aagcatcatc ggcacggtgg gaggaaatcg
    12661 accttaagtc cagtacatgg acgatccccg ccggacgtat gaagatgcgc cgcgaccacg
    12721 ttatcccgct ttgtgatcag gctatggcag tacttgaagc catgaagcca atcagccatc
    12781 accgcgagta tgtttttcca ggcctgaaag acccgcgcca gccaatgaac agtcagaccg
    12841 ccaatgttgc attacgccgt atgggattcg gcgggattct ggtttcccac ggtatgcgcg
    12901 ccatattcag cacggcagcg aacgaggaag gatttgagcc ggacgtaata gaagcggccc
    12961 ttgctcatgt ggatatgaac agcgtgagac gggcttacaa caggagtaac taccttgaaa
    13021 aacgcattgt gctgatgcgc tggtggggtg agtttgttga ggcagcggca acaggtgtga
    13081 cgcttgccag cggtaaaagg ggcattcggg cggtacagaa ctgaacggac tgttcaataa
    13141 ctacactgat tgccaaagaa ctgtacgaac aaacagtaaa aacggcgggt tccggcgcat
    13201 aacgtggtat aatggcggca aatttaacga caaggccgag aggtatgagt ccgttaatat
    13261 gcaagacaca caacacaact ctgaaggtat gcaactggcg ctactggtta accgtgagtt
    13321 ctggtcaacc tacgaccccg aagacaaaag cacggcccct tccagcaggg aggtaataga
    13381 ttttctggta tcccgtggtg cttccaaaaa tctggcggta agtattgata aggtcgtacg
    13441 tccggcatcc atgaaagctg gtggcaggcc aagaaaatgg cggtaacact ccctgaggcg
    13501 gcagaaatgc cgcttttttt atggtgataa aaattaagtt atagaaaaac tattcaccac
    13561 tgttcaccct gcaataaatg tatttagtat caatatgtta atgggtgaac agttttgtaa
    13621 aactattcac caaagtgttc acctgttcac cccagaacga taaaagggtg ggtaccgttt
    13681 aaaaacggtg ggtactgttt tttctggttt agccgtttta ccgtatttct caccggaata
    13741 agccggataa taaggagtac acgatggaaa caatcagaaa aatcattgtt cgcacagaag
    13801 taaaaaaact catcaatatc aaatcagaca gcacgctaca ggccatgatt aaggctgggg
    13861 aatttccggc gggttttcgt attggtctgc gtcgtcgcgg atggtatgag gatgaagtgc
    13921 tggcctggct gaaggaacgc gaacaggaag cgcgcggtgc agttgcataa agggggataa
    13981 aaagggcatt cagcaactga ataccctgag tgacaggaga aaactcaatg gaagcaatca
    14041 tgctaccgcc acgataaaga cactgcaatc ttgctggagt aaatcaggaa cacattcagg
    14101 agtttttcaa tgaaacgtcc gaatatgccc gtacaggggc agggcttcgt tcagcctgaa
    14161 atcagccaga acgatatatt gattcgccac aaagcggagt cgcgtattga ttcccgcgtt
    14221 atttctgagc gagcaggaat acaacacgaa agtatcattg cgacgattga atctcatcaa
    14281 tccagattgc gcgagcttgg ctcactgcct cgccagtcag tcagtcaagg aattaaccta
    14341 tttgaaatcg ggaaaacctg gccgcaagcg cggtcgtcct gaaattagtt atttgctgaa
    14401 tgagctacag cttgactatc tgttacgcgt tatccgtggg cgtgacgcag aacgtatcaa
    14461 ccagttcaag ctggatgtta caaaggcatt cgcgaagcat cgcgccgctg aaccagtccg
    14521 ccgcgaatat ctgccaggct atcacgaaag ccgtgacgga ctcaaagcac tgggcgcaga
    14581 acgtcaccat tacattaatc tggcacgcgc agagaaccgt gttacaggtc tggtggctgg
    14641 agaacgcagc acagccagcg agcaacagtt aggcttactg atagtcatgc agaaaatcga
    14701 gcaggcagca tttgacgagg cgatacagag cggcatgaca ccgaccgaag cggtgcggga
    14761 ggttgcccgc cgcatggaga cgttcgccag cctgatgacg tcagcgtcgt ctctgggggt
    14821 gaaacatgct taagaatttt gccgaattaa caccggaacg agtggagttt atgcgccata
    14881 tcgtgagcca tttcgccgct gagcgcatga aacacgttaa gcaatacctg gaggaggttt
    14941 tatcaaatta tcgcgctggt ggccctgtac ggcgcgaata tttgcatgat gcaggaatgg
    15001 caggcaaaac aaaggcgacc acggaagggt cgccagtgga gagaaaggaa atcaagcgca
    15061 ttatccacaa tgccatatct ga
//
LOCUS       DAZKGF010000122        14884 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZKGF010000122 DAZKGF010000000
VERSION     DAZKGF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.122"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(406..1407)
                     /locus_tag="ACHOQV_003539"
     CDS             complement(406..1407)
                     /locus_tag="ACHOQV_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9631846.1"
                     /translation="MSSWLAMLQDATEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGKGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIEKPVVNCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSEWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRIPMIVAFLTVILGMAILMGYLTQMLF"
     gene            1514..1810
                     /locus_tag="ACHOQV_003540"
     CDS             1514..1810
                     /locus_tag="ACHOQV_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9631847.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1933..2358)
                     /gene="arsC"
                     /locus_tag="ACHOQV_003541"
     CDS             complement(1933..2358)
                     /gene="arsC"
                     /locus_tag="ACHOQV_003541"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFP9631848.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIVYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQQGAFTKEDGEKVVDEAGKRLK"
     gene            complement(2371..3660)
                     /gene="arsB"
                     /locus_tag="ACHOQV_003542"
     CDS             complement(2371..3660)
                     /gene="arsB"
                     /locus_tag="ACHOQV_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFP9631849.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVILHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLFLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTSKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLTALALRLSFTL"
     gene            complement(3715..4068)
                     /gene="arsR"
                     /locus_tag="ACHOQV_003543"
     CDS             complement(3715..4068)
                     /gene="arsR"
                     /locus_tag="ACHOQV_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFP9631850.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLSLLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQVIVRN
                     LARQNCSADSKNICS"
     gene            complement(4316..4531)
                     /locus_tag="ACHOQV_003544"
     CDS             complement(4316..4531)
                     /locus_tag="ACHOQV_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631851.1"
                     /translation="MMAISLQSEPPTQGWKVKNRRKVGFLHPDSDKAWYGSDPMKVYK
                     SSFTNMYSKLSLRQLKKSLTLAMLATG"
     gene            4810..4893
                     /gene="dinQ"
                     /locus_tag="ACHOQV_003545"
     CDS             4810..4893
                     /gene="dinQ"
                     /locus_tag="ACHOQV_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFP9631852.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4947..6299)
                     /gene="gorA"
                     /locus_tag="ACHOQV_003546"
     CDS             complement(4947..6299)
                     /gene="gorA"
                     /locus_tag="ACHOQV_003546"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP9631853.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6371..7213)
                     /gene="rlmJ"
                     /locus_tag="ACHOQV_003547"
     CDS             complement(6371..7213)
                     /gene="rlmJ"
                     /locus_tag="ACHOQV_003547"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFP9631854.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLSSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATASW
                     IVPE"
     gene            7416..9458
                     /gene="prlC"
                     /locus_tag="ACHOQV_003548"
     CDS             7416..9458
                     /gene="prlC"
                     /locus_tag="ACHOQV_003548"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFP9631855.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILIRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            9466..10218
                     /gene="rsmJ"
                     /locus_tag="ACHOQV_003549"
     CDS             9466..10218
                     /gene="rsmJ"
                     /locus_tag="ACHOQV_003549"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFP9631856.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10267..11736)
                     /gene="dtpB"
                     /locus_tag="ACHOQV_003550"
     CDS             complement(10267..11736)
                     /gene="dtpB"
                     /locus_tag="ACHOQV_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFP9631857.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(12051..12485)
                     /gene="uspA"
                     /locus_tag="ACHOQV_003551"
     CDS             complement(12051..12485)
                     /gene="uspA"
                     /locus_tag="ACHOQV_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFP9631858.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12876..13211
                     /gene="uspB"
                     /locus_tag="ACHOQV_003552"
     CDS             12876..13211
                     /gene="uspB"
                     /locus_tag="ACHOQV_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFP9631859.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13282..14781)
                     /gene="pitA"
                     /locus_tag="ACHOQV_003553"
     CDS             complement(13282..14781)
                     /gene="pitA"
                     /locus_tag="ACHOQV_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFP9631860.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
BASE COUNT         3832 a         3787 c         3847 g         3418 t
ORIGIN      
        1 tgatagtatc gtaaaggaaa acgttaacaa ctatcaaaaa gcgctcattt taaaaagtta
       61 tgatgttcac cgatagtgtg tgaagtgata tttttgtgat gttttagatg tgtaataatg
      121 aaccaggtta aagtgtattt aaaaatgtgt tgtattggcg actgaaatct gccctttaaa
      181 aggggggata gcactgaaac aaagagacat tgcttctccg ataaaatgtc agagaagcat
      241 gattgcgtgg tgattgtcga ttgataactt actgacggat ccgtcatcat tctcaaagca
      301 ttagttaagt tatttgtgat ttttaaggat tatcctatct atgattttct actcagaagg
      361 ataaggcact cacaatgctc cttatagttt actgctggca tctatttaaa atagcatttg
      421 agtcaaatag cccatcaaga tagccatacc taatataacc gtcaggaatg caactatcat
      481 cggtattctg aacattgatt taagtaatat cacttccgtc aggcttgcgc cggcactgcc
      541 gatgattaat gccattaatg ctcccagacc cattcctttt gtcatcagaa cagatgccag
      601 agggataacc gcctctgccc ggatatatag cggaataccg acaacggcgc tcaatgggat
      661 ggcgaaggga ttatctgccc ctgcatgagc ggcaatccac tctgagggaa taaagccata
      721 aataaaagag cctattaata cccctaacaa aagatatggc agtacatctt taaactgttg
      781 caacgcatct ttggtggcta gttttatggg attaatactt accacagctt tggtactgca
      841 acagtttact acaggtttct caatggcttt agccgctgga ctacagcaac tcacttttat
      901 tggcgtatat gtcgtccccg gcgaagtttt ggctggagca caacaatttg ctgatgagct
      961 tttactggca atgatatgac gttcaaatcc cagagaatcg aggataatac tggcaagtac
     1021 ggagacgccg gcggcgataa tcgcatacaa caaggtaact ttccagccaa aggtcaccca
     1081 cattaacccg acgataatgg gattaagtaa tggggaaaca aataaaaaag tgagggtcgg
     1141 accaaacccg gctttcgctg ataacaatcc acgtaacata gggattgtcg agcaactaca
     1201 gaacggggtg acggctccca acagagcagc caggagatag cctttccctt ttcttgcccc
     1261 catcatctgc tggattttat ggtctggcac cttttgtctt atcaggctca caccggcact
     1321 aatcactata aacagcaaag aaagctcgac ggcgagaaac acaaacatct ctgtggcatc
     1381 ttgcagcatc gcaagccaac tactcataca taacctcatt gaataaatct atatttccag
     1441 aataatcgaa atgaaattca gatcaagata tttctggtat tatcgaaata tcaaccagac
     1501 agagggggat catatgcagt tggaagaagt cgctaaagcg ctgaaagagc taggtcaccc
     1561 gacccgtttg ttcatattta aacatcttgt caaagccggg gaacaagggt tgccggtcgg
     1621 tgagttgcaa aagcagcttg ggatacccgg ctctacactc agtcaccata tttcagcact
     1681 ggtatcggta gggctggtca aacaaaaccg tgagagtcga acgctgatgt gtgtttcgca
     1741 atacgcaatg ctggaagcga taattgaatt cttacgcgag gagtgctgcg taaatagcaa
     1801 aattgcctga tgcgctacgc gctacagatt tcatgccatg cacggttttg taggtcggat
     1861 aagacgcgtt agcgtcgcat ccgacataaa caaagcgcac tttaccaact atgggcagtg
     1921 gatatcgccg ctttatttca agcgtttacc cgcttcatca accactttct cgccatcttc
     1981 cttggtgaac gcgccttgtt gcgcatctgg cagaatttcc agcacgactt ctgaagggcg
     2041 gcacaggcga gttcccaacg gcgtcaccac aatcgggcga ttaatcagaa tcgggtgctg
     2101 aagcataaag tcgattaacc gatcgtcagt aaatttatct tctgcaaggc ccagttcctc
     2161 atacggctcg acgtttttac gcagcagcgc gcgtacggaa atccccatat cggcaatgag
     2221 tttgaccagt tcatcacgcg tcggtggggt ttccagataa tagacaatgg tcggttctgt
     2281 gccgctgttg cggatcatct ccagcgtgtt acgcgacgtg ccgcaggccg ggttgtgata
     2341 aatggtaatg ttgctcatat caatatctca ttacaaagtg aaagagagac gtagcgccag
     2401 cgcagtcagc gtcacaaaca gcacaggcag ggtcatgata atccctgtac ggaagtaata
     2461 tccccagctg atagtcatat tcttctgcga aagtacgtgc agccagagca gcgtcgccag
     2521 gctaccaatc ggggtaattt tcggccccaa atcgcagcca attacgttgg cataaaccat
     2581 cgcttctttg ataacgccag atgccgtgct gccatcaatg gacaacgcgc caaccagtac
     2641 cgtcggcata ttgttcataa tagaagagag gaaggcggtg aggaatccgg tgccgagcgt
     2701 cgcggcccac aggccattat ccgccagcac gttgagtacg ccagaaagat attccgttag
     2761 tccggcattg cgcaggccat aaaccaccag atacatgccg agcgagaaga tgacaatctg
     2821 ccagggcgca ccgcgtagca ccttactcgt attaatcgca tgaccgcgtt tagcgacgac
     2881 aaataatatc aacgcgccca ctgccgcaat ggcgctgacc ggaatgccga gcggttccag
     2941 gacgaaaaat cccaccagca gaaacagtaa aacaacccag ccagttttga aggtggcagg
     3001 atctttaatc gcttctgcgg gagatttcag cagcgccata tcgtagttct gcggaatatc
     3061 tttgcggaaa tagagatgca aaatcaccag cgtggcaaca attgcggcga tatccaccgg
     3121 caccatcacc gatgcgtatt cgcgaaagcc gaggccaaag aaatctgcgg aaacgatatt
     3181 caccaggttg gagacaataa gcggcaggct ggcggtatcg gcaatgaatc cggccgccat
     3241 cacgaacgcc agcgtagtgc ctttactgaa ccctaaagcc agcaacatgg cgatgacaat
     3301 cggtgtcaaa ataagcgccg cgccatcatt ggcaaacaga gctgcaacgg cagcaccgag
     3361 caggacaatc caggtgaaca gcaagcgacc gcgtccattg ccccagcgtg agacgtgcag
     3421 cgccgcccat tcaaaaaagc cggactcatc cagcagcagg ctgatgataa tgacggcaat
     3481 aaacgccgcc gtcgcgttcc agacgatatt ccacaccacc ggaatatcac ccggatggac
     3541 cacgcccgta actaacgcca gtactgcgcc gagcgttgca ctccagccga tgcctaaacc
     3601 tttcggctgc cagataacca atacaatggt caggacaaag atagcgcctg ccagtaacat
     3661 aatgcctccc ggataaaaca catgcgaaaa tacatatgtg tttagctaaa ttttttaact
     3721 gcaaatgttc ttactgtctg cggaacaatt ttgtcgagcc aggttgcgga caatcacctg
     3781 aaccttttcc tgttcacatc gccaggcctc atcaataatt ttcgccgccc atgctggaat
     3841 atgcggtgat aagcggtaat gaacccactt accttgcttg cgatccagca atagcccact
     3901 ttcacgcagc aatgacagat ggcgggatat cttgggctgc gactgatcga gggcagtgca
     3961 gagatcgcag acgcataact ctcccagttc gctaagcagt aaaacgatgc ccagacgggt
     4021 ctcatcagca agaattttga acagttgaat gggtaacaga aatgacatat tgcgctcctg
     4081 attgttgcag gtagtgtctc tcttcgaagc ggataagtca aaaacatata tggtttaacg
     4141 aatgtgtaag tgcagaggaa ggtaataggt gtgaattttg agttgggtat tcatttgaaa
     4201 ggaggtctga atcaggacgt aaaaaaaccg actttgcgtc ggttttttac tttccagccc
     4261 tgagttggtg gctctggctg gagtgagaat gcccacatct aacggttcaa tactattagc
     4321 cagtggctaa cattgcaagc gttagcgatt tcttcagctg gcggagggac agtttgctat
     4381 acatgttggt gaaagaggat ttatatactt tcatcgggtc gctgccgtac caggccttgt
     4441 ccgaatccgg atgtaaaaaa ccgactttgc gtcggttttt tactttccag ccctgagttg
     4501 gtggctctga ctggagtgag atagccatca tctaacgcat caatgctaat accatactga
     4561 aactattgca aggacatgct ggttttataa cccgcaagta ctgtatgatt atccagttag
     4621 ctctgagaca ttttcactct ggcaatgcgc ataaacgctt tcaaagtcct ggtcagaagt
     4681 acgggtggtg ccgttaactg atgctctggc cggagtgaga atgcccacat ctaacaatgg
     4741 ggcatgcgcc gtgacaggca gtggatgagt aagcggatgc attctcactc catcgcatgg
     4801 agaaaacggg tgattgataa agcaatcatc gttcttgggg cgttaattgc gctgctggaa
     4861 ctgatccgct ttctgcttca gcttctgaac tgatagcgga aacgtagcta agggctaaga
     4921 gcacactact cttagccctt taacatttaa cgcattgtca cgaactcttc tgccgctgtc
     4981 gggtgaatgg caaccgtgtt gtcgaagtct tttttggttg cgcccatctt cagcgccacc
     5041 gcgaagccct gcaacatttc atccatacca aaaccaatgc cgtgaattcc gacaatcttc
     5101 tcttccggtc caacgcatac cagtttcatg cggcacggct ggcggtgagt ggtgacggcg
     5161 gtatacatcg cggtgaaaga ggatttatac actttcacct gatcgtcgcc gtactgctcg
     5221 cgcgcctgcg gttccgttaa accaacagtc ccaatcggcg gatggctgaa gaccacggtc
     5281 ggaatgttgc tgtaatccag atgctcatcc ggcttgttat taaacaggcg ttcagagaga
     5341 cggcgacccg ctgcaactgc caccggtgtc agttccactg cacccgtgtt atcgcccacc
     5401 gcgtaaatac cttcaacgtt ggtattttga tatttatcga cgacgatata gcctttttcg
     5461 ttagttttaa cgccagcggc ttccaggttg atgttgtcat tggcaggctc gcgaccaatc
     5521 gcccaaatca ggcaatccac cgtttcactg cgcccatctt ccagctccag cgtcaggcta
     5581 ccatcagcat ttttcactac tgctttcggg atggcgttgg tatgcaactg cgggccttcg
     5641 gcgttcatca cttcgaccag cgtttcagag atcatcgggt cgaagctgcg cagcggcgca
     5701 tgtttacgca caaacagatg cgttttcgcg ccgaggccgt taatcacgcc cgccagttcc
     5761 acggcgatgt aacctgcgcc aacaaccgcc acgcgctctg gcaaagcagg aagggcgaag
     5821 aagccatcag aatcaatacc gtattccacg cctggaatat ccgggtggct cggacgaccg
     5881 ccggtggcga tcagaatatg atcggccgtg atggtttcgc cgtttacctc cagcgttttg
     5941 gcatcaacga agcgggcaaa gcctttgatt acatcaacgt tatttttacc gagcacgttt
     6001 tcataggaag tatgaatacg gtcgatatag gcggtacggc tggcgatcaa cgtttcccag
     6061 ttgaatttat tgatagtggt atcgaaacca taatccggac cgtacatgtg gatcgcttca
     6121 cggatttgcg ccgcgtgcca catcactttt ttcggcacgc agccgacgtt cacgcaggtg
     6181 ccgcccagtt ctttggcttc aatcagcgcg catttctgac cgtacatagc cgcgcggttg
     6241 atggaggcga taccgccgct gccgccgccg atggcgatgt aatcatagtg tttagtcatg
     6301 acatattgtc cttatcgttg attaccgcga ttgtagcgcg agacgtaata ggtgccagcg
     6361 atggctgcaa ttactccggc acgatccagc ttgcggtggc gtgcccagtg ccagctggaa
     6421 ccagtttgct gtgcagccac ggcagcacgt tattcatctg ctgttccagc ttccacggcg
     6481 gattaatcac aatcatgccg gaagcggtca tgccacggcg atcgctgtct ggcagcaccg
     6541 ccagttcaat ttgcagaatt ttgcgaatgc cggtcgcttc caggtcgtgg atcatgcgct
     6601 taatttgctg gcgcagcacc actggatacc acaatgcgta agtgccagtg gcgaaacgtt
     6661 tgtaaccttc tgctatcccg ctgaccaccg cctgatagtc ggttttcatt tcatacggcg
     6721 ggtcgataag gattaaacca cggcgggaaa ccggcggcag cttggcctta agctgctgga
     6781 aaccgtcggc tttttcgacg cgcgcacggc tgtctttctg aaattcagaa cgcagcaacg
     6841 ggtaatcgct cgggtgcagc tcggtcagtt gcaggctgtc ctgttcacgc agtagctggc
     6901 gagcaatcaa cggcgaaccg gggtaataac gcaactgccc gctacggttg aagtgtttta
     6961 ccacattgat gtacgcctcc agctccgcag gcaaatcgtc ctgctgccag atacgggcga
     7021 taccttcgag atattcgccg gtacgctcgg catgttcgct gcttaactga taacgcccgg
     7081 cccctgcgtg ggtgtcgaga tagagaaacg gtttatcttt ctctttcagc gactcgatga
     7141 tcaggctctg aacggtatgt ttaaggacgt cggcgtggtt gccagcgtga aagctgtggc
     7201 gataactgag catgggtaaa ggtgttccgg taagtaaaaa tcggcccgta ttcggggcgc
     7261 acaaaagcct atcaggacag tataccgaaa agaggccgcc gccgcgaaag cgtaacgttt
     7321 ctcattgaaa ttcactacac ttaaccccat gctacacaca ttatgtaaag cgcctgttga
     7381 gcgcttcctt aacctcttta accaggactg cgctaatgac gaatccgtta ctgactccct
     7441 ttgaattgcc tccgttttct aaaattctcc cggaacatgt cgttccagcc gtgactaagg
     7501 cgctgaatga ctgccgcgaa aacgtggagc gcgtagtagc gcaaggggca ccgtacacct
     7561 gggaaaatct ctgccagccg ttggcagaag tagacgatgt gctggggcgt atcttctccc
     7621 cggtcagcca cctgaactcg gtgaaaaata gcccggaact gcgtgaagcg tacgaacaaa
     7681 ccctgccgct gctgtcagaa tacagcacct gggtagggca acatgaaggg ctgtataaag
     7741 cgtatcgcga cctgcgcgat ggcgatcatt acgccacgct gaacacggcg cagaaaaaag
     7801 cggttgataa cgcactgcgc gacttcgaac tctctggcat cggtctgcca aaagagaaac
     7861 aacaacgcta cggtgagatt gccacccgtc tttccgagct gggcaaccag tacagcaaca
     7921 acgtcctcga tgcgacaatg ggctggacca aactcgttac cgacgaagcg gaactggcag
     7981 gaatgccaga aagcgcactg gctgcggcaa aagcccaggc tgaagcgaaa gagctggaag
     8041 gttatttgct gacgctggat atcccaagct acttgccggt aatgacttac tgcgacaacc
     8101 aggccttgcg tgaagagatg tatcgtgctt acagcacccg cgcttccgat caaggcccga
     8161 acgccggtaa gtgggacaac agcaaggtga tggaagagat cctcgcgctg cgtcacgaac
     8221 tggcgcaact gctgggtttt gaaaactacg cctttaaatc ccttgccact aaaatggcag
     8281 aaaatccgca gcaggtgctt gatttcttaa ccgatctggc aaaacgcgcg cgtccgcaag
     8341 gtgaaaaaga gctggcgcaa ctgcgtgcct tcgctaaagc cgaatttggc gtcgatgagt
     8401 tgcagccgtg ggatatcgct tactacagcg aaaaacaaaa acagcatctc tacagcatca
     8461 gtgacgaaca gctgcgtccg tacttcccgg aaaacaaagc ggttaacggc ttgtttgaag
     8521 tggtgaaacg tatttacggc atcaccgcta aagagcgtaa agatgttgat gtctggcatc
     8581 cggatgtacg tttcttcgaa ctgtatgacg agaacaacga actgcgcggc agcttctacc
     8641 tcgacctgta tgcccgtgaa aacaagcgcg gcggggcgtg gatggatgac tgcgtaggcc
     8701 agatgcgtaa agccgacggt tcgctgcaaa aaccggtcgc gtatctgacc tgtaacttca
     8761 accgcccggt aaatggtaaa ccggcactgt ttacccatga cgaagtgatc accctgttcc
     8821 atgaattcgg tcacggcctg caccatatgc tgacccgcat tgaaactgct ggtgtatctg
     8881 gtatcagcgg ggtgccgtgg gatgcggtcg aactgccgag tcagtttatg gaaaactggt
     8941 gctgggagcc ggaggcgctg gcgtttatct ccggtcacta tgaaaccggc gaaccgctgc
     9001 cgaaggagct gctggataaa atgctggcgg ctaagaacta ccaggcggcg ctgtttattc
     9061 tgcgccaact ggagttcggc ctgtttgatt tccgcctcca tgccgagttc cgcccggatc
     9121 agggagcgaa aatcctcgaa actctggcag aaatcaagaa actggttgcc gtagtaccgt
     9181 ctccatcctg gggccgtttc ccgcacgctt tcagccatat tttcgccggt ggttatgccg
     9241 caggttacta cagctacctg tgggccgacg tgctggcggc agatgccttc tcgcgttttg
     9301 aggaagaggg cattttcaac cgtgaaactg ggcagtcgtt cctcgacaac attctgatcc
     9361 gtggcggttc agaagagccg atggagctgt tcaaacgctt ccgtggtcgt gaaccacagc
     9421 tggatgcgat gctggagcat tacggcatta agggctgaac attcagtgaa aatctgctta
     9481 attgatgaaa caggcgccgg agacggcgcc ttatctgttc tggcggcccg ctgggggctg
     9541 gagcacgatg aagacaacct gatggcgctg gtgttaacac cggagcacct ggaactgcgc
     9601 aagcgtgatg agccgaagct gggcggcatc tttgttgatt ttgttggcgg ggcgatggcg
     9661 caccgacgca aattcggcgg tggtcgcgga gaggcggtgg cgaaagcggt gggcattaaa
     9721 ggcgattatt tgccggatgt ggtggacgcc accgcagggc tggggcgcga tgcctttgtg
     9781 ctggcttcgg tcggctgccg cgtgcggatg ctggagcgta atccggtggt tgctgcattg
     9841 ctcgacgacg gcctggcgcg tggttatgcg gatgcggaaa tcggcggctg gttgcaggag
     9901 cggttgcagt taattcacgc ttccagcctg acggcgctga ccgatattac tccgcgcccg
     9961 caggtggtct atctcgatcc gatgttcccg cataagcaga aaagcgcgtt ggtgaaaaaa
    10021 gagatgcgtg tctttcagtc gctggtgggg ccggatcttg atgccgatgg attgctggag
    10081 cctgcacgct tgctggcgac caaacgcgtg gtggtaaagc gcccggacta cgcgccgccg
    10141 ctggcgaatg tcgccacgcc aaacgcggta gtgaccaaag ggcatcgctt tgatatttat
    10201 gccggaacgc cggtgtaaaa gtaaagcagg gtagcgttat cgctaccctg ctttctgcaa
    10261 gaataattaa tggctttcgg gcgtcgcaat catgcgtttc agccacggca ccatcagcag
    10321 catcaccacc gcaacgccca gcgtaaccag gccaatctta ccaaacacgt tggtatagac
    10381 gggcaacgtc tcaagcggat cggtaatgtt gtctggtact gcggtaaatg ttgccacata
    10441 gccgcccagc aagaacgcgg cagcctgcgt caggaaccac atcccgagaa taaagcccat
    10501 caaatgctgc ggcaccaggg cagcaatcat cgccagaccc agggcgctaa taaacaattc
    10561 acccaggctc tggaacaagt aaaccagcac gataaaccac ggcgatgtca gtccttgtgc
    10621 atcagcaaac cacattcccg cagcggcggc tgtaagaaag cccagcgagc acataaacat
    10681 gccgagagta aatttcatcg gcatcgagag gtctttgcct ttgttaccca gatgcgtgta
    10741 aatgcctgcc agtattgggc tggcgagtac cacccagaac gggttaagcg cctggaagct
    10801 gactgggttg atggaaaagc cgagaatttc atgatgcacg ttgttgatag caaagaagtt
    10861 cagcgatgtt ggcatctggg cgtagagaat gtaaaacacc accgcttcga gcatcaggac
    10921 aaaggcgaca aacattttat tgcgcccggt tttatccagc ttgaatgcct gacgaaagaa
    10981 aatgatggtg acgacgatgg agagaacaat cagcaccaga ttggcgactt ctacgttgtg
    11041 catcagccat gcacagacga agatcatcac cacgctgcca agtaacacgt acaacagttt
    11101 gctgaagctc atcggccgga agtcgggttc agaaccaatg tctttcacca ttccgcgaca
    11161 ggcgatgtaa accaatagcg cgataattaa tcccgcaccg cacaggttgt aggtgactga
    11221 atagccgaat ctatcagcga tcacaggggc cagcgataac gctatcaacg agccgatgtt
    11281 gatcgacata tagaacaggg taaatgcgcc atcaagccgc ggatctttcg gcggatagca
    11341 cttcgaaagc aagctggctg ggttagcttt aaacaggccg ttaccgacag cgatagtccc
    11401 cagggcgatg aaaatcaggt caggcttaag tagcgacagg ccggtcatga agtagccaat
    11461 cgccagcaca agtgctccga ggacaatggt gcgtttggtc cccagcaggt ggtcgccgac
    11521 atagccgcca atggaaatga ggccatagac cagcgcagca aaagcaccaa aagtgacaaa
    11581 agcctgttct tgcgagaatc caagctgctt aacgaagaaa accgccagta cgccctgcac
    11641 gccgtagtag ccgaatcgct cccataactc gacaaaaaag atcatgaaaa atgggcgagg
    11701 ttgctgcagc atccccatgg gtgttgttgt attcatattt attaaccttc caataccatc
    11761 ctgaaaagta tgaacgcgta agggataacg cgcgaaaaag tttacatctg tcagagattc
    11821 ttaatattga tgcggtgaat agattaatac actattacaa cagaaaataa ccagctgatc
    11881 acactaatga tggaaaatat gatggtgtta aatgttgtat tggcagggga ttttgattgc
    11941 gtacgcagac cgtggccaga taaggtgttt acgctgcaag gtaacaaggc gcgccaaaaa
    12001 gcaaaacgcc cgcatatgcg ggcgttcagg aacacgtcgt agagggaaga ttattcttct
    12061 tcgtcgcgca gcggaacaat cagcatatca acgtgaacgg tgttgatcag ctgacgtgcg
    12121 gaagacatca gtttgctcca gaagtcctgg tggtgaccac aaaccaccaa atccatatcg
    12181 tatttcttga ttgcatcgac cagaacctgg cccaggtcgc cgctgccgct cagggtttca
    12241 gtgattgggt agcctgcatt agtggaaagc tcggtcagcg catgatgtgt ctcttcagag
    12301 atgcgtttct gcatatcacc cagattcaca tcaataagcc cggtgtatag gtcagagtag
    12361 tttacatcta cgtggatcag agaaactttc gcattgtagg ggcgagccat agagactgct
    12421 ttctctacca gaactttgct ttccggggag agatcgaccg cgatgagaat gtgtttataa
    12481 gccatagtgt tactccttcc ataaagttgt cgatgactgg ccagctagcg tttcttgtgc
    12541 cgttacgtca cccgcgtcct gcgaactacg tgcgcggggc taacccagcc gtaaagattc
    12601 agtgttaaga ccatccttac cttatagcga cccggatgat ccgtcaatcc gccttgctta
    12661 cccgttagat aaacaaattt tcgacatgaa tatattgata gtggttaacc ttctggaaaa
    12721 aaaacaacct gatctcctac actatctata gagccgctcg gatgttggtc accacaaaag
    12781 cggtttctcc aggacctggt gttaactgat tggcagcgta tcgggagcgg tagccccggg
    12841 gtggaactcc gcgggcaggt cgccggggag gaggtatgat aagcaccgtc gcattatttt
    12901 gggctttatg tgtcgtttgc attgttaaca tggcgcgcta tttctcatca ctacgcgcgt
    12961 tgttagtggt actgcgtaac tgcgatccat tgctctatca atatgttgat ggagggggct
    13021 tttttacctc acatggccaa cccaacaaac aggtgcgtct cgtttggtat atctatgccc
    13081 aacgttatcg cgatcatcac gatgatgagt ttattcgccg ctgtgagcgg gtgcgtcggc
    13141 agtttattct gaccagcgca ttgtgtggtc tggtggtggt cagtctgatt gcattgatga
    13201 tttggcatta agcacttcag caaaaataac gcataaaaaa agcgggccag taaactgacc
    13261 cgctcgtttt ggtgcgtgcg attacaggaa ctgcaaggag agccagtaca gcccgccgga
    13321 aagcagtacc gcagccggaa gggtaaacac ccaggccatc agaatactgg tcacggtttt
    13381 acgctgtaag ccgccgccat ctaccaccat cgtccccgcg acagaagagg agagtacgtg
    13441 ggtagtggaa accggcatcc cggtataact cgccaggccg atagacactg ccgcagtcat
    13501 ctgggcagac attccctgag cgtaggtcat gcctttctta ccgattttct caccgatagt
    13561 cgttgccaca cgacgccagc caatcatcgt accgatacct aacgccagcg cgaccgccat
    13621 gatgatccac accggtgcat actcgatggt gctaagcatg tcagacttca gtttcttcaa
    13681 caggcgctga tcgtcagcac tcacgccagg catcttcacc actttgtcga tagtgtcaga
    13741 aacgcacagc ataatgcggc gcatctggct acgttgatca agcgacagct tatcgtagct
    13801 ttccacatcg gtagtcaaca tgcctttcag gcggttgagc gcgttaatgg tattcgacgg
    13861 atggcaatgg aactccgcag gttgcgttgc gccagcttcc ggagccggta ctaactgatc
    13921 agcaccggtc gcttgtttca gcagcgcagg atgctgctca aagtaagctt cgacgttgtt
    13981 gatggcatca cgggtacggg tgatttcgta gccagtggca ttcatgttca ccacgaagcc
    14041 tgctggtgcg acaccaatca ataccaacat aaccagacca atgcctttct gaccatcgtt
    14101 cgcgccatgc gaaaacgcca cgccgatagc ggaaaggatc agcgcaatac gcgtccagaa
    14161 cggcggcttt ttcttgccgt ctttcttttc acgctccgct ggggtcaggt ggatacgggc
    14221 gcgtttcttg gtgccgctcc agtagcgacg cagcaagaaa atcagaccgc cagcaaacac
    14281 caggccgaca ataggggaaa cgatcagaga accgaaaata cttaatactt tcgggatatt
    14341 gagtgcatcc accactgacg tcccggtcat caacgcattg gttaaaccaa tcccgatgat
    14401 cgcgccaatc agcgtatgag agctggatgc aggtaaacca aagtaccagg tacccaggtt
    14461 ccagataatc gccgccagca acatagagaa caccatggca aggccatgag acgatcccat
    14521 attaagcagc agatccgtcg gcagcatatg cacaatggca taggcaacac tcagaccacc
    14581 cagcaaaaca cccaaaaagt tgaacaccgc cgccataacc acggcgagct gagaacgcat
    14641 cgcgcgggta tagataacgg ttgccacggc gttggctgtg tcatggaaac cattgatggc
    14701 ttcgtagaac agcacaaaag ccagtgcaag caataataac agcccggtat gcaaatccag
    14761 gccagcaaac aaatgtagca taggacgtta cgccattttg aggacatgaa cgcggcgcat
    14821 tatcagtgac tttcgcggcg cgggcaaagt gaaatataga ctttttttga tttgcctcct
    14881 gtat
//
LOCUS       DAZKGF010000123        14726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZKGF010000123 DAZKGF010000000
VERSION     DAZKGF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.123"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            96..986
                     /locus_tag="ACHOQV_003554"
     CDS             96..986
                     /locus_tag="ACHOQV_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631861.1"
                     /translation="MPPHKIRQEWLTLDEAAEVIRQHNITLLPADLIRHALHGDLSLS
                     VYFPSPVLLRPLNPPCRFACRFSKPPFPPSQCNKPGNRKVMANSPPCRRDPTLQLTTS
                     HLWNLPLIGIERLLLHQQLAKTLNRPCPNDTHCCNQIGIVVCDENDSYYQLCCRKSLR
                     ELLLELLATQNNLQEELHLLIGQLYGNGHNDLKSRLSLSVCYLQDTLPPDALFIIRRT
                     HLEAFLTRHQPESKERITSALSRLLWLACKHNPQLDDEMLEHPYKLLNIFECWAREEG
                     MHLPLSPETLKTALQRGAPP"
     gene            1125..1319
                     /locus_tag="ACHOQV_003555"
     CDS             1125..1319
                     /locus_tag="ACHOQV_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9631862.1"
                     /translation="MNSDRFLRLRQVEDKIGFGKSWIYRQIQLQQFPPSIRLNSRHVA
                     WLESEVDAWIHQRIRLTRDV"
     gene            1336..2619
                     /locus_tag="ACHOQV_003556"
     CDS             1336..2619
                     /locus_tag="ACHOQV_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFP9631863.1"
                     /translation="MCASHLLNRWLHGASNTGEQEMVQVSLEQIQPFSLNPRVTRNPG
                     YDVLKASILARGLDNPPVLTRRPGDEKYMLASGGNTRLAILNELWQETQDERYHRVCW
                     PFRPWPETLSPQQGEVQCLIGHLAESDLHNGLMFIERAEGVLHLRDLYLEAGIECPTQ
                     LALAEQLTRDGYPVSQSQISRMLQTVDWLLPCIPNALYGGLTRKVIDRLLSLRSAAEQ
                     VWVKLMPLERQPEFADLFSTALAIFNGEPEAVVPQLVQDELLGEMSCRSGMSWNTLLA
                     ELTDGQSKRQVLLGPPAEKVLWPPPPEVEKAPLPEPEPPTEPAAPDEPKPSPLVHTAQ
                     SLCACWGLDACIQPDDSAPAGFTLAPCHDFPHPAAQRCYQVLAALNGDTSTPMPALEL
                     LLSPDYNNAQIQQLMQLISLCRQRRLREVRHDTLS"
     gene            2603..3160
                     /locus_tag="ACHOQV_003557"
     CDS             2603..3160
                     /locus_tag="ACHOQV_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFP9631864.1"
                     /translation="MTPSVNQAVLTHIVQALKEGQIRYCESLGFSPQELCELTRLTAD
                     DILFLSNSAVQFITTHIDHEMLGRMLARMEQERLFQQRLEEALSLGASIEFINHYFGL
                     STPEVCARRRLIGLFVRQGRNNAPDEQVETLVWNEWQKTGVKKLDSPEALTAMMAMAR
                     EHDLSLTVIWNLLRQWTQEKLLHEE"
     gene            3150..4022
                     /locus_tag="ACHOQV_003558"
     CDS             3150..4022
                     /locus_tag="ACHOQV_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFP9631865.1"
                     /translation="MKSDDRLLFLGNPQQSVPKRLLLARNLSPRDKFAWQLLRLHARD
                     DGSGVFPSYNELQGWLSDQPEGEKASRSTVSNALMMLRITRWLSLCQRLRDEQSGRIT
                     GNLYALHDAPLTHADACRMDSEYLSLLTACSRHRNRRIRNIAIGLLLTLQQPVDGGRT
                     CTESPLKTAPGLNRGLSKKCPSLQLRLSNVAGAGGQPEPHCVHTGTDLNNKHQKRTPD
                     LHWPVDNPFSASERRAAERAMRNLDPALCQQVLDDCLWRIAAHDIHRPLAYLLATLEK
                     ARRGEFNQLRRRRR"
     gene            4182..4793
                     /locus_tag="ACHOQV_003559"
     CDS             4182..4793
                     /locus_tag="ACHOQV_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFP9631866.1"
                     /translation="MPGTTLFSDIRITLHTRVAARLWQAHPTGMLLCLALLRRLLRAE
                     EADDPWAAHWLKQLRIRLNLIAHLLKQKNRRLDHAFASLPGAIHTTQVSNPSPTEFIL
                     PLALFSPPGSRLLQQLIDYDLLVRRTLLAWHLGLITQAEKRDFIATIPRLMLQVFSFV
                     NRFRTTGVTRADVRANSPLAQSMAHKLGNLPKKMLTEIQRDVQ"
     gene            4783..5208
                     /locus_tag="ACHOQV_003560"
     CDS             4783..5208
                     /locus_tag="ACHOQV_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HFP9631867.1"
                     /translation="MFNDDDDERSARKSSLKGLLKAFKGKGELRALVQELQALRLQLQ
                     QNQPVLKQAVYELPGRNVPLILCSNPARSGVSYLRWRNLDNNRSGSVALQEAIQQPDA
                     SDELRDALMALEWRRHVLNTQLSVVMFMLRRIQPLTTLV"
     gene            5400..7205
                     /locus_tag="ACHOQV_003561"
                     /pseudo
     CDS             5400..7205
                     /locus_tag="ACHOQV_003561"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            7313..7495
                     /locus_tag="ACHOQV_003562"
     CDS             7313..7495
                     /locus_tag="ACHOQV_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011094427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631868.1"
                     /translation="MAKNTGDGYRKGSVTDRTQVQNPQNGNYTKRDTTTGRFIDQKQD
                     GTPFKGVAKEVDDRRK"
     gene            7779..8222
                     /locus_tag="ACHOQV_003563"
     CDS             7779..8222
                     /locus_tag="ACHOQV_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFP9631869.1"
                     /translation="MNDTHYFNLYTSGLGYLNRIREVTPKKGEPFLCCDIAALRGEAS
                     NAEHTWFDCRVSGKEAQKLVRRCIDACEQKRKVLIQFRLGDLYADTFVYSKGDRKGET
                     GVSLKAHLLHIGLIKIDGEEVYRAEREAEPQPAPAALPAEPVAQF"
     gene            8296..8676
                     /locus_tag="ACHOQV_003564"
     CDS             8296..8676
                     /locus_tag="ACHOQV_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631870.1"
                     /translation="MNTVLNSREEALDRGKLINLDDEALDAGLPLDLAITLPAHKHCL
                     EDRNDNAIRDLLKGVEMAAVVAFISHQPPQRTIRIDYRSVLTQKTNGSTQQQTVRLTL
                     RVHFSEQGIPIAATLGLMGERFPY"
     gene            8790..9446
                     /locus_tag="ACHOQV_003565"
     CDS             8790..9446
                     /locus_tag="ACHOQV_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilL"
                     /protein_id="HFP9631871.1"
                     /translation="MKRLLTALLLFIFNGVAASPATPEVVRYDRYLLVSTSPQQPPLE
                     QLTTLSVPPGFHPTVGEALQYLLRDSGWSLCLSDARRAQLYGLPLPLNQYHTGPLRLK
                     AALQLLAGPAWRMTADENRREVCFTSDHSLPQTVAYRHPVSLHPLGPLTAPFFLAGTV
                     QRGDIRLAVIVPPEALYLHQVVWLRIGEQWHGWRLARFDERRAVFRHGKRQLTLKVPE
                     "
     gene            9450..10124
                     /locus_tag="ACHOQV_003566"
     CDS             9450..10124
                     /locus_tag="ACHOQV_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFP9631872.1"
                     /translation="MKRLILLLLFTASTWAQPLQVQTSQNVGSALQPLTTQQQATVWG
                     ITPEEWQRYLTLKQQARGVWSPGLDPLTTLGVEADTDHERQRFAELLVRQEYQRLEKE
                     LAFQRAYDAAWQRLYPGLTPLKTTSASTSRVSLFVKDDCPPCDSLLRTLLAQQRPLDI
                     WLVGSEGDDNRVRRWAMAHGIDAKLVGEHSITLNHDAGRWLLLGQSKIPVALERKGEQ
                     WREVLP"
     gene            10103..10621
                     /locus_tag="ACHOQV_003567"
     CDS             10103..10621
                     /locus_tag="ACHOQV_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFP9631873.1"
                     /translation="MARGPALILLLPCLALAQPLPEAYLRTAEHFRVPPELLWAVTQT
                     ESGTNLSGRHLPWPWTLNISGTGYRYASREQACQALLVAIRRTSPKRIDVGLGQINIG
                     WHQAYFDSPCDALYPLQNLSLTAQLLRQRYDERPGSWLNAAARYHRPAGGAPAARYRK
                     LVSRHLQQASPP"
     gene            10618..12636
                     /gene="traD"
                     /locus_tag="ACHOQV_003568"
     CDS             10618..12636
                     /gene="traD"
                     /locus_tag="ACHOQV_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFP9631874.1"
                     /translation="MSRYALEALLRPPVEFYSTVIAGCGALICLTQAPLLMLTPWQAR
                     MMASALMLLALRRGWQGWQIVRYQRNLLRLPRYALTSRKIPVSRQRLFLGKGFRWQPI
                     HTQRLYDCLQPEAARWLRPGWCYRLARHIEKRSEHTLPGLARLLSADRWLNPVRPLPP
                     VGGNPWLHGVELKECDVTLPLSERPGHTLVLGTTRVGKTRLAELLATQDIRRGEVVIV
                     FDPKGDADLLKRMWTEAQRAGRGDRFILFHLAYPEFSARYNAIGRFGRISEVASRLTG
                     QLSGEGNSAAFREFAWRFVNIIARALVALGQRPDYQLISRHVVNIDALFIDYARIVLP
                     RHNTRSWEVVVQLAERVNDKNTPRHMLGRDPHVVALEQYLNAEKLYDPILDGLLSAVR
                     YDKTYFDKIVASLLPLLEKLTTGTLAALLAPDYHDLDDARPILDWQQAIRQKAVVYVG
                     LDALSDSVVAAAVGNSMFADLVSVAGHLYKFGLNDGLPGNSEKPPPINLHCDEFNELM
                     GEEFIPLVNKGGGAGIQVTAYTQTLSDIQARIGSAPKTGQVVGNFNNLIMLRVRETAT
                     AELLTKQLPQVEVRSRQVSSGVTDTPGGINGFTSNTCEQVTTRNVPLLDTAALIQLPK
                     GQAFALLEGGQLWKIRLPLPVADKQCPLPDSVAQLAAWMQARQQGLTE"
     gene            12633..13280
                     /locus_tag="ACHOQV_003569"
     CDS             12633..13280
                     /locus_tag="ACHOQV_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HFP9631875.1"
                     /translation="MSELRPPPSRPPQGPIGWLLSLAGRIIGLVIGTLVLRVVLELVG
                     LYFWWPQEGSRHVFQVMRQEKVELIIAVQPHPLGKEIRVLLEHGITRVNDVRHKAVSR
                     LFHEPVTVLAYTMISFALRLTWLVIMLPLLCLSAVTGLTEGLVHRDLRRFGSGLESIF
                     LHRYVIRCGSSVTTTLWVVYLAQPFSLPAMLVLLPAALWSGIAVWMAAGSFKRWL"
     gene            13346..13591
                     /locus_tag="ACHOQV_003570"
     CDS             13346..13591
                     /locus_tag="ACHOQV_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFP9631876.1"
                     /translation="MGVMSVRLNNNLSTQLEALSKATGRSKSWLANQAIEDYVAREAW
                     QVAEIEQAIQEADAGDFATSDEVDKLFQRLGVKPDGN"
     gene            13581..13865
                     /locus_tag="ACHOQV_003571"
     CDS             13581..13865
                     /locus_tag="ACHOQV_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9631877.1"
                     /translation="MEIKWLRKAIANLEAEFRYIAEDDPQAAERFVNEVWRLTQLLKE
                     QPAMGREGRVPETRELILHNYPYIIPYRVRNNIIQVLRVFHTHRRLPVNW"
     gene            13911..>14726
                     /locus_tag="ACHOQV_003572"
     CDS             13911..>14726
                     /locus_tag="ACHOQV_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631878.1"
                     /translation="MDKPIFNIINKKFIISVVTSSTFITGFTIAVIWFYLSNIDRLDI
                     FFDAVNLSSTLGIIFFFILLSISGFSLIIFISSFILILIYSGYENKLKVYSSITHRIS
                     TVCYQNSLFICCLLIIAYYIYYMTKWNGYIITAFSIISIFIHSYVTCAIRLFRKQKSR
                     LNNTVRYEKLSRKKGFNFWLSLQLIIPGVVQILPMMFLFNQLNFSEGTSDWYEMSILI
                     AITVVIVTIGILPGIIHINERQNGNKMTGIIVVLIFIPVATAALSVWYRPIPNM"
BASE COUNT         3248 a         4461 c         4006 g         3011 t
ORIGIN      
        1 acatacaaaa caaccagtta aaagaactca ccttacctcg ctttagttgg cttttttaga
       61 tagtactcat catatgtaac taataggagt tacccatgcc cccacacaaa attcgccaag
      121 agtggctgac gctggatgag gctgcagaag tcatccggca gcacaacatc acgttgctgc
      181 ccgccgacct gatccgccat gccctgcacg gcgatctgtc gctctcggtc tacttccctt
      241 cgcccgttct gctgagacca ctcaatccgc cttgccgctt tgcctgccga ttcagtaagc
      301 ctccttttcc accttcgcag tgcaacaaac ccggcaaccg caaagtgatg gcaaactcgc
      361 cgccgtgcag acgcgaccca actctgcaac tgaccaccag ccatctgtgg aatttgccgt
      421 taatcggcat tgagcgcctg ctgctccacc agcaactggc gaaaacgttg aatcgccctt
      481 gccccaatga tacccattgc tgcaaccaga ttggcatcgt ggtctgcgat gaaaacgaca
      541 gctactacca actctgttgc cgcaaatcgt tgcgggagct tttactggaa ctgctcgcca
      601 cgcaaaacaa cctccaggaa gagcttcatc tgctgattgg ccagctatat ggtaatggcc
      661 acaatgacct gaaatcccgg ctcagtctgt ccgtctgcta cctgcaggac acgctgcctc
      721 ctgacgcact gttcatcatc cgccgcactc atctcgaagc cttcctgacc cgtcatcagc
      781 cagaatcaaa ggagcggatc acctccgcgc tttcgcgttt gctgtggctg gcctgcaagc
      841 acaacccgca actggacgat gaaatgctgg agcatcccta caaactgctg aatattttcg
      901 aatgctgggc gcgtgaggaa ggtatgcacc tgccgctgag ccccgagacg ttgaaaaccg
      961 cactccagcg cggcgcaccg ccgtaacgcg atcaactaac cctttcaact aacaactctc
     1021 actaacccag ataaggattt ttactgcctg gcagggacgc tgctgctttg ataaccacgc
     1081 ctatcgtgct gatgtgaacg aatctggaca caggaggaat ggttatgaat agcgacagat
     1141 ttttacgctt acgccaggtg gaagataaaa tcggcttcgg taaatcgtgg atttatcgac
     1201 aaatccagct gcaacagttt ccgccgtcca ttcgactcaa tagccgccac gtcgcctggc
     1261 tggaaagcga ggtggacgcg tggatccacc agcgcatccg gcttacccgc gatgtctgaa
     1321 tgccggagga agattatgtg cgcttcacac ctgctcaacc gctggcttca cggcgccagc
     1381 aataccggag aacaggaaat ggtccaggtc agcctggagc aaatccagcc gttctccctc
     1441 aatccccgcg tcacccgcaa ccccggctat gacgtgctga aggcgtcaat tcttgcccgt
     1501 gggctggata acccgccggt actgacccgc cgccccggtg atgaaaaata tatgctcgcc
     1561 agcggcggaa atacgcgcct cgccatcctc aatgaactgt ggcaggagac gcaggatgag
     1621 cgttaccacc gcgtctgctg gccgttcaga ccgtggccgg aaacgctgtc accgcagcaa
     1681 ggcgaagtcc agtgcctgat cggccatctg gccgaaagcg acctgcataa cggattgatg
     1741 tttatcgaac gggcggaagg ggtcctgcac ctgcgcgatc tttacctcga ggcaggcatt
     1801 gaatgcccga cgcagctggc gctggcggaa caactcaccc gcgacggcta tcccgtcagc
     1861 cagtcgcaaa tcagccggat gctacagacc gtagactggc tgctgccctg catcccaaac
     1921 gcgctatatg gcggtcttac ccgcaaggtt atcgatcgcc tgctgtcact acgcagcgca
     1981 gcagaacagg tctgggtaaa gctgatgccg ctggaacgcc agcccgaatt tgccgacctg
     2041 ttcagcaccg ccctcgccat cttcaacggc gagccggaag ccgtggtacc gcaactggtg
     2101 caggacgaac tgctcggtga gatgagctgt cgcagcggca tgagctggaa tacgttgctg
     2161 gcggaactga ctgacggtca gagcaagcgg caggtgctgc tggggcctcc ggccgaaaag
     2221 gtcctctggc cgccaccgcc ggaagtggaa aaagcaccac taccagagcc tgagccaccg
     2281 actgaacccg ccgcgcccga tgagccaaag ccctcaccgc tggtgcatac ggcacagtcg
     2341 ctgtgtgcct gctggggact ggacgcctgt atccagccgg acgacagcgc tccggccggt
     2401 ttcaccctcg cgccttgcca cgattttccc cacccggcgg cacagcgctg ctatcaggtg
     2461 ctggcggccc tgaatggcga caccagtacg ccgatgcccg ccctcgaatt gttgctgtcg
     2521 ccggactaca acaacgctca gatccagcaa ctgatgcagc ttattagcct gtgccgccag
     2581 cggcggctgc gggaggtgcg ccatgacacc ctcagttaac caggcagtac tcacccacat
     2641 cgtccaggcg ctgaaggagg ggcagatccg ctactgcgaa tcgctcggct tcagcccgca
     2701 ggagctgtgc gagctgacgc gccttaccgc cgacgacatc ctgtttctca gcaacagcgc
     2761 ggtgcagttc atcaccaccc acattgatca cgagatgctg ggccggatgc tcgcgaggat
     2821 ggagcaggag cggctgttcc agcagcgtct ggaggaagcg ctgtcgctcg gcgcgtccat
     2881 cgagttcatt aaccactatt ttggcctttc cacgccggag gtctgtgccc ggcggcggct
     2941 gattggcctg tttgtccgcc aggggcgcaa caatgccccg gatgagcagg tggagacgct
     3001 ggtgtggaac gagtggcaga agacgggcgt gaaaaagctc gactcacccg aagcgctaac
     3061 cgccatgatg gcaatggccc gcgaacacga tctgtcgctg acggtcatct ggaacctgct
     3121 gcgccagtgg actcaggaga agctgctgca tgaagagtga cgatcggctg ctgtttctcg
     3181 gcaacccgca gcagagcgtg ccgaagcgcc tgctgctggc gcgcaacctc tccccgcgcg
     3241 acaagttcgc gtggcaactg ctgcgcctgc atgcccgcga cgacggcagc ggcgtcttcc
     3301 cctcctacaa cgaactgcag ggctggctct ctgaccagcc tgaaggcgag aaggcctcgc
     3361 gcagtaccgt cagcaatgca ctgatgatgc tgcggatcac ccgctggctg agcctgtgcc
     3421 agcggctgcg cgacgagcaa agcggcagga tcaccggcaa cctctacgcc ctgcatgatg
     3481 cgccactgac tcatgccgat gcctgtcgga tggacagtga atacctgtcg ctgctcaccg
     3541 cttgttcccg ccaccgcaac cgacgcatcc gaaacatcgc tatcgggctg ctgctcaccc
     3601 tgcaacaacc tgtggacggt ggacgaacct gcactgagtc tccattgaag accgcccctg
     3661 gtcttaatcg aggactcagt aaaaaatgcc cgagtcttca attaagactc agtaatgtgg
     3721 cgggggctgg cgggcagccg gagccgcatt gcgtacatac tggtacagat ttaaacaata
     3781 aacatcaaaa gcgtacgcca gacctgcact ggcctgtgga taatcccttc agcgccagcg
     3841 agcgccgcgc cgcagaacgc gcgatgcgca atctggatcc cgctctctgc cagcaagtgc
     3901 tggacgactg cctgtggcgg atcgccgccc acgatatcca ccgccccctc gcctatctgc
     3961 tggcaacgct ggagaaagcc agacggggcg agttcaatca gcttcggcgc aggcgcagat
     4021 agccctgatt ttatgcgcac cgcataaagc cggtgtacca actatcaatc aaacctcatt
     4081 catcggcgaa attaactttc tgcggaccag atgggtttcc cgtcgcctga ctcattgtgc
     4141 ggaccgacac tgcgggcatc tgaatccatg gagaatttcc gatgcctggc acaaccctgt
     4201 tcagcgatat ccgcatcacc ctgcataccc gcgtcgccgc ccggctgtgg caggcgcatc
     4261 ccactggcat gctgctctgt ctggcgttac tcaggcgtct gctgcgcgcc gaagaggccg
     4321 acgatccgtg ggccgcccac tggctgaagc aactgcgcat ccgcctgaac ctgatcgccc
     4381 acctactgaa gcaaaaaaac cgtcgtctgg accatgcttt cgccagcctg ccaggcgcta
     4441 tccacaccac gcaggtcagt aacccgtccc cgaccgaatt catcctgccg ctggcgctat
     4501 tctcaccgcc cggttcgcgc ctgctgcaac agttgattga ttacgatctg ctggtccgcc
     4561 gcaccctgct tgcctggcat ctcggcctta tcacccaggc ggagaagcgc gatttcattg
     4621 ccaccatccc acgcctgatg ctccaggtgt tcagcttcgt gaaccgcttt cgcaccactg
     4681 gcgtgactcg cgccgacgtg cgggcgaaca gtccgctggc gcagtcgatg gcgcataagc
     4741 tcggaaattt gcccaaaaag atgcttacgg agatacagcg cgatgttcaa tgacgatgat
     4801 gatgaacgct ccgcccgcaa aagctcgcta aagggccttt taaaggcctt taagggtaag
     4861 ggagagctgc gcgcgctggt acaggaatta caggcgctgc gcttgcagct gcaacaaaat
     4921 cagccagtgc tgaaacaggc ggtgtatgaa ctgccggggc gtaatgtacc gctgattctg
     4981 tgcagcaacc cggcgcgctc cggcgtcagc tatctgcgct ggcgcaatct ggacaacaac
     5041 cgttcgggga gcgtggcgtt gcaagaggcc attcaacagc cggacgcgag cgatgaactg
     5101 cgcgacgcgc tgatggcgct cgaatggcgg cggcatgtgc tgaatacgca gctttcagtg
     5161 gtgatgttta tgctgcggcg gatacaaccg ttaaccaccc tggtttaaaa ttttgcgcaa
     5221 agttaaaccc tgagtgtcac tttatcgatt attttaaact taactcatgc accttaaaac
     5281 aggcactgtg cgcttaatgc acgttttaag gtgcaatttt taccttctcc taccagtttc
     5341 ccctgtcgcc ttatccctcc ggcacaccat gctgaactcc ctttcaccgg agacagccca
     5401 tgcaacttta cctgtgcgaa aaaccctctc aggcacgcga catcgcccga attcttgacg
     5461 cccataaccg cagcgacggc tacctgcata acgccaccgt aaccatcacc tggtgtttcg
     5521 gccatctgct gagcatggcc gcgccggagg attacgatcc ggcgctgaaa cactggcgga
     5581 cggaacagct gccgtttatt cccccggcat ggcggttggt gatccgcccg gaggtgcata
     5641 agcagttccg tatcatcgaa aagctgctga aacaggcgga tgcagtggtg attgccaccg
     5701 acgccgatcg cgaaggcgaa accatcgccc gcgaaattct ggaacaatgc cgctggcgcg
     5761 gtccggtcca gcggctgtgg ctgtcggcgc tggatgaggc cagtatccac aaggcgctcg
     5821 ccactctgcg tcccggcgac agcacctttc cgctgtatca ggccggactg gcgcgcgccc
     5881 gcaccgactg gctggtcggc atcaacatga cccgcgcctg cacgctaatg aaccggcggc
     5941 acaagggcgt gctctccgtg ggccgggtgc agacgccgac cctgcgtctg gtggtggagc
     6001 gcgaccggga gatcgcccgc ttcacgcccc gcccctggtg gcgggtggat gcccgtttgc
     6061 atgcggaggg ttgttccttt ctggcgcagt ggcagccgga tagtgcgtac tgtgacgacg
     6121 aaagccgctg catccgcgaa tccgccgccc gtgaggccac agcacgcctg caacaagccg
     6181 gaaccgcgct ggtaatggac gtgacaaccc ggcgggagaa aacgccgcca ccgcaggcct
     6241 tcactctcag cgccctgcag caggcctgct caaaacagtg gggcatgggc gcacaggagg
     6301 ttctggatat cgcccagctc ctgtatgaaa cccataaagc caccacctac ccgcgcaccg
     6361 actgcggcta tctgccgctc tccatgcacg atgaagcacc acaagttctg gcggcgctgg
     6421 tcgccagcga cgcttcactc cagcccctcg ccacgcagct taatctgcag caacgctccc
     6481 ggttgtggga cgacagcaaa atcaccgccc accatggcat catcccgacc cgtaaaacgg
     6541 tagatctgac gcagatgaac gacagcgagc gcaaggtcta cggcctggta cgcagccatt
     6601 ttctggcgca gtttctgcca gaccacgaag tggataaaac ggacctgcgc ctgaactgcg
     6661 ccggggaaac gctgactgcc cgtggctcag tggtgattgt cagcggctgg cgacaactgt
     6721 ttttgcgcga aatggcaacg cactcaccgg aagaaacgca ggcacttccc gccctgcaac
     6781 agggacaaac ctgctaggtg gaacaggtgg acgttcgcgc ccgccagacc cagccaccgg
     6841 agcactacac cgaagggacg ctgattgccg cgatgaaaaa tgccgcccgt tttgtcagcg
     6901 atgaacgcct gaaacagcgg ctgaaagaaa acgcgggcat cggcaccgaa gccacccgcg
     6961 ccgggattat ccagacgctc cttaagcgcg gctatctgat taagcagcgt cgtttcctgc
     7021 tggcaacgga taccgccagc acgctgattg acgccctgcc ggaaacgctg aaagatccag
     7081 gcaccactgc actgtgggag caaatgctgg atgacatcgc gacggggaaa atgggactgg
     7141 aggcgttcct ggtgcagcaa cagcagaacg tgacagagct gattaagtcg ttacggaatg
     7201 agtaaataac ctgtttcctg catcacactt ttacttttcc ctgattgctc gttcaaattg
     7261 gcagtctgat gatcctctct gaggaatatt cctcattttt cggagtgtta agatggccaa
     7321 gaataccgga gacggttatc gcaagggctc tgttacagac cgcacacagg ttcagaatcc
     7381 gcagaatggc aactacacca aacgcgatac aacgacaggt cgctttatcg accagaagca
     7441 ggacggcact ccctttaaag gtgtcgccaa agaagtcgat gaccgccgga aataatattt
     7501 agctaacttt cagggcctta gttattaagg cctttttgca ttctgtactt ccaactttca
     7561 cgccgtaaaa acaattccac ctttcgtttc tccctaccga agattcaact aaacaggtca
     7621 accccaaaac ccgccatgca ttcctgaaac gcggaggcgc acagcgcgga atgcccgtac
     7681 tgccagtaac tcagaggcct tgcagccaat gagccaaacc cgaagcgggg cgttcccgcg
     7741 catggggcgc tccgcacctt caacacggag ataccaccat gaacgatacc cactacttca
     7801 acctgtacac cagcggtctg ggctacctca accggatccg ggaagtgacc ccgaaaaaag
     7861 gcgaaccgtt cctgtgttgc gacattgccg cactacgggg cgaagccagc aacgcggagc
     7921 acacctggtt cgactgccgc gtcagcggca aggaagcgca gaaactggtg cgccgctgta
     7981 tcgacgcctg cgagcagaaa cgcaaggtgc tgatccagtt ccgcctcggc gatctgtacg
     8041 ccgacacctt tgtctacagc aaaggcgacc gtaaaggcga aaccggcgtc agcctgaagg
     8101 cccatctgct gcacatcggg ctcatcaaaa ttgacggtga agaggtttac cgggcagagc
     8161 gcgaagctga acctcagcca gcgccagccg cactgccagc agagcctgta gcgcagttct
     8221 gacgcacagc attcaccctg ccggggaaat tccccgtcgg ggcgtttccc tggcctttta
     8281 ccggagagac gcacgatgaa taccgtattg aactcacgcg aagaagccct tgaccggggc
     8341 aaactgatta accttgatga cgaggcgctg gacgctggct tgccactgga tctggccatt
     8401 acccttccgg cccacaagca ctgtcttgaa gaccggaatg acaacgccat cagggacttg
     8461 ctgaaaggtg ttgaaatggc agcggtagtg gcctttatct cccaccaacc gccgcagcgc
     8521 accattcgta tcgattaccg ctcggtgctg acgcaaaaaa ccaacggcag cacgcagcag
     8581 caaaccgtca gattgacgtt acgcgttcat ttttctgagc aaggcatccc catcgccgcc
     8641 acgctggggc tgatggggga aagattccct tactgatctg attttgcgcc cggaataacg
     8701 ggcgctattt tttgacgcag caaacagttg tcggtagtcc ccagccctct catccggcac
     8761 gctcctgtaa aagcactgtg gaggatgcga tgaaacgact gctgacagct ctgctcctgt
     8821 ttattttcaa cggcgtggcc gcctcccctg caacgccaga agtcgtccgc tacgaccgct
     8881 atctgctggt cagcacgtca ccgcaacaac cgccactcga acagcttacc accctgtccg
     8941 ttccgcccgg ttttcacccg acagtcggtg aagcattgca gtacctgctg cgcgacagcg
     9001 gctggtcttt gtgtctctcc gatgcgcgcc gggcgcagct ttatggcctc ccgttaccgt
     9061 tgaaccagta ccacaccggc ccgctgcgcc tgaaggccgc cctgcaactg ctggcaggcc
     9121 cggcatggcg aatgaccgca gacgaaaacc gccgtgaagt ctgttttacc agcgaccatt
     9181 ccctccccca gacggtggct taccgtcatc ccgtgtccct ccaccctttg gggccgttaa
     9241 cggccccgtt ttttctcgcc ggaaccgtac agcgcggcga tatccgactg gcagtgatcg
     9301 tgccgccgga ggccctttat ctccatcagg tagtgtggct gcgtatcggg gagcagtggc
     9361 acggctggcg gctggcgcgg tttgatgagc gccgggcggt attccgccac ggtaaacgcc
     9421 agctgacgct gaaagtaccg gagtaagcga tgaaacgcct gattctcctg ttactgttca
     9481 ccgccagcac gtgggcgcaa ccgcttcagg tgcagaccag ccagaacgtg ggttcagccc
     9541 tgcaaccact gaccacgcag cagcaggcca ccgtctgggg catcacgcca gaggagtggc
     9601 agcgctatct cacgctgaaa cagcaggcgc gcggcgtctg gtcgccgggg ctcgatccac
     9661 tgaccacact cggcgtagaa gccgacactg accacgaacg ccagcgcttt gccgaactgc
     9721 tggtcaggca ggagtaccag cggctggaga aagaactcgc cttccagcgt gcttatgatg
     9781 ccgcctggca gcgcctgtat ccgggcctga cgccgctgaa aaccacctct gccagtactt
     9841 cccgcgtttc gctatttgtt aaagatgact gcccgccctg cgacagcctg ctgcgtaccc
     9901 tgctggcgca acagcgcccg ctggatatct ggctggttgg gagtgaaggc gatgataacc
     9961 gggtgcgccg atgggcgatg gcgcatggca tcgacgcaaa actggtgggt gaacacagca
    10021 tcacgcttaa ccacgacgcg ggccgctggc tcctgttggg gcagagcaaa atcccggtgg
    10081 cgctggaacg taagggggag caatggcgcg aggtcctgcc ctgatcctgc tgctaccctg
    10141 tctggcgctg gcacagccgc tgccagaagc gtacctgcgc accgcggagc atttccgcgt
    10201 gccgccggag ctgctgtggg ccgtcacaca aaccgaaagc ggcacgaacc tgagcggcag
    10261 acatcttccg tggccgtgga cgctcaatat cagcgggacc ggctaccgct atgccagccg
    10321 ggagcaggcc tgccaggcgc tgctggttgc catccgccgc accagcccaa agcgcattga
    10381 cgtgggactc gggcaaatca acatcggctg gcaccaggca tattttgact ctccctgcga
    10441 cgccctgtat ccccttcaaa acctgtcgct caccgcgcaa ctgctgcgtc agcgctacga
    10501 tgaacgtccc ggaagctggc tcaatgccgc cgcccgctat catcgtcccg ctggcggcgc
    10561 gcctgccgcg cgataccgca agctggtcag ccgccatctg caacaggcct caccgccatg
    10621 agccgctacg cgctggaggc gctgctgcgc ccacccgtgg agttttacag caccgtcatc
    10681 gctggctgtg gcgcattaat ctgcctgacg caggccccgc tgctgatgct gacgccgtgg
    10741 caggcccgca tgatggccag tgccctgatg cttctggccc tgcggcgcgg ctggcaggga
    10801 tggcaaatcg tacgctacca gcgtaacctc ctgcgcctgc cgcgctacgc gctcaccagc
    10861 cggaaaatcc ccgtcagccg ccagaggctg tttctcggta aaggctttcg ctggcagccc
    10921 atccacacac agcgtctgta cgactgcctg caaccggaag ccgcacgctg gctgcgcccc
    10981 ggatggtgtt accgcctggc aagacacatc gaaaaacgca gcgagcatac cctgcccggc
    11041 cttgcccgac tgctcagcgc cgatcgctgg ctgaacccag tacgtccgct gccacccgta
    11101 ggcggcaatc cgtggttgca tggggtggaa ctaaaagagt gcgacgtcac tctgccgctc
    11161 agtgaacgcc ccggccatac gctggtgctc ggcaccaccc gcgttggcaa aacccggctg
    11221 gcggaactgc ttgccactca ggacatccgc cgtggcgagg tggtgatcgt ttttgatccg
    11281 aaaggcgacg cggatctgct gaagcgtatg tggacagaag cgcagcgcgc cggacgcggc
    11341 gaccgcttta tcctgttcca tctggcgtac ccggagttct ccgcccgcta caacgctatc
    11401 gggcgatttg gccgcatttc ggaggtggcg tctcgcctca ctggtcagct ttccggcgaa
    11461 ggcaactcgg cggcctttcg cgagttcgcc tggcgctttg tcaacatcat cgcccgcgcc
    11521 ctggtggcgc tcggccagcg ccccgactat cagttaattt cccgccacgt cgtcaatatc
    11581 gacgccctgt ttatcgacta tgcccgcatc gtgctgccgc gccacaacac gcggtcatgg
    11641 gaagtggtgg tgcaactggc ggagcgcgtt aacgacaaaa acacgccgcg ccacatgctg
    11701 ggccgcgatc ctcacgtggt ggcgctggag cagtacctca acgcggaaaa attatacgat
    11761 ccgatcctcg atggcctgct ctcggcggtg cgctatgaca aaacctattt cgacaagatt
    11821 gtcgcctcac tgctgccact gctggaaaag ctgaccaccg gcacgcttgc cgccctgctg
    11881 gccccggatt accacgatct ggacgacgcg cgtccgatcc tcgactggca acaggcgatc
    11941 cgccagaagg cggtagtcta tgtcgggttg gacgcgctct ccgattcggt ggtcgctgcc
    12001 gccgtcggca attcgatgtt tgccgatctg gtgagcgtcg ccgggcatct gtataaattt
    12061 ggcctcaatg atggactgcc cggcaatagc gaaaaaccgc cgcctatcaa tctgcactgc
    12121 gatgagttta atgaactgat gggcgaggag tttattcccc tggtcaacaa gggcggcggt
    12181 gcgggcattc aggtcaccgc ctacacgcag acgttgagtg atattcaggc ccgtatcggc
    12241 agcgcaccaa agacaggcca ggtggtgggt aacttcaaca acctgattat gctgcgggta
    12301 cgcgaaaccg ccaccgccga actgctgacg aagcaactgc cacaggtgga agtgcgcagc
    12361 cgccaggtca gttcaggtgt caccgacacg ccgggcggca tcaacggctt taccagcaac
    12421 acctgcgagc aggtgaccac ccgcaatgta ccgctgctgg ataccgccgc gctgatccag
    12481 ctccccaaag gccaggcttt tgccctgctg gaaggcgggc agttgtggaa aattcgtctg
    12541 ccgctgcccg tagcggataa gcaatgccca ctaccagatt ctgtcgcgca actggcggcg
    12601 tggatgcagg cgcgccagca ggggctaacg gaatgagtga gctgcgaccg cctccgtcac
    12661 gcccaccaca gggaccgatt ggctggctat tgtcactggc cggacggatt attgggctgg
    12721 ttatcggtac gctggtgctg cgagtggtgc tggaactagt ggggctatat ttctggtggc
    12781 cgcaggaagg gagcaggcat gtttttcagg tgatgaggca ggagaaggtg gaactgatta
    12841 ttgcggtaca gccacatcct ttggggaaag agatacgagt actgctggag catggaataa
    12901 cccgcgttaa tgatgtcaga cataaggccg tgagtcggtt gttccacgaa cccgttaccg
    12961 tcctggctta taccatgatc agctttgcgc tgcgcctgac gtggctggtt atcatgttgc
    13021 cactgctgtg cctcagtgct gtaaccgggc tgacagaagg cctggtgcac cgggatttaa
    13081 gacggtttgg ctccgggctg gaatccattt ttctccaccg ttatgtcatc aggtgcggca
    13141 gttcagtcac caccacgctt tgggtggttt atctggcgca gccattttct ctgcccgcga
    13201 tgctggtttt acttcctgcc gccttatggt ccgggatagc ggtatggatg gcagcaggaa
    13261 gctttaaacg ttggttataa gttgctcaaa aaatcgcacc tcactacaat gtatacaatg
    13321 tgtacatgtg tatgaggcca ccaatatggg cgtaatgtca gtccgtttaa acaacaatct
    13381 ttccacgcaa ctggaagcat tatcaaaagc gaccggacgc tcaaagtcct ggctggcaaa
    13441 tcaggcaatt gaagattatg ttgcccggga ggcctggcaa gttgcagaga ttgagcaggc
    13501 cattcaggaa gctgatgccg gtgattttgc cacttcagat gaggtggata agctgtttca
    13561 acgtctggga gtaaagccag atggaaatta agtggctacg taaagccatt gccaatctcg
    13621 aagctgagtt ccggtatatt gctgaagacg atccgcaggc tgcggaacgg tttgtgaacg
    13681 aagtctggcg acttacgcaa ctgctcaaag agcaacccgc tatgggccgg gaaggaaggg
    13741 tacctgaaac acgtgagctc attcttcata attaccctta tatcattccc tatcgagtac
    13801 gaaataatat aattcaggtt ttacgcgtgt ttcataccca tcggcgctta cctgtaaatt
    13861 ggtgataaaa aacacaagac aagaaatttt ttagagatgg aattcaaacc atggacaaac
    13921 ctatttttaa tatcataaac aaaaaattca ttatttcagt agtaacctca tctaccttca
    13981 tcactggatt cacgatagca gttatatggt tttacctatc aaacattgat agattagata
    14041 tttttttcga tgctgtaaac ctcagttcaa ccttagggat tatattcttt tttattttat
    14101 tatcgatatc aggattcagc ctaataattt tcatctcatc ttttatttta atacttattt
    14161 actctggata tgaaaacaag ttaaaggtat attctagcat cacccatcgg atttctactg
    14221 tttgctatca gaatagctta tttatatgct gcctgctaat cattgcttat tacatttatt
    14281 acatgacaaa atggaatggc tacataatta cagcctttag tattatttcc atatttattc
    14341 acagctatgt tacatgtgcg attcggctat ttagaaaaca aaaatctcgc ttaaataata
    14401 cagtgcgata cgaaaaacta tccagaaaga aaggattcaa cttctggtta tccttacaac
    14461 ttataatacc tggagtagta caaatattac caatgatgtt tctgtttaac caacttaatt
    14521 tcagtgaggg tactagtgac tggtatgaga tgagcatact cattgccata actgtagtta
    14581 ttgttacaat tggcattctg cctggtatca tacatattaa tgaacggcaa aacggcaata
    14641 aaatgacagg aattattgtt gttttaatat ttatacctgt cgcaaccgct gcacttagtg
    14701 tttggtacag acctattccc aacatg
//
LOCUS       DAZKGF010000124        14541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZKGF010000124 DAZKGF010000000
VERSION     DAZKGF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.124"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(309..1181)
                     /locus_tag="ACHOQV_003573"
     CDS             complement(309..1181)
                     /locus_tag="ACHOQV_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP9631879.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            2522..4012
                     /locus_tag="ACHOQV_003574"
     CDS             2522..4012
                     /locus_tag="ACHOQV_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP9631880.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGESLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(4134..4706)
                     /locus_tag="ACHOQV_003575"
     CDS             complement(4134..4706)
                     /locus_tag="ACHOQV_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631881.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(4792..4998)
                     /locus_tag="ACHOQV_003576"
     CDS             complement(4792..4998)
                     /locus_tag="ACHOQV_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9631882.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            5855..6079
                     /gene="hha"
                     /locus_tag="ACHOQV_003577"
     CDS             5855..6079
                     /gene="hha"
                     /locus_tag="ACHOQV_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFP9631883.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVITRTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(6411..6608)
                     /locus_tag="ACHOQV_003578"
     CDS             complement(6411..6608)
                     /locus_tag="ACHOQV_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631884.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            6807..7376
                     /locus_tag="ACHOQV_003579"
     CDS             6807..7376
                     /locus_tag="ACHOQV_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFP9631885.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            7637..8038
                     /locus_tag="ACHOQV_003580"
     CDS             7637..8038
                     /locus_tag="ACHOQV_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP9631886.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTKHGHECPYVRPERREVRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            8026..8460
                     /locus_tag="ACHOQV_003581"
     CDS             8026..8460
                     /locus_tag="ACHOQV_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFP9631887.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            9235..9461
                     /locus_tag="ACHOQV_003582"
                     /pseudo
     CDS             9235..9461
                     /locus_tag="ACHOQV_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(9463..10245)
                     /locus_tag="ACHOQV_003583"
     CDS             complement(9463..10245)
                     /locus_tag="ACHOQV_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFP9631888.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            10548..11468
                     /gene="rbsK"
                     /locus_tag="ACHOQV_003584"
     CDS             10548..11468
                     /gene="rbsK"
                     /locus_tag="ACHOQV_003584"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9631889.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            11496..12812
                     /gene="fucP"
                     /locus_tag="ACHOQV_003585"
     CDS             11496..12812
                     /gene="fucP"
                     /locus_tag="ACHOQV_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP9631890.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAGQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            12824..13837
                     /locus_tag="ACHOQV_003586"
     CDS             12824..13837
                     /locus_tag="ACHOQV_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP9631891.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRYEYVKGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTTQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDQPEFRMIAPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            14259..>14541
                     /locus_tag="ACHOQV_003587"
     CDS             14259..>14541
                     /locus_tag="ACHOQV_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9631892.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVKLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPVTNSSGIGVELLPVEMTLDERPYPVFYAR"
BASE COUNT         3736 a         3238 c         3679 g         3888 t
ORIGIN      
        1 gtgaataaaa aagccggtat ccacagatgt ggataccggc aggtaaaggg ttacagtgcc
       61 ggagatccgg ctgacggaag tttctgaatt gtggttctgt tgttccctga atctcatgtc
      121 tgtgtgtatc tgcgcacgtt gctaacagga gttattatta gcataacgat cgatattaac
      181 gatcggtgaa atgggtatcg atcgttttat tctattatct agctattgca cggacaagag
      241 agacagtaac gaaaacagcg cttttgtgag cagacccaga ggcagggagg acatcatcca
      301 cgacaggttc agaagaaaat ccagttccat accgcacggg caacagagac caccgtgtca
      361 cgaacagcgc gcagggccgt acggacaaca gaggcaacac agacgctctc cgctgtgtca
      421 aatatccggt ccaccgcacc ggtaaactgt tcacgggcct gagagcggac agattccgtg
      481 cgcagcccgt cctgcagtcg ggtcatcagg ggactggcgg catggtcggg aagcgctgtc
      541 atgagcgcac tgaccagcgt atccagttcc cagccggtgc gggccgatac ggccacaacc
      601 ggatgtacgg gccggaacag acggaatacc gcctccgttt tctcacgaat gttctgttcc
      661 tgtgcgggag aaggctgaat gccggccata tcccattcat ggcagggctc cgttttgtcg
      721 gcctgcgtca ccacaaacag cacctgctga tgtccgcact gcaggatgtg tcgccagaaa
      781 tactcatcca cagacagggc ccggtcatcg gctttaatca gccacagcac caggtcaagt
      841 tcaggcagaa tgtcacggta cagggcctca tactctgcat ccctgtcccg gctctcgccc
      901 accccgggca ggtcagtgat aaccatgctg tgaccgtgac cactcagacg gaagcgccgt
      961 acttcccggg tgccggcgtg aacatcactg accggggtga cctccccctg aaacagtgca
     1021 ttacagagtg aggatttacc ggccccgctt ttacccataa tgccaatcac gggttcgtga
     1081 ctggtgagtt tacgcagatg ttccaggatg tgacgggaaa gtgagtaagg cagggaggaa
     1141 agcggttttt caattgcctc aatggcatca gacggattca tacatgtatt cccggtgaaa
     1201 aaagacaaaa ccccgcgcac cggaaaggtg gcgggggatc cacggagata atatctgtct
     1261 gaaattgttt tgaatcgatg ggattgttgc gtattaacat gaaggagatc aggacttatc
     1321 cgttactggg tgatctcctt ccatgaaaat acgcggttat tgttaaacag caggctgatt
     1381 actccgtaaa ggagaaaaat attcatcttg cagcctgatt atcaggtgtg tttatgttta
     1441 ctgaggtgag tatttttcag tatattgtcg gggcagtcag tgggagatat taaatttacg
     1501 tttagagtta tcttgcttct gctgtgtatt tttataagag tgcgcacgta atgatatctg
     1561 ttgataccat gttgttaagt gtttcccttc cgggtcatgc gtacttgcaa aatggcatct
     1621 gaatcgatca cggtttggcc aaaccgtgat cgaatagaga ggaatacgca cgtaatgatt
     1681 aaatgctttt gttacaccat tcttccggca tgaatattta tccagttccc ttttttattt
     1741 gtgtagtgat actgtcggaa tagcgttcag taatgtgaag tgtcctgtta aaaatcttta
     1801 ataaatgtgt caccctggtc aggaaatatc tgctgagata ttattagttg gtggtgtttt
     1861 tactgagtaa tagctggctt ttaattcctg aactgaaatg ctgtcgctcg tcagggttta
     1921 attaaaactg gatgatgatg tgaaaatgaa ggctggtttt tgtgtttgtc gaggaagtgt
     1981 tgtttttatc cattctcatc attggctggc tgcgattata cagaagtatt tgaagaaaaa
     2041 atggtgcatt ttatctgtgc cggcagggag gaggattttc ctgatatgtc agtattaaat
     2101 ggtttttgtt atttataaaa atgtcatcct gtccatagaa tattgtactg agtactcagc
     2161 attcagcgca ggaactgtca tcttctttat tttattcttc ttcaggaggg cagttttctg
     2221 aaagcgttac tccggggaaa ctgtttatgt ttattcccgg caggaaaccc gtacctgcaa
     2281 taccctggat gatgtttttt gctggtacgt tgttgtgtga aaattaatca acatgtgtgc
     2341 tccggagagc gcccgtagac gcacagattc attctgacat tctttagcga caaaataccg
     2401 ggtggttatt atttaacctg aaaatcgaca gaaattttta tcctgtattg attctgctgc
     2461 tatttttgct attagtagca atagttgcgg tactttatgt tctgtgtgtt gtgaggtcat
     2521 tatgccatac tttcagtatc ctgatgagtt tcccctgagc tcgctgcctc cactcattcg
     2581 tgatgccgtt attgaagctc agcaaatcac gcaggctccc ctggggcttg tggcagcatc
     2641 tgccctgggc gctgtttcac ttgtctgcca gaatctgatt gatgtctgca gactgaatac
     2701 actgcggggg ccggtttctc ttttcttact gacgcttgct gagtccggag aacgaaaaac
     2761 cgcagtggac aaattattaa tggagcccct gtaccagcag gagatgctgt tatattccag
     2821 acacaaaaat gaactgacga catggaaaaa taaggaagag cttttaaagg cgcagaaaaa
     2881 agcattgttg tcaaaactga ataaagaact ccgcaaaggc gcggatgagt cagaaacgct
     2941 cagacaactg gaagcgcttc agaaaaacag aggggaaaaa cctgtaagat ataaattcat
     3001 ttttaatgat gccacgacag cagcaattaa agatcagctt tgtggtcagt ggcgttcagt
     3061 gggtatcatg tctgatgaag ccggtataat atttgatggg tataccctgt ctgagctgcc
     3121 ttttattaac aaaatgtggg atggctccgt attgtcggtg gacagaaaga atgaacccga
     3181 acagatgatt gaaaatgcca gaatgacatt atccctcatg gtacaacccg gtctttttga
     3241 ccgatatatg gagcgaaaag gttctgttgc cagggacagt ggttttcttg cccgttgtct
     3301 aatcagtaaa cctgccacca cgcaggggaa acgctttatt aacggagcag tgaccccggg
     3361 agaatcatta acggcatttc atgaacgatt aatggaactg gccagaggca gtattgaaaa
     3421 atccagtaag gatgaacgtt attgtctgca tttttctcca gaagcgcaaa aaatatttat
     3481 tgatcactat aatgttctgg agcaggattt gtctccttcc ggacccttaa gccagtttcg
     3541 ggggcatgtt tcaaaaaaaa cagaaaacat tgcgagaatt gctgcgttat tgcagtattt
     3601 cagtcagggt gaagggaaaa tctctgccga tatcatgacg tctgctgtcg taataagttc
     3661 gtggtacaca gatgaatata aaaaattatt tgcgttacct gatgaatctg aactgcagct
     3721 gcaggacgcc cgggagctgc tcgactggct cattgaggag tgccgcggag aatgcccgcc
     3781 ccgtgtcagg aaaaattata ttctccagtg tggcccgggc cggttcagga acaggaaaaa
     3841 gctgaacgtc ctgctgaaca ttcttgcatc tcaactcagg ctctctgtcg tgcaggaagg
     3901 aaaaaccacc tatgtcctgc tttccgaaat aaacaatatc acgctgcctg aactgaatgc
     3961 gtgctacagc cagaaactga taagatggca tgagcaatcc tttggcttat aatgttccct
     4021 gttgctgaga cggattattc agtggtggta tcgcagtatg cccgtcaccc cccgcttctg
     4081 catgagggtg acggaggaaa agtaagtgtg cggaggcccg ggagcgttat gaatcaacag
     4141 gcgtataaca ggtactcgtc aatcatcagt tccagctcgc gcttttcccg tttgcgcgcc
     4201 acagcccagt tttcaagctc tgcatgtgat ttatgcttta actcttcagt ccagggctgc
     4261 atggagtggg cacacatcag atcataccag cacagccata tcagcgtgtg gtagagatca
     4321 cgatcgtcga aacgccggag caggctgttc agcctgtttg ccagccggtt ttcacccaga
     4381 cgatgtagct cactgagtaa aacgccgccc agattaaaat gtgtaagcag catgatttca
     4441 tgggtctgaa tgctggtcat taacccttcc tgaatggcag gaatgaacgg tggtgttccc
     4501 gggttgacga cctgatagag tccctgtggt gccactgaga tgatagtgcc tttttccggc
     4561 agaaaaaagt gggccagggt caggcgatga taaccatgaa gatgcggaga ccccattcca
     4621 tgagcgacga taagcctcgt cacgcttgtc atccatttct gatgctggat gagcagcagc
     4681 tcacaccggt tttcaagttt ttgcataata ttctctcctt tagcgcgcag caaaatgcct
     4741 ccgggggccg gatcatgcct ttgctgcggt tgaacggata acggtcagga attacggacg
     4801 ggactgtgca atacgcgcct gcagccaggt ttccacttca cttttcagcc atcgggagct
     4861 acggcccagt ttgatggggg caggaaaggc accatccttg atgagcttgt aaaaccattt
     4921 atcggtcagg ccggtcagtt gagtgataaa tgccatgtcg accatctggt catccatcag
     4981 tgaaactggg gtattcatga gaatgttcct tcggatgttg ttaatctcaa ccggcgttgg
     5041 tgatgaaaac aacgcaccac cttcaccggc ataacaaccg gggagaaacg taaaaaataa
     5101 tcgggaatgt gaccgtcagg agataaacgg ggggattgcg gtggctgagc cgtaccggta
     5161 tgtcgggtga ttgttgcgaa acgatgcttc cgcacgcgca cgggaggact ttcgtgagac
     5221 aaggggaaaa ctggcgttct gataattgca gagttgtttt tgaaggcggt tgtggtgcat
     5281 cagcctgtga cctgtgggca ggcgacaggt atcactggtg ggataagact gtctgattgg
     5341 tattataaat atataagaga gctcaaacac atgagttcat atatgtgaaa taaatctgtg
     5401 agtgtgatca gcagaccagt ctacccagta gcaacagatt gcgttgtact cacagggtta
     5461 tccctgataa ctggcgtata acctgcgtac ataaaacgta cctgcaatgc acctgaacag
     5521 agtgcattaa gcatccgcct gagcggtaaa cgtatccgat ctggtgagtg acagtctgag
     5581 agcattctca gtaatcgccc ccttgtatcc tggaaattgg ttaagagcga ttagatttgg
     5641 caaggtgtat gcatccagca gcttaacacc tcagctaact ggctggttac cttcaggttg
     5701 tcgcaatggc atcggacatc cggtgtcgga taactaaacg tagcgttaag gcgatattgc
     5761 tgagagaccg gttaagtggt ggcacgggag taatgagttt ggggagggta ccagaatgcc
     5821 agtcaggtgc ggtatgctgg cagtatcacg ttgattgcag cataaagtaa cgagagacga
     5881 atggaagtta aaaccagagc agttccgtcg ttgttcatcc cgggagacgc tggaaaaagt
     5941 gattacccgc acacgttata aacttacccc tgcggagctg gaagccttta actctgcggt
     6001 cgatcaccgg ctggcagaac tgacaatgaa caaactttac gatcgcgtgc cggcttccgt
     6061 ctggaaatat gtcacctgaa tcttgaatcc gcgctacgat gactctggtg ttatctgtgc
     6121 accggaagat cgggaacatt aagcagtata acggcaacaa agaattcgaa atcaaaaggg
     6181 aatggtgtac agaggggcag gcgttcaggc attaccggcc tgtatcttct gaaggggact
     6241 cgtatttgtc ggggctgagt attccgctcg attcgatcac ggattggcag aaacctgact
     6301 gtgattgatt caggtaccat tttgcaggga tccggtgcgt tactgtctgg cacaacaaac
     6361 gcagcgggtc gctctgcccc gccgattcct gataattcct cccctgtaac ctactgcaac
     6421 agtacaaata cactgacgcc tgaaacggta accagaatca gcaccagcag taacgtcagt
     6481 aaacagagca gacgatcggt cttccggtaa taaccgaagg ggtcgacagc gccacaatac
     6541 gggcaacaca ctgtattatg agtaattgcg tttccacagt ccctgcactt ctttttgcgt
     6601 atcacgatcg ctccctctcc acgccttatc cgtaacctgt tttttacatt aaaaaaacaa
     6661 cgccgggaat tcaatcgtca gtccggagac ggccgttcgg gttatcacag agtgcctgag
     6721 acagtgtcct gccggaggtc acctcagggg ctctgcgcat tttttacgac gtggctattc
     6781 cgtatgaacc atacggagat ttaaccatga cctacaaata caaccccttc tggcagcaac
     6841 gtattcgtga gacggtgcgg cacgcactgg atgttcatcc ccgcctgacg gcacttcggg
     6901 ttgatctgcg tctcccggat gtgccggcag caacggacgc ggctgtgata tcccgcttca
     6961 tcaatgccct gaaagcccga atcgacgctt atcagaaacg taagcatcgg gaaggtaaac
     7021 gcgtgcattc ctcgaccctg cattacgcct gggcccggga gttcggggag cttaaaggca
     7081 aaaaacacta tcacctgctg ctgctggtca accgggatac ctggtgtcgt gccggcgatt
     7141 accgcgctcc ggggtcactg gccgggatga ttaaacaggc ctggtgcagt gccctgggag
     7201 tggatgccgg acgttatgac acgctggcac attttcctga caagccagcg gtgtggctgg
     7261 aacgcgatga tgaaaccggc tttcagcagg tgctggaacg ttctggctat ctggcgaagg
     7321 agcataccaa agctcgcggc accggtgagc gcaattttgg ctgtagccgg agttgaacac
     7381 gacaggcgca ccaatacccg gcaaaccaaa agtatgggtt gttgtctggc ggcaacgatg
     7441 tatccactcg cccactgact gttttttacg tcagtgggcc attgtgtgac aaccacagtg
     7501 ctgtaacagc aaccagcctg ctttacccaa actccagtcc agattttaac tgaaccgcca
     7561 ttcacaccct gaccacgctg cccgtaccgg gacggctgtt gcctgcgtgt cgtctggcgg
     7621 ctgaggaaca tatactatgt acgcaaaatc ctttctcgct cttgatggca acggacgtct
     7681 gacgggggcc cgtaccgcac agactgcgcc ttacgaccgc tacacctgcc acctttgcgg
     7741 cagtgcactc aggtaccacc cgcaatacga cactgaacgt ccctggtttg aacacactga
     7801 cgacgggctg actaagcacg gtcatgagtg cccttatgtc agaccggaac gcagggaggt
     7861 tcgcttgatt aagcgtctgc agcaattcgt accggatgcc ttacccgtgg tgcgtaaagc
     7921 cagctggcac tgcagacaat gtcaccacga ttattatggg gagcagtact gcacaaactg
     7981 ccagaccgga ggattcagta ttccccggac aactcaggag gaaatatgcg aattctgaac
     8041 tgttatatgg cgaatgacag caaaggccat tttgttacgg cgaaagaagc tgcgaagcac
     8101 aaccgacagg acgttttgtg ctgtgtgtcc tgtggatgcc cgttaacact tcagcggggc
     8161 aatgacggac aaccaccgtg gtttgaacat gaccagatga ctgtcgctga aaaaatcctg
     8221 ctgcgatgta cctggcttga cccggcagag aaagaggccc gtcgtttgca tctgcagggc
     8281 atgacggttc cggattatac ggtgaaggtg agaaagtggt tttgtgtgat gtgtgacgaa
     8341 gattatgagg gggaaaagtg ctgcccacgc tgcggtactg gggtatacag cagggcgtgg
     8401 gggcggcagg aggtgccgtc ggaagatgcc agggctgata atccgttaca gaggctgtaa
     8461 tggttgcctc cggagcagtt tgcggtaatg ccgttcccgg gaatggattg ccagtgcggg
     8521 gactgtggag cttaattccg gtgttggtgg cctttccgtt gattttgagc caacagccct
     8581 cctgtgactg acaggctgcc tcgtcattcc attcgtatct ccagtaacag ggggttgtat
     8641 tgtattttaa tgtgccggtt caggcgtgcg tttcccggcg cgtctgcacc ggcttaacca
     8701 aattcaacag ggataaaata gtgtaaataa ccgaagtatt acttgcagaa tcgggggaag
     8761 catccggggt tttattcaac aggcatggga ggctccggct ctgacagaca ttgtactgtg
     8821 gaatgttgtt acaggttggg gcggcaaccg tgccaggaga ccatgagccg ttcacggcat
     8881 taaaggccac acttcggtcg tttacattcc ctgtggtggc aggcatcttc cgttaaggcg
     8941 tcagagcgtt accaacgtta tgttgtcatg gctcaccttt catagtcgat gcttatctgg
     9001 tgactgaaac tagccgcaga taaagagtgt gaagttgata ccatggagtg gagtgacgtg
     9061 caggcaggag attcgctcta tgcagggttg tgagatgaat tcaaaccggc tgtcagcgcc
     9121 agtaatattt gaagattcgt ccggttgtta tccagtgtgc attaaaaacc cagacattat
     9181 ggacaataca acggcttcca taacagggag gagcctgttt attatccggg cgtaatgact
     9241 aacctttgtg ggctttttcc cgtcgtgctt ttaacgttga aggcattttc tgcctgccga
     9301 ttcaggacag cgacaaaagc catatcagac aattggtcaa tgatgaccag cgtctggagt
     9361 agatgataag ccaaattgat aaactggaat atgtgatgaa agtgcaacgt aatcagtcca
     9421 atccgacgat gtttaacaag agtacggtgt tttttcagta agtcattcgt cttctgaatc
     9481 aggtgtaatg atctttacag aagcattccg gcagtgcgta acataatctg ccggtaacgg
     9541 acgctcgcta atcagaacat caaatgcaga gagcggtgcg aggctggcag aggctacttc
     9601 atcgaacaag gcgtggcggg cgagcaaaat tttccttagt ccacgggcca tcgctttacg
     9661 cttagtggca agatcttcgg gattaaacca gctgacgcca aagtgatcat gtacaccgct
     9721 ggcggaaata aatatttttc gcggattgag agagtctaat ggcgaagagt tactggcatc
     9781 gtaaaaagca tcacttctgg cacgatacgt accaccacaa agtattgctg tcacattagg
     9841 tttttcattc aacgcaacaa agacacgatg tgagtaacag atgccagtga aggtgattgc
     9901 atccgggatc atgcttataa cgagcggtat ctcctggcca ttatcaaaga agatcagatc
     9961 attttcatta accattccgg cggccagaat tgcaataggt aagtcatcac gatgatttct
    10021 cggaacgtcc tggattactg gcatggatgg tgcgggttta tgcaccatta caatatagcc
    10081 acccagtagg gtcagtggca gaggttcatc ttcctgatgg agatcgcgac gaatagtcat
    10141 tacagaaact tccagcattc gtgccgcgtc tttcaaatgg attctgtcgg ttttcttaag
    10201 tatttcaatc aaacgtcgga tacgctcttt ttgcttcgtt tccatcaact ctctccgtaa
    10261 aatgttataa aaataacatt atggcattag taaacattgt tttagttacc atactatacg
    10321 accgaccctt tgaaatgaaa agggtataga ctgctgaatt ttcggtgtta atcctgttag
    10381 gttgcttatg attagatagt gttggcctga gtgataagac tggaagtcta ttaaataaac
    10441 atcgggttga tgtgctaatc gcgatctcag tcacaaatga tgcaaaagta acatgctaat
    10501 gttatttaaa taccattgtt atgcgaaagt ttataagagg tagtgaaatg gacattgcag
    10561 ttattggttc caatatggtg gatctcatta cttacactaa tcagatgcct aaagaaggag
    10621 aaactctgga agcacctgcc ttcaaaatcg gatgtggtgg gaaaggtgct aatcaagcgg
    10681 tggcggcggc taaattgaat tcaaaagtcc tgatgctgac taaagtaggt gatgatattt
    10741 ttgcggataa tactattcgt aatctcgaat catggggaat taataccact tatgttgaga
    10801 aagtcccctg taccagcagt ggtgttgcac cgatctttgt taatgccaac tcgagcaata
    10861 gtatattaat aattaaggga gcgaataaat ttctttcccc ggaagatatc gatcgagctg
    10921 cagaagatct gaaaaaatgc aaattgatcg ttttacaact ggaagttcaa ctggaaacag
    10981 tttaccacgc gattgagttt ggtaagaaaa atggtattga agtgttatta aatccggctc
    11041 cggcattgcg tgagttagat atgtcttatg cctgcaaatg tgatttcttt atcccgaatg
    11101 aaacagaact ggaaatatta accggtatgt cggttgatac ttatgatcat attcgtttgg
    11161 ccgcacgttc gttagttgat aaagggctga acaatattat tgttaccatg agcgaaaaag
    11221 gcgcattatg gatgacccgc gatcaggaag tccatgttcc tgcattcaaa gtaaatgctg
    11281 ttgatactag tggcgctggt gacgcattta ttggttgctt ttcccattat tatgttcaaa
    11341 gcggtgatgt tgaagccgct ttgaaaaaag ccgctctctt tgccgctttc agcgtcacag
    11401 ggaaaggtac gcaatcatct tatccgagta ttgaacaatt taatgaattt ttaaccttaa
    11461 acgaataatc atcctgcaca gttaaaggta gcactatgaa cgataaaaac atcattcaga
    11521 tgcctgatgg gtatctgaat aagactccct tgttccagtt tattttgtta tcgtgtttat
    11581 tcccgctatg gggatgcgca gctgcattaa atgacatatt aatcactcaa ttcaaaagtg
    11641 tattttcgct aagtaacttt gcttcagcat tggtgcagag cgcattttat ggaggttatt
    11701 ttttaattgc aataccagca tcccttgtga ttaaaaagac cagttataag gtggcaattt
    11761 taattggcct gacgctgtat attggtggtt gtacactttt tttccctgcc tcgcatatgg
    11821 caacctacac tatgttcctt gcagctattt ttgcgattgc gattggcctg agtttccttg
    11881 aaactgcagc gaacacatac agttcaatga tcggtccaaa agcctatgcc acattgcggc
    11941 tcaatatcag ccagaccttt tatccgattg gtgctgcgtc tggtatttta ttgggcaaat
    12001 atctggtttt ctccgagggg gaaagccttg aaaagcagat gtcaggaatg aacgccgggc
    12061 aaattcacaa ctttaaggtg ctgatgttag agaacaccct tgaaccgtat aagtatatga
    12121 ttatgattct ggttgtggtc atggtgttgt tccttttaac ccgcttccca acctgtaaag
    12181 tggcacagac aagtcaccat aaacgcccgt cagcaatgga tacgctgcgc tatctggcta
    12241 gaaatccgcg ctttcgtcgc ggtattgtgg cgcagttcct ttatgtgggt atgcaggtcg
    12301 cagtatggtc atttaccatc cgtctggcgc tggagttggg cgatatcaac gaacgtgatg
    12361 cttcaaactt catggtctat agctttgcct gcttctttat tggcaagttt atagccaata
    12421 tccttatgac gcgttttaat ccggaaaaag tattgattct ctattccgtt ataggggcgc
    12481 tgttcctggc atatgttgca ctggctccaa gtttcagcgc ggtctatgtc gccgtcctgg
    12541 taagtgtttt gtttggccct tgctgggcga cgatctatgc aggaacactg gatacagttg
    12601 ataacgaaca tacagaaatg gcaggagcag tcattgttat ggctattgtc ggtgccgcgg
    12661 tggttccggc aattcaggga tatattgcag atatgttcca ctcactacag ctttcattcc
    12721 tggtttctat gctctgcttt gtctatgtcg gagtctactt ctggcgtgaa agcaaagtac
    12781 gtaccgcact ggctgaagtc acagcgtcct gaggagaacc gttatgagta cgcgtattaa
    12841 tttgtggcgg gcgctttttg gcgagaaacc tcgtatcttg ctggaaaata gtgattttac
    12901 tgtcacgtct tttcgatatg acagcggtgt cgaggggctt aaaattgcaa acagtcgtgg
    12961 acatttgatt attcttccct ggatggggca gatgatttgg gatgcgcaat ttgatggaca
    13021 cggcctcacc atgtgcaata tgtttcgcca gcctaaaccg gcgacagagg tgatagagac
    13081 ctatggctgt tttgcttttc attcaggttt gctggctaat ggctgcccat ctgcggaaga
    13141 tactcatctg ctacacggtg aaatggcctg tgccgcgatg gatgaagcat ggatggaatt
    13201 agagggagat atgttacgcc ttaccgggcg ttacgagtat gtaaaggggt tcgggcatca
    13261 ttatctggct cagccaacag tggtccttca caaatcctct acactgttcg atattaagat
    13321 ggccgtaacc aatctggctt ctgtagatat gccactacaa tatatgtgcc acatgaatta
    13381 tgcatatata ccaaatgcta cgttcagtca gaatataccg gacgaaatat taaggttgcg
    13441 tgaatcagta ccttcgcatg ttaatcccac cactcagtgg ctggcgttta atcaacggat
    13501 tatgcaggga gaagcctctc tgtcgacgct gaatcaaccg gagttttatg atccagaaat
    13561 tgtcttcttt gcagacaaac tggacgctta cacagatcag ccagagtttc gcatgatcgc
    13621 tcccgatggc actacgtttg ttactcgttt ctccagtgcc gaactaaatt atgtgacacg
    13681 ctggatcctt tataacggag agcaacaggt agccgccttt gcattacctg caacctgtcg
    13741 gcctgagggg tatcttgccg cgcaacgtaa tggcacgctg attcaggttg caccgcagca
    13801 aacgcgcact tttacagtca ccacaggaat tgaataacca ctcaaggcct gaacagcggc
    13861 gcgcttatcg ttaaggtaag cgcgtttttt tacccgccag gacaagacta tgatagcgtt
    13921 gcccttatag acgatcacat tatcgtccac tccaaatttg cgcaactact gtagagtggt
    13981 gtactccaca gcttctttga ggagcttaac ctccattgtt ttcttaccga gcaagcactg
    14041 caactctttt atttatttgt agtggtcaac aaaaactggt cacaatttca gtttttttcc
    14101 agtatctatt ttccgattcg ttcggcggta atccaccgtt atattcatga ggccggagtg
    14161 cactataaaa gtgtatgcgt aaaccgactg ctgtatgtag ccattaagcc agtattggta
    14221 acataggtac ccaccttttc aactagtgga cacctgttat ggaacaaaaa acattatcag
    14281 ctgaaccccg caggtcattt tcaaatgagt ttaaacttca aatggttaaa ctggctttac
    14341 aaccaagggg ttctgttgcc cgtattgccc gggagcatga tatcaatgat aatcttctgt
    14401 tcaaatggct caggctctcg ctgatcaaag ggcgtatatc acggcgtctt ccggtaacga
    14461 actcttctgg cattggcgtt gaattattac ctgtggagat gacgctggat gagcggcctt
    14521 accccgtttt ttacgcgagg t
//
LOCUS       DAZKGF010000125        14487 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZKGF010000125 DAZKGF010000000
VERSION     DAZKGF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.125"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..97
                     /locus_tag="ACHOQV_003588"
     CDS             <1..97
                     /locus_tag="ACHOQV_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HFP9631893.1"
                     /translation="NEIFRMVAIAFILLTVLVWFAKPPFTAKGVG"
     gene            complement(202..1533)
                     /gene="dsdA"
                     /locus_tag="ACHOQV_003589"
     CDS             complement(202..1533)
                     /gene="dsdA"
                     /locus_tag="ACHOQV_003589"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFP9631894.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLTQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLHNATHLVWATGGGMVPEEEMEQYLAKGRS"
     gene            complement(1551..1769)
                     /locus_tag="ACHOQV_003590"
                     /pseudo
     CDS             complement(1551..1769)
                     /locus_tag="ACHOQV_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2012..3007
                     /locus_tag="ACHOQV_003591"
     CDS             2012..3007
                     /locus_tag="ACHOQV_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9631895.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHISLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEDREGGRVTRIPCPLLIRCST"
     gene            complement(3015..4448)
                     /gene="cscA"
                     /locus_tag="ACHOQV_003592"
     CDS             complement(3015..4448)
                     /gene="cscA"
                     /locus_tag="ACHOQV_003592"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP9631896.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDSGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWQLG"
     gene            4664..5578
                     /locus_tag="ACHOQV_003593"
     CDS             4664..5578
                     /locus_tag="ACHOQV_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFP9631897.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDWDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            5650..6897
                     /locus_tag="ACHOQV_003594"
     CDS             5650..6897
                     /locus_tag="ACHOQV_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9631898.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCIAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(6977..7159)
                     /locus_tag="ACHOQV_003595"
     CDS             complement(6977..7159)
                     /locus_tag="ACHOQV_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9631899.1"
                     /translation="MWRDSILHSAMHSLMNSQARDTRDTSLNDDNVLEISEPRIRSTF
                     ERLYQNMNEAFGLGDY"
     gene            complement(7427..7627)
                     /gene="torI"
                     /locus_tag="ACHOQV_003596"
     CDS             complement(7427..7627)
                     /gene="torI"
                     /locus_tag="ACHOQV_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFP9631900.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(7751..8095)
                     /locus_tag="ACHOQV_003597"
     CDS             complement(7751..8095)
                     /locus_tag="ACHOQV_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631901.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHSFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            complement(8122..8289)
                     /locus_tag="ACHOQV_003598"
     CDS             complement(8122..8289)
                     /locus_tag="ACHOQV_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631902.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMLWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(8362..8646)
                     /locus_tag="ACHOQV_003599"
     CDS             complement(8362..8646)
                     /locus_tag="ACHOQV_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9631903.1"
                     /translation="MANLQLAVNGEYFDQMKSGEKTEEYRLCNDYWNKRLVNRKYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVNIGNE"
     gene            complement(8639..9703)
                     /locus_tag="ACHOQV_003600"
     CDS             complement(8639..9703)
                     /locus_tag="ACHOQV_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9631904.1"
                     /translation="MSKIDYQALRELAKQATQGEWVAFISTGTGTYAVHTPGDKRCED
                     VIKWTGFDGQKNAENNARYIAAFNPEVVQALLDENESMCRDLIARNGEIEGLRKLVAE
                     LEETKSKLNEQREYYEGVISDGSKRIAELEKSEEQLINERDHAESALADMYFAATGDR
                     PEWSNCFSFSDAVDAVVDRIADLEAKQPSPVVPDNTSESLAYAYKELTPEIMRGHIAV
                     FERYGIAPNDSSTTIQALRIALDGIERSDAMLHGAEPVSQTYKLNQLSGNSPVTPDGW
                     ISCSERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLY
                     LKEVTHWMPLPEPPQEVNRG"
     gene            complement(9700..10071)
                     /locus_tag="ACHOQV_003601"
     CDS             complement(9700..10071)
                     /locus_tag="ACHOQV_003601"
                     /inference="COORDINATES: protein motif:HMM:NF025303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631905.1"
                     /translation="MAAENAGIKSAIPESRDIEDDNDNMDDVSLAEDFGFNHAIERMR
                     RQIPETPTTDAFLAEVRAQGVDAAIEAAKNLVAQEYEYKDFKAAQSDCCMHPGSDLVG
                     KVEMTEWLVDFAAQLRKGGSQ"
     gene            complement(10069..10269)
                     /locus_tag="ACHOQV_003602"
                     /pseudo
     CDS             complement(10069..10269)
                     /locus_tag="ACHOQV_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10266..10430)
                     /locus_tag="ACHOQV_003603"
     CDS             complement(10266..10430)
                     /locus_tag="ACHOQV_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFP9631906.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRSGKK"
     gene            complement(10447..10761)
                     /locus_tag="ACHOQV_003604"
     CDS             complement(10447..10761)
                     /locus_tag="ACHOQV_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631907.1"
                     /translation="MKKTPWEKWEVDFLREVAATMPVEVIAEKLERTEKAVMAKATRI
                     GADIVSRLRGRRWTRAEVSLFGKFSAEEIAIATCRSIYSVRAMRYKLKKLDEERAGIR
                     IN"
     gene            complement(10773..11255)
                     /locus_tag="ACHOQV_003605"
     CDS             complement(10773..11255)
                     /locus_tag="ACHOQV_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFP9631908.1"
                     /translation="MSKLYEIANEYAKLMDSDLEPEMIADTIEGMEGEFTDKIEQLLA
                     IIKNESGYAERLKEEAKSLNERAAVIQNKIDSIMAYIASSLEMVGKKKIRAGIHQVTI
                     RKPSETVEIIDSSAIPPEYVEFETTIKADKLAIKHQLKAGINIPGAQLKVGKPSLLIK
                     "
     gene            complement(11239..12141)
                     /locus_tag="ACHOQV_003606"
     CDS             complement(11239..12141)
                     /locus_tag="ACHOQV_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFP9631909.1"
                     /translation="MSNIVEFVKQQEQLFCGALTEQTVTWAKESQFAIQYFQKNDYLA
                     KTALANPTSAQNAIINVAAIGITLNPASKLAYLVPRDGMVCLDISYMGLLHLAQSTGS
                     IKWGQCKLVYSNDTYESNGLDSAPTHKYNAFGERGSIVGGYCTVKTADGDYLTEEMSL
                     AEIKAVEATSKAKNGPWKTFWEEMARKTIVKRASKYWPKAQRLDNAIHLLNEDEGMHQ
                     EPVMPHKSEEDIREDERKRQQEITDKAQLLCDEMAQAENMDDLKRYFAEAYRLTSGMK
                     LQQNIQAIYIECKAKLEVASEQTI"
     gene            complement(12138..12446)
                     /locus_tag="ACHOQV_003607"
     CDS             complement(12138..12446)
                     /locus_tag="ACHOQV_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631910.1"
                     /translation="MILTLNDKREISQIIASFTDEDYERINSEVDRLCKRCDPISEML
                     RLYKPDEHTKDAIDWLEDDDCNYQEKAAEWFWDAITERVKAEYAFAIFKRRHIYGEAA
                     "
     gene            complement(12531..12695)
                     /locus_tag="ACHOQV_003608"
     CDS             complement(12531..12695)
                     /locus_tag="ACHOQV_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631911.1"
                     /translation="MKKPTYEELEEALKELRRMAFARRTNSHNCGPFQYSDLCEDIIE
                     VTQLIKVVKQ"
     gene            complement(12692..12862)
                     /locus_tag="ACHOQV_003609"
     CDS             complement(12692..12862)
                     /locus_tag="ACHOQV_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631912.1"
                     /translation="MQQFAIAGAASVRPFNPILSVQHSRKNILTGADFKQPRVKSLLD
                     RLVEFLNQKVQP"
     gene            complement(13059..13385)
                     /locus_tag="ACHOQV_003610"
     CDS             complement(13059..13385)
                     /locus_tag="ACHOQV_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631913.1"
                     /translation="MKVLMVYENVPESTEIYIFDANEDEVNDLKLSHGNYTNANCDES
                     IEKALSRVLVRISDPEHCDDDWLSYCGAVKTDAGKWSKSKVDNSTPIIMKDSDIEMVI
                     ITGMIM"
     gene            complement(13439..13888)
                     /locus_tag="ACHOQV_003611"
     CDS             complement(13439..13888)
                     /locus_tag="ACHOQV_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631914.1"
                     /translation="MEEQANKILVELLQKASNGIDAAVSFSQAQIPDVVHQLLLWNMV
                     DSLIKTLIAILTIPLVFWFMKKQCQRVETGKIGDEGYSWERGNPKYRPTMVWDSKGDI
                     NLLIMPLVGVLTLWGIFIIGVVTNMTWLKIWLAPKLYLIEYAASLVK"
     gene            complement(13897..14256)
                     /locus_tag="ACHOQV_003612"
                     /pseudo
     CDS             complement(13897..14256)
                     /locus_tag="ACHOQV_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
BASE COUNT         3419 a         3467 c         3301 g         4300 t
ORIGIN      
        1 aaatgagatt ttccgtatgg tggctattgc ttttatctta cttacggtat tggtttggtt
       61 tgcgaaaccg ccgtttacag cgaaaggcgt tgggtgatgg tataaagaga gaattatagc
      121 aggtaagacc ggggatctta agtctgcgcc atcggcagca tcgctcaccc agggaaagga
      181 tggcgatgct gcgttgaaac gttatgaacg gccttttgcc agatattgct ccatctcttc
      241 ttccggcacc attccacctc ccgtcgccca caccagatga gtggcattat gcagttgttc
      301 tgcgctgaaa ccgtgcatct gttggtaact tactgatgca cacacgcgct gaggtccggc
      361 catacccgcc agtgccgaag gttcaagacg aataccttct tcctgcgtca gccagccaag
      421 catgtcatac atggtttgat cgctaagggt atagaagcca tccagtaaac gctccattgc
      481 ccgcccgaca aagcctgatg cgcgaccaac tgcaaggcca tccgctgcgg taaggttatc
      541 gataccaata tcctgaacag aaatctgatc gtgtaatcct gtatggacgc ctaacagcat
      601 acaaggggag tgcgttggtt cggcaaaaaa gcagtgaaca tgatcgccaa atgccagttt
      661 aagcccgaat gcgacgccac caggaccacc gccaacacca cacggcagat agacaaacag
      721 agggttatca gcatcgacaa tacgaccttg ctcggcaaat tgcgctttaa gacgctggcc
      781 agcgacggaa tacccaagga acaacgtgcg ggaattctcg tcatcaataa agaaacagtt
      841 cgggtcagat tgcgctgctt tacgtccttc ctcgactgca acaccataat cttgctcata
      901 ttccacgacc gtaacgccat ggctgcgcag tttcgctttt ttccatgccc gggcatcagc
      961 agacatatgt actgtcacct taaagccaat gcgggcgctc ataatgccga ttgataaccc
     1021 cagatttccg gttgagccca cagcaatgct gtattgacta aagaactgtt taaactccgg
     1081 agaaagcagt ttgctgtagt catcttcaag cgtcagcaac cccgcttcca gagccagttt
     1141 ttctgcatgt gccaggactt cataaatccc gccgcgtgct tttatggagc cggaaatggg
     1201 caaatggcta tcttttttca gtagcagttg cccgctgatc ggttgttgat attccttttc
     1261 cagccgtttt tgcatagccg gaatggcaac cagttctgat tcaataatcc ccccagtagc
     1321 agcagtttca ggaaatgctt ttgccagata gggtgcaaaa cgggataagc gcgcatgggc
     1381 gtcctgaaca tcctgttcgg tcaggccaac ataaggtaaa ccttcagcca atgaggtcgt
     1441 gccaggatta aaccaggtgg tttctttaag agcaaccaga tcctttacca acggatactg
     1501 ggcgatgagc gagttcattt tagcgttttc catagtacgt ctctttgcgc ttagatgata
     1561 aatgacagca ggaatgtgcc cgccagagcg acgactgaag cgataaatgt cgctgtcgta
     1621 tagtatttaa atgtttcatt gagcgtagcg ccgcaatatt gcttcaccag ccagaaaagc
     1681 gagtccgtaa cgatcgtgca gccaatcgca cctgaaccga tagcaatcgc aataatttcc
     1741 ggactgatgt cgggatacag cggcagcaca atgatccata tttatatttt cttctccagg
     1801 ttggcgatgc cagttggcat gttttaccta cttgcctaca tggttactcg ttggcgagaa
     1861 cagaggcgga attgggtaca attgtccggg aattattgtt attttttatt gtattgccgt
     1921 gtcgggtgat agggcgttga gaatatcgtt ggatctcgcc agtgacgtct gtttctgcta
     1981 cagtgcccgt tttacggcaa acggcttggg tatggcttca ttaaaggatg tcgcacgcct
     2041 ggcgggagtg tcgatgatga cagtctcccg ggtgatgcat aatgcagaat ctgtgcgtcc
     2101 tgcaacgcgt gaccgcgtat tgcaggcaat ccagaccctg aattatgttc ctgatctttc
     2161 cgcccgtaag atgcgcgctc aaggacgtaa gccgtcgact ctcgccgtgc tggcgcagga
     2221 cacggccacc actcctttct ctgttgatat tctgcttgcc attgagcaaa ccgccagcga
     2281 gtttggctgg aatagttttt taatcaatat tttttctgaa gatgacgctg cccgcgcggc
     2341 acgtcagctg cttgcccacc gtccggatgg cattatctat actacaatgg ggctgcgaca
     2401 tatctcgctg cctgagtctc tgtatggtga aaatattgta ttggcgaact gtgtggcgga
     2461 tgacccagcg ttacccagtt atatccctga tgattacact gcacaatatg aatcaacaca
     2521 gcatttgctc gcggcgggct atcgtcaacc gttatgcttc tggctaccgg aaagtgcgtt
     2581 ggcaacaggg tatcgtcggc agggatttga gcaggcctgg cgtgatgctg gacgagatct
     2641 ggctgaggtg aaacaatttc acatggcaac aggtgatgat cactacaccg atctcgcaag
     2701 tttactcaat gaccacttca aatcgggcaa accagatttt gatgttctga tatgtggtaa
     2761 cgatcgcgca gcctttgtcg cttatcaggt tctcctggcg aagggggtac gtatcccgca
     2821 ggatgtcgcc gtaatgggct ttgataatct ggttggcgtc gggcatctgt ttttaccgcc
     2881 gctgaccaca attcagcttc cacatgacat tatcgggcgg gaagctgcat tgcatattat
     2941 tgaagatcgt gaagggggaa gagtgacgcg gatcccttgc ccgctgttga tccgttgttc
     3001 cacctgatat tatgttaacc cagttgccag agtgctccat gttgcagcac agccactccg
     3061 tgggaggcat agagcgacag ttcccgttct tctggctgcg gatatattcg gctactcatc
     3121 accgcctccc cgtcgttaat aaatacttcc acggatgatg tatcgataaa tatccttagg
     3181 gcgagcatgt caccctgcgg gaggggaata ctacggtagc catctaaatt ctcgtgtggg
     3241 taataccgcc acaaaacaag tcgctcagat tggttatcaa tatacagccg cattccagtg
     3301 ccgagctgta atccgtaatg ttcggcatca ctgttcttca acgcccactg cagctgaatc
     3361 tcaactgctt gcgcgttttc ctgcaaaaca tatttattgc tgattgtgcg gggagagata
     3421 gattgatgct gctggcgtaa cgactcagct tcgtgtaccg ggcgttgtag aagtttgcca
     3481 ttgctctctg atagctcgcg cgccagcgtc atgcagcctg cccagccttc acgttttgag
     3541 ggcattggcg attcccacat atccatccag ccgataacaa tacgccgacc atccttcgct
     3601 aaaaagcttt gtggtgcata aaagtcatgc ccgctatcaa gttcagtaaa atgcccggat
     3661 tgtgcaaaaa gtcgtcctgg cgaccacatt ccgggtatta cgccactttg aaagcgattt
     3721 cggtaactgt atccctcggc attcattccc tgcggggaaa acatcagata atgctgatcg
     3781 ccaaggctga aaaagtccgg acattcccac atatagcttt cacccgcatc agcgtgggcc
     3841 agtacgcgat cgaaagtcca ttcacgcaat gaactgccgc gataaagcag gatctgcccc
     3901 gtgttgcctg gatctttcgc cccgactacc atccaccatg tgtcggcttc acgccacact
     3961 ttaggatcgc ggaagtgcat gatgccttct ggtggagtga ggatcacacc ctgtttctcg
     4021 aaatgaatac catcccgact ggtagccaga cattgtactt cgcgaattgc atcgtcatta
     4081 cctgcaccat cgagccagac gtgtccggtg tagataagtg agaggacacc attgtcatcg
     4141 acagcactac ctgaaaaaca cccgtctttg tcattctcgt ctcctggcgc tagcgcaata
     4201 ggctcatgct gccagtggat catatcgtcg ctggtggcat gtccccagtg cattggcccc
     4261 cagtgttcgc tcatcgggtg atgttgataa aacgcgtgat aacgatcgtt aaaccagatt
     4321 aggccgtttg gatcgttcat ccacccggcg ggaggcgcga ggtgaaaatg gggatagaaa
     4381 gtgttacctc ggcgctcgtg aagttttgct agtgcgtttt gcgccgcatg taatcgagat
     4441 tgcgtcattt ttatcatcct ggttaagcaa attgggtgaa ttgttaacgt taacttttat
     4501 aaaaataaag tcccttactt tcataaatgc gatgaatatc acaaatgtta acgttaacta
     4561 tgacgttttg tgatcgaata tgcatgtttt agtaaatcca tgacgatttt gcgaaaaaga
     4621 ggtttatcac tatgcgtaag tcagatgaat ttaagggaaa aaaatgtcag ccaaagtatg
     4681 ggttttaggg gatgcggtcg tagatctctt gccagaatca gacgggcggc tactaccttg
     4741 tcctggcggc gcgccagcta acgttgcggt gggaatcgcc agattaggcg gaacaagtgg
     4801 gtttataggt cgggtcggtg atgatccttt tggtgcgtta atgcaaagaa cgctgctaac
     4861 tgagggtgtc gatatcacgt atctgaagca agatgaatgg caccggacat ccacggtgct
     4921 tgtcgatctg aacgatcaag gagaacgttc atttacgttt atggtccgcc ccagtgccga
     4981 tcttttttta gagacgacag acttgccctg ctggcgacat ggcgaatggt tacatctctg
     5041 ttcaattgcg ttgtctgccg agccttcgcg taccagcgca tttactgcga tgacggcgat
     5101 ccggcatgcc ggaggttttg tcagcttcga tcccaatatt cgtgaagatc tatggcaaga
     5161 cgagcatttg ctccgcttgt gtttgcggca ggcgctacaa ctggcggatg tcgtcaagct
     5221 ctcggaagaa gaatggcgac ttatcagtgg aaaaacacag aacgattggg atatatgcgc
     5281 cctggcaaaa gagtatgaga tcgccatgct gttggtgact aaaggtgcag aaggggtggt
     5341 ggtctgttat cgaggacaag ttcaccattt tgctggaatg tctgtgaatt gtgtcgatag
     5401 cacgggggcg ggagatgcgt tcgttgccgg gttactcaca ggtctgtcct ctacgggatt
     5461 atctacagat gaaagagaaa tgcgacgaat tatcgatctc gctcaacgtt gcggagcgct
     5521 tgcagtaacg gcgaaagggg cgatgacagc gctgccatgt cgacaagaac tggaatagtg
     5581 agaagtaaac ggcgaggccg ctcttatctc taaataggac atgaattttt taacgacagg
     5641 caggtaatta tggcactgaa tattccattc agaaatgcgt actatcgttt tgcatccagt
     5701 tactcatttc tcttttttat ttcctggtcg ctgtggtggt cgttatacgc tatttggctg
     5761 aaaggacatc taggattaac agggacggaa ttaggtacac tttattcggt caaccagttt
     5821 accagcattc tatttatgat gttctacggc atcgttcagg ataaactcgg tctgaagaaa
     5881 ccgctcatct ggtgtatgag tttcattctg gtcttgaccg gaccgtttat gatttacgtt
     5941 tatgaaccgt tactgcaaag caatttttct gtaggtctaa ttctgggggc gctctttttt
     6001 ggcctggggt atctggcggg atgcggtttg cttgacagct tcaccgaaaa aatggcgcga
     6061 aattttcatt tcgaatatgg aacagcgcgc gcctggggat cttttggcta tgctattggc
     6121 gcgttctttg ctggtatatt ttttagtatc agtccccata tcaacttctg gttggtctcg
     6181 ctatttggcg ctgtatttat gatgatcaac atgcgtttta aagataagga tcaccagtgc
     6241 atagcggcgg atgcgggagg ggtaaaaaaa gaggatttta tcgcagtttt caaggatcga
     6301 aacttctggg tttttgtcat atttattgtg gggacgtggt ctttctataa catttttgat
     6361 caacaactct ttcctgtctt ttatgcaggt ttattcgaat cacacgatgt aggaacgcgc
     6421 ctgtatggtt atctcaactc attccaggtg gtactcgaag cgctgtgcat ggcgattatt
     6481 cctttctttg tgaatcgggt agggccaaaa aatgcattac ttatcggtgt tgtgattatg
     6541 gcgttgcgta tcctttcctg cgcgctgttc gttaacccct ggattatttc attagtgaag
     6601 ctgttacatg ccattgaggt tccactttgt gtcatatccg tcttcaaata cagcgtggca
     6661 aactttgata agcgcctgtc gtcgacgatc tttctgattg gttttcaaat tgccagttcg
     6721 cttgggattg tgctgctttc aacgccgact gggatactct ttgaccacgc aggctaccag
     6781 acagttttct tcgcaatttc gggtattgtc tgcctgatgt tgctatttgg cattttcttc
     6841 ctgagtaaaa aacgcgagca aatagttatg gaaacgcctg taccttcagc aatatagacg
     6901 taaacttttt ccggttgttg tcgatatctc catatccctc aaccggaaaa taataataca
     6961 aaaatgctta cccctgctaa taatcaccta atccaaacgc ctcattcatg ttctggtaca
     7021 gtcgctcaaa tgtactccgg atacgcggtt cgctgatttc caggacattg tcgtcattca
     7081 gcgacgtgtc ccgtgtatca cgggcctgcg aattcatcaa ggaatgcatt gcagagtgaa
     7141 gtatcgagtc acgccatatt tcgctatcag gattctgtat gatggttaca tcgcccggcc
     7201 cagggctgtt tagtcatcag cgctttctga cagtgctgag atttcaacct gttgcagtaa
     7261 aaatgagtag atatagggca agtgtgctgc caaacccatc ttttacgggg tgaaggtaga
     7321 tttggtttga agggtatctg gtgtcccctg cagacatcta cttgaagcga caggggattg
     7381 attggaatgg tgttttttag atgtgagaaa tattttaccc gctattttac ccattggcgc
     7441 ggcttaagag cttatttttg aattcacaat ggtcacgata taaccatctt gctcgcccgt
     7501 ggataacttt ggcttttggc aggtcgccgg acttaatccg gtcatagatg aaggttttac
     7561 caaagccagt atcagccatg atgaatttca aatcaaccag tgaatcaggt tgtagttcgt
     7621 gttgcatgag tgctatctcc gaatatggaa tcgaacctgc aaatcaggta ataaaaaaac
     7681 cgtattgatg cggcgatggt aggtctggat atcttgataa atgaaaatgc ctcatcgagt
     7741 gtgaggcggg ttagtccttg cgtagctcgc tgattcttct gtaagtctct ggtgctttgt
     7801 tcccgtacgt cttcatttca gacttcaaca gagcaacgag tgaatcccat tcgttgagga
     7861 ttcctttgaa tgccggaacg cgctttgcaa ccttgtcgaa tgaatctctg atttctggaa
     7921 tctgctcaac aagtgcaacg catcgtctga aatcggctgc gtcatgtgga gcaccgaagc
     7981 tatgaccata gatattcttt ttcagtccac atgcgattga ggcaagagtt gcgctactga
     8041 tgccgacatc gccagtcgat tgccatttca aaaccttcat agccaaatct gacatttctt
     8101 gtctccaata aaaaaccgcc atcaggcggc ttggtgttct ttcagttctt caattcgaat
     8161 attggttacg tctgcatgtg ctatctgtgc ccatagcatc cagtggtcat agcagtcgtt
     8221 gatgttctct gcttcgataa ctctgttgaa tggctctcca ttccattctc ctgtgactcg
     8281 gaagtgcatt tatcatctcc ataaaacaaa actcgccgta gcgagttcag ataaaagaaa
     8341 tccccgcgaa tgcgaggatt gttattcatt gccgatattc acctttatcg cgaacacctt
     8401 taccggttta tcgccgaagt gcggatgtgt gattgtcttg atttcatatc cgtcatacgg
     8461 gacgtcaatt ctgcggctgg aatcgtcgcg cttcggatat ccctttgtga taatcaggcg
     8521 gtcatactta cggttaacga ggcgcttatt ccagtagtca ttacacaggc gatactcttc
     8581 cgttttctcg cctgacttca tctggtcgaa atattcaccg ttaactgcca gttgcaggtt
     8641 agccacggtt aacctcctgc ggcggttctg gtagcggcat ccagtgggtt acctctttga
     8701 gatacaggtc ttcgccatca ccgtcatccc aagtgggatt gccatcatta aaccagtcgc
     8761 catatacgcc gacctgagtg ttggggatgt ttggtgggta gttgttttta aagtcagccg
     8821 ctaacacata gcattgtcgc tctcccattt ctggcattcg ctcactacag cttatccaac
     8881 catccggagt taccggagag ttgcccgata gctggttcaa cttgtaagtt tggcttacag
     8941 gttcggcacc atgaagcatg gcgtcgctcc gctctatgcc atccagcgcg attcgcagtg
     9001 cctgaattgt ggtggagcta tcgtttgggg ctattccata tcgctcgaat acagcgatat
     9061 gaccgcgcat aatctcaggc gtaagctctt tgtaagcata agcaagagac tctgatgtat
     9121 tatccggcac tactggcgat ggctgtttag cttctaaatc agcaattctg tcaaccacgg
     9181 catcgacggc atctgaaaaa ctgaaacagt tactccactc cggcctatcc ccggttgctg
     9241 caaaatacat atcagctaaa gcagactcag catggtcacg ctcgttaatg agctgctctt
     9301 cgcttttctc cagttcagca atacgcttac tcccatccga gataacacct tcataatatt
     9361 cacgctgctc gttgagtttt gattttgtct cttcaagctc agcaaccagt tttcgaagcc
     9421 cttcaatctc accattgcga gcaatgaggt cgcggcacat gctttcgttc tcatccagca
     9481 gtgcctgtac tacttcaggg ttgaaagctg cgatataacg agcgttgttc tctgcgtttt
     9541 tctgtccatc aaagccggtc catttgataa cgtcttcaca tcgtttatca ccgggcgtat
     9601 gcaccgcata cgtaccagta cccgtcgaaa taaatgcaac ccattctcct tgtgttgcct
     9661 gttttgctaa ctcacgcagt gcctgataat caatcttgct cactggctgc ctcctttgcg
     9721 aagctgggca gcaaagtcaa ctaaccactc agtcatttca accttcccta ccaggtctga
     9781 accagggtgc atacagcaat cactctgcgc cgctttgaaa tccttatact catattcttg
     9841 ggccaccaga ttttttgcag cttctatagc agcatccacc ccctgcgccc ggacttcagc
     9901 caggaaagca tcagtggttg gcgtttcagg tatctgtctc ctcatccgtt ctattgcatg
     9961 attgaacccg aagtcttccg cgagagatac gtcatccata ttgtcattgt catcctcaat
    10021 atcccgtgat tctggaattg cagactttat tcccgcattc tccgccgcca gcgccgcgca
    10081 cttggcctca aggttatcaa tcgtgattcc agcagaacga cactcccgca acgccgtttc
    10141 cagttttgat tcaagttcac cgaacttacg gacaagatat tcagcgtttg tttcgttaac
    10201 ctttaaatct cgtgggatgc atttaccttt cagaaatcca tccatctcaa ttagtgacat
    10261 ttgtttcatt tcttcccact ccgccacatc gcattcagat atttgttttg attcactgat
    10321 ggaaaagaat ttctcttaag caattcctct ctcgatggca ttggctttac gcgttggcga
    10381 ataatcattt ctgccggaag aatgccggga ttgtatgcaa gtcctctcat ggtaaattcc
    10441 tctttgttaa tttattcgta tgcctgctct ttcttcatcg agttttttta gcttgtatcg
    10501 catagctctt actgaataaa ttgagcggca ggttgcaatt gctatttctt ctgcggagaa
    10561 cttaccgaaa agtgatactt cggctcttgt ccagcgtctt ccacgaagtc ggctaacaat
    10621 gtcagcgcca atccttgttg ctttcgccat tactgctttt tcagtccttt ccagtttttc
    10681 tgcgataact tcaactggca ttgtcgccgc tacctcgcgc aagaaatcga cttcccattt
    10741 ctcccatgga gtctttttca taggcgatac cgttatttga taagaagtga aggtttccca
    10801 actttgagtt gagcacctgg gatatttatt cctgctttta gttggtgttt gattgccagt
    10861 ttgtcggctt taattgtcgt ttcgaactca acgtattcag gaggaatggc gcttgagtcg
    10921 atgatttcta cagtttctga cggtttgcgg attgttacct ggtgaatacc tgctctaatc
    10981 tttttcttgc caaccatttc aagcgatgac gctatatacg ccataatgct atcaatctta
    11041 ttttgaatta ctgctgctcg ttcatttagt gactttgcct cttccttgag gcgttcagca
    11101 taaccagatt catttttaat aatggcgaga agttgctcta ttttatcggt aaattctcct
    11161 tccatgcctt ctattgtgtc agcaatcatc tctggctcta aatctgaatc catcaatttt
    11221 gcgtattcat tggctatttc atatagtttg ctcactggca acctccagtt tcgctttgca
    11281 ttctatgtaa atggcttgta tgttctgctg caatttcatt ccagatgtca ggcgatatgc
    11341 ttctgcaaaa tatctcttca aatcatccat gttttcagcc tgagccattt catcacaaag
    11401 aagttgtgct ttatcagtta tttcctgctg gcgtttccgt tcatcttcgc ggatatcttc
    11461 ctctgatttg tgcggcataa ctggttcctg atgcatacct tcatcttcgt taagcaggtg
    11521 aatggcatta tccagtcgct gggctttagg ccagtatttg ctggcgcgtt taactatggt
    11581 tttacgcgcc atctcttccc agaatgtctt ccacggtcca ttctttgcct tgctcgttgc
    11641 ttccacagct ttaatttctg ccagactcat ttcttcagtc aggtagtcac catctgctgt
    11701 tttaaccgtg caataacctc caacaataga gcctcgctca ccaaatgcgt tgtatttgtg
    11761 ggttggtgct gaatcaaggc catttgattc ataggtgtcg tttgagtaca ccagtttgca
    11821 ttgcccccac ttaattgatc ctgtcgattg cgcaagatga agtaatccca tgtaactgat
    11881 atcaaggcac accatgccgt cgcgaggaac cagataagcc agtttgctgg ccgggtttaa
    11941 ggtgatgccg atcgccgcaa cattgatgat ggcgttctgt gcgctggttg gatttgccag
    12001 tgctgtttta gccaggtaat cgtttttctg gaaatactga attgcaaact ggctttcctt
    12061 agcccatgtc accgtctgtt cagtcaatgc tccgcagaat aactgctcct gctgtttaac
    12121 gaattcaacg atattgctca tgcagcttct ccataaatat gtctgcgttt gaatattgcg
    12181 aaggcatatt cagccttaac tctttcggtt attgcatccc agaaccattc agcggctttt
    12241 tcctgatagt tacagtcatc atcttccagc cagtcgatag cgtccttagt gtgttcatct
    12301 ggtttatata agcgaagcat ttcgcttatt gggtcgcaac gtttgcagag gcgatcaact
    12361 tcactgttga ttcgctcgta atcttcatca gtaaaacttg cgattatttg cgatatttca
    12421 cgcttatcat tcagagtcag aatcatcatc tttctcctgt tctttgtgct gattgagcat
    12481 tttgttcatc tgacgaatga attcttcgtc tgaccatgta tccgcaatgc tcattgctta
    12541 acgaccttaa tcagttgagt tacctcaatg atgtcctcgc ataaatcaga gtactgaaat
    12601 gggccgcagt tgtgagagtt agtgcgtcgg gcgaaagcca ttcttctaag ttctttcagg
    12661 gcttcttcca gttcttcgta agttggtttt ttcatggctg taccttttga ttcagaaact
    12721 caacaagacg atccagcaag ctcttaacgc gaggttgttt aaagtctgct ccggttaaaa
    12781 tgttttttcg tgaatgctgt accgataaaa tcgggttgaa agggcgaacc gatgccgccc
    12841 ctgcaatagc gaactgttgc ataggatgct ccttctgttt gattgcataa cgaaaacgcc
    12901 tcaagtgaag cgttattggt atgcatataa aaaggccctc acactggagg gcaaagaaga
    12961 tttccaataa tcagaacaag tcggctcctg tttggttacg agcgacattg ctccgtgtat
    13021 tcactcgttg gaatgaatac acagtgctta ttctcagcct acataatcat tccggttatt
    13081 attaccattt caatatcact atctttcata atgattggag ttgagttatc aactttactt
    13141 ttactccatt ttcctgcatc agtttttacc gctccacaat aagaaagcca atcatcatca
    13201 caatgttctg gatcactaat tctgacaaga acacgcgata gtgctttttc gatactttca
    13261 tcacaattag catttgtgta attgccgtga gacaatttca aatcgttaac ttcatcttca
    13321 ttggcatcaa aaatatagat ttcagtcgac tctggaacat tttcataaac cattaaaact
    13381 ttcattttta ccccatattg tttatgccaa aaataaaggc cgactatgcg gcctgaaatt
    13441 acttaaccaa tgatgctgca tattcgataa ggtaaagctt tggggccagc caaattttta
    13501 accaagtcat attggttact acaccaataa taaaaatccc ccacagagtc aaaactccaa
    13561 ccaatggcat gataagaagg ttaatatctc ctttgctatc ccaaaccatt gtcggcctgt
    13621 atttgggatt tcccctctcc catgagtatc cttcatcacc gattttacct gtctcaactc
    13681 tttggcactg cttcttcata aaccagaaaa ccagtgggat tgttagaatg gctattaatg
    13741 ttttaatcag actgtcaacc atattccata gcagcaactg atgaacaaca tcaggaatct
    13801 gcgcttggct gaatgaaaca gccgcgtcta ttccattgct ggctttttgc agtagttcta
    13861 cgagaatctt gtttgcttgt tcttccatat ctcacctcaa tcgtaataag ctggaattga
    13921 ttttccgcgt tgcttctggc ggcctgaaca ggtcacaccc atttcactgc gtggcttgct
    13981 gtaccatgtg cgctgattct tgcgttcaat acgttgcagg ttgctttcaa tctgttcgtg
    14041 gtattcagcc agcactgtaa ggtctatcgg attcagtgcg ctttctactc gtgatttcgg
    14101 tttgcgattc agcgagagaa ttgggcggtt aactggtttt gcacttaccc caaccaacag
    14161 gggatttgct gctttccatt gagcctgttt ctctgcgcga cgttcgcggc ggcgtgcttg
    14221 tgcatccatc tggattctcc tgtcagttag ctttggtggt gtggtaggtg ggagacccat
    14281 ttcgacctgc ttcggccgac ttcaattcgg caatagttcc gcaggcctcg ccgctttacg
    14341 tgcgacatat tcccatccat gaacccttca ccacacccca aagctttctg ctttgaatgc
    14401 tgcccttctt cagggctaaa tttttaagag cctcaccttc aatggtggtt agtgcgtcct
    14461 gctgatggct aaatagtacg atttgta
//
LOCUS       DAZKGF010000126        14277 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZKGF010000126 DAZKGF010000000
VERSION     DAZKGF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.126"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            289..1494
                     /gene="gltS"
                     /locus_tag="ACHOQV_003613"
     CDS             289..1494
                     /gene="gltS"
                     /locus_tag="ACHOQV_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFP9631915.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(1528..3609)
                     /gene="recG"
                     /locus_tag="ACHOQV_003614"
     CDS             complement(1528..3609)
                     /gene="recG"
                     /locus_tag="ACHOQV_003614"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP9631916.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDAPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(3615..4304)
                     /gene="trmH"
                     /locus_tag="ACHOQV_003615"
     CDS             complement(3615..4304)
                     /gene="trmH"
                     /locus_tag="ACHOQV_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFP9631917.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(4311..6419)
                     /gene="spoT"
                     /locus_tag="ACHOQV_003616"
     CDS             complement(4311..6419)
                     /gene="spoT"
                     /locus_tag="ACHOQV_003616"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP9631918.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(6438..6713)
                     /gene="rpoZ"
                     /locus_tag="ACHOQV_003617"
     CDS             complement(6438..6713)
                     /gene="rpoZ"
                     /locus_tag="ACHOQV_003617"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9631919.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(6768..7391)
                     /gene="gmk"
                     /locus_tag="ACHOQV_003618"
     CDS             complement(6768..7391)
                     /gene="gmk"
                     /locus_tag="ACHOQV_003618"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9631920.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPSEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            7649..9334
                     /gene="ligB"
                     /locus_tag="ACHOQV_003619"
     CDS             7649..9334
                     /gene="ligB"
                     /locus_tag="ACHOQV_003619"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFP9631921.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGIYDQLSARLTQWQRCFGEETHRDAMMPPLNGAVIHPVAHTGVR
                     KMADKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVR
                     LISAVPQTVSGPLANSTLQGEIFLKREGHIQKQMGGINARTKVAGLMMRQDDSDTLNS
                     LGVFVWAWPDGPQLMTDRLKELATAGFTLTQTYTRAVKNADEVARVRNAWWKTKLPFV
                     TDGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVARVAEVKAIQFAVGKSGKISVVASL
                     APVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPT
                     PPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRSLHQTHRFEHIF
                     SWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDER
                     SWSQLLLSTEQFWQQLPGTGSGRVRQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(9331..9948)
                     /gene="yicG"
                     /locus_tag="ACHOQV_003620"
     CDS             complement(9331..9948)
                     /gene="yicG"
                     /locus_tag="ACHOQV_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFP9631922.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10240..11064)
                     /gene="dinD"
                     /locus_tag="ACHOQV_003621"
     CDS             complement(10240..11064)
                     /gene="dinD"
                     /locus_tag="ACHOQV_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFP9631923.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACLNSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVNIKQLENSVKITEKK"
     gene            complement(11471..11552)
                     /locus_tag="ACHOQV_003622"
     tRNA            complement(11471..11552)
                     /locus_tag="ACHOQV_003622"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11512..11514),aa:Tyr,seq:gta)
     gene            complement(11935..12342)
                     /locus_tag="ACHOQV_003623"
     CDS             complement(11935..12342)
                     /locus_tag="ACHOQV_003623"
                     /inference="COORDINATES: protein motif:HMM:NF020345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFP9631924.1"
                     /translation="MNEQTHHVPKDIERLKFMNDIDGGDKPGDAWCDSLADIVSHSVA
                     ELTRNGMEEADAFRIAKRVVAAIAFYAGGRSLYLPTGRSLFAALREYEIYYRWDNGEK
                     LQALRKEYAISETHIYDIIRRLRKKHANKITQD"
     gene            complement(12432..12728)
                     /locus_tag="ACHOQV_003624"
     CDS             complement(12432..12728)
                     /locus_tag="ACHOQV_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9631925.1"
                     /translation="MVHDRIAEELESKGFYRRAAARWAEVMMLVETDRERERVAMRRA
                     ECYGKAQRPPEPPEENYADLRKAVNRTYAEMGIGGRCKKSGVATRNKHRAGTVG"
     gene            complement(12740..12985)
                     /locus_tag="ACHOQV_003625"
                     /pseudo
     CDS             complement(12740..12985)
                     /locus_tag="ACHOQV_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(13411..13701)
                     /locus_tag="ACHOQV_003626"
     CDS             complement(13411..13701)
                     /locus_tag="ACHOQV_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631926.1"
                     /translation="MHKLFSLPDALCEFIRALPAPDLQEAHQSETWPEDNTTSNGQTL
                     ADMCAPSAAWLAARDAYISHVMTCPHCATSGVKKPRHCAEGERLRQNYDHTR"
     gene            complement(14000..>14277)
                     /locus_tag="ACHOQV_003627"
     CDS             complement(14000..>14277)
                     /locus_tag="ACHOQV_003627"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631927.1"
                     /translation="FEWELCGNWWEPQRTKNNRHGSHKKPPWELVGTTESRVVLGGSH
                     SSHQSHQLAQYYARQKTHHKNPEIPYVALDARQFQCAKRVKQQNHTE"
BASE COUNT         3101 a         3609 c         3808 g         3759 t
ORIGIN      
        1 aaacagacat cgaaaacaat cccgttgtag caaagcgggc attttagctg accgaaagag
       61 aaaagcaaac gtttgccacg gcctctgcta aatatttaaa tgagataaat gcactttttt
      121 atataacttt tggtattttt ctgcttaaaa tccatgccat ttgttcatta acagctgaga
      181 atttgaccca ggaaagcata actcgatacc cgtcatagtt cataagttgc cactgcgcga
      241 tgaagtatga cgagtatgaa agagtgatgc ggatacaaag gagtaactat gtttcatctc
      301 gatactttag caacgcttgt tgccgcaacg ctgacgttgc tgctcgggcg taagttggtc
      361 cattccgtct cctttttgaa gaaatacacc ataccggaac ctgttgcggg tggtttgttg
      421 gtggcgctgg cgctactggt actgaaaaaa agcatgggct gggaagtcaa ctttgatatg
      481 tccctgcgcg atccgttaat gctggcattc ttcgccacca ttggcctgaa cgccaacatt
      541 gccagtttgc gtgccggtgg gcgtgtggtt ggcatcttct tgattgtggt tgttggtctg
      601 ttggtgatgc aaaatgccat tggcattggt atggctagcc tgttagggct tgatccgctg
      661 atggggctgt tggccggttc tattacgctt tccggcggtc acggtacggg cgctgcgtgg
      721 agtaaattgt tcattgaacg ctatggcttc accaatgcga cggaagtggc gatggcctgt
      781 gcgacgtttg gtctggtgct gggcggcttg attggcggtc cggtagcgcg ctatctggtg
      841 aaacactcca ccacgccgaa cggtattccg gatgaccagg aagtcccgac ggcgttcgaa
      901 aagccggatg tgggccgcat gatcacctcg ctggtgctga ttgaaactat cgcgctgatt
      961 gctatctgcc tgacggtggg gaaaattgtt gcgcaacttt tggctggcac tgcttttgaa
     1021 ctgccgacct tcgtctgtgt actgtttgtt ggcgtgattc tgagcaacgg tctgtcaatg
     1081 atgggctttt accgcgtctt tgagcgtgcg gtatccgtgc tgggtaacgt aagcctgtcg
     1141 ttgttcctgg cgatggcgct gatggggctg aaactatggg agctggcttc gctggcgctg
     1201 ccgatgctgg cgattctggt ggtacagacc atcttcatgg cgttgtatgc catcttcgtt
     1261 acctggcgca tgatgggcaa aaactacgat gcggcagtgc tggctgcggg tcactgtggt
     1321 tttggcctcg gtgcaacgcc aacagcaatc gccaacatgc aggcgatcac tgaacgtttt
     1381 ggcccgtcgc acatggcctt cctggtagtg ccgatggtcg gtgcgttctt tattgatatc
     1441 gttaacgcgc tggtgattaa gctgtattta atgttgccga tttttgccgg ttaaccgatg
     1501 aagcggcggt cttctcaccg ccgcctttta cgcattcgag taacgttccg tttctggcat
     1561 ccagcgttct atcagggctt ttgcctgctg tgggtaacgt tcgtgaatat ggcgtgccag
     1621 gcgctgaact tccgggatca tcgcctgatc gcgcagtaaa tccgccactt taaattcagc
     1681 attacccgtc tgacgcgtgc ctaacaattc gccagggccg cgaatctcca gatctttttg
     1741 cgcaatcaca aacccgtcgt tgctgtcgcg cagtacttgc agacgaattt gcgccgtttt
     1801 agagagcggc gttttgtaga gcagcacgca gtgagaagcc accgcaccac gtcccacgcg
     1861 cccgcgaagc tggtgcaact gcgccagacc cagacgctcc gggttttcga taatcatcag
     1921 gctggcgtta ggcacatcaa cgccgacttc aataacggta gtggcaacca gcaggtgtaa
     1981 ctcgccttgt ttaaatgatg ccatgacggc ctgcttctcg gctggtttca tccgtccgtg
     2041 taccagacca acattcaact ctggtagcgc cagtttcaac tcttcccagg tagcttccgc
     2101 cgcctgcgct tccagtaact ctgactcttc aatcaacgta caaacccagt atgcctgacg
     2161 accttcagtt atgcaggcgt ggcgcacgcg gtcaataatg tcggtacggc gggtatcagg
     2221 aatagcgacc gtagtcactg gcgtgcggcc tggcggcagc tcatctatca ccgaggtatc
     2281 gagatcagca tacgcagtca ttgccagcgt tcgggggatc ggcgtggcgg tcatgatcaa
     2341 ctgatgagga tggaagcctt gctgctggcc tttctcccat aacgccagac gctgatgcac
     2401 gccaaaacga tgctgttcgt cgataatcac cagcgccagg ccgttaaact gcacctgttc
     2461 ctggaagatg gcgtgtgttc cgacaatcat ctgcacctga ccgctggcga tggcttcctg
     2521 ctgtgccagt cgcgctttac ctttctgctt accggcgagc cagcccactt cgataccgag
     2581 cggtgcaaac cagttgcgga agttattcgc gtgctgttcg gcgagtaatt cggttggtgc
     2641 catcaatgcc acctgtttgc cgtgcgcaat cgcacgcagc gcggcgaggg cagcgaccag
     2701 cgttttaccg gaacctacat cgccctgtac caggcgcatc atcggcgcat ccagcgccat
     2761 atcgcgctcg atctccgcca ccacgcgtgc ctgtgcgccc gttggcttga acggtaaggc
     2821 tgcgaggagt ttatttttca gcgcgtcatt ggcgctaagc ggctgggcat ggaaacgttg
     2881 tgcaccagcg cgtaacgcca acatgctgag gttgtgcgca agcagttctt ccagaatcag
     2941 acggcgttgt gctggatgct gcccggtttc gagatcgcta agctgtagcg tcggcggcgg
     3001 gcggtgcaaa gtgcgcaacg cttccggtag cgtcatcatt ccttgtgaca gttccggcgg
     3061 caggagttct tcaatggcgc aggtgtcgag cagatccagc gcctgatcgg ttaatttacg
     3121 cagcgtggcc tgctttacgc cttccgttgt tgggtaaacc ggcgttagcg tttcctgcaa
     3181 ctctggcgta ctgagatcgc cctgcacgcg gtattccggg tggatcatct ccgcaccata
     3241 tttaccgcgc tttgcttcgc cataggccag cacgcggcgg cctgtcgcca ggctattttt
     3301 cattgccgcg ttgaagttga aaaagcgcat ggtgagaatg ccggaaccgt cgctgatctg
     3361 gcaggtcatc atccgccgac cgccgaagga gatattgcag ttcagcactt cgccttccac
     3421 cgtggcataa acgcccggta gtagttctcc gatggggtag agatgggtgc gatcttcgta
     3481 gcgcagggga aggtgtaaga gtagatcctg cacggtatgc aggttgattt tcgccagctt
     3541 gttactaagc gctgcgccaa cgcccgttag ggaactgagc gggacagcat ctaacaggcg
     3601 acctttcatg gcacttaccc tgcagcctgc atggtagccc accagtcagc atcggcttca
     3661 atttcgcctt gctgattaac gtgggggtaa ggcaggcctt tgcgttttgc gactttcgcc
     3721 agcaccggat agccgccttc aaacagcagg cgttgttgct ctgcttccgg caacatgctg
     3781 ttttcacgca ggtacatgcc tgcattttgc cgctggcgct gggcttcata aagaatgagg
     3841 gctgaggcaa cggaaacatt cagcgactgc accatgccga tcatcggtat gatgatgtcc
     3901 cgatccgcca gggccaacgc ttcctgcgtg atgcctgttt tctcctgtcc catcaaaata
     3961 caggtcgggc gagtgtaatc aatttcgcgg aaatcgacag cgttatcaga aagatgggtt
     4021 gccagaatct gcatgccctg gcctttcaga tgagcgacgg catcgccaat ggtgcggtgc
     4081 gttttcacct gtacccagct gttactaccc gcagctgccg aagccatggt gcgcatccgg
     4141 ctaccaggcc agacagcatg aacttcatgt acaccaacgg catctgcggt acgaataatc
     4201 gcagaaacgt tatgaggttt gtggacctgc tccatgcaga cggtcagatc aggctgccgc
     4261 ctggcgagca tttcgcagat gcgtgcataa cgtgttgggt tcataaaaca ttaatttcgg
     4321 tttcgggtga ctttaatcac gtctggcatc acgcggattt tgcgcatgat attcgccaga
     4381 tgcacacggt cacgggcggt cagacgaata aaggcgctgt agacgcgacc atctttctct
     4441 tccgtattca aactttgaat attcgaggtg gtggtgttaa ttgccgctgt caggtttgcc
     4501 agcgcaccct gatgattgaa catctccacc ttgatttcgg tgatgaactc ctgcgccgtc
     4561 tctttatccc attccacagc cataaacttc tctggctctt tctggtagcc acggatgtta
     4621 cggcaggatt catggtggat caccagacct ttaccggggc tgacgtgggc gataatcggg
     4681 tcgccaggaa tagggcggca gcatttcgca aaggtgatca gcacgccatc ggcaccttta
     4741 atgggcagat gtccgtgact ttgggttgcc ggtggaatgg aggcgtcccc atgttgcaga
     4801 tttttcgcga ccaccacgct cattgcgtta ccaaggccga tttcagccag cagatcgtca
     4861 agcgttgcca gcttcatgcg gtccagctcg cgctgaatat tttcctgcgg gatttcattg
     4921 agcttgcggc tgccacccaa cgcatggttg agcagacgac ggcccaggct tacagaatca
     4981 tcacgcttga ggtttttcag caattgacga attttggcgc gcgctttcga gctaacgaca
     5041 aagttcagcc acgcggcatt cgggcgagcg cccggtgcgg taataatttc aacggtttga
     5101 ccgctggtaa gcggctgcga cagcgggtaa ggctggcggt cgacgcgtgc gcccacgcag
     5161 gcatgaccga tatcggtatg caccgcataa gcgaagtcga cgggcgttgc accggcaggc
     5221 agttcgacaa tgcgcccttc tggtgtgaaa acgtaaatct catccgggaa gagatcggat
     5281 ttaacgctct cgataaattc aaacgagcta ccggcactct gttgcagctc cagcaggctt
     5341 tgcatccagc gctgggcgcg tatttgcgcg gtagtgctgg tttcgccgtg ctctttataa
     5401 gcccagtgcg cggcaacacc catctccgcc atctgatcca tatcttcggt acggatctgg
     5461 acctcaaccg gcacgccgtg cgggccaatc atcgaggtgt gcaaagactg atagccgttc
     5521 gcttttggaa tggcgatata gtctttcacg cggcccggac gcggcttgta caggctgtgc
     5581 atctggccca gcacgcgata acaggtgtca gaatcattga cgatcacgcg gaaagcgtag
     5641 atgtccatga ttgagtgaaa acgctgctct ttgagcacca ttttgcagta aatcgaataa
     5701 agatgctttt cgcgaccact gacgcggcac ggtattcccg cttcctgcaa acgcccttcg
     5761 atttcagaaa gaatcttctg gatcatctct ttacggttgc cgcgcgcggc tttcaccact
     5821 tctttgatta cgcgataacg gttgggatac agcgcctcaa aacccagctc ttcgagttcg
     5881 gttttaatgt ggtggatacc taaacggtgc gccagcgggc tgtaaatttc gagggtttca
     5941 cgggcgatgc ggcgacgttt gtccgggcga agtgagccca gcgtgcgcat gttgtgggta
     6001 cggtcggcaa gtttgatgag aatgacgcgg atatcctgca ccatcgccat aatcatcttg
     6061 cgaaagtttt cggcctgcgc ctctttctta tcgcggaact tgagcttatc aagtttcgac
     6121 accccctcta ccagctcggc gacgctttta ccaaaaagct gttccatgtc ctggtaggtg
     6181 gcgggagtat cttcaatcac gtcatgcagc agcgccgcca taagcgtttc atagtcgagt
     6241 ttcatctcgg ccagaatgca ggcaaccgcc accgggtgcg tgatataggg ttcaccgctt
     6301 gaacgtgttt gcccctcgtg agcatcacgt gcaacgagat acgcctgccg cagacgcttg
     6361 atttggtctt ccggcaggta ggtttgaatc agttgattca ggctttcaaa cagatacaag
     6421 ggcgacccgc tttgtgatta acgacgacct tcagcaatag cggtaacggc ttgtaattca
     6481 gcggcttcct gctcttgctg ttcctggcgt tcgcgaacgt cgaggatctg gttgttgatc
     6541 agaccttctt cgatttcgcg cagcgcgatt acagtggttt tatcgttttc ttccggtacc
     6601 agcggatcct ttccgcctac ctgcatctga cgagcgcgac gcgcggcgac cagtaccagg
     6661 tcaaaacggt taccaatttt ctctacagcg tcctgaacag ttacgcgtgc catacttaaa
     6721 aagctccaca ggtgaagaaa tgactgggca tgatactgaa atcaggttca gtctgccaac
     6781 aatttgctga ttaaagcgtc atgacgctgc ttttggcggc tcatgcgcag acgttcggcg
     6841 cgaataatgg tcttcaaatc ggtcaacgcg gtatcgaagt catcattcac aatcaggtaa
     6901 tcatattcgg cgtaatggct catttctgca acagcttgcg ccatacgctt tgcaatgacc
     6961 tcttcgctgt cctgaccgcg accgcgtaga cggcggtcca gttcaatttt ggacggcggt
     7021 aaaataaaga tactccgcgc gtgcggcatc ttctggcgaa tttgctgcgc accctgccag
     7081 tcgatatcga gaaaaacatc gacaccggtc gccagtactt gctcaatggc ctcacgcgaa
     7141 gtgccatagt aattaccaaa aacttctgcg tgttcgagga acgcatctct gctaatcatt
     7201 tctttaaatt catcatgatt aacaaagaaa taatgttcac cgtggacctc actaggacgc
     7261 ggttggcgtg tggtgtgtga aacagaaacc tgggtgtcat acaacggttg ggtttttaat
     7321 aaagcctgaa tcaggctgga tttacccgcg ccactggggg cagaaacaat ataaagcgtg
     7381 ccttgagcca tgagtatctt tcgtatgtga ttagcgaaat aaagcctaca tacgagctta
     7441 ttatacacgg cgcggccatg tgacgtagcc cttgctcaca ctttttgcgc aaagttgttg
     7501 cgttttgcgt agctgcttcc gcatttacct gtcattcact gcaataaaga cgattctcag
     7561 gaagcgtcct cgcagactcc gtttttgcca ctcggctttg ctttcatcac gttgtatatc
     7621 tgcctgacta tctacagcgg aggtgatgat gaaagtatgg atggcgatat taataagtat
     7681 cttgtgctgg caatcatcgg cgtgggcagt ctgtccggcc tggtcgccag ccagagcaca
     7741 ggaagaaatt tcccgcctgc aacagcaaat aaaacagtgg gacgatgact actggaaaga
     7801 agggaaaagt gaggtggaag acggtattta cgatcagtta agtgcccgtc tcacgcagtg
     7861 gcaacgctgt tttggtgaag agacgcatcg cgacgctatg atgccgcctt taaacggtgc
     7921 ggttatacat ccggttgcac atacgggtgt acgcaaaatg gcggataaaa tcgcattaag
     7981 tctgtggatg cgcgaacgta gcgatctttg ggtgcagcca aaagttgatg gcgttgcggt
     8041 aaccctggtt tatcgggacg ggaaactgaa caaagcaatc agtcgcggca atggcctgaa
     8101 aggcgaggac tggacgcaga aagttcgctt aatttccgct gtgccgcaaa ccgttagcgg
     8161 gcctttagcc aacagtacgc ttcaggggga aatatttctc aaacgcgagg ggcatatcca
     8221 aaaacaaatg gggggaataa atgcccgcac aaaagttgct ggtctgatga tgcgccagga
     8281 cgatagcgac acgctgaatt ctctgggcgt ttttgtctgg gcatggccgg atggaccgca
     8341 gttaatgacc gatcgtttaa aagagctggc taccgctggt tttactctaa cgcagacgta
     8401 tacccgtgcg gtgaaaaatg ctgatgaagt tgcgcgcgta cgcaatgcgt ggtggaaaac
     8461 gaaattaccc ttcgtcaccg atggcgtggt tgtgcgagcg gcgaaagaac ccgaatcccg
     8521 ccattggcta ccaggccagg cagaatggct ggtggcctgg aaatatcaac ctgtagctcg
     8581 ggttgccgaa gtgaaggcga ttcagtttgc ggtgggtaag agcggtaaaa tatcggtggt
     8641 tgcgtcactc gcacctgtca tgcttgatga taaaaaagtc cagcgggtga atattggttc
     8701 cgtcaggcgc tggcaggagt gggatattgc gcctggtgat cagatcctcg tcagccttgc
     8761 cggtcagggt attcctcgca ttgatgatgt ggtgtggcgc ggtgcagaac gtacaaaacc
     8821 gacaccgcca gaaaaccgct ttaactcgtt gacctgctac tttgcttctg atgtttgtca
     8881 ggaacagttc atttcacgct tagtctggct ggggtcaaaa caggttcttg ggctggatgg
     8941 cattggtgag gccggctggc gctcgctgca tcagactcat cgctttgagc atatcttttc
     9001 ctggctttta ttaacgccag agcaattaca gaacacgccg gggatcgcga aaagtaaaag
     9061 tgcgcagcta tggcatcagt ttaatctggc tcgtaagcag ccttttactc gctgggtgat
     9121 ggcaatggga ataccgctaa cccgggcggc gcttaatgcc agtgatgaac ggtcctggtc
     9181 gcaactttta ttgagcacgg agcagttctg gcagcagctg ccggggactg gatcggggcg
     9241 tgtcagacag gttattgaat ggaaggaaaa tgcgcaaatc aagaagctgg gcagttggct
     9301 ggctgcccag cagatcacag gttttgaacc ttagtgtcct tcgtggctgt agtaaaagac
     9361 cggtaatccc agcttaaggc gtagcgccag taagcgggca aaaaagccga ataccagggt
     9421 ggagataatt accacatcat ggttagaaac atagtgttgc agcgcgatgt acagcacggc
     9481 ggaggcaaat gagacaccgg catacaactc tttctggaat accagcggga tgcgtttaca
     9541 gaacatatcg cgcaaaacgc cgccaaatac gccggtcgtc acagctgcga caacggcaat
     9601 gatcgggccg tgtcccatat ccagtgcaac ctgtgcgcca atgatagaaa agaccaccag
     9661 tccgagcgca tcgagcacca gaaacacttt gcgcaggtaa ggcattacag gagcaacgat
     9721 agtggtgagt actgcggcgg tagcgacaat aatcacatat tccgggtgtt tgacccagcc
     9781 gagcggatag tggcccagca gaatatcgcg cactgaccct ccgccaattg ccgtggcggt
     9841 cgcaataata attacgccaa atgtatccat gcgccgtcgt ccggccgcca gcgcccccgt
     9901 catggcttcg gcagtaatgc caaccaaata caaaatgtgt aacagcatgg ttctctccct
     9961 aaaaatctgt ctggcagggt agcgatttgt gcgtattgtc acgattgaga ttttttaagg
    10021 tgagaggaaa ttgataactt gtagttatgt gaaatcgcta ttttctgtag cgtagatgca
    10081 ttcttctgac ttcttgtctg agaaaatgca taagaaaaaa taggttaaaa ttgaggggtg
    10141 gaaaagaaag gtgcaatgtt tagccattgt gttcaccgca cctggacttt ggcttacgca
    10201 tcgggtatct caatcaacaa aaagagtttt tagtttctgc tatttcttct ctgtaatttt
    10261 tacactgttt tccaactgct tgatgttgac ctgcggggtc ggcaactcct caggcatggt
    10321 tccgccaagt tcctgaatgg tttgcctcac cttgcgaccc acgtcaaagt gtgtggtgtt
    10381 agcttgctgt tttgaattca cgccatcccg cttgagtttt tcttctgttt gggtagctcg
    10441 aaagagatta gccgccagtt ctgttgaacc catatgatcc aggatcttct gactcttttt
    10501 cagccccttc cgctgatgga tggctttctg atctaatcca ccatacagcc cccggtaacc
    10561 atgattctga aatatggcga aatcgatagc tgtcgctacc ccagcttgct gcgctgcctc
    10621 aactaattgt ttgttatgtt ctttcaactc atttctcaga aaaagacgct tttcgtcttc
    10681 acgaagttgc ctgaaagctt catcgtcagc cagctcctgc cgtcgggtct ggatagcaaa
    10741 ataagtttgc cctgccgcaa taaccggttt cgcagggtcg ccgttttgta ctaccaggta
    10801 acaggcatag cgggagagta cgatgtcttt taatgctctt ttggcatttg agccaatttt
    10861 gaccatatcg aggatctcct cgaaatggtc atcaattgtg tgaccactat tcagacaagc
    10921 ttctttggcg cgcgttaata caggtataaa gtgacgatac tctgaataac ccagtagttt
    10981 tccaagttgc cttgctgacc attgttctgc tccgtttgca ttaatcagct ttatctcttc
    11041 aaaaggttga tgatgttcgt tcattgccca ctccatgtga tatcgattgc aaggaaagta
    11101 agtctacaac tgtgtttata tacagttgta agtatctcac gttctcactg cgctgggcaa
    11161 aaaaacatcg caaaatctgg agcaggcacg caaaacagaa ttgaatgttg cggggaggaa
    11221 aaatgccgct ctgtttaagt gggcggcatt tgtgaaggct gaaaaggaag aattggcgtt
    11281 acgagttgga tctgaatgtt tagtatgtgc gttaattgct tttattatgc ggttctttgg
    11341 gttgggttaa tttgtatatg tacacgcgta tgtacacatt tttcgtgagt gctcaataaa
    11401 acgggatacg aaagccgttt ttatgtggct ttaaagtggg tttagaaatg gcgcaattgt
    11461 gcttttggcg tgctggtggc gggggtcgaa cccgcataca tgatcagcct gtacaggctg
    11521 gcaccgactc tacccgttga gcgtacacca gcaaaaaagc gatgaggttg ttggctgctg
    11581 gtggtggggg tcgaacccac ataaacatat ggcccgttag ggtttatgcc gctttaccga
    11641 ttaagagtac accatacagc caggcaaaat agtaattatg cgttatgcgc gggtaaagtc
    11701 cgttttgtct ggtagcacca gatgaacggg tatcattatt tcacctgatt tcacgtgatt
    11761 tcgcttaatt tcacaaaaat caccgtcttt caccttcttt cacaaaaaac aaattatcat
    11821 taaagcgcct ttcgctctct gatgcgtttt ggtgttttgt ggtgtctgtg atgccctgaa
    11881 aggggatttt tgcgatagcg gcgcaaatat aacggttgct gctggtatct gtggttagtc
    11941 ttgcgtgatt ttattggcgt gcttctttct aagccgccga atgatatcgt aaatatgggt
    12001 ctcggatatc gcgtactctt tacgtaatgc ctgcaatttt tcgccgttat cccacctgta
    12061 gtaaatctcg tattcgcgca atgctgcgaa aagtgatctg cctgttggaa ggtaaagact
    12121 ccgcccacca gcatagaagg cgattgcagc aacaacccgc tttgcgattc tgaaagcgtc
    12181 tgcctcctcc atgccattac gggtaagttc tgccactgaa tgcgacacga tatcagcaag
    12241 cgaatcacac catgcatcac ctggtttatc tccgccgtct atgtcattca tgaatttaag
    12301 ccgctcgatg tcttttggta cgtgatgggt ttgctcgttc atccggtagc ctcttgtgtt
    12361 ggtgtatctg gtcctaccag tttaatttaa cgtcgcgata aaacgatata cacgcacaca
    12421 tatcagaaag gctaaccgac tgttccggct ctgtgcttat ttctggtagc tacgccagat
    12481 tttttacatc tccctcctat ccccatttct gcgtaagtgc ggtttactgc tttcctcagg
    12541 tctgcgtagt tctcctctgg cggctccggt ggcctctgtg ccttcccgta acactctgcc
    12601 ctgcgcattg ccacccgttc gcgttccctg tcagtctcca ccagcatcat cacttcggcc
    12661 catcgcgccg ccgctctccg gtaaaagcct ttcgactcca gttcttccgc tatgcggtca
    12721 tgtaccatcg ttcaccccct cagaacggaa tattgtcacc gcaggggtca taacttcccg
    12781 ctggtggctg attgctctgt gtgcctgtgg tactgcgtct gctcccgcct ggacgcgtcg
    12841 cccgtgcact gattatgctg tccgctatga cctgataacc ctgccgcgtt tccccgttct
    12901 gtccggtcca ctggcttacc tgcatcgtgc cggatacgct gattacgttg cctttctcat
    12961 atcctgccag tcggtcggcc tgtctgaaaa acacgctttc ttaaacaatg cctaaaagcc
    13021 gattttatgg tgctttgaaa cggtttttaa aatggcgctc gtggtgtact ctctggcatt
    13081 caatggcgct ctgtgatgtg gctggtggta tgtggccgcc atgcataatg gggctgattt
    13141 tgttcatggc ccggcaatgg gtgagcattt tttaccatat tcagggtaac aatgggcgaa
    13201 aaagttcatt tctggcatgg cctgaaattc catgcagccc gcgccgcgtg tgctcttgcc
    13261 ggggaaaacg tcgcggaata atgaccgcac cccaccttat ggcggtgcag tcccacgaaa
    13321 tcccggttat ttccgtttat tcccggatta atcccgcctt ttcccgcatt ctcccggctg
    13381 ttcccggaat taatcgccgg ggtgtggtgg ttatctggtg tggtcgtagt tctgccgtag
    13441 tcgttcaccc tcagcgcaat gacgcggttt tttaacgccg ctggtagcac aatgcgggca
    13501 ggtcatgacg tggctgatat acgcatcacg ggcagccagc cacgccgcag acggggcgca
    13561 catatccgcc agcgtctggc cgttgctggt ggtgttgtct tccggccatg tttcggactg
    13621 gtgcgcttcc tgtaaatccg gtgcaggtaa tgcccggata aactcacaca atgcatccgg
    13681 caggctgaat aacttatgca taaatctgat gttctgaatc gctgaaaacg tgcgttttat
    13741 ggtgtttttt cgtgatttct gcgtgttctg atgtgctcat ttcgctgtat cccttgtgtg
    13801 gcgcgggctg cgcgtgtgtt tcggtgttct tttttgccgc cagaaaacgg taatcaggca
    13861 tataaacacg gctgtaagcc gcgccgcgtc tggcttcgcg tgattttgtg cgctaaaaag
    13921 tgcatttttg cccttaaaaa gcgttattcg tgattcactg gtaaatgctc cacatgccgc
    13981 gccattgctg ggcttttcgc tattctgtgt ggttttgctg tttaacccgt tttgcacact
    14041 gaaattgcct tgcatccagc gccacgtaag ggatttccgg gtttttatgg tgcgtttttt
    14101 gccgtgcgta atattgtgca agttggtggg actggtggga actgtgggaa ccgcctagaa
    14161 ccacgcggct ttcagtggtt cccaccagtt cccacggtgg ctttttgtgg gaaccgtggc
    14221 ggttattttt tgttctctgt ggttcccacc agttcccgca gagttcccac tcaaaaa
//
LOCUS       DAZKGF010000127        14215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZKGF010000127 DAZKGF010000000
VERSION     DAZKGF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.127"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(7..195)
                     /gene="bcsR"
                     /locus_tag="ACHOQV_003628"
     CDS             complement(7..195)
                     /gene="bcsR"
                     /locus_tag="ACHOQV_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFP9631928.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            468..2039
                     /gene="bcsE"
                     /locus_tag="ACHOQV_003629"
     CDS             468..2039
                     /gene="bcsE"
                     /locus_tag="ACHOQV_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFP9631929.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            2036..2227
                     /gene="bcsF"
                     /locus_tag="ACHOQV_003630"
     CDS             2036..2227
                     /gene="bcsF"
                     /locus_tag="ACHOQV_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFP9631930.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            2224..3903
                     /gene="bcsG"
                     /locus_tag="ACHOQV_003631"
     CDS             2224..3903
                     /gene="bcsG"
                     /locus_tag="ACHOQV_003631"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFP9631931.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDITEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFSEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(3990..4097)
                     /locus_tag="ACHOQV_003632"
     CDS             complement(3990..4097)
                     /locus_tag="ACHOQV_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9631932.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(4473..4580)
                     /locus_tag="ACHOQV_003633"
     CDS             complement(4473..4580)
                     /locus_tag="ACHOQV_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9631933.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(4956..5063)
                     /locus_tag="ACHOQV_003634"
     CDS             complement(4956..5063)
                     /locus_tag="ACHOQV_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9631934.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(5439..5546)
                     /locus_tag="ACHOQV_003635"
     CDS             complement(5439..5546)
                     /locus_tag="ACHOQV_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9631935.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASLIVNWLNKRK"
     gene            6022..7293
                     /locus_tag="ACHOQV_003636"
     CDS             6022..7293
                     /locus_tag="ACHOQV_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFP9631936.1"
                     /translation="MQHNTLPKHDQKMPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(7323..8327)
                     /gene="dppF"
                     /locus_tag="ACHOQV_003637"
     CDS             complement(7323..8327)
                     /gene="dppF"
                     /locus_tag="ACHOQV_003637"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFP9631937.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(8324..9307)
                     /gene="dppD"
                     /locus_tag="ACHOQV_003638"
     CDS             complement(8324..9307)
                     /gene="dppD"
                     /locus_tag="ACHOQV_003638"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFP9631938.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(9318..10220)
                     /gene="dppC"
                     /locus_tag="ACHOQV_003639"
     CDS             complement(9318..10220)
                     /gene="dppC"
                     /locus_tag="ACHOQV_003639"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFP9631939.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(10230..11249)
                     /gene="dppB"
                     /locus_tag="ACHOQV_003640"
     CDS             complement(10230..11249)
                     /gene="dppB"
                     /locus_tag="ACHOQV_003640"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFP9631940.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRLRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11267..11494
                     /locus_tag="ACHOQV_003641"
     CDS             11267..11494
                     /locus_tag="ACHOQV_003641"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631941.1"
                     /translation="MTRSLELRVCIAHKSIPALVEQGMKYCCRMALHAEAQRHRVCRL
                     LTKSALFMPDAAQTPYPTYKIVQTQYIAEFS"
     gene            complement(11557..13164)
                     /gene="dppA"
                     /locus_tag="ACHOQV_003642"
     CDS             complement(11557..13164)
                     /gene="dppA"
                     /locus_tag="ACHOQV_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFP9631942.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(13909..14042)
                     /locus_tag="ACHOQV_003643"
     ncRNA           complement(13909..14042)
                     /ncRNA_class="other"
                     /locus_tag="ACHOQV_003643"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         3321 a         3827 c         3510 g         3557 t
ORIGIN      
        1 aatcccctac ttttgttgcg caaactctgc cagcaacggc cagcgtttta atgccgcggc
       61 caactgttcg cgttgggaaa tatcggcata atcaatatca ggcagtgaaa atgcctgctt
      121 taacgccaaa atatcattct ggaagatata gcctatcgcg ggatcaggca gagtatctgg
      181 ttcattgtta ttcatttttc ttgatcccta tgaaataacc gtaccgttca tgagacaatt
      241 ctaactgtga gcatttcgtc ggtttattgt ttacggcgag acgggaatgt gctcactttt
      301 tgactttccg ttacgcaaca gaaaaagtta aaaacgaaaa gactaaaatc acatgtcttg
      361 tgacctgcta aattgattca catgctaaat ctgataagtt ttaatttcaa tggtaggttt
      421 atttcttagc tttcgctagt aaactgataa tcagacaaaa tgttgacatg agggacattg
      481 tggaccctgt attctctatc ggtatctcat cattatggga tgagctgcga catatgccag
      541 caggcggcgt ctggtggttt aacgtcgatc gccatgaaga tgctatcagt ctggcgaatc
      601 aaacaattgc atcccaggct gaaaccgcac acgtcgcggt cattagcatg gacagcgatc
      661 cagcgaaaat ctttcaatta gatgattctc aagggccggg aaaaataaca ttattttcaa
      721 tgctaaatca tgaaaaaggt ctatactatt tggcccgtga tttgcagtgt tctattgatc
      781 cccataatta cctttttatt cttgtttgcg caaataacgc atggcaaaac attcctgccg
      841 agcggcttcg ctcatggttg gataaaatga ataaatggag ccggctaaac cattgttcgc
      901 ttttggtaat taattccgga aataataacg ataaacaatt ttcattgtta cttgaggaat
      961 accgttcact ttttggtctt gccagtttgc gttttcaggg cgaccaacat ttgctggata
     1021 ttgctttctg gtgcaacgaa aaaggggtca gcgcccgtca gcagcttagc gttcagcaac
     1081 aaaatggttg ctggacatta gttcaacacc aggaggcgga aatccaacca cgcagcgacg
     1141 aaaaacgcat tctgagtaat gttgctgtac tggaaggtgc gccgccgcta tcggaacact
     1201 ggcaactgtt caacaataac gaagtcctgt tcaatgaagc ccgtaccgct caggcggcga
     1261 cggtggtctt ttctttacag caaaatgcgc aaatcgagcc actggcccgc agcattcata
     1321 ctctgcgtcg ccagcgcggt agtgcgatga aaatcctcgt acgggaaaat accgctagcc
     1381 tgcgcgccac cgatgaacgt ttattattgg cctgcggtgc aaatatggtt atcccgtgga
     1441 atgcgccact ctcccgctgt ctgacgatga tcgaaagcgt gcaagggcag aagtttagtc
     1501 gctatgtgcc ggaagatatc actaccttgc tgtcaatgac ccagccgctc aaactgcgtg
     1561 gtttccagaa gtgggatgtg ttctgtaatg ccgtcaacaa catgatgaat aaccctctat
     1621 tacctgccca cggtaaaggc gttctggttg ccctacgtcc ggtaccgggt atccgcgttg
     1681 agcaagccct gacgctatgt cgccctaatc gcaccggcga tatcatgacc attggcggta
     1741 atcggctggt gctgtttctc tcattctgtc ggattaacga tctggatacc gcgttgaatc
     1801 atattttccc attgcctact ggcgacattt tctcaaaccg tatggtctgg tttgaagatg
     1861 atcaaatcag tgccgagctg gtgcagatgc gcctgcttgc cccagaacaa tggggcatgc
     1921 cgctgccttt aacgcaaagt tctaaaccgg tcatcaatgc cgagcacgat ggtcgccact
     1981 ggcgacgaat accagaacca atgcgactgt tagatgatgc tgtggagcgc tcatcatgat
     2041 gaccatcagc gatatcattg aaattattgt cgtttgcgca ctgatatttt tcccgctggg
     2101 ctatctggcg cggcactctt tgcgacgcat tcgcgacacc ttacgtttgt tctttgctaa
     2161 acctcgttat gttaaaccgg ccgggacgtt acgccgcacg gaaaaagcca gggcaaccaa
     2221 aaaatgactc aatttacgca aaataccgcc atgccttctt ccctctggca atactggcgc
     2281 ggcctttccg gctggaactt ctattttctg gttaagttcg gcctgttgtg ggcgggatat
     2341 cttaacttcc atccgctcct caatttggtc tttgccgcgt ttctgctgat gcccattccg
     2401 cgctacagcc tgcatcgctt gcgccactgg attgccctgc cgatcggctt tgctttgttc
     2461 tggcatgaca cctggttgcc tggcccggaa agcataatga gccagggttc gcaggtggcg
     2521 gggttcagta ccgattattt aatcgacctt gtcacacgct ttattaactg gcagatgatt
     2581 ggggccattt ttgttttatt cgtggcctgg ttattcctgt cacaatggat tcgcattacc
     2641 gtttttgtgg ttgccattct gctatggctg aacgtactta ccctggcggg accaagtttc
     2701 tccttgtggc cagccggtca accgacgacc actgtaacaa cgacgggtgg taacgcagcg
     2761 gcaaccgttg cggcgacggg tggcgcaccg gtagtgggtg atatgcccgc acaaactgca
     2821 ccgccaacaa cggcgaacct taacgcctgg ctaaataatt tctataacgc ggaggcgaaa
     2881 cgtaaatcga ccttcccgtc ttcgctgccc gctgatgctc agccatttga actactggtg
     2941 attaatatct gttcgctttc ctggtcggat attgaagccg ccgggttgat gtcgcatccg
     3001 ctgtggtcgc atttcgatat tgagttcaag aactttaact ccgccacctc ctacagtggc
     3061 ccggcggcga tccgtttact gcgcgccagc tgcgggcaga cttcgcacac taatctgtat
     3121 caaccggcaa ataacgactg ctatctgttt gataacctgt cgaaactggg ctttacccag
     3181 cacctgatga tgggacataa cggccagttc ggcggttttt tgaaagaagt tcgcgaaaat
     3241 ggcggcatgc agaccgaatt gatggatcaa acaaatctgc cggttatttt gctgggcttt
     3301 gatggttcgc cggtttatga cgataccgcc gtgcttaacc gctggctgga cattaccgaa
     3361 aaagataaaa acagccgtag tgccacgttc tacaacacgc ttccactgca tgacggcaac
     3421 cattatccgg gcgtcagcaa aacagcggat tacaaagcgc gggcgcagaa attctttgat
     3481 gaactggacg ccttctttac tgaactggag aaatcgggtc gtaaagtgat ggtggtcgtg
     3541 gtgccggaac acggcggcgc gctgaagggc gacagaatgc aggtatctgg cctacgtgat
     3601 atccctagcc cgtctatcac agacgtcccc gttggggtga aattcttcgg catgaaggca
     3661 ccacatcagg gggcaccgat tgtcatcgac caaccgagca gcttcctggc tatctccgat
     3721 ctggtggttc gcgttcttga tggcaagatt ttctccgaag acaatgttga ctggaaaaaa
     3781 ctcaccagtg ggttgccaca aacagcaccg gtctccgaga actcaaatgc agtagttatt
     3841 caataccagg ataaaccgta cgttcgcctg aacggcggcg actgggtgcc ttacccgcaa
     3901 taagaacgaa aaaggccgca gagtttcacc cctgcggcct gatccgggcg caaattgcca
     3961 ttacggcagc ctgacgcccg catgacacgt tacttccgct tgttcagcca gttcacgatc
     4021 atgctggcaa gaatgccagc aatgaccgga gccgctaaat catgccagaa ggccatgccc
     4081 agctctgcga gcgtcatata gccgcctgag ttgtaatgac aacgtttcgc ggctattctt
     4141 gagttgtttg ggttcaagaa tggcccccgt ggtgttgtca ggtgcatacc tgcaacgtgc
     4201 gggggttttc tctctccagc aaacaatgcc accagggatt aagcccccgc aacattgcgc
     4261 ctcaccggaa cctttccggc ttgccgctga ttctacgact gtttttctgc tccggaatcc
     4321 gtcacgtctt cactctgtgc tgcgcattcc gcagaatatt atccgcttgc aaccgttgca
     4381 tttttcttac gaaaaaggcc gcagagtttt atccctgcgg cctgatccgg gcgcaacttg
     4441 ccattacggc agcctgacgc ccgtctgaca cgttacttcc gcttattcag ccagttcacg
     4501 atcatgctgg caagaatgcc agcaatgacc ggagccgcta aatcatgcca gaaggccatg
     4561 cccagctctg cgagcgtcat atagccgcct gagttgtaat gacaacgttt cgcggctatt
     4621 cttgaatggt ctagatgcaa gaatggcccc cgtggtgttg tcaggtgcat acctgcaacg
     4681 tgcgggggtt ttctctctcc agcaaccaat gccaccaggg attaagcccc cgcaacattg
     4741 cgcctcaccg gaacctttcc ggcttgccgc tgattctacg actgtttttc tgttccggaa
     4801 tccatcactt cttcactctg tgctgcgcat tccgcagaat attatccgct tgcaaccgtt
     4861 gcatttttct tacgaaaaag gccgcagagt tttatccctg cggcctggtc cgggcgcaaa
     4921 ttgccattac ggcagcctga cgcccgtctg acacgttact tccgcttatt cagccagttc
     4981 acgatcatgc tggcaagaat gccagcaatg accggagccg ctaaatcatg ccagaaggcc
     5041 atgcccagct ctgcgagcgt catatagccg cctgagttgt aatgacaacg tttcgcggct
     5101 attcttgagt tgtgtaggtt caagaatggc ccccgtgatg ttgtcaggtg catacctgca
     5161 atatgcgggg gttttctctc tccagcaacc aatgccacca gggattaagc ccccgcaaca
     5221 ttgcgcctca ccggaacctt tccggcttgc cgctgattct acgactgttt ttcagctccg
     5281 aaatccgtca cttcttcact ctgtgctgtg cattccgcag aatattatcc gcttgcaacc
     5341 gttgcatttt tcttacgaaa aaggccgcag agttttatcc ctgcggcctg atccgggcgc
     5401 aaattgccat tacggcagcc tgacgcccgt ctgacacgtt acttccgctt gttcagccag
     5461 ttcacgatca gactggcaag aatgccagct atgaccggag ccgctaaatc atgccagaag
     5521 gccatgccca gttctgcgag cgtcatatag ccgcctgtgt tgtaatgaca acgtttcgcg
     5581 gctattcttg agtggtctag gttcaagatt agcccccgtg atgttgtcag gtgcatacct
     5641 gcaacgtgcg ggggttttct ctctccagca accaatgcca ccagggatta agcccccgca
     5701 acattgcgcc tcaccggaac ctttccggct tgccgctgat tttacgattg ctttttaccg
     5761 tcagcacccg gcaatcttcc tcattgcgtg atgccttcca gcttaatttc tttatttgca
     5821 ttagttgcag ttcatatccg ggctgactcc agactggtag tgaaatgcgc aattttgtag
     5881 aattgcgcct cctcgcgacc tccccagtga atctgttgcc tcatttttta catactgcgt
     5941 attcgacttc tccacctgtt gcgcaagaga aactgggttt attcattttt gcgaggccga
     6001 cttctttctg gacaggactt tatgcagcac aacacactac cgaaacacga tcagaaaatg
     6061 ccgtttacac gctacgactt cggctgggtt ttattatgca taggcatggc gattggtgcc
     6121 ggaaccgtgc tgatgccagt acaaattggc ttgaagggaa tttgggtatt tattaccgca
     6181 gcgatcattg cttatcctgc cacctgggta gtgcaggaca tttatttaaa aaccctttct
     6241 gaaagcgatt cctgtaatga ctacaccgat attatcagtc attacctggg gaagaactgg
     6301 ggaattttcc tcggggttat ctactttttg atgattatcc acgggatttt tatctactct
     6361 ctctccgtgg ttttcgacag cgcctcgtac ctgaaaacct tcggtttaac cgatgccgat
     6421 ctttcacaat ctctacttta taaagtcgcc attttcgccg tactggtggc aattgcgtct
     6481 ggtggtgaac gattactgtt taagatttcc gggccaatgg tggtggtcaa agtagggatt
     6541 attgtcgtct tcggttttgc gatgatcccg cactggaatt tcgccaatat aaccgccttc
     6601 ccgcaagcct ccgtcttctt ccgcgacgtc ctgcttacta ttcctttttg cttcttttct
     6661 gcggtattta ttcaggtgct aaacccgatg aatattgcct accgcaaacg ggaagcggat
     6721 aaagtactgg caacccggct cgcgctgcgt acccaccgaa ttagttatgt cacgctcatc
     6781 gcggtgatcc tattttttgc cttttcgttt accttctcaa ttagccacga agaagccgtt
     6841 tctgcctttg aacaaaatat ctcggcactg gcactggccg cgcaggtgat ccctgggcat
     6901 atcattcata tcacctcgac ggtgctgaat atctttgccg tactgaccgc attctttggc
     6961 atttatctcg gtttccacga ggccattaaa ggcattattc tcaatctgtt aagccgaatt
     7021 attgatacca agaaaattaa ctcacgcatg ctgactctgg cgatctgcgc ttttatcgtc
     7081 attacgttga cgatttgggt ctcgtttcgt gtatcggtgc tggtgttctt tcagttggga
     7141 agcccgttat atggcattgt gtcgtgcctc attccatttt tcctgatcta taaagtcgca
     7201 caactggaaa aacttcgcgg gtttaaagcc tggctgattc tgctttacgg cattttgcta
     7261 tgcttgtcgc cactgttgaa gctgattgag taaaccggag cgcacggccc cggttttgtg
     7321 agttaacgct gcggattttc atcctgatca acagcaaaac aagctaccag ttgaccgccg
     7381 tagtctttta gctgcggctg caactgggtg caggggccga agcgccgacg acagcgggcg
     7441 ttaaacgcgc agcccggcgg tggattcagt gggcttggca gttcaccggt gagcttgatg
     7501 cgctcgcggc gatcgtccgg gttcaggcgc ggcgtcgcgg agagtagcgc ctgagtgtac
     7561 ggatgacgcg ggttattgaa gatttggtcc ttcgttccct tctccacgca gcggcccagg
     7621 tacatcacca tcacttcatc agcaatgtgc tctaccaccg acaggtcgtg cgagataaag
     7681 acataagaca gccccaactc ctgctgcaaa tccatcatca gattcagcac ctgcgcgcgc
     7741 actgagacgt ccagcgcgga aaccggctca tcggcaatca ccacatccgg gtcgagcatc
     7801 agacctcggg caatggcgat acgctgacgc tgaccgccgg agaacatatg cggatagcgg
     7861 tcataatgtt cggttttcag gccgactttc gccatcatcg acagggcttt ttcccgacgc
     7921 tgttctttgc ttaagctggt attaatcagc agcggctcct caagaatttg cccgactttt
     7981 ttacgtggat tcagcgaacc gtaagggttc tggaagacaa tctggatttt ctgccgacgc
     8041 agcttctgcg cctgcggatc gtgcttgagc agatcctgcc cttggtaata cagttcacca
     8101 ccggtgggcg tttcgatcat cgtcagcaac cgaccgaggg tcgatttacc gcagccagat
     8161 tcccctacta ccgccagcgt tttgccgcgt tcaaggttaa acgaaacgcc gtccagcgct
     8221 ttaaccaaac gttccggcgc gaacatacct ttcttcaccg gataatgttt tttcaggtcg
     8281 atagcctgca acagcggttg ttgcgaggtg gcctcttgcg tactcatagt gtcggcctcc
     8341 cggcatcatc aagtgggtaa tggcatttgg actgacgccc gtcagcaagc atattcagcg
     8401 ccggttcttc agcgcgacat ctgtcagtgg cataggggca gcgcgggtta agcaggcagc
     8461 cgttcgggcg gtcatacttg ccgggaacaa cacctggcaa cgacgccaga cgttctttat
     8521 cctgagcaaa ttccggcagc gcacgcagca atgcctgagt atacgggtga cgcggcgcat
     8581 ggaagatggc gtgcgcatcg cccgtttcca ccacctgacc ggcatacatc acgatgattt
     8641 tatgtgccgc ttccgccacc agcgccaggt catgggtaat taacaccagc gccatgttct
     8701 ctttctgctg tagctccagc agtagttcaa tgatttgcgc ctgaatggtc acgtccagcg
     8761 cagtggttgg ttcatcggca atcagcagtt ttggccgaca ggcaatcgcc atggcaatca
     8821 tcacgcgctg gctcatgccg ccggaaagct gatgcgggta aacatccaga cgcgaagccg
     8881 gatcgggaat accgacctga ttcaacaagt caatcgcccg ctgacgacga gtgctcttat
     8941 taccgccctg atgcacctta atcgcttcca taatctggaa acccacggtg tagcacgggt
     9001 taaggctggt catcgggtcc tggaagatca tcgccacttc ggcacccaca aggttgcggc
     9061 gctctttttc cgagatacgc tgcaaatcct gaccgttaaa ctccagcttt tccgccatta
     9121 cgcggcccgg ataatcaatc agccccataa tcgccagcga actgaccgat ttaccagagc
     9181 cggactcacc cacaatcccg accacttcgc cctgttttac gctgtagctg atgcggtcta
     9241 cggcgcggaa cggtgcgctt tcgtcgccga aatgcaccga taatttatct acatttaata
     9301 acgccatctc gaacctctta ctgcttcagt ttgggatcga gcgcgtcacg cagaccgtca
     9361 cccatcaggt taaatgccag caccgtcagc aggatcgcca gacccgggaa ggtcacgacc
     9421 caccaggcgc tttgcgcgaa ctgcaacacg tcggagagca tggtgcccca ctccggcgtt
     9481 ggcggctgcg cccccatgcc gaggaaacca agagcagcca tatcgagaat ggcgttagag
     9541 aaaccgagcg acgcctgaac aatcagaggc gcaaggcagt tcgggaagat gttaataaac
     9601 atctgacgca tcgccccggc acctgccacg cgagacgcgg taacgtaatc gcggttaact
     9661 tccaccagca cggcggcgcg ggttaagcgc acatagtgcg gcagggcaac aaaggtcagc
     9721 gccagcgcgg cgttaccaat cgacgggccg aaaatcgcca ccagcaccag tgccaacagc
     9781 agacttggca gcgccagcat gatatcgacc acgcgcatga tgatgttatc gaccaggccg
     9841 ccgaagtaac cggcgatcag gccgagaata acgcccataa tcagcgataa cacaactacc
     9901 agacagccaa ccagcagtga caatcgcgca ccgtacatca gacgcgacag cacatcacgg
     9961 cctacgtcat cggtgcccag caagtgcgcc atgctgccgc cttcctgcca ggctggcggg
    10021 gcgagcagtg catcgcggaa ctgttccgcc gggttatagg gtgcaatcca gttggcaaag
    10081 atcgcgataa acagcacgat gacgacgtaa accagcccga cgaccgcgcc tttgttgcgt
    10141 ttaaaatagt gccagaactc ctgtaacggg gtcatcggca ccggtgcgct aatcacttta
    10201 ttttcagtaa cctgtgacat gatgtcccct tacttcttat gacgaatacg cgggttcacc
    10261 acgccgtaca gcagatcgac cagcaagttg acgaggataa tcatcgtcgc caccagcaat
    10321 acgccgccct gcactaccgg atagtcgcgg cgttgcagtg cgtcaatcaa ccagcgcccc
    10381 agaccgggcc acgagaagat ggtttcggtc agaatcgccc ccgccagcaa cgttcccacc
    10441 tgcaggccga taacggtcac caccggcagc atcgcgttac gcagcgcatg gacgataatc
    10501 accctaaggc gggtcaaccc tttggcgcgc gcggtgcgga tgtaatcttc acccagcact
    10561 tccagcatcg aggagcgtgt catacgcaca atgaccgcca gcgggatagt acccagcaca
    10621 atggcgggca agatcatatg ggcgacggca tcgataaagt taccgtcttc accccagatg
    10681 gcggtgtcga tcagcataaa accggttaac ggattggaat catcgaggaa caccatgtcg
    10741 ctcacgcgac cggagacggg cgtcaggttc cagtgcaccg aaaccagcat gatcagcatc
    10801 atgccccacc agaagatagg cattgaataa cctgtcagcg ccaggccaac cgccgtgtga
    10861 tcgaaaatgg aaccgcgttt aaccgcagcc agcacaccaa ccggaatacc gactgccgta
    10921 gcaaaaatca tcgcgcagac gccaagttcc agcgtggcct ggaagcgcgg cacgaactct
    10981 tcccataccg ggatgcggct tttcatcgaa atgcctaaat cgccgtgcat tacgccccaa
    11041 atgtaatgga gatactgctg ccacatcggt ttatctaagc cgagttcagc cagcagctgc
    11101 gcgtgacgct ctggggagat cccacgctcg cccgccatga tcatcaccgg atcgcccggg
    11161 atcatgtgga caaaggcaaa tgtgagaagg gtaataccga taaacgtggg gatgacgagt
    11221 cccaaacgtc ggagaataaa ctgcaacata acccggattc tctataatga cccgcagcct
    11281 ggaactgcgt gtctgtattg ctcacaaatc aattcccgcc cttgtggaac agggaatgaa
    11341 gtattgctgc cggatggcgc tgcacgcaga ggcacagcgc caccgggtat gcagattgtt
    11401 gacaaagagc gctttgttta tgccggatgc ggcgcaaacg ccttatccga cctacaaaat
    11461 cgtgcaaact caatacattg cagagttttc gtaggcctga taagcggagc gcatcaggca
    11521 gttttgcctt tgccatcagt cttgtatggc ttttaattat tcgatagaga cgttttcgaa
    11581 gtgatgtttg cctaatggat caaccacata gcctttaact tctttgcgta ccggttcaaa
    11641 cacggtggag tgagcgatga tcagtgccgg agcctgatcg tgcatcacca cctgcgcttg
    11701 cttgtacagt tcaacgcgtt tgttatggtc gtcggtagca cgcgccggtt gaatcagatc
    11761 ttcaaacggt ttgtagcacc attttgagta gttggagcct tgttcagagg cggcgcagct
    11821 gaacagggtg gcgaagaagt tatccggatc cccattatcg ccagtccagc ccatcattac
    11881 cgtctggtgc tcgccatctt tcgcacgctt gaggtactca ccccattcgt aggtgacaat
    11941 tttggcctgc acgccgactt tcgcccagtc tgcctgaatc atctccgcca tgcggcgagc
    12001 gttcgggtta tacggacgtt gtaccggcat cgcccacagg tcgatggaga aacctttttc
    12061 cagacccgct tctttcagca aggctttcgc tttttcagga tcgtaggtgt agtcctgaac
    12121 gtcgtcgtta tagccccaca tggttggcgg gatcaggttt ttcgctgata cgcccgcgcc
    12181 ctgataaacc gctttgatga tcgcgtcttt gttcaccgcg taggtcagag cctggcgaac
    12241 tttcacgtca tcgagtggtt ttttctgcac gttatacgag agataaccga cgttcagccc
    12301 cggcatttcc atcaggttga tggatttatc ctgcttcatg cgggcgatat ctgccgggtt
    12361 cgggtacggc atcacctggc attcgttctt ctgcaatttc gcataacgca cggaagcgtc
    12421 aggagtaata gagaagacca gcgtatcgat cttcggtttg gtgccccagt agccatcaaa
    12481 cgctttatag cgaatacggg aatctttctg gtactgctgt aactggaacg ggccggtacc
    12541 gattgggttg aggtccagtt tttctggtgt accggctttc atcatcgcgt cagcatattc
    12601 ttttgacaga atagaggcga agtccattgc caggtcagcg aggaacggcg cttccgggcg
    12661 agtcagcaca aactgaacgg tgttatcgtc cacctttttc acttcgctga tcagctctgg
    12721 taagcccatg ccttcgaagt attcgtagct gccgccagag actttatggt acgggttttg
    12781 cgcgtttttc tgacgatcga acgagaacac tacgtcatcg gcattcagtt cgcgcgtcgg
    12841 tttgaattct ttattgtcgt gccacttcac acctttacgc agatggaagg tataggtttt
    12901 accgtcttcg ctgacttccc acttttcagc gaggcccggg atcacttcag tggtgccgat
    12961 tttaaattca accagacggt tataaagcgg tacggaagag gcgtcatagg tggtgccgga
    13021 ggtaaacagc tgcgggttaa acccttccgg agatccttct gagcaataaa ccagagtttt
    13081 agcctgaaca cttgctgcga cggtcatagc caccaggctg agaccaagct tcagcatccc
    13141 tgactttttc aaggaaatac gcattattct gctccaattg tgatgtttgt tgttttaacc
    13201 ctttgcagtg ggttgtcgct gcctgacctt ttttgttttt tgcccggtcg ggtcaacgtt
    13261 atgaggtggg gatgccgtac taattaacat cagtgtaaca gtgcggatcc tccataaaat
    13321 gcccctcgtg acctacaatc tgtcaacaga atgtgaaaac gtcaatacag ccaaccggga
    13381 tttacaccaa cggtgagaat ccacaaacaa agattaaaaa aacttcaaac agctatttgc
    13441 agcagagaaa tttgtgctac gccaacacaa ccagaacaat cagcttaata tttagcaaca
    13501 tttggtgatt aagttttatg catttaatga aaaaaatctg aggaaaaggt ggatatctga
    13561 gcaattaata ccatgaacgc taaaacgcac agcggaaaat gccagcgcca gccataagta
    13621 acgcaaaaaa agatttaagc aaatataaaa aaagacaatg gtttctgtca caaatccgtt
    13681 aatggatagt gagatatggg gcgcaaattt gacagggaaa tgtgtggtgt gtagatcgaa
    13741 taaggcgttc acgccgcatc cgacaataag tgcggtgccg ttgcctgatg cgacgctggc
    13801 gcgtcttatc agacctacgt cgagtcgaac ctccccgccc ccgggggttc tcttcctcat
    13861 gagctacaga tgcaaaaaag cctgctcgtt gagcaggctt ttcgaatttg gtcggtgata
    13921 gaggattact cgccacttcg tggctcgccc tgcgggccgt tgctgacgca acgttctctc
    13981 gcttcgctcg agtcgaacct ccctgccccc ggaggttctc atcctcatag gctacaaatg
    14041 caaaaaagcc tgctcgttga gcaggctttt gatagagtat tcaaacgtag cttgtaacca
    14101 aatgaaaaat aataatattg ataaccacaa tccaaaatcg tatatccaaa cgtatagcta
    14161 aaatgatttg cttaaaaaat gtacaacaat gaaccggcat tttcctggtt atccg
//
LOCUS       DAZKGF010000128        14049 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZKGF010000128 DAZKGF010000000
VERSION     DAZKGF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.128"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..604
                     /locus_tag="ACHOQV_003644"
     CDS             38..604
                     /locus_tag="ACHOQV_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9631943.1"
                     /translation="MFKGQKTLAALAISLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSNNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            655..3114
                     /gene="ybgQ"
                     /locus_tag="ACHOQV_003645"
     CDS             655..3114
                     /gene="ybgQ"
                     /locus_tag="ACHOQV_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9631944.1"
                     /translation="MDTVNIYRLSFISCLVMAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKGKGVIVPGEYFVSVAVNNNQISNGQKIDWKKNGDQTIPCINDLLVDKFGLKPEVRQ
                     SLPRLNQCVDFSSRPEILFIFDQASQQLNITIPQAWLAWHSDNWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSNSTNLNAYGTAGINAGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTVSHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRANNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRTSMSHQTENETFFSNEVSWGMLSNTSLYGGLLISGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSQFDTQQDERGLSYRFNYSKQVDATDSTISLA
                     AYRFSDRHFHSYANYLDHKYNNSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGNWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSTGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDNSGISVGMVGEEGHAWLSGVAENQQFTVIWGDSQR
                     CSLHLPEHMEDTANRLILPCH"
     gene            3129..3860
                     /gene="ybgP"
                     /locus_tag="ACHOQV_003646"
     CDS             3129..3860
                     /gene="ybgP"
                     /locus_tag="ACHOQV_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9631945.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPSPEKNSGHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVITNTGSYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCRLQAVEAKK"
     gene            3857..4918
                     /gene="ybgO"
                     /locus_tag="ACHOQV_003647"
     CDS             3857..4918
                     /gene="ybgO"
                     /locus_tag="ACHOQV_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9631946.1"
                     /translation="MSAGKGVLLVICLVFLPLKSALALNCYFGTSGGTVEKSEAIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDASLGKSRIDTNQCIDSKNINIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREIPPHDYQSSLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLIEEDKALLIGNGLKLRLLDENSSPYTFNKYSEYADFTSDMLIYEKTYTAELSSIPG
                     TPIEAGPFDTVVLFKINYN"
     gene            5071..6117
                     /gene="abrB"
                     /locus_tag="ACHOQV_003648"
     CDS             5071..6117
                     /gene="abrB"
                     /locus_tag="ACHOQV_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9631947.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATNPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6114..6905)
                     /gene="nei"
                     /locus_tag="ACHOQV_003649"
     CDS             complement(6114..6905)
                     /gene="nei"
                     /locus_tag="ACHOQV_003649"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFP9631948.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKPYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTMRVLRVKLQTVDKTIL
                     LYSASDIEMLTPEQLITHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6941..7675)
                     /gene="pxpA"
                     /locus_tag="ACHOQV_003650"
     CDS             complement(6941..7675)
                     /gene="pxpA"
                     /locus_tag="ACHOQV_003650"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFP9631949.1"
                     /translation="MKIDLNADLGEGYASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSVMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            complement(7665..8597)
                     /gene="pxpC"
                     /locus_tag="ACHOQV_003651"
     CDS             complement(7665..8597)
                     /gene="pxpC"
                     /locus_tag="ACHOQV_003651"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFP9631950.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8591..9247)
                     /gene="pxpB"
                     /locus_tag="ACHOQV_003652"
     CDS             complement(8591..9247)
                     /gene="pxpB"
                     /locus_tag="ACHOQV_003652"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFP9631951.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGIYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9270..10013)
                     /gene="ybgI"
                     /locus_tag="ACHOQV_003653"
     CDS             complement(9270..10013)
                     /gene="ybgI"
                     /locus_tag="ACHOQV_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFP9631952.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10284..11765
                     /gene="dtpD"
                     /locus_tag="ACHOQV_003654"
     CDS             10284..11765
                     /gene="dtpD"
                     /locus_tag="ACHOQV_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFP9631953.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFSHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDIFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11808..13226)
                     /gene="phrB"
                     /locus_tag="ACHOQV_003655"
     CDS             complement(11808..13226)
                     /gene="phrB"
                     /locus_tag="ACHOQV_003655"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFP9631954.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSASVLALYIATPRQWAA
                     HNMSPRQAEFINAQLNGLQIALAEKGIPLLFHEVDDFAASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVQAERALRNVVCEGFDDGVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIDPAPSITLNYPRQPFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSIWLNEL
                     IWREFYRHLITYYPSLCKHRPFIVWTDRVQWQSNSAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13223..13732)
                     /gene="ybgA"
                     /locus_tag="ACHOQV_003656"
     CDS             complement(13223..13732)
                     /gene="ybgA"
                     /locus_tag="ACHOQV_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFP9631955.1"
                     /translation="MNQQRFDESTLIRIFALYELHRLKEHGLTRGALNDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRGGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13874..14049)
                     /locus_tag="ACHOQV_003657"
                     /pseudo
     CDS             complement(13874..14049)
                     /locus_tag="ACHOQV_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3377 a         3763 c         3438 g         3471 t
ORIGIN      
        1 tcgcaacact ctatctcatt atttaaagga ctcagctatg tttaaaggac aaaaaacatt
       61 ggccgcactg gccatatctc tgctattcac agcacctgtt tatgcggctg atgaaggatc
      121 cggtgaaatt cactttaaag gtgaagttat tgaagcaccg tgtgaaatac atcaggatga
      181 tattgataaa gaggttgaac tcggtcaggt gaccaccagc cacattaatc agtcgcatca
      241 cagcgatgcc gttgctgtcg acctgcgctt agtcaactgc gatctggaaa actccaacaa
      301 cggttccggc ggcaagattt ctaaagttgc agtcaccttt gacagctcag cgaaaaccac
      361 cggcgcagat ccgattctca acaacaccag cacgggtgaa gcgaccggtg tcggcgtgcg
      421 tttaatgaat aaagatcaaa gcaatatcgt tttaggcacc gctactccag atatcgacct
      481 ggcaccaacc tccagcgaac aaacgttaaa tttctttgcg tggatggaac aaattgatca
      541 ggctacgccc gtaacaccag gcgcagttac ggcgaatgcg acctacgtac tggattataa
      601 ataagtatca ctttggcggc gtcatcgtcg cctttcttca aatatttatc aggtatggac
      661 accgtgaata tttatcgact ctcttttata tcctgtctgg tcatggcgat gccttgcgca
      721 ttggcggtcg aattcaacct gaatgtcctc gataaatcga tgcgagatcg cattgatatt
      781 tcattattaa agggaaaagg ggtcattgtt cccggtgaat attttgttag cgttgcagta
      841 aataacaacc aaatcagtaa cggacaaaaa attgactgga aaaagaacgg tgaccaaacc
      901 attccgtgta ttaatgattt actggtagat aaatttggtt taaaacctga agttcgtcag
      961 tcgttaccac ggttaaacca gtgcgtcgat tttagctccc ggcctgaaat tctcttcatt
     1021 tttgatcagg ctagccagca actgaatatc actattccac aagcctggct ggcgtggcac
     1081 tcagataact ggactccccc ctcaacatgg aaagaaggtg tcgccggcgt cctgatggac
     1141 tacaacctgt ttgcaagcag ctaccgccca caggatggta gcaacagcac taacctgaac
     1201 gcctatggaa cagccggaat taacgcggga gcatggcgtt tacgcagtga ttaccaattg
     1261 aatcagaccg atagcgatga taaccatgaa cagtcaggcg aaatatcacg cacctatctt
     1321 tttcgtccat taccgcaatt aggttcaaaa ttaacccttg gtgaaacgga tttcagttcc
     1381 aatattttcg acggcttttc ttataccggc gcggcactgg caagtgacga tcggatgtta
     1441 ccgtgggagc tacgcggcta cgccccacaa atcagcggca ttgcacaaac caacgccacc
     1501 gtcaccgtga gtcactcagg gcgagttatt taccagaaga aagtcccacc aggtccgttt
     1561 atcattgatg accttaatca gtccgtccag ggtacgttag atgtcaaagt gacggaagaa
     1621 gatggtcggg cgaacaattt ccaggtttcg gcagcatcga cgcctttcct tacgcgccag
     1681 gggcaggttc gctataaact tgctgccggt cagccgcgaa catctatgtc tcatcaaact
     1741 gaaaatgaaa ccttttttag caatgaagtt tcctggggga tgctgtcaaa tacctcactg
     1801 tacggcggcc tgctgatttc gggtgacgac taccattctg ccgcaatggg tatcgggcaa
     1861 aatatgctgt ggcttggtgc attgtcgttt gatgtcacgt gggccagtag tcagtttgat
     1921 actcagcagg acgagcgggg cttaagttac cgctttaatt acagcaaaca agtggatgcc
     1981 accgacagca cgatttcgct cgccgcttat cgtttctccg atcgtcattt tcacagctac
     2041 gccaactatc tggatcacaa atacaacaac agcgatgcgc aggacgaaaa acagacgatc
     2101 agcttatccg tgggccaacc gattacccca ctgaacctca atctgtacgc caacctgctg
     2161 catcaaacct ggtggaatgc agacgcctcc acgaccgcca acatcacggc gggttttaat
     2221 gttgatattg gtaactggag agatatctcg atctcgacgt cattcaatac aacccattac
     2281 gaagataaag atcgcgacaa tcagatttac ctgtcgattt cgctcccctt cggtaacggg
     2341 gggcgggtcg gttatgacat gcaaaacagt agtcacagca ccacgcaccg catgtcgtgg
     2401 aatgatacgt tagatgaacg taatagctgg ggcatgtcta ccggactgca atccgaccgt
     2461 ccggataatg gagcccaggt gagcggtaac tatcagcacc tgagttcagc gggtgagtgg
     2521 gatatttctg gtacctatgc cgccaatgat tacagttccg tcagcagcag ctggagcggt
     2581 tctttcacgg caactcaata tggtgcggcg ttccatcgtc gcagctccac caatgaacca
     2641 cgtctgatgg tcagtaccga tggcgtggca gatattccgg ttcagggcaa tctcgactac
     2701 accaaccatt ttggcattgc ggtggtgccg ttgatttcca gttatcagcc ttccaccgtg
     2761 gcggtgaaca tgaatgactt acccgacggc gtaacagtgg cagaaaacgt tatcaaagaa
     2821 acgtggattg aaggcgcgat aggttacaaa tcgctggctt cccgttccgg taaagacgtt
     2881 aacgtcatca ttcgcaacgc cagcggtcag ttccctcccc tcggtgcgga tatccgccag
     2941 gataacagcg gcattagcgt ggggatggtt ggcgaggaag gacatgcctg gttaagcgga
     3001 gtcgctgaaa atcaacaatt taccgtgatc tggggtgata gccagcgttg ctcgctccat
     3061 cttcctgaac atatggaaga caccgcaaat cgcctgattt taccttgtca ttaattaagg
     3121 aaatgaaaat gacatttatg aaaggactac cccttttgtt gttggttgcc agtctgtgca
     3181 gccacgctgc actacaaccc gatcgcactc gtattgtgtt taacgctaat gataaagcta
     3241 ccagtctgcg cgtggataat cgtagcgata aactccccta cctcgcttat tcctggctcg
     3301 aaaatgaaaa aggggagaaa agcgatgatc ttctggttgc cctgccaccg atacaacgtc
     3361 tggaaccgaa agcaacaacg caggtacgga tagtaaagca agcctcgacc acaaaattac
     3421 cgggcgatcg cgaaacgcta tttttctaca atatgcgtga aattcctccc tccccagaaa
     3481 aaaacagcgg ccatgccgtt ctgcaagtcg ccattcagag tcgtatcaag gtattctggc
     3541 gaccggctgc attacgcaag aaagcaggag aaaaggtcga actgcagtta caggtcagcc
     3601 agcagggcaa ccaactgaca ctgaaaaatc ctaccgcgta ttatctgacc attgcctatc
     3661 tggggagaaa tgaaaaaggc gttcttcccg gcttcaaaac cgtgatggtt gcgcctttta
     3721 gtacagtaat tacaaacacc ggaagttata gcggcagtca gttttatctc ggttatatgg
     3781 atgattacgg cgcgttacgc atgacgacgc tcaactgcag cggacaatgc cgtttacaag
     3841 cagtggaggc gaagaaatga gtgctggcaa gggagtgttg ctcgtcatct gtctggtatt
     3901 tctgccattg aagtctgcgc tagcgctgaa ctgctatttt ggtacttccg gcggaacggt
     3961 agaaaaatcg gaagcgattc aaccctttgc tgtgccaggt aatgccaaac ccggcgataa
     4021 gatctgggaa tctgacgata ttaaaattcc cgtctattgt gacaacaata ccaacggcaa
     4081 ctttgaaagc gaacatgtct acgcatgggt aaacccctac ccgggggtac aggatcgtta
     4141 ttatcaactc ggcgtgacat acaatggtgt cgattacgat gccagtctgg ggaaaagccg
     4201 catcgacacc aaccagtgta tcgacagtaa gaacattaac atttataccc ctgagcagat
     4261 tatcgcgatg ggctggcaga acaaaatatg ctcgggtgac cccgctaata ttcacatgtc
     4321 gcgaaccttt cttgctcgta tgcggttata cgttaagatt cgcgaaatac cgccgcatga
     4381 ttatcaaagt tcgcttagcg actacatcgt cgtgcaattt gatggtgccg gtagcgttaa
     4441 cgaagaccct accgcccaaa acctgaaata ccatattact ggtctggaaa acattcgtgt
     4501 actggattgc agcgtcaatt tttctatttc accagaaaca caggtgattg attttggtaa
     4561 atttaacctg ctggatattc gccgccacac aatgtcgaaa acattcagta ttaagacgac
     4621 aaaatcacaa aatgaccagt gcaccgacgg atttaaggtc agctcttcgt tttataccga
     4681 agaaacattg attgaagagg ataaagcact gctgattggt aatggactga agctacgttt
     4741 gctggatgaa aattcttcac cctatacttt caacaaatac agcgaatatg ctgatttcac
     4801 cagcgatatg ctgatctacg aaaaaaccta tacggctgaa ctttcgtcca ttcccggcac
     4861 ccccatcgaa gcgggtccct tcgatacagt ggtgcttttt aagattaact ataactaatt
     4921 tatcatgatg ccctttgcag ttagtaaagg gcctctcttt cagaaaattg ctgattttcc
     4981 ccttttcctt gattgctgtt attttttcct ggcccggctt tctttatgct gtatcgatac
     5041 accaaagaaa aaataatgag ggaacgaagg atgccagttt tgcagtgggg aatgttatgt
     5101 gtgctctcac ttctcctttc aattggtttc ctcgcggttc atctcccggc ggcgctatta
     5161 cttgggccga tgatcgccgg gatcatcttt agtatgcgcg gaattactct gcaacttccc
     5221 cgctccgctt ttctcgccgc ccaggccatt cttggctgca tgattgcgca aaacctgacc
     5281 ggttcgatcc tgaccacgct agcggtgaac tggccgattg tgttagctat tttactggtg
     5341 acgttacttt ccagcgccat cgtgggttgg ttattggtgc gctatagctc actgcctgga
     5401 aataccggtg cctggggttc ttctcctggc ggcgcggcgg caatggtcgc tatggcgcaa
     5461 gattacggcg cagatattcg cctggtggcg tttatgcaat atctgcgagt gctgttcgtc
     5521 gcaggggccg ctgttctggt cacccgcatg atgctgggcg ataacgctga agcggttaac
     5581 cagcagatcg tctggtttcc gccagtgagc attaatctcc tgcttacgat tttgctcgcg
     5641 gtcgttgccg gtacagtggg atgcttgcta cgtcttcctt ccggcacgat gctcatcccg
     5701 atgctggcgg gcgcagtgct ccagtctggt cagctcatca ccatcgaact accggaatgg
     5761 ctgcttgcga tggcgtatat ggcaattggt tggcggattg gtcttggttt cgataaacaa
     5821 atcttactgc gggcattgcg cccgctaccg caaatcctgc tgtcgatttt tgctctgctg
     5881 gctatttgtg cgggtatggc gtgggggctg acccggttta tgcatattga ttttatgacc
     5941 gcctacctcg ccaccaaccc cggcgggctt gataccgtgg cggtcatcgc cgcagggagc
     6001 aatgccgata tggcgctcat catggcgatg caaaccctgc gcctgttcag tattttgctg
     6061 acgggacctg ccattgcgcg gtttatttca acctatgcgc cgaagcgttc tgcctaatgc
     6121 tggcagccag ggcaccagta aaacgggcga gatgacagcg tggttttctc aatgatgccg
     6181 ccacagcgtt cgcacagttc gccatcacga tgaaaaacct taaagcgaaa cagcgcccca
     6241 tgatgcttat tctcatccac ctgcccccgc gtagcgtagg aaaatcgagg aatatccagt
     6301 aacgcgtgtg cgagtgcatc cagttgcgcc gcattgagat ctttcgcttt atgatttcca
     6361 gtcaacccaa cctgccagag gatctccacc cgcaaataat tgccaagccc agccagaaac
     6421 gcctgatcga gcagtaatcc agcaaactga cggttacgaa agcgcggcga caataaccgt
     6481 tcctttacca cctccggcgt cagattcgga tccagcacat cggggccgac tcgttgtaaa
     6541 aacggatgcg tgatcagttg ttccggcgtc aacatctcaa tatccgaggc gctataaagc
     6601 agaatagttt tgtcaaccgt ttgcagtttt acccgcaata ctcgcatagt ctgcggctct
     6661 tcgccggtat caaccacgcg ccagacgccg taaagctgat tatggctgta gagcgttaag
     6721 tcgttggaaa aatgagttaa caacgcctta ccacgcgttt ccacatgggt aacgtgttga
     6781 ccgataagtt gtgattgata aggttttaac tgcgggaagg caaaccagac atcagttagt
     6841 ggtttgcctt tgatcgccgc ctccaggtta tccgctgcac ggcggatctc cgggccttca
     6901 ggcatggtga tatccttttt tcattcttta ttaaacgggg ttatgctgcg acaacaatcc
     6961 ccttttcggc aaatgtagag cgtagtcggc gggcgaatgc cagtgcgtgc tcgccgtcgc
     7021 catgcaggca gaccgtttgc gccgtgaccg ttgcccattc gccggtgatg cttttgactc
     7081 tgccgtgttg caccatctcc agcgtttgcg ccagcgcctg ttcttcgttt tcaatcaacg
     7141 cgcctggctg gcttcgcggc accagcgaac cgtctgcctg ataaccgcga tcggcaaaca
     7201 cttcctcgcg cgttgtcagg ccatattgct tgcctgcacg aatcagctcg cttcctgcca
     7261 gcccgacgag aatcaatgcc ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca
     7321 gttgtgcctc tttcgctgcc tggttgtata acatgccgtg aggtttgaca tgacgcataa
     7381 caccgccttg cgcacgggca atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt
     7441 aaacggtttc tggcggcagc tgcatgacgc tgcgcccaaa attttccctg tcgggaaaac
     7501 tcgggtgagc gccaatcgca acaccatttt ttattgcttc acgcacgcaa gcctgcatgg
     7561 tttgcgcatc gcctgcatga aatccacagg caatattggc agaggaaacc agcgttaata
     7621 gctctgcgtc gctggcatag ccttcgccca gatcggcgtt caggtcaatt ttcattgtgc
     7681 agccgccacg ctaattgttc gaaataacgt tgctgatctt gccgtgcttt cagtgcttct
     7741 tccagtgaac actggacaaa atgaatcggc tgaccgagcg gaatttgcgc cagatggtac
     7801 atatcagcct caatgataca ggcaatacgc ggataaccac cggtggtctg tgcgtcgttc
     7861 atcaacacaa tcggctgccc gttatgcggc acctgcacca cgcccggtaa caaaccatga
     7921 gataacagtt cgcgatcggt ggtacgtttt aaaatttgcc cctgtaagcg atagcccatt
     7981 cggttacttt gcgagctaag ctgccagggc gaacgccaga atgcatcctg cgaggcgcga
     8041 tcgaactcat gatattccgg ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt
     8101 ttaacgccct gcgcttccat aaaatcacgc ttcgatttgc caatcgggag tcggtcacca
     8161 tccttcagta aacggccttc cagcccgcca atccccactt tgagatcggt gctgcatgac
     8221 cccattaccg gcggaacatc aataccaccc gcgaccgcca gataactgcg catcccgtgc
     8281 tgcgggcgtt taagcgttaa acgctgacct gctttcatcg gcaagcgcca gccggtccag
     8341 acggcgttat catccagccg cgcttcgcaa ccggcccccg tcagagcaaa ccacccatca
     8401 gtttcgaact caaccgttaa ctgaccgagc gtgatctcca gcgcgggggc attggcgtca
     8461 ttacccacca gtaggttagc aatgcgcagc gcaggcatat ccagtgcgcc gcagtggctg
     8521 atacccgact ggcgaaaacc gtgacgaccg ccatcctgca cagtggtata catacccgca
     8581 cgaataatct tcagcatact ccctccttct gcggtacaaa gcgcacgctg tctcccggac
     8641 gtaataagat gggttcgtca cgcgccggat caaacaggct gagtgaggta tgaccaatca
     8701 actgccagcc acccggcgtt gccaacggat aaataccagt ctgcggcccg ccgatcccga
     8761 cagaacctgc cggaacgagc aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg
     8821 gcaaactccc gagatacggg aagcccggtt gaaaacctaa aaaccagacc acgtattcca
     8881 cggaggagtg caattcaaca acctgttttt cgctcaatcc acaatgcgcc gcgaccaccg
     8941 ccaaatccgg tccggctgcg ccaccgtaaa ccaccggaat ttcaataaag cgagactccg
     9001 gctccagcgc ctcactctcc tcccaccagc gttgcaaacg ctctatggca tccagcgcca
     9061 gcgactcagg attacgcaaa atcactgtga tattgttcat gccaggaatg gcttcaacca
     9121 cattcggcat atccaccaga cgctgtgcca gtcgccagat ccgtttctgg ctagccagcg
     9181 tcaccggcgg ttccagctcc agcactaccg ccgtttcacc tatcagataa caacgcgctc
     9241 gttgcacttt cgatccctct gattattcgt tatgcaggat taggaatatc aataaaggtc
     9301 acatcaagat cggtattttc attcagccac tcgctcaatg cgcgaatacc accgcgttca
     9361 gtggcatggt gacccgcagc ataaaaatgc aatccctgct cgcgggctga atgaatggtc
     9421 tgttctgaaa cttcgccagt aataaaagca tccacgccaa aacgcgcggc gctatcgata
     9481 aaactctgcc cgccgcccgt gcaccaggcg acgcgctgaa ccacctcagg tccggtatcg
     9541 ccacaccata acggcttacg tcccagacgc gcttcaatcc aggaagccag ctccagtccc
     9601 ggcacaggca tggtcagttc gccccacggc accaacggct caatttcgcc catgaccgtg
     9661 atccccagta acgccgccag ttgtgcgtta ttacccagct ccggatgcgc gtccaggggc
     9721 agatgccagc catacaggtt gatatcattc gccagcaacg tttttaaacg gttacgcttc
     9781 attccgcgaa tgaccggaga ttcacctttc cagaagtagc catgatgcac aatgaccgca
     9841 tccgcgccca gacgcaccgc ttcatcgagc aaagcctggc tggcggtgac accggtaaca
     9901 attttttgca ccgtctcttt gccttccacc tgcaaaccat tcggcgcgta atcactaatc
     9961 gccgcgctgt tcagtttttc gttaatcagt tgttccagtt cggtgttttt catcttcgcg
    10021 ccttccgtat tctgatagct acaccgttaa catagcgtta cctcgcctgt tcgtcgatag
    10081 ccaatctttt gacatattaa gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc
    10141 atcacgcttc agctaatgta tatttatgca atcagacagc atattgcata aataatcaga
    10201 tctgtatcaa agttttaact ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg
    10261 ttgcagtcaa ttgtggaatt attatgaata aacacgcatc acaaccgcgc gctatttact
    10321 atgtcgttgc gctgcaaatc tgggaatatt ttagctttta cggcatgcgt gccctgctga
    10381 tcctctatct caccaatcaa ctaaaatata gcgataatca cgcctacgag ttatttagcg
    10441 cctactgttc gctggtatat gtcacgccaa tcctcggtgg ctttttggcg gataaagttc
    10501 tcggcaatcg catggcggtg atgctgggtg cgttgttgat ggcgatcggt catgtggtgc
    10561 tgggtgccag tgagatccat ccgtcattcc tctatctgtc cctggcgatt atcgtttgtg
    10621 gctatggtct gtttaaatcc aacgtcagtt gcctgctcgg cgagctgtat gagccaaccg
    10681 atccgcgtcg tgatggcggt ttctcgctga tgtatgcggc gggtaacgtg gggtctatta
    10741 tcgcacctat cgcctgtggc tacgcccagg aagagtacag ttgggcgatg ggctttggcc
    10801 tggcggcggt tggcatgatc gcgggtctgg tcattttctt atgtggcaat cgtcatttct
    10861 ctcatacccg cggcgttaac aaaaaagtac tgcgtgcgac aaactttctc ctgcccaact
    10921 ggggatggct gctggttctg ctggtggcaa cacctgcgct gatcaccgta ctgttctgga
    10981 aagagtggtc ggtatacgcc ttgattgtcg cgactattat tggcctgggt gtattggcaa
    11041 aaatttatcg caaagcagaa aaccagaaac agcgtaagga gctggggctg attgttaccc
    11101 tcaccttctt cagcatgttg ttctgggcat tcgcacaaca ggggggtagc tcgattagcc
    11161 tttatatcga ccgcttcgtt aaccgcgata tatttggtta taccgttccg actgctatgt
    11221 tccagtcgat taatgccttc gcagttatgc tgtgcggtgt gttcctggcg tgggtggtta
    11281 aagaaagcgt cgcaggtaat cgtaccgtgc gcatctgggg gaaatttgct cttggtctcg
    11341 gcctgatgag cgccggattc tgcattctga ccttaagcgc ccgctggtct gcaatgtatg
    11401 gtcactcttc tctgccactg atggtgttag gcctggcggt gatggggttt gcggaactgt
    11461 ttatcgaccc ggttgctatg tcgcagatta cgcgcattga aatccccggc gtgaccggcg
    11521 tattaaccgg catttatatg ctgctttccg gcgcgattgc gaactatctg gccggcgtga
    11581 ttgccgatca gacatcgcag gcttcgtttg atgcttccgg agcgatcaac tactccatca
    11641 atgcatatat tgaagtattt gatcaaatta cctggggcgc actggcgtgt gtaggactgg
    11701 tgctgatgat ttggctgtat caggcgctta agttcagaaa ccgcgcgctg gcgctggaat
    11761 cttaacaggc cagcaccgat gcctgataag acgctcgctg cgttttatca ggctcctttc
    11821 ctcgcttctt cgtacgctgc cagcgttcgc agccttgcct ctttgtgatc aactatcggt
    11881 tgcggataat ccagcgtcac acctgctttc tgcgcccact tccacggctc atgcaccact
    11941 ttccctggca catcgcgcag ttccggtagc cactggcgga taaactcgcc ctcacggtca
    12001 aatttctcgc cctgggttgt cgggttaaaa atacgaaaat acggcgctgc atcggttccg
    12061 gttgaagcgg cccactgcca gccaccgtta ttggctgcca aatcaccatc aatcagctgc
    12121 gacatgaaat atcgctcacc ttcgcgccag tcgatcaata aatctttcac caaaaaactg
    12181 gctgtaatca tccgtagccg gttatgcatc cagccggtgc tgttaagctg acgcatggca
    12241 gcatcaacaa tcgggtatcc cgttttgcct tcctgccagg cctttaaatg cgcggaattg
    12301 ctctgccact gcacgcggtc ggtccagaca ataaacggac gatgtttaca cagcgagggg
    12361 taatacgtta tcagatgacg gtaaaactcg cgccagatca gctcattaag ccagatacta
    12421 ccggccccac cgtccagcgc ctgcggctgt tccgccaaca ggcgatgcaa acactggcga
    12481 ggcgataacc cgcccgttgc caggctggcg gacaaacggc tggtgccttc taccgcaggg
    12541 aaatctcgct gttgctcata ctctccggca ccgttctggc aaaactggcg taattgcgca
    12601 atcgccgctt tctcttccac cgggaaatgc gcagtatcga aaggctgacg aggataattc
    12661 agcgtaatgg atggcgcggg atctatcgat ccgctactac gtacttttgg cgcagcgacg
    12721 cactccggca tcccttctcg cagccgttta agccaggcgt ttttaaatgg cgtaaagact
    12781 ttgtacatct catgattgcc agtcatcacc gcccccggcg gcaggatcac gccgtcgtca
    12841 aatccttcac acaccacgtt acgcagcgcc ctctcagcct gcacatcccg cgcccgctca
    12901 ttcacctcat actgatagtt ataaaacaga tgggtaacac gattttccgc gcacacctgt
    12961 ttaactattt cgacgctggc ggcaaagtca tccacttcat gaaacagcaa aggaatgcct
    13021 ttttccgcca gcgcgatttg tagcccgttc agttgtgcat tgataaattc agcctgacgc
    13081 ggcgacatat tatgcgccgc ccactggcgt ggtgtagcga tatacaacgc cagcacgctt
    13141 gcagacgaat tgcggcaggc ggcagccagc gcgagattat catgctgacg taaatcctgg
    13201 cgaaaccaga ccagatgggt agtcataaaa ctcctgaatg gcgcaagtta atcacacgcg
    13261 gccaaagttc gaaataacgc tgccctgaaa gccaggcgtc aggataaagc gccatatagt
    13321 gtttgatacg catcagcggc gcaagaagcg gttgctcgcc accgcgataa ctgtcgataa
    13381 gcgccgccag ctgctggcgt tctgtggaat caatatgagg gcgaaaataa ccctgaacgt
    13441 gcatcaaaac attggtgtgg tcacgtcggt tggcggggtg agaaagcaaa acaactacgc
    13501 gttggcggta ctggttgtaa tagtcatcca gattttgcca ctggtgaata tcagccacga
    13561 acgggccaag tttgcggtac tcgggctggg aatgggccag aaagacgagt ttatagcggc
    13621 tgtgataatc gttgagtgcc ccgcgcgtta agccatgttc tttcagtcga tgtaactcat
    13681 aaagcgcgaa gatacggatt agagtgcttt catcaaatcg ttgctgattc attactgctc
    13741 ctgagatatt aacaggagta agtgtagacg ttggcaaatg atgtttgtga agttaatcct
    13801 gttgtagcag ggcaaaatta cgaaagcacg ctccccgcaa ggactgacgc cagatatttt
    13861 ctgtccatgg ctgccctttc cattcaaacg tgaaatcaat aagttcatca gggacatcgc
    13921 aaacaatatg aagacggatt tcttctctgc cgtgactctt ttcactcatt gcgtaactgc
    13981 catgctctgg attatttaat tctttcagcg gaaatttttc ctcgaaggct ttatttagcc
    14041 gcccctggt
//
LOCUS       DAZKGF010000129        13864 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZKGF010000129 DAZKGF010000000
VERSION     DAZKGF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.129"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..882)
                     /gene="yhaJ"
                     /locus_tag="ACHOQV_003658"
     CDS             complement(<1..882)
                     /gene="yhaJ"
                     /locus_tag="ACHOQV_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFP9631956.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKL"
     gene            987..1688
                     /locus_tag="ACHOQV_003659"
     CDS             987..1688
                     /locus_tag="ACHOQV_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFP9631957.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTVGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1711..1875
                     /gene="yhaL"
                     /locus_tag="ACHOQV_003660"
     CDS             1711..1875
                     /gene="yhaL"
                     /locus_tag="ACHOQV_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFP9631958.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2007..3317)
                     /gene="cyuA"
                     /locus_tag="ACHOQV_003661"
     CDS             complement(2007..3317)
                     /gene="cyuA"
                     /locus_tag="ACHOQV_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFP9631959.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYEIISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3345..4676)
                     /locus_tag="ACHOQV_003662"
     CDS             complement(3345..4676)
                     /locus_tag="ACHOQV_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9631960.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGVLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4951..6315)
                     /gene="tdcG"
                     /locus_tag="ACHOQV_003663"
     CDS             complement(4951..6315)
                     /gene="tdcG"
                     /locus_tag="ACHOQV_003663"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP9631961.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6387..6776)
                     /locus_tag="ACHOQV_003664"
     CDS             complement(6387..6776)
                     /locus_tag="ACHOQV_003664"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFP9631962.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6790..9084)
                     /gene="tdcE"
                     /locus_tag="ACHOQV_003665"
     CDS             complement(6790..9084)
                     /gene="tdcE"
                     /locus_tag="ACHOQV_003665"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFP9631963.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9118..10326)
                     /gene="tdcD"
                     /locus_tag="ACHOQV_003666"
     CDS             complement(9118..10326)
                     /gene="tdcD"
                     /locus_tag="ACHOQV_003666"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFP9631964.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10352..11683)
                     /gene="tdcC"
                     /locus_tag="ACHOQV_003667"
     CDS             complement(10352..11683)
                     /gene="tdcC"
                     /locus_tag="ACHOQV_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFP9631965.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11705..12694)
                     /gene="tdcB"
                     /locus_tag="ACHOQV_003668"
     CDS             complement(11705..12694)
                     /gene="tdcB"
                     /locus_tag="ACHOQV_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFP9631966.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12793..13731)
                     /gene="tdcA"
                     /locus_tag="ACHOQV_003669"
     CDS             complement(12793..13731)
                     /gene="tdcA"
                     /locus_tag="ACHOQV_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFP9631967.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
BASE COUNT         3594 a         3746 c         3248 g         3276 t
ORIGIN      
        1 aagtttggga atttcccgca gacaccagga tttcgcctcc cccatactgt cacgacgcca
       61 ggccataata atatcgatct cgctggtcga ttccgggctg acgacacgca accgcccttc
      121 cgcgatgtct ttttcgacca tcggatacgg catcgtcgcc acgccaagcc ccgccagtaa
      181 cgcctgacgt ttatcttcaa tcgtgctcac cgttaaacgc ggctgtttgt ccagtagctg
      241 tacggtcaat accgggcgct cacgagcggt atccgccacc gcaattccac gatatttcac
      301 gcgcgtcact tcagataacg gttccggctc ctgatgaatc gggtgatcag gcgcggcaac
      361 gtagacgttc attaacgtat agagcttgcg cgagttgatc tccgacgagg agcgaaaatg
      421 catatccggc gcgataacaa tatccgcccg cccctgctcc agccgttccc atgcccccgc
      481 cagcacttcg gtgatgattg ccagttgggt attcgctttt gccgccagtt tgtcaattaa
      541 cgggaaaaag gcaggtgtcg gcaccagcgc ttcggtcaca atggtgagat gcgtttccca
      601 accgcgcgcg agagcttccg catcggtagt cagtttatct gcggcttcca gcaaaacgcg
      661 tcctcgctcc agcaacatcc tcccgacatt ggtgaatttg gtgcgatggc ccgagcggtc
      721 aaacagcacc acatccagct cttcttccag tttttgcatg gtgtagctaa gtgcggaagg
      781 cacgcgtccc agctcatccg ccgccgccgc aaaactgccc cggcgatcga tcgcatccat
      841 aacccgtagt gcttccagcg ttaatgccct ttctttggcc atttcgttct cattcaggaa
      901 atttgaacat accgggcaga atatctggct aacaatgtag cgtccagccc cttaccataa
      961 aaggaagtaa agagaggtca agaattatga ttactacccg aactgccagg cagtgtggac
     1021 aagcagacta cggatggttg caggcccggt atactttttc ctttggacac tacttcgacc
     1081 cgaaattgtt aggctatgcc tccctgcgcg tgcttaacca ggaagtgctg gccccaggtg
     1141 ccgcctttca gccgcgaacc tatcccaaag tcgatatttt aaatgtgatt ctggatggag
     1201 aagcagagta tcgcgacagc gaaggcaatc atgttcaggc cagcgctggt gaggcgttgc
     1261 tgctctctac ccagccgggt gtcagctata gcgaacacaa tctcagcaaa gacaaaccgt
     1321 taacgcgaat gcagctttgg ctggatgcct gcccgcagcg agagaatccg ctgattcaaa
     1381 aactggcgct taatatgggc aagcagcaat taatcgcctc gccagagggc acagtgggta
     1441 gcctgcaact gcgccagcaa gtgtggctgc accatatcgt gctcgacaaa ggcgaaagtg
     1501 cgaatttcca gttgcatgga ccacgcgcgt atttgcaatc gattcacggg aaatttcatg
     1561 cgcttacgca tcatgaagag aaagcggcgc taacctgcgg tgatggggcg tttattcgtg
     1621 acgaggctaa cattacgctg gttgccgatt ccccactgcg cgctttgctg atagatttgc
     1681 ctgtctagtt gtttttacag ggagatgatg atgagtaaaa aatcggccaa aaagcgccag
     1741 ccagtgaagc ccgtggtggc gaaagaacct gctcgtaccg ccaaaaattt tggctatgaa
     1801 gagatgttga gcgaactgga agccatcgtc gcggatgctg aaacgcgttt agccgaggat
     1861 gaagctaccg cgtaaagaca ggtgccggat gcggcgtaaa cgccttatcc ggcctacaaa
     1921 acccaacaac atcaacaaat tgtgcaattc gtaggccaga taagacgcgc cagcatcgca
     1981 tccggcacga tacccaaaac tggcgtttat ctggccttgc tcgccataat ctcgataatc
     2041 tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc aatcgactgc
     2101 tcaacatcat gcgccacgat cccctcattg ccggtcacgg cggtatcatc cagcgccatt
     2161 aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca gctgttcgac
     2221 gctccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc catcgaaatg
     2281 atttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat tgctgcggtc
     2341 gttgcggcac acagcgcaga caaacgcggt aactggttat ggatgtaaat tgcgctcaaa
     2401 tgcgaaagca tcagcgcacg cgccagccgt tcatcatcgg ccccgaagtg ttctgctacc
     2461 accaccacgg gcattgttgc agtgatcccc tggttacccg agccggagtt actcattgcc
     2521 ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat cacaatggat
     2581 gaagagagat ctttcgccag caaaccgcgt gcacactgtt tttccagcgt cgcgccaata
     2641 tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag cttcgcggaa
     2701 tcgagaataa agcggatcgc cgcaaacggg acttcattga cgaacttcag gatctcagcc
     2761 agcgtcgttc ttgaaagcac cgtcagcggc gactcttgct cgccctctgt cacacacgcc
     2821 tgctgggtaa acaccacacc attgtgcgtc tcaatatgca caatgttggt atgcccgccg
     2881 acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga gaagaggatt
     2941 tcatcgcaag gttcctggat cttaactgag actttccccg ccgccagcag tgctttggca
     3001 tcggcaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt agcatttcca
     3061 cctaacgctc ccagcgccgc cgcaattggc agccccacca ttcccgtgcc gggaacggtg
     3121 acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg ttcaaccgga
     3181 ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg ttcagtacat
     3241 cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca taacggattt
     3301 aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga aaatgccagg
     3361 aacggagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga catccctttg
     3421 tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca ccctaccatg
     3481 ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gtgcgttcag tacgatggcg
     3541 ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacgagatt ttcgttaatc
     3601 ttctcggcag gcatcttgcg acgcaggatg ttcattacga tcccttgcgt tgcttcgcga
     3661 aagcctaaat agacgccaaa gaatgcagtc attacagcaa agatattgag aatgacgctg
     3721 accactttca cccacgctgc gccatcaccg ctaataaact gtgcggcaat cgccaacgcg
     3781 gaaatattct gctcgtaggc ttttaccgct tcgtcatgtc ccatcgccag cgtgaacgac
     3841 acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat tgcccgcaat
     3901 gctttatgcc gcgccacttc aattgatttt tcccgcgagc gataagagat caccatcggg
     3961 cttaacgtct ggataaacag aatcgacgtc agggtaaacg gcagcgtaat aatggcgttt
     4021 ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca cattcccacc
     4081 atcgacacgc ccagcgccgc gaccaccagc agcttggtca gcaccatgcc ggtcgaaatt
     4141 ttaaataaca atttctcgcc gcgtgaggag atcgccacca gaatgcaaat cagtaccaga
     4201 ccataaaagg gactgtctga cagcacccct tccgtcacgc cgaaggtatg caggtaggaa
     4261 gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag cattacgaaa
     4321 tagagcgcac ctaacaggat gccccagttt ttacctaaat aaccgctgat gacgctcggg
     4381 taatctttac actccggtga ttctgccagc gtattaataa acaaccgctg aaacagatac
     4441 attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat caaaccgacc
     4501 tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat aatcacccag
     4561 ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact cattccagca
     4621 cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcaat ttccataatt
     4681 tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa ggcgatatgc
     4741 ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg aggtcagaga
     4801 aaatcttcac aatcaagact ttgaaataac ggagatggat aagaattttc tactaaatta
     4861 atcgcaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg tgcacatttg
     4921 tgcacccaag gatgaaagct gacagcaatg tcagccacag accactttaa tggccagtcc
     4981 tccgcgtgac gtttcgcggt atttatcgtt catatctttg ccggtttcat acatcgtctc
     5041 gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca tccgcgcggc
     5101 gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt gtacctgtcc
     5161 ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg ccgcattgca
     5221 tacctgcgcc ggactgccgc ccaggagttc agttaaccct gccgccgcca ttgaacaggc
     5281 cacgccaatc tccccctgac agccgacctc cgcaccagag atggaggcgt tcattttata
     5341 cagcgcgcca atagcccccg ctgcaagaaa atagcgggca attgaccgct cgtttaccgg
     5401 acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg caccgttagt
     5461 cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca gcgcgtacat
     5521 attgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctgg aaaccagttg
     5581 acgacgcagc gctacggcac gacgcggcac attgagcgga ccaggcagca cgccttcagt
     5641 gttcatcccg cgttcaatac cgtcatgcat cacttgccag atacgggcaa accctgcgtc
     5701 aatttccgct ttactgcgca gcgccagctc gttgtgcatc atcaggccag atatagacag
     5761 gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt aaggtacggg
     5821 tgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc cgccgccgac
     5881 agagtaatag gttttgctta atagctcttc ctgtcccttc caggcagtga tccgcattcc
     5941 gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta caggaaaatc
     6001 aacaatatgc gcgccagaag ccaccggcaa ccgcccgctg cgcgtcacta actctataaa
     6061 tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc ccatgatgat
     6121 ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccgtaca gatcgaccac
     6181 aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa aactttttcc
     6241 ggcattcatt ggccccacag tatgcgaact ggagggacca atcccaattt tgaaaatatc
     6301 gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga gcggaacgac
     6361 cttgcgaccg tcccgctcac gaggccttac gcactacgta ctgcgatggc ttcaatttcc
     6421 aacttcacat ctttcggcaa acgcgcgacc tgcacacagc tccgggtcgg gtaggtcgcc
     6481 tgatgttcat cgaagaactg cttatagact tcattgatgg tggcaaaatc attcagatcg
     6541 gtgatgaaca cggtcatctt gatgatatcg cccacactca gcccggcagc aaccacgatc
     6601 gctttgacgt tttcgaggct taaacgcgcc tgatcttgca catcagccgg gatctcaccg
     6661 gtctgtgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa atcaacgccc
     6721 tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt tttcatacat
     6781 cctccggcgt cagagcgcct gggtaaaggt acgtgaaata acatcctgtt gctgttcacg
     6841 ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt taggatattt
     6901 ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga cgttgaggtg
     6961 ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc cgacaaggtt
     7021 ggttttacgt actggatctt ctttgcccag cgccgcaggg acgatggaga aggtgtacga
     7081 gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg aggccacggc
     7141 gcctttgcga tcacgaccgt gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg
     7201 gcgaccgtcc ggcgtgttcc cggttttctg accgtacacc acgttagagg tgatagtcag
     7261 aatcgattga gtcggcacag cattgcgata ggttggcaga gctttaattt tcttcataaa
     7321 gcgttcaacc agatcgcagg caatgctgtc tacgcgctcg tcgttgttgc catactgcgg
     7381 atattcgccg tcgatttcga aatccaccgc caggccgttt tcgtcacgga ttggtttcac
     7441 acgagcatat ttgatggcag acagggagtc cgttgccacc gacaggcccg cgatgccgca
     7501 cgccatggtg cgatagacat cacgatcgtg tagcgccatc agcgaggctt catagctgta
     7561 cttgtcgtgc atgtagtgga tgatattcag cgcgctgatg tactgtaccg ccagccagtc
     7621 catgaagtgg tcgaggctat ccatcacttt gtcgtagtcc agcacgtcat ccatcaacgg
     7681 agccgttttc gggccaacct gaatcttcag cttctcgtcc attccgccgt tgattgcgta
     7741 cagtagcgtt ttcgccaggt tagcgcgcgc accgaagaac tgcatctgct taccaatcac
     7801 cattgggctg acgcaacagg caatcgcgta atcgtcgcta ttgaagtcag tacgcatcag
     7861 atcgtcgttt tcatactgca aagaggatgt gacgatagac acctgcgcgg catatttttt
     7921 gaaggcaatc ggcaaggctt ccgaccacag aatagtcagg ttaggttccg gtgcaggccc
     7981 catagtgtgc agggtgtgta aatagcggaa ggagtttttg gtcaccaggg ttcgaccgtc
     8041 cagccccatc ccgccgatca cttccgtcgc ccagatcggg tcgccggaga acagcgaatc
     8101 aaattccggc gtacgcaaga agcgcaccat acggatcttc atgatgaagt ggtcgatcag
     8161 ttcctgtgcc tgctgctcat tgagtacgcc tgctttaaag tcgcgctcaa tgtagatatc
     8221 gaggaacgat gccgtgcgcc ccagcgacat cgcgccgcca ttttgcgatt tcactgccgc
     8281 caggtaagcg aagtagagcc actgcaccgc ttcctgtgca ttctgcgccg gacgggagat
     8341 gtcaaaaccg tatttcgccg ccatttcttg aatctgcaac agcgcacgac gatgttccgc
     8401 cagctcctca cgcagacgga tcgtagcttc gagatcttcg cctttttcca gacgggactg
     8461 gagatcggca aactgcagtt cgcgttcacg taccagataa ctgatgccgt aaagcgccac
     8521 gcggcggtag tcaccgatga tacgcccacg accataacca tctggtaaac cggtcagtac
     8581 accagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc cctggttatg
     8641 ggttttacgc agatcggtaa agatgtattc aaattcgcta tccatttcac gaccatacgc
     8701 gtggaatgaa cttctaatca tattaatgcc gccgaacgga tgcagcgcac gctttaatgg
     8761 cgcatccgtt tgcagaccca ctattttttc cagcggctgg tcaatatatc ctgcatcatg
     8821 agcggtaatt gtggtggcaa tattggtatc gaaatccaat ggcgcgtgag tcgcattttc
     8881 gatacggatg ccttccatca ctttttccca caattccgtg gtggcaggcg tcgcttcggc
     8941 gagaaaagat tcatcacctt catacggtgt atagttatgt tgaataaaat cgcggacatt
     9001 aatttcgttt ttccagtccg tacctttaaa gccaagccat gcgtcggcgt acagcttatc
     9061 gctggtatca atatctacct tcatgaaaaa taatctctct acaatacttc aactaaatta
     9121 tgcaaattct gcgggcgcgt tcactttgcc taaatgaatg gcatccaaag caatcatttt
     9181 ttcttcgtta gtcggaatta cggcgcaaat gacacgcgca ttttcactgg aaacaattcg
     9241 ctctccaaaa gagttagagc gattattcat ttctgtatca atctctacgc ctaatacagc
     9301 taaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc cgccggtgaa
     9361 tataattcca tccaggcgat gtaatgaagc agcgtgtccg gcaatatgac gggcaattcg
     9421 atgaacaaag gttttaattg ccaattgcgc gcgttcgtga ccttcatgcc aggctttttc
     9481 cagaacacgt aaatccgaag aaaggccgga aatacctaat aatcccgact ctttattcac
     9541 gacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgacccagg acatcgcccc
     9601 gaagtcgaca tcaccactgc gggtacccat catcaagcct tccagcggcg tcattcccat
     9661 tgaggtatca acactctgac cgttgcgaac tgcgcagatt gacgcgccat tgccaagatg
     9721 cgccacaacc aggccggagt catcttccgc cagattcagc aaagaatgcg cgcgctggga
     9781 aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact cttcataata
     9841 tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga aactggtatc
     9901 aaataccgct acctgagtta cgcccggaaa taattgctgc gccgattcaa taccacttaa
     9961 attggcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat caatgacttc
    10021 atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc ggtggccaat
    10081 taaggccaca ctgtcattta aattccgttt ttccagttca aatgcaattg ccttcaatgc
    10141 accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg ataagaatgc
    10201 attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt cgctggcatt
    10261 gagtacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa ccggaaattc
    10321 attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg atgttcagga
    10381 tagtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg tatttcgcca
    10441 gagacggcgc tttacgaatg gcatacatcg gcaacaggca tagcagtgat gcgataatcg
    10501 gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca acaacccagg
    10561 tggagcccat gatgaagatc atgctgagag tattcagttt acccagcgac actttggttt
    10621 tgtcaccttt ataaccgaac ttcagaatca ggccattcaa gccttccagc gtccccagat
    10681 agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc gcatattcca
    10741 gtgtaatcgc gaacgttgtt ttggtaccgg tcatggacgc aaagtggtta gccagataag
    10801 aaagcactgg aatattctgc gctttggctt ccgccatgtt ggccggagac agcgtaaaca
    10861 ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca cgagaaatga
    10921 tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac tcttcacgtt
    10981 tagaaaccac gaaggaagag acgattggcg agaagttaaa ggagaaaacc atgatggaaa
    11041 tccccagcca gacagtgatc aggataccgt catgaccggt taacgacagc gaaccgaggt
    11101 caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa atcagcacca
    11161 ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga tccttaccaa
    11221 accagatgac gaaagccatc agcagcaaca ggaacagcgc cacaaagccg cgattcagcg
    11281 gtgcaaagcc gagctggttt tcccagaacg tcataaaggt attggtaata gtaacgccat
    11341 aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc acgccgccag
    11401 ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta gagccagaaa
    11461 gacacagacg cgccagtgcc cggtggcaat aaaacgcgat ggggtatgct aataccaaca
    11521 tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac agcaccccgg
    11581 cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta cgccaggacg
    11641 attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat cctcaacgaa
    11701 ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg atattgccgc
    11761 cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta ccgcttaata
    11821 atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc tgaattaagg
    11881 caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg actaattcac
    11941 gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc gccagggtgc
    12001 cggtagttcg gtgcgtagtt atttctccgg agtggaaaga agccgccatg ccgtgaacgt
    12061 tttctgactg tacgccaata acacgaatag tcgggttaat tgatttaatc gctaccgcaa
    12121 taccagcaat taaaccgcca ccgccaattg gcacaatcac attatcgaca tcatagagat
    12181 cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga tcatcgtaag
    12241 gtgggataaa aatacggcct tccatttcga caatttcgct cactttagca atagtgtcgt
    12301 tgaagttatc accatgcaaa acgacttctg cggagtagtc gcacgttgcc gcgactttgg
    12361 atttcggcgc accttttggc atcaccactt taccgtcaat acccagcatc gcacaggaga
    12421 gcgaaacccc ttgcgcatgg ttgcccgcag agcaggccac tacaccttta cgtttttccg
    12481 catcagtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa ccggtacgct
    12541 gcatgttttc gaatttgagg aatatttcac ctttgcaacg ttcactaaaa tagttggagc
    12601 gaggcatacc tgttttataa attcgcccag ccagtcgttg ttttgcttca atgatgtcat
    12661 caatagcaac cgggagatcg tatgtaatat gcattataaa acctcttagc ctgaattaaa
    12721 tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt atgtttaaat
    12781 taaaacaaat aactaatcaa cttcaattaa ttgccttcgt ctacacccat tataagatga
    12841 atactctttg gctaattcca ccaaaaccga tgctgctttt ttaatacgat aatttttcga
    12901 ccataccgcg gcatattgtg ccacaggtaa tgtttcttca accggaatag taataaattg
    12961 attagaacca aaaggtgatg tcatatcaca aggaattaca gttaagaaat cagcattgag
    13021 aacaagatta taaattgtca cgactgagtc ggttttaacg atgttttcaa tactgatgcc
    13081 atttctttgt aacgtagtaa gtagttcgct gtagtacccc atattggttt gtggcaacac
    13141 ccactgttcg ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc gggacttact
    13201 ggctaccagc acaaactcgg actcgaacag cggctcaaca tgtaaatcct gaagcttcat
    13261 ttctgcgctt aacgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg ccggtaagaa
    13321 cgaagacagt tgcgcttcat acatagaaac ctgcgctttc gggaacacct ctttgaactt
    13381 gttgatcatc cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa atgagacttc
    13441 caccaccgcc tcagaagaca taccgcttat ctcattaacc atatttttca tttcacgggt
    13501 aatggattcg gaacgggaga gtaacaattg accggcaggt gttaatgtta caccggtatt
    13561 tttccgcacc actaactcca caccaaaata atcttcaata tcgttaatga ttttactgac
    13621 ggccggttga gttaacccta actcttttgc agccgagccg atagaaccac ttctaataac
    13681 ttcctgaaag actaccaggt gctgcgtttt cggaagaaga atagtgctca taacgaccta
    13741 cgttaattac ctcattgacg gcatgaagtg tatcaaaatg aaatgaacag gatatgtgcg
    13801 accactcaca aattaacttt caatactttc cagggtatcg ttattacaat taaatatctt
    13861 tata
//
LOCUS       DAZKGF010000130        13856 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZKGF010000130 DAZKGF010000000
VERSION     DAZKGF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.130"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..1364
                     /gene="chbC"
                     /locus_tag="ACHOQV_003670"
     CDS             6..1364
                     /gene="chbC"
                     /locus_tag="ACHOQV_003670"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFP9631968.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWTLNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            1415..1765
                     /gene="chbA"
                     /locus_tag="ACHOQV_003671"
     CDS             1415..1765
                     /gene="chbA"
                     /locus_tag="ACHOQV_003671"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFP9631969.1"
                     /translation="MMDLDNIPDTQTEVEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKTMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1773..2615
                     /gene="chbR"
                     /locus_tag="ACHOQV_003672"
     CDS             1773..2615
                     /gene="chbR"
                     /locus_tag="ACHOQV_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFP9631970.1"
                     /translation="MMQPVINALEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            2719..4071
                     /gene="celF"
                     /locus_tag="ACHOQV_003673"
                     /gene_synonym="chbF"
     CDS             2719..4071
                     /gene="celF"
                     /locus_tag="ACHOQV_003673"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9631971.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSMNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            4084..4842
                     /gene="chbG"
                     /locus_tag="ACHOQV_003674"
     CDS             4084..4842
                     /gene="chbG"
                     /locus_tag="ACHOQV_003674"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFP9631972.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSLDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGCYRDV"
     gene            complement(5100..7361)
                     /gene="katE"
                     /locus_tag="ACHOQV_003675"
     CDS             complement(5100..7361)
                     /gene="katE"
                     /locus_tag="ACHOQV_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFP9631973.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            7544..7807
                     /gene="cedA"
                     /locus_tag="ACHOQV_003676"
     CDS             7544..7807
                     /gene="cedA"
                     /locus_tag="ACHOQV_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFP9631974.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            complement(7854..9245)
                     /gene="tcyP"
                     /locus_tag="ACHOQV_003677"
     CDS             complement(7854..9245)
                     /gene="tcyP"
                     /locus_tag="ACHOQV_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFP9631975.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(9378..9968)
                     /gene="ydjM"
                     /locus_tag="ACHOQV_003678"
     CDS             complement(9378..9968)
                     /gene="ydjM"
                     /locus_tag="ACHOQV_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9631976.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(10132..10800)
                     /gene="hxpB"
                     /locus_tag="ACHOQV_003679"
     CDS             complement(10132..10800)
                     /gene="hxpB"
                     /locus_tag="ACHOQV_003679"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFP9631977.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYIDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            10947..11483
                     /gene="yniB"
                     /locus_tag="ACHOQV_003680"
     CDS             10947..11483
                     /gene="yniB"
                     /locus_tag="ACHOQV_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFP9631978.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFFLIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(11524..12384)
                     /gene="yniA"
                     /locus_tag="ACHOQV_003681"
     CDS             complement(11524..12384)
                     /gene="yniA"
                     /locus_tag="ACHOQV_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP9631979.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(12490..12780)
                     /gene="ghoS"
                     /locus_tag="ACHOQV_003682"
     CDS             complement(12490..12780)
                     /gene="ghoS"
                     /locus_tag="ACHOQV_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP9631980.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEIAITTWEEWNSNRK"
     gene            complement(12881..13810)
                     /gene="pfkB"
                     /locus_tag="ACHOQV_003683"
     CDS             complement(12881..13810)
                     /gene="pfkB"
                     /locus_tag="ACHOQV_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFP9631981.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPVGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPLGVKLEKLTQLISAAQNQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         3473 a         3282 c         3543 g         3558 t
ORIGIN      
        1 tttctatgag taatgttatt gcatcgcttg aaaaggtact cctccctttt gcagttaaaa
       61 taggaaagca gccacacgtt aatgcaatca aaaatggctt tattcgctta atgccgttaa
      121 cccttgcggg ggccatgttt gtattaatta acaacgtttt tctaagcttt ggggaggggt
      181 cgttttttta ttccttaggt attcgcctgg atgcctcaac cattgaaaca cttaatggtc
      241 tgaaaggtat tggcggcaac gtatataacg gaacattagg gataatgtct ttaatggcac
      301 cgttctttat tggcatggcg ctggcagaag agcgtaaagt cgatgcgctg gcggccgggt
      361 tgttatccgt tgcagcattt atgaccgtca ccccatatag tgtcggtgag gcctatgcgg
      421 ttggcgcaaa ctggttaggt ggggcgaata tcatctccgg gattattatt ggcctggtgg
      481 tggcagaaat gtttaccttt attgtccgcc gcaattgggt cattaaattg cccgatagcg
      541 tacctgcttc agtatcgcgt tccttctcgg ctttaattcc cggctttatt attctttccg
      601 tgatggggat tattgcctgg acgttgaata cctggggcac caacttccat cagattatta
      661 tggataccat ctcaacccca ctggcatcgt tgggtagcgt ggtgggctgg gcctatgtga
      721 tctttgttcc actgctctgg ttcttcggta ttcatggtgc gctggcgctg accgcactgg
      781 acaacggcat tatgacgcct tgggcgctgg aaaatatcgc gacctatcag caatatggtt
      841 ccgtcgaagc ggcgctggca gccggtaaga ccttccatat ctgggccaag ccgatgctgg
      901 actcctttat tttccttggg ggcagtggtg cgactttagg tctgatcctg gctatcttta
      961 tcgcctctcg ccgtgctgat tatcgtcagg tggcaaaact ggcgctgccg tccggcatct
     1021 tccagattaa cgaaccgatt ctgtttggtc tgccaattat catgaacccg gtgatgttta
     1081 tcccgtttgt actggtacaa ccgattctgg cggcaatcac cctcgcagcg tactacatgg
     1141 gcattattcc tccggtgacc aatattgcac cgtggaccat gccaaccggt ctgggagcct
     1201 tctttaacac caacggtagc gtcgccgcat tgctggtcgc actcttcaac cttggtatcg
     1261 caacgttaat ttatctgccc tttgttgtgg tggctaacaa agcacaaaac gcgattgata
     1321 aagaagagag cgaagaagat atcgctaacg ccctgaaatt ttaatcgatg ccggtacggg
     1381 taatcgtgcc ggtaagaata gagaggaacg atgtatgatg gatctcgata atattcccga
     1441 tacgcaaacg gaagttgaag agctggaaga agtggtgatg gggcttatca tcaactccgg
     1501 acaagcgcgc agcctggcgt atgcggcact gaaacaggcg aagcagggcg attttgccgc
     1561 agcaaaaacc atgatggatc agtcacggat ggcattgaat gaagcgcatc tggtacagac
     1621 gaaactgatt gaaggcgatg cgggcgaagg taagatgaaa gtgagtctgg tgctggtcca
     1681 cgctcaggat catttaatga cgtccatgct tgcgcgtgaa ctgattactg aattaattga
     1741 gcttcatgaa aaactgaagg cataaggagt cgatgatgca gccagtgatt aacgcgctgg
     1801 aaattgccac tgcccgagaa cagcagttgt ttaatggcaa aaatttccat gtgtttatct
     1861 acaataaaac tgagagtatc agcggactgc atcagcacga ctattatgaa tttactctgg
     1921 tattaaccgg gcgttatttc caggagatta acggtaagcg cgtgttactg gaacggggcg
     1981 attttgtttt tattccgtta ggttcgcacc atcaaagttt ttatgaattt ggtgccacgc
     2041 gtatattgaa cgttgggatc agtaaacgct tttttgagca gcattacctg ccgttgttgc
     2101 cttattgctt tgtcgcttcg caggtatacc ggaccaataa cgcttttctc acctatgtgg
     2161 aaacagtgat ttcttcattg aatttccgcg aaacagggct ggaagagttt gttgagatgg
     2221 ttacttttta tgtcattaac cgtttacgtc attaccgcga agaacaggtg attgatgata
     2281 taccgcagtg gctgaaaagt acggtagaaa agatgcatga taaagagcag tttagtgaat
     2341 cggcgctgga gaatatggtg acgttgtcag ccaaatcaca ggaatatttg acgcgagcga
     2401 ctcaacgata ctatggtaaa acgccaatgc agattattaa tgaaatccgt attaattttg
     2461 ccaaaaaaca actggaaatg accaactatt cagtgacgga tattgcgttt gaggccggtt
     2521 atagtagccc gagcttgttt attaaaacgt ttaagaaatt aacatccttt acgcctaaga
     2581 gctatcgtaa gaaattgact gaatttaatc agtaagtcgt tatacctgac aattcacata
     2641 tctgtcctgt tgctggatta tttatgtccg gggggcagat atgccagata tcagtattct
     2701 gtactgaagg gagaaattat gagccagaaa ttaaaagtcg tcactattgg tggcgggagc
     2761 agctataccc cggagttact ggaaggattt attaagcgtt atcacgaatt gccggtcagc
     2821 gaattatggc tggtggatgt cgaaggtggt aaagcgaaac tggatattat ttttgatctc
     2881 tgccaacgga tgattgataa cgctggcgtc ccgatgaagc tttataaaac gctggatcgc
     2941 cgcgaagcat tgaaagatgc tgatttcgtt actacccaac tgcgcgttgg ccaattaccg
     3001 gcgcgcgaac tggatgaacg tattccatta agtcatggtt atcttggtca ggaaactaac
     3061 ggtgcgggcg gtctgtttaa aggtctgcgt accattccgg tgatttttga catcgtaaaa
     3121 gatgtcgaag aactatgtcc gaatgcatgg gtgattaact tcactaaccc ggcgggaatg
     3181 gtcactgaag ccgtttatcg tcataccgga tttaaacgct ttatcggcgt gtgtaatatt
     3241 ccgatcggca tgaagatgtt tattcgcgat gttctgatgc tgaaagacag cgatgattta
     3301 tctatcgatc tgttcggcct caaccatatg gtgttcatta aggatgtgct ggtaaatggc
     3361 aaatcacgct ttgccgaatt gcttgatggt gtggcatccg ggcagttaaa agcatctggc
     3421 gttaaaaata ttttcgatct gccatttagc gaaggcttaa ttcgttctat gaatctgttg
     3481 ccgtgttctt atttgcttta ttacttcaag caaaaagaga tgctggctat tgaaatgggc
     3541 gaatactaca aaggcggcgc acgagcgcag gtagtacaga aagtcgagaa acaacttttt
     3601 gagctatata aaaacccgga gttgaaagtt aagccgaaag aactggaaca gcgcggtggg
     3661 gcttattact ctgatgcagc gtgcgaagtg atcaacgcta tctacaacga caagcaagct
     3721 gaacattacg ttaatatccc gcatcatggg catattgata atattccggc agactgggcg
     3781 gtagaaatga cctgtacgct ggggcgcgat ggcgcgacgc cacatccgcg cattacgcat
     3841 ttcgatgata aagtgatggg gctgattcac accattaaag gcttcgagat tgctgccagc
     3901 aacgccgcac ttagcggaga gtttaacgat gtgttactgg ccctaaacct tagtccgttg
     3961 gtgcattccg atcgcgatgc tgagctgctg gcacgcgaga tgattctggc gcacgagaaa
     4021 tggttgccaa attttgccga ctgcatcgca gagcttaaaa aagcacatta accgaggctg
     4081 attatggaac gcttactgat tgttaatgcc gatgattttg gcttaagcaa aggtcagaac
     4141 tacggcatta tcgaggcctg tcgcaatggg attgtcacat cgacgacggc gctggtgaat
     4201 gggcaggcta ttgaccatgc ggtgcagttg agtcttgatg aaccgagtct ggccataggg
     4261 atgcactttg tccttactat gggcaagcca ctgacagcta tgccggggtt aactcgcgat
     4321 ggtgtgctgg gaaaatggat ctggcagttg gcagaagaag atgctttacc gctggaagaa
     4381 attactcagg agcttgccag tcagtatttg cgtttcattg agttatttgg acgcaaacct
     4441 acgcatcttg atagccatca tcatgtgcat atgttcccgc agattttccc gattgtggca
     4501 aagtttgcgg ctgaagaggg gattgcgttg cgcatcgacc gtcagccgct atctaacgat
     4561 ggtgatttac cggctaatct gcgcagttcg cagggattca gtagcgcgtt ttatggtgaa
     4621 gagattagcg agacactgtt cctacaagtg cttgatgact ccagtcatcg cggcgagcgc
     4681 tctctggaag tgatgtgcca cccggcgttt atcgataata cgattcgcca gagtgcttat
     4741 tgcttcccgc ggttaacgga gctggatgtg ttgacatcag cgtcgttgaa aggtgctatc
     4801 gctcagcgtg gttatcggtt ggggtgttat cgggatgtgt aagtgaggat gtttgccaga
     4861 tgcggcgtga accttgcatc cggcgattaa ttcctacaaa ggcggcgtaa ctgcgccgct
     4921 tgagactgct gacaaacgca aaactgcctg atgcgcttcg cttatcaggc ctacgttttt
     4981 cctgcaatat attgaatttg cacagttttg taggccggat aaggcgttca cgccgcatcc
     5041 gacataaaca aagcgcacta tgtaaatcat ttgaggcggc gcaactgcgc cgcctcccat
     5101 caggcaggaa ttttgtcaat cttaggaata cgtgaccaca cgcggtgtgc tgccatcagc
     5161 gttaacagtt catccataaa actaccgtcg gcgctgtcag cttccacaat cccttcttca
     5221 ccctggtcag cgaccttgat tgttgcttta aacttgcgcg cgtctcctgc cagcgcaatc
     5281 ggtttaaggt gtttgtaggc ttccatcagg tagtagttgg catcgccgtt gtcagcgata
     5341 tccgcgatat tgccgcaagg gacaataacc gcatcgaccg tcagcgaagg tgcaccggca
     5401 aaggtagcgg ctataggcaa caccgtaccg tcatccgcag tcacttcacc cattcgggag
     5461 tagagcagtt tggcatgaac gcctttggcc ttcagcgcct tgagaatggc cagaaggtct
     5521 gccgatctca cttcatcatt aagtaaaatc gctaccacgc gacctttcac atcaccgtca
     5581 ggaatggcgt acaaacttaa ggatggatcc tttttcagac cgttgacgtc cggaggcggg
     5641 gtgatattca gctggtcgtc cgtcagctcg ataccgagat ttttcgccac cgcctgggcc
     5701 agagtgagat caatatgtgc cagctggtca acaacgcgct cacgaatata cggacgaacg
     5761 actttgctta actcaaaact gaaaccatcg acaatatggc gctgctcgaa tggcgtctga
     5821 cttagccaga acagacgcgg atgggaataa tattcgccaa acgatgggct gcgctcgcga
     5881 actttattgc cttccacgcg ctcctggtat gattcaaaac cgccgcgttt cggccccggc
     5941 ggtgtttcgc gcggccagtt atcgttgatc gagttcggtt cataattcgc cgggttagtg
     6001 tcgatcccca tacgatgcat gccgtcacgc tggaaattat ggtaagggca ggtcgggcgg
     6061 ttaatcggaa tctcatggaa attcggccca ccaagacgac tgatttgtgt atcggtatag
     6121 gagaacaaac gtccctgcaa caggggatcg ttggtgaagt ccagaccagg aacaatatga
     6181 cctggatgga atgctgcttg ttcgttttcg gcaaagaagt tatccggatt gcgattgagc
     6241 accatcttgc cgacacgctg aacgggcacc agttcttccg ggataagttt agttggatca
     6301 agaaggtcga agtcgaactt aaattcatct tcctcaggaa tcaactggaa gcccagttcg
     6361 tattccggaa aatcgcctgc ttcgatggct tcccacaact cgcggcggtg gaagtccggg
     6421 tcacgtccgg ttagtttttg tgcttcatcc caaacgagtg aggctttacc tgccagtggt
     6481 ttccagtgga aacgtacgaa cgttgccttc ccttcggcat taatcaggcg gaaggtgtga
     6541 ataccgaagc cttccatggt gcggtaactg cgcgggatcc cgcgatccga catcgcccac
     6601 atcacgttgt gcagagtttc aggttgcaga gaaacataat cccagaaagt atcgtgggcg
     6661 ctttgccctt gtggaattgc ccagtgcggt tctggtttta ccgcatgaac aaaatcgggg
     6721 aatttatgcg catcctggat aaagaagatt ggcgtgttat tgccaacgag gtcaaaaata
     6781 ccctcttcgg tatagaactt ggtggcaaag ccacggatat cacgcacggt atcggcagag
     6841 ccagcaccac cctgaacggt agagaaacgt acaaatactg gggtgatttt gttcggatct
     6901 gagaggaaat ctgctttggt gatatcgctt aagcttttat atggctggaa ataaccgtga
     6961 gctgctgatc cgcgtgcatg aacaatacgt tccgggatgc gctcatggtc aaagtgagtg
     7021 attttctcgc gcagaataaa atcttccagc agagttggac cacggctacc ggcacgcagt
     7081 gagttttgat cgtcggcgat gcgcacgccc tgattagtgg tcagcgcata attttcactg
     7141 cctttgcgta cgtcttccag agaattaagt ttttcgttac gcgtatcagg ggctttcagg
     7201 ctccctgggg cggtaggttg tgcaccaggc ggtgttggtt cagccgctgg acgatgagat
     7261 ccgtcttcag gtgccagtga gtccatcccc ggtttcgctt cgctggaatc gtgtagtggt
     7321 gactggtgct gatgtgggtt cttttcgtta tgttgcgaca ttgaactcgt ctccttaaat
     7381 tattactgaa agggccgctg ttttggtcaa aaaaccctct aactatagac catcagccag
     7441 atcccgcttc ggagactatt ctggaaacgg tgttatgaaa tacacgcaaa gcgggacgta
     7501 caggggctaa atcggctaaa ctacagtttt tctgattttt aatatgcgtt tagtgaagcc
     7561 agtaatgaag aaaccgctcc gccagcaaaa ccgccagatt attagctatg tcccacgcac
     7621 ggaacccgcg ccgccagaac atgcgataaa gatggattcg tttcgtgatg tctggatgct
     7681 gcgtggcaaa tatgttgcgt ttgtactgat gggagagtca tttctgcgct caccggcgtt
     7741 tactgtgcct gaatccgccc agcgttgggc aaatcagatt cgtcaggaaa atgaggtcga
     7801 agaataaaaa aacacctgtc gggatgacag gcgtttaacg taacaaatca ggattaacgg
     7861 tgtgccagtt cggcgtcgtc ttcgctatcc agaatagctt tatcggtttg cttcagccac
     7921 tggctggtca gcgtgccagc ggtcatcgag ccactaacgt ttagcgccgt acgacccatg
     7981 tcgataagcg gttcaacgga gattaacaat gccaccagcg ttaccggcag gcccatcgca
     8041 ggcagcacaa tcagtgcggc gaaagttgca ccaccaccga caccggcaac gcctgcggaa
     8101 ctaacggtaa caataccgac cagcgtcgca atccacatcg ggtccatcgg gttaatgcca
     8161 accgtaggcg caaccatcac cgccagcatc gccggataca aaccggcaca gccgttctga
     8221 ccaatggttg caccgaaaga ggcggcgaaa ctggcgatgg attcaggaac accgagtcga
     8281 cgcgtttgtg cttccacatt cagtgggata gacgcagcac tggaacggct ggtaaaggca
     8341 aacgtcagta ctggccatac cttacggaag tacttcagcg ggctcacgcc attaataccc
     8401 agcagaaggc catgcactgc aaacataatc agaagaccga ggtaggacgc gacaacgaaa
     8461 cttcccagtt tgatgatgtc ttgcaggtta gaacctgcaa ccactttggt catcagcgcc
     8521 agaacgccgt aaggggtcag ctgcatgacc aggcgaacca gtttcatcac ccagctttgc
     8581 agggtatcga tagcggttaa gacgcgttca cctttcggcg catcatcctt cagcagcttc
     8641 agcgcagcta cgccgaggaa tgcggcaaaa attaccacgc tgataattga cgtcggattg
     8701 gctccggtca aatcggcaaa cgggtttttc gggataaagg acaagaccag ctgcggaacg
     8761 ctcaggtcag agactttacc aacatagtta ctttcgatgg cgttcaaacg tgcagtttct
     8821 gcaccaccct gaaccagacc ttcagccgtc aaaccaaaca ggttggtgac cagcacaccg
     8881 accagcgccg caatcagtgt ggtaaacaaa agcgttccga tggtcagaaa actgattttg
     8941 cctaactgtg atgcgttatg cagacgggca accgcgctca gaatagaggc aaagactaac
     9001 ggcataacga tcatttgcag gagttgaaca tagccgttac caacaatgtt aaaccactgt
     9061 acagaatctt taagtacctg gctgtcagaa ccataaatgg tatgcagggc gaggccaaaa
     9121 accacaccca tcaccagacc caccagcact tttttcgcca gactccactg tttatggcgg
     9181 gtctgagcca gcgcaaacag cagtacaacg aacaccacga tgttcgcaat taatggaaag
     9241 ttcatccccg ttctcctgat cttattattt ctatcactgc gtagcagtca tagtgttggc
     9301 gcaagattaa cagaactgtg agctggctct tatattcaaa tggaattgct tattccagaa
     9361 aggtgatttt tgccttttta gtattcaacc tgatgcttaa taagccgatg aaactggatc
     9421 tgcaaggtat tgatcatttg cgatgaccaa cggacagcgc tgttttcggg taatgaatgg
     9481 ggcatccata ccgaccagac aaataatgcc atgcagataa aacgttccag ttggttgccc
     9541 ttttggggaa ccaggatagg caagcggaaa cgccagcggc atggccagag caggggaaca
     9601 ccggcgggtg tcagcatatc ggcaagtatg tggctcaaat aacccagcac cattccttgt
     9661 agcgcatcag ccggaatgaa ccagctttca ggaaccttaa ggtaaaaggt tgccagcagc
     9721 gcaaataccg ccagcagact gtgggtaaaa ccacgatgcc caaaagcgcg ggcgattggt
     9781 tttgatatcc actttaatcg ctgcccaaga aacgactttg ggtgatcgat gtccggtaac
     9841 aaacacgtca ggattgcgga agggacaata tgccaccagt caccctgtgc cagcacgggc
     9901 gtcagctcgg catttttggc aaataccgca caagcaatag aaaagagaag gtgaccttcc
     9961 gccgtcatga taaaactcac taaactgtcg atacgtacag tatagggttt ttatacagtg
    10021 cacggaagtg gtgacggtgt aactctttct cttaaattgg cgattaatcc gtcagttgaa
    10081 tgcaaattca gataaagaac caggggcagt gtgatcactg ccccctggtc attaaccgag
    10141 aaggtctttt gcggtgagtt ctgtcagcga tgagagtttg acgtttgcta atacaaaacg
    10201 tggatcattt tgcgcttctg gcgcaggaac gacgatggaa cgcatgcggg ctgctttaga
    10261 ggcgatcatg ccatttaccg aatcttccag tgccacgcag gtcagagggt caacccccag
    10321 ttttgctgcg cagtcgatat atacttgcgg atgcggcttg ctgtaaggca atttttcagc
    10381 cgaggcgagg gcatcgaaac tgtcgcgtaa atcaaacatg gtcaatactt tttccagcat
    10441 atgcagcggt gacgcggagg ccagccccac caataaacct tgttctttgc ataacgcaac
    10501 ggcttcacgc acacctggta ataatggacg cgtctcttca accagtgaaa tggcacgagc
    10561 aataacttgt tctactactt cctgacggct tggcccattc catggttgcc gggcgtacca
    10621 aagatcgacc accatatcga tgcgtaaacc taaggtgtcc ggcagctcgt tacgacggga
    10681 gatatccacc cccaggcttg ccatcacatc cagttcggct cgatcccata aaggttctga
    10741 gtcgataagt aatccatcca tatcaaaaat tgcagcaaga atctgacgcg gggttgacat
    10801 tacaacctct cctttagagc attatatgta aggtattaag ccgtgcttat gattaagcgc
    10861 ctatcatatc gcctttaaag aatgggcgaa aacagatatc tacaggtaaa ctaagtgaaa
    10921 cgacgcgtct taatcaagga gaactcatga cgtatcaaca agctggacgc attgctgttt
    10981 tgaaaaggat tttgggttgg gtgattttta tacctgcatt gatctctaca ttgatttctt
    11041 tgcttaaatt tatgaatacc cggcaggaaa accaggaagg cattaatgcg gtcatgctcg
    11101 actttactca tgtcatgatc gatatgatgc aggcgaatac gccttttttg aatctgttct
    11161 ggtataactc cccgacgccc aattttaatg gtggcgtgaa cgttatgttc tgggtgattt
    11221 tcttcctgat ttttgtcgga ctggcgttgc aggattccgg tgcccggatg agtcgccagg
    11281 cgcgcttctt gcgggaaggt gttgaagatc aactcattct ggaaaaagcc aaaggggaag
    11341 aggggctaac gcgtgaacaa attgagtcac gtattgttgt gccgcaccat actattttcc
    11401 tgcagttttt ctcgctgtat atcctgccgg ttatttgtat tgctgcaggt tatgtgttct
    11461 tttctctgct ggggtttatt taatcaaaag gccgcacgga gcggccatta tcctcacccc
    11521 atattatgct gctaataatc tatccagtga ctgctgagca ataaccaaat gctgaccgcc
    11581 aaataacctt gcacgattta acagcgtgta gagttggtaa accggttgac gttcaaggaa
    11641 atcggcaggt agcggggata ctgactgata gccgtcatag atttgtggcg gctgttcagt
    11701 atgcagcggt aacatcgcca ggtcgcactc tcggtcaccc cagtagcagg ccgggtcgaa
    11761 aatgtacggg ccatccggac ccagcgcaca gttgccggac cataaatcgc cgtgcaacag
    11821 agaaggctgc ggttgatgcg aggccagacg ctgctgaata tgctcgacga tggcatcgat
    11881 attgccgaaa gcgatccctt tttctgccgc cagttccagc tgccagccaa tccgttgttc
    11941 agcaaaaaaa gttgaccagc gacgttgcca ggtgttgggc tggggagttg tggagagcgc
    12001 gttatcgaaa tcgaggccaa attgcggttg gtcgctccat tgatgtaaac gcgcaatttg
    12061 ctgaccaaga ataaatgcgc tatgcgcatc cagcgggcga ggtgggagat aatccatcac
    12121 cagaaaactg tagtcacggt cagcgccaac tgcccaaacc ttaggcacgg tgacggtttt
    12181 actacgcgac agtaactcca gttggtcggc ttcggcggta aaaccgggaa gcagttccct
    12241 ttcatcacat ttgacgaaaa agtcatgtcc tgcatagcgc aaatgccatg cggcatggac
    12301 ttctccgcca ggcagttcat tacgcagttc gatttcgcct tcacctaact gctcgctcaa
    12361 aagacgactg attgcctgcc acatgatgtc tctcccatgt tgtctgccag atcataaggt
    12421 tagcgcaaga atgcggtgta aaaacacgag ctaacgcaca ccacagactg attattgccc
    12481 aaaaaccatt tattttctgt tgctattcca ttcctcccag gtcgtgatgg ctatttcagg
    12541 atctttaccg gttgcacttt gggtaagccc cgaaacaagt tctctaattt cttcttcact
    12601 ttgggtactg ataagtccaa aagtattagt ccccagctca tggatatttc catcatcatc
    12661 cgtcatggtg agcaaaaaac cgtcgcgagt caggtaatta ttcaactcgt taatttcagt
    12721 caatgtatct tcgtgcaaca tgacggttat gacataacgg acaagatcgc cactggccat
    12781 agttcacccc tttgttatca catgactttt gagcatagtc ggagaaacgc gttgccgaca
    12841 ggttggtggt gattccccca atgctggggg aatgtttcag ttagcgggaa aggtaagcgt
    12901 aaattttttg tgtatcgtca tgggagcaca ggcgtgttcc ctgattgagt gtggctgcac
    12961 tccccgcagc tacgccaaaa cggaccatct cttcaagaga ggcattttct gccagtttca
    13021 gtgtcatcgc gccgaccatg ctgtcaccag cgccaacggt gctctggctt ttcaccggtg
    13081 gtggcacaac ctgaatgcag ttttcactat caacgcccag cgccccttgt ggacccaggg
    13141 aaacgacaac tcgtttggcc ttgccgctat taacgatttc ctgcgcggct ttgcggacat
    13201 cgtctggctg ggtgagttcg cgattcacca gcgcactgag ttctttttgg ttaggcttaa
    13261 ccaactcgat gttaccaatc gccagtgctg cacttaacgc ttcgccagaa ctgtcgacga
    13321 tacagcggat cccttggttt tgcgcagcgg aaatcagttg ggttaatttt tccagcttca
    13381 cacctagcgg caggcttccg cttatgacca ggatagcacc ggattcaatt tccagaactt
    13441 gctcttcaag ctggcgaaac tcatcttcat ttaatgccgc gcctggcatg acaaaacgat
    13501 actgctcacc gcttgcttcc acatgtacgt gtaaattctg ccgggtccag tctttggctt
    13561 ctacagtagc gacggggaca ttttcatctg ccaacagtga aaccagatgt tcgccggtcg
    13621 cacctcccac cgggaagatc gctgtagcac tgcctccaag atgggcaatg gcgcgggcga
    13681 cgttgatgcc gccgcctccg ggttcgaata ccggtgcggt acagcgcagc tttccttcgg
    13741 gataaatttg cggggtaatt gttgcgctat cgagagaggg cgcaagtgtc aacgtataga
    13801 tacgtaccat catttcctcc tataggctga tttcagtctg gcaccgaatc agcgga
//
LOCUS       DAZKGF010000131        13735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZKGF010000131 DAZKGF010000000
VERSION     DAZKGF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.131"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..1117
                     /gene="tssD"
                     /locus_tag="ACHOQV_003684"
     CDS             2..1117
                     /gene="tssD"
                     /locus_tag="ACHOQV_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFP9631982.1"
                     /translation="MTITGEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSLSNSITN
                     TGKGSQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYYNIQLRG
                     ALLSAINHLFTENNLDTETITVSYEYILSRHLIANTEFSHLAFPDNYNRLFIPRQKTK
                     DDNGLKTLNSKAVGRLLAAGGIYNGNIEGFRETANKLGGDAPAGYDQVMDNKGLLIAG
                     ASIVAGVVLGRMRFPDLESFEHFGARGAVSGRPFKPDLAGGPIENLTIDGVTINREGI
                     AIVEKHISRFDHDPANDVMISRLKKIANKELLPEKYDLNYYTHECREYQRYCNLGWET
                     GEPNGLDGYELWNNAHTATLEDFKIKDTDLFHPDAKK"
     gene            1114..1371
                     /locus_tag="ACHOQV_003685"
     CDS             1114..1371
                     /locus_tag="ACHOQV_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9631983.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSKPRYRVSQYGLNLIKEQQDNLF"
     gene            1446..1691
                     /locus_tag="ACHOQV_003686"
     CDS             1446..1691
                     /locus_tag="ACHOQV_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9631984.1"
                     /translation="MITKNELNVLNVMTEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVSEKGYNLVQRENK"
     gene            complement(1860..4121)
                     /gene="metE"
                     /locus_tag="ACHOQV_003687"
     CDS             complement(1860..4121)
                     /gene="metE"
                     /locus_tag="ACHOQV_003687"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP9631985.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            4239..5192
                     /gene="metR"
                     /locus_tag="ACHOQV_003688"
     CDS             4239..5192
                     /gene="metR"
                     /locus_tag="ACHOQV_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFP9631986.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(5080..5979)
                     /gene="bioP"
                     /locus_tag="ACHOQV_003689"
     CDS             complement(5080..5979)
                     /gene="bioP"
                     /locus_tag="ACHOQV_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFP9631987.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(6055..6855)
                     /gene="yigL"
                     /locus_tag="ACHOQV_003690"
     CDS             complement(6055..6855)
                     /gene="yigL"
                     /locus_tag="ACHOQV_003690"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFP9631988.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(6863..7885)
                     /gene="pldB"
                     /locus_tag="ACHOQV_003691"
     CDS             complement(6863..7885)
                     /gene="pldB"
                     /locus_tag="ACHOQV_003691"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFP9631989.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVIVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7996..8616
                     /gene="rhtB"
                     /locus_tag="ACHOQV_003692"
     CDS             7996..8616
                     /gene="rhtB"
                     /locus_tag="ACHOQV_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFP9631990.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKPL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(8678..9298)
                     /gene="rhtC"
                     /locus_tag="ACHOQV_003693"
     CDS             complement(8678..9298)
                     /gene="rhtC"
                     /locus_tag="ACHOQV_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFP9631991.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRSALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIIET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(9362..11191)
                     /gene="recQ"
                     /locus_tag="ACHOQV_003694"
     CDS             complement(9362..11191)
                     /gene="recQ"
                     /locus_tag="ACHOQV_003694"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP9631992.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENDVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPISASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(11323..12192)
                     /gene="pldA"
                     /locus_tag="ACHOQV_003695"
     CDS             complement(11323..12192)
                     /gene="pldA"
                     /locus_tag="ACHOQV_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFP9631993.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITRHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            12357..12824
                     /gene="yigI"
                     /locus_tag="ACHOQV_003696"
     CDS             12357..12824
                     /gene="yigI"
                     /locus_tag="ACHOQV_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFP9631994.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            12876..>13735
                     /gene="rarD"
                     /locus_tag="ACHOQV_003697"
     CDS             12876..>13735
                     /gene="rarD"
                     /locus_tag="ACHOQV_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFP9631995.1"
                     /translation="MDTKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPKKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDA"
BASE COUNT         3431 a         3584 c         3420 g         3300 t
ORIGIN      
        1 cctgaccata accggagaac aacaaggatg tatttcctct cgttgcggaa cgagcgcatc
       61 aataggtaat cgctggcaga ttggccatga agatgaaatc ttcgcgttct cgctatctaa
      121 tagtattacg aatacaggca aagggtcaca attacatggc cttagtttct gtaagttgat
      181 tgacaaaagc tccccgttac ttattaacgc tattaacaat aacgagcagt tgttcatgga
      241 gtttgacttc tatcggatca atagatttgg tcgatgggag aaatattaca acattcaact
      301 ccgcggcgcg ctcttatcag caatcaatca cctgtttact gagaacaatc tggatacaga
      361 aaccatcact gtcagttatg aatatatact ctccaggcat ctgattgcta atactgaatt
      421 tagtcatttg gcatttcccg ataactacaa ccgtctgttt ataccacggc aaaaaactaa
      481 agatgataat ggcctcaaaa cactcaatag taaagcggtt ggcagactgc ttgctgctgg
      541 cggcatatac aatgggaata tcgaaggatt cagggaaaca gcaaacaagt tgggaggaga
      601 tgcacctgct ggttacgatc aagttatgga taacaaaggg ttactcatcg ctggtgcttc
      661 aatagttgct ggagttgttc tgggaagaat gagattccct gatttggaat catttgagca
      721 tttcggagcc agaggagcag tatcaggaag accatttaag cccgaccttg ccggtggtcc
      781 aatagaaaac ctgacgatag atggcgtaac aatcaatagg gaaggtatcg ctattgttga
      841 aaagcatatc tccagatttg atcatgatcc agctaatgat gtaatgattt caaggctaaa
      901 aaaaatagca aataaagaat tgcttcctga aaaatacgat ctgaactact atactcatga
      961 atgccgcgag tatcagcgtt attgtaattt aggatgggaa acaggtgaac cgaatggttt
     1021 agatggatat gaattatgga ataatgcaca tactgcaact ctagaagact ttaaaattaa
     1081 agatactgac ttatttcatc cggatgcaaa aaaatgacga ttacgatagt tgaatttaac
     1141 gtgctgaaag ttatggctga gaaagatatt gactggagct ggatggtgct ggatcgtact
     1201 ttagctatac gaaatattcc aggatttggc aacgtagcga atatcgtaac aaagctggtt
     1261 aatcatgggt tggttgatat cgtaaatggt gagggcaact ctaaaccacg ctacagagtt
     1321 tctcaatatg gattgaatct tatcaaagaa caacaagaca acctcttctg atccagccat
     1381 tacaggagca tgatttacat tgtgatcttt caataagata ctcatcaatt ttggtacaga
     1441 atatcgtgat cactaaaaat gaacttaacg tgctgaacgt tatgactgag aaagatataa
     1501 actggaactg gatgaatctc gatcgcacac tatccataag gcaaatacca ggattcggta
     1561 atgttgttaa tattgttaac aaattagcta acgaaggttt ggttattatt gaggatagcg
     1621 ggcataagtc tatgcctcac taccacgtta gtgaaaaggg atacaatctc gtgcaacgtg
     1681 agaataaata acgtagcgtt gattactgaa cggaatcact caaaactgtg ttttgcgaaa
     1741 aaaacgattc ggttatttct actcctgaag atctgcttca atctggcact catgcaaaat
     1801 ggcttccagt actggaagcc attatcgtac tgcaatttac aatgactcgt taatactcat
     1861 catccccgac gcaaattctg cgccgcctgc accatgttcg ccagtgccgc gcgggtttct
     1921 ggccagccgc gcgttttcag gccacagtcc gggtttaccc acagacgctc tgccggaatg
     1981 cgttttgccg ctttcttcag cagggcttca atccattcca cgctcggtac gtttggcgag
     2041 tgaatgtcat agacgcctgg accaatttca tttggataat caaactcttc aaacgactcc
     2101 agcaactcca tgtcggaacg cgaggtttcg atggtgatga cgtctgcgtc cagcgccgca
     2161 atcgaatcca tgatgtcgtt gaactcgcag taacacatgt gagtgtggat ttgtgtgtca
     2221 tctttcgcca cggcggcgtt gatacggaaa gcctccacgc cccactggag ataggcatcc
     2281 cagtcgctgc gacgcagcgg taaaccttcg cgcagtgccg gttcatcaat ctggatgatg
     2341 ccaattccag cggcttccag gtccgccact tcatcacgca gcgccagcgc aatttgtttg
     2401 gcgatggttt cacggctgac atcttcacgc gggaatgacc agcagagtat ggttaccggc
     2461 cccgtcagca tccctttcac cggtttgtcg gtcagcgatt gtgcatattt cgcccactcc
     2521 acggtaattg gtgccgggcg gctaacgtca ccaatgacaa tcggtggctt cacgcagcgg
     2581 gaaccgtagc tctgtaccca accgttttgc gtaaagacaa agccatccag atgctcgcca
     2641 aagtattcca ccatgtcatt acgctcggcc tcgccatgta ccagcacatc cagtcccaaa
     2701 cgttcctgct cgacaatggc ctgcttgata tgttccgcaa tgcccgtgcg gtagttgttg
     2761 gcgtcgagat tacccttttt gaaatccaga cgcagtgtac gaatttccgt ggtttgcggg
     2821 aaggaaccaa tcgtggtagt cggccacgcg ggcagtttaa aacgcgcacg ctgggcttca
     2881 gcacgcactt catagacatt cgcacgctga ctgtcctggg cggtaatggc tgcaagacgc
     2941 ttttctaccg ccggattatg gacacgggta gagtgacgac gcgcctgaat cggagcgctc
     3001 cattctgcca gagccgccgt atcaccactg ttcagcgcat cgcgcagcag tgccagttca
     3061 tggcattttt gtagggcgaa ggcaaaccag cttttcactt ctgcatcaag acgcgtttcc
     3121 acgctcaggt cgatggggct gtgcagcaac gagcaggaag atgccaccca caaatcgcgt
     3181 ttgccgacaa tgtccttaat ttgcgcatat ttctcggtaa gatcggcgcg ccagacgtta
     3241 cgaccattga tcagacccgc agacagtagc cagtcagaag gcagacgctt gtgcagttca
     3301 gcaacgtcat ctttaccatg tacaaggtcc acatgcagcc cctgaacagg cagcgcagta
     3361 atcgtgtcga gatttggcgt tacgccttca aaataggtag tcagcagcag tttcacctgg
     3421 ccctggagcg cgtcgtaagc gggtttgtat gcgtccagcc acgcctgcgg tagttccagt
     3481 accagcgctg gttcgtcgat ctgtacccac tcaatgccgc gttttgccag ttctgccaac
     3541 acttgctgat aaaccggcag aatgtcgttc agcaggctca ggcgatcaaa ctgttcacct
     3601 ttcactttac ccagccacag gtaggtaacc ggccccagca gcacgggctt gaccttgtgg
     3661 cccagcgcca gcgcctcgtc cacttcctcc agcagctgcg tccaggtcag tttgaactgt
     3721 tggcctttaa cgaactccgg caccatgtag tgatagttgg tgttaaacca tttggtcatt
     3781 tccgctgccg ccgcaggttc gccagtcggt gcacgtccac gaccaatacg gaacagggtg
     3841 tcgatatcta ccgaaccatc tttgttctga tgacgtggcg gaacgttacc cagcagcaga
     3901 ctggtggtca gtacatgatc gtaccaggca aaatcgccca ccggcagcag gtcgataccc
     3961 gcctgctttt gttgatccca gtgacgggca cgtaattcac gcccaacagc cagcagttct
     4021 tcacgcgtgg agttccccgc ccaataactc tcttgcgctt ttttcagctc gcgacgcagg
     4081 ccaacgcgag ggaaaccgag ggtgtgatta agaattgtca ttatatgccc cttatggaat
     4141 ttttagtatt tagccgtcca gatgtttaca catccataat tggcggttac tgtatattcc
     4201 tcaagcgcaa attattcatg ccgaagtgaa ggactttcat gatcgaagta aaacacctga
     4261 aaacgctaca agcgttgcgg aactgcggct cactcgcagc cgctgcggcg acgttgcatc
     4321 aaacgcaatc cgccctgtct caccagttta gcgatctgga acaacgcctt ggcttccggc
     4381 tatttgtgcg taaaagccag ccgcttcggt ttacaccgca gggagaaatc ctgttgcaac
     4441 tggcgaatca ggtgctgcct caaatcagcc aggcactgca agcctgcaat gaaccgcagc
     4501 agacgcgcct gcgcattgcc attgagtgcc atagctgtat tcagtggctg acgcccgcgt
     4561 tagaaaattt ccataagaac tggccgcagg tggagatgga ttttaaatcg ggcgtgacgt
     4621 ttgacccgca gcccgcgttg cagcagggtg agctggatct ggtgatgacc tccgatattc
     4681 tgccgcgcag tggcctgcat tattcgccga tgttcgatta tgaagtgcgt ctggtgttgg
     4741 cacctgacca tccactggcg gcgaaaacgc gaatcacgcc ggaagacctc gccagcgaaa
     4801 cgctgttgat ttatccggtg cagcgtagcc gactggatgt ctggcggcat tttcttcagc
     4861 ccgcaggcgt tagcccgtca ctgaaaagcg tcgataacac cttgttgttg attcagatgg
     4921 ttgccgcgcg gatgggcatc gctgcgctac cgcattgggt ggtggagagt tttgaacgcc
     4981 agggtctggt ggtcaccaaa accctgggcg aaggcttgtg gagccgactg tacgccgccg
     5041 tgcgcgatgg cgagcagcgt cagccgatta cagaagcgtt tattcgttca gcgcgcaatc
     5101 acgcctgcga tcatctgccg tttgtgaaga gcgcggagcg acccacttac gatgcaccca
     5161 cagtgaggcc aggatcacca gcgcgcctgt aataaacgtt ggccagtgcg gctgttggtg
     5221 ccagatagcc aggttaacca acagccctgc cggaacgtgc atattattca taatgcccag
     5281 cgttccggcg tccacctgag tcgcgccgta gttccacata aagtagccaa taccggaagc
     5341 caccacgcca agaaacacca gaatgcccca ttgcagcgtg gtttgcggca ttttctgcgc
     5401 atttcccagc aaaaaccatg caatcaccgc caccaggaac gcaccaagat agaaccacgc
     5461 aaaggcgtta tgctgtggca tcggacgagt ttccatcagg cgtttgtaac ccaccatgcc
     5521 gatggcaaaa gtgatattgg agagttgcac cagcagcagg ccagtccaga agtggtcggt
     5581 gacctgatca tagcgaataa ttccggcacc aatcaccgcc aacaaggcgc taaaagcata
     5641 gccccagcgc agacggcgct tactcatgat gtcataaatc agcgtgatgt agagcggagt
     5701 cagtacggtg aacagcaaca gttcggagac cgtcaggtag agataggcgc gaaaactcag
     5761 catatacatc acaccgagct gcatcgcgcc caccagcata tacaggccaa ccgttttcag
     5821 gctattgcca cgggtacgca gaaacggcag aaacacgagt gccgccaggc caacgcgcac
     5881 cagcactgca aaatagctat cgacgtgccc cgcaaggtac tcgccataaa agctaaagga
     5941 gaaggcccac agaatcgtgg tgatgataag tagcgccaca atggaaaact ctttgattaa
     6001 cgagtatcca ttgtagcgaa gtatcaggtt gacaactgac caaataaaga acgattacga
     6061 taaatagagt ttacgcagat aatgcggcac cgcgtcttcg gcattagtac caatcacttc
     6121 cagctccgga tgcagatctt tcagacgctg gtgcgcactg cccataatgc agcctttacc
     6181 tgccatcgac agcatttcgg cgtcgttcat cccgtcacca aaggcaatac aatctttcag
     6241 actgtagccc agtttcttcg ccaccgcttc cagggcatgg ccttttgaaa cgccgcccgc
     6301 catcacttcc agacaagtta gggtagagaa gctgacgttg acgcgatcgc cccaacgagc
     6361 gttgattgcc tgctccagcg gcagcagttg ctcatgagaa tcgcaggtga agaacacttt
     6421 gctgacgcct tccggctcca gtaatcctgg ctcatacagc gcatatttga acactgcttc
     6481 tttaaaaaag cgcatctctt ccgggcggtg gcgattcata aaccattcgt cgtcgcgata
     6541 aacgttagta atgatgtccg ggttgtcgtt gaccacgcca aacagatcgc tggcaatgtc
     6601 gcgatccagg ttatgagcaa aaatcagatt gccatccaga tcgtgaacac gcgcgccatt
     6661 ggaggtaatc atgtaagact taatctccag attatcgcga atttgcccta catcaacgtg
     6721 gtggcgaccg gtcgcaaaca caaagttaat gccgcgcgcg gtgagcagct tcagagtttc
     6781 tttggcgtaa ggggataacg tatggtcggg agaaagtaac gtgccatcta aatcagacgc
     6841 aacaacctgg tacataagaa atttaaacct ctgtagagcg gtttccgctg ggtgagttat
     6901 gcctgttgaa aaaatcaaca atggcgtgga gcgcgactga gcgcattgcg tccttttcaa
     6961 aaaggatctc atggtacgca cctttaatta ccagcggtcg tcccccttcg acaggatggc
     7021 ccgcagcggt gcggagttca caaaaacggt cgtgcatgcg gttatccacc acgcgttcct
     7081 cttcagcctg caagagaagc gttggcgtgg cgtcatcacc cgcaccggct aacacctgtt
     7141 cgccagccag aatactttcg cgtacccaat ggtaggtcgg cccaccgacg cgaatcgttg
     7201 ggtcatcagc ataaaagcgt aagttacgtc gatatcgctg tctgctgtgg gtcagtacgt
     7261 tgatagcaaa cggcaacgcg cgccagcgcc cggtgcctat tgcatagcca tcacggaaac
     7321 gtggatgcgc ttcggcccag ttaaggatct gccgcgccat aaatgacggc atacgaatca
     7381 cgatcccaaa cattggcgca gttagcgcaa tggcgtcaca tacacctgga tggcgttgca
     7441 gaaataacgt ggagatcgca ccgcccatcg aatgtgccag tatatagcgc ttacgccacg
     7501 gaccaggctg gacctcctgt tgccagaatg ccgccaggtc atcgacataa tcattaaagc
     7561 gattaacatg cccaagatgc ggatcggcta acaggcgacc ggaacgtccc tgcccgcgat
     7621 ggtcgatgat taagacatca aaccccaaat ggaacaggtc gtaggccagt tccgcatatt
     7681 ttacgtagct ctcaatacgc cccgggcaga tgactatcac ccggtcatgg tgctgtgcgc
     7741 gaaaacggac aaagcgcacc ggaatgtcat ctacaccagt aaactctgct tcatcacgct
     7801 gacgccagaa atcagtcagc ggtcccatgg taaaagcagc aaacgcgttt tctcttgttt
     7861 cccagtcttt ttgctgctga aacatcgggt aatctgcctc ttaaaccacg taaaatcgtt
     7921 tttttaagcg tgcctgacac aacgcttcga cagtagcgta ttgtggcaca aaaatagaca
     7981 caccgggagt tcatcatgac cttagaatgg tggtttgcct acctgctgac atcgatcatt
     8041 ttaagcctgt cgccaggctc tggtgcaatc aacaccatga ccacctcgct caaccacggt
     8101 tatcgcggcg cggtggcgtc tattgctggg cttcagaccg gactggcgat tcatattgtg
     8161 ctggttggcg tggggttggg gacgctattt tcccgctcag tgattgcgtt tgaagtgttg
     8221 aagtgggcag gcgcagctta cttgatttgg cttgggatcc agcagtggcg cgccgctggc
     8281 gcaattgatc ttaaaccgct ggcctctact caatcgcgtc gacatttgtt ccagcgcgca
     8341 gtttttgtca atctcaccaa tcccaaaagt attgtgtttc tggcggcgct atttccgcaa
     8401 ttcatcatgc cgcagcagcc acaactgatg cagtatatcg tgctcggcgt caccactatt
     8461 gtggtcgata ttattgtgat gatcggttac gccacacttg ctcaacggat tgcgttatgg
     8521 ataaaagggc cgaaacagat gaaggcattg aataagattt ttggctcgct gtttatgctg
     8581 gtaggagcgt tgttggcatc ggcgaggcat gcgtgaaaaa taatgtcgga tgcggcataa
     8641 acgccttatc cgacttactc tgatgaagcg tttggcatca acgagaaata atcaaatgaa
     8701 tgccaaatcc ggcaaataac gccccggcaa aaccatcaat ccacttcgcc agacgttgat
     8761 aaccacggcg catttgcggt agagcaaaca ggctggcaac caccgtaaac catgccagtg
     8821 tttcgataat aatcaacgcg aaaatgcccc agcgctcagt ggtgccgacg ttatcgccga
     8881 caaacagcga gaataccgaa ccaaagtaga taattgcttt cggattggcg agattggtca
     8941 gtaagccttt caggaaactg cgcccgcttt ttgccagttc tacctgtggc gcaggcgcag
     9001 aaacgacctc ttttttcagt gcgctacgca gcatctgata acccatccag cagagataca
     9061 ggccaccgcc caccataatc agcgtatgca gccaggccat tttttcgata atcaaatgca
     9121 ggccaagcaa cgcgatccct gcccagacca ttacgccgca ggtaatgccc agcacgccca
     9181 tcatcgcctc tttacgggaa cgactgacag cggtctgaga aacaaagaag aaatcgggac
     9241 cggggctcat aagcgccaca atgtgcacca tggcaacggt gaggaataac atcaacattc
     9301 aattgactcg cggagaaatc gtttacggag tcttcatcct ggcacttttt tatgctgctg
     9361 actactcttc gtcatcgcca tcgacatgcg cacgaattag cgccataaac ggtttgccaa
     9421 agcgttccag cttgcgcatc ccaacgccgt taacgctgag catttcgctg gcggagatcg
     9481 gcatctgttc cgccatctca atcaaggtcg catcgttaaa caccacatac ggcgggacat
     9541 tactttcatc ggcaatcgat ttacgcagtt tgcgtaattt ggcgaacagt ttgcgatcgt
     9601 aattgccacc gaaagatttc tgcattgctt tcggtttgag cgccacaata cgcggcacgg
     9661 caagttgtaa agaggattcg ccgcgcaaca ccgggcgtgc ggcttctgtc agttgtaatg
     9721 ccgaatgctg ggcgatgttt tgcgtcacca gaccgagatg aatcaactgg cggattacgc
     9781 tcacccagtg ctcatgactt ttatcgcggc ccatgccgta gactttcagt ttgtcatgac
     9841 catagtcgcg gattcgctgg ttattggcgc cgcgaatcac ttccactaca tagcccatcc
     9901 caaaacgctg attcacacga ccaatggtgg aaagcgcaat ctgcgcatcg gttgaaccat
     9961 cgtattgttt cggcggatcg aggcagatat cgcagttccc gcacggctcc tgccgccctt
    10021 cgccaaagta gttcagcagc accagacgac ggcaagtttg cgcttcggca aaagcgccca
    10081 tcgcattgag tttatggcgc tcgatatcct gcaactgccc ctgcggcttc tcttccagac
    10141 aacggcgcag ccacgccata tcagccggat cgtaaaacag catcgcttcc gcgggcaggc
    10201 catcacgccc ggcgcgaccg gtttcctgat aataggattc gatattgcgt ggaatatcaa
    10261 agtgaaccac gaagcggacg ttaggcttgt tgatgcccat gccgaacgct accgtcgcca
    10321 ccacaatttg caggtcatca cgctggaatt tttcctgcac gtccgcacgg acatcgtttt
    10381 caagcccggc gtgataagcc gctgcgctga tacccttgct ttgcagacgc gcggcggtgt
    10441 cttctacttt cgcacgacta ttacagtaaa taatccccga cttaccgcgt tgttcctgta
    10501 cataacgcat caactgatcg agcggtttga atttctccat cagcatgtag cgaatattcg
    10561 gacggtcaaa actgctaatt tgaatcagcg gatcgttcag ccccagcagg cgcacgatat
    10621 cctgccgcgt ggtgtcgtcg gcggtggcgg ttagcgccat aaacggcagc gtcgggaacc
    10681 gctggcgcaa ctgaccgagc gcggcatatt ccgggcggaa atcgtggccc cattgggaga
    10741 tacagtgcgc ttcatcaacg gctaataaca ccggattcca gtgcgccaga tgctcaagaa
    10801 agttatccag catcaggcgt tccggggcga tataaagcag acgaatttgc ccggtgcgac
    10861 agcctgtcat cacttcaagc tgctgctcgc gggtttgcgt cgagttaagg cacgccgccg
    10921 ccacaccgtt ggcctgtagc tgatccacct gatctttcat caacgaaatc agtggtgaaa
    10981 caaccacggt aagaccgttt agcagtaagg caggaatttg atagcaaagg gattttccgc
    11041 caccagtggg catgacgacc aggcaatcgc gaccggaaag tacggtgtcg ataatctctt
    11101 cctggccggg gcgaaactgt tggtagccaa aggtttcttg taaaacctgt ttagctccgg
    11161 actccagatt caacacttcc gcctgcgcca cattaacccc gattgcctga aataaaaaca
    11221 ggcgctattt tcagcgcctg cgcgagaaac tgcaatgctt ttagtctctg agagggtaat
    11281 cctggatact ggattccagt tttgcctttc aatcaactca cattagaaca agtcgttcag
    11341 cataaccccc acacccacac gggtctggtt aaagttatag tcgatgagcg attcgccata
    11401 accgctgtaa acctgggtat aaaggcgcac atgcctggtg atcgggtaac ttaagcctaa
    11461 ctccgcacca ccgtaaccgg tgttccagtt gtactgtcct ttcgcactca gcaccgcatc
    11521 acccaggtga tagccgattt taagctggta gtaacccata tatttggtga tatctgggtt
    11581 atcgtcagta ttccccacca cataccacgg cttcacctct accagccagt taccgttttc
    11641 tgccatcaga cgggtataaa ggcggttcca gctgcgggaa gtcgggtctg aacgcccgtt
    11701 agagtcgtgg ttataaccca tctccacatc gcgcagcgtc cagccagcaa agcggtaatc
    11761 ggtggcaaaa ccaaggaaca attgcggttc gtagttggtt tcacgaaacg gtgaagactc
    11821 ttcgctattg gacagttgcc accaggattt ttgcgtgtaa gaagccccca acaccgagtt
    11881 tggccccaat attccgcgcc acagcggaaa tgccaggctc aactggaatt tcacttcatc
    11941 cttacgcgcg ttttccgccc agtcataact ggcgatcgct tctttgttca gatcgctggt
    12001 ttgggtgtag atgaggtagt tggtgtcata aggatagagc gtgaaaggat tgtcatgctc
    12061 ctgcagcata ttcgcaataa tactgccacg caccgcaggc gcgtcatgca cctcttttac
    12121 cgttgcctct tgcgcataca ctgccattgg caacataaac accggcaaca accagccctg
    12181 cagagtccgc atcggtcgtg ttctccgtaa cgaattattg attagatgaa tatttttctg
    12241 cgggctattt tacatactta actcctttgc cgttagttat cccttcctaa agtagaaaac
    12301 cacaattaag aagcataaaa tcaacatttc attaaccaat gaggtgtaag gatgatatgt
    12361 ctgccgtact gaccgctgaa caagccctga aattagtggg tgagatgttt gtttatcaca
    12421 tgccatttaa ccgcgcattg gggatggaac tggagcgtta cgaaaaagag ttcgcacagc
    12481 tggcctttaa aaatcagcca atgatggtgg gtaactgggc gcaaagcatt ttgcacggcg
    12541 gggtcattgc gtcggcgctg gatgtcgccg caggtctggt gtgcgtggga agtaccttaa
    12601 cccgccacga aaccatcagt gaagatgaac tacgccagcg gctatcgcgg atggggacca
    12661 ttgatcttcg cgtcgattat ctgcgcccag gcaggggcga gcgttttact gctaccagta
    12721 gtctgttgcg tgcaggcaat aaagtcgccg ttgcccgcgt tgaattacac aatgaagaac
    12781 agctttatat agccagtgcc accgccacct atatggtagg ttgataacgt aaaagcaggt
    12841 aaactgcttt taccaataaa aaatgagttt tgccaatgga caccaaacaa acgcggcagg
    12901 gcgtattact cgctcttgcc gcttatttta tttggggtat tgcgccagcg tacttcaagc
    12961 tgatttacta cgtgcccgcc gatgagatcc tgacgcatcg cgtgatctgg tcgtttttct
    13021 ttatggtggt gctgatgagc atttgccgcc agtggtccta tttaaaaacg ctgattcaga
    13081 caccaaagaa aatcttcatg ctggcagtct ctgccgtgct gattggtggc aactggctac
    13141 tgtttatctg ggcggtgaac aatcaccata tgctggaagc gagccttggt tactttatta
    13201 acccgctggt gaacattgtg ctggggatga ttttcctcgg cgagcgattc cgccggatgc
    13261 aatggctggc ggtgattctg gcgatatgtg gtgtattggt ccagctctgg acttttggtt
    13321 cgctgccaat catcgcgctg gggctggcgt ttagttttgc cttctacggc ctggtacgca
    13381 agaaaattgc cgttgaagcg caaaccggca tgttaatcga aaccatgtgg ctgctgcccg
    13441 tggcggcgat ttacctgttt gctattgccg acagctcaac cagccatatg gggcaaaacc
    13501 cgatgtcgct gaatttactg ctgatcgccg ccggtattgt caccaccgtg ccactgttat
    13561 gttttaccgc agcagctaca cgcttacgac tctcaacatt aggctttttc cagtacattg
    13621 ggccgacgct aatgttcctg ttggcagtga cgttttatgg tgaaaaaccg ggtgccgata
    13681 agatggtgac tttcaccttt atttgggtgg cgctggcaat ttttgtgatg gatgc
//
LOCUS       DAZKGF010000132        13681 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZKGF010000132 DAZKGF010000000
VERSION     DAZKGF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.132"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..303
                     /locus_tag="ACHOQV_003698"
     CDS             <1..303
                     /locus_tag="ACHOQV_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFP9631996.1"
                     /translation="LLLLPVILLPHTAMANTLFDMIKTVGDGAKGSSSSFVAIAQVIG
                     LVLFIAGLLGLKQIGKKPGAGLACILSIVVGACLAVVPEMMSSGQKTLGLSPVSIS"
     gene            360..611
                     /locus_tag="ACHOQV_003699"
     CDS             360..611
                     /locus_tag="ACHOQV_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFP9631997.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            526..1344
                     /locus_tag="ACHOQV_003700"
     CDS             526..1344
                     /locus_tag="ACHOQV_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFP9631998.1"
                     /translation="MNKTPPLIAPARERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRIRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILMPDSFPDKKYVM"
     gene            1399..1758
                     /locus_tag="ACHOQV_003701"
     CDS             1399..1758
                     /locus_tag="ACHOQV_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9631999.1"
                     /translation="MDTLTAGYQFSADAFRRFIIRTLTDDIPPDGDEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHNSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            1758..2138
                     /locus_tag="ACHOQV_003702"
     CDS             1758..2138
                     /locus_tag="ACHOQV_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632000.1"
                     /translation="MKIHRISPETLITLILAHLAGKADSTAKEEHRLLRRFLRDDDGR
                     LAGILLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     RNERRKCIVVHINKITYPPVNSLR"
     gene            2314..5358
                     /locus_tag="ACHOQV_003703"
     CDS             2314..5358
                     /locus_tag="ACHOQV_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9632001.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNIGHKISFVFERDPERGRNEIEVMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGSGPVPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISNLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHMAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQELGSLRARQI
                     LAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            5358..5975
                     /locus_tag="ACHOQV_003704"
     CDS             5358..5975
                     /locus_tag="ACHOQV_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFP9632002.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMSDNTWLWQDSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHS"
     gene            5936..7141
                     /gene="traW"
                     /locus_tag="ACHOQV_003705"
     CDS             5936..7141
                     /gene="traW"
                     /locus_tag="ACHOQV_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFP9632003.1"
                     /translation="MQRQPATTLLALLIGCAVIPSTHAYSVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPIREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            7138..7707
                     /gene="traX"
                     /locus_tag="ACHOQV_003706"
     CDS             7138..7707
                     /gene="traX"
                     /locus_tag="ACHOQV_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFP9632004.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            7780..9939
                     /locus_tag="ACHOQV_003707"
     CDS             7780..9939
                     /locus_tag="ACHOQV_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFP9632005.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNNQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     YYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTTASSTEYRTAEAAEPKSIIMKVWSGVNFADKIATSLSGTGTTSD
                     QLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSIFGRLVNAVSGLSDAAKAF
                     LEAAAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWAAT
                     HLGTNQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFGAALVNV
                     QADSLTGLFSIAGFLMIYARACTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNMVS
                     SVKDIIAGGNTNIRRTPGVREEKLKNINGDDKDGIKQ"
     gene            10021..10668
                     /gene="excA"
                     /locus_tag="ACHOQV_003708"
     CDS             10021..10668
                     /gene="excA"
                     /locus_tag="ACHOQV_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFP9632006.1"
                     /translation="MNTTKSRYPSWWAYYQLARNTYFIFGIPCLLFYAFFGTLIVHDS
                     SLSQTRDFIFAYGCWALIILPLLWLYLKTRAKKKKVQQVVQQIKESSPFAPTNDYEQL
                     SFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSLP
                     MIPLEISGISARTLANTIHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            complement(10982..11134)
                     /locus_tag="ACHOQV_003709"
     CDS             complement(10982..11134)
                     /locus_tag="ACHOQV_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9632007.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            11467..11709
                     /locus_tag="ACHOQV_003710"
     CDS             11467..11709
                     /locus_tag="ACHOQV_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632008.1"
                     /translation="MSNEYQSTSINPDVFEQFLTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            complement(11802..13337)
                     /locus_tag="ACHOQV_003711"
     CDS             complement(11802..13337)
                     /locus_tag="ACHOQV_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9632009.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNALGNVLILFLMMLLGRRNVISATCVCIFLLLSAFYAPVGMIYGKINNSFIASVMQ
                     TNINEAGEFITGIPVLYFIVSLVIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKNNQKDSWDIVSVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGQHDTLISVIAANADKKHFLKEGSSSSLRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHTNPCDRLFNDAQPFRELFGHKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAE
                     SEK"
BASE COUNT         3508 a         3784 c         3311 g         3078 t
ORIGIN      
        1 ttacttctgc ttcccgtgat actgctgccc cacacagcga tggcaaacac cctattcgat
       61 atgatcaaaa ccgttggcga tggtgcaaaa ggctccagca gttcatttgt ggccatcgct
      121 caggttattg gcctggtgct ctttattgcc ggcctgcttg gcctcaaaca aatcggtaaa
      181 aaacccggag cagggctggc ctgcatactg tccattgtgg tcggtgcctg tcttgccgtc
      241 gtaccggaaa tgatgagcag tggacagaaa acgctcggtc tttctccggt ctccatcagt
      301 taatccacaa cggcaccggt ttcccggtgc cgtcttcaca caatgaagag gaccagacca
      361 tgtcctgtga gctgccccct ttcaccctgc tcagaacaga gcacggcaac gtgattgccc
      421 gcgacaaccg ggataatcag gagtttgtgt ttgcagaatg ttattccacc gccatcgcag
      481 cactgattct ggctgccgtc accctgtata cagagagaaa aacgtatgaa caaaacaccg
      541 ccactcatcg ccccggcccg ggaacgctgg cttttcccgc tcccgcaacc ggacacggta
      601 ttcagcggtg aatccctgct gccaccaccc gtgctctcaa cacccggacg ctgtccctgc
      661 tgtcgcagga ccgtcactca ccgcttcatt ctggaggatt catggccatt acagcagatg
      721 gctgacacct gccgggatac ggtcgtcctg ctggaaaaaa acctcacccg ggtgatgcgc
      781 ctgaaaaaac atccggtacc ggaaaacgct gacgaaaaaa agaaacacac ccgaaccctg
      841 caggatgcgg agcggtccct ggcacaggcc cgcctgtcgg ccaggcgact ggccctgcgt
      901 catgtggaaa agtcacaaat cgtcacaaca gatgcactca gcgaaaatga aagtgaactg
      961 ctgcagcctg aaggcccgcc ctttcatctc tgtgccttct gccacgcctg gcactgcctg
     1021 aacggctacg ctgccgcaca gggggtgatg gtctggctcc ctgatttgca tccggcctcg
     1081 gtagttgctc tcaatgcacg ggcactgaag gaaattttca gcgatgagcg taagcgaatc
     1141 agacagggac gggccgtact gaatgccctg gtacagaacc gcctggccgt tgaggagaaa
     1201 tttcgcacct ggcgtccggc ggattttgct gatgcactgc gccgctggcc gccggcacag
     1261 cgtaaaacac tgcgggagaa aatggacggg gtggcgctca ttctgatgcc agattcgttt
     1321 cctgacaaaa agtacgtcat gtgacacaca ccgctgccgg tgacgttctg ccggccccaa
     1381 ctgacagagg aacaaaaaat ggataccctc actgccggat atcagttttc cgcagatgca
     1441 ttccggcgct ttattatccg gacgctgact gacgatattc cgcctgacgg agatgaaaaa
     1501 gagattgccc ggttcattct ccgccacccg gcatttaacg gcggctggct gctctttctg
     1561 gctgcagaaa ttcatgaaag atcccgtacc cataacagca tcaccgtcac agtgagcgtc
     1621 gataactatc tctcatggat ggatattgcc gtgtccggtc tcccggaatt accggatatg
     1681 cccttctgtg attttttcat tcgccgccag cagtttatct atccgggtgt acccccgaca
     1741 aaatcaggag aagaatgatg aaaattcacc gcatatcccc ggagacgctt ataacactca
     1801 tcctcgcaca cctggccgga aaagctgaca gtaccgccaa agaagagcac cggctactcc
     1861 gtcggttcct tcgggatgat gacggcaggc tggccggcat actgctgaat attgccggaa
     1921 tacttcagtt caacagggaa ttaagcgccc ggcataatta tccggccaca cccctgactg
     1981 aattttcatt aaggaaacgg ggaaaacaac tacatctctg tctctgcagt ctccgtttct
     2041 tttatatccc ccctgtattt atacgaaatg aaagaagaaa atgtattgtt gtacatatta
     2101 ataaaatcac ttatccgcca gtaaattcac tacgctgaca gcgtgtttcc accttattac
     2161 atttttatta ttaactgtaa tcacacagaa atacgcacct gtccctcaac agattattta
     2221 ttttctctgc tgttttttca tgccacatcc cctgctgtgg catcaaaaaa tatcagaccg
     2281 tgatgattca tccttaccgg agtgctccat gccatgaata tcaaccgcat tatttttgcc
     2341 attgaagact gtatcagtac cctgtcacgg tattccgttt ccgcattctt ccccgattac
     2401 tgtgatctgc acaccgtcat cggtctggac caacaggacc gcgaacgccg cccctggctg
     2461 aaagccccct acattgccac caccaaacag gggtattacc tttccgtctt tgaaatcagc
     2521 ggtgccctga aagaaatgga tgaaaacccg gacctgacag cccccggcac actggaatca
     2581 ctgattacag cgctggcaga gcgaatgaac actgcctata aaaacatcgg tcacaaaatc
     2641 agttttgttt tcgaacgcga tccggagcgt ggaaggaatg aaatcgaagt catgctctta
     2701 ccccagcgaa aatccatcgc acgcacgggg atcctgcttc aggacgttct ggatgaaaaa
     2761 gtcaccacgc tcaccccctg gcttgtccgg gaacgctgct ggctgaccgt ctggagctct
     2821 gaagaactgc tgagccggac cgatcgcaag gaccaccaga cccgtatcag gaaactcgct
     2881 gaacacgctc cgccagcccg atttgcgcag gatccatggc gctggacact ttccgcactc
     2941 aaaatacggc atgacgcctt tctggacacg ctggagcagg ccctgacaca tgattctgat
     3001 ggtctgctgg ttcgcctgat ggatattcat gaagtgggac gggaaatccg ccgtcaactg
     3061 gagcgcaaca gcacacctgc agtatggcag cctcatctgc ctgaagatgc ccgtcctgcc
     3121 ggctggcgac agggcgagga tacctctgta ttccacgcac cgtcgctgaa tctgcagtta
     3181 ttcaccacac agcctgaaac acacggcagc ctgataaagg ccggtgacct ctggcacggt
     3241 atggtcgcca ttaccctgcc cccccagaat ctgcggacat tcaaccagct ggtcagggat
     3301 gtgccccgcg caatcccctg gcgtatccgg atggatctga tgcccggcgg catgaaggcg
     3361 cttggggtca agaaaaccct gctgacctac agcagcttta tttcccctct ccggcccatg
     3421 tatgactcag tgatgatgct gaatgacatc aacaaacatg atccggtctg tatcatgact
     3481 attgttgcca ccacatgggg aaacagccgg gaagtctgta cccgcaacca ggcgctcctg
     3541 aaatctgcgc tggaaggctg gggagtatgc ggcaccacca ccaccttcgg tgatccgaga
     3601 cgtgcctggg tgaacacggt tctggcggcc tcccacggtt ccggtccagt ccccctttac
     3661 ccgccccttt cacacgccct ttccctgttt cccctgaaca gggccggctc tgtctggcgg
     3721 ggacagggaa atctgatgat gcacaccgaa gacggctctg catgggaggt cgcgctggca
     3781 tcgtcacaac agaacaagca cacggaactg acgcccggcg cacccggtct gggcaaatcg
     3841 gtactcatca acgcactgtc ggagatccag atagcctcag cccagaagaa cctgcctttc
     3901 attgcctata tcgacaaggg attcagtgca cagggactgg tacagcttat ccgtgacagc
     3961 ctgccggagc agagaaagga cgaagccgtg gggatcattc tgagcaatga tccggaacac
     4021 acccgtaacc tgtttgatgt gatgtacggt gctaaaaaac ccgccacccc ggaaaagaac
     4081 ttcatggtat ctgtactctg cgcactgtgc gtggataccg gcaccggtca gccctgtaat
     4141 cccggtgaca cccggcaaat cataagcaac ctggtggatc tggccttcag ggagtatggc
     4201 gaaaacaacc ccagactgta ccgtgcagga acagagcccc ttgtggatct cgccctggaa
     4261 gagagtggca tcgcagaaca acatgacgct ggatggtgga atgctgccac ctggtttgaa
     4321 atcagggaca tgctgcatat ggctggcaat atccccgcag cccagcgggc acactaccag
     4381 gccatgcccc tgctggctga aatgtccgcc ctgctgggac aaccatccat ccgggatgtg
     4441 ttcggcacag ttcagcgcga taactcagca gaattgttac tggactacat ccgccgtgcc
     4501 ctcgatcagg gacacagtga ctacccgatg atgtccggct gtacccggtt tatgctcagc
     4561 cccgacaccc gcgttgtggc cgttgatctg aacaatgtgg ccggtgacaa gacacctgcc
     4621 gggcgtctgc gcactggcat catgtacctg ctggcaggac aaatcgccgg cggggatttt
     4681 gtcctgccgc agtatcagga ggaaatacgg aaaaatctcg acccacgcta ccatgaggtg
     4741 attttccgcc gcattgaaca gcttgatcag gaggtcaaga ccaaagtcta tgacgaactg
     4801 cacaatgcaa aagggatcaa tttcatctgg gaagccctgg acacccagga gctggagcag
     4861 cgtaagttcg ggatacgcac agtgctgagc acccagctat tgcaggacta tccaccggca
     4921 gttctcaaat ccgccaacac cctctggttg ctgcgttacc gccctgacga aattccgttt
     4981 ctgcgggata attttggtgt ccctgaagtc accctgcggc gcttcctgaa aatgccggaa
     5041 ggggctgcac cggatggcag cggtgtcccg gtactggctg tgttccgtgt aaaaaacggt
     5101 acgctggccc ggatcctgaa attcactctc ggtccactgg agctgtgggc actgaattcc
     5161 tcacccaaag acagcgccct gagacgtgcg ctgacgcagg agctcgggtc actgcgggca
     5221 cgccagatcc tggcagaaaa ctttccccgt ggttcagcca cctcactgat agaacaccgc
     5281 gcccgtacac atgacagtga aaacgtcatc catgatctgg ctgcagaact catccgtaag
     5341 cagggatatc acctctgatg tacagcatac acatcactcc ccgctgccgg cgtcctgcac
     5401 ggggacaggt cttcctgaaa acgctgcgcg tggacggtga cagtttcagc ttcaccctgc
     5461 cgtccgcctg cttcacgctg cgtccctccc ccaatccccg ggtcattttc gccagtgaca
     5521 gacgggctga cggggaaatg agtggcgttc gcctggctct gcccggagag acaggcacgc
     5581 tgattacaac ggccacctgg caggtcaacg tgccggagcg gcctcctttt gatatacagc
     5641 atgtcattca ctggctcctg ccctcgcctc tcacggcgct gatgtccgac aacacctggc
     5701 tgtggcagga tagccccggc acaccggcac tgacggcggg agactggccg gtaatggaaa
     5761 ccgatacccc gacgctgaaa cgcgtgaagc gtcgtacctt cacgctcatc gataaccgca
     5821 accgtcaggg atggatcacc cgtcgcttcc agtgcttccc gctggctccg ctgccggaat
     5881 cacacagtca ccgcgtacca gacatcctgt atcccctgaa aacggagaaa aaagaatgca
     5941 aagacaacca gcaaccacac tgcttgcact cctgataggc tgtgcggtta tcccgtccac
     6001 ccacgcctat tctgtgaccg tagtggccag ccagcctgtc accacacaaa tcatgccgga
     6061 gctggccgcc attcagggcg tgatggcaga gatggtccgt atgcagacag ccaccggcac
     6121 agccatcaac cagagttcag agaaactggc ctctgtcatt gcgcaggatg ggcaggccac
     6181 ccgtcagcag atgatattca gtaatgaaac ccgccggctt gaggaagccc ggaagagctt
     6241 cagcgtgccg gactccatct gcagtgaatc cgcctccgga gttgcagctg aaagtcgccg
     6301 tgcagcagcg tctgcggctg cacgtctgag tcagggagga ggcgtcagca gtaaaccaat
     6361 ccgtgaacgt ctctcccggg cagcagactc accaatccgc gaggcatatg acagtgcagg
     6421 cattcatgcc ggttactgca cggaagctga atatgcccgc tttggcggaa ccgatgtctg
     6481 ccctgccgtg ggcgagctgc cgggcggaga cagccaggtg cgatcgctct atcagggtgc
     6541 aggcacagca gatacccctg ccgccctcac ctgggatcag aaacagattg atgctgccac
     6601 ggcttacatg aaaaacaccg ccagaccgtc agcgggtcgt gccccgggga aaggtgaagt
     6661 gggaacacag accgggcgca cgtatgtggg cctgcagaat gaatacaacg gcatcatcga
     6721 tgccgcttct catccgcaac tgtcactgat agctgacagt acccctaatg aggccacccg
     6781 tggagcgctg actgaagccc tgcagtcacc atccgctgcg gcatattttg accggaccgc
     6841 atcatcagag gcccgcaccc gtggtcacat gagccagcgg gagtttgagg cctttgaagc
     6901 tggcagacgc tatgcaaaca ccgactggca gcaggatctg cagggcatgg aaggcgataa
     6961 ccttctccgt gaactgctgc gcactaccgc cctgctgaac tggcagatga acgacctgaa
     7021 agagcagata cgccagggta atgttatcgc cgggcagcag ctggcactgg cggcacgaca
     7081 gtattacgga cagcgtctcg gtgaactgtc acaggccatg agccagggga gcgtaagatg
     7141 aaagacgaca acagcaccga caaaaaaaaa ccggcccccc gtctccgccg catcggtgac
     7201 atcgggataa acctgttcgc accactggca gaaacccggt ggatgctgcg cagctgccgc
     7261 catatgctgg gccagcataa aacgcgcctg aagcgcctca tgcccgaccg gcaaccggag
     7321 agtccggtct gtatgacctg ggaagaagcc gtcagggcca gcggccttac ggtcaacgaa
     7381 ctggatcggc gtctgcgacg tcgcaggatg ttctggcgga caggattctg gctgatgatg
     7441 ataccggtac ttctgctgtg cggcatggcc cttgctgcca cagcgcttcc ggccatcacc
     7501 ctgatacgta tactggcctg cctgttactg atgagtgcca ttgcagcact gtgtgcctca
     7561 aaaaccctga ttgtgtcata ccgcctctgg caactgaatg aacggcgggt cagcgaggac
     7621 gaacagggca ccttccccga ttacctgcag gcccggcacg gctggaaaca ggccacatgg
     7681 ctggccatta cccaccggca gtactgaccg tccggtctgc aattcctttt cctcattccg
     7741 gaggctgatg ccctgcatcc cttcggttcc ggagttatcg tgaaaatcat acccagagca
     7801 ctgagtgcgg gtctggcgct ggctgccgta cctgcacagg cattcgtcac ctatcaggac
     7861 attgtgagcg ccgccaccaa tccggatgac ctgtcgcgcc aggcactggt caccattttt
     7921 ggcgacgtgg tcacaaatcc cctgacagcc agcagcaaca ccattatcgg taacctgttc
     7981 atggtgttta acgcaattat cgccattatg gggatagtct ggttcctgtt tattggaatc
     8041 cgccatgttg tccgcaccgg ccaccagggc aatgtcttta acaacggacg ggacgtcgtc
     8101 ggaatgctca gtattgtgag cggttttatg atgattgtgc ccaccgcttc cggctggagc
     8161 ctggcacaac tggtcatgct ctggggcgca tccatcatgg gcattggttc cgccaatatc
     8221 atggttcaga ctgctgcaga aaacatcgct gaaggctatt caatgaccgt acagccagtt
     8281 catgtctcca cccgcacggc agcacgggga atactggaga tggcgctgtg caaacacgcc
     8341 atgaatgcag gcctcgatga ctttaaccgt actgcccgtt catcaacgcc ccccatgagc
     8401 gaatcctcac ggacagagaa cggcaggtat accgtcaccg tttcaaatgg cagtgccacc
     8461 tgcgggagtg tgagtctcac cgtgaaaggg aatggcagca acaaccagtc cgcactggac
     8521 tattttttca atcccttcat tgaaacggag tatcagggtg tcctgacggc acaacgctcc
     8581 gccatggata ccatgctccg tgacctggac agcgcagcac aggaatttgt gtctgcgtac
     8641 cggacccgac gcgacacggg taacggcaac ctgccggata ttgaaagccg tattcagaag
     8701 gctgccaacc ggtatgaaga aacggtgcag aaggcgttgc caacagataa cgcagaaact
     8761 gagcgcaggg agcagctgaa atactacctg accacctacg gctgggttgc cctgggggcc
     8821 tggtaccaga cctttgccac cgccaaccag cgactggcag aactggccga ccgtgccccc
     8881 actgtgagtg gagtttcatc gcagggcgaa gccggagata ctgaattcct gaaggaagta
     8941 aaaggagctt accaggcaca gttgcaaaac tcaccatata cacctgcact gggaacaacc
     9001 gcttcatcaa ctgaatacag aacagcagaa gctgctgaac ctaaaagcat aatcatgaaa
     9061 gtatggtcgg gtgtaaattt tgcagacaaa attgcaacat ccttatctgg aacaggaacc
     9121 acctcagacc aactaaatcc tctgataaaa atgaaaaata tcggtgatta cactatggtc
     9181 acaacagaaa ccatctgggc gggttatacc accgtcaggg tactttccac tgccggcagc
     9241 aactctattt ttggtcgact ggtcaatgcc gttagcggct tatctgatgc cgcaaaggct
     9301 tttctggaag cagctgcacc accgctctac ttcctgctgt ttttgctgtt ctgtgccggt
     9361 ttttcgttat ccatttacct gccgtttatt ccgtttatct tctggatgac cggcatcggt
     9421 aactggatag tcagtgtact gattggatgc gccgccggtc ccctgtgggc tgccacccat
     9481 ctggggacaa accaggaccg gggaagccgg gctgcttacg gttacatcta cctgatagac
     9541 agcatgatca gaccactgtt gatggtgttc gggttctttt ttgcatcggt cgccatcgtg
     9601 gctggcggca ccatactgaa tgcgttgttt ggcgcggctc tggttaatgt gcaggctgac
     9661 tcccttacag ggttattcag tatcgcggga tttttgatga tttatgcccg tgcctgtacc
     9721 acgctggttg cggccgtttt tgcgcttcag gcttatctgc ctgaccatgt aattaatttt
     9781 ctgggcggac gtgacggcgt taatatgctg gggaatatgg tttcatccgt caaagatatt
     9841 attgccggtg gcaacaccaa tatacgcaga acacctggcg ttcgtgaaga aaaactgaaa
     9901 aacattaatg gagatgataa agacggaata aaacaataaa gtacagacat caaaaaaaac
     9961 agtattgcca cattcacctg aagagattaa aatacattca ggtgaatatg gaggtttatt
    10021 atgaacacaa caaaaagcag atatccttcc tggtgggcat attatcaact agcccgcaat
    10081 acttacttta tatttggaat tccatgctta ttattctatg cttttttcgg aacactaatt
    10141 gttcatgact caagcttatc tcaaacaaga gattttattt ttgcatatgg atgctgggca
    10201 ttaataattc tcccattact ctggctatat ctgaaaacca gagcgaagaa aaagaaagtc
    10261 cagcaggttg ttcagcagat aaaagaaagc agtccctttg caccgaccaa cgattacgag
    10321 caactgtcat tctcgaagag ttgttatttc gggatcgaca ttaaaaacgg caccatgctg
    10381 tatgtgcgca tttacccaaa caatgtcatg gatgtgattg gcctggatat tcacaacttc
    10441 acccgaacag ttgctgaaga cggcaagctg gaaatccaca ccacctatgt gagtctgccg
    10501 atgatccctc ttgaaatctc cggcatctca gcaaggacgc tggccaacac catacacacc
    10561 atggctgcac gtggatatga gtacaaagag cgtttcccgc agatgatccg gtatcgggtt
    10621 aaagagtggg aaaaagttgc cggtgttcct gttgcagaag tattttgagg tagctatcaa
    10681 ctgttagacg ataatccgtc agccggtttt ctctgaaccg gctttttctt ttcaggcaga
    10741 atgcctgtct gccgtccctg tatcagactg taattagcac aatacggcaa tttgccgttc
    10801 agtatattca ggaagtacaa atcatgccat ctgcaacaca aactgcccga ctggaagcaa
    10861 ggatcagcac agaactgcat gctatgctga aacgcgctgc agaaatccag gtaaaaaagg
    10921 gcgcattgag gtttgctttc gcaacaatcc ggcggcagat ttcctgccgc cgtgttgccc
    10981 attaacgttt aacttcgtag gctaacgttg ccacaagcac tgtgtttccc tgctcgacac
    11041 ggaatccgca aagcgaatcc ctcaccatcc agacaaaaca cagtatcgtc acacagacga
    11101 caattaacat tgttaaaaac gttcgttgtg gcatcgcagc ccctccattg cctttcggcc
    11161 gtcatcgggc tactataatg ttgtctaggc attacagtgg gccctgggtt gattgataaa
    11221 aaaatcgcct ggggctttct gctgcatgcc gtccacaaaa cgctttcagc atacagcctc
    11281 aagcgcccgc cgctattcta atcgattttc agtctgccac aatcgttttc cacctaatga
    11341 catgttatgc acaacagtat ttttacggga aactacacgg caaggattcc cggaacacgt
    11401 tcattatttg atggttacag acatcatgtt aaatccggaa gtaataatga cctgaaagga
    11461 aatactatga gtaacgaata tcaaagcacc agtataaacc ctgatgtttt tgaacaattt
    11521 cttaccctcc tgaatacaca ctgtaaatca tcagaagctg cacttgccat gctgagtaca
    11581 ctctgctacg aactgatgag gaggagtggg atccattcac tggacagcct gatgctcaac
    11641 ggagaaggta tattgataca attacagggg gctgatattc gcgaaaaaac aggtcagcat
    11701 attcactgat ccagggatgt gctgaaaaca gtgtcagcct gaaataaacg ttcaatgcct
    11761 catacacatg aggcattgag cacggtcatc aataatcttt attatttttc tgattcagca
    11821 tttccggtca gtatcggctc ttctggcaaa gacattaccg gaacgtcacg ttcaccattg
    11881 aatacagaaa cactttcagc gaaatcattc tgtaacaaag aaaaacttaa cggcagattt
    11941 ttggtttgta tccctgtgat ccattgaaaa atctcgggaa aacgacgtgc actgatatgc
    12001 acatgattga ggtggtgctc agtaatatca ctggccatca tcatgagagg tacatcgtat
    12061 cccgaacgca aacctggcgc atgatgcaca ggggtgctgt ctgaactgac ggattgccca
    12121 tgatcagaaa aatacagcat ggcaaaatca tcaccatgct ctgtcgtcat gttataaatt
    12181 gcctgaagaa accgatccag tttaagcagt gttgataaat aacaggatac tttatgtccg
    12241 aataattccc tgaatggctg tgcatcatta aaaagtcggt cacagggatt agtgtgagac
    12301 cccagcatat gaataaaaat cacttttgga gaagacgagt taacagcttc cctgacatac
    12361 tgaagcatat cctcatcatc aatgcgaagg gaagaagagc tcccttcctt aagaaaatgt
    12421 tttttatccg cattggcggc gatgacagaa atcagcgtat catgttgccc taaagagccc
    12481 tgatttgata tccaccacgt ctcatacccg gcttttctgg caagcgtaac aatattatta
    12541 cccggattac ctgtttcagt gtaatcaagc gtcagtgttc tgctgagtga acctatcgta
    12601 ttcggcgctg ctgagaaata cccattaata aatatacccg gagcagaatt cagccatggt
    12661 gttgtcggca aaggataccc ataaacagaa agataatccc gtctgacact ttcaccaatt
    12721 acgataatta tggttttata tttcttctcc acactcacaa tatcccatga gtctttctga
    12781 ttgtttttca gcgattcata tcgcatcatt tcttctctgg tttcagctat ccctgtcatt
    12841 acattcgtaa ccatacgttt tggccatgca tttactgaca atacgataaa gaacaacaga
    12901 aagataaatc cccggtggct tttatcacgc ttacgccaga accagaatgt aaaagcagca
    12961 atgaccagcg aaacaataaa ataaagaaca ggtatgcctg tgataaactc acctgcctca
    13021 tttatatttg tctgcattac agacgcaata aaactgttgt ttattttccc gtaaatcatc
    13081 ccaaccggag catagaatgc acttaacaaa agaaagatac agacacacgt tgctgaaata
    13141 acattccgcc gccccaacaa catcatcagg aacaatataa gcacattccc cagggcgttt
    13201 ttcactgtat atccgcagca ccatgtaaca agtacagaaa acaacagata aatcagtgac
    13261 tctttacacc acaacataaa ataaaactgt tttgctctct caaaagcata tttcgacttt
    13321 tctttaatac aagacacgcc agtcattttc ctgtacaaac acagacgatc atttcaacgc
    13381 atggatattt atatgaaaca gttcacaaaa ataccctacc ctgtccagca gggtttacat
    13441 attatcaggt tctgtgttta cagcaactgc tccccgaacc agattattcg cataacccga
    13501 tgaaggcctc ataaccatca aacactcaga atacatttag ttaacaaaaa tatgtcttgc
    13561 cctcccggaa aggcaaacat acgaaagaga aagcatggct ataataatat caatcaaaat
    13621 atgctcaatc tttccggttg ccatacactc agcaccgaca acataaagac aataaacaaa
    13681 a
//
LOCUS       DAZKGF010000133        13662 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZKGF010000133 DAZKGF010000000
VERSION     DAZKGF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.133"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(70..1428)
                     /gene="plaP"
                     /locus_tag="ACHOQV_003712"
     CDS             complement(70..1428)
                     /gene="plaP"
                     /locus_tag="ACHOQV_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFP9632010.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1418..1480)
                     /gene="yoeI"
                     /locus_tag="ACHOQV_003713"
     CDS             complement(1418..1480)
                     /gene="yoeI"
                     /locus_tag="ACHOQV_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFP9632011.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1695..2624)
                     /gene="yeeY"
                     /locus_tag="ACHOQV_003714"
     CDS             complement(1695..2624)
                     /gene="yeeY"
                     /locus_tag="ACHOQV_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9632012.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2670..3494)
                     /gene="yeeZ"
                     /locus_tag="ACHOQV_003715"
     CDS             complement(2670..3494)
                     /gene="yeeZ"
                     /locus_tag="ACHOQV_003715"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9632013.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3577..3831)
                     /gene="yoeB"
                     /locus_tag="ACHOQV_003716"
     CDS             complement(3577..3831)
                     /gene="yoeB"
                     /locus_tag="ACHOQV_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFP9632014.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3828..4079)
                     /gene="yefM"
                     /locus_tag="ACHOQV_003717"
     CDS             complement(3828..4079)
                     /gene="yefM"
                     /locus_tag="ACHOQV_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFP9632015.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4362..4412
                     /gene="hisL"
                     /locus_tag="ACHOQV_003718"
     CDS             4362..4412
                     /gene="hisL"
                     /locus_tag="ACHOQV_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFP9632016.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4388..4511
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4558..5457
                     /gene="hisG"
                     /locus_tag="ACHOQV_003719"
     CDS             4558..5457
                     /gene="hisG"
                     /locus_tag="ACHOQV_003719"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFP9632017.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5463..6767
                     /gene="hisD"
                     /locus_tag="ACHOQV_003720"
     CDS             5463..6767
                     /gene="hisD"
                     /locus_tag="ACHOQV_003720"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFP9632018.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6764..7834
                     /gene="hisC"
                     /locus_tag="ACHOQV_003721"
     CDS             6764..7834
                     /gene="hisC"
                     /locus_tag="ACHOQV_003721"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFP9632019.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7834..8901
                     /gene="hisB"
                     /locus_tag="ACHOQV_003722"
     CDS             7834..8901
                     /gene="hisB"
                     /locus_tag="ACHOQV_003722"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFP9632020.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDLANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8901..9491
                     /gene="hisH"
                     /locus_tag="ACHOQV_003723"
     CDS             8901..9491
                     /gene="hisH"
                     /locus_tag="ACHOQV_003723"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFP9632021.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLARRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9491..10228
                     /gene="hisA"
                     /locus_tag="ACHOQV_003724"
     CDS             9491..10228
                     /gene="hisA"
                     /locus_tag="ACHOQV_003724"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFP9632022.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPARRQIPLIKTLVADVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10210..10986
                     /gene="hisF"
                     /locus_tag="ACHOQV_003725"
     CDS             10210..10986
                     /gene="hisF"
                     /locus_tag="ACHOQV_003725"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFP9632023.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10980..11591
                     /gene="hisIE"
                     /locus_tag="ACHOQV_003726"
     CDS             10980..11591
                     /gene="hisIE"
                     /locus_tag="ACHOQV_003726"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFP9632024.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11686..12663)
                     /gene="wzzB"
                     /locus_tag="ACHOQV_003727"
     CDS             complement(11686..12663)
                     /gene="wzzB"
                     /locus_tag="ACHOQV_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFP9632025.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12810..>13662)
                     /locus_tag="ACHOQV_003728"
     CDS             complement(12810..>13662)
                     /locus_tag="ACHOQV_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFP9632026.1"
                     /translation="EINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKAL
                     YDNLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMR
                     VAYFNELDSYAESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANY
                     QSVPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMK
                     RIKAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYT
                     RDLFGSD"
BASE COUNT         3422 a         3568 c         3495 g         3177 t
ORIGIN      
        1 aaaaagccgg agcgacaaac tccggctttt tgagatttac cggatgatta tgtcatccgg
       61 tttgtaagat tacgctacgt cttcgtactg cggaaccggg ttacggaaac tcttggttac
      121 gcaagccagg taaatcagac cgatagcagc ccagatcaga cccagaacca ttgagctttc
      181 ttccaggtta acccacagcg caccaacggt cagcgcacca cacatcggca ggaacagata
      241 ctggaagtga tctttcagcg tcttgttacg cttctcacgg atccagaact gcgagatgac
      301 cgacaggtta acgaaggtga acgccaccaa cgcaccaaag ttaatcagcg ccgtagccat
      361 taccaggtcg aagttgattg ccagcagggc aatcgcgcca accaggatga tgttcatcgc
      421 cggagtacgc catttcgggt gtacataacc gaagaagctt ttcgggaaca cgccgtcacg
      481 acccattacg tacatcagac gcgctacgcc tgcgtgcgcc gccataccgg acgccagtac
      541 ggtaatggtg gagaagatca gcgcgccaac ctggaacgct ttacccgcaa cgtacagcat
      601 gatttcaggc tgtgacgcat ccggatcttt aaagcgagag atatccggga agtacagctg
      661 caggaagtaa gttgcaaaga tgaagatcat gccgccgatc agcgcggtca ggaaaatcgc
      721 acgcgggatc acgcgctctg catctttggt ttcttccgac aggttgctga tgccgtcaaa
      781 accggtaaag gagaagcaca ggattgtcgc cccggtaatc atcgggataa catgcgcatc
      841 gccagaccag aacggacgag tgctcgccag cgtaccagcg ccttcacctt caaatacgcc
      901 ataaacaacc atgcccagaa tcaccgcgat cagcactacc tgcaacacga cgattacagt
      961 gttgaagttc gctacggatt tcagactacg caggttaaag gcggtcataa aggccaccag
     1021 cgccaccaca aacatccacg atggaatgga aggcaccaga gcttcaaaat agattttcgc
     1081 cagcagaatg ttgatcatcg gcgcgaacag atagtcgagc agagaagacc aacccaccat
     1141 aaagccgaca gtcgggctaa tggatttctg ggcgtaagtg tatgcagagc cagcagaagg
     1201 atagcggcga accagcttcc cgtagctcag agccgtaaac aggatcgcaa tcaatgcgaa
     1261 cgcataggct gtcggcacat gaccatccgt gaggcctgaa acgataccaa aagtatcaaa
     1321 cagcgtcatc ggctgcatat aggctagacc catcattaca accggaacta acgtaagcgt
     1381 tttacgcaat tccacgcgag aggtgtttgg agtaacgtta tgcgacatgg tcattctcct
     1441 ttacggtgat aaccgtcgcg taagcaaaaa attgccccat ttttttggat tcctcagcga
     1501 caacaactgt cgatttttag taaatatcta tccggtacga agcccggcct cttggtatga
     1561 atgattggtt tgaagcaaaa aataaccgac gctaattaaa cgtcggtttt tagtcatttt
     1621 ttgacagcgg cgcattgtgc ctaaatgggg gggaaatgac aagagaatga gaggcttgtc
     1681 ataataattt ttctttaaat ggctgatttt ccgtcatcag attttgcata aacaccggcg
     1741 atcgcactat ttgctaaaat ttcgtcccgc caccaaccac tggcaagccc tgcggtctgt
     1801 tcattccagc caatccagac gggttcaaag agagtttggg cgaccacttt cttctcgatt
     1861 aacgcgccac tatcgagaaa acgttgcgcc agataccgtg gtaaatagcc gcagccaagg
     1921 ccgctaattt gcagctccag cttggtttta aaatcgaaaa cggtaatggc ttcttgttcg
     1981 tcgagaagtt gtgaggccgt agaagcgcct gcctgcgcag tgtcccccac cacaatcgcc
     2041 cgatatcgtt taatgatacg acggtttaac ggttcttctt cctgggctaa cggatgatga
     2101 ggcgcgaccg caaagacttg ttcaagatcg cccaaccgcg agaaaccaaa ctcactggaa
     2161 gatggtggct catgcatcgc gccaacaata atatccgctc gcccctgagt cagtgcatcc
     2221 caggaaccac cgagtacgcc attgataaac ttcaggcgcg tcacgctatg atgttgataa
     2281 aaagcctcaa tgagaggcgc aagcagagaa aaaggaaacg tgtcgtccac gccaatcacc
     2341 agctcatttt cccagccttc atgcagtttg atagcctgct tttccagttc tcgcacggta
     2401 tgcagaactt cccgcccttt ctctaataac atttttccgg tgcgggtgaa tttagcgcgg
     2461 tgaccgctac gatcaagcaa ttgaatatta aggtcgcttt ccagcttgtg aacggtataa
     2521 ctcagagcgg acggcgtctt gtagagttta gccgacgccg ccgcaaaact gccttctttt
     2581 tctaaggcat cgaggatcat caacacatcc agcagtggtt tcatacacgc ccccttgcag
     2641 tgtatgcggc gtcctgctgg cgtgatcgtt tactccagcg gcattaccag cggatcggga
     2701 tactggtatt caaatcccag ttcattacaa atccgactgc catcaataat cttacctttg
     2761 ccgctgtcca gactatttct gaactgcgga ggttccagcc ccagtaaacg ggccatctgc
     2821 ggatagaaaa cattacgcgc agggtgagct ggcgcacata tattatagat gtgtccgcct
     2881 ttaggtgcct gtaacaacag agtgatagca ccaataacat cttctaaatg gactaaatta
     2941 acaccatgtt caccatcagg ggcggttttt ccggcaaaga agcgtccggg atgacgtccc
     3001 ggtccgacca gccccgcaag acgcagaata tcgaccgaag taccgggtaa attgtgcagc
     3061 cagtcttcga gttcttctaa tactcgtcca ctgttggtca ccggattacg cggggtggtt
     3121 tctttcaccg tgccttgcgc gtcgccatag acagatgttg agctggtaaa aataattcgg
     3181 ggaatacgat gggccagcgc gctatccact aactcttgta ccgcttgtaa atagaactca
     3241 tcgccggggc cgctacgacg tgccggaagc gtaatgacca gcgcgtcggc atccatcagg
     3301 gcatccagat cgtcagaatc gcaaactaac tcaggctcca tgcgaagcag atagctatca
     3361 atgccactca ttcgggccgc ttcgacacca tcttgtgtgg ttttactccc ggtgacttgc
     3421 cagcctcgcg ctgaaagtga catcgccagc ggcatgccta accaccctaa cccgacaatt
     3481 gcgacctttt tcatccgtta tctcctgata caccttagat ctataaggct acgctagcgt
     3541 atcaaaactg acaattcatt ctatgaatga atctgttcaa taatgataac gacatgctgc
     3601 aatgagtagt gaatcgtcgg taaccgcgta taccagacgg tgctcctctg taatgcgtcg
     3661 ggaccagaaa cctgacaaat tatgtttcag gggttctggt ttccccttac cttcaaatgg
     3721 tgttctgcgg gtatctttga taagttcatt gatcttttta acaattcgct tatctgtttc
     3781 ctgccagtac agataatcat cccatgattc ctcagaccag attagtttca ctcaatgatg
     3841 tccttttccg ttcctttgcc agatttcagg ctatcgattg agtccatcaa tctccgggcg
     3901 ttagcggggg agcgcagtag ataagccgtc tcctccaggg agttgtattc ttcgagtgac
     3961 atcagaacac aagcctctcc attctgacga gtaataagga tcggggcatg atcttcaacg
     4021 gctttcatca ttgttgccga caaattctga cgcgcttcgc tgtagctaat tgtacgcatg
     4081 tcaatctcct cttttgtaca gttcattgta caatgatggg cgttaattaa ctatttatta
     4141 attagtttgt agatcaaggt attgtcagtg agacgaaaat ccaggcttcg ctatttttgg
     4201 tgccgtcagc ttagaggaca gtcctcttag ccccctcctt tccccgctca ttcattaaac
     4261 aaatccattg ccataaaata tataaaaaag cccttgcttt ctaacgtgaa agtggtttag
     4321 gttaaaagac atcagttgaa taaacattca cagagacttt tatgacacgc gttcaattta
     4381 aacaccacca tcatcaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
     4441 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
     4501 ggctttttta ttgcgcggtt gataacggtt cagacaggtt taaagaggaa taagaaaatg
     4561 acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
     4621 cgcgaattgc tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg
     4681 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgacgacat tcccggtctg
     4741 gtaatggacg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg
     4801 cttaaccgcc gagcccaggg tgaagatccg cgctacttta ccctgcgtcg tctggatttc
     4861 ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggatgg cccgctctcc
     4921 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
     4981 aaaggcatct cttttaaatc ctgcttactg aacggttctg ttgaagtcgc cccgcgtgcc
     5041 ggactggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agcaaacggc
     5101 ctgcgcgaag tcgaagttat ctatcgctcg aaagcttgcc tgatacaacg cgatggcgaa
     5161 atggaagaat ccaaacagca gctgatcgac aaactgctga cccgtattca gggcgtgatc
     5221 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgcct ggatgaagtc
     5281 atcgccctgc tgccaggtgc cgaacgccca actattctgc cgctggcagg tgatcaacag
     5341 cgcgtagcga tgcacatggt gagtagcgaa accctgttct gggaaacgat ggaaaaactg
     5401 aaagcgctgg gtgccagttc aatcctggtc ctgccgattg aaaaaatgat ggagtgatcg
     5461 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
     5521 tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt aacgatatcc
     5581 tcgataacgt gaaagcacgc ggcgatgagg ccctgcgtga atacagcgcg aaatttgata
     5641 aaaccacggt taccgcgctg aaggtgtctg ctgaggagat cgccgccgcc agcgaacgcc
     5701 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gaatattgaa accttccaca
     5761 ctgcgcaaaa actgccgccg gtagatgtcg aaacgcagcc aggcgtgcgt tgccagcaag
     5821 tcacgcgccc ggtcgcttca gttgggttgt atattcctgg cggctccgcc ccgctcttct
     5881 caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
     5941 gctcaccgcc gccgattgcc gacgaaattc tttacgcggc gcagctgtgc ggtgtgcagg
     6001 atgtgtttaa cgtcggcggc gcacaggcca ttgccgcact ggcgtttggt acggaatctg
     6061 tgccgaaagt tgacaaaatc ttcgggccag gtaacgcctt tgtcaccgag gcaaaacgtc
     6121 aggtgagcca gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc
     6181 tggtgattgc tgacagcggc gctacgccgg atttcgtggc ttctgatttg ctttctcagg
     6241 ctgaacacgg cccggactcg caggtgattt tactgacgcc cgatgctgat atggcgcgtc
     6301 gcgttgccga ggctgtcgaa cgccaactgg cagaactgcc gcgtgccgaa accgcccgcc
     6361 aggcactgaa cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtagagatct
     6421 ccaaccagta cggcccggag cacctgatca tccagacccg caacgcccgc gatctggtcg
     6481 atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcacccgaa tcggcgggtg
     6541 attacgcctc cggcaccaac cacgtactgc cgacttacgg ttacaccgcc acctgttcca
     6601 gcctcgggct ggcagatttc cagaagcgta tgaccgtgca ggaactgtcg aaagaaggtt
     6661 tctccgcgct ggcttcgacc atagaaacac tggccgccgc cgagcgcctg accgcccata
     6721 aaaatgccgt tactttgcgt gttaacgccc ttaaggagca agcatgagca ccgtgactat
     6781 taccgattta gcgcgtgaaa acgtccgcaa cctgacgccg tatcagtcgg cgcgtcgtct
     6841 gggcggtaac ggcgacgtct ggctgaacgc caacgaatac cccacagccg tggagtttca
     6901 gctcactcaa caaacgctca accgctatcc ggaatgtcag ccaaaagcgg tgattgaaaa
     6961 ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg gcgcggacga
     7021 aggtattgaa ctgctgattc gcgctttttg cgaaccgggt aaagacgcca tcctctactg
     7081 cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggcgtcg agtgccgcac
     7141 agtgccgacg ctggacaact ggcaactgga cttacagggc atttccgaca agctggacgg
     7201 cgtaaaagtg gtctatgttt gcagccccaa caacccgacc gggcaactga tcaacccgca
     7261 ggattttcgc actctgctgg agttaacccg cggtaaagcg attgtggttg ccgatgaagc
     7321 ctatatcgag ttttgcccgc aggcatcgct ggctggctgg ctggcggaat atccgcacct
     7381 ggctattttg cgcacactgt cgaaagcttt tgctctggcg ggccttcgtt gcggatttac
     7441 gctggcaaac gaagaagtca tcaaccttct gatgaaagtg atcgccccct acccgctctc
     7501 gacgccggtc gccgacattg cggcccaggc gttaagcccg caggggatcg tcgccatgcg
     7561 cgaacgggtg gcgcaaatta ttgcagaacg cgaatatctg attgccgcat tgaaagaaat
     7621 ctcctgcgtg gagcaggttt ttgactctga aaccaactac attctggcgc gctttaaagc
     7681 ctccagcgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
     7741 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
     7801 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
     7861 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa
     7921 ctcgcctttg aaccgggcgt gatcccggag ctgctgaagc tgcaaaaagc gggatataag
     7981 ctggtgatga tcaccaatca ggatggtctg ggaacacaaa gtttcccgca ggcggatttt
     8041 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa
     8101 gtgctgattt gcccgcacct acccgccgat gagtgcgact gccgtaaacc gaaagtaaaa
     8161 ctggtggagc gttatctcgc tgagcaagcg atggatctcg ccaacagtta tgtgattggc
     8221 gatcgcgcga ccgatattca actggcggaa aacatgggta ttaatggttt acgctacgac
     8281 cgcgaaaccc tgaactggcc gatgattggc gagcaactca ctaaacgaga ccgttacgcc
     8341 catgtagtgc gcaacaccaa agagacgcag attgacgttc aggtatggct ggatcgcgaa
     8401 ggtggcagca agattaacac cggcgttggc ttctttgatc atatgctgga tcagatcgcc
     8461 actcacggcg gtttccgcat ggaaatcaac gtcaaaggcg acctctatat cgacgatcac
     8521 cacaccgtcg aagataccgg cctggcgctg ggtgaagcgt taaaaattgc cctcggcgat
     8581 aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgt
     8641 gcactggata tctctggtcg cccacacctg gaatataaag ctgaatttac ctaccagcgc
     8701 gtcggcgatc tcagcaccga gatgatcgag cacttcttcc gttcgctctc ttacaccatg
     8761 ggcgtgacgt tgcacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agagagtctc
     8821 tttaaagcct ttggtcgcac cctgcgccag gccatccgcg tggaaggcga caccctgccc
     8881 tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga taccggctgc gccaatttga
     8941 actcggtgaa gtcagccatt gcgcgtcacg gttatgaacc caaagtcagc cgtgacccgg
     9001 acgtcgtgtt gctggccgat aaactatttt tacccggcgt aggcaccgca caagcagcga
     9061 tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc
     9121 tgggcatctg cttagggatg caactgctgg cgcggcgcag cgaagagagc aacggcgtcg
     9181 atttgctggg cattatcgac gaagacgtgc cgaaaatgac cgactttggt ctgcccctgc
     9241 cgcatatggg ctggaaccgc gtttacccgc aggcaggcaa ccgcctgttt caggggattg
     9301 aagacggcgc gtacttttac tttgttcaca gctacgcaat gccagtcaat ccgtggacca
     9361 tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
     9421 gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagctgctg aaaaacttcc
     9481 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca
     9541 tcagggcgat tatggcaaac aacgcgatta cggtaacgac ccgctgccac gcttacagga
     9601 ttacgccgcg cagggtgccg aagtgttgca tctggtggat ctgaccgggg caaaagatcc
     9661 ggctagacgt caaatcccgc tgattaaaac cctggtggcg gacgttaacg ttccggtgca
     9721 ggttggcggc ggcgtgcgta gcgaagaaga cgtggcggcg ttactggaag cgggcgttgc
     9781 gcgcgtggtg gttggctcca ccgcggtgaa atcaccagaa atggtgaaag gctggtttga
     9841 acgtttcggt gccgatgcct tagtgctggc gctggatgtc cgtattgacg agcaaggcaa
     9901 caagcaggtg gcggtgagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt
     9961 ggaaacctat cttcccgtcg gcctgaaaca tgtgctatgt accgatatct cgcgcgacgg
    10021 cacgctggca ggttctaacg tctctttata tgaagaagtg tgcgccagat acccgcaggt
    10081 ggcatttcag tcctccggcg gcattggcga cattaacgat gttgctgccc tgcgaggtac
    10141 tggcgtgcgc ggcgtaatag ttggtcgggc attactggaa ggtaaattca ccgtgaagga
    10201 ggctatcgca tgctggcaaa acgcataatt ccatgtctcg acgttcgtga tggtcaggtg
    10261 gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc gctggcaaaa
    10321 cgttacgccg aagaaggtgc agacgaactg gtgttctacg atatcaccgc ttccagcgat
    10381 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtaatcga cattccgttt
    10441 tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg
    10501 gataaaatct ccattaactc ccctgcactg gcagacccga cgttaattac tcgcctggcg
    10561 gatcgctttg gcgtgcagtg tattgtggtc ggcattgata cctggtacga cgccgaaacc
    10621 ggtaaatatc atgtgaatca atataccggc gatgaaagcc gcacccgcgt cactcagtgg
    10681 gaaacactcg actgggtgca ggaagtgcaa aaacgcggtg ccggagaaat cgtcctcaac
    10741 atgatgaatc aggacggcgt gcgtaacggt tacgacctcg aacagctgaa aaaagtgcgt
    10801 gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctg
    10861 gaagccttcc gcgatgccga cgttgacggt gcgctggctg cttccgtatt ccacaaacaa
    10921 ataatcaata ttggtgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
    10981 tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacgga ctgatgccgg
    11041 tgattgtgca acatgcggta tccggcgaag tgctaatgct gggctatatg aacccggaag
    11101 ctctggacaa aaccctcgaa agcggcaaag tcaccttctt ctcgcgcact aaacagcgac
    11161 tgtggaccaa aggcgaaacg tcgggcaatt ttctcaatgt ggtgagtatt gccccggact
    11221 gcgacaacga cacgttactg gtgctggcga atcccatcgg gccaacctgc cacaaaggca
    11281 ccagcagctg cttcggcgac accgctcacc agtggctgtt cctgtatcaa ctggaacaac
    11341 tgctcgccga gcgcaaatct gccgatccgg aaacctccta caccgccaaa ctgtatgcca
    11401 gcggcaccaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag
    11461 caacggtaca tgaccgcttt gaactgacca acgaggcatc tgatttgatg tatcacctgc
    11521 tggtgctgtt gcaggatcag gatctggatt taacgacggt aattgagaac ctgcgtaaac
    11581 ggcatcagtg agttgcgggt gatcagatgc gatgttgttg tcgcatccgg caaaaaaaac
    11641 gggcaaggtg tcaccaccct gcccgttttc tttaaaacca aaagattact tcgcgttgta
    11701 attacgcaga gcgttacgcc ccagcacaat ccccgcgcca accatgccgc ccagcagcac
    11761 tgccagaatc aaggtaattg cctttttcgg gctatcgcga cgaataggta acgtcggttt
    11821 catcacatag cggtaagcat gaatatcaag atcatcaacc tttaagtttt caatatccaa
    11881 caggttttga cgagtctgat agtagtttgg cgagaacacc aacggacgag tcgcctcatg
    11941 cttaatcatc gactccagcg cttcgctccc caaaaggaac aacgtatctt gtgtcacatc
    12001 ttcagtctgc tgaacctgtg gctttgtcac ctgcgcctga ttcgcatact gcaacgcttc
    12061 ctgaatctga cggatacgca gatctttctg ctcttgcgcg actacttcct gagttcttaa
    12121 agagtcctgc aagtttttca tacgcagcgc gatgttgtct ttcaggtctt tttccagttc
    12181 ctcattaacc tggtcgtcaa cttgctggat atacttcgcc agctgcttct gagcaccttc
    12241 aggtgtttca ccaacataag acaccgctaa aggtaatgac tgatttttaa cggtaggctc
    12301 aatggtcagc ttttctgctt cttcctgatt atccagcgtt tccgctaatg ctgaaaatgc
    12361 agtgctgtaa cgtccgataa gtgaagcctg aatgtcggag actttcggta cagccgatcc
    12421 gtagatcaca ttcatcgcgt tggtataacc agcgatttgc cccacatcag gttgagtaac
    12481 gatagctgtg gaagtccact tttctttggc aacggccaga taccctatag ccagggcaat
    12541 agcaacaatg acagaaatga tgatagtcat cttgccgcgc cacaactgca ccagtaaatc
    12601 aatcaaatca atctgttccg ggtcatggtt ttgcccagaa acattattat tttctactct
    12661 catcattacc ctaacggacg aaataatttt aagtcgccat agtttagcga taatcgccat
    12721 gaatgctata ggataaatga taaaaagaat gaattattac aaagggaaca tgagagaatc
    12781 agggcgaaaa tcgccctgat aacaggatgt tagtcactgc caaagagatc gcgggtgtag
    12841 actttgtctg ccacatcctt aagctcttct gccatacggt tggaaataat aacgtcggct
    12901 tgttgtttga aggtggcgag atcacgttcc agtcgagagt tgaagaatga atcttctttc
    12961 atcaccggct cgtagatgat cacttcaaca ccttttgcct tgatacgttt cataatcccc
    13021 tgaatggaag acgcgcggaa gttatctgac ccgctcttca taatcagacg ataaataccc
    13081 accacttgcg gtttgcgtga caaaatggca tcggcaataa aatctttacg cgtgcggttt
    13141 gcatcgacaa ttgccgagat caggttattc ggcacagact ggtagttcgc cagtaactgc
    13201 ttggtatctt tcggtagaca ataaccacca tagccaaacg acggattgtt gtaatggttg
    13261 ccaatacgcg gatcgagaca aacaccttcg ataatctgac gagtattcag acctaaactt
    13321 tctgcatagc tatccagctc gttaaagtat gctacgcgca tcgccagata ggtattagca
    13381 aacagtttaa tcgcttctgc ttcagtggag tcggtaaaca gggtcgggat attttgctta
    13441 atcgcgcctt cctgtaataa cgccgcaaaa cgttctgcgc gttctgaacg ctcaccgatg
    13501 acaatacgtg acggatgaag gttatcgtaa agagctttac cttcacggag aaattccgga
    13561 gagaaaataa tattttcagt acgatatttc ttatgcatcg cttcggtaaa accaacggga
    13621 accgttgatt tgatgaccat aaccgcataa ggatttatct ca
//
LOCUS       DAZKGF010000134        13616 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZKGF010000134 DAZKGF010000000
VERSION     DAZKGF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.134"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(195..815)
                     /locus_tag="ACHOQV_003729"
     CDS             complement(195..815)
                     /locus_tag="ACHOQV_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP9632027.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAVFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            complement(1081..1746)
                     /locus_tag="ACHOQV_003730"
     CDS             complement(1081..1746)
                     /locus_tag="ACHOQV_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFP9632028.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(1887..2528)
                     /locus_tag="ACHOQV_003731"
     CDS             complement(1887..2528)
                     /locus_tag="ACHOQV_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFP9632029.1"
                     /translation="MCLSELQAGLNEGQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            3231..4103
                     /gene="repA"
                     /locus_tag="ACHOQV_003732"
     CDS             3231..4103
                     /gene="repA"
                     /locus_tag="ACHOQV_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFP9632030.1"
                     /translation="MAALNQTSSTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAVYVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALF
                     AALDISEEAVASARRSRVEWENRQRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            5010..5279
                     /gene="yacA"
                     /locus_tag="ACHOQV_003733"
     CDS             5010..5279
                     /gene="yacA"
                     /locus_tag="ACHOQV_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFP9632031.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDKMSRKVAGK"
     gene            5279..5557
                     /gene="yacB"
                     /locus_tag="ACHOQV_003734"
     CDS             5279..5557
                     /gene="yacB"
                     /locus_tag="ACHOQV_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFP9632032.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            5603..6451
                     /locus_tag="ACHOQV_003735"
     CDS             5603..6451
                     /locus_tag="ACHOQV_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9632033.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQLLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGGRPVIIFNARFDIRILKQTAAAHSDPAYWLEEMTVYCAMEL
                     AAGYYGATNRYGTISLASAASQAGLTWEGLAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLEI"
     gene            6592..7050
                     /locus_tag="ACHOQV_003736"
     CDS             6592..7050
                     /locus_tag="ACHOQV_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632034.1"
                     /translation="MRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQRASRLAASV
                     KRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQHR
                     SADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKKEVG"
     gene            complement(7414..7839)
                     /gene="nudI"
                     /locus_tag="ACHOQV_003737"
     CDS             complement(7414..7839)
                     /gene="nudI"
                     /locus_tag="ACHOQV_003737"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP9632035.1"
                     /translation="MRQRVIVCPVIQNEGEFLLCKMASDRGVFPGQWALPGGGMEPGE
                     TMETALRREIKEELGKELHITDVKPWAFRDDIRTKTYPDGTHEDIYMIYLIFDCISTN
                     RTVTFNEEFQDIVWASPDTLKYMDLNEATRITFSQKGIL"
     gene            complement(7841..8548)
                     /locus_tag="ACHOQV_003738"
     CDS             complement(7841..8548)
                     /locus_tag="ACHOQV_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014727861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9632036.1"
                     /translation="MRYNYLYMDPWSADKKEKNKTRNPLYICLSGNSGVGKSTLLRRM
                     SKELFKVDPDTVAIDEKSVHHTLLPYLFDDTSNYGYLIQLNFMIQRALLIKSWLDKGF
                     NLIMERSHIEDYIFINFMLKSEYINKAQYDAYMVLWSRINELLTEPDIIAFINYPIAH
                     SLMHLNDDEIHGVRPEEFPDEEMKIKWITGWHAEYQHFTDNLPLHLRERIMVCAHPDD
                     IDDFSDKVIQKAMMLRT"
     gene            complement(8545..9411)
                     /locus_tag="ACHOQV_003739"
     CDS             complement(8545..9411)
                     /locus_tag="ACHOQV_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP9632037.1"
                     /translation="MDISTLLLDFRITSHCNLGCDLCFRTPGIQDSSLASARNVIERM
                     YKMGFRRIGFTGGEPTSRSDFIDLIEYAKQLGFMTYLSTVGHRFIMDHARLNPVLDWV
                     GIPIDGIDSLVNSEIRSDKMSNQHRVIKNILTWLPQNNNRINIKLTTVVSQSNINHLS
                     DIVCWVDSLPYKIQAWRFYQFCPLGVGKEKRDKLEINTDIFKDKMVMLKKQHPNMPIS
                     WATFEERDKANVVMEPNFDIIIPDGENYTLLGNMLTDSQDHIISAIFGNNEIMQKCKN
                     NRFWLDKMEFTV"
     gene            9896..10147
                     /locus_tag="ACHOQV_003740"
     CDS             9896..10147
                     /locus_tag="ACHOQV_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9632038.1"
                     /translation="MPNIILSDISASVSELKKNPMATVGAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSSQPLHDVDLDSYS"
     gene            10144..10431
                     /locus_tag="ACHOQV_003741"
     CDS             10144..10431
                     /locus_tag="ACHOQV_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9632039.1"
                     /translation="MTYTIKFRDDALKEWLKLDKTIQQQFVKKLKKCSKNPHILSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYYLASERMR"
     gene            10599..11003
                     /locus_tag="ACHOQV_003742"
     CDS             10599..11003
                     /locus_tag="ACHOQV_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFP9632040.1"
                     /translation="MTILVTAQAQLIVGEAMAIKSLAPDGMLAAVFEDDGNTGYFYAL
                     DESVEGNPVRDAVHIYNVEDISDAHIPSDVKIGWSEDCLKCVLLINGYPHGVFDFEGK
                     NGYCRSGFPPPVNHEWSVQGHSWDDYVDNFFR"
     gene            11312..12796
                     /locus_tag="ACHOQV_003743"
     CDS             11312..12796
                     /locus_tag="ACHOQV_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9632041.1"
                     /translation="MRKIIAHFKIVLTLLLPATVSAQSIEWQSCMTTPFSNWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDSSSVKRQQVKLALTKLPAKSRHKGSIIIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSIPAINCQQSDTGIQENITEKQRVLNKI
                     NSCIHNTGTEVIRHIGSNEAVYDIDRIRQALGDEQLTAVAYSYGTQIAALYAERFPSS
                     IRSIVLDGVVDIDDLGDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSVRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELILLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSTPIASEESSDALGVILCVDQGGEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSNLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVRHLITPMKMGNISCPGNAEIDIK"
     gene            complement(13017..>13616)
                     /locus_tag="ACHOQV_003744"
     CDS             complement(13017..>13616)
                     /locus_tag="ACHOQV_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFP9632042.1"
                     /translation="DLEEKKNPVIVVKKRRSFSPPSLAEKKDVIAPALTEQATESVSS
                     GISSPAVETHIPEAPARKKKKKRQRFPRPSHWTSEFTHDCLEKVKALFPQLRAEGGGF
                     LPLKTGITRDITTYLAAHPETALTLDEWNCAVSCITSRRIYLQRTSVAGTPRYNLDGQ
                     PEGEVSECDAQNSRRWLAAKEQRWLSMKARQENISEKSK"
BASE COUNT         3682 a         3087 c         3354 g         3493 t
ORIGIN      
        1 agtgatatcg gagatattga gtccgaatat gacggttaac aacatgataa ataatggttt
       61 ttatgctaag agtgagactt ttggaggaaa tgggcgcaga accccgataa cgaacagggt
      121 gaggaccagc gcaataacgg taaacaacca tcccggaaga ttaaacatag tgagaacctc
      181 tgtatatgtc gggattaaag aacatccggc cagggattca gttcacccag ccagcgggaa
      241 ccggaaagac ggcagacaat aatcactttg tctcctcgtc tgaattcttt cccggcctcc
      301 ggctccagca acaggtaatg aacctgcccg aagcggtctg tgaagcgacc ttcacagggg
      361 gaacccgcca ctgcgtgata accggtgatg gtggccatac agccgatgaa tgcctcctcg
      421 gtgagcgccg ttgtttcatc acggggtaac cagggggcaa tcagtctgcc ggtgcggtgt
      481 acgagaggac atgtcagcag caggcaaagt ggtgcaagga gaatgttcgg ggcaggagcc
      541 tgcagcagat gaataataag accctgcagg agaatgccgg atataccaaa aaaaactgcc
      601 agcatacaga ggacgatcag cagggggatc cgtccgatgt tcagccagtc cagcgcctct
      661 cctgtcagac cctccggtaa gtcaggtgta tccatagctg aagagaggga gtgtccgcag
      721 agcagggcga taagttcagc gataccagta agcagaacaa aagtcagggc tgccagatag
      781 ggtgcgttat acgcggtaag taacgtcagt gtcatttttc ctcctgtggc aggtcagtca
      841 aacaacatta tgcgtgaagc ttccgcttct ttaattgtca gaagaagcat atcgtcagtc
      901 atcagaagac aaggggatgt ctgcaggagg ggatgataac aacagaagcc tcacgccgga
      961 atgtgcttta tgacaacagg agaggacgga taataaaaaa tgggacatcc acatcagcaa
     1021 accgcgattt gcgaaaaaaa gtcagcggag agtggctgcc gttgtggctg tcccggtatt
     1081 tcagtgtaac aggtgattgc tgtgatctat gccccattcg gctttcggta aacctgaatg
     1141 ccccacatga agaatgcgtg acagcatgtt tctgccgctg ccgttcaggc tgcattcgcg
     1201 tccgtgcgcg agtataaaac cttcatcccg gaatatctct tccacttctg aagattcgag
     1261 ggatgccgga tgaattaatg aaaatgccac cggtctgagc attctgcccg tggtggaaaa
     1321 ggtgtctctg gtactgttca gctgcaggat atgacgttta cctgtttgcg cacgggaaag
     1381 tgtctcactc agcagctggt cgttccatgc gcacgtgcca atctgccatc ccagacttct
     1441 gcacgcggaa agagccgagc ggaagcggta caggtcgtat aatgctgcgt tattgccact
     1501 ttccagcagt gatttcaggg tgtttgctac cggtatgtct gaattggtgg ccagcagttt
     1561 tcgcagggta gtgaactctt cagccattcc tctgaactcg cgcatctgac gggcagtgtc
     1621 aagccagttc agcaggtaac gccggcgacg ccgttcgttg tttatgttgt ccggggcggt
     1681 ggagagtgct tcgagcatat tctccagtaa cagaatgaca gggttttcct gggatagaat
     1741 ggtcataaag gcgtcctgaa aaaatgcccg acagccgctc tccgtacacc ctcaggtgtc
     1801 accggtgccg ggcattgtgg aaacagaagg gaaaaaaatc ccggccataa aggccgggaa
     1861 aagtcgtcaa attaagacag caattttcat gtcaacgctc cgtcatcaga taccaggtgc
     1921 aggagcaggg cgatcctcga ctccggcaga ggctgtgcat caagccggag gaggtactgg
     1981 taacgcgctt ttgtcagcca gagggtggag gcagcatcca gctcctccct ggctgccgga
     2041 ttgagcgcag gatccgggta cactttcatc aaagactgaa cgatatcctc cctgacggtt
     2101 ttgatttcac cggccatttt cacgaagttc agaaaggcac tgtgacggcg aaggtgagtg
     2161 aagctatggc gttcggggtc tgctttcagg aaaaagtaac cgggaaacac ggcgtgtata
     2221 tgtcgacggt aaccgcagac tttatccgtg cggcggactt ttttcagtat caggggtgtc
     2281 cagggaagga cgtgttgttc ggaaagccag gaaaagagat gttcacggtt tttgccggca
     2341 gggatgtact gagccagata ccagttgcgt tgatgtaatt cactgatatt caaaatccgg
     2401 aacgctccgc gaaaatcgat ccccgcagag gggcttcgat gttgttgtga acggcgaata
     2461 cataaagcat gtgtcggaaa cagcggctga ccctcgttca gcccggcctg cagttctgac
     2521 aggcacatga tttacgcact cttgtaagcc ttgtggacgg tttcgaggcg ggcgaaaacc
     2581 gacaacttct gcaaacagga ttaatggctg gcaaatatat tacataagga tgtatctgtg
     2641 gcaagagtga agataagcag ttaaatagat cgttatattt aataaatcag catattatgc
     2701 tgatttgtgt gcggtgtctg gaggattaga tcgtttaaaa attttaggcg atctcttcta
     2761 ctaaaaatga tgaaaaacac agcataaaag atcgtttgtg gaattgtcta tttttttgac
     2821 atagttcaaa aaaacgacgt tttttaggtg gttaatgaga ggcctggtaa atcagagaaa
     2881 tcgtgtcggc ttttcaaagc ggtggaaaag gggtatattg cggatcgtta tgcagtggct
     2941 tttgggatcg tcaggatccg ggaagtcaga aaacggcagg atgcgccata aggcattcag
     3001 gatgtatggc agaaacgacg gcagtttgcc ggtgccggaa ggctgaaaaa agtttcagaa
     3061 gaccataaag gaaaaccccc ataatcttct tatcgaactt ggcggaacgt agagaagatt
     3121 atgggggcct cacagaatac gggataagta tatatgaaac cgtaccagag attcaaccct
     3181 tcgcagtgca ttaacgcacg gcattctgtt tccgccataa gcgacagctt gtggcggccc
     3241 tgaaccagac ctcatctact gaaagcacat accgtcaggt aaagaacccg aatccggtct
     3301 tcacaccccg tgaagatgcc gggacgctga agttctgcga aaaactgatg gaaaaggcgg
     3361 tgggtttcac ttcccgtttc gatttcgccg tgtatgtggc gtatgcccgt tcccgggggc
     3421 tgcgtcggcg catgccaccg gtactgcgcc gacgtgccat tgatgcgctg ctgcaggggc
     3481 tgtgtttcca ctatgaccca ctggccaacc gcgtccagtg ttccatcacc acgctggcca
     3541 ttgagtgcgg actggcgacg gagtctgctg ccggaaaact ctccatcacc cgtgccaccc
     3601 gggccctgac gttcctgtca gagctggggc taattaccta ccagacggaa tatgacccgc
     3661 ttattggctg ttatattccg accgacatca cgttcacacc ggcactgttt gccgccctcg
     3721 atatttccga agaggcggtg gcctccgcac gccgcagccg cgtggaatgg gaaaacaggc
     3781 agcgcaaaaa gcaggggctg gatgcgctgg gtatggatga actgatagcg agggcctggc
     3841 gttttgtgcg cgagcgtttc cgtagttatc aggcagagct taagtcccac ggcatgaaac
     3901 gtgcccgtgc gcgccgtgat gcaggcagaa cccggcagga cattgtcacc ctggtgaaac
     3961 ggcagctgac gcgtgaaatc gcggaagggc gtttccgggg aagcctggag gctgtgaagc
     4021 gcgaaatcga ccgtcgcgtg aaggaacgca tgataatgtc gcggaacaac aactacacac
     4081 ggctggccac agcctcaccc tgagttttac ctcatctgaa taatccggcc cgcgccggag
     4141 gcttccgcac gtctgaaggc cggcgacgca caaaaaagca gcgcagcaca caaaaaacag
     4201 cctcatcatc tgccttccgg ctcgccttgc tccccctgtt tttcacgcaa aactcgtctc
     4261 acagacgggg gattctgctt atccacattt aactgcaagg gacttttcca taaggttaca
     4321 accgctcatt ccataaagcg ccagccgcag gcgttacagg gtgcaatgtt tcttttaaac
     4381 cccagtactg tatttccttt aaactactta attagttcca tttaaaaaga aaacctattc
     4441 aatgcctgtc ctgtggacag gcaaatatgt acctcccact ctaacgcgcg ggccccgaac
     4501 ctcaccgttc tgaaaccaca gcaaaaaaac atcaggaata aaaacactac acaaacgcag
     4561 caccgtgccc acccctcata actgaaaagc gaggccgccc ccgcccaaag ggcgggaaca
     4621 acatcgcttt taattatgaa tgttgtaact aaacaacacc atcgctgtca gtcttctggc
     4681 tggacgtact gaatacacgc tcgtaagcgg ccctgacggc ccgctaacgc ggagatacgc
     4741 cccgacttcg ggtaaaccct cgtcgggacc actccgaccg cgcacagaag ctctctcatg
     4801 gctgaaagcg gatatggctt agcagggtgg gaatgggata ggcgaaatct atcaatcagt
     4861 accggcttac gccgggcttc ggcggtttta ctccggtatc atatgaaaca atggagtgcc
     4921 gccttccatg ccgctggcgc ggcatatcct ggtgacgata tctgaatcgt tatatactgt
     4981 gtatatacgt agtaatgacg aggtgataaa tggcacaggt taatatgagt ttaagaatcg
     5041 acgctgaact gaaggatgct tttatggccg ctgcaaaaag catggaccgt aatggctctc
     5101 agttaatccg ggattttatg cgccagaccg ttgaacggca gcataatacc tggttccgcg
     5161 accaggttgc ggcaggacgt cagcaactcg agcacggcga tgtgcttccc catgacatgg
     5221 tcgaatcctc tgctgcagca tggcgggata aaatgagcag gaaggtcgcc ggtaaatgat
     5281 ggagatattc tggactatgc tggccagcca ggacaggaag cgcattcgtg agtacattgc
     5341 ggagcagaac ctgatggccg ccattgaact ggacgaacgg attggttatt cagccagcag
     5401 cctggccggg cagccgtata aaggccgcaa cggtcgtgtg gaaggtaccc gggaactggt
     5461 tattcacccg cattttgttc tggtttatga ggtcgacagc cagtggggaa aagtgtatat
     5521 cctgcgtgta ttgcatactg cacagaagtg gccatagaga ggctgtccgg atataaaaac
     5581 gtctgacgga gatgcaagaa ccatgaataa tgatgagctg gccaccagac gtgcgcaggc
     5641 gattgctgaa gacagatgtt tctcgaaagg acgtctgcgc gatgaattca gaatgaagcc
     5701 tgcccccggt gctgagccgg ttaagtggta taaaaacact tatggtggca ggttcgctgt
     5761 ataccgaatt gcagattgtg ttccgatgcg tgagaaacgc ccactgacga gtaaacaact
     5821 tctggctggc cagcgacttt ccgttttatc cagacttaac agcaccagtg gccggatggc
     5881 acggcaggca tatgactggc tgagtctggc accgctcttt ctggatactg aaacgacagg
     5941 actggataat accgcggaag ccctggaaat cggccttact gatgcggccg gtcaggtggt
     6001 atttgaaacc agactgaagc ccacggtcgc catcggggcg caggccgccg ctgtgcatgg
     6061 tatcagtgag caggctctct gcggcgcacc atcatggaca gatgtggcgc ggcaactgcg
     6121 tcatgctatc gggggccgac cagtaattat ctttaatgcc cggttcgaca tccgcattct
     6181 gaaacagact gctgccgcac atagcgatcc ggcttactgg ctggaagaaa tgacggtata
     6241 ttgtgcgatg gagctggcag caggatatta tggggccacc aaccgctatg gcacgatttc
     6301 actggccagt gctgccagcc aggccggact gacctgggaa gggctggcac actcagcgat
     6361 cgccgacgca cggatgacgg caggggtggt aaacgctatt gctgcatatc atctggaact
     6421 gctgcaggaa caggcacggc tggaaatctg accgcctggc ctgtataccg caatcattta
     6481 cgttatccgg acgtaaatct gccgcttgtt gctgtcagca tgttaatgct aatgtatgcg
     6541 tacattagca ttaatgaggg atgatatgtg agagacaaaa ccagggaggc catgcggctt
     6601 tttttaggcg gcaggtgcta tacggctgaa aaacttgaaa aggattatct ggctgaagtg
     6661 gctaattaca gtaacgaccg ctgggaagca ccgcaacgtg cctcacgcct ggctgccagc
     6721 gtaaagcgtt acaagacatc agaaatgctg cgctttattt ttgctaccat tgcctacgat
     6781 cccgatccag atttaacccc gctgactgtg agacgtttgt gcaaggcatt gttcggcaga
     6841 accggaagcc agtggctggt tgttgaagtt tttggggaga aggggcgtca gcaccgcagc
     6901 gcggacagca atccggagat ggtggaaaaa atggctgcca gatatcgtca tgccgccgaa
     6961 ctacactggt ccgcaacgct ggcagaaatt gaacgggtaa agcggcttta tcagacgaaa
     7021 attaagaaat ccaaaaaaga agtgggttga ctgccattgt tattcttctc cttcatcatt
     7081 aatgctaatg tatgcataca ttagcattaa tgatggtgcg ttgaacgtac aataaggggt
     7141 ccgcttggaa ttcggaaatt ttcccacgct aagagaattg agttgaacga gtggttacat
     7201 ccaggccagc taactcactg aacgtaaaga ctgtacgaaa tgttccgacc gtccgataac
     7261 catttggctt acttccgtac atttccttgc gtagatgtac agaattgtac gttttcgtac
     7321 ggttggccct caaaccccat ccttgagtag tggcataaga aaacggctcc ctgttgtgga
     7381 agccgttatc agttgcacga ttagcattgg agtttaaagg atacctttct gagaaaatgt
     7441 gatccgggtt gcttcattta aatccatata ttttagggtg tcaggagatg cccagactat
     7501 atcctgaaac tcctcattaa atgtaactgt acggtttgta ctgatacagt cgaatattag
     7561 gtaaatcatg taaatgtcct cgtgggtacc atccgggtac gtttttgtac ggatatcatc
     7621 acgaaaggcc cagggtttta cgtcagtgat atgcagttct tttcccagtt cttcctttat
     7681 ttcacgcctc aatgctgttt ccatagtctc tccgggttcc attcctccac cgggcagtgc
     7741 ccattggccc gggaatactc cgcgatcaga ggccatttta caaagtagaa actcaccttc
     7801 attttgtatc acagggcaca ctattactct ctggcgcatt ttatgtcctc aacatcatgg
     7861 ctttttggat aaccttatca gaaaaatcat caatgtcatc cgggtgagca cataccataa
     7921 tccgctcacg aaggtgtaac ggaagattat cagtaaaatg ttgatattcc gcatgccatc
     7981 ctgtgatcca ttttatcttc atttcctcat ctggaaattc ttcaggccgt acaccatgaa
     8041 tttcatcatc attgagatgc atgagtgaat gtgcgatggg ataattaatg aatgctataa
     8101 tgtctggctc tgtaagtaat tcatttattc tactccagag caccatgtat gcatcatatt
     8161 gtgctttatt tatatattcg gacttcagca tgaagttgat aaaaatataa tcctcaatat
     8221 gggatctctc cataataagg ttaaaccctt tatccagcca agattttatc agtaatgctc
     8281 gttgtatcat aaaattaagc tggattagat aaccgtagtt actggtatcg tcaaaaaggt
     8341 aaggaaggag agtgtggtgg acactttttt catctatcgc gacagtgtct ggatctacct
     8401 taaataactc ctttgacatg cgtctcagaa gagtactttt acctacaccg gaattacccg
     8461 acagacatat atacagagga tttcgagttt tattcttctc ttttttatct gctgaccaag
     8521 gatccatata caagtaatta tatctcatac tgtaaactcc attttgtcca accaaaagcg
     8581 gttattctta catttttgca tgatttcatt atttccgaaa attgcactga ttatatgatc
     8641 ttgtgaatct gtcagcatat ttcccaacag agtatagttt tctccatcag ggataataat
     8701 gtcaaaattg ggttccataa caacattggc tttgtcccgt tcttcgaaag ttgcccaact
     8761 aatgggcatg ttagggtgtt gtttttttag cattaccatt ttatctttaa atatatccgt
     8821 attgatttcc agtttatctc ttttttcttt tccgactcct agaggacaga actggtaaaa
     8881 tcgccaagct tggatcttat agggcagaga atcaacccag caaacaatat cactgagatg
     8941 attaatgtta gactgagata cgacggtcgt gagttttata ttaattcgat tattattttg
     9001 gggtaaccat gttaatatat ttttgataac cctgtgttgg ttactcattt tatcactgcg
     9061 aatttctgaa ttgacaagac tgtcaattcc atcaattggt attcctaccc agtctaacac
     9121 cggatttaac cgagcatgat ccataataaa tcgatgcccg accgtggaga gatatgtcat
     9181 aaaacccagt tgcttagcgt attcaatcag atcaatgaaa tcactccggg atgtaggttc
     9241 ccctccggta aaaccgatac gacggaatcc cattttatac atccgctcaa ttacattacg
     9301 agcactagca agagaagagt cctgaattcc aggtgtacga aaacacagat cacatcctag
     9361 gttacaatgt gaagttattc tgaaatctaa aagaagggtg ctaatatcca taacaagtcc
     9421 tctgactatg atacatcaat aaccattaaa acataatggt tattgcagac catgacaaag
     9481 cgcaaccttc ggcttaccag ggatataata tctgccagta atcagagcag tgggttgcgc
     9541 aaggaactca tgaatgatga gttccggtaa tcgcattact ggaagcccat gatcggaggg
     9601 acgctatcag tcacaccagt gaaaattgtg cacagaagca ttttcatcac tgacaggccg
     9661 tatgcctcac aagacgggag ataactggag aatatttagt tcttctaccg gcttacgccg
     9721 attttactcc tgtgttatat gtaaccattg tgtgccacct tccatgcgct ggtgcggcat
     9781 cagagtaaaa aatcagcatt aaatttattt gcaaatgtat gagtaataca gacttgttaa
     9841 cgggtccttt cagatgtatc tttaagactt attaaacagt cttaaaaggt gcattatgcc
     9901 taacatcatt ctgtctgaca tcagtgcaag tgtcagtgaa ctgaaaaaaa atccgatggc
     9961 gacagtcggt gctggtgatg gttatccggt cgccattctg aatcgcaatc agccggcatt
    10021 ctactgtgtt cctgctgaac tgtatgaaag gatgcttgat gctctggatg accaggagct
    10081 ggtgaaactg gttactgaac gtagcagtca acctctgcat gatgttgacc tggacagtta
    10141 ttcatgactt atacgataaa attcagggat gatgcgctta aagaatggct gaaactggat
    10201 aagaccattc aacaacagtt tgtgaaaaaa ctaaaaaaat gtagcaaaaa tcctcatata
    10261 ctgtctgcaa aactacgtgg gctaaaggac tgctacaaaa ttaaacttcg tgcatcggga
    10321 tttcgtctgg tttatcaggt gattgatgat atgttaatta ttgcggttgt tgcggtaggt
    10381 aaacgagaac gtagtaacgt ttattatctc gccagtgaga gaatgagata gtaacataaa
    10441 tagcctttcc cgcagataac tctcagaatt gttattctct ctgtggcctc gcctgaggcg
    10501 gaaaaagaaa agttttgttg agagaatgat tttacctgcc gggatgagtc tggggttatt
    10561 tatattacca gacaaattga aacggaggga catgtttgat gacaatactt gtgacggcac
    10621 aggctcaact catagttggt gaggctatgg cgataaaaag tttagctcct gatggaatgt
    10681 tggccgctgt atttgaagac gacgggaata ccggatattt ctatgcactt gatgagtccg
    10741 ttgaaggtaa ccctgtcagg gatgctgtgc atatatataa cgttgaagac atttcagatg
    10801 ctcacatccc ctctgatgtg aagattggct ggtctgagga ttgcctgaag tgtgttctgc
    10861 tgataaacgg atatccacat ggggtattcg attttgaggg gaagaacggc tactgtcgaa
    10921 gtggattccc gccaccggtt aatcatgaat ggtctgttca ggggcattca tgggatgatt
    10981 acgtcgataa tttcttccgg tgattgaacc gaaaaattac ctgacggaca agtctggggc
    11041 cgaatcgtga tgattcatca tggccgttta tagttcagca gtcttgttct tttgctgtca
    11101 gtgggatacc aggaaaataa ccgattgtta ccagacagaa taaccatcat aagacgtcac
    11161 aagacggtga cgcttacata gttccgggga gttaaacaat ataccctgac tttgtaatca
    11221 tttgaattat atccgtaacg tgtaacttcc tgccgtaaca ccggatttaa gatattcaaa
    11281 ctttatcttt ataccgatat taagagacac aatgcgaaaa ataattgctc atttcaagat
    11341 agttttaacc ttacttctgc ccgcaacagt atctgcccag tcgatagagt ggcaatcctg
    11401 tatgaccact ccattcagta actggtttgg tgaggaaaaa ccgtctcctg acttactatg
    11461 tggttatttg tctgttccat taaaatatac agacacaggc ggagatagtt cttctgtaaa
    11521 aagacagcag gtaaaactgg cgctgacaaa attgccagca aaaagcaggc ataaagggag
    11581 tatcattata ataagtggtg gaccaggatt acctggcatt aatccttata ttaactttga
    11641 ctggccggta acaaatcttc gcgaatcatg ggatattatt ggctttgatc cacggggagt
    11701 cggccagtcc attcctgcaa taaactgcca gcaatcagat acagggattc aggaaaacat
    11761 aactgaaaag caacgggtat taaataaaat taacagctgt atccataata ccggaactga
    11821 agttattcgc catattggtt ctaacgaggc ggtatatgat attgatcgta tcaggcaagc
    11881 cctgggtgac gagcaactga cagctgtggc atattcgtat ggaacacaaa ttgcagcctt
    11941 atatgcagaa cgtttcccat ccagcatcag atctatcgtt cttgatgggg tcgtcgatat
    12001 tgatgacctg ggggacaact tcacatggca actcaaacaa gcacagagtt atcaggaaac
    12061 gttcgatcgc tttgcatcct ggtgtgcacg tacaaaaagt tgcccacttt cttcagtcag
    12121 agataaggca ataactcagt tccatgagct gttatcaaaa ttgcatcaca agcctttatt
    12181 agacagcaag ggagaaaata tatcttcaga tgaactcata ttattaacaa cagacctttt
    12241 gttatggcgt tcatcatggc caacccttgc aactgccata cgtcagttct ctcaggggat
    12301 tgtcagtaat gaaattgaaa ctgcgctcag tactcctata gcctcagaag agtcaagtga
    12361 tgctctgggg gtaatccttt gtgttgatca ggggggggag caattaaccc cagaagagcg
    12421 aaaatcccga aaagatgctc ttgcgaatgc cttcccggct attaactttg acaatggacg
    12481 ttccgattca cctgattttt gtgaattatg gccaatacat agcaacctga ataaaacccg
    12541 gctgaaaaat acagttctgc cctctggttt actgtttgta gcacacaaat acgatccaac
    12601 aacaccatgg attaatgctc gtaagatggc agagaaattt tccagcccgt tgctgacaat
    12661 aaatggtgat gggcatacat tagctcttac cggagtcaat ttatgtgtag ataaggccgt
    12721 tgttcgtcat cttatcaccc ctatgaaaat gggaaatata tcctgtccag gaaatgctga
    12781 aatagatata aaatagttta aaaattgccc gccatttgct cctgataccg gacatatttt
    12841 gcattgggag caaaatgcgt tcagtgcgat cacggcacca gaaacgtcaa actggataca
    12901 cgtaaaaaat gtcgcgatcg cgcaaaatca agccattact ctcgggagtg cttaaataat
    12961 acgcacaata tacaacgctc tcttctctac agtctgtttc ctgtaatatc ctgcggttat
    13021 tttgatttct cagaaatatt ttcctgccgg gccttcatac tcagccacct ctgctctttc
    13081 gccgccagcc agcgcctgga attttgagca tcacattccg atacttcccc ctctggttga
    13141 ccatccaggt tataacgggg agttcctgca acagaggttc tctgcaggta gatccggcga
    13201 ctggtaatgc aggatactgc acaattccat tcgtccaggg tcagcgctgt ttccggatgt
    13261 gctgccagat atgtagtaat gtcacgggtt atgcctgttt tcagaggcag aaaaccgccc
    13321 ccttcggcac gaagttgagg aaacagcgct ttcaccttct ccagacaatc gtgagtgaac
    13381 tcactggtcc agtgggatgg tcttggaaag cgttgtcttt tctttttctt cttcctggct
    13441 ggcgcttcgg ggatatgagt ttcgacggca ggtgaactga ttccggagga gactgattct
    13501 gtcgcctgtt cagtgagtgc aggcgcgata acatcttttt tttcggctaa ggaaggcggt
    13561 gaaaaagagc gacgtttttt caccacaatg acgggatttt tcttttcttc cagatc
//
LOCUS       DAZKGF010000135        13443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZKGF010000135 DAZKGF010000000
VERSION     DAZKGF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.135"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            196..909
                     /gene="ydiV"
                     /locus_tag="ACHOQV_003745"
     CDS             196..909
                     /gene="ydiV"
                     /locus_tag="ACHOQV_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFP9632043.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLDEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEIMAQITPFDFHAL
                     QGCLWPAVPVNQITTLVQR"
     gene            972..2408
                     /gene="selO"
                     /locus_tag="ACHOQV_003746"
     CDS             972..2408
                     /gene="selO"
                     /locus_tag="ACHOQV_003746"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFP9632044.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQRLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVALSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYASRPPDWGKRLEVSCSS"
     gene            complement(2412..2603)
                     /gene="hemP"
                     /locus_tag="ACHOQV_003747"
     CDS             complement(2412..2603)
                     /gene="hemP"
                     /locus_tag="ACHOQV_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFP9632045.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2735..3781)
                     /gene="aroH"
                     /locus_tag="ACHOQV_003748"
     CDS             complement(2735..3781)
                     /gene="aroH"
                     /locus_tag="ACHOQV_003748"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFP9632046.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3938..4771)
                     /gene="ppsR"
                     /locus_tag="ACHOQV_003749"
     CDS             complement(3938..4771)
                     /gene="ppsR"
                     /locus_tag="ACHOQV_003749"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFP9632047.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            5104..7482
                     /gene="ppsA"
                     /locus_tag="ACHOQV_003750"
     CDS             5104..7482
                     /gene="ppsA"
                     /locus_tag="ACHOQV_003750"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFP9632048.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(7539..9185)
                     /gene="fadK"
                     /locus_tag="ACHOQV_003751"
     CDS             complement(7539..9185)
                     /gene="fadK"
                     /locus_tag="ACHOQV_003751"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFP9632049.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSALSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPAACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFIHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEDVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(9242..9535)
                     /locus_tag="ACHOQV_003752"
     CDS             complement(9242..9535)
                     /locus_tag="ACHOQV_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP9632050.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(9532..10821)
                     /locus_tag="ACHOQV_003753"
     CDS             complement(9532..10821)
                     /locus_tag="ACHOQV_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9632051.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLDAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(10876..11814)
                     /locus_tag="ACHOQV_003754"
     CDS             complement(10876..11814)
                     /locus_tag="ACHOQV_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFP9632052.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQTV
                     MPYGPKCIYVLAQNDALQRTENYAESIAALLKDKHPSMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSN
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(11834..12598)
                     /locus_tag="ACHOQV_003755"
     CDS             complement(11834..12598)
                     /locus_tag="ACHOQV_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFP9632053.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            12914..>13443
                     /locus_tag="ACHOQV_003756"
     CDS             12914..>13443
                     /locus_tag="ACHOQV_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFP9632054.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYI"
BASE COUNT         3257 a         3345 c         3465 g         3376 t
ORIGIN      
        1 tacagtagta ttttgcagac aaaaattccg taaatggatt attcgtttat aatcaggata
       61 atctaaaaaa aggacccctg aaaaagtggt taacatggaa caaatgaaag ttactccttc
      121 tgtaattgtt atgtcttatg gcctcatccc ttttagccgg atactgaaaa acatccttcg
      181 agagggacgg ttaccatgaa gatttttttg gagaatcttt atcattctga ttgctatttc
      241 ctgccgataa gagataacca gcaagacctt gtcggcgttg agttaattac ccatttctcc
      301 agtgaagatg gtacggttcg tatcccgacg agtcgggtta tagcgcaact taccgaggag
      361 caacattggc aattattttc agagcagttg gaattactaa aatcatgcca acactttttt
      421 attcaacata aactctttgc ctggcttaat ttaacgccac aagtggctac attattatta
      481 gacagggata attacgcagg cgaactatta aaatatccgt ttattgaact gttgattaat
      541 gagaattacc cgcatctcga tgaaggaaaa gataatcgcg atcttttgtc cttatcgcaa
      601 atgtatcccc ttgtgttagg taatttgggc gcaggcaata gcacaatgaa agcggttttt
      661 gatggtcttt tcacccgcgt catgctggat aaaagtttta ttcagcaaca aatcacgcat
      721 cgctccttcg aaccgtttat tcgtgcgatc caggctcaaa tttccccatg ttgcaattgc
      781 atcatcgcgg ggggcattga tacgccagaa ataatggcac agattacccc ctttgatttc
      841 catgctctcc agggatgcct gtggcctgcc gttcctgtaa atcagataac gacgttggtt
      901 cagcgataac cctcctgttt gctggtgttt aagacgagag taaccgtcta cactatcaaa
      961 taggaggatc tatgaccctg tcttttgtta cccgctggcg cgatgaattg ccagaaacct
     1021 atacagcact ttcccctacg cctttaaata atgcccggct gatttggcat aataccgaac
     1081 tcgctaacac gctgagtatt ccatcgtcgc tgtttaaaaa tggcgcaggt gtctggggcg
     1141 gcgaaaccct actgcctggt atgtcaccac tggcgcaggt ttacagtggt catcagttcg
     1201 gcgtctgggc gggccaactg ggtgatgggc gcggcatttt actcggcgaa caacggcttg
     1261 ctgatggcac tacaatggac tggcatctga aaggtgctgg cctgacgcct tattcgcgaa
     1321 tgggtgatgg acgggcggtt ttacgttcga cgatacgaga aagtctcgcc agtgaggcga
     1381 tgcattatct gggcattccg acgacccgcg cgttaagtat cgtcaccagc gattcgccag
     1441 tgtatcggga aacggtggag ccaggcgcga tgctgatgcg tgtggcacta agtcatctgc
     1501 gctttggtca tttcgaacat ttttactatc gccgcgagcc ggaaaaggtt cgtcagttgg
     1561 ctgactttgc cattcgtcat tactggtctc atcttgaaga tgatgaggac aaataccgtc
     1621 tctggtttag cgatgttgtc gcacgtaccg catcgttaat tgcccaatgg cagacggtcg
     1681 gctttgctca tggggtgatg aataccgaca acatgtcgct gctggggctg acgcttgatt
     1741 acgggccgtt tggttttctt gatgattacg aacccggttt tatttgtaat cactcggatc
     1801 accaagggcg ttacagcttt gataatcaac ctgccgtcgc gttgtggaat ttacagcgtc
     1861 tggcgcagac attgtcgcca tttgtcgccg ttgatgccct gaatgaggcc ctggacagct
     1921 atcagcaggt tttgttgacg cattatggac aacggatgcg gcagaaactg ggcttcatga
     1981 cggagcaaaa agaggataac gcgctactaa atgaattatt cagtctgatg gcgcgagagc
     2041 gcagcgatta tacccgcaca tttcgcatgc tgagtctgac cgagcagcac agcgcggcgt
     2101 caccgctacg tgatgagttt attgatcgtg cggcatttga tgactggttt gcccgttatc
     2161 ggaggcgttt gcaacaagac gaggttagcg atattgagcg tcagcaactg atgcaaagcg
     2221 ttaacccagc tctggtgttg cgcaactggt tggcgcaacg ggcgattgaa gcggcagaaa
     2281 agggtgatat gacggaattg caccgcctgc atgaggcgtt gcgaaatcct ttcagcgaca
     2341 gagatgatga ctacgccagt cgtccacctg actggggtaa acggctggaa gtcagctgct
     2401 cgagttaaag gttacttggt caacagcagc ttgccagcct gagttttacg gagcagatat
     2461 tcttgcccgt catgatcgat aatcagtttg ccatccggac ctaacagtgt ctggctggta
     2521 attcgccgga tgggctgcgg ggaagcggtc ttgtgattgg catccgtttc aggcgtgagt
     2581 tttctgctat ccgtataacg cataaaaatt aataataatt tatataacaa tgattcttat
     2641 tatcaatatt atggcgggcc agagcaagca gtaatttgtt gagaagacaa caagttgcgc
     2701 agaaatgccc atcccgtcag gaatgggcac gcattcagaa gcgagtatct accgcagagg
     2761 cgagtttttc gaccaggcgt tcggtatctt cccagcccag acacgggtcg gtaatggatt
     2821 gaccgtaagt gagcggctga ccgccgacga ttttttgcgt tccttcgcgc aggaaacttt
     2881 ccgccataat tccagcaatc gccgtagatc cattgcggat ttgctgacaa atatcctcac
     2941 aaacttctaa ctggcgtcgg tgctgcttct ggcagttacc gtggctgaaa tccaccacca
     3001 gatgttcagg taaatcaaac tcgtgcagcg tatcgcaggc tgcggcgata tcatcggcat
     3061 gataattcgg ttttttgccg ccacgcataa taatgtggcc atacgggttg ccgctggtct
     3121 gatagatggt catctgacca tttttgtctg gcgagaggaa catatggctg gcgcgggctg
     3181 cgcggatagc atccacggca atccgcgtgt tgccatcggt accattttta aaacctaccg
     3241 gacaggagag tgccgaagcc atttcacggt ggatctgact ttcggtagta cgtgcgccaa
     3301 tcgcgcccca actgattaaa tcagcaataa actgaccggt caccatatcg aggaactcag
     3361 tcgcggtagg gacgcccagc tcatttacct gtaaaagtaa tttgcgcgcc agctccagac
     3421 cgtgatttac ccgatagctg ccgtttaaat ctggatcgga gattagccct ttccagccga
     3481 caacagttcg tggtttttca aaataggtgc gcattacgat ttccagccgt gactggtact
     3541 ggttgcgcag cgactgcaga cgggtggcgt actccattgc agcggtgaga tcgtggatcg
     3601 agcaggggcc aataatgacc aacagtcgct tatcttcacc attcagtatt ttttcaattc
     3661 tgcggcggga gtcggtgaca tgggtggcga cgccaggcgt tacgggatac cgtagcgcga
     3721 gttcggcggg cgttaccagg ctctcaatac gcgcagtacg gagttcgtca gttctgttca
     3781 ttacaagtct cagaaatttg ttgcttctac ggcaggacta ccgggaagtg atgcgcatca
     3841 cgataaacca atcgcagctg atttcaagtg cgaggtgtgt cacacctcgc cgggtggtta
     3901 gcatgataac aaaaaaacaa gctaatgcac tagttctcta gtacattcga cggctaaggc
     3961 ccatgatatc gaggatcttg gtggcaatct cttctaccga ataattggta ctgttaatcc
     4021 acgggatctg atttttacgg tacaacgctt ccacttccgc gacttccatc ctgcactgac
     4081 gaagcgaggc atagcgactg ttctcccgac gttcctcgcg aatcgccgcc agacgttccg
     4141 ggtcgatagt caggccgaac aatttatgct gaagcggttt gagcgacgcg ggtagcacaa
     4201 gattatccat atcgtcggca ataaaggggt agtttgccgc gcggatacca aactgcattg
     4261 ccagatacag actggtgggg gttttaccac agcgagaaac gccgagcagg atcacctgag
     4321 cctggtccag attgcgtaac gaaatgccat catcgtgggc gagggtgtaa tcaatcgcag
     4381 caatgcgcgc atcatattta ttgagattat tagggttaag gccatgggta cgatgagcaa
     4441 tcggcgttgg atccagtttc atctcttgtt gtagcggggc aaccagcgcc tgaacgatat
     4501 cctggcaaaa gccctcactt tgcaagatga tggcgcgaat ctccggcaac acgatggagt
     4561 agaagaccag cgggcgcaca cctgtctggt gataaattgc gtcaatctga tccttcactg
     4621 cccgtgcacg gctctcattt tcgacaaacg gcagcgtaat gctgctgata gtgacgggaa
     4681 attgtgacat tactgcgtgt cctaatacct ccgcagttat tgccgtacca tcagaaatat
     4741 aaaaaacgtg gcgatcaaca gcattatcca ttttgttctt cccgtgatgc agacataatt
     4801 ctatgaaagc ataaattaaa aacgcacaga agcgtagaac gttatgtctg gtttataaaa
     4861 tgaaccttca attttatttt ttatgaaaac agcatttcat ttttacggtt tcgtttatac
     4921 cgatggttta tgtggaaatt gtcgaagaga gcagatttgc gcaacgctgg gatcagtctt
     4981 aaaaagtaaa aaaatatatt tgcttgaacg attcaccgtt tttttcatcc ggttaaatat
     5041 gcaaagataa atgcgcagaa atgtgtttct caaaccgttc atttatcaca aaaggattgt
     5101 tcgatgtcca acaatggctc gtcaccgctg gtgctttggt ataaccaact cggcatgaat
     5161 gatgtagaca gggttggggg caaaaatgcc tccctgggtg aaatgattac taacctttcc
     5221 ggaatgggtg tttccgttcc gaatggtttc gccacaaccg ccgacgcgtt taaccagttt
     5281 ctggaccaaa gcggcgtaaa ccagcgcatt tatgaactgc tggataaaac ggatattgac
     5341 gatgttaccc agcttgcgaa agcgggcgcg caaatccgcc agtggattat cgacactccc
     5401 ttccagcctg agctggaaaa cgccatccgc gaagcctatg cacagctttc cgccgatgac
     5461 gaaaacgcct cgtttgcggt gcgttcctcc gctactgcag aagatatgcc ggacgcttct
     5521 tttgccggtc agcaggaaac tttcctcaac gttcagggtt ttgacgccgt tctcgtggct
     5581 gtgaaacatg tatttgcttc tctgtttaac gatcgcgcca tctcttatcg tgtgcaccag
     5641 ggttacgatc accgtggcgt ggcgctgtcc gccggtgttc agcggatggt gcgctccgac
     5701 ctcgcatctt ctggcgtgat gttctccatt gataccgaat ccggctttga ccaggtggtg
     5761 tttatcactt ccgcatgggg ccttggtgaa atggtcgtgc agggtgcggt taacccggat
     5821 gagttttacg tgcacaaacc gacactggcg gcgaatcgcc cggctatcgt gcgccgcacc
     5881 atggggtcga aaaaaatccg catggtttac gcgccgaccc aggagcacgg caagcaggtt
     5941 aaaatcgagg acgtaccgca agaacagcgt gacatcttct cgctgaccaa cgaagaagtg
     6001 caggaactgg caaaacaggc cgtacaaatt gagaaacact acggtcgccc aatggatatt
     6061 gagtgggcga aagatggcca caccggtaaa ctgttcattg tgcaggcgcg tccggaaacc
     6121 gtgcgctcac gcggtcaggt catggagcgt tatacgctgc attcacaggg taagattatc
     6181 gccgaaggcc gtgctatcgg tcatcgcatc ggtgcgggtc cggtgaaagt catccatgac
     6241 atcagcgaaa tgaaccgcat cgaacctgga gacgtgctgg tcactgacat gaccgacccg
     6301 gactgggaac cgatcatgaa gaaagcatct gccatcgtca ccaaccgtgg cggtcgtacc
     6361 tgtcacgcgg cgatcatcgc tcgtgaactc ggcattccgg cggtagtggg ctgtggtgat
     6421 gcaacagaac ggatgaaaga cggtgagaac gtcactgttt cttgtgccga aggtgatacc
     6481 ggttacgtct atgcggagtt gctggaattt agcgtgaaaa gctccagcgt agaaacgatg
     6541 cctgatctgc cgttgaaggt gatgatgaac gtcggtaacc cggaccgtgc tttcgacttc
     6601 gcctgcctgc cgaacgaagg cgtcggcctt gcgcgtctgg aatttatcat caaccgtatg
     6661 attggcgtcc acccacgcgc actgcttgag tttgacgatc aggaaccgca gttgcaaaac
     6721 gaaatccgcg agatgatgaa aggttttgat tctccgcgtg aattttacgt tgggcgtctg
     6781 actgaaggga tcgcgacgct gggtgccgcg ttttatccga agcgcgtcat tgtccgtctc
     6841 tctgatttta aatcgaacga atatgccaac ctggtcggtg gtgagcgtta cgagccagat
     6901 gaagagaacc cgatgctcgg cttccgtggc gcgggccgct atgtttccga cagcttccgc
     6961 gactgcttcg cgctggagtg tgaagcagtg aaacgtgtgc gcaacgacat ggggctgacc
     7021 aacgttgaga tcatgatccc gttcgtgcgt accgtagatc aggcgaaagc ggtggttgag
     7081 gaactggcgc gtcaggggct gaaacgtggt gagaacgggc tgaaaatcat catgatgtgt
     7141 gaaattccgt ccaacgcctt gctggcagag cagttcctcg aatatttcga cggcttctca
     7201 attggctcaa acgacatgac acagctggcg ctcggtctgg accgtgactc cggagtggtg
     7261 tctgaactgt tcgatgagcg caacgatgcg gtgaaagcac tgctgtcgat ggcgattcgt
     7321 gccgcgaaga aacagggcaa atatgtcggg atttgcggtc agggtccgtc cgaccacgaa
     7381 gactttgccg catggttgat ggaagagggg atcgatagcc tgtctctgaa cccggatacc
     7441 gtggtgcaaa cctggttaag cctggctgaa ctgaagaaat aaaataaatc cccggcggcg
     7501 ttttgtcgcc gggtttatgt gatccccgaa gatgaaactt attcaatctc ttcacagaca
     7561 tcctgagtta atcgccgcat aatatctttc cttaacaaaa acttttgtat tttacctgag
     7621 gctgtgcgcg gtagtttttc gattaccacg atatgttcag gatatttata tttcgcgacc
     7681 cgtttacggc taaaaaatgc cactacatct tccagcgata atgaatgatg cggtgctttc
     7741 agcacgacat aagcgcatga acgttcacct aagcgttcat cgggcattgc aaccacacaa
     7801 gcatcgtgaa ttttaggatg ctgcaataaa atatcttcta cttcacggct gctaatattt
     7861 tcgccgccgc ggacaataat atctttcttg cgcccggtta tttttatata gccagcctca
     7921 tccatgcggc agagatcgcc gctgtaatac cagccttctt catccagcgc acgggcggtt
     7981 aattcaggtt catcaaaata ccccataaac acattggggc cacgcgaggc ttcttcacct
     8041 tcgcaacctg gcggtaaagt cttgcgtgcg tcatcgacca ctttaatctc tacacctgcg
     8101 gcagcgtaac catcggtgtg tataaagcgc gacaaaggat catcgagatt caccaccgca
     8161 tgcggcgaac tttctgtgga accataaaca cttaataatt taatgccgcg ctgctggcat
     8221 tcacgcgcca cttttttggg gattgtggta ccgccgcaaa gaaagaaacg cagcgctgaa
     8281 aggtctgcgg gctgtttctc cagtaaattc aaaagatcat agacaaacgg cgttgcgccg
     8341 agcatacagg tgcaacgctg ctgctcaagc agcgcgagac acgcagcagg agtgaaaata
     8401 tctaacaaca cgctgcgagc accaatcaaa aaaggtgctg ttacgccatg cagaaagccc
     8461 gttgcgtgac caagtggcgc aggcatcata aagacatcct gccaggtcag atttagccgc
     8521 gcgcaataag cccgctcact ggcgagaata ttgttatgcg ttagcatcac gccctttggc
     8581 agaccctcgg ttccggaggt aaacagcacc gcggctaatt catcgccgtg gaccgttatc
     8641 gccgtggtca gaggagtatt gtcggcgata atctgactta atgagagagc agaggtggcg
     8701 ggagccagtt tgtccacgcc gacaatttgt tgtagttgtg gaagctgatt ttgcagcggc
     8761 aggattaaat ctaccggacg cgtttgttta aacaacgtcg gtgcaaagaa catttttgcc
     8821 tgacacttat tgagcaccca caccagttct gcttctcgcc aggaaggcaa cagcggcacg
     8881 gaaaccgcac cggttttcag gcaggcaaga tagataacgg taaattcaca ccagcctggc
     8941 agttgaaatg cgatgcgatc gcctgattca atacccttcg ctaacatcca gtttgccaga
     9001 cagctcgcgg cgtgatcgag cgcgctataa gtgtacgttg caccatgatt gtcgaccacg
     9061 gcaattttgt ctggcatcgc acgagcggtc tgctgccagt aatcggccag tgaagcatcg
     9121 ccccataacc cttgctgacg atacgccgca cgacgttgtt cgttgaacgt taatgtcact
     9181 ttcattttgg actctcgaaa cagaacatca ggcccgagat gcgggcctgt gggatgcatg
     9241 attagccgta gcgaaattca ataccgaagg tgcctgcggg atattgccac tgttcgagaa
     9301 tagtgttgcc gcacagcacc cgacaggtgc cgcactccag acaaccggcg gaatcgaaat
     9361 gaatgtttcc tgcgtcatcc tgcttataaa gtccggcagg gcaggctttc attaatttac
     9421 ggaattcatt gatatcggga tttgccgcca gaatgatatg cgggtggccc tcatcaacat
     9481 ggaatttatt tacgcctaat ttgatgtcga cgttaaccgt agcgttctgg ctcatagcgc
     9541 ggttgctccc ttaatgccat ctttcagcaa gttgatcagt ccaattttct tcgcgtgtcc
     9601 catgatcatt ttgcgtaccg gttggttagg tttgccgtcg atggtgaaca tctcgttcat
     9661 gatgtcggcg accattcgtg ggtattggct aaacaggcgc gggttttcca tcagcgccgg
     9721 gatcttgcga aaatgctgca tatcacgcat gacgcagctt tgttccagct cacgtttgta
     9781 ttgcgcaaga ctgctggcgg agaaatccgc gcgttctttg gcggcgatca ctgttgtggc
     9841 ggcagcctga gccgatgcaa tggctaaatc catgccgcgg accgtaaaac ccaaattcag
     9901 gcagaagcct gcggcgtcac caacgatcat cacgccatcg ttaaccagct gcggcaccat
     9961 tgccagaccg ccctccggca ccatatgcgc ggagtattca agcagtttgc cgccgctaat
    10021 cagcgggcga atggcggggt gttgtttaaa atcttccagc atttgcggca cacttttttg
    10081 cgcatgggcg atatcaccca atccacaaac cagccccaac gatacggaat ctttgttggt
    10141 atagaggaat ccgccgccca tcaggccgtc agaaggggaa ccggcaaaca gccaggcggc
    10201 accttcctcg cccgtaacat taaagcgatc gttgatctgt tctggtgtca ggcctataac
    10261 ctctttaaca ccaacagcgt aatgatgcgg atcggaagcg gaaaccattc ccagcgagcg
    10321 gccaagcatc gagttaacgc catcagccag aatcaccaca ttcgcttcga gaatatcatc
    10381 cccggcctgg acgccagtga ccttgtttcc ttcacgaacc agtgcatcga cgcgtacgcc
    10441 cgggataaac tgcgcgcccg cctgctcggc ttgttccatc aaccacgggt ccagacgatt
    10501 acgcaatacg gtataagacg cgtgttgcgg aacatctggt tgctcgcggt gaaaatcgag
    10561 ggtaacggcg ctttcttcgg ttaagaagga gattttctcg cgtgtgacct tgcgttctac
    10621 cggcgctgat gctgcaaagc ctggaataat tgcgtcaagt gtgtgggcat aaagacgccc
    10681 gccggtcatg tttttacatc cggcactgtc gccgcgttct atcaccagga catccagccc
    10741 ggctcgcgcc atgaccagtg cggcaacgct accggcaacg cccgcaccga ccacaatggc
    10801 atcaaatttg tcatccgaca tgcgttatgc tcccttcccg ctgcgtttac agcgggagat
    10861 gtggttgaaa gggaatcagc gactcaactg gctaatcagg gcagggacga ctttgtagat
    10921 atcgcccacc agaccgtagt cggcatagtt gaagattggc gcatttttat ctttattaat
    10981 ggcgacaatc acttttgcgc cgttgccgcc aaccatatgc tggatctgcc cggagatccc
    11041 cagcgtcagg tagagatcgg atttcagcaa cacgccggag acaccgatat aacgttcacg
    11101 ctccatccag ttctcgcctt cggcaattgg acgtgaacag ccgacttcag cattcagcac
    11161 cgccgccagt tcgtggacca ttttcaggtc atcctgcgct gccagaccac gaccgacgcc
    11221 aaccacacgt tttgctttgc tcaggtccac gctgcttgcc gctttcgcac ggcgttcgcg
    11281 acagagaatt tcatgacgcg gggcaacata aggtaccgtt tctgtcggac actgattaga
    11341 ggtatcactg gtgcacggtt cctgaacacc gggcgcaagg gtaatgatcg ccagcgggct
    11401 attgatcttt tcctgtgcga acgctaaccc gccatacatc cggtgttcgg cgcaaatgtg
    11461 accatcgaca atatccaccg cagtggcatc gttcaccagc gccgcattca gttgcacact
    11521 taaccgtgca gccagagctt taccgcgttt cgtcgcggcc agcagcaaca tagaagggtg
    11581 tttatctttc agcagggcag caatgctttc ggcgtaattt tcagtacgtt gcagcgcgtc
    11641 gttttgcgca agaacataaa tgcattttgg cccataaggc ataactgtct gcgcctggtc
    11701 ggtattttgt acaatggcat acacctgttg gccccattgc tgagcgccgc caaacagttc
    11761 agcataacgt tcaggattat cgctaaagac ccagacgctg tttaattgac tcatcgtttc
    11821 ttctccgtag gcttcagttc agggctttct tcagatgttc cgccagctcg gcaatggcct
    11881 ccggcgaatc gttatcgaga atgatgtgct tacgttctgt ttgcggcggt acgcgaatgc
    11941 cggtcagttc agcaagtggt gcgctctggc tccagccaat atcacttgcc tgccactgat
    12001 ttaccggttt tttacccgcg ccgaggatgg ctttcatcga aggaatacgt ggtacgttaa
    12061 tatcggaggt gacgcagagc acggctggaa cagagagttc aataacttca acatcatctt
    12121 caagcgtgcg ttcaatcacc agtgtattgc cctgacgctg aatggcactc actgcattaa
    12181 tcaccggaag ttgcagaatt tctccgacca gcaagccaac ctgctgggca taaaggtcgc
    12241 cggaaccttc accaaagatc agtaaatcga agccgatctt ttcaactgct gtcgccagcg
    12301 cctttgcggt atcgagaggc agtgcatgct caagttgtgc atcctgcacc atatacaggc
    12361 tgtgcggccc gcgggatagc acgtctttgc gcactttcga gttctgcaac aaagagccgc
    12421 cgacggtcag cgcggctatc tcatcgtcat ctgttgcaag ctggcttgca gcttcaatgg
    12481 cattgagatc gaactggctg attttggcgt cggcattgtc gaaattcagg gtatattctg
    12541 gagtgacaac aatgtcctgt tcttcaggca ccagcttaaa gcaggttatt attttcatga
    12601 tttctcctgt gagtcgcata cggaaccatc tccgatggaa ggtaaaaatg gcctgaaaat
    12661 agtgtgcgat agcggtgcgg ggttttcgac ggagcaagct gttttccttg ccaacaatct
    12721 tgcaaaaaaa atgcgatcgc taaaacaaaa gtgcaatttg cggcaatgtt gttattccat
    12781 gaccgtacaa tcttgcattc agtgtaaata atcttgttta gctctcgcta aacgcggttg
    12841 tataaccgca atacacgccg tattttatcc ggcatatatt gtttttgctt gctcatggaa
    12901 tagggaggtt gtcatgtatc aacgctgttt tgataacgcc agcgaaacgc tgtttgtcgc
    12961 cggtaaaacg ccacggcttt cacgttttgc atttagcgat gatccaaaat gggagtccgg
    13021 acatcacgtt catgacaatg aaaccgagtt gatttacgtc aagaaagggg tagcaaggtt
    13081 taccatcgat tcttcgttat atgtcgcgca tgccgatgac attgtggtga tagaacgtgg
    13141 caggttgcat gcggtggcct ctgacgttaa cgatccagca acgacgtgta cctgtgcgct
    13201 gtacggcttt cagtttcagg gggctgagga aaatcagcta ctgcaaccgc attcttgccc
    13261 ggtaattgcc gcagggcagg gaaaagaagt cattaaaacc ttatttaatg agctaagtgt
    13321 gattttgccg caaagtaaaa atagccaaac atcttcgtta tgggacgcat ttgcctatac
    13381 gttagcaatt ctttactatg aaaactttaa aaatgcttat cgttcggagc aggggtatat
    13441 taa
//
LOCUS       DAZKGF010000136        13336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZKGF010000136 DAZKGF010000000
VERSION     DAZKGF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.136"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(92..168)
                     /locus_tag="ACHOQV_003757"
     tRNA            complement(92..168)
                     /locus_tag="ACHOQV_003757"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Met,seq:cat)
     gene            377..1474
                     /gene="mltA"
                     /locus_tag="ACHOQV_003758"
     CDS             377..1474
                     /gene="mltA"
                     /locus_tag="ACHOQV_003758"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFP9632055.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1551..2357
                     /gene="tcdA"
                     /locus_tag="ACHOQV_003759"
     CDS             1551..2357
                     /gene="tcdA"
                     /locus_tag="ACHOQV_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFP9632056.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2408..2851)
                     /gene="csdE"
                     /locus_tag="ACHOQV_003760"
     CDS             complement(2408..2851)
                     /gene="csdE"
                     /locus_tag="ACHOQV_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFP9632057.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2851..4056)
                     /gene="csdA"
                     /locus_tag="ACHOQV_003761"
     CDS             complement(2851..4056)
                     /gene="csdA"
                     /locus_tag="ACHOQV_003761"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFP9632058.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATTLKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPHLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4248..4475
                     /gene="ygdI"
                     /locus_tag="ACHOQV_003762"
     CDS             4248..4475
                     /gene="ygdI"
                     /locus_tag="ACHOQV_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFP9632059.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4826..5743
                     /gene="gcvA"
                     /locus_tag="ACHOQV_003763"
     CDS             4826..5743
                     /gene="gcvA"
                     /locus_tag="ACHOQV_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFP9632060.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5762..6157
                     /gene="ygdD"
                     /locus_tag="ACHOQV_003764"
     CDS             5762..6157
                     /gene="ygdD"
                     /locus_tag="ACHOQV_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFP9632061.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6150..7250
                     /gene="rlmM"
                     /locus_tag="ACHOQV_003765"
     CDS             6150..7250
                     /gene="rlmM"
                     /locus_tag="ACHOQV_003765"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFP9632062.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7294..8025)
                     /gene="fucR"
                     /locus_tag="ACHOQV_003766"
     CDS             complement(7294..8025)
                     /gene="fucR"
                     /locus_tag="ACHOQV_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFP9632063.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVAASLVTA"
     gene            complement(8083..8505)
                     /gene="fucU"
                     /locus_tag="ACHOQV_003767"
     CDS             complement(8083..8505)
                     /gene="fucU"
                     /locus_tag="ACHOQV_003767"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFP9632064.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8507..9925)
                     /gene="fucK"
                     /locus_tag="ACHOQV_003768"
     CDS             complement(8507..9925)
                     /gene="fucK"
                     /locus_tag="ACHOQV_003768"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP9632065.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10034..11809)
                     /gene="fucI"
                     /locus_tag="ACHOQV_003769"
     CDS             complement(10034..11809)
                     /gene="fucI"
                     /locus_tag="ACHOQV_003769"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFP9632066.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQIFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11842..13158)
                     /gene="fucP"
                     /locus_tag="ACHOQV_003770"
     CDS             complement(11842..13158)
                     /gene="fucP"
                     /locus_tag="ACHOQV_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP9632067.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3222 a         3307 c         3515 g         3292 t
ORIGIN      
        1 ctcaggcttc actgaaacgg gtaaggaagc gtaaaaacga aaaaaccgca agccaatagt
       61 agctttgcgg tttgtcagca ttctaataaa ttggttgcgg gggccggatt tgaaccgacg
      121 accttcgggt tatgagcccg acgagctacc aggctgctcc accccgcgtc cgtggatgcg
      181 cactatactc tgctcgcgtt ttcatgcaac ctttttttta ccccgatcac ggattcggca
      241 tgattttgaa caaaaacaaa tcactaaagc ctattttttg tgcaaaattt gcgtcaggga
      301 gaggctgctc atttcattaa acgcggtggt ttgttatctt cgttgcgcct tattttttaa
      361 cctgaagaag agaacaatga aaggacgttg ggtaaagtac cttcttatgg gcacggttgt
      421 ggcaatgctt gccgcctgct cttccaaacc aaccgatcgc ggacagcaat ataaagacgg
      481 gaaatttacc cagcctttct ctctggtgaa ccagccagat gccgttggcg cgccgattaa
      541 tgccggtgat tttgccgagc aaattaacca tatccgtaat tcgtcaccgc gtctgtatgg
      601 caaccagagt aatgtttata acgcggtgca agagtggctg cgcgcaggcg gtgatacccg
      661 caatatgcgc cagttcggca ttgatgcctg gcagatggaa ggtgtcgaca actatggtaa
      721 cgtgcagttt acaggctatt acacgccggt aattcaggcg cgccataccc gtcagggcga
      781 gttccagtat ccgatttatc gtatgccgcc aaaacgtggt cgtctgccgt ctcgtgcgga
      841 gatctacgca ggagcgctga gtgataaata tattctcgct tacagtaact ccctgatgga
      901 taacttcatt atggatgtgc agggtagcgg gtatatcgac tttggtgatg gcagtccgct
      961 taactttttc agctatgcag ggaaaaacgg tcatgcctat cgcagcattg gtaaggtgtt
     1021 gatcgaccgt ggcgaagtga aaaaagaaga tatgtcgatg caggcgattc gtcactgggg
     1081 cgaaacacac agtgaagcgg aggttcgcga gctgctggaa cagaacccgt ctttcgtctt
     1141 ctttaaaccg caatcttttg caccggtgaa aggggcaagt gcggtgccgc tggttggccg
     1201 tgcttctgtt gcctccgatc gttcaattat tccgccaggt actaccttgc tggcagaagt
     1261 gccgctgctg gataataacg gcaaatttaa cggtcagtac gaattgcgtc tgatggtggc
     1321 gctggatgtc ggtggcgcaa tcaaaggcca acacttcgat atctatcaag ggatcgggcc
     1381 ggaagccgga caccgcgcag gttggtacaa ccactatgga cgtgtctggg tgctgaaaac
     1441 cgccccgggc gcaggtaacg tctttagcgg ctgatgtgat attctgtatg ccgtttttac
     1501 ggatatgaca gggtgagggg taacctcacc ctttttaatt ctgaggtgct atgtctgtgg
     1561 taattagtga tgcatggcgt cagcgttttg gtggcacagc gcgtctgtat ggtgaaaaag
     1621 cattgcaact gtttgctgac gcgcatattt gtgtggttgg tatcggtggt gtcggttcct
     1681 gggcggcgga agcgctagcg cgcacgggga ttggcgcaat cacgcttatc gatatggatg
     1741 atgtgtgcgt caccaatacc aatcggcaaa ttcacgctct gcgcgataac gtcgggctgg
     1801 caaaagcgga agtaatggcg gagcgtattc gccagattaa cccggagtgc cgtgtaacag
     1861 tagtggatga cttcgtgacg ccggataacg tagcgcaata tatgaatgcc ggttattcgt
     1921 acgtgattga tgctattgat agtgtacggc ccaaagcggc gctgattgct tattgtcggc
     1981 gcaataaaat cccgctggtc acgaccggtg gcgctggtgg gcagattgac ccgacgcaga
     2041 ttcaggttac cgatctggcg aaaacgattc aggacccgtt ggcggcgaag ttgcgcgagc
     2101 gcctgaaaag cgattttggc gtagtgaaaa acagtaaagg taagctcggc gtggattgcg
     2161 tgttctctac cgaagcgctg gtgtacccgc agtcagacgg caccgtctgt gcgatgaaag
     2221 ccacggcgga agggccaaag cggatggatt gtgcctcagg atttggcgcg gcgacgatgg
     2281 tgaccgccac ttttggcttt gttgcggttt ctcatgcgct gaagaagatg atggcgaaag
     2341 cggcgcgtca ggggtaaaca atcaggtcgg atgcgacgcg aacgtcttat ccgacctggt
     2401 tctgtgctta aacctgcttc gccgcagcga taatcgcctc gcttaacgca ttcaacccct
     2461 ggctgcgtga ggcgctaagt tgcgcacgta atcccagctc atcaaacaat gccagcggcg
     2521 actgtgcctg caattcggcg gcggttttcc cctcaacggc agtcaacaac accgccaaca
     2581 ggccgcgcac aatgcgccct tcgctgtcgc caaagaaatg catcttgccg ttttcagcca
     2641 ctgtatatcc cagccagacg cggttttcgc atccggcaat ctctttagcc tgcgctttta
     2701 actcctctgg caaagccgga agctgtttcc ccagcatgat caactggcga tatttatctt
     2761 cccattgcgt caacggtgcg aaggtattgc gtaacgtttc tgcggttacg gttgtgccga
     2821 acggatgtcc ggcgaattgc gggtttgtca ttaatccacc aataattcca gcgcgcggtc
     2881 aacggcattc accagcgcat ccacatcact ctttgtatta tatggcgcaa atgaggcgcg
     2941 cagtgtgccg gttacgccta attctgccag tagcggctga gcgcaatgct gcccggcccg
     3001 cagggcaata ccgtactccg ccagcagcgt caccatatcg ctgtgatgaa cgccagcaaa
     3061 atcaaaggcc agcaggctgg aatcctggca gcgaaatgag cggaagccag gacgttttgc
     3121 cagcgcatct tccgccagcg ttgctaagct acggctccag ctttcggcct ggttgatatc
     3181 gtaatctgcc agccattcca gcgccgcgct taatcctatg acaccagcga catttggcgt
     3241 tccagcttcc agtttccacg gcgcagattg agtcgtgaag ccgtcaaaac tcacttcgtg
     3301 aaccattttg ccgccgccca gccagggcga catcgcttcc agcagttctg atttgccata
     3361 cagcacgccg atacctgtcg ggccatacag tttgtgacct gaaaaagcat agaaatcaat
     3421 atccagttgc tgaacatccg cggggaaatg caccgccccc tgagcaccat caaccatcac
     3481 caccatcccg gctgaatgag caaaggtaat cgctcgcgcc agatccgggc aaccgccagt
     3541 gacgttcgac atttgtccca gagcaagaat tcgactacga ggtgtgatca gttctggcaa
     3601 caaatcgaca tccggcagtc gctgcgcatt aagcggcaat ttcaccactt tggctccagt
     3661 ttgttgggcg accatcagcc aggggacgag gttggcgtgg tgttctgcca cgctgacaat
     3721 aatctcatcg cccggttgca gatgcggacg cgcatagcat tgcgccacca tgttgatgga
     3781 ttcagtggtg ccgcgcgtcc agacgatagt tttgtcatct ggtgcattca gtaattgcgc
     3841 cactttctcc cgtgcagctt cataacgcgc ggtcaggcgt tgggcttcgg caaactggct
     3901 gcgatggacg tttccggcgc tcaggctgta aaactgtcgg gtggcttcaa ccacggcttc
     3961 aggtttaagc gtggtcgcgg cgctgtcgag atagacgccc gcatcctgta gcgcgggaaa
     4021 ctgggcgcga aactgcgcgg gattaaaaac gttcatggta ctcctcggct tgatgcaccg
     4081 atcgtgacgc aaaattcact ttgatacaag gagagtgcta accatcagaa ttatctggat
     4141 atcctggcga agccgcgtca gtaacatatg ctgaatagtg ttaggcgtta attttagcct
     4201 gttaatcaat actattaaga tttattaatc gctttaagga gaagatgatg aaaaagactg
     4261 ccgcaattat ttctgcctgt atgctgactt ttgccctgag cgcctgttcc ggttcgaact
     4321 atgtgatgca caccaatgac ggacgtacca tcgtctctga cggcaaacca cagactgata
     4381 acgataccgg tatgatttcg tataaagacg ctaatggcaa caaacagcag atcaaccgta
     4441 ccgacgtgaa agagatggtc gaactggatc agtaattcgc gatcgcaagg taaaaaaaag
     4501 caccgcaatt aggcggtgct acattaatca ctatggacag acagggtaaa tgtacaggaa
     4561 gtgaaaaaag gtagctttgc taccatggtc tgaatcgcag accaattgca aacacaacaa
     4621 cacaacatca caaccgtaag ccaaaagttc accagaacac gcattccgat aaaacttttc
     4681 gttccggctc aggaagtgcc gccactatag gtatttgctg gtagaagctc aacggacaat
     4741 ttataatggc tcagattaaa aaaactaata ggttacatag tgtaatctaa ttgttaaatt
     4801 catttaacat caaagtttaa aagccatgtc taaacgatta ccaccgctaa atgccttacg
     4861 agtttttgat gccgcagcac gccatttaag tttcactcgc gcagcagaag agctttttgt
     4921 gacccaggcc gcagtaagtc atcaaatcaa gtctcttgag gattttctgg ggctaaaact
     4981 gttccgccgc cgtaatcgtt cactcctgct gaccgaggaa gggcaaagct atttcctcga
     5041 tatcaaagag atattttcgc aattaaccga agcgacgcgt aaactccagg cccgtagcgc
     5101 taagggggcg ttgacggtca gtttactccc cagtttcgcc attcattggt tggttccgcg
     5161 actttccagc tttaattcag cttatccggg aattgacgtt cgaatccagg cggttgatcg
     5221 tcaggaagat aagctggcgg atgatgttga tgtggcgata ttttatggtc ggggcaactg
     5281 gccggggcta cgggtggaaa aactttacgc cgaatattta ttgccggtgt gttcgccgct
     5341 actgctgact ggcgaaaaac ccttgaagac cccggaagat ctggctaaac atacgttatt
     5401 acatgatgcg tcgcgtcgtg actggcagac atatacccga cagttggggt taaatcatat
     5461 caacgttcag caagggccaa tttttagcca tagcgccatg gtgctgcaag cggctatcca
     5521 cgggcaggga gtggcgctgg caaataacgt gatggcgcaa tctgaaattg aggccggacg
     5581 tcttgtttgc ccgtttaatg atgttctggt cagtaaaaac gctttttatc tggtttgtca
     5641 tgacagccag gcagaactgg gtaaaatagc cgcctttcgc caatggatcc tggcgaaagc
     5701 cgctgctgaa caagaaaaat tccgctttcg ttatgaacaa taatttacgt agggtacgac
     5761 catgaccagc cgttttatgc tgattttcgc cgccattagc ggcttcattt ttgtagctct
     5821 gggcgctttt ggcgcgcatg tgttaagtaa aaccatgggg gccgttgaga tgggctggat
     5881 ccagaccggc ctcgaatacc aggcgtttca tacgctggcg atcttaggtc tggcggtggc
     5941 aatgcagcgt cgcatcagta tctggtttta ctggagtagc gttttcctcg cgttaggcac
     6001 ggtgctgttc agcggcagcc tttattgcct ggcgctgtcc catctgcgtt tgtgggcgtt
     6061 tgtcacaccg gttggcggcg tgagcttcct tgcgggctgg gcgttaatgt tagttggtgc
     6121 tatccgttta aagcgcaagg gcgtaagtca tgaataaggt tgtattgctg tgccgtccgg
     6181 gctttgaaaa agagtgcgcc gcagaaatta ccgataaagc cggccagcgg gaaattttcg
     6241 gttttgcccg cgtgaaagag aatgcgggtt atgtcattta tgaatgttat caacctgatg
     6301 atggcgataa gttaatccgt gagctgccgt tcagttcatt aatttttgcc cgccagtggt
     6361 ttgtggtggg ggaactcctg cagcatttgc cgccagaaga tcgtattacc cccattgtcg
     6421 gcatgttaca gggtgtagta gagaagggcg gtgaactgcg tgttgaagtt gccgatacca
     6481 acgaaagcaa agagttgctg aaattctgcc gtaaattcac cgtgccgcta cgcgctgcct
     6541 tgcgcgatgc gggtgtgctg gcgaactatg aaacgccgaa gcgtccggtt gtgcatgtat
     6601 tctttattgc accaggctgc tgctataccg gttactcata cagcaacaat aattcgccgt
     6661 tctatatggg cattccgcgc ctgaaatttc cggcagatgc gccgagtcgt tccacgctca
     6721 aactggaaga ggcatttcat gtgtttattc ctgcggatga gtgggatgaa cgcctggcga
     6781 acgggatgtg ggcggtggat ttaggcgctt gcccaggcgg ctggacctac caactggtga
     6841 agcgcaacat gtgggtttat tccgtcgaca acggcccgat ggcgcaaagt ctgatggata
     6901 ccggacaggt gacgtggctg cgggaagacg gtttcaaatt ccgtccgaca cgcagcaata
     6961 tctcctggat ggtatgcgat atggttgaaa aaccggcgaa agttgcggca ttgatggcgc
     7021 agtggctggt taatggctgg tgccgtgaaa ccattttcaa cctcaaactg ccgatgaaaa
     7081 aacgctacga agaagtgtca cacaatctgg cgtatattca ggcacagctt gatgaacatg
     7141 gcataaatgc tcagattcag gcgcggcagt tgtatcacga tcgcgaagaa gtgacggtgc
     7201 acgtccgccg tatctgggct gcggtgggtg gtcgtcgcga cgagcgataa caggcaaggc
     7261 cggggcgtcg tttccggcct gctatccttt ccgtcaggct gttaccaaag aagctgcaac
     7321 ctggcgctca tcagaaataa tgtgcgtgac ctcatccaga tgcccgatgc gggcttcccc
     7381 tgaacgatta aatttacttt tatcaatcaa taacaacgat tgcgcggcac gttttaatag
     7441 catcgatttg taatcagcgt tgatggcatt ggagtcccac agtgcgccgc tgctatcgat
     7501 cccttcacaa gaaaaaataa acagatcgat ttccagcgat ttaagctggg aaatcagcga
     7561 gggattgacg taacaaccat atttgcgctc aagtgtgccg ccggaactga tcagttgaat
     7621 gcgttcgcgt ttaccgagtt catggcaaat cggatggcta ttggtgaaga cctggatgtt
     7681 gatgtcaggc aactggcgtg ccagatacca gcaggttgaa cttgcgtcta aggctattac
     7741 catcccttct tcaatccacg cgagcgcctc gcgcgcgata tctgctttat gcgcataatg
     7801 gcttttcagc cggatgtgaa agggatcgcc actgtcttga ttttgacggt ggatatattt
     7861 agcgcgccca tgattgcgca gaattttacc ctgcgtctgt aattcattga gatcgcgacg
     7921 aatggtttct ttactgacct ttagctgttc agagagagct tccgtggtca ggctggtatg
     7981 gttcagcagc aggtcgacta tcgcttgctg gcgtgccgct ttcatatttt tcaccccatc
     8041 attcgataac cgcccgcgtc atgcgggcgt accggtatga gattacggtg ttaccccttt
     8101 ttttaaaaga atattcccgt acttcgctcg ttcgcctgtg ataacgatcg caaaggcttt
     8161 ttgtgcccgt tcataaaacg caaaacgatt gatgcggatg atgtcaggac agggggcttg
     8221 tagtgaaagc gcattacgat aacgtcgttc tacttcagga tctagagtgt caccttctac
     8281 cgccgccatc atcaccagcg gtggtgcata actgtccagc tcaaataacg ggataatcgc
     8341 ctggagcaag tcgctcacca acaggccatc agcgcggatc acctgcggtc ccatcgaatg
     8401 ggcgggaaag tgagcatcgg aaaaaataat ttcatctcca tgccccatct ctgccagcac
     8461 tttcaatagt tcgggagaaa ttaacggcga aattgttttc agcatttcac acctcctcta
     8521 taaattcagg ttcagtttgc gggtagaaat aacggtactg ataatgaatc tgcgcgcggg
     8581 cttcttccgg actgttaaat tcccctacgc cataccaacc aaacagcgca gctcctgcga
     8641 cggtcgtttc ggcatcgtcg agaactttta ccggaatatc aagcatattg gctttaatct
     8701 gattccacaa tgtgttgcga cttcctccgc cgactaacaa taattcagag gctttaaagt
     8761 gcccgatttt ttccagcatc tgtagattgc gctgtaattg cgtggttaac ccttccagcg
     8821 ccgcgcgata gaaatgcccc cgcgtggtat taagcgttac gccttgccag ccagcgttct
     8881 ggcacgacaa taaatcacac tgcattttca ctccatctgc gccaggcgcg atcaggcgag
     8941 cttcttcaat caatatttgc cagggagttt cagctgtcca gaacagtttt ctcacccatt
     9001 ccagcacgcc ggatgccagc cattgcatac ctgggttata caaccctgcc tggctatcca
     9061 gttcgcaagt agaaccggcg tactgactta acagcgaagt atcaacctgg gcgctgcgga
     9121 ccattaaaat ttcccatgta ccggaagaga gcacgggttc attttgttcg gcaccagcgc
     9181 caaaaagggc gaactgggta tcgtgacctg cggaaatcac cggtatgcca acgggtaagc
     9241 cgagcattgc tgcggcgctg ttctgtagcg taccaatctg ttcacccgct tccaccagac
     9301 gagggaagag tcggcgtgga ataccggtgg cttgtaaaat ttgcggactg aaatcgcgtt
     9361 gctggatatc cagcatctgg ctggttccgg ccatcgtaat atcagtagtg aattcgccgg
     9421 ttaaacggtg gttaatcagt gacgaaataa agagccaggc gtgcgcgcgt tccagcagtt
     9481 gtggatgatt ttctttcaac cacaccaact tatataacgt attgaaacta aaggctccga
     9541 ctccagaaat agcctgcaac tgctgtgcgg agatcaaccg ttcaatattg tccattaccg
     9601 cggctgttcg cggacatttc cagctaatga tcggatagag cagattgcct tgcttatcta
     9661 ccagagcgcc atccacacca aaggtggtga cggcgatacc gcggatatgg caatcagtca
     9721 gttcactatt gatttgccga cagcaatcag caaagcgttg caaaatggcg tctaaagacc
     9781 actggtgcca ggtgttgttt tccatcgcga tatcgctggc attaggcgtt gaggcgcggg
     9841 caacaatttt tccctgccga ttaaccgcga tggccctgac attggtcgcg ccacagtcga
     9901 gtaccaggat aacttcttgt ttcataatcg ctcctgcaat atagccggat aacattgctt
     9961 atccggctaa ccactcttgc ttgttcaggc ccggtagccg gagcgaccgg gcatcacatc
    10021 agggagtcat gtattaacgc ttgtacaacg ggccgtagtt ctggcaagcg cggtaatcct
    10081 ggccttcaat atccatgccg tgcgcggccc aggcagaagg acgatacact ttggtctctt
    10141 caacgttgtg catacatacc gggatacgca gcatggaggc aagagtgata aagtctgcgc
    10201 caacgtggcc gatggtcaga actccgtggt tagcgcccca gttcgccatt accgagtaca
    10261 catccgtaaa cggcccttta ccggtgaggc gcggtgcaaa ccaggtggtt ggccaggttg
    10321 agttggtgcg tttgttgagg atgtcatgca catccttcgg caattccacg ctccagcctt
    10381 ccgcgatttg cagtaccggt cccaggcctt tgatgatgtt gacacgagtc atggtgaacg
    10441 ggacgccgcc ttcggtaagg aagcgggaag agtaaccgcc gccacggaag tattcgtgga
    10501 tcgccgggca ccattcggta gcggcgaggc aagcgtcagc ctcttgctga gagatttccc
    10561 agtgcggctt catcgtcggt ttaccttcgc tgtcgcgttg tttacaggag ccgtccagcg
    10621 cagcagaacc ggagttgatc aaatggatga tgccgtgttc tgccagcccg tccagtttat
    10681 gccccgttac acgctcaatt gcttctggtg accagtaggt acgcacatcg gcaaatatct
    10741 gagcggtgcc ggtgagctgg tggcccatta acattgccac gccgttaaga ctgtcgtttt
    10801 cggtcgccac gacaaacggt ttgcgcacgc cattccagtc aaatgaactg ttgaggatcg
    10861 cttcagcggt atcgccattg ggatattgat cggtccagtg acgttgcccc tggaagcctg
    10921 cagcgatggc gttgtagcca agtgattctt ccacgcggcc aatatcggcc agtttgctgt
    10981 tgccttgcat catgtcgcgg atacacatcg ccatcagtaa actttcgcgc agaactgcgc
    11041 ggctttgctc ggcattgcgt tgatactgtt tgttattttc atcttcgcca tagcggaagt
    11101 ttttatcagc ccaggccagc gccatttcca gttcggcttc gtcgtaaatc ttctgatcga
    11161 tacggcgacg cagttcggtc atatccaccg cctggacttt cattcccagc caggattcaa
    11221 agaagttgtg atcaacgatg gaaccggcga tacccatcga aacgccgccc agcgacagat
    11281 agcttttacc tttcatgctg gcgactgcta aaccggcgcg ggcaaagcgc agcagttttt
    11341 cttcaacatc ggcaggaatc gatgtgtcat cggcatcctg aacgtcatga ccgtaaatgg
    11401 agaatgccgg gatgcctttc tggctgtgag ctgccagagc cgctgccagg taaacagcgc
    11461 cgggacgttc agtgccgtta aagccccaaa tggctttcgg gcgggttgga tccatatcga
    11521 tggtttcact gccatagcac cagcaagggg ttaccgtaat ggtgaggcct acattctgac
    11581 tgctgaattt ttcttcgcaa gcagcggctt cagccatacc cgcgatacag gtatcggaaa
    11641 tgacacactc gacggcagct ccacaggcat ggcgcagttt ctcggtcagc agtgcagccg
    11701 tggctttcgc catattcatt gtttgttctt caagcgactc acgaacaccc atgcgacgac
    11761 cgtcaataac cgggcggata ccaattttcg gtaagctgat ttttttcatt acagattcct
    11821 cactttattc ggaaaatatg ttcagttagt tgctgtttga gaacggaaac gggcaaagat
    11881 aaagatgacc gcgaagcaga gtgcggggat cagttcagca gtggggatgt tgcccgccgc
    11941 gtcactgaca aaacccatga ccggagtgac aataccgccg ccaatgatgg tcataacgat
    12001 gaaggacgaa ccgtatttgg tgtcctggcc gagattctta atgcccagcg agaagattgt
    12061 tgggtactga atcgacataa aggcgctgca taaagtcagg gctattaagc ccacatgacc
    12121 gccagcgaag gctgagatca ggcacagtgc catagcgatt aatgcgtagg ctgccaggac
    12181 tttgtgtggt gcgaagcgac tgatgagcca ggtaccggtg aaacgaccaa taaagaagca
    12241 caccatggtt ccggttaaat agttagcggc aaagcctgcg gtcatacctg gaatttcttc
    12301 tacagcgtag cgaatcaaat agctccagca ggccgtttgc gcgccgacat agcagaattg
    12361 cgctaatacc gcccagcgcc agtgacgaat acgcgccaga cgagaaagcg atgcggagaa
    12421 cgatccttgt ttggcgtcac tgtgattatc actctgcaat gccgggaatt tcgtcagcat
    12481 gatcagcagg gcgaccagta acacgatagc cacgatgatc atataaggtg tctgtaccga
    12541 taataccagg ctgtgtttat acgcactcaa ttgctctgga gacattttat cgagaacgtc
    12601 ttgcgattga tgtggcacgt tagacaaaat aagactttgt ccaaagacaa ccgcgataat
    12661 tgcgccaaac gagttaaatg tttgcgcaag atttaagcgg aagtgaccac tgctttccgg
    12721 ccctaatacc gtaacaaaag ggtttgcggc agtttccaga caacctaatc cagctgcaat
    12781 aataaatagg ccaactaaaa ataaggtgta gttcattatt tctgcagcgg gccagaataa
    12841 tgcagcgccc aaggcatata aaaataatcc ggtaataatc cctgctttat aactgagttt
    12901 tttcatcaat atcccagcag ggattgggat aatgaaataa ccaaagtaaa aggccgattg
    12961 gatcaggcca gcctggaaat ttgtcagcgt aaaagcctgc tggaattgag gtaataaaat
    13021 gtcgttaagg ttattggcta ccgcccaaag aaaaaacagt gagcacagca gcgcgaatgg
    13081 aataatgtaa cttctgcttt gccctgcatc tttatctacc gcacggtaac tctgcgtttg
    13141 tattgatgtg tttcccatag catcctctta ggttcagaag cttaagcacg taatcggtta
    13201 tgttttcaaa agtgcaaaac cagcgagttc gggcttcata tcagtgaata tttgcctgtc
    13261 agaaatgaac tcatggattc attttcaaat aaaaaacgct ctgtaatatg acggcggtca
    13321 cacttattag ttggga
//
LOCUS       DAZKGF010000137        13113 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZKGF010000137 DAZKGF010000000
VERSION     DAZKGF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.137"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(727..1092)
                     /gene="cdiI"
                     /locus_tag="ACHOQV_003771"
     CDS             complement(727..1092)
                     /gene="cdiI"
                     /locus_tag="ACHOQV_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease toxin immunity protein CdiI"
                     /protein_id="HFP9632068.1"
                     /translation="MNKYLFELPYERSEPGWTIRSYFDLMYNENRFLDAVENIVNKES
                     YILDGIYCNFPDMNSYDESEHFEGVEFAVGYPPDEDDIVIVSEETCFEYVRLACEKYL
                     QLHPEDTEKVNKLLSKIPS"
     gene            complement(1101..10865)
                     /gene="cdiA"
                     /locus_tag="ACHOQV_003772"
     CDS             complement(1101..10865)
                     /gene="cdiA"
                     /locus_tag="ACHOQV_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent inhibition toxin CdiA"
                     /protein_id="HFP9632069.1"
                     /translation="MHQPPVRFTYRLLSYLISTIIAGQPLLPAVGAVITPQNGAGMDK
                     AANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGNRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTT
                     GKPVMNADGSLQALEVTEGSITINGAGLDGTWSDAVSIIARATEVNAALHAKDLTVTA
                     GANRITADGRVSALKGEGNVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARE
                     GDIILSSSGKLVLKNSLAGGSTTVTGTDVSLSGDNKAGGNLSVTGTTGLTLNQSRLVT
                     DKNLVLSSSGQIVQNGGELTAGQNAMLSALHLNQTSGAVNAAENVTLTTTDDTTLKGR
                     SVAGKTLTVSSGSLNNGGTLVAGRDATVKTGTFSNTGAVQGNGLKVTATDLTSTGSIK
                     SGSTLDISARNATLSGDAGAKDSARVTVSGTLENRGRLISDDVLTLSATQINNSGTLS
                     GAKELVASADTLTTTEKSVTNSDGNLMLNSASSTLAGETSAGGTVSVKGNSLKTTTTA
                     QTQGNSVSVDVQNAQLDGTQAARDILTLNASEKLTHSGKSSAPSLSLSAPELTSSGVL
                     VASALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSGLI
                     TGDNGLTLNTASLSNPGKITADTLNVRATTLDGDGLLQGAAALALAGDTLSQGRHGRW
                     LTAGDLSLRGKTLNTAGTTQGQNLTVQADRWANRGSVLATGNLTASATGQLTSTGDIM
                     SQGDTTLNAATTDNRGSLLSAGTLSLDGNSLDNSGTVQGNHVTLHHRSTDNSGTVTGL
                     SGLTLHSADGLTNSGALLSQNSLVLSAGDVTNSGRIQGQNITLDASSLTSSGAVQSAL
                     DLALTLSGDVIAATGSKITALGDARLSGKVLGNQGLISAKTLEVNGDSLSNSGEISGV
                     NSLNVTLSGNLQQHGKMLTGGALNVNARDISNSGQLQGADNRITASSLANSGRVQGES
                     GLTLTLLNALTNQTSGVLLSQNVSALSAPALTNDGTIQGNGKTTLSAATQAHNSGKIL
                     SGGELTFTTPDYSGSGWLQATDLLLNVAKLAGNGTVMAANQATLTGNSLTNRGLFQAA
                     QLNVNTQTITNSGTLLGNQGLTIKGNNLNNAGGKVFSGGDMLAEMVSLSGAGQLVALG
                     NLTLKLTRGLTAQGVIAANKQLSVSSQGDITNGATLQGNGITLNAAGRLTNNGQLTAG
                     NGTTALSGSGIAMNASGSLQAGGDVSLTSRGDITLDAFTGTTGSLMLTAAGAVINTAL
                     LYAGNNLSLFASTIRNHHGDMLAGDSLVMQKDVSGAANAEVINTSGNIETTRGDITIR
                     TGHLLNQREGINETKSYIPVENVAVPDGANSVSVRVGDLGEDGWGYYVKSWSGTAGGG
                     FDAWAVPTEKGATRKFLTGTTRVDVGATGGDARISAGNNLLIDADKLDNTGSHLLASG
                     FVSLSGSQLNNQSFFGYTQDEYNVYRYYGKLAMIPNDGHLQYGDASADDRVTFTLSGA
                     PEYVTRDTGQALRAVIQAGKNVTAVFSSDISNTSTTSNAGRITNTLAAPEINTPAEKN
                     ISPGMAQLAPDGTETLTVTAPDWTDTITRLTIGSGTDLASGIVEGNYPLPSGNNGYFV
                     PSTDPDSPYLITVNPKLDGLGKVDSSLFTGLYDLLRMHPGQAPRETDPAYTDEKQFPG
                     SSYFLDRLGLKPEKDYRFLGDAAFDTRYVSNYMLNQIGGRYINGVGSDTDQMRYLMDN
                     AARAQKALGLKFGVALTADQVAALDQSILWYKAVTIKGQTVMVPEVYLSPKDVTLQNG
                     SIISGQNVHLAGGNVTNSGSTLMAQNNLTIDSADSLGNLESGLINAGGALGLKAMGDI
                     NNISATITGKTVRLESLAGNVNNLTRYSHWQLDAPEDSLALKHTYTGSIASVSAMDSL
                     DIRADKNISVTGAEISAGDRAALIAGNDLSLNAIDRVSSRRHANSESHQRSAGLTTIT
                     AGDSVMLSAGRDVSSQGAGIAAEDNITVRAGRDVNLLAEESVTGSSSYSKKKTVIDET
                     VRQQGTEIASGGDTTITAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLET
                     KKKSGGFLSKKTTHTISEDSATREAGSLLSGNRVTVNAGDNLTVEGSDVVADRDVSLA
                     AGNHVDVLAVTSTDTSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSAS
                     TIGSTAGNVSITAGKQAHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSG
                     LTVALSGTVGSAINNAVTSAQETKESSDSRLKALQATKTALSGVQAGQAAAMATATGD
                     PNATGVSLSLTTQKSKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGS
                     QLKAGGNTSLDAANDILLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFASVN
                     AAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGNRIIADVGHDLLISSQQ
                     DTSKYDSKQTSVAAGGSFTFGSMTGSGYIAASRDKMKSRFDSVAEQTGMFAGDGGFDI
                     TVGRHTQLDGAVIASTATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDK
                     FQGNMPGGMISAGGHSGHAEGTTQAAVAEGTITIRDRDNQKQNPADLSRDPAHANDSI
                     SPIFDKEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAAKEATGETLPANATEKQ
                     RQEYLAKLRDTPEYKKEQEKYGTGSEIQRGIQAATAALQGLAGGNLAGALAGASAPEL
                     AHLLKSTEKDPVVNAIAHAILGGAVAAMQGNNVAAGAAGAATGEMAARAIAGMLYPGV
                     KQSDLSEEQKQTISTLATVSAGLAGGLTGNSSASAAVGAQSGKNAVDNNYLSVSEKTE
                     LEIAKQTLKNSKNPAEREKAQQKYDALLEKDIASDKEVIAACGNGNAGSSACASARLK
                     VIASKEGYEDGPYNSKYSQQYADAYGQIVNLLDITSVDVQNQQQVKDAMVSYFMATLG
                     VDQKTAQGYVETTQGLEIAAASMTPLFGQAVANKITALVDKANKYPSGIGFKINQPEH
                     LAQLDGYSQKKGISGAHNADVFNKAVVDNGVKIISETPTGVRGITQVQYEIPTKDAAG
                     NTTGNYKGNGAKPFEKTIYDPKIFTDEKMLQLGQEAAAIGYSNAIKNGLQAYDAKAGG
                     VTFRVYIDQKTGIVSNFHPK"
     gene            complement(10878..12656)
                     /locus_tag="ACHOQV_003773"
     CDS             complement(10878..12656)
                     /locus_tag="ACHOQV_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP9632070.1"
                     /translation="MQHRQNNILTKTSLLFRAVSVPCYDIFRRARASSGICYLSLSVF
                     SGFFIPAFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALERSAPLTVIPSPEMSAG
                     TEGPCFTVSRIVVSGATRLTSAETDRLVAPWVNQCLNITGLTAVTDAVTDGYIRRGYI
                     TSRAFLTEQDLSGGVLHITVMEGRLQQIRAEGADLPGRTLKMVFPGMEGKVLNLRDIE
                     QGMEQINRLRTEPVQIEISPGDREGWSVVTLTASPEWPVTGSVGIDNSGQKNTGTGQL
                     NGVLSFNNPLGLADNWFVSGGRSSDFSVSHDARNFAAGVSLPYGYTLVDYTYSWSDYL
                     STIDNRGWRWRSTGDLQTHRLGLSHVLFRNGDMKTALTGGLQHRIIHNYLDDVLLQGS
                     SRKLTSFSVGLNHTHKFLGGVGTLNPVFTRGMPWFGAESDHGKRGDLPVNQFRKWSLS
                     ASFQRPVTDRVWWLTSAYAQWSPDRLHGVEQLSLGGESSVRGFKEQYISGNNGGYLRN
                     ELSWSLFSLPYVGTVRAVTALDGGWLHSDRDDLYSSGTLWGAAAGLSTTSGHVSGSFT
                     AGLPLVYPDWLAPDHLTVYWRVAVAF"
BASE COUNT         2762 a         3703 c         3290 g         3358 t
ORIGIN      
        1 aacagtaaag cgaaaaaaca atggagaact cgaagaagca gcatagcgtt tcgtagagtt
       61 agcccttgct aagggatgat tagttagtat tgtttgatgg ttattgctaa gtcttccata
      121 gttggtaaaa ttccgaaatg ttaaactttt gataaatggt attaaatcaa ggggttatat
      181 ggaaatcaaa gatgttttcg gggcgcaacc caaaagtgtg tgggagtata tctgtgagaa
      241 tggtcaagga ttgtatgtgc cagcatatca aaggcaatat tcttgggata aacctccagg
      301 ttgtgatgcc agttccacca tacgaagttt gaattcattc gaatagtttt tacgaggttc
      361 ttttcgccag tcctgtaatt ccatacttag atgtccgtct atatcagatg ggcgtctaag
      421 ttaccaattc tcgtctgatg gctacatacg gcggtcagtt tacgcttacg gttttgacat
      481 caattatctc cggtaatttg atgaatgaat gtcggtaatg ctaactgact gaaaatcgct
      541 gtgaagatga cttccccgta acgacacaac gtatgtcaac aggccgcaga tgctcacgtg
      601 gtttaacgcc ttccccggtg cgcttaacga cattagagat gtgccggtga ttaagcgtgg
      661 ggatggacat tgtggatgag cctgacggtt aaagaaagat cccggcgcag tggccggaat
      721 ctttactcag gatgggattt tagacagtaa tttgtttact ttttcagtat cttcagggtg
      781 taactgaaga tatttttcac aggccagacg aacatattca aaacaggttt cttcgcttac
      841 gatcactata tcatcttcat ctgggggata gccgacagca aattcaacac cttcaaaatg
      901 ttcgctttca tcataactat tcatatcagg aaagttacaa taaattccat ccagaatata
      961 agattctttg ttaactatat tttctacagc gtccaagaac ctattttcat tatacataag
     1021 gtcaaagtat gaccttatgg tccacccagg ttcagaacgt tcatatggca attcaaataa
     1081 atatttattc atagttactc ctatttcggg tggaagttac ttacaattcc tgttttttga
     1141 tcaatatata ctctgaacgt aacaccaccg gcttttgcat catatgcctg taatccattt
     1201 tttattgcat tggaataacc aattgccgct gcctcttgac caagttgcaa cattttctca
     1261 tctgtaaata ttttaggatc ataaatagtt ttctcaaaag gcttggcgcc atttccttta
     1321 taattaccag tcgtattccc agcagcatct tttgtgggga tttcatactg aacctgagta
     1381 atacctctga ctcctgttgg tgtttcagaa ataattttca caccattatc aaccacagct
     1441 ttattgaata catcagcatt atgtgcaccg ctaattcctt tcttctgcga atatccgtca
     1501 agttgagcta agtgttccgg ctgattaatt ttaaaaccaa tccctgaagg atatttattc
     1561 gctttatcaa ccaatgcagt aattttatta gccacagcct gaccaaataa tggtgtcatt
     1621 gaagccgctg caatttctaa tccctgcgtc gtctcgacat aaccttgtgc tgttttctgg
     1681 tcaaccccca gcgttgccat gaagtaactc accatcgcat ctttcacctg ctgttgattt
     1741 tgtacatcaa cactggtgat atcgaggaga ttgactatct gaccatacgc atcagcgtat
     1801 tgctggctgt atttcgagtt ataagggcca tcttcgtacc cttctttcga cgcaataact
     1861 ttcagtcttg cgctggcaca cgctgaacta cccgcattac cattaccgca ggcagcaata
     1921 acctccttat cactggcaat atctttttca agcaatgcat catatttctg ctgtgccttt
     1981 tcacgctctg ccggattttt actgttttta agcgtctgtt ttgctatctc aagctctgtc
     2041 ttttcagaca cgctcaggta gttattatca acagcattct tcccgctctg tgcgcccact
     2101 gctgctgatg cgctgctgtt tccggtaaga ccgccagcca gccctgctga cacggttgcc
     2161 agcgtactta tcgtctgctt ctgctcttca ctgaggtctg actgtttcac gcccggatac
     2221 agcatgccgg caatcgctct ggctgccatc tctcccgttg ctgcgcctgc tgcaccggca
     2281 gcaacattat taccctgcat tgccgctact gcgccaccca gaatggcgtg agcaatggca
     2341 tttactaccg gatctttttc tgttgatttc agcaggtgcg ccagttccgg ggccgaagct
     2401 ccggccagag cacccgctaa attcccccca gccagtccct gaagcgcggc tgtcgcggcc
     2461 tgtatacccc gctgaatctc actgccagtg ccatactttt cctgttcctt tttgtattcc
     2521 ggcgtatcac gcagtttcgc aagatattcc tgacgttgtt tctctgttgc attagcagga
     2581 agtgtttcgc cggttgcttc cttcgcggcc ttcaacgcat tcagttcccc ctgcgtccgc
     2641 gcgatatccg ccacctgact gcctatgtca ctgataagcc ccactgtctg cagacgcctc
     2701 tgctccttct ccttgtcaaa tatcgggctg atactgtcat tagcgtgcgc aggatcacgg
     2761 ctgaggtctg ccggattctg cttctgattg tccctgtccc ggatggtgat agtgccttct
     2821 gccactgcgg cctgagtcgt tccttccgca tgtccgctgt gacctccggc ggatatcatg
     2881 ccacccggca tgttaccctg aaatttatcc ccgaagctgc caccaccgct cagactgatc
     2941 ccactgtgac tgactttata atccgcttcg ttgtgaaggt cactgaaccc cagcgttccg
     3001 gtatccaggt ggtttttatc cggtgtggca gtggaggcaa tcaccgcacc atccagttgg
     3061 gtatgtctgc ccactgtgat gtcgaagcca ccatcaccgg caaacattcc ggtttgttca
     3121 gcaacggagt caaagcggct cttcatctta tcccgggagg cagcgatgta acctgagccg
     3181 gtcatggagc caaaggtaaa actgccgccg gcagccacgc tggtctgttt actgtcgtac
     3241 ttactggtgt cctgctggct gctaatcagc aggtcgtggc ccacatcggc gataatcctg
     3301 ttgccgttga cctgagcacc gttcagtacc gtatcccgac cactgttgat ggtgacggtt
     3361 ttaccgctgt ctgttgtggt ttcagtccac tcagtaccgt tacctttctc gctgcctttt
     3421 gccgcattaa cgctggcaaa gacgctgata ccggcaccgt taccaccggc accgatactg
     3481 acacccacgc caccgccact gctgctgttc ctgcccgttg ttttttgtgt gtttgcagcg
     3541 ccactcaaca gaatatcatt cgcggcatcc aggcttgtgt taccaccggc cttaagctgg
     3601 cttcctgcaa tcacaatatc tccgcggtta tcgcccctgt ttttgccggt tgcgacaaca
     3661 gacagattat tcccggcatt cagcgtactg ccggatactg tgtcactttc agaatgttgt
     3721 tgtgatttcg atttctgagt ggtgagcgac aggctgactc ccgtcgcatt cgggtcaccg
     3781 gttgcggtgg ccattgccgc agcctgtccg gcctgcacac cagacagcgc tgtctttgtg
     3841 gcctgcaggg ctttcagacg actgtcactg ctctccttcg tttcctgtgc actggtgacc
     3901 gcattattga tggcactgcc cactgtgccg gaaagggcaa ccgtcagccc gcttttcttc
     3961 tgctcaaatt tttcgtccac agtacgacgg tcatgccccg ggtcaaccac cacactgtca
     4021 ccggtaatgc tgatatcccg gttcgcaatc acatccgaac cgctgatatg agcctgtttg
     4081 cccgcggtaa tactgacatt accggcagtg gagccgatgg tactggcact ctgactctgc
     4141 gttgtcccgg cctcgcggcg gtcgtgcgtt gtcttactgc tgccgatggt gaagccgata
     4201 ccgccagtac ccatcagacc cgatttcttc gtttccttaa agcgccagga cgtatctgta
     4261 ctggtgacag caagaacatc aacatggtta cccgccgcca gtgacacatc ccggtcagcc
     4321 accacatccg aaccctctac cgtcaggtta tcaccggcgt taacggtcac gcggttcccc
     4381 gacagcaggg agcctgcttc acgggtggca ctgtcctcac tgatggtgtg ggtggttttc
     4441 ttactgagaa aacctccgct ttttttcttc gtttccagat agtgatagtc actttctgtc
     4501 gccgtggtca gggcaacatc acgaccggca ttcacgctga tattgccggt tgcggtaacg
     4561 gatgacgcaa cagcggtgat atcccgtcct gcagtaatgg tggtgtcacc accgctggcg
     4621 atttccgttc cctgctggcg aacggtttca tcaatgaccg ttttcttttt cgagtatgaa
     4681 ctgctgccgg tcacggactc ttccgccagc agattaacgt cgcgcccggc ccggacagtg
     4741 atgttgtctt ccgctgcgat acctgcaccc tgactgctca catcccggcc tgcactcagc
     4801 atcacactgt cgccggcagt aatggtcgta agcccggcgc tgcgctggtg agactcactg
     4861 tttgcatgac gtctgctgct taccctgtca atggcattca gactgagatc attgccggca
     4921 ataagtgctg cgcggtctcc ggcactgatt tctgcccctg tgaccgaaat gtttttatct
     4981 gcccggatgt ccagactatc catggcactg acagaggcaa tgcttcccgt ataagtgtgt
     5041 ttcagggcga ggctgtcttc cggtgcatca agctgccagt gtgagtaccg ggtgagatta
     5101 ttgacattgc cggcgagact ctccagccgg acagtcttac cggtaatcgt ggcactgata
     5161 ttgttgatat cgcccatcgc tttcagtccg agagcgccac cggcatttat aagcccggac
     5221 tcaaggttac ccagactgtc agcgctgtcg atggtgagat tattctgcgc catcagggta
     5281 ctgccactgt tagtgacatt ccctcctgcc aggtgcacat tctgaccgct gataatactg
     5341 ccgttctgca gcgtcacatc tttcggtgac agatacacct ccggcaccat caccgtctga
     5401 ccctttatcg tgacggcttt ataccacaga atactttggt cgagagccgc cacctggtct
     5461 gctgtcagtg ccacaccaaa cttcagaccg agcgccttct gtgcacgggc agcgttatcc
     5521 atcaggtagc gcatctggtc ggtgtctgaa ccaaccccgt tgatatagcg ccccccgatc
     5581 tggttcagca tatagttcga tacatagcgg gtatcaaaag cggcatcccc caggaagcga
     5641 taatcttttt ccggtttcag cccgagccgg tcaagaaaat acgatgagcc cgggaattgt
     5701 ttttcatcag tatacgccgg gtctgtttca cgtggtgcct gcccggggtg catcctgagc
     5761 aggtcataca gtccggtaaa cagactgctg tccacctttc cgagaccatc cagtttcgga
     5821 ttcaccgtaa tcagatatgg actgtcaggg tccgtggacg ggacaaaata accattgtta
     5881 ccggaaggca ggggatagtt cccctccacg atgccggaag ccaggtccgt gccactgccg
     5941 atcgtcagac gggtaatggt gtcagtccag tccggggctg tgacggtcag cgtctctgtg
     6001 ccatccggcg ccagttgagc cattcccggg ctgatatttt tctctgccgg cgtgttaatt
     6061 tcaggtgccg caagggtatt tgtgatccgt cctgcatttg aggtggtgga tgtattactg
     6121 atatcactgg aaaaaacggc agtgacattt ttgcctgcct gaatcacggc tctcagagcc
     6181 tgaccggtgt ctctggtgac atattccgga gctccggaga gagtaaaggt aacgcgatca
     6241 tcggcagaag catctccata ctgcagatgc ccgtcgttgg ggatcatggc cagtttgccg
     6301 taatagcgat acacattgta ttcatcctgt gtatagccaa agaaggactg attattcagt
     6361 tgtgaacccg acaggctgac aaacccgctg gcaagaagat gacttcccgt attatccagt
     6421 ttgtccgcgt caatcagcag attattcccg gccgaaattc tggcatcccc ccctgttgca
     6481 ccaacatcga cgcgtgttgt gccagtcaga aatttccggg tggctccctt ttcagtcgga
     6541 acggcccatg cgtcaaaacc accaccggca gtaccgctcc aggacttcac ataatatccc
     6601 catccatctt cacctaaatc gcccactctg actgaaacag aatttgcacc atcaggtact
     6661 gcgacattct caaccggaat gtatgatttt gtctcattta tgccctcccg ctgattcagt
     6721 aaatgtccgg ttctgatggt gatatcaccg cgggtggttt cgatattccc ggaggtatta
     6781 atcacttcag cattcgcggc accactgaca tctttctgca tcaccaggct gtcacccgcc
     6841 agcatatcgc cgtgatggtt ccggatggtg ctggcaaaca gcgagaggtt attgcccgca
     6901 tacaacagtg cggtgttaat gactgccccg gcggccgtta acatcagact acccgtcgtg
     6961 ccggtaaaag cgtcgagagt gatatctccc ctgctggtca ggctgacgtc cccgcctgcc
     7021 tgcaggctgc cgctggcatt catcgcaatc ccgctgccgc tcagtgccgt ggtgccgttg
     7081 ccggcggtca gctggccatt gttggtcagt ctgcctgcag cattcagcgt gataccgttg
     7141 ccctgcaatg tggcgccatt ggtaatatcc ccctggctgc tgacggagag ttgtttattt
     7201 gcggcgataa cgccctgtgc ggtcaaacca cgggtgagct tcagcgtcag gttaccgagt
     7261 gcaaccagct ggcccgcgcc gctgagcgaa accatttcag caagcatatc accgccgctg
     7321 aacaccttcc cgccggcgtt gttcaggttg ttccctttta ttgtcaggcc ctgattaccc
     7381 agcagcgtgc cgctgttggt gatggtctgc gtgttgacgt tcagttgtgc ggcctggaac
     7441 agaccacggt tggtcagact gttacctgtg agcgttgcct gattcgccgc catcacagtg
     7501 ccattaccgg caagcttcgc cacgtttagc agcaggtcag tggcctgtag ccagccactg
     7561 ccactgtagt ccggcgtggt gaacgtcagc tcaccgccgg aaagaatctt gccactgttg
     7621 tgggcctgcg ttgccgcgct gagcgtggtt ttaccgttgc cctgaatggt gccgtcattg
     7681 gtcaacgcgg gtgcggaaag cgctgatacg ttctggctga gaagcactcc gctggtctgg
     7741 ttggtcaggg cattcagcaa tgtaagagtc agcccgctct cgccctgaac acgacctgag
     7801 ttggccagcg aactggcggt gattcggtta tccgccccct gaagctgccc actattgctg
     7861 atgtctctgg cgttgacgtt taatgcaccg cccgtcagca ttttaccgtg ctgttgcaga
     7921 ttaccactga gtgtcacgtt caggctgtta accccactga tttcgccact gttgctgagc
     7981 gaatcgccat tcacttccag cgtcttcgcg ctgattaatc cctggttacc cagcactttg
     8041 ccgctcagac gggcatcgcc cagggcggta attttgctgc cggtggcggc aataacatcg
     8101 ccactcaggg tcagagccaa atccagcgca ctctgaacag caccgctgct ggtcaggctg
     8161 ctggcatcca gagtgatgtt ctgcccctga atccggccgc tgttggtcac atctcctgcg
     8221 ctcagcacca gcgagttctg gctcagcagc gcgccactgt tggtaagccc gtccgcgctg
     8281 tgcagggtca gaccgctcag ccccgtgacc gtaccgctgt tgtcagtgct tctgtggtga
     8341 agcgtgacat ggttaccctg gacagtgccg ctgttatcca gtgagtttcc atcaagggag
     8401 agcgtgccgg ccgaaagcag actgccccgg ttgtccgtgg tggctgcgtt cagcgtggtg
     8461 tcaccctggc tcatgatatc gccggtactg gtcaactgac cggttgccga agcagtaagg
     8521 ttaccggttg ccagcacgga accacggttc gcccatctgt ccgcctgcac ggtgagattc
     8581 tgtccctgcg tggtccctgc ggtattcagt gttttacccc ggagggagag gtcgcccgcc
     8641 gtcagccagc gtccgtgacg accctgtgag agggtgtcgc ctgcaagcgc cagtgcagcg
     8701 gcgccctgca acaggccatc gccatccagc gtggtcgccc tgacgttcag cgtgtcagcg
     8761 gtgattttac ccggattgct gagggaggca gtgtttaacg ttaaaccatt atcaccggtg
     8821 ataagcccgc tgttgcggat gtccggtata tccagcgtca ggtctgcagc actgtacagc
     8881 gtgccgttct gctgattatc aagcctctgt gtgttaacgg tgagtgaggc ctccccctgc
     8941 aacagaccgc tgttggtcag ggtctgtgac tgtgtattca gggcggaagc aacaagtacg
     9001 ccgctgctgg tcagttccgg cgcactgagg ctgagcgacg gggcgctgct tttcccgctg
     9061 tgggtgagct tttcactggc gttcagggtg aggatatctc tggcagcctg tgttccgtca
     9121 agctgtgcgt tctgcacatc cacgctgaca ctgttgccct gcgtctgcgc agtggtcgtg
     9181 gtcttcagac tgttgccttt tacagacacc gtgccacccg cactggtttc acccgccagt
     9241 gtggaagacg cgctgttcag catgaggtta ccgtcactgt ttgtgaccga tttttctgtg
     9301 gtggtcagtg tgtctgcaga agccacaagt tcctttgccc cggagagggt accgctgttg
     9361 tttatctgcg tggcactgag cgtcagcacg tcatcgctga tgagtctgcc gcggttttcg
     9421 agtgtaccgc tgacggtaac gcgggcactg tcttttgcac cggcatcacc ggacagtgtg
     9481 gcattgcggg cgctgatatc gagtgtgctg ccacttttaa tactgccggt gctggtcagg
     9541 tcagtggctg tgactttcag gccgttcccc tggacggcac cggtattact gaatgtcccg
     9601 gttttcaccg tggcatcgcg cccggcaacc agtgtcccgc cgttgttcag gctgccggag
     9661 ctgacggtga gtgttttccc ggcaacgcta cggcctttca gtgtggtatc atcggtggtg
     9721 gtaagggtga cattttcagc tgcattcacg gccccggaag tctggttcag gtgcagtgca
     9781 ctgagcatgg cgttctgtcc ggcagtcagt tcaccaccgt tctgtacaat ctgcccggat
     9841 gaagacagca ccagattttt atccgtcacc agacgggact gattcagtgt cagtcccgtt
     9901 gtcccggtaa cgctgagatt tcctccggct ttgttatccc ctgaaagtga gacatccgtt
     9961 ccggttacgg tggtactgcc gccggcaagg ctgttcttca ggaccagttt tccggaactg
    10021 ctcagtatga tatcgccctc gcgggcataa aggttaccaa gattaacccc gacaccactt
    10081 tcagtggagg tcagatgaat acgcctggcg tacattccac cgagagcgcc ggtatcaacg
    10141 gcaactttcg gcacattacc ttcgcccttc agggcactga cgcgaccatc tgcagttatc
    10201 cggttagcgc ctgcagtgac agttaaatcc ttcgcatgaa gcgcggcatt cacttccgtt
    10261 gcacgggcaa taatggatac ggcatcgctc caggtgccgt ccaggcccgc gccattgatg
    10321 gtgatactgc cttcagtcac ctccagcgcc tgcaggctgc cgtcggcatt catcacaggt
    10381 ttgcctgtgg tgagcgtcgc gtgcggcgtg ttgataaaac cacagccgtc acaggtgata
    10441 ccatacgggt tggcaaccat cacattcgcc gctttgccgg ccacttccgt atagccctgc
    10501 agcaatgaac ggttaccgcc agtcacttcg ttgatgatac ccttcgcttc cccgcccgct
    10561 ttcaggttcg ggttattctg tatcagtcca ccaagctgcg tcggattaag cttaccggtg
    10621 gcattattga gaatcagccc ttccttcccg acgttgtaat ccgtaaaccg gttatgcgaa
    10681 atcccggccc cgttcggcgt ggcaatgttc acgaccggca caccatttgc cgctttatcc
    10741 attccggccc cgttttgtgg ggtgatgacg gcccccacag ccggtaacaa cggctgcccg
    10801 gcgataatcg tactgataag gtaactcagc aggcggtaag tgaagcgaac gggaggctga
    10861 tgcatggtaa taatccctta aaacgcgacg gcaacgcgcc agtaaaccgt gagatggtca
    10921 ggggcaagcc agtccgggta aaccagaggc agtccggcag tgaacgaacc ggaaacatgg
    10981 ccactggtgg tgctgagccc ggcagcagca ccccacagcg tgccggacga gtacaggtca
    11041 tctctgtcag agtgcagcca gccgccgtcc agtgcagtca ctgcacggac tgtccccaca
    11101 tatggcaggg agaacagaga ccaggacagc tcatttcgca gataaccgcc gttattaccg
    11161 gagatatact gctccttaaa gccacgcact gaactctcac ccccgaggct cagttgttcc
    11221 acaccatgaa gacggtccgg tgaccactgg gcataagcgc tggtcagcca ccacaccctg
    11281 tccgtgacgg ggcgctgaaa actggcgctt aatgaccatt tccggaactg atttacgggc
    11341 aggtctcccc ttttcccgtg gtcgctttct gcgccgaacc agggcatccc ccgtgtgaat
    11401 accggattca gtgttccgac accccccaga aacttgtgtg tgtgattcag cccgacagaa
    11461 aatgaagtga gtttacggct gctgccctga agcagaacat catccagata attgtgaata
    11521 atgcggtgct gcagacctcc ggtcagtgct gtcttcatgt ccccgttacg gaacaggaca
    11581 tgcgacagtc ccagccggtg agtctgcagg tctcccgtgg aacgccaccg ccagccccgg
    11641 ttatcaatgg tgctgaggta gtcactccat gaatacgtgt aatccaccag ggtatagcca
    11701 tacggcagac tgacaccggc ggcaaaattc ctcgcatcat gtgacaccga aaagtcactg
    11761 ctccgtcccc cgctgacaaa ccagttgtca gccagcccca gcggattatt aaaggaaagg
    11821 acaccattta actgccccgt accggtattc ttctgtccgc tgttgtcgat gcccacgctc
    11881 cctgtgacag gccattccgg cgatgccgtc agtgtcacca ccgaccatcc ctcacggtca
    11941 ccgggcgata tttcaatctg taccggctcc gtacgcagac ggttaatctg ctccatcccc
    12001 tgctcaatat cccgcaggtt cagaaccttc ccctccattc ccgggaaaac catcttcagg
    12061 gtgcggccag gaaggtcagc gccttccgcc cggatttgct gcagcctgcc ttccatgacc
    12121 gttatgtgca gtacgccccc tgaaaggtcc tgctctgtca gaaaggcccg gctggtgata
    12181 tatccccggc gtatatagcc gtccgtcacg gcatccgtga ccgcggtcag ccccgtgata
    12241 ttcagacact gattcaccca cggtgccacc agtctgtcgg tttctgcaga cgtcagtcgg
    12301 gtggccccac tgacaacaat gcgtgacacc gtaaagcagg gaccttcagt accggcagac
    12361 atttccggag acggtatgac agtcagcggc gcactgcgct ccagcgcatc acgctgacgc
    12421 tggttttcat ccagcaactg ctgctgttgc tgctgaattg cactgcggtc acccggagac
    12481 agcatggctg ccggagacga aaatgcgggg ataaaaaaac cagaaaaaac ggagagggag
    12541 agataacata tccccgaaga ggcgcgggcg cgccggaata tatcataaca ggggacagac
    12601 acagcccgga ataaaagaga cgttttcgtc agaatgttgt tctgccggtg ctgcatgttc
    12661 tccccgtatt ctggcctgtg taatgtcagt gtaaattcat gtcaaaaatg tgtcagcggg
    12721 aaatttacac ctgaaaaagt ctgtcatgaa acagctttat gacaagatat caaaaaaaat
    12781 gtcaaaaacg tgatctttct tcagcttttc agaatattga gagcctagag gtcaatataa
    12841 attctgggac acatatgcat ttaccattaa aaccggcaca acaggctgtt cagtctcaat
    12901 ccatgctttc tgttttatcg atctcagaat atcggcagac aaataaaaaa ggaatgtcga
    12961 gagagctaac tctagatcac tctccacctc acaagccatt tacaaaaatg atacaggcat
    13021 atctttggtc cctgccacaa ccagacattg acaatccaat ataaccccaa atatatttcg
    13081 gatacgaagc ttgggattta tacagacatt tac
//
LOCUS       DAZKGF010000138        12822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZKGF010000138 DAZKGF010000000
VERSION     DAZKGF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.138"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(53..352)
                     /locus_tag="ACHOQV_003774"
     CDS             complement(53..352)
                     /locus_tag="ACHOQV_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9632071.1"
                     /translation="MTYEVIITSEAEQQIINLHRYITEKAGSAIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGNKVFIAGVYHGGQSYESDFL"
     gene            complement(358..615)
                     /locus_tag="ACHOQV_003775"
     CDS             complement(358..615)
                     /locus_tag="ACHOQV_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP9632072.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRAGK"
     gene            751..1029
                     /locus_tag="ACHOQV_003776"
     CDS             751..1029
                     /locus_tag="ACHOQV_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632073.1"
                     /translation="MAHDTRFYNSDNSAAPASRHGRRSHAFKSDWLQHPPCTEEQAEW
                     LIQCYRRRGYEFEKALSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            1031..2080
                     /locus_tag="ACHOQV_003777"
     CDS             1031..2080
                     /locus_tag="ACHOQV_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9632074.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLAAWMIDVIRHAMNDTQERE
                     LSLAELCWWAVCHQVADALPESVLRRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLKFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            2093..2467
                     /locus_tag="ACHOQV_003778"
     CDS             2093..2467
                     /locus_tag="ACHOQV_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9632075.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKSGERYRRAVALIVRQQ
                     QLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            2464..3285
                     /locus_tag="ACHOQV_003779"
     CDS             2464..3285
                     /locus_tag="ACHOQV_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9632076.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKQGVPVFRNCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            3513..3710
                     /locus_tag="ACHOQV_003780"
     CDS             3513..3710
                     /locus_tag="ACHOQV_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9632077.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            3861..4913
                     /locus_tag="ACHOQV_003781"
     CDS             3861..4913
                     /locus_tag="ACHOQV_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP9632078.1"
                     /translation="MLSTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRQYTELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRATGVELES
                     ERFLQTVKEIRDDFCR"
     gene            5002..5724
                     /locus_tag="ACHOQV_003782"
     CDS             5002..5724
                     /locus_tag="ACHOQV_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9632079.1"
                     /translation="MAVVCSVILVCSPINIFIEKYKLSLVPGMVILVTRNIRELFRTY
                     TDRVKVADINHDIVSQYLEGGRRRMSSPQACFPHYLTSGIHQPVLAEALITNHGFPSA
                     PSGDLSTMTGLSCLSLFANDESLSSFLSRCLSNTSDKVRAIIQSDISVNWKLGVIALQ
                     LHMSESLLKKKLKDEGNSFSRLLLEERMRGAVNMLCFQYGRGAIVAEKCGYSSLSYFI
                     SVFHRYYGVPPDKYVSLHMVDF"
     gene            6802..8769
                     /locus_tag="ACHOQV_003783"
     CDS             6802..8769
                     /locus_tag="ACHOQV_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9632080.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSDGEGTVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGASAFTTGADGTYSESAGASENSLRWGVGK
                     PLYQDLVSRTKAALAKNPKNRLLAVVWMQGEGDAAVGTHAQHPGLFSAMVNQFRTELA
                     GQASQSTGGSASAVPWVCGDTTYFWKQRHAEGYASVYGGYKGKESQNIFFVPLMTDEN
                     GANVQTNDPAEDPDLEAVGYYGSKWRNDQKTWTSVDRASHFSSWARRGIISDRLATAI
                     LVHAGRTAEFITGKQPDTVKPTGPSGEGPEREPEAPVSNRTLMSLLASGEDLASQGWR
                     YYHKPASGDSVDKNIAEAVVSDEGATGGKALQLNKPESHIWFLEHDAAGQGAELLKKG
                     GRVSVRFKVPGALVPNQFALGIYWQLSSLPEGVTLSEEGNDMLMSFFLQTDATNLNAM
                     YHKKQNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPASGAAD
                     KLQLTGISKAATYTLLIDSVKVEVNNADAAA"
     gene            8920..9102
                     /locus_tag="ACHOQV_003784"
     CDS             8920..9102
                     /locus_tag="ACHOQV_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9632081.1"
                     /translation="MTFLNQLMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLIP"
     gene            9140..9385
                     /locus_tag="ACHOQV_003785"
     CDS             9140..9385
                     /locus_tag="ACHOQV_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9632082.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDA
                     ILDELLGAPAVPEPEGIAGDGSASDGGEPTPDSDMMM"
     gene            9462..9677
                     /locus_tag="ACHOQV_003786"
     CDS             9462..9677
                     /locus_tag="ACHOQV_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9632083.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            9681..9908
                     /locus_tag="ACHOQV_003787"
                     /pseudo
     CDS             9681..9908
                     /locus_tag="ACHOQV_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            10984..11517
                     /gene="rrrQ"
                     /locus_tag="ACHOQV_003788"
     CDS             10984..11517
                     /gene="rrrQ"
                     /locus_tag="ACHOQV_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9632084.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            11815..12282
                     /locus_tag="ACHOQV_003789"
     CDS             11815..12282
                     /locus_tag="ACHOQV_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9632085.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     EQANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCHGT
                     VREATSTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAKEYIRTQCIN"
     gene            12306..12530
                     /locus_tag="ACHOQV_003790"
     CDS             12306..12530
                     /locus_tag="ACHOQV_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632086.1"
                     /translation="MKKLLVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT         3253 a         2919 c         3606 g         3044 t
ORIGIN      
        1 cttttataag tatttcttgg ttatttaagt tttttgttag tgaaaatata aactataaaa
       61 agtcagattc ataactttgc cctccatggt aaacgccagc gataaagact ttattgccat
      121 caacggcaaa agcaatgatc gttctgtggc ggaagtgagt tacccgcatt ccctgtcgaa
      181 tatcatcgcg tttattgccc cgatgcggga atgtggaaaa accatcaaga taatcaagaa
      241 gcgcgttggc ataattgtca gcaatggcgc tccctgcctt ctctgttata tatctgtgca
      301 ggttgattat ctgctgttcg gcctcagaag taatgatgac ttcatacgtc atgcagatta
      361 cttcccggct cgaatcgcgg cgcgaacctg tgaaatggag cgtccgttgt ttgggttttc
      421 gcggatagaa tcgagagatg gggcggctga atgcgttaac cacgcttcga ttgctttatc
      481 gcgctcattc agtgcgcgaa gcccttcacg aatgacctca ctttctgaag cataggcacc
      541 ggaagccaca cgggcgcgca ccatgtcagc catctcgtta gttaatgtaa tgctgaattg
      601 ttgggttgta cgcatggtaa acctcacgga gtaggataga acactattcg atgatagcac
      661 actgcctgtt gacgacacca gaaatcagat acaatattgc cgcacgccag cctgaacaac
      721 tggcacctgc tgcgccagca gagacaaccg atggcgcacg ataccagatt ttacaattcg
      781 gataactccg ccgcccctgc cagcaggcac gggcggcgtt ctcatgcatt caaatctgac
      841 tggttacagc atcccccatg cactgaagaa caggccgaat ggctgatcca gtgttaccgc
      901 aggcgcggat atgagtttga gaaagccctc agcttcgatc gtcgtcactg gataatctcc
      961 gtcaggctcc cttattccga acgcccaccg cgtccgtccc gcacattcca gcagcgcatc
     1021 tggaggtaac gtgcgggtat tacttcgacc tgttctggta ccggaactcg ggatggtgct
     1081 ccttaagccg ggccgtgaat caatgtcagc attccataac ggcagaatat tggtggaacc
     1141 ggaaccgaaa aacatgcgcg gtctgccgtc cggagtcgtt cctgccgttc gacagccgct
     1201 ggcagaggat aaaacattac tgccattttt cagcaacgaa cgggtgattc gtgctgcggg
     1261 tggcgctggt gcactgtctg actggttatt acgtcacgtg aaatcctgcc agtggccaca
     1321 cggtgattat catcacagcg aaaccgtcat tcaccgttac ggtaccggcg cgatggtgtt
     1381 gtgctggcac tgtgacaacc agctgcgcga ccagacatca gaatcactcg atcaacttgc
     1441 tcagcagaat ctggcagcct ggatgattga tgtcatccgt cacgcaatga atgacacaca
     1501 ggagcgggaa ctgtcgctgg cagagttatg ctggtgggct gtttgtcatc aggtggctga
     1561 tgcgcttccg gagtctgtat tgcgtcgttc gctgggatta ccagcggaaa aaatccgctc
     1621 cgtataccgt gagagtgaca tcgtaccggg agaacagaca gccatcagca tactgaagca
     1681 gcgcacaaaa aatattgcgc tgccacttca cgtccaccag caacaaaatc caccacagaa
     1741 aaaaacggtt gtcagtatcg ctgttgatcc ggagtctcct gaatcgttca tgaggcggcc
     1801 taaacgtcgc cgttgggtta atgagaaata cacgcgctgg gtaaagacac agccgtgtgc
     1861 gtgttgtggt aagcctgctg acgatccgca tcacctgatt ggtcatggtc aggggggaat
     1921 ggggacaaag gcccacgata ttttcacgct accgttgtgc cgggagcacc acaacgaact
     1981 tcatgcagac ccgctgaagt ttgagaaaaa gtacggctct cagattgagt taatttttcg
     2041 ttttcttgat cacgcctttg cgactggcgt gctcgggtaa aagaggtgac tgatgctcat
     2101 agatttggtt ttaccttacc cgccgacggt gaacacttac tggcgacgcc gtggcagcac
     2161 atattttgta tcaaaatccg gggagcgtta tcgccgggca gtggcactta ttgttcgcca
     2221 gcagcagttg aaattaagcc tgtccggacg gctggcaata aaaattattg cagagccacc
     2281 ggataagcgt cgtcgtgacc tggacaatat tctgaaagca ccgctggatg cgctgacgca
     2341 cgcggggttg ttaatggacg atgagcagtt tgatgaaatc aatattgtac gtggccagcc
     2401 agtatctggt ggacgtctgg gggtgaagat ttaccccata atgcttgaag ggcaggtcaa
     2461 aaaatgaaac tggaagattt accgaaatac tactccccaa aatcccccgg cctgactgat
     2521 gcatcggcct caacgtcgaa agatgcgctg agtatcactg atgtgatggc cgcgcagggc
     2581 atgacacaga atcgggctga gatggggttt tctgcgttcc ttgggaaaat gggcattagt
     2641 atgaatgaca gagagcgggc aacagaattg ctgacagaat atgcactcag tcggtgtgat
     2701 cgcgtggcgg cgttaagaaa actcccggca gaaataaaac cggcagtgat gcgtattatg
     2761 gcttcgtatg cgtttgaaga ttatgcccgt agcgcggcga gcaaaaaaca gtgcccctgc
     2821 tgtcacggaa aaaaatttat tgaaagcgag gtttttacaa acaagatcca gtatccggat
     2881 ggtaagccgc cagtgtgggc aaagtgcaca aaaggcgtgt atccgtctta ctgggaggaa
     2941 tggaaaaaag tcagggaggt ggtaaaagtt gcctgtccgg agtgtggagg gaagggagag
     3001 gtatccactg cctgtaaaga ttgtcgtggg cgcggtgttg ccattcatcg tgaagagtcg
     3061 gaaaaacagg gtgtgccggt tttcagaaac tgtcagcgtt gtggtgggcg tggctatgaa
     3121 agattacctt caacggaggc atttaatgcc atatgtaatg taaccgatgc catatctctc
     3181 gatacatgga aaaaaacagt taaacgtttt tacgatacgc tggtggtgca gtttgatatt
     3241 gaagaagcat gggcagaaca acaactgaaa aaggtgacca gatagctttg ttgatttttc
     3301 ccgaatctgt ggtaaatttg ccctaacgat gggtgtttta tgcctgacgt tagaagattt
     3361 tttacacccc gccgcctggc gggttttttt atgactgaaa tcacgccagt acagtaaacg
     3421 cgctggtggc ggtgaatacc ggtctttcag cttgctggct ttttcgacaa gagttattgg
     3481 tgtgtcacgt taaccggaaa agggaaaaag acatgctgaa acagcaggat atgaccgaaa
     3541 ccgccagagt ggtgtttaat gaattaagcg tcaccgaacc ggcgactgtc ggggagattg
     3601 cacagaatac ttacctttca cgtgaacgct gccagttaat actgacccag ctggttatgg
     3661 cggggctggc agactatcag ttcggttgtt acagacgcct tcagtcctga aggctttttt
     3721 atttgtggta aatgggcggc tggtgggtgt taggggcact caccagccat ctgctcatgc
     3781 gtccggatca caagcaaacc tcaggcccac tgctttgcgc aaaagcagaa tgagcctatc
     3841 agagacaggc ttaatgatcc atgcttagta ctgtaaaaat atccagttgt gagttaatca
     3901 acgccgactg cctggaattt atccggtcgt tacccgaaaa ttctgttgac ctgatagtca
     3961 cggacccgcc gtactttaaa gtgaagcctg agggctggga taaccagtgg aagggcgacg
     4021 atgattacct gaagtggctg gaccagtgtc ttgcgcagtt ctggcgggtg ctgaaacctg
     4081 ccggaagtct ttacctgttc tgtggccatc gcctggcatc tgacattgaa atcatgatgc
     4141 gtgaacgctt cagtgtgctg aaccatatta tctgggcgaa gccgtccgga cgctggaacg
     4201 ggtgcaacaa ggaaagcctg agggcgtatt tccccgccac agagcgcatt ctgttcgcgg
     4261 aacattatca ggggccgtat cgtccgaaag atgccgggta tgaggcgaag ggcagggcac
     4321 tgaaacagca tgtgatggcc ccgctgattg cttactttcg tgatgcacgt gctgccctgg
     4381 ggataacggc aaaacagatt gctgatgcca caggaaagaa aaacatggtg tcgcactggt
     4441 tcagtgccag tcagtggcag ctaccggacg aaagcgatta tctgaaatta caggcgctgt
     4501 ttgcccgggt ggcagaagag aagcatcagc gcggagaact ggaaaagcca catcaccaac
     4561 tggtcagcac atacagtgaa ctgaaccgac agtatacgga actgcagagt gaatataagc
     4621 atctgcggcg gtatttcggt gtgacggcgc aggtgccgta caccgatgtg tggacgcata
     4681 aaccggtgca gtactatccc gggaaacatc cgtgcgaaaa accggcagaa atgctgcagc
     4741 agataatcag cgcgagcagt cgtccgggtg acttggttgc agatttcttc atggggtcgg
     4801 gctcaaccgt caaagcagcg ctggcactgg ggcgtcgtgc aactggtgtt gagctggaga
     4861 gtgaacgttt tttacagaca gtgaaggaga tccgggacga tttttgcagg tgacagaggt
     4921 tgaggaaagg ttgaagggag gatcatttgt tattgttgta tataattatt ctaccccttt
     4981 cctgagtcag gtaaatatat aatggcagtt gtctgttctg taattctggt ttgttccccg
     5041 atcaatattt ttattgagaa atacaaactt tcacttgtgc cgggcatggt cattctggtc
     5101 accagaaaca tcagggagct ttttcgcact tacacagacc gggttaaggt tgctgatata
     5161 aatcacgata tagtcagtca atatctggaa ggtgggcgaa ggcggatgag ctccccacag
     5221 gcatgttttc cgcattatct cacttctggc attcatcagc ctgtactggc agaggcgtta
     5281 attactaatc atggatttcc cagtgctcct tctggtgatt tgtccacgat gacagggctt
     5341 tcatgtcttt ctctgtttgc caacgatgag agcctgtcat catttttgtc aaggtgttta
     5401 agcaatacaa gcgataaagt cagggcaata attcagagcg atatatccgt aaactggaag
     5461 cttggtgtta ttgcgttaca actgcatatg agcgaaagct tgttaaagaa aaagcttaaa
     5521 gatgaaggaa acagcttcag ccgcctgttg ctggaagaga ggatgcgggg ggcagtaaat
     5581 atgttatgct ttcaatatgg acggggagcg atagtggcag aaaaatgcgg ttattcaagc
     5641 ctgtcttact ttatatcagt attccacagg tattatggag tacctcctga caaatatgtg
     5701 tctctgcata tggttgactt ttaatttcat cctgctggcg gatggagtgc tggtggtcgg
     5761 cattgtggtg gtgctttttc ctttggttaa aaaggacatg ccaggtagtt agcgggtatc
     5821 agtcatgccc agaaaatttt aaatgtctca caattcagac ggttgacagt tgtctgtttt
     5881 gcggggagtt tgttaaaaga aactggcatg gtgaatcccc ctgagcggag gggcgactgg
     5941 tgcatcaggt gttatctttg atacccagga aagtgaacgc gcgggttcgg tggcaccggg
     6001 ctgaactcac cgggaggcac ccggcactat gcgcatgatg tacagatacg cggcttcagc
     6061 ccctctccgg aggggctttt ctgtgcctta tgcatcacag tttctggaac cctgagtact
     6121 acagtatcag tcagggtgct atatttgcag atgtgatgaa agcctgccgg caggcagggc
     6181 gtatcggaaa tgacccagta agagaaaacg ttgactcaga taccggtgct gagttaccgg
     6241 gaaaccggca tcacatgacc gctatccttc atggctctct caggagagcc tttttactgg
     6301 agaaaaccgc ttccccgtaa aatgccgtgt tgctcacaat tcagtcagtt gaaggttgtc
     6361 tgcccgactg agaatttgtt aaaaaatttc tgcatgatga atccccctgt gcggaggggc
     6421 ggctggtgta cggaatggtc tctgatgatc gttagcgaga atgacgcggg ttcggtggca
     6481 ccgggctgaa ctcaccggga ggcacccggc atcatgtaat gaacggtaca tagcgctact
     6541 cttcagcccc tctccggagg ggctttctta tggataaaaa aagcccgcat ggtgagatgc
     6601 gggcagcaag gaataaacaa caaaacgtga agtaatcaat ttttcagctg gcgaataata
     6661 cccgacagta atcactctgc gcaactgcgc ggcctttttc gtattgcggg ctgtagtctt
     6721 cctcctgcca ttgtcctgta acttccggac ttcagcccgc ccctcatctg actcacaaca
     6781 ttatcccgac cgggaggatt catggcattt aaacactatg acgtggtcag ggcggcgtcg
     6841 ccgtcagacc ttgcggagcg actgactcaa aaactgaagg aggggtggca gccatttggc
     6901 agtcctgtcg ccatcacgcc ttataccctg atgcaggcca ttgcggcgga aggtgatgtc
     6961 accacacctg tggtggtgaa gccgtcggat ggagaaggca cagttatcag cgccaccagc
     7021 gacccggagt attactttgt tgtggttctg gcagggcagt caaacggcat gtcgtatggt
     7081 gaaggccttc cgctgccgga gacatatgac cgtccggacc cgcgtattaa gcagctggcg
     7141 cgccgaagta cggtgacacc gggaggtgcc gcctgtaagt ataacgacat tattccggcg
     7201 gaccattgtc tgcatgatgt gcaggacatg agccgtctta accatccgaa agcggacctg
     7261 tcaaaggggc agtacggaac cgtggggcag gggctgcata tcgccaaaaa actgctgccg
     7321 tttataccgg cgaatgcggg cattctgctg gttccgtgct gccgtggtgc ttcggcattc
     7381 acaacgggtg cagacggcac atacagcgaa tcagccggtg catcggaaaa ttcactgcgc
     7441 tggggtgtgg gtaagccgct gtatcaggat ttggtgagcc ggactaaagc cgcactggcg
     7501 aagaacccga aaaaccgtct gcttgcggtg gtgtggatgc agggagaagg tgatgcggca
     7561 gtggggacgc atgcgcagca tccggggctg tttagtgcga tggtgaatca gttcagaacg
     7621 gagcttgccg gtcaggcatc acagagcaca ggaggcagcg catcagcggt accgtgggtt
     7681 tgcggggaca caacgtattt ctggaagcag cgtcatgcgg aagggtatgc atcagtatac
     7741 ggagggtata agggtaagga gtcgcagaac attttctttg taccgttaat gacggatgag
     7801 aatggtgcga atgtgcagac caatgacccg gcagaagacc cggacctgga agccgtcggt
     7861 tattacggtt caaagtggcg taatgaccag aagacctgga catctgtgga cagggccagc
     7921 catttcagtt catgggctcg ccgtgggatt atttccgacc gtctggcaac ggcgattctg
     7981 gtgcatgccg ggagaaccgc tgaattcatt accggaaaac agcctgatac ggtgaagccc
     8041 accggacctt ccggtgaagg cccggagaga gagccggaag ccccggtcag taaccgaacc
     8101 ctgatgagtc tgctggcgtc cggtgaagac ctggcatcac agggctggcg ctattatcac
     8161 aaaccggcga gcggagacag tgttgataaa aacatcgctg aggctgtggt cagtgatgag
     8221 ggtgccaccg gtggtaaggc cctgcaactg aacaaaccgg aaagccacat ctggtttctg
     8281 gagcatgatg ccgccgggca gggggcagag ttgctgaaga aaggcggacg tgtgagcgtg
     8341 cggtttaagg tgccgggcgc actggtgccg aatcagtttg ccctgggtat ttactggcag
     8401 ttgtcgtccc tgccggaggg agtgacgctg tcagaggaag gcaacgacat gctgatgtcc
     8461 ttcttcctgc agacggatgc gacgaacctg aacgcgatgt accacaagaa gcagaatgcg
     8521 aagctggata cgttcggggt ctttgataac ggatggcaca cactggcttt tgagtttgcc
     8581 ggaaacaaca gcattcaggt gacgccggta ctggatgaga aacggggtac accgttcaca
     8641 ctggtgaaat ctccggcatc aggggcggcg gacaaactgc aactgacagg catatcaaag
     8701 gcggcgacat atacgctgct gattgacagt gtgaaggtgg aagtgaacaa cgcggatgcc
     8761 gcggcatgat aaaaaaggcc gccagcaatg aaggaaaggg atgaactttg tccactacat
     8821 gaatgctggc ggctggtaaa acgaccatga tgacaccgaa gtctgcggtg tttttctaca
     8881 aaaataagta aatcatgtca actatcacgg tgagaaacca tgacatttct gaaccagtta
     8941 atgctgtact tctgtacggc ggtctgtgtg ctgtatcttc tttcgggtgg gtacagggct
     9001 gtgcgcgatt tctggcgcag gcagattgat aaaagggccg cagagaaaat cagcgccagt
     9061 cagtcagccg gaagcaaacc cgaagagccg ctcattccgt agtcactttc ttaacaacac
     9121 ctttcaacga gaaaatccta tgtcagaaat aaaatcgctg gtcactgctg aagcagtgaa
     9181 ggaagtcctg cgctctgaag aagtcagaag cgcactgaaa cagaaactcc gccagaacct
     9241 tgagtcgcgt cttgatgcag aagtggatgc cattctggat gaactgctgg gcgcaccggc
     9301 agttcctgag ccggaaggca ttgcgggtga cgggagtgct tcagatggcg gtgaacccac
     9361 acccgacagc gacatgatga tgtaagcatg cgcaagggac catcggtgtg tgtcggtggt
     9421 ctttatattg ttgtgagctt ccggattgcg ggagacgggg tatgtaccag atggaaaaaa
     9481 tcacaacagg tgtgtcatac accacgtcag cggtgggaac gggctactgg ttcctgcagt
     9541 tgctggacag ggtttccccg tctcagtggg cggcaatagg cgtgctgggg agtctgctgt
     9601 ttgggctgct gacatacctg actaacctgt atttcaagat taaagaagac cgtcgtaagg
     9661 cggcgcgggg agagtagacg atgaataaac aatacgaact ggttgttaaa gggataaata
     9721 attacccgga taagattact gttactgtgg cactggaaat tgccgggcag ccgtcgttgt
     9781 tgtcgccata tgtggcgatt agtcttgacc gtactgaagg tgccaagctg gaattttacg
     9841 aagctgaggc gaaaaagaag gcgaagcagt ttttcatgga tattgctgcc gctttatgtg
     9901 aaggggatga acagtcacgg gaaaagcgcc tctgttcaga agagcggtat accatacaaa
     9961 ttaacaatgc atataacacg atacagtcag aaaaagatga tatcgaatca cgcattgaaa
    10021 aactggagaa cagcgtcgtt gaactgaata aaaaactgtc ggtgcagatc ccttccgaag
    10081 gtgaaaaaaa acgccgtgat gagcagttcg cggcgtttta cgattattgc cggaaagtga
    10141 tgagcagaaa tctcgcagag tgtttccgta ttcaaaaaaa cagagagcgc tctgcaaata
    10201 gtgagattct ggataatttt ttaaagggga cgcatctcgc cagtgattgt ttcattgctc
    10261 attcttttgg tggctttcat gaggccatca aacatgatgt tgagagaagt ccaggtatcc
    10321 tctgttccat tcccgtaagc atcgaaatag acacaaatac gataaccggt gaactcgtta
    10381 ccgccggagt cggtatattc cgtcatgagg ggaacgatgc caaaggttcc tttacaaaaa
    10441 tcgggccgtt ctttctgaag gtagagcgat agtttgttaa gtacccatcc gacagctcgc
    10501 tcattttggt ttattttcgt atcgcgaaac aaaaattcga agcggccaga tgacgcatgg
    10561 gtgcaaccaa ataccggggc gtattcatca acctttggtg ttcctgaaaa catgcagtcc
    10621 gtgctctcga atgcagatga attgctgttg atccacttaa caaaagaaaa aaaggtctga
    10681 atcgcttgtc tgtctgaata ttcaacaaaa tcctcaaggt gtgtctcgat cagttccggg
    10741 tgtttacgaa agtcataata ttgaccggga cgatatttat gagtggttgt tttatcccat
    10801 ggttgtgctc tgcgtccctg taaacggagg tctgccgctg gatagataag cataagtaat
    10861 ctcctggtgg aatgtaatcc acgatcatcc ggggaaatta agaacccgcc agtgcccacc
    10921 actggtgggg tgaaggctta acatatccag ggattcggaa ccgataaatc ctgataaata
    10981 tccatgaaca ccaaaatcaa atacggcctg tcggctgccg ttctggcgct gattgccgca
    11041 ggtgcgcctg cgcctgaaat cctcgaccag tttctggatg aaaaagaagg caaccacacc
    11101 acggcatacc gtgatggcgc gggtatctgg accatctgcc ggggtgccat catggtggat
    11161 ggtaagcctg tgattcctgg catgaagctg tcgaagggga aatgcgaccg ggttaacgcc
    11221 attgagcgtg ataaggcgct ggcatgggtg gagaaaaaca tcaaagtgcc actgaccgaa
    11281 ccccagaaag cggggatcgc gtcattctgt ccttacaaca tcggcccagg taagtgtttt
    11341 ccgtcgacgt tttataaacg aattaatgca ggcgatcgca ggggagcgtg tgaagcgatt
    11401 cgctggtgga ttaaggacgg tggcagagac tgccgtatcc gctcaaataa ttgctacggt
    11461 caggtatccc gtcgcgacca ggagagtgcg ctggcatgct ggggtatcga caggtagtag
    11521 aatattttgc tgaaaaatga cgttgaccaa cgcggacgga taacacgaaa tcctgcgaac
    11581 tggcaaaacc taagtgaata aaagtaaaaa ccccgtttgt tggcagcaag cggggttttg
    11641 tgtttcctga ctccggaaaa gtcaaaggag aaagtgtgtt tgattttagc aaactgattc
    11701 gggagattcg agtgatggct gaaaaattat ccacctggaa gttcatcctt atctggctgg
    11761 tgtttgtgat tatggcctcc ggttatttca tcggtcagat acgctggtgg tgaaatgaac
    11821 cgcgttctgt gtgtggtcat cattgccttg ctggtggcct gtggtgcgct tagtctgggg
    11881 ctgaatcatt accgcgataa cgccatcacc tacaaagcgc agcgcgataa aaaagccaga
    11941 gagctggaac aggcgaatgc caccattact gacatgcagg tgcgccagcg tgatgttgct
    12001 gcgctcgatg caaaatactc gagggaatta gccgatgcga gagctgaaaa tgaaactctg
    12061 cgtgctgatg ttgccgctgg tcgtaagcgc ctgcggatca acgccacctg ccacggtacc
    12121 gtgcgtgaag ccaccagcac ctccggcgtg gataatgcaa ccggcccccg actggcagac
    12181 accgctgaac gggattattt catcctcaga gaacggttga tgacaatgca gaagcagctg
    12241 gaaggggcga aggaatatat ccgcactcag tgtattaact agtatttttg ttatccggag
    12301 aatgcatgaa gaaattactg gtaaccgtag aaccttttca gggaacaatt ccgttccgta
    12361 ttttgcagcg tggtcgtgtt cttgttgaag gttcgttcag tggtaaatgt acgcaattac
    12421 actcccggac ctttcaggtg aatgccacga atgaagagct aaccgttgag tgtacgatga
    12481 atgccgctaa atgccgcatg gtatccgctg cattacagcc agtgtgttga gcgaccttat
    12541 tatccatgcg cggtattgtc gccgtattcc cgtattaaca gagaccgcag cccgacaggg
    12601 agactcctct gcgcgagtgt gcggggataa tcaaaaacga tacacaccgg ggtttaccgc
    12661 gttaacggag cgcggcgttg tcccctcatg gtcgctggtc ggtgcgatgg tggaagaaac
    12721 tggattttgt tgcaactgat aaccattatc attttcgcgg gtcctttccg gcgatccggg
    12781 ccgttacggg gcggcgacct cgcgggtttt cactatttat ga
//
LOCUS       DAZKGF010000139        12775 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZKGF010000139 DAZKGF010000000
VERSION     DAZKGF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.139"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(31..750)
                     /gene="dcuR"
                     /locus_tag="ACHOQV_003791"
     CDS             complement(31..750)
                     /gene="dcuR"
                     /locus_tag="ACHOQV_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFP9632087.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(747..2378)
                     /locus_tag="ACHOQV_003792"
     CDS             complement(747..2378)
                     /locus_tag="ACHOQV_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9632088.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2558..2788
                     /gene="yjdI"
                     /locus_tag="ACHOQV_003793"
     CDS             2558..2788
                     /gene="yjdI"
                     /locus_tag="ACHOQV_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFP9632089.1"
                     /translation="MDQALLDRGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2800..3072
                     /locus_tag="ACHOQV_003794"
     CDS             2800..3072
                     /locus_tag="ACHOQV_003794"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9632090.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3299..3595
                     /gene="ghoS"
                     /locus_tag="ACHOQV_003795"
     CDS             3299..3595
                     /gene="ghoS"
                     /locus_tag="ACHOQV_003795"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP9632091.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRFKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3623..3796
                     /gene="ghoT"
                     /locus_tag="ACHOQV_003796"
     CDS             3623..3796
                     /gene="ghoT"
                     /locus_tag="ACHOQV_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFP9632092.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(3915..5432)
                     /gene="lysS"
                     /locus_tag="ACHOQV_003797"
     CDS             complement(3915..5432)
                     /gene="lysS"
                     /locus_tag="ACHOQV_003797"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9632093.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPLK"
     gene            complement(5669..7126)
                     /gene="dtpC"
                     /locus_tag="ACHOQV_003798"
     CDS             complement(5669..7126)
                     /gene="dtpC"
                     /locus_tag="ACHOQV_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFP9632094.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(7185..9332)
                     /gene="cadA"
                     /locus_tag="ACHOQV_003799"
     CDS             complement(7185..9332)
                     /gene="cadA"
                     /locus_tag="ACHOQV_003799"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFP9632095.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSSWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYEDMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9412..10746)
                     /gene="cadB"
                     /locus_tag="ACHOQV_003800"
     CDS             complement(9412..10746)
                     /gene="cadB"
                     /locus_tag="ACHOQV_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFP9632096.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTAGTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAGSGAPFAISASTILGNWAAPMVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(11112..12650)
                     /gene="cadC"
                     /locus_tag="ACHOQV_003801"
     CDS             complement(11112..12650)
                     /gene="cadC"
                     /locus_tag="ACHOQV_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFP9632097.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT         3532 a         2966 c         2958 g         3319 t
ORIGIN      
        1 aattattact tacagataac cagcgatccg ttattggcaa tattgtttca gcagtgagta
       61 gtgttctgcc tgaatacggt aacggtaaac cggacgccct gtgacgccat aatggatact
      121 ggtgaacaag atgtggcagt tgaccagcca gatgaggtat ttacggcagg aaacacgcga
      181 gatgttaacc tcgttggcca gctcgtcggt tgaaaactca tagtcctgat gcgcgtcaat
      241 ccactggcac agcgtgcgta acgtctgcgg cgtcaagcct tttggcaagc gacgaggatc
      301 ctgttcgttg gagctgctgc cgtggattag ctgatcaagc tcggcctggt cataatactg
      361 atgtttttcc agcgccattt tcttttgccg ccagccggtg agcgcctctt caaagcggga
      421 agcctggaag ggtttgatca ggtaatccac gacaccgtaa tgcagcgaat ctttaatggt
      481 tgccgcatcg gctgcggagg agatgacaat cacatcactt ttgcaacgcg cgttatgcag
      541 gacaggcagt aaatcgagcc cgttctcttt ttgcatatag atatcgagca atatcaggtc
      601 gataggcgta tcgctattga agataatctc tttggctttc tccagcgtcg aagccgttcc
      661 acagcattga aagcctggga tttgtgccac gtatcggcga ttcagctccg cgaccattgc
      721 gtcgtcatcg ataattaata cattgatcat ctgttcgacc tctccccgtc ccagggtatc
      781 tggacaaaaa attgtgtgaa aatcccgggt tccgattcca cggcgatgct gccgccgaga
      841 ttttctacct gttgtttgac aagtgctaaa ccgacgcctc gctcgcttcc ttttgtcgag
      901 acacctttgt caaaaatgtg atcgattttg tcgggtgcga tccccggtcc atcatcgtta
      961 acttcacagt gcagccagcc gtgacggtag tgcaatgtta cgctaatttc gccaccgggt
     1021 tccggtccta atgcctccag cgcgttttct atcagatttc ccagcgtggt aatcagcgtc
     1081 gcgacctggt cttcactgcc gctgtctggc aactggcttt cactgtttaa aatcagcgta
     1141 tggcctaaat cggtcgcgcg gttaatctta ctgattaaaa aaccagcgat aaccggagat
     1201 ttgatcttac ccagcagaga gccaatctct tcttgatagt tattggctgt tttgagaatg
     1261 taatcttcca actgcttata actcttcaga tgcaataatc cgagaatcac gtgcaattta
     1321 ttcataaatt cgtgggatcg ttcacgaagt gcgtcagcat agttgaccag accatcgagt
     1381 cgctgcatca gtttacgtac ttcagttttg tccctgaagg ttgaaatggc accgatgata
     1441 acgccattac tgcgcaccgg aacggtgttg atcagtaata gccggtcttt aatcgtaatc
     1501 tcttcgtcgc gccgcggggt accgtcgcgt aacacttccg agacatctac cacctgtgac
     1561 cacgagtggc ttagcgtcga cagcttctca tcgtcctgcg acttacggta attcagcaat
     1621 tcttgtgcgg catcgttgat cagcgtgact tcaccgcgat cgtccacggc aacgacgcct
     1681 tctttgatgg actgcaacat agcctggcgt tgctcaaaca gcgtggagat ttcgtagggt
     1741 tccaggccga aaaggatttt tttcagtacc ttaaccagaa tgcaggtgcc aatcagcccg
     1801 accagcatgc caaataatac cgaccagatg atgctccagc gactgtcatt gatctgttgg
     1861 gtcacgcgac ttaactcaag gccgatcgcc accacgccaa tttgtttatg attttcatcg
     1921 tagatggggg taaatacgcg taaagcctgc gccagaaaac cgcgattgat agcgacattt
     1981 tcttcgccat tcagcgcttt aaggatgtca tcacctttaa atggctgacc aatacgctgg
     2041 gcttcaggat gcgagtagcg aagactttgc atatcggtaa cgacaataaa cagcagatcg
     2101 ttgcgtttgc gcacggcttc cgcgatggcc tggattccac tctcctgtgg ttttttctgc
     2161 aagccctgac ggatttccgg cgagtcggcg agggtacgcg ctactgccag tgccttgtta
     2221 gctagcccgt ctcgcgtcat atcactgatt tgcgagaagt aaatcagatg caccaccaat
     2281 agcaccgaga acagtaccgc gctgaccatt aagatcactg tggtactcaa tttcatcgga
     2341 cgtttgcgta acatgcggta gggcaatgaa tgtctcatca gcttccttgt gtgacaaatt
     2401 tcttaagcat tatctctgat gaggcgggta attcaaaggg agtaagaatg attggcatat
     2461 aggggaagag actctggcaa cggaaactgc cagtgctgta tgaagattcc agggctatgc
     2521 ttatagtgaa aaccatactg atgagagagg gaaggtcatg gatcaggcgc tactggacag
     2581 gggttatcgc tgttataccg gcgaaaagat cgatgtctat ttcaacactg cgatatgtca
     2641 gcattctggc aattgcgtac gtggcaacgg caagttattt aatctcaaac gaaagccgtg
     2701 gatcatgccg gatgaagtcg acgtcgccac tgtggttaaa gtgattgata cgtgcccgag
     2761 cggcgcgctg aaataccgtc ataaataagc gagggtaaaa tggaaatacg cgaaggccac
     2821 aataaatttt acattaatga cgaacagggc aagcaaatcg ctgaaattgt ctttgtgccg
     2881 accggagaga atttagcgat tatcgaacat accgatgtcg atgaaagcct gaaagggcaa
     2941 gggattggta aacagctggt tgcgaaagtc gtggaaaaaa tgcgtcggga aaaacgaaaa
     3001 attatcccac tatgcccatt tgcgaaacac gaatttgata aaacgcggga gtatgatgat
     3061 attcgcagtt gatgggagag tacagagtca cgatattttt catgctctcc gcggtgtgat
     3121 gcaggagagc atctgaaggg tagggggatg cacaaagaat gggcagagag cgcgtttttt
     3181 tgtcccaagt catccccttt actgagcaaa aaaaagaata tctcctatat gagaatcatc
     3241 aatcggggtt aataagtttt gcgtccccag agcgtttaat attgatagga gtcatattat
     3301 ggaaggtaaa aacaagttca atacttatgt tgtttctttt gattatccat catcttattc
     3361 ctcagtgttc ttaaggttaa gatcattgat gtatgatatg agtttctcct ctatcgtggc
     3421 tgatgaatat gggataccac gacaactgaa tgaaaactcc ttcgcaataa cgacatcgtt
     3481 agccgcaagt gaaatcgaag atttaatcag gtttaaatgc ttagacttac cggatattga
     3541 ctttgacctc aacattatga cagttgatga ctatttccgt cagttttaca agtagccagg
     3601 aaggataaga agaacataaa aaatggcact attctctaaa atattaattt tttatgtgat
     3661 tggtgtgaac atatcctttg tcattatctg gtttatctca catgagaaaa cacatattcg
     3721 tttacttagt gcatttctgg tcggaataac ctggccaatg agtctgcctg tggcattact
     3781 tttttctctc ttttaggagc tgtagttgag tggacccgct ggttatcttg aaacgaagcg
     3841 tctctcctca aaactggatt tgcggaatga cgaacgaagc agtcgcctga ctgcttcgtt
     3901 cattaaagtg aaatttattt cagtgggcgc atcgccggga agagaataac gtcgcgaata
     3961 gtatgactgt ttgtaaacag cataatcatt cggtcgatac caatacccag accagcggtt
     4021 ggcggcagac catattccag cgcagttacg taatcttcgt catagaacat ggcttcgtcg
     4081 tcacctgcag ctttagcgtt aacctgttcc tggaaacgtt cagcctgatc ttctgcgtcg
     4141 tttaattcgc taaaaccatt accaatttca cgaccaccga tgaagaattc aaaacggtcg
     4201 gtgatttccg ggttaacatc attacggcgc gccagcggtg acacttctgc cggatattcc
     4261 gtgataaagg ttggctgaat cagatgtgct tctgccactt catcaaagat ctccgtgaca
     4321 atgcgtccca acccccagct tttctctacc gtaattccga tagattcagc taatgcttta
     4381 gcagcatcaa aattatccag atccgccata tcggtttctg gacgatattt tttgattgct
     4441 tcgcgcatgg tgagtttttc aaacggtttg ccgaaatcaa acacatgctc gccataagtg
     4501 actttagtgg tacccagtac ctcttgtgcc agagtgcgga acagtgattc cgtcagttca
     4561 atcaaatcgt ggtaatccgc atacgccatg tagagttcca tcatcgtgaa ctcaggatta
     4621 tgacgaacag aaataccttc attacggaag ttacggttga tttcgaatac ccgttcaaaa
     4681 ccacctacaa ccagacgttt cagatatagc tccggcgcga tacgcaggta catatctaaa
     4741 tccagagcgt tatgatgggt aataaacggg cgagcagatg ctccacctgg aattacctgc
     4801 atcatcgggg tttctacttc cataaagccg cgagcgacca tgaactgacg gatagcggcc
     4861 agaatttttg aacggacaac aaacgtttga cgggatttat cgttagcgat gaggtccaga
     4921 taacgttgac gataacggac ttcctgatcc tgcagaccat ggaatttatc tggtaaagga
     4981 cgtagtgctt tagtcagcag gcgcagctca gtacagtgaa tggaaagctc acccgtttgc
     5041 gtcttaaaca gcgtaccgcg ggcaccgata atgtcaccca gatcccattt tttaaactga
     5101 tcgttataaa caccttctgg caggctatct cttgcaacgt acagttgaat acggccaccg
     5161 acatcctgca acgttacaaa ggaggctttc cccataatac gacgggtcat cattcggcca
     5221 gcaaccgaga cttcaatgtt taaggattcc agttcctggt tatccttcgc atcaaactct
     5281 tcgtgcaact ggtcagaggt atggtcgcgg cgaaaatcat tgggaaacgc cacaccttgc
     5341 tgacgtagtg ccgccagttt ttcgcggcga tttctcagtt catcgttaaa atcaatagcc
     5401 tcattggctc cccgtgtttc tcgttcagac attttggttc ctctaaatcc agctttcaat
     5461 ttagcgtaga taaagagaca gatcggtaca ctgtattatc tgcctcgact attaataact
     5521 cagtttatct tatcgaaact aacgcttacc tcaagttgat atcactaata aataaccata
     5581 aaaatggttt aaatgactta ttaatctcga tttgctagtg tgataaactg aaagttaatt
     5641 tattcataac caattgtttt tacgacagtt aatcgttgct ctcctgtatc atattcgtcg
     5701 gcgtgctaaa cagaaaacgg gtagcaaagg ccaataccac gatgatcgcg acacaagcca
     5761 acgtccactc tcccatctga gaaaagaatc gctggtaagc ggcaattgcc ataccgctaa
     5821 tttgcgactc tgtcgtctgc tgtgccacga cgcctgccag ccagttggcg accgcgcctg
     5881 ttgccagcat ataaataccg gttaatacgc cagacatttt cagacgcgtg atttgcgcaa
     5941 tcgccaccgg gtcaataaag agttcagcaa agcccattag cgccagcccg gatatcatca
     6001 cgcccattga cgcttgaccg ccagccgctg catgtcgggc atcaaatgcc agcaacataa
     6061 agccacaagc catcagcagt aggccaaagg caaacttcag ccagacgcgc aatgctgaat
     6121 tgccgcggct ttctggcgac gccagccagg ccagaacaac cccagcgagc atcaccgcaa
     6181 tagcattcac cgactggaat agtgctgtag gtacttcaat attgaatgcc tgacgattca
     6241 caaagcgatc gataaacaag ctgatggtac tgccgccctg ttgtgccagt acccagaaca
     6301 acgtcccgac aaacatcaac aatacaattt gccaaagagc acggcggtgt tcggggaatt
     6361 tgatcatcat gcgggcaatg atttgtgctg caatgaggca aacgatcgcc agcaaatatc
     6421 cggaccagtc gttctccagc agcagggtaa aaaatactgg ggctaaacag agcatcacca
     6481 ctaaccagct ccatactggt aaggcaaatt tgacgctagt gagcgctttt ttatccatac
     6541 tacgcgtgga ctggaaatga cgatgaccac ttaagaaaat caacaaaccg ataaacatgc
     6601 cgcctcccgc aagggcaaag ccaacatgcc atccatacca ctgagcagcc aggccgcagg
     6661 cgatgggcgc ggcgatagaa ccgatattgc ccgcagcata tagcagcgaa aaaccgccat
     6721 cacgtcgatg atcgttctcg tcgtagagct cgccaagcaa acagctgatg tttgatttga
     6781 ataaaccgta gccacaaata atgattgcca gcgccagata caggctaaag gttgaatttg
     6841 tatcaatgcc cagcaccaca tggccaaggg tcattaacag cgcgccggca atcactgcag
     6901 tgcggttgcc gagcaggcgg tcggcaagcc agccgccgag aataggggta acgtaaacca
     6961 gagaagcata tgcgctgaac aggctgatgg catggttatc atcaaaacca agctgatggg
     7021 tgagatagag aatgagtaag gcacgcatgc cgtaaaaact gaagtactcc cagatttgga
     7081 tcgccacgat atagtatatc gcgcgcggct gtgagggtgt tttcatgtgt tctccttatg
     7141 agcaaaaaag ggaagtggca agccacttcc cttgtacgag ctaattattt tttgctttct
     7201 tctttcaata ccttaacggt atagcggcca tcagcctgac ggtatgcacc gtgaatatcg
     7261 gtttcaaagc ccggatagtg agcgccgatt tcacacagca tctgcaggaa ctccagaacc
     7321 ggacggcttt cttcggtgat catttcaccc ggcattacca gaggaactcc cggcggatac
     7381 ggaaggatca tattggcgtt aatacgacct accatttcgt cgaggtaaac ttcttcggtc
     7441 ataccgtgca gctctttctg gaacgccgca tacggagtca taaccattgt cggcagaact
     7501 tcaaatgcgc gatacatcag atccggcaga ttgtggtgaa caatcagttt gtggatattc
     7561 tgagccagtt cctgaatacg catatcttca tagaattcag gatcttcacg atatagagac
     7621 ggcagcatgt ttttcacacg caggttcagg tcgaacgcac gtttgaagtc agtcagagca
     7681 cgcagcaggc tcaatgcttt ggtcttatca ataccgatgc tgaacaggaa cagcaggtta
     7741 tacgggccgg ttttctcaac aacgatgcca tgttcgtcga ggtatttcgc cacgatgctg
     7801 gccggaatac caaagtcgct catggtgccg tctttttcca tccccggggt cagcagggtg
     7861 actttgatcg ggtcaagata catgtgctca ttgtcgatgt tcttgaagcc gtgccaggag
     7921 ctgtcagaac gcagcggcca gcattcagtg gtatcgatat gatccggctg ccatacatca
     7981 aagaaccagc catcagattc cgttctcaga cgtttgatct ctttacggaa cttgatcgca
     8041 cgttcaatgg aaccgttgat cagacgctta ccagcattac ctttcatcat cgccgcagcg
     8101 gtttcagtgg acgccacgat accgtagtgc ggagaggtgg tggtgtgcat catgtaggct
     8161 tcgttaaagg tttcttcgtt tacgtcacct ttaacgtgga tcatggaagc ctgagagaac
     8221 gccgccagca gtttgtgagt ggactgggtt tcgtaaatca ctttcccttc tacacggcca
     8281 ccgctcatac cgcatttacc ttcgtaaatc ggcgagaagt tggtgtaagg cacccacgcg
     8341 gagtcaaagt ggatggattt cacatccagc gttttcttga tgaagtcggt gttgtacagc
     8401 agaccatcat aggtagagtt ggtaattacg gcatgtaccg gccaggttgc gtttggtgtt
     8461 tctttaacgc gcttagcaat ggttgcgtgc tggaattcac tctgtgggat accaccaaga
     8521 ataccgtaag cgttacgggt cgggcggaaa tagattgggg taacatcgct catcatcatt
     8581 aggtgggtta gcgatttgtg gcagttacgg tcaatcagaa tggtgctgcc tgccggagca
     8641 gagtacatac cgacaatttt gttcgcagtg gaagtaccgt tggtcaccat gtagctgcgg
     8701 tctgcgttaa agacgcgagc gatatactgt tccgcttctt tgtgtggacc gctgtgatcc
     8761 agcagagaac ccaattcaga tactgaaatg gaaatatcag atttcatggt gttcggacca
     8821 aagaaatcat agaacaggct acctaccggg cttttctgga atgcagtacc gcccatgtga
     8881 ccaggagtac agaaagtata tttaccttca cgaacatatt taaacagtgc tttggtcagc
     8941 ggaggcagaa tagtgttgat atattcgtca gtggtctgct tgatcttgtt agcaatatca
     9001 tcagcagcac ccagcgcata ttcaaagaag ctaatctgta aacgcagatc gttcaggctt
     9061 acatcgagtg tggaatacgt attagcgaac gcgtacaacg gcaggttctc gttcattttg
     9121 ctaatttctt cgcacagctc gagattatat ttatcccagt caaaaataac accgcacaga
     9181 cgcgcattgt tttcgatcag ttttaataag tcgtcacggt cattcgggta aacaatctgg
     9241 aagttcagac gttcaagcgc gcgatgaagt tcacggatgg gttcttcttt aaaataaaca
     9301 cccatgtgat tcaatattgc aataacgttc atagtcatat ctccaggtaa aaaaggcccc
     9361 tcccaacaca tgggacaaaa tgaaaggagg agcctcggaa aatactttta attaatgtgc
     9421 gttagatgct gtgtggttat ccattgagtg gctctggcgc tcgtgcattt tgcgagcgta
     9481 gaacatcagg ataatcaggc tgacgatgaa ggtaccagcc agctcgaagg agcttgcgcc
     9541 catcagagcg atgaagcaga acacgcaacc cagtacagag cagatcaggc tgacaaagtt
     9601 gcggatgtta acgccttcaa aacgaatcag gtcaacgcaa gagtagaagt acggcagcat
     9661 agtcagcagt actgcgatac cggtcagttc accgaacagg tcagatgctt taccaccggc
     9721 agagttcatc agagtgatga ggatcatcag ggcagtcatt ttcactgcag ccagcagcag
     9781 accttttttc ggaataccgt tgctgtcgac ttcaccataa actttcggga agttaccgtc
     9841 gttagcggca cgcacacctg cctggccgac cagcatcatc caggagccca gagaagtcag
     9901 gcacgcaaaa gcggtgaatg cagaaaccat cggcgcagcc cagttaccca ggatagtaga
     9961 agcactgatt gcaaacggag caccggagcc tgccattaca gaagacggat acataccgga
    10021 aagaacctga gtcgcagcaa tgtaaacaat acctgctaaa ccagtcccca gcatggttgc
    10081 cagtggaaca gtacgtttcg ggtttttaac cataccagta cttacagctg cggattccac
    10141 acccacgaag gcccacaggc agagcagaat acttttaatg attgcatgac catcagtagt
    10201 acctgcggta ttccagttag ctgcataagt tgcagcatca aaccaatgcc agccaacaat
    10261 agcagtcata accacaggaa taagaaccag taccaggcca atagtggtta aacggcttac
    10321 ccaggtaccg ccgagcatat ttacaaaggt aaatacccag acgatagcaa tacaggcgat
    10381 acccgccgga acaggatcat ttaatactgg gaagaaggtg gaaagataag atacagcggt
    10441 aataccaatt gccaggttac caatccagtt agcatggtaa taaagaacac ctgtctgaaa
    10501 accaaatgca ggagaaattt ctccggcata cgcaattggg ccaccttgtt gcgggttttt
    10561 tgttgccagt cgggcatata catatgccag cgacattgca ccaataatag agataatcca
    10621 accccagata gcaataccac cgatacttgc taggttcgca ggtaataatg caataccgct
    10681 ccccatcata ttaccggcaa caacaccggt acaggcaaat agcccgatct tcttggcaga
    10741 actcatgctc ttctcctaat ttcatttttg aatttggagt ccgggtcatg atgtataact
    10801 atttcctgac cagaccaaac tggcgctaag attactcacg aaaaaaggat taatcctaaa
    10861 gattaggtga aataacacaa aagtttctgt aagtgagaac ttgaggtttt ttattaacac
    10921 atcaggatcg caagttgata tcatgaaaag ataaacattc aatgtttaca atgtgttatg
    10981 tgacattttc tggttaaatt tatgtaataa aaattatgcg gcaaataaat tgccgcaaca
    11041 tattatacca acaggaacat acaaaaactc aacaacaaat attcccgagc atgaatcaac
    11101 ccgggagtta cttattctga agcgagaaat ttgtcgagat aaggtacaac ataaggaaca
    11161 gaagtctgga atataccatt ttcaatccag taaagggtgt ttgcccctgg gcgtaaatta
    11221 aaggcggtga gatatgcatc agctgcttcc cggttcatcc ccttcatttc ataaaccttg
    11281 ccaagcaaca cataatttag ccaggacatt tcaagatcaa tgccagtatt tatcgcctga
    11341 taagactcat ctgttttacc ttttaccagg gcactgaccg cttttatttg atatataatg
    11401 gacaggttgt tcaattccgg cagtgtaaca atgttatcta tttctgtgtt aagtgctgct
    11461 aattgttttt cgtctaaagg atgttgagaa tggcgcacga tatcaactaa tgctttttct
    11521 gctctcgcgt aggtaaattc tggggatgat tgaacaatct cacctaataa ttcactggca
    11581 cgatccaatg atttatcatc accatgcagt aaataatcat gtgcctgata aaaattagtt
    11641 aataacgcac cacgatgcgg caaaatttgc tggagcatct cctgcattcg ttgtggccac
    11701 ggttggttta acgcttttga taaactctcc agtaaatcat tttgaatcgc cagctgatta
    11761 ccgttagtga tgacataacg tttatccagc atggttgaac catctgcatt gtctaccaat
    11821 tttaccgaca taaagcattg ttgagcacgg tattggcgct gattaacaaa cgcaatagat
    11881 aatgttttac cggaactgct cggttcatca atgttgtagt tgattttgtc atgcaccata
    11941 aaggtggaga aggtgttaag tgatgtcgcc accaaatcac ccacgcctat cgcgtaagag
    12001 agctgatacg gggaactcca gctgttacaa cttttattta ccatattaat gtcaatatcg
    12061 cgtggattga gcaaaatacg cgatttgctc atcggaagac gtgtatcaag acttgaaaac
    12121 gctaccagtg ctacacagat acctaacgac aacaggaaaa aaaaccatac ccaaaaggta
    12181 gtgaatcgtt tgcttttaac tggggattgt tcaggtggcg ttgcggtgtt ttgaatgtta
    12241 agactgtggg agggagaatc tgtggcagga accgcctctg gtataggggg aggcgaagat
    12301 agcattattt cctctccctc ttcttcgctg taccagataa ccggcaccat taatttatag
    12361 ccgcgctttg gtacagtagc gatatagaca ggactatctt catcattatc ttttaatgac
    12421 ttacgtagtt ctgagatact ctgcgtcaca acgtgattgg tgacaatact tctcttccag
    12481 acattatcga taagttcatc cctgctaagt acttcgccac tgtgttgagc aaagaaaacc
    12541 agaaggtcga ttaatctcgg ctcaagggta agttgacgcc cattgcggct aatttggttt
    12601 atggacggag taacaagcca ttcgccaacg cgaactacag gttgttgcat aatagaaact
    12661 cattcgaaaa gggaatggtg caatgataat tggcataacc tatttttacc atctatagat
    12721 gggtttattt acatattatt ggtgaatgca agacgttatt tttaccagac ataaa
//
LOCUS       DAZKGF010000140        12549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZKGF010000140 DAZKGF010000000
VERSION     DAZKGF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.140"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(205..978)
                     /gene="uxuR"
                     /locus_tag="ACHOQV_003802"
     CDS             complement(205..978)
                     /gene="uxuR"
                     /locus_tag="ACHOQV_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFP9632098.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1193..2653)
                     /locus_tag="ACHOQV_003803"
     CDS             complement(1193..2653)
                     /locus_tag="ACHOQV_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFP9632099.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2734..3918)
                     /gene="uxuA"
                     /locus_tag="ACHOQV_003804"
     CDS             complement(2734..3918)
                     /gene="uxuA"
                     /locus_tag="ACHOQV_003804"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9632100.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIVGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4258..5601
                     /gene="gntP"
                     /locus_tag="ACHOQV_003805"
     CDS             4258..5601
                     /gene="gntP"
                     /locus_tag="ACHOQV_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFP9632101.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5774..6676)
                     /gene="fimH"
                     /locus_tag="ACHOQV_003806"
     CDS             complement(5774..6676)
                     /gene="fimH"
                     /locus_tag="ACHOQV_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP9632102.1"
                     /translation="MKRVIYLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6696..7199)
                     /gene="fimG"
                     /locus_tag="ACHOQV_003807"
     CDS             complement(6696..7199)
                     /gene="fimG"
                     /locus_tag="ACHOQV_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFP9632103.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTAGFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7212..7742)
                     /gene="fimF"
                     /locus_tag="ACHOQV_003808"
     CDS             complement(7212..7742)
                     /gene="fimF"
                     /locus_tag="ACHOQV_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFP9632104.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAVKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7752..10388)
                     /gene="fimD"
                     /locus_tag="ACHOQV_003809"
     CDS             complement(7752..10388)
                     /gene="fimD"
                     /locus_tag="ACHOQV_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFP9632105.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10455..11180)
                     /gene="fimC"
                     /locus_tag="ACHOQV_003810"
     CDS             complement(10455..11180)
                     /gene="fimC"
                     /locus_tag="ACHOQV_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP9632106.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11217..11756)
                     /locus_tag="ACHOQV_003811"
     CDS             complement(11217..11756)
                     /locus_tag="ACHOQV_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9632107.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     VEAGDKQMTVNMGKISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11821..12375)
                     /gene="fimA"
                     /locus_tag="ACHOQV_003812"
     CDS             complement(11821..12375)
                     /gene="fimA"
                     /locus_tag="ACHOQV_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9632108.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTA
                     IDGTHPNVLALQSSAAGSATKVGVQILDRTGAALALDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         2995 a         3295 c         3046 g         3213 t
ORIGIN      
        1 agtcattaaa cataacggaa atacgttaac atattctgat tattccgaat aatttctcag
       61 cgttaaactg atatataatt attccaaaac aatatatcgt acgtaatgat taatttacgc
      121 ataatatatt ctgcagaaca taattaacta ttcagggaac atacatagtg caaagcggtt
      181 caactttgca ccagaaatga gggatcaggc gtcgacttta tccagcggcc atgaatcaaa
      241 cagatagccg tcaaaataaa tatcgtcaac gttcgagaat tccagcagac gttgtttaac
      301 gttttccaga tgctgccaca ttgccagctt cgccgcacgg gcatcttttt tgattaacgc
      361 ggcgaggatc tgtttgtgat cgcccaacca ctctttgcga tacaggctgt catcgagatg
      421 gctgtgcaat tggatccaca ttggattgtt ttcccgccac tgccaggact gacggaataa
      481 ctccaccagc atgctgttat gcgttgcttc ggcaatggcg agatggaact gcatatcacc
      541 gctttcgctg ctacccggcg cgctggaagc cagttcacgc tcttccagtt gcaatgcctg
      601 acgcattttg acgatatctt cgcgggtagc ctgcaaagcg gcaaattccg cgatgttact
      661 ttccagtaac tgacgcgcct gtaacagctc aaaaggaccg gcatcattac agacgttggc
      721 atccggactg tctgtgttgt ggctgcctga gctatcaaga acataaatac cggcaccccg
      781 gcgcacttcc accagccctt tgatctccag catgatcagc gcttcacgta ccaccgtccg
      841 cgtgacatca agcatttctg caatttcacg ctccggtggc agccgttcgc caggattgta
      901 cggcgtcttt atgatcagat cgcggatcat cgccccgact tcctggtaag gtctttgcgc
      961 agaggtggca gatttcatgg tgttgtgtcc gttaggtgcg gttaatcaaa ttttttccgg
     1021 atgcggcgtg aacgccttat ccgacctaca acgggcaatt ttacggcacc cgttaagctc
     1081 aaactatacc attagcactg gatgttggca cgtacaggtg ggcaaaaaaa cgaaaactgg
     1141 tcaaccaaat attagtttga ttgaccagta agtctgtagg gtaatcagtt agttacagcg
     1201 cagccacaca ctcgcgcgca ccgcgctcac acagctgctg atatgccgcc gtcaccgcgc
     1261 caacaaagtc ggcgttctgc ggcagatcat cggcaaagat accgctcagg cccagcagtg
     1321 ctttcacgcg gtctgcaccc tgatactgcg cgttgatctt ctggaactcc gccagcatcg
     1381 ggtcaaccac gtcaatggca ttaccctgct catccacgcc ctgggtgtaa cgcatccagc
     1441 ccgccacgcc cagcgccagg tgacgccagc taccgccgtt ttgcaggtgc agacgcaccg
     1501 ggtccagcag acgctgcggt aacttctggc tgccgtccat tgcaatctgc caggtacggt
     1561 gacgcagaga cgggttgctg aaacgctcga tcagcagcgt cgcataagcg ttcaggtctg
     1621 taccttccgg catcgacagc gttggcgctt gttcctgcat catcagggca aaggccgctt
     1681 tgcgataagc cgggttagtc atggtgtcgg caatagtttc atagccgccg aggtaaccga
     1741 ggtacgccag gaaagagtgg ctaccgttca gcatacgcag cttcatcatt tcgaacggta
     1801 caacatccgc aacaaactgc gcgccgactt tatcccagtc cggacgacca ttaacgaagt
     1861 tatcttcaat tacccactga cggaacggtt cgcaggcaat ggcgcacggg tcgtaaacac
     1921 ccagctggtc agcaatttcc tgtaaggttt ctggcgtcgc cgccggaacg atgcggtcaa
     1981 ccatggtgca cgggaaggtg acgttctctt caatccatgc cgccagttgc gggtcacgcg
     2041 cctgagccag cccaagaacg gcaaccttcg ccacatgacc gttttcacgc acgttatcgc
     2101 aggacatcac cgtaaatgct ttcagccctt tttcacgacg cagacgcagg gcttctacga
     2161 tgtaaccaat cgcagacttc ggcgcagtcg ggttttccag atcgtgcttg atcagcgggt
     2221 tattgagatc cagttgaccg cttgccgcat ctgcgcagta gcctttttcc gtgaccgtca
     2281 gagagacaat cgccgtctgc ggacgcgcca tcgcgttgag aataccttcg caaccgtcga
     2341 tttccgggtg cagggcttct ttcatcgaac cgataatttt cagctcggtg ctttctgcgc
     2401 ctttttccgc cacggtgtac agcagttgct gttttttcag gttttcgatc agcacgcggt
     2461 cgttgcctgg catcaggtta acttcgcaga tcccccagtc gctatccgtg ctttccagca
     2521 ggtgatgagt gtacagcgcc tggtgcgcgc ggtgaaacgc cccgcaaccg agatgcacaa
     2581 tgcgtgattc cagacgagaa tgatcccatg acgggcgggc aaccggcaga ttgctgtcaa
     2641 caatagtagt catcgtaaac tccagaatcc gtcttcagag agtgacggca aggaagaagg
     2701 aaccggcacg gcgcgcagcc atgccggtgg atattaacgg ctaaagaaag cgcgctggat
     2761 cgccagttcg acaccgcgaa cttcggccag gcctttcaga cgaccaattg cggagtaacc
     2821 aggattggtt ttcttcttca ggtcgtccag catctgatga ccgtggtccg gacgcatcgg
     2881 gatcaggtct tctttgcctt ccgctttacg acggtgttct tcttcaacaa tcgctttcac
     2941 cacttcgtac atatcaacgt caccgttcag gtgcgccgct tcgtggaagg ttttcgggtt
     3001 atcttcacgc atggtggagc gcagatgggt gaagtaaata cgcggaccga actgcttgat
     3061 catatcaacc agatcgttgt cagcacgcac gccgtaggaa ccggtgcaca tggtgaaacc
     3121 gttcgccatg ctgtttacgg tatcaaccat ccactgcata tcttcaatgg tggaaacaat
     3181 gcgcggcagg ccgaggatcg ggcgcggcgg atcgtccggg tgaaccgcca tacgcacgcc
     3241 aacttcttca gcaactggaa taatcgcttt caggaagaca gcgaagtttt cgcgcagctt
     3301 cgctttgtcg atatctttgt acagctccag gtgcttacgg aactggtcga gggtataccc
     3361 ttcttccgca cctggcagac cgacaatgat gttacgggtc agacgcgctt tgtcttcgtc
     3421 gctcatagtg gcgaagcgaa cagctgcctg agcaatttct tcttcggtgt aatccgcttc
     3481 cgcgcctgga cgcttcagga tatgcatttc gaatgcagcg aattcgatct ggtcgaagcg
     3541 cagagctttg gagccgtctg gcagcacgta ttcgaggtca gtacgggtcc agtcgagcac
     3601 cggcatgaag ttgtagcaca cggtgcgaat gccgcactgc gccaggttgc gcagggtctg
     3661 ctgatagtta gcaatccact gctcatagtt gccagtgtgg gttttgatat cttcgtgaat
     3721 tggtacgctt tcaacgacag accacaccag gcctgcgtct tcaacgatcg ccttgcgttt
     3781 gaggatctct tctacggacc atacttcgcc gttcgggata tggtgcagcg cggtaaccac
     3841 gccagttgcg cccgcctgac ggacatcagc taaagaaacc ggatcgtttg ggccgtacca
     3901 gcgccaggtc tgttccatga tttttcctct ttcacatagt gggttccaca gcaaagagac
     3961 gtggagcccc ggagttgttt aggtatccgg atgaacaacc agaccgcagc cgtggcgttg
     4021 catcggatga gatggcgtat aagttctacc ttttggtaag acatgtcaac ccgaaatctg
     4081 aaattggtta accacatcac aagaatttca ctctcctggt tgttgctgca aattgcagca
     4141 aagggtaatc gatggttatt gatcagagga tgtgacattc atcgcaacaa tggttgacca
     4201 atttacataa catgtcgccc aaaataacaa cggttcaacc aggtaaggta aaacgttatg
     4261 catgtgctta acattctctg ggtggtattc ggcattggtc tgatgctggt actgaatttg
     4321 aagttcaaaa tcaattcaat ggtggctttg ttggtggcgg cgctgtccgt cgggatgctg
     4381 gcgggcatgg atttgatgtc gctgctgcac accatgaaag cgggcttcgg caacacgctg
     4441 ggggaactgg ctatcatcgt ggtgttcggt gcggtcatcg gtaaattgat ggtcgactcc
     4501 ggcgcggctc accagatagc acatacgctg ctggcgcgtc tcggtctgcg ctatgtacag
     4561 ctgtcggtga ttatcatcgg cctgattttc ggtctggcga tgttttatga agtggccttt
     4621 atcatgttag cgccgctggt tattgttatt gccgccgaag ctaaaattcc gttcctgaaa
     4681 ctggcgatcc cggcagtagc agctgccact accgcacatt cactgttccc accgcagccg
     4741 ggtccggtgg cactggtgaa tgcttatggc gcggatatgg ggatggttta tatctatggc
     4801 gtactggtga cgatcccaag tgtcatctgc gcaggtctga tcctgccgaa gttcctcggc
     4861 aatcttgagc gcccaacgcc atcattcctg aaagcagatc aaccggtaga tatgaataat
     4921 ctgccctctt tcggcgtttc gattctggtg ccgctgatcc cggcgatcat tatgatctct
     4981 accaccatcg ccaatatctg gctggtaaaa gatacccctg cctgggaagt ggttaacttt
     5041 atcggttcct cgccgattgc aatgtttatt gcgatggtgg ttgcattcgt actctttggc
     5101 accgcgcgtg gtcatgacat gcagtgggtg atgaacgctt ttgaaagcgc ggtgaaaagc
     5161 attgcaatgg tgattctgat catcggtgcg ggtggcgtgc tgaagcagac catcatcgac
     5221 accggcattg gcgacaccat cggcatgttg atgtcccacg gcaatatctc gccctacatc
     5281 atggcatggc tgatcaccgt actgattcgt ctggcgacgg gtcagggtgt cgtttcggcg
     5341 atgaccgctg ccgggattat cagtgctgca atccttgatc cagcaaccgg tcagctggtt
     5401 ggcgtgaatc cggcgctgct ggtgctggcg acggctgcgg gttccaacac cctcacgcac
     5461 attaacgatg catctttctg gttgttcaaa ggttactttg acctgtcagt aaaagacacg
     5521 ttgaaaacct ggggtctgct ggagctggtc aactccgtgg ttgggctgat tattgtgttg
     5581 attattagca tggtagcgta aaagaacacc ggagcctgcc aatggcaggc tctgttcgga
     5641 ttgtcggtaa agtgcgcttt gtttatgcca gatgcgacgc tgacgcgtct tatctggcat
     5701 acaaagggct aacgtgcagg ttttagcttc aggtaatatt gcgtacctgc attagcaatg
     5761 ccctgtgatt tctttattga taaacaaaag taacgccaat aatcgattgc acattccctg
     5821 cagtcacctg ccctccggta cgtgcgtaat ttgccgttaa tcccagactt accgccgaag
     5881 tccccactgc tcctaacgat accgtgttat tcgctggaat aatcgtaccg ttgcgcgtca
     5941 actgtacgcc gacgccctgt gctggtgaaa acgaggcggt attggtgaaa atcgagttgc
     6001 ccgcatctgc ggttgtgccg gagaggtaat accccaggtt ttggcttttc gcacaataaa
     6061 cggtaagagg aatcggcacc gaaccgggat agtccggcag agtaacggtg acatcacgag
     6121 cagaaacatc acagccgcca gtaggcacca ccacatcatt attggcgtaa atattccaca
     6181 caaactggaa atcatcgctg ttatagttgt tggtctgtcg caaaattagc acggcaatta
     6241 atgagccagc tttaatcgcc accccgcccg cactgctcac aggcgtcaaa taaagcgcca
     6301 ccggccacgg cttatccgtt ctcgaattat aaacaacgcg cggcgtttcg ctagtggtcg
     6361 gaaatggata gctactgcca ctatatttta cggtcccgga aaaattagat aacacgccgc
     6421 cataagccga gcctcgttgc agtgtgacat agtctgtaat ggtttccgga taatcgttat
     6481 ggcaaaagat ttgcgtcgaa agatccacga ccaggttttg ccccacattc acggcaggcg
     6541 caaggtttac ataaacatta gcgctgccac cgccaatagg gatagcggtg ccattggcgg
     6601 ttttacaggc gaatgaccag gcatttaccg accagcccat cagcagtaca gcaaacaggt
     6661 aaataactcg tttcattaca atcatctctt cggcttcagc tgtaggtata ggtgatgcta
     6721 attactgcct gaatggttcc ctgagtggct ccgccattta ccgtcaatgc tctgacctgt
     6781 aacgggaagt gcgctgattg tgaggaatca tccacctgaa ctgttttggt tgcgccacta
     6841 ttcaatgtgt tgccactgtc atcctgtagc tctaactgga tgttttgcgc ggtcccctgg
     6901 tttttataat atccggtact gtcggctgcc ccgctgaagc cggcggtgac cctcgacgtt
     6961 cccaccggac aattagtcaa ctcaagcgca acatcatgcc aggccgatgc cgccccggca
     7021 gacatcagac tgaaagaata aagatcgccg agatctaccg tggcattggt ggtggaaacc
     7081 gtgcacggtt tggcgacgac cttaccgttc accgtgatgg tgacatcggc tgcctgtatc
     7141 gtcgcacttg cgagcgccaa cattgccgcc aataaatacc cacgtttgcg ccatttcatg
     7201 agcatctcca gttactgata ttcaagagtg aaggtagccg tggcattgat atgccccgca
     7261 gtgacaggca cctgtgtcgc cattagccgg gcgtaaaaat tcagcgtatt tggtttaccc
     7321 ggcgtcaggg tcgtccacga aatcgcggac gatggggcat taaggggtat ctgattttgt
     7381 tgctcattca gaagctgtat tcccagtccc gcagccgctg acaccgtatt ttcaagtgca
     7441 agcaggttgg cattgtggct atctgcaaca ccggtaaacc caacttttac ggcagaaacg
     7501 gcattaccac agggtgacag caaaatacga aatggaacga caggagtcgt cgcgccaatg
     7561 ttgttaaatt gcttcaccgc gttttccatc agatcaacag taaaattggt tgattcagcg
     7621 gccacactac agccattatc tctgacatag ccgcggatag taatcgtgct atccgcagcc
     7681 aaagcgtgac ttaccgccag ccacaaaaaa gcgcaaagaa gataaaaagg tttgtttctc
     7741 atcacgcctc cttaacgaca ttcagctgat agctgggtta ataactgctg ctgactctct
     7801 ggtggcagtt gataattggc gacacagtga gcattttcct cttctcccca tttcacctga
     7861 acttttcccg ctaaaggcat tccgctgagg taaacctgac cattatccgc aacaatgccg
     7921 ctactctggc tactctctga tgtcaccatc gccccaaacg gcagcggctt attattgtgg
     7981 gttagcgtca tgagcagttt tatcccaacg cgcgctttaa actctgctcg cacgatcgcc
     8041 ccacgggtgg gaacaacgtt agcgaccgcg ttatctaaat cgacgttatc agccagggta
     8101 ttggtatcca gcgctactct gttttcccga tattcagtgg cataaggcag cacggcataa
     8161 ccgcgccagt cggtacgcac ccccgtctgg ttttcgactt ttgcatcttt tgcgccaggc
     8221 gctttaacaa gcaccaccgt atcgtttaac ggctgcccca gcgttacgcc attggcatga
     8281 gccagtaccc caccgctgac tccgtaatag agctgcttaa tatcatcgct atggctgtaa
     8341 ccgatgttgg cattgccgta accaccgcga taattcagcg tggcgtagcc tgtacttccg
     8401 ctattaccat cacctccccc ggcgtaaccg gtttgcacgc tatagctgag gttgttgtct
     8461 tccagcaacg taccgtatac accggccaga ttggtcatcc gaccgttgag atcgtgtgac
     8521 atgctgtaac tggcactggc atgtcgccac tgagatttac tgtcagaacg cagccagtgg
     8581 ctgaaaggaa tattgacgtt aagcgctaac atctgatccc gtcctttttg ccaggcgttt
     8641 ttcgtcaggc tatagctgag cgtccagttg atatcttcga atgcagtatt taaaccagcc
     8701 tggaattgct catcgacatt actcgttccc caataagttt gatggctacc actcaaatac
     8761 agtgttgatg agcgcccgag ttgctgagta acggtgagct gtaatttccc gcgtttgtta
     8821 taagcgagat tgtaatagtc ggtgaatttc ggcttaacct gaataactcc atcctgtgtt
     8881 tcgatgttgt agccattcat tcgactgtat gttgtatcag cgaaattaaa atacccactg
     8941 gtcgaataac ggtaacccac taactgaata ttcgtgcctg actcattgag cgatttgtta
     9001 taaagaaaac gcaccgattg tccgtcatgc tgactgtcat cgggaagtgt ggaattagcc
     9061 tgcgtcatat ccacagacag agcgcccagt gcccccatat ttttcccgat accgaaatta
     9121 aacgcacgat aacgatccgc cagttgcgtt ccaccatata ttgtccagcc agccggaagg
     9181 ccgtggagta atgtactctg gaaaaagcgg ggtttttcct gttgcgcatt tccactacgg
     9241 tattctcctg ccgtaatgga ataacgtgta tgcccttcac gctgcaaaag cgggactgac
     9301 gaatagggta cggtaaaaat ctgcgtactg ccgtcagcct ctttaatcgt tacctgcaag
     9361 tcaccactat tacctgcggc atagatatcg ttgatggtaa aaggccccgg tggcaccgta
     9421 ctattataaa tgtcataccc attttgttta atagtgacct gtgcagtacc acgagcaata
     9481 ccgtggatca ccggggcaaa tcctctttgg ctatcgggta acatattgtc atctgaggcc
     9541 aattgtgcgc cgcgaaagtt aataccatcg aaaatatcgc cctgagtata accatcaccc
     9601 agcgtcagcc gggaacgtaa cggaattatg tctcgctcaa gccaggtatt gatatgctgc
     9661 catttatttt tgctacctga tgaactgtcg ctactgttat aactccaggt ggtattgtcg
     9721 cgtaaacgcc acgcaccaat atttaaccca ctctgtaggt ttaaatatgc ataatggctg
     9781 ttacccccaa ttcgattctg tacactattt ccgctgaaat tataattgag caatccggca
     9841 ttaataccgg gatcccataa ctcaggagga atataaccac gcgcgcgatt actcataaat
     9901 gcctgaggga tcgtcaggtt gagtcgctgc tgaccaacat ctaaatgcgc agtagcgtca
     9961 tggatcattg aggttaatgg cacgcaggca tcatccgcca gcagattcat accggagaca
    10021 gaagccgtat tcagccccat actggcaagt tgcgcgcgtg tcaggcaggg aacaatccct
    10081 tgttcactgt cgcccgtgtt aaatgtgaca tcacgcgttg ccatataacc attattcaaa
    10141 tagatatcga cgcgatacgt ccctggcggt aattcttgcc cattttcaaa acgcgataaa
    10201 tcggccacag cctggggatc atccgctaaa aagcgcggat taaaatagag ttcggcagat
    10261 gacaaaggtg cctgtgcggc aaaagcacag gcgacaaaga gccggacaaa aaaaccagcc
    10321 aaacgatgct tacgaatatg caagcattgt gtgtttcgct ggtaaagtct taaattcaga
    10381 tatgacatta ttcctccggt ttaaaatcac ccggcagtca attcttttta ttcaggcgaa
    10441 aaattccccc tgcgttattc cattacgccc gtcattttgg gggtaagtgc gccataatca
    10501 tttattgttc ggtaagtaat attgcttcct gcatcagaag gcaatttaac cgtgctttcg
    10561 cccattggag gcaccaatgc attttcaaga acccgggttc cggcattcaa ctctgttacc
    10621 gtcaggtaat agggtgtcgg gttaatcagc gtcagagaat tcgcgctacg acgaaatctt
    10681 aatttttctg cggcctgatc gggtggcaac gctaatttag ccgggcgata gtacagctta
    10741 atgcggctga taattgcgag ctgtagcgta ttctcagtca attttgattt atccattgac
    10801 ggaatcgctt taacgttcat ccagaataaa ctttcccgat cctgtggcaa ttggttattt
    10861 gttgcatcaa gaatacgtaa ggtattctct ctttttccct tcatcgcaaa cagaggtggc
    10921 gtcacgataa aacgaccatc ctttacacca tcggcatttt ccacccatga ttgaattaaa
    10981 taggtgctat tttcatcatt atttgtcacg gcaagttgca cttgtttttg ccctgccgga
    11041 taaattacgc gggtcgcacc taaggccact cccgcttcag cgcgtccggc aaccatcatt
    11101 gccatgaaca tcaggatacc tgccagcaag cagaatgtta tttcctgcga tttccttaca
    11161 ttgacgtttt tattactcac tgtcctgttc ctgttatcac attatctgct gaacaattac
    11221 tgataggtta aagagaacca ggcctgggca ttggcgatgc cgccagtaac ccgacgcccg
    11281 gtagcacgat atttggcgat gaaatgtagc gaagtagagc ctgagtaaag ccgtttccag
    11341 tttgctggag gacgattaat cggtacgagg tttccttcat catcaaataa cgctacgcca
    11401 atattggtgg ctatccctgg cccctctccc acggaaagca catccggatt tttaccatcc
    11461 gcgacaccgt gaaacgccac acccacacgt tcactcacca ccgtgctaca ttcccgtaaa
    11521 tgaataacaa aaggcaccgg tgcgctatct tcccctaccg catgaaaccg gttactgctg
    11581 attttcccca tattgaccgt catttgttta tcaccagctt caactcggca agtttccgca
    11641 ataatgacgc cctgaaattg catatttccg ccgggcagcg tggtattcca tttatttccg
    11701 gccagagcaa acattggcag caacgacgct aatagaaata atctcttcct tttcatcgtt
    11761 atttttatcg cacaagggtg ggcatccctg cccgtaatga cgtccctgaa cctgggtagg
    11821 ttattgatac tgaaccttga aggtcgcatc cgcattagca gcacccgggg ttgcctcgcc
    11881 gattgcataa taacgcgcct ggaacggaat ggtgttggta ccgttattca aggttgtttg
    11941 tgcactaaat gtcgcaccgt ccagcgccag cgcagcaccc gttctgtcca ggatctgcac
    12001 accaactttt gttgcgctac ccgcagctga actctgcaaa gctaatacgt taggatgagt
    12061 tccatcaatt gcggtaccaa ggaaggcaat agcagctttg tctgcaacag tagtgtcaca
    12121 atcgttcagt tgaatgttaa aaccgacagc agaactggtt gctccttctt gtgccagcga
    12181 tgctgtacga acctgcccta actgaacagt ttgatcaaca gagcctgcat caactgcgca
    12241 agcggcgtta acaacctccc ctttaaagtg aacggtccca ccatttaccg tcgtgggtgt
    12301 agtttcagcc agagccgctg tagaactcag ggacagagcc gacagaacaa caattgccag
    12361 agttttaatt ttcatactgc tttcctttca aaaaactatt tctaaatcga catgggcagt
    12421 cgttctgtac actttgtttt gtcaacgagt ttgactgcca acactgcaca gttttccccc
    12481 aaaagatgaa acatttgggg ccattttgac tcatagagga aagcatcgcg cacaaacttt
    12541 ttcagttta
//
LOCUS       DAZKGF010000141        12428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZKGF010000141 DAZKGF010000000
VERSION     DAZKGF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.141"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            156..1049
                     /locus_tag="ACHOQV_003813"
     CDS             156..1049
                     /locus_tag="ACHOQV_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9632109.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAIKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIESGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(1080..2069)
                     /locus_tag="ACHOQV_003814"
     CDS             complement(1080..2069)
                     /locus_tag="ACHOQV_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9632110.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQSIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQISEALETIKIVGERYSPEHQARVGR"
     gene            complement(2096..2947)
                     /locus_tag="ACHOQV_003815"
     CDS             complement(2096..2947)
                     /locus_tag="ACHOQV_003815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9632111.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            3513..7763
                     /gene="fdeC"
                     /locus_tag="ACHOQV_003816"
     CDS             3513..7763
                     /gene="fdeC"
                     /locus_tag="ACHOQV_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFP9632112.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAQPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGITVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGELEIEALVKNSQKTVKVKF
                     KADFSTGQASLEVDAAAQKVANGKDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPAKLVLDPNGTATTNEQGQA
                     IFTATTTVAATYTLTAQVSQNNGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(7888..8745)
                     /locus_tag="ACHOQV_003817"
     CDS             complement(7888..8745)
                     /locus_tag="ACHOQV_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9632113.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKSFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARMNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            8994..9863
                     /locus_tag="ACHOQV_003818"
     CDS             8994..9863
                     /locus_tag="ACHOQV_003818"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9632114.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(10023..10616)
                     /gene="rclC"
                     /locus_tag="ACHOQV_003819"
     CDS             complement(10023..10616)
                     /gene="rclC"
                     /locus_tag="ACHOQV_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFP9632115.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIIFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQHLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(10628..10864)
                     /gene="rclB"
                     /locus_tag="ACHOQV_003820"
     CDS             complement(10628..10864)
                     /gene="rclB"
                     /locus_tag="ACHOQV_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFP9632116.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     NAIADMARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(10973..12298)
                     /gene="rclA"
                     /locus_tag="ACHOQV_003821"
     CDS             complement(10973..12298)
                     /gene="rclA"
                     /locus_tag="ACHOQV_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFP9632117.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADIPNIDVIDGQAEFI
                     NNHSLRVHRPGGNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         3324 a         2820 c         3074 g         3210 t
ORIGIN      
        1 agggtacgtt tattcataat aaatctcctg aaagtttaga attattttcg atacagccag
       61 attatcattg ttactaataa tgtggttaaa ataatagcat ctatgccccc ttcatgagtg
      121 tgtgtgaaga ggatgcttct acaaggaaat cgccaatgct caaagaaaac ttcaacgaac
      181 tgcaaatctt tcttgtggtg gcaagagagc gaagttttac caaagcagcg ggcaaactcg
      241 gcgtctctca gtctgcactt agccatgcaa taaaggctct ggaggaaagg ctcaatatcc
      301 gcctcctgac acggactacc cgcagtgtgg ctcccacaga agcgggggag agaatcattg
      361 cctgccttga accgcgtatt gacgagcttg aacaggaact ggaatcactt attcaattga
      421 acggcacgcc ctccgggaat attcggttat ctgccgggga acatgccgcg cgaagtctgg
      481 tctggccgaa gctaaaacct tttctcagag agtatcccga aatcaatgtc gaactggtgg
      541 tggataacgg ttttgtcgat attgttgaag gccgttttga tgccgggata cgactgggtg
      601 aaaatgttga taaagatatg gttgcagtca ggattggacc agatatgcgg atggctgtgg
      661 tgggagctcc ggcgtatttt gctgcaaacc ctgcgcctga aacgcctcac gagctacaaa
      721 atcatcgctg catcaacatg cgtttgccaa ctgccggtgg gctttaccac tgggagttcg
      781 agcgagaagg gaaaccgctt cgcgttaagg ttgatgggca actgacgtgt agtccattgc
      841 cagaaagaat tgatgccgcg ttatcagggt ttgggatcgc ttgtgtccct gaagacatgg
      901 ttcaggagta tatagagtca ggcaagctta ttcaggtgtt acaggagtgg tgtccaactt
      961 ttcctgggta ttatctttac taccccagcc gcaagcagca tccgccagct tttgcgctgt
     1021 tgatcgatgc gcttcgctac accgaataac gggtccttta tcctggaccc gtttgggtat
     1081 tagcggccta cgcgtgcctg atgttcaggc gagtaacgtt caccgacgat tttaattgtt
     1141 tccagcgcct cgcttatctg ctgcgtatct ttctgcgaaa gaacaatgtc agcagcggcc
     1201 aggttttctt ccagccggtg cagtttagtg gtgccaggga tgggaacaat ccacggcttt
     1261 tgtgctaata gccatgccag tgcgatttgt gcagacgtta cccctttctc cgcagccagt
     1321 tcacctaaca aggtgaccag tttttcattg gcttcaatgg attgtgctgc gaaacgcggt
     1381 acggtgctgc ggtaatcatc tttgccgaaa gtggttcccg gcttaatcgc ccctgtcagg
     1441 aaacctttac ctagtgggct gaaggggaca aaaccaattc ccagttcctc cagcaacggc
     1501 agaatttcct gttcaggctc acgccaccac atggaatatt cgctttgcag ggcggtgaca
     1561 ggttgtaccg catgtgcacg acgaatggtt tgtgctcctg cttcagacag gccgaaatgt
     1621 ttgaccttac cttctgcgat gaggtctttc accgttcctg caacatcttc aataggaaca
     1681 tctggatcaa cacggtgttg atacagcagg tcaataacat ctgttttcag acgacgtaat
     1741 gatccttcaa cggcttcacg gatatgttcc ggacggctgt ttaaaatctg ctgcttatta
     1801 tcatcgccga aggtaaaacc gaatttagtg gcaattacca cgcggtcacg aaacggtttt
     1861 aaggcttcgc cgaccacttc ttcattcaga tacggaccat acacttcggc ggtatcgaag
     1921 aaggtgacgc cacgttcaac cgcagcacga ataagttcga tagcctgacg ggtatcggtc
     1981 gccgggccgt agccgtggct taagcccatg caaccgagcc caagggcgga gacttcgagt
     2041 ccggatttac ccagataacg tttttgcatt gaatgaatac ctcttttatc gcggcttaaa
     2101 catcaagttt gcggccagtc agccattcca ccattgcggg atcgcggtga gagaagaacg
     2161 cgctggttgc ggtatccagg gcggtaattt gcagcatatc ttcagggctg agttcaaaat
     2221 cgagaatgtt gatgttctct tccatgcgtt cttttcgcac tgattttgcc agtgaaacaa
     2281 tgcctcgttg gtagatccag cgcagaacaa cctggcccac gcttttgcca tacttctcgc
     2341 caattgccgt gagaacagga tgctggaaca gaccattttt cccttccgca aacggtgccc
     2401 aggcttctgg ctgaatgcca cgactttgca tccagggaac ggcctgtagc tgctgattga
     2461 aagggttaac ttcaacctgg tttaccgccg gaacgacgtt attaaaagcg ataaggtctg
     2521 ccagtcggtc aggatggaag ttactgacgc caatagcgcg aattttaccg gcctgttgca
     2581 gctcttccat ggcacgccag gctccgtgga catcgccata tggctggtga atcaagtaca
     2641 ggtcaacgta atcaagttgc agtcgattca gagagcgttc gaactgggct ttggcccctt
     2701 cgtaactggt atcctgcaac cagagtttcg tggtgacaaa gagttcgtta cgggcgatac
     2761 cgctttgttt cagtgcgttc ccgacctgga tttcattttg ataagaggcg gcggtgtcaa
     2821 tcagtcgata accgctgtta atagcgtcaa tgaccgctct ttcgcattcg gcggcatcgg
     2881 tcatctgaaa tacaccaaag cccaacaggg gcatttcaat accgttgttc agttttactt
     2941 tttgcatggt gttatccttc cgttatcgtt catgagaagc ataacgtaaa gggaagagct
     3001 cgattagacg gcaggatttg tcaggggtta tgaacaaaat tcataaatct gtttgagggt
     3061 tgagattagt cgtgcaagtt cgatatcccc ggaatttaca gtccgatgaa taaaaatgaa
     3121 tgtccataaa aaatatattg gtttatccca gcgtatatac ttacttcgac gtactcccaa
     3181 tagcagttaa ttaaagacgg gtcattatta ttcactctga ataatgaatt attcactgcc
     3241 cgcagagtaa gaaatataac ttaagtatct atttaatgac ttgcacaaaa agctaaattt
     3301 ttacccataa ataaaaatat aatcccgcgc ccaaccacct gatgagtggc tacagacact
     3361 ggatatatta ggtggcggtg tactttctta cataaaggta tttccttttc tgtggaaaag
     3421 gaaaccggga aatccccggt ttttctgaca agcagacgcc attatttgtg tctgcctatg
     3481 ttcgttaatt cgttcatcag gaaattatct caatgtcacg ttataaaaca gacaataaac
     3541 agccacgatt tcgttattca gttctggccc gctgcgtggc gtgggcaaat atctctgttc
     3601 aggttctttt tccactcgca gtcaccttta cgccagtaat ggcagcacgt gcgcagcatg
     3661 cggttcagcc acggttgagc atggaaaata ctacggtaac tgctgataat aacgtggaga
     3721 aaaatgtcgc gtcgcttgcc gctaatgccg ggacattttt aagcagtcag ccagatagcg
     3781 atgcgacacg taactttatt accggaatgg ccaccgctaa agctaaccag gaaattcagg
     3841 agtggctcgg gaaatacggt actgcgcgcg tcaaactgaa tgtcgataaa aatttctcgc
     3901 tgaaggactc ttcgctggaa atgctttatc cgatttatga tacaccgaca aatatgttgt
     3961 tcactcaggg ggcaatacat cgtaccgacg atcgtactca gtcaaatatt ggttttggct
     4021 ggcgtcattt ttcagaaaat gactggatgg cgggggtgaa tacttttatc gatcatgatt
     4081 tatcccgtag tcatacccgc attggtgttg gtgcggaata ctggcgtgat tatttgaaac
     4141 tgagcgccaa tggttatatc cgggcttctg gctggaaaaa atcgccggat gttgaggatt
     4201 atcaggaacg cccggcgaat ggctgggata ttcgtgctga gggctattta cctgcctggc
     4261 cgcagcttgg cgcaagcctg atgtatgaac agtattatgg cgatgaagtc gggctgtttg
     4321 gtaaagataa acgccagaaa gatccacatg cgattaccgc tgaagtgaat tacacgccag
     4381 tgcctcttct gaccctgagt gccgggcata agcagggcaa gagtggtgag aatgacactc
     4441 gctttggcct ggaagttaat tatcggattg gcgaacctct ggaaaaacaa ctcgatacag
     4501 acagcattcg cgagcgtcga atgctggcag gcagccgcta tgacctggtt gagcgtaata
     4561 acaatatcgt tcttgagtat cgcaaatctg aagtgatccg tattgctctg cctgagcgta
     4621 ttgaaggcaa gggcggccag acggtttccc tggggcttgt ggtcagcaaa gcaactcacg
     4681 gtctgaaaaa tgtgcaatgg gaagcgccgt ctttgctggc cgcaggcgga aaaattacgg
     4741 ggcagggcaa tcagtggcaa gtgacgctcc cggcttatca ggcaggcaaa gacaattatt
     4801 atgcgatttc agcgattgcc tacgataaca aaggcaatgc ctcgaaacgt gtgcagacag
     4861 aagtggttat tagcggagct ggtatgagcg ccgatcgtac ggcgttaacg cttgacggtc
     4921 agagccgtat tcaaatgctt gctaacggta atgagcaaaa gccgttggtg ctgtctctgc
     4981 gcgacgccga gggtcagcca gtcacgggca tgaaagatca gatcaagact gaactaacct
     5041 tcaaaccggc tggaaatatt gtgactcgta ccctgaaggc cactaaatca caggcacagc
     5101 caacactggg tgagttcacc gaaactgaag caggggtgta tcagtctgtc tttactaccg
     5161 gaacgcagtc aggtgaggca acgattactg ttagcgttga tgacatgagc aaaactgtca
     5221 ctgcagaact gcgggccacg atgatggatg tggcaaactc caccctgagc gctaacgagc
     5281 cgtcaggtga tgtggttgct gatggtcagc aagcctacac gctgacactg acagcggtgg
     5341 actccgaggg taatccggtg acgggagaag ccagccgcct gcgacttgtt ccgcaagaca
     5401 ctaatggtat aaccgttggt gccatttcgg aaataaaacc aggggtttac agcgccacgg
     5461 tttcttcgac ccgtgccgga aacgttgttg tgcgtgcctt cagcgagcag tatcagctgg
     5521 gcacattaca acaaacgctg aagtttgttg ccgggccgct tgatgcagca cattcgtcca
     5581 tcaccctgaa tcctgataaa ccggtggttg gcggtacagt tacggcaatc tggacggcaa
     5641 aagatgctaa tgacaaccct gtaactggcc tcaatccgga tgcaccgtca ttatcgggcg
     5701 cagctgctgc tggttctacg gcatcaggct ggacggataa tggcgacggg acctggactg
     5761 cgcagatttc tctcggcact acggcgggtg aattagacgt tatgccgaag ctcaatgggc
     5821 aggacgcggc agcaaatgcg gcaaaagtaa ccgtggtggc tgatgcatta tcttcaaacc
     5881 agtcgaaagt ctctgtcgca gaagatcacg taaaagccgg tgaaagcaca accgtaacgc
     5941 tggtggcgaa agatgcgcat ggcaacgcta tcagtggtct ttcgttgtcg gcaagtttga
     6001 cggggaccgc ctctgaaggg gcgaccgttt ccagttggac cgaaaaaggt gacggttcct
     6061 atgttgctac gttaactaca ggcggaaaga cgggcgagct tcgtgtcatg ccgctcttca
     6121 acggccagcc tgcagccacc gaagccgcgc agctgactgt tattgccgga gagatgtcat
     6181 cagcgaactc tacgcttgtt gcggacaata aaactccaac ggttaaaacg acgacggaac
     6241 tcaccttcac cgtgaaggat gcgtacggga atccggtcac cgggctgaag ccagatgcac
     6301 cagtgtttag tggtgccgcc agcacgggga gtgagcgtcc ttcagcagga aactggacag
     6361 agaaaggtaa tggggtctac gtgtcgacct taacgctggg atctgccgcg ggtcagttgt
     6421 ctgtgatgcc gcgagtgaac ggccaaaatg ccgttgctca gccactggtg ctgaatgttg
     6481 caggtgacgc atctaaggct gagattcgtg atatgacagt gaaggttaat aaccaactgg
     6541 ctaatggaca gtctgctaac cagataaccc tgaccgtcgt ggacagctat ggtaacccgt
     6601 tgcaggggca agaagttacg ctgactttac cgcagggtgt gactagcaag acggggaata
     6661 cagtaacaac caatgcggca ggtaaagtgg acattgagct tatgtcaacg gttgcagggg
     6721 aacttgagat cgaggccttg gtgaaaaact ctcagaagac ggtcaaggtg aaattcaagg
     6781 cggatttcag taccggtcag gcgagcctgg aggtagacgc cgctgctcaa aaagtggcaa
     6841 acggcaaaga tgcctttacg ctgacggcaa cggttaagga tcaatacggc aaccttcttc
     6901 ctggcgctgt ggtcgtcttt aatctgcctc ggggcgtcaa accgcttgca gacggtaata
     6961 tcatggtgaa cgccgacaag gagggtaaag cggaactgaa agtggtttcc gtgactgccg
     7021 gaacctatga gatcacggcg tcagcaggaa atgaccagcc ttcgaatgcg cagtctgtaa
     7081 cgtttgtggc tgataagact acggcgacca tctccagtat tgaggtgatt ggcaaccgtg
     7141 cagtggcgga cggcaaaacc aaacagacgt ataaagttac ggtgactgat gccaataaca
     7201 acctgctgaa agatagcgaa gtgacgctga ctgccagccc ggcgaagtta gttctggatc
     7261 ccaatgggac ggcgacaacg aatgagcaag ggcaggctat tttcaccgcc accacaactg
     7321 ttgcggcgac atatacactc acggcgcaag tgagtcagaa caacggtcag gtatcaacga
     7381 aaaccgccga atctaaattc gtcgcggatg ataaaaacgc ggtgctcgct gcatctccag
     7441 agcgtgtaga ttctctggtg gcggacggga agactactgc aacactgacg gttactctga
     7501 tgtcgggtgt caaccccgta ggaggaacca tgtgggtcga cattgaggct ccggaagggg
     7561 tgacagaggc ggattatcag ttcctgccgt cgaaaaatga ccatttcgcg agcgggaaaa
     7621 tcacgcgtac atttagtacc aacaagccag gtacatacac attcacattc aactctttga
     7681 catatggagg gtatgaaatg aaaccagtga ctgtgacaat taacgccgtt cctgcagata
     7741 ctgaaggcgc tgaggagaaa taaaaacgac gatgtaataa ataataatgg cgttgacatg
     7801 cgcagcgcca ttatttactg aatacctacc ctggcaccga ttagacggtg gcagggtagg
     7861 gtaaatctta tgtgatggag ttacaggcta aaccgggcga acggggataa aaatttcgca
     7921 aacaatgtta ttgctatcaa tttcgacgaa gttttcattg taataaaatc gctcaatatc
     7981 actgccgttc attctggcta accccctggc aggtaattcc atcagataga ggtttcgcgc
     8041 ccattcaggg tagtgctccc actcaccttc atagcggaaa gaggcataca gtcctcggtt
     8101 aattgtccac tctttcgaca tctctgaatc ggtttgtttg cataccgtac cggcaaaagt
     8161 ttcaactgtg agcaaatcga ccgattttgt tgatggttcg aacgtggaga cgaaatagat
     8221 atccgtctta aaactcttca tacatctggc taaatgatga cgtcgcagtt tgacttcttc
     8281 atccggtgat ttattcgaca cttcaatatc atatttaaat gaattaccaa aaataggtgt
     8341 ctcaggaaag ttgactaatc tacactccgt cttttgctgt tgacgtatta aaaaagaagg
     8401 gaagatattt gcgagatccc aataatcacg gtggcggtat tcacggggag agcagccgaa
     8461 taacttttta aattcgcggc tgaatgactg ctgtgagtca aaatgcaaag agagtgcaat
     8521 atcaagcata gatttcgcgg agagccggac aagaatggcg gcacgacaaa gccttcgttt
     8581 gcgaatgtat tctcccaaag gcacatgcat gaaatttcgg aacagaagct ggatgttgcg
     8641 tctgctgtag ccagatttct gtgcgatatc ttcgattgaa atagggtgtt cgagattgca
     8701 ctcaatccac tccaggagct gctgtagaat cttctgcctg atcatcacat tctccgttaa
     8761 ttctttgtca tatcgcacat tttcgttatg ccctgggccc taagtgtatc aaaaacagca
     8821 agggcgaagg caaaagcact ggtagcgtct gcttcgttgt ccccgaaaat agagaggttt
     8881 cagtcctaca ttattaatga attttttgca taagtgatat caaaatccac atactaattt
     8941 gaggttacgt tttaacgtag actcattgtt catgcctaat ggagggactg acagtggaat
     9001 tttccgtatt aagtaacaat ctgaaaatgc cgatgatggg atttggtgtt tttcaggtta
     9061 ccgataaaaa cgtgtgcaaa cagtcagtgt tgaacgctat ccgcacgggt tatcgactca
     9121 tcgacactgc agcggtatac ggtaatgaag atgccgtcgg tgaagccgtt cgtgaagcta
     9181 tatctgaagg tttatgtacc cgagaagagt tatttataac gtctaaattg tgggtgcagg
     9241 atatgttgaa tcaggatatt gcagcagcag gtattgaagc atcattaaaa aaatcgggac
     9301 tagaatactt cgatctctat ttattgcacc aggccatgcg cgattatttt agcgcgtggc
     9361 gtgcactcga agatgcctat gaagagggca aattaaaagc gattggggtt tccaatttct
     9421 accctcatgt tctggcgaac ttttgtgaaa cggtaagagt taaaccgatg gtcaaccagg
     9481 tcgagttgca tccctatttt gcccaaccag aggcgctggc aaccatgaag tattataacg
     9541 tgcagcctga agcatgggct ccgttaggtg gtggacgaca taaacccttt gaaaataatc
     9601 tgcttcagag tattgcagat gcccatcaaa aatcgatttc tcaggtcatt ctgcgttgga
     9661 atattcaacg gggagtggtc gttattccga aatcgacaca tcaacagcgt atcgaagaaa
     9721 attttgctat ctgggatttc tcactgacag agaaagaaat ggcgcaaatt agttcgcttg
     9781 atttgggtta tgttggggaa tcggtaaaac attttaatcc tgagtttgtt cgtggttgtc
     9841 ttgctgtaaa aatacatgat tgatattaat cacttatttt acctgagacg acaagaatct
     9901 tttaacaggg gagtgatatt gatcttcact ctgtcatatc tccggtaata tggcgtcagg
     9961 cttcatcatc agaaaggggg cctgacgcct gaaaaagtga acaacagaca gtgttcggat
    10021 tatcaatatt cagtttttaa cgttgaactg gattcattac tgcgttgttt aagaatatcc
    10081 cgcgccgaat ctgccattat catgacagca cctgccagca tcagagtatc tttcaatacc
    10141 agacgaccag caccggataa ataagggaaa ccatgatgag cgtcacccaa tgcgggtacc
    10201 catgcctccg gggtggtgat taaaaatgag agtgttacca gcggcgtggt aaatgccatc
    10261 agcccaccca ataaacctaa ccagcgattg acaggattag ccaaaaccag cagagcaata
    10321 atcacctcca cgacgccaag accgttggaa aaaccatagg tattattagc cgtttgccat
    10381 gctcttgctt ctggtttgta ttcgccttcg tgagtcagat gctgtttata gtcttccggg
    10441 tgttcataaa agaacgacat tagtggactg tttgcgacga atggtgtaat gctgtctgcc
    10501 tcgtaaggga caaactttaa taacccaatc cacataaaaa taatcgcaat actcagacga
    10561 atcaatgtca ggccaatttt atctccccga cttaacaggt gcaggtatct ttccattgta
    10621 aacatcctta tttatacaat actgctgttg cggttgaatt attatcaatt cgcatcgatg
    10681 taactttcca ggatgaagca ttatattttc tggccatatc agcaatggca ttctcaagaa
    10741 ctgtcggtga tgctgatact gatgagacgc tgatatgttt cagaattatt ttatcatctg
    10801 cattggtgga aaatgccata acgccagata atagtgttgc aaataaaaca gattttttaa
    10861 acatataaaa taccatcaat gaagtgatta agaatttata gagtaacaga gaattaataa
    10921 gattcttttc tctgagacgc caggatattt gttctggcgt ctgattttga gtttatttga
    10981 ctaatgaaaa tagatcattg agtgattcgc tcatcgacgg atgagtaaat atctgatcgc
    11041 gtaatatgct ataaggcagc ccggcatcca tcaccatttt cactatattg atcatctcgt
    11101 gggagtcaac acatagcagt gatgccccta atatacgttg ggttttatta tcaacaatcg
    11161 cttttaatac cccacgggta tcgttcatca ctctggcgcg cggaattgca gctacaggca
    11221 atgtcaccac ctgaatatca gcaccactct ctctggcttg ttcttctgtc ataccaaccc
    11281 tggacagggg cggtgtcata aatacggaat aaggcacatt tttccgatca tcagtactac
    11341 gtttgccttc acccagtaac tcatcacgta caatgcggta atcatctagt gatatgtaag
    11401 taaattgcag cccgccggta acatctccca tcgcccaaat attgtctgcg gtggtatgta
    11461 atcgcttgtc aacgacaatt gccccgcgct cgtttactgc gataccggca ttttctggat
    11521 gtaacgaagc ggtagctggt tgacgaccgg aagctattaa cagtgcatcc accgccagtt
    11581 gggcgtgctc gctatgcact tgcacttgat tttcatggtg actgattcgc tccacatggg
    11641 cattgaggat tatatcgaca ccctgatcgc gtaaaatcgt cgcgatatta tcagcaatat
    11701 cccgatcttc ccgaggcaaa aacagcgaag ctgcctctaa aatggttact ttgctgccaa
    11761 aattagcgaa catagaggcg aactcaacgc caatatatcc gccgcccaaa atacctaaat
    11821 gcccaggcaa ttcttttaga ttaagtaatc cggtgctgtc atatacacca ggcgtggtgg
    11881 taattccagg aattggcgga accacggctt gtgcaccggt attaataaaa attttctcgc
    11941 catgaatctc cagatttccc ccaggccgat gaacacgcag gctatgatta ttgataaact
    12001 ccgcctggcc gtcgatcacg tcgatattgg gtatatccgc aagattatga aaattcttat
    12061 tacgtaaaaa attaaccact tcatttttac gctgtatggc acggacaaaa tctgtgtgct
    12121 gctgtgcgtc atgaaccaat gttttggttg ggatacagcc gatattaata caggtcccgc
    12181 catacattgc atttgattgt tcgatgagag ccacacgcca acctgctttt gccagcgtga
    12241 cggctaatgt ttttccagcc ttgccaaaac caataataac tgcctgatat ttattcatga
    12301 tgatttcctg tcgaaacgcg ttcatttgac atcatgacta tagacgcaaa agagcaaata
    12361 acatttcttc acaacgcttg atattttgtc ttatcgtctg gtattttctt tttcttaatc
    12421 agaatcac
//
LOCUS       DAZKGF010000142        12318 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZKGF010000142 DAZKGF010000000
VERSION     DAZKGF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.142"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(117..422)
                     /gene="ynfD"
                     /locus_tag="ACHOQV_003822"
     CDS             complement(117..422)
                     /gene="ynfD"
                     /locus_tag="ACHOQV_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFP9632118.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            530..1240
                     /gene="ynfC"
                     /locus_tag="ACHOQV_003823"
     CDS             530..1240
                     /gene="ynfC"
                     /locus_tag="ACHOQV_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFP9632119.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAQT
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(1243..1803)
                     /gene="speG"
                     /locus_tag="ACHOQV_003824"
     CDS             complement(1243..1803)
                     /gene="speG"
                     /locus_tag="ACHOQV_003824"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFP9632120.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(1838..2179)
                     /gene="ynfB"
                     /locus_tag="ACHOQV_003825"
     CDS             complement(1838..2179)
                     /gene="ynfB"
                     /locus_tag="ACHOQV_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFP9632121.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2314..2640
                     /gene="ynfA"
                     /locus_tag="ACHOQV_003826"
     CDS             2314..2640
                     /gene="ynfA"
                     /locus_tag="ACHOQV_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFP9632122.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            2846..4060
                     /gene="rspA"
                     /locus_tag="ACHOQV_003827"
     CDS             2846..4060
                     /gene="rspA"
                     /locus_tag="ACHOQV_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFP9632123.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            4072..5091
                     /gene="rspB"
                     /locus_tag="ACHOQV_003828"
     CDS             4072..5091
                     /gene="rspB"
                     /locus_tag="ACHOQV_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFP9632124.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            5149..6516
                     /gene="ydfJ"
                     /locus_tag="ACHOQV_003829"
     CDS             5149..6516
                     /gene="ydfJ"
                     /locus_tag="ACHOQV_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFP9632125.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVTAAQ
                     PSRSL"
     gene            6606..8066
                     /gene="ydfI"
                     /locus_tag="ACHOQV_003830"
     CDS             6606..8066
                     /gene="ydfI"
                     /locus_tag="ACHOQV_003830"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9632126.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAVMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEENVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(8102..8305)
                     /gene="ydfZ"
                     /locus_tag="ACHOQV_003831"
     CDS             complement(8102..8305)
                     /gene="ydfZ"
                     /locus_tag="ACHOQV_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFP9632127.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(8482..9168)
                     /gene="rspR"
                     /locus_tag="ACHOQV_003832"
                     /gene_synonym="ydfH"
     CDS             complement(8482..9168)
                     /gene="rspR"
                     /locus_tag="ACHOQV_003832"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFP9632128.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLEIFSALQKRDGDAVERAMTHHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(9257..10003)
                     /gene="ydfG"
                     /locus_tag="ACHOQV_003833"
     CDS             complement(9257..10003)
                     /gene="ydfG"
                     /locus_tag="ACHOQV_003833"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFP9632129.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDSKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            10140..12185
                     /gene="dcp"
                     /locus_tag="ACHOQV_003834"
     CDS             10140..12185
                     /gene="dcp"
                     /locus_tag="ACHOQV_003834"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFP9632130.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQAPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDFAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELVSIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDESQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KSAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            complement(12230..>12318)
                     /locus_tag="ACHOQV_003835"
     CDS             complement(12230..>12318)
                     /locus_tag="ACHOQV_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632131.1"
                     /translation="KSNFAGDCETAFALAVKFALTQRLQLLL"
BASE COUNT         3086 a         2787 c         3159 g         3286 t
ORIGIN      
        1 ttttgggtta agaattatta atccatatca atagaagggt atgagtaata aggtgggatt
       61 atgttgtatg ttcaaatcgc cggatgtgtc gtatccggcg tgcagtcgat aatgtattac
      121 tgcggttcgg caggcgcgcc atcctgggta gactgcgcgg gagcagagac gttaccgctg
      181 gtggtgcggg tgtagagaat tttatgcgta tcattagcgc aatgcccgac gacctgggaa
      241 tcaggctgat caacctggtc attgggtaca atacttaacg tgaagctgct ttcgggtacg
      301 ccattattga taatgcgctg tgatatatcg ctctgtatgc gctcacagga tcccggcgcg
      361 gcgagtaccg cgggtgaggc gagggcgagc agaagcgcgg cacagcaggt tgagagtttc
      421 atcataagct ccttacgcga agataacttc tttaagcata gcatttaacg tgtaaagtac
      481 tgtatttgct actatgattg agaatcatct ctactctctg gtgactgttg tgaaatacaa
      541 attactgcca tgcttactcg cgatattcct cacaggatgt gaccgcacag aggtaacact
      601 ttcatttacc cctgagatgg ccagtttctc taatgaattc gattttgatc cgctgcgtgg
      661 tccggtaaaa gatttcactc agacattaat ggatgagcaa ggtgaagtga cgaaacgtgt
      721 ttcggggact ttgtcggaag aaggctgttt tgattcactc gaattactgg atctggaaaa
      781 taataccgtg gttgccctgg tactggacgc caattattac cgtgatgccc agacactgga
      841 gaagagagtt cgtttgcagg gaaaatgcca gctagcagaa ttaccttctg ccggggtgag
      901 ctgggaaacc gatgataatg gctttgtgat taaagccagc agcaaacaga tgcagatgga
      961 atatcgctat gatgatcagg ggtatccgct gggtaaaacc acgaaaagta acgacaaaac
     1021 attatctgtc agcgctacgc catcaacgga tccgatcaaa aaattagatt acacagcggt
     1081 tactttactg aataatcaaa gggttggtaa tgtaaaacag agctgtgaat atgacagtca
     1141 cgccaatccg gtggactgtc agctaatcat tgttgatgaa ggagtaaaac ccgccgtcga
     1201 acgggtttac accatcaaaa atactatcga ttattattaa tgctattgtg cggtcggctt
     1261 caggagagtc tgacccggtg ttttgtgctc tgccagatac tgatgctgga atatgcacat
     1321 gcgaatggca ttacgatatt gaccattaat aaagaactca tgcatcaatt caccttcaac
     1381 cgtaaagcca agcttgcggt aaatgtgaat cgctttttca ttctctttat cgacgatcag
     1441 atacagctta tagagattga gaacggtgaa gccatagtcc attgctaatt ttgcggcacg
     1501 cgttgccaga cctttcccct gatattccgg ggagataatt atctgaaatt ctgcgcggcg
     1561 atgaacatgg ttaatttcca ccagctccac cagaccggct ttttcgccgt cacattccac
     1621 cacaaagcgc cgttcgctct gatcgtgaat atgcttatcg tacagatcag agagttcaac
     1681 aaaggcttcg tagggttcct caaaccagta acgcatcaca ctggcgttat tgtcgagttg
     1741 atgtacatag cgtaaatctt cacgctccag cgggcgtagc ttaacactgt gggcgcttgg
     1801 cataacgtgt ccttacattc cttaaatcaa taacaggtta gggggtaata acgcggccag
     1861 ttcgacggtc caggcagcgc aaagtatttg gctcccagta ggcattgatg ttggcgcttt
     1921 gctcacattt atcgcggtta tcaaaagcgg cgtcggcttt atcccactct ttttcagtgc
     1981 gtttattcac tttctggcgc agattgcgcg tgtcattcca ttgctctttt tccatagcgg
     2041 cgtgctggcg gctttgtgca ctgtcgccag actcaatcac cagtttgtta gtttcggcat
     2101 gaacagttgt gctcaatgcc agtgcgcaag gcagcagaaa agcgagcagg ccaattcgtt
     2161 tgctgagagt aattttcata attcattccc tgtatgaatg attaaaggtg attctacacc
     2221 atccactgcg aacgcaaaac gtaccaggag gatgtttata ttgatgatat tatgtcgtcc
     2281 tataactata catgatgtca ataagtgaca aagatgatta aaacaacgtt actatttttt
     2341 gctactgcgc tgtgtgaaat tattggatgc tttctgccct ggttgtggtt aaaacgaaac
     2401 gccagtatct ggctgttgct tccggcgggg atttcactgg cgctgtttgt ctggttgtta
     2461 acgttgcatc ctgcggcgag tgggcgtgtt tacgccgctt atggtggcgt ttatgtctgc
     2521 acggcgttga tgtggctgcg cgttgtggat ggcgtgaaac tgagtcttta tgactggacg
     2581 ggggcgttga ttgcgctttg cggcatgttg atcattgttg cgggctgggg gcgcacgtag
     2641 gaacataaat cccttttatc aataagatac gaggaagtgt cagctgacaa aaggtattct
     2701 atttcatctt ttgtcaacca ttcacggcgc aaatatacgc ctttttttgt gatcactccg
     2761 gctttttttg atcttcatac ttgtatggta gtagctcagt tgcgtagatt tcatgcatca
     2821 cgacaagcga tgcaaggaat cgaacatgaa gatcgtaaag gctgaagttt ttgttacctg
     2881 tccggggcgt aatttcgtca cattaaaaat caccactgag gacggtatta cgggccttgg
     2941 ggatgccacc ctcaatggac gtgagctttc cgtggcctct tatttgcagg atcacctttg
     3001 tccgcagctt attggtcgcg atgcacaccg tatcgaagat atctggcagt ttttctataa
     3061 aggtgcttac tggcgtcgcg gtccggttac gatgtcggcc atttcagcgg ttgatatggc
     3121 gctgtgggat attaaagcca aagctgccaa catgccgctt taccagttac tcggcggcgc
     3181 gtctcgtgaa ggggtgatgg tttattgcca taccaccggt cacagtattg atgaagctct
     3241 ggatgattat gcccgtcatc aggagctggg attcaaagcc atccgcgtgc agtgcggaat
     3301 ccctggtatg aaaaccacct atggcatgtc gaaaggtaaa ggtctggctt atgaacccgc
     3361 aaccaaagga cagtggccgg aagagcagct gtggtcgacg gagaaatacc tcgatttcat
     3421 gccgaaattg tttgacgcgg tacgtaacaa gtttggtttt aatgaacatt tgctgcatga
     3481 catgcaccat cgcttaacgc ctattgaagc ggcgcgcttt ggtaaaagca ttgaagatta
     3541 tcgcatgttc tggatggaag acccgacgcc tgcggaaaac caggagtgtt tccgcctgat
     3601 tcgccaacac accgtcacgc caattgcagt gggtgaagtc ttcaacagca tctgggactg
     3661 caaacagctg attgaagaac aacttatcga ctatatccgc accacgctga ctcacgcggg
     3721 ggggattacc ggtatgcgcc ggattgccga ttttgcttcg ctgtatcagg tacgtactgg
     3781 ctcacacggt ccttccgatt tgtcgccagt ctgcatggct gcggcgctgc actttgatct
     3841 gtgggtcccc aatttcggtg tccaggagta catgggttat tccgaacaaa tgcttgaagt
     3901 cttcccgcac aactggactt tcgataacgg ctatatgcat ccgggagaca aaccgggtct
     3961 tggcatcgaa ttcgatgaaa agctggcggc gaaatatccc tatgaacctg cttatctgcc
     4021 agtcgcacgt ctggaagatg gcacgctgtg gaactggtaa ggagtaagat aatgaaaagc
     4081 attttaattg aaaaaccgaa tcaactggca attatcgaac gcgaaatacc caccccgtca
     4141 gcgggtgaag tacgagtaaa agtgaaactt gccggaattt gtggttcaga tagccatatt
     4201 tatcgtgggc ataatccttt tgcgaaatat ccgcgcgtca ttggtcatga attctttggc
     4261 gtcattgatg cagtgggtga aggcgtggaa agcgccagag tcggtgaacg cgttgctgtc
     4321 gatccggtgg tcagctgtgg gcattgctat ccgtgctcta taggtaagcc gaacgtttgt
     4381 acgacactgg ctgtattagg tgtgcacgct gacggtggtt tcagtgaata tgccgtggtt
     4441 ccggcaaaaa atgcgtggaa aattcctgaa gcagtggccg atcaatatgc ggtgatgatc
     4501 gaacctttta ccattgcggc taacgttacc ggtcatggtc aaccgactga aaatgatacc
     4561 gttctggttt acggtgccgg tccaatcggc ctgacgatcg ttcaggtatt aaaaggcgtc
     4621 tataacgtta aaaatgtgat tgttgccgat cgcattgatg aacgactgga aaaagcgaaa
     4681 gagagcgggg cagactgggc gattaataac agccagacac cgcttggcga gagtttcgct
     4741 gaaaaaggca tcaagccgac attaattatc gatgcggctt gtcatccttc tatcctgaaa
     4801 gaggctgtaa cgctggcttc tccagcggca cgtattgtat tgatgggctt ctccagtgaa
     4861 ccgtctgaag tgattcagca aggaattacc ggaaaagaac tctctatttt ctcttcacgc
     4921 ttaaatgcaa ataaatttcc ggtcgttatc gactggttaa gtaaagggtt aattaaacca
     4981 gaaaaattaa ttacccatac gtttgatttc cagcatgttg ctgatgccat tagtttattt
     5041 gaacaggatc aaaaacattg ctgcaaagtc ttacttactt tttctgaata ataccaataa
     5101 cggcgagtaa gtagtacgca tcttacctct tttttagaga taaccattat gacaatagaa
     5161 aaacacgaaa gaagcactaa ggatttggtg aaagcagcag tatcgggatg gctgggcact
     5221 gcgcttgaat ttatggattt ccagttatat tcgctcggtg cagcgttagt gtttcatgaa
     5281 atattttttc ctgaatcatc aacggcaatg gcgttaattc tggcaatggg aacctacggt
     5341 gcaggttatg tggcgcgtat tgtcggagca tttattttcg gcaaaatggg cgacagaata
     5401 gggcgtaaaa aagtactctt tattaccatc accatgatgg ggatctgtac caccttaatt
     5461 ggtgtgttac cgacctatgc acagattggt gtttttgcac ccatcttgct ggtgacgctg
     5521 cgtattattc agggattggg tgcaggtgcg gaaatttccg gtgccggtac gatgctggcg
     5581 gaatatgcgc caaaaggtaa gcgcggaatt atctcctcat ttgtggctat gggaactaac
     5641 tgcggaacct taagcgcaac ggcaatctgg gcctttatgt tcttcattct cagtaaagag
     5701 gaactgttgg cgtggggatg gcgtataccg ttcctggcga gcgttgtcgt gatggtcttt
     5761 gctatctggt tgcgtatgaa tctgaaagaa agcccggtct ttgagaaggt taacgacagc
     5821 aaccaaccga cagcaaaacc tgcacctgct ggtagcatgt tccagagcaa atccttctgg
     5881 ctggcaacag ggctgcgttt tggtcaggct ggtaactccg gtttaattca gactttcctt
     5941 gcaggctatt tagtacagac gttattgttt aacaaagcaa ttccaacaga tgcattgatg
     6001 atcagttcga ttctcggctt tatgaccatt ccgttccttg gttggttatc cgataaaatt
     6061 ggtcgccgga tcccgtatat tattatgaat acctccgcga ttgtgctggc atggccaatg
     6121 ctttctatca tcgtagataa aagctatgcc ccgagcacca ttatggtggc actgattgtg
     6181 attcataact gtgcggtgct gggattattt gctctggaaa acattaccat ggcagaaatg
     6241 ttcggctgta aaaaccgctt tacccggatg gccatttcta aagaaattgg tggtcttatc
     6301 gcttccggtt ttggtcctat cctggcgggt attttctgca ccatgacgga atcctggtat
     6361 ccgatcgcca ttatgatcat ggcatattca gtgattggtt taatctctgc gctgaaaatg
     6421 ccagaagtga aagaccgtga tttaagtgca ctggaagatg ccgcggaaga tcaaccgcgt
     6481 gttgtaacag ctgcgcaacc ttcaagaagt ctgtaaatcc tccatccctt ctcttaccgg
     6541 agaggggatt ttttattcat ataacaaaac atgcagcttg ccatatttaa atttaaggaa
     6601 ttgttatggg aaataatttg ttatcagcaa aagcgacgct ccctgtttat gatcgtaata
     6661 acctggcccc aagaattgtt catttaggct ttggtgcatt tcaccgtgcg catcagggtg
     6721 tgtatgccga tattcttgct acggaacatt tcagtgactg gggatattat gaggtcaatt
     6781 taatcggcgg cgaacagcaa attgccgatt tacaacagca agataatctt tataccgttg
     6841 cggaaatgtc ggccgatgcg tggacggctc gcgtcgttgg cgtcgttaaa aaagccttgc
     6901 acgtacagat tgatggctta gaaaccgtgt tggctgtgat gtgtgaaccg caaatcgcga
     6961 ttgtctctct gacaatcacc gaaaaagggt atttccactc tccggcgacc ggacagttaa
     7021 tgctcgatca cccgatggtc gttgccgacg tacaaaatcc ccaccagccg aaaactgcaa
     7081 caggggtgat tgtcgaggcg ctggctcgcc gtaaagcggc aggacttccc gcatttaccg
     7141 tcatgtcatg tgacaacatg ccagaaaacg gtcatgttat gcgtgacgtt gtcacttcct
     7201 acgcgcaagc tgttgatgta aaactggcac aatggatcga agaaaacgtg actttcccat
     7261 caacaatggt ggaccgtatt gtgcccgcag tgacggagga tacgctggcg aaaatcgaac
     7321 aacttaccgg tgtgcgcgat cctgcgggcg ttgcctgtga acctttccgc cagtgggtaa
     7381 tagaagataa ctttgttgcc ggacgtccgg aatgggaaaa agcgggagcc gaactggtta
     7441 gtgatgtgct gccttatgaa gagatgaagt tgcgcatgct caacggcagt cattcattcc
     7501 tggcgtatct ggggtatctt gccggatatc agcacattaa tgactgcatg gaagatgaac
     7561 attatcgtca tgcagcgtat gccttgatgt tgcaggaaca agcgccgacg ttgaaagtgc
     7621 agggcgttga tttgcaagat tacgctaacc gattaattga acgctatagc aacccggcgc
     7681 tacgtcatcg aacctggcag attgcgatgg atggtagcca gaaattgcca cagcggatgt
     7741 tggattctgt tcgctggcat ctggcgcatg acagcaagtt cgatctgctg gcgctgggcg
     7801 tcgcaggttg gatgcgttat gtcggtggtg ttgatgaaca gggaaatccg atagaaatca
     7861 gtgacccact gttacctgtt attcagaagg ctgtacaaag tagtgccgaa gggaaagcgc
     7921 gcgtccagtc attgctggcg attaaggcga tctttggtga tgatttgcca gacaatagct
     7981 tgtttactgc aaaagtgacg gaagcgtact tgtctttatt agcgcatggt gcgaaagcga
     8041 ccgtggcgaa atattccgtg aagtaaaaaa agcaatgccg ccatcggcgg cattcacacg
     8101 cttagttcat gccgagacga atcaggtcca gaggtgccag tttctcttca caaccttcaa
     8161 caactacggt ttttccgcgg cggatttgct cagcggtcag accttcagta tcaatcgaca
     8221 ggatcttgcc agtgtgaccg gtgccgctaa ccataacacg gctgccagtg gtgattgcgt
     8281 tacggttacg atcgtaggtc gtcataggtg ttttctcctt tctgatttac agtcgaagcc
     8341 cacgtaacgg gcgcactata aatactcctg tcgagtgaaa tattttttgt ttttgatcaa
     8401 gctcacatct tttttgatgc atgcatgagc catatgacgg agggaaaaac tctcaccccg
     8461 gatgggatga gaggaaatga attactcttc gctaaaccag tcgctgtttt cctgacggat
     8521 ctggcgcacg gattcgctga tttcctgcaa atggtgcgtc attgcacgtt ctaccgcatc
     8581 gccatcacgt ttttgcaggg cagagaaaat ctcaagatgc tggcgtaaca gcatttctgg
     8641 tggagaaacg tggtcgaaac tcatatagcg cacgcgatca acggtcgctt tcaggttctc
     8701 aatggtatcc cacgccagtt gacagtcggc aatctgcgtc aggagttgat ggaagttgtc
     8761 atcaagttca aaaaaatcat ccagttgctt gcgctctatg gcaatgcgtt gctggtgaag
     8821 attttgttcc agttggtagc actggctttc ggtaatcatg ctcgccgccc gacgcgccac
     8881 cgcgcactca atggcctgac ggataaaact gccgttgcgc acctgggcca tggaaatttt
     8941 gttgacgtag ctgccacgct gcggacgaat ttgaatcagg ccgttttccg ccagtttaat
     9001 aaaggcttca cgaaccggct ggcgtgacac attgaaacga acagaaactt ctttttccga
     9061 caacggtgtg cctggtgcaa tcaggcaatg gacaatgtcg cgacgaagaa tacgataaat
     9121 ctgctgattg acaggctgtg tgggattaag ttgcgtttcg acggtcattc gttcttgctt
     9181 gtgagtgagt taactgcatg agtctaccac ttttttgtga caagcaataa cccggcagtt
     9241 acgccgggta taaaaattac tgacggtgga cattcagtcc ggcatagctt tgggtaaccg
     9301 gcatcatttc cagggtattg atattgacgt gagcaggcag cgttgacacc caccagacgg
     9361 cttcgctgac atcttccggc gtcaatgcaa cggtattttg ataggttttt tccgctttac
     9421 tgtcatcgcc tttaaagcgg acattggaaa actcggtgcc acccaccaga cccggttcga
     9481 tgtcggtgac gcgcaccgcc gtaccatgca ggtccgtacg cagattcagg ctaaactgac
     9541 gaacaaacgc tttcgtcgca ccgtaaacgt taccaccggc atacggccag ctgcctgccg
     9601 ttgagccaat gttaataata tgaccatgat tacgttcaac catacccggt aagacggcgc
     9661 gcgtcatata taccaggcct ttgttgttgg tatcaatcat cgtttcccag tcttcaacgc
     9721 tggctttatg cgcaggctcc atgcccaacg ccaggccggc attatttacc aggatatcaa
     9781 tattgcacca ctcggcagga agcgatgcca gcatctcttc aatagcggcg cggttgcgaa
     9841 cgtccagttg ggcgatatac agattatctc ccagttcgtc ttttaactcc tgcaaccgct
     9901 cctggcgacg gccagtggcg ataactttat gcccttgttg aataaaacga cgagtaatgc
     9961 attcaccaaa acctgccgtt gctccagtta ctaaaacgat catctcactg ttcctcaacg
    10021 cttttgtgtg tcaataacat agcacgtgat gcttaagagc gggtaatact ggtttgcaag
    10081 attgcactcc gttatcagta agcctacgct atggaacacc tcgcgttcag gagaagaaaa
    10141 tgacaacaat gaatcctttc cttgtgcaaa gcacactgcc gtatctggct ccccattttg
    10201 atcaaattgc caatcatcac tatcgcccgg cattcgatga gggaatgcag caaaagcggg
    10261 cagaaattgc tgccatcgcg cttaacccgc aagcgcctga tttcaacaat actattctgg
    10321 cactggaaca aagcggagaa ttacttaccc gcgttaccag cgtctttttt gcgatgactg
    10381 cggcgcatac taatgatgaa ttacagcgtc ttgatgaaca gttttccgct gaactggcgg
    10441 aactggctaa tgatatctat ctgaacggtg aattattcgc gcgggtagat gctgtctggc
    10501 aacgccgtga atccctggga cttgatagtg aatccatccg cctggtggag gtcattcatc
    10561 aacgttttgt ccttgccgga gccaaacttg cacaagctga taaagcaaaa ttaaaagtac
    10621 tgaatacaga agctgcgacc ctgaccagcc agtttaacca gcggttactg gcagcaaata
    10681 aatccggcgg tctggttgtg aacgatttcg cgcagctggc aggaatgagt gagcaagaga
    10741 ttgcgctggc ggcagaggcg gctcgcgaga aaggtctgga taacaaatgg ctgattccgt
    10801 tgctgaatac cacccagcaa ccagcgcttg ctgaactgcg cgatcgcgcg acgcgtgaaa
    10861 aactgttcac tgcgggctgg acgcgagcgg aaaaaaatga tgccaatgat acccgcgcta
    10921 tcattcaacg tctggtggag atccgcgcgc agcaggcgaa actgcttggt ttccctcatt
    10981 atgccgcatg gaaaatcgcc gatcagatgg caaaaacgcc agaagcagca ctcaacttta
    11041 tgcgggaaat tgttccagcg gcgcgtcaac gtgcgagcga tgaattagtc tccatacagg
    11101 cggttattga taagcagcag ggcgggttta gcgcgcagcc gtgggactgg gcattttatg
    11161 ccgagcaggt aagacgtgag aaattcgatc tcgatgaatc gcagctcaag ccatattttg
    11221 aattaaacac ggtgttgaat gaaggtgtat tctggaccgc gaatcagctc ttcggtatta
    11281 agtttgtcga acgttttgat attcctgtct accatcctga cgttcgtgtg tgggaaattt
    11341 ttgatcataa tggcgtgggg ctggcgttat tttacggtga tttcttcgcc cgtgattcaa
    11401 aaagcggcgg tgcatggatg ggcaattttg ttgagcaatc aacgctgaat gaaacgcatc
    11461 cggtaattta taacgtctgc aattatcaga aatccgccgc tggggagcct gcgttgttac
    11521 tctgggatga tgtcataacc ttattccatg aatttggtca tacgctgcac ggcctttttg
    11581 cccgccagcg ttatgccacg ctttccggca ccaacacgcc gcgtgatttt gtcgaatttc
    11641 cgtcgcaaat caacgaacac tgggcaacgc atccgcaggt attcgctcgc tacgcccggc
    11701 attatcagag cggggcagca atgcctgatg aactgcaaca gaaaatgcgt aatgccagcc
    11761 tgttcaataa agggtatgag atgagcgaac tgcttagcgc cgcacttctc gatatgcgct
    11821 ggcattgcct ggaagaaaac gaagcaatgc aggatgtcga tgattttgaa ttgcgggcgc
    11881 tggtggcgga aaatatggat cttcctgcta taccgccacg ctatcgcagc agttatttcg
    11941 cccatatttt tggtggcgga tatgccgcag gttattacgc ttatctgtgg acgcaaatgt
    12001 tggccgatga tggttaccag tggtttgttg agcagggcgg attaacgcgt gaaaatgggc
    12061 ggcgttttcg cgaggcgatt ctttccagag gtaacagcga agatctggaa cgcctgtatc
    12121 gacaatggcg cggtaaggca cctcagatta tgccgatgct gcaacatcgt ggcttgaacg
    12181 tataaacacc accaatggct ggcctggatt tatcgccaga ccagcctcgt tatagcagta
    12241 attgcagccg ctgggtgagg gcaaacttca ccgctaatgc gaatgccgtt tcgcagtcgc
    12301 cagcaaaatt actttttt
//
LOCUS       DAZKGF010000143        12305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZKGF010000143 DAZKGF010000000
VERSION     DAZKGF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.143"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(78..773)
                     /gene="aqpZ"
                     /locus_tag="ACHOQV_003836"
     CDS             complement(78..773)
                     /gene="aqpZ"
                     /locus_tag="ACHOQV_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFP9632132.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAAAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1199..2857
                     /gene="ybjD"
                     /locus_tag="ACHOQV_003837"
     CDS             1199..2857
                     /gene="ybjD"
                     /locus_tag="ACHOQV_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFP9632133.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2854..3810)
                     /gene="ybjX"
                     /locus_tag="ACHOQV_003838"
     CDS             complement(2854..3810)
                     /gene="ybjX"
                     /locus_tag="ACHOQV_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFP9632134.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYKGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3961..5076
                     /gene="macA"
                     /locus_tag="ACHOQV_003839"
     CDS             3961..5076
                     /gene="macA"
                     /locus_tag="ACHOQV_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFP9632135.1"
                     /translation="MKKRKTVKKRYVIALVIIIVGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5073..7019
                     /gene="macB"
                     /locus_tag="ACHOQV_003840"
     CDS             5073..7019
                     /gene="macB"
                     /locus_tag="ACHOQV_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFP9632136.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7092..7316)
                     /gene="cspD"
                     /locus_tag="ACHOQV_003841"
     CDS             complement(7092..7316)
                     /gene="cspD"
                     /locus_tag="ACHOQV_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFP9632137.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7466..7540
                     /gene="yljB"
                     /locus_tag="ACHOQV_003842"
     CDS             7466..7540
                     /gene="yljB"
                     /locus_tag="ACHOQV_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFP9632138.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7639..7959
                     /gene="clpS"
                     /locus_tag="ACHOQV_003843"
     CDS             7639..7959
                     /gene="clpS"
                     /locus_tag="ACHOQV_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFP9632139.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7990..10266
                     /gene="clpA"
                     /locus_tag="ACHOQV_003844"
     CDS             7990..10266
                     /gene="clpA"
                     /locus_tag="ACHOQV_003844"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFP9632140.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            10963..12171
                     /locus_tag="ACHOQV_003845"
     CDS             10963..12171
                     /locus_tag="ACHOQV_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9632141.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAEAEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYVFPQLGAKTLDTITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQSRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRIWTIPAERMKARI
                     QHRVPLSRQAIHVLENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAISDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLDQRIPMMQAWADY
                     VMSQIMENQR"
BASE COUNT         3122 a         2850 c         3356 g         2977 t
ORIGIN      
        1 ttttgacgcc accaacaccc gtgcggtttg taacgtcgaa gataaaacaa ccagggcaga
       61 taaaatgcca atgctaatta attacgcttt tccagcaggg tccggtaaat cagaccaccg
      121 ataataccgc cgacaattgg caccacccag aagaaccaca gttgttctaa cgcccagccg
      181 ccctggaaga tagcagccgc ggtgctgcgc gccgggttaa cagaagtgtt agtcaccgga
      241 atactgatta agtgaatcag ggttaaggcc agaccaatag cgatcggcgc aaaaccagca
      301 ggagcgaatt tgtcggttgc gccgtggatc accagcagga aacctgcact caataccagt
      361 tcaactacca gcgcggaaag catggaataa ccgcctggcg aatgctcgcc ataaccgtta
      421 gaagcaaaac cgctggctgc cgcgtcaaaa cctgttttac cactggcaat taaatacagc
      481 agcgccgccg caacaatacc gccgacaacc tgggcaatta cgtagccaac gacttctttt
      541 gccgggaaac gtccgccagc ccataaacca atagtgaccg ccgggttaaa atgaccacca
      601 gaaatatgac caacagcaaa ggccatcgtc agaacggtca gaccgaatgc cagcgccacg
      661 ccggcaaaac caatgcctaa ttccgggaag cctgcggcca gtacggcact accacagcca
      721 ccaaaaacaa gccagaaagt accaaaacat tcagctgcta attttctgaa catatccacc
      781 tcaattaaaa attgaccctg tgaaaaatat ggtcgtttta tagggtcgtc gtaaaaaagt
      841 gacgacggaa ataatgcgcg gctattttaa aaacgaaggc gagtcattca ccagataaat
      901 aaatccagta aatttgattt aaggcaacag cgggttgccc catatagtca tttgtctgat
      961 tgacagtgta gtgcaagcga aagatttaat cctttaggcg taataaaaaa taatttatca
     1021 tgctaattat ttgattttgt tgtttttgca gacttatcag caagagggag tataacgcga
     1081 ttattcgctc atttttcaga catttgccat gcttaaatgt gatgtcatca cgtattagca
     1141 tggcctttcc cgttatactg ccagcgtaaa ggataagtca catatttctg gaggggatat
     1201 gattcttgag cgcgttgaaa ttgtgggttt tcgcggtatc aaccgtttgt cgttgatgct
     1261 ggaacaaaac aacgtcctga ttggggagaa cgcgtggggt aaatccagct tgctggacgc
     1321 cttaaccctg ctgctatcgc cagaatcaga tctctaccat tttgagcgcg acgatttctg
     1381 gttcccgccg ggagatatca acgggcgaga acatcatctg catattattt tgaccttccg
     1441 cgaatcgctg ccaggccgac atcgggttcg ccgctatcgg ccgctggaag cgtgctggac
     1501 gccatgcacc gatggttatc accgtatttt ttatcgtctg gaaggggaga gtgcggaaga
     1561 cggcagcgtg atgacactgc gcagttttct cgataaagac ggacatccga ttgatgttga
     1621 ggatattaac gatcaggctc gtcatctggt gcgtttaatg ccggtgctgc gcttgcgtga
     1681 tgcccgtttt atgcgccgta ttcgtaacgg cacggtgcca aatgtcccca atgtggaagt
     1741 caccgcgcgc cagctcgatt tcctcgcccg tgaattatcc tcacatccgc aaaatctctc
     1801 tgatgggcag attcgtcagg gactttccgc aatggtgcaa cttcttgagc attatttttc
     1861 tgagcaggga gctggacagg cgcgatatcg tttaatgcgg cggcgagcca gcaatgagca
     1921 acgaagctgg cgctatctgg atatcatcaa ccggatgatt gaccgacctg gagggcgctc
     1981 gtatcgggtt attttgctcg gcctgtttgc tactttattg caggcaaaag gcacattgcg
     2041 actggataaa gacgcccgtc cattgttgct gatcgaagat ccagaaaccc gtttacaccc
     2101 cattatgctt tcagttgcct ggcatctgtt gaatcttctg cccttgcagc gcattgccac
     2161 caccaactcg ggtgagttac tttcgttaac gccggtagag catgtttgcc gactggtacg
     2221 tgagtcctcg cgcgttgccg cctggcgtct ggggccgagt ggcttgagta ccgaagatag
     2281 ccgacgcata tcctttcaca ttcgttttaa ccgtccgtca tcgctgtttg ctcgctgctg
     2341 gctgctggtg gaaggggaaa cggaaacctg ggttatcaat gaactggcgc gtcagtgcgg
     2401 acatcatttt gatgccgaag gaatcaaggt cattgagttt gcccagtccg ggctaaagcc
     2461 actggttaaa tttgcccgcc gaatggggat tgaatggcat gtactggtcg atggcgatga
     2521 agcagggaag aaatatgccg ctacggtacg cagcctgttg aataacgatc gggaagccga
     2581 acgagaacat ttaacggcgt taccggcgct ggatatggaa cattttatgt atcgccaggg
     2641 attttccgat gtgttccacc gcgtggcgca aatcccggaa aatgtaccga tgaatctgcg
     2701 caaaattatc tcgaaagcga tccatcgctc ttccaaaccc gatcttgcca ttgaagtggc
     2761 aatggaggcc ggacgtcgtg gtgtggactc cgtaccgacg ctgctgaaaa aaatgttctc
     2821 acgcgtgctg tggctggcgc gcggtcgcgc ggattaaccg cgaaacatcg tggccatttg
     2881 tggctgaata gcgtcgagca tctcatagcg ccgacggtat tcagcccgtt ttttactggc
     2941 gatttcggca atctcttttc gtgctatctg tgccggaagg cggtaatggc gttcagcatc
     3001 acatacgccg ccaaccgact cccagaacgc gttgtaatcg gcatggatct tgccttcttt
     3061 atcacgataa cgcaggctgc ggtaaatatg cgtttcattg ctgacggcaa taatctgctc
     3121 tacctgcaaa cgttgggcaa acagacaggc cgcttccatc acaaggcgtt tgggaaatag
     3181 cccgtggcag gctttcgtcg cattctggat ttcctgatgt ggaatttccc attttgcgcc
     3241 ttgcagtccg ccaataaaca ttgttctttt ccccttatat tcacacaggg taaacgtgat
     3301 ctctgccaga ggaatacctt cgctgttgcg gaacaggatt gtgctgtcac cttctttatc
     3361 cattgagatc atcatggtca gctcaagcgt gaactgctcg ccgtttttgc cttccagttt
     3421 agccagttgc agcccggggg tattcaaata taagctgaat tcttccgccg acatacaccc
     3481 gcggagtaac gcataatggt aacgtaacgc ctccagcaat tgcttacggc taagattcgc
     3541 cgcaaggtaa gggcgatgca gacgcacagg cagtcgcggc tggcgcgtta acaacacatt
     3601 gagattgggc cagtgggaaa gttcgttcat ccactcaacg cttaaacgcg gcataatcaa
     3661 cgagcgcagc aaaaatttct ggcgaaaact acggcgatgc cagaatttac ccggccgaca
     3721 ctgtccacgt gccagactaa gaaaaagtga caggctgctg agagattcag atggcgtaaa
     3781 ggtccgttca gttagctgcg acatattcat gaaatcaatg gttatacatg acgtcgattt
     3841 caccattgcg tatcttaacc aaacatcaat agtgtgatta ctaatgtaaa ttttagggtt
     3901 ttgttgatat ttcgttgaag ttaatgaccc ggattggcat atggagtatt cagaatattt
     3961 atgaaaaagc ggaaaaccgt gaagaagcgt tacgttattg cgctggtgat aatcatcgtc
     4021 gggctgatta cgttatggag aattcttaac gcacccgtgc cgacttatca gacgctgatt
     4081 gtgcgccccg gtgatttaca gcaaagcgtg ctggcgaccg gaaagctgga cgcgctgcgt
     4141 aaggttgatg tgggcgcgca ggtcagcggt cagttgaaaa cgctgtcggt ggcgattggc
     4201 gataaagtaa aaaaagacca gcttttaggg gttattgatc ctgaacaggc tgaaaaccag
     4261 attaaggagg tggaagcaac gctgatggag ctacgcgcgc agcggcagca ggcggaagcg
     4321 gagctgaaac tggcgcgggt gacgtattcc cgtcagcaac gtcttgcaca aacgcaagcg
     4381 gtttcacagc aggatctcga taacgccgcg acggagatgg ctgtgaaaca ggcgcaaatt
     4441 ggcaccattg acgcgcaaat caagcgcaat caggcctctc tcgatacggc taaaaccaat
     4501 ctcgattaca cccgcatcgt tgccccgatg gccggggaag tcacgcaaat caccactctg
     4561 caaggccaga cggtgattgc cgcacaacaa gcaccgaaca ttctgacgct ggcagatatg
     4621 agcaccatgc tggtaaaagc gcaggtttct gaagcggatg taatccacct gaagccgggg
     4681 caaaaagcct ggtttacggt gcttggcgat ccactgacgc gctacgaggg gcaaatcaag
     4741 gatgtgctac cgacgccgga aaagattaac gacgctattt tctattacgc ccgttttgaa
     4801 gtacccaacc ccaatggttt actgcggctg gatatgaccg cgcaagtgca tattcagctc
     4861 accgatgtga aaaatgtgct gacgatccct ctgtcggcgt taggcgatcc ggttggcgat
     4921 aatcgttata aagtcaaatt gttgcgtaat ggtgaaacac gcgagcgtga agtgacgatt
     4981 ggcgcacgta acgataccga tgttgagatt gtcaaagggc tggaagcggg cgatgaagtg
     5041 gtgattggtg aggccaaacc aggagctgcg caatgacacc cttgctcgaa ttaaaggata
     5101 ttcgtcgcag ctatcctgcc ggtgatgagc aggttgaggt gctgaagggc atcagcctcg
     5161 atatttatgc tggtgagatg gtggcgattg tcggcgcttc gggttccggt aaatcgaccc
     5221 tgatgaatat tctcggctgc ctggataagg ccaccagcgg cacctatcgc gtagccggtc
     5281 aggatgttgc cacgctggac gccgatgcgc tggcgcaact acgccgcgag catttcggct
     5341 ttattttcca gcgttaccat ttgctttcgc atttaaccgc cgagcagaac gttgaagtac
     5401 ccgccgtcta tgccggtctt gagcggaaac agcggctgct tcgcgcccag gagttgctgc
     5461 aacgactggg gctggaagac cgtacagagt attacccggc acagctttcg ggtggtcagc
     5521 aacagcgcgt cagcatagcg cgggcattga tgaacggcgg tcaggtaatt cttgccgatg
     5581 aaccgaccgg cgcactggac agccattctg gcgaagaggt gatggcgatc ctgcatcagc
     5641 tgcgcgatcg cgggcatacg gtgattatcg ttacccacga tccgcaggtc gctgctcagg
     5701 ccgagcgggt gatcgaaatt cgcgacggtg aaattgtgcg caatcctccc gccattgaaa
     5761 aagtgaatgt tgctggcggg acggaacctg ttgtcaacac ggcgtctggc tggcggcagt
     5821 ttgtcagcgg ttttaacgag gcgctgacga tggcatggcg ggcgctggca gcgaataaaa
     5881 tgcgtacttt actgaccatg ctggggatta ttatcggtat tgcgtcggtg gtttccattg
     5941 tcgtggtggg tgacgccgcc aaacaaatgg tgctggcgga tattcgttct attggtacga
     6001 atactattga tgtctatccc gggaaagatt ttggcgatga cgatccgcaa tatcagcagg
     6061 cgctgaagta cgacgactta atcgccatcc aaaaacaacc gtgggtcgcc tcagccacac
     6121 ctgccgtctc gcaaaacctg cgcctgcgtt ataacaatgt tgatgtggct gccagtgcca
     6181 atggcgtgag cggcgattat tttaatgtct atggcatgac cttcagtgaa gggaacacct
     6241 ttaatcagga gcagctgaac ggtcgtgcgc aggtggtggt gctcgacagt aatactcgcc
     6301 gccagctttt cccccataaa gcagatgtgg ttggcgaggt gattctggtc ggcaatatgc
     6361 ccgccagagt cattggtgtg gcggaagaaa aacagtcgat gtttggtagc agtaaagtgc
     6421 tgcgtgtctg gctaccttac agcacgatgt ccgggcgagt tatgggccag tcgtggctga
     6481 actccattac tgtcagggtg aaagaaggct ttgacagcgc tgaggcggaa cagcaactca
     6541 cgcgtttact ttcactgcgc cacggtaaga aggatttctt tacctggaac atggacggcg
     6601 tcttgaaaac cgttgaaaag accacacgta ctttacaact gtttctgacg ctggtggcgg
     6661 tgatttcgct ggtggtgggc ggtattggtg taatgaatat tatgctggtg tcagtgaccg
     6721 agcggacgcg ggaaattggc attcgcatgg ctgtaggtgc gcgagcaagc gatgttttgc
     6781 aacagttcct gattgaagcc gtactggttt gcctggtcgg tggcgcgttg ggaataacac
     6841 tgtcactgtt aattgctttc accttacagc ttttcttacc cggctgggag attggttttt
     6901 caccgttggc gctgctgctg gcgtttctct gctcgacggt caccgggatt ttatttggct
     6961 ggttacccgc acgaaatgcg gcacgactgg atccagtaga tgctctggca cgagagtaat
     7021 ttttgagata aaaatgccag ccgatcgggc tggcattttg cctttaggat gtacacaatg
     7081 agacagaaga gctatgcgac tgccgcttct acttcgacgg gcacaataac actggcgtga
     7141 ttgccttttg gcccctggtg gacatcaaac tgaacggatt gtccagcttt tagcgttctg
     7201 taaccatcca tctgaatggt ggaataatga gcgaaaatat cttcgccgcc gccttcaggg
     7261 cagatgaaac caaacccttt ggcattgttg aaccacttaa cagtaccctt ttccatgctt
     7321 cgacatcctt cgcaaatctt atacaagtaa gatggaataa accggggtca gagagggggc
     7381 tgttcaaaac ctcgccaact ctagaaatac aatttagaga attagggcga gccgtcaagc
     7441 atttgacagg ggccaggggg caggtatgaa tcaaaaattt gaagcagtta acgctattga
     7501 caggaatgtg acagatgtcg ctgatgccaa cgatagatga tagttatcta tcatgtggag
     7561 tagattggtc aggcaaataa gctcttgtca gcggcagggc gttctgccga taaccgtaac
     7621 cgaagatgat aactgacaat gggtaaaacg aacgactggc tggactttga tcaactggcg
     7681 gaagaaaaag ttcgcgacgc gctaaaaccg ccatctatgt ataaagtgat attagtcaat
     7741 gatgattata ctccgatgga gtttgttatt gacgtgttac aaaaattctt ttcttatgat
     7801 gtagaacgtg caacgcaatt gatgctcgct gttcactacc aggggaaggc catttgtgga
     7861 gtctttaccg ctgaggttgc agaaaccaaa gtggcgatgg tgaacaagta cgcgagggag
     7921 aatgagcatc cattgctgtg tacgctagaa aaagcctgaa tgcaggcata aaaattgggg
     7981 gaggtgccta tgctcaatca agaactggaa ctcagtttaa atatggcttt cgccagagcg
     8041 cgcgagcacc gtcatgagtt tatgaccgtc gagcacttgt tactggcgct gctcagtaac
     8101 ccatctgccc gggaggcgct ggaagcgtgc tctgtggatt tggttgcgct ccgtcaggaa
     8161 ctggaagcct ttattgaaca aaccacaccc gttctgcctg ccagtgaaga ggagcgcgac
     8221 acacagccga cgctgagttt tcagcgtgta ctgcaacgtg cggtcttcca tgtccagtcc
     8281 tccggtcgca atgaggttac cggtgcaaac gttctggtcg ctatctttag cgaacaggag
     8341 tcgcaggcgg catatctgtt gcgcaaacat gaagtcagcc gtctcgatgt ggtgaacttt
     8401 atctctcatg gcacgcgtaa agacgagccg acacagtctt ctgatcctgg cagccagcca
     8461 aacagcgaag aacaagctgg tggggaggaa cgtatggaga atttcacgac gaacctgaat
     8521 cagcttgcgc gagtgggcgg aatcgaccca ctgattggtc gtgagaagga gctggagcgt
     8581 gctattcagg ttctctgccg tcgccgtaaa aacaacccgc tgctggtggg ggaatctggt
     8641 gtcggtaaaa ccgcgattgc ggaggggctt gcatggcgaa ttgttcaggg cgatgtgccg
     8701 gaagtgatgg ctgactgtac gatttactct ctcgatatcg gttctctgtt agcgggcaca
     8761 aaatatcgcg gcgactttga aaaacgtttt aaagcgttgc tcaagcagct ggagcaagac
     8821 actaacagca tcctgtttat tgatgagatc cacaccatta tcggtgcggg tgcagcgtct
     8881 ggtggccagg tcgatgcagc taacctgatc aaaccgttgc tctccagcgg taaaattcgc
     8941 gtaattggtt cgacaaccta tcaggagttc agcaacattt tcgagaaaga ccgtgctctg
     9001 gcgcgtcgct tccagaaaat tgatattact gaaccgtcaa tcgaagaaac tgttcaaatc
     9061 atcaatggcc tgaaaccgaa gtatgaagcg caccacgacg tgcgttatac cgcaaaagcg
     9121 gtgcgtgcag cggtagagct ggcggtgaaa tacattaacg atcgtcatct gccggataaa
     9181 gccattgacg ttatcgacga agcgggcgct cgcgcacgcc tgatgccggt aagcaaacgc
     9241 aagaaaaccg ttaatgtggc ggatattgag tccgtggtgg cccgtattgc gcgcattcca
     9301 gagaagagtg tttcgcagag tgaccgcgat accctgaaaa acctcggcga tcgcctgaaa
     9361 atgctggtct tcggtcagga taaagccatt gaggcgctga ctgaagccat taagatggcg
     9421 cgtgcaggtt taggtcacga acataaaccg gtcggttcgt tcctgtttgc cggtcctacc
     9481 ggggtcggga aaacagaggt gacggtacag ctttcgaaag cgttgggcat tgagcttctg
     9541 cgctttgata tgtccgagta tatggaacgc cataccgtca gccgtctgat tggtgcgcct
     9601 ccgggatacg ttggttttga tcagggcggt ttgctgactg atgcggtcat caagcatcca
     9661 catgcggtgc tgctgctgga cgaaatcgag aaagcgcacc cggacgtgtt caatattctg
     9721 ttgcaggtga tggacaacgg tacgctgacc gataacaacg gacgcaaagc ggacttccgt
     9781 aacgtggtat tggtgatgac caccaacgct ggggtacgag aaactgagcg taaatcgatt
     9841 ggtcttatcc accaggacaa cagtaccgat gcgatggaag agatcaagaa gatctttacg
     9901 ccggagtttc gtaaccgtct cgacaacatt atctggttcg atcatctctc caccgacgtg
     9961 atccatcagg tagtggataa attcatcgtc gagttgcagg ttcagctgga tcagaaaggt
    10021 gtttctctgg aagtgagcca ggaagcgcgt aactggctgg ccgagaaagg ttacgaccgg
    10081 gcaatgggcg cacgtccgat ggcgcgtgtc atccaggaca acctgaaaaa accgctcgcc
    10141 aacgaactgt tgtttggttc gctggtggac ggcggtcagg tgacggttgc gctggataaa
    10201 gagaaaaatg agctgactta tggattccag agtgcacaaa agcacaaggc ggaagcagcg
    10261 cattaatcta attgttaggt aggttggtga agtccgtaat ctcgaaagag gttacggact
    10321 ttttgtttat ggggtggagg aggttctgac cctttttttg atgatgatgg taatttgttg
    10381 ataattagtg ctgcgggaag gtaaggataa aaaagggtgg tagcaggaaa atgagatggt
    10441 tttgctttat taacaacggg ctagacgtgt agtatttgag ttcactgccg tacaggcagc
    10501 ttagaaagcc aaccgaaaga aaaactcgat ttttataccg ttcactgccg tacagacaga
    10561 taaaatgcga aaaaaaaaac gtactttcgt acgagctctt ctttgaatat ggcggtgagg
    10621 gggggattga ctcgcttcgc tcgccctgcg ggcagcccac tcactgtgtt cgtggtctgt
    10681 ccaactggct gcgccagttg tcgaaccccg gtcggggctt ctcatccccc ctggagtgca
    10741 atatgcgaaa aaaaagctcg cactttcgta cgagctcttc tttgaatatg gcggtgaggg
    10801 ggaccgcctg aaataaatct gacttaatcc gggagtgtta tataacttca ttataaacaa
    10861 tgcattacaa tttttgctgt ccgatgttat ctaatgtatt cttagtgaag tgagagacta
    10921 atggcatact ggatggcata ccagagtaac aggaattagc gtatggcagt attgacggat
    10981 actaaagcaa ggcatatcaa acctgatgac aaaccattgc cccatggggg aattacagga
    11041 ctgactcttc atccttcttc agtaaagggg cgggggaaat gggtttttcg ttatgtaagt
    11101 ccggtgacac aaaaaaggcg taatgctgga ttgggtactt accctgaggt cagtattgct
    11161 gaagctgcac gtactgcccg gataatgcga gagcaacttg ctgcaggtga tgatcctctg
    11221 gagattaaaa aggctgaagc tgaaaaagtt gttatcccaa catttgccga tgcagcccgg
    11281 cgtgtacatg cagaattgtc accgggatgg gaaaacccaa agcatgtcag gcagtggttg
    11341 tcgacgcttg aaaattacgt gtttcctcaa ctgggagcaa aaacgctgga tacgattacg
    11401 gctgcggacg tggcagagac actgcgtcca gtctggttga ccttatcaga aacggcaagc
    11461 cgggttaaac agcgcattca tgttgttatg cagtggggct gggcgcatgg tttttgtgtg
    11521 gcgaatcctg ttgatgtggt tgatcatttg cttccacagc aatcaagagg acgtgatgaa
    11581 caccagccgg caatgccctg gaggcagtta ccgctttttg tggcgaccag tgtgtataca
    11641 gatgaacctt ataatgttac ccgggcactg ttattaatgg tgatactgac agcaacccgc
    11701 tcgggcgaag caaggggaat gcgctgggct gaaattgatt ttcataagcg gatatggacg
    11761 atacccgcag aaagaatgaa agccaggata cagcatcgtg ttcctttatc ccgacaggcc
    11821 attcacgttc tggaaaatat acgtggtctg catgacgaac tggtgtttcc ttctcccaga
    11881 aagcagcaga tcctttcaga tatggtgttg acgagttttc tacgtaaaaa gaaggccatc
    11941 agtgatatac ccggacgagt ggctacagca catggttttc gttcaacatt cagggactgg
    12001 tgtagcgaac agggatattc gcgggatttg gcggaaaggg cgcttgccca cacgctgaaa
    12061 aataaggttg aggcggcata tcaccggact gatctgctgg atcagcgtat accgatgatg
    12121 caggcatggg cggattatgt gatgtctcag attatggaaa accagcgatg aaatcacatc
    12181 tcacagattt gcagatgtcg tctgtaatca cgaaaaatat acttattgag ttataatgct
    12241 tgtttgtctg atatttgcaa tgacataaat gtaaaaataa tacgtaatat tatagtttat
    12301 aaatt
//
LOCUS       DAZKGF010000144        12043 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZKGF010000144 DAZKGF010000000
VERSION     DAZKGF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.144"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(48..956)
                     /gene="galU"
                     /locus_tag="ACHOQV_003846"
     CDS             complement(48..956)
                     /gene="galU"
                     /locus_tag="ACHOQV_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9632142.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1158..2171)
                     /gene="rssB"
                     /locus_tag="ACHOQV_003847"
     CDS             complement(1158..2171)
                     /gene="rssB"
                     /locus_tag="ACHOQV_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFP9632143.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2263..3168)
                     /gene="rssA"
                     /locus_tag="ACHOQV_003848"
     CDS             complement(2263..3168)
                     /gene="rssA"
                     /locus_tag="ACHOQV_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFP9632144.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            3281..3739
                     /locus_tag="ACHOQV_003849"
     CDS             3281..3739
                     /locus_tag="ACHOQV_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFP9632145.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMK
                     DADYLIKTWHPSCGAATLRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARFTE
                     GSKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3789..4631
                     /gene="purU"
                     /locus_tag="ACHOQV_003850"
     CDS             3789..4631
                     /gene="purU"
                     /locus_tag="ACHOQV_003850"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFP9632146.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4791..4875
                     /locus_tag="ACHOQV_003851"
     tRNA            4791..4875
                     /locus_tag="ACHOQV_003851"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4825..4827,aa:Tyr,seq:gta)
     gene            4922..5053
                     /locus_tag="ACHOQV_003852"
     ncRNA           4922..5053
                     /ncRNA_class="other"
                     /locus_tag="ACHOQV_003852"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5238..5915)
                     /gene="narI"
                     /locus_tag="ACHOQV_003853"
     CDS             complement(5238..5915)
                     /gene="narI"
                     /locus_tag="ACHOQV_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP9632147.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVQVEYLTRKY
                     QLVRARH"
     gene            complement(5915..6625)
                     /gene="narJ"
                     /locus_tag="ACHOQV_003854"
     CDS             complement(5915..6625)
                     /gene="narJ"
                     /locus_tag="ACHOQV_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP9632148.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFAEKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(6622..8160)
                     /gene="narH"
                     /locus_tag="ACHOQV_003855"
     CDS             complement(6622..8160)
                     /gene="narH"
                     /locus_tag="ACHOQV_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP9632149.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(8157..11900)
                     /locus_tag="ACHOQV_003856"
     CDS             complement(8157..11900)
                     /locus_tag="ACHOQV_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFP9632150.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLLGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLMGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQALDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITSQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         2858 a         3286 c         2965 g         2934 t
ORIGIN      
        1 ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat acggatgtta cttcttaatg
       61 cccatctctt cttcaagcca ggctttaaat tccgtaccaa gggtgttatg acgaataccg
      121 tattcaacga aggcctgcat gtaacctaat ttattaccac agtcatggct cttccctttc
      181 atatgatagg cttccaccgt ttctttttcg atcagcatat caattgcgtc ggtgagctga
      241 atttcatcac cagctcccgg aggggttttt gccagcaacg gccaaatatc cgcgctaagt
      301 acgtaacgac ccacaatagc gagattagac ggcgcaacat ccgcttttgg tttttcaacc
      361 acaccaacca tcggtacgct ttcacccggc gctaattcaa cgcctttgca atccacaacg
      421 ccatatgcgg taacatcagc aaccggttca accatgatct ggctatgacc cgtttcatca
      481 aagcggcgga tcatctctgc caggttatcc tgtgacaaat cggattcata ttcatccaga
      541 ataacgtcag gcaaaataac agccacaggt tcatcaccca ctaccgggtg agcacacaat
      601 accgcatgtc ccaggccttt cgccagcccc tgacgaactt gcataatagt cacgtgcggc
      661 ggacaaatag actgcacttc atcaagcagt tgacgtttta cacgtttttc cagcattgct
      721 tccagttcaa aactggtatc aaagtggttt tcaatagagt ttttagatga gtgagtaacc
      781 agcacaattt cagtaatgcc agccgcaata cattcattca cgacgtattg aattaatggc
      841 ttatcgacaa gtggcagcat ctctttcggg atggctttcg tcgccggcaa catcctggtt
      901 cctaatcccg caacggggat aacggctttt ttgactttcg tattaatggc agccatttaa
      961 attctcctgg actgttcgtg ttttgaacgt gttcatattt ctgtatcgca tcccagtata
     1021 tcagcacccc gaaggggcta agttctgaaa aggaggcgag ttagcataat taagacaatt
     1081 acttatacga atggcgacga tagtaccacc agccgacatt agcaggtaat gcaaatttag
     1141 cccgcgttat cgtttgctca ttctgcagac aacatcaagc gaagccgacc accggttccc
     1201 catatttggc attgccaggc atcgcatcgc tggctcaatt gattcaaata agcgttacct
     1261 aaagtgccta acggaacacc attactgatt tgcacctggt gttcgccggt atttaacgtc
     1321 gcattcagac ccgcagaaac cagaatgaga tttttcagtt cgcgatgata atagccaact
     1381 aatagcggaa actgccccgg cagattggcc tgacggagta aatggtttac ctgcttcaat
     1441 aacgcgccca actctggcaa acgttgattt tggtgtgcaa gctgttcctg taataatccg
     1501 ttaaacaatg cgcgtaataa caaggcagca agtacgccat tatgtccagc tcgggtgaca
     1561 tcaaggcagt aaaatgccag atcgttttcc gaaagtgcgg caatatcaag caccaggccg
     1621 ggtttgtccg cggcaaccaa ttgacgataa ttaacccggc aatgggaaat cacctgctga
     1681 accggcggtt gcaattcctg taataatttc gccgctgcgg cagggttatc aaccattgca
     1741 tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcggg aattaaacat gctgggatag
     1801 agacaggcaa aaaccatctc gcgcaagcga ttcagatctt taactggttt cagcaaaaca
     1861 tcttcaacgc ccagacgtaa cgctttggca atatctgcca tattttcagt ggcagatatc
     1921 accagaactg gggtctggtc gcctctgtta cgtatatgct ccagcagttt aagcccgttc
     1981 attcgtggca tcgcgatatc acatatcatc aggtctggag tgaaacctcc cagcaactca
     2041 agggcatcca ccccatcagc cgccagtacc gttgtcgctc ccaatgagga aaaccacgaa
     2101 tccagaagcg agcgaaatac ctgctcatct tcaacaatga gaatctgttt tccgaccaat
     2161 ggctgcgtca tgttctctcc cctgactggc tttactcaat agtggcatgc tattgccacc
     2221 tgcgcctgtc agaattaact taagtgtaga taaaaattct ggtcaaatgt tggtgcgtac
     2281 caacggcaaa agttcgtcca ttttcttttc cactgccagc tgcccggccg caatggcagc
     2341 gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa acaggttgaa tcagaatatc
     2401 gggcggatca cctgccatgc ggttcctttt aaggcggttc tccagcacct ggatagaagt
     2461 ggtcataatc tctgttgccg ttggcgctgt caccgcccga cgtgtcgtta tgctgcctaa
     2521 cctttctttc agacgcgcat gccacggcag agaatcacca ttctcgctat tttcttcact
     2581 gacattaaag gagagcaaat cttgttgcat caaatgagca tcgtgctgca ggtcgaccgc
     2641 tatcacaata tcagccccca atgcacgcgt gagggaaata ggaattgggt taactacagc
     2701 gccatcaacc agccagtagc cgttatgcgc aacgggtgcc attagtcctg gaatactgca
     2761 tgatgcacga atagcaagat ggagatcgcc ttcagtaaac cataattcgc gcccagtact
     2821 taaattggtg gcaacagccg cgaagcgacg ggaacaattt tcgatctctg tttccggcat
     2881 tatttcgcga tattgattga agacacgctc gccgcgcagt aacccaccgc gctgccagga
     2941 gagatccatc aggcgtaaaa catcccaata actgaaagag gtcacccaat cttccagcgc
     3001 agatagtcga tcgcatgcat aggcagcgcc caccagcgaa ccaattgaac atcctgcaac
     3061 gatatcaatt tcaataccca ctttttttag cgcattaata acgccaatat gcgaccaacc
     3121 tctcgccgcg ccagatccca gcgccagccc tatttttatc tttctcatta ttcccgcaag
     3181 attcccctgg aatgcaatcg tagcaacatc gctactgctc ggttaacata gtgctactca
     3241 ggcggttaat acgccgttat tgtttccagg gagattattt gtgtctcagc tttgtccctg
     3301 tggtagtgct gtcgagtata gcctatgttg ccacccttat gtgtctggtg aaaaggttgc
     3361 acctgatcca gaacatctca tgcgttcgcg ttactgcgct tttgtgatga aagacgcaga
     3421 ttatttaata aagacctggc atccatcttg tggagcggcg acgttacgtg ccgaattgat
     3481 tgccggattt gcgcatactg aatggttggg attgaccgta tttgaacatt gttggcagga
     3541 cgtcgacaat atcggatttg tcagttttgt tgcgcgattt accgaaggta gcaaaacagg
     3601 tgcaataatt gaacgttcgc gatttttgaa agaaaacggt caatggtact atattgacgg
     3661 tacacgtccg cagtttggcc gcaacgatcc ctgcccttgt ggttcaggta aaaaatttaa
     3721 aaagtgctgc ggccaataat ggttgacggt acggtttagc aaacactctc aacaaggttt
     3781 ttccagcaat gcattcactc caacgtaaag ttctgcgtac tatttgtccg gaccaaaaag
     3841 gtctgatcgc acgtattacc aatatttgct acaagcacga gttaaatatc gtacagaaca
     3901 atgaatttgt tgatcaccgt accgggcgct tttttatgcg cacggaactg gaaggaattt
     3961 ttaatgattc caccctgctg gcggatctcg acagcgcatt gccagaaggc tccgtacgtg
     4021 agctgaatcc tgccggtcgt cgccggatag tgattctggt cactaaagag gcgcattgcc
     4081 ttggcgattt gttgatgaaa gccaattacg gcggcctgga tgtcgaaatc gcggcagtta
     4141 ttggtaacca cgatacttta cgttctctgg ttgagcgttt tgatattccg tttgagctgg
     4201 taagccatga agggttaagc cgcaacgagc acgatcaaaa gatggcggat gccattgatg
     4261 cttatcagcc tgactacgtg gtgctggcga agtatatgcg ggtattaacg ccagaatttg
     4321 tggcacgctt cccgaataag atcatcaata ttcaccattc attcctgcca gcgtttatcg
     4381 gcgcacgtcc ttatcaccag gcctatgaac gtggcgtgaa gattattggc gctaccgctc
     4441 actatgtgaa tgacaatctg gacgaaggtc caatcatcat gcaggacgtt attcatgtcg
     4501 atcataccta cacagctgaa gatatgatgc gcgcaggtcg tgacgtcgag aaaaacgtct
     4561 taagtcgcgc actctacaaa gtactggcgc agcgcgtctt tgtttacggt aatcggacga
     4621 ttattcttta atcgccagca aaaataactg gttaccttta atccgttacg ggtgaaaatt
     4681 acgcaaccag ttcatttttc tcaacgtaac actttacagc ggcgcgtcat ttgatatgat
     4741 gcgccccgct tcccgataag ggagcaggct agtaaaaagc attaccccgt ggtggggttc
     4801 ccgagcggcc aaagggagca gactgtaaat ctgccgtcat cgacttcgaa ggttcgaatc
     4861 cttcccccac caccatcact ttcaaaagtc cgtgaactct caaacgaatc cgcgattaaa
     4921 tattctgccc aagcggggaa ggatgagaag cttcgaccag ggttcgactc gagcgccagc
     4981 gagagagcgt ggccgcaggc aacgacccga agggcgaagc gcgcagcgct gagtaatcct
     5041 tcctccacca ccatcacttc ctgtaaatac tcaaaacctg ttactccgag ctaattctgc
     5101 ccaatggcac cgcgtttacc ggacctacaa tatcctcccc tgtaggtcgg ataagcgaag
     5161 cgcatccgac aactcacccc gtaccgtgcc cataaaaaaa ccccgccgaa gcggggtcta
     5221 tgcaaactaa aattcgctta gtgacgagcg cgcaccagct gatacttacg tgtcagatac
     5281 tccacctgta cgctccagat gtgcaccaga cgcgagaacg ggaacagcag gaataacgtc
     5341 atccccaaca ccaggtgcag acggaagata aacgccacac catcaaggtg ttgagaagcg
     5401 ccaccgtgga aggtcactac cgactgcgcc cagccaacca gtttcatcat ctcgctaccg
     5461 tccatatgtt gggcggagaa cggaatggtc aacaggccca gcgcgcactg gataacgagc
     5521 agcgacagga tcaggatatc cgctccggta gtggttgcac gcacgcgtgg gctgaacaga
     5581 cgacgcttca gcaacagcac gccgccaatc agacacagca caccgctggc accaccagca
     5641 aacatcgcca ttttctgttt cacttcaatc ggcagccagg cttcatacat ccagtgcggc
     5701 gtcagcatac cgaagaagtg accaacaaaa atccccagaa taccgatatg gaacaggttc
     5761 gacgccaggt tcatcccttt gcgatccagc atctggctgg acgccgcgcg ccaggtgtac
     5821 tgcccgtagt cataacgcag ccagctacca atcaggaaga ccgccccggc tatgtacggg
     5881 tagatatcaa agaagaacat attcaggaat tgcattagtg ctgtcctccg gtggtgatat
     5941 tcagatattg cggcgcgacg gcaccggcaa agcgacgctg atgagcagtg attgctgaat
     6001 caccgcagcc tttttcagca aagaatttaa cctgctcttc ttcccagact gcatccagcg
     6061 cctgcggcgt atcgtcgcgc gcttcatcgg caattttttc cgccactttg tcgctgtcga
     6121 tggcggtatt cgccagtttc agcagcagat caaacagcac ggcataacgg ctttcacgct
     6181 gttgcagacg cgcgctcagc aatgccagaa tcggcgcaat atctttcaaa ccctccacgg
     6241 cttcgctttg cggcagctgc gccaggtact ccagatacag cggcagatgg tcgggcaatt
     6301 cgcggctgtt taactgcaag ccgtgctgct cgtactgcgc cagcagatcc accatcgcct
     6361 ggccgcggtc acgggattca ccgtgaacat gttcaaatag caacagtgac gtggcgcggc
     6421 cacggtcgaa cagttcgctg tactgcgcct gggcatcgag cggatccatc gtcgttaaat
     6481 cgcgcaggaa aatgcccagc gcatgggcat cctcttttgg cagatttttc gacgcggcaa
     6541 tcgcctcaaa catctcttgt tgatgctgcc ataaggcagc atccggatat tcaaggagac
     6601 gcgatacaat cacgagttcg atcatgggtg cggctccgtt ttactggtca cgtcgatggc
     6661 atcgatacga cggctgttaa acagattgaa tttggtatct gagccgtggc agccatcacc
     6721 aaaggtaaag ccgcagccat ttttctccgg gaaggcttcc cgcgccagtt cacgatgact
     6781 gctcggcacc acaaagcgat cttcgtagtt agcaatcgcc agataacggt acatctcctg
     6841 tgcctgggct tcggtcagac cgacctcttc cagcgcacgg gtatcaactt taccgtcaac
     6901 ggtttcagca cgtttataat gacgcatcgc cagcatacgt ttcagcgcgc gcagtaccgg
     6961 tttggtatca cctgcggtca gcagattcgc cagatactgt accggaatac gcaggctttc
     7021 gacgtctggc agaatgccat tgctgcccag ctcacccgca tctgccgcag actgaatcgg
     7081 cgacagaggt ggcacatacc agaccatcgg cagcgtgcga tattccggat gcagcggcag
     7141 cgccagtttc cattccatcg ccattttata aaccggcgac tgctgtgcgg cttcaataac
     7201 gctcagcgga ataccgtctt taatcgcctg ctcgatgacc ttcggatcgt tcggatcgag
     7261 gaacacgtcc agctgacgct ggtagagatc tttctcgttc tcggtgctgg ctgcacgttc
     7321 aatcgcgtcg gcatcgtaca gcagcacgcc aagataacgg atacgaccga cacaggtttc
     7381 agagcacacc gtcggctgac cggcttcaat acgcggatag cagaagatgc acttctcaga
     7441 cttaccgctc ttccagttga agtagatttt tttgtacggg catccggtga tgcacatacg
     7501 ccagccacgg catttgtcct ggtcgatcag gacgatgcca tcttcttcac gcttgtaaat
     7561 cgcaccgctc gggcaggtcg ccacacatgc cgggttcagg cagtgttcgc acaggcgtgg
     7621 caaatacatc atgaaggtgt tttcgaactg gctatacatc gccttctgga tgttgtcgaa
     7681 gttcttgtct ttcgccagtt tgtcaaactc gccgcccagg tcatcttccc agtttggccc
     7741 tttttcgatt ttcgccatcc gttcgccggt aatcagcgaa cgtggacggg caatcggctg
     7801 cgatttgctg ccttccggcg cggtatgcag gttctgatag tcaaaatcga acggctcgta
     7861 ataatcgtcg atccccggca gatgcgggtt agcgaaaatt ttacccagca gcatggcgcg
     7921 gttacccatg cgcggttgca gtttgccgtt gattttacgg atccagccgc ctttgtattt
     7981 ttcctggttt tcccagtctg tcgggaagcc ctggcccggc ttggtttcca cgttgttgaa
     8041 ccacgcgtat tccacgcctt cacggctggt ccagacgttt ttacaggtca ctgaacaggt
     8101 gtggcaaccg atgcacttat ccagattcag caccatgccg acttgtgaac gaattttcat
     8161 tttacgctct cctgtacctg gtcattgcct tcgccatcta accagtcaat gttcttcatc
     8221 ttacgcacta caacaaattc atcgcggtta gaacctacag tgccgtagta gttaaagccg
     8281 tatgccagat gggcatagcc gccgatcata tgcgtcggtt tcggcgtgat acgggtgacc
     8341 gagttatgaa taccaccgcg ctggctggtg atttctgaac ccggcaggtt aacgatacgt
     8401 tcctgcgcgt ggtacatcat ggtcatccct gccggaacac gctggctgac aaccgcacgg
     8461 gcagtcaatg caccgttgct gttgaagact tcaatccagt cgttgtcggc gatacccagc
     8521 tctttggcat cggcttcact caaccagacc accggaccac cgcgacctaa agtcagcatc
     8581 agcaggttgt cgctgtaggt ggagtggata ccccacttct ggtgcggtgt caggaagttg
     8641 agcgcttttt ccggattacc gttagatttc tggcccatca cttctttcac cgaacgggtg
     8701 tcgatcggcg gacgataaac cagcaggctt tcaccgaaat cacgcatcca ctgatgatcc
     8761 tgatacagct gctgacggcc agagagcgta cgccacggga tcagctcgtg aacgttggtg
     8821 taaccggcgt tgtaagaaac atgttcatct tcaaggccag accaggtcgg gctggagatg
     8881 attttgcgcg gctgcgcctg aatgtcacgg aagcggatct tctcgtcttc tttattcagc
     8941 gccagatgcg tatggtcacg accggtaaac tcactcaatg ccgcccaggc tttcacggcc
     9001 acctggccgt tggtttctgg tgccagcgtc aggatcatct ctgctgcatc aattgcggtg
     9061 ttgagcatcg gctggccttt cgccggacct tccgctttgg tgtagttgag cttacgcagc
     9121 agatccatct cgctctgggt gttccaggca atccctttac cgccgttacc gattttctcc
     9181 atcagcgggc cgatagaggt aaagcgttcg taagttgccg gataatcgcg ctctacaacc
     9241 ataatgtgcg gtgcggtttt acctgggatc aggtcacatt cgcctttttt ccagtctttc
     9301 acatccagcg cttgcgccag ttcagcggca gagtcgtgct ggataggcag cgtgacgatg
     9361 tcggtttctt tgcccagatg accaacgcac acttcggaga atttcttcgc gatggctttg
     9421 tagatttccc agtcgctttt cgcttcccag gccggatcga ccgcagcaga cagcgggtga
     9481 ataaacggat gcatatccga agtattcatg tcgtcttttt cgtaccaggt cgccgtcggc
     9541 aaaatgatgt cggaatagag acaggtgctc gacagacgga agtccagcgt caccaccaga
     9601 tccaacttgc cttccagacc gttgtcctgc cagtccactt cttccggctt cacgccgccc
     9661 tgctgaccca ggtctttacc ctggataccg tgctccgtcc ccagcaggta cttgagcata
     9721 aactcatgac ctttaccgga agaaccgagc aggttagaac gccagatgaa caggttacgc
     9781 gggtggtttt taccgttttc tggttgttct gccgcaaaac ggatggaacc ctctttcagg
     9841 gatttcaccg tatagtccac cggattcatc ccggcttttt ctgcttcgcg agcgatagtc
     9901 agcgggttag tgcctaactg cggcgcagac ggcagccagc ccatacgttc tgcacggacg
     9961 ttaaagtcga tcagatgtcc ggtataacgg gatttatccg ccatcggcga caacaactct
    10021 tctgcggtca cggtttcata acgccactgg ctggagtggt tatagaaata agaggtgctg
    10081 ttcatgtgac gcgccggacg ctgccagtca agagcaaacg ccagcggctg ccagccggtt
    10141 tgcggacgca gtttttcctg acctacatag tgcgcccagc caccaccgct ctgacctaca
    10201 cagccgcaga aaatcagcat gttgatcaga ccacgatagt tcatatcgag gtgataccag
    10261 tggttcagcc ccgcaccgac gataatcatc gaacgaccgt gcgtcttgtc agcgttatcg
    10321 gcaaattcac gggcaatgcg gataatctgg ctgcgagaaa cgccagtgat ctgctcggcc
    10381 catgcagggg tgtaggcttt cacgtcgtca tagctggttg cgcagttaac gtcgttcagg
    10441 ccacgttcca gaccgtagtt tgccaacgtc agatcataaa cggtggtcac cagagcagtg
    10501 ctgccatcag ccagttgcag gcgtttcacc ggcagcttgt gcagcagcac gttttccagt
    10561 tccactttgt tgaagtgttc cgtgccgtcg ccaccaaagt atgggaagcc cacttcagcg
    10621 atctcatcct ggctacccat caggctcagt tgcagctcgg tttcttcgcc agttttgccg
    10681 tcgcgctgct caagattcca tttgcccttc tcgccccagc ggaagccaat agaaccgttc
    10741 ggcgcaacca tttctccatt ggtattaaag gcgacagttt tccattccgg attgttttcc
    10801 tggcccagcg catccaccag atcagcagcg cgcagcatac gacccgcagc gtagtaaccg
    10861 tcgcgctctt ccagcatcac cagcattggc atgtcggtat aacgacgcac atagtcggtg
    10921 aaatactggc ttgggttgtc gaggtggaat tcacgcagca tcacgtgacc catcgccagc
    10981 gccattgctg cgtcggtgcc ctgcttcggt gccagccaca gatcgcacag tttggcgatt
    11041 tcagcgtagt ccggcgtgac ggcaacggtt ttggtgcctt tgtaacgcac ttcagtaaag
    11101 aagtgagcat ccggagtacg cgtctgcggc acgtttgacc cccaggcgat gatgtagctg
    11161 gagttgtacc agtcagcaga ttccggtacg tcagtttgct cgccccaggt ttgtggagac
    11221 gcaggaggca gatcgcagta ccagtcgtag aagcttaagc aagtaccgcc aagcagcgag
    11281 agataacgcg cacccgacgc gtaagaaacc atcgacattg ccggaattgg cgagaagcca
    11341 gcgacacggt ccgggccgta gtttttgatg gtgtaaacgt tagatgcggc gatcagttcg
    11401 ttcacttcct gccaggaaga acgaacaaaa ccaccgcgtc cacgcgcctg cttaaagctt
    11461 ttcgctttat cggcgtcttc aatgatagaa gcccatgcct caaccggatc gctatgcagc
    11521 gccttcgctt cacgccacat tttcatcagg cgtttgcgca tcatcgggta tttcagacgg
    11581 ttggcgctgt aaagatacca ggagtagctg gcaccgcgcg ggcagccgcg aggttcatgg
    11641 tttggcagat ccgggcgagt acgcggatag tcagtctgct gggtttccca ggtgaccaga
    11701 ccgtttttaa cgtagatttt ccagctgcag gagccggtgc agtttacccc atgggtagag
    11761 cggacgattt tgtcatgctg ccaacgctgg cgatatccat cctcccagtc acggttggta
    11821 ttgagaagct ggccatgccc atcggcaaag gtttcgccct tctgtttgaa gtagcgaaac
    11881 cggtccagga atttactcat cggttttctc ctgtgggagc ctgtcggctc tctgataaat
    11941 cgacattgct aatgtttaag ggcaaggtaa cgctctgaaa cagcgtggga attgataacg
    12001 atcaagagtg atggggatga aaaataaagt aaattcctaa gat
//
LOCUS       DAZKGF010000145        11998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZKGF010000145 DAZKGF010000000
VERSION     DAZKGF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.145"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2114
                     /locus_tag="ACHOQV_003857"
     CDS             <1..2114
                     /locus_tag="ACHOQV_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFP9632151.1"
                     /translation="CGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYREL
                     NKAAQRSITAPDRIGEGKTWVYQRDTMVERGDNRGVHQYGPAEHFTHIISDKPSPKDK
                     YVAYAINIPDYELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQC
                     EIITPKKPGEAILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEK
                     GYWFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDL
                     DYPSADIRAKISKDIESGIPGYWQGQVLIPFTIDPVTKERKQIHPEDIHLTAKDFETS
                     KDNISKDEWENLHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAK
                     HSKHEQIERSITPPLTIYNERLHNTNLFKPIYHEKAVMNYMEYLVTCQLDEEQMSSLL
                     YERDDLSAIDFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSKRRILININHDFPNN
                     GNLFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEA
                     WLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGL
                     VIINKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKAFMLRGGTLNIN
                     IPGRSALLLGKTGEPPNYLYL"
     gene            2451..3428
                     /gene="glaH"
                     /locus_tag="ACHOQV_003858"
     CDS             2451..3428
                     /gene="glaH"
                     /locus_tag="ACHOQV_003858"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFP9632152.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATNHYQTHQ"
     gene            3448..4716
                     /gene="lhgO"
                     /locus_tag="ACHOQV_003859"
     CDS             3448..4716
                     /gene="lhgO"
                     /locus_tag="ACHOQV_003859"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFP9632153.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALQEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4739..6187
                     /gene="gabD"
                     /locus_tag="ACHOQV_003860"
     CDS             4739..6187
                     /gene="gabD"
                     /locus_tag="ACHOQV_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9632154.1"
                     /translation="MKPNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6201..7481
                     /gene="gabT"
                     /locus_tag="ACHOQV_003861"
     CDS             6201..7481
                     /gene="gabT"
                     /locus_tag="ACHOQV_003861"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFP9632155.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYI
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGHKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            7719..9119
                     /gene="gabP"
                     /locus_tag="ACHOQV_003862"
     CDS             7719..9119
                     /gene="gabP"
                     /locus_tag="ACHOQV_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFP9632156.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9140..9802
                     /gene="csiR"
                     /locus_tag="ACHOQV_003863"
     CDS             9140..9802
                     /gene="csiR"
                     /locus_tag="ACHOQV_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFP9632157.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKRDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9803..10252)
                     /gene="kbp"
                     /locus_tag="ACHOQV_003864"
     CDS             complement(9803..10252)
                     /gene="kbp"
                     /locus_tag="ACHOQV_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFP9632158.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPAAASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10336..10494)
                     /gene="yqaE"
                     /locus_tag="ACHOQV_003865"
     CDS             complement(10336..10494)
                     /gene="yqaE"
                     /locus_tag="ACHOQV_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP9632159.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10677..10976
                     /gene="ygaV"
                     /locus_tag="ACHOQV_003866"
     CDS             10677..10976
                     /gene="ygaV"
                     /locus_tag="ACHOQV_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9632160.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10986..11510
                     /gene="ygaP"
                     /locus_tag="ACHOQV_003867"
     CDS             10986..11510
                     /gene="ygaP"
                     /locus_tag="ACHOQV_003867"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFP9632161.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11557..11961)
                     /gene="stpA"
                     /locus_tag="ACHOQV_003868"
     CDS             complement(11557..11961)
                     /gene="stpA"
                     /locus_tag="ACHOQV_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFP9632162.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         3009 a         2955 c         3094 g         2940 t
ORIGIN      
        1 cttgtggagt ttatcctcag gatttaaaag acagagaaat tatatcaact tttcacgcag
       61 aaaaagtcat taatgatctg ttaaaaaacg attataaaat ttccctttct ccagatacaa
      121 cttaccgaga gctgaataaa gctgcacagc gtagcattac agcgccagac aggataggag
      181 aagggaaaac atgggtttat caacgagata caatggttga aagaggtgat aacagaggcg
      241 ttcatcagta tggtccagct gaacatttca ctcacattat atctgacaaa ccatccccaa
      301 aagataagta tgttgcatat gctattaaca ttcctgacta tgagctagca gccgatgtat
      361 ataatattaa cgtgacgtca ccttccggac agcaagaaac atttaaaata ctaatcaatc
      421 cagaacatct acggcaaaca cttgagcgta aatctcttac tgctgttcag aaatcacaat
      481 gtgaaatcat cacccccaaa aaacctggcg aagcgattct tcatgctttt aatgccacct
      541 accagcaaat cagggaaaat atgtctgagt ttgcacgttc ccattatggg tatatacaaa
      601 ttcctccagt gacaactttc cgcgccgacg gaccagaaac tcccgaagaa gaaaaaggtt
      661 actggtttca cgcttatcaa cccgaagatc tttgtaccat ccataatcca atgggagatt
      721 tgcaggattt tatcgcattg gttaaagatg ctaaaaaatt tggtatcgat atcattcctg
      781 attatacctt taactttatg ggaattgggg gtagtggtaa aaatgaccta gattatccct
      841 ctgctgatat acgagcgaag atcagtaaag atatagaaag tggtatccct ggctattggc
      901 aaggtcaggt tttgattcca tttactatag atccagtaac aaaagaacgt aaacaaatcc
      961 atccagaaga tatacatctc actgcaaaag actttgaaac aagtaaagat aacatctcta
     1021 aggatgaatg ggaaaacctc catgcattaa aagaaaagcg tttaaatgga atgcctaaaa
     1081 caacccccaa aagtgaccag gttattatgt tgcaaaatca atacgttcgt gaaatgcgaa
     1141 aatatggcgt acgaggttta cgttacgatg cagcaaaaca ctcaaaacat gaacaaatag
     1201 aaagatcaat aactccaccg cttacaattt ataatgagcg attacacaat actaacttat
     1261 ttaagccaat atatcatgaa aaagccgtta tgaattacat ggaatacctg gtaacttgtc
     1321 agttggatga agaacaaatg tcatcgctgc tttatgaaag agatgattta agcgccattg
     1381 atttttcatt gctcatgaag acgataaaag ccttttcatt tggtggcgat ctccaaaccc
     1441 ttgcatcaaa accgggttca acaatctcaa gcatcccgtc aaaaagacgg atattgatta
     1501 acattaacca cgattttcct aacaatggca atcttttcaa tgactttcta tttaaccatc
     1561 aacaagatga acaattagca atggcatata tggccgctct cccgttcagc aggcctttag
     1621 tttactggga tggccaagta ttaaaatcaa cgactgaaat taaaaattat gatgggtcga
     1681 cgcgtgtcgg cggtgaggcg tggcttaata aaggttgctc tacctatcag cagctctaca
     1741 atgaattcca cgcattatat atagataaag caggaatatg gagcgcattt gagggtgtat
     1801 ttgcaactaa aaacgttctg gcctttagtc gtggggattc tgtgaacatt aatcactctc
     1861 ctcatgatgg actagttata ataaataaag gaaacgaaga agttgaaggt acctggccta
     1921 acaaattgca acctggaata tataaaaaca tggggagtaa tagcgttaac attattatta
     1981 ataatacccg aaaaattatc ccccctggta aagcatttat gcttagaggc ggaactctaa
     2041 atatcaatat tcctgggcgt agcgctcttc ttttagggaa aactggagaa ccgccgaact
     2101 atctctattt gtaatttaat gttatatctg ccccgctaaa acggggcaga taatatgttt
     2161 agtttactaa cggtcatttt gcagtgaagc catttaccgt tttttatcga ccagataatc
     2221 tgttctctga tggtaatcca cccctaacct gttgctttag ttattcattt cctgtctcac
     2281 tttgccttaa taacctacgt caaatgttac caatttgttg tttttgatcg caataagaaa
     2341 acaatatgta acttttatgc gcatttttca gaaatgtaga tatttttaga ttatggctac
     2401 gaaatgagca tcgccatgtc accctacatc tcataagagg atcgcttctg atgaatgcac
     2461 tgaccgccgt acaaaataac gctgtcgatt caggccagga ctatagcgga ttcactctca
     2521 tcccgtcggc gcaatctccg cgtctgctgg aactcacctt caccgaacag acgaccaaac
     2581 agtttctcga acaggttgcc gaatggcccg tgcaggcgct ggagtacaaa tcttttctgc
     2641 gttttcgggt aggcaaaatt cttgacgatc tgtgtgcgaa ccagctgcaa ccattgctgt
     2701 tgaagaccct gctaaaccgt gctgaaggtg cgctgttgat caatgcggtg ggtgtcgatg
     2761 atgtgaagca agcggatgag atggtgaagc tggcaacggc ggtagcgcat ctgattggtc
     2821 gctcaaattt cgatgcgatg agcggtcagt attacgcgcg attcgtggtg aaaaatgttg
     2881 ataactcaga cagctatctg cgtcagccgc accgcgtaat ggagctgcac aacgacggca
     2941 cctacgtcga agagatcacc gactatgtgc tgatgatgaa aatcgacgag cagaacatgc
     3001 agggtggaaa ttcgttgctg ctgcatctcg atgactggga acatctggac cactatttcc
     3061 gccacccgct ggcgcgtcgc ccgatgcgct ttgccgcgcc tccgagcaaa aacgtcagca
     3121 aagatgtttt ccatccagtg ttcgacgttg atcaacaggg tcgcccggtg atgcgttata
     3181 tcgaccagtt cgtccagcca aaagacttcg aagaaggcgt gtggctgagt gagctttccg
     3241 atgccattga aaccagcaaa ggcattcttt ctgtgcccgt tcccgttggc aaattcctgt
     3301 tgattaacaa tctattctgg ctgcacggtc gcgaccgctt tactccgcac ccggatctgc
     3361 gccgtgaact gatgcgtcag cgtggctatt tcgcttacgc cactaaccac taccagacgc
     3421 atcagtaagc gcaaaggaat tgagcggatg tatgattttg tgattattgg cggcggcatc
     3481 atcggcatgt cgaccgccat gcaactgatt gatgtttatc cggatgcccg cattgcgttg
     3541 caggaaaaag agtccggccc ggcctgtcac cagacgggcc acaacagtgg cgtgatccat
     3601 gccggggtct attacacgcc cggtagcctg aaggcacagt tttgcctggc gggaaaccgc
     3661 gccaccaaag ccttttgcga tcaaaacggc attcgctatg acaactgcgg caagatgctg
     3721 gtcgccacct ctgaactcga aatggaacgg atgcgcgcgt tgtgggaacg cacggcagcg
     3781 aacggtatcg agcgcgagtg gttaaacgcc gaggaactgc gcgagcgcga accgaatatc
     3841 accgggctcg gcggcatttt tgtgccgtcc agcggcattg tcagctaccg cgaagtgacg
     3901 gcggcgatgg caaaaatctt ccaggccaga ggcggcgaga ttatttataa cgccgaagtc
     3961 agcgccctca gtgagcataa aaacggcgtg gtgatacgta cccgtcaggg cggtgaatat
     4021 gaagcatcga cgctgattag ctgttccggg ctgatggctg accggctggt gaaaatgctc
     4081 ggcctcgaac cgggctttat tatctgcccg ttccgtggcg agtatttccg ccttgcgccg
     4141 gagcataacc agattgttaa ccacctgatt taccccattc ccgaccccgc gatgccattt
     4201 ttgggcgtgc atctcacccg catgatcgat ggcagcgtga ccgtcgggcc aaacgcggtg
     4261 ctggctttca aacgcgaagg ctatcgtaag cgcgacttct catttagcga cacgctggag
     4321 attttgggct cgtcggggat tcgccgggtg ctgcaaaacc atctacgctc aggactgggc
     4381 gagatgaaaa actcgctgtg caaaagcggc tatctgcggc tggtgcaaaa gtattgtccc
     4441 cggctttcgt taagcgatct ccagccctgg cccgctggtg tgcgggcgca ggcggtatcg
     4501 ccggacggca agctgattga cgattttctg tttgtcacca ccccgcgcac gatccacacc
     4561 tgcaatgcgc cctccccggc agcgacatca gcaattccta ttggtgcgca tattgtcagc
     4621 aaggtacaaa cgctgttggc aagccagagt aaccccggac gcacgctgcg agcggcacgt
     4681 agtgtggatg ccttacacgc cgcatttaat caataacctt tgaaaacagg acgtaacaat
     4741 gaaacctaac gacagtaact tattccgcca gcaggcgttg attaacgggg aatggctgga
     4801 cgccaacaat ggcgaggtca tcgacgtcac caatccggcg aacggcgaca agctgggtag
     4861 cgtacccaaa atgggcgctg atgaaacccg cgccgctatc gacgccgcca accgcgctct
     4921 gcccgcctgg cgtgcgctca ccgccaaaga acgcgccacc attctgcgca actggttcaa
     4981 tttgatgatg gagcatcagg acgatttagc gcgcctgatg accctagaac agggcaaacc
     5041 gctggccgaa gcgaaaggcg aaattagcta cgccgcctcc tttattgagt ggtttgccga
     5101 agaaggcaaa cgcatttatg gcgacaccat tcctggtcat caggccgata aacgcctgat
     5161 tgttatcaag cagccgattg gcgtcaccgc ggctatcacg ccgtggaact tcccggcggc
     5221 gatgattacc cgcaaagccg gtccggcgct ggcggcaggc tgcaccatgg tgctgaagcc
     5281 cgccagtcag acgccgttct ctgcgctggc gctggcggag ctggcgattc gcgcaggcgt
     5341 tccggctggc gtatttaacg tggtcaccgg ttcggcgggc gcggtgggta acgaactgac
     5401 cagcaatccg ctggtgcgca agctgtcgtt taccggctcg accgaaattg gccgccaatt
     5461 aatggaacag tgcgcgaaag acatcaagaa agtgtcgctg gaactgggcg gcaacgcgcc
     5521 gtttatcgtc tttgacgatg ccgacctcga caaagccgtg gaaggcgcac tggcctcgaa
     5581 attccgcaac gccgggcaaa cctgcgtctg cgccaaccgt ttatacgtgc aggacggcgt
     5641 gtatgaccgc tttgccgaaa aattacagca ggcggtgagc aaactgcaca tcggcgacgg
     5701 gctggagaat ggcgtcacca tcgggccgct gattgatgaa aaagcggtag caaaagtgga
     5761 agagcatatt gccgatgcgc tggagaaagg tgcgcgtgtg gtttgcggcg gtaaagcgga
     5821 tgaacgcggc ggcaacttct tccagccgac cattctggtg gacgttccgg ccaacgccaa
     5881 agtgtcgaaa gaagagacgt tcggccccct tgccccgcta ttccgcttta aagatgaagc
     5941 cgatgtgatt gcgcaggcca atgacaccga gtttggcctg gccgcctatt tctacgcccg
     6001 tgatttaagc cgcgtcttcc gcgtgggcga ggcgctggag tacggcatcg tcggcattaa
     6061 tactggcatt atttccaatg aagtggcccc gttcggtggc attaaagcct cgggtctggg
     6121 tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa atcaaatata tgtgcatcgg
     6181 tctttaactg gagaacgcga atgagcagca ataaagagtt aatgcagcgc cgcagtcagg
     6241 cgattccccg tggcgttggg caaattcacc ccattttcgc tgaccgcgcg gaaaactgcc
     6301 gggtgtggga cgttgaaggc cgtgagtata ttgatttcgc gggcggcatt gcggtgctca
     6361 ataccgggca cctgcatccg aaagtggttg cggcggtgga agcgcagttg aaaaagctgt
     6421 cgcacacctg cttccaggtg ctggcttacg aaccgtatct ggagctgtgc gagattatga
     6481 atcagaaggt gccgggagat ttcgccaaaa aaacgctgct ggtaacgacc ggttctgaag
     6541 cggtggagaa cgcggtgaaa atcgcccgcg ccgccaccaa acgcagcggg accatcgcct
     6601 ttagcggcgc gtatcacggg cgcacgcatt acacgctggc gctgaccggc aaggtgaatc
     6661 cgtactctgc gggcatggga ctgatgcctg ggcacgttta tcgcgcgctt tatccttgcc
     6721 cgctgcacgg catcagtgaa gatgacgcta tcgccagcat tcaccgaatt tttaaaaatg
     6781 acgctgcgcc ggaagatatc gccgccatcg tgattgagcc ggttcagggc gaaggcggtt
     6841 tctacgccgc gacgccagcc tttatgcagc gtttacgcgc gctgtgtgac gagcacggga
     6901 tcatgctgat tgccgatgaa gtgcagagcg gcgcggggcg taccggcacg ctgtttgcga
     6961 tggagcagat gggcgttgcg ccggatctta ccacctttgc gaaatcgatc gcgggcggct
     7021 tcccgctggc gggcgtcacc gggcgcgcgg aagtgatgga tgccgtcgct ccaggcggtc
     7081 tgggcggcac ctatgccggt aacccgattg cctgcgtggc agcgctggaa gtgttaaagg
     7141 tattcgagca ggaaaatctg ctgcaaaaag ccaacgacct ggggcataag ctgaaagacg
     7201 gattgctggc gatagccgaa aaacacccgg agatcggcga tgtacgcggg ctgggagcga
     7261 tgattgccat cgagctgttt gaagatggcg atcacagcaa accggacgcc aaactcaccg
     7321 ccgagatcgt ggcgcgtgcc cgcgataaag gcctgattct tctctcctgc ggcccgtatt
     7381 acaacgtgct gcgcatcctt gtaccgctca ccattgaaga cgctcagatc cgtcagggtc
     7441 tggaaatcat cagccagtgt tttgatgagg cgaagcagta gatccgtacc catgccggag
     7501 gcgacgctgc gcgtcttgtc cggcctacgc gaatccaggt cggataaagc gtttacgctg
     7561 catccgacaa tctgtacgtg aacaggaata aatctatgtc ggccggataa ggcggagccg
     7621 ctctccggta aaaagaacca ataacaatta cacgcgtgac ccgacgcggg aaatgtcggg
     7681 gcgctctccc aagtgacaca ctttcgagag gattcaggat ggggcaatca tcgcaaccac
     7741 atgagttagg cggcgggctg aagtcacgcc acgtcaccat gttgtctatt gccggtgtta
     7801 tcggcgcaag tctgtttgtc ggttccagcg tcgccatcgc cgaagcgggc ccggcggtat
     7861 tactggccta tctgttcgcc ggattactgg tggttatgat tatgcggatg ttggcggaaa
     7921 tggcggttgc cacgcccgat accggttcgt tttccaccta tgccgacaag gcaattggcc
     7981 gctgggcggg ttataccatc ggctggctgt actggtggtt ttgggtactg gttatcccgc
     8041 tggaagccaa catcgccgcc atgatcctgc actcgtgggt tccaggcatt cccatctggt
     8101 tattttccct ggtcattacc ctcgccttga ctggcagtaa tttattaagc gttaaaaact
     8161 acggcgaatt tgagttctgg ctggcgctgt gcaaagtcat cgctatcctg gcctttattt
     8221 tccttggtgc agtcgcaatt agcggttttt acccttatgc cgaagtgagc gggatctcaa
     8281 gattgtggga tagcggcggc tttatgccca acggcttcgg cgcagtatta agcgcgatgt
     8341 tgatcaccat gttctcgttt atgggcgcag aaattgtcac cattgccgcc gcggaatctg
     8401 acacgccgga aaaacatatt gtccgcgcca ccaactcggt tatctggcgt atttctatct
     8461 tctatttatg ctctattttt gtcgtggtgg cattaatacc gtggaatatg ccggggctga
     8521 aagccgttgg atcttatcgc tcggttctgg agttgctcaa tattccccat gcgaaattaa
     8581 tcatggactg cgtgatatta ctttccgtaa ccagttgcct gaactcggcg ctgtataccg
     8641 cttccagaat gctctactcc ttaagccgtc gcggtgatgc tcccgcggta atgggtaaaa
     8701 tcaaccgcag taaaaccccg tatgtggcgg tattactctc gaccggtgcg gcatttttaa
     8761 cggtggtggt gaattattac gcgcctgcga aagtgtttaa atttctgatc gatagctccg
     8821 gcgctatcgc cctgctggtt tatttagtca ttgccgtttc acaattgcgg atgcgcaaaa
     8881 ttctgcgagc agaaggaagc gaaattcgcc tgcggatgtg gctttatccg tggctcacct
     8941 ggctggtcat cggttttata acctttgtgt tggtagtgat gctattccgc ccggcgcaac
     9001 agttagaagt gatttccacc ggtttattag cgatagggat aatctgtacc gtgccaatta
     9061 tggctcgctg gaaaaagctg gtattgtggc aaaaaacacc cgttcataat acgcgctaat
     9121 aatgatcagg agtcacacca tgaccattac gtctctggat ggctatcgct ggctgaagaa
     9181 cgatattatt cgcggaaact ttcaaccgga tgaaaaatta cggatgagtt tgctgacatc
     9241 gcgttatgca cttggcgttg ggccgttgcg ggaagcgctt tcgcaactgg tagcagaacg
     9301 actggtcacg gtggtgaatc aaaaagggta tcgggtggcg tctatgtcag agcaagagct
     9361 gctcgatatt ttcgacgccc gcgccaatat ggaagcgatg ctggtcagtc tggcgattgc
     9421 ccgcggtggc gatgaatggg aggcagacgt tctcgcaaaa gcgcatctgc tcagtaagct
     9481 ggaggcctgt gacgccagtg agaaaatgct tgatgagtgg gatctgcgtc atcaggcgtt
     9541 tcatactgca attgtggcgg gctgtggttc tcactatttg ctgcaaatgc gtgaacggtt
     9601 gtttgatctg gcggcgcgtt atcgatttat ctggctgcgg cgaacagtgc tttcggtgga
     9661 aatgctggaa gataaacgcg atcagcacca gaccctcact gcggcggtac tggcgcgaga
     9721 taccgcgcgc gccagtgagt taatgcgcca gcatttactg acgccaattc ccattatcca
     9781 gcaggcgatg gctggcaatt aactactctt caggaatacg caacacttgc cccgggtaaa
     9841 ttttatccgg gcttttgagc atcggtttat tcgcttcgaa gattttattg tacagattag
     9901 cgttaccgta gacctgtttg gaaatggcac tcagagtgtc gccagactta acggtataaa
     9961 actggctggc agcggctggt gtcgccgttt tcacctgatc atcgacactg gcaataccgg
    10021 aaatattccc caccgcaaca aggattttct ccttcgcctc ctgacttaag ccgtcacccg
    10081 tgaccgtcgc tttgccgtcg gcgatttgaa tgttcacttt atcggcatca ggtattccgg
    10141 ttttgttcag atgctcctgc accttcttcg cctgatcgtc tttatcgtgc tgacctgtaa
    10201 ccgcgtccca gagtttttct ccggcatctt tcacaaaatt gaacagaccc atagctacct
    10261 cattcgttaa cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt taacgccgcg
    10321 ggggtgaaca caggtttaat cgcgggtttg cacccagaac gcgtgaatca aaccggggat
    10381 atacccaagc agtgtcagca gaatattaat aatgaacgcc caaccgaacc ctttaccgag
    10441 cagcacgccg agcggcggca gaatgatggt gatgacgatt ctccagaaac ccatatgtac
    10501 tccctataag aaaattactc attgtttaaa aagagatttt atctcttaag cgtagtaatt
    10561 ttagcggagc ctgccagttt cagcttacgc ttttactctc atttacgctg ctgaggctgg
    10621 tatatgttgc atttttagtt tagagtttac ttaatttaga aaatacttaa atatctatga
    10681 ctgaactcgc gcaattacag gctagtgccg aacaggcagc ggccttattg aaagcaatga
    10741 gccaccctaa acggttgctg attctgtgca tgcttagcgg ttcccccggc accagtgcgg
    10801 gagagctgac gcgcattacc ggactgagtg cctctgcaac atcacagcat ctcgctcgta
    10861 tgcgggacga agggcttatc gacagccaac gggatgccca acgcattcta tattccatta
    10921 aaaatgaggc ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag
    10981 tcactatggc tttgacaacc atttcgccgc atgatgcaca agaattaatc gcacgcggcg
    11041 caaagttaat cgatattcgt gacgctgatg aatatcttcg tgaacatatt cctgaagcag
    11101 atctggctcc attatccgtg ctggaacagt caggtcttcc ggctaaatta cgtcgcgagc
    11161 aaataatatt ccactgccag gctggtaaac gcaccagtaa taacgccgat aaattagcag
    11221 cgattgccgc ccccgcagaa atctttttac tcgaagatgg gattgatggc tggaaaaaag
    11281 caggattacc agtagcggta aataaatctc aacccttgcc gttaatgcgc caggtgcaga
    11341 tcgctgcggg tggtttaata ttaatcggcg ttatactagg ttataccgta aatagcggtt
    11401 tcttcttatt aagcggcttt gttggtgccg ggttactgtt tgcaggaatt agcggttttt
    11461 gtgggatggc aaggttgtta gataagatgc cgtggaacca acgagcttga gaagcgacgc
    11521 cggacgcgcc atagcagcaa catccggcct cagtaattag atcagaaaat cgtcgagaga
    11581 tttaccttct gccagcgcct gagcgattgg cttcggtgta cggccctgac cggtccaggt
    11641 tttagtttca ccgttaacat cgatgaattt atatttcgcc ggacgcggct ggcgtttttt
    11701 accagcgcgt ggtgccgcag cagagctatt acccaataac tcttccgggt taattccgtc
    11761 agctttcatc agctccagcc aggtgctaat tttttcctgg cgctctgcca gttcacgctg
    11821 ctgttgttct tcttcttcac gtctttcttt agtgacaacc ctgaattttt cgagcatttc
    11881 ttcaagaacg tcaatggaga attcgcgagc catcgcacgg agggtgcgaa tgttatttaa
    11941 actttgtaac attacggaca taataaagaa aaccttttaa cgccaaaaca aaaaagta
//
LOCUS       DAZKGF010000146        11905 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZKGF010000146 DAZKGF010000000
VERSION     DAZKGF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.146"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            98..967
                     /locus_tag="ACHOQV_003869"
     CDS             98..967
                     /locus_tag="ACHOQV_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP9632163.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMVENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1017..1370)
                     /locus_tag="ACHOQV_003870"
     CDS             complement(1017..1370)
                     /locus_tag="ACHOQV_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP9632164.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1508..3154)
                     /gene="groL"
                     /locus_tag="ACHOQV_003871"
     CDS             complement(1508..3154)
                     /gene="groL"
                     /locus_tag="ACHOQV_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9632165.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3198..3491)
                     /locus_tag="ACHOQV_003872"
     CDS             complement(3198..3491)
                     /locus_tag="ACHOQV_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9632166.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3767..5023
                     /gene="yjeH"
                     /locus_tag="ACHOQV_003873"
     CDS             3767..5023
                     /gene="yjeH"
                     /locus_tag="ACHOQV_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP9632167.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLSRLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLTMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5039..5515)
                     /locus_tag="ACHOQV_003874"
     CDS             complement(5039..5515)
                     /locus_tag="ACHOQV_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP9632168.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5852..7288
                     /gene="aspA"
                     /locus_tag="ACHOQV_003875"
     CDS             5852..7288
                     /gene="aspA"
                     /locus_tag="ACHOQV_003875"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP9632169.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7407..8708
                     /gene="dcuA"
                     /locus_tag="ACHOQV_003876"
     CDS             7407..8708
                     /gene="dcuA"
                     /locus_tag="ACHOQV_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFP9632170.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8824..9162
                     /gene="cutA"
                     /locus_tag="ACHOQV_003877"
     CDS             8824..9162
                     /gene="cutA"
                     /locus_tag="ACHOQV_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFP9632171.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9138..10835
                     /gene="dsbD"
                     /locus_tag="ACHOQV_003878"
     CDS             9138..10835
                     /gene="dsbD"
                     /locus_tag="ACHOQV_003878"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFP9632172.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIANVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10872..11447
                     /locus_tag="ACHOQV_003879"
     CDS             10872..11447
                     /locus_tag="ACHOQV_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9632173.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDAWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11554..11629
                     /locus_tag="ACHOQV_003880"
     tRNA            11554..11629
                     /locus_tag="ACHOQV_003880"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11587..11589,aa:Phe,seq:gaa)
BASE COUNT         2843 a         3080 c         2930 g         3052 t
ORIGIN      
        1 tttatgttat tttaacaact attactacca agcccattta tctggcttat aaattaaaat
       61 taattattta attgagaaat ggttagggag aacctacatg gccataagca tcaagggtgt
      121 taataccggt gttattcgca agagcaataa tttcattgca ctggcgctga aaattaaaga
      181 gcctcgcaat aaagaatcgt tgttctttct gtctgtcatg gaactgcgcg accttctgat
      241 tgcgctggaa agccgtctgc atcaaaagca taaactggat gctgctgcac gccttcagta
      301 tgagcaagct cgcgataaag tcattaagaa aatggtagaa aatatcccag aaattctggt
      361 tgatgagctt aaaaatgccg atattaatcg tcgggttaac acgctggaac ttaccgataa
      421 tcagggcgaa aatcttacct tcgtattaac actacatgac ggtagcaaat gtgagttagt
      481 catcaatgaa ttacagattg aaatgctggc gcgagcaatc attcatgcaa ttaataatgc
      541 ggaaatgcgt gaactggcat tacgtattac ttcactgtta gatttcctgc cgttatacga
      601 cgttgactgc caggacaatg gcaatctgga atatgacacc tattcccaac cggaatggaa
      661 acataacttg ttcaaccatt atctggcggt gctttaccgc tttaaagatg aaagtggcaa
      721 agaacaattt agcggcgcag tggtgaaaac gcgtgaagca acgccgggta aagaagtgga
      781 ggcgatcact cgccgaatgc ttgatttcag cccccgactg aaaaaactcg cgggtgtgcc
      841 atgtcaggtc tatgttcgca ccgtagcggc aaataacgcc caaccgctaa cccaggatca
      901 atgcttacgc gcgctgcatc acttacgtgt tcaatccacc agcaaaactg caccacaggc
      961 gaaataatct catctgtttt actacgaggc tggaagtatc cagcctctgg atcacatcag
     1021 ttcgggcact tataaacctg accgataatc tggctatccg tcgggacaaa actggacaac
     1081 attccctgcg acgggctgct gatgccataa atcacgttac cgcccattgc agccgcctgg
     1141 ttgcgcagat cgtttgctgc gccgcgcata gaaccgccct cttctccgtg ttgcccggaa
     1201 agccagttgc tttgcttacc tgtcgcagta ccaatcagct ggcactctgc gcctggctgc
     1261 tcgtccacaa tgcgtacact ctgaccggca gcactcaatt cgttgctgga gctacaaccc
     1321 gccatcagta gcgcggcacc gacaatccct gctaagtatt ttacgtgcat gttattcccc
     1381 ataatcaatg agctggacgc cactcgcgtc gtccgtgtct gaatcttata ctagaaagat
     1441 gaccaaaaga aaaaccccca gacatttctg cccgggggtt tgtttatttc tgcgaggtgc
     1501 agggcaatta catcatgccg cccatgccac ccatgccgcc cataccgcca gcagcgccta
     1561 agtcagctgc atcgtttttc ggcaggtcgg taaccatgca ttcggtggtg atcatcaggc
     1621 cagccacaga agctgcgtac tgcagagcag aacgagttac tttggttgga tccaggatac
     1681 ccatgtcgat catgttgccg tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc
     1741 cgcctttaac ggtgttagca acaacagacg gttcttcgcc gcagttcaat acgatctgac
     1801 gcagcggagc ttccattgca cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct
     1861 gaccacgcag gtcagccagt ttagacgcta cgcggatcag cgcaacacca ccaccagcaa
     1921 ccacgccttc ttctaccgca gcacgggtcg cgtgcagggc atcttcaacg cgtgcttttt
     1981 tctctttcat ttcaacttcg gtagcagcac ccactttgat aactgcaacg ccgcctgcca
     2041 gtttcgctac gcgctcctgc agtttttcac ggtcgtagtc agaagttgct tcttcaatct
     2101 gctgacggat ctgagcaaca cggccctgga ttgcagcttc ttcacccacg ccatcaatga
     2161 tggtggtggt gtctttgttg atcacaacac gtttagcctg acccaggtct tccagggttg
     2221 ctttttccag ctccataccg atctcttcag agattacggt accgccagtc agggttgcga
     2281 tatcctgcag catagcttta cgacgatcgc cgaagcccgg agctttaacc gcagcaactt
     2341 tcacgatgcc acgcatggtg ttaacaacca gagttgccag cgcttcgcct tctacatctt
     2401 cagcgatgat cagcagcggt ttgcctgctt tggcaacagc ttccagaacc ggcagcattt
     2461 cgcggatgtt ggagattttc ttgtcagcca gcaggatgaa cgggctttcc agttctactg
     2521 cgccagtttc cggcttgttg ataaagtaag gagacaggta gccacggtcg aactgcatac
     2581 cttcaaccac gtccagttcg tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt
     2641 ctttaccgac tttgtccatc gcttcagcga tcagtttacc tacggtttcg tcggagttag
     2701 cggagatggt accaacctga gcaatcgctt tagagtcaga gcatggtacg gacagcgctt
     2761 tcagttcttc aactgcagcg gtaaccgctt tgtcgatacc acgtttcagg tccatcgggt
     2821 tcatgcccgc agcaacagct ttcagacctt cagtgatgat agcctgagcc agtacggttg
     2881 cagtggtggt accgtcgcct gcagcgtcgt tcgctttgga ggcaacttct ttcaccatct
     2941 gcgcacccat gttttcgaac ttgtcttcca gttcgatttc acgagcaacg gaaacaccat
     3001 ctttggtgat ggtcggtgca ccgaaagatt tatccagaac tacgttacgg cctttcggac
     3061 cgagggtaac tttcactgca tctgccagta cgtttacgcc gcgcagcatt ttcacacgag
     3121 cgtcgttacc gaattttacg tctttagctg ccattatctt tattccttaa attcgtatgt
     3181 tcagtgtcgt gcgcggatta cgcttcaaca attgccagaa tgtcgctttc ggacatgatc
     3241 aacacttctt cattgtcgat cttctcggat ttcacaccgt agccatcgtt gaaaataacg
     3301 atgtcgccaa ctttcacgtc cagcggcttc acttcgccat tttcaaggat acggccattg
     3361 ccgacagcca gcacttcgcc gcgggtggat ttagccgctg cagagccggt cagaacgatg
     3421 ccgccagcag atttagtttc aacttcttta cgcttgacga tcacgcgatc atgcaatgga
     3481 cgaatattca ttgataactc tcctttgaga aagtccgtat ctgttatggg tgacgccgga
     3541 gcttacgtgg tttcccggct ggtgaccaga gaaatgggga tgaggcttcg ccccttcaag
     3601 ggggaaaaag aaaaaaaatt ctgatcagca caaaatcggg tgaaaaccct gattcacctc
     3661 acatttcatc gcaatttctt catcgccgtc ataagcgaat ctgattgtgc taccatcgaa
     3721 aatctacgca gttgcccaaa atttgggcgc aatcggacat caacccatga gtggactcaa
     3781 acaagaactg gggctggccc agggcattgg cctgctatcg acgtcattat taggcactgg
     3841 cgtgtttgcc gttcctgcgt tagctgcact ggtagcgggc aataacagcc tgtgggcgtg
     3901 gcccgttttg attatcttag tgttcccgat tgcgattgtg tttgcgattc tgggtcgcca
     3961 ctatcccagc gcaggcggcg tcgcgcactt cgtcggtatg gcgtttggtt cgcggcttga
     4021 gcgagtcacc ggctggctgt ttttatcggt cattcccgtg ggtttgcctg ccgcactaca
     4081 aattgccgcc gggttcggcc aggcaatgtt tggctggcat agctggcaac tgttgttggc
     4141 agaactcggt acgctggcgt tggtgtggta tatcggtact cgcggtgcca gttccagtgc
     4201 aaatctacaa accgttattg ccggacttat cgtcgcgctg attgtcgcta tctggtgggc
     4261 gggcgatatc aaacctgcga atatcccctt cccggcacct ggtaatatcg aacttaccgg
     4321 gttatttgct gcgttatcag tgatgttctg gtgttttgtc ggtctggagg catttgccca
     4381 tctcgcctcg gaatttaaaa atccagagcg tgattttcct cgtgctttga tgattggtct
     4441 gctgctggca ggattagtct actggggatg tacggtagtc gtcttacact tcgacgccta
     4501 tggtgaaaaa atggcggcgg cagcatcgct tccaaaaatt gtagtgcagt tgttcggtgt
     4561 aggagcgtta tggattgcct gcgtgattgg ctatctggcc tgctttgcca gtctcaacat
     4621 ttatatacag agcttcgccc gcctggtctg gtcgcaggcg caacataatc ctgaccacta
     4681 cctgtcacgc ctctcttctc gccatatccc gaataatgcc ctcaatgcgg tgctcggctg
     4741 ctgcgtggtg agcactttgg tgattcatgc tttagagatc aatctggacg ctcttattat
     4801 ttatgccaat ggcatcttta ttatgattta tctgttatgc atgctggcag gctgtaaatt
     4861 attgcaagga cgttatcgac tactggcggt ggttggcggg ctgttatgcg ttctgttact
     4921 gacaatggtc ggctggaaaa gtctctatgc gctgatcatg ctggcggggt tatggctgtt
     4981 tctgccaaaa cgaaaaacgc cggaaaatgg cataaccaca taatccggcg tttcgacatt
     5041 aatcctggcg atcgtcttta tgatcaaggc ggtcgcggtc atcatccttt cgctggtact
     5101 caccatcaaa agtattaccg ccaccggtcc cggcgctaaa accgccgcct ggcatgcgag
     5161 aaaagcgcaa atgcggcatc aacttcactg tcagatgctt ttgcaccggc ggcaacaaaa
     5221 gcagaagacc gaggaagtcg gtgaaaaagc ccggtaataa aagcagcaaa ccggcaatga
     5281 tcagcgaaac acttttaatc atctccgccg ctgggttttc acccgccgcc attttttgct
     5341 gcatcagcac gaaattctta aagccctggt tacgtaccag tgacataccg ataactgacg
     5401 tgaatataac cagcacgaga gtcagcaata cccccaatac atgggcaacc tgaataaaga
     5461 ttgaaatctc aatatagaca taaaggaaaa tggcaataaa aggtaaccag cgcaaaggtt
     5521 tctcctgtaa tagcagccgg ttaaacccgg ctacctgaat gggttgcgaa tcgcgttcag
     5581 cttatattgt ggtcattagc aaaatttcaa gatgtttgcg caactatttt tggtagtaat
     5641 cccaaagcgg tgatctattt cacaaattaa taattaaggg gtaaaaaccg acacttaaag
     5701 tgatccagat tacggtagaa atcctcaagc agcatatgat ctcgggtatt cggtcgatgc
     5761 aggggataat cgtcggtcga aaaacattcg aaaccacata tattctgtgt gtttaaagca
     5821 aatcattggc agcttgaaaa agaaggttca catgtcaaac aacattcgta tcgaagaaga
     5881 tctgttgggt accagggaag ttccagctga tgcctactat ggtgttcaca ctctgagagc
     5941 gattgaaaac ttctatatca gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg
     6001 tatggtaatg gttaaaaaag ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa
     6061 aagtgtagcg aatgccatca ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat
     6121 ggatcagttc ccggtagacg tctaccaggg cggcgcaggt acttccgtaa acatgaacac
     6181 caacgaagtg ctggccaata tcggtctgga actgatgggt caccagaaag gtgaatatca
     6241 gtacctgaac ccgaacgacc atgttaacaa atgtcagtcc actaacgacg cctacccgac
     6301 cggtttccgt atcgcagttt actcttccct gattaagctg gtagatgcga ttaaccaact
     6361 gcgtgaaggc tttgaacgta aagctgtcga attccaggac atcctgaaaa tgggtcgtac
     6421 ccagctgcag gacgcagtac cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct
     6481 gctgaaagaa gaagtgaaaa acatccaacg taccgctgaa ctgctgctgg aagttaacct
     6541 tggcgcaaca gcaatcggta ctggtctgaa cacgccgaaa gagtactctc cgctggcagt
     6601 gaaaaaactg gctgaagtca ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc
     6661 gacctctgac tgcggcgctt atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa
     6721 gatgtccaaa atctgtaacg acctgcgctt gctctcttca ggcccacgtg ccggcctgaa
     6781 cgagatcaac ctgccggaac tgcaggcggg ctcttccatc atgccagcta aagtaaaccc
     6841 ggttgttccg gaagtggtta accaggtatg cttcaaagtc atcggtaacg acaccactgt
     6901 taccatggca gcagaagcag gtcagctgca gttgaacgtt atggagccgg tcattggcca
     6961 ggccatgttc gaatccgttc acattctgac caacgcttgc tacaacctgc tggaaaaatg
     7021 cattaacggc atcactgcta acaaagaagt gtgcgaaggt tacgtttaca actctatcgg
     7081 tatcgttact tacctgaacc cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat
     7141 ctgtgccgaa accggtaaga gtgtacgtga agtcgttctg gaacgcggtc tgttgactga
     7201 agcggaactt gacgatattt tctccgtaca gaatctgatg cacccggctt acaaagcaaa
     7261 acgctatact gatgaaagcg aacagtaatc gtacagggta gtacaaataa aaaaggcacg
     7321 tcagatgacg tgcctttttt cttgtgagca gtaacttaaa aataacaatc taatatcaac
     7381 ttgttaaaaa aacaaggaag gctaatatgc tagttgtaga actcatcata gttttgctgg
     7441 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7501 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7561 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7621 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7681 cgatcgtgac ctatttcctg acaatctttg ctggtactgg caacatctct ctggcgacac
     7741 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctgtcta
     7801 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     7861 acatgtcttc cgtaatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     7921 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     7981 ccaaactctc tgacgatccg atttatcgca agcgtctgga agaaggcctg gttgaactgc
     8041 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8101 tgggcgtagt tggcgttgtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8161 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8221 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8281 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8341 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8401 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8461 aagcactgat gccgatggct ctggcactga acgtttctcc gctgaccgct gttgcttctt
     8521 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8581 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8641 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8701 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8761 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     8821 tttatgcttg atgaaaaaag ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg
     8881 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     8941 gcgaccttga tccccggcgc tacttctctc tattactggg aaggtaagct ggagcaagaa
     9001 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9061 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9121 gacacagatt acctctcatg gctcaacgca tctttacgct gatcctgcta ctttgcagca
     9181 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9241 aagcctttgc ttttgatttt cagcaaaacc aacatgacct gaatctgacc tggcagatca
     9301 aagacggtta ttacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9361 ccaacgtgca gctgccacaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9421 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9481 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9541 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgttccgc
     9601 agcaagagca gcccaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9661 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggtatcgtgc
     9721 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9781 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
     9841 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
     9901 tggcgatgtc aatgtttggt ttgtttaccc tgcaactccc ctcttcgctg caaacgcgcc
     9961 tcacgctgat gagcaatcgc caacagggcg gctcacctgg cggtgtgttt gttatgggag
    10021 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgttgt
    10081 atatcgccca aagcgggaac atgtggctgg ggggcggcac gctttatctc tatgcgttgg
    10141 gcatgggcct gccgctgatg ctaataaccg tctttggtaa ccgcctgctg ccgaaaagcg
    10201 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10261 tcctgctgga gcgagtgatt ggcgatatat ggggattacg cttgtggtcg gcgcttggtg
    10321 tcgcattctt tggctgggcc tttatcacca gcctacaggc caaacgcggc tggatgcgcg
    10381 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10441 catttggtgc aacacatacc gcgcaaactc agacgcatct caactttaca caaatcaaaa
    10501 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccagtg atgttagatc
    10561 tctatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10621 aggtgcaaaa agcgttagca gacacggtct tacttcaggc taacgtaact gccaacgacg
    10681 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10741 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10801 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    10861 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    10921 aagggattgc caacaccacg cttgagatgg ttgctgaacg tgtggattac ccactggacg
    10981 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgttg cgttatctta
    11041 gccaacagat agatgcctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11101 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11161 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11221 tggccgatca gcaaaaaagc gcggcctacg atttcaccca cgaactgtta accacactgg
    11281 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11341 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac cgcacatcgg ctggcggaag
    11401 atatcctgcg cttcgcccgc tgccgccagg gtggtgcttt gacctgacag aaacacagaa
    11461 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11521 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11581 gggattgaaa atccccgtgt cggtggttcg attccgcctc cgggcaccat ataccagaac
    11641 acgctggtta agggatgaga tccttgacgg gcggttaata caggatgcgg catcaaagga
    11701 tttccgtttg tgtaagttgt ggttacactg attgagttcg gtatcgtatc ctcaaacagg
    11761 gtgtcttttg cggagacgcc ctgttctcgt taacactgta tgcttacatc gctgattcag
    11821 cctgtcaaga tttattcccc cctttgcaaa tcttgctcac tgtgcgctca tctgtataca
    11881 aactcagggc atcttccagt gtctg
//
LOCUS       DAZKGF010000147        11896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZKGF010000147 DAZKGF010000000
VERSION     DAZKGF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.147"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(62..343)
                     /gene="yehE"
                     /locus_tag="ACHOQV_003881"
     CDS             complement(62..343)
                     /gene="yehE"
                     /locus_tag="ACHOQV_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFP9632174.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(606..1715)
                     /gene="apbC"
                     /locus_tag="ACHOQV_003882"
     CDS             complement(606..1715)
                     /gene="apbC"
                     /locus_tag="ACHOQV_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFP9632175.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPNMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1847..3880
                     /gene="metG"
                     /locus_tag="ACHOQV_003883"
     CDS             1847..3880
                     /gene="metG"
                     /locus_tag="ACHOQV_003883"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP9632176.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEEIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4022..7810
                     /locus_tag="ACHOQV_003884"
     CDS             4022..7810
                     /locus_tag="ACHOQV_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFP9632177.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     AAAAEKAGLKLIAEKVKKGYVEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEISQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVTALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISHPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLNLESICTITDTEAKEFQTHWDWEPHKPSEGAKNFLY
                     SLGYRRWDFDTYKYIGASDSAIDAWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPM
                     QKAIKLWEFLSKEPHTAIKPVMLYLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAP
                     AVARAFNKLKTLRQDASSWLLKYPEHAITGLLPAALGKASEAQDNARAALRMLTENGH
                     QPLLQEIARRYNQPEVTDAVNALLALDALDNHPTKIPTLPTFYQPSLWTRPLLKANAQ
                     SLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKE
                     SWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIA
                     QKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVS
                     FDETLKPFVRDASGSRLKDLPKPNKSDDELQANNAVNRYKLLKKDARTVAAQQVARLE
                     SAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTAD
                     DDLFTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERN
                     ASELTRWAGRKCPSGRVMGLANKGWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAV
                     GMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE
                     "
     gene            7820..11452
                     /gene="yehI"
                     /locus_tag="ACHOQV_003885"
     CDS             7820..11452
                     /gene="yehI"
                     /locus_tag="ACHOQV_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFP9632178.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQHEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDQKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPWLSTQARAVLNSYLSAPPKTVIDSTDNSQMPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLASTESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYPGRVEEIMNGWSSPEAQLAEQALRSGHVEVLINIWEND
                     SYSRYRPEKSVWNLYLLAQLPREMALPFWLRINEKKHLSAGEDYFLIIFGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTAANRWQRTDVWPALEHLLKQSPIEIYPA
                     RMPKAPDFWHPLMWSRPRLIINHQLITDDALEIIGEMLRFTQGGRFHSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSTR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRTDDDQLKAPE
                     ALARLKGLKKDTTQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTEGMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYGLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11514..11867
                     /locus_tag="ACHOQV_003886"
     CDS             11514..11867
                     /locus_tag="ACHOQV_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632179.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRKLQNIFNQ
                     LIEYYFIKGGSGFPP"
BASE COUNT         3100 a         3037 c         2901 g         2858 t
ORIGIN      
        1 cttaaatcat tcatgtgaat gatttaagct ttctaaatcc gattaaaacc agggtggcaa
       61 attaatcgta gcgatttaac acgattcgac gtttgctatc gctatctgca gcaatcactt
      121 ccgtcgttac acctttaact ggcgaagaaa cctgatgacg ggtatcaaca ttatacgtta
      181 actgaccaca atgctgttga ggttgaccat taaccatagc catactgcat gtcgggggac
      241 tgatcctacc attaatcgtt aatgtggcag tttctgaaga gaacgcggga gaggccaatc
      301 cataggcaag aaaaataatt cctgacagcc aatacttatt cattgaacgt tatccctgta
      361 gtaaaggtta tgcctggata gaatgagtgc ataacaaact atagctgtac atccactaca
      421 cagccacgaa ggatgataat gaagcattgc ctgtacgatc aatcgacttt gtagagtttc
      481 ggacgaaggt ccgcagaata ttcgcagcat taaataagtg ttcattaatg cggcaaaaac
      541 aaatacaccg catcaatgta acatctctgc ggtgtatgaa gaataaccag aatggttaat
      601 acgcgttaga ccgcgcggaa ggaaatctcg cctggaatga cttcaccctg ccagtaaagc
      661 tgagctgcaa cgcggtcagc cagctggcgg tagatagcgg taaattcgct ctcaggacgg
      721 ctgataaccg ttggcgtgcc tttatcgaga tcctcacgta aggagatgtg taacggcatc
      781 tgccccagca gctgtgtgtg gtatttctcg gccagtttct ctgcaccacc ggtaccgaaa
      841 atcggttcat ggtgaccaca gttactgcaa atatgcacac tcatgttctc gacaataccg
      901 agcaccggaa cctcgacttt ctcgaacatc acaatgcctt ttttcgcatc gatcaacgcg
      961 atgtcctgcg gggtggtgac cacgactgca ccggttactg gaatgttttg tgccaatgtc
     1021 aattgaatgt caccggtacc tggcggcata tcaagcacca gatagtcgag atccggccac
     1081 aaggtttcct gcaacatttg cattaacgcc ttgctcgcca ttgggccacg ccacaccatc
     1141 gcattgtcgt ctgtcaccag ataaccaatt gaattagttg ccaggccatg agacataatc
     1201 ggtgccatat gggtaccgtc cggcgaagtt ggacgctgat tttctgcacc cagcatgtta
     1261 gggattgatg ggccgtagat atcggcgtcc agaatgccga ctttcgcccc ttccgccgcc
     1321 agtgccaacg ccaggtttac cgccgtggag gatttaccca cgccaccttt accggaactg
     1381 accgcgataa tgttcttcac gccgttaatt cccggctgat tcttcacgcg tttcagagtg
     1441 gctatgttat gcgacagttt ccagtcgata gctttcgctc cggtgatgcg cagcagttcg
     1501 gcgctacatt gctcttttaa ctcttcgaac gcgctatgcc agacgaatgg cataaccagt
     1561 tccacatgca gcgtgtcgtc catccaggcg acatggtgca acgctttcag cgttgtgagg
     1621 ttatgcttca gggttgggtg ctgaaaattg gccagtgtcc cggcgaccat tgcgcgcaag
     1681 gcttctggtg atttggcctg ggattgttcg ttcatcccga ctcctttttt gtatagataa
     1741 accatcagtt gatagtttac ctgaagaata tagagaagta cttacttaac attttcccat
     1801 ttggtactat ctaagccctt ttcactatta agaagtaatg cctactatga ctcaagtcgc
     1861 gaagaaaatt ctggtgacgt gcgcactgcc gtacgctaac ggctcaatcc acctcggcca
     1921 tatgctggag cacatccagg ctgatgtctg ggtccgttac cagcgaatgc gcggccacga
     1981 ggtcaacttc atctgcgccg acgatgccca cggtacacca atcatgttga aagctcagca
     2041 gcttggtatc actccggagc agatgattgg cgaaatgagt caggagcatc agactgattt
     2101 cgcaggcttt aacatcagct atgacaacta tcactcgacg cacagcgaag agaaccgcca
     2161 gttgtcagaa cttatctact ctcgcctgaa agaaaacggt tttattaaaa accgcaccat
     2221 ctctcagctg tacgatccgg aaaaaggcat gttcctgccg gaccgttttg taaaaggcac
     2281 ctgcccgaaa tgtaaatccc cggatcaata cggcgataac tgcgaagtct gcggcgcgac
     2341 ctacagcccg actgaactga tcgagccgaa atcggtggtt tctggcgcta ccccggtaat
     2401 gcgtgattct gaacacttct tctttgatct gccctctttc agcgaaatgc tgcaggcatg
     2461 gacccgcagc ggtgcgttgc aggagcaggt ggcaaataaa atgcaggagt ggtttgaatc
     2521 tggcctgcaa cagtgggata tctcccgcga cgccccttat ttcggttttg aaattccgaa
     2581 cgcgccgggc aaatatttct acgtctggct ggacgcaccg attggctaca tgggttcttt
     2641 caagaatctg tgtgacaagc gcggcgacag cgtaagcttc gatgaatact ggaagaaaga
     2701 ctccactgcc gagctgtacc acttcatcgg taaagatatt gtttacttcc acagcctgtt
     2761 ctggcctgcc atgttggaag gtagcaactt ccgcaagccg accaacctgt ttgttcacgg
     2821 ctatgtaacg gtgaacggcg cgaagatgtc caagtctcgc ggcaccttta ttaaagccag
     2881 cacctggctg aatcattttg acgctgacag cctgcgttac tactacactg cgaaactctc
     2941 ttcgcgcatt gatgatatcg atctcaacct cgaggatttc gttcagcgtg tgaatgctga
     3001 tatcgtcaac aaagtggtta atctggcgtc ccgtaacgcg ggctttatca acaaacgttt
     3061 tgacggcgtg ctggcaagcg aactggctga cccgcaactg tacaaaacct tcactgatgc
     3121 cgctgaagag attggcgaag cgtgggaaag ccgtgaattt ggtaaagcag tgcgcgaaat
     3181 catggcactg gctgacctgg ctaaccgcta tgtcgatgaa caggctccgt gggtggtggc
     3241 gaagcaggaa ggtcgcgatg ctgacctgca ggcgatttgc tctatgggca ttaacctgtt
     3301 ccgcgtactg atgacttacc tgaagccggt actgccgaaa ctgactgaac gagcagaagc
     3361 attcctgaat acggaactga cctgggatgg tatccagcaa ccgctgctgg gccataaagt
     3421 gaatccgttc aaagcactgt ataaccgtat cgacatgaag caggttgaag cactggtgga
     3481 agcgtctaaa gaagaagtga aagccactac cgcgccagtt actggcccac tggcagatga
     3541 tccgattcag gaaaccatca cctttgacga cttcgctaaa gttgacctgc gcgtggcgct
     3601 gattgaaaac gcagagtttg ttgaaggttc tgacaaactg ctgcgcctga cgctggatct
     3661 cggcggtgaa aaacgcaatg tcttctccgg cattcgttct gcttacccgg atccgcaggc
     3721 acttattggt cgtcacacca ttatggtggc taacctggca ccacgtaaaa tgcgcttcgg
     3781 tatctccgaa ggcatggtga tggctgctgg tcctggcggg aaagatatct tcctgctaag
     3841 cccggatgcc ggtgctaaac cgggtcatca ggtgaaataa tccccccttc aaggcgctgc
     3901 atcgacagcg ccttttcttt ataaattcct aaatttgttt tcttgcgatt ttgtctctct
     3961 ctaacccgca taaatactgg tagcatctgc attcaactgg ataaaattac agggatgcag
     4021 aatgagacac tttatctatc aggacgaaaa atcacataaa ttctgggcgg tggagcagca
     4081 gggaaacgaa ctgcacatca actggggtaa agtcggcacc aacggacaaa gccaggtaaa
     4141 aagttttgcg gatgcagcag cggcggaaaa agcgggactg aagctgattg cagagaaggt
     4201 gaagaagggg tatgtggagc aagcgaagga taattctttg caaccttccc aaacggtaac
     4261 ggactctctc aaggtagctg acttatccac cattattcag gaacaaccct ctttcgtagc
     4321 agaaacccgt gcggctgaca aaaatacaga tgctgtttta ccgtggctgg cgaaagatat
     4381 tgctgtcgtt tttccgcccg aagttgtaca caccacgtta agtcatcgcc gctttcccgg
     4441 agttcctgtt cagcaagcag acaaattgac ccaattacgt cgcttagcct gtagtgtgtc
     4501 gcaacgggat aataaaacag ccacatttga cttcagcgcc tgttctttag aatggcaaaa
     4561 caccgtcgcc caggcgatca gtcagatcga cggcctgaaa acaacacagc taccatcacc
     4621 cgtaatggct gtactcactg cacttgaaat gaaatgcaca agatataaag agcgtgagga
     4681 tgttatggat cagattattc aggaaggcgg tctggaatat gctactgatg taataataca
     4741 tcttcaacaa atatctattg aatgggatta tgtcaacaat aatattgttt tcctgtcgtc
     4801 tggcatttca cctgattact tgcagcaata ttccagcttt gaattacgcc tacgtaaaca
     4861 tttatcactg gcggaagagt ctctctggca aaaatgtgca caaaaactta ttgccgcaat
     4921 tccacatatt ccagaatggc ggcaaccatt aattgctttg ttattacccg aaaaaccaga
     4981 aattgcacat gaaatttccc agcgtctact ggggcaaaag aaattaccct cgcttgagtg
     5041 gctaaaaata gtggcgactg atgagcacat tcttgcctca ttagaaaaat atcacgagcc
     5101 atatgccatt tttgatgatt actattgtgg tgcgatatgg tcagccaccg tattacagga
     5161 gcaaggtgtt acagccctgc cccgatttgc tccctatgcc gcaagtgact actgcgccga
     5221 tgtgttgcgt catatcaatc atccgttcgc attaacactg cttatacgtg tagccgggca
     5281 tactaaacgc tgtcacgatc gaatgacgaa agcctgcgct gcgttcccac acgcagcact
     5341 ggcagcactg gcggaacttc tagtgcaaaa agaagagaat agttggcgca ttatgctaat
     5401 gacgatgctt atctcgcatc cgacactagc agaacaggtc attccctggc tttcgacacc
     5461 cgcagttgca gtgctgaaat catgtcagca acaactgaca cagccctcaa accatgccag
     5521 cgccgatcta ctgccggcca tagtagtctc cccaccatgg ctttcgaaaa agaaaaaatc
     5581 gccgattccg gtgctggatt tagcaccgct caacctcgaa tccatatgca caataaccga
     5641 cacagaagct aaagagtttc aaactcattg ggactgggaa ccgcataagc caagtgaagg
     5701 tgctaaaaat ttcctttact cgctggggta tcgccgctgg gattttgaca catataaata
     5761 tatcggcgct tctgactcgg caattgacgc atgggaacgt gaggacttcg ctacactcat
     5821 ccaaatgttt aaagcccacc atgcaccgta tcagggggag tggcatctca actcacttcc
     5881 ctttttacct atgcaaaaag cgattaagct atgggaattt ctcagcaaag agccgcatac
     5941 cgcgataaaa ccagtcatgc tttatttgcg actggcaggt atgagtggtt ttctacactc
     6001 gttttcacgc taccctcaag aaggttttgc ggttgctaat tattttgccg caacggaact
     6061 ggctcctgcc gtcgcccgcg ccttcaacaa actcaaaaca ttacgacaag acgccagtag
     6121 ctggctgctg aaatacccgg aacacgccat taccggcctg ctgcctgcag cgctcggcaa
     6181 agccagtgaa gcacaggaca acgcccgtgc tgccttgcgt atgcttaccg aaaacggtca
     6241 tcagccattg ctgcaagaaa tcgcccggcg ttataaccag ccggaagtaa ccgatgcggt
     6301 gaacgctcta cttgcgctcg atgccttaga taatcacccg acaaaaatcc ccactcttcc
     6361 gaccttttat cagccatcgc tctggacgcg cccgttatta aaagcaaacg cccaatcact
     6421 gcctgatagc gcactcctcc acctcggtga aatgctccgc ttccctcagg aagaggcact
     6481 gtatccggga ttattgcagg tgaaagatgc ctgtaccacc gactcactgg ctgaatttgc
     6541 ctgggatctg tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt
     6601 cactgcgtta ggcgttctcg gtaacgatga caccgcccgc aaactgacgc ctttgattcg
     6661 cgcctggcct ggtgaatccc agcataaacg cgccaccgtc gggttggata ttctcgctgc
     6721 tatcggtagt gatatcgccc tgatgcagct taacggcatc gcccagaaac tgaaattcaa
     6781 agcattacag gagcgggcaa aagaaaaaat tgccgacatt gccgagagcc gcgaactcac
     6841 ggtggcggag cttgaagatc ggttagcacc ggatctcggt ctggatgata atggttcgtt
     6901 gttgctggat ttcggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt
     6961 tgtgcgtgac gcttccggca gtcgtctgaa agacctgccc aaaccaaaca aaagcgatga
     7021 tgaattacag gcgaacaatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac
     7081 cgtcgccgcc cagcaggtag caaggctgga atccgccatg tgcctgcgtc gccgctggtc
     7141 accggaaaac tttcagctct tcctggttga gcatccgctg gtccgccact taacccgccg
     7201 tctgatttgg ggcgtttata gcgccgaaaa ccagctactg gcttgctttc gcgtagcgga
     7261 agataacagc tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc
     7321 tattggtatt cctcacgttc tggaaatatc accgacggat gctgccgcct tcgggcagct
     7381 ttttgccgac tacgaactgt taccaccgtt ccgccagctt gatcgtaaca gttacgcgct
     7441 gacagaagcc gagcgcaatg ccagcgaact gacacgctgg gcaggcagaa aatgcccgag
     7501 tggtcgggta atggggctgg caaataaagg ctggatgcgt ggtgaaccgc aggacggtgg
     7561 ctggatcggc tggatgatca aacctctggg ccgctggtcg ttaatcatgg aaatcgatga
     7621 aggttttgcg gtcggcatgt cgccagccga actcagtgcc gagcaactct taagcaagct
     7681 gtggctatgg gaaggcaaag cggaaagcta tggctggggg agtaattcaa cacaggaagc
     7741 gcagttctcc gtactcgatg ccatcaccgc cagcgagcta attaacgata ttgaagccct
     7801 gtttgaataa ggaaagagca tggacaagga attaccgtgg ctggcggata acgcccaact
     7861 ggaactgaaa tataaaaaag gcaaaacacc actcagccat cgcaattggc caggcgaacc
     7921 ggtacctgtt atcactgaaa acatcattca gacactgggt gatgaattgc tacaaaaagc
     7981 agagaagaaa aaaaacattg tctggcgtta tgaaaacttt tcactggagt ggcagtccgc
     8041 catcacgcag gccatcaact tgatcggcga acacaaaccc tcaatcacgg cccggacaat
     8101 ggcggcgcta gcctgtatcg cgcaaaatga tagccaacag ttgctcgacg aaatcgtcca
     8161 acacgagggg ctggaatatg caactgaggt ggtgattgca cgccagttta ttgtacggtg
     8221 ttatgagagt gatcctctgg tagtaacatt gcaatatcag aacgaggact atggctatgg
     8281 ttatcgctca gaaacctata acgaattcga tctccgattg cgtaagcatc tctctctggc
     8341 agaggaaagc tgctggcagc gttgcgccga caaactcatt gccgcactgc caggaataac
     8401 caaagttcgc cgccctttta ttgcgctgaa cctcccggaa aaacccgaaa tcgccaacga
     8461 gctggtaagc cttgaatgtt cgcaaactca ttttcgctca aaagagtggt taaaagttgt
     8521 tgctgatgac cctaaggcgg tgaaagaact cgcacgttac tggagccagg atatatttag
     8581 cgatcgagaa gccagctaca tgtcgcatga aaaccacttc ggctacgcgg cctgcgctgc
     8641 ccttttgcgc gaacaaggac tggcagccgt tccgcgcctc gcgatgtatg cacataaaga
     8701 agattgcggc agtctgctgg tacaaattaa ccatccgcaa gtcatccgca ccttgctact
     8761 ggtggctgat aaaaacaaac ccagcctgca acgtgtggct aaatacagta aaaacttccc
     8821 ccatgcgacg ctcgccgcac tggcagaact gctggcgtta aaagcccccc ctgcgcgccc
     8881 tggttatccc atcatcgaag accaaaagct gcctgcacag caaaaagcac gtgatgaata
     8941 ctggcatacg ctgttacaaa cgctgatggc atcgcagcca caactggcag aagaggtgat
     9001 gccgtggtta agtactcaag cccgggcagt gctgaatagt tatttatcgg caccgcccaa
     9061 aacggttatt gatagcaccg ataacagcca aatgcctgaa atcctcgttt caccaccgtg
     9121 gcgtagtaag aaaaaaatga cagctccacg tcttgatttg gcaccgctcg aattaacccc
     9181 gcaagtttac tggcaaccag gcgaacaaga gaggcttgcc tccactgagt ctgcccgtta
     9241 tttcagcacg gaatctcttg ctgaacgcat ggaacaaaaa agtggacgag ttgtattaca
     9301 ggaactgggt tttggggatg atgtatggct gtttctgaat tatatactcc ccggaaaact
     9361 ggatgctgca cgcaattcac tcattgttca gtggcattac tacccagggc gggttgaaga
     9421 gatcatgaat ggctggagct ccccggaagc tcaattagca gaacaggcgc ttcgcagcgg
     9481 tcacgtagaa gtgttaatta acatatggga aaatgacagc tactcacgtt atcgtccgga
     9541 aaagagtgtc tggaacctgt atttattggc acagttgccg cgtgagatgg ctttgccttt
     9601 ctggctacgt atcaatgaga aaaagcatct gtctgcgggt gaggactatt ttctcattat
     9661 cttcggattg gatgctctac caggtctgct gttggctttt tcacatcgtc caaaagaaac
     9721 atttccgtta attttaaatt ttggcgcaac agaactggcc ctgcccgttg cccgcgtctg
     9781 gcgacgtttc gcggcgcagc gtgatctggc ccgccagtgg attttacaat ggccggaaca
     9841 tacggctact gcacttatcc ctcttgtctt caccaaaccc agcgataata gcgaagctgc
     9901 attacttgcc ctacgtttac tttacgaaca gggacatggt gaattgctgc aaaccgcggc
     9961 aaaccgctgg cagcgtacag atgtatggcc tgccctggag catttactta aacagagtcc
    10021 aatagagatt tacccggcac gcatgccaaa agcccctgat ttctggcatc cgctaatgtg
    10081 gtccaggccg cgcctaatca ttaatcatca acttattacc gatgacgctc tggaaattat
    10141 cggcgaaatg ctgcgcttta cccagggggg acgttttcat agcgggctgg aacaactgaa
    10201 aacgttctgc cagccgcaaa cgctggcagc ttttgcctgg gatctcttca ctgcgtggca
    10261 acaagctggt gcccccgcaa aagacaactg ggcatttctg gcgttaagtc tctttggtga
    10321 cgaaagcacg gcacgggatc taacgacaca gatcctcgcc tggccacaag aaggcaaatc
    10381 tacccgtgct gtcagtggcc tgaacatcct taccctgatg aataatgata tggcgctgat
    10441 acagctgcat catatatcgc aacgggcgaa atcccgcccc ttacgtgata acgcggcgga
    10501 atttcttcag gtggtcgcag aaaatcgcgg gctaagccag gaagagttag cggacagatt
    10561 agtcccaaca ctgggccttg atgatccgca ggcgttgagt tttgattttg gtccccggca
    10621 gtttaccgtt cgcttcgatg aaaaccttaa cccggttatc tttgatcagc aaaacgttcg
    10681 ccagaaaagc gttccccggt tgcgcaccga tgacgatcaa ctgaaagcgc ccgaggcact
    10741 ggcccgatta aaagggctaa aaaaagatac gactcaggtg agcaaaaacc tgctcccccg
    10801 tcttgaagct gccctgcgta ccacccgacg ctggtcgctg gcagattttc attctctgtt
    10861 tgttaatcat ccctttaccc gtctggttac ccagcgatta atatggggcg tgtatccggc
    10921 aaatgaaccg cgtcgtttac tcaacgcctt tcgtgtggcc gcagaggggg agttctgcaa
    10981 tgcgcaagat gagcccattg acctgcctgc ggatgctctg attggcattg cccacccgtt
    11041 agaaatgaca gaaggaatgc gcagtgaatt tgcacagctt tttgccgatt acgaaattat
    11101 gccgcctttt cgccagttgg cgcggcgcac ggtgctgctc acacctgacg agtcaaccag
    11161 taacagcctg actcgctggg aaggtaaatc cgctaccgtt gggcagctta tgggaatgcg
    11221 atacaaaggc tgggagtcag gctatgagga cgcatttgtc tatggcctgg gcgagtaccg
    11281 gctggtcctt aagttttcac ccggttttaa ccactacaat gttgatagca aagcgttaat
    11341 gagcttccgt tctcttcgag tgtaccgtga caataaatcc gtcacttttg ccgaacttga
    11401 tgtgtttgat ttgagtgagg cgttaagcgc acctgacgtc attttccatt aaaaacacag
    11461 cgggcagtga tgcaactgcc cgttatcaac acgacttacc agcggggaaa gcgatgatcg
    11521 tgcaaaaaga gctggttgct atttacgatt atgaggtccc tgtacctgaa gatccgtttt
    11581 ccttcagact tgagatccat aaatgctctg aattatttac aggttccgtc tatcgactgg
    11641 agcgattccg gctacgtcca acatttcatc aacgtgatcg agaagatgct gacccgctaa
    11701 taaatgatgc gttgatttat ataagagatg agtgtattga tgagcggaaa ttacgaggtg
    11761 aatcacctga aactgtaata gcaattttta atcgtaaact acagaatata ttcaaccaac
    11821 taatagaata ttactttatt aaagggggaa gcggttttcc cccttagttg cgtttcaatt
    11881 tgcccattac ttcaac
//
LOCUS       DAZKGF010000148        11863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZKGF010000148 DAZKGF010000000
VERSION     DAZKGF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.148"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            69..401
                     /gene="hdeA"
                     /locus_tag="ACHOQV_003887"
     CDS             69..401
                     /gene="hdeA"
                     /locus_tag="ACHOQV_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFP9632180.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            517..843
                     /gene="hdeB"
                     /locus_tag="ACHOQV_003888"
     CDS             517..843
                     /gene="hdeB"
                     /locus_tag="ACHOQV_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFP9632181.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            907..1554
                     /locus_tag="ACHOQV_003889"
     CDS             907..1554
                     /locus_tag="ACHOQV_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP9632182.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1606..2376)
                     /locus_tag="ACHOQV_003890"
     CDS             complement(1606..2376)
                     /locus_tag="ACHOQV_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFP9632183.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRIVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(2373..3365)
                     /gene="shuU"
                     /locus_tag="ACHOQV_003891"
                     /gene_synonym="chuU"
     CDS             complement(2373..3365)
                     /gene="shuU"
                     /locus_tag="ACHOQV_003891"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFP9632184.1"
                     /translation="MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(3414..4037)
                     /gene="chuY"
                     /locus_tag="ACHOQV_003892"
     CDS             complement(3414..4037)
                     /gene="chuY"
                     /locus_tag="ACHOQV_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFP9632185.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGITRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFINRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(4037..4531)
                     /gene="hutX"
                     /locus_tag="ACHOQV_003893"
     CDS             complement(4037..4531)
                     /gene="hutX"
                     /locus_tag="ACHOQV_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFP9632186.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(4544..5881)
                     /gene="hutW"
                     /locus_tag="ACHOQV_003894"
     CDS             complement(4544..5881)
                     /gene="hutW"
                     /locus_tag="ACHOQV_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFP9632187.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLTQT
                     WQEMLNQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNDDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            complement(5901..6815)
                     /gene="shuT"
                     /locus_tag="ACHOQV_003895"
     CDS             complement(5901..6815)
                     /gene="shuT"
                     /locus_tag="ACHOQV_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFP9632188.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTATERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            7499..9481
                     /gene="chuA"
                     /locus_tag="ACHOQV_003896"
                     /gene_synonym="shuA"
     CDS             7499..9481
                     /gene="chuA"
                     /locus_tag="ACHOQV_003896"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFP9632189.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            9530..10558
                     /gene="chuS"
                     /locus_tag="ACHOQV_003897"
     CDS             9530..10558
                     /gene="chuS"
                     /locus_tag="ACHOQV_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFP9632190.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTTRGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFIADEN
                     MPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEVVTA"
     gene            complement(10619..11149)
                     /locus_tag="ACHOQV_003898"
     CDS             complement(10619..11149)
                     /locus_tag="ACHOQV_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9632191.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQHAFVI
                     IDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11302..11862)
                     /gene="slp"
                     /locus_tag="ACHOQV_003899"
     CDS             complement(11302..11862)
                     /gene="slp"
                     /locus_tag="ACHOQV_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFP9632192.1"
                     /translation="MTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQPG
                     LYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFLDP
                     VNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYGYG
                     AFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3072 a         2791 c         2965 g         3035 t
ORIGIN      
        1 caggttataa cctcagtgtc gaaattgatt cgtgacggct ctttcacttt atagttgagg
       61 atattacgat gaaaaaagta ttaggcgtta ttcttggtgg tctgcttctt ctgccagtag
      121 tgagcaatgc agcggatgca caaaaagcag ctgataacaa aaaaccggtc aactcctgga
      181 cctgtgaaga tttcctggct gtggacgaat ccttccagcc aactgcagtt ggttttgctg
      241 aagcactgaa caacaaagat aaaccagaag atgcggtttt agatgttcag ggtattgcaa
      301 ccgtaacccc agctatcgtt caggcttgta ctcaggataa aaaagccagc tttaaagata
      361 aagttaaagg cgaatgggac aaaattaaga aagatatgta attccgggaa tgcgttacat
      421 cgtatttcct tgcatattga acaggccgga atatcttctt taaaagcagc tattcctcct
      481 gtttgtatat aatctctata ttgaatgggt tacaaaatga acatttcatc tctccgtaaa
      541 gcgtttattt ttatgggcgc tgtagcggct ttgtcactgg tgaacgcaca atctgcgctg
      601 gcagccaatg aatccgctaa agatatgacc tgccaggaat ttattgatct gaatccaaaa
      661 gcaatgacac cagttgcgtg gtggatgctg catgaagaaa cagtatataa aggtggcgat
      721 accgttactt taaatgaaac cgatctcact caaattccta aagtgatcga atactgcaag
      781 aaaaacccgc agaaaaattt gtataccttc aaaaatcaag catctaatga cttgccgaat
      841 taatgaggta caagtaaaaa ggagtagcaa gttgagccat cttgctgctc ctttttgcat
      901 ttttatatga cagcagaatt tattatacga cttatacttg cggtaattgc ctgtggcgct
      961 attggcatgg aaaggcaaat gcgcggcaaa ggagcagggt tacgcacaca tgtattaatt
     1021 ggcatgggaa gcgccctgtt tatgattgtt tcgaaatatg gttttgctga cgtgctgtct
     1081 ttaggtcacg tcggactgga ccccagccgt attgctgctc aggttgtgac gggcgtcggg
     1141 tttatcggtg caggtaacat tctcgttcgt aaccaaaata ttgtaggtct gacgacggca
     1201 gcggatatct gggtgaccgc cgccattggt atggttattg gcagcggtat gtacgaactg
     1261 ggtatttatg gttcagtgat gaccttgctg gtgctggaag tcttccatca attaaccttc
     1321 cgcctgatga acaaaaatta ccatttacag ctgacattag tgaacggtaa tacggtatca
     1381 atgctcgact ggtttaagca gcaaaaaata aaaacggatt tagtttcgtt gcaggaaaat
     1441 gaagatcatg aagtggtcgc cattgatatc cagcttcatg ccacgacgtc gatagaagat
     1501 ctgttgcgat tattaaaagg tatggcaggg gtgaaaggcg tttcaattag ctaatctgta
     1561 tagttattta ttcgcaggag taccagtgcg gtactcctgt tggttttaat gatcgaggac
     1621 gatcaggggt gaatggttgg caggatcttt aagtacggta atatccgcgc cgtaaagcat
     1681 ggtcagcgcc tgctgtgtca atacgtcctg tggtttacca tttgcgataa cctttccttt
     1741 ttgcattaat acaatacggt ctgcataacg cgcggcaaga tttagatcgt gcaatacgca
     1801 gcaaacattg aactgccgtt cgtgaactaa ctggcgtaat aaacggaaca agtgctgctg
     1861 atggtgaata tccagcgccg aagttggttc atcaagaaat aaccatttcg gtgagggagt
     1921 tggttcccag agttgcacca gcaggcgcgc taattgtacg cgctgttgtt caccaccgga
     1981 taattgccga tagtcgcggt ttgctaacgc ctgacagtca cataacgcca ttatttgtgc
     2041 tgtttcatta tcctgattac cagtgcgatg cggatgccgc cccatctgga taacttcttg
     2101 tacgctgaat ggaaacgcca tgtggctatt ttgacgcatc acggcccggt gtttcgccag
     2161 ttcagtaatc gaccattcat ttaatggctt attaaacaac cgacattcgc cgctatcagg
     2221 ttgaagatag ccggttaact ggcgaagcag cgtagatttt cccgcaccat tagggccgag
     2281 aatggcaaca atttccccac cgggaaaagt cagcgagaca ttatcagtca gacgccgccc
     2341 ctgaaggcta taaaccagat tctgggcgct gatcatgtga tcctccggtt gcgcagaatc
     2401 aaccacataa aataaggacc accaagcagg ctggttaata atcccacggg catttctgct
     2461 ggctgtacca gcgtgcgggc aagcgtgtct gccatcagca ataaacaggc tccggcgagg
     2521 gcggagcaag ggattagcca gcggtgattt gctccggtag tcatgcgaat cagatgcggg
     2581 atcaccaggc caataaagcc gataatgccg cttaccgata cggcggcacc aacgagcagt
     2641 gagctcacta acagtaattg ctggcgttta cgcttaacgt tcacgccgag gtaatgggct
     2701 tcttcatcac cgagctgcag taaattcagc gttccggcga gacaagttgt tgcgataatg
     2761 gccggtagga tgaatgaact ggcaaccaat aacgtcggcc attgcgcctg tccaagattg
     2821 cccatcatcc acaatgtgag ttgtcgtagc tgctgctcat cgccgatata gctcaggatg
     2881 ccgatcgctg cgccgcacag ggcattgatg gcgataccgg cgagtaataa ttgcatcatg
     2941 ctgccatcgc gatggcgctg cgtgatgaga aaaatgatgg tgcagaccac taaacttccg
     3001 gcaatggcaa acaccatttg ctcatagagc accagcacca ctgggaagga gaagggcagc
     3061 acaatagcaa cgccaaccat taatgcagaa ccgctactga cgccaagtaa tccagggtca
     3121 gccattgggt tgcgaaaaag cccctgcata attaccccgg cggttgccaa cgctgcacct
     3181 accagcaccg ccagcaaaac ccgtggtaga cgaatattga gccagatatc gcggtattcc
     3241 tcatcaaaaa gggcatcaaa actgatcttt aacgctccct gactggcacc aaacagcacc
     3301 agagctagca ataatagtga tagccccata aagaccctgg cggaagagag tgaatccttg
     3361 agcacgcaaa actccgttac gacattacgg cgcggacatg ccgcgccggg ggatcacgcc
     3421 ggtttcaggt ccggttcaat cagactgtag acctgctggt taagtgctgg cgcatttgcc
     3481 agttcatgga tatgtgcggc gacatcagca cggttaataa aaccatgaca ctcctgattt
     3541 tgtatccgtt gtgctttgcc cgttgccgca ccatcaagca gaccgccagg gcggagaatg
     3601 gcataatcaa gctggctggt ttgcaaccag ctttccgcca gcgttttttc ccgtaccgcc
     3661 tgaccaaacg cagcctttgc ccgctctgat aaaaacggcc agctatcgcc gcatcccagt
     3721 gacgtcacca gaatcatgcg tgtgatgcct gctttctcgg cctcgtcaat cactgttctg
     3781 tgcgccagat aatcctgagc tccgcccatt gttgagatga taagcgcatc cgggccagct
     3841 gcgcggcaag cagccgcgac tacgctggca tcacaggcgt cgcctgtaaa aacctgtacg
     3901 ccttgttgcg ccagtttcgt ggcggcatcg gcatgacgaa tgacagcaac gaccggacgt
     3961 tgttccgcca gcgccagttc aagcgttctg gcacccacac cttttccgcc tgcgccaaag
     4021 agtagccacg gtgtcatcag gcgtgttcct ttaacgatgc tgccagagca tggaaggcac
     4081 tgacttgttc gctcaggagc tgacgatgat catccctacc gaggaagatt ttcagcattg
     4141 cgctgccttc cttattgaaa aagagaatag aggcggtatc catgcccata aatttacgtt
     4201 cgatcaaggc aatatgcgtg cagttttctg ctttgatatg cccggacatg ccgtgctttc
     4261 cgcgtaggtt gaaatagccg tggcgatgga aacccgaagg cagttcgccg ctaaattcga
     4321 gtattacatc ggtagtatgt actaacgtgg tgacgttgcc ccattcgcag acggtatccc
     4381 agacagtatc gaatttatca cctgacacaa cagtcggaga gggcagattt ttcactacct
     4441 ccagcagcgt ggtgttgtat tgttcggcta tcgcctccag cgtaccgtct ggctccgttt
     4501 tcaaaaattc ctgtaacgag acatggctca taaatcactc ctgttatggt ttttcaagcg
     4561 caattgctgg cgcattaagt acctgaatca gttcattaag agactgcaaa atgttgcttg
     4621 cccagaaacg accttcattg gtcaaccgca gacacgttga cgcatcgcgg cttaagcctt
     4681 tttggtgcca ctgggccagt aacggcgcga gtttttcggc atgtggcgta agttcgtcca
     4741 gcggtacacg cgccgtttca atgcccgact gcaaggtatg acgccactgc gcgttacgtt
     4801 cggcgttacg catgatcatc atcagcggtt ttttacctgc tgcgacggat tcatgccagg
     4861 tctgaagatt gcgttcgttc atccaggagt aaccattaat cgaaccgccg gctccggaac
     4921 caaaggccag acaatcggca ccttgtttaa tcagcaggtt atagagattg cgttcgcgtg
     4981 tggtacggcc ccagtggctg ttactgatgc agcgccaacc ggcatcgtcc ataaaatcac
     5041 acccttgcag gtaaagatcg cgacgttctg ccggagaggg cacggtagta cgcccatttt
     5101 ccacggcttt gcccagcggt gtattgggca ggacattgag cgcatagaga tcgacgccgt
     5161 cgagaccgat atcgcgggca atagccagat cttctcccca ggtttgcgca tcctgacccg
     5221 gcagaccaaa cagcaggtca cagaccaccg cggcacggtc gcgtttgacc aggctttcca
     5281 taaacgcaat agcggttggg ccatccgagg tgcgggccat tttcttgcgg attttgctgt
     5341 taaacgactg aataccaatt gagaagcggt ttgctccagc atcaaggcaa gcatcgattc
     5401 gctcagcgtc aaaattcagt acccggcctt caatagtgat ttcacaatca ggggccagtg
     5461 gcagcttttc tcgtaatgtg gtgatgattc tggccaaatc gtgtgctgaa agcgcggaag
     5521 gcgtaccgcc accgaaatag accgcgtgga tgggggcaga ctgatgcaat acgctgtctg
     5581 cttccatttc tatttcacga atcagggcgt cggtataatg agcacacgca tcgtcattaa
     5641 aacggttctg ataaaaacca caaaacgtgc agtgtgtcgc acaaaaagga atgtgtagat
     5701 aaaccagtcg cttacgtggt gataccgttt gattgagcat ctcttgccag gtctgtgtca
     5761 gttgctcttt ggcaactgga atggccccac gaaacggcat catggcgcgc ctgtctttaa
     5821 atggctgatc accgtctaaa gcaaaatgtg gcgtgagatc cagggtgtta tttgtgttca
     5881 ttcgtctgat ttaaatccat ttattgcggc caaagctgct ggtgtaaaga ctcaacgaca
     5941 tcagcaatac gcggacccat tcccagaatc aggttttgat caacggtgat aatgcgctgg
     6001 tttttccatg cggcagtgtg ggtaattccg gcaatagaac gtagccgatt aatatcgcca
     6061 tcgaccattt gtgaggtgac gacaatcact tcagggttag ccgcaatcag cgattccgcg
     6121 ctgtagcttt tgtactgctg gtgggtagcg acgttttctg ctccggcaag cgaaagaatg
     6181 gcgtccgcga cgctgccttt accggcaacc tgtggcgcac ttccgcctgc ggagagaata
     6241 aacatggctt taaccggggc ttttttggcc gccacgtttt gctgtactcg ctccaggcgt
     6301 tggttgatct gtgtcaccag cgcctcgcct tgttcaggaa cctgtaacgt ttttgccagt
     6361 tggcgaatgt tggcgtacat ctgctcaagg gtggctggta cacgcggcag agtgacgacg
     6421 ttgacttttt gcgcccgcag ttggtcgagc acaatttgcg gtcctgcatc ctgccaggta
     6481 attacgctat ccgggcgaag tgacaaaatg ccttcgctgc tgagttgttt ccagtaacca
     6541 atatgcggca gtttggcggt ttctggtgga taagatgtcg tttcatcgac accaaccacg
     6601 cgttcgccag cgcccatcgc gtagattagc tccgtcagcg atcctcctgc gaccacgata
     6661 cgttcggtag ccgttacgct aaaagcccag ctaagagcca gtactgccgt aaacaatttt
     6721 tttcgtttta ccgcggtctt cgcggcagag gccacggctg aaatacataa cgtcagtggg
     6781 gaaaaaagaa acggcctggt gatgatcctt ggcataatat gagaactctt atcaaattga
     6841 gaatgatcat gattatcatt tgagttatat atgaaataca accgtcgatt caatacgcac
     6901 ggcgttacaa gcgtatttat ttttgatttt tatcaagatg cttcgtcagc taacgttctt
     6961 gttaccgact aaatttcagc gcgggaaaaa gctgagaacc cagagatatc gcggcttgca
     7021 gagcaaagag aagagaaaat tatcactgtt aacgacaaag tttagtgatg aggtcatggg
     7081 ggaataacca taaaaacaaa gtggtttcca gggcgcagga taagtttcat aaccccctgt
     7141 ttattgtgtt aatggcggtt aaaaaaaagc ttgatcataa aaaattgatc gataataatt
     7201 ctcattatca cgtttgcatt taacaaaata cggccatatg gataacaaag ccatatcatt
     7261 tacaaggggt aacgcacagc gctcttcact cctgatgggg catatcggtt tgcctgacaa
     7321 agagggcgtg cttttgcgta tttgccgaga taccaggcaa tttcatggga tataaacgcc
     7381 tgtaaactca cccggcgaat attatcgctc tgaattttaa ataaatttat ttttcgccag
     7441 ccaccccggt attaacccag gcagccagca ttttttattt tcctcatgga gaatcgctat
     7501 gtcacgtccg caatttacct cgttgcgttt gagtttgttg gctttggctg tttctgccac
     7561 cttgccaacg tttgcttttg ctactgaaac catgaccgtt acggcaacgg ggaatgcccg
     7621 tagttccttc gaagcgccta tgatggtcag cgtcatcgac acttccgctc ctgaaaatca
     7681 aacggctact tcagccaccg atctgctgcg tcatgttcct ggaattactc ttgatggtac
     7741 cggacgaacc aacggtcagg atgtaaatat gcgtggctat gatcatcgcg gcgtgctggt
     7801 tcttgtcgat ggtgttcgcc agggaacgga taccggacac ctgaatggca cttttctcga
     7861 tccggcgctg atcaagcgtg ttgagattgt tcgcggacct tcagcattac tgtatggcag
     7921 tggcgcgctg ggtggagtga tctcctacga tacggtcgat gcaaaagatt tattgcagga
     7981 aggacaaagc agtggttttc gtgtctttgg tactggcggc acgggggacc atagcctggg
     8041 attaggcgcg agcgcgtttg ggcgaactga aaatctggat ggtattgtgg cctggtccag
     8101 tcgcgatcgg ggtgatttac gccagagcaa tggtgaaacc gcgccgaatg acgaatccat
     8161 taataacatg ctggcgaaag ggacctggca aattgattca gctcagtctc tgagcggttt
     8221 agtgcgttac tacaacaacg acgcgcgtga accaaaaaat ccgcagaccg ttgaagcttc
     8281 tgatagcagc aacccgatgg tcgatcgttc aacaattcaa cgcgatgcgc agctttctta
     8341 taaactcgcc ccgcagggta acgactggtt aaatgcagat gcaaaaattt actggtcgga
     8401 agtccgtatt aatgcgcaaa acacggggag ttccggcgag tatcgtgaac agataacaaa
     8461 aggagcaagg ctggagaacc gttccactct atttgccgac agtttcgctt ctcacttact
     8521 gacatatggc ggtgagtatt atcgtcagga acaacatccg ggtggcgcga cgacgggctt
     8581 cccgcaagca aaaatcgatt ttagctctgg ctggctacaa gatgagatca ccttacgcga
     8641 tctgccgatt accctgcttg gcggaacccg ctatgacagt tatcgcggta gcagcgacgg
     8701 ctacaaagat gttgatgccg acaaatggtc atctcgcgcg gggatgacta tcaacccgac
     8761 taactggctg atgttatttg gctcatatgc tcaggcattc cgcgccccga cgatgggcga
     8821 aatgtataac gattctaagc acttctcgat tggtcgcttc tataccaact attgggtgcc
     8881 aaacccgaac ttacgtccgg aaactaacga aactcaggag tacggttttg ggctgcgttt
     8941 tgatgacctg atgttgtcca atgatgctct ggaatttaaa gccagctact ttgataccaa
     9001 agcgaaggat tacatctcca cgaccgtcga tttcgcggcg gcgacaacta tgtcgtataa
     9061 cgtcccgaac gccaaaatct ggggttggga tgtgatgacg aaatatacca ctgatctgtt
     9121 tagccttgat gtggcctata accgtacccg cggcaaagac accgataccg gcgaatatat
     9181 ctccagtatt aacccggata ccgttaccag caccctgaat attccgatcg ctcacagtgg
     9241 cttctctgtt gggtgggttg gtacgtttgc cgatcgctca acacatatca gcagcagtta
     9301 cagcaaacaa ccaggctatg gcgtgaatga tttctacgtc agttatcaag gacaacaggc
     9361 gctcaaaggt atgaccacta ctttggtgtt gggtaacgct ttcgacaaag agtactggtc
     9421 gccacaaggc atcccacagg atggtcgtaa tggaaaaatt ttcgtgagtt atcaatggta
     9481 atcatctgcc ccgatatttc ggggcattta tctggaagga agagagacaa tgaaccacta
     9541 cacacgctgg cttgagttaa aagaacaaaa tcccggaaag tacgcgcgtg acatcgcagg
     9601 tttaatgaat attagcgaag cagaactggc atttgcgcgc gtcacgcacg atgcgtggcg
     9661 gatgcgcggc gatatccgtg aaattctggc ggcgctcgaa agtgttggcg agaccaaatg
     9721 tatttgtcgt aatgaatatg cagtccatga gcaagttggt gcgttcacaa accagcattt
     9781 gaacggacat gccggattga tcctcaatcc acgcgcgctg gatttgcgtc tgtttctcaa
     9841 tcaatgggcc agtgtttttc acattaaaga aaatacgact cgtggcgaac gccagagtat
     9901 tcagttcttt gatcatcagg gcgatgcatt actaaaagtt tatgccaccg acaataccga
     9961 tatggcggca tggagtgagc ttctggcacg gtttatcgcc gatgagaata tgccgcttga
    10021 gttaaaagcc gttgatgcgc cagttgttca aacgcgagcc gatgccagtg tggtcgagca
    10081 agagtggcgg gcgatgaccg acgttcatca gttttttacg ttgctcaagc gccacaacct
    10141 gacgcgccaa caggcgttca atctggtggc agatgatttg gcctgcaaag tatccaacag
    10201 tgcgttggcg caaattcttg aatctgcaca gcaggatggc aatgaaatca tggtgtttgt
    10261 tggcaaccgt ggctgcgtac agattttcac tggtgtggta gaaaaagtgg tgccaatgaa
    10321 aggttggctg aatattttca acccgacgtt tactcttcat ctattagaag agagcattgc
    10381 tgaagcctgg gttacccgta aaccgaccag cgatggctac gtaaccagtc tggaattgtt
    10441 tgcccatgat ggtacgcaga tagcgcaact ttatggtcaa cgtacagaag gcgaacagga
    10501 gcaagcgcaa tggcgtaagc aaattgcttc gctgataccg gaagtcgtta ctgcataacg
    10561 tataattaac gggaggtctg gctatttatt gctgggcctc tttatattga tcaatcgttc
    10621 acaccagata atcaatatgc tgatggcgta ccaactcatt gatccgctta agattgagga
    10681 tcatcatgat attgtgttta tggcagtaga acgtttttag cgatattttc agtttctcca
    10741 gaatatcttc cgttgtcatt tgattcgcaa tgcagctcag aacctgatgc tgcttgcgcg
    10801 aaagggacac atccgcttca ggcacagagc agtaactgcc attgatgagt gcgacaaagt
    10861 cgatgatggt gatagttcgc ggaacaaagg tcaccggtac tttccccagg cagcgtttaa
    10921 tattaaaggg agagaaaata atcacatgga caggttttaa tcgttcaatc tgagtgagca
    10981 aattagaatg aaatacatta ttcattaatg tatcaataat gacgaaggca tgctgatgca
    11041 acattacgtc gatctcttct tcgttctgta tatgaacgac attaccttta ctcagaatgt
    11101 ttttcatcgc agtgaagaac atcgtatccc tggtaattat aagaaacata accgactcct
    11161 ggtgcggaca ttaactttat aatcagtttg ccaaaggtaa ttaacaggac agggcttata
    11221 ataatagtta gctggctgtg tattaatttg atgtaatgca tgatgcagat gctgagaaag
    11281 catctgcatc tctcggtggt gttatttgac cagctcaggt gttacttgac tcaccgcatt
    11341 ggtgtagtaa ggcgcgcccc agcccggttc cggccagaat gcaccatagc cgtaatccca
    11401 caggttatag gtggtattaa ccacttctct caaatgccac acctggatgc cctgcatatt
    11461 cacttccagg aagttatacg ggactttatt gataaagcca ggttgttcac cctgaatggt
    11521 accgaggatg gtaacaaagt gattacgata gttcactgga tcaaggaagc cgctttgtct
    11581 ggcgagcagt cggccctgat agttggcttc aatatcaggc ttcgcatagc tatccaacgg
    11641 taatacagcg atttctaaca acgtatccgt tttgccattg ataacgttga taaccttccc
    11701 gccaaagcgc gcttgttgac caacatataa ccccggctgg ttatgaacag caacaaaact
    11761 tttttgaata tcaggttggt tattgccttt gatattttgc ggaattgaac tacatgcggc
    11821 aagcaaaaat gaaaggctga ggatgagtgc accttttgtc atg
//
LOCUS       DAZKGF010000149        11846 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZKGF010000149 DAZKGF010000000
VERSION     DAZKGF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.149"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            298..798
                     /gene="tssB"
                     /locus_tag="ACHOQV_003900"
     CDS             298..798
                     /gene="tssB"
                     /locus_tag="ACHOQV_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP9632193.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKVDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            857..2395
                     /gene="tssC"
                     /locus_tag="ACHOQV_003901"
     CDS             857..2395
                     /gene="tssC"
                     /locus_tag="ACHOQV_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP9632194.1"
                     /translation="MSVQQEYATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVKIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDVNLSLVSQMP
                     KAKA"
     gene            2415..3752
                     /gene="tssK"
                     /locus_tag="ACHOQV_003902"
     CDS             2415..3752
                     /gene="tssK"
                     /locus_tag="ACHOQV_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP9632195.1"
                     /translation="MKICRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     SNVLFDRDALTLGKLKTQAVRLRFADGTLIDSDVADILPPACDLRTLEGGSAIVLLAL
                     PLAHGNGGNLGQGEQAERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLTLNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLQEEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRS"
     gene            3749..4414
                     /gene="tssL"
                     /locus_tag="ACHOQV_003903"
     CDS             3749..4414
                     /gene="tssL"
                     /locus_tag="ACHOQV_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFP9632196.1"
                     /translation="MKKETDINIDVLLRDTFLTVVELRQGTTVRHGIELYRHCQRQVE
                     LVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKNPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPVAPERDQLISTLNGLVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            4427..6079
                     /locus_tag="ACHOQV_003904"
     CDS             4427..6079
                     /locus_tag="ACHOQV_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9632197.1"
                     /translation="MRNTLKQAIVLWGMVLLLVLWWVFVSPSGVLRWAGAAVIVLAVA
                     ALLIYRRRQTWTEMTGDAGLLSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVPDEEQLVAQVERLLTLRPAWASQLAVAYTVMPGMYRDTAVLTGQLRRFAHSMAIVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEVQVATSAETTMPAQWIAQSGAQE
                     RSQRLSYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPVEEFITKARHLSVLKDDATMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRTIRDWRPPEQKMEVTASLQVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPLGRAVNRRVEISLVPRSDACQDVK"
     gene            6137..6628
                     /locus_tag="ACHOQV_003905"
     CDS             6137..6628
                     /locus_tag="ACHOQV_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP9632198.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVPTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6820..9465
                     /gene="tssH"
                     /locus_tag="ACHOQV_003906"
                     /gene_synonym="clpV"
     CDS             6820..9465
                     /gene="tssH"
                     /locus_tag="ACHOQV_003906"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP9632199.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRSVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLHPLLDAQSDECPA
                     RQPQVTEPLTSAQPESATTDVPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRIAEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAAQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     VQQIMTLRQEGTDSSELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLKD
                     EQSDLLSMEQSLENRVVGQRPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRTIVEMKLAQVVR
                     RLHQHYGLETEINDSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAAHR
                     KPAQLRLGWDEEDGIVLEFATEEMQ"
     gene            9477..>11846
                     /locus_tag="ACHOQV_003907"
     CDS             9477..>11846
                     /locus_tag="ACHOQV_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP9632200.1"
                     /translation="MGIQNFDHSHHKLKIRGLKSPVDVLTFTGREQLSAPFRYDIEFT
                     STDKTIEPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQ
                     YGEDDLTFISRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADN
                     FLQQGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIKVQGDDAPAVFR
                     KGVLITGVTTSAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDMWGVVRAKKGIFISADAQDNAQGQVREMAP
                     AMAILNGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLNQAAILLSAPKGVAI
                     ASGEHLQLAAAKNVIANAGNNADVGAVKNMFIGVGQALSVFVRKAGIKLFANKGAISV
                     QAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTQGEYNV
                     KAGYYGRKPKAKLTPELMAFPVISAKFDEQVRVIDDDGKFIANVPYYIED"
BASE COUNT         2752 a         3272 c         3409 g         2413 t
ORIGIN      
        1 tcttaatagg ctctcatact acccaatttc ttaaaagtgc aaatttgtga agcttaccct
       61 gttattattt taagtttcat gccaaattct ctcaccagat aatgccaccc tctcccgaaa
      121 aataatcaag aggccaaaca atatctaaaa tgatacaact gtatctattc ccctgaaaaa
      181 tacattattc atttgtatat tttcctcatc attgcttttt atttaaatca tccgataatc
      241 ccctgaatat aattatgtca ataaccatca gaaaaagtgg atgatgagga aaaggatatg
      301 gccgacagtt tccagaatga agttcccgcc gctcgtgtaa atatcaagct tgatctgcat
      361 acaggcaatg ctaaaaagaa agttgaactc cccctcaagc ttcttgccgt aggcgattac
      421 agtaacggaa aagagcaacg tccgctgtcc gaacgggaca aggttgatat caataaaaac
      481 aacttcaaca gcgtcatggc tgagttttcg cctgcggtta atttaacagt agaagatacg
      541 ctaaacggaa acggtaatga acaaaatatc gcgcttgaat ttaaaagcct gaaggatttt
      601 gaacccgaac aggtagcaaa aaatattccg caacttcgtg tattactcgc tatgcgcaat
      661 ttactacgtg acctgaaatc caatctgctg gataacgcaa cgtttcgccg tgaactggaa
      721 aatatcctga aagacccgac gctcagttca gaactgcgtg atgaactggc gaaaattgcg
      781 ccgcaggaga acgtttaacc cgaacgcgta ttcaggttaa accggagaag aaaggatccc
      841 taaaaaggaa atgctgatgt ctgtacaaca agaatatgcc acctctgaaa ctgcaacact
      901 caccaccact gagtccggcg gcgtttatca gtccctgttt gataaaatta atttaacccc
      961 ggtctcttcc attcaggaaa tcgatttatg gcaaaacagc gaaacgctgg ccgatgcttc
     1021 acccgatgag cgcgtgacgg cggcgatcca cgttctgctt tcctgtctgg cgaaatcagg
     1081 cgaggacgtg gttaaactcg acaagagcct gctggatttt catatcgacg atttggatca
     1141 gaaaatcagt aaacagcttg atgcagtcat gcaccatccg gaattccaga aagtcgagtc
     1201 gctctggcgc ggcacatggt tcgtcgtaca gcgcaccgat tttcgcaaaa atgtcaaaat
     1261 tgaactgctg gatatcagta aagaacatct gcggcaggac tttgatgatt ctccggaaat
     1321 cattcaaagt ggtttatatc gccatacata cattcaggaa tatgatacgc cgggtggcga
     1381 acctgtagcc tcattaatat ccagctatga attcgataac agcccgcagg atattgccct
     1441 gctgcgtaat atttccagag tctctgctgc atcacatatg ccctttatcg gttccgtcgg
     1501 accgaagttc ttccttaaaa actcgatgga agaagtcgcc gcaattaaag atatcggcaa
     1561 ctattttgac cgcgcggaat atatcaaatg gaagtcgttc cgcgataccg acgacagccg
     1621 ctacgtggga ttagtgatgc cgcgcgtgct gggccgtctg ccctatggcc cggacacggt
     1681 gccggtacgc agctttaact atgtggaaga ggtcaaaggc ccggatcacg aaaagtacct
     1741 ctggacaaac gcctcgtttg cctttgccgc caatatggtg aagagttttg tgaataatgg
     1801 ctggtgtgtg cagatccgtg gtccgcaggc gggtggcgca gtagccgatc tgccgatcca
     1861 tctttacgat ctcggcaccg gcaatcaggt caaaattccg tccgaagtga tgatcccgga
     1921 aacccgcgag tttgagttcg ccaaccttgg ctttatcccg ctctcttatt ataagaatcg
     1981 cgattacgcc tgcttcttct cagcgaactc tgcccagaaa ccggcgctgt atgataccgc
     2041 tgacgccacc gccaacagcc gtatcaacgc ccgtttgccc tatatcttcc tgctgtcccg
     2101 cattgctcac tacctgaaaa tcattcagcg cgagaatatc ggcaccacca aagacagacg
     2161 cgtgctggaa ctggagctga acacctggat ccgcacgctg gtgacggaaa tgaccgatcc
     2221 gggcgatgaa cttcaggctt cgcatccact gcgcgacggg aaagttatcg tcgaggacat
     2281 agaggacaat ccgggctttt tccgcgtcag actctttgcc gtgccgcatt tccagatcga
     2341 agggatggac gtcaaccttt ctctggtttc ccagatgcca aaagcaaaag cctgatcggg
     2401 cggtggtgtg atgtatgaaa atttgtcgac cactgtgggc tgaggggaca ttcctgtctt
     2461 ctcagcagtt tcagcagcag gcccgctggg aagcgttcag caatgacagc attgcgcagc
     2521 tttgtattcg ccatccgtgg gggatctcaa acgtgctctt tgaccgggat gccctgacgc
     2581 tcggaaagct gaaaacgcag gcagtacgcc tgcgttttgc tgatggtacg ctgatcgatt
     2641 cagacgtggc ggatattctg ccgcctgcct gtgacctgcg cacgctggag ggcggcagcg
     2701 cgattgtttt gctggcgctg ccgcttgcgc atggtaacgg cggtaatctc ggtcagggcg
     2761 agcaggctga aaggccgctg cgctatcgtc aggaatggca aaaggtgcag gatatctacg
     2821 gcagtgacag tgaagatatg gccgttgagc gccatgcact gtccctgcgc tttgcccatg
     2881 ataacaatca ggattacatc acctgtccgc tggcgcgtct ggtgcgggat gttcagggca
     2941 actggacgca ggatgagagc tatattccgc cgttactgac gttaaacgcc catgacgggc
     3001 tggtacagcg cctggacacg ctgctgttgc agctacgggc gaagtgccag cgcctgatgg
     3061 cgatgcgccg ggaaagcaac cagcgcatgg cggacttcgc cgtcgccgat gtctctctgt
     3121 tctggctgct taacgccctg aattccgccg agccggtgct gagcgatttc ctgcgctacc
     3181 cggctgtcca ccccgaactg gtctggcggg agctggcgag gcttgccggt gcgctgctga
     3241 cgttttccct tgagcataac gtcagcgccg ttccgcccta tgtccacgaa tcgccgtcca
     3301 ccgtctttcc gccactgttc tccctgctca gcgagctgct ggaagccagc ctgccgtcgc
     3361 gggtgatcgc gctggatctg gcgtccctgc cgggcaaccg ctggaaagcc gacctgcacg
     3421 atccgcgcct gcaggaagaa gccgattttt acctgtccgt tcgttcctca ctgcctgcgc
     3481 atcaggtact gcaccagcta ccgctggtct gcaaaatcgg tgcgcctgat gacgtcacgc
     3541 tgcttatcaa cgtggcgctg aacggtgttc cactggtgcc actcacctcg gttccggcgg
     3601 cgctgccgct gcggctggag aaccagtatt ttgcgctgga tatgcacagt gacgcggcta
     3661 aatcgatgct ggagtccggg tgctgcatga tttatgcgcc gggaacaatg ggcgacctga
     3721 aacctgaact gtttgcggtg ctgcgctcat gaaaaaagag acggatatca atatcgacgt
     3781 gctgctgcgc gacacgtttc tgacggtggt ggaactgcgt cagggaacca ccgtgcgtca
     3841 cggtatcgag ctgtatcgcc actgtcagcg gcaggtggaa ctggtccgcg aacgtctgaa
     3901 agatgccgga ttcagtcggg agaacgtgga gcacataact tacgcgcagt gcgccctgct
     3961 ggacgaaacg gtgctcagcc gtagtggcat ggacgacggt caggccatct ggatgaaaaa
     4021 cccactccag tcgcatttct tcaacactct gcaggcaggc gagctgctgt acgaacggat
     4081 gaagcaggtg ttgcaggagc ccgcccccgc gcaggcagtg ctgacctgct ttcaccgtgt
     4141 cctgctgctg ggatttcgcg ggcgttatca ggatccggtg gccccggagc gtgaccagct
     4201 tatttcgacg ctcaacgggc tggtcgcgcc atttggcgtg ctgccggaaa cggcggtact
     4261 gaacgtgccg ttatcccccc gtcagcatcc tttgctgcat tcgccgtttt tctggctggt
     4321 aacgctgacg ctgctactgg ccggtgtctg gtggggactg caccactggc tgaacgtgct
     4381 ggtggatgaa ttgctgccgc aaagcctgcg gtaaagggag ccgtgagtga ggaacacgct
     4441 gaaacaggcc atcgtgctgt ggggaatggt gttactgctg gtgctgtggt gggtgtttgt
     4501 cagtccgtct ggcgtactga gatgggccgg tgcagcagtt atcgttctgg cggttgccgc
     4561 attgttgatt taccggcgca ggcagacgtg gacggagatg accggcgatg ccgggttgtt
     4621 atcgctgccg ccggaaacgt accgacaacc ggtagtgctg gtctgtggcg atatgtcagc
     4681 gcatctgttc actgacagcc cggtccggca ggtttcagaa gggctgtatc tgcatgttcc
     4741 cgatgaagaa cagcttgtgg cgcaggtgga gcgattactg acccttcgcc cggcgtgggc
     4801 atcgcagctt gccgtggcgt ataccgtcat gcccggcatg taccgggata cggcggttct
     4861 gaccggacaa ctgcgacggt tcgctcacag tatggcgata gtgcgtcgcc gggcgggcgt
     4921 aaacgtaccc tggcttctct ggagcgggct gtccggctcg ccgttgccgg agagagcgaa
     4981 ttcaccgtgg tttatctgta ccggcggcga agttcaggta gcaacatccg cagagaccac
     5041 catgcccgcg cagtggattg cacaatccgg cgcacaggaa cgcagtcagc gactctctta
     5101 tctgctgaaa gccgaaagcc tgatgcaatg gctggatctg aatgtgctgg cggaactgaa
     5161 cggcccggag gcgaaatgtc caccactggc gatggccgtg gggctggtcc cctcgttgcc
     5221 tgcggtggat aacaacctat ggcagttgtg gatcaccgcc agaaccggcc tgacgccgga
     5281 tatcgcggac accggcacag acgatgcgct gccattcccg gatgccctgt tacggcggtt
     5341 gccgcgtcag tcgggcttta ccccgctgcg acgggcctgc gtgaccatgc tgggcgtcac
     5401 caccgtggcg ggtatcgccg cgctgtgcct gtcagccacg gcaaatcgcc agttattacg
     5461 gcaggtcggt gacgatctgc accggtttta tgccgtcccg gtggaggaat ttatcaccaa
     5521 agcccgtcac ctgtcggtgc tgaaagacga tgcgaccatg ctcgatgggt attaccggga
     5581 aggagaacca ctgcgcctcg gtctggggtt ataccccggc gaacgcatcc gccagccggt
     5641 attacgcacc attcgcgact ggcgtccgcc tgaacaaaaa atggaggtga cggcttcgct
     5701 tcaggttcag accgtgcgtc ttgacagtat gtcgctgttt gacgtcggac aggcccgcct
     5761 gaaagacggc tcgacaaaag tgctggtgga cgcactggtg aacatccggg caaaaccggg
     5821 ctggctgatc ctcgtggccg gatataccga cgccaccggc gacgaaaaaa gcaatcagca
     5881 gttatcgctg cgacgtgccg aagcggtgcg taactggatg ctgcagacca gcgacatccc
     5941 ggccacctgt tttgccgtac agggactggg tgagagccag cctgcggcga ccaacgatac
     6001 gccactgggc agggcagtca accggcgcgt cgaaatcagt cttgttccgc gttctgacgc
     6061 ctgtcaggac gtgaaataaa acataccgcc ggaagaaggc ggtgcttcaa tcacactaac
     6121 aaggagagta attctcatgg ctattcctgc ttatctctgg ctgaaagatg acggcggcgc
     6181 agatatcaaa ggttccgtgg acgttcaggg gcgcgaaggc agcatcgaag tggtggcgtt
     6241 ggatcacgat gtgtacatcc cgaccgacaa caacaccggc aaactgaccg gtacccgtac
     6301 tcacaagccc tttacgttta ccaaagaaat cgatgcgtcc agtccgtatc tctacaaagc
     6361 tgtgcccacc ggtcagaccc tgaaaacggc agaatttaag ttttaccgca tcaacgatgc
     6421 cggtcaggaa gtggagtact tcaacatcac gcttgataac gtcaagctgg tcagagtcgc
     6481 tccgctgatg cacgacatca aggatccttc cagagagaag cataaccacc tggaacgtat
     6541 tgagttccgc tacgagaaaa tcacctggac ttacaaagac ggcaacatca ttcattccga
     6601 ctcgtggaat gagcgtcctt ccgcctgata actgcggcag acaggaaact gtctgccttt
     6661 ttgttgtgtg ctttgtccag tgccgcaggc accggtactg agcaaagcat acccacaatc
     6721 tggtgaaacc tgatgggcct gtgtgaggta acggctatgg gcggtagcgt gcctgaacgc
     6781 ggatcgcagg aatacagaac acgataagga aacccttcta tgacaggaaa tcaccccgcc
     6841 gcgctgctgc gtcgccttaa cccatactgt gcacgggcgt tggacgctgc cgcctcactg
     6901 tgtcagaccc gcgcccatgc ggagataacc attgaacact ggctgctgaa actgctggag
     6961 cagggagaag gcgatatcac ggtgattgcc cgtcgctatg aatgggatat cgacacgctc
     7021 tggcagtctc tgctggcgca tctggacacc ttaccccgct cggtccgcga acgtcctcaa
     7081 ctttctgaac cgctggcagc gcttatcaga caggcgtggc tgatagcgtc actggaaggt
     7141 gacgatccac aaatccgcag ccagcacctg ctgatggcgc tgacagaaaa accgatgctg
     7201 cccgcctgta atgacctgtg ggtattgctg agtctgagcc gcgtgcagct tgagcggctg
     7261 catcccctgc tggatgcgca gtcggatgaa tgtccggcac gtcagccaca ggtcaccgaa
     7321 ccgctgacat ccgcgcaacc agagtcggca acgacggacg taccggcaaa aacgctgacg
     7381 gggaaacagg atgacgccct gctggcggtg cttaaccgct ttaccgaaga cgtgacggaa
     7441 aaagcccgta gcgggcgaat cgacccagta ttcgggcgcg acaccgaaat tcgccagatg
     7501 gtcgatatcc tctcccgtcg ccgcaaaaac aacccgattc tggtgggaga accgggggtg
     7561 ggcaaaaccg cactggtgga agggctggcg ctgcgtatcg ccgaaggcaa cgtgcccgac
     7621 agcctgaaaa cggtgcatat ccgcacgctg gacctcggtc tgttacaggc tggcgcgggt
     7681 gttaaaggtg aatttgagca gcgactgaaa aacgtgattg atgcagcaca gaaatcgccg
     7741 gagccggtac tgctgtttat tgatgaagcc cataccatta tcggcgcggg taaccaggct
     7801 ggcggcgcgg atgcggcgaa cctgctgaaa ccggcactgg caaggggcga actgcgcacc
     7861 atcgcggcga ccacgtggag cgaatacaaa cagtattttg agcgcgacgc cgcgctggag
     7921 cgccgcttcc agatggttaa ggttgacgag ccggatgatg acaccgcctg tctgatgctg
     7981 cggggactga aggcccgtta tgcacagcac cacggcgtgc atatgctgga cagcgccatt
     8041 cagaccgccg tgcgcctgtc gcgccgctat ctgaccggac gccagctgcc ggacaaggcg
     8101 gttgatttgc tggataccgc cggagcaaga gtccgcatga gcctcgacac cctgccggag
     8161 ccgctgacgc agcttcatgc gcgactggcg gcgctggata tcgagcggga ggcgattgag
     8221 caggacagcg tgttttatcc cgaaaccagc ccggagcggc tggcagaact gaccgatttg
     8281 cgtgatgagc tgcaggcaga agccgggcat ctggaagcgc agtatcagca ggaaaaggca
     8341 ctggtgcagc agattatgac gttgcgtcag gaaggaacag acagctctga actgcaacag
     8401 caactgcgga cgcatcaggg ctttgcaccg ctgctggcgc tggatgtgga cgcccgcgcc
     8461 gtcgccacgg tggtggcgga ctggactggc atcccgctct cttccctgct caaggacgag
     8521 cagagcgacc tgctcagtat ggaacagagc cttgaaaacc gcgttgtcgg gcaacgcccg
     8581 gcgctctgcg ccatcgcaca gcggttgcgg gcggctaaaa ccggcctcac gccggagaac
     8641 ggcccacagg gggtattcct gctgaccggc cccagcggca ccggtaaaac cgaaactgcg
     8701 ctcacactgg ccgacaccct gtttggcggt gaaaaatctc ttatcaccat caacctctcc
     8761 gaatatcagg agccgcatac cgtctcccag cttaaggggt ctcctccggg ctacgttggc
     8821 tacggtcagg gcggcgtact taccgaagcc gttcgcaaac gcccgtacag cgtggtgttg
     8881 ctcgacgaag tggaaaaggc gcaccgcgac gtaatgaacc tgttctatca ggtatttgac
     8941 cggggtttta tgcgcgacgg cgaaggccgg gaaattgact tccgcaacac cgtgattctg
     9001 atgacggcta atctgggcag cgaccacatc atgcaactgc tggaggaaaa accggacgcc
     9061 acggacgcag acctgcatga actgctgtat cccctgctgc gcgagcactt ccagcctgca
     9121 ctgatggcgc gttttcagac ggtgatttac cgcccgctgg gacaggaggc gatgcgcacc
     9181 attgtggaaa tgaaactggc gcaggtggtt cgtcgtcttc atcagcacta tgggctggaa
     9241 acggaaatca acgacagcct gtatgacgcg ctgaccgccg cctgcctgct gccggacacc
     9301 ggtgcacgca atatcgacag cctgttgaac cagcaaatcc tgccggtact aagtcagcag
     9361 ttgctggcac agcaggccgc gcaccgtaaa ccagcccaac tacggcttgg ctgggatgag
     9421 gaagacggga ttgtactgga gttcgctacg gaagagatgc aataacggag gcttttatgg
     9481 gtattcagaa tttcgaccac tcacaccaca agctcaaaat tcgcggcctg aaaagcccgg
     9541 tggacgtgct gacgtttacg ggccgggaac aactcagcgc acccttccgc tacgacattg
     9601 aatttaccag taccgataaa accatcgagc cggagtcggt gctgatgcag gatggcgcat
     9661 tcagcctgac ggcaccgccg gtacagggaa tgccggtaca gactgccctg cgcaccctgc
     9721 acggcgtcat taccggcttt aaacatctct catcgtcaca ggacgaagcc cgttacgaag
     9781 tgcgccttga accccgtatg gcgctgctta cccgcagccg ccagaacgcc atctaccaga
     9841 accagacggt gccgcaaatc gtcgaaaaaa tcctgcgcga gcggcaccag atgcgcggtc
     9901 aggacttcgt gtttaacctc aaaagcgaat acccgtcccg tgaacaggtg atgcagtacg
     9961 gtgaggacga cctgacgttt atttcacgcc tgctgtcaga agtgggtatc tggttccgct
    10021 ttgccaccga cgcccggttg aaaatcgaag ttatcgaatt ttacgacgac cagagcggct
    10081 acgagcgcgg gctgacgctg ccgcttcgcc atccttctgg gctgtttgat ggtgaaacgg
    10141 aagccgtatg ggggctgaac accgcgtaca gcgtggtgga aaaaagtgtc tccacgcggg
    10201 actacaacta ccgtgaagcc acggcggaaa tgaccacagg ccagcatgac gccaccgggg
    10261 gcgataacac cacttacggc gaagcctacc actatgccga caacttcctg caacaaggcg
    10321 ataaagaggc agcggaaagc ggcgcgtttt atgcccgtat ccgtcacgaa cgctacctga
    10381 atgaacaggc cattctcaaa ggccagagca ccagttcact gctgatgccg gggctggaga
    10441 taaaagttca gggcgacgac gcgcctgcgg tgttccgcaa gggcgtgctg attaccggcg
    10501 tgacgaccag cgcggcgcgt gaccgcagct atgagctgac gttcacggcg ataccgtact
    10561 cagagcgcta cggctaccgt ccggcgctta tcccccgccc cgtaatggcc ggaacgctcc
    10621 cggcgcgggt caccagtacg gtgaagaacg acatctacgc ccatatcgac aaggacgggc
    10681 gctaccgggt caacctcgac tttgaccgcg atacgtggaa accgggctat gagagcctgt
    10741 gggtacgcca gtcgcgcccg tatgcgggag acacctacgg cctgcacctg ccgctgctgg
    10801 cgggaacgga agtcagtatc gcctttgagg aaggcaaccc ggaccgacct tatattgcag
    10861 gcgtaaaaca cgactcggca cacactgacc atgtgaccat tcagaactat aagcgtaacg
    10921 tcctcagaac cccggcgaac aacaaaatcc gcctcgacga tgagcgcggc aaagaacata
    10981 tcaaggtcag taccgagtac ggcggcaaga gccagctaaa cctcggtcat ctggtggatg
    11041 ccgggaaaca gcaacgcggc gaaggctttg aactgcgtac cgatatgtgg ggcgtggtgc
    11101 gggcaaagaa aggcatcttt atcagcgccg atgcgcagga taatgcgcag gggcaggtgc
    11161 gcgaaatggc tcctgcaatg gcgatactga atggcgcaca gtcacagatg aagtctctgt
    11221 ccactgatgc gcagacggcg aatgccgatc cggcagatct gtcatcgcag attgcgttgt
    11281 tgcaacagag cgtaaaagat ctgaaccagg ctgcgatcct gctaagcgca cccaagggcg
    11341 tggcgatcgc cagcggcgag cacctgcaac tggcggcggc aaagaatgtg atagccaacg
    11401 caggcaacaa cgccgatgtc ggcgcggtga aaaatatgtt tatcggcgtg ggacaggcgc
    11461 taagcgtgtt tgtgcgtaaa gcgggcatca agctgtttgc caacaagggg gcgatctcgg
    11521 tacaggcaca gaacgatctg atggaactgt tggcacagaa atcgatagag atcaccagta
    11581 cggaagatga gatcaagatc accgcgaaga agaaaatcac ccttaacggc ggtggcagct
    11641 acatccggct ggatgcctgt gggatagaag cgggaacgca gggagaatat aacgttaagg
    11701 ccggatatta cgggcgtaaa ccaaaggcga agctgacgcc ggagctgatg gcgtttcctg
    11761 taatatctgc gaaatttgat gagcaagtta gagtaattga tgatgacgga aaattcattg
    11821 ctaatgtacc ttactatata gaagac
//
LOCUS       DAZKGF010000150        11702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZKGF010000150 DAZKGF010000000
VERSION     DAZKGF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.150"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..593
                     /gene="ecpR"
                     /locus_tag="ACHOQV_003908"
                     /gene_synonym="matA"
     CDS             51..593
                     /gene="ecpR"
                     /locus_tag="ACHOQV_003908"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFP9632201.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            667..1254
                     /gene="ecpA"
                     /locus_tag="ACHOQV_003909"
                     /gene_synonym="matB"
     CDS             667..1254
                     /gene="ecpA"
                     /locus_tag="ACHOQV_003909"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFP9632202.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1311..1979
                     /gene="ecpB"
                     /locus_tag="ACHOQV_003910"
                     /gene_synonym="yagY"
     CDS             1311..1979
                     /gene="ecpB"
                     /locus_tag="ACHOQV_003910"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFP9632203.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2005..4530
                     /gene="ecpC"
                     /locus_tag="ACHOQV_003911"
                     /gene_synonym="yagX"
     CDS             2005..4530
                     /gene="ecpC"
                     /locus_tag="ACHOQV_003911"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFP9632204.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4520..6163
                     /gene="ecpD"
                     /locus_tag="ACHOQV_003912"
     CDS             4520..6163
                     /gene="ecpD"
                     /locus_tag="ACHOQV_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFP9632205.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6132..6842
                     /gene="ecpE"
                     /locus_tag="ACHOQV_003913"
     CDS             6132..6842
                     /gene="ecpE"
                     /locus_tag="ACHOQV_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFP9632206.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQTGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7155..7484
                     /gene="ykgJ"
                     /locus_tag="ACHOQV_003914"
     CDS             7155..7484
                     /gene="ykgJ"
                     /locus_tag="ACHOQV_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9632207.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7496..7662)
                     /locus_tag="ACHOQV_003915"
                     /pseudo
     CDS             complement(7496..7662)
                     /locus_tag="ACHOQV_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7733..8347)
                     /gene="yagU"
                     /locus_tag="ACHOQV_003916"
     CDS             complement(7733..8347)
                     /gene="yagU"
                     /locus_tag="ACHOQV_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFP9632208.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(8562..8747)
                     /locus_tag="ACHOQV_003917"
     CDS             complement(8562..8747)
                     /locus_tag="ACHOQV_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632209.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            8796..8953
                     /locus_tag="ACHOQV_003918"
                     /pseudo
     CDS             8796..8953
                     /locus_tag="ACHOQV_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835868.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(8968..9043)
                     /locus_tag="ACHOQV_003919"
     tRNA            complement(8968..9043)
                     /locus_tag="ACHOQV_003919"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9008..9010),aa:Thr,seq:cgt)
     gene            complement(9158..10411)
                     /gene="proA"
                     /locus_tag="ACHOQV_003920"
     CDS             complement(9158..10411)
                     /gene="proA"
                     /locus_tag="ACHOQV_003920"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP9632210.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCSLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAGALAQLQTGPAKVVAVKTEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     SRGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(10423..11526)
                     /gene="proB"
                     /locus_tag="ACHOQV_003921"
     CDS             complement(10423..11526)
                     /gene="proB"
                     /locus_tag="ACHOQV_003921"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP9632211.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
BASE COUNT         3005 a         3068 c         2925 g         2704 t
ORIGIN      
        1 tagtgacatg gcaaaatgat tacagcaggg actatgaggt taaaaaccat atggaatgtc
       61 aaaaccgttc tgataaatac atctggtctc cccatgacgc ctacttctat aaaggactat
      121 ctgaactgat tgtggatatc gacagattaa tttatctatc gctggagaaa atcagaaaag
      181 atttcgtgtt tatcaatctc aatacggatt ctttaactga gtttataaac cgtgataatg
      241 agtggttatc cgcggtaaag gggaaacagg tcgtattgat tgcggccaga aagtcagaag
      301 ccttagcaaa ttattggtat tacaacagca atattagggg cgtggtatac gctggactga
      361 gtcgtgatat tagaaaagaa ctggcctatg tgattaatgg caggttcctg agaaaagata
      421 ttaagaaaga taaaatcact gaccgggaaa tggaaattat ccgcatgacg gctcagggaa
      481 tgctgcctaa atcgattgcc agaattgaaa attgtagtgt gaagacagtg tatacccatc
      541 ggcggaatgc agaggccaag ctgtactcaa aattatataa gttggttcag taaactccag
      601 gcaagttagt tttaaaaatg actcaccggg acttcatgtc ctcaattcaa ctcgggaaga
      661 aaagcaatga aaaaaaaggt tctggcaata gctctggtaa cggtgtttac cggtacaggt
      721 gtagcgcagg ctgctgacgt aacagctcag gctgtagcga cctggtcagc aacagccaaa
      781 aaagacacca ccagtaagct ggttgtgacg ccactcggta gcctggcgtt ccagtatgcc
      841 gaaggcatta aaggttttaa ctcacaaaaa ggtctatttg acgtggctat cgagggtgac
      901 tcaacggcta ccgcctttaa actgacctca cgccttatca ccaacacctt aacccagttg
      961 gatacctcag gttccacact gaatgtgggc gtggattata acggcgcggc agtcgaaaaa
     1021 actggcgata ccgtgatgat cgataccgcc aacggcgtac tgggcggcaa ccttagccca
     1081 ctggctaacg gttacaatgc cagcaatcgt accaccgcac aggatggttt cactttctcc
     1141 atcatcagcg gcaccaccaa tggtaccacc gcagtaaccg attacagcac tctaccggaa
     1201 ggcatctgga gcggcgacgt tagcgtacag ttcgacgcta cctggaccag ttaatctctc
     1261 tgatgtacca gcaggggtac ccccctgctt tattcctgga tggaattgtt atgaaaaagc
     1321 accttctgcc tctcgctctg ctgttttccg gaatatcacc ggcccaggcg ctggatgtcg
     1381 gcgatatatc atcgtttatg aacagtgaca gcagcacgct aagcaaaacg atccaaaaca
     1441 gtaccgacag tggccgcctc atcaatatcc gtctcgaacg gctctcttca ccgcttgacg
     1501 acgggcaggt tatcgcaatg gacaagccgg atgagttgct actcactccc gccagcttgc
     1561 tgctacccgc ccaagccagc gaagtgatcc gcttcttcta taagggacca gcagatgaaa
     1621 aagagcgcta ctaccgcatt gtctggtttg atcaggccct cagtgatgcg cagcgcgata
     1681 atgccaaccg cagcgctgtg gccactgctt ccgcccgcat cggcaccatt ctggtcgtcg
     1741 caccccgcca ggcaaactac cactttcagt acgccaacgg ctccctgaca aatacaggaa
     1801 atgcgacgct gcggatcctc gcctacggcc cctgcctgaa agccgccaat ggtaaggagt
     1861 gtaaagagaa ttactacctg atgccgggca agtcgcgtcg ttttacccgc gtggacacgg
     1921 cggataacaa aggacgggtt gcactttggc agggtgataa gttcattccc gtgaaataga
     1981 tagctgtgca gatggataac gacaatgcct ttacgacggt tctccccagg actgaaagcc
     2041 cagtttgcct tcggcatggt ctttttgttc gttcagcccg atgccagcgc tgctgacata
     2101 agtgcgcagc aaataggtgg ggtgattatt ccgcaggcct tcagtcaggc gctccaggac
     2161 ggcatgagcg tcccgcttta tattcatctc gccggtagcc agggtcgcca ggacgatcag
     2221 cgaatcggca gcgcttttat ctggttggat gatggacagt tacgcatccg aaaaatacag
     2281 ctggaagaga gtgaagataa cgccagtgtc agcgaacaaa ctcgacagca gctgatgact
     2341 ctggccaacg ccccgttcaa tgaggccctt accatccccc tgactgacaa cgcgcagttg
     2401 gatctcagct tgcgccaact gctgctgcag ctggtggtca agcgcgaagc gctgggcacc
     2461 gtactacgct cacgtagcga agacatcggg cagtccagtg ttaacaccct cagcagtaat
     2521 ctgagctata acttcggcgt ctataacaac cagttgcgta acggcgggag caacacctcc
     2581 agctatctgt cgctgaataa cgttactgcg ctgcgcgaac atcacgtggt gctcgacggc
     2641 tcactgtacg ggattggtag cggtcaacag gacagtgaat tatataaagc gatgtatgaa
     2701 cgcgattttg ctggtcaccg atttgccggt ggaatgctcg acacctggaa cttgcagtcc
     2761 ttagggccga tgaccgccat ttcagcaggg aagatttacg gcctttcctg gggaaaccag
     2821 gccagctcca ccatcttcga cagcagccag tcggccacgc cagtgatcgc ctttttaccg
     2881 gcggcgggcg aagtacatct cacccgtgat gggcggctac taagcgttca gaacttcacc
     2941 atgggcaatc atgaagtgga tacccggggt ctaccgtacg gtatttacga tgtggaagtt
     3001 gaggtaatcg ttaacggtcg cgtgatcagc aaacgcaccc agcgggtcaa taagctgttt
     3061 agccggggac gtggcgtcgg tgcaccgctg gcgtggcaga tatggggcgg tagctttcat
     3121 atggatcgct ggtcggaaaa cgggaaaaag acgcgaccag ccaaagagag ttggctggca
     3181 ggtgcctcga cctccggctc actgagtacg tttagctggg cggcaacggg atatggatac
     3241 gataatcagg cggtgggtga aacccgtctg acgctgccgc ttgggggggc gatcaacgtt
     3301 aacctgcaaa acatgctggc cagtgacagc tcatggagca acatcgccag catcagcgcc
     3361 actctacctg gaggcttcag ttcgctgtgg gttaaccagg aaaaaacccg cattggcaat
     3421 caattgcgac gtagcgatgc cgacaaccgt gcaatcggcg gcacactcaa cctgaactca
     3481 ctgtggtcga agctgggtac gttcagcatc agctacaatg atgaccgccg ctacaacagc
     3541 cattattaca cggcagatta ctatcaaagt gtctacagcg gtacctttgg ttcgcttggc
     3601 ctgcgggccg gtattcagcg ctataacaac ggcgacagca gcgccaatac agggaaatat
     3661 atcgctctcg atctctcgct accactgggc aactggttta gcgcagggat gacccatcaa
     3721 aacggctaca ccatggcaaa cctgtcagca cgcaaacagt ttgatgaagg aaccattcgc
     3781 actgttggtg ccaatctgtc acgagccatc tccggcgata ccggtgatga caaaaccctc
     3841 agcggtgggg cgtatgcaca gttcgacgct cgttacgcca gcggaacgct gaacgtcaat
     3901 agcgcggcgg acggctacat caatactaat ttgaccgcca acggcagcgt cggctggcag
     3961 ggtaaaaaca tcgctgccag cgggcggacc gatggcaacg ctggggtgat attcgacact
     4021 ggcctggaga acgatggtca gatcagcgcc aaaatcaacg ggcggatttt cccgcttaac
     4081 ggcaagcgta actatctccc gctctctccc tatggaagat atgaggtgga gttgcagaac
     4141 agcaaaaact cactcgacag ttacgatatc gtcagcggtc gcaaaagtca tctgactctc
     4201 tatccaggca atgtcgctgt cattgagcca gaggtgaagc agatggttac cgtctccggt
     4261 cgtatccgtg cggaagacgg cacactgctg gctaacgcac ggattaacaa ccatatcggc
     4321 cgaacccgaa ccgatgaaaa cggcgagttt gtcatggacg tggataagaa ataccccact
     4381 atcgattttc gctacagtgg caataaaacc tgcgaagtgg cactggaact caaccaggcg
     4441 cgcggtgccg tctgggtcgg tgatgtggtc tgcagcggcc tctcatcgtg ggcggcggtg
     4501 acgcagacag gagaagagaa tgagagttaa cctactgata gcgatgataa tctttgcgct
     4561 aatctggcct gcaaccgcgc tcagagcggc agtgagcaaa acaacctggg cggatgctcc
     4621 ggcacgcgag tttgtgtttg tcgaaaacaa ctcagacgac aactttttcg tcactcctgg
     4681 cggggcgctg gatccgcgcc tgaccggtgc caaccgctgg accggtttaa aatacaatgg
     4741 ttcaggaacc atctatcagc aaagcctcgg ctacattgat aacggttaca acaccggcct
     4801 ttataccaac tggaagtttg atatgtggct ggaaaattca ccagtttcat ctcctttaac
     4861 tggcttgcgc tgcatcaact ggtacgcagg gtgtaatatg accaccagtc ttatcctgcc
     4921 gcaaaccacc gacgccagtg gattttatgg cgcgacggtg accagcggcg gcgcgaaatg
     4981 gatgcacggc atgttgtcag acgcgtttta ccagtatctg caacaaatgc ccgtcggcag
     5041 cagctttaca atgaccatca atgcctgcca gacctctgtg aactatgacg ccagcagcgg
     5101 cgcacgctgt aaggatcagg cctccggcaa ctggtatgtt cgcaacgtca cccatacgaa
     5161 agcagcaaat ctgcggttga taaataccca ctcgctggcg gaagtattta tcaacagcga
     5221 cggagtaccg actctgggcg aagggaacgc cgactgccgg acgcaaacca tcggcagccg
     5281 ttcaggatta agttgtaaga tggttaacta taccctgcaa acaaacggac tcagcaacac
     5341 ctcaatccat atattcccgg cgatcgccaa ctcgtcgtta gcctcggccg tcggggcgta
     5401 cgatatgcag ttcagtctga atggcagttc atggaaaccg gtgagcaata ctgcctatta
     5461 ctacaccttc aacgagatga agagcgcaga ctcgatctat gttttcttct cgagcaactt
     5521 ctttaaacag atggtgaacc tcgggatcag cgatatcaac accaaagatc tattcaactt
     5581 tcgctttcag aacaccacat caccggagtc tggctggtat gaattttcta cctccaacac
     5641 gctgattatc aaaccccgtg atttcagcat cagtattatc tccgatgaat atactcagac
     5701 accgtcgcgg gagggatatg ttggcagcgg cgagtcggca ctcgatttcg gctatatcgt
     5761 aaccaccagc ggtaaaacag ctgccgacga agtgctgatc aaggtgaccg gacccgcgca
     5821 ggtgattggc gggcgctcct attgtgtctt cagctccgat gacggtaagg cgaaagtacc
     5881 gttcccggcg acgctttcct ttattacccg caacggagct acaaaaacct acgatgccgg
     5941 gtgcgatgat agctggcggg atatgaccga tgcgctgtgg ttgaccacac cgtggactga
     6001 tatctctggc gaagtggggc agatggataa gaccacagtc aaattttcga ttccaatgga
     6061 taacgccatt tctctgcgta cggtagatga taacggctgg tttggcgaag tcagcgcttc
     6121 aggagagatt catgttcagg cgacgtggcg taacattaac taaggccctg ctgacagtgg
     6181 tctgtatgct ggcggcacct ttgacacagg cgatttcggt cggcaatctg actttttcac
     6241 tgccgtccga gactgacttt gtcagcaaac gtgtcgtgaa taacaacaaa agcgcgcgga
     6301 tataccgtat tgccatcagt gctattgata gcccgggcag cagtgaattg cgcacccgac
     6361 cggtggatgg tgaactactt ttcgcccccc gccagctggc gttgcagact ggtgagagcg
     6421 agtattttaa attttactat catggtccac gggataaccg cgagcgctac taccgggtct
     6481 catttcgcga ggtccccact cgtaacctga caaaacgcag cccaaccggc ggtgaggtca
     6541 gcacggagcc ggtggtggtg atggatacca ttctggtagt acgaccacgt caggttcagt
     6601 ttaaatggtc cttcgatcag gtgaccggaa cggtaagtaa caccggcaac acgtggttta
     6661 agctactgat taaaccagga tgtgattcga ccgaagagga aggcgatgcc tggtatctac
     6721 gtccgggaga cgtggttcat cagcctgagt tacgtcagcc ggggaatcat tatctggtct
     6781 ataacgacaa attcattaag attagcgatt cctgtccggc taagccccct tcggcggact
     6841 aagacttatc catcggacga gggaagaatt ccacctcaga gactcaaaaa tcgtttaaat
     6901 gatgtggaag cgatcgtcaa tgtcacctgt aacgatgagc tttctcatat gcacaccaca
     6961 gtgttcagga gacacggaaa ggtaccaggc ggcctgctaa cgagtggttt cttcactaat
     7021 taggacgtaa aatcggtaac ggctggaaat cattcaatac tcacactatc gaaagtacac
     7081 cagccaaccg cgatacatcc ctgcatacga cgtaccgcag ttctctttac gatttatagc
     7141 tgtactggtg aattatgagc aatctgaatc catgcatgac gtgtggtgcc tgttgtgcat
     7201 ttttccgcgt ctctttttac tgggccgaag ctgacgatgc tggcggtacc attcccgcca
     7261 ggctcactga acaaatatcc ccttttcacc gatgcatgag cggtactaat cagaaaaatc
     7321 cccgatgtat tgcccttgca ggaacaccgg gcaaaaatgc ctgctgcacg atatatgaaa
     7381 atcgatcgtc cacatgcaga gaattcgcca tgtctggtga gaacggagaa gtcaatgagg
     7441 cttgcaatcg tgcaagagct aaatacgggc taacaccgtt ataaatatac aataattaat
     7501 tgcattgccc cgccagctgt aatagcgggg cttcgttttt cagggacaac aaaacctgaa
     7561 acttcttcca ttttccagca acaatgcctc ttcttctgta gcgttctccg gatctacatc
     7621 atcctgaagt agcgcgatat gatcactgac tgtccgcatc agaatatgaa aagcaacgtt
     7681 tttaacctat aaaaatggcg ctatatttgc gccattttta tcattcaaag cattatctat
     7741 ttgagcctaa agggatctca ggatctggct catgggtaat tctgtttcgt aaatctctgc
     7801 gaataatttc aatagaccag aaccagacta aatgtccaaa aatttcagag acattctcat
     7861 accacggaag atcaaagaga ggtggcgtaa gtcccatgag agggaatgaa atcatatgaa
     7921 caaaaagttg cgctaaagca cctgccagta agccctgcca aagtttaatt ttcggaaata
     7981 cttcggcgac cacacagtaa ccaacggcaa aaactatcga aaagattatg tgcgttacac
     8041 caacccagtt aaagacatgc ccggcgaagg tataaacagc cgaatttgga tctgtcagtc
     8101 ccaaccaatc ccgaagaaaa atgtacggag gattgagaaa gttacgggag caatcaattt
     8161 ggcctgcagc cctgattaat gattccggac cacacgctgc attaaacata tcaaccggac
     8221 tacgtggtgg caatggaact tctgcccccc acttaacaaa tgcggaaaca acgccagcaa
     8281 tcagcccaat gaatgcagca agaccataac gtctgcggtt tggtggagtt tgttcaaata
     8341 tattcatatc taccctgctt gtaccattat gttatacacc tctttaggag tattcataaa
     8401 acaaggcaaa tgtaaagaac tgtattgttt tgtataacca catggttacc taatcgccaa
     8461 ttaatataag ctccattatt tctccatatt ttcatgttag aagtgacaag gagaaatgta
     8521 atgtttctct cagagaatat aaatcattcc ttttgcccac cttaatggtg ggctttttta
     8581 tgcaaaacct gaacgtcctg tggtattcct ctggcataat gattttcgcg attaaccaac
     8641 aaccagacca gcaaagacgc caccacaagc acagtaacat ccatcaggct tgccacaccc
     8701 catgcgtgcg gctcaaagcc gccatggcat attaatccgc ttgccatttt cgaacagctg
     8761 attggtttgg tccggtgttt ccctgcttgc acttaaaaat gagcggatgg gaaactctcg
     8821 aaatggttcg tcgatatgct cacctcgcac ctaatcacct taccgaacac gcacggtaaa
     8881 tagattcgat cctgaaccca tcggtcccaa atctgtccca ataaaaaata aggaaggtat
     8941 tagtgatgtg taacttattg atttaaatgg tgccgataag aggagtcgaa cctccgacct
     9001 tcgcattacg aatgcgctgc tctaccaact gagctatatc ggccctgaaa ggacatgttc
     9061 acgaacgtga atcacggtgg acaaggttaa aactaaccgg gcgatgcgtc aatggccttg
     9121 tgaatcaaat ggctactttt gcatcacccg gttttattta cgcacgaatg gtgtaatcac
     9181 caatgccgat ccacttgtaa gtggtcagtg cttccagccc cattgggcca cgcgagtgga
     9241 gtttttgtgt gcttaccgcc acttccgcac ccagtccaaa ctggccgccg tcggtaaaac
     9301 gcgtagaggc gttaacgtaa acagcggagg aatctacttc gttaacaaaa cgctgggcgt
     9361 tgcgcatatc gcgggtcagg atcgcatcgg agtgttgcgt gccgtgttca cgaatatggg
     9421 cgatggcatc gtcaagatcg ctgacgattt tgacgttcaa atctaatgac agaaactcat
     9481 cgtcatactc ttcggtttta acagcaacca ccttcgcggg gcctgtctgc aactgcgcca
     9541 gtgcacctgc atcagcgtgt aacgtcacgc cactttccgc catttgtttg cttaatgcgg
     9601 gcaggaaact atcggcgatg tttttattca ccagcaacgt ttcaaccgta ttacatgtgc
     9661 tcggtcgctg agttttcgcg ttgacgatca ctttcagggc ttcagcgatc tctgcacttt
     9721 catcaacgta aatatggcat acgcctatac cacctgtgat caccgggatc gtcgactgtt
     9781 cgcggcacag tttatgcagg ccagcgccac cgcgcgggat cagcatgtcg atgtatttat
     9841 ccatacgcag catttcactg accagcgcgc ggtcaggatt atcaatcgcc tgcacggcac
     9901 ccgccggtaa gctgcaggat ttcagggcgt cctgaatcac cgccaccgtt gcagcgttag
     9961 tgcgacacgt ttctttgccg ccgcgcagga tcaccgcgtt acctgttttc aggcacagcg
    10021 aagcgacatc taccgtcacg ttcgggcgcg cttcataaat cacgccaatc acccccagcg
    10081 gcacgcgacg gcgctccaga cgcagaccac tgtccagtac gccgccatcg attacctgcc
    10141 ccaccggatc ggcgagatta cacacctgac gcacatcgtc ggcgatgcct ttcagccgtg
    10201 cgggcgtcag tgccagacgg tcaagcatcg cttcgctaag gccattggca cgcgcgtcag
    10261 caacatcctg ggcgttagcg ttgaggatga tttcgctttg tgcttccagt tcatcggcga
    10321 ttttttccag cacacgattt ttttcgcggc tggagagttg cgctaattta tacgaggctt
    10381 gctttgcggc aatgcccatt tgttccagca tcggccagct ccttaacggg taatcatgtc
    10441 atcacggtga acggcaaccg ggccatattc atatcccagt attgcatcaa tttcttgcga
    10501 gtggtgtccg gcaatgcggc gtaatgcatc gctgttgtaa cgactgacgc cgtgggcgat
    10561 atcgcgacct tcgaggttgc aaatgcggat gacttcacca cgcgagaaat tgccagttac
    10621 gctcttaatg cctttcggca acagggagct gccgcgttca agaatggcgg cggttgcccc
    10681 ttcatctacc gtgatttcac ccgccggcgg cgcaccgaaa atccagcgtt tacggttttc
    10741 aagcggagtc gcctgggcat ggaacagcgt accgacggaa atgccttcca tcacatcacc
    10801 aataacgccc ggcttgctgc ccgcggcaat aatggtgtcg atacccgcac ggcaagccac
    10861 gtccgcggcc tgcaatttgg tgctcatacc gccagttccg aggcctgaaa cgctatcacc
    10921 ggcaatcgcg cgcagtgcgt catcaatgcc gtacacatct ttaatcagtt ctgcctgcgg
    10981 attgctgcgc gggtcagcgg tatacaaacc tttttgatcg gtcagcagca acagtttatc
    11041 ggcacccgcc agaatcgccg ccagcgcaga aaggttatcg ttatcaccga ccttaatctc
    11101 tgccgtagcg acagcatcgt tctcattaat taccggaacg atattgttat cgagcaacgc
    11161 acgcagggta tcgcgggcgt tcaggaagcg ttcacgatct tccatatcag cacgggtcag
    11221 caacatctgc ccgacatgaa tgccgtaaat tgaaaacagc tgttcccaca gttgaatcag
    11281 tcgactctgc cctaccgccg ccagcagctg ttttgaagcg atagtcgctg gcaattccgg
    11341 gtaccccagg tgctcacgtc cggcggcaat cgcacccgac gtcacaataa caatccgatg
    11401 cccggcggca tgtaactgcg cgcactggcg aacaagttca acgatatggg cacggttcag
    11461 gcggcgcgat ccgcctgtta gcacactggt gccgagtttt accaccagcg tctggctgtc
    11521 actcatgatt ctctgccatt caattttagg aaaaaatgat atcaaacgaa cgttttagca
    11581 ggactgtcgt cggttgccaa ccatctgcga gcaaagcatg gcgttttgtt gcgcgggatc
    11641 agcaagcgta gcggcagttg tttacgcttt tattacagat ttaataaatt accacatttt
    11701 aa
//
LOCUS       DAZKGF010000151        11581 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZKGF010000151 DAZKGF010000000
VERSION     DAZKGF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.151"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..455)
                     /locus_tag="ACHOQV_003922"
     CDS             complement(<1..455)
                     /locus_tag="ACHOQV_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632212.1"
                     /translation="MYNNLYALTESLFWVDRLLEQEYENIGLNELLVELAEYNKRIDS
                     FIIEQTNSVQESRLNVYMAGNRNIERYKSGLFSMDRVILDDVLYSLSLRYRVNLSENV
                     VEKFIESDSKEKVEHLRKEIARFIRFAKENFDLIRGEFIVFVRRESSIK"
     gene            1468..1644
                     /locus_tag="ACHOQV_003923"
     CDS             1468..1644
                     /locus_tag="ACHOQV_003923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632213.1"
                     /translation="MNMNEDEIYRHIRQALSSAPRNQYTVELHLQMIKYADELEHITA
                     KAFCEGTGLNTDLL"
     gene            1684..4122
                     /locus_tag="ACHOQV_003924"
     CDS             1684..4122
                     /locus_tag="ACHOQV_003924"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFP9632214.1"
                     /translation="MAIKKTELYSSLWASCDELRGGMDASQYKDYVLTLLFMKYVSDK
                     YKGDPYGMIVIPKGASFDDMVALKNDKEIGDKINKIIHKLAEENGLKGVIDEADFNDE
                     DKLGKGKEMIDRLSKLVGIFEGLDLSSNRVEGDDLLGDAYEYLMRHFATESGKSKGQF
                     YTPAEVSRILAKVIGITPDTPRDATVYDPTCGSGSLLLKVNDEARRGLSIFGQEMDNA
                     TSALARMNMILHNNATAKIWQGNTLSDPQWKEANGKLKAFDFAVANPPFSNKNWTNGL
                     TPKKDPFERFGWGIPPEKNGDYAFLLHIIKSLKSTGKGAVILPHGVLFRGNAEANIRE
                     NLIKQGYIKGVIGLPANLFYGTGIPACIIVIDKEHAHSRKGIFMIDASRGFIKDGNKN
                     RLRSQDIHRIVDVFNHQRTVPGYSRMVPSSEIAGNDYNLNIPRYIESGEPEDLHDLTA
                     HLQGGIPVRDVDALKDYWRVFPALRNVLFADDHPGYCRAQVDAQQVKPTILAHQEFKD
                     FATRSLLPFKSWVKEAGLEEIRKGDKPKALIHDISEMLLAQYANSELLNKYSVYQILM
                     DYWADVMQDDVYVIMQDGWKAAAQIRELQPVKGKDGKNIWKETHDFEFTKRRYKADVL
                     PRSLVEIRCFPELLDALNRAQERSEEASRQLVEFIEEQAGEEDLLAEVKNDKDKVTQK
                     LVNARLAQLKPPPADPDELAVLNRCLALIKAEASAKKALKAAQEALDRAVFKHYPTLD
                     EAAIKTLVVQDKWLATLQAGIKAEIERITQQLASRVKELEERYAEPLPALEAAVEALS
                     EKVAGHLRAMGLEW"
     gene            4122..5471
                     /locus_tag="ACHOQV_003925"
     CDS             4122..5471
                     /locus_tag="ACHOQV_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9632215.1"
                     /translation="MVDLMVNEQRSGTAGKDVPAGYKLTEVGVIPEDWVVISIDDCTT
                     KVGSGKTPTGGASVYIDSGRPFVRSQNVDWGRLDLADVVHITDETHNTFSGSEIQQDD
                     VLLNITGASIGRCAKADFRVIGGNVNQHVCIIRTEAKKLSPSLLVELINSNYGQNQID
                     SYQAGGNREGLNFSQVKNLKFAITPNSFEQTEIVRTLNDINAFTLALENLIAKKQAIK
                     AAAMQQLLTGKTRLPQFALRKDGSAKGYKKSELGEIPEDWNCINLGELGNCIIGLTYK
                     PEDTSDYGTLVLRSSNIQNNHLAYDDNVFVSIEPPERTIVKEGDILVCVRNGSRQLIG
                     KCALIDKNAQGSAFGAFMSVFRTDIYGFTFHQFQSDIIQKQIAEVMGATINQITNKDM
                     LGFKIPLPENTEEQAAIAAILSDMDKEIQILQQRLDKTRQLKQGMMQELLTGKIRLI"
     gene            5484..6140
                     /gene="darT"
                     /locus_tag="ACHOQV_003926"
     CDS             5484..6140
                     /gene="darT"
                     /locus_tag="ACHOQV_003926"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system toxin DNA ADP-ribosyl
                     transferase DarT"
                     /protein_id="HFP9632216.1"
                     /translation="MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAEN
                     WINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTPFSPMLMNIHSGRGGIKQRPNEE
                     IVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
                     PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS
                     "
     gene            6137..7204
                     /gene="darG"
                     /locus_tag="ACHOQV_003927"
     CDS             6137..7204
                     /gene="darG"
                     /locus_tag="ACHOQV_003927"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin DNA ADP-ribosyl
                     glycohydrolase DarG"
                     /protein_id="HFP9632217.1"
                     /translation="MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMK
                     VYALACKQKQVITGKMFITETGELMGPRWIVNFPTKQHWRADSRMEWIEDGLQDLRRF
                     LIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
                     GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAH
                     YYGPYAPNLNHLLNALDGTYLKAEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWL
                     PALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEGLHQWPAGERWASRK
                     LRLFDNNNLQFAINRVMEFHC"
     gene            7223..10318
                     /locus_tag="ACHOQV_003928"
     CDS             7223..10318
                     /locus_tag="ACHOQV_003928"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015872650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFP9632218.1"
                     /translation="MSTVGQRERATQQRLIQFFVEDLGYRHLSHRTGYTGNSNIDTNI
                     LSAWLKRRGVNDTLITRALRQLDSAAALGEGKKLYYANKEVYRLLRYGVKDKEGAGEQ
                     NQTVWLIDWANPQANDFAIADEVTVKGEYCKRPDLVLYVNGIALGVIELKRSSVHISE
                     GIRQNLDNQKKTFIRDFFTTMQLVMAGNDTQGLRYGTIETPEKYYLEWKEDNPGDYTH
                     KLDFHLSRLCSKSRLLDIVHNYIAFDVGVKKLCRHNQFFGVEAAKQHIARREGGIIWH
                     TQGSGKSLTMVWLAKWIRENVPNSRVLIVTDRTELDEQIESVFMGVNEDIYRTSSGND
                     LIATLNHPNPWLICSLVHKFGRRSEAEDNAATDAFITELQQSLTKTFRAKGDLFVFVD
                     ECHRTQSGKLHNAMTAILPDALFIGFTGTPLMKKDKKKSVEVFGPYIHTYKFDEAVAD
                     GVVLDLRYEARDIDQYLTSEKKVDDWFEAKTRGLSNLARTQLKQKWGSMQKLLSSKSR
                     LEQIVNDILLDMDTRPRLMDGRGNAMLVCSSIYQACKVYEMFSQTELAGKVAIVTSYR
                     PDAASIKGEETGEGLTEKLSKHATYRKMLADYFEQSEEKAAARITEFEREVKQRFIEE
                     PGQMRLLIVVDKLLTGFDAPSATYLYIDKTMTDHTLFQAICRVNRLDGEDKEYGYIID
                     YKDLFRSLDKAITDYTTGAFDDYDRDDVAGLLKDRLEQAQLDLDSALEMVRSLCEPVK
                     TPRSLQDYQHYFCGESGENQTSLNEKEALRLTFYQCVARLLRAYASIANEMPEAGYTA
                     EEVESIRAEVAEFDKLRYEIKLTSGDLLDMKRFEPAMRHLLDMYVRADDSEVLMDFEE
                     LSLIELIVEKGLAATVALPDDIRNNQDAMAETIENNVRKTIVDENPVNPKYYGRMSQL
                     LDELITLRRQNALNYQQYLERIRDLCKQVIRPEQTAGACYPATMDTQAKRAFYDNFGH
                     DEVLATRIDTTIRYTKRAEWIGDRFKEREIANALREETASYNIDIDEVIALARQQKEY
                     H"
     gene            10318..11034
                     /locus_tag="ACHOQV_003929"
     CDS             10318..11034
                     /locus_tag="ACHOQV_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP9632219.1"
                     /translation="MPIMQIGSLHLLVNRKAIKNLHISVLPPDGKVRVSAPEHMTETA
                     IRMAVASRFRWIRKQQQDFARQPRQSEREMVSGECHYLWGRRYRLLVIERHGRHEVKV
                     AGNNKLQLYVQPGTRDENKALALSEFYRHELKQQVAKLLPEWQDRIGVPLTFWGIKKM
                     KTFWGTCNTTTKRIWLNLELIKKPPECLEYILVHELVHLLERHHNARFRLHMDRFIPD
                     WRERRDLLNNMPLAFEAWEY"
BASE COUNT         3205 a         2699 c         2959 g         2718 t
ORIGIN      
        1 tctttaatag aggattctct tcgtacaaaa acgataaact ctccacggat aagatcaaag
       61 ttttctttag caaacctgat aaacctagca atttccttcc tgagatgttc aaccttctct
      121 ttgctatcgc tttcaataaa tttttcaact acgttttctg atagatttac cctgtatcgt
      181 aggctcaatg aatatagaac gtcatcaaga atgactctgt ccattgaaaa caaaccactc
      241 ttataccttt caatattacg attccccgcc atgtaaacat tcagccggga ctcctgtaca
      301 gaatttgttt gttcgataat gaatgaatct atccttttat tatattcagc taattcaact
      361 agcaattcgt tcaatccgat attttcatat tcctgttcta aaagtcgatc aacccaaaaa
      421 aggctttctg tgagcgcata gaggttgttg tacatactag ctccttttta ccaagcttca
      481 gtactgtgtt ctcaagatta ctcagtggtt ttcacacaga gtgcactctc attttacatt
      541 taaatgtact tcctagcatc ctgctgttag tgccggatta cagcacttat caactgtata
      601 tttagaaaac aaactgcgca taatcctagt gagttgttat gtccgctcct cgctcaaagc
      661 agaccattcg ctcagttaac gcatcagtgt cgtgctaaaa gtagacgtgg ttaacaacaa
      721 tgtgagttaa tcaacggaga gcaggtcaac tctaacctgt atcaaaaaaa caaatgtgtc
      781 acaaagatgc cgcatgcctt aatttgagta taattgagac ggaagtagac ggtttatggc
      841 taaatattgt tgatatatag ctatttgaag agttgtagcg caagtgtata gcccaaacta
      901 ataacgctac cccttacctt tgacgcctct tatcaaagag ctgcgatgat gcacagcccg
      961 cacgaggcaa tattcgcaca agaatcaggt tcagaaataa cctgctctgg catggtgggg
     1021 agagtatcac tgatcaggct ttcgttaaga tgcggcggga tgaggccgtg ttgcgtgagt
     1081 tgcagcagta caagatcgcc cgcagagggt aagctacagc gcgagatcct aggtttctat
     1141 cagggattcg gcaaagcggg cgcagttgtc gaccaaggcg gtgaaatcta tagtggcatt
     1201 ataagggaac gaacaataga tagagtaaat cgcagtcagg gacaaggatt gtagtctata
     1261 gagaagtctc ctgtaacggt tttcaggagg ctaattccga cgcctgattt tgcaggcgcg
     1321 gagggagagg atactgcgac cattaatccg ccacaaagca tttacgtgca taaatcacgc
     1381 agtcagatcc gactctcact gtagttcttg aacctgatac cacatttagc taatttagct
     1441 aacttatcca gtataccagt ggttgccatg aatatgaatg aagacgagat ttatcgtcat
     1501 atccggcagg cactttccag tgcgccgcgt aaccagtaca ccgtcgagct gcacctgcaa
     1561 atgattaaat atgccgacga actggaacac attacggcaa aagccttttg cgaaggcacc
     1621 ggtctgaata ccgatctgct gtaaccgact tacggcacat tatcgcgcaa aacgagatat
     1681 ttaatggcta tcaaaaaaac cgaactctat tcttccctgt gggcaagctg cgacgaactg
     1741 cgtggcggga tggacgccag ccagtacaaa gactatgtac tgaccctgct gtttatgaag
     1801 tacgtttccg ataagtataa aggcgatcct tacgggatga tcgtgatacc caaaggagcc
     1861 agctttgacg atatggttgc gctgaaaaat gacaaagaga tcggcgataa aatcaacaaa
     1921 atcatccaca agctggcgga agagaacggc ctgaagggcg tcatcgacga agccgacttt
     1981 aacgacgaag ataaactcgg caaaggcaag gaaatgattg accgcctgag caagcttgtc
     2041 ggtatctttg aagggttaga tctctccagc aaccgggtcg aaggcgacga tctgctgggc
     2101 gatgcctatg aatatctgat gcgtcacttc gcaaccgaat ccggcaagag taaaggacag
     2161 ttttacactc cggcggaggt gtcacgtatt ctggcaaagg tgattggtat tactcccgat
     2221 acgccgcgag atgcaacggt ttatgaccca acctgtggct ctggttcgct gctgttgaaa
     2281 gtgaatgatg aagcccggcg cggactgtcg attttcggcc aagagatgga taacgctacc
     2341 agtgcgctgg cgcggatgaa catgattctg cataacaatg ccaccgcgaa aatctggcag
     2401 ggcaatacgc tgagtgatcc gcagtggaaa gaggcgaacg gcaaactgaa agccttcgat
     2461 tttgccgtgg cgaacccgcc tttttccaat aaaaactgga ccaacgggct gacgccgaaa
     2521 aaagatccgt ttgaacgctt cggctggggt attccgccgg aaaaaaacgg cgattatgct
     2581 tttttgttgc atatcattaa aagcctgaaa agtaccggca aaggcgcggt gatcctgccc
     2641 cacggcgtac tgtttcgcgg taacgccgaa gcgaatatcc gtgaaaacct aatcaagcag
     2701 ggctatatca aaggcgttat tggtttgcca gccaacctgt tttacggcac cggcatcccg
     2761 gcgtgcatca ttgtgattga taaagagcac gcccacagcc gcaaaggcat ttttatgatc
     2821 gacgccagtc ggggctttat caaagatggc aacaagaacc gcctgcgtag ccaagatatt
     2881 caccgtattg tggacgtgtt caaccatcag cgaacggtgc caggctacag ccggatggtg
     2941 ccctccagcg agatcgccgg gaatgattac aacctgaata ttcctcgtta tatcgagagt
     3001 ggagagccgg aagatttgca cgatctgacc gcgcacttac aaggcggtat tccagtgcgc
     3061 gatgtagatg ctttgaagga ttactggcgg gtattccctg ccctgcgtaa tgtgctgttt
     3121 gctgacgacc accccggtta ctgccgggcg caagtggacg cgcagcaggt gaaaccgacc
     3181 attctggctc atcaggagtt taaggatttt gccacccgca gcctgctacc gtttaagtcg
     3241 tgggtaaaag aggcaggtct ggaagagatc cgcaaaggag ataaacccaa agcgctaatc
     3301 cacgatatca gcgaaatgct attggcgcag tacgccaaca gtgagttgct gaacaaatac
     3361 agcgtgtacc agatcctgat ggattactgg gctgatgtca tgcaggacga tgtgtatgtg
     3421 attatgcagg atggctggaa agccgccgcg caaatccgcg agcttcagcc ggtgaaaggc
     3481 aaagatggca aaaacatctg gaaagaaacg cacgatttcg agtttaccaa acggcgctac
     3541 aaggccgatg tgctgccccg ttcgctggtg gaaattcgct gtttcccgga actgctggat
     3601 gcgctgaacc gcgcgcagga gcgcagtgaa gaagccagtc gtcagctagt ggaattcatc
     3661 gaggagcagg caggggaaga ggatctgctg gctgaggtga aaaacgataa agataaggtg
     3721 acgcaaaagc tggtgaatgc ccgtctggca cagttgaagc ccccccctgc cgacccggat
     3781 gagctggcag tgctgaaccg ctgtctggcg ctgattaagg ccgaggccag tgcgaaaaaa
     3841 gcgctgaaag ccgcacagga agcgttggat cgagcggtat ttaaacatta tcccacgctg
     3901 gatgaagcag cgattaaaac gctggtagtg caggataagt ggctggcaac gttgcaggca
     3961 gggatcaaag cggagataga gcgtattacc cagcaactgg cgtcgcgcgt aaaggagctg
     4021 gaagagcgtt atgctgaacc gttgccagca cttgaagccg ccgttgaggc gttgagcgag
     4081 aaagtggccg ggcatttgcg ggcgatgggg ctggagtggt gatggtggat ttaatggtga
     4141 atgagcaacg gtcgggtacg gcggggaaag atgtgcctgc ggggtataag ctgactgagg
     4201 tgggggtgat tcctgaggat tgggtagtaa tctctattga tgactgtaca accaaagttg
     4261 gtagtggaaa aacaccaact ggtggagcat ctgtttatat tgatagcgga cgcccttttg
     4321 tccgtagcca aaatgttgat tggggaagat tagatctcgc cgatgtcgta catattacag
     4381 atgaaaccca taacacattc tctggttctg aaattcagca agatgatgtt ttactcaaca
     4441 taacgggagc atctatcggc cgatgcgcta aagctgattt tagggttata ggtggtaatg
     4501 ttaaccaaca tgtttgcatc atcagaacag aggcaaaaaa actctcccca tccttactgg
     4561 ttgaattaat caattcaaat tatggacaaa accagattga tagttatcaa gccggaggaa
     4621 acagagaagg actgaatttt tctcaagtta aaaatcttaa gtttgctatt acaccaaact
     4681 cctttgaaca aacggaaatt gtcagaacat taaatgatat taatgcattt actctcgctc
     4741 tggaaaactt aattgctaaa aaacaggcca taaaagccgc tgctatgcaa caactactta
     4801 ccggtaaaac ccgtttgcca cagtttgcgt tgcgcaaaga tggctctgct aagggatata
     4861 aaaagagcga gttgggggag attcctgaag attggaattg tattaatctt ggtgagctag
     4921 gtaattgcat tattgggtta acttacaaac ctgaagatac atctgattat ggtacattgg
     4981 tattacgctc atccaacatt caaaataatc acttagctta tgatgataac gtcttcgtca
     5041 gcatagaacc acctgagcgc acaattgtga aagaaggtga tatcttggta tgtgtaagaa
     5101 atggcagtcg ccaacttatt ggcaaatgcg cattaattga caaaaatgcg cagggctcag
     5161 cattcggtgc atttatgtca gtattccgaa ctgatatata tggatttaca tttcaccaat
     5221 tccagtctga tattattcaa aagcaaatcg ctgaagttat gggtgccact atcaatcaga
     5281 taactaacaa agatatgttg ggctttaaaa tcccattacc tgaaaataca gaagaacaag
     5341 ccgccatcgc cgccatcctc tccgatatgg acaaggaaat ccagatcctg caacagcgtc
     5401 tggacaaaac ccgccagctt aagcaaggga tgatgcagga actgttaacc gggaaaatcc
     5461 gcttaatata gaagggaaag cacatggctt atgattacag cgccagcctc aacccgcaaa
     5521 aagctctgat ctggcgtatc gtccatcggg acaatatccc gtggattctg gataacgggc
     5581 tgcactgcgg caacagtctg gtgcaggcgg aaaactggat caatatcggc aacccggagc
     5641 tgatcggcaa gcgtgccggg catccggtac ctgtcggaac gggcgggaca ctgcatgact
     5701 atgtgccgtt ttattttacc cccttctcgc caatgctgat gaacatccac agcgggcgcg
     5761 gcggcattaa gcaacgcccc aatgaggaga tcgttattct ggtcagcaac ttgcgaaacg
     5821 tggcagcaca cgacgtaccc ttcgtgttta ccgacagcca tgcttactat aactggacca
     5881 actactacac cagtctggat agtctggacc agatcgactg gcccattttg caggcacggg
     5941 actttcgccg cgatccagac gatccggcta aatttgaacg ctatcaggca gaggcgctaa
     6001 tctggcaaca ctgcccgatc tctctgctgg atgggataat ttgttacagc gaagaggtca
     6061 ggttacaatt agaacaatgg ctgtttcagc gtaatctaac aatgtccgtt cacacacggg
     6121 ctggatggta cttctcatga tcacatatac acaaggcaat ttactggacg caccggtgga
     6181 agctctggtg aatacggtta ataccgtcgg tgtcatgggg aaaggcatcg cgttgatgtt
     6241 taaagagcgc ttccccgaaa atatgaaagt ctatgccctc gcctgcaaac aaaagcaggt
     6301 gatcaccggc aaaatgttca ttaccgaaac cggggaactg atgggaccgc gctggattgt
     6361 taattttccg accaaacagc actggcgcgc tgattcccgc atggagtgga ttgaagatgg
     6421 cttgcaggac ttacgccgct ttttgattga agagaacgtg cagtccatcg ccattccacc
     6481 actgggcgca ggcaacggtg gtttgaactg gccggacgtt cgggcgcaga ttgaatctgc
     6541 ccttggcgac cttcaggacg ttgatatcct gatttaccaa ccgactgaaa aataccagaa
     6601 cgttgccaaa agcactggcg taaaaaagct cactcccgcc agagccgcca tcgctgaact
     6661 ggtgcgtcgc tactgggtat tggggatgga gtgcagccta ctggaaatcc agaaactggc
     6721 ctggctgtta caacgcgcca ttgagcagca tcaacaggac gatatcctca aactgcggtt
     6781 tgaagctcac tattatggcc cctatgcgcc caatctgaac catctgctta atgcgctgga
     6841 cggcacctat cttaaagccg agaaacgcat cccggacagc caaccgctgg atgtgatctg
     6901 gtttaacgat cagaaaaaag aacacgttaa tgcgtatctg aacaacgaag ccagagagtg
     6961 gctacctgcg ctggagcagg tcagccagtt aattgacggc tttgaatcgc cgtttggtct
     7021 ggagttgctg gcaaccgtag actggttgct gagccgtggc gaatgtcaac caacgctgga
     7081 ttccgttaag gaagggctgc accagtggcc tgcgggcgaa cgctgggcca gccgtaagtt
     7141 gaggctgttt gataataata atctgcaatt tgccattaac cgcgtaatgg agttccattg
     7201 ctgagggttt aaggagcgcc ttttgagtac cgtgggccag cgcgaacgcg ccacacagca
     7261 gcgtctgatt cagttttttg ttgaagattt aggttaccgc catctgagtc accgcaccgg
     7321 gtacaccggg aacagcaaca tcgacactaa tatactgagc gcgtggctaa aacggcgcgg
     7381 cgttaatgat acgttgatca cccgggcgtt gcggcaactg gattccgccg ccgctctggg
     7441 cgaaggcaaa aaactctatt acgccaataa agaggtctat cgcctactgc gttacggcgt
     7501 gaaagacaaa gaaggcgcag gcgagcagaa ccaaaccgtg tggctgattg actgggccaa
     7561 cccacaagcc aatgatttcg ccatcgccga tgaggtgacg gtcaaaggcg agtactgcaa
     7621 gcgcccggat ctggtgctgt acgttaacgg cattgcgctg ggcgtgattg aacttaagcg
     7681 ttcgtcagtg cacatcagcg aaggcatccg gcagaatctc gataatcaaa agaaaacctt
     7741 tatccgcgat ttcttcacca ctatgcagtt agtgatggcg ggcaacgata cgcagggcct
     7801 gcgctacggc accatcgaaa ccccggaaaa atactatctg gagtggaaag aagataatcc
     7861 aggcgactat acacataaac tcgactttca tttaagtcgt ctgtgtagca aatccaggct
     7921 gttggatatc gtccataatt acattgcctt tgatgtcggg gtgaaaaaac tctgccgcca
     7981 taatcagttc ttcggcgtcg aggcggctaa acaacatatt gcccgtcgtg aaggcggtat
     8041 tatctggcac acacagggat cgggtaaaag cctgacgatg gtctggctgg cgaagtggat
     8101 ccgtgaaaat gtgccaaact cccgcgtgtt gattgtgact gaccgcaccg aactggatga
     8161 acagatcgaa agcgtgttta tgggcgtgaa tgaagatatt tatcgcacct ccagcggcaa
     8221 cgatcttatc gcaacgctca atcatcctaa cccgtggctc atttgctcgc tggtgcataa
     8281 atttggtcgt cgcagcgaag ctgaagacaa tgcggctacc gacgccttta tcaccgaatt
     8341 acagcagtcc ctgacgaaaa cattccgcgc caaaggcgat ctgtttgttt ttgtggacga
     8401 gtgccaccgc acacaatccg gcaagctgca taacgccatg actgccattc tgccggacgc
     8461 cctgtttatc ggttttaccg gcacaccgct gatgaagaaa gataagaaga agtcggttga
     8521 ggtgttcggc ccttatatcc atacctataa atttgatgaa gcggtggccg acggtgtggt
     8581 gctggatctg cgctacgagg cacgcgatat tgaccagtat ctgacatcag agaaaaaggt
     8641 cgatgactgg tttgaagcca agactcgcgg tttgtcgaat ttagccagga cccagcttaa
     8701 gcaaaagtgg ggatcaatgc agaagctgct ctccagcaag tcgcggctgg agcagatcgt
     8761 taacgacatt ttgctggata tggatacccg tccacgcctg atggacgggc gcggcaacgc
     8821 catgctggta tgcagcagta tctatcaagc ctgcaaggtt tacgagatgt tcagccagac
     8881 cgagctggca ggaaaagtag cgattgtgac cagctatcgc cccgatgccg ccagcatcaa
     8941 aggcgaagaa acgggcgaag ggttaacgga gaaactctct aaacacgcca cctatcgtaa
     9001 aatgttggct gactattttg aacaaagtga agagaaggcc gccgcacgca ttaccgagtt
     9061 cgagcgggaa gtgaaacagc gttttattga agagccgggg caaatgcgcc tgcttatcgt
     9121 ggtggataag ctgctgactg gctttgacgc gccgtccgcc acctatctct acattgataa
     9181 aaccatgacc gaccatacgc tgtttcaggc gatttgtcgg gtgaaccggc tggacggtga
     9241 ggataaagag tatggctata ttatcgatta taaagatctg ttccgttcgc tggataaagc
     9301 gattaccgat tacaccacgg gcgcgtttga tgattacgat cgggacgatg tggccgggtt
     9361 gctgaaagac aggctggagc aggcgcaact ggatctcgac agcgcgctgg agatggttcg
     9421 cagcttatgt gaacccgtca aaaccccgcg cagcctgcag gactatcagc actatttttg
     9481 cggtgaatca ggagagaatc agacttcgtt aaacgaaaaa gaggcgctgc gcctgacatt
     9541 ttaccagtgc gtcgcccgtc tgctacgcgc ttatgccagc atcgccaatg aaatgccgga
     9601 agcaggctac actgccgaag aggtagagag cattcgcgcc gaagtcgctg aattcgacaa
     9661 actgcgctat gagattaagc tgaccagcgg cgatcttctg gatatgaagc gctttgaacc
     9721 cgctatgcgt catttactgg atatgtatgt tcgcgccgat gatagcgaag tgctgatgga
     9781 ttttgaagag ttaagtctga ttgaactgat tgtcgagaaa ggcttggcgg cgaccgtcgc
     9841 cctgccggat gatatccgta ataatcagga tgcgatggcg gaaaccatcg aaaacaacgt
     9901 gcgcaaaacc atcgtggatg aaaacccggt taacccaaaa tactatggac gcatgtcgca
     9961 gctgctggat gaactgatta cccttcgccg acaaaatgcg ctcaattatc agcagtatct
    10021 ggaacgtatc cgcgatttgt gtaaacaagt catccgcccg gagcaaaccg ccggagcgtg
    10081 ctaccctgcc accatggata cacaagcaaa gcgcgccttc tatgataact tcggccacga
    10141 cgaggtactg gcgacccgca tagatacgac gattcgctat accaaaaggg cagagtggat
    10201 tggcgatcgt tttaaagaac gggaaattgc caacgcatta cgtgaagaga ccgccagcta
    10261 taatattgat attgatgagg tgatagcgct tgcccgtcag cagaaggaat atcactgatg
    10321 ccaatcatgc aaataggctc gttacacttg ctggtcaacc gtaaggcgat taagaatctg
    10381 catatcagcg tgctgccgcc tgatggcaaa gtccgcgttt cagcgccgga acatatgacg
    10441 gagacggcga ttcgtatggc ggttgcttca cgctttcgct ggatcagaaa acagcagcag
    10501 gatttcgcca gacagcctcg tcaatcagag agggaaatgg tcagcggaga gtgccactat
    10561 ctttggggcc gaagatatcg cctacttgtg attgagcgtc acgggcgtca cgaagtaaaa
    10621 gttgcgggaa ataataagct ccagctttat gttcaaccgg gaacccgtga tgaaaataag
    10681 gcattggcgt taagtgaatt ttatcgacat gaactaaaac agcaggtggc taagcttcta
    10741 cctgaatggc aggaccgaat cggtgttccc ctaacattct ggggcatcaa aaagatgaaa
    10801 accttttggg gcacctgtaa cactacaaca aaacgaatct ggctcaatct ggagctaatt
    10861 aagaagccgc cggagtgtct ggagtatatt ctggtgcatg aactggttca tcttctggaa
    10921 cgtcaccata atgctcgctt ccggttgcat atggatcggt ttattcctga ctggcgtgag
    10981 cgtcgggatt tgttaaataa tatgccgtta gcgtttgagg catgggagta ttgattgagc
    11041 ctgtaactgt tttttgtcca taaattaacc ttcatttgat gctggattga acatatcaaa
    11101 atcagacaat tacacaaatc tatgtacagg ctcaactcct gccatggaac gtagtactca
    11161 ccaataagta agcatcaata cggcgctccg ttaacgctta ctagcatggt tatctcctta
    11221 aaaaattgag gcgacaacta tgctgacaca ggggggtact caccgatctc cttcacggta
    11281 atacgcagtt cttcagaacc cgcctccgat accagcctgg cggtcgcccc ggcgcaacag
    11341 aataaatcaa ccatcgtcag actgcgatca ttcgtatgca tcgctgatga tgcgcccgaa
    11401 aaacgtgtca atatgagttc gccggaatat taccttacga aaaaacgtgt aaagctgtga
    11461 cagatgacgc gcaaaaaaaa tcccggcatt tgcgagcttt cccactattt cttgattcct
    11521 aaacggaaca tatcagttgg gaataaaggt tgtattatca cttcatcatt aaaaatgaat
    11581 a
//
LOCUS       DAZKGF010000152        11537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZKGF010000152 DAZKGF010000000
VERSION     DAZKGF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.152"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..658
                     /locus_tag="ACHOQV_003930"
     CDS             <1..658
                     /locus_tag="ACHOQV_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632220.1"
                     /translation="PKSFLTEKKHDSIDKNDASVSVKKVGLSEGDALKNTGVDKQEDD
                     YSRHVGTEESKSKVSGTEQNKNKGKTPPKVDRNVLVPSYVKFNFRGHKKCSRIFNELK
                     SHLTFDNTPNAISILLRIFIDLSVSTFVEENKLQFKEPHRTPGLHDKVKMCANFLREN
                     KKMTGSQCTAVITFSSQITKHDGSLQQYVHNPHLIPSKEAVNTEWDNFELLLSLIWSE
                     "
     gene            728..1612
                     /locus_tag="ACHOQV_003931"
     CDS             728..1612
                     /locus_tag="ACHOQV_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFP9632221.1"
                     /translation="MKFYTPLRYPGGKGKLSYFLKDVIEKNSLNDGAYAEPYAGGAGV
                     ALELLLEEYVRKIYINDADFAVYSFWSSVVSDTDELCRLISRAKIDMDEWSFHRYVIS
                     NPSEFTKLEVGFAAFFLNRTNRSGILKAGVIGGKSQNGKWKMDVRFNRADLISRIEKI
                     ANYNQRIVVTNLDALDFLDALNFMDNMGKVDKNKILLYLDPPYYIKGQGLYRNFYEHD
                     DHVLVMKKLTEINFPNWLVSYDNAKEIKDIYNGFPQVEYSLQYTAHLKKSGEEVMIFS
                     PEIIIPNTLKGVSMPTCA"
     gene            complement(1948..2982)
                     /locus_tag="ACHOQV_003932"
     CDS             complement(1948..2982)
                     /locus_tag="ACHOQV_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9632222.1"
                     /translation="MSKKKGKTPRPAAKTMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTPFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDND"
     gene            complement(2982..4754)
                     /locus_tag="ACHOQV_003933"
     CDS             complement(2982..4754)
                     /locus_tag="ACHOQV_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFP9632223.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            4928..5782
                     /locus_tag="ACHOQV_003934"
     CDS             4928..5782
                     /locus_tag="ACHOQV_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP9632224.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVVELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHDAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            5841..6914
                     /locus_tag="ACHOQV_003935"
     CDS             5841..6914
                     /locus_tag="ACHOQV_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP9632225.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRIRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            6918..7661
                     /locus_tag="ACHOQV_003936"
     CDS             6918..7661
                     /locus_tag="ACHOQV_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFP9632226.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDIRLLTETLELTATAD
                     MPDEVRAKLHKITGLFLRDGGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRVPRKTRSVTPAKRGRPKKKAS"
     gene            7761..8270
                     /locus_tag="ACHOQV_003937"
     CDS             7761..8270
                     /locus_tag="ACHOQV_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP9632227.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            8270..8473
                     /locus_tag="ACHOQV_003938"
     CDS             8270..8473
                     /locus_tag="ACHOQV_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFP9632228.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            8477..8758
                     /locus_tag="ACHOQV_003939"
     CDS             8477..8758
                     /locus_tag="ACHOQV_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9632229.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            8758..9255
                     /locus_tag="ACHOQV_003940"
     CDS             8758..9255
                     /locus_tag="ACHOQV_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFP9632230.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            9270..9695
                     /locus_tag="ACHOQV_003941"
     CDS             9270..9695
                     /locus_tag="ACHOQV_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFP9632231.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVATWAILATVIC
                     VVAGGVGVYATEYVLELYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRKACRQRLEGRELCQS"
     gene            9683..10108
                     /gene="lysB"
                     /locus_tag="ACHOQV_003942"
     CDS             9683..10108
                     /gene="lysB"
                     /locus_tag="ACHOQV_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFP9632232.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRASLDRANNVASEQQ
                     TTITMLKNQLHVAITRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACADASDCPQRLPESESLPDAGQ"
     gene            10008..10253
                     /gene="lysC"
                     /locus_tag="ACHOQV_003943"
     CDS             10008..10253
                     /gene="lysC"
                     /locus_tag="ACHOQV_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFP9632233.1"
                     /translation="MLCAGCTSARPAQTPVIVHNACPKVSLCPMPGSDPQKNGDLSAD
                     IRLLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            10216..10683
                     /locus_tag="ACHOQV_003944"
     CDS             10216..10683
                     /locus_tag="ACHOQV_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9632234.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSMSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            10676..11128
                     /locus_tag="ACHOQV_003945"
     CDS             10676..11128
                     /locus_tag="ACHOQV_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP9632235.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSTELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEETILAHLER"
     gene            complement(11200..>11537)
                     /locus_tag="ACHOQV_003946"
     CDS             complement(11200..>11537)
                     /locus_tag="ACHOQV_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632236.1"
                     /translation="YVYSDKVLLSFKNKTVELNKTYSLADCKTLQNVFIKDTLPEIAC
                     NKLLQLNEEDSEWLSQEIKDNNSHKKALLILSLVYFISGLLIFLSYTKFLYANKKVLE
                     YKASNKNHS"
BASE COUNT         2732 a         2853 c         3235 g         2717 t
ORIGIN      
        1 gcctaaatct tttcttacag agaaaaaaca tgattctatt gacaaaaatg atgcatctgt
       61 gagtgtaaag aaagtaggtc tttcagaagg tgatgcctta aagaatacag gtgtggataa
      121 acaggaagat gattattcta gacatgtagg cacagaagag agtaaatcta aagtttcggg
      181 tacggaacag aataaaaaca agggaaaaac tcctcctaaa gtagatcgta atgtgttggt
      241 gccatcttat gtgaaattta attttagagg gcataagaaa tgctctagaa tatttaatga
      301 gcttaagtcg catctgacat ttgataacac ccctaatgct atctcaatat tgttgagaat
      361 atttatagat ctctctgtaa gcacttttgt tgaagaaaac aagttacaat ttaaggaacc
      421 acatagaact ccagggcttc atgataaggt taaaatgtgt gctaatttcc ttcgggaaaa
      481 taaaaagatg acggggagtc aatgtacggc tgtaataact ttctcgagcc aaataactaa
      541 acatgatggt tcattgcagc agtatgtaca taacccacac ttaattccct caaaagaggc
      601 tgtcaacact gagtgggata actttgagtt gttattaagc cttatttgga gtgagtgaga
      661 tttgtctaca tctggacatg gatgtccaga aactgataaa ctacaaaatc aggaatgaga
      721 gggatttatg aaattttata ctccattaag ataccccggc gggaaaggta agttatctta
      781 ctttttaaaa gatgttattg aaaaaaactc gttgaatgat ggagcatatg cagaacctta
      841 cgctggtggt gcaggggtgg ctttagaatt attattggaa gagtatgtcc gtaaaatcta
      901 tataaatgat gcagattttg ctgtttattc tttttggtca tctgttgtta gtgatactga
      961 tgagctttgc cgacttatta gccgagcaaa gatagatatg gatgaatggt cttttcatcg
     1021 ctatgttatt tcgaatcctt ctgagtttac taagcttgag gttggctttg ctgcgttttt
     1081 cttaaataga acaaatcgtt caggtattct caaggcgggg gttataggcg ggaaatcaca
     1141 aaatggaaaa tggaaaatgg atgttcgctt taacagggcg gatttaattt caagaattga
     1201 gaagatagca aattataatc aaagaatagt agtaactaat ctggatgctt tggattttct
     1261 tgatgccttg aattttatgg ataatatggg aaaggtggat aaaaataaaa ttcttttgta
     1321 tttggatcct ccttactata ttaaagggca agggctttac aggaattttt atgagcatga
     1381 tgaccatgta ttggttatga agaaactcac agaaattaat ttcccaaatt ggcttgtttc
     1441 ttatgataac gctaaggaaa ttaaagatat ttataatgga tttccgcaag ttgagtattc
     1501 attgcaatat acagcgcatc tcaaaaaatc tggagaggaa gtgatgattt tttctcctga
     1561 aataattatt cctaataccc tgaaaggggt ctcaatgcca acttgcgcct gattttactt
     1621 attttttgtc agtaagtaat tagtttggtg catttatttg cattcatttt taaatgccag
     1681 ttctggtatc taacatcagt gctggcgcat tctaaccatt actgctgcac tgcatttaaa
     1741 gccctgcatg aagcgggcgg gcgaggcggg gaaagcactg cgcgctggcg gtggtgctga
     1801 ttttattttt tcagcgtctg agcgcgtcgt gacggcgctt agtctgcccg ttgaggcgtt
     1861 ggtgtgtctg cggggtgttt tgtgcggtgg tgagcgtgtg agggcgtgat gacggggtgt
     1921 aaaaaagccg cccgcaggcg gcgatgttca gtcgttgtca gtgtccagtg agtagttttt
     1981 aaagcggatg acctcctgac cgagccagcc gtttatctcg cggatcctgt cctgtaacgg
     2041 gataagctca ttgcggacaa agacctttgc cactttctca atatcaccca gcgacccgac
     2101 gttctccggc ttgccgccca tcaactgaaa ggggatgcgg tgcgcgtcca gcaggtcagc
     2161 ggcgctggct tttttgatat taaaaaaatc gtccttcgtt gccacttcac tgagcgggat
     2221 aattttaatg ccgtcggctt tcccctgtgg ggcatagaga aacagatttt taaagttatt
     2281 gcggcctttc gacttcacca tgttttcgcg aagcatttcg atatcgttgc gatcctgcac
     2341 ggcatcggtg acgtacatga tgtatccggc atgcgcgccg ttttcgtaat acttgcggcg
     2401 gaacagcgtg gccgactcat tcagccaggc agagttaagg gcgctgagat attccggcag
     2461 gccgtacagc tcctgattaa tatccggctc cagcaggtga aacacggagc cgggcgcgaa
     2521 aggtgtcggc tcgttgaagg acggcaccca ccagtaaaca tcctcctcaa caccacggcg
     2581 ggtatatttt gccggtgatg tttccagtct gatgacctta ccggtggtgc tgtagcgctt
     2641 ttccagaaac gcattaccga acaccagaaa atccagcaca aagcggctga aatcctgttg
     2701 cgaaagccac ggatgcggga taaatgttga ggccagaata ttgcgtttga cgtaaatcgg
     2761 tgagctgtga tgcacggcag cacgcaggct ttttgccaga ccggtaaagc tgaccggtgg
     2821 ctcataccat ctgccgttac tgatgcactc gacgtaatcc agaatgtcac ggcggtcgag
     2881 taccggcacc ggctcaccaa aggtgaatgc ctccattttc ggggcgctgg cggtcattgt
     2941 ttttgccgct ggtcgcggtg ttttcccttt tttcttgctc atcagtaaaa ctccagaatg
     3001 gtggatgtca gcggggtgct gataccggcg gtgagtggct catttaacag ggcgtgcatg
     3061 gtcgcccagg cgaggtcggc gtggctggct tcctcgctgc ggctggcctc ataggtggcg
     3121 ctgcgtccgc tgctggtcat ggtcttgcgg atagccataa acgagctggt gatgtcggtg
     3181 gcgctgacgt catattccag acagccacgg cggatgacgt cttttgcctt gagcaccatt
     3241 gcggttttca tttccggcgt gtagcggatg tcgcgtgcgg cgggatagaa cgagcgcacg
     3301 agctggaaca cgccgacacc gaggccggtg gcatcaatac cgatgtattc gacgttgtat
     3361 ttctcggtga gtttgcggat ggattcagcc tgagtggcaa agtccatgcc tttccactgg
     3421 tgacgctcaa gtattctgaa tttgccaccg gccaccaccg gcggtgccag taccacgcat
     3481 ccggcgctgt cgccacggtg tgacgggtcg taaccaatcc ataccgggcg ggagccgaac
     3541 ggattcgcgg caaacggcgc atagtcttcc cattcttcca gcgtgtcgac catgcagcgt
     3601 tgcagctcct cgaacgggaa caccgacgcc ttgtcgtcaa caaattcaca catgaacagg
     3661 tttttaaaat cgtcggcgct gttttcgcgt ttgagctgct caatgtcgaa cagcgtgcag
     3721 ccgcctttca gggcgtcctc aatggtgaca atctgccgcc actggccgtc cgcacagaga
     3781 agtccaccgg caagggcgtt atgactgacg tcgatttcca cgcgttcggc ggcgctggcg
     3841 cgtccccggt taaacagttc acccgaccag aacgggtagg cgtcgtgcgc cagcgtggac
     3901 ggggtggaga aataggtcga acgcaggtga ctctgtgagg ccatacctga tgccacctta
     3961 cgcagtacct gaaaattcgg gatccagaaa atctcgtcga cgtacaggtc gccgttatgg
     4021 ctctgtgcgg tgttggagtt ggtgccgaga aaaatcagtt ttgcgccgtt attgcccagg
     4081 acaatcgggt caccggtcag gtcaacgtca accagccggg caaaggcgat gatgtattcg
     4141 cggaacacat acgcctgcgt tttactggcc gacagaaaaa tctggttatg accggttttc
     4201 agggcgcgca gcagcgcctc gcgggaaaaa taaaacgtcg cgccaatctg gcgggatttc
     4261 aggatatcgc ggatgcggtg ctcaagcccg gcgcgatacc agtgcaactg atagtcgaaa
     4321 gactgctcaa agaaaatctg ctccagcttt tcgatggctt cgtcactgaa aaaattcttt
     4381 ttcggtttgc gacgcccacc tttgttgcgg ttagcaatgt tcggattaag gtctgcctcg
     4441 ttgccggtct ggctgtaacg gttgacccgt gccagtcgtt caatctggcg tcccagcagg
     4501 tcaatttcct tgaagtcccc gccggttttc tgcggtttga tgatgagctg ggtcagccgc
     4561 gcttccagac tcatttcgac acggctgatg ggggcaacgc tgtcccagcc gtcgcgctgt
     4621 ttccagctct gcaccgtcgg gcgtttcatc tgcaacatgg cggcaatctg cggcacggaa
     4681 aatccctgcc agtacagcag cgccgcctga cgacgcgggt cgtgtaaaag agtggtgtct
     4741 gtggtgatgg tcatgaatac ctcgccgtga taaatacacg gcaaggctac tgagtcgcgt
     4801 cccgcgattc gctaaggtgc tgttgtgtca gtgataagcc atccgggact gatggcggag
     4861 gatgcgcatc gtcgggaaac tgatgccgac atgtgactcc tctaatcact attcaggact
     4921 cctgacaatg gcaaaaaaag tctcaaaatt ctttcgtatc ggcgttgagg gtgacacctg
     4981 tgacgggcgt gtcatcagtg cgcaggatat tcaggaaatg gccgaaacct ttgacccgcg
     5041 tgtctatggt tgccgtatta acctggaaca tctgcgcggc atcctgcctg acggtatttt
     5101 taaacgttat ggtgatgtgg tcgaactgaa ggccgaaaag attgacgatg attcggcgct
     5161 gaaaggcaaa tgggcgctgt ttgcgaaaat caccccgacc gatgacctta tcgcgatgaa
     5221 caaggccgcg cagaaggtct atacctcaat ggaaattcag ccgaactttg ccaacaccgg
     5281 caaatgttat ctggtggggc tggccgtcac cgatgacccg gcaagcctcg gcacggaata
     5341 cctggaattc tgccgcacgg caaaacacaa ccctctgaac cgcttcaaat taagccctga
     5401 aaacctgatt tcagtggcaa cgcctgttga gctggaattt gaagacctgc ctgaaaccgt
     5461 gttcaccgcc ctgaccgaaa aggtgaagtc catttttggc cgcaaacagg ccagcgatga
     5521 tgcccgtctg aatgacgtgc atgacgcggt gaccgctgtt gctgaacatg tgcaggaaaa
     5581 actgagcgcc actgagcagc gcctcgctga gatggaaacc gccttttccg cacttaagca
     5641 ggaggtgact gacagggcgg atgaaaccag ccaggcattc acccgcctga aaaacagtct
     5701 cgaccacacc gaaagtctga cccagcagcg ccgcagcaag gccaccggtg gtggcggtga
     5761 cgccctgatg acgaactgct gaccggcgtc agtcagtccg ggaaaacctt cacgattaac
     5821 ccttaatttc aggaaaaact atgcgccagg aaacccgctt taaatttaat gcctacctgt
     5881 cccgtgttgc cgaactgaac ggcatcgacg ccggtgatgt gtcgaaaaaa ttcaccgttg
     5941 aaccgtcggt cacccagacc ctgatgaaca ccatgcagga gtcctctgac tttctgaccc
     6001 gcatcaacat tgtgccggtc agcgaaatga aaggggaaaa aattggcatc ggtgtcaccg
     6061 gctccattgc cagcaccacc gacaccgccg gtggcaccga gcgtcagccg aaggacttct
     6121 cgaagctggc gtccaacaag tacgaatgcg accagattaa cttcgatttt tatatccgct
     6181 acaaaacgct ggacctgtgg gcgcgttatc aggatttcca gctccgtatc cgtaacgcca
     6241 ttatcaaacg ccagtccctt gatttcatca tggccggttt taacggcgtg aagcgtgccg
     6301 aaacctctga ccgcagcagc aatccgatgc tgcaggatgt ggcagtcggc tggctgcaga
     6361 aataccgcaa tgaagctccg gcgcgcgtga tgagcaaggt cactgacgag gaagggcaca
     6421 ccacctctga ggtcatccgc gtgggcaagg gcggtgatta tgccagcctt gacgcactgg
     6481 tgatggatgc gaccaataac ctgattgagc cgtggtatca ggaagaccct gaccttgtgg
     6541 tgattgtggg acgtcagtta ctggcggaca agtatttccc catcgttaac aaggagcagg
     6601 acaacagcga aatgctggcc gctgacgtca tcatcagcca gaaacgcatc ggtaacctgc
     6661 cggcggtacg cgtcccgtac ttcccggcgg atgcgatgct catcacgaag ctggaaaacc
     6721 tgtccatcta ctacatggat gacagccatc gccgcgtgat tgtggaaaac ccgaaactcg
     6781 accgcgtgga gaactacgag tcaatgaaca ttgattacgt agtggaagac tacgccgccg
     6841 gttgtctggt ggaaaaaatt aaggtcggtg atttctccac accggctaag gcgaccgcag
     6901 agccgggagc gtaaccgatg acgagtcccg cacagcgcca catgatgcgg gtctcggcag
     6961 cgatgaccgc gcagcgggaa gccgccccgc tgcgacatgc aactgtctat gagcagatgc
     7021 tggttaagct ggccgcagac cagcgcacac tgaaagcgat ttattcaaaa gagcttaagg
     7081 ccgcgaaaaa gcgcgagctg ctgccgttct ggttgccgtg ggtgaacggt gtgctggagc
     7141 agggcaaagg tgcacaggat gacattctga tgacggtcat gctgtggcgt ctggataccg
     7201 gcgatattgc cggtgcgctg gagattgccc gttatgccct gaagtacggt ctgaccatgc
     7261 cgggtaaaca ccgccgcacc ccgccgtaca tgttcaccga ggaggtcgca ctcgcggcca
     7321 tgcgcgctca cgctgccggt gaatctgtgg atatccgcct gctgacggag acccttgaac
     7381 tgaccgccac ggctgacatg cctgatgaag tgcgcgcaaa gctgcacaaa atcaccggtc
     7441 tgtttctgcg tgatggtggt gatgccgccg gtgcgctggc tcacctgcaa cgtgcgacac
     7501 agctcgactg tcaggcaggc gtcaaaaaag agattgaacg actggagcgg gagctgaaac
     7561 cgaagccgga gccgcagccc aaagcggcca cccgtgtccc gcgtaagacc cggagcgtga
     7621 caccggcaaa acgtggacgc ccgaaaaaga aagccagtta acaaccgaat gcgccccgcg
     7681 ccagggcggc acgccggtca gtgacggtga attacctgac actgcaccgg cgtccaccgc
     7741 ccgacttttc agaggtagtc atgatgacgc tgattattcc gcgaaaggag gctcccgtgt
     7801 ccggtgaggg tacggtggtc atcccgcaac cggcaggcga cgagccggtg attaaaaaca
     7861 cgttcttttt tcccgatatc gacccgaagc gcgtccggga acgtatgcgc cttgagcaga
     7921 ccgtcgcccc cgcccgtctg cgtgaggcca tcaagtcagg catggctgaa acgaatgcgg
     7981 agctgtacga gtaccgcgaa cagaaaattg ccgccggttt tacgcgtctg gcggacgttc
     8041 cggcggacga catcgacggt gaaagcatca aagtttttta ctacgagcgc gccgtgtgtg
     8101 cgatggcgac cgcgtcgctt tatgagcgtt atcgcggcgt ggatgccagt gcgaaaggcg
     8161 acaagaaggc cgacagcatt gacagcacca ttgatgaact gtggcgggat atgcgctggg
     8221 cggtggcgcg tatccaggac aagccgcgct gcatcgtgag tcaaatctga tgaagacctt
     8281 tgcgctacag ggcgacacgc tcgacgccat ttgtgtccgg tattacgggc gcactgaggg
     8341 cgtggttgag accgtgctcg ccgcaaatcc gggactggct gaactgggtg cggtgctgcc
     8401 acacggcacc gccgtcgaac tgcccgacgt tcagaccgcg cccgtggctg aaactgtcaa
     8461 tctgtgggag taacgcatga cagcagaaga aaaaagcgtc ctgtcgcttt tcatgattgg
     8521 ggtgctgatt gttgtcggca aggtgcttgc cggtggtgaa cccatcaccc cgcgtctgtt
     8581 tatcgggcgc atgttgctcg gcggttttgt ctcgatggtt gccggtgttg ttctggtgca
     8641 gtttcctgac ctgtcactgc ctgcggtgtg cggcatcggt tccatgctgg gtatcgccgg
     8701 ttatcaggtg attgagattg ccattcagcg ccgctttaag ggcaggggga aaccgtaatg
     8761 ccggtaatta acacgcatca gaatatcgcc gcctttctcg acatgctggc cgtgtccgaa
     8821 gggacggcga atcatccgct gacgaaaaac cggggctatg acgtgatagt caccggactg
     8881 gacggaaagc cggaaatttt caccgactac agtgatcacc cgttcgcgca tggccgaccg
     8941 gcgaaggtgt ttaaccgtcg cggtgaaaaa tccacggcct ccggtcgcta tcagcagctt
     9001 tacctgttct ggccgcacta ccgcaaacag cttgccctgc cggatttcag tccgttgtca
     9061 caggacaggc tcgccattca gttgatccgc gaacgcggtg cactggatga catccgggcg
     9121 ggacgcattg agcgcgccat ttcacgctgt cgcaatatct gggcgtccct gccgggtgcc
     9181 ggttacggtc agcgtgagca ttcactggaa aaactggtca ccgtctggcg taccgccggc
     9241 ggcgtaccgg cttaaacgga gtaaacacca tgaagaaatt atccctttca ctgatgctga
     9301 acgtgtcgct ggcgctgatg ctggcactgt ccctgattta cccgcagagc gtggccgtca
     9361 gttttgtcgc cacctgggcg attctggcga cggttatctg tgtggttgcc ggtggtgtcg
     9421 gcgtgtatgc cacggagtat gtgctggaac tctacgggcg ggagctgccg ccggaatcgc
     9481 tggccgtgaa gattgtcacg tcgctgtttt tgcagccggt gccgtggcgc agacgggcgg
     9541 cggctctggt ggtgatggtg gcgacgttta tctcgctggt cgctgccggg tggattttta
     9601 ccgcgctgat ttatcttgtg gcgtcgctgt ttttccggct gatacgtaaa gcctgtcgtc
     9661 agcgtcttga ggggcgggaa ctatgtcaaa gctgatgatt gtgctggtcg tgttgttatc
     9721 gctggcggtg gccggtctgt ttctggtgaa acacaaaaat gccagcctgc gcgcctcgct
     9781 ggacagggcg aataacgtcg ccagtgaaca gcagacgacc atcaccatgc tgaaaaatca
     9841 gcttcatgtt gccatcacca gggcagacaa aaacgagctg gcgcaggtgg cactgcgtca
     9901 ggaactggag aacgccgcga agcgtgaagc acagcgcgag aaaaccatca cgaggttact
     9961 caatgaaaac gaagattttc gccgctggta cggtgctgac ctgcctgatg ctgtgcgccg
    10021 gttgcaccag cgcccggcct gcgcagacgc cagtgattgt ccacaacgcc tgcccgaaag
    10081 tgagtctttg cccgatgccg ggcagtgacc cgcagaagaa cggcgattta agtgccgata
    10141 tccggctgct tgagaacgcg ctggcacgct gtgccagcca ggtaaaaatg attaaacact
    10201 gtcaggacga aaacgatgct caaacccgac agcctgcgca gggcgctgac tgatgccgtc
    10261 acggtgctga aaaccagtcc cgagatgctg cggatattcg tggataacgg gagtattgcc
    10321 tccacactgg cgacgtctat gtcattcgaa aagcgttaca cgctcaatgt gattgtgacc
    10381 gactttaccg gtgattttga cctgctcatt gtgccggtgc tggcgtggct gcgggaaaat
    10441 cagcccgaca tcatgaccac cgacgaaggc cagaaaaagg gcttcacgtt ttatgcagac
    10501 atcaacaatg acagcagctt tgatatcagc atcagcctga tgctgaccga gcgcacgctg
    10561 gtcagtgagg ttgacggcgc gctgcatgtg aagaatatcc cggaacctcc gccgccggag
    10621 ccggttaccc gcccgatgga gctgtatatc aatggcgaac tggtgagcaa gtgggatgaa
    10681 tgagtttaag cgttttgaag accggctgac cggactgatt gagtcgctgt caccgtcagg
    10741 gcgtcgacga ctgagtaccg aactggcgaa acgtctgcgt cagagtcagc agcgtcgggt
    10801 gatggcacag aaagccccgg acggcacacc ctacgcgcca cgccagcagc agagcgccag
    10861 aaaaaagacc ggtcgtgtta agcgaaaaat gtttgcgaaa cttatcacca gtcgcttttt
    10921 gcatatccgc gccagcccgg aacaggcatc aatggaattt tacggcggga agtcaccaaa
    10981 aatcgccagc gtgcatcagt tcggcctgtc ggaagaaaac cggaaagacg gtaagaaaat
    11041 tgattatccg gcgcgtcccc tgctcggctt taccggtgag gatgtgcaga tgattgaaga
    11101 gactatcctg gctcaccttg agcgttagtt ttatccaggc agaggctgat gcgcaattaa
    11161 acattgagcg gccgtgctgg tcgctcaatg tttagaggtt tatgagtggt ttttatttga
    11221 tgctttgtat tctaaaacct tcttattggc gtaaaggaat tttgtatatg acaggaatat
    11281 aagcaggcct gaaatgaaat agacgaggga tagtattaat aatgcttttt tgtggctgtt
    11341 attatcttta atctcctgac ttaaccattc ggagtcctcc tcgtttagct gtaagagctt
    11401 attgcaggcg atttcaggaa gtgtgtcttt tataaatacg ttttgcaggg tcttgcaatc
    11461 ggcaaggcta taagttttat taagttcaac tgttttattt ttgaaggata aaagaacttt
    11521 gtcactataa acatagt
//
LOCUS       DAZKGF010000153        11527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZKGF010000153 DAZKGF010000000
VERSION     DAZKGF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.153"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(214..1020)
                     /gene="trpA"
                     /locus_tag="ACHOQV_003947"
     CDS             complement(214..1020)
                     /gene="trpA"
                     /locus_tag="ACHOQV_003947"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFP9632237.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNLAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(1020..2213)
                     /gene="trpB"
                     /locus_tag="ACHOQV_003948"
     CDS             complement(1020..2213)
                     /gene="trpB"
                     /locus_tag="ACHOQV_003948"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFP9632238.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2225..3586)
                     /gene="trpCF"
                     /locus_tag="ACHOQV_003949"
     CDS             complement(2225..3586)
                     /gene="trpCF"
                     /locus_tag="ACHOQV_003949"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFP9632239.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPAHIATVYRNYASAISVLTDEKYFQGSFDFLS
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRHLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3587..5182)
                     /gene="trpD"
                     /locus_tag="ACHOQV_003950"
     CDS             complement(3587..5182)
                     /gene="trpD"
                     /locus_tag="ACHOQV_003950"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFP9632240.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHVPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDSQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5182..6744)
                     /gene="trpE"
                     /locus_tag="ACHOQV_003951"
     CDS             complement(5182..6744)
                     /gene="trpE"
                     /locus_tag="ACHOQV_003951"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFP9632241.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCEDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQSLLNVPNEEREAMFFGGLFSYDLVAGFED
                     LPQLAAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRSLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6766..6859)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6836..6880)
                     /gene="trpL"
                     /locus_tag="ACHOQV_003952"
     CDS             complement(6836..6880)
                     /gene="trpL"
                     /locus_tag="ACHOQV_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFP9632242.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            7018..7899
                     /gene="rnm"
                     /locus_tag="ACHOQV_003953"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             7018..7899
                     /gene="rnm"
                     /locus_tag="ACHOQV_003953"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFP9632243.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAISPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HYGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7896..8516
                     /gene="yciO"
                     /locus_tag="ACHOQV_003954"
     CDS             7896..8516
                     /gene="yciO"
                     /locus_tag="ACHOQV_003954"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP9632244.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8544..10433
                     /gene="yciQ"
                     /locus_tag="ACHOQV_003955"
     CDS             8544..10433
                     /gene="yciQ"
                     /locus_tag="ACHOQV_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFP9632245.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYPKNTEIPFYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVIRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAILREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLICFPSLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKEKIHLEDHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWEGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAICAPEIARNGVLFSLQTHLSCGAYTSLLGRSYSGGGSGAGGG
                     GGGGW"
     gene            10646..11521
                     /gene="rluB"
                     /locus_tag="ACHOQV_003956"
     CDS             10646..11521
                     /gene="rluB"
                     /locus_tag="ACHOQV_003956"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFP9632246.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHC
                     QVSGGRRSGGRNNNG"
BASE COUNT         2718 a         2916 c         3071 g         2822 t
ORIGIN      
        1 taagtgactt agagaaaatt attgatttta ctggtgttat gttgcgggat taatttgttt
       61 gattatatta ctgttgggcg gaaaaaagac gtaagttgac gttcgacagg ggcaagcgaa
      121 acggtaaaaa gataaataat aaatgaattt aggatttttc cagcttcgtt aaagaaagtt
      181 aaaatgccgc cagcggaact ggcggctgtg ggattaactg cgcgtcgccg ctttcatcgg
      241 ttgtacaaaa gctttcagtg ccgccagcat tttctctggc tcattaatat gttgctcgat
      301 gattttaacg atggccgaac cagaaatcgc gcccgcagct cctgcatcaa tcgctgcttt
      361 tacctgatcc ggggcggaaa taccaaagcc ctgcaatgga ggcgcagcat tgtattcttt
      421 cagcttcgtg accagatgat tgaggggtaa cgcggcgcgg ttttctgcgc cggtcacgcc
      481 cgcacgtgac aataaatagg tgtatccacg accgtaggag gctatctggc gcagcaaatc
      541 atcgtcggca ttcggcgggc agataaagat aggtgcaaga ttatgacgca acgcggcctg
      601 gcggaaggga gcggactctt ccactggcac atcggcaacc agcaccgaat cgacgccgac
      661 tttctcgcac tgggcataaa actcatcaat gcctttgtta aacaccaggt tggcatacat
      721 cagcaggcca atgggaatgg tcgggtgttt ctggcgaatc agtgccagca tttcaaaaca
      781 ttgtgccgga gtcacacctg ccgcaaaggc gcgcagagtg gcgttttgaa tcgtcgggcc
      841 atccgccagt gggtcggaga aggggatacc taactccagc gcgtcagcac cggcttcaat
      901 tagcgtgtcg ataattttca gcgactgctc aatgcccgga tcaccgagcg tgacgaaagg
      961 aacgaatgcg ccttctttgc gctccttcaa ctgggcaaac agagattcgt agcgttccat
     1021 cagatttccc ctcgtgcttt caaaatatcg tgaacggtga agatgtcttt atcgccgcga
     1081 ccggaaaggt taaccaccag tagctgctct ttttccgggt tttcgcgcat cattttcagc
     1141 gcatgggcca gggcgtgtga ggactccagc gccgggataa tcccttcgtg caggcagagc
     1201 gttttgaagg cttccagtgc ttcatcatcg gtaattgaca cgtaatcagc gcgtccagtg
     1261 ctgttaagat acgcgtgttg tgggccgaca gacgggaaat ccagtccggc ggagatggag
     1321 taagattctt caatctgccc gtcttcggtt tgcatcatcg gcgctttcat accgaaatag
     1381 atgcccacgc gaccgtgttt taacggtgcg ccgtgctcgc cagtttcgat accgtgacca
     1441 cctggctcca caccaatcag gccgacgttg gtttcattga tgaaatcagc aaacatgccg
     1501 atggcattcg aaccgccgcc aacacaggcg ataacagcat ccggcaggcg accttctctt
     1561 tccagaatct gcgctttcgt ttcttcgcca atcatccgct gaaactcacg cacaatggtc
     1621 gggtaaggat gcgggccagc cgcggtgccc agcatatagt gcgcggtttc ataactgccg
     1681 gaccagtcgc gtagcgcctc attacaggca tctttcagcg tcgcggaacc gctatgcacc
     1741 gggatcactt ccgcacccat taaacgcata cgaaaaacgt taggcgactg gcgttcaacg
     1801 tctttggcac ccatataaat gcgacatttc aggccgagca gggcgctggc aagggccgac
     1861 gccacgccat gctgaccggc tccggtttcg gcgatgattt ccgttttacc catccgcttc
     1921 gccagcaacg cctggccgag cacttggttg gttttatgcg cgccgccgtg cagcaaatct
     1981 tcgcgcttga gatacagcgt ggtgttagtg ccagccgtaa tgttctggca tttggtgagt
     2041 gcggttggac gcccggcata gtttttcagc aggtcgttga actgagcctg aaattcagga
     2101 tctttttgcg cgctaacaaa agcttcttcc agctggcgca gagcaggcat caggatttgt
     2161 ggcacgtaca tgccgccaaa ctcaccaaaa tacgggttaa gtaatgttgt cattgttcct
     2221 ttccttaata tgcgcgcagc gtctggaaaa ccgaggccaa aagacgtgcg tctttgatgc
     2281 ccggttgcga ctctacagca gaattaaaat caagtccggc gcagccggtt tgtgccgctt
     2341 ccacgcagtt atctgcgcct aagccccccg ccagcagaac gttgccaagc gattgaccgt
     2401 ttaacagtga ccagtcgaaa cgttgaccgc ttccaccctg gccgttgtct aaaacatatt
     2461 tatcaacgtg ctgaagctcg cgggcgggca gggtttcacc gacgcttaat gctttccaga
     2521 tggcaacgtg tgctggcaga gcttcacgca gcgtatcgat atacagctga tcttcattac
     2581 catgcagttg cactgccgcc agcgataaca ccttagcttt gtccaccaca tcggcaatat
     2641 cgtgattgcg gaacacgcca acatactgca acggtgccgc agccatcact tcctgcgcct
     2701 gttcaacgtt gacgcaacgc ggtgatgtcg caacaaaaat caacccaccg taaatcgcgc
     2761 ccgcgtcata agctgcttta gcatcttgcc cacgcgtcag gccacatact ttattctcac
     2821 ccagcaatac ccggcgcacg gcggcgttca aatcgtcatg ggccatcaac gccgaaccaa
     2881 tcagaaaacc gttagcgaag tggcttaact cgcgcacctg agcgtaagta ttgatgccgg
     2941 attcgctgat taccgtcacg ttgtgcccca gtttcggcgc aagctcgcgg gtacggttga
     3001 gatcaatcga caaatcacgc agatcgcggt tgttgatgcc aacgaccttt gcccccaatg
     3061 caatggcgcg ctccagttcc tcttcattac tgacttcggt cagcacaccc atctccagac
     3121 tgtgggcgac ggcggcaagg tggcgatatt gttcgtcatc cagtactgaa agcattaata
     3181 agcaggcatc ggcctggtaa tagcgcgcca gatagatctg gtaagggtcg ataataaagt
     3241 ctttacataa aatcggctgc ggggcgattt ggctgacgat ggagaggaaa tcaaagctcc
     3301 cctgaaaata tttctcatca gtcagcaccg aaattgccga agcgtaattt ctgtagacgg
     3361 ttgcaatgtg tgccggatca aaatcatcgc ggatcacgcc ttttgacggt gacgcttttt
     3421 tacactccag aataaacgcc gtgcgtgcac cctgtagcgc atcataaaaa tgtcgcgtgc
     3481 tcggctgaac ctcattctga aaactggcca gcggttgctg ctgtttgcgg gcttctaccc
     3541 aaatcgcctt gtctgcgacg attttcgcta aaacggtttg catcatttac cctcgtgccg
     3601 ccagtgcggt gactctgtcg taagcggaac cactgcgcag tacctcaaga acggtttgcg
     3661 cattggcttg cagatcttca tggccatgca ggcgcattaa catggcgacg ttcgccgcga
     3721 cggctgcttc atgggcggcg tcgcctttac cttgtaacaa gcgtgttaaa atgtcacggt
     3781 tttcttccgg tgttccgcct gccagttgct cctggtggta gggtgtcagg ccaaaatctt
     3841 ctgcagttag ctgatagctc ttaatttcac cgtcatgcag ttcggcaacg attgtcggcg
     3901 cgtgtaatga aacttcatcc atcccgccgc tgtgcactac cgcagcgcgt tgatacccca
     3961 gcacgcgcaa ggtttcggca atcggcagca ccagttccgg actataaaca ccaattagcg
     4021 ccagcggcgg atgcgccggg ttaatcaatg gccccaacac gttgaacagg gtgcgggttt
     4081 tcagttgctg gcgaaccggc atcgcgtggc ggaatccggt gtgatatttc ggcgcaaaga
     4141 ggaaacagac gcctaactca tccagcgcct ggcgcgattt atcggcgttc atatcaagat
     4201 taataccgaa cgccgccagc agatcggacg agccggattt actggagacg ctgcggttac
     4261 cgtgtttcgc cactttcagc ccacaggccg cggcgacaaa cgcactggcg gtagaaatat
     4321 tgatgctgtt gctgccgtca ccgccagtac cgacgatatc ggcaaacaga taatccgggc
     4381 gcgggaacgg cgcggcgttt tccagtagcg cggttgctgc tccggcgatc tcgttcgggt
     4441 gctcgccgcg aattttcatg ctcaccagcg ccgccgccag ttgttccggc ttcagctcgc
     4501 cacgcaccac cgctgaaaac agctggtgac tttcttgttg gctaagcgtc tgtgcctgat
     4561 acagtttttc cagaatcggt tgcagcgtgt tggttggctc aagtttctgc tgcgcccagg
     4621 ccagcgtttg ttccagcagg cgagcgccct gggtagtaag aatggattcc ggatggaact
     4681 ggaaaccgca aacgcgatct gcatcgtgac gcaccgccat caccatgcca ttaaaatggg
     4741 cgttgatggt taaacctgcc gggatattac tgccaaccag cgagtgataa cgcgccaccg
     4801 gcaatgggtt tgttaatccg gcaaacatcg cctgactgtc atgttcaatg ctcgacgctt
     4861 taccgtgaag aatttcgccc gcctgaccga catagccccc gtaagcttcg acaattgcct
     4921 gatgtccgag gcaaatgcca ataattggca gcttgccacg caagcgggtg aggagttccg
     4981 gcatacaacc ggcttcgctc ggcacaccgg ggccaggaga aagcatcagc accggattgc
     5041 tcatcgtcgc caggcgttca attaaggtct gcgccggaac atggttgcgg taaatcacca
     5101 cgttatgacc attgctgcgc aactgatctg ccaggttgta cgtaaaagag tcgatattat
     5161 cgagcagcag aatgtcagcc atcagaacgt ctcctgtgca tgatgcgcgg tggcaatagc
     5221 gcgcagtaca gcgcgggctt tattacgggt ttcgtcggct tccgactgcg gaacagaatc
     5281 aaggactacg ccagcaccgg cttgcacggt ggcgataccg ttttccacca gcgccgagcg
     5341 gatcacaatg caggtgtcga gatcgccatg cgcggtaaaa taacctaccg cgccgccgta
     5401 gctgccgcgt cgacgacctt ctgcttcggc aattaactgc atagcgcgca ctttcggcgc
     5461 accgcttaat gtccccatat tcatacaggc gcggtaagcg tgcagggcgt cgagatcgtg
     5521 gcgcagctca ccgaccacgc gggagacgag gtgcatcaca taagaataac ggtcaacttt
     5581 ggtgagatcg gcgacgtagc gactaccggg agtgcaaata cgcgccaggt cattacgggc
     5641 gagatccact agcatcagat gttcagaaag ctctttatga tcggtacgca tttccagttc
     5701 gatgcggctg tcgaggtctc tgtccagtga accatcggca cgacgaccgc gtggacgtgt
     5761 tccggcaatc gggtagatct caatctggcg gctggtggcg tcatacttga gcgaactttc
     5821 cggcgacgcg ccaaacaggg tgaaatcatt atcctgcata aaaaacatgt acgggctggg
     5881 attactcttt ttcagcacgt aataggccgc cagcggtgac gggcagggca gagagaaacg
     5941 gcgagatggc accacctgga aaatttctcc ggcgcgaatc gctttttgca gcgaacgcac
     6001 tacgccaccg aactcttcat cactctggtt acattcacaa cgcatatgcg gcacggaaac
     6061 caccggcagc ggcggcgcgg cttcggtcag ttgctgacgt agttcgttca ggcgagcagt
     6121 gagacgttgt ttttcttctt cattcggagc aaacaggctg gcctgaatac gggtgctttt
     6181 tttctgatgg tcaatcacca tcagcgtttc agcgagataa aaacagaaat caggacagtt
     6241 attttccgct gccagttgtg gtaaatcttc aaatcccgcc acaaggtcat aagagaacag
     6301 accgccgaag aacattgctt ctcgttcttc attcggtaca ttcaacagac tctgcaataa
     6361 gcggaaagcg tcaaaaaccg aaagggagca taagcgagcg tcttcatcca gcagtggact
     6421 gacagggggg aagcgcagca cacggcagtt tggtgattgt tcattttcca cacccgcagg
     6481 cagggcgtta tccagtagtg ccagcagggc ttcgccattg ccggaaagtg cctggattgt
     6541 gacagtgtca cctaaagctg taatgcgcag cgcactgtct accagcagca ggctttttaa
     6601 atcatctttg ctgtcgatat ctgcggattc cagtagcagc gttgctggac gatcctcaca
     6661 caactggtga aaaagcgcgg tgggattgtc gcgataagcg ccttcgcagg ttagcagttc
     6721 gagagtcggt ttttgtgttt gcattgttat tctctaattt tgttcaaaaa aaagcccgct
     6781 cattaggcgg gctgggtatc tgattgcttt acgcatggtg aatacactgc ccgtttcagg
     6841 aagtgcgcca ccaacctttc agtacgaaaa ttgctttcat tgtcgatacc ctttttacgt
     6901 gaacttgcgt actagttaac tagttcgatg attaattgtc aacagctcat ttcagaatat
     6961 ttgccagaac cgttatgatg tcggcgcaaa aaacattatc cagaacggga gtgcgccttg
     7021 agcgacacga attacgcagt gatttacgac ctgcacagcc ataccacagc ttccgatggc
     7081 tgcctgacgc cagaagcatt ggtgcaccgt gcagtcgaga tgcgcgtcgg caccctggcg
     7141 atcaccgacc atgacaccac agctgcaatc tcaccagcaa gagaggaaat ttcacgttcg
     7201 ggactggcgc tgaatcttat tcccggcgtg gaaatttcca cggtctggga aaatcatgaa
     7261 attcatattg tcgggctgaa tattgatatt actcatccgc taatgtgtga gttcctcgcg
     7321 cagcagacag aacggcgcaa tcagcgggcg cagctgattg ccgaaagact tgaaaaagcg
     7381 caaatacctg gggcactgga aggcgcgcaa cgactggcgc agggcggtgc ggtgacgcgc
     7441 ggtcattttg ctcgtttcct tgtggagtgc ggcaaagcca gttcaatggc ggatgtcttt
     7501 aaaaagtatc tggcgcgcgg gaaaaccgga tacgttccgc cacagtggtg tacaatagaa
     7561 caagctattg atgtcattca tcattctggc ggtaaggcgg tgctagctca tcctgggcgg
     7621 tacaatctta gtgctaaatg gctgaaaaga ttggttgcgc attttgccga acactacggc
     7681 gacgcgatgg aagtcgcgca gtgccagcaa tcgcccaatg aacgcaccca gctggcgaca
     7741 cttgcgcgtc agcatcattt atgggcatca caaggatctg attttcatca gccatgcccg
     7801 tggatcgaac tgggtcgtaa actctggctg cccgcaggcg ttgaaggcgt ctggcagcta
     7861 tgggaacagc cgcagaacac cacagagagg gaattatgag ccagtttttt tatattcatc
     7921 ctgataaccc gcagcaacgc ctgatcaacc aggcggtgga gatcgtgcgt aaaggcgggg
     7981 tgattgttta tccaactgat tccggctatg cgctcggctg taaaattgaa gataaaaacg
     8041 cgatggagcg tatttgtcga attcgccagc tgccggacgg tcacaacttt accctgatgt
     8101 gtcgcgatct ttctgagctc tcgacctatt cattcgtgga taacgttgcg tttcgtttga
     8161 tgaaaaacaa cacgccgggc aactacacct tcatcctgaa agggacgaag gaggtgccac
     8221 gccgtctgtt gcaggaaaaa cgcaaaacca tcggtatgcg tgtgccttct aacccgattg
     8281 cgcaggcgct gcttgaagca ttgggcgaac cgatgctttc cacatcactg atgctgccag
     8341 gcagcgaatt taccgaatcc gatccggaag aaattaaaga ccgtctggaa aaacaggtgg
     8401 acttgattat tcacggcggc tatctcgggc agaaaccaac aacggttatt gatcttactg
     8461 atgatacgcc ggtcgtggtg cgtgaaggcg taggtgatgt gaagcctttc ttataacaaa
     8521 ttgcgttcca cggatggaag actatggcag ggatatatcg ctgcattttg ctgttgatag
     8581 ttgggctttt tttctcttcg ttgagttatc cgaaaaacac ggagatccct ttttatgaag
     8641 aagggatctc gctctttgat gttgaagcca ctctgcaacc ggatggggtg ctcgacatca
     8701 aagaaaatat tcattttcag gcgcgaaatc agcagattaa gcacggattt tatcgtgatt
     8761 taccacgact ctggatgcag cctgatgggg acgctgcact gctgaactat catattgttg
     8821 gcgtcatccg tgatggtatt cctgaaccct ggcatcttga ctggcatatc gggttaatga
     8881 gtattgtcgt gggcgataaa caacgtttct tgcctcaagg cgactatcat tatcaaattc
     8941 attatcaggt taaaaatgct atcctgcgtg agggggattc tgatctgcta atctggaacg
     9001 tgaccggtaa ccactggccg tttgaaattt ataagaccct attttcactc aagttgcctg
     9061 atattgcggg taatccattt agcgaaatcg atctctttac tggagaagag ggcgacacat
     9121 atcgaaatgg ccgcatcctt gaggacggaa gaattgaatc tcgcgatccg ttttatcgtg
     9181 aagatttcac ggtcctctac cgctggcctc acgcattact tggtaatgcc ccggctccac
     9241 aaacgacgaa tattttcagt catcttcttt taccctcaac gtcatcgttg ttaatttgct
     9301 ttccgagtct gttcctggcg tgtggatggt tatatctctg gaagcgcagg ccgcaattta
     9361 cgccggtaga tgtgattgaa accgatgtca ttccgccaga ttacacgccc ggcatgttac
     9421 gtcttgatgc gaaactggtt tacgacgata aaggtttttg cgccgatatc gtaaatctga
     9481 ttgtgaaaga aaaaattcat ctggaagatc attatgacaa gaaccagcaa atcctgattc
     9541 gtgttaatga aggcgcgacc agaaataatg cggtattact gcccgcagag cagttattac
     9601 tggaagcgtt atttcgtaaa ggcgataagg tcgttcttac ggggagacgc aacagagtct
     9661 tacgcaaggc atttttacgg atgcagaaat tttatctgcc gcgtaaaaag tcttcgtttt
     9721 accgacctga tgcgtttttg caatggggcg gaatggcaat attggcggtc attctctacg
     9781 gtaacctgag tcccgtaggc tgggaaggaa tgagtctggt gggcgatatg tttattatga
     9841 tctgctggct tcttcctttt ttattttgtt cccttgagct tttgtttgcc cgcgatgatg
     9901 acaagccttg cgttaatcgt gtcatcatca ctttgttttt accactgatt tgttcaggtg
     9961 tggcttttta ctctctctac atcaatgtcg gagatgtatt cttttactgg tatatgccag
    10021 cgggttattt tagcgctgtt ttcctgaccg gttatctcac tggcatgggg tatatttttc
    10081 tgccaaagtt tacccaaact gggcagcaac gttatgccca cggtgaagct atcgttaact
    10141 atcttgcgcg taaagaggca gcaacacaca gtgggcggcg taggaaaggg gaaacacgga
    10201 aactggatta cgctttgtta ggttgggcta tatcggcaaa tttggggagg gaatgggcgt
    10261 tacgcatcgc cccttcgctt tcttcggcga tttgcgctcc agagattgcc cgtaacggcg
    10321 ttttattctc attacagacg cacctgagtt gcggggccta taccagtttg ttggggcgga
    10381 gctattctgg tggtggttct ggcgctggtg gcggaggcgg tggtggctgg taatcaagct
    10441 gatgttaatc gaaacaagct ttaaccataa agatagtgta gtatgttgcg cctcaaagcc
    10501 aggcaagtta aaacgtcgag tcgtttactt aaggcctgaa gagttcaaac agactttact
    10561 atcagtgagt cagatgtaaa ttctgactca ctgttgcaca aaagcatatc agacgcctgg
    10621 gaaggcgaca ccaaaaggaa gctctatgag cgaaaagcta cagaaagtgc tggcgcgtgc
    10681 cggccacggt tctcgccgtg aaatcgaatc tattattgaa gccggtcgtg tgagtgttga
    10741 tggcaaaatt gctaaactcg gcgatcgcgt tgaaattacc cctggattga aaatccgtat
    10801 cgatggtcac ctgatttcgg tacgtgagtc cgctgaacaa atttgtcgcg ttctggccta
    10861 ttacaaaccg gaaggtgagt tgtgtacccg taacgatccg gaagggcgtc caacggtgtt
    10921 tgaccgtctg ccaaagctgc gtggcgcacg ctggattgcc gtaggtcgcc tggacgttaa
    10981 tacctgtggt ctgctgctgt tcactaccga cggtgaactg gcaaaccgtt taatgcatcc
    11041 aagccgtgaa gttgaacgtg aatacgccgt gcgcgtattt ggtcaggttg acgacgcgaa
    11101 actgcgtgat ttgagtcgtg gcgtgcagct ggaagatggt ccggcagcct ttaaaaccat
    11161 caagttcagc ggcggcgaag ggatcaacca gtggtacaac gttactctga ccgaaggccg
    11221 taaccgtgaa gttcgtcgtc tgtgggaagc ggttggtgtg caggtaagcc gcctgatccg
    11281 tgttcgttac ggtgatatcc cactgccgaa aggtctgcca cgcggtggct ggacggagct
    11341 ggacctcgcc cagactaact atctgcgcga actggtggag ctaccgccag aaaccagctc
    11401 taaagtcgct gtagaaaaag accgtcgtcg tatgaaggcg aatcagattc gtcgtgcggt
    11461 gaaacgtcac tgtcaggtga gcggcggtcg tcgttctggt ggacgtaata ataacggtta
    11521 atttaag
//
LOCUS       DAZKGF010000154        11498 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZKGF010000154 DAZKGF010000000
VERSION     DAZKGF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.154"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..325)
                     /locus_tag="ACHOQV_003957"
     CDS             complement(<1..325)
                     /locus_tag="ACHOQV_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9632247.1"
                     /translation="MIVCYVILVNRPFETEIAGKKLFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVMQNLSLQKTL
                     PQQFSE"
     gene            complement(423..616)
                     /locus_tag="ACHOQV_003958"
                     /pseudo
     CDS             complement(423..616)
                     /locus_tag="ACHOQV_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            complement(1036..1218)
                     /locus_tag="ACHOQV_003959"
     CDS             complement(1036..1218)
                     /locus_tag="ACHOQV_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632248.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     IYLLQKMNITDKTKYK"
     gene            1157..3244
                     /gene="iha"
                     /locus_tag="ACHOQV_003960"
     CDS             1157..3244
                     /gene="iha"
                     /locus_tag="ACHOQV_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFP9632249.1"
                     /translation="MRITTLASVVIPCLGFSASSMAAEDVMIVSASGYEKKLTNAAAS
                     VSVISQEELQSSQYHDLAEALRAVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGV
                     RQGGSGDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKNA
                     DKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQIRGSTQQRQGSSVTSLS
                     DTSATRIPYPTESQNYNLGARIDWKATEQDVLWFDMDTTRQRYDNQDGQLGSLTGGYD
                     HTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKSDKWGLAGKPREL
                     KESNLILNSLLLTPLGESHLLTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEW
                     HLTDVLALTAGSRYEHHDQFGGHFSPRAYLVWDVTDAWTLKGGVTTGYKAPTMGQLHK
                     GISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLSGLNANITGFITDFSNKIVSYS
                     IDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTQSEQRDGDNKGAPLSYT
                     PEHMVNAKLNWQITEEVASWLGARYRGKTPRFTRNYSSLSAVQKKVYDEKGEYLKAWT
                     VVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSSLYAGDYFQTGSSTTGYVI
                     PERNYWMSLNYQF"
     gene            complement(3462..3612)
                     /locus_tag="ACHOQV_003961"
                     /pseudo
     CDS             complement(3462..3612)
                     /locus_tag="ACHOQV_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            4106..4348
                     /locus_tag="ACHOQV_003962"
     CDS             4106..4348
                     /locus_tag="ACHOQV_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632250.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            4639..5007
                     /locus_tag="ACHOQV_003963"
     CDS             4639..5007
                     /locus_tag="ACHOQV_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632251.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            5011..5223
                     /locus_tag="ACHOQV_003964"
     CDS             5011..5223
                     /locus_tag="ACHOQV_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632252.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQTFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEAESS"
     gene            5220..5492
                     /locus_tag="ACHOQV_003965"
     CDS             5220..5492
                     /locus_tag="ACHOQV_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurotensin receptor R8"
                     /protein_id="HFP9632253.1"
                     /translation="MTDITDRHTLRRMSWSELTTAAQEAELNRDYERARIVWSFALHV
                     ATTTINKNLAIAHIRRCDTLLHKSKTVPGNNPRGRSVCLRPQHPRR"
     gene            complement(5801..6632)
                     /locus_tag="ACHOQV_003966"
                     /pseudo
     CDS             complement(5801..6632)
                     /locus_tag="ACHOQV_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            6690..6878
                     /locus_tag="ACHOQV_003967"
                     /pseudo
     CDS             6690..6878
                     /locus_tag="ACHOQV_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(6947..8173)
                     /locus_tag="ACHOQV_003968"
     CDS             complement(6947..8173)
                     /locus_tag="ACHOQV_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9632254.1"
                     /translation="MKDMQAMLPQTFYIKPGQFIWSYLGTFLFMLGGCIENSWLSAWL
                     NTQGFDQSQIGQIFAAYGIVVAVTSWLSGVCVDMFGPRKVMLAGFSVYLIASIAFLNF
                     AIPSHDFSTILISYMLRGVGYPLVCYSFLVRLTIQLDNHQQGIGTSLFWVVYNLGFTI
                     IGPVVAASLIPVLGHINVMWAGIGVALLGGIFMLVFERNAFILKPRTTSVFKELSAGI
                     SIMYQRPRIGLAVIVKTINGLGTYGFVVVLPLFLLDKQFTLEEWASIWGITFISNQIF
                     NIIFGWMGDKIGFRRTIQIFGSLLTGFATLIVYWVPIVWGHNYVAFMLAMCLWGAGLA
                     GFVPMTPLVPMMAPDKKGAANSAVNFGSGLGNFVGPALVSILTGFGTGVVMYTMAGLY
                     LFSGILVQFLKVPGEK"
     gene            complement(8246..9268)
                     /gene="iolG2"
                     /locus_tag="ACHOQV_003969"
     CDS             complement(8246..9268)
                     /gene="iolG2"
                     /locus_tag="ACHOQV_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-chiro-inositol-2-dehydrogenase IolG2"
                     /protein_id="HFP9632255.1"
                     /translation="MQKLRCGIIGLGRVGKMHVENMYLLPQLDIVCAADSFIEEMSDW
                     LSSVNITSGYKDYQEMLQRDDIEAVFIFTSTDMHEEIVTAAVQAGKHIFCEKPLSMRE
                     DEKASLAVLRAVKEKGVKLQIAFNRRFDPQFREVYELVRRGKIGQPQMIKITSRDPEL
                     LPHDLIKRIGGLIFDFTMHDFDMARFIMQDEVSEVYVKGNTLIDPTLKNIDDVDTLVV
                     MLTFRNGGYALIDNSRRAVYGYDQRVEVFGSAGMAYADNVSESAVKVYNSQNCIMKNP
                     LPDFALRYREAYRTEILHFIDSVLNHTPIVCTGEDALLAQRIAIAAKQSLKSGLPVKI
                     KTDIQL"
     gene            complement(9441..11381)
                     /gene="iolD"
                     /locus_tag="ACHOQV_003970"
     CDS             complement(9441..11381)
                     /gene="iolD"
                     /locus_tag="ACHOQV_003970"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFP9632256.1"
                     /translation="MKTIRLTMAQALVRFLDNQYIEVDGREIKFVKGIFAIFGHGNVV
                     GLGQALEEECGQLSVHQGRNEQGMAHIATGFARQMRRQQIYACTSSVGPGAANMITAA
                     ATATANRIPLLLLPGDVYASRQPDPVLQQIEQEHDLTLSTNDAFRAVSRYWDRIIRPE
                     QLMSACISAMRVLTDPADTGAVTLCLPQDVQGEAWDYPDYFFARRVYRLERHVPTELM
                     LNDAVALILRHQRPIIICGGGVKYSQSEQELLRFAEQYHLPIAETQAGKGALSSAHPL
                     NVGGIGETGSLAANLLAQEADLIIGVGTRYTDFTTSSKWIFQNPDVRYLNINVSRFDV
                     FKLDGVQLQADARVALTQLCERLAPEGYISQWGKTIHRVRSQYLAEVDRVYAAEYSGK
                     DFKPEIVDHMDTQKVFEEFNEITHSWLTQTRVLGVLNRMLPENALVVAAAGSLPGDLQ
                     RIWQSRGDNDYHVEYGYSCMGYEVNAALGAKLAQPEREVYSFVGDGSFMMLHSELVTS
                     VQMGKKINVILLDNMTNGCINNLQMEHGMDSFFTEFRFHKQDNGRQEGGLIPVDFARI
                     AEGYGCKSYRVTSIGQLLEALEDARKQTVSTLIDIKVLPKTMAHKYLSWWRVGGARVS
                     RSERIQAVARMLDEHIGQARQY"
BASE COUNT         3176 a         2885 c         2562 g         2875 t
ORIGIN      
        1 gctcagaaaa ttgttgtggc aatgtctttt gcagactgag attctgcatg acattatgta
       61 acagagcgat gtgagagaag gttgttttaa tgaacggaac agaaacagca ctatcagagt
      121 gctggatcct gtcggcactt tccatatatt ttctgacaat gcattcgtca atccggagct
      181 cagtaacatt accgggatgg cgcaggaaaa aggactggct gatgttatct gtcaacagga
      241 cgtcattttt ctgaaaaaat agctttttcc cggcaatctc tgtttcaaac ggacggttaa
      301 caagaataac gtaacagaca atcatcacac atactccctg attagcataa aaacaacgat
      361 atcaaaagac tagacgcttt ttcaggaaaa atcttatttg tccaggatta ttcttacgag
      421 accggcgctt caacggtacc gtcacatttt ttacaggctt tttttacccg ttctgtgctg
      481 atcactttca gggagctgct caccagttcc agttgttcag cgctgacttc cccagataat
      541 ccagctcacc gccacactcc gggcaacagc tttcttcagg ctccagccgg tgtatttcac
      601 ggggaaggtg tgccggtgtc agcgctagtg gtataagacg gttctccctc atcgtttgtc
      661 cttagtattg tcagcgccaa caatgtgggc tggcattata aatgccgtcg aggcaatcgt
      721 tcgcctcccc agtgggcaat ccaaatggtg attgtcacgg ttagacgttt attttgaagg
      781 tcgactgaat aaggtgataa cgctgtagta ttttcacgtg acacagaatt ctgaacgttc
      841 tctcttacaa tatttcaatg tttctgtccg tattcaccgg aagaagtcat cgaccaaatc
      901 ataccagtcg tctcactcca ctgaccattc atggttgaca ggtggtgaaa atccgcttct
      961 gcagtaacca ttttttattc gcaaaaccga acacgccatt gcggatggct gttaactggc
     1021 atgccgaggc agtcattatt tatatttagt tttgtcagta atattcattt tttgtaaaag
     1081 gtaaatataa attattctca ttattgtttg tatttgtgta ttgtcttgcc gattaacatg
     1141 atcggagatt agtaatatgc gaataaccac tctggcttcc gttgtcattc cctgtctcgg
     1201 gttttcagcc agcagcatgg cagcagagga tgtgatgatt gtctcggcat ccggctatga
     1261 gaaaaaactg accaacgcag ccgccagtgt ttccgtaatc agtcaggagg aattgcagtc
     1321 cagccagtac cacgatctgg cggaggcact gagagctgtg gagggtgtgg atgttgaaag
     1381 tggtacgggt aaaaccggtg ggctggaaat cagcatccgt ggaatgcccg ccagttacac
     1441 gctgatactg attgatggtg tccgtcaggg cgggagcggg gacgtgacgc ctaacggttt
     1501 ttctgccatg aatacagggt tcatgcctcc tctggccgcg attgagcgta ttgaggttat
     1561 cagggggccg atgtccacac tgtatggctc tgatgcgatg ggtggtgtgg tgaatatcat
     1621 taccagaaag aatgcagaca aatggctctc ttccgtcaac gccgggctga atctgcagga
     1681 gagtaacaaa tggggcaaca gcagccagtt taatttctgg agcagtggcc ctcttgtgga
     1741 tgattctgtc agtctgcaga tccgcggcag cacacagcag cgtcagggct catcggtcac
     1801 atcactgagc gacacatcag ccacgcgtat tccttatccc acggagtcac agaattataa
     1861 tcttggtgcg cgtattgact ggaaggctac ggagcaggat gtgctctggt ttgatatgga
     1921 taccacccga cagcgttacg ataaccagga tggtcaactg ggtagcctga cagggggata
     1981 tgaccatact ctgcgctatg agcgaaacaa aatttcggct ggctatgatc atactttcac
     2041 cttcggtacc tggaaatcgt atctgaactg gaacgagacg gaaaataaag gtcgtgagct
     2101 tgtacgtagt gttctgaaga gcgataaatg ggggcttgcc ggtaaaccgc gggagcttaa
     2161 ggaatctaac cttatcctga attcattgct gcttacccct ctgggagaat ctcatctgct
     2221 tactgtgggg ggagagtatc agagttcgtc catgaaagat ggggttgtcc ttgccagcac
     2281 aggtgaaact ttccgacaga aaagctggtc agtatttgct gaggatgagt ggcatctcac
     2341 ggatgtactt gcgctgactg caggcagtcg ctatgaacat catgatcaat tcgggggaca
     2401 cttcagtccg cgtgcatatc tggtctggga cgtgaccgat gcctggacgc tgaaaggcgg
     2461 tgtgaccacg ggatataaag cccccaccat gggacagtta cataagggga tcagcggtgt
     2521 atccgggcag ggacgtacta atctactcgg taaccccgaa ctgaagccgg aagagagtgt
     2581 aagttatgag gcgggggtat attacgataa cctctccggt ctgaatgcca atataacagg
     2641 ctttataaca gacttctcca acaagattgt ctcttattcc attgatgata acaccaatag
     2701 ctatacaaac agcggaaagg cccgcttgca tggtgtggaa ttcgccggca cattgccgct
     2761 gtggtcagag gatgtcactc tgtcgttgaa ttacacctgg acccaaagtg aacaacgtga
     2821 tggtgataac aaaggtgcac cgctgagtta tacccctgaa cacatggtga atgcgaaact
     2881 gaactggcag atcaccgaag aggtggcatc atggctgggt gcccgttatc gcgggaaaac
     2941 accacgtttc acccggaatt attcgtcact gagcgctgta cagaagaaag tgtatgatga
     3001 gaaaggagaa tacctgaaag cctggacggt ggtggatgca ggtctgtcgt ggaagatgac
     3061 ggatgccctg acgctgaatg ctgcggtgaa taacctgctc aacaaggatt acagtgacgt
     3121 gagcctgtac agtgccggta agagttcgct gtatgccggt gattacttcc agacgggatc
     3181 atcaacaaca ggatatgtga tacctgagcg aaattactgg atgtcgctga actatcagtt
     3241 ctgataataa caaaacgcta tcactgacgg tagaatacgt tgccactgca actcctggcg
     3301 gaacagtggc aacgtattag gttaagtgca tttccgatcc gctaatgaga tttcgttacc
     3361 aacaactaat atcgtcacag gaaatgcacg gattattttt aacttatcat ttacatactt
     3421 gtccagagtg taagcgcacc gtgaaggacg tggggtaaaa attagtttac agagagagtg
     3481 acgttccagg gcaacaactc tttcatgcgg ttggcaggcc aggtgttgat tacactgatc
     3541 acgtggcgta gccacgtttc cggctcgatt ccgttaagtt ttgcagctac cgatcaggct
     3601 gtacatcact gccgcactat cgctcgtcat ctcaaagtcc ggtctcgtca gcaggaaggt
     3661 atcattctct cccgccattt ttccgggggc ccggtcagat aagtcccttt gtctatcgct
     3721 gactcctggc tcataacccg gttagcagaa tgcagggtca ccacctcgcc acgaccgaat
     3781 ccggataagt caattgcacc ttctcaatcg ccattttgtc acaattaagt ttgataaagc
     3841 aggtcacctc tgcactgaat ctgctccagt gcctcattgg tctccgtccc cccctttaaa
     3901 ctgccggata agactgctgc acagcgcctt ccccggaatc aaatcacttt ccctgtccgc
     3961 cacaatccgc ttcatgctac ctcgtatttt gtttcagaga ccggaatctg ccagaaagtg
     4021 actacgcgtg cacatatcgg ttttctgggt tacatgcatc gtgttatttt ctgaaatgca
     4081 aaataataaa aaacaaggac acataatgaa ggatgaaata cacagtacca gcatcaaccc
     4141 ggatgttgtt gagcagtttc tggcccttat gaacacacat tgtaaatcct cagaagaggc
     4201 gctcgccatg ttgagtacgc tttgttacaa cctgatggtg acatgtggca tccattccat
     4261 ggacagccag atgctcaatg ggcatgggat atccatgcaa cttcagggga ttgatattgc
     4321 cgaaaggcca ggtaagaata ttcactaacc tgaacagggc ctgaaaacgc tgtcttttca
     4381 ggcctctgca ggcaaaaact gatgacaaca aaatttctgc gtttgtaccc caccgatgaa
     4441 aaagacaaaa actcacaccg ggcttctagc cagtaaagat aaaacccgac gtgtcagcct
     4501 ctacgaaacg ccaacggcat ggtgtatcag ggggcaggag tgctacagca aaagtacagg
     4561 acgccgctgt ggcagccacg acagtctgag cagactgcgc ctagacagca ttaagcctgt
     4621 tgaatgagtg gtggcaccat gaaagataac aacacaaccg ccaccccaga agcctggaaa
     4681 atgcgttttc tggccaacgg gacatccggc acatggtttt acactgacga cattgcagtc
     4741 actgacgcgt ggcttcgcgc gcacccggat gatgtggagg ttatccctct ctgccggata
     4801 ccaccccgtc agcattccca tgaacaacat attgaagaac tccgcgcagc cctggcccgg
     4861 atgctgcata tgcatcatat gatgctggaa accacgcatt acagggaaaa catcaccgat
     4921 gcccgttgta tccgtgaaat gcatgaagct ccattacagg cgctcaatat tctggccaga
     4981 tacccgcata aaaaggacga ggtgtgacgc atgtggctac tgattttctg gctttcatct
     5041 ccctttaatg tctcttccgg tgatgcccgg cagcccacac tggcggtgca cagccagaca
     5101 tttcataccg aacgggcctg tcgcgatgca ttgcgcgcca ttaaaaagga gaatgttggc
     5161 gaattttctc tgcggggcgt ttgtgtttct gacggaaaca cgaaggaggc agagagctca
     5221 tgacggatat tactgacaga catacacttc gccgcatgtc ctggtcggaa cttaccacgg
     5281 cagcgcagga agcagaatta aaccgtgatt acgagcgtgc cagaatagtg tggtcattcg
     5341 ccttacatgt tgcaacaacc acgattaaca aaaatctggc catagctcat attcgacgct
     5401 gcgatacttt gttgcataaa tcaaaaacag tacccggaaa caacccgaga ggccggtcag
     5461 tgtgcctgag acctcaacat ccccggagat gactgacatt ccggactgac gcagttgctg
     5521 catatctgtg gcaatacgtt gtatccagcc agacaggcta tcccgcgtag tgacaattaa
     5581 cccgctttcc tgcaccagct tcagccgggc cgtcggggat tttaccagcc ggaaatatca
     5641 gtaaaacaac atatcattat gccatggtgt acgcggccag tgtatgctta cttaaggaat
     5701 gagggataaa ttggatatta ttacttcact ttgctttttt atgagattca tcacataagt
     5761 ggggttttac tgttaaataa tctgaattta tttgatatcc gtaaagcaga tgtctcagtt
     5821 cactgtttcc aacttttgtt attcctctgg ggcgaatgct gtttccgctg gaatattcac
     5881 ctggcaccag tcccagaaaa gccatgagct gttcaggatt ggaaaatcgc gaaaaatcac
     5941 ccagttcagc aaccagggtt attgcgatta gctgaccaac acctttaaga gcctgcaact
     6001 ggcagaccag attgcacaga gatcactcag gtaacaggcg agatatttcc tgatcgagtt
     6061 gaagtatgcg atcttcaatc tgctccagat tctgacaata atgttgaaag caatctgttg
     6121 agatagcaga ccaaatgatt gattaactaa ccaggttcga tgtttgccgg ttcagtgctt
     6181 cctggcataa cgtttgtctg ttcgtgtcac gttttgcagc ggcacgggct ctgattaaat
     6241 cccgcatagc ttcatgagtc agctcaggga gccagactgg gggaacctca ccatccctca
     6301 gtaacctggc gagagaaatt acgtcccgat ggtcattttt gatcctgtct gccggtgatt
     6361 tgggtatacg ggaagggggc aataaccagg cattttatat tcatcactgt gagttgatga
     6421 tatagcccaa acccacaggg accaccttca tagcaggaaa gaatgtttcc gtaaggtccc
     6481 tgcaatttct gacataatcg atgtacagcc aaggcttcgt tgttaatcgt accataataa
     6541 cgaacttcgc caagccattc aggtgtagca atcgccaagg tttctttatg aacatccagc
     6601 ccgacgtaag ctgcaatatt ttccgaatcc atgatgacct ctggagtatc ccaccaatgc
     6661 tatagtgagt atataaaggt catatcatct acgtgttctt cggcaacgat cacagtggag
     6721 agcgtggagc gctactgtac gggctgattg gcacctgccg actgaacagt atcgatccgg
     6781 aagcgtatct gcgccatatt ctgagcgtac tgccggaatg gccttccaac cgtgttgacg
     6841 aactgctgcc atggaacgta gttctcaccg ataaataagc gtcaatacgg cattcgatta
     6901 atgcacttac agctaaatgg agtttttgaa caatatttga cgttatttat ttctcaccag
     6961 gaactttaag aaactgaacc aggatgccac taaataaata gagtcctgcc atagtataca
     7021 taaccacgcc tgtaccaaaa cctgtcagaa tggataccaa cgcaggtccg acaaaatttc
     7081 ccaggccaga accgaaattc accgctgagt ttgctgcccc cttcttatcc ggtgccatca
     7141 tcggtaccag cggcgtcatc ggcacaaatc ccgccagccc cgctccccac aggcacattg
     7201 ccagcataaa agcaacgtaa ttatgccccc acactatcgg cacccagtag acaatcagcg
     7261 tggcaaaccc ggtcagaaga ctgccaaaaa tctggatagt acggcgaaaa cctattttat
     7321 cccccatcca gccgaaaata atgttgaaaa tctgattaga aataaaggtt ataccccaga
     7381 tactcgccca ctcctcaagg gtaaactgtt tatccagcaa aaacagcggc aacaccacta
     7441 caaaaccata ggtgccgagt ccattgattg ttttaacaat cactgccaga ccaattcggg
     7501 gtcgttgata cataatggaa atacctgccg acaactcttt aaagactgac gtagttcttg
     7561 gcttcagaat gaacgcattg cgctcaaaga ccagcatgaa aatcccccca agcagtgcaa
     7621 ccccaatccc ggcccacata acattaatat gcccaaggac aggaattaac gaagcagcta
     7681 ctaccggacc aatgatggta aaacctaagt tatatactac ccaaaacagc gacgttccaa
     7741 ttccctgttg atgattatcg agttgaatgg tcagtcggac aagaaaggaa tagcagacca
     7801 acggataacc tactccccgc agcatatagc taatgagaat tgtactaaaa tcatggctgg
     7861 gaatcgcaaa attaagaaac gcgatagaag cgatcaggta aacggagaaa ccagcaagca
     7921 taacctttct ggggccaaac atatccacac agacaccgga taaccaggaa gttacggcaa
     7981 ccacaattcc gtaggcggca aatatctgtc cgatctgaga ctgatcaaat ccctgagtat
     8041 tcagccaggc agatagccaa ctattttcaa tacagccgcc caacataaat aaaaatgttc
     8101 ccaggtagct ccagataaac tgacctggct ttatataaaa tgtctgaggc aacattgcct
     8161 gcatatcttt cattttataa ccctcagtac ggataatctc tcgctcgtaa tagtgcgaga
     8221 gacccttcgc tatttacaaa tgcaactaaa gttgaatatc agtttttatc ttcactggca
     8281 gtccactttt taatgattgt tttgcagcaa tggcaatacg ctgcgccaat aatgcatctt
     8341 caccagtaca gacaatgggt gtatgattca gaacagaatc aataaagtgt aatatttccg
     8401 ttctataagc ttcacgatag cgtagcgcaa aatcaggcaa aggatttttc atgatacagt
     8461 tctgactgtt atataccttc accgccgatt cactgacatt gtcggcatac gccatccctg
     8521 ctgaaccaaa gacctcaacc cgttggtcgt agccataaac cgcccgacga ctattatcaa
     8581 ttagcgcata accaccgttt ctgaaggtca gcatcaccac gagagtatct acgtcatcaa
     8641 tatttttcag agtaggatca atcaatgtat tgcctttaac ataaacctcg ctaacttcat
     8701 cctgcataat aaagcgagcc atatcaaagt catgcatagt aaaatcaaat atgagtccac
     8761 ctatcctctt gatcaaatcg tgcggcaaca actctggatc acgtgaggtg atctttatca
     8821 tctgaggctg accaatcttg cctctgcgca ccagttcata cacctcccga aactggggat
     8881 caaaacgacg attaaaagca atctgcagtt tgactccttt ctctttaacc gcccttagca
     8941 ccgcaaggga agctttctca tcttctctca tgctgagagg tttttcgcag aaaatatgtt
     9001 tacccgcctg gactgctgcc gtaacgatct cttcgtgcat atctgtcgag gtgaaaataa
     9061 acaccgcctc aatatcatca cgctgtaaca tttcctgata atctttatat cctgaggtta
     9121 tatttaccga actcagccag tcgctcattt cctcaataaa actgtcagca gcacagacaa
     9181 tatctaattg aggaagtaaa tacatattct caacatgcat tttccctacc cggccaagac
     9241 caataatccc acatcgaagc ttttgcattt atacctcgtc atatcatgat atagtaaata
     9301 taaaacgaca aaagaaaatc ccctttatcc aacacggtac gaaagacttt attttgtagt
     9361 ttcaacgaac aaatactttt accgtaaaaa acgtcaggtt gcagaaaggc gccctcctgc
     9421 aaccttaaaa actgaattaa ttagtactgt cgcgcttgtc caatatgctc atcaagcata
     9481 cgagctactg cctggatgcg ttcgctacgc gacacgcgag cgccaccaac acgccaccag
     9541 ctcagatatt tatgcgccat tgttttaggc agtactttaa tgtctatcaa cgtactcacc
     9601 gtctgtttac gtgcatcctc cagagcttca agcagttgtc caatgctggt gacgcgatag
     9661 cttttacagc catatccttc ggcaatccgg gcaaaatcca caggaatgag tcccccctcc
     9721 tgacgaccgt tatcctgttt atggaaacga aactcggtaa agaaactgtc cataccgtgt
     9781 tccatttgaa gattatttat acaaccatta gtcatgttat ccagcaaaat aacattaatt
     9841 ttcttcccca tctggaccga agttaccagc tcagaatgca gcatcataaa tgagccgtcg
     9901 ccaacgaagc tatatacctc ccgctccggt tgcgccaact tcgcccccaa tgcggcattg
     9961 acctcatagc ccatacagga atagccatat tctacgtggt aatcattatc gccacggctt
    10021 tgccagatgc gctgcagatc gcctggcaga cttcccgccg cagcaaccac cagcgcgttt
    10081 tctggcagca ttcgatttag tactccaaga acgcgcgtct gtgtcagcca tgaatgagta
    10141 atttcattga actcttcaaa cactttttga gtatccatat gatccacaat ttccggtttg
    10201 aaatccttac cgctgtattc cgctgcatag acgcgatcca cttccgccag atattgagaa
    10261 cgaacacgat ggatcgtttt cccccattgt gatatgtaac cttccggagc cagtcgttcg
    10321 caaagctgcg tcagggcaac acgggcatca gcctgcaatt gcactccatc cagtttgaag
    10381 acgtcaaaac ggctgacgtt tatattcaga tagcgcacat caggattctg gaaaatccat
    10441 ttagaagagg ttgtgaaatc ggtataacgc gtacccacac caataatcag atcggcctcc
    10501 tgagccagca aattcgccgc cagcgatccg gtttcaccaa tcccaccaac attcagcgga
    10561 tgcgcagaac tgagcgcacc tttaccagcc tgcgtttcag caatcggcag atgatattgt
    10621 tcggcaaatc gcagcagttc ctgttcagac tgcgaatact tcacaccccc accgcaaata
    10681 ataattggtc tctggtggcg aagaataaga gcaactgcat cattcagcat tagctctgtt
    10741 ggcacgtggc gctcaagacg atagacccga cgtgcaaaaa aatagtctgg ataatcccag
    10801 gcttctccct gaacatcctg cggcaaacaa agcgtcacag ctcccgtatc agccggatcg
    10861 gtaagcaccc gcatcgcgct aatgcaggcg ctcattagct gttccggacg gataatgcga
    10921 tcccaatagc gactcactgc acgaaaagca tcattggtgc tcagcgtcag gtcatgctcc
    10981 tgttcaatct gctgcaacac aggatcgggt tgacgcgatg catacacatc tcctggtagc
    11041 agaagcaacg ggatacggtt cgccgtcgca gtcgccgccg ccgtgatcat attagcggca
    11101 ccaggcccta cggaggaggt gcaggcataa atctgctgac gacgcatctg acgcgcaaat
    11161 cctgttgcta tatgtgccat accctgttca ttgcgtcctt gatgaacgct aagttgacca
    11221 cactcttctt ccagagcctg ccctaatccc acgacattcc catggccaaa aattgcgaaa
    11281 atacctttta caaacttgat ttcgcgaccg tccacttcaa tgtactgatt atcaagaaag
    11341 cgcaccaaag cctgcgccat agtcaacctg attgttttca tgcactccac ctatcttatc
    11401 aaccgtttga ctgtcgttac ctacgtacgc actgctacag ccccgggcat aaaacactca
    11461 ccacaagaaa aaaattccgc aaacgcaatt atatgaaa
//
LOCUS       DAZKGF010000155        11492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZKGF010000155 DAZKGF010000000
VERSION     DAZKGF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.155"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..926)
                     /gene="pyrD"
                     /locus_tag="ACHOQV_003971"
     CDS             complement(<1..926)
                     /gene="pyrD"
                     /locus_tag="ACHOQV_003971"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFP9632257.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIA"
     gene            1163..1738
                     /gene="ssuE"
                     /locus_tag="ACHOQV_003972"
     CDS             1163..1738
                     /gene="ssuE"
                     /locus_tag="ACHOQV_003972"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9632258.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            1731..2690
                     /gene="ssuA"
                     /locus_tag="ACHOQV_003973"
     CDS             1731..2690
                     /gene="ssuA"
                     /locus_tag="ACHOQV_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFP9632259.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            2687..3832
                     /gene="ssuD"
                     /locus_tag="ACHOQV_003974"
     CDS             2687..3832
                     /gene="ssuD"
                     /locus_tag="ACHOQV_003974"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFP9632260.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            3844..4635
                     /gene="ssuC"
                     /locus_tag="ACHOQV_003975"
     CDS             3844..4635
                     /gene="ssuC"
                     /locus_tag="ACHOQV_003975"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFP9632261.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            4632..5399
                     /gene="ssuB"
                     /locus_tag="ACHOQV_003976"
     CDS             4632..5399
                     /gene="ssuB"
                     /locus_tag="ACHOQV_003976"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP9632262.1"
                     /translation="MNTARLNQGTPLLLNAVSKQYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(5605..8217)
                     /gene="pepN"
                     /locus_tag="ACHOQV_003977"
     CDS             complement(5605..8217)
                     /gene="pepN"
                     /locus_tag="ACHOQV_003977"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFP9632263.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVRWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQLLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            8483..9685
                     /gene="pncB"
                     /locus_tag="ACHOQV_003978"
     CDS             8483..9685
                     /gene="pncB"
                     /locus_tag="ACHOQV_003978"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP9632264.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQYLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9854..11254
                     /gene="asnS"
                     /locus_tag="ACHOQV_003979"
     CDS             9854..11254
                     /gene="asnS"
                     /locus_tag="ACHOQV_003979"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP9632265.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2626 a         2972 c         3040 g         2854 t
ORIGIN      
        1 gcaatctttt cacgcgcagc gataaccgaa tcaatgccgc caacaccgat gatcggtaag
       61 cgaccgttta attcctgtga caagcggcga ataatttctg tgctttttaa ctgaagcgga
      121 cgaccactta agccaccggt ttgatcacaa tttttcattc cctgaaccag agaacgatcg
      181 agcgtggtat tggttgcaat cacgccatca atattatggc gaactaaact atcggcaacc
      241 tggatcaatt cttcttcaga aagatccggc gcgatcttca ctgcgatcgg cacatattta
      301 tggtgcatcg cttgcaaatc attttgctta tttttaatcg cggttaagag atcatccagc
      361 gcttcgccat attgcagtgt gcgtaatcct ggggtatttg gcgatgaaat attgatggcg
      421 atatatcccg cataggcata gattttttcc atacaaatca gatagtcatc tttaccctgc
      481 tccaccggcg tatctttatt tttgccgatg ttaataccca gaacgccgtc ataatgggct
      541 ttttttacgt tctctacgag gttatcaacg ccaagattat taaagcccat acggttgatc
      601 aaaccttcgg catctaccag acgaaagaga cgcggcttgt cattacctgg ctggggacgt
      661 ggcgtgacgg taccgatctc gatcgatcca aatcccatcg cgcctaacgc gtcaatgcac
      721 tccccgtctt tatcaagacc ggctgccaga ccaagcggat ttttaaacgt caggcccatg
      781 cagttaacag gtttcgcagg cactttctgc cgcaccagtg cttcaaacgg cgttccggta
      841 atacggcgta attgctgaaa agtaaactca tgagcgcgct ctggatcgag ctggaaaagg
      901 gctttacgaa cgaaggggta gtacatgaac tctcctggat tcccggtgtg caaaccgggg
      961 gcgtattatg tgcgagttcg gaccaaaagg gaattgacct gcggcaataa atagcaaacg
     1021 ttttcttatt gcgctttttt tatgcttttt ttggcttttc ttcctcagat aaatcatttc
     1081 gactcgcgga cgccctctgt cagactgacc gaattgttat caatgttaac aaaacaagaa
     1141 caattggtta taaggagaga gtatgcgtgt catcaccctg gcgggtagtc ctcgctttcc
     1201 ttctcgctcc agctccttgc tggaatatgc gcgggaaaaa ctaaatggcc tggatgtaga
     1261 ggtttatcac tggaatttgc aaaacttcgt cccggaagat ctgctttatg ctcgtttcga
     1321 tagtccggca ctcaagacct tcaccgaaca gctgcaacag gccgatgggc tgattgtcgc
     1381 cacgcctgtg tataaagccg cctattccgg cgcgctgaaa accctgctcg acctgctgcc
     1441 agaacgtgcg ttgcaaggca aagtggtgct gccgctggcg acaggcggca ccgtggccca
     1501 tctgctggcg gtcgattatg cccttaagcc agttttaagt acgctgaaag cccaggagat
     1561 cctgcacggc gtgtttgccg atgactcgca agtaattgat taccaacaca aaccccagtt
     1621 cacgccaaat ctgcaaactc gtcttgatac cgcgctagaa actttctggc aggcgttgca
     1681 ccgccgcgat gttcaggttc ctgaccttct gtctctgcga ggtaatgccc atgcgtaaga
     1741 tcattaaact ggcgctggcg ggtttactta gcgtctctac acttgctgtt gccgcggagt
     1801 cttcgccaga ggcgttacgt ataggctatc aaaaaggcag tattggtatg gtgctggcaa
     1861 aaagccacca gttactggaa aaacgctatc cgcaaacaaa aatctcctgg gtggagttcc
     1921 ccgctggccc acagatgctg gaggcgttaa acgttggcag tatcgatctc ggcagtaccg
     1981 gggatattcc gccaatcttt gcccaggctg ccggggctga tttggtatac gtgggcgtcg
     2041 agccaccgaa acccaaagcc gaagtgattc tggtggcaga aaacagcccg atcaaaaccg
     2101 tagccgatct taaaggtcac aaagttgcct ttcagaaagg ttccagttcg cacaatcttt
     2161 tactgcgagc ccttcgccag gccgggctta aattcacgga tattcagccc acttacctga
     2221 cgcctgccga tgcccgcgcc gcgttccagc aaggtaacgt tgacgcctgg gctatctggg
     2281 atccctacta ctccgctgca ttattacagg gcggcgtgcg ggtgctgaaa gacggcaccg
     2341 atctcaatca aactggatcg ttttatctgg cagctcgtcc gtatgcagaa aaaaacggcg
     2401 cttttattca gggtgtgctg acaaccttta gtgaggccga tgcgttaacc cgcagccagc
     2461 gcgaacaaag catcgcttta ctggcaaaaa cgatgggctt accggcaccg gttattgcct
     2521 catatctgga tcatcgtcct cccaccacca tcaaaccgtt gagcgctgaa gttgccgcct
     2581 tacagcagca aacggcagat ctgttttatg aaaaccgtct ggtgccgaaa aaagtcgata
     2641 ttcgccagcg catctggcag cccactcaac tggaaggaaa acaattatga gtctgaatat
     2701 gttctggttt ttaccgaccc atggtgacgg gcactatctg ggaacggaag aaggttcacg
     2761 tccggttgat cacggttatc tgcaacaaat tgcgcaagcg gcggatcgtc tgggttatac
     2821 cggtgtgctg atcccaacag gacgatcgtg tgaagatgcg tggctggttg ccgcgtcgat
     2881 gatcccggtg acgcaacggc tgaagtttct tgtcgccctg cgtcccagcg taacctcacc
     2941 taccgttgcc gcccgccagg ccgccacgct tgaccgtctc tcgaatggtc gtgcgttgtt
     3001 taatctggtc acaggcagcg atccacaaga gctggcaggc gacggcgtgt tccttgatca
     3061 tagcgagcgc tacgaagcct cggcggaatt tacccaggtc tggcggcgct tattgctggg
     3121 ggaaaccgtc aatttcaacg gtaaacatat tcatgtgcgc ggagccaaac tgctcttccc
     3181 gccgattcaa cagccgtatc ctccgcttta ctttggcgga tcgtcagatg tcgcccagga
     3241 gctggcggca gaacaagttg atctctacct cacctggggc gaaccgccgg aactggttaa
     3301 agagaaaatc gaacaagtgc gggcgaaagc tgccgcgcat ggacgtaaaa ttcgtttcgg
     3361 tattcgtctg catgtgattg ttcgtgaaac caacgacgaa gcgtggcagg ccgccgagcg
     3421 gttaatctcg catcttgatg atgagactat cgccaaagca caagccgcat tcgcccggac
     3481 ggattccgta ggacaacagc gaatggcggc gctacataac ggcaagcgcg acaatctgga
     3541 gatcagcccc aatttatggg cgggcgttgg cttagtgcgc ggcggtgccg ggacggcact
     3601 ggtgggcgat ggtccgacgg tcgctgcgcg aatcaacgaa tacgccgcgc ttggcatcga
     3661 cagctttgtg ctttcgggct acccgcatct ggaagaagcg tatcgggttg gcgagttgct
     3721 gttcccgcat ctggatgtcg ccatcccgga aattccccag ccgcagccgc tgaatccgca
     3781 aggcgaagcg gtggcaaatg attttatccc ccgtaacgtc gcgcaaagct aaggaatacg
     3841 ataatggcaa cgccagtgaa aaagtggtta ttgcgcgttg ccccctggtt tttaccggtg
     3901 ggcatcgtgg cggtgtggca actggcctcc tcagttggct ggctgtcaac gcgtattttg
     3961 ccctcaccgg aaggagtggt gatggcgttc tggacgctct ccgccagcgg cgaattgtgg
     4021 cagcatctgg cgatcagctc ctggcgggcg ctcattggct tttcaattgg cggatcgctg
     4081 gggttgattt tgggactgat tagcgggcta tctcgctggg gagaacgact gctggatacc
     4141 tccattcaga tgttgcgtaa cgtgccgcat ctggcgctga tcccgctggt gattttgtgg
     4201 tttggcattg atgaatccgc aaaaatcttt ctggtggcgc tcggcacgct attccccatt
     4261 tatatcaaca cctggcacgg catccgtaat atcgatcgcg gtctggtgga aatggcgcgt
     4321 agctatggat tatccggcat accgctgttt atccatgtga tcctgcctgg cgccctgccc
     4381 tcaattatgg tcggcgtgcg ttttgctttg ggcctgatgt ggctgacgct gatcgttgcc
     4441 gaaactattt ctgccaattc cggcattggt tatctggcga tgaatgcgcg ggagtttctg
     4501 caaacggacg tggtggtagt cgctattatt ctttacgccc tgctcggcaa acttgctgac
     4561 gtcagcgcgc agctgttaga gcgtctctgg ttgcgctgga acccggctta tcatttgaag
     4621 gaggccacgg tatgaatact gctcgtctga accagggcac accattgttg ctcaatgcag
     4681 taagcaaaca atacgcggaa aatatcgtcc tgaaccaact ggatttacat attccggcag
     4741 gtcagtttgt ggcggtggtg ggccgcagcg gtggtggcaa aagtaccctg ctgcgcctgc
     4801 tggcaggtct ggaaacgcca accgcaggcg acgtgttagc gggcaccaca ccgctggctg
     4861 agattcagga agacacgcga atgatgtttc aggatgcgcg tctgctaccg tggaaatcgg
     4921 tgattgataa cgttgggtta ggccttaaag gccagtggcg cgatgccgcg cgtcgggcgt
     4981 tggctgcagt aggactggag aaccgcgcag gggaatggcc tgccgcactt tctggcgggc
     5041 agaaacagcg tgtggcgctg gcaagagcgt taattcatcg accgggttta ttattgcttg
     5101 atgaaccgct cggggcgctg gacgccctga ctcggctcga gatgcaggat ttgattgtgt
     5161 cactttggca ggagcacggc tttaccgtac tgttggtgac gcatgatgtg agcgaagcgg
     5221 tagcaatggc tgaccgggta ttgttaattg aagagggaaa aattggtctg gatttgacgg
     5281 tggatatccc ccgtccgcgc cggttagggt cggtgcgatt agcggagctg gaagcggaag
     5341 ttttgcaacg ggtgatgcag cgtgggcact ctgaacaacc gattcggcgt catggttaat
     5401 atgccggatg tggcacacaa aaccagcagt ttcaacggat tgtaggcctg ataagacgcg
     5461 tcaagcgtcg catcaggcat ttgggcacgg ctgccggatg cggcgtgaac gccttatcca
     5521 gcctacaaaa ccagcatttt caacggactt gtaggcctga taagacgcgt caagcgtcgc
     5581 atccggcatt ttggctcaaa agtatcaagc cagtgcttta gttatcttct cgtacagatc
     5641 gccagagaga ttttccagcc ctttcaactg ctccagcgcc gcgcgcattt tctcctgacg
     5701 tttggcatcg taacgtttca ggcgaatcag cggctcaatc aggcgtgaag ccacctgcgg
     5761 gttacggctg ttgaggtcgg taagcatttc caccaggaac tggtaaccgc tgccatcttc
     5821 ggcatggaac gctgccgggt tgctgcccgc aaacgcacca atcaacgaac gaatacggtt
     5881 cgggttgctc atggtaaatg agcgatgctg caacaggcca cgcaccgtct ccagcacatt
     5941 cgccgccggg ctggtggctt gcaggataaa ccatttatcc atcaccagac catcctgatg
     6001 ccacttgtcg tcgtactcct gcatcagcgc gtcacggcaa ggcagctgtg cagcaaccgc
     6061 cgcagaaagc gctgccagcg catcggtcat attgtttgct tcatggtatt gcttgctcac
     6121 cagcacgtca gccagatgcg tttcaccaaa agcaaggaag cgcaggcagg cattacgcag
     6181 agtgcgtttt gcaatatctt catgctcaac acggtactcg ctctggtaat tcgcgttgta
     6241 aatagccagt agctcatccg ccagttcagt cgccagagta cgagtgagtg cttcgcgtac
     6301 ttcggcaata gcaatcgggt cgatgatatc gaacagttca gccatttcat tgacagaagg
     6361 cagcgtcagg atttctgccg ccagcgctgg atcaatcttc tcatcgagca gcaccgcgcg
     6421 gaaagcgtca gccacatgca ccggcagaga cagcagctgc ccttgctgat gacgggcgac
     6481 gtttagcttg atgtaggttg ccagcaggct ttgcgccgca tcccaacggg agaaatcatt
     6541 acgcgcatga cgcatcagga aggtcagttg ctgatcgctc cacttatatt ccagtttcac
     6601 tggcgcagag aattcgcaca gcagcgcagg caccggctgg aagtagacat tatcaaaaac
     6661 aaaggtctgt tcagcctggg tgacgttcag caccgaattc accggatgac cgcctttctg
     6721 caacgggatc actttgcctt cgttatcata cagttcgatg gcaaacggaa tatgcagcgg
     6781 ctgtttttct gcctgatccg gcgtagctgg cgtgcgctgg ctgatggtca gggtgtactg
     6841 ctcagtttcc ggattgtagt cgtctttgac agtcacaacc ggcgtgccgg attggctgta
     6901 ccaacggcgg aaatgggaaa gatcgacatt cgacgcatct tccatcgcct gcacaaagtc
     6961 gtcacaggtc gctgcgctac catcatgacg ctcgaaatag agctgcatcc ctttctggaa
     7021 gttttcttcg ccaagcagag tgtggatcat acgaatcact tccgcgccct tctcgtaaac
     7081 ggtcagggtg tagaagttgt tcatttcaat gaccatatcc gggcgaattg ggtgcgccat
     7141 cgggctggcg tcttctgcaa actgcaatcc gcgcatggtg cgcacattat tgatacggtt
     7201 taccgcgcgg gaaccaaggt cagagctgaa ttcctgatcg cggaagacgg ttaaaccttc
     7261 tttcagactg agctggaacc agtcgcgaca ggtgacacgg ttgccggtcc agttatggaa
     7321 atattcatgg ccgataacgc gttcaatatc gaggtaatct ttgtcggtgg cggtgtcggt
     7381 gcgggccagc acatatttgg agttaaagat attcagcccc ttattctcca ttgcgcccat
     7441 attgaagaaa tccaccgcga cgatcatata gatgtcgagg tcatactcca ggccgaagcg
     7501 ttcttcatcc catttcatgg agtttttcag cgaggtcatt gcccacggcg cacgatcaag
     7561 gttgccacga tcgacataca gctccagcgc tacttcgcga ccagaacgcg tggtgaaagt
     7621 gtcgcgcagc acatcaaagt cgcctgccac cagcgcaaac aggtagcacg gtttcgggaa
     7681 cgggtcctgc caccgtaccc aatgacgtcc attttccagt tcaccttgcg caacgcggtt
     7741 accgttggaa agcaggaagg gatatttggt tttatcggca ataattttgg tggtaaaacg
     7801 cgccagcaca tccgggcggt cgagataata cgtaatatgg cggaaacctt cggcttcaca
     7861 ctgggtgcaa agcgcatcgc ctgactgata aagcccttcc agcgcagtat tcgccgccgg
     7921 gctaatttca ttaacgatct taagcgtaaa acgctccggc aaattactga tgaccagtgc
     7981 gccctcttct tctttccagg cggtccacgg ctcatcatta atataaacag aaaccagttt
     8041 gaggtcttcg ccgttgagac gaagcggaac atctgatgca ccatgacgga cagcctggct
     8101 gaccgcggta acgaccgtct tttgcgcgtc caggtcaaag gtcaagtcaa tatcagtaat
     8161 ctggtaatcc ggcgcacgat aatcgtgacg gtatttggct tgtggctgtt gagtcataaa
     8221 taacctttag catcttttat agagtctggt gttcagtcta ttcctgttgc gtaaatcgcg
     8281 ctatgcagaa tcttcatctt ttcaggtaca aacgccttta ttgctacatt tttataacat
     8341 acaccgcgta atgccatcga ccagaaaggt ggcatatggt gtgatcgggg ttcaataaat
     8401 tgcgaaacaa ggtatactcc agcagttcct gaagatgttt attgtactaa acgctcctgt
     8461 acgaggacgc tactgcgcac ctatgacaca attcgcttct cctgttctgc actcgttgct
     8521 ggatacagat gcttataagt tgcatatgca gcaagccgtg tttcatcact attacgatgt
     8581 gcatgtcgcg gcggagtttc gttgccgagg tgacgatctg ctgggtattt atgccgatgc
     8641 tattcgtgaa caggttcagg cgatgcagta cctgcgcctg caggatgatg aatatcagtg
     8701 gctctctgcc ctgcctttct ttaaggccga ctatcttaac tggctacgcg agttccgctt
     8761 taacccggaa caagtcaccg tgtccaacga taatggcaag ctggatattc gtttaagcgg
     8821 cccgtggcgt gaagtcatcc tctgggaagt tcctttgctg gcggttatca gtgaaatggt
     8881 acatcgctat cgctcaccgc aggccgacgt tgcgcaagcc ctcgacacgc tggaaagcaa
     8941 attagccgac ttctcggcat taaccgccgg tcttgatatg tcgcgcttcc atctgatgga
     9001 ttttggcacc cgccgacgtt tttctcgtga agtacaagaa accatcgtga aacgtctgca
     9061 acaggaatcc tggttcgtgg gcaccagcaa ctacgatctg gcgcgtcggc tttccctcac
     9121 gccgatggga acacaggcac acgaatggtt ccaggcgcat cagcaaatca gcccggatct
     9181 agccaacagc cagcgagctg cacttgctgc ctggctggaa gagtatcccg accaacttgg
     9241 cattgcgtta accgactgca tcactatgga tgctttcctg cgagatttcg gtgtcgagtt
     9301 cgccagtcgc tatcagggcc tgcgccatga ctctggtgac ccggttgaat ggggtgaaaa
     9361 agccattgca cattatgaaa agctgggaat tgatccacag agtaaaacgc tggttttctc
     9421 tgacaatctg gatttacgca aagcggttga gctataccgc cacttctctt cccgcgtgca
     9481 attaagtttt ggtattggga ctcgcctgac ctgcgatatc ccccaggtaa aacccctgaa
     9541 tattgtcatt aagttggtag agtgtaacgg taaaccagtg gcgaaacttt ctgacagccc
     9601 aggcaaaact atctgccacg ataaagcgtt tgttcgggcg ctacgcaaag cgttcgacct
     9661 tccgcatatt aaaaaagcca gttaatatca tcagggagct aatcggctcc ctttttttac
     9721 ctttaattcc gaaatctttc gctgcatttg cgaattctgc ttgtctgatt gcagatgcca
     9781 ggtaacatag gtatcccccc atttacgggt ggacaagtgt ttattttttc cgactattaa
     9841 cagagagaat attatgagcg ttgtgcctgt agccgacgta ctccagggcc gcgtagccgt
     9901 tgacagcgaa gtcaccgtgc gcggatgggt acgtacccgc cgagattcaa aagctggcat
     9961 ctccttcctc gccgtttatg acggttcctg ctttgatcct gtacaggctg tcatcaataa
    10021 ttctctgccc aattacaatg aagacgtctt gcgtctgacc accggctgct cggtcattgt
    10081 gacgggtaaa gtcgtggcgt cgccgggcca ggggcaacaa tttgaaattc aggccagcaa
    10141 ggttgaagtt gctggttggg ttgaagatcc agacacttac ccgatggcgg caaaacgcca
    10201 cagcattgag tatctgcgtg aagtcgctca cctgcgtccg cgcacaaacc tgattggtgc
    10261 cgtcgcgcgc gttcgccata cgctggcgca ggcgctgcat cgcttcttta acgagcaggg
    10321 atttttctgg gtttcaacgc cactgattac cgcatctgat accgaaggtg caggcgaaat
    10381 gttccgtgtt tctacgctgg atctggaaaa tctgccgcgt aacgatcagg gcaaagtgga
    10441 tttcgacaaa gacttctttg gtaaagagtc tttcctgacc gtatctggcc agttgaacgg
    10501 cgaaacctac gcttgcgcat tgtccaaaat ttataccttc ggcccgactt tccgtgctga
    10561 aaactccaac accagccgtc acctggcgga attctggatg ctggagccgg aagtggcgtt
    10621 tgctaacctg aacgatatcg cgggtctggc tgaagccatg ctgaaatatg tcttcaaagc
    10681 ggttctcgaa gaacgcgctg acgacatgaa attcttcgct gaacgcgtag ataaagatgc
    10741 cgtttcacgt ctggaacgct tcattgaggc cgatttcgcg caggtggatt acaccgacgc
    10801 agtgaccatt ctcgaaaact gcggcaggaa gtttgaaaac ccggtttact ggggcgtcga
    10861 tctctcttct gagcatgagc gttatctggc ggaagaacac tttaaagcac cggtagtggt
    10921 taaaaactat ccgaaagata ttaaagcgtt ctatatgcgc cttaacgaag acggtaaaac
    10981 cgttgcggct atggacgttc tggctccggg catcggtgag atcattggtg gctcccagcg
    11041 tgaagaacgt ctggacgtgc tggacgagcg tatgctggaa atgggcctga ataaagaaga
    11101 ttactggtgg tatcgcgatc tgcgtcgcta cggtactgtt ccgcattcag gtttcggtct
    11161 tggttttgaa cgcctgattg cttacgtaac tggtgtgcaa aacgtgcgtg atgtgattcc
    11221 gttcccacgt actccgcgta acgccagctt ctaatttagc gtcttcaaga gccagcaccc
    11281 gctggctctt tttttatcct ttctgtcaag ttatctgttt gttaagtcaa gcaatctatt
    11341 tgcaaccccg ccataacgat catcctgtta cggaatatta cattgcaaca tttacgcaca
    11401 aaaaataacc cgcattctta ttgcggatta gttttttctt agctgatagc acaattttca
    11461 tactattttt tggcattctg gatgtctgaa ag
//
LOCUS       DAZKGF010000156        11450 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZKGF010000156 DAZKGF010000000
VERSION     DAZKGF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.156"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..423
                     /locus_tag="ACHOQV_003980"
     CDS             <1..423
                     /locus_tag="ACHOQV_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFP9632266.1"
                     /translation="PGLQNAEQRTERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASL
                     NDEQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFR
                     ALAAELAAECHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            438..1295
                     /gene="menB"
                     /locus_tag="ACHOQV_003981"
     CDS             438..1295
                     /gene="menB"
                     /locus_tag="ACHOQV_003981"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFP9632267.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1295..2257
                     /gene="menC"
                     /locus_tag="ACHOQV_003982"
     CDS             1295..2257
                     /gene="menC"
                     /locus_tag="ACHOQV_003982"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFP9632268.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSGLLAWVNNWLAGDSELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSLLPLVDVDALERLL"
     gene            2254..3609
                     /gene="menE"
                     /locus_tag="ACHOQV_003983"
     CDS             2254..3609
                     /gene="menE"
                     /locus_tag="ACHOQV_003983"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFP9632269.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AMQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMVEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLDEWVKDKLARFQQPVRWLNLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3719..3985
                     /gene="pmrD"
                     /locus_tag="ACHOQV_003984"
     CDS             3719..3985
                     /gene="pmrD"
                     /locus_tag="ACHOQV_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFP9632270.1"
                     /translation="MEWLVKKSCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(3979..4365)
                     /gene="arnF"
                     /locus_tag="ACHOQV_003985"
     CDS             complement(3979..4365)
                     /gene="arnF"
                     /locus_tag="ACHOQV_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFP9632271.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4365..4700)
                     /gene="arnE"
                     /locus_tag="ACHOQV_003986"
     CDS             complement(4365..4700)
                     /gene="arnE"
                     /locus_tag="ACHOQV_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFP9632272.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4697..6349)
                     /gene="arnT"
                     /locus_tag="ACHOQV_003987"
     CDS             complement(4697..6349)
                     /gene="arnT"
                     /locus_tag="ACHOQV_003987"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFP9632273.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLAAVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFAMDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGAMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRNEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6349..7239)
                     /gene="arnD"
                     /locus_tag="ACHOQV_003988"
     CDS             complement(6349..7239)
                     /gene="arnD"
                     /locus_tag="ACHOQV_003988"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFP9632274.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGMLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7236..9218)
                     /gene="arnA"
                     /locus_tag="ACHOQV_003989"
     CDS             complement(7236..9218)
                     /gene="arnA"
                     /locus_tag="ACHOQV_003989"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFP9632275.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPTIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LTVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9218..10186)
                     /gene="arnC"
                     /locus_tag="ACHOQV_003990"
     CDS             complement(9218..10186)
                     /gene="arnC"
                     /locus_tag="ACHOQV_003990"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFP9632276.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10190..11329)
                     /gene="arnB"
                     /locus_tag="ACHOQV_003991"
     CDS             complement(10190..11329)
                     /gene="arnB"
                     /locus_tag="ACHOQV_003991"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFP9632277.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKSTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWSHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
BASE COUNT         2793 a         2975 c         3069 g         2613 t
ORIGIN      
        1 cctgggttgc aaaatgctga acaacgcacg gaacgtcagc gttccgatcg ccaatgggcg
       61 cagcgttttc gcacagaacc gttaacggcg gtatttgctg actggtatca acagcctgtt
      121 tttgcctcac tcaatgacga acaacgccgg gagctggttg cgctgcgcag caacaataat
      181 ggcgcaacac ttgccgccat gctggaggcg acttctctcg ccgtccagcc cgatttacgt
      241 gctaacctta gcgcccgcac atttgcgttt tattatttat gtggtgaacg tgacagcaaa
      301 ttccgcgccc tggcggcgga actggctgcc gaatgccatg tcattcctcg cgccggacat
      361 aacgcgcacc gggaaaatcc cgctggcgta atcgcaagtc tggcgcagat cttgcgtttc
      421 tgactaaagg acacaatatg atttatcctg atgaagcaat gctttacgca ccggttgaat
      481 ggcacgactg ctccgaaggt tttgaggaca ttcgttatga aaaatccacc gacggtatcg
      541 caaaaatcac cattaatcgt ccgcaggtgc gcaatgcctt ccgtcctctg acggtaaaag
      601 agatgatcca ggcactggca gatgcgcgtt atgacgacaa catcggcgtg atcattctga
      661 ctggcgcagg cgataaagcg ttctgctccg gcggtgacca gaaagtgcgt ggtgattacg
      721 gcggctataa ggatgattcc ggcgtacatc acctgaacgt gctggacttc cagcgtcaga
      781 tccgtacctg cccgaaaccg gttgtcgcga tggtggctgg ctactccatt ggtggcggtc
      841 acgttctgca catgatgtgc gacctgacta tcgcggcaga taatgccatc ttcggtcaga
      901 ctggcccgaa agtcggttcc ttcgacggcg gctggggcgc ttcctacatg gcgcgcatcg
      961 tcgggcagaa aaaagcgcgt gaaatctggt tcctgtgccg tcagtacgac gcgaaacagg
     1021 cgctggatat gggccttgta aataccgtgg tgccgctggc ggatctggaa aaagaaaccg
     1081 tgcgctggtg ccgtgaaatg ctgcaaaaca gcccgatggc gctgcgctgc ctgaaagctg
     1141 cactgaacgc cgactgtgac ggtcaggcgg gtctgcagga gctggcgggc aacgccacca
     1201 tgctgttcta catgacggaa gaaggtcagg aaggtcgcaa cgctttcaac cagaaacgtc
     1261 agcctgattt cagcaaattt aaacggaatc cgtaatgcgt agcgcgcagg tataccgctg
     1321 gcagatcccc atggacgcgg gggtggttct gcgcgacagg cggttaaaaa cccgcgacgg
     1381 gctgtatgtt tgcctgcgtg agggcgagcg cgaagggtgg ggggagatct ccccactacc
     1441 gggcttcagc caggaaacct gggaagaggc acaaagcggg ctgcttgcct gggtaaataa
     1501 ctggctggca ggtgatagcg agctaccgca aatgccttcc gtcgcctttg gcgtaagctg
     1561 tgcattggcg gagctggcag acacgctccc gcaggcggcc aattatcgtg cggcaccact
     1621 gtgtaatggc gatccggacg atctgatcct gaaacttgcc gatatgccgg gcgagaaagt
     1681 ggcgaaggtc aaagtgggat tgtacgaagc cgtgcgcgac ggcatggtgg tcaatctgtt
     1741 gctggaggca attccggatc tgcatttgcg tcttgacgcg aaccgcgcct ggacgccgct
     1801 gaaaggccag cagtttgcca aatacgttaa cccggattat cgcgaccgca tcgcgtttct
     1861 cgaagagccg tgcaaaaccc gcgatgattc ccgtgcgttt gcccgtgaaa ccggcattgc
     1921 cattgcctgg gatgaaagcc tgcgcgagcc ggattttgcc tttgtggctg aagagggcgt
     1981 gcgcgcggta gttatcaaac ccacgctcac gggcagcctg gagaaagtac gcgagcaagt
     2041 gcaggcggcg catgcgctgg ggctgacggc ggtgatcagt tcttccattg agtcgagctt
     2101 aggtctaacg caactggcgc ggattgctgc ctggttaacg ccagacacca ttccgggact
     2161 ggacacgctg gatctgatgc aggcgcagca ggttcgtcgc tggccgggga gcctgctgcc
     2221 gttagtggac gttgatgcac tggagcggct gttatgatct tctctgactg gccgtggcgt
     2281 cactggcggc aagtgcgggg agaagccatc gccttacgtc ttaatgacga gcaactcaac
     2341 tggcgcgagc tttgtgctcg cgtcgatgaa ttagcctcca gctttgcgat gcagggtgtg
     2401 gttgagggca gcggcgtgat gttgcgggcg tggaatacgc cgcaaacgct gctggcctgg
     2461 ctggcgttac tgcaatgcgg ggcgcgggtg ctgcccgtga atccacagct gccgcaaccg
     2521 ttacttgagg aattgctccc caatcttacg ctgcaatttg cgctggtgcc ggatggggaa
     2581 aacacgtttc cggcattaac gtcgctgcac attcagcggg ttgaaggcgc acatgccgct
     2641 acgtggcagc cgacgcgtct gtgctcaatg acgttgacct cgggttctac cgggctgccg
     2701 aaagccgctg tccatactta tcaggcccat cttgccagtg ccgaaggggt gttatcgttg
     2761 attccgtttg gcgatcacga tgactggttg ctctctttac cgctgtttca cgtctccggt
     2821 cagggaatta tgtggcgctg gttatacgct ggtgcgcgga tgacggtgcg tgataaacag
     2881 ccattggagc aaatgctggc aggctgtact cacgcttcac tggtgccaac acaactctgg
     2941 cgtttgctgg ttaaccgcag ttccgtttcc ctgaaagcgg tgttacttgg cggcgcggct
     3001 atcccggtcg agttgacgga acaggcgcgc gagcagggga ttcgttgctt ttgcggctat
     3061 ggtctgaccg agtttgcctc cacggtgtgt gcgaaagaag ccgacggtct ggcagacgtt
     3121 ggttcgccgc tgccgggtcg ggaagtgaaa atcgttaatg atgaagtgtg gctgcgggct
     3181 gctagtatgg tagaaggtta ctggcgtaac gggcaactgg tttcactggt taatgacgaa
     3241 ggctggtacg ctacgcgcga tcgcggtgag atgcataatg gcaagctgac cattgtcgga
     3301 cgtttagaca atctattctt cagtggcgga gagggtattc agccggaaga agtcgagcgc
     3361 gtaattgctg cacatcctgc ggttttgcag gtgtttatcg tccccgttgc cgacaaggag
     3421 tttggtcatc gaccggtggc ggtggtggag tatgaccagc aaaccgttga tcttgacgaa
     3481 tgggtgaaag acaagctggc tcgttttcaa caaccggtgc gctggctgaa tctgccgccg
     3541 gagctgaaaa acggcggtat taaaatttca cgtcaggcgc taaaagagtg ggtgcaacgc
     3601 cagcaataat taaccctccg tttcacttct catttctgcc attccattgc cgttgataaa
     3661 gagtttatct gttaatctgt aaattgatgt gaaaactctt agcaaacagg ataatgcaat
     3721 ggaatggctg gtcaaaaaat cgtgctgcaa taaacaagat aacagacatg taattatgct
     3781 ctgcgatgct ggcggcgcga taaaaatgat cgccgaagtg aagagtgact tcgccgtgaa
     3841 agtgggggat ttactctcgc ctttgcagaa tgcgctttac tgtattaatc gtgaaaagct
     3901 gcacacggtg aaagtccttt ctgcaagctg ttatagcccc gatgagtggg aacggcagtg
     3961 taaagcggca gggaaaactc agtaacgttg ttttgttgtg ggcaggaaaa tcagcatcaa
     4021 cccgctcata atacaggcta ctcccagtag tgctttcagc gaaaaagtcc cttcccagcc
     4081 gggaagtacc attgaagcaa tccataccag cacataactc atactgagca aggcataggc
     4141 tttgcttagt gcaagtttat gtagtgtttt ataccaacaa aaaacggaaa gcagataacc
     4201 cagcagcccg agtaacagta tccgggcatc caggccaaaa gctaacaacg ccgcgataaa
     4261 atcccataag tgcgtcatcg gcggcagatg actcgccgca aaacccaggc ttaattgcgc
     4321 agcggaggcg ataatgacgc tgaataatcc ccacatcagg cccattacac ggtactcccg
     4381 aggatcacaa tgccaccaat aatgaacgcc accccacacc agtgacgcgg cgataccggt
     4441 tcgtgccaca gttttactgc agccagcgtc acccagacaa aattcagact taacatcggg
     4501 taggcaatgc ctaccggtac gttctgcaag accagcagcc agagcaccat ggcaagacca
     4561 agacaagcca gcgccagtcc cagccacagc acgatatgtt tgcgccgttt gtttatcgcc
     4621 acaaagcagg ttgcctgttt ctgacacaac tgcccggcaa cgctaagcaa gctggcaaag
     4681 actaatgtta gccagatcat ttgggacgat actgaatcag caccagacgc tcctgacgat
     4741 cgatggcatc tgcgggcgga atggcgagac tgttgatatc ttcattgcgg tcaaccgaaa
     4801 gcacgagtgt aataatcccc tcctgacgat gttgattaag ccagtttgcg aactcatcac
     4861 cgctgacaaa tctccctttc gcatccggat aattaaggcc gtatttcaac tcacctgtct
     4921 ggcgatacat gatgatgtca tcgcgttgca ggctccatgc cagacctgcg gcaacaccga
     4981 cgctatcagt aagaatatag cggcttggct gaagtgattc ttgtgtcatc tcgacaaaaa
     5041 attgcggatg ttttccttcc atgactctgt caggaattga aaatcctacc agcaacgcca
     5101 gccccagcgg gcaaagcgcg gcaaaagacc aggtcttttc gacgtttgtt aaggtgtacc
     5161 agccgaaaaa tgcccatagc gaaaaaatcg accaggcaca aaagacttta tagctctcga
     5221 aggtttgcca caccggcgtg ttcattgctc cccaggggga gaccacaaat gtggcaataa
     5281 tgccagtgac gccaaaagcg atgttaatcc agccattaat ccgcaacgcc agaggattat
     5341 tttttgctgc cagcaaagcg taatgcgcca tcagcattgc cagaggagca aagcaagaaa
     5401 gaatataggt gggcaatttg cctttggcga cggagaaaaa caacagcggc attatcgtcc
     5461 agctcaacaa atagacggtt gcggaatgct tgcggttttt ccagcctgcg tacagtgctc
     5521 cggggagtaa tcccagccac ggcaggctac cggcaatgat gaccggcaag tagtaccaga
     5581 acggagctct atgttgggca tcatccattg caaagcgttg aatatgctca acccagaaaa
     5641 aatagtgcca gaagtcaggc tcccgctgcg ctatcgccag tccccagggg agaaccgtca
     5701 gtacgcaact gatgaccgcc agccagccgt aaataaagag atctttccag cgtttttgcg
     5761 ttgcaaccca tggaagcacg cttaataccg gcacggcaag ggcgagaaaa cctttggtca
     5821 tcacccccat gccgcaagtg attcccagca gtaaaaatcc cgcgcttttg cctttccacg
     5881 tctgtgcctg cattgccagc cagaagctgc acattcccgc caccagccag aaggcaataa
     5941 acggatcgag cacggcataa gtgccaatgg catagacaat aaacaatgag agataaatca
     6001 ctgcggcgag tagagccaga cgtttatcgc gccataagcg cagagtaaac caggtcacca
     6061 gcgcggcagt taacagggtc gcaaagataa cgcctgcccg cacaccaaag ttattcgcgc
     6121 caaatagcca ttgcccaatg ctgttaatcc agtatccggc aatgggtttt tcgaaataac
     6181 gtagccctaa cagatggggc acaatccagt cgccggatgc cagcatttct cgactgattt
     6241 ccgcataacg cgtttcatcg ggttgccata gcagacgcgt gctgatcggt aacaggtaat
     6301 agcaggcaat aaacgcgaag aggccgataa ggtaacgtac cgatttcatc agcgactacc
     6361 cgcaatttgt tggcaaccca gccagccttc acgtccggca atatttccgc gaacaacttg
     6421 tccgagcggc aacatccctg ataacagttc gcttaaaggg caaaatgtca cgccttcctg
     6481 ggctgcccgt ttaaggagat ccacaaaatt atgctgataa gcgcagcctt caacttctgc
     6541 atgaatggta taaaccggcg tgcctttatc tcgcaggatg cgattgagta accaaccgtt
     6601 aaaatcttct gctttcacat cccgaccaat cacttcatcc caggtgggta aggtcaccgg
     6661 aatttgcgca gtaccagggt ttcctgattc gagcagtgga cgaaacggca tcgccccacg
     6721 acaatcgctg ttgtagcgca aatggaacgc ttcttttgct tcgatcacct gctggtcggc
     6781 acgccagcca gcggctgcgg agcaggttac cggttggccg ataatttctt ccagggtgcg
     6841 aaggccgcgt gcgatatcgt cgatcattgt ttgccgatcc caattaccgc tatgggcttg
     6901 ccaggcatgg tgatcccagg cgtgcaggcc gacttcgtga tgtttagccg cttcacgaat
     6961 gatatcggca ttggcatgac caatctcttt acccggccag gccgtacctg ccagtaaaat
     7021 atcccagcca taaagcgatg ccgcgtttga gcgcagcatc ttccacaaaa actgtggctt
     7081 caccagtcgc cagagatggc ggcccatatt gtccgggccg acgctgaaaa aaatgctggc
     7141 ctgaatatta tgcttactca agatttccag cagacgcggc acgccttcac gggtgccacg
     7201 aaaggtatcg acatcaatgc gtaagcctac tttggtcatg atggtttatc cgtaagatca
     7261 acggtgcgca ggaagaaatc cagcgtttcg tcgatggttt cctgcatatc aattttcggt
     7321 tcccagtcca ggcagcggcg ggcattgcgg atgctcggtt tacgatgctc tacgtcctga
     7381 tatcctttgc catagtagct gctactttcg acaacacgaa agcctgcaaa cggcgggaag
     7441 tgatggcgca gcggatgttt ttcgaagctc gccagcagca tctcgcccag ttcctcaatg
     7501 ctcgcttcgt tctcaggatt gccaatgttg ataatttcgc cgtcgcagcg atttcccgca
     7561 ttttcgataa tgcggtacaa cgcctcgata ccatcccgga tatcagtaaa gcagcgtttt
     7621 tgttttccgc catcaattag cttaattggt gaaccttcta ccagattgag aatgagttgc
     7681 gtaatagcgc gggagctgcc aattcgcgct gcattaaggt tatccagtcg tggtcccatc
     7741 cagttaaacg gccggaagag ggtgaactgt aaaccctctt tttcgccata ggcccagatc
     7801 acccgatcaa gtaattgttt tgacaccgaa taaatccagc gtggtttatt caccgggccg
     7861 acggttaaat tagaatggtc ctcatcgaag tatttatcgc tacacatccc ataaacttct
     7921 gaagttgacg ggaagatgat tcgcttacgg tacttcacgc agtagcggat aatgcgcaga
     7981 ttctcttcaa aatcgagttc aaatacgcgc agcgggttgc gggtatattc aatcggcgtg
     8041 gctatcgcca ccagcggcaa gacgacatca cattttttga cgtgatactc aatccattcg
     8101 gaatgaatac tgatatcgcc ttcgacaaag tgaaaatgcg gatgattcag aaaacggctt
     8161 atcgcatcgc tgccaatatc cagaccgtaa acttcataat gatcttcgcg cagcaggcgt
     8221 tctgtcagat ggttgccaat aaagccattc accccgagga tgagtacccg ggtacggcgt
     8281 cgggcggcac aggcaggctg gctattcaag cgtgaacctt gcaccaggcc cagcgtctgc
     8341 gctaattgcg agccctgcat agtaatgccg tcgcccgcct gtccggtgac gatttccagc
     8401 gcgccatcgc cacaggcaat cagcagtggc gctacagaaa tcacgctccc cggctgtgct
     8461 ttgctggcat gaggatgaac acgcgacgac cagacggtga atttctgatt gccaacatag
     8521 ctgaaggcac ccggccacgg atcggcaacg gcacgtacca tgttgtgcag tacggatgcc
     8581 ggcttatgcc attcgaggaa actgtcatcc ggcgttctgc gaccaaaaca ggtagcttcg
     8641 ttttcgcgct gggcgatttc cagaatatta ccgtgtttaa tggtgggtaa tgtctgctcc
     8701 agtagctggc gcgcggcatg acataactta tgatgcaacg taatggcgat atcgtctggc
     8761 gcaatggcaa tgcgcagttg cgccacaatg gccccggcat cggcacgttt caccattcgg
     8821 tgcaatgtaa cgccagtttc cgtttcaccg ttgaccagca cccagttcag cggcgcgcga
     8881 ccacgatatt ttggtaacag cgaaccatgc agattaaatg cacctgcggg agcgagctgc
     8941 aaaatctcgt cgcaaataag atggcgataa taaaaagaga aaatcacctc tggtgagagt
     9001 tgggcaatac gttccaccca cagcggatga ttaacgttat ctggcgcata aaccggaatg
     9061 cctctttccg ccgccagacg agctaccgaa ccataaaagg ctttttcacc gggattatcg
     9121 gtatgagtaa aaatagcgct aatttcgtaa ccggcagcca gcagggcttc aataccgagg
     9181 catcccatat cgtggtaggc aaaaacgacg gttttcatta ttcattttcc ttgctggatg
     9241 gacggataac ttgctgaaca aaatagcggg ggcgggcgcg gacatcggtg tagatcctgc
     9301 cgatatattc accgagtaat cccatgccga taaactgagc gccaataaaa gtaaacagca
     9361 cggcaaatag cataaagacg ccttctgccg cccattgtgg tccgaaggtc aggcgtaaaa
     9421 tcaccagcag caccgcaatg ctaaaacctc caatcgcaat aatgctgccg agcagactca
     9481 gcatacgtag cggcgttgtg gtaaggcagg tcaccaggtc gtacatcaaa ttaatcaggc
     9541 gcataaaact gtatttggat tcaccaaact cacgctcggc atggtgcact ggaatttcaa
     9601 tggcacggcg ggcgaagata ttcgccagaa tcgggataaa ggtactgcgt tcatggcagt
     9661 gcaacatcgc atcgacaata tgacggcgat aggcgcgcag catacaaccg tagtcaccca
     9721 tcgctttgcc ggtggtgcgc tgaataagcc ggttaatcat cttcgaagcg gttttacgaa
     9781 accagctgtc ctggcggttc tggcgtacag tccctaccac gtcgtaacct tcatcggctt
     9841 ttgccaccag acgggggatt tcctctggcg gattctggag atcggcatcg agggtaataa
     9901 ttaagtcgcc agtaacgtga ctgaatcccg ccataatcgc tgaatgttgc ccgtaattgc
     9961 ggttaagcaa aatagacaca atatggctgt tctccgcttg tgaggcttcg accagcatat
    10021 gcgcggaatt atcgctactg ccgtcatcaa tcagcaggat ttcatactct ttccccagtg
    10081 attcacaggc tgcggtggtg cgcctgatta attccggtaa gctttcctgc tcgttataaa
    10141 cgggaataac caccgagact ttcttaacag ggtggatttc aaacatggct tattgtcctg
    10201 cgagttgctg aagggctgtg atgacgcggt cggcatcggc ggtggtcata tccgggaaca
    10261 gcggcaacga acagatgcgt tcgctattcc attcggtatt cggtaacgac agcgtgggaa
    10321 aacgctcgcg ataatatttt tgtgtgtgag cggcgcggaa atgtaacccg gtaccaatgc
    10381 ctctttcttt taacgcttcc atcaacgcat cgcgactgat accacaacgt tgttcatcga
    10441 cacgaataat aaacagatgc caggcgtgaa cgtgtgacca ggcgggaagg cttaatggct
    10501 gaaaggggag agctgccagt gcttgctgat attgctgggc aatttcgcgc cgacgggtgt
    10561 tgaggtgctc taatttgact aactgtgtca gggcaatcgc ggcgttaata tcggtcagat
    10621 tgtacttata gcccggcgtt aagacttcag cctgcggtgc acggccccag gtatgtctgt
    10681 cataggcatc gacgcccaga ccgtgaaatt ttagcatccg tagctggcgg gcaaggtttt
    10741 cattatctgt aacaatcagg ccaccttccg cacaggtaat atttttgatg gcgtgaaacg
    10801 aaaaaatagc ggtacttttt gcgccaatat gtcgcccttt gtaatacgta cctacggcat
    10861 gggcagcatc ttcgataact gcgatgccgt aacgttcgcc aatggcgcga atggcgtcaa
    10921 tatctgctgg cgcaccggca taatgcaccg gaatgatggc tttagtgcgt ggcgtaatgg
    10981 ctgactcgat cgcttcaggc gtgaccatca gcgtatcgcg gtcgacatcc accattaccg
    11041 gcgttgcacc caacaaggag atcatattga gggttgaaac ccaggtcagg gaaggcgtaa
    11101 tcacttcatc gcccttgcca atttccaacg ccattagcgt gatatgcatt ccggcggtgg
    11161 ctgaactgac cgcgatggca tgctgatttc ccgtcaactg gcaaaaggct tgttcaagcg
    11221 cctgattctt cggaccggtt gtgatccaac cggattcgag aacctcttta actgctgcga
    11281 gttcctccac gcccattgct ggtcgcgaaa aaggcaaaaa ttctgacatt gcttttcctt
    11341 ccgccattga atacctgtcc acttatattt gctataaaga gtgttgtgta tattttgcca
    11401 tttggagcga aattctaagg atagaatatt aacttaacct taaccttaag
//
LOCUS       DAZKGF010000157        11444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZKGF010000157 DAZKGF010000000
VERSION     DAZKGF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.157"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            26..841
                     /gene="gspC"
                     /locus_tag="ACHOQV_003992"
     CDS             26..841
                     /gene="gspC"
                     /locus_tag="ACHOQV_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFP9632278.1"
                     /translation="MPTLRFPFHLANHNKDAAINILIIFISIGLIIFNVNYFHTTIVK
                     NGQIINQPTNAFQSEFSLAALWRNENHAGVKDANPVAVNQETPKLSIALNGIVLTSND
                     ETSFVLINEGSEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPRLPDI
                     IKQPDSESQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNL
                     SLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQRHERTQ"
     gene            825..2777
                     /gene="gspD"
                     /locus_tag="ACHOQV_003993"
     CDS             825..2777
                     /gene="gspD"
                     /locus_tag="ACHOQV_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFP9632279.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNERKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHTPAPSAR"
     gene            2787..4268
                     /gene="gspE"
                     /locus_tag="ACHOQV_003994"
     CDS             2787..4268
                     /gene="gspE"
                     /locus_tag="ACHOQV_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFP9632280.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVISGDTSWDEVMRVASATLENEA"
     gene            4265..5461
                     /gene="gspF"
                     /locus_tag="ACHOQV_003995"
     CDS             4265..5461
                     /gene="gspF"
                     /locus_tag="ACHOQV_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFP9632281.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTAVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQHTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLSNLEIRKRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5471..5908
                     /gene="gspG"
                     /locus_tag="ACHOQV_003996"
     CDS             5471..5908
                     /gene="gspG"
                     /locus_tag="ACHOQV_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFP9632282.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5916..6425
                     /gene="gspH"
                     /locus_tag="ACHOQV_003997"
     CDS             5916..6425
                     /gene="gspH"
                     /locus_tag="ACHOQV_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFP9632283.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAANTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6422..6799
                     /gene="gspI"
                     /locus_tag="ACHOQV_003998"
     CDS             6422..6799
                     /gene="gspI"
                     /locus_tag="ACHOQV_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFP9632284.1"
                     /translation="MNKQSGMTLLEVLLAMSIFATVALALMSSMQGQRNAIERMRNET
                     LALWIADNQLKSQDTFNDENTSSSGKEIINGEEWNWRSDVHSSKDGTLLERTITVTLP
                     NGQKTALTRYQSIHDRSGQAQDD"
     gene            6792..7379
                     /gene="gspJ"
                     /locus_tag="ACHOQV_003999"
     CDS             6792..7379
                     /gene="gspJ"
                     /locus_tag="ACHOQV_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFP9632285.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLGVLAFMVFSQASTLHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPQAPLNEAQTL
                     QTVHWYLRNHTLYRAVRTSVDGRKNQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7372..8355
                     /gene="gspK"
                     /locus_tag="ACHOQV_004000"
     CDS             7372..8355
                     /gene="gspK"
                     /locus_tag="ACHOQV_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFP9632286.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTLDVKDNDRQTTLQQFWPQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8370..9533
                     /gene="gspL"
                     /locus_tag="ACHOQV_004001"
     CDS             8370..9533
                     /gene="gspL"
                     /locus_tag="ACHOQV_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFP9632287.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTPTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMRCYGVAPHGVAATNPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QHQQQETWQRYFPQIKRTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMQKTEKDPVSGVWTVRSSGK"
     gene            9530..9991
                     /gene="gspM"
                     /locus_tag="ACHOQV_004002"
     CDS             9530..9991
                     /gene="gspM"
                     /locus_tag="ACHOQV_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFP9632288.1"
                     /translation="MIKSWWAEKSTSEKQLVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEQITLKHLSFREQP"
     gene            9991..10668
                     /gene="gspO"
                     /locus_tag="ACHOQV_004003"
     CDS             9991..10668
                     /gene="gspO"
                     /locus_tag="ACHOQV_004003"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HFP9632289.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10697..11173)
                     /gene="bfr"
                     /locus_tag="ACHOQV_004004"
     CDS             complement(10697..11173)
                     /gene="bfr"
                     /locus_tag="ACHOQV_004004"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFP9632290.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREES"
     gene            complement(11245..11439)
                     /gene="bfd"
                     /locus_tag="ACHOQV_004005"
     CDS             complement(11245..11439)
                     /gene="bfd"
                     /locus_tag="ACHOQV_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFP9632291.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
BASE COUNT         2988 a         2939 c         2735 g         2782 t
ORIGIN      
        1 aatctatttt attagaacat acatcgtgcc cacactacgc ttccccttcc atctggcgaa
       61 tcataataaa gatgctgcca tcaatatatt gattatattt ataagcatag ggttgattat
      121 ttttaatgtg aattatttcc atacaactat agttaaaaat ggacagatca ttaatcaacc
      181 aacaaacgca tttcagtctg agttcagcct tgccgcttta tggcgtaacg aaaatcatgc
      241 tggggtaaaa gatgcaaatc ccgtggcagt taatcaggaa acacctaagc tctctatagc
      301 gcttaatggc attgtactca catcgaatga tgagacttct tttgtcttaa ttaatgaggg
      361 gagcgaacaa aagcgatact ccctcaacga agcccttgag tcagcgccgg gaacgtttat
      421 tagaaaaata aataaaacca gcgttgtgtt tgaaactcac gggcattacg aaaaagtaac
      481 tctccatcca aggctacctg acatcatcaa gcagcctgat tcagaaagtc aaaatgtact
      541 cgctgattat attatcgcca cgccgatacg tgatggagaa cagatatacg ggcttcgatt
      601 gaaccctcgt aagggcctca atgcatttac caccagcttg ctacaaccag gggatatcgc
      661 cctcaggatc aataatcttt cgctgaccca ccctgatgag gtttcacagg cattaagttt
      721 actgctaact caacaaagtg ctcagtttac aattcgtcgc aacggcgtac cccgcttgat
      781 aaatgtttcc gtcgcggaac ttacaggaat gaatggacag cgtcatgaaa ggactcaata
      841 aaatcacctg ctgcttgctg gcagcactac tcatgccttg tgcagggcac gctgagaacg
      901 aacaatacgg cgctaacttc aataacgccg atatccgcca gttcgtggaa atagtgggtc
      961 agcatcttgg caaaacgatc ctgatcgacc cttcggtaca gggaaccatt tccgtacgca
     1021 gtaatgatac gtttagccaa caggagtact accagttctt tttaagtatt cttgatcttt
     1081 acggttattc cgtgatcacg ctggacaatg gttttctgaa agtggtgcgc tcagctaatg
     1141 taaaaacatc gccagggatg attgctgaca gttctcgtcc aggcgtaggt gatgagttgg
     1201 tcacccgaat cgtaccgctt gagaacgttc ctgctcgtga cctggctccc ctgctccgcc
     1261 agatgatgga tgcgggtagc gtcggtaatg ttgtgcatta tgaaccctcc aacgttctta
     1321 ttctgaccgg tcgtgcctcc accattaata aactgattga agtcataaag cgcgttgatg
     1381 tcatcggcac agagaagcag caaattattc atctggaata tgcgtcagcg gaagatctcg
     1441 ccgaaatttt gaatcaatta atcagcgaaa gccacggtaa aagccagatg cctgccctcc
     1501 tttccgcgaa gattgtggcg gataagcgaa ccaactctct tatcatcagc gggccggaaa
     1561 aagcacgcca gcgcatcact tcattactga aaagccttga tgtagaagag agcgaggaag
     1621 gaaatactcg ggtttattac ctgaaatatg ctaaagccac gaatctggtg gaagtgctaa
     1681 ccggtgtttc cgaaaagctg aaagatgaaa aagggaatga gcgtaagccc tcctcttctg
     1741 gcgcgatgga taacgtcgcc attaccgccg atgaacagac taactctctg gtcattaccg
     1801 ctgaccagtc cgtccaggaa aaactcgcca cggtaattgc gcgtctggac attcgccgtg
     1861 cacaggtgct ggttgaggca atcatcgttg aagttcagga tggaaatgga cttaacctcg
     1921 gcgtgcaatg ggcgaataaa aacgttggag cacagcaatt taccaatacc ggattaccga
     1981 tttttaacgc tgcgcaaggt gtggctgatt ataaaaagaa tggtgggatc accagcgcga
     2041 atcctgcctg ggatatgttt agcgcctaca atggcatggc cgcaggcttt ttcaatggcg
     2101 actggggcgt attgttgact gcgctggcca gtaacaataa aaatgacatc ctcgccaccc
     2161 cgagcatcgt aacgctggat aataaactcg cgtccttcaa cgttggtcag gatgtgccgg
     2221 tgctatctgg ctctcagacc acttctgggg ataacgtctt taatactgtc gaacgcaaaa
     2281 cggtgggcac aaaactcaaa gtcactccgc aggtcaatga aggcgacgcg gtactgctcg
     2341 aaatagagca ggaggtttcc agcgttgact cttcgtctaa ttcgacgctc ggcccgacat
     2401 ttaacacccg aactattcaa aatgccgtgc tggtcaaaac cggtgaaacg gtggtcctgg
     2461 gcggattgct ggatgatttt tctaaagagc aagtgtcaaa ggttcctctg cttggcgata
     2521 ttcctttagt ggggcaactc ttccgctata cttcgaccga gcgcgctaaa cgcaacctga
     2581 tggtatttat ccgcccaacg attatccgtg acgatgatgt ttatcgctca ctgtcaaaag
     2641 agaaatacac ccgctaccgt caggagcaac aacagcgaat cgacgggaaa tcaaaagcgc
     2701 tggttggctc ggaagatttg ccggtgctgg atgagaacac gttcaacagc catacccctg
     2761 cgccctcggc acggtaaggc attcttatga gaattcactc accgtacccc gccagttggg
     2821 cgctggcaca acgaattggt tatctctatt cagagggcga gattatttat ctcgccgata
     2881 cgccattcga acggttactc gatattcaac gtcaggttgg ccagtgccag accatgacca
     2941 gcttgtcaca ggctgatttt gaagcccggc tggaagcggt gttccatcag aataccggtg
     3001 agtcgcaaca gattgcgcag gatatcgatc aatccgtcga tcttctctcg ctttcggaag
     3061 agatgcccgc aaatgaagat ctcctgaatg aagattcagc ggcaccggtt atccgcttga
     3121 tcaatgcgat tttgagtgag gccatcaaag aaaccgcctc cgatatccac attgaaacct
     3181 atgaaaaaac aatgtcgatc cgttttcgca tcgacggtgt tttgcggaca attttacagc
     3241 caaacaaaaa actggcggca ctgcttatct cccgaattaa ggtcatggct cgtcttgata
     3301 tcgccgaaaa acgtattcca caggatggac gtattagttt gcgtatcggg cgacgtaaca
     3361 tagatgtccg cgtatccaca ctgccgtcca tctatggtga acgcgccgtg ctccgtctgc
     3421 tggataaaaa cagcctccag ctttcattga acaacctggg gatgacggca gcggataaac
     3481 aggatttaga aaatctcatc cagcttccac acggtattat cctggtgacc ggaccgacag
     3541 gctccggtaa aagcaccacg ctctacgcca tcctttcggc gctgaatact cccggccgca
     3601 atattctgac ggtagaagat cccgtggaat atgagctgga aggcattggg caaacgcagg
     3661 tgaatacccg tgtggatatg tctttcgctc gcggcctgcg cgccatactt cgccaggacc
     3721 cggatgtcgt catggtgggg gaaattcgtg atacagaaac cgcgcagatt gcggttcagg
     3781 cctcgctcac cggccatctg gtactctcaa cactccacac taacagtgca tcaggcgcag
     3841 tgacccggct ccgcgacatg ggcgtcgaat cattcctgct ttcgtcttcc ctggcaggga
     3901 ttatcgcgca acgtctggtt cgtcgcctgt gtccgcaatg ccgacaattc acgcccgtat
     3961 caccccaaca agcgcagatg tttaaatatc atcagctcgc ggtgacaaca attggcactc
     4021 ccgtaggctg ccctcattgc catcaatccg gctatcaggg gcgcatggcg atccacgaaa
     4081 tgatggtggt gacgccggaa ttacgggccg ctattcatga aaatgtggat gaacaagcac
     4141 tggagcgact ggtccggcaa caacacaatg ccttaatcaa aaatggcctg caaaaagtga
     4201 taagcggtga cacctcctgg gatgaggtta tgcgcgtcgc cagtgccacg ctggagaacg
     4261 aagcatgaat tatcgctatc gcgccatgac ccaggatggt caaaaattgc aggggatcat
     4321 tgatgctaac gatgaacgtc aggcacgact gcggctgcgt gaagaagggc ttttcctgct
     4381 ggatattcgc ccccaaaaaa gttcgggagt aaaaacacgt cgcccgagga tcagccatag
     4441 tgaactgacg cttttcaccc ggcagttggc aaccttaagc gcagcggcat tacctctgga
     4501 agagagcctt gccgtaatcg gtcaacaaag cagtaataaa cgactggctg atgtgttaaa
     4561 tcaggtacgc agcgccatcc ttgaagggca tcccctttcc gatgcgttac aacattttcc
     4621 cacgcttttc gattcgctct accgtaccct ggttaaagcg ggcgaaaaga gcgggctgct
     4681 ggccccggtg ttggaaaaac tggctgatta caatgaaaat cggcagaaaa tccgcagcaa
     4741 actcattcag tcgctgatct acccctgcat gctgactgcg gtggcgattg tggtcgtgat
     4801 tattctcctc actgctgtcg tgcccaaaat taccgaacag ttcgtgcata tgaagcagca
     4861 actgccgctg agtacacgca ttcttttagg tctgagcgac acgttgcaac ataccggccc
     4921 gacattacta gcgacagtgt ttattgtcgc tgtaggtttc tggctctggt taaaacgcgg
     4981 caataaccgc caccgttttc atgccatgtt gctgcgcgtt gcactcatcg gcccgctgat
     5041 ttgcgccatt aacagcgcac gctatctccg cactttaagt attttgcaat ccagcggcgt
     5101 ccctctgctg gatgggatga atttgtccac cgaaagtctc agcaacctcg aaatccgcaa
     5161 gcgtctggca aatgcggcag aaaacgttcg gcagggtaac agcattcatc tttcgctgga
     5221 acaaacggca atcttcccgc cgatgatgct ctacatggtg gcctctggcg aaaaaagcgg
     5281 gcagctcggc acgttaatgg tcagagcagc agataaccag gagacactcc aacaaaatcg
     5341 gatcgcctta acgctctcca tcttcgagcc agcactcatt attacgatgg cactgatcgt
     5401 cctgtttatt gtcgtctcgg tactccaacc tcttcttcaa cttaactcaa tgattaatta
     5461 aggaaagtat atgcgcgcaa cggataagca acgcggtttt acattactgg aaattatggt
     5521 ggtgatcgtc atcattggcg tacttgccag cctggtggtt cccaacctga tgggcaataa
     5581 agaaaaggcg gataaacaaa aagccgtcag cgatattgtg gcgctggaaa acgccctgga
     5641 tatgtacaag ctcgacaatc atcgctatcc aaccacaaat cagggacttg aatccttagt
     5701 cgaagcgccg acactgccac cgctggccgc aaactataac aaggaaggtt atatcaagcg
     5761 tctgcctgcc gatccctggg gcaatgatta tgtcctcgtt aatcctggtg aacatggtgc
     5821 atacgatctg ctttcagcag ggcccgatgg tgaaatggga accgaggacg acatcaccaa
     5881 ctggggtttg agcaagaaga aaaagtaagt cagcgatgaa tcagcaacgc gggtttaccc
     5941 tgctggagat gatgctggtg ctggcgctgg tcgctatcac ggcaagtgtg gtgctcttca
     6001 catatggtcg ggaggatgcc gccaacactc gtgcgcgcga aacagcggcg cggtttaccg
     6061 ctgcacttga attagccatc gaccgcgcta cgctcagtgg tcagcctgtc ggtatccatt
     6121 tctctgattc agcatggcgc attatggtac cgggcaaaac accatcagcc tggcgctggg
     6181 ttccattaca agaagatgcc gcagacgaga gcaaaaatga ctgggatgaa gaactctcaa
     6241 ttcatcttca gccatttaaa cctgatgaca gcaaccaacc gcaggtggtg attctggccg
     6301 acggacaaat cacgcccttc tctttgttga tggctaacgc agggaccggt gagccgctcc
     6361 tgacgttagt ctgttcagga tcatggcccc tggatcaaac gctcgccaga gatacccgcc
     6421 catgaacaaa caatcaggga tgacactgct tgaagtctta ctggcgatga gtattttcgc
     6481 cactgttgcg ctggctttaa tgagcagcat gcaagggcaa cgaaatgcca tcgagcggat
     6541 gcgtaatgag acgctggcgc tgtggatcgc agataaccag cttaagtcgc aagatacatt
     6601 caacgatgaa aacacgtcca gttcaggcaa agagataatt aatggcgaag agtggaactg
     6661 gcgcagcgat gtccattcaa gcaaagacgg cacgcttctg gaacgcacca taacggtgac
     6721 gttacccaac ggtcagaaaa ccgctctgac tcgttatcag agcatccatg atagatccgg
     6781 gcaggcgcaa gatgattaat cgccaacagg gtttcacatt gctggaagtc atggcggcac
     6841 tggcgatctt ctcaatgctt ggcgtgctgg cgtttatggt tttttcgcaa gcgtctacgc
     6901 tacatcaacg ctcacagaaa gaaatccagc agttcaatca actgcaacgc accatcacca
     6961 ttcttgataa cgatctcctg caactggtcg ctcgccgaaa tcggagtacg gataaaatca
     7021 tggtgttggg tgaagaggct atttttacca cgcaaagtcg cgatccgcag gccccgctca
     7081 atgaagcgca aaccttacaa acggttcact ggtatctgcg aaatcatacg ctttaccgtg
     7141 ctgttcgtac ttctgtggat ggcaggaaga atcaacctgc ccaggcgatg ctggagcatg
     7201 tcgagagctt tcttctggaa agtaacagcg gggaaagcca ggagcttcct ctctcagtca
     7261 cgttacatct gaagactcag caatatgggg cgttacagcg ccgttttgca ttacctgagc
     7321 agttagcgag agaagagtcg ccagctcaga cgcaggccgg gaataataac catgaataat
     7381 gaacaacgcg gcgtagcact gctcattgta ctcatgttac tggcgctcat ggctgctctg
     7441 gctgcagaca tgacgatcag ttttcacggc cagcttcatc gaacccgcca ggtaaaccat
     7501 catttgcaac ggcaatatga tattgagctg gcggagaaac ttgcgcttgc cagcctcacc
     7561 ctggacgtta aagataacga cagacaaacc acgttgcagc agttctggcc acaaccgcaa
     7621 cagttgcagc tggaaaatgg caatacagtg aagtggcagt tacgcgatgc ccagcactgt
     7681 tttaatctca atgccttagc aaaaatctct gacgccccgc tggcttcccc tgatttcccc
     7741 gcccaggtat tcagtgccct gctgattaac gccggtatcg atcgcggaaa caccgatgag
     7801 atcgtgcaat caattgctga ctatattgat gtcgatgact caccacgctt tcacggcgca
     7861 gaagataact tttaccaaag ccagacgccg ccccggcata gtgccaatca gatgcttttt
     7921 ctgaccggcg aattgcgtca gataaaaggc ataaccgaaa acatctatca gcggcttatc
     7981 ccgtatgtct gcgttctccc aaccacggaa cttagcatca atcttaatat gcttacggag
     8041 aacgatattc cgctcttcag agcgttattc ctcaataaca tcacggatgc tgacgctcgg
     8101 gtcttattac aaaaaaggcc cagggaaggc tggctaacca cggatgcatt tctttactgg
     8161 gcgcagcagg atttctccgg cgtgaagcct ctggtagctc aggtgaaagg gcatctcttt
     8221 ccctatagcc gctattttac gctgagtacg gaaagtatca gcgacgaaca atcccaggga
     8281 tggcaaagcc atattttctt caaccgtaaa cagcagagcg cccaaatcta tcggcgtacg
     8341 ctgcaactct actaatcata aggatcgtga tgcctgaatc gttgatggtc atacgttctt
     8401 cctcgacgct gcgtaagcat tgggaatgga tgacgttctc cgcagatagc gtatcttcgg
     8461 tacatacgcc cacggacgat cttccgctgg agtcgctggc cgatcagccc ggtgccggaa
     8521 atgttcatct tttgatccca ccagagggcc ttttatatcg ttccctcacg ctgcccaacg
     8581 ccaaatacaa actcaccgca caaacattgc aatggctggc agaggagacg ttacccgaca
     8641 acacccagga ctggcactgg acggttgtcg ataaacaaaa cgaaagtgtc gaggtgatag
     8701 gcattcagtc ggagaaactc agtcgctatc ttgagcgttt acatactgcc ggactcaacg
     8761 tgacgcgggt tttgccagat ggctgttatc ttccctggga agtagatagc tggactctgg
     8821 ttaaccagca aacaagctgg cttattcgca gtgccgcaca tgccttcaat gaactggatg
     8881 aacactggtt acagcatctt gctgcccagt ttccgcctga gaatatgcgt tgttatggtg
     8941 tggcacctca cggcgttgca gcgaccaatc cacttatcca gcatcctgaa ataccatcac
     9001 tgagtttgta ttctgccgat attgcattcc agcgctatga catgctgcat ggtgtttttc
     9061 gtaaacagaa aactgtaagc aaatcgggaa aatggctcgc ccgcctggca gtaagctgtc
     9121 tcgttctcgc cattctttct tttgttggca gccggggcat cgcgctctgg caaacgctta
     9181 agattgaaga tcaacttcag catcaacagc aagaaacctg gcaacgttat ttcccgcaga
     9241 tcaaacgcac ccacaacttt cgtttttact ttaagcagca actcgctcag caatatcctg
     9301 aagctgtgcc gttgctctat catttacaaa cacttctgct ggaacaccct gaactgcagt
     9361 taatggaggc gaactatagc cagaagcaaa agtcgctgac cttaaaaatg agtgctaaga
     9421 gtgaagccaa tatcgatcgt ttctgtgaat taacacagtc ctggcttccg atgcaaaaaa
     9481 cagaaaaaga tccggtcagt ggtgtatgga cagtaaggag ctcagggaaa tgattaaatc
     9541 atggtgggca gaaaaatcca catcggaaaa acagctcgtt gcggcgctgg cggttctctc
     9601 gctcggcgtt ttttgttggc ttggggtgat aaaaccgata gatacctaca ttgcagagca
     9661 tcagtcccac gcgcaaaaaa ttaagaagga catcaaatgg atgcaggatc aagcgagcac
     9721 ccatggttta ctcggtcatc ctgcgcttac gcagcccatc aaaaatatcc ttctcgagga
     9781 agcaaaacgt gagaatttgg ctatcacgct ggaaaacggt ccggacaata ctctgacaat
     9841 tcatcctgtc acagctcccc tcgaaaacgt ctcccgctgg ctcactacgg cacagattac
     9901 atacggtatt gttattgaag accttcagtt tactcttgcc ggaaacgaac agatcaccct
     9961 caagcatttg tccttcaggg agcaaccata atgacaatgc tactgccgct cttcattctg
    10021 gttggtttta ttgcaggtta ttttgttaat gccatggcct atcacctctc gccgttggag
    10081 gataaaacgg cgttaacgtt tcgccaggta ttggttcatt tcaggcaaaa aaaatatgcc
    10141 tggcatgata cagtgcccct gatactttgc gttgctgctg ctatcgcctg cgccctggca
    10201 cctttcacgc ccatcgtgac tggcgcactc tttctctatt tctgtttcgc actcacgctc
    10261 agtgttattg attttcgcac tcagctcctg cccgacaaac tcaccttacc gctgctctgg
    10321 cttggcttgg tatttaatgc gcagtctgga ttaattgatt tacatgatgc ggtttacggc
    10381 gcggtagctg gctatggggt gctgtggtgt gtttactggg gcgtctggtt agtttgtcac
    10441 aaagagggat tgggctacgg tgatttcaag ctactggccg ctgcaggcgc gtggtgtggc
    10501 tggcaaacgt tgccaatgat actgctaatt gcctcactgg gtggcattgg ttacgccatc
    10561 gtttcacaac ttctgcaacg ccggactata accactatcg cattcggccc gtggcttgcg
    10621 ctcggcagca tgataaacct ggggtatctg gcctggatct cgtattaacc gggagggttc
    10681 tccctcccga cacggctcaa ctttcttcgc ggatctgtgc ttgcagataa ttttgcaggc
    10741 ccatcttctg aatcagatca agttccgttt ccagccagtc gatatggcct tcttcatcac
    10801 gcaaaatttc tatcatcata tcgcggctga cgtaatcatg aacgctatcg gcataaccaa
    10861 ttgcctcacg caaattcttc gcgccatcca gctcaagtgc cagatcagaa cgcagcattt
    10921 cctcaacatc ctcaccaatg ttcagtttgc ccaggtcctg taaatttgga agaccttcca
    10981 gaaaaagaat gcgctcaata taacgatcgg cgtgtttcat ctcatcaatg gattcatgat
    11041 actccacatc attgagacgt ttgagacccc agtttttaaa catccgggca tggagaaagt
    11101 actgattgat tgcgacaagc tcatttccca acagtttgtt gagataattt ataaccttag
    11161 tatcaccttt cattttttga ctccttcgct tccactcttt gaagcgtaga acggcttacg
    11221 agtaagtcaa aaaacacact ccgtttatgc ggactcctta aactccggca actgcattaa
    11281 ttcatcctcc atcacttcac gcgcggcccg gacacattta ccgcactgat tgccgaccgg
    11341 aataaatttt tttaattgct ggaacgagtg aggggaaaac tggcgcacag cctgacgaat
    11401 ttttttatcg ctgataccat tacaaagaca aacgtacata gaga
//
LOCUS       DAZKGF010000158        11426 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZKGF010000158 DAZKGF010000000
VERSION     DAZKGF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.158"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            68..2491
                     /gene="pgaA"
                     /locus_tag="ACHOQV_004006"
     CDS             68..2491
                     /gene="pgaA"
                     /locus_tag="ACHOQV_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFP9632292.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYSRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLKS
                     GAPDKANLLAEAYIYKLTGRHQDELRAMTESLPENASKQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELIRLSFMPTRSESERYAIADRALAQYAALEILWHDNSDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKNTRQIIPPWAQYWVASAYLKEQQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMIARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLVELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRQEVSLE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2500..4518
                     /gene="pgaB"
                     /locus_tag="ACHOQV_004007"
     CDS             2500..4518
                     /gene="pgaB"
                     /locus_tag="ACHOQV_004007"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFP9632293.1"
                     /translation="MLRNGNKYILMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVS
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYIYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLTQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            4511..5836
                     /gene="pgaC"
                     /locus_tag="ACHOQV_004008"
     CDS             4511..5836
                     /gene="pgaC"
                     /locus_tag="ACHOQV_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFP9632294.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPRLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5838..6251
                     /gene="pgaD"
                     /locus_tag="ACHOQV_004009"
     CDS             5838..6251
                     /gene="pgaD"
                     /locus_tag="ACHOQV_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFP9632295.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFKKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(6301..7089)
                     /gene="phoH"
                     /locus_tag="ACHOQV_004010"
     CDS             complement(6301..7089)
                     /gene="phoH"
                     /locus_tag="ACHOQV_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFP9632296.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIIRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7715..8986)
                     /gene="efeB"
                     /locus_tag="ACHOQV_004011"
     CDS             complement(7715..8986)
                     /gene="efeB"
                     /locus_tag="ACHOQV_004011"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFP9632297.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGVISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTPESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(8992..10119)
                     /gene="efeO"
                     /locus_tag="ACHOQV_004012"
     CDS             complement(8992..10119)
                     /gene="efeO"
                     /locus_tag="ACHOQV_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFP9632298.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKVNPELLAKVDANFIKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10177..11007)
                     /gene="efeU"
                     /locus_tag="ACHOQV_004013"
     CDS             complement(10177..11007)
                     /gene="efeU"
                     /locus_tag="ACHOQV_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFP9632299.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSVFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3018 a         2819 c         2690 g         2899 t
ORIGIN      
        1 tctttctttt cagttacctg taattagata tagagagaga ttttggcaat acatggagta
       61 atacaggatg tattcaagta gcagaaaaag gtgcccgaaa accaaatggg ctttgaaact
      121 tcttactgcc gcatttttag cagcgagtcc cgcggcgaag agtgctgtta ataacgccta
      181 tgatgcattg attattgaag ctcgcaaagg aaatactcag ccagctttgt catggtttgc
      241 acaaaaatca acactcagta ataaccaaat tgctgactgg ttacaaattg ccttatgggc
      301 cgggcaagat aaacaggtca ttaccgttta cagccgctac cgtcatcagc aattaccagc
      361 gcgtggttat gcagctgtcg ccgtcgctta tcgtaacctg caacaatggc aaaactcgct
      421 tacactgtgg caaaaggcgc tctctctgga gccgcaaaat aaggattatc aacggggaca
      481 aattttaacc ctggcagatg cgggtcacta tgatactgcg ctggttaaac ttaagcagct
      541 taaatctgga gcgccggaca aagccaattt actcgcagaa gcctatatct ataaactgac
      601 ggggcgtcat caggatgaat tacgggcgat gacagagtca ttacctgaaa atgcatctaa
      661 gcaacaatat cccacagaat acgtgcaggc attacgtaat aatcaactag ctgccgcgat
      721 tgacgatgcc aatttaacgc cagatattcg cgctgatatt catgccgaac tgatcagact
      781 gtcgtttatg cctacgcgca gtgaaagtga acgttatgcc attgccgatc gcgccctcgc
      841 ccaatacgct gcattagaaa ttctgtggca cgataactca gaccgcactg cccagtacca
      901 gcgtattcag gttgatcatc ttggcgcgtt attaactcgc gatcgttata aagacgttat
      961 ttctcactat cagcgtttaa aaaatacgag gcaaattatt ccgccctggg cgcaatattg
     1021 ggttgcgtcg gcttatctca aagaacagca gccaaaaaaa gcacagtcaa taatgacaga
     1081 gctcttttat cacaaggaga ccattgcccc ggatttatcc gatgaagaac ttgcggatct
     1141 cttttacagc cacctggaga gtgaaaatta tcccggcgcg ctaactgtca cccaacatac
     1201 cattaatact tcgccgcctt tccttcggtt aatgggcacg cctacgagca tcccgaatga
     1261 tacctggtta caggggcatt cgtttctctc aaccgtagca aaatatagta atgatcttcc
     1321 tcaggctgaa atgatagcca gagagcttgc ttataacgca ccaggaaatc agggactgcg
     1381 cattgattac gcgagtgtgt tacaagcccg cggttggcct cgtgcagcag aaaatgaatt
     1441 aaaaaaagca gaagtgatcg agccacgtaa tattaatctg gaggttgaac aagcatggac
     1501 agcattaacg ttacaagaat ggcagcaggc agctgtctta acgcacgatg ttgtcgaacg
     1561 tgaaccgcaa gatcccggcg ttgtacgttt aaaacgtgcg gttgatgtac ataatcttgt
     1621 agagcttcgt atagctggtt caacaggaat tgatgccgaa ggcccggata gtggtaaaca
     1681 tgatgtcgac ttaaccacca tcgtttattc accaccgctg aaggataact ggcgcggttt
     1741 tgctggattc ggttatgccg atggacaatt tagcgaagga aaagggattg ttcgcgactg
     1801 gctggcgggt gttgagtggc ggtcacgtaa tatctggctc gaggcggagt acgctgaacg
     1861 cgttttcaat catgagcata aacccggcgc gcgcctgtct ggctggtatg attttaatga
     1921 taactggcgt attggttcgc aactggaacg cctctctcac cgcgttccat tacgggcaat
     1981 gaaaaatggt gttacaggca acagtgctca ggcttatgtt cgctggtatc aaaatgagcg
     2041 gcgtaagtac ggtgtctcct gggctttcac tgatttttcc gacagtaacc agcgtcagga
     2101 agtctcactt gagggtcagg aacgcatctg gtcttcacca tatttgattg tcgatttcct
     2161 acccaatctg tattacgaac aaaatacaga acacgacact ccatactaca accctataaa
     2221 aacgttcgat attgttccgg catttgaggc aagtcatttg ttatggcgaa gctatgaaaa
     2281 tagctgggag caaatcttca gcgcaggtgt tggtgcctcc tggcaaaaac attatggcac
     2341 ggatgtcgtc acccaactcg gctacgggca acgtattagc tggaatgacg tgattgatgc
     2401 tggcgcaacg ctacgctggg aaaaacgacc ttatgacggt gacagagaac acaacttata
     2461 cgttgaattc gatatgacat tcagatttta aggataaata tgttacgtaa tggaaataaa
     2521 tatatcctga tgctggtgag tataattatg ctcactgcgt gcattagcca gtcaagaaca
     2581 tcatttatac cgccacagga tcgcgaatct ttactcgccg agcaaccgtg gccgcataat
     2641 ggttttgtgg cgatttcatg gcataacgtt gaagacgaag ctgccgacca gcgttttatg
     2701 tcagtgcgga catcagcact gcgtgaacaa tttgcctggc tgcgcgagaa cggttatcaa
     2761 ccggtcagta ttgctcaaat tcgtgaagca catcgaggag gaaaaccgct accggaaaaa
     2821 gcagtagtgc tgacttttga tgacggctac cagagttttt atacccgcgt cttcccaatt
     2881 cttcaggcct tccagtggcc tgctgtatgg gcccccgtcg gcagttgggt cgatacgcca
     2941 gcggatgaac aagtaaaatt tggcgatgag atggtcagtc gagaatattt tgccacgtgg
     3001 caacaagtgc gagaagttgc gcgttcccgg ctcgttgagg tcgcttctca tacatggaat
     3061 tctcactacg gtattcaggc taatgccacc ggcagcttat tgcctgtata tgtaaatcgt
     3121 gcatatttta ctgaccacgc acggtatgaa accgcagcag aataccggga aagaattcgt
     3181 ctggatgctg taaaaatgac ggaatacctg cgtacaaagg ctgaggtaaa tccacacgtt
     3241 tttgtttggc cttatggcga agcgaatggc atagcaatcg cggaattaaa aaaactcggt
     3301 tatgacatgt tcttcaccct tgaatcaggt ttggcaaatg cgtcgcaatt agattccatt
     3361 ccgcgagtat taatcgccaa taatccctca ttaaaagagt ttgcccagca aattattacc
     3421 gtacaggaaa aaccactaca acgggtaatg catatcgatc ttgattacat ttatgacgaa
     3481 aaccgccagc aaatggatcg caatattgat gtgctaattc agagggtaaa agatatgcaa
     3541 atatctaccg tgtatttaca agcatttgct gatcccgatg gtgatgggct ggttaaagag
     3601 gtctggtttc caaatcgttt gctaccaatg aaagcagata tttttagtcg ggttgcctgg
     3661 caattgcgta cccgctcagg tgtaaacatc tatgcgtgga tgccggtatt aagctgggat
     3721 ttagatccca cattaacgcg agtaaaatac ttaccaactg gggagaaaaa agcacaaatt
     3781 catcctgaac aataccgccg tctctcccct ttcgatgaca gggtcagagc acaagttggc
     3841 atgttatatg atgatcttgc cggacatgct gcttttgatg gcatattgtt ccacgatgat
     3901 gctttgcttt cagattatga agatgccagt gcagcggcta tcgcggctta tcagcaagct
     3961 ggctttagcg ggagtctgag cgaaattcga caaaacccgg agcaattcaa acagtggacc
     4021 cgctttaaaa gtcgtgcgtt aactgacttc actttagaac ttagtgcgcg cgtaaaagcc
     4081 attcgtggtc cacatgttaa aactgcacga aatatttttg cacttccggt aatacaacct
     4141 gaaagtgaag cttggtttgc acagaattat gctgatttcc taaaaagcta tgactggacc
     4201 gctattatgg ctatgcctta tatggaaggc gtcacagaaa aatcggctga tcaatggtta
     4261 acacaattga ccaatcagat gaaaagcatc ccgcaggcta aagacaaatc aattttagaa
     4321 ttacaggcac aaaactggca gaaaaatggt cagcatcagg ctatttcttc acaacaactc
     4381 gctcactgga tgagcctatt acaactgaat ggagtgaaaa attatggtta ttatcccgac
     4441 aattttctgc ataaccaacc tgaaatagac gttattcgtc ctgagttttc aacagcctgg
     4501 tatccgaaaa atgattaatc gcatcgtatc gttttttata ttatgtctgg tgttatgcat
     4561 acctctatgc gtagcgtact ttcactctgg tgaactgatg atgaggttcg ttttcttctg
     4621 gccgtttttt atgtccatta tgtggattat tggcggcgtc tatttctggg tctatcgtga
     4681 acgccactgg ccgtggggag aaaacgcacc agccccccag ttgaaagata atccatctat
     4741 ctccattatc attccctgtt ttaatgagga gaaaaacgtt gaggaaacca tacatgccgc
     4801 tttagcacag cgttatgaga acattgaagt tattgccgta aatgatggtt caacagacaa
     4861 aacccgtgcc atcctggatc gcatggctgc acaaattccc cgtctgcggg tcattcatct
     4921 ggcgcaaaac caggggaaag ccattgcgct taaaaccgga gctgccgcgg cgaaaagtga
     4981 atatctggtg tgcattgatg gcgacgcgtt attagaccgc gatgcagcgg catatattgt
     5041 ggaaccgatg ttgtacaacc cacgtgtggg tgccgtaacc ggtaatcctc gtattcgcac
     5101 acgttctacc ctagtgggta aaattcaggt tggcgagtat tcctcaatta ttggtttgat
     5161 caagcggacc cagcgtatct atggaaatgt atttaccgtt tccggtgtta ttgccgcatt
     5221 tcgtcgcagc gccctggcag aagtgggtta ctggagtgac gatatgatca ccgaagatat
     5281 tgatattagc tggaagctgc agttgaatca gtggacgatt ttttacgagc cacgggcgct
     5341 gtgctggata ttaatgcctg aaacgttaaa agggctgtgg aaacagcgcc tgcgctgggc
     5401 tcagggcggt gcagaagtat tcctcaaaaa tatgacaagg ttgtggcgaa aagaaaactt
     5461 tcgaatgtgg ccactgtttt ttgaatactg cctgacgaca atatgggcct ttacctgcct
     5521 ggtcggtttc attatttacg cagtccaact tgcaggtgta ccgttaaata ttgaattgac
     5581 acatatcgca gcgacacata ctgccggaat attattgtgc acgttatgtt tactgcaatt
     5641 tattgtcagt ctggtaattg agaatcgcta tgagcacaat ctggcttcat cgcttttctg
     5701 gattatttgg tttccggtta ttttctggat gctaagcctg gcaacgacat tggtatcatt
     5761 tacacgagtc atgttgatgc ctaaaaaaca acgcgctcgt tgggtaagtc ccgatcgtgg
     5821 gattctgaga ggttaatatg aacaatctaa ttattacgac ccgacaatcg ccagtacgtt
     5881 tactggttga ttatgttgcc acaaccatct tgtggacatt atttgctttg ttcatattct
     5941 tattcgccat tgatctgctg acgggttatt actggcaaag cgaggccaga agccgacttc
     6001 agttctattt tttgctggca gtggcgaatg ccgtcgtgtt aatcgtctgg gcattgtaca
     6061 ataagctgcg ttttaaaaaa cagcagcatc atgcagcata ccaatatacg ccgcaagaat
     6121 atgcagagag cttagcaata cctgatgagc tttatcagca actacaaaaa agccacagga
     6181 tgagcgtaca cttcaccagt cagggacaaa taaaaatggt ggtttcagaa aaaacgctag
     6241 tccgggtata aacacccaaa acaaagcccg gttcgcccgg gctctgcacc gataatacac
     6301 ttaactgtat gcatgtagcg tacgttggca aagtgccgaa cgtacgcaat cctctttacc
     6361 gaatcggatg atcccgacca tttcatcttc ttcgaaacgt tccagcgcgt cacttaatcc
     6421 ggagcgcaca ccgcgaggta aatcgcattg cgtgatatca ccgttgacga taaccgtcac
     6481 gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat tctgcgcctc
     6541 gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga acggcgcgat
     6601 ttcgaccttc cctatttctg gtcgcaggca gtactgcata aaggaagccc ctaagcgccg
     6661 gaccagcacg tcgtaaaccg ggcgaaaata gggagcaaac ttttctgcga tatctccagg
     6721 taaaaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa tcctgtcgac
     6781 atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaggttt ttccgcaccc
     6841 ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat agtgcaattg
     6901 cgcttcattt cgcgcgagga tgggcgaggt atcgcgactg tcgcgggcca taccaatggc
     6961 ttctacgccg ctcatttgca caagcgaggt gaccgattct tcttcacgct gcttatggct
     7021 gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca ctgctttttg
     7081 tcttcccatg gagagcacct tgagttgttt gtattcatca cacgcgccgt tggcagcgcg
     7141 attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg cttttggata
     7201 aaatgccgaa aaacggctac gcacaccgtt tgcggcgtcg gtaacacgag taaagagagg
     7261 aagtgagaaa aatacagagt gacgaagttg ctaccggagg agaaatctcc gcgagtggtg
     7321 gagccttgct taacaaaaca ttgtccagaa caggacgtta ccatccgcga tctccatagt
     7381 gactgactat cactgccggg aacttccgct gctacttaat aagtacaaca gatctcgcat
     7441 tcattgcaac aatatattca cttatattta accataaaac accatttcag tgacattagt
     7501 ttctactgga aagatgacaa agtgatgaca gtgataaaaa agctgtgtgc tttcagcagg
     7561 attcgcagta ctggtaaaag cttgccggat gcggcgtgaa cgccttatcc ggcctaccga
     7621 acccgcaccg attgtaggca tgataagacg cgcaagcgtc gcatcagcgc acgaacgcct
     7681 ggtgcggcgt aaacgcctta tccgcctaaa aacattaaac ccgcaataat gcgcttccga
     7741 gataatcgtt cgcgtccttc acacccggca gcgcaaagaa atagccgcca ccgataggtt
     7801 tgacatattc ctccagcgcc tctccattca accttttttg tactgtcagg aagccctttt
     7861 ccagatcgtg ttggtagcag acaaacagta gccccatatc gagttgcccg gaattggtga
     7921 cgcccaacga ataactgtag ccacgacgca gcatcagact tgactcactc tctggcgtgc
     7981 ggggattcgc cagccggata tggctgtcca gcgcgatcac cttcccctcc ggatcgctgg
     8041 cgtaatcagg cacatcatgc tcatgctgca ttcccagcgg cgcgccggtt tgcttatcac
     8101 gcccaaatat cgtctgctgt tctttcagcg gcgttctgtc ccaaaattcc acccgaaact
     8161 gaatcaaccg caccgcctgg tagctaccac cgattgtcca cgcaggctcc tgctgatctg
     8221 ccgtcaccca caccactttt tgcatcaact tcgcattctg gctatcggga ttggcagtgc
     8281 cgtctttgaa acccagcaaa ttaatcggtg tctctttacc tttactacgc gccgcgtggt
     8341 cggaaataac cccttcccgc ttccagcgca cgctgagcaa atccggcgtg tgtttgatga
     8401 tatcgcgcag cgcatggatc accgtgtcct gggtgttggc gcaaatttgt agcaacacat
     8461 caccatgaca taacgccgca tccagcgagt cgttggggaa acgcgtcatc ttctgcaact
     8521 ttttcggcat ctgtggcgca aggccaaagc gctcatcaaa caatgagtgg cccaccgata
     8581 acgttatggt gagattatcg ggcgcaatgt agccgccaag aatgccggaa tcgagtggtg
     8641 gcaggcgcgg atttggcgtt tctggtgccg ctccgccctg agtcagaaaa gcaaaacgct
     8701 gagtcaacaa gcgaaacaac cgttcaagat cggctttatc gctggcaagc acatcaaacg
     8761 ccaccagcat cattgcagcc tgttgtggcg tcaggatccc tgcctgatgc tcaccataaa
     8821 acggctgttt ctcattgcgt gcatccggtg aaagcgtacc cggcgcactt tgcgtttttt
     8881 gtgcatgagc gaccggacaa cttcccgcca gcgccagcgc accaatccct ttcagtaaac
     8941 gtcggcgtga cggttcattc acgccgtttt catcttcata ctgcataacg cttagtccag
     9001 ccccagcaca ccgcgaagtt gcgccagatc ttccgccagc gcagtaatcg gtcctttcag
     9061 tgcattccgg tcagcatcgg tcagtttgtc gtaattttca aaaccgtctt tagtacggta
     9121 tttcgccaga atggtatcga cctttatgaa gttggcatcg acttttgcca acagttccgg
     9181 attcactttt tgtagttgtg gacgcagcag atcgacaatt ttctgcgagc cttcaacgtt
     9241 agcctggaaa tcccacagat cggtgtggct gtagcgatct tcttcaccgc tgattttgct
     9301 ggccgccact tcctcaatca gtccggctgc accgccgacc acttttgaag gagggaaagc
     9361 cagttcactg atgcgttttt gcaaatcgac cacatcggta taaagctggt cggcatactt
     9421 atccatccct ttggtggtgt tatcgccaaa caatgctttt tccagacggt ggaaaccggt
     9481 gaatttcgga tcggcggctt tttgctcgta atcatcttca cgggcgtcaa tgctgccatc
     9541 cagatcggag aacagttcag caatcggttc aatgcgctca tagtgctggc gcgtcggcgc
     9601 atacagtgct ttcgcttttt cgatatcgcc tgctttaata gcatcggtaa aggctttggt
     9661 gtcggtcacc agctgcgtgg tttccgccat gacatacgct ttatatgcag taattgcacc
     9721 accaagactt aacagcgcat cactttgcgc cgcatccgcc gttgcctcac ctttgacgat
     9781 caacttccct ttcgggtttg tcagcagacc gcaggtcata tcgtattcac caggctgtaa
     9841 atttgccgtc attttctggc taaaaccagg ggcgatattt tcccgctctt ccaccaccat
     9901 cacgcctttg aggatctccc actccagtgc tttctggctg tggttctgaa taatgaactg
     9961 tgttttcccg gcgttaaccg taatggtcat cggttcgcac tgcttatccg tcacggtcac
    10021 tttgacctgc ggcacatcag cggcgttagc cataaaagca gaagaaaaca gcgcagccac
    10081 gctcaactgc aatgcgttac ggcggaagtt aatggtcatg acgagactat ccctttaaag
    10141 tatgttgtaa ctaagcaaga gtttgcgtcg tatttgttac gcggagcgag acgctgtcgc
    10201 ccctgcgcgt ggtggcagag caaatgccac cagcgccggg atgagataaa taaaccagac
    10261 ggcgacttcg ctgacgctcg gcgcttcctg atagccaaaa atcccttcca tcagcgtgcc
    10321 aaacaccgag tgagttgaga gcaccgcact catatcgaag gcgatttcct gaaagtggtt
    10381 ccacaatccg gcttcatgaa atgcgcgaat ggcaccagcc gccagccctg cggcgacgaa
    10441 gagaataaac aggctggtcc atttaaaaaa tgcaccaaga ttgaggcgaa taccgcccca
    10501 gtagagcagg aagccgagca ccacggctgt agcaagaccg agcattgcac ccagcggcgg
    10561 ccagatcccg acatcttgtt gaaacgccgc cagcaggaaa aagaccgact ccagcccttc
    10621 ccttgcaacg gcaaaaaaga ccatcatcac cagcgcccag ccatgatgat ttccacgctg
    10681 caatgcgcta tcgactgcct gctccagttg tactttgacg ttgcgcgaca ctttgcgcat
    10741 ccagaaaacc atccaggtaa ggatcaccac ggcgatcacc gccacgatac cttcaaacag
    10801 ttcctgttct ttttgcggaa attcgccggt ggtttcgtta atgaagatac ccaagcccag
    10861 gcacaacgca gcggcaagca acacgccaat ccacatcaca ccaatccatc ggcctcgctg
    10921 ggtacgctta agatagctgg caatcaaact gacaatcagc gcggcttcaa gtccttcgcg
    10981 caacataatg agaaacggaa caaacatgcc acccaccctt aaacgttttt ctgagtcaat
    11041 ctatgcaaag aaaagtaaat cgcagtgata gcgattatca ttacggtaaa gaaaaataca
    11101 agcagaatgt ttataaaatg atgttggaat gtaaacaaag aaaggtaatg ccaggggata
    11161 taaacgatta ttccaggtac aaagatggga ttgtttacaa cgagccatgt gttcttgcca
    11221 gatgccgcgt aaatgcctta tccagccgac aaaactgtcc agactcaatg catcagagct
    11281 tccatgtaaa actgataagc gggtgtatca agcaatttcg tccatccgcg attatcaaat
    11341 ctaacttttt gcccggaaca taataacagc tcgggtgttg gtggcgtcaa aatcgctgaa
    11401 gcgttccccg aaggccgggg cagccc
//
LOCUS       DAZKGF010000159        11293 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZKGF010000159 DAZKGF010000000
VERSION     DAZKGF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.159"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            227..1798
                     /gene="garD"
                     /locus_tag="ACHOQV_004014"
     CDS             227..1798
                     /gene="garD"
                     /locus_tag="ACHOQV_004014"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFP9632300.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQGIPLENASIVSLQDEKHVGFQSMVEDILQVAERHLHKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAIP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1947..2282
                     /gene="prlF"
                     /locus_tag="ACHOQV_004015"
     CDS             1947..2282
                     /gene="prlF"
                     /locus_tag="ACHOQV_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFP9632301.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2282..2746
                     /gene="yhaV"
                     /locus_tag="ACHOQV_004016"
     CDS             2282..2746
                     /gene="yhaV"
                     /locus_tag="ACHOQV_004016"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFP9632302.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2801..3610)
                     /gene="agaR"
                     /locus_tag="ACHOQV_004017"
     CDS             complement(2801..3610)
                     /gene="agaR"
                     /locus_tag="ACHOQV_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFP9632303.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3859..5139
                     /gene="kbaZ"
                     /locus_tag="ACHOQV_004018"
     CDS             3859..5139
                     /gene="kbaZ"
                     /locus_tag="ACHOQV_004018"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFP9632304.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDGIVAERAARLAKVAEETCL
                     EHFGEADLVYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLSAIWP
                     RIIALVVQPGVEFDHTNVIDYQPVKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5162..5635
                     /gene="agaV"
                     /locus_tag="ACHOQV_004019"
     CDS             5162..5635
                     /gene="agaV"
                     /locus_tag="ACHOQV_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFP9632305.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5646..6425
                     /gene="agaW"
                     /locus_tag="ACHOQV_004020"
     CDS             5646..6425
                     /gene="agaW"
                     /locus_tag="ACHOQV_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFP9632306.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6415..7293
                     /gene="agaE"
                     /locus_tag="ACHOQV_004021"
     CDS             6415..7293
                     /gene="agaE"
                     /locus_tag="ACHOQV_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFP9632307.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7311..7745
                     /gene="agaF"
                     /locus_tag="ACHOQV_004022"
     CDS             7311..7745
                     /gene="agaF"
                     /locus_tag="ACHOQV_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFP9632308.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKLGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7742..8875
                     /gene="nagA"
                     /locus_tag="ACHOQV_004023"
     CDS             7742..8875
                     /gene="nagA"
                     /locus_tag="ACHOQV_004023"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9632309.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAEIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LERIAQRCQRGGPGAQILGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDDVLGSLKPGKRASVVALDSGLHAQQIWIQGQLASF"
     gene            9225..10379
                     /locus_tag="ACHOQV_004024"
     CDS             9225..10379
                     /locus_tag="ACHOQV_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFP9632310.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQSFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10392..11252
                     /gene="kbaY"
                     /locus_tag="ACHOQV_004025"
     CDS             10392..11252
                     /gene="kbaY"
                     /locus_tag="ACHOQV_004025"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFP9632311.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2695 a         2922 c         3001 g         2675 t
ORIGIN      
        1 agtactattt aaatattatt ttgagcatat gcacataagg ttgcgcgcta aagcacagat
       61 ttgcgcttta ccttgccggg cggcactgca atccctgaaa tgattgacat tgatcacatt
      121 tctgcattta aactcctgac attcttattt cacccaatga agttatttat ttttaaatga
      181 gaccaggtcc tcattttaat aacccctggc tggagaatat tgcacaatgg ccaacatcga
      241 aatcagacaa gaaacgccaa ctgcgtttta tataaaagtt cacgacacag ataatgtggc
      301 aattattgtt aatgataatg gcctgaaagc aggaacgcgt tttccggatg ggctggaatt
      361 aattgaacat attccccagg ggcataaagt cgcattgctg gacattccgg ctaatggtga
      421 aattattcgt tatggcgaag tgattggtta cgccgtgcgc gcaatcccac gcggaagctg
      481 gatcgacgaa tcaatggttg tactaccgga agcgccgccg ttacacacgc tgccactggc
      541 aaccaaagtc ccggcaccct taccgccact ggaaggatac acctttgagg gctatcgcaa
      601 tgccgatggc agcgtgggca ccaaaaacct gctcggcatc accaccagcg tccactgtgt
      661 ggcaggcgtg gtggactatg tagtaaaaat cattgaacgc gatctgctgc cgaaataccc
      721 gaacgtcgat ggcgtggtgg ggctgaatca tctgtacggt tgtggcgtgg cgattaacgc
      781 tccggcggcg gtggtgccca ttcggaccat tcacaacatt tcgcttaacc caaactttgg
      841 cggcgaggtg atggtgattg gcctgggttg cgagaaatta cagccagaac gcctgctggt
      901 cggcacggat gatgtacaag gtattccgct ggagaacgcc agtattgtca gcttgcagga
      961 tgaaaagcat gtcggttttc agtccatggt cgaggatatt ttgcaggtcg ccgaacgcca
     1021 tttgcacaaa ctgaatcaac ggcagcgaga aacctgtcca gcttcagaac tggttgtcgg
     1081 catgcagtgc ggtggcagcg atgcgttttc tggcgtaacg gcaaacccgg cggttgggta
     1141 tgcgtctgat ctactggtgc gctgcggcgc aacggtgatg ttttcagaag tcacggaagt
     1201 gcgtgacgcg atccatctgc tgacaccacg cgcagtgaac gaagaggtcg gcaaacggct
     1261 gctggaggag atggagtggt acgataacta tctcaatatg ggaaaaaccg accgtagcgc
     1321 caacccttcg ccgggcaaca agaaaggcgg tctggcaaat gtggtggaga aagcgctcgg
     1381 ctccattgct aaatcgggta aaagcgcaat tgttgaagtg ctgtcgcccg gtcaacgccc
     1441 gactaaacgc ggattaattt acgccgcgac gccagccagc gattttgtct gtggcacaca
     1501 acaggtggct tcagggatta ccgtgcaagt gtttacgacc ggtcgcggta cgccgtacgg
     1561 cctgatggcg atacccgtca ttaaaatggc gacccgcacc gagctggcga accgctggtt
     1621 tgatttaatg gatattaacg cgggcactat cgctaccggc gaagaaacca ttgaagaagt
     1681 gggctggaag ttgttccact ttattctcga cgtcgccagc gggaagaaga aaactttctc
     1741 ggatcaatgg ggactgcata accagctggc ggtgtttaac ccggcaccag tgacctgatt
     1801 tcttttggaa cgtccctcgc aaaacatggc cttagtgcca tgtttttatt gtttaaagtc
     1861 cacacatcca ttaataatgc atttgcatta ccttagttca agcttataat ttgagcagaa
     1921 aacaggctgt aaaaggacag taaataatgc ccgctaatgc tcgctctaac gctgtactga
     1981 ccactgaatc aaaggtcact atacgcggac aaacaactat ccccgcgcca gtgcgtgagg
     2041 ccttaaaact gaagccaggc ctggacagca ttcattacga aattctgcct ggtgggcaag
     2101 tatttatgtg ccgactggga gatgaacagg aggatcatac tatgaatgca tttttgcgtt
     2161 ttctggatgc agatatccag aacaacccgc aaaaaactcg tccattcgac attcaacaag
     2221 gaaagaaact tgtcgctggc atggacgtca acattgacga tgagattggc gacgacgaat
     2281 aatggatttt ccacaaaggg ttaatggttg ggcgctatat gctcatccct gttttcagga
     2341 aacctacgat gctttagttg ccgaagtcga ggcattaaag ggaaaagatc ctgaaaatta
     2401 tcagagaaaa gctgccacaa agttattggc ggtggtccat aaagtgattg aggagcatat
     2461 cacggtcaat ccatcatcac cggcattccg tcatggcaag tcgttaggct ctgggaaaaa
     2521 taaagactgg tcacgggtaa aatttggtgc tggtcgctat cgtctcttct ttcgttacag
     2581 tgaaaaagag aaagtcatca ttctgggatg gatgaacgat gaaaacacgc tgcgcaccta
     2641 cggtaaaaaa acagatgcct ataccgtatt cagtaaaatg ttaaaaagag gacatcctcc
     2701 tgccgactgg gaaagcctca cccaagagac agaagaaaac cattgatggt gttcacaatg
     2761 cccgccgaac gcattggtgt tgaccagcgg gcatcctttc tcattccccg accagaatca
     2821 cttcaacccc agcctttctc agtccttcca gactatccgc aggaatgcct tcatcaacaa
     2881 tgatcatgtc gatacgttga gtatcaatga tcttatgtaa actagaacga ttgaacttac
     2941 tggaatcggt gaccacgatg atccgttccg caacttcgca catccgacgg tttaaacggg
     3001 cttcatcttc attatgcgtg ctgacgccgc gctccagatc gatcgcatct acaccaagaa
     3061 acagcatatc gaagtggtaa ttttgcagcg attgctcagc ctgatcaccg taaaaagatt
     3121 gcgactgacg gcgcaaatgc cccccggtca tcagcagctc aacgccttcc gcttccagca
     3181 acgcattagc cacgttcata ccgttggtca tcgcaattac gtcggtgtgc ttgcgcatca
     3241 gacgagcaat ctcaaaagtg gtggtcccgg agtcgaggat cacccgatga ccaggctgaa
     3301 tcaactcaac ggcagctttc gcaacgctgc gtttcatcgc ggtgttcagc gcgcttttat
     3361 cttccactga tggctcgact gacggcgttg tgctatcgca gatcaacgcg ccaccatagg
     3421 cacgcacagc gatcccctgc ttttccagaa acgccagatc gttgcggatc gtcacagtag
     3481 atacaccata caacgccgat agatcattaa cttgcacact tccttgctgg cgcaggcgtt
     3541 gaatgatctg ctctcgtcgc tcgctggtgc ctgtcactcg cttctcacct gaagcgtcgg
     3601 tattactcat agtaagtcct ttcgtaaaac tttcgtttca tttcgttttg cctattaacg
     3661 cctttctatt aagcaaatgc aagcccacct tacccattgg cgcaagctac tctcgtttca
     3721 gtggctttca ttatgtttct tttgtgaatc agatcagaaa accattatct ttcgttttat
     3781 ttttatctca ccatgacgca gtatcaactg aaacaaaacg aaagattaat gtcgcagtaa
     3841 tctgaactgg agaggaaagt gaaacatctg acagaaatgg tgagacagca caaagcgggc
     3901 aaaacaaatg ggatttatgc cgtttgttcc gcacatccgc tggtgctgga agctgcaatc
     3961 cgctacgcca gtgcaaacca aacgccgtta ctgattgaag caacctccaa tcaggtggac
     4021 cagttcggcg gttataccgg aatgacgccc gccgattttc gcggctttgt ttgtcagctc
     4081 gccgactcgt tgaatttccc gcaggatgcg ctgattctgg gtggtgacca tctggggcca
     4141 aaccgctggc aaaacctgcc tgccgctcag gcaatggcca atgccgatga tttgattaaa
     4201 agctacgtgg cggcaggatt caaaaaaatt caccttgatt gcagcatgtc ctgtcaggac
     4261 gatccgattc ccttaaccga tggcatcgtg gccgaacgcg ccgcccgtct ggcgaaagtg
     4321 gcggaagaaa cctgtcttga acactttggc gaagccgatc tggtgtatgt cattggcacc
     4381 gaagtaccgg tacctggcgg cgcgcatgaa accttaagcg agctggcggt caccacgccg
     4441 gatgccgccc gcgccacgct ggaagcccat cgtcacgcct ttgaaaagca aggtctgagt
     4501 gccatctggc cacgcatcat tgctctggtg gtacaacccg gcgtcgaatt cgatcacacc
     4561 aacgttattg attatcagcc cgtcaaagcg accgccttaa gccagatggt cgaaaactac
     4621 gaaacgctga ttttcgaagc gcactctacc gattaccaaa cgccgcaatc gttgcgccag
     4681 ctagtgattg accactttgc catcctgaaa gttggcccgg cgctgacctt cgccctgcgt
     4741 gaagctctgt tctctctggc ggcgattgaa gaagaactgg taccagcaaa agcctgttct
     4801 ggtctgcgtc aggtgctgga aaacgtgatg ctcgaccgcc cggaatactg gcaaagccac
     4861 taccacggtg acggcaacgc gcgtcgtctg gcgcgtggtt atagctactc ggatcgcgtg
     4921 cgctattact ggccggacag ccagattgat gacgctttcg ctcatctggt tcgtaatctg
     4981 gcggattcac caattccgct gccgctgatc agtcagtatc tgccgctgca gtacgtgaaa
     5041 gttcgctccg gcgagctgca gccaacgcca cgggaactca ttatcaacca tattcaggac
     5101 atcctggcgc agtaccacac agcctgtgaa ggccaataag caaaacaaag aggaacacgc
     5161 tatgccaaat attgttttaa gtcggattga tgaacgcttg attcacggtc aggtcggcgt
     5221 tcagtgggtc ggatttgcgg gggcaaatct ggtgctggta gccaacgatg aggttgccga
     5281 agatccggta caacaaaacc tgatggaaat ggtactggca gaagggatcg ccgtacgttt
     5341 ctggacgctg caaaaagtca tcgacaacat tcatcgcgcc gccgatcgac agaaaatcct
     5401 gctagtttgt aaaacaccgg ccgatttcct gacgttggtg aaaggtggcg ttccggtgaa
     5461 tcgcattaac gttggcaata tgcactacgc caatggcaag caacaaatcg ccaaaacggt
     5521 ttctgtggat gcgggtgata tcgcagcatt taacgatctg aaagccgctg gggtggaatg
     5581 cttcgttcag ggcgtcccga cagagcctgc tgtagacctc tttaaattac tttgagggat
     5641 tcatcatgga aatcagtctg ttgcaggcat ttgcgttggg aattatcgcc tttatcgctg
     5701 gcctggatat gtttaacggc ttaacccata tgcaccgccc ggtggttctc ggcccgttgg
     5761 tcgggctggt acttggcgat ctgcataccg gtattttaac cggcggtacg ctggaactgg
     5821 tgtggatggg gctggcccct ctggcgggcg cacagccgcc taacgtgatt atcggtacta
     5881 tcgtcggcac ggcgtttgcc attactactg gcgtgaaacc cgatgtcgcg gtcggtgtcg
     5941 ccgtaccttt cgctgtcgca gtacagatgg ggattacctt cctgttctcg gtgatgtccg
     6001 gcgtgatgtc ccgatgcgac cggatggcgg agaacgccga tactcgcggc attgaacgcg
     6061 tgaactatct ggcattgctg gcactcggca cgttctattt tctctgcgct ttcctgccta
     6121 tctacttcgg cgcggaacat gcaaaaacca tcattgatgt cctgccgcaa cgattaattg
     6181 atggcctcgg cgtcgcgggc ggcatcatgc cagcaatcgg ttttgccgtg ctgctgaaaa
     6241 tcatgatgaa aaacgtctac atcccctact tcatcctggg ctttgttgcc gcggcctggc
     6301 tcaagttacc ggtgctggct attgctgccg ctgcgctggc gatggcgctg atcgacctgc
     6361 tgcgtaaatc tcctgaaccg actcaacctg cggcacagaa agaggaattt gaagatggca
     6421 tctaatcaaa ccaccctgcc gaacgtcagc gaaaatgaag aaacactgct gactggcgtc
     6481 aatgaaaacg tgtatgaaga tcagagcatt ggcgcggagc tgacgaaaaa agatatcaac
     6541 cgcgtcgcct ggcgttccat gctgttacag gcttctttca actacgaacg tatgcaggct
     6601 tccggctggc tgtacggcct gctgccagca ctgaaaaaga tccacactaa taaacgcgac
     6661 ctggcgcgcg ccatgaaggg gcatatgggt ttcttcaata cccatccgtt tctggtgacg
     6721 tttgttatcg gcattatcct tgcgatggag cgttctaagc aggacgttaa cagtattcag
     6781 agcaccaaaa ttgccgtcgg tgcgccgctc ggcgggattg gcgatgcgat gttctggcta
     6841 acgctactgc cgatttgtgg cgggataggt gcaagcctcg cgctacaagg ctctattctt
     6901 ggcgctgtcg tctttattgt gctgttcaac gtggtgcacc tggggctgcg ttttggtctg
     6961 gcgcattatg cttaccgcat gggcgtggcg gcgattccac tcattaaagc aaataccaaa
     7021 aaagtcggcc atgcggcatc tatcgttggg atgacggtaa tcggcgcgct ggtggcaacc
     7081 tatgttcgtt taagcaccac gctggaaatc accgcgggcg acgcagtggt taagttacag
     7141 gctgatgtta tcgacaaact gatgccagcc ttcttaccgc tggtctacac cctgaccatg
     7201 ttctggctgg tacgccgcgg ctggagtccg ctgcgcctga ttgcagtgac cgtggttctc
     7261 ggcatcgtcg gtaaattctg ccatttcctt taaatacaat gaggtattag atgttaagta
     7321 ttattttgac agggcatggc gggtttgcca gtggcatgga aaaagcaatg aagcaaatcc
     7381 tcggcgagca atcgcagttt attgccatcg attttccgga aacctcaagc accgcgctgc
     7441 ttacatcgca gcttgaagag gccatcgccc aactggattg tgaagacggc attgtttttc
     7501 tgacggattt gctgggcggc acgccgtttc gcgtagcttc aacgctggcg atgcaaaaac
     7561 tgggctgtga agtgattacc ggcaccaatt tgcaactgct actggagatg gtgctggagc
     7621 gcgaagggtt aagcggtgaa gagtttcgtg tgcaggcgct ggagtgtgga catcgtgggc
     7681 tgaccagtct ggtggacgag ttaggccgct gccatgagga atgtccggtc gaggaaggaa
     7741 tatgacaccc gttttgcgcg ccagaaggct gctgactgaa gagggatggc tcgatgacca
     7801 tcagttgcgt attgctgacg gtgtaatcgc agaaatcgaa ccgattccag tgggcgtgac
     7861 tgaacgcgat gcggaactgc tctgccccgc ttacatcgac acccatgtac acggtggtgc
     7921 gggcgttgat gtaatggatg acgcgccgga tgtgctcgac aagctggcaa tgcacaaagc
     7981 acgcgaaggt gtcggcagtt ggttaccgac caccgtaacc gcgccgctta ataccattca
     8041 tgcggcgctg gaacgtattg ctcaacgttg ccaacgcggc ggacctggtg cacaaatact
     8101 ggggagttat ctcgaaggac cgtacttcac gccgcagaat aaaggcgcgc atccgccgga
     8161 gctgtttcgc gagctggaaa ttgccgagct ggatcaattg attgccgttt ctcagcacac
     8221 cttgcgcgtg gtggcgctgg caccggaaaa agaaggggca ttgcaggcca tccgccatct
     8281 taaacagcaa aacgtaaggg tgatgctggg gcatagcgcg gcgacctggc aacaaacccg
     8341 cgccgcgttt gatgctggtg ccgacggcct ggtgcattgc tataacggga tgacaggttt
     8401 acatcaccgc gaaccgggaa tggttggcgc gggattaacg gacaagcgcg cctggctgga
     8461 actgatagcc gatggtcatc atgtgcatcc ggcggcgatg tcgctgtgtt gttgctgtgc
     8521 gaaagagaga atcgtgctca tcaccgacgc gatgcaggca gccgggatgc cggatggtcg
     8581 ctatacgtta tgtggcgaag aagtgcagat gcacggtggc gttgtccgta ccgcgtccgg
     8641 tggactggcg ggcagtacgc tgtctgtcga tgcggcagtg cgcaacatgg tcgagttgac
     8701 gggcgtaacg cctgcggaag ccatccatat ggcgtcgctg catccggcgc gtatgctggg
     8761 tgttgatgat gttctgggat cgcttaaacc gggcaaacgc gccagcgtcg ttgcgctgga
     8821 tagcgggctg catgcgcaac aaatctggat tcagggtcaa ttagcttcgt tttgatggtt
     8881 tgctccttta ttgggccttc acttcccccg taaggccttt ctttttcttt cgttttgatc
     8941 tgtgcagcgg tgtcggatgc gacgctaatg cgccttatcc gacctacggt tggtgaccgt
     9001 aaggccggat aaagtattta cgccgcatcc agcaatccct tttacttccc ttgtcttttc
     9061 tttcaacgat cacagatttc gttttatttc ctttttctcc attgaacttt cagtttcttt
     9121 tctatagatt ttaaccaacg aaagaaatca ccaagtgaaa tgaaacgaaa ggcaaacgaa
     9181 agcgacaacg ccgacgtcaa gttcatcaga ctaaggattg agttatgcca gaaaattaca
     9241 cccctgctgc tgccgcaacc ggtacatgga ctgaagaaga gatccgccat cagcctcgcg
     9301 catggatccg ttcactcacc aacatcgacg cgctacgttc cgcgctcaat aacttccttg
     9361 aaccgttact gcgcaaagag aatctgcaaa tcatcctgac cggagccgga acatcggcat
     9421 ttatcggtga catcatcgcg ccgtggctcg ccagtcatac cggtaaaaac ttcagcgccg
     9481 taccgaccac cgatctggtc actaatccga tggactacct gaatccagct catccgctgc
     9541 tgttgatctc cttcggtcga tccggcaaca gcccggaaag cgtcgccgcc gtggaactgg
     9601 caaatcaatt tgtacccgag tgctatcacc tgccgatcac ctgcaacgaa gcgggcgctc
     9661 tttaccaaaa cgcgatcaac agcgacaacg cgtttgccct actgatgccc gcagaaacgc
     9721 acgaccgcgg ctttgcgatg accagcagca ttaccaccat gatggccagt tgcctcgcgg
     9781 ttttcgcacc tgagacgatc aacagccaga gcttccgcga cgtggcggat cgttgccagg
     9841 cgatcctgac ctcactgggc gatttcagcg aaggtgtgtt tggttacgca ccgtggaaac
     9901 ggatcgttta tctcggcagc ggtggcttac agggcgcagc acgcgagtcg gcgctgaaag
     9961 tgctggaact gacagcgggt aaactggcgg ccttctatga ttccccgacc ggattccgtc
    10021 atggcccgaa atcgctggtc gataacgaaa cgctggtggt ggtatttgtc tccagccacc
    10081 cttacacccg tcagtatgat cttgatctgc tggcagaact ccgccgcgat aaccaggcaa
    10141 tgcgcgtaat cgccatcgcc gcggaaagca atgacgttat tactgccggt ccacatatca
    10201 tcctgccgcc gtcccgtcac tttatcgacg ttgagcaggc attttgcttc ctgatgtacg
    10261 cccagacatt tgcactgatg cagtcgctgc acatgggtaa tacaccggat accccatcag
    10321 ccagtggcac cgttaaccgc gtggtgcaag gcgtaatcat tcatccgtgg caggcataag
    10381 aggatcgcat tatgagcatt atctccacca aatatctgtt acaggacgcc caggccaatg
    10441 gctacgcggt gcctgccttt aacattcaca acgccgagac gatccaggcg atcctcgaag
    10501 tgtgcagtga aatgcgatca ccggtgatcc tcgccgggac gccggggacc tttaagcata
    10561 tcgcgctgga agagatctac gccctgtgta gcgcctattc cacaacctac aacatgccgc
    10621 tggcactgca tctcgaccac cacgaatcgc tggatgatat tcgccgtaaa gtccacgcag
    10681 gcgtgcgcag cgcgatgatc gacggcagtc acttcccgtt tgccgagaac gtgaagctgg
    10741 tgaaatcggt tgtcgacttc tgccactccc aggattgcag cgtggaagca gaactgggcc
    10801 gcctcggcgg tgttgaagat gacatgagcg ttgacgccga aagtgcattc ctgaccgatc
    10861 cacaagaagc taaacgcttt gtcgaactga ctggcgtcga cagcctggcg gtagcgattg
    10921 gtacggcgca cggcttatac agcaaaacgc cgaagattga tttccagcgg ctggcggaaa
    10981 ttcgtgaagt ggtggatgtt cctctggtgc tgcatggtgc cagcgatgtc ccggatgaat
    11041 ttgtccgtcg cactattgaa cttggcgtca caaaagtgaa cgttgccaca gaattaaaaa
    11101 tagccttcgc tggcgcggtt aaagcctggt ttgcggaaaa tccacagggt aatgatcctc
    11161 gttattatat gcgcgtcggt atggatgcga tgaaagaagt tgtcagaaat aaaattaatg
    11221 tctgtggttc agcgaatcga atttcagcat aatcattagt tttttatgat ttatcccaat
    11281 gtacttcccg att
//
LOCUS       DAZKGF010000160        11266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZKGF010000160 DAZKGF010000000
VERSION     DAZKGF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.160"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1431
                     /locus_tag="ACHOQV_004026"
     CDS             <1..1431
                     /locus_tag="ACHOQV_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFP9632312.1"
                     /translation="NKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIVG
                     LVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAAF
                     VTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSVA
                     VALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISWG
                     GWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLGV
                     FVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGIIG
                     LSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAMLP
                     VFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGAV
                     LTILTFLVHITLGVWWWNMLIGWNML"
     gene            1614..3875
                     /locus_tag="ACHOQV_004027"
     CDS             1614..3875
                     /locus_tag="ACHOQV_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFP9632313.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(4016..5299)
                     /locus_tag="ACHOQV_004028"
     CDS             complement(4016..5299)
                     /locus_tag="ACHOQV_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFP9632314.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5451..5927)
                     /locus_tag="ACHOQV_004029"
     CDS             complement(5451..5927)
                     /locus_tag="ACHOQV_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFP9632315.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNIHFHSLASASITAMFS"
     gene            complement(5986..7275)
                     /gene="bioA"
                     /locus_tag="ACHOQV_004030"
     CDS             complement(5986..7275)
                     /gene="bioA"
                     /locus_tag="ACHOQV_004030"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFP9632316.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHADIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEGQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTVAVNRAVQDETFFCQ"
     gene            7362..8402
                     /gene="bioB"
                     /locus_tag="ACHOQV_004031"
     CDS             7362..8402
                     /gene="bioB"
                     /locus_tag="ACHOQV_004031"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFP9632317.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLASARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            8399..9553
                     /gene="bioF"
                     /locus_tag="ACHOQV_004032"
     CDS             8399..9553
                     /gene="bioF"
                     /locus_tag="ACHOQV_004032"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFP9632318.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSDTVADYLVQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDTRREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            9540..10295
                     /gene="bioC"
                     /locus_tag="ACHOQV_004033"
     CDS             9540..10295
                     /gene="bioC"
                     /locus_tag="ACHOQV_004033"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFP9632319.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSMALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDKIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            10288..10965
                     /gene="bioD"
                     /locus_tag="ACHOQV_004034"
     CDS             10288..10965
                     /gene="bioD"
                     /locus_tag="ACHOQV_004034"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP9632320.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
BASE COUNT         2664 a         2872 c         3086 g         2644 t
ORIGIN      
        1 aataaaaaat cgttatggaa gctaattctg atattagcga tcccatgtat tattggcttt
       61 atgccagctc cggcaggatt aagcgaactg gcgtgggtgc tttttggtat ttacctggcg
      121 gccattgtgg ggctggttat caagcctttc ccggaacctg tcgtactgtt aattgccgtc
      181 gccgcatcga tggtagtggt gggtaactta tcaggtgggg aatttaaaac caccgccgta
      241 ttaagcggtt actcttccgg taccacctgg ctggtgtttt ctgcgtttac tttaagcgcc
      301 gcgtttgtaa ccacaggctt aggtaaacgt attgcctatc tgctgattgg taaaattggt
      361 agcactaccc tgggtctggg ttacgttacg gtatttctcg atctggtact ggctccggca
      421 acaccgtcta acaccgcgcg tgcgggcggc atcgtgttac cgatcatcaa cagcgtggca
      481 gtggctttgg gatcagaacc ggaaaaaagt ccgcgtcgtg ttggacatta cctgatgatg
      541 tccatttaca tggtcactaa aaccaccagc tatatgttct ttaccgcaat ggcggggaac
      601 attctggcgc tgaaaatgat caacgacatt ctgcacctgc aaattagctg gggtggatgg
      661 gcgctggccg ctggattgcc gggtatcatt atgctgctgg tcaccccgct ggtgatttac
      721 accatgtatc cgccagaaat taagaaggtg gataacaaaa ccatcgccaa agcgggcctt
      781 gccgaactgg gaccgatgaa aatccgcgaa aaaatgctgc tcggtgtctt cgtgctggcg
      841 ctgctgggct ggattttcag taagtcactg ggggttgatg aatccaccgt ggcaatcgtt
      901 gttatggcga ctatgctgct gctgggtatc gttacctggg aagacgtggt taaaaataaa
      961 ggcggctgga ataccttaat ctggtacggc ggtattatcg gcttaagctc cttattatcg
     1021 aaagttaaat tctttgaatg gttagctgaa gtctttaaaa ataacctggc atttgatggt
     1081 cacggtaacg ttgctttctt cgttattatt ttcctcagca tcatcgtgcg ttatttcttc
     1141 gcttccggta gtgcctatat cgttgctatg ttacctgtat ttgccatgct ggcgaacgtc
     1201 tccggcgcac cgttaatgtt aaccgcgctg gcactgttgt tctctaactc ctatggcggc
     1261 atggttactc actatggcgg cgcggcaggt ccggtcatct ttggcgtggg ttacaacgat
     1321 attaaatcct ggtggttggt cggtgcggta ctgacgatat taaccttcct ggtgcatatc
     1381 accctcggcg tgtggtggtg gaatatgctg atcggctgga acatgctgta aatatactcg
     1441 tcatacttca agttgcatgt gctgcgtctg cgttcgctca ccccagtcac ttacttatgt
     1501 aagctcctgg ggattcactc gcttgtcgcc ttcctgcaac tcgaattatt tagagtatat
     1561 ccatttatta tctttctgcg cacttcacgg tgcgcagata tctggagcat ttgatgatca
     1621 agttatctga aaaaggcgtg tttctcgcca gcaataacga aataattgcc gaagaacatt
     1681 tcaccggcga aataaaaaaa gaagaagcaa aaaaaggcac tattgcctgg tctattctct
     1741 cttctcataa tacgtccgga aatatggata aacttaaaat taagtttgat tcattagcct
     1801 ctcacgatat tacctttgtt ggtattgtac agaccgctaa agcgtccggt atggaacgtt
     1861 tcccgctgcc gtatgtgctg accaactgcc ataactcact ctgtgctgtg ggcggcacca
     1921 ttaacggtga tgaccatgtt tttggtttat cggcggccca gcgttatggc ggtatttttg
     1981 tgccaccgca tattgcggtc atccatcaat atatgcgtga aatgatggcg ggcggcggca
     2041 aaatgatcct cgggtcagac agccataccc gttacggtgc gttagggaca atggcggttg
     2101 gcgagggcgg cggcgagttg gtaaaacagc tgcttaatga cacctgggat atcgactatc
     2161 cgggcgtggt tgcggtgcat ctgaccggaa aacctgcgcc gtatgtgggg ccgcaggatg
     2221 tggcgctggc tatcatcggt gccgtgttca aaaacggtta cgtcaaaaac aaagtgatgg
     2281 agttcgtagg tcccggtgtt gcagcgctct ctaccgattt ccgtaacagc gttgacgtga
     2341 tgaccactga aacgacctgt ttaagttctg tctggcaaac cgatgaagaa gtccataact
     2401 ggctggcgct gcacggtcgc ggccaggatt actgccaact taaccctcaa ccaatggcgt
     2461 actacgatgg ctgcatcagc gttgatttaa gcgccatcaa accaatgatt gcgctgccat
     2521 tccacccgag caacgtgtat gaaatcgaca cgctgaacca gaacctgacc gacattctgc
     2581 gtgagattga aattgagtcc gaacgcgtgg cgcacggtaa agccaaactc tcgctgctgg
     2641 ataaagtgga aaatggtcgc ctgaaagtgc agcaggggat tatcgcgggc tgttctggcg
     2701 gtaactacga aaacgtcatc gcggcggcga atgcgctgcg cggtcaatcc tgcggcaacg
     2761 ataccttctc attggcggtt tacccgtcat cacagccggt gtttatggat ctcgccaaaa
     2821 aaggtgtggt agcagatttg attggcgcag gcgcaatcat cagaaccgcg ttctgcggcc
     2881 catgctttgg tgcgggcgat acgccaatca acaacggttt gagtattcgc cacaccacgc
     2941 gtaacttccc gaaccgcgaa ggctctaagc cagctaatgg gcagatgtca gcggtggcgt
     3001 tgatggacgc tcgttctatc gctgcaactg cggcaaacgg tggctattta acctctgcca
     3061 gcgaactcga ttgctgggac aacgtgccgg agtacgcctt cgatgtaacg ccgtataaaa
     3121 atcgcgtcta tcaaggcttt gtgaaagggg cgacccagca accgctgatc tacggaccga
     3181 acatcaaaga ctggccggaa ttgggcgcgc tgactgacaa tatcgtcctc aaagtgtgct
     3241 cgaagatcct cgacgaagtc accaccaccg acgaactgat tccttccggt gaaacctctt
     3301 cttatcgttc aaatccgatt ggtctggcgg agtttaccct gtcacgccgc gatcccggtt
     3361 atgttggcag aagtaaagcg actgctgagc tggaaaatca gcgtctggcg gggaatgtca
     3421 gcgagctgac agaggtgttt gcgcgcatta agcagattgc tggtcaggag catattgatc
     3481 cgctgcaaac tgaaattggc agcatggtct atgcggtgaa accaggcgat ggttctgcgc
     3541 gtgagcaggc ggcgagctgc cagcgtgtga ttggcggtct ggcgaatatt gccgaggaat
     3601 acgcgactaa acgctaccgt tctaacgtca tcaactgggg gatgttaccg ctgcaaatgg
     3661 cggaagcacc aacgtttgaa gtgggggatt acatttacat ccctggcatt aaagcggcgc
     3721 tggataatcc tggaacgacg tttaaaggtt atgtgatcca tgaagatgca ccggtaacgg
     3781 agattacgct ctatatggaa agtctgactg ctgaagagcg cgagattatc aaggcgggta
     3841 gtttgattaa cttcaataaa aaccgtcaga tgtaaaaagc gccatgtgaa tgtaggtcgc
     3901 atccggcact tattgtcgga tgcgatgctg gcgcatctta tccgacctac aaatcgcatc
     3961 gaaccgtagg ccggataagg cgtttacgcc gcatccggca catagttaac agctcctact
     4021 tcttcgcctc tgcaaccact ttgctaccca cgccgcggtt attgtattcc cacatgcggt
     4081 tgtagttagt gtcattcaga ttgcgctgta cttcgtcgtt atcatcaacg ctgccggtgt
     4141 tacccgcgaa tggacgattg gaaatcaccg catcagccca cggtttggct gtgttaaaac
     4201 cttcgttgat ggcgctatca cggatcacca cctgaccgtt ggtattggca tcaacatcca
     4261 gcgagcggcc cagttgcgcc acgccatcac cggaagcatt gaaacgactg tttacggcga
     4321 ggaaaccgta gtaaatgttg gacagcgtag ccggtgcaaa cacatacgct tcttgctgag
     4381 tacgtgagtt caccacgcgg aattcggtat tatcgaacac cactgcgccg cgaccagaaa
     4441 cgatatccac atccccttca atgtagctgt tggtcaccag cgtacgcggc tgacgattcg
     4501 tttccagacg gttctgcaca ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa
     4561 tgttaacgtt gttaatctgt acctggtcac catcagtacg cagtgccacc gccggatggt
     4621 tacccgcatc tacgctatcg cccagcgtgt tttcgatggt caggttttgc agttgcagtc
     4681 cattgttttg tgaccagaag accgcagagc agagaacgcc gatactgtcg ctgcgtttgc
     4741 tctggcagct atcgtacata taccacgctg gtttacctgg catatatttg ccgcgcgggt
     4801 tgacgtcgtg acgccagtcg gcggggctca tgcccccatc aagggaaagc ccaatcttca
     4861 catcaatcgg tttttcacct gtaccgtaca gagtaattcc acccggagcg gcagggacat
     4921 ataccgttcc ctgatactca ccaggcatca cggcaatata ctggcgcttg ttggtacgct
     4981 tgataattgc cgcatctacc gccgcctgaa tcgtggtatg cgttacacct tgagtacccg
     5041 ccgggccgac aacaaagtca ggttgtgcag gcagggtaat cggggaagga ttccacgctg
     5101 ccgcacctgg tgtcagggat gcaaaatagt gttgagcatc gaaattctgc gcttcttttg
     5161 ccgacagaat cgggcgagaa gaggtaccag gcgcggtttg atcagaagga cgttgatcgg
     5221 gcggtgttga gctacaggcg gtcagcgtca cgccaaaagc caatgccagc gccagacggg
     5281 aaactgaaaa tgtgttcaca ggttgctccg ggctatgaaa tagaaaaatg aatccgttga
     5341 ggcctgcttt tttatactaa ctttagcgaa acgggaaggt aaaaagacaa aaagttgttt
     5401 ttaatacctt taagtgatac cagatggctt tgcgccatct ggcagagtaa ttaactgaac
     5461 atcgcggtaa tcgatgcgct tgccagagag tggaaatgga tgttaaaccc gaccatcgcg
     5521 ccgctggcac cttcatcgac atcaatacgt tctacatcca gcgcatgaac ggtaaaaatg
     5581 tagcgatggg tttcgccttt cggcggcgct gcaccatcgt acccggtttt accaaagtcg
     5641 gtacgcgtct gcaaaacgcc gtctggcatt gctaccagac cagagccaaa cccttgcggt
     5701 aatacgcggg tatcagcggg taagttaaca actacccagt gccaccagcc ggagccggtt
     5761 ggcgcatccg ggtcgtagca ggtgacaaca aaacttttcg ttcccgcagg aacatcatcc
     5821 cacgccagat gcggtgaaat attatcgcca tcgtaaccca tgccgttaaa gacatgacga
     5881 tgtggcaact tatcgccatc gcgcagatca ttactgatga gtttcatgaa ccctcctttc
     5941 tagtttgcag aaagtgtagc cagaacccct cacgctgact cccggttatt ggcaaaaaaa
     6001 tgtttcatcc tgcaccgcac ggttaaccgc tacggtcaga cgctgcaact gttgcgggag
     6061 aataatatag ggcggcatca ggtaaatcag tttgccaaaa ggccggatcc agacaccctg
     6121 ttcgacaaag aatttttgca gcgctgccat attcaccgga cgagtggttt cgaccacgcc
     6181 gattgccccc agtacgcgca catcggcaac catttcggcg tcacaggcgg gggcaagttg
     6241 ctcgcgtagc tgcccttcaa tagccgccac ctgatgctgc cagtcgccag attcgagaat
     6301 cgccaggctg gcgtttgctg ctgcgcaggc cagcggattg cccataaaag ttggcccatg
     6361 cataaagcag ccggcttcgc cgttactgat ggtttccgca acctcgcgcg tggtgagcgt
     6421 ggcggaaagg gtcattgtgc cgccggttaa ggctttaccg agacacaaaa tatccggcgc
     6481 gatatctgca tgttcacagg caaacagttt gccggtacga ccaaatccgg tggcgatctc
     6541 gtcagcaatc agcaagatac cttcgcgatc gcacattttg cggattcgtt ttaaccattc
     6601 cggatggtag atgcgcatcc cgcctgcgcc ctggacaatc ggttcaatga tcacggcggc
     6661 gatttcatga cgatgtgccg ccatcaggcg ggcaaagccc accatatcgc gttcatccca
     6721 ttcaccatcc atgcggcttt gcggggcggg agcaaacaag ttttctggca gatagccttt
     6781 ccacaggctg tgcattgagt tgtccggatc gcacacagac atcgcgccaa aggtatcgcc
     6841 atgataacca ttgcggaagg tcagaaaacg ctggcgcgct tcgcctttgg cctgccagta
     6901 ctgcaacgcc attttcatcg ccacttccac cgctacggaa ccggagtccg cgagaaaaac
     6961 gcattccagc ggttgcggcg tcatcgccac cagtttgcgg cacagctcaa tggctggcgc
     7021 atgagtgata ccaccaaaca tcacatgcga catggcatca atttgcgtct tcatcgccgc
     7081 attaagctgc gggtgattgt agccgtggat cgccgcccac caggacgaca taccgtcaac
     7141 caggcgtctg ccgtcagaca aaattagctc gcaaccttcg gcgctcgcca ccggataaac
     7201 cggtagcggg gaggtcatgg atgtgtatgg gtgccagata tggcgttggt caaaggcaag
     7261 atcgtccgtt gtcataatcg acttgtaaac caaattgaaa agatttaggt ttacgagtct
     7321 acaccgaatt aacaacaaaa aacacgtttt ggagaagccc catggctcac cgcccacgct
     7381 ggacattgtc gcaagtcaca gaattatttg aaaaaccgtt gctggatctg ctgtttgaag
     7441 cgcagcaggt gcatcgtcag catttcgatc cacgtcaggt gcaggtcagc actttgctgt
     7501 caattaagac cggagcttgt ccggaagatt gcaaatactg cccgcaaagc tcgcgctaca
     7561 aaaccgggct ggaagccgag cggttgatgg aagttgaaca ggtgctggcg tcggcgcgca
     7621 aagcgaaagc ggcaggatcg acgcgcttct gcatgggcgc ggcgtggaag aatccccacg
     7681 aacgcgatat gccctacctg gaacaaatgg tgcagggagt aaaagcgatg gggctggagg
     7741 cgtgtatgac gctgggtacg ttgagtgaat ctcaggcgca gcgccttgcg aatgccgggc
     7801 tggattacta caaccataac ctcgacacct cgccggagtt ttacggcaat atcatcacca
     7861 cacgtactta tcaggaacgc cttgatacgc tggaaaaagt gcgcgatgcc gggatcaaag
     7921 tctgctcagg tggcattgtg ggtttaggcg aaacggtaaa agatcgtgcc ggattattac
     7981 tgcaactggc aaacctgccg acgccgccgg aaagcgtgcc aatcaacatg ctcgtgaagg
     8041 tgaaaggtac tccactggca gataacgatg atgtcgatgc ctttgatttt attcgcacca
     8101 ttgccgtagc gcggatcatg atgccgacct catacgtgcg cctttctgcc ggacgcgagc
     8161 agatgaatga acagactcag gcgatgtgct ttatggccgg ggctaactcg attttctacg
     8221 gttgcaaact gctaaccacg ccgaatccgg aagaagataa agacctgcaa ctgttccgca
     8281 aactggggct aaatccacag caaactgccg tgctggcagg ggataacgaa caacagcagc
     8341 gtctggagca ggcactgatg accccggaca ctgacgaata ttacaacgcg gcagcactat
     8401 gagctggcag gagaaaatca acgcggcgct cgatgcgcgg cgtgctgccg atgccctgcg
     8461 tcgccgttat ccggtggcgc aaggggccgg acgctggctg gtggcggacg atcgccagta
     8521 tctgaacttt tccagtaacg attatttagg tttaagccat catccgcaaa ttatccgtgc
     8581 ctggaagcag agtgcggagc aatttggcgt cggtagcggc ggctccggtc acgtcagcgg
     8641 ttatagcgtg gcacatcagg cgctggaaga agaactggcc gagtggctgg gctattcgcg
     8701 ggcgctgctg tttatctccg gttttgcggc taatcaggca gtcattgccg cgatgatggc
     8761 gaaagaggac cgtattgttg ccgaccggct tagccatgcc tcattgctgg aggctgcaag
     8821 tttaagcccg tcgccgcttc gccgttttgc tcataacgat gtcactcatc tggcgcgact
     8881 gcttgcttcc ccctgtccgg ggcagcaact ggtagtgaca gaaggtgtgt tcagcatgga
     8941 cggcgatagt gcgccactgg aggaaatcca gcaggtaacg caacagcacg atggctggtt
     9001 gatggtcgat gacgcccacg gcacgggcgt tatcggggag caggggcgcg gcagctgctg
     9061 gctgcaaaag gtaaaaccag aattgctggt ggtgactttt ggcaaaggat ttggcgtcag
     9121 cggggcagcg gtgctttgct ccgatacggt ggcggattat ctggtgcaat ttgcccgcca
     9181 tcttatttac agcaccagta tgccgcccgc tcaggcgcag gcattacgtg catcgctggc
     9241 ggtcattcgc agtgatgagg gtgatacacg gcgcgaaaaa ctggcggcac tcattacgcg
     9301 ttttcgtgcc ggagtacagg atttgccgtt tacgcttgct gattcatgca gcgccatcca
     9361 gccattgatt gttggtgata acagccgtgc gttacaactg gcagaaaaac tgcgccagca
     9421 aggctgctgg gtcacggcga ttcgcccgcc aaccgtacct gctggtactg cgcgactgcg
     9481 cttaacacta acagccgcgc atgaaatgca ggatatcgac cgtctgctgg aggtgctgca
     9541 tggcaacggt taataaacaa gccattgcag cggcatttgg acgggcagcc gcacactatg
     9601 agcaacacgc cgatctacag cgccagagtg ctgacgcctt actggcaatg ctcccacagc
     9661 gtaaatacac ccacgtactg gatgcgggtt gtggaccagg ctggatgagc cgccgctggc
     9721 gagagcgtca cgcgcaggtg actgcgttag atctctctcc gccaatgctt gttcaggcac
     9781 gccagaagga tgccgcagac cattatctgg cgggagatat cgaatccctg ccgttagcga
     9841 ctgcaacgtt cgatcttgca tggagcaatc tcgcggtgca gtggtgcggt aatttatcca
     9901 tggcactccg cgagctgtat cgggtggtgc gccccggagg cgtggtcgcg tttaccacgc
     9961 tggtgcaggg atcgttaccc gaactgcatc aggcgtggca ggcggtggac gagcgcccgc
    10021 atgctaatcg ctttttaccg ccagataaaa tcgaacagtc gctaaacgcc gtgcattatc
    10081 aacattatat tcagcccatt acgctgtggt ttgatgatgc gctcagtgcc atgcgttcgc
    10141 tgaaaggcat cggtgccacg catcttcatg aagggcgcga cccgcgaata ttaacgcgtt
    10201 cgcagttgca gcgattgcaa ctagcctggc cgcaacaaca ggggcgatat cctctgacgt
    10261 atcatctttt tttgggagtg attgctcgtg agtaaatgtt attttgtcac cggaacggat
    10321 accgaagtgg ggaaaactgt cgccagctgc gcgcttttac aagccgcaaa ggcagcaggc
    10381 taccggacgg caggttataa accggtggcc tctggcagcg aaaagacccc gaaaggctta
    10441 cgtaatagtg acgcgctggc gttacagcgc aacagtagcc tgcagctgga ttacgcaacg
    10501 gtaaatcctt acaccttcgc agaacccacc tcgccgcata tcatcagcgc gcaagagggc
    10561 aggccgatag aatcattggt aatgagcgcc ggattacgcg cgcttgaaca acaggctgac
    10621 tgggtgttag tggaaggtgc tggcggctgg tttacgccgc tttctgacac tttcactttt
    10681 gcggattggg taacacagga acaactgccg gtgatactgg tagttggtgt gaaactcggc
    10741 tgtattaatc acgcgatgtt gactgcgcag gcaatactac acgccggact gactctggcg
    10801 gggtgggtgg cgaacgatgt cacgcctccg ggaaaacgtc acgctgaata tatgaccacg
    10861 ctcacccgca tgattcccgc gccgctgctg ggcgaaattc cctggcttgc agaaaatcca
    10921 gaaaatgcgg caaccggaaa gtacatcaat cttgccttaa tgtagtcatc ctgtaattgg
    10981 ttaaattgcg agcgagatcg tgtctccgat tgactgcaat ttaaccaatt aaattctaaa
    11041 aaaatcacga aaaaaatttt acctccgcat catgcggtga atgtgggaat tgcccaggcg
    11101 gcgggggata ggggctgcag acagttatcc actattcctg tggataacca tgtgtattag
    11161 agttagaaaa cacgaggcaa gcgagagaat acgcggcttg cacgcgaatt ggcgttaaag
    11221 acggctcaaa gaaatatctt ttatttttta acaggttaga taattg
//
LOCUS       DAZKGF010000161        11077 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZKGF010000161 DAZKGF010000000
VERSION     DAZKGF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.161"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            406..786
                     /gene="atpI"
                     /locus_tag="ACHOQV_004035"
     CDS             406..786
                     /gene="atpI"
                     /locus_tag="ACHOQV_004035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFP9632321.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            795..1610
                     /gene="atpB"
                     /locus_tag="ACHOQV_004036"
     CDS             795..1610
                     /gene="atpB"
                     /locus_tag="ACHOQV_004036"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP9632322.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            1657..1896
                     /gene="atpE"
                     /locus_tag="ACHOQV_004037"
     CDS             1657..1896
                     /gene="atpE"
                     /locus_tag="ACHOQV_004037"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFP9632323.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            1958..2428
                     /gene="atpF"
                     /locus_tag="ACHOQV_004038"
     CDS             1958..2428
                     /gene="atpF"
                     /locus_tag="ACHOQV_004038"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP9632324.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            2443..2976
                     /gene="atpH"
                     /locus_tag="ACHOQV_004039"
     CDS             2443..2976
                     /gene="atpH"
                     /locus_tag="ACHOQV_004039"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP9632325.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            2989..4530
                     /gene="atpA"
                     /locus_tag="ACHOQV_004040"
     CDS             2989..4530
                     /gene="atpA"
                     /locus_tag="ACHOQV_004040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP9632326.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            4581..5444
                     /gene="atpG"
                     /locus_tag="ACHOQV_004041"
     CDS             4581..5444
                     /gene="atpG"
                     /locus_tag="ACHOQV_004041"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP9632327.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            5471..6853
                     /gene="atpD"
                     /locus_tag="ACHOQV_004042"
     CDS             5471..6853
                     /gene="atpD"
                     /locus_tag="ACHOQV_004042"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP9632328.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            6874..7293
                     /locus_tag="ACHOQV_004043"
     CDS             6874..7293
                     /locus_tag="ACHOQV_004043"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP9632329.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            7642..9012
                     /gene="glmU"
                     /locus_tag="ACHOQV_004044"
     CDS             7642..9012
                     /gene="glmU"
                     /locus_tag="ACHOQV_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP9632330.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQSQKEGWR
                     RPVKKK"
     gene            9174..11003
                     /gene="glmS"
                     /locus_tag="ACHOQV_004045"
     CDS             9174..11003
                     /gene="glmS"
                     /locus_tag="ACHOQV_004045"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP9632331.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         2664 a         2734 c         3020 g         2659 t
ORIGIN      
        1 cattattaaa aatgtcaatg ggtggttttt gttgtgtaaa tgtcatttat taaaacagta
       61 tctgttttta gactgaaata tcataaactt gcaaaggcat catttgccaa gtaaataaat
      121 atgctgtgcg cgaacatgct caatatgtga tctgaagcac gctttatcac cagtgtttac
      181 gcgttattta cagtttttca tgatcgaaca gggtttgcag aaaagtcgca attgtatgca
      241 ctggaaaaat atttaaacat ttattcacct tttggctact tattgtttga aatcacgggg
      301 gcgcaccgta taatttgacc gctttttgat gcttgactct aagccttaaa gaaagtttta
      361 tacgacacgc ggcatacctc gaagggagca ggagtgaaaa acgtgatgtc tgtgtcgctc
      421 gtgagtcgaa acgttgctcg gaagcttctg ctcgttcagt tactggtggt gatagcaagt
      481 ggattgctgt tcagcctcaa agaccccttc tggggcgtct ctgcaataag cgggggcctg
      541 gcagtttttc tgcctaacgt tttgtttatg atatttgcct ggcgtcacca ggcgcataca
      601 ccagcgaaag gccgggtggc ctggacattc gcatttggcg aagctttcaa agttctggcg
      661 atgttggtgt tactggtggt ggcgttggcg gttttaaagg cggtattctt gccgctgatc
      721 gttacgtggg ttttggtgct ggtggttcag atactggcac cggctgtaat taacaacaaa
      781 gggtaaaagg catcatggct tcagaaaata tgacgccgca ggattacata ggacaccacc
      841 tgaacaacct tcagctggac ctgcgtacat tctcgctggt ggatccacat aaccccccag
      901 ccaccttctg gacaatcaat attgactcca tgttcttctc ggtggtgctg ggtctgttgt
      961 tcctggtttt attccgtagc gtagccaaaa aggcgaccag cggtgtgcca ggtaagtttc
     1021 agaccgcgat tgagctggtg atcggctttg ttaatggtag cgtgaaagac atgtaccatg
     1081 gcaaaagcaa gctgattgct ccgctggccc tgacgatctt cgtctgggta ttcctgatga
     1141 acttgatgga tttactgcct atcgacctgc tgccgtacat tgctgaacat gtactgggtc
     1201 tgcctgcact gcgtgtggtt ccgtctgcgg acgtgaacgt aacgctgtct atggcactgg
     1261 gtgtatttat cctgattctg ttctacagca tcaaaatgaa aggcatcggc ggcttcacga
     1321 aagagttgac gctgcagccg ttcaatcact gggcgttcat tcctgtcaac ttaatccttg
     1381 aaggggtaag cctgctgtcc aaaccagttt cactcggttt gcgactgttc ggtaacatgt
     1441 atgccggtga gctgattttc attctgattg ctggtctgtt gccgtggtgg tcacagtgga
     1501 ttctgaatgt gccgtgggcc attttccaca tcctgatcat tacgctgcaa gccttcatct
     1561 tcatggttct gacgatcgtc tatctgtcga tggcgtctga agaacattaa tttaccaaca
     1621 ctactacgtt ttaactgaaa caaactggag actgtcatgg aaaacctgaa tatggatctg
     1681 ctgtacatgg ctgccgctgt gatgatgggt ctggcggcaa tcggtgctgc gatcggtatc
     1741 ggcatcctcg ggggtaaatt cctggaaggc gcagcgcgtc aacctgatct gattcctctg
     1801 ctgcgtactc agttctttat cgttatgggt ctggtggatg ctatcccgat gatcgctgta
     1861 ggtctgggtc tgtacgtgat gttcgctgtc gcgtagtaag cgttgctttt atttaaagag
     1921 caatatcaga acgttaacta aatagaggca ttgtgctgtg aatcttaacg caacaatcct
     1981 cggccaggcc atcgcgtttg tcctgttcgt tctgttctgc atgaagtacg tatggccgcc
     2041 attaatggca gccatcgaaa aacgtcaaaa agaaattgct gacggccttg cttccgcaga
     2101 acgagcacat aaggaccttg accttgcaaa ggccagcgcg accgaccagc tgaaaaaagc
     2161 gaaagcggaa gcccaggtaa tcatcgagca ggctaacaaa cgccgctcgc agattctgga
     2221 cgaagcgaaa gctgaggcag aacaggaacg tactaaaatc gtggcccagg cgcaggcgga
     2281 aattgaagcc gagcgtaaac gtgcccgtga agagctgcgt aagcaagttg ctatcctggc
     2341 tgttgctggc gccgagaaga tcatcgaacg ttccgtggat gaagctgcta acagcgacat
     2401 cgtggataaa cttgtcgctg aactgtaagg agggaggggc tgatgtctga atttattacg
     2461 gtagctcgcc cctacgccaa agcagctttt gactttgccg tcgaacacca aagtgtagaa
     2521 cgctggcagg acatgctggc gtttgccgcc gaggtaacca aaaacgaaca aatggcagag
     2581 cttctctctg gcgcgcttgc gccagaaacg ctcgccgagt cgtttatcgc agtttgtggt
     2641 gagcaactgg acgaaaacgg tcagaacctg attcgggtta tggctgaaaa tggtcgtctt
     2701 aacgcgctcc cggatgttct ggagcagttt attcacctgc gtgccgtgag tgaggctacc
     2761 gctgaggtag acgtcatttc cgctgccgca ctgagtgaac aacagctcgc gaaaatttct
     2821 gctgcgatgg aaaaacgtct gtcacgcaaa gttaagctga attgcaaaat cgataagtct
     2881 gtaatggcag gcgttatcat ccgagcgggt gatatggtca ttgatggcag cgtacgcggt
     2941 cgtcttgagc gccttgcaga cgtcttgcag tcttaagggg actggagcat gcaactgaat
     3001 tccaccgaaa tcagcgaact gatcaagcag cgcattgctc agttcaatgt tgtgagtgaa
     3061 gctcacaacg aaggtactat tgtttctgta agtgacggtg ttatccgcat tcacggcctg
     3121 gccgattgta tgcagggtga aatgatctcc ctgccgggta accgttacgc tatcgcactg
     3181 aacctcgagc gcgactctgt aggtgcggtt gttatgggtc cgtacgctga ccttgccgaa
     3241 ggcatgaaag ttaagtgtac tggccgtatc ctggaagttc cggttggccg tggcctgctg
     3301 ggccgtgtgg ttaacactct gggtgcacca atcgacggta aaggtccgct ggatcacgac
     3361 ggcttctctg ctgtagaagc aatcgctccg ggcgttatcg aacgtcagtc cgtagatcag
     3421 ccggtacaga ccggttataa agccgttgac tccatgatcc caatcggtcg tggtcagcgt
     3481 gaattgatca tcggtgaccg tcagaccggt aaaaccgcac tggctatcga tgccatcatc
     3541 aaccagcgcg attccggtat caaatgtatc tatgtcgcta tcggccagaa agcgtccacc
     3601 atttctaacg tggtacgtaa actggaagag cacggcgcac tggctaacac catcgttgtg
     3661 gtagcaaccg cgtctgaatc cgctgcactg caatacctgg caccgtacgc tggttgtgca
     3721 atgggcgaat acttccgtga ccgcggtgaa gatgcgctga tcatttacga tgacctgtct
     3781 aaacaggctg ttgcttaccg tcagatctcc ctgctgctcc gtcgtccgcc aggacgtgaa
     3841 gcattcccgg gcgacgtatt ctacctccac tctcgtctgc tggagcgtgc tgcgcgtgtt
     3901 aacgccgaat acgttgaagc cttcaccaaa ggtgaagtga aagggaaaac cggttctctg
     3961 actgcgctgc cgattatcga aactcaggcg ggtgacgttt ctgcgttcgt tccaaccaac
     4021 gtaatctcca ttaccgatgg tcagatcttc ctggaaacca acctgttcaa cgccggtatt
     4081 cgtcctgcgg ttaacccggg tatttccgta tcccgtgttg gtggtgcagc acagaccaag
     4141 atcatgaaaa aactgtccgg tggtatccgt accgctctgg cacagtatcg tgaactggca
     4201 gcgttctctc agtttgcatc cgaccttgac gatgcaacac gtaagcagct tgaccacggt
     4261 cagaaagtga ccgaactgct gaagcagaaa cagtatgcgc cgatgtctgt tgcgcaacag
     4321 tctctggttc tgttcgcagc agaacgtggt tacctggcgg atgtagaact gtcgaaaatc
     4381 ggcagcttcg aagccgctct gctggcttac gtcgaccgtg atcacgctcc gttgatgcaa
     4441 gagatcaacc agaccggtgg ctacaacgac gaaatcgaag gcaagctgaa aggcatcctc
     4501 gattccttca aagcaaccca atcctggtaa cgtctggcgg cttgccttag ggcaggccgc
     4561 aaggcattga ggagaagctc atggccggcg caaaagagat acgtagtaag atcgcaagcg
     4621 tccagaacac gcaaaagatc actaaagcga tggagatggt cgccgcttcc aaaatgcgta
     4681 aatcgcagga tcgcatggcg gccagccgtc cttatgcaga aaccatgcgc aaagtgattg
     4741 gtcaccttgc acacggtaat ctggaatata agcaccctta cctggaagac cgcgacgtta
     4801 aacgcgtggg ctacctggtg gtgtcgaccg accgtggttt gtgcggtggt ttgaacatta
     4861 acctgttcaa aaaactgctg gcggaaatga agacctggac cgacaaaggc gttcaatgcg
     4921 acctcgcaat gatcggctcg aaaggcgtgt cgttcttcaa ctccgtgggc ggcaatgttg
     4981 ttgcccaggt caccggcatg ggggataacc cttccctgtc cgaactgatc ggtccggtaa
     5041 aagtgatgtt gcaggcctac gacgaaggcc gtctggacaa gctttacatt gtcagcaaca
     5101 aatttattaa caccatgtct caggttccga ccatcagcca gctgctgccg ttaccggcat
     5161 cagatgatga tgatctgaaa cataaatcct gggattacct gtacgaaccc gatccgaagg
     5221 cgttgctgga taccctgctg cgtcgttatg tcgaatctca ggtttatcag ggcgtggttg
     5281 aaaacctggc cagcgagcag gccgcccgta tggtggcgat gaaagccgcg accgacaatg
     5341 gcggcagcct gattaaagag ctgcagttgg tatacaacaa agctcgtcag gccagcatta
     5401 ctcaggaact caccgagatc gtctcggggg ccgccgcggt ttaaacaggt tatttcgtag
     5461 aggatttaag atggctactg gaaagattgt ccaggtaatc ggcgccgtag ttgacgtcga
     5521 attccctcag gatgccgtac cgcgcgtgta cgatgctctt gaggtgcaaa atggtaatga
     5581 gcgtctggtg ctggaagttc agcagcagct cggcggcggt atcgtacgta ccatcgcaat
     5641 gggttcctcc gacggtctgc gtcgcggtct ggatgtaaaa gacctcgaac acccgatcga
     5701 agtcccggta ggtaaagcga ctctgggccg tatcatgaac gtactgggtg aaccggtcga
     5761 catgaaaggc gagatcggtg aagaagagcg ttgggcgatt caccgcgcag caccttccta
     5821 cgaagagctg tcaaactctc aggaactgct ggaaaccggt atcaaagtta tcgacctgat
     5881 gtgtccgttc gctaagggcg gtaaagttgg tctgttcggt ggtgcgggtg taggtaaaac
     5941 cgtaaacatg atggagctta ttcgtaacat cgcgatcgag cactccggtt actctgtgtt
     6001 tgcgggcgta ggtgaacgta ctcgtgaggg taacgacttc taccacgaaa tgaccgactc
     6061 caacgttatc gacaaagtat ccctggtgta tggccagatg aacgagccgc cgggaaaccg
     6121 tctgcgcgtt gctctgaccg gtctgaccat ggctgagaaa ttccgtgacg aaggtcgtga
     6181 cgttctgctg ttcgttgaca acatctatcg ttacaccctg gccggtacgg aagtatccgc
     6241 actgctgggc cgtatgcctt cagcggtagg ttatcagccg accctggcgg aagagatggg
     6301 cgttctgcag gaacgtatca cctccaccaa aaccggttct atcacctccg tacaggcggt
     6361 atacgtacct gcggatgact tgactgaccc gtctccggca accacctttg cgcaccttga
     6421 cgcaaccgtg gtactgagcc gtcagatcgc gtctctgggt atctacccgg ccgttgaccc
     6481 gctggactcc accagccgtc agctggaccc gctggtggtt ggtcaggaac actacgacac
     6541 cgcgcgtggc gttcagtcca tcctgcaacg ttatcaggaa ctgaaagaca ttatcgccat
     6601 cctgggtatg gatgaactgt ctgaagaaga caaactggtg gtagcgcgtg ctcgtaagat
     6661 ccagcgcttc ctgtcccagc cgttcttcgt ggcagaagta ttcaccggtt ctccgggtaa
     6721 atacgtctcc ctgaaagaca ccatccgtgg ctttaaaggc atcatggaag gcgaatacga
     6781 tcacctgccg gagcaggcgt tctacatggt cggttccatc gaagaagctg tggaaaaagc
     6841 caaaaaactt taacgcctta atcggagggt gatatggcaa tgacttacca cctggacgtc
     6901 gtcagtgcag agcaacaaat gttctctggt ctggtcgaga aaatccaggt aacgggtagc
     6961 gaaggtgaac tggggatcta tcctggccac gcaccgctgc tcaccgccat taagcctggt
     7021 atgattcgca tcgtgaaaca gcacggtcac gaagagttta tctatctgtc tggcggcatt
     7081 cttgaagtgc agcctggcaa cgtgaccgtt ctggccgaca ccgcaattcg cggccaggat
     7141 ctcgacgaag cgcgagccat ggaagcgaaa cgtaaggctg aagagcacat tagcagctct
     7201 cacggcgacg tagattacgc tcaggcgtct gcggaactgg ccaaagcgat cgcgcagctg
     7261 cgcgttatcg agttgaccaa aaaagcgatg taacaccggc ttgaaaagca caaaagccag
     7321 tctggaaaca ggctggcttt ttttacgcgt gacccgtcct gaagaacgtt cacatagatc
     7381 ctgctgatat aaaaccccct gttttcccgt ttattcattg atcgaaataa gagcaaaaac
     7441 atccacctga cgcttaaatg aaggtactgc cttaattttc tgcagacaaa aggtgtgacg
     7501 atggtcgaaa atggcgcttt cgtcagcggg gataatccgt tattgaacaa tttatcctct
     7561 gtccatttca cgatgaaaaa aatgtagttt tttcaaggtg aagcggttta aattcgttct
     7621 caaattacag tcaggacgcg tatgttgaat aatgctatga gcgtagtgat ccttgccgca
     7681 ggcaaaggca cgcgcatgta ttccgatctt ccgaaagtgc tgcataccct tgccgggaaa
     7741 gcgatggttc agcatgtcat tgatgctgcg aatgaattag gcgcagcgca cgttcacctg
     7801 gtttacggtc acggcggcga tctgcttaaa caggcgctga aagatgacaa cctgaactgg
     7861 gtgcttcagg cagaacagct gggtacaggt catgcgatgc agcaggccgc acctttcttt
     7921 gccgatgatg aagacatttt aatgctctac ggcgacgtgc cgctgatctc tgtcgaaaca
     7981 ctccagcgtc tgcgtgatgc taaaccgcag ggtggcattg gtctgctgac ggtaaaactg
     8041 gatgatccga ccggttatgg acgtatcacc cgtgaaaacg gcaaagttac cggcattgtt
     8101 gagcacaaag acgccaccga cgagcagcgt cagattcagg agatcaacac cggcattctg
     8161 attgccaacg gcgcagatat gaaacgctgg ctggcgaagc tgaccaacaa taacgctcag
     8221 ggtgaatact acatcaccga cattattgcg ctggcgtatc aggaagggcg tgaaatcgtc
     8281 gccgttcatc cgcaacgttt aagcgaagta gaaggcgtga ataaccgcct gcaactctcc
     8341 cgactggagc gcgtttacca gtctgaacag gctgaaaaac tgctgttagc aggcgttatg
     8401 ctgcgcgatc cggcgcgttt tgatctgcgc ggtacgctta ctcacgggcg cgatgttgaa
     8461 attgatacta acgttatcat cgagggcaac gtgactctcg gtcatcgcgt gaaaatcggc
     8521 accggttgcg tgattaaaaa cagcgtgatt ggcgatgatt gcgaaatcag tccgtatacc
     8581 gttgtggaag atgcgaatct ggcagcggcc tgtaccattg gcccgtttgc ccgtctgcgt
     8641 cctggtgctg agttgctgga aggtgcacac gtcggtaact tcgttgagat gaaaaaagcg
     8701 cgtctgggta aaggctcgaa agccgggcat ctgacttatc tgggcgatgc ggaaattggc
     8761 gataacgtta acatcggcgc gggaaccatt acctgcaact acgacggtgc gaataaattt
     8821 aagaccatta tcggcgacga tgtgtttgtt ggttccgata ctcagctggt ggccccggta
     8881 acggtaggca aaggcgcaac cattgctgcg ggtacaactg tgacgcgtaa tgtcggcgaa
     8941 aatgctctgg caatcagccg tgtgccgcag tcgcaaaaag aaggctggcg tcgtccggta
     9001 aagaaaaagt aattctggct gggaaatccg gtcatatggg atgaggagat aacataatct
     9061 cccgcccaca agcagtaact ataaaaataa ccccactctc tacaaggctc ggggcgcccg
     9121 aaaaaacggg catacaggtt gaccgacaac gatataaatc ggaatcaaaa actatgtgtg
     9181 gaattgttgg cgcgatcgcg caacgtgatg tagcagaaat ccttcttgaa ggtttacgtc
     9241 gtctggaata ccgcggatat gactctgccg gtctggccgt tgttgatgca gaaggtcata
     9301 tgacccgcct gcgtcgcctc ggtaaagtac agatgctggc tcaggcagcg gaagaacatc
     9361 ctctgcatgg cggcaccggt attgctcata ctcgctgggc gacccacggt gaaccttcag
     9421 aagctaacgc gcatccgcat gtttctgaac acattgtggt ggtgcataac ggcatcatcg
     9481 aaaaccatga accgctgcgt gaagagttaa aagcgcgtgg ctataccttc gtttctgaaa
     9541 ccgacaccga agtgattgca catttggtga actgggagct gaaacaaggc ggtactctgc
     9601 gtgaggcagt tctgcgtgct attccgcaac tgcgtggtgc gtacggtaca gtgatcatgg
     9661 actcccgtca cccggatacc ctgctggcgg cacgttctgg tagcccgctg gtgattggtc
     9721 tgggcatggg cgaaaacttt atcgcttctg accagctggc gctgttgcca gtgactcgtc
     9781 gctttatctt ccttgaagag ggcgatattg cggaaatcac tcgccgttcg gtaaacattt
     9841 tcgataaaac tggcgcggaa gtaaaacgtc aggatattga atccaatctg caatatgacg
     9901 cgggcgataa aggtatttac cgtcactaca tgcagaaaga gatctacgaa caaccgaacg
     9961 cgatcaaaaa cacccttacc gggcgcatca gccacggtca ggttgattta agcgagctgg
    10021 gaccgaacgc cgacgaactg ctgtcgaagg ttgagcatat tcagatcctc gcctgtggta
    10081 cttcttataa ctccggtatg gtttcccgct actggtttga atcgctggca ggtattccgt
    10141 gcgacgtcga aatcgcctct gaattccgct atcgcaaatc tgccgtgcgt cgtaacagcc
    10201 tgatgatcac cttgtcacag tctggcgaaa ccgcggatac cctggcgggc ctgcgtctgt
    10261 cgaaagagct gggttacctt ggttcgctgg caatctgtaa cgttccgggt tcttctctgg
    10321 tgcgcgaatc cgatctggcg ctgatgacca acgccggtac ggaaatcggc gtggcatcca
    10381 ctaaagcatt caccactcag ttaactgtgc tgttgatgct ggtggcgaag ctgtctcgcc
    10441 tgaaaggtct ggatgcctcc attgaacatg acattgtgca tggtctgcag gcgctgccga
    10501 gccgtattga gcagatgctg tctcaggaca aacgcattga agcgctggca gaagatttct
    10561 ctgacaaaca tcacgcgctg ttcctgggcc gtggcgatca gtacccaatc gcgctggaag
    10621 gtgcattgaa gctgaaagag atctcttaca ttcacgctga agcctacgct gcgggcgaac
    10681 tgaaacacgg cccgctggcg ctgattgacg ccgatatgcc ggttatcgtc gttgcgccga
    10741 acaacgagtt gttggaaaaa ctgaaatcta acattgaaga agttcgcgcg cgtggcggtc
    10801 agttgtatgt cttcgccgat caggatgcgg gttttgtgag cagtgacaac atgcacatca
    10861 ttgagatgcc gcatgtggaa gaggtgattg caccaatctt ctacaccgtt ccgctgcagc
    10921 tactggctta ccacgttgcg ctgatcaaag gcaccgacgt tgaccagccg cgtaacctgg
    10981 cgaaatcggt tacggttgag tgatatctct cgttgataga aaagggctgc cgaccggcgg
    11041 ccctttttta taagtgatat caataattcc attattc
//
LOCUS       DAZKGF010000162        11075 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZKGF010000162 DAZKGF010000000
VERSION     DAZKGF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.162"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            214..837
                     /gene="nfeR"
                     /locus_tag="ACHOQV_004046"
                     /gene_synonym="yqjI"
     CDS             214..837
                     /gene="nfeR"
                     /locus_tag="ACHOQV_004046"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFP9632332.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(941..2461)
                     /gene="aer"
                     /locus_tag="ACHOQV_004047"
     CDS             complement(941..2461)
                     /gene="aer"
                     /locus_tag="ACHOQV_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFP9632333.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLTAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            2879..4258
                     /gene="ygjG"
                     /locus_tag="ACHOQV_004048"
     CDS             2879..4258
                     /gene="ygjG"
                     /locus_tag="ACHOQV_004048"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFP9632334.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCEQVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4300..4632)
                     /locus_tag="ACHOQV_004049"
     CDS             complement(4300..4632)
                     /locus_tag="ACHOQV_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFP9632335.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            4851..5834
                     /gene="ebgR"
                     /locus_tag="ACHOQV_004050"
     CDS             4851..5834
                     /gene="ebgR"
                     /locus_tag="ACHOQV_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFP9632336.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            6018..9110
                     /gene="ebgA"
                     /locus_tag="ACHOQV_004051"
     CDS             6018..9110
                     /gene="ebgA"
                     /locus_tag="ACHOQV_004051"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFP9632337.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGEHVVHSSTIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENNLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            9107..9556
                     /locus_tag="ACHOQV_004052"
     CDS             9107..9556
                     /locus_tag="ACHOQV_004052"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFP9632338.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVIYDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            9619..11052
                     /locus_tag="ACHOQV_004053"
     CDS             9619..11052
                     /locus_tag="ACHOQV_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9632339.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
BASE COUNT         2649 a         2952 c         2795 g         2679 t
ORIGIN      
        1 aaatgcaagc gatttgtaga actgatatgt ctatagtctg ataagacgaa ccgcctcttc
       61 tcaggcatca ttactcaacg ccggatgcgg cgtgaacgcc ttatccggcc tacgtgtgag
      121 atgagtccac gtttaaaaat cattattgta cttgcagtaa tgctcatatc agatataaat
      181 tagatatatc taattaaaca aaaggaggct gatatgagcc atcatcacga agggtgctgt
      241 aaacatgaag gccagccacg ccatgagggc tgctgcaaag gtgagaagtc agaacacgag
      301 cactgcggac acggtcacca gcatgaacac ggtcaatgct gcggtggtcg ccacggtcgc
      361 ggcggcggtc gtcgacaacg tttctttggt cacggtgaat tgcgtctggt gattctggat
      421 attctctcgc gcgatgacag ccacggttac gagttgatta aagcgattga aaatctgacc
      481 caggggaatt acaccccaag cccgggcgtc atctacccaa cgctggattt tctgcaggag
      541 cagtcgctga ttaccattcg cgaagaggaa ggtggtaaga agcagattgc gctgaccgaa
      601 caaggcgcgc agtggctgga agaaaaccgc gaacaggtag agatgattga agaacgcatc
      661 aaagcgcgtt gcgttggcgc tgcgctgcgc cagaacccgc aaatgaagcg ggcgctggat
      721 aattttaaag cggtactgga tttacgcgtc aaccagagcg atatcactga tgcacaaata
      781 aaaaagatca ttgcggtgat cgaccgcgcc gcttttgata ttacgcaact ggattaatca
      841 ccgattgccg gatgtggcgt gccctacccc cgtaggcctg ataagatgcg ccagcatcgc
      901 atcaggcatt gtgcgccaac cgccggatgc ggcatatcga ttaatgcagc accgtcaccg
      961 cgtcttccag tcggctggcg cggtgtttca ccatcgccga cacctgcgca ctctcttcca
     1021 ctaactcggc gtttttctgg gtgatcaggt taagctcatc cactgcacgg gtcaggctgg
     1081 aaagcccatc ggcctgttcc agcgttgaat ggctaatctg agctatcaac tgggtgacgt
     1141 tttttacctg tgccacaata tcttccatcg tccgtccggc ggcgtgtacc tgctgcgaac
     1201 cggattgcac cttatcagca ctggcatcaa tcagcttgcg aatatcgttg gcagcattgg
     1261 cgctgcggct agctaaatga cgcacttccc ccgccaccac tgcaaaacct ttgccctgtt
     1321 cgccggcacg cgccgcttcc accgccgcat tcagggccag aatattggtc tgaaacgcaa
     1381 tatcgttaat cagcgaagta atggtgccaa tgcgctgggt actgtcggca atatcgtcca
     1441 ttgtcttgat caccgtagtc atcgcctccc caccctgcac tgccgcatta ctggcagtga
     1501 tagaaagttt atcggcagcc gacgccgtgg cagagttctg tttcaccgac gccgccattt
     1561 ggttcatggt cgccaccgtt tgctgaacgt tatcaactgt ctgctgggta tgttcgttca
     1621 gttcatcggt gcctttcgcc agcgtctcac tgccgtttct gacactggac acctggcttg
     1681 agacatcgtt aattagccaa cggcacatca ggccaagctg ccctaccgcg cgtaatgtca
     1741 gccccagctc atcgctgcga ttcagatgct caacactatt acgctctccg gtcgccacct
     1801 tcagtgcctg acgggcgaca ttttctatcg ggcggacaat ctgccattca aaacaagcgc
     1861 ttgccaataa taccactaac gcacagagga tatacgtcac caccggggca gcaacaaacc
     1921 atagcatggc cgtcagcaag ataaacatca gggtcatcac gccacgcgcc cgccagcgaa
     1981 gcggtaatga aggcagttta cccagccatc ctttacgcac caccaggcct ttatgaatac
     2041 gcttactggt acgtccggcg ttcaacgctt tgtacagcgg ctccaccgcc gcgatctctt
     2101 catccgtcgc ccgggtacga atcgacatat agccactgat ttttccctcg cgcaccatcg
     2161 gtaccgcatt ggcccgcacc caataatggt cgccattttt gcggcgattt ttcacgatgc
     2221 cgctccaggg ctcccctttt ttcagggtga accacatatc cgcaaacgcc gcttttggca
     2281 tatccgggtg acgcaccatg ttgtgcggtt gaccttgtaa ctcttgcaag gtatagccgc
     2341 tcacctgcac aaaagtgtca ttagcatgag tgatatagct ttgcagatcg gtagtggaca
     2401 tcagagtggt atcgtccgcc agcggggtat tttgctgggt gacatacgga tgagaagaca
     2461 tgttcgcgtc ctgtgcaggt tatatggttg ttaacttctt gtcagagttt atgtcggccc
     2521 cgctgcggtt atctttaact gattaatttg atttagatcg caatttgcga ttaaagcata
     2581 aatctaatcc cttgatttaa aatactttca ctctgttact atacgaaaac gttaattatc
     2641 ttgcccaaaa atcaggcagt tgttgccctg aaagtgtgca tttgcgcagc aatcgccaaa
     2701 tccatacccg acaaaaaccg tgcaaaataa caacagatgt taacaaatag cattaaatat
     2761 tgcgcaaatg ataaccacat ttgtgtttat cccgattttc gcgatcgcag ccggagtggc
     2821 gcaatccctg caatacttaa atcggtatca tgtgatacgc gagcctccgg agcatatttt
     2881 gaacaggtta ccttcgagcg catcggcttt agcgtgcagc gcccacgccc tgaatctcat
     2941 tgagaagcga acgctggatc atgaggagat gaaagcactt aaccgggagg tgattgaata
     3001 cttcaaagag catgtcaatc cggggttttt agagtatcgc aaatctgtta ccgctggcgg
     3061 ggattacgga gccgtagagt ggcaagcggg gggtttaaat acgcttgtcg acacccaggg
     3121 acaggagttt atcgactgcc tgggaggttt tggaattttc aacgtggggc accgtaatcc
     3181 agttgtggtt tccgccgtac agaatcaact tgctaaacaa ccgcttcaca gccaggagct
     3241 gctcgatccg ttacgggcga tgctggcgaa aacccttgcg gcgctaacgc ccggtaaact
     3301 gaaatacagc ttcttctgta atagcggcac cgaatccgtt gaagcggcgc tgaagctggc
     3361 gaaggcttat cagtcaccgc gcggcaagtt tacttttatt gccaccagcg gcgcgttcca
     3421 cggtaaatca cttggcgcgc tgtcggccac ggcgaaatcg accttccgca aaccttttat
     3481 gccactgctg ccagggtttc gtcatgtacc gtttggcaat atcgaagcca tgcgcacggc
     3541 tcttaacgag tgcaaaaaaa ccggtgatga tgttgctgcg gtgatcctcg aaccgattca
     3601 gggcgaaggt ggcgtcattc tgccgccgcc gggatatctc accgccgtgc gtaagctgtg
     3661 cgatgagttc ggcgcactga tgatcctcga tgaagtgcaa accggtatgg ggcgcacggg
     3721 caagatgttc gcctgcgagc atgagaacgt acagccggac atcctgtgtc tggcaaaagc
     3781 cctcggcggc ggcgtgatgc cgattggcgc aaccatcgcc actgaagagg tgttttccgt
     3841 tctgttcgac aacccgttcc tgcataccac cacctttggc ggcaacccgc tggcctgtgc
     3901 ggcggcgctg gcgaccatca atgtgttgct ggagcagaac ttaccggctc aggctgaaca
     3961 aaaaggcgat atgttgctgg acggttttcg tcaactggcg cgggaatatc ccgatctggt
     4021 acaggaagcg cgtggtaaag ggatgttgat ggcgattgag tttgttgata acgaaatcgg
     4081 ctataacttt gccagcgaga tgttccgcca gcgcgtcctg gtggccggaa cactcaacaa
     4141 cgctaaaacg atccgcattg aaccgccact gacactgacc attgaacagt gtgaacaggt
     4201 gatcaaagcg gcgcgtaagg cgctggcggc catgcgagtc agtgtcgaag aagcgtagta
     4261 tcaaatcgga tggcgatacg acgtcgccat ccgatctttt tatacgatac gtacgcccgc
     4321 aggcatcatc cgctccggcg ttaacagcac gctttcactg ccgtcatcgg tttccgcaca
     4381 caacagcatg cattccgacg tttcaccgcg cattttcgct ttttgcaaat tgcataagac
     4441 caccactgtt tttcccatca gctcttcttc gctgtagtac ggcaccaggc tggtcacggt
     4501 ttgcagcgtt ttttccccca catcaacctg tacgatgtac agcttgtcgg cgttttcatg
     4561 gcgtttcact tccacaatct ttccgacgcg catttccaga cgtgcaaaat cagcgtaagc
     4621 cacggtttcc attgtttcct cccttcgagt aaaattttac taaactatag caaaagtttt
     4681 tctcaatcct gtaggctaaa aatggagaat gcaggcgtga tcacattcgc aagccgctgt
     4741 gttaccgtta cagcgtcaaa gaaacgcgct ttatttactg aaaacaggtg acccgataag
     4801 cacttcctct acaatggggg cgcacatcag ggaaagtaaa aaaggtaaac atggcaacac
     4861 taaaagacat cgcaatcgaa gctggcgtat ccctggcgac agtatccagg gtcttgaatg
     4921 acgatccgac attgaatgtg aaagaagaga cgaaacatcg cattctcgag atcgccgaaa
     4981 agctggagta caagaccagt agcgcccgta aactccagac aggcgcagtc aaccaacacc
     5041 atattctggc tatctacagc taccaacagg agctggagat caacgatcct tactatctgg
     5101 cgatccgcca cggcattgaa actcagtgcg aaaagctggc gatcgaactg accaactgtt
     5161 atgaacacaa cggcttacca gacattaaaa acgtcaccgg tattttaatt gtcggcaaac
     5221 ccacgtccgc cctgcgcgcc gccgcctgtg cgttgaccga caatatctgt tttatcgact
     5281 ttcacgaacc cggcagcggt tacgatgcgg tggatatcga tctggcacgt atcagtaaag
     5341 aaatcatcga tttctatatc aatcagggcg ttaatcgtat tggttttatt ggcggtgaag
     5401 acttgcctgg caaggcggat attcgtgagg tcgcctttgc ggaatatggt cgtctgaaac
     5461 acgtggtacg cgaagaggat atctggcgcg gcggtttttc cagttcgtcg ggctatgaac
     5521 tggcaaaaca gatgctggcg cgggaagact atccgaaggc actgtttgtt gcttccgatt
     5581 ccattgctat cggcgtactg cgggcaattc atgaacgtgg cctgaacatc ccacaggata
     5641 tttcgcttat cagcgttaac gatatcccca ccgcgcgatt tacctttccg ccgctctcca
     5701 ccgtgcgcat ccattccgag atgatgggaa gtcagggcgt taacctggtg tatgaaaaag
     5761 cccgcgatgg tcgcgcgctg ccgctgttgg tcttcgttcc cagcaaatta aaactgcgcg
     5821 gcacgacccg ttaaatcccc ttgcacgctg tccggcaatc gtttttgccg gacagtgctg
     5881 ccgtttattt tcgtgatcca gttaaagtaa atgcatttat ccgctacttt ttagtaaaat
     5941 ttttactaaa ctccccagca attacacaaa ctaccatcac cataaatggt tccgatttct
     6001 ctctaccggg aggccctatg aatcgctggg aaaacattca gctcacccac gaaaaccgac
     6061 ttgcgccgcg tgcgtacttt ttttcatatg attctgttgc gcaggctcgt acttttgccc
     6121 gtgaaaccag cagcctgttt ctgcccttaa gcggtcagtg gaatttccac ttttttgacc
     6181 atccgctgca agtgccagaa gccttcacct ctgagttaat ggctgactgg gggcatatta
     6241 ccgtccccgc catgtggcaa atggaaggtc acggcaaact gcaatatacc gacgaaggtt
     6301 ttccgttccc catcgatgtg ccgtttgtcc ccagcgataa cccaaccggt gcctatcaac
     6361 gcattttcac cctcagcgac ggctggcagg gtaaacagac gctgatcaaa tttgacggtg
     6421 tcgaaaccta ttttgaagtc tacgttaacg gtcagtatgt ggggttcagc aaaggcagtc
     6481 gcctgaccgc agagtttgac atcagcgcga tggttaaaac cggcgacaac ctgttgtgtg
     6541 tgcgcgtgat gcagtgggcg gactctacct acgtggaaga ccaggatatg tggtggtcgg
     6601 cggggatttt ccgcgatgtt tatctgatcg gaaaacaact cacgcatatt aacgatttca
     6661 ccgttcgtac cgactttgac gaagcctatt gcgatgccac actttcctgc gaagtggtgc
     6721 tggaaaatct cgccgcctcc cctgtcgtca cgacgctgga atataccctg tttgatggcg
     6781 aacacgtggt gcacagcagc accattgatc atttggcaat tgaaaaactg accagcgcca
     6841 gttttgcttt tactgtcgaa cagccgcagc aatggtcagc agaatcccct tatctttacc
     6901 atctggtcat gacgctgaaa gacgccgacg gcaacgttct ggaagtggtg ccacaacgag
     6961 ttggcttccg tgatatcaaa gtgcgcgacg gtctgttctg gatcaataac cgttatgtga
     7021 tgctgcacgg cgtcaaccgc cacgacaacg atcatcgcaa aggccgcgcc gttgggatgg
     7081 atcgcgtcga gaaagatctc cagttgatga agcagcacaa catcaactcc gtgcgtaccg
     7141 ctcactaccc gaacgatcca cgtttttacg aactgtgtga tatctacggc ctgtttgtga
     7201 tggcggaaac cgacgtcgaa tcgcacggct ttgctaatgt cggcgatatc agccgtatta
     7261 ccgacgatcc gcagtgggaa aaagtttacg tcgagcgcat tgttcgccat attcacgcgc
     7321 agaaaaacca tccgtcgatc atcatctggt cgctgggcaa tgaatccggc tatggctgta
     7381 acatccgcgc gatgtaccat gcggcgaagg cgctggatga cacgcgactg gtgcattacg
     7441 aagaagatcg cgatgctgaa gtggtcgata ttatttccac catgtacacc cgcgtgccgc
     7501 tgatgaatga gtttggtgaa tacccgcatc cgaagccgcg catcatctgt gaatatgctc
     7561 atgcgatggg gaacggaccg ggcgggctga cggagtacca gaacgtcttc tataagcatg
     7621 attgcattca gggacattat gtctgggaat ggtgcgacca cgggatccag gcgcaggatg
     7681 acaacggcaa tgtctggtat aaattcggcg gcgactacgg cgactatccc aacaactata
     7741 acttctgtct tgatggtttg atctattccg atcagacgcc gggaccgggc ctgaaagagt
     7801 acaaacaggt tatcgcgcca gtaaaaatcc acgcgctgga tctgactcgc ggcgagttga
     7861 aagtcgaaaa taatctgtgg tttaccacgc ttgatgacta caccctgcac gcagaggtgc
     7921 gcgccgaagg tgaaacgctc gcgacgcaac agattaaact gcgcgacgtt gcgccgaaca
     7981 gcgaagcccc cttacagatc acgctgccac agctggacgc ccgcgaagcg tttctcaaca
     8041 ttacggtgac caaagattcc cgcacccgct acagcgaagc cgggcattct atcgccactt
     8101 atcagttccc gctgaaggaa aacaccgcgc agcctgtacc tttcgcaccg aataacgccc
     8161 gtccgctgac gctggaagac gatcgtttga gctgtactgt tcgcggctac aactttgcga
     8221 tcaccttctc aaaaacgagt ggtaaaccga catcctggca ggtgaatggc gagtcgctgc
     8281 taacccgcga gccaaaaatc aacttcttca agccaatgat cgacaaccac aagcaggagt
     8341 acgaagggct gtggcagccg aatcatttgc agatcatgca ggaacatctg cgcgactttg
     8401 ccgtagaaca gagcgatggt gaagtgttga tcatcagccg cacggttatt gccccaccgg
     8461 tgtttgactt cgggatgcgc tgcacctaca tctggcgcat cgctgccgat ggccaggtta
     8521 acgtggcgct ttccggcgag cgttacggcg actatccgca catcattccg tgcatcggtt
     8581 tcaccatggg gattaacggc gaatacgacc aggtggcgta ttacggtcgt ggaccgggcg
     8641 aaaactacgc cgacagccag caggctaaca tcatcgatat ctggcgcagc accgtcgatg
     8701 ccatgttcga gaactatccc ttcccacaga acaacggcaa ccgtcagcat gtccgctgga
     8761 cggcactgac taaccgccac ggcaatggtc tgctggttgt tccgcagcgc cccattaact
     8821 tcagcgcctg gcgctatacc caggaaaaca tccacgctgc ccagcactgt aacgagttgc
     8881 agcgcagtga tgacatcacc ctgaatctcg accaccagct gcttggcctc ggctccaact
     8941 cctggggcag cgaggtgctg gactcctggc gcgtctggtt ccgtgacttc agctacggct
     9001 ttacgttgct gccggtttct ggcggagaag ctaccgcgca aagcctggcg tcgtatgagt
     9061 tcggcgcagg gttcttttcc acgaatttgc acagcgagaa taagcaatga ggatcatcga
     9121 taacttagaa cagttccgcc agatttacgc ctctggcaag aagtggcaac gctgcgttga
     9181 agcgattgaa aatatcgaca acattcagcc tggcgtcgcc cactccatcg gtgactcatt
     9241 gacttaccgc gtggagacgg actccgcgac cgatgcgcta tttaccgggc atcgacgcta
     9301 ttttgaagtg cattactacc tgcaagggca gcaaaaaatt gaatatgcgc cgaaagagac
     9361 attacaggtc gtggaatatt atcgtgatga aactgaccgt gaatatttaa aaggctgcgg
     9421 agaaaccgta gaggtccacg aagggcaaat cgttatttac gatatccatg aagcatatcg
     9481 gtttatctgc aataacgcgg tcaaaaaagt ggttctcaaa gtcaccatcg aagatggtta
     9541 tttccataac aaataacaac tacggcggca aaaggagttt gccgccaccg ctaccctact
     9601 cattttcgga gatgtgttat gtctgatacc aaacgtaata caatcggcaa attcggcttg
     9661 ctctcgctga cttttgccgc cgtttacagc tttaacaacg ttatcaataa taatattgag
     9721 cttggactgg cctcggcacc gatgtttttc ctcgcgacga ttttttattt tattcccttc
     9781 tgtctgatca tcgcagaatt tgtttcgtta aataaaaact cagaagccgg tgtctacgcg
     9841 tgggtaaaaa gttcgctggg cgggcgttgg gcatttatta ctgcctatac ctactggttc
     9901 gtaaacctgt tctttttcac ctcactgttg ccgcgcgtta ttgcttatgc ttcgtatgcc
     9961 ttcctcggct acgaatatat tatgacgccg gttgccacca ccattatcag tatggtgctg
    10021 ttcgccttct ccacctgggt ttccaccaac ggggcgaaaa tgctggggcc aattacctcc
    10081 gtcacttcaa cgctgatgct gctgttaacg ctctcctaca ttttactggc aggtacggca
    10141 ctggttggcg gcgtacagcc tgccgatccg atcaccgttg acgcgatgat cccgaacttc
    10201 aactgggcgt tcctcggcgt taccacctgg atctttatgg ccgcaggtgg cgcggagtcc
    10261 gtcgcggtgt acgttaacga cgtcaaaggc ggttcgaaat cgttcgttaa agtgatcatc
    10321 ctcgccggga tttttatcgg cgtactgtat tccgtttcct cggtgctgat taacgtcttc
    10381 gtcagcagca aagagttgaa atttactggc ggatcggtgc aggtattcca cggcatggcg
    10441 gcgtattttg gtctgccgga agcgttgatg aatcgctttg tcggtctggt gtcctttacc
    10501 gcgatgttcg gttccctgct gatgtggacc gcaacgccgg tgaaaatttt cttctccgaa
    10561 atcccggaag gcatctttgg taagaaaacc gtcgaactga acgaaaacgg cgttccggcg
    10621 cgcgcagcgt ggatccagtt cctgatcgtc atcccgctga tgattatccc gatgctcggt
    10681 tccaataccg tgcaagatct gatgaatacc attattaata tgaccgccgc agcgtccatg
    10741 cttccgccgt tattcatcat gctggcttac ctgaatttac gcgccaaatt agatcacctg
    10801 ccacgcgatt tccgtatggg ctcccgccgc accggtatta tcgttgtttc aatgctgatt
    10861 gcgatatttg ccgtagggtt tgtcgcttcg acattcccga ctggcgcgaa tattctgacc
    10921 atcatttttt ataacgtcgg cggtattgtt atcttcctcg gctttgcgtg gtggaaatac
    10981 agtaaatata taaagggatt aacggctgaa gagcgccata ttgaagcgac gccagccagc
    11041 aatgttgatt aagcagaata ataaaaaatg agcag
//
LOCUS       DAZKGF010000163        11040 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZKGF010000163 DAZKGF010000000
VERSION     DAZKGF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.163"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            179..1393
                     /gene="manD"
                     /locus_tag="ACHOQV_004054"
     CDS             179..1393
                     /gene="manD"
                     /locus_tag="ACHOQV_004054"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFP9632340.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTASGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1460..2743
                     /locus_tag="ACHOQV_004055"
     CDS             1460..2743
                     /locus_tag="ACHOQV_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9632341.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            2995..3912
                     /gene="oxyR"
                     /locus_tag="ACHOQV_004056"
     CDS             2995..3912
                     /gene="oxyR"
                     /locus_tag="ACHOQV_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFP9632342.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3895..5295)
                     /gene="sthA"
                     /locus_tag="ACHOQV_004057"
     CDS             complement(3895..5295)
                     /gene="sthA"
                     /locus_tag="ACHOQV_004057"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFP9632343.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5627..6277
                     /gene="fabR"
                     /locus_tag="ACHOQV_004058"
     CDS             5627..6277
                     /gene="fabR"
                     /locus_tag="ACHOQV_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFP9632344.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6277..6636
                     /gene="yijD"
                     /locus_tag="ACHOQV_004059"
     CDS             6277..6636
                     /gene="yijD"
                     /locus_tag="ACHOQV_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFP9632345.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLL
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6676..7776)
                     /gene="trmA"
                     /locus_tag="ACHOQV_004060"
     CDS             complement(6676..7776)
                     /gene="trmA"
                     /locus_tag="ACHOQV_004060"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFP9632346.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7890..8080
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8145..10028
                     /gene="btuB"
                     /locus_tag="ACHOQV_004061"
     CDS             8145..10028
                     /gene="btuB"
                     /locus_tag="ACHOQV_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFP9632347.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQI
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKSINNVNQYINVKSATIKGLEWTGNITTGPVEH
                     RLTLQYVDPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     gene            9973..10830
                     /gene="murI"
                     /locus_tag="ACHOQV_004062"
     CDS             9973..10830
                     /gene="murI"
                     /locus_tag="ACHOQV_004062"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP9632348.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2587 a         2577 c         2925 g         2951 t
ORIGIN      
        1 tgttgtttta acgatgatag ctaattattc aggtggtttt tgagggtgtt ttttctcctc
       61 ggtagaaatt tttattttta atgtgatcat ggttgaataa tcgctctttc tctcttgtat
      121 ggtagtaggc gcaaaattaa gtttgaaacg tctatttaca acacaggaga ttgcaataat
      181 gaaaattatt gctgcagatg tttttgttac ttgcccaggg cgtaatttcg ttacgcttaa
      241 aataaccact gcaagcggac tttgtggatt aggagatgcc acgttaaatg gtcgcgaatt
      301 gtcggtcgct tcttatctga aagatcatct ttgtccacaa ctaattggcc gcgatgccag
      361 ccgtatcgaa gatatctggc aattctttta taaaggtgct tactggcgac gtggccccgt
      421 cacgatgtca gccatttctg ccattgatat ggcgttgtgg gatatcaaag caaaagcggc
      481 aaatatgcca ttgtatcaac tgcttggtgg tgcttctcgt gaaggcgtta tggtttattg
      541 ccacaccacc gggcgtacga ttgatgaagt ccttgaagac tatgcaaagc accagcagat
      601 gggctttaag gcgattcgtg tgcagtgtgg tgtaccgggt atgcaaacca cctatggtct
      661 ggcgaagggc aaaggactgg cgtatgaacc agcaaccaaa ggcctgtggc cagaagagca
      721 gttgtggtct tcagaaaagt atctcgattt tacgccgaag ttatttgaag cggtgcgtaa
      781 taagttcggt tttaacgaac atctgttgca tgacatgcac catcgcctga cccccatcga
      841 agcggcgcgc tttgggaaga gcattgaaga ttaccgcctg ttctggatgg aagatccgac
      901 gcctgccgag aatcaggaat gttttcgtct gattcgtcag cataccgtga cgccgattgc
      961 ggtcggtgaa gtgtttaaca gtatctggga ctgcaagcag ttaatcgaag agcagctgat
     1021 tgactatatt cgcacgacga ttacccatgc aggcggcatt accgggatgc gacggattgc
     1081 cgattttgca tcgctttatc aggtacgtac cggttcgcat ggcccttcag atttatcgcc
     1141 tgtttgccat gcggcggcgc tgcattttga cctctgggtg ccaaactttg gcgttcagga
     1201 gtacatgggc tattccgaac aaatgcttga agtcttcccg catagctggc gcttcgatga
     1261 aggttatatg cacccaggcg atgaaccggg gctgggtatt tctttcgatg agaaactggc
     1321 ggcgaaatat ccgtatgacc cggcgtatct ccccgtcgca cgactggaag atggcacatt
     1381 gtggaactgg taatagcggt tcgcttaatt attttctgaa tattcatcaa atcgattaat
     1441 tccgctcaag gagctaaaaa tggacgttga tgtgtcaacc agtgttgcgg gtaataaacc
     1501 gcaacggatt cgtcgtattc agacagtgac cctggtttta ttatttatgg cggggatcgt
     1561 taattttctc gaccgttcgt cattgagcgt ggcaggtgaa gcaattcgtg gcgagttagg
     1621 attatccgct accgagtttg gtgttttgct ttctgcattt tctctgtctt atggtttttc
     1681 acaacttcct tccggtattt tgctagaccg ctttggccca cgaatagtgt taggcgcagg
     1741 cttaatattc tggtcattaa tgcaggcatt aacaggaatg gttaattctt ttagccactt
     1801 tattttaatg cgtatcggtc tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa
     1861 gtcgatcacc gactggtatg cgcaaaaaga acgcggcacg gcgctgggga tttttaactc
     1921 atctaccgtt atcggtcagg ctatcgcgcc tcctgctctt gtattgatgc aactggcatg
     1981 gggctggcgg acgatgtttg tcatcatcgg cgtagcaggg attctggttg ggatctgttg
     2041 gtacgcgtgg tatcgcaacc gggcgcagtt tgtcctgacc gaggaagaac ggacatatct
     2101 ctccgcgccg gttaaaccgc gtccacagtt acaatttagc gagtggctgg cgctgtttaa
     2161 gcatcggaca acctggggga tgattttagg tttctctggc gtcaattata ccggttggct
     2221 ctacatcgcg tggttacccg gttatttgca ggccgaacaa ggtttcagtc tggcgaaaac
     2281 cggttgggtg gcggcaattc ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat
     2341 tgttgtcgat cggctggcga aaaaaggcta tgacctggcg aagacgcgta aaacggcgat
     2401 tgtctgcggt ttgatgatgt cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc
     2461 agcccaggcg gtagcgttta tctcaatggc gctgttctgc gtgcatttcg cgggaacatc
     2521 tgcatggggg ctggtgcagg tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat
     2581 tcaaaacttt ggcagttttg tttttgcttc ctttgctccg atcgtaaccg gttgggtcgt
     2641 ggataccaca cactcgttta atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg
     2701 ggcgctgtgt tacttcttta ttgtcaaaga tcggattgag taaccgttac tatcaggctc
     2761 tcttgctgtg ggcctgtaga ataaaaaaaa gcggatcctg gagatccgca aaagttcacg
     2821 ttggctttag ttattcgagt tgagaaactc tcgaaacggg cagtgacttc aagggttaaa
     2881 agaggtgccg ctccgtttct gtgagcaatt atcagtcaga atgcttgata gggataatcg
     2941 ttcgttgcta tgctacctat cgccgcgaac tatcgtggca atggaggatg gataatgaat
     3001 attcgtgatc ttgagtacct ggtggcattg gccgaacacc gccattttcg gcgtgcggca
     3061 gattcctgcc acgttagcca gccgacgctt agcgggcaaa ttcgtaagct ggaagatgag
     3121 ttgggtgtga tgttgctgga gcggaccagc cgtaaagtgt tattcaccca ggcgggaatg
     3181 ctgctggtgg atcaggcgcg taccgtgctg cgtgaggtga aagtccttaa agagatggca
     3241 agccagcagg gcgagacgat gtccggaccg ctgcatattg gtctgattcc cacggttgga
     3301 ccgtacctgc taccgcatat tatcccgatg ctgcaccaga cctttccaaa gctggaaatg
     3361 tatctgcatg aagcacagac ccaccagtta ctggcgcaac tggacagcgg caaactcgat
     3421 tgcgtgatcc tcgcgctggt gaaagagagc gaagcattca ttgaagtgcc gttgtttgat
     3481 gagccaatgt tgctggctat ctatgaagac catccgtggg cgaaccgcga atgcgtaccg
     3541 atggccgatc tggcagggga aaaactgctg atgctggaag atggtcactg tttgcgcgat
     3601 caggcaatgg gtttctgttt tgaagccggg gcggatgaag atacacactt ccgcgcgacc
     3661 agcctggaaa ctctgcgcaa catggtggcg gcaggtagcg ggatcacttt actgcctgcg
     3721 ctggctgtgc cgccggaacg caaacgtgat ggggttgttt atctgccatg cattaagccg
     3781 gaaccacgcc gcactattgg cctggtttat cgtcctggct caccactgcg cagccgctat
     3841 gagcagctgg cagaggccat ccgcgcaaga atggatggcc atttcgataa agtgttaaaa
     3901 caggcggttt aagccattta gcgcagctac ccgataggct tccgccatcg tcgggtagtt
     3961 aaaggtggtg ttgacgaagt actcaatagt gttgccgcca cctttctgtt ccataatcgc
     4021 ctgaccgata tgaataattt cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc
     4081 ttttgtttcc cgatggaaca aaattttcag cgtgcccacg ttcatgccga cgatttgtgc
     4141 acgtgccaga tgtttaaact gggcgcggcc cacttcatat ggcactttca tcgcggtcag
     4201 ctgctgttcg gttttgccca cagagctgat ttccgggatg gtgtaaatac cggtagggat
     4261 atcttcaatc agatgtgcgg tggcttcacc tttcaccagc gcctgcgcgg caatgcgccc
     4321 ctggtcatag gccgccgacg ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac
     4381 gtgcggctgt gcggtctgat acatgctgtt gaccttcagc tgtccgcggc tgtcagtttc
     4441 cagcccaatg ttctgtaacg ccagcgaatc ggtattaccg gtgcgaccgt tggcatagag
     4501 caggcagtca gctttcagtt ttttacccga cttcaaatgc atgatcacac cgtcgtcaca
     4561 gccttcgatc ttctcgtact cttcgttgtg acgaatcact acgccactgt tccagaagtg
     4621 ataggagaga gaatctgaca tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt
     4681 gatcagatcc acttttacat ccataccgcg gaagatcgac gcatattcac agccgatcac
     4741 tccagcacca tagataagta catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct
     4801 gtcgtaaatg cgtggatgcg tgaaatcaac atctgttgga tgatatggac gagagccgca
     4861 agcaataaca aatttttcag cggttagtgt ctcgacgctg ccgtccgggc aatccagcgc
     4921 cagcgtgtgc tcgtcaacaa agcgagcgtt cccctgcaat atttcacagt gattacgttc
     4981 gtaaaatccc tgacgcatgc gcgtttgttg attaatcacg ttatcggcat ggttaaggat
     5041 atcggcaaaa gaagagcgga gcagtcgcga atggtcgctg taaagtgggt tttgattaaa
     5101 ttctataatg cggctgacgg cgtgacggag agctttcgac gggatggtgc cccagtgggt
     5161 gcaaccgccg ccaacatttt gataacgctc gataactgcg acgcgcgcac cttgcttaac
     5221 caggcccatt gcagcgcctt cgccgccggg gccggaacct attactatgg catcgtaatc
     5281 gtaggaatgt ggcatggtag ggcttacctg ttcttataca taaaagcaac agaatggtaa
     5341 cattttatcg cgggtaagcc aattgatccc cgtcatttat ctggctatat cgtgagcggc
     5401 ctttgctttg tctgtttctt acttttgacc tgaacgtttt attggatttt tatcgacgat
     5461 actctccgtt taagcggcag gtttccgttg tacgtaaaag aaccggccaa agaattgcag
     5521 taaatatgtt ttattgcgtt accgttcatt cacaatactg gagcaatcca gtatgttcat
     5581 tctctggtat agtgccagca gtacttttgg caaggattca gacatcgtga tgggcgtaag
     5641 agcgcaacaa aaagaaaaaa cccgccgttc gctggtggaa gccgcattta gccaattaag
     5701 tgctgaacgc agcttcgcca gcctgagttt gcgtgaagtg gcgcgtgaag cgggcattgc
     5761 tcccacctct ttttatcggc atttccgcga cgtagacgaa ctgggtctga ccatggttga
     5821 tgagagcggt ttaatgctac gccaactcat gcgtcaggcg cgtcagcgta tcgccaaagg
     5881 cgggagtgtg atccgcacct cggtctccac atttatggag ttcatcggta ataatcctaa
     5941 cgccttccgg ttattattgc gggaacgctc cggcacctcc gctgcgtttc gtgccgccgt
     6001 tgcgcgtgaa attcagcact tcattgcgga acttgcggac tatctggaac tcgaaaacca
     6061 tatgccgcgt gcgtttactg aagcgcaagc cgaagcaatg gtgacgattg tcttcagtgc
     6121 gggggccgag gcgttggatg tcggcgtcga acaacgtcgg caattagaag agcgactggt
     6181 actgcaactg cgaatgattt ctaaaggggc ttattactgg tatcgccgtg aacaagagaa
     6241 aaccgcaatt attccgggaa atgtgaagga cgagtaatga aacaagcaaa tcaagataga
     6301 ggtacgctgc tgctggcgtt ggttgctggc ttatcgatta atggtacttt cgcagcgctg
     6361 tttagctcca ttgtgccatt ttctgtattc ccgattattt ccctgttgct gacggtttac
     6421 tgcctgcatc aacgttatct taatcgcacc atgccggtag gcttgccggg tctggcagct
     6481 gcctgtttta ttctcggcgt actgctgtac agcacggtag ttcgtgcgga atatccggat
     6541 atcggctcta acttcttccc ggcagtactc tccgtcatta tggtgttctg gattggcgcg
     6601 aagatgcgta accgtaagca ggaagttgct gagtaatcgg tatcatgccg gatgctggtg
     6661 catccggcat gggttttact tcgcggtcag taatacgccg cactccatat ggtgcgtgta
     6721 ggggaactga tcaaacagag ccagacgttc gaccttgtgc gtctggctta atgtttccag
     6781 attcttgcat aacgtttccg gattgcagga gatatacaga atacgtggat acgcctgcac
     6841 cattttctcg gtttcactgt ccagaccgct gcgcggtggg tcgacaaaaa tggtttcgca
     6901 ctgataactc tttaagtcga ttccttgcag gcggttaaac tcgcgtacgc cattcatcgc
     6961 ctgagtaaat tcttctgccg ccatacgaat aatttgtacg ttatcaatat ggttagcggc
     7021 aatgttgtat tgcgcggcag cgaccgacgg cttagcgatt tcggtggcta atacccgatc
     7081 aaaattgcgc gccagtgcta atgaaaagtt accgttgccg cagtacagct ccagcaaatc
     7141 gccttttgag cctttggtta cgtccagcgc ccattccagc atctgaatat tcatcgccgc
     7201 gttcggctgg gtaaagctgt tttctacctg acggtagatc atctctttcc ctgcgaccgg
     7261 cagacgttca tcgatgtaat cctggtccag ctcgattttg gttttcgttg cccgaccaat
     7321 cagatgcaca ttcagattct gcgctcgcag tgcatcgcgc agggcttccg cttcctgacg
     7381 ccactcatca tccagcttct tatggtacag cagggaaacc accgcctgat tactcagcgt
     7441 ggtgaggtaa tcaatctgga acaacttgtg gcgcagaacg ggattattac gcacacccgc
     7501 aatcatcgcc gtcatcaact ggttgataag ttcactggcg gcggggaagc tatccacgcg
     7561 gatgcggctt ttggtttgtt gatcgaaaat gatgtgatac aggtcatcgc catcgtgcca
     7621 gatgcggaac tccgcacgca tccggtaatg actgaccggc gagcgaaaca cttccggaac
     7681 caggtcagaa aacggtgcca tcatactttg caaacgtacc actttttcgg ctaactgcgc
     7741 ttcatactgt tctgttggaa ggtgttcggg ggtcatgatg tatcctgaaa aattaaagta
     7801 cgcggcgatt gtagggattg ctcatcagat gtccagatct tgatgaattc ctatttgtga
     7861 gctacgtctg gacagtaact tgttacaacc tgttgcatcc acttgccggt cctgtgagtt
     7921 aatagggaat ccagtgcgaa tctggagctg acgcgcagcg gtaaggaaag gtgcgatgat
     7981 tgcgttatgc ggacactgcc atccggtggg aagtcatcat ctcttagtat cttagatacc
     8041 cctccaagcc cgaagacctg ccggctaacg tcgcatctgg ttctcatcat cgcgtaatat
     8101 tgatgaaacc tgcggcatcc ttcttctatt gtggatgctt tacaatgatt aaaaaagctt
     8161 cgctgctgac ggcgtgttct gtcacagcct tttccgcttg ggcacaggat accagcccgg
     8221 atactctcgt tgttactgct aaccgttttc agcagccagt aaatactgtc cttgcaccta
     8281 ctgatattgt tacccgtgag gatattcaac gctggcagtc gaaagacctc aacgatgtta
     8341 tgagtcatct ccccggtgta aatatctctc aaactggtgg aatggggacg agttcttctc
     8401 tttatgttcg aggcactgaa tcgcgccatg ttttagtgtt gattgacggt gtgccgatgg
     8461 cccgtgccgg tatcgacaat gcaattgata ttagccagtt cccggtatca cttgtacagc
     8521 gtgttgaata tattcgtggg ccgcgttctg ccgtttatgg ttctggtgcc atcggaggtg
     8581 tggtgaacat tattacaatg agtaatgatg agaaagctca gatcaacgca ggaatgggta
     8641 caaatggtta tcaagcctat gatggggtaa taaataaacg ttttggcgat acgatggtaa
     8701 cagcagcagg tgcttaccag acgaccaaag gttttaacat tcagcctggc tcaccataca
     8761 gtggtgatag cgatcgcgat ggatatcgca acaaactttt ctgggggggg ctgcagcata
     8821 agtttaacga taacttctcg gggttcttcc gtggttacag ctacacagct aataatgatt
     8881 acgaccaggg ctcgatgtat ggttctgcta ctggcaacga agaagctcag aattacaacc
     8941 agtcctggga tactggccta cgctataact ccggcatcta ttcgtctcag ttaattgcta
     9001 actatcaacg actcaagaat tataactaca caaataatct tggtcgttat gcgggagatg
     9061 ccaccctgga tgacatggaa cagcgttata ttcagtgggg taatagcatt gaggtagggc
     9121 atggtgcaat tagcggcggg atcgattgga aacaggaaaa actgacgtcc agcagcatta
     9181 caaaatcaga tgattacgaa cgcgatacca ccgggttata cctgactgga cagcaacaga
     9241 tttccaatgt gactttggaa gcttctggcc gagaagatca tgatgaacag tttggctggc
     9301 atggtacctg gcagacagct gctggctggg agtttattga tggctaccag gcaacacttt
     9361 cttacggtac cagttttctt tcgccttctc ttggacagca atacggggct gctcgctttg
     9421 cttcaattta tggtcctggt attgcagcca acccaaattt gaagccagaa gaatctaaac
     9481 agtgggagat tggaattgag ggggtaactg ggactctgga ttggcggctc tcaggatatc
     9541 gttatgaaat tcagaacctg attgattata aaagcataaa taatgtcaac cagtacatta
     9601 atgtgaaatc agccacgatt aaagggctgg aatggacggg aaatattaca accggtcctg
     9661 ttgaacatcg cttgacgtta caatatgttg acccgcgcga tgatgaaaca gataaagtgc
     9721 tgtaccgtcg ggcaaaacag caggttaagt acgagttgac tggacaagct tatggtttcg
     9781 aatgggacgt atcttaccaa tatataggtc aacgatacga caacgcttat gacgagacct
     9841 ctggctcatc acatacagtt aaattaggcg gtgtgagctt gtgggatctt gcggttgcgt
     9901 atccggtcac ctctcatcta acagttcgtg gtaaaatagc caacctgttc gacaaagatt
     9961 atgagacagt ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca
    10021 ccttctgaac cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc
    10081 tatgacgaga tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc
    10141 gctttcccgt atggcgaaaa aagcgaagag tttattgttg agcgagtggt ggcaattgtc
    10201 accgcagtgc aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt
    10261 accgtttcac ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg
    10321 gcgattaaac ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc
    10381 ggaactgtta aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag
    10441 atagaaatgc tgggctcggc agagatggtc gagttggctg aagcgaagct gcatggcgaa
    10501 gatgtttctc tggatgcgct aaaacgtatc ttgcgtccgt ggttaagaat gaaagagccg
    10561 ccagataccg ttgtactggg gtgtacccat ttccctctac tacaagaaga actgttacag
    10621 gtgctgccag agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc
    10681 tggctgttag aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt
    10741 atggcaatga cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa
    10801 acgctcgaaa aactggcagt tttaggctga tttggttgaa tcttgcgcgg tcagaaaatt
    10861 attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg
    10921 gaacaacggc aaacacgccg ccgggtcagc ggggttctcc tgagaatctc aacagagaaa
    10981 agcaaaaaaa tgcttgactc tgtagcggga aagcgtatta tgcacacccc gcgccgctga
//
LOCUS       DAZKGF010000164        10956 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZKGF010000164 DAZKGF010000000
VERSION     DAZKGF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.164"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            59..1201
                     /gene="yghO"
                     /locus_tag="ACHOQV_004063"
     CDS             59..1201
                     /gene="yghO"
                     /locus_tag="ACHOQV_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFP9632349.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     SEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHYGMIDAIDD
                     PQVFAALFGAAEAWLKSQGANKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1208..1972)
                     /gene="glcC"
                     /locus_tag="ACHOQV_004064"
     CDS             complement(1208..1972)
                     /gene="glcC"
                     /locus_tag="ACHOQV_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFP9632350.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDASPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2223..3722
                     /gene="glcD"
                     /locus_tag="ACHOQV_004065"
     CDS             2223..3722
                     /gene="glcD"
                     /locus_tag="ACHOQV_004065"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFP9632351.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3722..4774
                     /gene="glcE"
                     /locus_tag="ACHOQV_004066"
     CDS             3722..4774
                     /gene="glcE"
                     /locus_tag="ACHOQV_004066"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFP9632352.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4785..6008
                     /gene="glcF"
                     /locus_tag="ACHOQV_004067"
     CDS             4785..6008
                     /gene="glcF"
                     /locus_tag="ACHOQV_004067"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFP9632353.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVTPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGVEAILQTASGCGAFVKEYGQMLKNDALYADKARLVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6013..6417
                     /locus_tag="ACHOQV_004068"
     CDS             6013..6417
                     /locus_tag="ACHOQV_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFP9632354.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6439..8610
                     /gene="glcB"
                     /locus_tag="ACHOQV_004069"
     CDS             6439..8610
                     /gene="glcB"
                     /locus_tag="ACHOQV_004069"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFP9632355.1"
                     /translation="MSQTITQGRLRINANFKRFVDEEVLPGTGLDTAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPSCILLKNNGLHIELQIDANGRIGKDDSAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPHEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKDQVQASLENMAKVVDQQNAGDPAYRPMVE
                     NFANSCAFKAACDLIFLGVKQPNGYTEPLLHAWRLREKENH"
     gene            8966..10648
                     /gene="glcA"
                     /locus_tag="ACHOQV_004070"
     CDS             8966..10648
                     /gene="glcA"
                     /locus_tag="ACHOQV_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFP9632356.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFDIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLSLFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYRLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
     gene            complement(10676..>10956)
                     /locus_tag="ACHOQV_004071"
     CDS             complement(10676..>10956)
                     /locus_tag="ACHOQV_004071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632357.1"
                     /translation="FNKQKSNNYSQCNYPKLLIVLTIAIAKATDSTVFAHQANIISTN
                     LFCVIKNRYSHVLNYLAGYLNAKHYCCMDNKEGNNFQLIRQNNVNVAG"
BASE COUNT         2551 a         2737 c         3070 g         2598 t
ORIGIN      
        1 ccgttcgttg ctgttgcaac ctgtttttca ccgttaatta tttgttatcc acagagatat
       61 ggaatgcgat ctgctgatga ttaaaattga aaaagtaatt aataaaaatg accttaaggc
      121 atttatcgct tttccgtcat cactttatcc cgatgatcca aactggatac cccctttatt
      181 cattgaacgc agcgagcatt tgtctgcgaa gaatccgggg acggaccata tcatctggca
      241 ggcgtgggtg gcaaaaaaag cggggcagat agttgggcgt attaccgcgc aaatcgatac
      301 cttgcaccgt gagcgttacg gtgaagatac cggtcattac ggaatgattg atgccattga
      361 tgatccgcag gtttttgccg cgttgtttgg cgcagcggaa gcgtggttaa agtcacaagg
      421 tgcaaataag atcagcgggc ctttcagcct gaatatcaat caggaaagcg gattactgat
      481 tgaaggtttt gacactccgc cctgtgcgat gatgccacac ggcaaaccgt ggtatgccgc
      541 gcatattgaa caactgggtt atcacaaagg cattgattta ctggcgtggt ggatgcagcg
      601 aaccgatctc acgttctctc cggcgctaaa aaaactgatg gatcaggtgc gcaaaaaagt
      661 gaccattcgc tgcatcaatc gccagcggtt tgccgaagag atgcagatcc tgcgtgagat
      721 tttcaattct ggctggcagc acaactgggg atttgtgccg tttaccgaac atgaattcgc
      781 gaccatgggc gatcaactta aatatctggt gccggacgat atgatctaca tcgctgagat
      841 tgattctgca ccctgcgcgt ttattgttgg cttgccgaac atcaacgagg cgattgccga
      901 tctgaacgga tcgctctttc ccttcggctg ggcaaaattg ctgtggcgtt tgaaagtcag
      961 cggtgtgcga actgcgcgag tgccgctgat gggcgtgcgg gacgagtatc agttcagccg
     1021 catcggcccg gtaattgcgc tgttattgat tgaagcctta cgcgatccgt ttgcccgtcg
     1081 gaagatcgat gcgctggaga tgtcatggat cctcgaaacc aatacaggca tgaataacat
     1141 gctggaacgc attggggcgg agccgtacaa gcgttaccga ttgtatgaaa agcagatttg
     1201 atggcagtca cccctgcttc atctccagcg gcaccgccga gcgaatcaaa tggtggcctt
     1261 ccagctcgat atcgtggaga ttctttttca cggtccgcac atgatcacgt gctgcgcgct
     1321 gggcgacgtg cggcagccgc tgcaacaccg cgttgtagat ccgcgcatgc tggcgatcga
     1381 tctgcttttt ttgctgtggt cgatggtaga gattatttac gctggcaaac actgaattaa
     1441 acatcagatc ggtcaatgat tgcagcgtaa acaccagcac ctgattgtga gaagcctgac
     1501 aaatggcgag atggaaagcg tgatccaact gcgcatgttc gatcagcgaa atctctttgt
     1561 tgttctcact ggcggcgagc attttttcat aacagcgggt tatcacaaca aaatcagcct
     1621 gggttcccag cgttgccgcc agccttgccg attcgccctc cagtaatgcg cgaacgtcga
     1681 gcagatcgta cagcgttcgc ggctgcgtac tgaacagatg gatcagcggg ctggcgtcct
     1741 gcacccgatt aagccgtgcg acacgagaat cgcgaccctg cgccgtttca ataatcccgc
     1801 gcccgcgcag cacggtcagc ccttcacgca gtgcggagcg tgagaagccg agcttttcac
     1861 acagtcgacg ttccgaggga agcggctgac cgaccttcaa cacgccgtcg ataattaacc
     1921 gttcgatact ctctgcaacc acttcgcaaa tagggcgacg ttcatctttc attcccggac
     1981 ctcgtgcaca ggtaggacca atttttctgt tcttaactcg caaaacacgc acatcacgca
     2041 agtgattatg ttacatcaat ttaacattaa gttaaccaag gcaaggtcac agagctggaa
     2101 aaaaaatggt ctgaccggta gctaaagaga tagacgaaaa cgaaaagccc gcttaataac
     2161 tgttcacaga agcagcgcgc aaaaatcagc tgccacacaa cacaacaaag cgaagcctat
     2221 tcatgagcat cttgtacgaa gagcgtcttg atggcgcttt acccgatgtc gaccgcacat
     2281 cggtactgat ggcactgcgt gagcatgtcc ctggacttga gatcctgcat accgatgagg
     2341 agatcattcc ttacgagtgt gacgggttga gcgcgtatcg cacgcgtcca ttactggttg
     2401 ttttgccaaa gcagatggaa caggtgacgg cgattctggc tgtctgccac cgcctgcgtg
     2461 taccggtggt gacccgtggt gcaggcaccg ggctttctgg tggcgcgctg ccactggaaa
     2521 aaggtgtgtt gttggtgatg gcgcgcttta aagagatcct cgacattaac cccgttggtc
     2581 gccgcgcgcg cgtgcagccg ggcgtgcgta atctggcgat ctcccaggcc gttgcgccgc
     2641 ataatctcta ttacgcgccg gatccttcct cacaaatcgc ctgttcaatt ggcggcaatg
     2701 tggcggaaaa tgccggtggc gttcactgcc tgaaatatgg tctgaccgta cataacctgc
     2761 tgaaaattga agtgcaaacg ctggacggcg aggcgctgac gctgggatcg gatgcgctgg
     2821 attcgcctgg ttttgacctg ctggcgctgt tcaccggatc ggaaggtatg ctcggcgtga
     2881 ccaccgaagt gacggtaaaa ctgctgccga agccgcccgt ggcgcgggtt ctgttagcca
     2941 gctttgactc ggtagaaaaa gccggacttg cggttggtga catcatcgcc aatggcatta
     3001 tccccggcgg gctggagatg atggataacc tgtcgatccg cgcagcggaa gattttattc
     3061 atgccggtta tcccgtcgac gccgaagcga ttttgttatg cgagctggac ggcgtggagt
     3121 ctgacgtaca ggaagactgc gagcgggtta acgacatctt gttgaaagcg ggcgcgactg
     3181 acgtccgtct ggcacaggac gaagcagagc gcgtacgttt ctgggccggt cgcaaaaatg
     3241 cgttcccggc ggtaggacgt atctccccgg attactactg catggatggc accatcccgc
     3301 gccgcgccct gcctggcgta ctggaaggca ttgcccgttt atcgcagcaa tatgatttac
     3361 gcgttgccaa cgtctttcat gccggagacg gcaacatgca cccgttaatc cttttcgatg
     3421 ccaacgaacc cggtgaattt gcccgcgcgg aagagctggg cgggaagatc ctcgaactct
     3481 gcgttgaagt tggcggcagc atcagtgggg aacatggcat cgggcgcgaa aaaatcaatc
     3541 aaatgtgcgc ccagttcaac agcgatgaaa tcacgacctt ccatgcggtc aaggcggcgt
     3601 ttgatcccga tggtttgctg aaccctggga aaaacattcc cacgctacac cgctgtgctg
     3661 aatttggtgc catgcatgtg catcacggtc atttaccttt ccctgaactg gagcgtttct
     3721 gatgctacgc gagtgtgatt acagtcaggc gctgctggag caggtgaatc aggcgattag
     3781 cgataaaacg ccgctggtga ttcagggaag caatagcaaa gcctttttag gccgccctgt
     3841 caccgggcaa acgctggatg tgcgttgtca tcgaggcatt gttaattacg acccgaccga
     3901 gctggtgata accgcgcgtg ccggaacgcc gctggtagcc attgaagcgg cgctggagag
     3961 cgccgggcaa atgctcccct gcgagccgcc gcattatggt gaagaagcca cctggggcgg
     4021 gatggtcgcc tgcgggctgg cggggccgcg tcgcccgtgg agcggttcgg tccgcgattt
     4081 tgtcctcggc acgcgcatca ttaccggcac tggaaaacat ctgcgttttg gtggcgaagt
     4141 gatgaaaaac gttgccggat acgatctctc acggttaatg gccggaagct acggttgtct
     4201 gggtgtgctc actgaaatct cgatgaaagt gttaccgcga ccacgcgcca ccctgagtct
     4261 gcgtcgggaa atcagcctgc aagaagccat gaatgaaatc gcccagtggc aacttcagcc
     4321 attacccatt agtggcttat gttacttcga caatgcgttg tggatccgcc ttgagggcgg
     4381 cgaaggatcg gtaaaagcag cgcgtgaact gctggggggc gaagaggttg cgggtcagtt
     4441 ctggcagcaa ttgcgtgaac aacaactgcc gttcttctcg ttaccaggta ccttatggcg
     4501 catttcatta cccagcgatg cgccgatgat ggatttaccc ggcgagcaac tgatcgactg
     4561 gggcggggcg ttacgctggc tgaaatcgac agccgatgac aatcaaatcc atcgcatcgc
     4621 ccgcaacgct ggcggtcatg cgacccgctt tagtgccgga gatggtggct ttgccccgct
     4681 accggctcct ttattccgct atcaccagca gcttaaacag cagctcgacc cttgcggcgt
     4741 gtttaacccc ggtcgcatgt acgcggaact ttgaggagca ggctatgcaa acccgattaa
     4801 cagaagagat gcggcagaac gcgcgcgcgc tggaagccga cagcatcctg cgcgcctgtg
     4861 ttcactgcgg attttgtacc gcaacctgcc caacctatca gcttctgggc gatgaactgg
     4921 acgggccgcg cgggcgcatc tatctgatta aacaggtact ggaaggcaac gaagtcacgc
     4981 ttaaaacaca ggagcatctc gatcgctgtc tcacttgccg caattgtgaa accacctgtc
     5041 cttctggtgt gcgctatcac aatttgctgg atatcgggcg tgatattgtc gagcagaaag
     5101 tgaaacgccc actgccggag cgaatgctgc gcgaaggatt gcgccaggta gtgccgcgtc
     5161 cggcggtctt ccgtgcgctg acgcaggtag ggctggtgct gcgaccgttt ttaccggaac
     5221 aggtcagagc aaaactgcct gctgaaacgg tgaaagctaa accgcgtccg ccgctgcgcc
     5281 ataagcgtcg ggttttaatg ctggaaggct gcgcccagcc tacgctttcg cccaacacca
     5341 acgcggcaac tgcgcgagtg ctggatcgtc tggggatcag cgtgacgcca gctaacgaag
     5401 caggctgttg tggcgcggtg gactatcatc ttaatgcgca agagaaaggg ctggcgcggg
     5461 cgcgcaataa tattgatgcc tggtggcccg cgattgaagc aggtgtcgag gcaattttgc
     5521 aaaccgccag cggttgcggc gcgttcgtta aagagtatgg gcagatgctg aaaaacgatg
     5581 cgttgtatgc cgataaagcg cgtctggtca gtgaactggc ggtcgattta gtcgaacttc
     5641 tgcgcgagga accgctggaa aaactggcaa ttcgcggcga taaaaaactg gccttccact
     5701 gcccgtgtac cctacaacat gcgcaaaagc tgaacggcga agtggaaaaa gtgttacttc
     5761 gtcttggatt taccttaacg gacgttcccg acagccatct gtgctgcggt tcagcgggaa
     5821 catatgcgtt aacgcatccc gatctggcac gccagctgcg ggataacaaa atgaatgcgc
     5881 tggaaagcgg caaaccggaa atgatcgtca ccgccaacat tggttgccag acgcatctgg
     5941 cgagcgccgg tcgtacctct gtgcgtcact ggattgaaat tgtagaacaa gcccttgaaa
     6001 aggaataaca aaatgaaaac taaagtcatt cttagccagc aaatggcgag tgcaattatt
     6061 gccgcaggtc aggaagaggc gcagaaaaat aactggtctg tttccattgc tgttgccgat
     6121 gacggcggtc atctgctggc gttaagtcgc atggacgatt gcgcgccgat tgcggcttac
     6181 atctcccagg agaaagcgcg taccgccgcg ctggggcgtc gtgaaactaa gggctatgaa
     6241 gagatggtga acaacggacg taccgcgttc gtgactgcgc cgttactgac gtcgctggaa
     6301 ggtggcgtac cggttgttgt ggatgggcaa attattggtg ccgtgggcgt ttctggttta
     6361 accggagcac aggatgcgca ggtcgcgaaa gcggcagcag cggtgttggc gaaataagcg
     6421 aaaacgagga gataaacaat gagtcaaacc ataacccagg gccgtttacg cattaacgcc
     6481 aattttaaac gttttgtgga tgaagaagtt ttaccgggaa cagggttgga cactgcggcg
     6541 ttctggcgca attttgatga gatcgttcac gatctggcac cagaaaatcg tcagttgctg
     6601 gcagaacgcg atcgcattca ggcggcgctt gatgagtggc atcgcagcaa tccggggccg
     6661 gtaaaagata aagcggccta taaatctttc ctgcgtgaac tgggctacct ggtgccgcaa
     6721 ccggagcgcg tgacggtgga aaccacgggc attgacagcg aaatcaccag ccaggcgggg
     6781 ccacagctgg tggttccggc aatgaacgcc cgctacgcgc tgaacgcggc gaacgctcgc
     6841 tggggctcac tgtacgatgc gttatacggc agcgacatca tcccgcagga aggggcgatg
     6901 gtcagcggct acgatccgca acgcggtgag caggttatcg cctgggttcg gcgtttcctc
     6961 gatgaatcac taccgctgga aaacggcagc tatcaggatg tggtggcgtt taaggtggtc
     7021 gataaacaat tacgcatcca gttgaaaaat ggtaaagaaa ccacgttacg taccccggcg
     7081 cagtttgtcg gttaccgtgg cgatgccgct gcgccgtcct gcattttgct gaaaaataac
     7141 ggcctgcata ttgagctaca aatcgatgcc aacgggcgga ttggcaaaga cgattcggcg
     7201 cacatcaacg atgttatcgt cgaagcggcc atcagtacca ttctcgactg cgaagattcg
     7261 gttgcggcgg ttgatgcgga agataaaatc ctgctgtacc gcaatctgct gggtctgatg
     7321 caggggactc tgcaagagaa aatggagaaa aacggtcggc aaatcgtgcg taaactgaat
     7381 gacgatcgtc agtacaccgc tgccgacggc tctgaaattt ctctgcacgg gcgttcgctg
     7441 ctgtttatcc gcaacgtggg tcatttgatg accattcctg tgatttggga cagcgaaggc
     7501 aatgaaatcc cggaaggcat tcttgatggc gtcatgactg gcgcgattgc cctctatgat
     7561 ttaaaagtgc agaaaaactc gcgcactggc agcgtctata ttgtgaaacc gaaaatgcac
     7621 ggtccgcatg aagtggcgtt cgccaacaaa ctgtttaccc gcattgagac aatgctcggt
     7681 atggcaccga ataccctgaa aatgggcatt atggatgaag aacgtcggac ctcgctgaac
     7741 ttgcgtagct gtatcgctca ggcgcgtaac cgcgtggcgt tcatcaatac cggtttcctc
     7801 gaccgtaccg gcgatgaaat gcattcggtg atggaagctg gcccgatgct gcgtaaaaac
     7861 cagatgaaat cgacgccgtg gatcaaagcc tacgaacgta ataacgtgct ttccggtctg
     7921 ttctgtgggc tgcgcggtaa agcgcaaatc ggtaaaggca tgtgggcaat gccggacctg
     7981 atggcagaca tgtacagcca gaagggcgac cagctgcgtg ccggggcaaa cacggcctgg
     8041 gttccgtcgc caactgccgc tacgctccat gcgctgcact accaccaaac caacgtacag
     8101 agcgtgcaag ccaacattgc ccagaccgag ttcaatgctg aatttgaacc gctgctggac
     8161 gatctgctga ctattccggt tgctgaaaac gctaattggt cggcgcaaga gatccaacaa
     8221 gagctggata acaacgtgca ggggattctg gggtacgtgg tgcgttgggt ggagcagggg
     8281 attggttgtt caaaagtgcc ggatattcac aacgtggcgt tgatggaaga ccgcgcaacg
     8341 ctgcgtattt ccagccagca tatcgccaac tggttacgtc acggtattct gaccaaagat
     8401 caggtgcagg cgtcgctgga gaatatggcg aaagtggttg atcagcaaaa cgctggcgat
     8461 ccggcttatc gtccaatggt ggagaatttc gctaactcgt gtgcttttaa agctgcctgc
     8521 gatttgatct tcctcggagt gaaacagcca aacggctata ccgaaccgtt attacacgca
     8581 tggcgtttac gcgaaaaaga aaatcattaa tagcgtcaaa aaaagccccg gcaagattta
     8641 ttgtcggggc tatgatagta actgcggtag tacatttatc ccggaaataa ccggagcgat
     8701 tatcaggggt taacaatatt aaatacccgt agcgcatcac tagcgggatt aaaacaacaa
     8761 tatggacatc ttaacgatac tctgcgcctg cgtgtgcgga acagaaaccc ttacccgata
     8821 tacttgttat gccagaggta gcaattgcgt tagcggcgta ctgttgctgt aaagtataac
     8881 gcctatattt tacagttcag tcctcttttg cttattacaa ccttaagtat atcaagcata
     8941 taaagataat aagagactga acaatatggt tacctggacc caaatgtata tgccgatggg
     9001 aggactgggg ctatccgctc tggtcgccct gatcccaata atattcttct tcgttgcact
     9061 cgcggtatta cgtctgaaag gacatgtcgc tggagcaata acccttatat tatctattct
     9121 gattgcgata ttcgccttta aaatgccgat tgatatggca tttgctgctg cgggctatgg
     9181 ctttatttat ggtttatggc ctattgcgtg gattattgtc gcggcggtat tcctgtataa
     9241 attaaccgtt gccagcggcc agttcgatat tatccgcagc tcggttatct ccatcaccga
     9301 cgatcagcgt ttgcaggtgt tactgattgg tttctccttt ggtgcgctgc tggaaggggc
     9361 ggctggcttt ggcgcaccgg tggcgattac cggtgctcta ctggtgggcc tgggcttcaa
     9421 accgctatac gcggcggggc tgtgtctgat tgccaacact gcgccggtgg cgtttggtgc
     9481 gttgggtgtg ccaattctgg tcgccggtca ggtaacggga atcgatccgt tcgacattgg
     9541 cgcaatggcg ggacgtcagt taccgttcct gtcggttctt gtgccgttct ggctggtagc
     9601 aatgatggac ggctggaaag gggtgaaaga gacgtggcca gcggcgctgg ttgcaggggg
     9661 aagcttcgct gtcactcagt tctttacctc taactatatt ggtccggaac tgccggatat
     9721 tacttcggcg ctggtgagta tcgtctcact ttctttattt cttaaagtct ggcggccaaa
     9781 aaataccgaa acggcaatca gtatgggaca atccgcaggt gcgatggtgg tgaataaacc
     9841 gtcttctggc ggtcccgtgc cttcagaata tcgtctgggg caaatcattc gtgcgtggtc
     9901 accgttttta atcttaacgg tgttggtcac catctggacc atgaagccgt ttaaagcgtt
     9961 atttgctccg ggcggcgcgt tttattcgct ggtgattaat ttccagatcc ctcatttgca
    10021 tcaacaagtg ttgaaagcgg cccccattgt cgcccagccg acgccaatgg acgcggtgtt
    10081 taaattcgac cccctctctg ctggtggcac cgctattttt attgcggcga ttatctcgat
    10141 cttcatcctc ggcgtgggga tcaagaaagg tattggtgtc tttgccgaaa cgctaattag
    10201 tctgaagtgg ccgatactgt cgattggcat ggtgctggca tttgccttcg tgaccaacta
    10261 ttccggcatg tccaccacgc tggcgctggt actggctggt acaggcgtaa tgttcccgtt
    10321 tttctcaccg tttctcggct ggctgggcgt attccttacc ggctcggaca cctcctctaa
    10381 cgccctgttt ggttcactgc aatcgaccac ggcgcagcaa atcaacgtct ctgacaccct
    10441 gctggtggca gcaaatacca gcggcggcgt aactggcaag atgatctccc cgcaatctat
    10501 cgccgtggcc tgcgccgcga cgggcatggt gggccgagaa tctgaactgt tccgctacac
    10561 cgtgaagcac agtctgattt ttgccagcgt tatcggcatt atcacgctgt tgcaggcgta
    10621 tgtgtttacc gggatgttag tttcgtaagt caataatgcc ggatgatcgt taatatcatc
    10681 cggctacatt tacattattc tgtcttatta attgaaaatt atttccctct ttattatcca
    10741 tgcagcaata atgttttgcg ttcagataac ctgctaaata attaagaacg tgagaatatc
    10801 tattttttat cacgcaaaac aagtttgtac taattatgtt cgcttgatga gcaaacaccg
    10861 tgctatctgt tgcttttgca atcgcaatcg ttaaaactat caacaacttg ggatagttac
    10921 attgtgaata attgttactt ttttgtttgt tgaatc
//
LOCUS       DAZKGF010000165        10901 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZKGF010000165 DAZKGF010000000
VERSION     DAZKGF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.165"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            80..184
                     /locus_tag="ACHOQV_004072"
     CDS             80..184
                     /locus_tag="ACHOQV_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632358.1"
                     /translation="MENNSRTMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            442..2640
                     /gene="speF"
                     /locus_tag="ACHOQV_004073"
     CDS             442..2640
                     /gene="speF"
                     /locus_tag="ACHOQV_004073"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFP9632359.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINENSNIDVAAIVLSLNDVT
                     RGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEFAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLSEVLPSIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2637..3956
                     /gene="potE"
                     /locus_tag="ACHOQV_004074"
     CDS             2637..3956
                     /gene="potE"
                     /locus_tag="ACHOQV_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFP9632360.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSADEGYFPKIFSRVSKVDAPVQGMLTIVIIQS
                     GLSLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4013..5653)
                     /gene="pgm"
                     /locus_tag="ACHOQV_004075"
     CDS             complement(4013..5653)
                     /gene="pgm"
                     /locus_tag="ACHOQV_004075"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFP9632361.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5679..6224)
                     /gene="seqA"
                     /locus_tag="ACHOQV_004076"
     CDS             complement(5679..6224)
                     /gene="seqA"
                     /locus_tag="ACHOQV_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFP9632362.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6409..7173
                     /gene="ybfF"
                     /locus_tag="ACHOQV_004077"
     CDS             6409..7173
                     /gene="ybfF"
                     /locus_tag="ACHOQV_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9632363.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7313..7606
                     /gene="ybfE"
                     /locus_tag="ACHOQV_004078"
     CDS             7313..7606
                     /gene="ybfE"
                     /locus_tag="ACHOQV_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFP9632364.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7746..8276
                     /gene="fldA"
                     /locus_tag="ACHOQV_004079"
     CDS             7746..8276
                     /gene="fldA"
                     /locus_tag="ACHOQV_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFP9632365.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8486..8572
                     /gene="uof"
                     /locus_tag="ACHOQV_004080"
     CDS             8486..8572
                     /gene="uof"
                     /locus_tag="ACHOQV_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFP9632366.1"
                     /translation="MIRIISRANSVTSPNEVNRLVTGQIPHD"
     gene            8565..9011
                     /gene="fur"
                     /locus_tag="ACHOQV_004081"
     CDS             8565..9011
                     /gene="fur"
                     /locus_tag="ACHOQV_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFP9632367.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9095..9421)
                     /gene="chiQ"
                     /locus_tag="ACHOQV_004082"
     CDS             complement(9095..9421)
                     /gene="chiQ"
                     /locus_tag="ACHOQV_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFP9632368.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9471..10877)
                     /gene="chiP"
                     /locus_tag="ACHOQV_004083"
     CDS             complement(9471..10877)
                     /gene="chiP"
                     /locus_tag="ACHOQV_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFP9632369.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDIYDGTAWLQALTFGYRAADVVDLRLEGSWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2626 a         2755 c         2701 g         2819 t
ORIGIN      
        1 attgatcacc ttcacagttc aaccgtattt cctggataga ataccttcac cttcattcac
       61 atcaggaaag gtaaattaaa tggaaaataa cagccgcact atgccccata taaggcggac
      121 aactcatatt atgaagtttg ctcatcgcaa tagcttcgac tttcacttct tcaatgcccg
      181 ttagtctacc gactaagggc acttcagcgt acaggtcttc ctgactctct gtattacagg
      241 catatgcctg tattccgcag tgctcattct ctgttaaccc ttgtattatc agccaccgga
      301 ctgataaggg tttctgcatt ctgaaaaaag caaacttctg attaatttat cagcggggta
      361 actttgcttt ttttccggca cgatcgattt ctcattcgag aaattgagga cctgctatta
      421 cccaaaataa agagatggaa aatgtcaaaa ttaaaaattg cggttagtga ttcttgcccg
      481 gactgtttta ccacgcagcg agaatgtatc tatattaatg aaaacagtaa tatcgatgtg
      541 gcggcaatag ttttatcgct caacgatgtt acacgcggaa aactcgatga aatcgatgcc
      601 acgggttatg gcatcccggt atttattgct actgaaaatc aagaacgtgt acccgcagag
      661 tatttgcccc gtatttcggg tgtctttgag aattgcgaat cgcgacgaga attttatggt
      721 cgtcagttag aaaccgcagc cagccattat gaaacgcaac tgcgcccgcc cttcttccgc
      781 gcactggtcg attatgtcaa tcagggtaac agcgcgtttg attgccccgg tcatcagggc
      841 ggcgaatttt tccgtcgcca tcccgcgggg aatcagtttg tggaatactt tggtgaggcg
      901 ctgttccgtg ctgacttgtg caacgccgac gtagcgatgg gcgatctgct gattcacgaa
      961 ggcgcgccat gcattgcaca gcaacatgcg gcaaaagtgt ttaatgccga taaaacatac
     1021 ttcgttttaa atggcacttc atcttctaac aaagtggttt taaacgccct gctgacaccg
     1081 ggtgatctgg tgctgtttga ccgcaataac cacaaatcta accaccacgg agcgttgcta
     1141 caggctggcg cgacaccggt ttatctggaa accgcacgta acccgtatgg ttttatcggt
     1201 ggtattgacg ctcactgttt tgaagaaagt tacctgcgtg aactgatcgc ggaattcgca
     1261 ccgcagcggg caaaagaggc tcgtcctttc cgcctcgccg ttattcagct aggcacttac
     1321 gacggtacga tttataacgc ccgtcaggtg gtggataaaa ttggtcatct gtgtgactac
     1381 atcctctttg actccgcatg ggtcggctat gagcagttta tcccgatgat ggcggactgt
     1441 tcaccactgt tgctggatct taatgagaac gatccgggta ttctggttac gcaatctgtg
     1501 cataaacagc aggctggttt ttcccagact tcacaaatcc ataaaaaaga cagccatatc
     1561 aaagggcaac agcgttatgt accgcacaaa cgcatgaaca acgcctttat gatgcatgcc
     1621 tccaccagcc cgttctatcc gctgtttgcc gcactggata tcaacgccaa aatgcatgaa
     1681 ggtgtcagcg gtcgtaatat gtggatggat tgtgtggtaa atggcattaa tgcccgcaaa
     1741 ctaatcctcg ataactgtca gcatattcgt ccattcgtac ctgaactggt ggatggtaaa
     1801 ccctggcagt cgtatgaaac ggcgcaaatt gcggttgatc tgcgcttctt ccagtttgta
     1861 ccaggtgagc actggcattc ttttgaaggc tatgcagaga atcaatactt tgtcgatccg
     1921 tgcaaactgt tgctgacaac cccaggtatt gatgcacgta acggcgaata tgaagcgttc
     1981 ggtgtacccg cgacgattct tgctaacttc ctgcgcgaaa atggcgtagt gccggaaaaa
     2041 tgcgatctta actccatcct ctttctgctg actccggcag aagatatggc caaacttcag
     2101 caacttgttg ctctgctggt acgcttcgaa aaactgcttg agtccgacgc gccattatca
     2161 gaagtactgc cttccatcta caaacagcat gaagagcgct acaccggtta taccctgcgt
     2221 cagttgtgtc aggaaatgca tgatttgtat gcccgtcaca acgtgaaaca actgcaaaaa
     2281 gagatgttcc gtaaggaaca cttcccacgc gtaagcatga atccacaaga agccaactac
     2341 gcctatttac gcggtgaagt ggagctggtt cgtctgccgg atgcagaagg ccgtatcgct
     2401 gccgaaggtg cgcttcctta tcctccgggt gtgctgtgtg ttgttccggg tgaaatctgg
     2461 ggtggtgctg ttctgcgtta cttcagcgct ctggaagaag ggatcaacct gctgccaggt
     2521 tttgcaccag agctgcaggg tgtctatatc gaagaacatg atggtcgtaa gcaagtttgg
     2581 tgctatgtca tcaagcctcg tgatgcgcaa agcaccctgt tgaaagggga aaaattatga
     2641 gtcaggctaa atcgaataag atgggcgtcg ttcagttaac catactgacg atggtcaaca
     2701 tgatgggctc cggtatcatc atgctgccga caaagcttgc cgaagttggg acaatctcaa
     2761 ttatctcctg gctggtgaca gccgtaggct caatggcact ggcatgggca ttcgcaaaat
     2821 gcggtatgtt cagccgtaaa tcaggcggta tgggcggtta tgcggagtat gctttcggta
     2881 aatccgggaa ctttatggcg aactacacct acggtgtctc gctgctgatt gctaacgtcg
     2941 cgattgctat ttcggcggtt ggttacggca ccgaactgct cggcgcgagt ttgtcgccag
     3001 tgcagattgg tcttgcgacc atcggagtgc tgtggatttg taccgtggct aactttggtg
     3061 gtgcgcgcat taccgggcaa atcagtagca ttactgtgtg gggggtcatt attccggtcg
     3121 tcggtctgtg cattatcggc tggttctggt ttagcccgac gctatacgct aactcatgga
     3181 acccgcacca tgtgccgttc tttagcgctg taggttcttc catcgccatg acgttgtggg
     3241 ctttcctcgg tctggagtca gcctgtgcca acgccgaagt agttgaaaac ccggaacgta
     3301 acgtgccgat tgcagttctt ggcggtacgt taggtgcggc ggtgatttat atcgtctcca
     3361 ccaacgtgat tgccgggatt gtgccaaata tggagctggc aaattcaacg gcaccatttg
     3421 gtctggcctt cgcgcagatg ttcacgccgg aagtgggtaa agtcattatg gcgctgatgg
     3481 tgatgtcctg ctgcggttcg ctgcttggct ggcagttcac cattgcccag gtgtttaaat
     3541 cttctgccga tgaagggtac ttcccgaaaa ttttctcccg tgtgagcaaa gtcgatgcac
     3601 cagtacaggg aatgctgacc attgtcatca tccagagcgg attatcactg atgaccatta
     3661 gcccgtcgct gaacagtcag ttcaacgtgc tggttaacct ggccgtggtg accaatatca
     3721 ttccgtatat tctgtcaatg gcggcattag tcattattca gaaggtcgct aatgtgcccc
     3781 cctcaaaagc gaaagttgca aactttgttg cttttgttgg cgcgatgtac agcttttatg
     3841 cgctgtactc atccggggaa gaagccatgc tatacggttc catcgtgacc ttcctcggtt
     3901 ggacactgta tggtctggtc tcaccacgct ttgaactgaa aaataaacac ggttaattaa
     3961 gcaaagcaaa aaagggcgat cttccgaccg ccctttttct tatttgatat gtttacgcgt
     4021 ttttcagaac ttcgctaaca atctcaaccg cttctttctc aatctgcttg cgatgttctt
     4081 caccaaggaa gctttcgcag tagatcttat atgcgtcttc cgtgcctgac ggacgcgcgg
     4141 cgaaccagcc gttgtcagtc atcactttca gaccgccgat agaagcgcca ttacccggtg
     4201 ccgccgtcag acgcgcggtg atcgggtcac ctgccagggt gctggcgctc accatttccg
     4261 gagacagctt agacaacgcc gctttttgtg cggaagtcgc tgctgcctgc aaacggttat
     4321 agctcggcgc accaaagcgt tttgccagtt cgttatagtg ttcctgcggg ttcttaccgg
     4381 tgacagcggt gatttccgcc gccagcagac acataatgat accgtcttta tcggtggacc
     4441 acggcgtgcc gtcgaaacgc aggaaggaag cgcctgcact ctcttcgccg ccaaagccga
     4501 agctgccatc gaacaaacca tcaacaaacc atttgaaacc taccggtact tctaccagct
     4561 tacggcccaa atcattgacc acacggtcga tcatcgcaga tgaaaccagc gttttaccga
     4621 cagcaacatc tttgccccac tgcggacgat gctggaacag gtagttgatc gccaccgcca
     4681 ggtagtggtt cggattcatc aaacctgccg gagtgacgat accgtgacgg tcataatccg
     4741 ggtcgttagc aaacgccaga tcgaacttat cacgcagtgc cagcaggccc gccatcgcac
     4801 actcggagga gcagtccata cggatcgcac cgtctttatc gaggtgcata aagcggaagg
     4861 tttgatcgac ctgatcgtta acgatggtca ggttgaggtt gtaatactcg ccaatgcgct
     4921 tccagtattc gataccggaa ccacccagcg gatcaacgcc cagcgtcagg cccgctttct
     4981 gaatcgccgc catatcaacg atatcggcca gcccttccac aaacggctgc accagatcct
     5041 gctctttcac atgaccggat gccatcgctt cgtcgaggga gatacgcttc acgcctttca
     5101 ggccatcagc cagcagtgcg ttggctctgt cttccaccac tttagtgacg ttggtatcag
     5161 ccgggccacc atttggcgga ttgtacttga taccaccatc ttccggcggg ttatgggacg
     5221 gtgtaatcac gataccgtct gccagtgggc cacctttttt attgtgaacc agaatggcat
     5281 tggaaatggc aggtgtcgga gtgaagccat tgttttcctg cacaatgaca tcaacgccgt
     5341 tcgctgccag cacttccagt acggaaatga acgcaggctc ggacagggcg tgagtatctt
     5401 tacccacata gcaagggcca gtgatgccgt ttttcgcacg ttcttcagca attgcctgag
     5461 cgatcgccag aatgtgcggc tcgttaaagc tgtggcgcgc tgcactgcca cggtgaccgg
     5521 aagtaccgaa tttcaccgcg tgctccgcat tccctgcttc tggtttcagt acataatatt
     5581 gcgccgtcag ttgggcgacg ttaatcaaat cactctgttg tgcaggttgg cctgcacgat
     5641 tgtggattgc cattgctttg tcctttgtct gcaacgtttt agatagttcc gcaaaccttc
     5701 tcaatcaatt ccgccgggaa ttgcatcgac tgcatgatgt gttcgatcat gctgcattta
     5761 cggccggtgt tggtgttggt gatcacccaa tacggcgtac caggcacatg tttcggcttg
     5821 gtctgattac catttttcag cagcgtttgt tcatctgccg caaagtaaac gcgtgtacga
     5881 ccgtgcaacg attccgttgc ttcggcaaac gcctgggcgt caagagaata tagtgtagac
     5941 aacagcagca taaagcgatt gaccgctcgc ttttgctctg cgtattcgtc cgaaagcaga
     6001 agttcacgca ttgcgcgaac cttgtcttta atcgttttga ccggcttcgc ttcgacgata
     6061 gcaggtgacg caacgcgaac ctctttcgtc accggagcag caggctgtga tgcggcggaa
     6121 aatttcaaca tacgccgtaa aatgtcggat gcgctctcgc cgatatgctt agtgtggctg
     6181 gcaatatagc tgtagagttc atcatcaact tcaatcgttt tcatcttaat ccagtgcagt
     6241 gtcttagctg aatacaagtc gccaagagta tatgaataaa tcccggcagc ggatagcgtc
     6301 aagcctggct tgcagaaaaa ctcggaataa accttatttg ttgcagccct gtggcacaat
     6361 ggtcaacata ataccctaac ccgacaacgc gaaaaaaaga actttgccat gaaattgaat
     6421 atccgcgcgc aaactgcaca aaaccagcac aataattctc ccatcgttct tgtccatggt
     6481 ctgtttggca gcctcgacaa ccttggcgta ctggctcgcg atctggtaaa cgatcacaat
     6541 atcatccagg ttgatatgcg taaccacggt ctttcaccga gagatccggt aatgaattac
     6601 ccggcgatgg cgcaggatct tgttgatacc ctggatgcac agcagatcga caaagcaaca
     6661 tttatcggtc actccatggg cggtaaagcg gtaatggctc ttactgcact agcccccgat
     6721 cgcattgaca agctggtggc gattgatatt gcgccggtcg attatcacgt tcgccgtcat
     6781 gatgagattt tcgcggccat caacgcagtc agtgaatcag acgcacaaac acgccagcaa
     6841 gcagcggcaa taatgcgcca gcatcttaat gaagaagggg tgattcagtt tctgttgaaa
     6901 tcttttgttg acggggagtg gcgctttaac gtgccagtat tgtgggatca gtatccgcat
     6961 attgtgggtt gggagaaaat cccggcatgg gatcatccta ccctgtttat ccctggcggc
     7021 aattctccgt atgttagcga gcagtaccgt gatgatttac tggcacaatt tccacaggct
     7081 cgggcgcatg tgattgcagg cgcgggtcac tgggtccatg ctgaaaaacc ggatgcggta
     7141 ttacgcgcta tccgtcgcta tctcaatgat taacgcaact gattaaaaac ccagcgtccg
     7201 cgtcgctttc cgcgcgtgga cgttggcgtg actcatcgcg ctgatgtatt atggcgcgct
     7261 atccatccgg gcaggcgcct ggctgattgt ttcccccgaa gtcaccaaga tcatggccaa
     7321 agaacaaacg gaccgtacga cattagatct gttcgcgcac gagcgtcgac cgggacgacc
     7381 gaaaactaat ccgctttcgc gcgatgagca gctgcgtatt aataaacgca accaactaaa
     7441 acgcgacaaa gtacgtggcc ttaagcgtgt cgaactgaag ctgaacgcgg aagctgtcga
     7501 ggcactgaac gagctggcgg aatcgcgcaa tatgagccgt agcgagctga tcgaagagat
     7561 gttaatgcag caactggcgg cgctgcgtag tcagggcatc gtttaaattt ccactttcat
     7621 gtagcacagt gtgcagtcct gctcgtttgc tgattccgca agactgcctg ttctgctatg
     7681 attgccttta tccgtgggca attttccacc cccatttcaa taagtttcaa gaggttattt
     7741 cactcatggc tatcactggc atctttttcg gcagcgacac cggtaatacc gaaaatatcg
     7801 caaaaatgat tcaaaaacag cttggtaaag acgttgccga tgtccatgac attgcaaaaa
     7861 gcagcaaaga agatctggaa gcatatgaca ttctgctgct gggcatccca acctggtatt
     7921 acggcgaagc gcagtgtgac tgggatgact tcttcccgac tctcgaagag attgatttca
     7981 atggtaaact ggttgcgctg tttggttgtg gcgaccagga agattacgca gaatacttct
     8041 gcgatgcatt aggcaccatc cgcgacatca ttgaaccgcg cggcgcaact atcgttggtc
     8101 actggccaac cgcgggctac cacttcgaag cgtcaaaagg tctggctgat gatgaccact
     8161 ttgtcggtct ggctatcgac gaagatcgtc agccggaact gactgctgaa cgtgtagaaa
     8221 aatgggttaa acaaatttct gaagagctgc atctcgacga aattctcaat gcctgatgtg
     8281 atgcggcgta gactcatgtc tacgccgtat taatagataa tgccaatcaa aataattgct
     8341 acaaatttgt aacttttgct gttgtacctg tacaatgtcc cggtgttcaa gtggccttgc
     8401 cgttgtaaat gtaagctgtg ccacgttttt attaacaata tttgccaggg acttgtggtt
     8461 ttcatttagg cgtggcaatt ctataatgat acgcattatc tcaagagcaa attctgtcac
     8521 ttctcctaat gaagtgaacc gcttagtaac aggacagatt ccgcatgact gataacaata
     8581 ccgccctaaa gaaagctggc ctgaaagtaa cgcttcctcg tttaaaaatc ctggaagttc
     8641 ttcaggagcc ggacaaccat cacgtcagtg cggaagattt atacaaacgt ctgatcgata
     8701 tgggtgaaga aattggtctg gctacggtat atcgcgtact gaaccagttt gacgatgctg
     8761 gtatcgtcac ccgccacaat tttgaaggcg gtaaatccgt atttgaactg acacagcaac
     8821 atcaccacga tcacctgatc tgcctcgact gcggcaaggt tatcgaattt agtgatgatt
     8881 ccatcgaagc gcgtcagcgt gaaatcgccg caaaacatgg cattcgcctg actaaccaca
     8941 gtctctatct ttacggtcac tgtgccgaag gcgattgccg cgaagatgag cacgcgcacg
     9001 aaggcaaata agccagcctg aacgagaaaa gccaacctgc aggttggctt ttttatacaa
     9061 gggaaataag aaaagcgaga gttacagctc tcacttattt gttattactg cgaatttcct
     9121 gccagacctt atcacaatcg gctttcaccg cctgatcatt tccggtttgc gttgcctgaa
     9181 tacactgctg ataatccagt acgcggacac tttcctgctc ggcaaactgc tgatgttgtt
     9241 tctctttctt cagcacgttt aacacgctct ggcaggcttc aattttttcc ggcgaacctt
     9301 gcgcggtgtt gatacaggcg ctgtaagcct ctttcagacg actgtcttcc tgcggtgcgg
     9361 ttgattgcgc acaagctacc agccccgatg ccatcatggc gatgagaatc agttttttca
     9421 tagcaaattc ctcaaatgtg cggcaggcac aaaacctgcc gcgtcgggca tcagaagatg
     9481 gtgaatggtg cgattaccat aaatttcacg tcgcgctcat cctggaagat gttaccgtaa
     9541 ccgccgcccc agcttgggat gtcggagtgg ttgtcgtatt cggtgaagtg cagtttgaac
     9601 atcgtgcctt tggcgcgacc gtcctgaatg gtgtacaccg catccaggct gtaggcagac
     9661 tcttcaatag tccggttttt gtcgtagtac gcatccggat tgctctgcca ggtcgcaggt
     9721 ttagcatccc atgcgtaaac gtaggaagcg ccgatggcga agcctggaag attccagttt
     9781 ttcaggtcat acatcgcacc gaagaagacc gctttttcgc cgttggcgtt gaagtcagaa
     9841 cggttatccc accagatatc caggcgaccg ttcgaggaag cgtaggttgg agtcatacgt
     9901 tgcaggaagt atccctgctg accgtcggct ttaacccagc tgccttccag acgcaaatcc
     9961 actacatcag ccgcccggta accaaaggtc aacgcttgca gccaggcagt gccgtcgtaa
    10021 atgtcgttga cgctgcgatc gtcaacttta tcgcgcgtac cgtagaactg gtagctggtg
    10081 gttaacgggc taccggcgat atcaaatttg tagctggctt tggcaaaata ttgatctata
    10141 tacccttccg cctgaccaaa tgccgcttcc agtacaaagt tatttttgaa gtcgtacttc
    10201 gcaccgatgg agtgcagata atcaactttg gtagttttat cgttctgata aaactcatcc
    10261 atttcgagat gccacggtgc tttgtattcg ttggtccaca tgtaggagaa actcaacgca
    10321 ccagcatcgc cgtaatcaaa attcgccccg gcttccgcac cctgataagt acccggcata
    10381 aagctccagt gcggcgctaa cagcgtctga ccagttggct gaatgtaacc tgccctcgcc
    10441 cacaccggac cgtatttaaa tttggccgct gctttataca ggcttatacc gcttttgtcg
    10501 ccggaccagt cttcgtcata ggctttattg ctttttgaaa acgcgatttc gttcgggtga
    10561 gagctgtcac cgttttccgc catttcaatc gccgtaaacg cagcaatatc aaggccgaac
    10621 atatcagcag cataaccgga ctgaaaatcg aggttggcat tccaggtgga gtgagaaagg
    10681 ttggttttgt atttgtcgcc gtcggtaaca tctttacggt cgcgttcacg ctgccagtaa
    10741 tagataccgc cggttaaggt tgaatcgtcg atgaatcctt cggcgcgagc ctccggcatt
    10801 gccataaagc ccgacattgc tgtaacaccg gcgatagcca gcgccagcgt actacgtttg
    10861 ccactaaacg tacgcatggg ttaatcctct ttgacgtata a
//
LOCUS       DAZKGF010000166        10890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZKGF010000166 DAZKGF010000000
VERSION     DAZKGF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.166"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(43..1380)
                     /gene="adeP"
                     /locus_tag="ACHOQV_004084"
     CDS             complement(43..1380)
                     /gene="adeP"
                     /locus_tag="ACHOQV_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFP9632370.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            1547..2212
                     /gene="yieH"
                     /locus_tag="ACHOQV_004085"
     CDS             1547..2212
                     /gene="yieH"
                     /locus_tag="ACHOQV_004085"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFP9632371.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEDIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            2279..2752
                     /locus_tag="ACHOQV_004086"
     CDS             2279..2752
                     /locus_tag="ACHOQV_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFP9632372.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLMSITTGAIPAMLTGVMVACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVIV
                     HIKDMASSALFENILSGENLVVRIIPALLAGVVMSRIITHLPGLDISCPETDSLS"
     gene            complement(2834..3556)
                     /locus_tag="ACHOQV_004087"
     CDS             complement(2834..3556)
                     /locus_tag="ACHOQV_004087"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9632373.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLHNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLIVIADKAAAAELALG"
     gene            complement(3571..4740)
                     /locus_tag="ACHOQV_004088"
     CDS             complement(3571..4740)
                     /locus_tag="ACHOQV_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFP9632374.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPALKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNEPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(4767..6383)
                     /gene="bglH"
                     /locus_tag="ACHOQV_004089"
     CDS             complement(4767..6383)
                     /gene="bglH"
                     /locus_tag="ACHOQV_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFP9632375.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(6469..7863)
                     /gene="bglB"
                     /locus_tag="ACHOQV_004090"
     CDS             complement(6469..7863)
                     /gene="bglB"
                     /locus_tag="ACHOQV_004090"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFP9632376.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(7882..9756)
                     /gene="bglF"
                     /locus_tag="ACHOQV_004091"
     CDS             complement(7882..9756)
                     /gene="bglF"
                     /locus_tag="ACHOQV_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFP9632377.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKSQSAQVAQQEKTPEVITPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAI
                     MPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(9890..10726)
                     /gene="bglG"
                     /locus_tag="ACHOQV_004092"
     CDS             complement(9890..10726)
                     /gene="bglG"
                     /locus_tag="ACHOQV_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFP9632378.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIVKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRIIE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         2910 a         2777 c         2411 g         2792 t
ORIGIN      
        1 gccagcaatc atgcatggcg ggtttttaac gcgctatcga ttttagtgag cgtcgataaa
       61 gacaatcttc aggataaaca gcagcgcaac gatgattacg cacgggctaa ggtcacgcag
      121 acgtccggta ccaatcttca tcacgcagta ggagataaag cccagcgcga taccttcagt
      181 aatcgagaag ctgaacggca tcatcacagc ggtaataaac gccggaacag attcagtaag
      241 atcctgccag ttcacgcgag caagacttga ggtcatcaac acgccaacgt aaatcagcgc
      301 accagctgca gcgtagcctg gcaccatccc cgccagcggc gacaggaaga taaccagcag
      361 gaacagcaga ccaacaacca ctgccgtcag accggtacga ccgccaacgg aaacgccgga
      421 agaagattca atatacgccg taacagaaga agtaccgata aacgaaccgg tcaccgaaga
      481 gatactgtcg acatacagcg cctgcttcat gcgcgggaat ttacctttct catcagccag
      541 acctgcttta tcggtcacgc caatcagcgt accggaggag tcaaacaagt tgaccagcat
      601 gaaagagaaa atcacccctg ccagcccaag gttaaacgac ccggctaaat ctacatgacc
      661 cacgacactc attacgctcg gcggcgcaga aacgatgcca ttatagtgga catcacccag
      721 catccagccc agcagcgttg tcaccacgat ggaaaccagc accgccgcgt gaatattgcg
      781 cgaggccaga atggcgatga tgaagaagcc gaggatcccc agcagtacgc tgtgagaagt
      841 cagattaccg atgctcacca gcgtttccgg gttagcgaca atcacacctg cattcttcag
      901 tcccatcatg ccaataaaca ggccgatacc gctggtaatg cccacacgca gactcaccgg
      961 aatgttggct atcatccagt agcgaacgcg gaaaatcgtc agtaacagca gaccaatcgc
     1021 gccccagaag attgcgccca tcccgacctg ccacggcaag cccatcgcct gcacaaccac
     1081 aaaagcgaag aacgcattca ggcccatagc gggtgccagt gcaactggca ggttagcaaa
     1141 cagtcccatc ataatactgc cgaatgcagc aatcagacag gtagtaacga agacggcgct
     1201 ggtatccatg ccagcaacgc caagaatttg cgggttaaca aaaacgatgt aaaccatcgt
     1261 caggaaggtg gtaaaaccgg cgatcacttc ggtccgtgcc gtcgtgccat gttcgcgcag
     1321 tttaaacacg cgttccagca tcccctggcc agaagcctgg gttgtgtgtt gttgactcat
     1381 tatctatttc cgaacaagga gggaaaatcc gtcgctatcg tataccaaaa tgagacaata
     1441 tggacggctg aagataactt tttttcattg tatttgctta tacggcaacg attgcgtgat
     1501 gcgaattggc atgacgaaaa cgttaaacta acgataaagg aaagacatgt cccagataga
     1561 agcggtattt ttcgactgcg acggtacgct ggtcgatagc gaggtcattt gctctcgcgc
     1621 ctatgtcacg atgttcaggg aatttggcat tcacgttgac ctggaagata tttttacccg
     1681 ttttaaaggc gtaaaactct acgaaatcat cgacatcatc agtaaagaac agggcgtgac
     1741 gatggtccag gccgatgccg agcacgttta ccgcgcagaa gtagcacgtc tgtttgatac
     1801 cgaacttgag gacatcgcag gagcgaacga attgctggcg agcattaacg tgccgatgtg
     1861 cgtcgtctct aacggtccgg tcagcaaaat gcagcattcg cttggtaagc tcaacatgct
     1921 gcactacttc ccggaaaaac tgttcagcgg ctacgatatc cagcgttgga agcccgaccc
     1981 ggcgttaatg ttccatgcgg caaaagcgat gaatgtgaac gttgaaaact gcattctggt
     2041 cgatgactcc agcgccggtg cgcaatcagg aatcgatgct ggtatggaag tgttttactt
     2101 ctgcgctgac ccgcacaata agccgatcgt tcatccgaaa gtcaccacct ttacccatct
     2161 ttcgcaatta ccggaactgt ggaaggcgcg tggttgggat attacggcat agttattcac
     2221 actcccttca cttactccac ttaaattggc acgcaaaggt aagtaaaggg agtttgatat
     2281 gtctgtttca cgtcgggtaa ttcatcacgg actgtatttt gcggttttag ggccgttaat
     2341 tggtgttctg tttcttgtcc tctacatatt cttcgcaaaa gaaccgctga ttcttttggt
     2401 aataatacag gtacttccat tgtttctctt aatgtcgata actacgggag ctattcctgc
     2461 aatgttaact ggcgtaatgg tcgcctgtct gccggaaaag atcgggtcac agaaacgtta
     2521 tcgttgtctg gttggcggca taggtggagt cgttatcacc gagatctatt gcgctgttat
     2581 cgtacatatt aaggacatgg cttcctcagc gttgtttgaa aacattcttt ctggtgaaaa
     2641 tctcgttgtc cgcatcatcc cagcattgct ggcaggcgta gtgatgagca gaatcattac
     2701 acatctaccc ggattggata tttcatgtcc tgaaacagac tctttaagct aagcggaatg
     2761 ctttatctat ttgtcattaa atgttggaga acaggccgga gcgtaattca cacatccggc
     2821 cttattttta agcttaaccg agcgctaatt ctgcggcagc ggctttatca gcgatgacta
     2881 tcagcgaagg gtgcaactgc aatacggaag caggaacatc ttcggtaacc ggaccttgca
     2941 gaacattttt cagtgcctgc gctttacccg cgccactgac aattatcagc aagtttttcg
     3001 ccgccatgat gcttttcggc cccatcgtca cgtagctgtc aggcactaat gaaaagtcgc
     3061 cacccaactc gccatgcgcc acaatatcaa ccatctctcc ctggatcggg aactccacgg
     3121 tttgctcatg gaaatgggtc gtatttggca gattgccaca aaaatgacca tctacaccta
     3181 atcccaacac caccaaatcc aggccgcctt cacgcgccag tttctgatca tgctcgcggt
     3241 agttatcaat agtgagcttc tggatattct cttctttgat ccctgcaggg gtgaaaaaca
     3301 gattatgcag attggtaatc gttacgcctt ccccttcttt accgcgaaat ggaatttcat
     3361 cgaaattgta gaaatagcag ttatcgtacc agggcttacc tttaaccaga gtgatgagat
     3421 attcgtacat gcctttgggc gtgctcccgg cggtaattgc caggttaacg cgacgcatct
     3481 ttgacatata accaagcaga tgatgtgccg cgacacggct catttcctgg taatcttcgg
     3541 taatgatcaa tttcatctta atgccttata ttatttgaat aatttctgta caaaggctgc
     3601 ataggctggt cgccagacat ccatttcatg gttcagaccg ggatattcct ggtaatcaaa
     3661 cttaattttt ttctgctcaa gctcagtttt cagcccggcg atatccttgc cggttacgac
     3721 atctttatcg cccacgacca cagtaaaatt acgtagttgc tggttaatag ctgccggctc
     3781 gttcagccgg gcagcgaccc cttcatctgg tacggtcgtc gtggtaacac cactgaatgt
     3841 ggccagccag ccaaagcttt ccagatgatt cattcccgat accagtgcct gatacccacc
     3901 ttgtgaaagc cctgccagtg cgcgaccatc ggcatcttta cggacattaa aacgcttgct
     3961 aatcagcggg ataatatcgt tcatcaattc gcgatctgcc gctttagcat ttaacggata
     4021 aaagacttta cgtctctcct gaggaacgaa atcttcggga ataatgccct tcgcatcggt
     4081 ttcggtatcc gggatcacca ccagcatcgg tttaattttc ccttcagcca gcaggttatc
     4141 catgatttgc gggatacgcc cctgatcaat agcggaacgt ccggtatcac caaagccgtg
     4201 atagaaatag agcactggca aaggctcgcc tatgccggtg tatcctggcg gggtccagac
     4261 atacatctga cgttcggatt gcaatgcgtt ggagtggtaa gttatggcga tcaaatcacc
     4321 atgcacaaca gaacgtgtat ccagataact gcccggtacc agaatcatac tggagttaac
     4381 ctggcgctga ggcttagtca ttgctgtacc tgtatcaatg ctgcgtacac catcaacatt
     4441 gaaaaaatac tcgtacaggt tgcctttcag agcaggcgtg cgccacgacc agactcccgc
     4501 ttcgtctttg gtcatcgggt gaatattgtc cggaaccgga acacccacca ctacagagac
     4561 attttttgcc ccaggggcaa agtagcgaaa ggtcacgctg ttatcggtat tgacctgagt
     4621 cacatactgt ttcaccggaa tagtgggtgc cgggctggct ggcatatccg ctgctatggc
     4681 ccattgagca gaaataccgc tggcaatagc cagcgttaaa gcagcaattt ttatattcat
     4741 cgtaatattc cttttattaa gttccattac caccagattt cagcctgggc accgacagca
     4801 aactggtcgt ctttattgtc ttcaaaggtg aagccatcca gttcgttttc cagagctttg
     4861 atatatgtag cgtagaaacg aatttccggg cgcgaggtca acatactggt attgacttta
     4921 aaggtatgga agagcgtggt tttataacca gactcattat atttattgct attcgcatct
     4981 ttgttttgct gggtgaaata gcccagttca acacctgtct ggttatattg gtcccaaata
     5041 taggccgggc gaacaaccgc acgaatagat tcgaaatcag aatgtgcgcc tgtttcgtag
     5101 ctataaatat cgttaccgaa ggagtaaaca atcgcgttag ccacaatgaa atgatcgccg
     5161 atataagctt cgccctgcga agtcagacga actgcagttc cgcctgtgtg atcaccataa
     5221 taacgaccat taaaggtagt aaatggactt gcaccagcat aacgaccaaa gttactggcg
     5281 atagagttat tcgcaaccag gaaggagaat tcgttgaagc cacccttatc aaatttctgc
     5341 gttaaagatg taccaaacat ccaggtatct ttccagtcat aatacccgtt attatcctga
     5401 ttatcttttt ccgatgcgct ttcatttgct gagacataac gaccgctcac cattaaggtg
     5461 gctttatccc ataacgggat atctttatag cgtaaatcaa tggtattggt attaatctgc
     5521 tgtttgtttt gcaggctgcg atcgtaatca tcgacatctt cacgaaccag cgcgatatca
     5581 attttacccg gaccgacttt ccagttttcc agacctacac ctgcggcagc atcggtacgc
     5641 tgcgttttcc agtcaagcat ctggatttca attttcggcg caccgtgttt acccacccag
     5701 aaatcagcct ctggcgcaaa gggcaggaaa cctttggtgg taacgtacat atcagagaac
     5761 tgcataaagt tctcaccacc ggcgttatcg ccaaaccagc cagtagagta ctgctgacca
     5821 acgttaccat ccatcatcac gatggcatca acccgtttgc cgttttcgtt gtagacacgt
     5881 tgttttaact gcaaatcaaa ccagccggag tattcgttac caaagcggcc cagagaacca
     5941 attgcccatg atttaggtga accatgagag gctgttcccc aaccagaacg gtagtagccg
     6001 ttgtaactaa agccgatttc atctttcaca aatttactga aatctttcag agtcatggaa
     6061 gaataaatcg agcctgtctc actggcattt ttctcttcat ttttaaccag tacagcatca
     6121 ggctttgcgg cacgactggt cgggaatgga ttggcggcat acccttgatc attcgtactt
     6181 acgctacgat tcaccgtcgc tggcgtatac tttttcttct cttcatcttt atattttttc
     6241 agttcgcttt gcgtttctct taacgccttt tctaatagct caaggcgttg ttccactgtt
     6301 aatgattcag ccaatgattg tgcggaaaag gctaacggtg ccattaataa gatggcagag
     6361 gtaataagat ttcgtctaaa cataatatcc ctttatggtg caaagaaaga attaacgcat
     6421 cgcatccaga ctgttctgaa tgcgacgata attaaggtgc tttaatggtt atttttttaa
     6481 tgacagcccc cgagttttga tcacttctgc ataccatccg aagcttttct tgcgcgttct
     6541 tgtgaggctt ccttcaccat tatcatcacg atccacataa ataaagccgt agcgcttaga
     6601 catttgtgaa tgagatgcac tgactaaatc aattggcccc caactggtgt accccataat
     6661 atccacacca tcggcaatcg cttcatttac ctgtaccagg tgatcgttta aataggcaat
     6721 tcgataatcg tcctgtatcg aaccatccac ttcaacgctg tcttttgcgc ctaatccgtt
     6781 ctcgacaata aataacggtt tttgataacg atcccaaagc gtatttaaca gaatccgtaa
     6841 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat ttgggatcat
     6901 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccggt
     6961 catgtaataa ctaaaagaga tgaaatctac ggtatgtttt aaatcttctg cgtcactttc
     7021 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatactg
     7081 accacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
     7141 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccaat
     7201 tttcgcttcg gggatcaggc tatgacaggc tttaactgcc cgcgcactgg caaccagttg
     7261 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccactcccgt
     7321 gaatggcgcg tgtaatgaca tgttgatttc attaaatgtc agccataaca caactttatg
     7381 ttggtagcgg gtgaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
     7441 agcccaaccg ccgtagtttt tcaccagccc atatggcatt tcgtaatggg ataacgttac
     7501 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
     7561 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
     7621 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtgata
     7681 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
     7741 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt cctgccaggc
     7801 gccttccacc tgattggcag ctgttgcacc accccaaaga aatgtttctg gaaatgcttt
     7861 cataattaac tccttttatc gttagcgaat gatggataac agcggctcac cagcgtttat
     7921 ctgcgccgtg ccgtggggta atacgtccgt aaaatcatcg ctattactga ttaataccgg
     7981 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     8041 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     8101 tacggtatcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     8161 ggcgtgtaat gtggcgaaca acgaagcaat tcgacctgcc accggagaac gcacttcacc
     8221 aaccgagggc ataatggcaa tacctttacc caacaggccg ctggcaaacg tggtatcagc
     8281 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     8341 tgtaataacc tctggtgttt tctcttgctg ggcaacttgc gctgactgac tcttagcggt
     8401 gatgaaatga aacatcaccg taccgacaaa tgcgcaaccg atggcaatga caccgccaat
     8461 aacgctggcc catacggtga aatcaatccc cgttgacggg atggtttgca tgaaggtgaa
     8521 aatacctggc aaaccaaagg agtaaacttt cgtttgcgcg tagccaatga tggtggcccc
     8581 caatgcccca cttatgcagg cgataacgaa ggggtactta cgcggcagat tgacgccata
     8641 taccgctggt tcggtgatac caaacaaacc tgtcaacgcc gctgatcccg ccaccacttt
     8701 tttctgtgca tcgcgttcgc agaggaagac gccgagcgcc gccccgactt gcgccataat
     8761 ggcgggcatt aacagcggga tcatggtgtc gtagcccagc acggtgaagt tattgataat
     8821 taaaggtatc aaaccccaat gcagaccgaa catcacaaag atttgccaga agccgcccat
     8881 taccgcgcct gcaaatgcag gaaccgcctg ataaagccag agataaccgg cagcaatcag
     8941 ttcacttatc caggttgata gcggccccac cagcagaaat gtgatgggcg tgatgaccat
     9001 cagacatagc aagggtgtga agaaattttt gattgccgaa ggtaaccacg cattaagtcg
     9061 gcgttccaga atgctgcaca accaggcaga aaaaataatg ggaataaccg atgacgagta
     9121 attcaacaat gtgaccggaa tacccaggaa atccaggccc agcgcatcca ctttttgccc
     9181 gttctcgaag gcagtcagaa ttaatggatg cactaacgct ccgccaatca ccatggcagt
     9241 aaatggattg ccgccgaagc gtttccctgc ggtgtagccc aggattatcg ggaagaacca
     9301 aaacaaggca tcactggcgc taaataaaat taaataagta ccactttgtt cggtcgtcca
     9361 ctgaaaagtg agtgccagag ccaacatacc tttcaggatc ccggttgccg ccatcaaacc
     9421 gatcagaggc gtaaaaatac ctgaaataac ataaacaaag cggtttaaca gattaccttt
     9481 atcatcattt tccggtgcct gttgcgcttt ttcgccaagg cctgccacgc tgttaaccgc
     9541 caggaagaca tcggccacat ggttacctat gacaacctga aactggccac cgctttccac
     9601 caccataata ataccggggg tctttttcag tacctcagct tgcgctttgc tttcatcctt
     9661 taacttaaaa cgtaatcgcg ttgcacaatg catcagactc acaatgttat ctgcgccccc
     9721 gactcctgcg actattgttc tggctaactc cgtcataact tgccctctac cgctttgcgg
     9781 caaaactcca aaaaaaaacc tgaaaaaaac ggcctgacgt gaatcaagca atttttttca
     9841 ggttttgccc gcttagtgcg gtaacaatcc tttattcagt aataatattt cagtgttctt
     9901 tgcgcacgcg ctctatattt atggctaaaa acataatctc tgcaggtgag attttacgtt
     9961 gatactgcaa accaataaaa atggcgatcc gctccgcaca ttgccatgct tgcgggtaat
    10021 tttgctttac tgcttgttgt aatgattcat cactatcgtt aatcgatgca tgttcaataa
    10081 tacgccagga taaaaatttc agatgcgtaa ccagtcgctg ataactcaag ctttcttcct
    10141 ggtaattaag gctgaactga aattttatta attgcagcat ttctcgcatt aattgcgtga
    10201 cacctgcaac atcctccata tttccgctca tttgggcact gaccagatgc atggcaataa
    10261 agcccacttc atctttcggt aactgcacgc ccaaccgttt aacaataatg gttaacgctt
    10321 cttcccctag ctggaactct ttcgggtaaa gtcgctggat atcccacagc agcgggttgg
    10381 gcagtagcac gttttgctga aagcgtttaa tcgcaaactg gcaatggtca gttagcgaga
    10441 tataaatact gtcctgcaac tttcccaggc gctcctgcgc cagagagata atacgatcac
    10501 aggttgccat cacctcaaga ggcatatgac ttaagagttc gcttaatcgc ccgttcagtt
    10561 catgactgct caaggcatac tctttttcta ttccacttga gttaattctt tcgcctgggc
    10621 gtttttgaaa gccaattccg cgccccatga cgactttttc ccgctgttga tcatcaataa
    10681 ccaccacaac attattgttg agaattttgg tgatttgcat gttcatagca aggacctttt
    10741 ttataaacaa aaaaacccga cttcaccagt attctctggt tatgtcaggt tttgcctgcg
    10801 aatgcagtaa caatccagtc attaattaat ggtttttata acgaacatcc aggttcgaaa
    10861 attaatttag ttgcgtgcag agaaaatagc
//
LOCUS       DAZKGF010000167        10879 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZKGF010000167 DAZKGF010000000
VERSION     DAZKGF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.167"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            245..1969
                     /locus_tag="ACHOQV_004093"
     CDS             245..1969
                     /locus_tag="ACHOQV_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFP9632379.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKARSEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAWFKA
                     LPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRPGDR
                     CVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPP
                     FGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPC
                     YGLAENALAVSFSDEACGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEH
                     GIEIRNEADIPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDG
                     YLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRIS
                     DEERRGQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAAS
                     LHVQESLA"
     gene            1966..2880
                     /locus_tag="ACHOQV_004094"
     CDS             1966..2880
                     /locus_tag="ACHOQV_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9632380.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     IWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLIAMADEITLGIFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLNAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAEARCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SANNNRISEDINWFPGISLEHALRNGLF"
     gene            2912..3160
                     /locus_tag="ACHOQV_004095"
     CDS             2912..3160
                     /locus_tag="ACHOQV_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9632381.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            3160..4332
                     /locus_tag="ACHOQV_004096"
     CDS             3160..4332
                     /locus_tag="ACHOQV_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP9632382.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTSYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLVEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTISTQPELRQKLMDNANNLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4367..5446)
                     /gene="lptG"
                     /locus_tag="ACHOQV_004097"
     CDS             complement(4367..5446)
                     /gene="lptG"
                     /locus_tag="ACHOQV_004097"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP9632383.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVDVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETQ
                     ETSDNLPWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5443..6513)
                     /gene="lptF"
                     /locus_tag="ACHOQV_004098"
     CDS             complement(5443..6513)
                     /gene="lptF"
                     /locus_tag="ACHOQV_004098"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP9632384.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(6543..7103)
                     /locus_tag="ACHOQV_004099"
     CDS             complement(6543..7103)
                     /locus_tag="ACHOQV_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP9632385.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLRNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWHLQEQSSTILVLNKTGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(7115..7939)
                     /locus_tag="ACHOQV_004100"
     CDS             complement(7115..7939)
                     /locus_tag="ACHOQV_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632386.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPMALDSDIRQRLELLISRFPV"
     gene            complement(7939..9288)
                     /locus_tag="ACHOQV_004101"
     CDS             complement(7939..9288)
                     /locus_tag="ACHOQV_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFP9632387.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYASNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9334..10092)
                     /locus_tag="ACHOQV_004102"
     CDS             complement(9334..10092)
                     /locus_tag="ACHOQV_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632388.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(10124..10837)
                     /gene="yghS"
                     /locus_tag="ACHOQV_004103"
     CDS             complement(10124..10837)
                     /gene="yghS"
                     /locus_tag="ACHOQV_004103"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9632389.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
BASE COUNT         2733 a         2830 c         2677 g         2639 t
ORIGIN      
        1 cagagactac acagaagcgc cacaatgcct cctgtcgatt taaggcgacc ggacaaggct
       61 ccttcttaat atattcataa attaaatatc attcacaata aaaacaaaaa aactcaaaca
      121 tattatttaa atcaatgttc attttcacac cgatgaaaat actatctcat ttatctttta
      181 tgcctatata atccaccgca ttattgtcat tttattttgg atatccaatt ttttcgtggt
      241 gtatatgtct aataaaatct ttacgcattc cctacctatg cgctatgccg attttccaac
      301 gctggttgat gctttggact acgccgctct gagtagcgcc ggaatgaatt tttatgacag
      361 acgttgccaa cttgaagatc aactggaata tcagacgtta aaagcgcgtt ccgaagctgg
      421 tgcgaaaagg ttgttatcgc tgaacctgaa aaaaggcgat cgcgtggcgc tgattgccga
      481 aacgagtagc gggtttgtag aggctttttt tgcctgccag tatgccggct tagtcgccgt
      541 cccgttggcg attccaatgg gcgtcggtca gcgggattcc tggagcgcca aactgcaggg
      601 tttactggca agttgccagc ccgcagccat tatcactggt gatgagtggt tgccactggt
      661 caatgccgcg acgcataaca acaaccccga attacatgtt ttaagccacg cctggtttaa
      721 ggcattaccg gaagccgatg ttgcgctcca gcgtccagtt ccaaacgata tcgcctacct
      781 ccagtacacc tccggcagca cccgttttcc ccgtggcgtc attatcaccc atcacgaagt
      841 gatggctaat ctacgtgcta taagccacga tggcattaaa ttacgccctg gcgaccgctg
      901 cgtctcctgg ctgcctttct accatgatat gggactggtc ggctttctcc tgacccccgt
      961 cgccacgcag ctttcagtag attatttgcg cactcaggat tttgccatgc gtcctctgca
     1021 atggcttaaa ttgatcagta aaaatcgcgg caccgtttcc gttgcaccgc cgtttggcta
     1081 tgaattgtgc cagcgccgcg tgaatgaaaa agatctcgct gaactggatc tttcctgctg
     1141 gcgcgtcgct ggtattggag cagaaccgat ctccgcagaa caactccatc aattcgctga
     1201 atgttttcgt caggttaact ttgacgataa aactttcatg ccgtgctacg gactggcaga
     1261 aaatgcgctg gctgtcagct tctctgatga agcctgcggg gttgtggtta acgaagtgga
     1321 tcgcgacatc ctcgaatacc agggtaaagc cgtcgcgccg ggtgctgaaa cacgcgccgt
     1381 atcgactttc gtcaactgcg gtaaagcgtt gccggaacat ggcattgaaa tccgcaatga
     1441 agcagatata cctgtcgcgg aacgtgtggt aggccatatt tgcatctccg gccccagcct
     1501 gatgagcggg tactttggcg accaggtttc gcaagacgag attgccgcga cgggctggtt
     1561 agacaccggc gaccttggtt atctgctgga cggttatctg tatgtcaccg gacgcattaa
     1621 agatctgatt attattcgtg gccgtaatat ctggccgcag gatattgaat acattgcgga
     1681 acaggaaccg gaaattcatt ctggcgatgc gattgctttt gttaccgccc aggaaaaaat
     1741 cattttgcag atccagtgtc ggatcagcga cgaagaacgt cgcgggcagc ttatccacgc
     1801 gctggcagct cggatccaaa gcgaatttgg cgttaccgcg gatatcgatc tgttgccgcc
     1861 ccacagtatt ccccgaacat cctccggcaa gcctgcccgt gcggaagcga aaaaacgtta
     1921 tcagaaggct tatgctgcca gtcttcatgt gcaggaatcc ctggcatgaa tcaaactgtc
     1981 gcggtgacgg gcgctaccgg gtttatcggt aaatatatta ttgataacct gctcgcccgc
     2041 ggctttcacg ttcgcgcatt aacgcgtact gcccgcgctc acgtcaatga taatctcatc
     2101 tgggtgcgcg gttcgctgga agatacacat tcgcttagcg aactggttgc cggagccagc
     2161 gcggtggtcc attgcgccgg acaagtgcgc ggacacaaag aagagatttt tacccgctgt
     2221 aacgttgacg gcagcctgcg cctgatgcaa gcggcaaaag agagcggctt ttgccaacgt
     2281 tttctgttta tctcttcgct ggcggcgcgc catcccgaac tctcctggta cgcaaattcc
     2341 aaacacgtcg ccgaacaacg actgatcgca atggctgacg aaattacgct gggcattttt
     2401 cgcccgacag ccgtgtatgg tcccggcgat aaagagttaa aaccgctgtt tgactggatg
     2461 ctgcgcggcc tgctgccacg acttggtgcg ccagatacac aactctcttt cctgcacgtc
     2521 accgattttg cgcaagcagt gggtcagtgg ttaaacgccg aaactgtaca gacgcaaacc
     2581 tatgaattat gcgatggcgt cgctggcggc tatgactggc aacgcgtacg acagcttgcc
     2641 gccgaagccc gttgtggttc tgtgcggatg gttggcattc ctctgccgct actcacctgc
     2701 cttgcggata tcagtactgc gttgagtcgc ctggcaggta aagaacctat gctgacccgc
     2761 tcgaaaattc gtgaattaac ccacgccgac tggtcggcaa ataataaccg tatttctgaa
     2821 gatattaatt ggtttcccgg gattagcctg gaacacgcat tacgcaacgg gctattttga
     2881 ttgtcttgtt taaaggtgtg ttaacgtttc tatggtaaat cgtgaaatag taatggatta
     2941 catcctttcc tgtttacagg atttggtcga aaacggtgtg gaaattaagc ccgacagcga
     3001 tctcgtcaac gatcttggcc tggagtcgat caaagtgatg gatctcttaa tgatgctgga
     3061 agacagattc gatatttcca ttcctattaa tattctgctg gatgtcaaaa cccctgcgca
     3121 attaatggaa actctacttc catggctgga gaataaataa tggggctata cgataaatat
     3181 gcgcgccttg ctggcgagcg cctgcaattt agtgacaacg ggttaacacc atttggcacc
     3241 tgtattgatg aagtgtattc cgccaccgaa ggccggatcg gcaataaaaa agtgattctg
     3301 gccgggacca ataattatct cggtttaacc tttaatcatg atgctatccg cgaaggtcag
     3361 gcagcgttag cggcccaagg cactggcacc accggttctc gtatggcaaa cggcagttat
     3421 gcgccgcatc tggcgttaga gaaagagatt gccgagtttt tcaatcgccc taccgctatc
     3481 gttttttcaa ccagttatac cgctaattta ggtgtcatca gcgcacttgc cgaccataac
     3541 gccgtggtgt tactggatgc cgacagccac gccagtattt atgacgcctg ttctctcggc
     3601 ggcgcagaga ttattcgttt ccgccataat gacgtcaacg atctggaacg gcgcatggtc
     3661 cgcctcggtg aacgggcgaa agaggcgatt attatcgttg aaggcatcta cagtatgctc
     3721 ggtgacgttg cgccgctggc agaaattgtt gatatcaaac gtcgtctcgg tggttatttg
     3781 attgtcgatg aagcacattc gtttggcgtg ttaggcgcaa caggccgcgg actggttgaa
     3841 gccgttggcg ttgaagacga cgtggatatt atcgtcggca ccttcagtaa aagtttggct
     3901 tccattggtg gttttgccgt gggttcagaa gcgatggaag tgctgcgcta cggcagccgc
     3961 ccgtatattt ttaccgcctc tccttctccg tcctgcattg cgaccgtgcg ctcgtcgtta
     4021 agaaccattt ccacacaacc agagctgcgg caaaaactga tggataacgc caacaatctg
     4081 tatgacggtc tgcaaaaact gggttatgag ttaagttcac atattagccc ggtagtgcca
     4141 gtaataatcg gttcgaaaga ggaaggttta cgcatctggc gcgagcttat ttcgctgggc
     4201 gtttatgtga atcttatcct gccacctgcc gcgcctgcag gcatcacgct gctgcgctgt
     4261 agcgtaaacg ctgcccacag tcgtgagcaa atcgatgcca tcattcaggc atttgctact
     4321 ctgaagcagt aatgtgagcc cgctgtggtg ttgcgccacg gcgggtttaa tgttgtctgt
     4381 cataccaata caccaggctc aacgccacca gcacaaacgc taccggtaaa ccgagcgcgg
     4441 taacaggtgc gctcaacgca aataacaatc ccagattgac catgatttga tagctgatcc
     4501 aggtgagtaa gccaacgatg acgcctacag cgagacggct acccatccca ggcgaacgcg
     4561 gggcgctaaa ggtaaacggc acagccagca aaatcatcgc cagggtcaag atgggttgtc
     4621 ccagtttttc ccacagtgca aggcggtatt cgctgctggg ttgcccggtt tcctgcaaat
     4681 aatggatgta atgattaagt tgacggacag aaaaagtatt tcccggcatc gataactctt
     4741 caagattcat actggtgaag gccgattgcc agggaagatt atcagatgtt tcctgcgttt
     4801 ctttgccatt cagccatttt ttatgattca caccatgcag gatccacgtt ttgtcgtctt
     4861 caatggtagc actgcgcgca taaatgtagg attcgagcga gagatcatcg cggtaatgaa
     4921 atatctccac gtccaccggc tggccctgct cgttcagcga tttcaccgtc acaaattcat
     4981 taccacgcct ggcccacagc atattgccgg taatgtcatc gtcctccccc aacgcagtgg
     5041 cagtcgattt gatttgcagc gcctgttgct gcaatggcga cgccacccac tcatctatcg
     5101 cgcctaatga aacagtccac aatatcccgg caaccagcgc caccagtgca atacggaaga
     5161 tagaaaaccc cgtgctgcga atggcggtaa gctcactgtt tttcgataac tgccccaggc
     5221 cgacaatccc tcccaataag gcgataaacg gcgaaagttc gaccagtgtg cgcggtaagg
     5281 tcatcagcac caccagcacc gcctgagtcc agcgataacc acccgggctg acatcatcca
     5341 gttcgttaat caggttgaag gtggtaaaaa gcggcagcaa tagccctgcg gcggcggcaa
     5401 aaccgagaaa gagatgacgg attaaatagc gactgaaaac attcatcggg aaaatttctg
     5461 cagcaaagag aagtcgcgtg cgaccaacat cagcaggccc attagcatga gtccgggtac
     5521 taaccacaaa ccaggaatga ggggaatcgc gccgttagcc accagcgtac ggcagacgtc
     5581 gccgccataa aaaatggcaa caaacagcaa cgtcagtggc aataacgttg aaaatcgccc
     5641 ttgccgcggt tttacccgac ttaatgaaat ggccagcagc gccatcaaca atgctgtcag
     5701 gccacggctt tgtcgccatt gcagttcggc atgatcggca ggaaaggcgg agcgcgccag
     5761 ctccgtgacc gattttgctt tgcgtttgac gttagggctt tgatccagcg gattcagatg
     5821 taattgcaga ttacggtaga tctgttcgtt gtcgtcacgc ccctgatgat cgagaagata
     5881 ggcggtcccg ttatgcaaca taacggtcgg tttctcaggt gatgggtcaa ccacatcaac
     5941 cgaacgggcg cggaaaatgc gggttcgatt agcagtagaa gtataaatca gcgcatcagt
     6001 caggcgatta ttatcctgat caaccgtctg cgaaaggatc attcgtccgt tatcgttagt
     6061 gttaaatttc tttgcccgca actggcgaac atccagctcc gactgtgact gttgctccag
     6121 ttgataaatc tgcgcataag cccacggacg accatacatc gacagaaggg tgacaaaaat
     6181 acttagcggt atcgccagat aaagaacggc tttgtacaag cgtcccggac tgccccccgc
     6241 cgcagagata gcggtaattt ccgagtcggt atacatctgc cctagcgtca cgccaactga
     6301 cacatacagt ccaacaggta acaacatctc cagtgcaatc agcactttgt agaaaacgat
     6361 atccagcaca acatcaagag ctaatgtccc atttgccgct tcggtcagat aacgctgtgc
     6421 ggagtagctg gcgaaaataa agatcagaaa accgacgata atcagcacca gacggcgcgt
     6481 accacgcatg atgtagtgtt caaccagctt catctcacta acgccctcaa tcctttgaat
     6541 ttttattcat tgatcagctt ctgcgccagc gcaatgacat gatctacctc ttccggcgtc
     6601 aggctgccgt cctttgccca ctgaatttgc ccggttttat tcagcaccag aatggttgaa
     6661 ctctgctcct gcaagtgcca ggcaacgcgt ccctgtccat tgccgtcaat aacgaattgc
     6721 gcccatggat agcggcgttt atttttttcg attttgccaa cgacaaaata gccggtacca
     6781 aagatggcat catcagtgtt cacaatggtg gtgggctgaa aacgatcctg cggaaagttt
     6841 gccgcttcaa ctgctttaat taacagtgag ttctttttct tcgccgactt acgcccggcg
     6901 atatactgaa taatgcgaac ttttcccgcc aattgggtgc tgttccactt ttggtagtga
     6961 atttcgtcgt cgctattccg aagcagctcg ccgccttcct ggatataaac gggggtaacc
     7021 gttttgccag tgacgaaatt gtgtgcctgc acacccgggg ctaagagcat gataatcagc
     7081 gcaataatca aacgagatga catcattgct ccggttatac cggaaaacgc gaaatcagca
     7141 gttctaagcg ctggcgaata tcgctatcca gcgccatcgg tgcatcatac gactggcggt
     7201 tttccatatc atccgccacc atgtcgtcgt gtttatcccc catcaggcca ttaatggcgg
     7261 cgaggaagcg ggaaatctgt aacgcttctt tattttcagg atcaagcgat cttagggtgt
     7321 cgtaataaga aacctgctgg ctgatgattt ttcgcacatc atgaaattcc ctgccggtta
     7381 gtcgcggttt ggcaattatc tctttcaggg tatgcatttg cttttgccga taggcaataa
     7441 aaccgcttac gctttctaac tggctatggc gtaatgaata gttcaccaac gaccagaccg
     7501 gagagcttaa ataaacccgt aacaggttgc cgtaatacga cacaatattt ttcttaccgt
     7561 tacgaaagcc gtcctgaaaa cgccccaaaa aaacggtcgt tttgccgaat aaaaatccgg
     7621 cctgatgctt ctttaaatac agccgttgcg caaaacgcag gtgcttgtag cgcgctcgca
     7681 tgtatttaaa ttgtttgcgc tcatcagcag tcagctcatc gcctttggcc tgtttgcata
     7741 ttaaacgcag taactcctgg cgattgacgc cattgagcca gaattgcagg cataacgcgt
     7801 aattatcatg gatgatttcg gtggtacaac ttagtgcgat agtcggcggc aaactgacct
     7861 gtgcatcaac gacatcatct tcttcaatcg caacaaatag acgctcgctc gcctctagcg
     7921 ggaaatattt tctcatcatc aggatttctc tccggctgcg ttataaagca aggaatggcg
     7981 ctgaaaaaat gcctttaagg tcggaattag cgaaagcacc acatccagac attgaccacc
     8041 gaaataagca aacgctgctc ccagcacgcc aaacagatga cagaacatga acagcaatac
     8101 gatgtagccg attgaggcga tggtttgcgc cacgagaaaa gcgcgctgtt ttcccgccat
     8161 caatagcaat gactcctgag ggaagcccag catcgagatc acaattgcgc ccagcatcac
     8221 ctgaatcagg tcatacgctt cgagatactt aacgccaaac accagcgaaa tgagcggctt
     8281 gccgacaatc aacaccgcga gcgccaccag aataccgatt ccgcccgcca gtaaacctga
     8341 cttcacgcct aacaaccacg gtctggtggt acgcggatct aaacgcatca cctctgggta
     8401 aaaacttttg cccagtaaac ccgctggcgt tccggcggcg tcgaagaatg tcatggcgat
     8461 tttaaataac ccggcggcag cgggtcctaa cacgatcccc accaacactg tgctgcacga
     8521 attacgcgcc gaccagatgg aatgggcaat gttggttgac cagacaaaac tccacgcgcc
     8581 tttgatgtgt cgggcagact caaacagatt caatttgaac gcgttatgga tatttctgcg
     8641 gcgtaattca cgtgcggcaa accaccagta catggtgccg ccaacaagat tcgaggcgta
     8701 ccaggcaata acaaaacccg caaaaccaaa gtcaaaatac caggcaacga cgctccccgc
     8761 tgcgcgcaga aaaggtttcg tcgcttgctg tacagcaatt agatcgaagc gatctaccgc
     8821 acgcagaatg ccggtcggcg tggaggaagc cattgaagga atgagcgtgc aatacagcgc
     8881 tgccagccaa aagctttggt catccagacc taatgaatgg gaaagaaaag ggagtaaggc
     8941 aatgccgccg acaatcgcca ccgcgccgct gacgatatcc agcgagaagg aaaatgagac
     9001 gacattgcgg aattgctgcg gattattgtt ggttaatgct ggtgttccgt actgaaccac
     9061 cagctgccat gtctgaaact taataaaatc gctgatcgac ttggcgtacg attgtacaat
     9121 caccagtacg ccaaacatgg cgggcgtcat ccctttaccg gcacacgaga gcgccaacag
     9181 acccagcaaa gcgctcacga cattactgga gcctaaccag gcgctattgc gaataatggt
     9241 gcgaaacgcg ccatctgcaa accaatgttt gatgttaaaa cccgccaatt cagcctgacc
     9301 ttatcgttga tagtaaaaag tatcccgcca gccttaagtt aaacttcggc ggtcagaaac
     9361 gatggcaacc agagaaaccg ccttctgtgc ctgttccagc acttcgctgt agggcgctct
     9421 ggaatcaatc tcaagaattt ttgtgccgtt atagccaatc gtcgacatga cgccgatttt
     9481 gtcctgcagc tcggcatagt catggtcagg cttgcgggag atggcagtat caatatcaat
     9541 ccccaaacga atgattaatt caggacggta ttgcgccatt tgttggtata aacgccgttc
     9601 gcgctgcgcc agaaacatgc tgattttccc ggtcgcacgt tcgacgccga tccccggtcc
     9661 gtcataataa aagcccgaaa tttcagcctg cgggaagcga tcgctgacca ccagaacgcc
     9721 actttgcgcc agtcgctgaa ccttgcgtag attcgccatt cttcgcagcg agaagcagta
     9781 cataatcacc gccgcccata gcgctggaga tttggttttc atgctttggg ttttcgagga
     9841 tttggccgcc agtcgccgtt cgagccagac gccaaccaac ggcaatcgtt tgattttgtc
     9901 gccgtcttcg cccgagagca gccccagata gcgccgctcg gtttgccagt gttgttgcag
     9961 cgatttcacc aggtcggtgg tcagtgtgga tttaccggtg ccatcacacc cgaccaccgc
    10021 aatcagcccc ggaatgtagt tgggctgcgg cgcggttgta ctattaacag tttgtgtttg
    10081 tagtgcgtcc attcgttaaa tcatccccta tccattttgg tgatcaggaa agcgcggcgt
    10141 gaattgcgcg tagtgacgct tgcagaattt catcggcggg ttgccgacca tccagttcaa
    10201 ggatctttgc gccattaaat gtcagttgcg gcgtaacggc gattttttcc tgtaacgccg
    10261 ccagttggtg gtcaggttta cgcgcaaacg cggtttgttc atcaatgcca agacgaatca
    10321 acaatacggg caaataagac gccatccatt ggtacagctt cagctcgcgc tgccttaaca
    10381 tttttatcca accgttaccg cccgtggttt ttgccaattg cgggccatca aagcgaaaac
    10441 ccggcacttc aacctgcggg tagcggtcgg tgatgagcag atagccttgc tggcttttac
    10501 acaacatttt gcgaaacttg tacgcccgcc agcaggaaag cagatagata actagcgcag
    10561 taatattgcc aggtggtgtg gagggctttt cgtgcacatg tgccgcttta cttcgcagat
    10621 aacgcccaaa aggtgcgcca ataacaggga gctgtgaaat ccattcgcca attcgcccgg
    10681 acgattgccc gagataaatg tgttctgttg gcattgttgc tgccagttca tttaccaggc
    10741 ttgccgtgag ggtcgattta cctgaaccat cacatcctac aatggcaata acacgcactg
    10801 gcgtggaatt aattattgac atattacgtt gattcacgaa aaacccggca gcaaataatg
    10861 tattgaatat tacgtggtc
//
LOCUS       DAZKGF010000168        10776 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZKGF010000168 DAZKGF010000000
VERSION     DAZKGF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.168"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(159..1211)
                     /gene="fbaB"
                     /locus_tag="ACHOQV_004104"
     CDS             complement(159..1211)
                     /gene="fbaB"
                     /locus_tag="ACHOQV_004104"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFP9632390.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1468..2745
                     /locus_tag="ACHOQV_004105"
     CDS             1468..2745
                     /locus_tag="ACHOQV_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFP9632391.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2742..3746
                     /locus_tag="ACHOQV_004106"
     CDS             2742..3746
                     /locus_tag="ACHOQV_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP9632392.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAYFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            3743..4708
                     /locus_tag="ACHOQV_004107"
     CDS             3743..4708
                     /locus_tag="ACHOQV_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP9632393.1"
                     /translation="MSGARLHTLLPELATRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAATLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4682..5428)
                     /locus_tag="ACHOQV_004108"
     CDS             complement(4682..5428)
                     /locus_tag="ACHOQV_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9632394.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5480..6298)
                     /locus_tag="ACHOQV_004109"
     CDS             complement(5480..6298)
                     /locus_tag="ACHOQV_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFP9632395.1"
                     /translation="MQLRITSRKKFTALLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(6363..7163)
                     /gene="thiD"
                     /locus_tag="ACHOQV_004110"
     CDS             complement(6363..7163)
                     /gene="thiD"
                     /locus_tag="ACHOQV_004110"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP9632396.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWTDTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7160..7948)
                     /gene="thiM"
                     /locus_tag="ACHOQV_004111"
     CDS             complement(7160..7948)
                     /gene="thiM"
                     /locus_tag="ACHOQV_004111"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFP9632397.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAI
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVASSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(7983..8079)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8171..8443)
                     /gene="rcnR"
                     /locus_tag="ACHOQV_004112"
     CDS             complement(8171..8443)
                     /gene="rcnR"
                     /locus_tag="ACHOQV_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFP9632398.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            8565..9389
                     /gene="rcnA"
                     /locus_tag="ACHOQV_004113"
     CDS             8565..9389
                     /gene="rcnA"
                     /locus_tag="ACHOQV_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFP9632399.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGKRNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            9608..9946
                     /gene="rcnB"
                     /locus_tag="ACHOQV_004114"
     CDS             9608..9946
                     /gene="rcnB"
                     /locus_tag="ACHOQV_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFP9632400.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPDMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10028..>10776)
                     /locus_tag="ACHOQV_004115"
     CDS             complement(10028..>10776)
                     /locus_tag="ACHOQV_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFP9632401.1"
                     /translation="SNNQTSFTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSS
                     ASSSTRGSAAVQFLLCLLGGKSWDACVNSYRNALAQNAGVYSFNLALSYNPITTTCKP
                     DDLLINLESIPVSQLPATGNKATINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDL
                     LAGSNTILKGAEDNGVGFMLESNSSPVTFLNITDNSKGYTNLKEIAAKSKITDTTVSI
                     PVTASYYVYDKNKVKSGALQATALINVKYD"
BASE COUNT         2523 a         2754 c         2703 g         2796 t
ORIGIN      
        1 cgatcacaaa aacgacaata tgaaaatcaa tccaggaatg aaaggcaaaa aaaacggcct
       61 cccgggggaa gccgtaacaa agtacgcgtg gttttatgcc ggatgtggcg ttaacgcctt
      121 ttccggccta cggggtagtg ctgttcgtat gaagcgcatc aggcgatagt aattttgcta
      181 tcgagataaa cgtcctgcac ggcgttaatc agtttcacgc cgtcagccat cgatttcttg
      241 aacgccttac gtccaagaat cagccccatt ccgcctgcgc gtttgttgat aaccgcagta
      301 cgcactgcat cgctgaggtc agtttcaccg cctgcagcac cgccggagtt tatcaacccg
      361 gcccggccca tatagcagtt agctaactga taacgcacca ggtcaatcgg gttttcactg
      421 gtcagtttgc tgtagactcg atcgtcggta taaccgtaat taatcgcttt atagccgccg
      481 ttattttccg ccattttttg tttgacgata tctgcaccta tggttgccgc cagatggttc
      541 gcctgaccag tcaggtcggc ggaaacatgg taatcaacgc catctttctt aaaggcggag
      601 ttacgcaaat aggcccacag cactgtcacc atgcccagct cgtgcgcacg ttcaaaagcc
      661 gcagaaattt cttcaatctg gcgacgtgac tcttccgagc caaaatagat agtcgcacca
      721 accgccaccg cgcccatatt gaacgcctgc tccacgctgg catacagcgt ttgatcgtag
      781 gtattcgggt aacttagcgt ctcgttgtga ttaagtttga cgaggaatgg aatgcgatgc
      841 gcgtaacgcc gcgataccga tgccagcacg ccgtaagttg acgccacaca gttacagccc
      901 gcttcgatcg ccagttcaac aatgtttttc gggtcaaagt agagcgggtt agcggcaaat
      961 gaagctccgg cagagtgctc aacgccctgg tcaaccggca gaatagaaag atatcctgtg
     1021 ccagccagac gcccggtgtt gtacaacgtc tgcatattac gtaacaccgc tggcgggcga
     1081 ttattgtcaa tcatcacgcg gtctacgtag tcatgtccgg ggagataaag ctggtcagaa
     1141 ggaatagtca tacaacggtg ctgtaaaagg ttgtcggcgt ctttgccaag caactgcgca
     1201 atatctgtca ttactatgct cccgtaaatt ccgattggat atcggctatg gattttcctg
     1261 gcccgccttt tgcgggcaat cataatcctg gtcgttacag gcgagttttt ccatcttttg
     1321 gatgtttttt cagcgttttc tgttggcacg attcatcaga aaaaatgtta tcgcggtcaa
     1381 gttttcaccg caaaggtatt taaaaggtat tattaaatgg tattgtcatc gcgtacctta
     1441 cattacctgt catgaaggaa ttaaaagatg aaaacaacag caaagctgtc gttcatgatg
     1501 tttgttgaat ggtttatctg gggcgcgtgg tttgtgccat tgtggttgtg gttaagtaaa
     1561 agcggtttta gtgccggaga aattggctgg tcgtatgcct gtaccgccat tgcggcgatc
     1621 ctgtcgccaa ttctggttgg ctccatcact gatcgctttt tctcggcgca gaaagtgctg
     1681 gcggtattga tgttcgctgg tgcggcgctg atgtatttcg ctgcgcaaca gaccactttt
     1741 gccgggttct tcccgttact gctggcctac tcgctaacct atatgccgac cattgcgctg
     1801 actaacagta tcgcttttgc caacgtgccg gatgtggagc gtgatttccc acgcattcgt
     1861 gtgatgggca ctatcggctg gattgcctcc ggtctggcat gtggattttt gccgcaaatg
     1921 ctgggttatg ccgatatctc tccgactaac atcccgctgc tgattaccgc cggaagttct
     1981 gctctgctcg gtgtgtttgc gtttttcctg cccgacacgc cgccaaaaag caccggcaaa
     2041 atggacatta aagtcatgct cggcctggat gcgctgatcc tgctgcgcga taaaaacttc
     2101 ctcgtctttt tcttctgttc attcctgttt gcgatgccac tggcgttcta ttacatcttt
     2161 gccaacggtt atctgacaga agtcggcatg aaaaacgcca ccggctggat gacgctcggc
     2221 cagttctctg aaatcttctt tatgctggca ttgccgtttt tcactaaacg ctttggtatc
     2281 aaaaaggtat tgttgcttgg tctggtcacc gctgcgatcc gctatggctt ctttatttac
     2341 ggtagtgcgg atgaatattt cacctacgcg ttactgttcc tcggcatttt gctgcacggc
     2401 gtcagttacg atttttacta cgttaccgct tacatctatg tcgataaaaa agcccccgtg
     2461 catatgcgta ccgccgcgca ggggctgatc acgctctgct gccagggctt cggcagtttg
     2521 ctcggctatc gtcttggcgg tgtgatgatg gaaaagatgt tcgcttatca ggaaccggta
     2581 aacggactga ctttcaactg gtccgggatg tggactttcg gcgcggtgat gattgccatt
     2641 atcgccgtgc tgttcatgat ttttttccgc gaatccgaca acgaaattac ggctatcaag
     2701 gtcgatgatc gcgatattgc gttgacacaa ggggaagtta aatgaaaaca gaacgtattc
     2761 tcggtgctct ttatgggcag gcgttagggg atgcgatggg gatgccctcc gagctttggc
     2821 cgcgcagtcg cgtcaaagcg tactttggct ggattgaccg ttttctgcct gggccaaagg
     2881 agaataacgc ggcctgttat tttaaccgcg ccgaattcac cgacgatacc tcgatggcgc
     2941 tgtgtctggc ggatgcgtta ctggaacgtg aaggcaagat cgatccggat ctgattgggc
     3001 gtaatattct cgactgggcg ctgcgtttcg acgcctttaa caaaaacgta ctaggtccga
     3061 cctcgaagat tgctctcaac gccattcgcg acggtaaacc cgttgctgaa ctggaaaaca
     3121 acggcgtgac caacggcgca gcgatgcgcg tctcgccatt aggttgtttg cttccggcgc
     3181 gtgatgttga ttcctttatt gatgatgtgg cgctggcctc cagcccgaca cataaatccg
     3241 atctggcggt tgcgggggcg gtagtcatcg catgggcgat ttctcgtgcc attgacggag
     3301 aaagctggtc agcgattgtt gattcactgc cttcaattgc gcgacatgct caacaaaaac
     3361 gcatcactac cttcagcgcc tcactggcag cacgtctgga gattgcgctg aaaattgtgc
     3421 gcaatgccga cggcaccgaa tccgccagcg aacagcttta ccaggtcgtt ggcgcaggta
     3481 ccagcactat tgagtccgtt ccgtgcgcca ttgcgctggt tgaactggca caaaccgacc
     3541 cgaaccgttg cgccgtcctg tgcgctaacc ttggcggcga cacagacacc atcggtgcta
     3601 tggcgacggc aatttgtggc gcgttgcatg gcgttaacgc tatcgatcct gcgttaaagg
     3661 cggaactgga tgcagtaaat cagcttgatt tcaaccgcta tgccacggcg ctggcgaagt
     3721 atcgccaaca acgggaggcg atatgagcgg cgctcgatta cacacgctgc tgcctgaatt
     3781 agccacgcgt cagccagtga tggtcgtcgg cgcggcggtc attgatgtga tcgccgacgc
     3841 ttatgccctc ccctggcgtg ggtgcgatat cgaactgaaa cagcagagcg ttaacgttgg
     3901 cggctgcgca ctgaatattg ccgtggcgtt aaagcgcctc ggcatcgaag cgggtaatgc
     3961 cttgccgctc ggtcagggcg tgtgggcgga gattattcgc aaccggatgg caaaagaggg
     4021 cttaatcagc ctgatcgata acgccgaagg tgataacggc tggtgtctgg cgctggttga
     4081 gccggatggc gaacgcactt ttatgtcatt cagcggtgtg gaaaatcagt ggaatcgcca
     4141 gtggctggcg cgattaaccg ttgcgcctgg cagcctgctc tatttttccg gttatcaact
     4201 ggcctcgccc tgcggcgaat tgttagtgga atggctggaa gagctgcaag acgtgacgcc
     4261 atttatcgat tttggcccac gtattggcga tatcccggat gcattactgg cgcggatcat
     4321 ggcctgtcga cctttagtgt cactcaatcg tcaggaggct gagattgccg ccgaacgttt
     4381 tgctttatcc gcagagataa caacacttgg caagcaatgg caggagaaat ttgctgcgac
     4441 gttgatcgtt cgtctcgata aagagggcgc atggtatttc agcaacgacg cttctggctg
     4501 cattccggca tttccaacgc aagttgtaga caccattggg gcgggcgaca gtcatgccgg
     4561 cggcgtactt gccgggctgg cctctggtct gccactggcg gatgccgtat tactgggcaa
     4621 tgcagtggcg tcgtgggttg tcgggcatcg gggcggtgat tgtgcgccaa cgcgcgagga
     4681 actactcctc gcacacaaaa acgtatagat cgctgcgaca gtggctgatg ctgtactcaa
     4741 taggccgttg ctgttggtca agcgccactt gcttgatcac cagcaccggt attttgctat
     4801 ctaactgaat atgtgactga aactcggcat ccggcatccg ggcgctaacg cgggaacgcg
     4861 tacgttgtgg gtaaatgtgc tggctgcgga agtagtcata aagagaaatc ccgatggcat
     4921 caacatcatg aattaaatgt gccggaaccc acgattcctc aatcgatacc gcctcttcat
     4981 cgacatagcg aatacgctta agcaaaaaga catcacttcc tgcttcgacc gctagctgct
     5041 gcgcgacttc ttcgggacat ttcacgacac gcttgttaac ccatagcgta tcgggctttt
     5101 tcccgcgcaa taccacctgc tgagaaaaac ctcgcgcttc tttcaacgag tattcgaaga
     5161 tgttgttgat ctgcgtgccg taaccacgcg aacgcgtcac cacgccctct tcttccagcg
     5221 cctgcatcgc cttgcgcacc gtaatgcgcg aaacgccggt taactgactt aaatcacgct
     5281 caccgggcaa aatattgcca tgctccagca ccccgctgcg cacggcattt tttaccgttt
     5341 cggcaaactt aatataaagc ggcgtgttat ccgccgctaa aatacgttcg ttcagttggg
     5401 caatcagctg ggtatgcgct tgttccattt gtctttttcc tgccagggag tctggtggga
     5461 gtatatcact gcttcttatt taaggcgttt ctttaatccc atcaacgaat ccctgcaact
     5521 cctccaccgt gccattaaac acattaaaat ccaccggacc attaatgcca tctacctgtc
     5581 cacggtcgga atgctgccag aagcgccagg ccattccgtc attgtccgga cgacgttgat
     5641 agtagtgagc cacccaccac ggatattcat taaaatagcc cgccagattg gtgtgataaa
     5701 aaacggctcc tgagtaaata attggctttt ttcccgtacg tttttcgacc atttttagcc
     5761 actgacttac ccgcttgcgt aattcttttg ccgataattt tcctcgctct tctacgtcca
     5821 gtaccgcagg gaaatcgcct tgtgagaaat ccaccgtttg cagaaataat cgcgcctgaa
     5881 ctgaagcgga tactgacggg gagaaataat gatacgcccc gcgcagcagg ccattttcgc
     5941 ggcttagttg ccagttacgc gaaaaatagg ggtccaccag cttttcgcct tccgtcgcct
     6001 taataaaagc aaactgtaag cggataccgt tgtcgcgcat ttttgccacc cgctgccagt
     6061 cgatccgctc ctgccagcgc gaaacatcta ttccgtgaat ggtgtaactg gcaggaatgc
     6121 gaatagcgaa agatttaacc gggcgataac cgtagaagtg gatgtagtct ttaatctgaa
     6181 ttgctgtcga atagaaaaaa ttcaccgtct gacgcggata aattgcaacg atggaaatta
     6241 gcccaagagc gcataataaa gcggtgaatt tttttcgact ggtgattctt agctgcattg
     6301 gtttcatccc tgaatgtcag cgccaaaggc tgacaataac caaagcagct atagtacggt
     6361 gctcaccacc aggcgtggaa gtggtgaacc ggaccaatac cgtgaccaac ttccagcgtg
     6421 tcggcctggg ctaatgccga tgaaagccag ctttttgcct cctgtacggt gtcagtccag
     6481 tttgtatggc gcgggcgcag tgcagccaac gccgcagaga gcgtacaacc agtgccgtga
     6541 gtgtttttgg tcataatgcg ctgtgcggta aaccgttgtt caccctcgcg ggtaaacagc
     6601 cagtccgggc tttgctcatc atccagatga ccacctttca ttagcactgc gccacagccc
     6661 atcgccaaca gcgatcgccc ttgttccagc atttcctgtt cggtgcgcgc gtgtggcgcg
     6721 tcgagcaagg cggctgcttc gggcaagttt ggcgttatta atgaaacctg tggcaataat
     6781 cgactgcgca gcgtagcaac cgccgaaggt gaaagcagcg ggtcgccgct ttttgccagc
     6841 ataacggtgt cgagtaccac gttttggatc tgataacgtt gcaaccgttc tgccaccgct
     6901 tcaacaatat cggtttccgc caacataccg attttagtgg tgtcgattcg cacatcgctg
     6961 aacaccgaat cgagctgggc ggcgacaaaa tcaggctcaa tgcgatacac cgactgtaca
     7021 ccacgggtat tttgcgccac cagtgcagta ataactgagc aaccataagc gccaagtgcc
     7081 gagaaggttt taagatcggc ctgaatcccc gcaccaccac tcggatcagt accggcaatc
     7141 gtcagagcgt taattcgttt cacgcttgca cctcctgcgt caattgccag agcgcatcaa
     7201 ggaaatgtgg aacaaaactg cctggcccct cgctactggc gactgcgcgt tctccggctt
     7261 gtttcatcca gtgacaggca gatgcgatat tttccagcgt atcgcctggt agcgcacaac
     7321 aggcagcgac aaccgccgat aatgcacagc cagttcctac cactttggtc attaacggat
     7381 cgccaccgtg aatgccaatg atacgatgtc catcggtaac ataatccacc tcgccagtga
     7441 ccacgacgat tgcgccagtt tcccgtgcca gtgtttgtgc agcgggtatc gcgttagctg
     7501 cggcatcagt ggtatccact ccccgtccac cattagcaac gccagctaat gccatgattt
     7561 ccgaagcatt accacgtatg gcggttggct tatgggacag aagttccaca caaaaacggc
     7621 ggcgataatc gagcgcacca accgcaaccg gatcaagcgt ccagggcgtt tgtgatcgtg
     7681 ttgcttgctc aacggcagca ctcatcgctt gcgctcgtgg ctgcgtcagc gtgccaacgt
     7741 taatcaacaa ggcactggcg atagccgcaa actgactggc ctcttcggtt tcgataacca
     7801 tcgctggcga tgcaccgatc gccagcaagg tattggcagt aaaagtttgc accacatcgt
     7861 tggtcatgca gtgcacaaga ggggaatgtt ggtgaaaaag gtgtaacgtg tgcgcagatt
     7921 gcgcggaact cagcaggtcg acttgcatag tttgctcctg ccataacgtg aagaagcaat
     7981 gacctggtgg tccgtgactt ccctacgctg gcattatcca gatcaggtga tacgggtatt
     8041 tctcagcctt cacaaagaag ggcaccccga gtcgtttggt tgcgataata aatcgcagag
     8101 gaggatggta atgtccagcg cacgcgttgt aaacggggaa aaacggcggt attacaatcg
     8161 cgttaataaa ttatttgata taagaatcca gcaccttcag aacgacatcc agatcttctt
     8221 cacgttttag ctcatccccc tggtgaacga tgtgttccgt cagatggcct ttaatcactt
     8281 cccgcatcag accgtttacc gcgccacgga tagcagcaat ctgttgtaaa actgcagcgc
     8341 attcatgcgg ttcgtcgagc attttcttga gcgccacgac ctggccctga atcttactgg
     8401 cacgcgcttt cagtttctgt ttatcacgga ttgtatgaga catggcaaca cctggttaac
     8461 aagaatatga aaaatcatag cactattaat ctactggggg gtagtataag gtactggggg
     8521 gggagtagaa tcagattgcc gaattaatac taagaattat tatcatgacc gaatttacaa
     8581 cacttcttca gcaaggaaac gcctggttct tcatccccag cgccatctta cttggtgcgc
     8641 ttcatggcct ggaaccgggg cactcaaaaa cgatgatggc ggcgtttatc atcgccatca
     8701 aaggcaccat taaacaggcg gtgatgctcg gactggcagc aactatttcg cataccgcag
     8761 tagtctggtt aattgccttt ggcgggatgg tgatcagcaa acgctttact gctcaatcag
     8821 cagaaccgtg gctccagttg atttccgcag tgatcattat tagcaccgcg ttctggatgt
     8881 tctggcgtac ctggcgcggc aaacgcaact ggctggagaa tatgcacgag catgatcatg
     8941 agcatcatca ccacgatcac gaagatcacc acgaccatgg acatcatcac catcacgaac
     9001 atggcgagta tcaggatgcc catgcacgag cccatgccaa tgacattaaa cgacgctttg
     9061 atggtagaga ggtcaccaac tggcaaattt tgttatttgg cttaaccggt ggccttatcc
     9121 cctgcccggc agcaattacc gtgctgttga tttgcattca gttgaaagcc ctgacactgg
     9181 gcgcaacact ggtcgtcagt ttcagcatcg gcctggcgtt aacgcttgtc accgtaggcg
     9241 ttggcgcagc aatcagcgtt cagcaggtcg caaaacgctg gagcggattt aacactctcg
     9301 ctaaacgcgc tccctatttt tccagtctgt tgattggctt agttggtgtg tatatgggcg
     9361 tacatggctt catgggcata atgcgataac tcaatccttt aggcttgcat gttatcttca
     9421 gcccaggatg attcttaaat cagctatttc cgctgactct ctgctcgcta aggatgcttt
     9481 tagggcatcc tttcatttac actttttacg aaatcatggg atcactaaca aaatatcgct
     9541 tgtcagttat attgtatggc aggaaagata tgcgactgat attacagatc cccaaagtgg
     9601 agagtttatg accattaaaa ataagatgtt gctgggtgcg cttttgctgg ttaccagtgc
     9661 cgcctgggcc gcaccagcca ccgcgggttc gaccaatacc tcgggaattt ctaagtatga
     9721 gttaagcagt ttcattgctg actttaagca tttcaaacca ggcgataccg tcccagacat
     9781 gtaccgcacc gatgagtaca acattaagca gtggcagtta cgtaacctgc ccgcgcctga
     9841 tgccgggacg cactggacct atatgggtgg cgcgtacgtg ttgatcagcg acaccgacgg
     9901 taaaatcatt aaagcctacg acggtgagat tttttatcat cgctaaaaaa agccccctca
     9961 tcatgagggg gaaatgcaga caccttgtta ttttttatta ttcgccactt gctcgtcttg
    10021 cttattatta gtcgtatttc acattgatta atgcggttgc ctgtagtgca ccagatttta
    10081 ctttattttt atcataaaca tagtaactgg cagttactgg aattgagacc gttgtatctg
    10141 taatttttga cttcgccgca atttctttta aatttgtgta ccctttgctg ttatcagtga
    10201 tatttaaaaa tgtgactggc gaactattgc tttcaagcat aaatccgact ccattatctt
    10261 ctgcaccttt caggatcgtg ttactgccgg ctaacaaatc agaacttgat aaatacacct
    10321 gcatttttcg tgatgtttga ttttgttgac ctaataaatt tttacaacgc agaataatat
    10381 cccctttttt actatttatt gttgctttgt tacctgtggc tggtaattgt gaaacgggaa
    10441 tactctctaa attaattaat aaatcgtccg gtttgcaggt tgtggttatc gggttgtatg
    10501 acaatgccaa attaaaggaa tagacacctg cattttgtgc caatgcattt ctgtagctat
    10561 ttacacatgc atcccatgac ttgccaccta ataaacacag tagaaattgt actgcggcac
    10621 tcccgcgtgt cgatgatgaa gcactggata atgttgcaac accattaatc atcactgaac
    10681 cgccgccccc cgcagaaaca ttcacttttg cattactggc cgcaggtgtc accgttaccg
    10741 aataaccact ggtgaaactg gtctgattat ttgaca
//
LOCUS       DAZKGF010000169        10675 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZKGF010000169 DAZKGF010000000
VERSION     DAZKGF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.169"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            139..1506
                     /gene="dcuC"
                     /locus_tag="ACHOQV_004116"
     CDS             139..1506
                     /gene="dcuC"
                     /locus_tag="ACHOQV_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP9632402.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCIIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTLFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAIGAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1550..2047)
                     /gene="sspB"
                     /locus_tag="ACHOQV_004117"
     CDS             complement(1550..2047)
                     /gene="sspB"
                     /locus_tag="ACHOQV_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFP9632403.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2053..2691)
                     /gene="sspA"
                     /locus_tag="ACHOQV_004118"
     CDS             complement(2053..2691)
                     /gene="sspA"
                     /locus_tag="ACHOQV_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFP9632404.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3086..3478)
                     /gene="rpsI"
                     /locus_tag="ACHOQV_004119"
     CDS             complement(3086..3478)
                     /gene="rpsI"
                     /locus_tag="ACHOQV_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9632405.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3502..3930)
                     /gene="rplM"
                     /locus_tag="ACHOQV_004120"
     CDS             complement(3502..3930)
                     /gene="rplM"
                     /locus_tag="ACHOQV_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP9632406.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4149..5276)
                     /gene="zapE"
                     /locus_tag="ACHOQV_004121"
     CDS             complement(4149..5276)
                     /gene="zapE"
                     /locus_tag="ACHOQV_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFP9632407.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYDIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5470..5868
                     /gene="zapG"
                     /locus_tag="ACHOQV_004122"
     CDS             5470..5868
                     /gene="zapG"
                     /locus_tag="ACHOQV_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFP9632408.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6022..7389
                     /gene="degQ"
                     /locus_tag="ACHOQV_004123"
     CDS             6022..7389
                     /gene="degQ"
                     /locus_tag="ACHOQV_004123"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFP9632409.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7479..8546
                     /gene="degS"
                     /locus_tag="ACHOQV_004124"
     CDS             7479..8546
                     /gene="degS"
                     /locus_tag="ACHOQV_004124"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFP9632410.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8609..9547)
                     /gene="mdh"
                     /locus_tag="ACHOQV_004125"
     CDS             complement(8609..9547)
                     /gene="mdh"
                     /locus_tag="ACHOQV_004125"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP9632411.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9982..10452
                     /gene="argR"
                     /locus_tag="ACHOQV_004126"
     CDS             9982..10452
                     /gene="argR"
                     /locus_tag="ACHOQV_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFP9632412.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2637 a         2698 c         2649 g         2691 t
ORIGIN      
        1 ttttaatgaa aaatcacctc atctacaatt aaaaacacga catccgcacc ataaatagcc
       61 ttgcaaaaaa tataacatca ttgttttcaa tctgccgttt atgggattga ccattttctt
      121 ttgacacgga gttcaacaat gttcggcata attatatctg tcatcgtatt aattacgatg
      181 ggctatttga tcctgaaaaa ctacaaacct caggtggtgc tggctgccgc aggtatcttc
      241 ctgatgatgt gcggtgtctg gttagggttc ggtggtgtgc tcgatcccgc caaaagcagc
      301 ggctacttga tcgtcgatat ttataatgaa atcctgcgca tgctgtccaa ccgcattgct
      361 ggattggggc tgtcgattat ggcagtgggc ggttatgccc gctatatgga gcgcataggg
      421 gcaagtcgcg cgatggtgag cttgttaagc cgcccgttaa aactcattcg ctcgccgtat
      481 attattctgt cggcaactta cgtcatcggc caaatcatgg cgcagtttat taccagcgcc
      541 tccggtctgg gtatgttgct gatggtcacc ttatttccga cgctggtgag tctgggagta
      601 agtcgtctct ctgcggtggc ggttatcgca accaccatgt ccattgagtg ggggattctg
      661 gaaacgaact ccatttttgc tgcccaggtc gcaggaatga aaattgccac ttacttcttc
      721 cactaccagc ttccggtcgc ctcttgcatc attatctcgg tggcgatctc ccactttttc
      781 gtgcaacgcg ctttcgacaa aaaagataaa aatatcaatc acgaacaggc agagcaaaaa
      841 gctctcgata atgtcccgcc gctctattac gccattttac cggtgatgcc gttaatcctg
      901 atgctcggct cgctgttcct tgcccacgtc gggctgatgc agtcagaact gcatctggtg
      961 gtggtgatgt tactgagttt gacggtgacg ctgtttgttg agttcttccg caagcataac
     1021 ttgcgcgaaa caatggacga tgtgcaggcg ttttttgacg gcatgggtac gcagtttgcc
     1081 aacgtggtaa cgctggtggt cgcgggtgaa atatttgcga aaggcttaac gacgattggc
     1141 actgtcgatg cggttatcag gggggcggag cattctggtc tgggcggtat tggcgtgatg
     1201 attattatgg cgctggtcat tgccattggt gccattgtga tgggctcagg caatgcgccg
     1261 tttatgtcat ttgccagtct tattccgaat atcgcagccg gactacatgt accagcggtt
     1321 gtaatgatta tgccgatgca ttttgccacg acgctagcgc gcgctgtttc gccgattact
     1381 gcggtggtgg tcgttacgtc aggaattgca ggcgtttcgc cttttgcggt agtgaagcgg
     1441 acagcgatcc ccatggcagt cggtttcgtg gtgaatatga ttgccactat cacgctattt
     1501 tattaagcga ttaaaaagac aaaacaggcc gccagggcct gttttcattt tacttcacaa
     1561 cgcgtaatgc cggtcgacca ccgcgtggtg gctgcggagg ttcatcgtca ggatgagtgt
     1621 catcatcgtg atctggcttg tcgccatcaa taaccgacat aacggtttcg ttgtctgccg
     1681 atgcctcttc atcattcatg atgctggcat cttcatcgta ggcagcttca ggttcaaaca
     1741 tcgtgcctgc gccattttca cgggcgtaga tagccagcac ggcagccagc ggcacagaaa
     1801 cctgacgcgg aatgccacca aagcgcgcgt taaagcgcac ctcatcattc gccagttcca
     1861 gattgccgac agctcgcggc gcaatgttga gtacgatttg cccgtcacgc gcatattcca
     1921 taggaacctg cacgccaggg agcgtcacat ccaccaccag gtgcggcgtg agctggttat
     1981 ccagcaacca ctcatagaat gcacgcagca gatagggacg acgtggtgtt agctgtgaca
     2041 aatccataca gattaactcc ggcccagacg catttcacgt tcagcttcag ttaaagaagc
     2101 aaggaaagag tcacgctcaa agacgcgggt catatagcct ttcagctctt tcgcacccgg
     2161 gccgctgaac tcgatgccca gttgcggcag acgccacagc agaggagcaa ggtagcaatc
     2221 gaccaggctg aactcatcgc tcaggaagta cggcttctga ccgaagaccg gcgcaatcgc
     2281 cagcagttct tcgcgcagtt gcttacgtgc ggcatcggct tcagaagctg aaccgttgat
     2341 gatggtgttc atcagcgtgt accagtcttt ttcgatgcga tgcatgtaca gacggctttc
     2401 accgcgagct accgggtaaa caggcatcag tggcggatgc gggaaacgct catccagata
     2461 ttccataatg atgcgagatt cccacagggt cagctcacga tccaccaggg tcggaacgct
     2521 ctgattcggg ttgaggtcaa tcagatcctg aggcggattg tccttttcca cgtgttcgat
     2581 ctcgaaactt acacctttct cagccagcac aatgcggacc tgatggctat agatgtcagt
     2641 aggaccggaa aacagcgtca ttaccgaacg tttgttggca gcgacagcca tgaaaacctc
     2701 caggtatagt cagaattttt actgctacca gccaccaggt ggccagtcag aagttgtgtt
     2761 acccagtaag gaacgactct ctttgttcga aaatcaaaca aaaaatgagc aatacccgac
     2821 atttgggcag aaaattggat gatagtttac cagattttgc gacctttgtg gtgagtcgat
     2881 gccggaaatg gggaaaaaga gatgcgcttt agtctgaaat agttgactta gtcccttatt
     2941 ggcgatgtgg tttttgtttt acctgtctgt caggtggcag caaaaagcaa cttcccattt
     3001 tttacgctga tgcagatttt agccataaaa aaacccgccg aagcgggttt tttcgcaaat
     3061 tgttttctgc cggagcagaa gccaattaac gtttggagaa ctgcggacga cgacgtgctt
     3121 tacgcagacc gactttctta cgttcaacct gacgagcgtc acgagtaacg aagccagctt
     3181 tacgcagttc agaacgcagg gactcgtcgt attccatcag agcgcgggtg ataccgtgac
     3241 ggatcgcacc agcctgacca gagataccac cacctttaac ggtgatgtac aggtccagtt
     3301 tctcaaccat gtcgaccagt tccagcggct gacgaactac catgcgagca gtttcacgac
     3361 caaagtactg ttccagagaa cgttggttga ttacgatttt accgttgccc ggtttgatga
     3421 aaacgcgagc tgcggaactt ttgcggcgac cagtgccgta gtattgattt tcagccattg
     3481 cctataatcc cgattagatg attagatgtc aagaacttgc ggttgctgtg ccgcgtggtt
     3541 gtgctcgtta cccgcgtaaa ctttcagttt acggaacata gcacgaccca gcgggccttt
     3601 tggcaacatg cctttaaccg cgatttcaat cacacgctca ggacggcgag caatcatctc
     3661 ttcaaaggtc gcttgtttga taccaccgat gtggccggtg tggtgatagt acactttgtc
     3721 agtacgcttg ttgccggtta cagcaacttt gtcagcgttc agaacgatga tgtaatcacc
     3781 ggtatctacg tgcggagtgt attccgcttt gtgcttaccg cgcaggcgac gagccagttc
     3841 agtagccaga cggcccagag ttttaccggt cgcgtcaaca acataccagt cgcgttttac
     3901 ggtttctggt ttagctgtaa aagttttcat taaaagctta cccaataaaa agttacacgt
     3961 tggtgaacac ccaaacgtct tcaattgttg aggttcacac gacaaagtcc ggcaaaccta
     4021 ccccttcgaa tagcctatgc cagcacacaa aaagttttgg gaaaaaaact ttcttgtaac
     4081 gtggggtcgc aggattatag agaagtcggg gtcaaagatc gacccctttt tgtgatttgt
     4141 ggcaggtttt aacccgccaa atgctcgcgc ttcagatact cttcgctttg catctcttgc
     4201 agacgtgaca ggcaacgctg gaactcaaat ttcagccgct cgccctgata aatgtcatat
     4261 agcggcactt ctgcactcac cactaatttg acatggcgct cgtaaaactc atccaccagc
     4321 gcaataaaac gtcgcgcttc gctctccatc aaccgcgtca taactggtac atcaaacaac
     4381 atgactgtgt gaaagaggcg tgagagcgca atatagtcat gctgactgcg ggcgtcgacg
     4441 cacagcgtag taaaagagac cgccagcgtc tggttctcga cgcccattgt cgccagcggc
     4501 cgatggttga tttctaatgt cggtgaattt tctcgtttcg cccccgccag cgccaaccat
     4561 agtttatcca tttgcgcccg ggtttcatcg ttgaggggcg aaagccacag atgcgcctga
     4621 gtgagtgtac gcagtcgata atcaacacca gcgtccacgt tcattacatc acaatgctgt
     4681 ttaatggcat cgattgcagg caaaaaacgc gcacgttgta ggccatttcg ataaagttcg
     4741 tccggcggaa tatttgacgt cgctaccagg gtaataccgc gggcgaacag ggctttcatc
     4801 agaccgccaa gtagcatggc atcggtaata tcagaaacaa aaaattcgtc aaaacagagc
     4861 acgtcagttt ctgctttaaa gcgatcggca ataatttcca gcggatcggt ctgcccctgc
     4921 aaagcagtta gctcttcatg tacccgcagc ataaaacggt gaaagtgcag acgctgtttc
     4981 cgctctcccg gcaggctttg atagaaaagg tccatcagcc aggttttccc gcgtcctaca
     5041 ccgccccaca tatataagcc acgcactggc gtatgctttg tgtcttcgcg tttaccccac
     5101 agcttaccga cccgcgccat tagcccactc gtcctggggg ctggaggcgt gctattgatg
     5161 agttcctgat aaataatttc caggcggctg acggcctctt tttgaacgtc gtcgggttga
     5221 tggctgcctt cattgagcgc cttcaggtat tgcgatgttg gggtaacgct ttgcatgatc
     5281 ttattgttat tccttgaata atcggtgcgc cgttgttcac ggttgacgaa aaaaaggccg
     5341 ttctacacta cgcgatatgc agtcgggatt ccacttctgt ggaattaacg gttatagtgg
     5401 cataatcagc cgcaggcatg gagcctgaag ccaacaccct acggaaacaa aagacaacgg
     5461 gagatgttca tgacctggga atatgcgcta attgggttag tcgtcggcat cattattggt
     5521 gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc aacaggcgtt gcagtacgaa
     5581 ctggaaaaga ataaagctga actggacgag tatcgcgaag agctggttag ccactttgcc
     5641 cgcagcgcgg aattactgga taccatggcg cacgactatc gccagctgta tcaacacatg
     5701 gcgaaaagct ccagcagcct gctgccggaa ctgtctgctg aagcaaaccc gttccgtaat
     5761 cgtctggcag agtctgaagc cagcaacgat caggcaccgg tgcaaatgcc tcgcgactat
     5821 tctgaaggcg catccggcct actgcgtact ggcgcgaagc gcgactaatt tatttttcgg
     5881 gcgcagccat tgcgccctcc tcttctctcc ctccccgact atcatttaat ctggtgtctc
     5941 attgttagcc gtctgaaaat tcaataacat caaactgttt tgaatctctt ttcttatcat
     6001 tcaggtacga gagtaggaat aatgaaaaaa caaacccagc tgttgagtgc attagcgtta
     6061 agtgtcgggt taactctctc ggcgtcattt caggccgttg cgtcgattcc aggccaggtt
     6121 gccgatcagg cccctctccc cagtctggcc ccaatgctgg aaaaagtgct tccggcagtg
     6181 gtgagcgtac gggtggaagg aacggccagt cagggacaga aaatcccgga agaattcaaa
     6241 aagttttttg gtgatgattt accggatcaa ccggcacaac ccttcgaagg tttaggctcc
     6301 ggtgtcatca tcaacgccag taaaggctat gtgctgacta acaaccatgt gattaatcag
     6361 gcacagaaaa tcagtattca gctcaatgat gggcgcgagt ttgatgcaaa actgattggt
     6421 agcgatgacc agagcgatat cgccctgttg caaattcaaa acccgagcaa attaacgcaa
     6481 atcgctattg ccgactccga taaattgcgc gtcggtgatt ttgccgtggc ggtcggtaac
     6541 ccatttggcc ttgggcaaac cgccacctct ggcattgttt ccgcattagg ccgcagtggg
     6601 ttgaatcttg aaggtctgga aaactttatc cagacagatg cttccattaa ccgcggtaac
     6661 tccggcggtg cactgttaaa tcttaacggt gagttaattg gcatcaacac ggcaatcctt
     6721 gcgcctggtg gcgggagcgt cgggattgga tttgccatcc ccagtaatat ggcgcgaaca
     6781 ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg gtctgttagg catcaaaggc
     6841 acagagatga gtgccgatat cgccaaagcc ttcaaccttg acgtgcagcg tggcgcgttt
     6901 gtcagcgaag tgttgccagg ttctggctcg gcaaaagcgg gcgtcaaagc gggcgatatt
     6961 attaccagcc tcaacggcaa accgctgaat agctttgctg agttgcgctc tcgtatcgca
     7021 accaccgagc cgggcacgaa agtgaagctt ggcctgctgc gtaacggcaa accactggaa
     7081 gtagaagtga cgctcgatac cagcacctct tcgtcggcca gcgctgaaat gatcacgcca
     7141 gcgcttgaag gtgcaacgtt gagcgatggt cagctaaaag atggcggcaa aggtattaag
     7201 atcgatgagg ttgtcaaagg aagcccagct gctcaggcag gcttgcaaaa agacgatgtg
     7261 atcattggcg tcaaccgcga tcgggtgaac tcgattgctg aaatgcgtaa agtgctggcg
     7321 gcaaaaccgg ccatcatcgc cctgcaaatt gtacgcggca atgaaagcat ctatctgctg
     7381 atgcgttaat gtcgtaaacc gggcatcagg ctgacgtgtg atgtccggtt aactcgtggt
     7441 atgctgctgc cgttcccttt tttaatgacg cctccatcat gtttgtgaag ctcttacgtt
     7501 ccgttgcgat tggattaatt gtcggcgcta ttctgctggt tgccatgcct tcgctgcgca
     7561 gccttaaccc gctttccact ccgcaatttg acagtaccga tgagacgcct gccagctata
     7621 atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt ttacaaccgt ggtttgaaca
     7681 ccaactctca caaccagctt gagatccgca ccctgggatc cggtgtaatc atggatcaac
     7741 gcggttatat catcaccaat aaacacgtca tcaacgacgc cgatcagatc atcgtcgcct
     7801 tacaggatgg acgtgttttt gaagcgttac tggttggctc tgactctcta accgatctgg
     7861 cggtgctgaa aattaacgcc acaggcggtt taccaacgat tccgatcaat ccacgccgcg
     7921 taccgcacat tggcgatgtg gttctggcga tcggtaaccc gtacaacctc gggcagacca
     7981 ttacccaggg gattatcagt gccacgggtc gaatcggtct gaacccgacc gggcggcaaa
     8041 acttcctgca aaccgatgct tccattaacc acggtaactc tggcggcgcg ctggtgaact
     8101 cgctgggcga actgatgggc attaacacgc tgtcgtttga taagagtaac gatggcgaaa
     8161 cgccggaagg tatcggcttt gcgattcctt tccagttagc gaccaaaatt atggataaac
     8221 tgatccgtga tggccgtgtg atccgtggct acattggcat tggtggacgt gagatcgccc
     8281 cattacatgc gcagagtggc ggcatggatc aactgcaagg gatcgtggtt aacgaagtgt
     8341 cgcctgatgg cccagcggca aatgcaggca tccaggtcaa cgatctgatt atctctgtga
     8401 ataacaaacc cgctatttcc gccctggaga caatggatca ggtcgcggaa attcgccctg
     8461 gttcggtgat cccggttgtc gtgatgcgtg acgataaaca gttaacattg caggtcacca
     8521 ttcaggaata tccggcgacc aactaaggtc agcgtaaaaa aacaaaaacc ggagtctgtg
     8581 ctccggtttt ttattatccg ctaatcaatt acttattaac gaactcttcg cccagggcga
     8641 tatctttctt cagcgtatcc agcatacctt ccagcgcgtt ctgttcaaat gcgctcaggg
     8701 taccgataga tttacgctct tccacgccgt ttttacccag cagcagcggt tgagagaaga
     8761 aacgtgcgta ctgaccgtcg ccttcaacgt aggcacattc gacaacgcct tgttcgccct
     8821 gcagtgcgcg aaccagagac agaccaaaac gtgcagctgc ctggcccata gacagggttg
     8881 cagacccgcc accggctttc gcttcaacca cctcagtacc cgcgttctgg atacgtttgg
     8941 tcagatcagc cacttcctgc tcggtaaaac taacgccagg aacctgtgac agcagcggca
     9001 gaatggtaac accagagtga ccgccaataa ccggcacttc aacttcgcct ggctgtttgc
     9061 ctttcagttc cgcaacaaag gtgttggaac gaatgatatc cagcgtggta acgccgaaca
     9121 gtttgttttt gtcataaaca ccggcttttt tcagcacttc agcagcaatc gcaactgtgg
     9181 tgttaaccgg gttagtgata ataccaatgc acgctttcgg gcaggttttc gcaacttgct
     9241 gtaccaggtt tttcacgatg ccggcgttaa cgttaaacag gtcggaacga tccatacccg
     9301 gtttacgcgc tacacctgca gagataagaa cgacatctgc gccttccagc gccggagtcg
     9361 catcttcacc agaaaaacct ttgattttca cagcagtagg gatatggctc agatcgacag
     9421 ccacaccggg agtcactgga gcgatatcat acagagagag ttctgaacct gaaggcagtt
     9481 gggtttttaa cagtagtgca agcgcctggc caataccgcc agcagcgccg aggactgcga
     9541 ctttcatcct aaactcctta ttatattgat aaactaagat atgttgctcc gctgccgcga
     9601 ccttaatcca caaaattgcc gtttacaatg accacatctc aagaatgtgt agtcacgcaa
     9661 gtttagcgtt tatgcattta attgccgtaa tcaggaacct aacttacgta attaacagtc
     9721 gcttatgcat tagcgcaaca ttccaacagg tggtgacaat ataccctacc gttcagccaa
     9781 aacaacatca atttgataac aattaattta cttttaagca gaatttgcat gccgtgacgc
     9841 aggcatgttt ctcaataacg aaatttgata aaatcccgct ctttcataac attatttcag
     9901 ccttcttcag ggctgactgt ttgcataaaa attcatctgt atgcacaata atgttgtatc
     9961 aaccaccata tcgggtgact tatgcgaagc tcggctaagc aagaagaact agttaaagca
    10021 tttaaagcat tacttaaaga agagaaattt agctcccagg gcgaaatcgt cgccgcattg
    10081 caggagcaag gctttgacaa tattaatcag tctaaagtct cgcggatgtt gaccaagttt
    10141 ggtgctgtac gtacacgcaa tgccaaaatg gaaatggttt actgcctgcc agctgaactg
    10201 ggtgtaccaa ccacctccag tccattgaag aatctggtgc tggatatcga ctacaacgat
    10261 gcagttgtcg tgattcatac cagccctggc gcggcgcagt taattgctcg cctgctggac
    10321 tcactgggca aagcagaagg tattctgggc accatcgctg gcgatgacac catctttact
    10381 acccctgcta acggtttcac cgtcaaagac ctgtacgaag cgattttaga gctgttcgac
    10441 caggagcttt aatctctgcc ccgtcgcttc tggcggcggg gaaaatgttg cttttccctc
    10501 tcaccccccc tgctttctcc tgcgattaat ttaacgaata gtgcgtttta tcgcgacata
    10561 tcattcacat aatgaataca tgggttaaaa aaagcacatt atgcaaaatt cattatctaa
    10621 ttgaaaagaa tagaattaat aataaataat cgtgttttta attctttttt gttat
//
LOCUS       DAZKGF010000170        10484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZKGF010000170 DAZKGF010000000
VERSION     DAZKGF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.170"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(151..1098)
                     /gene="dusC"
                     /locus_tag="ACHOQV_004127"
     CDS             complement(151..1098)
                     /gene="dusC"
                     /locus_tag="ACHOQV_004127"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFP9632413.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPAGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMTISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(1160..2353)
                     /locus_tag="ACHOQV_004128"
     CDS             complement(1160..2353)
                     /locus_tag="ACHOQV_004128"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFP9632414.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARQGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEIARQRAVFTDHITMMDAVNAEEVVRIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWIGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDCADMPEDLRINAPVLWAGPCCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDAAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(2368..3012)
                     /gene="maiA"
                     /locus_tag="ACHOQV_004129"
     CDS             complement(2368..3012)
                     /gene="maiA"
                     /locus_tag="ACHOQV_004129"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFP9632415.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGIDYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLESHYPQVPLMPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEDKKRWYAHWIQQGLSAVEQLLRQSQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(3020..3721)
                     /locus_tag="ACHOQV_004130"
     CDS             complement(3020..3721)
                     /locus_tag="ACHOQV_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP9632416.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAIVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEN
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKASETGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGSVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(3737..4765)
                     /gene="gtdA"
                     /locus_tag="ACHOQV_004131"
     CDS             complement(3737..4765)
                     /gene="gtdA"
                     /locus_tag="ACHOQV_004131"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFP9632417.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGSLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTSMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNTLGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVTIGNETFSFSAKDIFVVPTWHGVSFQTTQDTVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(4777..6135)
                     /locus_tag="ACHOQV_004132"
     CDS             complement(4777..6135)
                     /locus_tag="ACHOQV_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9632418.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAIIRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAVCIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAVGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTQLITVVRQSTSVPDSLPHAGVV
                     NE"
     gene            6262..7170
                     /locus_tag="ACHOQV_004133"
     CDS             6262..7170
                     /locus_tag="ACHOQV_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9632419.1"
                     /translation="MANWAQKLKLHHLQMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGLTLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLKEMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYRVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            7301..7699
                     /gene="yohJ"
                     /locus_tag="ACHOQV_004134"
     CDS             7301..7699
                     /gene="yohJ"
                     /locus_tag="ACHOQV_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP9632420.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            7696..8391
                     /gene="yohK"
                     /locus_tag="ACHOQV_004135"
     CDS             7696..8391
                     /gene="yohK"
                     /locus_tag="ACHOQV_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFP9632421.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            8521..9405
                     /gene="cdd"
                     /locus_tag="ACHOQV_004136"
     CDS             8521..9405
                     /gene="cdd"
                     /locus_tag="ACHOQV_004136"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP9632422.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALVAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            9555..10274
                     /gene="sanA"
                     /locus_tag="ACHOQV_004137"
     CDS             9555..10274
                     /gene="sanA"
                     /locus_tag="ACHOQV_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFP9632423.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            10277..>10484
                     /locus_tag="ACHOQV_004138"
     CDS             10277..>10484
                     /locus_tag="ACHOQV_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFP9632424.1"
                     /translation="MDVQQFFVVAVFFLIPVFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSI"
BASE COUNT         2379 a         2902 c         2723 g         2480 t
ORIGIN      
        1 taacatccct gtaaaacgcg ccctcagccc acatccatgt gggctgcccc ggccttcagg
       61 gaacgcctcg gcgattttga cgccaccaaa caaccgtgcg gcctattgat aaagagctaa
      121 cacattgtca aaaaacatca ctatggtttc ttagagtttc tcgatatcaa ttgcctgaat
      181 agcccttgca atatcagggg aattattcaa cacccgaaca tgctgaaata attccgttgc
      241 ttcatcgtat tctttacgca aataactcaa ccactgttta atccgcgcaa cgtgatataa
      301 cccggtatcg ccctgctttt ccagacgggt atatttttgc agcaaagcaa ctacctccgg
      361 ccacggcatt cgcggttcgt tatattttac cacccggctc aggttgggaa tattgagcgc
      421 cccgcgacca atcatcactg cgtcgcatcc gctgatcgtc atgcattgtt gcgcactctg
      481 ccagtcccag atttcaccgt tggcaatcac aggaatattc aaccgctggc gaatctcgcc
      541 aatcgcctgc cagtcaatat gctccgcgcg gtaaccctgc tcttttgtcc gcccatgtac
      601 taccagctcc gtagcgccag cctgttgcac cgcatcggcg atttcaaatt tcttctcacc
      661 gctgtcccag cccagacgca cttttacgct gacaggcaaa tgcgccggta cagcttcgcg
      721 catcgctttt gcaccctgat agatgagttc aggatctttg agtaacgtcg ccccaccgcc
      781 gctaccgtta accgttttcg acgggcagcc gcaattgaga tccacgcccc aggaacctaa
      841 ctcgaccgca cgggcggcat tctctgccag ccattgtggg aactgaccta acaactgcac
      901 gcgcaccagc gtaccggctg gtgtccggct ggcgttttgt agctcagggc aaatgcgatg
      961 aaagactttt accggcagca gttgatccac cacgcggaca aactcggtga tgcacagatc
     1021 gtagtcgtta acttcggtca gcaattcacg caccagagag tcgagtacac cctccatcgg
     1081 tgccagtaac acacgcatat catcacccgc aaaaaaatga ggcgctatgt tagcgcctat
     1141 ggtcagcgga ttaaagtact cagcgtggtt gcaaacagtt atcttctttc cagccgtaca
     1201 gccactccat gccgcggtaa aactctgcct gcgatttccc tttccacaac aggttacgta
     1261 cctggcgctc tactcctgcg gcatgataaa cccgccccat ttcacgggtc gaccagacga
     1321 tgcgcgcggt gcgcggaata cgtaccgact catacagcgc aaaggcttgt gccgcatcac
     1381 cctcacactg cgccagcgcc ttacccagtg ttaccgcgtc ttccagcgcc atacaggcac
     1441 cctgcgccat atactgcgcc accggatggg ccgcatcgcc caccagcgta atgcgcttcg
     1501 ttccccactt cgctaccggt tcacggtcgg cggttgacca gcgtcgccag gaggtcggtt
     1561 tatccagcat ctggcgcgga cgggggtgaa tcccttcaaa gtaagagagc acttcctctt
     1621 tactgccatc cttcacgccc cactcttcct gctggcggct gtggaatgtc accaccaggt
     1681 tatactgctt gccgccgcgt aaagggtaat gcaccaagtg acagcacggc cccgcccaca
     1741 gtactggtgc gttaatgcgc aaatcttccg gcatatcggc acaatcgata acagcacggt
     1801 agaccacatg cccggtgaca cgcggcgcat cgcccaataa gctctggcgc accactgact
     1861 tcacgccgtc acagccaatc agaatatcgc cgatccaact attgccgcga tcgtcgaaaa
     1921 cggtgacatc gtcttctgtc tcgcgaatat ccacgacgtt ggtggaggtg tgatattcca
     1981 cgtccggatg cgtcagtacc gcttcccaca ccgaggcgtg aatatcaacc cggtggatca
     2041 ccgcgtaagg gccgccgaaa tgatcgcgaa aggcctgacc ggtttcaatg cgcaccactt
     2101 cttcggcgtt taccgcatcc atcatggtga tgtgatcggt gaataccgcg cgctggcggg
     2161 cgatttcgcc gacgccgagg ctatccaacg ccgaaaacgc atttggcccc aactggatac
     2221 ctgcgccaat ctcaccgatc tcatgcgcct tttccagcag caccactttg atcccctggc
     2281 gggccagtga caatgcagtt gccgcaccgc cgatcccgcc tcccacaatt actgcgcgtg
     2341 tcactttagt cattatggtt ctcctggtca ggctgaaatt ttatcttgtt gattttctgg
     2401 cgctgcggca atgaacgctg gcagctgcgt gcaggcgtcg tataccgctt tgcagcgcgg
     2461 atacccgctc agatcgcagt tcatacgtag cgcgttcgcc cactgtggaa caaggcagca
     2521 atctgccagc gtcggcgtct cgcccacaca aaattgcccc gactggctct ggcgcagtag
     2581 ctgttccacc gcgcttaacc cttgctggat ccagtgcgca taccagcgtt ttttatcttc
     2641 ttcactgacg ttcagctctt cggtcaggta gcgcagcacg cgtaggttat tcaatggatg
     2701 aatatcgcag gcgatggcat agacaatttc cagcaccttg ttgcgggccg gttcttcctg
     2761 cggcatcagc ggaacttgcg ggtagtgact ctccagccag tcaataatcg ccagcgattg
     2821 tccgagtgat tcgccctcgt cggtcagcag cgtcggcacc agcccgaccg gattcatccg
     2881 ccggtaggcc agttcgtttt gctgaccaat gcggatattc accccttcgg tctggtaatc
     2941 gatacccttc agcgccagcg caatgcgcac ccggtaagac gctgaactat taaaaaaact
     3001 gtacagcttc atcatcacct cagacaatct tcaccgcgat aggcgttaag ccctcgacgt
     3061 tgcccgtgat cacatcacct ttgactactg agccaacccc ttccggcgta ccggtaaaga
     3121 ttagatcgcc cggttgcagt tcaaagaagc ctgacaggta gctgatggtt tcattcactg
     3181 accagatgag atgacgaata tcgctgcgct gatgatcttc gccgttaacc tgtaaccaga
     3241 taggtgcgtt atcaataccg ccagtttcgc tggctttgtg cagcggcgcg atgggggccg
     3301 agagatcgaa cgctttgccg atttcccacg ggcggcccat ctggcgcatt tccatttgac
     3361 ggtcacggcg agtcatatca agacccgtgg cataacccca gacatattca tgggcgtttt
     3421 ccagcgggat atcactgcct tttttaccaa tcgccaccac cagttcaatt tcataatggt
     3481 agttatcggt ctgcgccgga tacggcagcg ccagcgtttc gccagcggca accggaacaa
     3541 tggcatccgc cggtttgcag aagaagaatg gcggttcacg atccggatca aagcccattt
     3601 cgcgggcgtg ggcggcatag ttacgtccga cgcagtaaac gcggcgtacg gggaattgtt
     3661 catcactgcc aacaaccggt acggtaacgg gcgcttgtgg ttcgaatacg tattgagtca
     3721 ttttctctct cccaaattaa taacgtgctt cgcggaacag ccccagggct tcttgtaccg
     3781 gtctgtccga aaaactaaat aaaacggtat cttgcgtggt ctggaacgac acgccgtgcc
     3841 aggtcggcac cacgaagata tcttttgcac taaagctgaa ggtttcattg ccgatagtga
     3901 cctgcccgct accttccacc acatggtaga tggtgctgtc agtggtacgc gccacgcgtg
     3961 aggcaaaccc tttcggcagc agttgcagaa aagcgcccat cgacggcatc ggatagccac
     4021 cggtgaccgg gttgacgtag cgcattttgt agccatccca ctcatcggca tcgcccaggc
     4081 gggttaaatc gtgtagcact tcgcggctac ggtcataacg gtagttaaag atgggcgagg
     4141 agttcccggt ctgatggcgc aacggcaaca tattggcagc gtaacgcggc aggtaatcgc
     4201 cctctttacg cgttactggt tgttgctcct ccgggtaatc ttcggcaaaa ccgcagccca
     4261 gagtgttcac taatggcaga tccagcccat cgagccagat aaccggttcg tcgccagggt
     4321 taccgtgatc gtgccagcgc cactgcgggg tcaggataaa atcgccctca ttcattgacg
     4381 tgcgttcgcc atcaacggcg gtgaatgccc ctttaccttc aacaataaaa cgcagcgccg
     4441 actggttatg gcgatgactc ggtgccactt cgcccggcat gatcagttgc aaaccggcat
     4501 ataaagtcgc ggtgatggaa gactgcccgc gcagcgccgg gttttccagc accagcacgc
     4561 ggcgcacggc ttctttcgcg ccaatcaggc tgccgctctc cagcagcaac gggcggatct
     4621 cctgataatt ccagtacgct ggcgcacagt tagcgttggg cgtttttggc accagatggt
     4681 gcagtgactc ccacagcggg gtcaggtttt gcccggaaat atgctgataa aattgctcgc
     4741 ggctattttg attgttatca gtcattggga tgctccttat tcgttcacaa cacctgcgtg
     4801 aggcagtgag tcagggacag aggtcgactg gcgaaccacg gtgatcagct gggttaacat
     4861 caccgcactg atcgccgcgg gcacagcgat aatgaaaaac agggtgtcga aagagaaatt
     4921 catcgccatc ataacgccgc cagacagcga gccaacaatc gcgccgcagc ggcccacggc
     4981 gtttgaccag ctcacgcccg tggcgcgact ttgtgttgga taaagtgttg cggtcagcgc
     5041 attcagcccc acctgcgatc cgctgatgcc aatgccggtg ccgaaaatcg ccagcgccat
     5101 tagccataat ccgtcctggc ttaagccaat catcacgatg catacagcgc ctatggcata
     5161 gctcagcgcc agtacgcgga aaggattgaa cttatccata agcacgccca gcgccagcgc
     5221 gcccaacgta ccgccaatct ggaacgcggc ggtaacccag gaggcatgtt gcaggtcgat
     5281 gccgcgatgg ttgagcagcg ttggcatcca gcttgagagc aggtaaataa tcagcaagct
     5341 cataaagaac accacccaca gcatcaacgt gatgggtaac tgacgcccca taaacagttg
     5401 acgaatgctg ccttttgtaa cggaggctga ttcaatgaga taaaagtggg tatcgacata
     5461 acgctcgcgg atgatggcgc tgacggtttt cgcaatcacc gcctggggta gctggcggcg
     5521 cacctgccag cgtggcgatt ccggcagaac caccagcagt gcaacaaaca gcatcagcgg
     5581 taatacgcca cccaacacca ggatcccgtg ccagccaatc accggcacca gctgcgcgct
     5641 gacaatcccg ccaaacgccg aacccagggt aaagccgcaa aacatcagcg tcaccagcgc
     5701 gccgcggcgg cgggcaggca gatattccga ggtcatggta attgtatttg gcatcgcgcc
     5761 acccagccca aggccggtga ggaaacgcag gaataccagc gtttgcagat ccggtgagaa
     5821 agccgaggcg aggcttaacg cgccaaacaa aaagacgcac agctcaatca cccgtttgcg
     5881 gccaaagcga tcggacaacg gaccacaaag cagtgcgcct gcggttaacc ccagcagacc
     5941 cgcgccaaaa agcggggcga gatcgcctgc ggtgagttgc cagtgagtgc gaatatcagg
     6001 ggcgataaag ccgatagccg cggtgtcaaa accatcgagc attaccacca gaaaacagca
     6061 gatgataacg cgccactgca tcttgccgac cggagcagca tcgatcagtg cttgtaaatc
     6121 acgtcgttga gtcatagtaa atgcctcagt gcaggtaagg tagatgtttc atttgtttta
     6181 tgtatgttgc gatattgtga ttaaagagat gaaatgtaca atggcatttc ggacagagag
     6241 ataacctgga ggttatggca aatggcaaac tgggcgcaga aactaaagct tcatcacctg
     6301 caaatgctgg tggcgctggg tgaacagggc aatctgaccc atgtcgcgcg gatgatgaat
     6361 atcacccagc ctgcgctgtc gaaatggttg tcacaactcg aagatgagat cggcctcaca
     6421 ctctttgagc ggcacagcaa aggacttcgt cctactgaag gcggcaaatt gttattgcaa
     6481 catgcccaac ggttaatcaa tgacatggag cgctcgcaac atgaactggc ccgttttaaa
     6541 caaggtggac tggtcggcag tctgaaaatt ggctgttctc ccgtcgccac tgactgcgta
     6601 tcccaggcga ttttgcattt gctcaaggag atgccaactc tacacctgaa tatcgaagag
     6661 aaggtgatga ccccattgct gcaagatctg ttggcaggtt ctgtcgatgt ggtggttggg
     6721 cgaattggcg gtcgcgcgtt gcagctgccg ctgaattatc gcgtgctcta caccgagccg
     6781 gtgtgttttg tcgcccgtcc tgagcatcca ctggcgaaat acgccaccct cagctggaat
     6841 gatcttgcta actggcggtg gatagtctgg ccgacaggta cccccatccg catcagcatt
     6901 gataatgcgc tggtagacca cggcgtgatg ctgccagaaa acacgattga gtcggcgtcg
     6961 atgaacgtca gtaccaacct gttacaaagc agcgatatga tctctatttt gtcattacgc
     7021 ctggcgcaac gttatgccag ccagggacaa ctggtgattc tcaacctgcc aaagatagaa
     7081 cagaaaggca gcgtcggtgt gttctggcgc aataacgaaa cgccttctta tgctttaagt
     7141 cagtttctgc actttttaca gcagaattag tgtgctgaac tgggcgctcg cccgaggtta
     7201 cgtccctaaa attcaactat gctgagattt cttaatgtga tcggtagcac gttttacgaa
     7261 ttaattgtat gatgaatcca tctcatctgg ggtgttgatt atgagtaaga cactgaacat
     7321 tatctggcaa tatttacgtg cttttgtcct gatttatgcc tgcctgtatg caggcatttt
     7381 cattgcttcc ctgctaccgg taaccattcc gggcagcatc atcgggatgc tgatcctgtt
     7441 tgtcctgctg gccttgcaaa ttcttccggc gaaatgggtc aatccggggt gctacgtact
     7501 gattcgctat atggcgctat tgtttgtgcc gattggtgta ggcgtcatgc aatattttga
     7561 tttgctccgc gcacagtttg gtccggtagt ggtttcctgt gcagtcagta cgctggtggt
     7621 ttttctggtg gtgagctgga gttcacaact ggtgcacggt gaacgtaaag tcgtaggtca
     7681 gaaaggatca gaagaatgat ggcgaatatc tggtggtcat taccgctgac gttgattgtc
     7741 ttttttgccg cccgcaaact ggcggcacgg tataagtttc ccctgcttaa cccgttgctg
     7801 gtagcaatgg tggtgatcat tcctttttta atgctgaccg gcatctctta cgacagctac
     7861 tttaaaggta gcgaagtgct aaacgatctg cttcaaccgg cggttgtcgc actggcctat
     7921 cctttatatg agcagctgca ccagatccgc gcgcgctgga aatcgatcat caccatctgt
     7981 tttatcggca gcgtggttgc gatggtgacg gggacttctg tggcattgtt gatgggcgct
     8041 tcaccggaaa tcgccgcgtc aatcctgccg aaatcagtca ccacgcctat tgcaatggcg
     8101 gttggcggca gtattggcgg tattccggca atcagcgcag tttgcgtgat tttcgtcggc
     8161 atcctcggcg ctgtgtttgg ccatacattg cttaatgcga tgcgtattcg taccaaagct
     8221 gcgcgcggtc tggcaatggg cacagcctcg cacgccctcg gtacggcgcg ctgcgccgag
     8281 ctggattatc aggaaggtgc atttagttcg ctggcgctgg tgttatgcgg gattattact
     8341 tcgctgatcg caccgttcct tttcccgatt attctggcag taatgggcta aaatttgcga
     8401 tgcgtcgcgc atttttgatg taagtttcac gcgttgcata attaatgaga ttcagatcac
     8461 atataaagcc ataacgggtt cgtaaactgt tatcccatta catgattatg aggcaacgcc
     8521 atgcatccac gttttcaaac cgcttttgcc caacttgcgg ataacttgca atctgcactg
     8581 gaacctattc tggcagacaa gtacttcccc gctttgttga ccggggagca agtctcatcg
     8641 ctgaagagcg caacggggct ggacgaagac gcgctggcat tcgcactact tccgctggcg
     8701 gcagcctgtg cgcgtacgcc attgtcgaat tttaatgttg gcgcaattgc gcgcggtgtg
     8761 agcggaacct ggtattttgg tgccaatatg gaatttattg gtgcgacaat gcagcaaacc
     8821 gttcatgccg aacaaagcgc gatcagccac gcctggttga gtggtgaaaa agcgcttgta
     8881 gccatcaccg ttaactatac gccttgtggt cactgccgtc agtttatgaa tgaactgaac
     8941 agcggtctgg atctgcgtat tcatctgccg ggccgcgagg cacacgcgct gcgtgactat
     9001 ctgccggatg cctttgggcc gaaagatctg gagattaaaa cgctgctgat ggacgagcaa
     9061 gatcacggct atgcgctgac gggtgatgcg ctttctcagg cagcgattgc ggcggcaaac
     9121 cgttcgcaca tgccttacag taagtcgcca agcggtgtcg cgctggaatg taaagacggt
     9181 cgtattttca gcggcagcta cgctgaaaac gccgcattca acccgactct gccaccgttg
     9241 cagggggcgt taattctgtt gaatctcaag ggttatgatt acccggatat ccagcgcgcg
     9301 gttctggcag aaaaagccga tgcgccgttg attcagtggg atgccacctc cgcaacactg
     9361 aaagctctcg gctgtcacaa tatcgaccgc gtgcttctcg cttaagcctg gtgccggatg
     9421 cggcgtgaac gccttgtccg gcttgccagc cctctcctgg tgtcgaaaat cccggcaaac
     9481 agtttgccgt ttcttgcgca aaaccagcgg gtaaagtagc ctgatggaaa ttttccttag
     9541 atcgagtctc ctgcatgtta aagcgcgtgt tcctcagcct gttagtcctg atcggcttgc
     9601 tgctgttgac tgtgctcggc ctcgatcgct ggatgagctg gaaaaccgcg ccttatatct
     9661 acgacgaatt gcaggatctc ccctaccgcc aggtcggtgt ggtgctcgga acagcaaaat
     9721 attatcgtac tggcgtaatt aatcagtatt atcgctaccg cattcaagga gcgattaatg
     9781 cctataacag cggtaaggta aattatctat tactgagcgg cgataacgca ttgcaaagtt
     9841 ataatgagcc gatgaccatg cgcaaagatt taatcgctgc tggtgtcgac ccatcagata
     9901 ttgttctcga ttacgcaggc tttcgtacgc tggattccat cgtgcgtaca cgcaaagttt
     9961 tcgatactaa tgatttcatt attatcaccc aacgtttcca ctgtgagcgg gcattattta
    10021 ttgcgctgca tatggggatt caggctcagt gttatgccgt accgtcaccg aaagatatgc
    10081 tgtcagtacg tattcgcgaa tttgccgccc gtttcggtgc gctggctgac ctttatattt
    10141 ttaaacgtga accgcgtttt ttagggccgc tggtccctat tccggctatg caccaggtgc
    10201 ctgaagatgc gcaggggtat cccgccgtca cacccgaaca gttacttgaa ttacaaaaga
    10261 aacaaggaaa gtagttatgg atgtacagca gttttttgtc gttgccgttt ttttccttat
    10321 cccggtattt tgtttccgcg aagcatggaa aggctggcgc gcaggcgcga ttgataaacg
    10381 ggttaaaaat gcaccggaac cggtgtatgt ctggcgagca aaaaatcccg gactcttctt
    10441 cgcttatatg gtggcatata tcggcttcgg aattttatct atcg
//
LOCUS       DAZKGF010000171        10459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZKGF010000171 DAZKGF010000000
VERSION     DAZKGF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.171"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..478
                     /locus_tag="ACHOQV_004139"
     CDS             <1..478
                     /locus_tag="ACHOQV_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9632425.1"
                     /translation="VVVTFTGILLAKSCDITTGSKDQSVKMGTYDANEFLNVGDVSPS
                     QTFTINVQGCPTAKSTVYSSGVSANVRFTGDADAINSALLRLSAGADSATGLGIEILD
                     NNDVAIAINGESGFRDLVLNENGDANLTFKLRYKSTQENVHAGQANALLYFDIDYQ"
     gene            429..1001
                     /locus_tag="ACHOQV_004140"
     CDS             429..1001
                     /locus_tag="ACHOQV_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9632426.1"
                     /translation="MPGKQMLCCILISIISEGDMKIFISLFLFIISTNSFADDITHAG
                     VVRIEGLITEKTCIISDESKNFTVNMPDVPSSSVRSAGDVTEKVYFSITLTRCGSDVG
                     NAYIKFTGNTVSEDASLYKLEDGSVDGLALTIFDKNKGSISNDVKSIGFSLTSSVDNI
                     LHFFAAYKALKNNVQPGDANASVSFIVTYD"
     gene            967..1746
                     /locus_tag="ACHOQV_004141"
     CDS             967..1746
                     /locus_tag="ACHOQV_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9632427.1"
                     /translation="MRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSS
                     ISVELKNTSDRDMLVKVAVSPDDARQLTGTVESLPSFSGTVFIATPPLFVLKPDKNTK
                     IRINRVGGSLPADRESLFILNVAALPSLENSHPTKTDNQLQIAVRNRMKIFYRPSNLS
                     EDPNISYQKLRWARKNEIVTVYNPGPRYVTLYNLHVDGKVMDGGMIAPFSHRQQSWCK
                     AQGICEITWQTLDIYNNVLPVWKVNMNPLQMDVSGSQFKTD"
     gene            1761..2882
                     /locus_tag="ACHOQV_004142"
     CDS             1761..2882
                     /locus_tag="ACHOQV_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001588797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9632428.1"
                     /translation="MITYAGYKVKIIFIIFLFLGYHVVAYAEMGRDREFCYPGTPGNN
                     TTPATFFYDFGTVIISDVNNNSAGTVLPSQNWKIGRYKAYCNSLDDYEVYFSGISGID
                     PSGASGNPQGAEIFIPVTHEISISTHIKLYNQNGTMTDKTVPFENYSTNYPADRSKAD
                     YWSSGTEGYIKIRLDKKIISDVSLNNVLLVSLYASQRQTEHGPVPLFNAWISNLDIQV
                     PQGCTINEGTSFTVNMPDVWASELSRAGAGAKPAGVTPVATTIPINCTNKDTDAVMTL
                     VFDGNISATRDTNGKQSIIQAQDNPDVGIMIMDSQQNSVDLNALATSVGVPFRLVENQ
                     AASAQTADVTFLALPVSTTGKVPAAGRYNALAVLRVEYQ"
     gene            3056..3766
                     /locus_tag="ACHOQV_004143"
     CDS             3056..3766
                     /locus_tag="ACHOQV_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632429.1"
                     /translation="MELVDTLFASLSGTDPFTGVDITIANCKSTYWDEGIIQQLINQV
                     LDEGEKFAGTVGLEGLSRYDVTLNIGLTSSNVWPGFSLDTATIGRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDFTVHFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKEAW
                     KFHTDNSLRGFRDKQAELKLYARHYSVENCTEESCRDCNYCIRPSFSLSRSSIIRLNA
                     ANARFVYQPFTRDQRKRG"
     gene            3769..4545
                     /locus_tag="ACHOQV_004144"
     CDS             3769..4545
                     /locus_tag="ACHOQV_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632430.1"
                     /translation="MQPSSAMQALIEQVYHIFRRYPVPQQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDERAILQRFACQYMADWVSGDEAVELQYMLEMFSRADIALSPLLDAIIS
                     VPGYQPTVSMACLLWHNREENIQNSRFEQDDDDKAITAQINAWAFNNQFILKERARQA
                     IENLLNQPEQGTHYQVWEDDWMIDECLCAMYDASSESPGK"
     gene            4548..5153
                     /locus_tag="ACHOQV_004145"
     CDS             4548..5153
                     /locus_tag="ACHOQV_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632431.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMGGIGWQFSRRTPPELKNRLHYWLVFSPAY
                     FWLILSNFILYITPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSQGQKWVEWGTCLSLAILAQGPLFNYLQ"
     gene            complement(5207..6712)
                     /gene="glpD"
                     /locus_tag="ACHOQV_004146"
     CDS             complement(5207..6712)
                     /gene="glpD"
                     /locus_tag="ACHOQV_004146"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFP9632432.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNAEQQSRVSQWLVEYTQQKLSLAS"
     gene            6902..7228
                     /gene="glpE"
                     /locus_tag="ACHOQV_004147"
     CDS             6902..7228
                     /gene="glpE"
                     /locus_tag="ACHOQV_004147"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFP9632433.1"
                     /translation="MEQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWRRQFPA
                     EVAYGA"
     gene            7273..8103
                     /gene="glpG"
                     /locus_tag="ACHOQV_004148"
     CDS             7273..8103
                     /gene="glpG"
                     /locus_tag="ACHOQV_004148"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFP9632434.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            8120..8878
                     /locus_tag="ACHOQV_004149"
     CDS             8120..8878
                     /locus_tag="ACHOQV_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFP9632435.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(8860..10458)
                     /gene="rtcR"
                     /locus_tag="ACHOQV_004150"
     CDS             complement(8860..10458)
                     /gene="rtcR"
                     /locus_tag="ACHOQV_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFP9632436.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVINRL
                     ELLHDARSRSLFETLKCDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PDKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEMNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLSQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITREVVEDEINRLRYNWQES
                     RPSAITALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
BASE COUNT         2645 a         2391 c         2588 g         2835 t
ORIGIN      
        1 agttgttgtt acatttactg ggattttgtt ggcaaaaagc tgtgacatta ctaccggaag
       61 taaagatcag tcggtaaaaa tgggcactta tgatgctaat gaatttttaa atgtaggtga
      121 tgtttctccg agtcaaacgt ttacgattaa tgtacaagga tgcccgactg caaaaagtac
      181 tgtttatagt tctggtgtga gcgccaatgt gcggtttact ggtgatgcag atgccattaa
      241 tagcgcatta ctgcgtctct ccgccggtgc agatagtgcg actggactgg gaatagaaat
      301 attggataac aatgatgtgg cgattgctat taatggtgaa tcaggttttc gtgatttagt
      361 gttaaatgaa aacggtgatg ctaatttgac gtttaaactt cgctataaat caactcagga
      421 aaatgtacat gccgggcaag caaatgctct gttgtatttt gatatcgatt atcagtgagg
      481 gagatatgaa gatattcatt agtttatttt tgtttataat atcaacaaat tcttttgctg
      541 atgatatcac tcatgccgga gtggttcgta ttgaagggct aattaccgaa aaaacctgca
      601 ttatttctga tgagtcaaaa aattttacag ttaatatgcc agacgtaccc agtagttcgg
      661 taaggagtgc aggggatgtt actgaaaagg tttatttttc cataacgtta acccgctgtg
      721 gtagtgatgt tggcaacgcg tatataaagt ttaccggcaa tacagtttct gaagatgcca
      781 gtttatataa gctggaagat ggctcggtag atgggcttgc actcacgatt tttgataaga
      841 acaaaggcag tattagtaat gatgttaaaa gcataggttt ttcacttaca tcatcagttg
      901 ataatatatt gcattttttt gcggcttaca aagcattaaa aaataatgtc caaccagggg
      961 atgccaatgc gtcagtatcg tttattgtca cctatgatta aggtagtcat agtattattt
     1021 tcccttttat tctctacagc acaagcagga ttagtgatcg gtggaacgcg ttttgcgtat
     1081 ccggaaaatc agtcctcaat atctgttgaa ctcaaaaaca cttcagacag agatatgtta
     1141 gtcaaggttg cagtctcccc tgatgatgct cgtcagttga cgggaacggt tgaatctttg
     1201 ccctcatttt ctggaacggt atttatcgca actccgccac tgtttgtttt gaagcctgac
     1261 aaaaatacaa aaataagaat taatcgtgtt ggtggcagtt tacctgccga ccgggaaagt
     1321 ttatttatac ttaatgttgc tgcattacca tcgttggaaa atagtcatcc aaccaaaact
     1381 gataatcaat tgcaaatagc ggtccgcaat cgtatgaaaa tattctatcg gccatcaaat
     1441 ttgtctgaag acccgaatat ttcatatcaa aaattacgct gggcacgaaa aaatgaaatt
     1501 gttaccgttt ataatccagg ccccagatat gtgacactct ataaccttca tgttgatggt
     1561 aaagttatgg atggtggtat gatcgcacca tttagccatc gtcaacaatc atggtgtaaa
     1621 gcgcaaggga tttgtgaaat aacatggcag acattggaca tttataataa tgtattgcct
     1681 gtatggaaag taaatatgaa tccgttgcaa atggatgtgt caggctcaca atttaaaacg
     1741 gattagtaaa aggaaatcgt atgattactt atgccggata taaagtgaaa attattttta
     1801 taattttttt attcttaggg taccatgtcg tagcctatgc tgaaatgggc cgggatcgtg
     1861 agttttgcta tcctggtact ccaggaaaca atactacgcc agcgaccttt ttttatgatt
     1921 ttggcacagt tattatttca gatgttaaca acaattcagc cggtactgtt ttaccatctc
     1981 aaaactggaa aataggtcga tataaagcat actgtaattc tcttgatgat tatgaagttt
     2041 atttttcagg tatttcagga atagatcctt caggtgcttc aggtaatcca cagggagcag
     2101 aaatatttat tcctgttacg catgaaatat ctatttcaac acatataaaa ctctacaatc
     2161 aaaatgggac gatgacagat aaaacagtac cattcgagaa ctatagtact aattatcctg
     2221 ctgacagaag taaagcagat tactggtctt caggaacaga aggctatatt aaaattaggt
     2281 tggataaaaa aatcatctct gatgtctctc tgaataatgt acttttagtt tcattgtatg
     2341 ccagtcagcg gcaaacggaa catgggccag taccattatt taatgcctgg atcagtaacc
     2401 tggatattca ggttccgcaa ggttgcacta ttaatgaggg tactagtttt actgttaata
     2461 tgccggatgt gtgggccagt gaattgagcc gggctggtgc tggagcgaag cctgctggtg
     2521 ttactcctgt agcaacaact attccgatta attgtacgaa taaagataca gatgcggtaa
     2581 tgacgttggt attcgacggt aacatttcag ctacacgtga taccaatggg aaacaaagta
     2641 ttattcaggc acaagataat cctgatgttg gtattatgat tatggatagt cagcaaaact
     2701 ccgtagattt aaatgctctg gcaacatcag taggcgttcc gttcagattg gtggaaaacc
     2761 aggctgcttc tgcgcaaact gctgatgtta ctttcctggc tttacctgtt agtacaactg
     2821 gaaaagtgcc tgcggcaggt cgttataatg cactggctgt attgcgagtg gaatatcagt
     2881 aatttttgag ctagtacaga atcggtttag ccatcaccgc aaaataaaat gcaataatag
     2941 ggatgaacag aatgtaaaac aaaaaaatat ttttaattct acagggacaa aatctggtgg
     3001 aaaatgtagg ttatgtgccg atggataaaa tgttgcatta agccggataa taaaaatgga
     3061 attagttgat acgttatttg caagcctctc cggtactgat ccattcactg gcgtggatat
     3121 tacgattgcc aactgcaaat caacatactg ggatgaaggc attattcagc aactcattaa
     3181 ccaggtcctg gatgaagggg aaaagttcgc cggtactgtc ggtcttgagg gattatctcg
     3241 atatgatgtc acattaaata ttggcttaac ctccagcaat gtatggccgg gattttccct
     3301 cgataccgcc acgatcggtc gtctttgtgc ctgtggtgct gattttggat ttgaccccta
     3361 tatcagtgat gtaccagatg ttcaatgtga cctgaatacg acaaatgact ttaccgttca
     3421 ttttacggca atgctcaatc cggatgagcg ggtgatcata gcaaagcgcc cgcttaaaaa
     3481 atgtgactcc tggattgagg atgtttatat ctttcaggtg tttaaagaag catggaaatt
     3541 ccataccgat aacagcttac gcggttttcg ggataagcag gctgaactga aactctacgc
     3601 gcgtcattac tcagtggaaa attgcacaga ggaaagctgt cgggattgta attattgcat
     3661 tcggcccagc ttttccctct ctcgtagttc cattattcgc ctgaatgcgg cgaatgctcg
     3721 ttttgtttat caaccgttta cacgcgacca gcggaagagg gggtaattat gcaacccagc
     3781 tccgccatgc aggcattaat tgagcaggtt tatcatattt ttcgtcgtta tccggtgccg
     3841 cagcaatttg tggtttgttg tgaatattgc ctgagtcaac aggaacaaaa agcattacgg
     3901 aatacatcgt tgcgagcgat tccttattcc ttaatcaatg cctggaatag tagtccagga
     3961 cctgatccgc aaaacagcga tgaggttcgt tattttcttc ctcgtttact ggagtttgtt
     4021 gcgcaggggc aatttgacaa tattcacgaa gtattttctc tacggcgtat caaccttgcc
     4081 agtaaagaaa actggcgtga ggatgaacgg gcaatattgc agcgatttgc ctgccagtat
     4141 atggctgatt gggtatctgg cgatgaggct gtcgaattac agtatatgct ggaaatgttt
     4201 tcccgtgccg atatcgctct ttcgccattg cttgatgcca tcatctctgt acccggttac
     4261 caacccacag tatcgatggc atgtttgctg tggcataaca gggaagagaa tattcagaat
     4321 agtcgttttg agcaggatga tgacgataaa gccattaccg cccaaatcaa tgcctgggcc
     4381 ttcaacaatc aattcatcct gaaagaacgt gcgcggcagg cgattgagaa cctgcttaat
     4441 cagcccgaac agggcacaca ttatcaggtc tgggaagacg actggatgat tgatgagtgt
     4501 ttatgcgcga tgtatgacgc atcatccgaa tcaccaggaa aataaaaatg attaaattcc
     4561 ggctttatat tccccctgta attctcggtt ttgttatcgt gccattattg gtatggccga
     4621 cggttattgc cttagccgta cttatattca cgttaacttt tctggctgaa ataatattct
     4681 cctttccgct cctggttgtg cgtatttctc ttcaggaatt acaacttgag ttaatggttg
     4741 aatatgcact ttttttcagt gtaatgggtg gcatcggttg gcaattctcc cgcagaacgc
     4801 ctcctgaatt aaaaaacaga ctgcattact ggctggtctt ttctccggcc tatttctggt
     4861 taattctctc gaatttcatt ctttatatta ctccagagaa atcagcgttg ctggaaaata
     4921 tccgaaactt ctttctgacg tttgtctggc ttcccctgaa tttttcccct ttttggccgc
     4981 agccgtggac tgattttgtc ggcccgatta gtgcacagct tggttttgcg ttgggatatt
     5041 actgccagtg gcgtagcaaa aatagaagcc aggggcagaa gtgggttgag tggggaacgt
     5101 gcttaagttt ggcgatttta gctcaggggc cgttattcaa ttatttacaa tgaatagtct
     5161 aaggaatcca aatagcccgg ttgcacttca caaccgggca tcatccttac gacgccagcg
     5221 acaacttctg ctgcgtatac tccaccagcc actgactcac acgagattgt tgttccgcat
     5281 tcagccacat gccttgtttt gtgcgacgcc acagggcatc gtcggcgcgg cggacccatt
     5341 cgtgatccac caggtatttc agctccgctt cgtagaactc atgaccgaaa tcttccccga
     5401 gatcgcttac cgtccccgca ttgccgagca gcaactcgct gttgctgccg tacgtgcgcg
     5461 cgtaatggcg tgccagcgat tcagtcagga acggatagcg gcggcgcagg cgagcggcat
     5521 aatcgtcgcg gtcgccttca atggcaccac ccggcagcac gctctctttc gtccacgccg
     5581 gaccaatgcc ctgataatat ggcgtcagtt tttccagcgc gtgctccgcc agtttgcggt
     5641 aggtggtcag cttaccgccg aataccgaca gcagcggcgc tttgccgttt tcatcatgga
     5701 tatcaagggt gtaatcacga gtgatagcct gcggcgaatc ggactcatca tcacacagcg
     5761 gacgcacgcc ggagtaagtc cagacgatat cgtcccggct taactgcttt ttaaagtgcg
     5821 tgttatacac tttcagcagg taattgattt cactctcttc aatcttcact gcttttggat
     5881 cacctttgta ttccacatcg gtggtgccga tgatggaaaa ctcgtccatc cacgggatca
     5941 cgaagacaat acgtttatct tcgttttgca gaatgtaggc ctgcttttgt gtatgaacgc
     6001 gcggcacgac aatatggctg cctttgatca ggcgaatgcc ataaggcgaa ggcagatgca
     6061 tcccgtcgtc gaagaactgc tttacccacg ggccggtggc gttcacgagg ccgcgcgctt
     6121 gccagctata ttttttgccg gtatcgatat cttccgcttc cacaatccac aggccgtttt
     6181 cgcggcgagc agaagtggca cgagtacgag taagcacttc accgccttta cgcactacca
     6241 tctgggcgtt ggcgagtacc agacgggcgt cgtctaccca acagtcggaa tattcgaatc
     6301 cgcgcttaat ttccggtttt aacactgaat ttgcgccaaa acgcaaaccg gttgatcccg
     6361 gcaggctggt gcgtttaccc agatgatcgt acataaacag accaatgcga atcatccacg
     6421 ccgggcgcag atgcggacga tgcggcaggc gaaaacgcat cgggaaggcg atatgcgggg
     6481 ccattttcag tagcacttca cgttcagcca gcgcctcgct gaccaggcgg aattcatagt
     6541 gctcaaggta gcgcaggcca ccgtgaatga gttttgaact ggcggaagag gtcgcgcaag
     6601 cgagatcctg cgcctccagc atcagcacgg ataaaccgcg tccagcggcg tctgccgcga
     6661 taccagcacc attgatgccg ccacctatca caatcagatc tttggtttcc atgctgccct
     6721 cattcacttt cgttaaagct cataaatgtt cgttatcgaa catattagca aagaatcgcg
     6781 ctttaggtaa cattgaaaaa acattttaga gtgatatgta taacattatg gcgtttatct
     6841 gccgcttcga cgtaaactgt gcggtaaatt tgcccacttg tttgtaaaga aagagagacg
     6901 catggagcag ttcgaatgta ttaacgttgc cgtcgcgcac cagaagttgc aggaaaaaga
     6961 ggcggtgctg gtcgatattc gcgatccaca gagtttcgca atggggcatg cggtgcaggc
     7021 tttccattta actaacgaca cactgggcgc gtttatgcgt gataacgact ttgacacacc
     7081 ggtgatggtg atgtgttatc acggcaatag cagcaaaggc gcggcgcagt atctgctgca
     7141 acagggctac gatgtggtct atagcattga cggcggcttt gaagcctggc ggcgtcagtt
     7201 tcctgcagag gtggcgtacg gcgcgtaacg ctttatactg tccccttttg tgtggaataa
     7261 gcgacagcaa cgatgttgat gattacctct tttgctaacc cccgcgtggc gcaggcgttt
     7321 gttgattaca tggcgacgca gggtgttatc ctcacgattc aacaacataa ccaaagcgat
     7381 gtctggctgg cggatgagtc ccaggccgag cgcgtgcggg cagagctggc gcgttttctc
     7441 gaaaacccgg cagatccgcg ttatctggcg gccagctggc aggcgggcca taccggcagt
     7501 ggcctgcatt atcgccgtta tcctttcttt gctgccttgc gtgaacgcgc aggtccggta
     7561 acctgggtga tgatgatcgc ctgcgtggtg gtgtttattg ccatgcaaat tctcggcgat
     7621 caggaagtga tgttatggct ggcctggcca ttcgatccaa cactgaaatt tgagttctgg
     7681 cgttacttca cccacgcgtt aatgcacttc tcgctgatgc atatcctctt taacctgctc
     7741 tggtggtggt atctcggcgg tgcggtggaa aaacgcctcg gtagcggtaa gctaattgtc
     7801 attactctca ttagcgccct gttaagcggc tatgtgcagc aaaaattcag cgggccgtgg
     7861 tttggcgggc tttctggcgt ggtgtatgcg ctgatgggct acgtctggct acgtggcgaa
     7921 cgcgatccgc aaagtggcat ttacctgcaa cgtgggttaa ttatctttgc gctgatctgg
     7981 attgtcgccg gatggtttga tttgtttggg atgtcgatgg cgaacggagc acacatcgcc
     8041 gggttagccg tgggtttagc gatggctttt gttgattcgc tcaatgcgcg aaaacgaaaa
     8101 taattccagg gatttataaa tgaaacaaac acaacgtcac aacggtatta tcgaactggt
     8161 taaacagcag ggttatgtca gtaccgagga actggtggag cacttctccg tcagcccgca
     8221 gactattcgt cgcgacctca acgagctggc agagcaaaac ctgatcctgc gccatcatgg
     8281 cggtgcggcg ctgccttcca gttcggttaa cacgccatgg cacgatcgta aagccaccca
     8341 gaccgaagaa aaagagcgca tcgcccgcaa agtggcggag caaattccta atggctcaac
     8401 gctgtttatc gatatcggca ccaccccgga agcggtggcg cacgcgctgc tcaatcacag
     8461 caatttgcgc attgtcacca acaatcttaa cgtcgctaac acgctgatgg taaaagagga
     8521 ttttcgcatt attctcgccg gtggcgaatt acgtagccgc gatggtggga tcataggcga
     8581 agcgacgctc gattttatct cccagttccg ccttgatttc ggcattctgg ggatcagcgg
     8641 catcgatagc gacggctcgt tgctggaatt cgattaccac gaagttcgca ccaaacgcgc
     8701 cattattgag aactcgcgcc acgttatgct ggttgtcgat cactcgaaat ttggccgtaa
     8761 tgcgatggtc aatatgggga gcatcagcat ggttgatgct gtctacaccg acgccccgcc
     8821 gccagtaagc gtcatgcagg tgctgacgga ccatcatatt caactggagc tgtgctgatc
     8881 ctgcacggct tcccacgtca gaccaaaacg cgccaggtat ttgcgtagcc gatctgcgtc
     8941 gttgacgctg gctttgccct ggcgcgaaac atcaaaaagc tggcgaccgg cagcagaaag
     9001 tgactttgcc tggcggcaga tggctattac gtgttccagt tgcatacggt cgaagagatc
     9061 gatgttttct gcctctgcgc ccagcaacgc cgtaatcgcg gaggggcgac tctcctgcca
     9121 gttatagcgc agacggttta tctcatcttc caccacttcc cgagtgatgc gtccgctagt
     9181 ggcaaaggtt gccatccgcg tgacgctggc agaaagttcg cgaaagttac cgcgccatgt
     9241 tgcctgggga gaggtcgcaa aagccagcca ggcgcgccgc gcttcggtgt taaaacgcac
     9301 gctgtcgcca gtgagtgagg cgtggcgctc cacttcataa tcaaggttcg gttcaatatc
     9361 ttcctggcgc tggctcagcc ccggcaaggt gaaggtccag agattgatcc gcgcgtacag
     9421 atcttcgcgg aatttgcctt cggcaaccag ctgacgcaaa tcgcgcaccg ttccggcgat
     9481 aagctgaaag tcgctactca cctggcgatc gctgccaaac ggataaaagg ttttctcttc
     9541 aatggctttc agcagcattg cctgttcgtc tgcgcccagt tcgccaatct catcaagaaa
     9601 taacatcccg ccgttggcgc tgcgtaataa tccttcccgc gactcccgcg ccccggtaaa
     9661 cgcgcctttc acatgaccaa atagcgtcga catggcggta tcgccacgca gagtggcgca
     9721 gttcatttct acaaatgcgc cgctaaactg atgccgtgcc tgttttaatt cgaagatgcg
     9781 tcgtgccaga aatgacttgc ccgcacctgt cgggccgtta agcaggatcg gtgcgcggga
     9841 tttaatcgcc actttttcaa tctgctcaat catgcggttg aagtgggggt tacgcgtggc
     9901 aatgccggac ttaagaaaat cgagcgtttg ctgccgttcc tcggcaaagc ggctggcgat
     9961 ggcgttataa cggcttaaat cgagatcgat aatcgtcact tcacctgcgc cgcgcggctg
    10021 ttcttttttg cgcggtgggg aagattgtat cagccgggcg ggcaggtaac gcgcttccgc
    10081 cagcagaaac cagcaaatct gtgcgacgtg ggtgccagtg gtgatgtgaa ttaaatagtc
    10141 ttctttatca ggttgaaacg cgtagccacg ggcgaaatca tgcaggcagg cgtaaacctc
    10201 ttcgaaatcc cacgggttat gcagttcaat ctcaacgctc accacttcag tttctggcga
    10261 aacgctggcg atatcgcatt taagcgtttc aaacagcgag cgcgagcggg cgtcgtgcaa
    10321 caattccagt cgattgatga ctaacgattc ttgctggcat agacagagtg acggacgcca
    10381 tttcgaccag cgctgactgc cgcgcccggc ataatccagc accgttccga caaagccaaa
    10441 agccactgtt ttacgcatc
//
LOCUS       DAZKGF010000172        10272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZKGF010000172 DAZKGF010000000
VERSION     DAZKGF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.172"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            160..1554
                     /gene="galP"
                     /locus_tag="ACHOQV_004151"
     CDS             160..1554
                     /gene="galP"
                     /locus_tag="ACHOQV_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFP9632437.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1631..2128
                     /locus_tag="ACHOQV_004152"
     CDS             1631..2128
                     /locus_tag="ACHOQV_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFP9632438.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2223..2930
                     /gene="endA"
                     /locus_tag="ACHOQV_004153"
     CDS             2223..2930
                     /gene="endA"
                     /locus_tag="ACHOQV_004153"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFP9632439.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            3010..3741
                     /gene="rsmE"
                     /locus_tag="ACHOQV_004154"
     CDS             3010..3741
                     /gene="rsmE"
                     /locus_tag="ACHOQV_004154"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP9632440.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3754..4704
                     /gene="gshB"
                     /locus_tag="ACHOQV_004155"
     CDS             3754..4704
                     /gene="gshB"
                     /locus_tag="ACHOQV_004155"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFP9632441.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4813..5376
                     /locus_tag="ACHOQV_004156"
     CDS             4813..5376
                     /locus_tag="ACHOQV_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFP9632442.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5376..5792
                     /gene="ruvX"
                     /locus_tag="ACHOQV_004157"
     CDS             5376..5792
                     /gene="ruvX"
                     /locus_tag="ACHOQV_004157"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP9632443.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5966..6946)
                     /locus_tag="ACHOQV_004158"
     CDS             complement(5966..6946)
                     /locus_tag="ACHOQV_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFP9632444.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRTILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            6964..7668
                     /gene="yggS"
                     /locus_tag="ACHOQV_004159"
     CDS             6964..7668
                     /gene="yggS"
                     /locus_tag="ACHOQV_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFP9632445.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLDVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7686..8252
                     /gene="yggT"
                     /locus_tag="ACHOQV_004160"
     CDS             7686..8252
                     /gene="yggT"
                     /locus_tag="ACHOQV_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFP9632446.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8249..8539
                     /gene="yggU"
                     /locus_tag="ACHOQV_004161"
     CDS             8249..8539
                     /gene="yggU"
                     /locus_tag="ACHOQV_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFP9632447.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8547..9140
                     /locus_tag="ACHOQV_004162"
     CDS             8547..9140
                     /locus_tag="ACHOQV_004162"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFP9632448.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLIYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9133..10269
                     /gene="hemW"
                     /locus_tag="ACHOQV_004163"
     CDS             9133..10269
                     /gene="hemW"
                     /locus_tag="ACHOQV_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP9632449.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2414 a         2699 c         2709 g         2450 t
ORIGIN      
        1 atctatgatg atataactca attattttca tgcacttaaa tcataaccaa gataaatgtt
       61 agtgtaagcg attacactga tgtgatttgc ttcacatctt tttacgtcgt actcacctat
      121 cttaattcac aataaaaaat aaccatattg gagggcatca tgcctgacgc taaaaaacag
      181 gggcggtcaa acaaggcaat gacgtttttc gtctgcttcc ttgccgctct ggcgggatta
      241 ctctttggcc tggatatcgg tgtaattgct ggcgcactgc cgtttattgc agatgaattc
      301 cagattactt cgcacacgca agaatgggtc gtaagctcca tgatgttcgg tgcggcagtc
      361 ggtgcggtgg gcagtggctg gctctccttt aaactcgggc gcaaaaagag cctgatgatc
      421 ggcgcaattc tgtttgttgc cggttcactg ttctctgcgg ctgcgccaaa cgttgaagta
      481 ctgattcttt cccgcgttct gctggggttg gcggtgggtg tggcttctta taccgcaccg
      541 ctgtacctct ctgaaattgc gccggaaaaa attcgcggca gcatgatctc gatgtatcag
      601 ttgatgatca ctatcgggat cctcggtgct tatctttctg ataccgcctt cagctacacc
      661 ggtgcatggc gctggatgct gggtgtgatt atcatcccgg caattttgct gctgattggt
      721 gtcttcttcc tgccagacag tcctcgttgg tttgccgcca aacgccgttt tgttgatgcc
      781 gaacgcgtgc tgctacgcct gcgtgacacc agcgcggaag cgaaacgcga actggatgaa
      841 atccgtgaaa gtttgcaggt taaacagagt ggctgggcgc tgtttaaaga gaacagcaac
      901 ttccgccgcg cggtgttcct tggcgtactg ttgcaggtaa tgcagcaatt caccgggatg
      961 aacgtcatca tgtattacgc gccgaaaatc ttcgaactgg cgggttatac caacaccacc
     1021 gagcaaatgt gggggacggt gatcgtcggc ctgaccaacg tactcgccac ctttatcgca
     1081 atcggccttg ttgaccgctg gggacgtaaa ccaacgctaa cgctgggctt cctggtgatg
     1141 gctgctggca tgggcgtact cggtacaatg atgcatatcg gtatccactc tccgtcggcg
     1201 cagtatttcg ccatcgccat gctgctgatg tttattgtcg gttttgccat gagtgccggt
     1261 ccgctgattt gggtactgtg ctccgaaatt cagccgctga aaggccgcga ttttggcatc
     1321 acctgctcca ccgccaccaa ctggattgcc aacatgatcg ttggcgcaac gttcctgacc
     1381 atgctcaaca cgctgggtaa cgccaacacc ttctgggtgt atgcggctct gaacgtactg
     1441 tttatcttgc tgacattgtg gctggtaccg gaaaccaaac acgtttcgct ggaacatatt
     1501 gaacgtaatc tgatgaaagg tcgtaaactg cgcgaaatcg gcgctcacga ttaatctcct
     1561 caggcttcct cccgtcgcgg aggaagccac ctcttgcagt catcttttct tcgctctatc
     1621 ctctgccgct atgaaaacat cccgtctccc tatcgccatc cagcaggccg ttatgcgccg
     1681 cctgcgggaa aaactcgccc aggccaacct gaagctaggg cgtaactacc cggagccaaa
     1741 actctcttac acccagcgcg gaacctccgc cggaacagcc tggctggaaa gctatgaaat
     1801 ccgcctcaat cccgttttgc tgttggaaaa cagtgaagct tttattgaag aagtggtacc
     1861 gcacgaactg gcacatttgc tggtatggaa acatttcggc cgcgtagcgc cacatggcaa
     1921 agagtggaag tggatgatgg aaagcgtgct gggcgttccc gcccgtcgta cgcatcagtt
     1981 cgaactgcaa tccgtgcgtc gcaacacctt cccctaccgc tgcaagtgcc aggagcatca
     2041 gcttaccgta cgccgccata atcgcgtagt tcgtggcgag gccgtctatc gctgtgttca
     2101 ctgcggtgaa cagctggttg cgaaataacc acctgaacta tcaggaactt tcctgatctg
     2161 gctgattgca taccaaaaca actttcgcta cgttgctggc tcgttttaac acggagtaag
     2221 tgatgtaccg ttatttgtct attgctgcgg tggtactgag cgcagcattt tccggcccgg
     2281 cgttggccga aggtatcaat agtttttctc aggcgaaagc cgcggcggta aaagtccacg
     2341 ctgatgcgcc cggtacgttt tattgcggat gtaaaattaa ctggcagggc aaaaaaggcg
     2401 ttgttgatct gcaatcgtgc ggctatcagg tgcgcaaaaa tgaaaaccgc gccagccgcg
     2461 tagagtggga acacgtcgtt cccgcctggc aattcggtca ccagcgccag tgctggcagg
     2521 acggtggacg taaaaactgc gccaaagatc cggtctatcg caagatggaa agcgatatgc
     2581 ataacctgca gccgtcagtc ggtgaggtga atggcgatcg cggcaacttt atgtacagcc
     2641 agtggaatgg cggcgaaggc cagtacggtc aatgcgccat gaaggtcgat ttcaaagaaa
     2701 aagccgccga accaccagcg cgtgcacgcg gtgccattgc gcgcacctac ttctatatgc
     2761 gtgaccaata caacctgaca ctctctcgcc agcaaacgca actgttcaac gcatgggaca
     2821 agatgtatcc ggttaccgac tgggagtgcg agcgcgatga acgcatcgcg aaggtgcagg
     2881 gcaatcataa cccgtatgtg caacgcgctt gccaggcgcg aaagagctaa cctacactag
     2941 cgggattctt tttgttaacc cctaccccac gcgtacaacc gcgtggggag acgacgcgga
     3001 tttttaacta tgcgtatccc ccgcatttat catcctgaac cactgaccag ccattctcac
     3061 atcgcgcttt gcgaagatgc cgccaaccat atcgggcgcg tactgcgtat ggggccgggg
     3121 caggcgttgc aattgtttga cggtagcaac caggtctttg acgccgaaat taccagcgcc
     3181 agcaaaaaaa gcgtggaagt gaaggtactg gaaggccaga tcgacgatcg cgaatctccg
     3241 ctgcatattc acctcggtca ggtgatgtcg cgtggtgaaa aaatggaatt tacgatccag
     3301 aaatcgatcg aactcggtgt aagcctcatt acgccacttt tttctgagcg ctgcggcgtt
     3361 aaactggata gtgaacgtct gaacaagaag ctccagcagt ggcagaagat tgcaattgct
     3421 gcctgtgagc agtgtggtcg taaccgggtg ccggaaatcc gtccagcgat ggatctggaa
     3481 gcctggtgtg cagagcagga tgaaggactg aaactgaatc ttcacccgcg cgccagtaac
     3541 agcatcaata cgttgccgtt accggttgaa cgtgtccgcc tgctgattgg cccggaaggc
     3601 ggtttatcgg cagatgaaat tgccatgact gcccgctatc aatttactga tatcctgttg
     3661 ggacctcgcg ttttgcgtac agagacaact gcgctcaccg ccattaccgc gctacaagta
     3721 cgatttggcg atttgggcta acggagaaga ataatgatca agctcggcat cgtgatggac
     3781 cccatcgcaa acatcaacat caagaaagat tccagtttcg ctatgttgct ggaagcacag
     3841 cgtcgtggtt acgaacttca ctatatggag atgggcgatc tgtatctgat caatggcgaa
     3901 gcccgcgccc atacccgcac gctgaacgtg aagcagaact acgaagagtg gttttcattc
     3961 gtcggtgaac aggatctgcc gctggccgat ctcgatgtga tcctgatgcg taaagacccg
     4021 ccgtttgata ccgagtttat ctacgcgacc tatattctgg aacgtgccga agagaaaggg
     4081 acgctgatcg ttaacaagcc gcagagcctg cgcgactgta acgagaaact gtttaccgcc
     4141 tggttctctg acttaacgcc agaaacgctg gttacgcgca ataaagcgca gctaaaagcg
     4201 ttctgggaga aacacagcga catcattctt aagccgctgg acggtatggg cggcgcgtcg
     4261 attttccgcg tgaaagaagg cgatccaaac ctcggcgtga ttgccgaaac cctgactgag
     4321 catggcactc gctactgcat ggcgcaaaat tacctgccag ccattaaaga tggcgacaaa
     4381 cgcgtgctgg tggtggatgg cgagccggta ccgtactgcc tggcgcgtat tccgcagggg
     4441 ggcgaaaccc gtggcaatct ggctgccggt ggtcgcggtg aacctcgtcc gctgacggaa
     4501 agtgactgga aaatcgcccg tcagatcggg ccgacgctga aagaaaaagg gctgattttt
     4561 gttggtctgg atatcattgg cgaccgtctg actgaaatta acgtcaccag cccaacctgt
     4621 attcgtgaga ttgaagcaga gtttccggtg tcgatcaccg gaatgttaat ggatgccatc
     4681 gaagcacgtt tacagcagca gtaacccacc ttagcgagaa ggatctcgtt gagactctga
     4741 gtgacagcgc ccttctttcc acgcatactg ggcgctgttg cttttttgaa ccaggaaaca
     4801 gaacctctga caatgaattt acagcatcac tttcttattg ccatgcctgc tctccaggat
     4861 ccgattttcc gtcgttccgt ggtctacatt tgcgaacata ataccaatgg tgcaatgggg
     4921 atcatcgtca acaagccgct ggaaaatctc aaaattgaag ggattctgga aaagctgaag
     4981 atcacgccgg agccgcgtga tgaatcaatc cgtctggata aaccggttat gctcggtggt
     5041 ccgctggctg aagatcgcgg gtttattttg catactccgc cctctaattt tgcttccagc
     5101 attcgcattt cagacaacac ggtgatgacc acttcccgcg atgtgctgga aacgctcggc
     5161 accgataaac aaccgtctga cgtattggtg gctctgggct atgcctcctg ggagaaaggt
     5221 caactggaac aagaaattct cgataacgcg tggctaacgg ccccggcgga tctgaatatt
     5281 ctgttcaaaa caccgattgc cgaccgctgg cgcgaggcgg caaaactgat tggtgtggat
     5341 attctcacca tgcctggtgt ggcagggcac gcctgatgag tggaacctta ctcgccttcg
     5401 acttcggtac caaaagcatt ggtgtagcgg tcggccaacg cattaccggc accgctcgcc
     5461 cattgcctgc aattaaagca caggacggta cgccggactg gaacattatc gagcgtttac
     5521 tgaaagagtg gcagcctgac gaaatcatcg tcggtttgcc gctgaatatg gacggcaccg
     5581 aacagccgtt aaccgccaga gcgcgtaaat ttgccaaccg tattcatggt cgtttcggtg
     5641 ttgaagtaaa gctccatgac gagcgtctta gcaccgtgga ggcacgttcc ggtctgtttg
     5701 aacagggcgg ctatcgggcg ctcaacaaag gcaaagtcga ctctgcctct gcggttatta
     5761 ttctcgaaag ctatttcgag cagggatatt aaggcgattt actagcgcac agatttctga
     5821 caatgtgctc gttgtttcat gttggatgcg gcgtaaacgc cttatccaac ctacagtgag
     5881 tgcgaattca aagttacgct tcccttgtag gcccgataag cttgcgcatc gggcatattt
     5941 acaattcgta tcagacacag caacgtcaca agcgcccttc tttcacccgc tgctgataac
     6001 tctgctgaaa cgttatcatc cccacctgct gcccggtttg aataacatgc ggtaactggt
     6061 gggttttccc ttcacgaatc aaattcccca ccgcgggtgt gttaatcagc aattcaaaca
     6121 acgccacgcg tccttcctgt ttatccactt ccagcttttg tgacaacacc gcccgtaaac
     6181 tacctgccag ttgattacgc acgggatctt tttcctgcgc cggaaatgaa tctaccagtc
     6241 gctcaactgc ctgcgccgca ccacgcgtat gtaatgttgc cagcaccaaa tgcccggttt
     6301 ctgccgccgt cagcgccaga cggattgtct cgctgtcacg cagctctccg agcaaaatca
     6361 catcaggatc ttcccgcaat gcagcccgca atcccgatgc gaacgtcata caatgcaaac
     6421 cgatttcccg ctgctggatc aaacatcgct ggcttgcata gagatattcg acaggatctt
     6481 ccagcgtcag aatatgcgca tcggcatgtt gattgagata gccaaccatc gccgccagcg
     6541 tggtagattt gccgctcccc gtcgcccccg tcaccagaat caggccattc tcgctcttga
     6601 gtaattccgg caataccggt ggcgcaccaa gctgttcgag ctgtggacag tgcgaaggta
     6661 acaaccgtaa tgccagagaa ataccttgcc gttgcgcgaa cgcactgcca cgcaaccgct
     6721 ggttttccgc cagcgacaca gcaaaatcca actgaccatt ctccagcaat attgtccgct
     6781 gatcgtcatc cagccactcc cgcagtagct cttcgacgtc cggcgcatca aacggcgcag
     6841 cttccattcg cccgcgaatg cgccatcgtg cgggccaggc gctgcacagg tgtagatccg
     6901 agacgttatg ctttacacta agggccacaa tttcttccat attcatacta agatcctcgg
     6961 aaaatgaacg atattgcgca taacctggca caggtccggg acaaaatctc agcggctgca
     7021 acgcgttgcg gccgttctcc agaagaaatt acgctgcttg cagtcagtaa aacaaaacct
     7081 gcgagcgcca tcgcagaagc cattgatgcc gggcagcgtc aatttggtga aaactacgtt
     7141 caggaagggg tagataaaat tcgccacttt caggaactgg gcgtaacagg attagaatgg
     7201 cattttattg gcccgttgca gtctaataaa agccgcctgg tggcagagca tttcgactgg
     7261 tgtcatacca tcgaccgttt gcgcattgct acccgcctca acgatcagcg cccggcagaa
     7321 cttccccctc ttgacgtact gattcaaatt aatattagtg atgaaaacag taagtccggg
     7381 attcaactgg cagaactgga cgagctggca gctgcggtcg ctgaactacc gcgtttacgt
     7441 ctgcgcgggc tgatggcaat ccctgcgcct gagtcagaat atgtaaggca gtttgaagtt
     7501 gcacgccaaa tggctgtagc atttgccgga ctgaaaacgc gctacccgca tatcgacacg
     7561 ctctctctgg gaatgtcgga cgatatggaa gccgccattg cggcaggtag cacgatggtt
     7621 cgtatcggca ctgcaatttt tggtgcgcgt gattactcta aaaaataagg aattaaagga
     7681 acgccatgaa tacgttgact ttcctgcttt caacggtcat tgagctgtat accatggtgc
     7741 tgttattacg catctggatg cagtgggctc attgtgattt ttacaacccc ttctcacagt
     7801 ttgtagtgaa ggtaacgcag ccaattatcg ggccactgcg ccgcgttatt ccggcaatgg
     7861 gacccattga cagcgcctcg ctgctggttg cctatattct cagttttatc aaagccatcg
     7921 tgctgtttaa agtggtgacc ttcctgccaa tcatctggat tgccggttta ctgattctgc
     7981 tgaaaaccat cggcctgctg attttctggg tcctgctggt gatggcgatt atgagctggg
     8041 taagccaggg tcgtagtccg attgaatacg tgctgattca gcttgccgat ccgctgctgc
     8101 gcccgattcg ccgcctgcta ccggcaatgg gtgggattga tttctcgccg atgatcctcg
     8161 ttctgctgct gtatgtcatc aatatgggtg tcgcagaagt attacaggca accggaaaca
     8221 tgctgctgcc ggggctgtgg atggcgttat gagtgccgta acagttaatg atgacggtct
     8281 ggttttacgg ctctatattc agccgaaagc cagccgtgat tctattgtcg gtttacatgg
     8341 cgacgaggta aaagtcgcca ttaccgcgcc gccggttgac ggccaggcca acagccatct
     8401 ggtgaagttt ctcggtaagc aattccgggt tgccaaaagc caggtggtga ttgaaaaagg
     8461 cgaacttggc cgccacaaac aaattaaaat cattaatccg caacaaatcc cgccagaaat
     8521 cgcggcgtta attaattagg tatcctatgc aaaaagttgt cctcgcaacc ggcaatgccg
     8581 gtaaagtgcg tgagctggcg tcgctgctta gcgacttcgg tcttgatatc gtggcccaaa
     8641 cagacctcgg cgtagattcc gctgaagaaa ccggcctgac ctttatcgaa aacgcgattc
     8701 tgaaagcgcg ccatgcggca aaagtgaccg gtttaccggc aattgccgac gactccggtc
     8761 tggcggtaga tgcgctgggt ggcgcgccgg gtatttactc cgcgcgttat tccggtgaag
     8821 acgcgaccga tcaaaagaac ctgcaaaaac tgctggaaac aatgaaagac gtaccggacg
     8881 accaacgtca ggcgcgtttc cactgcgtgc tgatatatct gcgtcacgcg gaagatccga
     8941 ctccgctggt gtgccacggt agctggccgg gcgtgattac tcgtgaacca gcgggtactg
     9001 gtggctttgg ttatgatcca attttcttcg ttccttctga agggaaaacc gctgccgaac
     9061 tgacccgcga agaaaagagc gccatttccc accgtggtca ggcattgaaa ctgctgctgg
     9121 acgctttacg taatggttaa attaccgccg ctgagtctct acattcacat cccgtggtgc
     9181 gtgcagaaat gcccgtactg cgatttcaac tctcacgcgt tgaaaggaga agtgccgcac
     9241 gacgattatg ttcagcatct gcttaacgat ctggacaacg atgtggctta cgctcagggc
     9301 cgtgaagtgc agacaatctt tattggcggt ggtacgccga gcctgctttc cggcccggcg
     9361 atgcaaacgc tgctggacgg cgtgcgtgcg cgtttgccgc tggcagcgga tgcagaaatt
     9421 acgatggaag cgaacccagg cacggtagaa gcggatcgct ttgtcgatta tcagcgtgct
     9481 ggtgtgaacc gcatctctat tggtgtgcag agttttagcg aagaaaagct gaaacgactc
     9541 gggcgtattc atggcccgca agaagcgaaa cgagcggcaa agctggcgag cggtttaggg
     9601 ttacgtagtt ttaaccttga tttgatgcat gggctgccgg atcaatcact ggaagaggcg
     9661 ctaggcgatc tgcgccaggc tattgaactg aatccgccac atctttcctg gtatcaactg
     9721 accatcgaac ctaatacgct gtttggctcg cgccctcctg tactgccgga cgatgacgcg
     9781 ctgtgggata ttttcgaaca ggggcatcag ttattaaccg cagcgggcta tcagcagtat
     9841 gaaacatccg cttacgccaa acccggttat cagtgccagc acaatctcaa ctactggcga
     9901 tttggcgact acatcggtat tggctgcggc gcgcacggca aagtgacctt cccggatggg
     9961 cgcattctgc gtaccaccaa aacgcgtcat ccgcgtggtt ttatgcaggg aagatatctg
    10021 gaaagccagc gtgatgtcga agctgcagat aaaccgtttg agttctttat gaatcgcttc
    10081 cgtctgcttg aggccgcgcc gcgcgtggag tttagtcagt atactggcct ttctgaagaa
    10141 gttattcgct ctcagttaga cgaggctatc gctcagggtt atctcacaga atgtgcggat
    10201 tactggcaga taaccgagca tggtaaatta ttcttaaact cacttcttga attattcctc
    10261 gctgagtaat ca
//
LOCUS       DAZKGF010000173        10195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZKGF010000173 DAZKGF010000000
VERSION     DAZKGF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.173"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..738)
                     /locus_tag="ACHOQV_004164"
     CDS             complement(<1..738)
                     /locus_tag="ACHOQV_004164"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9632450.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDAYGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAG"
     gene            complement(760..2061)
                     /gene="ybbY"
                     /locus_tag="ACHOQV_004165"
     CDS             complement(760..2061)
                     /gene="ybbY"
                     /locus_tag="ACHOQV_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFP9632451.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTSDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(2118..3479)
                     /gene="allB"
                     /locus_tag="ACHOQV_004166"
     CDS             complement(2118..3479)
                     /gene="allB"
                     /locus_tag="ACHOQV_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFP9632452.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDELGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(3540..4994)
                     /gene="ybbW"
                     /locus_tag="ACHOQV_004167"
     CDS             complement(3540..4994)
                     /gene="ybbW"
                     /locus_tag="ACHOQV_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFP9632453.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLVICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFMVMRGQINLDELYTAAGDFKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTAAEKTGEQKVTG"
     gene            complement(5164..6042)
                     /gene="glxR"
                     /locus_tag="ACHOQV_004168"
     CDS             complement(5164..6042)
                     /gene="glxR"
                     /locus_tag="ACHOQV_004168"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP9632454.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(6142..6918)
                     /gene="hyi"
                     /locus_tag="ACHOQV_004169"
     CDS             complement(6142..6918)
                     /gene="hyi"
                     /locus_tag="ACHOQV_004169"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFP9632455.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHFTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(6931..8712)
                     /gene="gcl"
                     /locus_tag="ACHOQV_004170"
     CDS             complement(6931..8712)
                     /gene="gcl"
                     /locus_tag="ACHOQV_004170"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFP9632456.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMSVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTEACFMHYE"
     gene            complement(8802..9617)
                     /gene="allR"
                     /locus_tag="ACHOQV_004171"
     CDS             complement(8802..9617)
                     /gene="allR"
                     /locus_tag="ACHOQV_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFP9632457.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(9695..10177)
                     /gene="allA"
                     /locus_tag="ACHOQV_004172"
     CDS             complement(9695..10177)
                     /gene="allA"
                     /locus_tag="ACHOQV_004172"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFP9632458.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
BASE COUNT         2723 a         2785 c         2313 g         2374 t
ORIGIN      
        1 acccgccatc tggcagacgt tgatttcagt ttgctgttgt aaaacacggg cgtaattttg
       61 cagcccctgc tccagctctt cgaccatttc cggcgttgcc cctttttgcg ggccgaacac
      121 cgccgccgcg ccgcgtgccc ctaccagcgg attatcaaca tcacatgcga cttcaatatg
      181 gcagttcgcc agacgcggat cgcattcatc catctcaata cttgccacgc gtgctaaatt
      241 gccgccgtta gccgccagca cctgaccgta ggcatcaagg aaacgcacac cgagcgcctg
      301 cgccatgccc ataccgccgt caaccgtcgc gctaccacca atgccgagaa taatatggcg
      361 aatgccgtta tccagcgcat ggcgaattaa ctcccccgta ccaaaactgg aggccagcaa
      421 cggattacgc ttttcaggcg cgaccagcat caggccactt gctgctgcca tctcaatcac
      481 cgccgttttc ccgtcgccgg taatgccata aaaagcattc actttttcgc ccatcggccc
      541 gcagacttca agcttcacga tgttaccgtc cgtcgcggcg accatcgcct ccaccgtccc
      601 ttcgccgcca tccgctatcg gcaaacagat atagtgcgca tcgggaaaga gggtcgaaaa
      661 cccggcttta attgcctgac aacatttttc tgcacttaag ctctctttaa aagagtctgg
      721 cgcaatgaca atcttcatta tcaatccttt ttcaacaaat tattcgatgc gttcccacgg
      781 aataagatta tccataagaa cagccagtaa aattccgacc agtaagccgt tactgagcag
      841 aggacgaagc gttaatggaa ggtcttgcag atacacaggc ggtaatgcca ttaaaaatat
      901 gccgacaaat aacggcaatg cgagtcgata aatattgcga gcggtaaacg tgatttgctg
      961 gctaaacacc agcgcggaaa agagtaaagg cagataagaa accagcatga ccgcgctact
     1021 cactggtaaa gggatactgc aaaacagtcg cgtgagtgcc ggaaccagtg ccaccagcag
     1081 gcaaataacg ctgccataaa taaacgaacg ccgcgtgtaa tcgctagtct gggttaataa
     1141 accaatggat gaaacaaacg gtgaaaatgg aattactgcc agtggcacgg ttatcagtgt
     1201 cataaatccg gtcgccacaa agctgcgacg ataacgcgta ttccccgcgc cctgctgcgg
     1261 ataaaaaaca tccgtgcccc gaatcgcacc gtaggtattg ctgatattta ccagacctgt
     1321 aatcaccgcc gtcagaataa tccccggcga caaagcacca ccactgccga gcgggaacca
     1381 ctgccagtgc aactcaccgg agagcgaatg cgaagaagga aagcaaaagt accacaacaa
     1441 ccagccggtt atcgtaccga ccagcaggcc gtaacgggca aaacgttgtg gcaggaaaat
     1501 aatcatcgcc agtaccaggc acatcaccgc gaccgagagc gcgaacggcg gtaattgaat
     1561 tttaaaattc gggtcggcta tgccaaacgg cagcccgagc atacctttga aaaagatagt
     1621 ggtcagttgc gcacccagca tcaacataaa caagaccatc accgacggcg taaataaccg
     1681 tgccaggcga tgtcctaatc cgctaaaacc aatcaacatc gtcagcacgc cggagagcgc
     1741 aattcccacc gccagactgg tagcgatatc gttgatcggt gtcccgcgcg atgcttcgcc
     1801 aagggtgata gtcaggatgg ttccccacca caggccgccc ggcccctcca taatagcgcg
     1861 acgatgaccg cagaacgcct gagcgaagca ggccagtgcg gtagcaagaa aagcatattg
     1921 cgtgagcgta agcaggctac tttgcggcaa ctgaaaagca gaaagtagcg taggaggaac
     1981 cacaaccgtg ttgcaaaaaa taaagaaaaa ccactgaaat cctgataaca ggctttcgcg
     2041 gctgactgca aaattgaaca taaaaacacc ttaaccggag aatgcccgcc gcaaggacgg
     2101 gcattgcagg ggccagatta ctgctgatgt ttaaggataa attgaccttt cggcgcaaca
     2161 gggaagccct gttcgatgtc gtaaatcaca tcaccacgta agatggtttt cgtgatacgc
     2221 gcgccaatgg tacggccaac atacgggctg actttgtggc gatattccag atcgtcattg
     2281 gtaagaacat agctgctatt cggctgaatg aagacgaagt cggcatcttt tcctggggcg
     2341 atacggcctt tttgctgcag accgaaaata tctgctgcgt tagtcgccat taatttgccg
     2401 aacatcggca gagacatacc gcgtttctgt accgcttcat cgaacatcac gtccatgcag
     2461 ctttgcagac cggcgatacc accccatgct ttcatgatat tgccggcttt catttccggc
     2521 ggacatggag agtggtcgga aaccaggcag tcgatttcac cgttaaacag tttttcccac
     2581 atgcctttct ggttttccag atcgcggatc ggcggtgaac acttcgccag agtaccgatt
     2641 tcttcgaact gatcggtatc aagtacaaag taatgcgggc aggattcaca agtaacgtcc
     2701 tgaccttcct gacgtgcacg agtcacttct tcaacgcctt ccgggctgct gatatggcaa
     2761 acgtgcagac ggcaaccggc aactttcgcc agatacagca cgcggcgaat cgcttccact
     2821 tcggtaaata ccggacgcga agccacatag tcatgagcag ttacgcgacc ttcacgcttc
     2881 gcttcttcgc ccagttcgtc acagatcagc gcgttttcgc agtgcaccag caccggctgc
     2941 cccagttcgc ccagcttctg cgcacctttg aagaactgcc agtcgtttac atcacggaag
     3001 tcgttgtcga taccgcgatc gccacaggtc gcaacgaagc atttgaagcc gacaacgccc
     3061 acttcatcca gctcatgcag acggtcgatg ttgtaagaca ccaggccacc gagttgtgcc
     3121 gcatcgatag tcagcttgcc tttagcggca tcgaacttca gttcaattga agcgcggtca
     3181 accgttgctg gcagctggtt gagcggcatt tcgatcatgg tggtgatacc accttttgcc
     3241 gctgcgcgag taccagtttc ataaccttcc cagtggctac gacccggctc agaaatatgg
     3301 gtgtgcgcat caaccatgcc tggagaaacc accagaccag acgcatccat cacttctttt
     3361 gcatcgccca gatcctgacc gatagcagca atttttccgc ctttaacggc gatatctaca
     3421 acgcgagctt cgttttctaa aataacggtg ccgtttttaa tgattaaatc aaaagacata
     3481 acaaactcct tgtaaaatac agattggttt tttaactcaa tgttcaatat cgggattaat
     3541 taacctgtga ctttttgttc tcctgttttt tctgctgctg tacgtttttt taataacgcg
     3601 taggcagcga atgcgacaat aacgccgaca aaccatgaaa cgcgtgataa aggctccatg
     3661 aacggaataa atttaccacc gagagataaa ataacggcga ccaaagttac tgagaacgcg
     3721 gtaaggttaa agccgttatc gtaatatttg aaatcacctg ctgcggtata cagttcatca
     3781 agattaattt gtccgcgcat caccataaaa taatgcgcca tcatgacacc aattaccgga
     3841 cccagcattc cgccgataat atcgaggaac agataaatac tgtcctgatt ttccattaat
     3901 ttccacgggc agatcaccag gctgataata ctggcaatca gtacgccgtt tttataggtc
     3961 agtttcgtcg gtgcaatagc ggcaatctga taaccggccg gaataatatt accggtggcg
     4021 ttagtggaaa tggtcgtcat cagaataacc agtaccgcaa agaacgaggc gaacaggctg
     4081 tcccaacgct gaacaatatc cagcacgttc caggtgtcag cgccgtaatg aatactggct
     4141 ccggcaataa tacatacccc ggcgaccgca aacagaatat aggccacaac taaacccagc
     4201 gtttgcccca gcgcctgctc acgaaacgag tgggcgtttt gcgtaaagtc ggatgcgctc
     4261 accgccggtg ccgcccagac cgcaaccacc gcgtttatca ccaccagaaa caggaagcca
     4321 ctgttttctg ctttctgaat accgcccgga atgtagtcaa agattggacc gatcccgacc
     4381 agcgaaatcg cccaaatcgc cataccgccg aaaacgatat agatgcacgg gttaagaatg
     4441 gcagtgaatt tatttaaaac ttttccgccg ccaaaaccaa taccaacgtt aaccagccag
     4501 aagagtaaaa aagtaattaa tcccggtaga gaaaggccta acagcgtgaa atcgccaccg
     4561 agagtcaaaa atcccggcca gattttgcca atcagaatca agcaggccag tgaccccgcg
     4621 taacattgca ggccaaacca cataatggca gcaataccgc ctcttaataa tccgggaaac
     4681 agtgcaccac gtacgccgta agaagcacgc aggatcatgg caaacggtac gccgtattta
     4741 ctgcccgcag caccgtttaa taccattacc gcggcaatga aaaaggcact gaggataatt
     4801 gccagcataa tactaaaggt agacaagccg agaataaaaa agccgccaac catcacataa
     4861 ttgggaacgt tatgaaccga acccatccat aaggtaaaat agttaaatgt tttccaggtc
     4921 cgctgacttt gcgttttcgg cagtagatct tcgctatagc cgcgttgctg gaatagtttt
     4981 ctctgatgtt ccataaatcc ctcttaattt cacgtatcga ataacgttgt tttaatctag
     5041 ctatgaggaa caaataaaat aaaaatatta tttttaatac tgaagcaaca tctttggctg
     5101 ctttcttttt ttaatattgc tggccgattc attttctgat atcggccatt tttattgcgg
     5161 gtatcaggcc agtttatggt tagccattaa ttccagcgcc tgcactaatg cagagtgatc
     5221 caactggctg ccaccgtttg ccgcacaggt attaaacaat tcctggcagg tcgcggtgtt
     5281 tggcaggttc agcgccagtg ctttcgcact ttgcagtgcc aggttgagat ctttctggtg
     5341 cagcgcaatt ttgaaacctg gattaaaggt gcgtttaatc atgcgctcac cgtgaacttc
     5401 cagaatacgt gaggaagcaa agccgcccat cagcgcctgg cgcacacgta ccgggtccgc
     5461 accggctttt gaagcaaaca acaaggcttc agaaaccgct tcaatattga gcgccacgat
     5521 aatctgattt gccactttgc aggtttgacc gtcgccgtta ccgcccacga gagtgatatt
     5581 tttaccgagc agttcaaaca gcggttttac acgttcaaat actgcttcat caccgccaac
     5641 cataatcgat aacgtccctt cacgcgcgcc gatttcaccg ccggagactg gcgcatcgag
     5701 ataatcgccg cccagttcat tcacctgacg agcgaaacgt ttagtttcaa tcggggaaat
     5761 ggagctcata tcaacaatgg ttttgccctt cagcgaggct ttggtacaac cattttcacc
     5821 aaacagaacg tcttcaacct gtggcgtatc cggcaccata ataaaaatga tgtccgatgc
     5881 atctgttacc tggcgcgcag tttcaacact aacggcgccc agtgacagta attcatcagc
     5941 aaccggacca atggttgtga catgcagttg atgaccggca cgcgccagat taatggccat
     6001 cggtgtaccc ataatgccta agccaataaa tcccagtttc ataattaacc tcttttaaat
     6061 ttcgcttttc ctgaattcag acaacacgat cccctgccaa cttagcggcc taaaaaagca
     6121 ttgtctgaat agcgttacgt tttaacggta cggatccatc cagcgtaaac cggcttccgt
     6181 ggtggtttgg ggtttatatt cacagccaac ccaaccgttg tagtcagaat tttcaattac
     6241 cgtaaagaga tattcgtaat taatttctcc ggttcccggt tcgccgcgat gcggattatc
     6301 agcaatttgc aggtgaccaa ttttatcggc ccactgagtc atggtattag ttaattcacc
     6361 ttccatccgc tgcatgtgat aaatgtcata ctgaattttc aaattgcagc aaccaacatc
     6421 gtcaatcagt ttcagtgcct gccgggttcc ggtgaaatgg aaaccaggaa tatcaaaatg
     6481 gttaataggt tcaatcagta ataaaatatc ttctttcatc agcatattcg cagcataacg
     6541 caggttttct acaagcgttg tgtgaatctg ttcactgctg aaaccagtcg gcgttttacc
     6601 gaccagacag ttaatttttt tattacccag agcacgagca taacgaatcg ctgctgctac
     6661 gccatcccga aactcttctt cacggccagg aatacaggca ataccccgct cgcccgccgc
     6721 ccagtcacct gccggtaaat tgtgcagcgt atgttcgagt ttattactcg ccagcacctg
     6781 ttttaattct tcaatgtcgt agtcataagg gaacataaat tcaacgccgc gaaaaccaca
     6841 ctgcgcagct ttctcaaaac gggcgagaaa atcatattca ccaaataaca tcgataaatt
     6901 agcagagaaa cgtaacatta tttatctccc ttattcatag tgcatgaaac aggcttcagt
     6961 cggtgcatcc gctgcgttat cggcgatatc ttcaaattcc atgacgttat ccagttcact
     7021 gcccatcgaa atattggtca cacgctcgag aataacttcc acgactaccg gtacccgata
     7081 ttgcgccatt aagattttcg cctgttcaaa ggctggtgca atatcttccg gtttgaaaac
     7141 gcgaattgct ttacaaccta aaccttccgc tacttttacg tggtcgacgc cataaccgtt
     7201 cacttcgctg gagttaatat tttcgaacgc gagctgtacg cagtaatcca tatcaaaggc
     7261 acgttgcgac tggcgaatca gacccagata ggcgttattg accagtacat ggatgtacgg
     7321 aatgttgaac tgtgcgccaa cggctaattc ttcaatcagg aactggaagt cgaagtcacc
     7381 ggaaatcgcc accacattgc gctctggatc agcggcacaa acccccagcg ccgccggaat
     7441 cgtccagccc agcgggcctg cctgcccaca gttgatccag tggcggtctt taaagacatg
     7501 cagcatttgt gccgcagcga tttgtgacag gccaatggtg gtgacataac aaacatcgcg
     7561 gccaaaggct ttgttcatct cttcatacac gcgctgcggt ttcaccggca cgttgtcgaa
     7621 gtgggttttg cgcagcaaag tgcgtttgcg ctgctggcag tcggcgaccc attctttacg
     7681 acacggcaga cgacccgctt tttgcatctc ctgcgccact tcaaccagca gcgtcagcgc
     7741 cgctttagca tcagagacaa tgccgagatc cggacacagc acgcggccaa tttgcgtcgg
     7801 ctcaatatcg atatgaacga ttttgcgccc ttcggtgtat ttctctaccg aaccggtatg
     7861 acggttagca aaacggttac cgataccaaa caccatgtcg gacgccagca gcgttgcgtt
     7921 accgtaacga tgcgcggttt gcagacccac catcccggcc atcagttcat gatcgtcggg
     7981 aatacagccc cagcccatca gcgttgggat caccggaatg ctggtcagtt cagcaaactg
     8041 ttgtaacagt gcagctgcgt cagcattgat taccccgccc ccggcaacaa tcaccggacg
     8101 ttcggcctgg attaacattt ctaccgcttt ttcgatctgc atacggctgg cagcaggttt
     8161 gtagaccggc agcggttcgt acatgtcagg atcaaactcg atttccgcaa cctgaacgtc
     8221 gaacggtaaa tccaccagta ccggacccgg acgaccagaa cgcatcaggt gaaatgcctg
     8281 ttgcagcacg cgaggcacca gcgccgcttc acgtactgta accgacattt tgctgaccgg
     8341 tttagcaatt gcttcaatat caacggcctg gaaatcttct ttatgcagac gggcgcgcgg
     8401 agcctggccg gtaatacaca gaataggaat ggaatcagca gaagcagaat agagcgcggt
     8461 gatcatgtca gtgcctgctg gtcccgaggt tcccagacat acgccgatat tccctgccgt
     8521 tgcgcgggta taaccttccg ccatgtgcga agcaccttcc acatgacgcg ccagaatgtg
     8581 acgaataccg ccgtgcttac gcatcgctga atagaaagga ttgattgctg ctcccggaac
     8641 gccgaaggcg gtggtgatac cttctttctc cagcacatac attgccgcgt caacggctct
     8701 catttttgcc attttattcc tacctctatt aattggaaaa attttccaac tttcaaattt
     8761 tgtcaaaaaa acccgctccg cagagcggga tgcgacagac actatggatg tgctttcagt
     8821 cccaacgccg tgctgatatc gcgggcggtg tctctgacca gctcaccctg actgacaaaa
     8881 cgatcttctg tcagtcttga tgaaggcccg gagatggaga tagcggcaac aacactacct
     8941 acatcatcgt aaattgctga agctatgcaa ttcaggccga caacatgctc ttctttatct
     9001 acggtatagc ccagttcacg cgcttgttcc aggtccttca gcaaggtggg catatccaca
     9061 agcgtagttg gcgtaaactg ctgcaaaccg gtttgcagaa tgatgctcat caactcctct
     9121 tccgccagcg gataaagcag cgccttgccc gcaccggaag catgcagcgg cagacgactg
     9181 cccagcggcg cacacatcct gaccatcgat ttacactcta actgaccaat taataccgct
     9241 tcattgccgt tacggatcgc gacattgacc gtttcgccgg aaagtaacat caggcggcgc
     9301 ataaacggcc cggcgacgga gagtacatcg cggttatgga tgtacgctgc accgacgtta
     9361 aagacaccta atcctatatg ccaccagcct aactgactgt cctgatagac aaaatccgct
     9421 gcctgtaaaa ccttcagcaa gcgaaaggtc gtggagagcg gcaaatccag attgagagaa
     9481 atatcgctaa ccgacgaact tcccccactt ttttccagat attgcagaat cgcaattccc
     9541 cgctctaacg cctgtgcgcc cttctgtgcc acaggctccg cctgtcctgg cctgccgcgc
     9601 cgtctaactt ccgtcatacc tacgttaact ccgacctaca agtgagcata aacaatgaac
     9661 aattcgttta tcttatgcaa gtcggttgca ggcgtcacgc aaaacagagt tcctgttcag
     9721 gaatactttc aacatcacag ttgtcactgc cgccgcgatc gatggtcaga aaatcggtga
     9781 cgcgctgcca ggcgaaaagt gggtgatgcc agacgttacg atggtaattc accccctgtt
     9841 caccgttggt aataaacgcc cgcagcgttg acaggtctgg tttgtcgtca cctaacgcca
     9901 cgactaccac aaacacttca cctttcatcg ggataaacgc ctgtgtaccc aacggatgac
     9961 gttcgagttc gtgaatagtc agcggcagat tcgccggttg cgcgcggtta atgctgataa
    10021 gcgtacggtc ttgctcaaga atctcaacca gcgccaaatc gtggtaacgc tctaccaggc
    10081 cattgttaat atggaaaaaa tcccgttgct gcgtttcgat tacgtcgcca taagcactaa
    10141 aggcttcctg acttaacggt aatacctgaa gtttcatctt tttgtccttg taaca
//
LOCUS       DAZKGF010000174         9988 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZKGF010000174 DAZKGF010000000
VERSION     DAZKGF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.174"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..223
                     /locus_tag="ACHOQV_004173"
     CDS             <1..223
                     /locus_tag="ACHOQV_004173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632459.1"
                     /translation="TQVIGITAPSGTILPNGSNIMQFTLPDVNGSVTLNVVASVSTDF
                     INGAFTIDNRAGNTLTVIAGSHLSVTLSN"
     gene            complement(367..552)
                     /locus_tag="ACHOQV_004174"
                     /pseudo
     CDS             complement(367..552)
                     /locus_tag="ACHOQV_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
     gene            complement(706..1863)
                     /gene="intS"
                     /locus_tag="ACHOQV_004175"
     CDS             complement(706..1863)
                     /gene="intS"
                     /locus_tag="ACHOQV_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFP9632460.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDANVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWIDEKVE"
     gene            complement(2025..2099)
                     /locus_tag="ACHOQV_004176"
     tRNA            complement(2025..2099)
                     /locus_tag="ACHOQV_004176"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2064..2066),aa:Arg,seq:cct)
     gene            complement(2175..3107)
                     /gene="yfdC"
                     /locus_tag="ACHOQV_004177"
     CDS             complement(2175..3107)
                     /gene="yfdC"
                     /locus_tag="ACHOQV_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP9632461.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKTIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3401..4156
                     /gene="mlaA"
                     /locus_tag="ACHOQV_004178"
     CDS             3401..4156
                     /gene="mlaA"
                     /locus_tag="ACHOQV_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFP9632462.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4218..5558)
                     /gene="fadL"
                     /locus_tag="ACHOQV_004179"
     CDS             complement(4218..5558)
                     /gene="fadL"
                     /locus_tag="ACHOQV_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFP9632463.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIRQSPAWLTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNPAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            5930..6214
                     /gene="yfcZ"
                     /locus_tag="ACHOQV_004180"
     CDS             5930..6214
                     /gene="yfcZ"
                     /locus_tag="ACHOQV_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFP9632464.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6395..7705
                     /gene="fadI"
                     /locus_tag="ACHOQV_004181"
     CDS             6395..7705
                     /gene="fadI"
                     /locus_tag="ACHOQV_004181"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFP9632465.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLLDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEMDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            7705..9849
                     /gene="fadJ"
                     /locus_tag="ACHOQV_004182"
     CDS             7705..9849
                     /gene="fadJ"
                     /locus_tag="ACHOQV_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFP9632466.1"
                     /translation="MEMASAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIANCKTAQEAEALARQGQQLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRMCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPQSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSGGYDAEARAFGEL
                     VMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDRQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDTALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
BASE COUNT         2442 a         2390 c         2544 g         2612 t
ORIGIN      
        1 gacgcaagta ataggaatta ctgcgccaag tggtactata cttccaaatg gcagcaatat
       61 tatgcaattc actcttcctg atgttaatgg aagtgttact ttaaatgtag ttgcctcagt
      121 ttctactgat ttcattaatg gagcttttac catagataac agggctggga atacattaac
      181 tgttatagct ggctctcatc tttcagttac tctatcgaat tgataaatat aataaaccta
      241 gtgttaatta ttatcactag gtttattttc tcactcgcat ttcttactaa aatcaattac
      301 aaatacagtt ccattatatt ttatgtcgta taatgctgta gaggatatgc tatcaaatga
      361 tcaataagtc gatgctttga atgtgaattt tgcattcgcg aagaagctaa aactcatagc
      421 cacaacgaaa cctgcgaagt tcgccatagc ttgatttgta tgcgctgcat ggatacaaac
      481 accaaaaaac acccagtgta aaagggtgtt cagcacacca atagaggtgt actttgcaaa
      541 tagctttaac atttcttttg tcagctaata atcaaaggca tgaagtctat catccaagtc
      601 tcaatcgatc gatgacttgc tatggttgat gagacaaaac tgagacaaac aaagctttgc
      661 actggattac aaggctttgt gatattcgat agttgttaag gcggatcact ccaccttttc
      721 atcaatccag tccgcccacc attgcatcat ttctctgcgc ttatcgagat actgtgcatg
      781 gttgtaaatc ccgcgcacag atccgccgtt ggcatgtgcc agttgcactt caatagcatc
      841 agcaggccat tcgtgctcgt tcataatcgt gctgaattca tgcctgaatc cgtgaccgct
      901 ttccagacct tcatagccga tttgtttgat cacaagtagc accgcgttct cgcagattgg
      961 ctttttctta tcgttgcgtc cggcaaaaac aaactctgat actggtttag tgatggagct
     1021 tagcgtagtg agaagttcaa ccacctggtc cgacatcgga accacatgaa tcttgcgtcc
     1081 cttcatcaca ttggcgtcga tggtgataat cctgttttca aaatcgacgt tcttccatag
     1141 catggaacga agctcttttg ttctgagggc tgtgtagcgt aaaactttgg tcgcaatgag
     1201 cgatacgata cttcctgaaa atgttgccag tgctttgtta aatgccggga tctggtctgc
     1261 aggaagaaac gggaagttct tcttgcggta tcccttcatg gcgtcagcaa ggtcaggtgc
     1321 cgggttatat ttagcccttc cggtgacaat agcgtaacgg aaaacctcgc cgcatcttct
     1381 tcgtgctttg ttggctcgct ccattgcacc gcgatcttca aatctgcgga ttacttccag
     1441 cagttgcatc ggctcaatat cctgaatttc aaggccgccg atgataggta aaatgtcgtc
     1501 atcaaacatt tttgcaagtt cagtcgcata ccctacagac cagacttgct tcttgtgctc
     1561 gtaccattcc ttgtaaatgg cgctaaagga attgttgtta gacgaagcct ttttcgcctt
     1621 taccggatcg atgccaaccg agatgtcttt tcttgcggtc catgccttat cccttgcttc
     1681 ctgcaaagtc ataagcggat attttccgac agtcaggatt ttctccttac cgtcaatctt
     1741 gtagcgaagc tgccatacct ttttccctga cacagggaca taaaggtaca ggccattacc
     1801 atcgagaagg cgatatggtt tttctttcgg ctttgctgct tcaatctgct taacggtgag
     1861 catgggtaaa aatccggggg gtaaaattat tttatccact ttttacccgt catggagtgc
     1921 ggctgtcaac gatctgacga gaaccatgac gaactgtgaa tctacggaag gcttgatatt
     1981 cagggtattt tgcggactgg tacggatggg agcgaactga taaatggtgt cccctgcagg
     2041 aatcgaacct gcaattagcc cttaggaggg gctcgttata tccatttaac taagaggaca
     2101 atgcggcatg agtatacccg ctaatggact gcggggtaag tacgctgccg ctcgattgct
     2161 taaaccctcg ccatttatgc cgggttttta tcatttttct taatgttttc cgcacgttct
     2221 gctttttggc gtgcttctgc tttgcgcttg ttgctcatgt cgttacggat ctgtgcatga
     2281 ctcattaacg cgaagataaa ggtgccgccg cagatgttcc ccgctaaagt aggtagtgcg
     2341 aagggccaga tgaaatcgct ccagtgcagc gtaccgttaa acaccagata gaggatttca
     2401 acagaaccga caacgatgtg agtggtgtca cccagggcaa taagccaggt catcaatata
     2461 atcaccacaa tctttgccgc acccgctgca ggaaacatcc aaaccatagt ggcgatcagc
     2521 cagccggaaa tgatcgcgtt ggcaaacatc tcgctggggg tgttcttcat cacatccatg
     2581 ccgattttga caaatgcatc gcgagtttct tcattgaaga ttggcatata ttcaaatgcc
     2641 cacgccgcaa tacctgtccc gagaatatta cccagcagca cgacgcccca taaccgcata
     2701 agtaagccaa cgttgctcat tgtcggtttt tgcatgacgg gtagtaccgc agtcacggtg
     2761 ttttcggtaa ataattgctg gcgggccata atgacgataa taaaaccaaa ggtataaccg
     2821 agattctcca gcaagaagct gcccggcacg ccttccagtt cgacatgaaa tatccctttt
     2881 gccagtagcg aagcgcccat cgacagaccc gccgcaatgg ctgaccacag tagtgccatt
     2941 gcgtcgcgtt ccagctcttt ttcaccatcc tggcggatat gctcatgaat tgccatcgcc
     3001 cgggagggga gtcgatcttc atctatttct attgttttgc cgcgctcttt ttcttcgctc
     3061 tcaacttcaa tttcgtcgct gtgttgatca attttgtcgt tgtccatggt ctcttctctt
     3121 gaattagcac gtatagctaa agcgtagcgg cttttttgct cgcaactggc gggagttact
     3181 ctgaaaatgt agaaaaggct gcgtttgcct ttttctgttt ctatagaatc aagtagccta
     3241 cagggcggcg attaccaggc tatgatcaaa tcagcaaatc agggcgtctg gacatcagtt
     3301 gacgtgctgt tacaatcgcc cacacctaaa caggcggata cggtatcgtt ccgtcatgga
     3361 tggcaaactg cataagccat aaaaaaacag ggagacattt atgaagcttc gcctgtcggc
     3421 gcttgctctg ggaactacgc ttctggtggg gtgtgcgagt tccggtacag atcagcaagg
     3481 gcgttctgac ccgctagaag ggttcaaccg caccatgtac aacttcaatt tcaatgtatt
     3541 agacccgtat attgttcgac cggtcgctgt cgcctggcgt gattatgttc cgcaaccggc
     3601 gcgtaacggt ttgagcaact ttactggcaa ccttgaagaa cctgcggtga tggttaacta
     3661 cttcttgcag ggcgaccctt atcaggggat ggtccacttt acccgctttt tcctgaacac
     3721 cattttgggg atgggcggct ttattgatgt tgcagggatg gcgaacccga aactgcaacg
     3781 gactgaacct caccgcttcg gtagtacgct tggtcattat ggtgtgggtt atgggccata
     3841 cgttcagtta ccgttctacg gtagcttcac gctgcgtgat gacggtggtg atatggcgga
     3901 tggtctttac ccggttcttt cctggctgac ctggccgatg tctgtgggta aatggacgct
     3961 tgaagggata gaaactcgtg cgcagttgtt ggattccgat ggtctgctgc gtcagtcgtc
     4021 cgatccttat attatggtgc gcgaagcgta cttccagcgt catgatttca tcgctaatgg
     4081 cggcgaactc aaaccgcagg aaaatccgaa cgcgcaagcg attcaggatg atttaaaaga
     4141 tatcgattct gaataagaaa caaacaaaaa aggtgagtcg caaaactcac cttttttctt
     4201 attaagcgaa cgcgttatca gaacgcgtag ttaaagttag taccgaacag ccaggcttta
     4261 ccttcagact cgaactggta tgggccttcg ttaattttca cgctctgacc gtgcatataa
     4321 gaaacaccaa catcgactga agcatcttta ttaaatgcgt aagtcgtacc tgcactcagc
     4381 cagaaacggt cctggtccgg aatggagata gaacgattct gtgccggaac tgggctgtca
     4441 tcaaaggcga taccggtacg gaaggtccag ttatcatcgt agtaataagt ggttcccaac
     4501 gcgatgcggt aagcatcttt aaagccttca tgtttctgga acagcgtgtc gccactcgtt
     4561 gaggtcgctt tcagctgctg gaactgactc cagctggtgt aagccaggct atagtgaatt
     4621 gcccactgcg gatcaacacg gttataaccc gacacttccc acatttcagg caggttcagc
     4681 gtcagataac ccgactgcgt tgcgccacct gtcgcggtag gaattggcaa accgtagtta
     4741 ttaaaagccg ggttaagatc gctgctgtag ttacccttga agtcaatttt cacttcagag
     4801 cggtaggtca atgcgtagcg gttgttttta tccagttcat acaggatacc ggcgttccag
     4861 ccaaagcccc actggttacc gttcaggtga gcgattttgg tattactgtc gatgccgttg
     4921 gcggtagctg ctaatgcttg cccctgagga gtcagccaag caggagattg cctaatctga
     4981 ccagccacca gttgccccag atcgcctgcg aaacgttcaa ttttcgcgcg agcgtagacg
     5041 gcgttgaaac caagaccaaa gctccatgca ttatttaagc gatatgcacc gcttaagttc
     5101 aggttcatgg tttcgaggtc ggtggtacca ccgacagaac cgcctgcata agtatcgtta
     5161 aactctgtcg ccagaccata gttagaggta attgaagccc cccaaccaaa ttggtcgtta
     5221 atcggtgcaa caaagtgcat gttcggaacc catgccgtag gcgcgatgtt atcggctttc
     5281 aggctacgac cagacggaga cgttccgctg atatttacat ccggatcaat ataaaccgca
     5341 cccgcagaaa atgtcgggcg gtcaaacatg gtaatcaatg cggggttacg gctaacgtta
     5401 cctgcatcat cggcaattgc accttcccct gaataagccc ggcccaggcc agaggaagaa
     5461 aattcgttta actgaaagcc tgccgaccag gcctgggtgg agataagtgc cactgcgact
     5521 gcgagagcag actttgtaaa cagggttttc tggctcatga ccataacctc aatgatttat
     5581 ttttatacaa actatgttac gtgctgtaac aggagcgcga agtgtagggt ctgaggtaca
     5641 tcttagaaat cagaccagtg gcgagagtat aggtcggacc agctggaatg ttgcaagtat
     5701 gtttctatct tttttcagat atgatctaag aaacgcgatc cagctggcaa aattgggagg
     5761 tggcgcactc atggattcgg gtgaattttg ttttagatca tttttaagtg tgatttcggt
     5821 cacttatccg attttgcaga attaacaact ggagcagcac tttccggaag cgtaaaatat
     5881 ccacaacgtt tatctggcac cttgggtgca catacagagg aaatcaacga tgagtaaatg
     5941 tagtgctgat gaaaccccgg tttgctgctg tatggatgtt ggcaccatta tggacaactc
     6001 cgactgcacc gcgtcttaca gccgtgtgtt tgcaaaccgc gcagaagcag aacaaacgct
     6061 ggcggcgctg actgaaaaag cccgtagcgt ggaatccgaa ccgtgcaaaa tcaccccgac
     6121 tttcactgaa gagtctgacg gtgtgcgcct ggatatcgat ttcacttttg cctgcgaagc
     6181 tgaaatgctg atcttccagc tgggtctgcg ttaattcctc tcgggtacat acgcccctgt
     6241 cagttgctgg caggggcgtt ttcttttctc ttctactttt ctctctgtgt catagctccc
     6301 actttccctt ttctccactt ggctaaatgt aaaaaaatgg ttaagactat gatcaggtca
     6361 gaccacttta tttattattt tacaggggag tgttatgggt caggttttac cgctggttac
     6421 ccgccagggc gatcgtatcg ccattgttag cggtttacgt acgccttttg cccgccaggc
     6481 gacggctttt catggcattc ctgctgttga tttggggaag atggtggtag gcgaactgct
     6541 ggcacgcagc gagatccccg ccgaagtgat tgaacaactg gtttttggtc aggtcgtaca
     6601 aatgcctgaa gcccccaaca ttgcgcgtga aattgttctc ggtaccggaa tgaatgtgca
     6661 taccgatgct tacagcgtca gccgcgcttg cgctaccagt ttccaggcga ttgcaaacgt
     6721 cgcagaaagc ctgatggcgg gaactattcg cgcggggatt gccggtgggg ccgattcctc
     6781 ttccgtattg ccaattggcg tcagtaaaaa actggcgcgc gtgctggttg atgtcaacaa
     6841 agctcgaacc atgagccagc gcctgaaact cttctctcgc ctgcgtttgc gcgacttaat
     6901 gccggtgcct ccggcagtgg cggaatactc tactggcttg cggatgggcg acaccgctga
     6961 acaaatggcg aaaacctacg gtattactcg cgaacagcaa gatgcattag cgcaccgttc
     7021 gcatcagcga gccgctcagg cgtggtcaga cggtaaactc aaagaagagg tgatgactgc
     7081 ctttatccct ccttataaac aaccgcttgc cgaagacaac aatattcgcg gtaattcctc
     7141 gcttgctgat tacgcaaagt tgcgtccggc gtttgatcgt aaacacggga cggtaacggc
     7201 agcaaacagt acgccgctga ccgatggcgc agcggcggtg atcctgatga ccgaatcgcg
     7261 ggcgaaagaa ttagggctgg taccgctggg gtatctgcgc agttacgcat ttactgcgat
     7321 agatgtctgg caggacatgt tgctcggtcc ggcctggtca acgccgctgg cgctggaacg
     7381 tgccggtttg acaatgagcg atctgacatt gctcgatatg cacgaagcct ttgcagctca
     7441 gacactggcg aatattcagt tgctgggtag cgaacgtttt gctcgtgatg tactggggcg
     7501 tgcacatgcc actggcgaaa tggacgatag caaatttaac gtgcttggcg gttcgattgc
     7561 ttacgggcat cccttcgcgg cgaccggcgc gcggatgatt acccagacat tgcatgaact
     7621 tcgccgtcgc ggcggtggat ttggtttagt gaccgcctgt gctgcgggtg ggcttggcgc
     7681 ggcaatggtt gtggaggcgg aataatggaa atggcatcag cgtttaccct taatgtccgt
     7741 ctggacaaca ttgctgttat caccatcgac gtaccgggtg agaaaatgaa taccctgaag
     7801 gcggagtttg cctcgcaggt gcgcgccatt attaagcaac tccgtgaaaa caaagagttg
     7861 cgcggcgtgg tgtttatctc cgcgaaacca gacaacttca ttgctggcgc agacatcaac
     7921 atgatcgcca actgcaaaac ggcgcaagaa gcggaagcac tggcgcggca gggccaacag
     7981 ttgatggcgg agattcatgc tttgcccgtt ccggttatcg cggctattca tggcgcttgc
     8041 ctgggcggtg ggctggagtt ggcgctggcg tgccacggtc gcatgtgtac tgacgatcct
     8101 aaaacggtgc tcggtttgcc tgaagtacaa cttggattat tacccggttc aggcggcacc
     8161 cagcgtttac cgcgcctgat aggtgtcagc acagcattgg agatgatcct cactggaaaa
     8221 caacttcggg cgaaacaggc attaaagctg gggctggtgg atgacgtcgt tccgcaatcc
     8281 attttactgg aagccgctgt tgagctggca aagcaggatc gcccatcttc acgccctcta
     8341 cctgtacgcg agcgtattct ggcggggccg ttaggtcgtg cgctgctgtt caaaatggtc
     8401 ggtaagaaaa cagaacacaa aactcaaggt aactatccgg cgacagaacg catcctggag
     8461 gttgtcgaaa cgggattagc gcagggcacc agtggcggct atgacgctga agcccgtgct
     8521 tttggcgaac tggtgatgac gccgcaatcg caggcgctgc gtagtatctt ttttgccagt
     8581 acggacgtga agaaagatcc tggcagtgat gcgccgcctg cgccattaaa cagcgtggga
     8641 attttaggtg gtggcttgat gggcggcggt attgcttatg tcactgcttg taaagcgggg
     8701 cttccggtca gaattaaaga tatcaacccg cagggcataa atcatgcgct gaagtacagt
     8761 tgggatcagc tggagggcaa agttcgccgt cgtcatctca aagccagcga acgtgacaga
     8821 cagctggcat taatctccgg aacgacggac tattgcggct ttgcccatcg cgatctgatt
     8881 attgaagcgg tgtttgaaaa tctcgaatta aaacaacaaa tggtggcgga agttgagcaa
     8941 aattgcgccg ctcataccat ctttgcttcg aacacgtcat ctttaccgat tggcgatatc
     9001 gccgctcacg ccgcgcgacc tgagcaagtt attggcctgc atttcttcag tccggtggaa
     9061 aaaatgccgt tggtagaaat tattccccat gccggtacat cggcgcaaac catcgccacc
     9121 acagtaaaac tggcgaaaaa acagggcaaa acgccaattg tcgtgcgtga caaagccggg
     9181 ttttacgtca atcgcatctt agcgccttac attaatgaag ctatccgcat gttgaccgaa
     9241 ggtgaacggg tagagcacat tgataccgcg ctggtgaaat ttggttttcc ggtaggccca
     9301 atccaacttt tggatgaggt aggaatcgac accgggacta aaattattcc tgtactggaa
     9361 gccgcttatg gagaacgttt tagcgcgcct gcaaatgttg tttcttcaat tttgaacgac
     9421 gatcgcaaag gcagaaaaaa tggccggggt ttctatcttt atggtcagaa agggcgtaaa
     9481 agcaaaaaac aggtcgatcc cgccatttac ccgctcattg gcacacaagg gcaggggcga
     9541 ctctccgcac cgcaggtagc tgaacggtgt gtgatgttga tgctgaatga agcagtacgt
     9601 tgtctggacg agcaggttat ccgtagcgtg cgtgacggag atattggcgc ggtatttggc
     9661 attggttttc cgccatttct cggtggtccg ttccgctaca tcgattctct cggtgcgggt
     9721 gaagtggttg caataatgca acgactggcc acgcagtatg gttcccgttt taccccttgc
     9781 gagcgtttag tcgagatgag caagcgtggg gaaagttttt ggaaaacaac tgcaactgac
     9841 ctgcaataag aaggtcaaag ctatatgaat ccgcgctgaa tggcggagtg ttgctcaaaa
     9901 tgtaaacgca tattgactat acttacgcca ttgaggtaaa aaacagcgtt tcattcggtg
     9961 aatggataag gcacaatgcc ggccaccg
//
LOCUS       DAZKGF010000175         9985 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZKGF010000175 DAZKGF010000000
VERSION     DAZKGF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.175"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1112..3301
                     /gene="zorA1"
                     /locus_tag="ACHOQV_004183"
     CDS             1112..3301
                     /gene="zorA1"
                     /locus_tag="ACHOQV_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorA1"
                     /protein_id="HFP9632467.1"
                     /translation="MSWLNSILVTLTSVEPYKVPVTVIVTVTFAFVCFIFFYLLRSIR
                     IIYGLKKYTRSINSIEKSAPEVQLEHLKSLFQRSELKHAWNEFEESLHSQYELENGEE
                     KIVRIRATAPSASFFSEQQLVDIPLNTEFFKHLPGILTGMGIIGTFYGLMIGLNHFDP
                     STPEQVSSSVNNLLRDVLYAFLGSAFAIFASILVTWLEKLSIAKSYKYLEKFTAALDS
                     LYDSGVGEEYLASLVKSSNESATQARHLKESLVTDLRDMLLHLAESQKIENERLANTL
                     SATYRESGSQFADQVSGAIENSLKSPLDKIAGAVQTASGDQSGMVQNMLQNVLTAFMA
                     KLDTTFGQQFTNLNEMMGQTVGAIQTMQTGFSALLQDMRQVSDDSRQGSAQLIEQLLS
                     EMKSGQQALQAGMNDMLTSLQVSVAKIGAEGEGAGERIARQLEKMFADSEAREKAQAE
                     HMAAFVEAIQNSVQQGQSATMEKMAASVGALGEQLGSLFGQIDKGQQQISATQQANQQ
                     SLHEQTQRVMSEVDDQIKQLVETVASQHQGTTETLRLLAEQTNRQIQDMQAGADKMRL
                     AAERFEHAGERVSEANHLTADVLNKAQSAGSSLSLATSELTSVVADYRNNREAVSKSI
                     AMLELLAANTQSEQTTRNQFIADLKQHGERLQSYNREAQVFMENVSDVLGKGFEDFSE
                     GVSRSLDKTLGKLDVEMAKASNLLAGSVEQLGESVSELDDVLSRVRT"
     gene            3315..4055
                     /gene="zorB1"
                     /locus_tag="ACHOQV_004184"
     CDS             3315..4055
                     /gene="zorB1"
                     /locus_tag="ACHOQV_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorB1"
                     /protein_id="HFP9632468.1"
                     /translation="MFGNAFGVKKRRSDEAEKPFWISYADLMTAMMVLFLVVMVASLS
                     SVTQRIQRAEQGEKARGQDISRLCERLELHARNVNKNIVVDCHDNRISFGEAGRFAHN
                     QFFLNAEGQKALQDVVPLVLEASNSEEGKKWFKQIVIEGFTDTDGSYLYNLHLSLQRS
                     EWVMCSLLDSRSPLQKNISAEQQLQIRKLFLAGGVSFNNAKESKEASRRVELRMQFFG
                     LKDKRDKADEVDFPPVVNKEVCQLVMPL"
     gene            4052..5734
                     /gene="zorC"
                     /locus_tag="ACHOQV_004185"
     CDS             4052..5734
                     /gene="zorC"
                     /locus_tag="ACHOQV_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorC"
                     /protein_id="HFP9632469.1"
                     /translation="MIPALNSLSQRIAARLSSSQRDDHYLHNDFHALTAATLDMEKRF
                     DRAERIPSPPQEMRLEALRRFRLAQELTEREWRMVFYGLADNDPLYPDQPILLEDDTF
                     FPRVNSAIKKRIETKTLKRRDWAALCSSYFAYQHQSPETNPHWCVLRGHIAQGYLVVK
                     AAIRREKSWMKTIEFYHDIFTPQAGGVISRQLLAGENNSLSSLEKIAQIPDSSWLWKR
                     IFTVLLAQLDTLDDPQFLSKISWLLGLAAQWVRFRDDIMTATLTRYYHSGYRDQSHPA
                     LKQAALEYWDNPQLKSQQNKWHQYVSESVAAMVRGWLAKQDLTHFFELLRGNGDVDQA
                     RLHYWLRFANQMGFTRIIMGSDAWQDRGSDFVKFREENKGRLSYLRGGRNFDNAMVMQ
                     INDYLFVEFSGTGNAMYAYQIGHAPFNPESRTLDINIHLKDQGRCALRLPHAPRAEGY
                     NKVRITGWMLKYDDELRKLGIRWMAEEPVRFVDKKVPPPVAMSDIKIINPLRDTAIQR
                     LVKCSSCIVSDNRHKGGILSVQLITPDDTVERELLRLGFAPVAKEPHRYWIK"
     gene            5734..8976
                     /gene="zorD"
                     /locus_tag="ACHOQV_004186"
     CDS             5734..8976
                     /gene="zorD"
                     /locus_tag="ACHOQV_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorD"
                     /protein_id="HFP9632470.1"
                     /translation="MLKRLLSKLTGNRQQIEHHLKNQYQVEENGLSFPLSLVDDSQLW
                     ALASWLEQLAEEDYLISLTDRWLLSWEALYRLLEDEEHASSLPLIGVPDILPLRASLS
                     SRGALSDSDFRVWIAEWATFPARKPIRFSRTGAILTHDNQQYLLSRENWALLQATEQL
                     SAQQIQTPGETTNQLGWAAIRKCAKLAAAKFDDYLEKTHVIKPTSLSLRLRKATVADT
                     AVIEIEPHFEDQPANWLGSFDKNLQVHDSYRIPGENGELSHVIIPPEVKEVLNSIHSI
                     PGRRVAGSEALSFVRNPYTFLGEDAASVIAPEEHEQALFDARIFFHHFRLIPQLNTEN
                     KITEVTLILEPVSPVPQPEITFVFSAPWELDKFIQQLGISVAAQMPAGSWQGYELELS
                     QFTEQQWHDCQALLTRWQQEIEGKEFSDVLDIAKYGDRVIGIGEFEKISSPWLTKAQS
                     ENWLPDGIDFSAFSIETLSGWQPENPLHFDELQEIITQAEAAGETYITTPWNDSQLPL
                     DAAKTFSKNWEKQQITANEYQGNVADKTARAVLKIEQNIEETAYIKQRRNSLLNARHA
                     EPEIPLSLKEHIRLKDHQREGVAWLQQLFLRSPEETAGCLLADDMGLGKTLQILSFLV
                     WFIEKFPQEPPNLIVAPVSLLDNWERELNNFFYTAGIPVLKLYGETIKAVKYPKQAIP
                     AHLQSKGIKNLLKPGWQGEAKIILTTYETLRDQEFSLARQPWSIMVCDEAQKIKNPAA
                     LITHAANAVQARFKVACTGTPVENTLVDLWSLFDFAQPGLLGALNEFGKQYVRPIENE
                     DGRDTERLESLRALIEPQTLRRTKEEVARDLPQKIEVESCKQLTLSGVQKQLYLSSVA
                     NWQQQQALREGMQQAGTGMLGLLHRLKLICAHPAIVNPEPRFRDNSPKLNWLLKILAE
                     IKHTSKDKVIIFTELRDLQRELQHAIHQNFGFRPVIINGDSSTKSQSQNSRQRLIDDF
                     QAQPGFGVIILSTVAVGFGVNVQKANHVIHFTRCWNPAKEDQATDRAYRIGQTKNVYV
                     YYPTVRDTEITTFEETLDDLLQRRRALARDMLCATPDLNCADFETILKGA"
     gene            complement(9136..9302)
                     /locus_tag="ACHOQV_004187"
                     /pseudo
     CDS             complement(9136..9302)
                     /locus_tag="ACHOQV_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(9553..>9985)
                     /locus_tag="ACHOQV_004188"
     CDS             complement(9553..>9985)
                     /locus_tag="ACHOQV_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632471.1"
                     /translation="TPSFGYSCKHSMCIEIYRLLSETQTMLAGYYWVMEYTPDKGLHI
                     HFIGYLDGQRHKNSYQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKS
                     AVDDLRYALSYLAKQDQKEHGIILGRSRLPEKSNRGRPRHN"
BASE COUNT         2843 a         2363 c         2320 g         2459 t
ORIGIN      
        1 tacagcaata tggtagattt atcgatcgat ataacacaaa tgaacagttc tttattctgc
       61 tatatatcgc aaaaacagca cattgcaaaa tttataacca taattaatgg aaaagcgcag
      121 aaaattattc cactgaatgc ttttaataca gttggttagc ttaaaaaata gcaacttttg
      181 atttcaatca ttgaaaattg caatgcgtga cgattcaaca gcatttaagt gagtttcagg
      241 atatgggaag gacatgaaag gaggagcgta ataaaacaca ccagaagaag gaggagaaaa
      301 tgcttccctg tatatttatt ttgtcaggcc atggcctttc tccagcaacc gatcaacgcc
      361 acatacctgc agcgacactc aacagaagtg agatcccatg caatatcatc atcacagggc
      421 cagcggaagg tcccttgtac gggcggtagg tacacagcca tatgccatgt ttatcctggc
      481 acaggacttt tatgcaagta taggctttgt tgatgcagaa ccatcatacc atgcaaagct
      541 aagttcgttc tggacatact gtatcaggat gccaatcccc gacttccatc gacaggttta
      601 cgatcaggaa tacattatca accgtcagta ttgcgaaact ctcagaagac atctgaaaac
      661 atatttcaga agagaatccc tgttctacat acttttagtc ggtagcctca tgcaggctcg
      721 cactttaccg gcagcattcc ataatttgtg atcctactca ttaagaaatc gttatcaggt
      781 attgttcatg aaaatcctca ggatggatca caacggaaga gtaatcatat cttatcgcag
      841 attcagattt agaatgcggc acgtaaaata ttgtccaggg gacgctgacc acgacacaac
      901 atcaagaaat ttgcttttgc atggtaaaaa agaggaaact aagaaaatgt ctcacctgat
      961 tattctgttt ctatcgttca tttattgaac agtaagagta ttcaggtgta accgcacgac
     1021 atagtgacaa ccaatgcaac ataatcaatt atgttgacta tgttgctgat gactaatttt
     1081 agtatggata atatattaaa cgagtaaaac aatgtcctgg cttaattcca ttttagtaac
     1141 acttacatct gtagaaccct ataaggtacc tgtcactgtt atcgtcactg ttacctttgc
     1201 ctttgtctgc tttattttct tttatttact gagatccatc agaattattt atggattaaa
     1261 aaaatacacg cgttcaatta acagcattga aaaaagtgcg ccagaggttc agcttgaaca
     1321 ccttaagagc ctgtttcagc ggtctgaact caagcatgcg tggaatgagt tcgaagaatc
     1381 gttgcactca caatatgaac tggagaacgg cgaagagaaa atcgtccgta ttcgggctac
     1441 tgcgccgagc gccagcttct tctcggagca acaactggtc gatattccgc tgaacactga
     1501 atttttcaaa cacctgcccg gtattctgac cggtatggga attatcggta ccttctacgg
     1561 tctgatgatt ggtctcaacc acttcgaccc cagcacacca gagcaggtct ccagtagcgt
     1621 caataatcta ctacgcgatg tactgtacgc cttcctgggt tctgcatttg ctatttttgc
     1681 ttccattctg gtcacctggc ttgaaaaatt atccatcgct aaaagctaca aatatctgga
     1741 aaaattcacc gcagccctcg actccctgta tgacagcggc gttggcgagg agtaccttgc
     1801 ctcactggtg aagtccagta atgaaagcgc gacccaggca cgccatctga aagagagcct
     1861 ggttacagat ttacgcgata tgttactaca tctggccgaa agtcagaaaa tcgaaaatga
     1921 acgcctcgca aatacgttaa gcgcaactta ccgcgaatca ggttcacaat ttgccgacca
     1981 ggtcagcggt gcgattgaaa acagcttgaa gtcaccattg gataaaattg ccggtgcggt
     2041 tcagaccgcc agcggcgatc aaagcggcat ggtacaaaac atgctgcaga atgtactgac
     2101 cgcgtttatg gcgaaactcg acaccacctt tggtcagcag tttacaaacc tcaacgaaat
     2161 gatggggcaa accgttggtg caattcagac catgcaaacc ggtttctccg cgttattaca
     2221 ggacatgcgt caggtgagcg acgattcccg ccagggcagc gcgcagctta tcgagcaact
     2281 gttatcagag atgaaatctg gccagcaagc tctacaggct ggcatgaatg atatgctcac
     2341 cagccttcaa gtctcagtgg cgaaaattgg cgcagaaggt gaaggggccg gtgaacgtat
     2401 agctcgccaa ctggaaaaaa tgttcgctga tagcgaggcg cgagaaaaag cccaggcaga
     2461 acatatggct gcttttgttg aagccattca gaattcagta caacagggac aaagcgccac
     2521 aatggaaaaa atggcagcct ctgtcggggc gcttggtgaa caactgggta gcctgtttgg
     2581 acagatcgat aagggtcagc aacaaatttc agcgactcag caagcgaatc agcagtccct
     2641 gcacgaacag actcaacggg taatgagtga ggtagacgat cagattaaac aattggtcga
     2701 aactgttgcc agccagcatc agggaacaac agaaactctt cgtttgctcg cagaacaaac
     2761 taaccggcag attcaggata tgcaagctgg cgcggacaag atgcgtctgg ctgctgaacg
     2821 ctttgaacat gccggtgagc gggtgtcgga agcaaaccac cttaccgctg atgtgctgaa
     2881 taaagcgcaa tcggcaggtt catcactttc ccttgccacc agcgagctca cctccgttgt
     2941 ggctgattat cgtaacaatc gggaagcggt tagcaaatcc attgccatgc tggagttgct
     3001 cgccgcaaat acgcagtcag aacaaaccac gcgcaaccaa ttcatcgcag atcttaagca
     3061 acacggtgag cgtctacaga gctataaccg ggaagcacag gtctttatgg aaaatgtaag
     3121 tgacgtgctc gggaaaggat ttgaagactt ctctgaaggt gtttcacgca gcctggataa
     3181 aacgctgggt aaactggacg tcgaaatggc gaaagcctct aatctgctcg cgggatctgt
     3241 cgaacagctc ggagaaagcg tcagcgaact tgatgatgtt ctgtcacgcg tacgaaccta
     3301 aggggtgatg gctgatgttt ggaaacgcat ttggcgttaa aaaacgccgc agcgatgaag
     3361 cagaaaaacc attctggatc tcctatgccg atctgatgac tgcaatgatg gtgttatttc
     3421 tggtcgtcat ggttgcgtca ctgagttccg ttacacagcg tattcaacgt gcggagcaag
     3481 gcgaaaaagc aagggggcag gatatttcca gattgtgtga acgcctggaa ctgcatgccc
     3541 gcaatgtgaa caaaaacatc gtagtggatt gccacgataa ccgtatcagc tttggcgaag
     3601 ccgggcgttt cgctcataac caatttttcc tgaacgccga ggggcaaaaa gcgttgcagg
     3661 atgtagttcc tcttgtactg gaagcgtcca acagtgaaga gggtaagaag tggtttaagc
     3721 agatcgttat tgaagggttt accgacaccg acggttcgta cttatacaac ctacatctct
     3781 ccctgcaacg ctctgaatgg gtaatgtgta gtctgctcga tagccgcagc cctctgcaaa
     3841 aaaatatctc cgcagaacag cagctccaaa tccgtaagct tttcctcgcg ggtggcgtct
     3901 ccttcaataa tgctaaggaa agcaaggaag ccagccgccg ggtcgagttg cgcatgcagt
     3961 tcttcgggct taaagataaa cgtgataagg ccgatgaggt ggattttccc cccgtcgtta
     4021 ataaagaagt ttgccagttg gttatgccac tatgataccc gccctgaatt ctctttctca
     4081 acgcattgca gcgaggctct cttccagcca aagggatgac cattatctgc ataatgattt
     4141 ccacgcgttg accgcggcta ctcttgacat ggaaaagcgg tttgataggg cagaaagaat
     4201 cccctcaccg ccacaagaaa tgcggctgga ggctctacgt cgctttcgcc tggcacaaga
     4261 actcacggaa cgagagtggc ggatggtgtt ttatggcctc gccgataatg atccattata
     4321 tcctgaccag ccgatcctgc tggaggatga tacatttttc cccagagtaa acagtgcaat
     4381 caaaaaacgt attgaaacca aaacgttgaa acgccgggat tgggcagcac tttgttcaag
     4441 ctattttgcc taccaacacc aatcacccga gacaaaccct cactggtgcg tactgcgggg
     4501 gcatatcgct cagggttacc tggtagtgaa agccgctata cgacgagaaa aatcgtggat
     4561 gaaaaccatt gagttttatc acgacatttt taccccacaa gcgggaggag tgatctccag
     4621 gcagttactt gcaggtgaga acaattcact ctcatcatta gagaaaatcg cgcaaatccc
     4681 tgatagcagt tggttatgga agcgcatttt cactgtgctt ttagctcaac tggacacgct
     4741 cgacgatccc cagtttctga gcaaaattag ctggttgtta ggcttagctg cgcagtgggt
     4801 gcgtttccgt gatgacatca tgactgcaac cctgactcgc tattaccatt ctggctatcg
     4861 cgatcagtca catccagcgt tgaagcaggc cgcgctggaa tactgggata acccccagtt
     4921 gaaaagccag caaaataaat ggcatcagta tgtctccgag tcggttgccg caatggtccg
     4981 gggctggttg gcaaaacagg acctgacaca tttctttgaa ctgctgcgcg gtaatggcga
     5041 tgtcgatcag gctcgactgc actactggct gcgtttcgct aatcagatgg gatttactcg
     5101 tatcatcatg gggtccgatg cctggcaaga tcgtggtagc gattttgtga aattccgcga
     5161 ggagaataag ggacgattga gttacttacg aggtgggcgt aactttgata acgccatggt
     5221 tatgcaaatc aacgattatc tgtttgtcga gttttccggg acaggaaatg cgatgtatgc
     5281 ctatcaaata ggtcacgcgc catttaaccc tgaatcacgc acgcttgata tcaacattca
     5341 tctaaaagat caggggcgat gcgcactcag actcccccat gcacctcgtg ctgaggggta
     5401 taacaaagtc cggataaccg gctggatgtt gaaatacgac gatgagctac gtaagttggg
     5461 cattcggtgg atggctgaag aacccgtcag gtttgtcgat aagaaagtgc cccctcccgt
     5521 cgctatgtct gacatcaaaa tcatcaaccc gttgcgtgat acggctatac aacgcctggt
     5581 taaatgtagt tcatgtatcg tcagcgataa tcgacataaa ggtggcatac tgtccgtaca
     5641 actcattaca cctgacgaca ccgtagaaag agagttgtta cgactaggct ttgcacctgt
     5701 agctaaagaa ccgcatcgtt actggatcaa ataatgctga aacgtctgtt aagtaaactg
     5761 acgggaaacc gtcagcagat agaacaccac ctaaagaatc agtatcaggt tgaggaaaac
     5821 ggtctcagct ttccgctatc gctggttgat gattcacagc tttgggcact ggcatcctgg
     5881 cttgaacaac ttgctgaaga agactatctg atatcactta cagatcggtg gttattgagt
     5941 tgggaggcac tatatcgcct gctggaggat gaggaacatg ccagcagcct gcctcttatc
     6001 ggtgtacctg acatattgcc cttacgcgca agcctgagtt cacgaggagc attaagcgat
     6061 agcgattttc gcgtctggat tgctgaatgg gctacatttc cagcccgcaa gccgattcgt
     6121 ttcagtcgta ccggtgccat tttaacgcat gataatcagc aatacctgtt atcgcgggaa
     6181 aactgggcgc tcttacaggc aacagaacaa ctcagtgcac agcaaattca gactcccggc
     6241 gaaaccacta accagctggg atgggccgct atccgcaaat gtgcgaaact cgccgccgcc
     6301 aaatttgatg attatttaga aaaaacgcac gtcatcaaac cgacttcatt gtcattacgt
     6361 ctgcgtaaag caacagttgc cgataccgca gttatcgaaa ttgaacctca tttcgaggac
     6421 cagcctgcta actggctggg cagttttgac aagaacttac aggttcacga tagctaccgc
     6481 atcccaggag agaatggcga actcagtcac gtcatcatcc ctcccgaagt gaaagaagtc
     6541 ctgaattcaa tacactcaat cccagggcgt cgagtggccg gaagtgaagc gctttccttt
     6601 gttcgcaatc cttatacctt cctgggtgag gatgccgcca gtgttattgc ccctgaagaa
     6661 catgaacagg cactgtttga tgccaggatt ttctttcatc atttcaggtt gataccgcaa
     6721 ctgaatacgg aaaataagat cacagaagtt acgctgatac ttgagcctgt ttcacctgtt
     6781 ccccagccag aaatcacttt tgtgttctca gcgccctggg agctggataa attcatacag
     6841 caactcggta ttagcgtcgc tgcacagatg cctgccggtt catggcaagg ttacgagctg
     6901 gaattaagtc agttcaccga acagcagtgg cacgattgcc aggcactgct aactcgctgg
     6961 caacaagaaa ttgaaggaaa agaatttagc gatgtgctgg atattgcgaa gtatggagat
     7021 agagtgattg gcattggtga atttgaaaaa atatcctctc cctggctgac caaagcgcag
     7081 agtgaaaact ggcttcctga tggcatcgat ttctccgcgt tttcaatcga aacactatct
     7141 ggctggcaac ctgaaaatcc tctccatttt gatgaactac aggagataat tactcaggca
     7201 gaagccgctg gtgaaacgta tatcaccaca ccatggaatg acagccaatt accgttggat
     7261 gccgctaaaa ccttcagtaa gaactgggaa aaacagcaaa tcactgctaa tgaatatcaa
     7321 ggcaacgttg cagataaaac ggcccgagcc gtactcaaaa ttgagcagaa tattgaagaa
     7381 acggcctaca tcaaacagcg ccgcaattca ctcctcaatg cgcgtcatgc cgaaccagaa
     7441 attcctctga gccttaaaga gcatatccga cttaaagacc atcaacgtga aggcgtagcc
     7501 tggctccagc aactcttcct tcgttcacct gaggaaacag caggctgcct gctggcggat
     7561 gatatggggc ttggaaaaac gctgcaaatt ctgagcttcc tggtatggtt cattgaaaaa
     7621 ttcccgcaag aaccgccaaa tctcattgtt gctcctgtct ctcttttaga taactgggaa
     7681 cgtgagctga acaatttctt ctataccgca ggaataccag tattgaaatt gtatggcgag
     7741 accattaaag cggtgaaata tcccaaacag gccatccctg ctcatctgca gtctaagggg
     7801 atcaaaaacc tgcttaaacc tggctggcaa ggtgaggcaa aaatcatcct gacgacctac
     7861 gaaacgcttc gcgaccagga gttttctctg gcacgccagc catggtccat tatggtgtgc
     7921 gacgaggcac aaaaaataaa aaacccagct gcgttaatca cacatgcggc caacgcggta
     7981 caagccaggt ttaaagtggc atgcacagga acgccagtcg aaaacacact tgtcgatctg
     8041 tggagcttat ttgattttgc ccagcctgga ttgttagggg cactaaatga atttggtaaa
     8101 cagtatgttc gccccatcga gaacgaagat ggccgcgata cagaacggct ggaaagcctg
     8161 cgggcgctga tagaacctca gactttacgc cgaaccaaag aagaggttgc acgtgattta
     8221 cctcagaaaa ttgaagtaga aagctgcaag caactgacgt tgtctggcgt gcaaaaacag
     8281 ctctaccttt cgtctgttgc aaattggcaa caacagcaag cactgcgcga agggatgcag
     8341 caggccggaa ccggtatgtt aggtttgtta caccgactga aactcatctg tgcacaccct
     8401 gcaatagtta atccagagcc acgttttcgt gataactcac ccaagcttaa ctggctgctg
     8461 aaaatactcg cagaaataaa acatacctcg aaagataagg tcattatttt tacggagctt
     8521 cgagatttac agcgcgagct tcaacatgca atccaccaaa attttggttt ccgccctgtc
     8581 attatcaatg gtgatagcag cactaaaagt caaagccaaa acagccgcca acgcctgatt
     8641 gatgattttc aggctcagcc tgggtttggg gtgattattc tctctacggt tgctgtcggg
     8701 tttggcgtta acgtccagaa agccaatcat gttattcatt ttactcgctg ctggaatcca
     8761 gccaaagaag atcaggctac ggatcgggcg tatcgcattg ggcaaactaa aaatgtgtat
     8821 gtctattacc caactgtgag agatactgaa atcacaacat ttgaagaaac actggatgat
     8881 ctcttacaac gccgacgagc attggccaga gatatgctct gcgctactcc cgatttgaac
     8941 tgtgcggatt ttgagaccat tttgaaggga gcctaaccga tatgagcggc aatactatca
     9001 gttgccaaac ctgaaagaac acttagctgt gatgattcgc tattacctac cgatagcttc
     9061 ggaaaaacat aaattaaaaa tgcccagagg agtactatta tcgttagtat tattatccga
     9121 acgctgtatt aacggttatt tattggtgag tactacgttc catggcaaga gttcatcaac
     9181 aggttggaag accattccgg caaaactctc agtatatggc gcagatacca ttcagacagc
     9241 aggtcccgct cagcccgtac agtagagcgc cgttcactac cgtaatcgct accgaagaac
     9301 acattgattg cccctcaata atcctgctgt taacatcaaa attgttggtc gggttcgatt
     9361 tatttaattg tgtaaacagg gtctctcaga gaaaggccct tatgcatcac ctgacgttaa
     9421 aagtcactac acccaagatg acgttcaaag tgtcatagtc ccctacactg atgacaaagc
     9481 cgccctctgc tgcctattaa ctcaagcacc tcccaacaac ttacagaaac ttaattctaa
     9541 tacagctgta attcagttat gtcgtggtcg accccggtta cttttttcgg gaagcctgct
     9601 acggcccaga ataataccgt gttctttctg atcctgtttc gccagatagc taagggcgta
     9661 acgtaagtcg tctaccgcag atttatcact atagtgaatt acatgatcga tacgcaccgg
     9721 gtatttgtct ttagcccggc acaggtgaaa ataaccctcc ccttccgtga tccgcctcca
     9781 gatatccccc agttgcctgg atatctgata agagttcttg tgacgttgcc catccagata
     9841 gccgataaaa tgaatatgaa gccctttgtc tggtgtatat tccattaccc agtaatatcc
     9901 tgctaacatc gtctgggttt cgctaagtaa acggtatatt tcaatacaca tactgtgctt
     9961 acaggaatac ccgaaactgg gcgtg
//
LOCUS       DAZKGF010000176         9872 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZKGF010000176 DAZKGF010000000
VERSION     DAZKGF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.176"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(69..794)
                     /gene="phoU"
                     /locus_tag="ACHOQV_004189"
     CDS             complement(69..794)
                     /gene="phoU"
                     /locus_tag="ACHOQV_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP9632472.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(809..1582)
                     /gene="pstB"
                     /locus_tag="ACHOQV_004190"
     CDS             complement(809..1582)
                     /gene="pstB"
                     /locus_tag="ACHOQV_004190"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9632473.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1673..2563)
                     /gene="pstA"
                     /locus_tag="ACHOQV_004191"
     CDS             complement(1673..2563)
                     /gene="pstA"
                     /locus_tag="ACHOQV_004191"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP9632474.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIAAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2563..3522)
                     /gene="pstC"
                     /locus_tag="ACHOQV_004192"
     CDS             complement(2563..3522)
                     /gene="pstC"
                     /locus_tag="ACHOQV_004192"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFP9632475.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALTVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3609..4649)
                     /gene="pstS"
                     /locus_tag="ACHOQV_004193"
     CDS             complement(3609..4649)
                     /gene="pstS"
                     /locus_tag="ACHOQV_004193"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP9632476.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4897..5967)
                     /locus_tag="ACHOQV_004194"
     CDS             complement(4897..5967)
                     /locus_tag="ACHOQV_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9632477.1"
                     /translation="MNKYIIPWCFIVFIFSFSGATFAAPKGICTSDNGSFHSTLDFSG
                     YLITADENRVGKTFNKTVTNGDSYPAHCHCDTGNVGEFPYIYYTARINESLGYAGVRS
                     NVNYFNLNPNLDVGISIDLLGVGYINAPFEYHANRPTGGSYKCSRTDPLSISSGAKAI
                     IYFYIKKTFAGKLIIPETLVAKLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEINS
                     LRPINFDFKEIPAADFSSVVGSTVTTHKITKTVTIECENLGILNTDDISTSFYATEPS
                     TDNSMVVTSNPNVGIKIYDKNNKEINVNGGELPTDMGKSTVFGEKAGSVTFSAAPASL
                     TGARPAPGTFTATATITIEIVR"
     gene            complement(5978..8500)
                     /gene="lpfC-O113"
                     /locus_tag="ACHOQV_004195"
     CDS             complement(5978..8500)
                     /gene="lpfC-O113"
                     /locus_tag="ACHOQV_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFP9632478.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQSLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFVAIKKTLTTFGVK
                     VDALKSLNDVDETVCIDPGPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPSRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTTDGS
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQVQILHGFPYGFTLYGGMQA
                     AEKYGSAALGVGKELGALGAISFDVTHARAKFSHADTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNNPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGTYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTRVGYRVLFRVAGTKGKPAPFGAIATVQNTSSADSGIVGDQGELYLSG
                     LPEKGQVMLSWGENAATTCTFDYSLSIPESESGLIEQGVTCH"
     gene            complement(8525..9256)
                     /gene="lpfB-O113"
                     /locus_tag="ACHOQV_004196"
     CDS             complement(8525..9256)
                     /gene="lpfB-O113"
                     /locus_tag="ACHOQV_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFP9632479.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLVQPALTYAGVVIGGT
                     RVVYLSNNPDKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPAQEK
                     VLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKAANSLEIAVKTRIKLFWRPASIRL
                     IPEDAAPKVKWHREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(9304..>9872)
                     /gene="lpfA-O113"
                     /locus_tag="ACHOQV_004197"
     CDS             complement(9304..>9872)
                     /gene="lpfA-O113"
                     /locus_tag="ACHOQV_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFP9632480.1"
                     /translation="RNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSDTA
                     DQTVPLGKVSKNAFSGVGSLASPQQFSIKLEKCPATYTQAAVRFDGTEAPGGDGDLKV
                     GTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEMKF
                     IARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         2530 a         2507 c         2316 g         2519 t
ORIGIN      
        1 ttttaggatt ttataaagtt atatataaca aatcccaata attaaattat tgggatttac
       61 ttggtgaatt atttatcgct atctttttct gccagcagtt tatcgagctc gtcaccaccg
      121 acgtgacgga aatcctgccc cttcacgtag tagaagataa actcacaaat attctggcag
      181 cggtcgccga tacgttcgat tgaacgcgca caaaacagcg cggtaagtac gcttggaatg
      241 gtgcgcgaat cttccatcat gtaggtcatc agctgacgaa caataccttc gtactcctga
      301 tcgacttttt tatcttcacg ataaatacgt accgcttcat caatgtccat ccgcgcgaac
      361 gcgtccagca cgtcgtgcag catctggatg gtatgacggc ccagcgactc cagacttacc
      421 agcaatggct gatgctgctg ggagaatttc tccagtgcag tacggcagat tttgtccgcc
      481 acgtcgccaa tacgctccag ctcggcaatg gttttactga tcaccataac cagacgcagg
      541 tcgctcgccg tcggctggcg tttggcaata atgcgcacgc aggcttcatc gatcgccact
      601 tccatcatgt tgacgttctt gtcgccttcg atgacgcgct tcgccagatc gctgtcctgg
      661 ttgtgcattg cagtgatcgc atcagaaagc tgctgctcca ccatgccgcc catggtcatc
      721 acctgcgtgc ggatgctttc cagttcggcg ttgaactggc cggaaatatg tttattaaga
      781 ttgagactgt ccataacgca ctcctgaatc aaccgtaacg accggtgatg tagtcttctg
      841 tttgtttctt cgctggcttg gtgaacagat cgtccgtgtt gctgaactca atcaattcgc
      901 ccaggtacat aaacgccgtg tggtcggaac aacgcgcagc ctgctgcatg ttgtgggtga
      961 cgatcaccac ggtgtaatcc tgcttcagtt cggtaatcag ctcttcaata cgcccggtgg
     1021 agatggggtc gagcgccgaa catggttcgt cgagcagcag cacttccggg cgaatggcga
     1081 taccacgcgc aatacacaaa cgctgttgct gaccaccaga gagagagtaa ccgctctggt
     1141 gcaatttatc tttggtttcg ttccacaatg cggctttggt caatgcccac tgcacgcgct
     1201 cgtccatgtc agcacgggag agcttctcaa acagacgcac gccaaaagcg atgttgtcgt
     1261 agatggacat cgggaacggc gtcggtttct ggaacaccat gcccactttc gcacgcagca
     1321 gggcgatatc ctgagagttg gtcaggatgt tgtcgccatc gagcagaatt tcaccttccg
     1381 cacgctgttc cgggtacagt tcaaacattt tgttgaaggt acgcagcagc gttgatttac
     1441 cgcagccgga cggcccgata aacgccgtaa cctggttttt ggcgatatcc aggttgatgt
     1501 ttttcagggc atggaatttg ccgtagtaga agttcaaatt acgaacctga attttactcg
     1561 gggcagtttc aaccatactc attgcaatct tttcctcatc gtgcctgatg cacttcgcga
     1621 atcaggctta ccgtaggccg gataaggcgt ttacgccgca tccagcaaag tgttaaccgt
     1681 gtttattctt cgcaaaaaca acgcgcgcca gaatgttcag cagcagtacg cacagggtaa
     1741 tgatcaacac cccggcccag gccagttgct gccattccgc aaacgggctc atcgcaaact
     1801 taaagatcgt caccggcagg ttggcgatcg gctgcatcat gtccgtgctc cagaactggt
     1861 tggagagcgc agtaaacagc agcggcgcgg tttcaccagc aatacgggca atcgccagca
     1921 ggataccggt catgatcccg gacaccgacg ctttcagcgt gatcgcggca atcatcttcc
     1981 acttcggcgt acccagcgca taagccgctt cacgcaggct gtccggcacc agtttcagca
     2041 tgttctcggt ggtgcggata acaatcggca cctgcaacag cgccagcgca atcacgcccg
     2101 cccagccgga gaagtgctcc atctgcgcca ccacgatggt gtaaacaaaa agaccaacca
     2161 caatcgacgg tgcggagagc aaaatgtcgt taatgaagcg aatcacttct gccagccagg
     2221 atttacgacc atattcagcc agataaatcc ccgccataat gcccagcggc gtaccgaata
     2281 ccgtggccca caaaattaac aggccgctac ccgccagggc gttcgccaga ccaccgcctt
     2341 ccgtatttgg cggcggcgtc atttcggtga acagcgccag cgacatacca tcgataccgc
     2401 gagtgatggt ggacattaaa atccagatca gccagaacag accgaaggcc atcgtcgcca
     2461 tcgagagcgt cagcgcaata cggttcttaa ggcggcgacg cgcctgcatt ttacggcggg
     2521 attcagccag cgccgcagtg gtttgcattt caaccatagc cattagcgtg ccccctcatt
     2581 cttagccagg cgcataatca taaacttcga tgcggcgagg acgatgaagg taatcacaaa
     2641 caggatcagg cccagttcca tcagtgcagc aacatgcaga cccgactctg cttccgcaaa
     2701 ttcgttggcc agcgcagagg tgatactgtt gcccggcata tacagcgagg cgctgtcgag
     2761 ttggtaggtg ttaccgatga taaaggtcac cgccatggtt tcaccgagcg cgcgccccag
     2821 ccccaacatg atgccgccga taacaccatt tttggtgaac ggaagaacga tacgccatat
     2881 aacttcccag gtggtgcagc caataccgta ggccgactct ttcatcatca ccggggtttg
     2941 ttcgaataca tcgcgcatta ccgccgcaat gtacggaata atcatgatgg cgaggatcac
     3001 gcctgccgcg aggataccga taccaaaggc cgggccggag aacagcgcgc cgacaatcgg
     3061 gatattcgac atgatattgc cgaccggctc ctggaagtaa acggcgaaca gcggggcgaa
     3121 gataaacaag ccccacatgc cgtaaacgat acttggaatg gcagccagca gctcaatggc
     3181 gatacccagc gggcgtttca gccagccagg cgcaagctcg gtcaggaaca gggcgatacc
     3241 aaaactcacc gggacggcga tcagcagcgc gataaacgag gtcaccagcg taccgtagat
     3301 cggcaccagc gccccgtaga tatcgttcgg cgcatcccac tctttggtcc acaggaaagc
     3361 cagaccaaat ttctgaatgc tcggccagga tgagatgatc agagagacaa taatgccacc
     3421 caacatcaat agcacagtca gcgccgccag ttttaccagc acgctgaaaa ttatgtcgcc
     3481 ctttttaccc ggtgggttaa aagcaggctt ggttgcagcc ataagttact cttcagttaa
     3541 acgcgtttac aaagtcattt tgtaatgccg gatgcggcgt aaaacaccgt acccggcctg
     3601 gagttttatt agtacagcgg cttgccgcta ctgtctttaa tattggtctt ccacgcagcg
     3661 cgaacctgtt caactacgct atccggcagg ctggcataat ccaggtcgtt cgcctgttta
     3721 gccccggttt tgtatgccca gtcgaagaac ttcagcactt cggtgccttg ttctggtttc
     3781 ttctgatctt tatggatcag aatgaacgtg gtagaggtaa taggccatgc atcttcgcct
     3841 ttctggttgg tcagatcctg agcgaatgtt ttgctccagt ctgcgccttt tgctgcatta
     3901 gcgaagtttt cttcggtcgg actcaccggt ttgccatcag cggagatcag tttggtgtac
     3961 gccaggttgt tctgcttcgc gtaagcatat tcaacgtaac caattgcacc cggcagacgc
     4021 tgaacgaacg cggcgatacc gtcgttacct ttaccgccca gaccgatcgg ccattttacg
     4081 gtagagccag taccaacgtt gtttttccac tcttcgttca ctttcgccag gtagctggtg
     4141 aagacgaagg aagtcccgga gccatctgcg cggcgtacta cagcaatgtt ctgtgaaggc
     4201 agtttcagac ccggattcag tttggcgatg gcttcatcat cccacttctt gattttgccc
     4261 aggtagatgt cgccgagggt tttaccatcc agcaccagtt cgccagactt cagccctgga
     4321 atgttaaccg ccagcaccac gccgccaatc acggtcggga actggaacag gccttcctga
     4381 gccagttttt cgtcagacag cggcgcgtca gaggcaccga aatcaacggt attagcgata
     4441 atctgtttta cgccaccgga agaaccgata ccctggtagt taactttatt accggtttct
     4501 ttctggtaag tgtcagccca tttggcatac accggcgcag ggaaggttgc acctgcacct
     4561 gtcaggcttg cttctgcaaa cacagagaaa gcactcatcg ataaggtcgc ggcgacaaca
     4621 gttgcgacgg tggtacgcat aactttcata atgtctcctg ggaggattca taaagcattg
     4681 tttgttggct acgagaagca aaataggaca aacaggtgac agttatatgt aaggtttatg
     4741 acagttttat gacaaatcag aattattaat taactttttg attttattga ctaagaaaaa
     4801 gaaaagtaat gtcatctttt tgtcatgcag agtgaaaact cgcggacaaa aatgcgtaag
     4861 atgacaacgg tgtgaggaac accgctgtca gttcgtttag cgtacaatct caattgttat
     4921 cgttgcagtc gcggtaaatg ttcctggcgc ggggcgagcc cccgtaaggc ttgcaggagc
     4981 agcagaaaaa gtgacgctac cggctttttc accaaagacg gttgatttac ccatatctgt
     5041 cggcagttca ccaccgttaa cattgatttc cttattattt ttatcataaa ttttaatccc
     5101 aacgttcggg tttgatgtca cgaccattga gttgtcagta ctgggttcgg tagcataaaa
     5161 agaggtactg atatcatcag tatttagtat tcctaaattt tcacactcaa tggtgacggt
     5221 tttggtaatt ttatgcgttg tcacggtact tccaactacc gaagaaaaat ctgccgcagg
     5281 aatttcttta aaatcaaaat taatgggtct taaactgtta atttcacagc tttgcggtgc
     5341 agtgatatcg ccacgaatat aaacatccgc cataggttgt gagtaatcaa ccggagtgtc
     5401 acggcttatg gttccgtaca atttcgccac gagagtttca ggaataatta actttccagc
     5461 aaaagtcttc ttaatataaa aataaattat tgcttttgca ccactggaaa tacttaatgg
     5521 gtccgtgcga ctacatttat atgaacctcc agtaggcctg ttagcatggt attcaaaagg
     5581 tgcattaatg tatcctacac cgagaaggtc tatcgatatt ccaacatcca gattaggatt
     5641 caaattaaag tagtttacat tagaacgaac acctgcataa cctaaggact cgtttattct
     5701 cgctgtataa taaatataag gaaactctcc cacgttccct gtatcacaat ggcagtgagc
     5761 aggataagag tccccatttg tcacggtttt attaaaggtc tttcccactc tgttctcgtc
     5821 tgccgtaatc agatagccgg aaaaatcaag tgtgctatga aaggacccat tatctgaagt
     5881 gcagatacct tttggggccg caaatgttgc gccactaaag gagaagataa aaacaataaa
     5941 gcaccatggt ataatatact tgttcataaa aatcctttta atgacatgtc acaccttgtt
     6001 caattaagcc gctttcactt tctggtattg aaagtgaata atcgaaggtg catgtagtgg
     6061 cggcattttc tccccaggag agcatcacct gccctttttc aggaaggccc gagagataaa
     6121 gctccccctg gtcaccgaca attcctgaat cagcggagct tgtattttgt actgtagcaa
     6181 tagcgccaaa aggtgctggt ttaccttttg tacccgcaac cctgaaaagc acacgataac
     6241 caacacgagt aacaaattcg gccttaacca ctgcaccacg ggtgggaacc atcgtaacga
     6301 cggtattatc aagttctata ttcgacttcg agaaatagtt atcgctgatt gctacccggt
     6361 tttcatcata agggtttagc tgtgtcttga ccgtatagcc gcgccagtcc gtcgcagcac
     6421 cgcgcatatt gtctatttct aacccggcag cccctggtgc tttaacaaga gctatggttt
     6481 cacccaactc ctggcttaac gttacgcctt cgctgtgcgc cagaattccc cctctgatac
     6541 cataattcag cctctgagaa ttttggctgt agttatagct gccgttcacc gtgccataag
     6601 tgccatcata ggtcagggac atattaccgc caacctcatc atcatggctg ttataggact
     6661 gcattaaatt ccaggaaagg ttatttcttt ccagcgccgt accgctgagt ccaagttgat
     6721 gtgagctacc ggaattatcg ttttgtgaaa cagaataggt ggcgtaacta tttccccacc
     6781 agcccgataa aggaacagat aaagagagca tgtagatctg ctcactggtg ttatcactgg
     6841 catggttgtt ccccgtcccc tgccgggcag tattggaata actccaggag acgttccacg
     6901 agagacgctt ccagctactg ctgtagccaa attgcattaa tcgttcaaca tcatcggtat
     6961 gccagtactg ctggcgactt aatgtcagat ataagttgcc atagtctctg cccagtgatt
     7021 gcgttaacgt tccttccaca cggctgcggc ggttaccggt ttcccagaaa tcttcaggat
     7081 tattacgccg cgacgcccat tcattgagag tatagtaacc ttcggtggag taacgatagc
     7141 caaccaggcg caagctggta tccgtatcgt caaagcgttt cgaatacaga aagcgataag
     7201 actgaccggt ttccgtatca gcatggctaa atttcgcacg ggcatgtgtc acatcgaaag
     7261 aaattgcacc cagtgcgcca agctctttac cgacacccaa agcggcagaa ccatactttt
     7321 cagcagcctg catacctcca tacaaggtga aaccgtaggg aaaaccatga aggatctgca
     7381 cctgaagaac gtcaggggta aagccatctt catcgcgtaa ttctcccaca ctgacatcaa
     7441 catcagtttg accttcacgt ttgaggatgg ctaacgacgc ataaggttga gtaaatcgtt
     7501 gttcgctacc gtcttcttct ttgatggtta cgtccagatc accacctgta ctggcggtgt
     7561 tgaggtcggt aatttcaaaa gcaccttgtg gaacggctga ctgataaatc acgtagccat
     7621 tctggcgaat aatgacagtg gcgttagact ttgcaatccc acgcactacg ggggcaaatc
     7681 cgttttggct ggcaggcaac atatcgttat cggtatacaa gcgtgcgcca cgaatttgcg
     7741 tactgtcgaa gatatcgctc gccgtccacg tatcacctat cataatctgg cttcttaacg
     7801 cagctatatc acgttgtatc caggtgctta tagagttgta ttctgcactg ccatcggtcg
     7861 tatttaaggt gctgtaattg cgtaatcgcc acggcccaat attcagtcca ttgcgcagat
     7921 tgagataact ggtatctgtc tcttgtgaac cataatccga acgtagagta tgtgaaccag
     7981 aaaaatcgta gttgatggtg agcgcgttaa tcccctcatc ccaacgcgag gggctgatgt
     8041 aatcataagc catcgcatcc atatagattt ggggaatgga gatattcagt tgcagtttat
     8101 cgctgtcgaa ctgccatgat gagccttcaa tgcgtggccc aggatcaata cagactgttt
     8161 catctacatc attgagcgat ttcagcgcat caacttttac gccaaaggtg gttagcgttt
     8221 ttttaatagc gacaaagcag gcgtgtaaat cagcatcggg attatcctga acaaaggtca
     8281 catcctgacg ttcaaacatt ttaccgttga taaaaatatc aacgctataa acaccagggg
     8341 gcatcaggtt tgcccgatta aactggcgaa tatcgactcc ggattgatgg tcgatttcca
     8401 gaagcgccgg attgaataca ctgacctcgg ccatcaggct ttgcgagaaa atcagacacg
     8461 tccctgccgt gaggccaacc actattctgg tcgtcatcat aatacgccta tccttatccc
     8521 ttgcttatag cgtcattttt atcgggtcac tgactgcgcc gtaatcatta ataaatcgcc
     8581 aggtcatttg acctcccgca gaattagcag gtaaaggtaa cgtgagcgtt tcaaaaggac
     8641 ggatcatgtt gaggggcaca tcatgaccat caacgatgac atccatcaca ctgatatgaa
     8701 tcgggttggg attctctgca atcaggtttc gcccttcccg gtgccacttc accttcggtg
     8761 cagcatcttc gggaatcagg cgaatgcttg ccggtcgcca gaacagcttg atacgcgttt
     8821 taactgcgat ttccagtgag ttcgccgctt tgttgctcgc agagggcgga atatttttaa
     8881 tattcagcca gaagactgac tcgcgatcat caggcaatga cacacctttg gtatgaataa
     8941 tgcgtaatac tttttcttgc gcgggttcta atcgtgatac cggaggaata acggtaaacg
     9001 gtgctttgct tttatcctct ttattaaacg gatctaccca tgcctggatg agataaggaa
     9061 ttttttcttc cttgctaaat acagaaatag atttatctgg gttattactt aagtaaacta
     9121 cccgtgtacc accaatgaca acgcctgcat aggttaatgc tggttggact aaaaaaacac
     9181 tgagtataat cgcggtaaat aacttacatg aaacggttgt tttaaagagc ctgattaaac
     9241 aactttttct ggacataacg actccctggc caaagccaac tgaataaaaa ggcccttgat
     9301 aaattatttc ttatattcga cagtaaattg tgaatcggcg ttcgccgtac ccgccgttac
     9361 ggttgcattg gttgccacat agcgtgcaat aaacttcatt tcagccttgc cttcagcgtc
     9421 gatagcggta taggcagaat cgttataaag tttaacctgc gagttatctg actggttaaa
     9481 aatacgaata ccgacacccg ttgccgcaat cgcttgtcct gtaccggtaa aatcgccagg
     9541 gttgcccgct gtaagcggcg tacccacttt caggtcgccg tcgccgccag gcgcttctgt
     9601 accatcaaaa cgaacggctg cctgagtgta ggttgccggg cacttttcaa gtttaatact
     9661 gaactgctgt ggtgacgcca gactacctac acctgaaaat gcatttttac tgactttacc
     9721 cagtgggacg gtttgatcgg ctgtatcgga ggtaacttca caagtcgtgt cgacaatttc
     9781 accgacgaag tgaacaacac catcttctgc cagtgcatgt ccggccagca ttaaagatgc
     9841 cagagtgaat gcaccgccta taatattacg ct
//
LOCUS       DAZKGF010000177         9855 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZKGF010000177 DAZKGF010000000
VERSION     DAZKGF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.177"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(170..1330)
                     /locus_tag="ACHOQV_004198"
     CDS             complement(170..1330)
                     /locus_tag="ACHOQV_004198"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP9632481.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(1336..2007)
                     /locus_tag="ACHOQV_004199"
     CDS             complement(1336..2007)
                     /locus_tag="ACHOQV_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFP9632482.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCIAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(2155..3636)
                     /gene="tolC"
                     /locus_tag="ACHOQV_004200"
     CDS             complement(2155..3636)
                     /gene="tolC"
                     /locus_tag="ACHOQV_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFP9632483.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            3841..4470
                     /gene="nudF"
                     /locus_tag="ACHOQV_004201"
     CDS             3841..4470
                     /gene="nudF"
                     /locus_tag="ACHOQV_004201"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFP9632484.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            4471..4893
                     /locus_tag="ACHOQV_004202"
     CDS             4471..4893
                     /locus_tag="ACHOQV_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFP9632485.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            4918..5745
                     /gene="cpdA"
                     /locus_tag="ACHOQV_004203"
     CDS             4918..5745
                     /gene="cpdA"
                     /locus_tag="ACHOQV_004203"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFP9632486.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            5745..6326
                     /gene="yqiA"
                     /locus_tag="ACHOQV_004204"
     CDS             5745..6326
                     /gene="yqiA"
                     /locus_tag="ACHOQV_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFP9632487.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            6355..8247
                     /gene="parE"
                     /locus_tag="ACHOQV_004205"
     CDS             6355..8247
                     /gene="parE"
                     /locus_tag="ACHOQV_004205"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP9632488.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFTLWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(8292..9161)
                     /locus_tag="ACHOQV_004206"
     CDS             complement(8292..9161)
                     /locus_tag="ACHOQV_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HFP9632489.1"
                     /translation="MNNKFKIILWMMLSALPALSYAQVKNIVTGEYSAVSKIITEKET
                     LYPSDKILLVFDIDNTLLTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISML
                     YELSPMQLTEPQLPTMLKQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRK
                     KEDTLPPFEKGKLKRSWLYSQGVFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQAN
                     IHAMTKMFSKDKWSSTDFTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLA
                     TLFPDRNKLCPIK"
     gene            complement(9175..>9855)
                     /locus_tag="ACHOQV_004207"
     CDS             complement(9175..>9855)
                     /locus_tag="ACHOQV_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFP9632490.1"
                     /translation="SPTLVCAEQPHGFIDYRHEYLDDTRTHADRVEFGTFFSNGIGLM
                     GELRYNTDEGDKDKWDPSQFGNNGTGLSVVYRFKPLDDKKFWLEPMFWLDTTQYWSTY
                     EYGLSAGYDFSKEWKVSGRFRYDMDKATDKSKGYGNDDRNNRRYDVWIDYRPQKTNFQ
                     YQLNLVYYNNGYLTWNDGHKNYTASFKVGYKMGSWIPYMSIADYKGVDKTSSNRQIRW
                     RWGLTYTF"
BASE COUNT         2357 a         2382 c         2564 g         2552 t
ORIGIN      
        1 tgtattcact tactttaatt acaatatcgc ttgcatatga cgatttaaca ttaaattgag
       61 tattaatgtt aaataaaggt tggatatgta tgtgtttata tattgatggg aattatgaat
      121 ggtggattat aagagattgc cccgcttatc ggggcaatat taagctgtat tactcaacaa
      181 aaatatgcgg ataaaaccga gacatatcct gggtgatcaa tgcacggtct tcacgaatgc
      241 caattccggc aggttgatcg ttcaccagcc agctaccaat cagcatatag ctgtcgccga
      301 atttcggtaa cgggtggaat tgctgaacaa tcatcccttc ttcgccatac ggaccttccg
      361 ctgcttcaat ggttttgccg ttctcaatga tcgacacgtt tgcgccttca cgggagaaga
      421 tcggtttaac cacatatttt tccatttgcg gatgatcatc ttccgcaaaa taagcaggca
      481 gcaggttcgg gtgattcggg aacatctccc acagtagcgg cagaagcgct ttgttggaga
      541 taatgctctt ccacgccggt tccagccagc gtacgcctgc atcctccagc ttggtggaga
      601 acatctcgcg cagcataaat tcccacggat acagcttgaa caggttggaa attacctgat
      661 cctgtaaatc cgtgaactga cctttttcgc ctaaaccgat atcgtcgatg tagaggaatt
      721 cggtagcgat ttcagcttcc gtcgcgcagt cctgcaaata ctgaatggtt ccgcgatctt
      781 ccaccgtgtc gcgacagcag gtgagatgca gcaactggaa gccatactgt tcacgcaact
      841 cgacaaagcg atcgattagc ttttcttgca gactgttaaa ctggtcgctg ccctccggca
      901 agttaccggc gttaagctga tcttccagcc agatccactg aaagaacgcc gcctcgtata
      961 gtgacgttgg tgtatcggcg ttattttcca gcagtttagg ttcgccagtg ccatcccacg
     1021 ccagatcaag acgcgaataa agcgatggct ggtgagtcag ccatgactga cgcacaaaac
     1081 tccaggtgtg ttttggaatg cggaatttgg tcatcagctc atcgctggcg atcacttttt
     1141 ccaccacttt caggcacatc tggtgcagtt cggcggtgac ttcttccagc ttttcaacct
     1201 gggcgagggt caacttgtag taagcatctt cacaccagta cggctcgccg tacatggtgt
     1261 gaaaattgaa accgtattcg tgggctttct cacgccagtc cgggcgctcg gtaatactga
     1321 ctctttccat cggtatcagc cacccattga acgagaagag gtgccggttg cgctacgctg
     1381 catagtgctt tgtttggcaa cagattcacc aaaaccgcca cgggtaacgg tagtggtggt
     1441 cgccggtttt ggtgccatgg ctgtcttcgg tacggtcatg gtgcggcctg gctgggctgc
     1501 gccatagttt ttacccgtcg cgtcggtata tttaccgtaa gccgggctgg ctgggttttt
     1561 cgaggagaac agcggctgct gtgcaaatcc cgcaccgccg cccatcagac gacccatcat
     1621 gtaaccggcc atcagcggca tccagaaact cccgctggat tgctgggcct gcgcctggtt
     1681 ttccggtgcc atgccagcct gggctggtgc ctgctggcac tgaccttcgc caaattcagc
     1741 aatacagtct tcacgagtgg cgtatttcgg cgcagtacgt tcggcttctt tcagcgcatt
     1801 gttgtacgcg gtggtacatt cggcgctttt gcctgggttt gcagctgaac agtcgtcagc
     1861 attttgatag agagacactg tttcatcact cttttcacag ccagccagca taaaaacagt
     1921 ggcaaccgcg agagcgactg gtgtcagatg gcgtgcgctc cagtttttgc ggaacgatgc
     1981 gtggcgtatg gattttgtcc gtttcatttt tgtcttccgg gaccagtggt aaatacccat
     2041 cagaatagag gatggctggt cgaaattgaa gcgagaaggg ggaagaatgc ggcagataac
     2101 ccgtatcttt acgttgcctt acgttcagac ggggccgaag ccccgtcgtc gtcatcagtt
     2161 acggaaaggg ttatgaccgt tactggtggt agtgcgtgcg gatgtttgct gaacgacggg
     2221 tgccgggcta tcaggcgcat aaccatcagc aatagcattc tgttccggcg tttgcggggc
     2281 aacgttttcc ggattagtgg aaaccggttt gctcagcgca ttgttcagtg ccagaagatc
     2341 ctgctcgttc aacgtaccca gagctgattt aatattcaac tggttaatca ggtagttata
     2401 acgcgcattc gccagctctt gcttggcgtt gtacagcgtg gtggtcgcat ccaacacatc
     2461 aacaatggta cgcgtaccga ccgagtaacc cgcttccatc gcgtctaatg agctttgagc
     2521 ggaaactacg gcttgtttgt aggcgttaat gctactgata gaagcattaa tgttgttgaa
     2581 ggaggaacgt acggtttgca cgacgctacg atgcgcgctt tccagttgct cgctggcacc
     2641 aacaaagttg tactgggcct gtttcacctg cgagttaacc attccgccct gataaatcgg
     2701 cagcgagaag ctcaggccaa ctttgttctg gcccatattg ctgtcgtcat actgggtacc
     2761 agcggcacca cgggttttcg aaccgctata agaggtgtca gaaatcccgg tagaagccgt
     2821 taaatccaga gtcggtaagt gaccatcctg cgcctggcga atttgctcgc gcgccaggtc
     2881 ctggctcaag cgcgcctgta acagcgacag gttgcgtttt tcggcttctt tcagcagcgt
     2941 gttaaccggc tgtggtttgt cggttttaaa gttttcgaca ttcagcgccg ccagttctgg
     3001 atagtagtta ccggtgatct ggcgcagctg ctctaccgcg ttatcaaggt tattacgtgc
     3061 ggtcacttcg ttcgccagca cggtatcgta ctgtgcgcgg gcgttctgca cgtcagtgat
     3121 cgctaccagg cccacgttaa aacgttgggt ggtttgatct aattgacggt agatcgcttc
     3181 tttttgcgcc tgtgtatagg aaagaacgtc aatagcattc aacacgttga aataagcggt
     3241 cgcggtgttg aggatcaagg tttgttgatc ggtctgatac gtgacgtcct gaatcccagc
     3301 tgctttttcc tgcagcgtta acgcacgcca tttcgacata tcaaaaatgg attgagttaa
     3361 ctgcagggac gcactggtcg cgttagagtt gatgccgttc gcgtcgcggt agccgttgct
     3421 ataggtgtaa tctgcaccta aacctagctg tggcagtaat ggactgcgcg cttcattaat
     3481 tttttcaaag gcagcatcac gatcggcggc agacttacgc aattccgggt tactaaggcg
     3541 tgcttgctga taaacttgca tcaggttctc ggcctggctc aacgaactga aaccagaaag
     3601 gctcaggccg ataagaatgg ggagcaattt cttcatttgc attccttgtg gtgaagcagt
     3661 atttagcgcg atcaaactgt aaaattattg cggattctag cagaagccgc taccgcaaaa
     3721 ggttggcgtt acgtgccata gggcgttaat gtgcctcaat ttaacacatg actgctcaaa
     3781 cggcagtcaa acgtcgctga aattcacatt taattcacta ttagtgccag gacattaaca
     3841 atgcttaagc cagacaacct gcccgttaca tttggcaaaa acgatgtaga aattattgca
     3901 cgagaaacac tttatcgcgg ctttttttca ttagatcttt atagatttcg tcatcgtcta
     3961 ttcaacgggc aaatgagtca tgaggtacgg cgggaaattt ttgagcgcgg tcacgccgca
     4021 gtcttgctac cctttgaccc agtgcgtgat gaagttgtgc tgattgagca gattcgaatt
     4081 gccgcgtacg acactagcga aaccccctgg ctactggaga tggttgccgg gatgattgaa
     4141 gagggtgaaa gtgtggaaga tgttgcccgt cgcgaagcga ttgaagaggc gggactgata
     4201 gtcaaacgga ccaaaccggt gttaagtttc ctggcaagcc cggggggcac cagtgagcgt
     4261 tcgtcaatta tggtgggcga agtggacgcc acgaccgcaa gcggtattca cggtctggct
     4321 gatgaaaacg aagatattcg cgttcatgtg gtaagccggg aacaggcata ccagtgggta
     4381 gaagagggga aaatcgacaa cgcagcgtcg gtcatcgctt tgcaatggct gcagctgcat
     4441 catcaagcgt taaaaaatga gtgggcataa atgaagcgtt acacacctga ctttcctgaa
     4501 atgatgcgcc tgtgcgagat gaacttttca caattgcgcc gtttgttacc gcgcaatgac
     4561 gcacccggcg aaactgtaag ctatcaggtg gcaaacgcac aatatcggct gacgattgtg
     4621 gaatcgaccc gatacactac cctggtgaca atagaacaga ctgcgcccgc gatcagttac
     4681 tggagccttc cgtcaatgac ggtgcgtctg tatcatgacg cgatggtggc tgaagtgtgt
     4741 tcaagccagc agatttttcg cttcaaagcg cggtatgatt atcctaataa aaagttgcat
     4801 caacgcgacg aaaagcatca aattaaccag tttttagcgg actggttgcg atactgttta
     4861 gcacatggag cgatggcgat tccggtttac tagcgtcgtg aaacctaagg acaccatttg
     4921 gaaagcctgt taacccttcc tctggctggt gaggccagag tcaggatttt acaaattacc
     4981 gacactcacc tgtttgcaca aaagcacgaa gccctgttag gggtaaacac ctgggaaagc
     5041 taccaggcgg tgctggaggc gattcgtcca caccagcacg aattcgacct gattgtcgcg
     5101 acaggtgatt tagcgcagga tcaatcctct gcggcctatc agcatttcgc tgaaggcatc
     5161 gcaagttttc gtgcgccctg cgtctggctg ccgggcaacc acgatttcca gcccgcgatg
     5221 tacagcgcgt tacaggatgc gggtatctcc ccggcgaagc gggtgtttat tggtgagcaa
     5281 tggcaaatcc tgttgctgga tagccaggtg tttggcgtgc cgcacggtga gctgagcgag
     5341 tttcagcttg agtggctgga acgtaaactg gccgatgcgc cagaacgcca tacgttgctg
     5401 ctgctgcatc atcatccgct acctgcgggt tgtagttggc ttgatcaaca cagcctgcgt
     5461 aacgcgggcg aactggatac cgtgctggcg aagtttccgc acgtcaaata cttgctgtgc
     5521 ggtcatattc atcaggagct ggatctcgac tggaacggtc gccgcctgct ggcaacgccg
     5581 tcgacctgtg tgcagtttaa gccgcactgt tccaacttta cgctggacac catcgcgccc
     5641 ggctggcgta ctctcgagtt acatgctgat ggcacgctga ccaccgaggt gcatcgcctg
     5701 gcggacacac gtttccaacc tgataccgct tcagaaggct actgatgtct acgcttcttt
     5761 atttacacgg tttcaacagc tcgccgcgct ctgcaaaagc gagcttgtta aaaaactggc
     5821 ttgcggaaca tcaccctgac gttgagatga tcatcccgca gttgccgccg tatccttccg
     5881 atgcggcaga actgctggaa tccattgtcc tggaacatgg cggcgattcg ctgggtattg
     5941 tcggttcgtc actgggggga tattacgcca cctggttgtc gcaatgtttt atgctgcccg
     6001 cagtggtggt aaacccggcg gtacgcccgt ttgaactgct gacggactat ctcggtcaga
     6061 acgagaaccc ctacaccggg cagcaatatg tgctagagtc acgccatatt tacgatctca
     6121 aagtcatgca gattgacccg ctggaagcgc cggatttgat ctggctgctg caacagacgg
     6181 gagatgaagt gctggattac cgccaggcgg tggcgtacta cgcttcctgc cgccagactg
     6241 tcatagaagg cggcaatcac gcattcacgg gcttcgaaga ttatttcaac ccgatcgtcg
     6301 attttcttgg tctgcaccat ctctgacgat caactcgaat tgctaactta aaccatgacg
     6361 caaacttata acgctgatgc cattgaggta ctcaccgggc ttgagccagt tcgccgccgt
     6421 ccggggatgt ataccgatac cactcgccct aaccatttgg ggcaagaagt tattgataac
     6481 agtgtcgatg aagcactggc gggtcacgca aaacgcgtgg acgtaatctt acatgctgac
     6541 cagtcgttag aagtgattga cgatgggcgc gggatgccgg tggatattca cccggaagag
     6601 ggtgtaccgg cggttgaact gattctttgc cgtctgcatg caggcggtaa attctctaac
     6661 aaaaattacc agttctctgg cggcctgcat ggcgtgggga tttcggtggt taacgccctg
     6721 tcgaagcgcg tagaagttaa cgtacgccgc gatggtcagg tttataacat cgcctttgaa
     6781 aatggcgaaa aggtgcagga tttacaggtt gtcggcactt gcggtaagcg caataccggt
     6841 accagcgtgc acttctggcc ggatgaaacc ttctttgaca gcccgcgttt ttctgtttca
     6901 cgcctgacgc atgtgctgaa ggccaaagca gtactgtgcc ctggtgttga gatcaccttt
     6961 aaagatgaga tcaacaacac cgaacagcgc tggtgctatc aggacggtct gaatgattac
     7021 ctggcggaag cggtaaacgg tttaccgacg ctgccagaaa aaccgtttat cggtaatttc
     7081 gctggcgata ctgaagcggt ggactgggcg ctactgtggc taccggaagg cggtgaactg
     7141 ctgaccgaaa gctacgtcaa cctgatccca acgatgcagg gcggtaccca tgttaatggc
     7201 ctgcgtcagg gcctgctgga cgcgatgcgt gaattctgtg aataccgcaa cattctgccg
     7261 cgcggtgtaa agctgtcggc ggaagatatc tgggatcgct gcgcctatgt gctgtcagta
     7321 aaaatgcagg atccgcagtt tgccgggcag accaaagaac gtctctcttc gcgtcagtgt
     7381 gcggcatttg tttctggcgt ggtgaaagat gccttcacct tgtggctgaa ccagaacgtt
     7441 caggcagctg aactgctggc ggagatggcg atttccagcg cccagcgtcg tatgcgtgcg
     7501 gctaaaaaag tggtgcgtaa aaagctaacc agcggcccgg cgctgcctgg taaattggct
     7561 gactgtaccg cgcaggatct taaccgtacc gaactattcc ttgtggaagg tgactccgca
     7621 ggcggatctg ccaagcaggc gcgcgatcgc gaatatcagg cgatcatgcc gctgaaaggt
     7681 aagatcctta acacctggga agtctcttcc gacgaagtgc tggcttcgca ggaagtgcac
     7741 gatatttcgg tagcgatcgg tatcgatcct gacagtgacg atctgagcca acttcgttac
     7801 ggcaagatct gtatcctggc ggatgctgac tccgatggtc tgcacattgc cacgctgctc
     7861 tgcgctttgt ttgtaaaaca cttccgcgca ttggtgaaac acggtcacgt ttacgttgca
     7921 ctgccaccgc tctaccgtat tgacctcggg aaagaggttt attacgcgct gacggaagaa
     7981 gagaaagagg gcgtacttga gcaattaaaa cgcaagaaag gcaagccaaa cgtccagcgt
     8041 tttaaaggtc tcggggaaat gaacccgatg caattgcgcg aaaccacgct tgatccgaac
     8101 actcgccgtc tggtgcagtt gactatcgat gatgaagacg atcagcgtac tgacgcgatg
     8161 atggatatgc tgctggcgaa gaaacgctcg gaagatcgcc gcaactggtt gcaagaaaaa
     8221 ggcgacatgg cggagattga ggtctgataa tcttaacccg gcaaacatgt gttttgccgg
     8281 gttgtcgttg attattttat tgggcacagt ttattacgat ctggaaatag tgttgccagc
     8341 gaaccagcca gagttttcca gtccgtagcc attttttctg cttgtgcagt tgttattact
     8401 gcaccttgtt gtttaataag ctcatcctcc actctggtat agtgaataat ggtaaaatcc
     8461 gtagaggacc atttatcctt agagaacatc ttcgtcatcg catgaatgtt tgcttgcccg
     8521 tcatcgataa aaacaatcgc gtcatatttg ttccccattt tatccagcat ataatcgaga
     8581 attacgcctt tatcttgccc ggaagaaaaa aataccccct ggctgtacag ccatgaccgt
     8641 ttgagtttcc ctttttcaaa gggtggcaaa gtatcctctt tcttcctgag tgcggaagac
     8701 gaaaaatcaa tgccgtttcg ctttaattcc cgcaatgtcg gaaagagagt atccggtgca
     8761 cgggatgtca gagcaaaagc ggtatgcttt tgttgccact gttttaacat tgtcggtaac
     8821 tgtggctcgg taagctgcat agggctaagt tcgtaaagca tactgattgt attttcatac
     8881 aggcaaggaa ctttttgcga gtcatccggc tttaaaggca atttatcggt ttgccattga
     8941 taccagatat caccgccaat gcgagtgcca ctcgttaata acgtgttatc aatgtcaaaa
     9001 accaataata tcttatcgga tggatagaga gtttcctttt ctgtgataat tttactgacc
     9061 gcactatatt cgccagtaac tatatttttt acctgtgcgt aagataaagc aggcagagca
     9121 gagagcatca tccagagaat gattttaaat ttattattca ttttttattc cagattagaa
     9181 agtataagtc agaccccagc gccagcggat ttggcgatta cttgatgttt tatccacacc
     9241 tttataatcc gcaatactca tatagggtat ccacgatccc attttgtacc ctactttaaa
     9301 tgatgcggta tagtttttat ggccatcatt ccaggttaaa taaccattat tgtaatagac
     9361 caggtttaac tgatattgga agttggtttt ttgtggtcga tagtcgatcc acacatcgta
     9421 acggcggtta ttacgatcat cgttgccata acctttggat ttatcggtgg ctttatccat
     9481 gtcataacga aaccgacctg aaactttcca ttctttgctg aagtcataac ccgcagaaag
     9541 accatattcg taagttgacc aatattgcgt ggtatcaagc cagaacatgg gttctaacca
     9601 gaattttttg tcatccagcg gcttaaagcg gtaaacaaca gataatcctg ttccgttatt
     9661 gccaaattgt gaggggtccc acttatcttt atcaccttca tcggtgttat agcgtaattc
     9721 tcccatcaaa ccgataccat tactaaaaaa tgtaccaaat tcaacgcggt ccgcgtgggt
     9781 gcgagtatca tcaagatact catggcgata atcaataaat ccatgcggct gttctgcaca
     9841 gactagtgta ggtga
//
LOCUS       DAZKGF010000178         9846 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZKGF010000178 DAZKGF010000000
VERSION     DAZKGF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.178"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..219
                     /locus_tag="ACHOQV_004208"
     CDS             <1..219
                     /locus_tag="ACHOQV_004208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632491.1"
                     /translation="WSWLATCLSGSRCQFLLTHRITVLCHFESHTSLPLTFYFSPPSS
                     TTFSPELTTLALTGKSYAGFLVTRVNQG"
     gene            252..764
                     /locus_tag="ACHOQV_004209"
     CDS             252..764
                     /locus_tag="ACHOQV_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9632492.1"
                     /translation="MKKTMLSACFLLVAGNAVANDGTIHFTGSITDQTCTVDSGSQNL
                     NVDLGNVGQTALNGAAGMKASPTRFTLNLSGCPDTVTGANVKFDGTSDGVNQNLLALD
                     SGTGIATGVGIEIADKNGAVIPLHTASMDYPLAAGSNTLDFVARYVSTGTAVTTGTAN
                     GTSQFTINYK"
     gene            878..1558
                     /locus_tag="ACHOQV_004210"
     CDS             878..1558
                     /locus_tag="ACHOQV_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9632493.1"
                     /translation="MNRYADVVLALLLAFLSVQAQAGIVIGGTRVIYDGDKKETAASV
                     RNPEKSGVYLVQSWVDSGEQGGKTPFIVTPPLFRINPGEENMLRIVRTGGNLPQDRES
                     VFWLNVKSIPATDDSHSRTNVLQVVVKSRLKLFYRPAGLEGQPESAYHQLSVAHSGNH
                     LTVSNPTPYYVTLFTLKVDGQEIKEADMVPPKGSVSFTLPSATASAVTWQAISDYGGV
                     SQTESRKL"
     gene            1570..4086
                     /locus_tag="ACHOQV_004211"
     CDS             1570..4086
                     /locus_tag="ACHOQV_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9632494.1"
                     /translation="MNNPTPRLMIILLTLLLAIVLLSGYLRNAEAREVFNPHALEIDN
                     PGQPVADLSTFAESGGQLPGTYRVTVFVNGEKQSDAQDIAFIDGAKKKLSPQITPAML
                     KAWGVKTDVFPALAALPPDQPLDDIGRYIPMANTELRFSKLQLNVSIPQAAMNASARG
                     YVDPSEWDEGVPAALLNYNLSGSNTWRDGQNGSDDNYYANLQSGINLGAWRLRNYSTW
                     NYDEDNGSHWDSVNTYLQRDIQRLKGQLTLGDSYTPSDIFDSVQFRGAQLASDDNMLP
                     DSLRGFAPVIRGIAQSNAQVTIKQNGYIIYQSYVAPGAFTITDLYPTSGSGDLEVTIK
                     EADGSERTFVQPFSAVPIMQREGRLKYALTAGKYRSGNEDSDEPQFGQVTAIYGLPHA
                     ITVYGGTLYSDDYQSGAAGLGFGLGELGSLSADITAAHTTLNNDETHNGQSYRVQYSK
                     DFQTTDTSFTLAAYRYSTAGFYTFQEANDLRADDDNGWQMTYNKRQRLQLDLSQSIGS
                     YGSFFISGYQQDYWQEDGYERTLSAGWNGNIDGISYSVTWSYSDYPESTQPADQQLAF
                     NIQVPLSRFMPNAWASYSVNTAKHGDTRQQVGLNGTALADNNLSYSLQQSYTNHGVGG
                     SGNINADYKGGQGEITGGYNYDDDTQQVNYGLKGGIVAHPHGITLSQPLGQSLAIVKA
                     PGADDTKVQNNTGVYTDWRGYAVVPYVNPYKKNRIALDTSTLGDEVDTDTAVQTVTPT
                     QGAVVMADFNTRVGRRVLMTLLYRGLPVPFGAEAKLKEGGSGIVGDDGQVYLTGVPEE
                     GDIAVNWNGAQQCVVHYRLPEQENQPSVILVNQECREVAK"
     gene            4083..5087
                     /locus_tag="ACHOQV_004212"
     CDS             4083..5087
                     /locus_tag="ACHOQV_004212"
                     /inference="COORDINATES: protein motif:HMM:NF012635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9632495.1"
                     /translation="MRVWRGMILLAGILFCQASWAFCTSSVSFSISPGTVVVQRDAVV
                     GEPISDWLVSNQGMAYQDCNYDATPQYVINAGIKNTGSRASVTWNGEAVFNSNVAGVG
                     FVFEGRSSVDSNPLPENWIGIASGQSQVFTLQISHSVGYHPSINDQARIRLIKTGDIT
                     PGTLSGNLGRFIAGTRENGQWSTEIPITFSGGQITTIACSVTTPDVNVPLGQHQKAEF
                     SGPGTGTGWQAFNIGLDCDKSARINVQIDAVQDPSNVAGVMKLDSAPGDMAATGIGVQ
                     LFFAPDNSAVQFGQSRYYYTSPNGGTETVQLKARYYQTAATITAGVANATATFTLTYK
                     "
     gene            5106..6053
                     /locus_tag="ACHOQV_004213"
     CDS             5106..6053
                     /locus_tag="ACHOQV_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9632496.1"
                     /translation="MRKILFFLLLMIAVLPEVQATCSFYGGVTSEVSGYLNFGNVVIQ
                     RDAQVGSTIATAITGAYNNGDGIAGCKREAWTARWELTQWGTLSGYGNGVYNTNLPGV
                     GLRLSAASTGKTLPYETSYAYNAGGTWVSIPGDGIKGELIKTGDITSGTLTDGVLARA
                     SVVNQFYFANVTLNGMNTVKSEACTVTTPSVDVPMGDHDKSEFSGAGSSTDWVSFDIG
                     LSCDVGARINVRIDATADASASSQGVMLIDSGGASGVGIQLHYRPDDAAVQYGQERFY
                     WQSVYGDEIVQLKARYYQTAGSITPGAANGTATFTMSYK"
     gene            6077..7612
                     /locus_tag="ACHOQV_004214"
     CDS             6077..7612
                     /locus_tag="ACHOQV_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9632497.1"
                     /translation="MSDIVAGNRIAAQYLRMSTEHQKYSPENQREYIEKYAKEHCITI
                     KYTYDDAGRSGLSLDNRPGLRSLLYDVENGIIKVSLILVYDVSRFGRFQDIEEAAHYS
                     WLLKRNGVRIIYCAEPFSEEQTELFMLGLSYSRYGAATYSKNLSQKVFLGQANLVRRG
                     YYQGGMPGYGIRRLLIDEHTNPKGMLLPGQRKSLQTDRVILIPGPAEEVVIVNKIYDM
                     FIDEGKPEFVIASELNRKNIPAENGLQWSRCKIHQILTNEKYIGNNVYNRTSGRLKTR
                     SVRNPQDQWIRCDGAWDPVVSVEKFEAAKRIIEARAVQYSDDELLDKLRHLLKLRGRL
                     SGIVIDEEELFPSSSIFRSRFGGLVRAYTLIGYHPRHDYSYLEIKKQIAECHLTFMDN
                     LVQDIYRYGGWAEMHQDGSLYINDEFFVSANLVRCQRLSSGKLRWKIKFNVSHYCDVM
                     IFPRLDSTNNSVVDYFMFPAIDVLYNDLTLHENNGFILELYRFDDLSCLENLVKRTGL
                     ESK"
     gene            7612..8499
                     /locus_tag="ACHOQV_004215"
     CDS             7612..8499
                     /locus_tag="ACHOQV_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP9632498.1"
                     /translation="MKSFDEQIELVDISDIYIANPRKRNKFIHNEIKENIKTIGLKRP
                     VSVRPVNNENYKYALICGQGRLEAYIEYGETKIPAIIKQVDEETAHLMSLAENIARRN
                     PRAAELMEDIRRMKTSGLTDREIGLRLGYNSSWVNGVITLLSSGEKHLLRAFETGDIP
                     LYLAVEISRSGDEETQELLTVALKEGHIKGGQINKIRRILEQRKKGDKGTISTAFFPG
                     KKQKKLTPEELANVYQENINEQKAILLKSTMVKEKILAVKQIFGYLLKDNDFVTLLHS
                     NNIHDIPSLLDGSDVSGEK"
     gene            8501..9391
                     /locus_tag="ACHOQV_004216"
     CDS             8501..9391
                     /locus_tag="ACHOQV_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning protein RepB C-terminal
                     domain-containing protein"
                     /protein_id="HFP9632499.1"
                     /translation="MISQCFDSRFYTFKVESLVYARTLPVNVKFSTKYKQIKASVDEI
                     GLIEPIVVYINDANDKVILDGHLRVEVLKELGITTAHCLISPVEDTYSYNKRVSRLTI
                     VQAQNMLLKAVESGVSVEKLCAVLGVTPDTLIGKLKISDGIAPEVMALLAEKNVPQTI
                     FSVLKKLKFYKQIEMVNTMISINNFTRKFAMSMLHAVAPEHLVAPKKDASRETDIRKN
                     LERLEKEMASVQVETQHLQDEYAENNLRLVIIKNHIERLLANGQVLNWLYDNHQEYLS
                     VLKQISGLDDLNKLGQTIEE"
     gene            9630..>9846
                     /locus_tag="ACHOQV_004217"
     CDS             9630..>9846
                     /locus_tag="ACHOQV_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632500.1"
                     /translation="MANQQAAKWAGVAAIAACLTFVVTTIGLLLAWRSLHQWKPQYKE
                     NSRLLLIEALIAFQKCLITIPKNLDNDH"
BASE COUNT         2522 a         2346 c         2692 g         2286 t
ORIGIN      
        1 tggtcctggc tggcaacgtg cttaagtggt tcacgttgcc agtttttact cactcaccgc
       61 attacggttt tatgccattt tgaaagccat acttcacttc cactgacatt ttatttttca
      121 cccccttcgt ccaccacatt ttctcctgaa ctcaccactc tggcactaac gggaaagtct
      181 tacgcgggtt ttctcgtcac cagagtgaat cagggttgag ccctgagtga tctttcatca
      241 aggagaaacg tatgaaaaaa acgatgttaa gcgcgtgttt tttactggtg gccgggaatg
      301 cggtcgccaa tgatggcacc atccatttta ccggcagtat taccgaccag acatgcaccg
      361 tggacagcgg ttcgcaaaac ctgaacgtcg atctgggcaa tgtagggcaa accgcactga
      421 acggtgcggc cgggatgaaa gcctccccga cgcgcttcac gctcaacctg agtggttgcc
      481 ctgataccgt taccggggcg aacgtgaagt ttgacgggac gtctgatggc gtaaaccaga
      541 acctgctggc gctggacagc ggtaccggga ttgcgaccgg cgttggtatc gaaatcgccg
      601 ataaaaatgg tgcagtcatt cctctgcata cggcctcaat ggattacccc ctcgcggcag
      661 gcagcaatac gctggacttc gtcgcccgct atgtttccac cggtaccgcg gtaaccacag
      721 gtacagctaa cggtacctcg cagttcacca ttaactacaa ataacgctgt gccgtcgcgg
      781 ggttcaccac ctctcccatc ctacccgacc tggtgtaccg ccgcataaca gggactgttt
      841 cgcagggatg cgtatttttc gtccggagaa caagaagatg aacaggtatg cagacgtggt
      901 gctggcactg ttgctggctt tcctgagcgt acaggcgcag gccgggatcg tgattggcgg
      961 tacgcgcgtg atttatgacg gggataaaaa agagacggcc gcctcggtac gcaacccgga
     1021 gaaatccggc gtctacctgg tgcaaagctg ggtagacagt ggcgaacagg ggggcaaaac
     1081 gccgtttatc gtcacgccac cgttgtttcg cattaatccc ggcgaagaga acatgctgcg
     1141 cattgtgcgc accggaggca acctgccgca ggacagggag tcggtgttct ggctgaacgt
     1201 gaaatcgatc cccgctaccg acgacagcca ttcgcgcacc aacgtgttgc aggtagtggt
     1261 caagtcgcgg ctgaaactgt tttaccgtcc ggcaggcctg gaagggcagc cggagagtgc
     1321 ctatcaccag ttgagcgttg cccacagcgg taaccatctt accgtcagca atcccacacc
     1381 gtattacgtc acgctgttca ccctgaaggt cgacgggcag gagattaaag aggcggatat
     1441 ggtgccgccg aaaggcagtg tgagtttcac gctaccgtca gcgactgcaa gcgcggtgac
     1501 ctggcaggcc atcagcgact atggcggtgt cagccagacc gaaagccgta agctgtgaag
     1561 gaggacgtga tgaataaccc gacgccacgt ctgatgataa tcctgctgac gctgttgttg
     1621 gcaatagtgc ttttgtctgg ctatttgcgt aatgccgagg cgcgtgaggt gtttaacccc
     1681 cacgcgctgg agattgataa ccccggccag ccggtggcgg atttatccac ctttgcggaa
     1741 tccggtggtc agctacctgg tacctaccgc gtaacggtct tcgtgaacgg tgaaaagcag
     1801 agcgacgcac aggatattgc ttttattgac ggagccaaaa agaaactcag cccgcagata
     1861 acgcctgcga tgctgaaagc ctggggcgtc aaaacggacg tcttcccggc actggcagca
     1921 ctgccgccgg atcaaccact ggatgatatt ggccgctata tcccgatggc caacactgaa
     1981 ctgcgcttca gtaaattgca gttgaacgtc agtattcccc aggcggcaat gaatgccagc
     2041 gccagaggat acgtggatcc gtccgaatgg gacgaaggtg tccccgccgc actgctgaac
     2101 tacaacctca gcggcagcaa cacctggcgc gatggacaaa acggttcgga tgataactac
     2161 tacgcgaacc tgcaaagcgg tatcaacctc ggcgcgtggc ggctacgcaa ctactcgacc
     2221 tggaattatg acgaagataa tggtagccac tgggattcag taaacacgta cctgcaacgg
     2281 gatatccagc gcctgaaggg acaactgacg ctcggcgaca gctacacgcc gtcggacatc
     2341 ttcgacagcg tccagtttcg cggcgcgcag ttggcatctg acgacaacat gctgccggac
     2401 agcctgcgcg ggttcgcccc ggtgatccgc ggtatcgccc agtctaacgc tcaggtgacc
     2461 attaagcaga acggctacat catctaccag agctacgtcg cgccgggtgc gtttaccatc
     2521 accgatctgt atccgacctc gggcagcggt gacctggaag tgaccatcaa agaggccgac
     2581 ggcagcgagc gcaccttcgt ccagccgttc tcggcggtgc cgattatgca acgtgaaggc
     2641 cgcctgaaat atgccctgac cgccgggaaa taccgcagcg gcaatgagga cagcgacgag
     2701 ccgcagttcg gccaggtcac tgcgatttac ggtctgccgc atgctatcac cgtctatggc
     2761 ggcacgctgt actcggacga ttatcagtct ggcgccgcag ggctcggttt tggcctcggt
     2821 gaactcggtt cgctgtcagc cgatatcacc gccgcccata ccacgctcaa taacgatgaa
     2881 acccacaacg ggcagtcgta ccgggtgcag tattcgaagg attttcagac caccgacacc
     2941 agcttcacgc tggcggcata tcgctactcc accgccggat tttacacctt ccaggaagcc
     3001 aacgacctga gggctgatga tgataacggc tggcagatga cgtacaacaa acgccagcgc
     3061 ctccagttag atctcagtca gagtatcggt agctatggct cgttttttat ctccggctac
     3121 cagcaggatt actggcagga ggacggctac gaacgcacgt tgagcgcggg ctggaacggg
     3181 aatatcgacg gtatcagcta cagcgttacc tggagttaca gcgactaccc ggagagcacg
     3241 cagcctgccg accagcaact ggcgttcaac atccaggtgc cgctcagccg ctttatgccg
     3301 aacgcctggg cgagctacag cgtcaacacc gcgaaacacg gcgatacccg ccagcaggta
     3361 gggctgaacg gtacggcgct tgccgacaac aacctgagct acagcctcca gcagagctac
     3421 accaaccatg gtgtgggcgg ctccggcaat atcaacgccg actataaagg cgggcagggg
     3481 gaaatcaccg gaggctacaa ctatgacgac gacacgcagc aggttaacta cgggctgaag
     3541 ggcggtattg tcgcgcatcc gcatggcatc acgctttcgc agccgctcgg ccagtcgctg
     3601 gcgatcgtga aagcgcccgg tgcggatgat acgaaggtac agaacaacac cggggtttac
     3661 accgactggc gcggttatgc ggtagtgccg tatgtgaacc cgtacaagaa aaaccgtatt
     3721 gcgctcgaca ccagcacgct tggcgacgaa gtggataccg acactgccgt acagaccgtg
     3781 acccccacgc agggtgccgt ggtgatggcg gattttaaca cccgtgtcgg gcggcgtgtg
     3841 ctcatgacgc tgctgtaccg tgggctgcca gtcccctttg gcgcagaggc gaagctgaaa
     3901 gagggcggca gcggtattgt cggcgatgat ggccaggtct atctgactgg cgtgccggag
     3961 gaaggcgata ttgcggttaa ctggaacgga gcacaacagt gcgtggtgca ttaccgttta
     4021 ccggaacagg aaaaccagcc gtcagtgatc ctggtgaacc aggaatgcag ggaggtggca
     4081 aaatgagagt atggcgcggg atgattctgc tggcggggat cctgttctgc caggcgtcgt
     4141 gggcgttttg cacgtcttcg gtgagtttct ccatcagccc cggtacggtg gtggtccagc
     4201 gggatgctgt ggtgggggaa cccattagcg actggctggt gtcgaaccag gggatggcct
     4261 atcaggactg caattatgat gcaacgccac aatacgtcat caatgcaggc atcaaaaaca
     4321 cgggctcccg cgccagcgtc acctggaatg gtgaggcggt gtttaacagt aatgtcgcag
     4381 gtgtgggatt tgtgtttgaa ggccgctcct cggtcgacag caatccgtta ccggaaaact
     4441 ggatcggtat cgccagcggg cagtcgcagg tattcacgct ccagataagc cacagcgttg
     4501 gctaccatcc ctccattaac gatcaggcgc ggatccggct gataaaaacc ggcgacatta
     4561 cgcccggtac gctgagcggc aacctggggc gttttatcgc ggggacaagg gagaacgggc
     4621 agtggtcgac tgaaattccc attacatttt ccggtgggca aatcaccacc atcgcctgct
     4681 cagtgaccac gcctgatgtc aatgtgccgc tggggcagca tcaaaaagcg gagttctccg
     4741 ggccgggaac gggaaccggc tggcaggcgt ttaacatcgg gctggactgc gataaaagtg
     4801 cccgtatcaa cgttcagata gatgccgtac aggatccgtc caacgtggct ggcgtgatga
     4861 agctggacag cgcgcccgga gacatggcgg ctacgggcat cggtgtccag ttgttctttg
     4921 caccggataa cagcgccgta cagtttggtc agtcacgcta ttactatacc tctcccaatg
     4981 gcggtactga gacagtgcag cttaaggcgc ggtattacca gaccgcagcc acgattactg
     5041 caggtgttgc caatgccacg gcgaccttca cgctgaccta taaatgacgt cgccggagtg
     5101 ttgagatgag aaaaattctt ttcttcctgc tgttgatgat agccgtcctg ccggaggttc
     5161 aggccacctg ttcgttctac ggtggtgtga catcagaagt atcgggttac ctgaatttcg
     5221 gcaacgtggt tatccagcgg gatgcacagg tcggcagcac gattgccact gccattacag
     5281 gggcatacaa caatggcgac ggcattgccg ggtgtaagcg ggaggcctgg accgcccgct
     5341 gggaactgac gcaatggggg acgctgagtg gctatgggaa tggtgtctac aacaccaacc
     5401 tgccaggggt aggattacgt ctgagtgcgg cctcgacagg taaaacattg ccctatgaaa
     5461 ccagctacgc ctacaacgct ggcggcacct gggtatccat tcccggtgat ggcatcaagg
     5521 gcgagcttat caaaaccggg gatatcacca gcggtacatt aacggacggt gtgctggcgc
     5581 gcgcttcggt agtgaatcag ttttattttg ccaacgtcac gttgaacggg atgaacacag
     5641 tgaaatcaga agcttgtact gtgaccacgc cctctgttga tgtcccgatg ggggatcacg
     5701 ataaaagcga attcagtggt gcgggatctt caaccgactg ggtgagcttt gatattggtt
     5761 tgagctgcga tgtcggggcg agaataaacg tacgtatcga tgccactgcg gatgcttccg
     5821 ccagttcgca aggcgtcatg ctgattgata gcggcggggc gtctggcgtg gggatacaac
     5881 ttcattaccg cccggatgat gccgctgtcc agtacggtca ggaacggttt tactggcagt
     5941 cggtatatgg tgacgagatc gtgcagctta aggcgcgata ttaccagact gcaggctcga
     6001 tcacgccagg tgcggcaaac ggtacggcga cgtttacgat gagttataaa tagcgcgtaa
     6061 tcatatggaa tattctatgt cggatattgt cgccgggaac aggattgctg ctcaatattt
     6121 aagaatgtct accgagcatc agaaatattc gccggagaac cagcgggaat atatcgagaa
     6181 gtatgcaaaa gaacactgta ttaccattaa atatacctat gacgacgctg gccgaagtgg
     6241 gctgagcctg gataacaggc ctggcctgcg ttcgctgtta tatgatgttg aaaacggaat
     6301 aataaaagtc tcgttaattc ttgtttacga tgtcagtcgg tttggtcgtt ttcaggatat
     6361 tgaagaggct gctcattact cgtggctact gaagcgtaat ggtgtgcgca ttatttattg
     6421 cgcggagcct ttctcggaag aacagaccga attgtttatg ctgggattat cttactcccg
     6481 ctatggcgcc gcaacttaca gtaaaaacct ttcacaaaaa gtttttcttg gccaggcaaa
     6541 cctcgttcgg cggggttatt atcaaggtgg tatgcctggc tatggcattc ggagattatt
     6601 aattgatgaa catactaatc cgaaaggcat gcttctgccg gggcaaagga aaagcctgca
     6661 aactgacaga gtaatattga tcccggggcc cgcggaagag gtggtaatag tcaataaaat
     6721 ctatgacatg tttattgatg agggcaagcc tgagtttgtt attgcttcag aactgaaccg
     6781 gaagaacatc cccgccgaga atgggctcca gtggagccga tgtaaaattc atcagatact
     6841 gaccaatgaa aaatatattg gcaataatgt ttataacaga acctccggac gattgaagac
     6901 ccgaagtgtc aggaatcctc aggatcagtg gatcagatgt gacggcgcgt gggaccctgt
     6961 ggtatcagta gagaaatttg aggcggctaa aaggattatt gaagccaggg ccgttcagta
     7021 cagtgatgat gaactgctgg ataaactacg ccatctgctg aagcttcgtg gcagattatc
     7081 cgggattgtg attgatgaag aggaactgtt tccatcgagc agcatttttc gttcacggtt
     7141 tggtgggtta gtcagggctt acacccttat tggttatcac ccgcgccatg attactctta
     7201 tcttgaaata aaaaagcaga ttgcagaatg ccatctcact ttcatggata atttggtgca
     7261 ggatatatac cgatatggcg gttgggcaga gatgcatcag gatggttcat tatatattaa
     7321 tgatgagttt tttgtttccg ctaatctggt tcgctgccaa cgtttgtcgt caggtaagct
     7381 tcgttggaag ataaaattta atgtatccca ttattgtgat gtcatgatct ttccacggtt
     7441 ggatagcaca aataactccg tggttgatta ctttatgttt ccggctattg atgtgttgta
     7501 taacgattta acgcttcatg agaacaatgg ttttatcctc gaactatatc gatttgatga
     7561 tttgtcatgt ctggaaaatc ttgtaaaacg aactggtctg gagagtaaat aatgaaatct
     7621 tttgatgaac agatagaact tgtcgatatc agtgatattt atatcgcaaa tccacggaaa
     7681 aggaataaat tcattcataa tgaaataaaa gagaacataa aaaccattgg tctgaaacgg
     7741 cctgttagcg tgcgcccagt gaataatgaa aactataaat atgccctgat ttgtggtcag
     7801 ggacgacttg aagcgtatat tgagtatggt gaaacgaaaa ttcctgcaat catcaagcag
     7861 gttgatgaag agacagcgca cctgatgagc ctggctgaaa acatcgcaag acgtaatccc
     7921 cgggcggcag agctaatgga agatatcaga agaatgaaga ccagtggcct gaccgatcgt
     7981 gaaataggct tgcggcttgg gtataattcg agttgggtaa atggggtgat cactctgttg
     8041 tcatccggag agaagcatct tttgcgggca tttgagactg gcgatattcc cttgtatctt
     8101 gctgtggaaa tatcccggtc aggagatgag gaaactcagg aactgctgac ggtcgccctg
     8161 aaagaaggac atatcaaggg tgggcaaatt aataagatca ggcgcatact tgaacaacgt
     8221 aagaaaggcg ataaaggtac gattagtacg gctttttttc ctgggaaaaa acagaaaaaa
     8281 ctgaccccgg aagaactggc taatgtctac caggaaaaca ttaatgagca aaaagccatt
     8341 ctgctgaaat ccacaatggt taaagagaaa attctggcag taaagcaaat atttggttat
     8401 ttacttaaag ataatgattt tgttacattg ctgcacagta ataatatcca cgatattcct
     8461 tctttactgg atggcagtga tgtatcgggg gagaaataag atgatttcac aatgttttga
     8521 ctcccgattt tataccttta aggtggaatc actggtgtat gccagaacgc tgccggttaa
     8581 tgttaaattc agtacgaagt acaaacaaat taaagcctct gtcgatgaaa ttgggttaat
     8641 tgaacccatt gtggtttata tcaatgacgc caatgataaa gtcattctgg atggacatct
     8701 tcgcgttgaa gttttgaaag aactcggaat aactactgct cattgtctga tatcccctgt
     8761 ggaggataca tattcatata acaaacgtgt tagtcgcctt accattgtgc aggcccaaaa
     8821 catgctgctt aaagcggttg aatctggcgt ttcagttgaa aaactatgcg ccgtactggg
     8881 tgtcacgcca gatacgctga ttggtaaact gaaaatttct gatggcattg cccctgaagt
     8941 catggcgtta ctggcagaaa aaaatgtacc gcagaccatt ttctctgtgt tgaaaaaact
     9001 aaagttctat aagcagatag aaatggttaa caccatgatc agtattaata atttcacccg
     9061 caaatttgcg atgtcgatgc tgcatgccgt tgcccctgaa catcttgtag cgcctaaaaa
     9121 agatgccagc agagaaacag atatcagaaa aaatctcgaa agactggaaa aagaaatggc
     9181 ttccgttcag gtggaaacgc agcatcttca ggatgaatat gctgagaata accttcggct
     9241 ggttatcatt aaaaatcaca ttgaacgtct gctggcgaac ggtcaggtac ttaactggct
     9301 ttatgataac catcaggagt acctttctgt gctcaagcaa atctcggggc tggatgattt
     9361 gaataagctt ggccagacaa ttgaggaatg acacattttt aaagcaagga ctgctgtttt
     9421 acagattgcc attatgatgg gatgcactga gagaggaggg cgtgctggtg gttgacgtga
     9481 ttcaactggt gcggaaaatc ttgctcaaag gacgatggct taaaatcaag ttgctgtttc
     9541 aaaaatgaaa aagtagattt acatccactt ttcttactga ggtaaaaata gtgcatatag
     9601 atgcggattt cataagcttg agcacattgg tggctaatca acaagctgca aaatgggcgg
     9661 gggttgctgc tattgctgcc tgtttgacgt ttgttgtgac aacgataggt ttactacttg
     9721 cttggcggtc gttgcatcag tggaagcctc aatacaaaga aaactctaga cttctcctaa
     9781 tcgaagcttt aatcgcattt cagaaatgtt taataactat accaaaaaat ctagataacg
     9841 atcacc
//
LOCUS       DAZKGF010000179         9723 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZKGF010000179 DAZKGF010000000
VERSION     DAZKGF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.179"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            270..632
                     /locus_tag="ACHOQV_004218"
     CDS             270..632
                     /locus_tag="ACHOQV_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632501.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMGKGREE"
     gene            complement(735..1193)
                     /gene="ybbJ"
                     /locus_tag="ACHOQV_004219"
     CDS             complement(735..1193)
                     /gene="ybbJ"
                     /locus_tag="ACHOQV_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP9632502.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLSAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1190..2107)
                     /gene="qmcA"
                     /locus_tag="ACHOQV_004220"
     CDS             complement(1190..2107)
                     /gene="qmcA"
                     /locus_tag="ACHOQV_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9632503.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2253..2930
                     /gene="fetA"
                     /locus_tag="ACHOQV_004221"
     CDS             2253..2930
                     /gene="fetA"
                     /locus_tag="ACHOQV_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFP9632504.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDTILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2917..3696
                     /gene="fetB"
                     /locus_tag="ACHOQV_004222"
     CDS             2917..3696
                     /gene="fetB"
                     /locus_tag="ACHOQV_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFP9632505.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3759..4613)
                     /gene="cnoX"
                     /locus_tag="ACHOQV_004223"
     CDS             complement(3759..4613)
                     /gene="cnoX"
                     /locus_tag="ACHOQV_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFP9632506.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4674..5483)
                     /gene="ybbO"
                     /locus_tag="ACHOQV_004224"
     CDS             complement(4674..5483)
                     /gene="ybbO"
                     /locus_tag="ACHOQV_004224"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP9632507.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHCGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5473..6096)
                     /gene="tesA"
                     /locus_tag="ACHOQV_004225"
     CDS             complement(5473..6096)
                     /gene="tesA"
                     /locus_tag="ACHOQV_004225"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFP9632508.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            6067..6753
                     /gene="ybbA"
                     /locus_tag="ACHOQV_004226"
     CDS             6067..6753
                     /gene="ybbA"
                     /locus_tag="ACHOQV_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFP9632509.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6750..9164
                     /gene="ybbP"
                     /locus_tag="ACHOQV_004227"
     CDS             6750..9164
                     /gene="ybbP"
                     /locus_tag="ACHOQV_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFP9632510.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2271 a         2427 c         2636 g         2389 t
ORIGIN      
        1 ctacgccgcc atttgtgtga tgtaaagcgc atttttgtct ggtgtaagcg taaacaccac
       61 caataagaaa caaaaaatgt ttcttatata aataaaatcg aaaatcattt tgtgctcttt
      121 caatgtaaaa aagagtaaag acttgttttg ataagcgaaa taaattctgt ttctggacta
      181 aacgtgaagc ggttcctgat gtgtgctaaa atgcgtttct ttgataaatc tcaattttta
      241 agaggtttac gccaggcagg aaaatttaaa tgaatgaatt tgagaagatt tttaatgaaa
      301 tgaatctgga ccgggcattg ttaccgatcc tttttcgttc gaaccggtcg acagtgtgga
      361 agtatttaag cggcgatagt acggctcctg cctctgccat gtcgctgatc atgctgttgc
      421 agttgatcca gaaacgaaac cccgatctgt tagccgaatg gttaacgctt tctgatttca
      481 ccattccgcc agaagtttac ctggaccaac ctgattactg gaaagggtgg gtgtataccc
      541 aacacaaggt caataaaaac gtcctggaat atctaaaaaa acactatccg gatgaagacc
      601 aaaaaagtat gggtaaaggc cgggaagagt aattttgcct ctactcggct atatgccgga
      661 tgcggcgcaa gcgccttatc tggcctacga ttcgggcata ttttgtaggc atgataagac
      721 gcgccagcgt cgcatcaggc gatgaccgca cggataatca gcgttatccc ttctatcgca
      781 atcacctcaa catgcgtacc tgcactgaga tcctcgctgg cgctgacagg ccatgaactg
      841 tcaccgacgc gcatatgacc acgcccgttg accagcggag attccagaac aaaacgtcgg
      901 ccaatcaact gctgcccgcg ctggtttaaa tgactgtcgc tgtgcttttg ttcgcgcacc
      961 cgccgcgaca accatttcca ccacagccag gcggcgagca gcgtcaggac ggcaaacatt
     1021 accccttgcc actcccaacc cagcggcagc aaccagacca ccagaccggt aatcaccgct
     1081 gccacgccgc tccacaacag ataaccattg ccgcccaaca tctcggctgc cagcagcaaa
     1141 ccgccgagac ttagccagaa aatatgtgga tgaacgacca ttaactccat catggctgag
     1201 tccgtttgtt ggcgctgtct ttcaccagct cagcaatccc ggcaatcgac cccatcaggc
     1261 tgctggcctc taatggcatc atcaccactt tgctgttact ggacgaaccg atctgctgta
     1321 acgcttcggt gtatttctgt gcgacgaagt agttcaccgc ctgaatatca ccggaggcaa
     1381 tggcttcaga caccatttta gtggcgcggg cttctgcttc agcggaacgt tcacgcgctt
     1441 cagcctgtaa aaacgccgac tgacgttcgc cttccgcttt caggatttgc gactgttttt
     1501 caccttcggc tttgaggatt tccgcctgac ggatcccttc cgcttcaagg atgtaagcgc
     1561 gtttggtacg ttccgctttc atctgcgcgt tcattgaaga gataagctct gccggtgggc
     1621 gcacgtcgcg aatttcaata cgggtgactt taatccccca cgggttggtg gcctcatcga
     1681 caatacgcag caggcgtgag ttgatgctgt cgcgctgaga gagcatttcg tcaagttcca
     1741 ttgaacccag cacggtacgg atgttagtca tggtcaggtt gatgatcgcc agctccagat
     1801 tgctgacttc ataagccgcg cgcggcgcgt caatcacctg aataaagcac acggcgtcga
     1861 tggtaacgtt ggcgttatct ttcgagataa cttcctggga agggatatcg agcacttgct
     1921 ccatcatatt gatcttgcga ccaatgcgat ccataaacgg caccaccaga ctgagccccg
     1981 gctgtaacgt tttggtatag cgaccaaaac gttctactgt ccactgatag ccctgcggca
     2041 cgattttgac gcccgcgccg acaatgacca gcgcgacaaa aatgagaatc gggataaaga
     2101 taagcatcgg aaaaacctcc tgttgtaccg tccataatca gcaaaattgc tgcttgatta
     2161 aacaaattat acctgattac tgaaagagag ttccccctta ttcctgcgaa ggataaactg
     2221 tttttagtaa aaatcagaaa aagggaacag tgatgcagga aaatagtcct ttgcttcagc
     2281 tacaaaacgt aggatatctg gcgggtgatg cgaagattct taataacatc aatttttcgc
     2341 tgcgtgctgg cgaatttaag ttaattaccg gtccttctgg ttgtggcaaa agtacgctgc
     2401 taaaaatagt tgcttcattg atcagcccaa ccagcggaac gttactgttt gaaggtgagg
     2461 atgtcagcac actaaagcca gaaatctacc gccagcaagt ctcttattgc gcccagacgc
     2521 cgacgctgtt tggcgacacg gtatacgata atctgatctt tccctggcag atccgtaacc
     2581 agcagcctga cccagccatt tttctcgatt ttctcgaacg cttcgcctta ccggacacca
     2641 ttttgacgaa gaatatcgcc gagctatctg gtggtgaaaa acaacgcatc tcattgattc
     2701 gtaacctgca atttatgccg aaggttttat tgctggatga aataaccagt gcgctggacg
     2761 aaagtaataa acataacgtt aatgagatga tccaccgtta tgtgcgcgag caaaatattg
     2821 ccgtgctgtg ggtgacacac gataaagacg aaattaatca tgcggataaa gtgattacac
     2881 tgcaaccgca tgccggagaa atgcaggaag cacgctatga actcgcataa tattactaac
     2941 gaatcattag cactggcatt gatgctggtg gtggtggcaa tcttaattag ccataaagaa
     3001 aaactggcgc tggagaaaga tattctctgg agcgtcgggc gcgcgataat ccagctgatt
     3061 attgtcggct atgtgctgaa gtatattttc agcgtggatg atgccagcct gacattattg
     3121 atggtgttat ttatctgctt taatgcggcg tggaacgcgc aaaaacgcag taaatatatt
     3181 gctaaagctt ttatctcatc gtttattgcc attacggtcg gggcgggaat taccctggcg
     3241 gtgctgattc tctcagggtc gattgaattt atcccgatgc aggtgatccc tatcgccggg
     3301 atgatagccg gcaacgccat ggtagcggtg gggttgtgtt acaacaattt agggcaacgg
     3361 gtcattagcg aacagcaaca gattcaggag aaactgagtc ttggcgcgac gccgaaacag
     3421 gcttcagcga tattgattcg cgacagtatt cgcgcggctt taattccgac ggtcgattca
     3481 gcaaaaacgg taggcttagt gagtttacca ggaatgatgt ccgggctgat atttgccggg
     3541 attgatccgg tgaaggcgat taaatatcag attatggtga cctttatgct gctctcaacc
     3601 gccagcttgt cgaccattat tgcctgctat ttaacctatc gtaagtttta caattcgcgc
     3661 caccaattgg tggtgacgca attgaagaag aaatgatgcg atgccggacg cgcagcatcg
     3721 catccggcgt tgtggttcat gtgccgatca acggaaactc aatacaacaa cgcgtacagc
     3781 tggcggcgat acttcgacgc cagtgcatca cccgtaccca gcgcagcgag gatctcctgg
     3841 aatgttttac gtgtctgacc atctgcggcg gtgagatctt tacgcagatg cccaaacagt
     3901 aactccagcg cctcttcatt gcgcccaacc tggtgcagtt gcagtgccag ttgtgtcgcc
     3961 agtgcggcat cttctggatt ctccgccacc tgctgttgca actgttgaat ttccggcgta
     4021 tcagccgcct gcttcagcag ttcgatttgc gccaccagcc cctgatagcg ggtgtcctga
     4081 tcctgcaacg gaatggtttt cagcacggct tctgcatctt cagaacggtt cagcgcaatc
     4141 agcgtttctg ccagcagcag gccgatctcc ccgttctgat tcgacaactg ccaggcgtct
     4201 ttcagcaatg gcaaggcgtc ggtgtaattg ccttcctgca tcagctgcat cgcctgctgc
     4261 gctttcagct cttcttcgcg cggcagcact ttatccagca gggcgcggat cgcctcttcc
     4321 ggttgcggcc cctggaagcc atctaccggt tgcccgttct ggaacagata cacggtcgga
     4381 atcgcgcgca gaccaaactg ggcggcaatc atctgctccg cgtcgcagtc cagcttcgcc
     4441 agaataaatt gcccgttgta ctgcgccgcg aggctttcca ggatcggggt taactgcaaa
     4501 cagtgctggc tacgttcaga ccaaaaatag aacagcaccg gagtggtcat cgactgttca
     4561 agaacctgtt gcaggttaga ttcgttaatg ttgacaatat tttctacgga catggagtcg
     4621 ctctctgttg tcgatatttt ctttgacatg ggggcttaag cgcgcgcttc aactcacccc
     4681 tgcaatattt tgtccatcac gcgccccggc agcaggcgct taagcaccat taccgcccag
     4741 gtcaccagcg ttaccggata gcgcatcttc ggcttctcgc taataaaagc atggcgtact
     4801 ttgtccacca ccgcttccgg tcccaacgta aagcgggcgg cgatgccggg attttcgact
     4861 ggtttatcac tttgcgtctg gttgacgttg tcggtgaagc gagtacgaat gggaccgggt
     4921 tcgatcaggc tgactttaat tccacagtgg cgtagctcca tgcgcagcgc gtctgaccac
     4981 gcctccagcg catatttact ggccgcgtaa gcaccacgac ccggcgtgga gattaatccc
     5041 atcaccgatg aggtcatcac aatacgccct tcaccgtgcg gtaacatcgc gggtaacagg
     5101 cgcatggtga gctggtgtgc gccgaaaaag ttggcggaaa actgctgttc catctgcgca
     5161 cggctgatgg tggaaagggg gccatacatg ccgaatccgg cattgttaaa gatcccatac
     5221 agacaattat cggtcagggc gatcacctcg tcggctgcgc gatcaacact ttctggtgaa
     5281 tccagatcga tcaacacgcc ggtaaatccc atgctgttca tgcgctcaac atcatccggt
     5341 ttccggcaac ctgccagcac atgaaaaccc tggcgtttta attcgagcgc gctttccagg
     5401 ccaattccac tggaacatcc ggtaattaag accgattttt gcataacttt acctgtcagg
     5461 atctccgttg ctttatgagt catgatttac taaaggctgc aactgcttcg ccatccagtc
     5521 ggcaataaac ggctgggcgt cgcggttggg atgaataccg tcatcctgca tccattgtgg
     5581 tttgaggtag acctcttcca taaaaaaggg cagcagcgga acatcaaact ctttggcgag
     5641 tttggggtaa atggcgctaa aggcttcatt ataacggcga ccatagtttg caggcagacg
     5701 tatttgcatt aacaatggtt cagcgttggc ggctttgaca tcctgcaata tctggcgcag
     5761 cgtttgctcg gtttgttgtg gctgaaaacc acgcaaaccg tcattgccgc ccagttctac
     5821 cagcacccag cgcggctgat gctgtttcag cagagccgga aggcgcgcca gcccttgttg
     5881 cgaggtgtcg ccgctgatgc tggcattgac taccgacgtt ttactctgcc acttatcgtt
     5941 caacaaggca ggccaggccg cgctggcaga cattcgatac ccggcgctca ggctatcacc
     6001 cagaatcaat aacgtgtccg ctgcggcggc acggaaggtt aacaggacca ggaacaggaa
     6061 gggcaaatgc cagcggaaaa cattgttgaa gttcatcatc ttaagaagtc cgtcggtcag
     6121 ggggagcatg aactctccat cctcaccgga gttgagctgg ttgtcaaacg tggcgagacc
     6181 atcgcactgg tgggcgagtc gggatcgggt aagtcaactt tgctggcgat cctcgccggg
     6241 ctggatgacg gcagcagtgg cgaagtgagt ctggtgggac aaccgctaca taatatggac
     6301 gaagaagcgc gggcaaagtt gcgcgcgaag cacgtcggct ttgtttttca gtcatttatg
     6361 ttaattccta cccttaacgc gctggaaaac gtcgagcttc cggcgctgct gcgcggtgag
     6421 agtagcgcgg aaagtcgtaa cggggcgaaa gcgttgctcg aacagttagg gctgggtaaa
     6481 cgtctggatc atcttccggc acagctttcc ggcggtgaac agcaacgagt ggcgctggca
     6541 cgagccttta acggtcgacc tgatgtgctg tttgccgacg aacccaccgg caatcttgac
     6601 cgacagacgg gcgataaaat tgccgacctg ctattttccc tcaaccgtga gcatggcacc
     6661 acgttgatta tggtgaccca cgacctgcaa ctggcggcac gctgcgaccg ctgcttacgg
     6721 ctggtgaacg ggcaattgca ggaggaagca tgattgcacg ttggttctgg cgcgaatggc
     6781 gctcgccgtc gctattaatt gtctggctgg cgctaagcct ggcggtggcc tgcgtgctgg
     6841 cgctgggcaa tatcagcgat cgcatggaga agggcttaag ccagcaaagc cgggagttta
     6901 tggcgggcga tcgggcgttg cgcagttcac gcgaagtgcc acaagcgtgg ctggaggaag
     6961 cgcaaaagcg cggcctgaaa gtcggcaagc agctgacttt cgccacaatg acctttgcag
     7021 gcgacacacc gcagctggcg aacgtcaaag cggtggatga tatctacccg atgtatggcg
     7081 atctgcaaac taatccccct ggcctgaaac cgcaggcggg cagcgtattg ctggccccac
     7141 gcctgatggc actgcttaac ctgaaaacag gcgacaccat cgacgtgggt gatgccacct
     7201 tgcggattgc cggagaagtg attcaggaac cggattccgg ttttaacccc ttccagatgg
     7261 ctccgcgtct gatgatgaat ctggcggatg tcgataaaac cggagccgtg cagccgggga
     7321 gtcgggtcac ctggcgctat aaattcggcg gcagcgagaa ccagctcgac ggctatgaga
     7381 aatggttgtt accccagctt aaacctgaac aacgctggta cggtctggaa caggacgaag
     7441 gcgcgctggg gcgatcgata gaacgttcgc aacagttcct gctgctttcg gcgcttctga
     7501 ccttgctgct ggcggtggcg gcggttgcgg tggcgatgaa tcattactgt cgcagtcgct
     7561 acgacttggt ggcgatcctc aagacgctgg gggcagggcg agcgcaactg cgtaagttaa
     7621 tcgtcggtca gtggttgatg gtgctggcgc tttcagccgt taccggtggt gcgataggcc
     7681 tgttgttcga aaacgtgttg atggtgctgc tcaagcccgt tttgcctgct gcactaccgc
     7741 cagccagcct ctggccgtgg ttgtgggcgc tgggcaccat gacggtcatc tcgctgctgg
     7801 tggggctacg accgtaccgc ttgttgctgg caacgcagcc tttacgcgta ttacgtaatg
     7861 atgtggtcgc gaacgtctgg ccgctgaagt tttatctgcc gattgtcagt gtggtggttg
     7921 tgctgctgct cgccggatta atgggtggca gcatgctgct ttgggcggtg ctggcgggcg
     7981 cggtagtact ggctttgctg tgcggtgtgc tgggctggat gctgctgaat gtacttcgcc
     8041 gcatgacgct gaaatcgcta cctctgcgcc tggcggttag ccgcctgtta cgtcagccgt
     8101 ggtcaacgtt aagccagctt tcggcatttt cgctctcctt tatgctgctg gcgctgttgc
     8161 tggtattgcg tggcgatctg ctcgaccgct ggcagcagca gctacctcca gaaagcccga
     8221 actacttttt aattaacatc gccacagaac aggtagcacc gctaaaagcg ttcctcgcgg
     8281 aacatcagat agtcccggaa tcgttttatc cggtggtgcg ggcgcggctg acggcgatta
     8341 acgataagcc gacagaaggc aatgaagatg aggcgctcaa tcgcgaactc aatcttacct
     8401 ggcaaaatac gcggcccgat cataatccga ttgtcgccgg taactggccg ccaaaagccg
     8461 atgaagtgtc gatggaagag gggctggcga aacgcttaaa cgttgccctc ggtgataccg
     8521 tgacttttat gggcgatacc caggagttcc gcgctaaagt gaccagcctg cgcaaagtgg
     8581 actgggaaag tctgcggcct aatttctatt ttattttccc tgaaggggca ttagacgggc
     8641 aaccgcagag ctggcttacc agtttccgct gggagaatgg caacggcatg ttgacgcaac
     8701 tcaaccgcca gttccctact attagcctgt tagacattgg cgcgatttta aaacaggtcg
     8761 gtcaggtgct ggagcaggta agtcgggcgc tggaggtgat ggtggtgctg gtcaccgcct
     8821 gcggtatgtt gctgttgctg gcacaggtgc aggtgggaat gcgtcagcgt catcaggagc
     8881 tggtggtgtg gcgcacactc ggtgcgggga aaaaactgct gcgtaccacg ttgtggtgtg
     8941 agttcgccat gcttgggttt gtttctggcc tggtagccgc aattggtgcg gaaacggcgc
     9001 tggcagtgtt gcaggcgaaa gtgtttgatt ttccgtggga gccagactgg cgattgtgga
     9061 ttgtgctgcc gtgcagcggt gcactgctgc tgtcgctttt cggcggctgg ctgggtgcgc
     9121 gactggttaa gggtaaggcg ctgttcaggc agtttgcggg atgatgagag taaataaggg
     9181 gcgttctggt tgaatggaac gccttgttta aattaattct acaatatgtt tcattgatga
     9241 tgttattgaa ttggtgattt cctatctttc tattgctgat attaatctga atcggtggag
     9301 tttaatgatt ttaaatatag ccctacaaaa ataactcaga ctaataaaat cataaatcat
     9361 atgcgttgaa tggatattat ccatatagtg aatttgttga taatgaattc atctgtgcta
     9421 aaaatgttag tttaataaaa tattgaaagt gacctgtaat aacagttgtt gttgattgag
     9481 aacaaataag tttatgtgaa aaatatataa atacattagc tggtcttgtg tgtcatttta
     9541 tttttttgtt gctaacacag ggatatgaac aataactaaa agggcacttt atatgagcgg
     9601 aaaaccagcg gcgcgtcagg gagatatgac tcagtatggc ggtcccattg tccagggttc
     9661 ggcaggtgta agaattggcg cgcccaccgg cgtggcgtgc tcggtgtgtc cgggcgggat
     9721 gac
//
LOCUS       DAZKGF010000180         9715 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZKGF010000180 DAZKGF010000000
VERSION     DAZKGF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.180"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(88..885)
                     /gene="mtfA"
                     /locus_tag="ACHOQV_004228"
     CDS             complement(88..885)
                     /gene="mtfA"
                     /locus_tag="ACHOQV_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFP9632511.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            979..1068
                     /locus_tag="ACHOQV_004229"
     tRNA            979..1068
                     /locus_tag="ACHOQV_004229"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1013..1015,aa:Ser,seq:cga)
     gene            complement(1738..2388)
                     /gene="zinT"
                     /locus_tag="ACHOQV_004230"
     CDS             complement(1738..2388)
                     /gene="zinT"
                     /locus_tag="ACHOQV_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFP9632512.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2645..3280)
                     /gene="msrQ"
                     /locus_tag="ACHOQV_004231"
     CDS             complement(2645..3280)
                     /gene="msrQ"
                     /locus_tag="ACHOQV_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFP9632513.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSL"
     gene            complement(3281..4285)
                     /gene="msrP"
                     /locus_tag="ACHOQV_004232"
     CDS             complement(3281..4285)
                     /gene="msrP"
                     /locus_tag="ACHOQV_004232"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFP9632514.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTHERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            complement(4394..4807)
                     /gene="hiuH"
                     /locus_tag="ACHOQV_004233"
     CDS             complement(4394..4807)
                     /gene="hiuH"
                     /locus_tag="ACHOQV_004233"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFP9632515.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4940..5611
                     /gene="hprR"
                     /locus_tag="ACHOQV_004234"
     CDS             4940..5611
                     /gene="hprR"
                     /locus_tag="ACHOQV_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFP9632516.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5611..6969
                     /gene="hprS"
                     /locus_tag="ACHOQV_004235"
     CDS             5611..6969
                     /gene="hprS"
                     /locus_tag="ACHOQV_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFP9632517.1"
                     /translation="MKRLSITVRLTLLFILVLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGHGINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDNREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICL
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIQITSFLDTNGSLNIDIASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(7076..7927)
                     /gene="hchA"
                     /locus_tag="ACHOQV_004236"
     CDS             complement(7076..7927)
                     /gene="hchA"
                     /locus_tag="ACHOQV_004236"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFP9632518.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(8519..9592)
                     /locus_tag="ACHOQV_004237"
     CDS             complement(8519..9592)
                     /locus_tag="ACHOQV_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP9632519.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRDVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     GDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARDGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
BASE COUNT         2726 a         2058 c         2189 g         2742 t
ORIGIN      
        1 ggcgcatatt acttagcggt accttgtctg tcaacactaa atttcataag ccatttcaat
       61 tggttaatta atctgcaaag ttgttaatta atgaacattc gtcgccgaaa acgagtctgt
      121 atcattagcg tgatgcagtc tctgcaaagg atcttgttga taaaattgac agaaacgttg
      181 ccacaatgaa gggaaacgag gagcaaaaag ttctggggcg ctaaagaaat attcagaaag
      241 tacggcaaaa cattcagcag gatcactggc agcataagca tcaatgctcg ccgcattctc
      301 accaactaat tcgatttctt cctgaatgtt gttcattgca gcatgaagat cgtgttccca
      361 gccagcaacc tcacgcaacg aaataaaggg aactccgctg gcgcgatcgc cgttacgggt
      421 gtccagctta tgagcgactt catgaataat caggttaaaa ccagaagcat caaaagaatc
      481 ttgtatatcc aaccagttca aaacgatagg cccttgctgc cagctctgac ctgactgaac
      541 aatacgttgg ttatgcacca gaccgatatc gtcttcccat tcatcatcga ccacaaatgg
      601 cgcaggataa attaagactt catgaaaacc atccagccat tccagtccta actccagaac
      661 gggcaggcaa aatagaagtg ctatccggca gcttcttaat gaatccagtt caaagccctg
      721 taaaggaaca agccgctttt gctgtaaaaa acgttcggca agagtgacta atttgctttg
      781 ttcctgttcc gtcagacacg ttaaaagggg gatcgatagt gcttcctgcc agggaagggc
      841 agtttgatgt gctgattctt gtactttcca gggccactta atcatcgttt tgctcgcaaa
      901 ctcgtcactt gaacaaaatt gcacggacag ggactgttaa aatgccaaat ttcctggcat
      961 catggcaacc atctgaacgg agagatgccg gagcggctga acggaccggt ctcgaaaacc
     1021 ggagtagggg caactctacc gggggttcaa atccccctct ctccgccact ttatcaatga
     1081 cttatctccc gacttcccgc cttgcttttc ctaaacagaa caatcgtaga atattcttga
     1141 agggttagat cgtcactgtt ttctgttcga tactgtgaca ttcagcactt gattcgctat
     1201 ggagctgaca ggaaggtttc gagcgaaaat ctacagttat tcagtcgttt tcttatcgat
     1261 caccattatt cttttagaca ttgatcctat gaaactgccg caaagttgct ggcgggaact
     1321 gaagttgcgt agagaagggg tcaataattg agccaaaaaa ggaacgcggc cgtcagatta
     1381 aagccgcggg acaaagtcca taatttccaa gagttgttga atttcccagc ttttttgaaa
     1441 gcctttgtat catactaaca agtaaaaaag gtttaaacac caaaattaac ctatatttct
     1501 ggttagcttt accatgacag atgacttgct cctggaaaca tttgcgctgc cagtctgtat
     1561 ggctgagttg tgtatttatt gttcgccttt ttcgcagttt ttttctacag acataataaa
     1621 aaagacacga ctcaagtatg agcgtgtctg agtttgtctt acttatttgt cttggtaaat
     1681 tattgttgtt actaccaggc actgttctgt taaataactg aagtcagcgc aaaaaaatca
     1741 atgagacatc atctcctcga ccacttcttc gctactcaac tgatagggat aatacgttgg
     1801 ccagttttcc atttcattca ataaagattg ctgggaatca ttacccataa aaatgtgaaa
     1861 atgagatgat tttcgtggtg caattatatg atcgctaaat tgtatatatt taggggcttt
     1921 gctttcagga tctttacatt cgaataagta acgaacgcct ttcttgcctg atttataggt
     1981 gagtattttg tatccatcgt aatcatattt acaggatgtt gtttcattat ttctatggaa
     2041 ttcaacaatg ccgtcctcaa tgccaatcat ctcgatatct gttgcataac ctttgtgata
     2101 ataatcttta atttcagcaa atgttttagt tttatctgca tccgctttct tctgaaagac
     2161 ggggtcaagt ttgccacttt gcaataaagg ataaacggat tgccagactc catcccagtc
     2221 acttagcgtt cggttttgta cattggcatc atcaaaaaca ccattagcag ctttttgttc
     2281 gacctctgtt aagggtttgc cgtgtgagtg atgaccatgc gaaaaggcag gagcgctaac
     2341 aataaagaca cctaaagcaa cagccagttt atgaagacga atcgccaaaa cagtttcctc
     2401 cagagttaat gtatatgatg tgtaatgtta tattgtaaca tataacaaaa tgcaacaact
     2461 cttaggagac aagagaatag ttctctcagg tgtgcttatc tatcttattg aaataaaata
     2521 aattatgtcg atgatttgag gttgatgaca aacgaaacat aacctaatgc gatctgtgta
     2581 tgccggataa ggcgctcgcg ccgcatccgg cagggataac gcgtactttg tcggcaatat
     2641 caagttatag agataatttg ttgtgaacct gcttgcgtaa ccggttgaaa agggaaagca
     2701 acttcttata ccgtaaggct aaaagcagta ccgccagccc agcgtagatg agtggctgcg
     2761 gtgaaataat cttcactgac cacagataat gtattggggc caggatcgcg acaagataga
     2821 cgaaattatg caactgttgc cagtgcttgc cgagttttcg ctgcatcgcc tgggttgaag
     2881 taaacgctaa agcaagcaaa ataacccagc tgataattcc taacgttaaa taaggccggg
     2941 taattaactc cttacctaat aacgccagat tattcacgcc cagctccagt aatgcgtaac
     3001 tggttaaatg cagtgtcgcc caggcaaagc accataagcc taacaggcgg cgagtgcgta
     3061 tcaataacgg ctgttttgcg tagcgtgcca gaggtgtgat taacaaggcc gcaagcaaaa
     3121 atttcagtgc agtgcgaccg gtaaaatgct gaatatcttt caccggatcg gcacccaatc
     3181 caccgtgatt aatcgcccag accagccaga gaaacggcaa caatccggca agatgcaggc
     3241 aaactttcag ccatgtcacc tgttttgctg tcagacgcat ttagaaattc tcccgcaaat
     3301 ccaggccacg atacagcgat gccacctgct cggcgtaacc attaaacagt agcgttggct
     3361 ggcgctgtac atcgaggatg ccgcctgaac caataaatcg ttcggtagcc tgtgaccagc
     3421 gcgggtgatc aacatgcgga ttaacgttgg cgtaaaaacc gtattcgtca ggcgctgcca
     3481 gattccaggt ggttggcgga cgctcgtggg tcagcttaat actgacgatc gatttaatcc
     3541 ctttaaagcc atatttccac ggcacaatca gtcgcaccgg cgcgccattt tgtggcggta
     3601 acgccttgcc ataaacacct acggtcatca gtgtgagcgg atgcattgct tcgtcgagac
     3661 gcaatccttc gacataagga tatttcagcc cgccgccgat aaagcggtcc tgctggcctg
     3721 gcatctgttc cggtgcataa attgtttcga aagcgacata cttcgcattg ctggtgggtt
     3781 cggcaagcgc cagcaatttg tgcagcggaa aaccaatcca cggcaccacc atcgaccatg
     3841 cttccacgca gcgcatacga taaatacgct cttccagcgg gaagcgacgg gttaaatcat
     3901 cgtgatcgag ggttaatggt tttgctactt cgccgctgat tttcagtgtc catggatcgg
     3961 ttttcaggct accagcatta gcggcgggat cggctttatc cagcccgaat tcatagaagt
     4021 tgttataacc ggaaacttta tctgctggcg tcagtggcag gttattttgc caggcggcag
     4081 gcttgctgaa ctccagcggt tttccggcgg gggcgggtgg acgatcgttc cctttaaacc
     4141 agctaagcag atcggcatgc gcagcgtgag gcaaagatag tgcagccgcg ctgatgccca
     4201 gtgcttttaa cacctgccga cgcttcataa agaataccga ctcggccgta acatctgatt
     4261 cttttaaaaa ttgattcttt ttcataagcc atcacacttt tttgtcgtta gccagcaaga
     4321 gccgtttact gttaatctgt cagggagtca cctccctaac agtagcagag atctgctttt
     4381 atatatttat gcattaactg ccacgatagg ttgaataccc atattggcta agtaataaag
     4441 gcacatgata atgctcgttc actttattaa tatgaaattc aacggggatc tcagggaaga
     4501 aactttcaag attttgtttc ttgaaatagt ccccggtttt aaatacgaca cggtaatcgc
     4561 ccgtagtcgc agtttgctcg ggccacagtg ccttaattcg tccatcctta tctgttttgg
     4621 cggtattaag ttgtaaccag ccgttatccg ctttcttttc aagagtgact gtcacgtcgg
     4681 cagcgggttt tcctgtttgc tggttcaaaa tgtgcacgct aagaatgttt tgttgtgcag
     4741 catagaccaa agaaggtaat gaaaatgcag ccgttactac ggagagtact aaataacgct
     4801 ttaacatgtt tatatccttg tcatgtgaat gagttttgag gatagcgaaa aagcgtccag
     4861 ggtaacctta cagcagcatt acaattttgt aatgaaccag gctgtttcta taacatatga
     4921 tttatggcat attattttca tgaagatttt acttattgaa gataatcaaa ggacccagga
     4981 gtgggtaacg caggggcttt ccgaagcggg ttatgtcatt gatgccgttt ctgatggcag
     5041 agatgggctt tatcttgcgc tgaaggatga ttatgcattg atcattctgg atattatgct
     5101 tccgggtatg gatggctggc agatcttaca aacgttaaga acggcaaagc aaactcctgt
     5161 tatttgcctt acagcaaggg attctgtcga tgacagagtc agagggctgg acagtggagc
     5221 aaatgattat ctggtaaaac ctttttcatt ttctgagttg ctggcaaggg ttcgggcaca
     5281 attaaggcaa catcacgctt tgaattcaac attagaaatc agtggcttaa gaatggactc
     5341 tgttagtcaa agtgtgagca ggaacaatat cagtattaca ctgacgcgca aggagtttca
     5401 gttactttgg ctactggcct ccagagctgg cgaaattata cccagaacgg ttattgcgag
     5461 tgaaatttgg ggaatcaact ttgatagtga taccaatacg gtggatgtcg ccattcgcag
     5521 gctccgcgca aaagttgatg atccttttcc tgaaaagctc attgccacaa tccgtgggat
     5581 gggatattca ttcgtagcgg taaaaaaata atgaaaagat tatctataac cgtccgttta
     5641 accttgcttt ttatattggt gctgtctgtt gctggcgctg gaattgtatg gactctctat
     5701 aatggcctgg caagtgagtt gaaatggcgc gatgatacaa cactcattaa ccggacagcg
     5761 cagatcaagc agttgttaat tgatggggta aatccagata cgttacctgt ctattttaac
     5821 cggatgatgg atgttagtca ggatatcttg atcattcatg gtcatggcat caataaaatt
     5881 gttaaccgga caaatgtcag tgatggcatg ttaaataaca tacctgctag tgagacaatc
     5941 agcgcagctg gcatttacag aagcattatt aatgacacag agatagatgc tttacgaatt
     6001 aatattgatg aagtttcgcc atcattaacg gttactgtgg ctaaattggc ttcagccaga
     6061 cataacatgc ttgaacagta taaaattaat agcattataa tttgcattgt cgccattgta
     6121 ctttgctcag tattaagtcc gctgttaatc agaacggggt tacgagagat caaaaagttg
     6181 agtggtgtaa cggaagcgct gaattataac gataaccggg agcctgttga ggttagcgca
     6241 ttaccgagag aactaaaacc tcttgggcag gcgttgaata aaatgcatca tgctttagtc
     6301 aaagattttg agcgtctaag tcagtttgct gacgatctcg ctcatgaact tagaacacca
     6361 attaatgcat tactgggtca gaatcaggtt acgctcagtc aaaccagaag tatcgctgaa
     6421 tatcaaaaaa caattgccgg aaacattgaa gagctggaga atatttcgcg gttaacagag
     6481 aacatactgt ttcttgccag ggcagataaa aacaatgttt tggtgaaact ggattcgctt
     6541 tctctcaata aggaagtcga aaatttgttg gactatcttg aatacctttc agacgagaaa
     6601 gagatttgcc ttaaggtcga gtgcaatcag caaatctttg cggataaaat tttactgcaa
     6661 cgaatgttat cgaatcttat tgttaacgct attcgatatt caccagaaaa atcgcgcatt
     6721 cagataacca gttttcttga taccaacggc tctcttaata ttgatatcgc cagtcctgga
     6781 acgaaaattc atgagcctga aaaactcttc cgtagatttt ggcggggaga taattcgcgt
     6841 cattccgtag gtcagggact tggtctttct ttagtcaaag cgattgccga attgcatggg
     6901 ggaagtgcta cgtatcacta tctcaataag cataacgtgt tccgaattac gttaccgcaa
     6961 agaaattaat atagggcttc agtacgccaa aattaaccgt gaaaaaacgc agtcacgtcg
     7021 tgagtattaa cgtggctgcg attgataatg cgcttacatt acaatgtaac agggattaac
     7081 ccgcgtaagc tgccagcatt tcctgcgccg ccagtttacc caacgcattc gctgcgaaag
     7141 gactgtcgcc ggtgagaact ttacggtcct tatgtactcg cccggtgatg tcgtcattaa
     7201 tgatgttcat gcccattttc ttcagctctt cgccgaagta ccaggtgaga tgacccggca
     7261 tatagcctat ctctggcgtt tgtttgtctg cggcgtctgg gaatgcgcaa atggaataac
     7321 cattcagtgg gttatcgccg tggcgaagcg ccagaaaagc cgccgggccg tggcaaaggg
     7381 agataacaaa acggtcattt ttgattgccc actgtaaagc ggcagctacg tcctggcttt
     7441 caggtaaacc aataagtgcg ccatgaccac caggaacaaa gattgctgca tattcgctat
     7501 cagcgttgag gcttgcgaca acatccgcga gtttcttcgg attgcggaac aacgatttat
     7561 gctgctcaaa gaatggcatt actttttcat ctttgtgtgg catagcccag tattcaaact
     7621 tggtcatcag accggaaatg gtcgccactt cgaattcgaa acctgcagca tggagatgat
     7681 acaacggcag caacgtttca atcggatggt taccggtcga gaatagtttc ccgttatcgg
     7741 tcggcaaata acgttcgtcc gcggcgatca ccagaatttt atgtttaccg cgatacggtt
     7801 ttggatagtc cacgccatca agatcagaga cgggactggt atattggcta agcgaatatt
     7861 ctgaagggaa gaatgcatta tcttcagcaa tatcgacctg cgggttttta cttgtttgaa
     7921 cagtcatagt gtattcctta ttgttgctta gttagggtag tcactatatt tgcgactgag
     7981 ctggtcgcta tttttaacca ggctgaaaaa ttgagtcaat atatcgaaag cggtagttgg
     8041 cgggcataag tatgtaatga tgatgctgca ctggtgttga atattattca cgccaaattt
     8101 tccttattga gctacgtcgt cgcaagtggc ctcaatgatt ttagaccatt atcagaggtc
     8161 cggggcggca gggagagatt tagtgctgtg gaatattgtg gtgctgatga atatttatat
     8221 cgcggattgg tagagaaaaa gcttttttca tgcactattt ttgcaaatat ttctatatgg
     8281 acggcggttt tgttgcggat taattcataa agaaatgcat ttggatgttg gccggaagag
     8341 acgctggtag attttattga gccaggaggg gtggggtaag gtgctgtatc gatatgcttt
     8401 ggcaattgcg tgtgccgcag atggagatga aatgccaata aaaaagcctg agttcgatga
     8461 gggacgaact caggctggtg ttaagtttta cagtaacacg ccgcggcgcg ttggctgatt
     8521 agaactggta gacgatacca actgcaaact ggtcatcagt tgcggcatcg gtaatcgctg
     8581 catcgtcttt atccagcagg ttaaatttgt atgctgtgta gacgttcatg ttcttattga
     8641 agtagtacca ggtaccaact tcgatataat ttacgcggtc agcatcaaca cctttatgtc
     8701 catcacgagc ataaagatct ttgcctttgg aatatacgta gcctaaagac ggacgtaaac
     8761 cgaagtcgaa ctgatactgt acaacagctt caaagttttg ggttttattt gcaaaatgat
     8821 cattagccaa aacggtcatg ttgcgggttt caccgtacat cattgctgca tagatattat
     8881 ttgcatcata cttggctgct aatgaccaca cttcagcgtt atcaccgtag ccgtcaccag
     8941 cttgttcatt agtacgatca gatttgctat atgcacctac aaaaccaaaa ccttcgatgt
     9001 tgtagtctac agacaacgca tagccatcac cgttttgttt cgtaacgtca cgattgctgt
     9061 tcttaccttg atattgcaca gcaaaattca gaccatcaac cagaccaaag aaatcagagt
     9121 tacggtaggt tgcaacaccg ttggtacgac cgttcatgaa gttgtcagcg gacgcccagg
     9181 aatcaccacc ccattcaact aacatatcgg tataagctgc ggcatcataa gcaacaccgt
     9241 agttacgacc atagtcgaaa gatcctaact cgttatattt caaacctgcg aaggttaaac
     9301 gggttttttc ctgattagag ccttcgggct tgctggcgtc gaggttgtat tcaaactgac
     9361 caaaaccagt cagttcgctg gtgatttggg tttcaccttt aacaccgaaa cgagcatagg
     9421 aggtatcttc gttttcgctg ttattatcat cagtatttga ccagatgcgt tcgccaacca
     9481 tcttgccgta gaaatcaact ttattgccat ctttattata aatttcagcc gcatttgctg
     9541 cgccagcaac taataacgcc gggaccagca ttgccagaac ttttcttttc attatgtatt
     9601 cccttgtgat tataatcttc atgaatatat caataagtgc cgttatccaa aaaaaagcac
     9661 atttggatac tattctatga agttcatttt attttaaaga ttacatgtaa caaat
//
LOCUS       DAZKGF010000181         9667 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZKGF010000181 DAZKGF010000000
VERSION     DAZKGF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.181"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(317..1921)
                     /locus_tag="ACHOQV_004238"
     CDS             complement(317..1921)
                     /locus_tag="ACHOQV_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP9632520.1"
                     /translation="MNPDYVQALQTSPHFPTDVTYRLFPSDLAYLIDDLYSSTQLPLE
                     LIFNTVLATLSLSCQSLVDVVHPHTNMPEPCSLYLLAIAEPGAGKTTINRLVMTPCYE
                     FADRLIQQYEERNKDYKTELQIWNTRQKALAANLRKAVNRGDPGEQEEEALRNHERNK
                     PTRPVRPNFIYEDVSLKALAEGLNEHPEAGIISDEAVTFFRSYLKNNPGLLNKAWSGQ
                     PFDFGRADEKYHITPRLTFSLMSQPNVFTDYINKNDVLAWGSGFLSRFLFSQVGGSSR
                     VRDYRKGEFVTIPTLVEFHKKINGFLSSHSINSPGMRSERKTLKLSKKALGEWQENQI
                     KIESKALAGGEWEHIRDIVLKAGSNILRIAGLFTCYCYKDDEEITSIALWKAMHLMEW
                     YLEEASTIFYPMSERCQFEQDARELYTWIMTRIKQNNLYAIRKTDIERYGPNRLRRAE
                     KLTPVLNQLIAQNYLCIIKMRSHQALYVSVHCNNRYLLPCGAMSYEQFDIVPPLNSHN
                     AKTYHVNIPLALIPSFALPFSAYTLF"
     gene            complement(2351..3256)
                     /locus_tag="ACHOQV_004239"
     CDS             complement(2351..3256)
                     /locus_tag="ACHOQV_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9632521.1"
                     /translation="MALEQEIANLVNDFVAGGRPSCRDQTFDERRAGYIASTALAGDT
                     ETRVNISDVIIDDILLRIFSPLNDDKLSSAIVYYHGGCFVSGGFVTHDNQFRQLAFLS
                     GCRVIAVQYRLAPEHIYPAAHDDAEHAANIVWQHAEMLKVDRNKITLMGDSAGGHLAL
                     ITALRVKNIAQWLPRKLILIYPMLDATASCDSYKLNGSDYVLTEDTFLSGYEAYFSGL
                     DLEHPEASPLFRKDFNGLPPVHIITAEYDPLRDEGEELFSRMIQQGVKCTAQRYLGVI
                     HGFFQLGGISQTARNLMQDICGIVE"
     gene            complement(3710..4162)
                     /locus_tag="ACHOQV_004240"
     CDS             complement(3710..4162)
                     /locus_tag="ACHOQV_004240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP9632522.1"
                     /translation="MNVIDSGIRDVKFGENVKVVMPVNLYECELGDDVFIGPFVEIQK
                     GCIIGNRSRIQSHSFICENVILGDGCFIGHGVTFANDLFKCGGPDASAESWIRIVLGN
                     TVTVGSGATILTTKICSGAVIGAGSVVTKPVLVKGVYAGNPARLLRTL"
     gene            complement(4676..5407)
                     /locus_tag="ACHOQV_004241"
     CDS             complement(4676..5407)
                     /locus_tag="ACHOQV_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFP9632523.1"
                     /translation="MNQSIHNAYWLSRFDSLLDSAPAQHRAVSLIRVDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRKKRCANQRVHATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDIWCSLGDFSEPSSLATMIQQAWCSALHPEPWQGDGLVHFSRWTPSRKPA
                     AADASPSSDDTPLHSGCSDTRTDSDKRPGGSAVLWAKRGDVEAMQKALERARYLVKNE
                     TKLHDGSGQRNYGCSRGVRCLLDGR"
     gene            6078..6608
                     /locus_tag="ACHOQV_004242"
     CDS             6078..6608
                     /locus_tag="ACHOQV_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9632524.1"
                     /translation="MKLTEESLNLVIDILNDLICEGKMLNDVERRSLLNAAMTITAVK
                     ERSVVSVSSRKEQSKKEKKEREIDPRFPNAGVAWNEADESLLRDVLEDVPDDEIGKHL
                     FWLAEKLGRTPYSVACKVRQIKKLPAEWKDQFRKISDRIRSSGMSVSEYLQRQGLLSP
                     ESGEENNPVSHNNNVA"
     gene            8288..9091
                     /locus_tag="ACHOQV_004243"
     CDS             8288..9091
                     /locus_tag="ACHOQV_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632525.1"
                     /translation="MSADGISDLIKPEIFLVIFPGTTTTAKRGGAGWLNNKRVVFRVW
                     LYISKASYKKMGIFQRGSAPESIPYREYHFPSYPDTHEKRKKVLRDWISFMRSRFNIN
                     QIAQFCYRMENEWAFTFDNIHVPRHFREGTDDTEWSWNYIKKKRLFSLSGLQHIQPQN
                     NTERKLLINAFFDTCLIENNPADDIDIKNMLLRKMNAAFYKRKSRARARIHSRNNQQM
                     SVSSGTDKQVNIRLKDFQYQQLLFIAQKENKSKSEIIKELITRAYRAIQ"
     gene            complement(9148..>9667)
                     /locus_tag="ACHOQV_004244"
     CDS             complement(9148..>9667)
                     /locus_tag="ACHOQV_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632526.1"
                     /translation="KVSSLMGDSELRYVYSFPGSLYSYLLEKHPLSEVKKLMEDLPST
                     EDENFRRSETGSQYLKGILGVKTRVSELFIRQFSLPFRFPHTKEYVRDVIFKPVENDM
                     SLSEELAHVLFFTLLHSDIMSRFSLRELFFRIESGRDNNKKRDLIREYVSDVTKEYSL
                     CIPGIVSRHDKK"
BASE COUNT         2934 a         2195 c         1871 g         2667 t
ORIGIN      
        1 taattagctt caatccatgt gaaaaataac atttcgattc actttcgaga tatcaaaaac
       61 gaaaacaatt cttccaccag tgcacacatt cctgcattca accagctctg acaacaggtc
      121 attatgaaaa caaaatcaaa tcacatacat ggtacatgta ctgctaccgt tgctaccgta
      181 gcgatttttt cagagaaatt ttcgttcaca ccccataatt ggtgaatatt tgcacaaatg
      241 caggatatga aaaacgcctt accatcaacc ttcgaatgaa ggcttatgtg ttttgtttgc
      301 ttattgtaaa gctgtttcaa aataaggtat aggctgaaaa aggtaacgca aatgacggaa
      361 ttaatgccag agggatatta acgtgatatg ttttcgcatt atgactattc aagggtggaa
      421 caatatcaaa ctgttcgtaa gacattgcac cgcaaggaag gaggtaacga ttgttacaat
      481 gaacagaaac ataaagtgcc tgatgcgatc gcattttgat gatacataaa taattctgtg
      541 cgattaactg attgagtaca ggtgtaagtt tttctgctct tctcagacga ttggggccat
      601 atctctcaat gtctgttttc ctgatagcat acaaattatt ctgcttgatt cgggtcataa
      661 tccatgtata cagttcacgg gcatcctgtt caaactggca tcgttcagac ataggataaa
      721 atattgtgct tgcctcctcc agataccact ccatgagatg catcgctttc caaagcgcta
      781 ttgatgtaat ttcctcatcg tctttatagc aatagcaggt gaataaaccc gctatcctca
      841 gtatattaga acctgctttc agaacaatat ctctgatgtg ttcccattcc ccacctgcaa
      901 gcgctttgct ttcaatttta atctggtttt cctgccactc tcccaacgct ttcttcgaaa
      961 gttttaatgt cttcctttcg cttctcattc cgggggaatt aatgctatgg cttgacagaa
     1021 acccgttaat ctttttatga aactccacca gggttggtat tgttacgaac tcgcctttcc
     1081 tataatcacg tacccgggaa gagcctccaa cctgactgaa aagaaaacgg gaaagaaatc
     1141 cgcttcccca tgccagtaca tcatttttat ttatataatc cgtaaaaaca ttcggctgag
     1201 acattaatga aaatgtcagg cgtggagtaa tatgatattt ttcgtctgct ctcccaaaat
     1261 caaacggttg tccactccat gctttattca acagacccgg attatttttc agatagcttc
     1321 tgaaaaaagt taccgcttca tcagaaataa tccctgcctc cggatgttcg ttgagtcctt
     1381 ccgcaagcgc tttaagcgaa acatcttcat agataaaatt cggtcgaacc ggacgtgtcg
     1441 gtttatttct ttcgtgatta cgcagtgcct cttcttcctg ttctcccgga tccccccggt
     1501 taacagcctt tcttaaattg gcagcaagag ctttctgccg ggtattccag atctgtagtt
     1561 cagtcttata atctttgttt ctctcttcgt attgttgaat gagtcgatcg gcaaattcgt
     1621 aacagggggt catcaccagt ctgtttatcg ttgtttttcc cgcgcctggc tctgcgattg
     1681 ccaacagata aagtgagcag ggttccggca tgttagtgtg aggatgaaca acgtcaacca
     1741 atgactgaca ggagagtgag agcgttgcca gtacagtatt aaaaatgagc tccagaggaa
     1801 gttgggtact tgaatacaag tcgtcaatga gatacgcaag atcactagga aataatctgt
     1861 aggttacatc tgttgggaag tggggggatg tctgcaaggc ttgcacataa tcgggattca
     1921 ttgtaatgga cctccttcac atactgtaat cagcacaaag cttattgttc agttcagaaa
     1981 cataccaaca ttgtgagtat gttcgctgac gccaataata aaatgactga tctccattca
     2041 ccagtcccgg cgcatttatg gttctgacaa aagtaaacaa catgggagcc agaactctgc
     2101 atcgcagcga caaactatgg atgcatgaaa aacagggaac attacgcacc agagcgttga
     2161 gagtcttcct ccttgcccaa tacgaaagtc atctatccac ccgtcttcaa tcttcgggat
     2221 ggttgtttta tgaacagagt aaaagtttga agcaagctga gaacctctgt ttccacgtaa
     2281 aggtccgcta tgaagaaaaa tgggagtttg catgctcaac taatttacct gtaggtgctt
     2341 gttactgtag ttattcaaca atcccacaaa tatcctgcat cagattacgg gctgtctggc
     2401 taatgccgcc aagctggaaa aaaccgtgaa tgacaccgag gtaacgctgg gcggtacact
     2461 ttactccttg ttgaatcata cgggaaaaca gttcctcacc ttcatcgcgt aatgggtcat
     2521 attccgctgt gatgatatga acaggtggta acccattgaa atcttttctg aataatggac
     2581 tggcttccgg atgttctaaa tccagaccgg aaaaataagc ctcataacca ctaagaaacg
     2641 tgtcttctgt cagcacataa tcacttccgt ttaatttgta actatcgcat gatgctgttg
     2701 catccagcat agggtaaata agtattaatt ttcgtggtaa ccactgagcg atgtttttca
     2761 cccgcagtgc tgttatcagt gcaagatgtc cccctgcgct atctcccatt aacgttattt
     2821 tgttgcgatc aactttcaac atttctgcgt gttgccatac aatattagcg gcatgttcag
     2881 catcgtcatg tgccgcagga taaatatgtt ccggcgctaa tcgatattgt acagctatga
     2941 cacgacagcc gctcaggaaa gcaagctgtc gaaactgatt atcatgggtt acaaaaccac
     3001 cactgacaaa acagccacca tgataataga caattgcgga tgacaattta tcatcgttta
     3061 aaggcgaaaa gatacgcaat aaaatatcat caataatcac atcactgatg tttacgcggg
     3121 tttctgtatc gccggccaga gcagtacttg caatatatcc cgcccgacgt tcatcaaatg
     3181 tctgatcacg acaggatgga cgaccacccg caacaaaatc atttacaagg tttgcgattt
     3241 cctgttcaag tgccatacaa atcaactccg accgataata tttaaacaac gctacgggaa
     3301 gtataaatac tctgaccaaa atataacagg tgaatatcca tagcttcaac aggcgtaatc
     3361 aataagggcg tacggagctt gcccgttatt gctgcaggat cgacagactg gttttcgtcc
     3421 ttgcgcatgt aacagtacaa ccacagagaa aagcatcaat ggacacttta tgttgcgtca
     3481 gggcagcgaa cgactccttt tgttgacgac acatatcacc ttctgtactg gcaattacgg
     3541 cattcagagt ctccctgagt tttaagctcc tgctcttacg gaccagcgac aagcaataaa
     3601 taactatgac aatgaatcta ataacagcat aaatatgata ctcatcgtga ttagtataat
     3661 cggggcagaa ttatattcac tgtctgattt attattgggt taaatataat taaagtgttc
     3721 tcaaaagtct ggctggatta ccggcataca cgccttttac cagcacgggc tttgtaacca
     3781 cacttccagc accaatcact gctccactgc aaatttttgt tgttaaaata gtcgctccgc
     3841 tacccactgt tacagtgtta cctaatacga tacgtatcca gctttctgca gaggcatcgg
     3901 gaccaccaca cttaaaaaga tcattagcaa aagttacgcc atggccgatg aaacagccat
     3961 cacctaaaat gacgttctca cagatgaaac tgtgggactg aattcggctc cggttgccaa
     4021 tgatgcatcc cttctgtatt tccacaaagg gaccaataaa aacatcatcc cccagttcac
     4081 attcatagag gtttactggc ataacgactt tcacattttc accaaatttc acatcacgaa
     4141 tgccgctgtc aataacgttc atatccgttg tatcctccct caggatgatt cttatcagaa
     4201 catttcacct gtcagaatat tcccggtaat gtgatacaag atccagtctc accgggtacc
     4261 acgtgttatt gcccgtatcc ttttgcccgt tacgagtcta caactggttt tgtattttgc
     4321 aatccaggcc tgttacacca gagcatcctc tcacttcccc ctcactggtc agtccgggat
     4381 tcaacatcat cctccagcac taaccctata ccagtcctga accttccctc gccagcagcc
     4441 tactgttcac cacaatcctc ttccgtgaat ggcagtaagt gagctgcacc aaaataaatc
     4501 ggtacttccg atgacagaca gtggaagtgc caggagaaat atgaacatga gaatggaagc
     4561 agataaaaat accgttgcat gacttcagga cgggttatca gaatacgagc taagggcgtc
     4621 agccaacgcc cttaccgtgg tatacactca ggcaccggat accattttac cgggttcacc
     4681 tgccatccag tagacacctc accccacgac tgcaaccata attacgttga cctgaaccgt
     4741 catgcagctt cgtttcattt ttcacgagat aacgggctct ctccagggct ttctgcattg
     4801 cttccacatc accacgcttg gcccagagca cagcggatcc acccggcctt ttatcagaat
     4861 ccgtccgggt atcagaacat ccactatgca aaggcgtgtc atcggaagaa ggggaagcat
     4921 cagccgctgc tggtttacgg gaaggcgtcc acctggaaaa atggaccagg ccatcgcctt
     4981 gccagggctc aggatgcaga gcgctgcacc atgcctgttg gatcatcgtc gccagcgaag
     5041 acggttcgct gaaatccccg agcgagcacc agatatcttt gttgagcagt aatatcacgt
     5101 gataatgttt cctgcctttt tccttgccaa actcccgaca ccagaaataa cgcagggtgg
     5161 ttgcatgcac acgctgattg gcacatcttt ttttccgctg gtaagcctga attttggctt
     5221 tcagggatgc aaaaaaacga gaaatcaccg ctgaatccgg atcgggatcc atgatggtga
     5281 caggcatata ctcagggaaa cgtaaatcca cccggattaa cgagacggca cggtgctgcg
     5341 ccggggcact gtcgagaagg ctgtcaaaac gggataacca gtaagcattg tgtattgatt
     5401 gattcatgat gagagatctc cgttgatttg gattgacttc acatcattca tcaacgcggt
     5461 gaaactttac ggcatcccgc caatgagcaa cgccaatagc tcctgattaa aacggaatga
     5521 atcgtcccgt aacgaaaaga taatcctgca cacgacagca aaacgtaaaa caacagcatg
     5581 aatgagctaa atgtttaaag aaaaaatgtc ttttcacatt atttttcagc aagtaatcat
     5641 accagcatcc ctaacatacg aaatcacata gtgatcgatc gctgattcgc ttaacaactg
     5701 atcgaataca ccggaattta cactaacatc ctgatgggta acggaagact aaaaaacctg
     5761 tgttctattg attgcacgga atgcctgtgc actggcctga ccagtaagca caattctacg
     5821 agtagtaaga tatctacgcc agaaccatgc ggcagtagcc accatatgtt gccagggtga
     5881 aaaataagct cagagctata ggggagtaat tcccgttaat gtcatcgctg tacctcaatc
     5941 agttcacaaa ctggctgaaa cgttaagctg aagcctttat tcttcagatg tattttccgg
     6001 tttacctgta aattatcttc tgatatttat cacattagta gttgtgctga ttatctacat
     6061 acttcaaggt ctgaaatatg aagctaacag aagaaagtct gaatctagtc attgacatcc
     6121 tgaatgattt gatctgtgaa ggaaaaatgc tgaacgatgt ggagcgcaga tctctgctca
     6181 atgcagctat gacaattaca gcggtaaaag aacgttctgt cgtatctgta tcatcccgaa
     6241 aagagcaaag taagaaggag aagaaggaaa gagagatcga tcctcgtttc ccaaatgctg
     6301 gtgtggcatg gaatgaggca gacgagagtt tattgcgaga tgttctggag gatgtaccgg
     6361 acgatgaaat tggtaaacac cttttctggc tggcagagaa actagggcga accccgtaca
     6421 gtgtagcctg taaagtcaga caaattaaga aattacctgc agaatggaag gatcaatttc
     6481 gtaaaatctc tgacaggatc cgtagttctg gaatgtcagt cagtgaatat ctacaacgcc
     6541 agggactgtt aagcccagag tcaggggaag aaaacaatcc tgtcagtcat aacaacaatg
     6601 tagcttaatc ctgaagaaga tgaacagttt atactcttta aactgttccc ttaattttag
     6661 aaaaacagtt tgtgtaagtg catgctccaa cattctgaag gagttatttc ttacaataca
     6721 tcataatcat tggcagatcc tcatctgaat caaaacacca caaccaatca tcacactttt
     6781 tctgaaaaga gccttatgat gaagataatc accttacgct gagaaaaaac accagggcca
     6841 gttaatcacc ttatcaatct cgccgttaca aaatcatcca taagccattt aacagtgtta
     6901 tcgcaaactc acagtaaatc gcatcaggtt acctcaatcg gatttgagtt aattcactgg
     6961 gctttacgta aagatacttt tctcaggtta acgactaaaa cacgaaaact cagaaaaggt
     7021 taaacacagg ttcagtaaaa tcgaatatca tcttgattgc ttaaacagga cagagggttt
     7081 tatagtgagg ttaactgatt ttagcgatcg taaaaaaagg actaacaaat ttactaaact
     7141 ggatgtgtat cagacaaagc aatgtgtata gcaatgctta atgaaaatac ttgtatgatt
     7201 gtctatgaaa gtacgtgtat atagtatcat accgacattc tcaactcttc caaacatcca
     7261 tgcagactga atgttggttt cagtttttca cggccagcat ggggattttt gttctgaaaa
     7321 cggagcagat ttactctgga attattttcc ctgtcgggat atttttcacc tgaaaacttt
     7381 ctacaacaca acggctgtat aacatcattc tggcatattc attcaggtga gtcggacaag
     7441 cgataatcct gttctttacc gaacgaacag gcagcgataa aaaactgatt cagacgacat
     7501 ttctgtctga caaacattca catgcagaag accgggacat ttattcttcc cgaaaggaag
     7561 tgataccttt tcttatcatg gtagctgcat tacctgatat ctttcaggcg aatcccctcc
     7621 ctgtaaatct gatcgtcaga taaaagagat gaacatcagg cagatttccg tcaataacga
     7681 taagagatga ccatcgtaaa catatcttcc agtaacgaac tggcacctgc atattaccgt
     7741 ttattctcat acatcattga aatattgtca gttataaagg acacacatca ggaaattatc
     7801 accatgtgac aacgtcacag atggtgctga tatataaaaa ttaacaaaat cgcgccaaaa
     7861 aaccgcgccg atattgcgca tctgtaatcc atcatttcct ttttgaataa ccttccgtgg
     7921 ttacccgaca tcctgataat gacgcccatc gtacacgctt ttctttgctc agacatcgca
     7981 tacttttatc ctgctcaata ccgtcttcct gccagtcttc ccactctctg acactcacag
     8041 aacagcaacg tcctccggac atactagcag ggcagaacac aatccacaac attaagtata
     8101 tgtaacaata atataccatt aagaccactt ctatgacctt atatgttaca accttagagc
     8161 atgtatttaa tatcccataa tacagactcg tcattgttct tctgagacga catgggcatg
     8221 tcacaaaact aattgactga catatcatga aaatgtcagt atttaataat ttaatggagg
     8281 tacaaacatg agcgcagatg gcatttccga tttaataaaa ccagaaatat ttcttgtcat
     8341 ttttcctggt acaacaacca cagcaaaacg gggtggcgcc gggtggttga ataataaaag
     8401 agtcgtgttc cgggtatggc tttatatcag taaagccagt tataagaaga tggggatttt
     8461 tcagcgcggt tctgcgcccg aatcaatacc ctatcgtgaa taccattttc cctcataccc
     8521 tgacacccat gaaaaaagga aaaaggtatt acgtgactgg atatccttca tgcgctcccg
     8581 ttttaatata aatcagatag ctcagttctg ttatcgtatg gaaaatgaat gggcattcac
     8641 ctttgataat atccacgttc cacgacactt tcgggaggga actgatgaca cagaatggtc
     8701 atggaattac attaagaaaa aacgattatt ttccctgagc ggcctgcaac acattcagcc
     8761 tcagaacaat actgaacgaa agcttctcat taatgctttt tttgatacct gccttattga
     8821 aaacaaccct gctgacgata ttgacataaa gaacatgtta ctcagaaaga tgaatgcagc
     8881 gttttataaa agaaagagcc gggcgcgtgc gcgaattcac tcccggaata accaacagat
     8941 gtcagtcagt tcaggaacag ataaacaggt aaatattcgt cttaaggatt ttcagtatca
     9001 gcaacttctt tttatcgcgc agaaagaaaa caaaagtaag tcagaaatca taaaggaact
     9061 cattaccagg gcataccggg cgatacaata accaccacgt tcaccatttc atcccgacca
     9121 tacatcatat gcacgggatg atactgacta ttttttatcg tgccgactga ctatgccagg
     9181 tatacataac gaatattctt ttgttacatc gcttacatat tcgcgtataa gatccctttt
     9241 cttattatta tcacgtccac tttctattct gaagaataat tctcttaaac taaacctgga
     9301 cattatatcc gaatgcaaca atgtaaaaaa taacacatgg gccagttctt cacttaacga
     9361 catatcattt tctacaggct taaatataac gtctcttaca tattctttag tgtggggaaa
     9421 tctaaacggc aaagaaaact gccttataaa aagttcagat actcttgttt ttacaccgag
     9481 aatccccttc agatactggc tacctgtttc actcctcctg aagttttcat cttccgttga
     9541 aggcaaatcc tccatcagtt ttttaacttc tgacaatgga tgtttctcaa gaagataaga
     9601 atataaacta ccgggaaatg aatacacata acgaagttct gaatccccca tcagagatga
     9661 gactttt
//
LOCUS       DAZKGF010000182         9647 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZKGF010000182 DAZKGF010000000
VERSION     DAZKGF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.182"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..607)
                     /locus_tag="ACHOQV_004245"
     CDS             complement(<1..607)
                     /locus_tag="ACHOQV_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFP9632527.1"
                     /translation="MTSEVKLKTGGDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTASWYTIARMHTAGLSGLNEGLALIVALTRHHWSVMWPLNTHAR
                     LEIITGLFNRLQKTLRAMPPDDRDNLPLLYQTETFLKALSDTLARHELKQGSKVALPE
                     AMVKGYITRLENQPVQGEASSPVTLPAQALRSDAPDVQEHQT"
     gene            complement(662..967)
                     /locus_tag="ACHOQV_004246"
     CDS             complement(662..967)
                     /locus_tag="ACHOQV_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632528.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRLFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVLH"
     gene            complement(1033..2778)
                     /locus_tag="ACHOQV_004247"
     CDS             complement(1033..2778)
                     /locus_tag="ACHOQV_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9632529.1"
                     /translation="MTPGERRVASRLESFLNDDCLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVSTLRQVNQEQVTLETDGLLKSEINPLVQVRRYACDTVNALPANPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNDENAVEKIFPSAQTICQDEMTQS
                     VLPEVFQQKIAGMFTTGFRTRVTPRMRDILRAHLFPEVTVKQNSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIEARGL
                     ASRVTVSHFHSWCASMVKRHGIKVTADRKEYPDKCFSALEEAVNSGKITRTGYDAVLV
                     DEGHDFDSRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKRILHFAYAFSREYFEKHQNREIPLVQPQAGGEEGTEPEILRCASERDE
                     ARQVADWLEKRHALCGHWSDMAVLCPTERSAQQLQDVMTLRGVPFATCFDREGKKTYS
                     RRKDVVHLLMYQSSKGLEFPYVAVINASFVHSGAADESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEDFA"
     gene            complement(3013..4215)
                     /locus_tag="ACHOQV_004248"
     CDS             complement(3013..4215)
                     /locus_tag="ACHOQV_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9632530.1"
                     /translation="MILVCVILWGIYVAVRALINLNERFIDAVSSPAGIIGLFFGLLT
                     VFAILLRFFIYRRLRKKTAAFEQAVSELVQRERGFNETVNAVIARGIRQEKEQLARRR
                     EEFHTARQKASRAMQRIVDSAWKFKAKTLLAGVTINNWQSKYDQLRKEREAYAAVSEK
                     IAFLNLEDNSDWESVRQQFLDKVALLEKAQEEKEYQAELKRQIREEKERQDELDRRQR
                     EAEEEERRLAEQQKLIEEALRAAEGAHREELEKQRLELEQKIQEAHAQYERAKSMAQL
                     TKQGHVYIISNIGSFGEDVFKIGMTRRLEPMDRVKELSGASVPFDFDVHAMISCDDAP
                     ALEKTLHDSLEKYRINRINLRKEFFRVKLEKIINEVERHHGQMEYVADPAALQYLQSL
                     EYAENEAA"
     gene            complement(4412..4714)
                     /locus_tag="ACHOQV_004249"
     CDS             complement(4412..4714)
                     /locus_tag="ACHOQV_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632531.1"
                     /translation="MSHCTSDGRFVCNDGSLSQSKRFCSGYGVAPAKRQVKSPVTTKL
                     VQKKKSAAVRKVSQPSVAGKNEVVPVTEPRKPTCAPLYMASKPGFTHLPICSGNQY"
     gene            complement(5061..6065)
                     /locus_tag="ACHOQV_004250"
     CDS             complement(5061..6065)
                     /locus_tag="ACHOQV_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9632532.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKFGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARRISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEPLQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPTGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQPGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFMMRLERQHGKLAEWVREQLNKKHSNVVACALANKLARIAWVITTQQKEYQA
                     "
     gene            complement(6323..6754)
                     /gene="tssE"
                     /locus_tag="ACHOQV_004251"
     CDS             complement(6323..6754)
                     /gene="tssE"
                     /locus_tag="ACHOQV_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP9632533.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSAEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTTVLQGMPGTAHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFIPEGRVLLRHLKQQRYVQT"
     gene            complement(6757..7293)
                     /gene="tssJ"
                     /locus_tag="ACHOQV_004252"
     CDS             complement(6757..7293)
                     /gene="tssJ"
                     /locus_tag="ACHOQV_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP9632534.1"
                     /translation="MAIIAGKAGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRNFDSADYQALFTGDSEILAGD
                     IIAQKDVWMQPGGSVTVDMPLDDAAKFTGVAAMFLEPDRKKNTWRVVLSRDELEPDTP
                     RLIEVSGNTLTLLPVKDK"
     gene            complement(7274..8374)
                     /gene="tssG"
                     /locus_tag="ACHOQV_004253"
     CDS             complement(7274..8374)
                     /gene="tssG"
                     /locus_tag="ACHOQV_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP9632535.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYTGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRTAEGLTSLVTLLAPGTQARVWHHDRR
                     RIPLKTPLAMRVHHPVSLKSRPVMGDHATDVNGQVLLQLSTQTGSEVQGWLPGGQLYS
                     DLLALLHVYLGSRLDVRLQLCVERSLLPDVRLSCRPAAGSPQLGRTAVMRTQAKITTS
                     AARVMTIRLGRYQRVQEHYQRKEAQENGDYRW"
     gene            complement(8329..>9647)
                     /gene="tssF"
                     /locus_tag="ACHOQV_004254"
     CDS             complement(8329..>9647)
                     /gene="tssF"
                     /locus_tag="ACHOQV_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP9632536.1"
                     /translation="PDGRSVLKIGFACSELADWSQVDLHRLSLYLAAEAPVSSTLHLM
                     MTKRLAALYLRLPGNDERIRIDGWFSPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFRE
                     KFMFVHLNGLENISLPAGISGFDIEVVLSQSWPADLPVTDDALCLHCVPVINLFTLEA
                     DPLIINGLESEYLLRPKRLQDGYTEIYSVDAVAGSGRTGSAEYVPFTSFRHRGGMLRH
                     DAPERYYHTRVKRGVTGMYDTWLILGGQRWEADRMPERETLSLRITGTNGQLPRRALQ
                     STLLDRCEQVLQTPISVCNLCKPTLPVYPPTEDRFHWRVMSHLGTGFLNMLSSAEVLR
                     GTLALYNWRDDELNHRRLDAILAVQHHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDI
                     HLFGEMLNRFLALYADMNQFNQLTLIVQPEGKCIRWKENHNPRLPG"
BASE COUNT         2423 a         2644 c         2184 g         2396 t
ORIGIN      
        1 gggtctgatg ttcctgcaca tccggagcat cagaccggag tgcctgagcc ggaagcgtta
       61 ccggagacga cgcttcgccc tgcacaggct gattttccag gcgggtgata tatcccttca
      121 ccatggcttc cggtaaagcc actttactgc cctgtttaag ttcgtgccgg gcaagtgtgt
      181 cactcagtgc cttcaggaat gtttcggtct gatacagcag gggcaggttg tcacggtcgt
      241 ccggaggcat ggcccgaagc gttttttgca gacggttgaa cagaccggta ataatttcca
      301 gccgggcgtg ggtattcagc ggccacatca ctgaccagtg atgccgggtc agcgccacaa
      361 tcagagccag cccttcattc agtccactta accctgctgt atgcatccgg gcgatggtgt
      421 accaggatgc cgtctgtaat tccacgccat tgtgttcata cagacgcagg cagagcgttt
      481 ccacgtaacg ccagtccaca tccggacgtg ccgggtgggt cagtttgctg atttcatcac
      541 gcagggcagc ataatccggt aatgacctgg ggtcaccacc ggttttcagt ttcacttcag
      601 aagtcatcgg cagattcggt gttactgtat taaagcatga ttaacagtga cgaaaagaat
      661 gtcagtgtaa taccgggacc cgggaaaggt attccacggc acggatgatt gccggtgtca
      721 catcccaccg ccgtttcaga accggtaaca gctcattaag gtttctgata taacgttcag
      781 tgaacagccg ttgtccgtcc ctggttaaat gaaaaccgga ctgattacag gtaatggtga
      841 tatgacaggt atcgctgcca atatagacag cttcctgggg tctgaatctg acattccagt
      901 gccaggagta cggagagtgg ttcagataaa aaagacgcat ctgaatatca ttaaataacg
      961 ggttcatggc gaattaaata aacctgataa taacagtatg atgaaagtcc ggaataaaga
     1021 aataatccgg tattatgcaa aatcctcaag gtgacgactg atactgtttt cacggtaaca
     1081 ggtcaccagt aattcacggg ttgcccgggt gaacgccaca taaagcaccg gaatcacttc
     1141 agactcatcc gctgcaccag agtgaacgaa agacgcattg ataaccgcca catacgggaa
     1201 ctccagcccc ttgctgctct ggtacatcag taaatgcacc acatccttac gtcgcgagta
     1261 tgtcttcttg ccttccctgt caaaacaggt ggcaaaaggc accccgcgta gtgtcataac
     1321 gtcctgcaat tgttgcgctg aacgctctgt cggacacagc acagccatat cactccagtg
     1381 accacacagg gcatggcgct tctccagcca gtctgcaact tgtctggctt catcacgttc
     1441 agaagcacaa cgcaggattt ccggctccgt accctcttca ccgcccgcct gtggctgaac
     1501 gagcggtatt tcacggttct gatgtttttc aaaatactcg cgcgagaatg cataagcaaa
     1561 gtgcagaatg cgtttcggat tacgataatt gaccggcagg acagaagtgc gtccctgtgc
     1621 ctgaatcccc acactggcca gagaaaagtt cagggcccgc tcccgccggt aaatggactg
     1681 ggcgtcatcg tacatcagga gcagtgaacg ggatgcgtta tcaaacagac gggcaatcag
     1741 ggccagccag cgactgtcaa aatcatgccc ctcatccacc agtacagcat catatccggt
     1801 tcgggtgatt tttccgctgt tcacggcctc ttccagtgca ctgaagcatt tatcaggata
     1861 ttcttttctg tcggccgtca ccttaatacc gtggcgtttt accattgagg cacaccagct
     1921 gtgaaaatgg gataccgtaa cccgtgacgc caaccctctg gcttcaatgc attcccgcag
     1981 atagctggcc agggtaatgt tgtagcacaa caccagaatc tttccgggtg tggtttcagc
     2041 caggtaaagg caacggaaca aaagaatcat ggtttttccg gaaccggcca caccatgaat
     2101 gacacgatgc ccgtcaccaa tatttctggc cagaatctcc tgctgaatat ccatgatttt
     2161 gatttgtgag ttctgtttca cggtcacttc aggaaacaga tgggcgcgca gaatatctct
     2221 catccggggt gtcacccggg ttctgaatcc cgtggtaaac atcccggcaa ttttttgctg
     2281 gaatacctca ggtaaaacag actgcgtcat ttcatcctgg cagattgtct gtgctgaagg
     2341 aaaaattttt tccaccgcat tctcatcatt ccctgccagt gtcttcagtt gctgacgggt
     2401 aatgcgggta aacaccacac catacgccca ggccaggttc agccgtccct tatactgacc
     2461 gtcattctgc cgtaagcacg ggtttgcagg taaggcatta accgtatcac aggcataacg
     2521 acgaacctgt accagcggat tgatttctga tttcagaaga ccgtcggttt ccagcgtgac
     2581 ctgctcctga tttacctggc gcagcgtgga aaccgtccag tccttgacct ccagaaatac
     2641 cagtccgttc tccgggtcaa taatgacaaa atccggatgc cggtttttcc gtcccaccgg
     2701 aatatcgtac cagacaaggc agtcatcatt cagaaagctc tccagtcgtg aagccaccct
     2761 cctctcccca ggcgtcattc tctcctcagt ggaggccgat acatttatca gtttcgccat
     2821 aaacgcacct taatctgtga acaaaaaata atcaccaccg acatccacgg atgttatatc
     2881 gtgggtgtcg ctttattcag aaataaaaaa cagaatcatt aacatgacca gaatactgtg
     2941 aaccatgctg cagggtatct gcagatatcc cttctgttat atccggccgg ggtaaaatca
     3001 gaacactgac tgtcaggctg cttcattttc tgcgtattcc agactctgca ggtactgcag
     3061 cgccgccggg tcagccacat attccatctg cccgtggtga cgctccactt cattaatgat
     3121 tttctcaagc ttcacgcgga aaaattcttt acgcaggtta atcctgttaa tgcggtattt
     3181 ttccagactg tcatgaagtg ttttttccag cgcaggcgca tcatcacagg aaatcatggc
     3241 gtgaacatca aaatcaaacg gaacagaagc accacttaac tctttgaccc tgtccatggg
     3301 ttccagccgt cgggtcatgc caattttaaa gacatcctca ccaaacgaac caatattcga
     3361 aatgatataa acatgcccct gtttcgtaag ctgagccatg gatttagcac gttcatactg
     3421 cgcatgtgct tcctgaattt tctgttccag ctccaggcgt tgtttctcta actcctccct
     3481 gtgagcccct tctgctgcgc gcagcgcctc ctcaatcagt ttctgctgct cagcaagacg
     3541 ccgttcttcc tcttccgctt cccgctggcg ccggtcaagt tcatcctgac gctctttttc
     3601 ttcacgtatc tgacgcttaa gctctgcctg atattccttc tcttcctgcg ctttttccag
     3661 caatgccact ttgtccagaa actgttgcct gacactctcc cagtcagaat tatcttccag
     3721 gttaagaaag gctattttct cactgacagc agcgtaagcc tctctttcct tacgaagctg
     3781 gtcatatttg ctttgccagt tattaatcgt tacaccagcc agcagagtct tcgccttaaa
     3841 tttccaggcg gaatcaacaa tccgttgcat tgcacggctg gccttctgtc tggcggtatg
     3901 aaattcctca cgacgtctgg ccagttgctc cttttcctgt cgtattcccc gggctatcac
     3961 ggcattgaca gtttcattaa aaccccgctc tctctgaacc agctctgaaa cggcctgctc
     4021 aaaggctgct gttttctttc tgagtctccg gtaaataaaa aaacgaagta aaattgcaaa
     4081 tacagtaagc aaaccaaaaa acaaaccaat aatacctgcc ggactcgata ctgcatcaat
     4141 aaagcgctca ttaagattta tcagcgccct gaccgccaca taaatccccc agagaataac
     4201 gcaaactaaa atcaggacaa atcccgctgc attatcatct tttttcttcc tgccatgact
     4261 ttcctttatt ttccttctca gtaactgaat atctttgatt aacgccctcc ggaaatattt
     4321 atacccggaa ggctgtccag ctttatgata cccgccagta cagaattaac tgcaggctga
     4381 gcacagattt ctctgctatt tcagtgtcag attaatactg attaccggaa caaatgggca
     4441 gatgtgtaaa tccaggtttg ctggccatat aaagcggcgc acaggtgggt ttacggggtt
     4501 cagtgaccgg tacaacctca tttttcccgg ccaccgacgg ttgtgacacc ttccggacag
     4561 ccgctgattt tttcttctga accagcttcg tggtgaccgg tgatttcacc tgacgtttgg
     4621 cgggtgccac gccataaccg gaacaaaagc gcttcgactg actcagactt ccgtcattgc
     4681 agacaaaacg tccgtctgac gtgcagtgtg aaataccacc tttagcaccc gagcagggtt
     4741 ttcttccccg cgcagcatct gtctgaaccg aaaataatct atggtcgccc ccgtttttgc
     4801 aacactgatt ttttaattaa tggtgggctt gcttaaatct atccggcgtc tctgtaggca
     4861 aggatgcctg cgccccgatg agtttcgcac ctgataatcc taaaaagccc ctcggcttca
     4921 gtgagccatt ttatttatta ggtttttaat caggtcgatt tgccgttcat gtcatcaata
     4981 atcagtcttc gcaaaaccag acaggcaact ttgtatctta ttgtttacaa taagccaatt
     5041 gagttatatc agcatttata tcaagcctga tattcttttt gctgagtagt gattacccag
     5101 gcaattcgag ccagtttatt tgccagagcg caggcaacca cattcgaatg cttcttgttg
     5161 agttgctccc gaacccattc ggcaagtttt ccatgctggc gttcaagccg catcatgaat
     5221 gagcgggcac actgaaccag taaccggcgc aaatttttat cgcctcgctt actgataccc
     5281 aacagagtcg attttcctcc cgtgctatac tgccggggaa cgagccctgt ggaagccgca
     5341 aaatctctgc tgcatgaaaa ctgtttcccg tcaccaggtt gcgacgatag taagctggca
     5401 gtaatcggcc caacaccagg aattgtcata atgcgctgag cggtatcatc agcgttgatt
     5461 gactgactta attctgattc cagctctgct atttgctcaa caaggtaaag gtagtgggta
     5521 tgcaacctca ccaaaaggcg gctcagatat tcggggattt cgtgttcagc caacacgaga
     5581 gacaaccgtt ttattacagc atcacctgtc ggcaggctga ttccaaattc cagcaagaaa
     5641 ccgtggatct ggttggttgt tttcacctta tctctgatga gtgattctcg gacccggtgt
     5701 aacgccctca tggcctgcag gggctcagtt cttggctgga caaaacgcat tgatggtcgt
     5761 gaagccgctt cgcatatggc ttctgcatca acaaaatcat tcttattact tttgacaaac
     5821 gggcgaacga actgtggaga aattagctta gtttcatgcc caaaatcaga gatacgacga
     5881 gccataaagt gtgcacccgc acaggcttcc attacaacaa ctgagggcgg acatgtcgcc
     5941 agaaactgca tcagtttagt acgggtaaat tttttacgca gaagagcctt accaaacttg
     6001 tcttgacaat gaatgtggaa ggagtgcttt ccgagatcga taccaataag tgtgatgttc
     6061 tgcatgatgg tctctccgtt aaagggattc accttaacag cgtactgctg ttaaggtggg
     6121 ggtgaccatc tcattagtcc cacgaacaga aaacagataa ttttcttcat ttaaaaactc
     6181 atacattgtt attgctgata tgccagagtt atcaggatga tgaaacattc cgccagcgtt
     6241 gtcataagcc ggaataatgt cacattcaca cctgacatat atccatcact gtttatttct
     6301 gttctgccct ccctgctccg gctcacgtct gaacgtaccg ctgctgtttc agatggcgca
     6361 acaacaccct gccctccggt ataaatgttg ttccgtaacg aaccagcccg gcatctttaa
     6421 gttctgcatc aatgacataa tgcaactcac caggctgagt ctgctcctgc atcgtcacgt
     6481 tgacacgctt aatacgtggc tcatacttca gcagaacatc tgaaagcaca cgcatcagtt
     6541 gatgcgccgt tcccggcatt ccctgcagaa cggttgtcat atccggcaga ccataatccg
     6601 gcagatgttt aaggcttccg gcccgggtat tcagaatacg ctgcatgtta tcgagcaccg
     6661 aaagaataac ctgctcttcc gcactgacct ggtttaactc cagcccgcca gcgaaattgc
     6721 catagagaat ttcataaagg gaaggacgcg gcatcactat ttatccttca ccggtaacag
     6781 cgtcagggta ttaccggata cttcaataag acggggcgtg tccggctcaa gctcatcccg
     6841 gctcagcacc acccgccagg tatttttctt ccggtcaggc tccagaaaca ttgctgccac
     6901 accggtaaat ttcgccgcat catccagagg catatccacg gttactgagc cacctggctg
     6961 catccataca tctttctgtg caatgatatc gcctgccaga atttcactgt cccccgtaaa
     7021 aagcgcctga taatccgcgc tgtcaaaatt ccggttgtct ttaagctgat aaatccgcac
     7081 cacaaccgac aggggaatac cggcagcact ggtgttaatg gcctccctgg cacgaatatc
     7141 aaggtgaagt gtttttatct gtcggtaaaa cagggatttc gtggcagata ccgtaccgtc
     7201 tgccactctt tgcgtcaggc cacaccctga taacgacagg gaaaacaacg caataatcag
     7261 accgtaacca gccttaccag cgataatcgc cattttcctg agcctcctta cgttgataat
     7321 gttcctgaac ccgctgataa cgccccagac ggatggtcat tacgcgtgcc gctgaagtcg
     7381 ttatctttgc ctgtgttctc attacagccg tgcgccccag ttgcggacta ccggctgccg
     7441 gtcggcaact gagccggaca tccggcaaca gtgaacgctc aacacaaagt tgcagacgta
     7501 catccagacg gcttcccaga taaacgtgca acaacgccag caaatcagaa tacagctgcc
     7561 cgcccggtaa ccagccctgt acctcgctgc ccgtctgtgt tgaaagctgt aataacacct
     7621 gtccgttcac atccgtagca tgatcgccca tcaccgggcg actcttcaga ctgaccggat
     7681 gatgaacacg catggccagc ggtgttttca gcgggatccg tcgtcggtca tgatgccaga
     7741 cacgggcctg tgttccggga gccagcagcg ttaccagtga tgtcagccct tcagctgttc
     7801 ttccgggtaa caacatcagg ggcaataacg ccagaaaacg cgataccggc gtggcaatat
     7861 tctgtgcaca tccggggatc cccagtctgg ccagaccgag cagatactgg gatgttttat
     7921 cctgcccccc cgcctcaaag gtggccgggt acgaatattt gcgccagata cggtaatact
     7981 gggtgatcag acgatgactg aatatatcga gaaaatccgc tgcggcttca tggccttccc
     8041 gctgctgtgc aatatcactg atatagtgag ccggcaacgg agacgtgacc ccgtaaagtc
     8101 ccataaacgt cacatgtaca gtgggtggca gatgggattc ttcaggaatt acagcatctt
     8161 ttatttcact cgcaggaaac cccattcccg tgtatggaca aaagcgaacc gcttcctgac
     8221 gggcctgcca gccactgcca atgggaggct gatctggctg gctttgttcc agtaactgac
     8281 agaagcgata aaaattaata tacggaagct gtttccggta acgggtaatt aaccgggcag
     8341 gcgcggattg tgattttctt tccaccggat gcattttcct tcaggctgaa caatcagtgt
     8401 gagctgattg aactgattca tatcggcgta cagggccagg aaacggttga gcatttcgcc
     8461 aaacaggtga atatctccct ctccggcaaa cccgttgcca tcaagagtta cctccacatc
     8521 aagcccccgg agcagaaaac ccttttcaaa acgctgaata cgatgatgct gtacagccag
     8581 aatggcatca agccgacggt gattaagttc atcgtcccgc cagttataaa gcgccagcgt
     8641 gccacgcagc acttccgcac tgctcagcat gttaagaaat cctgtcccca gatgactcat
     8701 cacccgccag tgaaaacgat cttctgtcgg cggataaacc ggcagcgtgg gtttacaaag
     8761 attacataca ctgatcgggg tctgcagtac ctgttcacac cgatcaagca gcgtactctg
     8821 caacgcacga cggggaagct gaccattggt acctgtaata cgtaatgaaa gcgtttcccg
     8881 ctccggcatc cgatccgcct cccaccgctg accgccaagg ataagccatg tgtcatacat
     8941 ccccgtgaca ccccgcttca cccgggtatg ataataacgt tcgggtgcat catggcgcag
     9001 cattccccca cgatgacgga agctggtaaa aggcacgtac tctgcactgc cggttcgccc
     9061 ggagcccgcc accgcatcca ctgaatatat ctccgtatat ccatcctgca gacgcttcgg
     9121 gcgcagcaga tattcgctct ccagcccatt gatgatgagt ggatcggctt caagcgtaaa
     9181 aaggttaatc accggcacac aatgcagaca cagggcatca tctgtcacag gtaaatctgc
     9241 cggccaggac tgactcagca ccacctcaat atcaaatccg gaaatacctg ccggcaaaga
     9301 aatattctcc agtccgttca gatgaacaaa cataaatttt tcccgaaagg tgaaatattc
     9361 cagcaataac tgatacccac tgaaagcact gtcacctttc ggccacagcc gatcttcttc
     9421 cgcaaaacca ccgggtgaaa accatccgtc aatgcggatc cgctcatcat ttcccggcag
     9481 cctcagatac agcgcagcca gacgctttgt catcatcaga tgaagggtgc tgctgaccgg
     9541 ggcttctgct gccagataca ggctcagacg atgcaaatcg acctgagacc agtcagccag
     9601 ttcactgcag gcaaaaccta ttttcagcac tgagcgcccg tccggct
//
LOCUS       DAZKGF010000183         9476 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZKGF010000183 DAZKGF010000000
VERSION     DAZKGF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.183"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            204..455
                     /locus_tag="ACHOQV_004255"
     CDS             204..455
                     /locus_tag="ACHOQV_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9632537.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(486..680)
                     /locus_tag="ACHOQV_004256"
     CDS             complement(486..680)
                     /locus_tag="ACHOQV_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632538.1"
                     /translation="MTIAERLIQKGFDEGFKQGFKQGALEVAREIACRLRDIGWPPER
                     IQEATGLSGEELKKLFPDEQ"
     gene            complement(742..1692)
                     /locus_tag="ACHOQV_004257"
     CDS             complement(742..1692)
                     /locus_tag="ACHOQV_004257"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP9632539.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPASFVEPDLHQYASDILWSVKTTGGKDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQYKTLPLVIPVLFYHGERSPYPYSMNWLDCFEDPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQQGIQKGIQQGIQQGIQQGIQQGIQ
                     KGRMEEKQDIARQLQKTGMPSAQICQITGLSEAELKNITH"
     gene            complement(1689..2078)
                     /locus_tag="ACHOQV_004258"
     CDS             complement(1689..2078)
                     /locus_tag="ACHOQV_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFP9632540.1"
                     /translation="MKSCFDTDKDFQMSSDEVTFNADSVRSKLELLSEELMHRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDAERGYCCHG
                     FDRNCCPLGCGDLDDDCCDAADLMEEP"
     gene            complement(2268..2534)
                     /locus_tag="ACHOQV_004259"
     CDS             complement(2268..2534)
                     /locus_tag="ACHOQV_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632541.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSERKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(2627..3061)
                     /locus_tag="ACHOQV_004260"
     CDS             complement(2627..3061)
                     /locus_tag="ACHOQV_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFP9632542.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            complement(3791..4291)
                     /locus_tag="ACHOQV_004261"
     CDS             complement(3791..4291)
                     /locus_tag="ACHOQV_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9632543.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(4752..5348)
                     /locus_tag="ACHOQV_004262"
     CDS             complement(4752..5348)
                     /locus_tag="ACHOQV_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632544.1"
                     /translation="MMKSDEKYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVRAAQDWLCDRKLVDITDILR"
     gene            complement(5345..6064)
                     /locus_tag="ACHOQV_004263"
     CDS             complement(5345..6064)
                     /locus_tag="ACHOQV_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFP9632545.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGELCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENTI"
     gene            complement(6061..6495)
                     /gene="psiB"
                     /locus_tag="ACHOQV_004264"
     CDS             complement(6061..6495)
                     /gene="psiB"
                     /locus_tag="ACHOQV_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFP9632546.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(6550..8508)
                     /locus_tag="ACHOQV_004265"
     CDS             complement(6550..8508)
                     /locus_tag="ACHOQV_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP9632547.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEDVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGSCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQMDATEHTTNLADAA"
     gene            complement(8572..8805)
                     /locus_tag="ACHOQV_004266"
     CDS             complement(8572..8805)
                     /locus_tag="ACHOQV_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFP9632548.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDNGEHFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            complement(8863..9390)
                     /locus_tag="ACHOQV_004267"
     CDS             complement(8863..9390)
                     /locus_tag="ACHOQV_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9632549.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     KTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPLQNAQAQPQPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
BASE COUNT         2058 a         2810 c         2522 g         2086 t
ORIGIN      
        1 gaagaaaaac gacggcacaa caatggcatg tcgtggtcac tggtgcacca ctcccgcaca
       61 ggtgaaaaca gagaaaacaa cacgacatat acaatcaaaa aaaagtgaaa tttcttcact
      121 ttttcactct tttttatcgc gctgacttgg tgactatgat gataaaataa agtgaagttt
      181 tttcactttt tcaaaggtgt tttatgaccg gctgggaact gcgaatctgg cgaaaaagca
      241 tgctctggtc acgcgaaaaa gcggccagag aatttggcgt gacacagaga acatggcacg
      301 cctgggaaaa tgcggaacag gtggatgtca ccgtctggag gaccacccag gcactttcag
      361 tccgtgacct gctgcctcac atgcagggga tgcgtaaggc agatatcatc aggcgactgg
      421 aaaatgaact gggggaaacg gcagaggatg tgtgagcgtg attcacagcc tggctgaatg
      481 ccaggctact gctcatcagg aaacagcttt ttcagttctt caccggaaag tccggttgcc
      541 tcctgaatcc gttccggcgg ccagcctata tcccgaagcc ggcaggctat ttcccgcgct
      601 acttcaagag ccccttgttt aaaaccttgt ttaaaaccct catcgaaacc tttctgtata
      661 aggcgttctg caatcgtcat gctgtttgct ctgctcctgc tgacaaaaaa cccggcattt
      721 tgccgggctt tgtggttctc atcagtgagt gatgttcttc agttcagctt cggaaagtcc
      781 ggtgatctga cagatttgtg cagatggcat tcccgttttc tgaagctgac gcgcaatatc
      841 ctgcttctcc tccatgcggc ctttctgtat tccctgctgt attccctgct gtattccctg
      901 ctgtattccc tgctgtattc ctttctgtat tccttgctgc tcaagacgtt cggcaatagt
      961 catcagttta tcctcatgct gcggcagacg ctcagccagc gcccgcataa attccggtga
     1021 tacagagtcc cctgcgttaa cgatgtaatg aatcagaaca cgcacctgtt catctgaaat
     1081 ttccaccatg acctgcggga gtttgtccag cagctccatc atgtcacgat ggcggatatg
     1141 cttcatcagc agcgtcagtg cggccatccg gcgatggttc atgatttcat tgtcatcaac
     1201 gacagtgata tcaaccagcg gaaacggctt tgtgtatatt ttagccgcaa gcgccggatc
     1261 ctcaaaacag tccagccagt tcatgctgta cgggtacggg ctgcgctcac catgatagaa
     1321 cagcaccgga atcaccagtg gcaatgtttt gtactgctcc agatgtctct gcatcgccgc
     1381 cacactgtaa cgtaacatgc ggaaaggcat gtacagattt tcggtgctct ggtgctcgat
     1441 gagcgtataa acatagccgt ctttaccccc ggtggttttc acactccaga ggatatcgct
     1501 ggcgtactga tgcaggtccg gctcaacaaa ggaggcgggt tccagcttca gcgtggacag
     1561 gtcgcacaac tgccggtact ccgccggaag atgcagttcc agaaaatctc tggcgacgtc
     1621 ggggttcgcc aggaacgacc ggaacgcggc atcatgcggc gtgggcgttg tggtgttttt
     1681 cttcttactc acggttcctc catcaaatca gcagcatcgc agcaatcatc gtccaaatcc
     1741 ccacaaccaa gaggacagca attgcgatca aagccgtgac aacaatagcc acgttctgcg
     1801 tcacggcgta tttcttctgc aatctcttca gcggaaaggt aatcaaactc ctcacgggcc
     1861 agttccagga ccagcagatg ttgttctgtc ggcaatgtag ccacaaaatt atcaatagca
     1921 gcactcaaac gtcgctgata tagctcataa acaagaccac aacggtgcat tagttcttcg
     1981 gataatagtt ccagttttga gcgaacgctg tcagcattga aagtgacttc atcagatgac
     2041 atctgaaaat ctttgtcagt gtcaaaacaa gacttcattt ttatctcctg cccgtccggg
     2101 ccctgaatga aagagaacct gtttcccttc tctgtttttt atggaacagg aggaatccgg
     2161 taccatgcga catagacgtc atgatgtttt tcatcttgct cacggttcct cctggtaaag
     2221 tacctgaaca tatcctgcca cctgcacaga cagacggcag ggggtaatca gtggcagacc
     2281 cgttcaattt caagcgcacg gagcaggtgc ccccactcgc tgactttcat ggcggggata
     2341 cgcacccgct tccgctcact gcagcgtctg gcgatatgac cgcccatcgc caggacgtcc
     2401 gggttaatct catccggtgc cagttcctgg cgtctgaagc gggtatggcg gtgcaggggt
     2461 ttgttctccc gcaggcactc ttcacgcaga cgctttacat actcggcggt tgcgaagata
     2521 attgatggtc tcatggcata atctccatag ttagtgaatg agatgccgga cttccgtacg
     2581 gggaagcgcc ggcggagaaa ggcagggttt atcgccctgc tttttttcat tccggacagc
     2641 gacgcagtgc cagcgcgagc gctttcatct tccagccttc cggcgtgcgg tacatcacct
     2701 tccagtagcc gtcgatgaac acataatgga agcgcacctc ttctgtgctt tccgtggcaa
     2761 tagcctccat gaaagaacgg tactcacgcg cctcactgtc catccggaaa ccactgtcac
     2821 gtccgtaata agccgtcaca ccgtccaccg ggttctgaag cgtatgcccg gcgggtttgt
     2881 cgcaacgggg accgaggcaa cggatatcac cgtgttcaac cagggcttcg gcctgctgac
     2941 cggtggcata gtgggtatgc aggatgcgac cgttccaggt cggctcaccg tgcaggtggc
     3001 agtaaacggt acggaacacc ccgtcgcggc aaaaaacgga aatcgtgctg ctgactgtca
     3061 tgaaaacgtc tccttctgct gaatcaggtc gccgggccga ccggttgctc ccccggaggg
     3121 gcgaaagccc cttctgccgt ccacttcttc ggggtttctc cccgcagtgg acctttccga
     3181 tggggcagcg acagccacgg cacggggcag ttgtccgggg gcagacgcaa aagtttttga
     3241 cgcttgcggc aaaaagtttt gtggctggta catctccccc tggacggctg acacgtgttg
     3301 tggccgcttc agcccccgga aagtccacag cgggaaacag gccgcagggg tggcggcgga
     3361 agggcagaca actgcgccat caggcgcagt ttccttacac accgtcaggt gtgcagggag
     3421 cgcagatcac ggcgcgacag aggcggcgcg accggaacgg tcgctgcaag ccaggttttt
     3481 cagacatcgg accgtagcgg cagcccgaaa ccgcgccagc gggttcggcg gagactggcg
     3541 ggtgcgcgag cagctgcctg tcggagtcgg gcctgacgac cggatgccgc caggcagccg
     3601 ggagccgaag gtgtccccgc ctccggaaat cagaacaaca gaaacgacat aacagaaaaa
     3661 tgcctcactc catgacgggc aggaagggca gcgccgcagg cgcggccctc ctgcatttca
     3721 ggcaggcgta agcctgccag ggttttccgg gggcctggca ggccataccc ccgccggagc
     3781 ggggagtaaa tcaccgacgg aacacatgac cgtcaataaa ggtgaaggag tccaggaata
     3841 aatcccgcgc atacgcctca tagtcaaaat agagcgccac cgtctccggc acgtcagcca
     3901 gcaggccggt atcactgacg aactcaaccg caaaagcctc ctcactgtca gcctcgcccc
     3961 accaggcatc gcggaagctg tcaaaatccg tctcaccggt atcatccacc cagagacgat
     4021 aagcctcttc gcagccttca tcccgtgcct ggcggaagcc ttcaacccag gcccagttga
     4081 tatgacattc agaggccata ttccccggga atccctcata atcctgaaac atcagttcag
     4141 gatcggcttc atcctggtga agagcacggc aggcggcgaa aaagtcgcgc tcatcatcaa
     4201 acgtggtcag gtcaaaccag cgtccggcga tgcttccaca gttgtatttg tgccaggttc
     4261 caacgtatac agcaggtgca acaacagaca ttcccgaatc ctccatagtc acatgttctg
     4321 cggcaccggg cggtgccgtg cctccataaa agccgggcga taaggcgggg atgcacaggg
     4381 cgcaagggaa gccgcagcct gacgcacacg gctggcggga gttggagggt gagaccgcga
     4441 aatctgagcg ggtgaacgac cggaaaatca cggcagacgg gaaggaggga ccgggtgacc
     4501 gccgcgtgca ttttaccctt gcatccccgc cagccggcta agatggaggc acggcacagc
     4561 ccgcacacga cgctgaggcc acataactga aaacccacaa ggccatgccc cgcatggccg
     4621 ggtaaaagcg gagccgccgg cgacttcctt attccggcga gtgcagagga cacgccgcat
     4681 gacctccaga ccacccctga tatgaaacga tcatgatcgg cgtgtccctg cgaaaaatat
     4741 acatgcctgg ttcaccggag aatatctgtg atatccacaa gttttctgtc gcacagccag
     4801 tcctgcgccg cacgcactac gctactgtcc ggtttcaccg ctttcagaga agatgccagt
     4861 cgtttgatca gcatcaccag ttcccgtgtc tgtatgtccg gcattgtctc cggcggagca
     4921 gagggactct catttccgac gcccatccca tgcgcttcca gtgtggcacg gacaaaccag
     4981 gcagcatcga ccagttgctg gcgaatccag gccagatcgg catttctgtc acgggctgcc
     5041 attcgctcac ccgacagcgc cttgctgata tggctgcgca ccagattatc caggagttcc
     5101 tgcgcttctt ccggtgaaaa gccacctggt atctctgtgc gcaacagtcc tttcctgtac
     5161 agtagctgca gcagatcaat ctgcgaccgc actgcaacta tcagtacata acggacaata
     5221 ttcgcactgg cggtggtttc tgtatcattc agcagttcct ccggatcatt accaccggta
     5281 ttgcagagca acggcaacag aggtcgcatc cacacgggaa cctgatattt ttcgtctgat
     5341 ttcatcatat tgtgttctcc agtttgttcg ggatcatcca ctgctcaagt gcacgtacct
     5401 gtaccggtgc accacgaccg gcctcaccgg cttcaaaaat cagccgccag agtggttcct
     5461 cctgccagaa aaaccggtca aatgatgaca gtgacggaaa acgcccgcac cacccccaga
     5521 aaatcgtttc cagatcatgc tcctcaacaa caccagacca gcgggacaac cagcttccga
     5581 ccgtggcggg agtatgaaac gcaaggtcaa tctgcgcctg agcaaaacat gtctggtatt
     5641 ccagttcccg tcgttctgcc tcacgttccc gctggcggct gtatttctgc tctgtcagcg
     5701 tggttttctg acgacgccgc tcaccttcac gaaaaggaac ggacgggaac agacggtgcc
     5761 ccgcatcgga tggcagggga agaacacaga gttcaccaaa actttcaatc agtttatcca
     5821 cggcagccag ccacaggaga cggttgccat gaaattcctc cttcgtcagc gcaggagcaa
     5881 aaaagcggag atcggtcagc gaaatccggc gactgccatt caggcagcga aagaaagcgc
     5941 tggcataagg ccatgctgaa agtgtgcgtc cctgacgacg acgttgttcg gtgacagcca
     6001 cagcctgcat ggcggcctgt tgttctgcgc tgagggggat cagtgcccgt gagcgtgcgc
     6061 tcatgcctga cctccttctg ccatcaggat atggatgatg tcgttaacac tgtacccctg
     6121 cgcatcaagt gtcgcggcaa gcgccagcgt gtgactgata gcgacaggcg tgaagatatg
     6181 ttgcacctga acgacggcga aaggctgtcc accacaattc accagaacca gcatccagag
     6241 ctgagagacg tctcccggcg aacataatgc caggtggaaa cgttcgtggg ctggcgtgaa
     6301 acgaacctgg acggggaaaa agcccccgaa ctcactgcca tactcgccat tcatcatcca
     6361 gttagccggt gcgtccactt cacgcatcac ctcgtgagtg agattacggc gcatatcgct
     6421 tccggcagcc cggatatctt catattcctg tgcgttcatg gactgcaggg cattcagggt
     6481 cagttctgtt ttcatcacgg tatctccctt gctgccgccc cgtctccggc ggggcggtgt
     6541 ggttgttcat caggcggcat cagccaggtt agtggtgtgt tcagtggcat ccatctgtgg
     6601 acgtggtgta cacatccagc caggcaccca gcggttgtct ttcatatgga actctgcatg
     6661 ttcagccgca tcgcctttct tcattttctc cgcgtcccgc gcagcaccgg acagtccggc
     6721 atcattcagg gctgcgataa tctgcggctt tttcaggtgt ccgaagaagt ttgcttttgt
     6781 cggctgccac cagtcgcgca tgtgaaaacc gatggcgctc tccagcgtgt ccagcgggct
     6841 gcgtgacgtg tgaccacact cgcgggtctg aacgccgtgg atgctgcacg cggtgcagaa
     6901 actgagcagg gataacagca cctcctgact gagtgaaagg aacgtcgtca tgtcccgtga
     6961 ccatccctcc ggtaacaggg ccgcaagacg ggctttttct gccatcatcg ccatgaacgc
     7021 ggcaccttcc ttccctgacg gcgcatcgct ggtcagcgaa taatgtttac attccaggct
     7081 gatttgtgct ggtttactgt acgctccgct gccaaacaca ttcaggcaga gcgtccatgc
     7141 cagcagtgcc agagatttgt ccggttgctg catcagtgcc gcctggactg ccagcgtgcg
     7201 ttcggaagac attttcgtca gcagtggcag actgatttcc tccactgacg ctttctcctg
     7261 cacctgttcc gtgcggtcag cgtcggcagc cgcgtcatcc tcgctgcgca actgcacacc
     7321 acgctggaca cacacgttgc cataacgcca ggacaccacc acaccgcttc cggcacgcat
     7381 ctccggcgtc cacgccctga ccttcgccat gcagcgcatc agcttcattt ctgcttccag
     7441 caggtcagat tcctcacact ggttttccag cgcgtcgtag cgcgtcatca gttcgttcag
     7501 gcgttcttct tcagcttccg tcagcaccgc ttccggctcc ggcaggctgc ggtatgttcc
     7561 ggcatcctca cggcaggaac cgacaggctc catacgtccg gcgcaccatt cccagccttc
     7621 ggcttcccga aggtgttcag cgacagcctg gagtttttcc agcagggcgg catcgagcgc
     7681 cacgcagtcc acatagccac cctcgtcgtc gctgaacagg tcagtgcgca gttcatctgg
     7741 cgagaaggca tcagccccca cgaagcggaa tttactgttc cccgccaccg ccacttcact
     7801 ttcggtcacc agacgacgaa tggtctgcac ttccggttta ccgccccatc ctgactggca
     7861 ggcggcttca aacacctgca cctgacgcgc ggtgtcgttc tccagcgcca gcgcctgaca
     7921 gtgctctgtg gtgatgcggt cttctgccag cgcatcaagg atgacaggtg caaggtctgc
     7981 cagtttcagc attcgctgaa cgtggcgggg tgaatagccc agcaaatcac cgatttgtgc
     8041 aggtgttttg ccttcctgcg ccattgcgcg gaatccggca atctgttcgg cagggtgcat
     8101 atcccgacga tgaccgtttt cagtcattga tgcggcagtc gccagttcct gcggaataat
     8161 tttcacgcgg acaggccagt cagccggaat gatgccacgc tctgccagca tgttgagtgc
     8221 tgccagtcgg cgaccacctg cggcgacacc gtggcggtca cctggcaggg catgaacaac
     8281 cagattctgc agcaggccga cgcccttaat ggactcagcc agttcgctga cggactccgc
     8341 agaatacggc accgtgcgca cattcagcgg tgatttaatc agtgaagcca gcggaacgct
     8401 cacatcctcc gtctgcgcca gcagggcaga caggacagtt tcctgcgttt ttgcaggttt
     8461 acggctggat ttgcgctctg tttttgcctt agactctgta actgacatat atttctcctt
     8521 tgaccggggt ggttgtatgt ctgccggacg cctacccgtc cggcacctct cttacttaag
     8581 aatgccgaaa tccctgatga catggttcat ccagtacccg gcaccgtcct caaaattcca
     8641 ggtacgccag accattgcac cgttattacg gacaaccagg cgaaaatgtt ctccgttgtc
     8701 atcctcgata aagacgttcc ggtactgtgc ggcaacggct tcggcctgtt tgcgggtaaa
     8761 aggaccgtca ggcagtccct gaagtattct gaaatattcg ctcatcctca tatctccggt
     8821 gatgccccgc gcagggcggg gcggttatca cagtcaggcc agtcagaacg ggatatcgtc
     8881 tgaaaacccg taatcctccc cctccggcgg ttgcggctga ggctcgggct gcgcggcctt
     8941 acgtccacgg cctttcgttt tcgcgccacc ttttttcgtc gcgtcagcac tctgtggctg
     9001 accattctgc tgaggctgcg gttgtgcctg agcgttctgc agtggtgcac tccccagcat
     9061 ctgcatggtg cccgtggtct taacaagaat ttcagtgacg taacgggtga taccgttatc
     9121 ttcccagcta cgggttcgga gttgcccctc gatatagacc tgcgcgccct tgcgcagata
     9181 ttcaccagcc acttccgcga gcttgccgaa cagcaccacg cggtgccatt ccgtctgctc
     9241 ccgcatctcc cccgtctttt tgtcgcgcca gctttctgac gtggccacct gcaggtttgc
     9301 cactgcgccc ccgttgggga tgtaacggac ctccggatca ttgcccagac gcccgacgag
     9361 gatgaccttg ttgataccac gtgcactcat gagaacctcc tgactgaaag atgtgagata
     9421 aaccggatac cggagacggg gcgttcatgt gccccttcag aggagcgaaa gctcct
//
LOCUS       DAZKGF010000184         9475 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZKGF010000184 DAZKGF010000000
VERSION     DAZKGF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.184"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            104..535
                     /gene="osmC"
                     /locus_tag="ACHOQV_004268"
     CDS             104..535
                     /gene="osmC"
                     /locus_tag="ACHOQV_004268"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFP9632550.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(591..1517)
                     /locus_tag="ACHOQV_004269"
     CDS             complement(591..1517)
                     /locus_tag="ACHOQV_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9632551.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENRELTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLTTHGCEVRQSLTIREDEREIRCWRAL"
     gene            complement(1510..2496)
                     /locus_tag="ACHOQV_004270"
     CDS             complement(1510..2496)
                     /locus_tag="ACHOQV_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9632552.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLKINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGHISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPVLTAYGDNNHRCACWYPQQEVISV"
     gene            complement(2493..3389)
                     /gene="ddpC"
                     /locus_tag="ACHOQV_004271"
     CDS             complement(2493..3389)
                     /gene="ddpC"
                     /locus_tag="ACHOQV_004271"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFP9632553.1"
                     /translation="MMLSEETSAVRPPKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMMGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQTLVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(3386..4408)
                     /locus_tag="ACHOQV_004272"
     CDS             complement(3386..4408)
                     /locus_tag="ACHOQV_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9632554.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVDNIRQQLGLDQPLYVQFYRYVSDLFQGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(4410..5960)
                     /locus_tag="ACHOQV_004273"
     CDS             complement(4410..5960)
                     /locus_tag="ACHOQV_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9632555.1"
                     /translation="MKRSILFRPTLLAIVLATTMPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSHPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(5974..6555)
                     /gene="ddpX"
                     /locus_tag="ACHOQV_004274"
     CDS             complement(5974..6555)
                     /gene="ddpX"
                     /locus_tag="ACHOQV_004274"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFP9632556.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIP
                     SEWWHFELPLAASYPLLADQFTCFISPGTQHAS"
     gene            complement(6813..9212)
                     /gene="dosP"
                     /locus_tag="ACHOQV_004275"
                     /gene_synonym="pdeO"
     CDS             complement(6813..9212)
                     /gene="dosP"
                     /locus_tag="ACHOQV_004275"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFP9632557.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSMLPLK
                     I"
     gene            complement(9237..>9475)
                     /locus_tag="ACHOQV_004276"
     CDS             complement(9237..>9475)
                     /locus_tag="ACHOQV_004276"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9632558.1"
                     /translation="VSRSRTLCLVDFGLRFNHKGRHYFKAANGEDIALSLSIGAAMFN
                     GHPDYERLIQIADEALYIAKRRGRTRVELCKASL"
BASE COUNT         2313 a         2483 c         2413 g         2266 t
ORIGIN      
        1 ggaattatat tttcgccgga ttttattcgg attatcctgc ttatcctcgt gctgtttctc
       61 acgtagtcta taatttcctt tttaagccca caggagagca acaatgacaa tccataagaa
      121 aggtcaggca cactgggaag gcgatatcaa acgcgggaag ggaacagtat ctactgagag
      181 cggcgtgctg aaccaacagc cgtatggatt taacacgcgt tttgaaggcg aaaaaggaac
      241 caaccctgaa gaactgattg gcgcagcgca tgccgcatgt ttctcaatgg cgctttcatt
      301 aatgctgggg gaagcgggat tcacgccaac atcgattgat accaccgcag atgtgtcgct
      361 ggataaagtg gatgccggtt ttgcgattac taaaatcgca ctgaaaagcg aagttgcggt
      421 gccgggtatt gatgcctcca cctttgacgg cattatccag aaagcaaaag caggatgccc
      481 ggtttctcag gtactgaaag cggaaattac gctggattac cagttgaaat cgtaaagcgt
      541 tgccggatga cgcgtcaggc gtgtgattgc ctgatgcgtt gttagcgtct ctacagcgcc
      601 cgccagcacc ggatctcacg ttcgtcctcc cttattgtta atgattgccg gacttcacat
      661 ccgtgggttg tcagagggca gcgttcgcgg aaaaaacagc cttgtggcaa cgtgcggttt
      721 cctggtagat ccgttttacg taatgcccat tcttcctcca gcggtttatc aatggcgggg
      781 agggaatcca gcaataatcg ggtgtatgga tgtgccggag ctgtcagcac ctgttgcgcg
      841 tctcctagtt ccacaatctg tccgagatac atcaccgcca cccgatcgct catatgacgt
      901 atcaccgaga cattgtgtga aatcagcaca taagtcagct cgcgattttc ctgtagcgtt
      961 accagtaaat tgaggatctg cgcctgcacg gagatatcca gcgccgaggt tggttcatca
     1021 agcacaatca cgtcaggctg cgaagagagt gctctggcaa tggcgatacg ttgccgctgc
     1081 ccgccggaaa acgcatgagg caagcggtcg agatactccg gacgaatacc cacctgcaca
     1141 gccagctcct ctgccaacgc tcgccgctgt tgttcactac tacgcttagc aatccagagc
     1201 ggttccgtga tgatgcgcca caccggtaag cgcggattaa gcgaagagag cgggtcctga
     1261 aacaccatct gcataatgcg ttgtgatcct gaacggatgt actgcccgtg gctcggttgc
     1321 agcataccca ttaaaagctg tgcgagggtg cttttgccgc agcctgactc accgacgatc
     1381 cctaaggttt caccacgacg gatctgtaaa tcaataccat tgatggcatg aacatgttcc
     1441 gtagttttac caagccagtt tttacgggcc gggaaattga tatggacgtc gcgtaacgtt
     1501 aataatgtat cagacactaa tgacctcctg ctgcggatac caacaggcgc agcggtggtt
     1561 gttgtcacca tacgctgtta gcaccgggac gttttcacac tgtgcacctg ccgcatagca
     1621 acgatcgcga aaagcgcagc cgtcaggcaa atgggtgagg tttggtaccg tccccggaat
     1681 agcgggtaat ggctggcgcg gtattccatg ttccggtgcg cattgcagca aaccaatggt
     1741 atacggatgc cggggatgat ggataacgtc tgccgtcacg ccgctctcaa tcacgcttcc
     1801 ggcatacatc acgtaaacgc tatcgcacag ttgcgacacc acggccatat catggctgat
     1861 gaacagtacc gcagttccac tggcgcgggc tttatgttta agcagacgca gtacctgcaa
     1921 ctgtaccgtg acgtccagcg ccgtagtcgg ttcgtcggca ataatcaatt gcggctcgca
     1981 ggagaatgcc agcgcaatca ttacccgctg gcgcatacca cctgaaagct caaacggata
     2041 gcgcgacata acttccacgg catccgggat ttgcatctct tccagcaggg caatcgcttt
     2101 agctctggct tcccgacgac ttattggttg atgatggcgg atcacgtcca tcatctgaag
     2161 acctattcga cgtgtcggat taagggcggt catcggttcc tgaaagatca tcgccactcg
     2221 tgcgccgcgc cactgacgaa gctgcttttc ccgagcgtta agaacatctt ctcccagcag
     2281 tgaaatatgt ccccgatgta cgcaataact gcccgtcggt agcagacgca taatcaacat
     2341 tgcagtgact gatttacccg agccggattc tcccaccaga ccgacaattt caccgcggtt
     2401 gattttcaat gacacattgt tgagcgcgtg aacgtcgccg ttaaaaccgg ggaaactcaa
     2461 atgcagttgt tgaatgtcca gaacgggttg ggtcatgact gctttcctcc tgctttcggg
     2521 tcaagcagat cgcgaatacc atcgccaaag agattaaacc cgacggcggt aagcaaaatc
     2581 gctgcccccg gaaatgcgca ataccaccat tgatcgagca catagttgcg accattcgcc
     2641 accatcgccc cccattcagc actcggttgt tgagcaccaa ggccaataaa tcccaacgtg
     2701 gcggccatta aaatcgcgct accgatatcc agcgatgcct gcacaatcag cggcggcagg
     2761 gagttacgta aaatatgcca gttgatcaga tgccagcgtg acgcaccaaa ggttttcgcc
     2821 gcctgaacat aggtatactg gcgaacaact aatgtttgcc cccgcgccag gcgcacataa
     2881 aagggaattc gcacaatagc aatcgccagc atggcgttaa acaaactcgg cccgagagcg
     2941 gctgccagtg ccattgtcag caccagcgaa ggaatcgaca gcataatgtc catgatgcgc
     3001 atgataatcg cgtctgcgcg tccgccaagc acaccggata gacacccgag tagcgacccc
     3061 atcatacctg caatggcgac caccactaat ccggcgagaa ttgattgctg actgccgacc
     3121 agtacgcggc tgaacagatc gcgtcccact tcatcggtgc caaaccagtg cgctgcagaa
     3181 ggcggcaaaa ggcgggcggt taaatcaatg gcgttgggat catgcgtcgc cagccacggt
     3241 gaaaaaatca tcatcaatag cattaataca atgatgactg cgccggtcac ggtaagcggg
     3301 ctgcctttca gcatccatac cagttttgca ccgttaaatc gcgtttgttt tggtgggcgc
     3361 acggctgacg tttcctcgct tagcatcatt caccacctcc acgtccgata cgcggatcaa
     3421 tccacaaata gagcaaatcc accaccaggt tcaccagcac ataagcaaac gaaaccacga
     3481 cggcaaagcc catcactgcc gggaagtcga gcgcctgtat tgatgttact acccatgcac
     3541 ccattccggg ccaggcaaaa acggtttcgg tgagcactgc gccatacaac aaatcgccca
     3601 gcgccagtcc caatacggta atcgagggga tcaacgcatt gggtagcgca taacataaaa
     3661 cgatatacca gccgggcaag ccgctggccc gggcggtacg aatgtaatct tcactcaatt
     3721 gttccagcat agctgagcgg atctggcgag cgacaattcc caggtgaacg aacgccagcg
     3781 ttaatgccgg taagatgaga tgttgcagcg cattgaagaa gacttcaccg ttgccttcaa
     3841 gcagcgcatc gagcagataa aagccggtaa cgtgcgttgg tggatccagc cagtcatcaa
     3901 gccttccgcc gccgggaaga atttgcagat gaccataaaa cagcacaatg acgcccagcc
     3961 caagccagaa cgcaggtgtg gagattccgg taatggccat tattcgcacc agatgatcca
     4021 gccagcgatt tcgccagact gcagagagta tgcccagcgg gatgccaatc agaagtgcca
     4081 gcagcagggc acaaaaagcc agttccagcg tcgccgggaa aaatatgcgc aactcttcca
     4141 gcaccggacg cccggtacga atggatgttc ccaggtcacc ctgaaacagg tcgctgacgt
     4201 agcggtaaaa ctgtacgtac agtggctggt ccagtcccag ttgctggcga atattatcca
     4261 cgatagcatc gctggcgcgg tcacccgcca gtaaccgcgc cggatcgccg gggatcaggt
     4321 gcgagataat aaacgttatc acacagacgc ccgccaccac gagcaccagc ccccagcagc
     4381 gttggcgtaa aatactccag aaggtcacgt tatttactca tggtattgat attgaagacc
     4441 tgttccagca tgggattgaa cacaaagcct ttcacctctt tgttcatcgc cagttggtag
     4501 tttttctgga acaggtacac ataagcagcg tcatcaatga cgattttctg tgcctgctgg
     4561 tagtcccggg tacgctgcgt ctggtcggtg gtcgctagcg cattgcgcag tagcttatcg
     4621 acctcactgt tttcatagaa cgagcggtta cccggcagac cttttttgtc tgactcaaac
     4681 cagtaattca taaacatata cgggtcggca aaatccggac tccagttgcc aatcgcgatg
     4741 tcgtaatcac ctttacccac tctgtcgcgc atggtggcgt tcgccagctt ttccagcttc
     4801 acattgatgc ccagtttgtt gagactggat tgtgtcgcca gagcgatcgg ttcccagttc
     4861 ggatcattat cagaatagag aaacgtcagg ctggtgggtt tgctcgtcac tttatcccat
     4921 tcggctttgg ctttcgtttc gtcatggttg tattgcattg ccgtcgcatc gtatccccac
     4981 atgccttccg gaatcgggcc gcgcatttgt tttccgttac cactcagaat gccgttaacc
     5041 atgccctgat aatcggtaga ccaggaaatg gcccgacgca gatccgcctg attaagtggc
     5101 gctttgctgt tattcagata cagataggtg acgcgcagtg acggatactc tgccacattg
     5161 actttatttt cctgcttcag ggcgttgagt tgatccaccg gcagcgcatc ggcaatgtca
     5221 atgtcgccac gggagagctg caggcgacgg gaggcacttt caccgataat ttttaccgat
     5281 actcgtttaa agttcggttt attgccgggg taatgcggat ttggcaccag aactaattgc
     5341 tgaccttttt gccagctttt cagcataaac ggtccggagc cagcggtatt ttgcgcgagg
     5401 aaaccacggg catcatccgc cgcatgctcc tttaagaccg ccggattgat aatggatgca
     5461 ccgtcattcg ccagcgtgta gaggaacggt gcgaatgggt ggctaagggt aaacttcacc
     5521 gtatgttcgt cgggagcatc aatctttaaa tctttgggaa atgcttctgc tggcccctgg
     5581 ccgattttta gtaaccgctc aaaagagagt tttacggctt cggcagtgac aggtgtgcca
     5641 tcggcaaatt tagcattgtc cttcagggtg aacgtccact ctttttgatc gtcagacgct
     5701 ttccagctac tagccagatc gccttcaacg tcggttgagc ctttatcacc gtccgttttg
     5761 tactgaacca gtcgctgata agacgggtag gtcactgtcc agtcgttatt atcaattgtc
     5821 accgctgggt cgagggtttg tgggtcggca gctttaccga tcaccagcat atctttcggt
     5881 acggcggcgt gcgcaaccgg cattgttgtg gcaaggacga tcgcgagcaa tgtaggacga
     5941 aacaaaatcg atctcttcat gacagtttcc tgattagctg gcgtgctgtg tgccagggga
     6001 tataaaacag gtgaattgat cagcgagtag tgggtaactc gctgccaggg gtaattcgaa
     6061 gtgccaccat tcgctcggga taccgacaaa gccaccgcca gtcataatcg cattcagcaa
     6121 caggcgattg cgctgagcgg cgggcgggac ggaagggtga taagcatggg aacgttcgtg
     6181 catttcatcg aaccctgcac ccatatcgag gatgttcccg tgctcatcac gaagcgtcag
     6241 gtcgatcgcc gtgccacggc tgtgattaga accgaccgtc acatcaacaa catattgcgg
     6301 gtctgggcag gcttgccaca acatcgcctg tgcttgttgt gggcgatacg catcgtaaat
     6361 caccagttgt aagcctgaca gctgggcgat actgatactt ttcgccagcg cagtaatcgc
     6421 atccttgtgt aacagacaac gcgcttgctg ataaatagct ttacctgtga tgttatcagc
     6481 gcaggcgtat ttcaattcga tctccagatc agggaagatc acggctaaat caaccagttc
     6541 ggtggtatcc gacataaacg attcctgtat aagacaaaaa ttgctgcgct ttccgcttat
     6601 gcagatctca tgccacgccg ggataagcgc cagaatgctg gcttaaagtt atatttttta
     6661 acttttgatc aacatttgtg cagcgtagtg cagttttggt gcaagagagg aagttaagga
     6721 aggaatctcc cggaatcgta gctgaaatca cagtatttaa gtgacagtgt cacgttaaat
     6781 gaaaacccgc gtgtgcgggc gagaggaatt tgtcagattt tcagcggtaa catactgctc
     6841 atccagcctg gaatttcttc ggcgggcagg gggcgggaaa agaaatatcc ctgaataacg
     6901 cgacagtgga tcttgcgtag catctcaaat tgctctttgg tttcgacgcc ttccgccacg
     6961 acggttaaat taaggctttg cccaatgctg gtaatggctt caagtaaggc aaggatgcgt
     7021 ttttcggtca gacaacgatc gacaaaactt ttgtcaattt tgatttccgt taccggaaga
     7081 ctgactaagc gggataatcc ggaaaagccc gtgccaaaat catctaccga taaaccgatg
     7141 cccatatcgc gcaggatctg aatgcgttta aagatttcgg tatcgtgttc catcatcatg
     7201 ctttccgtga tttccaccgt cagctggtgg ccgtcgatac cccaggcttg cattgcatca
     7261 gacacctgat taggcagctg attactgcga aagtgcagcg ccgacaagtt aacggataac
     7321 gccgggatat gaatattctg gctacgccat tctgctaact gacggcaagc ttccgcgatg
     7381 acccagcgcc caatattttc gatttcaccg atctcttctg cgagaggaat aaaccgtgaa
     7441 gggggcacat gaccatgcag gggatcgtgc cagcgagcaa gggcttcgat gccgtacagt
     7501 tcacccgttt ctgcgaagat ttgcggctgg taaaccagtt tcagttggtt attgctaatt
     7561 gcttctttca gcgccgcgcc taaaaccaaa cgctctttta ccatttcgtt catcgccggg
     7621 ctgaagaact gccagccgtt accgccattt ttgcgaataa aatccattgc attgtgagca
     7681 gtggagagca ggtaatcgcg gtttttaccc acgtcgtagc tgatgccaat gctcaaggtg
     7741 agcgggaatg gtttatcgtc aatcattatc ggcttgctga ccacattccg tagctcatcg
     7801 gcgatctgcg taatgttact gacgtcgttt tccaggctca cgaggacaaa ctgcgtacct
     7861 tcgatacgac agagatattg atccggtttg agtttttcac gaaagcgatt gaccacttcc
     7921 agcaatgcct gatcggccca cgcatagcca aggctatcaa tcacatcctg aatatggtca
     7981 acaccgatga gatacaccac cggagagacg gctttgtcga ccaggtcatc gaggtaattg
     8041 tgcaggttat tgcgatttgg cagaccggtc atcggatcaa attggatgag ttgttcaata
     8101 tgctgacggc ttttttcctg ttccagcgcc agcgcggcca tatgctggct gatatctgcc
     8161 acgcgttcga taaaggcgct ggtttctgct cctgacgagg ttttaatttg caggatcccc
     8221 gcaggcgcac catcacgctg acgaatggtc gctgaccagc tttgcgcatt ttgaacttct
     8281 gcaccgtgcg aagatgacgc ccagtgtatc ggcatcccgt tgcgcagcgc gaacagcgaa
     8341 acatgcgatt cgttgagtac agattcgatg ttacgacaaa tgatttcccc catttcatga
     8401 aatggcgggc tgctgcacat ggcggcgaga atattgcctt caagctggcg gatttgcctt
     8461 tcttcggtga tatccgagaa tgtcattacc aggttctgca gatgcgcgag cacgtcataa
     8521 accgggctga tagaggcttt aatccagatt ttttcaccgg tgcgcgtcaa cagcagaaat
     8581 tcgtcctgat cgcgggcggt tttccatagc aactgctgta aacgaatacg gttatcagca
     8641 gggaattctg gaatgttcag gagtgtatcg ggctgcatac cgctggcttc gttaatgcag
     8701 taaccaaaca tttcggtaaa tgcgcgattg cactgcacaa tatggcgttc cggatcgagg
     8761 acaatcaccg gtcggtcgag atggtcaacg gcaataatca attgtcgggt ctgttctttt
     8821 tgcgccattt ctacgctggc atcccgtacc agcgccaggt aataaacttt cccctcggcg
     8881 ctcactttcg atagcgcaaa acgggtccag attttactgc cgtctttttt ctccagctgc
     8941 agctcccgac tcatcccctc gacgcgagct ttaccacctt cacggttgtg acgaatgtat
     9001 tgaggatgcg caggacgcaa atcccgcgga atcagcatat caatgttatt gccaatgact
     9061 tcttcacgtt tgtaccccca gagcttctct gcggcggggt tgaaaaacat cacttcatca
     9121 ttttcgttaa ttaacaccgc gcccatcata ttttgctcaa gggcggggaa aaaaatgcca
     9181 tcggcggcat tatccgcatc ggttagcttc atgattacct ctgcatcctg gcgcatctaa
     9241 agactggctt tacagagttc aacacgggtt ctacctcgtc ttttggcgat atacagagct
     9301 tcatcggcta tttgaatgag gcgctcatag tcaggatgac cattaaacat ggcggcaccg
     9361 atggaaagtg agagggcaat atcctcgccg tttgcggctt taaaataatg tcgaccttta
     9421 tggttaaacc gcaggccaaa atcaaccaaa cacaacgtgc gagatcgact aactg
//
LOCUS       DAZKGF010000185         9421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZKGF010000185 DAZKGF010000000
VERSION     DAZKGF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.185"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            311..1609
                     /gene="kgtP"
                     /locus_tag="ACHOQV_004277"
     CDS             311..1609
                     /gene="kgtP"
                     /locus_tag="ACHOQV_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFP9632559.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(1606..1929)
                     /locus_tag="ACHOQV_004278"
     CDS             complement(1606..1929)
                     /locus_tag="ACHOQV_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFP9632560.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(1973..3328)
                     /gene="pssA"
                     /locus_tag="ACHOQV_004279"
     CDS             complement(1973..3328)
                     /gene="pssA"
                     /locus_tag="ACHOQV_004279"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFP9632561.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3442..6102)
                     /gene="pat"
                     /locus_tag="ACHOQV_004280"
     CDS             complement(3442..6102)
                     /gene="pat"
                     /locus_tag="ACHOQV_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFP9632562.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKSNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6134..6832)
                     /gene="tapT"
                     /locus_tag="ACHOQV_004281"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6134..6832)
                     /gene="tapT"
                     /locus_tag="ACHOQV_004281"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFP9632563.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPETLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTEAATGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6901..7320)
                     /gene="trxC"
                     /locus_tag="ACHOQV_004282"
     CDS             complement(6901..7320)
                     /gene="trxC"
                     /locus_tag="ACHOQV_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFP9632564.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7527..8564
                     /locus_tag="ACHOQV_004283"
     CDS             7527..8564
                     /locus_tag="ACHOQV_004283"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP9632565.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVLQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8612..9301)
                     /gene="ung"
                     /locus_tag="ACHOQV_004284"
     CDS             complement(8612..9301)
                     /gene="ung"
                     /locus_tag="ACHOQV_004284"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9632566.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREDVV
                     FLLWGSHAQKKGAIIDKQRHHILKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
BASE COUNT         2278 a         2399 c         2463 g         2281 t
ORIGIN      
        1 aggctatccg tcaggatggc ctttttgcgc tggagcagaa taaatgccgg atgcgacgct
       61 ggcgcgtctt atactcccac ataagctaga ttcagcagcg aatacggctt ccccaacgtg
      121 cccacctcca tacgtgtcct ccttaccaga aatttatcct taagctcctc aataaccatt
      181 ttcctgctaa ctaaattcgt ggttaaggtt gcataatgat atgcaacaaa tgtataacat
      241 tttctttacc aatagaaata aacaaaagcg accgacaaaa gcatcggatt acggcaggag
      301 acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac
      361 tcgtcgccgc atttgggcaa ttgtgggggc ctcttcaggt aatctggtcg agtggttcga
      421 tttctatgtc tactcgttct gttcactcta cttcgcccac atcttcttcc cttccgggaa
      481 cacgacgact caactactgc aaacagcagg tgtttttgct gcgggattcc tgatgcgccc
      541 aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct
      601 gttatcggtg tgtatgatgt gtttcggatc gctggttatc gcctgcctcc caggttatga
      661 aactataggt acgtgggctc cggcattatt gcttctcgct cgtttatttc agggattatc
      721 cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg
      781 caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcgggcaac tgctagctct
      841 actggttgtc gtggttttac aacacaccat ggaagacgct gcacttagag agtggggatg
      901 gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt tacgtcgtca
      961 gttagatgaa acttcacaac aagaaacacg cgctttaaaa gaagctggat ctctgaaagg
     1021 attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct
     1081 ttgtttctat accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca
     1141 tgccaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc
     1201 acttattggc gcgctgtcgg ataagattgg tcgtcgtacc tcaatgttat gtttcggttc
     1261 tctggcagcc atttttaccg ttcctattct ctcagcattg cagaacgttt cctcgcctta
     1321 tgccgctttt ggtctggtga tgtgcgccct gctgatagtg agtttttata cttcaatcag
     1381 tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc
     1441 atatgcggtc gccaatgcta tatttggtgg gtcggcggag tacgtagcgt tgtcgctgaa
     1501 atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcatt
     1561 tctggtttca ctaacattgc accgtaaagg aaaagggatg cggctttagt gacgggtcag
     1621 ttgccagacg gtatagccgg tgcttgcacc ggcgatatcc caggcaaaat ccttccagct
     1681 ccagccgctc ccctcagggc ggctatccca aagctctttt gacgccccca gactgacaga
     1741 gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgcatattc
     1801 atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct
     1861 ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag
     1921 aatacgcaaa acgtggcctc caaaacaaac cccgtacagg acggggttgt gattacagga
     1981 tgcggctaat taatcggtcg atgcggatac ggcgcaaacg acggatgagt ttacgaacct
     2041 tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg gtggtatgct
     2101 cgcggatcag ctccagttct ttctctcgct gtggcgcaag ctccagttgc ggatcgtgga
     2161 tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta ttaccggtga
     2221 tcaacatcca cttatcatca acccacatcc cttttaggtg ataggtgttg tcgtcatctt
     2281 tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg ctcaggaaac
     2341 gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa ggttcatctt
     2401 ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc tttttccctt
     2461 cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag tatggcgtac
     2521 agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg ttcaacagac
     2581 tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg gcatcgccct
     2641 ggaaatgata agcggcatca cgcagctcct ggcggaacag gcgaatgtcg ttcttgattt
     2701 ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca ttcataatat
     2761 tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatt agatgataac
     2821 ggtcgtagcg atatttatcg tgctgatgca ggtaaacatc gttcaggctg gcaccgctat
     2881 aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaagg gcttcacggg
     2941 tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc gccatgcggc
     3001 agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt tgtgcacgat
     3061 gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc tcatacaacg
     3121 cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcaaca atgcaaatgc
     3181 gctgcttcgc gctggctatt ttttccagca acgtctcccg gaagtcggcg ggagcgtaaa
     3241 agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg tgttgttgat
     3301 gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt cattgaaggg
     3361 tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc gattaagtgg
     3421 tcaacatttc cagtacctta ctcatgattc ctcgcgctgg gcaagattta gcgtaagccc
     3481 aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg ccagcgccac
     3541 catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt gatctcgcgt
     3601 ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga gatccgagcg
     3661 aaccagcacg gcaaattcgg catcgatgtt atcagggtcg gagatcgcac gcgtgacgcc
     3721 gaggatctct tctgtttgat caatacgtcg taccgccaca aaagccattt cccgatcgta
     3781 gtcgatctgc gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct cgctaaagta
     3841 gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagct gtggctcatc
     3901 ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta cccattcttc
     3961 cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt caaacggcgc
     4021 gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca aaggatgaat
     4081 atccagacgc tgaatttccg ggcaatcgac aatcaagtta gaaacctgca ccagaagctg
     4141 gctcaagcct gcaacatcta atgggcgtag cgcactgcgc gcacgaatct ttttactttt
     4201 gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggta gtgcgacgac
     4261 ggcttgatct tcaggacgcc actccacacc gccttcgccc agcatgatca acggcccgaa
     4321 aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt tagccatact
     4381 ttgcaccaac aggccgtgga tccgcgcctg cggccaggcc atttttacgc gatcgaaaat
     4441 agcgtttgcc gcttgctgga cttcattagc tgtacgcagg tagagcatta cgccctgaac
     4501 ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat aaccaatctg
     4561 ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggtgggga gcgtgttcat
     4621 gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag ccccttcggc
     4681 aatcgcctgt tgcaacagaa gatgcgcttc tgcggtatta gaggtcagat tgctgggcaa
     4741 cgccggcgtt tcgcgtagtt gcttctgatt acgtcggtac tccaccatat gcataaaagc
     4801 agtgatggtt ccttccggcg tgcgataggt cggcagcccg gcttcactga ataatcgtcg
     4861 tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc agagaaacat acttgctgcg
     4921 gggatgatgc tttaccgctt caattaatgc ttgcgcactt tctgttgcgg gagcagcggc
     4981 gctgggcgaa tgaataacca tcagcgcgtc aaaatcctgg ctgtggagca gaatatccag
     5041 cgttttaata tagtgctcac tgctggcgtc atcgcgtaga tcgagcgggt tagagattgc
     5101 cacatgttct ggcagtgcat cgcgcagttt ctggcaggtt tcttcgctta gcgttgccag
     5161 cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag caccgttgct
     5221 gataatcatc agccggtcgc cacgcagcgg gcgcatatgg ctgagggttt ccaccgccga
     5281 aaacagctcg tgagtatcct gtacccgcaa caaaccggca cgctgaatag ccgcatccca
     5341 cgccggatcc attcctgccg tggtgttcaa cagacgttga gccgccgggc tacgtccgct
     5401 tttaatcacc agaatcggtt tgttgcgaga ggcactacgg gccgccgaaa caaagcgtcg
     5461 tgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt cgcgggccag
     5521 atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa taaagtagga
     5581 aaagcccatc tcacgctgtt gcgcccagtc gaggatggta ttggagacgg cagccgattg
     5641 cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg cattcagacc
     5701 ttgccaggga gccagtaacc ccagactgtt tggtcctagc aggcgcatgt tatggcgcag
     5761 ggcgcaggcg cggagatctt catgttgcga tgccggggcg gaaagaataa tgcaggtttt
     5821 acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat tggtacataa
     5881 aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca acacacccaa
     5941 caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc ccgccagcag
     6001 gttacgcatc atcaggtaac ctgctcgatt ggatttcatc gatgcgccaa ttaccgcaat
     6061 cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct cccgcagaag
     6121 tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc agatgttgcg
     6181 tttttcctgc cagatagcgc gttttaaaac gagtgaaatg ctcgcctaag cccgttgcgg
     6241 cttcggtatc gccggccata tctaacagtg cgatggctac ctcggcagta caatattggc
     6301 cttcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg acggaaatga
     6361 cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttca ggccaggtac
     6421 catcgagcat gataaatagc ggaggcttac cggcaggtgg tgtgaagatc acttcccgtt
     6481 gctcatcagc atacgaggcg ggaaagacca ccattggctg atagtccggg ttttgtacca
     6541 gatccagcaa atctagcgag ggttcggtac gcgaccattg aaacgcaacg gtatcaggca
     6601 aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg tcgaacatca
     6661 gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga cataatgtct
     6721 ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga aaaggacgtg
     6781 ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc ataagagagc
     6841 gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc cccggtaaga
     6901 ttaaagagat tcgttcagcc agctatcaaa cggcgctttc ggtactgcgc cattaagcat
     6961 gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa ttccaaagcg
     7021 actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt taccgctacg
     7081 ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc accacggtgc
     7141 ccagaagtcg atcaccacag gtagatcatc tttcagcaat ttgtcgagcg tttcaccggt
     7201 cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt ttgccgcatc
     7261 ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa cggtattcat
     7321 aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg tttctaatat
     7381 gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa taggtatgat
     7441 agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta atctgcgcgc
     7501 ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa agcggcaagg
     7561 tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac aacccgcgta
     7621 ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga cggtggccgt cgccccgccc
     7681 gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agagtcgccg tggcgcacag
     7741 tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc atcagtggta
     7801 aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc cgcgtctacg
     7861 gcgaaaatgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt cgcgcctggt
     7921 ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca gcaaaccgca
     7981 aagcgtacca tgtggtagat gaagcggaac tgacaaaagc gtcaggcacg gaacatcacg
     8041 gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgctgcag tgggtaagcc
     8101 aggcaggcgc gcaggattgt gtactggcgc tggaaaacga atctaacccg cataacctgg
     8161 gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg cgttgtggtg caggatgcgg
     8221 cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag cacgttcagc
     8281 cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca ggttacaccg
     8341 tagtgaccac ttccagcgag caggggaaac cgctgttcaa aaccagtctg ccagcgaaaa
     8401 tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc gatccgaacg
     8461 atttgcgcgt gaagattgat ggtactggca acgttgccgg gctaaatatc tctgttgcaa
     8521 ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc agccgggtgg
     8581 caacgctgcc atccggcgtt ttcctgcaaa tttactcact ttctgccggt aatactggca
     8641 tccagtcaat cggtttctcg ccacgttgtt ccagccactg atttgccagc acaaaatggt
     8701 tgcagccaaa gaacccacga tgcgcagaaa gcggtgatgg atgcggcgct ttcagtatgt
     8761 gatggcgttg cttatcaata atcgcccctt tcttttgcgc atgtgagccc cacaacaaaa
     8821 acaccacgtc ttcgcggtgc tggttaatca ggctgattac tttatcggtg aaggtctccc
     8881 agccgaggct ggcgtgggaa tgtgcctgac ctgcgcgaac cgtcaacaca gtattgagta
     8941 gcagaacgcc ctgacgcgcc cagctttcaa gataaccatg attagggcgg gtgaaaccgg
     9001 gaatagtatt ttccagctct ttatacatat tcagcagtga cggcggtgtc gcaatgccgg
     9061 ggcgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga tcctggccga
     9121 gaatcaccac tttaacgtca cccaactctg taaagcggaa cgcgttaaag acatcttttt
     9181 gtggcggata gatagtgacg ccggactgcc gctcgctggc gacggtctgt agggtattaa
     9241 gaaaataggg ttgctgcttc tcttcagcca gcacatcatg ccaggttaat tcgtttgcca
     9301 tctcgctctc ccgcgaatct tcaatccgcc tagcttaact gcttctttct acagaacaaa
     9361 atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt aaaatttcac
     9421 a
//
LOCUS       DAZKGF010000186         9206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZKGF010000186 DAZKGF010000000
VERSION     DAZKGF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.186"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2391
                     /locus_tag="ACHOQV_004285"
     CDS             <1..2391
                     /locus_tag="ACHOQV_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFP9632567.1"
                     /translation="STRDFSASEIENELRTHNVISSIYYEIKDIINSLGISQGNMEYY
                     ATIVKHSTTFRLKRHPKWQGILYLCCYLYFRYREINDKIITAFRYLVKKHKDASVLFA
                     KARATEEIDLINKKLKFASKIFNFFVDSNIEDSIPFGYVREKVFSLISKDDMHLVSEF
                     FDKNKIDVIEYQWQYIDSHHLKIANSIRKLFLAIDIVYDNEKSVIGQQITAAKDDLRG
                     KGKLDAKDLRVIHPSEKNYIVKEGEINTQRFEFHMYSRVSNHLENGVFYAAESEQNKR
                     LEDDLIERSYWHKNKSILIEKTGLERLITPITQTLNELENTFSDKLARITIGINSDAN
                     EFVKKQPHSNRLQWSLASKKWKTSIDNPIYNQIKNIGIVDVMKYVNNETNFLSVFNAI
                     SSRKNNLEADYNDLLACIFGNGANYGVHRMASASDRSIGILRDVNDKFIRPETTGMAN
                     TVISDAIANLSVYKYWTINEDSPFGSVDGQKHTCRINTFKARYSAKYFRKGKGVSAMT
                     LVSNHVPLSTVVISPNEYEGHFAFDLLYNNTSEIQPKSLATDNHGINNVNFAILDIFG
                     YQFSPRYAKFKKIFNELFDVILESDKLIIKLKKQFNFKLIEHEWENIQHIICSLSRKT
                     TIQSTVVKKLSNTKRSNKTLAALREYDRVIKCIYVLDYADNKTLRQFVQQALNRGEAY
                     HQLRRAIASVNGNQFRGGNDYQIDQWNDCARLIANCVIYYNSAILSRLVEKFEKENNK
                     KAIDVLANLSPVAWRHILLGGNYSFEDQVAITNLDSLLEGVDPLSDIGDIESEYDG"
     gene            complement(2546..2845)
                     /locus_tag="ACHOQV_004286"
     CDS             complement(2546..2845)
                     /locus_tag="ACHOQV_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9632568.1"
                     /translation="MSYEIEWRPGAIEDITALFEYLAENASLWDARNLTERLITATDR
                     LADYPRLYETDERYGEGVRRISLLGQHVLYEVNDEASRVNILAVVGQRQNPRFII"
     gene            complement(2835..3098)
                     /locus_tag="ACHOQV_004287"
     CDS             complement(2835..3098)
                     /locus_tag="ACHOQV_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFP9632569.1"
                     /translation="MNSTTHYENANFLRELAESLPRILPEGSTDKSALLQRLANEELA
                     RAEYDEQVRAKIAAARADKRPGISSAQLRQQLQGRYQELRNEL"
     gene            complement(4167..5024)
                     /gene="repA"
                     /locus_tag="ACHOQV_004288"
     CDS             complement(4167..5024)
                     /gene="repA"
                     /locus_tag="ACHOQV_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFP9632570.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALFSSLDI
                     SEEAVASARRSRVEWENKLRKKQGLDALGMDELIARAWRFVRERFRSYQAELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            complement(5017..5091)
                     /gene="tap"
                     /locus_tag="ACHOQV_004289"
     CDS             complement(5017..5091)
                     /gene="tap"
                     /locus_tag="ACHOQV_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFP9632571.1"
                     /translation="MPGKVQYFFLCSLLLRIVSAGWCD"
     gene            complement(5314..5577)
                     /gene="repA"
                     /locus_tag="ACHOQV_004290"
     CDS             complement(5314..5577)
                     /gene="repA"
                     /locus_tag="ACHOQV_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFP9632572.1"
                     /translation="MSDAVRSATSSEARPKRAYRKGAPLSVNERKRLSLNRKRDTHKA
                     LNVFIRNELKDILERFCQDKHLTLGEGIELLIQQEKERAKNGE"
     gene            complement(5981..9178)
                     /locus_tag="ACHOQV_004291"
     CDS             complement(5981..9178)
                     /locus_tag="ACHOQV_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006707074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFP9632573.1"
                     /translation="MIERKPHVHIIIPEVNLLSGNLINPRGDYRVNEKYFEAVQEYIN
                     QKYNLISPRERVRANPKNVSDILSRYKGDDFRSKNREFKEQLVRDIVEENILSRKDFY
                     THVSSFGDIRIRNQGKENEYIAVKLPGDKKFTNLKETIFNDDFIVRRELKKPPLEKSV
                     IQQLLHEWPQRAMELKYIDKANSDFRKKYYSSSQEEKIKILAQQETAFYQKYGERYDV
                     FPAEWKTNHKSGINEAQTRGTTFSPNGLQSMSGGEMATGWQTGNTGSSLFLSTDALVF
                     MGQSQSSGRDSGLRCDVHSGGRGRSGNQRGSGQPAITGTNATVHSRRSTRTQYGNRYA
                     GGLLAGDVSIPVYARNKPGGATIQDVIRRSEILLRPPPGKSNISVNRFNGDIPQMGKN
                     AAYVAAHFVRQYEQNKILPNQRQALWDIRQLYQSTRSFIWQDERLTRKERSQYLSVLA
                     FEHVKARQKIINSSQQENTMASEDIRKLIKKKPRLSNNEITGKDERKIPPAGKRFSRI
                     VHTLKNRIEGEDKENSYAKSVSAADLYTRKSKLSKNVHYLDKNSNRTLFVDTGNAIAI
                     RKDGMSPAALGIALELAKERFGSTLTIKGNEEFRNQVIDIVARNHPDLHFSDKAMNRQ
                     LEERRAEIAAERDGMTIEGVSKTSTSKEQEKEHEQAADTKNQTNVKESSEPHESPESR
                     TVVKLEGKLIDHGKAPLDNKKGAEQSYFVTVRGRDGKETTHWDSDLQNVMKGKRRGTE
                     ISLELKESNPVQVSTRDHLGNLSIREAVRQTWTLNKMSVGQEQKREQSLEKIQQNQSP
                     IPARTQTAVDLILANPVLRQMAMTAEQKEREFTALQADKESVREQTDQEIRNRLGSGD
                     QSREPLAVILSDDELRGMALSMEQKMREMATPVDKLDAVKAEINSEIAARVAWNSSIP
                     VPVSEVSATVAQKEIHREAESRGNKDKVHEGVLLDHGRAAYKFKPDISKPEDERDDSY
                     FVKLKMDNGKTRTLWGVGLESAVAGYSKGERLRLEDKGTEKVQWIERMKDGREIEKNG
                     DRRVWDCRSLTRESEVMKGFRVSGMPERDDGPDVA"
BASE COUNT         2472 a         2106 c         1934 g         2694 t
ORIGIN      
        1 agcacgagag acttctcagc atcagaaata gaaaatgaat taagaactca caatgtgata
       61 agtagtattt actatgaaat aaaagatata attaattcat taggtatatc ccagggaaac
      121 atggagtact atgcaactat cgtaaaacac agtacaacat tcagacttaa gcgacaccct
      181 aaatggcaag gaatacttta tttgtgctgc tacttgtact tcagatacag agagataaat
      241 gataaaataa tcacagcctt cagatattta gtaaaaaaac ataaagacgc ttcagttttg
      301 tttgcaaaag ctcgggcaac agaggaaata gatctaataa ataaaaagct taagtttgcg
      361 agtaaaatat ttaacttctt cgttgatagt aacattgaag acagcatccc ttttggatat
      421 gttcgtgaaa aggttttttc acttatatcg aaagatgata tgcatcttgt gagtgaattt
      481 tttgacaaaa ataaaatcga tgtaattgaa tatcaatggc agtatattga cagccatcac
      541 cttaagatcg ccaattcaat ccgaaagctt tttctggcta tagatatagt atatgacaac
      601 gagaagtctg ttattggcca gcaaattact gctgccaagg atgatctcag aggaaaagga
      661 aagctggatg ctaaggatct gagggtaatt catccatccg agaaaaatta cattgtaaaa
      721 gaaggagaaa ttaacactca acgattcgaa tttcatatgt acagcagggt atctaaccat
      781 ttggaaaacg gtgtctttta tgctgcggag agtgaacaaa acaagagact ggaagatgat
      841 ctcatagaac gaagttactg gcataagaat aaatcgattt tgatagagaa aacaggcctt
      901 gaacgactta tcacaccaat aacgcagact cttaatgagc tggagaatac ctttagcgat
      961 aaactggctc gtataaccat tggaataaac tcagatgcta atgagtttgt gaaaaaacaa
     1021 ccacactcca acagattaca gtggagtctg gccagtaaaa aatggaagac cagcatcgat
     1081 aatccgattt acaatcagat aaaaaatatc ggaatagttg acgtaatgaa gtatgtaaac
     1141 aatgagacga attttttgtc tgtgtttaat gccatatcat ccaggaaaaa taatcttgaa
     1201 gctgactata atgaccttct tgcctgtata tttggtaatg gcgcaaatta tggtgttcac
     1261 agaatggcat ctgcatcaga ccgtagtatt ggaatactac gtgatgtgaa tgacaaattc
     1321 attcgtccag aaacaacagg aatggccaac acagtaatat ctgatgcaat tgcaaatctt
     1381 tctgtatata aatactggac gataaatgaa gattctccat ttggaagtgt tgatgggcag
     1441 aagcacactt gcagaataaa tacatttaaa gcccgttatt ctgccaagta tttcagaaag
     1501 gggaaagggg tttctgcgat gacactggta tctaatcatg tgccattatc aactgtggta
     1561 atttctccaa atgaatatga aggtcatttt gcatttgact tgttatataa caacaccagt
     1621 gaaatacaac caaagtcatt agcgactgac aatcatggta taaacaacgt taattttgcg
     1681 attcttgaca tttttggcta tcagttttca ccacgttatg caaaattcaa gaaaatattt
     1741 aatgaattat ttgatgttat actggagagt gataaactca ttataaaact taaaaaacaa
     1801 tttaacttta agctcattga acatgaatgg gaaaacatcc aacatattat ttgttcttta
     1861 agcagaaaaa caaccattca aagtacggtt gttaagaagt tatctaatac aaagcgtagc
     1921 aacaagacat tagcagcact tcgggagtat gaccgagtta taaaatgcat atatgtcctg
     1981 gattatgctg acaataaaac cttaaggcaa tttgtgcaac aagcactgaa cagaggtgaa
     2041 gcttatcatc aactaagaag agcaatagca tcagtaaatg gaaatcaatt tcgcggtggt
     2101 aatgactacc aaatagatca atggaacgac tgtgcgaggt taatagctaa ttgtgtaatc
     2161 tattataatt cagcaatatt atccaggctg gtcgagaaat ttgaaaaaga aaataataaa
     2221 aaagcgatag atgttctggc taacttatcc ccagtagcat ggagacacat tctccttgga
     2281 gggaattatt cttttgaaga tcaagttgcg atcacaaatt tggacagtct tttagaggga
     2341 gtagacccac taagtgatat cggagatatt gagtccgaat atgacggtta acaacatgat
     2401 aaataatggt ttttatgcta agagtgagac ttttggagga aatgggcgca gaacccctct
     2461 tagcgtgaga aaatttcctt tctccaagcg gctcccaaac cgtaattcct ggcaagtccg
     2521 gtaagctgag caccaagccg ggcgctcaaa taatgaatcg gggattctga cgctggccga
     2581 caaccgccaa gatattgaca cgactggcct catcattcac ttcataaagt acatgctgtc
     2641 caagtaagct aatacggcga accccttcac catagcgttc atccgtttca tacagcctgg
     2701 ggtaatctgc caggcgatcg gttgcagtga tcagacgctc tgtcaagttc cttgcatccc
     2761 aaagagatgc attctcggcc agatattcaa acaacgccgt tatatcctca atcgcacctg
     2821 gtcgccattc tatttcataa ctcattgcgc agttcctgat aacgtccctg tagttgctgg
     2881 cgcagttgcg cggaacttat tcccggtcgc ttatcggcgc gagcagcggc aattttcgct
     2941 cgtacctgtt cgtcgtattc cgcccgtgcc agttcttcat tagcgaggcg ttgcaatagt
     3001 gcagacttgt ctgtactgcc ttctggcaaa atacgaggca ggctttcggc cagctcgcgc
     3061 aggaaattgg cgttttcgta atgtgtggtg ctattcatag ttgactcccc ccttcattca
     3121 aaaagggtaa ttgcagattt tttgataccg cacgcatgag tataacgagt cagagtatcg
     3181 atattcgccc cggtgatatt gtttcacagg caggcaataa ccggaaaaat aatgctcatt
     3241 tgcctggcaa tctgcgtgtt gtcaggtttt atgcagagta tataaccttt cagacacagc
     3301 caccaacata tgcttatgcc gcatcagcgg catggaaggc ggcactctgt tgtttcatat
     3361 gataccggag taaaaccgcc gaagcccggc gtaagccggt actgattaat agatttcacc
     3421 ttacccatcc ccaccctgca aagctatacc cgctgtcagc catgacagag cttatgtgcg
     3481 cggtcggagt ggtcccgacg agggtttacc cgaagtcggg gcgtatctcc gcgttagcgg
     3541 gccgtcaggg ccgcttacga gcgtgtactg agaacatcca gccagaagac tgacagcgat
     3601 gatggattgt agttacaaca ttcatgatta aaagcgatgt tgttcccgcc cttcgggcgg
     3661 gggcggcggc gcttttcagt tatgagggaa gggctttgtg gtttcagttc tgcgcgggac
     3721 cgggtttttt ccggtgcttg tatttgagtg ttgtaactaa agtggctccg gtcggggccc
     3781 gccgcttgcg gtgggaggtg catatctgtc tgtctacagg acaggcagtg aataggtttt
     3841 ctttttaaat ggaagtaatt aagtagttta aaggaaatac agtactgggg tttaaaagaa
     3901 acattgcagc ctgtaacgcc tgcggctggc gctttatgac atgaacgatt gtaaccttat
     3961 ggagaattcc cttgcagtta aatgtggata agcggaattc tccgtctgtg agacgtgttt
     4021 tgcgtgaaaa accgggggag cattgcgagc cggaaagcag atgatgaggc tgttttttat
     4081 gtgctgagct gcttttttgt gcgtcgccga ccttcagacg tgcggaagcc tccggcgcag
     4141 gccggattat tcagatgagg taaaactcag ggggaggctg tggccagccg tgtgtagttg
     4201 ttgttccgcg acattatcat gcgttccttc acgcgacggt cgatttcgcg cttcacggcc
     4261 tccaggcttc cgcggaaacg cccttccgcg atttcacgtg tcagctgccg tttcaccagg
     4321 gtgacaatgt cctgacgggt cctgcctgca tcgcggcgcg cacgggcacg tttcatgccg
     4381 tgggacttaa gctctgcctg ataactacgg aaacgctcgc gcacaaaacg ccaggctctc
     4441 gctatcagtt catccatacc cagcgcatcc agcccctgct ttttgcgcag cttgttttcc
     4501 cattccacgc ggctgcggcg tgcggaagcc accgcctctt ctgatatatc gagtgaggaa
     4561 aacagtgccg gcgtgaacgt gatgtcggtc gggatgttac agccaattgt cgggtcgtat
     4621 tccgtctggt aggttatcag ccccagctca gacaggaact tcagggcacg ggtggcacgg
     4681 gtgatggaga gtttcccggc ctcagactcc gtcgccagtc cgcactcaat ggccagcgtg
     4741 gtgatggagc actgcacgcg gttggccagc gggtcatagt ggaaacacag cccctgcagg
     4801 agcgcatcaa tagcccgtcg acgcagcacc ggtggcatgc gtcgacgcag accacgcgaa
     4861 cgggcatgcg ccacatgaat ggcgaaatca aaacgggagg tgaagcccac cgccttttcc
     4921 atcagttttt cgcagaactt cagcgttcct gcaccttcac ggggtgtgaa caccggattc
     4981 gggttcttta cctggcggta atacgtttgt tgaagatcag tcacaccatc ctgcacttac
     5041 aatgcgcaga aggagtgagc acagaaagaa gtattgaact tttccgggca tataactata
     5101 ctccccgcat agctgaattg ttggctatac ggttttgtag gcccccggta atcttctgcg
     5161 tcgccaaaca tagaaaggtt atcggggttt ttgcttttct ggctcctgta aatccacatc
     5221 agaaccagtt ccctgccacc ttacggcgtg gccagccatg aattttctgt ccgtaatcta
     5281 tcactctgcc agaaaaggat caaataaagg tgactactca ccatttttag ctcgttcctt
     5341 ctcctgctgg attagcagct cgatcccttc acccaaggtc aaatgcttat cctggcaaaa
     5401 gcgctcaaga atatctttca attcattacg gatgaaaaca ttaagcgctt tatgtgtatc
     5461 ccgctttcga ttgagagaca acctctttcg ctcattaacg cttaacggtg caccttttct
     5521 atatgcccgt ttaggtctgg cttcagagga agttgccgaa cgtacagcgt cagacataca
     5581 gcctcctcat agatcaaaaa aggatcattt tgcagaggat actacacttt attgaaatca
     5641 gcgacttaat tataccctgg cggatccatg ttggcgcaca gatcgccagt tcagctataa
     5701 agatctgatg ctattcagaa aagatcctgc tccgaacgga acaagtgtcg atatatcatg
     5761 tgattactta actaacaccc tccacaaatc ccggatcaaa aagggagcag ctcacgaaac
     5821 ggcatttgaa cagaaactta cggtttcaac tgctgaacaa agaatgaaac cgtaagttct
     5881 tgaatgtggg attacgttaa cggtcggaac ttgccttgct cgacccgctg gttttaaaac
     5941 cccgccctta agggcgggga tataaggcgc ggtgtataag ttacgctaca tcaggaccat
     6001 catcacgctc cggcatacct gatactctga atcccttcat gacttcagat tcacgagtca
     6061 gcgatctaca gtcccaaacc ctgcgatcgc catttttttc aatctctctt ccatccttca
     6121 tcctttcaat ccactgaact ttctctgttc ctttatcctc cagtcgcaaa cgctcacctt
     6181 tactataacc tgctacagca ctctccagac caactcccca tagtgttcgt gttttcccgt
     6241 tatccatctt cagtttcaca aaatagctat catcacgttc atcttctggt ttgcttatat
     6301 caggcttaaa tttgtatgct gcccggccat gatcaagtag cacaccttca tgtactttat
     6361 ctttattacc tctcgactct gcttcccgat ggatttcttt ttgtgccaca gtggcgctaa
     6421 cttctgatac aggcacagga atactgctgt tccacgcaac cctcgctgca atttcactgt
     6481 taatttctgc tttaactgca tccaatttat ctactggagt agccatttcc cgcatttttt
     6541 gctccatcga taacgccatt ccacgtagtt catcatctga caaaatgacg gctaacggtt
     6601 ccctgctttg atctccacta ccgagacgat ttcttatttc ctgatcagtc tgttctctga
     6661 cggattcttt atctgcctgc aatgcagtaa attctctttc cttttgttcc gcagtcatag
     6721 ccatctgccg taatactgga ttagcaagaa taaggtcaac agctgtctgc gttctggctg
     6781 gtatggggct ttgattttgc tgtattttct ccagcgactg ttcccttttc tgctcctgtc
     6841 ccaccgacat cttgtttaat gtccaagtct gccggaccgc ttccctgata gacaaattgc
     6901 cgagatgatc gcgtgtactg acctgaaccg gattagattc tttcaactca agagatatct
     6961 ctgttccccg gcgtttcccc ttcattacgt tttgcaaatc actatcccag tgtgtggttt
     7021 ctttaccgtc cctgcccctg accgtaacaa aataactttg ttcagcaccc ttcttgttat
     7081 caagaggcgc tttgccgtga tctattaatt taccctccag cttaactact gtcctgcttt
     7141 caggtgattc gtgtggctct gaggactcct tcacatttgt ttgattttta gtatctgctg
     7201 cctgttcatg ctctttctct tgttccttac tcgtactcgt tttagaaacg ccttctattg
     7261 tcatcccatc acgttcagcg gcaatttctg cccgtcgttc ttcaagttgc cgattcatcg
     7321 ctttgtcaga aaaatgaaga tctgggtgat ttctggcgac aatatcaata acctggttac
     7381 gaaattcttc attccccttg atagtcagtg tactgccaaa acgttctttg gccagttcaa
     7441 gagcaatccc cagcgccgcc ggagacatac catcttttct tatagcaatg gcatttcctg
     7501 tatcaacgaa taatgtcctg ttactgtttt tatcaagata atgcacattc ttactgagtt
     7561 tcgattttct ggtatataaa tccgctgcac taacagactt tgcatatgaa ttctctttat
     7621 cttcaccttc aattcggttt ttcagtgtat gaactattct tgaaaaacgt tttcctgccg
     7681 gtggtatctt ccgctcgtct ttccctgtaa tttcgttatt tgacaggcgt ggttttttct
     7741 taataagttt gcggatatcc tcacttgcca tagtattctc ctgctgacta ctattaatga
     7801 ttttctgacg cgcttttaca tgctcaaaag cgagtactga cagatactgt gagcgttctt
     7861 tcctagttaa tcgttcatcc tgccagataa aactccgggt tgactgatag agctgccgaa
     7921 tgtcccacaa tgcctgacgt tgattaggta atatcttatt ttgttcatat tgacgaacaa
     7981 aatgagcggc tacataagct gcgtttttcc ccatttgggg tatatcacca ttaaacctgt
     8041 tcacactgat atttgatttt cctggaggtg gtcgtaaaag aatttcacta cggcgaatta
     8101 catcctgtat tgttgcccct ccgggtttat tacgcgcata cacaggaata ctcacatccc
     8161 cggccagtaa tcctccggca tatcgatttc cgtactgagt tcttgttgac cgtctggagt
     8221 gaacggtggc attcgtcccg gtaatagcgg gttgtccgct tcctcgttga ttaccagatc
     8281 gtcctcttcc tcctgaatgt acatcgcatc gcagtccaga atctcttccg ctggattgcg
     8341 actgtcccat gaaaacaagt gcatcagtgg acagaaacaa cgaactcccg gtgtttccag
     8401 tttgccaacc agttgccatt tcgccaccgg acatactttg caagccattg ggagagaagg
     8461 tagttcctct ggtctgagct tcattaatac cggacttatg atttgttttc cattcggcag
     8521 ggaatacgtc atatctttct ccgtacttct gataaaatgc tgtttcttgt tgtgcaagaa
     8581 tttttatttt ctcctcctgc gaggatgaat aatatttttt tcggaaatca ctgttagcct
     8641 tatcaatgta cttaagctcc atagctcgct gtggccattc atgcaaaagc tgctgaatga
     8701 cagatttttc cagtggaggt ttcttcagtt ctcgtctcac aataaagtca tcattaaata
     8761 ttgtctcctt caggttggta aactttttat cacccggtaa tttaacggcg atatattcat
     8821 tttctttacc ctgattacga attcggatat caccaaatga agatacatgt gtataaaaat
     8881 cttttcttga taaaatattt tcttcaacaa tatccctgac cagttgttct ttaaattctc
     8941 tgttcttact tctgaaatca tcccccttat aacgagacaa aatatcagac acattctttg
     9001 gattggctct gacacgctct cggggagaaa tcagattata tttttggttt atgtattcct
     9061 gaacagcctc aaaatatttt tcatttaccc tgtaatctcc cctggggttt attaaattac
     9121 cagaaagaag attaacctct ggaataataa tatgaacatg tggttttctt tctatcaatt
     9181 caccggtctt cttatcttcc gagtat
//
LOCUS       DAZKGF010000187         9134 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZKGF010000187 DAZKGF010000000
VERSION     DAZKGF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.187"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(5..1996)
                     /gene="tkt"
                     /locus_tag="ACHOQV_004292"
     CDS             complement(5..1996)
                     /gene="tkt"
                     /locus_tag="ACHOQV_004292"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9632574.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            2274..3032
                     /gene="loiP"
                     /locus_tag="ACHOQV_004293"
                     /gene_synonym="yggG"
     CDS             2274..3032
                     /gene="loiP"
                     /locus_tag="ACHOQV_004293"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFP9632575.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3080..4000)
                     /gene="speB"
                     /locus_tag="ACHOQV_004294"
     CDS             complement(3080..4000)
                     /gene="speB"
                     /locus_tag="ACHOQV_004294"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFP9632576.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4136..4867)
                     /locus_tag="ACHOQV_004295"
     CDS             complement(4136..4867)
                     /locus_tag="ACHOQV_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632577.1"
                     /translation="MKKWKVRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERVWRDPAEMIVISYSTTVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYIRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(5013..6989)
                     /gene="speA"
                     /locus_tag="ACHOQV_004296"
     CDS             complement(5013..6989)
                     /gene="speA"
                     /locus_tag="ACHOQV_004296"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFP9632578.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(6998..7129)
                     /gene="yqgB"
                     /locus_tag="ACHOQV_004297"
     CDS             complement(6998..7129)
                     /gene="yqgB"
                     /locus_tag="ACHOQV_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFP9632579.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(7223..7366)
                     /gene="yqgG"
                     /locus_tag="ACHOQV_004298"
     CDS             complement(7223..7366)
                     /gene="yqgG"
                     /locus_tag="ACHOQV_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFP9632580.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(7477..7728)
                     /gene="yqgD"
                     /locus_tag="ACHOQV_004299"
     CDS             complement(7477..7728)
                     /gene="yqgD"
                     /locus_tag="ACHOQV_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFP9632581.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            7784..8938
                     /gene="metK"
                     /locus_tag="ACHOQV_004300"
     CDS             7784..8938
                     /gene="metK"
                     /locus_tag="ACHOQV_004300"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP9632582.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2235 a         2469 c         2339 g         2091 t
ORIGIN      
        1 ccgattacag cagcgctttc gctttcgcaa caacgttgtc aacggtgaaa ccgaactctt
       61 caaacagttg ctctgcagga gcagattcac cgaaggtggt catgccgacg atagcaccgt
      121 tcaggccaac atacttgtac cagtagtcag caatacccgc ttccaccgca acacgagcgg
      181 taaccgcttt cggcagtacg gattcacggt aagccgcatc ctgcttgtcg aacgcgtcgg
      241 tagacggcat ggaaaccacg cgcgccttca cgccttcggc agtcagtttt tcgtaggcgg
      301 caacagccag ttcaacttcc gaaccggtcg cgatgaagat cagttccggc tgaccggcac
      361 agtctttcag cacataacca ccgcgcgcga tgtttgccag ctgctcttca gtacgctcct
      421 gctgcgccag gttctgacgg gagaggatca gcgcggtcgg gccgtcctga cgctcaacgc
      481 cgtatttcca cgcgaccgcg gattcaacct ggtcacatgg acgccatgtg gacatgttcg
      541 gggtcacgcg cagagaagcg acctgctcta ccggctggtg agtcgggcca tcttcgccca
      601 gaccgatgga gtcgtgggtg taaaccatca cctgacgctg tttcatcagc gcagccatac
      661 gtacggcgtt acgtgcgtat tccacgaaca tcaggaaggt ggaggtgtac ggcaggaaac
      721 caccgtgcaa ggagataccg ttagcaatcg cggtcatacc gaactcgcga acaccgtagt
      781 ggatgtagtt acccgcagca tcttcgttga ttgctttaga accagaccac agggtcaggt
      841 tagacggtgc caggtcagca gagccgccga ggaattccgg caacagcggg ccgaaagctt
      901 cgatagcatt ctgagacgct ttacggctgg cgattttcgc cggattagcc tgcagtttag
      961 cgatgaattc tttcgctttg gcatcgaagt cagacggcat ttcgcctttc atacggcggg
     1021 taaattcagc ggcttcctgc ggataagctt tggcgtaagc cgcgaatttc tcgttccatg
     1081 cggattcttt cgcctggcct gcttctttcg catcccactg agcatagatt tcagacggga
     1141 tttcgaacgg tgcatatttc cagcccagct gttcgcgggt cagggcaatt tcagcgtcgc
     1201 ccagcggcgc accgtgggag tcgtgcgtac cggctttgtt cggggaaccg aaaccgatga
     1261 tggttttgca catcagcagg gacggtttgt cagtcactgc gcgcgcttct tctactgcgc
     1321 gtttgatgga tgccgcgtca tgaccgtcga tgtcgcgaat aacgtgccag ccgtaagctt
     1381 cgaaacgcat tgcggtgtcg tcggtaaacc agccttcaac gtgaccatcg atagaaatac
     1441 cgttgtcatc gtagaacgca atcagtttac ccagcttcag cgtacccgcc agagagcaaa
     1501 cttcgtggga gatgccttcc atcatgcagc cgtcgcccat gaaggcgtag gtgtagtggt
     1561 cgacgatgtc gtggcccgga cggttaaact gcgccgccag cgttttttct gcaatcgcca
     1621 taccgactgc gttggcaata ccctgaccca gcggacctgt agtggtttcc acgccagcgg
     1681 tgtaacccac ttccggatgg cccggagttt tagagtgcag ctgacggaag tttttcagtt
     1741 cttccatcgg cagatcgtaa ccggtgaggt gcagcaggct gtagatcagc atggagccgt
     1801 ggccgttgga cagcacgaag cggtcacggt cagcccagga cggattctgc gggttgtgtt
     1861 tcaggaaatc acgccacagg acttcggcaa tgtcagccat acccatcggg gcgcccgggt
     1921 gaccggattt ggctttctgt actgcgtcca tgctcagcgc acgaatagca ttggcaagct
     1981 ctttacgtga ggacattttg actccagatc ggatgatgaa gggcacgccc ttaacgactt
     2041 gacgacagcg cgttttgggc tacgccggaa aatttgccaa caatttaccg caagccgcgc
     2101 gtcatgtaca tggaacatcc ttttgccgct tcagaaatct ctggatcatg ctcgcatgtt
     2161 gcgcaatctg ctcgcccgtc cgctgcgctt ttccttatac tgagactgag cgtcgattca
     2221 cctgcaaacg gcgcattttt agaataatcc tgaccttgtg cggaagagaa aacatgaaaa
     2281 ttcgcgcctt attggtagca atgagcgtgg caacggtact gaccggttgc cagaatatgg
     2341 actccaacgg actgctctca tcaggagcgg aagcttttca ggcttacagt ttgagtgatg
     2401 cgcaggtgaa aaccctgagc gatcaggcat gtcaggagat ggacagcaag gcgacgattg
     2461 cgccagccaa tagcgaatac gctaaacgtc tgacaactat tgccaatgcg ctaggcaaca
     2521 atatcaacgg tcagccggtg aattacaaag tgtatatggc gaaggatgtg aacgcctttg
     2581 caatggctaa cggctgtatc cgcgtctata gcgggctgat ggatatgatg acggataacg
     2641 aagtcgaagc ggtgatcggt cacgaaatgg ggcacgtggc gttaggccat gtgaagaaag
     2701 ggatgcaggt ggcacttggt acaaatgccg tgcgagtagc tgcggcctct gcgggcggga
     2761 ttgtcggcag tttatctcaa tcacaactcg gtgatctggg cgagaaatta gttaattcgc
     2821 aattctccca gcgccaggaa gcagaagccg atgattattc ttacgatctt ctgcgccaac
     2881 gcggcatcag cccggcaggt cttgccacca gttttgaaaa actggcgaag ctggaagaag
     2941 gccgtcaaag ctcaatgttt gacgaccatc cagcatccgc cgaacgcgcc cagcatattc
     3001 gcgatcgtat gagcgcggat ggagttaagt aagcatttta ttacgccgga tttgacgcat
     3061 aacgcatctt atccggcgct tactcgccct ttttcgctgc ctgaatatac agcatctcca
     3121 gcgccagcgt cgccgcagcc agagcggtga tttccgactg atcgtatgcc ggagccactt
     3181 ccactacgtc catcccaaca atgttgagat ctttcaggcc gcgtaccagt ttaatagcgc
     3241 gatcggaggt caggccgcca attactggcg taccggttcc cggcgcaaat gcaggatcca
     3301 ggcagtcgat atcaaaggtc aggtaaaccg gcatatcacc cacaatctgt ttcacttggg
     3361 cgataacgtc atccacgctg cgatcgttca cctggcaggc gtccagcacg gtaaagccgt
     3421 tgtctttatc gaactcagta cgaataccaa tctgcacgga atgattcggg tcgatcagac
     3481 cttctttcgg cgcggtatag aacatggtgc cgtggtcaaa ttcacaaccg ttcgcatagg
     3541 tgtcggtgtg ggcgtcaaag tgtaccagcg ccattttgcc gaaatgcttc gcatgagcac
     3601 gcagcagcgg cagcgtaaca aagtggtcac caccgaagga gagcatacgc ttaccggcag
     3661 ccagcagctt ctcggcgtgc gcctgcagct tctcgctcat ctcacgggca tctccgaagg
     3721 catataccag atcgccgcag tccacgacgt tcaggcgctc acgcatgtcg aaattccacg
     3781 ggaagcgatt gtgttcccag gccagattcg tcgaaacctg acggatcgct gccggaccgt
     3841 ggcgaccacc cgcacgacca gaagtggcca tatcgaacgg cacgccagta atcacccagt
     3901 cagcatcgct gtcatatggc tggaagttca tcggcaggcg taaaaaacca aaggcgttgg
     3961 aaaccagtga gttatcgtat tgatgaccta aggtgctcat ggatggactc ctattacaaa
     4021 gtcgatataa aaaaaccctt ccgcgtcgtt aggcccgacg aggaagggtt ggatttgcga
     4081 aacacaattt tgtgctaatt atcgccttaa atgggcgagg catcaagcga ggttgctatt
     4141 tccgacacgg tggcggcgca tagccattgt tatctgccgg aaaggcttgt ccagagcacg
     4201 gcggcagcgc gttttgcgcc gggccttgta attcaaaggc ggcaaagccg attacaccgg
     4261 tattacgcac atcgccttgc gccgaattgg ctgcgtagga atcagcaacg ttggcgaact
     4321 gaaaagccgc ttcggtatgt ttatctttgc ggaatccttt aatcgctaac gaatctccgg
     4381 cattcacaat atagccgttg ttattcagcg aaccttgctt gccgttcagc acatccaggc
     4441 catcaaccgt agcaacgatc tcgtagttgg tattacgact gtaattacgg atatataatt
     4501 gataattcat tccgactctg gcatggacaa tatagcgtgg ttcattattg gatgaatcat
     4561 aaacccgcgt aataggtaga gaattaaaat taccatcgcg aatagcatat tccagttcgt
     4621 taatacgaat gctataaacg cggtcatagc cggaaggaac agtagtcgaa taactaataa
     4681 cgatcatctc tgccggatcg cgccagactc gttcagcaga tacgccctga accgatgagt
     4741 gtacatcccc accccaggcg gtgccgacat tgcctgatgc actgctatta tactgcgcat
     4801 tactggaaca accggccagc agcacggcta atgctatcaa cgcgctacgt actttccatt
     4861 ttttcataac cgttccctct gcatttaatt aatgcagcga ttgtaggggg attatttaat
     4921 aagaaaagca gctaaattat ttttaagaat atataaaaag aaagcgggcc gatttacgta
     4981 aataaatcga cccgccagcg gaataatctt aattattcat cttcaagata ggtataaccg
     5041 tacaaacctg cctcgaactc ttcaaggaac tgttgttgca gttcagcatc aagatcggtt
     5101 ttcttcactt gatcacggaa ctgggttaac aacgttttcg gatcgagctg tacatattgc
     5161 agcatatccg ccacggtatc gccttcgtca gacagttcta cttctacgct gccgtcaggg
     5221 aagacgaaca cgtcaaccgc ttcggtatca ccgaacaggt tgtgcatgtt gccgaggatc
     5281 tcctgatatg cgccgaccat gaagaaaccg agcatcggcg gattctctgg atcgtactcc
     5341 ggcattggca ttgtcgtggc aataccatca ccatcgatgt agtgatcaat agcaccgtca
     5401 gaatcacagg taatatccag cagcacagcg cggcgctccg gcacttgatc caacccttcc
     5461 agcggcagaa ccgggaacaa ctggtcgatc ccccatgcgt ccggcatcga ctggaacagc
     5521 gagaagttga cgtacatttt gtcagccata cgttcctgca gctcgtcgat aatcggacga
     5581 tgcgcacggt tttgcggatc cagttgcttc tgcacttcat ggcacatgct cagatagagt
     5641 tgttctgccc atgcacgttc ttgcaggcta aaggtgccgg aagagtagcc gatatgaata
     5701 tcgtgcagat ccatctggct gtcgtgtaac cattcacgca gagaacgacg agttcccggt
     5761 tcgtgcatct cctgccaggt ttcccacatg ctttgcaggg cgcgcggtgc gtcttctgca
     5821 ggcgcggtcg gcaccgtgta ttcgttacgt tccacgccga tgatattcga caccagcacg
     5881 gtgtgatgcg cagtcaccgc acgacccgac tcggtgatta ccgtcggatg tggcagacca
     5941 ttttcttcac acgcatcacc aatcgcccag atgatgttgt tggcgtattc attgaggccg
     6001 tagttcaccg aacagtcgga ctgcgaacgg gtgccttcat agtccacgcc cagaccgccg
     6061 ccaacgtcga agcactgaat attgacgccc agcttgtgca gttccacata gaaacgcgcg
     6121 gattcacgaa cgcctgtcgc gatatcgcga atattcgcca tctgcgaacc gaggtggaag
     6181 tgcagcagtt gcaggctgtc gagacgcccg gcttcacgca gcgtttcaac cagttgcagt
     6241 acctgagtcg ccgccaggcc gaacttcgat ttttccccgc cagaggactg ccatttaccc
     6301 gagccctgcg aagccagacg tgcacgcacg cccagacgag gaacgacatt cagacgttct
     6361 gcttcatcca gcacaatggc aatttctgac atcttctcaa tgaccagata gaccttgtgg
     6421 cccatcttct cgccaattaa tgccaggcgg atatattcgc ggtccttata accgttgcag
     6481 acgatgacgc tacgggtcat gccagcatgt gccagcaccg ccatcaactc cgctttggaa
     6541 ccggcttcca ggcccagcgg ttcgcccgaa tgaatcaggg actcaatcac gcggcggtgc
     6601 tggttaactt tgatcggata aacgaggaag taatcgccgt tatagccgta ggattcccgc
     6661 gcacgtttga acgcggcgtt aatagaacgc aaacggtgct gcaggatctg tgggaaacag
     6721 aacagtgcag gcagacgctg gccctgtgct tcacgagttt tcactaactg cgcgagatcg
     6781 acgcgagctt ccgggacgtc cgggtccggg cacacgctaa tgtggcccag ctcgttaacg
     6841 tcatagtagt tattgcccca ccaggcaata ttgtaagtac gcagcatctt gctggcttcc
     6901 tgggagctca ttgcaacctc ctgcatggag cgtagtacac cgtgttcgcc cgctgacgaa
     6961 ggcaaaccca tagacatgtc gtcagacata gcgaacctca aattatttta ttaagtgtaa
     7021 aacagttaac gactatcgca gcctggaact gcgataacaa cccataagca catggatttt
     7081 ccagcagtga atgctgacgc tccaactgcg caaccggttt ctttttcata acattattaa
     7141 gcacataacc gaacgtaagt gtgacagttc ggcgaaacca cgagaaaact cttgttttta
     7201 caagagcgcc cttgttcagt cctcagtaac tgtaaccagc tcttgaatcc tgagaagcgc
     7261 cgagatgggt ataacatcgg caggtatgca aagcagagat gcagagtgcg gggaacgaat
     7321 cttcaccaga acggtgagac aggttaagca gcagacaacg gttcattatt tcgtatcacc
     7381 tccacggccg cctgttaaga cgaacccaca agccaaaact ctgatttcaa cccggctgga
     7441 agtggcaaca cgaaagaaac gtcgtgtgct ttttatttaa gccgcgcgcc gcgttttata
     7501 ccccacaatg gcagaaaatt gcaaaagata aatacgcaga atgccagcat tgtcaggaaa
     7561 aatttccagc cacgttttta acagaatgag acacgattca aaaaaaagtg gaaatcgggt
     7621 gaagaattga cctaaaatag ccatccagat gttaatccat ccataccgat taacactcag
     7681 actgccagtg tttttaacct gcagagtcgt ggtaggatcc gctaccacag aaaatccaca
     7741 caacagtttg agctaaccaa attctcttta ggtgatatta aatatggcaa aacacctttt
     7801 tacgtccgag tccgtctctg aagggcatcc tgacaaaatt gctgaccaaa tttctgatgc
     7861 cgttttagac gcgatcctcg aacaggatcc gaaagcacgc gttgcttgcg aaacctacgt
     7921 aaaaaccggc atggttttag ttggcggcga aatcaccacc agcgcctggg tagacatcga
     7981 agagatcacc cgtaacaccg ttcgcgaaat tggctatgtg cattccgaca tgggctttga
     8041 cgctaactcc tgtgcggttc tgagcgctat cggcaaacag tctcctgaca tcaaccaggg
     8101 cgttgaccgt gccgatccgc tggaacaggg cgcgggtgac cagggtctga tgtttggcta
     8161 cgcaaccaat gaaaccgacg tgctgatgcc agcgcctatc acctatgcac accgtctggt
     8221 acagcgtcag gctgaagtgc gtaaaaacgg cactctgccg tggctgcgcc cggacgcgaa
     8281 aagccaggtg actttccagt atgacgacgg caaaatcgtt ggtatcgatg ctgtcgtgct
     8341 ttccactcag cactctgaag aaatcgacca gaaatcgctg caagaagcgg taatggaaga
     8401 gatcatcaag ccgattctgc cggctgaatg gctgacttct gccaccaaat tcttcatcaa
     8461 cccgaccggt cgttttgtta tcggtggccc gatgggtgac tgcggtctga ctggtcgtaa
     8521 aattatcgtt gatacctacg gcggcatggc acgtcacggt ggcggtgcat tctccggtaa
     8581 agatccatca aaagtggacc gttccgcagc gtacgcagca cgttatgtcg cgaaaaacat
     8641 cgtggctgct ggcctggccg atcgttgtga aattcaggtt tcctacgcaa tcggcgtggc
     8701 tgaaccgact tccatcatgg tagaaacttt cggtactgag aaagtgcctt ctgaacaact
     8761 gactctgctg gtacgtgaat tcttcgacct gcgcccatac ggtctgattc agatgctgga
     8821 tctgctgcac ccgatctaca aagaaaccgc agcatacggt cactttggtc gtgaacattt
     8881 cccgtgggaa aaaaccgaca aagcgcagct gctgcgtgat gctgccggtc tgaagtaatc
     8941 tttcttcacc tgctttcaaa ggccagcctc gcgctggcct ttttctttta cctcaccgcg
     9001 gtggacaggc attcacgccg catccggcaa aaaaaccgcc cgcacaataa catcattcct
     9061 cctcaacacg ttttaccgca aattaccatc actactgaaa ccgattacac caaccacgcc
     9121 agccaaatat ttgt
//
LOCUS       DAZKGF010000188         8809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZKGF010000188 DAZKGF010000000
VERSION     DAZKGF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.188"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            53..148
                     /gene="ymiC"
                     /locus_tag="ACHOQV_004301"
     CDS             53..148
                     /gene="ymiC"
                     /locus_tag="ACHOQV_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFP9632583.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            298..2973
                     /gene="acnA"
                     /locus_tag="ACHOQV_004302"
     CDS             298..2973
                     /gene="acnA"
                     /locus_tag="ACHOQV_004302"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFP9632584.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGNITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDDVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3037..3627)
                     /gene="ribA"
                     /locus_tag="ACHOQV_004303"
     CDS             complement(3037..3627)
                     /gene="ribA"
                     /locus_tag="ACHOQV_004303"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFP9632585.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            3797..4561
                     /gene="pgpB"
                     /locus_tag="ACHOQV_004304"
     CDS             3797..4561
                     /gene="pgpB"
                     /locus_tag="ACHOQV_004304"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFP9632586.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            4710..5018
                     /gene="lapA"
                     /locus_tag="ACHOQV_004305"
     CDS             4710..5018
                     /gene="lapA"
                     /locus_tag="ACHOQV_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFP9632587.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            5025..6194
                     /gene="lapB"
                     /locus_tag="ACHOQV_004306"
     CDS             5025..6194
                     /gene="lapB"
                     /locus_tag="ACHOQV_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFP9632588.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMALLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            6385..7122
                     /gene="pyrF"
                     /locus_tag="ACHOQV_004307"
     CDS             6385..7122
                     /gene="pyrF"
                     /locus_tag="ACHOQV_004307"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP9632589.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAARFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7122..7448
                     /gene="yciH"
                     /locus_tag="ACHOQV_004308"
     CDS             7122..7448
                     /gene="yciH"
                     /locus_tag="ACHOQV_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFP9632590.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(7574..7792)
                     /gene="osmB"
                     /locus_tag="ACHOQV_004309"
     CDS             complement(7574..7792)
                     /gene="osmB"
                     /locus_tag="ACHOQV_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFP9632591.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8061..>8809)
                     /gene="yciT"
                     /locus_tag="ACHOQV_004310"
     CDS             complement(8061..>8809)
                     /gene="yciT"
                     /locus_tag="ACHOQV_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFP9632592.1"
                     /translation="NSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKLS
                     YLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLAR
                     TLGEQKKNVTIITVSSYIAHLLKDAPCEIILLGGVYQKKSESMVGPLTRQCIQQVHFS
                     KAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVER
                     FNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         2154 a         2088 c         2341 g         2226 t
ORIGIN      
        1 taacaggttg agttatctca acacaaagta ataaccgtta aaggtgtagc ctatgatcaa
       61 cacaaatatg aaatattggt cctggatggg cgcattttct ctgtcgatgc tcttctgggc
      121 cgaactcctc tggatcatta ctcactgatc cctgaccccg ctgtttgctt tgtctcaagg
      181 tttctcctct tttatcaatt tgggttgtta tcaaatcgtt acgcgatgtt tgtgttatct
      241 ttaatattca ccctgaagag aatcagggct tcgcaaccct gtcattaagg aggagctatg
      301 tcgtcaaccc tacgagaagc cagtaaggac acgttgcagg ccaaagataa aacttaccac
      361 tactacagcc tgccgcttgc tgccaaatca ctgggcaata tcacccgtct gcctaagtca
      421 ctcaaagttt tgctcgaaaa cctgctgcgc tggcaggatg gtaactcggt taccgaagag
      481 gatatccacg cgctggcggg atggctgaaa aatgcccatg ctgaccgtga aattgcctac
      541 cgcccggcaa gggtgctgat gcaggacttt accggcgtac ctgccgttgt tgatctggcg
      601 gcaatgcgcg aagcggttaa acgcctcggt ggcgatactg caaaggttaa cccgctctca
      661 ccggtcgacc tggtcattga ccactcggtg accgtcgatc gttttggtga tgatgaggca
      721 tttgaagaaa acgtacgcct ggaaatggag cgcaaccacg aacgttatgt gttcctgaaa
      781 tggggaaagc aggcgttcag tcgttttagc gtcgtgccgc caggcacagg catttgccat
      841 caggttaacc tcgaatatct cggcaaagca gtgtggagcg aattgcagga cggtgaatgg
      901 attgcttatc cggatacact cgttggtact gactcgcaca ccaccatgat caacggcctt
      961 ggcgtgctgg ggtggggcgt tggtggcatc gaagccgaag ccgcaatgtt aggccagccg
     1021 gtttccatgc ttatcccgga cgtagtgggc tttaaactca ccggaaaatt acgtgaaggt
     1081 attaccgcca cagacctggt tctcaccgtt acccaaatgc tgcgcaaaca tggcgtggtg
     1141 gggaaattcg tcgaatttta tggtgatggg ctggattcac taccgctggc ggatcgcgcc
     1201 accattgcca atatgtcgcc agaatatggt gccacctgtg gcttcttccc aatcgatgct
     1261 gtcaccctcg attacatgcg tttaagcggg cgcagcgaag atcaggtcga gttggtcgaa
     1321 aaatatgcca aagcgcaggg catgtggcgt aacccgggcg atgaaccaat ttttaccagt
     1381 acgttagaac tggatatgaa tgacgttgaa gcgagcctgg cagggcctaa acgcccacag
     1441 gatcgcgttg cactgcccga tgtaccaaaa gcatttgccg ccagtaacga actggaagtg
     1501 aatgccacgc ataaagatcg ccagccggtc gattatgtta tgaacggaca tcagtatcag
     1561 ttacctgatg gcgcggtggt cattgctgcg ataacctcgt gcaccaacac ctctaaccca
     1621 agtgtgctga tggccgcagg cttgctggcg aaaaaagctg taactctggg cctcaagcgg
     1681 caaccgtggg tcaaagcgtc gctggcacca ggctcgaaag ttgtttctga ctatctggca
     1741 aaagcgaaat tgacaccgta tctcgacgaa ctggggttta accttgtggg gtacggttgt
     1801 accacctgta ttggtaactc tgggccgctg cccgatccta tcgaaacggc aatcaaaaaa
     1861 ggcgatttaa ccgtcggtgc ggtgctgtcc ggcaaccgta actttgaagg ccgtatccat
     1921 ccgctggtta aaactaactg gctggcctcg ccgccgctgg tggttgccta tgcgctggcg
     1981 ggaaatatga atattaacct cgcttctgag cctattggtc acgatcgtaa aggcgacccg
     2041 gtatatctga aagatatctg gccctcggca caagaaattg cccgtgcggt agaacaagtc
     2101 tccacagaaa tgttccgcaa agagtacgca gaagtttttg aaggcacagc agagtggaag
     2161 gaaattaacg tcacacgatc cgatacctac ggttggcagg aagattcaac ctatattcgc
     2221 ttatcgccat tctttgatga aatgcaggca acaccagcac ctgtggaaga tattcacggt
     2281 gcgcggatcc tcgcaatgct gggggattca gtcaccactg accatatctc tccggcgggc
     2341 agtattaagc ccgacagccc ggcgggtcga tatctacaag gtcggggtgt tgagcgaaaa
     2401 gactttaact cctacggttc gcggcgtggt aaccatgaag tgatgatgcg cggcaccttc
     2461 gccaatattc gcatccgtaa tgaaatggtg cctggcgttg aaggggggat gacgcggcat
     2521 ttacctgaca gcgatgatgt ctctatctat gatgccgcga tgcgctataa gcaggagcaa
     2581 acgccgctgg cggtgattgc cgggaaagag tatggatcag gctccagccg tgactgggcg
     2641 gcaaaaggtc cgcgtctgct tggtattcgt gtggtgattg ccgaatcgtt tgaacgaatt
     2701 caccgttcga atttaattgg catgggcatc ctgccgctgg aatttccgca aggcgtaacg
     2761 cgtaaaacgt tagggctaac cggggaagag aagattgata ttggcgatct gcaaaaccta
     2821 caacccggcg cgacggttcc ggtgacgctt acgcgcgcgg atggtagcca ggaagtcgta
     2881 ccctgccgtt gtcgtatcga caccgcgacg gagttgacct actaccagaa cgacggcatt
     2941 ttgcattatg tcattcgtaa tatgttgaag taacaagtat ttgcttgccg gttattttgc
     3001 ttccggcaag caaatgaatt acacaatgca agagggttat ttgttcagca aatggcccat
     3061 tttctcggct ttggtatcga gataatgttc gttattgggg ttacgaccta caatcaatgg
     3121 tacgcgttca acaatattaa tccctgcttc ggtcagaatt tcgacttttt tcgggttatt
     3181 ggttaacaag cggacttcat tgacgccaag gagtttgaac atatcagcgc aaagagtgaa
     3241 gtcgcgctca tcagcggcga agcctaactg gtggttagcc tctacagtat cgtaaccttg
     3301 atcctgcagt gcataagcgc ggattttatt cagcagacca atgttacgac cttcctgacg
     3361 gtgatacagc aaaataccac ggccttcttc ggcaatttgc gtcagtgccg cttcgagctg
     3421 gaagccacaa tcgcagcgca agctgaacag ggcgtcaccg gtcagacatt cggaatggac
     3481 gcgcgcaagt accggggtat gcccggagat atcgccatag actagcgcga catgatcgtg
     3541 tccggttgcc agttcttcaa atcccaccat caggaaatcg ccccatgggg ttggcagttt
     3601 ggcttctgcc acacgtttaa gctgcatgaa attctccaga taatgctggt tctgtattgg
     3661 cttattttgc cataacgaga aggggttcac ctaatcacga cgcgtcgatc gttcacggaa
     3721 tggcacaaat ctgtcaattt ttcctggaac tggcgttttc agttatgatt gtgggactta
     3781 tcaaaaagga gaggccatgc gttcgattgc cagacgtacc gcagtgggag ctgcactatt
     3841 gcttgtcatg ccagtagccg tatggatttc tggttggcgt tggcaacctg gagaacaaag
     3901 ttggctacta aaagcggctt tttgggttac tgaaactgtc acccagccct ggggcgtcat
     3961 tacacatttg attttattcg gctggtttct ctggtgtctg cgttttcgca ttaaggctgc
     4021 ctttgtatta tttgccattc tggcggcggc aattcttgtg ggacaaggcg ttaaatcctg
     4081 gatcaaagac aaagtccagg aaccacgacc ttttgttatc tggctggaaa aaacacatca
     4141 tattccggtt gatgagttct acactttaaa gcgagcagaa cgcggaaatc tggtgaaaga
     4201 acagttggct gaagagaaaa atatcccaca atatttgcgt tcacactggc agaaagagac
     4261 ggggtttgcc tttccttccg gtcacacgat gtttgctgcc agttgggcgc tgctggccgt
     4321 tggtttgctg tggccgcgtc ggcgaacgtt aaccattgct atcttgttgg tctgggcaac
     4381 gggagtcatg ggaagccgcc tgctgctcgg gatgcattgg ccacgcgatc tggtggtagc
     4441 tacgttgatt tcgtgggcgc tggtggcggt ggcaacgtgg cttgcgcaac gaatttgtgg
     4501 gccattaaca ccacctgcgg aagaaaatcg cgaaatagcg caacgagaac aagaaagtta
     4561 acgctggttg attttccgaa tttagccctt aaatcatcat caatgcgcgt ggatgccatt
     4621 tcgcagacgg cgcgaaaatg gtactttaaa gggctattgc ggtaagttga ccataattta
     4681 ttcgctctaa ccacataacg ggaagtaatg tgaaatattt actcattttc ttactggtgt
     4741 tagcgatctt cgtgatttcg gtcacgttag gtgcgcagaa cgatcaacag gtgacgttta
     4801 attatctgtt agcgcaaggg gagtaccgta tttccacatt gctggcggta ttgtttgctg
     4861 cggggtttgc tatcggttgg ctgatttgcg gtctgttctg gctgcgagtt cgtgtttccc
     4921 tggcgcgcgc tgaacgtaaa ataaagcgac tggaaaacca gctttcaccc gcgactgacg
     4981 tggctgtagc gccgcattcg tcagcggcga aggaataact ttctatgctg gagttgttgt
     5041 ttctgctttt gcctgtagcc gctgcctatg gctggtatat gggccgcaga agtgcgcaac
     5101 aaaacaagca agatgaagcc aaccgcttgt cgcgtgatta cgtagcgggg gttaacttcc
     5161 tgcttagtaa tcaacaggat aaagcggtag acctgtttct cgatatgctt aaagaggata
     5221 cgggtaccgt tgaagcccac cttacgctag gaaacctgtt ccgttcgcgt ggcgaagttg
     5281 atcgcgctat tcgcatccat cagaccctaa tggaaagcgc ctcgctgacc tatgaacagc
     5341 gtctgttggc gattcaacaa ctggggcgtg attacatggc cgccgggtta tacgaccgcg
     5401 cggaagacat gttcaatcag ctgaccgatg aaactgactt ccgcattggc gcgctgcaac
     5461 agttgctaca aatctaccag gctaccagcg agtggcagaa agcaattgat gttgccgaac
     5521 gtctggtgaa gttgggtaaa gataagcagc gcgtcgaaat tgcccatttc tactgtgagt
     5581 tagccctgca gcatatggcc agcgacgatc tcgatcgcgc catggccttg ttaaaaaaag
     5641 gtgcggcggc agataaaaac agcgcccgcg tatccatcat gatgggacgc gtatttatgg
     5701 cgaaaggaga atacgccaaa gccgtcgaaa gtctgcaacg cgtgatatct caggacagag
     5761 aactggtcag cgaaacgctg gaaatgttgc aaacctgcta tcagcagttg ggtaaaactg
     5821 ccgaatgggc agaattcctg cagcgcgcgg tggaagagaa caccggtgcc gatgctgaat
     5881 tgatgcttgc ggatattatc gaagcgcgcg acggtagtga ggccgcacag gtctatatta
     5941 cgcgtcagct tcagcgtcat ccgaccatgc gtgtgttcca taagctaatg gattaccact
     6001 taaatgaagc ggaagaaggg cgtgccaaag agagtctgat ggtgctgcgt gacatggttg
     6061 gcgagaaggt gcgtagtaag cctcgttatc gctgccagaa atgtggtttt accgcataca
     6121 ccctctactg gcattgtccg tcttgtcggg cctggtcaac cattaaaccg attcgcggtc
     6181 ttgatggcct gtaattttta aaaaaatccg actttagtta caacatacta ataattgatg
     6241 ttcattgtgc tccggtaact ccggcgcaga aaagcctgcc aggggagaaa tcgcaactgt
     6301 taatttttta tttccacggg tagaatgctc gccgtttacc tgtttcgcgc cacttccggt
     6361 gcccatcatc aagaaggtct ggtcatgacg ttaactgctt catcttcttc ccgcgctgtt
     6421 acgaattctc ctgtggttgt tgcccttgat tatcataatc gtgatgccgc gctggccttt
     6481 gtcgacaaga tcgacccacg cgattgtcgt ctgaaagtcg gcaaagagat gtttacgctg
     6541 tttggacctc aatttgtacg cgaacttcaa cagcgtggtt ttgatatctt cctcgacctg
     6601 aaatttcacg atattcccaa cactgcagcg cacgctgtcg ctgctgcagc tgacttaggc
     6661 gtgtggatgg tgaatgttca cgcctccggt ggggcgcgta tgatgaccgc agcgcgtgag
     6721 gcactggtgc cgtttggcaa agatgcaccg cttttgattg ctgtgacagt attgaccagc
     6781 atggaagcca gcgacctggc cgatcttggc gtgacactgt cacctgcaga ttatgcagaa
     6841 cgtctggcgg cactgacgca aaaatgtggc cttgatggtg tggtgtgttc tgctcaggaa
     6901 gctgcgcgct ttaaacaggt atttggtcag gagttcaaac tggttacgcc gggcattcgt
     6961 ccgcagggga gtgaagctgg tgaccagcgc cgcattatga cgccagaaca ggcgttggcg
     7021 gctggtgttg attatatggt gattggtcgc ccggtaacgc aatcggtaga tccagcgcaa
     7081 acgctgaaag cgatcaacgc ctctttacag cggagtgcat gatgagtgat tccaacagcc
     7141 gtctggtcta ctcaacggaa accggacgta ttgatgagcc aaaagcggcc cctgtacgcc
     7201 ctaaaggcga cggtgtggtg cgtattcagc gtcagaccag tggacgtaaa ggtaagggcg
     7261 tttgcctgat taccggtgtc gatctcgatg atgccgaact gacaaaactg gcagcggaac
     7321 tgaagaaaaa atgcggctgc ggcggggcag ttaaagatgg aattattgaa atccagggcg
     7381 ataagcgtga tttattaaaa tcactgcttg aagcgaaagg gatgaaggta aaactcgccg
     7441 gtggttaaca taaaaagcca cggatatatc cgtggctttc gaatatttta ctgtgtgtat
     7501 tattcagtta tttcgtactg aacagaatca agcgtattat aattgcgagt cgataatcga
     7561 catttattta cgattattta ccgacctggt gaccaataac accaccgaca gctgcgccac
     7621 ctaatgtacc caacgtactg ccatcggtca gtactgcacc gcctaatgcc cctgcacccg
     7681 cgccgattgc ggtgttgcga tcccgtttag accagttaga acaggcactc agagacattg
     7741 ccagggtaat tgccagaaca gccgcggtca tttttttgct cgttacaaac ataatactct
     7801 ctcctgaatt tatgattcac ggaagtaagc tctctataac tataatagct aagaataagc
     7861 ctggtgaaat tattccgtga aatccgctcg cgcgcagggt tatatcacgc aggtgataat
     7921 gacttcctgt tatatcgctg ataataattt tatatcttga gagtgttaat aacaggtaaa
     7981 tagtcttaat tatcaaccag gaatcatctt agagcggatg atttgccaaa gtgcaaatca
     8041 tccgtagaga agggaaatgg ttaaatgtca acaacgtgaa cggtgagttt actgtgctca
     8101 agatgcatca gatcgctggc gcgtattttc gaatcggtaa tcacgcgatt gaatcgctca
     8161 acgggaccga tggagtatga atgtacagca ccaaatttcg agctgtcagt caggactatc
     8221 gcttcgcact ctttttccag cacggcattg accacatcgg tacgcatcat atcgcgaccg
     8281 gtgaatccag tttcaggttg ccagccatca ataccaataa atgctttgct gaaatgtacc
     8341 tgttggatac actggcgtgt caacgggcca accatacttt cgcttttttt ctggtacacg
     8401 ccaccgagca aaataatttc acaaggcgcg tctttcagca aatgcgcgat gtagctgctg
     8461 accgtgatga tagtgacatt tttcttctgc tcgcccagag tccgggcgag cagggcattg
     8521 ctgctgccat tttcgataaa gatggtttcg cccggttgaa ccagtgacgc ggcaaactcg
     8581 gcgagttcac gcttcagcgt atagttgctc atcatacggg tttcgacgtc atcactatca
     8641 agcgatactg caaagccatg tgcacggcgg aggtaactca gtttttcgag ggtgttgaga
     8701 tcctggcgaa tggtaacttc agaaactcca gtggcttttg ccagatcggt tacgctaacc
     8761 tgaccctggt caatgaccat ctgtagaata gtttgttgtc gggaattca
//
LOCUS       DAZKGF010000189         8473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZKGF010000189 DAZKGF010000000
VERSION     DAZKGF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.189"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            94..984
                     /gene="alsR"
                     /locus_tag="ACHOQV_004311"
     CDS             94..984
                     /gene="alsR"
                     /locus_tag="ACHOQV_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFP9632593.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            1043..1978
                     /gene="alsB"
                     /locus_tag="ACHOQV_004312"
     CDS             1043..1978
                     /gene="alsB"
                     /locus_tag="ACHOQV_004312"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFP9632594.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            2106..3638
                     /gene="alsA"
                     /locus_tag="ACHOQV_004313"
     CDS             2106..3638
                     /gene="alsA"
                     /locus_tag="ACHOQV_004313"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFP9632595.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNIAISRSLKDGGYKGAMGLFHEVDEQRTAEAQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCHPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     HE"
     gene            3617..4597
                     /gene="alsC"
                     /locus_tag="ACHOQV_004314"
     CDS             3617..4597
                     /gene="alsC"
                     /locus_tag="ACHOQV_004314"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFP9632596.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            4608..5303
                     /gene="alsE"
                     /locus_tag="ACHOQV_004315"
     CDS             4608..5303
                     /gene="alsE"
                     /locus_tag="ACHOQV_004315"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFP9632597.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDNHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            5287..6216
                     /gene="alsK"
                     /locus_tag="ACHOQV_004316"
     CDS             5287..6216
                     /gene="alsK"
                     /locus_tag="ACHOQV_004316"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFP9632598.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            6489..>8473
                     /gene="yjcS"
                     /locus_tag="ACHOQV_004317"
     CDS             6489..>8473
                     /gene="yjcS"
                     /locus_tag="ACHOQV_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl/aryl-sulfatase"
                     /protein_id="HFP9632599.1"
                     /translation="MNNSRLFRLSRIVIALTAASSMMVNTANATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETI
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAKEANKQGDYRWSAELLKQVIAANPGDQVAKNLQADNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPEANASFYISREDLHAVLTGQVKMV
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         2203 a         2046 c         2161 g         2063 t
ORIGIN      
        1 ataaatttta tttttttgtg aagttgccag catcttttct gttcttgctg tggtgatata
       61 gtggcgtctt caattcaagg acaagagaac gtgatgagcc agtcagagtt tgattcagcg
      121 cttccgaacg gtatcgggtt agcgccttac ctgcgaatga agcaggaagg aatgacagaa
      181 aacgaaagcc gcatcgtgga gtggttactc aaacccggta acctgagttg tgcacccgca
      241 attaaagatg tcgcagaagc tctggcggta tctgaagcga tgatcgttaa ggtatcaaag
      301 ctgctggggt ttagcggctt tcgtaactta cgcagtgcgc tggaagatta tttttctcag
      361 tcagaacaag tattgccttc cgagttggct tttgatgaag cgccgcagga tgtggtgaat
      421 aaggtattta acatcacttt acgcaccatt atggaaggtc agtcgatcgt caacgttgat
      481 gagatccacc gtgccgcccg ctttttctat caggccagac agcgggattt gtacggtgcc
      541 ggaggatcaa atgcaatctg tgctgatgta cagcacaagt ttttgcgcat tggcgtacgc
      601 tgccaggcct atccggacgc tcacatcatg atgatgtctg cttcgttgtt acaggaagga
      661 gatgtggtgc tggtggtgac ccattccggg cgaaccagtg atgtaaaagc ggctgtagaa
      721 ctggcaaaaa agaacggggc aaagattatt tgtataacgc atagctacca ttcaccgata
      781 gcgaagctgg ccgattatat tatttgctca ccggccccgg aaacaccgtt attaggtcgt
      841 aatgcctcgg caagaatatt acaactaact ttgctggacg cgttttttgt ctctgtcgcc
      901 cagctcaaca ttgaacaagc taatattaat atgcaaaaaa ccggcgcaat tgttgatttc
      961 ttctcaccag gcgcgctgaa ataagaaaaa ggtaattccg ccatcgcgga gttacctatc
     1021 cccttaccgt gagaacatga ttatgaataa atatctgaaa tatttcagcg gcacactcgt
     1081 gggcttaatg ttgtcaacca gcgcttttgc tgccgccgaa tatgccgtcg tattgaaaac
     1141 actctccaat ccattttggg tagatatgaa aaaaggcatt gaagatgaag caaaaacgct
     1201 gggcgtcagc gttgatattt ttgcctctcc ttcagaaggc gattttcaat ctcaattgca
     1261 gttatttgaa gatctcagta ataaaaatta caaaggtatc gccttcgcac cattatcctc
     1321 agtgaacctg gtcatgcctg tcgcccgcgc atggaaaaaa ggcatttatc tggtcaatct
     1381 cgatgaaaaa atcgacatgg ataatctgaa aaaagcaggc ggtaatgtgg aaggttttgt
     1441 caccaccgat aatgttgccg tcggggcgaa aggcgcgtca ttcattattg acaagctggg
     1501 tgccgaaggg ggtgaagtcg caatcattga gggtaaagcc ggtaacgcct ccggtgaagc
     1561 gcgtcgtaat ggtgccaccg aagccttcaa aaaagcaagc cagatcaagc ttgtcgccag
     1621 ccagcctgcc gactgggacc gtattaaagc actggatgtc gccactaacg tgttgcaacg
     1681 taatccgaat attaaagcga tctattgcgc gaatgacacg atggcaatgg gtgttgctca
     1741 ggctgtcgca aacgccggaa aaacgggaaa agtgctggtc gtcggtacag atggcattcc
     1801 ggaagcccgc aaaatggtgg aagccggaca aatgaccgcg acggttgccc agaacccggc
     1861 ggatatcggc gcaacgggtc tgaagctgat ggttgacgct gaaaaatccg gcaaggttat
     1921 cccgctggat aaagcaccgg aatttaaact ggtcgattca atcctggtca ctcaataaaa
     1981 ctaaatgatg ccggatggtg atgctaatga agtgtcttat ccggccaaca aaataacctg
     2041 aacgtagacc tgataagtgt agcccatcag gcaataatac gatatttcgt gcagagaggt
     2101 tatttatggc cacgccatat atatcgatgg cggggatcgg caagtccttt ggtccggttc
     2161 acgcattaaa gtcggttaat ttaacggttt atcctggtga aatacatgca ttactaggag
     2221 aaaatggcgc gggtaaatcc acgctaatga aagttttatc cggaatacat gagccgacca
     2281 aaggcaccat taccattaat aacattaact ataacaaact ggatcataaa ttagcggcac
     2341 aactcggcat cgggattatt tatcaagaac tcagcgttat tgatgaatta accgtactgg
     2401 aaaatttata tattggtcgt catctgacga aaaaaatctg tggcgtcaat attatcgact
     2461 ggcgagaaat gcgtgtccgc gccgccatga tgttattacg cgtgggcttg aaagttgatt
     2521 tagatgagaa agtggcgaat ttatctatca gccacaagca gatgctggaa attgccaaaa
     2581 cgctgatgct cgacgccaaa gtcatcatca tggatgaacc cacctcctca ctcactaata
     2641 aagaggtgga ctatttgttt ctgatcatga atcagttgcg taaggagggt acggccatcg
     2701 tctatatctc gcataaattg gcggaaattc gccgtatttg cgaccgctat acggtgatga
     2761 aagacggcag cagcgtttgc agcggcatgg tgagcgatgt gtcaaatgac gatatcgtcc
     2821 gtctgatggt aggtcgcgaa ctgcaaaacc gttttaacgc gatgaaggag aatgtcagca
     2881 accttgcgca cgatacggtt tttgaggtgc gcaacgtcac cagccgtgac agaaaaaagg
     2941 tccgggatat ctcatttagc gtctgccggg gagaaatatt aggctttgcc ggactggtcg
     3001 gttccggacg tactgaactg atgaactgcc tgtttggcgt tgataaacgc gctggcggag
     3061 aaatccgtct taatggcaaa gatatctctc cacgttctcc cctggatgcc gtgaaaaaag
     3121 ggatggctta catcactgaa agccgccggg ataacggctt tttccccaac ttttctatcg
     3181 ctcagaacat agctatcagc cgcagtctga aagacggcgg ctataaaggc gcgatggggt
     3241 tgtttcatga agttgacgag caacgtaccg ctgaagccca gcgcgaactg ctggcgctga
     3301 aatgtcattc ggtaaaccag aacatcactg aactctccgg cggtaaccag cagaaagtcc
     3361 tgatctccaa atggctgtgc tgtcacccgg aagtgatcat tttcgatgaa cctacccgtg
     3421 gcatcgacgt tggcgcgaaa gccgaaattt acaaagtgat gcgccaactg gcggacgacg
     3481 gaaaagtcat cctgatggtg tcatctgaac tacctgaaat tatcaccgtc tgcgaccgca
     3541 tcgccgtgtt ctgcgaagga cgactgacgc aaatcctgac caatcgcgat gacatgagcg
     3601 aagaggagat tatggcatgg gctttaccac acgagtaaaa agcgaagcga gcgagaagaa
     3661 accgttcaac tttgcgctgt tctgggataa atacggtact ttttttatcc tggcgatcat
     3721 cgtcgccatt tttggttcgc tgtcgccaga atattttctg accaccaata atattaccca
     3781 gatttttgtg caaagctccg tgacggtact gatcggcatg ggggaattct tcgctatcct
     3841 ggtcgccggt atcgaccttt cggttggcgc gattctggcg ctttccggta tggtgaccgc
     3901 taaactgatg ttggcaggta ttgatccgtt tctcgcggcg ctaattggcg gtgtactggt
     3961 tggcggcgca ctgggggcga tcaacggctg cctggtcaac tggacgggac tacacccgtt
     4021 cattatcacc cttggcacca acgctatttt ccgtgggatc acgctggtga tctccgatgc
     4081 caactcggta tacggcttct catttgactt cgtgaacttc tttgccgcca gcgtaattgg
     4141 gatacctgtc cccgttatct tctcgctaat tgtcgcgctc atcctttggt ttctgacaac
     4201 gcgtatgcgg ctcgggcgca acatctacgc actgggcggc aacaaaaact cggcgttcta
     4261 ttccgggatt gacgtgaaat tccacatcct ggtggtgttt atcatctccg gtgtttgtgc
     4321 aggtctggca ggcgtcgtct caactgcacg actcggtgcc gcagaaccgc ttgccggtat
     4381 gggttttgaa acctatgcca ttgccagcgc catcattggc ggcaccagtt tcttcggcgg
     4441 caaggggcgc attttctctg tggtgattgg cgggttgatc atcggcacca tcaacaacgg
     4501 tctgaatatt ttgcaggtac aaacctatta ccaactggtg gtgatgggcg gattaattat
     4561 cgcggctgtc gcccttgacc gtcttatcag taagtaagga attgaacatg aaaatctccc
     4621 cctcgttaat gtgtatggat ctgctgaaat ttaaagaaca gatcgaattt atcgacaacc
     4681 atgccgatta ctttcatatc gatatcatgg acggccactt tgtgccgaat ctgacgctct
     4741 caccgttctt cgtaagccag gtaaaaaaac tggcgagtaa accgctcgac tgccatctga
     4801 tggtaacgcg tccgcaggat tacattgctc aactggcgcg tgcgggagct gatttcatca
     4861 ctctgcatcc ggaaaccatc aacggccaag cgttccgcct gattgatgaa atccgccgtc
     4921 atggcatgaa agtggggctg attcttaatc cggaaacgcc ggttgaggcc atgaaatact
     4981 atatccataa ggctgataaa attacggtca tgactgtcga tcccggtttt gccggacaac
     5041 cgttcattcc tgaaatgctg gataaacttg ccgaactgaa ggcatggcgt gaacgagaag
     5101 gtctggagta cgaaattgag gtggacggtt cctgcaacca ggcaacttac gaaaaactaa
     5161 tggcggcagg ggcggatgtc tttatcgtcg gcacttccgg cctatttaat catgcggaaa
     5221 atatcgacga agcatggaga attatgaccg cgcagattct ggctgcaaaa agcgaggtac
     5281 agcctcatgc aaaaacagca taacgtcgta gcgggcgtgg atatgggggc aacgcatatc
     5341 cgcttttgtc tgcggacagc agaaggtgaa acgctacact gcgaaaaaaa gcggaccgca
     5401 gaagttattg ctcccgacct ggtgtcgggt atcggcgaaa tgattgacga gcaactcagg
     5461 cgctttaacg ctcgctgtca tggtctggtg atgggatttc cggcgctggt cagtaaagat
     5521 aaacgcacca ttatttctac gcctaacctg ccgttaacag cggcagattt atatgatctc
     5581 gccgataagc tcgaaaatac gctgaattgt ccggttgagt tttcccgcga cgttaacctg
     5641 caactctcct gggacgtagt agaaaatcgc cttacgcaac aactggttct ggcagcctat
     5701 ctcggtacgg ggatggggtt cgcagtgtgg atgaacggtg cgccgtggac gggtgcgcac
     5761 ggtgtggcag gcgaactggg ccatatcccc ctgggagata tgacccaaca ctgcgcgtgt
     5821 ggcaattctg ggtgcctgga aaccaattgc tctggaatgg cgctaagacg ctggtacgaa
     5881 caacagcccc gaaattaccc attgagcgat cttttcgtcc atgcggaaaa cgcccctttc
     5941 gtccagagtc tgcttgaaaa cgcggcacga gccattgcca ccagcattaa tctgttcgat
     6001 cccgatgcgg tgattctggg tggtggcgtg atggatatgc ccgccttccc acgcgagact
     6061 ctcattgcca tgacccaaaa gtacctgcgc cgtccactgc cgtatcaggt tgtgcgcttt
     6121 attgccgcct catcttctga ctttaatggc gctcagggtg cagcaatatt ggcgcatcaa
     6181 cgttttttgc cacagtcctg tgctaaagtc ccatgagtaa agcgttggca cagtagcgca
     6241 atgtcccgct gtgccaacga caggtacaat atatataata attacataaa acagcccata
     6301 ttattcccag caatgatagt tctactgtac ttttccctct tcagcacaaa tgcctaataa
     6361 aatggcgcgt tcagctcaca cttctactta ttaaagtcgc aatttcccaa ttatgataac
     6421 gccagcttta aatgacgtat tttgcatgga ttacatattt acgacataaa tgataaatgg
     6481 agatttatat gaataactct cggttattcc gtttgagcag gattgttatt gcgttaactg
     6541 ccgccagcag catgatggta aataccgcta atgcaacaga tgaagcgaaa gccgccactc
     6601 aatataccca acaggttaat cagaattacg ccaaatcatt accgtttagc gatcgtcagg
     6661 attttgacga tgctcagcgt ggatttatcg ctccgctgct ggatgaaggt attctgcgtg
     6721 atgcgaacgg taaaatttac taccgcgcga atgattacaa atttgatatt aatgccgctg
     6781 cgccggaaac cataaacccc agcctgtggc gtcagtcgca gatcaacggc atttcaggcc
     6841 tgttcaaagt caccgataaa atgtatcagg tgcgcggcca ggatatctct aacattacgt
     6901 tcgttgaggg cgagaaaggc attattgtta tcgacccgct ggtgacgccg cctgcagcaa
     6961 aagccgcact tgatctttac ttccagcatc gtccgcaaaa accgattgtt gccgttatct
     7021 acactcacag ccacgccgac cactatggcg gcgtgaaagg tattatctct gaagccgatg
     7081 ttaaatccgg caaagtgcag gtgattgctc cggcaggctt tatggacgaa gccatcagcg
     7141 aaaacgtgct ggcgggtaac atcatgagcc gccgtgcgct ctactcttac ggtctgttac
     7201 tgccgcacaa cgcgcaaggc aacgtgggca acggcctggg cgtgacgctg gcaacgggcg
     7261 acccgagcat tattgcaccg actaaaacta tcgtcagaac tggcgagaag atgattatcg
     7321 acggcctgga atttgacttc ctgatgactc cgggtagcga agcgccagcc gaaatgcact
     7381 tctatattcc ggccctgaaa gccctgtgta ccgccgagaa cgccacgcat accctgcaca
     7441 acttctacac tctgcgcggc gcgaaaaccc gcgataccag caagtggacc gagtacctga
     7501 acgaaacgct ggatatgtgg ggtaacgacg cggaagtact gtttatgccg cacacctggc
     7561 cggtctgggg caataagcat atcaatgatt atattggtaa atatcgcgat accatcaagt
     7621 acattcacga ccagaccctg cacctggcga accagggcta caccatgaat gaaatcggcg
     7681 acatgattaa gctgccgcct gcgcttgcca ataactgggc cagccgcggc tattacggtt
     7741 ctgtcagcca caacgcccgc gcggtgtata acttctatct tggctattac gacggtaacc
     7801 cggctaacct gcatccgtat ggtcaggtgg agatgggtaa acgttacgtg caagctctgg
     7861 gcggttctgc ccgcgtcatc aacctggcga aagaagcgaa caagcaaggt gattaccgct
     7921 ggtcggcaga actgctgaaa caggtgattg ccgccaaccc tggtgaccag gtcgcgaaga
     7981 atctgcaagc ggataacttt gaacagctgg gctatcaggc cgagtccgcc acatggcgcg
     8041 gtttctacct gaccggcgcg aaagagctgc gcgaaggggt acataagttc agccacggca
     8101 ccaccggttc cccggacacc attcgcggaa tgtcggtcga aatgctgttc gactttatgt
     8161 ccgttcgcct cgatagtgcg aaagccgcgg gtaaaaatat cagcctgaac ttcaatatga
     8221 gcaacggcga taacctcaac ctgacgctga acgatagcgt gcttaactac cgtaaaacgc
     8281 tgcaacctga agccaacgcc tctttctaca tcagccgtga agatctgcat gccgtgctga
     8341 ccggacaagt caaaatggtg gatctggtaa aagcgaagaa agccaaaatt attggcaatg
     8401 gcgcgaaact ggaagaaatt attgcctgtc tggataattt cgatttgtgg gtgaatatcg
     8461 taaccccaaa tta
//
LOCUS       DAZKGF010000190         8426 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZKGF010000190 DAZKGF010000000
VERSION     DAZKGF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.190"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            199..702
                     /gene="ftnB"
                     /locus_tag="ACHOQV_004318"
     CDS             199..702
                     /gene="ftnB"
                     /locus_tag="ACHOQV_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFP9632600.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(780..1031)
                     /gene="yecJ"
                     /locus_tag="ACHOQV_004319"
     CDS             complement(780..1031)
                     /gene="yecJ"
                     /locus_tag="ACHOQV_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFP9632601.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1146..1232)
                     /gene="azuC"
                     /locus_tag="ACHOQV_004320"
     CDS             complement(1146..1232)
                     /gene="azuC"
                     /locus_tag="ACHOQV_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFP9632602.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1495..1818
                     /gene="yecR"
                     /locus_tag="ACHOQV_004321"
     CDS             1495..1818
                     /gene="yecR"
                     /locus_tag="ACHOQV_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFP9632603.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1990..2487
                     /gene="ftnA"
                     /locus_tag="ACHOQV_004322"
     CDS             1990..2487
                     /gene="ftnA"
                     /locus_tag="ACHOQV_004322"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFP9632604.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2525..2764)
                     /gene="yecH"
                     /locus_tag="ACHOQV_004323"
     CDS             complement(2525..2764)
                     /gene="yecH"
                     /locus_tag="ACHOQV_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFP9632605.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2954..4165
                     /gene="tyrP"
                     /locus_tag="ACHOQV_004324"
     CDS             2954..4165
                     /gene="tyrP"
                     /locus_tag="ACHOQV_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFP9632606.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4216..4881)
                     /gene="yecA"
                     /locus_tag="ACHOQV_004325"
     CDS             complement(4216..4881)
                     /gene="yecA"
                     /locus_tag="ACHOQV_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFP9632607.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIESAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALDAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5238..6239)
                     /locus_tag="ACHOQV_004326"
     CDS             complement(5238..6239)
                     /locus_tag="ACHOQV_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9632608.1"
                     /translation="MSVRKLSTGKWLCECYPYGAAGKRIRKQFATKGEALSYERRLMN
                     SNLGGEFQDGSGPRLSELVARWFEMYGKTLSSGEERKAKLEAICSRLGDPFASQFDKN
                     MFAIYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYADEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQILP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDTAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(6274..6531)
                     /locus_tag="ACHOQV_004327"
     CDS             complement(6274..6531)
                     /locus_tag="ACHOQV_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632609.1"
                     /translation="MVTNPQIPLTGLYVSKVNPSNRIVVTDVHIVLDDDDEPGDLPFY
                     LVTFVNEGDEDDMSAPSWELDPDEWEQLVDEKLFMRVEQSS"
     gene            complement(6729..7370)
                     /locus_tag="ACHOQV_004328"
     CDS             complement(6729..7370)
                     /locus_tag="ACHOQV_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632610.1"
                     /translation="MVVNSNGSDNRVAGRDYHEKNIQIERYDGRHTVNIAIPSNRTDE
                     RPLVKAQRRQLNLLIDNIVDVSKEESFVVWRKLHAEIGVDSIDNMTVNQYPTAVSFLN
                     AMLDRYKDHDACKSLVSLLLRNSENNEVRQKLLRYCHINFGTGRLNDLTRSQLQMALS
                     WLDQQSVEGEKKLTANNSAGSQLQMNFQQIIKKYPKETGMCFVIGVLLGIILF"
     gene            complement(7386..7778)
                     /locus_tag="ACHOQV_004329"
     CDS             complement(7386..7778)
                     /locus_tag="ACHOQV_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011147635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9632611.1"
                     /translation="MNFSSLCGARLKAERKRLSLKQADAAALCGVSRESWGKYERGTM
                     VPGGDVLLSFALNGANVQYILTGEAGGVVLNRDEMEIIKHFRNAPLAVKAAALAALTA
                     GSSASNTVNVSGRGHRIAGRDYNETKGR"
     gene            complement(7869..8006)
                     /locus_tag="ACHOQV_004330"
     CDS             complement(7869..8006)
                     /locus_tag="ACHOQV_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632612.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSADNRSASRGPVAVLRGIRDLV
                     S"
     gene            8078..8281
                     /locus_tag="ACHOQV_004331"
     CDS             8078..8281
                     /locus_tag="ACHOQV_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9632613.1"
                     /translation="MQGITININVNAPYVSLKKYAEITGIPLNTCKKMLADGRIIIRP
                     KRARMEKPEVNLIAMLKDAIANS"
     gene            8302..>8426
                     /locus_tag="ACHOQV_004332"
     CDS             8302..>8426
                     /locus_tag="ACHOQV_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP9632614.1"
                     /translation="MKKNAIPPRPKHRTGTERHANRFSTSASRSNSHYSLQETHAT"
BASE COUNT         2249 a         2072 c         1768 g         2337 t
ORIGIN      
        1 aaatgtaaaa atatataaac ttgatgattt aagcattttc ttatacccgt tcagacgtta
       61 ttcttatttc agatcatcgt cagaattgac tccacgatca catttcggac cggcagaaag
      121 gaattattct gcaaacagta attatggtgt tttgatttat cttgcacctc tccacttctg
      181 gatataagga tattaggtat ggcaaccgct ggaatgcttc tcaaactcaa ctctcaaatg
      241 aaccgcgagt tttacgcatc caatctctac cttcacctga gtaactggtg ttctgaacag
      301 agtctgaacg gcaccgccac tttccttcgc gcccaggcac agagtaatgt gacccaaatg
      361 atgcgcatgt ttaactttat gaagagtgtc ggcgctaccc ccatcgttaa agccattgat
      421 gttcccggtg aaaaactgaa ctctctggaa gaactgttcc aaaaaacgat ggaagaatac
      481 gagcaacgtt ctagtacgtt ggcacagtta gccgatgaag cgaaagaact gaatgatgat
      541 tcaaccgtca atttcctgcg cgatctggaa aaagaacagc agcatgatgg cctgttgctg
      601 caaaccattc ttgatgaagt gcgcagtgcg aaacttgcgg gtatgtgccc tgtgcagacc
      661 gaccaacatg ttctgaatgt cgtgtcgcat cagctacatt gatcatcatc ggcgctaatg
      721 cattgcgccg atgaagtttt tgagaaaccg ctgcctcatt tttttgaagc agcggttttt
      781 taatgggatt cgccctgtgg ggtaaacttg agttcaatca acgcgatggc tttttggatt
      841 gcccgcatgg tgaccgggtc tgcggcggcg ggatggttag taaagtcgat attctttagc
      901 tggctggaca ttttttcgcg aacttcaacg ggtgcgatca catcgagaac atccagaatt
      961 tgtttgataa ccaactggca agcaaccaca tcagaaacca gttcctgatc ggcattcagc
     1021 ggctgggaca tcgtaaactc ctgatagcat tttgaaagcc gttatagtag cggcttcaca
     1081 tcttcagcga tagtcacatc caccgtcatc aggacacaaa aaaacctgcc ggagcaggtt
     1141 ttttgttatc ggaacatatt gcctggcggt acgtctttga acgtcttgca atagttattg
     1201 aacatacttt tcaggatttt gcgcagtttc atcgcggcac tccgaccatt tgttatacag
     1261 gtgttattgt ctttgcgctt ataatgtgac aaccatcaca aaaatcaatc tttatgtgat
     1321 gcaaatcaca taaatacccc tttaatgtta taaaaatgat aatcaaaaag cagctacccc
     1381 atttctgaca cctacagatg gcaagaaata gcgcctgcca ggcgtctttt ccggccattg
     1441 tcgcagcgct gtagcgcgta aaatagtgct ttcttttact cttctggctg gaccatgaga
     1501 cttctgattc tgactctttc attaataacg ctggcggggt gtacggttac tcgccaggcc
     1561 catgtgagcg aagttgacgc ggcaacaggt attgttcggt tggtttatga tcaggctttt
     1621 ttgcagcatg cccgtaccga tcgttatgtc agtcgcggga tcgccgatcg cgcatgccag
     1681 caggcaggct atacccacgc tatccccttt ggtcagccag taggcaactg tagcctcttt
     1741 gcgggttcct tatgtttgaa tacggaattc actttgtcat atcagtgcca tcattccgcc
     1801 ttccctgtct ttctctaaac gttttaatca gcgaggggga tcttcgctga ttaaataaat
     1861 agcggtaatg cgttttattc ccattcgtat ttttaataaa tgatgtttat attttacctt
     1921 ttgcaaataa taaaataaca aattatagtg acgccacagc aacaaatata acctttgtgg
     1981 agcactatca tgctgaaacc agaaatgatt gaaaaactta atgagcagat gaacctggaa
     2041 ctgtactctt cactgcttta tcagcaaatg agcgcctggt gcagctatca taccttcgaa
     2101 ggtgctgccg cgttcctgcg ccgtcacgcc caggaagaga tgacgcatat gcagcgtctg
     2161 tttgattacc tgactgatac cggcaactta ccgcgtatta ataccgttga atctccgttt
     2221 gctgaatatt cctcacttga tgaattattc caggaaacct ataaacacga acaattaatc
     2281 acgcagaaaa ttaacgaact ggctcatgct gcaatgacca atcaggacta cccaacattt
     2341 aatttcctgc aatggtatgt ttctgagcag catgaagaag agaaactgtt caaatcgatt
     2401 attgataaat taagcctggc aggcaaaagt ggcgaaggtc tgtattttat cgacaaagaa
     2461 ctctctaccc tcgacacaca aaactaatgc tctccggcgg caggttttct gccgccgttt
     2521 caaatcagtg acggcaaatc ttactctgat cggttgaaaa accttcttct gaaggtataa
     2581 atttgccttt tgctgccaga aacgccacca actctcccgc agtcatccct tctgccgagc
     2641 aggtgtgaaa acgtgcctgt tcaccaaaac gcgctttaat tgctgcttcc agactggcat
     2701 gcgtatattg ctcgcctgat tcaatcatca tatttaacac ttcgtgaccg tgaatagagt
     2761 ccatcgttcc tcctcaaaaa aagcctagcg tagcgattgc cgcttatgaa gactttgcgc
     2821 cagcgcagaa ctgaacgctt ttattgtaca tttatattta catcatatgt aacgccggtt
     2881 tgacgaatca gccgttatgc cttaacctgc gccgcagata tcactcataa agatcgtcag
     2941 gacagaagaa agcgtgaaaa acagaactct gggaagtgtt tttatcgtgg cgggaaccac
     3001 aattggcgca ggcatgctgg caatgccgct ggctgcggca ggtgttggtt ttagcgtcac
     3061 gttaatcttg ttaattgggc tttgggcgtt gatgtgctac acggcgctat tactgctgga
     3121 ggtgtaccag catgtcccgg cagataccgg tctgggcacg ctggcaaaac gctatctggg
     3181 acgctacggt cagtggctga cgggcttcag tatgatgttc ttaatgtatg ctctgactgc
     3241 ggcatacatc agcggtgccg gtgaattgtt ggcctccagc atcagcgact ggacaggtat
     3301 ttcaatgtcg gcaaccgctg gcgtgctgtt gttcactttt gttgccggtg gcgtggtttg
     3361 tgtcggaact tcgctggtcg atttatttaa ccgttttctg ttcagcgcca aaattatttt
     3421 tctggtggtg atgctggtat tgctgctacc gcatattcac aaagtgaatc ttttaaccct
     3481 gccgttgcaa cagggactgg cactgtctgc aatcccggtg atttttacgt cgtttggttt
     3541 tcacggtagc gtgccgagta ttgtcagcta tatggatggc aacattcgta agctacgctg
     3601 ggtgtttata atcggtagtg cgatccccct ggtggcatat attttctggc agctggcgac
     3661 ccttggcagc attgattcaa caacctttat gggattgctg gctaatcatg ccggattaaa
     3721 cgggctgtta caggcgttac gcgaaatggt ggcctctccg catgttgagc tggcagtgca
     3781 tttatttgct gatttagccc tcgccacgtc cttcctcggc gtagcgttag gcttatttga
     3841 ttatctggca gatttgtttc agcgttcaaa taccgttggt ggacgattgc aaactggtgc
     3901 aattaccttt ctgccgccac tggcgtttgc actgttttat ccacgaggat ttgtgatggc
     3961 gctgggttac gccggtgtgg cgctggcggt actggcattg attatccctt cgctgttgac
     4021 ctggcaaagc aggaagcaca atcctcaggc gggttaccgg gtgaaaggtg gtcgtccggc
     4081 gctggtggtg gtgtttctct gtggtattgc tgtgattggc gtgcaatttt tgattgcggc
     4141 aggcttgtta ccagaagtgg ggtgattaga ttcttattgg gtgccagaat taacgctgac
     4201 acccaatttg acctcttaat gcaggcagca ctgcttaaat ttcttaccac taccgcacgg
     4261 acaaggatcg ttacgccccg gtttctcttc tgctttgata ggttgctgaa cagctttttc
     4321 ctgcggatgc gccatccagt atgcatgtaa atcaagcgcc gccagtcgaa tagcatctac
     4381 gctctcttca aacgcttctg gcgacatctt ctctacccgc tcgaagtttt cctcagtacc
     4441 gtgcagcgca atcgcatcca gcgctggttt taacgaatcg ggcaacgttg accagtcaga
     4501 aagtgccacg ccccgcatat agccaaagca ccactcctca acaatcgtca gctcgctgcc
     4561 atcgacttct cgcaagccga ataacggctc aaactgctcc gggaattcgt tcagacgctc
     4621 tgcggtatca gccatatgtt gaaaagccag gttcataaag cgcgtcatct ctttctctga
     4681 cgcccagcgc ggcacatagt cagccccacc ccacacggca accagccact gtgccgattc
     4741 aatctcttgc ggagaactca acaccgccgt caataaaccg tccagctccg ccacatcaag
     4801 aatggcgtgg tcagtgttgt atttggtcag aatatcgtcc agccattcca actcactttc
     4861 gtttaacggt cccgttttca tacgcttttc cttgtggatc tcaactcgcc agcacctatc
     4921 ttacatgccg gtccgtatca gagatacttt ttgagtggct ttgccggtga ttaaaaatta
     4981 aggagggtgt aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca
     5041 ctgcttattg ctttgatttt attcttatct ttcccatggt acccggagcg ggacttgaac
     5101 ccgcacagcg cgaacgccga gggattttaa aaactatcaa ccaccattta taaatcataa
     5161 cgttatgatt ttattaagta taaagataaa gcaatatggc gctatttgat gcttttgtta
     5221 cttttcatcg ccacttttta ccctctattg tcaaagggat tcagttcaac agcagcctct
     5281 aaatgacccg gcgcaaagtg tgcataacgc atagtcattt ttatatcgct atgccccagt
     5341 attttttgca acacaaggat atttcccccc cgcatcataa aatggctggc aaacgtatga
     5401 cgtagcacat gagttaattg cccatcagga agttcgatct ttgctctctt aatagcggtg
     5461 tcaaaagcct cataacatgg tgaaaaaagc gccccgcgtt ttttaggaag catggcctgc
     5521 aattgaggtg aaatcggtac agtgcggttc ttctttcctt tggttttaac aaatgtgatt
     5581 cgtccgggca gtatttgaga ttgctttaat ccctctgctt cactccacct ggcaccagtc
     5641 gcaagcccaa tacggacgac aacacccaaa tctttattcc gtgactcatc acatgcaatc
     5701 agaaggcgtt caatctcatc tgcatacaga aacgccagtt ccttttcttc ctcacgaaac
     5761 ttgcgaatac ctgtcaatgg attttcacca gaccactccc caaggcgctt cagttcagca
     5821 aaaacagcat gtaaatatga ctgttcgcga ttaacggttg cttcactaag ttttttcttc
     5881 ccctttggat tccattcacc tgataatcgc cgttccctgt agatggcaaa catattttta
     5941 tcaaattgag aagcaaacgg atcacccaac ctagaacaaa tcgcctcaag tttagccttt
     6001 cgctcttcac cggaagacaa cgttttaccg tacatttcaa accagcgagc aaccaactcg
     6061 gaaagacggg ggccagaacc atcctgaaac tcgcccccta aattactgtt cattaaacgg
     6121 cgctcataag agagcgcctc accttttgta gcaaactgct tacgaatgcg ttttcctgct
     6181 gctccgtagg gatagcattc acagagccat ttaccagtag atagtttacg aacagacata
     6241 ttaaaaagtg cacaccaagg aaaattttcg ttattaagat gattgctcaa cccgcataaa
     6301 gagcttttca tcaactaact gctcccattc atcaggatca agctcccaag aaggagccga
     6361 catatcatcc tcatccccct catttacaaa agtaaccaaa tagaaaggaa gatctccggg
     6421 ctcatcgtca tcgtccagga caatatgaac atcagtaacg actatacgat tagatggatt
     6481 aactttagat acatataaac cagttaatgg aatttgcgga ttagttacca taattcattt
     6541 acctctttgg acttcctcat gaataaagac ttacttttct ttaagaccat gacaagcaac
     6601 tcacaccatt tcttacatca ctagttgccc ctctcacctt tgtcagctcg cttaatttat
     6661 cttcttcatt tcttttaaag tcggtaaagc aaccttttgc cataatatct ttctccaaca
     6721 actatctatc agaaaagaat aatgcctaat aaaacaccaa taacgaaaca cattcctgtt
     6781 tcttttgggt attttttaat tatttgctga aaattcatct gtaattgact accggcagaa
     6841 ttgttggcag ttaatttctt ttctccttcc acagattgtt ggtctaacca ggaaagagcc
     6901 atctgaagct gagaacgagt aagatcgttt aaacgccctg taccgaaatt gatatggcaa
     6961 taccgcagaa gtttttgtct aacttcatta ttttcactgt tgcgaagaag gagacttaca
     7021 agagatttgc aggcgtcatg atccttatat cggtcaagca tcgcattgag aaaactaacc
     7081 gcggttggat actgattcac cgtcatgtta tcgatactat caacaccgat ttcagcatga
     7141 agtttacgcc aaacaacaaa cgattcttcc ttgctaacat caacaatgtt atcaatcaac
     7201 aagttgagtt gacggcgctg ggctttaact aatggacgct catcagtcct gtttgaaggg
     7261 attgcaatat taactgtatg acgaccatca taccgctcta tctgaatatt cttttcgtgg
     7321 taatcacgcc cagcaacgcg attatctgaa ccgtttgagt tgacgaccat ctcacctccc
     7381 tactatcacc tacccttagt ttcgttatag tcacgacctg caatacgatg gcctctacct
     7441 gatacgttta ccgtatttga tgcagaactc cccgcagtca gggcagcaag agctgcagcc
     7501 tttacggcaa gcggagcatt tctaaaatgc ttgataattt ccatctcatc acggtttagg
     7561 acaaccccgc cagcctcacc agttagtatg tattggacgt tagccccatt aagcgcaaat
     7621 gagagcaaaa catctccccc aggtaccata gttccccgtt cgtactttcc ccaagattct
     7681 cgcgatacgc cgcaaagagc agcagcatca gcctgtttga gagataaacg ctttctctca
     7741 gccttcagcc tcgcaccaca aagagatgaa aaattcactt ttacaccttg actaaagaga
     7801 tctattgatc tcataataac taaacaaacc attagatgat cacaatatac cactatgaaa
     7861 caagttcttc acgataccag atcacggatt ccgcgtaaca cagctacagg accgcgactg
     7921 gcacttcgat tgtcagcaga agagcgagcc gtcattgatg aaatggcagc taaagaacag
     7981 cgctcatcct ctaacatggc acgcatgatc ttccttcgcg gccttgagat tatccagaaa
     8041 gagcaaaacc aatcttcatg ctcaggaggt taacgggatg caaggtataa caataaacat
     8101 caacgttaat gccccttacg tatctctgaa gaaatacgcc gagataacag gcattcctct
     8161 taacacatgc aaaaagatgt tggctgacgg tcgaattatt atccgaccca agcgcgcaag
     8221 aatggaaaag ccagaagtaa accttatagc gatgttaaaa gacgcaatag ccaacagcta
     8281 aacaatgagg cgagcagaac tatgaagaaa aatgctattc ctcccagacc taaacaccgc
     8341 actggcacag aacgccacgc taaccgtttc tcaaccagcg catcacgcag caactcgcac
     8401 tacagcctgc aggaaacgca cgccac
//
LOCUS       DAZKGF010000191         8397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZKGF010000191 DAZKGF010000000
VERSION     DAZKGF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.191"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            31..1155
                     /locus_tag="ACHOQV_004333"
     CDS             31..1155
                     /locus_tag="ACHOQV_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFP9632615.1"
                     /translation="MTIHYTATLNNKHLSLTALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     SPGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            1136..3439
                     /gene="trbC"
                     /locus_tag="ACHOQV_004334"
     CDS             1136..3439
                     /gene="trbC"
                     /locus_tag="ACHOQV_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFP9632616.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEVIQSGDKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            3906..4136
                     /locus_tag="ACHOQV_004335"
     CDS             3906..4136
                     /locus_tag="ACHOQV_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632617.1"
                     /translation="MKTNMGSTKNGARVPHVRNRFTSMDIGMNSQILPDMLINFALIN
                     ITDRKNEGTNTIDGNWQADEGRRYRDNVRIYF"
     gene            complement(4234..4665)
                     /locus_tag="ACHOQV_004336"
     CDS             complement(4234..4665)
                     /locus_tag="ACHOQV_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFP9632618.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(4733..7444)
                     /locus_tag="ACHOQV_004337"
     CDS             complement(4733..7444)
                     /locus_tag="ACHOQV_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9632619.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDMQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPVSGHINRLSWSQERLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYEP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(7456..7788)
                     /gene="mobA"
                     /locus_tag="ACHOQV_004338"
     CDS             complement(7456..7788)
                     /gene="mobA"
                     /locus_tag="ACHOQV_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFP9632620.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            8021..8356
                     /locus_tag="ACHOQV_004339"
     CDS             8021..8356
                     /locus_tag="ACHOQV_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFP9632621.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKRAPKKTDSGR
                     NDGIDMNNN"
BASE COUNT         2137 a         2336 c         1922 g         2002 t
ORIGIN      
        1 acagcatccc gccgacaagg aaaaagaaca atgaccatcc actacactgc aacactgaat
       61 aataaacacc tgtccctgac cgccctgtac cctgacggta atcgggaggt gattgccatc
      121 ctgccagtca cagaactggg cgcactgatt aaccgtaata acaggctgat gcacctgtcc
      181 ttcagttccc cttttcgcga aataaccgaa acggtgcttg ttgataccgt tgatgcagaa
      241 cgtcccggac atcacatcgg cagaatgctc tcagcgattg atgcctgcct ggatgccgaa
      301 tatgccggaa aaggaaaaca tccctcccgg aaaaatggct tctggcggac actgatgtgt
      361 ggctttatcg ccggaattgc tctgccggcg acagttgcag gttaccactg gatgaaggca
      421 gagccccctt ccacgctggc ttaccctaca tctcaggaat ggagagctga accagtaacc
      481 gacacagaga caccacgccc acatacggaa catcatgtta acagcatccc ggcccgcccg
      541 gctcccacac agcaggaacc ttcttctgcc cccgccccga cggcagtgac ggaagaacaa
      601 cacgttcagg ccgctgaggc ccgccgtaat ctggccacag tacttaaacg caacgcagac
      661 cgggggatgt tcaccattaa cctgtcccct ggtcatgaac gcacgctgta cgccttcctt
      721 gaccccgcat gcatcaactg ccgtctgctg gaacccgcgc ttaagcaact ggcccgggaa
      781 ttcagcgtgg tgatttatcc ggtctccgtg attggcgggg agtccagtac aaagcggact
      841 gctccgctgc tgtgtgaacc ggactcacaa aaacgggcga ccggatggca tcagctgtat
      901 tccgctgaca gtggtatggc ccccaccggc gatgcaccgg ccgcacagga tggacagtgt
      961 ctgaccgccg cacgtgctgc catcgatgtc aataaccagg ctttccgcca gttcggtttt
     1021 ggcggaaccc cctgggtgtt atccgatacc ggctggcacc tgccgtccgg catcctgaaa
     1081 gacgccactt ccgttcgcct cttcctgaaa gcaacagata gcgaggagta tcacaatgag
     1141 ccagcaccac gttaatcctg acctcataca ccgcacggcc tggggaaacc cgttgtggaa
     1201 tgcactgcat aacctgaaca tcactggtct gtgtctggcc ggcagtatca ttacggcgct
     1261 gatatggcca ctggcccttc ctgtctgcct gctgttcacc ctggtcacct ccgtgatatt
     1321 catgctgcaa cgctggcgat gccccctgcg tatgcccatg acactcagcc ttgatgaccc
     1381 ctcccaggac agaaaggtca ggagaagcct cttcagcttc tggcccaccc tgtttcagta
     1441 tgaagcggat gaaacattcc ccgcccgggg gattttttat gtcggttacc gccgtatcaa
     1501 cgatatcggt cgtgagctct ggctgagcat ggatgacctc acccggcatg tcatgttttt
     1561 tgccaccacc ggtggcggta aaacagaaac cacctttgcc tggctgctca acccgctctg
     1621 ctggggaaga ggctttacct ttgttgacgg caaggcacag aacgatacca cgaggacaat
     1681 ctggtatctc tcccgacgtt ttggcaggga ggatgatatc gaagtcatca acttcatgaa
     1741 cggcggaaaa tcccgcagtg aagtcattca gagtggcgat aaatcccgcc cacagtcaaa
     1801 cacctggaac ccgttcgcat tcagcaccga agccttcacc gccgaaacca tgcagtccat
     1861 gctgccccag aacgttcagg gcggtgaatg gcagtcacgg gcgatagcca tgaacaaggc
     1921 actggtcttt ggcacaaaat tctggtgcgt ccgggaaggt aaaaccatgt ccctgcagat
     1981 gttacgggaa catatgacgc tggaagggat ggcaaaactc tattgccgtg gccttgatga
     2041 ccagtggccg gaagaagcca tcgcaccact gcgaaactac ctgcaggatg tcccgggatt
     2101 cgatatgtca ctggtgagaa cgccgtcagc ctggactgag gagccccgta aacagcatgc
     2161 ttatctctcc ggtcagttct cggaaacgtt cacaaccttt gccgaaacct tcggggacgt
     2221 ctttgcggca gatgccggcg acatcgatat tcgcgacagt attcacagcg accgcattct
     2281 gattgtgatg atccctgcac tggacacatc ggctcatacc acgtcggcac tcggtcgtat
     2341 gttcgtcacc cagcagagca tgctcctcgc ccgtgacctg ggctaccggc tggaaggcac
     2401 agatgcacag accctggaag tcaaaaaata caaaggcagc ttcccctata tctgtatcct
     2461 tgacgaagtc ggtgcctact acacagaccg cattgctgtg gaggccaccc aggtacgttc
     2521 actggagttt tccctgataa tgacggcgca ggatcaggag cgaattgaag ggcagacctc
     2581 cgccaccaac accgccacac tgatgcagaa cgccggcacc aaattcgccg gacgcattgt
     2641 cagtgacgac aaaacggcaa gaacggtgaa aaatgccgcc ggtgaagagg cacgggccag
     2701 aatgggcagc ctgcagcgcc acgatggtgt gatgggtgag tcctgggtgg acggtaacca
     2761 gatcaccatc cagatggaaa gcaaaatcga cgtgcaggat ttgatcagac tgaatgctgg
     2821 tgagttcttt accgttttcc agggggatgt ggtgccgtca gcctctgtgt atattccgga
     2881 cagcgagaaa agttgcgaca gtgatccggt ggtcatcaac cgctatattt ccgttgaagc
     2941 cccccggctg gagcgtctcc gccgtctcgt ccccaggaca gtgcagcgca ggctccccac
     3001 accggagcat gtcagcagca tcatcggtgt actgacagca aaaccatccc gtaaacgtcg
     3061 taaaaaccgt acagagcctt accgtatcat tgatacattc cagcgccagt tggcaacagc
     3121 gcagacatca ctggatctgc ttccgcaata tgatacagat atcgaatccc gggcaaacga
     3181 actgtggaaa aaggctgtgc acactatcaa taacacaacc agggaagaac gccgggtatg
     3241 ttatatcaca ctgaatcgtc cggaagagga acactccggt cctgaagata taccttcagt
     3301 gaaggcgatt ctgaatactc tgctccccct ggagatgctg ttacctgtac cggatctaac
     3361 tgcatccccg ccccataaaa aaaatgtcgc tcagacacca tcaggtggaa aacaagagag
     3421 tcgtaaaaaa agattctgat aacacctgta tggctgattt taataacaac ctttacgcga
     3481 ttattaatct cctccgctca ccatagtaag cggaggattc aggatttcaa tccatcacca
     3541 gaggtacaat aaatgtgtat tagtagcccg gaaacaaact catggtctgt tatttatcgt
     3601 aaaaattccg gagaagatat caatatcact tccctgacgt ttaaaaatag tcttctggca
     3661 gcccggacac tgatggtgcc cgaaaactat atgatatgca tactcagaaa cggggagagg
     3721 gtcagacgat gggacagaga aatacttgca ggctcaaaca gatggtacaa gtgttctccc
     3781 gataatttcg aaatactggg taagctcccc ataataaata aagtcacaaa actgataaaa
     3841 tcataatcta cggattttaa tcagttaagc taaatcagga tgaacgcttt gattatgaat
     3901 tatacatgaa aacaaatatg ggcagcacaa aaaatggtgc cagggttccc cacgttcgta
     3961 atagattcac ttctatggat attggtatga attcccagat cctgccagac atgctgatta
     4021 attttgccct tattaacatc acagacagaa agaacgaggg taccaatacc attgatggta
     4081 actggcaggc cgatgaagga cgccgttacc gggataatgt aagaatctat ttttgagtat
     4141 gtcaacataa cctgaggcaa agtacctcag gttccagtgt agtaatcagc caacctgaca
     4201 gtcttctgtt agccctacag atatgtcgtg gagctaaccg cgcatgtcaa agccggattt
     4261 ttgaaaatct ctataaactt ccggattgtt gaaggccggg aatttagctt tatgggctgc
     4321 tgactttatc gcttcgcaat aggctttatc gccattactg gttgatattt ttaacgccgt
     4381 gccatcccgg gagaattcca tatgcaacct gcattttttc cttttccatt tttgcggatc
     4441 atcaagttta gcattaattg catccctgat tccccgtgct tgctcccccc attcatcctg
     4501 atcatcccaa cgtcctgaac tgcaactacc cagggctgta gttttatggc aatttgaagg
     4561 atgtaatggc gcacatcccg ccacaaagct gactaaaata gccagcataa cgattttctt
     4621 tacccccata tattgctcct caatccatat agatctcagt accatagctc tgtcagagca
     4681 aagaaaatct taactaatac aagttaagat ttttaatatc cactcctcat ccctacattc
     4741 caggtgatgg tggcgtccac tcattatcct gaactggagc ataagatgcg ccatgccctc
     4801 cggatatgct tttctgttgc ctgatatatt cgtggtagta ctgttcacta cgctgacgat
     4861 gtaaatccac atccgggcgg ctgattacaa agtgtccatt tccggttatt ccagctgcat
     4921 tatgccaggc caccatttcc gcaaatacat cattaaactg ttgatatgag ccttccggtt
     4981 caaaacccat tcctggctca cgatcaaaca atacaggagc aatcagattc agcttatcca
     5041 ccagttcatt tctgtcctga tgatggtaga acaccacacg gtccccataa tccacacaga
     5101 ctaactcgcc attcctccga tcacgaaaac gcacactgcc atctttctgc agacgggctt
     5161 cgagaacgcc agtgtcttca taaagtggag ttcctcccgg ttcacagaga atcacgcagc
     5221 ggtcggcatt cgttgttcgt cgcttgtctt tgcgacgatc acgataatcc cagccacgta
     5281 actgtgataa tgcagctctg tctccctgta ccgcctgttg ctccacccac tgccggtagg
     5341 acaaaggata ccacttaccc tccgcaatca gtgacaaccg ctcttccttc acactctcct
     5401 tcagcctgat cagcgcctgc atacgctgca cttctgcaat atggtaatgc agcttacgca
     5461 attgtggatc ccggaactgc acacggatat acgccttgcg ccgacggcag gcagcatgaa
     5521 tttcccgtaa ccgttctccg taacgtaagt cgggtttacg ccagtgctcc ttccatgcaa
     5581 gataacgcgc gcgcaaatct tcccgcgcag ctgcacgggc ttcacggcgt tcacgacgca
     5641 gtacaggatc gcgccggaag ccgggctcca cttcatcgct ggcagcaagt tccggattgt
     5701 agcgacattc aggcttcacc cgttcaaata tatctgcacc tgcaatctca aatggccctg
     5761 cctgcggctc agccctggat aacgtcaaat ccgggtggat actgctggcc ttcactggtg
     5821 tgagctcatg attaaacgca tccacaatca caagaccatg atgctgtttc tgcaacataa
     5881 gccctttacg cgcaaacatc tcatggcacg cctgccagct gaaattcccg cccgcgatca
     5941 tatcttcaat caacgactca cactgaacag tcgtgtaccc ccacagactt tccttatcag
     6001 aaaacagttc gcgctttatt gctccctgtg gacctgtccc catccgcata cgataccatt
     6061 cgtaatcact gacagcctga gtaccaagat tacgtccccg gaatcgttct ccgggtataa
     6121 cctgagtaaa aatatccttc ggtacaggct gccatccacc atccagatcc tgcatataat
     6181 ccagcgtcat taacggccac acactgtctg cacgtactgg tgtctgttgt ttatcaaatc
     6241 catcatgcag taccagatgc ccgtgctgga tccccatcca taatccagcc tttgccagag
     6301 ttctgtgcac atccaggcaa ctttccagct caccatttct cgccatttcc ggtaagcgtt
     6361 cagcaaagtg acgacaggcg tactgtttca gactctccgt ttctgccact ttttccggcc
     6421 ggatatccac gttaatggct tccggctcat aacgaccggg tgtccccacc tgactgaaaa
     6481 tatctgtcgg tacaggctga tattccccca gtttccgccc cagtttttca gctgtccatg
     6541 acggtccgaa cgaactgagt gcaacacctt cccgcgctct gtcccagcca tccttcacca
     6601 ccagttcccc ttcctgcatc gtgatataca gaccatcact ggccagccgt ttatgcaaag
     6661 ataaccagtc ctgtgcaggc tcttcacgca gcccggcaat cgacatctga gcaatatatt
     6721 ccctgaacgt ctgctttttc ccgcgtcgcc aggcattacg gcgctcacgt accagcgctg
     6781 ttttgcgcac aatacggttg ccgggtgcat gcacaaagca gccattatca ggcgcgaatc
     6841 cgtgcttaag ttcaagttca cgacatgcac ggctgagcct ttcctgactc caggaaagac
     6901 gattaatatg accactaaca ggatgaacac gattcactgc gacatgtaca tgcaggttat
     6961 ccgtgtccgt atgaacggca gccacgtact gatgttttga gagttcaagg gatttcaatg
     7021 tgtgacgcac acagtcaaaa agctgctcag gacgcgggct ttcatgcgca ggccaggaga
     7081 gaatgtagtg gaaaacgggg tccgtatcat tacgggaaaa aacagctttg tccgccttgt
     7141 actgcatctc ttcagcagct gtttccagtg aattgcagtt atggaaacag gtaacgccat
     7201 agaaatttac ccactgactg ccgtctttca tcacgtcaat cagggagaca aatttttcgt
     7261 tacgcaggcg tgtagcatag tcgacaaggc gtctgaaacg gttgcggtga gggacgtcac
     7321 tcttgatttc gctgtcttct gtgagctcat tttcctgcat atcatcccgc acagatgtgt
     7381 acgcaatcag gtcttcaaac gaagacctgc cgtcacgtct tttgtgcgga ataatggcat
     7441 tcatcagcag ttatctcagc gtcccattaa gtctcttcgc agagcattga catattcagt
     7501 aatggcaaca agaacctcgg catactcctt atcaccagta cgttttcctt ccacaaacag
     7561 atgcttctga aggcggccaa gacgcataat ctcatcaatc atacggtcgt cagtacggca
     7621 gttaaccacc ttatccagtg aagagcttcg tataaatgtt gaaaccgtct gtccacttga
     7681 ctccgctttt tctctgacga tttcagcctc atcctccgtg aatctcgccg tgaattttac
     7741 aattctcttc ctgttctcac tgcccgttcg ggttttcttc tcactcatgg tctgctcctt
     7801 accggggtgt cggggcgaag ccctgaccag gaggcatttg tctgattgcg cgtgcgcggt
     7861 ccgacaaatg cacatcctgt cccgtttttc aggacattat agcctgttat ttttgtgcat
     7921 catagtctta tgcagacggc agaggcaggg cgcagccctg ccaggagtca gtttttgatg
     7981 ataaaatcag gcttcaacat gaaacagaga gaaagtgcca atggcttcta aaaaatttta
     8041 ctcagatgat gacatccagc tggcaaaagc tgctttatcg gaattgccag atctcaccgc
     8101 tcaacggaaa acattgcgtg attttcttga tgccattcgt gatgacatta ttgtcctggt
     8161 aaggacaaag ggttatacac tggcagatgt tcgtgacacg ctcaaaaatg cagggtatga
     8221 agtaggagag aaggcacttc gtgacattat tcgggaagca gaatcgaaaa aaccatcccg
     8281 ccgttcttcc tctaaaagag cacctaaaaa gacagattca ggcaggaatg atggcatcga
     8341 tatgaacaac aattgatgtg acaatgtcac atcgtgttct gagattatga tgattac
//
LOCUS       DAZKGF010000192         8289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZKGF010000192 DAZKGF010000000
VERSION     DAZKGF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.192"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..390)
                     /locus_tag="ACHOQV_004340"
     CDS             complement(<1..390)
                     /locus_tag="ACHOQV_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632622.1"
                     /translation="MIRIIVFLLFFLSGCTSQKEYSNIVRIENRCSSDIDVFIQGYVS
                     GSTLIYQHNIHLPVGDSDVLVYFSTADIDDGFKYEGAFMIYKDSRGELDYPFTISLSG
                     REKKFGKNDLIRLSRDISTKDDRKYGVI"
     gene            complement(383..1789)
                     /locus_tag="ACHOQV_004341"
     CDS             complement(383..1789)
                     /locus_tag="ACHOQV_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFP9632623.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDF
                     TITNKGDEAEALSLEVLDGNEVIYSERQTGKYCDKGEHAWQWDGYSNQGILDTTKIKS
                     KSLLVRLIALCGDMMIKVDYPLKNAAKEEEWVDVKVDRNNKVVDITWRLSVKDSGTKG
                     SNPELSPIPYSDLVSLTKNGVEFYWSRNGSRGGGIGGNIVTAIGVFKVNVKAEINITP
                     SMRTFDLISSLDPDMQSSVSLLGFEKIYHNYGGYYNNSKDEIKALLYSNERYKWDSAH
                     EMGHKVMDKYGSESWTHKGTSTRGQSTIPNTPMPSSGEIDIMKYADSYAPDRYKRLVA
                     ADEDVQALIWLSRVSFND"
     gene            complement(1795..3405)
                     /gene="tssA"
                     /locus_tag="ACHOQV_004342"
     CDS             complement(1795..3405)
                     /gene="tssA"
                     /locus_tag="ACHOQV_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP9632624.1"
                     /translation="MSELLQKLTRSCFADRDALDSARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGAQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLTVSFTAWPEDQHSPSLMPLINALESRLAQSGRMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADELTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDIAAQGLTLTQWEPDLLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVTIDPVRAAVLCDTQHKD"
     gene            complement(3398..6724)
                     /locus_tag="ACHOQV_004343"
     CDS             complement(3398..6724)
                     /locus_tag="ACHOQV_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFP9632625.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQSFELHRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSTEPDVALLTALKK
                     LRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQT
                     GRPLQSVGCLLSERCTPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAER
                     GIAHWQSILKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSPVWAGVTGDNAR
                     GRTVGFPWLRITLMSAVCVLAIWGAGMTTSFFANRALVQETSIQTARALDIRLPLSEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADDLRY
                     AGVPEGVRQSVLPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARREEIDWVLSDRQQDTSADISPDMLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDP
                     QEMTQQLAQTVLQGKTVDLTDTRDYGSLIAASLGEEWSGFGQALFVRPVEQSWRQVLT
                     PAADSLNRQWQRAIVSHWDQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANL
                     SGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKPA
                     RDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGS
                     WSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQ
                     VFLTGRSMKDAEEYGEDADE"
     gene            complement(6781..7995)
                     /locus_tag="ACHOQV_004344"
     CDS             complement(6781..7995)
                     /locus_tag="ACHOQV_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632626.1"
                     /translation="MPIRLNTIPDAEPYPAPPARIKWLSALLLMLSVGVAITSLFASD
                     ELAKNGPYFWGLACGVPAFIWSLVGSVRWLVFVTQHIRADAWNQRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETTTAFLARHQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFIQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGMWTVELNREQRASLLSS
                     NQPFTQEALMYELDAFLGCSGPAAPWLSVAAATLAAIQSQHPQLTLSGVQNGHYSWAT
                     MVSPFVSPQEAS"
     gene            complement(8101..>8289)
                     /locus_tag="ACHOQV_004345"
     CDS             complement(8101..>8289)
                     /locus_tag="ACHOQV_004345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632627.1"
                     /translation="NRMYYWLPCEIRGRLMYDGIEWSFVINAAATAMWMSNDKEIYWG
                     CNKKACDNFFLLPYDGMR"
BASE COUNT         1771 a         2421 c         2114 g         1983 t
ORIGIN      
        1 tataactccg tattttctgt cgtcttttgt tgaaatatct cgtgataatc taattaagtc
       61 attcttccca aatttttttt cgcgccctga caatgaaatg gtgaatggat aatccaattc
      121 accgcggcta tctttatata tcatgaatgc cccctcatat ttaaaaccat catcaatatc
      181 tgccgtggaa aaatatacta atacatcact atcaccaact ggcaagtgta tattatgctg
      241 ataaatcagc gtagaaccgg aaacatatcc ctgaataaag acatcgatat ctgaggagca
      301 tctattctca atcctgacta tattggaata ctctttttgt gaagtacacc cactcaaaaa
      361 aaacaataaa aaaacaatta ttctaatcat taaaactcac cctgctcaac cagatcaatg
      421 cctgaacatc ttcatctgcc gcgaccaatc tcttatatct atcaggtgca tagctatctg
      481 catatttcat tatatcaatc tctcctgaag atggcattgg agtattaggg atggtacttt
      541 gaccccgcgt ggatgtcccc ttatgtgtcc atgattcaga accatattta tccataactt
      601 tatgtcccat ctcatgagca ctatcccatt tatatctttc attactatat agaagagcct
      661 ttatttcatc cttgctgtta ttataatagc caccatagtt atggtatatt ttttcaaacc
      721 ccaaaagact taccgacgac tgcatatctg gatctaacga tgatatcaaa tcaaaagttc
      781 gcatagatgg agtaatattt atctcagcct ttacatttac tttaaaaacc ccaatcgctg
      841 taacgatatt accaccaatt cccccgccgc gacttccatt tcgtgaccag taaaactcca
      901 cgccattttt agtaagacta actaaatcgc tataaggtat tggcgataac tcagggttac
      961 tgccttttgt accgctatcc ttaacagata atctccatgt tatatcgacc actttattat
     1021 ttctgtcaac ttttacatca acccactctt cttctttcgc agcatttttc aatggataat
     1081 caacttttat catcatatca ccacataaag caatcagtct tacgagtagt gatttgcttt
     1141 ttatcttagt tgtatccagt atgccttgat tactgtagcc atcccattgc caggcatgct
     1201 ctcccttatc acaatattta ccggtctgcc gttcactata aatcacttca ttaccatcaa
     1261 gcacttccag agaaagcgct tcagcctcat cgcctttatt tgtgatggta aaatctattt
     1321 tttctgtatt cttcgtacta ccaccgctaa tcatttcttt ataatcaggt atattcagtg
     1381 gcgtcagtat cttattacct gataatttaa ttctgtaatc cagttttaca tcatcagagg
     1441 atgaatcaga accagaagtc agatcctttt tcgccgactg cgcatgttgt tccgcttcac
     1501 tttccccgtc atccgtctca tacgtatcat ccatcattga cgcaataaac cgtgccttac
     1561 acgggcaact gctctgactg tccagcgtcc ccgccatcag cctgccgtga atatctgttt
     1621 caggaacgcc gcccacaata ataaacattc ccggatatct gccgcaggtg acccggtcca
     1681 tatcccgcgc ctgcgggata cccatgatcg tatggtcgtc cgcgccttca ataattttgc
     1741 cgccgcaggt ggttttgtcc cccctgacca gaaagtagcc tgttcccatt gccatcagtc
     1801 cttatgttgc gtatcacaca ataccgctgc ccgtaccgga tctattgtca ccagcgttcc
     1861 cagcaggatt tccatttttc ggttcagcag ggctttatcc gcatagcggt gcgcccgcag
     1921 gcgcagcagt ttcagctgtc gggcttttac ctcaaacagc aggtccggct cccactgcgt
     1981 cagcgtcagc ccctgtgcgg cgatatccag ttcttccagc agcaggagcg ccatctcgtt
     2041 tttaccgtac tgttccgcca cccgcgccat cagcagtcgc aataacaggc gctggcgtgc
     2101 cgtggtgatg cccggacggg tctgtatcca gcccagcgcc gcttccacgc cctgactgtc
     2161 tgccatctcc agcgcttccg gctccagcgc cagtacgtcg tttgtggcgt cgtctgtcgg
     2221 tacagcggct ttccccgcaa gccatgcgcc gtcttcccgc atcatgacct gctgcgctat
     2281 ccagttgcgc gtcaactcat cggcgaacgg cgtgccgtcg ttccatgcca gattttccag
     2341 ccccggcagg cgttccagta gtaacgccag gtcctgacgg ataaccgctg tccacctgtc
     2401 ccacggtgcg cccgccttag tcagcgcctg atgcagatac cactgaatgt ccagccagaa
     2461 atggctgacg ccggtgctga acatcaggtc ggcctgctcc agcagttccg tccagttctg
     2521 ctgagcatac agccgcttaa gctggttgcg tgattctgtg cgcggcgggg ccagccgggt
     2581 ttttccgtcc acatccggcg gcagttcatg caccgtgtcc cagcgcaggg ttttcatcag
     2641 ccggtgtgct gaaagccagc cctgcggctg ttcgttcaga taacgggcca gcactttcgc
     2701 ctggtcgagc aggtcgcgcc cggaagtgat ggtctgtacc tgtggcgcat ccaccgggga
     2761 agacggtgcc ggaacgccgc tgctgttctg cggcaccacc gcgttcatcc tacccgactg
     2821 cgccagacgt gattccagcg cgttaatcag cggcatcagg gagggggaat gctggtcttc
     2881 gggccatgca gtgaaggaaa ccgtcagttg attaagagcg gcaacaatgt tcgcaaaatc
     2941 ctcttttgcc acttccgggt agcgggcaag gctgtcgagc atcttttccc ctgccagcca
     3001 ctccagcgcc atttttcggc ttgccgggcg cgagggcagc agttgcgcac cgaagcgttc
     3061 caccagtccg gctaacagcg ccagcccctc agccagtccg cgttcgccat cgcggtgcag
     3121 tcgcgcccag atatagtaag tggcgatgcg cacgtctttt gcctgcgtca gcagcaggga
     3181 ttcggcaagc tggcagataa ggtccgtatc cgcgccggaa agtttgttca tctcgtcgcg
     3241 gatacgcagg aaatcgtcgt ggtagccggg gtcttccccg accggcgtat ctgccgtcac
     3301 cggcagcagc catgtctgcc acagcgccgc ctgtgttcgc gccgagtcca gtgcgtcccg
     3361 gtcggcaaaa cagctacggg tcagcttttg caacagttca ctcatcggcg tcttccccgt
     3421 attcttccgc atccttcata ctcctgccgg tcaggaacac ctgtcccggc aggcggaagt
     3481 ttttcagttc cagcagcgcc agcggccctt taccctgttc aacccgcagc aggtaattca
     3541 gcggcaggcc gtcctgcgct ttccacacca ctttaaaggt actgctgtcc accggtgtca
     3601 cctgcgcctg ttccagcagg cgaataaggc tccagcttcc ccgataatcc gccagtatgc
     3661 gttccatcgc ctgcgtactg gtccagctca gcgatgcgcc gggttgccac tgttcatccg
     3721 gccagttaaa acgctgccag cgttccttct ggttgaaata ttccagctcc tgcccgtcaa
     3781 tcaccagatg cgtcttcatc acgtcgcgcg ccggttttgc ccgcagttca aaatgtatcc
     3841 ctgcatcgcc gctggcaaag gcggtatcgg ccacatcgcg caggcggttc agggcgcgaa
     3901 taaagtccgg attgactgtc agcccctgcg tgttcatggc gtcagccacc cagtagcggc
     3961 cttcacgttt cagtacgccg gaaagattgg cggcgataaa ctggttgatg cgcccgtcat
     4021 cgcgcaggta ctgcgccagc agggggaggg aggcatcgtt ttgtgaggct ttgaacgggt
     4081 agcgaccagc gaagtcctga tcccagtggc tgacaatcgc ccgctgccac tggcggttca
     4141 ggctgtctgc cgcaggcgtc agcacctgcc gccatgactg ctccaccggg cggacgaaca
     4201 gcgcctgacc gaagccactc cactcttcac ccagactggc ggctatcagg ctgccatagt
     4261 cgcgggtgtc ggtaaggtca acggttttgc cttgtaagac cgtctgcgcc agttgctggg
     4321 tcatctcctg cgggtcaggg gcgttggtca cctgttgcag tttgaggcgc acgcgggtga
     4381 ctcgggtgag gtaggtctgc aggctcagct ggctgtcgcc gcctcctgcc ttatcgccaa
     4441 gcaaccgcag cagcggcgcg aaggttttat ccagcggccc gacaggcacg tcgccttcac
     4501 gggattgctg cggcgttttc tccttgccgt taaacagttc ctgtgccgat ttcgccagcg
     4561 agtctgacag gccacggttt tccctgccag ccgccgcctg ccacgcgagg gtgtctgtca
     4621 gcgcaatcag cggtgactgg cgggcatcgg ccatcagcgt caactggtcg agaatgccgg
     4681 agagcgagtc ttcctttttc cagcgaatgc tgttgagaaa cgccagccag cttccggcaa
     4741 agtcggtaaa gtagcgtgaa gtgagacggt tacgcagcat atccggcgag atatccgcag
     4801 aggtatcctg ctggcggtcg ctgagtaccc agtcgatttc ctcgcgccgc gccgtcacca
     4861 cctgctcgat ggcttccctg acctgtcctt cccacgcctg acgggtaaac atccccggca
     4921 ccgtctgctc cgtactgaaa agagattcgg tgagcgtatc cccggtcatg tccgccagcg
     4981 tcatatcggc gtagttgcgg gacacctgtt gcagtacgtt ctggtagaga gtgttttcgg
     5041 cattgcgcac accaatctgg cgcagcagga ttttgcgcac tgcgccggtc agttccggtg
     5101 gcggcgaagt tttccactgt gggtgttccg gcagattcgc catccagaag gtcagcagtg
     5161 acggcaacac gctctgacgc acaccttccg gtacacctgc atagcgcagg tcatctgcca
     5221 tcagcgtggt actgaaaaag gcagcgtcgg ccttttccgg gcgggaagtc atcagcaatg
     5281 ccttaagctg cttatagcgc tgttcgccgg tggcggtacg ctgcggacta ttgggcggca
     5341 gcgcgacaaa ggcattcagt tgctgttgca gatggtcaac ggccacgtcg cgcaccagcc
     5401 ggtttgccgc ctgcgcatag ccgggaaagg cggcggcgag cagttgctgg ttacgttcaa
     5461 ggccaaaacg ctgataccac ggcgcaccct cccggatacg gtactgcagg cgctccagtt
     5521 caccctgcag ggtatgcagc gccaccagtt gttcagacag cggcagacgg atatcgagtg
     5581 cacgcgccgt ctggatgctg gtttcctgaa caagtgcgcg gttggcgaag aacgaggtcg
     5641 tcattccggc cccccatatc gccagtacgc agacagcaga catcagcgtg atacgcagcc
     5701 acggaaagcc caccgtgcga ccccgcgcgt tatcgccggt cacgcccgcc cagaccggtg
     5761 acggcagcca cgcatgaggt gcggcttccg gtacggcggt aagcggcggg ctgaacatca
     5821 ggccacgcag gcgcagggag gaaaatgcgc cgcctgccag cagcggtttc aggatacttt
     5881 gccagtgagc gataccccgc tctgcaaggg tatgggccag acgcagcagg aaatcatggc
     5941 ggttatcggt aagcagctgc gacatgccct gttccgtcag cggcagggtt tgtgcttcca
     6001 gcattgcagc cagttgttcc ggggtacagc gttccgacag caggcagccg acgctttgca
     6061 gggggcgtcc ggtctgataa tcaccgttgt cacagacctg ccacaaatag agtggagcct
     6121 gaaaaccgag tcgcttaccg ccgtttatca gttcgcgcca gcctttgtcg agttgcgccg
     6181 tctggcggct ctgttcttct gtcagcggcc agatgatgcc gtccagcgga cggctgcggc
     6241 gcagtttttt taaggcggtc agtagcgcga catcaggctc tgtagatggc ctgccgccat
     6301 atatcagcac cgtgtggtcg ccctccagcc agtgttgccc ggtcagcccc ggcgcgatgg
     6361 cctctgcctg ttcaggctcg ccggtcacca gcagcaggcg gattttacgc cgccagaatg
     6421 ggccgtagcg gtcgcgcagg aaggcggcga gttccaccat tgccggatga cggctgtcgg
     6481 ctttctttgt cgtcaccgcc cgatagtcat ctccggcggc aagctgtctg tggagttcaa
     6541 agctctgtac ggcagcgtgg cggttcagca gatgataaac ccccatgata atcagaaaga
     6601 ccacacctgc ggcaatcacg ccagccatcc agtacatttg ctgtgaacta cccggttcaa
     6661 cgcccagcaa tgccggttta cggtgaataa acgtgcaaag cgcaaccaca acggccacgg
     6721 tcatgacgat ggcaggccct gaaaccgaac ggggccggag acggtttaat cccggaacca
     6781 ctatgatgcc tcctgagggg aaacgaatgg cgacaccatg gtcgcccatg aataatgccc
     6841 gttctgaacg ccgctgagcg tcagttgggg gtgttgtgat tgtattgccg ccagcgttgc
     6901 cgcagcgacg gacagccacg gcgctgccgg tccggagcac cccagaaagg catccagctc
     6961 gtacatcagg gcttcctgag taaacggctg gttagatgac agcagcgagg cgcgctgttc
     7021 cctgttcagt tcgactgtcc acatgccgga tatggcatct ggctgcaccg gcatccagtc
     7081 cagcgcctgt gcaatgcctg acgccatcat ctccgtatct gtgatacgtt caggacgatg
     7141 cagcagggcc agcggcgtca gcgcggtttg cgtcagtcgg tttgccagca acagcgcggt
     7201 ggcggattct gccgtccgtt ccgggttctt cggttccaga cgcacggaaa tcaccagcag
     7261 tacggccttt tcctgaataa agttgtccag ccagtcgtcc acggcggcca gccccggctc
     7321 cggggcagaa aacagacggg cttcggggag tccggctttt tgccattcgt cctgccagat
     7381 agttctcacg gaggcccctg aaagcggcgc atcaatttcc agtagtacgt tgatatgaaa
     7441 atcagggggc agcagaaaca attgcggtct gatatcaata aaaaggcggg aaataatttc
     7501 ggataaacgc ccggtaaccg gtttattggt taatacggga atgacactgc gacgcacggt
     7561 atcttctccg cgcaaatcag cgtaagtatt caacacctga tgacgcgcca gaaatgctgt
     7621 tgtagtttct gcgacggaat caccattcag cgcggtttgt acggaaaaac tgagtatctg
     7681 caacgcccgt cgtccccgcc gtgtttcctg caaaatgact tcttcgcgac gttgattcca
     7741 ggcatcggca cgaatatgtt gagtgacaaa taccagccag cgcaccgaac cgaccagtga
     7801 ccagataaaa gcaggcacgc cgcaggccag cccccagaaa tatgggccgt tttttgccag
     7861 ttcatcgctg gcgaataatg aggttattgc cacaccaaca gacagcatca gtaataaggc
     7921 tgacagccat ttaatgcggg ctggcggtgc gggatagggt tctgcgtcgg gaatggtatt
     7981 aagtcggatg ggcattgtaa taatccgtta ttctgaaata tgaatacaat gaatctcaag
     8041 gtgcttgaac tatctgatta attcacttgt ccataatgtt tttgctttat taactgtgat
     8101 ttacctcatc ccatcatatg gtaataaaaa gaaattatcg catgcttttt tattgcatcc
     8161 ccagtaaatc tctttatcat tgctcatcca cattgctgtt gcagcagcat tgataacaaa
     8221 actccattct attccgtcat acattagtct ccctctaatt tcacaaggta accaataata
     8281 cattctgtt
//
LOCUS       DAZKGF010000193         8286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZKGF010000193 DAZKGF010000000
VERSION     DAZKGF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.193"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            164..1720
                     /locus_tag="ACHOQV_004346"
     CDS             164..1720
                     /locus_tag="ACHOQV_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP9632628.1"
                     /translation="MHQYLLFLLGLTPLLAMIILILKVKMPIHYAVLSTLFITLILAT
                     LFWHTPVQDLGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMNILRNVLAAVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLAAFNILLPFVIVAIIGEGLKAIRGVGFMTL
                     MCGVSTLIPQYLVAVYLGAELPAFAGSLVSLIVIVFMAKNRKEKTAPEFLIETSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTVKSTVSQIASSLSFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGWIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            1770..2489
                     /locus_tag="ACHOQV_004347"
     CDS             1770..2489
                     /locus_tag="ACHOQV_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP9632629.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQNSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGARLNGRAVYHPSCSLSRKLGVTREPLLLLENVRGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLSREGHPVKVMHIAEVLMHR"
     gene            2500..3915
                     /locus_tag="ACHOQV_004348"
     CDS             2500..3915
                     /locus_tag="ACHOQV_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP9632630.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSLAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREEATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNGVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFREVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEVLGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKELPYACSLCTACNGVCPVKIPLAQLIQKHRQVMAES
                     EITPSTERRITRIFNYVNSHPALWKVGMIAGAKTASLCIRNGKTPISPGAIKEWSVAR
                     DLPSPDGESFRSWFKRHKKGG"
     gene            3920..4615
                     /locus_tag="ACHOQV_004349"
     CDS             3920..4615
                     /locus_tag="ACHOQV_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP9632631.1"
                     /translation="MENREEFLVAIARKLGRPVRRIPEAMPEPVSTLATTRLTELTMD
                     QRCEAFIKFASEVMLAKCVLVSPEDAPSKALDICWTFGSGPVIISNDQRLFDTGIAPL
                     LQKEMGAVLWTPEKGEENIHLAEKAKTGVVFAEYGLTESGGVVLFSAPERGRSVSLLP
                     EASVFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDC"
     gene            complement(4751..7912)
                     /locus_tag="ACHOQV_004350"
     CDS             complement(4751..7912)
                     /locus_tag="ACHOQV_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFP9632632.1"
                     /translation="MILQTHTIAETSNFIVLDKYIKAEQTGDSYQSESDLERELIQDL
                     QNQGYEFISVKSQSAMLANVREQLQSLNGVVFNDSEWRRFTEQYLDNPGDGILDKTRK
                     IHIDYICDFIFDNGRLENIYLIDKKNLMRNKVQIIRQFEQAGSHSNRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQLHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQ
                     IAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFRRYYQFGFTGTPIFPENASGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSRGFNAMLAVSSVDAAKVYYATFKRLQEEAAN
                     KSANYKPLRIATIFSFAANEEQNAIGEISDETFDTGAMDSSAKEFLDAAIREYNDHFQ
                     MNFSTDSKDFQNYYRDLAQRVKNQDVDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRNLEQATIDAITLFGDKNTRNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMAVVSELEQRFPDPASIESEKEKKDFVKLFGEYLRAENVLQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKFAELQMIRLPAERKIQDYRSAYNDIRDW
                     QHREKEADKKEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHSRQNKSKDEMTVEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDTFFTFAQREQQREADALIKEE
                     NLNEEAARRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQTVFQKISAFIEK
                     FKGVGGASLVTGAQTPPCLNKQA"
     gene            complement(8032..>8286)
                     /locus_tag="ACHOQV_004351"
     CDS             complement(8032..>8286)
                     /locus_tag="ACHOQV_004351"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9632633.1"
                     /translation="FTYIQKASVSSSVTSLRRPMFLKFPIPVPPSEEQARIVEILDKF
                     DTLTNSITEGLPREIELRQKQYEYYRDLLFSFPKPETVSN"
BASE COUNT         2104 a         1812 c         1980 g         2390 t
ORIGIN      
        1 attataaccc ccttccctgt ttgtggacga gactcgttct ctccctgcga gacaagcgta
       61 aattggtcac accgacggtc gttgcctgaa aattacaaat tctcgcctga cagggcaata
      121 acaacagtaa tgatgctgaa tttgttttcg ttgaggaaat gacatgcacc aatacctgct
      181 ttttttactg ggacttaccc cacttctggc aatgatcatt ttgattctga aagtcaaaat
      241 gcccatacat tatgccgtac tcagcacact ctttattaca ctgattcttg caactttgtt
      301 ctggcacaca ccagttcagg atctgggttc tgctgttgta tatggcgcaa tcaaagggct
      361 gtggccgata gtgatagtca tacttgctgc catttacagc tataacctga tgaatgcgac
      421 aaaaagcatg aacattctgc gaaatgttct ggccgctgtc agtgatgata aacgtatcca
      481 ggtgcttctt atctcatggt gttttggtgg atttcttgag gcggcggccg ggtacggtac
      541 agctgtagca atccccattg gtatactcat tgcactgggc tttaaccctc ttaaagccgc
      601 aattgcatca ttggttgcta atacagtacc taccgcgttt ggtgctgtcg gcatacctgt
      661 atcagtactt gctgaacagg taaatttacc cgtaacaacc ctcagcggga cgattattct
      721 tcagctggca gcgttcaaca ttctgcttcc cttcgttata gtcgctatta ttggggaagg
      781 cctgaaggct atacgaggcg ttgggttcat gaccttaatg tgcggtgtga gcacattaat
      841 cccacagtac ctggtggctg tttatcttgg cgcagaactt cctgctttcg cgggaagtct
      901 ggtcagcctg atcgtgatag tctttatggc aaaaaaccgt aaagaaaaaa cagctcctga
      961 atttcttatt gagacttcag cagatagcgc cagcgcactt tcatactctc gtggggcgtt
     1021 gttacgtgcg tgctcaatct acatcctgat ttttctgttc attcttcttt gctctccact
     1081 gttcccgacg gtaaaaagta cagtgtctca gattgcctcg tcactcagct tctctctgag
     1141 taacggcagc aatctgtcga tgaagattga atggatcgca acccccggtg ttctgattat
     1201 cattgccagt gttattggcg gctggataca gaaagcatcc ctgtcatgcc tgttaaatgt
     1261 atttgtcagt acggtaaaac agctcaaaaa ctccattatt gccatttctg cgattgttgc
     1321 tatggcaacg gtaatggatg tttgtgggtt gattggcact ctggcacaga gccttgtaga
     1381 tctcactggt ggggcctacc tcttcattgc tccggttatc ggcgctcttg gtacatttgt
     1441 tactggcagt gacacaaact ccaacattct gtttggtaaa ttgcagacca ctgcggctga
     1501 aaaattgcat gtggacccac tctggctggc tgccgctaac acatctggcg caacaggcgg
     1561 caaaatgatt tctcctcaga gtattgctat cgctgtttca gccacccgta tggatggtca
     1621 ggggagcgta atcatgtcag ggacactgaa gtactgcttt gcttatattg ttattcttgg
     1681 actgaaagta ggccttgtct accacctgtt catggcctga gaaaaaataa caaacggacg
     1741 tggaatatta atcaacagga gaaatatctg tgaatgttaa ctttttcgtc acctgcattg
     1801 gtgatgcatt taaatcccgt atggcacaaa acagcgtgct gttactggag caattgggtt
     1861 gcaatgtcca tttccccgaa aaacagggat gttgcggtca gccagcgata aatggcggtt
     1921 atgttcgtga tgccattcct ggtatgaaaa atctcatatc ttcgctggaa gagaatgact
     1981 ggccggtagt atctcctgcc gggtcatgca cttacacgat aaaaaattac gctcgctatc
     2041 tcagtgaaga acctgagtgg gcggcacgag ctgaaaaagt agctgaacgc ctgcatgatt
     2101 tgacgtcatt tatagtaaat catcttggca tcacagatgt tggggcaaga ctcaatggtc
     2161 gcgcagtgta ccatccttca tgcagccttt cccgtaaact gggtgtaacc cgagaaccgc
     2221 ttttgttgct tgagaatgta cggggacttg agctgctgcc gtttcacgct caggaaacgt
     2281 gctgcgggtt tggcggcacg ttctctgtaa aaatggcgga gatctctggt gaaatggtga
     2341 aagagaaagt cgggcatatt atggatgtta aacctgattt tcttatcggg gctgacgcca
     2401 gttgcctgct caacatcggc ggtcggctta gccgtgaagg gcatcctgta aaagtgatgc
     2461 atattgctga agtcctgatg cacagatgag gtaaacagaa tgtctttgaa aacaagtgat
     2521 atcaggttta aagagcgtat ccgtattcag attcaggacc cgatcatgcg aaaagctgtg
     2581 gctaacgcac aggagcgcat agggactaat cgacagaaaa tggttgatga actggggcac
     2641 tgggaagaat ggcgttcact tgctgaacag attcgtcggc atgttctgga aaatcttgat
     2701 gcttatttgt atcaattgtc cgaaaaagtt actgcgaatg gcggacatgt atattttgcc
     2761 cgtaccagag aggaggcaac cagttacata ctgaaggtgg cgaaagaaaa aaacgccagg
     2821 aaggttgtta aatccaaatc aatggtgact gaggaaattg gtatgaacgg cgttcttcag
     2881 aaagccggga taccagtgat agaaacagat cttggtgaat atattcttca gctggcgcat
     2941 gagccgccat cgcatgttgt tgtgccggca atacataaag atcgccatca aattcgcaga
     3001 gttttgcatg aacatctggg gtatgacggc cctgaaactc cagaagcaat gacattgttc
     3061 atccgtgaga aaatcagaca ggattttctg agtgctgaag ttggcgtaac agggtgtaac
     3121 tttgccgttg cagaaacagg ctcagtttgc cttgtgacaa acgaaggtaa cgcacgtatg
     3181 tgcaccacgt tacctaaaac acatattgct gtgatgggta tggagcgtat tgcaccaaca
     3241 ttccgggagg tggatgttct tatcaccatg cttgcaagga gtgctgtggg agcaagactg
     3301 acaggttata acacctggct tacaggtcca agagaagaag gacatgttga cggcccggaa
     3361 gagttccatc tcgtcattgt tgacaatggg cgttcagagg ttctcggctc agaattcagc
     3421 gatgtattgc gctgtattcg ctgcggtgcc tgtattaata cctgtcccgc ttatcgccag
     3481 attggtggac atggctatgg ctcaatctac ccgggcccaa tcggagcagt tatttccccg
     3541 ttgcttggtg gatatgaaga ctttaaggaa ttaccttatg cctgctcatt gtgcactgcc
     3601 tgcaatgggg tatgtccggt aaaaatacct ctggcacaac taatacaaaa acatcggcag
     3661 gttatggcag agtccgaaat aacaccatca acagaacgcc gtataaccag aatattcaat
     3721 tacgtaaaca gtcatccggc attgtggaaa gttggcatga ttgctggcgc gaaaacagcc
     3781 agcctgtgca tcaggaacgg aaaaacgccg attagcccag gtgctattaa agaatggtct
     3841 gtggccagag acttaccttc tcctgatgga gaaagcttcc gtagttggtt caaaagacat
     3901 aaaaaaggag ggtaacttta tggaaaacag agaggaattt ctcgttgcta tcgccagaaa
     3961 gcttggcagg ccagtaagac gtattcccga agcaatgccg gagcctgtaa gtacgctggc
     4021 tacgacacgt ttaaccgagt tgacaatgga ccaacgctgt gaggctttta ttaaatttgc
     4081 ctcagaagtg atgcttgcga agtgcgttct ggtttcgcct gaagacgcac catcaaaagc
     4141 attagacatc tgttggacgt tcggttccgg gccagtcatt atcagcaacg atcaacgact
     4201 gtttgatacg gggattgcac cattgctaca aaaagagatg ggcgctgtcc tgtggacccc
     4261 ggagaagggt gaggaaaata ttcatcttgc agaaaaagca aaaacaggcg tcgttttcgc
     4321 tgagtatgga ctgacagaat ctggtggtgt tgttttattc tccgcccctg aacgagggcg
     4381 ttcagtaagt cttttacctg aagcgtctgt gtttgtttta cgcaaaagta ccattcttcc
     4441 cagagtggct cagcttgcgc aaaaacttca tgatatggct caggaaggac aacgtatgcc
     4501 ttcctgtatc aatctgattg gaggcccaag ttctactgca gatatagaac tgatcaaagt
     4561 tgttggtgtg catggacctg tacatgcaac ttacttgatt attgatgact gctaatacat
     4621 tttagtatgt atatatgatt aacagctccg tttagctgag ctgttaatca ttcatttagt
     4681 tgtattagtc gtgacttgag cgtaagcgtc aatacggact ccgttaacgc cttacaatag
     4741 cagtcagtgt tcatgcctgt ttattgaggc aagggggagt ctgggcgcct gtaactaaag
     4801 atgccccccc cacgccttta aacttctcaa taaatgcgct aatcttctga aaaacggtct
     4861 gttttttcgt tttatattgc gggtttaacg gactaagttt tggtaatgtc tcgtttaatt
     4921 ctgtgccatt ttcggtggcg tattcgcgtt ttaacgacgt gcgaatatag cgcctggccg
     4981 cttcttcatt aagattttct tctttgatca atgcatctgc ttcacgttgt tgttcgcgtt
     5041 gagcaaaggt aaagaacgta tcaatgatgc tggctttgtc cggcaaatca tcaaggttcg
     5101 tttgctgaat aaaatcgacc accaggcctt ctttagcacg gttccccaga cttgaacgaa
     5161 ttaatcgttt gacctctacg gtcatttcgt ccttactttt gttttgtctg ctgtgttcga
     5221 aaatcaggcc aaggatataa tccaggttta tttcctgaga cttcagcaaa tcgacctcaa
     5281 aaaccacgtc atcccagtca gtggttgatt tttctttttt gtcagcttct ttttcgcggt
     5341 gctgccagtc acgaatatcg ttataggcag aacgataatc ctgaatcttg cgttcagcag
     5401 ggagacgaat catttgcaat tcagcgaact tttcatcatc cacataatgt tctgctttga
     5461 atttttctac cgcaacagga tcgctgagat cgatttgttg cagggctttc agcgtggcaa
     5521 attcatcata gttttgcagg acgttctcgg cacgcaggta ttcaccaaac agtttaacga
     5581 agtctttttt ctctttttcg ctttcaatac tggcagggtc agggaacctt tgttccagtt
     5641 ctgaaactac tgccataaag ccgcgtttag cttcgccagt ggcagcatct gtaaagcctt
     5701 ccatatactc tgcataactc ttttccagca ccacatttct ggtgttttta tcaccaaaaa
     5761 gtgtgatggc atcgatggtt gcctgctcca ggttacggaa tgtgacgatg ttaccgaagg
     5821 ttttcgtggc gtcataaatg cggttggtac gggaaaacgc ctgcatcaga ccgtgaaaac
     5881 gcaggttttt atcgacgaac agcgtgttca gtgttggagc atcgaagccg gttaaaaaca
     5941 tccccacgac gattaacagg tcaacatcct gatttttaac ccgctgagcc aggtcccgat
     6001 agtagttctg aaaatccttg ctatcagtac tgaaattcat ttgaaaatgg tcgttatatt
     6061 cacgaattgc agcatccaga aactctttag cactgctgtc cattgcgccg gtatcaaaag
     6121 tttcatcgga aatttcacca atggcatttt gttcttcatt ggcggcaaag gagaatattg
     6181 tcgcaatacg cagtggttta taatttgcag atttattagc ggcttcctcc tgtaagcgtt
     6241 taaacgtcgc gtaatagact ttcgcagcat ccacgctgct tactgccagc atggcattaa
     6301 aacctctgga acccgggaaa gtgcggtggg tcttctggcg gaagttattc aggatatatt
     6361 gcgtgatttc ctgaatacgc atgggatgaa gaaacgcctg ctgattttca gccgcactca
     6421 gttttttctc gtcagtttct gtctctaaag acttaaactg tggccgcaca tcgttgtagt
     6481 ccaccttgaa tttgagcact ttttcgtcac gaatcgcatc ggtaatcaca tacgaatgca
     6541 attcacgacc aaatacgctg gcggtcgttt ctgaacctga ggcgttttcc gggaaaatag
     6601 gtgtgccggt aaaaccaaac tgataatagc gtctgaattt cttcttcaga tttttctgcg
     6661 cttctccaaa ctggctgcgg tggcattcat caaatataaa caccacttgc tgattgtata
     6721 caggcaggtc gctttctgct ttcatcaggt tattaagttt ctgaatagtg gtgacgataa
     6781 ttttgttatc gtccttatcc agatttcgtt taaggcctgc ggtattttcc gagccgttaa
     6841 cactatctgg cgaaaaacgc tgatattcct tcatggtctg gtaatcgagg tctttcctgt
     6901 cgaccacaaa gaagacttta tcaataaagt ccagttctgt tgccagacgc gcggctttaa
     6961 agctggtcag tgttttaccg gaacccgtgg tgtgccagat atacccaccg ctttccggtt
     7021 ttgaccagtt cttcgctgta aaggagctct taattttcca cagaatgcgc tcggtggcag
     7081 caatctggta cggtcgcatc accagtagcg tctgactgct gtcaaaaacg ctgtagttca
     7141 ccagaacatt aagcagggta tgtttctgga aaaaggtggc ggtaaaatct ttgaggtctt
     7201 taatcagcgt gttgtctgat ttcgcccagt tcatggtgaa gtcaaaactg tttttatcgc
     7261 gctttgtcgt gttggcaaag taacgggtat cggtaccgtt ggaaatgacg aacagttgca
     7321 gatacttaaa cagggaattt tcgctgttaa aactctcctt gctgtaacga tgtaactggt
     7381 tgaaagcctc acgaatcgcc acgccacgct ttttcagttc gatttgcacc agcggcaaac
     7441 cattaaccag gatagtgacg tcataacggt tactgtgaga acctgcctgt tcaaactgcc
     7501 ggataatctg cactttgttg cgcatgagat tctttttatc tatcagatag atattctcaa
     7561 gacgcccgtt gtcaaaaata aagtcacaga tatagtcgat atggatttta cgggtcttat
     7621 ccagaatgcc atcgccaggg ttgtccagat actgctccgt gaaacgccgc cattcgctgt
     7681 cattaaacac cacgccattg aggctctgaa gctgttcccg gacattagcc agcatcgccg
     7741 actgtgattt tacggaaata aattcatagc cctgattctg caggtcctga atcagttcac
     7801 gttccaggtc cgattcactt tggtagctgt cgcctgtttg ctcagccttg atgtacttat
     7861 caagaacgat aaagttactg gtttcagcaa tggtgtgtgt ctgaagaatc atagcgcatc
     7921 ctttgtgccg tctggcgggg ccggaaggga gttaagggtg acttccggcg tgtaaaaatg
     7981 gtttatatac tgaccggatg ttaaggtggc ccggtcggta gcaattgtca attagttact
     8041 gacggtttca ggtttcggga aactgaacag taaatcacga tagtactcgt attgtttctg
     8101 gcgcaactcg atttcacgcg ggagaccttc ggtgatggag ttggtcaggg tgtcgaattt
     8161 atcgagtatt tcgacaatgc gagcttgttc ttctgaaggt ggtacaggta ttgggaattt
     8221 taaaaacatt ggtcgacgaa gtgaggtaac cgatgaactt actgatgctt tttgaatata
     8281 ggtaaa
//
LOCUS       DAZKGF010000194         8222 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZKGF010000194 DAZKGF010000000
VERSION     DAZKGF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.194"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..1225
                     /gene="lacY"
                     /locus_tag="ACHOQV_004352"
                     /pseudo
     CDS             1..1225
                     /gene="lacY"
                     /locus_tag="ACHOQV_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
     gene            1291..1902
                     /gene="lacA"
                     /locus_tag="ACHOQV_004353"
     CDS             1291..1902
                     /gene="lacA"
                     /locus_tag="ACHOQV_004353"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFP9632634.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVHFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKHYYYKDYKVESSV"
     gene            complement(2005..3186)
                     /gene="cynX"
                     /locus_tag="ACHOQV_004354"
     CDS             complement(2005..3186)
                     /gene="cynX"
                     /locus_tag="ACHOQV_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFP9632635.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQHRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQNAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVFWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(3192..3662)
                     /gene="cynS"
                     /locus_tag="ACHOQV_004355"
     CDS             complement(3192..3662)
                     /gene="cynS"
                     /locus_tag="ACHOQV_004355"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFP9632636.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(3693..4352)
                     /gene="cynT"
                     /locus_tag="ACHOQV_004356"
     CDS             complement(3693..4352)
                     /gene="cynT"
                     /locus_tag="ACHOQV_004356"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFP9632637.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTSIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            4462..5361
                     /gene="cynR"
                     /locus_tag="ACHOQV_004357"
     CDS             4462..5361
                     /gene="cynR"
                     /locus_tag="ACHOQV_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFP9632638.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            complement(5363..5454)
                     /locus_tag="ACHOQV_004358"
                     /pseudo
     CDS             complement(5363..5454)
                     /locus_tag="ACHOQV_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5494..6777)
                     /gene="codA"
                     /locus_tag="ACHOQV_004359"
     CDS             complement(5494..6777)
                     /gene="codA"
                     /locus_tag="ACHOQV_004359"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFP9632639.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLLDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(6767..8026)
                     /gene="codB"
                     /locus_tag="ACHOQV_004360"
     CDS             complement(6767..8026)
                     /gene="codB"
                     /locus_tag="ACHOQV_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFP9632640.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     IGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         2024 a         2193 c         2116 g         1889 t
ORIGIN      
        1 ttttggatgt tcggtttatt ctttttcttt tactttttta tcatgggggc ctacttcccg
       61 tttttcccga tttggctaca tgacatcaac catatcagca aaagtgatac aggtattatt
      121 tttgccgcta tttctctgtt ctcgctatta ttccaaccgc tgtttggtct gctttctgac
      181 aaactcgggc tgcgcaaata cctgcattat taccggcatg ttagtgatgt ttgcgccgtt
      241 ctttattttt atcttcgggc cactattaca atacaacatt ttagtaggat cgattgttgg
      301 tggtatttat cttggctttt gttttaacgc cggtgcgcca gcagtagagg catttatcga
      361 gaaagtcagc cgtcgcagta atttcgaatt tggtcgcgcg cggatgtttg gctgtgttgg
      421 ctgggcgctg tgtgcctcga ttgtcggcat catgttcacc atcaataatc agtttgtttt
      481 ctggctgggt tctggctgtg cactcatcct cgccgttttg ctctttttcg ccaaaacgga
      541 tgcgccctct tccgccacgg ttgccaatgc ggtaggtgcc aaccattcgg catttagcct
      601 taagctggcg ctggaactgt tcagacagcc aaaactgtgg tttttgtcac tgtatgttat
      661 cggcgtttcc tgcacctacg atgtttttga ccaacagttt gctaatttct ttacttcgtt
      721 ctttgccaca ggtgaacagg gtacgcgggt atttggctac gtaacgacaa tgggcgaatt
      781 acttaacgcc tcgattatgt tcttcgcgcc gctgatcatt aatcgcatcg gtgggaaaaa
      841 cgccctgctg ctggctggca ctattatgtc tgtacgtatt attggctcat cgttcgccac
      901 ctcagcgctg gaagtggtta ttctgaaaac gctgcatatg tttgaagtac cgttcctgct
      961 ggtgggctgc tttaaatata ttaccagcca gtttgaagtg cgtttttcag cgacgattta
     1021 tctggtctgt ttctgcttct ttaagcaact ggcgatgatt tttatgtctg tactggcggg
     1081 caatatgtat gaaagcatcg gtttccaggg cgcttatctg gtgctgggtc tggtggcgtt
     1141 gggcttcacc ttaatttccg tgttcacgct tagcggcccc ggtccgcttt ctctactgcg
     1201 tcgtcaggtg aatgaagtcg cttaagcaat taatgtcgga tgcggcgcga gcgccttatc
     1261 cgaccaacat atcaggacgg aatgatcgca atgaacatgc caatgaccga aagaataaaa
     1321 gcaggcaagc tatttaccga tatgtgcgaa ggcttaccgg aaaaaagact tcgtgggaaa
     1381 acgttaatgt atgagtttaa tcactcgcat ccatcagaag ttgaaaaaag agaaagcctg
     1441 attaaagaaa tgtttgccac ggtaggggaa aacgcctggg tagaaccacc cgtccatttc
     1501 tcttacggtt ccaacatcca tataggccgc aatttttatg caaatttcaa tttaaccatt
     1561 gtagacgact acacggtaac gatcggtgat aacgtattga ttgcacccaa tgttactctt
     1621 tccgttacgg gacaccctgt acaccatgaa ttgagaaaaa acggtgagat gtactctttt
     1681 ccgataacga ttggcaataa cgtctggatc ggaagtcatg tggtcattaa tccaggcgtc
     1741 accatcgggg ataattctgt tattggcgcg ggtagtgtcg tcataaaaga cattccacca
     1801 aacgtcgtgg ctgctggcgt tccttgccgg gttattcgcg aaataaacga ccgggataag
     1861 cactattatt acaaagatta taaagttgaa tcgtcagttt aaattataaa aattgcctga
     1921 tgcgctccgc ttatcaggcc tacaagttca gtgatctacg taggccgcat ccaacgtaaa
     1981 caaagcgcag gaacaagcgt cgcatcatgc ctctttgacc cacagctgcg gaaaacgtgc
     2041 tggtgcaaaa cgcagggtta tgatcatcaa cccaacgacg cacagcgcat gaaacgccca
     2101 gtccatcaag taattgccac tgatactacg cagcacacca gaaaaccacg gggcaagccc
     2161 ggcgatgata aaaccgatcc cctgcataaa cgccaccagt ttgccagcaa tagcgggttg
     2221 cgcagagtga tcgagtgcca gcagcaaaca gagcggaaat gcgccgccca gacctaaacc
     2281 acacaccatc gcccagaaga ccggcaattg cagcggcagc cagataaagc cgcagaaacc
     2341 caccagttgt aacgccagcg ccagcatcaa aagtttgcgc cgatcctgat ggcgagccat
     2401 agcaggcatc agcaatgccc ccgcagcttg cccaagcgtc atcaacgcca gcaaggaacc
     2461 gctgtactgc gcgctggcac caatctcaat atagaaagcg ggtaaccagg caatcaggct
     2521 ggcgtaacca ccgttaatca gaccgaagta aacacccagc gtccacgcgc ggggagtaaa
     2581 taccacgcga accggagtgg ttgttgtctt gtgagaagag gcgacctcgc gggcgttttg
     2641 ccaccaccag gcaaagagcg caacaacggc aggcagcgcc caccaggcga gtgtttgata
     2701 ccaggtttcg ctatgttgaa ctaaccaggg cgtaatggcg gcaccaagtc caccgccgcc
     2761 cattagagcc gcggaccaca gccccatcac cagtggcgta cggtgctgaa accgccgctt
     2821 aattaccgaa ggcatcactg cctgaatgat gccgatcccc actccaccaa gcagtgcgct
     2881 gctaagcagc agcgcacttt gggggtaaag ctcacgcatc aatgcaccga cggcaatcag
     2941 taacaaactg atggcgacgc tgcgacgttc gctgacatgc tgatgaagcc agcttccggc
     3001 cagcgccagc ccgcccatgg taaccaccgg cagagcggtc aacagggcag ccacgctaaa
     3061 gctcattccg cttgcctggc gcaattgcgg tagcagtggc ccgacggagg tgagcagtgg
     3121 tcgcatatta agaccaatca gcaccagaac cagcagcatc gtgaacgtct tttgatggtt
     3181 acgcatggct gtcagaacgg tttggtcggc agatatttac catctaaggt gatgaccgca
     3241 cgttcgccac cctccgggtc cgccactttc ttaacgtcga gtttgaagtt aatcgcgctg
     3301 ataatgccat cgccaaactt ctcatgaacc aacgctttca gggttgtgcc gtacacctgc
     3361 aacatttcat agaaacgata catcgttggg tcggttggaa tacggtcatc aatgcagcca
     3421 cgcagtggaa tcatctgcaa cagtagaatg gcgtcttcgt cgagatccag cttcgctccg
     3481 accaggcggg cggcgtcggc aggaagcgcc tgctgcccca gcaaagccgc ggttacaaag
     3541 gcttctgcca gaccggtgcc gtcggcaatc tcggcaaatg agagatcttt tttagctttg
     3601 ctgagcaaaa tggcatcggc aagatcaaga cgaatattgc ggttaatttg cgactgaatc
     3661 atggtggatc tcctgatggt ttaaaaataa ggttacgctg cggtcggttg gcgtagcggt
     3721 atggcacaaa cgcgaggatt agcggccagt ggcacaaact ggcgggttgc gccgtcaaaa
     3781 gctgcgatgc tgccgctttc aatgtcgtag acccagccgt gcagggcaat ccgcccctct
     3841 tcgagcgcca ggcgcaccga tggatgagtt tgcaaattag ccaactgagc aatgacgttt
     3901 tcacgtacca tcgccgctgc ttttgacggt aaatcgggat gcgggcgcgc ctcattaacg
     3961 acgcgggctg aatcggcata acgcagccag tgggagacgg caggcatatg gtccatgcac
     4021 tgacagctgg caatggaggt catcgcgcca cagttggaat gaccgcaaat cacaatgtca
     4081 gatacccgaa gtgcagcgac ggcatactcc accgaagcag aaacgccacc gggttccggc
     4141 ccgtaggaag ggacgatatt gcccgcgttg cgaataacga acagatcgcc aggctcacgt
     4201 tgcgtcacca gctcagggac cagacggctg tcggagcagg agataaaaag ggtgcgcggg
     4261 ctttgctgtg tcgccagctg tttaaacaag gcttcccgct tcggaaatgc ctcgcgctgg
     4321 aatttaagga atccatcaat aatctctttc accgttaacc tctgtctgtc tctggaatga
     4381 gaggcagact actcgccgat tgtcataagg taaaagactc atttatgatg agttctattg
     4441 gatttactta taggttggcg aatgctctct cgacatatca attactttct tgccgtggct
     4501 gaacatggca gcttcacccg tgcagccagt gcgttgcacg tctcccagcc tgcgctttcc
     4561 cagcagattc gccagttaga ggaaagtttg ggcgtgccgc tgtttgaccg tagcgggcga
     4621 acgattcgtc tcactgatgc aggagaagtc tggcgacagt acgccagccg ggcgttacag
     4681 gaactggggg cgggtaaacg ggcgattcat gatgttgccg atctgacgcg aggatcgctg
     4741 cgtatcgccg tcactcccac ctttacaagc tactttatcg gccctttaat ggcggatttc
     4801 tatgcgcgct accccggcat cacgctccag ctacaggaaa tgtcgcagga gaaaatcgaa
     4861 gatctgcttt gccgcgacga gttggacgtt gggattgcct tcgcgcctgt gcattcgccg
     4921 gagctggagg caattccttt actgacagaa agtttagcgt tagtcgtggc gcaacatcat
     4981 ccgctggctg cctgtgaaca ggtggcgttg agtcgcttgc atgatgaaaa actggtcctg
     5041 ctcagcgcgg aatttgccac cagagagcaa attgaccact actgcgagaa agcggggcta
     5101 catccacagg tggtcattga ggcgaactca attagcgcgg ttctggagct gattcgccgc
     5161 acttcccttt ccacattgtt gccagcagcg attgccacac aacatgacgg gcttaaagct
     5221 atatcccttg ccccgccact actggagaga acggcggttt tgttgcggcg gaaaaatagc
     5281 tggcagacag ccgccgcgaa ggcatttttg cacatggcgt tggataaatg cgcggttgtt
     5341 ggcggaaatg aatcgcggta accactgaca caattgcctg atgcgctacg cttatcaggc
     5401 ctacgagttc agtgctctac gtaggccgga taaggcgttc acgccgcatc cggcataaac
     5461 tacgctcgct ttgtcaacac acagcccagc cgatcaccgt ttgtaatcga tggcttctgg
     5521 ctgctccaga tatacggtgg tttgtgctgg ttgggtgctg gcaatcacct taccgccacg
     5581 taccgaataa cgtacaggaa cctgacggcg cagcgcgtca aacccatttt cagccggcag
     5641 gataatcagg ttggcgctgt ttccggcggc aatgccgtaa tcctgcaaat tcaacgtcct
     5701 ggcactgtgg tgggtgatta aattcaggcc atcgttaatc tgcccgtagc ccatcagctg
     5761 gcaaacatgc agccccatat gcagcacttg cagcatattc gccgttccca gcggatacca
     5821 cggatcgaag acatcatcgt gaccaaagca gacgttaatg ccggactcca gcatctcttt
     5881 aacgcgcgtg atgccgcgac gttttggata ggtatcgaaa cgtccttgca gatggatatt
     5941 gaccagcggg ttggcgacaa agttaatacc ggacattttc agcaagcgga acaggcgtga
     6001 ggtatacgcc ccgttatagg agtgcattgc cgtggtgtgg ctggcggtga ctcgcgcgcc
     6061 cattccttca cggtgcgcca gggcagcaac ggtttcgaca aagcgcgact gctcgtcatc
     6121 gatctcatca cagtgaacgt cgatgagacg gtcgtatttt tgcgccaggg cgaaggtttt
     6181 atgcagcgac tccacgccgt attcacgggt aaattcaaaa tgcggaatcg cccccactac
     6241 gtcagcccct aagcgtaacg cctcttccag caacgcttca ccgttgggat acgacaaaat
     6301 cccttcctgc gggaaggcga cgatttgcag atcaatccac ggcgcgactt cctgcttcac
     6361 ttccagcatt gctttcagcg cagtcagcgt tgcatccgaa acatcgacat gggtacgcac
     6421 atgctgaatg ccgttggcaa tctgccattt cagcgtttgc caggcgcgtt gtttcacatc
     6481 gtcatgggtt aataacgctt tgcgctcggc ccagcgttca atgccttcaa acagcgtgcc
     6541 ggactgattc cagttcggtt gtccggcggt ttgcgtggtg tccaggtgaa tatgcggctc
     6601 cacaaacggc ggtataacta aaccttgttc ggcatccagg ctgttttcag ttatgggcat
     6661 caccccggat tgcgcatcaa tggcgctgat ttttccgtcc agcagatgaa tctgccacag
     6721 cccctctttg ccgggtaacc gggcgttaat aattgtctgt aaagcgttat tcgacactgt
     6781 tagcctccac atgcgtcatt gcagccgtcg ttttgcgatt caaaatcggg ttaaggatca
     6841 gatagctcag cgcgccacct aataccgcgt tgaccggaac aatacccggt aaccagtggc
     6901 ctgcggcaat ccccaaggcg accgccagaa tcgccaccca attgacactc atcatacgcg
     6961 tggtcgcaaa gtgctcatag cggcgacggt tcatcagata gtcagcgatg atcacgcccc
     7021 ctaccggagg aatagctgcc gaaaggaagg tcagccagcc gacaaaattg ttatacagcc
     7081 ataatgcgca gaccgtaccg ataataccgt tgattaccga aagggtttta ctcgacatac
     7141 cagtaatgtt ggcgaaacct aaacccgacg catagagcgc gttatcgttg gtggtccaga
     7201 tattcagccc cagcaccaca atcgcaggca gcagcaggcc ctgagcaatc atcacatcag
     7261 agatatccgc catccccagt gccgcagcgc ccgctgcacc gaaaataaac atcaacgagt
     7321 tgccgaggaa aaaggccacc atcgccacca gcaccgccag tttggcatta cgaccaaacc
     7381 ggacaaagtc agcggtgagc gtgcccgcac tgataaatga ccccacaacc agcgccagcg
     7441 cgacattgaa atctaacggt tgtgcgggaa cgaccgcttt taatgcgtcc aggccgccca
     7501 tgccattaac ggccagccac acggaataac cgccaaggca ggcgatagcc ggaaccgcaa
     7561 tcaccgaaag aaccgtcagc gccgaaatgc caaagaagac ggtgacggtc atcagtaaac
     7621 cggaaacggc aatcagcaaa ttaatatcca gcccggttgc cttgcccacc ggaatggcaa
     7681 acatcgccac gccaacgcca aaccagccaa cctgggtgcc gcccagcagc agtgaaggca
     7741 gccatgagcc tttaacacca aaagagaagc gagcaagaag atgagtggtc aggccggttt
     7801 ttgcgccaat gtaaccaaga aatgaagtgt aaataccgag gagaagatta ccgatgagga
     7861 ctgcgaggaa gaaatcatga tagctaagac cgattccgag agtgccgccg gtccacatac
     7921 tggcggaaaa gaaggttaat cccagcatga cgaacgtcaa tgccaatacc cctttccgcg
     7981 ccgactgcgg gactggcccc tggctaaagt tgttatcttg cgacacgaaa ttcccccttt
     8041 ttgctgtttg aaacccaaaa aatccgccgc attctattca tctgaagata aaaagcaatc
     8101 gttttcgtag gggaatatat ttcttatatg aatgagtaca aaaaggcggg ggaaacttat
     8161 ctgtgtaaaa acagtgggta ggcatgataa gacgcgtcag cgtcgcatca ggcatctgcg
     8221 ca
//
LOCUS       DAZKGF010000195         8051 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZKGF010000195 DAZKGF010000000
VERSION     DAZKGF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.195"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..810
                     /locus_tag="ACHOQV_004361"
     CDS             <1..810
                     /locus_tag="ACHOQV_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFP9632641.1"
                     /translation="LENAAERAQALKADIDSQIQMFEQRLKLLNEAVLFSAPEGMALT
                     SGEDMQMSAARNVAVNAGGDISTGTMGNLTALAGEKLGLFARTGQLSLKSGEGAVDVQ
                     AQNGSMRLFAEKKLTISSESDISFTGKKRITLIGGGSYLRLEAGKVEYGTMATCMRRV
                     KRTMTEAAQSMPMTFPRMSSSHIYSAIYRLLDKHGNILANTPCRLTTPSGQTVMGYSD
                     NEGRSVPVYTRQQEDVELHFFVKKPQPEETMWFVGETDTQQLETELRESQS"
     gene            810..2093
                     /locus_tag="ACHOQV_004362"
     CDS             810..2093
                     /locus_tag="ACHOQV_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFP9632642.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGRNRTRMTILEIHDCRPEE
                     HQWVDQVVNVTMKAWQISGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKVVSDL
                     SEGAVRGVAEGWNALSKETQLVLNSAASWLNDSGEWVYRQGSEVWVWASQTGKSVKSW
                     TNTQIKAAWTEIQHVTDMTLDMLKQVDWVQVLTNVAKTVGVILVAVGVGGLVVTLGIP
                     EAIIGAFLLIVRLAISAWGTLATILGTGTAVLAVS"
     gene            2106..3320
                     /locus_tag="ACHOQV_004363"
     CDS             2106..3320
                     /locus_tag="ACHOQV_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3396 domain-containing protein"
                     /protein_id="HFP9632643.1"
                     /translation="MELHDFELNYIEKWLPEASVKYKDGSPAVNLGLIITVFFKDGHT
                     PEVRRRMVECVDRFYGEFKPWLKKTITKNWVGITDNNYAKKRQEILDSTPEEIFSWYL
                     SSAEKDFLAPDYSILIMGKRIFHNDNNRSVIKLTLPLSLLKETDGKQRYEAWLMWLCD
                     TFSVESGYAGLSFALPYARERMFPCEFALAQRFSGVMVDSLGTLEGGEAVEGLKGACW
                     YTILGTPWLEKLGGAERVKHRLAKTPEISLLPYNNGVVLKAGELPPPLGEMKTEGLSP
                     LLVKVNQVIRSIRYDGRNGLHFYSEYEGLQFEEASSMAWFARFDEASALLDKEEMGKS
                     CAPERIKCWTDEIAPHAGQWAAMVNGTTEYIQTREGQKMPPFEDKHGKQHRACWKLLK
                     RDDNGSVYTLPE"
     gene            3334..3687
                     /locus_tag="ACHOQV_004364"
     CDS             3334..3687
                     /locus_tag="ACHOQV_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFP9632644.1"
                     /translation="MTVQAGERLGLFARTGQLSLKSGEGAVDVQAQNGSMRLFAEKKL
                     TLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTMATYLRRTKRTMVAAPATMPLP
                     IPLLLDLYPPLNGQI"
     gene            4016..4471
                     /gene="tssE"
                     /locus_tag="ACHOQV_004365"
     CDS             4016..4471
                     /gene="tssE"
                     /locus_tag="ACHOQV_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP9632645.1"
                     /translation="MSHHSPSLYETLYGNFTGGLDLHQVCEENQVIVSVLDNMQRILN
                     TRAGSLKHLPDYGLPDMTTILQGMPGTAHQLMRVLSDVLLKYEPRIKRVDVTMQEQTQ
                     PGELHYVIDAELKDAGLVRYGTTFMPEGRVLLRHLKQQQFVDNSSYYHV"
     gene            4539..4844
                     /locus_tag="ACHOQV_004366"
     CDS             4539..4844
                     /locus_tag="ACHOQV_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632646.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRVFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            4899..5213
                     /locus_tag="ACHOQV_004367"
                     /pseudo
     CDS             4899..5213
                     /locus_tag="ACHOQV_004367"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            5538..5687
                     /locus_tag="ACHOQV_004368"
                     /pseudo
     CDS             5538..5687
                     /locus_tag="ACHOQV_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5862..6263
                     /locus_tag="ACHOQV_004369"
                     /pseudo
     CDS             5862..6263
                     /locus_tag="ACHOQV_004369"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            6245..6469
                     /locus_tag="ACHOQV_004370"
                     /pseudo
     CDS             6245..6469
                     /locus_tag="ACHOQV_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            6472..6758
                     /locus_tag="ACHOQV_004371"
                     /pseudo
     CDS             6472..6758
                     /locus_tag="ACHOQV_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            6780..>8051
                     /locus_tag="ACHOQV_004372"
     CDS             6780..>8051
                     /locus_tag="ACHOQV_004372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632647.1"
                     /translation="MKRISVNVLDLYLDSKNPRHDPINNRMEIINYLIQNEKIKVLAK
                     SIAENGTSPLETLAVMENENGHFIALEGNRRVCALTLLNDPDLCPENERAYFRKLSDN
                     SDVIPDAVECVLFENREDANKWIELRHNGEQDGAGLLTWNATQKARHFGSSDNTLALK
                     LLDYAETKGFISKQKRDGILTTASRFLGNPVFRSTMGIRTGRSESNVMIDVSRDEFDK
                     VLERFINDLINGENNVTSRSKKEDWENYAKQLRSEGIAPKGHCEPFALFSTEHSNASG
                     KGSTISKIVKNNQSPNNRKYIIPSDFKVNIPDPILKRVFDELRTLECSEYTLASSVVC
                     RAFLENIYSLAYEKFYGNYPEGMAEHVVMEKIINKIEEDKNSLTKKEKKALEALKRVK
                     TDTTNPLSPKTLGANAHAGFYPDPTALKVHWD"
BASE COUNT         2203 a         1779 c         2140 g         1929 t
ORIGIN      
        1 ctggaaaatg ccgcagaacg ggcacaggcg ctgaaggctg atattgacag tcagatacag
       61 atgtttgagc agcggctgaa actgctcaat gaagcagtgc ttttctcggc accggaagga
      121 atggcgctga ccagcggtga agatatgcag atgagtgcag cacgaaatgt ggctgtcaac
      181 gccggggggg atatcagtac cggaacaatg ggcaacctga ccgcgctggc cggagagaag
      241 ctggggcttt ttgcccggac cggtcaactt agcctgaaat ccggggaagg ggcagtggat
      301 gttcaggcgc aaaacggcag catgcggttg tttgctgaga agaagctgac catttcgtca
      361 gaaagtgata tctcgtttac cggcaagaaa cgcatcacgc tgattggcgg ggggagttac
      421 ctgcggcttg aagccgggaa ggtcgagtat ggtacgatgg caacctgtat gcgtcgggtg
      481 aagcggacga tgacggaggc tgcgcagagt atgccgatga cattcccccg tatgagttct
      541 tcgcatatct actcggctat ttatcggttg ctggacaaac acggcaatat tctggccaat
      601 accccctgtc gcctcaccac tccatctggt cagacagtaa tggggtatag cgataacgaa
      661 ggccgctccg tgccggttta cacccgccag caggaggatg tggaactgca tttttttgtt
      721 aaaaaaccac aacccgaaga aacgatgtgg tttgtggggg aaaccgatac gcagcaactg
      781 gaaaccgaac tcagggagag ccagtcataa tggaaatgcc cgatttaccc gagacaccga
      841 catctgatat tcccactggg gcgaacaacc agccgtggga ctgcaccacg acgggtgtcg
      901 aaatggtcga aaagcagtcg cgtctgccaa ccacggaaaa ccgtcagtac ctgcatataa
      961 aggtggaata tgccatgcgt tttccgcaac tgtcggcaca aatccgccgg aaaggtaaaa
     1021 cttaccagat gagccttaag cagaccgtta tgagtggtct tattcgcgtg gatgaaatcg
     1081 cccgcaatta ctgctggtat tataaggcgg aggtggcgtt tgatatgcgc accacgccgc
     1141 cacgcccttt tctgagcagt acgctgctgg aaaaagacgg tgctggccgt cgccacacga
     1201 ccaatccttt ccctgtgccc ggcaggggca tttaccgccg cccggacgtg attattgtta
     1261 aaaataagga agaccgctgg cccggactgg cggggccgga taccgaaggc aatatgcaca
     1321 gcgataacct ggcccgcctg gtggaggtga agtttccggg agataggctg tatgaagacc
     1381 aatatgaaga gtatatgcag attgtgggca ggaaccgaac ccgcatgacc attctggaga
     1441 ttcacgactg tcgcccggaa gagcaccagt gggttgacca ggtggtcaac gtgacgatga
     1501 aagcctggca gatcagcggc gcgaaatact ggccgctggc tctttacccg ccagtcttta
     1561 tgccccgtcc gccatcaacc cccgttgctg ccagaataga gccctggact tacgctggta
     1621 aagtagtatc tgacctcagt gagggggcgg tgcgtggtgt ggctgaaggc tggaatgccc
     1681 tgtcaaaaga gacgcaactg gtcctgaatt cagcagccag ctggcttaac gacagcggtg
     1741 agtgggttta ccggcaggga agtgaggtct gggtctgggc cagccaaacc ggtaagtcgg
     1801 tgaaatcctg gaccaacacc cagattaagg ccgcatggac agaaatacag cacgtcaccg
     1861 atatgacgct tgatatgctg aaacaggtgg actgggtaca ggttttaacc aacgtcgcga
     1921 aaacggtcgg cgttattctg gttgctgtgg gtgtcggcgg actggtcgta acgctgggta
     1981 tcccggaagc cattattggc gcgtttttac tgattgttcg tctggcaata agcgcctggg
     2041 gaacactggc gacgattctc ggtacgggta cggcggtact ggcagttagt taaacaggag
     2101 caggtatgga attgcatgat tttgagctga actacatcga gaaatggctg ccggaagcct
     2161 ccgtgaaata caaagacggc agtccggcag tcaatctcgg tctgattatc accgtctttt
     2221 ttaaggacgg acatacgcca gaagtccgcc gcaggatggt ggagtgcgtg gatcgcttct
     2281 atggggagtt taaaccctgg ctgaaaaaaa cgatcaccaa aaactgggtt ggcatcactg
     2341 ataataacta cgccaaaaag cggcaggaga tcctcgattc aacgcctgaa gagatttttt
     2401 cctggtatct gagtagcgca gagaaggatt ttttagcccc cgattacagt attttaatta
     2461 tggggaaaag aatttttcat aatgacaata atcgttcagt cattaagtta acactcccgt
     2521 tgtcactgct gaaagaaact gatggaaagc agcgttatga agcctggctg atgtggttgt
     2581 gtgacacctt ttcggtggaa agtggttatg cagggttgtc gtttgcactt ccttatgccc
     2641 gtgagcgaat gttcccctgt gaattcgccc tggcgcagcg tttttccggt gtgatggtgg
     2701 attctcttgg gacgcttgaa ggcggtgaag ccgttgaggg gctgaaaggt gcctgctggt
     2761 acaccattct cggtacgccg tggctggaaa aactgggggg ggcagagaga gtaaaacatc
     2821 gtttagctaa aacgcctgaa attagcctgc taccctataa caatggtgtc gtacttaaag
     2881 ccggtgaact ccccccgcca ctgggagaga tgaaaacaga aggtttatcg ccgctgctgg
     2941 tgaaggtgaa ccaggtcatt cgttccattc gctatgatgg acgcaatggt ctgcattttt
     3001 actcagaata tgaaggactt cagtttgaag aagcgtcttc aatggcatgg tttgcccgtt
     3061 ttgacgaagc cagtgccttg ctcgataaag aagaaatggg gaaatcttgc gcccccgaac
     3121 gcattaaatg ctggaccgat gaaatagcgc cccatgccgg acaatgggcc gcgatggtta
     3181 atggcaccac cgagtacatc cagacccgcg aagggcagaa aatgcccccg tttgaggata
     3241 agcacggtaa gcagcacagg gcttgctgga aactactgaa acgagatgac aatggtagcg
     3301 tttatacact acctgagtaa ggtcgtgtgc tgaatgaccg tgcaggccgg agagaggctg
     3361 gggctttttg cccgcaccgg tcaacttagc ctgaaatccg gggaaggggc agtggatgtt
     3421 caggcgcaaa acggcagcat gcggttgttt gctgagaaga agctgacgct gagctcagcg
     3481 agtgacatct cctttgccgg gaaaaaacgc atcacgctga ttggcggggg aagttaccta
     3541 cggctggagg cggggaaaat agagtacggt acgatggcga cgtatctgcg gcggacgaaa
     3601 cggacgatgg tagcagcccc ggcaacaatg ccgctcccca taccgcttct cctggacctg
     3661 tatccaccac ttaatggcca aatttgaatt gaatgtctcc tcaatgccat caaagtcaac
     3721 gatgctatcg ttcagggagc gtagtcaatg gatgtcacaa aaatgatgca ggtgctacag
     3781 gatttgtttt tatgctgcta aatgtacagc gacttcgtga tatggggttg ttggtgtttt
     3841 ttataataat gctttttacg gaatcaaaaa aacagacaac atattaatta tttgagaaag
     3901 cagcgtcaaa aaggaaaagt taaaatgtcg aatcgatttt ttactccggt aatcttcttt
     3961 ccagtgtgtt gattgaacta tttccaagat taaatgatta tgagaagaag tcgtgatgtc
     4021 tcatcatagc ccctcccttt acgaaacctt atacggaaat tttaccggcg gcctcgacct
     4081 ccatcaggtc tgtgaagaaa atcaggtcat cgtgtcggtg ctcgataaca tgcagcgtat
     4141 tctgaatacc cgggccggaa gccttaaaca tcttccggac tatgggctgc cggatatgac
     4201 aaccattctg cagggaatgc cgggtacggc gcatcaactg atgcgcgtgc tttcagatgt
     4261 tctgctgaag tatgagccac gtattaagcg tgtcgacgtg acgatgcagg aacagactca
     4321 gcctggtgag ttgcattatg tcattgatgc agaacttaaa gatgccgggc tggttcgtta
     4381 cggaacaaca tttatgccgg agggcagagt gttgctgcgc catcttaagc aacagcagtt
     4441 tgtggacaat tcatcttatt atcatgtctg atttttttat ccgggctgcc cctgtgtggg
     4501 tggtccggat gaaaaatata ttttttgtga ttttgattat gaacccgtta tttaacgata
     4561 ttcagatgcg tcttttttat ctgaaccact ctccgtactc ctggcactgg aatgtcagat
     4621 tcagacctca ggaagccgta tatattggca gcgatacctg tcatatcacc attacctgta
     4681 atcagtccgg ttttcattta acccgggacg gacaacgggt attcactgag cgttatatca
     4741 gaaaccttaa tgagctgtta ccagttctga aacggcggtg ggatgtgaca ccggcaatca
     4801 tccgtgcagt ggaatacctt tcccgggtcc cggtatcaca ctgacattct ttccgtaact
     4861 gttaatcatg atttaataca gcaacactga atctgccgat gacttctgaa gtgaaactga
     4921 aaacaggtgg tgacccccgg tcattcccgg attatgctgc cctgcgcgat gaaatcagca
     4981 aactgaccca cccggcacgc ccggatgtgg actggcgtta tgtggaaacg ctctgcctgc
     5041 gcctgtatga acacaatggc gtggaattac agacagcatc ctggtacacc attgcccgga
     5101 tgcatacaac agggttaagt ggcctgaatg aagggctggc tctgattgtg gcgctgaccc
     5161 ggcatcactg gtcagtgatg tggccgctga atacccacgc ccggctggaa attattaccg
     5221 gtctgttcaa ccgtctgcaa aaaacgctcc gggccatgcc accggacgac cgtgacaacc
     5281 tgcccctgct gtatcagacc gaaacattcc tgaaggcact gagtgataca ctggcccggc
     5341 acgaacttaa acagagcagt aaagtggctt taccggaaac catggtgaag ggatatatca
     5401 cccgtctgga aaatcagccg atgcagggcg aagtgtcgtc tccggtaacc cttcctgctc
     5461 gggcactccg gtctgatgct ccggatgtac agggacatca gcccctgccg cactcccgtc
     5521 tggtgtatat tgtttctgaa acaaaagcag agcccacacc ctcccttcaa aaatccccgc
     5581 ccacgtttct gaaaccgttt gtggctggtg tctgtgcggc attgctggcg gtcagcgtgg
     5641 ccattccggg atggcagttt ctgacacagc catcaccggc ggagcagcaa ctcagggcgc
     5701 tgctggcacc actaccggat atgctttcag cagaccagat ggcacagctc agcagaacac
     5761 cctcactgtt aaatcatgcg tcagactgga taacactttc aggtcagcag ataacgcgac
     5821 tgacagaact accgctgaca tacaacctgc agcgtagtgc gcagctgttg cagcaactga
     5881 tggtactttt ccccgataat cctcgtgtac aggaaatggt ggataactgg cagaagagcg
     5941 taaggagtcg tgcgctcccg gaagaggcga tgacgggctg gaatgaaggc atgacccgct
     6001 tacagcagct ggctgaacgc ctgaaccgcc tggatgaaca gcggggaaaa tacatgacgg
     6061 tcagtgaact gaaaacggag gtgtttggca tcatgcaggc ttttaaccgg cacataccgg
     6121 cagaagagca gttacgtcgc tatgatgaag tccgtaatca gaatggcagc gaatctcagc
     6181 agaaactggc gcaggatgct ctgatggaac agttgaaccg ctactggctt ctgagacatg
     6241 gagatgctgg gaatccggcg tgacttgggt tcgccaacca ccggaagacg ccattacgga
     6301 tatggcgtat tttccctttt accggtatgc catggccttt gaaggattca ttccggcatt
     6361 ttcatcttgg tttgcaggta accggtgtgg agtggcaata ctgaccggtg tccgggcgga
     6421 tgaatccctc aactgcttta tggggctggt gtctcagcgc aaactgagag aattacgtgg
     6481 gtaggatcag atgatggtat tggaactgaa ggtgatatgg gaaaatggtt gttattattt
     6541 tgtctgctgt agctcagtct gaatgacaac atatacttga aagaacaaat gaaggccgtc
     6601 tcgaagccga agtaaagggt atttaatttg ggcgaaaaag aacggtcaac agggaacaag
     6661 tacaaacctt acgaagtctg ggtatcggcg ctacagaaat ttcgcgaaag atgaaattta
     6721 cacgttctac tgtgtataaa atattagagg gtaaataata ttttcatatg gagggttaca
     6781 tgaaacgtat ttcagtaaat gttttagatt tatatttaga tagcaaaaat cctcgacacg
     6841 atcctattaa taaccgcatg gagataatta actatctgat tcaaaatgaa aaaattaaag
     6901 tattggctaa aagtattgct gagaatggaa caagtccatt agaaacactt gcggtgatgg
     6961 agaacgaaaa tggacacttt attgcccttg aggggaatag gagagtttgt gcattaactc
     7021 tgcttaatga tcctgattta tgtccagaaa atgagagggc ttatttcaga aaactatctg
     7081 ataatagtga cgtcattcct gatgcagttg aatgcgtttt atttgaaaat agagaggatg
     7141 caaataaatg gattgaattg cgccataatg gagaacaaga cggtgcgggg cttcttactt
     7201 ggaatgcaac tcaaaaagca agacatttcg gttcttctga taatacatta gcgttaaaat
     7261 tattagatta tgctgaaact aaaggattta tttcgaagca aaaacgagat ggcattttga
     7321 caacagcatc gagattctta ggtaatcctg tattcaggtc aactatggga attagaacag
     7381 ggcgttcaga atctaatgtg atgattgatg tttccagaga tgaatttgat aaagttcttg
     7441 aaaggttcat caatgactta attaatggtg aaaataatgt cacatcaaga tcaaagaaag
     7501 aagactggga gaattatgca aaacagctaa gatcggaagg tattgccccc aaaggacact
     7561 gtgaaccatt tgctcttttt agtacggaac atagcaatgc ttctggtaag ggaagtacta
     7621 taagtaaaat agttaaaaat aaccagagcc ccaataatag aaaatatatt attccttctg
     7681 attttaaagt taatatacct gatcctatat taaaaagagt atttgatgag ctcagaacac
     7741 ttgaatgttc agaatacaca ttggcttctt cagttgtatg tagggctttt ctggaaaaca
     7801 tctattcatt agcatacgaa aaattttatg gaaactatcc tgaagggatg gctgaacatg
     7861 tagtaatgga gaaaataata aataagatag aagaagacaa gaatagcctc actaaaaaag
     7921 aaaagaaagc tctggaggct ctgaaaaggg ttaaaactga tacgaccaat ccactatctc
     7981 caaaaacgct aggagcaaat gcccatgcag gcttctaccc agatccaacg gcgttaaaag
     8041 tccactggga t
//
LOCUS       DAZKGF010000196         7859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZKGF010000196 DAZKGF010000000
VERSION     DAZKGF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.196"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            127..216
                     /gene="kdpF"
                     /locus_tag="ACHOQV_004373"
     CDS             127..216
                     /gene="kdpF"
                     /locus_tag="ACHOQV_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFP9632648.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            216..1889
                     /gene="kdpA"
                     /locus_tag="ACHOQV_004374"
     CDS             216..1889
                     /gene="kdpA"
                     /locus_tag="ACHOQV_004374"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFP9632649.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFIICTGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLALMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1912..3960
                     /gene="kdpB"
                     /locus_tag="ACHOQV_004375"
     CDS             1912..3960
                     /gene="kdpB"
                     /locus_tag="ACHOQV_004375"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFP9632650.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTFISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPVDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3969..4541
                     /gene="kdpC"
                     /locus_tag="ACHOQV_004376"
     CDS             3969..4541
                     /gene="kdpC"
                     /locus_tag="ACHOQV_004376"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFP9632651.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASANVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4534..7218
                     /gene="kdpD"
                     /locus_tag="ACHOQV_004377"
     CDS             4534..7218
                     /gene="kdpD"
                     /locus_tag="ACHOQV_004377"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFP9632652.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     LELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGEKLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7215..>7859
                     /gene="kdpE"
                     /locus_tag="ACHOQV_004378"
     CDS             7215..>7859
                     /gene="kdpE"
                     /locus_tag="ACHOQV_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFP9632653.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFIT"
BASE COUNT         1585 a         2066 c         2357 g         1851 t
ORIGIN      
        1 attagttaaa tcatggcttt tgccattttt atactttttt tacaccccgc ccgcagattt
       61 ttgcgaaatc tttgcagcca gaattctact cttccggtat cacttttagg ccactggagg
      121 tgcactgtga gtgcaggcgt gatagccggc gtgttgctgg tgtttttatt actgggttat
      181 ctggtttatg ccctgatcaa tgcggaggcg ttctgatggc tgcgcaaggg ttcttactga
      241 ttgccacgtt tttactggtg ttaatggtgc tggcgcgtcc tttaggcagc gggctggcgc
      301 ggctgattaa tgacattcct cttcccggta cgacgggcgt tgagcgcgta ctttttcgcg
      361 cgcttggcgt ctctgaccgc gagatgaact ggaagcagta tctctcagcc attcttggcc
      421 tgaatatact ggggctggcg gtgctgtttt ttatgttgct cggtcagcac tatctgccgc
      481 ttaatccaca gcagttgcca ggactgtcgt gggatctggc gctgaatacc gccgtcagct
      541 ttgtcacaaa taccaactgg caatcttaca gcggtgaaac cacgttgagc tatttcagcc
      601 agatggcggg cttaacggtg caaaactttc tttctgccgc cagcgggatt gcggtgattt
      661 ttgccctcat acgtgcattt acccgccaga gcatgagcac actcgggaat gcctgggtcg
      721 atctgctacg catcacgtta tgggtgctag tccctgtggc gttgttgatc gcattgtttt
      781 ttattcaaca aggtgcgctg caaaactttc tgccctatca ggcggtgaat accgttgaag
      841 gagcgcaaca gctgttaccc atggggcctg tagcttctca ggaagcgatc aagatgctcg
      901 gcactaacgg cggtggcttc tttaatgcca actcgtcgca tccgtttgaa aacccaaccg
      961 cactgaccaa cttcgtgcag atgctggcga tcttcttgat cccaacagcg ctgtgctttg
     1021 cctttggcga agtggcgggc gatcgccgtc aggggcgcat gttgctgtgg gcgatgtcag
     1081 tgatttttat catctgcaca ggcgtggtga tgtgggcaga agttcagggt aatccgcatc
     1141 tgctggcact gggcgcggac agcagcatca atatggaagg taaagagagt cgtttcggcg
     1201 tgctggtcag tagcctgttt gcggtcgtga ctacggcggc ttcctgtggc gcggtgattg
     1261 cgatgcatga ttcgtttacc gctcttggtg gcatggtgcc gatgtggctg atgcaaattg
     1321 gtgaagtggt gttcggcggt gtcggttctg gtctttacgg catgatgctg tttgtcctgc
     1381 tggcggtgtt tattgctggg ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa
     1441 tcgacgtacg cgagatgaaa ctgaccgcgc tggcgattct ggtcacccca acgctggcgc
     1501 tgatgggcgc ggcgctggca atgatgaccg acgccggacg tagcgccatg ctcaaccctg
     1561 gcccgcatgg ttttagcgaa gtgctgtacg ccgtgtcatc cgccgctaac aacaacggca
     1621 gcgcctttgc cggattaagc gccaactctc cattctggaa ctgtttgctg gcgttctgca
     1681 tgtttgtcgg tcgcttcggg gtaattatcc cggtgatggc aattgccggt tcgctggtga
     1741 gtaaaaagag ccagcccgcc agctccggca cgctgccaac gcacggcccg ctgtttgttg
     1801 gcctgttaat cggcaccgtg ttgctggttg gcgcactgac ctttatccct gccctggcgc
     1861 ttggtccggt ggcggaatat ctctcctgat gatattgagt gagcactgaa tatgagtcgt
     1921 aaacaactgg cgctattcga accaacactt gtcgttcagg cgctgaaaga agcggtgaaa
     1981 aaattaaacc cgcaggcgca atggcgtaat ccagtgatgt ttatcgtctg gatcggcagt
     2041 ctgttaacca cctttattag cattgcaatg gcaagcggcg cgatgcctgg caatgcgctg
     2101 tttagcgcgg caattagcgg ctggctgtgg gtcaccgtac tgttcgctaa tttcgccgag
     2161 gcgctggcag aaggccgcag taaagcgcaa gccaatagtc tgaaaggggt gaaaaaaact
     2221 gcctttgccc gcaagctgcg tgagccgaaa tatggcgctg cggcggacaa agttcccgca
     2281 gatcaactgc gtaaaggcga tatcgtactg gtagaagctg gcgatattat cccctgcgat
     2341 ggtgaagtca ttgaaggcgg cgcatcggtc gatgaaagcg ccatcaccgg ggaatcggct
     2401 ccggtgatcc gtgaatccgg cggcgatttt gcctccgtca ctggcggcac gcgtattctt
     2461 tctgactggc tggtgattga gtgcagcgtt aaccccggcg agacatttct ggatcggatg
     2521 atcgcgatgg tggaaggcgc gcagcgacgc aaaacgccga acgagattgc cctgaccatc
     2581 ctgctgattg ccctgactat cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc
     2641 gcatggggtg gtaatgcagt cagcgtaacg gtactggtgg cgctgttggt ctgtctgatc
     2701 ccaaccacta ttggcggcct gttgtcagcg atcggcgtcg ccgggatgag ccggatgttg
     2761 ggcgcgaatg tgattgccac cagcgggcgt gcagttgaag cggcgggcga cgtggacgtc
     2821 ctgctgctgg ataaaactgg caccatcacc ctcggtaacc gtcaggcatc agagtttatc
     2881 cctgcgcagg gcgtagagga aaaaacgctg gccgacgcag cacaactggc ttcgctggcc
     2941 gacgaaacgc cggaaggccg cagtattgtg atcctcgcca agcagcgctt taacctgcgc
     3001 gagcgcgatg tgcagtcgct tcacgccact tttgtaccgt ttactgcgca aagtcgaatg
     3061 agcgggatca atatcgacaa ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgccgc
     3121 catgttgagg ctaacggtgg tcacttccct gtcgatgttg atcaaaaagt cgatcaggtt
     3181 gcgcgtcagg gggccacgcc gctggtggtg gtggaaggtt ctcgtgtgct gggcgttatt
     3241 gcgctgaaag atatcgtcaa aggcggtatt aaagagcgct tcgcccagct gcgcaaaatg
     3301 ggcattaaaa cggtgatgat taccggcgat aaccgtctga ctgccgccgc cattgctgcg
     3361 gaagcgggtg tcgatgattt tctcgccgaa gcgacaccgg aggccaagct ggcgttaatt
     3421 cgtcagtatc aggcggaagg ccgtttggtg gcgatgaccg gcgacggcac caatgatgct
     3481 ccggcgctgg cgcaggcaga tgtcgcggta gcgatgaact ccggtactca ggcggcgaaa
     3541 gaagcgggca atatggtgga tctcgactct aacccgacca agttgatcga ggtggtgcat
     3601 attggcaaac agatgctgat gacccgtggc tcgctgacca ccttcagcat tgccaacgat
     3661 gtggcgaaat acttcgccat tattccggcg gcgttcgcgg caacgtatcc gcagttaaat
     3721 gcgctgaaca tcatgcgcct gcattcgccc gactccgcca ttctcagtgc ggtcattttc
     3781 aacgccttga ttatcgtctt tttgattccc ctggcgttaa aaggcgtgag ttataaaccg
     3841 cttaccgctt ctgccatgtt gcgccgtaac ttatggattt acggtctggg tgggctgctg
     3901 gtgccgttta ttggtatcaa agtcattgat ttactgctga ccgtttgcgg tctggtgtga
     3961 ggtttaccat gcgtggatta cgtccggcat tatcaacatt tctctttttg ttactgatta
     4021 ctggcggtgt ttacccgctg ctgaccaccg cgctggggca atggtggttt ccctggcagg
     4081 ccaacggttc gttgattcgc gaaggtgata cggtgcgcgg ctcggcattg atcgggcaga
     4141 attttactgg caacggctat tttcatggtc gcccgtcggc aacggcagaa atgccctata
     4201 atccacaggc ttctggcggg agcaatctgg cggttagtaa cccggagctg gataaacaaa
     4261 tcgctgcacg cgttgccgca ttacgggccg ctaacccgga tgccagcgcg aacgttccgg
     4321 ttgaactggt gacggcgtcg gcaagcgggc tggacaataa catcaccccg caagcggcgg
     4381 cctggcagat cccacgcgtg gcgaaagcgc gtaatctcag cgttgaacag ctcacgcagc
     4441 tgatcgcaaa atatagccaa caaccgctgg tgaaatatat cggccagccg gtcgtcaaca
     4501 ttgtcgaact caatctggcg ctggataagc ttgatgaata acgaaccctt acgtcccgac
     4561 cccgatcgtc tgctggaaca aactgccgcg ccgcatcggg ggaagctgaa agttttcttc
     4621 ggtgcctgcg caggcgtcgg caagacctgg gcaatgctgg cagaagccca gcgactgcgg
     4681 gcgcaagggc tggatattgt ggttggcgtg gtagaaaccc acgggcggaa agataccgcc
     4741 gcaatgctgg aagggctggc tgttctgccg ttaaaacgcc aggcgtaccg tgggcggcac
     4801 atcagcgagt ttgatctcga tgccgccctc gcccgccgtc cggcactgat cttaatggac
     4861 gaactggcgc acagtaatgc gccaggttct cgtcatccca aacgctggca ggacatcgaa
     4921 gagctgctgg aagctggcat tgatgttttc actaccgtca acgttcaaca tctggaaagc
     4981 ctgaatgatg tggtcagcgg cgtcaccgga attcaggttc gggaaaccgt gcccgatcct
     5041 ttttttgatg ccgccgacga cgtagtgctg gtggacttgc ccccggacga tctgcgccag
     5101 cgactgaaag aaggcaaagt ctatattgcc gggcaggcgg agcgcgccat tgaacatttt
     5161 ttccgcaaag gcaatctgat cgccctgctc gaactggcac tgcgccgtac tgccgatcgc
     5221 gtagatgagc aaatgcgcgc ctggcggggg catcctggcg aagagaaagt ctggcacacg
     5281 cgcgacgcga tccttttatg catcggccat aacactggta gtgagaaact ggtccgcgcg
     5341 gcggcgcggc tggcgtcacg gctgggtagc gtctggcacg cggtgtatgt tgaaacccct
     5401 gccctgcatc gcttaccgga gaaaaaacgt cgggcaattc tcagcgcctt acgtctggcg
     5461 caggagctgg gcgcggagac ggcaacactt tctgatccag cggaagagaa agcggtagtg
     5521 cgttacgccc gtgagcataa tctcggcaag attattctcg gtcgcccagc ctcgcgccgc
     5581 tggtggcgtc gggaaacgtt tgctgaccga ctggcgcgca tcgcccccga tctcgatcag
     5641 gtgctggtcg cgcttgatga accacccgcc cgcacgatta acaacgcgcc ggatagccgc
     5701 tcttttaaag acaagtggcg ggtacagatt cagggatgcg tggttgccgc cgcgttatgc
     5761 gccgttatca ccttaattgc catgcagtgg ctgatggcgt ttgatgccgc caacctggtg
     5821 atgctgtatc tacttggcgt ggtggtggtg gcgctatttt atggacgctg gccttcggtg
     5881 gttgctaccg tcattaatgt ggtgagtttc gatctctttt ttatcgcccc acgcggcacg
     5941 ctcgccgtct ctgatgtgca atatctgctg accttcgcgg tgatgttaac cgtcgggctg
     6001 gtgatcggga accttactgc tggcgtgcgt tatcaggcgc gggtagcccg ttaccgcgaa
     6061 caacgcacac ggcacttata tgaaatgtcc aaagctctgg ctgtgggccg cagttcgcag
     6121 gatatcgccg ccaccagcga acagtttatt gcctccacgt ttcatgcccg tagccaggtg
     6181 ttgctgcctg atgacaacgg taaattgcag ccgttaacac atccgcaagg aatgacgccg
     6241 tgggacgatg ccatcgcgca gtggagtttt gataaaggtc tgcctgcggg cgcgggcacc
     6301 gacacgttac ccggtgtacc gtaccagatt ttgccgctaa aaagcggcga gaaaacctac
     6361 ggactggtgg tggtggaacc ggggaatctg cgccagttga tgatcccgga acagcagcgc
     6421 ctgctggaga cgtttacgct gttagtcgcc aatgcgcttg agcggctgac gctgaccgcc
     6481 agcgaagaac aggcgcggat ggcaagcgaa cgtgaacaga tccgcaacgc tctgctggcg
     6541 gcgctttcgc atgatttacg cacgccgctc acggtgctgt tcggtcaggc agaaatctta
     6601 acgctcgatc tggcaagcga aggatcgccc cacgcccgcc aggccagcga gattcgtcag
     6661 catgtgctga acacgacccg actggtgaat aatctgctcg atatggcgcg aattcagtcc
     6721 ggcggcttta atttgaagaa agagtggtta acgctggaag aagtggtcgg cagcgcgctg
     6781 caaatgctgg aaccgggttt atcgtcgcct atcaatcttt ctctgccaga accgctgacc
     6841 ttaatccacg ttgacgggcc gctctttgaa cgggtgctga ttaatctgct ggagaacgcg
     6901 gtgaaatacg cgggtgcgca ggccgaaatt ggtatcgatg cccacgttga gggcgagaag
     6961 ctacaactgg atgtctggga taacggcccc ggtcttccgc caggccagga gcagacgata
     7021 tttgataagt ttgctcgcgg gaataaagag tcggcggtac cgggtgtagg gcttgggctg
     7081 gcaatttgtc gggcgattgt ggatgtacac ggcggcacca ttaccgcgtt caaccgaccg
     7141 gaaggtggtg cctgttttcg tgttacactt ccccagcaaa ccgctcctga acttgaagaa
     7201 tttcatgagg atatgtgaca aacgttctga ttgttgaaga tgaacaggct attcgtcgct
     7261 ttctgcgcac ggcgctggaa ggcgacggta tgcgcgtctt tgaggccgaa acgctgcaac
     7321 gcggcttgct ggaagcggct acccgtaagc cagatttgat tattctcgat ctcggcctgc
     7381 ccgatggtga tgggattgag tttatccgcg acctgcgcca gtggagcgcg gtgccggtga
     7441 ttgtgctttc cgcacgtagc gaagagagcg acaaaatcgc cgcgctggat gccggagcgg
     7501 atgattatct gagtaagccg tttggcattg gcgaattgca ggcccgtctg cgcgtcgctt
     7561 tacgtcgcca ctccgccacc gccgcgcccg atccgctggt gaaattttcc gatgttaccg
     7621 tcgatttagc agctcgtgtg atccatcggg gcgaggaaga ggtgcatctc accccgattg
     7681 agttccgcct gctggcagtg ttgcttaata atgcgggaaa agtgctcacc cagcgccagc
     7741 tccttaacca ggtgtggggg ccaaacgcgg tcgaacacag tcattatttg cgtatttata
     7801 tggggcatct gcgacaaaaa ctggaacagg acccggcccg gcctcgccat ttcattact
//
LOCUS       DAZKGF010000197         7790 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZKGF010000197 DAZKGF010000000
VERSION     DAZKGF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.197"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..307
                     /locus_tag="ACHOQV_004379"
     CDS             <1..307
                     /locus_tag="ACHOQV_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632654.1"
                     /translation="YKNIPDRFFITGVNTFTLGNIKLICPTAIVKARIESLKFTVNDI
                     DEYEQKGKKLKKTAMKCIPFDKNDIHTWDSPLSEPIYYEKIKQTIDNSMLKILHVVK"
     gene            375..683
                     /locus_tag="ACHOQV_004380"
     CDS             375..683
                     /locus_tag="ACHOQV_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFP9632655.1"
                     /translation="MQSQTALLLLIIPFPVLVIASPESTQLALALKQLDGVKTSLEHA
                     RTSASNDPQARYFFDYLQAQQDISVVEQGIRRYLAPARAQPRAIQPLSANYQQENPDD
                     "
     gene            676..909
                     /locus_tag="ACHOQV_004381"
     CDS             676..909
                     /locus_tag="ACHOQV_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFP9632656.1"
                     /translation="MTDSQLAAFQAASLSKPETVSLLVLGLFTSVLLLWCAWTLMVAW
                     QGVCRQRLSWQSFGAVAAHSLLLVLVSFWLVLS"
     gene            919..1236
                     /locus_tag="ACHOQV_004382"
     CDS             919..1236
                     /locus_tag="ACHOQV_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFP9632657.1"
                     /translation="MRWFLLLLFATFNALADLPTMEDPSRGQGKGIMETLQNYGFDIV
                     VLLALAICAVGFLVVANNCIATYSEIQSGRKQWKDLGSMAGVGSILLVVIIWLLNQAS
                     DVL"
     gene            1238..1591
                     /locus_tag="ACHOQV_004383"
     CDS             1238..1591
                     /locus_tag="ACHOQV_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFP9632658.1"
                     /translation="MATIPFLPDRLNREPVVWRGLTTSELFLALALGLGGGCAFGILL
                     ALITHYWPLIPGSALAGAALLIQGGGRILARAKRGKPDTWLPQALLAWLERHHLRGAQ
                     LIQHSAVWVVRRSPR"
     gene            1588..2247
                     /locus_tag="ACHOQV_004384"
     CDS             1588..2247
                     /locus_tag="ACHOQV_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFP9632659.1"
                     /translation="MSRFRHALAERDNHILTLRAVIVLLCLCLAGVGYGWYSAPQKLT
                     LWFPPDLRSGSTRPWWEVPPASVYAFTFYIFQQLNRWPDNGEEDYSRNLRSLRAYLTP
                     ACYTRLEHDFQVRRNGGELRDRTRGVYELPGHGYSSLVHEGEKRPFPRVEALSRDSWR
                     VTLDVAVEEYYQNEPVKKALVRYPLQVVRYAIDAEKNPWGLAIDCTDAAPQRLVAEEK
                     P"
     gene            2244..3011
                     /locus_tag="ACHOQV_004385"
     CDS             2244..3011
                     /locus_tag="ACHOQV_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFP9632660.1"
                     /translation="MKPVLFTLLFSFPAFATEVVQWENIPLPVTLHVGQERIVDVGKP
                     VRIGYPATLEGKVRLQSAGGKVFLLANTAFPATRIQLRDTGNGELILLDIHATQGTST
                     LEPMQISYTPQTPATAKTSVPVTASTEPLPVLLVRYAAQNLYAPLRTVEALPGVTPAP
                     VRLAKRITTLLPQQPVTATPLASWQVNATTVTAIRLQNHSGQRITLDPRELQGQFTAA
                     AFQHDWLGPRGLAEDTTVAYLVTEGPVSRALLPEPKP"
     gene            3008..4303
                     /locus_tag="ACHOQV_004386"
     CDS             3008..4303
                     /locus_tag="ACHOQV_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFP9632661.1"
                     /translation="MMPRQSLLLKILLPLIVLVTVWLLLRPASKNTQETSLSRTVAAL
                     TPQELRDLGIDGDTPADTVATLVGQMKQYRRELQAIQTNNESQQAREKRLNAQQATLA
                     SQLREDMRRQQDQMRDELVKSQRGLLDKLDRLMAKRPDDIPPGLGLTPGELPADVQPD
                     IRWLEPLDAPPPVDEKLLRVPLKAADKPPPKPDVKVYTLPQNATLTGSVAMTALIGRV
                     PLNGTVSDPYPFKLLIGEDNLTANGVELPDVAGAVVSGIASGDWTLSCVRGQITSMTF
                     VFHDGIIRTVPQGGKSGNKQQEIGWLSDNQGLPCIPGERKSNAKEYLTSQFLLAGSGA
                     AAQAFANGESTTVVEDGSITSAVTGNNGQYVLGQAIGGGLKETADWMKQHYGQMFDAV
                     YVPPGHKVAVHLTEALSIDYEPEGRQVKYQQASTPGGLE"
     gene            4300..4659
                     /locus_tag="ACHOQV_004387"
     CDS             4300..4659
                     /locus_tag="ACHOQV_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFP9632662.1"
                     /translation="MKFTILILLLLLAGCSTSQEELMPVDPNASMLTLWQQQSGGNAA
                     LLDARAQLRRPPDGPVAQPEADTIRSQFPRLPNPDMVLYVFQHLNSNTPVPGYSTVFP
                     FYTRPQYALPGERLTPP"
     gene            4656..7298
                     /locus_tag="ACHOQV_004388"
     CDS             4656..7298
                     /locus_tag="ACHOQV_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFP9632663.1"
                     /translation="MNERRGLTEQALNTSYRKGPSFVDWLPWVEFLPESQTLLLEDGR
                     SVGAVFELYPVGTEGRSTAFLEQVRDTVTDALQDAFDEHERAPWVVQFFCQDEDDSEA
                     WLASLASYAAPEIRDSAFTREWLTQMANHMVNLTRDEGLFLDNTVTRTRWRATWRRTR
                     MVVYRWLPGLSPDEPSPEETLNNICERLTAGLKAAGISAIRQNEQDIHRWLVRWFNPA
                     PPAEDKKAFWQQLDLPETEPLPVLADFAERLLASEPRSDAANGVWWLDNRPHKVVVVD
                     RLRKAPPPGLLTGETARGDGINALVDLLPGGTIMNLTVVVYPQDKLEQHISRLADRAI
                     GDNTDSEYTRRDCSEVRSFLKDGHKLYRSTLAFYLSAPDTDILFRQQRQLQSLLLNAG
                     LVPVQEQHEVAPLSHWLRWLPMCFDPLRDPRGLYTRLNFVQHIAGLLPLYGRETGTGH
                     PGISYFNRGGTPLCFDPLNKLDRAQNGHLLLLGPTGAGKSATLNAKIAQLMALHRPRL
                     FIIEAGNSFGLMADYAAAHGLTVNKISLRPGSGVTLPLFADAWRLVEQAVTDAEPPED
                     EESDGDDQRDVLGEMEITARLMITGGEAKEDARLTRPDRAMIRQAIIAAAHTCYEEKR
                     QTLTQDIRDALFALSRDNSLPEARRQRAYEMAESINLFCAGFEGELFNREGQMWPEAD
                     ITLVDLATFAREGYEAQLAISYISLINHINNLGERAQHLARPIVNITDEAHIITVNPL
                     LARFLTKGSKMWRKLGIWLWLATQNLADFPDEAKKLLNMIEWWELLVMPPEEIEQVCR
                     FKALTEEQSQLLRSAVKSPGQYTEGVVLSPRIEALFRVVPPALYLALAMTEKHEKADR
                     MRIMKETGCSEVEAAITLSSVISQ"
     gene            7347..>7790
                     /locus_tag="ACHOQV_004389"
     CDS             7347..>7790
                     /locus_tag="ACHOQV_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632664.1"
                     /translation="MTAIACWFNREESDSIWAVSDSRITQQNSRLTDHCPKLFSIPVS
                     VIRSSDVYRIHPQKIFEFGFGFAGSTMIGINVKEMLAVSLARLHEIANNNPEQQISTE
                     AFPSLNEIASLAKNIAEQYMSDIGQSFPQAVRIQMIIFGFCWRTQT"
BASE COUNT         1684 a         2349 c         2236 g         1521 t
ORIGIN      
        1 ctataaaaac atccctgata gattcttcat tactggagtt aacacattta ctttagggaa
       61 tataaaactc atctgcccaa cagcaatagt caaagcaaga atagaaagtt tgaaattcac
      121 tgtaaatgac attgacgagt acgaacaaaa agggaaaaaa ctaaaaaaga cagcgatgaa
      181 atgtattccc tttgataaaa atgatataca tacctgggac tcgccattat ctgaaccaat
      241 ttattacgag aaaatcaaac agaccattga caactctatg ctaaaaattc ttcatgtagt
      301 gaaatgattt tttagctgct tcacctctga agctctccta ctttgctaat cattgagtat
      361 aaggagagct tcaaatgcag tcccagacag cactattact gttaataata cctttcccgg
      421 tactggtaat cgcctccccg gaatccaccc agctcgccct cgcgctgaag caactcgacg
      481 gcgttaaaac ctcgctggag cacgcccgga ccagcgccag caacgatccg caggcgcgct
      541 acttcttcga ctacttacag gcgcagcagg acatctctgt cgttgaacaa ggcattcgcc
      601 gctatcttgc cccggctaga gcacagccgc gtgctattca gcccctgagc gccaactacc
      661 agcaggagaa tcccgatgac tgactcgcaa ctcgccgcct ttcaggccgc cagcctgagc
      721 aaaccggaga ccgtcagcct gctggtgctc ggcctgttta ccagcgtcct gctgctgtgg
      781 tgtgcctgga cgctaatggt cgcctggcag ggcgtctgcc gccagcgtct gagctggcag
      841 agctttggcg cggtggcggc ccattcactg ttactggtgc tagtcagctt ctggctggta
      901 ctgagctgag gaggcatcat gcgctggttt ctgttactgc tgttcgccac atttaacgcc
      961 ctcgccgacc tgccgacgat ggaagatccg tcacgcggtc agggcaaggg cattatggag
     1021 acgctgcaaa actacggttt tgacatcgtg gtgctgctgg cgctggccat ctgtgccgtc
     1081 ggttttctgg tagtggcgaa caactgtatt gctacctatt cggagatcca gagcggacgc
     1141 aagcagtgga aagatctggg ctcgatggca ggcgtcggca gcattcttct ggtggtcatc
     1201 atctggctgc tcaaccaggc ttcggacgtc ctgtgatatg gcaaccatcc cctttctccc
     1261 cgaccgcctc aaccgcgagc ccgtggtctg gcgcggcctc accaccagcg aactgtttct
     1321 ggcgctggct ctcggtcttg gcggcggctg tgcgttcggc attctgctgg ccctgataac
     1381 ccactactgg ccgttgatcc ccggttcggc acttgctggc gcggcattac tgattcaggg
     1441 tggtgggcgg attctggccc gcgcaaaacg cggtaagccc gacacctggc tgccgcaggc
     1501 gctcctcgcc tggctggagc gtcatcatct tcgcggcgca caactgatcc agcacagcgc
     1561 cgtgtgggtg gtgcgcagga gtccacgatg agccgctttc gccatgcgct ggctgagcgc
     1621 gataaccata tcctgacgct gcgggcggtg attgttctgc tgtgcctgtg tctggcgggc
     1681 gtcggctacg gctggtacag cgcgccgcaa aagctcaccc tctggttccc gccggatctg
     1741 cgcagcggca gcacccgccc gtggtgggaa gtgcctcccg ccagcgtcta cgccttcacc
     1801 ttttatatct tccagcagct taaccgctgg ccggataacg gcgaggagga ctattcccgt
     1861 aatcttcgtt cgctgcgtgc ctacctgacg cccgcctgtt acacccggct ggagcacgat
     1921 tttcaggtgc gtcgcaacgg cggtgaactg cgcgaccgca cgcgcggcgt ctatgaactg
     1981 cccggccacg gctacagcag cctggtgcat gagggcgaga agcgcccctt cccgcgcgtg
     2041 gaggcgctga gccgggattc atggcgggta acgctggatg tggcggtgga agagtactac
     2101 cagaacgagc cggtgaaaaa agcgctggtg cgctacccgt tgcaggtggt gcgctacgcc
     2161 atcgatgcgg agaaaaatcc gtggggactg gcgatcgact gtaccgatgc cgcgccgcag
     2221 cgtctggtgg cggaggagaa accatgaagc cagtcctctt tactttattg ttcagtttcc
     2281 ccgccttcgc cacggaagtg gtgcagtggg aaaacatccc gctgcccgtc acgctgcatg
     2341 tcgggcagga gcgaattgtt gacgtgggta aaccggtgcg cattggctat cccgccacgc
     2401 tggaaggcaa agtgcgtctg caaagcgcag gcggcaaggt cttcctgctg gcgaacacgg
     2461 cgttcccggc cacccgcatc cagttacgtg ataccggcaa cggtgagctg atcctgctgg
     2521 atattcatgc cacccagggc acttcgacac ttgaaccaat gcaaattagc tatacgccgc
     2581 agacgcctgc cacagcaaaa acatccgtcc cggtgacagc atcaacagaa cctctgcccg
     2641 tcttgctggt gcgctacgcc gcgcaaaatc tgtatgcgcc gctgcgcacc gtcgaagccc
     2701 tgcccggcgt cacacctgcg cccgtccggc tggcgaaacg catcaccact ttactgccgc
     2761 agcagccggt taccgccacc ccgctggcgt catggcaggt caacgccacc accgtcaccg
     2821 ccatccgcct gcaaaatcac agcggacagc gcatcacgct cgatccgcgc gagcttcagg
     2881 ggcagttcac cgctgccgcg ttccagcatg actggctcgg cccgcgtggt ctggcagaag
     2941 ataccaccgt ggcgtacctg gtgacggaag gtccggtttc ccgcgccctg ctgccggagc
     3001 ccaaaccatg atgccgcgcc agagcctgct gctgaaaatc ctgctgccgc tcatcgtact
     3061 ggtaacggta tggctgctgc tgcgcccggc cagcaaaaac acgcaggaga cttccctgtc
     3121 ccgcaccgtg gcggccttaa cgccgcagga actgcgcgat ctgggcatcg acggcgacac
     3181 gcccgccgac accgtcgcca cgctggtcgg ccagatgaaa cagtaccgcc gcgaacttca
     3241 ggcaatccag acaaacaacg aaagccagca ggcgcgcgaa aaacggctta acgcacagca
     3301 ggccacgctg gcctctcagc ttcgggaaga catgcgccgc cagcaggacc agatgcgcga
     3361 tgaactggtg aaaagccagc gcgggctgct ggataagctc gaccgtctga tggccaagcg
     3421 cccggacgat atcccgcccg gtctggggct gacgcccggc gaactacctg ccgacgttca
     3481 acccgatatc cgctggctgg agccgctgga tgcgcctcca cctgtggatg aaaagctgct
     3541 gcgcgtccct ctgaaagcgg ccgacaaacc accgccgaaa cctgacgtga aggtctacac
     3601 cctgccgcag aacgccacgc tgaccggatc ggtggcgatg acggcgctga ttggccgcgt
     3661 gccgcttaac ggcaccgtca gcgatcccta cccgtttaaa cttctgattg gcgaggacaa
     3721 cctcaccgcc aatggcgttg agctgccgga cgtggcgggc gcggtggtct ccggcatcgc
     3781 ctcgggagac tggacgctct cctgtgtgcg cgggcaaatc acctcgatga cctttgtgtt
     3841 tcacgacggc atcatccgca ccgtcccgca gggcggcaaa tccggcaaca aacagcagga
     3901 gatcggctgg ctttcggata accaggggct gccatgtatt cccggcgagc gtaaaagcaa
     3961 tgcgaaggag tacctgacct cgcagttcct gctggcgggt tccggtgcgg cggcgcaggc
     4021 cttcgccaat ggcgaaagca ccacagtggt cgaggacggc agcatcacca gcgcagtgac
     4081 gggcaacaac ggtcagtacg tgctcggcca ggctattggc ggcgggctga aagagaccgc
     4141 cgactggatg aaacagcact acggccagat gttcgacgcc gtgtacgtgc cgccgggcca
     4201 caaggtggcg gtgcatctca ccgaagcgct gtctatcgac tacgagccgg aaggccgtca
     4261 ggtgaaatac cagcaggcca gcacaccggg aggtctggaa tgaaatttac catccttata
     4321 cttctgttgt tactggccgg atgcagcacc tcgcaggagg aactgatgcc tgtcgacccc
     4381 aatgccagca tgctgacgct gtggcagcag caaagcggcg gcaacgcggc tctgctggat
     4441 gcccgcgccc agctacgccg cccgccagat ggtccggtgg cgcagcctga ggcagacact
     4501 atccgcagcc agtttccgcg cctgcccaac ccggatatgg tgctttacgt cttccagcac
     4561 ctcaacagca acacgccggt acccggctac agcaccgttt tccccttcta tacccgtccg
     4621 cagtatgcgt tgccgggcga acgcctgacg ccgccatgaa tgaacggcgc ggcctgactg
     4681 aacaggcgct gaataccagc taccgcaagg gcccgtcatt tgtcgactgg ctgccgtggg
     4741 tggagttttt gccggagtcg cagacgctgc tgctggaaga tggccgctcg gtaggtgccg
     4801 tatttgagct atatcccgtt ggcaccgaag gccgctcaac ggcgtttctg gaacaggtcc
     4861 gcgataccgt caccgatgcc ttacaggatg cgtttgatga gcatgagcgc gcaccgtggg
     4921 tggtacagtt tttctgtcag gatgaggacg acagcgaggc gtggctggca tcacttgcct
     4981 cttacgccgc accggaaatc cgcgactccg cctttacccg cgagtggctc acacagatgg
     5041 caaaccatat ggtgaatctc acccgcgacg agggactgtt tctcgataac accgtcaccc
     5101 gtacccgctg gcgggcgacg tggcggcgca cgcggatggt ggtgtaccga tggcttcctg
     5161 gactttcacc ggatgaaccg tcgccggaag agacgctgaa taatatctgc gaacgcctta
     5221 ccgccgggct aaaagcggcg ggtatcagcg ctatccgcca gaatgagcag gacattcacc
     5281 gctggctggt gcgctggttt aatcccgcgc cgcctgctga ggacaaaaaa gccttctggc
     5341 agcagcttga cctgccggaa actgaaccgc tgccggtcct ggccgatttc gccgaacgcc
     5401 tgctggcgag tgaaccccgt tcggatgcgg caaacggcgt ctggtggctg gataaccgcc
     5461 cgcataaagt ggtggtggtt gaccgcctgc gcaaagcgcc gccgcccggc ctgctgaccg
     5521 gggaaaccgc gcgcggagac ggcattaacg ccctggtcga cctgctgcct ggcggaacca
     5581 tcatgaacct gaccgtggtg gtgtatccgc aggacaagct ggagcaacac atcagccgcc
     5641 tggccgaccg ggcgattggc gacaataccg attcggaata cacccgccgc gactgcagcg
     5701 aggtgcgaag cttcctgaag gacggccata aactctaccg cagcacgctg gcgttttatc
     5761 tcagcgcgcc ggacaccgat atcctgttcc gccagcagcg tcagctacag agcctgctgc
     5821 tcaacgccgg gctggttccg gtgcaggagc agcacgaagt cgccccctta agccactggc
     5881 tgcgctggct gccgatgtgc ttcgatcccc tgcgcgaccc gcgcgggctg tatacccggc
     5941 tcaactttgt gcagcatatc gccgggctgc tgccgctgta cggacgggaa accggcaccg
     6001 ggcatccggg gatcagctac ttcaaccggg gcggcacgcc gctgtgcttc gatccgctga
     6061 acaagctgga tcgtgcccag aacggacacc ttttacttct tgggccaacc ggagcgggca
     6121 aatccgccac cctcaacgcg aaaatcgcgc aactgatggc gctacatcgt ccgcgcctgt
     6181 ttatcatcga ggccggaaac tcatttggcc tgatggccga ctacgccgcc gcccacggcc
     6241 tgacggtgaa taaaatcagc ctgcgccccg gttctggcgt caccctgccc ctgttcgccg
     6301 atgcgtggcg actggtggaa caggcagtta ccgacgccga accgccagag gacgaagaga
     6361 gcgacggcga tgaccagcgc gacgtgctgg gtgaaatgga gatcaccgcc cggttgatga
     6421 ttaccggtgg tgaagcgaaa gaggatgcgc gcctcacccg gccggatcgg gcgatgatcc
     6481 gccaggcgat tatcgctgcc gcgcacacct gctacgagga aaaacgacag acgctgaccc
     6541 aggatatccg tgacgcactg ttcgcccttt cccgcgacaa cagtctgccg gaagcccgcc
     6601 gccagagggc gtatgaaatg gcggaatcca tcaacctgtt ctgcgccggg tttgaaggcg
     6661 aactgtttaa ccgtgaaggc cagatgtggc cggaggcgga tatcacccta gtggatctcg
     6721 ccacctttgc ccgcgaaggc tatgaagcgc agcttgccat cagctatatc tcgctgatta
     6781 accatatcaa taacctcggc gagcgcgccc agcatctggc gaggcctatc gtcaatatca
     6841 ccgatgaggc gcatatcatc actgttaatc ccttattggc gcgctttctc accaaaggct
     6901 cgaagatgtg gcgcaagctc ggtatctggc tgtggctcgc cacccagaac ctggcggatt
     6961 ttcctgatga ggcgaagaag ctgctcaata tgatcgaatg gtgggaacta ctggtgatgc
     7021 cgccggaaga gatcgagcag gtctgccggt ttaaagcgct tacggaagaa cagagccaac
     7081 tgctgcgctc agcggtaaaa tcaccggggc agtataccga gggcgtggtg ctttcaccgc
     7141 gtatagaggc actgtttcgg gtggtgccgc cagcgctcta tctggcgctg gcgatgacag
     7201 agaaacatga aaaggccgat aggatgcgga ttatgaagga gacagggtgt agtgaagtgg
     7261 aggcggcgat aaccctaagc tcggtaatct cgcaataaaa atatgctaag ctattcgcat
     7321 taatgttaaa taactggaaa aaccttatga ccgcaattgc atgttggttt aatagagagg
     7381 aaagcgatag tatttgggca gtcagcgact caagaatcac tcagcaaaat agcagactaa
     7441 ctgaccattg tccgaaatta ttctcaatcc ccgtatcagt tattcgctct tcggacgttt
     7501 atcgaattca cccgcaaaaa atattcgaat ttggcttcgg atttgcagga agtacaatga
     7561 ttggaattaa tgtcaaagaa atgcttgcag tatcattagc aagacttcat gagattgcga
     7621 acaataatcc agaacaacaa atatcgaccg aagcattccc aagtcttaat gaaatagctt
     7681 cattagcaaa aaatattgcc gagcaatata tgtctgatat cggccaatcc tttccccaag
     7741 cagtaagaat acagatgata atttttggtt tttgctggag aacacaaacc
//
LOCUS       DAZKGF010000198         7701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZKGF010000198 DAZKGF010000000
VERSION     DAZKGF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.198"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..222
                     /locus_tag="ACHOQV_004390"
     CDS             <1..222
                     /locus_tag="ACHOQV_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632665.1"
                     /translation="ETRLQASAPAMPAIEALNAFIDKGDYLKSLYVNKTRTAAEQKRL
                     NEILRAQVISGLKASEGLAKLLIASGIML"
     gene            233..541
                     /locus_tag="ACHOQV_004391"
     CDS             233..541
                     /locus_tag="ACHOQV_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632666.1"
                     /translation="MNDVQNRRILALLDMLYNTLGNKTTCNVCHKYFILILTVMNNLT
                     INYGTETSPELSGISYEDANALITELILFIEDVDAENHEAYQGHLNEITRILNFAIAA
                     "
     gene            complement(593..1183)
                     /gene="cobO"
                     /locus_tag="ACHOQV_004392"
     CDS             complement(593..1183)
                     /gene="cobO"
                     /locus_tag="ACHOQV_004392"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP9632667.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGIVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(1180..1938)
                     /locus_tag="ACHOQV_004393"
     CDS             complement(1180..1938)
                     /locus_tag="ACHOQV_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFP9632668.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2158..3207
                     /gene="sohB"
                     /locus_tag="ACHOQV_004394"
     CDS             2158..3207
                     /gene="sohB"
                     /locus_tag="ACHOQV_004394"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFP9632669.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3243..3494)
                     /locus_tag="ACHOQV_004395"
     CDS             complement(3243..3494)
                     /locus_tag="ACHOQV_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFP9632670.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            3874..6471
                     /gene="topA"
                     /locus_tag="ACHOQV_004396"
     CDS             3874..6471
                     /gene="topA"
                     /locus_tag="ACHOQV_004396"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP9632671.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            6681..7655
                     /gene="cysB"
                     /locus_tag="ACHOQV_004397"
     CDS             6681..7655
                     /gene="cysB"
                     /locus_tag="ACHOQV_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFP9632672.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEIMFKDIKLPEK"
BASE COUNT         2015 a         1803 c         1994 g         1889 t
ORIGIN      
        1 gaaaccagac tacaggcatc ggcacctgcc atgcctgcga ttgaggcttt gaatgccttt
       61 atagataagg gtgattatct gaaaagctta tacgttaata aaacacgcac ggcagctgaa
      121 caaaaaagat taaatgaaat attacgcgca caggtcatta gtgggttaaa ggcgtctgag
      181 gggctggcaa aattacttat tgccagcgga ataatgttat aggaggcttt ttatgaacga
      241 tgtgcaaaac cgtagaatac tggcattact tgatatgctg tataatactt tgggtaataa
      301 aaccacatgt aatgtttgtc ataaatattt tatccttatc ctcacggtga tgaacaatct
      361 gacgattaac tacggtactg aaacttcgcc agagttaagc ggaatcagtt atgaagatgc
      421 caatgcgtta attactgaac tgatattatt tattgaagat gttgacgctg agaatcatga
      481 agcatatcag ggacatttga atgagattac acgtattctg aatttcgcta ttgccgcttg
      541 aatataatca tgattaaagc acctcaagag gcttcttttg aggtgctttc acttagtagt
      601 caatccctat ctgcgctttt acaccggcat caaacgcatg tttcacgggg cgtaattcac
      661 tcaccgtatc cgccagttca agaatatccc gatgacagcc tcgacccgtg ataatcaccg
      721 tctgttgatg tggacgttca tttaacgcct gcaccacttc ttccagtggc aaatagtcat
      781 acgccaccat atacgtcagt tcatcgagca aaaccatatc cagtgaagga tcggccagca
      841 ttcgtttggc atgttgccag acttcacggc aggccgcggt atcagattcg cgattttgtg
      901 tatcccaggt aaagcccgtt gccatcacct gaaactcaac gccatgtggc tccagtagat
      961 tgcgctcgcc attaggccag gtgcctttaa taaactgcac gatacccact tttttgccat
     1021 ggcctactgc acgagttgcc gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg
     1081 taaagacgat gataataccg cgctcatcct gggcctgggc cacacgagca tcgacttttt
     1141 ctttcactcg ctgctgacgc tgttggtagc gttcatcact cattgggaaa ttcctggttt
     1201 acggcccggt tgggcgtcaa aggtcatgcc ggttttacgg cggctgtcat cgcccatcag
     1261 ccagaggtag agcggcatga tatcagcggg tgttttaagt ttctgtggat cttcggtcgg
     1321 gaaggcgctg gcacgcattg cggtgcgcgt accgcctggg ttaatgcagt tgacgcgcag
     1381 gcgctgctga tattcatcgg ccagtacctg catcatccct tcggtggcaa atttcgacgc
     1441 tgcatatgcg ccccagttgg ctcgtccctg acgtccaacg cttgatgaag taaagaccag
     1501 tgaaccggcg tccgatttga gtaataaagg aagcagtgcc tgggtgagca taaaggtggc
     1561 attaacgttg acctgcatga cgtcctgcca gacctgcgga ttttgttcgc tcattgggca
     1621 aacatcgccg agcaatccgg cattatgcaa aacaccatcc agacgcggat aattaacggc
     1681 aatgcgctgt gccagttgtt ggcaatcttc ggacgtgcag gtcagcaaat cgagaataaa
     1741 ccactgtggc tgacgtccag tttcttcgtt tatgtggctg gctacctgac gtaatttttc
     1801 ttcattacgg cccaacagaa tcactgtcgc accatagcgt gcatacgtca tcgcggcttc
     1861 acggccaata ccatcgctgg ctcccgtcac caggataatg cgatcattga gtaaatcttg
     1921 ttttggctgg taatgcatgg ctactcctca acgacgttgt ctgtcgcgct gccagtgtac
     1981 tttatgactt tggggcttta tgcctgaaac agaagggcat ttcaatcagc ctggggcgat
     2041 aacaccgcaa aatggatact ttgtcatact ttcgctgcaa taacatctct gcgagacggc
     2101 ttaacatgcc tgttgtaaac tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg
     2161 gaattgttgt ctgaatatgg tttgtttttg gcgaaaatcg ttaccgttgt gctagcgatt
     2221 gcggcgattg ccgccattat tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag
     2281 ttacgggtca acaatctcag cgaacagtat aaggagatga aagaagaact ggccgcggcg
     2341 ctgatggact cacatcagca aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa
     2401 gaagcgaaag cagcaaaagc gaaagccaaa ctgggcgagg tggcaactga cagtaaacct
     2461 cgcgtctggg tgctggattt taaaggcagc atggacgccc atgaagtgaa ctcgctacgt
     2521 gaagagataa cggcggtact cgcagcattc aaaccgcagg atcaggttgt gctccgtctg
     2581 gaaagccctg gtggcatggt gcatggttac gggttggcgg cttcgcagct gcagcgtctg
     2641 cgtgataaaa acattccttt aactgtaacg gtagacaaag tcgctgccag cggcggttac
     2701 atgatggcct gtgtggcgga caaaattgtt tccgcaccgt ttgctattgt gggttccatt
     2761 ggcgtggtgg cgcaaatgcc caactttaac cgcttcctga aaagcaaaga tattgatatc
     2821 gaactgcaca ccgccgggca gtataagcgc acgctgacct tgctgggtga aaacaccgaa
     2881 gaagggcggg agaaattccg cgaagagctg aacgaaacgc atcagttatt taaagatttt
     2941 gtgaagcgta tgcgtccgtc tctggatatt gaacaggtgg caacgggtga acactggtac
     3001 ggacaacagg cggtagagaa aggcctggtt gatgaaatca acaccagtga tgaagttatt
     3061 cttagcctga tggaaggccg tgaagtggta aatgtacgct atatgcagcg taaacgactc
     3121 attgaccgat tcaccggcag cgcggcagag agcgccgatc gattgttgct acgctggtgg
     3181 cagcggggtc aaaagccatt gatgtaaaag acaaacgcga gggtaagacc tcgcgttttg
     3241 ctttaatcaa ccaggtgata tttttctgaa agcacatggg ccaggtgttt gaacatatta
     3301 aacaccgcgg tgcttttggc tgttggcaat ccttgttcat ctaaaaagta gtcgccggta
     3361 aataccagca cgccattacg ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc
     3421 tcatgctcac gaatgatttt gttggcctct ttcaacagcg tctcgcggtc gatgggttgt
     3481 gtctctttgt tcattattta ctccttaaac aaggacatta gtctacgcca ggcatggctt
     3541 gcagacaaat ataccacgct ggtggcaaga gcgccttact ggcaactttg gattttgcat
     3601 gctaataaag ttgcgtatcg gattttatca ggtacagtgt gacgctttcg tcaatctggc
     3661 aatagatttg cttgacattc gaccaaaatt ccgtcgtgct atagcgcctg taggccaaga
     3721 cctgttaact cagtcacctg aattttcgtg aacagagtca cgacaagggg ttgatatccg
     3781 cagagagcga gtccatatcg gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc
     3841 attcttggaa gaatcaaatt aggtaaggtg aatatgggta aagctcttgt catcgttgag
     3901 tccccggcaa aagccaaaac gatcaacaag tatctgggta gtgactacgt ggtgaaatcc
     3961 agcgtcggtc acatccgcga tttgccgacc agtggctcag ctgccaaaaa gagtgccgac
     4021 tctacctcca ccaagacggc taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac
     4081 cgtatggggg ttgacccgtg gcacaattgg gaggcgcact atgaagtgtt gcctggtaaa
     4141 gagaaggtcg tctctgaact gaaacaactg gctgaaaaag ccgaccacat ctatctcgca
     4201 accgaccttg accgcgaagg ggaagccatt gcatggcacc tgcgggaagt gattggtggt
     4261 gatgatgcgc gctatagccg agtggtgttt aacgaaatta ctaaaaacgc gatccgccag
     4321 gcatttaaca aaccgggtga gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc
     4381 tttatggacc gcgtggtggg gtatatggtt tcgccgcttc tatggaaaaa gatcgctcgt
     4441 ggtctgtctg ccggtcgtgt gcagtcggtg gcggttcgcc tggtggtcga gcgtgagcgt
     4501 gaaattaaag cgttcgtacc ggaagagttc tgggaagtcg atgccagcac gaccacgcca
     4561 tctggtgaag cgttggcgtt gcaggtgact catcagaacg acaaaccgtt ccgtccggtc
     4621 aacaaagaac aaactcaggc tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg
     4681 gaacgtgaag acaaaccgac aaccagtaaa cctggcgctc cttttattac ctctacgctg
     4741 caacaagctg ccagcacccg tcttggattt ggcgtgaaaa aaaccatgat gatggcgcag
     4801 cgtttgtatg aagcaggcta tatcacttac atgcgtaccg actccactaa cctgagtcag
     4861 gacgcggtaa atatggttcg cggttatatc agcgataatt ttggtaagaa atatctgccg
     4921 gaaagtccga atcagtacgc cagcaaagaa aactcacagg aagcgcacga agcgattcgt
     4981 ccttctgacg tcaatgtgat ggcggaatcg ttgaaggata tggaagcaga tgcgcaaaaa
     5041 ttgtaccagt taatctggcg tcagttcgtt gcctgccaga tgaccccagc gaaatatgac
     5101 tccacgacgc tgaccgttgg tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg
     5161 cgctttgatg gctggacaaa agtgatgcct gcactgcgta aaggcgatga agatcgtatc
     5221 ttaccggcag tcgataaagg cgatgctctg acgctcgttg aactgacacc agcccagcac
     5281 tttaccaaac cgccagcccg tttcagtgaa gcatcgctgg ttaaagaact ggaaaaacgc
     5341 ggtatcggtc gtccgtctac ctatgcgtcg atcatttcga ccattcagga tcgtggctat
     5401 gtgcgtgtag aaaaccgtcg tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc
     5461 cttgaagaaa atttccgcga gttaatgaac tacgacttta ccgcgcagat ggaaaacagc
     5521 cttgaccagg tggcaaatca cgaagcagag tggaaagctg tactggatca cttcttctcg
     5581 gatttcaccc agcagttaga taaagctgaa aaagatccgg aagagggggg gatgcgcccg
     5641 aaccagatgg ttctgaccag catcgactgc ccgacctgtg gtcgcaaaat ggggattcgc
     5701 acagcgagca ccggggtatt ccttggctgt tccggctacg ctctgccacc gaaagagcgt
     5761 tgcaaaacca ccatcaacct ggtgccggaa aacgaagtgc tgaacgtgct ggaaggcgaa
     5821 gatgctgaaa ccaacgcgct gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg
     5881 gacagctatc tcatcgatcc gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc
     5941 gacggttacg agatcgaaga gggcgaattc cgcattaaag gttatgacgg cccgatcgtt
     6001 gagtgtgaaa aatgtggctc tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg
     6061 gcctgcacta acgaagagtg taaaaacacg cgtaagattt tacgtaacgg cgaagtggca
     6121 ccaccgaaag aagatccggt gccattaccg gagctgccgt gcgaaaaatc agatgcttat
     6181 ttcgtgctgc gtgacggtgc tgccggtgtg ttcctggcgg ccaatacctt cccgaaatcg
     6241 cgtgaaacac gtgcgccact ggtggaagag ctttatcgct tccgcgaccg tctgccggaa
     6301 aaactgcgtt atctggccga tgcgccacag caggatccgg aaggtaataa gaccatggtt
     6361 cgctttagcc gtaaaaccaa acagcaatat gtctcttcgg aaaaagacgg aaaggcgact
     6421 ggctggtcag cattttatgt tgatggcaaa tgggttgaag ggaaaaaata acctttaatt
     6481 ctgtcaggtt tttataaaca aagggtcgcg aaagcggccc ttttttattg catattattt
     6541 tttcttcaca cctatacact aaggctataa atgatatagt ggttatagtt agcacttttt
     6601 ttattattaa atcgtattag tcacccgcca ggtgtgacga aaaaacgatg ttctgatggc
     6661 gtctaagtgg atggtttaac atgaaattac aacaacttcg ctatattgtt gaggtggtca
     6721 atcataacct gaatgtctca tcaacagcgg aaggacttta cacatcacaa cccgggatca
     6781 gtaaacaagt cagaatgctg gaagacgagc taggcattca aattttttcc cgaagcggca
     6841 agcacctgac gcaggtaacg ccagcagggc aagaaataat tcgtatcgct cgcgaagtcc
     6901 tgtcgaaagt tgatgccata aaatcggtcg ccggagagca cacctggccg gataaaggct
     6961 cgctgtatat cgccaccacg catacccagg cacgctacgc gttaccaaac gtcatcaaag
     7021 gctttattga gcgttatccc cgcgtttctt tgcatatgca ccagggctcg ccgacacaaa
     7081 ttgctgatgc cgtctctaaa ggcaatgctg atttcgctat cgccacagaa gcgttgcatc
     7141 tgtatgaaga tctagtgatg ttaccgtgct accactggaa tcgggctatt gtagtcactc
     7201 cggatcaccc gctggcaggc aaaaaagcca ttaccattga agaactggcg caatatccgt
     7261 tggtgacata taccttcggc tttaccggac gttcagagct ggatactgcc tttaaccgcg
     7321 cagggttaac gccgcgtatc gtcttcacgg caacggatgc tgacgtcatt aaaacttacg
     7381 tccggttagg gctgggggta ggggtcattg ccagcatggc ggtggatccg gtcgccgatc
     7441 ccgacctggt gcgcgttgat gctcacgata tcttcagcca cagtacaacc aaaattggtt
     7501 ttcgccgtag tacttttttg cgcagttata tgtatgattt cattcagcgt tttgcaccgc
     7561 atttaacgcg tgatgtcgtt gatgcggctg tcgcattacg ctctaatgaa gaaattgaga
     7621 tcatgtttaa agatataaaa ctgccggaaa aataatttct tgcgttattt tcggcacctt
     7681 ttatgtagcg aaggtgccgg a
//
LOCUS       DAZKGF010000199         7655 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZKGF010000199 DAZKGF010000000
VERSION     DAZKGF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.199"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..621
                     /locus_tag="ACHOQV_004398"
     CDS             <1..621
                     /locus_tag="ACHOQV_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFP9632673.1"
                     /translation="EYTAQRANCQTFTLGSLSPDTTPAPRKPESFLKRLFSAGQTDNT
                     DTSKPDTGEDAKMDEIKALLEKLITLVEGGSAAAAGEGTADTPEEAKAEVAEIAAEIA
                     DAAAEVAELAEEVEAAPEDEVKAEAFSAAKAKLEDAIKAFSAITPEKGERYRRFAARR
                     RDDTDKEEIKAIAEKLNDLTTKLSATGITPRPAGTPAESEKPFDFV"
     gene            633..1661
                     /locus_tag="ACHOQV_004399"
     CDS             633..1661
                     /locus_tag="ACHOQV_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP9632674.1"
                     /translation="MQLNQKTESLLRKYAAGLGAANGVDDVTRYFALSDPKETALRDA
                     IMHSDEFLSKLPNVLDVEQVTGQVVTTGVPGLHTGRKKNARFTLPLGVTGNEYKLAET
                     DSGAHLLYTTLVTWANAGSENEFFNRIQNFVNRQIALDILRIAFNGTSVAEDTDPVTN
                     KNGEDVNTGWHAIVKGRSAKQIVEAGPTLGKGGDFIGLDAAVADLVHTLIDESYRGHP
                     DLVVLVAPDLVAQDKVTLLNAIDAPTEKVAAQLIGRQIAGLTAYTPPFMPEGRLIVTT
                     LANLHIYTQRGTRQRRAEWNDDRKRFENNYLRMEGYAVEHDEIYAAYDKLTIKGAEAA
                     EEPAPGGA"
     gene            1679..2377
                     /gene="gpM"
                     /locus_tag="ACHOQV_004400"
     CDS             1679..2377
                     /gene="gpM"
                     /locus_tag="ACHOQV_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFP9632675.1"
                     /translation="MTPCQRHRAKIRTQEALERREALTASPVSFHLLRAELDRDVARL
                     RSLPVREERLAFKRDILLPRWLPVAERYIADGKRHACPVLVYCIIWLFDTGDLSRALD
                     WADIAISEGQATPDNFRSTLPAFVADTVLQWATDNSEAGHSIEPYFSRTFRNVTTTWR
                     LHEEIRAKWFRFAGLYLLRDEAGKPRATAINDTAVLEKADAYLAQAAALSRTAGVKSI
                     RARIRARISALNAA"
     gene            2485..2958
                     /locus_tag="ACHOQV_004401"
     CDS             2485..2958
                     /locus_tag="ACHOQV_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP9632676.1"
                     /translation="MSEPSFSVSGKPLKSTPQTVTNGEPFWPDLDLAELQGSRTLPAD
                     LPDDTAAMALLAAVAEVNTTLAPVVAHWKAQGHERAADVPGARLGDETQLVAQYKKAV
                     YARAKADLMGEFATIGRRETYPGQESEDTRATLLAEASLVMRNMLGHRRAGVHII"
     gene            2955..3428
                     /locus_tag="ACHOQV_004402"
     CDS             2955..3428
                     /locus_tag="ACHOQV_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9632677.1"
                     /translation="MNQLESLTAFITENLPPDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRMQEDGPVPYRGKRWTLVNPEIWTAETMDLFVSRSRE"
     gene            3425..4135
                     /locus_tag="ACHOQV_004403"
     CDS             3425..4135
                     /locus_tag="ACHOQV_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632678.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRRRLLWRIARHGIIAAA
                     RRHQRDQAAPDGTAWAPRKRGKGKMFRKLPRLLAVREMPGQDAVKVYFRNGNHTSGRS
                     RISAGAVGWVHHNGAEIRVSADSFSGQDQSGQTATRRQAKRLRELGFKVRQKGRWRKA
                     SMKYLLTLSRAQAGLLITIMEDQIIAGPRQQKARTWTITLPSRVFLGVTNEEFSHILA
                     RQMNAMGYGLDVDAQDIR"
     gene            4147..5292
                     /locus_tag="ACHOQV_004404"
     CDS             4147..5292
                     /locus_tag="ACHOQV_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 domain-containing protein"
                     /protein_id="HFP9632679.1"
                     /translation="MSWPNVTVSHQNRFNGATREVERTLLFVGYGKKNTGNTLSVSPE
                     TDLDDVLGPDESLLKSTLTAAIANGGQNWFAYVHVLSEPKPPAPEGGDANAAWVDAVK
                     KAQTIASAEGVVIAIDITAKDAVNRATETRALLQSAYGRFVWFMLCVAGPGKDEAWAA
                     YVTRISAIQDGVAAPGVMVVPRLWGNEPGVLAGRLCNPSVTVADSPARVATGALVAMG
                     NDEIPQDGKKQPLELATLRSLEGKRFSVPMWYHDFDGLYWSDGRTLDVEGGDYQVIEN
                     VRVVDKVSRRVRLRAIARLADRALNSTPGSIAASQTYFSRTLREMALATEINGVPFPG
                     EVKPPQDGDITIVWTSSTHVQIYIVVRPYESAKAIGVSIELDTALEI"
     gene            5294..5755
                     /locus_tag="ACHOQV_004405"
     CDS             5294..5755
                     /locus_tag="ACHOQV_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HFP9632680.1"
                     /translation="MAGNERISGGAFDINYDGIMIHVESATVTITDNSAVAQTRGVPN
                     GYTRGSVSADVEVEVDAQNFKKFGAVARSAGSWRAIPEKDFLFYANNGSEEEKIEVFG
                     CVPMLSDVVNISTGEAGKTTKKIKFMVTSPDFISIDGVPYLSARDTRDLKG"
     gene            5760..6065
                     /locus_tag="ACHOQV_004406"
     CDS             5760..6065
                     /locus_tag="ACHOQV_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFP9632681.1"
                     /translation="MPDGQTSLLAKLIIIGAAIGLGRLMVSRDRITARLLIGRMIMGS
                     AVAPVAAIPLFKFPDMPELVVVGLACALGIMGSAFIEECFCRLADRYLKQKERRNDP"
     gene            6055..6807
                     /locus_tag="ACHOQV_004407"
     CDS             6055..6807
                     /locus_tag="ACHOQV_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP9632682.1"
                     /translation="MTHKPYISPSLSLYHAQAHDVLRMLPENSVDALITDPPYSSGAT
                     HKAGRTCQGSHAKYINDEDLHRFEGFEGENMDARSWEYWTHLWMTQAWRTVRPGGYAL
                     VFTDWRQLPALTDAFQAAGFTWRGIVAWNKGRASRTPHTGYFRHQCEYVVWGSKGALG
                     KSPSGPFDGCITHHTRPQEKQHPTGKPESLMDELVRVCEPGGLILDPFMGSGTTGVAA
                     LRRGCRFTGIELNAHYFSVAQARLTGTEQRSD"
     gene            6807..7145
                     /locus_tag="ACHOQV_004408"
     CDS             6807..7145
                     /locus_tag="ACHOQV_004408"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFP9632683.1"
                     /translation="MKLSEKQQLFAVMIADLIHWAQEHGYRLTFGEAYRTPEQAALNA
                     KSGKGIRNSLHTLRLAVDFNLFINGEYQADTDAYRPLGEYWESIGGTWGGRFSRADGN
                     HFSLEHNGVK"
     gene            7142..7528
                     /locus_tag="ACHOQV_004409"
     CDS             7142..7528
                     /locus_tag="ACHOQV_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632684.1"
                     /translation="MMKQLAGCLVLLFAAMWAGWLAHDWHDARVALAEQEAMEATRRA
                     VTEAGQQSARILEKRLEALKGNEIHTERVIHTETVRPVFRNVCATDDYVRLFNARAEA
                     AERTLAGKPADGMPEDTAETGRPDRR"
BASE COUNT         1764 a         2253 c         2309 g         1329 t
ORIGIN      
        1 gaatacacgg cacagcgggc gaactgccag acgttcacgc tgggcagcct gtcaccggac
       61 accacccctg ccccccgtaa acctgagtca ttcctgaaac gcctgttcag cgcaggacag
      121 accgacaaca ccgataccag taagcctgat acaggcgagg acgcaaaaat ggacgaaatt
      181 aaagcactgc ttgaaaagct gatcaccctg gtggaaggcg gcagcgccgc tgccgctggc
      241 gagggcacag cagacacacc ggaagaagca aaagccgaag tcgcggaaat cgccgccgaa
      301 atcgccgatg ccgccgcaga ggtggcagag ctggcagaag aggtggaagc cgccccggaa
      361 gatgaggtta aggctgaagc cttcagcgcc gcgaaagcga agctggaaga cgcgattaaa
      421 gcgttcagtg ccatcacacc ggaaaaaggc gaacgttacc gccgctttgc cgcccgtcgc
      481 cgggacgaca ccgacaaaga agaaatcaaa gccattgccg aaaaacttaa cgacctgacc
      541 acgaagctgt cagccaccgg catcacgcca cgcccggctg gcacaccggc agaaagcgaa
      601 aaaccgtttg actttgttta aggatacgca acatgcaact gaaccagaaa accgaatcac
      661 tgcttcgtaa gtacgctgcc gggctgggtg cggcaaacgg ggttgacgac gtcacacgct
      721 attttgcgct gtctgacccg aaggagaccg cgctgcgtga cgccatcatg cactccgacg
      781 agtttttatc aaaactgccg aacgtgctgg atgttgaaca ggtgacaggc caggtggtta
      841 ccaccggcgt tccgggcctc cataccggac gtaaaaaaaa tgcccgcttc acccttccgc
      901 tgggtgttac cgggaatgaa tacaagctgg ctgaaaccga ttccggcgcc cacctcctgt
      961 acaccacact ggtcacctgg gcgaacgcag gaagtgaaaa cgagttcttt aaccgcattc
     1021 agaatttcgt taacagacag attgcgctgg atatcctgcg catcgccttt aacggtacca
     1081 gcgtggccga agataccgac ccggtaacca acaaaaacgg cgaggacgtc aacaccggct
     1141 ggcatgccat cgtcaaaggc cgcagcgcaa aacagatcgt ggaggctggc ccgacgctgg
     1201 gcaagggtgg cgactttatc gggctggatg cagccgtggc agacctcgtg cataccctca
     1261 ttgatgagtc atatcgcggt catccggatc tggtggtact ggttgcgccc gaccttgtgg
     1321 cacaggacaa ggtgacgctg cttaacgcca tcgacgcccc cacggaaaaa gtggccgcgc
     1381 agcttatcgg tcgccagatc gccgggctga cagcatacac cccgccgttc atgccggaag
     1441 gacgcctgat tgtcaccacg ctggcaaacc ttcacatcta cacgcagcgc ggcacccgtc
     1501 agcgccgtgc ggagtggaac gacgatcgca agcgttttga aaacaactat ctgcgcatgg
     1561 aaggttacgc cgtggagcac gacgaaattt acgcggccta cgacaagctg accatcaagg
     1621 gcgcagaagc ggcagaagag cccgcacctg gcggcgcata agcacagagg gcggcgcaat
     1681 gacaccctgc cagcgacacc gggcgaaaat ccgcacacag gaggcactcg aacgccggga
     1741 agcgctgacg gcctccccgg tcagcttcca cctgttacgc gcggagcttg atcgcgatgt
     1801 ggcacggctg cgcagtcttc ctgtccgcga agagcggctg gcgttcaagc gcgacattct
     1861 cctgccgcgc tggctgcccg tcgcggagcg ctatatcgct gacggaaagc gccacgcctg
     1921 cccggtactg gtgtactgca tcatctggct gttcgacacg ggcgacctga gtcgcgccct
     1981 tgactgggca gacattgcca tcagtgaagg ccaggccacg ccggacaact tccgcagcac
     2041 cctgcccgcc ttcgtggccg acacggtgtt gcagtgggcc accgacaaca gcgaggccgg
     2101 acacagcata gagccgtatt tttcgcgcac cttcaggaac gtcaccacca cgtggcggct
     2161 gcatgaggaa atccgcgcga agtggttcag atttgccggg ctgtatcttc tgcgcgacga
     2221 ggcaggcaaa ccgagagcca ccgcgattaa tgatactgcc gtactggaaa aggcagacgc
     2281 atacctggca caggcggcag cactgagccg taccgctggc gtaaaaagta tcagggcgcg
     2341 catcagggca cgcatcagcg cccttaatgc agcgtaaacg actacccgca gggccgggcg
     2401 ggcgcgggag gaggcatcag tgacggaaac tgtatggccg tggaatcccg gacagcccgc
     2461 ctttttattc atatgaggca cgtcatgagc gaaccgagct tcagcgtcag cggtaaaccg
     2521 ctgaaatcca caccgcagac ggtgacaaac ggcgagccgt tctggccgga ccttgatctg
     2581 gctgagcttc aggggtcacg cacgcttccg gctgatttac ccgacgacac ggcggccatg
     2641 gcgctgcttg ccgcagtggc cgaggtgaac accacgctgg cgccggttgt ggcgcactgg
     2701 aaggcgcagg ggcatgaacg cgccgcagac gtaccagggg ccaggctggg cgatgaaacc
     2761 cagctggtcg cacagtacaa aaaggccgtt tatgcccgtg ccaaggccga tcttatgggc
     2821 gagttcgcca ccattggtcg ccgtgaaacc tatccggggc aggaaagcga ggacacacgc
     2881 gccaccttac tggctgaagc ctcgctggtc atgcgtaaca tgctggggca cagacgcgcg
     2941 ggagtgcaca ttatatgaac cagctggaaa gcctgaccgc cttcattacc gaaaatctgc
     3001 cgcctgacgc catgcagttc ttcatgagca gtcaggaaga cgcgcagctt atccgcagtc
     3061 agaaggcgct gggtaacgac cagatccgca tgggcgtgat gacgtacacc ggcgtgattg
     3121 catgggataa cttcccgttc cgcaaatact caccggggct ggtttacgcg ctggtcatgg
     3181 cctggctgga agagcacgcg gacccgctga gggaggaatt ccgcccggag gatgtgaacg
     3241 tcgacccgca gtttaacgat gaggactcgt gccttctcga tatcacggtg ggactggctg
     3301 acccgctgat catccggatg caggaagacg gccccgttcc gtacaggggg aaacgctgga
     3361 cactggttaa ccctgaaatc tggacggcgg aaaccatgga tctgtttgtc agcaggagcc
     3421 gcgaatgata agcggccagc tgaacccgca gcagctggca gaagcgcagg cgatgcttca
     3481 gtcgctggat ctgccaccga aaaaacgccg tcgcctgtta tggcgtattg cccggcacgg
     3541 cattatcgcc gctgccagac gccatcagcg cgatcaggcc gcaccggacg gtacagcatg
     3601 ggcacccagg aaacgcggta aggggaaaat gttcagaaag ctgccgcgtc ttctggcggt
     3661 ccgtgagatg cccggacagg atgccgtaaa ggtttatttc cgtaacggca atcacaccag
     3721 tggcaggagc cgcatcagtg ccggtgccgt gggctgggtt caccacaacg gcgcagaaat
     3781 ccgggtcagt gctgacagct tcagcggtca ggaccagagc gggcagaccg ccacccgccg
     3841 acaggcaaaa cgactgcggg agctgggctt taaggtacgt cagaaggggc gctggcggaa
     3901 agcatccatg aaatacctgt taacgctttc gcgggcgcag gccgggttgc tgatcaccat
     3961 tatggaagat cagattatcg ccggaccacg ccagcagaag gcccggacat ggaccattac
     4021 ccttccgtcg cgcgtctttc tgggggtaac aaacgaagaa ttttcacaca tcctggcacg
     4081 ccagatgaac gcaatgggct acggcctgga cgttgacgca caggatatca gataaggagt
     4141 cacacagtga gctggccgaa tgtaaccgta agccatcaga accgtttcaa cggtgccacc
     4201 cgtgaagtgg agcgcacgct gctttttgtg ggctatggca aaaagaacac aggtaacacc
     4261 ctttccgtct cccctgagac cgacctggac gacgtgctgg gaccggatga aagcctgctt
     4321 aaatccacgc ttaccgccgc aatcgccaac ggtggccaga actggtttgc ctatgttcac
     4381 gttctgtctg aacccaaacc cccggcaccg gagggcggcg acgctaacgc cgcatgggtg
     4441 gacgcggtga aaaaagcgca gaccattgct tctgccgagg gcgtggtgat tgccattgac
     4501 atcacggcca aagatgccgt taaccgcgcc accgaaaccc gcgccctgct tcagtccgct
     4561 tatggccgtt ttgtctggtt catgctgtgc gtggccggac cgggcaagga tgaggcatgg
     4621 gcggcatacg tcacccgcat cagtgccata caggacggcg tggcagcacc tggcgtgatg
     4681 gtggttcccc gcttatgggg aaatgagccc ggcgtactgg cgggccgtct ctgtaatcct
     4741 tccgtcaccg ttgcggacag tccggcacgt gtggcaaccg gtgccctggt ggcgatgggt
     4801 aacgatgaga tcccgcagga cggcaagaaa cagccccttg agctggccac gctgcgctcg
     4861 ctggaaggca aacgcttcag cgtgcccatg tggtatcacg atttcgacgg gctctactgg
     4921 tcagacggac gcacccttga tgtggagggg ggagattacc aggtcatcga aaacgtgcgc
     4981 gtggtggata aagtctcccg ccgtgtacgt ctgcgcgcca ttgcccgcct tgcggatcgc
     5041 gcactcaaca gcacaccggg cagcattgcc gccagccaga catatttcag ccgcacgctg
     5101 cgtgaaatgg cgctcgccac ggagattaac ggcgtaccgt tccccggcga ggttaaaccg
     5161 ccacaggacg gggatatcac catcgtctgg accagcagta cacacgtgca gatttacatt
     5221 gtggtacgcc cctacgagag cgcaaaagcc attggcgtaa gtattgaact cgacacagca
     5281 ctggagatct gacatggccg gaaatgaacg tatcagcggc ggcgcgtttg atatcaacta
     5341 cgacgggatc atgatccacg tggaaagcgc caccgtcacc atcaccgaca acagcgccgt
     5401 ggcacagacg cgcggcgtgc cgaacggcta cacccgtggt tctgtttctg ccgatgtgga
     5461 ggtggaagtc gacgcgcaga actttaaaaa gtttggtgcc gtggcccgct ctgcggggtc
     5521 gtggcgtgcc attcctgaaa aggattttct cttttatgcc aacaacggca gcgaggagga
     5581 aaaaatcgag gtgttcggct gcgtccccat gttgtcggac gtggtgaaca tcagcacagg
     5641 tgaagccgga aagaccacga aaaaaatcaa gttcatggtc acaagcccgg actttatcag
     5701 cattgacggc gtgccctacc tgtcagcccg tgacacccgc gatctgaagg ggtgatgcca
     5761 tgccggacgg tcagacttca ctcctggcaa aactgattat catcggtgct gccatcgggc
     5821 tggggcgtct catggtgagc cgcgatcgca tcacagcccg tctgcttatc gggcgaatga
     5881 ttatgggctc tgccgtggca ccggttgccg ccattccgct gtttaaattt ccggacatgc
     5941 cggagctggt agtggtcggt ctggcgtgcg cgctgggcat tatgggcagc gcctttattg
     6001 aagaatgctt ttgccgtctg gcagaccgct acctgaagca gaaggaacgc agaaatgacc
     6061 cataagcctt acatttcgcc ttccctgagc ctgtatcacg cgcaggcgca tgacgttctg
     6121 cggatgctgc cggaaaacag tgtggacgcg ctcatcacag acccgccata cagttcaggt
     6181 gccacacaca aggcggggcg gacatgccag ggcagccacg ccaaatacat caacgatgaa
     6241 gatcttcacc gctttgaagg cttcgaaggc gagaacatgg acgcccgttc atgggaatac
     6301 tggacgcatc tgtggatgac acaggcatgg cgcacggtac gacccggtgg ttacgccctg
     6361 gtcttcaccg actggcggca gttgcccgcg ctgacggacg cattccaggc cgccggcttt
     6421 acctggcggg ggatcgtggc atggaacaag ggacgggcct caagaacacc gcataccggt
     6481 tattttcgtc accagtgcga atatgtggtc tggggcagta aaggcgcgct gggcaaatcc
     6541 ccttccgggc cgttcgacgg ctgcatcacg caccacacac gcccacagga gaaacagcac
     6601 ccgacaggta aaccggaatc gttgatggat gagcttgtgc gcgtgtgtga acccggaggg
     6661 ctgatcctcg atccgtttat ggggtccggc accacgggcg tggccgccct tcgccggggc
     6721 tgccgtttta cgggtattga actgaacgcc cactatttca gcgtggcaca ggcgcgcctt
     6781 acggggacgg aacagaggag tgactgatga aactgagcga aaaacaacaa ctgtttgccg
     6841 tcatgattgc cgatctcatc cactgggcac aggagcacgg ctatcgcctg accttcggcg
     6901 aggcataccg cacgccggag caggccgcac tgaatgcgaa aagcggaaaa ggcattcgca
     6961 acagccttca cacgctgcgc ctcgcggtgg acttcaacct gttcattaac ggcgaatacc
     7021 aggccgatac cgacgcctac aggccgctgg gggaatactg ggaatccatc ggcggcacat
     7081 ggggcggacg cttcagccgg gcagacggca atcatttcag ccttgagcac aacggggtga
     7141 aatgatgaaa cagctggcgg gctgcctggt gctcctcttt gccgccatgt gggcaggctg
     7201 gctggcccat gactggcatg acgccagggt ggcgcttgct gaacaggaag ccatggaagc
     7261 cacgcgacgt gcggtaacag aagccgggca gcagtccgcc cgcatactgg aaaaacggct
     7321 ggaggcgtta aagggcaatg aaattcacac tgaacgcgtt attcacacgg aaaccgtccg
     7381 cccggtattc cgcaatgtgt gcgctactga tgattacgtc cggctgttca acgcccgtgc
     7441 tgaagccgct gagcgtaccc tggcaggaaa accagctgac ggaatgcccg aagacactgc
     7501 cgagactggc aggcccgaca ggcgctgatt ttgatgcggc actgcgaacc ctcacgagcc
     7561 gctacaccga atgcgccgcc aggcataaca gcctggtgga aaccatacga aacacggaga
     7621 aactgcaatg accacgaaga acaccggcac catca
//
LOCUS       DAZKGF010000200         7481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZKGF010000200 DAZKGF010000000
VERSION     DAZKGF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.200"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..681)
                     /locus_tag="ACHOQV_004410"
     CDS             complement(<1..681)
                     /locus_tag="ACHOQV_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP9632685.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPAHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSG"
     gene            complement(692..1693)
                     /gene="ltaE"
                     /locus_tag="ACHOQV_004411"
     CDS             complement(692..1693)
                     /gene="ltaE"
                     /locus_tag="ACHOQV_004411"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFP9632686.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1730..3448)
                     /gene="poxB"
                     /locus_tag="ACHOQV_004412"
     CDS             complement(1730..3448)
                     /gene="poxB"
                     /locus_tag="ACHOQV_004412"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFP9632687.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3581..4549)
                     /gene="hcr"
                     /locus_tag="ACHOQV_004413"
     CDS             complement(3581..4549)
                     /gene="hcr"
                     /locus_tag="ACHOQV_004413"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFP9632688.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4561..6213)
                     /gene="hcp"
                     /locus_tag="ACHOQV_004414"
                     /gene_synonym="priS"
     CDS             complement(4561..6213)
                     /gene="hcp"
                     /locus_tag="ACHOQV_004414"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFP9632689.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6357..7256)
                     /gene="lysO"
                     /locus_tag="ACHOQV_004415"
     CDS             complement(6357..7256)
                     /gene="lysO"
                     /locus_tag="ACHOQV_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFP9632690.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHHQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         1811 a         2047 c         1954 g         1669 t
ORIGIN      
        1 accgctcacc gccataaaat gttcaaactg ttgctgataa ctgagcactt ctggtccggc
       61 ggcttcgaag atgcgatgtt cgctggccgg atgatctaac agcgccacca gatagtgcag
      121 caagttttcc agcgcgatgg gcgtggtgcg tgaacgaacc cagcgtggcg gcgttaacac
      181 cggcaggttg tagaccatat cgcgcatgac ttcgaacgcc gctgaacctg caccaacgat
      241 tattccggca cgtagttcgg tcaccgggac acccgcttca cgaagaatat ccgccgtagc
      301 ctgacgggcg cgcagatggt cagactgctc atgtgccggg gcctgcaacg aactgaggaa
      361 gattaattgc ttaacaggga cttcgcgtag cgcatcgcgg acgttgagag ccacctggcg
      421 ctcctgagcg ataaaatcgc cgccttcgcc catgctgtgc accagaaaat agaccgtatc
      481 gatatcctgc aacagggccg gaaggttatc cggccagctg agatcgactt tatggcaact
      541 gacgtttgcc agttgcagct ttgcaagcct gtcgacatga cgtgccgccg ccaggatctg
      601 atgcccttgc tggctgagag ttcgcaccag atgctgacca atgtagccac tggcaccgag
      661 aactaaaatg cgttgcggca cgtctctctc cttaacgcgc caggaacgca cgccagtggg
      721 cggcaacttc cgccagttgt tcacgcgaga cgtcaagatg ggtcaccagg cggacaatcg
      781 gcgaggcgtt aatcagcacg tttctcgctt tcatgtattc gcctaacgcg gcagcatttt
      841 cttccccgac gcgaacaaac agcatattgg tgtcctgacg catcacatcc gcgcctgctt
      901 cacgcaactg ctccgccatc caggcggcgt tatcgtggtc ttcctgcaaa cgtgcgacgt
      961 tatttttcag ggcatacatc ccggcggcag ccagaatgcc ggactgacgc atcccgccac
     1021 ctgtcatttt ccgccagcga atggcacgtt taatgtaatc acgattaccg acgagtaatg
     1081 aaccgaccgg cgtcccaaga cctttcgaca ggcaaatggt gaacgaatca caatattgcg
     1141 tgatctcttt cagttcgcag ccgtaagcca ccacggcatt aaagatgcgc gcaccgtcaa
     1201 catgcagcgc cagattgcgc tcgcgagtaa attcccatgc ttctttcagg tattcccgcg
     1261 gcaacacttt gccgttgtgg gtgttttcca gactgagtaa tttggtgcgg gcgaaatgga
     1321 tatcgtcggg tttgatcttc atcgccactt tatccagagg tagcgtgccg tcggcagccg
     1381 cgtcgatggg ttgcggctga atactgccca gcactgctgc gccaccggct tcaaacagat
     1441 agttatgcgc ggcctgaccg acgatatact cttcgccgcg ttcgcagtga ctgagcagag
     1501 cgaccaggtt ggcctgagtg ccggtaggca gaaaaatggc ggcttcttta ccggaaagct
     1561 ctgctgcata gtcctgcaga gcattaacgg tagggtcgtc tccgtaaacg tcgtccccaa
     1621 ccggggcggc catcatcgct tcgagcatgg cgcggctcgg tcgggtaacg gtatcactgc
     1681 gtaaatcaat catggcatgt ccttattatg acgggaaatg ccaccctttt taccttagcc
     1741 agttcgtttt cgccagctcg atcacttcat cgccgcgtcc gctgataatt gcgcgcagca
     1801 tatacaggct gaaacctttg gcctgttcga gtttgatctg cggtggaatg gctaactctt
     1861 ctttggcgac caccacatcc accaacaccg gaccgtcgat ggagaaggcg cgttgcaggg
     1921 cttcatcgac ttcagaggct ttttctacac gaatacccgt aatgccgcac gcttcggcaa
     1981 tgcgggcaaa gttggtgtcg tgcagctcag taccgtctgt caggtagcct ccggctttca
     2041 tctccatcgc cacaaagccc agcacgctgt tattaaagac gataattttc accggcagtt
     2101 tcatctgcac tactgagagg aaatcgccca tcaacatgct aaaaccgcca tcgccgcaca
     2161 tggcgaccac ctgacgttcc ggctcggttg cctgcgcacc cagcgcctgc ggcatggcgt
     2221 tagccatcga accgtggtta aacgaaccta acaggcgacg cttgccgttc atttttagat
     2281 aacgtgccgc ccacaccgtt ggcgtaccaa cgtcacaggt gaaaatagcg tcatcggcgg
     2341 caaaatgact aatttgctgc gccagatatt gcgggtgaat ggctttctcg ctcggtttag
     2401 ctaaatcgtc cagccctttg cgggcgtcgc ggtaatcttc cagcgcttta tccagaaact
     2461 tgcgatcggc tttttcttcc accaatggca gcaatgcacg cagagtcgac ttgatatcgc
     2521 cgaccagtgc catatccacc ttgctgtgag cgccgatgct ggctgggttg atatcaatct
     2581 gaataatttt ggcatcggtc gggtagaacg cacggtaggg aaactgagta ccgagcagca
     2641 ctaacgtgtc ggcgttcatc atggtatgga aacctgacga aaagccgatt aatcccgtca
     2701 ttccaacatc atatggatta tcgtattcga catgttcttt accgcgcagg gcatggacta
     2761 taggcgcttt aattttcccg gcaaactcaa ctaactcttt atgcgccccc gcacagccgc
     2821 tgccacacat cagggcgata ttgctggaat aacgcagcag ctgcgccagt ttgcgtaact
     2881 cttcttcttc cggtgttact accggctgtg gcgcatgata ccagtgggta gttgcccctt
     2941 ctggcgcagg ttttaacgcc acgtcgcccg gtaacacaac aacggaaacg ccacggttaa
     3001 gcaccgcttt gcgcatagca attgccagca cttgtgggat ctgctctggg ctggaaacca
     3061 gctcgcaata gtgactacat tcgcggaata gctcttgtgg gtgggtttcc tggaaatagc
     3121 cgctgccaat ttcgctggag ggaatatgag cggcaatcgc cagtaccgga acgtgattgc
     3181 ggtggcaatc gaacaggccg ttgattaagt gcaggttgcc gggaccgcac gatccggcac
     3241 agaccgccag ttctccgcta agttgtgctt cagcgccagc ggcaaatgcc gccacttctt
     3301 catgccgggt ggacatccac tcgatggtgc ccatgcgatt aagactgtca ctaagaccgt
     3361 tcagagagtc gcccgtgact ccccagatgc gtttcacgcc tgccgattcg agtgttttgg
     3421 cgatataagc tgcaaccgtt tgtttcatgg ttctccatct cctgaatgtg ataacggtaa
     3481 caagtttagt tcatctgacg gagggggaag ggatgggaga aaaaggaggc actaacggtt
     3541 aaatagcccg atgaaaggaa tatcatcggg catcaggcga ttatgcgaga accaaatccc
     3601 cctgcggatg gcaggagcag gccagtacat aaccttcagc gatttcggcg tcggtcagcg
     3661 tcattgtgct gctcaccgta tattcaccgg aaaccacttt cgtcttacag cagccgcaaa
     3721 cacctgcgcg gcaggcggcg acaaccggaa cgttattgct ttccagcgcc tccagtagcg
     3781 tggtgccaac cggggcgtaa aattctcgtg ccggttgcag tttggtgaat ttcagaccgc
     3841 tggtcgccgc ttccgctact ggggtgaaga atttctcttt aaagaaacgc gtcacgccga
     3901 gcgctttcac ttcctgctct acccaatcca tatatggagc agggccacag gtcatcacgg
     3961 tacgtgaggc taaatcaggg acacctgcca gcagttcgcg agtgagacga ccagcgataa
     4021 agccttcggt aacgttattt tccgccacca gcgttaccgg atagttacgc cactcatcgg
     4081 cgaaaatcac atcctgcggc gtacgcacgt tgtagatcac ctgcacatcg gcctgtggac
     4141 ggttcttcgc cagccagcga cgcatcgaca taatcggcgt aacgccgcag cctgccgcca
     4201 gcaacaggaa tttatcttct gctttatcat cgcaggtaaa ttcccccatc gcgtccgaaa
     4261 gccagagata atcaccgcgt tttacatcgc gcgtcagcca ctgggagccg acaccgtcat
     4321 caatccgccg cacagtcagg gtgatgtatt cactcacgcc tggcgtggag gaaatggtgt
     4381 aagcacgcag cgtttccgct gagttacgca cgctgaccag tgcatattgc ccggcgcgat
     4441 atgggtagta atcgtggcaa atcagggaaa tcgtccacac atccggcgtt tcttgcgtaa
     4501 tgtgatgaac ctgcatccgc cacgggcatt gattcgttgg catcgtcatc gacaaactcc
     4561 ttacgcgctc aacagttgct tcatgtcttc ttcaacagtg gtgatagaac gcaggccgaa
     4621 tttctcgttc agcaccgcca gcaggtctgg tgtcaggaaa ccaggtgcag tcgggccggt
     4681 gacgatattt ttcacaccca gagaaagcag cgtcagcaga atgacaatcg ctttctgttc
     4741 aaaccaggag agcaccagcg acagcggcag atcgttgact ccgcagccca gtttctctgc
     4801 cagcgttacc gccagaataa ttgctgagta agcatcgtta cattgaccag catctaccag
     4861 acgcggcaga ccttcgatat cgccaaactc aagtttatta aagcgatatt taccgcaagc
     4921 gagggtcagg atcaggcagt catccggcac gctggtggcg aaatcggtga aatagtgacg
     4981 ctcgccgcgt gcgccgtcac agccgccaag caggaagata tgacgcagtt tttcacggct
     5041 caccagatca atcagcgtat cggctgcgcc aagcagtgtc tggcgaccaa agcccacggt
     5101 gatcaggtgc ggaatttcgc tgtacgggaa gcccgccatc tgttgcgcct gggcaatgac
     5161 tgcagagaaa tcttcgccat ccagatgacg cacacccggc cagccaacga tgctgcgggt
     5221 ccagatacga tcgtcataag cgcctacggt tgggtcgatg atgcagttcg aggtcatcac
     5281 gatggggcct gggaaacgag cgaactccac ttgctgattc tgccagccgc tgccgtagtt
     5341 accgaccaga tgcttgaatt tacgcaactc cgggtagcca tgcgcaggca gcatttcgcc
     5401 gtgggtgtaa acattaacgc ccgtgccttc ggtctgttcc agcaggttgt agagatcttt
     5461 gagatcgtga ccggaaatca gaatgcattt acccgccgtc gctttgacgt tgacctgagt
     5521 tggcgtcggg tgaccgtatt taccggtttc gcctgcgtcc agaatgctca tcactttgaa
     5581 gttcatctgg ccgatttcca ttgaacactc aagaagcgcg ttcatatcgg caggccaggt
     5641 ccccagccac gccatgattt tatggtactg ggcataaata tcgttgtcgt attgaccgag
     5701 aacatgcgcg tgttccatat aggccgccgc acctttcagg ccatacaggc acagcagacg
     5761 caggccgaga atgttttcgc caatcgccgc tttatctttg ttaggggtaa attctgctgc
     5821 ctgacgttgc agctcgccga gatcgtcgct caccagttgc aggtcagcca tcgggttatc
     5881 gacgcgggcg ttggcatcta cagccaggca ttgtgctttc agcgcctcgc gcagggcaat
     5941 cgcttcacga gcgtagccga caatacgcgg agaatcgaag ttaacgttgg tcagggttga
     6001 gaaaaaggca cgtggcgcaa agctgtctac atcgtggttg ataatgccgt attcacgtgc
     6061 tttcactgcc caggccgaaa gcccttgcag cgccgcgatg agtaaatcct gaaggtcaga
     6121 ggtttctgcc gttttaccac acatcccctg cgcgtatgag cagccgtttc ctgccggagt
     6181 acggatagtt tgttcacatt gcacacaaaa catgatcaca ccttttaaag ttatatttaa
     6241 tatacatgtt taaggttaag acgcttaacg cggggataaa agagattttt aatgcaactt
     6301 taagggagat tgatttagcg caattttggc ggcagagatc taccgccaga gaggtattat
     6361 gcagagaaaa aggcgatgag aatcggcact aacagactaa gaataaaacc gtgaacaatc
     6421 gccgccggga ccatatccag cccgcctgta cgttgaagaa cgggcagggt gaaatccatc
     6481 gacgtcgcac cgcataagcc cagtgcagta gagcggctac ggcgaatcag cccagggatt
     6541 aacataatag caatcagctc acgggccaga tcattaaaaa acgccgcgct cccgattacc
     6601 ggaccaaaag attcggtcaa taaaataccg gaaagagaat accagccgaa accggaggcc
     6661 attgccagcg cggtattgat gggaagatca agaataaagg cgttaattaa accaccaatt
     6721 aatgaactgg caaccaccac cacggcgaca atcattcccc ggcgattaag gacaatctgc
     6781 tttaaggtca tgccattatt gcgcaactga ataccgacga ggaaaagtag cagaattaag
     6841 gtgtattcac tggcttcggt cgcgtgttgt aagaaagcca gtccactcag accaatggca
     6901 aaaccaatca ctactacgcc gcacagtttt agcgactcca gcgccatcgc aatacgcgac
     6961 gggagttttt cttgatgatg gtggttgcgc catggcaggc cgcgctccag ccacatcagg
     7021 gcggcaatat tacacagtaa aataacggta atactgacgg cagaataatg cagaatcgcc
     7081 aacaggttac tggcgaggtt atcgagaaac gccagactga tacccataaa aaagagaata
     7141 aggtaaacca tccagcttaa tagctgatta ataactctta atgcagcttt ttggcgaagc
     7201 ggaatgaggt aacccacaat caggggaacc agaatgatta acagcccaga aaacatgaaa
     7261 acccagtcct tgcaaagatg aagtcgaaat gcgcgatgac acactactga aagcggaagg
     7321 acgagtaaag ttgcaattaa aaggaaatgt tatgcatcag agggagattt ttaattctat
     7381 atgagtgttt agcattgttg cgctcctttc atgtgcaatg ccatataaag ccactggcgc
     7441 agggagcgcg cagggggcgg ccaatcgccg ccgccccctg c
//
LOCUS       DAZKGF010000201         7457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZKGF010000201 DAZKGF010000000
VERSION     DAZKGF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.201"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(84..171)
                     /locus_tag="ACHOQV_004416"
     tRNA            complement(84..171)
                     /locus_tag="ACHOQV_004416"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(135..137),aa:Ser,seq:gga)
     gene            404..1342
                     /gene="ghrA"
                     /locus_tag="ACHOQV_004417"
     CDS             404..1342
                     /gene="ghrA"
                     /locus_tag="ACHOQV_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFP9632691.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQARVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            1397..2134
                     /gene="ycdX"
                     /locus_tag="ACHOQV_004418"
     CDS             1397..2134
                     /gene="ycdX"
                     /locus_tag="ACHOQV_004418"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFP9632692.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            2158..2712
                     /gene="ycdY"
                     /locus_tag="ACHOQV_004419"
     CDS             2158..2712
                     /gene="ycdY"
                     /locus_tag="ACHOQV_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFP9632693.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            2814..3305
                     /gene="ycdZ"
                     /locus_tag="ACHOQV_004420"
     CDS             2814..3305
                     /gene="ycdZ"
                     /locus_tag="ACHOQV_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP9632694.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYAITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQQIK
                     NKA"
     gene            complement(3369..4202)
                     /gene="csgG"
                     /locus_tag="ACHOQV_004421"
     CDS             complement(3369..4202)
                     /gene="csgG"
                     /locus_tag="ACHOQV_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFP9632695.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(4229..4645)
                     /gene="csgF"
                     /locus_tag="ACHOQV_004422"
     CDS             complement(4229..4645)
                     /gene="csgF"
                     /locus_tag="ACHOQV_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFP9632696.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(4670..5059)
                     /gene="csgE"
                     /locus_tag="ACHOQV_004423"
     CDS             complement(4670..5059)
                     /gene="csgE"
                     /locus_tag="ACHOQV_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFP9632697.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(5064..5714)
                     /gene="csgD"
                     /locus_tag="ACHOQV_004424"
     CDS             complement(5064..5714)
                     /gene="csgD"
                     /locus_tag="ACHOQV_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFP9632698.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            6468..6923
                     /gene="csgB"
                     /locus_tag="ACHOQV_004425"
     CDS             6468..6923
                     /gene="csgB"
                     /locus_tag="ACHOQV_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFP9632699.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            6964..7419
                     /gene="csgA"
                     /locus_tag="ACHOQV_004426"
     CDS             6964..7419
                     /gene="csgA"
                     /locus_tag="ACHOQV_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFP9632700.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT         1973 a         1651 c         1802 g         2031 t
ORIGIN      
        1 tttcaaatgg catacccggt ggcatactgc ctggaaggat aaataaaaaa gtgcatgtta
       61 aacaataaca tgcgcttgta atctggcggt gaggggggga ttcgaacccc cgatacgttg
      121 ccgtatacac actttccagg cgtgctcctt cagccactcg gacacctcac caaattgtcg
      181 ttcctgtctt gctggaacgg gcgctaattt agggaaatca tgacctgagg tcaacaaact
      241 tttttaaaaa atcgctcgat tattcaaact tcaatcaata tgcagtttta ataagcgaaa
      301 tttgcttttt ttgccaccga ccacggattt gttatgctgc tggtctttgt agatcataac
      361 gataagtgcg aataaatttc gccaacactc ttcgggagtc agtatggata tcatctttta
      421 tcacccaacg ttcgataccc aatggtggat tgaggcactg cacaaagcta ttcctcaggc
      481 aagagtcaga gcatggaaaa gcggagacaa tgaatcagcc gattatgctt tagtctggca
      541 tcctcctgtt gaaatgctgg ctgggcgcga tcttaaagcg gtgtttgcac ttggggcagg
      601 tgttgattct attttgagca agctacaggc tcaccctgaa atgctgaagc cttctgttcc
      661 actttttcgc ctggaagata ccggtatggg cgagcaaatg caggaatatg ctgtcagtca
      721 ggtgctgcat tggtttcgac gttttgacga ttaccgcatc cagcaaaata gttcgcattg
      781 gcaaccgctg cctgaatatc atcgggaaga ttttaccatc ggcattttgg gtgcaggtgt
      841 actgggcagt aaagttgcac agagtctgca aacctggcgc ttcccgctgc gttgctggag
      901 tcgaacccgt aaatcgtggc ctggcgtgca aagctttgcc ggacgggaag aactctctgc
      961 atttctgagc caatgtcggg tattgattaa tttgttaccg aatacacctg aaaccgtcgg
     1021 cattattaat caacaattac tcgaaaaatt accggatggc gcgtatctcc tcaacctggc
     1081 gcgtggtgtt catgtcgtgg aagatgacct gctcgcggcg ctggatagcg gcaaagttaa
     1141 aggcgcaatg ctggatgttt ttaatcgtga acccttaccg ccggaaagtc cgctctggca
     1201 acatccacgc gtgacgataa caccacatgt cgccgcgatt acccgtcccg ctgaagctgt
     1261 ggattacatt tcgcgcacca ttgcccagct cgaaaaaggg gagagggtct gcgggcaagt
     1321 cgaccgcgca cgcggctact aataaagcat caggtttcct gctatccttg gcgggaattg
     1381 aatacaggag agagttatgt atcccgtcga ccttcatatg cataccgttg ccagcacgca
     1441 tgcatatagc acattgagtg attacattgc ccaggccaaa caaaagggca ttaaactttt
     1501 tgcgatcacc gatcatggcc cggatatgga agatgcgccg catcactggc acttcattaa
     1561 catgcgtatc tggccgcgag tggttgatgg ggttgggatc ctgcgcggca tcgaagctaa
     1621 cattaaaaat gttgacggtg aaattgactg cagtggcaaa atgtttgatt cgctggatct
     1681 gattattgcc ggttttcatg agccggtgtt tgcgccgcat gacaaagcga ccaatacgca
     1741 agcgatgatc gccactatcg ccagcggcaa tgtgcatata ataagccatc ccggaaatcc
     1801 gaaatatgaa atagatgtta aagccgttgc tgaagcagcc gcgaaacatc aggtggcgct
     1861 ggaaatcaat aattcctcat ttttacactc acgtaagggc agtgaagaca actgtcgggc
     1921 ggtagccgca gcggtacgtg acgctggagg ttgggtggca ttaggctcgg attctcacac
     1981 tgcgtttacc atgggggaat ttgaagagtg tcttaaaatc ctcgacgcgg tagattttcc
     2041 gccagagcgc attttgaatg tttctccgcg ccgcttactt aacttccttg aatctcgcgg
     2101 tatggcaccg attgcggaat ttgcagacct ttaattacta gtaatggaaa tatattaatg
     2161 aatgagtttt ctatcctctg tcgtgtgctg ggttcgctct attaccgcca accgcaagat
     2221 cctttactgg tgccgttgtt taccctgatt cgtgagggga aactggctgc gaactggcca
     2281 ctggagcagg atgagttact gacgcgttta cagaaaagct gtgatatgac gcaagtctct
     2341 gccgattaca atgcgttgtt tatcggcgat gaatgtgctg tgccgccata tcgtagcgca
     2401 tgggttgagg gcgcaacgga agcggaagtg cgcgcttttc tttccgaacg agggatgcca
     2461 ctagcggata cgccagccga tcacatcggc acattgctgc ttgcggcttc ctggctggaa
     2521 gatcagtcaa cggaagatga gagcgaagca ctggaaacac tgttcagtga gtatctgtta
     2581 ccctggtgtg gtgcgttcct tggcaaagtg gaggcccatg caactacgcc tttctggcgc
     2641 accatggcac cgctaacccg cgatgctatt agtgcaatgt gggacgagct ggaagaagat
     2701 tctgaagagt aaatgtgatc tgcatcactc ataactgcaa cgggtaattt gttgttgcat
     2761 aaaatgtgtg ctcgatctca ttcatggccg cgttttctgc tatcatgcgc ggcatgaaca
     2821 tacttctctc tattgcaatc acaacaggca ttctctccgg tatctgggga tgggtggctg
     2881 tttccctggg cttactaagc tgggcgggct tcctgggctg tacggcctac tttgcctgcc
     2941 cacaaggtgg gctgaaaggg ctggcgatct ccgctgcaac cctgcttagt ggcgtggtgt
     3001 gggcgatggt cattatttac ggtagtgcac tggcaccaca tctggaaata ctcggttatg
     3061 ccataaccgg tattgtcgcg tttctgatgt gtatccaggc caaacagctg ctgctttcat
     3121 ttgttccggg gacgtttata ggcgcatgcg cgacgtttgc cgggcagggt gactggaaac
     3181 tggtgttacc ttcgctggcg cttggactgg tatttggtta cgcaatgaaa aacagcggcc
     3241 tgtggttggc ggcgcgtagt gcaaagacgg ctcaccgtga gcagcaaatc aaaaataaag
     3301 cgtgaggggc actcacgctt tcgcttaaac cgtaaaatgt cggatgataa ttccggcatt
     3361 tttatctatc aggattccgg tggaaccgac atatggcggt atttcactag aatgtcattc
     3421 tgccgttctg ctttattttg caaatcccaa agaccgcggt cgataccatc attaatcagg
     3481 aaaatgacgc ctgtttcgat agccgacatc aggcacagca taacgggttc gttcgaggtg
     3541 taacctactt ccccttccag caagcgctgg tagtcaataa agcggaagac ccccgcctga
     3601 acttcatagg aaagtatcgt cttactggtg ttcaccgagg agaggatctc gccggtactc
     3661 acattgacga cgcgcaggtt cacggcaatc tgatcaagct ggtattgcgt gtctgcaccg
     3721 atcccaaaat atcttgcccc aaccccgccc gatttaacgt tgctttcata accgataatt
     3781 gaaccttcaa ccatgatatt tgccgccgtt aaagattgca gcgggattcg gttattaatg
     3841 gcaaccgtgc cgttttcttg tgccgcacga ataatcttgc gctcgttaag caggttttgt
     3901 aagccttggc gctccagcgg tataaaccag cgagaatctt tcaatgccgt gaccagcatt
     3961 gccgtggcgc tttgcggaac agcagtggag aagttacttg ccgggtaggg tttaaattgc
     4021 ccggtttcgt cctgaatgtt gtataccgaa acaaagatct tacccgtcgg cgctggcaga
     4081 tgggtcaaat ctttgtagct ctgagcacga ggcattaatg tcggtctggc ggcttcttta
     4141 ggcggggcgg ttaagcatcc gctcagtaac atgacggcaa ccaaaagaaa taagcgctgc
     4201 atgattattt tccttatgaa gctggggctt aaaaatcggt tgagttattt tgtaaacccg
     4261 aaacctggat ggtcgaggtt tgtccggttt tacgatctgt cacgttcaac tgcaattgac
     4321 catcgcggtt agcaatatca acaatataat cgttggtcac catgcggccc ggtttaccgg
     4381 tattaatatt cgacagtagc ccacccaaaa tttgtgactg gatggcctga gtaaagttat
     4441 ctaacgccga gggcgtttca ataccaaagt catcgttata gctcggatct ttataagagt
     4501 tttgggcctg agcgctattt aacaaaaaag cgccattatt tgggttacca ccaaagtttg
     4561 gattacggaa ctggaaagtc atggttccag cccaacttaa tggcgaaata agcatgagta
     4621 gaactactgc atgtttgaca cgcattgcag cctccgaaca atttattatt taaaattcat
     4681 catgcgccaa atcgcccgta cttaataacg cttggtttat ctggcgacga tttagtgctt
     4741 cttcagtttg aatcagtgca aagacgacag ttttctcgaa gtctcttttc aacggaaata
     4801 aaaaagtctg gaaaataacg tcctgattca ccgttattgt gatccagctt ccccatcgtg
     4861 cactgggcct ttcattaatc gttaagttac ccgtatagtc actttcccat ttatcactaa
     4921 aggctcggta aaaatcatgg ccaatagatg aaacagtatg gtcagttaac aatcccggga
     4981 cttctacctc aacggcgtga agattccctg cggcgaacag aaattctgcc gccacaatcc
     5041 agcgtaaata acgtttcatg gctttatcgc ctgaggttat cgtttgccca ggaaaccgct
     5101 tgtgtccggt tttttacggc tatcttcttg aaaagattat aaagatgcgt tttaaccgta
     5161 ttttcgctga tgaacaacga acgagcgatc tcgttattag acgcgccgat acgcagctta
     5221 ttcaggatct ctttttcccg atgagtaagg agggctgatt ccgtgctgtt ataacgatag
     5281 ttacctgaat gcgtaatcag gtaactggca agcttttgcg taaagtagca ttcgccgcgc
     5341 aggacgcctt gcaacccatt gacaacacgt tcttgatcct ccatggcata aaaaacgccg
     5401 ttgatatgag gccagttttc aatgtcgcgg tacgggtaat cttcaggcgt atttagcaac
     5461 aatattttga tattgttgtt tttcctgctc aaagtatcct gccaataatg gataagcttt
     5521 ttatccgctt ccatcatatc cagaagaata atagagcctg aagagatatc gtccagagaa
     5581 cgttgaatat tatgtaattt tcctgtaatt gccagcgatt gcttaaggtg ctgcaagaga
     5641 gctgtcgcct gcaaagaagg tttagtgatc aacaataatg tatgaccatg aatactatgg
     5701 acttcattaa acatgatgaa accccgcttt ttttattgat cgcacacctg acagctgcct
     5761 ctaaaataga agcaccagaa gtactgacag atgttgcact gctgtgtgta gtaataaatc
     5821 agccctaaat gggtaaaata taaaactaat ggattacatc tgatttcaat ctagccatta
     5881 caaatcttaa atcaagtgtt aaacatgtaa ctaaatgtaa ctcgttatat taaaatgtta
     5941 atcttaaggt tttattaagt ttagaaatga tagaaaagtt gtacatttgg tttttattgc
     6001 acaattttaa aaaatcatac aaatggtgct aacttactaa taatgcatat aaaaaatatt
     6061 tcggtgtagt cctttcgtca tgtaaaacgt tcttgttttt tctccacacc tccgtggaca
     6121 attttttact gcaaaaagac gaggtttgtc acggcttgtg cgcaagacat atcgcagcaa
     6181 tcagcgacgg gcaagaagaa tgattgtctg gtgatttttg atagcggaaa acggagattt
     6241 aaaagaaaac aaaatatttt ttgcgtagat aacagcgtat ttacgtgagt tttaatactt
     6301 tggtatgaac aaaaaaagaa aaatacaacg cgcgggtgag ttattaaaaa tatttccgca
     6361 gacatacttt ccatcgtaac gcagcgttaa caaaatacag gttgcgttaa caaccaagtt
     6421 gaaatgattt aatttcttaa atgtacgacc aggtccaggg tgacaacatg aaaaacaaat
     6481 tgttatttat gatgttaaca atactgggtg cgcctgggat tgcagccgca gcaggttatg
     6541 atttagctaa ttcagaatat aacttcgcgg taaatgaatt gagtaagtct tcatttaatc
     6601 aggcagccat aattggtcaa gctgggacta ataatagtgc tcagttacgg cagggaggct
     6661 caaaactttt ggcggttgtt gcgcaagaag gtagtagcaa ccgggcaaag attgaccaga
     6721 caggagatta taaccttgca tatattgatc aggcgggcag tgccaacgat gccagtattt
     6781 cgcaaggtgc ttatggtaat actgcgatga ttatccagaa aggttctggt aataaagcaa
     6841 atattacaca gtatggtact caaaaaacgg caattgtagt gcagagacag tcgcaaatgg
     6901 ctattcgcgt gacacaacgt taatttccat tcgactttta aatcaatccg atgggggttt
     6961 tacatgaaac ttttaaaagt agcagcaatt gcagcaatcg tattctctgg tagcgctctg
     7021 gcaggtgttg ttcctcagta cggcggcggc ggtaaccacg gtggtggcgg taataatagc
     7081 ggcccaaatt cagagctgaa catttaccag tacggtggcg gtaactctgc tcttgctctg
     7141 caagctgacg cccgtaactc tgatctgacc attacccagc acggcggcgg taacggcgca
     7201 gatgttggcc aaggttctga tgacagctca atcgatctga ctcagcgtgg tttcggcaac
     7261 agcgctactc ttgatcagtg gaatggtaaa gattctacta tgactgttaa acagttcggt
     7321 ggtggtaacg gtgctgctgt tgaccagact gcatctaact ccagcgtcaa cgtcactcag
     7381 gttggctttg gtaacaacgc gaccgctcat cagtactaat acatcctctg tattaaagaa
     7441 acagggcgca agccctg
//
LOCUS       DAZKGF010000202         7449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZKGF010000202 DAZKGF010000000
VERSION     DAZKGF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.202"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1438
                     /gene="citT"
                     /locus_tag="ACHOQV_004427"
     CDS             <1..1438
                     /gene="citT"
                     /locus_tag="ACHOQV_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFP9632701.1"
                     /translation="KLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPI
                     PATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVFGAF
                     IFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFP
                     VIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIA
                     GIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTR
                     REWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLV
                     NLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAH
                     TATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKD
                     YWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            1552..2358
                     /gene="rna"
                     /locus_tag="ACHOQV_004428"
     CDS             1552..2358
                     /gene="rna"
                     /locus_tag="ACHOQV_004428"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFP9632702.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETATKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISVKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            2588..2998
                     /gene="rnk"
                     /locus_tag="ACHOQV_004429"
     CDS             2588..2998
                     /gene="rnk"
                     /locus_tag="ACHOQV_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFP9632703.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            3141..3230
                     /gene="yldA"
                     /locus_tag="ACHOQV_004430"
     CDS             3141..3230
                     /gene="yldA"
                     /locus_tag="ACHOQV_004430"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFP9632704.1"
                     /translation="MSEALGITAFYIFIAAIIVAAVLYLEQRW"
     gene            3355..3783
                     /gene="uspG"
                     /locus_tag="ACHOQV_004431"
     CDS             3355..3783
                     /gene="uspG"
                     /locus_tag="ACHOQV_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFP9632705.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(3904..5469)
                     /gene="ahpF"
                     /locus_tag="ACHOQV_004432"
     CDS             complement(3904..5469)
                     /gene="ahpF"
                     /locus_tag="ACHOQV_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP9632706.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(5640..6203)
                     /gene="ahpC"
                     /locus_tag="ACHOQV_004433"
     CDS             complement(5640..6203)
                     /gene="ahpC"
                     /locus_tag="ACHOQV_004433"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP9632707.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            6575..7321
                     /gene="dsbG"
                     /locus_tag="ACHOQV_004434"
     CDS             6575..7321
                     /gene="dsbG"
                     /locus_tag="ACHOQV_004434"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFP9632708.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKMLNIIMGNK"
BASE COUNT         1808 a         1864 c         1819 g         1958 t
ORIGIN      
        1 gaaattattg gcgcccctgg tggtgatggg tgtcatgttt cttatccctg tacctgacgg
       61 tatgccgccg caggcgtggc attactttgc agtgtttgtg gcaatgattg tcggcatgat
      121 cctcgagcca attccggcaa cggcgatcag ttttattgcg gttactattt gcgttattgg
      181 cagtaattac ctgctctttg atgccaaaga attagctgac ccagcgttta atgcgcaaaa
      241 acaggcgctg aaatggggtc tggctggttt ttccagcacc acggtatggc tggtatttgg
      301 cgcatttatt tttgcattag ggtatgaagt ttccgggtta ggccgtcgta ttgccctttt
      361 cctggtgaaa ttcatgggca aacgcacgct gacgttgggt tatgcgattg tcattatcga
      421 cattctgctg gcaccgttta caccgtccaa caccgcgcgt accgggggta cggtttttcc
      481 ggtcattaaa aacctgccgc cgttgtttaa atcattcccg aacgatccgt ccgcgcgtcg
      541 tattggcggc tatttgatgt ggatgatggt cattagtacc agtctgagtt cgtccatgtt
      601 tgtcaccggt gcggcaccaa acgtgctggg tctggagttc gtcagcaaaa ttgccggtat
      661 ccagattagc tggttgcagt ggttcctctg cttcctgccg gttggggtta tcttgcttat
      721 cattgcgccg tggctttcct acgtgctgta caaaccggaa atcacacaca gtgaagaagt
      781 ggcaacctgg gcgggtgatg aactaaaaac catgggtgcg ctgacacgca gagagtggac
      841 gctgattggc cttgtattgc tcagcttagg tttgtgggta tttggcagtg aagtcattaa
      901 tgctactgcg gttggtctgc tggcagtttc gctaatgctg gccctgcacg ttgtgccgtg
      961 gaaagacatt acccgctata acagcgcatg gaacacgctg gtcaacctgg caactctggt
     1021 tgtgatggct aacggcctga ctcgttctgg ttttattgac tggttcgcca ataccatgag
     1081 tacgcacctg gaaggattct caccaaacgc aacggtgatt gtactggttc tggtgttcta
     1141 ctttgcacac tacctgtttg ccagcctgtc tgcgcacacc gcaaccatgc tgccggttat
     1201 tctggccgtc ggtaaaggta ttccgggcgt accaatggaa caactgtgta tcctgctggt
     1261 gctatctatc ggtatcatgg gctgtctgac gccgtatgca accggtcctg gggtgattat
     1321 ttacggctgt ggctatgtga aatcaaaaga ttactggcgt cttggcgcaa tcttcggggt
     1381 gatttacatc tctatgttgc tgttggttgg ctggccgatt ctcgccatgt ggaactaata
     1441 cgttcgccaa acgctgaata aatcatcacg cccgccaggt aaacccacct ggcgggcttt
     1501 tttatgatta aatagataat attcagtgta tgtatgagtt ccacacccat tatgaaagca
     1561 ttctggcgta acgccgcgtt gctcgcggtt tctctgcttc ccttctcttc tgccaacgcc
     1621 gtggcgttgc aggcaaaaca gtatggcgat tttgatcgct atgtcctggc cctctcctgg
     1681 caaaccggat tttgccagag tcaacacgat cgaaatcgta acgaacgaga tgaatgtcgc
     1741 ctgcaaaccg aaacgaccaa caaagctgat tttctgaccg tacatggtct gtggccagga
     1801 ttgcctaaat cggttgctgc ccgtggtgtt gatgaacgcc gctggatgcg cttcggttgc
     1861 gctactcgcc caatcccgaa tctaccagaa gcgcgcgcca gccgaatgtg ttcatcgccg
     1921 gaaaccgggt tatcactgga aacggccact aaactaagtg aagtcatgcc aggagctggc
     1981 ggacgttcct gcctggaacg ctacgaatat gccaaacacg gtgcctgctt tggttttgat
     2041 ccagacgcat acttcggcac gatggtacgc ctgaatcaag aaattaaaga aagcgaagcc
     2101 ggaaaattcc ttgcggataa ctacggtaaa acagtgagcc gccgtgactt tgatgccgcc
     2161 tttgccaaaa gctggggaaa agagaacgtg aaagcagtta agctaacgtg ccagggtaac
     2221 cctgcgtatt tgactgaaat tcagatctcg gtcaaagctg acgccatcaa cgctccgctt
     2281 tctgccaact cattcttgcc acaaccacac ccaggcaact gtggcaaaac ctttgtgatt
     2341 gataaagcag gttattaatc ctctgattta caaaacacgc taattcgtct tccataaacc
     2401 atgacgatta agatttatac ataatgcgct acattgagga attccctgaa tgtgacgcaa
     2461 atcacttcaa agagtgagca acagtcagta tcatcatgtg agttaaaccc tcgccgcctg
     2521 acggtgaggg ttttcttttg ggtatgttta tctgcgacac tcgcagtacc gacgacatgg
     2581 agtaaaaatg tccagaccaa ctatcatcat taacgacctg gatgccgaac gcatcgatat
     2641 tctgctggag caacccgcct atgctggttt gccaatcgcc gacgcgttaa acgcagagtt
     2701 ggatcgcgcc caaatgtgtt cgccagaaga gatgccacac gacgtggtga caatgaacag
     2761 ccgggttaaa ttccgcaatc ttagcgatgg cgaagtgcgt gtgcgcacgc tggtgtatcc
     2821 ggcaaaaatg accgacagca atactcagct ttccgttatg gctccggtag gtgccgcact
     2881 gctggggctg cgcgttggcg attccattca ctgggaactt ccgggcggcg ttgcaaccca
     2941 ccttgaagtg ctggaactcg aataccagcc agaagctgct ggcgactacc tgctttaatc
     3001 ccatctgcac aagcgataac tcgctttgcc cagaggatgc actcgcatcc tctttccgct
     3061 caaatatcaa ccagatttat cattttgctc ttaagcgcag cattctaatc tgaactggct
     3121 attcatctgc ggaacctcat atgagcgaag ctctcggtat cactgcattt tatatattca
     3181 tagcagcaat tattgttgct gcagtgctat accttgaaca acgctggtag cgcctggttt
     3241 ttatttattt tccagctcaa tagcaaattc agatcaaatc agcctcgcaa attatagatt
     3301 cgtaaccaat aatgtgctgt tatgtataca tgcaaaaaca aggagagaat gcttatgtac
     3361 aaaacaatca tcatgccagt ggatgttttc gaaatggaat tgagcgacaa agcgattcgc
     3421 cacgctgaat tcctcgccca ggatgacgga gttattcatc tacttcacgt actaccgggg
     3481 tcagccagcc tgagcctgca ccgttttgcc gctgatgtac gtcgttttga agagcatctg
     3541 caacatgaag cagaagaacg tctgcaaacg atggtcagcc acttcaccat cgatccttcc
     3601 cgcattaagc aacatgtccg ttttggtagt gtgcgggatg aagttaacga gttagcgaaa
     3661 gaacttgatg cagatgtagt agtgattggt tcgcgtaacc catcaatttc aactcatctg
     3721 ttgggttcta acgcctcgag cgtaatccgc cacgccaatt tgccggtgct ggttgtgcgt
     3781 taattgccag aataagtctc ccgccctgcc cggagccatc cctgcagggc tttttaatgc
     3841 aataaaaaag ccgccaggtt tgaccccggc ggctaagcaa ttacaggtga atcttacttc
     3901 ttcttatgca gttttggtgc gaatcaggta gtcaaaagca ctcagagagg ctttggcacc
     3961 ttcgccagtg gcgatgatga tctgcttgta cggaaccgtc gtacagtcac ccgctgcgaa
     4021 cacgcctttg acgttggttt cgcatttcgc atcaatgata atctcgccca tgcgattacg
     4081 ttcgactgcg ccttccagcc agttggtgtt cggcagcaga ccaatctgca cgaaaatacc
     4141 ggccagttcg atgttgtgaa tatcgccgct gacacgatct cgatattcca gacctacgac
     4201 tttgctaccg tcgcctttca cttccgtggt ttgcgcattc agaataatgt cgacgttttt
     4261 caggctgcgc agtttgtcct gcagaacctg gtcggctttc atttctggcg caaattccag
     4321 cagcgttacg tgctcaacga tacccgccag gtcaattgcc gcttccacgc cggagttacc
     4381 gccgccgata accgctacgc gtttaccttt aaacagcggg ccgtcgcagt gcgggcagta
     4441 ggtcacgcct ttggtgcgat actgatcttc gcccggaacg ttcatgttgc gccattttgc
     4501 accggtcgcc acgataatgc tgcgtgcttt cagtaccgcg ccagaagctg tttcaatctg
     4561 atgcaggcca ccttcaaccg ctgccgggat cagtttgctg gcgctctggc tgtcgatcac
     4621 atcaacgtcg tattcatcaa cgtgaacttt cagcgctcct gccagtttct ggccttcggt
     4681 cttcggtaca gaaatgtagt tttcgatatc aacggtatcg aggatctgac caccaaaacg
     4741 ttcgcccatc agaccggtac ggatgccttt acgtgcggag taaattgctg ccgctgcacc
     4801 cgccgggccg gaaccgacga ttaatacgtc ataagcatca cgcttgttca gctcttctgc
     4861 cgcacgtttt tccgcaccag tatcaatttt ggcaacgatt tcagtcaacg tcatgcggcc
     4921 ctgaccaaac tctttcccgt ttacgaacac tgccggaacg cccatcacgt tgcgatcggt
     4981 gatttcgttc tggaaggtgc cgccgtcaat tgcagtgtgc ttgatgcgcg ggttcagtac
     5041 gctcatcagg ttcagcgcct gcaccacgtc cgggcagttg tggcaagaga gcgagtaata
     5101 ggtttcgaat tcaaaatcac cgtcaatatg gcgaatctgc tccagcagag actgcgcttc
     5161 tttcgacgga tgaccaccgg tccacagcaa cgccagtacc agcgaggtga actcgtggcc
     5221 cagcggggag cctgcaaaac gtggcccctg gttagaacct gggttggtga tcaggaaaga
     5281 cggcttacgc accggcaagc tgttatcttc tttaaaggtg actttgtctg acagttctgc
     5341 gatttcagcc aacagttcct tgatttctgc cgatttagcg ctgtcatcca gcgtggcaat
     5401 taactcaaca ggcttggtca atttctcaag gtaagccttg agttgagttt tcatatttgt
     5461 gtcgagcatg tttatctcct gggctttaaa catcatcatg caagcggcct ctgcggctac
     5521 ctggatgcag cttgcatcat ggtgcgggga agaaaatcgg gtgccgcagc acccgaagaa
     5581 ttaggtgaat ttccgaagta tttcgcgccg gagttagtag cgcgaaagac aaaggaaatt
     5641 tagattttac caaccaggtc cagagacgga gccagagttg cttcaccttc tttccattta
     5701 gccgggcaaa cttcacctgg gtgagaagct acgtactgtg ctgctttgat tttacgcagc
     5761 aggtcagacg cgtcacggcc aatgccttca gcggtaactt cgattgcctg gatgataccc
     5821 tgcgggtcaa caacgaaggt cgcacggtca gccagacctt catcttcacg catgttgtcg
     5881 aagttacggg tcagggcgcc agtcgggtcg ccgatcatcg catatttgat tttagcgatg
     5941 gtttcagagc tgctgtgcca tgctttgtgg gtgaagtgag tatcggtaga tactgcgtat
     6001 acgtctacgc ccagtttctg caattcttcg taatggtcag caacgtcacc cagttcggtc
     6061 gggcatacga aagtaaagtc agccgggtag aagaagaaga cgctccagcg gccttcggta
     6121 tctttttcgg tgatttcgat gaattcgccg tttttgaatg cctggttttt aaaaggttta
     6181 attttggtgt taatcaagga catctatact tcctccgtgt tttcgatgag atgtaaggta
     6241 accaattttt gccgattcgg ctaatgcgtt tccattatca aatcgataag ttttacctta
     6301 caaccttcgt aagacaactt tgtgaacttt gcaccgccag cggcgataaa aagtaaaatg
     6361 gccccctttc caggtgaccg tgtaatctga actacccttt ggtatcttca gcgcctgcct
     6421 gcaggctgag gtgttgccct tacaaatgca acaacgacat ggattacaac accctcataa
     6481 acaaagggca atcacctgat ctaagctctt acctatgaca gtgataggtt atgcctttta
     6541 ctcgactttt gcactgactg aaaaggacaa attaatgtta aaaaagatac ttttactggc
     6601 tctgcttcct gcaatcgcct tcgcagagga acttcctgcc ccagtaaaag cgattgaaaa
     6661 acagggtatt acaatcatca aaacgttcga tgcccctgga ggaatgaaag gttatctcgg
     6721 taagtatcag gatatgggcg tcaccatcta cctgactcca gatggtaagc acgctatctc
     6781 tggttacatg tacaacgaaa aaggtgaaaa cctgagtaac acgcttatcg aaaaagaaat
     6841 ttacgcaccc gccggacgtg aaatgtggca acggatggaa caatcccact ggctcctcga
     6901 cggtaaaaaa gatgcgccgg tcattgtcta cgtcttcgcc gatccgttct gcccatattg
     6961 taaacaattc tggcagcagg ctcgtccgtg ggtagattct ggcaaagtgc aattaagaac
     7021 attgttggtt ggggttatca agccagaaag cccggcgaca gcagcggcaa ttcttgcctc
     7081 caaagatccc gcaaaaacct ggcaacaata tgaagcctct ggtggcaagc ttaagctaag
     7141 cgtgcctgca aacgtaagca cagagcaaat gaaagtgtta agcgacaacg agaaactcat
     7201 ggacgatctg ggggcaaatg tcacaccagc tatctattac atgagtaagg aaaataccct
     7261 gcaacaagcc gtcggattac ccgatcagaa aatgcttaat atcattatgg ggaataaata
     7321 aaagataatt catggatggt tgaaatatat ccttgctttt caaccatcct aaaaacgcat
     7381 gaattgctcg taacccacct gacagctatg ctattttaga aaatcttaat ccaaagttaa
     7441 gcagaaaaa
//
LOCUS       DAZKGF010000203         7424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZKGF010000203 DAZKGF010000000
VERSION     DAZKGF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.203"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..850
                     /gene="gatY"
                     /locus_tag="ACHOQV_004435"
     CDS             2..850
                     /gene="gatY"
                     /locus_tag="ACHOQV_004435"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFP9632709.1"
                     /translation="MVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVI
                     IAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMID
                     ASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQARE
                     FAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDIQQ
                     TIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADC
                     GCEGRA"
     gene            879..2141
                     /gene="gatZ"
                     /locus_tag="ACHOQV_004436"
     CDS             879..2141
                     /gene="gatZ"
                     /locus_tag="ACHOQV_004436"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFP9632710.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMFDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGMEIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2151..2603
                     /gene="gatA"
                     /locus_tag="ACHOQV_004437"
     CDS             2151..2603
                     /gene="gatA"
                     /locus_tag="ACHOQV_004437"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFP9632711.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPEIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2634..2918
                     /gene="gatB"
                     /locus_tag="ACHOQV_004438"
     CDS             2634..2918
                     /gene="gatB"
                     /locus_tag="ACHOQV_004438"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFP9632712.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            2922..4277
                     /locus_tag="ACHOQV_004439"
     CDS             2922..4277
                     /locus_tag="ACHOQV_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP9632713.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            4325..5365
                     /gene="gatD"
                     /locus_tag="ACHOQV_004440"
     CDS             4325..5365
                     /gene="gatD"
                     /locus_tag="ACHOQV_004440"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9632714.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDVVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            5471..6244
                     /locus_tag="ACHOQV_004441"
     CDS             5471..6244
                     /locus_tag="ACHOQV_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9632715.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6326..7225)
                     /gene="yegS"
                     /locus_tag="ACHOQV_004442"
     CDS             complement(6326..7225)
                     /gene="yegS"
                     /locus_tag="ACHOQV_004442"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFP9632716.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1859 a         1656 c         1951 g         1958 t
ORIGIN      
        1 cgtggtatcg acaaagcaga tgctgaacaa cgcacagcgc ggcggttatg cggttccggc
       61 attcaatatt cacaatctcg aaacgatgca agtggtggta gaaaccgctg ccaacctgca
      121 tgcgccggtc atcatcgccg gaacgcctgg cacatttact catgccggta cagaaaatct
      181 gttggcgctg gtcagcgcga tggcgaagca atatcaccat ccactggcaa ttcatctcga
      241 ccatcacacg aaatttgacg atatcgctca gaaggttcgt tctggcgtgc gctcagtcat
      301 gattgacgcc tcgcatttgc cttttgcgca aaatatttca cgggtcaaag aggtggtgga
      361 tttttgccat cgctttgatg tcagcgtcga agcggagctg gggcaacttg gcggccagga
      421 agatgatgtg caagtcaatg aagccgatgc gttgtacacc aaccctgttc aggcgcgtga
      481 atttgccgag gcaaccggaa ttgattccct ggcggtcgcc atcggcacgg ctcacggtat
      541 gtatgccagc gcaccggcgc ttgatttttc tcgactggag aacattcgcc agtgggtgaa
      601 cttaccgctg gtgctgcatg gcgcgtcagg attatcaact aaggatattc agcaaaccat
      661 caaactgggg atatgcaaaa tcaacgttgc aacagagcta aaaaatgcct tctcgcaggc
      721 gttaaaaaat tacctgacag agcaccccga agcgaccgat ccccgggatt atttgcagtc
      781 ggctaaatcc gcaatgcgcg atgtggtgag caaagtgatt gccgattgtg gctgcgaggg
      841 cagggcataa cgcacctgcc atttaacaag gaaaaaacat gaaaacgtta attgcccggc
      901 ataaagctgg tgaacatatt ggcatatgtt cagtctgttc tgcacatccg ttggttatcg
      961 aagcggcgct ggcatttgat cgcaacagca cgcgtaaagt gctgattgaa gcaacgtcaa
     1021 accaggtcaa tcaatttggc ggttataccg gaatgacacc ggcagacttt cgcgaatttg
     1081 tttttgcgat tgccgataaa gtcggatttg ctcgtgagcg tattattctc ggtggcgacc
     1141 atctgggacc aaactgctgg cagcaagaaa atgtggatgc ggcgatggaa aaatccgtcg
     1201 aactggtaaa ggcatatgtt cgtgccggct tcagtaaaat tcatcttgat gcgtcaatgt
     1261 cttgcgcggg ggatcctata ccgttagccc cagaaacggt tgcggaacga gctgctgtgc
     1321 tttgctttgc tgcggaaagt gtggcgacag attgccagcg tgagcaactg agctatgtca
     1381 ttggcaccga agttccggtt ccgggcggtg aggccagcgc cattcagtca gtacatatca
     1441 cccaggttga agatgccgcc aatactttac gcacgcatca aaaagcgttt attgcccgcg
     1501 gtctggcaga ggcgttaaca cgcgtgattg ccatcgtggt gcagccgggt gtggaatttg
     1561 accatagcaa tattattcat tatcaggcgc aagaagctca ggcgctggcg caatggatag
     1621 aaaaaaccaa aatggtttat gaagcgcatt ccaccgatta ccagactcag acggcctatc
     1681 gggaattagt tcgcgatcat tttgcaattt tgaaagtcgg tccggcatta acctttgctt
     1741 tacgtgaggc gatatttgcg ctggcgcaaa ttgagcagga acttatcgcc ccagaaaatc
     1801 gcagcggttg cctggcggta attgaagaag tgatgtttga cgaaccgcaa tactggaaaa
     1861 aatattatcg tacgggtttt aacgattcat tactggatat tcgttacagc ctgtcggatc
     1921 gtattcgtta ttactggccg catagccgga ttaaaaatag cgtcgaaacg atgatggtga
     1981 atctgcaagg catggaaatc cctctgggca tgattagtca gtatcttcct aaacaatttg
     2041 aacgcattca gtccggggaa ttatcagcaa taccgcatca gttgattatg gataaaattt
     2101 atgatgtttt gcgcgcctat cgctacggct gtgcggaata aggacggtat atgactaacc
     2161 tgtttgttcg tagcggaatt tcttttgtcg atcgtagcga agttttaacc catatcggta
     2221 atgagatgct cgcgaaaggt gtggttcatg atacctggcc acaggcatta attgccagag
     2281 aagcagaatt ccctaccggg ataatgcttg agcagcacgc cattgcaata ccgcattgtg
     2341 aggcgattca tgctaagtcg tcagccattt atctgttaag gccaacaaat aaagttcatt
     2401 ttcagcaagc ggatgatgat aacgacgtgg cggtatcgtt ggtgattgcg ttaattgtgg
     2461 aaaatccgca gcagcaattg aaacttttac gctgtttatt tggcaagtta caacagcccg
     2521 agatcgtcga gacactaatc actcttcctg aaacccagtt aaaggaatac ttcacaaagt
     2581 atgttttaga ttcagacgaa taaatccctc tgtaacaata aaaaggacta tttatgaaac
     2641 gcaagattat tgtcgcttgc ggaggcgcgg ttgcgacctc tacgatggcg gcggaagaaa
     2701 ttaaagagtt gtgtcagagt cataatattc ctgttgaatt aatccagtgt cgggttaatg
     2761 aaatagaaac ctatatggat ggcgtgcatt tgatatgcac cactgccagg gtggatcgaa
     2821 gttttggcga tattccgtta gttcacggca tgccttttgt ttctggtgtc ggtatcgaag
     2881 cattacaaaa taaaattctg actatcttac aggggtgacc tatgttttca gaagtcatgc
     2941 gttatattct cgacctcggc cctacggtga tgctgccgat tgtcatcatt attttttcta
     3001 aaatattagg catgaaggca ggcgattgct ttaaagcagg tctgcatatc gggattggct
     3061 ttgttggtat tggccttgtg attggcttaa tgctggattc cattggcccg gcggcgaaag
     3121 cgatggcgga aaatttcgac ctgaatctgc atgtggtcga tgtcggctgg ccgggctctt
     3181 cacccatgac ctgggcgtcg caaattgcgc tggtggcgat tccgattgcg attctggtta
     3241 acgtggcgat gttactgacc cgtatgacgc gggtggtaaa tgttgatatc tggaatatct
     3301 ggcatatgac cttcaccggc gcgttgctgc atctggcaac cggttcatgg atgataggga
     3361 tggcgggtgt ggtaattcac gcggcgtttg tttataagct cggcgactgg tttgcccgcg
     3421 atacccgaaa tttctttgag ctggaaggca ttgctattcc gcacggtacg tcggcgtata
     3481 tggggccgat tgcggtgctg gtcgatgcta tcatcgagaa aatcccaggc gttaaccgaa
     3541 ttaaatttag cgccgatgat attcagcgca aatttggccc ttttggcgaa ccggtcaccg
     3601 tgggttttgt gatggggctg attatcggca tcctcgcggg ttacgatgtc aaaggtgtat
     3661 tgcagctggc ggtaaaaacg gcggcagtta tgctgctgat gccacgggtg attaaaccca
     3721 tcatggatgg tttaacgccc atcgctaagc aggcccgtag tcgtttacag gcgaagttcg
     3781 gcggtcagga gttcctgatt ggccttgatc cggcgttgct gctgggacat acggcggtgg
     3841 tatcggcaag cctgattttt attccactca ccattttaat tgctgtttgt gtgccgggta
     3901 atcaggtgct gccgtttggc gaccttgcca ccatcggctt ctttgtggcg atggcggtcg
     3961 cggtgcatcg tggaaatctg ttccgcacct taatctcggg tgtcatcatt atgagcatca
     4021 ccctgtggat cgcgacgcaa actattggtt tgcacaccca actggcggct aatgctgggg
     4081 cgttaaaagc cgggggcatg gtggcttcaa tggatcaggg cggttctccc attacctggt
     4141 tactgattca ggttttctcc ccgcaaaata ttcccggttt cattattatc ggtgccattt
     4201 atcttaccgg tattttcatg acctggcgta gagcgcgtgg ctttattaaa caagagaaag
     4261 ccgttctcgc agaataattt ttacctgagg gggaggttat cccccttaat aatcaggagt
     4321 ttttatgaaa tcagtggtga atgatactga tggtatcgtg cgcgttgcag aaagcgtcat
     4381 tcctgaaatt aaacatcagg atgaggtgcg ggtaaaaatt gccagctcgg gcttatgtgg
     4441 ttccgattta cccaggatat ttaaaaatgg tgcacattat tatccaataa cgttaggtca
     4501 tgaatttagc ggctatattg atgcggtggg atccggtgtt gatgatttac accctggcga
     4561 tgtggttgcc tgtgtgccgt tattaccctg ttttacttgt ccagagtgtt tgaaagggtt
     4621 ttattcccag tgcgcaaaat atgattttat tggctcgcgg cgtgatggtg gatttgccga
     4681 atatattgtc gttaagcgaa acaatgtctt tgcactacct gcggatatgc ctattgagga
     4741 tggcgctttt attgaaccga ttaccgttgg cctgcatgcg ttccatttag cgcaaggatg
     4801 tgagaataaa aacgttatta ttattggtgc cggaaccatt ggcctgctgg caattcagtg
     4861 cgctgtcgcg ctgggagcaa agagtgtgac ggctatcgac attagctcag aaaaactggc
     4921 actggcaaaa tctttcggag cgatgcaaac atttaacagc cgtgaaatga gcgcgccgca
     4981 aatacagggc gttttacgcg agctgcgctt taatcagctt atcctcgaga cggctggcgt
     5041 cccgcaaacc gtcgaactgg cggtagagat tgccggtcct catgcccaac tggcgctggt
     5101 gggcacgttg catcaggatc tgcatttaac atctgcaacg tttggcaaaa tattgcgtaa
     5161 agagctgacg gttatcggca gttggatgaa ctactccagc ccttggccgg ggcaggagtg
     5221 ggaaacggcg agccggttgc tgacagaacg taagttaagc ctggagccat taatcgctca
     5281 ccgtggaagc tttgaaagct tcgcccaggc ggtgcgtgac atcgctcgta atgccatgcc
     5341 gggcaaagtg ttgctcattc cctgaaaccg cgggccagcg tgatgcaggc ccggtattgt
     5401 gcaaaacaga tcattcacca atggtccccc ttcgtttaca ctagccacaa ttgaatatgg
     5461 gtaaatgacg atgaattcat tcgagcgaag gaataagatc atccaattag tgaatgaaca
     5521 gggaaccgtg cttgttcagg atctggcggg agtatttgct gcctcggaag cgacaatccg
     5581 tgccgatttg cgctttctcg aacaaaaagg cgtggttacg cgctttcatg gcggtgcggc
     5641 gaaaataatg tccggtaata gcgaaaccga gacccaggaa gtcgggttta aagagcgatt
     5701 tcagctcgcc agcgcgccga aaaacagaat agcgcaggcg gcagtcaaaa tgatccacga
     5761 agggatgacc gttattctcg acagcggaag tacgacaatg cttatcgccg aaggattaat
     5821 gaccgccaaa aatatcacgg tgattaccaa cagtctcccg gctgcgtttg ccctttccga
     5881 aaataaagac atcactttgg tggtttgtgg tggtacggtg cgccataaaa cgcgctcgat
     5941 gcatggttcc attgctgagc gttcattgca ggatattaat gccgatttaa tgtttgtcgg
     6001 tgctgatggt attgatgcgg ttaatggtat tacgaccttt aatgaaggtt attccgttag
     6061 cggtgcgatg gtgacagcgg cgaataaagt gattgccgtt ctcgattcat cgaaatttaa
     6121 tcgtcgcggt tttaatcagg tactgccaat cgaaaaaatt gacattatta ttaccgatga
     6181 tgctgtgtct gaggtggata aactggcatt gcagaagaca cgggtaaaat taattacggt
     6241 gtagtattgt tctgtatgcc ggataaggtg tttacaccgc atccggcatt acggattctc
     6301 tctggttcaa cagagaatag tttgattaac gcaacaatgg acaatctggt ggtaatcgac
     6361 aacgcaacgc cgccggaagt atttcaatat ggaagttttg cccactcaac ggttcgccat
     6421 caagattaaa agtgatttcg tgtggggctt gtatatcaaa ccacgacgaa gctccttcga
     6481 taatattcgg gttatcttcg tcagatttta aggttgatac gagagtcgga ataatttcat
     6541 cgccggtaaa aatgcgcagt tgcagcaagc catcgttaat taacgcgttc gggcacaatt
     6601 gttgaccgcc accggcctga cgcccgttac caataccaat gaccagggcg tcaccttgcc
     6661 agtgaaagtt ttcaccgcgg atttcacaac ggtccggttg cagagtgtcc atgcgcatta
     6721 agccatgaat gatgtaagag acgccaccca gcgcggcttt taatttttcc ggcgtttctg
     6781 tggtaatacg cgtcccaaac ccgcctgtcg ccatattaat aaaacaggtt tgtttattga
     6841 cctgcgccat atctatcgca atggcgttac cggcaattgc cagtttcagc gccttatcca
     6901 gtgcctcagg aatccctaca ctggtggcaa aatcattggc ggttcctaat ggtaaaattc
     6961 ccagcgcggg tatgtcatcc ccctcacact gaatcaacgc cgtagaaact tcattaatgg
     7021 tgccatcgcc accaccggca atcaccgttg cgacgcccaa cttccgtgcc tcctctacat
     7081 atcgtgcggc atcgcctttc tcccaggtga cccgcacatg gatcgtcatt ccttcctcac
     7141 gcaacagcat aattgcttcg cgcaagggta gattgtcagt acttttgcca ttaagaatca
     7201 gtaagctggc gggaaattct gccatgatcg tatgtgcctt tatgattgga ctgtagatag
     7261 tgtagagcag aaaagaaaag gtggagtcag aacaggatga aagtcggagg ataaaaagtc
     7321 agttcaagta tggaggattc agtggttacc accaggagct tgcaatggga acagcgaata
     7381 ttaacggtag ccaccggtaa atttcaaaaa agaacatata ccta
//
LOCUS       DAZKGF010000204         7416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZKGF010000204 DAZKGF010000000
VERSION     DAZKGF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.204"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            109..477
                     /locus_tag="ACHOQV_004443"
     CDS             109..477
                     /locus_tag="ACHOQV_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632717.1"
                     /translation="MSAELKLVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
     gene            511..2169
                     /locus_tag="ACHOQV_004444"
     CDS             511..2169
                     /locus_tag="ACHOQV_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP9632718.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLSQ"
     gene            2171..3139
                     /locus_tag="ACHOQV_004445"
     CDS             2171..3139
                     /locus_tag="ACHOQV_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP9632719.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRVQRRFSALTALQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            3139..4002
                     /locus_tag="ACHOQV_004446"
     CDS             3139..4002
                     /locus_tag="ACHOQV_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9632720.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGMD
                     NPAQAIRNARENIKKSSSVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            4081..4506
                     /locus_tag="ACHOQV_004447"
     CDS             4081..4506
                     /locus_tag="ACHOQV_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9632721.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIELEMDRYVERERSGAQVSQFSGHTGN"
     gene            4706..4862
                     /locus_tag="ACHOQV_004448"
                     /pseudo
     CDS             4706..4862
                     /locus_tag="ACHOQV_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            5444..7153
                     /locus_tag="ACHOQV_004449"
     CDS             5444..7153
                     /locus_tag="ACHOQV_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP9632722.1"
                     /translation="MNGAVHYANALLQFTNYPDIIAIGMTGWRDDDTGELHHEIGVWY
                     VSKNNLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRL
                     SRLNNDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDI
                     IFRKIRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGE
                     YYKIGLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDF
                     ATGSAGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNM
                     ILMGDGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALS
                     MMDKGYAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQ
                     VKIPHNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLN
                     IFTEDEYFEGTIDPDSGEDWNQTRPVDTRPTLEDFKKTVGDYLAWEVSKLLQGRDASE
                     KKQ"
     gene            7186..>7416
                     /locus_tag="ACHOQV_004450"
     CDS             7186..>7416
                     /locus_tag="ACHOQV_004450"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9632723.1"
                     /translation="MKSGGKWEEYTIERLFSIQTPKKKFNANKLTFNGNYPYVARGSN
                     NNGIKGYIDEDELYLNPGNTISFGQDTATMFYQ"
BASE COUNT         2084 a         1568 c         1969 g         1795 t
ORIGIN      
        1 acgggatctt ccaacagacg ggtacagagg ttaggtagat ctcgatctaa aggggggcac
       61 ccctgaaaaa acggtcgtat cagcaaacta gtacatcatg gttaaaatat gagcgctgaa
      121 ctgaaactgg tactaatcaa ccttctggaa gaggaattcg gacatggaat ggacaaggtc
      181 acattcgact acgttctgca aaagaaaatc aaatccctgg gctgtgagtt gcaacgctgt
      241 ttcactgtca ggctgaaagg tgatcgcagg ggatttatcg atcttctggt tatttctccc
      301 gatggtcagc gttgcgcagt tgaggtggat aaccgatcgc cccgtcagcg ttcactgatg
      361 aaaattcgag cgcttcctga aggcatttcg ggatttgttt ttctccgtga tggcaaacat
      421 ccacagcgtt atatcgccga tggtgtggat gtcatccggg cgacccggtt taagtaatca
      481 ctgtatcagc cctgtcatcg ggagaatacc atgctgaata tcaagccgaa ttttgctcag
      541 gaacgtgcgc tgaatatgtt gcgtcatgac tggaaaacat atcagtcctt catgatgtac
      601 atgcctacag ggagtggtaa aaccggcctg gcggcgttca tcactgctgg tctggtaagc
      661 cgtggcatgc gcgttctgtt tgtcgttcct tataccattt tgatcaacca gacagcacag
      721 cgctttgttc agtacgggct ttccggggat gaaatcagct ttatctggcg tgatcatccc
      781 aataacgacc cgtcacgact gattcagatt gccagtgcgg atacgctgat tcgccgtgat
      841 tttcctcaga atatcgacct gctgattatt gatgaagcgc atctccgtaa gcgccgcatc
      901 ctcacggaaa tcgaacgact ggttgcagag ggaaatgtga aggtgattgg gttgtccggt
      961 acaccctttt catcgttcct ggggcgttat taccagcgtc ttctcaagcc gaccactata
     1021 ggggaactga tccagcgtgg cgaactgagc aattacgaat tttatgcgcc cacgaaaccg
     1081 gatctggaag gtgtaaaaac gaaagtctca atggagtacg gcagggatta cgacgaatca
     1141 caactggcag agattatgtg cggcgcggat ctggttggcg atattgtcga taactggctg
     1201 tgtaacggaa gggatttacc gacagtggcg ttctgcgtca ataaaacgca tgccagcttc
     1261 gtgaccatgc agttcaacaa agccggaatt aacgcagagg tgatggttgc tgaaacgcca
     1321 catgatgagc gtcagttaat gatcagtcgt tttgaaacgg gagcgacgaa aatcattgtc
     1381 agcgttggcg ttctggtggc gggatttgac agtgatgtcc ggtgcatcat ttacgcacgc
     1441 ccgacaaaat cagagattcg ctggcttcag gcgattggga gaggattgcg tacagcgcca
     1501 ggcaaggagt catgcctgat tttcgaccat tccggtacgg ttcatcgcct gggatttcct
     1561 gaaaatattg agtatgacga actgcccggc aaaaacgacg ggatgcaggt gtcatcatca
     1621 cgtggtaacg aaattcggga agaaaaactg ccgaaagaat gtccggggtg tcatttcatg
     1681 aaaccggcag gtgtttatgt ctgtccgaaa tgcggattta aacctctggc tggtgaggat
     1741 gtgaacacag atacccgccg caatatcagg aaactcagca aggacgaaaa aatttacacc
     1801 aaaagcgaca aacaggcctg gtggagtcag atcaaattct atcagcgtca gcgtgcctct
     1861 ctggggcgac cggtcagcga tggatggtgc gctcacactt tccgggagaa gttcggcgaa
     1921 tggcctgacg ggctgagcag ttttccgatg gaaatcaccc cggaggtcag taattacatc
     1981 agacacaaat tcatccggtt tgccagaggg cgtagccggg cagagaagat gacggaaaaa
     2041 tcccccgaag cgctttctct gtccctgctt catggtgtcc ggtctggggt gccggaaggc
     2101 agtgagtcgt ggcagatcat gcaggcaaag caacaacttc agaaaaacag aaacagtctg
     2161 agtcagtaag atgaaaacag cagaagcagc gaaaggccac tggcctgaaa ttttaaaaca
     2221 ttacggactg ccgccagtca cgggtaaaaa gcacttcaaa ggggaatgtc cggtttgtgg
     2281 tgccagaggt aagttccgta ttgatgaccg tgatggcacc ggaacgtgga tctgtgtttg
     2341 tggtagcggt gacggcatga aactgatcgc tctgacacaa aaaagagcgt ttcatgaaat
     2401 ttgcgctgaa atagaccgca tcacggggaa tgaataccgg agagataaac cgcctgtgat
     2461 ctgtacgtcg gaaagcctga gatcgcgcgt tcagcgtcga ttttcagcgc tcacggcgct
     2521 acagggaaca tctggtgctg aatatcttcg ttcccgtggc atattcagtc ttccggttga
     2581 gggagttcgc ttcaacagcc agcaaaatta taacgggcgc atgttccagt ctctttatgc
     2641 cctggcgacc gatgacaagg gagaactgtg ttacctgcat cagacattgc ttgatggagc
     2701 taaaaaagca gacattggta tcagcgccag aaggctcaaa tctttacagg acgataacta
     2761 tctggaccat gcccggtcag ttgctatcag gatgtttccg gtagccagta cactgggtat
     2821 agctgaaggt atcgaaaccg cgttgtccgc acatcagatt tacaaagtaa atacctgggc
     2881 aaccattaac agtggtttta tgaaaaagtt ccgcgtaccg gcgggagtca cccatctgat
     2941 tatttttgcc gatcgcgatg aatacagtgc taccggaatg gctgcggcct gtgaatgcgc
     3001 acatgcaaat ctccaggccc gaaatgacat tcagcgggta agtgtccggt ggcctgatca
     3061 tgatgatttc aacaatatgc tcatgaacgg cgatcaggtt cgggaactgg ttttttacag
     3121 aaaaaaggcg gttgcgtgat gcgtacggac aatcaggtac ataaagcttt attcactatc
     3181 ccgacggcag cgtacagcgc cgttccggca aatatcaaac ctctgccaga acaaaggagg
     3241 atcaccggac ataaacagac tgatgcttat ctctggattc tggaggttat tcacctgaac
     3301 gaggccgtac atctggacgc agccgaagca gcactggaga aactcaaaat aacgccggaa
     3361 gaagcgagtg aacggtatgg acgttatttg caggagatta atgttgatcc tttccagatt
     3421 gctttcgcaa ccataggcat ggataacccg gcgcaggcga tcaggaatgc ccgtgagaat
     3481 atcaaaaaat cctcatcagt cagggccaca tttggcagct atgaggcagc actcgacgat
     3541 gtggaggctg agcggataat tcgcacctcc ccgaaattta tcgacgatta ctactggggc
     3601 tggactgcgg cagagaaaaa agccggaagt attgacggtg ttcgctcaaa tgaaattgat
     3661 gatcagcgtc gtgcatatgt tgatggctat cgtgatgtac tgccagagcc tcatacattg
     3721 tcagacgttg ttcgtgagtt tatttactgg gactggcttt atgagatgcg ccagacagcg
     3781 gggagagaaa caggggacaa atacggtttc acaggtgaac atcatgagtc agtatatgat
     3841 cggcagttct ggctcgaaaa tttgctggga aaaataaagc ctgtgacgcg tgatgaagcg
     3901 gttgaagtgt gtcgctggtt tctggcaagc ggaaaagatg agtacatgga agacaatggt
     3961 tcagcggtca ttctcaatct ggttggggag tgtgagcaat gaagccggag atatccagta
     4021 gacattttcc ccctgagcat tcaaatcaga aaaagtatgg ttacagcgga gtacagaata
     4081 atgcgtgata tccaacaggt tctgtcccga tggggtgcat gggtggtaaa taatcatgag
     4141 agtgtggcat ggtccggcgt cgctgccgga tttaagggag tgatcccatc aaaggttaaa
     4201 tcacggcctc agtgtaccga tgatgatgcg ctgattatca gcaattgcat ggcgcaactg
     4261 aacgtcaaca acagcgattt gcatgatttt ctgtatgatt attatgtgtt cgggatgacg
     4321 cttatgggac tgggccgtaa gcatgagcgc tctgattgct gggccgggcg ggtactgcaa
     4381 aaagctgaag gtgttattga ggggatgttg attatgcagg gaatagaact ggaaatggac
     4441 agatacgttg agcgtgaacg atcaggggca caggtcagtc agttttccgg acatacggga
     4501 aattgaaagc gcggggtttt actgtagaat gactgcgggt gcttgagggt gtctgcctcg
     4561 ggcatgctgc cgtaaggcag acggagaaaa gccccagtta acattacgtg tcctgcaaga
     4621 cgcttaacat taatctgagg cccaatctat gtcacacaac cgtaggttag cctcttacgt
     4681 gccggaaggc aaggagaagc agtctatgaa gcagcaaaag gcgatgttaa ttcgccctga
     4741 tcgtcatctg tttaactgtc atagtgacgg cactggtaac gaggaaagac ctctgcgagg
     4801 tacggatccg aaccggccag acggaggtcg ctgtcttcgt agactacgaa tctgagaagt
     4861 aagagaccag gcgggggagt aatctcccgc cacctctgat gtgtcaggca tcctcaacgc
     4921 acccgtactt tatttgtcat atgctccgca aaatgcaggt tgtcgttgca acccgtgcgg
     4981 taattttttg tgtgcgagtg ctcaaaaaac gttgattttc ataaaaggat gattttatgc
     5041 tgccagacca gcgaccagcg accagcgacc agcgaccagc gaccagcgac cagcgaccag
     5101 cgaccgtaaa tcaagaaagt ctgtaaaatc tgttgaacca gacattgccg accgggctaa
     5161 cagctggctc aaatcttatg gacttgatta caaactggag caggcaccac ttaacaacga
     5221 gatcgataac gcactggatg cttatttttc gaaaaatggc ggcgcaggcg gtaatcgtcc
     5281 cgacgtaaaa ctccttgttc aggacaaata cggcaagcag tatcccgttc tcatcgagta
     5341 taaaggctac aaggacaggc ttatcagact tggtagcaac ggtattattg agaacaaaga
     5401 tgccagaaaa gaatggaatt ttaaaaatat taatggatac gcggtgaatg gcgcagtcca
     5461 ttatgccaac gcacttcttc agttcaccaa ttaccctgat attattgcta ttggtatgac
     5521 cggctggcgc gatgatgata caggcgaact gcatcatgaa atcggcgtgt ggtacgtatc
     5581 aaagaataat ttaggagccg ggcagaaggt tggtgaattc accgacttat cattccttgc
     5641 tgacaaaaat gttgatggat ttctcaacaa gataaagctg cttaatcttc ctcccgaaga
     5701 actggaaaaa atcaaggcca gcaaagagga agagatagac acgcgacttt cccgtctgaa
     5761 taacgatatt taccagaatg aaaaggggct tggtgaaagc gatcgtgttt accttgttgt
     5821 ggctaccgtt attgcaacgt tggggattcc gggaaagtta gcaccgctgg ataaaaaaga
     5881 actgacctct tcaacggagg aggacttgcg tgatggtgat attattttca ggaaaataag
     5941 gaatttttta aggctgaaag ctgttcctga aacaaaaaga gaaatgattt tgcgctcgtt
     6001 gcaaaatacg ctgtggactg aaaacattaa caaaccagtc aacggtgaaa gccagttaaa
     6061 gcgcgtgttt gttaaagttg ttgatgatct tggggagtat tacaaaatcg gactgaccac
     6121 cgattttacg ggcaaactgt ttaatgagat gtatcgctgg cttggattta cgcaggacaa
     6181 actcaatgac gtcgttctga caccacctta tgttgccaca ttgctggcac gactcgccag
     6241 agtaaacaaa gattcctatg tgtgggattt tgctacaggt tctgcgggat tgcttgtggc
     6301 tgcaatgaat gaaatgctca ttgacgccag agaaaatatt cactcaccga atgaactaca
     6361 acttaaggaa gcgcaaatca aggctgaaca gcttcttggc cttgaggtgt tatccagtat
     6421 ttatatgctg gccatcctga atatgatttt gatgggggat ggtagctcga atatcctcaa
     6481 taaagactcg ctggccgatt ttgacggcaa gtatggattt gggaaaacag gagaaaaatt
     6541 ccctgcggac gcatttattc tcaatcctcc gtattcagct aaaggtaacg gcatgatttt
     6601 cgtgcagaag gcgctgtcga tgatggataa aggctatgct gcagtgatta tccagagttc
     6661 agccggcaca ggaaaggcta cggagtacaa caaaaaaata ctcaaggaaa ataccttgct
     6721 ggcaagcatc aaaatgcctg cggatctgtt tattggtaaa tcaagcgttc agacgtacat
     6781 ttacgttttt caggtaaaaa ttccgcataa cgcgaaacag gctgttaagt ttatcgactt
     6841 ttccaatgac ggctatgctc gctctaatcg caaaaaggca agaaacaatc ttgttgatgc
     6901 cgatcgtgca aaagagcgtt atcaggaggt tgtggatctg gttcacttcg ggaaaggttg
     6961 ccttaatatc tttacggagg atgaatattt tgagggaaca atcgatcctg atagtggtga
     7021 ggactggaat cagaccaggc cagtagatac cagaccgaca ctggaggatt ttaaaaagac
     7081 ggttggcgac tatcttgcgt gggaggtgtc aaaacttctt caggggcgtg atgcgtcaga
     7141 aaaaaagcag taaactcccg tcttgcagag cttgagcggg agtttattaa atctggtggt
     7201 aaatgggaag aatacacaat cgaaagattg ttcagtattc agacacctaa aaagaaattt
     7261 aatgccaata aattaacatt taatggcaac tatccttatg ttgccagagg aagtaacaat
     7321 aacggcataa aaggatatat agatgaagat gaattatatc tcaacccggg gaatacaatt
     7381 agctttggtc aggatactgc gaccatgttc tatcag
//
LOCUS       DAZKGF010000205         7413 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZKGF010000205 DAZKGF010000000
VERSION     DAZKGF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.205"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..236
                     /locus_tag="ACHOQV_004451"
                     /pseudo
     CDS             1..236
                     /locus_tag="ACHOQV_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(325..1461)
                     /gene="yibH"
                     /locus_tag="ACHOQV_004452"
     CDS             complement(325..1461)
                     /gene="yibH"
                     /locus_tag="ACHOQV_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9632724.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1464..1826)
                     /gene="yibI"
                     /locus_tag="ACHOQV_004453"
     CDS             complement(1464..1826)
                     /gene="yibI"
                     /locus_tag="ACHOQV_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP9632725.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2363..4276
                     /gene="mtlA"
                     /locus_tag="ACHOQV_004454"
     CDS             2363..4276
                     /gene="mtlA"
                     /locus_tag="ACHOQV_004454"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFP9632726.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4641..5789
                     /gene="mtlD"
                     /locus_tag="ACHOQV_004455"
     CDS             4641..5789
                     /gene="mtlD"
                     /locus_tag="ACHOQV_004455"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9632727.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIRK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5789..6376
                     /gene="mtlR"
                     /locus_tag="ACHOQV_004456"
     CDS             5789..6376
                     /gene="mtlR"
                     /locus_tag="ACHOQV_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFP9632728.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6387..6596)
                     /gene="yibT"
                     /locus_tag="ACHOQV_004457"
     CDS             complement(6387..6596)
                     /gene="yibT"
                     /locus_tag="ACHOQV_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFP9632729.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            6880..7242
                     /gene="yibL"
                     /locus_tag="ACHOQV_004458"
     CDS             6880..7242
                     /gene="yibL"
                     /locus_tag="ACHOQV_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFP9632730.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1890 a         1859 c         1853 g         1811 t
ORIGIN      
        1 gtgatctata acttaaatat ttttaaatgt gactgtcatc cttcagataa taaagccatc
       61 atcgcaatag atggaaaagc actcctgctc ttttttgatt agagccgtgg cagaggaaca
      121 actcatatca tcattgcgat ctcgacaatg tatagtctga tccttcggca gatcaagacg
      181 gataaaaaat ccaatgagtt cacactcatc ccgaacttct taacataatg gatattaacg
      241 aaaaatcatc acagccgatg aaataatatg caaaaataat tagtccttac aaggagcaaa
      301 gttccataat ctcccccctc cccctcaatg atccaaataa agataatgca tccaactggt
      361 cattctcagc agcactttcc gcatcaccga aacatggctg aaatggtcgg agtaaactgc
      421 cacttgggcg tagatgccgt cgggtaaggc atcgatatca tcgttagggt ccagttcaat
      481 ggttcccagc acaccgtccg tgccaggcac gaccgttaat gattgcaata ccccctgcgc
      541 ctgataagaa ccgcctggca cgacaggtaa aatactagtc agtttgccgt gaaacacctg
      601 cccaggtagc gcgttaaaca ccacttccgc atcatcgccg ggtttcagac gtaacagcga
      661 gttttgccga aattgggcga caatttgccg tttttgctcg gggatgaaaa ccatcaccgg
      721 acgcaacggc aaggcagctg cgtatgtacc tgggcggatc agtacctgag taacgtagcc
      781 attgcttggt gcgcgaatga cagtctgctc aaggttatat tttgcttcag taagttgcgc
      841 tcttaagctc acaatctgcg attgctcgcc gttaaccata ctgtcgagct ggctctggat
      901 ctgcgcctgc tccgccaccg agcctttcac cagcgcgtcc tgcgcgagga aattttgccg
      961 cgcatcgtcg atgtcacgtt ccgagaacgg attcaccgcc gcctggctgc ctttcaggta
     1021 acgttgataa tttttaaaca gacggtcgcg ctccgctgaa acctgggtgg tgttggcctg
     1081 cgcttcagtg agctgcgcgc gcagcgtctt tatattatgc gtcgccgtca tcaggtcagc
     1141 ctgaagtctg tcaactcgcg cctggtaacg aaccgggtcg agcttaaaaa gcacctcgcc
     1201 cttttgaata agctgattat ttttgtcggt gacttcagta acaattcccg tcacctgtgg
     1261 cgtgatgggg atcgctatca ctgccttttg cgcggtaaaa gtgtaagggt ggttgtagtt
     1321 catcaacaaa atcaaaccac tcaccagaaa cacgcctccc agcgccgcgg tcgccagcgt
     1381 ccactgattc accggaatgc ggaagatttt aaagaccgcc cacgccagcg ccacgtaagt
     1441 taaaacaatc aatagatcca tatttagatc tccgggaacg tggaatagtc agtctttttc
     1501 tcggcggcga gttgatgctc cagccgggaa attcggtcag aaagtgcggc gatttccggg
     1561 tcagtcgctt tctcctgcga cgcaacatgt gactgcatac cccagccacg ctccggttga
     1621 tagagcgtcg cccagatcca caggaacggc cagataacat gcagagtaaa caggctcacc
     1681 cagcccgccg tatgaatagc gtcggcatgg ggatgattgc gctttttggc aatcagatat
     1741 gggatgtcat gtatcgcgat gattccataa aaaatcacca gaaagacgaa gatcagcact
     1801 cccaacgcga aatagtttag aaacatatct gcctcggatt cacgtttatc agtgttgttt
     1861 tttggctggc agccagaagg gagtcaggct gatattttga caataatccg ggttcgcgat
     1921 tctcgccata acaccaaaga ataattttta gaggtgatga gttgcttagt tacataacga
     1981 ttttatgacg aaggcataac atgctgtaga tcacatcagg tgaacgccgt aagaaaatat
     2041 cttgtgattc agatcacaaa gatccaacaa accaccaaaa caaaaatgtg acattagtca
     2101 catttaaatg ccatttttag cgaaaatcgc cgtcttgttg cttttttaca caagcatttt
     2161 gtgatgagca tcacgtcaat tacctctcta ccccctatat ttatgtgacc catatcacac
     2221 aaaaggccgt cgactggaca gttaaccgat tcagtgccag atttcgcagt atctacaagg
     2281 tccggctacc tctgccgcca cattaacaaa aaacctcggg cttccagcct gcgcgacagc
     2341 aaacataaga aggggtgttt ttatgtcatc cgatattaag atcaaagtgc aaagctttgg
     2401 tcgtttcctc agcaacatgg tgatgccaaa tatcggcgcg tttatcgcgt ggggtatcat
     2461 caccgcgtta tttattccaa cagggtggtt accgaacgag acgctggcga agctggtcgg
     2521 gccgatgatc acttatctcc tgccgctgct gatcggttat accggtggta agctggtagg
     2581 cggcgaacgt ggcggcgtag tcggtgccat caccaccatg ggcgttatcg tcggcgcaga
     2641 catgccgatg ttcctcggtt ctatgattgc aggtccgctg ggcggctggt gcattaagca
     2701 cttcgaccgc tgggtagacg gtaagatcaa atccggtttt gagatgctgg tgaataactt
     2761 ctccgcaggc atcatcggga tgatcctcgc tattctggca ttcctcggca ttggcccgat
     2821 tgttgaagcc ctgtccaaaa tgctggctgc gggcgttaac ttcatggttg tccatgacat
     2881 gctgccgctg gcgtctatct ttgttgaacc ggcgaaaatc ctgttcctca acaacgccat
     2941 taaccacggt atcttctcgc cgctgggcat tcagcagtcc catgaattgg gtaaatccat
     3001 cttcttcctg attgaagcta acccaggtcc aggtatgggc gtgctgctgg cgtacatgtt
     3061 ctttggtcgt ggcagtgcta aacaatctgc gggcggtgcg gcaatcatcc acttcctggg
     3121 tggtatccac gaaatttact tcccgtatgt gttgatgaat ccgcgtctga tcctggccgt
     3181 tatcctcggc ggtatgactg gcgtgttcac gctgactatc ctgggcggtg gtctggtttc
     3241 tccggcatct ccgggttcta tccttgctgt actggcgatg acaccaaaag gtgcttattt
     3301 cgctaacatc gcaggtgtgt gtgcggcgat ggctgtctcc ttcgttgtct ctgccattct
     3361 gctgaaaacc agcaaagtga aagaagaaga tgatattgaa gcagcaactc gtcgtatgca
     3421 ggacatgaaa gctgagtcta aaggtgcgtc tccgctgtct gcgggcgatg tgactaacga
     3481 cctgagccac gtacgtaaaa tcatcgttgc ctgtgacgcc ggtatgggtt ccagtgcgat
     3541 gggcgcaggt gtgctgcgta agaaaattca ggatgcaggt ctgtcgcaga tttcggtcac
     3601 taacagcgcg atcaacaacc tgccgccaga tgtggacctg gtcatcactc accgtgacct
     3661 gaccgaacgc gctatgcgcc aggttccgca ggcacagcat atttcgctga ccaacttcct
     3721 cgacagcggc ttgtacacca gcctgaccga acgtctggtt gctgcccagc gccatactga
     3781 aaacgaagta aaagtgaaag acagtctgaa agacagcttt gacgattcca gtgctaacct
     3841 gttcaagcta ggcgcggaga acatcttcct cggtcgcaaa gcggccacca aagaagaagc
     3901 gattcgtttt gctggcgagc agctggtgaa aggcggttac gttgagccag aatacgttca
     3961 ggcgatgctg gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc
     4021 acacggtacg gttgaagcaa aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta
     4081 cccggaaggc gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat
     4141 tgctgcccgt aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga
     4201 tgaatccgtc atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgct
     4261 ggcaggtcgt aagtaataca atctcaccct ctccacatgg agaaggtggg gcttattgcc
     4321 tgatgcgcta cgcttatcag gcctacaaaa caaagcacag tttgttgaat ttgcacgttc
     4381 ttgtaggccg gataaggcgt ttacgccgca tccggcgctg cccctctcca catggagaag
     4441 atgggctaat tgcctgatgc gctacgctta tcaggcctac aaaaacaaag cacaatttgt
     4501 tgaatttgca cgttcttgta ggccggataa ggcgtttacg ccgcatccgg cgcggcccct
     4561 ctccacatgg agagggtttg ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa
     4621 cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat atcggtcgtg
     4681 gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc
     4741 aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg gtcggtgaaa
     4801 ccgagcaggt agataccgtt tccggcgtca atgccgtcag cagcattggt gatgatgtcg
     4861 ttgatctgat tgctcaggtt gatttagtca ctaccgccgt tggcccggtt gtgctggaac
     4921 gtattgctcc ggcaatcgcc aaagggctgg tgaaacgtaa agaacaaggt aatgaatccc
     4981 cgctgaacat catcgcttgt gaaaacatgg tccgcggcac cacacagctg aaaggccatg
     5041 tgatgaacgc cctgccagaa gatgccaaag cgtgggtaga agaacacgtt ggctttgtcg
     5101 attccgccgt tgaccgcatc gtgccgcctt cggcttctgc aaccaacgat ccgctggaag
     5161 tgacggtaga aacattcagc gaatggattg tcgataaaac gcagttcaaa ggcgcgctgc
     5221 caaacatccc tggcatggaa ttaaccgaca atctgatggc atttgtcgaa cgtaaactct
     5281 tcaccctgaa tacaggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga
     5341 ccattcgtga tgcgattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag
     5401 aaagtggtgc agtattgatc aagcgctacg gctttgacgc agacaaacat gcggcgtaca
     5461 tccggaaaat cctcggtcgt ttcgagaacc cgtacctgaa agatgatgta gagcgcgtag
     5521 gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct aatcaagcca ctgctcggaa
     5581 cgctggaata tggtctgcca cataaaaacc tgattgaagg tattgccgct gcaatgcact
     5641 tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc
     5701 cgcaggcggc gctggcacag atttccggtc ttgatgccaa cagcgaggtt gtatccgagg
     5761 cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc
     5821 gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg
     5881 tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga
     5941 gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc
     6001 ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg atctttctgt
     6061 gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga
     6121 actgctgatg gcattgcgtg aagagctaaa tcacgacggc aacgagtacg cgtttaccga
     6181 cgacgaaatc cttggaccct ttggtgaact gcattgcgtg gcggcgttac caccgccgcc
     6241 acagtttgaa cctgcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc
     6301 tgtgcgatca acaatggtcc tttcactgac agagctgatt tccaaaatca gcttaaaaaa
     6361 agcctttcaa aagtaagtac tttcgcttac ttctcctcta cctgtttcgg acgataaagc
     6421 tgacgataat actccagacg ttgtagatat aacggcacgc tttcatcagg tattccggac
     6481 ggaatcgcgt tacggggagg tagttttttc agatactccc ggaacgcctg gctcgatgcc
     6541 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     6601 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt tagatcccgg
     6661 tataaatgac attaaggaaa acgtgctcaa tcctcaataa tcacctgctt ctgttgacta
     6721 tcttttttct ttaaatttct taattaatca ataaattgcg tagtttttaa gcatgttaat
     6781 gtttacgtta tagaactatt gcaatgtcat cagttgtgaa caaagcacct ggtcgcgcat
     6841 actaggggct ataaatttat ctttatcaga agccatcaca tgaaagaagt cgaaaaaaac
     6901 gaaatcaaac gtctcagcga tcgtctggac gccatccgcc accagcaggc cgatctgtcg
     6961 ctggttgaag ccgcagacaa atatgccgaa ctggaaaaag agaaagccac tctggaagca
     7021 gaaattgctc gcctgcgcga agttcacagc cagaaactca gtaaggaagc acaaaagctg
     7081 atgaagatgc cattccagcg cgcgattacc aaaaaagagc aggctgatat gggcaagctg
     7141 aagaaaagtg ttcgcggact ggtcgttgtg cacccaatga ccgcgctggg ccgcgaaatg
     7201 ggcctgcaag agatgacagg gttctcaaag actgcgtttt aagttccttt ctcgtcgctg
     7261 taatcacagc ctctcaccat ttactcgctc ctgctggcac atgcaggagc ctgtttcaga
     7321 gatcgaatat ttataacgtt cttaacatta tatattcaat ctcctctatt ctttttagcg
     7381 cacatgccag gctaaattct gaaaatcagg tac
//
LOCUS       DAZKGF010000206         7308 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZKGF010000206 DAZKGF010000000
VERSION     DAZKGF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.206"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..1700
                     /gene="mppA"
                     /locus_tag="ACHOQV_004459"
     CDS             87..1700
                     /gene="mppA"
                     /locus_tag="ACHOQV_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFP9632731.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKAAPDQLGVTA
                     VDAQTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPVYDKVLAQASTENTVKARNADYNTAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1751..2782)
                     /gene="ynaI"
                     /locus_tag="ACHOQV_004460"
     CDS             complement(1751..2782)
                     /gene="ynaI"
                     /locus_tag="ACHOQV_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFP9632732.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            3026..3283
                     /gene="ynaJ"
                     /locus_tag="ACHOQV_004461"
     CDS             3026..3283
                     /gene="ynaJ"
                     /locus_tag="ACHOQV_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFP9632733.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(3333..4283)
                     /gene="uspE"
                     /locus_tag="ACHOQV_004462"
     CDS             complement(3333..4283)
                     /gene="uspE"
                     /locus_tag="ACHOQV_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFP9632734.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(4435..5187)
                     /gene="fnr"
                     /locus_tag="ACHOQV_004463"
     CDS             complement(4435..5187)
                     /gene="fnr"
                     /locus_tag="ACHOQV_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFP9632735.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(5382..5897)
                     /gene="ogt"
                     /locus_tag="ACHOQV_004464"
     CDS             complement(5382..5897)
                     /gene="ogt"
                     /locus_tag="ACHOQV_004464"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP9632736.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISAANPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(5908..6051)
                     /locus_tag="ACHOQV_004465"
                     /pseudo
     CDS             complement(5908..6051)
                     /locus_tag="ACHOQV_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            6051..6267
                     /locus_tag="ACHOQV_004466"
                     /pseudo
     CDS             6051..6267
                     /locus_tag="ACHOQV_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(6441..7256)
                     /locus_tag="ACHOQV_004467"
     CDS             complement(6441..7256)
                     /locus_tag="ACHOQV_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9632737.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
BASE COUNT         1994 a         1690 c         1714 g         1910 t
ORIGIN      
        1 cacttttttg atggaagttg tttgcatttc tttaaggcga aacaaataat tacgcatcaa
       61 ttttaatgtc ggttagaggg aaacttatga agcactctgt ttcagtcacg tgttgtgcgc
      121 tgttggtcag cagcatttct ctttcgtatg ctgctgaagt tccgagcggc acagtactgg
      181 cagagaagca ggagctggtg cgccacatta aagatgagcc tgcgtcgctg gatcccgcta
      241 aagccgtggg cctgccagag attcaggtca ttcgcgatct gtttgaagga ctggtgaatc
      301 agaacgagaa aggggagatt gtccccggcg ttgcgactca gtggaaaagt aatgacaacc
      361 gtatctggac ttttaccctg cgcgataacg caaaatgggc ggatggcaca ccggtaacgg
      421 cgcaagattt tgtctacagc tggcaacgtc tggtggaccc aaaaacattg tcgccatttg
      481 catggtttgc cgcgctggcg ggaatcaaca acgcacaggc gattattgat ggtaaagccg
      541 cgcctgacca gcttggcgtc accgcagttg atgcccaaac tttgaaaatc cagcttgata
      601 aaccgttgcc gtggtttgtg aatttaaccg ctaactttgc cttcttcccg gtgcaaaaag
      661 ccaacgtaga aagcagtaaa gagtggacga aacccggaaa tctgatcggt aatggcgctt
      721 atgttcttaa agagcgcgta gtcaatgaaa aactggtcgt ggtaccgaat acccattatt
      781 gggataacgc caaaacggtg ctgcaaaaag tgaccttcct gccaattaat caggaatccg
      841 cagccactaa gcgttatctc gcgggggata ttgatatcac cgaatccttc ccgaaaaata
      901 tgtatcagaa gctgttgaag gatattccgg ggcaggttta tacgccgcca cagctcggga
      961 cctattatta tgcgtttaac acgcaaaaag ggccgacggc cgatcagcgt gttcgcctgg
     1021 cattaagtat gacgatagat cgccgcctga tgaccgaaaa agtgttggga acgggcgaaa
     1081 agccagcgtg gcattttaca ccagatgtta ctgcaggatt tacgccggaa ccttcgccgt
     1141 ttgaacaaat gagtcaggaa gaactgaatg cgcaggcaaa aactttgttg agcgcagctg
     1201 gttatggtcc gcaaaaaccg ctgaagctga cgcttttgta taacacttca gaaaaccatc
     1261 aaaaaattgc gattgctgta gcatcaatgt ggaaaaagaa ccttggcgta gatgttaaat
     1321 tgcaaaatca ggaatggaaa acctatatcg atagccgtaa caccggcaat tttgatgtta
     1381 tccgagcctc gtgggtaggg gattataatg aaccctccac tttcctgaca ttattaacgt
     1441 caacgcattc aggaaatatt tcacgcttta acaatccggt atacgacaaa gtactggccc
     1501 aggcatcaac ggaaaatacc gttaaagcgc gtaatgccga ttacaatacg gcagaaaaaa
     1561 tcctcatgga gcaagcaccg attgcaccaa tttatcaata taccaatgga cgattaatca
     1621 agccgtggct gaaaggttat cccattaata acccggaaga tgtggcgtac agtcggacta
     1681 tgtatattgt gaagcattga tgtgatggga actggcgtta cccttgtgga taacgccagt
     1741 gatgtctgat ttagcgaccc tggtccggcg gtgtaatgtt atccatatac agcgtctggc
     1801 tggggaaggc aaagtccgcg ccgtgtgact gtacaatatc gataatcttc aaataaacgt
     1861 cttgctgtgc agcaagccat tcagcccata ccgtggtttt ggtaaagcaa taaaccataa
     1921 tattcaatga agagtcagca aactggttga aataaaccag taaggtttgt cgctggtcga
     1981 tggccgggtg atttttcagc atctcacgta cggcttcgac aataacgccc acttttgccg
     2041 catcctcata acgtaaacca atggtcgtgg taatgcgccg gttggtcatt cgtcctgggt
     2101 tttctacgct gatcgacgaa aacagcgagt tcggtacgta caacggacga ttatcaaaag
     2161 tcgtaatttt ggtaattcgc cagccaattt ccgctactgt accttcgata tttctgtccg
     2221 gtgaacggat ccagtcgcca atactaaaag gacggtcgaa atagagcata atcccggaaa
     2281 agaagttact caaaatatct ttaccggcca taccgacagc cagaccacca ataccaccaa
     2341 aggtcaacaa gccagaaagg ctcataccga aatgttcacc ataaagcaga acaagcacca
     2401 caataatggt gattttgatg atacgcgaca taatccgcgc actggtgata tcgcgacctt
     2461 ttttaatctg ctgtttttca aactgattta tcagcagaaa tagcttaatc gtcagaataa
     2521 ccgcaatcag ggacgtacag ataaaatcga taacgcctgg ggtgataaat ttgagtttat
     2581 agttttctat aacataatta atgatgctac caacagcact gataattatg gtgtagatta
     2641 aaaattgcac cgcatggaat aaaaatcctt ttcttttacg atttccgcgg cgaaaccaaa
     2701 agctcatcag aatcaatgct gcgcagctac cgaaaataat gaccagatta agcgcattat
     2761 ttgtaaacag ttcagcgatc attgttttat caggctcctc cagataattg tcgtcatgcc
     2821 ggaaacccct ggcggggcta ttttaccgcg acaattcatt cagatcatca atagtcaggg
     2881 aaggaagtag caacattagc taactaatca atgcgttgat tgtaaatcca gctaagaggt
     2941 gaggttttca gagcagacaa cggtgaaatg tcatggtatt gttacgttta ggtaacaaga
     3001 aatttgtctg cacaaggatt acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa
     3061 gaaatttctc tttggtttgt tggcggtttt cattattgcg gcgtcggttg tgactcgcgc
     3121 gaccatcggc ggcgtgatag aacagtacaa tattccgctg tctgagtgga cgacatcaat
     3181 gtatgtgatt cagtcatcga tgatttttgt ttatagcctg gtctttactg tgttgctggc
     3241 aatcccgttg ggaatttatt tccttggcgg cgaagagcag taagtaaaaa ataggcccga
     3301 taactcgggc cttgtcagtt attgaagagt cgttaatcgt cttcttcgtc atccagttca
     3361 acgggtgtct gatactggtc aggtttaatg accagcaggt cgcagcgaag atgatcaatc
     3421 acctgttccg ccgtgttacc aaggaatgct gctgaaatac cggtgcgtcc gaccgtgccc
     3481 agaaccacaa tccccgcctg taagtgctcc gccaaatcag gaatcacctc ttctggcaga
     3541 cctttttcta cgtgcgtcat gttttcatta atgccgaatt tctgccgcag ggctttcatt
     3601 gccagcaaat gttgcccacg aatggcatcg ttataaacgc tcggatcaaa ttccggcagt
     3661 tcaatcgcga tattaattgg cgttaccgga taagcgccaa ccagatgaac ttcggtatgg
     3721 ttgacttgtt ccgccagttc gatcgtctct ttgaccagtt tttcattgag cgcattatga
     3781 tacggctctt cactggcgag attcaccgcc accagcgcct tacccccttc cggccacggc
     3841 tggtctttca ccatccacac ggggcttggg catttgcgta acagatgcca gtccgttggc
     3901 gtaaaaatca ccgcttccag acggtcatgt tggtgcgcca tttttagcac caaatcgtgc
     3961 ccgccgctga tcacttcctg aatgatggct tcgaaaggac ggttatgcca gaccacttta
     4021 atttcaatgg gaacgccagc attgagataa tattttgcct gctcgtggat ccaggctgta
     4081 cgctggctga tgacgccctg acgcatagcg gtacgttcgt ccggggagag cagggtggtc
     4141 atttcgtatg agaagtcata gatcggcaaa aaggctttaa ttttgccacc aatccgttga
     4201 tgtaaataaa cagctcgccg caatgctggt tggtcgtcct ggttaggatc gataacaacg
     4261 agcatgttct gatacatagc catacagggt ctccttacaa caactgtcaa cgcagtttgt
     4321 aattaaaaga ttaacccata tctggtgaat gaaacagtga tgaaccttct gccagatcaa
     4381 taaatcagaa aaatttaatg atatgacaga aggatagtga gttatgcgga aaaatcaggc
     4441 aacgttacgc gtatgaccag caagctgggc cagcgcatcg ttattttcga tggtgatgta
     4501 tttacctttg actgccagca taccgctttt ctggaagcga cccagcagac ggctgatggt
     4561 ttctaccgtc aggcccagat agttaccaat atcgccacga gtcatcgtca ggcggaattc
     4621 acgaggggag aagccgcgtt gggcaaaacg acgggacagg ttgtagatga atgcagccag
     4681 acgttcctcg gcatttttct tcgacaacag caggatcatg tcctgatcgc ctttgatttc
     4741 accgctcatc agacgcatca tctgctgacg cagattcggc attttaccgg acaaatcgtc
     4801 cagcgtttcg aacgggattt cacataccat cgaggtttcc agcgcctgcg cgaagctcgg
     4861 gtgatggccg ctgccgatgg cgtcaaatcc caccaagtcg cctgctaaat ggaaaccagt
     4921 gatttgctcg tcgccttgct cagtgatggt ataactttta atcgtaccgg agcggatggc
     4981 ataaagcgat ttaagttcat caccagcctt aaacagcgtc tggcctttct gaataggctt
     5041 cttccgctca atgatattat caagctgatc aagctcatgt tcgttgagtg tgaacgggat
     5101 gcaaagctgg ctgatgctgc aatcctggca atggatagca caaccgccag actgaatgcg
     5161 ccgtataatt cgcttttccg ggatcatagg tctgctcaag ccgtaattga tatttgtcaa
     5221 ttttaacatc tttttaggga gcaagtaagt ctaagcaaac cttaacagca gagaattccg
     5281 atattagatg taaatatatg tctatctatt tgaaaaccct taagttgtta agggtaactt
     5341 tacataaaag tgtgaacaag ctggcacaaa ttgtttaatg tttacagcaa aagataacct
     5401 tcgtggcgca ataaccactc ttttcgctga actccgcctg catatccggt catggtgccg
     5461 tttcggccaa taacccgatg gcaaggtaca acaatgctga tgggattcga tccgttagcc
     5521 gcaccaacgg cacgcgccgc gccagggcga cccaattgtt cggccagttg accgtaatgc
     5581 attacctgcc cgcaggggat agtgcgtagt gttttccaga cttcgcgctg aaatggcgtc
     5641 ccccccgtgg cagtgggaag cgtatcaata atgctaagat taccggcaaa atattcacga
     5701 agcttgtcgc ttaaaccacc tgggttggcg gcagaaatgc gctcatagcc ttctttgcga
     5761 taatggatgt ccagcagctg caccatgcgt tcgctgtact cttcccattc aaccgcccgc
     5821 aggcgaaatt gctcatcgca aatcacccat agtggaccca gtggcgtggc aattttttct
     5881 tcaagtaatc tcagcatccg ttctctctta agacaaacgt gggtaaatcc ccggacctat
     5941 cggcagacca acaagatacc acgccagcaa catcagcagc cataccacca aaaagataag
     6001 cggatagggc aagactaacg aatagtaagt acccagtttc gcgtctggtt tgtcgcagtc
     6061 tgatagcata aagcgatttt ttgatttttc tgcctgaaga aatgagggca agccatcaca
     6121 tggatcgagg gtgggataat tccagtcagc gaaattttac cgtaggctat ctgttatttg
     6181 attctgtggc gtgatagccg ccagacacca ctgatctcgt cactcattcc ccaatcccgg
     6241 cgagaatgtg gttatttaca gagctaaaaa atgaataaaa aaatagagcc tgtcgacatc
     6301 cgccagactc tacagtacac acagcagtgc atcctcgtct taaatccggc ctggggtcct
     6361 ggtgaccggt gacagtgtta taacggtttt acccatccgt tgccaatttt cttcagtgac
     6421 agatttcacg aaaatataag ttaaatcatt tgccttgtga ttgcataatc atgcatcgtt
     6481 aatcgacgag taaatttacc gggcgagtag cccgttcgac gcttaaacat attgccgaac
     6541 gattcagctg aggcatagcc cagacgatgg gcaattttgg tcaacgaata tccatcatca
     6601 agaagcgtca ctgcagaaat aacgtgcatt tgctgaaccc agtggcgaaa tgttaatccc
     6661 gtctctttaa caaataagcg catgagtgta cggctactga cgttaatttt atccgctaga
     6721 ttatccagtg ttggcagata cccttggttg tttagcaaat tctcacaaag ctgctgtagc
     6781 cgctcatcat ttggccaagg gagttcaagt gacattttca cccccatttt tatttcctga
     6841 agtatcagtg tctcgagggc tgaaaaacgt aactgttgtt tatctgtggt actttgcgac
     6901 ggtggaagtt ggttaattgc aattagtaat tctcttaaca ggggtgaaat acccgtcatg
     6961 aagcaaactt ttcccagcgt ttcactttcc gcttctgaaa ccagtacggt ttttaaaagt
     7021 acgttgcctg tcgcaacaaa cttatgcggc atttgtgggg gcagccaaag cgcgctgctg
     7081 gcatgcacta gccagacgct ttctccagcc catacacgaa cgactccttc agccgggtaa
     7141 agcaactggg catagagatg tttatgccag ggaactgtgc ctccattcag atgttccgaa
     7201 tccagtccga taatgtgatg ccctggtgat gctttgggtt gcagtgtgat tgtcatttac
     7261 agtaggtatt ttgtcagaaa tcatcaaata agccatgtta aattttaa
//
LOCUS       DAZKGF010000207         7186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZKGF010000207 DAZKGF010000000
VERSION     DAZKGF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.207"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(143..820)
                     /gene="tli1"
                     /locus_tag="ACHOQV_004468"
     CDS             complement(143..820)
                     /gene="tli1"
                     /locus_tag="ACHOQV_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFP9632738.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKKLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDTTRPSQVSVGRWNDHLGATESYYNK
                     GKALPLMLRFCWDSIIDKKSYETLIFFKKGTWEQMVTPYTDNRWNETYYRSTMIIGLA
                     PEGKVRVWLGDRGNPVVPQLDAKITTVSGDKMKMCKGVTKSDFSYGYDEDIKEFIRDK
                     TYPYGNW"
     gene            complement(840..2522)
                     /gene="tle1"
                     /locus_tag="ACHOQV_004469"
     CDS             complement(840..2522)
                     /gene="tle1"
                     /locus_tag="ACHOQV_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HFP9632739.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNSISNGSYRGYYSNI
                     HWLNSLYVADQTLLESQVEAQIQTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSVV
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PEFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFEPIRQTNTELQLPSE
                     LIFLADKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            complement(2519..5038)
                     /locus_tag="ACHOQV_004470"
     CDS             complement(2519..5038)
                     /locus_tag="ACHOQV_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP9632740.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEVVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKVTGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            complement(5117..5344)
                     /locus_tag="ACHOQV_004471"
     CDS             complement(5117..5344)
                     /locus_tag="ACHOQV_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632741.1"
                     /translation="MKIKRTLLIMLSRFIRGAGMGLGASGIVFTVWFFFLSSAEFKYL
                     WGGLSVALFFVGYLMYRFAYAYIYDEWDDYQ"
     gene            complement(5341..5982)
                     /locus_tag="ACHOQV_004472"
     CDS             complement(5341..5982)
                     /locus_tag="ACHOQV_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632742.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTRHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            complement(5969..>7186)
                     /locus_tag="ACHOQV_004473"
     CDS             complement(5969..>7186)
                     /locus_tag="ACHOQV_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9632743.1"
                     /translation="ELMTARDDLEQQYARECELTGELLESRSDISRQSETLHLQQALH
                     DIQQNQPLLSVDVDVRTVAGVVADRTGVPLSSLMKDEQTELLHLEKDIGRRVVGQDVA
                     LESIAQRLRAANTGLTSGNGPQGVFLLVGPSGVGKTETALALADVMYGGEKSLITINL
                     SEYQEPHTVSQLKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVEKAHRDVLNLFYQ
                     MFDRGFMRDGEGREIDFRNTVILMTSNLGSDLLMQQLSEKPETTESELHELIRPLLRD
                     HFQPALLARFQTVIYRPLTQSAMRIIVEMKLAQVCERLHCHYGLSTSVDERVYDALTS
                     ACLLPDTGARNVESLLNQQLLPVLSRQLLSYLAARQHPTYLSLTWNDEDGIVIAFDGI
                     NSDYPQESIYGQC"
BASE COUNT         1861 a         1941 c         1443 g         1941 t
ORIGIN      
        1 tattgtcatc ctgtagcgtt aaacaccaga catcataact ttagtatttt ttactgcgtt
       61 atcattcagc tattgaccgt ttttttacgc cagatgaaaa ttagactttc tttttacata
      121 ctcatcatta ttctggctta agtcaccagt taccgtaagg ataagtttta tctctgataa
      181 actccttaat atcttcatca tatccatatg agaaatcact tttagtaact cccttacaca
      241 tcttcatttt atcacccgat actgtagtaa ttttagcatc caactgaggt accaccggat
      301 taccgcgatc tcccagccag actcttacct tcccttcagg tgccagacca ataatcatcg
      361 ttgaacgata ataggtttcg ttccagcggt tgtctgtgta aggcgtaacc atctgctccc
      421 aggtcccttt tttaaaaaaa atcagagttt cataactctt tttatcaatt attgaatccc
      481 agcaaaaacg taacatcaga ggtaatgctt tccccttgtt ataatacgac tcagtcgcgc
      541 ccagatggtc attccagcgc cctacactga cctgactcgg tcttgtggta tcaagatact
      601 gataagtcgt cttataacct tccccgtcca ccatcaatgc ctgagtggcc agcgcaggca
      661 gagcctttgg ataaataaag agaaatttcc attcatcata gggtaatttt ttataacccc
      721 attcagtctt atccgaatca gcatactgtg agctaaccga attacaccca gttaacaatg
      781 ccagtaatgg tgcgataaaa atgattttat ctctggtcat atattgccac cctgtggtgt
      841 taaacaccag acatcataac tttagtattt tttactgcgt tatcattcaa tcgctgctga
      901 tcatccatgt accatacaga acgaatccag cgatcatttg gtttattcgg aaaaacgaac
      961 gccttattac ctttaggacc aaagcgatgg ataaaaatct ctcccgttgt aggatccacc
     1021 cataaattac catcagactc aatattccag ttggcagaac aatgggtgta ttttccgata
     1081 atatacaact cttcctcagt aaaagcctga ggctcttgcc ccaaacgtac cgcttttccc
     1141 tgagcaatgg ctttgtcagc aagaaatatt agttcagatg gaagttgtaa ctcagtattt
     1201 gtttgtcgaa ttggttcaaa caatacccct gcttcctgag cagcatccag catcacacgc
     1261 aggcaaacat tagcccagtc attaggtact gccatacgct caaaaaatac tgcggctccc
     1321 acctgctttt gtatcacacc agcccgacgt tgattgctgt tgttcacacc tacggaacga
     1381 attttggtgg ttagttttcc atgaggcaaa atatgtttta aggctggcag agtcatcaac
     1441 atttttctgg cttgctctgc attacggtat actcgcgtat ccgtttctct ggtatcgtca
     1501 ctgacaattt caaattccgg gcaactcaga aacagacttt cattttcctg cagaggtgaa
     1561 cctaccggat tgtaaccacc tccaatatct gaatgcgccc ctggtaaagc cagttcgggc
     1621 cacatacctt taatactgtt caggctgaaa ttatatcggt attcattcat tgcagtaatc
     1681 tggaaaactt tcttcacgac tgaaggacgc aattccagct ttactcccgg gttgctacgt
     1741 ccattaatat cgaagaaatt actgattcca ccaatagctg cgacagtatc aaaaagcccc
     1801 agaaaacgga cttcaccaga aggtttacca tcataaaaat cgcctctgag ccctttagca
     1861 atagctctgg ctatagcagg atcctgttcc ataacccgat tggcaaagtg ccttgccgcc
     1921 gctgctcccc ggctaaaacc aaaaacatca aactggattt ttgcgataca aaaatcagga
     1981 tttaaattga accccataaa ttctgataac gcctgggtga ttcgttccat tgcctcgtcg
     2041 gttttcgtca ccacaccttc aaatatatcc agaatactgg tgcctaatcc catgccaatg
     2101 actgaatctg cttcaccagc cgcagtccct atcccggaaa tgtaagtctg aatttgtgct
     2161 tcaacctgac tttccaacaa agtctgatct gctacgtaaa gtgaatttag ccagtgaata
     2221 ttggaataat atcctcgata actgccattc gagatactgt tcttgttaat ttcttcaaga
     2281 aagtcctcac agctttgtgc atctttttga tttaccccct gtctggctgc actacactca
     2341 ttaaatttca tcaggcgaga tgctgtattc tcccgattgt ttccggtacc atcgaaaaat
     2401 ataccaatgg ttaaagtcac accccgcgcc ggttttttct ttgcaacaac aactttccag
     2461 tcattatgaa tactggtttt atgggtggca tctgttacgt catatccctg atattttgtc
     2521 attctgtttc tccatgaatt tttaccttcc caaactcaac ttttactact gacggaatgg
     2581 aggtgttgac tggcgctgtt ttaccatttt catcggtgat cccttgtaga atatcacctt
     2641 cagccgttgt taatcgataa ggcgtcgaag gttgcggcac tcccgcttca tcggtaagaa
     2701 taaaaccgcc accaaaccct cgcggcattt caacagacgc aatatcaaga cttgttggtc
     2761 ccatcttctg catattcgtg ctttttaaca ggatctgtcc cgggcatccc agctcaatat
     2821 ttccaccgct tagtttgata taagctccac cacatgccag tatcagttcc tgattagcag
     2881 aaacagtcac tttgccctca gaactttgaa tatcaagatt ctgttgggaa ctcattgaca
     2941 aatcgtcact ttgagcctga atatttaccc gcccttttgc tgctaacagt tgcatcccct
     3001 ggtttctgct caacaggcta acagcacctt ctgctacaac tgtaaatgac tgcccggcac
     3061 taatatcagt gttatctccc gacattatgc ccacgctttc actacccgaa gcaattcgta
     3121 acgcccgtgc actaaccatg ccaatcccat caggagcatg caacagcatt cccggcgcag
     3181 tgagatctgt tagcgatgaa ttcaaccgtt gctgacaact tatatctccc tgagtagcat
     3241 ttgcagcagt tgcggccttc gccatacttc gggccagaga taatgcctgc tccagttggc
     3301 ggatagcttc attcatatcc agcatctttc cttgtgcctg aggctgcata tctgcactga
     3361 taaatattcc ttttcctccc cggatactta tccagtcatc agtcctcagc tcagcgcctt
     3421 ctcccctgag tttccgggag gcattaacgt tatgaccaag attaagttgt gtcttaccgc
     3481 caaactcggt actgatcttg atatgctcct caccgcgttt gtcctccatc cgcaacttgt
     3541 tatttgccgg cgtacggata acattgcgcg taccgttctt atcggtaacg tgatcaacat
     3601 gacgtgaatc atgcagggca tgcgcgatat aggggcgatc cggatcacct tcctcaaagg
     3661 caatggccac ttccgttccc tgaatcagcg gaaaatgaaa accataagca tcaccactgt
     3721 agggtttggc cagacgtacc ggcatgcttt cctgtcccgg atttttatca tcacggtcag
     3781 catcaaattt cacccggtac atacctgatg catcctgcca ggcgtagata tccccttctt
     3841 ttgcactggt cacacgggcc gtcatggtac cggtaacttt cggacgcggc agtaaaggag
     3901 ggcgccagca gataacttca ctgtaaggaa cgcccttcag tacaacctgc agggcatcct
     3961 tgcgacttgc agaaaaaccg acacaggtaa gcaacaccgg attgcgcagc agcgcaggca
     4021 acgtcggcgg aagactgtcg gtgatagtca gaacctgagc aggtgccagc gtggcgtctg
     4081 tactgttgcc ggttatccgc acctgctccg ccagataacg ttcatgacca aggcgggcat
     4141 aaaaattggc cgtctccggc accgggtcga ttttatcccc ccgttccaga tgacggaaat
     4201 ggtagtggta cacctcacca tagttaattc cgtcaccatc accatgcgtc atgtctgccg
     4261 gggcggactg caacagatat tgtgcttcac gatggttata agcctttgtg gtgacactgg
     4321 cctctgccac attgtgcgtc acattcagcc cccacactga atccgctctg ttatcattca
     4381 ttccggacgg actgtttatc gccagcgtct tatcaaacgt cagcgcagcc tggctgtcac
     4441 caaaatgcac tacttccgtc tgcgtgtccg gttgcagggt gaaaaaataa aaaatcccca
     4501 cttcagccag caaacgctga ataaattgca ggtcactttc ctgatactgg ttaatctgtt
     4561 cacgcttcgg atacgttttt ttgagacgga actcaaactc ccagcctttc aggccgtgct
     4621 cctggagaac ctctgtcacc acctccggca ccgatttgtt aacgaaaaat cggtgagaac
     4681 gatgctgcct gtccagtagt ttaataaatg gttccagtat cacctggtat tttgcctggt
     4741 cggcggagcc cgatatacgg cgaaaatctg taatgacacc atgtactact ttctggctca
     4801 cctgctcctt aagccctccc gtacccattg taaatgtggc tgatttacgc agcagcagcc
     4861 cggcatccag gtcgttgtca gtactggtaa aattgatgat gtaataatag gtctgactca
     4921 tctcctccct gccgctgaaa tgctcaacat ccagtggtga cgggcaggag ggaataatta
     4981 aatggtaacg attcagggaa gcagagatag tttttgaaag tgtatctgtc agactcatat
     5041 tgttaactcc atgtctgtac cggtattgaa ctgcaattat ataatgatga ccgttaagct
     5101 ttattttcag agagaattac tgataatcat cccattcatc gtaaatatat gcatatgcaa
     5161 atcgatacat cagataaccg acaaaaaata acgcaacaga gagccctccc cacagatatt
     5221 taaattcagc agatgagaga aaaaagaacc agactgtaaa tacaataccc gacgcaccta
     5281 accccatacc tgcgccacgg ataaatcggg aaagcattat aagtaatgtt ctttttatct
     5341 tcataaacca ccgaaaaaat attcgcatat ccggtcaaac tcagccgaat ttcggtctgc
     5401 gacaatacag gctcgttcaa acgggcgcac aatatcttca acaagaaagt acaaaagatc
     5461 caaatccccc tgagctttca gcttatcatg caacaccgga tttctgtctt tcagaaaacg
     5521 ggatgtgtaa atcgcacgcg atgtctctgc gccaacagat aataacaacc cggcaaaact
     5581 ccctgcaaaa aaagcagcca gccctctgga taccagaacc tctcccagtt taaccccggt
     5641 caattcagca aatgccattc tacctgccag atctcctgaa acgttcttca caatggagcc
     5701 tgttttttcc atattgatcc gggatacaaa gtcttcaaga caaatactga taattttccc
     5761 aataatatca cggctgaaaa tgcctttttc taccagtctg gccattctga atttgtcatt
     5821 ctggttttcc cgcctatgcc tggtatctat aaaatcatat cccatataac cgagactgac
     5881 tggcaaagat atgagtccct tcccgacggc ccccagaaac ccatccccat taatatttac
     5941 ggcctccagc atatcccggg caatttcttt agcactgtcc atatatgctt tcctgcggat
     6001 aatcgctatt gattccatca aacgctatca caataccatc ctcatcattc cacgtcagag
     6061 acaggtacgt cggatgctgt ctggcagcaa gatagctgag caactgccgg ctcagtaccg
     6121 gcagcagttg ctggttcagc agactctcca cattccgggc acccgtatcc ggcagcaggc
     6181 aggcagatgt cagggcatcg tacacacgtt catcaaccga tgtgctcagt ccataatggc
     6241 agtgcaggcg ttcacagacc tgtgcaagct tcatctccac aatgatgcgc atggcagact
     6301 gcgtcagcgg acggtaaatc acggtctgga aacgggcaag cagtgctggc tggaagtggt
     6361 cgcgcagcag tggacggata agctcatgca gctccgattc cgttgtctcc ggcttttcgc
     6421 tcagttgctg catcagaagg tcactgccca gattggaggt catcagaatg accgtattac
     6481 ggaagtcaat ttcacgcccc tcaccgtcgc gcataaagcc ccggtcaaac atctggtaga
     6541 acagattcag cacatcccgg tgggcctttt ccacttcatc cagcagcacc acgctgtaag
     6601 ggcgtttacg cacagcctcc gtcagaatac cgccctgacc gtatcccacg tacccgggcg
     6661 gtgacccctt aagctgggaa accgtatggg gctcctggta ttccgacaga ttgatggtaa
     6721 tcagtgattt ttcgccgccg tacatcacat ctgccagcgc cagtgccgtt tcggtttttc
     6781 ccacaccact gggaccgacc agcaggaaca cgccctgggg gccgttaccg gatgtcagac
     6841 ccgtattcgc cgcacgcaga cgctgcgcaa tggattccag tgccacgtcc tgtccgacca
     6901 cccgtctgcc gatatccttt tccagatgca gcagttccgt ctgttcgtcc ttcatcaggg
     6961 aagataacgg caccccggtc cggtccgcga ccacaccggc aacggtgcgt acgtcaacat
     7021 ccacactgag caggggctga ttctgctgaa tgtcgtgcag tgcctgctgc agatgcagtg
     7081 tctcactctg acgggaaata tcgctgcgac tttcaagtaa ctcaccggtg agttcgcact
     7141 cccgggcgta ctgctgctcc aggtcatcac gtgctgtcat cagctc
//
LOCUS       DAZKGF010000208         7109 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZKGF010000208 DAZKGF010000000
VERSION     DAZKGF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.208"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..448)
                     /locus_tag="ACHOQV_004474"
     CDS             complement(<1..448)
                     /locus_tag="ACHOQV_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP9632744.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KMAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVEKVVMTTE"
     gene            complement(582..2180)
                     /gene="tssA"
                     /locus_tag="ACHOQV_004475"
     CDS             complement(582..2180)
                     /gene="tssA"
                     /locus_tag="ACHOQV_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP9632745.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKNIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADDVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLVELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNSGSS"
     gene            complement(2180..5569)
                     /locus_tag="ACHOQV_004476"
     CDS             complement(2180..5569)
                     /locus_tag="ACHOQV_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFP9632746.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTSPRYVTVQDI
                     RHTLRRQHGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPE
                     NADWLAALRRLRYRPADGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLAQPQHM
                     EPARFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPSIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNSADMLNLQTYLTRVTQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLTAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNARWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLHSEGSRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPETVFELSGTSAFTGNDEDAGDSVEETD"
     gene            complement(5562..6710)
                     /locus_tag="ACHOQV_004477"
     CDS             complement(5562..6710)
                     /locus_tag="ACHOQV_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFP9632747.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGALLFIIRASEY
                     VKALNDFSIWWLALTPPGGWFFLFCLRHWQWSNQMDEHLFFKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREIMDK
                     FCQLPAVLPVNVTLITDQPDSEIRSAFVSAWEVLFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCFGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            complement(6714..6980)
                     /locus_tag="ACHOQV_004478"
     CDS             complement(6714..6980)
                     /locus_tag="ACHOQV_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFP9632748.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            complement(7011..>7109)
                     /locus_tag="ACHOQV_004479"
     CDS             complement(7011..>7109)
                     /locus_tag="ACHOQV_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013035836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFP9632749.1"
                     /translation="MCKGVTKHPDGYVYYGDTPDFIKGKIYPYGNW"
BASE COUNT         1774 a         2049 c         1684 g         1602 t
ORIGIN      
        1 gctccgtagt cataacgacc ttctccactg ccagtggatt cagtggtatg gcccgggtgg
       61 tacggtaacg acagactgta tttccggggc caacagggcg ggaagtcact tccagtccgg
      121 ctggtacggt ttcagccatt ttcatgacgg ataaccgggg agtcagtgcc accaccgaca
      181 gggacgggat agtacgcagg taatgaggcc agagcatact gaccagactt tcagtaagct
      241 ccggcaaatc atcatcgatt ttctggcgca gccgtcccat ggaaaaggcg aaaccttcaa
      301 acaggcgttc cacgcaggga tcaggcgtac ccgctttatc gagatccagc atcgccgccc
      361 gatcgggatg agcctgtgca aacgcctttc cggcttcacg cagataacgc atttcagcgt
      421 caaaataacg cagggttaaa tcgtccatta tcttctcaga ataaagctaa ctgttccgga
      481 gactcgcgct ctgacttcag ccatatttca aacaatcgcc cgaactgaac tgcaggcagg
      541 atttcattaa tcagattaca catgaatata attcagacat ctcatgaact tcccgaattg
      601 cacaacacag cagctctggc agcatcaatg gcgatgagcc cggcaagaag atgttccata
      661 tcaggctgca gacgcaccct gtcactttca gtttttgcgg acttcatacg taacagtttc
      721 aggcggcggg cttttacctc aaataccagt tcaggttccc attgtgacag ggtcagccgg
      781 gttgcacgtt catccagctc cacaagcaga tgcagggcaa ggtcattttt tccagtctgt
      841 tcggcaacac gggccatcaa caggcgcagt agccagttgc tgcggtcaga ctgaataccg
      901 ggacgtgcct gtaaccagct taaagcggct tctgttcctt cattatccgc aatctgcagg
      961 gcttccggct caagagataa aatgtcgtta tctccgggag ctgccgttat cgttacagaa
     1021 ggttcttccg cccagcgttc accacactcc atcacctgct gctgtatcca gctcagcgtc
     1081 acgtcatcgg caaacggcat gccgtcgtta aaagccagtg tctcaagccc cggcaggcgc
     1141 agaagcaatc ctttcaggtc atactgaata atggcagcat aattttcttt tccggtctgc
     1201 aacagcgcct gatgaatata ccactgcagg tccagccaca gatgactggc tccccgggca
     1261 aacatatcgt cacactgttc aagcagtgac aaccagttct gctgcagata caaccgtttc
     1321 agtgacgcac gccggtccgg ccccggtggg gcaatacggg tccgtccatc agcagataac
     1381 ggcggtaact gatgcagcgt gtcatgccgg acgcttttca tcagtcgatg agccgccagc
     1441 cagccctcag gctggtcacg cagatatttt gccagaacac gggcctgtga cagcagttcc
     1501 tgtccggagg tgatggcaga taatgccatg acgctgccgg atgttaccgt cggtgccggc
     1561 gcggcagcct cctgaggcac cactgcatca acaccaccgg ccttcatcag gcgattttca
     1621 agtgcctgat ataacccgtt cagcaccggg gcggaagcct catccgtaaa ggtctgttct
     1681 gccagccaca gcgccccggc aatgcgcagg gtatccgcct tcaccacctc cggatacaga
     1741 gacagactgt ccagtatgcg ggcgctgcat aaccactcca gcgccgcctt cctggcccgt
     1801 gaccgttgtg gatgcaggtg ctctccaaaa cgctgcaaca aaccggtcag cagctccaga
     1861 ccgtcggcca gccctttttc accatcctgc cgcagacggg cccagatata ccaggtcgcc
     1921 acccggatat ttttggttct ggtggtcagt aatttttctg caagctggct gacaattccg
     1981 gcatcggctc cggagagttt attgacctct tcacgcatct gctgaaaatc atcatcgtac
     2041 cccgcatcat cacctgcggg attatctggc gtcacgggag caagccattt atcccacagc
     2101 gcaatacgca cctgagccgg ttcagccaca tcccggggtg tcgtctggca tgcactgagg
     2161 agcgaatgaa tggaagccat cagtcagtct cctccacgga atccccggca tcttcgtcat
     2221 tgcctgtaaa cgctgacgta ccggagagtt cgaaaaccgt ttccggcaga acaaaattgc
     2281 ggagtttcag caatacaagc ggcccttccc ccgcttcagt tcgcagtgtg taattcagca
     2341 tacgaccgtc ctgcgcctgc cagctcaggc tccagccact ggcaacgccc ggtgcggctt
     2401 tccgtcgggc catctcaagc agacgtatca gcccccagct tcccggcagg tctgcatact
     2461 gacgggtacc ggcctgagtg cttacccagc ttaaactggc gccaggtgct tccgtatcag
     2521 ccggccaggt aaatcgcttc cataccggca tctgattaac ataaatcagt ttctgattat
     2581 cggttatcag cgtcgtctgc atcacaccgg cagcagttcc cgggcgcagc tcaaaatgca
     2641 gccccgcgtt acctgtggtg aacgccacat ccgctatttc actcagggta ttgatggctt
     2701 tcagaaacgc agggttgaat gtcagtcccc gggtattaat ggtatcaggt acccagcgac
     2761 tgccttcact gtgcaacaca ccactcaggt tattctgcag gaaacgggca atacgtcccg
     2821 tgtccgtatt caggtatttt gccagcaggg gaagcgaagc atcgctgctg acatttttaa
     2881 acggataacg cccggaaaat gcattattcc agccatccac caccgcagta cgccaccggg
     2941 cattcagact ttctgccgcg ggcgtaagaa cctgctgcca cgcctgctcc atcgggcgaa
     3001 caaagaccgt ctgtccgaag ccataccact cctgtccgag tccggcagcg gtcaggctac
     3061 cgtaatcccg ggtatcggtt aaatcaaccg atttcccctg caatactgtc tgtgccagca
     3121 tctgcatcat ggcctgcggg tcagaagagc cggcaatctg ttgcagacgc agacgaacct
     3181 gtgtcacccg ggtcagatat gtctgcagat tcagcatatc cgcactgttg ttctgattat
     3241 ccatcagtgc aagcaccggc ccgaaagtcg tcgccagagg accgtgaagc cggctttccg
     3301 gtactgccac tggctgtttt tcctgtgaaa gcaggtttct ggcagattta accagtgaat
     3361 cagtaaccgc ttcccggggc tggccagtac gcccctgaac cgccagcgta ttcatcagag
     3421 caaccagggg ggactggcgc acatcagcca tcagcgtcag ttgctctgtc acatcagaca
     3481 gcgtctgagc tttacgcaag tgcagactgt tcagaaaatt cagccaggca ttaccaaaat
     3541 cagcaaaata gcgcgttgtc aggcgctgtc tgagtgcttc cggcgatacc ggtgacggtg
     3601 ccttctgcct gccatccgtc agtacccagt ccatttcttc acgacgctca ttaatcacgg
     3661 tgtctatcga cggcaatacg gcctcttccc aggcctggcg ggtaaacata ccgggaacca
     3721 cttcatccgt aaaaaagagg cgggagacat cagtatcacc ggtcatatcc gtcagacgca
     3781 tatccgcaaa ctgatgtgcc acccgtgcca gcattttctg atacagcatg gcatcgctgt
     3841 tctgtgttcc cagatggcga agcagcagag ttctgctctg gctgaccagc tcttcatcag
     3901 ccgtcagctt ccactgagga tgagtaataa tcccggaggc ataatacgcc agcaacgtcg
     3961 ggccattagc ctgccagaaa acggcactca caccgtcacg ctgtggccat tcccgcatga
     4021 gcgtacgact gaacctggcg ggctccatat gttgcggttg cgccagcatc agatacagtc
     4081 tgagctggtc atatgcggct tttgccattt tcccccgctg cgggctgtcc ggaggaagac
     4141 gaataaatgt cctgagctgc tgctcaagat gagcagcagc ggcatcacgc agtaaaagat
     4201 gtgcattttg tgcataaagg ggcattacga ccgcgagcag gtctgcattc tgattcatac
     4261 cggcccgcag gtaccatggc gccccgtgct cctcccggta ctgaaggcgg gccagggatt
     4321 tctgcagatt cagcaacgcc tgcagacgtt ctgcaggaga cagttgctgg ttctgagccg
     4381 gacgaatctg ttcctgtact tcagcaacca gactccggtt tgccagaaag gaaaccacca
     4441 ttcctgttcc ctgagccatc atcagtatgg ccgccataac ggccagactt ctgcgccagt
     4501 tatgacctgt gcgggaaggc tgtagccgga caggtaattg ctgcactgtc tccggcaacg
     4561 cctcccagcg gttatccatt cgccagcgat gtctgacact tcgttcaccg ccgacagttg
     4621 ccggactgaa aacaataccc gccagcaaca actgacggta tggacggagt aatacggata
     4681 atggctccac cacagcatca atatttcgtc tgaatcgctc tgccagcgac aacaaaaaat
     4741 aataccgagg ggcacagcat atctgctgta tcccctgagc aacaagcccg gggagcatgg
     4801 cctgtagttg tgctttcagt ttgtcagaac tacattctgc tggcagaagg catcccacgg
     4861 gctgagtaat acgccctcgc tcatccggac tctcctgcag tgaccagaca tacagcggca
     4921 gacgccagcc aaggcgctca caacatgaac tgaccgcacg ggaaaccctg tcaagggcat
     4981 cttctgtcag tggtgcggat aatgtttcac tcagtccgga agttacccag acaatgccat
     5041 cagcaggccg gtaacgcaga cggcgtaacg cagccagcca gtctgcgttt tccggctgcg
     5101 aggggtctcc tccccagaga agcaatgtcc cctgctcttc ctgccagaac tgttctgtca
     5161 ggcccggggt cagcaactca acctcagagg cagtacctgt tatcaacaga atgcgtattt
     5221 tacgtcccca gaatctgcca tgctggcgcc gcagagtatg acgaatatcc tgaacagtaa
     5281 catagcgcgg tgacgtctga gccagtcgct cttcatcgtc aggtaacctt tgctccttac
     5341 gggcccgacg tcgatatgtc agttcctgaa catatttttt catcacaggc aggtaacaga
     5401 caaagagcaa ccccagactc agcaatatca gcagtagtat ttgatcccgg cgtgtttctg
     5461 cccccagaaa tgctccatat cgggttatca gccaccagag cgcagccaca ccaataaata
     5521 caatccccgg tcgtccaaag cgaccagaca gacaggccag tttattcatt ctggtttcct
     5581 gatgtaacag tactgataaa atgttcttct ccggatgaga ataaagtcag gagagaatca
     5641 ttaccaaaac agaccagatc ggctgccaga gcagtaagca gccacggagc agcaggtccc
     5701 gggataccgc accatttttc cagtaacata cgtccggacg gttcccatcc ggcaccatac
     5761 tggtttccaa tagtcatcag cggtgcagca atcttttccc agtgataaca gtcccccagt
     5821 acacgagcgg tacgacaggc agctgtctgg gtttcaagga aaagtgtgaa ttcatcatta
     5881 aacttgttga tatccagcga catcgggcgt agcagacggg ctggatgcgg caggttatat
     5941 ttttgcgcaa catcatcgct ggtcaacaaa agtgcagcca gtccgtcaga gtatgcattt
     6001 cccccattaa gctgtattac cagtatcaaa tccacagtca gcactggttg tttcagacgc
     6061 tcatcaaccc accccatgga aaagtcggga gtaacctcga tattgtcagg aacaacacgt
     6121 tggggaaata acacttccca tgcagaaaca aaagcagatc tgatttcaga atcaggctga
     6181 tcggttatca gcgtcacatt tacagggaga acggcaggta actgacaaaa tttatccatt
     6241 atctccctaa gcaagacacg taaagaggct tcaaccttat gcccactgtc agaaagataa
     6301 tctattttct ttacaaggcc gtactgcaga ggcaattcat cacaaagagt agctaccgtt
     6361 attttatcag gcaaataaac acaactggca gtaataacaa gatacctttc ggcccaggat
     6421 tcccactgtt tctgagcata ctccccctct tttttaaaga acaaatgttc atccatctgg
     6481 ttgctccact gccagtgtcg caggcaaaac aggaaaaacc agccccccgg aggagtcaat
     6541 gcaagccacc agatactgaa atcatttaat gctttcacat attctgatgc ccggataata
     6601 aacaacagag caccaacaac agccataaat gcccctgtca taaaccacaa catcattgat
     6661 ggtttatgag gtaattctgt tgataatgat tttgttctgc tccatcccat gggttatcct
     6721 attgcacatt caggtgcact ggatataacc ttacacccac acagacattt acatccatca
     6781 atcacaacag ctcttccatc agatatccat tcaggagagc cctccacaat aggattagta
     6841 ccatgtccgg aaacaggaca actaaccata tctccgacaa gagctacttt tttaccatcg
     6901 acaatcatgg ttgaagatgc cgaaataact ttaccaccat gtgtggtatt atcaccaagc
     6961 aatacaaaac ctttagacat aaaactctca aaaataaatt ttactttgag tcaccagtta
     7021 ccgtaggggt aaattttccc tttgataaaa tctggtgtat caccgtaata aacatatcca
     7081 tctggatgtt tagtaacccc cttacacat
//
LOCUS       DAZKGF010000209         7109 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZKGF010000209 DAZKGF010000000
VERSION     DAZKGF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.209"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            12..608
                     /locus_tag="ACHOQV_004480"
     CDS             12..608
                     /locus_tag="ACHOQV_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9632750.1"
                     /translation="MNSTDLIHLIKHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TTEILATLPSLKKMGNYLISFTRSHRSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            679..1626
                     /locus_tag="ACHOQV_004481"
     CDS             679..1626
                     /locus_tag="ACHOQV_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9632751.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENHFNLIIKNNVEHLVKSEST
                     VLRESICAVIAGKDCYQADTLSRLPGVKIISRFGTGIDNIDLHTARQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLLGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHVIIVLLPFSSELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGREIKNVL"
     gene            complement(1928..3304)
                     /locus_tag="ACHOQV_004482"
     CDS             complement(1928..3304)
                     /locus_tag="ACHOQV_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFP9632752.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LSGQFRNAALRLERLAPQGAETIITPPELPRREMNQPKKSEESPQPVFATRSVQQNDK
                     DASPPVPSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(3304..3732)
                     /gene="tssE"
                     /locus_tag="ACHOQV_004483"
     CDS             complement(3304..3732)
                     /gene="tssE"
                     /locus_tag="ACHOQV_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP9632753.1"
                     /translation="MATPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHSLMRQIEETLLRYEPRLNQIQVELLPQPGPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(3736..4272)
                     /gene="tssJ"
                     /locus_tag="ACHOQV_004484"
     CDS             complement(3736..4272)
                     /gene="tssJ"
                     /locus_tag="ACHOQV_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP9632754.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(4253..5332)
                     /gene="tssG"
                     /locus_tag="ACHOQV_004485"
     CDS             complement(4253..5332)
                     /gene="tssG"
                     /locus_tag="ACHOQV_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP9632755.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNAPLGST
                     DSPATDAVRFRPHPGMGFPVSELKNVERDVGNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(5296..7056)
                     /gene="tssF"
                     /locus_tag="ACHOQV_004486"
     CDS             complement(5296..7056)
                     /gene="tssF"
                     /locus_tag="ACHOQV_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP9632756.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSNFQVLSRPVGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLNADSPVACALHRALTLGTQQFWLRLPGQERRVLDAHFS
                     PMGFDDNDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLENVIWPEGMSGFEIDV
                     VLSENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDSDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLSAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSTGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         1677 a         1853 c         1871 g         1708 t
ORIGIN      
        1 ggaaaataaa aatgaacagt acggatctta tccatctcat taaacatttt atgcgtaacg
       61 aacataaagc agttgaagaa gttattgatt cccccctttc ggaggtcgca aatttaatta
      121 aagtattaca gtcttgtcag gggaaagttg tttttattgg tgtcggtaag tccggtatta
      181 ttgcccgaaa actcgccgca acatttgcca gcactggaac tccctcgttt tttgttcacg
      241 gtactgaagc cgtacacggc gatcttggga tggtggcgaa agatgatgtt gttattctta
      301 tttcaaacag tggtgagacg acagaaatcc tggcaacact acctagtctg aaaaaaatgg
      361 gtaattattt gatctccttt acccgcagtc atcgctcatc gcttgcgata agttgtgatt
      421 tatccgttga aattcccgta aaaagtgagg cggataattt aggtttagcg ccgtcatgct
      481 catcaacagt cgttctggtt gttggtgatg cggtggcgct tgcgctctcc gaattgaaaa
      541 agtttactcg tgctgatttc ggcttatatc atccaggagg tgcgctcggt attaaagcaa
      601 actcataatt ttagaatata aaacagtaac cggtcacgcc atcttttggg gtggcgttca
      661 ttgatgtgag tgtcagctat gaaaaacgtt ctggtcaccg ttcccagctt ttcagcgcgt
      721 tgtgtttctg ccagcaagct gctaagagaa aatcatttta accttattat caagaataat
      781 gtggagcatc tggttaaatc agaatccaca gtattacgag aatctatttg cgcagtcatt
      841 gccggaaaag attgttatca agcggatacg ttatcgcgac taccaggagt gaaaataata
      901 tcgagattcg ggacagggat tgataatatt gatcttcata cggctcgaca atcaggtatt
      961 gtggtcaata atgctgttgg gattaattca aatgcagtcg cggaatttat catcggtctt
     1021 atttttgcga gcatgagaaa tatccccggt agctatcatg caatgcaaaa tggttattgg
     1081 ggtgagtcgc atggctgcga gttacaaggt aaacgtattg gcctgcttgg ctacggcaat
     1141 attggcaaaa cactggcgaa aaggttatct ggttttgatg ttgagctttt agcttttgac
     1201 aaacaacccg actatcaggt tgctgacaaa gctggagttc agtttgtatc aattgaagat
     1261 atctttatgc agtcgcatgt catcattgtt cttttgcctt tttcttctga gctggagaac
     1321 ttcattagtc ataaatattt gtcgatgatg cgcaatggtg ctctgatcat caatgccgca
     1381 aggggaaagt tgctggatga gggcgcttta cttcaggtga ttgaagaacg aaatgtgttt
     1441 gcggcgctgg atgtgtttag ctctgaaccg ttggcgcaat ttagcccact gctacatgcc
     1501 aaaaatatca ttacaacgcc gcatatcgcg gccgccaccg ttgagtcata ccagcaaacc
     1561 gggatacacg ttgcccagtc gataattgat tacttcgcag gaagggagat aaaaaacgtg
     1621 ttgtgagttc agcgaacaaa gattttaaat acaaaaaaac cgcaatttgt agaacacatt
     1681 gcggtttgtc agcattctaa taaattggtt gcgggggcca cagtcaagag gttaatccaa
     1741 tcttttgaaa cttaaggtat tcactgttcc cgaacccaag ctatctaccg aaatatacac
     1801 tcattatctg atgtgaatca acgagtagat cgaatggcct gttcctctct ttttttgtac
     1861 acagagactc cattttaaaa aatggcaagg tcacatatcc cttattggta cataaatccc
     1921 ccgatgttta ctgactttgt tccaccttcg tatccagtaa aatgacatag cgattcaaca
     1981 actgatttaa ccgggtatca atttgcgtca acagcgttgg cgaaacctct ccggtctgtt
     2041 tttgctcttc cagttgccga agcagttctt ccagcggcgg ctcctttaac gactgccgga
     2101 tgccgtacac cgccgttttt agttccgaac cggtcagata tctcccctta cgttcatcca
     2161 gtgcatccag ctgcgccgac aggcgctgta gctggctctg cgcctgagca tagccattca
     2221 gtgcctcagc agacagggcg ctcagctccc gctgtttgcg ccactgcgcg ttcagcgccc
     2281 tgacctgagg attatccggc cacagggcat caagctggcg tagctggcga tatccctgct
     2341 ccagtggcca gcgggcaggc aatttgttta actgctccag ttgcatctga cttgccgcaa
     2401 ttgttctgtc ctgagccagc agcgccttat cctttacatc cagctttgcc agttcgccgc
     2461 tctccagcga ctgcggcaac ggcatgacag acagttgtat tcgctggatt agcgcgtccg
     2521 gctgattaag ctgcgaccag ccccataatc ctgttccgcc gagaccggcc atcacaacca
     2581 ttccggccat aaatatcggc catgtccgct gccgggagat ttcaggggat ggcacgggtg
     2641 gactggcatc cttatcgttt tgctgaacgg atctggttgc aaaaaccggc tgtggacttt
     2701 cctctgactt tttcggttga ttcatttccc ggcggggtaa ttcaggggga gtgataatgg
     2761 tttccgctcc ctgaggcgcc agccgttcca gacgcagagc cgcattgcga aactgcccgg
     2821 acaatacgtc cagcttcgtc atatgccaca gttcacactg ttgcagcgtg gtttcgatgg
     2881 cggaaagatg ctgtacacaa cggtagatct gcggcagatc ctgatactga atatccagcg
     2941 ttctcagtgc ctgctgcatt ttttctgtga cccagctgag cagtgatatt ctggaatgta
     3001 cctgtaccgg ccagaagtca gcccagcgct ggagcagtat atccagtgaa ccgagtccgt
     3061 ctgccatccc tgccagcccc tgccgtctgg ttattgccag cacgtaacag accagcgtct
     3121 gtagttccac gccattttgc cggaacagcg cgaggcagag tttttccacc tgacgccagt
     3181 ccacatccgg tcgggccggg tgagtcagtt tactcatttc agcctgcagt tcaccggatt
     3241 ccttataagt acagggatcg ccgcccatga cgaactggct gataaaattc gcgttacttg
     3301 ccatcagtac gcccgctcct gttgtttgag atgacgcaga acaattcgtc cttccggaga
     3361 aaatacgcca tcaaagcgta tccagccggt atcgggaagc tgcgcgtgga tcaggtaatt
     3421 aagatgcccc ggaccgggct ggggcagtaa ctccacctgt atctgattca ggcgtggttc
     3481 ataacgcagc agcgtttctt caatctggcg catcaggcta tgaatacccg cagccatccc
     3541 ctgatggatc aggctgagat ccggcagacc gtaatccggc aaatgcgcca gtgtgcctgc
     3601 ccgacagttg ataatgcgtt gcatattgtc catcacggaa aggataagct gatcccgctc
     3661 actgacctgt tccagcggta actcgccgct gaaactgaac gtcagcattt catataagga
     3721 aggtgtcgcc atcggttatt tatccttgag aggcagcagt cgcaggtcgc ttctcatcag
     3781 ttcaatcgag cgcggcttgt cggcctccag ttcgtccctt ttgatcacca gacgccagct
     3841 gtcgcttttt tcatccgggt gataaaactg cccaataatg gcgacaaact gcgtctcttt
     3901 atccagcggc acattcaggg atgccgtgcc ttcaggtttt acccagacgg tgtgttttgc
     3961 cagtacgtcc ttctccagcg tcttctcttc ctgcataaac agggtgtcat aatccgcctt
     4021 atcaaaatct tcgcgggttt tcagttgcca gacatgcacc atcgtggaaa gcggcgtatc
     4081 ttccgcatcc gtattcaggg cgctgcggga gaagaaatcg agatgcagta ttttgatttg
     4141 tttgtagaaa acggctgagg ccacgctttt cgattcatcc accactttct gaatcagccc
     4201 gcagccactc agaacaaccg tcagcatcgc catacagagc aacgccggac gtttaaaacg
     4261 aataggaaac atgagtcata ctctccctgt tacgaatgga aaaagcgggt attaatccct
     4321 gataacgccc cagactgacc gtcactgttc cggtcgcggg cgcggcaaag gaggcgcgaa
     4381 ggatgccggt acggctaatc tgtacacgct gttttcccag ccgggcagca ggcaacaggc
     4441 tgacgggcag cgtcagttgc agacgggcct gacagcgcca gcccagataa acgcgcagca
     4501 gcaccagcaa atcgctgtgc agttgcccgc cgggcagcca gccacgtgct tctgcggcat
     4561 cttcggtata cagtttcagc agcaactggc tgttgacgtc ggtaccggta cgccccagca
     4621 gcgttcgcgt tttcaggctg atgcgtgaat tttttgacag acccgcaggg gccgggagaa
     4681 ttacaggctg cggatcgtga ggcaccaccg tcgctttggt cagcggggca agcagcccta
     4741 ccagtgccgt gacgccttcc gccgtgcgcg tcggcaggcg catcacgctg agcagggcca
     4801 gaaaacggga taccggcgtg gcgatatggt tctcgctgcc gggaataccc agcccgatga
     4861 gtcccagcag acaacgggaa atgtcatcca cggcacctgc ttcaaacgac gccgggtagt
     4921 tatatttgcg ccagatgcgg tagtactgag taataaagcg atggttaaaa atatcgagaa
     4981 acgccatcac tgcgtcatgt ccgtcatgac gctgggtaat gtagtccaga taggcggttg
     5041 gcagcggcga atccacgccg tacaggccga gaaaagtggt gcgtaccgtg gggggcgcgt
     5101 cgggattgcc cacgtcacgt tcaacgtttt ttaactcgct gaccggaaag cccatacctg
     5161 gatgaggacg aaagcgcacc gcatcggtgg cggggctgtc ggtactgccc agcggcgcgt
     5221 tctgctggct ctgttccagc aactggcaaa aacggtaaaa attgatccgc gcaatatccg
     5281 gggccagccg gtcggtcagc ccggtacgcg ctggctgtga tgctctgtcc attgcaggca
     5341 ctttcctgtg ggttgcagaa ttaaggtcag ttgagtgaat aagtggatat cggcatacag
     5401 tgcaaagaag cggtgcagca gttcgccaaa cagcgtgata tcaccctctc cggcaaagcc
     5461 ggtgctgtcc agcgtcacct gaatatccac gccacgcagc agaaagcctc tttcaaaacg
     5521 ctgtatcagg ctgtgctgaa cgtcgacaat cgccgacaga cggcggcggt tcatctcgct
     5581 ttcggtccag tcgtacagcg ccagcgtgcc gcgcagaatt tcggcgttat ccatcattga
     5641 aagaaagtta gaaccgaggt ggctgagcac ccgccagtgg aagcggtcac gcgccggggg
     5701 ataacagggc tgcgtcgggg cgcacaggtt gcgcacccgc aggacgttct gtgaggcatg
     5761 aactggcgta tcaagcagcg tgctctgtaa agccttacgg ggaagctgtc cgttagtgcc
     5821 ggtcaggctt aaagagagcg tttcatcctc cagcatccgg tcggaatcaa acgcatcgcc
     5881 gcccagaatc agccaggtat catgcaggcc ggaagggccg cgcttaaccc gcgtatggta
     5941 atagcgttcc ggtgcatcgt ggcgcagcat cccgccccgg tgacgaaagc tggaaaacgg
     6001 gacatacgcc tgactgccgg tatggcgcga agaaataatg ttgtcgacgg aataaatctc
     6061 ggtatgcccg tcctgaatac gcatcgggcg cagcagaaat tcgttttcca gtggcgaaag
     6121 gtgtaacgga tcggcttcca gcgggaacag gttgatgacc ggcacgcagt gcagacggat
     6181 attatcgctg tcaaacggca ggtcgtgagg ccagttctcg ctcagcacca cgtcaatctc
     6241 aaagccgctc atcccttccg gccagatgac gttctccagc ccgttcagcg ccacaaacat
     6301 aaacttttcg cggaaggtga aatactccag cagcaactgg tagccgctga acgccgactc
     6361 gccttttggc cacagtcggt cattgtcatc aaaacccatc ggggaaaagt gcgcgtccag
     6421 cacacggcgt tcctgccccg gcagacgcag ccagaactgc tgggtgccaa gcgttaaagc
     6481 ccggtgcagc gcacaggcca ccgggctgtc ggcattgaga tagagcggca aacgggatag
     6541 atcgatctgt gaccagtcac caaccagcgg gccgcattca aagcgcagac ggataaccga
     6601 gcgcccgtcc ggttcatatt gcaatgacac gtccggcagc gccagcggat gcagcgtgat
     6661 atcccgcgta gcactgtaga cacagcgggt tctgcgctcg ccaacaggac gcgacaatac
     6721 ctgaaaatta ctcagtgttt cgctctgctt catctgccgg tgatctgtcg acagttcaac
     6781 cacggaaagt gacgggatgg ttcgcaggta gtgaggccac agcaggctga ccagcccctc
     6841 ggtcagttcg ggcaggtcgt catccagctt ttcacgcagg cgacccatta aaaaagcgaa
     6901 gccttcgaac aggcgctcca catagggatc gcgtgcgccg ggtttatcaa gattgagcat
     6961 cgcggcgcgg tcggggtaag cctgcgcaaa ttctttcccc gcttcacgca ggtagcgcat
     7021 ttcggcttcg tagtagcgtt gggttagatc gtccatcgga gggattatct tcctttcatt
     7081 gaaagtgaat atcatgcagg tgtagaagt
//
LOCUS       DAZKGF010000210         7099 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZKGF010000210 DAZKGF010000000
VERSION     DAZKGF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.210"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     misc_feature    77..201
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            83..127
                     /gene="pheM"
                     /locus_tag="ACHOQV_004487"
     CDS             83..127
                     /gene="pheM"
                     /locus_tag="ACHOQV_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFP9632757.1"
                     /translation="MNAAIFRFFFYFST"
     gene            411..1394
                     /gene="pheS"
                     /locus_tag="ACHOQV_004488"
     CDS             411..1394
                     /gene="pheS"
                     /locus_tag="ACHOQV_004488"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP9632758.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            1409..3796
                     /gene="pheT"
                     /locus_tag="ACHOQV_004489"
     CDS             1409..3796
                     /gene="pheT"
                     /locus_tag="ACHOQV_004489"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP9632759.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVIECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGAAIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            3801..4100
                     /gene="ihfA"
                     /locus_tag="ACHOQV_004490"
     CDS             3801..4100
                     /gene="ihfA"
                     /locus_tag="ACHOQV_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFP9632760.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            4201..5181
                     /gene="btuC"
                     /locus_tag="ACHOQV_004491"
     CDS             4201..5181
                     /gene="btuC"
                     /locus_tag="ACHOQV_004491"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFP9632761.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGELWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            5244..5795
                     /gene="btuE"
                     /locus_tag="ACHOQV_004492"
     CDS             5244..5795
                     /gene="btuE"
                     /locus_tag="ACHOQV_004492"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFP9632762.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            5795..6544
                     /gene="btuD"
                     /locus_tag="ACHOQV_004493"
     CDS             5795..6544
                     /gene="btuD"
                     /locus_tag="ACHOQV_004493"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFP9632763.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQHQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLFLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            6623..7087
                     /gene="nlpC"
                     /locus_tag="ACHOQV_004494"
     CDS             6623..7087
                     /gene="nlpC"
                     /locus_tag="ACHOQV_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP9632764.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
BASE COUNT         1667 a         1738 c         2006 g         1688 t
ORIGIN      
        1 aacgccatca aaacattgac ttttatcgcc gtagcctttt caataaaggt cttttgaaga
       61 gtaccaaaag gtaacgcaag caatgaatgc tgctattttc cgcttctttt tttactttag
      121 cacctgactc caggaggcta gcgcgtgaga agagaaacgg aaaacagcgc ctgaaagcct
      181 cccagtggag gctttttttg tatgcgcgtt ttgaaaaata aaaagcctgt ctcatcaggc
      241 gtaattgtcg cagccagtct ggacacggcc agcgcggaga aaccggagcg tacttaagta
      301 cgtgaggatt tcgagcactg cccgggacca aaatggcaag taaaatagcc tgatgggatg
      361 ggctctaagt ccaacgaacc agtgtcacca ctgacacaat gaggaaaaac atgtcacatc
      421 tcgcagaact ggttgccagt gcgaaggcgg ccattagcca ggcgtcagat gttgccgcgt
      481 tagataatgt gcgcgtcgaa tatttgggta aaaaagggca cttaaccctt cagatgacga
      541 ccctgcgtga gctgccgcca gaagagcgtc cggcagctgg tgcggttatc aacgaagcga
      601 aagagcaggt tcagcaggcg ctgaatgcgc gtaaagcgga actggaaagc gctgcactga
      661 atgcgcgtct ggcggcggaa acgattgatg tctccctgcc gggtcgtcgc attgaaaacg
      721 gcgggctgca tccggtgacc cgtaccatcg accgtatcga aagtttcttc ggtgagcttg
      781 gctttaccgt ggcaaccggg ccggaaatcg aagacgatta tcataacttc gatgctctga
      841 acattcctgg tcaccacccg gcgcgcgctg accacgacac tttctggttt gacgctaccc
      901 gcctgctgcg tacccagacc tctggcgtac agatccgcac catgaaagct cagcagccac
      961 cgattcgtat catcgcgcct ggccgcgttt atcgtaacga ctacgaccag actcacacgc
     1021 cgatgttcca tcagatggaa ggtctgattg ttgataccaa catcagcttt accaacctga
     1081 aaggcacgct gcacgacttc ctgcgtaact tctttgagga agatttgcag attcgcttcc
     1141 gtccttccta cttcccgttt accgaacctt ctgcagaagt ggacgtcatg ggtaaaaacg
     1201 gtaaatggct ggaagtactg ggctgcggga tggtgcatcc gaacgtgctg cgtaacgttg
     1261 gcatcgaccc ggaagtttac tctggtttcg ccttcgggat ggggatggag cgtctgacca
     1321 tgttgcgtta cggcgtcacc gacctgcgtt cattcttcga aaacgatctg cgtttcctca
     1381 aacagtttaa ataaggcagg aatagattat gaaattcagt gaactgtggt tacgcgaatg
     1441 ggtgaacccg gcgattgata gcgatgcgct ggcaaatcaa atcactatgg cgggcctgga
     1501 agttgacggt gtagaaccgg ttgccggtag cttccacggc gtggtcgttg gtgaagtgat
     1561 tgagtgtgcg cagcatccga acgctgacaa actgcgtgtg acaaaagtga atgtcggcgg
     1621 cgatcgcctg ctggacatcg tctgcggtgc gccaaactgc cgtcagggcc tgcgtgtggc
     1681 ggtagcgacc attggtgctg ttctgccggg cgatttcaaa attaaagcgg cgaaactgcg
     1741 tggcgaaccg tctgaaggga tgctgtgctc cttttctgag ctgggaattt ctgacgatca
     1801 cagcggcatt atcgagctgc ctgcagatgc gccgattggc accgacatcc gcgaatacct
     1861 gaaactcgat gacaacacca tcgaaatcag cgtaacgcca aaccgtgccg attgcttagg
     1921 cattatcggc gtggcgcgtg atgttgccgt gctgaaccag ctgccgctgg ttgaaccgga
     1981 aatcgttccg gttggtgcgg ccatcgacga cacgctgccg attacagtcg aagcgccgga
     2041 agcctgcccg cgctatcttg gccgtgtggt aaaaggcatt aacgttaaag cgccaactcc
     2101 gctgtggatg aaagaaaaac tgcgtcgttg cgggatccgt tctatcgatg cagttgttga
     2161 cgtcaccaac tatgtgctgc tcgaactggg ccagccgatg cacgctttcg ataaagatcg
     2221 cattgaaggc ggcattgtgg tgcggatggc gaaagagggc gaaactctgg tgctgcttga
     2281 tggcactgaa gcgaagctga atgctgacac cctggtcatc gccgaccaca acaaggcgct
     2341 ggcgatgggc ggcatctttg gtggcgaaca ctctggcgtg aatgacgaaa cgcaaaacgt
     2401 cctgctggaa tgcgctttct tcagcccgct gtctatcacc ggtcgtgctc gtcgtcatgg
     2461 cctgcatact gatgcgtctc accgttatga gcgtggcgtt gatccggcac tgcagcacaa
     2521 agcgatggaa cgtgcgaccc gtctgctgat cgacatctgc ggtggtgagg ctggtccggt
     2581 aattgatatc accaacgaag caacgctgcc gaagcgtgcc accattactt tacgtcgcag
     2641 caaactggat cgcctgatcg gccatcatat tgctgatgag caggtaactg acattctgcg
     2701 tcgtctcggc tgcgaagtga ccgaaggcaa agacgaatgg caggccgttg cgccgagctg
     2761 gcgtttcgac atggagattg aagaagatct ggtcgaagaa gtcgcgcgtg tttacggcta
     2821 caacaacatc ccggatgagc cggtacaggc aagcctgatt atgggtactc accgcgaagc
     2881 cgatctgtcg ctcaagcgcg tgaaaacgct gctcaacgac aaaggctatc aggaagtgat
     2941 cacctacagc ttcgttgatc cgaaagtgca gcagatgatc catccaggcg ttgaagcctt
     3001 actgctgcca agccctattt ccgttgaaat gtcagcaatg cgtctttccc tgtggaccgg
     3061 cctgctggca accgtggtgt acaaccagaa ccgtcagcag aaccgtgtgc gcattttcga
     3121 aagtggtctg cgtttcgtac cagatactca ggcaccgttg ggcattcgtc aggatctgat
     3181 gttagccggt gtgatttgcg gtaaccgtta cgaagagcac tggaacctgg caaaagagac
     3241 cgttgatttc tatgatttga aaggcgatct tgaatccgtt ctcgacctga ccggtaaact
     3301 gaatgaggtt gagttccgtg cagaagcgaa tccggcactg catccggggc aatccgcagc
     3361 gatttatctg aaaggtgaac gtattggttt tgttggggtt gttcatcctg aactggaacg
     3421 taaactggat cttaacggtc gcactctggt gttcgaactg gagtggaaca agctcgcaga
     3481 ccgcgtggtg cctcaggcgc gcgagatttc tcgcttcccg gcgaaccgtc gtgacatcgc
     3541 ggtggtggtc gcagaaaacg ttcccgcagc ggatatttta tccgaatgta agaaagttgg
     3601 cgtaaatcag gtagttggcg taaacttatt tgacgtgtac cgcggtaagg gtgttgcgga
     3661 ggggtataag agcctcgcca taagcctgat cctgcaagat accagccgta cactcgaaga
     3721 agaggagatt gccgctaccg tcgccaaatg tgtagaggca ttaaaagagc gattccaggc
     3781 atcattgagg gattgaacct atggcgctta caaaagctga aatgtcagaa tatctgtttg
     3841 ataagcttgg gcttagcaag cgggatgcca aagaactggt tgaactgttt ttcgaagaga
     3901 tccgtcgcgc tctggaaaac ggcgaacagg tgaaactctc tggttttggt aacttcgatc
     3961 tgcgtgataa gaatcaacgc ccgggacgta acccgaaaac gggcgaggat attcccatta
     4021 cagcacggcg cgtggtgacc ttcagacccg ggcagaagtt aaaaagccgg gtcgaaaacg
     4081 cttcgcccaa agacgagtaa tctgatctaa ctaaaaaggc cgctctgcgg ccttttttct
     4141 tttcactgtc gaagagtcac cgtaaaatca acgccatgac acttcagcag aacggatacc
     4201 atgctgacac ttgcccgcca acaacagcga caaaatattc gctggttatt atgcctgtca
     4261 gttttgatgc tgctggcgct tctcttaagc ctttgcgccg gtgaactatg gatctcgcca
     4321 ggtgactggt tttctcctcg tggcgaactg ttcgtctggc aaattcgcct gccacgtacg
     4381 ctggctgtat tgctggttgg tgcggcgctg gctatatccg gcgctgtaat gcaggcgttg
     4441 tttgaaaatc ctctggcaga acctggacta cttggcgtct ctaacggcgc aggcgtgggg
     4501 cttatcgccg cggtattgct tgggcaaggg caactcccca actgggcgct agggctgtgt
     4561 gcgattgctg gcgcgcttat catcacttta atactcttac gtttcgctcg tcgtcatctt
     4621 tcgaccagtc ggttattgct ggctggcgtt gcattaggga ttatctgtag cgcactaatg
     4681 acgtgggcta tctacttttc cacctctgtt gatttacgtc agctgatgta ctggatgatg
     4741 ggcggttttg gcggcgtaga ctggcggcaa agctggctga tgctggcatt gatccccgtg
     4801 ttgttgtgga tctgttgtca gtccaggccg atgaatatgt tagcacttgg cgagatctcg
     4861 gcgcggcaac tgggtttacc cctgtggttc tggcgcaatg tgttggtcgc agcgaccggc
     4921 tggatggttg gcgtcagtgt ggcgctggcg ggtgctatcg gctttattgg tctggtgatc
     4981 ccacatattc tccggctgtg tggtttaacc gatcatcgcg tattacttcc cggctgcgcg
     5041 ctggcagggg cgagcgcatt gctgctggcc gatattgtag cgcgcctggc attagctgcc
     5101 gcagagctgc ctattggcgt ggtcaccgca acgttgggtg cgccggtgtt tatctggtta
     5161 ttgttaaaag caggacgtta gccgcaaaaa gacggtctat gattaaaagc taagattttt
     5221 actgaccaca cccaggagaa acgatgcaag attccattct gacgaccgta gtgaaagata
     5281 tcgacggtga agtgaccacg ctggagaagt acgccggtaa tgtgctgttg attgtcaatg
     5341 tcgcctcaaa gtgtggctta acgccgcaat atgagcagtt ggagaatatt cagaaagcct
     5401 gggccgatcg tggttttgtg gtgctgggat tcccgtgcaa ccagtttctg gaacaagaac
     5461 cgggcagcga tgaagagatt aaaacttact gtaccaccac atggggggtg acgttcccga
     5521 tgttcagtaa gattgaagtt aatggcgaag gacgccatcc gctgtatcaa aaattgattg
     5581 ccgcagcgcc gaccgcagtc gcgccggaag agagcggatt ctatgctcgt atggtcagca
     5641 aaggtcgtgc accgctgtac cctgatgata ttttatggaa ttttgagaaa tttttagttg
     5701 gcagggacgg attggtcatc cagcgttttt ccccggatat gacgccggaa gatcccattg
     5761 tgatggaaag cattaaactg gcgttggcaa aataatgtct attgtgatgc agttacagga
     5821 tgttgcggaa tctacccgcc tggggccgct ttctggcgag gttcgggctg gggagatcct
     5881 gcacctggtg gggccgaatg gcgcgggtaa gagtacctta ctggcgcgaa tggccggaat
     5941 gaccagcggt aagggaagca ttcagttcgc ggggcaacca ctggaagcat ggtccgcaac
     6001 aaaactcgcg ctacatcgcg cctatctttc acaacaccag acgccaccgt ttgcaatgcc
     6061 ggtctggcac tacctgacac tgcatcagca cgataaaacg cgtaccgaac tactgaatga
     6121 tgtcgcaggg gcgctggctc ttgatgacaa actcggacgg agcaccaatc aactttccgg
     6181 cggtgaatgg caacgcgtac gtcttgctgc ggtggtgttg caaatcacac cacaagccaa
     6241 tcccgcaggc caattgctgt ttcttgatga gccgatgaac agtcttgatg ttgcgcaaca
     6301 aagtgcgtta gacaaaattc tgagcgcact gtgtcagcaa ggactggcga ttgtgatgag
     6361 cagtcacgat ctcaaccaca ccttgcgtca tgcgcatcgg gcgtggttgc taaaaggtgg
     6421 aaaaatgctg gccagtggac gcagggaaga ggtgctcacg ccgccaaatc tggcgcaggc
     6481 ctatgggatg aattttcgcc gtctggatat cgaaggtcac agaatgctga tttcgaccat
     6541 ctgatttcat taatatcttg aaaatcgggt aattacgacg ctaaattaat gcaagaataa
     6601 aaaaacagag gaaccgtacg gaatgcgttt ctgccttatt ttgatcacag cactgtttct
     6661 ggccgggtgt agccaccata aagcaccgcc gccaaatgcc agactttctg attcgattac
     6721 cgttattgcc ggtttgaacg accagctaca aagctggcat ggcacgccgt atcgttatgg
     6781 tggcatgacg cggcgcggtg tggactgttc ggggtttgtg gttgtgacga tgcgcgatcg
     6841 tttcgatttg cagctgcccc gtgaaaccaa acaacaagcc tctatcggca cgcaaattga
     6901 taaagacgag ttgctgcctg gtgacctggt ctttttcaaa acgggctccg gacaaaatgg
     6961 tttgcatgta ggtatttatg ataccaacaa ccaatttatc cacgcctcta ccagcaaggg
     7021 agtgatgcgt tcctcacttg ataatgtcta ttggcagaaa aatttctggc aggcgagacg
     7081 aatctagtat gtcgaaaaa
//
LOCUS       DAZKGF010000211         7094 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZKGF010000211 DAZKGF010000000
VERSION     DAZKGF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.211"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            142..1881
                     /locus_tag="ACHOQV_004495"
     CDS             142..1881
                     /locus_tag="ACHOQV_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFP9632765.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNSLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGGGAVANTAATKSDLAAAQLLAPGTADANGTVTYTVSAGLKT
                     STAADVIASLANNAKVNATIANGFGSPTATDYTYNSATGDFTYSATIAAGTNSGDSNS
                     AQLQSFLTPKAGDTANLNVKIGSTSIDVVLASDGKITAKDGSELFIDVDGNLTQNNAG
                     TVKAATLDALTKNWHTTGTPGAVSTVITTEDETTFTLDGGTDATTSGAITVANARMSA
                     ESLQSATKSTGFTVDVGATGTSAGDIKVDSKGIVQQYTGTVFEDAYTKADGSLTTDNT
                     TNLFLQKDGTVTNGSGKAVYVSADGNFTTDAETKAATTADPLKALDEAISSIDKFRSS
                     LGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKA
                     NQVPQQVLSLLQG"
     gene            2200..2919
                     /gene="fliA"
                     /locus_tag="ACHOQV_004496"
     CDS             2200..2919
                     /gene="fliA"
                     /locus_tag="ACHOQV_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFP9632766.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            2965..3516
                     /gene="fliZ"
                     /locus_tag="ACHOQV_004497"
     CDS             2965..3516
                     /gene="fliZ"
                     /locus_tag="ACHOQV_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFP9632767.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            3604..4404
                     /gene="tcyJ"
                     /locus_tag="ACHOQV_004498"
     CDS             3604..4404
                     /gene="tcyJ"
                     /locus_tag="ACHOQV_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9632768.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            4509..5495
                     /gene="dcyD"
                     /locus_tag="ACHOQV_004499"
     CDS             4509..5495
                     /gene="dcyD"
                     /locus_tag="ACHOQV_004499"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFP9632769.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKIGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            5510..6178
                     /gene="tcyL"
                     /locus_tag="ACHOQV_004500"
     CDS             5510..6178
                     /gene="tcyL"
                     /locus_tag="ACHOQV_004500"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFP9632770.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            6175..6927
                     /gene="tcyN"
                     /locus_tag="ACHOQV_004501"
     CDS             6175..6927
                     /gene="tcyN"
                     /locus_tag="ACHOQV_004501"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFP9632771.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
BASE COUNT         1864 a         1704 c         1841 g         1685 t
ORIGIN      
        1 ggcgactaac aaaaaatggc tgtttgtgaa aaaaattcta aaggttgttt tacgacagac
       61 gataacaggg ttgacggcga ttgagccgac gggtggaaac ccaatacgta atcaacgact
      121 tgcaatatag gataacgaat catggcacaa gtcattaata ccaacagcct ctcgctgatc
      181 actcaaaata atatcaacaa gaaccagtct gcgctgtcga gttctatcga gcgtctgtct
      241 tctggcttgc gtattaacag cgcgaaggat gacgccgcag gtcaggcgat tgctaaccgt
      301 tttacttcta acattaaagg tctgactcaa gctgcacgta acgccaacga cggtatctcc
      361 gttgcacaga ccaccgaagg cgcgctgtcc gaaatcaaca acagcttaca gcgtatccgt
      421 gaactgacgg ttcaggcttc taccgggact aactccgatt cggatctgga ctccattcag
      481 gacgaaatca aatcccgtct ggacgaaatt gaccgcgtat ctggccagac ccagttcaac
      541 ggcgtgaacg tactggcgaa agacggttca atgaaaattc aggttggtgc gaatgacggc
      601 cagactatca ctattgatct gaagaaaatt gactctgata cgctggggct gagtgggttt
      661 aatgtgaatg gtggcggggc tgtggctaat actgcagcga ctaaatctga tttggcagca
      721 gctcaactct tggctccagg tactgctgat gctaatggta cagttaccta tactgttagc
      781 gcaggcctga aaacatctac agctgcagat gtaattgcga gtttggctaa taacgcaaaa
      841 gttaatgcca caattgcaaa tggttttgga tcgccaacag ctacagatta tacatacaac
      901 agcgctacag gtgattttac atatagtgca actattgcag ctggtacaaa ttctggtgat
      961 agtaacagtg ctcagttaca atccttcctg acaccaaaag cgggcgatac tgctaactta
     1021 aacgttaaaa ttggttctac gtcaattgac gttgtattgg ctagcgacgg taaaattacc
     1081 gcgaaagatg gttcagaact atttattgac gtagatggta acctcactca aaacaatgct
     1141 gggactgtca aagcagccac tcttgatgca ctgactaaaa actggcatac aacaggcaca
     1201 ccgggtgccg tatctacggt aattacaact gaagatgaaa caaccttcac tctggatggc
     1261 ggtactgatg ctactacttc tggtgcaatc actgtagcaa atgcaagaat gagtgctgag
     1321 tctcttcaat cggcaactaa gtccacagga ttcacagttg atgttggagc tactggtacc
     1381 agcgcaggcg atattaaagt tgatagtaaa ggtatagtac aacaatacac aggtacagtt
     1441 tttgaagacg cttacaccaa agctgatggt tcactgacta ccgataatac aaccaatctg
     1501 tttttgcaaa aagacggaac tgtgaccaat ggttcaggta aagcagtcta tgtttcagcg
     1561 gatggtaatt ttactactga cgctgaaact aaagctgcaa ccaccgccga tccactgaaa
     1621 gctctggacg aagccatcag ctccatcgac aaattccgct cctccctcgg tgcggtgcaa
     1681 aaccgtctgg attccgcggt caccaacctg aacaacacca ctaccaacct gtccgaagcg
     1741 cagtcccgta ttcaggacgc cgactatgcg accgaagtgt ccaacatgtc gaaagcgcag
     1801 atcatccagc aggccggtaa ctccgtgctg gcaaaagcta accaggtacc gcagcaggtt
     1861 ctgtctctgc tgcagggtta atcgtcgtaa cctgattaac tgagactgac ggcaacgcca
     1921 aattgcctga tgcgctgcgc ttatcaggcc tacaaggtga attgcaattt attgaatttg
     1981 cggattttgt aggccggata aggcgtttac gccgcatccg gcaacatgaa tggtaatttg
     2041 tcagcaacgt gcttccccgc caacggcggg gttttttctg cccgcaattt accggtaacc
     2101 cccaaataac ccctcatttc acccactaat cgtccgatta aaaaccctgc agaaacggat
     2161 aatcatgccg ataactcata taacgcaggg ctgtttatcg tgaattcact ctataccgct
     2221 gaaggtgtaa tggataaaca ctcgctgtgg cagcgttatg tcccgctggt gcgtcacgaa
     2281 gcattgcgcc tgcaggttcg actgcccgcg agcgtggaac ttgacgatct gctacaggcg
     2341 ggcggcattg ggttactcaa tgccgtcgaa cgctatgacg ccctacaagg aacggcattt
     2401 acaacttacg cagtgcagcg tatccgtggc gctatgctgg atgaacttcg cagccgtgac
     2461 tgggtgccgc gcagcgtgcg acgcaacgcg cgtgaagtgg cacaggcaat agggcaactg
     2521 gagcaggaac ttggccgcaa cgccacggaa actgaggtag cggaacgttt agggatcgat
     2581 attgccgact atcgccaaat gttgctcgac accaataaca gccagctctt ctcctacgat
     2641 gagtggcgcg aagagcacgg cgatagcatc gaactggtta ctgatgatca tcagcgagaa
     2701 aacccgctac aacaactgct ggacagtaat ctgcgccagc gggtgatgga agctatcgaa
     2761 acgttgccgg agcgcgaaaa actggtatta accctctatt accaggaaga gctgaatctc
     2821 aaagagattg gcgcggtgct ggaggtcggg gaatcgcggg tcagtcagtt acacagccag
     2881 gccattaaac ggttacgcac taaactgggt aagttataac gtcagtaaat gccgcacttt
     2941 aattttgact accaggagtt cttaatgatg gtgcagcacc tgaaaagacg gccattaagc
     3001 cgctatctta aagactttaa acacagccag acccattgcg cgcattgccg taaattactc
     3061 gatcgcatta ccctcgttcg cgacggcaaa atagtgaata aaatcgagat ttcccgcctg
     3121 gacatgctgc ttgatgaaaa tggctggcaa gtggaacaaa aatcatgggc ggcattgtgc
     3181 cgattttgcg gtgatttaca ttgcaaaacg cagagtgatt ttttcgatat tatcggcttt
     3241 aagcaatttc tttttgagca aactgaaatg agcccaggta cggtgcgtga atatgtcgtt
     3301 cgtctgcgcc gtttgggtaa tcatctacac gagcaaaata tttccctcga tcagctgcag
     3361 gacggtttcc ttgatgaaat cctcgccccg tggctgccca ccaccagcac caacaattac
     3421 cgcattgcgt tacggaagta tcaacactat cagcgtcaaa cgtgtaccgg acttgtgcag
     3481 aaatcctcca gtctgcctgc ttctgatata tattaaataa gaataagatg tagcggagtt
     3541 gtttttgtgt ttacaaacaa tggctctaca ctgcaaacag acataacaac attcggggta
     3601 aatatgaaat tagcacatct gggacgtcag gcattgatgg gtgtgatggc cgtggcgctg
     3661 gttgcgggta tgagcgttaa aagttttgca gatgaaggtc tgcttaataa agtcaaagag
     3721 cgcggcacgc tgctggtagg gctggaagga acttatccgc cgttcagttt tcagggggat
     3781 gacggcaaat taaccggttt tgaagtggaa tttgcccaac agctggcaaa acatcttggc
     3841 gttgaggcgt cactaaaacc gactaaatgg gacggtatgc tggcgtcgct ggactctaaa
     3901 cgcattgatg tggtgattaa ccaggtcacc atttctgacg agcgcaagaa aaaatacgat
     3961 ttctctaccc cgtacaccat ttccggtatt caggcgctgg tgaaaaaagg taacgaaggc
     4021 accattaaaa cggctgatga tctgaaaggc aaaaaagtgg gtgtcggtct gggcaccaac
     4081 tatgaagagt ggctgcgtca gaatgttcag ggcgtcgatg tgcgtaccta tgatgatgac
     4141 ccgaccaaat atcaggatct gcgcgtaggc cgtatcgatg cgatcctcgt tgaccgtctg
     4201 gcggcgctgg atctggtgaa gaaaaccaac gatacgctgg cagtaaccgg tgaagcattc
     4261 tcccgtcagg agtctggtgt ggcgctgcgt aaaggaaatg aagacctgct gaaagcggtg
     4321 aatgatgcaa ttgcggaaat gcaaaaagat ggcactctgc aagccctttc cgaaaaatgg
     4381 tttggtgctg atgtgaccaa ataatcagca taatgacaaa aaagggcgct ttcactagcg
     4441 ccttttttat ttacgcgttt tcagcgtgca taataagaaa atcacaacaa cacacatcgg
     4501 aggctgttat gccactgcat aatttaaccc gttttccacg gctggagttt atcggcgcgc
     4561 caacgccgct cgaatatctg ccgcgctttt ctgattatct tggacgggaa attttcatca
     4621 aacgggatga cgtcactccc atggcaatgg gcggcaataa attacgtaag ctggaatttc
     4681 tcgcggcaga tgctctgcgt gaaggtgccg atacgctgat tactgccggg gcgatccagt
     4741 ctaaccatgt gcgccagact gccgcagttg cggcgaaaat cggtctgcac tgcgtggcgc
     4801 tgctggaaaa tcctattggc acaaccgcag aaaactattt aaccaacggc aatcgtttgt
     4861 tgctggatct gttcaatacc cagattgaaa tgtgcgacgc actgaccgat cccaatgccc
     4921 aactggaaga gctggcgacg cgagtcgaag cacaaggctt tcgcccgtat gtcattccgg
     4981 ttggcggttc taatgctttg ggcgcgctgg gttatgtgga gagtgcgctg gaaattgcgc
     5041 aacagtgtga aggggcagtt aatatttcgt cggtggtggt cgcgtcgggc agtgccggaa
     5101 ctcacgccgg actggctgtt gggctggaac acctgatgcc tgaaagcgaa ctgattggcg
     5161 tgaccgtgtc gcgttccgtt gccgatcaat tgccgaaagt ggttaacctc caacaggcga
     5221 ttgcgaaaga actggagctg accgcatcag cggaaatttt actctgggat gactattttg
     5281 cacctggcta cggcgtgccg aacgacgaag gcatggaagc agtgaaattg ctggcgcggc
     5341 tggaaggcat tctgcttgat cctgtgtata ccggaaaagc aatggcgggg ctgattgacg
     5401 gtatcagtca gaaacgcttc aaagatgaag ggccgattct gtttattcat accggcggcg
     5461 cgcctgcgct gttcgcctat catccccacg tttagctccg gaaacgctca tgcaagaaag
     5521 tatacaactg gttattgatt ccttgccgtt cctgctgaaa ggggctgggt atacgctgca
     5581 actcagtatc ggcggcatgt tttttggctt actgctcggg tttattctcg cgctgatgcg
     5641 cctttcgcca atctggcccg tgcgctggct ggcgcgtttt tatatctcta tttttcgtgg
     5701 tacgccactc atcgcacaac tgtttatgat ctattacggc ctgccgcagt ttggcatcga
     5761 attagatccc attccgtcag caatgattgg tctgtcgcta aacactgccg cctatgccgc
     5821 cgaaacactg cgcgccgcca tttcttctat tgataaaggt cagtgggaag ctgctgccag
     5881 tattggtatg acgccgtggc agaccatgcg tcgcgcgatt ttgccacagg cggcgcgtgt
     5941 ggcgttgccg ccgctgtcga acagctttat cagcctggta aaagatacgt cgctggccgc
     6001 gacaatccag gtgccggagc tgttccgtca ggcgcagttg attacctcgc gcacgctgga
     6061 ggtgttcacc atgtacctgg cggcttcgct catctactgg atcatggcga cagtattatc
     6121 gacgttgcag aaccattttg agaatcaact taatcgccag gagagagaac caaaatgagt
     6181 gccattgaag ttaagaacct ggtgaaaaaa ttccacggtc agacggtgct gcacggtatc
     6241 gaccttgagg tgaagcctgg cgaagtggtg gcaattatcg gtccaagtgg ttccggcaaa
     6301 accacgttgc tacgcagcat aaatctgctg gaacaaccgg aagcgggaac catcaccgtt
     6361 ggcgatatca ccattgatac tgcacgttca ttaagtcagc aaaaatcgct gattcgccag
     6421 ttgcgtcagc acgtcgggtt tgtcttccag aactttaatt tgtttccgca tcgtacggtg
     6481 ctggaaaaca ttattgaagg gccggtgatc gtcaaaggcg aaccgaaaga agaggccacg
     6541 gcgcgcgctc gcgagctgct ggcgaaagtt gggctggcag gtaaagaaac cagctatcca
     6601 cgtcgtttgt ctggcggtca acagcagcgt gtcgcgattg cgcgagcgct ggctatgcgt
     6661 cctgaagtga ttttgtttga cgagccaacg tcggcgctgg atccagagct ggtgggcgaa
     6721 gtcctgaaca ccatccgcca gctggcgcag gaaaagcgca cgatggtgat tgtgacgcac
     6781 gaaatgagct ttgcccggga tgttgcggac cgggcgatct ttatggacca ggggcggata
     6841 gtcgagcagg gggccgcaaa agcgttattt gccgatcccg agcagcctcg cacccgccag
     6901 ttcctcgaga agtttctgct gcaataataa gaaaaaatca gccccgacga ttcacctgtc
     6961 ggggcggtgc gccacttcaa acgtgataaa actgcttaac aaatcagcat aactcattaa
     7021 taacataaga gaatgcgatg gcttgcaaaa gtaattcatt gcctgaataa tataaattat
     7081 atataaatct tatt
//
LOCUS       DAZKGF010000212         7078 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZKGF010000212 DAZKGF010000000
VERSION     DAZKGF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.212"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(368..1678)
                     /locus_tag="ACHOQV_004502"
     CDS             complement(368..1678)
                     /locus_tag="ACHOQV_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFP9632772.1"
                     /translation="MSELNQLQKWRREFHHIPESGWCEFITTSKIITILRGMGWQVIT
                     GNKYLSTEYIMGRNATEVADSIKRAVDSGVPHELLDEMDGFTGCVALWDTGKNGAVTA
                     FRFDIDCVQVSESKSKDHIPEKMGFASIHSGLMHACGHDGHISIGLGLAQWVSENNHK
                     LCGKIKLIFQAAEEGVRGAKPIAEGGILDDCDYFLSSHIGMGVPTGNVVASPTDLLCT
                     TKMDFFFYGTPAHAGVNPHLGNNALAAACHCATQLLAIPRHGEGMSRINIGTLNAGEG
                     RNIIPSFARMQLEVRGETETINQYMREHALKIAQGISSIFGLRFEYKVVGEATDLHND
                     PLLVNLIHYIAQEDMNMSLTEKSFGGSEDATLLIRRVQKIGGKAAYIILGSDLKAGHH
                     EVDFDFDENVLDTGVKLFSLCLERLNSPGLNKIVGNGKLESELY"
     gene            complement(1691..2962)
                     /gene="dcuC"
                     /locus_tag="ACHOQV_004503"
     CDS             complement(1691..2962)
                     /gene="dcuC"
                     /locus_tag="ACHOQV_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP9632773.1"
                     /translation="MFNVITGCVILIITVVLLIKRYDTRTTLIGAGILLCIVAMSPLN
                     AFSAFASRMTESGLIQAICTSMGFACVMKFTGCDTCLVHFLTKGLSRLGIFLIPAAVT
                     VSFIVCIAIPSAAGASAAVGATLIPLLISARIHPAIAGAAILSGTIGAYLNPGVAHNS
                     FISKISQTPLMEFVVFHTPTTITCGVICAVILTVVAIVRKETKCDSAGNISEQTQVEF
                     TDRDICFYLKSVAPFLPVALLMLAEFGFLGETKITVPAAMILGCLYALIVSKSNPKEL
                     VSSFFEGMGTSYANILGIIISAAVFIEGLRSVGIIDVFIRFLTDSPEFARWGGTLGPF
                     LMGLITGTGDATAFAFNEVVTPHANNFGYDTNTLGAAVMLASALGRNMSPLAGATIIC
                     AGLAGVNPVEIAKRTAPGMIVTVLFIALLFL"
     gene            3098..4015
                     /locus_tag="ACHOQV_004504"
     CDS             3098..4015
                     /locus_tag="ACHOQV_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005284674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9632774.1"
                     /translation="MKYMPKLQQIKFFNEIVLCGSLRAAARKMNISQPALSRTIKELE
                     NVLGCNLLIRTRDGIVLTEAGKIFSVHSRMILEEIETAALEVAKVNIDNTGKVAFGIS
                     SLFGVTILNNVLDNFRMKYPKKRVMIREGQLSSLIPFLREGVLDFAMGTLTKDMPVGD
                     FIVVELFNAPFTIVARKHHPLRNCTSIDELATARWVFPETQMGYYHYISRLIPFGHPD
                     EPYAPIFTDSVVCIMNLVLYDDYITVLSRARLQEARFKEILCSIPVKYQLPVSQYGLI
                     YPRKRLMTAPAQIMIEYFQQEIQRYDWNG"
     gene            complement(4264..4431)
                     /locus_tag="ACHOQV_004505"
     CDS             complement(4264..4431)
                     /locus_tag="ACHOQV_004505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632775.1"
                     /translation="MHASPVMTMVVCPTVLLCQNGHQKDQYRESLLSQMIASGHKELT
                     RVWQMNEIQAM"
     gene            4580..5413
                     /gene="iolR"
                     /locus_tag="ACHOQV_004506"
     CDS             4580..5413
                     /gene="iolR"
                     /locus_tag="ACHOQV_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator IolR"
                     /protein_id="HFP9632776.1"
                     /translation="MSKHQTQLSLLQDDIRSRYDSLSKRLKQVAQYILDNSNSVAFDT
                     VASIAQHAEVPPSTLIRFANAFGFSGFNEMKQVFRQHLMEETVSYTERARLFRQKNIN
                     DGEPTPEKPGEILKLFSMVNAQVLQQLPMQIKNEQLDAAVSLLSKADNIYVIGLRRSF
                     SVACYLTYALRHLERRAFLIDGIGGMFSEQLSMISSNDVVIAISYSPYAQEVVELVEL
                     GAKRDAHHIVITDSQVSPLAAFSEVCFVIREAQVDGFRSQVASMCLAQTLAVSLALAA
                     E"
     gene            complement(5466..6899)
                     /gene="iolT1"
                     /locus_tag="ACHOQV_004507"
     CDS             complement(5466..6899)
                     /gene="iolT1"
                     /locus_tag="ACHOQV_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol import MFS transporter IolT1"
                     /protein_id="HFP9632777.1"
                     /translation="MPISVNRYNTGYILRICSIAALGGILFGYDTAVISGAISSLTSY
                     FHLSPAETGWAVSSVVVGCVVGSFSSGYLSKRFGRKKSLMISALLFTISAVGTSLSYT
                     FLHFIIYRIVGGLAVGLAATVSPMYMSEVSPKDMRGRALSMQQFAIVFGQILIFYVNY
                     KIASIATNTWLIEIGWRYMFAAGIAPCILFCILVFFIPESPRWMMMAGREEETLKILT
                     KISNEEHARHLLTDIKTSLQNDRLTAHQKLNYHDSNVRFILFLGCMIAMLQQVTGVNV
                     MMYYAPIVLRDVTGSAQEALFQTIWIGVIQLVGSIIGAMIMDKMGRLSLMRKGTIGSI
                     IGLLLTSWALYSQTTGYYALFGMLFFMIFYALSWGVGAWVLISEIFPNRMRSQGMSIS
                     VGFMWIANFMVSQFFPIINENPWLLAHFHGAFPMWIFAACCVFSYFFICRYLPETKGV
                     SLEKMEDIVLTKRRNKPQPIQAERSSD"
BASE COUNT         2142 a         1487 c         1456 g         1993 t
ORIGIN      
        1 ggactacaga gtccagccag tttgctcatc cgtcaattca ggtagatgcg tcaggctttg
       61 tggctggcgg aaaatggctc gttcgctgat attgttgcgt catacaaaaa cagtacgtga
      121 tgagtatgag gcttttttat ctgtatataa tctcttattc tgtaaattag gatagggtaa
      181 tctcgcaatc atcctcttcc catatcacca gtatatgctg agccgtgttt gtctatttgg
      241 ttaaaattcg ctacaatcct attctctctt ttgggaatat aaaagtctcc attttctctt
      301 tattcgttaa tggagactgt atttaaaggc agaaatcagg agtgattgac aggatacctg
      361 tcagcaatca atacaattca gattccagtt ttccgttccc tacaatttta tttagtccgg
      421 gagagttcaa tctctccaga cataacgaaa acagtttaac gcctgtatcc agcacattct
      481 catcaaaatc aaaatcaact tcgtgatgac cagcttttaa atcactgcca agtattatat
      541 atgctgcttt tcctcctatc ttctgtacac ggcgaataag gagcgttgca tcttcactgc
      601 ccccaaatga tttctctgtc aaagacatgt tcatatcttc ttgtgctata tagtgtatca
      661 gattgacaag taaagggtca ttatgtaaat ccgttgcttc accaaccact ttgtactcaa
      721 aacgtaaacc aaatatggaa gaaatacctt gtgctatttt cagagcatgt tcacgcatgt
      781 attgatttat tgtttccgtt tctccacgaa cttcaagttg catccttgca aaagacggga
      841 ttatgttcct tccttcacct gcgtttagcg tgccaatatt tatccgggac ataccttcac
      901 catggcgtgg aatggcaagt aattgcgttg cacagtgaca agcagctgcc agagcattat
      961 ttcccaggtg aggatttaca ccagcatgtg ccggagtacc atagaaaaag aaatccattt
     1021 ttgtcgtaca caacaaatct gtaggtgaag cgacaacatt tcctgtagga actcccatac
     1081 caatatgtga agaaaggaag tagtcgcagt catcaagaat accaccttct gcaataggtt
     1141 ttgcaccgcg aacgccttct tctgcagcct gaaaaattag cttaattttc ccgcataact
     1201 tatggttatt ttcagatacc cattgtgcca atcctaaacc aatcgatata tgaccatcat
     1261 gtccacatgc atgcattaag ccagagtgta tggatgcaaa tcccattttt tcaggaatat
     1321 gatctttcga ttttgattca cttacctgta cacaatcaat gtcaaaacga aacgcagtga
     1381 cagctccatt ttttcctgta tcccataaag caacacaccc tgtaaagcca tccatttcat
     1441 ccagcaattc atggggaacg cctgaatcaa ctgcacgttt tatactatct gcaacttcag
     1501 ttgcattacg gcccataata tactctgttg acaggtattt attccccgta ataacctgcc
     1561 atcccatacc acgcagaata gtaattatct ttgacgttgt gataaattcg caccacccac
     1621 tttccggaat atggtgaaat tctctccgcc atttttgtaa ctgattcagt tcagacatat
     1681 acgcctcctg ttaaaggaat aataatgcaa taaacaatac cgtcacaatc attccggggg
     1741 cagtgcgttt tgcaatttca actggattaa ctccagccag accggcacag ataatagttg
     1801 caccagccaa cggagacatg tttctcccta gcgcagaagc cagcataact gcagcaccca
     1861 gagtatttgt atcgtaacca aaattgttcg catgtggtgt gacaacttcg ttgaatgcga
     1921 atgcagtcgc atcccctgtg ccggttataa gtcccatcag gaatggcccc aatgtaccgc
     1981 cccagcgggc aaattctggt gagtcagtaa gaaatctgat aaacacatca atgataccaa
     2041 cagatcttaa accttcaatg aaaacagctg cggaaattat gatcccaaga atatttgcat
     2101 atgatgtccc cataccctca aagaagctgg aaactaattc tttgggattg gatttactga
     2161 caatcagggc ataaagacaa ccgagaatca ttgcggctgg aacggttatt ttagtctctc
     2221 caagaaaacc aaattcagcc agcataagta atgctacggg aagaaatggt gctacagatt
     2281 tcagataaaa acagatatca cggtcagtga actcaacctg tgtttgttct gatatgtttc
     2341 ctgcagaatc acattttgtt tcttttctta caatagcaac aactgtaaga ataacagcac
     2401 atattacacc acatgttatt gtagtcggag tatggaatac gacgaattcc attaatggtg
     2461 tttgtgatat tttagatata aatgaattat gtgcaacacc gggattgagg taagcaccga
     2521 tagttcctga aaggatagct gctccggcta ttgcaggatg aatacgtgct gaaataagta
     2581 atggtatcag cgttgcacca acagcagctg atgctccagc agcagatgga atcgcgatgc
     2641 aaacaataaa actgacggta accgcagcag gaataagaaa gataccaaga cgagataatc
     2701 ctttcgttag aaaatgaacc aggcatgtat cacaaccagt gaatttcata acacaggcaa
     2761 agcccatact ggtacaaatg gcctgaatga gaccggactc agtcattctg cttgcaaatg
     2821 ccgaaaatgc gtttaaaggt gacatggcaa ctatacatag cagaatacct gcaccaatta
     2881 gtgtcgttcg tgtgtcgtat ctttttatca aaagcacaac tgtgattatc agaatcacac
     2941 atccagttat cacgttaaac atgagatcct ccttcttcaa agaaggataa tctttacttt
     3001 atgtgatttg ctcaaatatt gatcatgaac aaaaaaatca attaaccggg gatactatta
     3061 tgatggaggt gataacagaa aattatcagg cgaaaaaatg aaatatatgc ctaaacttca
     3121 acaaattaaa tttttcaatg aaatagtcct gtgcggaagt ttaagagctg ctgcccgtaa
     3181 aatgaatata tctcagccag cattaagcag aacgataaaa gaactggaga atgtattagg
     3241 atgtaacctt ctgattcgaa caagggatgg tatcgtttta acagaagctg gaaagatatt
     3301 ctcggtccat tcacgtatga ttcttgaaga gattgaaact gcagctcttg aagttgcgaa
     3361 agtaaatata gataacacag gaaaagtagc ttttggaatt tcttcgctgt ttggcgtaac
     3421 aatactgaat aatgtacttg ataacttccg gatgaaatat ccgaaaaagc gtgtgatgat
     3481 aagagaggga cagttgtcct cccttatccc tttcctgaga gaaggtgttc tggattttgc
     3541 aatgggtaca ttaacaaaag acatgccagt tggagatttt attgtggttg aattgtttaa
     3601 cgcaccgttc actatagtgg caagaaaaca ccatccttta aggaactgta catcaatcga
     3661 tgaacttgca actgcaagat gggtattccc ggaaactcaa atgggatact atcattacat
     3721 atcccgtctt attccctttg gacatccgga tgaaccatat gctccaatat ttaccgattc
     3781 ggtagtttgt attatgaatc ttgttctcta tgatgactat ataacggttc tctccagagc
     3841 aaggttacag gaagcaagat tcaaagaaat attgtgttca attcctgtta agtatcaact
     3901 acccgttagc cagtacgggc ttatttatcc acgaaaaagg ctaatgacgg caccagcaca
     3961 gataatgatt gaatattttc aacaagagat acaacgatat gactggaatg gatgagcaat
     4021 acccggaatg atggtgtaca ccgggataac aatttattcg gatgtgccaa tcccacggtt
     4081 gtaatgggca atataattgt tagccgattc gctaatggca gggaaaagcg cggcataaat
     4141 ataagcccac acacatgtaa cgtactgcag atactcgctt ggcagagttg ttacttcttg
     4201 ggaggcagta ccagccatca gatattcccg ttccatgcca aaacctgagt tacccggata
     4261 ccattacatt gcctgtattt cattcatctg ccacacacgt gtcagctctt tgtgacctga
     4321 cgcaatcatc tgactcagca gcgattcacg gtactggtct ttttggtgac cgttctgaca
     4381 gagcaataca gtgggacaaa cgaccatcgt catcactgga gaggcgtgca tggaaactcg
     4441 tcaacaactc aagttcgccg gaacggaacg ttaacaatag aactttactg tataaatttg
     4501 aatactgcta aatgtatgaa tggttcattc atgttaatat tgtgatttgc aaaaatttca
     4561 ttttattaga gtaaaataca tgtctaaaca ccaaacacag ctttccttac tgcaggacga
     4621 catccgcagt cgctatgaca gtctcagcaa acgtcttaaa caagttgctc agtatattct
     4681 ggataacagt aatagcgttg cttttgatac ggttgcttct attgcgcagc acgcggaggt
     4741 acctccttcg acattaattc gttttgccaa tgcgtttggt tttagcggat tcaatgaaat
     4801 gaaacaagta tttcgccagc atttgatgga ggaaaccgtt agctatacag aacgggctcg
     4861 tctgttcaga caaaaaaaca tcaatgatgg ggaacccaca ccggaaaaac cgggcgaaat
     4921 tctgaagcta ttttccatgg taaatgcaca ggtactacaa caattaccga tgcagattaa
     4981 aaatgagcaa ctggatgctg cagtaagttt attgtcaaaa gccgataata tttatgtgat
     5041 tggattacgt cgttcattta gcgtggcctg ttatttaacc tatgcattgc gccatcttga
     5101 gaggcgtgca tttttaattg atggtattgg aggcatgttt tctgagcaac tgagtatgat
     5161 cagctctaac gatgtagtca tcgccatcag ttattcgcca tacgctcagg aggttgttga
     5221 actggttgag ttgggggcaa aacgtgatgc acatcatatc gtcattaccg acagtcaggt
     5281 cagtccgttg gctgcgttca gtgaggtctg ttttgtaata cgtgaagcgc aggtggatgg
     5341 cttccgttcg caggtagcgt cgatgtgtct ggcgcagacg ttggccgtct cactggctct
     5401 ggctgctgaa taagaggaag atgccgataa tctgtttttc atgatcatcg gcattttggc
     5461 taagcttaat ctgaagagcg ttcagcctga ataggttgtg gcttgttcct gcgttttgtc
     5521 agcacgatat cctccatttt ctctagtgag acacctttgg tttccggcag gtagcggcaa
     5581 ataaagaaat agctaaagac acagcaggct gcgaatatcc acattggaaa tgcaccgtgg
     5641 aaatgggcca gtaaccaggg attttcgtta attataggaa agaactgtga aaccataaag
     5701 tttgctatcc acataaatcc tacagaaata ctcattccct gtgaacgcat gcggttgggg
     5761 aatatttctg atatgagcac ccatgctcca acgccccagg aaagtgcata gaaaatcata
     5821 aagaacagca taccaaagag agcataatag ccggttgttt ggctgtacag agcccatgaa
     5881 gttaataata aaccaatgat gctaccaatg gtgccttttc gcattagcga taaacgcccc
     5941 attttgtcca tgatcattgc gccaataatt gatccgacga gttgaattac gccaatccag
     6001 atggtctgaa acagcgcttc ctgggcgcta ccggtaacat ctctcaatac aataggtgcg
     6061 taatacatca taacgttaac gccggttact tgctgtaaca ttgcgatcat gcaaccgagg
     6121 aaaaggataa aacgtacgtt actgtcatgg taattgagct tttggtgggc cgtcagccgg
     6181 tcattttgca gagaggtttt tatatctgtt agcaaatgtc gtgcatgttc ctcgttagat
     6241 atctttgtaa gaatttttag cgtttcttct tcacgaccag ccatcatcat ccagcggggg
     6301 gattccggta taaagaatac taaaatacag aacagaatgc atggggcaat gccggcagca
     6361 aacatataac gccagccaat ctcaatcagc catgtattgg tcgcaatcga ggcgatttta
     6421 tagttgacgt aaaatatgag gatctgacca aaaacgatgg caaactgttg catactcaat
     6481 gccctgccac gcatgtcctt aggtgaaacc tccgacatat acattggaga tactgttgct
     6541 gcgaggccga cagcaagtcc acctacgata cgataaataa taaagtgcag gaaggtgtag
     6601 gaaagagatg ttcccactgc tgagattgtg aacagcaggg cggaaatcat gagtgatttc
     6661 ttgcgaccaa aacgtttgga taagtaacct gagctgaatg atcccaccac acaacctaca
     6721 acgacgctgg aaaccgccca gccggtttcg gccggagata ggtgaaaata actggtgaga
     6781 gaactgattg cacctgaaat aacggcagta tcgtagccaa ataatatccc gcctaatgct
     6841 gcaatagaac agatacgtaa tatatagcct gtattgtaac gattcactga tatgggcata
     6901 gctgattatc tcctgagcag aaatcatcta tctatttgac ctataatgaa aaaattgtgt
     6961 ttggtcattt ccgtagctct gcagttgtac ttcttgcttt accatcggta attacatttc
     7021 aatattctga ttggtaattt tcactaagat caataatgaa acatttattt tgttatgt
//
LOCUS       DAZKGF010000213         7076 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZKGF010000213 DAZKGF010000000
VERSION     DAZKGF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.213"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1084
                     /locus_tag="ACHOQV_004508"
     CDS             <1..1084
                     /locus_tag="ACHOQV_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9632778.1"
                     /translation="PQNNHLQRPPAAVLYADELAKLKQNDHAPCPPGWQLSLPAARAF
                     ILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISGDA
                     GLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITRTP
                     LEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKRRF
                     DFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEKKT
                     SMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRARLR
                     RYFEQRVATHKEAHWQAYYQARHRLP"
     gene            1095..3374
                     /locus_tag="ACHOQV_004509"
     CDS             1095..3374
                     /locus_tag="ACHOQV_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFP9632779.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPYAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREDFMARWIGWAMQQNNGDVLVVCGGWHASALAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWRWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTGISFPAE
                     LTLNRFTPDGLAQSQVLHRLVILEIPGVVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFNQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLVQLSPTQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALQMPLHCPPQDIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            3367..4503
                     /locus_tag="ACHOQV_004510"
     CDS             3367..4503
                     /locus_tag="ACHOQV_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP9632780.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGDSNLTTPEWINSIHTLFPQRVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSDQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            4500..6503
                     /locus_tag="ACHOQV_004511"
     CDS             4500..6503
                     /locus_tag="ACHOQV_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP9632781.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATTQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIDGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGKCLCQLAALTSRLNSAQEIARRDSVGEVPPMPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVEGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEHSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            6628..>7076
                     /locus_tag="ACHOQV_004512"
     CDS             6628..>7076
                     /locus_tag="ACHOQV_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFP9632782.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGF"
BASE COUNT         1632 a         1891 c         1939 g         1614 t
ORIGIN      
        1 accgcagaat aatcatctgc agcgtccgcc tgccgctgtg ttatacgccg atgaactggc
       61 aaaattaaaa caaaatgatc acgcaccttg cccgcccggt tggcagttaa gtttgcctgc
      121 ggcccgcgct tttatccttg gcgatagcgc gcaaaatatt agccgtaaag tcgtgatcag
      181 tccctccgct gtcgaacgca tgttagtcac tctcgctacc gggcgcggtt tgatgttggt
      241 gggggaacct ggcaccgcaa aatcgctcct gtctgaatta ctggcaaccg ctattagcgg
      301 cgatgccggg ttaaccatcc agggcggcgc atctaccacc gaagatcaaa tcaagtacgg
      361 ctggaactac gccctgctta tcaaccatgg tccgtcaacg gaagcgctgg tccctgcccc
      421 gctttaccag ggaatgcgcg acggcaaaat tgtccgtttt gaagagataa cgcgtacacc
      481 gctggaagta caagactgtc tgttaggtat gctttctgac agggtaatga cggtgccgga
      541 actcaccggt gaagccagtc agctctatgc gcgagaaggc tttaatatta ttgccactgc
      601 caatacccgc gatcgcggcg tcaatgagat gagtgccgcg ctaaagcgtc gcttcgattt
      661 tgaaaccgtc tttccgatta tggatttcgc ccaggagctg gaactggtcg ccagtgcttc
      721 ggcgcgtttg ctggcccata gcggtattcc gcataaagtg cctgacgcgg tactggagtt
      781 actggtccgc accttccgcg atctgcgtgc caacggcgaa aagaaaacgt caatggatac
      841 cctgacggca attatgtcca ccgctgaagc cgtgaacgtg gcccacgcag tgggcgtccg
      901 cgcctggttt ttagcgaatc gcgcaggtga gccagccgat ctggtggact gtattgccgg
      961 aaccatcgtc aaagataacg aagaagatcg cgctcgtctg cgccgttact tcgaacagcg
     1021 agtcgccaca cataaagaag ctcactggca ggcttattat caagctcgcc accgcctgcc
     1081 gtgaggaaag atgcatgagc gagccgttaa ttgtcggcat ccggcatcat agtccggcct
     1141 gcgcccggct ggtgaaatcg ttaatcgaaa gccagcggcc acgatacgtg ttgattgaag
     1201 gcccggctga ttttaatgac cgggtagacg aactgttttt agcccaccag cttccggtag
     1261 ctatttacag ttattgccag tatcaggacg gtgcagcccc cgggcgtggt gcctggacgc
     1321 catatgctga attttcgccg gagtggcagg cgctacaagc cgctcgtcgt attcaggcac
     1381 aaacttactt catcgatttg ccttgctggg cgcagagtga agaagaggac gattcgcctg
     1441 atacgcaaga ggaaagccag accttgctgc tgcgtgccac ccgcatggat aacagcgaca
     1501 ccctgtggga tcacttgttc gaagatgaaa gccagcaaac tgcattaccc tctgcactgg
     1561 cgcactattt tgcacaactg cggggcgatt cccccggaga tgctctcaat cgtcagcgcg
     1621 aagactttat ggcccgctgg attggttggg cgatgcagca aaacaatggc gacgtgttag
     1681 tcgtctgcgg tggctggcac gcttctgcac tggcaaaaat gtggcgcgaa tgcccgcagg
     1741 aaattaacac gccggaattg ccctcgctgg cagatgccgt tacaggttgt tatctcaccc
     1801 cctacagtga aaagcgcctt gatgtgctgg caggatacct ttccggtatg cctgccccgg
     1861 tctggcaaaa ctggtgctgg cggtggggct tgcagcaggc aggtgaacaa ctgctgaaaa
     1921 cggttcttac ccgtttgcgc cagcaccact tgcctgcttc aacagcggat atggctgccg
     1981 ctcatctgca tgcgatggca ctggcacagt tgcgtggtca tacactaccg ttacgcactg
     2041 actggctgga tgccatagca ggctcgctga ttaaagaagc cctgaatgcg ccgttgccgt
     2101 ggagctatcg cggcgttatt catcccgata ccgatccgat tctgctaacg ttgatagaca
     2161 cattagcggg tgacggattc ggtaaacttg ccccctctac gccacagccg cctctgccaa
     2221 aagatgtcac ctgcgaactg gaacgtacag gaatctcttt tccggcggag cttactttaa
     2281 atcgctttac ccccgatgga ctggcgcaaa gtcaggtgtt acatcggctg gtaatactgg
     2341 agatcccagg ggttgtgcgc cagcagggaa gtacactgac acttgcaggc aacggtgaag
     2401 aacgctggaa attaacccgc ccgcttagcc aacatgcggc attaattgag gccgcctgct
     2461 ttggtgccac actccaggaa gccgcacgca ataaattaga agccgatatg ctggacgcgg
     2521 gtggaatcgg cagtatcacc acatgtctta gccaggcggc gttagcgggt ctggcgtcct
     2581 tcaatcaaca attactggag caactcacat tattaatcgc ccaggaaaat cagtttgccg
     2641 aaatgggcca ggcgctggaa gtgctatatg ccttatggcg gctggatgaa attagcggta
     2701 tgcagggcgc gcagatatta caaacgacat tatgcgcggc catcgatcgc acgctgtggc
     2761 tatgtgaatc caacggcaga ccggatgaaa aggagtttca cgctcacctg catagctggc
     2821 aagcgctttg ccatatcctg cgcgatctac atagcggcgt taatttaccc ggcgtttctc
     2881 tttctgcggc ggtagcctta ctggagcgac gcagtcaggc gattcatgcc ccggcgctgg
     2941 atcgcggcgc ggcacttggc gcactgatgc gtctggaaca tcccaacgcc agtgccgaag
     3001 cggcgctgac aatgctggtg caattatccc cgacacaatc cggcgaggcg ctgcacggtt
     3061 tgctggcatt agcccgtcat caactggcct gtcagccggt atttatcgcc ggtttcagca
     3121 gtcatttaaa tcaactgagt gatgccgatt ttatcaatgc cctgcccgat ttacgcgcgg
     3181 cgatggcctg gctaccacca cgagaacgcg ggacgctggc gcatcaggtg cttgagcatt
     3241 atcaactggt gcaacttccc gtttcggcac tgcaaatgcc gttgcattgt ccaccacaag
     3301 acattgcaca tcatcaacaa ctcgaacagc aggcactggc atcgctgcaa cactggggag
     3361 ttttccatgt ctgaactgaa cgatcttctg accacccgtg agctacaacg ctggcgatta
     3421 attcttggcg aagcggcaga aacgacgctt tgtgggctgg atgacaacgc ccggcagata
     3481 gaccacgcgc tggagtggct gtatgggcgc gatcctgaac ggctccagcg tggtgaacgc
     3541 tccggtggat taggtgactc aaatctcacc acccctgagt ggatcaacag tattcacacg
     3601 ctgtttccgc aacgggtgat cgagcgactg gaaagcgatg ccgtactgcg ctacggcatt
     3661 gaagatgtgg tgacgaatct cgacgtgctg gaacgtatgc agccttctga aagcctgcta
     3721 cgcgccgttt tgcacaccaa acatctgatg aatcccgaag tgctggctgc cgcccgccga
     3781 atagtgcgcc aggttgttga agaaattatg gcgcgactgg caaaggaagt tcgtcaggct
     3841 ttttctggtg tccgcgatcg ccgccgtcgc tcatttattc cactggcgcg taatttcgat
     3901 ttcaaaagta cgctgcgtgc caacctgcaa cactggcatc cgcaacacgg caagttgtat
     3961 atcgaatccc cccgctttaa cagccgcatt aagcgccata gcgatcaatg gcaactggtc
     4021 ttactggttg atcaaagcgg atcgatggtt gactcggtga tccactcagc ggtgatggcg
     4081 gcctgtttat ggcagttacc cggcattcgt acccatctgg tggcgtttga caccagcgtg
     4141 gttgatctca cggcagacgt tgccgatccg gtagagttat taatgaaagt acaactgggc
     4201 ggcgggacca atatcgccag tgccgtggag tatggtcggc aacttattga acaacctgca
     4261 aaaagtgtca ttatcctcgt gagcgatttc tatgaaggtg gttcatcatc attgctgacg
     4321 catcaggtga aaaagtgtgt ccagagcggc atcaaagtgc tgggactggc ggcgctcgat
     4381 agcaccgcaa caccttgcta tgaccgcgat atggcccagg cactggttaa tgtcggtgca
     4441 caaatagccg ccatgacgcc gggcgaactg gcgacctggc ttgcggagaa tcttcagtca
     4501 tgaattcact acgtccggaa ttattagaac tgacaccaca ggcactgacg gcgttaagca
     4561 atgccggttt tgttaagcgc agccttaaag aactggaaaa tggcaacgtc ccggaaatca
     4621 gtcatgagaa cggcgcttta atcgccacct tcagtgacgg tgtccgcacc cagctggcga
     4681 acggccaggc actgaaagag gctcagtgca gctgcggggc cagcggcatg tgccgccatc
     4741 gcgtgatgct ggttttaagt tatcagcgac tttgcgccac cacccagcct acggaaaaag
     4801 aagaagagtg ggatccggca atctggctgg aagaactggc taccctgccc gatgccaccc
     4861 gcaaacgcgc acaggcgctg gtcgctaaag gcatcaccat tgagttgttc tgtacgccgg
     4921 gcgaaattcc ctctgcccgc ttaccgatga gcgatgtacg tttttattcc cgcagcagca
     4981 ttcgtttcgc tcgctgtgat tgtattgacg gcacactttg cgaacatgtc gtactggctg
     5041 tgcaggcctt cgtcgaggcc aaaactcagc aagcggaatt tactcattta atctggcaga
     5101 tgcgcagcga acacgtcaca tcatctgacg atccgtttgc cagcgaagag ggcaacgcgt
     5161 gtcgtcaata tgttcagcaa ttaagtcagg cattatggct gggcggcatc agccagccgc
     5221 caatccatta cgaggccgct ttcagccgcg cacagcaggc ggcggaacgc tgcaactggc
     5281 gatgggtgag tgaatcgcta cggcaactgc gcgccagcgt tgatgccttc cacgcccgcg
     5341 ccagccacta tcatgccgga aaatgcttgt gccagcttgc ggcgttaacc agtcgcttga
     5401 acagtgcaca agagatagcg cgacgtgaca gtgtcggtga agtaccgccc atgccgtggc
     5461 gcacggtcgt tggctctggc attgccggag aagcaaagct tgatcatctg cggctggtgt
     5521 ctttaggtat gcgttgctgg caggatattg agcattatgg tttacgcatc tggtttaccg
     5581 atcccgacac cggcagtatt ttgcatcttt cgcgtagttg gccgagaagc gaacaggaaa
     5641 actcaccggc agcgacgcgt cggctgttta gttttcaggc tggcgcactg gcgggcgggc
     5701 aaattgtttc acaagcagca aaacgcagtg ccgatggcga gctgctgtta gctacccgca
     5761 accgcttaag cagcgttgtg ccgctgtcgc ctgatgcctg gcaaatgttg agcgcgccgt
     5821 tacgccagcc gggcattgtg gctttgcggg aatatttacg ccagcgtccc cccgcctgca
     5881 tacggcctct taatcaggtc gataacttat ttattctgcc ggtcgctgag tgtatttcgc
     5941 tcggttggga tagcagccgc cagacgctgg atgcgcaggt aatcagcggc gaaggtgaag
     6001 ataatctgct gacgttatca ctgccagcct cagccagcgc gccttatgcc gttgaacgca
     6061 tggcggcgct tttgcaacaa acagacgacc ccgtgtgtct ggtttctggc tttgtcagct
     6121 ttgtagaggg gcaattgaca ctggaaccac aggtaatgat gacaaagact cgtgcctggg
     6181 cgctggacgc agaaactgcg cctgtggtgg catcactacc ttctgccagc gttctgcctg
     6241 tgccgtctac tgctcatcag ttgctgatgc gctgccaggc gttacttatt caactgctcc
     6301 ataacggctg gcgctatcag gaacatagtg ctattggtca ggcagagttg ctggcgaatg
     6361 acctcaccgc ggtgggtttt tatcggctgg cacatgtgtt gggacaattt cgtaatacag
     6421 aaagcgaggc acgggtagaa gcaatgaata acggtgtttt gctgtgcgaa caattattcc
     6481 ccatgcttca gcaacaagga tgaaatagtg ctttttacta agagttctac tccagttccg
     6541 gactgctcac gccacggtat taggcatatc ctatatagcc cctggtgaga gtcaccagtt
     6601 ccttgattaa ataaaatgga gttttacatg aaggctttca ataagctgtt ttccctcgtt
     6661 gttgcatctg ttctggtttt ctctcttgct ggctgcggtg acaaagaaga atcgaaaaaa
     6721 ttcagcgcca atctgaacgg tactgaaatt gccattacct atgtctacaa aggtgacaag
     6781 gtgcttaagc aatcttctga aaccaaaatt caatttgcct ccattggtgc aaccaccaaa
     6841 gaagacgctg ccaggacact tgagccgtta agcgccaaat acaaaaacat cgcgggtgtt
     6901 gaagaaaaat taacctatac cgatacttac gcgcaggaaa acgtgactat cgatatggaa
     6961 aaagtggatt tcaaagccct gcagggtatt tcaggaatca acgtttctgc tgaagatgcc
     7021 aaaaaaggta tcactatggc gcaaatggaa ctggtgatga aagccgctgg ttttaa
//
LOCUS       DAZKGF010000214         7035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZKGF010000214 DAZKGF010000000
VERSION     DAZKGF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.214"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(9..2792)
                     /locus_tag="ACHOQV_004513"
     CDS             complement(9..2792)
                     /locus_tag="ACHOQV_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9632783.1"
                     /translation="MRNLCFLLMLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPSNKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNAQDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLLPLITLYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLETRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2849..5221)
                     /locus_tag="ACHOQV_004514"
     CDS             complement(2849..5221)
                     /locus_tag="ACHOQV_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFP9632784.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDFTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDVNPQ
                     IVFSYIKSKGNLSLNNGYEESNTDDNRVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYINEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5259..6944)
                     /locus_tag="ACHOQV_004515"
     CDS             complement(5259..6944)
                     /locus_tag="ACHOQV_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP9632785.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAEYLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLSVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVI"
BASE COUNT         1827 a         1605 c         1577 g         2026 t
ORIGIN      
        1 ggctgttatt attttggcaa taatactccg gtataggtat tcaccggatg agaaagatat
       61 tgtttaacgg cagtgttaac attctctacc gtcatttgtt tcaataattg ctcctgctca
      121 gtccatgctg caggatcgtc atattgaata agactattta caatggtgtt cgctaattgt
      181 tgaacgctac gctgttggat atcgaggctg cgctgaacgt tttgctggta ttcgttcagt
      241 tcttgctcac tgatcccttt agccagacgc ttaaccatca cttcattcgc taacgttaac
      301 agttcatcat gtcgttccgg ttgacaagta aaagccagca aatgactgat atctttggcc
      361 tgaggatcaa ccgagaggcg agaagaaacg ctgtatgccc cagaggcctg ttcacgaata
      421 ttaacgcgta gatcttttgc cagtgcgacg ttaaaagcat cgagcgccat acgcgtctcc
      481 agattaaccg gtgtccggga atcataacgc ttccactgtg aaacctgtgc cacaggttca
      541 ttttgttctt ttacagtaac cgatgcgttg tccgtcgcgc gagttaatgg tttacctgcg
      601 gctaatggcg aatcagagtg tttgattgat cctaagtaaa gcgtaattaa cggcaggagt
      661 ttgtcttctg cgacattacc gacaatgaca aacgtgatat ccgctggaga tgaaaacaac
      721 tggcgatcgg cagccagcgc atctgcggca gtaaactgtg caatctgatt ttcttgcagt
      781 aatttcgtgc ggtcatcagc atagcgcgtc tcatacatct gctgggcgaa tttctccgct
      841 ggacgctggt cgagcgtttt caacgcctga atttgagcat tttgtagcga tgcccaaata
      901 ttatcgttaa tcgtgctgtg ggtgattcgc tggttaatca actggaaacc aggttcaggg
      961 ttattagtcc gcgcgctaac agagagcaat gtattcatgc cactgacttt actgctcatg
     1021 gttaccgagt tttccgcact ccagcgtttc aggctggaag aggagagttc accaacgccg
     1081 cttccgctta ctgctttatt tgccagcgcg ataagtgatt tttgctgcgc agggaaactt
     1141 aaatcgcctt tattagatac ggcagtaatt tgtagctttt gctcttcacc cgcggatttt
     1201 gccagaataa ccctggcacc attggaaagt gttaatgatg tcagattctc agccagcgtt
     1261 tctttgctgc taatttccgc ctgtgggtca gcgtctactg ttaacgataa atttctgcct
     1321 gggaagatgt aagccgccag ctttttgttg gcgtactctt tttccagcgc caggatagcc
     1381 gcaggagaca atgctttttt ggcggcaact tcattgttta ccatttgctc ccaaaatgcg
     1441 tcctggttct ttcttaactg ctgccatttt tccgccagac tttgcacggt aatttgctgc
     1501 cacagacgtt tcgaaagttg atatgtctct tccggcgaca agaacggcgt attatttaat
     1561 gagctggatg ccaggcgact ggtcagcata cgtaaatcac gctcagcttg ctgatcaacc
     1621 gcatttttca gccaggtaag gcgggtagat ttaacatcat cgagttcttc agcagaaaag
     1681 ccatgctgat caatggttgc caactctgcc attaatgcat tcgcagcatc ctgcatatta
     1741 tcgtcttgtg catttacacg gaaaaacaaa gactgataat cgggtgcaat tttgacgctg
     1801 cgcgcagtgc cgccagaaat agtcttcaac tcgcccgact gtatgcgttc ttgcagacgt
     1861 tggttgaaca gctgaaccaa catgctccat tcagcttgtt cgataaagct ttgctcatcg
     1921 ttcacttgta ccattggcag gcgataatag agtgcgatgc cgttcacccg gttttcttta
     1981 tcattgatga tattaaagcg caggtggttt tcggcttttg tcggccagac gcgattttca
     2041 gctgctttgt tagacggaag cttacttaaa ttatccttaa tcagcgccag cgcttcttta
     2101 ctgtcaatat cgccgaccac gataaaggtc atattatttg gttgatacca gcgttgataa
     2161 aattggcgca attgtgccgg tgtgaccgta gcgacagtat ccatcaggcc gataggttca
     2221 cggtctaaat tacgggtatt tgccagcagg aaagggcggc gcgcctgaga ggtgcgccat
     2281 ttcgcatcct gatgggcacg ccattcctca gtaattacgc cgcgttcagc gtctacttcg
     2341 agtttttcaa aggttgcggc attactccat tcactgaaga ttgccatcac ttgttgcaga
     2401 ttttgtttct gcgtagtcgg caaactcacc tgatacactg tttcgtcata gctggtatag
     2461 gcattaacat cgcgaccaaa acgcaggccc attgactcaa atgtttcgat gactttatta
     2521 cccggccatg tttttgtgcc gttaaacatc atatgttcta caaaatgagc cacgccgcgc
     2581 tcattgtcct cttcctgcaa tgaaccggta tgaatttgca gccataaatt tacctgatcc
     2641 tttggttgag catgcggata aatcatatat cgcaagccat tgtccagttg cccggtaatt
     2701 aacttttcat cctgcggtaa ggcggcggca atcagtcgcc cggggagcaa cagagttgcc
     2761 actaacatca gtaagaaaca gaggtttctc ataattatct ccatgcgaaa accgggcaaa
     2821 tttacccggt aaagtaaaat ccgaactatt aaaatttcat gctgacatca agccagaaag
     2881 tacggccact ggcgtaggtc gccacgccgg tattcgttgt atcaaccgcg gttttgctat
     2941 ccagtacatt gagaatatcg gcactgatcg tcaggttttg ttgtttcaga aattgtggcg
     3001 tccaggacaa gcgggtgtcc caggtcaggc tgctatccag tttctcgtca acatactgct
     3061 tgtaatctga atattcgttg atgtattgcg cattcgtctt acccaggata atgcgagctt
     3121 tacgcgcttc ttgccaggtc agcgtattcg cccacaccaa cccgctcggt tgatgcgtga
     3181 aatccatgtt taaggagatc tttaatggat tattaaaatc cgccactgga acgctatcgt
     3241 aagagaccag attaccgtta taaaccaccc ggttatcatc ggtattgctt tcctcataac
     3301 cattgtttaa cgacaagttg cccttgctct tgatatagct aaagacaatt tgcgggttaa
     3361 catctacctg gctgatatgc aggggttcgg ccagttcaaa actgaggttg aacgaatgcg
     3421 ttttggtttt gccgtcgttg ttatattcag taatggtggt tttagtcgcg ctgtcggtac
     3481 gactgctttt gctgatttga tcatgcgctt cacggtaaac atagtttgcg cgcgcaataa
     3541 cgttcttacc gattttctgc tgcaacccca ttgccagttc atcgttataa ggcgttttca
     3601 aatcctgata acgcgtcagg gttttattgc ctgataccga ttccgtccag ctattgcgga
     3661 tatcacgtaa tcccatatca agaatattcc cgccatagta acggttataa cctgcggtaa
     3721 tcattgaggt ttgatcagca aaaatatccc attccgtcat aaagcgcggg gagatattgt
     3781 ggtttgacag atagttgtca tagtcatacc gcacaccggg cattaacgac acattccgcc
     3841 agctaatgcg atccgccata tacagcgtgt agttgtcaat tcccagactg cctttacctt
     3901 tatggtaaat ggtatggtta gttttctttc ctgcagcatt aatcacatag gattcagact
     3961 ggttatggcg ttcagtccat gcatcggaat agatgtattc cgcgccgaag tagggttgat
     4021 gcgaaacatc acccacggcg aatttttgcc agtccaggcg tgttttgaag gtgtaattat
     4081 ctacagcctg ggaaatgtgt cctaatccac cacgggtgca acgacctgaa atatcaccat
     4141 atgtacatga aagttcggtg taccagatgt catgatcgtg acgggtataa tcactaatat
     4201 gatcccaacc aacggtagta cgtagtttgg cccatgcgag ctgcgtatcc atatcccatg
     4261 ccagaccgta ggatttatta cccatttcgc gatcagactg cgggaaggtg ctggtattat
     4321 aatcacgact ggagccggta tattttaagg taaaatcgtg ggtaaagcgg tcgctggcaa
     4381 accaagtaaa tttgctcaat gcggtatcga taacgttttt atactgtgcc cgaccggcga
     4441 caatgccgtc attcgaaaca taatccgcgc gggtgatatc agactggcgg cgcgataaac
     4501 cggcggtaac gccaaagtta tcagcgagtt cctgattaaa cgacaaggta taaaagttct
     4561 ttttaaaatc tggagagtaa taagtacttc ctgaagaacc ttgattaaat gcgctcttgt
     4621 tattctcatc gatatgcgat gttaaccagt ccgaacgcgt agtgcgataa cccagtttca
     4681 cgctgctatc atcagcgttg aagcgtttga tctttgcatc aattaccccg ccattgaagc
     4741 gaccgaattc aaccggcaca aaactgtcat aaagcgtcac attgtccagt aagctgacat
     4801 caagataata gccctgtgac atcccgctaa tattggttgc actactggca tcggactcat
     4861 tcgctgggtt caggttatta gttgcactaa taccatcaat caaataggca ttctggtagg
     4921 gcgacgcacc gtgaatagag attttctccg ggcgaatatc tccctggttc aatgaggtac
     4981 tttgcgttga atccatgcgc accgcagggt tggttctcag cagatcgctg atattgccat
     5041 tcccggtcgg taaacgctcg atgctttcac tggtgtaatg cgtgttgccg tttacgggta
     5101 cagggacagg agcatagacc gtcatctctt caaaaacatg catgtacatg tttttgtcat
     5161 cgacggcctg cgctacatca gcgccacata aaatgacgcc aggaataaga actcgcttca
     5221 tttatttata tctccgtaca gcagggcttt tattgttttt atataaccgc gctaatgtca
     5281 caaatatcat cagccaggtt ccagacaccg ggttgatgtg taaccataat gacgccgctt
     5341 gtgggtagtt tttcacgcac taaacgcagt aagcggatag cctcttgttc ctcaagatga
     5401 gaggtagttt cgtcaagaaa tatccatttc ggacgtcgta aaattaatcg tgccagggcg
     5461 atacgttgtt tttcgccgct ggaaagaata tcgccccagc gatcgtggtc atgaatacgc
     5521 gcagccaatt tccccagacc aacctgatgc agtacttcgc tcaacgattt atcgtctacg
     5581 gacaggggaa gtgctttaca aattatctct ttcagtaagc cggttttgat taacggtgtt
     5641 tgtgacacat accaactgtc agcaggagaa gaaatatcac ctttaaacca cggccagcag
     5701 tgggataatg ttttaagcag tgtggttttt cccgcaccag agtaaccttt cagtaatagc
     5761 catttgcctg gcgaaacatg aaagttcagg ttctctaata tgatcttatt atcaggagta
     5821 cgaatactcg cgtcagccac ttgtaccgca tgttggcaat ttttaggctt attcgtaggg
     5881 cgctgttcgg tgagttgatg gaactcatac aaacgatcga taaccgcagc cagttcagca
     5941 agttcgtcat atttataaat aaaccagctt aaattgttcg ataccagcat aaatgcctgg
     6001 cgcgatttca tcagtccgcc cagattgatc tgtccgctaa taaactgcgg caataacaga
     6061 aagtagggaa gaacgctaag cgagcgcgaa taaatattct gccagtaatc aagccaccgc
     6121 tgacgattca ttaaacgatg ccagttctct ttaatggtat gaaaattgtc gcttaactcc
     6181 tggcgttgta gatactcagc attgcttagc gcaatcagtt ctgcctgctt attatgctgc
     6241 acaagattag ttcgaaatgt cgcttcgcta cgttgttttt ccacattaag cggacgaata
     6301 cgtttaccaa ccttatgggt aaataaagtt ccaccgatca caatgagcac aacggtatag
     6361 accatatacc cctggatatt ccattctgtt ccaccaacag tgaatgagag cgtacccgcg
     6421 ctttgccaaa gaataacggt aaaggtgatc aacatgctaa gtgactggat gaagccaaat
     6481 gaaaggctga gcgttttact gatcagtaag agaatgtcct cagcgatacg ttggtcaggg
     6541 ttatctgtat ttttatgctc accgtagatc tgcgtgaagt agtagttttt atctgcaaac
     6601 cagcgattaa ggtagtaatc tgttaaccat tcgcgccagc gaatagtcag tagtttaatt
     6661 agccatgttt tgtttacgga tattaataca aagatcccca gcagggcagg gaaccaaagg
     6721 acaagctgcc aaagcttatc ggtttctttc tggcttagcg cattgaaaaa atcattattc
     6781 cagtcgttta accagacctg aattttaacc acgccaagga tcatggcgag gataataatg
     6841 attaacaaca ctgaagtttt attattctta cgcagccaaa atggcttcag cagacagaga
     6901 tactttgcta tcaacataca aaacgtaatg ggaatggtta tcattagcga aaattgattg
     6961 tgccatccaa tgattatcta tggcaatgat ttactgatga aatttattta agtatcattt
     7021 aacaatatga aatat
//
LOCUS       DAZKGF010000215         7022 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZKGF010000215 DAZKGF010000000
VERSION     DAZKGF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.215"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            47..265
                     /gene="yiiE"
                     /locus_tag="ACHOQV_004516"
     CDS             47..265
                     /gene="yiiE"
                     /locus_tag="ACHOQV_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP9632786.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQRGETVVVDDQCKEHKE"
     gene            complement(480..1409)
                     /gene="fdhE"
                     /locus_tag="ACHOQV_004517"
     CDS             complement(480..1409)
                     /gene="fdhE"
                     /locus_tag="ACHOQV_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFP9632787.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1406..2041)
                     /gene="fdoI"
                     /locus_tag="ACHOQV_004518"
     CDS             complement(1406..2041)
                     /gene="fdoI"
                     /locus_tag="ACHOQV_004518"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP9632788.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2038..2940)
                     /gene="fdoH"
                     /locus_tag="ACHOQV_004519"
     CDS             complement(2038..2940)
                     /gene="fdoH"
                     /locus_tag="ACHOQV_004519"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFP9632789.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2953..6003)
                     /gene="fdnG"
                     /locus_tag="ACHOQV_004520"
     CDS             complement(2953..6003)
                     /gene="fdnG"
                     /locus_tag="ACHOQV_004520"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5416..5418),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP9632790.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKVYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTKVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6197..>7022
                     /gene="fdhD"
                     /locus_tag="ACHOQV_004521"
     CDS             6197..>7022
                     /gene="fdhD"
                     /locus_tag="ACHOQV_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFP9632791.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRHDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRL"
BASE COUNT         1571 a         1866 c         1885 g         1700 t
ORIGIN      
        1 aattttcgca catacattaa tgagtaatat atgtaaggta ttggtaatgg cgatgaatac
       61 ggtttttctt catttatcag aagaggcaat taaacggctg aacaagctta ggggctggcg
      121 taaggtttca cgatctgcaa ttttacgcga agcggttgag caatatctgg agcggcagca
      181 atttccggtg cgtaaagcaa agggtggcag gcaaaggggt gagacagtag ttgttgatga
      241 tcagtgtaag gagcataagg aatgacaaaa aatttattat tcttgaatat aaaaaccaga
      301 tgcccttatt ctggtattaa tacaagactg ttttacttga acttataata actgtaactg
      361 ttacatcata tctggaaaac gcctcgcaaa atacgagaga ttttgcggga atagcaggtg
      421 tcgtcacacc ctatgagctg taatccgggc gaccagcgcc acccggaaaa cgtcagagat
      481 tactcccctt cacccgggaa caggaacggg ttgatggaac tgcgggcgta gccttcttgc
      541 tccattcgcg cgtccagtac cagagaggcg aggtcatctg ccacggcttc aactttcggt
      601 tctttttcct gataaagaat cttcaggtaa gtgccacagt catcgcagct ttcggcttta
      661 atcgcggcct gttcgtcatc cagcgaccag taatgcagtt tgccgctctg ctcgcagttg
      721 ctgcatttta cgcgcaccac gtgccattcg gtttcacaca ggttgcaatg caggtaacgc
      781 agaccctgag tggtgccaat ttgcaccatg ctggacaccg gcatagagcc acataccggg
      841 caatattgac gttgttcgcc gtattcagcg cgggctttgc cggggatcag attagccatc
      901 tgcgcccagt agagcgacag cgcagcccag ataaacggcg ctttatcgct gctgacggac
      961 gagaaatcag aggcaaacag tgcgctggcc atatcttcca gctcctgagt cgatgccttc
     1021 tccagattct caatcactgc cagcgccggg ccgctcattt caggtttcag ctcagcaatc
     1081 agcgccatca gcagcttttg ccagtgctta tcacgcggca gaacgtgaat atccagcggg
     1141 ggcttgcctt gtgcgctggc ttctttaatg cgcgtggtca gatccatctc cagcggatgg
     1201 tcgtacagca ccacttcctg ggcgtgggcg ataagcgcag caaagcgcag gtaatcaccc
     1261 agcggattat tttctgccag ctcgcgcaga cgctcggcgc ggcggttgta taaattcttg
     1321 agccgaggga acaataacgg cggaatcata tccgccgtac gtttctcgct cgaacccagc
     1381 tcatcttgcg ggattatgcg aatactcatt cagctttctt ttccgttgtc ttgcggacct
     1441 cacggtacca gcgcgggtga tgtttcttcg cccaggcgct ggtaacccat ccttccacca
     1501 tggcggtaat cgtgcctttc acccaaaggg cggcgtagat atgcaccatg ataaccacaa
     1561 ttaacgccac tgcggcaaat gaatgcagca ttaacgcgaa tcggatcacc gggattgaga
     1621 aagcaggtgc aaaataagga cgccagataa tcacgccgct caccagcagc agaaccagga
     1681 aaataatcgc cgcccagaaa acgcatttct gaccgaagtt ataacgcccg gtgtcaccta
     1741 cttcctcgtt gacgacgatc ttacgaatat tcttcgccca aaagatatca tcccgattga
     1801 ttaggttgtg atgccagtaa cggaaaaaca tgatgatgaa cgaggcaaac ataaccacgc
     1861 cgacaaacgg gtgcagaatg cgcgccagct gcggtgtgcc catgatttgc atcaaccagt
     1921 tgaaggacgg gaacaaaaag cccagcccgc tcaccgccgc caggatgaag cagaaggcgg
     1981 tgatccagtg gttgatacgt tccggcgcgg tgtagcgcac gatggtgtca cgtcgtttca
     2041 tttgcgctcc tcgtctttct cttcgtgcag attattctct tcctcatccg cacggttcgg
     2101 accgacaccc acgtagtgga agatactggc cgcaaaggta gccgcaaagc caacagccgc
     2161 gagcggtttc cagatgcctt tccagaattt cacggtttcg ctgatttccg ggttctccgg
     2221 caagccatga tacagatttg gcttgtcagc atggtgcagc acgtacataa catgtgtacc
     2281 accgacgcct gccgggtcgt acaaacccgc attgtcgtaa ccacgggttt tcagctccgc
     2341 cacgcgctcg ctcgccagcg ttttcatcga ctctttcgta ccgaagtgaa tcgcgcccgt
     2401 tgggcaggtc ttcacgcagg ccggttcttg cccaaccacc acgcggtcaa cgcacaacgt
     2461 acatttgtag acgcggttgt cttccgggtt gaggcgcgga atgtcgaacg gacagcccgc
     2521 aatgcaataa ccgcagccga tgcactgctc ggactggaag tcgacgatac cgttggcata
     2581 ctgaataatt gccccttccg ccgggcacgc tttcaggcag cctggatcgg aacagtgcat
     2641 acagccgtct ttgcggatca gccattccag tttgtcgttc tgctccactt ccgagaatcg
     2701 catcaccgtc cacgatttgg cacttaaatc attggggttg tcgtacaccc caatgttatt
     2761 gccgacggta tcgcgaatgt cgttccactc tgaacacgcc acctgacagg ctttacagcc
     2821 gatacaggtg gtaacgtcga tgagtttcgc cacttcttcc tggaagtccc gcgcctgagg
     2881 cgcgggggtc agaccgttag tcgcggaacg acgaatgata tcttgcgatt gataagccat
     2941 atgtcgtctc cgttacacct tttccacatt cacaaggaag gacttaaact ccggcgtctg
     3001 cgtgttcgca tcaccgacga atggcgtcaa cgtattggca ataaagcctt ttttcgcaac
     3061 accttcatag ccccagtgaa tcggaatacc gatggtatcg atatctttgc cgtttgcttt
     3121 cagcgtgcga atacgtttgg tcactaccgc tttggctttg atatagccac ggttggagga
     3181 gactttcaca gtatcgccct gggcaatgcc aagtttattc gccagcgact ccccgatttc
     3241 cacaaactgc tctggttgca aaatcgcatt caacagcgcg tgtttggtcc agtagtggaa
     3301 gtgctcggtc agacgatagg tggttccgac atacgggaac ttatcggctt tacccaatgc
     3361 ttcggcgtcg tctttaaaga tacgcgcagc cgggttcgag ataacgtttg gatgcagcgg
     3421 gttagttccc agcggcgttt caaacggctc gtagtgttcc gggaacggac cttccgccat
     3481 cttatcgagg gcaaacagac gtcccatgcc ttcctgctgc atgataaacg gccctacgcc
     3541 gctgcccgga ggcgctgcgc tgtagtccgg aatatcccag ccggtccact tagtgccgtc
     3601 ccatttcagc aactgacgct tcggatccca tgggttaccc tgcggatctg cggaggcgcg
     3661 gttatacaga atgcggcggt taagcggcca tgcccatgcc cagcccagcg tgttaccgag
     3721 gccagacgga tcggcgttat cacgacgcgc catctggttg ccttccggcg tccagctacc
     3781 ggcgaaaatc cagcagccac aggaggttgt accgtcatcg cgcagttgag cgaacgagct
     3841 aagttgttgg cctttcttga cgataaccgc accggttgcc ggatcggtaa tatcggccag
     3901 cgccttaccg ttgctctcca tcgccacttc ttctgaagat ggctcatgcg gaatggcgta
     3961 gttccaggtc atgttcagca cctggtccgg gtttgcgcca ccctgttctg catacatctt
     4021 gcgcaagcgc aggaagatac cggagaggat ctcgccatca gtcagcgcaa tccctggggc
     4081 gtccgcacct ttccagtgcc actgtaacca gcgaccggag ttaacgattg aaccgttctc
     4141 ttctgcgaag caagtcgatg gcagacggaa cactttggtc tggatcttcg acgagtcaac
     4201 ttcgttcagc tcaccgtggt tctgccagaa gttagaggtt tcagtgttca gcgggtcgat
     4261 ggtgacgagg aacttcagtt tcgacagaca accgatcact ttgtttttgt tcgggaatga
     4321 ggcaacaggg ttaaagccct ggcagatata gccattgacc ttgccttctt tcatcatctc
     4381 gaagtattgc aggacgtcgt agactttatc ccacttcggc aaccaatcaa agccccagct
     4441 attttccgcc gtcgctttat caccaaagaa ggccttcatc atagagacga agaatttcgg
     4501 gtagttgccc cagtagttta cctggccttc cagcagcggt tttggcgtgt tggcggtaag
     4561 gtaggtttgc agatcggtct gcttctcgct cggcagcgtc atgtaacccg gcaggctctg
     4621 cgacagcagc cccaggtccg tcagcccctg aatattggag tgaccgcgca gggcgttaac
     4681 gccgccgcct gccatcccca tattgccgag cagcaactgg atcatcgcca tcgtacgaat
     4741 gttttgcgca ccaatggagt gttgcgtcca gccgagcgcg taaaggaacg aggcagtttt
     4801 atcgtgagca ctggtctcag cgatgtattc gcagactttc aggaatgcgt cttttggcgt
     4861 accacagatg ttttcaacca catctggcgt gtagcgggaa acgtgctgtt tcagcaagtt
     4921 ccacacgcaa cgtggatgtt gcagggtagt atcgcgtttg gcgaagccgt tttcgtccag
     4981 ttcgtaagtc caggaggatt tatcgtactt acgtttttcc gcgtcatagc cggtgaacag
     5041 gccatcttca aagccgtaat cctcacgcac gatcaggctg gcgttggtat aggcttcggt
     5101 gtattcgcgg ttgaattttt cattgttcag caggtacagc aatacgcctg acaggaaagc
     5161 aatgtcagta ccggaacgaa taggggcata gtagtcagct accgccgccg tacgcgtaaa
     5221 gcgaggatcg atcacaatca gcttcgcgcc attgtgaatt ttggcttcca tcgcccagcg
     5281 gaacccgacc gggtgagctt cagcggcgtt accgcccatc accacgacga ggttggcgtt
     5341 cttgatgtcg acccagtggt tggtcatcgc accgcgacca aatgttggag caagacttgc
     5401 taccgttggt ccgtgtcaga cacgcgcctg gttgtcgacc gcgagcatac ccagcgcgcg
     5461 ggagaatttt tgcgttaaat agccggtttc gttactcgac gcggaagcac acagcatccc
     5521 ggtggagagc cagcggttaa cggtcacgcc ttcggcgttt tgcgcaatgt agttagcatc
     5581 gcggtcttct ttcatcagtt tggcgatgcg atcaaacgcc tcttcccaac tgatttgctg
     5641 ccatttatca gaacctggcg cacggtattc cggaaacttc agacggcttt cggagtggat
     5701 gaaatccacc aggccagcgc ctttcggaca aagtgcaccg cggttgaccg ggtgatccgg
     5761 gtcaccttcg atatggaaga tagatgcttt ggcgtttttt gctccgtcac cgaggctgta
     5821 catcaacagc ccacagccta cggaacaata ggtgcaggta ttacgggttt cgcgggtgcg
     5881 cagcagttta tactgccggg tttccgcgag tgctacgctg ggtgcaaaac ccagtgccgc
     5941 tgccgtggtg cctgccatac cgccagcgca gatcttaaag aactgccttc tgctgacctg
     6001 catggattgc tccttgtttc gacattgtca cgtcccattt acattcgctt gctgcgtgtg
     6061 cagggagtgg gagttatttt tctttgcgga aggggccgca aaggtccaga attggctcaa
     6121 tttccctcca tccaggaagg gtttgtaaca gaataccata atgttggtgt gtgtgttctt
     6181 atctggttaa gagaaagtga aaaaaacaca gcaaaaagaa atcgaaaatg tgacaaatat
     6241 cacaggtgtt cgtcaaattg agttatggcg gcatgatgat ttacaacacc cacggttaga
     6301 tgaggtggcg gaagaagttc ccgttgcgct ggtctacaac ggtatttcgc atgtggtgat
     6361 gatggcgtcg cccaaagacc ttgagtactt tgcgctcggt ttttcgcttt ccgaggggat
     6421 aatcgaaagt ccgcgcgata tcttcggcat ggatgtcgtt ccttcctgta atggtcttga
     6481 agttcaaatt gagctttcca gccgccgctt tatggggttg aaggagcgtc gccgggcact
     6541 ggcgggacgt acgggatgtg gcgtgtgcgg cgtggagcaa cttaatgaca tcggaaaacc
     6601 ggtgcagccg ctaccgttca cccagacgtt tgatctcaac aaactggatg atgcattacg
     6661 tcatctcaat gattttcagc cagtggggca actgaccggt tgtactcacg ccgccgcctg
     6721 gatgttgcca tctggcgaac tggtcggcgg gcatgaagac gtgggtcgcc atgtggcgct
     6781 ggacaaactg ttaggccgcc ggtcacaaga aggggaaagc tggcagcaag gtgcggtact
     6841 ggtttccagc cgtgccagtt atgaaatggt gcaaaagtcg gcgatgtgcg gcgtagagat
     6901 tttgtttgcg gtgtctgccg cgaccacact tgctgtagaa gtggctgaac gctgtaattt
     6961 gacgctggtg ggcttttgta aaccgggtcg ggcaacggtt tatacccatc cgcagcgttt
     7021 ga
//
LOCUS       DAZKGF010000216         6906 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZKGF010000216 DAZKGF010000000
VERSION     DAZKGF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.216"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(276..2561)
                     /locus_tag="ACHOQV_004522"
     CDS             complement(276..2561)
                     /locus_tag="ACHOQV_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9632792.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAITGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELCDAWVDA
                     HGEKESLFTDEAQTHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIRITRTTDWRIVPKVPVVQPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKYGLRTPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEALARGATVNYDG
                     KKFTYPVADEWPGFSTVMARI"
     gene            complement(2551..2826)
                     /locus_tag="ACHOQV_004523"
     CDS             complement(2551..2826)
                     /locus_tag="ACHOQV_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFP9632793.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(2823..3047)
                     /locus_tag="ACHOQV_004524"
     CDS             complement(2823..3047)
                     /locus_tag="ACHOQV_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9632794.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGVSALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRMA"
     gene            complement(3047..3349)
                     /locus_tag="ACHOQV_004525"
     CDS             complement(3047..3349)
                     /locus_tag="ACHOQV_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFP9632795.1"
                     /translation="MSCSHSVVLLNNALKIAVMENGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTEIASYCADEFKKLNDKRSW"
     gene            complement(3349..3573)
                     /locus_tag="ACHOQV_004526"
     CDS             complement(3349..3573)
                     /locus_tag="ACHOQV_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFP9632796.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3637..4137)
                     /locus_tag="ACHOQV_004527"
     CDS             complement(3637..4137)
                     /locus_tag="ACHOQV_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFP9632797.1"
                     /translation="MTVMTLNLVEKQPATMRRIIGKHLAVPRWQETCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHARLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4134..4304)
                     /locus_tag="ACHOQV_004528"
     CDS             complement(4134..4304)
                     /locus_tag="ACHOQV_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632798.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(4315..4590)
                     /locus_tag="ACHOQV_004529"
     CDS             complement(4315..4590)
                     /locus_tag="ACHOQV_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HFP9632799.1"
                     /translation="MNRQLVSVTDAVPYQEFAKLIGKTPRAVRGMIEKGKLPVIEITD
                     PQSVSGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            4712..5011
                     /locus_tag="ACHOQV_004530"
     CDS             4712..5011
                     /locus_tag="ACHOQV_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9632800.1"
                     /translation="MSIDVSEKLKLIRESERLNRKEFSELTGVAYSSLSSYESRSKNA
                     GVEAIMKVLQHPRFTKYTLWFMTDQVAPEAGQIAPALAHFGQNETTSPHSGQKTG"
     gene            5127..6140
                     /locus_tag="ACHOQV_004531"
     CDS             5127..6140
                     /locus_tag="ACHOQV_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9632801.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     ETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPMGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(6417..6734)
                     /gene="yegR"
                     /locus_tag="ACHOQV_004532"
     CDS             complement(6417..6734)
                     /gene="yegR"
                     /locus_tag="ACHOQV_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFP9632802.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAVHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         1839 a         1752 c         1509 g         1806 t
ORIGIN      
        1 gacatcagcc ctgaagcttc aaaactatca tttacgtttg cgaggcacca tctatcacat
       61 tgatttataa taagtatctt tatcttcact gcattgctct caaaaaatca catagatgga
      121 aattccccgt tgacacactc ccaagtgatg cagatacatt tcaagtgctt catacacatg
      181 ataacggcta tccccctaaa ttctgtgcgt ttcttttccc tccagcacaa ggttgaaata
      241 tcttgcgtgc agctttactt tgtgaatctt tgtcattata tccgcgccat tactgttgag
      301 aatcccggcc actcatcagc gaccgggtac gtgaattttt tcccgtcata atttacggtc
      361 gcgccacgcg ccagcgcctc aagctcccat cgctgcggcc tgataccgtt ctgagcaagg
      421 tcaacgcgga tacgggtaat ttgcattctt tccgaccggg tcagtctggc cgatggtgca
      481 atttcatgtg gttttaacgg gcttccgttt ctctgctgac gatttggggt tctcagcccg
      541 tattttaatg cgcccctgag cgccctcacg acctccgggt cattccattc gataacaccg
      601 tcatcaacca gattaagcac tgctgcggcg tgttcagaag gtgtgggagc cggtaacgaa
      661 gtatcaccac cggtgagctt tccacagtta ttgacaggac tccgaggcgc ggcgatgccg
      721 ctttttaaag tcaaaggctg aacgaccgga actttcggca caatgcgcca gtccgtcgtt
      781 ctggtgatac gaatatgacg cgcgccgaga tgcggcgcgt aaatgccgac cactctctcg
      841 acctcttcct cgtactcgtt aacgtcatcc gaggggctac gggcgaccct gacagtctgg
      901 caatcgcgcg ggacatttgc cccaccctgc gcgctgatat acagcgcaaa atcgccactg
      961 tctgcggcag cgcgtgcagc ctcgacgcgt tcgtcaaact catcagcaat gctgacgccg
     1021 cgaggcaatt tgcgtagttc acggtaagcc cccattgtcg gcaggccaac cgttttaaat
     1081 tgaggaatgc gccacgttga cgcccatgcg gtaacagccg cggcagtgtc tttcagcggc
     1141 ctgccggtgt cgttatcgag ctgaccatcc agtgcatagc catcgatgtt ttttgaaatg
     1201 tatttcgcga tatatcccgc agcaccgccc cggttaaggt gttttgcctg aaaacggttt
     1261 cgcgcggctc ctctttcgtc gccatcctct ttgagcgcgt agcgacgcat gatttcgata
     1321 atctgattac gctggcgtgg attacaaaaa agcatcatat gccagtgcgg cgttccgtcg
     1381 tggtgtggct cgacgacacg caaaccgtag acctgtaaat cattatcctt gaatgccgtg
     1441 cgcatcaggc tccagatgcg gcagagataa cgctgcgcat cctttggatt aaatgcctca
     1501 tcgttccagc cgtgattaag ctgcacggtt ttactttcgc cttttccgac ctgacgtgtc
     1561 gggtgatact ttgacggtgc ggtcagcgtg ataaacatcc ccacatcgcc ctctgcggcg
     1621 gcgtaacgct caataccggc aatggtgttc atcagctcca tccggcgaat ttcaggatta
     1681 gaaatactgc ccatcacctt actgataagg tcgatgcgct cgccggtttc cctgttttcg
     1741 aggtcacacg atttaagaaa ttccagattt gcctggcggc gcgcacgcac atcacgaatg
     1801 gcatgtttac tggcataagg agaacggtct ttattgacct ccccgacagc aatcagtaac
     1861 gcctcatgcc agcgcatacg ctggccttta agctgactaa tccaccactc atcgttaaac
     1921 agacgggcaa tggcagaata tgcctgcctc gtggtcatct gccctttacg gtattttttc
     1981 cagtagagcg gggaaatatt gaaagcacgt gcagcgccag cgacatgacc atacaggtga
     2041 gtctgcgcct catccgtaaa cagcgattct ttttcgccat gtgcatccac ccaggcatcg
     2101 cagagttcct catacatcat gaaaagctgc gatgagatac gggcggcaaa ctttttcagc
     2161 tccttgtcat tcattccagg caggcgcgca taatggtcac gctctgccag aaacagcaac
     2221 gacgcgtcgg tgttcatttc atggcgctga ttcacgcgct caatgcgcgg ccataaacga
     2281 cgctgaaaag tggatgtgag gaaataaaac ccgtgcaccg ggcttttatt gcgccggatg
     2341 tagtcataac gtgaagtaaa cagcgagcgc aaaaagtaag gcaggcggtt aatcgtggat
     2401 aaaacacctt gcacctgacg catctcgtca cgtgtaaggg gtctttcgcg cccgacagcc
     2461 tcgcgtggcg cgttccatgc ataagcaccg gtaatcgcct taccggtgcc tgcggcaaat
     2521 gctgacggag ggacaaaacg cccggaggct ttaacggcca tatgagccaa aagcctcaga
     2581 acaacgcttg ctgagttgct caacctgcgc gtttaaatca gcaaaagact ttgcgcttcc
     2641 ggtcagaata tcgtgatgca tcaggccgga aacgagctgg cttaatttcg ggtaataacc
     2701 aaccaccgcc agccattcct gcccggcgtt tttaccgctt tccgctctct ttttctcgtg
     2761 gagaataaac tgaaagctgt cactggtaac gacataacgt tcgccaattt cgatacgaat
     2821 actcatgcca ttctccggta atgcttgttt tttgcttcaa agactgactg gcaggaaaca
     2881 caacgcgtgg ctgacggata agctgcacga cgggcagcag gtattggcgc gtcacactct
     2941 tcgcaaacca gcgcagaaac accgcaatgt tttacccttg ccgcgttaat ctggcgctcc
     3001 agtaattcag cctgttgttc ctgaataaaa tctatgttgt ccggcattac cagctccttt
     3061 tgtcgttcag cttcttaaat tcatcagcgc aatagctggc gatttctgtc gttaattttg
     3121 tcagttcatc cacggatgaa atttgcttgt ggaatacagc acgtttaaca agtaaattga
     3181 ccacatcaga caggaggttt aattcattct gataaatcgc gataacagat tcagttatgt
     3241 cgcgcttttc tttatcaaga caaagttgaa taagagacaa atcaccattt tccataacgg
     3301 cgatttttaa ggcgttattc agtaatacaa ctgaatgaga acaggacatc aaagtacctc
     3361 cccgcgagac aatccgatat tgtgaaattt ttccgactcc tgactgagca gctcgactat
     3421 ctccacgcgg gataactccg cctttgtgat gtggcgaatc atggcgtcaa gatgagaaga
     3481 aaagcgcgta gctgcatctg cctgtgcttc ggttctggcc tgttgcagca gtaatgcgta
     3541 tttaccgcac tgattttcag aaactgtatg catgactttc tccaggcaaa aagaagcccc
     3601 gcacaattaa gtgcgttaaa aactctggtt aattacttaa tgcagatatt gctctggttt
     3661 taccgatgtc agaattgtcg gtgcatactc aaacagactg aataattcac gtaatgcacg
     3721 gaataaagca tcacgccagt aacatgattc ttcattaatt cgccagtacg gctggttgaa
     3781 ttctttttct gtcagtcgtg cgtgcataaa taaagtacga cgctggctga ctgttaaaaa
     3841 actaatatat gcatactcac ttgcaccaac ctgacggcgt tttgagaatg ccccacgcaa
     3901 ttcatcaatt gcacatacca gtcgttcacg ttcgacgtcg ttcatttctt caaaacgcat
     3961 cgttgcgtga cgctgtttta actgcgcatg aaagcaaacc gttagccgtt cgcgttccat
     4021 catctgatta taataatcgc atgtctcctg ccagcgaggg acggccagat gcttaccaat
     4081 tatccggcgc atagttgctg gctgtttttc aacgagattg agcgtcatca ctgtcatttc
     4141 cataccctcc ggcttttcag aaaggtcaga gcctttttta acggactctg ttttttggtg
     4201 cggataatga ttcccttacg ccccttaccg tgggtgatgg tgaagtcaat cgccctgggg
     4261 ctttcgttac gcaataactg agcaatacaa cgaggctcat tcatacggtt ctccttaacg
     4321 tggttcaccg agacctaacc acatcaacca gccgtcacga atctctttag gacggctttc
     4381 ataagccagt tttagtccgt tattccatgc cggaaggtat acccaatatt caccagcacg
     4441 ccccgatact gactgagggt cagtaatctc aataactggt aatttccctt tctcaatcat
     4501 accccttaca gctcttggag ttttaccaat gagttttgca aactcctgat aaggcacggc
     4561 atcagtcacg cttacaagct gtctattcat ctgctacgat tctcccttag tgcttctaat
     4621 ggctcctaat ggctaattat tgcctaaaag gataactcca gaagcacaac atttcacact
     4681 atcagcaaga aattacgcaa tcggagtaat tatgtcaata gacgtttcgg agaagttgaa
     4741 gctaatccgt gaatctgaaa ggttaaaccg taaagaattc agtgaattaa ctggtgtagc
     4801 ctacagctca ctttcgagct atgagagccg gtcaaaaaac gctggagttg aagccataat
     4861 gaaggtctta caacatccta gatttactaa atatactttg tggttcatga ctgatcaggt
     4921 agctccagaa gccgggcaaa ttgcgcccgc tctcgcacac tttgggcaaa acgaaacaac
     4981 gtcgccccac tccggtcaaa agactggtta acaatttatc gtgaatatat tcatcacaag
     5041 tgcctactat tggtggctaa atttcagcca ccacgaaaaa agcgattagt agtagcaaaa
     5101 aaaagtacca ctcggagggt tttctgatgg caatcaaaaa actcgatgat ggtcgatatg
     5161 aagtggacat ccgccctact ggacgtaacg gaaaacgcat ccgtaggaag tttgataaga
     5221 aaagcgaagc tgtcgctttc gaaaaataca cgttgtacaa ccaccacaat aaagaatggc
     5281 tatcaaaacc aacagacaaa cgacgtctgt cggaactgac acagatctgg tgggatttaa
     5341 agggtaaaca cgaagagcat gggaaatcta atcttggaaa aattgaaatc ttcacaaaaa
     5401 taacgaatga cccatgcgca tttcaaatca cgaaatccct tatcagccag tactgcgcca
     5461 cccgaagaag tcagggtatt aaaccttcga gtatcaatcg tgatttaaca tgtattagcg
     5521 gcatgtttac agccctgatt gaagcggagt tattctttgg tgagcaccct atcagaggga
     5581 caaagaggct taaggaggaa aaaccagaaa caggctatct cacacaggaa gaaattgcct
     5641 tactgcttgc tgctcttgac ggcgacaaca aaaagattgc gattctttgc ctgagcactg
     5701 gagcacgttg gggagaagca gctcgtttga aagcagaaaa tatcatccat aaccgcgtca
     5761 cgtttgttaa aacgaaaaca aacaaaccac gcaccgtccc gatctcagag gctgttgcca
     5821 aaatgatcgc ggataacaaa cgaggttttt tattccctga tgctgattac cctcgcttca
     5881 gacgaacaat gaaagcaata aaaccggatt tgccaatggg gcaagccaca catgcactaa
     5941 ggcacagctt tgccactcat ttcatgatta atggaggaag tattatcacg ctacaacgga
     6001 tactaggtca cacgcggatt gagcaaacta tggtttacgc tcattttgcg ccagagtacc
     6061 ttcaggacgc catttctctt aatccgctaa gaggtggtac tgaggccgag agtgtccaca
     6121 cagtgtccac agtagagtaa cgtttaaggg ctttcagtgg taatttatgc cgctcaaacc
     6181 cgcattgtac cgttgaaagc ccctactggt gacaccctaa atctccctta cacgggctta
     6241 ttttttatac ataaaccata gagacggatt gatcccaggt caccgtcttc cattgaccac
     6301 atcgatagaa tctcccttca tagcacgatg cctttcactt attggcatcg tgctcgcaca
     6361 ggttccggtt acgcacagcc agaacgcgca tgtttgatgc ttaccaaaaa atattctcac
     6421 tctccacatt tgaatgtcag acgagcgacg ccatgtaatc ctgcaccttc tgtcttcagg
     6481 tcaactatct gcattttttt gccctgagta acacagaaat gaactgcatc attttttact
     6541 atattttctg caccagatat tctacccctg gctaaagaag cttcggcttc ggtgtagtat
     6601 tggttatcga gtttacgctg aatattactt ttatatgcaa gaccaaattt accgatactt
     6661 gtctcatcat tatgtacagc acacccagac ataagaaaaa cactaattaa tgatatagca
     6721 gctatctttt tcatctcacc ttcccccatt aaataccaac gacacgctct ggtgtttaaa
     6781 tataataatg gcatgattat tataattgaa taggattata ataaatgttc tgtacaacat
     6841 ttcctacata agtaggaatt acatacacgg atgcccttcc aggtaacccc atgggggggt
     6901 taatat
//
LOCUS       DAZKGF010000217         6904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZKGF010000217 DAZKGF010000000
VERSION     DAZKGF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.217"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(467..1357)
                     /gene="ybcH"
                     /locus_tag="ACHOQV_004533"
     CDS             complement(467..1357)
                     /gene="ybcH"
                     /locus_tag="ACHOQV_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFP9632803.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFIH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
     gene            complement(1358..4330)
                     /gene="nfrA"
                     /locus_tag="ACHOQV_004534"
     CDS             complement(1358..4330)
                     /gene="nfrA"
                     /locus_tag="ACHOQV_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFP9632804.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSGNPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYTVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHGMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRH
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPRKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGIEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(4317..6554)
                     /gene="nrfB"
                     /locus_tag="ACHOQV_004535"
     CDS             complement(4317..6554)
                     /gene="nrfB"
                     /locus_tag="ACHOQV_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFP9632805.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNPEQVAQLES
                     ENEGE"
     gene            complement(6752..>6904)
                     /locus_tag="ACHOQV_004536"
     CDS             complement(6752..>6904)
                     /locus_tag="ACHOQV_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP9632806.1"
                     /translation="AAELFSGLRHIAINILTNDKVFKAGLRRKLRKAAMDRNYLASVL
                     AGSGLS"
BASE COUNT         1629 a         1794 c         1796 g         1685 t
ORIGIN      
        1 tctttgtgga gtttttatct attcaagtac catgccttta gatgcatatt agcgataata
       61 gcatgaggtt tatcctcaat tgctgtgttt tttagtataa aaaagaggaa caaaactgag
      121 acacataagg cctcgcaatg gcttgcaagg ttttacatat tttgaggtgg tggaacgtgt
      181 gaacgcagag atggcgcggt aatttgttga tttaaaatgt cgttcctgga gtttctaagt
      241 cgtgggctac aggttcgaat cctgcagggc gagccatttc ctcgccattt atttgttccc
      301 ttttacaaat tacaccatca ctttgttgtc gcggttttgc taaataattg gaacacgtca
      361 gacgaataaa atgaaaacag aaattctgct aaacaattca tcttgccagg attttgatta
      421 ggaaagtgaa aaggaaagga gcaagctaag ttgctccctt taatcattaa tactcaagga
      481 tactctgcgc gacgggcaaa tagcgcagag agaaatagag cgtgtcttta ccgcaagagg
      541 aacgtttcgc taacagcgag gcgatctctg catccggttt cggtttcgct gtaaacgttt
      601 tacctttacc agcgacaaac agttcataaa caatgccgct tgctggggcg gaactttggc
      661 aatcggcgct ggcctgtaga taacgttcac gctgttcagc ggtcagttta tccacgccgc
      721 tgccatcctg tacccagaca ttaacaccgg ttgctttaac ttgttccagc agttggcgat
      781 agccatctgg cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa
      841 catcgctaat caaccgctgc gcgttgttta accatgttag caaaggttga cgggcggctt
      901 cgctgcgcca gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttacgc
      961 caggcacggc gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat
     1021 agctttccag cgctgcggag gactgtttct ggtgtataaa aaattccgga tcggcgttca
     1081 gcccgacaat cagcttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg
     1141 cgcgctgttc tggctgggtg aatgcatcgc cgtaacgggt ccattgcaaa accagggtat
     1201 cgaagccttg caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac
     1261 tatcacggtt ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca
     1321 aaagcagtgt caacaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga
     1381 gaaacgcgtt gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatatt
     1441 cgatgccgag actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat
     1501 tccagcggac cccgacaccg ccaagctgcg cgccctgagt gcctttatca cgataaccgt
     1561 tgtcctgaac gtgagcataa ggctcaatag tctgcccgtt tgccaccttc tgatgccagc
     1621 tgactcgata atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga
     1681 ggtacaggtt ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc
     1741 cgccattaaa gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgtccgt
     1801 tcagcggcaa ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc
     1861 gcagaccggt gccggacatc ggatttttca ccggcatcat caccccgttt tcaccggtat
     1921 cggcaaagac gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga
     1981 tgcggtactc ggcttccagt tgcccgtagc tacgatagct tttccttggc gctgcgccgc
     2041 cgacattatt gttagcggta ctcattgcgc cggaacgcaa gccaatagaa gaatcgaaac
     2101 tgaacgtcca gtggcgaccg acctcctcat gcagacggcg gaaattgaag cgttgctgat
     2161 tttgctctgg ggtcagtggg gttatcagcg cctgattatc aatgtcatca atcaccagtc
     2221 gggcgtagtg ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc
     2281 ggataagtgc cggatcgtcc ggtagccctt tatgcgcctg ttcgagcatt tcccgcgact
     2341 gtgcgatatc accgctatcc cacaaggcgt aaccgagcgc tgcctgggtg ttgctattat
     2401 tcggttccag ttccagagcg gcgcgcaaat cactcaccgc cgccgggaca ttatgacgtt
     2461 ggcgataaat tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc
     2521 gcgtgagatc gctcagtgcg agttccggct gaccaggaat gtaacgttgc gcatgcagcc
     2581 accagtagag ggcattgttt cccagcccac gttgttctgc ctgctgaagc cagcgatcgc
     2641 gagccgcacc atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc
     2701 tcatgccgtg aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct
     2761 gatacgcctg ataggctacc gcacgatgtt gccaggcgtt cggttgtcgt tgttcggcct
     2821 gaagccatgc atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt
     2881 tccaggcggc agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc
     2941 aattatctgc aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg
     3001 gtttcgataa aatcgccacc ttcgccggag ttgccaggta aggatgactt tccagcagag
     3061 acgccagtcg cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt
     3121 gcagcaataa atcggcagct tcacgtgact gctcgttctg catcagttgc caggtcagtt
     3181 gatccaggcg ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag
     3241 cttcagcctt gttacgggtc gccacgctaa cggtataacg ctcctcaagc atttcattgg
     3301 cggggagggt ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg
     3361 gtagcgtcgc gccaacaaca tactggcggt tgtcggcaaa ctgtaccgta taattcgcca
     3421 acgcctgaac ggggttgccg ctgtatttag ataacagata gagccaactt ttctcttgtg
     3481 cgtccgtggt aaatagcggc gtattttcaa tgagataatg ctggaggcgt gctttttcgc
     3541 cacgataagc cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct
     3601 gtgattgcag cgccaggatc cgatcgtcca gctgcccggc aagaagcaca tcaaaccact
     3661 gacggcgttc tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg
     3721 cctgggacca ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc
     3781 cttccggcga tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaattgtg
     3841 ccagccgcag ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc
     3901 acgctttttg ctgggcaagc agttcttcaa cagtcgtaac gcttttcact tcaactggaa
     3961 tagccgccag actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa
     4021 gcaacagccg cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag
     4081 tcagcggaat attatccggc acctgttggt gtatatattc aaattcgcgg atggcggttg
     4141 cttcgtcgtt atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat
     4201 ggcgataatc gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc
     4261 tggtgctcaa taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat
     4321 tctccttcat tttcggactc cagttgcgca acctgttctg ggtttaaacc tgctttgagt
     4381 aatagtgatt gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt
     4441 tcctggctga taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca
     4501 tggcgcaata acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt
     4561 tcggcgaaca ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc
     4621 gtgagccact gatgctgaac cgcattgtac aacattgccc gcgggtcgtg accgcgtcgg
     4681 cgtgcatacc agtgacgtaa ccccgtgaca atttgtccgc gcagaacaat gacgtaacgc
     4741 actttgcgtc cgactttacg cgtcagggct gccagcgaaa ccgggtcaat accatcttca
     4801 ctgccgacaa ttaactcatc attttccaga cgcagcggca gcactgcata atgcagcgcc
     4861 acggaagccg gcatttcggc aatcagtgat gaagggatct gccaggcgtc gatcgactcc
     4921 cacgccacgc cgttttgctc tgccagcgcc tgtgccagct gttcggcgct aatcagcccc
     4981 tgcatcagca ttgaaccgcc caggcgtaga ccttcgacgc gattacgcag tgctgtatcg
     5041 agttgttctt cagtgatgac ctgattttcc agcaggattt ggcctaacgg gcgcaacgag
     5101 cgggtatcgc cagtcacgct ggggaagtca tgcgttgttt tatcccacgc gacgcgacgt
     5161 ggatcgccgt gttgaagtac ctgttttaac gcgcgccagt tggccatgaa gttaatcagg
     5221 ttgccccaga aaagacgcag gacagatagc agcccctgcg tcagaccgta gtagccagtg
     5281 acaaaaatca cccgctgcac gatgcggtta accatcaaac caaagtttag ccacagcagg
     5341 gtcattaacc atgcgctgcc gctgaaaata gaaaggaaat gccaggcatc gggccacaaa
     5401 ctttcatacg ccagtaacag caaaagctgg atcatcacca gcatcgcgag gaagctgaca
     5461 aagttactga ttgccccttt gcggtcgcgc cagagaaagt agttcagcgt caggctggag
     5521 gtccatttgt gggttttaaa gccctggaaa acaatgccga tgatccagcg agatttttgt
     5581 cgaaccgcag tcgaaaaggt atcggggaaa tattcgcgca cgcagatcat gtttgacgtc
     5641 cgcgcgtgct gtaaaaattt acgctgctcg cgttctttgg cttcgtccac caccggaaaa
     5701 cggacaaaaa tttccgtcat acctttttct ttcaggcgga aaccaatgtc gtaatcttca
     5761 gtcagactct gcacgtcgaa agcaataccg tcaccgtcag ctaacagtgc ggtcacggcg
     5821 cggcggctga aacaggtgcc gacgcctgcg ctgggcacct gtccggcgag ggcttcacgc
     5881 accggaacat ctttgccatg cagttctgaa aactcatcaa tgtaggtcat gctggtgaag
     5941 tgcgtccatt cgcgttcgaa cggatacact gggatctgaa tcagatcttt acgctcgacc
     6001 agatagttga acagacgcaa ttccatcggt gaaatcacat cttcggcgtc atgcagaata
     6061 aaaccagcaa aagcgaaatt ggcgctacgt tcaaattggg tgatggcgtc cagcacgttg
     6121 ttcagacagt cggctttgct ggtggggcca ggacgcgcgc agactacctt atgcacgttc
     6181 gggaagcgag cgcacacttc gtcaacatca cgctgagtat cggggtcgtt ggggtaggtg
     6241 ccaacaaaga tatgatagtt ttcgtagtcg agcgtggtcg ccgccagctc ggccatattg
     6301 ccgatgacgc ccgtttcatt ccacgccgga accataatcg ctaacggttt ttcatctggt
     6361 ttatacagtt cgcggtaact cattcgcggg tagcggcgat aaacactcaa cttgcgttta
     6421 atgcggcgta cccagtagac gacatcaata aaaaaatcgt ccagcccgct gatgaacatg
     6481 atgaccgcta acgttatcgc gattactttt aagccgtaga gccaggtagc aaaaacatca
     6541 agaagccagt ccacacaaaa accttacatt aacgctggtt atgtttaggg tggcgtatat
     6601 taaggttttt tatgaattgt gacagctttt taccattaat aggtatgact attgcggcac
     6661 gttattttta cactggttat aaaagttgcc gtttgcgtag aatgtagaca aaagcgtggt
     6721 gtcaaggagg ggaggttaac gcagggaaag attacgaaag cccgctccca gcaaggactg
     6781 acgcgagata gtttctgtcc atggctgctt ttcgcaactt acgtcttaac cctgccttga
     6841 ataccttatc attcgtcaaa atattaatag cgatgtgccg tagccctgaa aataattctg
     6901 ctgc
//
LOCUS       DAZKGF010000218         6903 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZKGF010000218 DAZKGF010000000
VERSION     DAZKGF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.218"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            416..1405
                     /gene="araF"
                     /locus_tag="ACHOQV_004537"
     CDS             416..1405
                     /gene="araF"
                     /locus_tag="ACHOQV_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFP9632807.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1475..2989
                     /gene="araG"
                     /locus_tag="ACHOQV_004538"
     CDS             1475..2989
                     /gene="araG"
                     /locus_tag="ACHOQV_004538"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFP9632808.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRTHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     ADQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3004..3990
                     /gene="araH"
                     /locus_tag="ACHOQV_004539"
     CDS             3004..3990
                     /gene="araH"
                     /locus_tag="ACHOQV_004539"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFP9632809.1"
                     /translation="MSSVSTSGSGAPKSSFSLGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4157..4957
                     /gene="otsB"
                     /locus_tag="ACHOQV_004540"
     CDS             4157..4957
                     /gene="otsB"
                     /locus_tag="ACHOQV_004540"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFP9632810.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTAIAQFPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4932..6356
                     /gene="otsA"
                     /locus_tag="ACHOQV_004541"
     CDS             4932..6356
                     /gene="otsA"
                     /locus_tag="ACHOQV_004541"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFP9632811.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6363..6791)
                     /gene="uspC"
                     /locus_tag="ACHOQV_004542"
     CDS             complement(6363..6791)
                     /gene="uspC"
                     /locus_tag="ACHOQV_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFP9632812.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1726 a         1637 c         1807 g         1733 t
ORIGIN      
        1 gcgaaatgaa tgacgcacat tttccacatc tttgagttgt ggttattaca ccattgcaaa
       61 aactcaacgc caggtaatgc ggcctattga ctggttaaaa agaagacatc ccgcatgggt
      121 accaaagaca acaaggactt ccaggctaat cttatggatt attctgctgt gcactcgata
      181 atttgtctga caaattcgct tttccttatg tcttttcccg ctaaatttat gcacgttctc
      241 actgtaattc tgcgatgtga tattgctctc ctatggagaa ttaatttctc gctaaaacta
      301 tgtcaacaca gtcacttatc ttttagttaa aaggtaatgc tttgttttcc gattaattta
      361 acgaatgtca ttcgtttttg ccctacacaa aacgacacta aagctggaga gaaccatgca
      421 caaatttact aaagccctgg cagccattgg tctggcagcc gttatgtcac aatccgctat
      481 ggcggagaac ctgaagctcg gtttcctggt gaagcaaccg gaagagccgt ggttccagac
      541 cgaatggaag tttgccgata aagccgggaa agatttaggg tttgaagtta ttaagattgc
      601 cgtgccggat ggcgaaaaaa cattgaacgc tatcgacagc ctggctgcca gtggcgcgaa
      661 aggtttcgtt atttgtactc cggacccgaa actcggctct gccatcgtgg cgaaagcgcg
      721 tggctacgat atgaaagtca ttgccgtgga tgaccagttt gttaacgcca aaggtaagcc
      781 aatggatacc gttccgctgg tgatgatggc tgcgaccaaa attggcgaac gtcagggcca
      841 ggaactgtat aaagagatgc agaaacgtgg ctgggatgtc aaagaaagcg cggtgatggc
      901 gattaccgcc aacgaactgg ataccgcccg ccgccgtaca acgggttcta tggacgcact
      961 gaaagctgca ggattcccgg aaaaacaaat ttatcaggta cctaccaaat ctaacgacat
     1021 cccgggggca tttgacgctg ccaactcaat gctggttcaa catccggaag ttaaacattg
     1081 gctgatcgtc ggtatgaacg acagcaccgt gctgggcggc gtacgcgcga cggaaggtca
     1141 gggctttaaa gcggccgata tcatcggtat cggtattaac ggtgtggatg cggtgagcga
     1201 actgtctaag gcacaggcaa ccggcttcta cggttccctg ctgccaagcc cggacgtaca
     1261 tggttataag tccagcgaaa tgctttacaa ctgggtagca aaagacgttg aaccgccaaa
     1321 atttaccgaa gttaccgacg tggtgctgat cacgcgtgac aactttaaag aagaactgga
     1381 gaaaaaaggt ttaggcggta agtaatttgc cggaaagatt cccctctgca tgatgcagag
     1441 ggggtgtgaa cgaccagtga ttcacggaga cgttatgcaa cagtctaccc cgtatctctc
     1501 atttcgcggc atcggtaaaa cgtttcccgg cgttaaggcg ctgacggata ttagttttga
     1561 ctgctatgcc ggtcaggttc atgcgttgat gggtgaaaat ggcgcaggaa aatcaactct
     1621 cttaaaaatc ctcagcggaa actatgcgcc aaccacgggt tctgtagtga ttaatgggca
     1681 ggaaatgtcc ttttccgaca cgaccgcagc acttaacgcg ggggtggcga ttatttacca
     1741 ggagctgcat ctcgtgccgg aaatgaccgt cgcggaaaac atctatctcg gccagctgcc
     1801 gcataaaggc ggcattgtca atcgctcatt gctgaattat gaggcgggtt tacaacttaa
     1861 acatcttggt atggatattg acccggacac gccgctgaaa tatctctcca ttggtcagtg
     1921 gcagatggtt gaaattgcca aagccctggc gcgtaatgcc aaaattatcg cctttgatga
     1981 gccaaccagc tccctctctg cccgtgaaat cgacaatctt ttccgcgtta ttcgtgaact
     2041 gcgaaaagag gggcgggtga tcttatacgt ttctcaccgt atggaagaaa tatttgccct
     2101 cagcgatgcc atcactgtct ttaaagatgg acgttatgtc aaaaccttta ccgatatgca
     2161 gcaggttgac cacgacgccc tggtgcaggc gatggtcggg cgcgacatcg gcgatatcta
     2221 cggttggcaa ccgcgtagtt atggcgagga gcggctgcgt cttgatgctg tgaaagcacc
     2281 aggcgtgcgt acgccaataa gtctggcggt tcgcagtgga gaaattgtcg gtctgtttgg
     2341 tctggtcggg gcagggcgta gcgaattaat gaaaggcatg tttggcggta cgcaaatcac
     2401 cgccggtcag gtttatatcg accaacagcc gatcgatatt cgtaaaccga gccacgccat
     2461 tgccgcaggc atgatgctct gcccggaaga tcgcaaagcg gaaggcatta ttcccgtgca
     2521 ctccgttcgc gacaatatca acatcagtgc cagacgtaca catgtgctcg gcggttgtgt
     2581 aatcaacaac ggttgggaag aaaacaatgc cgatcaccac attcgttcgc tcaacatcaa
     2641 aacgccgggc gctgatcaac tgatcatgaa tctctcaggc ggaaatcagc aaaaagccat
     2701 tctgggccgc tggttatcgg aagagatgaa ggtcattttg ctggatgaac ctacgcgcgg
     2761 cattgatgtt ggtgctaagc acgaaatata taacgtaatt tatgcgctgg cggcgcaggg
     2821 tgtggcggtg ctgtttgcct ccagcgactt acctgaagtc ctcggcgttg ccgaccggat
     2881 tgtggtgatg cgggaaggcg aaatcgccgg tgaattgtta cacgagcagg cagatgagcg
     2941 tcaggcactg agccttgcga tgcctaaagt cagccaggca gttgcctgag taaggagagt
     3001 atgatgtctt ctgtttctac atcgggttct ggcgcaccta agtcgtcatt cagcttaggg
     3061 cgtatctggg atcagtacgg catgctggtg gtgtttgcgg tcctttttat cgcctgtgcc
     3121 atttttgtcc cgaactttgc cacctttatt aatatgaaag ggttgggtct ggcaatttcc
     3181 atgtcgggga tggtggcttg tggcatgttg ttctgccttg cttccggtga ctttgacctt
     3241 tctgtcgcct ccgtaattgc ctgtgcgggt gtcaccacgg cggtggttat taacctgact
     3301 gaaagcctgt ggattggcgt ggcagcgggt ttgttgctgg gcgttctctg tggcctggtc
     3361 aatggctttg ttattgccaa actgaaaata aatgccctga tcacgacact ggcaacgatg
     3421 cagattgttc gaggtctggc gtacatcatt tcagacggta aagcggtcgg tatcgaagat
     3481 gaaagcttct ttgcccttgg ttacgccaac tggttcggtc tgcctgcgcc aatctggctc
     3541 accgtcgcgt gtctgatcat ctttggcttg ctgctgaata aaaccacctt tgggcgtaat
     3601 accctggcga ttggcgggaa cgaagaggcc gcgcgtctgg cgggtgtacc ggttgttcgc
     3661 accaaaatta ttatctttgt tctctctggc ctggtatcag cgatagccgg aattattctg
     3721 gcttcacgta tgaccagtgg gcagccaatg acgtcgattg gctatgagct gattgtgatc
     3781 tccgcctgcg ttttaggtgg cgtttctctg aaaggcggca tcggaaaaat ctcatatgtg
     3841 gtggcgggta tcttaatttt aggcaccgtg gaaaacgcca tgaacctgct taatatttct
     3901 cctttcgcac agtacgtagt tcgcggctta atcctgctgg cagcggtgat tttcgaccgt
     3961 tacaagcaaa aagcgaaacg cactgtctga tgcttttttc cacaacaatt tagcgttttt
     4021 tcccaccaca gccagccgcc acaacggttg gctgttcttc gttgcaaatg gcgacccccg
     4081 tcacactgtc tatacttaca tgtctgtaaa gcgcgttctg cgcaacacaa taagaaaaga
     4141 gaaggaggag aaccgggtga cagaaccgtt aaccgaaacc cctgaactat ccgcgaaata
     4201 tgcctggttt tttgatctcg atggaacgct ggcggaaatc aaaccgcatc ccgatcaggt
     4261 cgtcgtgcct gacactattc tgcaaggact tcagctactg gcaaccgcaa gtgatggtgc
     4321 attggcattg atttcagggc gctcaatggt ggagcttgac gcactggcaa aaccttatcg
     4381 cttcccgtta gcgggcgtgc atggggcgga gcgccgtgac atcaatggta aaacgcatat
     4441 cgttcatctg ccggatgcga ttgcgcgtga tattagcgtg caactgcata cggctatcgc
     4501 ccagtttccc ggtgcggagc tggagacgaa agggatggcc tttgcgctgc attatcgtca
     4561 ggctccgcag catgaagacg cattaatgac attagcgcaa cgtattactc agatctggcc
     4621 acaaatggcg ttacagcagg gaaagtgtgt tgtcgagatc aaaccgcgag gcaccagtaa
     4681 aggtgaggcg attgctgcct ttatgcagga agctcccttt atcgggcgaa cgcctgtatt
     4741 tctgggcgat gatttaaccg atgaatctgg cttcgcagtc gttaaccggc tgggcggaat
     4801 gtcagtaaaa attggcacag gcgcaactca ggcatcatgg cgactggcgg gcgtgccgga
     4861 tgtctggagc tggcttgaaa tgataaccac cgcattacaa caaaaaagag aaaataacag
     4921 gagtgatgac tatgagtcgt ttagtcgtag tatctaaccg gattgcacca ccagacgagc
     4981 acgccgccag tgccggtggc cttgccgttg gcatactggg ggcactgaaa gccgcaggtg
     5041 gactgtggtt tggctggagc ggtgaaacag ggaatgagga tcagccgcta aaaaaggtga
     5101 aaaagggtaa cattacgtgg gcctctttta acctcagcga acaggacctt gacgaatact
     5161 acaacaaatt ctccaatgcc gttctctggc ccgcttttca ttatcggctc gatctggtgc
     5221 aatttcagcg tcctgcctgg gatggctatc tacgcgtaaa tgcgttgctg gcagataaat
     5281 tactgccgct gttgcaagac gatgacatta tctggatcca cgattatcac ctgttgccct
     5341 ttgcgcatga attacgcaaa cggggagtga ataatcgcat tggtttcttt ctgcatattc
     5401 ctttcccgac gccggaaatc ttcaacgcgc tgccgacata tgacaccttg cttgaacagc
     5461 tttgtgatta tgatttgctg ggtttccaga cggagaacga tcgtctggcg ttcctggatt
     5521 gcctttctaa cctgacccgc gtcacgacac gtagcgcaaa aagccatacg gcctgcggca
     5581 aagcgtttcg aacggaagtt tacccgatcg gcattgaacc gaaagaaata gcgaaacagg
     5641 ctgccggacc actgccgcca aaactggcgc aacttaaagc ggaactgaaa aacgtacaaa
     5701 atatcttttc tgtcgaacgg ctggattatt ccaaaggttt gccggagcgt tttctcgcct
     5761 atgaagcgtt gctggaaaaa tatccgcagc atcatggtaa aattcgttat acccagattg
     5821 caccaacgtc gcgtggtgat gtgcaagcct atcaggatat tcgtcatcag ctcgaaaatg
     5881 aagctggacg aattaatggt aaatacgggc aattaggctg gacgccgctt tattatttga
     5941 atcagcattt tgaccgtaaa ttactgatga aaatattccg ctactctgac gtgggcttag
     6001 tgacgccact gcgtgacggg atgaacctgg tggcaaaaga gtatgttgcc gctcaggacc
     6061 cagccaaccc gggcgtactt gttctttcgc aatttgcggg agcagcaaac gagttaacgt
     6121 cggcgttaat tgttaatccc tacgatcgtg acgaagttgc agctgcgctg gatcgtgcat
     6181 tgactatgtc gctggcggaa cgtatttccc gtcatgcaga aatgctggac gttatcgtga
     6241 aaaacgatat taaccactgg caggagtgct tcattagcga cctaaagcag atagttccgc
     6301 gaagcgcgga aagccagcag cgcgataaag ttgctacctt tcctaagctt gcgtaggagc
     6361 ggttaatctc ccgtaagtgg aactaacagc acatcgacct cacttgaggc aatcactctt
     6421 ttcgccgagc aggatgctcg cgaaaagaaa ctgtgattgt gattaccgca aatcaccaaa
     6481 tcgaaatgat atttgcgaca aacttccaga atatgttcgc ttaactcacc gtaggcaata
     6541 aacgtcttgt caacgggata ccccgcatcc tgaattaact tatcaagaaa gctttgcgtt
     6601 tcttcctgca tcacgctgcg taaatcttca agcatcggcg cagctaattg attgtacatt
     6661 tccgggtcgg aagcgagagt aattaaactg atgtgtccct ttactggcct ggcgatagat
     6721 accgcttttg ccagcagttg ctgactttcc ggtgtaaccg caacagcgac aagaatattg
     6781 ctatagctca taacctgttc cttattctgt gaacttcagg tgacattaaa gctttactcc
     6841 taatggagaa acgacgcaat cccaactcag tcaaacaggt aaatattagc gtgattatta
     6901 gta
//
LOCUS       DAZKGF010000219         6863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZKGF010000219 DAZKGF010000000
VERSION     DAZKGF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.219"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(381..1206)
                     /locus_tag="ACHOQV_004543"
                     /pseudo
     CDS             complement(381..1206)
                     /locus_tag="ACHOQV_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1357..2130)
                     /locus_tag="ACHOQV_004544"
     CDS             complement(1357..2130)
                     /locus_tag="ACHOQV_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9632813.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTLTERGRD
                     ALSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIARGFRAERIH
                     ATGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGQSVR"
     gene            complement(2155..6483)
                     /locus_tag="ACHOQV_004545"
     CDS             complement(2155..6483)
                     /locus_tag="ACHOQV_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP9632814.1"
                     /translation="MNLRKKHICIAISLVLNPFPASLAAQNTNVVEVDETDKGYQVYG
                     DNWKSIEIGTTKEVKKKNSTDLNFDHNSPVAKGHNSIAIGTSAEAGGEIKDEKGFKTG
                     ESIAIGAKAKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGDRFGDKEIPNNYSSS
                     EASGDGSIVVGSRSTAAGDLSVSLGSISSANGTAANALGATAEANGDMATAVGFNALA
                     NASNALAVGSQSSAGGEDSLAIGSQSTTGKKSTVALGQGATASAAEGNVAIGADSVDK
                     AATPVSGATIKLKNGGTIEYRGFAGDKAASVVSVGDAGKERQIVNVGAGAVSEKSTDA
                     INGSQLYAIAKTLKDDLDAINPTWKLKDSTGKEKEVGSKNGGTTGNEVTVTGKGDIDV
                     TVDGNGLSIDGTKLAENIKKDAIKIKTGDGKTLTKKLGEEVEFNDAGPIKTKINNNKL
                     EITAETASFTPDGNGGNGKITVSNGDDDKLVTAKNIADAINQSGWKIKAGSENGGKVE
                     GDNKEELINPGDTVTLKAGKNLSVKQSNGDFTFSVVDTPSFSSVQLNGKGNNGNVTLS
                     SEGNKLTLSGGNGAGQNGVVISGVASGLEGKELSTLTNTDKEMKNAASVADLKTAIDG
                     VSTSMQGAGFALKDSDDKEVKQSLGQAIKVSGDSNITTTAITNGDKGLKISLNNDLSV
                     GSNKAPGSIKVNGTDGKEAVSLNGQDGTIKLTSAPAANGKSASATIAVNAGKGGLTDA
                     VGTTKTRLTYTPDGKSPAEELATMNDGLSFEGDDGKIIHKKLNEKLTIKGGAGDNEDV
                     TDSNIRVDANEKGDGLLVRMTRNLRDLASAVFGTENGPTTRIDNAGITITTPAAGGGQ
                     GNTVSLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAANIGDLKNALRDSSAAL
                     VNTGFGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVRLADNIRLADDGSVS
                     VGGTRMDKNGLTIKDGPSVTVNGIDAAGRVISGVKDGVNDQDAVNVSQLNREISQATA
                     ATTWALKTESGDEAVAVSSQTVEVRHGQNTRVSAISKDDKGNYSYEIDVTGIPVEYTD
                     SQGNPLVNIGGRFYTQTEDAATGKLTMTPAEPARVRISSEQPLVLTNVAAGNVSPVST
                     DAVNGSQLASAARIAGGDNVVWKDGKAALAPDTFSELTTAGGGKTTVNGGVHKTPDNV
                     ADAVNMLNREGTKYFKAASSGQAAKAEGRDSIAVGQGAVSRGQNSIAMGNGAEVTRQA
                     AAGSVAVGSHARAGRANTGTYALNGQAVAGRTGSDTAVVSFGQPGQERQLQSVAPGVL
                     SANSTDAVNGSQLHATNRQVAGNTQAINTLGNKFSQLSYRVEELNRDIRGVGASAAAM
                     SGIPQAYLPGKSLMGLGVGGYGGESAIAIGVSRISDNGKMIMKLNAGQNTRGNFSVGA
                     GVGWQW"
BASE COUNT         1632 a         1926 c         1373 g         1932 t
ORIGIN      
        1 ataaaccaac aagatagaaa atcatcacaa caaagggaaa ggtctgtaga taaagagtgg
       61 gatggacttc ttacttcata tcacataatt gatatttttt atcctgcaaa ctccaacggc
      121 attatgtatg acttagcctc aggcaattat cacaggcaaa aaaacaatat attgtaagct
      181 tcatagaatt tggaacttta tataataaat aatgctgagt tttttatcac caatacgtta
      241 acaaaattaa tgtattccag aggtttcaca ttagtctcca atttttcaga agaatctatt
      301 tacctggcag gaaaagtcat ctggtgtttc gccggagtga ggggtacagg caatagcgga
      361 cataaccatt tgttcttttt ctaagcagcc atctgataat attttatcct gaaggctgtc
      421 ggggagatat tccccagacg agagtgacga cgctgacgat tgtagaaaat ctcaatgtat
      481 tcccgtatta ctgagatggc ctcatcccgg cttttaaaac gatagtggct cagactttca
      541 tttttcagcg ttccccagaa gctttccata ggagcattgt cgtaacagtt aactttacgc
      601 gacattgata ttttcagacc aaactgctcc tgtatggccc tgtaatcgta tgcgcagtac
      661 tgtgaaccac gatcggagtg gtggattagc ccggtaggtg agcgctggtt cctgagcgcc
      721 ataaacaggg ctttacccgt caactatttc gtcatgcgct ctcccatggc gtaaccgaca
      781 attttgctcg tataaacatc tttgacgcca gcgatataca gccatccttc cggggggcaa
      841 catacgttat atctgcaacc aagacctgat taggtgctgt aggcgtaaag ttctgattca
      901 ggagattcgg cgcaaccggc agattgtgac aagagttcgt gttcgccctg aacttgcgtt
      961 tctgcttaca gtgcagcctc agctccttac gaagacgtgc cagtctgtca cgaccgacga
     1021 taattccgtt atctgccagc tccgtctgga gcagctaggt tccatatgtt tcgcgtgtgc
     1081 ggatatgtgc caccttaatc tccagtttta gccgctcatc actttgtttt ctgtctgagg
     1141 gtcatgctgt actcagtcgt aataaccgat cctgtataca ccaagtaccc gacacatcgc
     1201 ttcaatacat aatcgcatct gaatcgttca ccgattcgcg gcatttttca aggtagttgt
     1261 cgccattgtt cgactaactg atcgacatta cagcctagag cctagctggg ggctgtgcac
     1321 aaaaaagccc cggacattcc ggggctacac tgcatattaa cgaacgctct gtccggttat
     1381 ttcaaccctg cggttggggc gcagacattc cttcagtgct tcgccctcca catccggaca
     1441 gaacaccact ggtgcatcat atccacggcc cgttgcatgt atcctttcag ccctgaatcc
     1501 ccgggcaata aaccaccccc tgactgattc tgcccgtttc tctgacagga gccggttata
     1561 cgcctgcccg ccaagatggt ccgtaaatcc ttctatcttc accccgacaa catgacccgc
     1621 ctgaaacttc tctgccacac gggaaagcgc atcacgcccc ctctctgtca gagtccatga
     1681 atcaaaatca aataatatat caccggggac tgactccctg tattctgctg tctctgcctc
     1741 cggcagacag ttttccggct tccagtagaa agagcgggca tccatgttcc ggtcaaacag
     1801 tatcttgtac tggcaggtat aatccccctc cggtccggta aagttgaaca ggtaatccca
     1861 ttcccttaca ccagacatcc cttcagcaaa gtgtggtctc cccgccagct gataaatctg
     1921 gtccttattc atacccgcct ttatctgacg taacttttct ggcagtggcc acgaaccttt
     1981 ctgtgttccg tcatgattaa acatcacctt ctccggagaa ggccatacca cttcttccgt
     2041 cgttccttca tccgtgacat gactcagatt tccacatcct gcaagcatca gggacagcac
     2101 acccgtcatc atcattcgac taaataacat tacatttctc cttgcttttc cctgttacca
     2161 ctgccagccc acaccggcac cgacggagaa attaccccgg gtgttctgac ctgcattcag
     2221 tttcattatc atcttaccgt tatcggagat tcgggacaca cctattgcaa tggcgctttc
     2281 accaccatat ccaccgacac ccagccccat cagactttta cccggaagat aggcctgagg
     2341 tatcccgctc atggccgcag ccgatgcgcc aacaccacgg atatcccggt taagctcttc
     2401 cacccggtag ctcagctgac tgaatttatt cccgagagtg tttatcgcct gtgtatttcc
     2461 ggcaacctga cggttagttg catgcaactg actgccattg acggcatccg ttgaattcgc
     2521 agacaacaca cccggtgcca ctgactgcag ctgacgctcc tgtcccggct gaccaaaaga
     2581 aaccaccgca gtatcacttc cggtacgtcc cgcaacagcc tgaccgttca gggcataagt
     2641 cccggtattc gcgcggccag cacgggcatg gctgccaact gcaacagagc cggcagcagc
     2701 ctgacgcgtg acttcagcac cattacccat ggcaatgctg ttttgtcccc gggaaacggc
     2761 cccctgtccg acagcaatac tgtcacgccc ttccgctttt gctgcctgac cggaagaggc
     2821 agctttaaaa tacttcgtgc cttcacggtt cagcatattc actgcatcag ccacattatc
     2881 cggtgtttta tgtacaccac cattcaccgt tgtcttaccg ccacctgcag ttgtcagctc
     2941 actgaaggtg tcaggagcca gcgctgcttt tccgtctttc cagacaacat tatcaccgcc
     3001 agcaattctg gctgcagatg ccagctggct cccgttaacc gcatccgtgg ataccggtga
     3061 cacatttccg gccgccacat ttgtcagaac aagaggctgc tcactgctta ttctgacacg
     3121 ggccggttca gccggcgtca ttgtcagttt cccggtcgca gcgtcttcgg tctgtgtgta
     3181 aaaacgacca ccaatattga caagcgggtt cccctggctg tcggtgtact ccaccggaat
     3241 ccccgtcaca tcaatctcat agctgtaatt tcccttgtca tccttactga tggctgaaac
     3301 ccgggtattc tgtccgtggc ggacttcaac tgtctgggaa gaaacagcaa cagcctcatc
     3361 accggattca gtcttcagtg cccaggtcgt tgctgccgtt gcctgactga tttcacggtt
     3421 cagttgtgaa acgttaacgg cgtcctggtc attcaccccg tctttcacgc cggaaataac
     3481 ccggccagcc gcatcaatac cgttcacagt cactgacggg ccatccttga tggtcagtcc
     3541 gtttttatcc atccgggttc cgccgacaga aacactgccg tcatctgcca ggcgaatgtt
     3601 atcagcgagt ctgaccgtca ggctcccgtt ctgaacagtc gtgctgatat tctgcccgtc
     3661 accaccaacc gtgatatgct tcccgagaga cgtgttcacc ttgcttccgt catccgcctg
     3721 cagaccaaag ccggtattca ccagtgccgc actgctgtca cggagagcat ttttcaggtc
     3781 accgatattg gccgcattat tcagaacatc tccaccggcg tcagcaagcg cagtgccgtt
     3841 cagaccactg gccacactgg ttatctgatt tccaccgtta ttcaggccgg agccggtaag
     3901 ggaaaccgta ttcccctgac caccgcccgc agcaggtgtt gtgattgtga tgcctgcatt
     3961 gtcaatccgg gttgtcgggc cgttttctgt accaaaaacc gcgctggcca aatcacggag
     4021 gtttctggtc atccggacga gaagaccatc tcccttttca tttgcatcaa cacgaatgtt
     4081 actgtctgtt acatcctcat tatcaccagc accacctttg atggtcagtt tttcattcag
     4141 ttttttatga ataatctttc cgtcatcacc ctcaaaagaa agaccgtcat tcatggtcgc
     4201 cagctcttct gccggtgatt ttccatccgg ggtataagtc agacgtgtct tcgtggtacc
     4261 gacagcatct gttaatccac ctttgcctgc attaacagca atggttgctg acgcagactt
     4321 tccgttcgca gcaggcgcgg atgtcagttt aatcgtgcca tcctgaccat tcagactcac
     4381 agcctcctta ccatcagtcc cgttaacttt gatgctgccg ggagccttat tgctccccac
     4441 gctcaggtcg ttattcaggg atatttttaa ccccttatcc ccattggtga ttgctgtggt
     4501 ggtgatattg ctgtccccgg atactttaat cgcctgccca agagactgtt tcacttcctt
     4561 atcatcagag tctttcagtg cgaaacctgc accctgcata ctggtggaca caccatcaat
     4621 tgccgttttc aggtctgcaa cactggcggc atttttcatc tctttatcag tattcgttaa
     4681 cgtcgaaagt tccttacctt ccagtccact tgccacacca ctgataacaa caccattttg
     4741 tccggcacca ttaccgcctg acaatgtcag cttattacct tcggatgaga gcgttacatt
     4801 tccgttattt ccttttccat tcagctgaac actgctgaat gacggagtat caacaacaga
     4861 gaatgtaaaa tcaccgttac tttgtttcac gctgagattt ttaccggctt tcagtgtaac
     4921 tgtatcaccg ggattaatta gctcctcttt attatccccc tcaactttcc caccattttc
     4981 actgccagct ttaattttcc agccagactg gtttatggca tcagcaatat ttttcgcagt
     5041 aacaagctta tcgtcatcac cattgcttac tgtaatcttc ccgttaccac catttccatc
     5101 aggcgtaaaa ctggctgtct ctgcagttat ctcaagtttg ttattattaa tttttgtttt
     5161 tattggtccg gcatcattaa actcaacttc ttcaccaagt tttttcgtta gtgttttacc
     5221 atctcccgtt ttaatcttga tggcatcttt tttgatgttt tcagcaagct ttgtgccatc
     5281 aatactcagc ccatttccat caacagtaac atcaatatcc cctttaccgg tgacggtcac
     5341 ttcattacct gtagtcccgc catttttact gccgacctct ttttcttttc ctgtgctgtc
     5401 ttttaacttc cacgtcggat taatggcatc aaggtcatct ttcagtgtct tcgctatcgc
     5461 atataactga ctaccgttaa tggcatctgt acttttctca gatacagcac ccgcccccac
     5521 gttaacaatc tgacgctctt tacccgcatc accaacagat actactgatg cagccttatc
     5581 tccggcaaag cccctgtatt caatagtccc gccattcttt aacttaatgg tagcgccgga
     5641 aaccggagtt gctgccttgt ccactgaatc agcaccgatg gccacgttcc cctccgcggc
     5701 agaggctgta gctccctgcc ccagagcaac cgtggacttt ttgcccgtcg tactctgact
     5761 ccctatagcc agggaatcct ctccaccggc agaggactga ctaccaacag ccagagcatt
     5821 actggcatta gccagcgcat taaaaccaac cgcagtcgcc atatccccgt tagcttccgc
     5881 cgtggctccc agtgcattag cagccgtccc gttggcagag cttattgagc ccagggacac
     5941 agacaggtct cctgcagctg tgctccttga gcccacaaca atacttccgt ctcctgatgc
     6001 ttcagatgac gagtaattat tcggaatttc tttatcaccg aatctgtcac catgcaaagg
     6061 ggttaaatca tctccaccaa taacaatagc cccccatccg gtagctttgg tatcgccacc
     6121 tattgcaatt gactgttctt tagttgcttt cgccttggca ccaatagcta tggattctcc
     6181 tgtcttaaaa cctttttcgt ctttaatttc acctcctgct tctgcactgg taccaatcgc
     6241 aatggagtta tgccctttcg ctaccgggga gttatggtca aaattaaggt cggttgaatt
     6301 ctttttcttt acttccttcg ttgtacctat ttcaatactt ttccagttgt caccataaac
     6361 ttggtaacct ttatctgttt catcaacttc aactacatta gtgttttgtg ctgccagtga
     6421 tgcagggaac ggattaagaa caagtgatat ggctatacag atatgttttt ttctgaggtt
     6481 catatatatc ctcttttctc ctttattttc ttccactcag tcggaatagc ataaaaatca
     6541 tgcatgaata ttatgtaagg tggactgcct tcatttcttc agcatccatt tatataaagc
     6601 cagaagtaaa aaaacaggaa ccattaaaaa gcagacaaaa ggcaataaag tgatttataa
     6661 taatgcaaac ataaaacata ttaagtaaaa tacccgacat tacgcgttac agcagaatgc
     6721 aggtacacta cctgccattg cctgacgcac tggcgaaggc agtaaaaatg agatacattt
     6781 cacttcgcag caaaaaaacg aaatcctgca attctttctg attttactga cagaaaagcc
     6841 tgagagttaa atttaaataa gtt
//
LOCUS       DAZKGF010000220         6859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZKGF010000220 DAZKGF010000000
VERSION     DAZKGF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.220"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            77..151
                     /gene="tnaC"
                     /locus_tag="ACHOQV_004546"
     CDS             77..151
                     /gene="tnaC"
                     /locus_tag="ACHOQV_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFP9632815.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            372..1787
                     /gene="tnaA"
                     /locus_tag="ACHOQV_004547"
     CDS             372..1787
                     /gene="tnaA"
                     /locus_tag="ACHOQV_004547"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFP9632816.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1879..3126
                     /gene="tnaB"
                     /locus_tag="ACHOQV_004548"
     CDS             1879..3126
                     /gene="tnaB"
                     /locus_tag="ACHOQV_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFP9632817.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3258..4433
                     /gene="mdtL"
                     /locus_tag="ACHOQV_004549"
     CDS             3258..4433
                     /gene="mdtL"
                     /locus_tag="ACHOQV_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFP9632818.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPTTSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFEHGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4408..5367
                     /gene="yidZ"
                     /locus_tag="ACHOQV_004550"
     CDS             4408..5367
                     /gene="yidZ"
                     /locus_tag="ACHOQV_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFP9632819.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5524..6273
                     /gene="yieE"
                     /locus_tag="ACHOQV_004551"
     CDS             5524..6273
                     /gene="yieE"
                     /locus_tag="ACHOQV_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFP9632820.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6295..>6859
                     /gene="yieF"
                     /locus_tag="ACHOQV_004552"
     CDS             6295..>6859
                     /gene="yieF"
                     /locus_tag="ACHOQV_004552"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9632821.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
BASE COUNT         1612 a         1670 c         1718 g         1859 t
ORIGIN      
        1 atttaaacaa tttcagaata gacaaatttc ttgagtatta tactgtagcc tcgtgtcttt
       61 cgaggataag tgcattatga atatcttaca tatatgtgtg acctcaaaat ggtttaatat
      121 tgacaacaag attgtcgatc accgcccttg atttgccctt ctgtagccat caccagagcc
      181 aaaccgatta gattcaatgt gatctatttg tttgctatat cttaattttg ccttttacaa
      241 aggccatctt tcgtttattt acttgtttta gtaaatgatg gtgcttgcat atatatctgg
      301 cgaattaatg gatattgcaa atgtaatatt cacagggatc actgtaatta aaataaatga
      361 aggattatgt aatggaaaac tttaaacatc tccctgaacc gttccgcatt cgtgttattg
      421 agccagtaaa acgtaccact cgcgcttatc gtgaagaggc aattattaaa tccggtatga
      481 acccgttcct gctggatagc gaagatgtgt ttatcgattt actgaccgac agcggcaccg
      541 gggcggtgac gcagagtatg caggccgcga tgatgcgcgg cgacgaagcc tacagcggca
      601 gccgcagcta ctatgcgtta gccgagtcag taaaaaatat ctttggttat caatatacta
      661 ttccgactca ccagggccgt ggtgcagaac aaatctatat tccggtactg attaaaaaac
      721 gcgagcagga aaaaggcctg gatcgcagca aaatggtggc gttctctaac tatttctttg
      781 ataccacgca gggccatagc cagattaacg gctgtaccgt gcgtaacgtc tatatcaaag
      841 aagccttcga tactggcgtg cgttacgact ttaaaggtaa ctttgacctt gagggattag
      901 aacgcggcat tgaagaagtt ggcccgaata acgtgccata tatcgttgcg accatcacca
      961 gtaactctgc aggtggtcag ccggtttcac tggcaaactt aaaagcgatg tacagcatcg
     1021 cgaagaaata cgatattccg gtggtcatgg actccgcacg ctttgctgaa aatgcctatt
     1081 tcatcaagca gcgtgaagca gaatacaagg actggaccat cgagcagatc acccgcgaaa
     1141 cctacaaata tgccgatatg ctggcgatgt ccgccaagaa agatgcgatg gtgccgatgg
     1201 gcggcctgct gtgcatgaaa gacgacagct tctttgatgt gtacaccgaa tgcagaaccc
     1261 tttgcgtggt gcaggaaggc ttcccgacat atggcggcct ggaaggcggc gctatggagc
     1321 gtctggcggt aggtctgtat gacggcatga atctggactg gctggcttat cgtatcgcgc
     1381 aggtgcagta tctggtcgat ggtctggaag agattggcgt tgtctgtcag caggcgggcg
     1441 gtcacgcggc attcgttgat gcaggtaaac tgttgccgca tatcccggca gatcagttcc
     1501 cggcacaggc gctggcctgc gagctgtata aagtcgccgg tatccgtgcg gtagaaattg
     1561 gctctttcct gttaggccgc gatccgaaaa ccggtaaaca actgccatgc ccggctgaac
     1621 tgctgcgttt aaccattccg cgcgcaacat atactcaaac acatatggac ttcattattg
     1681 aagcctttaa acatgtgaaa gagaacgcgg cgaatattaa agggttaacc tttacctacg
     1741 aaccgaaagt attgcgtcac ttcaccgcaa aactgaaaga agtttaatta aaacactcag
     1801 agtggctata aggatgttag ccactctatt accctacatc ctcaataaca aaaatagcct
     1861 tcctctaaag gtggcatcat gactgatcaa tcagaaaaaa aacactctgc attttggggt
     1921 gttatggtta tagccggtac agtaattggc ggtgggatgt ttgctttacc tgttgacctt
     1981 gccggagcct ggtttttttg gggcgctttt attcttatta tcgcctggtt ttcaatgtta
     2041 cattcagggc tattattatt agaagcaaac ttaaattatc ccgtcggatc cagttttaac
     2101 accatcacca aagatttaat cggtaacacc tggaacatta tcagcggtat taccgttgcc
     2161 ttcgttctct acatcctcac ttatgcctat atctctgcta atggtgcgat cattagtgaa
     2221 acgatatcaa tgaatttggg ttatcatgct aatccacgta ttgtcgggat ctgcactgcc
     2281 attttcgttg ccagcgtatt gtggataagt tcgttagccg ccagtcgtat tacctcattg
     2341 ttccttgggc tgaagattat ctcctttgta attgtttttg gttctttctt cttccaggtc
     2401 gattactcca ttctgcgcga tgccaccagc accacggcgg gaacatctta tttcccgtat
     2461 atctttatgg ctttgccggt gtgtctggcg tcatttggtt tccacggcaa tattcccagc
     2521 ctgattattt gctatggcaa acgcaaagat aagctaatca aaagcgtggt cttcggttcg
     2581 ctgctggcgc tggtgattta tctcttctgg ctctattgca cgatggggaa tatcccgcga
     2641 gaaagcttta aggcgataat ctcctcaggc ggcaacgttg attcgctggt gaaatcgttc
     2701 ctcggcacca aacagcatgg cattatcgag ttttgcctgc tggtgttctc caacttagct
     2761 gttgccagtt cgttctttgg tgtcacgctg ggattgttcg attatctggc ggatctgttt
     2821 aagattgata actcccacgc cgggcgtttc aaaaccgtgc tgttaacctt cctgccacct
     2881 gcgttgttgt atctgatctt cccgaacggc ttcatttacg ggatcggcgg tgccggactg
     2941 tgtgccacca tctgggcggt cattatcccc gcggtgctgg caatcaaagc tcgcaagaag
     3001 tttcccaatc agatgttcac ggtctggggc ggcaatctta ttccggcgat tgtcattctc
     3061 tttggtataa ccgtaatttt atgctggttc ggtaacgtct ttaacgtgtt acctaaattt
     3121 ggctaaatcc ttcaagaagc cagccattcg ctggcttctt gcctctcagg aaatcactta
     3181 tgtccaaatg gcaactcgcc tgatcctcct tcaccacgta tgctttgcgt caccttacta
     3241 tcaggacgct ttagcccatg tcccgctttt tgatttgtag ttttgccctg gttttacttt
     3301 atcccgcagg gattgatatg tacctcgttg gtttaccgcg catcgccgcc gatctcaatg
     3361 ccagcgaagc gcagttgcat attgcgttct ccgtttatct ggcggggatg gcggctgcga
     3421 tgttatttgc cggtaaagtg gccgatcgtt cagggagaaa gcctgtcgcc atacccggcg
     3481 cggcgctatt tattatcgcc tcggtgttct gttcactggc tgaaaccagc gcgttatttc
     3541 ttgcagggcg atttctacag ggactgggcg cgggctgttg ttacgtagtg gcgttcgcca
     3601 ttttgcgcga cacgctggat gatcgacgtc gggctaaagt gctgtcgtta ctcaacggca
     3661 ttacctgcat cattccggtg ttagcgccgg tgctcggaca cctgattatg cttaaattcc
     3721 cttggcagag tctgttctgg acgatggcaa cgatgggcat cgcggtactg atgttgtctt
     3781 tgtttatttt aaaagaaacg cgccctgcgg cccccacaac ttcggacaaa ccacgagaaa
     3841 atagcgagtc actgcttaat cggtttttcc tcagccgtgt tgttatcacc accctcagcg
     3901 tttcggtgat cctcaccttc gtcaacacgt cgccagtatt gctgatggaa atcatgggtt
     3961 ttgagcacgg agaatacgcc accattatgg cgttgaccgc tggcgtcagc atgaccgttt
     4021 cattctccac gccgtttgcg ctgggaattt ttaagccacg tacgttgatg atcacctcac
     4081 aggtgttatt cctggcggcg gggatcaccc ttgccgtttc accttcccat gcggtttcgc
     4141 tgtttggtat cacgctgatt tgcgccggtt tctcggtagg ttttggtgtg gcgatgagtc
     4201 aggcgttagg gccgttttca ttacgcgcgg gcgtagccag ctcgacctta ggtattgcgc
     4261 aagtttgcgg ttcgtcactg tggatttggc tggcggcggt ggttggtatc ggcgcatgga
     4321 atatgctgat cgggattctg attgcctgta gcatagtgag cctgttgctg attatgttcg
     4381 tcgcgcctgg acgcccggtt gccgctcatg aagaaatcca tcaccacgct tgatctcaat
     4441 ctgctgctct gtctgcaact gctgatgcag gagcgcagcg tgaccaaagc ggcgaagcgg
     4501 atgaacgtga ccccttcggc ggtgagtaag tcgctggcaa agttaagagc gtggtttgac
     4561 gacccgctct ttgtgaactc accgctgggg ctgtcgccca caccgctaat ggtcagcatg
     4621 gagcaaaatc tggcggagtg gatgcaaatg agcaacctgc tgctggataa accgcaccac
     4681 cagacaccgc gcggcctgaa gtttgagctg gcagcagaat caccgctaat gatgatcatg
     4741 cttaatgcgc tgtcgaaacg gatctaccaa cgttacccgc aggcgaccat caaattacgt
     4801 aactgggatt acgattcctt agatgccatt actcgtggtg aagtggatat tggtttttcc
     4861 ggtcgcgaaa gtcatccccg ttcgcgggag ctgttaagct cgctaccgtt agccattgat
     4921 tatgaagtgc tgtttagtga tgtgccctgc gtctggttac gcaaagatca tccggcactg
     4981 catgaagcat ggaatctgga caccttttta cgttatccgc atatcagcat ttgctgggaa
     5041 cagagcgata cctgggcgct ggacaatgtg ttacaggagc tggggcgcga acgcacgatt
     5101 gctatgagcc tgccggaatt cgagcagtca ctgtttatgg cagcacagcc cgacaatctg
     5161 ctactggcga ccgcaccgcg ctactgtcag tactacaatc aactccacca actgccgttg
     5221 gttgctcttc ctctcccgtt tgacgaaagc cagcaaaaaa agctggaagt cccttttacc
     5281 ctgctgtggc ataaacggaa cagccataat ccgaagatcg tctggttacg ggaaaccatt
     5341 aaaaatcttt acgcgtcgat ggcataaccg aatcgtatga aacgcgaccc aatttcacaa
     5401 taaaatgtaa aaaagttgta ataagcttgt ctgaatcgaa cttttagccg ctttagtctg
     5461 tccatcattc cagtaaatga ttactcttgt attcataatg gaccattaag catggagcga
     5521 aaaatggcga ctcactttgc ccgagggatt ttaacggaag gacatctgat ttctgttcgt
     5581 ctcccctccc agtgtcatca agaagcccga aacattcccc ctcatcgtca aagccgtttt
     5641 ctggcgtcca gaggtttact cgcagaactg atgttcatgc tgtatggcat tggcgaattg
     5701 ccggaaatcg tcaccctgcc gaaaggaaaa ccggttttca gcgataaaaa tttgccttcg
     5761 ttttctattt cctatgccgg gaatatggtc ggcgtggcgt taacaaccga aggtgaatgt
     5821 ggcctcgata tggaactaca gcgtgcgacg cgcgggtttc atagcccaca cgcgcccgat
     5881 aaccacacct tttccagcaa tgaatcgcta tggatcagta aacaaaacga tcctaacgaa
     5941 gcgcgggcgc agctcatcac gctgcgccga agcgtgctaa aactaaccgg tgatgttttg
     6001 aatgacgatc cgcgcgatct gcaactactg cccattgccg gacgcctgaa atgtgctcat
     6061 gtaaatcatg tagaagcgtt atgcgacgcg gaggacgtgc tggtgtggtc cgtggcggtc
     6121 acgcccacga ttgaaaagct cagtgtctgg gagttagatg gcaaacatgg ctggaaaagc
     6181 ctgcccgata ttcacagccg cgccaacaat cctaccagcc ggatgatgcg ttttgcccag
     6241 ctctctaccg tgaaggcttt ttcgccaaat tgatagacaa ccacaggagt catcatgtct
     6301 gaaaaattgc aggtggttac gttactgggg agcctgcgca aaggctcatt taatggcatg
     6361 gttgcacgta ccctgccgaa aattgctccg gcgagcatgg aaatcaatgc gttaccttcc
     6421 attgccgaca ttcccctgta tgacgctgac gtacagcagg aagacggttt tccagcaaca
     6481 gttgaagctc tggcggaaca gatccgtcag gctgacggtg tggtgatcgt cacgccggaa
     6541 tataactact cggtaccggg tgggctgaaa aatgccatcg actggctttc ccgcctgccg
     6601 gatcaaccgc tggctggtaa accggtattg attcagacca gctcaatggg cgtgattggc
     6661 ggcgcgcgct gtcagtatca cctgcgccag attctggttt tcctcgatgc aatggtgatg
     6721 aacaagccgg aatttatggg cggcgtgatt cagaacaaag ttgatccgca aaccggggaa
     6781 gtgattgatc agggtacgct ggaccacctg accgggcaat tgaccgcatt tggtgagttt
     6841 attcagcgag ttaagatct
//
LOCUS       DAZKGF010000221         6825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZKGF010000221 DAZKGF010000000
VERSION     DAZKGF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.221"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..152)
                     /locus_tag="ACHOQV_004553"
     CDS             complement(<1..152)
                     /locus_tag="ACHOQV_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632822.1"
                     /translation="MLQSSADKACNVLVKKVRYLNDVRKITKMMNGKLIYSWLVWPCS
                     EFFSLN"
     gene            230..1324
                     /gene="ddlA"
                     /locus_tag="ACHOQV_004554"
     CDS             230..1324
                     /gene="ddlA"
                     /locus_tag="ACHOQV_004554"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9632823.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1527..1739)
                     /gene="yaiZ"
                     /locus_tag="ACHOQV_004555"
     CDS             complement(1527..1739)
                     /gene="yaiZ"
                     /locus_tag="ACHOQV_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFP9632824.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1999..2307
                     /gene="yaiY"
                     /locus_tag="ACHOQV_004556"
     CDS             1999..2307
                     /gene="yaiY"
                     /locus_tag="ACHOQV_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFP9632825.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2366..3460)
                     /gene="yaiW"
                     /locus_tag="ACHOQV_004557"
     CDS             complement(2366..3460)
                     /gene="yaiW"
                     /locus_tag="ACHOQV_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFP9632826.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATMFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3473..4693)
                     /gene="sbmA"
                     /locus_tag="ACHOQV_004558"
                     /pseudo
     CDS             complement(3473..4693)
                     /gene="sbmA"
                     /locus_tag="ACHOQV_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
     gene            5045..6202
                     /gene="ampH"
                     /locus_tag="ACHOQV_004559"
     CDS             5045..6202
                     /gene="ampH"
                     /locus_tag="ACHOQV_004559"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFP9632827.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRVQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6203..>6825)
                     /gene="iprA"
                     /locus_tag="ACHOQV_004560"
     CDS             complement(6203..>6825)
                     /gene="iprA"
                     /locus_tag="ACHOQV_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFP9632828.1"
                     /translation="LSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDTF
                     VILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGSCAGYHLPAKQTI
                     TLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRIG
                     VMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
BASE COUNT         1740 a         1759 c         1642 g         1684 t
ORIGIN      
        1 taattaagag aaaaaaactc actgcatggc catacaagcc aggaataaat tagcttcccg
       61 ttcatcattt ttgtgatctt gcgcacatca ttgagataac gcactttctt caccagtacg
      121 ttgcacgctt tgtcagcaga agattgcaac attctgtcag aaggccgcga atcccccgtt
      181 ttgtcgtggt aatgtatgca ctttgtgtta gacgggatag gtttttaaga tggaaaaact
      241 gcgggtagga atcgtttttg gtggtaaatc agcggaacat gaagtgtctc tgcaatcggc
      301 aaaaaacatt gtcgatgcca ttgataaaag tcgcttcgac gttgtgctgc tgggcattga
      361 taaacaaggg caatggcacg tcagcgatgc cagcaattat ctgctaaatg cagacgatcc
      421 tgcccatatt gcgttgcgcc cttcggcgac cagccttgcg caagtgccag gtaaacatga
      481 gcatcagctt atcgacgcgc aaaacggtca gccgttgccg acggtggatg tcattttccc
      541 gattgtccac ggtacgctgg gcgaagatgg ttccttgcag ggaatgctgc gggtcgccaa
      601 tttaccgttt gtaggttctg atgttctggc ttcagcagcc tgtatggaca aagatgtcac
      661 caaacgtctg ctgcgcgatg ccgggctgaa cattgcgcca tttattaccc tgacgcgcgc
      721 taatcgtcac aacatcagtt ttgccgaagt ggagtctaaa ctggggttac cgctgtttgt
      781 aaaaccggct aatcagggct cttctgttgg tgtcagcaaa gtaaccagcg aagaacagta
      841 cgcaattgcc gtcgatctgg cgttcgagtt cgatcataaa gtgatcgttg agcaagggat
      901 aaaaggtcgt gagatcgaat gcgcagttct gggcaacgac aatccgcaag ccagcacctg
      961 tggcgagatc gtactcacca gcgatttcta tgcctacgac accaagtaca ttgacgaaga
     1021 tggcgcgaaa gtggtagttc cggcagccat tgcgccagaa atcaacgata agatccgggc
     1081 gattgccgtt caggcttatc agacgttggg atgcgcaggc atggcgcgtg tagacgtgtt
     1141 tttaacccca gagaacgaag tggtgatcaa cgagatcaac acactgcctg gcttcaccaa
     1201 catcagtatg tatccgaagc tgtggcaagc cagcggtctg ggttacaccg acctgatcac
     1261 gcgtttgatt gagctggcgc tggagcgtca cgctgcggat aacgcattga aaaccacaat
     1321 gtaattttta aatgcctgat aacttttatt gggcatttaa gactgatggc aaacgtaaga
     1381 ttgcctgatg cgctttgctt atcaggccta ccagataatt gcaatatact gattttgcat
     1441 gattttgtag gcagggtaag gcgttcgcgc cgcatccggc aaaaacaacg agcactttgt
     1501 cagcccccag aacgcccaat cctctgttat tgggcgtttt cagtttcttc atcgcgacga
     1561 cgaataatca gccccgccag ccagaaacta atcacccacg tcaccagacc gacggcatag
     1621 gtctgccaac cttttgcttc aaatcccagt aaacccacca cgccattaag aatgaatata
     1681 aggccaatgg cgaacgcata gtagtgccag tcacggcgga tttttacagg caagttcatg
     1741 gcagctccgt taacgaaaaa caacatcctt gcatattctg caacatccgt ctgtggcgta
     1801 gacaacattt ccgtaattgt taataaattt aacttaaggt tgtcattttg tgactcacga
     1861 cgaagattca cgcttcggga atattcccaa catcaattta ccatgattct gatattgaca
     1921 atcttaatga tctgtcagac ttgccataag ccgctggtat cacaccagaa gagaagcaga
     1981 tgacttccgg aggtttttat ggctgatttc accctgtcaa aatcgctgtt tagcggaaaa
     2041 tatcgcaatg cctcttcaac gcctggcaac attgcctatg cgttgtttgt gctgttttgc
     2101 ttttgggctg gggcgcaatt gctgaacctg ttagtgcatg cgcccggcgt ctatgagcgc
     2161 ttaatgcagg tccaggaaac aggtcgccca cgggtggaaa ttggtttagg tgtcggcacc
     2221 attttcgggc tgatcccgtt tttagttggc tgcctcattt ttgcagtagt ggcgctatgg
     2281 ctgcactggc gacatcgccg ccagtaagat ttacgttgcc agatgcggcc taaaacgcct
     2341 tatctggcat gacattcctg tttgatcact gtttcataca acgcgcccgc cgttcgtcta
     2401 cgcgcttcgc aaaccaggcc gtagtcaggt tgcgggtgat tttcgggctt tccagttgaa
     2461 tgccaggcaa catttcgcgg ggtagtgatt tacccgtttt cgcttcggca agttgataaa
     2521 ctttcttgta cagcgccgtc tcctcaaaag agaagctgtc ccctttctct aactggcggc
     2581 gaatttcact gtcgctcatc cccagttttc ccgccagttt gcgcgttgcc agttcggttt
     2641 tcccgggctc tttactgtcg taacgaatta aatcaccgtc cagtgccagc ttcacgccgc
     2701 tggccttact gaccgcgttc tgaaacgcgg cattacggct ggcgtaccag ccagcgttaa
     2761 aatcagcaaa acggtatatc ggcgcgctat agctggcggg ataattcagt aaatggtaag
     2821 taccaaacca caacccgccg cgacgactga agacttcctg acggactgta ccgtccattt
     2881 tccacggata ccctttggta tgctgttcgg caaaggcaat gctgacctgc atcggcccac
     2941 cggtgcgcac cggattaagc gaaccaaaca acgtctgccc cattggcacc atgctgatca
     3001 gatcgtcaaa aatcgcgctc aattgctttt cagtacgcac cgaatctaac cgttcgctat
     3061 agcttttgcc gtttggcgat ttgattttta gtgcggtatg caccagaaaa gcaggaatgt
     3121 gcatccgttc agcacgacgg tcaatctctt gccaggcgat cttgcttaag cccggaacag
     3181 ccggatctgc ctgataattt gactcctgct gcgccaccgc cagcaccgag cagacgtttt
     3241 ccagcgtcgg tgccagcccc tgactttcaa acatggtggc gagatctttc gcccaggcat
     3301 cgcggtcttt cacgctcgca ggcattttct gccgcacgac gctcgccaca tctatcgctt
     3361 tttcgccctt tttcaacggt tgcggagcct gactactaca tcccgctaaa accagaaccg
     3421 ccaacaatga cagcgaagat agatgattga cgcgcgacat tacttctcct ttttagctca
     3481 aggtatgggt tacttcctga attttgtcac catccagctc atgttcaaag ctgcgcagac
     3541 gtttgtagat agacatcaac tcaaccagtg tggtccatga gttaatcagg tactggaaag
     3601 caccgcgaac ctgaccaaaa acgttagtaa tctgcgtcat caggccgagt gtaatcgtac
     3661 cggcaacaat tgacggaaac agcaagaaca aaccgaaaac gttatcgacc tgcaaataga
     3721 gaatgcgggc gatgttgaaa tacatatagt gaaaatagag gcggaaatag tttttccgta
     3781 cggcgctaaa cagctcgcgt accgtaggcg gcgtcgcacg cgtggcatcg tcttcaccat
     3841 aaaccagctc tttacggtag gcagcctcta ctcgctggtt tttaaactcc agccccggca
     3901 gtttgatccc taccactgcc agtaatccgg tccccatcag cgaccagacg atagcggcaa
     3961 tcaccagacc atacggaatg tgcccgacaa tcggcagctc cggcacatgt gcggagagcg
     4021 ttaccagcac cggcaggaag gcgatcaacg tcatgatagc gttgataaag ctgacgccca
     4081 tattctccag cgttgaagca aaacgcatgg tgtcttcctg cacacgctgc gcggcccctt
     4141 cgatatgacg cagttgttgc cagttcgcca tgtaatattc gttcatcgcc gtacgccagc
     4201 ggaacacgta gtgactgaca aagaagttgt tcagcacact gatcaccacc gcgatcagcg
     4261 caatccccag aaagactccc acttcgcggt aaaattgttc gatggtgact ttatgcggcg
     4321 aacttagcgc cgtttgaatc agatcataga acggcgcata ccaggcgttg acggcaaccc
     4381 cgacttccac caaaaaccag gtgacgaaga taatcagcgc agtaccgagt attgaccagt
     4441 attgccaacg gtgcgggcta tagataaacc agaacaatgc aaaaagtcct acgcaaacaa
     4501 tgtagtaagc gtaaaaaatc aggaaatcca acgaccagaa acgcgcggcg ctaatcggga
     4561 tctgcccgga agcgccggtg atacgcgcca cccaatcccc cccaccggct taccagaaga
     4621 taacggcaat caatgcccaa acaaaggccg agagaaaaaa cgttcccggc tttgggaaaa
     4681 aagacttaaa catatgcact cctgctaact tcttatcgtt ttaacaactg ctgtgtatta
     4741 accgcatgac cgctacaaag cgtcagccaa atgaaaccgc ccattgtgac ccatagcaag
     4801 gcgcttagtt cgccagcctg agcataagaa ttgtttcgac ggatttcaca aaccaaagat
     4861 tgtttacata agcggattaa cacgcgcagg atcacaaatg caggcattgg tgagcaggaa
     4921 aaagatcgtg tttacgcgcc gttacgtttt taacactatt attgcactaa ttcgcccttt
     4981 gcaatccatc aatgagtagt ataaatacgc tcagttacct tcattcaatc tatggacacc
     5041 accgttgaaa cgtagtctgc ttttttctgc cgtgctgtgt gcggcgtcat tgacctccgt
     5101 ccatgcggcg cagccgatca ctgaaccgga gttcgcctct gatattgtcg atcgttatgc
     5161 cgatcatatt ttttacggca gcggtgccac ggggatggcg ctggttgtta tcgacggtaa
     5221 ccagcgcgtc tttcgcagtt atggcgaaac gcgacctggt aataacgttc gcccgcagct
     5281 ggattcggtc atacgtattg cttccctcac caagctaatg accagtgaga tgctggtgaa
     5341 attgctcgac caggggaccg tgaagttaaa cgatccgcta agtaaatatg ctccgccagg
     5401 cgcacgcgta ccgacttaca atgggacgcc cattacgctg gtgaatctgg caacccatac
     5461 cagcgccctg ccccgtgaac agcccggtgg cgcggcacat cgtccggtat ttgtctggcc
     5521 aacgcgcgag caacgctgga aatacctttc tacggcgaag ctgaaagccg cgccaggcag
     5581 tcaggcggcg tactctaacc tcgcattcga cctgctggca gatgcactgg cgaatgcgtc
     5641 cggcaagcct tatacccagt tgtttgaaga gcagattacc cgtccattgg ggatgaaaga
     5701 caccacctac accccctcac cggatcagtg ccgccgttta atggttgctg agcgcggtgc
     5761 cagtccgtgc aataacacgc tggcggcaat tggcagcggc ggcgtctatt ccacgccagg
     5821 cgatatgatg cgctggatgc agcagtattt gtcatctgat ttttatcagc gcagtaacca
     5881 ggctgatcgc atgcagacgt tgatttatca gcgcgtgcag tttacaaaag tgattggcat
     5941 ggatgtcccc ggcaaagcgg atgcgcttgg tttaggctgg gtatatatgg ccccgaaaga
     6001 gggtcgtcca gggattattc agaagacagg tggtggtggc ggtttcatta cctatatggc
     6061 gatgatcccg cagaagaaca ttggcgcatt tgtggtggtc actcgttcgc cattgacgcg
     6121 cttcaaaaat atgagcgatg gcatcaatga cctggtgacc gagctaagcg ggaataaacc
     6181 gttggttatc cccgcatcct gattaatact ctgaaggcaa acggttgata gcgaccagtt
     6241 tgcctttatt catttcgata tagccgcctt tacgcaaagc agcgagaact tccgcaacga
     6301 cagaacgcga tatacgtgta cgttgatgga tataattcat cacaccaata cgtgatcgca
     6361 attcttcatt ccagtcaatc attgataata acgtcgcgcg gatttgttcg taggaattat
     6421 gcccaatgag ctgaacgtcg cgtaattcca gaattctatt ttgccaggct aaccagtaaa
     6481 aagcgtctcg ccagagttga ttttgttcaa taagagtaat ggtttgtttg gctggtaaat
     6541 gatatcccgc acagcttcct tctgatatta atttgtatgg tatatcgttt ttcattaaac
     6601 catcagccag ccccataata taaggagcct gggtaatacc gataagtacg ttttcttctc
     6661 tacgcagaga gataacgccc tctaaaataa cgaaagtatc ttcgctattg acatcactgg
     6721 aaaatataac ttgcttttca ttattaaact cgaagcgcgt accgtatctg gacaaacatt
     6781 tatcgagctt accaaattcc tgaagaggtt taactacaga taaca
//
LOCUS       DAZKGF010000222         6589 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZKGF010000222 DAZKGF010000000
VERSION     DAZKGF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.222"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(167..2200)
                     /gene="betT"
                     /locus_tag="ACHOQV_004561"
     CDS             complement(167..2200)
                     /gene="betT"
                     /locus_tag="ACHOQV_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFP9632829.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2329..2916
                     /gene="betI"
                     /locus_tag="ACHOQV_004562"
     CDS             2329..2916
                     /gene="betI"
                     /locus_tag="ACHOQV_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFP9632830.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKALDKPLAHSLTRHFITQHLPTD"
     gene            2930..4402
                     /gene="betB"
                     /locus_tag="ACHOQV_004563"
     CDS             2930..4402
                     /gene="betB"
                     /locus_tag="ACHOQV_004563"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFP9632831.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFIPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRFIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4416..6104
                     /gene="betA"
                     /locus_tag="ACHOQV_004564"
     CDS             4416..6104
                     /gene="betA"
                     /locus_tag="ACHOQV_004564"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFP9632832.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GAECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDAEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGQEALPRSTARYFVANGMPVRAKKMSRDVN"
BASE COUNT         1610 a         1808 c         1797 g         1374 t
ORIGIN      
        1 cagtccaaac gtaggttttg ggcttcaata gttcttttct tttttattat ctgttgcctg
       61 gcaatcatca atctatacta agggcttaac tgtacagata taacgtatca agaggtaaga
      121 gaaaccgcgc catatgcata catggcgcgg ttttatgcaa taacaatcac gcgtccggga
      181 acatcacgct atggcccggc gcttcacgat ggagatgaat aaagttaagg tgccgctcgt
      241 actggtcaag aatatcggtg atcacctgct ctttgctgta gtccatcagg tcgttgccct
      301 ggctgccttc taacaggaag gtttccagcc ggtagtaggt cgatttaccg ctacgtgcgc
      361 ggtaggtaaa gcccggcacc gaatattgct gcggccaaat ctgatagaca aagttttgtt
      421 cttcgcccat atgcaccaac aaatccagat gccccaactg ttgtccctct tccggcggca
      481 ggctttttag ctccacgtat gcgccgcgca accgcagctc ctgcgccact tcttccattg
      541 ccgggtaaca gaccgtctcc atcatctgtt tagtgtaacg cgtgcccgga taattcatca
      601 ggcgcgagag acgttttttc cagctcaggc gatcctgaag ccccagcggt cgcggcgcgg
      661 tatcgcggtt cgcactttca cggcggtaat cttctacctt cagagattta tacaaccccg
      721 ccatcacgaa gaaaatcaca aagctgaacg gcagccccat gattaccgtg gtgttttgca
      781 gcgcggatat cccgttggtc atcagcatgc cgagcgtcag caggccaatc gccaccgacc
      841 agaagacgcg cagccagccg ggggcgtcgc tgttgatatc tttaagctgc gaggtgaaat
      901 tccccagcac cagcgcaccg gagtccgccg aggtcacata aaacagcagg ccagtaatgg
      961 tggcgacgga ggcgctaaag gtaaacgccg gatattgcgc cagcaggctg tagaagccgc
     1021 gctccggatg gaccatcgct tcctcggcaa atgccgcgcc gccgtggatg atttcataca
     1081 gcgcgctatt gccgaatacc gagagccata acagcgtgaa ggtaaacgga ataatcaacg
     1141 tgcccagcac gaactggcga atggtacgcc cacgcgagat acgcgccagg aacaagccga
     1201 caaacggcga ccatgccacc caccatgccc agaagaagag cgtccagtta ttcatccact
     1261 caaccggacg gtcgaaggca aaactgttga gcgtcatgcc cataaagcga ttcacatagt
     1321 cgccaacatt cagcaccagt gcattaagca ggaacgaggt gtcgcccata aacaacacga
     1381 acaggatcaa tcccagcgcc agcgcaacgt tgagttccga taacacgcga atgcccttat
     1441 caacaccgga tgtcaccgag atagtggcga tgatcaccga caaggcgatc agtgccgctt
     1501 tcgccgccat cgaatcggga atatcaaaca gtacgctcaa gccatagtta agctgcacca
     1561 caccgatacc gagcgtagtg gcaataccga agatggtgcc gatcaccgct gcaatatcca
     1621 ctgaatgacc aatcggcccg ttaatccgtt taccgaagat cgggtacagc gccgagcgga
     1681 tggtgagcgg caaattataa cgataactaa agtatccgag cgccatgccc atcagcgcat
     1741 acatcgacca gccggttaag ccgtagtgaa acagcgtcca gaccatcgcc tgacgcgcgg
     1801 cctcaatcgt ctgtcccgcg ccttccggcg gctgcatata ctgcgttacc ggttcggcta
     1861 cggagaagaa catcaggtcg ataccgatcc cggcagcaaa cagcatcgcc gcccaactca
     1921 gcagactgaa ttccggtttg gattgttctg gcccgagctt caccgaacca aaacgcgaac
     1981 aagcgataca gaccacaaag acaatataga gcgttgccgc cagcagatag taccaaccga
     2041 aggttttaga aacccagtcc agcgtgcggc caatccacag ggccgagaag tcgcgaaaca
     2101 ggatcgttgt cagggaaaac aacaaaatca gtccggcgga ggtgtaaaac accaccggat
     2161 tgattttgtc cttttccctg ctgtgtgaaa ggtctgtcat ccagtatccc cactgttatt
     2221 gttaccattt aaaatcaaat tcgtaacaat taagacacat tttatattga acgtccaatc
     2281 aataaccgct ttaatagata aacaacgctg atgaatggag tggcgaaaat gcccaaattg
     2341 gggatgcagt cgatccggcg cagacaactg atcgacgcca cgctggaagc aataaatgaa
     2401 gtgggcatgc acgatgcaac gatcgcgcag atcgcccgcc gtgcaggcgt ttctacgggg
     2461 atcatcagcc actatttcag ggacaaaaat ggtctgctgg aagcaaccat gcgcgatatc
     2521 accagtcagc tgcgtgacgc ggttttgaat cgattacatg cacttccgca gggcagtgca
     2581 gagctgcgat tacaggcgat tgttggtgga aacttcgatg aaacccaggt gagcagtgcg
     2641 gcgatgaaag cctggctggc cttctgggcc agcagtatgc atcagccgat gctctatcgc
     2701 ttgcagcagg tcagtagccg ccgcttattg tcgaatctgg tgagcgaatt tcgccgcgag
     2761 ttaccgcgcc aacaggcaca ggaagcgggc tacggccttg ccgcgctgat tgacggatta
     2821 tggctgcgcg cggcactgag cggcaaagcg ctggataaac ctctcgcaca ctcgctgacc
     2881 cgtcacttta ttactcagca tttacccacc gattaaccga ggaaacgtga tgtcccgaat
     2941 ggcagaacag cagctttata tacatggtgg ttatacctcc gccaccagcg gtcgcacctt
     3001 cgagaccatt aacccggcca acggtaacgt gctggcgacc gtgcaggccg ccgggcgtga
     3061 ggatgtcgat cgcgccgtga agagtgccca acaagggcaa aaaatctggg cggcgatgac
     3121 cgctatggag cgctcgcgta ttctgcgtcg ggccgttgat attctgcgtg aacgcaatga
     3181 cgaactcgca aaactggaaa ccctcgacac cggaaaagcg tattcggaaa cctcaaccgt
     3241 cgatatcgtt accggtgcgg acgtgctgga gtactacgcc gggctgatcc cgtcgctgga
     3301 aggcagccag atcccattgc gtgagacgtc atttgtttat acccgccgcg aaccgctggg
     3361 cgtagtggca gggattggcg catggaacta cccgatccag attgccttgt ggaaatccgc
     3421 cccggcgctg gcggcaggca acgcgatgat tttcaaaccg agcgaagtca ccccgcttac
     3481 cgcgttaaag ctggccgaaa tttacagcga agcgggcctg ccggacggcg tatttaacgt
     3541 gctgccgggc gtaggtgcgg aaaccgggca gtatctgacc gagcatccgg gcattgccaa
     3601 agtgtcattt accggcggtg tcgccagcgg caaaaaagtg atggctaact cggcggcttc
     3661 ctccctgaaa gaagtgacca tggagctggg cggtaaatca ccgctgatcg ttttcgacga
     3721 tgcggatctc gatctcgccg ctgatatcgc catgatggcg aacttcttca gctccggtca
     3781 ggtgtgtacc aacggcaccc gcgtgtttat tccggcgaaa tgcaaagccg catttgaaca
     3841 aaagattctg gcgcgcgttg agcgcattcg cgcgggcgac gttttcgatc cgcaaactaa
     3901 ctttggcccg ctggtcagct tcccgcatcg cgataacgtg ctgcgttaca tcgccaaagg
     3961 caaagaggaa ggcgcgcgcg tactgtgcgg cggcgatgta ctgaaaggcg atggcttcga
     4021 taacggcgca tgggttgcac cgaccgtgtt caccgattgc cgcgacgaaa tgaccatcgt
     4081 gcgcgaagag atcttcggtc cggtgatgtc gcttctcact tatgaatctg aggacgaagt
     4141 gattcgccgc gccaatgata ccgactacgg cctggcggcg ggcatcgtga cggcggacct
     4201 gaaccgcgcg catcgcttca ttcatcagct ggaagcgggt atttgctgga tcaacacctg
     4261 gggcgaatcc cctgcagaga tgcccgttgg cggctacaaa cactccggca ttggtcgcga
     4321 gaacggcgtg atgacgctcc agagttacac ccaggtgaag tccatccagg ttgagatggc
     4381 taaattccag tccatattct aaccaggagg tttatttgca atttgactac atcattattg
     4441 gtgccggctc agctggcaac gttctcgcta cccgtctgac tgaagatccg aacacaaccg
     4501 tgctgctgct tgaagcaggc ggcccggact atcgctttga cttccgcacc cagatgcccg
     4561 ccgcgctggc gttcccgtta cagggtaaac gctataactg ggcctatgag acggaaccgg
     4621 aaccgtttat gaacaaccgt cgcatggagt gcggacgcgg taaggggctt ggcgggtcat
     4681 cactgatcaa cggcatgtgt tacatccgtg gtaacgcgct ggatctcgat aactgggcga
     4741 aagaacccgg tctggagaac tggagctacc tcgactgtct gccctactat cgcaaggccg
     4801 agactcgcga tgtgggcgag aacgactacc acggcggtga tggcccggtg agcgtcacca
     4861 cctccaaacc cggcgtcaat ccgctgtttg aagcgatgat tgaagcgggc gtgcaggcgg
     4921 gctacccgcg cacggacgat ctcaacggct atcagcagga aggttttggc ccgatggatc
     4981 gcaccgtcac gccgcagggc cgtcgcgcca gcaccgcgcg tggctatctc gatcaggcca
     5041 aatcgcgccc taacctaacc attcgtactc acgccatgac cgatcacatc atttttgacg
     5101 gcaaacgcgc ggtgggcgtc gagtggctgg aaggcgacag caccattccg acccgcgcgg
     5161 cggcgaataa agaagtgctg ttatgtgcag gcgcgattgc ttcaccgcag atcctgcaac
     5221 gctccggcgt cggcaacgct gaactgctgg cggagtttga tattccgctg gtgcatgatt
     5281 tacccggcgt cggcgaaaat cttcaggatc acctggagat gtatctgcaa tatgagtgca
     5341 aagaaccggt ttccctctac cctgccctgc agtggtggaa tcagccgaaa atcggtgcgg
     5401 agtggctgtt tggcggcacc ggcgttggtg ccagcaacca ctttgaagca ggcggattta
     5461 ttcgcagccg tgaggaattt gcgtggccga atattcagta ccatttcctg ccagtggcga
     5521 ttaactataa cggctcgaac gcagtgaaag agcacggctt ccagtgccac gtcggatcga
     5581 tgcgctcgcc aagccgtggg catgtgcgga ttaaatcccg cgacccgcac cagcatccag
     5641 cgattctgtt taactacatg tcgcacgagc aggactggca ggagttccgc gacgcaattc
     5701 gcatcacccg ggagatcatg catcaaccgg cgctggatca gtatcgtggc cgcgaaatca
     5761 gccccggcgc ggaatgtcag acggatgagc agctcgatga gttcgtgcgt aaccacgccg
     5821 aaaccgcgtt tcatccgtgc ggtacctgca aaatgggtta tgacgagatg gcagtggttg
     5881 acgccgaagg ccgcgtacac gggctggaag gcctgcgcgt ggtggatgcg tcaattatgc
     5941 cgcaaattat caccggcaat ttgaacgcta cgacgattat gattggcgag aaaatggcgg
     6001 atatgattcg cgggcaagaa gcgttgccga ggagcacggc aagatatttt gtggcaaatg
     6061 ggatgccggt gagagcgaaa aaaatgagtc gtgatgtgaa ctaactcagg aacctatcag
     6121 attgagagtt accgttctac tatgaggacc gaatccttat ataaaacact gaggtaactc
     6181 tcatgcttca tatccgcagt cccgccatca aactttaagc cgattcgctc aatctggcgg
     6241 atgatggtgc tatggatacg ctgataaacc acaagtgtaa tgccactcag cttaagaatc
     6301 acgcaaccag tgctcctggc tagggttact tgttctttcc cactcccggt tagcagtggt
     6361 cagcgatcaa ctgcgtaagc cgggcgtatt tatcccaaag cgtggagtaa tatcaggtct
     6421 acgctaaaaa agtcaattta ttcggttatc atgcacgaaa aggggacttg tggcgagtat
     6481 aaaaataagt cagccccaaa aactacgtgg agctttaaac acggttcgta taatttatta
     6541 gcagcgattt atttttaccc ttctttaatc agtcatcatg attaaatat
//
LOCUS       DAZKGF010000223         6447 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZKGF010000223 DAZKGF010000000
VERSION     DAZKGF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.223"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..115
                     /locus_tag="ACHOQV_004565"
     CDS             11..115
                     /locus_tag="ACHOQV_004565"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9632833.1"
                     /translation="MFTVTAATEREARAQLPHAHLIFVARIRQEVTHA"
     gene            108..293
                     /locus_tag="ACHOQV_004566"
     CDS             108..293
                     /locus_tag="ACHOQV_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632834.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            297..491
                     /locus_tag="ACHOQV_004567"
     CDS             297..491
                     /locus_tag="ACHOQV_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632835.1"
                     /translation="MCNFSDMTVDEAAKSAMQAELICSLMMGNTGEMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDMQ"
     gene            558..671
                     /locus_tag="ACHOQV_004568"
                     /pseudo
     CDS             558..671
                     /locus_tag="ACHOQV_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            677..2497
                     /locus_tag="ACHOQV_004569"
     CDS             677..2497
                     /locus_tag="ACHOQV_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFP9632836.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWLEQDGKLA
                     GDNIPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHSERQNWRNYLDIERKNISDGLVIELPVKKKARGQGDDSSLALNQMGASQ
                     RGEVLLAHYGGELAINADSDTVHYYNSVVWVPVQDKELQRTMAQIFIDEDICYSQNAI
                     KSAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGDFREHDKNDWLLIASELPFTPPAE
                     GETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKS
                     VMAEICTMLAGKANTVSASMKALEDARERALVVGFSLIILPDMTRYAGDGAGIKAITG
                     GDKVAIDPKHKAPYSTRIPAVVLAVNNSAMSFSDRSGGISRRRVIFNFSEVVPENERD
                     PMLPEKIEGELAVVIRHLLTRFSDQDEARLLLHEQQKSEEALAIKREGDSLVDFCGYL
                     MSSVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFSKPVTLTRFGADMPGAMAEY
                     GREYMKRKTKHGLRSNVTLTEESEDWMPSCAPVKNEGDKN"
     gene            2784..3233
                     /locus_tag="ACHOQV_004570"
     CDS             2784..3233
                     /locus_tag="ACHOQV_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632837.1"
                     /translation="MTEKHAKKSHSHAIDLTEPESVVRFVSVRGRATLYIPDEHLHHC
                     DKHKLPILIVWKRTVYADVTWLNDSLMLIHRDLFEREEFRRDIEERAGKIYEKYAANS
                     KRAARAITYHFMTLYDLKADDAEKAACDLFDMTMGIIQEYRNKERRP"
     gene            3230..3679
                     /locus_tag="ACHOQV_004571"
     CDS             3230..3679
                     /locus_tag="ACHOQV_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9632838.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTAMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            3696..4142
                     /locus_tag="ACHOQV_004572"
     CDS             3696..4142
                     /locus_tag="ACHOQV_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFP9632839.1"
                     /translation="MNQQTTQQDQTTRKPSRRIPWQHMTKVQRKNHRRINRIAEYWPE
                     LFGTENIKPLKAGIIRDVLEDAKSRELPLATGVLRGALTSHLHTTRYLKAIIAGGPRY
                     DLNGQPCGEVTEEDKSVASELLKRRLAQIKQQNSQQQTTGDDGDDS"
     gene            4132..4425
                     /locus_tag="ACHOQV_004573"
     CDS             4132..4425
                     /locus_tag="ACHOQV_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9632840.1"
                     /translation="MIADSVAQKLEERGLWRRAATRWGDVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQLVTFGDVKKAADRTLKEMGINAQDEWKNYSFSDAGDDLALP"
     gene            complement(4862..5059)
                     /locus_tag="ACHOQV_004574"
     CDS             complement(4862..5059)
                     /locus_tag="ACHOQV_004574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632841.1"
                     /translation="MIGFVIGFVIDLRIFLRNFLCIFSGIFPWIFHPTEGASGLPDND
                     QVNRTGKGAGCAGGRAIKNPE"
     gene            5336..5549
                     /locus_tag="ACHOQV_004575"
                     /pseudo
     CDS             5336..5549
                     /locus_tag="ACHOQV_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1898 a         1457 c         1710 g         1382 t
ORIGIN      
        1 cagcattgca atattcaccg ttaccgctgc cactgagcgc gaagcacgcg cccagctgcc
       61 gcacgcacat cttatttttg tcgcccgtat ccgtcaggag gtgactcatg cttaaaacct
      121 tccgcgtctt ttcccgtgct gttaacaagc gtggctatac cgttggcatc acgcagaacg
      181 tgaaggccga aaacgtccag gctgcaatca gcacagtaat taacaaatca gctaaagccg
      241 gtttatccgg cgtaattatc cacgcagtga catgtattca ggatgaggag taattcatgt
      301 gtaatttttc cgatatgact gttgatgaag cagcaaaaag cgcaatgcag gcagaactta
      361 tttgttctct gatgatgggt aacacaggtg aaatgactga gggtgagatt gagtcattgc
      421 tcgccctgat taagcagctt acaggtaagg ctggtagctg gctgattgcg gcatcgggtg
      481 atatgcaata agggaataaa taacaggatg aaataaatac cagcgaatta aagcgttaca
      541 tatcaggaaa taaaacgatg aaacgtaaat attctggctt aactgccagt agcaaaactc
      601 accccgaatt tatgtgcggg gatacttatc gcaatgaata cggtagccac atgacgatga
      661 tcagcagagg taagaaatga aaagcgcacc gaacttaaaa aaacagcctc gagggaaaaa
      721 acacgccgat acggaagtga ttattttcgc tggcagtgat gcgtgggcgc acgcgaaaca
      781 gtggctggag caggacggaa aacttgccgg agataatata ccgcccgtcg tgctggctga
      841 tgagcaactt aaagatattg gcaatctgca aattgtaccc gacgggcgca aatcagcgcg
      901 tatatacaag gccggacacc tcgatcaggt gatggtcaaa ggcataggtc agaagctggc
      961 ggcggcaggc gtacaggacg cagactatta ccccgaaggt atgcaccata gcgagcggca
     1021 aaactggcgt aactatctgg atattgaacg caaaaacatt tctgacgggc tggttattga
     1081 gttgcctgta aagaaaaaag cgcgtggaca gggtgatgat tcgtcactgg cacttaacca
     1141 gatgggggca agccagcgcg gagaagttct tctggcacat tatggcggcg aactggcaat
     1201 aaacgccgac tctgacaccg tgcattatta caacagtgtt gtatgggttc ccgtacagga
     1261 taaagagcta cagcgcacaa tggcgcaaat cttcattgac gaagatatct gctattcaca
     1321 aaacgccata aaatcggcgg ttgataccat gaagttaagt ttacccgtaa tggggaatac
     1381 cgcccgtaac ctgattggat tcagtaacgg ggtatttgat acccgtacag gtgatttccg
     1441 tgaacacgat aaaaatgact ggctgttaat tgccagtgaa ttaccgttca ccccaccagc
     1501 agagggtgaa acgctggcaa cacatgcgcc gaatttctgg aagtggttac gccgttcggt
     1561 ggctgagaat gaccgcaagg cagatcgcgt tctggcagcg ttatttatgg tgctggcgaa
     1621 ccggtacgac tggcagttat tcattgaggt aacaggtcct ggtggaagcg gtaaaagtgt
     1681 tatggcagag atttgtacca tgctggcagg taaggccaac acggtatcgg caagcatgaa
     1741 ggcactggag gatgcaaggg aacgcgcatt agtggttggc ttttcactga ttattctgcc
     1801 ggatatgacc cgctacgcgg gtgatggtgc agggataaag gccattacgg gcggtgacaa
     1861 ggtggcaatt gacccgaaac acaaagcgcc atactcaacg cgtattccgg cggttgtgct
     1921 ggcggttaac aacagcgcca tgtcattcag tgaccgcagc ggggggatct cacgtcgtcg
     1981 tgtgatattc aatttttcgg aggttgtacc ggaaaacgaa cgcgatccga tgctgccgga
     2041 aaaaatagaa ggtgagcttg ctgtagtgat tcgccatttg cttacgcgct tttctgacca
     2101 ggacgaagcc agactcctgt tacatgagca gcagaaatca gaggaagccc ttgcgataaa
     2161 gcgtgagggg gattcgctgg tggacttctg cggctatctc atgtcgtcgg taatgtgtga
     2221 cggcctgtta gtcggtaatg ctgagattat tcctttcagt ccacgccgtt atctctatca
     2281 cgcctatctg gcttatatga gggcgcacgg gttcagtaag cccgtaacac tgacgcgctt
     2341 tggtgcggat atgccaggtg caatggcgga atatggcagg gaatatatga aacggaaaac
     2401 gaagcacgga ttgcgctcaa atgtgacgct gacagaggaa tcggaggact ggatgccgtc
     2461 atgtgcacca gtgaaaaatg agggtgataa aaattaagtt atagaaaaac tgttcaccac
     2521 tgttcaccct gtaataaatg tatttagtat caatacgtta aggggtgaac agttttgtaa
     2581 aactattcac ccaagtgttc acctgttcac catgttgttt gatttttgaa cagcaaaggg
     2641 tgaacagggt gaacagttgg tgaacacctt tacaaaaagt gttcacccct taagttagtg
     2701 aaaataaagg caaaacagca aaaggtgaac agttgaacac cttttgggta taaattttta
     2761 tttattcctc tgagaaaaat tttatgacag agaaacacgc caaaaaatca cattcacacg
     2821 ctatcgattt aaccgaaccg gaatctgttg tgaggttcgt atcggtacga ggcagagcca
     2881 cgctttatat acctgacgaa catctgcatc actgcgacaa acataaactt ccgatcctga
     2941 ttgtctggaa gcgaacggta tacgccgatg tgacatggct gaatgattcg ctgatgttga
     3001 tacaccgtga cctgtttgaa cgtgaagagt tccggcggga tattgaagag cgggcaggaa
     3061 aaatctatga gaaatatgca gcaaacagca aaagagccgc acgggcgatc acttaccatt
     3121 tcatgacgct gtatgacctg aaagcggatg atgcagaaaa agcggcatgt gatttattcg
     3181 atatgacaat gggtattatc caggagtacc ggaacaagga gcgcagacca tgacagcaca
     3241 gattgcagct tacgggcggc tggtggacga cccgcaggta aaacagacca gcaagggaac
     3301 agcgatgacg ctggcgcgta tggcggtatc tttgccatgc agtcaggcac aggacggaca
     3361 ggcgacgtta tggctatcag tcctggcatt tggtaagcag gccgacgcgc tggcgagaca
     3421 tgaaaaaggc gaccttatca gcgtggcggg taacatgcag attagccagt ggaccggaca
     3481 gaacggagaa atacggcagg gctggcaggt tatcgctgac agcgtaatca gtgcccgttc
     3541 ggcgcgtcct ggcgggaagc gggggcaaca ggggaaggcg accgaagcac tggaaagagc
     3601 aaaacgacag accggacagc agggaaatca gccaccagcg ggaggtgatg acccgttctc
     3661 tgacgatatt ccgttctgaa tgacaggagc acaacatgaa ccagcagacc acacaacagg
     3721 accagaccac acgtaaacca tcacggcgca taccgtggca gcacatgaca aaagtgcaac
     3781 gaaaaaacca cagacgaata aatcgaattg cggaatactg gcctgagctg tttgggacag
     3841 agaatataaa acccctgaag gctggcataa tacgtgatgt gcttgaggac gctaaatcac
     3901 gggagttgcc acttgctaca ggtgtattac gcggcgcatt aacctcacac cttcacacga
     3961 cgcgatacct taaggcgatt attgctggtg gcccacggta tgacctgaac gggcagcctt
     4021 gcggcgaagt cacggaagaa gataaatcag tcgccagtga actactgaaa cggagactgg
     4081 cgcaaattaa gcagcagaac agccagcagc aaacaaccgg agacgatggt gatgatagct
     4141 gacagcgtgg cgcaaaaact tgaagaacgc ggattatggc gacgagcagc tacccgatgg
     4201 ggtgacgtcc ttcttcacgc agaaacggac agggaacgcg aggaagctgc cagacgtcgg
     4261 gcgatatgta tcattaaatc ccgtcgtatg cctgaacaac tggtcacctt tggcgatgtg
     4321 aaaaaggccg ctgacagaac gctgaaagag atggggatta atgcgcagga tgaatggaaa
     4381 aattacagct tcagtgatgc aggcgacgat ttagcgctac cgtgatacgt atctcatcac
     4441 cgtatttgct cagttatcgc gattcgctat aattaccacc gaagagccgt tgatggtgtt
     4501 cgcctctggc aactggagcc agactgcatg aagccattca gcggcttttc ggcactcatt
     4561 ttgatgtgta cttgtcacac acatcgggat gagtgaacga cgaaaacatc acggtgattt
     4621 cgtcatgccg cgataagaat gcgcctcttt gggcaactgg agccctctgt acagcgggtt
     4681 attgcggcaa agttgtgata aaaacgcgcc tctttgagca attgaagctc actgtaaagc
     4741 ggcttatcac agcaggctgt aacgggcgct catcttctgg caactgaagc cacctgtaca
     4801 gagacccgtt acggctgacc ctgaaggatt tcgccccgtg caattgaagc acccagcgga
     4861 gttattcagg gttttttatt gcccgtccac cagcacaacc agcaccctta ccagtacggt
     4921 taacctggtc attatcaggc aggccagaag ccccttcggt ggggtgaaaa atccacggaa
     4981 aaatccctga aaaaatgcac agaaaattgc gcaaaaaaat gcgcaaatcg atcacaaaac
     5041 cgatcacaaa accgatcaca cagaccagca gagcaaagcc agatatgacg aggcttgcag
     5101 ggggacagac cacctcaaaa agtgcgcaaa aaactgcgca tttttctcaa aaacgaaacg
     5161 caacagccgc gcaaaacgaa accgatacgt gatgatcgaa aaagtgcgca aatttaccgg
     5221 attcaggcaa ggctgtttac cggattcggg taagcaattt tgtctgattt gtgcaaaata
     5281 tggtgttgcc gtaatagtga cattgtcatc aaaacaggga cacacaggag ggccgatgaa
     5341 tgagcgttca ccaacagagg taattcacgg gattattgaa gaacaggagg agcgcgattt
     5401 aatcctcatg agtgaagagc agacagaacg ccagcgcaaa caggaggcca cacagccgga
     5461 cagcgtgacc tctgaaaatt atgcgcgtgt tgtggctgat gtgccgtgat ggtgtcgaac
     5521 tgagtagctt taagcgactc tgtgactagt tttaaacaag tcgcaaaaag agccggagaa
     5581 aaactacgaa tctggggttt ttgctaagca ctctgtacac ggtggaacat atggtggtca
     5641 ggagacggca ggagcaacgc cagaacgcgc ccccgtacca gtggcataag ccggagcggt
     5701 tacagcggta acttgttgat taaaaggcgg ctcaattctg agccgggtag ctgacggcgt
     5761 taactggctg atattgtacc aaagcacttt ttaatgcttt ggtgctgtaa cttactgaac
     5821 ttaaagcaaa gcgcaaaatt gcgctggcta caatgaaaaa cgtaatcgcc tgataattat
     5881 gccacgagac aaagggcggt gcaccctgaa tggtcataat gaccactcag accatcaagg
     5941 caactataac gaacatcgtt acagttggag cttgtacccc gttgggggaa aaggtgcata
     6001 cctgaatcat cattatggtg atcaagggta tgtaaagccc accagcctga ttaaaggtta
     6061 accggaaaaa gccaggtatg gagggagtgg ttagccacga acaagccgga caccaccagc
     6121 agaaggtatt atgattttcg taataccttt agcaaagcgt gaatcatcat taccgtaggg
     6181 atggtcataa tgaccacacc ttcccccact ggaacaagga aggctggctt acgaaaattt
     6241 cgtaaccctc cccagcacaa tgtattaaat aatcactaca ggccattgat taaacttaat
     6301 tatcgcgaat cgcgagaatt agccccgccg gaacaaggaa agggaaatta cgaaaatttc
     6361 gtaaaatccc cccacatctc ccacggttgc ccgttaagtg caagcgcaca ggcacgacct
     6421 cattaaccag ttaccagcgc tcacgct
//
LOCUS       DAZKGF010000224         6167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZKGF010000224 DAZKGF010000000
VERSION     DAZKGF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.224"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..302
                     /locus_tag="ACHOQV_004576"
     CDS             87..302
                     /locus_tag="ACHOQV_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9632842.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            476..733
                     /locus_tag="ACHOQV_004577"
     CDS             476..733
                     /locus_tag="ACHOQV_004577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632843.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMKTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            715..906
                     /locus_tag="ACHOQV_004578"
     CDS             715..906
                     /locus_tag="ACHOQV_004578"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9632844.1"
                     /translation="MCKRLLAIPRFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            1048..2067
                     /locus_tag="ACHOQV_004579"
     CDS             1048..2067
                     /locus_tag="ACHOQV_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9632845.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWGRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMREASAFKSGREFAAYVGLVPKQTGSGGKIRLLEISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            2788..3003
                     /locus_tag="ACHOQV_004580"
                     /pseudo
     CDS             2788..3003
                     /locus_tag="ACHOQV_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3178..5148
                     /locus_tag="ACHOQV_004581"
     CDS             3178..5148
                     /locus_tag="ACHOQV_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFP9632846.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYSPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGKLKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKSFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            5167..5958
                     /locus_tag="ACHOQV_004582"
     CDS             5167..5958
                     /locus_tag="ACHOQV_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFP9632847.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAANGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         1841 a         1372 c         1492 g         1462 t
ORIGIN      
        1 gatgagatgg tcttccccct ccttcacgac aacctttaat tacgtcatga tggataaact
       61 ttaaccacaa aagtaagagg gataccatgt ctcaacagaa aatcaccggc atcgatctgg
      121 ctaaaacgaa cttttatctt ttcagtatca atgctcatgg caaacccgca ggaaggatca
      181 aactttctcg caatcaactg cttaactggc tggtgcagca gcctaaaatg actgttgcga
      241 tggaggctcg cgggcatccc atcactgggc cagggaaatc aggaaactcg accatgatgt
      301 aatatttctt ccggcgcaac atgttaaagc ttatcagcga cgccagaaaa atgattatga
      361 cgacgctcag gcaatagctg aggcctgcca acatggaact atacgccccg taccaataaa
      421 aactctggag taacaggacg tccagacttt tttgaaaatg cgccggttgg tttcgatgga
      481 aaggacacaa ctgataaatc acgttcgtgg acttcttgct gaatatggca ttgtgttcag
      541 taaaggcgca acagaattac gccaaaaatt accggcatta cttgaggatg cggaaaacga
      601 actcactgat accatgaaga cgttattgca tcggcagtat atacggttga ttacactaga
      661 taatgaactg gagtggtatg attctgaact gaaaaatatg tccgccaaga tcctatgtgc
      721 aaacgcttgt tagccattcc tagattcagc cctctggtta gtcaggcaat aaaaagctgg
      781 atgggggatg gaaaacagtt tagacgagga agagatgcat ctgcgactct gggactggtt
      841 ccccgacagt tcagcacagg tgggaagcag gttcttctgg gtataacaaa atttggagaa
      901 atgtaatgaa cacatcccac cccattaagt caggtgctct gtgatggatg aagggaaata
      961 ctggaaggtg tggagcctcg cagatttccg gcatcatagt gcccaataca ggatgcataa
     1021 agctccctca gactggagac tccacaaatg aaatatacac cggttggcgt tgatatcgca
     1081 aaacatctga ttcaggttca tttcatcgac gagcacacag gtgaagtgat tgataagcaa
     1141 ctacgtcgtc aggattttct gatgttcttc tgtaatcggg aaccctgcct gattggtatg
     1201 gaggcctgta gaggctctca gcactggggg cgtgaactga caaagctggg gcataaagtc
     1261 cggttgttac aggcccactt tgttaaggca ttcgtcatgg gaaataagaa tgatgtgatg
     1321 gatgcccggg ctatctggat ggctgttcag cagccaggta aggacatcgc ggttaaaaca
     1381 gaagaacaac agtcagtgct ggttttgcac cgttcccgca gacaattggt taagttccgc
     1441 accatgcaga ttaattctct gcacggcacg ctcctagaat ttggtaaaac cattcacaaa
     1501 ggtcgggcag cgatggagcg agagctccct gaagcactgg agcggatgaa agagagactg
     1561 ccgccgtatc tcattactgt gctggaaaac cagtacaacc ggctgaatga gctggactcg
     1621 ctgatagagg atattgaaaa aaagcttacc agcgtggcga gacagaatga aacctgtaag
     1681 cggttgctgg atattcccgg cgttgggcca cttattgcaa cggcagcggt ggccaccatg
     1741 agggaagcat cagcgtttaa atcagggcga gagttcgcgg cttatgttgg tctggtgccc
     1801 aaacagactg gctcaggagg gaaaatccgc ctgctggaga taagcaaacg tggtaataca
     1861 tatctgcgag ccttgtttat tcatagcgca agggcggcag cattactgcc caaagaacct
     1921 ggtccgtgga taaccgaact gaaaaaacgt cgtccaacca gcgtggcaat cgtcgccatg
     1981 gcaaacaaac tagcacgaac agtatgggcg atagcagctc atggaagtaa atacgacaag
     2041 aatcatgtca gtatcagatc atattaatcg ctgataccga taaatattaa cttttaatga
     2101 aaggatgaat actgaaaggt aactctggcg gccatagtga tgacaacgac aggtaagacc
     2161 gggactcact aaacctgaat attgtgttga gctaaaagct cgctggagaa atgaggtgtg
     2221 agtcagcgaa ttacataggg gctcgcagct tcatggctgc aaaaaagccg gatataaagc
     2281 tgcaacctac ccgtcactgt caaggcaatg gatgccttgc aacgggatgt gttcatataa
     2341 tcaatatgtc agatctatgc tgatacaggg tgccagagcg gtattgttca gagcctcaga
     2401 gaaaactgat tcacttcgtg tgtgggttaa ttgtattcgg gaaaagcgtg gcttcaacca
     2461 ggcagttgtt gctcttgcaa ataaactcct tcggatcgcc tgggcaatta tcgcccgcaa
     2521 tgagatgtat ttgcccgcag caaaacaaaa tcaataatct acgtaaaatc agtaggagta
     2581 aagcttaccc tgaaattgca aagaatgcag aatgatgaaa aacaggtcag accgacgtta
     2641 cgaaaacctg caagatgagc caggccagta aggccgagta agcgataagg tcggagcgcg
     2701 cgaatgtctc atcaaggcca gaagcaataa cttcatgaac aaaccggata tacgaaagta
     2761 aacctgtcct tatcatagtt gctgtactct tgcaatacgg gggaagacca tctcatcgct
     2821 tacgaacaat tcgtgaaaac gatgaagaaa gactacatcg cgtgcatgcc gaaatctaat
     2881 gtaagaacgg cattgcataa tcttgcagtg gagatcgggt attacaatga aaaccatccg
     2941 catagtgcgt taggttatct cccaccaagg gaatatcaac gtcagcgaat aacgtcacct
     3001 tagatgcagt agttgtcagg aaatagtgac tcaagggcag gcttaccata tacactctta
     3061 gtggttcgac tgggtattag aatagctatt aacagattag cactgaacac tgcgtaatag
     3121 tggttgcaca aacaaaatgt tatgttataa cattgctgta ttgacatata ttttcgaatg
     3181 tatatgaacg taatcagaac tgtcatttgt acattaatca tacttccggt ggggttacag
     3241 gcagcgacaa gtcattcttc tatggctaaa gatacaatca ccgttgtcgc gacaggaaat
     3301 cagaacacgg tatttgaaac gccgtcgatg gtcagtgtca tcacgaatga cacgccatgg
     3361 agtcagaatg cggtcacatc agccggtatg ctgaaaggtg ttgcgggtct cagccagact
     3421 ggtgcaggac ggaccaatgg gcagaccttt aatctacgcg gctatgacaa aagcggggta
     3481 cttgttcttg ttgacggcgt ccgtcaactc agtgacatgg caaaaagcag tggcacttat
     3541 ctggatccgg cactcatcaa acgcatcgaa gttgtccgcg gaccaaactc cagtctgtac
     3601 ggcagtggcg ggctgggggg ggtggtggac ttcagaactg ccgatgctgc agattttctt
     3661 ccccccggag agacaaatgg tgtaagtctg tggggaaata tcgccagtgg tgaccacagc
     3721 acaggctcgg ggctcacctg gttcggtaaa accggaaaaa cagatgcgct tctttctgtc
     3781 attatgcgta aaagaggaaa tatctatcag agtgatggtg agcgtgcacc taacaaggaa
     3841 aaacctgcag ccctgtttgc gaaaggctct gtcagtataa cagacagtaa caaagcaggt
     3901 gccagcttgc gtctctacca gaataacacc actgaaccgg gcaatcccac tctgacacat
     3961 ggtgacaacg gcctgcgtga cagaaaaaca gcacaaaatg acgtgcagtt ctggtaccag
     4021 tactcccctg cggataacag cctcatcaat gtaaagtcaa cgttatatct cagtgatatc
     4081 actatcaaga caaacggtca caacaaaaca gcagaatggc gaaacaacag aacctccggt
     4141 gttaatgttg tcaacaggag ccatactctg atttttccgg gagcccatca gttaagttat
     4201 ggcgccgaat attaccgtca gcagcagaag ccggaaggct ctgccacact atatccggaa
     4261 ggaaacattg actttacatc gttgtatttc caggatgaaa tgacaatgaa aagcaacccg
     4321 gttaacatta tcgtcggttc ccgttatgac cggtacaaga gcttcaatcc ccgagccgga
     4381 aaactgaaag ccgaacgcct gtcccccagg gcggcgattt cagtctcacc aacagactgg
     4441 ctgatgatgt acggctccat atcctctgca ttccgtgccc ccacaatggc agaaatgtac
     4501 agggatgatg tacattttta ccgcaagggt aaacccaatt actgggtacc taaccttaat
     4561 ctgaaaccag aaaataacat tacccgtgag attggcgcag gtattcaact ggacggcctg
     4621 cttacagaca atgaccggct gcagttgaaa ggcggatatt tcggaacgga tgccagaaac
     4681 tatattgcca cgcgcgtgga tatgaaacgg atgcgttctt attcttataa tgtatcccgg
     4741 gcccgtatct ggggatggga tatgcagggt aattaccagt ctgattattt tgactggatg
     4801 ctttcttata accgcacgga aagtatggat gccagtagca gggaatggct gggctccggc
     4861 aatcctgaca cactcatcag tgacatcagt atacctgttg gtcatggagg agtttctgcc
     4921 ggatggcgtg ctgaactttc ggcagcagcc acgcatgtga aaaaaggcga tccccatcag
     4981 gctggctatg ccgtacattc cttttcactg tcttataagc ctgtaagtgt taaaagcttt
     5041 gaggcatcag tgactctgga taatgccttc aacaagcttg ccatgaatgg caaaggtgtg
     5101 ccgctttcag gcagaactgt cagtctttat acccgttatc agtggtaacc tcgcaggaaa
     5161 taaatcatga tgaaaaatac aggctatatc ttagctcttt gtctgacagc atcggagcat
     5221 gccctggccc acgatgtctg gattaccggt aaacaggcag agaacaacgt taccgcagag
     5281 attggttatg gtcataattt cccctcaaag ggcacaattc ctgacagaag gaatttcttt
     5341 gaaaatcccc ggctttataa cgggaaagag ataataacgc tgaagccaac gtccacggat
     5401 tatgtctata aaactgagtc tgcagccaac ggtaatagct acgttctgtc aacgaaaatg
     5461 aaaccgggat actggtcgag aacctcgtca ggatggaagc ctgtcagtcg ggagggcaga
     5521 aatgatgtgg catactgtga gtttgtgact aaatatgcaa aatcttttat tcctggtgaa
     5581 cagcagatgc cagcacaact ctatcagtct ccaacagggg atgagcttga aatcattcca
     5641 ttatccgata tcagtcgttt cggtgaagat gtgaagctga aagttctgta taaaacatcc
     5701 ccgcttgccg gagctactct ggagcttgac tctgtcagtt atctgaaatc atcccgtcat
     5761 acccatgcag ctgagcataa acattcggct cataaagcag aacttacctt tgtaagtaat
     5821 gaggacggta tcatcactgt accttccctt catgtcgggc aatggctggc taaagtcaaa
     5881 aataagaaag tatttccgga caagagtctg tgtgatgaaa ctgtcgatgt ggcaacctta
     5941 agcttctccc gaaattaata atactattcc tgccctgatt tatgggcagg gtattatcaa
     6001 tacgagaata gcctaccgac atccgtgtaa aaaaccacat aatgattttt catatccatt
     6061 tccagatata cttatgtaaa gatcacctcc aaaaaagcgt aatagtaata aaaatccctg
     6121 aagcaccctg ttcaaaccag aaggaataca taacaagaca ataaaat
//
LOCUS       DAZKGF010000225         6151 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZKGF010000225 DAZKGF010000000
VERSION     DAZKGF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.225"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            225..2072
                     /gene="ldtD"
                     /locus_tag="ACHOQV_004583"
     CDS             225..2072
                     /gene="ldtD"
                     /locus_tag="ACHOQV_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9632848.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVVGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2253..2801
                     /locus_tag="ACHOQV_004584"
     CDS             2253..2801
                     /locus_tag="ACHOQV_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFP9632849.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2828..3475
                     /gene="gloC"
                     /locus_tag="ACHOQV_004585"
     CDS             2828..3475
                     /gene="gloC"
                     /locus_tag="ACHOQV_004585"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFP9632850.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVSVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVALQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3526..4716)
                     /gene="aspC"
                     /locus_tag="ACHOQV_004586"
     CDS             complement(3526..4716)
                     /gene="aspC"
                     /locus_tag="ACHOQV_004586"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFP9632851.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETIDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(4901..5989)
                     /gene="ompF"
                     /locus_tag="ACHOQV_004587"
     CDS             complement(4901..5989)
                     /gene="ompF"
                     /locus_tag="ACHOQV_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFP9632852.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1539 a         1516 c         1568 g         1528 t
ORIGIN      
        1 gaaaactccg tttctgcact aaaaaagtgg cacattacgg cgcgcttttc tttaaacttc
       61 tttacattag gttatgtaaa aacgtatcgg cgtttatata ctgaagataa gcctgatgag
      121 taacaggctt gctcgtcata ctttcgtgag tattggcgtt gtacaggcaa gtcgtaaaat
      181 aacagcctgg ctattcagag tatgataaaa acagggggca agggatgttg cttaatatga
      241 tgtgtggtcg tcggctgtcg gcaatcagtt tgtgcctggc cgtaacattc gctccactgt
      301 tcaatgcgca ggccgatgag cctgaagtaa tccctggcga cagcccggtg gctgtcagtg
      361 aacagggcga ggcactgccg caggcgcaag ccacggcaat aatggcgggg atccagccat
      421 tgcctgaagg tgcggctgaa aaagcccgca cgcaaatcga atctcaatta cccgcaggtt
      481 ataagccggt ttatcttaac cagcttcaat tgttgtatgc cgcacgcgat atgcaaccca
      541 tgtgggaaaa ccgtgatgct gttaaagcct tccagcaaca gctggcagag gtggcgattg
      601 ccggtttcca gccgcagttt aataaatggg tagagttact gaccgatcct ggtgttaacg
      661 ggatggcacg cgacgtggtg ctctctgatg cgatgatggg ctatctccat ttcattgcaa
      721 atattccggt caaaggcact cgctggctat atagcagtaa accttatgcg ctttcaacgc
      781 cgccgctttc ggtgattaac caatggcagc tggcgctgga taaaggtcaa ttgcctacgt
      841 ttgttgtagg attggcaccg cagcatccgc aatatgcggc gatgcatgaa tcgttactgg
      901 ccttactcag tgacaccaaa ccgtggcccc aactgaccgg caaagcaacg ttgcgcccag
      961 gacagtggag taacgacgta ccggcgttgc gcgaaatatt gcaacgtaca ggcatgttgg
     1021 acgggggacc gaaaattacg ctacctggcg atgacacgcc aactgacgcg gtagtcagcc
     1081 catccgctgt tactattgaa acagcagaaa ctaagccgat ggacaagcaa actacgtctc
     1141 gtagtaaacc tgcgcctgtc gttcgtgccg cctacgataa tgaactggtg gaagccgtta
     1201 aacgttttca ggcatggcaa ggattggggg cagatggtgc tattggcccg gcaacgcgtg
     1261 actggttaaa cgtaacgccc gcccagcgtg ctggcgtgtt ggcactcaac atccagcgat
     1321 tgcgcttgct gccaacagag ctttctaccg ggatcatggt taacattcca gcctattcgc
     1381 tggtctacta tcagaacggc aatcaggtgc tggattcgcg agtcattgtc ggtcgccccg
     1441 atcgcaaaac gccgatgatg agcagtgcac ttaacaacgt agtggtaaac ccgccgtgga
     1501 acgtaccacc aactctggca cgcaaagata ttctgccgaa agtgcgcaac gatccgggat
     1561 atctcgaaag ccatggctat acggtgatgc gcggctggaa cagcagagaa gcgattgacc
     1621 catggcaggt tgactggtct acaatcacgg cctcgaattt accgttccgc ttccagcagg
     1681 ctccaggccc acggaactcg ctggggcgct ataaattcaa tatgccgagt tcagaggcca
     1741 tttatttgca tgacacgccg aaccacaatc tgttcaagcg tgatacacgc gcattgagct
     1801 caggctgtgt acgagtgaat aaagcttccg atctggcgaa tatgctgttg caggatgcag
     1861 gctggaatga caaacgtatt tctgatgcgc tgaagcaggg cgatacgcgt tacgtcaata
     1921 ttcggcagtc gattccggtg aatctctact atctgacggc ctttgttggt gcagatggtc
     1981 gtacccagta tcgtacagat atttacaatt atgatctgcc tgcgcgatcc agctcgcaaa
     2041 ttgtatcgaa agcggaacaa ttaatcaggt aaatgaagca gttcggggaa atcagttgtc
     2101 gtaataatta gcatgattgg gggcaattct ctgcagcccc cgtcactgct ggggttgagt
     2161 catcttgacg tctgctttac gggcggttaa ggtgcctctt gtgcgccaga agtgcatata
     2221 aacgataaca ttgacctgta gacttgatta tcatggacaa attcgacgct aatcgccgca
     2281 aattgctggc gcttggtggc gttgcactcg gtgccgccat cctgccgacc cctgcgtttg
     2341 caacactctc taccccacgc ccgcgcattt tgacactcaa taatcttcat accggagagt
     2401 caatcaaagc ggagtttttc gatggcagag gctatattca ggaagaattg gcaaaactta
     2461 accatttttt ccgcgattac cgcgcgaaca aaataaagtc catcgaccca ggattattcg
     2521 accagttgta tcgcctgcaa gggttgttag gtacccgcaa accggtgcaa ctcatttccg
     2581 gttatcgttc cattgatacc aacaatgaac tacgcgcccg cagccgtgga gtagcgaaga
     2641 aaagctatca cacgaaaggg caggcgatgg atttccatat tgaaggtatc gcgttaagca
     2701 atattcgcaa agccgcgtta tctatgcgcg caggtggtgt aggatattac ccacgtagta
     2761 actttgtgca tattgatacc gggccagcac ggcactggta gcaattgctt aacgaaacag
     2821 gggcagtatg aactatcgta ttattccggt caccgcattc tcccagaact gttcattgat
     2881 ctggtgtgaa caaacccgcc tggccgcact ggtcgatcct ggcggcgatg cggaaaaaat
     2941 caaacaggaa gttgacgcca gcggcctgac actgatgcag atcctgctga cgcatggtca
     3001 tctggaccac gttggtgcag cggcggaact ggcgcaacat tacggcgtgt cggtttttgg
     3061 cccggaaaaa gaagatgagt tctggctgca aggcttgcct gcgcaaagtc gtatgtttgg
     3121 tctggaagag tgccagccgc tgacgccgga tcgttggctg aacgaaggcg ataccatcag
     3181 catagggaat gtggctttac aggtgttaca ttgccctggg catacgccgg gtcatgtcgt
     3241 gttttttgat gatcgggcaa agctgctgat ttctggcgac gttattttca aaggcggagt
     3301 agggcgcagt gacttcccgc gtggcgatca taatcaactg atttcttcaa tcaaagataa
     3361 gttgctgcca ctgggggatg acgtgacatt tattccgggt cacggaccat tatccacact
     3421 tggttatgag cgcctgcata atcccttcct gcaagacgaa atgcccgtct ggtaaaggcg
     3481 cataaaaaag cccgcttttt aagcgggctt cattgttttt aacgcttaca gcactgccac
     3541 aatcgcttcg cacagcggag ccatattatc tggtgtcatc ccggccacgt ttacgcgacc
     3601 agaagcaaca gcatatacgc caaactcttc gcgcagacgc agcacttgtt cttttgtcag
     3661 gccactgaag gagaacatgc cgttctgttt gatgataaag ctgaagtcgc gatttgcgcc
     3721 tttttcctgc agcgtattga cgaacaactg acgcatacgc tgaatacgct ggcgcatatc
     3781 agtcagttct tgttcccaaa tcgcacgtaa cgcatcgttg ctcaggatgg tggcaacaac
     3841 agaagcgccg tgtgctggtg ggttagagta gttagcgcga atcgccgctt tcatttggct
     3901 gaatgcgcga tcaatggttt cactgtcagc agcaaccaga gtacaagcgc caacacgctc
     3961 gttgtacagg ccaaagtttt tagagtagga actggcaaca atcagctctt tatgcatagc
     4021 cgcgaaagcg cgcagtcctt cagcatcttc ttccagacca cgggcaaacc cctggtaagc
     4081 gaagtcaaac agcggtaacc agcctttctc aacggaaagt tgtgccagtg tctgccattg
     4141 ttccagcgta gggtcgatac cggttgggtt atggcagcag ccatggaaca gcaccacgtc
     4201 gccagcctga gcttcgttca ggctgttaat cagtgcatcg aagtcaaggg tgtgattttc
     4261 cgcatcataa taagcgtatt cacgaacttc cagacctgca gagttaaaga cgctcttatg
     4321 gttcggccag cttgggttgc tcacccacac acgcttaacg ctggtatttt ttgccaggaa
     4381 atcggcagcc acgcgtagtg cgccagtgcc acccggagtc tgtgccgtgc gagcacgttt
     4441 gtcattgatc agggcgctac ctttaccaaa cagcagttcc tgagtgcagc gaccaaattc
     4501 agggatgccg tcaatgccga ggtaattttt ggtggtttca ttttcgagca gatactgttc
     4561 agcctttttc acactggtca gtaccggggt tttgcccgtc tcatctttat agacaccaat
     4621 cccgaggtta attttgccgg gacgttcatc ggcacgaaac agatcggcca ggcccagaat
     4681 cgggtcggca ggagcggcgg taatgttctc aaacatgacg aggttccatt atggttacag
     4741 aagggaagtc cgctatcagg gtaacgggag atttacaaaa ttccaactat tactgatgaa
     4801 aacgcaggct gtttttgcaa gacgtgagat tgctctggaa ggcataaaaa aaacaggacc
     4861 aaagtcctgt tttttcggca tttaacaaag aggtgtgcta ttagaactgg taaacgatac
     4921 ccacagcaac ggtgtcgtca gaacctacgc ccagtttgtt gtcagaatcg atctggttga
     4981 tgatgtagtc aacataggtg gacatgtttt tgttgaagta gtaggttgcg cccacttcaa
     5041 agtagttaac cagatcaaca tcaccgatac cttctacgtc tttcgcttta gatttggtgt
     5101 aagcgatgga cggacgcaga ccgaaatcga actggtattg cgcaactaac agaacgtctt
     5161 gcgttttgtt ggcgaagccg ctgatgtttg taaatttatt agtgatcggc gtagcgttac
     5221 gggtttcacc gtagttcgct gccaggtaga tgttgttcgc gtcatacttc agaccagtag
     5281 cccactgttc agctttttta ccgttgccaa gaggttgagc ttcttgcagg ttggtacggt
     5341 cagcggcgcc ataagcacca acgataccaa agccttcgta ttcgtagctg atagaaccgc
     5401 caacaccgtc gccgttagag cggcgtgcag tgtcacgctc gtttttaccc aggtactgaa
     5461 cagcgaagtt caggccatca accagaccaa agaagttgga gttacgatag gtagcaacgc
     5521 cgccaacacg accaacgaag aagtcatcgc tgtatgccgt gtcaccgcca aactcaggca
     5581 gcatatcggt gtaacccagt gcatcataaa ccacaccgta gttacggccg taatcgaaag
     5641 aaccaacgtc agcgtattta agacccgcga atgccagacg cgttttgtta ccagtttgag
     5701 cgtcagcgcc ttcagagttg ttaccctgga agttatattc ccactgacca taaccggtca
     5761 gatcggaatt gatttgagtt tcccctttaa aaccaagacg ggcataggtc atgtcaccat
     5821 tgccaccgta actgttttca ccgttaccct tggagaaata atgcagaccg acagctttac
     5881 cgtacagatc tactttgttg ccatctttgt tatagatttc tgcagcgttt gcagtacctg
     5941 ctactaacag agcagggacg atcactgcca gaatattgcg cttcatcatt atttattacc
     6001 ctcatggttt ttttatgaca cctgccactg ccgtcaataa gttctgtcaa taaaaattta
     6061 cggaactatt gatgagagtt tggtgtcttt atgtgtctgc aggcatcttt ccattcaaac
     6121 taacgtttcg ctaccgtgaa agtgctacaa a
//
LOCUS       DAZKGF010000226         6147 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZKGF010000226 DAZKGF010000000
VERSION     DAZKGF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.226"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..821
                     /locus_tag="ACHOQV_004588"
     CDS             <1..821
                     /locus_tag="ACHOQV_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9632853.1"
                     /translation="TVAVLVPSLTDKTSSRFMQSLQQVLNKNEFQLLLGCHEYNQLKE
                     AEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPFSPAQITIETAFFE
                     ASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSYYQNSDLVITMPGAAS
                     LQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPGNIAVACLDGSDSC
                     DQTHPTLTSIRIDYKKMGTETGKLLIGLLNNNHDESEESRIVQFNYQIELRQST"
     gene            1023..1703
                     /locus_tag="ACHOQV_004589"
                     /pseudo
     CDS             1023..1703
                     /locus_tag="ACHOQV_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1720..2028
                     /locus_tag="ACHOQV_004590"
                     /pseudo
     CDS             1720..2028
                     /locus_tag="ACHOQV_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2131..3237)
                     /locus_tag="ACHOQV_004591"
                     /pseudo
     CDS             complement(2131..3237)
                     /locus_tag="ACHOQV_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            4003..6051
                     /gene="ireA"
                     /locus_tag="ACHOQV_004592"
     CDS             4003..6051
                     /gene="ireA"
                     /locus_tag="ACHOQV_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFP9632854.1"
                     /translation="MKNKYIIAPGIAVICSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKEDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWRVSANYTFTDSRRESDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGMNYQFLPGTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRISF
                     "
BASE COUNT         1742 a         1358 c         1423 g         1624 t
ORIGIN      
        1 atacagtcgc cgtactggtt ccttccctga cagataaaac cagctcccgc tttatgcagt
       61 cgttgcagca ggtgcttaat aaaaatgaat tccagctact gcttgggtgt catgagtata
      121 atcagctcaa agaagctgaa atcctgatga cgcttcttca aggtaacccg gcggcgttag
      181 ttatttttgg ttctcagttg gctgataaaa ctcaccagat actcgagaaa acaaatatac
      241 ctaccattaa cgttgtcggt tcccctttca gtccggctca aattaccatc gaaacagcat
      301 tttttgaagc atcacataaa ctgaccgaac atctgctgga acagggttat aagaatatcg
      361 gatttatcgg tgcgcatatg gacaaccgcc tgcaacgtca gcaactgaac ggctggcata
      421 aagcaatgct gagctattac cagaactctg atcttgttat caccatgccg ggtgccgcaa
      481 gtttgcaatt ggggcgttac gcattaaacg agatattact gcgacaacca gagctggatg
      541 ctgtgatttg cagccacgaa gagattgcgc tgggtatcat gtttgagtgc cagcggcgtt
      601 tgttaaaaat ccctggaaat atcgctgtag cttgtcttga cggttcagac agttgcgatc
      661 aaacacaccc aaccttgaca tccatccgta ttgactacaa aaaaatgggg acagagacgg
      721 gtaagctgct gattggtcta ttgaataata atcatgatga atcagaagag tcgcgtatcg
      781 tgcaattcaa ctaccagata gaattacgtc agagtacatg agctcaaaag gggattattg
      841 caaagttagc tattgtggta gggatagccg tttccgctat tccccgcgca gagccggatg
      901 tgtgctgcta acgcatcctg tcaacgacgg atgaaaagtt atccacttat atctccacca
      961 acggcccaat attgtatcgt tcgtgacaca tccatttcta cttcaggtat ttggcgtagt
     1021 agcgggtgct aaaaatttgc cgcaaaaccg caatatcagc ccgcagaggc gctctccaga
     1081 ttatccattc ctatattatc tctgcaccgg atgaattact cgaacaactc agaaatatga
     1141 cacatatgca acttatcagg actctgggat cctggaggcc tgatgccagt gaatacagta
     1201 atgttaccaa cgtttatcgc attacattga aatcccttgt tcgacgctat ttcgagtttc
     1261 atgatgaaat caccaacttg gatgtcatga ttgcggcaat tgttgatgaa ctggcacctg
     1321 aactgattaa acgtaatgct gttggatacg aaagcgcttt gcagttgttg atctctgccg
     1381 gagacaatcc ctaacggtta agaaccgaat caggttttgc ggcgttgcgc ggtgtcagcc
     1441 agtttcccct atcttctgga aaaacgaacc attaccgact taatcggggt ggggagcgtg
     1501 ttgcaaatag tgcactttac atcatcacta taggatgttt acaaactgac gataaaacaa
     1561 aggaatatgt ctccaggcga gtagccgaag gacatacaaa aatggaagca atacgctgtc
     1621 tgaagcgcta tatctcacgc gaagtttata cattactacg aaatcaaaac aggcatgtta
     1681 actgcatccc gataacgtct tgactcttag aagggcgtcc aaggcagtca ttattcgagc
     1741 aggcagttcc ggcagttatt gtgtcgatac cagatcaggc agagtatgag ccggcgcgga
     1801 aaatgctggg ataacagctc aatggaacgc ttcttcagga gtctgaagaa cgaatggatg
     1861 ccggtggtgg gttacgtaag cttcagcgag gcagctcacg ccataacgga cgatatcgtt
     1921 ggatattaca gcgcactaag accgcacgaa tataacagtg ggttatcccc aaacgaatcg
     1981 gaaaatcgat actggaaaaa ctctaactcg gtggccagtt tttgttgacc acttcagcca
     2041 tcgccagacg ctaacatgag ttactcttgt tctgggctac gtcaccttca gggaactggt
     2101 tatcctcatt ctttatttgt gacattgtgg ttattgtgcg acaaacatgg ctatcccgcc
     2161 ccatatccca gataaaacca cctgctcacg tgcgacagcg acaattgtaa tattgacatt
     2221 ctttcctttg gcctgaagtt ttcgagacct cttgcaaagc ctgagttgag catcccatgc
     2281 tctgtcaatg acggggcggg gtaaattttc ctgatttttt gtattgccgg actgatgcgt
     2341 gtcggatgcc ggtaactcca cgctgcctca accaatagct ttctggcata gctgtttccg
     2401 gctttggtaa tgctgccctg atgtctgctt ccacccgatg aatattctga tggcgtgata
     2461 ccaaaccatg acatcagttg tcgtggatgt tcaaaacggg tcagatcacc cagttcagaa
     2521 attaatccaa ccgcagtaat aaactgaatc cctcgcatgg cttgctgcgc ctctactacc
     2581 ggatacagcc tccattcggt aacggcctcc ttcgggcgga ttccagccgt tcacattgtg
     2641 cctgccggtc ttctattgta caacggtgtt cgtcaaaagc taattgtcgc cagggacttt
     2701 cgaatgagta tttgctgagc cagtgtcggt gtgcagggcc ccaatctgct cgcccgacat
     2761 aatggacccc atgaaccaga agaaacgatt tcagacgttg ccttgcatgc cgtaaatcat
     2821 cgcgggcaga tgcccatgcc cgggccaaat cccggaatgt ctcatcttca acgccggata
     2881 cgtagacagc agaaagatct cctgcacgca gtgaacgcac aagtctgatg gcatcacggt
     2941 gtcggtttta ccccgctccc ccggtttttt cgggataagt gagggggcgc agaccatgca
     3001 gtcaaaaccg gatttcacca gccgacgata aagtccataa ccacagggtc ccgctttata
     3061 aacgatactg acctgcgatg actttgacct gatacgttga taaagattat gaatgtcagt
     3121 aggtgatgtg ctaattttac ccatcagttc aacaggttct gaattgatag cataggagac
     3181 agtaatcgat tctttgtgaa catctaattc gacgtacaac atgttattgt tgttcatgct
     3241 ggcctccgtg gtggaaattc caggggggta gcaacagctc acccatgcgg ctctgacaac
     3301 gatgttaacc cgcattaaat atcccacgtt ggacagcatc tgtctcacgg aaagtcatac
     3361 tgccttacgt gcatatttca ccttacgcct tcactgtctg tcatcggaag tactgattta
     3421 tattggggct ttgcacttac tgtcattcac gggaaaagat ggtggtgaac agtacgatac
     3481 tgtcttggct gttgccgagg gaagattcag gagtggtaag ggggttagtg gtgtaagatg
     3541 agtctggagc tctgactggt cagtgacgcg aaactcaatc tgccagggta aactccccct
     3601 tgcttttgcg cagaagcagt gggccttggt tcgcttatgt cctgcatcta caagcgaatg
     3661 actgaccgga gactcccata tctggtcagt cgcccatttt tattttccac cacatcggac
     3721 aagcataccg taataaacag caccagtctc ctgttcactc ttgcttgatt tactggaatg
     3781 ctgtttatgg agatatccgg ttgaaagatt ttacctcttc gacaggacta taaattatac
     3841 agtaagaagt ccctcgtaga aaggggctga ttctcatcaa gtcggacagg ttcaaagtgt
     3901 tacctgaatg agctaaatgt tatttacctt cctttctgtt cattgtaaat ttttcttgct
     3961 aacgcaaatc attatcatta cgattttaga aaagggaaat ttatgaagaa caaatatatc
     4021 attgctccgg gcattgccgt gatatgttct gcagtcatat catcaggtta tgccagttct
     4081 gataaaaaag aagatacgct tgttgttact gcctccggct tcactcagca actcagaaat
     4141 gcaccggcca gtgtttcagt cattacttca gagcaactgc aaaaaaaacc ggtttcagac
     4201 ctggtcgatg cggtaaaaga tgttgaaggg attagtatca ccggtgggaa tgaaaaaccg
     4261 gatatcagta tacgtgggtt aagtggcgat tatacattga ttctggtcga tggacgacgt
     4321 cagagtggtc gggaatccag accaaacggc agcggcggtt ttgaagccgg atttatccct
     4381 cctgtggaag caattgagcg tattgaagtt atacgtggcc ctatgtcttc cctgtatggt
     4441 tccgatgcta tcggaggagt tattaatatc ataaccaaac ctgttaataa ccagacatgg
     4501 gatggcgtac ttgggcttgg gggaattatt caggaacatg ggaaatttgg taactcaacc
     4561 acgaatgact tctacctgtc aggcccattg gttaaggata aacttggtct tcagttatat
     4621 ggaggaatga actatcgcaa ggaagatagt atctctcagg gaacaccagc aaaagataat
     4681 aagaatataa cggcaacact ccagtttact ccgactgaaa gccagaagtt tgtttttgaa
     4741 tacggaaaaa ataaccagga acatacatta acacctggtg attctcttga tgcctggact
     4801 atgcggggaa atcttaaaca accaaacagt aaaagagaaa cacataactc acgtagtcac
     4861 tgggtagcag catggaatgc ccagggcgaa atactgcatc ctgaaattgc tgtttatcag
     4921 gagaaagtta ttcgtgaggt taaatctggt aaagaagata aatataatca ttgggatctt
     4981 aattacgagt caagaaaacc ggaaataacc aacacgatca tagatgcaaa agtgacggca
     5041 tttctgccgg aaaatgtgct gaccatcgga ggtcaatttc agcatgcaga actccgtgat
     5101 gactcagcca caggtaaaaa aacgacagaa acacagtctg tttcagttaa acagaaagct
     5161 gtttttatag aaaatgaata tgctgcaacg gattctctcg ccctgactgg agggctgcgc
     5221 ctcgataacc atgaaatcta tggcagttac tggaatccaa gattgtatgc tgtttataac
     5281 ctgaccgata atctcactct caaaggaggg atcgcaaaag catttcgggc cccttcgatt
     5341 cgtgaggtca gtcctggatt tggaacactg acgcagggtg gtgcctctat tatgtatgga
     5401 aacagggacc tgaaaccgga gaccagtgta accgaagaga tcggtattat ttataataat
     5461 gacagtggtt tttcagcaag cgcgactctg tttaatacag attttaaaaa taagctgacc
     5521 agttacgata taggcacaaa agatccagtc actgggctaa acacttttat ttatgataat
     5581 gtgggtgagg caaatatcag aggtgtggag cttgcaactc agattcctgt gtatgataaa
     5641 tggcgtgtat ctgcaaacta tacattcact gactcccgtc gagaaagcga tgacgaaagt
     5701 ctcaatggca agtcgctgaa aggggaacct ctggaaagaa ctcccagaca tgcagccaat
     5761 gcaaaactgg aatgggatta cactcaggat attacatttt attcatctct gaattataca
     5821 ggaaaacaaa tctgggcagc acaaagaaat ggtgccaagg ttccccgtgt tcgtaatgga
     5881 ttcacttcta tggatattgg tatgaattac cagttcctgc caggcacgct gattaatttt
     5941 gccgtactta acgtcacaga cagaaagagc gaagatatcg ataccattga tggtaactgg
     6001 caggtcgatg aaggacgtcg ttactgggct aatgtaagaa tatccttctg agagaatata
     6061 gataacctga ggccgtgcgc ctcaggtacc agtatagtaa tcagtaacgt cagcttccag
     6121 gttgtaatgc ttttaatttt gataaaa
//
LOCUS       DAZKGF010000227         6129 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZKGF010000227 DAZKGF010000000
VERSION     DAZKGF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.227"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            116..1336
                     /gene="astC"
                     /locus_tag="ACHOQV_004593"
     CDS             116..1336
                     /gene="astC"
                     /locus_tag="ACHOQV_004593"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFP9632855.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFAYRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNTINHRCGLFSEIRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSGGSS"
     gene            1333..2367
                     /gene="astA"
                     /locus_tag="ACHOQV_004594"
     CDS             1333..2367
                     /gene="astA"
                     /locus_tag="ACHOQV_004594"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFP9632856.1"
                     /translation="MMVIRPVERSDVAALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEESETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLAANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            2364..3842
                     /gene="astD"
                     /locus_tag="ACHOQV_004595"
     CDS             2364..3842
                     /gene="astD"
                     /locus_tag="ACHOQV_004595"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFP9632857.1"
                     /translation="MTLWINGDWVTGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVMAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGRWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            3839..5182
                     /gene="astB"
                     /locus_tag="ACHOQV_004596"
     CDS             3839..5182
                     /gene="astB"
                     /locus_tag="ACHOQV_004596"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFP9632858.1"
                     /translation="MNAWEINFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAS
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            5175..>6129
                     /gene="astE"
                     /locus_tag="ACHOQV_004597"
     CDS             5175..>6129
                     /gene="astE"
                     /locus_tag="ACHOQV_004597"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFP9632859.1"
                     /translation="MDNFLALTLTGKKTVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPRFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLML"
BASE COUNT         1296 a         1572 c         1862 g         1399 t
ORIGIN      
        1 cgccgtttat gcacttttat cactggctgg cacgaaccct gcaatctaca tttacagcgc
       61 aaacattact tattattaac atataaataa cgaattattt actgtagagg tcgctatgtc
      121 tcagccaatt acgcgtgaaa actttgatga atggatgata cctgtttacg ctccggcacc
      181 gtttataccg gtacgtggcg aaggttcgcg cttgtgggat cagcagggga aagagtatat
      241 cgacttcgcg ggtggcattg cggtgaacgc gctgggccat gcgcatccgg aactgcgtga
      301 ggcgctgaac gaacaggcga gtaagttctg gcataccggc aacggttaca ccaacgagcc
      361 ggtactgcga ctggcgaaaa aattgatcga cgccacgttt gcctatcgcg tcttcttttg
      421 taactccggt gcggaagcca acgaagcggc gctaaaactg gcgcgtaaat tcgctcacga
      481 ccgctacggc agccataaga gcggtatcgt ggcgtttaaa aatgcgtttc atggtcgcac
      541 gctgtttacc gtcagtgcgg gcggtcagcc agcctattca caggattttg cgccactgcc
      601 gccggatatt cgtcatgccg catataacga tattaactct gccagcgcgc tgattgacga
      661 ctctacctgt gcggtgattg tcgaacccat ccagggggaa ggcggtgtgg tgccagcaag
      721 caacgcattt ttacaaggtc tgcgtgaatt gtgtgaccgc cacaatgcgc tgttgatttt
      781 tgatgaagta caaaccggcg tcgggcgcac cggggaactg tatgcctata tgcactacgg
      841 cgtgacgccc gatctgttaa ctaccgccaa agcactgggc ggcggtttcc ccgttggcgc
      901 tctgttggca accgaagaat gcgccagcgt gatgaccgtg ggcacacatg gtaccactta
      961 tggcggtaat ccgctggcct cggcggtggc aggtaaagtg ctggatctca tcaacacgcc
     1021 agagatgctt aatggcgtta aacagcgtca cgactggttt gttgagcgtc ttaataccat
     1081 taatcaccgc tgcggtttgt tcagtgaaat tcgcggatta ggtctgctga ttggctgtgt
     1141 gctgaatgcc gattacgccg ggcaagcgaa acaaatttct caggaagcgg cgaaagcggg
     1201 cgtaatggtg ctgattgccg gtggcaacgt agtgcgtttt gcgccagcgc tcaatgtcag
     1261 cgaagaagag gtgacgaccg ggctggatcg ttttgcggcg gcttgtgaac actttgtgag
     1321 cggaggttca tcatgatggt catccgtccc gttgagcgct cagacgttgc ggcgctgatg
     1381 cagcttgcca gcaaaacggg cggcggcctg acgtcgctcc ctgctaatga agccacgctt
     1441 tcagcgcgta tcgaaagggc catcaaaacc tggcagggcg aactgcccaa aagcgagcag
     1501 ggttatgtgt ttgtactgga agagagcgaa acaggcactg tggcggggat ttgcgccatt
     1561 gaggtggcgg ttgggctgaa cgatccctgg tacaactatc gcgtcggcac gctggttcac
     1621 gcctcaaaag agctgaatgt ctataacgcg ttgccgacgc tgtttctcag taacgatcac
     1681 accggcagca gcgagctgtg cacgctgttt ctcgacccgg actggcgcaa agagggcaac
     1741 ggctatttgc tgtcgaaatc gcgctttatg tttatggcgg cctttcgcga caagtttaat
     1801 gacaaagtgg tcgccgaaat gcgcggggtg attgacgaac atggctattc gccgttctgg
     1861 caaagcctcg gtaaacgctt cttttcgatg gattttagcc gcgccgattt tctctgtggc
     1921 accgggcaaa aggcatttat tgcggaactg atgccgaaac atccgatcta tacccacttt
     1981 ttatcccagg aagcgcagga cgttatcggc caggtacatc cgcaaaccgc gcccgcccgc
     2041 gcggtgctgg agaaagaagg ttttcgctac cgcaactata tcgacatctt tgacggcggg
     2101 ccgacgcttg agtgtgacat cgaccgcgtg cgcgccatcc gcaaaagtcg gctggtggaa
     2161 gtggcagaag ggcagcctgc gcagggcgat ttcccggcct gcctggccgc caatgaaaat
     2221 tatcaccatt tccgcgtggt gctggtgcgt accgatccgg caaccgagcg tttgatatta
     2281 accgccgcac aactggatgc cctgaaatgc cacgccgggg atcgcgttcg tctggtgcgc
     2341 ctgtgcgcag aggagaaaac agcatgactt tatggatcaa tggtgactgg gttacgggcc
     2401 agggcgcatc gcgtgtgaag cgtaatccgg tatcgggcga ggtgttatgg caaggcaatg
     2461 atgccgatgc cgctcaggtc gggcaggctt gtcgggctgc ccgtgcggcg tttccgcgct
     2521 gggcgcgtct ctcctttggc gatcgtcagg taagagttga acgctttgcc ggattgctgg
     2581 aaagcaataa agccgaatta accgcgatta ttgccagaga aacgggtaag ccgcgctggg
     2641 aagcggcaac cgaagtgatg gcgatgatca ataaaatcgc gatatcaatt aaggcgtatc
     2701 acgttcgtac cggcgagcag cgtagtgaaa tgccggacgg cgcggcgagc ctgcgacatc
     2761 gcccgcacgg cgtgctggcg gtgtttgggc cgtataattt ccccggtcat ttaccgaacg
     2821 gacatatcgt tccggcattg ctggcaggta acaccattat ctttaaaccc agcgaactga
     2881 caccgtggag tggcgaagcg gtaatgcgtt tatggcagca ggctggcttg ccgccgggcg
     2941 tgctgaacct ggtgcagggc gggcgtgaaa cgggtcaggc gctgagtgcg ctggaggatc
     3001 tcgacggctt gctgtttacc ggcagcgcca ataccggcta ccagctgcat cgccagcttt
     3061 ccggtcagcc ggagaaaatt ctcgcccttg agatgggcgg taataacccg ctaattatcg
     3121 atgaggtggc ggatatcgat gccgctgtcc atctgaccat tcagtcggcg tttgtcactg
     3181 ccgggcaacg ctgcacctgc gcccgccgtt tattgctgaa aagtggaacg cagggcgatg
     3241 catttcttgc tcgcctggtt gccgtcagcc agcggttaac gccgggccgt tgggatgacg
     3301 aaccgcagcc gtttattggc gggctgattt ctgagcaggc cgcacagcag gtggttactg
     3361 cctggcagga actggaggcg atgggcggac ggaccctgct tgcgccgcgc ttattacaag
     3421 cagggacatc gttgctgacg ccggggatca ttgaaatgac aggcgttact ggcgtgccgg
     3481 atgaagaagt gtttgggccg ttattgcgcg tctggcgtta tgacaatttc gatgaagcga
     3541 ttcgaatggc gaataacact cgcttcggcc tctcttgcgg tctggtttcc cccgagcggg
     3601 aaaaattcga tcaactgttg ctggaggcac gggcggggat tgttaactgg aacaaaccgc
     3661 ttaccggtgc tgccagtacc gcgccattcg gcggcattgg cgcttccggt aaccatcgcc
     3721 ccagcgcctg gtatgccgca gattattgtg cctggccgat ggcaagtctg gagtcggact
     3781 cgttaacatt gcccgcaacg cttaaccccg ggctggattt ttccgatgag gtggtgcgat
     3841 gaacgcctgg gaaatcaatt tcgacgggct ggtagggctg acgcatcatt acgcgggcct
     3901 gtcgtttggt aatgaagcct ccacccgtca ccgttttcag gtctctaacc cgcgactggc
     3961 ggcgaagcag ggcttactga aaatgaaagc ccttgccgac gcgggattcc cccaggcggt
     4021 gatcccgccg cacgagcgtc cgtttattcc ggtgctgcgt cagttgggat tcagcggcag
     4081 cgatgagcag gtactggaaa aggttgcccg ccaggcaccg cactggcttt ccagcgtcag
     4141 ctccgcttcg ccaatgtggg tagccaatgc ggcaacgatc gcgccatctg ccgatacgct
     4201 ggatggcaaa gtgcatctca ccgttgccaa cctgaacaat aaatttcacc gttcgctgga
     4261 agcccccgtc accgaatcgt tgttaaaagc gatttttaac gacgaagaga aatttagtgt
     4321 ccattcggcg ttgccgcagg tagcgttgct cggtgatgag ggggcggcaa accacaatcg
     4381 tctcggcggt cattacggtg aaccgggtat gcaacttttt gtctacgggc gagaagaagg
     4441 caatgatacc cggccttccc gttatccggc gcggcagact cgcgaagcca gcgaggcggt
     4501 ggcaaggctg aatcaggtga atccccaaca ggtgattttc gcccagcaaa acccggacgt
     4561 tatcgaccag ggcgtttttc ataatgacgt gattgccgtg agtaaccgcc aggtgctgtt
     4621 ttgccatcaa caggcgttcg ctcgccagtc acagttactg gcaagcctgc gtgcgcgggt
     4681 gaacggtttt atggcgatag aagttccggc gactcaggtt tccgtgtcag atgcggtgtc
     4741 tacatatctg tttaacagcc aactgctgag ccgcgatgat ggttccatga tgttggtgct
     4801 gcctcaggag tgtcgggaac acgccggagt atggggttat ctcaatgaac tccttgccgc
     4861 tgacaacccg attagcgaac taaaagtctt tgatttacgt gaaagcatgg cgaatggcgg
     4921 aggcccggcg tgcctgcggt tgcgcgtggt attgacagaa gaagaacgcc gggcagtgaa
     4981 tccggcggtg atgatgaacg atacgctgtt taatgcgctc aatgactggg tggatcgtta
     5041 ctaccgcgat cgccttactg ctgccgatct ggccgacccg caattgctgc gcgaagggcg
     5101 ggaagcactg gatgtattga gccaattact gaatctcggt tcggtttatc cgttccagcg
     5161 cgagggaggg ggaaatggat aattttcttg ctctgacctt aacgggtaaa aaaacggtta
     5221 tcaccgagcg agaaatcaac ggcgttcgct ggcgctggct gggcgatggc gtgctggaac
     5281 tgacgccatt aacgccaccg caaggcgcgc tggtgatttc agcggggatc cacggtaatg
     5341 agacggcacc tgtggaaatg ctggacgcgt tgcttggcgc gatatctcac ggtgagatcc
     5401 cgttacgttg gcggttactg gtgatcctcg ggaatcctcc tgcgctgaag caagggaaac
     5461 gttattgcca tagcgatatg aatcgaatgt ttggcggtcg ttggcagcta tttgctgaaa
     5521 gcggggaaac ctgtcgggcg cgcgaactgg agcagtgcct ggaagatttt tatgaccagg
     5581 gcaaagaatc tgtgcgctgg caccttgatc tccataccgc aattcgtggc tccttgcatc
     5641 cgcggttcgg tgtattaccg caacgcgaca ttccctggga cgagaaattt ctgacgtggc
     5701 tgggtgcggc gggtctggag gcactggtgt tccatcagga gcctggtggt acgtttaccc
     5761 atttcagcgc cagacatttt ggcgcgctgg cctgtacgct ggaactcggc aaagcgttgc
     5821 cctttggaca aaacgatctt cgccagtttg cagtcactgc cagcgcaatt gctgcgctgc
     5881 tatctggtga gagtgtcggt atcgtgagaa cgccgccgct ccgttatcgg gtggtttcgc
     5941 aaattactcg ccactcgccg tccttcgaaa tgcatatggc aagtgacacg ctgaatttta
     6001 tgccgtttga gaaaggaaca ttgctggcgc aggacgggga ggaacgtttt accgtaaccc
     6061 atgatgtaga gtatgtgtta ttccctaatc ctttggtagc gttgggatta cgcgcgggat
     6121 taatgctcg
//
LOCUS       DAZKGF010000228         6085 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZKGF010000228 DAZKGF010000000
VERSION     DAZKGF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.228"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            422..565
                     /locus_tag="ACHOQV_004598"
     CDS             422..565
                     /locus_tag="ACHOQV_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFP9632860.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            578..787
                     /gene="cspE"
                     /locus_tag="ACHOQV_004599"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             578..787
                     /gene="cspE"
                     /locus_tag="ACHOQV_004599"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP9632861.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            953..1762
                     /gene="rlmA"
                     /locus_tag="ACHOQV_004600"
     CDS             953..1762
                     /gene="rlmA"
                     /locus_tag="ACHOQV_004600"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFP9632862.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1759..2325)
                     /gene="mntP"
                     /locus_tag="ACHOQV_004601"
     CDS             complement(1759..2325)
                     /gene="mntP"
                     /locus_tag="ACHOQV_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFP9632863.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2755..3213)
                     /locus_tag="ACHOQV_004602"
     CDS             complement(2755..3213)
                     /locus_tag="ACHOQV_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFP9632864.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(3268..4119)
                     /gene="manZ"
                     /locus_tag="ACHOQV_004603"
     CDS             complement(3268..4119)
                     /gene="manZ"
                     /locus_tag="ACHOQV_004603"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFP9632865.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(4132..4932)
                     /gene="manY"
                     /locus_tag="ACHOQV_004604"
     CDS             complement(4132..4932)
                     /gene="manY"
                     /locus_tag="ACHOQV_004604"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFP9632866.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(4995..5966)
                     /gene="manX"
                     /locus_tag="ACHOQV_004605"
     CDS             complement(4995..5966)
                     /gene="manX"
                     /locus_tag="ACHOQV_004605"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFP9632867.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKATPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
BASE COUNT         1622 a         1481 c         1456 g         1526 t
ORIGIN      
        1 tttgcgcatt catcagtttt tcttatttta aatttacaat accattgggg attgacttct
       61 ctttggggta attggtggcc gttaactgac tgttttatga gaaaaagtga tataactttt
      121 tattcattgc atagcagaaa atgtgatatt gcacgcacta tgtaataact tctcccactg
      181 gcctggaaca actgaactta ttgaactatg ttagaaaata cgtcagttta agtatctgcc
      241 tgaactggca aggttaagca caatgatata tcggcgcgta ttccgttgca taagtgtgca
      301 aaaaaagtgg aagacgtatc gagatttgtg cgtctgatcg agacatgttt aaaaatggct
      361 tgccataatt aacgttgtat gtgataacag atttcgggtt aaacgaggta cagttctgtt
      421 tatgtgtggc attttcagta aagaagtcct gagtaaacac gttgacgttg aataccgctt
      481 ctctgccgag ccttatattg gtgcctcatg cagtaatgtg tcagttttat ctatgttatg
      541 cctgcgggcg aagaaaacaa tctaaggaat ttttcaaatg gcaaagatta aaggtcaggt
      601 taagtggttc aacgagtcta aaggttttgg cttcattact ccggctgatg gcagcaaaga
      661 cgtgttcgta cacttctctg ctatccaggg taatggtttc aaaactctgg ctgaaggtca
      721 gaacgttgag ttcgaaattc aggacggtca gaaaggtccg gcagctgtta acgtaacagc
      781 tatctgatcg aatccactga tctgaagtgt gaatacgctt caatctcgct ataaagcctc
      841 gtcgaatgcg aggcttttta ctttgcttta tcttcgctcc tggcgttcgg atatttgccc
      901 gccgcgtgat tcgcgttaca cttgcggcct ttagtatcct gccggagttg tcatgtcgtt
      961 ttcctgtcca ctttgccatc agcctctttc gcgtgaaaaa aacagctata tctgtcccca
     1021 gcgacatcag tttgatatgg cgaaagaagg gtatgtcaat ctgctgcccg ttcagcataa
     1081 acggtctcgt gatccgggcg acagcgcgga aatgatgcaa gcacgccgcg cattcttaga
     1141 tgccggacat tatcagccgc tgcgtgatgc aattgtcgcc caactgcggg aacggcttga
     1201 tgaaaaggcc acagcggtgc tggatattgg ctgtggtgaa gggtattaca cacacgcatt
     1261 tgccgatgcg ctgcccgaaa tcaccacgtt tggcctggat gtctcgaagg tagcgataaa
     1321 agcggcggcg aaacgctatc cgcaggtcac tttttgtgtt gcttccagcc accgtttgcc
     1381 gttttccgat actagtatgg acgccataat acgtatttac gcgccgtgta aagcagaaga
     1441 attagcgcga gtagtgaagc ctggcggctg ggtcattact gccacgccgg gaccgcgaca
     1501 tttgatggag ctgaaggggc tgatttacaa tgaagtacat cttcatgcac cacatgcaga
     1561 acaactggaa ggttttacat tacagcagag tgatgagttg tgttatccga tgcgtcttcg
     1621 cggtgatgaa gccgtcgcat tattgcagat gacgccgttt gcctggcgtg cgaagccaga
     1681 agtctggcaa acactggcag caaaagaagt gttcgactgc cagacggact ttaatattca
     1741 cctctggcag cgttcttatt aaccgtggaa gtgcgtccag aggatctgga cgccgatgcc
     1801 gatcagcacc agcccgccga gaatttccgc ttttttccca ataattgagc cgataaagcg
     1861 accaaccatc atccctaatg tcgacataat caaggttgca caaccaatgg ccaatgcggt
     1921 cgcgataatg ttgacctgca ggaaggcgag accaacaccc acagccatgg catccaggct
     1981 ggtggcaatc gcggtggtta ccagtagcca gaaaccgtgc cgacggcgcg gctcttcatc
     2041 ttcatcatct gcgccacgaa aaccctcaat aatcattcgc ccgccgagga atatcagcag
     2101 cacaaacgca atccagtgat tccattcaag gacaaaccgg ctggctaaca tgcccattcc
     2161 ccagccgatc agcggtgtca gggtttcgac ggcaccaaaa ataaggccgg ttcgcaatgc
     2221 ttcagaaaat ttcggtttat ggagggtggc acctttaccg attgatgcag caaatgcatc
     2281 catcgacata ccaaacgcaa gaagaacagt agcagtgata ttcatgacaa cgtcctgacc
     2341 ggggtatcca taaaacacat catcgccccc agcaaacatg catacatgaa caacaagccg
     2401 cgatgggttt tcgcgttcat gcaggcatag cgattgatgt gcatatggtc tcgcctgact
     2461 gaaagtgttc agtccgtacg tgccacgttt tgcaacgagt atgttgacac gtacatttcc
     2521 ggaatctgga aatcggctac tccccaaatg agggcgcaac cttaacatat tttgagaata
     2581 taaaacaact gttggaatat attatttcat tgctttattg ataacgattt tcatttagat
     2641 ttatatatca atgaaacgtt aaaataaata acaaataagg gaagaggtaa tatagcctaa
     2701 gctatgtctg attattaaaa atacactaaa catagctttg gctaaattca taacttattg
     2761 agttgaaacg agaagcttat aaattttttc cagatcgtcg atatttcgaa cgcggattaa
     2821 cagccggcgc tgttctaatt gcatcaccaa cacgccatct tccgacaagt tcatcgcttt
     2881 gattcggcta tattcaatcc agacattggc gaagaaaaaa cctttttgtt taaagatgat
     2941 cttcggaacg cggatccaga atatataaaa acccatcaga gccagtgcac ttaataacca
     3001 tgttgttatt agcgcgccat gattcgtgac gttgttatag ataagaatga caatcaatcc
     3061 gacgaagata acgctatcga tgcgaccacg ccggagcaaa ggaattgcca gcagggtggg
     3121 gccgttacgg cggggcatga tgaactgatc gtagatcgcg aaggccagga gtgcggcgat
     3181 gaaaagaatc agtaccaggt ccgtgattgt cattaatcct ccagataaaa aaacggggcc
     3241 aaaaggcccc ggtagtgtac aacagtctta cagtcccagc aggccgcaag cgtaaccagc
     3301 gataccgatg acgaagaagc caacgatgat ccacagcggg ttaacttttt tgcgcaacag
     3361 ccacatacaa gcaaaggtca gcagcagtgg taccaggcct ggcattaact ggtcaagaat
     3421 agtctggaca gtggtaacgt gttctttgcc cgtctggtca gtaatgcgag agacaaccag
     3481 cgggatgttg acatgcgtcc acttgttaac taatgccccc atgacaaaca ggccgaggat
     3541 agacgcccct tccgtcagtt tttgcaggaa gccaccaccc atatctttaa cgatatcgat
     3601 acctttggag taaccatacg ctacgccgta gtaacgggtt gccagacgca ccaggttaaa
     3661 caagatgaag aacagcagcg gacctaacag gctgccgctc attgcgatac cggcccccag
     3721 tgctgcaaat accggacgta cggttcccca gaagatcggg tcgcctacac cagccagcgg
     3781 ccccatcaaa ccgactttga taccgttgat agcaccgtca tcgatctctg cgccattagc
     3841 acgctgttct tccagcgcca gggttacgcc gagaatcggc gcagccacga acggctgggt
     3901 gttaaagaac tccaggtgac ggcgaatagc ttgtttacga gcttcgttgt tctcagggta
     3961 gaggcgacga attgccggta ccatagagaa gcagaaaccc agtgcctgca tacgttcgaa
     4021 gttccatgaa ccctggaaga ggttagaacg caggaagacg ccacgaatat cactttgagt
     4081 gagttttttc tcggtggtag tttgagttgt atcaaccatt tcgctcacct gttagtccag
     4141 ttcgttatcg agatcgttgt taccagctgc ctgagcaggc gcaccggcta cgcggttgta
     4201 tttcgggcta agttggatgt agagcactgc cataacagta ccaatcacac ccagagcaac
     4261 caggttaaag ttggtgaatg ctgcggttac gaagccgagg tagaagaacg gcatcaggta
     4321 gccagcacgc atcatgttga taaccatcgc ataaccaacc accacgatca tgccaccggc
     4381 gatattcaga ccattggtca ccacttccgg aatcgcattc agcatgttct ggacttcgct
     4441 ggtaccaaca gacagcgcaa cgatgacggc cggaatagcc acacgcattg cttgcaggaa
     4501 cagagaagaa acgtggatcc aggaaatcgc tgtcaggttg ccgttatcag cagccttatc
     4561 cgcagcgtgc tggaaggcaa cggtaatagt acgaacgatg atggtcagta cctggcccgc
     4621 agcggccaga gggattgcca gtgcgatacc tgcaccaatg ctctgatgac ctgcgataac
     4681 cagaatagta gaaatgatgg aagccagagc ggcgtcaggc gcaactgcag caccgatgtt
     4741 catccagccc agcgcgatca tttccagcgt accaccgata ataataccgg ttttcatatc
     4801 gccaagaacg atacccacca gggtacacgc gattagcgga cggtgaaact gaaattcatc
     4861 gaggattgat cccatacctg cgatacaggc tacgataaat accagcacaa tttgaagagt
     4921 ggtaatctcc attgtacttc tcctgtttac gtaagactta agtgtgaaaa ctgagtgata
     4981 atcaacacaa tacgttactt atcgattttg ctgatcagat ccatcatttt cagtttcgga
     5041 tcggtggaaa ccttacggac ttccagctca ataccgcgcg cattcagttt cttgaacgcc
     5101 tcgatatctt tttcatcaac cgaaaccgcg ttattcacct gggttttacc ctgacggaat
     5161 gccataccac cgacgttaac agaggtgatt ttcacgccgc cttcaacgag acgctctaca
     5221 tctgttgggt tggtaaataa cagcattacg cgttcgccag catatttcgg gttgttgtag
     5281 acgcgaatca ttttggcaac atcaactacg tgtgctgtta cgcccggagg tgcaacctgg
     5341 gtgagcagtg tcttacgaac ggtatccgca gccacttcat cactaacaac aataatacgg
     5401 gagacattgg tttctttggt ccagcgggtg gcgacctgac cgtgtatcag acggtcgtcg
     5461 atacgcgcaa ggccaataac catgtagtcg tttggtccca ttggttttgc cggagttgga
     5521 gtcgcttttg gtgctgcggc agcgggtgct ggcgcggctt tttcaaccgg tttggctttc
     5581 agtgctttca cgccttcacg acctgtttct actgccagcg ccaccagttc atcaaagctt
     5641 gggtcatcat cacgggccat taacgtttcc acgagcattg gaatgttaac gcctgcaatg
     5701 acttcataat gctctttgtc gacgacaatg cggctggcag cattgaacgg gctgcctccc
     5761 catgtatcaa cgagaaacag cacgccttta gtggtgtcga gttttgccaa ctgagcgttg
     5821 tacttttcaa tcagcgtttc ggcattttca cctggaacga aatcgatcca gccgacgttt
     5881 tcctgctcgc ctaacagcat ttctgccgtt ttaagcaact gctctgcagc ccaaccatgt
     5941 gtgcctataa caatagcaat ggtcacttgc tacctccttt attatcgtta acacctcaac
     6001 gtgccagatg tatttttgaa tcgtcgtcca caatcgaatc gattcagata agggctacaa
     6061 ccaaaaaccc ctgcgtttcg cgatt
//
LOCUS       DAZKGF010000229         6001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZKGF010000229 DAZKGF010000000
VERSION     DAZKGF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.229"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            69..1620
                     /locus_tag="ACHOQV_004606"
                     /pseudo
     CDS             69..1620
                     /locus_tag="ACHOQV_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
     gene            1646..2098
                     /locus_tag="ACHOQV_004607"
     CDS             1646..2098
                     /locus_tag="ACHOQV_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFP9632868.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTFIPERLHKT"
     gene            2251..3525
                     /locus_tag="ACHOQV_004608"
     CDS             2251..3525
                     /locus_tag="ACHOQV_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9632869.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            3518..5619
                     /gene="mchF"
                     /locus_tag="ACHOQV_004609"
                     /pseudo
     CDS             3518..5619
                     /gene="mchF"
                     /locus_tag="ACHOQV_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
     gene            complement(5680..>6001)
                     /locus_tag="ACHOQV_004610"
     CDS             complement(5680..>6001)
                     /locus_tag="ACHOQV_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632870.1"
                     /translation="RNITTDELALIHGAGLDKDASVAIGTAAGGFLGKATKIPSADIA
                     GSAIGGYIGGVIADGSNRTITIPSNGINYNPSIGLGSFNPNYNGGLLNSGFNSSTSSS
                     SSGS"
BASE COUNT         1718 a         1149 c         1440 g         1694 t
ORIGIN      
        1 tgtcaggaca cttacttgct cttttatgaa cactctttta tgaaaattga aacggagaat
       61 actgccagat gagtcatcag tgttcacttt ctgaactgaa tgaaaacctg gtgcctttca
      121 ctgccaggca gatcaaggcc tcattaatct ggtgtgcaga ggatgtcaga aatccagacg
      181 agctgcaaaa tgcctgcagt tatattatcg atcctggcag tacagcttct gccaaagtgt
      241 tccatgcaga gcgctatggt ggcagtggta ttcagcgtaa tggaggtggt gcacgttgtg
      301 ggtttgatgg taactaccag gttaaaggaa taggaagtaa tccgttggtt ggcgaaggta
      361 ctgacgaacg tcattctaat ggtgcactcg gcgctgttca tgcaatatta tgaggctttg
      421 tggggagaag tactggctca aatattacct tatagtgctg tgcgggttcg ggcggtttta
      481 cttacagatc tctatactga aaaggcattt gagcgctccg gtaggaaatc acgaagagcc
      541 ctgttggtac gtgagcctgt tgttcgtccg gcgcattttg aacgggcacc ttacttccaa
      601 gtaaaaccgg agtattccag tctgctaatt cacgatgcct gtcgggttag atctgtgatc
      661 cacaagctgc caggatatct acctgtacca ccggaagaaa ttgatgctga agcacgaact
      721 gatccccgga tttattgcat tgaggggtta tgtgaactgg cacgtcgtga ggcctggcaa
      781 atggcatttt gtcgaacacg tttcctgaga ttgacaactt ctccttctaa tattgcaatg
      841 gatggcagat taatggattt taacggactc agttgtctgt ttccgggaga ttccccagct
      901 gattttgggt ataaactaag attagctgaa ctggcaaaag aaccgatggt acttatgcaa
      961 gggctgtctg atctctgctt gtatatcgga aaatatatgt ttgaccctga cttcactctt
     1021 gcagcccgtt tgaaggttga ggagatattt cagaaaactt tccatgaagc atgttattac
     1081 tgttatctag aactgttggg tattcctgga gaatttataa cacaaaaaga gatacctgat
     1141 atattgaaag aactggttaa cagttttgtt gcattactca atcaatactg cgagaaatca
     1201 catgcccaag atattgtcaa tcaggatggt tcaccattgc aaaagttggt tgtgacgcta
     1261 atccatcata ggcataatca aaagcaggca ctgaatagta gcatcaagaa tgatgtttat
     1321 ttcaccgttg cacaacagtg tttttcccaa gccatccact ggctgacgca aggcagtacc
     1381 agacgtcaga taaatgcctc attactcctg aaagaaattg aacatcatac catgaaaagg
     1441 ctgcaaccca gggaagagct gaggaaagaa aatatgtgcg aaaaaattgc catcctgctg
     1501 gataatcatg gcgatgatcc ccttttttta caagaagcaa tttctgatat gaaaaatttt
     1561 atgcttaagt tttccagaga tgcatttgga tatcttgaac cgataagaaa cacagtgtaa
     1621 aaaatatata agggaaatag taatcatgtc ttatataagg gaaaccatca gaggaaaaga
     1681 tgaatggact gtttatgaac agatcggttt tgcggtcagt tgtatgctct acaatcgtaa
     1741 ttacagtctg tatccggtgt taaccattca atactggact gaatatgcga tacagcataa
     1801 tcagattaaa ttcctgtttg attcacgagg ttttccactg gcgtatatta cctgggcata
     1861 tcttgaggct gatacagaag cgcgcctgct cagggatcca gaattcaggt tgcatccgtc
     1921 tgaatggaat gaagatggaa ggatttggat tctggatttc tgttgtaaac caggctttgg
     1981 tcgaaaagtt attgactatc tcatacagct tcagccatgg ggagaaggag aagtacgatg
     2041 gttaagcagg cgaaagaaaa ttgtgacatt catccctgag cggctgcata aaacgtagta
     2101 cctctgaaga tacagagata attgtaaatt acggggtaaa tgcatcgctg atactatttt
     2161 gacaggactc tgtattttct ggctttgatt atagcatttc gcttaaccag tgaaggtatt
     2221 acaggaaaag tgtgagtaag aggagctatt ttgtttcgtc aggatgcttt agaaaacaga
     2281 aaaatgaagt ggcagggacg ggcaatatta cttcccggaa taccactatg gttaatcatg
     2341 ctgggaagca tagtgtttat tacggcattt ctgatgttca ttattgttgg tacctatagc
     2401 cgccgtgtta atgtcagtgg tgaggtcaca acctggccaa gagctgtcaa tatatattca
     2461 ggtgtacagg gatttgttgt caggcagttt gttcatgaag gacagttgat aaaaaaaggg
     2521 gaccctgttt atctgattga catcagtaaa agtacacgta gtggtattgt cactgataat
     2581 catcgtcggg atatagaaaa ccagctggtt cgtgtggaca acattatttc ccgtctggaa
     2641 gaaagtaaaa aaataacgct agataccctg gaaaaacaac gcctgcaata cacagatgcg
     2701 tttcgtcgct catcagatat tatacagcgt gcagaggaag ggataaaaat aatgaaaaac
     2761 aatatggaga attacagaaa ctatcaggca aaagggctga ttaataaaga tcagttaact
     2821 aaccaggtgg cattatatta tcagcaacaa aacaatcttc tcagcctgag cggacagaac
     2881 gaacagaatg ctctgcagat aaccactctg gagagtcaga ttcagactca ggctgcagat
     2941 tttgataacc gtatctacca gatggaactg caacggtacg agttacagaa agaactggtt
     3001 aacactgatg tggagggcga aatcattatc cgggcgttga ctgacgggaa agttgactcc
     3061 ctgagtgtca ctgtcgggca aatggtcaat accggagaca gccttctgca ggttattcct
     3121 gagaacattg aaaactatta tcttattctc tgggtcccga atgatgctgt tccttatatt
     3181 tcggctggtg acaaagtgaa tattcgttat gaagcctttc cggcagaaaa atttgggcag
     3241 ttctctgcta cggttaaaac tatatccagg actcctgcgt caacacagga aatgttgacc
     3301 tataagggag caccacagaa tacgccgggc gactctgttc cctggtataa agtcattgcg
     3361 atgcctgaaa agcagattat cagatatgac gaaaaatacc tccctctgga aaatggaatg
     3421 aaagccgaaa gtacactatt tctggaaaaa aggcgtattt accagtggat gctttctcct
     3481 ttctatgaca tgaaacacag tgcaacagga ccgctcaatg actaacggga atttcagaca
     3541 aattataaat cagcttgata tgcgctggcg acgtcgtgtt ccggttattc atcagacgga
     3601 gaccgctgaa tgtggactgg cctgcctggc aatgatatgc ggtcattttg gtaagaatat
     3661 tgacctgata tctcttcgcc ggaagtttaa tctctcggcc cgtggagcaa accttgcagg
     3721 aatcaatgga atagcggagc agctggggat ggtcacccgg gctctttcac tggagctgga
     3781 tgaacttggt gccctcaaaa tgccgtgtat tctccactgg gatttcagtc acttcgtcgt
     3841 gctggtcagc gtaaagcgta accgttatgt actgcatgat ccggccagag gcagaagata
     3901 tctcggtcgg gaggaaatga gccggtattt tacgggcatt gcacttgagg tctggcccgg
     3961 aagtgaattc ctggcggaaa cccagcagac ccgcataagt ctccgttcac tgattaacag
     4021 tatttacggt attaaaagaa cactggcgaa aattttctgt ctgtcagttg taattgaagc
     4081 aatcaatctg gtaatgccgg tgggaactca gctggttatg gatcatgcga ttccggcggg
     4141 ggacagaggg ctgctgacgc ttatttctgc tggcctgatg ttctttatat tgctcagggc
     4201 cgcggtgagt atgctgcgtg catggtcctc actggttatg agcacgctca tcaatataca
     4261 gtggcagtcg ggtctgttta accatcttct cagactgccg ctggcgtttt ttgaacgccg
     4321 taaattaggt gatatccagt cgcgttttgg ctcccttgac actttgaggg ccacctttac
     4381 cacctgtgtt gttggggcaa tcatggacag tattatggtt gtgggtgttt ttgtgatgat
     4441 gctgttatat ggaggatatc ttacctggat agtgctcggt tttaccatgg tttacgttct
     4501 tattcgtctg gtgacatacg gctattaccg gcaaatatcg gaagaaactc ttgtcagggg
     4561 gcccgggcca gctcctattt tatggaaagc ctgtatggta ttgccacggt aaaaatccag
     4621 ggtatggctg ggattcgggg aacacactgg cttaacctga aaatagatgc gatcaattca
     4681 ggtattaagt taaccaggat ggatttgctc ttcgggggga taaatacttt tgttgccgcc
     4741 tgtgatcagg tggcgatttt atggctgggt gcaagccttg tgatcgataa tcagatgaca
     4801 ataggaatgt ttgtggcatt tggttctttt cgtgggcagt tttcggatcg ggttgcttcg
     4861 ctgaccagtt ttcttcttca actgagaata atgagtctgc ataatgagct cattgcagat
     4921 attgcactac atgaaaagga agaaaagaaa ccggaaattg aaatcgttgc tgacatgagc
     4981 ccggtttcac tggaaaccac tgatttaagc taccggtatg acagccagtc agcacaggta
     5041 ttcagtggtc tgaatttgtc tgtggctccg ggagaaagtg tggctataac tggtgcctcc
     5101 ggtgccggaa aaaccacatt aatgaaagta ttatgtggac tgtttgaacc agatagtgga
     5161 aaagtactgg ttaatggcac ggatatacgt caacttggaa taaataatta tcaccgtatg
     5221 atagcctgtg ttatgcagga cgaccgacta ttttcaggat caattcgtga aaatatctgt
     5281 gggtttgcag aagaaacaga cgacgaatgg atgacagaat gtgccaaagc aagtcatatt
     5341 catgatgtga taatgaaaat gccaatgggg tatgaaacgt taataggtga actgggggaa
     5401 ggtctttccg gcggtcaaaa acagcgtata ttcattgccc gagctttata ccggaaacca
     5461 ggaatattat ttatggatga ggctacaagt tctcttgata cagaaagtga acgtttcgtg
     5521 aatgctgcta taaaaaagat gaatatcacc cgggtgatta ttgcacatag agagacaact
     5581 ttaagaacag ttgatcggat ttttagaatt acaaattagg ataatatatc actaaaagca
     5641 gcattgctgc ataataagct atgctgcttt agaaattaat caacttccac tactagaaga
     5701 ggatgttgat gagttaaatc ctgagtttaa taaacctcca ttgtagtttg gattaaaact
     5761 tccaagtcct atactcggat tatagttaat accgtttgat ggtattgtta ttgtcctatt
     5821 tgaaccatca gcaataactc ctccaatata tcctccaatt gcacttcctg ctatatccgc
     5881 actagggatt tttgttgctt tacctaaaaa accaccagcc gcagtaccta ttgcaacgga
     5941 tgcatcttta tctaatcctg caccatgtat taaagcaagt tcatcggttg ttatatttct
     6001 c
//
LOCUS       DAZKGF010000230         5994 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZKGF010000230 DAZKGF010000000
VERSION     DAZKGF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.230"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(151..525)
                     /locus_tag="ACHOQV_004611"
     CDS             complement(151..525)
                     /locus_tag="ACHOQV_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632871.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDLTVVDAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
     gene            complement(1360..2589)
                     /locus_tag="ACHOQV_004612"
     CDS             complement(1360..2589)
                     /locus_tag="ACHOQV_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9632872.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            2844..4025
                     /locus_tag="ACHOQV_004613"
     CDS             2844..4025
                     /locus_tag="ACHOQV_004613"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFP9632873.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            4312..5148
                     /gene="kduI"
                     /locus_tag="ACHOQV_004614"
     CDS             4312..5148
                     /gene="kduI"
                     /locus_tag="ACHOQV_004614"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP9632874.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGIINIGGAGTITVDGQCYEIGHRE
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            5178..5939
                     /gene="kduD"
                     /locus_tag="ACHOQV_004615"
     CDS             5178..5939
                     /gene="kduD"
                     /locus_tag="ACHOQV_004615"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFP9632875.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
BASE COUNT         1590 a         1332 c         1494 g         1578 t
ORIGIN      
        1 taaatcgcat tctggcgcaa tgttcactgt cagggcgagc attcaccgga aaacgtcata
       61 caggcttaca ggcggcttaa aattcgatgg gcggaggacc ggatgggaat cgggagacct
      121 ggacaaaaag tgcaggcctc catgacacgg tcaggacagt ttgtgtttaa ccagaatatt
      181 tagcgcctgt attggggctt cgtggagctc ccgcagacat tcagggtcag taacatgttc
      241 ctggtatctt gttttctcaa gtaacaagcg gtgcatatgc agcatacggg caagagccgc
      301 gcacacctcc tcaaatgcgg tatcccgctt accgtcactg tcagagccac aaaacagcgg
      361 tacaacctcc acgccgtcgc tgtgggcctt tagccaggca tcaaccacgg ttaaatcatc
      421 ggtgaagaac cacgcactgt tagcgctgaa tcgcattttc caggcttccg gtactggttg
      481 cggggagtat gaatatgctg gtggtatctt gtcgtgagta tccataggct atcgttccgt
      541 cattctgtta ttcaccacag cgctaaaaac gccatgaagg accagaatac cgattgcctc
      601 gtttgctgta tgaggtgctt accacacatt gggtacacag atgaatcagg cggaagtgtg
      661 attaccggaa aaaacccctt tcagtttgac gaaaaaaaaa ccgcagtgtg ataacactac
      721 ggcacgccac caaagaaaca taacaaggaa tcagcaatga gtaatacctg taagccttcg
      781 gggtgattgt aacacatgag caatctttta ctggaagctg tttttcactg gatcacgtat
      841 cgttatgggg attcccatat cggagagcat ccaccagctt catagcggat aaccggaaaa
      901 attccggtaa atatttgcct gagtaagtca tcaagcgcct gctaaatgtg tatagaaatg
      961 tgtataggat tgattttaga atatgtggga atggctttat tgctggtggc atggcttgtt
     1021 gttctgttcc cttcgccgct ccaatttatc ttcacatcat ctacctgcca tttttgttgt
     1081 atctgatttt ctatattatc ttaattcaag cgcttcagac ttttcgcaat cgccgtaata
     1141 cttccccatt tttttgtaaa taaaaagcgt ttacgcaatg caatcgtcat gtatcttaaa
     1201 tagtcttatt taagatgttt agaactcttt gtgtgtttaa gtggctgata attatataat
     1261 attacgagag aagtgataga gcggaataat aacaaatgaa cattatgaaa actgggatgg
     1321 ttcaaaattg aagcaatttt gaaccatcca caacggatat tagctaaaga acattaccga
     1381 tacgcacagg atacccacga tcagggtaat cagatttccg atggaacgcc agggtttaag
     1441 cgccgggatg agatacgttg acagcgtagg catgatgaaa agtatcatgg caatgagcgg
     1501 gccgctgatc gcgtaaatca tcgaaatcgc gttcgggtta atgcaacaaa caatgaaggt
     1561 aatcagcgat accaacataa ttgatagtgc gcggttaaat gcacgacttt tctttacacc
     1621 aacctgctgt aatgttgttt tgacgacctc tgtggcacct tcaataacgc caaagtacgt
     1681 acccaggaat gatttcgaca tcgcaactac tgcgacaata atcccggaaa ttgacaacca
     1741 tgctggggcg ttcggcagca tagaaagcgc cgataaaatg gtaacccctt cttctttagc
     1801 cgcttcaata tacgaaggtg gaatagaaag caggcagcta aagacaaaga acaatacact
     1861 tatgcagatg atgagataag cgactttcat aatttttttg catttatcca tagcgtgttc
     1921 gccatatttt tcacgtctgt ctatggcaaa cgtagaaata atgggcgtat ggctaaaagc
     1981 gaaaaccatc acgggaatcg atatccatat ctggtgaagg gtattctgat tgaactctac
     2041 ctgggttgtt aatagatcag gttgccaact accgaccagg taaatagaaa gaaataagaa
     2101 ataggcaatc aatgggaata ccaaaaatcc cattacccga atagtggcat ggcgtcccat
     2161 cagaaaaatg agattcagga ttaatacaac ccccagactc accaacatac ggatgcgaag
     2221 atcaataacc atatgctttg ccagctgttc tgtaagtgag ttggtaattg ccactgcata
     2281 tatcaacacg acgacaaaaa aggcgatgaa gtacagcgtg gtaatcagat taccaatctt
     2341 cttgccatag tagtgtgtta ccgcgcccgt tatcccttca cctgctgatg ttttcgatga
     2401 gaggatgaac tggcataagg ctttatgtgg ccaatatgtt aaaggccagg cgaccagagc
     2461 agtaataaac aggaccacag cccccgccga acctaactga ataggaagga agagcgtgcc
     2521 tgcaccaacg gctgtgccgt agagcgcgaa actccacaga gtttcttctt ttgaccaaat
     2581 attagacatt atctgaacgt atcatctaca aattaaacaa aatggagggc aatttaccat
     2641 aaaccaggtt aagtatggga gattaacgcc ctttctacct ctctattggt gggttagtgg
     2701 ttgcaaacct tacgtgccag ttatgtctct gattgctact caggcatatt tcctttgtga
     2761 taaaaatcac tttttttaaa caattaagat aactgtaatt aacattcatt tactattgca
     2821 ctgttaattg gtgcgaggtt gtgatgaaag acgttgtgat tgtcggggcg ttacggacac
     2881 ccatcggctg cttccgtggt gcgttagcgg gtcattccgc cgtggaactt ggcagcctgg
     2941 tcgtgaaagc gttaatagaa cgtaccggcg ttcctgcata tgcggtggat gaagtaattc
     3001 ttggtcaggt gttgactgca ggggcagggc agaatccggc aaggcaatcg gctattaaag
     3061 gtggtcttcc taatagcgtt tctgcaatca ctattaatga cgtttgcggt tccgggctta
     3121 aagcactgca tctggctact caggcgatac agtgtggcga ggctgatatt gtcatcgccg
     3181 gtggccagga aaacatgagc cgcgcaccac atgttctgac tgatagccgc accggtgcac
     3241 agcttggcaa tagccagttg gttgatagtc ttgtgcacga tgggttgtgg gatgccttca
     3301 atgattatca tattggtgtc accgccgaaa atctggctcg cgaatatggc atcagccgtc
     3361 agttgcagga tgcttacgca cttagctcgc aacaaaaagc gcgagcggcg attgacgctg
     3421 gacgatttaa agatgagatc gtcccggtaa tgacccaaag taacggtcag acgttggttg
     3481 ttgataccga tgagcagcca cgcactgacg ccagtgcaga aggcttagcc cgtttaaatc
     3541 cttcatttga tagtctcggt tctgtgacag cgggtaatgc atcatccata aacgatggcg
     3601 cagctgcagt aatgatgatg agcgaagcca aagcacgagc gttgaattta cccgtgctgg
     3661 cccgcatccg cgcatttgcc agcgttggtg tagatccggc attgatggga attgcgccgg
     3721 tgtatgcgac ccgccgttgc ctggagcgtg taggctggca gttggctgat gtcgatctta
     3781 tcgaggctaa tgaagcgttt gctgcacagg cgctttcggt tggcaagatg cttgaatggg
     3841 atgagcgtcg tgtcaatgtc aatggtggcg cgatcgcact cggtcaccca ataggcgctt
     3901 ccggttgccg aatcctcgtt tctctggttc atgaaatggt gaaacgtaat gcccgcaaag
     3961 gactggctac gctttgtatc ggcgggggcc agggcgtggc attgaccatt gaacgtgacg
     4021 aatagctctg catttcctcc ttaccgataa atatcagccg tcattaatct gttgaataat
     4081 ggtggctggc gagtcgacat accttttcag ggctccctga tttttccatt ctcatgacat
     4141 attcgtgatc gacactgcac ttttttcagt ccttacctta aaaatgaaac attgttttat
     4201 ttttaattga aaaccctatc ccgtgggcat atcatcccct tatagagaaa acgaaacagc
     4261 gtttcactat cgttttctat tttcacgctt cactgattat cggaggttga tgtggacgta
     4321 agacagagca tccacagtgc gcacgcaaaa acgctggata cccaggggct gcgcaatgaa
     4381 tttttggttg aaaaggtatt tgtcgccgat gagtacacca tggtttacag ccacattgac
     4441 cgtattattg ttggcggcat tatgccggta actaaaacgg tttccgttgg cggggaagtt
     4501 ggtaaacaac tcggcgtaag ctatttcctt gaacgtcgcg agttaggtat tatcaatatt
     4561 ggcggtgccg gtacgattac tgtcgatggt caatgctatg aaatcggtca ccgcgaagcc
     4621 ctgtatgttg gtaaaggtgc aaaagaagtt gtctttgcca gtattgatac cgccactccg
     4681 gcgaagtttt attacaattg cgcacccgcg catacgacgt atcccaccaa aaaagtcaca
     4741 ccggacgaag tatctccagt cacgttaggc gataacctca ccagtaaccg tcgcacgatt
     4801 aacaaatatt ttgtcccgga tgtgctggaa acctgccaat tgagtatggg gctgacggag
     4861 ctggctccag gtaacttgtg gaacaccatg ccgtgtcaca cccacgagcg ccggatggaa
     4921 gtttatttct atttcaatat ggatgatgac gcctgcgttt tccacatgat ggggcaaccg
     4981 caagaaacgc gtcacattgt gatgcataac gagcaggcgg tgatctcccc gagctggtcg
     5041 atccattccg gcgtcggaac caaagcttat acctttatct ggggcatggt cggtgaaaac
     5101 caggtttttg atgatatgga ccatgtggcc gttaaagatt tgcgctagtt gtgggcataa
     5161 acgaataagg tattgttatg attttaagtg cattttctct cgaaggtaaa gttgcggtcg
     5221 tcactggttg tgatactggg ctgggccagg ggatggcgtt ggggctggcg caagcaggct
     5281 gtgacattgt tggcattaac atcgttgaac cgactgaaac catcaagcag gtcacggcgc
     5341 tggggcgtcg ttttttaagc ctgaccgccg atctgcgaaa gattgatggc attccagcac
     5401 tgctggatcg cgcggtagct gagtttggtc atattgatat cctggtgaat aacgccggat
     5461 tgattcgccg cgaagacgct ctcgagttca gcgaaaaaga ctgggacgat gtcatgaacc
     5521 tgaatatcaa gagcgtattc ttcatgtctc aggcagcggc gaaacacttt atcgcgcaag
     5581 gcaatggcgg caagattatc aatatcgcgt caatgctctc cttccagggc gggatccgtg
     5641 tgccttctta taccgcatca aaaagcggcg tgatgggcgt gacgcgattg atggcgaatg
     5701 aatgggctaa acacaacatt aatgttaatg cgatagctcc gggttacatg gcaaccaaca
     5761 atactcaaca actacgggca gatgaacaac gtagcgcgga aattctcgac cgcattccag
     5821 ctggccgttg gggactgccg agtgacctga tggggccggt agtgttcctt gcctccagcg
     5881 cttcagatta tgtgaatggt tataccattg ccgtggatgg cggttggctg gctcgttaat
     5941 tcatttctct tacttttatg accctgccgc atggcagggt tttttatacc tgta
//
LOCUS       DAZKGF010000231         5987 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZKGF010000231 DAZKGF010000000
VERSION     DAZKGF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.231"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            115..843
                     /gene="gadW"
                     /locus_tag="ACHOQV_004616"
     CDS             115..843
                     /gene="gadW"
                     /locus_tag="ACHOQV_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFP9632876.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(1206..4319)
                     /gene="mdtF"
                     /locus_tag="ACHOQV_004617"
     CDS             complement(1206..4319)
                     /gene="mdtF"
                     /locus_tag="ACHOQV_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFP9632877.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGDHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4344..5453)
                     /gene="mdtE"
                     /locus_tag="ACHOQV_004618"
     CDS             complement(4344..5453)
                     /gene="mdtE"
                     /locus_tag="ACHOQV_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFP9632878.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5561..5839
                     /locus_tag="ACHOQV_004619"
     CDS             5561..5839
                     /locus_tag="ACHOQV_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632879.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYIYQGNKVVFASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5840..>5987)
                     /gene="gadE"
                     /locus_tag="ACHOQV_004620"
     CDS             complement(5840..>5987)
                     /gene="gadE"
                     /locus_tag="ACHOQV_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP9632880.1"
                     /translation="LKNKNESNITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGI
                     TSYF"
BASE COUNT         1470 a         1497 c         1505 g         1515 t
ORIGIN      
        1 ataaaatttg gtcctaccaa atatggcagt ttttgcacac agaaacagtc tggcgtcatt
       61 tcattagtat actgacattg aaataatcgc agtaatgaaa tataagggat agtcatggct
      121 catgtctgct cggtgatcct cgttcgtcgt tcattcgata ttcatcatga acagcaaaaa
      181 atatcgttgc ataacgagag tatcctgctg ctggataaaa atttggcaga cgattttgcg
      241 ttttgttcac tggatacgcg acggctggat atcgaagagc tgacagtttg ccattactta
      301 caaaatattc gtcagttgcc acgcaattta ggattgcata gcaaagaccg tttgttaatt
      361 aaccagtcac cccccataca gctggtgacg gcgatttttg atagtttcaa tgacccccgg
      421 gtcaattcgc cgatactgag caaaatgctc tatctttcct gtttatcaat gttttctcat
      481 aagaaagaac tgatcccctt acttttcaat agtatcagta ctgtttcagg aaaagttgaa
      541 cgccttatta gctttgatat cgctaaacgt tggtatctac gcgatatcgc agaaagaatg
      601 tacaccagcg agagtctcat caaaaaaaag ttgcaggatg aaaatacctg tttcagtaaa
      661 atattactcg cctccaggat gtcgatggcc agacgattac tcgagttacg tcaaatacct
      721 ctgcatacta ttgcggaaaa atgtggctat agcagtacgt catactttat aaatacattt
      781 agacaatatt atggtgtaac gccacatcag ttttcgcaac attcgccggg taccttttcc
      841 tgacatattt cgcatttgaa tattggtcag gatctcacgt ctgcttcatg tgaaactctt
      901 tcctgatgat ttctgccggg ctaccggcta gttctctttc gcagtttgcc aggcggtata
      961 ggtattttca ggaggaaaag actcggcatg tttgggatta ttaagctaac aattcatacg
     1021 attacgggaa tgtgggtcag tattgtgctg ttcaaattga tgacgaatgg ctggagtggt
     1081 ttctactttc agtgctgtgt actaagcctg gtattcctga cggttagctg gttgttgagt
     1141 ggtgaatggt tagcaggaaa gagtaaggct gaaccctcac gttcaacctt actctcattt
     1201 acacgttacg cttttttaaa gcgggcaaag agatgttcca ctacaacgaa aaagaccgga
     1261 acgaagtaaa ttgccagcac tgttgcggca aacatcccgc ccattacgcc ggtacctacc
     1321 gcgttttgcg cgccagagcc ggcaccatga ctgataacca gcggcagcac gccgagaata
     1381 aaggccagag aagtcatcag gattgggcgt aaacgcatac gcgctgcttc gatgattgcc
     1441 tctaccggcg ttttcccttc tttctgcatc atctcaacgg caaattcgac aatcaggatg
     1501 gcgttcttgg cggaaagccc gatggtggtc agcaaaccaa cctggaagta aacgtcatta
     1561 cttaagccgc gcagatcggt ggccagtaat gcgccaacga cgcctaacgg aacaaccaac
     1621 atcaccgaga acggaattga ccagctttca taaagtgcgg cgagggcgag gaacaccacg
     1681 accagtgaaa tggcatatag cgcgggagcc tggtttgagg ataacgcttc ctgatacgac
     1741 agcccggtcc atgagtagcc gacgcctgcc ggaagttttg cgaccaggtc tgccataaat
     1801 ttcatggcgt caccggtact tttcccggca gccgcttcac ctaaaatctc cattgacggg
     1861 atgccgttgt agcgttccag tcgcggtgaa ccataggtcc attcggtaga cgaataggca
     1921 gaaagcggtg ccatcgtgcc agaggcgttg cgtacatacc attggttgat gttatccggc
     1981 aacatacgga acggcgtgcc tgcctggaca tacacttttt tcacccgccc ctggttgagg
     2041 aagtcgttca cgtagctgct gccgaaggcg gtggaaattg tctggttgat atcagacagc
     2101 gccacaccca tagcctcggc tttcgcagcg ttgacgttca ctttgaacat cggcgtatct
     2161 tccaggccgt tcgggcgtac cccgatgacc tgattcggtg attgcgctgc cagtgataac
     2221 agctcgtttc gcgcctgggt cagcttttcg tgccccagat tgccgttgtc cagcagttcc
     2281 atatcaaaac ctgacgcggt acccagttca gccaccgcgg gtaagttgaa cgggaagacg
     2341 acggctttat tgatactgct tagcgcaatc attgcccgct gaataatcgc ggtaaccgag
     2401 ttttcctcgc cgacacgttc agaccacggc ttgaggctga taaacgccag accgttgttt
     2461 tgcccctgac cactgaagcc aaagccacca acggtaaaca ccgactgaac attatttttc
     2521 tcttttgtca gataataatc cgtcacctgt tgtagcactt tcgtggtgtt aaccatggtt
     2581 gcaccggaag gtaactgcgc ggtggtcata aataccccct gatcctcttc tggtaagaaa
     2641 gaggtcggcg tacgcaggaa cagcaccgcc atcccggcgc aaatcagcag gtagaccacc
     2701 atgtagcgac cggtacaacg caacagcgag cgggtgctgt cggtatagtg ctgagttgat
     2761 ttttcaaaca gcgtgttgaa gcgtgcgaac agggcgttag gtttgtgatc gccctccggc
     2821 gcggctttca gaatggtggc gcacagggca ggggtcaggc tcattgctac aaatactgaa
     2881 agcagcatgg aggagatcag cgtgatggag aactggcggt agatctcccc ggtcgcaccg
     2941 ctcataaagg ccatcggcat aaacactgcg gaaagaacaa cggcaatccc gaccagcgca
     3001 cgttggatct gccccatcga tttatgcgtc gcttccttcg gcggtagctt atcttccgca
     3061 atgacacgct cgacgttctc caccaccacg atggcgtcat ccaccagtaa ccctatcgcc
     3121 agcaccatcc cgaacatagt taacgtgttg atggtgaaac cgaccgccga caagatcgca
     3181 aacgtcccga gaataaccac cggtacggca atcgtcggga tgattgtggc gcggaaattc
     3241 tgcaaaaaca gatacatgac caggaagacg aggatgatag cctcaaccag cgttttgaaa
     3301 acttcctgaa tagaaatttt gataaacggt gtggtgtcgt aaggataaac cgtcttcagg
     3361 cttgcaggga aataagccga taaacggttc agctcctctt tcaccgctcg cgaggtatcc
     3421 agggcgtttg ctccggtagc cagtttgatg gcgatcccgg cagcaggttt gccgttatag
     3481 cgcgccacgg tggaataatc ttccgcccca agttcgacgc gggcgacatc acgcagcagt
     3541 acttgcgaac catcttgctg aactttcaac aggattttgc caaattcttc cggcgtttgc
     3601 agacgcgtct gcacaatgat cgaggcgttc agttgctggt ctgccgcctg tggcatgcca
     3661 cccagttgac caccggaaat ctggttattt tgcaccttaa tctgggaaat aacatcggaa
     3721 ggtaccaggt tatatttatt gagtttttgc gggtccagcc agatacgcat ggcgtactcg
     3781 gaaccaaaga gctgtacgct accaacgccc gcggtacggc ttagcgggtc tttgatatta
     3841 gacgctacat agtccgcgat atcgtactgg ttgaggctgc cgttatcaga aataaatgcc
     3901 gctaccatca ggatattact gctcgactta tcgacgctaa tcccctgctg ctgcaccgct
     3961 tcaggtaatg aaggcatagc gagctgcagt ttattttgca cctgaacctg tgcgatatca
     4021 ggagatgtcc cagtctcgaa ggtcagagtg atagaggcat tacccgccgc atcactggtt
     4081 gaagacatgt acatcaggcc atcaagccca ttcatatttt gctcaatcac ctgagtgacc
     4141 gagtcttcta ccgtttgcgc atcggcaccc ggataggttg cgctgacggt aatggtcggt
     4201 ggcgcaatct gcggatactg cgcaaccggt aagttcatga tcgccagacc acctgcaagc
     4261 atcataataa tggcaagtac ccaggcaaaa accgggcgat caataaaata gttagccatg
     4321 aaagtcccct taagcctgca acgttattgt ttcgattcgg tgctggcgtt ttcctggctg
     4381 gaggaaattg ctcgtgcttt gatacccgga cgaatgcgtt gcaaaccgga aacgatcacc
     4441 cgatcgccag cctgcaagcc agaggtgaca acccactggt cgccgatggc tttgctggct
     4501 tcaatttcgc gtagctgcac aacatcgtct ttatccagaa tgagcgccgt tgctttaccc
     4561 tgggcgttgt gggtgacgcc ttcctgcggc accagtaata cattctggcg gctaccttca
     4621 tccactaatg ccgtgacgta catgccaggc agcaagtcac catttgggtt ggggaaaatc
     4681 gcccgtaacg tcacggagcc cgtggtttca tccaccgtcg ggtcggagaa tttcagcgtg
     4741 ccggtctggc tgtaacgttt accattttcc agattgagct gtactggcgt actgccctga
     4801 acctgtttga tttgcccact ggcgacctct tctttcatgc gcaagaaatc ctgcaccgac
     4861 tgcgtgagat cgacataaat cgggtccaga cgttgtacgg taaccagcga atctgcctga
     4921 ttagcggtaa cgagtgcgcc gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc
     4981 gaggtgacat ttgcgtattg caggttgata gtcgcctgtt caacagccgc tttggcgacg
     5041 gtgacattgg cttctgcttc attcaactgg gtgcgcgcgg tgtcgtaatc ctgacggcta
     5101 acgtagttgg tcttcagcaa cgatgcctgg cggttaaagg tgatgcgggc attgctggcg
     5161 gtagagagcg ctttcgccag cgagcctttg gcggagttca gctctgcctg taaaggcgca
     5221 ggatcaatct gatacagcga atcgccctgg ttcactttat cgccttcgat aaagttgcgt
     5281 ttaatgataa taccgcccac ctggggacgt atctcggcaa cttcataagg aacggttcta
     5341 ccaggcaatt cgctcaacac attgaccgaa ccgggggaga gtgtgacgac accgacctca
     5401 ggcgtcatgg cggcggcgtt ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg
     5461 cagaataaca acggtattaa cagctttctt cttctgttca ttttagtccc tgaaaattct
     5521 tgagaaataa cggttcttta aattgcgcgc gaggagagat atgagggatt tacagaccag
     5581 cggcatagtg gggttgtcag ccagtaaggt gggatacagg cacagtgatc gacatggtga
     5641 ggtcaacgac aaaaaaaatg ttgccgttct gccaacagtt cctgccagca ttcgggcaac
     5701 gaagggttct actggtggat acatatacca ggggaataaa gtcgttttcg cctctccttt
     5761 agtaatcacc gggggtaatg aaatcagcat ctgcatccct cgtcatgcca gccatcaatt
     5821 tcagttgctt atgtcctgac taaaaataag atgtgatacc cagggtgacg atgtcgctca
     5881 tacgtcttag tttcagtttg tacataatgt tgaatttctg aatctttaat gtttgttgcg
     5941 aaatattgag cgtcgacgtg atattgcttt catttttatt tttcaga
//
LOCUS       DAZKGF010000232         5974 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZKGF010000232 DAZKGF010000000
VERSION     DAZKGF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.232"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            169..450
                     /locus_tag="ACHOQV_004621"
     CDS             169..450
                     /locus_tag="ACHOQV_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFP9632881.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(550..1086)
                     /gene="ssb1"
                     /locus_tag="ACHOQV_004622"
     CDS             complement(550..1086)
                     /gene="ssb1"
                     /locus_tag="ACHOQV_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFP9632882.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            1341..4163
                     /gene="uvrA"
                     /locus_tag="ACHOQV_004623"
     CDS             1341..4163
                     /gene="uvrA"
                     /locus_tag="ACHOQV_004623"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9632883.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(4198..4554)
                     /locus_tag="ACHOQV_004624"
     CDS             complement(4198..4554)
                     /locus_tag="ACHOQV_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9632884.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4558..4974)
                     /locus_tag="ACHOQV_004625"
     CDS             complement(4558..4974)
                     /locus_tag="ACHOQV_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFP9632885.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(5085..5798)
                     /gene="aphA"
                     /locus_tag="ACHOQV_004626"
     CDS             complement(5085..5798)
                     /gene="aphA"
                     /locus_tag="ACHOQV_004626"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFP9632886.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1414 a         1570 c         1561 g         1429 t
ORIGIN      
        1 atctggctaa caaacaatca attgtgatat agttcacaaa attaatgaaa caaacagagt
       61 gtttcatttt tgtgtcctgc aagccccgcg atagccagag tgtaccacgc ctcccgtgaa
      121 caacgccgcg ctgtccaggg catcggctct tttcagagga attgtttgat ggctaccctc
      181 accactggcg tggttctact tcgctggcaa cttcttagtg ccgtaatgat gtttctggcc
      241 agcacgctca acatccgttt tcgtcggtct gattatgtcg ggcttgcagt gatcagcagc
      301 ggtctgggcg tggtttctgc ctgctggttc gcaatggggt tgcttggcat cacaatggcg
      361 gatatcaccg ccatctggca caacatcgag tcggtgatga ttgaagagat gaatcagaca
      421 ccgccacaat ggccaatgat tttaacttga tatgtagaag cctccataat ggaggcttct
      481 ttttttatgg ctggcagatg ctttaatcat ccaccttaaa acaatataac ctattgtttt
      541 aatgacaaat cagaacggaa tatcgtcatc aaaatccatc ggcggctcgt tagacggtgc
      601 tgccggagcg gactgctgcg ggcgagactg cgcgccgccg ctgaactgat tgccgccctg
      661 cggctgctga ggctgacccc aaccgccctg cggctgacca ccaccgatat tgccacctgc
      721 aggagcgcca ccaccctgac gaccacccag catctgcatg gtgccgccaa cgttcaccac
      781 gacttctgtg gtgtagcgat cctgaccgga ttgatcggtc catttgcggg tacgcagctg
      841 accttcgata taaacctgag aacctttacg cagatattcg ctggctactt ctgccagttt
      901 gccgaacagc acaacgcggt gccactcagt ctgctctttc atctcgccgg tcgctttgtc
      961 acgccaggat tcggaagtag ccagcgtaat gttggcaaca gcgccaccat ttggcatgta
     1021 gcgtacttcc gggtcctgac ccagattacc aacgagaata accttgttta cgcctctgct
     1081 ggccatgttc gtgtctcctg aaaaaaaatc gttctgaata agtgtaaacg cgcgattgta
     1141 ccattaccaa tagcgctttt actatgttgt gacctcggtt ccgggaaaca aacctggcca
     1201 gacattgtta cacaacactc cgggtaatgc attccaatac tgtatattca ttcaggtcaa
     1261 tttgtgtcat aattaaccgt ttgtgatcgc cggtagcacc atgccaccgg gcaaaaaagc
     1321 gtttaatccg ggaaaggtga atggataaga tcgaagttcg gggcgcccgc acccataatc
     1381 tcaaaaacat caacctcgtt atcccccgcg acaagctcat tgtcgtgacc gggctttcgg
     1441 gttctggcaa atcctcgctc gctttcgaca ccttatatgc cgaagggcag cgccgttacg
     1501 ttgaatccct ttccgcctac gcgcggcagt ttctgtcact gatggagaag ccggacgtcg
     1561 accatattga ggggctttct cctgccatct caattgagca gaaatcgacg tctcataacc
     1621 cgcgctctac ggtggggaca atcaccgaaa tccacgacta tttgcgtctg ctgtacgctc
     1681 gcgttggtga gccgcgctgc ccggaccacg atgttccgct ggcggcgcaa accgtcagcc
     1741 agatggtgga taacgtgctt tcgcagccgg aaggcaagcg tctgatgctg ctcgcgccaa
     1801 tcattaaaga gcgcaaaggc gaacacacca aaacgctgga aaatctggca agccagggtt
     1861 acatccgtgc tcgtattgat ggcgaagtct gcgatctttc cgatccgccg aaactggaac
     1921 tgcaaaagaa acataccatt gaagtggtgg ttgatcgctt caaggtgcgt gacgatctca
     1981 cccaacgtct tgccgagtcg tttgaaaccg cgctggagct ttccggtggt accgcggtag
     2041 ttgccgatat ggacgatcca aaagcggaag agctgctatt ttccgccaac ttcgcctgcc
     2101 caatttgcgg ctacagtatg cgtgaactgg aaccacgcct gttttcgttt aacaacccgg
     2161 cgggtgcctg cccgacctgt gacggccttg gcgtacagca atatttcgat cctgaccgcg
     2221 tgatccagaa tccggaactg tcgctggcag gtggtgcgat ccgaggctgg gatcgccgca
     2281 acttctatta cttccagatg ctgaaatcgc tggcagatca ctataagttc gacgtcgaag
     2341 cgccgtgggg cagcctgagc gcgaatgtgc ataaagtggt gttgtacggt tctggcaaag
     2401 aaaatattga attcaaatac atgaacgatc gtggcgatac ctccattcgt cgtcatccgt
     2461 tcgaaggcgt gctgcacaat atggagcgcc gctataaaga gacggaatcc agtgcggtac
     2521 gtgaagaatt agccaagttt atcagcaatc gtccgtgcgc cagctgcgaa gggacgcgtc
     2581 tgcgtcggga agcgcgccac gtttatgtcg agaatacgcc gctacctgct atctccgaca
     2641 tgagcatcgg tcatgcgatg gaattcttca acaatctcaa actcgcaggt cagcgggcga
     2701 agattgcaga aaaaatcctt aaagagatcg gcgatcgtct gaaattcctc gttaacgtcg
     2761 gcctgaatta cctgacactt tcccgctcgg cagagacact ttctggcggt gaagcccagc
     2821 gtatccgtct ggcgagccag attggtgcgg gcctggttgg cgttatgtac gtgctggacg
     2881 agccgtctat cggcctgcac cagcgcgata acgagcgcct gctgggtacg cttatccatc
     2941 tgcgcgatct cggtaatacc gtgattgtgg tggagcacga cgaagacgcg attcgcgccg
     3001 ctgatcatgt gatcgatatc ggtccgggtg cgggtgtaca cggcggtgaa gtcgtcgcgg
     3061 aaggtccgct ggaagcaata atggcggtgc cagagtcgtt gaccgggcag tacatgagcg
     3121 gtaaacgcaa gattgaagtg ccgaagaagc gcgttccggc aaatccggaa aaagtgctga
     3181 aactgacagg cgcacgcggc aacaacctga aagacgtgac gctgacgctg ccggtgggtc
     3241 tgtttacttg catcacaggg gtttcaggtt ccggtaaatc gacactgatt aacgacacac
     3301 tgttcccgat tgcccaacgc cagttgaatg gtgcgaccat cgccgaaccg gcaccgtatc
     3361 gcgatattca gggactggaa catttcgaca aagtgatcga tatcgaccaa agcccaattg
     3421 gtcgtacccc acgttccaat ccggcaacct ataccggcgt gtttacacct gtacgcgaac
     3481 tgttcgcagg cgtaccagaa tcccgtgcgc gtggttatac gccagggcgt ttcagcttta
     3541 acgtccgtgg cggacgctgc gaggcctgtc agggcgacgg cgtgatcaaa gtggagatgc
     3601 acttcctgcc ggacatttac gtaccgtgcg accagtgcaa aggcaaacgc tataaccgtg
     3661 aaacgctgga gattaagtac aaaggcaaaa ccatccacga agtgctggat atgaccatcg
     3721 aagaggcgcg tgagttcttt gatgcggtgc cagctctggc acgtaagctg caaacgctga
     3781 tggatgttgg cctgacgtac attcgcctcg ggcagtccgc aaccacactt tctggtggtg
     3841 aagcccagcg cgtgaagctg gcgcgtgagc tgtcaaaacg cggcaccggg cagacgctgt
     3901 atattctcga cgagccgacc accggtctgc acttcgccga tattcagcaa ctgctcgacg
     3961 tactgcataa actgcgcgat cagggcaata ccattgtggt aattgagcac aatctcgacg
     4021 tgatcaaaac cgctgactgg attgtcgacc tgggaccgga aggcggcagt ggcggcggcg
     4081 aaatcctcgt ctccggtacg ccagaaaccg tcgcagagtg cgaagcttcg catacggcgc
     4141 gcttcctcaa gccgatgctg taatcgttaa ggccgctttc agagcggcct tcttctttca
     4201 gagttgcacc agcaatttac gtttttcttc cggcagcaaa ttcaccgcct gctgataaga
     4261 cgcatccacc agataataga tttgcgaatc cggcagcgaa ccgtcgagat acacggtgct
     4321 ccagtgcgct ttattcagat ggcggcttgg acgcacatcg ctgtgctgct gacgtagcag
     4381 ctccgccagc tccgggctgg ttttcagcga aacagctggg cgattttcta cttctttcac
     4441 catcgcaaac agcacatctt ccactttgat ctgcgtcgct ttccagtcgt tatgcacgct
     4501 ctgttctgcg cctggttttg ccatgcaata ttgtagcaac tccgaaatgg tcatttttca
     4561 ctccccttgt agtgtcgcga tgatgcgacg cgatccgccg tggatgcgat gttcccccag
     4621 ccagatgcct tgccaggtgc cggtctgaat acgcccttta tgcaccggca atacaagcga
     4681 tgttcccagc attgaggatt tgatatgaga aggcatatcg tctgctccct catagtcatg
     4741 ctcataattt ccgttatcgg gaacggcgcg gaggaaaaaa cgctccatgt cgtggcgtac
     4801 ggtgggatcg cagttctcat taagtgtcag agaggcggag gtatgttgca gcaacagatg
     4861 cagtaaaccg atgttaacgc gcggcatatc agccagctga ttcagaattt catccgttac
     4921 cagatgaaac ccacgagatt tggcgctaag cgtgagcgtc ttttgatacc acatgtgctg
     4981 ctccttgata aaactctctt aatcagtttg cagcaagaca gcgaaaggat aaaggtgtga
     5041 ttaaaaaaac agcattcggg agagtattac gctctcccgc tctgtcagta ttctgaattg
     5101 acgatcacct cttcaccaaa cgcacccgct tgtggcaagg gtttgtaggt agagttggag
     5161 gcgcgcagaa tgcggatacc acgagcgccg acatcgcgtg cggcggtaat atcgttatcg
     5221 gaatcaccat aaaaaattcg gatattttta tcctgcagcc attgcgattt tgtattttgc
     5281 cctggtttat cgcccgcaaa gatcaccgga ttcatgttgg tggcaggaat atgaaaatta
     5341 tccgccagcg tttttgaaac cgtttctgtt ttggtcgggc tacgaccagt cacaaagaag
     5401 atcgcgtcac cgcggcgtac atgcatatca atcagctggc gagcgacctc ttttggaatg
     5461 ctgaattcat cccagccatt gttcattttt tcccagaaca caggattttt cagataatct
     5521 tcgctttctg gcgagaaggt ttttttgccg cgccagaagc ccggactgga aaaaagtacc
     5581 gtgtcatcga tatcaaaccc caccgccatt ggcggacgcc ctgcgaggct attttcaatt
     5641 tgtgcgaccg aaacccaatg aattggtgcc tgttcagcaa gcctggcaac gttagtacca
     5701 gggttaagcg gtgaaggaga tgaggccagg gcaacagcgg aactgtttag agcgaacaat
     5761 aagcaaacgg cactgattgc ctgtgtgatc ttgcgcatat ttttccctaa atagtcagta
     5821 tgttgaaact ttttattgtg agatttgttg caaaaaccat ctgaccataa cgccagcagc
     5881 cggggaaagg aaggattatc tgcggttttt gtgatgaaaa gcaggatgat tatatttaac
     5941 ccagtaaata cgttcctgtc gttttttgta aaaa
//
LOCUS       DAZKGF010000233         5958 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZKGF010000233 DAZKGF010000000
VERSION     DAZKGF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.233"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            219..800
                     /gene="mdaB"
                     /locus_tag="ACHOQV_004627"
     CDS             219..800
                     /gene="mdaB"
                     /locus_tag="ACHOQV_004627"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFP9632887.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     ESDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            831..1145
                     /gene="ygiN"
                     /locus_tag="ACHOQV_004628"
     CDS             831..1145
                     /gene="ygiN"
                     /locus_tag="ACHOQV_004628"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFP9632888.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1190..2077)
                     /locus_tag="ACHOQV_004629"
     CDS             complement(1190..2077)
                     /locus_tag="ACHOQV_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9632889.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTMLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2074..3021)
                     /locus_tag="ACHOQV_004630"
     CDS             complement(2074..3021)
                     /locus_tag="ACHOQV_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9632890.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAGLTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(3032..4069)
                     /locus_tag="ACHOQV_004631"
     CDS             complement(3032..4069)
                     /locus_tag="ACHOQV_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9632891.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4078..5061)
                     /locus_tag="ACHOQV_004632"
     CDS             complement(4078..5061)
                     /locus_tag="ACHOQV_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9632892.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILIFDEQTLLAMRTWLAGDLAGQDWVTLGTSAWFSLGGFVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5058..5867)
                     /locus_tag="ACHOQV_004633"
     CDS             complement(5058..5867)
                     /locus_tag="ACHOQV_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9632893.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         1688 a         1621 c         1366 g         1283 t
ORIGIN      
        1 actactttat catttacata atacatctct cctgtgggaa cttataggaa gttgatttca
       61 gtattgtcat tttcagaaaa tatttatccc gcatcggtga atcagagcaa gatcagactt
      121 ttgctaaatt cgcaaaagac tttgcacatt ttgctaattt caccgtaccg ctctgtgacg
      181 tactatagtc gacaaacgtc tcaccttgag gttaaaaaat gagcaacatc ctgattatca
      241 acggcgcgaa aaaattcgcc cactccaatg gtcaactgaa cgacaccctg accgaagtcg
      301 cggatggcac actgcgcgac ctcgggcatg atgtccgcat cgttcgcgcc gagagcgact
      361 acgatgtcaa agccgaagta caaaactttc tctgggctga tgtggtgatc tggcagatgc
      421 ctggctggtg gatgggcgcg ccgtggacag tgaaaaaata cattgatgat gtattcaccg
      481 aaggtcacgg gactctgtat gccagcgatg gtcgtacccg caaagatccg tcgaaaaaat
      541 acggttctgg cggtctggta caaggtaaaa aatatatgct ttccctgacc tggaacgcac
      601 caatggaagc tttcaccgaa aaagatcagt tcttccacgg ggttggcgtt gacggtgtgt
      661 atctgccgtt ccataaagca aaccaattcc ttggtatgga accgctgccg acatttatcg
      721 ctaatgacgt gataaaaatg cctgatgttc cccgctatac tgaagaatat cgcaagcatc
      781 ttgtggaaat ttttggttaa ctagagctct ggctttagaa ggagttaacc atgattacag
      841 taatcgcaga aatccgtact cgtcctggtc aacatcaccg tcaggcagtg ttggatcagt
      901 ttgctaaaat cgttccaacc gtactgaaag aagaaggttg ccacggctat gcgccaatgg
      961 tggattgcgc tgctggcgtg agtttccagt ctatggcacc ggattctatc gtgatgattg
     1021 agcagtggga aagcatcgcg catcttgaag cgcatctgca aaccccgcac atgaaagcat
     1081 atagcgaagc cgtaaaaggt gacgtgctgg agatgaatat tcgtatcctg cagccggggc
     1141 tttgacattt ctaaaaagag gccggtgctg ctaccggtct cagcccagat tagccattct
     1201 ttcttcccgt tttcagtcct cgatggaaat cgttaattcg cttaatggtt ttaaccgcac
     1261 gctgcggcac tgcggaagct accgaccaga caatcatttc cagacacaac aataccgtac
     1321 catgtagcgg cgttttccct ttttcatccc cgcgcggaac atgaatcacg atactggcgt
     1381 ctttgctaaa acgtgaatcc agcgcgtttg tcagcaaaat aacagggatc cccagccgcc
     1441 tcgcctcacg aagcattgtc agcccttccc ggtgcgcaga tttttgcgcc atcatgatca
     1501 gaacatcgcc acgttgaagc gcgatcagtt gctcggcaag gccaataccc gtacggttta
     1561 gtgccgtggc aggcaaacct atgcggttga atagtcgtgc ggtatagtcc gccagaatac
     1621 ccgaagcgcc aatgccaaaa atagcgactt gccgtgcctg caccaacaac gctacagctt
     1681 gcgcaagcgc gtggcggttt tcaggctctg acaggacatt acaggtatac aaatgccctt
     1741 caagaacaaa gtcgattgcg gtgttaacat cgctcgtcaa gttactcacc gtcgtagaca
     1801 tcttttcgct ggaactaagg gctggaccaa gccactgttc cagagtgcgt ttcagatcac
     1861 gcagcccggc aaaccccagg gcctgtatag ccctgataac tgtggcatcg gaagtgttca
     1921 acgtggccgc aatttccatc gccgtttgct cgagtaccac ttcacgatgc tcattaatgt
     1981 agctggcgac aactcttaac ccaggcgtta actgactcgc gcgagtgcga aaacgctcac
     2041 caaaaatatc aacccgcact ttatctttct ggatcattta gcctccggta gaggtcgccc
     2101 tgcaatttgt gattgtatct gcgcagccat cagccccaga gatgcaaagg agttagaaat
     2161 tgcctcttca cgagaaatca gcacataacg cccggaacga caggcagtca gaaactgaca
     2221 ccatcccggc atgacttttt ccattgctgc cagctcatcc tgaggtttac caccggtgtc
     2281 accgcgccag gttgcaaaaa caaaatcggc atccagttca ggtaaacgct ccgcgctcac
     2341 atccattcgc ccaccttcag gaatgctctc aatcagcggc ggaaaacgaa aacccgcatc
     2401 gcgtaatacc cgccccagcg aatgatagct atgcatcaca ttaatttttc cctggtttgc
     2461 ctgaatcacc gataccgtta ttttttggct gtccagcgtc gccttcagcg cattgatttg
     2521 tgcctgatag cggcgttcaa gtatcgccag ctgcgactgc gttcctgtaa gtcctgccag
     2581 cttgcgatag atttctggcg cacccccttt gagatgatca atgctcaccg tgggcgcaat
     2641 tttttcaagt tgttcaatgg gcgtattacg ggtcggttct gtgatgatca ggtcaggctt
     2701 tgccgccaca atcgcttcaa tatcaatatc agcggtacca ataaaggcga tcgatgaatt
     2761 atcaaagtcg acacctgtca ataacgcgcc agatcgaatg aaatggctac cgtcaggccg
     2821 agttcgcccg tggctggcga caggaggtac accgagctca atcagaggaa tagtgatatc
     2881 aagatcgtgt aaagagacaa ttctctttgg atgagcagga acagtgactt tgcgccctaa
     2941 atcatcagta aatacctgaa caggttctgt tgcacgggaa aaaaatgaca acagtatcag
     3001 cagagaaaaa aataaacgca tataactccc gttaaaaact gttcctgcgc tgccagagca
     3061 gtagcaaaaa aaatggtcca ccaatcagtg agataatgat tccggcaggc agttgcaaag
     3121 gcaaaaaagc caaacgacca atgctatccg ccagaataac taacaacgcc cccgataccg
     3181 cgctccccag cagtaatgtc gtctgcccgc cacgtagaat aaaacgtgat atatggggtg
     3241 ctatcaatcc gacaaaaccg atgttaccaa cacacgaaac gcagacggct gtcaggatta
     3301 ttggtgcggc gacacgtaac aacgccagac gagaactatt cacccctaac ccggttgcaa
     3361 cctgatgacc aagcagagcg atatccgccg tcctggcggt gaacagcaat aaaagagcgg
     3421 ctggcaacat ccaacaggct gaaataccga ctaacatcca gttagctgca tgcaggcttc
     3481 ccgataacca catcaatgca gtttgtacat ctcgaacatc tgcagtggtc ataaatacac
     3541 cgataccggc ggcgaaaaac caggaaacac caataccaat cagaacaaat cgcgggcggg
     3601 aaatatcgcg cgcacaaaaa atcacgatta aagccaccaa caagccacca gcaagaccag
     3661 ccactggacg ccagaacatt cccagcattg gaaactggaa aatcagccac agcaccgcca
     3721 cgctacatcc ttctttgacg ccgattaaac caggatcggc aaggccgtta cgcgcaatgg
     3781 attgcatcgc ggcccccgcc atacctaaca tggctccgca tagcaatgcc atgatgactc
     3841 tcggcaagcg aatatcctgc acaatataac gtgcgtctgt gcttacgttt tctggagcaa
     3901 acaacgctcg tcctacttcc gatgccggga cagaaaaaga accatgcatc agaccgaata
     3961 tcagtattcc aaccgcaaac agaaacagcc caccaagcaa caccagattt ttagggcgta
     4021 ccagtgtcga aaacttacca acacgtagtg ggcgtagccc gacgttcatt atccgactca
     4081 tttgaacatc ctcgtcgcca taatgacaaa aacaggcgca ccaactaacg ccgtcatgac
     4141 accggtcgct aactcatggg gcgtgaaaag cgtccgagca ataatatctg ccagtaataa
     4201 caaaagcgcg ccgacacaag ctgaaagagg gagcagaacg cgcagatccg ctgataccag
     4261 acgccggaca atctgcggta ctagcaggcc gacaaaacca atggggccag caatcgatac
     4321 ggctgcgcca cagagcaacg cgatcgcaag cagggtgaat gttcgcgtgc ggagtaccga
     4381 gacgcccagc ccctgcgcca tgcgatcgcc cagtgccaac atgttaagag agggcgcaag
     4441 gtaaatggct aacacaaatc cccccagaga aaaccaggcg gacgtaccca gcgtcaccca
     4501 atcctgcccc gctaaatcac ctgctagcca tgtccgcatc gccagcagtg tttgttcgtc
     4561 aaaaatcagg attgcagccg ttattgagga cacgaacgcc gatagcgcca cgccgcataa
     4621 agtaactttc attggcgtta accctgaacg ccctgcggag gaaagcagca atatcaacag
     4681 gaacaataac gcgccccccg tagatgccag cagtgggcgt ccaaccggaa aggccagtcc
     4741 aagcgcactg gcggcaacga cagcaagagc ggctccggca tttaagccta aaatatgtgg
     4801 ctcccccaat gggttacgaa tgacagcctg caacaaagca ccagcaacac caagagaagc
     4861 accggtaagc aatgctgccg ctaaacgcgg caaacggagt cttacaatta cgttgtgatc
     4921 gaagttttgc ggatcaaaat gaagaaatgc ctgcaacacc gtttgtggtg caataaatcg
     4981 ggcaccaatc cctggatata ccacgcatcc caccagcagt aaggcgagga aaattacagt
     5041 aaccagccct gtgcgcatca tactttttct acaccacgaa acggcatgaa gaacggcttt
     5101 cccgtcagag gatttattga tgcgtgaaca tcaacgtcaa agacagcttt taccagttca
     5161 ggcgtgcaat gctccccctc attcaacaca cgcaccactt tcccctggcg taaaaatatc
     5221 aacgtatcgc cataattgac ggcaaaattc agatcatgca acaccacgac gactgtacgc
     5281 ccgtgatgac gcgttaaatc atgtaataac tcaagaattt caacctgata acgtaaatca
     5341 agccaggttg tgggttcatc cagcaggata taaggcgtct tttgcgccaa caccatcgca
     5401 atccagcaac gctggcgctg cccaccagag agtttttcaa caggtaaatg tgcaaactcc
     5461 tgcgtaccgg ttaatttcag agcctcttct accgcctgct cgtcggcgtc gctccattga
     5521 cgaatgaaat tctgccaggg aaaacgccca cgcgacacca gttcataaac ggttaagccc
     5581 tccggcaata atggcgactg cggcaagatc ccaagtctac gggataacgc tttcgttggt
     5641 tgctcgtgaa tcgctttgcc atcaagcaat atcgaaccgc ccataggttg caagatacgt
     5701 gcaatggtgc tgagcaacgt cgactttcca cagccgtttg ccccaaccag aacggtcatt
     5761 ttttctgtgg gaacggagaa agaaacacca tcaacaatga tcttcttgtg atacccggca
     5821 gaaacattgt cgaggatcag cccctgccct tttgcgttat gtgccacgtg tacgccaact
     5881 taatcaaaga caaacaaaaa taaaactcat tctcctttga gagtttgacg ccacaaaatg
     5941 tagtagtcaa gcagttaa
//
LOCUS       DAZKGF010000234         5942 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZKGF010000234 DAZKGF010000000
VERSION     DAZKGF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.234"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            284..1573
                     /gene="sdaC"
                     /locus_tag="ACHOQV_004634"
     CDS             284..1573
                     /gene="sdaC"
                     /locus_tag="ACHOQV_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFP9632894.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1631..2998
                     /gene="sdaB"
                     /locus_tag="ACHOQV_004635"
     CDS             1631..2998
                     /gene="sdaB"
                     /locus_tag="ACHOQV_004635"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFP9632895.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3109..3864
                     /gene="xni"
                     /locus_tag="ACHOQV_004636"
     CDS             3109..3864
                     /gene="xni"
                     /locus_tag="ACHOQV_004636"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFP9632896.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVVGIGPKSATQLLVEFKSLEGIYENLNAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(3919..5067)
                     /gene="fucO"
                     /locus_tag="ACHOQV_004637"
     CDS             complement(3919..5067)
                     /gene="fucO"
                     /locus_tag="ACHOQV_004637"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP9632897.1"
                     /translation="MANRMILNETAWFGRGAVGSLTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPTLAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5095..5742)
                     /gene="fucA"
                     /locus_tag="ACHOQV_004638"
     CDS             complement(5095..5742)
                     /gene="fucA"
                     /locus_tag="ACHOQV_004638"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFP9632898.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         1381 a         1589 c         1473 g         1499 t
ORIGIN      
        1 agtcagatgt cgtttaatgg gcaaatattg cccttaaatt ctcttttact tttgatttac
       61 agagtaaagc gttgggataa tctatcttcc aagtagatta ttgtatttgg gatcaagatc
      121 actgatagat acataacttg tgtgtatctt tccgccctca aattattacg gcggtaaatg
      181 attaagccat cgcctataga cagatttcat ttttacggtc aggcaccttc ccgggctgaa
      241 ctggctaaaa gctgaattat ttgcattcct ccaggagaaa tagatggaaa cgactcaaac
      301 cagcacgatt gcgtcgaaag actctcgtag tgcctggcgc aagacagaca ccatgtggat
      361 gctgggcctt tacggcacgg caatcggcgc gggcgtgctg ttcctgccaa tcaacgccgg
      421 tgttggcggt atgatcccgc tgatcatcat ggctatcctt gcgttcccga tgacattttt
      481 tgctcaccgc ggtctgactc gcttcgtact gtctggtaaa aacccgggcg aagacatcac
      541 cgaggttgta gaagaacact ttggtattgg cgcaggtaaa ctgattaccc tgctctactt
      601 cttcgctatc tacccgattc tgctggttta tagcgtggca atcaccaata cagttgaaag
      661 cttcatgtct caccagctgg gtatgacacc accgccgcgt gcgattctgt cgctgatcct
      721 gatcgtgggt atgatgacca tcgttcgctt cggtgagcag atgatcgtta aagcgatgag
      781 tattctggta ttcccgtttg ttggcgtact gatgctgctg gctctgtacc tgatcccgca
      841 gtggaacggc gcagcactgg aaacgctgtc tctggacact gcatctgcaa ccggaaacgg
      901 tctttggatg accctgtggc tggcaattcc ggtaatggtg ttctcgttca accactctcc
      961 gatcatctct tctttcgccg ttgcgaagcg tgaagagtac ggcgatatgg cagaacagaa
     1021 atgctcgaag atcctggcat tcgcacacat catgatggtg ctgaccgtaa tgttcttcgt
     1081 cttcagctgt gtactgagcc tgactccagc agacctggct gcggctaaag agcagaacat
     1141 ctcgattctg tcttacctgg ctaaccactt taacgcgccg atcatcgcgt ggatggctcc
     1201 gattatcgcg attatcgcta tcaccaaatc cttcctcggc cactacctgg gcgcacgtga
     1261 aggcttcaac ggtatggtga ttaaatctct gcgtggtaaa ggtaagtcta tcgaaatcaa
     1321 caagctgaac cgtatcactg cgctgttcat gctggtaacg acctggattg ttgccaccct
     1381 gaacccgagc atcctgggta tgattgaaac cctgggcggc ccaatcatcg cgatgatcct
     1441 gttcctgatg ccgatgtacg caattcagaa agtaccggca atgcgtaagt acagcggtca
     1501 catcagcaac gtattcgttg tcgtgatggg tctgattgca atctccgcaa tcttctactc
     1561 tctgttcagc taagtccttt cgcgccgctt tcgggcggcg cttcctccgt tttaacgcga
     1621 tgtatttcct atgattagcg tattcgatat tttcaaaatc ggcattggcc cttccagttc
     1681 tcataccgtt ggaccaatga aagcgggtaa acaatttacc gacgatctga ttgcccgtaa
     1741 cctgcttaaa gacgtgaccc gcgtggtggt tgacgtgtac ggctcgctct ctctgaccgg
     1801 taaaggccac cacactgata tcgccattat tatgggcctg gcgggtaacc tgccggatac
     1861 cgtggatatc gattccatcc ctggttttat tcaggatgtg aatactcatg gtcgcctgat
     1921 gctggcaaac ggtcagcatg aagtggagtt cccggttgat cagtgcatga acttccatgc
     1981 cgacaacctt tctctgcatg aaaacggtat gcgcattacc gcactggcgg gcgataaagt
     2041 cgtttacagc cagacttact actctatcgg cggtggcttt atcgttgatg aagaacattt
     2101 tggccagcag gatagcgcac cggttgaagt tccttatccg tacagttcag cagccgatct
     2161 gcaaaaacat tgtcaggaaa ccgggctgtc actctctggc ctgatgatga aaaacgagct
     2221 ggcgctgcac agcaaagaag agctggaaca gcacctggcg aacgtctggg aagtcatgcg
     2281 tggcggtatt gagcgtggta tttctaccga aggcgtgtta cctggcaaac tgcgcgttcc
     2341 acgccgtgct gcggcactac gccggatgct ggtcagccag gataaaacca ccactgaccc
     2401 gatggcggtt gttgactgga tcaacatgtt tgcactggca gtgaacgaag agaatgctgc
     2461 tggcggtcgc gtggtgactg cgccgactaa cggtgcgtgc gggattatcc cggctgtgct
     2521 ggcgtactac gataagttta tccgcgaagt gaacgctaac tcactggctc gttatctgct
     2581 ggtagccagc gccattggtt ctctttataa gatgaacgcg tcgatttctg gtgccgaagt
     2641 gggttgccag ggtgaagttg gcgtggcgtg ctctatggcg gcagctggtc tggcagaact
     2701 gttaggcgca agcccggcgc aggtgtgcat cgcggcggaa atcgccatgg agcataacct
     2761 cggtctgacg tgtgacccgg ttgctggaca ggtgcaggta ccgtgcatcg agcgtaacgc
     2821 cattgcggca gtaaaagcgg tgaacgccgc gcgtatggcg ctgcgccgta ccagcgagcc
     2881 gcgcgtctgc ctcgataaag ttatcgagac catgtacgaa acaggtaaag atatgaacgc
     2941 caagtaccgc gaaacctctc gcggtggcct ggcaatgaag atcgttgcct gcgattaatc
     3001 tctcccggag gcctcgtttt gcgaggcctc ttcccgtttt ctcaaccagc catagcctgt
     3061 tccggcatcg aatgttaccc ttatcgcctg atctttaagg gggttatcgt ggctgttcat
     3121 ttgcttattg tcgatgcact gaatcttatt cgtcgcattc atgccgttca gggttcgccc
     3181 tgtgtcgaaa cctgccagca cgcgctcgat cagctcatta tgcacagcca gccaacccac
     3241 gcggtcgccg tttttgatga tgaaaaccgc agtagcggct ggcgtcatca gcgtttacca
     3301 gattacaaag cgggtcgacc gccaatgccg gaagagttgc acgacgagat gcctgcatta
     3361 cgcgccgcct ttgagcaacg cggcgtcccg tgctggtccg ccagcggcaa cgaagccgat
     3421 gacttagccg ccacgctggc ggtcaaagtg acacaggccg ggcatcaggc aacgattgtt
     3481 tcgacagata aaggctactg tcagttactt tcaccgacat tacgtattcg tgattacttc
     3541 cagaaacgtt ggctggatgc gccatttatc gataaagaat ttggcgttca accgcagcag
     3601 ttgcccgatt actggggact tgcggggatc agcagttcaa aggtaccggg tgttgtgggg
     3661 atcggaccaa aaagcgccac gcagctgctg gtcgagttta agagtctgga agggatatat
     3721 gagaatctga atgcggttgc cgaaaagtgg cgcaaaaaat tagaaaccca taaagagatg
     3781 gcgtttctgt gccgcgatat tgcccgctta caaaccgatt tgcatatcga cggcaattta
     3841 cagcaattgc ggttggtaag gtaacggcgt gccggatacg gcgcaaacgt cgcatccggc
     3901 attatcacat cagcgcattt accaggcggt atggtaaagc tctacaatat cctcaagcgt
     3961 tgcttcacgc gggttgccac cggtacaaac atcatccagt gccgcctgcg ccagtgtcgg
     4021 aatatcttcc ttacgtacac caacatcacg taaatgtggc ggaataccga catcacggtt
     4081 gagagcaaac accgcttcaa cagcggcatt acgcgcctct tccaggctca taccttccac
     4141 tttcacgccc ataacgcgtg cgatatcgcg gtacttctca ccggtaaagt cagcgttata
     4201 acgcataaca tgcggtaaca ggatggcgtt cgcaacaccg tgtggcgtgt tgtaaaacgc
     4261 gcccagtgga tgcgccatac catgcaccaa ccctaaccca acattcgaga atcccatacc
     4321 cgcaacatac tgcccgagcg ccatttcttc tccggcatcc ttatcaccag caaccgatcc
     4381 tcgcagcgcc ccagcaatga tttcaatcgc tttaatgtgc agcgcatcgg ttagcgccca
     4441 cgcgccacgg gtaatatacc cctcaatagc atgagtgagc gcatcgacac ccgtcgcagc
     4501 tttcagcgct ggaggcatac catccatcat gtcagcgtca ataaacgcca cctgcgggat
     4561 atcatgcgga tcaacgcaaa caaacttgcg ccgtttttct tcgtcagtga tcacgtagtt
     4621 aatggtcact tctgccgcag tacctgctgt ggtggggatt gccagaatcg gtacactggg
     4681 tttattggtc ggggaaagcc cttccaggct acgcacatcg gcaaactccg ggttgttgct
     4741 gataatgcca atcgctttac aagtatcctg tggagaaccg ccaccaatag cgatcaggta
     4801 atccgcgccg ctattctgga atacaccgag cccttctttg acgacagtaa ttgttgggtt
     4861 cggcactacg ccgtcgtaaa tcgcccatgc cagccctgca gcatccatct tatcggtcac
     4921 ttttgccacc acgccgcatt gcaccagcgt tttatcggtg acgatcagcg ccttctgata
     4981 accacggcgt ttcacctcat cggttaaaga cccaacagcc ccccgaccaa accatgccgt
     5041 ttcgttcaga atcattctgt tagccatcat ccttctcctt gttgctttac gaaattactc
     5101 ttcaatccgt aacccgtagg ttttgaattt ctccagcact acggcaatct cttcatcgct
     5161 cagcactggc accgggtccg taatcgccag ggtcgtcagg taaagttgtg ccagcacttc
     5221 aacttcatgc gccagccata acgctttctc cagattcgcc tcacaagcga taagcccatg
     5281 atgttgtaac aaagttgcct tacgattttt gagagccagc gcaacatgtt cagaaagttc
     5341 gcgtgttcca aaggtcgcat aaggcgcgca aggaatagaa ttaccgccag ccgccgcaat
     5401 catgtagtga atcgcgggga tcggtcggtt aagaatggaa actgccgtac aatgaacggc
     5461 atgattgtga acaaccgcgt tggcatccgg tctgctttga taggccgcca tatggaaacg
     5521 ccactcgctt gaggggagtt ttccttcctc atgtttgccg ttgccatcaa taaagacaat
     5581 atgcgactcc gtcagttttt catatggaat gcctgtaggc gtaatcagca tcccatcctg
     5641 ataacgtaca ctgacgttac ccgctgtccc ctggttcagt cccaggcggg tcatttccag
     5701 gcaagtgtca ataatctgac gagcaagttt atttcgttcc attagctacc tctctctgat
     5761 tcaaaacagg gcaataatgt tgttcctttc acactattga attagccgtt taattaccca
     5821 ccatcttctt cctgattaac cagaaagaaa ttcacaagct tatattttgt gacctggttc
     5881 aactaatcac agtaaataac tgcaagttct ctttttataa ccctattaaa aatgaccgct
     5941 ct
//
LOCUS       DAZKGF010000235         5919 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZKGF010000235 DAZKGF010000000
VERSION     DAZKGF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.235"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..364
                     /locus_tag="ACHOQV_004639"
                     /pseudo
     CDS             2..364
                     /locus_tag="ACHOQV_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(563..1687)
                     /gene="yhhJ"
                     /locus_tag="ACHOQV_004640"
     CDS             complement(563..1687)
                     /gene="yhhJ"
                     /locus_tag="ACHOQV_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9632899.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(1687..4422)
                     /gene="rbbA"
                     /locus_tag="ACHOQV_004641"
     CDS             complement(1687..4422)
                     /gene="rbbA"
                     /locus_tag="ACHOQV_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFP9632900.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGSVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYHPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     ACLQEAAGQSNEAEAPPVIHDTTHAARQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMVPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(4419..5486)
                     /gene="yhiI"
                     /locus_tag="ACHOQV_004642"
     CDS             complement(4419..5486)
                     /gene="yhiI"
                     /locus_tag="ACHOQV_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9632901.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(5549..5662)
                     /gene="yhiY"
                     /locus_tag="ACHOQV_004643"
     CDS             complement(5549..5662)
                     /gene="yhiY"
                     /locus_tag="ACHOQV_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFP9632902.1"
                     /translation="MMTTLLPVFTKPSPLALNALCAGRICHFLLIPDGRIR"
BASE COUNT         1378 a         1679 c         1530 g         1332 t
ORIGIN      
        1 tatttctatt atccccgatt acagacaagc ctggaaagtg gagcataaat tgtcagatat
       61 tctactgttg actatttgtg ccgttatttc tggtgcagaa ggttgggaag atatagagga
      121 ttttggggaa actcatctcg attttttgaa gcaatatggt gattttgaaa atggtattcc
      181 tgttcacgat accattgcca gagttgtatc ctgtatcagt cctgaaaaat ttcacgagtg
      241 ctttattaac tggatgcgtg actgccattc ttcagatgat aaagacgtca ttgtaattga
      301 tggaaaaacg ctccggcact cttatgacaa gagtcgccgc aggggagcga ttcatgtcat
      361 ttctcgcgtc agtccttgcg gggagcgggc tttcgtaatc ttgccctgaa taccggactg
      421 agtataaaaa agccggttaa ctgaaactgt ccaggttttg gtggtcagtt cataaacgcc
      481 ttatccggcc taaaaaaaac taaaattcaa taaattgcac caatgcgtag accctataag
      541 cttacgcatc gggcaactgt aattacgcca ttgtcccaat tgtcttcctg aatctcagca
      601 gtgcgatggt gaaaaacgcg ccgccaattg ccatcagtgt caggaactgc ggccagacga
      661 tttcgaatcc ggcaccacgg tagagaatgg cctgcgcgag gctaacaaag tgtgtcgtcg
      721 gcatggtcag cataatgtcc tgcaccatct gcggcatact ttcgcgcggc gtggaaccac
      781 cggaaagcat ttgcagcggc agcagcacca gaatcaccag cagccccagt tgcggcattg
      841 aacgcgctat cgtccccata aaaataccga ttgacgtggt ggcaaacaga ctgagcgcca
      901 cgcccagcat aaacagcggg atcgagcctt ctatcggtac gcccagcacg ccttttacca
      961 tcagcaccag cgataatccc gacaccacca gcaccaccag ccccatcgac cagacctttg
     1021 ccatcatgat ctcaaacggc gttatcggca tcaccagtaa gtgttccacc gtgccgtgtt
     1081 cacgctcgcg gatcagcgcc gatccggtca atacaatcgc cagcatggta atgttgttga
     1141 tgatcgccat caccccgcca aaccacgcgg gatcaaggtt cgggttaaag cgcatccgtg
     1201 tttccagcga taccaacggt tcgctgttat cacggtagcg cgcgacaaag ctgttcactt
     1261 caccgttgat aatattctgg atatacccat tgccggtaaa tgcctggctc atgcgcgtgg
     1321 catcgacgtt cacctgaata tccggctggc gtccggcgag gacatcacgc tgaaaattag
     1381 gcggaatatt tatcgcgaag gtataacgtc cggcgtccag cccggcatcc atctcatcgg
     1441 cggtgatcat ctccggtggc aaaaaccacg ggcgatagaa gctgttaacg atccggttcg
     1501 ataactgcga ttgatccata tcggcaatgg cgatcggcgc gaggttcaac gatcctggcg
     1561 taacggtcgc tgacgaatac accgacaccg taaacgagaa gacaatcagc gtcagcatcg
     1621 ctttatcacc gagcagactg cgcaactctt tgatacccag attaaaaata ttacgtaaat
     1681 ggcgcatcat ccctcctgtt ttttcagcag caggatgctt aagcccatca ccagcggtat
     1741 ggctatcagc aacgggataa aaagttgcca caaatcagtc agatccagcg ctttcgagaa
     1801 cgttccgcgg gcgatagtca gaaaatgact ggtcgggtaa acctcgccga tccaacgtcc
     1861 tggaccttcc agcgaagcta ccggatcgat catcccggaa aactgtgtcg ccgggatcaa
     1921 cgtgataatt gccgtaccaa aaatggcggc aatctggctt ttcataaagg tggagatcag
     1981 cagccccatt ccggtggcga tgatgatata cagcagcgcc gccagggtga gagtcaggaa
     2041 actgccttta tgcggtacgc caaatacaaa taccgacagg gcgcagagca ggaaaaagtt
     2101 cagcatcccc agcgcgatgt atggcagctg tttgccaagc aaaaattcgc tacgggtggt
     2161 gggggtcacg taaaggttga taatcgaccc tagctctttt tcccgcacca cgctaagggc
     2221 gcttagcatt gacgggacca tcatcagcag aagcgggatc accgccggaa caatcgccgg
     2281 caggcttttt acgtccgggt tatagcgata gcgcgtctca atattcatca gcccgctttg
     2341 gctggcgggt gtggactgtc ggctcgccac atcctgtagc cagctctggt gcatggcctg
     2401 cacgtagcct tttaccgttt cggcgcggct cggcatcgca ccgtcgatcc agacaccgag
     2461 ttccacgggc gtaccacgcg cgatatcgcg cccgaaatta ggagggatct caattgccac
     2521 cgtgatatcg cccgcacgca tccgacgatc aagctcgtca taactggtga gcggcggctg
     2581 ttcgataaag taacgggaac cggagagatt gagcgtccac gcctggctac tgacggtttg
     2641 atcgcggtca agcaccgcaa agcgcaggtt ttccacatcc atactgatgc cgtaacccat
     2701 tatcagcatc aggatcaccg ttcccatcag cgccagcgtc gaacgtaccg gatcgcgtcg
     2761 cagttccagc gcttcgcggc ggctgtagct aaacagacgg cgcaggctaa atccctgacg
     2821 cgccgcgtgg gtggtgtcgt gtatcacggg cggcgcttcg gcttcgttgc tctgccctgc
     2881 cgcttcctgc aaacaggcga taaatgcctc ttccagactt gcggctccgc gtttctcaac
     2941 cagttcctgc ggtgtcccgc tggcgagcac ttttccggcg tgcatcagtg agatacggtc
     3001 gcaacgttcc gcttcgttca taaagtgggt ggagatgaag atagtcactt tgtcctggcg
     3061 cgagagatcg accatcaact gccagaacat atccctcgcc accggatcga cgccagaagt
     3121 aggctcatcg aggattaaca tctccgggcg atgaatcacc gccaccgcca gcgaaagccg
     3181 ctggcgaatg ccgagcggca atgactccgg cagaacatct tcaacgtcgt tgagcttaaa
     3241 acgctcgctc atttcagcca ctcgcgcggg aatttccgct tccgggatgt gaaacaaacg
     3301 ggcatgtaac tcaaggtttt gccgcacggt gagttcgtta tagagcgaaa acgcctgcga
     3361 catatagcct acccgacggc gggtatcgat atcttttgga tcaaccggtt gcccaaacag
     3421 ccacgcctca ccttcgctgg cgggcagcag tccggtgagc attttcatgg tggtggattt
     3481 accgcagccg ttcgaaccga ggaatccaaa aatctccccg cgtggaatgc ggaaattgac
     3541 gtgatcaacg gcaacgaagg aaccaaaacg catggtcaga tcgcgcgctt cgatggcaat
     3601 ctctgcgttt tcagggtgat acggtgggat cactaccgcc tgatgcgcct ggcgttgcgc
     3661 ttgcggtaac agatttataa atgcttcttc cagcgtggcg ctttgcgttt gctgccgcag
     3721 ctcttcggcg ctgccggttg ccagcacttc tccggcattc atcgctacca gccagtcgaa
     3781 gcgttcggcc tcttccatgt aggcggtggc gaccagcacg ctcatattgc tctgccgctg
     3841 gcgaatgctg tcgatcaaat cccagaactg ggcgcgggag agcgggtcaa cccccgttgt
     3901 tggctcatca agaatcaaca gttccgggtc gtggattaac gcacagcaca gcccaagttt
     3961 ttgcttcatc ccgccggaga gtttccctgc cggacgatca cgaaacggtg ctaacccggt
     4021 gctggtcagc agctcattga ttcgcacttc ccgctccgct ttgtcgtgac cgaacaggcg
     4081 agcgaaaaaa tcgacgtttt catacaccga caaggtgtgg tagaggtttt tgcccagccc
     4141 ctgcggcatc caggcgatgc gcgggcaaac gtcgcggcga tgcttcgggt cgcgcatatc
     4201 gccgcccagc accatcacgc tgccctgctc aatgacgcgg gcaccggaaa tcaacgacaa
     4261 caagctcgat ttaccgacac catccggtcc aatcagcccg accatgcaac gggccggaat
     4321 atcgagtgtg atattgttca gcgcaacggt ttttccataa tgctggctca cgcccgccag
     4381 ttgcgcgaca ggcgggacgg gaaccagttc cagatgcgtc attgcggcaa cctcaccacc
     4441 aggtcgtcag gccacggaag ttcttcattc acccgcaccc acgctacgcc cggcaatccg
     4501 gttttgacat attccagatg ctgctggagt aattccggtg ggatacgcgc tttgacacgg
     4561 aacatcaatt tcagccgctc atcgctggtt tcgacggttt ttggcgtgaa ctgggcgacg
     4621 ctggcgacaa aactgatggt cgcgggaata cgcagatctg gtgcagcatc aagaatcaaa
     4681 cgtgcttcac cgcccagttt cagcgtgccc gcctgttcgg ttggcagaaa gaaggtcata
     4741 tagacgtcgc tgagatcgac catattcagc acccgaccgc ctgccgccag cacttcgcct
     4801 ggctcggcaa cccgatactg cacgcgtccg tcacgcgggg ctttcagttc gctgtcatcg
     4861 atatctgcgg caatgcgccg ttcagtggct tgtgccgctt cgacgcgagt ttgcgcctga
     4921 atgatattgg tgcgtgccgc ctctatagcg gctttagaag cagaaacctg agcttttgcc
     4981 gattccagcg cagctcgggc gctctcagcg gcggcgcggt cgtcatccaa ctgttgcgca
     5041 gaaattgccc cccgttgtgc cagtgagcgg gaacgcgtat gacgttttgc tacggagtcc
     5101 agttctgcct ggcgttgatt aaccagcgac tgtgcggcac gagtttcgct ttggcgttgc
     5161 tccagcaaag cctgcgcggc ggcaacggca ctttgtgctt ctttgatttg cgcgatggct
     5221 tccagtcgct gttcctgcaa cacgcgagta tccatcttcg ccagcacttc accttctcga
     5281 acaaactggc cttctttcac cagaatggtg tcgatacgcc cggcaatttt gctggcgata
     5341 tccacttccg tcgcttcaat gcgcccattg ctgacagcaa agccttccgg cacacccgcc
     5401 gggcgcaaca gccaccacgc cacgacggca gccaccgcca gtgccccgac aacccaccac
     5461 gccagatggc gcttactctt atccataatc gatccgccat aatccctgta agcaatcgac
     5521 gcgtgttaca actgccaccg aacgatgttc agcggattct tccatccggg ataagaagga
     5581 aatggcaaat acgaccagcg cacagagcat tgagcgccaa tggggaaggc ttagtgaaaa
     5641 cgggtagtaa tgttgtcatc atcagcattc ctggccgtaa atgaaacacg catactcata
     5701 agtgaaatca attaaatact aacagttata ttcatgttgt ctatttgatt gccatcaact
     5761 tgtgatacat cgcgatgttc ttccgttcgc tggtgttttt tccaaaaata ttctgtacag
     5821 gttcacagat tctgttgcac aggtttccgt aacagggaaa cctgatgact atcacatagc
     5881 gttattatcg cctgatgtgc gaataaatac cgaatataa
//
LOCUS       DAZKGF010000236         5838 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZKGF010000236 DAZKGF010000000
VERSION     DAZKGF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.236"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            140..370
                     /gene="chaB"
                     /locus_tag="ACHOQV_004644"
     CDS             140..370
                     /gene="chaB"
                     /locus_tag="ACHOQV_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFP9632903.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            528..1223
                     /gene="chaC"
                     /locus_tag="ACHOQV_004645"
     CDS             528..1223
                     /gene="chaC"
                     /locus_tag="ACHOQV_004645"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFP9632904.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGLA"
     gene            complement(1267..1620)
                     /gene="ychN"
                     /locus_tag="ACHOQV_004646"
     CDS             complement(1267..1620)
                     /gene="ychN"
                     /locus_tag="ACHOQV_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFP9632905.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            1806..3200
                     /gene="ychO"
                     /locus_tag="ACHOQV_004647"
                     /gene_synonym="iatA"
     CDS             1806..3200
                     /gene="ychO"
                     /locus_tag="ACHOQV_004647"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFP9632906.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDAFSQQVNQHV
                     ESWLSAWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(3201..3851)
                     /gene="narL"
                     /locus_tag="ACHOQV_004648"
     CDS             complement(3201..3851)
                     /gene="narL"
                     /locus_tag="ACHOQV_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFP9632907.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(3844..5640)
                     /gene="narX"
                     /locus_tag="ACHOQV_004649"
     CDS             complement(3844..5640)
                     /gene="narX"
                     /locus_tag="ACHOQV_004649"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFP9632908.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGIFLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSARFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
BASE COUNT         1435 a         1436 c         1532 g         1435 t
ORIGIN      
        1 cataaaaata agtaatacgt ttagacaatg tttgttttag cacttcttaa gaagagtctg
       61 acatgaaaat tcttatgttt tggcaagagt agatattgtt gaccacactt aatgttcaac
      121 tttgtaaaag gagtcaacga tgccgtataa aacgaaaagc gatctgccgg aaagcgtaaa
      181 gcacgtgctt ccgtctcatg cccaggatat ctataaagaa gcgttcaaca gcgcatggga
      241 tcaatataaa gataaagaag atcggcgtga tgacgccagt cgcgaagaaa cagcgcataa
      301 agtggcctgg gctgctgtga agcatgaata tgccaaaggg gatgatgata aatggcataa
      361 aaaatcgtaa aaccggtagc ttagttaaag ctattcgtgc ggtgttgcct tgcaagtggt
      421 ccgtggattg catattgtcc cgttagtggt ttcaaaatga gcagtaaaaa tgtccggaag
      481 acaccaaaaa gttgtcgcag ggaagtatgc agtggcggag gtgtaaggtg ataacgcgtg
      541 atttcttgat gaatgccgat tgtaaaacgg catttggtgc cattgaagaa tcactcttat
      601 ggtcagcaga acaacgggcg gcttcgctgg cggcgacgct ggcttgtcga cctgatgagg
      661 gaccggtgtg gatcttcggt tatggatcgt tgatgtggaa tccggcactg gagtttaccg
      721 agtcgtgcac cggtacactg gttggatggc atcgtgcgtt ttgcctgcgc ctgaccgccg
      781 ggcgcgggac tgcgcaccag ccgggacgga tgcttgcact gaaagagggc ggacgcacca
      841 caggcgtcgc ctatcgactg ccagaagaga cgctggagca ggaactaacc ctgttgtgga
      901 agcgcgagat gattaccggc tgttatctgc caacctggtg tcagcttgat cttgatgatg
      961 gacgcacagt aaacgccatt gtgtttatta tggacccgcg acatccagaa tatgaatctg
     1021 acactcgcgc tcaggtcatc gcgccgttga ttgcggcggc gagcggtcca ctgggaacca
     1081 acgcacaata cctgttttca ctggaacagg agcttatcaa actgggaatg caggacgatg
     1141 ggctgaatga tttgctggta tcggtaaaaa aactgctggc ggagaattat ccggatggtg
     1201 tgttacggcc gggactcgcc tgagtaaact tccctcatag tggggcgtca gacgccccct
     1261 caaacattaa aatgtgagca ctttatcggc tgacagcgtc cattgcgcca gttccacaag
     1321 cgtaccgatt tccaccccat caatcagagg aagtgtacta atcccgcgcc cgtcggtaca
     1381 ggttttgcac aatttcaccg gtacattctg agcggtaagg atctccagca tttgctgaat
     1441 gttgtagcct tcccctggtt tttgcccgcg caacccggct gtgaccgcat cagacatcag
     1501 gaacagacgc agatccagat tgctctcctg ctctcgtaac gcaatggcca gccgcaagct
     1561 gttaaataag gattcgctcc cgtaaggtgc gccattggca acgatcacga ttttttgcat
     1621 tatttactcc tgtattcagg gaattagaca ctcatcttct atcttactgc ttctgcagcg
     1681 tctgaccaat cggtcacatt tttaaggatt ttcctgaaag cgcgagaaaa tacgacaaaa
     1741 gttgccagta atcgttattc tttagggcta tggtttttca ttttttaccg gaagttaccg
     1801 acgttttgag ccgtttcgtt cctcgcatta ttccgtttta tttactcttg ctggcggcag
     1861 gcggtacagc taacgcacaa tctaccttcg agcaaaaagc ggcaaatccc tttgataata
     1921 acaatgatgg tctgccggat ttaggcatgg cacctgaaaa tcatgatggg gaaaaacact
     1981 ttgctgaaat tgtgaaagat ttcggcgaaa ccagtatgaa tgataacggg ctggatactg
     2041 gcgagcaggc aaaagctttc gcattgggaa aagtccgcga cgcgtttagt caacaggtta
     2101 atcagcacgt agagtcgtgg ctatcagcgt ggggaaatgc cagtgttgac gtcaaagtgg
     2161 ataacgaagg acatttcacc ggcagtcgtg gaagctggtt tgtgccgtta caagataatg
     2221 atcgttatct cacctggagc cagcttggtc ttactcagca ggatgatggg ctggtgagca
     2281 atgtgggcgt tgggcaacgc tgggcgcgcg gcagctggct ggtgggttat aacacttttt
     2341 atgacaactt gctggacgaa aatcttcagc gagcgggctt cggtgccgaa gcgtggggcg
     2401 aatatttgcg actatcggca aacttttatc agccgtttgc tgcatggcat gaacagacag
     2461 ccacgcagga acaacggatg gcgcgcgggt acgacctgac agcccggatg cgcatgccgt
     2521 tttatcaaca cctcaatacc agtgtcagcg tagaacagta ttttggtgat cgtgttgatt
     2581 tgtttaactc tggtacgggt tatcacaatc ccgtcgcgtt gagtctggga ttaaattata
     2641 ccccagtgcc attagtcact gtgacggccc agcataaaca gggtgaaagt ggcgaaaatc
     2701 aaaataacct cgggctgaat cttaattacc gctttggtgt accgctcaaa aaacaacttt
     2761 ctgcgggcga ggttgccgaa agtcagtcgt tacgtggtag tcgctatgac aatccgcagc
     2821 gaaataatct accgactctt gagtaccgac agcgaaaaac gttaacggtg tttctggcga
     2881 caccgccgtg ggatctaaaa cctggcgaaa cagtgccgct gaaattacaa atccgcagtc
     2941 gttacggtat tcggcaactg atttggcagg gcgatacgca gatattaagt ttgacgccgg
     3001 gcgcacaagc caacagtgag gagggctgga cgctgatcat gcctgactgg caaaacgggg
     3061 aaggcgcaag caatcactgg cgattgtcag tggtggtgga agataaccag gggcagcgtg
     3121 tctcctccaa tgagatcacg ctaacgcttg tcgaaccgtt cgacgcattg tcaaacgacg
     3181 aactgcgctg ggaaccgtaa tcagaaaatg cgctcctgat gcacccatac cgcagcttcc
     3241 acgcgagact ttagcttcat tttcttcagc atgtgcttga cgtgaacttt tactgtgctt
     3301 tcggtgatgt ccaggcggcg ggcaatcatc ttgttcggca aaccctgggc aattagcttg
     3361 agaatatcgc gctcgcgtgg ggttaactgg ttaacatcgc gctcagtagt ggcacggtta
     3421 gcgcgcaagc tggcggccag aacaggcgtt aatgcttcgc ttaataccat ttcgccagca
     3481 gctgcctgat gtaacgcttt cagcagatct tccggttcca tatcttttaa cagatagcca
     3541 tccgcgccgc gtttcagtgc ggtgaccaca tcttcttcat ggttagagac gctgaatacc
     3601 acaatgcgcc cggagaggga cttttcgcgc agtttatcca gcgtttccag accgttcatg
     3661 ccgggcatat tgagatctaa caggatcaga tcgggatcaa gagactccgc cagttcaata
     3721 ccctgttcgc cattactcgc ttcgccaacc acggtgatat ctggtgccat actgataagc
     3781 tgttttacgc cagttcgcag catcgggtga tcgtcaatca gcaggatagt agccggttcc
     3841 tgattactca tgggtatctc cttggacgtc tgtgaaagtt ttttcgggaa taaaggtgac
     3901 caccacttcg gtgccacctg attcacgacg gcggacgcgg caatcgcccc gtaaactttg
     3961 cgcacggtcg cgcattatta tcatgccgta gtgattgctg cggatggcat tttcaggcac
     4021 gccgcagccg ttatcctgga cggtcagttt gacctgatta tcgttttgcg ccactgtcac
     4081 cacgacctca ctcgcttgcg aatgtttgag ggcgttactt aatgcctcac gggcaatttg
     4141 caacagatgg attgcctgat gcgaaggcac cagacgaggc ggcaattgat aatccagctt
     4201 caccgggaag ccaaacctgg cgctgtactc ttcgcaactc gcctccagcg ccggacgtaa
     4261 tccaggctcg gtgagctgca agcggaatgt ggtgagcaat tcacgcaact gcgcccagga
     4321 tgcattcagt tcgttacgga tctgacttaa cagttcgcgg ctgctttctg gcagcgcatc
     4381 gccctgcatc tgtaaacaac tcacctgcat cttcatgcaa gagagagatt gggcaataga
     4441 atcatgcagt tcgcgcgcaa tggtggcacg ctcttccatc acgatcaact gttgctgacg
     4501 ttcctgatgg cgatccagcg caagcgtggc ggtgagttgt tcaaccaggg tatccaccag
     4561 ttgttgttga tcatgactaa gatgacgccc ctgcggcagg gttgccagaa aaataccgta
     4621 ctgcgtatga gagtcagcca gccgccactt cagggttgtg ccgcgatcgc caacggggag
     4681 tacaccgcgc gggcagagct gacagccttt atcatcgcag gtcatatctg gctggcaggt
     4741 aaactcctga tgattctctt catcatcagt gtcatacacc cgcagttcga tatcacgtag
     4801 cagggttaaa ttctgtaagc cgttgagtac aggtgacagg cgttcacaca gcggggcgcg
     4861 ggaatgcaaa cggcggttgg cctgccataa aaaagagagg atctgatttt tatgttccag
     4921 cccggcggtt ttctcctgaa cccgctgctc aagtacggcg taactttccg ccagttctgc
     4981 agacatattg ttcaacgcag ttccaagcat agccatttcg ttgcgcccgc tgatgttcgc
     5041 gcgttgggta aaatcgcgat ggctgacggc actcgccatt gccaacaact gccgccacgg
     5101 ttgtagcagt cgcgcccgca accagataat agtgaacacc agtaaaagtg ccataaatac
     5161 cgccattacc cgatggacca gcaccactgt ctcaatgcgc atttccgtgg tgcggtcaaa
     5221 accagatacc agttgatcaa gcccggcaac aaactggctg acatccgctg acaccgtttc
     5281 gcggttttgt gcacgcatta gcgcagggat cagttcatta cgccagtaat cttgtaaacc
     5341 ctgtaattgc gccagttgtc cgtctcgttc tgctgctcgg gttaactcgg cgctaaatgc
     5401 tgtttgttcc atctctttaa ttaagggctt gtctttctcg cttaatggca ctgctgccaa
     5461 cagacggtaa ctttgcatgc gcagcgatcc cgctttgttg atcgcatggg cgctgccctg
     5521 aacgccttgc accagccagc cagaaactgc catccctgcc agtccaatag cagtagaaag
     5581 caacacaata agcgcaacct gattaaccag ggtgagcgga gagagacaac gtttaagcat
     5641 gcaaacctct tccttcaggc tttaaatgag caataacctt aatgaatgtg acgatacatt
     5701 ctggaatggc agtattctcg gctattggct gaagtatacc catacccgga aagagttact
     5761 ccttatttgc cgcgtggtta gtcgctttac atcggtaagg gtagggattt tacagcaccg
     5821 tgaaaaatct cataattt
//
LOCUS       DAZKGF010000237         5771 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZKGF010000237 DAZKGF010000000
VERSION     DAZKGF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.237"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2002)
                     /gene="pqqU"
                     /locus_tag="ACHOQV_004650"
     CDS             complement(<1..2002)
                     /gene="pqqU"
                     /locus_tag="ACHOQV_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFP9632909.1"
                     /translation="MKIFSVRQTVFPALLVLSPTVFAADEQTMIVSAAPQVISELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMSPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEY"
     gene            2244..3305
                     /locus_tag="ACHOQV_004651"
     CDS             2244..3305
                     /locus_tag="ACHOQV_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFP9632910.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3418..4917)
                     /gene="ansP"
                     /locus_tag="ACHOQV_004652"
     CDS             complement(3418..4917)
                     /gene="ansP"
                     /locus_tag="ACHOQV_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFP9632911.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5184..>5771
                     /locus_tag="ACHOQV_004653"
     CDS             5184..>5771
                     /locus_tag="ACHOQV_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFP9632912.1"
                     /translation="MIKVHGVPGWGTTISEMMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKNNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFHDNAQNISSIAEAVCQLPKLQ"
BASE COUNT         1513 a         1485 c         1410 g         1363 t
ORIGIN      
        1 cgtattcttt atcgaataaa ttatcgacac gaccaaataa atccacagtt aaattgtggt
       61 aattgtattt atacccggtg aataagccga cgagagtata agacggcgct ttggccgtat
      121 tttcatcatc tgccataata tcgcccatat aacgcgcttc cgtacctgca taccagccat
      181 cttccggtac ataacctatc gacgcaaagc ccatattacg ggcgatccct ggcatccggt
      241 taccgttaca atcctgttca ttgcaaacat tgctgcgata ggtcgcatca agccaggtcc
      301 aggacgcttt tacgcgaaaa tctcccgcga aacgttgatc ccatgccagt tcagcgcctt
      361 gacgacgggt ctttccggca tttttgtaag tcgtacgccc accgctactg ctatcgacaa
      421 caatttcatc atcggtgtcg gtctggaaca atgcgagact aagcagccca tcaccaatac
      481 gcgttttact gccgatctca attgtatcgt tggtggaggg ttttaaacca aagttcatac
      541 cgctttgccc atcagcgcga taagacagct cattaatcgt cggcgtttca aaaccgcgcc
      601 cggctgccag atagacattc caggcatcgg tcattgcata ttttaacgat ccggctggta
      661 gccatttgtg ataactggca tcaccgctgt cgtcgccgtt acccggagta acgtaatgat
      721 cattggaatc aaaccacaca gagctgtagc gcacgccagc atccagcgaa agtttgtcgg
      781 tcagttgcca ctgggtttgt aaatagggat cgacgttcca catcaaattg cgttcgtcgc
      841 ggcgcaactc acctttttgc ccgtattccg gcacgccgcg attcaggcgg aagttattgt
      901 aacccttgcg gttttcactc atgttttcgt agttcaggcc ggttgtgaac gtgactggaa
      961 cgcccaattc accacggtgt gtccagcggc tgtctatccc ctggtaatgg cgttgcaggg
     1021 taatcacgcc gcccgcatgt gacgggttaa gttgcgggga catggggatt gactggtact
     1081 gggtcgtttc tcgctctccg gcatacatca ttacactcat atcatcttgc gcgctcaggc
     1141 tacgttcata gcgcaaccca gcctgagttt gcttgatggt ttttcgcgtg tcgtactgtt
     1201 ctgcacgggg cgcttgttgt ggattcgcct tccattctgc ttcggttagc ccacctgggt
     1261 catctgcttt gatatccaca ctattgaaaa tcaggcttaa tttgctggcg tcatcaatgc
     1321 gaacgcccag tttggcattg gctaaatttt tctgtgcgcc actatgatca cgatagccgt
     1381 gggtcgtaaa gcgcgtggtt gagacggtgt aatcgacatc gcctggctgt gtgccgtctc
     1441 ccgttgcgcc cgttgctttc agcccatagc gccagctgcc aaaactgccg tagtaactac
     1501 tggcttcaat ggttggtggc tgttgtccgg tctgggtgat gacattcatt acaccgccag
     1561 acgcgttgcc atacagagca gagaaggggc cacgcagcac ttccacattt tgcacactgc
     1621 ttaaatcgat attggatgtc tgcccttgtc cgtcgggcat ggtagcggga ataccgtcaa
     1681 catacaggcg aataccgcga attccgtaag tggagcggga gccaaatccg cgaatcgaca
     1741 gctgtaaatc ttgcgcatag ttctgccggt tttgtacctg caaaccaggc acgccagtca
     1801 gtgattcaga caagttaatg cgcggtgttg ccaggcgcat ctcctcgcca tccaccacgc
     1861 tcacggctgc tggggtatcc agttctgaaa tcacctgcgg cgcggcactg acaatcatgg
     1921 tctgttcatc agcggcaaaa acggtggggg aaaggacaag cagcgcggga aaaacggtct
     1981 gtcggacgga aaaaatcttc atgaaaaaag ccaggttaag aatgggaaaa caccgtcatg
     2041 gtaataaaat tgtaaattta tggaaaacga aacggcacga tatgttaagt aattgagaaa
     2101 attgtagtcg taacggcaag aaatgctcca catttgagaa aataatgatt accattccca
     2161 tttataacaa gagcgtaacg atgattacgc ttagcgaagc attgtgaagc agcaaaaata
     2221 tcggttcatc aaagggagtc gtcatgcatt tacgtcatct gttttcatcg cgcctgcgtg
     2281 gttcattact gttaggttca ttgcttgttg cttcatcatt cagtacgcag gccgcagaag
     2341 aaatgctgcg taaagcggta ggtaaaggtg cctacgaaat ggcttatagc cagcaagaaa
     2401 acgcgctgtg gctcgccact tcgcaaagcc gcaaactgga taaaggcggc gtggtttatc
     2461 gtcttgatcc ggttactctg gaagtgacgc aggcgatcca taacgatctc aagccgtttg
     2521 gtgctaccat caataacacg actcagacgt tgtggtttgg taacactgta aacagcgcgg
     2581 tcacggcgat agatgccaaa acgggcgaag tgaaaggccg tctggtgctg gatgatcgta
     2641 agcgcactga agaggtgcgc ccgctgcaac cgcgtgagct ggtggcagac gatgccacga
     2701 acaccgttta catcagtggt attggtaaag agagcgtgat ttgggtcgtt gatggcgaga
     2761 atatcaaact gaaaaccgcc atccagaaca ccggtaaaat gagtaccggt ttagcgctgg
     2821 atagcaaagg caaacgtctt tacaccacta acgctgacgg cgaattgatt accatcgaca
     2881 ccgccgacaa taaaatcctc agccgtaaaa agctgctgga tgacggcaaa gagcacttct
     2941 ttatcaacat cagccttgat accgcaaggc agcgtgcatt tatcaccgac tctaaagcgg
     3001 cagaagtgtt agtggtcgat acccgtaatg gcaatattct ggcgaaggtt gcggcaccgg
     3061 aatcactggc tgtgctgttt aaccccgcgc gtaatgaagc ctacgtgacg catcgtcagg
     3121 caggtaaagt cagtgtgatt gacgcgaaaa gctataaagt ggtgaaaacg ttcgatacgc
     3181 cgactcatcc gaacagcctg gcactgtctg ccgatggcaa aacgctgtat gtcagtgtga
     3241 aacaaaaatc cactaaacag caggaagcta cccagccgga cgatgtgatt cgtattgcgc
     3301 tgtaatagat atggcgaggg tgaaaatgga gagacttgcc tgacttttat cacgccgtat
     3361 ccggcacctt cgtcagtaat ttgaatttgc ccggcacgta gccgggcaat tttgcaatta
     3421 gctcgccgtt tcaggcttaa acacaatttc ctgtttttct tcatcttcct cgacgactgg
     3481 cgcagtgctg tgaatttcag caacgcgttt gcgcacacca aaccagccta taaccagcag
     3541 aataccgata atcggcagcg ccgcgatagt ataagtcccg ttcgggtaat cgaacgccat
     3601 cagcacaagg acgctcagca aaaacagtaa tgtcagccag gaagtgaagg gcgcgccagg
     3661 cagtttaaaa ctgacatccg ctgctttgcc ttctttaatc gctttacgca ggcgcatctg
     3721 gcacacgatg ataaacgccc atgaagcgat gattcccagc gacgcgaagt tcaacacaat
     3781 ctcaaatacg cgcgacggca ccagatagtt gaggaatacg ccgacgacat acacaaccag
     3841 tgtcgccaga atcccggcat acggcacatg ctgacgactc attttcgcca taaaactcgg
     3901 tgcggaaccg cccatcgcca tcgagcgcag aatacgtccg gtgcagtaca gacctgagtt
     3961 caggctggag agggcagcgg tcagcaccac aatgttcata atgctgccga tgtacggcac
     4021 acccagttta gagaaaaacg tcacgaacgg actttgcccc gcctgatacg cgctccacgg
     4081 caataacata accagcaaca ccacggagcc gacgtaaaac aggccaatac gccaaatcac
     4141 actgttaatc gctttaggca ccatggtctg cggatcttta cattctccgg cagctgtacc
     4201 caccatttca atggaggcaa aagcaaacac tacgccctga atcaacacca gcgcaggtag
     4261 caaaccgtgg gggaagaagc cgccgttatc ggtaattaaa tgaaagccag tggtgttgcc
     4321 atccagcggc tgaccactac cgaggaacac tgtacccacg accaaaaagg tcacaatggc
     4381 gagcacttta ataagcgcaa accagaactc catctcggca aaccatttca caccaatcat
     4441 attcatggtg ccaacgatgg tcagcgcagc gagcgcaaag acccactgcg gtacaccacc
     4501 aaatgcgccc cagtaatgca tatacagtgc aacggcggta atatcgacaa tccccgtcat
     4561 cgcccagttg atgaagtaca tccagcctgc aacataagcg gctttctcac ccaaaaactc
     4621 acgggcataa gagacaaaac tgccactgga agggcggtgt agcaccagct cacccaatgc
     4681 acgcagaata aaaaacgaaa acaagccgca gattaaatag accagtgcca gtgctggccc
     4741 cgccatttgc agtcgggctc ccgcgcctaa aaacaaaccc gtaccaatcg cgccgccaat
     4801 ggcgatcatc tgcacctggc gattgcccat cgctttgtga tacccctctt cgtgggcatt
     4861 aagccagcgg cgtttcgcgg cgtgctgatc tgaagtgtcg gtgtcgtgtt tactcattgc
     4921 tctccctgat tgctttaatg aaaaagtcat ataagttgcc atgaacaatg tttattcatc
     4981 ctggcgatga tagttatcac cattattctt tatgggtata aaactatcgg cgcagcatcc
     5041 tacccgttat cgataaacga tgcaaaacat ccccttacaa ttctgaaggg gattcataca
     5101 actgacgaaa aaatgacaaa tacttttgct ggttaacctg tgtgctgtcc tagacttaat
     5161 ctttaaaaga ttgtgagggg catatgatta aggtgcatgg cgtacccgga tggggcacta
     5221 caatcagtga gatgatgcta acactggctg atattcctta tcagttcgtt gatgtcagtg
     5281 gttttgacca cgagggaact tcacgcgagt tattgaaaac cctgaacccg ttgtgtcaga
     5341 ttccgacact ggcgctggaa aatgacgaga ttatgacgga gacagcggcg attgcattga
     5401 tggttctcga tcgtcgcccg gaccttgcgc caccggttgg gcgcgccgaa cgtcagcagt
     5461 ttcaacggct attggtctgg ctggttgcta atgtctatcc aacattcact ttcgccgatt
     5521 accctgagcg ttgggctcct gacgcacccg aacagttaaa gaataatgtt attgaatatc
     5581 gtaaatcgct ttatatctgg ctgaattcac aattgaccgc tgaaccttat gcgtttggtg
     5641 agcaactaac gctggtggac tgttatcttt gcaccatgcg cacatggggg ccggggcatg
     5701 aatggtttca cgataatgcg cagaatatca gctcgatcgc tgaagctgta tgtcagttac
     5761 cgaaattaca a
//
LOCUS       DAZKGF010000238         5763 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZKGF010000238 DAZKGF010000000
VERSION     DAZKGF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.238"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            272..1435
                     /locus_tag="ACHOQV_004654"
     CDS             272..1435
                     /locus_tag="ACHOQV_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFP9632913.1"
                     /translation="MEARAEATSARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYD
                     SEDILPGIRGEMNLFASGAIEADVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALR
                     EKQSIAVLEQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLK
                     TTLGKLSTWTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKA
                     ALKAQELARYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLD
                     SVARMIDETGRLSLITVRQSRMEMETLRRQEQASARVVDFYRLQFRVARKTLIELLNA
                     ESELYSVGLSRVQTEDQMLHGMLDYLYSQGMLLKWSGVNLSGAEEKVTGQKDR"
     gene            1440..3587
                     /locus_tag="ACHOQV_004655"
     CDS             1440..3587
                     /locus_tag="ACHOQV_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFP9632914.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDMTGAWFWRVLWRFRPYYGQVILA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVLLV
                     ILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNDI
                     VRRPVERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGLSLCIRPGERVGIIGS
                     IGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGLDMQTGEGGQGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRTMVVVTHRLQVLNIVDRIVV
                     MNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            3647..4894
                     /locus_tag="ACHOQV_004656"
     CDS             3647..4894
                     /locus_tag="ACHOQV_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFP9632915.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            5180..>5763
                     /locus_tag="ACHOQV_004657"
     CDS             5180..>5763
                     /locus_tag="ACHOQV_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632916.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQ
                     DGPVLENFTAGQSGTLVEGINNGIIWGGIAFGAGLAAIALAAGGGGGSSRHKRATEDE
                     QKPVQPEGGQEAGKTDVSSTGSIEPESGNLSGRVT"
BASE COUNT         1445 a         1227 c         1658 g         1433 t
ORIGIN      
        1 caataaaatt gaagtatctc acatttttat tatcttctgt cagcgcggaa gataatggcg
       61 gtagcttgga tcaattccgg tgtctctgca tttcaatttt actcaatatt ttacccggtt
      121 gcatcatgat gcctgattat atttcccgcg tgtcccggag gttcgggtga agagaatatt
      181 atctttctgt tttcttttct ctgtccacaa cgtgttcgct tctccactgc cggccctcct
      241 gagggatgct ctcattcatg atccatcagt gatggaggca agggcagaag ccacctctgc
      301 ccgttcccgt gtggagcagg ctcgttcagc acactggccg gtcgtcacag caacaggcag
      361 taaactcctt tcccagagtc accgctacga ctatgactac gactctgaag atattttgcc
      421 cggtatccgc ggtgaaatga atctgtttgc ttcgggagct attgaggcgg atgtcagccg
      481 gagtgagtcg gaagccgagt attaccgtta taaagttgat gaggctggcg aggaaacaat
      541 tcgctccttt gtttcattat atcttgatgc gctaagggaa aagcaatcca ttgcggtact
      601 tgaacagagt ctctcccggc ataacgcaat tcttaatgat cttaacacca tcagtattca
      661 cgataccggg cgggaatctg aatttgttca ggccgaagcc cgcaggctga tggtccggca
      721 gcagataaat acccgcaccc gagtgctcaa aaccacgctg ggaaaacttt cgacctggac
      781 cacaaatccg gtaaccgaag ccgatcttga aaatcctttt tcccggatga cagagtccag
      841 attgttaact gattttacgc agacgcctca gaaagggaat ccgtcgtggc ttgccagtca
      901 ggctgatgta gagagtaaaa aagcggcgct gaaagcgcag gagcttgccc ggtatccccg
      961 ggtggattta acgggatcag tgacccggga tgacaggcag ataggtgtca gtctgtcctg
     1021 ggacctgttc aaccgtaatg ccagttatgg tgtcagtgaa aaggccgcgc agatagctgc
     1081 agctaccgga cgactggact ctgttgcccg aatgattgat gaaaccggac gattatcgct
     1141 gataacggtc agacaaagtc ggatggaaat ggaaacgctg agacgtcagg agcaggcctc
     1201 tgccagagtt gtggactttt atcgtcttca gttcagggtg gcaagaaaga cactgattga
     1261 attactgaat gcagaaagcg aactgtacag tgtcgggctt tcccgggttc agacggagga
     1321 tcagatgctc cacggaatgc tggattatct gtattcccag ggaatgctcc tgaaatggag
     1381 cggagtgaat ctttctggtg cagaagaaaa agtaacaggg cagaaggaca gataaataag
     1441 tgaagagtat ttttgaagga ttatcacagg taacggcatt actgggtaca cctgtatctg
     1501 ctgagacgct ggctgccggt acagtccgga cagatgtctc cgggattgat tttcgttctg
     1561 ttggcgagtt tcttcgcagt gaagggtttg ataatcatct ttcccggcgg gctccggaag
     1621 atattccttc acttgcggtt cctgtgcttc ttttactgaa tgcacaggaa gccattgtgg
     1681 tggtgcgtat tgagggtgcc ggcctggccc ggcagtatca tgtactccag cccgggggac
     1741 tccgtaagac actttctcac gatgaattaa tggctgttta ttcaggttac tgctggtttg
     1801 ttcagccaca ggcaacacag gatcgccgtt cggaactgcc ggagtatgac atgaccgggg
     1861 catggttctg gcgggtactg tggcggttca ggccatatta cggacaggtc attcttgcga
     1921 gttttatcat taactttctt gccctggtca gttccctgta tgtcatgaac gtttatgacc
     1981 gggtcatacc caatcgctcc tgggaaacgt tatggatgct gagtatcggg gtgattgcag
     2041 ccattctgtt tgagtgtgct gcaaagttaa tccggagcta tctgacggat attgcgggta
     2101 agaaggcaga cctgattatc agctcagcac tgttaaggcg ggtgatggca gtgaaaatgt
     2161 cggacaggcc ggcgtcttcc ggttcatacg ccagcaattt gcgggaatat gagtctgtca
     2221 gggagtttat gaccagcgcc acgctgctgg cttttgtgga tttgccgttt atcctgcttt
     2281 tcatcggtgt catcgggctt gtggggggct ggcttgctgt tgttcctctt acactggttc
     2341 cgctggtgat tctggcagga atattactgc agcgaccgct ttcgcgtcat attaatgcct
     2401 ccatgcggga atcatcgcag cgccagggat tacttgtgga ggcactggac ggaatagaaa
     2461 ccctgaagct gaataatgca gttaactggg tccagcgccg ctgggatgga tatacagcca
     2521 gtgtcgcccg ctcgtccata cgggtaaaaa ataccagtaa tgtcataatc tgcttttcca
     2581 cagcagttca gcaactgaat actgttctgc tggtcattct ggggacgtac cttattcaca
     2641 gtgatgaccc acataaccga atcaccatgg gggcactgat tgccgcagtt attctttccg
     2701 gtcgtgcgct ggcaccgctt ggtcagatag catcactggc cacccgtttt cagcaggccc
     2761 gcctggccat gcagggggtg aatgacattg tccgccgtcc ggtggagcgg gaaagtcaga
     2821 cacattatct gacagtgaag gatatccagg gagagtttcg tttcagtgat gtctcctttt
     2881 cttataagcc cggattaccc gacgcattgt gcggtctgtc gctctgtatc cggcccggag
     2941 agcgggtggg tattattggc tcaataggtt gtggtaaatc gacgctgctg aaaatgatgg
     3001 tggggctgta ccgtcagaac aggggacaaa ttactcttga cggggttgcg atgcagcaac
     3061 tggacccctg ttttgtccgg gataaagtgt ttcttctgga acagaatccc cggctttttc
     3121 tgggaactct gcgtgaaaat ctgcagcttt cgcgtcttcc gggaagcctg tcggatgtgg
     3181 atatgattaa tgcagccgga cgtttcgggt ttgaccgttt tatccggcat catccccgcg
     3241 gacttgatat gcagacaggg gaaggcggtc agggactgtc aggaggacag aggcagatgg
     3301 ttgccctgac gagaatggta ctgggttctc cccgggtggt actgcttgat gagcccacga
     3361 caggtctgga cctgcagact gagtctgttg ttctgaaagg tctgagccac tggagccgtg
     3421 gacgcacaat ggtggtggtg acacatcgtc tgcaggttct gaatattgtt gaccgtattg
     3481 tcgtgatgaa tgaagggcgg gtaatgatgg acggtccccg tgatgaaata ctgaaaaaac
     3541 tgtcagggaa acagcagagt ggggagagca gagcgataac ttcatgagcg tcaaccggat
     3601 aagaaaaaag agcagatatg tttaacagtt tgtggaccgc agggatatga cagaaaaaac
     3661 ggaacggaat aaagcgtcgt cagaggtggt caggcagtca gacctggcgc tgttgacaga
     3721 tgtacaggca gcccttcagc aggaaaagca tcatgccctt ttcctgatgg tgatttttct
     3781 gctggtactg gtagtgacgt ttgttatctg ggcatggaac agtcctctgg atgaagtgac
     3841 ccgaggtcag gggagtatta ttcccggcag cagggagcag gttatacaga cccttgaccc
     3901 gggtatactg aaatcacttg aggtcaggga aggcgatatt gttgagaaag gtcaggtact
     3961 gctgacactg gatgataccc ggagctctgc catgctgagg gaaagtgaag cccgggtgaa
     4021 taacctggag gccgtcaggg caagactccg tgcagaagct tattccgaat cactgacatt
     4081 tccggataac gttcctgagg atttgcggga gcgggaaagt acggtctatt ttctccggaa
     4141 gacggagctg gcgcagagca ttgccggtct gaagcagagt aaggcgctgc tggacaggga
     4201 aatagcaatg acgcggccaa ttgtgcgtga aggggcaatg tcggaggtcg aactgttacg
     4261 tatgcagcgt cagtcagcgg agctgcagtt acagatggat gagaaacaga ataaatacct
     4321 gacggaagcc ggtgctgaac tggtgaagac agaagcagag cttgctcagg caaaagagaa
     4381 tatggctggc agagccgacc cggttgagcg ttcacgtctc agggcccccc tgagaggtat
     4441 cgtaaaaaat atacgggtaa acacactggg aggggtggtt tctgcaggac aggacataat
     4501 ggaaattatt ccgctggaag accagttgct gattgaagct tatataaatc cacgggatgt
     4561 tgcctatgtc agaacaggga tgccggcact tgtaaaactg acggcttatg actatgccat
     4621 ttatggtggt cttgacggcg tggtgacact ggtaagtccg gatactttac gtgaccagaa
     4681 acggccggga gatttaaaac tggacccgaa tgaagcttat taccgggtac tggttaccac
     4741 cagtaataat tatctgacag acaggaacgg aaaaagattg ccagttattc ctggcatgat
     4801 agccagcgtg gatataaaaa ccgggcgtaa aacggtattt cagtatctga ttaaaccgat
     4861 aaccagaatg aagcaggcac ttcaggaaag ataataaaaa ggtcttccgg ggaaagtatt
     4921 ttaaatcaat tttaaacggg cagaatgatt tcactttatt ctgcccgggt cattgcgcat
     4981 gcctgtatgt gatttggggc tgaagataat ataaatgtta tatctgtcag gggaaaataa
     5041 atctgaaaaa agcggaatga ttaaggaggt ccggtcagag aaataaatta tctgtaatct
     5101 gattcgtttt tatttaatga ctccctttag cggataaaac ataagagcat actggtttta
     5161 ttaacctgag gatacttata tgaaaatgat tttcactggc aaggtcagtg gagaaaaaac
     5221 agtactgaca gccggagccc gtcatacagt aaaggcacag gccggggaac agtatggact
     5281 ggttgatgag gtgacaggtc tggtcccgga tggtgtggaa gcggaccgtt ctggtgatga
     5341 tttaattctg cgtaaaaagg aagatgatac agaaatccgg attgagggat tctgggaaga
     5401 atgccagccg ggtgaaacac agtgtacagc agtatttaat gttgtgggtg aaaatggtca
     5461 ggtaactgaa gctgtcctga cccaggatgg tcctgtactg gagaatttca ctgcagggca
     5521 aagtggcaca ctggttgaag gtatcaataa cgggattatc tgggggggga ttgcttttgg
     5581 tgcagggctg gcggctattg cactggctgc cggaggcggt ggtggaagca gtcgtcataa
     5641 gcgtgcaact gaagatgagc agaagccggt tcagcctgaa ggcggtcagg aggctggaaa
     5701 aacagatgta tcatccacag gctcaattga gccggaaagt ggaaacctct ctggacgcgt
     5761 gac
//
LOCUS       DAZKGF010000239         5697 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZKGF010000239 DAZKGF010000000
VERSION     DAZKGF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.239"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(132..1046)
                     /locus_tag="ACHOQV_004658"
     CDS             complement(132..1046)
                     /locus_tag="ACHOQV_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9632917.1"
                     /translation="MDKTFSIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGVAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVNVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            complement(1106..1609)
                     /locus_tag="ACHOQV_004659"
     CDS             complement(1106..1609)
                     /locus_tag="ACHOQV_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9632918.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            complement(1622..2152)
                     /locus_tag="ACHOQV_004660"
     CDS             complement(1622..2152)
                     /locus_tag="ACHOQV_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9632919.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            complement(2166..4817)
                     /locus_tag="ACHOQV_004661"
     CDS             complement(2166..4817)
                     /locus_tag="ACHOQV_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9632920.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGHEAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVSDNSACTPLRDRLADASSEFNVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKDADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEEYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTLFIDYFNLFYSKRGQEQISISQQLGHYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTQGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPAVSPETLLNQQTAICR"
     gene            complement(4859..5569)
                     /locus_tag="ACHOQV_004662"
     CDS             complement(4859..5569)
                     /locus_tag="ACHOQV_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9632921.1"
                     /translation="MQTTRPPYSVSFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAASGDISF
                     QTINDYGALTPRIKATMQ"
BASE COUNT         1555 a         1262 c         1244 g         1636 t
ORIGIN      
        1 aatataaaaa ataagactct ggcgttaatt gtgcgcggat tttcttacag gtgtaggata
       61 atagccttca caaaataatt atccgcgcaa ttttcgcgaa tggagcgcat agactaaggt
      121 caaaatcaca attattcata gataaaagtg acaccaatga ctgactgaac tgttccggct
      181 gaaactttgt ttcctgtttg tccataggtt gcgcttaaac ccagaggtac tttgctttta
      241 tttactgtcc ccagggagac gttttctccc gtagccagta tttttccatt acgcatcaga
      301 gagacaccca ctcccgatgc ttttgttgca tctggtgctg tgttagcaaa cacttgtctg
      361 gcactgtcag tggtagcacc cgaaagataa aatgagagct tttgttcact tgagcaatat
      421 acgccaaggg gtatttctgc gcttccagga aagtccggga gattcacgtt gacattacga
      481 ctatcaacgg tgcagccgcc tgttggcata acaacactgt tatttgaaat aatattccac
      541 gtaaaattac gcggatttcc gcttcctaac gtggcaattt tatacatatg aatacgggca
      601 ataacttccc ctgcttttat gacaactcca ccggccactc cgactggggt aatatagagt
      661 tttaacggta atggcatcgg tgtcttatca ccaatatcca atacgttggt atttgtcgtc
      721 aacgggaagg ggtaggtcac attattccag tacagtgatc ctttatatga ctgtagggag
      781 cctgcgaaag ctgatccttg taccaggttt atatgatcag tgtcgtacca gccgccgtaa
      841 tcattccagc aacttatatg ctgagacaag tctacaacca gattctggcc cggttgtatt
      901 acaggatcga gattaacata aacagaggtt gtacctgctc caatacttga cccgccatcg
      961 acattacaac tgaaggcaaa aactttgcct gccattaata gaagataaat accgaacagg
     1021 acctttatac tgaatgtttt atccatgata tatcctaagg ttaaaaaatt gatttaaaaa
     1081 ggaggcaaaa aaaatcaaat tgatgttatt gccaggtata gatcacattg attagcgcct
     1141 cgatcgttcc ctggcttgcg tttccattca ccgtgatagc tcttgcctta agtggaaact
     1201 gtgcattacg agtgatctca tcaacaataa ccgttttgct atcgccattt tttaacgtcg
     1261 catcctggtc atcccttagc tctatctgaa tattttcggc agtaccttca tttttgtaat
     1321 aacccgtatt gtcagttgaa cctgtcacga ttgccgtcac tgcacttgtt tcaaccggac
     1381 aatcggttaa tgacaaagta atattgtgcc agcctgatgc agaaccaggt tgttgcagat
     1441 tgcgcgtata aagatccccg agattaacgt tagcttcttt ggtttgaata gtgcagggtt
     1501 tagcgactac ccgaccatta acagtgatag tgacatcggc ggcctggaga gttgctgtga
     1561 ataacgcgca aaacgtcgct aacaggaacc tcttgtgaag tcttttcata tctggctctg
     1621 attattgaaa ttcaagggta aaggttgccg aagccctcac cagtcccgga ttgacggact
     1681 tctgagttga cttcagacga gcggagtaag gcaaaatatt gttctgttct ggtaccagtg
     1741 ggatccactg catcccggca tgaaggtcat tcaatttcac cggacgcata tttccgtcca
     1801 gtatttctat acccaaaccg gaggccgtgt tgcttccttc atccagtttc aacagagtat
     1861 tattttctgc atcctcaata ccgttaaatg caacccgaac ccccgttgtc cctttactgc
     1921 attcacttaa cgtaatctga aaagggacgg ctggacttgt gctaccggtc gttggaaatt
     1981 gtctggcact gtttttttgg agatctacgg taaaattaag cgaatccgat gagactgtgc
     2041 agccataatc gagaacgcgc ccgctaattt taataacgct atctgcagat aaagcagaat
     2101 aggtggttaa ccccagacat aaaccgagga aaataacgtt attgtatttc ataatctatt
     2161 gttccttagc gacagattgc tgtctgctgg ttcagcaagg tttcaggaga gactgcagga
     2221 agcttgtact cgacaataca gtttgagttt ttatctttgc cccatgaaac ctgtaatttc
     2281 cctgactgtg gaagtccagt cagataaacc tgaccgtttt ccgcgacaat gctgccattt
     2341 ttattctccc catgtgtgac aattgcaccg aatggaacgc tcttattacc gtacttcaac
     2401 gtcattaata ctttcccgcc gatttgtgca ttaaatgttg ctctggcaat agcaccgtga
     2461 gttgggatga cagtgacaac ggtttcatcc agttcaacat tatctgcaag ggaattcgcg
     2521 ttaagagcga cacgattttc tctatattct gtcgcaaatg gtaatatggc atagccacgc
     2581 cagtcggtat gaattccggt ctggttctct attttgacat tatcagcgcc aggagcctta
     2641 accagaacca ttgtgtcgcc cagcggctgt ccaaaggtga tgccatcagc atgagcaata
     2701 atgccaccac tcattccgta ataaatctgg ctgctgtcac cactccgact gtaaccgaca
     2761 ttcgtattac cgtaagctcc acgataatta agagaactgt aaccactggt gccagacgat
     2821 gtattacccc cttgggtgtt accgacctga acgctataat tcaggttatt atccggcagc
     2881 agagtgccat aaacccctga taggttggtc atgccgcctt tcaaatcgtt tgacatactg
     2941 taactggcgt ttgaattacg aaatgccgac tgactgtctg tacgcatcca atgactgaag
     3001 ggaacattaa gcgtaaaagc aagtaaatga tcccgatcgt tttgccatat attattggaa
     3061 tagctgtaat tcagcgaagt cgtaatatca ccaaacggca catttaatcc aaatgatatt
     3121 tgctggtcgc tgcgtgacgt gttccagtaa ctttggcgac tggcactgaa aaatgtcgta
     3181 ccgtaatgtc caagctgctg agagatgctt atttgttcct gaccacgctt actgtaaaac
     3241 agattaaaat aatcaataaa tagtgtctgc tcattgctgt ctccggtagg aggcttgacg
     3301 gtgtaaccac tcattcgact gtaggcactg tcggataagt tataaaaacc ttgcgtagaa
     3361 tagcgatatc ctgcgacctg gatattggtt cccgtctggt agaaggattt gctgtagacg
     3421 gatttaaccg attgtccgct gtgacgggtg tcatctgcaa gcgtcgtatt ggcctgcgtg
     3481 atatcgaaag aaaaggcacc aaaaagtcct aaatctttac caataccaag gttgaaggcc
     3541 tgatattctt ctgcaatttg cattccgcca taaggtgtcc agtttccttc cagtccatgc
     3601 atcaagctac cttgtatgaa tttgggggag ctttgcaggt tatttccact acgatattcc
     3661 cccatggcaa gcgcataacg cgtatatcca gcacgttgga gaaccggaac agacgaataa
     3721 ggaacatata atgtctgaat actgccgtct gcatctttta tggttacttg cagatcacca
     3781 ccattggccg cagagttgat atcatcaata gtaaaagggc cgggtgggac agtagtctga
     3841 taaacatcgt atccattctg ttttacactc acttgtgcgg tgccgcgggc aataccatga
     3901 atcaccggag cgaaaccatg ttggctatcg ggcaacatcg cttctgttga atttattttg
     3961 aggccacgaa agtttacact atcaaaaata tcgccatcgg tataactatc acctaccgtt
     4021 aagcgtgagc gtaagggaat aatgtcacgt tcagccgacg tattgatatg ctgccattta
     4081 ttgctgtcag atgaattgct actcccactg ttataactcc acgttgagtt atcgcgcagt
     4141 cgccaactac caatgttgat gccactctgt aaattcaaat atgcatagtt ggattttcct
     4201 gcattatggt tactacgatt attaatagaa ttaccattaa aactatagtt tagtagccca
     4261 gcatttattc cttcttccca cagatccgga gaaacatagc cgcgagccat tctgccaaca
     4321 taaatttgcg gaacggagag tgatagatgt tgctgaccaa cattaaactc agatgatgcg
     4381 tcagccaaac gatcccgaag tggtgtgcat gcgctgttgt ccgacacatg tttttctgcc
     4441 gaatgttcct tattgtctaa taatgcagat ttttttatgc caagactgac aagaagatct
     4501 gtactcaggc atgggatgag atctgcgtta ttatcatctg caataaaagt aatatcccga
     4561 ctagtcataa attcgtcgtt caaataaatg tcgacccgat atgtaccagg tggtgcctcg
     4621 tgtcccttag taaaggcgga taaatcgaca gattcagcaa gatcattgct taaaaaccgt
     4681 ggattaaaat aatactctgc gtcaacggga aaaaccgcag ttgctaagcc aagggcaatg
     4741 gtaagccgtg caaagcgagg cagtagtaac acctgatgca tacgatatgc cttaaatgcg
     4801 gcacgaaagg ctgtcattat taattccgat tgagtttatt attaattaag tcactgcttt
     4861 actgcatggt tgccttaata cgcggagtta atgcaccgta atcattgatc gtctgaaaac
     4921 tgatatctcc ggatgcggca tgcgggatat ccaccgatac ttcacctttt ggcggcacca
     4981 tggtgtttgg gagattgcta tttcctgctt tcatattcgt tacggtaatg aagtagggcg
     5041 ttggattaat aagagtaagt ttgctaccgc tgcgacgaaa ccgaagcatt gcaggcgctt
     5101 cttcaggtgc cattgccagg ttcgtggggc gataaaacat ttttatgcgg ctaataattg
     5161 ccagctgtaa tgtattctca ttcttctggt ctttttccat agcaggaata gctttaacat
     5221 tcacccagaa caggctttca cggtcgcctg gtaactgatg attagtggcg ttgattatcc
     5281 gcagcgtgtt ctctttttta ccttgcatgg aaaaaagcgg tggcgtaatg acaaactggg
     5341 tgtctttttg gtcacccgca ttttctatcc atgactgaat gagatagaca ctggcaggat
     5401 cattattcgt gacaggcaat aaaacctgtt tctggtttgc cggataaatt acccgcgtcg
     5461 cacccagtgc gacagctgcg ttggcaacgg tgctatggca agcaaagagc aataaaagta
     5521 acactgtcgc cataaaggaa acagagtagg ggggacgcgt agtttgcatc tgatgcattc
     5581 tccatgtgtt aagaggataa gcggggaaac cgttacatca gacaatcggt aaatgttgct
     5641 aatacattgg cgagtcgccg ggtttccaga cttaatgcgt ttgaaataaa tgttggt
//
LOCUS       DAZKGF010000240         5615 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZKGF010000240 DAZKGF010000000
VERSION     DAZKGF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.240"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(36..773)
                     /gene="bamD"
                     /locus_tag="ACHOQV_004663"
     CDS             complement(36..773)
                     /gene="bamD"
                     /locus_tag="ACHOQV_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFP9632922.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQLNAQAEKVAKIIAANSSNT"
     gene            908..1888
                     /gene="rluD"
                     /locus_tag="ACHOQV_004664"
     CDS             908..1888
                     /gene="rluD"
                     /locus_tag="ACHOQV_004664"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFP9632923.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1885..2616
                     /gene="yfiH"
                     /locus_tag="ACHOQV_004665"
     CDS             1885..2616
                     /gene="yfiH"
                     /locus_tag="ACHOQV_004665"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFP9632924.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCSGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYLLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2746..5319
                     /gene="clpB"
                     /locus_tag="ACHOQV_004666"
     CDS             2746..5319
                     /gene="clpB"
                     /locus_tag="ACHOQV_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP9632925.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1393 a         1348 c         1526 g         1348 t
ORIGIN      
        1 gctcaagggc tgccgttttg tgtttcaggt ttctgttatg tattgctgct gtttgcggcg
       61 atgattttcg ctactttttc agcttgcgca ttcaactgca tctgacggta tgcattttcc
      121 atcagcggca gcgcatcacg cgtagcctgg gtatccgggt agtcgcgcaa catgccttct
      181 acgcggttaa cgacggcaac ccatgcacca cgttctgtat agtactcggc aacggagtat
      241 tcatatttcg ccagacgatc tttcaggaat accagacgtt tggtggcatc ggtggtgtac
      301 tgactgttcg gatagccgcg caccagtttg gaaaagtcac taaacgcagc tcgtgcatgt
      361 tgaggatcgc gatcgctacg gtcaacgcca aagaaccctt gcagcgcact gtcatccagc
      421 gccatattgg tcaggccacg catgtacatg acataatcga tattcggatg ggtcgggtta
      481 aggcgaataa aacgatcgat ggcagcctgt gctaacggca aatcggcgtt tttatagtag
      541 gcgtagatga gatccagctg cacctgctgc gaatacggac caaacggata gcgattatct
      601 aacgcttcca gttgcgttat tgcctgtctc cagttaccgt cctgcagctt ttgttgtgca
      661 gtcgcgtaaa tttcatttgg cggattatca ggtacttctt cctttgaccc cgagcaaccc
      721 gccaaaaaca ggcttagtgt ggcggctgcc accagatatt tcatgcgcgt catgacgttt
      781 tgactttcct caaaatgtaa tacgggagat tctctgttcc tgctcccggt taagaccagc
      841 tacaatagca cactatatta aacggcaaag ccgtaaaacc ccaacgataa acgaagaagc
      901 agtatatatg gcacaacgag tacagctcac tgcaacggtg tccgaaaacc aactcggtca
      961 acgcttagat caggctttgg ccgaaatgtt cccggattat tcacgttcgc gaataaaaga
     1021 atggatcctc gaccagcgcg tgctggttaa cggcaaagtt tgtgataagc cgaaagaaaa
     1081 agtattgggt ggcgagcagg tggccatcaa cgctgagatt gaagaagaag cgcgttttga
     1141 accgcaggat atcccgctgg atatcgtcta tgaagatgaa gatatcatcg tcattaataa
     1201 accacgcgac ctggtggtac atcctggcgc gggtaacccg gatggtacgg tactgaatgc
     1261 gttgcttcat tattatccgc ccattgccga tgtaccgcgt gcgggcatcg tccatcgtct
     1321 ggataaagac accaccggcc tgatggttgt ggcaaaaacc gttccggctc agacgcgttt
     1381 agtcgaatct ttgcaacggc gtgaaattac tcgtgagtat gaagcggtgg caattggtca
     1441 tatgaccgct ggcggtacag tagatgaacc aatcagccgc cacccgacca aacgtactca
     1501 catggcggtg catccgatgg gtaaaccggc ggtcactcac tatcgcatca tggaacactt
     1561 ccgtgtgcac acgcgtctgc ggttgcgtct ggaaaccgga cgtacgcacc agatccgcgt
     1621 gcatatggcc catatcactc atccgctggt gggcgatccg gtttatggtg gccgtccgcg
     1681 tccgccaaaa ggtgcttcgg aagcgtttat ctccacgctg cgtaagtttg accgccaggc
     1741 gcttcatgca accatgctgc gtctttatca cccgatctcc ggtatcgaaa tggaatggca
     1801 tgcgcctatt ccagaagata tggtggagct gattgaggtg atgcgcgccg atttcgaaga
     1861 gcataaggat gaagtggact ggttatgagt aagctgattg tcccgcagtg gccgctgcca
     1921 aaaggtgttg cggcctgtag ctccactcgt atcggcggcg tgagcttgcc cccgtatgat
     1981 tcgctcaacc tcggtgctca ttgtggcgat aacccggatc acgttgaaga gaatcgtaag
     2041 cgactttttg ctgcgggcaa tttgccttca aaaccggtct ggcttgagca ggtacacggc
     2101 aaagatgtgc ttaagcttac tggcgaacct tatgcctcaa aacgagcaga tgcctcttat
     2161 agcaacacgc cgggcacggt ttgcgcagtg atgactgccg actgcctccc cgtgctgttt
     2221 tgcaatcgag cgggaacgga agtcgccgcc gctcatgctg gctggcgtgg actgtgctca
     2281 ggcgtgctgg aagagacggt ttcctgtttt gctgataatc cggaaaatat tctcgcctgg
     2341 ttagggccgg caattggtcc acgcgcgttt gaagtggggg cggaggttcg cgaggcgttt
     2401 atggcagtag acgccaaagc gagtacagct ttcattcagc atggtgataa gtatctggcg
     2461 gatatttatc tccttgcccg gcagcgtctg gcgaacgtcg gtgtcgaaca aattttcggc
     2521 ggtgaccgtt gtacatatac ggaaaatgag actttcttct cttatcgtcg cgacaagacc
     2581 accggtcgta tggcaagttt catttggctg atataaccta aagaatcaag acgatccggt
     2641 acgcgtgatt ttcttttcac attaatctgg tcaataacct tgaataattg agggatgacc
     2701 tcatttaatc tccagtagca actttgatcc gttatgggag gagttatgcg tctggatcgt
     2761 cttactaata aattccagct tgctcttgcc gatgcccaat cacttgcact cgggcacgac
     2821 aaccaattca tcgaaccact tcatttaatg agcgccctgc tgaatcagga agggggttcg
     2881 gttagtcctt tattaacatc cgctggcatt aatgctggcc agttgcgcac agatatcaat
     2941 caggcattaa atcgtttacc acaggttgaa ggtactggtg gtgatgtcca gccatcacag
     3001 gatctggtgc gcgttcttaa tctttgcgac aagctggcgc aaaaacgtgg tgataacttt
     3061 atctcgtcag aactgttcgt tctggcggca cttgagtctc gcggcacgct ggccgacatc
     3121 ctgaaagcag caggggcgac caccgccaac attactcaag cgattgaaca aatgcgtgga
     3181 ggtgaaagcg tgaacgatca aggtgctgaa gaccaacgtc aggctttgaa aaaatatacc
     3241 atcgacctta ccgaacgagc cgaacagggc aaactcgatc cggtgattgg tcgtgatgaa
     3301 gaaattcgcc gtaccattca ggtgctgcaa cgtcgtacta aaaataaccc ggtactgatt
     3361 ggtgaacccg gtgtcggtaa aactgccatc gttgaaggtc tggcgcagcg tattatcaac
     3421 ggcgaagtgc cggaagggtt gaaaggccgc cgcgtactgg cgctggatat gggcgcgctg
     3481 gtggctgggg cgaaatatcg cggtgagttt gaagaacgtt taaaaggcgt gcttaacgat
     3541 cttgccaaac aggaaggcaa cgtcatcctg tttatcgacg aattacatac catggtcggt
     3601 gcgggtaaag ccgatggcgc aatggacgcc ggaaacatgc tgaaaccggc gctggcgcgt
     3661 ggtgaattgc actgcgtagg tgccacgacg cttgacgaat atcgccagta catcgaaaaa
     3721 gatgctgcgc tggagcgtcg tttccagaaa gtgtttgttg ccgagccttc tgttgaagac
     3781 accattgcta ttctgcgtgg cttaaaagaa cgttacgaac tgcaccacca tgtgcaaatt
     3841 actgacccgg caattgttgc agcggcgacg ttgtctcatc gctacattgc tgaccgtcag
     3901 ctgccagata aagccatcga cctgatcgat gaggcagcat ccagcattcg tatgcagatt
     3961 gactcaaaac cagaagaact cgaccgactc gatcgccgta tcattcagct caaactggaa
     4021 caacaggcgt taatgaaaga gtctgatgaa gccagtaaaa aacgcctgga tatgctcaac
     4081 gaagaactga gcgacaaaga acgtcagtac tccgagttag aagaagagtg gaaagcagag
     4141 aaagcatcgc tttctggtac acagaccatt aaagcggaac tggaacaggc gaaaatcgct
     4201 attgaacagg ctcgccgtgt gggggacctg gcgcggatgt ctgaactgca atacggcaaa
     4261 atcccggaac tggaaaagca actggaagcc gcaacgcagc tcgaaggcaa aactatgcgt
     4321 ctgttgcgta ataaagtgac cgatgccgaa attgctgaag tgctggcgcg ttggacgggg
     4381 attccggttt ctcgcatgat ggaaagcgag cgcgaaaaac tgctgcgtat ggagcaagaa
     4441 ctgcaccatc gcgttattgg tcagaacgaa gcggttgatg cggtatctaa cgctattcgt
     4501 cgtagccgtg cggggctggc ggatccaaat cgcccgattg gttcattcct gttcctcggc
     4561 ccaaccggtg tggggaaaac tgagctttgt aaggcgctag cgaactttat gtttgatagc
     4621 gacgaggcga tggtccgtat cgatatgtcc gagtttatgg agaaacactc ggtgtctcgt
     4681 ctggttggtg cgcctccggg atatgtcggt tatgaagaag gtggctacct gaccgaagct
     4741 gtgcgtcgtc gtccgtattc cgtcatcctg ctggatgaag tggaaaaagc gcatccggat
     4801 gtcttcaaca ttctgttgca ggtactggat gatgggcgtc tgactgacgg gcaggggaga
     4861 acggtcgact tccgtaatac ggttgtcatt atgacctcta acctcggttc cgatttgatt
     4921 caggaacgct tcggtgaact ggattatgcg cacatgaaag agctggtgct cggtgtggta
     4981 agccataact tccgtccgga attcattaac cgtatcgatg aagtggtggt cttccatccg
     5041 ctgggtgaac agcacattgc gtcgattgcg cagattcagt tgaaacgtct gtacaaacgt
     5101 ctggaagaac gtggttatga aatccacatt tctgacgagg cgctgaaact gctgagcgag
     5161 aacggttacg atccggtcta tggtgcacgt ccgctgaaac gtgctatcca gcagcagatc
     5221 gaaaacccgc tggcacagca aatactgtct ggtgaattgg ttccgggtaa agttattcgc
     5281 ctggaagtta atgaagaccg gattgtcgcc gtccagtaat gataaaacga gcccttcggg
     5341 gctcgttttt gtctataaat tagacggaaa agactatgtt taagatgttt tgcctgaaaa
     5401 atgagcgaac gataaagttt ttatattttt tgcttgtcag gccggaataa ctccctataa
     5461 tgcgccacca ctgacacgga acaacggcaa acacgccgcc gggttagcgg ggttctcctg
     5521 agaattccgg cagagaaagc aaaaacaaat gcttgactct gtagcgggaa agcgtattat
     5581 gcacaccccg cgccgctgag aaaaaagcga agcgg
//
LOCUS       DAZKGF010000241         5588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZKGF010000241 DAZKGF010000000
VERSION     DAZKGF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.241"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            160..416
                     /locus_tag="ACHOQV_004667"
                     /pseudo
     CDS             160..416
                     /locus_tag="ACHOQV_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(438..709)
                     /locus_tag="ACHOQV_004668"
                     /pseudo
     CDS             complement(438..709)
                     /locus_tag="ACHOQV_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206187.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(706..2466)
                     /locus_tag="ACHOQV_004669"
     CDS             complement(706..2466)
                     /locus_tag="ACHOQV_004669"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFP9632926.1"
                     /translation="MTNSRIPDERQFLTSDNTELFYRHWPAQEASSTPKVVVLFHRGH
                     EHSGRLQHIVDELAMPDTAFYAMDARGHGRSPGLRGYSPSLVRSVQDVDEFVRFAAAD
                     SQAGMTDVVVVAQSVGAVLAATWAHDYAPDIRGLVLASPAFKVKLYVPFARQGLELMY
                     RLRGLFFVNSYVKGKYLTHDPERVASFNKDPRITRPIAVNILLDLYKTAERVVSDASA
                     ITLPVQLLVSGEDYVVHRQPQLDFYQNLRNPQKELHVLPGFYHDTLGEKERHLAFDRM
                     RSFIDTLYARPVQRFDYSQEDRWSPSADTWRILSGGPALYSLDDISYRCLRYGMKVLG
                     TQSTGVRLGFETGFDSGSTLDYIYRNQPQGSTSLGRLIDKNYLNSVGWKGIRQRKIHL
                     QMLIQQAVARLTEQGSSVRIVDIAAGHGRYVLDALQGETAVSEILLRDYSELNVAKGQ
                     EMIASRGMAGRARFEQGDAFNRNELAALEPRPTLGIVSGLYELFPENGPVRDSLAGLA
                     AAIEPGGILIYTCQPWHPQLKTIAWSLTSHKNGKAWVMRVRTQGEMDALVRDAGFEKC
                     TQLIDEMGIFTVSMAVRRQP"
     gene            complement(2459..3100)
                     /locus_tag="ACHOQV_004670"
     CDS             complement(2459..3100)
                     /locus_tag="ACHOQV_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP9632927.1"
                     /translation="MMTRLLRTLFTLCIVWPVIWLWLGLRVRHRERLPKHGPSIIVAN
                     HNSHMDVFALLSLFPLRYQASVHPVAAADYFLRDKRMAWFALKILNIIPISRKGGYTD
                     PLAQCEQALREGKTLILFPEGTRGEPGVLAPLKSGVWHLSQRMPEVPIIPVGLRGTER
                     VMAKGNRIPLPLFIDVNIGDALSTHPEKKTFMDDLEQRLTALLQPSQGNHPHD"
     gene            complement(3104..4021)
                     /locus_tag="ACHOQV_004671"
     CDS             complement(3104..4021)
                     /locus_tag="ACHOQV_004671"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9632928.1"
                     /translation="MTLLEKSLIVIFSLLLIATVINGLLVWLRPSKDWRELTLRIRTW
                     WVIIVLFSFALLSPSWLALTFFALVSFMALKEFLTLVPSRQSDRMPLLWMFIAIPLNY
                     WLIGIGWYGMFVVFIPVYVFLFLPARMVIAGDTQGFLRTVSQLHWSLMTTVFAFSHVA
                     FLLVLPADGMQTSALLVLFLVGLTEFNDIAQYLWGKSLGRIKVIPKVSPNKTLEGLLG
                     GVATTALAATVLGPLLTPLSWPMALLAGVIIGVTGFCGDVVMSAIKRDIGVKDSGTLL
                     PGHGGILDRLDSLIFTAPVFFHFVRYFCY"
     gene            complement(4018..4623)
                     /locus_tag="ACHOQV_004672"
     CDS             complement(4018..4623)
                     /locus_tag="ACHOQV_004672"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9632929.1"
                     /translation="MTLYQIKPSFQALLRPLMFWLNRRGVTANHITLCAMVLSLITGV
                     VLIIHPQPGLFVLLPVVLFVRMALNALDGMLARECNQRSRLGAILNETGDVISDISLY
                     LPFMLLPQSSIPLVLIVVFCALMTEFCGVLAQTINGVRSYAGPLGKSDRALVFGAWGL
                     SLSIWPQIVAWNNVIWGVATLLLLWTIFNRCRSALREEGVK"
     gene            complement(4681..4866)
                     /locus_tag="ACHOQV_004673"
     CDS             complement(4681..4866)
                     /locus_tag="ACHOQV_004673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632930.1"
                     /translation="MLTQLHRVLYRCPGHYVYGSATQSTGTGTGSLASRNTSQSPLPA
                     QQAGFPRPELYSLSVCQ"
     gene            complement(5107..>5588)
                     /locus_tag="ACHOQV_004674"
     CDS             complement(5107..>5588)
                     /locus_tag="ACHOQV_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9632931.1"
                     /translation="MAGSIMVALPDINDRIDNITGYILIVAGVLSASFYVVISSRLIV
                     NFEPVTLLTGQLTFSFILACFFIVLTGTSLQLPIESVFLVFLSGILQYFLAFCFYLYS
                     LRWIPVHIAGAMLYFIPLVALLLSWFFLQEDISFIQGLGVFITILCVYILNKKYAIE"
BASE COUNT         1534 a         1477 c         1359 g         1218 t
ORIGIN      
        1 tgttgtggtt ctgacagaag taaattaatt tgaagtaaat aaagcaaaat atcagaagaa
       61 tatatcgggg cgcacagtct atctctgcct ttgcttcatg ggatgttctt tgtatatttt
      121 acttgctgtt taagtatgta tataaggagt gcattcatta tgccaatgaa gcttgtaaag
      181 aagaatcccc ggagcaaagt gattcgggag cttagctgtg agcaagagat ggcagtaaag
      241 gcggtctgcg gactgaaaaa acagcaacta cggcactaca tgccttggcc aatgatcttc
      301 tccgggaaat gcgtgagtat gatgtatggt tacagtgtga ctgtattccg ggagattcgc
      361 cgaccatgaa ttttgctgct cttaaaaaca acacgggaac tttttatctg tccagtttgg
      421 atatagataa ttattgtact aataagggaa gccaacacca gccgacagac cagacgccgc
      481 tgctcattca gtgaaacgca gataaacagt aaagcaaatc cagactccag tacggaataa
      541 tcgtcagcgg tattaacggg atccggtgct cccagtcaaa caccaggctg ctgatgtttt
      601 ctctcccggc ggtaaactga ttcgtcatgc cataagtaag aaagaagaat ggggccagca
      661 ccattagcca gccgattccc tgaataatca ccgcacgccg cgttgtcatg gttgacgtcg
      721 taccgccata gagaccgtga agatgcccat ttcatcaatt agttgcgtgc atttctcaaa
      781 accggcgtca cggaccagcg cgtccatctc gccctgagta cgtacgcgca ttacccaggc
      841 tttgccattt ttgtgactgg tgagtgacca ggcgatggtt tttaactgag ggtgccaggg
      901 ctgacaggtg tagatcagaa tgcctcccgg ttcaatcgct gcagccagcc cggcaagcga
      961 atctcgtacc ggcccgtttt ccgggaacag ttcatacaga ccggacacga tccccagcgt
     1021 cggacgcggt tccagtgccg ccagttcatt acgattaaag gcatccccct gctcaaatcg
     1081 cgcacggccc gccataccgc gagaggctat catctcctga cctttcgcca cgtttaattc
     1141 gctgtaatcg cgcagaagga tctcgcttac cgccgtttcg ccttgcaggg catcaagcac
     1201 atagcgtcca tgcccggcgg caatatccac gatccggacc gagctaccct gctctgttaa
     1261 ccgagcaaca gcctgctgaa ttaacatctg cagatgaatt ttacgctggc ggatcccctt
     1321 ccagcccacg ctattcaggt agtttttatc aatgaggcgt cccaacgagg tgctgccctg
     1381 aggctggttg cgatagatgt agtctagggt actgccggaa tcgaaaccag tctcaaatcc
     1441 cagacgcacc cccgtggact gcgtgcccag cactttcatc ccgtagcgca aacagcggta
     1501 agagatatcg tccagcgaat acagcgcagg gccaccgctc agtatgcgcc aggtatcggc
     1561 gctggggctc cagcgatctt cctggctgta atcaaaacgc tgcacgggac gggcatacaa
     1621 ggtatcaata aaactgcgca tcctgtcaaa tgccagatgc cgctcttttt cgcctaaggt
     1681 gtcatgataa aatcccggca gtacatgcag ttctttctgc ggatttcgca ggttctgata
     1741 aaaatcaagc tgcggctggc ggtgcacaac gtaatcttcc ccggagacca gcaactgcac
     1801 aggtagcgta atggcgctgg catcgctgac gactcgctca gccgttttgt agagatcaag
     1861 cagaatattc acagcaattg gtcgggtaat ccgcggatcc ttattgaagc ttgctacccg
     1921 ctcgggatcg tgcgtcagat acttgccttt cacatacgag ttgacgaaga aaagaccgcg
     1981 caggcgatac atcaattcca gcccctggcg agcaaacggc acatacaatt tgaccttaaa
     2041 cgccggtgat gccaacacca ggccacgaat gtccggggca taatcatgcg cccaggttgc
     2101 cgccagaacc gccccgacgc tttgcgccac gacgaccaca tccgtcatcc cagcttggct
     2161 atctgcggcg gcgaaacgca caaactcgtc aacatcctgc accgaacgca cgagcgacgg
     2221 actgtaacca cgcagacccg gtgagcgacc atgacctcgg gcatccatgg cataaaacgc
     2281 tgtatccggc atcgccagct catcaacaat gtgttgcaaa cgcccggagt gctcatgccc
     2341 acgatgaaac aggaccacca cttttggcgt tgaactggct tcttgtgctg gccagtggcg
     2401 ataaaacagc tctgtattat cactggtcag aaactgccgt tcatcgggta tgcgggaatt
     2461 agtcatgggg atgatttcct tgcgagggtt gcaaaagcgc cgttagtctt tgctccaggt
     2521 cgtccataaa cgttttcttc tccggatgcg tacttagcgc atcgccaata tttacatcaa
     2581 taaacagcgg caaagggata cgattacctt tcgccatcac acgctcagta ccgcgcaacc
     2641 caacggggat aatgggcacc tccggcatgc gctgactcaa atgccacact ccggatttca
     2701 atggcgcaag tacgcctggc tcaccgcgcg tcccttccgg aaacaggatc agtgttttac
     2761 cttcacgcag cgcctgttca cattgcgcca gcggatcggt ataaccgccc tttcttgaaa
     2821 taggaatgat gttaaggatc ttcagcgcaa accaagccat gcgtttatcc cgcagaaaat
     2881 aatccgcggc agcaaccgga tgaacagaag cctggtaacg cagcggaaaa agcgaaagca
     2941 aggcgaaaac atccatatgg ctgttgtgat ttgccacgat tatcgaaggc ccgtgcttcg
     3001 gcagtcgctc gcggtggcgg acacgcagac caagccacag ccagatgacc ggccagacga
     3061 tacacagcgt aaacagggta cggagtagtc gtgtcatcat aggttagtag cagaaatagc
     3121 gaacaaaatg gaagaagacc ggtgcggtaa aaatcagcga atccagccga tcaaggatgc
     3181 ctccatggcc gggtagcagc gtaccactgt ctttgacgcc gatatcacgc ttgattgccg
     3241 acatcaccac gtcgccgcaa aagccagtaa cgccgataat aaccccggct aacagtgcca
     3301 tcggccagct caggggagtc aacagtggcc ccaggactgt tgccgccagc gccgtcgttg
     3361 ccaccccacc cagcaatccc tccagggttt tgtttgggct gaccttcggt atgactttta
     3421 tacgaccaag cgacttgccc cacaggtatt gcgcgatgtc gttaaattcg gtcaggccga
     3481 ccaaaaacag caccaacagc gcgctggttt gcattccatc cgccggtaac accagtaaaa
     3541 acgcgacatg gctgaaggca aatacggtgg tcatcagact ccagtgcaac tgggaaacgg
     3601 tacgtaaaaa gccctgtgta tccccagcaa tcaccattcg cgccggtaaa aaaaggaaca
     3661 cgtagaccgg gataaacacc acaaacatgc cgtaccagcc gatacctatc agccagtagt
     3721 taaggggaat tgcaataaac atccataaca gcggcatgcg gtcggactgg cgggaaggca
     3781 ccagcgtcaa aaactctttt agcgccataa agctgaccag cgcgaagaac gtcagtgcca
     3841 gccagctagg acttaatagt gcgaaggaaa acaggacgat aatcacccac caggtgcgaa
     3901 tacgtaatgt cagctcgcgc cagtctttac ttggacgcag ccacaccagc aacccgttga
     3961 ttacggtggc aatcaacagc agggaaaaaa taacgatcag tgatttttca agtagcgtca
     4021 tttcacaccc tcttcacgca atgcgctgcg acagcgatta aaaatcgtcc ataacaacaa
     4081 cagtgttgcg acaccccata tgacattatt ccaggcgaca atctgcggcc agatcgacag
     4141 tgacagcccc catgcaccaa acaccagcgc tctgtcgctt ttgcccagcg gaccggcata
     4201 gctgcgcacg ccgttaatcg tttgcgccag cacgccgcaa aactcggtca tcagcgcaca
     4261 aaacaccacg atcagcacca gcggtatact gctctgcggg agtagcataa agggcagata
     4321 aagcgagatg tcggaaatta catcacccgt ctcattgagg atggccccca gacgcgatct
     4381 ttgattacat tcacgcgcca gcataccatc gagcgcattg agcgccattc gtacaaacaa
     4441 caccacgggc agaaggacga ataatccggg ctgaggatga ataatgagca ccacgccggt
     4501 gataagggaa agcaccatcg cgcacagtgt aatgtgatta gcggtgacgc cgcgcctgtt
     4561 cagccagaac atcagcggtc ttaacagagc ctgaaacgag ggctttatct ggtaaagggt
     4621 catcatcact ccttaataag catatttgac ggggttgagt ctggcctttt attggggctg
     4681 tcactggcac actgaaagtg aatacagttc cgggcgcgga aatcctgcct gctgcgccgg
     4741 taaaggcgac tgactggtat tacgggaggc cagagagcca gtacctgtgc cggtagactg
     4801 tgtggctgaa ccataaacat agtgtcctgg acaacggtac agcactctgt gcagctgtgt
     4861 cagcatgcgc ttttctttaa ttatgcccag tcatgtgcgg aggcaaggat ttcaaaatat
     4921 ccgttacatt tgttgcatga tgccttatcg ctggtccggg ctattgcttt acttctttta
     4981 aatcagctct gatgaacatt ttttaccact ttatcgcatt aaggttatgt cattcatcgt
     5041 ctggcatcac gtttttggtt atggtgtcag ggtgtaccct accgcgataa cgcaacccta
     5101 ataaagtcat tcgatggcat atttcttatt gagaatatat acgcataaaa tagttataaa
     5161 aacgcctagt ccctgaataa atgatatatc ttcctgcagg aagaaccagg acaacagcaa
     5221 tgctaccaga ggaataaaat agagcattgc tcctgctatg tgaacgggaa tccagcgtag
     5281 ggaataaaga tagaaacaaa aagccaaaaa atattgtaaa attcctgaca aaaacacaag
     5341 aaatacagat tctattggca gttgcaggga tgtgccagtt agaacaataa aaaaacaagc
     5401 aagaataaaa gagaaagtca actgcccggt taacaacgta acaggctcaa aattcacaat
     5461 aagtcgtgat gagataacaa cataaaagga tgctgagagt acacctgcaa caataagtat
     5521 atatcctgta atattatcaa tgcggtcatt gatatcaggt agggcaacca ttatactgcc
     5581 tgccattg
//
LOCUS       DAZKGF010000242         5442 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZKGF010000242 DAZKGF010000000
VERSION     DAZKGF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.242"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(180..1553)
                     /gene="cysG"
                     /locus_tag="ACHOQV_004675"
     CDS             complement(180..1553)
                     /gene="cysG"
                     /locus_tag="ACHOQV_004675"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFP9632932.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGQVVGLRDK
                     LNWFSNH"
     gene            complement(1572..2378)
                     /gene="nirC"
                     /locus_tag="ACHOQV_004676"
     CDS             complement(1572..2378)
                     /gene="nirC"
                     /locus_tag="ACHOQV_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFP9632933.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSIRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            complement(2505..2831)
                     /gene="nirD"
                     /locus_tag="ACHOQV_004677"
     CDS             complement(2505..2831)
                     /gene="nirD"
                     /locus_tag="ACHOQV_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFP9632934.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2828..5371)
                     /gene="nirB"
                     /locus_tag="ACHOQV_004678"
     CDS             complement(2828..5371)
                     /gene="nirB"
                     /locus_tag="ACHOQV_004678"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFP9632935.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHANLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
BASE COUNT         1281 a         1493 c         1391 g         1277 t
ORIGIN      
        1 gaacgataca ggaatattta atcggcgtca gtgagtgaat gacttaattt tgtaagtcgc
       61 tgtaacgttg aatataaccc attagcagat aatatttatt aaggggagtg tttttatctg
      121 ctgaatatat aaagagagtt gtaaacgaaa aaggccagaa ttctctggcc ttggttaaat
      181 taatggttgg agaaccagtt caatttatcg cgcaggccaa caacctgacc aataataatt
      241 agcgatggac tgttcatttg ctgcgccagt tcgcccagtt gtgtgagcgt accgtcaatc
      301 acgcgctgcg tgactgctgt tccgttttcg acaattgcca ccggcatttc gccaggcata
      361 ccgtgttcaa tcagcttttg ctgaatagtc gcggcctgat tcaaccccat atagaacacc
      421 agcgtctgtt tttctgccgc caggttttcc cagtccagtt cgccaccggt ttttaagtgt
      481 ccggtaatta agcgtacgct ctgggcataa tcgcgatgcg tgagcggaat acccgaatag
      541 gcagagcaac cagaagctgc ggtaataccc ggaaccaccg agaacggaat acccgcgttg
      601 cacagtgttt ccagctcttc gccaccacgg ccaaaaataa acggatcgcc gcctttcagc
      661 cgcaccacgc gtttgccttt ttgcgcttcc cgtagcagga tctggtttat ctcttcctgc
      721 ggtacgcagt ggtatcccgc gcgtttgccg acgaaaacgc gatcagcatc gcggcgtacc
      781 agattcataa tatcgtcaga aaccagacgg tcgtagacca ccacatctgc ctgctgaatt
      841 tgttgcagac ctttcagtgt cagcagcccg gcatcgcccg gacctgcacc aaccagcacc
      901 acttcaccgc gatggtcgag cggttcgttg attaactgtt cggtggtttc agtaatggct
      961 ttctgatcgt tgtttgccag cgactgcgcc aggcggtcgt taacgaacaa tttctcccag
     1021 aaacggcgac gctcacccat cgtggcgaac tgttgtttca ctcgcccgcg taattgcccg
     1081 gcgtatttcg ctacctggcc cagatgcagc ggcagcagtg attcgagttt ttcgcgcaac
     1141 aggcgcgcca gaaccggaga ggtgccgcca gaggagaccg cgaccattag cggtgagcgg
     1201 tcaataattg acggcataat aaagctggcg gctttcggcg catcgaccac gttacagaag
     1261 atgcgacgag cttcagcggc ttcactgacg cgctggttaa gcgcgtcatc atccgtcgca
     1321 gcaatcgcca gccagcaggt gtcgagaagg ctttcatcaa atggcccttc gacgagggtt
     1381 aacatgcctg catctgccca tgcggtgaac tgtggaataa acgctaatgc attcaccgtt
     1441 aagcgagcgc ctgcgtctaa cagtaacctt gctttgcgtt ccgcgacatc accaccgccg
     1501 acaatcagac agtcgcgatc gcgtaattgg caaaatatag gcaaatgatc cacgtaaaaa
     1561 ccccttagta attaaccggc agctgtttca gtttgattaa atttgtccgc aaccggacga
     1621 ttcgctttcg gcgtagcata ccaataaccc aatcccatga atacggcacc tgataaagta
     1681 ttacccagcg tcacccacag caggttatga ccaatacccg ccagcgtgta ggcttcgctg
     1741 tggttgccga accaggagag ggcgaacagc gtcatgttag cgatggagtg ctcgtagccg
     1801 gacgcgataa atgccagcag acaccaccag atagcgataa atttcgccgc cccttcagtg
     1861 cgcagcgcca tccagatagc caggcaaacc agccagttac acaatgcacc tttgaagaac
     1921 agtaccattg ccggtgcagt ggtttttgcc agcgcgacgg agtgaacgat gctggtatct
     1981 accggcagca ggctaccgcc gccccagcta tagagcatgg caacgaagac ggaaccgacc
     2041 agattaccca gccaggtttg cggcaggatt gcccacattt gcccgtggct gatggtgccc
     2101 gctttcaccc caaaggtgag gaacatggtg tgtccggtga acagttcaga accggcgata
     2161 atcaccagcg ttaaggcgat accaaaggtc gcgcccatca ccagaggacg tatggacgga
     2221 tcgagcaaat taccgagcgt gaaaatcagg atgatcccaa gacccacata cgcgcccgcc
     2281 atggcggagc tgacccaaaa gccgagcggg ttatttgccg acaggcgtgc aatgcgcgca
     2341 gcgttagccg cacacttatt aatagtgtct gtaaacattt gattatcctt taaaaaataa
     2401 aaacaaaaaa ataaaacaaa aagtttaaaa aatgtcctgc aatataccga aggtcattac
     2461 aggaccatgc aaaaagggga ggcattgcgc ctcccgttaa aacattaacc gcgcagctgc
     2521 accacgccgt ctttcactcg tgcttcgtaa tgtttgacgg aaaactgttc gtcttccatg
     2581 cacaagccat cacttaagcg aaaacgctgt tttttcagcg ggctggcgac ccacagctcg
     2641 ccctggtgtt ccgcaatcag cccgcgtgac agcacgctgg actcgaagaa cggatcgatg
     2701 ttgctgatcg caaacacctg atcgctgtga tacgggcgga aaattgctac ttgttcgtca
     2761 cctaacagcg cgcagacgcc ggtttcaggc aggatgtcat cgattttgca gatgtctttc
     2821 cactggctca tgcgttgtcc tccaccagag ttaccgggat acgttcatac ggcgttgccg
     2881 gacggtgctg ttcgcgctct ggcaccatct gcacgttcgg gtcacgctta tcgctgttga
     2941 tgaagtgttt gaagcgagtc tgcgcagacg gggtattgac cgtttcagtc cactcacaca
     3001 ctaccgcttc acgcaggcgc gccatctctt cttccagatg tgcgttcagc cccagtttgt
     3061 cgtcaatgat caccgctttc aggtaatcga tgccaccttc gaggttttct aaccacggcg
     3121 cggtacgcgt cagtttgtcg gcggtacgga tgtagaacat catgaagcgg tcgagatatt
     3181 tgatcagcgt ttcgcgatcg atatccgctg ccaacagatt cgcatgacgc ggtttcatgc
     3241 cgccgttacc gcaaacatac aggttccagc ctttttcggt ggcgataata cccacgtctt
     3301 taccctgagc ttctgaacat tcacgggtac agccggagac accgaacttc attttgtgcg
     3361 gagtccggat acctttgtag cggttttcca gttccacgcc gaggccgacg ctgtcgccca
     3421 cgccgtagcg gcaccaggtg ctacccacgc aggttttcgc catacgcagt gctttcgcat
     3481 aggcatgacc ggtttcgaag ccggcttcaa tcagctgacg ccagatctcc ggcagatcgt
     3541 ctttctgtgc gccaaacatc gccaggcgct gggagccggt gattttggtg tagagattaa
     3601 attcacgcgc gatacgacct actgccatca gcccttccgg ggtgatttca ccgcccggag
     3661 aacgcgggat caccgagtag gtgccgtctt tctggatgtt agcgaggaag ttatcgttag
     3721 aatcctgcag cggagtatgt tccggtttca gaatgtattc gttccagcag gaggccagca
     3781 gcgaaccgac ggtcggttta caaacttcac aaccgtagcc tttgccgtgt ttcgccagca
     3841 gttcttcgaa ggttttaatg ccttcaacgc ggatcaaatg gaacagttcc tgacgcgaat
     3901 aagcaaagtg ttcgcacagg ttgttgttaa cttcgatgcc ctgtttcgcc agttccgcgt
     3961 tcagtacctg agtgaccagc gggatacagc caccgcagcc agtacccgct ttggtttcag
     4021 ctttcagcgc cgcaacggtg tggcagcctt tgttgatggc agcaatcaga tcacctttgg
     4081 tgacgtcgaa gcaggagcag atttgcgcgc tgtccggcag tttatcaaca ccgatagacg
     4141 gcttaccgct acccgagtgc gccggcagaa tcagagaatc cgggttttcc ggcagctcga
     4201 tagcattcag caccagttgc agcaggttgc cgtagtcgct ggtatcgccc accagtaccg
     4261 caccgagcag ggttttgttg tcttcgctga caataaggcg tttgtagatc tctttgcttt
     4321 cgtcgaggta aacgtagcta cgtgcgccag gtgtgcgacc gtgcgcatca ccaataccgc
     4381 ctacgtctac gcccagcagt ttcagcttgg cgctaaggtc tgcaccttca aaggcgtttt
     4441 cgctaccaag aatatggtca acggcgacct gcgccatttt gtagcctggt gctaccagac
     4501 caaatacacg gttgttccag cttgcgcatt caccgatggc gtagatatcc ggatcggaag
     4561 tctggcagga atcatttatg acgatacccc cacgcggagc aacgtccaga ccacactggg
     4621 ttgccagctt atcgcgcgga cggataccgg tagagaagac gataaagtcg acttccagtt
     4681 cgctgccgtc ggcaaaacgc atggttttac gcgcttcaac accttcctgc acaatctcaa
     4741 gagtgttttt gctggtgtga acgcgcacgc ccatactttc gattttgcga cgcagctgct
     4801 cgccgcccat ctgatcaagc tgttccgcca tcagcatagg ggcaaattcg ataacgtggg
     4861 tttcaatgcc taagtttttc agcgcgccag cagcttccag acctaacagg ccgccaccaa
     4921 caacggcacc gcgcttgctg cgacgagcgc aggattcaat ggcgttgagg tcttcaatgg
     4981 tgcgatagac aaagcagtcc tgagtatcag aacctttaat tggcgggatc cacgggtagg
     5041 aaccggtcgc catgatcagc ttgtcataaa aaacggtacg tccggcgctg gagtgaatca
     5101 ccttctcctg acggttgatg gtgatagcgc gttcgccgac cagaactttg atgccgtgtt
     5161 tctcgtagaa gccttcgcgc accagcgaca gctcttcggc ggtgtggtga gagaagtaag
     5221 acgagaggtg tacgcggtca taagcgatgc gcggttcttc acagaaaacg gtaatatcaa
     5281 agttggccgc atcagattta tcaagaagat cttcgataaa gcgatggccg accataccgt
     5341 taccgataat tgcgagtctg actttgctca tttttgcctc gatttctttt ctattaccgc
     5401 ctaccttaac gattcagcaa ccccgcttat tgatgtaaat ca
//
LOCUS       DAZKGF010000243         5423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZKGF010000243 DAZKGF010000000
VERSION     DAZKGF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.243"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            97..1431
                     /gene="garP"
                     /locus_tag="ACHOQV_004679"
     CDS             97..1431
                     /gene="garP"
                     /locus_tag="ACHOQV_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFP9632936.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1447..2217
                     /gene="garL"
                     /locus_tag="ACHOQV_004680"
     CDS             1447..2217
                     /gene="garL"
                     /locus_tag="ACHOQV_004680"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFP9632937.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2247..3137
                     /gene="garR"
                     /locus_tag="ACHOQV_004681"
     CDS             2247..3137
                     /gene="garR"
                     /locus_tag="ACHOQV_004681"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP9632938.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3234..4379
                     /gene="garK"
                     /locus_tag="ACHOQV_004682"
     CDS             3234..4379
                     /gene="garK"
                     /locus_tag="ACHOQV_004682"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFP9632939.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4412..4788
                     /gene="rnpB"
                     /locus_tag="ACHOQV_004683"
     ncRNA           4412..4788
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHOQV_004683"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(4997..5098)
                     /locus_tag="ACHOQV_004684"
                     /pseudo
     CDS             complement(4997..5098)
                     /locus_tag="ACHOQV_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5354..>5423)
                     /locus_tag="ACHOQV_004685"
     CDS             complement(5354..>5423)
                     /locus_tag="ACHOQV_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFP9632940.1"
                     /translation="QLINVNFFSSDRTSFCECNRFP"
BASE COUNT         1297 a         1270 c         1509 g         1347 t
ORIGIN      
        1 ttcccgagtt cattgtcgta acggcataaa tgaaactcat aaataacacc ttccccataa
       61 taataaaaat cagcataagt acccgaggta aataaaatga tgctggacac cgttgacgaa
      121 aaaaagaaag gcgtgcatac ccgctattta atattactga ttatttttat tgttaccgcc
      181 gttaactacg ccgatcgtgc aacgctgtct attgctggta ccgaagtggc aaaagagttg
      241 cagttaagtg cggtttcgat gggttacatc ttctctgctt ttggctgggc ctacttgctg
      301 atgcaaatcc ccggcggctg gctgcttgat aagtttggtt cgaaaaaagt ttacacctac
      361 agcctctttt tctggtcgct attcaccttc ctgcaaggct ttgttgatat gttcccgctg
      421 gcctgggcag ggatctccat gttctttatg cgctttatgc tggggttctc ggaagcgcca
      481 tcattcccgg cgaacgcccg tattgtcgcc gcctggttcc cgacgaaaga acgtggtact
      541 gcctccgcca tctttaactc ggcgcaatat ttctcgctgg cgctcttttc gccgctgctt
      601 ggctggctga ctttcgcgtg gggctgggag cacgtcttta ccgttatggg ggtaattggt
      661 tttgtgctga cggcgctgtg gatcaagttg attcataacc cgacagatca cccacgtatg
      721 tctgcggaag agctgaagtt tatctcggaa aatggcgcgg tggtcgatat ggaccacaaa
      781 aagccgggca gtgcggcagc aagcggaccc aaactgcatt acatcaagca attgctctct
      841 aaccgcatga tgctgggcgt atttttcgga caatatttta tcaacaccat cacctggttc
      901 ttcctcacct ggttcccaat ttatctggtg caggaaaaag gcatgtcgat tctgaaagtg
      961 ggtctggtcg cctcgattcc ggcactgtgt ggctttgcgg gcggcgtgct gggaggtgtc
     1021 ttctcggatt atctgatcaa acgcggtttg tccctgaccc tggcacgtaa gctaccaatt
     1081 gtgctgggaa tgttgctggc ttccaccatc atcttatgta actacaccaa caacaccacg
     1141 ctggtggtca tgctgatggc gctggctttc tttggtaaag gatttggtgc gctgggctgg
     1201 ccagtgattt ctgacaccgc gccgaaagag attgttggcc tctgcggcgg cgtctttaac
     1261 gtctttggca atgttgcctc cattgtcact ccactggtga ttggctacct ggtaagtgaa
     1321 ctgcactcct tcaatgcagc actggttttt gtgggatgtt cagcgctgat ggcgatggtc
     1381 tgctacctct tcgtagtcgg cgacattaaa cgtatggaat tgcagaaata agcaaaggta
     1441 taagcgatga ataacgatgt tttcccgaat aaattcaaag ccgcactggc tgcgaaacag
     1501 gtacaaattg gttgctggtc agctctctct aacccgatta gcactgaagt tcttggtttg
     1561 gctgggtttg actggctggt gctggatggt gaacatgcgc caaacgatat ttccacgttt
     1621 attccgcagt taatggccct gaaaggcagc gccagcgcgc cagtagtgcg agtgccgacc
     1681 aacgagccgg taattattaa gcgtcttctg gatatcggtt tctataactt cctgattcct
     1741 tttgtagaaa caaaagagga agcagagcag gcggtggcat caactcgtta cccaccggaa
     1801 ggcattcgcg gcgtctccgt ttctcaccgc gccaatatgt ttggcaccgt ggcggattat
     1861 ttcgctcagt cgaacaagaa catcactatt ctggtccaga tagaaagtca gcagggcgta
     1921 gataacgtcg atgctattgc cgctaccgaa ggtgtagacg gcatcttcgt cggccccagc
     1981 gatctggctg cggcattagg ccatctcggc aatgcctcgc acccggatgt acaaaaagca
     2041 attcagcaca tttttaaccg tgccagcgcg cacggcaaac ccagcggcat cctcgcacca
     2101 gtcgaggctg atgcgcgtcg ttatctggaa tggggcgcga cgtttgtggc cgtcggcagc
     2161 gatctcggcg tcttccgctc tgccactcag aaactggctg atacctttaa aaaataacca
     2221 ccaccgcaat aagagagatg attgatatga ctatgaaagt tggttttatt ggccttggga
     2281 ttatgggtaa accaatgagt aaaaaccttt tgaaagcagg ttactcgctg gtggttgctg
     2341 accgtaaccc agaagctatc gctgacgtga ttgctgcagg tgcagaaaca gcctctacgg
     2401 ctaaagcgat cgctgaacag tgcgacgtaa tcataaccat gctgccaaac tcccctcatg
     2461 tgaaagaggt ggcgctgggt gagaatggca ttattgaagg cgcgaagcca ggtacggtat
     2521 tgatcgatat gagttctatc gcaccgctgg caagccgtga aatcagcgaa gcgctgaaag
     2581 cgaaaggcat tgatatgctg gatgctccgg tgagcggcgg tgaaccgaaa gccatcgacg
     2641 gtacgctgtc agtgatggtg ggcggcgaca aggctatttt cgacaaatac tatgatttga
     2701 tgaaagcgat ggcgggttcc gtggtgcata ccggggaaat cggtgcaggt aacgtcacca
     2761 aactggcaaa tcaggtcatt gtggcgctga atattgctgc gatgtcagaa gcgttaacgt
     2821 tggcaaccaa agcgggcgtt aacccggatc tggtgtatca ggcaattcgc ggtgggctgg
     2881 cgggcagtac cgtgctggat gccaaagcgc cgatggtgat ggaccgcaac ttcaaaccgg
     2941 gcttccgtat tgatctgcat attaaggatc tggcgaatgc gctggatact tctcacggcg
     3001 tcggcgcgca actgccgctc acagctgcgg ttatggagat gatgcaggca ctgcgtgcag
     3061 atggtttagg aacggcggat catagcgccc tggcgtgcta ctacgaaaaa ctggcgaaag
     3121 tcgaagttac tcgctaatga cgtcgtgccc ggatggcgta ttgcaatccg ggcctggaat
     3181 ccaggccgaa taagagaaac gccctccggc gtcatgtggt aacaggcata ggtatgaaaa
     3241 tcgtaatcgc cccagactct tataaagaaa gtttatctgc cagcgaggtt gcgcaggcga
     3301 tagaaaaagg atttcgggaa atttttcctg atgcacagta tgtttctgtt ccggttgccg
     3361 acggtggtga aggaacggtg gaagcgatga ttgcagccac ccagggggct gaacgtcacg
     3421 cctgggttac agggccgctg ggcgagaaag tgaatgccag ttgggggatc tccggcgatg
     3481 gcaaaaccgc gtttatcgaa atggcggcgg ccagtgggct ggaactggta cctgcggaaa
     3541 aacgcgatcc actcgtgacc acttcacgcg gtactggtga gttaatcctg caggcgctgg
     3601 agagcggtgc gacaaacatt attatcggca ttggcggcag cgccaccaac gacggcggcg
     3661 caggcatggt acaggcgctg ggggcgaaat tatgcgatgc caacggcaat gaaattggtt
     3721 ttggcggcgg tagtcttaat actctgaatg atattgatat ttccggtctc gatccgcgct
     3781 taaaagattg tgtcatccgc gtcgcttgtg atgtcaccaa cccgttagtc ggcgacagtg
     3841 gtgcttcgcg catctttggc ccacaaaagg gagccagtga ggcgatgatt gtcgagctgg
     3901 acaataacct ctctcactat gccgatgtta ttaaaaaagc gctgcatgtt gatgtgaaag
     3961 atgtccccgg cgcaggagct gcgggcggta tgggcgcggc gctgatggcg tttcttggtg
     4021 cagaactgaa aagtggtatt gaaatcgtca ctacggcgct gaatctggag gaacatattc
     4081 acgattgtac gctggtgatc actggtgaag ggcgtattga tagccagagt attcacggca
     4141 aggtaccgat tggtgtcgca aacgtggcga agaagtacca taaaccggtg attggcattg
     4201 cgggtagcct gaccaatgat gttggcgttg tacatcagca tggcattgat gcggtcttca
     4261 gcgtattgac cagcattggt acgttggacg aagcattccg cggggcttat gacaatatct
     4321 gccgtgcttc acgtaatatc gccgcgacac tggcgattgg aatgcgcaac gcggggtgac
     4381 aagggcgcgc aaaccctcta tactgcgcgc cgaagctgac cagacagtcg ccgcttcgtc
     4441 gtcgtcctct tcgggggaga cgggcggagg ggaggaaagt ccgggctcca tagggcaggg
     4501 tgccaggtaa cgcctggggg ggaaacccac gaccagtgca acagagagca aaccgccgat
     4561 ggcccgcgta agcgggatca ggtaagggtg aaagggtgcg gtaagagcgc accgcgcggc
     4621 tggtaacagt ccgtggcacg gtaaactcca cccggagcaa ggccaaatag gggttcataa
     4681 ggtacggccc gtactgaacc cgggtaggct gcttgagcca gtgagcgatt gctggcctag
     4741 atgaatgact gtccacgaca gaacccggct tatcggtcag tttcacctga tttacgtaaa
     4801 aacccgcttc ggcgggtttt tgcttttggt gcccagggta agatgaatat ctacggtatt
     4861 aataatagac tttgtaagaa gttattaagt ctatattgca tttaaaaggt cgtaattata
     4921 catatatttc tctcattaat tgtaagttcg caggtattgt ccgatcgttg aatcatttgg
     4981 acgttataac ctaaattttt accgtccaat aatcaatatt tctgttgtga ttgttaacca
     5041 ctgcgtgcat tggcgggatt gaagggtgaa aaatatgtgc aattgggaag cctttcattg
     5101 atgcctgcct tgtcatcatg acaaacaata atagatgctt attttcctcg cagggttctt
     5161 tcgcactatc aaaaaatgct catttacccg gcattacagt caacgaagca acattctatg
     5221 gcctacgctg atgtaaagca cagtgagaat cccccttgtt gtcttcgtct gaccttatcg
     5281 cggcgaccag caattattcg ttttgtgatc ctcaacacaa aatgctattc gtcgcctatg
     5341 aggggagagg aatttatgga aaacgattgc attcacaaaa tgacgtcctg tcagaagaga
     5401 aaaagttgac gttaatcaat tgt
//
LOCUS       DAZKGF010000244         5418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZKGF010000244 DAZKGF010000000
VERSION     DAZKGF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.244"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            163..1221
                     /gene="citC"
                     /locus_tag="ACHOQV_004686"
     CDS             163..1221
                     /gene="citC"
                     /locus_tag="ACHOQV_004686"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFP9632941.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFCHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1236..1532
                     /gene="citD"
                     /locus_tag="ACHOQV_004687"
     CDS             1236..1532
                     /gene="citD"
                     /locus_tag="ACHOQV_004687"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFP9632942.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1529..2437
                     /gene="citE"
                     /locus_tag="ACHOQV_004688"
     CDS             1529..2437
                     /gene="citE"
                     /locus_tag="ACHOQV_004688"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFP9632943.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2448..3980
                     /gene="citF"
                     /locus_tag="ACHOQV_004689"
     CDS             2448..3980
                     /gene="citF"
                     /locus_tag="ACHOQV_004689"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFP9632944.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVYLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3984..4535
                     /gene="citX"
                     /locus_tag="ACHOQV_004690"
     CDS             3984..4535
                     /gene="citX"
                     /locus_tag="ACHOQV_004690"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFP9632945.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKLGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4510..5388
                     /gene="citG"
                     /locus_tag="ACHOQV_004691"
     CDS             4510..5388
                     /gene="citG"
                     /locus_tag="ACHOQV_004691"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFP9632946.1"
                     /translation="MSMPATSTKNTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
BASE COUNT         1237 a         1524 c         1436 g         1221 t
ORIGIN      
        1 tcacgctgca atactcctta ggatgtatag cgaaaggaga aaaagatata cctcgatcgc
       61 ccccctttct cccaagtgaa aataaaaggt tatcagtttg caacatcgaa caacatttgt
      121 tgcaaatcga taacaacatg caccttcagg atactattta ttatgttcgg caatgatatt
      181 ttcacccgtg taaaacgttc agaaaataaa aaaatggcgg aaatcgccca attcctgcat
      241 gaaaatgatt tgagcgttga caccacagtc gaagtattta ttaccgtaac ccgcgatgaa
      301 aagcttatcg cgtgcggtgg aattgccgga aatattatta aatgcgttgc tatcagtgaa
      361 tccgttcgcg gtgaaggact ggcgctgaca ttagccaccg agctaattaa cctcgcctat
      421 gagcggcaca gcacgcatct atttatttat accaaaaccg aatacgaggc gctgttccgc
      481 cagtgcggtt tttccacgct gaccagcgta cccggcgtga tggtgctgat ggaaaacagc
      541 gccacgcgac tgaaacgcta tgccgaatcg ctgaaaaaat tttgtcatcc agggaacaag
      601 attggctgca ttgtgatgaa cgccaatccc tttacgaatg gtcaccgtta tctgattcaa
      661 caggctgcgg cacagtgcga ctggttgcat ctgtttttag tcaaagaaga ttcttcacgc
      721 ttcccctatg aagaccgact ggatctggtg ttaaaaggca ccgccgatat tccacgcctg
      781 actgtgcatc gcggctccga atacatcatc tcccgcgcta cgttcccttg ctacttcatt
      841 aaagaacaga gcgtcattaa ccattgttac accgaaattg atctgaagat tttccgtcag
      901 tacctcgctc ccgcgctggg tgtaactcac cgctttgtcg gtactgaacc cttttgtcgc
      961 gttactgccc agtacaacca ggatatgcgc tactggctgg aaacaccgac tatctccgca
     1021 ccgcccatcg aactggttga aattgagcgg ctgcgttacc aggagatgcc gatatccgct
     1081 tcccgggtac gtcaactgct ggcgaaaaac gatctcacgg ctatcgcgcc gctggtccct
     1141 gcagtcacgc tgcattattt gcagaacctg cttgagcact cccgccagga cgcggcagct
     1201 cgtcaaaaga cccccgcatg agaaacaggt gaaaaatgaa aataaaccag cccgccgttg
     1261 caggcaccct tgagtctggg gatgtgatga tacgcatcgc cccactcgat acgcaggata
     1321 tcgacctgca aatcaacagc agcgttgaga aacagtttgg cgatgcaatt cgcaccacca
     1381 ttctggacgt tctcgcccgc tacaacgtgc gcggcgtaca gctgaatgtc gatgacaaag
     1441 gcgcactgga ctgcatttta cgtgcacgac tggaagccct gctggcacgc gccagcggta
     1501 tcccggctct gccatgggag gattgccaat gatttccgct tcgctgcaac aacgtaaaac
     1561 tcgcacccgc cgcagtatgt tgtttgtgcc tggtgctaat gccgcgatgg tcagcaactc
     1621 ctttatctac ccggctgatg ccctgatgtt tgacctcgaa gactccgtag cattgcgtga
     1681 aaaagacacc gcccgccgca tggtttacca cgcgctgcaa catccgcttt atcgcgatat
     1741 tgaaaccatt gtgcgtgtaa acgcgctgga ttccgaatgg ggtgttaacg acctggaagc
     1801 tgtcgttcgc ggtggtgcgg acgttgtgcg tctgccgaaa accgataccg ctcaggatgt
     1861 tctggatatc gaaaaagaga tcctgcgtat cgaaaaagcc tgtggtcgtg aacccggcag
     1921 taccggcctg ctggcggcga ttgaatctcc gctgggcatt acccgcgcgg tagaaatcgc
     1981 tcacgcttcc gagcgtttga tcggtatcgc cctcggtgca gaagactatg tgcgcaacct
     2041 gcgtacagaa cgctccccgg aaggaactga actgctgttc gctcgctgct ccattttgca
     2101 ggccgcgcgc tctgcgggta ttcaggcgtt cgataccgtc tattccgacg ctaacaacga
     2161 agccggattc ctgcaagaag ccgcccacat caaacagctg ggctttgacg gcaaatcgct
     2221 gatcaacccg cgtcagattg atctgctgca caacctctac gcaccgaccc agaaagaagt
     2281 ggatcacgcc cgccgcgtcg tagaagccgc tgaagccgcc gctcgcgaag gcctcggcgt
     2341 ggtttccctg aacggcaaga tggtggacgg tccggttatc gatcgcgccc gtctggtgct
     2401 ctcccgtgca gaactttccg gcatccgcga agaataaggc aatcaaaatg acgcagaaaa
     2461 ttgaacaatc tcaacgacaa gaacgggtag cggcctggaa tcgtcgcgct gaatgcgatc
     2521 ttgccgcttt ccagaactcg ccaaagcaaa cctaccaggc tgaaaaagcg cgcgatcgca
     2581 aactgtgcgc caacctggaa gaagcgattc gtcgctctgg tttacaggac ggcatgacgg
     2641 tttccttcca tcacgctttc cgtggcggtg acctgaccgt caatatggtg atggacgtca
     2701 tcgcgaagat gggctttaaa aacctgactc tggcctccag ctccctgagt gattgccatg
     2761 cgccgctggt agaacacatt cgccagggcg tggttacccg catttatacc tctggcctgc
     2821 gcggtccact ggcggaagag atctcccgtg gtctgctggc ggaaccggtg caaatccact
     2881 ctcacggcgg acgtgtgcat ctggtacaga gcggcgaact gaatatcgat gtggctttcc
     2941 tcggcgtccc gtcctgtgat gaattcggta atgccaacgg ctactccggt aaagcctgct
     3001 gcggctccct cggctatgca atggttgatg ccgacaacgc aaaacaggtc gtgatgctta
     3061 ccgaagaact gctgccttat ccgcataatc cggcaagcat tgagcaagat caggttgatc
     3121 tgatcgtcaa agttgatcgc gttggcgatg ctgcaaaaat cggcgctggt gctacccgta
     3181 tgaccactaa cccgcgcgaa ctgcttattg cccgtagcgc tgcggatgtg attgttaact
     3241 ctggctactt caaagaaggt ttctccatgc agaccggcac cggcggcgct tcgctggcgg
     3301 taacccgttt cctggaagac aaaatgcgta gccgcgatat tcgcgccgac ttcgccctcg
     3361 gcggtattac cgcaacgatg gtttatctgc acgaaaaagg tttgatccgc aaactgctgg
     3421 atgtgcagag ctttgacagc catgctgcgc aatcgctggc ccgtaacccc aatcacatcg
     3481 aaatcagcgc caaccagtac gctaactggg ggtcgaaagg cgcagcggtt gatcgtctcg
     3541 acgtggtggt actgagcgcg ctggaaattg acacccagtt caacgttaac gtgctgaccg
     3601 gctctgacgg cgtactgcgt ggtgcttccg gtggtcactg cgataccgcg attgcttctg
     3661 cgctttccat catcgtcgcg ccgctggtac gcggtcgtat tccgacgctg gtggataacg
     3721 tgctgacctg catcaccccg ggctccagtg tcgatattct ggtcacagac cacggcatcg
     3781 ctgttaaccc ggcacgtccg gagctggcag aacgtctgca ggaagcgggc attaaagtgg
     3841 tttccattga gtggctgcgt gaacgtgcgc gtctgctgac cggtgaacca cagccgattg
     3901 aattcaccga ccgcgtcgtt gccgttgtgc gttaccgcga tggctcggtg atcgatgttg
     3961 tgcatcaggt gaaggaataa gccatgcacc tgcttcctga actcgccagc caccatgcgg
     4021 tgtcaattcc cgagctgctc gtcagccggg atgaaaggca agcacggcaa cacgtctggc
     4081 tcaagcgcca tcctgttcca ctggtctcct ttaccgtggt tgcgcctggg ccgattaaag
     4141 acagcgaggt cacacgccga atttttaatc atggcgtgac agccttgcgt gccttagccg
     4201 caaaactggg ctggcaaatt caggagcagg ctgcactggt ttccgccagc gggccggagg
     4261 gcatgttgag cattgccgcc ccggctcgcg acctcaagct cgccaccatt gagcttgaac
     4321 atagtcatcc tctcgggcgg ttatgggata tcgatgtcct gacacccgaa ggcgaaattc
     4381 tctcccgccg cgactattca ctgccgcctc gccgctgcct gttgtgcgag caaagcgcag
     4441 ctgtttgcgc gcgtggaaaa acccatcaac tgaccgattt actcaaccgc atggaggcgc
     4501 tgctgaacga tgtcgatgcc tgcaacgtca actaaaaaca caaagcttgc gacgtcatta
     4561 atcgatgagt acgccctgct gggctggcgc gccatgctga ctgaagtcaa tctgtcaccg
     4621 aaaccaggcc tcgtggatcg cattaactgc ggtgcgcaca aagatatggc gctggaagat
     4681 ttccaccgca gcgcgctggc gattcagggc tggttacccc gtttcattga atttggtgcc
     4741 tgtagtgcag aaatggcacc ggaagcggta ctcaacggat tacgcccaat tggtatggct
     4801 tgcgaaggtg atatgttccg cgccactgcg ggcgtaaaca cgcataaagg aagcattttt
     4861 tctttagggc tactgtgtgc ggcaattggt cgtttgcttc agctcaacca gccggtaacg
     4921 ccaacaaccg tttgttctac ggcggcaagt ttctgccgtg gcctgaccga tcgcgaactg
     4981 cgtaccaata attcacgact gacggcaggt caacggttgt accagcagct tggccttacc
     5041 ggcgcacgcg gtgaagccga agcgggttat ccactggtga tcaaccacgc cttgccgcat
     5101 tacctcactc tgctggatca ggggttagat cctgagctgg cattgctcga taccttgctc
     5161 ctactgatgg ctaccaacgg cgataccaac gttgcatcgc gcggtggcga ggggggcctg
     5221 cgctggctac agcgcgaggc gcaaacatta ttgcaaaaag ggggcattcg aacccccgcc
     5281 gatctcgatt atctccggca gttcgacagg gagtgtatcg aacgaaatct cagtccaggc
     5341 ggcagtgccg acctgctgat ccttacctgg tttttagcac agatttaatt atttaagcac
     5401 ttgataaatt tggaaata
//
LOCUS       DAZKGF010000245         5349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZKGF010000245 DAZKGF010000000
VERSION     DAZKGF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.245"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1294)
                     /locus_tag="ACHOQV_004692"
     CDS             complement(<1..1294)
                     /locus_tag="ACHOQV_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9632947.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTMTDRTNTRWGKYRPWLLFMSIPFGISIWLMFTTPNTDY
                     SVKLLWAWSTYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVQLGYQIAMGAMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLNGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAFMDYQPGV
                     EQTPQALWGICLLMTIIPAILNLITGLIMRFYLINNEYY"
     gene            1581..2333
                     /locus_tag="ACHOQV_004693"
     CDS             1581..2333
                     /locus_tag="ACHOQV_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9632948.1"
                     /translation="MIKIYKASKGQLKHREIFDEIKKKVDNGEWKEGKAIPTERELSE
                     YYLSSRTTIRKAIESLKQRGYLHSVHGQGTFVLPSRSRENHLLHSFTDDIKARGGIPA
                     QNILEIGYVPVSDVIRKNLDLDIHVQTVFCIKRVRYMGSTPLGIQTSWLALNDRQEIT
                     QEDLLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
     gene            2347..3423
                     /locus_tag="ACHOQV_004694"
     CDS             2347..3423
                     /locus_tag="ACHOQV_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP9632949.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKAQWGG
                     FLNAFVSPPADTFAGSLNGIAGLITDDSSQMYVFSEGLIEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWSGKKEKACHLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDIEAIASGLTLLALEAQEK"
     gene            3423..4421
                     /locus_tag="ACHOQV_004695"
     CDS             3423..4421
                     /locus_tag="ACHOQV_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9632950.1"
                     /translation="MNMPARTPVMPLAQTREEINATAASLRHLAAIWGECFSTFQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCYALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSMTVDALCHAVCSNAQVLGVTNCPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYALLRLAQKLCPGLTPLQIEDDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATALPLEEFYHSLRLHQAGVGQHYILLPATSCESPFYLATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            4426..5340
                     /locus_tag="ACHOQV_004696"
     CDS             4426..5340
                     /locus_tag="ACHOQV_004696"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP9632951.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGSDHEWDKEKALQAIAQSRYFYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYATEPDFTLLHDIQDEYQLDAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPVIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLPTPSEVNLFLSQHD"
BASE COUNT         1633 a         1155 c         1154 g         1407 t
ORIGIN      
        1 cgtaatattc attattaatc agataaaatc tcatgatgag tccggtaatc aggttaagta
       61 tcgcgggaat gatcgtcatt aacaggcaga tcccccacaa cgcctgtggt gtttgttcta
      121 cgccaggttg atagtccata aacgctaaag cgattccgat aaaccctccc gccagtccca
      181 taccaaactt ttgaaaaaag agaatcccgc caaatgccag tccagaaaca cgttgccccg
      241 ttttcatttc accataatcc actgactcag caatggatgc ccaataaatg ggaagctgaa
      301 tatcagtcag aaaggtcacc acaatataaa atgtaaaagc taacactata gaatttggtt
      361 gcacgaaata catcgcacca cccaccacga acgccagtaa ttgggtataa cgaaatattt
      421 ttattttgtc gtagagtcga gtaagccagg tgcaagcgat catagccaga acagaggcga
      481 tcacgcctga agttaaaaac ggtgaaatta aagcatcccc accattcagg taatattttg
      541 cataatatgc agcgacagag ttacgaataa tcccgccaaa cataatgatc gcaattacag
      601 cgcccaaaat gagccattga tcatttttga tcagtaaccg aaactgcgcg gccataccga
      661 gactgggttt tggatggtag atgcgctctc tcacgctaaa aaaacaatac aggcataaac
      721 acgttgccag aattcccatt gcccccattg ctatttgata acccagttgt acgttattct
      781 tcccaagata cattgcagat aaaggaacaa taattgttac ggcgaacatg gcaatttttg
      841 tcataacaaa acgataacca ttggcgctca atcgctcttg tggatcatcc gtgataacgc
      901 tgattaatga aacataagga atagcgatta acgtataggt caaggtaagt aacacatagg
      961 tagaccaggc ccataaaagc ttaactgaat aatcggtatt aggtgtggtg aacatcaacc
     1021 aaatgcttat cccaaacggt attgacataa aaagcaacca cggccgatat tttccccaac
     1081 gtgtattcgt tctgtctgtc attgttccca caactggatc aatcacagca tcaaggatac
     1141 gtacagaaaa taacagaata cctaagtcga cgggtttcag accaaataca tccgtgtaaa
     1201 aataagtgat gatcatggcc aaagacatca ttactatcgc gatagccata tcaccagcac
     1261 caaaacctat cttttcattt attgtaagtt tcatacacag cactcattga ttacgtgaac
     1321 ttatattaaa aacaaatata tctgaactca ttcagcacat aactttacga gtgagcataa
     1381 atgcattggt tgataccaca acaaaccatt gagtcaatat tgagcaacat cacataaaaa
     1441 ccaatttcac ttaatctcat gatttatata tttaattgtg gttttttctg ttttatgtca
     1501 cagatagtga acaagcacca tgcgtaataa aagttaaaga atgtagagtt ggtatcaacc
     1561 acttgaaatg aggctttgtc atgataaaaa tttataaagc cagtaaaggt cagctaaagc
     1621 atcgtgaaat ctttgatgaa ataaagaaaa aagtagataa cggtgaatgg aaagaaggca
     1681 aagccatacc gacagaaagg gaattgtcag aatattattt aagtagcaga acaactattc
     1741 gaaaggcaat agaaagtctt aaacaacgcg gctacctgca tagtgtccac gggcaaggaa
     1801 catttgtatt gccttctcgt agtagagaaa atcatttatt gcatagtttt accgacgata
     1861 ttaaagctcg aggtggtata ccagcacaga atattcttga gataggctat gttcctgtca
     1921 gtgatgttat cagaaaaaat ctcgacctgg atatccatgt ccaaaccgta ttctgtataa
     1981 aacgcgttcg ctatatggga agtacccccc tgggtattca gacatcatgg cttgcattaa
     2041 atgacaggca agaaataacc caagaagatc tgctggcgac gggttcgctc tatattttac
     2101 tggaagaaaa actcgctatt aaacctttag aagcaacaga aattatctct gcccggcttc
     2161 cctcgcccct tgagcgtcaa ttactggaac tgactgccga tgatgtcgta ttaagttgta
     2221 cccgcgtcac gttatccgtg gaacgcaaac caatggagta tgtagagatg gtgtatcccg
     2281 caagccggta ctcctacaaa gcacgaatta caaaagacag ttttagcgtg taacagagag
     2341 aatattgtga acaaaataga acaagctgta atttataaca aaatattagg ttctttagcc
     2401 tgcgcaggta tgggcgatgc attaggcgca gcgactgaac tctacagcat tgatgaaata
     2461 aaagctcaat ggggaggttt tttaaatgcc tttgtttctc ctcctgccga tacattcgct
     2521 ggctcattaa atggtattgc gggacttatc accgacgatt ccagccaaat gtatgtgttt
     2581 tcagaagggt taattgaggc tggatttgat aacttcacca ataatgactg gttagcttgt
     2641 cttctgcgtt gggccgatat gcagccctac gcaaattata aaggccccac aacagaacaa
     2701 atcgtcaagg cactaaaaga aggtagacca acaaacacca ttgggcgtat tggaacatct
     2761 tctcgtcagg cacccaatgt cggcaccacg aatggagcag gaatgcgtgt agcccctgca
     2821 ggattgatct ggtcaggcaa gaaagaaaaa gcgtgtcacc tggcgctaat aacttgccta
     2881 ccttcgcatg acaccaatat tgcgattgcc agcgcttgcg ccatcgctgc ggcgacatca
     2941 caagctatgt taccagaagc ttcgctcaca tcattactgg acgccgcaat ctggggagcg
     3001 aattacggcg aacgcctggc caaacaatat gcacgatgtg tcgcagggcc atctatcgcc
     3061 atgcgcatcc aacttgcagc ggacatcgcc agacgagcca atgacctgga gtcttgtctg
     3121 cgagaaatgg aaggtttggt tggtaactct gttgccgccc atgaatccat tcctgcggca
     3181 atcggtctat tgctgtactg caaaggagaa ccctgggaaa ccattcatgc ctgcgccaat
     3241 attggcaatg acaccgatag catcgctacg atggcaggcg caattgcagg ggcatggcgc
     3301 ggttttgatg cgctccctga agataaatat gcctttttcc gcgccgtaaa taataaagat
     3361 ttcgatattg aagcgatcgc atcgggtctg acattgcttg cattagaggc acaggagaaa
     3421 taatgaacat gccagcccgt actcctgtta tgccattagc gcaaactcgt gaggagataa
     3481 atgctacggc ggcatcgtta cgccatttgg cagctatctg gggtgaatgt ttttctactt
     3541 ttcagctcga taaacgtgct gttgaacgcg tctgtatagt gggatcgggc gactcctgga
     3601 cggtggcact atgtgccgcc gcctggctgg ggaaatacac acatctgttt tgttacgcat
     3661 tgcaaacgtg ggattttctt cagactgatt taacccgtta tcagaaagaa acgttaatca
     3721 ttattctcag tgcaagtggc agaccatcaa tgaccgttga cgccctctgc cacgcagtat
     3781 gctcaaacgc gcaagtgctg ggtgtgacga attgccctgg aacaccattt tgcgcaataa
     3841 cagaaaacat gctctataca tgggcgaata agcagggtat tccaacgcaa agtagtagcg
     3901 tcacacttta tgccctcctg cgcttagcgc aaaaattatg tccaggctta acaccactgc
     3961 agattgaaga tgatttggaa gggaaattta gccagattaa tcagcactgg cagcaaaaga
     4021 agcgccattt ttaccaacaa aaagagataa cgtttcttgg gagtggctta agttggggac
     4081 tggcaatatc ggggagcaat ttactttctt gcggcccaca gattcgtgcg acagcccttc
     4141 cactggaaga attttatcat tcattacgct tacatcaggc tggtgtcggt cagcattata
     4201 ttttactacc agccacatcc tgcgagtccc cattttacct tgcaacccaa aaagcaattg
     4261 ttgaacaggg agcgacagca gaattaatca gttttatacc tgacgccagt gagtccaaca
     4321 atttatttct ggtcatgcag tggttatatg aaatgtgctg gcatttaagt tgtgattatg
     4381 tcgacgcagg cgggcaacgc gttagccata gggagaaata agactatgaa gaaatttgat
     4441 gtcgccgctt taggcagtgg aaatatagat atttttttat ctatcccgtc actccccact
     4501 cgtggcggta aagttgtcgg gacacgtctg ggtgaacaag ctggcggcac agtcgccaac
     4561 agtgcctgtg cgatgggaca actgggtctc aatgtggtat ctgtttcatg tgtgggcaat
     4621 gatcattcag cctcgatcat tcttgatgga tttaaaaaat atcatgtaaa ttgtgatttc
     4681 gttcaggtaa ttccggaatt gattgccaat accgcgatta tctttattga tgaattaggt
     4741 gaaaaaacgt tggtttactc gcctggtagt gatcatgagt gggataaaga aaaagccctg
     4801 caggcaattg cgcaaagccg ttatttttat acgatgccag cagatatcga gaaatttcgt
     4861 atgctggccg aatattcccg tagccagatg acaaaagttg tcgttgatat tgagcctcat
     4921 atcattgcaa cacctgaaca ccttgccaga attttacacc ttgctgatat tgcgatattt
     4981 aattatgacg gttttattcg tggctatgca acagagccag attttacact tctgcatgat
     5041 atacaagacg agtatcaact tgacgctgtc gtggttacgc tggatgttag gggagtaatt
     5101 gcagtcaaag gcaatgagca agtaaaaata ggtagttata caattcccgt tattgatacc
     5161 acaggcgctg gagatacttt taatggtgca tttgtttata gcttaataaa aaatatgccc
     5221 ttaattgaag cactaaaatt tgcttctgcc acagcagcaa taaatattac tgcattaggt
     5281 gccagaggtc atctgccgac accatcggag gttaatcttt ttctttcaca acacgactaa
     5341 aatcatata
//
LOCUS       DAZKGF010000246         5310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZKGF010000246 DAZKGF010000000
VERSION     DAZKGF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.246"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(23..2605)
                     /locus_tag="ACHOQV_004697"
     CDS             complement(23..2605)
                     /locus_tag="ACHOQV_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9632952.1"
                     /translation="MKNSKAFYRSALATAIVMALSAPAFAADKAVSTDSVTLNKDKTT
                     LDQDVVINGDNKITAVTIETSDSDKDLNVTFGGHDITAASTVNQGFVEGVKVSGDKNV
                     VINATGSTITAQGEGTYVRSAMVISSTGDVVVNGGNFVAKNEKGSATGISLEGAQGNN
                     VTLNGTTINAQGNKSSSNASTAIFAQKGSVLNGFNGDATDNITLAGSNIINGRIETIL
                     IAQENKGTHTVNLKIKDGSVIGAANNKQTIYASASAQGKGSATQNLNLSVADSTIYSD
                     VLALSESENSAATTTNVNMNVARSYWEGNAYAYAYAYAYAGVYADDDKASSNLNINLS
                     DGSVWKGNVAGSTTTGSTGTTENQSATVSLSDSTWKGNVGDGLDGGARVTLKNSSWTG
                     DVGNAKTAVFLTDGSVWNGAVSINAPVDRASVPMGSVQPNKSSVSLQNGSVWNVTGAS
                     TVDALAVKDSTVNITKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLRVYSAGS
                     LDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSVI
                     QAMAAAPANVANLQADTLSARQDAVRLSENDEGGVWIQYFGGKQKHTTAGNASYDLDV
                     NGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGIF
                     IDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAKL
                     SALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNSTVKPYLNLAALNEFSD
                     GNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVGI
                     NVTW"
     gene            complement(2647..3408)
                     /gene="hyxA"
                     /locus_tag="ACHOQV_004698"
     CDS             complement(2647..3408)
                     /gene="hyxA"
                     /locus_tag="ACHOQV_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFP9632953.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLRENGNTNRYSVVANAGTQALSLHKYK"
     gene            complement(3562..4356)
                     /gene="hyxR"
                     /locus_tag="ACHOQV_004699"
     CDS             complement(3562..4356)
                     /gene="hyxR"
                     /locus_tag="ACHOQV_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFP9632954.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            complement(4686..5249)
                     /gene="ipbA"
                     /locus_tag="ACHOQV_004700"
                     /gene_synonym="fimX"
     CDS             complement(4686..5249)
                     /gene="ipbA"
                     /locus_tag="ACHOQV_004700"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFP9632955.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKCRPISRQHFFSIIREAGRRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT         1390 a         1294 c         1140 g         1486 t
ORIGIN      
        1 aggggaaccg actgctttat tcttaccagg tcacattgat accaactaca ccctgtagcg
       61 gattctcaat gtcgtcacct ttggtgtagt caaggcttgc atatgctccc atgtttttgg
      121 tgatatcagc ctgtacacct gcacccacgc ggaatgctgc accgtcaatg ctggcattga
      181 cagactcatc acccagacgg actttgttgc catcagagaa ttcgttcagt gcggccagat
      241 tcagataagg tttaacggtc gagttgccca cagcgaagtc ataacccacg cgcgtaccgg
      301 cctcacccag cacagagtta tagctgtcag aatgaacgtc cacgccgttg agttgataat
      361 ccacaccttc cagcgtcaga gcagacagtt tggcatatgg ctgaataaac aggccattac
      421 cgtctttcca tgtgtaaccg cctttaacca tcgcaccaaa accattggtg ttaaagtcag
      481 ctttgatggt accgccacca ttcatcaggc gaacatctgc cgtgttgctg taatgaccaa
      541 actgtgcagc agtatcaatg aagataccgt tgttatactg gcggctcagg taagcgtgga
      601 agctgtaacc ttcagtgtca cctttgctct gcatggtggt catgtcacct ttcgcagaag
      661 acatcgccac accggccagc cagctaccat cttcagtcat gaagcgggta tcaccaccca
      721 gcattacacc gtttacatcc aggtcatagg atgcatttcc tgcggtggta tgtttctgtt
      781 taccgccaaa gtactgaatc catacgccac cctcgtcatt ttcgctcaga cggacagcat
      841 cctgacgggc ggagagcgtg tcggcctgca gattagcgac gttagccgga gcagctgcca
      901 ttgcctgaat cacggagctg gcgttggatg ctttatgcgt gttttttacg aaataaaagt
      961 taccatcagc tccctgtgtc aggtcatatt gatacagacc accttccgtt gtgcctgtgg
     1021 cttttagagt gctgtctttg ccggtagaaa taaatgccgt ttgttcattg ataagatcca
     1081 atgaacccgc actgtaaaca cgcaagtcac cgctcgcttt accgctgata tccagggtgt
     1141 tttcagaaga ggcatcaaca atcagagtgc cgttttgaga agcaaacgtg ccagtattga
     1201 ctgtagcctt cgtgatatta accgtactgt cttttactgc cagagcatca acagttgagg
     1261 ctcccgtaac attccagaca gacccgtttt gcagagatac actgctttta tttggttgaa
     1321 ccgagcccat tggtactgat gcgcggtcca ctggcgcgtt aatggaaaca gcaccattcc
     1381 acacagaacc atccgtcaga aaaacagcag ttttggcgtt gccgacatca ccagtccagc
     1441 tggagttttt cagcgtgact cgcgcaccac catccagacc gtcacccacg ttacctttcc
     1501 aggtggaatc agacagactt acggttgctg actggttttc tgtagtaccg gtgctgccag
     1561 tagtcgtact acccgcaaca tttcctttcc agactgagcc atcggaaaga tttatattca
     1621 gattactact cgctttatcg tcatcagcat aaacaccagc ataagcataa gcataagcat
     1681 aagcataagc attaccttcc cagtaagagc gggcaacgtt catatttaca tttgttgtgg
     1741 tagcggctga attctcgctt tcagaaaggg ccaggacatc agagtagatg gttgaatcag
     1801 caacagacaa atttaaattt tgcgttgctg aaccttttcc ttgtgccgaa gctgaggcat
     1861 aaattgtttg tttattatta gccgccccaa ttactgagcc atccttaatc ttcaggttga
     1921 ctgtatgagt tcccttattc tcctgagcaa taagtattgt ttcaatccga ccattaataa
     1981 tatttgagcc agcaagggta atgttgtctg ttgcatcacc gttaaaacca ttcaatacgc
     2041 tacccttttg agcaaaaatt gccgtggagg cgttgctgga actcttatta ccttgagcat
     2101 ttatggttgt gccattaagc gtgacattat ttccctgggc cccttccaga gatatccctg
     2161 tcgcactacc tttttcattt tttgcaacga aattaccgcc attaacaaca acatcgccag
     2221 ttgaagaaat gaccattgca gaccggacat aggtgccttc accttgagct gtgatggtgg
     2281 agcctgtagc attaatcaca acatttttgt caccgctaac ttttacacct tcaacgaaac
     2341 cttggtttac cgttgatgcg gcggtaatat cgtgaccgcc aaaagtaaca ttaaggtctt
     2401 tatctgaatc tgacgtttca attgttacgg ctgtaatctt gttatcaccg ttaataacaa
     2461 catcttgatc cagagttgtc ttatctttat tcagcgtaac cgaatcagtt gataccgctt
     2521 tatcagcagc gaatgctggt gcagaaagag ccataacaat agctgttgct aatgcgctgc
     2581 ggtaaaatgc cttactgttt ttcatgaaga aattctccat tctggtaact aatttcaatt
     2641 tcccacttat ttatatttat gcagagataa agcctgcgtc cctgcatttg ccaccacgga
     2701 atacctgttg gtatttccgt tttcacgcaa tgtcatggca ttcgttgttt ccaatgcatt
     2761 tccatcaccg tgaaaattat tgctgctcag cagcagggct tcccgcaatg acatgctgtc
     2821 cggtattccc caacgctttt ttagcgactg ctcggcaagc catatctgtc ttatccccgt
     2881 tttgagaaac tgcgcactgt attcccagct gtgtccggca agaagcaacg acaacacctt
     2941 cttttgcttt tccgggaagc gttctgacat gacgattgtc tttgtgcggt tattcacctc
     3001 tttcataaag acagagtaac tgtccataac tggcaaaatt tctgcatgtt tcagccctga
     3061 aaacccacca gaggcatttg caatactgat gacacgctct actgacattc ctggtttcag
     3121 taacctcagg tttttgagaa cgccagaaat actcacatct ggtggcagaa aaaagggtgc
     3181 gacgcacaac accaccacga atgagtgatc ccgataaaat cggaaccaca tttcagcgca
     3241 ttcggcaggt cgccctgccg atatcacgac actctgtggc ctgtgccgca atttattgcg
     3301 taactcctct tctgttctga tgcaggtaac attgccgaca gaaccggttg atagagctgc
     3361 gaactgcgct ggtacaattc ctgagggaca gaaaagaacg aaacgcatcc ttccctcaca
     3421 gatgtcagta cgccagcacg ctgtacaggc tgtacagcgt gcctcccaat cctgcagccc
     3481 agagcagtag aaatattttg gctcctacac tgtagcgagt ccgtatgcgc cgcagtgggg
     3541 ggtagtaaca acgctgctgt gtcataacaa tcctggtctt tttttagaca tattttttct
     3601 ttgtccggaa agtacttttc cggatatggc gtcagaattt attgattcac caacggcaac
     3661 tttctctgga taaagaattc cggataccat gttctgcaca tactgagtga gtcgatgagc
     3721 ggaatgttgt ccgatatatt ttgcatgtag ccgtatattc cgtttcactc tgctgtcacc
     3781 gcatcccgta tagagcagcc tgaaatcgct cagtgttccc agtccaagtt tcattaaagc
     3841 ccgtgtggca atcgcatgca gggtctttga actgcgttgt tcataacggg caatctcact
     3901 gaaactcata ccctgcatca gaatcttaag cacgctcaac tcccgtgcag tcaggagcca
     3961 gaaatcgctc atatcgccgg gcaaaggacg tagtaaccag cggataattt gattctgata
     4021 acgactcgct gtcagcatgg tgccgatgcg tgggaagccg ttatacagac ttgcagcagg
     4081 agcattatcg aaccagtcgg agtcaccggt caggcagacc tgccattgcc cggcatcact
     4141 ccgcaagcgg acaagacaag ccagaagaac caactctccc gaaggaagcg atgggatatc
     4201 agctaacagg tagccacagg aggtctttaa aacgaattca acctctgtag tgacgttgct
     4261 gtacacccgc acgatatacc tgtccgttgg gatgagagac tccagtgcca gcctggtgaa
     4321 ctgacaggca ctgataataa ccagaggctt cttcattcag actcgtccac gccattgcta
     4381 caaaacctga cattttgatt caaaatgtca ggtttggttt aattgcctga atatttaatt
     4441 ttattggaat ataaatctaa tttgtttgat ttttattgtt tttaattttg tgttatttaa
     4501 caacgcgagt gaaaactgat cactggtatg acctttgtgg ttaatcattt caaatcatgg
     4561 ggttatttta ctttagcgag aaatatcaac atgttatgtg ttttatttaa ttgaatgagc
     4621 tgttttttta tggaagaatt gaggcaaacg taggaatctg acattttgaa tcagaaggta
     4681 ctgttttaac gaggcttttt tttccacacc cctttaaatc gagcagcatt actggctgtg
     4741 tatctgacag tatgctgaat atttcgatga cccagataat cctgaagtag tcgggtatcg
     4801 acaccattgt cagccagagc aaatccacag gcatgacgta acatatgagg atgtgctttt
     4861 acagctaatc ctgcacgtct tcctgcctca cgaatgatcg aaaaaaaatg ttgtctgctg
     4921 atagggcggc atttccggga gagaaaaagc cagtctcctt caactccatt ttcaattaac
     4981 ttcctctgct taagccacaa cttgatcaaa ttatattcat caggaagaag aggatgtgtt
     5041 gtcgagaagc catttttaat acgccggata ttaagttgtt ttcctgatgc atcaatatcg
     5101 gataaacgta aatcaagaag ttcactggcc ctaaaaccgt gaatgaaagc catcataatt
     5161 agacagcgat ttctttcagg aaaggacatg ctctgggctg catccatcag cctgtagact
     5221 tcatcctgtg tgagatattt tctcgtcata cgccaccgtg tacagttgta aaaaaaagag
     5281 gcacacccag ataagtgtgc ctgcaatcag
//
LOCUS       DAZKGF010000247         5287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZKGF010000247 DAZKGF010000000
VERSION     DAZKGF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.247"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            100..714
                     /gene="evgA"
                     /locus_tag="ACHOQV_004701"
     CDS             100..714
                     /gene="evgA"
                     /locus_tag="ACHOQV_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFP9632956.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            719..4312
                     /gene="evgS"
                     /locus_tag="ACHOQV_004702"
     CDS             719..4312
                     /gene="evgS"
                     /locus_tag="ACHOQV_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFP9632957.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(4368..>5287)
                     /gene="yfdE"
                     /locus_tag="ACHOQV_004703"
     CDS             complement(4368..>5287)
                     /gene="yfdE"
                     /locus_tag="ACHOQV_004703"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFP9632958.1"
                     /translation="DLKNDHDKSIFINMLKQADVLAENFRPGTMEKLGFSWERLQEIN
                     PRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYPDAPPVRVGTSLADLCGGV
                     YLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMAYIATGKSPQRLGNRHPYM
                     APFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPRFSSNILRVQNQAILKQYI
                     ERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQARNMLIEAGGIMMPGNPIK
                     ISGCADPHVMPGAATLDQHGEQIRQEFSS"
BASE COUNT         1607 a         1148 c         1056 g         1476 t
ORIGIN      
        1 gctcaaattt tcttattttg attgagaaaa tgagatgatg ccttatgtct gtattactac
       61 agggagaagg gaaatgcttc attgcaaagg gaataatcta tgaacgcaat aattattgat
      121 gaccatcctc ttgctatcgc agcaattcgt aatttattga tcaaaaacga tattgaaatc
      181 ttagcggagt tgactgaagg gggaagtgcc gttcagcggg tggaaacact taaacctgat
      241 atcgtcatca ttgatgtcga tatccccgga gttaacggta tccaggtgtt agaaacgctg
      301 aggaagcgcc aatatagcgg aattattatt atcgtctccg ctaaaaatga ccatttttac
      361 gggaaacatt gtgctgatgc tggcgctaat ggtttcgtga gtaaaaaaga aggcatgaac
      421 aatatcattg cagctattga agctgcaaaa aatggctact gctatttccc cttctctctc
      481 aaccggtttg ttggaagttt aacgtccgac cagcaaaaac tcgactcctt atcgaaacaa
      541 gaaattagtg tcatgcggta tattcttgat ggcaaggata ataatgacat tgctgaaaaa
      601 atgttcatca gcaacaaaac tgtcagcact tataaaagtc gcttgatgga aaaattagaa
      661 tgcaaatcac tgatggatct ttacacattc gcacaacgta acaaaatcgg ctaaccacat
      721 gaagttttta ccctatattt ttcttctctg ttgtggtctt tggtcgacca taagtttcgc
      781 agacggagat tacatcgaat atcgtggcat cagtagtaac aaccgtgtca cacttgatcc
      841 actacgtctg agcaacaagg aattacgttg gctcgcgagc aaaaaaaatc ttgtgattgc
      901 agtacacaag tcccaaacag ctacgttgtt gcataccgat tcgcagcaac ggattcgtgg
      961 tattaatgct gattatttaa atcttttaaa aagagcgtta aatatcaaat taacactccg
     1021 ggaatacgca gatcatcaaa aagcaatgga cgcgctggaa gacggtgaag ttgatatagt
     1081 gttatcacat ttagttgctt cgccgcctct taatgatgac attgctgcaa ccaatcctct
     1141 gataattacc tttccggcgc tggtcactac ccttcacgat tcaatgcgac cgcttacctc
     1201 atcaaaacca gtaaatattg ctcgagtagc aaattatccc ccagacgagg taattcatca
     1261 atcatttcca aaagcaacaa ttatctcttt tacaaattta tatcaggcat tagcatccgt
     1321 ctcagccgga cagaatgatt actttattgg tagtaacatc attaccagca gtatgatttc
     1381 ccgttatttt actcactcct taaatgtagt gaagtattat aactcaccgc gtcaatataa
     1441 ctttttatta accagaaaag attctatcgt tcttaatgaa gtactcaatc gatttgttga
     1501 tgctttaaca aatgaagttc gctatgaagt atcacaaaat tggcttgata caggaaatct
     1561 ggcctttctg aacaaaccat tagaactcac tgaacatgaa aaacagtgga ttaagcagca
     1621 tcccgattta aaggtgctag aaaatcctta ctcgccaccc tattctatga cagatgaaac
     1681 tggctcagtt cggggcgtga tgggtgatat tcttaatatt attaccttgc aaacaggttt
     1741 aaatttttct ccgatcaccg tttcacacaa tattcatgct gggacacagc ttaaccccgg
     1801 tggatgggat atattgcccg ccgctattta tagtgaagat cgagaaaata atgtttcatt
     1861 tgctgaagcc ttcataacaa cgccttacgt ttttgtcatg caaaaagcgc ctgacagtga
     1921 acaaacatta aaaaaaggaa tgaaagttgc cattccctat tattatgagc ttcattcgca
     1981 attaaaagag atgtatccgg aggtagagtg gataaaagtc gataacgcca gcgctgcatt
     2041 tcacaaggtt aaagaaggtg aacttgatgc tctggtcgcg acacagttaa attcgcgtta
     2101 catgatcgac cattactatc ctaatgaact ttatcatttt cttattcccg gcgttcagaa
     2161 tgcatcactt tcgttcgctt ttcctcgcgg agaaccggaa cttaaggata ttattaataa
     2221 agcactgaat gcaattcccc caagcgaagt tctgcgcctg accgaaaaat ggattaaaat
     2281 gcccaatgtg accattgaca catgggacct ctatagcgag caattttata ttgttacgac
     2341 attatccgtt ttattagttg gcagtagcct tttatgggga ttctacctgt tacgctcagt
     2401 tcgtcgtcgt aaagttattc agggtgattt agaaaaccaa atatcattcc ggaaagcgct
     2461 ctcggattcc ttaccgaatc caacttatgt tgtaaactgg caaggtaatg tcattagtca
     2521 caatagtgca tttgaacatt atttcactgc tgattactat aaaaatgcaa tgttaccatt
     2581 agaaaacagt gaatccccct ttaaagatgt tttttctaat acgcatgaag tcacagcaga
     2641 aacgaaagaa aaccgaacaa tatacacaca ggtatttgaa attgataatg gcatcgagaa
     2701 aagatgcatt aatcactggc ataccttatg taatctgcca gcaagcgaac atgctgttta
     2761 tatttgtggt tggcaagata ttaccgagac gcgtgattta attcatgcac tcgaagtaga
     2821 gagaaataaa gcgatcaatg caactgtcgc aaaaagccag tttctggcaa caatgagtca
     2881 cgaaataaga acaccaataa gctccattat gggcttcctg gaacttctgt cgggttctgg
     2941 tcttagcaag gaacaacggg tggaggcgat ttcacttgcc tacgccaccg gacaatcact
     3001 cctcggctta attggtgaaa tccttgatgt cgacaaaatt gaatcgggta actatcaact
     3061 tcaaccacaa tgggtcgata tccctacttt agtccagaac acttgtcact ctttcggtgc
     3121 gattgctgca agcaaatcga tcgcattaag ttgcagcagt acgcttcctg aacgctacct
     3181 ggttaagatc gaccctcagg cgtttaagca ggtcttatct aatttactga gtaatgctct
     3241 caaatttact accgaggggg cagtaaaaat tacgacctcc ctgggtcaca ttgatgacaa
     3301 ccacgctgtt atcaaaatga cgattatgga ttctggaagt ggattatcgc aggaagaaca
     3361 acaacaactg tttaaacgct atagccaaac aagtgcaggt cgtcagcaaa caggttctgg
     3421 tttaggctta atgatctgca aagaattaat taaaaacatg cagggcgatt tgtcattaga
     3481 aagtcatcca ggcataggaa cgatatttac gatcacaatc ccggtagaaa ttacccaaca
     3541 agtggcggct gtcgaggcaa aagcagaaca acctataaca ctacctgaaa agttgagcat
     3601 attaatcgcg gatgatcatc cgaccaacag gctattactc aaacgccagc taaatctatt
     3661 aggatatgat gttgatgaag ccactgatgg tgtgcaagcg ctacacaaag tcagtatgca
     3721 acattatgat ctgcttatta ctgacgttaa tatgccgaat atggatggtt ttgagttgac
     3781 tcgcaaactc cgagagcaaa attcttcctt acccatctgg gggcttacag ccaacgcaca
     3841 ggctaacgaa cgtgaaaaag ggttaaattg cggcatgaac ttatgtttgt tcaaaccgtt
     3901 gactctggat gtactgaaaa cacatttaag tcagttacac caggttgcgc atattgcacc
     3961 tcagtatcgc caccttgata tcgaggccct gaagaataat acggcgaatg atctacaact
     4021 gatgcaggag attctcatga ctttccagca tgaaacgcat aaagatctac ccgctgcgtt
     4081 tcatgcacta gaagctggcg ataacagaac tttccatcag tgtattcatc gcatccacgg
     4141 tgcggctaac atcctgaatt tgcaaaagtt gattaatatt agccaccagt tagaaataac
     4201 acccgtttca gatgacagta agcctgaaat tcttcagttg ctgaactctg taaaagaaca
     4261 cattgcagag ctggaccagg agattactgt tttctgtcag caaaatgact aaatagcggc
     4321 tcccacaatg ttcaaatgtg ggagctattt accagcacat cttacattta tgatgagaac
     4381 tcctggcgaa tttgttcccc atgctggtcg agcgttgccg ctcccggcat aacatgcggg
     4441 tccgcgcagc cgctgatttt tatcggattc cccggcatca ttattccccc ggcttcaatc
     4501 aacatatttc tcgcctgagt ttgtggcaaa ttaatggcct cagccacact taataacggc
     4561 gcgacgggta caccaacttc atgtattctg gctaaccaaa cttcagctgc ctgcgttttt
     4621 aacgtccgct caatatattg tttaagaata gcctggtttt gtacgcgtaa aatattgctg
     4681 ctaaatctgg gatcattaac cagttccgta agctccagtg cctggcataa cgcagaaaaa
     4741 agcttgtcat taccacaaca aatcgtaatc ggcttatcct gagtatcgaa aacatcaaaa
     4801 ggtgccatgt agggatggcg atttcccagg cgttgtggtg acttccctgt cgcaatatat
     4861 gccatcagac catgctccag aaaactcagc gtggcatcaa acatcgctat atcgacatgc
     4921 gcccctcttt ggctcttttc gcggccataa agtgcactca ctattccgct gaataaatag
     4981 acaccgccgc atagatccgc aagagaggta cccacgcgca ctggcggagc atcagggtat
     5041 cctgtttcca tcattatccc gctcattgcc tgaatgatgg tatcgtaggc aggagcatct
     5101 tttagcggac cggtatgtcc gaaacctgac gatgaagcat atataaggcg cgggttgatt
     5161 tcttgtaacc tttcccatga aaacccgagt ttttccattg tacctgggcg aaaattctca
     5221 gctaatacat ccgcttgttt aagcatattt ataaatatac ttttatcgtg atcattcttt
     5281 aaatcaa
//
LOCUS       DAZKGF010000248         5265 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZKGF010000248 DAZKGF010000000
VERSION     DAZKGF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.248"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            120..923
                     /gene="dkgB"
                     /locus_tag="ACHOQV_004704"
     CDS             120..923
                     /gene="dkgB"
                     /locus_tag="ACHOQV_004704"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFP9632959.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(920..1834)
                     /gene="yafC"
                     /locus_tag="ACHOQV_004705"
     CDS             complement(920..1834)
                     /gene="yafC"
                     /locus_tag="ACHOQV_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFP9632960.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            2075..2875
                     /gene="yafD"
                     /locus_tag="ACHOQV_004706"
     CDS             2075..2875
                     /gene="yafD"
                     /locus_tag="ACHOQV_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9632961.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2952..3722
                     /locus_tag="ACHOQV_004707"
     CDS             2952..3722
                     /locus_tag="ACHOQV_004707"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9632962.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARHLKNIATRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDVWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(3769..5127)
                     /gene="mltD"
                     /locus_tag="ACHOQV_004708"
     CDS             complement(3769..5127)
                     /gene="mltD"
                     /locus_tag="ACHOQV_004708"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFP9632963.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(5199..>5265)
                     /locus_tag="ACHOQV_004709"
     CDS             complement(5199..>5265)
                     /locus_tag="ACHOQV_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632964.1"
                     /translation="ETLLQQPEERFAWLRSKKDRF"
BASE COUNT         1302 a         1312 c         1274 g         1377 t
ORIGIN      
        1 ctgtatttct attattgcca gaatcgcaaa aatcctctgc gttttacgct ctttttccgc
       61 aacagtctga agcccataat cacctcagtt aacgaaaata gcattaaaag aggcatatta
      121 tggctatccc tgcatttggt ttaggtactt tccgtctgaa agacgacgtt gttatttcat
      181 ctgtgaaaac ggcgcttgaa cttggttatc gcgcaattga taccgcacaa atctatgata
      241 acgaagccgc agtaggtcag gcgattgcag aaagtggcgt gccacgtcat gaactctaca
      301 tcaccactaa aatctggatt gaaaatctca gcaaagacaa attgatcccg agtctgaaag
      361 agagcctgca aaaattgcgt accgattatg ttgatctgac gctaatccac tggccgtcac
      421 caaacgatga agtctctgtt gaagagttta tgcaggcgct gctggaagcc aaaaaacaag
      481 ggctgacgcg tgagatcggt atttccaact tcacgatccc attgatggaa aaggcgattg
      541 ctgctgttgg cgctgaaaac atcgctacta accagattga actctctcct tatctgcaaa
      601 accgtaaagt ggtcgcctgg gctaaacagc acggcatcca tattacttcc tatatgacgc
      661 tggcgtatgg taaggccctg aaagatgagg ttattgctcg tatcgcagct aaacacaatg
      721 cgactccggc acaagtgatt ctggcgtggg ctatggggga aggttactcc gtaattcctt
      781 cttctactaa acgtaaaaac ctggaaagta atcttaaggc acaaaattta cagcttgatg
      841 ccgaagataa aaaagcgatc gccgcactgg attgcaacga ccgcctggtt agcccggaag
      901 gtctggctcc tgaatgggat taagcctctc tgacagctcc tccgggagct gtttttacat
      961 gctcgctaag gaaatcgata aaagcccgga tgcgcgtact taccgcacgg tcgctgtaat
     1021 atacggcact gaatggcatt tccactggca atactttatc tgccattaac tccaccaatt
     1081 ctccgcgagc gatttctctg tcgatcatgt agtcggacaa acacgcaatc ccgttgccac
     1141 tcaggcaaag ctgtttcagt gtttccccac tattggatga caaaccgtac ttcacctcat
     1201 gtaattgtcc atcactacag gctatcggcc aggtattgag ggaagcgggt tcagtgaatc
     1261 ccaggcaaac atgttgcttt aaatcgtcga tcgtttctgg cttcccgtag cgggaaatat
     1321 aatcggggga ggcgataatt tttcgataac tgttaaataa cggcctggca cgtaagctgg
     1381 aatccgttaa cgtaccagcg cgtatcgcga catccacttt tctttcgatc aaattaataa
     1441 tcgtttcgga ggagactagc gataaagtga cttccggata gcgttcacga aaaggcttaa
     1501 tcaacggcat cagaaagtgc agcaccactg gagttgcggc atcgatccgt aacagtccac
     1561 gcggtgtatt acgcgtctcc ataatttctg attctgccgc tgccatctcc tgcaaaattg
     1621 actgtacgcg acgaaaataa cgctcgcctt cttccgtcag gctaagttgc cgcgtggtcc
     1681 gattaagcag gctaacgcca agtttcatct ccagcttttt caccgcccgg cttaccgctg
     1741 agtttgcttg ccctaattgt tccgctgccc ggctaaagct gccgctttcg acgactgaaa
     1801 caaaaatggc gagttcttcc gacgtggctt tcatttttgc tcctgttgca aaatagaaga
     1861 gatattttga atttaattgt cattaaacca tcaggatgcg agatatgtca tccgatttaa
     1921 tgttctcaat aatgagcaaa tttctgaccg gtgtaagcac ttgcttacat agcaatatac
     1981 aattgctcgt tgaaagagtg agctaaaatc cctataacag tagaaccctc ccgagtgcgg
     2041 aagggttgac gtaatagagg tttcaaagtc aaaagtgcga aaaaacacct atgccatgcg
     2101 ctatgttgct ggacaacctg cggaaaggat cttaccgccg gggtcttttg cgagcatcgg
     2161 ccaggcatta ccacctggag aaccgttaag taccgaagag cgtattcgga tcctggtgtg
     2221 gaacatatac aaacagcaac gcgctgaatg gttgtcggta ttaaagaact acggcaaaga
     2281 tgcacatctg gtgttattgc aggaagcgca gacaacgcca gagttagtgc agtttgcgac
     2341 cgctaactat cttgccgccg atcaggtacc cgctttcgtg ctgccacaac atccttccgg
     2401 tgtaatgacc ctttcggcgg cgcatccagt gtattgctgc ccgttacgtg aacgagaacc
     2461 cattttgcgt ctggcgaagt cggcactggt gacggtctat ccattgcctg acacccgcct
     2521 gttgatggtg gttaatatac acgccgtcaa tttcagtttg ggcgtggatg tctatagtaa
     2581 gcagttactt cctattggcg atcagatagc tcaccacagc ggcccggtca ttatggcggg
     2641 agatttcaat gcctggagcc gtagaaggat gaacgcgtta tatcgctttg cgcgggaaat
     2701 gtcgctgcgc caggtgcgtt ttaccgatga tcagcgccgt cgggcgtttg gccgcccgct
     2761 cgattttgtt ttctaccgtg gtctgaacgt cagcgaagct tctgtactgg ttacgcgcgc
     2821 ttccgatcac aatccgctac tcgttgaatt cagtcccggc aagcctgata aataaggtat
     2881 gtcaggtctg ccacagggca gaccaacgtt tggcgctgcc ctttttaatc aaacaataaa
     2941 aggacagcgc aatgacaaca caatcccacc atgaccacgt agaaaagcag tttagctcgc
     3001 aggccagtga gtatttaacc agtaccgtac atgcatccgg gcgagattta cagcgcctgg
     3061 cggtgcgtct ggctgattat cctgatgcaa gtgtacttga tatgggctgc ggagcagggc
     3121 atgccagctt tgtcgctgcg cagaacgtga gcgcggtggt ggcgtatgac ttatctgccc
     3181 aaatgctgga tgtcgtggca caagctgccg aagcccggca cctgaaaaat atcgccaccc
     3241 gccagggata tgccgaaagt ctgccatttg ccgataacgc atttgatatt gttatcagcc
     3301 gttattctgc ccatcactgg catgatgttg gtgcagcact gcgagaagtg aataggatat
     3361 tgaaacctgg cggtaggctg attgtgatgg acgtaatgtc tccgggtcac ccagtgcgcg
     3421 acgtctggtt acagacggta gaagcattac gcgatacctc tcacgtacga aactacgcca
     3481 gcggtgagtg gttgacgtta atcaatgaag ccaatctgat agttgataat ttaattacag
     3541 ataagttacc gctggaattt tcttcatggg tcgcgagaat gcgtacgcca gaagcgttag
     3601 tagacgctat tcgcatttac caacagagcg catcgacaga ggtgaaaacg tattttgcct
     3661 tgcagaatga tggctctttc accagtgata tcatcatggt agaagcacat aaagcggcat
     3721 aaataaaaaa ggcaccgggg gaatcggtgc cttttattat ctggtttgtc aggaatctgg
     3781 catgttgttg tttttcacaa acaacgtcag cttatcgcct ggttgcagat tcgcagtgtc
     3841 gctgttccag cgcattacat ctttgatgtt cacgccgtgg cgtttagcaa tgcttgaaag
     3901 cgaatcgcct ttgcgcacac gatacgtaat gctatcgctg ttgtttgcca gtcgctgtgc
     3961 gctactgcct gcaccaatcg tcaaactttg gcctggcttc agcttagatc cgcgcagttt
     4021 gttccactgc tgcaaatctt tggtgcttac gccgagacgt gaagcgatac ttgaaagcgt
     4081 gtcgccagag cgtacggtgt aaacacggct gttaagcggc gtattgtcgg caaccagcgt
     4141 cgactgtaca gcagcaattt cgcctgaagc cagagattca cgcagttgat ctgcatgctt
     4201 ctttggcacc atcacgtact gcggaccact tgcgcccagc gtggagcctt tcacgcctgc
     4261 gttgaatgtc ttcagcttgc tgacggaaat ccccgccata tctgcaacct tcgccatttc
     4321 aaccgggctg ctcaggtgca cacgtgccag agcacggctt tcatcggtcg ttggcagacg
     4381 tacgccataa cgcttgctgt ttttgagaat atcactcaat gccagcatct taggcacgta
     4441 ctgcttcgtt tcctgcggca acggtaacga ccagaagtcc gtggatttcc cacgcgcttt
     4501 gttcgtttta attgccttca tgactcgacc ttcgccgctg ttataagcag ctacggtcag
     4561 aagccagtcg ccgtcaaaca ttttgttcag acgctgcatc atgttcagcg cggcagttgt
     4621 tgaagcaaca acatcgcgac gcgcgtcata attgcgggtc tgtttcaaac cataattgcg
     4681 ccccgtgctc ggaatgatct gccagatgcc tgcggcattg gcgccagacg ttgcgtgagg
     4741 atcaaaagcg ctctccacta tgggtagtag taccagttcc ataggcatgt tacgtttttt
     4801 aacttgcccg gctatccagt acatatacgg ctctgcccgt aaagttacat cgtggagata
     4861 gctcttattg cgtaaatatt tctgtttctg ttcgcgaatc cggtcatttt ccggaattcc
     4921 catctttagc tcgtcgccaa tgaaagccca caagtcacca tctggcgcga tagacgtccc
     4981 atcgtccatc catcgtgcct gacttgtaaa ctttgctgct tccccttgac cagctgcaga
     5041 aaggctctgt gcgtgctgtt gaacgttgcc ggtactctgg caacccacga gcaggacaga
     5101 ggcgagtaat atcgcttttg ccttcatgtg tgtgtcaata gttgcttaaa agacgaccga
     5161 tcataacggc gaacggagcc gatgacaaga aagttttatc agaacctatc tttctttgac
     5221 cttaaccatg caaaacgttc ttcaggttgt tgcaataatg tttct
//
LOCUS       DAZKGF010000249         5254 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZKGF010000249 DAZKGF010000000
VERSION     DAZKGF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.249"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            238..2943
                     /gene="malT"
                     /locus_tag="ACHOQV_004710"
     CDS             238..2943
                     /gene="malT"
                     /locus_tag="ACHOQV_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFP9632965.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGDWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWHNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(2986..4002)
                     /gene="rtcA"
                     /locus_tag="ACHOQV_004711"
     CDS             complement(2986..4002)
                     /gene="rtcA"
                     /locus_tag="ACHOQV_004711"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFP9632966.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSSTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVALFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(4006..5232)
                     /gene="rtcB"
                     /locus_tag="ACHOQV_004712"
     CDS             complement(4006..5232)
                     /gene="rtcB"
                     /locus_tag="ACHOQV_004712"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFP9632967.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT         1240 a         1492 c         1311 g         1211 t
ORIGIN      
        1 ttaaatgaaa ctgatgaaca taaggaaaat accgtacaac ccagtattca cgctggatca
       61 gcgtcgtttt aggtgagttg ttaataaaca tttggaattg tgacacagtg caaattcaga
      121 cacataaaaa aacgtcatcg cttgcattag aaaggtttct ggccgacctt ataaccatta
      181 attacgaagc gcaaaaaaaa taatatttcc tcattttcca cagtgaagtg attaactatg
      241 ctgattccgt caaaattaag tcgcccggtt cgactcgacc ataccgtggt tcgtgagcgc
      301 ctgctggcta aactttccgg cgcgaacaac ttccggctgg cgctgatcac aagtcctgcg
      361 ggctacggaa agaccacgct catttcccag tgggcggcag gcaaaaacga tatcggctgg
      421 tactcgctgg atgaaggtga taaccagcaa gagcgtttcg ccagctatct cattgccgcc
      481 gtgcaacagg caaccaatgg tcactgcgcg atatgtgaga cgatggcgca aaaacggcaa
      541 tatgccagcc tgacgtcact cttcgcccag cttttcattg agctggcgga atggcatagc
      601 ccactttatc tggtcatcga tgactatcat ctgatcacta atcctgtgat ccacgagtca
      661 atgcgcttct ttattcgcca tcaaccagaa aatctcaccc ttgtggtgtt gtcacgcaac
      721 cttccgcaac tgggcattgc caatctgcgt gttcgtgacc agctgctgga aattggcagt
      781 cagcaactgg catttaccca tcaggaagcg aagcagtttt ttgattgccg tctgtcatcg
      841 ccaattgaag ccgcagaaag cagtcggatt tgcgatgacg tttccggttg ggcgacggca
      901 ctgcagctaa tcgccctctc cgcccggcag aatactcact cagcccataa gtcggcacgc
      961 cgcctggcgg gaatcaatgc cagccatctt tcggattatc tggtcgatga ggttttggat
     1021 aacgtcgatc tcgcaacgcg ccattttctg ttgaaaagcg ccattttgcg ctcaatgaac
     1081 gatgcactca tcacccgtgt gaccggcgaa gaaaacgggc aaatgcgcct cgaagagatt
     1141 gagcgtcagg ggctgttttt acagcggatg gatgataccg gcgattggtt ttgctatcac
     1201 ccactgtttg gtaacttcct gcgccagcgc tgccagtggg aactggcggc ggagctgccg
     1261 gaaatccacc gtgccgccgc agaaagctgg atggcccagg gatttcccag cgaagcgatt
     1321 catcatgcgc tggcggcagg cgatgcgctg atgctgcgcg atattctgct taatcacgcc
     1381 tggagtttgt tcaaccatag cgaactgtcg ctgctggaag agtcgcttaa ggccctgccg
     1441 tgggacagtt tgctggaaaa tccgcagttg gtgttattgc aggcgtggct gatgcaaagc
     1501 cagcatcgct acggcgaagt taacacccta ctggcccgcg ctgaacatga aatcaaggac
     1561 attagagaag gcaccatgca cgcagaattt aacgctctgc gcgcccaggt agcgattaac
     1621 gatggtaatc cggatgaagc ggaacggctg gcaaaactgg cactggaaga gctgccgccg
     1681 ggctggttct atagccgcat tgtggcaacc tcggtgctgg gtgaagtgct gcactgcaaa
     1741 ggcgaattga cccgctcact ggcgctaatg cagcaaaccg aacagatggc acgccagcac
     1801 gacgtctggc actacgcctt atggagttta atccagcaaa gtgaaattct gtttgctcag
     1861 ggattcctgc aaaccgcgtg ggaaacgcag gaaaaagcgt tccagctgat caacgagcag
     1921 catctggaac agctgccaat gcatgagttt ctggtgcgca tccgagcaca actgttatgg
     1981 gcctgggcac ggctggatga agccgaagcg tcggcgcgta gcgggattga agtcttgtcg
     2041 tcttatcagc cacagcaaca gcttcagtgc ctggcaatgt tgattcaatg ctcgctggcc
     2101 cgtggtgatt tagataacgc tcgtagccag cttaaccgtc tggaaaacct gctggggaat
     2161 ggcaaatatc acagcgactg gatctctaac gccaacaaag tccgggtgat ttactggcaa
     2221 atgaccagcg ataaagccgc cgctgccaac tggttgcgtc atacggctaa accggagttt
     2281 gcgaacaacc acttcctgca aggtcaatgg cacaacattg cccgtgcaca aatcttgctg
     2341 ggcgagtttg aaccggcaga aattgttctc gaagaactca atgaaaacgc ccgcagtctg
     2401 cggttgatga gcgatctcaa ccgtaacctg ttgctgctta atcaactgta ctggcaggcc
     2461 ggacgtaaaa gtgacgccca gcgtgtgttg ctggacgcat taaaactggc gaatcgcacc
     2521 ggatttatca gtcattttgt catcgaaggc gaagcgatgg cgcaacaact gcgccagctt
     2581 attcaactca atacgctgcc ggagctggaa cagcatcgcg cacagcgtat tctgcgagaa
     2641 atcaatcaac atcatcggca taaattcgcc catttcgatg agaatttcgt tgaacgtctg
     2701 ctaaatcatc cggaagtgcc agaactgatc cgcaccagcc cgttgaccca acgtgaatgg
     2761 caggtcctgg ggctgattta ctccggttac agcaacgagc aaattgcagg tgagctggaa
     2821 gtggcggcaa ccaccatcaa gacgcatatc cgcaatctgt accaaaaact cggtgtcgcc
     2881 caccgtcagg ccgcggtaca acacgcgcag aaactgctga agatgatggg atatggggtg
     2941 tgagtttagc cggataatac gtcggatccg gcttacatct ttatatcatt caatgctcac
     3001 ccgcgttacg ccatccgcct ccaccagact aaaccgcact ggcaagaaac gctccaccac
     3061 cgcaatattg gtcagcagat ggcatgaggg atgggcgacc gtaaattctc ccgcgcccgc
     3121 cagcgccatc ggcagtacca gctggtcggc gagatattcc cccaccgccg ccggacttgc
     3181 caggtagcgt ttcacctctt tcaccaactg tgccgcgacc acttcggcgc tgacgcgctt
     3241 ttcaccgacg acaaaaaagc gttcggtgat attttcactt tcgacttcaa gcgaaacggt
     3301 attacccggt ccctggtcac gcggcaggtt atgaatattc tgctcatgca gggaaaaact
     3361 ccccgccagt gtagcgattt cacgctcagc gacatggcgc ggcacgccag ctaatagcac
     3421 ctctccacgc atctgcacaa tgttcccgcg ctcgccaagt tgcaaggtgt taaacaatgc
     3481 caccggcgag acttccgttg ccaccacccc gcctccagca ggataaaaac cgtggcgtaa
     3541 taatgtggtt tgctgatgaa tgcccatttt cgccagcagc ggctccagca cccggcggat
     3601 aaaatccgca ggcggtgccg acgggttatc ggtgccgccg ctcacttcaa cacgcgaagg
     3661 cccatcagca aaccacagcg cgggtagcac cgtttgcagc accagcgtgc tgcttccggc
     3721 actaccgata gcaaagcggt aatcgccgcc acgcacggta ccgggccgga agaccagacg
     3781 ctgtgacccc agctccgcgc cttccaccgt tgccctacaa atttccgcag ccgcttttac
     3841 cgcggtcaga tgttggcgca acagccccgg tttcgcccgc ccggcacgaa tgccggtgat
     3901 ggtaaatggc tggccggtta tcatcgacag gctcagcgcc gagcgcagga tctgcccgcc
     3961 gccttcgccc tgtgcgccat ccagcgcaat catccttttc atcatttatc cttttacgca
     4021 caccacctga cgcagggtat agataacttc caccagatcg ctttgtgccg ccatcaccgc
     4081 atcaatatct ttatacgcca tcgggatttc gtcgatcact tcggcatctt tacggcattc
     4141 cacatgcgcg gtggcacgaa tttgatcttc cacgctgaac atttttttcg ctttagtacg
     4201 gctcataacc cgcccggcac cgtggctgca cgaacagaac gactcttcat ttcccagccc
     4261 acggacgata aagcttttcg ctcccatcga ccctggaata attccatatt gaccagcacg
     4321 cgcagacacc gcgcctttac gcgtcacgta gatctcttca ccaaagtgct gttctttttg
     4381 cacatagttg tggtgacagt tgatctcttc catcgccagc gtttgtggct gtctgaccgt
     4441 tttctgcgta atgctctgca acgccgttac cacgttttcc atcatcgcat cgcggttaag
     4501 gctggcaaaa agctgcgccc aggccacggc tttcaggtaa tcatcaaagt attccgtacc
     4561 ttccataaag tacgccagat cacgcgacgg caatgtttca agcgtttcct gcatctcttt
     4621 ttgcgccaga tcgataaagt aagtcccgat ggcgttacca attccgcgtg aaccggagtg
     4681 cagcataatc cacacctggt ccgactcatc aaggcagatt tcaataaagt ggttacccgt
     4741 tcccagcgtt cccaggtgtt tatagttgtt agtgttaagg aaacggggat atttttgcgt
     4801 taaccactga taaccggctt ccagctcagc ccatttcgca tcgacgttaa caggcggatt
     4861 ttcccatgcg cctttatcac gtttacaacg tccggtagta cgtccgtgcg gcacggctgt
     4921 ttcaatcgcc tgacgcagct ccgccaggtt ttccggcagg tctgccgccg ttaacgcggt
     4981 acgcagcgcg ttcattccac aaccgatatc cacgcccacc gccgccggaa taatcgcccc
     5041 tttggtcggg atcacgctac caatggtgga acctttaccc aggtgtacat ccggcattac
     5101 cgcaatatgt ttgaaaataa acggcatctt cgccgtatta ataagttgct gacgcgcatc
     5161 ggcctctacc ggcacgcctt tggtccacat ttttaccggg gcattttcag tggtcagtaa
     5221 ttcgtaattc attttgtttt tctcttttcg ttgt
//
LOCUS       DAZKGF010000250         5246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZKGF010000250 DAZKGF010000000
VERSION     DAZKGF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.250"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            698..868
                     /locus_tag="ACHOQV_004713"
                     /pseudo
     CDS             698..868
                     /locus_tag="ACHOQV_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1084..1917)
                     /locus_tag="ACHOQV_004714"
     CDS             complement(1084..1917)
                     /locus_tag="ACHOQV_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9632968.1"
                     /translation="MMKLGFNEATCLRNSSLEKDLVLAERFGYDYIEIRLDMLQTWLQ
                     EHTLVELADIFSVSHLKPWGYNSLEDITFCDAGRWSEIRQQLVLACYAGEVVGGDCLV
                     VVPTIREGAHFSDRDTVRDSVKRLREMSALAAESQMRLAFEPIGSAGCCVRSLAMAME
                     IVDAVDRSNVGLAIDAFNLYLYDQWQDLSTLRQVPVEKIFVYHIDDADRLPVAVLDHC
                     HRLFPGNGAIPLYMITHELVKKGYTGICSLELFNPGYWQMAASEVFAIGAEKTRPFLA
                     D"
     gene            complement(1924..3261)
                     /locus_tag="ACHOQV_004715"
     CDS             complement(1924..3261)
                     /locus_tag="ACHOQV_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HFP9632969.1"
                     /translation="MKGRLITSDPWNQQFMVELEVFFSPRQPACSELISVFPNHSLQQ
                     IDGFGGSFTESAGVVFNSMSDKTKAQFLNLYFSPQEHNYSLGRMPIQSCDFSLSNYAY
                     VDSSEDLRQGRISFSRDESHLMPLISAALRINPQIKLMASPWSPPAFMKTNYDMNGGG
                     KLRYEYYADWADIIIDYLLEYRRHGISVKALSVQNEPVAIKSWDSCLYSVEEETTFAV
                     HYLRPRLARYGMNEIEIYIWDHDKDGLIDWAERAFADEKDGDSINGMAFHWYTGDHFS
                     QIQYLARKLPNKKLLFSEGCVPMENDEGGQIRHWHTYIHDMIGNFKSGCSGFIDWNLL
                     LDSDGGPNHQSNLCEAPVQYDALNDILWCNHSWYGIGHFCRFVHPGAKVMLTSSYDTQ
                     LEDVGFVNPDGERVLVVYNRDTRERHFRVVDGDRELALTLPPSGASTLVWRQE"
     gene            complement(3359..4369)
                     /gene="iolG1"
                     /locus_tag="ACHOQV_004716"
     CDS             complement(3359..4369)
                     /gene="iolG1"
                     /locus_tag="ACHOQV_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase IolG1"
                     /protein_id="HFP9632970.1"
                     /translation="MTLKAGIVGIGKIGADHLRRLANTISGVEVVAVCDIVEGRAQAA
                     LDKYEIKAKDYKDYHDLINDEDVEVVVITASNEVHADVAVAALNANKYVFCEKPLAVT
                     ADDCQRVIGAERKNGKRMVQVGFMRRYDKGYVQLKNIIDSGEIGQPLMVHGRHYNVST
                     VSDYKTPQAIYETLIHEIDVMHWLLNEDYRTVKVYFPRQSSLVTTLRDPQVVVMETTS
                     GINIVVEVFVNCQYGYDIHCDVTCEKGMVELPTVATAAVRKEAKYSTDILVDWKQRFI
                     DAYDIEFQDFFDRLNAGLPLAGPTSWDGYLAAVTADACVKSQETGNTEFVELPAKPDF
                     YK"
     gene            complement(4387..>5246)
                     /gene="iolE"
                     /locus_tag="ACHOQV_004717"
     CDS             complement(4387..>5246)
                     /gene="iolE"
                     /locus_tag="ACHOQV_004717"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463285.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFP9632971.1"
                     /translation="WRNDDLPEIGKDNTYKQILSDAALTGFSGTEVGGCYPLDPTELN
                     IELALRHLEIPGKWFSSFIIRDGIESVKKAFEQHCAYLQAVHAYIAVVSEQTYSIQGI
                     IDKCVYTEKPYFNDIEWQQLCSGLNELGMIANTYGLKLAFHHHMGTGVQTLQEVDRLM
                     ENTDPQLVNLLFDTGHIYVSDGDVMSLLCKHFSRIKHVHFKDVRDEKLKACRAGKKSF
                     LNSFLDGMFTVPGDGNIDFKSVLAYLVENHYSGWIVVEAEQDPNIYNPLEYAQKGKKH
                     IDGLLGNYI"
BASE COUNT         1513 a         1302 c         1003 g         1428 t
ORIGIN      
        1 tttaactggt tgctgatata agtttttcac cacgcataaa cactgcccat gcgatcctgg
       61 caatcttatt tgccagctct atgatgacga tattcgttgg tcgacgtgcc tgctgggctt
      121 aaatctattc ccccggggat ccggctttat tgcaaccata aagctgcgag cccctatgta
      181 attcgctgac tcacacctca tttactgcgc gggattcaag cccaaaaccc catttaggtt
      241 tagtgagtcc ctgtcttacc tgtctttgtc atcacgttga ccaccagagc aacctttctg
      301 cattcaccca tttaagtaac caaaatgagt atcaggcctg atatatacag aactgaattc
      361 gttaattaat tattaatcag ttaaattttc cgttactggg atgaggtgta cacttggcct
      421 ctcccaaaat ccgcggaacc gcccctggcc cctattcagc atttcaggct ctaagttgag
      481 gactgaccgg attgacatag ggcctgctga cgccattcat tgactaccaa cagatctttc
      541 ttgttattta tagaaaacca ccagccagcc tagtggttca gtaaaacttc caactttgag
      601 gctgttgtca aaaccccttt ttgatttgtt aaaacacctt gcagtctacc aactgtaagc
      661 gtcaaacaag aaatcaaaag gggattccaa tgggtgaatg aaaaagggct taacgaacac
      721 ccgatggaac tgtaaatatc acatagtttt tgcgccaaaa taccgaagac agatgttcta
      781 cagagaaaag cgaagtgcaa gagcaccagg caatattttg agaaaactgt gtgagtggaa
      841 aagtgtacgg attctggaag ctgaatgctg agcggatcat atccatatgc ttgtgaattt
      901 acggggcgta agtaaggaag ttgaatgaaa atgtcagatc atatgcgcct gtcagccagg
      961 gcgaggcaga aaacgaatct ttaagacaca tctgaaaaac ctccggctat gccggaggag
     1021 atttattctg atcacctgct tattaccggg cacagcgaca gttcatttgc agttacgtca
     1081 gcgttaatcc gctaagaaag gtcgcgtttt ttccgctccg atagcaaaga cctcgctggc
     1141 tgccatttgc cagtaacctg ggttaaacaa ctccagtgaa cagatccctg tataaccttt
     1201 tttcaccagt tcatgggtga tcatgtacag tggtatcgca ccgttgcctg gaaacagacg
     1261 atgacaatga tccaacacag ctacaggtaa acggtcagca tcatcaatat gatagacaaa
     1321 gatcttttcc accggaacct gtcgcagcgt actcagatcc tgccattgat cgtacagata
     1381 aaggttaaag gcatctattg ccagaccaac gttactccga tcaactgcgt ccacgatctc
     1441 catcgccatc gccagactgc gtacgcaaca tcccgcagag ccgataggct caaaggccag
     1501 tcgcatctgg ctctctgccg ctaatgctga catctcacgc aaacgtttca ctgaatctct
     1561 taccgtgtca cgatctgaaa aatgagcacc ttcacgaatg gtcggcacta caaccaggca
     1621 atcaccacct acgacttccc ctgcataaca ggctaaaacc agttgttgcc gtatttccga
     1681 ccagcgcccg gcatcacaaa atgtgatgtc ttccagcgag ttgtagcccc atggcttaag
     1741 atggcttaca gaaaaaatat cggccagttc aaccagagtg tgttcctgca gccacgtctg
     1801 caacatatcc agacggatct caatatagtc atatccaaaa cgttccgcta ggaccagatc
     1861 cttctccagg ctggaattac gcagacaggt cgcttcattg aatcccagtt tcatcattca
     1921 gacttactcc tgccgccata ccaatgtact ggctccagaa ggcggcagcg ttaatgcgag
     1981 ttctctgtcg ccatcgacca cccggaaatg cctttctcgc gtatcgcgat tatataccac
     2041 taaaactcgc tcaccatcag gattaacaaa acccacatct tccagttgcg tatcataact
     2101 ggaagtaagc ataactttcg caccgggatg cacaaagcgg caaaagtgtc caattccgta
     2161 ccaagagtgg ttacaccaga gaatgtcatt caatgcatcg tactgcacag gagcttcgca
     2221 caaattactc tggtgatttg ggcctccatc actgtctaac agcaggttcc agtcgataaa
     2281 tccgctacag ccggatttaa aattaccaat catgtcatga atataggtat gccagtgacg
     2341 gatctggcca ccttcatcat tctccattgg tacacatcct tcggaaaaca gcagcttttt
     2401 attcggcaac ttccgggcca gatactgaat ttgcgaaaaa tggtcaccgg tataccagtg
     2461 gaaagccatc ccgttgatgc tatcgccatc cttttcgtca gcaaaggcac gttcagccca
     2521 gtcaatcaga ccatctttat catgatccca gatatagatc tctatttcat tcattccata
     2581 gcgggcgagt cgtgggcgca gatagtgaac agcaaaagta gtctcttctt ctacactata
     2641 cagacaggaa tcccagcttt ttatcgcaac tggttcattc tgcactgata gtgctttaac
     2701 actgatacca tggcgtctgt attcgagcag atagtcgata ataatatctg cccagtcagc
     2761 atagtattca taccgcaatt tcccacctcc gttcatatca taattagtct tcatgaatgc
     2821 cggtggactc cacggggaag ccattaattt tatttgtgga ttgatccgca atgccgcgga
     2881 aattagcggc attaaatgag attcatcacg ggaaaaggaa atccttccct gacgcaggtc
     2941 ttcactggaa tcaacatagg cataattgct cagagagaaa tcacagcttt ggatcggcat
     3001 ccgccccaaa ctatagttat gttcctgagg cgaaaaatag agattcaaaa attgcgcctt
     3061 tgtcttatca ctcatgctgt tgaaaacaac accagcactt tcagtgaagc tgccgccaaa
     3121 tccatcaatc tgttgtaacg agtggtttgg aaagacgctg attaattcac tacacgctgg
     3181 ttgacgaggc gaaaaaaaaa cctccagctc aaccataaat tgctgattcc agggatcgga
     3241 agtgattagt ctgcctttca tagagggatt ccagataata taggatgata aattgtgata
     3301 taccggacag gagcatacac ctagctatcc ggtatgaaat tatcacatcg cagaaagact
     3361 atttgtagaa gtcaggtttt gctggtaatt ccacaaactc ggtgtttcct gtttcctggg
     3421 acttcacaca ggcatcagcc gttaccgcag ccagataacc atcccatgat gttgggcctg
     3481 ccagaggtaa tcccgcattc agacggtcaa agaagtcctg gaactcaata tcataggcat
     3541 caataaagcg ttgtttccag tccaccaaaa tgtcagtact gtattttgcc tctttacgca
     3601 ccgcagcagt agccacggta ggtaattcca ccatcccttt ctcacaggtc acatcacaat
     3661 gaatatcata gccatactgg caattcacaa acacctcaac cactatatta ataccggagg
     3721 tcgtttccat caccaccacc tgcggatctc gtagagtagt caccaagctg gactgacgtg
     3781 ggaagtagac cttaactgtt ctataatctt cgttgagtaa ccagtgcata acatcgattt
     3841 catgaatcag cgtttcataa attgcttgtg gtgttttgta atccgatacc gtgcttacat
     3901 tataatgacg accatgaacc attaatggtt gaccaatttc tccgctatcg atgatgtttt
     3961 tcagctgtac gtagccttta tcgtagcgac gcataaaccc aacttgcacc attcgttttc
     4021 catttttacg ttcagctccg atcacacgct gacaatcatc tgctgtaacc gcaagcggct
     4081 tttcacagaa aacatattta ttggcattta acgctgcaac ggctacatcg gcatgtactt
     4141 cattcgaggc agtaattacc actacttcga catcttcatc attaatcaga tcgtgatagt
     4201 ctttatagtc ttttgctttg atctcatact tatccaaagc tgcttgcgct ctgccttcga
     4261 caatatcgca cacagcaaca acttctactc cagaaatagt gttagccaga cgtcttaagt
     4321 gatcggcacc aattttcccg atccccacaa tacctgcttt taaagtcatc ttattttcct
     4381 tttctatcag atataattac ccaataaacc atcaatatgc tttttacctt tttgtgcata
     4441 ttccaaagga ttatatatat tcgggtcctg ctcagcttca acgacaatcc agccggaata
     4501 atgattctca accaaataag ccaatacaga tttaaaatca atattcccat caccaggaac
     4561 agtaaacatt ccatcaagaa aagaattgag aaatgatttt tttccagccc gacatgcttt
     4621 tagtttttca tcccgaacat ctttaaagtg aacatgttta atacgggaaa aatgcttgca
     4681 caacagtgac atcacatctc catcagaaac ataaatatga ccagtatcaa acagcaaatt
     4741 cactaattgt ggatctgtgt tttccataag tcgatccact tcctgtaaag tttgtacgcc
     4801 agtgcccata tgatggtgga aagcaagttt aagcccatat gtattggcga tcatgcctaa
     4861 ctcgttcaat cctgagcata attgttgcca ttcgatatca ttgaagtaag gtttttccgt
     4921 atagacacat ttatcaataa ttccttgaat actatacgtt tgctcagaca caacagcgat
     4981 ataagcatgg acagcctgca aataagcgca atgttgttca aaagctttct tcactgattc
     5041 aataccatca cgaataataa aagatgagaa ccactttccg ggaatttcca gatggcgaag
     5101 tgctaactct atatttaatt cagtcgggtc cagaggataa catcccccca cttctgttcc
     5161 agaaaaacca gtaagtgctg catcacttaa tatctgctta taggtattgt ctttaccaat
     5221 ttcaggcaaa tcatcatttc gccatc
//
LOCUS       DAZKGF010000251         5133 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZKGF010000251 DAZKGF010000000
VERSION     DAZKGF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.251"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(42..275)
                     /locus_tag="ACHOQV_004718"
     CDS             complement(42..275)
                     /locus_tag="ACHOQV_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632972.1"
                     /translation="MARSDYDFINLSLEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALNAAYSEHPEWFSGLVSGDEN"
     gene            complement(370..993)
                     /locus_tag="ACHOQV_004719"
     CDS             complement(370..993)
                     /locus_tag="ACHOQV_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9632973.1"
                     /translation="MHTRIDYLADKYCFTELNESSRLRLQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKHFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRMVGSENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRKAGMPVVTTSVEVHDNLTGTTRKIPAYHL"
     gene            complement(1082..1591)
                     /locus_tag="ACHOQV_004720"
     CDS             complement(1082..1591)
                     /locus_tag="ACHOQV_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632974.1"
                     /translation="MQITEALLSEPGEIRRFVQQAVDHWPHLLAIHFTLHSTEGVCGQ
                     QIQTFCSSFHRRVHERITERDHTVSPSAPVVLRWLREQHEGAPIRCLLLLSQTSVCHP
                     RASVTVDEACSQVADLLQQTWPVISAGGQCRVERCFRVVRPDTSEQYVALKTAVQSLM
                     PRGIATIIR"
     gene            complement(1872..2050)
                     /locus_tag="ACHOQV_004721"
                     /pseudo
     CDS             complement(1872..2050)
                     /locus_tag="ACHOQV_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
     gene            complement(2118..2391)
                     /locus_tag="ACHOQV_004722"
                     /pseudo
     CDS             complement(2118..2391)
                     /locus_tag="ACHOQV_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2438..3004)
                     /locus_tag="ACHOQV_004723"
     CDS             complement(2438..3004)
                     /locus_tag="ACHOQV_004723"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP9632975.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDKSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGAVERHLGCSDF"
     gene            complement(3032..3229)
                     /locus_tag="ACHOQV_004724"
     CDS             complement(3032..3229)
                     /locus_tag="ACHOQV_004724"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP9632976.1"
                     /translation="MPQDDFCIVNPGGCIIHFPANGRYILDIHDPDFPEQYQRLLERL
                     DYLTKPGTKASGQRNSGYSGF"
     gene            complement(3477..3902)
                     /locus_tag="ACHOQV_004725"
     CDS             complement(3477..3902)
                     /locus_tag="ACHOQV_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP9632977.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCRGRLILQTD
                     AQGAWFEHDSHALSAQQKATCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRATCTKSVYRYQPDLCQDCGGAST"
     gene            complement(3899..4282)
                     /locus_tag="ACHOQV_004726"
     CDS             complement(3899..4282)
                     /locus_tag="ACHOQV_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP9632978.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWRCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
     gene            complement(4654..>5133)
                     /locus_tag="ACHOQV_004727"
     CDS             complement(4654..>5133)
                     /locus_tag="ACHOQV_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632979.1"
                     /translation="FAYRKDTPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTP
                     DKGLHIHFIGYLDGQRHKNSYQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVI
                     HYSDKSAVDDLRYALSYLAKQDQKEHGIILGRSRLPEKSNRGRPRLDSTLSEICSLV"
BASE COUNT         1303 a         1362 c         1221 g         1247 t
ORIGIN      
        1 tgccggcaat cacactgcct gatggcagca taatccactt attaattctc atccccggag
       61 acaagccctg aaaaccactc gggatgttca ctgtatgctg cgttcagagc atcccaggaa
      121 acgttcatac agaagctgtc ctgcaatttg caggcaacca cctctgccag gcggttccgg
      181 ttatccgcca gtccggcata acctctctct gccagccact cattcagttc atgttccaga
      241 gacagattga taaaatcgta atcggacctt gccattcccg tttccttaac acatgtcttc
      301 agactcaggg ataaagtata aaaatgtcgc cctgctgctg ccagagcaat attccccctg
      361 atggccgtat cagagatggt atgccggtat tttacgggtg gttcccgtca ggttatcgtg
      421 tacctccacg ctcgtagtca ccaccggcat tccggccttt ctcagcctca aaacatcagc
      481 tgcaatacgc tgactaccag accagaacag gccgtccagt gcagtcacca gtaaccccgc
      541 ctccagcgca tacttcagcc gttcacgggg cgctttcact tcccgggcaa cctgctgata
      601 tggcaatgat gtgttttcac tcccgaccat ccggcgaata cgatgagaca gatgataccg
      661 gtatgtatcc ggcacacctg aaaggctggc cttcaggctg tacacgcagc caaagtgttt
      721 atcgttgaac accacatttt tctggctgat atcccattct tcacgcagcg cggcaatcag
      781 ttgtggtgta cgggtaagca acaagcgaaa aggcagttca aaactggtga cgtaatccac
      841 attcagcagg gcaatgcgaa gtcgttcttc tggtccggct tctgtctgcc ggcactcctc
      901 caggacatcc tgccactgca ggcgaagacg ggaagactca ttcagttctg taaagcagta
      961 tttatccgcc agatagtcaa ttcgtgtatg catactgaag agtattccgt ataaagattc
     1021 agctggcaaa actttatcag tctgtaaaaa ctaacggaag agtcgatatt tctcccgtcc
     1081 atcaccggat gattgttgca ataccccgtg gcatcagaga ctgaacagcc gtttttaacg
     1141 caacgtattg ctctgatgta tcaggccgga caacccgaaa acacctttcc acccggcatt
     1201 gtccgccagc gcttatcacc ggccaggtct gttgcagtaa atccgccact tgcgaacatg
     1261 cttcatcaac tgtgacactg gcccgcggat ggcaaacact cgtctggctg agcagcaaca
     1321 gacatcgtat cggtgctcct tcatgttgtt cccgcaacca gcgtaatacc accggcgctg
     1381 atgggctgac cgtgtgatca cgctccgtaa tacgttcgtg cacccgtcgg tgaaaggaac
     1441 tacagaatgt ctgaatctgt tgaccacaga cgccttctgt cgaatgaagt gtgaagtgga
     1501 ttgccagcag atgcggccag tgatccaccg cctgctgaac aaaacgccgg atttcccccg
     1561 gctctgaaag taaggcttcg gttatttgca ctattttatc tctgttgaac ttggttaagt
     1621 cggtgcagac gcatcaacac aagtacggtt cgatgcaaac agctgtgact ggcaatatga
     1681 aaggaatgat gaatcagtca ggatgacaaa gtgccagctg accggagggg acgcaggaag
     1741 attcacgggg ggaccagcac cagggaacag cgccacaata ccaacgctga cacgttgaac
     1801 attgccagcg taccggtatc acaacacgtt tcatacttct gccaccgtga ttcttcgatt
     1861 cgttactgta tctactgtga cacttcgctt ttatacctgc ggctggatcg gcccggcttg
     1921 atgaatcttc actgatcagc ttataaaccc ctctgtcggt cataccggtg aaactggtga
     1981 tatagttcat gtcaatcagg gaattatcgg cacgcagaaa tacgctgtcg tggcttgttg
     2041 tagtcaacat ggtcagaatg tcctctgtga gatttatgaa gattgtgcga atgcggggaa
     2101 tctgctgagc tgtgctttca gaactggcct gttacgggat accagggatt accggcgggg
     2161 taacgggctt ccggatcata cacaccacga ttatcgcgga caaaatcact gaacgcccat
     2221 atcacctctt taagtatgtc ttcgcagtcc ggtacatgac gatccagcgc cacatcccgg
     2281 gtggtactac tttgatgcgc ccggtgacac aaagcccgga ttgttccaga catcctgaat
     2341 caaacgcccc agattagggg cgtcgaaata tgcctctctg accattatat tccggcgtac
     2401 aggtagcagg tcagaagtga caatgcgtca cctgacgtta aaagtcacta cacccaagat
     2461 gacgttcaac agcaccatgc gattcaatgt aagcccgggc tgtctgttcc agtacaccag
     2521 gctcagcgtt gtatgtgtta gctgcatcaa ataccaacga cagcacttca ggatacacaa
     2581 ccagatgtgt aatggagtta tcttcaccca atacttttcc ccacgcctgc tcaatcagat
     2641 ttctgagaac caccacctca cgactcttac accagacatc gttattaagt agcagcacca
     2701 taagataagg agtggtatcg ttagccacag cctccctact ccagagataa tataaagggg
     2761 tgggctcaac agatttatct ttacgtcgct tacactgcaa atattcagaa atgagtctat
     2821 gcagttcacc agtaaaatca gccatcaaag agggaatggc cttattaata ccctgccctg
     2881 gtattaattt aaattgtaat aatttaattt caggatgtgt ggctgcagcc cgatacagag
     2941 ttgcaaggac acacttttgc cagagggcgt tacaggaaag cttaacgttt gattctgtat
     3001 acataataaa tcaccttaca gttacaacag gtcaaaaacc gctgtagcca gagttacgct
     3061 ggcctgatgc tttagtaccg ggcttcgtca gataatccag acgctccaat aagcgctgat
     3121 actgctcagg gaaatcagga tcatgaatat ccaggatgta acgtccatta gcagggaaat
     3181 gaataatgca gccccctgga ttaacaatgc agaaatcgtc ctgaggcact gatcaatacg
     3241 gagaggactc tcgcgtgtgg tttattgaca ccacagtgca gattcggcga atccgcgatc
     3301 acggtgcaat ttcgttccac agcacacaat cataaccccg ggttttattc aggtaagcag
     3361 gactgcggat atccggtgtc gcgcctttgt gtcacgaacg gggtaggtgc gaaacaccgg
     3421 ataaaatgca ggctggcaat acctctgaac gccctgcgca gagcggatat tttggattaa
     3481 gtactcgcac ctccgcagtc ctggcacaaa tctggctggt acctgtaaac agacttcgta
     3541 catgttgctc tgcaataaat ccccgtacca caggcctcac agaatttctt cccatgaaaa
     3601 aactgctcac acatcacaca atgccactcc agcacgaccg gtaatggcga tagaaacatc
     3661 gccatatcct caatgtaagg gtgggacttt tccggattca gcaccacgca ggtcgccttc
     3721 tgttgcgcgc tcagggcatg tgaatcatgc tcaaaccacg ccccctgagc atctgtctgc
     3781 aaaatcaacc gaccacgaca ggaaaggcag aaacactgcc ggatatttct gctgagcgca
     3841 gagtcggcac tgataatgtg ttcacccggc gtgatcccca gccccgtttt tataccgttc
     3901 attcagccac tccctcctca ctgaagtgcc ctgtatggca gtgtgtgcag taccgctccc
     3961 cataataatc gtggtgacat tgtctgcagc gccagctggc tttacgcacc acgggtaagg
     4021 catccgggac gaattgctgc agacgtttaa tcaaccgtat ttctctgcgt tccggcctga
     4081 cataaggaca ttgctgcgcg tgctccgtca gcccttcgtc tgcgtgttca aaccagggac
     4141 gctcagtgtc gtattgcggg tggtatctga gcgcactgcc gcaaaggtgg caagtgtagc
     4201 gatcgtaagg tgcagtctgt gcggtacggg cacccgtcag acgcccgttg ccatcaaatg
     4261 caataaagga ttttgcgtac atcgtcatat tcctcagccg ccagacgaca cgcaggcaac
     4321 agctgtcccg gtacgggcag tgtggtcagg gtgtgaatgg cggttcagtt aaaatctgga
     4381 ctggagtttg ggtaaaacag gctggttgct gttaccgcac tgtggttgtc acacaatggc
     4441 ccacctgcgt aaaaaactgt cagcaggcag ggatggacat cgggggatgt gctgtatcgg
     4501 ttgccggaag gaatggtaaa gaatgtgcca tggttaaatc tccgtatggt tcatacggaa
     4561 tagccatgtc gtaaaaaatg cgcagagtcc ctgaagtggc ctccggaacc gactgtaaca
     4621 attagcgcca gcccgtagtt gtcgttcagg ggcttacacc agagaacata tctccgacag
     4681 ggtactgtcc agtcgcggtc ggccccggtt acttttttcg ggaagcctgc tacgccccag
     4741 aataataccg tgttctttct gatcctgttt agccaggtag ctgagggcgt aacgtaagtc
     4801 gtctaccgca gatttatcac tatagtgaat cacgtgatcg atacgcaccg ggtatttgtc
     4861 tttagcccgg cacagatgaa aataaccctc cccttccgtg atccgcctcc agatatctcc
     4921 cagttgcctg gatatctgat aagagttctt gtgacgttgt ccatccagat agccgataaa
     4981 atgaatatga agccctttgt ctggtgtata ttccattacc cagtaatatc ctgctaacat
     5041 cgtctgggtt tcgctaagta aacggtatat ttccatacac atactgtgct tacaggaatg
     5101 cccgaaactg ggcgtgtctt tcctgtaggc aaa
//
LOCUS       DAZKGF010000252         5116 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZKGF010000252 DAZKGF010000000
VERSION     DAZKGF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.252"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(343..966)
                     /locus_tag="ACHOQV_004728"
     CDS             complement(343..966)
                     /locus_tag="ACHOQV_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9632980.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRESGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKAPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
     gene            1115..1600
                     /locus_tag="ACHOQV_004729"
     CDS             1115..1600
                     /locus_tag="ACHOQV_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFP9632981.1"
                     /translation="MIKYIFALTLLLSGSVCADQIQGLVIRVLDGDTLEILQARHPVR
                     IRLVNIDAPEKKQPFGRWSMNQLKSLVVNTPVTVTYTQTDRYGRILGRVYTEDGMEVN
                     RWLVQHGAAWVYPDFNTDYTLPAYQREARTMKRGLWADKKPVPPWLWRKENKNSHNNY
                     K"
     gene            2244..2608
                     /locus_tag="ACHOQV_004730"
                     /pseudo
     CDS             2244..2608
                     /locus_tag="ACHOQV_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2618..2809
                     /locus_tag="ACHOQV_004731"
     CDS             2618..2809
                     /locus_tag="ACHOQV_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632982.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPGGE"
     gene            2843..3166
                     /locus_tag="ACHOQV_004732"
     CDS             2843..3166
                     /locus_tag="ACHOQV_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFP9632983.1"
                     /translation="MAYHSPDITLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRMREQMSGIIRKTGTDEILFRLSKHHHLYEKK
                     KTKEG"
     gene            complement(3309..3404)
                     /gene="dqlB"
                     /locus_tag="ACHOQV_004733"
     CDS             complement(3309..3404)
                     /gene="dqlB"
                     /locus_tag="ACHOQV_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFP9632984.1"
                     /translation="MINLLIIVLRAVVAVANAVIAILELIRELID"
     gene            3903..>5116
                     /locus_tag="ACHOQV_004734"
     CDS             3903..>5116
                     /locus_tag="ACHOQV_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFP9632985.1"
                     /translation="MSYQHSPTTEDPMNIWYIVGTICLLFAFLIWLFLPDIVFATCLI
                     LHTMWGMTDWGPFHHFAAPRYNLLANTANNAATITFSQWLDVMSQTVGILWLILLPMT
                     LGFLWMWFQHPAQPRFTRRPLNIHTLPHLFSSLSPAIAPVLADGDSNRLFHGQKRPER
                     RVALTPEVFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTG"
BASE COUNT         1380 a         1377 c         1156 g         1203 t
ORIGIN      
        1 aactcttttc cgtttaaaga aagaataaat gaagaacaaa cataaaaccg ccagaaaaag
       61 aaatgtagct atagatatta taaaatactc cgtcacctgt gaattctcat ccagagatga
      121 atacagcgaa taatatgtgg ttgtgagatt aagccttgtt atcaatatat aaataactga
      181 taatagtata atataaagaa tcagaccaaa tctgtttttc tgcgctccgg ttaatacttc
      241 tgatttatct cgcataaaat atcctgtctg taatctgtcc cggtaaacaa tggtaaattt
      301 cagaatcagt gcgaatacgg taacaaagat tcctcctgtg ctttaaagtg tgtaggcaga
      361 aaccagtctg gtggttccgg tcagggagtc tgatacctca acttcccggg tgactataac
      421 catacctgac tgacgcagtc tctgcacatc agctgcaatt cgctgagttc ccagccagaa
      481 caggccatcc agtgccgtta ccagcaaccc gctttccagc gccagtttca gccgtgccgt
      541 cggggcttta acctgcccgg cgatatcagt aaaacaacgc gtcgtcatgc catagcagtc
      601 catccgccgt attcgcgtcg caagactgta gctgaaacaa tccggcacgc cggataagtc
      661 attctgcaga ctgtatacac atccccgctg ccgcccgttg aacagggctt ttttctgact
      721 gagcagccat tcccgacgca gagtgtcaat catctgcggt gtccgggtta acagcagacg
      781 aaacggcagc tcaaaactgg tcacataatc aacattcagt aatgctgtcc gcagtcgctc
      841 cctggcacct gccccgctct cccggcattc acgcatgaca tcagcccact gacgcatcag
      901 tcgtggagac tcattcctgg ccctgaactg atatttctca atatgataat caatacgttc
      961 ctgcatatcg ccaccggata tctgttgttt acccagacac agtgattagc ctacaggaat
     1021 aaatatcctc cgtgaagcca ggtgcctcag aagataaaaa catgtacttt tttcaacgtg
     1081 taatattaac accacctcca caggaaaata taaaatgata aaatatatat tcgcactcac
     1141 acttctgctt tccgggtctg tctgtgccga tcaaattcag ggactcgtta ttcgtgtgct
     1201 ggacggagat actctggaga tattacaggc ccggcatcct gtgaggattc gtcttgttaa
     1261 tattgatgca ccggaaaaga aacagccttt tgggcgctgg tcaatgaatc aacttaaaag
     1321 ccttgtggta aatacgcccg taaccgtgac atacacacag acagaccgct acgggcgtat
     1381 tctgggacgg gtatataccg aagacggcat ggaagttaac cgctggctgg tacagcatgg
     1441 tgcagcctgg gtatatcctg atttcaacac agattacacc ctgccggcat accagagaga
     1501 agccagaaca atgaaacggg gtctgtgggc agacaaaaaa cctgttccac catggctgtg
     1561 gcgaaaagaa aataaaaata gccacaataa ttataaataa gacatctcac agaaatatat
     1621 ttcctgccgt tttatttccc ctgccagtcc ccaacgggaa ataacccaca gaaattacac
     1681 agatgtgaat aataaacctg ttcgatgtat ccgtcacaaa agagttgatg tttgactcag
     1741 aagaaaatac gcctgaagtt aaggaaggct tcagcttcat gattgttctg acatgaacat
     1801 atatccctct gtagcgccct gcctttcata acatttctat tacagcaaca acgaaagaca
     1861 acagcaatgt cggcctttta cgtccacatg aatattccac ttatccggaa ttgtgacaca
     1921 gcccctgaat acaggattga ttgccagtgc ccacaaatgg cacattttca gtaagattct
     1981 gaatgttaca ttcccccgtc atgtgtggca caactgtcac cgggaaagaa acggaaagca
     2041 catgaaaaac atcaaaactc acaccggact ccttatccac aaagaacaaa cgcggcgcgt
     2101 tcgtcttcat gaaactccgg ccgcctggtg ccacacgcac cgggaatgct acagcaaaac
     2161 gaccggtcgt cgctgcggca gccctgagag tctcagcagg ctgattctga gcagcattca
     2221 gcccatagaa tgagatgacc cgtatgaatg atggcagaca gacgcctccg tcccctgagg
     2281 catggaaaat ccggtttctg gcgaacggta cacccggcac atggttttac acggatgagc
     2341 agccgtcaca aaagagtggc tgcgggcaca ttgtgacgat gtggaagtga tccccctgtg
     2401 ccgtatgcat gacggtcccg ttttccggga acaccatatt aatgagctca gcgccgcact
     2461 ggccagaatg ctccatatgc accacatact gctggaacga acccggtaca gagaaaatat
     2521 ccatgatgca gaatgtattc gtgaaattca cgaagctccc ctgcaggcta tcggcatcct
     2581 ggaaaaatac aaacgcacaa aatcataagg cagttctatg tggatactga ttttctggct
     2641 ctcatccccc tttaatgtct cctccggcga tgcccggcaa cccacactgg ccgttcacag
     2701 tcaggagttc cgtactgaaa aagcctgtcg ggatgccttc accgcgctga aaaaagaaaa
     2761 cgtgggagaa ttcgctctcc ggggcgtctg cgtccccggc ggagaataaa ccggagatga
     2821 tgattagcca caggagtatt caatggcata ccattccccg gatatcacac tgcattatcc
     2881 gtatctgcgc cggatgtcct gggcgcagat aacggcagca gctgaagatg cggaaataca
     2941 tcatgattat gcccgggcat taatattgtg gcagcatgca tatcatgctg ccacgctgac
     3001 cctcaacaaa aacctggcag ttgctaaaat cgacttctgt attaaacgta tccggatgcg
     3061 tgaacaaatg agcgggataa tccggaagac tggcacagac gaaatactct tccgtctgag
     3121 taagcaccat catttatatg agaaaaagaa aacaaaagag ggatgacaat cccatgatat
     3181 gggaaaaact tgccatgctt actgcaggtg catttttgat actggcagtc ctgtgggggt
     3241 aataatatcc tgtttattaa agggcagaag gcggtgcccc tctgccctta agtacgtttc
     3301 cgggattatc agtcaataag ttcccggatc agttccagga ttgctatcac tgcgttagcc
     3361 acagcaacaa ccgccctgag aacgatgatt aacaggttaa tcatccgttt ctccgtcaat
     3421 ggaggaaatc actgttgttt actcttacat tgcctgtcac aacagctctt tcattgttag
     3481 atgtaaagtg atttctctcc agccagagca ctgaacacag caccacctgt tcgatcagcc
     3541 aggacttaaa atctcgcgtt catcgtccca ttggagatca cggcagaagc atcaccagac
     3601 agagctaacg gagcgattat agtcattttt ctgattgcaa aaaagagaag cgcagcctac
     3661 tatgatggtg ttagatgtag agtgatttcc ctttcggtca gagtcaccaa ctccgccccg
     3721 gaatagcaaa aaaaccgacc tcaccagtcg gtttttttgt gcctgtctct cactgccttc
     3781 tgctgttcct gtgaaaatac cctggttatt tcatccctgc ccggtcatta ccggcgattg
     3841 ttcgcattaa ttccactcag cctcatcccg aaatttatcc atttatcgat ggaggaatac
     3901 tcgtgtctta ccaacactca cccacaactg aagacccgat gaatatatgg tatatcgttg
     3961 gcaccatatg cctgctcttt gcgtttctca tctggctctt tttacccgac atcgtctttg
     4021 ccacctgcct gatcctgcac acaatgtggg ggatgactga ctgggggcct tttcaccatt
     4081 ttgccgcccc ccgttataac ctgctggcca acaccgccaa caacgctgca acgatcactt
     4141 tcagccagtg gctggatgtc atgtcacaga ccgtggggat cctctggctg attctgcttc
     4201 ccatgacgct cggctttctg tggatgtggt ttcagcatcc ggcccagccc cgcttcaccc
     4261 gcagaccact gaatatccac acgctgccac acctgttttc atcactgtca ccggcgatcg
     4321 cccccgttct ggctgacgga gacagcaaca gactcttcca cggacaaaaa cgtcccgagc
     4381 gccgtgtcgc actaaccccc gaagtctttg tggagcagaa caaccttatc cgcaacatgc
     4441 agctcgatgt tgcgtccaca cgccagtgct tcatggcgca actggggcaa ccactgacca
     4501 gctggaaaga catggcccca catgaaaaag cgctctttgc catctttggc ctgcagttct
     4561 tcctcggtga ccggaaagca gcggtagccc tgatgaacaa cctcaatctc tcctgcaggc
     4621 tcaaaagtaa acgggaccag gggcgtttca gcacgccggt gtattcactg gccagaaacg
     4681 cctttatccg ggtcatcaaa acagaaggcg cacaacagtg gctcaggcag caccgttacg
     4741 ttcgcagcgg tctggtctgg ctttacgcac atgatttgcg cctgacgcct ccgaactggc
     4801 tgtggctcaa gggcgttgac cggacgctct tctatgccct tcaccgggca aacaccacaa
     4861 aaggcttcat cgaaggggcc ggcgtggtgg ccgttgcccg ggcagaaaat gaagccagcc
     4921 gtctcggcct cccctgcccg gaaccctgtg tggaagaggc cattgaaggg cttcgacggg
     4981 acatgctggg gctgggcctg atatgggacg aacctcagcc agatcgcgat cgtaaacgtc
     5041 agatcaggac ccgctggtca ctcacggatg acgtgatacc ccgccgccat gataacgacg
     5101 aagacactga cactgg
//
LOCUS       DAZKGF010000253         5087 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZKGF010000253 DAZKGF010000000
VERSION     DAZKGF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.253"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(291..1250)
                     /gene="nrdF"
                     /locus_tag="ACHOQV_004735"
     CDS             complement(291..1250)
                     /gene="nrdF"
                     /locus_tag="ACHOQV_004735"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9632986.1"
                     /translation="MKLSRISAINWNKITDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1260..3404)
                     /gene="nrdE"
                     /locus_tag="ACHOQV_004736"
     CDS             complement(1260..3404)
                     /gene="nrdE"
                     /locus_tag="ACHOQV_004736"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9632987.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSSDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDIERVYGKPFADVAISEHYAEL
                     VANERIRKKYINARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSSITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3377..3787)
                     /gene="nrdI"
                     /locus_tag="ACHOQV_004737"
     CDS             complement(3377..3787)
                     /gene="nrdI"
                     /locus_tag="ACHOQV_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9632988.1"
                     /translation="MSQIVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3784..4029)
                     /gene="nrdH"
                     /locus_tag="ACHOQV_004738"
     CDS             complement(3784..4029)
                     /gene="nrdH"
                     /locus_tag="ACHOQV_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP9632989.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAANA"
     gene            complement(4276..4605)
                     /locus_tag="ACHOQV_004739"
     CDS             complement(4276..4605)
                     /locus_tag="ACHOQV_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFP9632990.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4757..>5087
                     /locus_tag="ACHOQV_004740"
     CDS             4757..>5087
                     /locus_tag="ACHOQV_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFP9632991.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLF"
BASE COUNT         1249 a         1250 c         1287 g         1301 t
ORIGIN      
        1 tctaccctaa catactgaga atctgaggca acccctgatg gcaaatgtgg catgaatatt
       61 gatgtgagca aatcgccgga atttcccatg atataagggc tgagagcaaa tcgagaaaag
      121 ttgatatttt ttgtcaaaga tagcaaaaaa acgcctgaat attgtggttt caggcgaata
      181 atgcaacaga gtatcagtgt agatcaccac aaattatttg cgtgatataa agctatttgg
      241 cgggaattat ttccctgcag cgggtagtga tatttttgaa aataaccccc tcagaaattc
      301 cagtcttcat cttctgtttc gaccgctttt cccatcacat atgaagagcc tgaaccggaa
      361 aagaaatcgt gattttcatc ggcattcggc gaaagcgcgg cgaggattgc cggattcact
      421 tccgccattt ctgcgggaaa tagcggctcg tagcccagat tcatcaacgc cttattggcg
      481 ttgtaacaga gaaacgcttt cacatcgtca gcccacgggg tttcggcgta cagctcatcg
      541 gtgtattgca actcgttgtc atagagttcc agcaacaaat cgaaggcgaa actcttcaat
      601 tcttcacgtc gtgcctggga gatcttttcc aggtttttct gatatttata gccgatgtag
      661 taaccgtgga ctgcttcatc gcggataatc agacggatca ggtctgcggt attggttagc
      721 tttccgcggc tggaaaaata catcggcagc cagaaaccgg aatagaacaa aaaagattca
      781 agaaacacac tggcgatttt ctttttcagc ggatcatcac cgcgataatg ttgctgaata
      841 atctgtgctt ttcgctgcaa cggcgcgttt tcttcactcc aggcgtaggc ggcatcgaca
      901 tctttggtct ggcacagcgt cgagaaaatc gaactgtaag agcgggcatg aaccgcctcc
      961 ataaagctga tattcgataa tactgcctct tcatgaggcg tgagcgcatc gggcatcagc
     1021 gaaggcgcgc cgataacatt ttgcagcgtg tcgagcagtg tcaggccagt aaaaacgcgc
     1081 atcgtcagtt gttgttctac gacacttaat gtctgccagg cgggaatatc gttcgatagt
     1141 ggcacctttt ctggtagcca gaaattgctg gtcaggcggt tccacacctc cagatcttta
     1201 tcgtctgtta tcttgttcca gttgatggcg ctgatacgtg agagtttcat agatattcct
     1261 taaagtgcac aggagacgca gccttcaatt tcagtgcctt ccagcgccat ctgacgcagg
     1321 cggatgtagt agagcgtttt gatacctttg cgccaggcgt aaatctgcgc tttgttgata
     1381 tcgcgagttg tggcggtatc ggggaaaaac agtgtcagcg acagcccctg atcgacatgg
     1441 cgagtcgctt cggcgtaggt gtcgatgatc ttttctgcgc ctatttcgta agcgtcctga
     1501 tacagcgcca gattctcgtt agtcataaac ggggcaggat agtaaacgcg tcctgttttg
     1561 ccctctttgc gtatctctac tttcgccaca atcggatgaa tactcgacgt ggcatggttg
     1621 atataagaga tagaaccggt tggcggcacc gcctggagat tttggttgta tatgccatag
     1681 cgcatcacgt cgtcgcgcag ttgcgcccac atttcgcggg taggtaacgt gatgctgcta
     1741 cgagcaaaca actcgccaac tttcgccgtt ttcggttgcc agttactttg cagatattgg
     1801 ctaaaatatt caccgctggc atagcgcgac tgtttgaacc cggcaaaggt ttcgccgcgt
     1861 tcgcgcgcca gcaacatcga ggtacgcagt gcgtgccagg tgatggtata gaaatagaga
     1921 ttggtgaaat cgagtgcttc cggcgatcca taagcgatgc cttctcgcgc cagataaccg
     1981 tgtaaattca tctgccccag tccgatggcg tgtgaggcgg catttccggc ttcgatggac
     2041 ggcacgctgc ggatatgact catatctgac accgccgtta aaccgcgcac ggcagtctct
     2101 accgtgcggg caaagtcggg ggaatccatg gtgtgggcaa tattcaacga acctaaattg
     2161 caggaaatat catggcctgt gcgggcatag tcgagattct cgtcatactc tgaggcgctg
     2221 ttaacctgca aaatttctga gcagagattg ctcatattta tgcgcccggc gatagggtta
     2281 gcacggttaa ccgtgtcttc atacatgatg tacggatagc cggactcaaa ctggatttct
     2341 gccagtcgct ggaagaaatc acgggcgttg atgtattttt tgcggatgcg ttcattggca
     2401 accagttcgg catagtgttc actgatggcg acatcggcaa atggcttgcc ataaactcgc
     2461 tctatgtcat agggcgaaaa cagcgccatc tgcgcattct cttttgccag atggaaagtg
     2521 atatccggga tcaccacgcc cagcgacagt gttttaatgc ggattttttc gtcggcattt
     2581 tcccgttttg tatcgagaaa acgcagaata tcgggatgat gagcatgtaa atagactgcg
     2641 ccagcaccct gacgcgcgcc gagctggttg gcataggaaa atgcgtcttc cagcattttc
     2701 atcaccggaa tcacgccaga agattgattt tcaatacgtt taattggcgc gcccgcttcc
     2761 cgcagattcg acagcaaaaa tgcaacgccg ccgccgcgtt tcgacagttg cagtgcggaa
     2821 tttactgccc ggccaatcga ctccatattg tcttcaatac gcagcaaaaa acaggaaacc
     2881 agttcgccgc gctgctgctt accacagttg aggaatgtcg gtgttgcagg ctgaaagcgt
     2941 cctgacagca tctcatcggt cagttgcgtc gccagtgttt catcgccttg tgccagcgtc
     3001 agcgccacca tcgtgactcg atcggcaaaa tcttccagat aacgcttacc gtcgaatgtc
     3061 ttcaacgtat agctggtgta aaacttccat gctccgagga atgtctgaaa acgaaaaccg
     3121 ctggcgtgtg cgtgggcaaa cagcgtaatg acaaaatcgc tagagtagcg attaaggaca
     3181 ctttcatcat agtaaccttc gttaaccagc cagttcaggc gctgctgctg gctactgaag
     3241 gccacactgt tcggacgcac atgcgtcgtc ataaaggcgt ctactgcctg gcgatcttta
     3301 tcgaactgaa tgcgacctgc gctatcgtaa aggttaagca tcgcattcag cgcgtggtaa
     3361 tccatagttt cctgcgtcag gcgttctgcg gttgtcgttg ccaaaattcg gttactcctt
     3421 tacgaacgtt ttcgatatcg ctttgcgtac ccatgagttc aaagcggtac agccatggca
     3481 cgccgcattt ccgggcaatc acatctccgg cgcggccata tgcctcacca aagttgcgat
     3541 tacccgaagc aataacgccg cgcagcaacg cccggttatg ctcgtcgttt aaaaagcgaa
     3601 tgacctgtcg tggcaccgcg ccagccgtac cgccgccgcc gtaagagggc acgatcagga
     3661 tgtaaggctc gtctacctga atccgttccc gctcattgag cgggatgcgc accgcgggca
     3721 gccctaaacg ttcgataaaa cgctgcgtat tttcggagct gctggaaaag tagacaatct
     3781 ggctcatgca ttggccgcgt gtggtgctgg atgcagacgg ttgatcatgt ccgggcggaa
     3841 acctgaccag ctaagatcgc ctgcaatcac gaccggcaac tgacgaaagc cctgagcacg
     3901 cagcgcttcc gccgcttcag gaacgcgatc gacattaatc atttcaaagt caaagccccg
     3961 gttttccatc gcccttttgg tggcgtggca ctgaacgcaa tcgttacgag tgtaaatagt
     4021 aatgcgcatg attcgtattt ccgtttaaaa tgaagatacg gcgcgatgat acgcgtcggg
     4081 ttgtctctct gttgatacag aaatactaga tgtagttgaa aaaagattca accacacaat
     4141 atatagcaaa tagtggtcga aattaccctg ggtatgagcg tgacggggta gggggatttt
     4201 tgtgattcac caggcaaaaa gaaaccccga agccaggctt cggggttaaa gacgcgtatt
     4261 tattatcatg ttgcactacg atttgcgcat gcttaacagt gcgccgataa aaatacctac
     4321 cgcagctgct gtacccacgc tacaccaggg tctttcacga acaaaagaat ccgcgcagcc
     4381 aacggcatcg cgcgcggctt gctggacgcg agtacgacca tgcattcgcg ctctggtttc
     4441 tttcagtaga gcctgcgctt tactacgagc ggcttcggct tcccctttgg cgtcgctgcc
     4501 ccaggacttc aatacagatt cgagactatc agctaattga ttgacatcat tctggatatc
     4561 ctgcacgcca tcatcaacgt cgttgcggtt cggtcggtta aacatatgat cccccattag
     4621 aattcgatgt aattttcagt gtagaccata attttcttaa ctgaggttca tcgcagcttt
     4681 atggactttt ctgaaagcgt tgtgaatgcg caatcaggta aggtaggccc gcggttgata
     4741 ataacgagga tcaaatatgt atttacgacc agacgaggtg gcgcgcgtac ttgaaaaagt
     4801 cggttttact gtcgatgtgg taacccagaa ggcttatggt taccgccgtg gggaaaatta
     4861 tgtctatgtt aatcgcgaag cgcgaatggg acgtaccgcc ctggtgattc atccaacatt
     4921 aaaagaacgt agttcaacac tggctgaacc cgcttccgat attaaaactt gcgatcatta
     4981 tcaacaattt ccgctctatt tagcaggcga gcgacacgag cattacggta tcccgcatgg
     5041 ctttagttcg cgtgttgcgc ttgaacgtta tttgaatggt ttatttg
//
LOCUS       DAZKGF010000254         4961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZKGF010000254 DAZKGF010000000
VERSION     DAZKGF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.254"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(64..822)
                     /gene="yhdZ"
                     /locus_tag="ACHOQV_004741"
     CDS             complement(64..822)
                     /gene="yhdZ"
                     /locus_tag="ACHOQV_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9632992.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /gene="yhdY"
                     /locus_tag="ACHOQV_004742"
     CDS             complement(830..1933)
                     /gene="yhdY"
                     /locus_tag="ACHOQV_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9632993.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRTDCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLPLGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKNQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3124)
                     /gene="yhdX"
                     /locus_tag="ACHOQV_004743"
                     /pseudo
     CDS             complement(1943..3124)
                     /gene="yhdX"
                     /locus_tag="ACHOQV_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3192..4217)
                     /locus_tag="ACHOQV_004744"
     CDS             complement(3192..4217)
                     /locus_tag="ACHOQV_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9632994.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAFNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4648..4869)
                     /locus_tag="ACHOQV_004745"
     CDS             complement(4648..4869)
                     /locus_tag="ACHOQV_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9632995.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT         1375 a         1286 c         1152 g         1148 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg atcacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaactcatcc ggtgcagctt gttccactat ctccccacga tccataaaaa tcaccctgtc
      181 agcgacggtt cgtgcaaacc ccatctcatg tgttacacac agcattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tcaggatcga gcgccgacgt
      301 tggctcatca aataacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc tgcctctttc tttggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtagaac ggtcagatga gggaagagat tgaaatgctg
      541 aaagaccatt cccacttcct gcctgacgcg ctcaatattg cgaatatctt cgttaagctc
      601 gatgccatct accacgatcc gtccctgttg gtgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgcccatac catttattga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaga
      901 agatccagta gatcagtgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caacccgatg atgatcacca
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatc accagcttca
     1081 acgcctgtgg cagaataacc agcccctggt ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccgcc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat caatttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa cagaacggtt atcagcggta
     1321 caccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa cggcagtaag atcccccacg gcagcgcccc cgcaatccca actgatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaagaa
     1501 acccgccata catcagccac cagacaatca gtgggtaaat caccgcccat accgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgacaca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtggatgaag acccaacagg caccggcttt tgtgcagtcg gtacgcgttg
     1741 agccaaccca gttagcctgc aaaaatgccc agttcagcaa cggtggaatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcggggga tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatccg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aatcactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccggat
     2221 tgggtaatcc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagcgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc atcccgccac ggaagttaaa gcctcgtagg gctggaacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacct atgatcaaca
     2461 taacggcgac cggccaggtt cggcgcaatt gtccggtctt tatctggtac gttttattaa
     2521 aacggaatag tccaacagaa atggctatag caataacaac agtcacgata aacgcaataa
     2581 atccgtctcc cagctgcgga gatggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcact cactgcctgg cgtggtccgg gcaaattgcg caacacggca aagtatcaga
     2701 agaagatttg cagcagcggc ggaatattac ggaagatctc aatataaatt gtggaaagct
     2761 ttcgtagcag ccagttatcc gaaagtcgcg ccagaccgat aaagaagccc agaacagaag
     2821 cgaatacaat acacaatgca gaaaccagta gcgtattgag taagccaaca atgaaaacgc
     2881 gtccgtaggt atcaccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc
     2941 cgcgatccag aaaggcaaaa cctgaagtaa tgccacgatt actgagattc gtcacagtgt
     3001 tgtgaaatag ccaaccaaca attccgacaa cagcaacaac ggcaaagatc tggaataacc
     3061 aggcgcgaac cgtagggttg gcaaaggaga gtgagccttt aacggttgag cggcgatgag
     3121 acatagaaac ctcggaaagc ataacgctga agagagcgcc gccgtagcga cgctcgttac
     3181 ctcacaccga cttaacgcac gggcggcgcg tactgaatac cgccgttatt ccagagatta
     3241 ttttgcccac gtttaatttt cagcgggctt tctgaaccta cgttacgctc gaaaatttcc
     3301 gagtagttac ccacctgttt gatgatgtta aaggcccatt tattatccag cttcagatcc
     3361 ttgccgtaat cgccttcttt acccagcaga tgtgccatat caggtgttgc tggattagcc
     3421 gctttttcat cgacgttctg ggaattgatg cccatctctt cagcattcag catggcgaaa
     3481 agcgtccagc gtacaatcga gaaccattca tcatcgccac gacgaactac cggcccaagt
     3541 ggttctttag agataacttc cggtaagaca atccattcag ccgggttgct taatttgatg
     3601 cgcagggcat acagttgtga ttgatccgag gccagcgtat cgcagcgacc agattccagt
     3661 gccttcgctg attcgtcaga gcgatcgaaa gtcactggtg tgtacttcat attgtttgcc
     3721 ttaaagtaat cggcaacgtt gagttcggta tcagtacccg cctgaataca gacggtagcg
     3781 ccatccagtt ctttcgcgct ttttagcccc gctttatcgt gcgtcaggaa gccaatgccg
     3841 tcgtaataag tgacgccagt aaatgccatt cccatcccgg catcgcgaga tgaagtccag
     3901 gtcgtattac gggagagcaa atccacctcc cctgactgta aagcggtgaa gcgttctttt
     3961 gcagtgagcg gggtatattt cactttcgta tcgtcaccga atacagcagc ggcaacacca
     4021 cgacaaacat ctacatcaat acctgaaaac ttaccgtcag catcggcata agagaaccca
     4081 ggtaatccat cactgatccc gcattgcaca aaaccttttt tctgaacggc atcaagcgtc
     4141 gcgccagcat gcgcctgatt tgcaacggca agcagcacgc tggcggcagc cagtgtggct
     4201 atcatcatct ttttcataat gcttcctgtg aggcgaaaat tatcgttata ggacgtttgg
     4261 gaaacgtctt ttacctgtct gtggccatcc tcttataagc aaaagtagtg ccagcttttg
     4321 cgcacgattt cgcgtcgcaa taagctgagt gtagacagga aataataaaa actgcgcccc
     4381 gcaatggtgc gaaaatgaaa catgcgccac attttggcgc aaaatattta cgcgtttttt
     4441 actaagcaga ataatgattg ttagcgtgag tatatttctg gtgggatgtg tgaaagttaa
     4501 attgatgcgt tatttcgaaa gcagtgaaat aacgtgatat aacatgctaa atcagcaggg
     4561 aattacccag cagaatatag caatatttta tcttatggaa agatactgta tcgttaaaaa
     4621 gagcgcggag ctccgcgccc tgtttagtta tttcgacaga gcgatgatcc ccatcgtcag
     4681 gccagcaaca gctgctgccc cgccggcaat agccccagcc gtttcccagt tatcctgagt
     4741 cgtgcctttc atacaggtat tggtgtgaac cagacgaccc tgatcgtcat aaaccggaac
     4801 gcagggcgaa tcgtgcgcac agcccgccag caatgcggtg agcaatgcca caggaattaa
     4861 tcttttcatg gtgttacctc agtaataaac gctaacgctt aataagttaa ctctcttatt
     4921 aacgagctga atgccatttt atcactggag gttataaatc t
//
LOCUS       DAZKGF010000255         4899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZKGF010000255 DAZKGF010000000
VERSION     DAZKGF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.255"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            160..876
                     /gene="nanC"
                     /locus_tag="ACHOQV_004746"
     CDS             160..876
                     /gene="nanC"
                     /locus_tag="ACHOQV_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFP9632996.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            896..2002
                     /gene="nanM"
                     /locus_tag="ACHOQV_004747"
     CDS             896..2002
                     /gene="nanM"
                     /locus_tag="ACHOQV_004747"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP9632997.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLAPVASPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRVYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2067..3047
                     /gene="nanS"
                     /locus_tag="ACHOQV_004748"
     CDS             2067..3047
                     /gene="nanS"
                     /locus_tag="ACHOQV_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9632998.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTCDYSSHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILRFWRER"
     gene            complement(3055..3705)
                     /locus_tag="ACHOQV_004749"
     CDS             complement(3055..3705)
                     /locus_tag="ACHOQV_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9632999.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHQDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HMHHLVPLSAIKEDYQLDPVNDLLPVCPNCHAMLHRRKPPFSPEELKALMDANKSN"
     gene            3842..3985
                     /locus_tag="ACHOQV_004750"
                     /pseudo
     CDS             3842..3985
                     /locus_tag="ACHOQV_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(4693..4776)
                     /locus_tag="ACHOQV_004751"
                     /pseudo
     CDS             complement(4693..4776)
                     /locus_tag="ACHOQV_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025205684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         1431 a         1042 c         1143 g         1283 t
ORIGIN      
        1 aaagagaatt ttcacaggag accttataca aaaaatataa aataagctac ccgttgccaa
       61 agacactata agcctggcaa aaaatattac acaacataaa tgctaattgt ttatgcgggc
      121 tttgtattgc tttctgtatc ctacaaatga gtgaaattta tgaaaaaggc taaaatactt
      181 tctggcgtat tattactgtg cttttcgtca ccattaattt ctcaggctgc gacactggac
      241 gtacgtggtg gatatcgtag cggaagccat gcctatgaga ctcgactcaa agtcagtgag
      301 ggatggcaaa atggatggtg ggcaagcatg gaaagtaata cctggaatac cattcatgat
      361 aataaaaagg aaaatgccgc actcaatgat gttcaggttg aagttaatta cgcgattaaa
      421 cttgatgatc aatggacggt gcgcccggga atgttaacgc attttagcag caacggcaca
      481 cgctacggac cctacgtaaa actgtcctgg gacgcgacaa aagatcttaa ttttggcatt
      541 cgctatcgtt acgactggaa agcttaccga caacaagact tatccggtga tatgtctcgt
      601 gataacgttc atcgttggga tggatatgtc acttaccata ttaatagtga tttcaccttc
      661 gcatggcaaa cgacgctata cagcaaacag aatgattatc gctatgcaaa ccacaagaaa
      721 tgggcgacgg aaaatgcctt tgttctacaa taccatatga cgcccgatat tacgccatac
      781 atagaatatg actaccttga ccgtcagggt gtttacaacg gcagagataa tttatcggaa
      841 aacagttatc gcattggtgt gtcatttaaa ctgtagtaga caggagacag tcacaatgaa
      901 taaaacaata acggcgctta ctatcataat ggcttcattt gccgcaaacg cgtctgtatt
      961 accggaaact cctgtgccat ttaaaagtgg taccggagca attgataacg acactgtcta
     1021 cattggttta ggtagcgcag gtacggcatg gtacaagctg gatacacagg ccaaagataa
     1081 aaaatggaca gcgttagctg cattccctgg cggaccgaga gatcaagcaa cctctgcatt
     1141 tattgatggc aatctgtatg tgtttggcgg cattggcaaa aacagcgagg gcttgactca
     1201 agtatttaat gacgtacaca aatacaaccc caaaaccaat agctgggtta aattgatgtc
     1261 gcacgcgccg atgggcatgg cgggccatgt gacttttgta cacaacggca aggcttatgt
     1321 tactggcggt gttaaccaga atatcttcaa tggctatttt gaagatctca acgaagctgg
     1381 aaaagattca accgctatag ataaaatcaa cgcccactat tttgacaaaa aagcagaaga
     1441 ttatttcttc aataagtttc tgttgtcttt tgatccctca acacagcaat ggagttacgc
     1501 tggcgaatct ccctggtacg gaacggctgg tgcggcggtt gtgaataaag gtgataaaac
     1561 ctggcttatt aatggcgaag ccaaaccagg attgcgaacg gatgccgtat ttgaacttga
     1621 cttcaccggt gataatttaa aatggaataa gcttgctccc gtcgcatcac cagatggcgt
     1681 cgctggcggt tttgcgggga taagcaatga ttctcttata tttgccggag gggccggatt
     1741 caaaggttcg cgggaaaatt accaaaacgg taagaactat gcgcatgaag gcctgaaaaa
     1801 atcatatagc actgatattc atctttggca taacgggaaa tgggataaat ctggtgaatt
     1861 atcgcaaggt cgggtctacg gagtatcatt gccctggaat aatagtttat taattattgg
     1921 cggtgaaact gcaggcggca aagcggtgac ggattcagtt ttaatttctg tgaaggataa
     1981 taaagtcaca gtacaaaact aacgcttcag ggcctcggta aaggggccct gctatcaatt
     2041 tgtcatttgt attacgcgaa aattatatga acgcaataat atcgcccgat tattactatg
     2101 ttcttaccgt tgctggtcag tctaatgcca tggcgtatgg cgaaggactg ccattaccgg
     2161 acagggaaga tgcgcctcat cccagaatta aacaattagc gagatttgcg catacgcatc
     2221 ccggaggccc gtcatgtcac tttaacgaca ttattccact gactcactgc ccacacgatg
     2281 ttcaggatat gcagggttat caccatcctc tggcaacaaa tcatcaaaca cagtacggca
     2341 ccgttggcca ggcactgcat attgcacgga aattactgcc ctttattcct gataacgcag
     2401 gggttctcat cgttccgtgt tgccgtggcg gatcggcttt taccgcgggc agcgaaggga
     2461 catattcaga acggcacgga gccagccatg atgcttgtcg ttggggaacg gatactccgc
     2521 tgtaccagga tttagtcagc agaacgcgag ccgcactggc aaaaaatccg cagaacaaat
     2581 tcctcggcgt atgctggatg caaggcgaat ttgacttaat gacctgtgac tactcgtcac
     2641 accctcaaca ctttaatcat atggttgaag cctttcgtag ggatctaaaa caataccatt
     2701 ctcagcttaa taatattact gacgcaccgt ggttttgcgg cgataccacc tggtactgga
     2761 aagaaaattt ccctcatgcg tatgaagcga tttatggcaa ttatcaaaat aatgttttag
     2821 ccaatattat tttcgtcgac ttccagcaac aaggtgaaag aggactgacg aacgcgcctg
     2881 atgaagatcc ggacgattta agcacgggat attacggttc agcgtaccgg tcaccggaga
     2941 actggacgac ggcactgcga agcagtcatt tcagcgcggc agcccgtcgg gggattattt
     3001 ctgacaggtt tgtagaagca attttgcggt tttggcgcga aaggtgagcg ttcattaatt
     3061 tgatttattt gcatccatca acgcttttaa ctcctccggt gaaaacggtg gtttgcgtcg
     3121 gtgcagcatc gcatggcaat tagggcacac aggcagcaaa tcattaactg gatccagctg
     3181 gtaatcctct ttaatggctg aaagcggcac caggtgatgc atatggataa agccttcagc
     3241 gatatcacca tagatcttca ccagatcaac gccgcatacg ttgcactggc atccatgatg
     3301 ttcgaccgct ttatctcggg cctttttatc gcgttcatag cggtttactg tcacctttat
     3361 cgccgcacct tcaaggtatg tctcctgcga cggcaactca tccgggaaca gaacgtcggc
     3421 tatgggagcc gcagcggctt tatcctgatg aatatgaaat ccctgctcta tgagtagttt
     3481 gacgcattta gattttattc cgccggtgaa atccgcaggg gtaaattgcg tccccgtcat
     3541 caatgtcgct gcgatgccag caatcgcctt cgaggggtaa cgtcggccat gatattcaat
     3601 ctcatacaac cgcgcaggct ggaactgatg agtttctccc gcatcccata aatggatagc
     3661 ttcgatgata taccgccgcg gaagatgatc agggagttta ttcatggaga atgtccttat
     3721 acgttctcag aacaggcagc aaaaaaacaa atgagatcaa ggtaatatat cttcacttgc
     3781 aggcttgaac agcagaaaat ttgcgagcat gatcgcaatt atttcactgc cagggatatc
     3841 aatggcttcc agtaaatcgc tacagcaggc aattgccaat ataaaaatct ggcacaaggg
     3901 tgaacagcgc gcgccgcata agccattgtt attgctatac gtattagcgg gatacctgaa
     3961 tggacatccg cgccttttcg attattgtag tggaacagtc tacgaggatg cagttcgaag
     4021 tgagtaagta aaggttgctg gaaacgatca atgccgccat acaatgattt ttgaggatca
     4081 acaatgagta ataaaaacta cgaaagtcac cgcaaagcga ttgttagtaa aggtatacca
     4141 cccgctctat taaataggct caccaattcg gatgttcagg tgatcaatac cttcttaaca
     4201 cgagtgagca aactggaact atctcaacaa gagaaagact ggatcataaa aatcatctct
     4261 atggtttaga caatttcagc cttgtagcct ttcaagggtt ttgtgtgggt acaaacatta
     4321 tcgaatcggg atatagccgc agctgtagct gtatccacat cagtaagttg atgtaggttg
     4381 acttaggttg agcgaaaaat agcgaaaagc cttgtgtggc gcggatttca gtcataaaaa
     4441 aacgtccgtt gacgtctatt gatgttccga tggtgcgaag gtcgaaggcc ggactcgcct
     4501 ttgcgtttaa ttcgttgaaa aataatggtt tgtgagattt tattttgagg atttgccccc
     4561 aaaatgggac ataaaattga atactgggtg gaagatcaca aagttgcgta attattttcc
     4621 aggtccgaaa cgcattcaag agcaaggcct ctcccggcac tgcgtcagtc gggatgctaa
     4681 atctcccaca tgtagcccta gtaaatcgcg ccagaatgtc aggctagttt gcaaattact
     4741 gacggcgagc gttaagtggt tcagagattg aagcatagga tgcctattga tggttaataa
     4801 tttaaccaaa cactaatagc taataaaacc ccacatggaa agagattttt tgcgggtctt
     4861 gttttgtaat aaatttcttg gcaattactt tatttcata
//
LOCUS       DAZKGF010000256         4863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZKGF010000256 DAZKGF010000000
VERSION     DAZKGF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.256"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            205..1167
                     /gene="tauA"
                     /locus_tag="ACHOQV_004752"
     CDS             205..1167
                     /gene="tauA"
                     /locus_tag="ACHOQV_004752"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9633000.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1180..1947
                     /gene="tauB"
                     /locus_tag="ACHOQV_004753"
     CDS             1180..1947
                     /gene="tauB"
                     /locus_tag="ACHOQV_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFP9633001.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKCIEGPGAERGVVFQNEGLLPWRNVVDNVVFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1944..2771
                     /gene="tauC"
                     /locus_tag="ACHOQV_004754"
     CDS             1944..2771
                     /gene="tauC"
                     /locus_tag="ACHOQV_004754"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFP9633002.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTFSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2768..3619
                     /gene="tauD"
                     /locus_tag="ACHOQV_004755"
     CDS             2768..3619
                     /gene="tauD"
                     /locus_tag="ACHOQV_004755"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFP9633003.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWCWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3659..4633)
                     /gene="hemB"
                     /locus_tag="ACHOQV_004756"
     CDS             complement(3659..4633)
                     /gene="hemB"
                     /locus_tag="ACHOQV_004756"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFP9633004.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1099 a         1232 c         1397 g         1135 t
ORIGIN      
        1 ttataatctg cttgtttatg cattaataaa atgaagtgca gtaacttact gaattgtcag
       61 agtgggtgta attgcatatt taatctttcc tttgccactt tttcgctaag aataaaatca
      121 tctgtgcgat aacaactaat tcttttaatg aatgttttta ttcctgaata ctgctcccat
      181 aacaagacag gggagcagac aatcatggca atttcatcgc gtaacacact tcttgccgca
      241 ctggcattca tcgcttttca ggcacaggcg gtgaacgtca ctgtggcgta tcaaacttca
      301 gccgaaccgg cgaaagtggc tcaggccgac aacacctttg ctaaagagag cggggcgagc
      361 gttgactggc gtaagtttga cagcggagcc agcatcgtgc gggcgctggc ttctggcgac
      421 gtgcaaatcg gtaacctcgg ttccagccca ttagcggtgg cagccagcca acaggtgccg
      481 attgaagtct tcttactggc gtcaaaactg ggtaactccg aagcgctggt ggtgaagaaa
      541 actatcagca aaccggaaga tctgattggc aagcgcatcg ccgtaccgtt tatctccacc
      601 acccactaca gcctgctggc ggcgctgaaa cactggggta ttaaacctgg gcaagtggag
      661 attgtgaacc tgcagccgcc cgcaattatc gccgcctggc agcggggaga tattgatggt
      721 gcttatgtct gggcaccggc ggttaacgcg ctggaaaaag acggcaaggt gctgaccgat
      781 tctgaacagg tcgggcagtg gggcgcgccg acgctggacg tctgggtcgt gcggaaagat
      841 tttgccgaga aacatcctga ggtcgtgaaa gcgttcgcta aaagcgccat cgatgctcag
      901 caaccgtaca ttgctaaccc agacgcgtgg ctgaaacagc cggaaaacat cagcaaactg
      961 gcgcggttaa gcggcgtgcc tgaaggtgac gttccggggc tggtgaaggg gaatacctat
     1021 ctgacgccgc agcagcaaac ggcagaactg accggaccgg tgaacaaagc gatcatcgac
     1081 accgcgcagt ttttgaaaga gcagggcaaa gtccccgccg tggcgaatga ttacagccag
     1141 tacgttacct cgcgcttcgt gcaataaaag gaggcgcaga tgctgcaaat ttctcatctt
     1201 tacgccgatt atggcggcaa accggcactg gaagatatca acctgacgct ggaaagcggc
     1261 gagcttctgg tagttctggg gccgtctggc tgcggcaaaa ccaccttgct gaatctgatt
     1321 gccggttttg tgccttatca gcatggcagc attcaactgg cgggtaagtg cattgaggga
     1381 ccgggagcag agcggggcgt ggtttttcag aatgaagggc tgttgccgtg gcgtaatgtg
     1441 gtagacaacg tggtgttcgg cctgcaactg gcaggtgtag agaaaatgca gcgactggaa
     1501 atcgcacacc agatggtgaa aaaagtgggg ctggaaggcg cagaaaaacg ctacatctgg
     1561 cagctttccg gtggtcaacg tcagcgggtg gggattgccc gcgcgttggc ggcaaatccc
     1621 cagctgttat tactcgacga accgtttggt gcgctggacg ccttcacccg cgaccagatg
     1681 caaaccctgc tgctgaaact ctggcaggag acgggaaagc aggtgctgtt gattacccac
     1741 gatatagaag aagcggtgtt tatggcgact gaactggttc tgctttcacc cggcccaggc
     1801 cgtgtgctgg agcggctgcc gctcaacttt gctcgccgct ttgttgcggg agagtcgagc
     1861 cgcagcatca agtccgatcc acaattcatc gccatgcgcg aatatgtttt aagccgcgta
     1921 tttgagcaac gggaggcgtt ctcatgagtg tgctcattaa tgaaaaactg cattcgcatc
     1981 ggctgaaatg gcgctggccg ctctcgcgtc aggtgacctt cagcattggt acgttagcgg
     2041 ttttgctcac tgtatggtgg gcagtcgcgg cgctgcaact gattagcccg ctgtttttac
     2101 cgccaccgca acaggtactg gcaaaactgc tcaccattgc cgggccgcag ggatttatgg
     2161 acgccacgct gtggcagcat ctggcagcca gcctgacgcg cattttgctg gcgctactcg
     2221 cggcggtgtt gatcggtatt ccggtcggga tcgccatggg gctaagcccg acggtgcgcg
     2281 gcattctgga tccgataatc gagctttatc gtccggtgcc gccgctggct tatttgccgc
     2341 tgatggtgat ctggtttggt attggcgaaa cctcgaagat cttactgatc tatttagcga
     2401 tttttgcgcc ggtggcgatg tcggcgctgg cgggggtgaa aagcgcgcag caggttcgca
     2461 ttcgtgccgc ccagtcgctg ggtgccagcc gtgcgcaggt gctgtggttt gtcattttgc
     2521 ccggcgcgct gccggaaatc ctcaccggat tacgcattgg tctgggtgtg ggctggtcta
     2581 cgctggtggc ggcggagctg attgccgcaa cgcgcggttt aggatttatg gttcagtcag
     2641 cgggtgaatt tctcgcgact gacgtggtgc tggcggggat cgcggtgatt gcgattatcg
     2701 cctttctttt agaactgggt ctgcgcgcgt tacagcgccg cctgacgccc tggcatggag
     2761 aagtacaatg agtgaacgtc tgagcattac cccgctgggg ccgtatattg gcgcacaaat
     2821 tacgggggcc gacttaacgc gcccgttaag cgataatcag tttgaacagc tttaccatgc
     2881 ggtgctgcgc catcaggtgg tgtttctgcg cgatcaagct attacgccgc agcagcaacg
     2941 cgcgctggcc cagcgttttg gcgagctgca tattcaccct gtttaccctc atgctgaagg
     3001 ggttgacgag atcatcgtcc tggataccca taacgataat ccgccggata acgacaactg
     3061 gcacaccgat gtgacattta ttcaaacgcc acccgcaggg gcgattctgg cggcgaaaga
     3121 gttaccctcg accggcggcg atacgctctg ggccagtggt attgccgcat atgaggcgct
     3181 ctctgttccc ttccgccagc tgttgagcgg gttgcgtgcg gagcatgatt tccgtaaatc
     3241 gttcccggaa tacaagtacc gcaaaaccga ggctgagcat caacgctggc gtgaggcggt
     3301 cgcgaaaaat ccaccgttgc tacatccggt ggtgcgaacg catccggtga ccggtaaaca
     3361 ggcgctgttt gtgaatgaag gctttaccac gcgaattgtt gatgtcagcg agaaagagag
     3421 cgaagccttg ctgggctttt tatttgccca catcaccaaa ccggagtttc aggtgcgctg
     3481 gtgctggcaa ccaaatgata ttgcgatttg ggataaccgc gtaacccagc actatgccaa
     3541 tgcggattac ctgccacagc ggcggataat gcatcgggcg acgattcttg gggataaacc
     3601 gttttatcgg gcggggtaag ggggaaatgc cgggttggat aacccggcat aatgatcatt
     3661 aacgcagaat cttcttctca gccaaatcca gcgcaaagta gctgaaaatc agatccgcac
     3721 ccgcacgctt aatcgaacct aagctttcga gcacgacttt ctcttcatct atagcacccg
     3781 ccagcgcggc gaacttaatc atcgcgtact caccgctcac ctgatacgcg ccaatcggca
     3841 attcagtacg ttcacgcagc tcacgcacga tgtcgaggta tgctccggca ggtttaacca
     3901 tcaggcagtc tgcgccctgg gcttcatcca gcagtgactc acgaatcgcc tcacgacggt
     3961 tcattgggtt catctgatag cttttgcggt cgccttttaa tgcgcttccg gcagcttcac
     4021 ggaacgggcc ataaaaggag gaggcgaact tggtcgaata cgacataatc gccgtatctt
     4081 taaagcccgc agcgtccagc gcctggcgaa tcgcctgtac ctgaccatcc atcgcagcgg
     4141 aaggggcgat gaaatctgcg cctgcagcag ctgcaaccac ggcttgcttg cctaaatttt
     4201 ccagagtcgc gtcgttgtcg acgccatgct cgcacagcac accgcagtga ccgtgagaag
     4261 tgtattcaca gaaacaagtg tctgacatga cgatcatttc tggcacagtc tgcttgcaga
     4321 tgcgcgacat acgcgccacc agtccatctt cccgccaggc gtcgctgccg gtttcatcgg
     4381 tatggtgaga gatgccgaaa gtcatcacgg aacgaatacc ggcgttggcg atgcgttcaa
     4441 tttcgcgtgc cagatgtttc tctggaatgc gcatcacgcc tggcatggct tcaacggctt
     4501 tgtagtcgtc aatttcttct tcaacaaaga tcggcaacac caggtcgtta aggctaagtg
     4561 ttgtctcttc aaacatagcg cgcagcgcag gagatttgcg caggcgacga gggcgttgga
     4621 ttaaatctgt catggtctgc ctgatgtttg tggaatcgag gagcatagta tacctgaagc
     4681 aggctgggtg tgttttacga aagttgtcgc gatgttgaca agaagagaat gaaaaagagg
     4741 tcaggacatc tttgtaatag aaatagcaat aatttcatat tcactgaaaa tatttttaat
     4801 ttttatttat agccatagat tattgttttt ggggatttgg tgacgttttc aaaaagtgaa
     4861 taa
//
LOCUS       DAZKGF010000257         4826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZKGF010000257 DAZKGF010000000
VERSION     DAZKGF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.257"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(277..1479)
                     /locus_tag="ACHOQV_004757"
     CDS             complement(277..1479)
                     /locus_tag="ACHOQV_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP9633005.1"
                     /translation="MKINRHGITLSLISFLIAGAPAFAQDGSSWASPDTASASETDST
                     AVGAWANAGKAGTALGSASYAHAPFSTSVGMSAYVNPEASNGVAVGSWASADALNSVA
                     LGTGAQAMHRYSVALGQQSGTTRENEVYIGYDSGVTNPASPRVPDKTRVLGGVSDGTR
                     DTDAATVGQLNRKADEVYSDVSGRIAAEALKARDHTDTVAAENRENIIRNTVAINRNT
                     RGLLSQRDVLETHEERLNSQQQQINTGSTVAVDSHGYVTRGEGTGERITVQEGLVRTQ
                     EMATENRAAVSRNRQVGERNSRAIASQARTLQAHERRMNSQQRQINENHEEMKRAAAQ
                     SAALAGLFQPYSVGKFNATAAVGGYSDKQAVAVGMGYRFNEQAAAKAGTAFSDGDVSW
                     NVGVNWEF"
     gene            complement(1747..3615)
                     /locus_tag="ACHOQV_004758"
     CDS             complement(1747..3615)
                     /locus_tag="ACHOQV_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633006.1"
                     /translation="MGVFNYKSYSFAHVAELVSSSYNLAIYSNTGKVLGLGSFLASRL
                     FAKHPDRINYSTDVLNEGWRDITVEELRLSSEYVDSRGFYRFPSPITGNPASGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAVSNG
                     LSGEDVIVTGYSLGGGLTNIMARLSESLADGFYANSDYIAWASPLVFDGDGRVLNIGF
                     ENDPVFRVLGDESGFWEAVKAMGPGLTTPDREYDSSPDNIVLYTDLYGSFLWNIIPFS
                     VLALPLGSWSAHFAGSKTDAFERILGSQFYDYMSPDSTVIVDEQSGWTSWYSWVHDKG
                     DDSRERPVFIIGNDGNNLLAGGQGGDYIEGAGGNDKIEPGKGANRVDGGSGYDTLLLG
                     DVMDDWNIYRVSDGTLFFHSERGAGLTEATGIEGISFSGDWRSTLSPYQIGEEQITDH
                     RYLLKWRNKNVDYGKHTEGTTGDDSLYGDIIFGLDGNDILRSAGGEGGSLLHGGCGND
                     YLHGTSGNDELYGAEGNDWLYGGGGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHKG
                     EEDFLFLSKDIVTSTDEFISCLKQTGNDVEAGIAGGRIYFVNTALEDINHNSVYIV"
     gene            complement(3775..>4826)
                     /locus_tag="ACHOQV_004759"
     CDS             complement(3775..>4826)
                     /locus_tag="ACHOQV_004759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633007.1"
                     /translation="DRIGTVIYTGNVNMTQGHGITAGSGQKVKISDDDLQKDSKSFLA
                     IKAHEANVGTNVYVISKNGANNVALLEMTEGDNIITTELSLGNLGVSRWVRPMVVDTG
                     AGDDTIVTGRDLGIGYGDLWTEVNTGIGDDILIVGFGRGGYDVVYGKDGSYRIEETAK
                     YQRLDGDIIIPDNDSGYPNNGGFIEKTTVTMGDGNDSLLLLGGNSLAALKSSTLDLGA
                     GNDVIDIVANIDNASNIKGGSGFDTLVIHQGNATSNTFSGFEKIELKDGAALNLLAEN
                     LNSASIEGNKLKVTGDGSSRVDLGNNGDNLSDNGYNWIKGSTESEGDITYNIYTHASV
                     PDVQVLIDERITNVV"
BASE COUNT         1391 a         1332 c          848 g         1255 t
ORIGIN      
        1 agactcagct ctttcagttc cccttctcct tcagcaagaa gacaacattc aaaaacagct
       61 gcatcgtcgc attccagacg aacctgcacc ctgtcagccc gaccgtttac agtaaatacc
      121 tgcactgaac ttatgcgata gttcagcata tacactctcc ctctcagctc ctcaggaata
      181 ccccggaacc gtcagtcatg ccaccgggga gaaaagcagg cagcgaatca tgaccgcata
      241 cagaacgctg cccgccccgg tgactcagac ccgggttcag aactcccagt tgacacccac
      301 attccaggaa acatcaccgt cactgaacgc agtacctgcc tttgccgcag cctgttcgtt
      361 aaagcgatat cccatgccca ccgcaacagc ctgcttgtca ctgtacccgc ccacagccgc
      421 tgtggcgtta aatttaccca cactgtatgg ctggaaaaga ccggccagcg ctgctgactg
      481 ggctgctgcc cgcttcattt cctcatgatt ctcattaatc tgacgctgct gactgttcat
      541 acgacgctca tgcgcctgca gggtacgtgc ctgagaagca atggcgcgac tgttgcgttc
      601 acccacctga cggttacggc ttaccgcagc acggttttct gtggccattt cctgtgtacg
      661 caccagccct tcctgtacag taattcgttc accggttccc tcaccgcgcg tcacataacc
      721 atgactgtcc acagcaacgg tacttccggt atttatctgc tgttgctggc tgttcaggcg
      781 ctcttcatgc gtctccagca cgtctctctg ggaaagcagc cccctggtgt tacggtttat
      841 ggccaccgtg ttccggataa tattctccct gttctctgca gcaaccgtat cggtatggtc
      901 acgcgcctta agtgcttccg ctgcaatccg tccactgacg tcactgtaca cctcatccgc
      961 cttcctgttc agttgcccga cagtggctgc atccgtatcc cgtgtaccat cactgacacc
     1021 accgagaacc cgggttttat ccggtacacg cggactggcc gggtttgtga cacctgagtc
     1081 atatccgatg tagacttcat tttcacgggt tgtaccggac tgctgaccaa gagccactga
     1141 atacctgtgc attgcctgtg cacccgtacc cagtgctact gaatttaaag catctgcaga
     1201 tgcccagcta cctactgcca caccattaga cgcctccgga tttacataag cagacatccc
     1261 cacggatgtg gaaaacggcg catgcgcata cgatgcagag ccaagagctg tccctgcctt
     1321 cccggcattc gcccatgccc ccactgccgt ggagtcagtc tctgatgcag aggctgtgtc
     1381 cggagacgcc caggaactgc catcctgcgc aaacgccgga gcaccggcaa tcagaaagga
     1441 aataagcgat aaagtaatac catgtcgatt tattttcata aacaacctca ttgatataca
     1501 caatatactg gcacagagtt aaattaatat ataatcagga gagaagtgaa agagtacccg
     1561 gtgcgaaaat gaattaatct gagcagattt aacggtaaac gaagcgcaat aaattaaaat
     1621 taaaaaaaca aaatcacttg cagtctatta tctttacgtt cctcaccgca accgcacaca
     1681 aaaacaaaac acacaatgca ttgattttaa acaaaaaaaa caaaacagaa actcaacaca
     1741 taaaaatcag acaatataaa cagaattgtg atttatatcc tcaagcgctg tattaacgaa
     1801 atatatccgt cctcctgcaa tacctgcctc aacatcattt ccggtttgtt taagacatga
     1861 aatgaactca tctgtcgatg taacaatgtc ctttgataaa aacagaaaat cttcttcacc
     1921 cttatgttta ttaaaatcca tgataaatga attcccttca caagcagcat caaagacgaa
     1981 gacatcatga ccagtaccac catacagata atcattacct cccccaccat ataaccagtc
     2041 gttaccttct gccccgtaca attcatcatt accagaagtc ccatgaagat aatcattccc
     2101 acaccctccg tgtaaaagac ttcccccttc tcctccggca ctacgtaata tatcatttcc
     2161 gtcaaggcca aaaattatat ctccgtacag actatcatct ccggttgtac cttcagtatg
     2221 tttgccataa tcaacatttt tattacgcca cttcagaaga tacctgtggt ctgtaatctg
     2281 ctcttctcct atctgataag gacttagtgt actcctccag tcaccagaaa aagagatacc
     2341 ttcaatacct gttgcctcag taagaccggc tccccgttct gagtggaaaa acagtgtccc
     2401 gtcactgacc ctgtatatat tccagtcatc cattacatcg ccaagaagaa gcgtgtcata
     2461 cccactgcca ccatcaaccc tgtttgctcc cttacctggt tcaatcttat cattccctcc
     2521 tgccccctct atatagtcac caccctgccc acctgccagc agattattgc catcattacc
     2581 gatgatgaat acaggtcttt cacgactgtc atcccccttg tcatgaaccc atgaatacca
     2641 ggatgtccag ccactctgct catccacaat gactgtactg tccggtgaca tgtagtcgta
     2701 aaactgagac cctaaaattc tttcaaaggc gtcagttttt gaacccgcaa aatgcgcact
     2761 ccagcttccc agcgggagag caagcacaga aaaggggata atattccaga ggaatgaacc
     2821 ataaaggtct gtgtataaaa caatgttatc aggagaagag tcatattcac gatccggggt
     2881 tgtcaagccc ggccccattg ctttaactgc ctcccagaac ccggattcat ctcccagcac
     2941 acgaaatacc ggatcgtttt caaaaccgat atttaatacc ctcccatccc cgtcaaatac
     3001 cagcggggaa gcccaggcta tatagtcaga attagcataa aaaccatcag ccagagactc
     3061 actgagtctg gccataatgt tagtcaaccc gccccccagg ctatagcctg tgacaataac
     3121 atcctcacca cttaaaccat tactgactgc aaagtctctg atactttgaa gcaggttttc
     3181 tgcttcagac agcgcttttc cattattgag ctgtgtataa tcaacaatat cgcagatatc
     3241 atttgttcca cagaatgata ttgcaatttt ctgtagattc tgttgagcat caaacaaccc
     3301 aacaactttc aactgaggac caaatcctga cgcagggttt cctgtaatcg gactgggaaa
     3361 gcgataaaaa cctcttgaat cgacatattc cgaagataac ctgagttctt ccactgtaat
     3421 atcacgccag ccttcattaa gaacatcggt agaataatta atcctatccg ggtgctttgc
     3481 aaatagcctg gatgcaagaa aacttcctaa tccaagaacc tttccagtgt tggaatatat
     3541 agcaagatta taagaagaag aaacaagttc tgctacatgt gcaaaagaat aagatttata
     3601 attaaacacc cccataaaat ctcctctgaa ttcatttaaa tatccttcat ttatccaaga
     3661 agaaacaaaa agcattattc tcagataaat tttttcagta aaagagcata cctggagtag
     3721 aacttacaac gaaaaagatc cccccacagc aacgactgtg aggggatact attgttacac
     3781 tacattagtg attctctcat caatcagaac ctgtacatca ggaaccgaag catgtgtata
     3841 aatgttataa gtgatatccc cttcagactc agtgcttcct ttaatccagt tatacccatt
     3901 gtcactgagg ttatccccgt tattaccgag gtcaacccga gaactaccat cacctgtgac
     3961 tttgagttta ttaccctcta tagaggcaga atttaaattc tcagccaaca aattcagtgc
     4021 agctccatct ttcagttcaa tcttctcaaa accagagaat gtgttgcttg tggcattacc
     4081 ctgatgaata accaaggtat caaatccaga accaccttta atatttgaag cattatcaat
     4141 attagccaca atatcgatta catcattacc agcacctaaa tccaaggttg atgatttgag
     4201 cgcagcaaga gaattacctc ctaacaataa caggctgtca tttccatctc ccatagtaac
     4261 agtcgttttc tctataaaac caccattatt tgggtatccc gaatcattat caggtattat
     4321 gatatctcca tccagtcgtt gatacttagc ggtttcttca atgcggtaag aaccatcctt
     4381 cccatatacg acatcataac cgcctcgacc aaaaccaact atcaggatat catcaccgat
     4441 acctgtattt acctcggtcc atagatcacc atatccaatc cccaaatcac gaccagtcac
     4501 gatggtatcg tcacctgcac cagtatcgac aaccattggt cttacccagc gagaaacgcc
     4561 cagattcccc agtgacagtt cagtcgtaat tatattatcc ccctctgtca tttccagtag
     4621 agcgacgtta ttagcaccat tcttacttat tacataaaca tttgtcccta catttgcctc
     4681 atgagcctta atggccagga atgatttaga atctttttgt aaatcatcat cagagatttt
     4741 gaccttttga cctgagccag ctgtaattcc atgaccttgc gtcatgttta cattgcctgt
     4801 atagataacg gttccaatcc gatcta
//
LOCUS       DAZKGF010000258         4813 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZKGF010000258 DAZKGF010000000
VERSION     DAZKGF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.258"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..301
                     /locus_tag="ACHOQV_004760"
     CDS             <1..301
                     /locus_tag="ACHOQV_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9633008.1"
                     /translation="TLRDSAKHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNM
                     TFSPSRNGTSLDLQAGLEARIRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            412..2796
                     /locus_tag="ACHOQV_004761"
     CDS             412..2796
                     /locus_tag="ACHOQV_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFP9633009.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRKDSFIGKICGWIGTKEWGW
                     ENYNVDVSRSVININKVRKEVMSLTRAYFGELQASIEQNINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTERLQALNERVPELITDSKALREELETLL"
     gene            2793..3698
                     /locus_tag="ACHOQV_004762"
     CDS             2793..3698
                     /locus_tag="ACHOQV_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP9633010.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTTG
                     ERTFFRRLKEGMTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            3695..4765
                     /locus_tag="ACHOQV_004763"
     CDS             3695..4765
                     /locus_tag="ACHOQV_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9633011.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
BASE COUNT         1246 a         1161 c         1380 g         1026 t
ORIGIN      
        1 caccctgcgc gacagtgcaa aacacagtgt gagtgaactg ccggtgaact ggtgggtaca
       61 gccttctgtt atccgcacct tcagctcccg gggtgacatg agcatgggga cagccgcagc
      121 cggcagtaac atgacgttct caccgtcccg gaatggcacg tcactggacc tgcaggccgg
      181 actggaagcc cgtatccggg aaaatatcac cctgggcgtt caggccggtt atgcccacag
      241 cgtcagcggc agcagcgctg aaggctataa cggtcaggct acgctgaata tgactttctg
      301 attatacggt atcccggaaa tacgatttca ggaaaattca tgcagtaatg ggaaaggacg
      361 ctcattgctg ttcaccgcag ttctgaatta ttgaatttat aaaggtgaat gatgcacgaa
      421 aagaacatcg ccctgctttg tgatgaagcc gaccggcttt tgcaactgaa tatcaatctg
      481 ctccggcaaa tggttgatga gccggatgtg ttatctgaca gtaagaacga aaacgggctg
      541 ctttttgata aacggaaagc gctgaaaaga attgaggagc tggagggcga acaaatcaaa
      601 accgcccgca gggagatggt gctggctgtg gtcggcacga tgaaggcggg caaatcaacc
      661 accatcaacg ccattgtggg gcaggaaatt ctgcccaacc gtaaccgccc catgacctcg
      721 gtaccgacat tgatccgcca cgttcccgga aaaacggaac cggtcctcca tctggaacat
      781 attcagcccg tccgcaattt attaatcaca ctgcaggaaa aactcgccac cccggcaggg
      841 cagcaagtcg cacagtccct gcagcaaacc ggggataccc gcgaactact ggatattctg
      901 gcggatgatg tctggcttaa aaatgaatac cacggggaag acgaaatctt taccggactg
      961 gcatcgttaa atgacctggt tcgtcttgct gcggcaatgg ggactgaatt tccttttgac
     1021 gaatacgcag aagtgcagaa actaccggtg atcgacgtgg cgttcagcca cctggtggga
     1081 atggatgcat gtcagggaac actcacactg ctggataccc ccggccctaa tgaagccgga
     1141 caaccacaga tggaaatgat gatgcgggat caactacaga aagcctctgc ggtgctggcg
     1201 gtgatggatt acacccagat gaactcgaaa gccgatgagg aggttcgtaa agagcttaat
     1261 gccattgctg acgtctcagc cggtcgtctg tttgtgctgg tcaataaatt cgatgaaaaa
     1321 gatcgcaatg gtgatggtgc ggatgccgta cgccagaagg tgccagcaat gctgaacagc
     1381 gatgtgctgc ccgcctcccg cgtttatccc ggctcatcac gtcaggcgta cctggctaac
     1441 cgtgcgcttc atgagttacg aaaaaacggt gcgcttccgg ttgatgaagc ctgggtcgat
     1501 gattttatca gggaagcttt cggacctatg gtggaagagg acgactggaa agacagcact
     1561 aaggtaaata aaaaggcaga aaaactctgg aatatatctc tgatcgatca actgataacg
     1621 gaagtcatac agagctccca ttccagagcg gcggcactgg cggttgactc tgccgccgca
     1681 aaactgatgc agaatgcaga aaatgtcagc gaatatctgt cattacgtca tcaggggctg
     1741 cagcaaagca ttcagtcact acaggcgcat attaccagcc tgctggcgga tatccaggag
     1801 attgaggaat gtcagaatca ggtgaccggt gatgtcagaa tggccatgga ggacatcaat
     1861 accaaaaccg gggaattact gacgaaggtc tgtgcctcac tggaggagga gctgaacgac
     1921 tatttcagaa gcggtaaacg caaagaacag caaatgctgg aggaagaaaa ttcagcgcag
     1981 ccccgggaga gaaacgcatt cgctttcttt cacgatattt tcggaaccgg aaatcagcat
     2041 gacaggatgc gggactttga tccggacagc cccgaaataa aattcagtga tcgcagggca
     2101 gcccttgaac tgatgacgca aatcgaatcg accgtgacca gcctgcaccg tgaggctgaa
     2161 gcacagttcc ggcctgagct ggagaaaatc gtcagcggga ttgaaacagg ttttcgtggc
     2221 acggccctgt acgccacaga aaacattgcc ggtcgcatca atgcccgcct ggaggatgag
     2281 ggctttaccg taaaaatcag ttttccggca gtcagccagt tacagacccg gctcgcggta
     2341 aaaataaatc tgagtgcgct tatggaggaa agaacggaga cagtcacccg tcgccgtcgg
     2401 aaggatagct tcattggaaa gatttgtgga tggattggca ccaaagaatg gggatgggaa
     2461 aactataacg tggatgtgag ccgcagtgtt atcaatatca acaaggtcag gaaggaagtg
     2521 atgtcactga cccgggcata tttcggggag ctgcaggcat ccattgagca gaatattaac
     2581 cagcccgttc gccaggaaat cgatgccttt ttctgcgcat tcagggaaaa agttgaacaa
     2641 ctgcgtaaca cgctgattca gagttctgaa gatcataaac gcgatcagca ggcgcaggaa
     2701 cggcttacgg agcgccttca ggcattaaat gaaagggttc ccgagttgat tactgacagt
     2761 aaggcgttaa gggaagaact ggagacactg ctgtgacctc accatttatt caacaaattg
     2821 ctgataaccg ggtatgtcag gtactcacct gtcttcctga aaaatttgta gttgattttg
     2881 caaacggtat cgatgttgca caggagcata tccgcacgac cggagagcgc acgttcttcc
     2941 ggcgtttaaa agaggggatg accggcgaag gcgctgcacg gcagaatgcc atcaatgcgt
     3001 cgcttgcaca gggcgttgag gcgtccctgc gctggctgac ggagatgaca acgtcacttg
     3061 ccaccacaaa ctacgcgatt acccgggtaa acgacagggt cagttcactg gtcagtgata
     3121 cagccaggct ggcgcattat tccgcagata cgcgggagca gttactcacc ctggccgatc
     3181 aggttcacca taaactgaat catcttgaag aaaaactcca ccgtgttgac caggttcagc
     3241 gggcgcagtt acatcttgag cagatattct catggtggag cgccgggcga tatgcgtcat
     3301 tttcccctgc cggacgttgt tatgtggcac ttgaagagct tcgctggggc gcgtttggtg
     3361 atgtgatacg tcagggcgaa acaggccagg ttaaccagct actggatatt ctcagacata
     3421 aagcattaac gcagatggca caggagagtg gcggtagtgc aaccgtgcgt ctgaacactc
     3481 tggactggct tggtggtcag ggccgggagc aggcagataa cgagtggcat gacgcgatta
     3541 actggctggg agactggtgc agtgaagagc agcatccggt gatctggtca accacgcagg
     3601 ctgcagaaca cttaccggtt cgcatgcctc gcctctgctc tgcagaacgc ctctctgaaa
     3661 gtatggtcga tgaaatattt cagaaggggg ctgcatgagt acagaaatga aaacggggct
     3721 ggtgctgtcc ggtggcgggg cggtgggcgc ttatcaggcg ggagtggtta aggcactggc
     3781 agagtgtggt acacagatca gcatggtttc aggggccagc attggcgcat tcaatggtgc
     3841 cattatcgcg gcctctcccg atctgtcaga agctgccgta cgcctggagg cgctctggga
     3901 tcatctgggg aataatcagg tgctgtcggt aaacagattg gtttactttt cattgctgaa
     3961 aaaattgttc caggcaatga acctctgcca gatccccgga cgtgcaggag cactgcttac
     4021 gacgcttctt cgccatatat cgacaatcaa cgggtttgac aatctgatgg ctcagccgtt
     4081 gttgtcagat gagcccctga cagcgctgat ggatcattat cttgatactg atgctctggc
     4141 agacgggcta ccgctgtatg tgtcgctgta ccccacagaa gggggcatgc aggatattat
     4201 tgactgcatt cgtgctgaac tgggtgtcgg aaccacgaaa aacgccgttt ttcagcatat
     4261 ccagagcctg ccccgcggac agcagaaaga ggctctgctt gcgtcagccg cgctgcccct
     4321 gctgttccgt ccccgtgagg ttcaggggac aatgttcggt gatggtggta tgggaggatg
     4381 gcgaaatatg cagggaaata cccctgtgac gcctctggtc gatgccggat gcaatatggt
     4441 gattgtgacg catctgagtg acggttcttt atgggatcgc caggcttttc cggacaccac
     4501 aatccttgag atccgtcccc ggaaaaggtt gaaatatgca ggtgatggtg gcaacagcgg
     4561 cggtctgctc agttttacat cggcacatac cgacgcctgg cgtcagcagg gctatgaaga
     4621 cacgatgctg gcgatggagc atatccggaa accgctggca gcacgtcagg cactgacccg
     4681 gtcagaggcg gtattgcaga aaagcctgga tataacggaa gaggcagatt tggcactgag
     4741 aaacgcgatg gcccggatta aataaagacg ccccaaagaa acgccaccgc ctgtggtggt
     4801 gtttttcctg tct
//
LOCUS       DAZKGF010000259         4684 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZKGF010000259 DAZKGF010000000
VERSION     DAZKGF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.259"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>4684
                     /locus_tag="ACHOQV_004764"
     CDS             <1..>4684
                     /locus_tag="ACHOQV_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFP9633012.1"
                     /translation="QDGVLSNRNTKTRGGISSAGTLTVRAGMLNNQQEFIAGQKDVTL
                     NTGTLDNRQGALGSQASLQVSSGTLMNQKGALKAGTDMLLSGDDVSNQEGTLAAGGDL
                     NAHLNVLENQQGTVVSNGNSRLDVTRSDNQGGRLVAQQSLTLTSKDIINDAGGLIQSG
                     ASLNIRADTLSNRDSGDRGGVISQGPMTLNAGTLDSTAGVLLSGDALSLTAGVVNNTS
                     GQIVASGQIVANGLPGRNSLALNNQSGLIQGKGISINTAGRTLDNRRGTLNSLQELTV
                     STGAMDNRGGTVGAKTTADLSTTSLDNREGGRLVSEGELRLHTGGLQNSHGQIQSVGN
                     MLLDSVRGVVDNVSGLIRSGSAITLNALQFINRHTQNTGQGLEAQTIHITTQDLDNQE
                     GSILADRALTVMADRTLSNNDGVLSSGATLSVSGRQLAFSNRDGVVKAGQSVSVTAGQ
                     LGGDGKLLSPGDITLKSNTAFSNSGQTIANGNLTLSVNGDVTNTGSLLAGSRLDLNSI
                     RLENTEKGEISAGQTWLNVTDTLLNRGLIDGKYTRLQANTLTNSGTGRIYGDAVGVSA
                     ATFNNLEENGVAATLAGRERVDLGVQTLNNRTHSLIYSAGDMYIGGTLDANGSATGKA
                     GVLNNHSATVEATGSLALSAGQINNVNDHFTTEHVVVSTEKVTEYQLSGSDKRWRAGE
                     PDVYVDNDSSNSLKKLHTPEGARDKFTQYDYTRTVEETRVKDSDPGKILSGAGMTIVA
                     DKLLNDKSQVVAGGLLTIPSGSVENVSVSGERHVTDSGTSTYYYRIRKKGKDKQGEKT
                     SQYTPPTVIQTITLKPGELTSHGQVQGSQVTLSPLKPQGTDVQTGLTGNVDAAVAATD
                     RIPLRPVVSAGEPVILLPGQQFEVSTPQGSIHVAGPDTRLPDSSLFKTNPAVNVPYLV
                     ETDPRFTNQKMWLGSDYMQKAFSQNGDNMLKRLGDGFYEQRLIREQVVSLTGQRYLDG
                     YGSDEEQFKALMDAGIVFGKQYNLKPGVALTAEQMALLTGDIVWLVNTTVTLPDGSTQ
                     TVQVPQVYARVKPGDVNSAGALIAGRDMVMKLDGDLFNSGKLAGKQTVQLSAENIHNQ
                     AGTIQGANVSLTARTDINSTGGLLQATDSLLAMAGRDISLTTTTRTAQRDAGQNHFER
                     TSIDSVAGVYVQNDQGRLVLQAGRDMNLTAATVVNQGKNSLTQLSAGRDMTLSTVTTS
                     AQDNIIWDKNNRLSQGVTQSTGSTLAGNGDVTLTAGRDMTSQAASLSAQKGLALMAGH
                     DVTLTGAQNTSSLDEYHKVTGSSGMLSKTTTTTHDVTDRRTMTGSELNGDTVSISAGH
                     NLNVTGSSVAGDNRVSLAAGNDLSIGTLTESNRETHLKQEKKSGLMSSGGVGFSVGSQ
                     SLKVTDTATDSTQKGSTVGSVHGDVSLQAGNRLSANSADLIAGRDMALSGKEVSISAV
                     TDRHVQTHTVEQKTSGLTLALSGTVGSALNTTVETVQAAKSAGNSRLEALQGVKAALS
                     GAQAVQAGRLAEAQGNQKENNNAVGISLSYGSQSSKSEQRAEQTVAKSSALTAGNNLS
                     IQATGSGVK"
BASE COUNT         1175 a         1190 c         1464 g          855 t
ORIGIN      
        1 caggacgggg tactcagtaa ccggaacacg aaaacacggg gtggtatcag cagcgccggt
       61 accctgacag tacgggccgg tatgctgaat aaccagcagg aatttattgc gggtcagaag
      121 gatgtgaccc tgaacaccgg aacgctcgat aaccgtcagg gcgcgctggg gagccaggcg
      181 tcactacagg tatcgtcagg tacgctgatg aaccagaaag gcgcactgaa ggccgggacg
      241 gatatgttgc tgagtggtga cgatgtcagt aatcaggaag ggacgcttgc cgccggcgga
      301 gacctgaatg cgcacctgaa tgtgctggaa aaccagcagg ggacggtggt cagcaacggt
      361 aacagcagac tggatgtcac ccgctctgat aatcagggtg gcaggcttgt ggcacagcag
      421 tcactgacac ttacctcgaa agatatcatc aacgatgccg gcggcctgat acagagcggt
      481 gcatcgctga atatccgtgc tgacacactg agcaaccgtg acagtggtga ccggggcggg
      541 gttatcagtc agggcccgat gacactgaat gccggcacac tggacagcac agccggagtg
      601 cttttgtcgg gtgatgcgct gtccctgact gcaggcgtgg ttaacaatac atccggtcag
      661 attgtggcga gtggtcagat tgtggcgaac ggtctgcctg gccggaacag tctggcgctg
      721 aacaatcagt ccggtcttat tcaggggaaa ggtatcagca ttaataccgc aggccggacg
      781 ctggataaca gacgcggtac gctgaacagc ctgcaggaac tgaccgtcag taccggagca
      841 atggataacc ggggcgggac tgtgggggcg aaaacgacag ctgacctgag caccacctcg
      901 ctggataacc gtgagggtgg tcggctggtc agtgaaggtg aactccgtct gcatacggga
      961 ggtctgcaga acagccacgg acagatacag tctgtgggca acatgctgct cgacagtgta
     1021 cggggtgttg tggacaacgt ctctggtctg attcgcagtg gttccgccat tacgctgaat
     1081 gcactgcagt ttatcaatcg ccacacgcag aataccggcc aggggctgga agcgcagaca
     1141 atacacatca cgacgcagga ccttgataac caggagggca gtattctggc agacagggcg
     1201 cttacggtta tggcagacag gacactcagt aataatgacg gcgtgctctc ttccggcgcg
     1261 acattgtctg tcagtggccg tcagctggcg ttttcgaacc gtgacggcgt ggttaaggcc
     1321 gggcagtcag tatccgtgac agccgggcag cttggtggtg acgggaaact gctttcgccg
     1381 ggcgatataa cgctgaaaag taacacagcg ttcagcaaca gtggtcagac tattgcgaac
     1441 ggaaacctta cgctttccgt taatggcgat gtcactaata ccggcagcct gctggcgggc
     1501 agccggcttg acctgaacag catccgcctt gaaaacacag agaaaggtga aatcagtgcc
     1561 gggcagacct ggcttaacgt gacggacaca ctgctgaacc gtgggctgat tgacgggaaa
     1621 tatacgcgcc ttcaggcgaa caccctgacg aattccggta ccgggcgaat ctacggcgat
     1681 gctgttggtg tcagtgctgc cacatttaat aatctggaag aaaatggcgt ggcggcgacg
     1741 ctggccgggc gtgagcgtgt ggacttaggg gtacagaccc tgaataaccg gacgcacagc
     1801 ctgatttaca gtgcaggcga tatgtatatc ggtggcacgc tggatgcgaa tggcagcgcc
     1861 acaggtaaag ccggtgtact gaataatcac agtgccactg ttgaagccac cggttccctg
     1921 gcactgagcg ccggacaaat caataacgtg aatgaccatt tcaccacgga gcacgtggtg
     1981 gtttccacgg aaaaggtgac tgagtatcag ctcagcggct cagataaacg ctggcgcgcc
     2041 ggtgaaccag acgtttatgt ggataacgat tccagtaact cactgaaaaa actgcatact
     2101 ccggaaggtg ccagagataa attcacgcag tacgattaca cccgtaccgt ggaagaaacc
     2161 cgggtaaaag actccgaccc gggaaaaatt ctgtccggtg ccggcatgac gattgtggca
     2221 gacaaactgc ttaatgataa gagtcaggtt gttgcaggcg gcctgctgac cattccgtca
     2281 ggaagcgtgg aaaatgtcag tgtcagtggc gagcgccatg ttacggactc agggacatcc
     2341 acgtattatt accgtatcag gaaaaaagga aaggataagc agggggagaa aacctctcag
     2401 tacactccgc ccactgtaat tcagaccatc acgctgaagc cgggagagct caccagccac
     2461 ggacaggttc agggcagcca ggtcacgctt tctcccctga agccacaggg aacggatgta
     2521 cagacaggac tgaccggaaa tgtggatgcg gcagtagccg ctactgaccg gataccgctt
     2581 cgcccggtgg tgtctgccgg tgaaccggtc attctgttgc cggggcagca gttcgaagtc
     2641 agtacacctc agggcagtat ccatgttgcc gggccggata cccgtctgcc ggacagcagt
     2701 ctgtttaaaa caaatccggc ggtgaatgta ccatatctgg ttgaaacgga cccgcgtttt
     2761 acgaaccaga agatgtggct gggcagtgat tacatgcaga aggcgttttc tcagaacggg
     2821 gataacatgc tcaaacgtct gggagacggt ttttacgaac agcgtctgat tcgtgagcag
     2881 gttgtctccc tgaccgggca acggtacctg gacggatacg gcagtgatga agagcagttt
     2941 aaggcattaa tggatgcggg tattgtattt ggtaagcaat acaacctgaa accgggtgtg
     3001 gcgctgactg ctgagcagat ggcgttactg accggggata ttgtctggct ggttaacacc
     3061 acggtgacac tgccggacgg cagcacgcag acggttcagg tacctcaggt gtatgcccgt
     3121 gtaaaacccg gtgatgtgaa cagtgccggt gcgctgattg ccggcaggga catggtgatg
     3181 aagctggatg gtgacctgtt taacagcggt aaactggccg ggaagcagac cgtgcagctc
     3241 agtgcagaaa acattcacaa ccaggccggc accattcagg gagcaaatgt cagcctgacg
     3301 gcccggacgg atattaacag taccggcggg ctgctgcagg ctacggacag cctgctggcg
     3361 atggccggac gggatatcag tctgaccacc actacccgta cggcacagcg cgacgccggg
     3421 cagaaccatt ttgaacgtac cagtatcgac agtgtggccg gcgtgtatgt gcagaacgac
     3481 cagggaagac tggtgctgca ggccggacgg gatatgaatc tgacggctgc aacggtggtg
     3541 aatcagggta agaacagcct gacgcaactc agtgcgggca gggatatgac gctttccaca
     3601 gtgaccacat ccgcgcagga caatattatc tgggataaaa ataatcgtct gtcgcagggc
     3661 gtcacacaga gtacgggcag cacgcttgcc ggtaacggtg atgtcacgct gaccgccgga
     3721 cgggatatga cgtcacaggc ggcatcgctg tcagctcaga aaggcctggc cctgatggcg
     3781 ggtcatgacg tgacgctgac cggtgcacag aataccagct cgctggatga ataccataaa
     3841 gtgaccggca gcagcgggat gttatcgaaa accaccacaa ccacgcatga tgtcactgac
     3901 cgccggacca tgacgggcag tgaactgaac ggggatacgg tcagtatcag tgccggacat
     3961 aatctgaacg tcacgggcag cagtgtggcc ggggataacc gtgtctccct ggctgccgga
     4021 aatgacctga gtatcggcac gctgacagaa agcaaccgtg aaacacacct gaaacaggag
     4081 aaaaaatccg ggctgatgag ctccggaggt gtgggattca gtgtgggcag ccagagtctg
     4141 aaagtcacgg acaccgcaac agacagcaca cagaaaggca gcacggtggg gagtgttcac
     4201 ggtgatgttt cccttcaggc aggtaaccgg ctgagcgcca acagtgctga cctgattgcc
     4261 ggcagggaca tggcactcag tggtaaggag gtcagcatta gcgccgtcac agaccggcat
     4321 gtgcagacgc atacggtgga gcaaaaaacc tccggtctga cgcttgccct gtccggcaca
     4381 gtgggcagcg ccctgaacac gacggtggag accgtacagg cggcgaaatc tgccggaaac
     4441 agccggcttg aagccctgca gggggtgaaa gccgcgctct cgggtgctca ggccgtgcag
     4501 gccggacgac tggcagaagc acagggcaac cagaaagaga ataacaatgc cgtgggcatc
     4561 agcctctctt acggcagcca gtcttcgaaa tcagaacaac gggcagagca gacggtggcg
     4621 aagagcagcg ccctgacagc aggtaataac ctcagtattc aggccaccgg ctccggcgtg
     4681 aagg
//
LOCUS       DAZKGF010000260         4666 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZKGF010000260 DAZKGF010000000
VERSION     DAZKGF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.260"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>4666
                     /locus_tag="ACHOQV_004765"
     CDS             <1..>4666
                     /locus_tag="ACHOQV_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFP9633013.1"
                     /translation="QDGVLSNRNTKTRGGISSAGTLTVRAGMLNNQQGFMVGQKDMTL
                     NTGTLDNRQGVLGSQASLQISSGTLMNQKGALKAGTDMLLSGGDVSNQEGTLVAGGDL
                     NTHLNVLENQQGTVVSNGNSRLDVTRFDNQGGRLVAQQSLTLSSTDIINDASGLIQSG
                     ASLNLRADTLSNRNSGDRGGVISQGPMTLNAGTLDSTAGVLLSGDALSLTAGVVNNTS
                     GQVVANGLLGWNSQALNNQSGLIQGKGISINTAGQTLDNRGGTLNSLQELTVSTGAMD
                     NRSGTVGAKTTADLSTTSLDNREGGRLVSEGELRLHTGGLQNSHGQIQSVGDILFDSV
                     RGVVDNVSGLIRSGSAITLNALQFINRHTQNTGQGLEAQTIHITTQDLDNQEGSILAD
                     RALTVMADRTLSNNDGVLSSGATLSVSGRQLAFSNRDGVVKAGQSVSVDAGQLGGDGK
                     LLSLGDMTLKSNTTFSNSGQTIANGNLTLSVNGDVSNTGSLLAGSRLDLNSIRLENTE
                     KGEISAGQTWLNVTDTLLNRGLIDGKYTHLQANTLTNSGTGRIYGDAVGVSAATFNNL
                     DENGVAAVLAGRERVDLGVQTLNNWTHSLIYSAGDMHTGGMLDANGAATGKAGVLNNH
                     SATIEAAGYLVLSAGQINNVNDHFTTERVVVSTEKVTEYQLSGSDKRWSAGEPGVYVD
                     NDSSNSLKKLHTPEGARDKFTQYDYTRTVEETRVKESDPGKILSGAGMTIVADKLFND
                     KSQVVAGGKLTIPSGNVENISVSGEQHVTDKGTSTYYYRIKKKGTDKQGESISPYEPP
                     TAIQTITLKPGELTSHGQVQGSQVTLSPLKPQGTDVQTGLTGNVDATVAGTDRIPLRP
                     VVSAGEPVILLPGQQFEVSAPQGSIHVAGPDTRLPDSSLFKTNPAVNVPYLVETDPRF
                     TNQKTWLGSDYMQKAFSQNGDNMLKRLGDGFYEQRLIREQVVALTGQRYLDGYSNDEE
                     QFKALMDAGIAFGKQYNLTPGVALTAEQMALLTGDIVWLVNTTVTLPDGSTQTVQVPQ
                     VYARVKPGDVNSAGALIAGRDMVMKLDGDLFNSGKLAGKQTVQLSAENIHNQAGTIQG
                     ANVSLTARTDINSTGGLLQATDSLLAMAGRDINLTTTTRTAQSDAGQNHFERTSIDSV
                     AGVYVQNDQGRLVLQAGRDMNLTAATVVNQGKDSLTQLSVGRDMTLSTVTTSAQDNIT
                     WDKNNRLSQGVTQSTGSTLAGNGDVTLTAGRDMTSQAASLSAQKGLALMAGHDVTLTG
                     AQNTSSLDEYHKVTGSSGMLSKTTTTTHDVTDRRTMTGSELNGDTVSIGAGHNLNVTG
                     SSVAGDNRVSLAAGNNLNIGTLTESNRETHLKQEKKSGLMSSGGVGFSVGSQSLKVTD
                     TATDTTQKGSTVGSVHGDVSLQAGNRLTVNGSDLIAGRDMALSGKEVSITAATDQHVQ
                     THTVEQKTSGLTLALSGTAGSALNTTVETVQAAKSAGNSRLEALQGVKAALSGAQAVQ
                     AGRLAEAQGNQKENNNAVGISLSYGSQSSKSEQQSEQTVAKGSILTAGNNLSIQATGS
                     GAK"
BASE COUNT         1177 a         1174 c         1451 g          864 t
ORIGIN      
        1 caggacgggg tacttagtaa ccggaacacg aaaacacggg gtggtatcag tagcgccggt
       61 accctgacag tgcgggctgg tatgctgaat aaccagcagg gatttatggt gggtcagaag
      121 gatatgaccc tgaacaccgg aacgcttgat aaccgtcagg gcgtgctggg gagtcaggcg
      181 tcattacaga tatcgtcagg tacgctgatg aatcagaaag gtgcactgaa ggccgggacg
      241 gatatgttgc tgagtggtgg cgatgtcagt aatcaggaag ggacgcttgt cgccggcggg
      301 gacctgaata ctcacctgaa tgtgctggaa aatcagcagg ggacggtggt cagcaacggt
      361 aacagcaggc tggatgtcac acggtttgat aatcagggcg gcagactggt ggcacagcag
      421 tcactgaccc tttcctcgac ggatatcatc aacgatgcca gcggcctgat acagagcggt
      481 gcatcgctga atctccgtgc tgacacgctg agcaaccgta acagtggtga ccggggcggg
      541 gttatcagtc aggggccgat gacgctgaat gccggcacac tggacagcac tgccggtgtg
      601 cttttgtcgg gagatgctct gtccctgact gcaggcgtgg ttaacaatac atccggtcag
      661 gtggtggcaa acggtctgct cggctggaac agccaggcgc tgaacaatca gtccggtctt
      721 attcagggga aaggcatcag cattaatacc gcaggccaga cgctggataa cagaggcggt
      781 acgctgaaca gcctgcagga actgactgtc agtaccggag caatggataa ccggagcggg
      841 accgtggggg cgaaaacgac agctgacctg agcaccacct cgctggataa ccgtgagggt
      901 ggccggctgg tcagtgaagg tgaactccgt ctgcatacgg gaggcctgca gaacagccac
      961 ggacagatac agtctgtggg cgacattctg ttcgacagtg tacggggcgt tgtggacaac
     1021 gtctctggtc tgattcgcag tggttctgcc attacgctga acgccctgca gtttatcaat
     1081 cgccacacgc agaataccgg ccaggggctg gaagcgcaga caatacacat cacgacgcag
     1141 gaccttgata accaggaggg cagtattctg gcagacaggg cgcttacggt tatggcagac
     1201 aggacgctca gtaataatga tggcgtgctc tcttccggcg cgacattgtc tgtcagtggc
     1261 cgtcagctgg cgttttcgaa ccgtgacggc gtggttaagg ccgggcagtc agtatctgtg
     1321 gatgccggtc agctcggtgg tgacgggaaa ctgctttcgc tgggcgatat gacgctgaaa
     1381 agtaacacaa cgttcagcaa cagtggtcag actattgcga acggaaacct tacgctttcc
     1441 gttaatggcg atgtctctaa taccggcagc ctgctggcag gcagccggct tgacctgaac
     1501 agcatccgcc ttgaaaacac agagaaaggt gaaatcagtg ccgggcagac ctggcttaac
     1561 gtgacggaca cactgctgaa ccgtgggctg attgacggga aatatacgca tcttcaggcg
     1621 aacaccctga cgaattccgg taccgggcga atctacggcg atgctgttgg tgtcagcgct
     1681 gccacattta ataatctgga tgaaaatggc gtggcggcgg tgctggccgg gcgtgagcgt
     1741 gtggacttag gggtacagac cctgaataac tggacgcaca gcctgattta cagtgcaggt
     1801 gatatgcata ctggtggcat gctggatgcg aatggtgccg ctacggggaa agccggtgta
     1861 ctgaataatc acagtgccac tattgaagcc gccggatatc tggtactgag tgccggacaa
     1921 atcaataacg tcaatgacca tttcaccacg gaacgcgtgg tggtttccac ggaaaaggtg
     1981 actgagtatc agctcagcgg ctcagataaa cgctggagcg ccggtgaacc gggcgtttat
     2041 gtggataacg attccagtaa ctcactgaaa aaactgcata caccggaagg cgccagagat
     2101 aaattcacgc agtacgatta cacccgtacc gtggaagaaa cccgggtaaa agaatccgac
     2161 ccgggaaaaa ttctgtccgg tgccggcatg acgattgtgg cagacaaact gtttaatgat
     2221 aagagtcagg ttgttgcagg cggtaaactg accattccgt caggaaacgt ggaaaacatc
     2281 agtgtcagcg gagagcaaca tgttactgac aaaggcacat caacatatta ctaccgcatc
     2341 aaaaagaaag ggacggacaa acagggggaa agcatttccc cttacgagcc gcccactgcc
     2401 attcagacca tcacgctgaa gccgggagag ctcaccagcc acgggcaggt tcagggcagc
     2461 caggtcacgc tttctcccct gaagccacag ggaacggatg tacagacagg actgaccgga
     2521 aatgtggatg cgacagtagc cggtactgac cggataccgc ttcgcccggt ggtgtctgcc
     2581 ggtgaaccgg tcattctgtt gccggggcag cagtttgaag tcagcgcacc tcagggcagt
     2641 atccatgttg ccgggccgga tacccgtctg ccggacagca gcctgtttaa aacaaatccg
     2701 gcagtgaatg tgccatatct ggttgaaacg gacccgcgtt ttacgaacca gaagacgtgg
     2761 ctgggcagtg attacatgca gaaggcgttt tctcagaacg gggataacat gctcaaacgc
     2821 ctgggggatg gtttttacga acagcgtctg attcgtgagc aggttgtcgc tctgaccggg
     2881 caacggtacc tggacggata cagtaatgat gaagagcagt ttaaggcgtt aatggatgcg
     2941 ggtattgcat ttggtaagca atacaacctg acaccaggtg tggcgctgac tgctgagcag
     3001 atggcgttac tgaccggtga tattgtctgg ctggttaaca ccacggtgac actgccggac
     3061 ggcagcacgc agacggttca ggtacctcag gtgtatgccc gtgtaaaacc cggtgatgtg
     3121 aacagcgccg gtgcgctgat tgccggcagg gacatggtga tgaagctgga tggtgacctg
     3181 tttaacagcg gtaaactggc cgggaagcag accgtgcagc tcagtgcaga aaacattcac
     3241 aaccaggccg gcaccattca gggagcaaat gtcagcctga cggcccggac ggatattaac
     3301 agtaccggcg ggctgctgca ggccacggac agcctgctgg cgatggccgg gcgggatatt
     3361 aatctgacca ccaccacccg cacggcacaa agtgacgccg gacagaacca ttttgaacgc
     3421 accagcatcg acagtgtggc gggcgtgtat gtacagaatg accagggaag actggtgctg
     3481 caggccggac gggatatgaa tctgacggct gcaacggtgg tgaatcaggg taaggacagc
     3541 ctgacgcaac tcagtgtggg cagggatatg acgctttcca cagtgaccac atccgcgcag
     3601 gacaatatta cctgggacaa aaataatcgt ctgtcgcagg gcgtcacaca gagtacgggc
     3661 agcacgcttg ccggtaacgg tgatgtcacg ctgaccgccg gacgggatat gacgtcacag
     3721 gcggcatcgc tgtcagctca gaaaggcctg gctctgatgg cgggtcatga cgtgacgctg
     3781 accggtgcac agaataccag ttcgctggac gaataccata aagtgaccgg cagcagcggg
     3841 atgttatcga aaaccaccac taccacgcat gatgtcactg accgccggac catgacaggc
     3901 agtgagctga acggggatac ggtcagtatc ggtgccggac ataatctgaa cgtcacgggc
     3961 agcagtgtgg ccggggataa ccgtgtctcc ctggctgccg gaaataacct gaatatcgga
     4021 acgctgacag aaagcaaccg tgaaacacac ctgaaacagg agaaaaaatc cgggctgatg
     4081 agctccggag gtgtgggatt cagcgtgggc agccagagtc tgaaagtcac ggacaccgca
     4141 acagacacca cacagaaagg cagcacggtg gggagtgttc acggtgatgt ttcccttcag
     4201 gcaggtaacc ggttgaccgt caacggctct gacctgattg ccggcaggga catggcactc
     4261 agcggtaagg aggtcagcat taccgccgcc acagaccagc atgtacagac gcatacggtg
     4321 gagcagaaaa cctccggtct gacgcttgcc ctgtccggca cagcgggcag cgccctgaat
     4381 acgacggtgg agaccgtaca ggcagcgaaa tctgccggaa acagccggct tgaagccctg
     4441 cagggggtga aggccgcgct ctcgggggct caggccgtgc aggccggacg actggcagaa
     4501 gcacagggca accagaaaga gaataacaat gccgtgggta tcagcctctc ttacggcagc
     4561 cagtcttcga aatcagaaca gcagtcagag cagacggtgg cgaagggcag tatactgacc
     4621 gcaggtaata acctcagtat tcaggccacc ggctccggcg cgaagg
//
LOCUS       DAZKGF010000261         4617 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZKGF010000261 DAZKGF010000000
VERSION     DAZKGF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.261"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(149..436)
                     /locus_tag="ACHOQV_004766"
     CDS             complement(149..436)
                     /locus_tag="ACHOQV_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFP9633014.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLFIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(511..654)
                     /gene="mgrB"
                     /locus_tag="ACHOQV_004767"
     CDS             complement(511..654)
                     /gene="mgrB"
                     /locus_tag="ACHOQV_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFP9633015.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            813..1052
                     /locus_tag="ACHOQV_004768"
     CDS             813..1052
                     /locus_tag="ACHOQV_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFP9633016.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1196..1987)
                     /gene="kdgR"
                     /locus_tag="ACHOQV_004769"
     CDS             complement(1196..1987)
                     /gene="kdgR"
                     /locus_tag="ACHOQV_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFP9633017.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2164..3537
                     /locus_tag="ACHOQV_004770"
     CDS             2164..3537
                     /locus_tag="ACHOQV_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9633018.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3583..4464)
                     /gene="htpX"
                     /locus_tag="ACHOQV_004771"
     CDS             complement(3583..4464)
                     /gene="htpX"
                     /locus_tag="ACHOQV_004771"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFP9633019.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
BASE COUNT         1139 a         1180 c         1029 g         1269 t
ORIGIN      
        1 gatatcccgt tacgaaaata aataatatga taattatttg aagtcagacc agagttttaa
       61 tttgacgcag ttgcaccatg acagtgcgta taatcaggat gaattgagta acccttcaac
      121 gacggggtta ctcaagattg aggaaagatt atcgttcggc gaccagacga ataatatctt
      181 cgtcttcaac cgatttttga ctctcaacgg tctttttatc tgctttctct ggttcgtttg
      241 cttcgcacaa acgacgtaac agagcatttt gacgtttttg ctgatccagc aacgcctcaa
      301 gcagttcaat ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac cacaagataa
      361 gcccgataaa aagaaccacc accgatacaa ccaaagacgc aatgttcatc acgcctgaat
      421 ttacaacttc gttcatttca ccacctctga gtagaggcgc tattctacca ctgctggaga
      481 ggaagaaaat ctagtgctga aaaaatgata tcaccacggg ataaactggt taatggcaca
      541 aattccgctg aaaaattgta catcctgatc gcacatcatg ttgaatacct gcgcccaaag
      601 cagcaagcaa gccaacacca cgacaaccag aacgacccat cgaaactttt tcactccaat
      661 ctccgtttca cctaccttat gtcattaaac tagcatgcgt tagttaaaca gcgcctaact
      721 atgtcgatat tcgtgctttt tcagaatgag tcgcttgctt cagcgcatat tgccgctacg
      781 attaagcgaa cataaaaaga gaagaggttg taatgcgatt catcattcgc acagttatgc
      841 tgatagcact ggtatggata ggattattac tcagcggata tggcgttttg attggaagca
      901 aagagaatgc cgcgggatta ggtttgcaat gtacctatct gaccgccaga ggcaccagca
      961 cggtacaata cctgcacact aaaagtggtt ttctggggat aacagattgc ccgttgttac
     1021 gtaaaagcaa tattgtggtc gataatggtt aagggtaata aataaccaga ttctcactca
     1081 tgagtaaaat attcttatcg caagtgcgtt taatataacg ccaccttcaa ctttattttt
     1141 ctcatgctaa ataaagaacc tgtctgtttg atattcagac aggttcttac tctcatcaga
     1201 acggatagtc gtgataaccc atttgggcag aaattttgcg cgccgcggta tgcaacattg
     1261 cgacatattc ctgtaaacgc tcttcagaga aacgtaacgt cgggaaggag atgctcaaac
     1321 cggcaatgac cacgccaaag cgatcaaata ccggaaccgc aatgcatcgc agcccttctt
     1381 cctgctcttc gttatcttcg ccatacccct gctcgcgcac ctggtccaga acgggtaata
     1441 acgcttctgt actggtgatg gtccgctcgg tactgcgttt atactccacg ccctcaagaa
     1501 tttgcttcac ttcatcgcga tcgcgccatg ccagcagtac cttaccaatc gcggtgctgt
     1561 acagcggatt acgacgccca atccgtgaat acatgcgcaa attgtacata gagtcaattt
     1621 tgtgaatgta aacaatactg tcttcgtcca gtgcgccgag gtggatagtt tctttggtca
     1681 ggcgggagat ctcacgcatc tggatatctg cgctacgaat taaatcgacg ttttgtaacg
     1741 cgcgagcgcc cagttcaaac aatttcaggg tcagcgaata tttctccgac tccccttcct
     1801 gcgccacata acctaaggtt ttcatggtct gtaaaaagcg ataaacggtg ctttttgaca
     1861 tcatgacgcg ctgcgacaac tcggttatcc ctatttcgcg ctcttcaccc agcgcctgca
     1921 aaatgccaaa aacttttagc acggaagata cagaatcagg ctgtttatcc agatctgcgt
     1981 tagccattta tcacctcatt gcgagtgttt tataaaaatc agaactgttt tttattataa
     2041 tttcgcacca gggtggtcgc aatccatctt ttgccggtta gttacaattc tgcgacatcc
     2101 accgtgaata tcagtgctag aatcataccc ctgttgatta ttcaccaaag atataaaatt
     2161 cctatgccaa aagttcaggc cgacggcctg ccattgcccc agcgatacgg tgcgatatta
     2221 accattgtga ttggtatttc gatggctgtc cttgacggcg caatcgccaa cgtcgccttg
     2281 ccaacaatcg ccacggacct tcatgccacg ccagccagtt ccatctgggt agtgaacgcc
     2341 tatcaaatcg ccattgtcat ctccctgctc tcgttttcgt ttctgggcga tatgtttggc
     2401 tatcgacgta tttataaatg cggtctggtc gtttttctgt tgtcttcact attctgcgcc
     2461 ctttctgatt cgctgcaaat gctcaccctt gcgcgtgtca tacaaggttt cggcggtgca
     2521 gcgttgatga gcgttaatac cgcacttatc cgcctgatct atccacaacg ttttctgggt
     2581 agagggatgg gcataaactc gtttattgtt gccgtttctt ctgctgccgg gccgacaatt
     2641 gctgcagcaa tcctctccat cgcatcctgg aaatggttat ttttaatcaa cgtaccgttg
     2701 ggtattatcg ccctgcttct ggcgatgcgt tttctgccac ccaatggttc tcgcgccagt
     2761 aaaccccgtt tcgacctgcc cagcgccgtg atgaacgcgt taaccttcgg cctgcttatt
     2821 actgcattga gtggtttcgc tcaggggcaa tcgctgacat tgattggtgc ggaactggtg
     2881 gtaatggttg tcgttggtat tttctttatt cgccgccagc tttctcttcc cgtaccgctg
     2941 ctaccggtgg atttactgcg tatcccgctg ttttcacttt ctatttgcac atctgtttgc
     3001 tctttctgcg cacaaatgct ggcaatggtt tcccttccct tttacctgca aaccgtgctc
     3061 gggcgtagtg aagtcgaaac aggtttactt ctgacaccgt ggccgttagc aacaatggtg
     3121 atggctccac tggcaggcta tttgattgaa cgcgtacatg caggattgct gggtgcttta
     3181 gggttattca tcatggctgc ggggcttttt tccctagttc tgctgccagc gtcacctgcg
     3241 gatatcaata ttatctggcc gatgatctta tgtggtgctg gatttggctt gttccagtca
     3301 cccaataacc acaccattat tacctccgct cctcgcgaac gtagcggtgg agccagtggc
     3361 atgttaggga cggctcgtct tctgggtcag agtagcggcg cggctctggt ggcgctgatg
     3421 ctaaatcagt ttggtgataa tggtacgcac gtctcgctga tagctgcggc tattctggcg
     3481 gtgattgcag cctgtgtcag tggtttacgt atcactcagc cacgatccag ggcataaaaa
     3541 aagcgcgtcg atcaggacgc gctttttagt atttacttca tattacttca ggtattcacc
     3601 cgtacgcaga gcttcaatac gtttatccag tggcgggtga gtcatgaaca actcactgag
     3661 cgatttcgac ttcccgttaa tgcagaaagc catcatgctg gttgcttctt gcggttcata
     3721 gctggttttc aggcgctgca atgcagcaat cattttctcg cgaccaacca gttttgctga
     3781 acccgcatcg gcgtggaatt cacgatgacg cgagaaccac atggtgataa tgctcgccag
     3841 aataccaaac accagttcca gaaccgttgc aaccgcaaag tagatcagcg ggttgccgtt
     3901 gctctcttca ccttcatcac gatttccgcc cataaaaccc gcggcaagct gcgccagaat
     3961 acgggaaata aagataacga aggtgttcac cacgccctga atcagcgtca tggtgaccat
     4021 atcaccattg gcgatgtggc tgatttcgtg agcaattacc gcctcggctt catccgggct
     4081 catgttctgc agcaaaccgg tgctgacagc aaccagagag gcatcacggc gcgcaccggt
     4141 tgcaaaagca ttgatgtccg gcgcatggta gatagccact tgcggcatag cgatccccgc
     4201 ctgacgagcc tgggttgcta ccgtattgac cagccaacgt tccctttcgt tacgcggttg
     4261 ctcgatcact tccccgccaa cagatcgtaa tgccatccat ttggacatca gaagcgaaac
     4321 gaaggaacca ccaaaaccga acagcaaggc catgatcatc agcccctgaa cgctgctcga
     4381 ctgtatccct gtcaggctca gtaccagccc gaaaacgacc attacggcca ggttcgttag
     4441 caggaagagc gcgattcgca tcataatttt ctttttacct cagtttaaca aaacgcaata
     4501 tgcgataccc acatcgtatg ggttacgcga ctattttcaa gtctggatag tgcgtaagtc
     4561 accagaaaga cacaacttta cattttgtag catctgattt acggcatctt gctgctg
//
LOCUS       DAZKGF010000262         4596 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZKGF010000262 DAZKGF010000000
VERSION     DAZKGF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.262"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHOQV_004772"
     CDS             complement(<1..593)
                     /locus_tag="ACHOQV_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9633020.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(708..783)
                     /locus_tag="ACHOQV_004773"
     tRNA            complement(708..783)
                     /locus_tag="ACHOQV_004773"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Thr,seq:ggt)
     gene            complement(790..864)
                     /locus_tag="ACHOQV_004774"
     tRNA            complement(790..864)
                     /locus_tag="ACHOQV_004774"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Gly,seq:tcc)
     gene            complement(981..1065)
                     /locus_tag="ACHOQV_004775"
     tRNA            complement(981..1065)
                     /locus_tag="ACHOQV_004775"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Tyr,seq:gta)
     gene            complement(1074..1149)
                     /locus_tag="ACHOQV_004776"
     tRNA            complement(1074..1149)
                     /locus_tag="ACHOQV_004776"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Thr,seq:tgt)
     gene            1511..2461
                     /gene="coaA"
                     /locus_tag="ACHOQV_004777"
     CDS             1511..2461
                     /gene="coaA"
                     /locus_tag="ACHOQV_004777"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9633021.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHOQV_004778"
     CDS             complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHOQV_004778"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFP9633022.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLTPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHOQV_004779"
     CDS             complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHOQV_004779"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP9633023.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAKGQSVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCIGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPSTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1181 a         1154 c         1044 g         1217 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgccccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgc
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcgc gagcagcacc gccgtaggtt
      481 ttagccagta cggtagtgat tgcagcggtc agcgttgttt taccatggtc aacgtggccg
      541 atagtaccga cgttaacgtg cggttttgta cgttcaaact tttctttaga catcgattgt
      601 ccctctaaga cacggataaa tcggtgatat caccacatca accaggcaac atgcccgact
      661 tgttgaatgc aataaacaga aggaaaaaac agggaggaga aaagaagtgg tgctgatagg
      721 cagattcgaa ctgccgacct cacccttacc aagggtgcgc tctaccaact gagctatatc
      781 agcacatctt ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      841 taatagccat tatacgatgc ccgcatcctg gaactcggct acctgatttt cattctgcac
      901 tgaatatcga gagaagctct ctttattcga gccggtaagc gaacttatcg tctcgggcta
      961 cgccatcgcg tggccgaaat tggtggtggg ggaaggattc gaaccttcga agtctgtgac
     1021 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccggact tgatggtgcc
     1081 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
     1141 taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc aactaaaaaa
     1201 ttgcataatt tgttttattg gtcacatttt atgcgacacg atgaagaaac agccgaacgg
     1261 agggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgtgctc ctgctaaacc
     1321 ataattcttt ttatcagatg gaatatctgt cacattgctt ttcaacgata gcttcctggg
     1381 agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca ttaacccatt
     1441 caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta tgaccgcctg
     1501 aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac agtttgaccg
     1561 caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag atgagatcgc
     1621 ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga tctatttacc
     1681 tctgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg cagttctgga
     1741 acagtttctt ggtaccaacg ggcaacgcat tccttacatt atcagtattg ctggtagtgt
     1801 cgcggtgggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc gttggccgga
     1861 acatcgtcgt gttgaactga tcactacaga tggcttcctt caccctaatc aggttctgaa
     1921 agaacgtggt ctgatgaaga agaaaggctt cccggaatcg tatgatatgc atcgcctggt
     1981 gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcccctg tttactcgca
     2041 tcttatttat gatgtgatcc cggatggaga taaaactgtt gttcagcctg atattttaat
     2101 tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc cacatcatgt
     2161 atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag acttacttca
     2221 gacgtggtat atcaaccgtt ttctgaaatt ccgcgaaggg gcttttacag acccggattc
     2281 ctattttcat aactacgcga aattaactaa agaagaagct attaagactg ccatgacatt
     2341 gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc gcgagcgtgc
     2401 cagtttaatc ctgacgaaaa gtgctaatca tgcagtagaa gaggtcagac tacgcaaata
     2461 atttaaaggg gagttaatgc tcccctttct tatttttctg cactacgcag ggatatttca
     2521 ccgcccatcc agggttttat tattccatcc tgctcaagta ataaagcacc ctgtttgtct
     2581 attccgcgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac tgggcgatta
     2641 ataaaattat ccaacttttc ccagcgcgaa agataaggtg tcaatccttc ttgctcgaag
     2701 agttccagcg ccgcgcgtaa ttcacgtatc agcatggccg caagagtatt acgatcgaga
     2761 ttgatcccgg cttcctgcag cgtgatccac ccttgattaa cgacactctc ttcaacacga
     2821 cgcattgcca tgttgatccc tgcaccaatg actatttgcg ccgcatcgcc ggttttgcca
     2881 gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc attaggccat
     2941 ttaacacgaa ctttatctgc acccagcttg cgtaatactt ccgccatcac gataccgata
     3001 accagactta aaccaatcgc cgccgccggg ccctgttcca gacgccagaa catcgacaaa
     3061 tataagttcg cgccaaaagg cgaaaaccat ttccgaccac gacgaccacg gccagcatgc
     3121 tggtattctg caacacaggc atcgccagat ttaagctctc cgatgcggtc caggagatac
     3181 tgattcgtgg agtcaataac tggcagcacg gctacactgc cgtcatccag ctgactcaat
     3241 atcttttcgg cattaagtaa ctggattggc tcgggcaagc tgtatccttt acccggaaca
     3301 gtaaatacat caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg
     3361 ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcgccatt cgctaacagg
     3421 gcgatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact gcgctcactt
     3481 cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat ttttcaccca
     3541 ctttttgtct tacatgatgc gccagctgta caacatcttc gctttttgca tcgtgctcat
     3601 ttatgagaac cagcgcctgt tgacggtgta ccgcagcccc accaatttgc gtccctttaa
     3661 gctggcactg atcaataagc caacctgcag ccagttttac tgaaccatcc acctgggggt
     3721 aatgtggggc tgttggaaat tgtgccaaca acgcgttagc agtttcggca gaaacaacag
     3781 gatttttgaa gaaactaccc gcattaccgt tcacttttgg atcagggagt ttggtggtgc
     3841 gcatatgaca caccgcatca aatacttgct gtggcgttac cgttgaagga tccaggcgag
     3901 ttaagtcacc atacgtcagt acaggttgcc actcttttgg cagacgcaga cctacggcta
     3961 caatggcgaa gcgatcctgg tattcatgtt taaaaatact gtctcgatag ccaaaacggc
     4021 actctttggc agttaagcgt acttgcttgc ctgtcgccag ttcaacacaa tcaacataag
     4081 cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt gatgagccga
     4141 tacaaccagg aattaatgcc agattttcca gaccaggcat accttcctgc aaagtgtatt
     4201 ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca tcaggttcat
     4261 catgaatttc gatacctttg atccggttga tgatcaccgt accgcgatag tcttccagaa
     4321 aaagtacatt acttccttca cccagaataa gaacggattg tccttttgcg gttgcatgct
     4381 gccaggcatt gagtagttgt tgttcgtctt cggcacatac aatgtgctga gcattatgat
     4441 caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc ctgatgcaaa
     4501 aacgaggcta gtttaccgta tctgcggggg gatggcttgt agatatgatg acaggaagag
     4561 tttgtagaaa cgcaaaaagg cccagtcttc cgactg
//
LOCUS       DAZKGF010000263         4510 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZKGF010000263 DAZKGF010000000
VERSION     DAZKGF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.263"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            221..1378
                     /gene="acrE"
                     /locus_tag="ACHOQV_004780"
     CDS             221..1378
                     /gene="acrE"
                     /locus_tag="ACHOQV_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFP9633024.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1390..4494
                     /gene="acrF"
                     /locus_tag="ACHOQV_004781"
     CDS             1390..4494
                     /gene="acrF"
                     /locus_tag="ACHOQV_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFP9633025.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTAHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASQLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
BASE COUNT         1101 a         1092 c         1187 g         1130 t
ORIGIN      
        1 acgaggattt gattctacct ctttaaaccc gcgtcaaaat aaaacagtag aatattaatc
       61 tttttttgtg tttatgtgcc ttgagacgcc agtattcata actattcctt acatcgacga
      121 atgataattt gtaggatagc gaactgtatt tttctttctg cgagttaacg cgttgccttt
      181 ttgggtaaat aacgcgcttt tggttttttg aggaatagta atgacgaaac atgccaggtt
      241 tttcctcctg ccctccttta ttctgatctc cgcggcttta atcgccggtt gtaacgataa
      301 gggagaagag aaagctcacg tcggtgaacc gcaggttacc gttcatattg tgaaaacggc
      361 cccgttagaa gttaagactg aattaccagg ccgcaccaat gcttaccgta tagccgaagt
      421 tcgcccacag gttagcggga tcgtactgaa tcgcaatttc actgaaggca gcgatgtgca
      481 agcaggccag tccctgtacc agatcgatcc cgcgacctat caggcaagtt atgacagcgc
      541 gaaaggcgaa ctagcgaaaa gtgaagccgc cgccgccatc gcgcatttga cggtaaaacg
      601 ttacgttccg ctcgtgggta cgaaatacat cagtcagcag gagtacgacc aggccattgc
      661 tgatgctcgc caggccgatg ccgccgtgat tgccgcaaaa gctacagttg aaagcgctcg
      721 catcaatctc gcttatacca aagtgactgc gcctattagc ggacgtatcg gcaaatcgac
      781 tgtgaccgaa ggcgctcttg tcactaatgg gcaaacgact gaactggcga ctgtccagca
      841 gctcgatcct atctacgttg atgtgaccca atccagcaac gattttatga ggctgaagca
      901 atccgtagag caaggaaatt tgcataagga aaacgccacc agcaacgtag agttggtcat
      961 ggagaacggt caaacctatc ccctgaaagg tacgctgcaa ttctccgatg tgaccgttga
     1021 tgaaagcacc ggctccataa ccctacgtgc tgtcttccct aacccgcaac atacgctttt
     1081 gccgggtatg tttgtgcgcg cacggattga tgaaggcgtc caacctgacg ccattcttat
     1141 cccgcaacaa ggcgttagcc gcacaccacg tggtgatgca accgtgctga ttgttaacaa
     1201 taaaagtcag gttgaagcgc gccctgtcgt tgccagccag gcgattggcg ataaatggtt
     1261 gattagcgaa gggctgaaat ctggcgatca agtcattgtc agcggcctac aaaaagcgcg
     1321 tccgggagag caggttaaag ccactaccga tacccccgca gatactgcat cgaagtaagg
     1381 caatctgaca tggcaaactt ttttattcga cgaccgatat ttgcatgggt gctggccatt
     1441 attctgatga tggcgggcgc actggcgatc ctacaattgc ccgtcgctca gtatccaacc
     1501 attgcaccgc ctgcggtttc tgtttcagca aactatccgg gcgctgatgc gcagaccgtg
     1561 caggatacgg tgacgcaggt tatcgaacag aatatgaacg gtatcgataa cctgatgtat
     1621 atgtcctcca ccagcgattc cgccggtagc gtgacaatta cccttacctt ccagtccggg
     1681 accgatcctg atatcgcgca agtgcaggtg cagaacaaac tccagctcgc cacgccgttg
     1741 ctgccgcagg aagtacagca gcaggggatc agtgttgaaa agtccagtag cagctatttg
     1801 atggtggcgg gctttgtctc tgataaccca ggcaccacac aggacgatat ctcggactat
     1861 gtggcctcta acgttaaaga tacgcttagc cgtctgaatg gcgtcggtga cgtacagctt
     1921 ttcggcgcac agtatgcgat gcgtatctgg ctggacgccg atctgctaaa caaatataaa
     1981 ctgacaccgg ttgatgtgat taaccagttg aaggtacaga acgatcagat cgctgccgga
     2041 cagttgggcg gaacgccagc gttaccaggg caacagttga acgcctcgat tattgctcag
     2101 acgcggttaa aaaatccgga agaattcggc aaagtgaccc tgcgcgtaaa cagtgacggc
     2161 tcggtggtac gcctgaaaga tgtcgcacgg gttgaacttg gcggtgaaaa ctacaacgtt
     2221 atcgctcgta tcaacggaaa accggcggcg ggcctgggga ttaagctggc aaccggcgca
     2281 aatgctctcg ataccgcgaa agccattaag gcaaaactgg cggaattaca gccattcttc
     2341 ccgcagggaa tgaaggttct ctacccttat gacaccacac cattcgtcca gctttctatt
     2401 cacgaagtgg taaaaacgct gttcgaagcc attatgctgg tgttcctggt gatgtatctg
     2461 ttcttgcaga atatgcgagc aacgctgatc cccaccattg cggtacccgt ggtgttgtta
     2521 gggacgtttg ccatcctcgc cgcttttggt tactccatca acacactaac gatgttcggg
     2581 atggtgcttg ccatcgggct gctcgtcgat gatgcgatag tggtggtgga gaacgtcgag
     2641 cgcgtgatga tggaggataa gctcccgcca aaagaagcga cggaaaaatc aatgtcgcaa
     2701 atccagggcg cactggtggg tatcgcaatg gtgctgtcag cggtatttat tccgatggca
     2761 ttcttcggcg gttctactgg ggcaatttat cgtcagttct ctatcaccat cgtttcggca
     2821 atggcgcttt ctgttctggt ggcattgatt cttacccctg cgttatgtgc aacgctgctt
     2881 aaacccgtct ctgctgagca tcacgaaaat aagggcggtt tcttcggctg gtttaatacc
     2941 accttcgatc atagcgttaa ccactacacc aacagcgtcg gcaaaatcct cggttccaca
     3001 ggacgatatt tactgatcta tgcactaatt gttgcaggaa tggtggtgtt gtttttacgt
     3061 cttccgtctt ccttcttacc tgaagaggat cagggtgtct ttctgactat gattcagtta
     3121 cccgctggcg cgacgcaaga gcggacgcaa aaagtactgg atcaagtgac ggattactat
     3181 ctgaagaacg agaaagcgaa cgttgaaagt gtctttacag ttaacggctt tagcttcagc
     3241 ggccaggcac aaaacgccgg tatggctttc gtcagtctga aaccgtggga agagcgtagt
     3301 ggtgacgaaa acagtgcgga agcggtaatc catcgtgcca aaatggaatt gggcaagatc
     3361 cgcgacggtt ttgtcattcc attcaatatg ccagccattg ttgaactggg cacggcaacg
     3421 ggtttcgact ttgagttaat tgatcaggct gggctgggtc atgatgccct gacccaggcc
     3481 cgtaaccagt tgcttggcat ggcggcgcaa catcctgcca gcttagtcag cgtgcgtcct
     3541 aatggtctgg aagacaccgc gcagtttaaa ctggaagttg accaagaaaa ggcgcaggca
     3601 ttaggtgttt cactttctga catcaatcag accatttcaa cagcgttggg tgggacttac
     3661 gttaacgact tcattgaccg tggtcgcgta aaaaaggtgt atgttcaggc ggatgccaaa
     3721 tttcgtatgc tgccagaaga tgtcgataaa ctttatgtcc gtagcgccaa tggcgaaatg
     3781 gtgccattct ctgcctttac cactgcacat tgggtgtatg gttctccgcg actggagcgc
     3841 tacaacggtc tgccgtcaat ggagattcag ggggaagccg cgccaggaac cagttccggc
     3901 gatgccatgg cgttgatgga aaaccttgca tcacaattac cagctggtat tggttatgac
     3961 tggacgggta tgtcatatca ggaacgctta tcgggaaacc aggctcccgc tctggtagca
     4021 atttcctttg tggttgtttt cctgtgcctt gctgcactct atgaaagctg gtcaattcct
     4081 gtctcggtta tgttggtcgt gccgttaggg attgtcggcg tgctactggc ggcgacactc
     4141 tttaatcaaa aaaatgacgt ctactttatg gtgggcttgc taacgacaat tggcttgtcg
     4201 gctaaaaacg ctatattgat tgttgagttc gctaaagatc tcatggagaa agagggtaaa
     4261 ggtgttgttg aagcgacact aatggcagta cgtatgcgtc tgcgtcctat cctgatgacc
     4321 tctctcgcct ttattctcgg cgtattaccg ctagctatca gtaacggtgc cggcagtggc
     4381 gcgcagaacg cagtgggtat cggggtaatg ggcgggatgg tttctgcaac gttgctggca
     4441 atcttcttcg tgccggtgtt ctttgtggtg atccgccgtt gctttaaagg ataaatcaga
     4501 aacataaagg
//
LOCUS       DAZKGF010000264         4509 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZKGF010000264 DAZKGF010000000
VERSION     DAZKGF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.264"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            46..300
                     /locus_tag="ACHOQV_004782"
     CDS             46..300
                     /locus_tag="ACHOQV_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633026.1"
                     /translation="MATTTKDMAEIASAYTIIIHRLIANNARNALNTAKPLWETKKDI
                     ISALQGLQKCADLAGDYAVYMAIEDTIEKIESGKSLSDFV"
     gene            321..563
                     /locus_tag="ACHOQV_004783"
     CDS             321..563
                     /locus_tag="ACHOQV_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFP9633027.1"
                     /translation="MDKEYKVLINKALERFNFRLSMSGVAAEHAARDSLTRAIRSLYD
                     AAFYADDLDALNELSELVRDAECGVHIEPYALGNAA"
     gene            560..685
                     /locus_tag="ACHOQV_004784"
     CDS             560..685
                     /locus_tag="ACHOQV_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633028.1"
                     /translation="MSVFLSWVILIISVLMAIGIMRIIEALKNIESLFTGEERRK"
     gene            765..1253
                     /locus_tag="ACHOQV_004785"
     CDS             765..1253
                     /locus_tag="ACHOQV_004785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633029.1"
                     /translation="MSINTPTEINKLFTDPAHIESTQKMLTEITNALDERISGIDKDN
                     HSSTYMTLKIQSMAMVIANQTISELYMKNLRLERELAELRAQMKTDESPTGEINARPE
                     HTNTHITMITRKAERTHICELLRELIETAKETHTCPQETCAAIERLIAILTNPDELKG
                     GA"
     gene            1253..1834
                     /locus_tag="ACHOQV_004786"
     CDS             1253..1834
                     /locus_tag="ACHOQV_004786"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9633030.1"
                     /translation="MNHLMIDTETLGNGPDAAIFAIGAVFFDPFTGKLGKQFERFIDP
                     VDSERNGGTVNAATAVWWAGQSVDARACLRNADGTELAAVTEFLAFISRNMHDESPGK
                     TLTVWCKGASFDFPILKSAITRTTGDKSIPWCYWNERCMRPLIDMAESTGWRMPGRQG
                     KETIAHTALNDAIYQAKVVSEIWQRFTAPFLSV"
     gene            1872..2846
                     /locus_tag="ACHOQV_004787"
     CDS             1872..2846
                     /locus_tag="ACHOQV_004787"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFP9633031.1"
                     /translation="MSKELLSGTPLKWVGGKSRIMNTLKAHLPEADCLVEPFVGGASV
                     FMNTSYSRYILGDSNGALINFWRAARDNTDSLIDEAKDLFREHNNRTDYLAIRNTFNA
                     ENHAFLSTRISDRRELRLAAMFLYLNRHCFNGLYRVNGKGDFNVPFGGYRTPYFPERE
                     IRAFADKANSTRTLLVHGDFRKTLSCASHLFSMGNTLCVYCDPPYLPCNGRDNFTAYG
                     KPFNEGEHIRLRATLDHLSQETDGMTCITISNSDTPETRRIYQGYRMNCIQAPRSVGA
                     RTREPAPEVIATLKQCDACGRHGGGYCPDCGPVTGDATYCVMFTPAST"
     gene            2882..3118
                     /locus_tag="ACHOQV_004788"
     CDS             2882..3118
                     /locus_tag="ACHOQV_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633032.1"
                     /translation="MNSERNKALLRGLRHIARAHEETAKVAAAVGKPKVVISSSISLG
                     ITMEAIRVIQDQEREIAALKKLLHGKSAGQGVKQ"
     gene            3115..3321
                     /locus_tag="ACHOQV_004789"
     CDS             3115..3321
                     /locus_tag="ACHOQV_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633033.1"
                     /translation="MIKESTVIRFTTHGHQYEVDESLIDQGMTRRDSRNPDIYHIRLI
                     NGSHFRATNVEEVRVLTTSCRRAL"
     gene            3658..3885
                     /locus_tag="ACHOQV_004790"
     CDS             3658..3885
                     /locus_tag="ACHOQV_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP9633034.1"
                     /translation="MANRRQQRARAERLHTRTEINRRLWRASRVAQIMYINMLHEHAH
                     ALSDRYCAAVFSYLADDLAELQDLINQQHHH"
     gene            3954..4370
                     /locus_tag="ACHOQV_004791"
     CDS             3954..4370
                     /locus_tag="ACHOQV_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633035.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRFNDWHDYYRRACKQAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGDGFGLFHINGTDSTAISWSSSEMTLVLEMTTHSV
                     AVKGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
BASE COUNT         1259 a         1211 c         1123 g          916 t
ORIGIN      
        1 ttaatcagcg gtcgcagttt tcagcatcca tgggggaatt aatacatggc aacaacgaca
       61 aaagacatgg cagagatagc atcagcctac acaattatca tacaccggct catagctaat
      121 aatgcccgta atgcactaaa tacagcaaaa cctttatggg aaacaaaaaa agatatcatc
      181 agcgcattac aggggctaca gaaatgcgcc gacctcgcag gcgattacgc cgtatacatg
      241 gctattgaag acacgataga gaagattgaa tcaggtaaat cattaagcga ttttgtttaa
      301 ttgttttccg gggaaataca atggacaaag aatataaggt tctcatcaat aaagcccttg
      361 agcgctttaa ctttcgcctg agtatgtcag gagttgccgc agaacatgca gcaagagact
      421 cactgaccag agcaattcgc agcctgtatg acgcagcttt ttacgctgac gatctggatg
      481 ctctcaatga actgtctgag cttgttcgtg atgcagaatg cggagtacat attgagccat
      541 acgcacttgg gaatgcagca tgagcgtatt tctttcatgg gtgattctga ttatttccgt
      601 gcttatggcg attggcatta tgcgaattat cgaagcgctg aaaaatatcg aatctttatt
      661 tactggcgaa gaacgccgta aataaataac caaccggaca gaaaacagta aaaccatccg
      721 catctgcgga gggcttcact caatccagaa acggagaaac agccatgagc atcaatacac
      781 ctacagaaat aaacaaacta tttaccgacc cggcgcatat tgaatcgacg caaaaaatgt
      841 tgactgaaat aaccaatgca ctggatgaaa gaatatcagg aatagataaa gacaatcatt
      901 cttctactta catgactctg aaaatacaaa gcatggcaat ggtgatagca aaccagacca
      961 tcagtgaact ctatatgaaa aacctgcgcc tcgagcgcga actggcagaa ctccgggcac
     1021 aaatgaaaac agacgaatcc cccaccggag aaataaacgc cagaccggaa catacgaaca
     1081 cgcatatcac catgatcaca agaaaagccg agcgtactca tatttgcgaa ttactgcgcg
     1141 agctgattga gacagcgaaa gagacacaca cctgcccaca ggaaacctgc gcagccattg
     1201 agcgactgat tgcaatactc accaacccgg acgaactgaa ggggggcgca taatgaacca
     1261 tctcatgatt gacaccgaaa cactgggtaa cggaccggac gcagccatat tcgcgatcgg
     1321 ggcagtgttt ttcgacccgt tcaccggaaa gctgggaaaa cagtttgagc gattcattga
     1381 cccggtggat tcagagcgca acggtggaac cgtcaatgca gcaaccgctg tatggtgggc
     1441 cggacaatcc gtggacgcca gagcctgcct gcgcaatgca gatggaacag agctggcagc
     1501 agtgaccgaa ttcctggcgt tcatttcacg caatatgcat gacgaaagcc cgggcaagac
     1561 actgaccgta tggtgcaagg gcgcttcgtt tgacttcccg atcctgaaat ccgctatcac
     1621 gcgaacaacc ggagataaaa gcatcccctg gtgttactgg aacgagcgct gtatgcgtcc
     1681 gcttatcgac atggctgaat caacaggctg gagaatgcca ggcagacaag gaaaagagac
     1741 cattgctcat accgcactga acgacgccat ttaccaggcg aaggttgttt cagaaatctg
     1801 gcagcgcttc actgcgccat tcctgagcgt atgaagcgga cacacgaaac ctgtaacaaa
     1861 gaggccgcgt tatgagcaaa gaactgttat caggcacacc actgaaatgg gtgggtggaa
     1921 aaagccgcat catgaacaca ctgaaggcgc acctgccaga agccgactgt ctggttgagc
     1981 cgttcgtggg cggcgcatcg gtattcatga acaccagtta cagccgctac atccttggag
     2041 attccaatgg tgcgctgatt aatttctggc gggcagcacg ggacaacacc gacagtctga
     2101 tcgacgaggc aaaagacctg ttccgggaac acaacaacag gactgactat ctcgccatcc
     2161 gaaacacctt taatgcagag aatcatgcat tcctgagcac gagaataagc gaccgtcggg
     2221 aactgcgact ggccgccatg tttttgtatc tcaaccggca ctgctttaat ggcctgtacc
     2281 gggtgaacgg aaaaggtgac tttaacgttc ctttcggtgg ctaccggact ccctacttcc
     2341 ctgaacggga aatccgcgca tttgcagaca aggcaaacag cacccgaaca ctgcttgttc
     2401 atggcgattt tcgaaaaacg ctgagctgtg ccagccacct gttcagcatg ggtaacacgc
     2461 tttgcgtgta ctgcgatccc ccttacctgc cttgcaatgg ccgggacaat ttcaccgcct
     2521 acggaaaacc attcaatgaa ggcgaacaca ttcgcctgag agcaacgctt gaccatcttt
     2581 cacaggaaac ggacggcatg acctgcatca ccatatccaa ttccgacaca ccggaaacca
     2641 gacgtattta tcagggttac cggatgaact gcatacaggc accacgaagc gttggcgcaa
     2701 gaacccgcga accagcaccg gaggttatcg ccaccctgaa acagtgcgac gcctgcggtc
     2761 gccatggcgg tggctactgc ccggactgcg gaccggtaac gggagatgca acttactgcg
     2821 tgatgtttac ccctgccagt acgtgaataa acagatgaat cagtaacaca ggagtcagga
     2881 aatgaatagc gaaagaaaca aagcactttt gcgggggtta cgtcacatcg cccgtgctca
     2941 cgaggaaaca gccaaagttg cagctgccgt tggaaaacca aaggtcgtta tatcttcctc
     3001 gatctcactg gggatcacca tggaagcaat ccgggtgatt caggatcagg aaagagaaat
     3061 cgccgccctt aaaaagctac tccacggtaa atctgccggg caaggagtca agcaatgatc
     3121 aaggagtcaa ccgttatcag attcacaacc cacggccatc agtacgaagt cgacgaaagc
     3181 ctgattgatc agggcatgac acgccgggat tcacgcaatc ccgatatata tcacatccgc
     3241 cttatcaacg gttcgcactt ccgcgccacg aacgtggaag aagtgcgcgt gcttaccaca
     3301 agctgccgtc gtgctctgtg aactgttacc gggatgagtg atcatcatgc acacacaaaa
     3361 aaaccgcttg ccagcgttcg tctggtcggg ttacagtttt cccgcacctc ataaaacggg
     3421 tgccgggttt cgcagcctgc cgactacaag agcgcacaac cgcgccagcg gtttttttgt
     3481 gcgtactgta tcgccacgtc tttttcgcgt cagaattatg gcggggcgta cggggccgac
     3541 ttcggtcggg ccgggatctc ttgtagccgg tactgcgaac ctcgtacgtc tcgccaccca
     3601 cagtttcgca gctctggatg gtgagttttc agaacttact acaagaggcc aaacaccatg
     3661 gcaaaccgca gacaacagcg cgcccgcgct gagcgtcttc acacccggac tgaaatcaac
     3721 cgcagactct ggcgcgcatc acgcgtcgcg caaatcatgt acatcaacat gctgcatgaa
     3781 cacgcacacg cgctgtcaga ccgttactgt gccgccgtct tcagctatct ggcggatgac
     3841 ctggcagaac tgcaggacct tatcaaccag caacaccacc actaaacaac atcctgaagc
     3901 catcccgcac ccgtgcgcgg gaggcttccg cacgtctgtt accggaggtt ctcatgaaga
     3961 gcgttaaggg actttcagat attcaggact tttttaactg gatgtgggca gtggatcccc
     4021 ggctggcttc ccgcttcaac gactggcacg actattaccg ccgcgcctgt aaacaggccg
     4081 ccagacgcgt caccacatac ggggatttct ccttcaccgt tgacgaccgt taccgcgtgg
     4141 acgtgaaagg cgatggcgat ggctttggtt tatttcacat caacggaacc gacagcaccg
     4201 ccatcagctg gtcgtcgtca gagatgacgc ttgttctgga aatgacaacg cacagcgtgg
     4261 ccgtgaaagg catatacacc gcagaagact ttatcggtga ataccagcgc cttctcgtgc
     4321 tgtaccgcgc ggcgtttcag gcggcgactg ccggggagtg tgacgcatga gcgaccataa
     4381 cgcacagctg gcgtacccgt ggaacgcccc gctaccggtt atcgatcctg aaacgttcgg
     4441 cagagcgcag ccaaccgcat tacgccaggc cattcagagc tacatcaacg aggatattcg
     4501 cactgacgc
//
LOCUS       DAZKGF010000265         4497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZKGF010000265 DAZKGF010000000
VERSION     DAZKGF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.265"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(18..338)
                     /gene="chbB"
                     /locus_tag="ACHOQV_004792"
     CDS             complement(18..338)
                     /gene="chbB"
                     /locus_tag="ACHOQV_004792"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFP9633036.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(637..975)
                     /gene="osmE"
                     /locus_tag="ACHOQV_004793"
     CDS             complement(637..975)
                     /gene="osmE"
                     /locus_tag="ACHOQV_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFP9633037.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1177..2004
                     /gene="nadE"
                     /locus_tag="ACHOQV_004794"
     CDS             1177..2004
                     /gene="nadE"
                     /locus_tag="ACHOQV_004794"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP9633038.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2234..3121
                     /gene="cho"
                     /locus_tag="ACHOQV_004795"
     CDS             2234..3121
                     /gene="cho"
                     /locus_tag="ACHOQV_004795"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFP9633039.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCW
                     PWKGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATMLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3081..3656)
                     /gene="ves"
                     /locus_tag="ACHOQV_004796"
     CDS             complement(3081..3656)
                     /gene="ves"
                     /locus_tag="ACHOQV_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFP9633040.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3859..4344)
                     /gene="spy"
                     /locus_tag="ACHOQV_004797"
     CDS             complement(3859..4344)
                     /gene="spy"
                     /locus_tag="ACHOQV_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFP9633041.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTSAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1118 a         1097 c         1071 g         1211 t
ORIGIN      
        1 ggaaaattta aaataaatta atttgctgcg gcttttttaa tcgctgcaac cgcagcctta
       61 agcacgccta aaccatcgac tttgccataa agcagcgagt caattacttc gaccggtttg
      121 ttgggtaaca aacgctggat ttcgggcaac atataagcaa tctgcggccc taataacacg
      181 acatcggcat tctgaccttt ttcaccagcc agtgtttccg gaaatgcttc aataatgacc
      241 ggaacttcat atttttctgc ctgtgcgcgc atttttgata ccagtaaaga ggtagacatg
      301 cccgcagaac aaaacagata aatgtgtttc ttttccataa aactgccctc atcgacgatt
      361 atctgtcagc cagacactcc gcaagcctta acctgcttcc atgctctggg taacttgcga
      421 aaccaacatg actttttttg tggctgaaag gagtatacgg tatagacctg agcaatagta
      481 tttctttgac ctgataaatt gtctctcctt gacctgcggt tatgaccctc ttcacatttc
      541 gggcaaataa ttcgcgaaga taattaagaa ctcaggccaa aaaaaaccgg cgcaatggcc
      601 ggtttccgtt gtaactcaag ctttcaggcg aagtgattac ttcgcagcct gtggatcagt
      661 gtcgtattca gcacaggtct gataaccgga gttgatgaca tgtccggtat catctaacgc
      721 gacaaagtag gtttctgctt taccatcacg ttgacccagg atgtaggtct ggcaagtacc
      781 acgagcatgg atcatgctca cttcagacga aggtttaccc gcaatttgtg caacctgcgc
      841 ccggctcatg ccttttttga cgtctttcac cacaggctgt acaaactggt ctttggtacg
      901 atcataagcc gtacaacccg ccagcatggt taataccgcc gctgcactca gaattcctgc
      961 catattcttg ttcatattcc gtcctcttgt ttatcagcgt gttagataag cctggaatac
     1021 attgggcgct ttttcaagcc cgtgaacgaa acggctccgc tttcagagga ttcctgtatg
     1081 acgttttaac caccattcag cccgctgtcg cttgtcgttt cagtagcaac gggttagctt
     1141 taaggaagtt ttgtcttttc tgtctggagg ggttcaatga cattgcaaca acaaataata
     1201 aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg aaattcgtcg tagtgtcgat
     1261 tttctgaaaa gctacctgca aacttatccg ttcattaaat cactggtgct cgggatcagc
     1321 ggcggtcagg actccacgct tgccggaaag ctgtgccaga tggcgattaa tgagctgcgc
     1381 caggaaactg gcaacgaatc actgcaattt attgccgtac gcctgcccta tggtgttcag
     1441 gctgacgaac aagattgcca ggatgccatt gcctttattc aaccggatcg cgtattaacc
     1501 gttaatatca agggcgcggt attggccagc gaacaggcgc tgcgcgaagc gggtattgaa
     1561 ttaagtgatt ttgtccgtgg caatgaaaaa gcgcgtgagc ggatgaaagc acaatatagc
     1621 attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc atgcagcaga agccattacc
     1681 ggattcttca ctaaatatgg tgacggcggt acggacatta atccactgta tcgtcttaac
     1741 aaacgtcagg gtaaacagtt actggcggca ttaggttgcc cggaacacct ttataagaaa
     1801 gcgccaacgg ccgatctgga agatgatcgc ccttctctac cagatgaagt ggcactcggc
     1861 gtgacctatg acaatatcga cgactatctg gaagggaaaa acgtacctga acaggtcgcc
     1921 agaacaatag agaactggta tctgaaaact gaacataaac gccgtccgcc aattaccgtt
     1981 ttcgatgatt tctggaagaa gtaataattt gcacatattg gtttgtgcga aaaagagtac
     2041 tttgttcacg ccggatgcgg cgtgaacgcc ttattcgccc cacaaaacta tgcaaattca
     2101 atatattgca tgagtcgaat aggcctgaca ggcgtagcac gtctgacggt gtaacctttg
     2161 tcatcaaccc gcctcttttt taatcgctcc ccgcctgtta cactggatag ataaccagca
     2221 ttcggagtca actgtggtac ggcgtttaac ttctccgcgg ctcgaatttg aagctgcggc
     2281 aatttatgaa tatcccgaac atttacgttc attccttaat gacttaccca cccgaccagg
     2341 ggtgtatctg tttcatggcg aaagtgacac catgccgctc tatatcggca aaagcattaa
     2401 cattcgcagc cgcgtccttt ctcatttacg taccccggat gaagccgcca tgctacggca
     2461 atcccgacgc atcagctgga tatgtaccgc cggtgaaatc ggcgctctac tccttgaagc
     2521 gcgattaatc aaagagcagc agccgctgtt taataaacgc ttgcgccgca atcgccagct
     2581 ctgtgccctg caattaaatg aaaagcgcgt cgatgtggtg tatgccaaag aggtggattt
     2641 ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg cgcgcagctt tgcaagcatt
     2701 gcagaccatc gctgatgaac aaaaactttg ttatggtctg ttgggactgg agccgttaag
     2761 tcgcggtcgt gcatgttttc gttcagcgct aaaacgttgc gccggagcat gctgcggtaa
     2821 agagagccat gacgatcatg cgctacgttt gcgccagtct ctggaacgtt tacgagtagt
     2881 ttgttggccg tggaaaggtg cggtggcgct gaaagaacag cacccggaaa tgactcaata
     2941 tcatattatt caaaactggc tgtggctggg ggcggttaat tcgctggaag aagcgacaat
     3001 gttaattcgg acacccgccg ggtttgatca cgacggttat aaaattcttt gtaagccgct
     3061 gctttccggt aactatgaaa ttactgaact tgatccggcg aatgaccagc gagccagttg
     3121 atttcactga acaacagttt accttgcggt tgtaaaagcc gcagggtgtg cctgccatcg
     3181 aaccagcacg cgccctggtc ggtagtgagc agtttatcgc ccaattgcca cgccccgtta
     3241 atgacaaaca ctaccccgcc acgtgagcca aaggtagtaa acgtacgttc ggcaattctg
     3301 actttcgcct tacagacatc caggcgtgtc atgatgttga aatccatcga catctgccct
     3361 gccgtcagtt tcgcttttac cacctggtcc gccgcaaagg caaaaggctg taacggtttt
     3421 aaggtatggt taaagcggtc tgtgctttca aggaacatct cgccgccttc cagcaacgtc
     3481 actatccttt ccatacctgg aaacaaagaa aactccccat tcgccgcgat ggatgcaatg
     3541 ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag tgcaaatttc gcgcgtttca
     3601 ccggcagcat ttcgccacag attcaccgac attttacgca tatcaaagta ttccatactc
     3661 gctcccggcc cgttcttttc attgttatcg ttcaggtaac cgtggatggt tatcggcaac
     3721 tcgggtgatt tacgttagtg gcgatcaggc tttttttgca tctgtttgag aacaaaaaag
     3781 aaaaccgccg atcctgtcca ccgcattact gcaaggtagt ggacaagacc ggcggtctta
     3841 cgttttttgg ctgaaagatt attcagcagt tgcaggcatt ttaccttttg ccgctggacg
     3901 ttctgtcaga cgcttctcaa aattagcatt aaattgcttt ttctgttccg gcgtcaagat
     3961 gttgtaaatc ttgttctggg tttccatgtg cgccagcatg ttagctttgc gctgttcttc
     4021 catttttgcg atctgcgctt cagcttttgc tttatcgaag gtatcgctgg caatgatgtc
     4081 atgcattgcg cggcgttctt ccagcggcgg acgtttcatc tggtcacgct ggcctttcat
     4141 gatttcgcgg atctgctgtt tctgcgcgtc ggtcaggttc aggtctttga acatcatgtc
     4201 ctgatgcgga ccgaacttgc ctttgtggtg catcatcggc ttcgcgtcag ccggtgctga
     4261 ggtagtggtg tctgcggcat gggccaggtt agccgcgcca agagccaggg tagaggcaac
     4321 aaacagtgca gttaatttac gcatattcta tatccttcct ttcagttatt tattacggct
     4381 ttcttaagta gcgtgccgtg ttgacgagat taactttact gactttagcg tcaattaatc
     4441 agagcaacgg taaaacaatg aaagtgtaaa aaacactttt gcgccaatta tggagaa
//
LOCUS       DAZKGF010000266         4462 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZKGF010000266 DAZKGF010000000
VERSION     DAZKGF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.266"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            25..548
                     /locus_tag="ACHOQV_004798"
                     /pseudo
     CDS             25..548
                     /locus_tag="ACHOQV_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
     gene            789..1754
                     /gene="lipA"
                     /locus_tag="ACHOQV_004799"
     CDS             789..1754
                     /gene="lipA"
                     /locus_tag="ACHOQV_004799"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFP9633042.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1855..2058)
                     /gene="tatE"
                     /locus_tag="ACHOQV_004800"
     CDS             complement(1855..2058)
                     /gene="tatE"
                     /locus_tag="ACHOQV_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFP9633043.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2187..2975)
                     /locus_tag="ACHOQV_004801"
     CDS             complement(2187..2975)
                     /locus_tag="ACHOQV_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFP9633044.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLDRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARVLDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFALPQLL"
     gene            3068..3451
                     /gene="crcB"
                     /locus_tag="ACHOQV_004802"
     CDS             3068..3451
                     /gene="crcB"
                     /locus_tag="ACHOQV_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFP9633045.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3505..3714)
                     /gene="cspE"
                     /locus_tag="ACHOQV_004803"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3505..3714)
                     /gene="cspE"
                     /locus_tag="ACHOQV_004803"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP9633046.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3889..4449)
                     /gene="pagP"
                     /locus_tag="ACHOQV_004804"
     CDS             complement(3889..4449)
                     /gene="pagP"
                     /locus_tag="ACHOQV_004804"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFP9633047.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYDP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1178 a         1138 c         1003 g         1143 t
ORIGIN      
        1 catgaataaa attggagtaa gtgcgtggat agtaataatc aaattgaacc ctgtttaagc
       61 agaaaatcgt cagaaggtaa accacaaata tttacaacct tacgaaatat tgatcttacc
      121 cttctgacta ttttgaagtt gcatatgtcc ataaaggaat cgttaatgca gcgaaagtgc
      181 ttaatctgcc cccctcggca atctatcagc ctattcagaa actgcgcgtt atattccctg
      241 acctattgtt tattcgcaaa ggccagggtg tcactcctac cgcatttgcg atgcatctat
      301 atgagtctat cactcaggga cttgagtcca ttcttggcgc gctggatatc gaaggaagct
      361 atgataagca acggacgata actattgcgc cctccccctc ggtcggggcc caggtccttc
      421 ctgtcatcta tcgggcgatt aaaactcact atccacagct tttactgcgc aacccgccca
      481 tcggcggcgc ggaaaaccaa ctctgtcagt ttcaaaccga tctcatcacc gataacatgt
      541 tttgcacagg accatagatg ttatccgtaa tgcattttga aaaagtattg ttacagccag
      601 atcacagcgc agaacgataa aatggcacaa tccaacaaca attttacatt ttcgcgaccg
      661 ctttggctgc tttcaggtcc gtttcaatga tatactgccg gtcgttaatt caaaaatagt
      721 tgataattac aacaatctat tgaattgaaa cgctttcctt cgtaattcgc aactggaaca
      781 cgcacgctat gagtaaaccc attgtgatgg aacgcggtgt taaataccgc gatgccgata
      841 agatggccct tatcccggtt aaaaacgtgg caacagagcg cgaagccctg ctgcgcaagc
      901 cggaatggat gaaaatcaag cttccagcgg actctacacg tatccagggc atcaaagccg
      961 caatgcgcaa aaatggcctg cattctgtct gcgaggaagc ctcctgccct aacctggcgg
     1021 aatgcttcaa ccacggcaca gcaacgttta tgatcctcgg cgctatttgt acccgccgtt
     1081 gtccgttctg tgacgttgcc cacggtcgcc cggtagctcc ggatgccaat gaaccagtga
     1141 aactggcgca aaccattgcc gatatggcgc tgcgttatgt ggttatcacc tccgttgacc
     1201 gtgatgacct gcgcgatggc ggtgcccagc actttgcgga ttgcattact gccattcggg
     1261 aaaaaagccc gcaaatcaaa attgaaactc tggtgccgga tttccgcggt cgtatggatc
     1321 gtgctctgga tattctgacc gccacaccac cagatgtgtt caaccataac ctggaaaacg
     1381 taccgcgtat ttaccgtcag gtacggcctg gtgcagatta caactggtcg ctgaagctgc
     1441 tggaacgctt taaagaagcg catccggaaa tcccgaccaa gtctggtctg atggtgggtc
     1501 tgggtgaaac caatgaagaa attattgagg taatgcgcga cctgcgccgt catggtgtga
     1561 cgatgctaac gctggggcaa tatttgcagc caagccgcca tcacctgccg gttcaacgtt
     1621 acgttagccc ggatgagttc gacgaaatga aagccgaagc gctggcgatg ggctttaccc
     1681 atgctgcatg cggtccgttt gtccgctcgt cttaccacgc cgatttgcag gcgaaaggga
     1741 tggaagttaa gtaatcctgt aataattact tacatctgtc tgataaaaaa cccgcttttt
     1801 gaagcgggtt ttttgtatcg aacaagatat tgagggagcg tcctgctcgc cacgtcactc
     1861 tttatgagag agcttttcag cctgaagatc aacgtctgcg ccttttttcg ccgcagcatc
     1921 gtcatcattc atcgccttct tgaacccttt aatggccgct ccaaggtctc cgcccagcgt
     1981 acgtaacttc ttagtcccaa acagcagaac gaccagcgcc gcaactacca gcagtttggt
     2041 aatactaatc tcacccatag ataccttctt gacatataac cggcaacaga acgcgcctta
     2101 aaaaccagag aatattaacg gtcattcgac gcgcgcacac aatagcaatc tgtaacaagg
     2161 tgaatcaagc gccgagtaaa aaaacatcat aataattgcg gcagcgcaaa gcgacggttg
     2221 tttaagacgg gcagttgcgc gcgtacctga cgcacacgtt cgggcgtcac ttccgccata
     2281 atgagtgcag gcatttctga cgctgccgca atggtgacgc caaagggatc tataatccgg
     2341 ctttgcccga tatttttgtt cccgcactcc cccgccgcca ccatataaca ggtggtatcc
     2401 agcacacgag cggcaagcaa cgttgaccag tgatgctctt tgagcggccc acgaacccag
     2461 gcggcaggaa gtaccaggat ttcagctccc tgtaatgcct gtgccagcgc cagctctgga
     2521 aagcgtaagt cataacaggt catcagaccg accttcatcc cttccacctc cagtaacgga
     2581 gctatttcat taccagcatc aacatggcgt gattcctgaa tggcaaatgc atcatagaga
     2641 tgcagtttgg cataacgggc gacgatgttt cctgcctgta gcgccaccag catattccac
     2701 gcgcgccccg gtgttgaagg aacatgaatc gtcagaattg tcgtcatcat gttacgttta
     2761 ctttctcgcc gtaaacggtc gaggaattcg ccttccagca gctgtgctga tttaaccgaa
     2821 agatccgcat catgatcatc acgcgccagt aatgcttccg gcaggacaaa cagcgaaacc
     2881 tcgttttccg ccgcctgcgc catcaacgag gcacaaatct cagcgttctt ttcccacaca
     2941 gatgtaacag caaactgtcc tgcggcaacc agcattgcca cctcctgtca gagtcgtaaa
     3001 attcgccaac ggcgattatt cggcgttaca cttatcactc atacaaatca aatagcagga
     3061 ttttgcagtg ttacaacttc ttttagcagt ttttattggc ggtggtacgg gaagcgtggc
     3121 gagatggctg ttaagtatgc gatttaaccc gctgcatcag gcgattccgt tggggacgct
     3181 ggctgcaaac ctgattgggg cattcatcat aggaatggga ttcgcctggt tcagcaggat
     3241 gacgaacatt gatccagtgt ggaaagtatt aatcaccacc ggattttgtg gcggtctaac
     3301 aaccttctca acattttcgg cagaagtggt gtttttgtta caagagggcc gctttggctg
     3361 ggcgttactg aacgttttcg tcaacctttt agggtctttt gccatgaccg cactggcttt
     3421 ctggctgttt tccgcctcaa ccgcacacta aagaccaaaa aaaacccgct gattaagcgg
     3481 gttttgaatt cttgctgacg tatcttacag agcgattacg tttgcagcag aagggccttt
     3541 ggcaccgtta gtgatttcga actctacgcg ctgaccttca gcaagagttt taaaaccatt
     3601 agtctggatt gcagagaagt gtacgaacac gtctttgctg ccgtcttccg gagtaatgaa
     3661 accgaatcct ttggactcat taaaccactt aacgttacct ttaatcttag acatcaaaat
     3721 tacctttaca tgaaaaatag acacaaatgc tgtgtcggtt accagtacac caattgtggt
     3781 acgctttgtc cagtgcaact ttactaaaaa gtgataaaag tcgcgttatt tttgatgtta
     3841 attattttac ccattgattt taaagagttt actaaaattt catttgtctc aaaactgaaa
     3901 gcgcatccag gcaaagtaca cattgccatt gttataggta cccggaatgt aggtcatctg
     3961 aaaagtcact ggatcataac ccacagaggc caatggcagt agaaccggga gagggatgta
     4021 attccagtta tcgcgtgccg ttacgccagc ggtgaatccc agtcctaaat gaaaattttc
     4081 atccgccagc ggtcgccagg tactttccca tccgtatccg gcaatcggtt cccatttgtt
     4141 ccacgagtcc ttaaatgcca tggcatacag gccatgccag tttccttttt catcccaacg
     4201 cgactgacca aaaccgccac cccacggtcg ctcgttatag cgatcggttt tttctttgtc
     4261 gtaagcgaaa cgtgcatgcc aggtgatggc aggaatatat aaatcataat gttcaggctg
     4321 ttgccaggtt tgtgcaatat tttctctaaa cgttgtcatc cactcatctg cgttagcaaa
     4381 aactttacca acgctgatta actgaataaa aacaaaggaa aagatagcga catatttact
     4441 cacgttcatt tgtgaccata aa
//
LOCUS       DAZKGF010000267         4446 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZKGF010000267 DAZKGF010000000
VERSION     DAZKGF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.267"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..260
                     /locus_tag="ACHOQV_004805"
     CDS             <1..260
                     /locus_tag="ACHOQV_004805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633048.1"
                     /translation="IYHVDVKKLDTYLVEKKKADAFDDFMWGKKSKNESENLTFDDCY
                     KVFNFEAKAISSSVLEGKGISVRMDGDRLIFDGKRFVRKKS"
     gene            286..1233
                     /locus_tag="ACHOQV_004806"
     CDS             286..1233
                     /locus_tag="ACHOQV_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFP9633049.1"
                     /translation="MQVNVAETLFRTIDTATQSQMANMNTVMSVMGIVIGGIWMTYIL
                     MKSLYWYFEGLTTVIQDAFFTIFKAFAIMMASLTISWYTSTVIPPVLEFPLWLANTIA
                     GVNNDSSNLVDMVINSYISGLENLVDKMKFGFSVSSIKSMVTVGVAVVLYLLSGLPFL
                     GVAIATLLTLKVATNLILIIGPMFIACLAFPKVAHWFWGWVGVLGGFVLTQALFAITL
                     GMAISFINSNMINNGDFNISMVDIICMSLYFSAFTLLATEIPNYAASIMGGAPSGGVG
                     SMSRLLGKATGFGAASHMSKALGSKLVDRFHGRGKGRIS"
     gene            1450..2313
                     /locus_tag="ACHOQV_004807"
     CDS             1450..2313
                     /locus_tag="ACHOQV_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFP9633050.1"
                     /translation="MLFKSNKIKKETEQERLLHYGDGKLSGKSDEIGREIRAYTMAAR
                     WFEKRVAEDYRKKARNSRILAGLFGVIAIVAVIAVANLAPLKTVEPFVIRVDRNSGYM
                     DVVRPGWSKEDTKDISDDKHFISLYILSRETYNWASQKSNFAIVRQLSYSDVFSEYRN
                     FQLSGKGYVSTLNQSRQISVDIDSIVPLPVSTDKKLGERDDIKTYQVRFRQSLLDAEG
                     RPVPETGQILKLDENGKPIVKPKVVFWTAIISFDYLNTADTEGEAWLNPKGFGVLAYT
                     KTEEIRNEENN"
     gene            2297..3109
                     /locus_tag="ACHOQV_004808"
     CDS             2297..3109
                     /locus_tag="ACHOQV_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFP9633051.1"
                     /translation="MRKIIKYIVPVCLIFSVTGFVHAEIAGKGSVSDKRIQTALYSPD
                     NVFRIYAMKDRTTMIQLEQGETINTDAGAMNLGKPGTEGAPEWLLGANKAGSLIMIKP
                     SRYAEDPRTNLIVSTNRRTYLFELHLTQSPALATYLFRFNYPKPPEVGKNPFVGRNIN
                     ENPCDGIINKKYQKRGDMIISPYQVWDNGTFTCFRFPTNAPRPVIYEVLPDGTETMVN
                     ARPVNDILVVHGVSREFRIRLNRLVLAVRSNVENTGWYNYKGTTTGEVREVK"
     gene            3109..>4446
                     /locus_tag="ACHOQV_004809"
     CDS             3109..>4446
                     /locus_tag="ACHOQV_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFP9633052.1"
                     /translation="MTENVVTPPENETKKETSAADENKPQGQGVGAFSLKPAGRKKPV
                     NWKIVGVIIGGIVIVLLFTIIFAAIVINNRADPEPEIDETGIKAEVVTRSIGVIDDPM
                     GAFMEAHLPQDRDKTESDSRTVQSSGKQESNGPVSDKKNTQTSGGQSTVNPVTPVTDI
                     RVPETLFSPVARFDSSGISQGVGGSSDGVTSSSGYDVSAEEARLREFANADPAEIVNR
                     ALQSQADTVNGGISSDMRSNILGDLSSSRQYATAKAYKSPPRKYLLKRRTNFQCVLYT
                     AVKTDHSGFVGCRLTKPIFSADGSVILAEAGAELNGEQKIEVKQGQSTVFTSWSELET
                     TAGTEGGIRADLNGLGTGPMGSSGTDAYIDNHYGQRFGGAVMLSFIQDAIATAANAAQ
                     KNKSTYSFDNSEDNAESMAEKALENSINIPPTGYVLPGTVINVIVAQDIDFSSV"
BASE COUNT         1399 a          673 c         1037 g         1337 t
ORIGIN      
        1 gaatttatca tgtggatgtt aaaaaactag atacttatct ggtagaaaag aaaaaagctg
       61 atgcatttga tgatttcatg tgggggaaaa aaagtaagaa tgaatctgaa aatttaacat
      121 ttgatgattg ttataaagtg ttcaattttg aagctaaagc aataagtagt tcggtattag
      181 aaggtaaggg tatatctgta aggatggatg gtgatagatt aatttttgat ggaaaaagat
      241 ttgtaagaaa aaaatcataa tgtgacatag ctaaaggatt tttatatgca ggtaaatgtc
      301 gcggaaacgc tgtttagaac aattgatacc gcgacacaat ctcagatggc aaatatgaat
      361 actgtcatgt ctgttatggg gattgtgata ggcggtatat ggatgacata tatattaatg
      421 aagtcattat attggtattt tgagggctta acaacagtaa tacaggacgc attttttaca
      481 atatttaaag catttgcaat catgatggca tcattgacta tatcatggta tacatcaaca
      541 gttattcctc cagttttaga attcccttta tggttggcta atacaattgc aggagttaac
      601 aatgattcgt caaatttagt tgatatggta ataaattcat atatatcagg actggaaaat
      661 ctggttgata aaatgaaatt tggattttct gtatcatcaa taaaaagtat ggttactgtc
      721 ggtgttgctg ttgttttata tttactaagt ggattgcctt ttttaggggt ggctatagca
      781 acattgctga cgcttaaagt cgcaacaaat ctaattctta tcattggtcc aatgtttatt
      841 gcatgtttag cttttcctaa agtagctcat tggttttggg gatgggtggg agttttagga
      901 ggatttgttc ttacacaggc cctttttgct attactcttg gaatggctat ttcatttatt
      961 aatagcaata tgattaataa tggggacttt aatatatcaa tggttgatat tatatgtatg
     1021 tctttatatt ttagtgcatt tactttactg gctacagaaa ttccaaatta tgctgcatct
     1081 attatgggag gcgctccatc tggtggggta gggagtatgt ccaggctttt aggaaaagca
     1141 actggttttg gtgctgcaag tcacatgagt aaggcattag gaagtaaatt agttgatcgt
     1201 tttcatggta gagggaaagg tcgtatttcc tagtaatttt tctatctctg cttataatcc
     1261 attttctcgt cttctttaca aaaacttcat ttataaaaag gcttatcatg aaaaatatat
     1321 tcgctatatg tgcggcgctt tgtgttgtgc tgggatgcat atcatgtagt tcaccgccag
     1381 agttgtccca accatctggt gaatggattg atgtaaatcc agagtctgtg ctgttaccgg
     1441 gagaaaaaaa tgttatttaa aagtaataag ataaaaaagg aaactgaaca ggaacgtctc
     1501 ttgcattatg gtgacggcaa attatcaggt aagagtgatg aaattgggag ggaaataagg
     1561 gcttacacaa tggctgcccg ttggtttgaa aaaagggtgg ctgaagatta cagaaagaaa
     1621 gctagaaata gtcgtatttt ggctggattg tttggtgtta tagctattgt tgctgtaatt
     1681 gcagttgcaa atttagctcc acttaaaaca gtagagcctt ttgttatccg agtagatcgc
     1741 aatagtgggt atatggatgt tgttcgtccc ggatggagta aggaagatac aaaagatatt
     1801 tctgatgata agcattttat atcattatat attttatcta gagaaacgta taactgggct
     1861 agtcaaaaat ctaattttgc aatagtaagg cagttgtctt attccgatgt gttttctgaa
     1921 tatcgtaatt ttcagttgtc aggtaaaggg tacgtctcaa cattaaatca gtctcgtcag
     1981 atttctgttg atattgattc aatagttcct ttacctgtca gtactgataa aaaattaggt
     2041 gagagggatg acatcaaaac ttatcaggtt cgtttcagac aatcactgct tgatgcagaa
     2101 gggcggcctg ttccagagac aggacagata ctgaagttgg atgaaaacgg gaaaccaata
     2161 gttaagccca aagtggtttt ctggacagca ataatatctt ttgattattt aaatacggct
     2221 gatacggaag gtgaagcctg gttaaatcct aagggctttg gtgtgcttgc atatacaaaa
     2281 acagaggaga tcaggaatga ggaaaataat taaatatata gttcctgtat gtcttatatt
     2341 ttcagttact ggttttgttc atgcggaaat agccggaaaa ggttcagtca gcgacaaaag
     2401 gatccagacg gcattgtata gtcctgataa cgttttcaga atttacgcca tgaaggacag
     2461 aacaacaatg atacaactgg aacaagggga gacaataaat actgatgcgg gtgcaatgaa
     2521 tctggggaaa ccaggaacag agggggcacc tgaatggtta ctcggtgcta ataaggccgg
     2581 aagtttaata atgataaaac caagtcgata tgcggaggat cccagaacaa acttaattgt
     2641 aagtacgaat cgccggacat atttatttga attacatctg acacaatcac cggctttagc
     2701 aacatatctg ttcaggttca attatccaaa accgccagaa gttggtaaaa atccatttgt
     2761 tgggaggaat ataaacgaaa acccgtgtga tggtattatt aataaaaaat accaaaaacg
     2821 tggtgatatg attatttcgc cttatcaggt atgggataat ggtacattta cctgttttcg
     2881 ttttccgaca aatgcgccac gtccagttat ttatgaagtt ctacctgatg gtacagaaac
     2941 tatggttaat gcgcgtcctg taaacgatat tttggttgtg catggtgtta gtcgtgagtt
     3001 cagaattcgt cttaacagac tggttcttgc tgtacgttct aacgtagaaa atacaggatg
     3061 gtacaattat aagggaacaa ctacaggtga agtcagggag gtcaaataat gactgaaaat
     3121 gtagtgacac caccagagaa tgaaacgaaa aaggaaacta gcgctgctga tgaaaataag
     3181 cctcaaggtc aaggtgtggg cgcattttct ttaaaaccag ctggtcgtaa gaagcctgtt
     3241 aactggaaaa tagttggtgt aattattggc ggtattgtta ttgttctttt gtttacaatt
     3301 atatttgccg caatagttat taataatcgt gcagatccag aaccagaaat agacgaaaca
     3361 gggattaaag cggaggtcgt aactcgctct ataggggtaa ttgatgatcc aatgggagcg
     3421 tttatggagg ctcatttacc acaagacaga gacaaaacgg aatctgacag tagaaccgtc
     3481 cagtcgtcag gaaagcagga gagtaacgga cctgtttcag acaaaaaaaa tactcaaact
     3541 tcagggggcc agtctacggt caacccggtt acaccggtta cggatatcag agtaccagaa
     3601 acgttgtttt cgcctgttgc acgatttgat tcttccggca ttagccaagg agttggtggt
     3661 agtagtgacg gtgtaacatc atcttctggt tatgatgttt cggcagaaga ggctcgtcta
     3721 cgagagtttg ccaatgcaga tccggcagag atagttaacc gtgctttaca gagtcaggca
     3781 gatactgtga atgggggaat atcttcggat atgagatcta acatcctggg ggatctctca
     3841 tcatcccgcc agtatgcaac agctaaagct tataaatcgc ctccacgtaa gtatctcctg
     3901 aaaaggagaa caaattttca gtgcgtactt tatacggcgg tgaaaacgga tcactctgga
     3961 ttcgttggtt gtcggttaac aaaacccatt ttttcagcgg acggtagtgt tattttggct
     4021 gaagcaggtg ctgaattgaa tggggaacaa aaaattgaag taaaacaagg gcaatctacg
     4081 gtgttcactt catggagtga gcttgaaact actgccggga cagaaggggg tatacgagca
     4141 gatttaaatg gattagggac ggggccgatg ggaagcagtg gcacggatgc atacattgat
     4201 aatcattatg ggcagaggtt tggtggagcc gtgatgctat cgtttattca ggacgcgata
     4261 gcaacagctg caaacgcggc acaaaaaaat aaatcaacgt atagttttga taacagtgag
     4321 gataatgcgg aaagtatggc tgaaaaagca ctggaaaatt ctataaatat accacctaca
     4381 ggatatgtat tacctggaac agttattaat gtaatagttg ctcaggatat tgatttttcg
     4441 tcagtc
//
LOCUS       DAZKGF010000268         4404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZKGF010000268 DAZKGF010000000
VERSION     DAZKGF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.268"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..419
                     /locus_tag="ACHOQV_004810"
     CDS             <1..419
                     /locus_tag="ACHOQV_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633053.1"
                     /translation="IQNLSTGRPVLLGDNLHELNEFIENTRQQFTPNTINWWRTPFIA
                     LNNWINQETVNTIGGYIQMVTAHPLGTRISFLTNLNENIIRTSYAGINTTESFGPTIG
                     GFILMPMDGMSLPDENGWDVGNQVTRTAADRVATNR"
     gene            complement(422..646)
                     /locus_tag="ACHOQV_004811"
     CDS             complement(422..646)
                     /locus_tag="ACHOQV_004811"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9633054.1"
                     /translation="MKNTSSIQAQFGAHLKKLRLQSGLSQEAFADKCGLDRTYVSGIE
                     RGVRNPTLEVISVLGKGLDMDIKELFNFER"
     gene            complement(686..949)
                     /locus_tag="ACHOQV_004812"
     CDS             complement(686..949)
                     /locus_tag="ACHOQV_004812"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9633055.1"
                     /translation="MEKTGSIKKVLGKRIRALRREMDLSQEALADKCGIDRTYMSGIE
                     RGVRNPTLVVICAISDGLGMDLSHLFMFDDGAEKDAESTKCDS"
     gene            1057..1332
                     /locus_tag="ACHOQV_004813"
     CDS             1057..1332
                     /locus_tag="ACHOQV_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9633056.1"
                     /translation="MTKKDWHPADIIAALHKRGTSLAKLSREAGLASSTLANALNRPW
                     PKGEWLIAIALGKHPSEIWPERYLNTGNKREPRKPLHLMTTTENSGT"
     gene            1551..1925
                     /locus_tag="ACHOQV_004814"
     CDS             1551..1925
                     /locus_tag="ACHOQV_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9633057.1"
                     /translation="MMTYCARIVAIGLFVADGLADRTLITFDSNGPKDCLDYSLSLEP
                     SFREDDLLILPGDRLLLASHDYLVTAVGKEAQQSLFELGHLTMVFNGSLNACHSGAVH
                     LSGPIPKLHDLYGELLIEEGRP"
     gene            1969..3327
                     /locus_tag="ACHOQV_004815"
     CDS             1969..3327
                     /locus_tag="ACHOQV_004815"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9633058.1"
                     /translation="MELNRSLKARHLTMISVGGVIGAGFFLGAGAAIARTGPSVVLAY
                     LIGGVITLFIMLLLAEMAVARPVSGSFESYARTAFGPWCGFITGWTYWLAFLIGPASE
                     AIAAGTFLNLWFPGVPVWLFCLVIASSLTAINMVGVHFFGEVEFWLSLVKVVALLAFI
                     VAGCYSLGLDNASPASISHPTDSGSFFAGGLPGFLGAMLMVIFSFGGTEAIGTAAGES
                     ENPQRDIPRTLRGTVVRILLLYVGSLTVLLLVLPWQQAGVSSSPYVEASGILGGHMAR
                     HIMNFVVLTAALSCIDTGVYATSRMLHAMASDGYFPAWFARLHPAHKTPDNAILASSL
                     VLFTGVIIAILSPDAYVILAGISGFGFLFTWLMIALSQPTLHRIALHDGTLKYKAPAA
                     RYIQPLTVGLIALIMAGQFFTDGGGITLVTVLIWLALASAYYIFVVRKNRRLSDEKSH
                     AQ"
     gene            3305..4096
                     /locus_tag="ACHOQV_004816"
     CDS             3305..4096
                     /locus_tag="ACHOQV_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HFP9633059.1"
                     /translation="MKKVMLNELTGREIRELLQSGKELTAIVNFGSCENHADHLPLGP
                     DMFVPEIVACRVAQRVDNTLVLPCTPFGTSVHYNAYPLAVTLRYETNIALAEDIFISL
                     LHHGIRRILVINGHDGNIPAIEIAARNVKARHTQAAIVVVPAWWDLVGHHLGKGFFDS
                     WDNTGLGHGGEGETSVMMALIPELVELKYAQKQIPVEVFADYGVNRIWDIGEISATGA
                     TGDPRCATAEKGQRILACLVDVLCALLRKLDAANWQYALNKDANM"
     gene            complement(4199..>4404)
                     /locus_tag="ACHOQV_004817"
     CDS             complement(4199..>4404)
                     /locus_tag="ACHOQV_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633060.1"
                     /translation="TYQSRKEFLKASTEVELRGQIYLKQHSNEKLKDELANLRSKCAE
                     FVGGKVTKPELSFISAIILLIEV"
BASE COUNT          991 a         1221 c         1111 g         1081 t
ORIGIN      
        1 atattcaaaa tttatcaaca ggcagacctg ttttattagg tgataacttg catgaactta
       61 atgagtttat agagaatacc aggcaacaat ttactcctaa cacaataaat tggtggcgta
      121 ccccgtttat tgctttgaat aattggatta accaagaaac agtcaatact attggtggat
      181 acatccaaat ggtaaccgcg cacccacttg gtactcgaat atcatttttg accaatttga
      241 atgaaaatat tatcagaaca agctatgcag gaattaacac tactgagtca tttggaccaa
      301 ctattggtgg ttttatccta atgccaatgg atgggatgtc actcccagat gaaaacggct
      361 gggatgttgg aaatcaagtt acccgtactg ctgcggatag agttgctact aatagataaa
      421 cctatctttc aaagttgaat aattctttga tgtccatgtc caaccctttt cctaaaacac
      481 ttatcacctc aagggttgga ttccttactc cccgctcaat accgctcaca taagtacgat
      541 cgagaccaca cttatccgcg aatgcttcct gagacaggcc cgactggagt ctgagttttt
      601 tgagatgtgc gccaaactgc gcctgaatac tggatgtatt tttcatccag ataaaatgaa
      661 cttacgttgt ctatcagacc acggactatg agtcacactt cgttgattcg gcgtcctttt
      721 ctgcgccatc gtcaaacata aataaatggc taagatccat ccccagaccg tccgagattg
      781 cacaaatgac aacaagcgtg gggttcctta ctcctcgctc aattccactc atataggtgc
      841 gatcgatacc gcatttgtcg gctaaagctt cctgggatag gtccatctca cggcgtagag
      901 ccctgattct tttacccagt acctttttga ttgatcccgt cttttccatc tagccagcat
      961 caatcttcgt tgattatcag tccacggact atgagtcaca taaaaagcgt gctagcatac
     1021 attcgtgact catagtcaac aatagaggta aacggaatga ctaaaaaaga ctggcacccc
     1081 gccgatatca ttgccgcctt acacaagcgc ggcaccagtc tggcgaaact ctcgcgggaa
     1141 gcgggactgg cctcgtcaac gctggcgaat gccctgaacc gaccgtggcc gaaaggtgag
     1201 tggctgattg ccattgcgct gggaaagcat ccgtcagaaa tctggccgga acgttacctc
     1261 aatacgggaa ataaacgtga accccgtaaa ccgcttcacc tcatgacaac aacagaaaac
     1321 tccgggacat aacgacccgc gctgtgagtg gcaggacgca ttaatcaacg acacctgaca
     1381 ggagagttgt ttatgcacac cgaaccacac ccttgctcca tcgctcttac aaccggaggc
     1441 caccttcacc cggcctcccg gactgaaggc tttattcacc tattcaggct acatgcctgc
     1501 tcacctacac caggccgtac tctcatgccc gacaaacggg agaacgagcc atgatgacct
     1561 actgcgcacg catcgttgct atcgggctgt ttgtcgccga cggactggca gacaggacgc
     1621 ttatcacctt tgacagcaac ggaccgaaag actgcctgga ctattccctg tcgctggaac
     1681 cctcattccg cgaagacgac ctgctgatcc tgcccggcga tcggctgctg ctggcgagcc
     1741 atgactacct ggtgaccgcc gtgggtaaag aggcacagca gtctttgttt gaacttggcc
     1801 acctgaccat ggtgtttaac ggcagcctga atgcctgcca ttcgggagcc gttcatctct
     1861 ccggcccaat ccctaaactt cacgacctgt acggtgaact gctgattgag gagggacggc
     1921 cataagcgct gccttgaatc catccacaac aacaaaagag aagacattat ggaactcaac
     1981 agaagtctga aagcccgtca cctgacgatg atttccgtgg gaggcgtgat tggtgccgga
     2041 tttttcctcg gcgcaggcgc ggccattgcc cgtaccgggc cgtcggtggt gctggcctac
     2101 cttatcggcg gtgtcatcac cctgttcatt atgctgttgc ttgccgaaat ggcggtcgcc
     2161 aggcccgtct ccggttcctt tgaatcctat gcccgcaccg cctttggccc atggtgtggc
     2221 tttatcaccg ggtggaccta ctggctggcc ttcctgattg gcccggcgtc ggaggccatc
     2281 gctgcaggca ccttcctgaa cctgtggttt cccggagtcc ccgtctggct gttttgcctg
     2341 gtgatcgctt cgtcgctgac tgccatcaac atggtcggcg ttcatttttt cggtgaagtg
     2401 gagttctggc tgagcctggt gaaagtcgtg gcgctgctgg cctttatcgt cgcgggctgc
     2461 tacagcctgg gactggataa cgcctcaccc gccagcatca gtcatccgac cgacagcggc
     2521 agcttttttg ctggcgggct gcccggattt ctcggcgcga tgttaatggt gattttctct
     2581 ttcggcggga cagaggccat cggcaccgcc gcgggtgaaa gcgagaaccc acagcgtgat
     2641 attcctcgta cgctgcgtgg caccgtggtg cggatcctgt tgctgtatgt cggctcattg
     2701 accgttctgc tgctcgttct cccctggcag caggcaggtg tcagcagcag cccgtatgtc
     2761 gaagcttccg gcattctcgg tggccatatg gcgcggcata tcatgaactt tgtggtgctg
     2821 accgccgcgc tctcctgcat cgataccggg gtttacgcca cctcgcggat gctgcacgcc
     2881 atggcgtctg acggctattt cccggcctgg tttgcccgcc tgcacccggc acataaaaca
     2941 ccggataacg ccattcttgc cagcagcctg gtgctgttta ccggcgtcat catcgccatt
     3001 ctctcgccag atgcgtatgt catcctcgcc gggatttccg gattcggctt tctgttcacc
     3061 tggctgatga ttgccttaag ccagcccacc ctgcaccgaa tagccctgca cgacggaacg
     3121 ctgaaataca aggctccggc cgcccgctac atccagccac tcaccgtggg gctgattgcg
     3181 ctgatcatgg ccgggcaatt tttcaccgac ggcggcggca ttaccctggt taccgtcctg
     3241 atctggctgg ccctcgccag cgcgtactac atctttgtcg tccgaaaaaa caggaggctg
     3301 tccgatgaaa aaagtcatgc tcaatgaact gactggccgg gaaatccgcg agctgttgca
     3361 gtctggtaag gaactcactg ctatcgtcaa cttcggcagt tgtgagaacc acgccgatca
     3421 cctgccactc ggccccgata tgtttgtacc cgaaatcgtc gcctgccggg tggcgcagcg
     3481 ggtggataat acccttgtgc tgccctgcac gccgttcggc acatccgttc actacaatgc
     3541 ctatccactg gcggtcaccc tacgctacga gaccaatatc gcgctggcgg aagatatctt
     3601 catcagcctg ctgcaccacg gtattcgccg gatcctggtg attaacggcc atgacggtaa
     3661 tattccggcg attgaaatcg ccgcccgcaa tgtgaaggcc cgacatacgc aggcagcgat
     3721 tgtcgtggtt ccggcgtggt gggatctggt gggacatcat ctcgggaagg gatttttcga
     3781 ctcatgggat aacaccggac taggacacgg cggcgagggc gaaacgtccg tcatgatggc
     3841 gctgattccg gagctggtgg aactgaaata tgcgcagaaa cagatcccgg tggaggtgtt
     3901 cgctgattat ggcgtgaacc ggatctggga tatcggggaa atcagcgcca ccggagcaac
     3961 cggcgatccg cgctgcgcca cggcggaaaa aggccagcgg atcctcgcct gtctggtgga
     4021 tgtgctttgc gcgttgctga gaaagctgga tgcagctaac tggcaatatg cgttaaataa
     4081 agacgcaaac atgtaatcga tatgctcggg caacctgtgg agtggctttt gcctttccac
     4141 aggttacccg gaagcgatat cccaaccggg taaccgattg gtgttagccc agaatctatt
     4201 atacttcaat gagtaatatt attgctgaga tgaatgacag ttcaggttta gtcaccttac
     4261 caccaacaaa ttctgcacat ttactcctga gatttgctaa ttcatctttt aatttttcgt
     4321 tgctgtgttg cttaagatat atttgtcctc ttagctcaac ttcagtactt gccttcaaaa
     4381 attcctttcg gctttgatag gtgg
//
LOCUS       DAZKGF010000269         4396 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZKGF010000269 DAZKGF010000000
VERSION     DAZKGF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.269"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..314
                     /locus_tag="ACHOQV_004818"
     CDS             <1..314
                     /locus_tag="ACHOQV_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633061.1"
                     /translation="FMSEITVKNISPAVAGWWAKFRDDDGTEWYSPIAAWALCEVAHC
                     NTGCVYQEILPVLPGEAGMEPHYSDCGACECLYLPDKKFVHCGESWVFAWYPVDDNHQ
                     R"
     gene            333..782
                     /locus_tag="ACHOQV_004819"
     CDS             333..782
                     /locus_tag="ACHOQV_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9633062.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTAMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            799..1215
                     /locus_tag="ACHOQV_004820"
     CDS             799..1215
                     /locus_tag="ACHOQV_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFP9633063.1"
                     /translation="MNQQTTQQDQTTRRPTERPSWRHMTKRQRKNRRRINRLAEYWPE
                     LFGAETIKPLKTGIIRDVLEDAKSRELPIGHGVLCGALTSYIHTTRYLKAIVAGGPRY
                     GLNGQPCGEVTSDEKAVARGILKQRLEQMKQKNIQQ"
     gene            1235..1528
                     /locus_tag="ACHOQV_004821"
     CDS             1235..1528
                     /locus_tag="ACHOQV_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9633064.1"
                     /translation="MITDSVAQKLEERGLWRRAATRWAEVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQLVTFGDVKKAADRTLKEMGINPQDEWRSYSFSDAGDDLTLP"
     gene            complement(1979..2365)
                     /locus_tag="ACHOQV_004822"
     CDS             complement(1979..2365)
                     /locus_tag="ACHOQV_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633065.1"
                     /translation="MRLTQYKKEILSYFTPDNLAWVSVEIGNPPFDVSGVAYLLHGMD
                     SFNSRYQIESTRRTLEAMVRDGLLERINVFERREGAGDGVRRKVARYGLPGHSVVVRD
                     GEGRNGAIDGECIRINATDVVLVDGQ"
     gene            2951..3112
                     /locus_tag="ACHOQV_004823"
     CDS             2951..3112
                     /locus_tag="ACHOQV_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633066.1"
                     /translation="MTERSPTEIIHGIIEEQEERELILMIEEQRERQRKQEATLPESV
                     SAENQADVP"
     gene            3550..3942
                     /locus_tag="ACHOQV_004824"
     CDS             3550..3942
                     /locus_tag="ACHOQV_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9633067.1"
                     /translation="MRDIQQVLERWGAWAANNRENVTWSSVAAGFKGIILPKITSRPQ
                     CCDDDAMVICGCMARLNRNNSDLHDLLVDYYVLGKTFMALARKHGCSDGHIGKRLQKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            3979..4215
                     /locus_tag="ACHOQV_004825"
     CDS             3979..4215
                     /locus_tag="ACHOQV_004825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633068.1"
                     /translation="MEKYGLPINHEKRNRLIIMPRDKGRCTLNGHNDHSDHQGNHNEH
                     RYGWNFYPIGGKGAYLNHHYGDQGYVKPTSLIKG"
BASE COUNT         1248 a         1034 c         1207 g          907 t
ORIGIN      
        1 aattcatgag tgaaattact gttaaaaata tctcacctgc ggtagctggc tggtgggcga
       61 agttcagaga cgacgacggc acggaatggt acagcccgat tgctgcatgg gcgctttgtg
      121 aggtcgccca ctgtaatacg ggatgtgtgt atcaggagat actacccgtc ttgccaggtg
      181 aagcaggcat ggagccgcat tattctgatt gtggggcgtg cgagtgcctt tatctgccgg
      241 acaagaagtt tgttcactgc ggggagtcat gggtatttgc ctggtatccg gttgacgata
      301 accatcaacg ctaaaaccag tgaggtggac gtatgacagc acaaattgca gcttacgggc
      361 ggctggtgga cgacccgcag gtaaaacaga ccagcaaggg aacagcgatg acgctggcgc
      421 gtatggcggt atctttgcca tgcagtcagg cacaggacgg acaggcgacg ttatggctat
      481 cagtcctggc atttggtaag caggccgacg cgctggcgag acatgaaaaa ggcgacctta
      541 tcagcgtggc gggtaacatg cagatcagcc agtggaccgg acagaacgga gaaatacggc
      601 agggctggca ggttatcgct gacagcgtaa tcagtgcccg ttcggcgcgt cctggcggga
      661 agcgtgggca acaggggaag gcgaccgaag cactggaaag agcaaaacga cagaccggac
      721 agcagggaaa tcagccacca gcgggaggtg atgacccgtt ctctgacgat attcctttct
      781 gaatgacagg agcacaacat gaaccagcag accacacaac aggaccagac cacacgcaga
      841 cccacggagc gcccatcatg gcgacacatg acgaagcggc agcgaaaaaa ccgcaggagg
      901 ataaaccgac tggctgagta ctggcctgaa ctgtttgggg cagaaactat aaagcccctg
      961 aaaacgggca taatacgtga tgtgcttgag gacgctaaat cacgggaatt accgattggt
     1021 catggcgtat tatgtggcgc attaacctca tacatccaca cgacgcgata cctgaaagcc
     1081 attgtcgctg gtggccctcg gtatggactg aacgggcagc cttgcgggga agtgacgtcg
     1141 gatgaaaaag ccgtagcccg tgggatactg aaacagcgac tggagcagat gaagcagaaa
     1201 aacatccagc agtaatcagc cggaggtaaa gccagtgata acggacagcg tggcacagaa
     1261 acttgaggag cgcggtttat ggcgacgtgc ggcgacccga tgggctgagg ttcttcttca
     1321 cgcagaaaca gacagggaac gcgaggaagc tgccagacgt cgggcgatat gtatcattaa
     1381 atcccgtcgt atgcctgaac aactggtcac ctttggcgat gtgaaaaagg ccgcagacag
     1441 aacgctgaaa gagatgggga ttaatccaca ggatgaatgg agaagttaca gcttcagtga
     1501 tgcaggcgac gatttaacgc taccgtgata cgcatctcat cgccgtattt gctcagttat
     1561 cgcgattcgc tataatcacc accgaaaggt cgttgatggt gttcgcctcg ggcaactgga
     1621 gcccgacagc atgaagccag ccagcggctt ttcggtactc attttgatgt gtacttgtca
     1681 cacacatcgg gatgagtgaa cgacgaaaac atcacggtga tttcgtcatg ccgcgataag
     1741 aatgttcccc tttgggcaac tggagccctc tgtgcagaag gttatcgcgg caagttgtga
     1801 taaaaacgcg cctctttggg caattgaagc cctctgtaaa gcggcttatc acaacaggct
     1861 gtagcgggtg ctcatctctg ggcaactgga gccctctgta cagaggcccg ttacggctga
     1921 ccctgaagga tttctgccac gtgcaactgg agcacccagc ggagttattc agggtttttt
     1981 attgcccgtc caccagcacc acatctgtag cattgatgcg tatacattcg ccgtcaattg
     2041 caccattccg gccctcaccg tcacgcacca caacagaatg accaggcagg ccataacgcg
     2101 ccacctttcg acgaacacca tcaccagcgc cctcacgacg ctcaaacaca ttaatccgct
     2161 caagaagacc atcacgcacc atagcttcca gcgtgcgcct tgttgattcg atctgatacc
     2221 ggctgttgaa tgaatccata ccatgcagca ggtaagccac accggagaca tcaaacggcg
     2281 ggttgccaat ttcaacgctg acccacgcca gattgtcggg agtgaagtaa ctgaggatct
     2341 cttttttgta ttgcgtcagt ctcatagtga cctttcttgt tgtaggatga atccctcata
     2401 caataagcca taaggcaaat tttgaaagga cagaatcggc ggggtacaga tgatacgtga
     2461 tgacaaggct gaagcgctgg agaataacga ccaggtaagt tggttaatat ccggcaggct
     2521 gtaataagaa ccgaacatag cggcctccgt caccattatc agacagtagc ggtagtcctt
     2581 ccatcacagc aaaatgctac ccacttcaca gccccttaga agcctcttcg atggggtgaa
     2641 aaatccctga aaaaatgcgc aaaaaagtgc gcaaatcgat cataaaaccg atcacaaaat
     2701 cgatcacaca gaccagcaga gaaaagccag gtatgacgcg gcttgcaggg gggaaaggcc
     2761 accggaaaaa gtgcgcattt ttctcaaaaa cgaaacgcaa caggaagcgc aaaacgaaac
     2821 cgatacgcga tgatcgaaaa agcgcgcaaa tttaccggat tcgggtaagc aattttgcct
     2881 gatttgggca aaatacggtg tcgccgtaac agtgacattg acaccaaaac agtgacacac
     2941 aggagggccg atgaccgaac gttcaccaac agagataatt cacgggatta ttgaagagca
     3001 ggaggaacgc gagttaatcc tcatgattga agagcagaga gaacgccagc gcaaacagga
     3061 ggccacgctg ccagaaagcg tgagcgctga aaatcaggct gatgtgccgt gatggtgtcg
     3121 aactgagtaa cttaaagtca ctctgtgacc agttttaaac gagttgcaaa aagagccgga
     3181 gaaaaaccac gaatctgggg tttttactgg cgcggtgtac acggtggaac gcatagcgtt
     3241 acctgactta ttgcattgta aaaagttaga aaggcactgc ctgtaagatg ggatgttgtg
     3301 agtagtttaa agctacccgc aaattctttc gttgtgctgg cacaggcggt taaaccgtcg
     3361 gtaactggat aatccccatt gggggaaagc tggctacacc cctcacatct gaggactgtt
     3421 aaccagcaag ccggaatata ttcacaaact acgaaatttt cgtagtttaa ctttcatcca
     3481 gtacatcccc gaaaacacgg gtatgtacgg agttaccgtt accaataatt attcgcgagg
     3541 tgattgttga tgcgtgatat tcagcaggtt ctggaacgct ggggagcgtg ggcggcaaat
     3601 aatcgcgaga atgtgacctg gtcatccgtt gccgcaggat ttaaggggat tattctgcca
     3661 aagataacat ctcgtccgca gtgctgtgac gatgatgcga tggtgatatg cgggtgcatg
     3721 gctcgcctta acaggaacaa cagcgatctg catgacttgc tggttgatta ctacgtgctg
     3781 gggaaaacgt ttatggcgct ggcacgtaag catgggtgct ctgatggaca tatcggaaag
     3841 cggttacaga aagcggaagg tgttgttgaa ggcatgctga tgatgctgga tgtgaagctg
     3901 gagatggatc ggtatgttga gcgcgattca gtgacggcat aacgcgcata aaaaaaccgc
     3961 agccatgagg aaactgcggt ggagaaatac ggcttgccaa tcaatcatga aaaacgtaat
     4021 cgcctgataa ttatgccacg agacaaagga cggtgcaccc tgaatggtca taatgaccac
     4081 tcagaccatc aaggcaacca taacgaacat cgttacggtt ggaactttta ccccattggg
     4141 ggaaaaggtg catacctgaa tcatcattat ggtgatcaag ggtatgtaaa gcccaccagc
     4201 ctgattaaag gttaaccgga aaaagccagg tatggaggga gtgattagcc acgaacaagc
     4261 cggacaccac cagcagaagg tattatgatt ttcgtaatac ctttagcaaa gcgtgaatca
     4321 tcattacggt agggatggtc atactgacca cacctccccc caccggaaca gggaggatga
     4381 cctacgaaaa tttcgt
//
LOCUS       DAZKGF010000270         4388 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZKGF010000270 DAZKGF010000000
VERSION     DAZKGF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.270"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(31..648)
                     /locus_tag="ACHOQV_004826"
     CDS             complement(31..648)
                     /locus_tag="ACHOQV_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633069.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDVGFEKDLGYPCAIMPALVGYHSQYVAPYYQ
                     PIYTTSTCATGNKIYSCEHYSGAASWSNFSRYIDKVLVINASMYD"
     gene            complement(654..932)
                     /locus_tag="ACHOQV_004827"
     CDS             complement(654..932)
                     /locus_tag="ACHOQV_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633070.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(947..2215)
                     /locus_tag="ACHOQV_004828"
     CDS             complement(947..2215)
                     /locus_tag="ACHOQV_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9633071.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNGEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGALREETT
                     RTIGGIETRITTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            complement(2212..3837)
                     /locus_tag="ACHOQV_004829"
     CDS             complement(2212..3837)
                     /locus_tag="ACHOQV_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633072.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWPDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEITLRTPGTAVQLTLAPVPLQNASIKRRRIYRSASGGGEANFLLVAEL
                     DASVLSYTDKILAKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            complement(4198..>4388)
                     /locus_tag="ACHOQV_004830"
     CDS             complement(4198..>4388)
                     /locus_tag="ACHOQV_004830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633073.1"
                     /translation="GTGITDKKMGDHHLPHELSQLYTCEVFTMRINTVTESAGLDVME
                     IMFPAHPVDMDNFSDFRK"
BASE COUNT         1143 a         1192 c          908 g         1145 t
ORIGIN      
        1 aaaaagtttt tattaatata gattttctta tcaatcatac attgatgcat taataaccag
       61 taccttatct atatatctgg aaaagttaga ccatgaggca gcaccagaat aatgttcaca
      121 ggaatatatt ttatttcctg tagcacaggt ggatgtggtg taaatcggtt gataataagg
      181 tgctacatac tggctatgat accccaccag tgccggcata attgcacatg gatacccaag
      241 gtccttttca aatccgacgt caacaggtat taacttcgcg tcaagcagca tcatttcgcc
      301 atgataaatc atctcaccgt ccgggttata catggcgata ccatactcag aaggtggagt
      361 aaccatattt gcgaaagcat aaaccgtcgt aacaccaggg ttcgttcccc tgacaattac
      421 atgaagcctt agtgcatgat atccatcaat ctgttcatgc gtgtacatga catcggcctt
      481 cttctctgtt ctgataaaga aaaaacaact tttgcctgac gggattgatg ttttaaaaaa
      541 agacttttca gtcgccggta tggtgccttt gttgatcaga cactgcggcg taaaacctgg
      601 acttatccat acgctgccat ccggcttcat aatgctcaaa ccatacatga cacttatccc
      661 cagaatgtat aaatataaga tcccataccc tgctcaagat tcgaccacgt caccgtattg
      721 ccattaatgg ttatcttcgg tactttccga tccgcaaata cattattcca gggaaataaa
      781 caacatacag cctgcaatga tttcccgtcg ggtttattcg tgtacgtctt tgagccagac
      841 tccgctgtaa atctgtccag gaaaaatacc ggagtcagca cgcccgtaac attaacgtta
      901 tttctgttat aaatggcaaa accgtattcc aatactcacc tcctgatcag cgtaatctgc
      961 ctatgcgaac agccagtcgt ccattctgat cataaacctc aattttatca ttgcggatca
     1021 ccagtcctac attctgatta gagtaacgaa ttgtcagttg cccttgtgac gtaacactga
     1081 aaaggcctcc aatattcagg ttaccctgag aatcaacctg aaagtttccg ttctgaataa
     1141 cggcactccg gataactggc gaagtgatac ttaccccggc ttttacttca tccgccacaa
     1201 ccttccgcga caccagtgtt tcaatcactg cgtcataaat catcgctttc gggatcacta
     1261 ccttgccacc tgataccgca aacggatagg ctgtgttatc cggattattt gggtcaaaga
     1321 caaacaactg cgacgcagaa attgcaacct gacttacagg cctgccttca ctgtcttttc
     1381 cggcgacaat cccgatcccc gcagtgatac catcaactcc cgcttttttt gaccacattg
     1441 ccagaaacgc ctcaccgcct tctttatcca gtttagtgat gcgcttgtcg acctcattaa
     1501 gcgattcact ggttgacgaa tccagtgtgg ttatgcgggt ttcaatacca ccaatcgttc
     1561 ttgtcgtttc ttccctgaga gcccctacaa cttcggttgt cttaattgca gcatccttta
     1621 cagcctgccc ctgcgcgttt tttatttctt tacgcagctc ggacacaacc ggcgactttg
     1681 cagcctcatc gcggatctgg tcaatgatgg ccttcacacc gatctgtgtt tgtgcctgtg
     1741 tgcctttttc accattccat ggacctttca ctcctgccgc gttaacaaaa cgtatccagt
     1801 aaaatcccga ccagccaggg tcaaccggat cgccataaac ctgcccggga gttgtggcaa
     1861 ccagtaccgc atccgcaagg tcatcctccg taccccgcca gatttcagtc agtgaatgtc
     1921 cgcgataatt aggcatatcc cattcaagaa gaaccgagcc aaatccaccg gtcgccttaa
     1981 aattcagcgg ttttgtggga aaatcaacag tcattaaagt actgtcaatc tcaatacccg
     2041 gattcagtgc atatgaggca ccacccgatg ttcgacgccg ggcgagttta agaccaacca
     2101 gttcctcacg ggtcacaaat gcgtgacgtc cgtcaccacg ctgcccggtg ccaatttcca
     2161 tattctccac aacagtggat aaatccttcc ccgcacgcca cggttttctg gtcataccgg
     2221 catctccgac attgatgtac tcagggttat tcgttccacc tgcccgaatc cggataccat
     2281 cacctgccag ttttgcccgg ttgctgccgg aagtctcacc acacttccct taaacgtacc
     2341 cggcgcaaaa tgaatcacag gaacatcatc agccataatg gtgatcccca cccgctcagg
     2401 cgccggagat ttcaccctga tacaggaaaa agaggttctt tcaggtaatg aaaaaatttt
     2461 tgaatgccac cttatcgtgg agggcagaga gcccccggca agcactgaca ttttgtctcc
     2521 tgtcaccacg cgcatcatat ctttcgcgag atcaacccat gcgcagtcaa acggtgtact
     2581 gagataacgg atatccatgt tcaccggact gaatacaaac acatcctgct taccatccgg
     2641 tttcgtgtaa caggcaatgt actcaccacg ccaggaataa gccacaatgg acgccgggtt
     2701 aaactgactc tgccactgtt caggtgaaat aatcttttcc gttgccagtg ctgtattacc
     2761 gtttacatca acagatacca gaccgtttgt cccggcatag agtacgaatc cctccatcgc
     2821 caccatactt cttcggctca ggcatgcctg catggaagga attctggagc cagaaattgt
     2881 ggaaggcgat acaccactga acagataagg ctcccccttt gtcgccacca ccagtgacgt
     2941 tcccagcgga cagatagcca caatatcttc tgccgtcgtg tgacgattca cttccggcca
     3001 tgcatacggc agatacgctt ccgaaaacat cacttcatta ccggcaaacc cggcggcaat
     3061 accattagcc atcaggcaaa ggcctgtcat attctctggc ggcggcaggt aatcccatgt
     3121 cgccagggaa ggcccaaggt ttttcgccag tattttgtcc gtgtaactga gcacagacgc
     3181 atccagttca gccacaagta aaaaattcgc ctcccctcca cctgatgcag agcgataaat
     3241 ccggcggcgt ttaatactgg cattctgcaa tggcaccgga gccagcgtca gctgtaccgc
     3301 agtccccgga gtacggagtg ttatttccag agacgccgga cctggcggac cttcttcacc
     3361 ataatctgag acaaaggttt ccgtataaaa ccgggtttca tcatcattcg ggttatcgtc
     3421 agaaacatca ccgccctgct gaacagtaca gacaggtgct gtcgtcggcg cggggatccc
     3481 cagacgatac gatgatgtcg ggtgattccc gtcccctttt gtggcaatgg tcgcatccgt
     3541 cactttagga aaacgcccgt cagtgtagta aatacgcccg tgggggtcct gagcgatcgg
     3601 actgcggatc acatccacca catccggcca tgcaaaccag aaatcgtcac ggtaatgaaa
     3661 aatggttttc ggcttaatcg cgaatgtttt ctcagcctct gacatctggt gttcaggcgt
     3721 gatcactcca tagcgaaaat gacagttttc tgccagtaca gcagaatgat ctggcagcat
     3781 agatgcaata acgcccggca tcatcccgcg catagtggtt atatcgatat aaggcatggt
     3841 tgtctggtat ccgaaaattt gaatggcagg taattcactg aattgagggt gttaaggcgg
     3901 cataaactgc gcctgtgaag aatacagact tcaggaaatc cgaaaaatct ctcttatcag
     3961 agaagagtgt cagaaatacg atcggcatca ctgtttctgc aaacttccct gtggcagtct
     4021 ttattcgcca tataacgtag cactaaaggt cttcattaat ttttccattg tagttactcc
     4081 ttccttgtac cgggggttgg tcatgccccc gttttttatt ccctctgctc ctttaatcgc
     4141 actgccaggc gccacaacac acatcaactg tggctggtat ggaaacacca aaaactctca
     4201 cttccggaaa tctgaaaaat tatccatatc aacaggatgc gccggaaaca tgatttccat
     4261 cacatccagg cctgcacttt ccgtgacagt atttattcgc atagtaaata cttcacaagt
     4321 gtataattga ctcagttcat gggggaggtg atggtccccc atctttttgt ctgttattcc
     4381 tgtgccca
//
LOCUS       DAZKGF010000271         4384 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZKGF010000271 DAZKGF010000000
VERSION     DAZKGF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.271"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            188..403
                     /gene="bdm"
                     /locus_tag="ACHOQV_004831"
     CDS             188..403
                     /gene="bdm"
                     /locus_tag="ACHOQV_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFP9633074.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            505..642
                     /gene="sra"
                     /locus_tag="ACHOQV_004832"
     CDS             505..642
                     /gene="sra"
                     /locus_tag="ACHOQV_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFP9633075.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            799..2496
                     /gene="maeA"
                     /locus_tag="ACHOQV_004833"
     CDS             799..2496
                     /gene="maeA"
                     /locus_tag="ACHOQV_004833"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP9633076.1"
                     /translation="MEPKTKRQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            2630..3640
                     /gene="adhP"
                     /locus_tag="ACHOQV_004834"
     CDS             2630..3640
                     /gene="adhP"
                     /locus_tag="ACHOQV_004834"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFP9633077.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3826..4104
                     /locus_tag="ACHOQV_004835"
     CDS             3826..4104
                     /locus_tag="ACHOQV_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9633078.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            4104..>4384
                     /locus_tag="ACHOQV_004836"
     CDS             4104..>4384
                     /locus_tag="ACHOQV_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP9633079.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVT"
BASE COUNT         1140 a         1068 c         1143 g         1033 t
ORIGIN      
        1 tattgcatca atatcaatca actcatcaca ataataatac ctatcaatat catggagtta
       61 aaatataaaa atcatcaacc aggactaatc ttaacaacga agcggcaaat atttgccatg
      121 ctgaatgtgc tttataaatc tgcgatccgt agcagacacc ataaatacac agacacggag
      181 aatcactatg tttacttatt atcaggcaga aaattcaaca gcagaacccg ctctggttaa
      241 tgccattgag caaggccttc gggcagagca cggtgttgtc actgaagatg acattctcat
      301 ggagctgacc aaatgggtgg aagcatctga taacgacatc ctcagtgata tctaccagca
      361 aacgatcaat tatgtggtca gtggtcagca ccctacgctt taaggtgcta tgcttgatcg
      421 gcaacctaat ttaggggttt agcacgtgtt tcttcgctac ggcgatgttg tccttaaaac
      481 tagtaacagg attgaggagt taaaatgaaa tcgaaccgtc aggcacgtca tattcttgga
      541 ctggaccata aaatttctaa ccagcgcaaa atagttaccg aaggtgacaa atccagcgtg
      601 gtaaataacc caacaggcag aaaacgcccc gctgaaaagt aattcataac catcagtcct
      661 caatgacgat taaacaccat tgcctgcgca atggtgtttt tgtttttatc tgctttatac
      721 ttggggccga cgccctggcg gtaaagcaaa gacgataaaa gcgtgccagg gatggatatt
      781 caaaaaagag tgagtgacat ggaaccaaaa acaaaaagac agcgttcgct ttatattcct
      841 tacgctggcc ctgtactgct ggaatttccg ttgttgaata aaggcagcgc cttcagcatg
      901 gaagaacgcc gtaacttcaa cctgctgggg ttactgccgg aagtggtcga aaccatcgaa
      961 gaacaagcgg aacgagcatg gatccagtat cagggattca aaaccgaaat cgacaaacac
     1021 atctacctgc gtaacatcca ggacactaac gaaaccctct tctaccgtct ggtaaacaat
     1081 catcttgatg agatgatgcc tgttatttat accccaaccg tcggcgcagc ctgtgagcgt
     1141 ttttctgaga tctaccgccg ttcacgcggc gtgtttatct cttaccagaa ccgccacaat
     1201 atggacgata ttctgcaaaa cgtgccgaac cataatatta aagtgattgt ggtgactgac
     1261 ggtgaacgta ttctggggct tggtgaccag ggcatcggcg ggatgggcat tccgatcggt
     1321 aaactgtcgc tctataccgc ctgtggcggc atcagcccgg cgtataccct tccggtggtg
     1381 ctggatgtcg ggacgaacaa ccaacagctg cttaacgatc cgctgtatat gggctggcgt
     1441 aatccgcgta tcactgacga cgaatactat gaattcgttg atgaatttat ccaggctgtg
     1501 aaacaacgct ggccggacgt actgttgcag tttgaagact tcgcacaaaa aaatgcgatg
     1561 ccgttactta accgctatcg caatgaaatt tgttctttta acgatgacat tcagggcacc
     1621 gcggcggtaa cggtcggcac actgatcgca gccagccgcg cggcaggtgg tcaactgagc
     1681 gagaaaaaga ttgtcttcct cggcgcaggc tctgcggggt gcggcattgc cgaaatgatc
     1741 atcgcccaga ctcagcgtga aggattaagc gaggaagcgg cgcggcagaa agtctttatg
     1801 gttgatcgct ttggcctgct gaccgacaag atgccgaacc tgctgccttt ccagaccaaa
     1861 ctggtgcaga agcgcgaaaa cctcagtgac tgggataccg acagcgatgt gctgtcactg
     1921 ctggatgtgg tgcgtaatgt aaaaccagat attctgattg gcgtctcagg acagaccggg
     1981 ctgtttacgg aagagattat ccgtgagatg cataaacact gtccgcgtcc gatcgtgatg
     2041 ccgctgtcta acccgacttc acgcgtggaa gccacaccgc aggacattat cgcctggact
     2101 gaaggtaacg cgctggtcgc cactggcagt ccgtttaacc cagtggtatg gaaagataaa
     2161 atctacccta tcgcccagtg taacaacgcc tttattttcc cgggcatcgg gctgggcgtt
     2221 attgcttccg gcgcgtcacg tatcaccgat gagatgctga tgtcggcaag tgaaacgctt
     2281 gctcagtatt cgccgctggt cctgaacggc gaaggtctgg tactaccgga actgaaagat
     2341 attcagaaag tctcccgcgc aattgccttt gcggttggca aaatggcgca gcagcaaggc
     2401 gtggcggtga aaacgtctgc cgaagctctg caacaggcca ttgatgataa tttctggcac
     2461 gccgaatacc gcgactaccg ccgtacctcc atctaagcct gcgcccggtg gtgaaagctg
     2521 ccgggcaatt ttcctctccc tttttcagat ctcatccata ctgggtagtg gcgaataaat
     2581 ctcatttgcc tcacctgcta tgcagaacat catccgaaaa ggaggaacta tgaaggctgc
     2641 agttgttacg aaggatcatc atgttgacgt tacggataaa acactgcgct cactgaaaca
     2701 tggcgaagcc ctgctgaaaa tggagtgttg tggtgtatgt cataccgatc ttcatgttaa
     2761 gaatggcgat tttggtgaca aaaccggcgt aattctgggc catgaaggca tcggtgtggt
     2821 ggcagaagtt ggcccaggtg tcacctcatt aaaaccaggc gatcgtgcca gcgtggcgtg
     2881 gttttacgaa ggatgcggtc attgcgaata ctgtaacagt ggtaacgaaa cgctctgccg
     2941 ttcagttaaa aatgccggat acagcgttga tggcgggatg gcggaagagt gcatcgtggt
     3001 cgccgattac gcggtaaaag tgccagatgg cctggactcg gcggcggcca gcagcattac
     3061 ctgtgcgggg gtcaccacct ataaagcggt gaaactgtca aaaattcgtc ccggtcagtg
     3121 gattgctatc tacggtcttg gcggtctggg taacctcgcc ctgcaatacg cgaagaatgt
     3181 ctttaacgcc aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga
     3241 aatgggcgct gatttagcga ttaactcacg cactgaagac gccgccaaaa ttgtgcaaga
     3301 aaaaaccggt ggcgctcacg ccgcggtggt gacagcggta gctaaagctg cgtttaactc
     3361 ggcggttgat gctgtacgag caggcggtcg tgttgtggct gtcggtctgc cgccggagtc
     3421 tatgagcctg gacatcccac gccttgtgct ggatggtatt gaagtggtcg gttcgctggt
     3481 cggcacgcgc caggatctaa ccgaagcctt ccagtttgcc gccgaaggca aagtggtgcc
     3541 gaaagtcgcc ctgcgtccgt tagcggacat caacaccatc tttaccgaga tggaagaagg
     3601 caaaatccgt ggccgtatgg tgattgattt ccgccgctaa gaggcctttg ctgcgactgc
     3661 catgttcggg tcgcagcctg ttttgcgaga cgctttccgc aatttcactc ttcttctttt
     3721 cctcccccat atttcccctc acaattcgca aaaacgtagc gaaatttgtt gcgctgcacg
     3781 gaacaatagt gatagtgttc cttttgaagg aacaaggatg aagagatgat aatgaatttc
     3841 agacacaagg gattgcgtga cttgtttctt ctcggcaaaa cctccggcgt tataccgacg
     3901 caagtcaaac gattacgcca ccgactcgct gtgattgatg cagcaagctg tcttgctgat
     3961 atcgatatgc ccggttaccg actacacccg ttaagcggcg atcgcgatgg aatttgggcg
     4021 atatctgtct cgggcaactg gcgaatcaca tttgaattcg tcaatggcga tgcatacata
     4081 ctggattacg aggactatca ctgatgaaaa tggccaatca tccccgcccg ggggacatta
     4141 ttcaggaatc actggacgaa cttaatgtca gcctgcgcga gtttgccaga gcaatggaaa
     4201 ttgcgccctc aacggcaagc cgattgctga ccggaaaagc ggcgctcact ccggaaatgg
     4261 cgattaaact ttccgtggtg atcggcagtt cgccgcaaat gtggctgaat ctgcaaaacg
     4321 cctggagcct ggcagaggct gaaaaaacgg tggatgtgtc gcgactgcgc cgtctggtta
     4381 cgca
//
LOCUS       DAZKGF010000272         4359 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZKGF010000272 DAZKGF010000000
VERSION     DAZKGF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.272"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..79)
                     /locus_tag="ACHOQV_004837"
     CDS             complement(<1..79)
                     /locus_tag="ACHOQV_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFP9633080.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDET"
     gene            complement(149..934)
                     /gene="ygcW"
                     /locus_tag="ACHOQV_004838"
     CDS             complement(149..934)
                     /gene="ygcW"
                     /locus_tag="ACHOQV_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9633081.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGKTKEMIEKQGVEMDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            1253..2530
                     /gene="yqcE"
                     /locus_tag="ACHOQV_004839"
     CDS             1253..2530
                     /gene="yqcE"
                     /locus_tag="ACHOQV_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFP9633082.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSTNNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSIAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            2557..4035
                     /gene="ygcE"
                     /locus_tag="ACHOQV_004840"
     CDS             2557..4035
                     /gene="ygcE"
                     /locus_tag="ACHOQV_004840"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP9633083.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWTSLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYDHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTILGHITPQAALATHFPAGLSVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMIRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         1158 a          979 c         1035 g         1187 t
ORIGIN      
        1 ctgtttcatc ggtaattacg cgatctgcac caacaatttc ctttagctgg tcgacaatcg
       61 ctgcgcgaga taaagacata gtaattcctt cctgataata aaaatggaat aatcattaat
      121 aagagaaaac agaaaccacc gttatggatt agcgcactaa ataaccgcca tcgaccacta
      181 ataaatgccc gttgacataa ttcgatgcct gacttgcgag aaacacggct gcgcccatta
      241 aatcctgagt atcgccccaa cggtttgccg gaatatgatc aagaacgcgc tgattggttt
      301 ctggattact gcgtgtcgcc agcgtaatat ctgttgcata atagccaggg gcaataccat
      361 ttacctgaat attatattga cctagttcat cacaataagc tttggtgaac ccggcaagag
      421 catgtttagt ggcagaatat gcaggtgacc attgtccacc taagtaagag aacaatgaac
      481 agatattaat aattttaccg cttttctgcg ggatcataat ttttgccgct tcatagctta
      541 actcgaatgc ggcggtcagg ttcacatcaa tcatcgggtc ccagtcggca cgaccgaaat
      601 ccagcacctt attcagctta caaataccgg cattgttaac cagaatatca actgttccga
      661 aacgctcaca gcaagcagcg ataatcttct gcggcgcgcc ttctgcggtg atatccacct
      721 gcatgaagtc catctcaaca ccctgttttt caatcatttc ctttgttttg ccgttatctt
      781 taacgaagct tggaataaag atatttgcac cagctttggc cagcgccatg gcaaatgcct
      841 ggcctaaacc gctattcccg ccggtaacaa ttgcggtttt acctttcagg gagaaaaaat
      901 ccattgagaa cgcattgaga gattcgattg acatagttag ctccaatttc cgtaaggcaa
      961 aaaaaaagaa aggtaacttc ccccgcgaga gggaagttac ctttctcatt atctctggta
     1021 actggagata atttttatca taagggcata atcgctaatg tgatcgtaat cacagtgtta
     1081 tattcgaatt gtcaaataat tttaaaaagc agaagcgtga ttactctcac acaacaccac
     1141 tgctttccat gttatttctt attgcaatta ctagagacct gagaaaagta atccacctga
     1201 aagggcggct tacttttctc ttttttttgt ctattacaaa tgggaatacg atatgcaaca
     1261 caactcatat cgccgttgga taaccctcgc gataattagt tttagcggcg gcgttagttt
     1321 cgatctggct tatttacgtt atatttatca aattcccatg gcgaaattta tgggattcag
     1381 caataccgag ataggtttaa taatgagtac ctttggtatt gcggctatta ttctttatgc
     1441 ccccagcggc gttattgccg ataaattttc acaccgcaaa atgattactt ccgcgatgat
     1501 cattaccgga ttactgggtc tgttaatggc aacatatcca ccgctgtggg taatgctctg
     1561 tattcaggtc gcctttgcga taacgacgat tttaatgctg tggtcggtgt cgattaaagc
     1621 cgcctcattg ctgggcgatc atagtgagca agggaaaatt atgggctgga tggaagggct
     1681 gcgcggcgtc ggtgtaatgt cgctggcggt gtttaccatg tgggtctttt ctcgctttgc
     1741 gccggatgac agcgccagcc tgaaaacagt cattattatc tacagtgtgg tttacatctt
     1801 gttggggatt ctgtgctggt tttttgttag cgataacaac aacctgcgca gtaccaataa
     1861 cgaagaaaaa cagtcattcc agcttagcga tatcctggcc gttttgcgta tcagtaccac
     1921 ctggtattgc agcatggtga tttttggtgt cttcaccatc tacgccattc tgagttactc
     1981 caccaactat ctgaccgaaa tgtacggcat gtcgctggtg gcggcgagct acatggggat
     2041 tgtgatcaac aaaatcttcc gcgcgctgtg cggcccactt ggcggcatta tcaccaccta
     2101 cagtaaagtg aaatccccta cccgcgtgat ccaaatcctt tccgtactcg gcctgctggc
     2161 gttaactgcc ctgctcgtca cgaactctaa cccgcaatcg atcgcgatgg ggattggcct
     2221 gattttactg ctgggattca cctgttacgc ctcacgcggg ctgtactggg cctgcccagg
     2281 cgaagcgaga acaccgtctt acattatggg caccacggta ggtatttgtt cggtgattgg
     2341 attcctgccg gatgtcttcg tttacccaat tatcggccac tggcaagaca ccctgcccgc
     2401 agcagaagcc taccgcaata tgtggctgat gggcatggcg gcgcttggca tggtgattgt
     2461 ctttaccttt ttgctgttcc aaaaaattcg tactgctgat agcgcccccg caatggctag
     2521 cagcaagtaa gccaggaaaa ttacaggaga aaaatcatgt cgaaaaaata catcataggg
     2581 attgatggcg gaagtcagag cacaaaagtg gtgatgtacg atctggaagg taatgtggtt
     2641 tgcgaaggta aaggcttatt gcagccgatg catacgccag atgccgatac tgccgaacat
     2701 cctgacgacg atttatggac atcgttatgc tttgccggtc acgatttgat gagccagttt
     2761 gccggaaata aagaagatat tgtcggtatt ggtctgggct ccatccgttg ctgtcgtgcg
     2821 ttattgaaag ccgatggcac gcccgctgcg ccattgatta gctggcagga tgcacgtgta
     2881 acacgtccct acgatcatac caatccagat gtggcctatg tcacctcttt ttcgggttat
     2941 ctgacgcatc gcttaaccgg cgagtttaaa gataatatcg ccaactattt tggtcagtgg
     3001 ccggtggatt ataagagctg gacatggagt gaagatgctg cggtaatgga taagtttaat
     3061 atcccccgtc atatgctgtt tgatgtgcaa atgcctggca ccattcttgg gcatatcaca
     3121 ccacaagctg cgctggcgac acatttcccg gctgggctgt cggttgtttg taccaccagt
     3181 gataaaccgg tagaagctct gggagccggg ttactggatg atgaaactgc ggtaatttct
     3241 cttggcactt acatcgcatt gatgatgaac ggcaaagcct tgccgaaaga tccggtggcg
     3301 tactggccga ttatgtcttc tattccgcaa acattgctgt atgaaggtta cggtattcgc
     3361 aaaggcatgt ggacggtgag ctggctgcgc gacatgttag gcgagtcgtt aattcaggat
     3421 gccagggcgc aggatctttc accggaagat ttactcaaca agaaagcctc tagtgtgcca
     3481 cctggctgta atgggctgat gacggtgctg gactggctga ccaatccgtg ggaaccgtac
     3541 aaacgcggga ttatgatcgg ctttgattcc agtatggatt acgcatggat atatcgttcg
     3601 atactggaaa gcgtggcgct gacgctgaag aacaattacg acaatatgtg taatgaaatg
     3661 aatcactttg cgaagcatgt aatcattact ggcggcggtt cgaacagcga tctgtttatg
     3721 cagatttttg ccgacgtgtt caaccttccg gcacgacgta acgccattaa cggttgtgca
     3781 agtctggggg cagcgattaa taccgcggta ggtctggggc tatacccgga ttacgcaacg
     3841 gctgtcgaca aaatgattcg cgtgaaagat atctttatac cgattgagag caatgccaaa
     3901 cgctacgacg cgatgaataa aggcattttc aaagacctaa ccaaacatac tgatgtgatc
     3961 ctgaaaaaat cgtatgaagt gatgcatggg gaattgggga atgcggattc gatccagagc
     4021 tggtcgaatg cgtaagtgag gaaggccggg cgggaaactg cccggcctga acatacctga
     4081 atggttatcc ccgctgacgc ggggaacata agttcgacaa cttaaaaaat gataacaagt
     4141 ccggtttatc cccgctgatg cagggaacat aataaaaact ttaactagtt cggtttatcc
     4201 ctgtttttac aaggaatatt aactacactc gatgtcttta gaataaaaga caatacgaat
     4261 cgtactttat caattttcat ttaaagtatg aactggatat ctttattcat aagaacaatt
     4321 catcaacagc gtatagaggc ggttatgtaa aaccgctta
//
LOCUS       DAZKGF010000273         4329 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZKGF010000273 DAZKGF010000000
VERSION     DAZKGF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.273"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(158..847)
                     /locus_tag="ACHOQV_004841"
     CDS             complement(158..847)
                     /locus_tag="ACHOQV_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP9633084.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            952..1182
                     /locus_tag="ACHOQV_004842"
     CDS             952..1182
                     /locus_tag="ACHOQV_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9633085.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            1252..1791
                     /locus_tag="ACHOQV_004843"
     CDS             1252..1791
                     /locus_tag="ACHOQV_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFP9633086.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            1788..2807
                     /locus_tag="ACHOQV_004844"
     CDS             1788..2807
                     /locus_tag="ACHOQV_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9633087.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            2804..3505
                     /locus_tag="ACHOQV_004845"
     CDS             2804..3505
                     /locus_tag="ACHOQV_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9633088.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            3502..3804
                     /locus_tag="ACHOQV_004846"
     CDS             3502..3804
                     /locus_tag="ACHOQV_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP9633089.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            3872..4204
                     /gene="emrE"
                     /locus_tag="ACHOQV_004847"
     CDS             3872..4204
                     /gene="emrE"
                     /locus_tag="ACHOQV_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFP9633090.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            4252..>4329
                     /gene="ylcJ"
                     /locus_tag="ACHOQV_004848"
     CDS             4252..>4329
                     /gene="ylcJ"
                     /locus_tag="ACHOQV_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YlcJ"
                     /protein_id="HFP9633091.1"
                     /translation="MSLVLCFLLMSLFFMYSFVLSRLWRK"
BASE COUNT         1207 a          975 c         1068 g         1079 t
ORIGIN      
        1 ggggagggca aaagaaaacc cggcacggtg accgggattc ttacgccgtt aggtaaagat
       61 attattgcgg tggcttaata ttactaccta gagcaaagat aggaattagt tctttactga
      121 atgagcacaa tgcccagttg ataattttta attggtacta cccatgcttc ctatatgtct
      181 gcggcatgct cccaataacc ttaccgaaga tgaacacccg gttcatctcg tctttctcga
      241 tcgggtccca cggtgagtag cttttgttat cagagatgac cagcagctta tccttcatca
      301 tttgcaggcg ctttacatgg gctgtgtcgt cgtacagaaa cgcatagata ccatcaccgt
      361 cgaaagattt aactgtgata tcaacgaaca gcagatcacc tggttcgatc gttcctgaca
      421 tgctgtcacc acgcacgtta atgatgcgga tattttccgc cttcctacca tcgaacatgt
      481 gacgagcatc gtcaaacgag tactcaaccg agcgtagaac ttctacaaac tcacggttga
      541 tgactcccgg cccagcactg acttctatat caagaacgtc aatcttgaag tatttggaat
      601 ggctgacagt tgattgtatt ggttgcactg tactgtctga catatttcca acgccagaag
      661 ataaccattc tgcgcgcaca cccaaagcgt tcgcgatctc cacgatttta gttgtttgat
      721 tagctttccc tgtttcgatt ttctgaatag cagcctggct aaccccgacc aaatccccaa
      781 gcgccttttg tgtaaggcct cgcgctaatc tggcttcttt aagtctttct gagagtgttg
      841 ttttcatagt ccaaatgtac aaccaaggtt ttattccatc aaacgaaaat ggttgttgac
      901 taaaaacaac catagtttta atcttgattc aaattaacca cggaggttgt tatgaaccca
      961 gctatcaaaa cagcgatcaa tatcgttggt tcacaaaaga aactgggcgc tgcttgcgaa
     1021 gtttcacagc aggccgtcta taagtggctt cacaacaaag caaaggtatc ccctgaacat
     1081 gtcggcagca ttgttacggc tactggtgga gtagtgaagg cataccagat tcgcccggat
     1141 cttccgaagt tgtttccaca caccgaaaag aacgcagctt aaatttccat ttcacgctct
     1201 ttaacaataa gcaatcaact taacagtcaa ttcaaactaa aggagtcaat tatgcaacca
     1261 cttacatacc aacagactag cggatttagc ccgactgcgg tgataaatcg ttctcaaaca
     1321 aaacaggtgc caggccacga aaaaatccgt gatgccgtcc gcgcctggtc ggctgtagat
     1381 aatcaggatg tcgttgccac actcattgtg aatgagtatc gggagcaggg cggcggcacc
     1441 atcgatttcc ctgatgatgt cagccgtgca cgccagaagc tgttccgctt cctcgataac
     1501 aaattcgatt ctgaaaaata ccgaaataac gtgcgtgaac tgaccccggc aattctggcg
     1561 gtactaccgc tggaatatcg cggttacctg gttgagcagg atagcttcat ggctaggttg
     1621 gctgaaatgg aaaaggaact cagtgaggca aaacaggctg tcattctcaa cgcaccacgc
     1681 caccagaaac tgaaggaaat tagtgaaggt attgtgtcga tgtttcgtgt ggacccagat
     1741 ctggctggtc cattgatggc gatggttact accatgctgg gggcgatatg acaggttcag
     1801 aaatggcgaa agccggtctg ctggaacaga accgactttc aggtgcaaat cgtaacacac
     1861 tcattgcggg aggaattatg gcaaacactg ctgagatatt caattttcca gtgccggatg
     1921 cggcacaaaa ggagccgcgc gtggcagatc tcgatgatgg ttatacgcgc attgcaaatg
     1981 agttgctgga agctgtgatg ctggccggat taacacagca ccagcttctg gtctttctgg
     2041 ctgtcatgcg caaaacatat ggctttaata aaaaactgga ttgggttagc aacgagcaac
     2101 tttccgaatt aaccgggata ttgccgcaca agtgttctgc tgcaaaaagt gttctggtaa
     2161 agcgtgggat ttttattcag agcgggcgga ataccggcat taataatgtg gtcagtgaat
     2221 ggtcaacatt acccgaatca ggtaagaaaa ataaagttta cctgaaagag gtaaatttac
     2281 ctgaatcagg taaaaaaagt ttacccaaat caggtaaagg cgtttacccg aatcaggtaa
     2341 acacaaaaga caaactaaca aaagacaata taaaaccttt ttcgtccgag aattctggcg
     2401 aatcctctga ccaaccagaa aacgatcttc ctgtggtgaa accggatgct gcaattcaga
     2461 gcggcagcaa gtgggggaca gcagaagacc tgaccgccgc agagtggatg tttgacatgg
     2521 tgaagaccat cgcgccatca gccagaaaac cgaattttgc agggtgggct aacgatatcc
     2581 gcctgatgcg tgaacgtgac ggacgtaacc accgcgacat gtgcgtgctg ttccgctggg
     2641 catgccagga caacttctgg tccggtaacg tgctaagtcc ggccaaactc cgcgacaagt
     2701 ggacccaact cgaaatcaac cgtaacaagc aacaggcagg cgtgacagct agcaaaccaa
     2761 aactcgacct gacaaacaca gactggattt acggggtgga tctatgaaaa acatcgccgc
     2821 acagatgatt aactttgacc gtgagcagat gcgtcggatc gccaacaaca tgccggaaca
     2881 gtacgacgaa aagccgcagg tacagcaggt agcgcagata atcaacggtg tattcagcca
     2941 gttactggca actttcccgg cgagcctggc taatcgtgac cagaacgaac tgaacgaaat
     3001 ccgccgccag tgggttctgg ctttccggga aaacgggatc accacgatgg aacaggtgag
     3061 cgccggaatg cgtgttgccc gtcggcagaa tagaccattt ctgccatcac ccgggcagtt
     3121 tgttgcatgg tgccgggaag aagcatccgt taccgccggg ctgccaaacg tcagcgagct
     3181 ggttgatatg gtttacgagt attgccggaa gcgtggtctg tatccggatg cagagtctta
     3241 tccgtggaaa tcaaacgcgc actactggct ggttaccaac ctgtatcaga acatgcgggc
     3301 caatgcgttg actgacgcgg aattacggcg caaggctgcc gatgaactgt cctgtatgac
     3361 cgcgcgaatt aaccgtggtg aggcgatacc tgaaccagta aaacaacttc ctgtcatggg
     3421 cggtagacct ctaaatcgtg cacaggctct ggcgaagatc gcagaaatca aagctaagtt
     3481 cggactgaaa ggagcaagtg tatgacgggc aaagaggcaa ttattcatta cctggggacg
     3541 cataagagct tctgtgcaca ggacgttgcc gcggtaacag gcgcaaccgt aaccagcata
     3601 aatcaggctg cggctaaaat ggcgcgggca ggaatcctgg tcgttgatgg taaggtctgg
     3661 cgaacggtgt attaccggtt cgctaccaga gaagaacggg aaggaaaggt gagcacgaat
     3721 ctgattttta aggagtgtcg ccagagtgcc gcgatgaaac gggtactgag ggtatataaa
     3781 agaacatcaa tgggaacaca atgatgaaac aggtgagttg agttcaaact gtagtacaat
     3841 tctctccagt ttgaacagga aagaatatgc tatgaaccct tatatttatc ttggtggtgc
     3901 aatacttgca gaggtcattg gtacaacctt aatgaagttt tcagaaggtt ttacacggtt
     3961 atggccatct gttggtacaa ttatttgtta ttgtgcatca ttctggttat tagctcagac
     4021 gctggcttat attcctacag ggattgctta tgctatctgg tcaggagtcg gtattgtcct
     4081 gattagctta ctgtcatggg gatttttcgg ccaacggctg gacctgccag ccattatagg
     4141 catgatgttg atttgtgccg gtgtgttggt tattaattta ttgtcacgaa gcacaccaca
     4201 ttaaaataat ttgtttctaa acgactaaaa tatggaggct cttatattta tatgagcctc
     4261 gttttatgct ttttgttaat gtctttattt tttatgtatt cttttgtgct ttcaagatta
     4321 tggcgtaag
//
LOCUS       DAZKGF010000274         4307 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZKGF010000274 DAZKGF010000000
VERSION     DAZKGF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.274"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            377..952
                     /locus_tag="ACHOQV_004849"
                     /pseudo
     CDS             377..952
                     /locus_tag="ACHOQV_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
     gene            950..2029
                     /locus_tag="ACHOQV_004850"
     CDS             950..2029
                     /locus_tag="ACHOQV_004850"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP9633092.1"
                     /translation="MEIVPGEQPGESDILITRKQDKYWRLGFSLDDSGTRSTGRYLGG
                     ITFSLDNPFSLSDLLYVSATHNFPHYGGKGSKNYTAHYSVPFGYWQFSVTASDYDYHQ
                     TVAGAVEDYQYSGESQNLGMQLSRVLHRNGTQKTVLTYDVQARRSRNYINDTEIQVQR
                     RQTAAWRLGLQHRHYISQATLDAGVSWQRGTRWSGAQPAPEETFGEATALSKILHINA
                     SFNLPFTLAEQPFSYSFRYQRQLSNTPLTPQEQFAIGNRWTVRGFDGERTLNASNGWY
                     IRNDLAWRTPLPEQALYLGMDYGEVGGAGSEYLVGRHLAGGVVGLRGRLLSTDYDVFI
                     GRPFSKPAGFMTSDVTAGFNLFLNF"
     gene            2039..2566
                     /locus_tag="ACHOQV_004851"
     CDS             2039..2566
                     /locus_tag="ACHOQV_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFP9633093.1"
                     /translation="MRNGKYDVLSPLYSGEPVNEAEVLGAVVWLWMHSPLHRDAPLHT
                     LPDLLLPVIKNRQYVVATEQGRPVFFMSQAWLSLDAEARYLTQPAILMPQSDWNSGDR
                     MWVCDWVAPFGHTPDMSRLVRQDIFPRLCWRSLYHRGQQTGLRVMNFRGVQVSREEAR
                     QWRVQHPLAVDVPER"
     gene            2582..>4307
                     /locus_tag="ACHOQV_004852"
     CDS             2582..>4307
                     /locus_tag="ACHOQV_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFP9633094.1"
                     /translation="MNRNCYRIIFNKARGMLMVVADIARSGRAGASPSSRTGYPRRQR
                     ICRVTPLAFSLWLASGMVHSVSAAGIVADHGAPGHQQPTIMQTASGIPQVNIQTPSAG
                     GVSHNTYSQFDVGNQGVILNNAHNNVQTQLGGMVAGNPWLAKGEARIILNEVNSRNPS
                     QLNGFVEVAGKKAQVVIANPAGISCDGCGFINANRATLTTGQPQMKNGSLTGFRVEGG
                     EIQVTGKGMDASRTDYTDIIARSVKINAGVWAQDLKVTTGRNNVDITHGQTEKKAADA
                     SSQPQVALDVSSLGGMYAGKIRLVGTETGVGVRNAGHIGAQAGAVTLTADGRIENSGS
                     ISAKTDVHLETTRELHNSGSVYAGQDTQIQSDGAFTQTGSVASRRDTRIQAARVTGSE
                     RSLLAAGVKDDGRLAAAGNLTVSTTGELAAHGQVLSGGDMQLKGQGLDLSNSRIQGQH
                     TGLDAGSGNLSTQNAQLSADTFAARTAGQFSNNGGTINAGTLQISAQSLSNHKGSLIQ
                     TGTGDFSLSLPGSVDNREGLLAANGAVSLDALSLDNRRGKVQAAQSGSLQVKTTGAVD
                     NLQGSLIAS"
BASE COUNT          980 a         1048 c         1289 g          990 t
ORIGIN      
        1 atatatattt ctaaatattc cagtttaaca acatatactg gaataaagat ctgcaagcat
       61 ctgcaattac gatcgataca cctgttatat tgatattttt cagatcatta ttctataatg
      121 agcaaaatta aaccagcctg atttggctga ctggttactc agcattctgg taccggttga
      181 tacgggaaat tccggaaggg cggtagtgtg ttattgccaa aatggcggca gacatcgtca
      241 ggaggctgac aggtgattgt tgacacagca tgcataaaag gtttttcgtt tggctggaaa
      301 attatgtcac agaggcgtgg tatctgaaga gcaacgcgta ttccggcgct gtcagttcat
      361 tttatcactg acactgatac ctgctttatt attgccttca tatcttcagg ctgctgatac
      421 ccggcataat ggaaatgacc catttatcat tcagcagcag cgtcaggaag cccttgagca
      481 acaactgacg ccttcagccc ctgatgttcg cctttctgca cctggctctt ttgcccataa
      541 gattaatttt cctgttgaaa cgccctgttt tcagattaaa cagacggaac tggagggggc
      601 tgatgcgtta ccacactggt tgcctttaca aaaaatcgcc aacggggcgg tcgggcattg
      661 cctgggggcg aaaggaatta atctgctgat gagtacattg cagaaccgtc tggtcgatca
      721 tggttatgtc accacccgtg ttctggcacc ttcgcaggat ttaaaaagcg gtatcctccg
      781 gctggttgtt attcccggtg ttgtgcgaca tgtgcgtctg acaccggaca gtgatgacta
      841 tattcagttg tattcctcat tcccggcaca cgaaggttct ctgctggatt tacgggacat
      901 tgagcagggg ctggagaata tgcaacgact gccgggtgtg caggctgata tggaaattgt
      961 gcccggtgag cagcctgggg aaagtgatat tcttattacc cgtaaacagg ataaatactg
     1021 gcgactgggg ttttcgcttg atgattcagg tacccgcagc acagggcgtt atctcggtgg
     1081 tataacattt tctctggata atcctttttc cctgagtgac ctgctgtatg tttctgccac
     1141 ccataatttt ccacattacg ggggtaaggg cagtaaaaac tacacggccc attactctgt
     1201 gcctttcggt tactggcagt tttccgtcac cgccagtgat tacgattacc atcagacggt
     1261 agctggtgcg gttgaggatt accagtacag tggcgagagt cagaatctgg gcatgcagct
     1321 gagccgggta ctgcaccgta acgggacgca gaaaacggtg ctgacttacg atgttcaggc
     1381 ccgcaggtcg cgtaactaca ttaatgatac ggaaatccag gtacagcgcc ggcagacggc
     1441 ggcctggcga cttggacttc agcaccgcca ttatatctcg caggccacac tggatgccgg
     1501 cgtcagctgg caacggggaa cccgctggtc tggcgcacag ccggcaccgg aagaaacgtt
     1561 cggcgaggca acggcgctca gtaagatcct gcatataaat gcatccttta atctgccctt
     1621 tacactggca gagcagccct tcagttattc cttccggtat cagcgccagc tgagcaatac
     1681 cccgctgact ccacaggaac agtttgccat cggtaaccgc tggacggttc gtggttttga
     1741 cggggagaga acgctgaatg ccagtaacgg ctggtatatc cgcaatgacc tggcctggcg
     1801 tacgccactg ccggagcagg cgttatatct gggaatggat tatggcgagg tgggcggagc
     1861 cggcagtgaa tacctggtgg gacgtcatct ggccggtggt gttgtggggc tgagggggcg
     1921 actgctcagt acggattatg acgttttcat cggtcgtccg ttttcaaaac cggcgggttt
     1981 tatgacgagt gatgtgacag ccggttttaa tctgttcctg aatttctgag aaacgttcat
     2041 gcgtaacggg aaatatgacg ttctgagccc cctgtattcc ggtgagccgg tcaatgaagc
     2101 ggaagtgctg ggagcggtcg tgtggttgtg gatgcactct cccctgcatc gtgatgcacc
     2161 actgcatacg ctgccggacc tgctgctgcc ggtgattaag aaccggcagt atgttgtggc
     2221 aacagagcag gggcgacctg ttttcttcat gagccaggcc tggctcagcc tggatgcaga
     2281 ggcgcgttat cttacgcagc cggcaattct catgcctcag tcggactgga acagcggcga
     2341 ccggatgtgg gtctgtgact gggttgcccc tttcgggcat accccggata tgagtcgtct
     2401 tgtccggcag gatatttttc cccgtctgtg ctggcgttcg ctgtaccacc gtgggcagca
     2461 gaccgggtta cgggtgatga atttcagagg tgtgcaggtc agtcgtgaag aagcgcgcca
     2521 gtggcgtgtg cagcacccgc ttgccgttga tgtgccggag agataagtca ggtttatcag
     2581 aatgaacaga aattgttacc gcatcatatt taataaagcc cggggaatgc tgatggtggt
     2641 ggcagatatt gcccgttccg gtcgcgccgg tgcatcaccc tcctcccgaa caggatatcc
     2701 acgcaggcaa cgtatctgcc gtgtcacgcc gcttgcattc tccctgtggc tggcctcggg
     2761 gatggtgcat tctgtcagcg ctgcaggcat tgtcgcagac catggcgcac cggggcatca
     2821 gcagcccacg attatgcaga cggccagcgg cattccgcag gtgaatattc agacgccttc
     2881 tgccggcggg gtgtcacata acacatacag ccagttcgac gtcggtaacc agggtgtcat
     2941 tctgaataat gcccataaca atgtgcagac tcagctgggt ggcatggtgg ccgggaaccc
     3001 gtggctggcc aagggagagg ccagaatcat tcttaatgaa gtgaacagtc gcaatcccag
     3061 ccagttgaac ggttttgttg aggttgccgg gaaaaaagcg caggtggtga ttgcaaaccc
     3121 ggccggtatc agttgcgacg ggtgcggctt tattaatgcg aaccgggcca cgctgaccac
     3181 tgggcagccg cagatgaaaa acggcagtct gaccggattc cgtgttgaag gtggtgagat
     3241 tcaggtcacg ggcaagggga tggatgcctc gcgcacggat tataccgata tcattgcccg
     3301 gtcagtgaag attaatgcgg gtgtctgggc gcaggacctg aaagtgacca ccggacgtaa
     3361 caatgtggat attacgcacg ggcagactga gaaaaaagcc gctgatgcct cttcgcagcc
     3421 acaggtggcg ctggatgtgt ccagtcttgg tggaatgtat gccggaaaaa ttcgccttgt
     3481 cggtacggaa acaggtgtgg gcgtgcgtaa cgccggtcat attggtgcgc aggccggggc
     3541 ggttaccctg acggctgacg gacgtattga aaacagtggc agtatcagtg cgaaaacgga
     3601 tgtgcatctg gagacaaccc gggaactgca taacagtggt tctgtttatg cagggcagga
     3661 tacgcagata cagagcgatg gtgcattcac ccaaacgggc tctgtggcat cacggcgtga
     3721 tacccgtatt caggctgcgc gcgtgacagg cagtgagcgg agtctgctgg ccgccggggt
     3781 gaaagatgac ggacgtctgg cggcagcggg caacctgact gtcagcacca ccggcgaact
     3841 ggcggcacac ggacaggttc tgtccggtgg ggacatgcaa ctgaagggac aggggctgga
     3901 tttaagcaac agccggatac agggacaaca taccgggctg gatgcaggca gcggaaatct
     3961 gtccacacag aatgcgcaac tgagcgccga tacctttgct gcccggactg ccgggcagtt
     4021 cagcaataat ggcggcacga taaatgccgg cacactgcag atatcggcac agagcctgtc
     4081 aaatcataaa ggcagcctga ttcagacggg aacgggggat ttttcactga gtctgccggg
     4141 aagcgtggat aaccgggaag ggctgcttgc ggcaaatggc gcggtgagcc tggatgcact
     4201 gagccttgat aatcgcaggg ggaaagtgca ggcggcgcag tcaggcagtc tgcaggtgaa
     4261 gacaaccggc gccgtggata acctgcaggg aagtctgatt gccagcc
//
LOCUS       DAZKGF010000275         4284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZKGF010000275 DAZKGF010000000
VERSION     DAZKGF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.275"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1245
                     /gene="frc"
                     /locus_tag="ACHOQV_004853"
     CDS             <1..1245
                     /gene="frc"
                     /locus_tag="ACHOQV_004853"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFP9633095.1"
                     /translation="TPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTR
                     HQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDH
                     MGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLV
                     SAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDK
                     LGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWE
                     NTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCA
                     PVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTA
                     AVLQELGYSDDEIAAMKQNHAI"
     gene            1299..2993
                     /gene="oxc"
                     /locus_tag="ACHOQV_004854"
     CDS             1299..2993
                     /gene="oxc"
                     /locus_tag="ACHOQV_004854"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFP9633096.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNGIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVASAISLLAKAERPLVIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            3063..4007
                     /gene="yfdV"
                     /locus_tag="ACHOQV_004855"
     CDS             3063..4007
                     /gene="yfdV"
                     /locus_tag="ACHOQV_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFP9633097.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            4081..>4284
                     /locus_tag="ACHOQV_004856"
     CDS             4081..>4284
                     /locus_tag="ACHOQV_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HFP9633098.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFIN"
BASE COUNT         1100 a          929 c         1052 g         1203 t
ORIGIN      
        1 actccacttc aaggaattaa agttctcgat ttcactggtg tgcaatctgg cccatcttgt
       61 actcagatgc tggcctggtt tggcgctgac gtcattaaaa ttgaacgtcc cggcgttggt
      121 gacgtaacgc gccaccagct gcgagatatt cctgatatcg atgcgcttta cttcaccatg
      181 cttaacagta acaaacgttc tattgaatta aataccaaaa cagcggaagg caaagaggta
      241 atggaaaagc tgatccgcga agctgatatc ttagtcgaga actttcatcc aggggccatt
      301 gatcatatgg gcttcacctg ggagcatatt caagaaatca atccacgtct gatttttggt
      361 tcgatcaaag ggtttgatga gtgttcgcct tatgtgaatg taaaagccta tgaaaacgtt
      421 gctcaggcag cgggtggcgc ggcatctact acaggttttt gggacggtcc gccgctggta
      481 agcgctgcag cgttgggtga cagcaacacc ggaatgcatt tgctgatcgg tttacttgct
      541 gctttgctgc atcgcgaaaa aacggggcgt gggcaacgag tcaccatgtc aatgcaggat
      601 gccgtgttga acctttgccg cgtgaaatta cgcgaccagc agcgtctcga taagttgggg
      661 tatctggaag aatacccgca gtatccgaat ggcacatttg gtgatgcagt tccccgcggt
      721 ggtaatgcgg gtggtggcgg tcagcctggc tggatcctga aatgtaaagg ctgggaaacc
      781 gatcctaacg cctatattta tttcactatt caggagcaaa actgggaaaa cacctgtaaa
      841 gccatcggca aaccagaatg gattaccgat ccggcataca gtacagccca tgcccgacag
      901 ccacatattt tcgatatttt tgctgaaatc gaaaaataca ctgtcactat tgataaacat
      961 gaagcggtgg cctatttgac tcagtttgat attccttgtg caccggtttt aagtatgaaa
     1021 gaaatttcac ttgatccctc tttgcgccaa agtggtagtg ttgtcgaagt ggaacaaccg
     1081 ttgcgtggaa aatatctgac cgttggttgt ccaatgaaat tctctgcctt tacgccggat
     1141 attaaagctg cgccgctatt aggtgagcat accgctgctg tattgcagga gctgggttat
     1201 agcgacgatg aaattgctgc aatgaagcaa aaccacgcca tctgatatta acgggggctt
     1261 ctggctccca atttataaaa atttcgaggt tattaatcat gtcagatcaa cttcaaatga
     1321 cagatggtat gcatatcatc gttgaagcat taaaacagaa tggtattgac actatttatg
     1381 gtgttgtagg tattcctgtg acggatatgg cgcgccatgc ccaggcggaa ggcattcgtt
     1441 atattggttt tcgtcatgag cagtcggcag gctatgccgc tgcggcaagc ggttttctta
     1501 cccaaaaacc ggggatctgc ctgacagttt ctgcgccagg attcctcaat ggtttgaccg
     1561 cattggccaa cgcaacggta aatggttttc cgatgatcat gattagcggc tccagcgacc
     1621 gcgcgatcgt cgacctacag caaggtgatt atgaagagct ggaccaaatg aatgcggcaa
     1681 aaccgtatgc caaagcagca ttccgcgtta atcagccgca ggatcttggc attgcattgg
     1741 cacgcgctat ccgggtctca gtatcgggtc gccctggcgg agtttatctt gatttgccag
     1801 caaatgtcct ggccgcgacg atggaaaaag acgaagcgtt aaccacgatt gtaaaagttg
     1861 aaaatccgtc gccagcatta ttgccatgcc cgaagtcagt cgctagcgca atttcgctat
     1921 tagcaaaagc tgaacgacca ttagttatcc ttggcaaagg cgcggcgtat tcacaagcgg
     1981 atgaacagct tcgtgaattt attgaaagtg cccagattcc attcctgcca atgtctatgg
     2041 cgaaagggat cctcgaagat acgcatccac tttctgcggc ggctgcgcgt tcgtttgccc
     2101 tggcaaatgc tgacgttgtc atgcttgttg gtgcacgact gaattggtta ctggcacacg
     2161 gtaaaaaagg atgggcggca gatacacagt ttattcaact ggatattgaa ccgcaggaaa
     2221 ttgacagcaa ccgccccatt gctgtgccag tcgttggcga tattgcatcc agtatgcaag
     2281 gtatgctggc agaactgaaa caaaacacat ttacgactcc actggtatgg cgcgatattt
     2341 taaatatcca caagcagcaa aatgcacaaa aaatgcatga aaagttaagc acagatactc
     2401 aaccattaaa ttactttaat gcattaagtg ctgtgcgcga cgtattgcgc gagaaccagg
     2461 atatttattt agttaatgaa ggggcaaata ccctggataa tgcacgaaat attattgata
     2521 tgtataaacc acgtcgtcgt ctggattgtg gcacctgggg tgtcatgggc atcggtatgg
     2581 gctatgccat cggtgctagc gtgacctctg gttctccggt tgttgccatt gaaggtgata
     2641 gtgcttttgg tttcagtggg atggaaattg aaacgatttg tcgatataac ctgccggtga
     2701 cgatcgttat ttttaataat ggcggcatct acagaggaga cggtgttgat ctcagcggcg
     2761 ctggtgcacc atcaccaacg gatctgctgc accatgcaag gtatgacaaa ttaatggatg
     2821 cgtttcgtgg cgttggctat aacgtcacta cgacagatga acttcgtcat gctttaacca
     2881 ccggtattca gtcgcgcaaa ccgaccatta ttaatgtggt catcgaccct gcagcaggaa
     2941 ctgaaagtgg ccatattacc aaacttaacc caaaacaagt cgctggtaat taaatactga
     3001 catcgtctcc tcttgccaac tcattatgcg atggtgaggg gacccttatc ggaaatacta
     3061 ttatgctaac attttttatt ggcgatttat tgcctattat cgtaatcatg ctgttgggtt
     3121 attttagcgg cagacgagaa actttttcag aagatcaagc ccgggcattt aataaactgg
     3181 tattaaacta cgctcttcct gcagctctat ttgtatctat tacccgggca aacagggaaa
     3241 tgatttttgc ggacactcgt ctgacccttg tatcacttgt ggttattgtc ggatgtttct
     3301 ttttctcctg gttcggttgc tacaaatttt ttaaacgtac tcatgcagaa gcagcagtat
     3361 gtgcattaat tgcaggttca cctaccattg gattcctggg atttgcagtt ctcgatccta
     3421 tttatggtga ttccgtatca acaggtttag tggtagcaat tatttctatt attgttaatg
     3481 caattactat tcctattggt ctgtatttgc tgaatccttc ctcaggagcg gatggtaaga
     3541 agaatagtaa tctgagcgca ttaatttctg cggcaaagga gccagtagta tgggcacctg
     3601 ttctggcaac gatcctggtg ttggttgggg taaaaattcc ggcagcatgg gacccaacct
     3661 ttaatctgat tgcgaaggct aactcagggg tagcggtatt cgctgcgggg ttgactctgg
     3721 ctgcacataa attcgagttc agtgccgaaa ttgcttataa caccttcctg aagctgattc
     3781 tgatgccact ggcgctgctt ctcgttggta tggcatgtca tttgaacagc gaacatttgc
     3841 agatgatggt actggcaggc gccttaccgc cggcattctc cggaatcatt attgccagcc
     3901 ggtttaatgt ctacacccgc actggtacag cgtcattggc tgtgagcgta ctgggttttg
     3961 tcgtcacggc tcctttgtgg atttatgtca gtcgactggt ttcataatta atcatgttat
     4021 tgattttaca taaatgccga agtgtctttt caccttcggc ttttttcaag gcataagcaa
     4081 atgacaaata atgaatgcaa agggccgttt gaaggcttat tagttatcga tatgacgcat
     4141 gtccttaatg ggcctttcgg aactcaactt ctttgtaata tgggcgcaag ggtaattaaa
     4201 gttgagccgc cgggtcatgg tgatgatacc cgcacatttg gtccctatgt ggatggacag
     4261 tcactctatt acagttttat taat
//
LOCUS       DAZKGF010000276         4244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZKGF010000276 DAZKGF010000000
VERSION     DAZKGF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.276"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1178
                     /gene="ybaT"
                     /locus_tag="ACHOQV_004857"
     CDS             <1..1178
                     /gene="ybaT"
                     /locus_tag="ACHOQV_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9633099.1"
                     /translation="LLMEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRR
                     GLGNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAV
                     MTLFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCI
                     GITFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALEL
                     EKYADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPK
                     LMNKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHD
                     IHASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            1209..1306
                     /locus_tag="ACHOQV_004858"
                     /pseudo
     CDS             1209..1306
                     /locus_tag="ACHOQV_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1303..1710
                     /gene="cueR"
                     /locus_tag="ACHOQV_004859"
     CDS             1303..1710
                     /gene="cueR"
                     /locus_tag="ACHOQV_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFP9633100.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(1711..2441)
                     /locus_tag="ACHOQV_004860"
                     /pseudo
     CDS             complement(1711..2441)
                     /locus_tag="ACHOQV_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2513..2719)
                     /locus_tag="ACHOQV_004861"
     CDS             complement(2513..2719)
                     /locus_tag="ACHOQV_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633101.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGING"
     gene            complement(2716..3138)
                     /locus_tag="ACHOQV_004862"
     CDS             complement(2716..3138)
                     /locus_tag="ACHOQV_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633102.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNADATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(3224..4240)
                     /locus_tag="ACHOQV_004863"
     CDS             complement(3224..4240)
                     /locus_tag="ACHOQV_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP9633103.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     TQTPARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDKQMQSLAQKSVQAHEQIES
                     LRQDSAQTQQQLSNTQKRVADNSQQINTLNNHYSSLKNEVEDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
BASE COUNT          944 a         1025 c         1111 g         1164 t
ORIGIN      
        1 cgttgctaat ggaagcctcg acctgggtcg cctttgcttt tggcggtatt gtggcgatgt
       61 tttccggtta tgcctatgcg cgcctggggg cgagctatcc cagcaatggc ggtattatcg
      121 acttctttcg tcgcggatta ggcaacggcg tcttttcgct ggcactctcg ttattgtacc
      181 tgttgacgct ggcggtgagc atcgccatgg tcgcccgtgc ttttggcgct tatgccgtgc
      241 agtttttgca tgaaggcagt caggaggagc accttatatt gctctacgcg ttggggatca
      301 ttgcggtgat gacgcttttc aactccttaa gcaaccatgc ggtagggcgg ctggaagtga
      361 tcctcgtcgg cattaaaatg atgatcctgt tattgctgat cattgccggt gtctggtcgc
      421 tgcaaccggc gcatatttcc gtctctgcgc cccccagctc cggtgcgttc ttctcctgta
      481 tcgggataac cttccttgcc tatgcgggct ttggcatgat ggcgaacgcg gcggataaag
      541 tgaaagatcc gcaggtcatt atgccacggg cgtttctggt ggcgattggc gttaccacgt
      601 tgctttatat ctcgctggcg ctggttttgc ttagcgatgt atcggcatta gagttagaaa
      661 aatatgccga taccgccgta gcgcaggctg cttctccgct gctcgggcat gtgggttatg
      721 tgatcgtcgt catcggcgct ttactggcga cggcttcagc cataaacgcg aacctgttcg
      781 ccgtgtttaa catcatggac aacatgggca gcgaacgcga actgccgaag ctaatgaata
      841 aatccctgtg gcggcagagt acctggggca acatcattgt cgtggtgctg attatgctga
      901 tgacggcggc gctgaattta ggctcactcg ccagcgttgc cagcgccacc tttttgatct
      961 gctatctggc ggtgtttgtg gtggcgatcc gcctgcgtca tgatattcac gcctcgttgc
     1021 cgattcttat cgttggtacg ttggttatgt tgttggtgat cgttggcttt atctacagtc
     1081 tctggtccca gggcagccgt gcgctgatat ggattattgg cgcactctta ctcagcctta
     1141 ttgtggcaat ggtcatgaag cgcaataaaa ctgtataaca tctctctgtg cgcagtacct
     1201 cctgtattat tgtggtggcg gtcgatattc gcactggcaa aaaaacgtgc ttgaatatct
     1261 gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa cgatgaacat tagcgatgta
     1321 gcaaaaatta ccggcctgac cagcaaagcc attcgcttct atgaagagaa ggggctggtg
     1381 acgccgccga tgcgcagcga aaacggttat cgcacctaca cgcagcagca tctcaacgaa
     1441 ctgaccttac tgcgccaggc acgccaggtg ggctttaacc tggaggagag cggcgaactg
     1501 gtgaatctgt ttaacgatcc gcagcgtcac agcgccgacg tcaaacgtcg tacgctggag
     1561 aaagtggcgg agatcgaaca gcatatcgaa gaactgcaat ccatgcgtaa ccagctgctg
     1621 gcgctggcga atgcctgccc cggtgatgac agcgccgact gcccgattat cgaaaatctc
     1681 tccggctgct gtcatcatcg ggcggggtga ttaaggcttg cgaaaatagt cgtccattat
     1741 tttcgtcagc tccggacgtt ccccgtcatc attgatggca aaatccagaa aaaaagcatc
     1801 tgcttgcggc gttcggtttt gcgccatcaa ccggaaggtg agggggcctg accaggtttc
     1861 ggatgggctg atgtgcttat cagggccgaa actctcccat gcgttgggcg gtaatgtttt
     1921 gcacgcggtg agttttggta tgtggtattg cagctctgtt ttgtgcagac attccccgcc
     1981 aggaaatacc agggtttgtt gctcatcatt aaaatgggtt gccgtgctga gcaccagtgt
     2041 tttgagcggt cgcccttcca gcgcggcggc agtagcctta tggtgtccgt cgagcagaac
     2101 gcagagatag gaatcaggga gataccaggc cagagcgcgg gcgattccag ttttcgagag
     2161 cagacaaaaa ccgggcggct ccctgttcac cctccgcaaa accgctgtag acaaattccg
     2221 cacaggtagg gcaaccagcc tcggaccaga aacgtattaa cgggacatcc ttgccagcag
     2281 agcgaatgga taagaccgtg cgagtgcgga tcgtaaatca cacattgacg cagaaatcct
     2341 ttcccggact tcaccgaaaa aatgacgccc gcagcggtat ttttcagcag acgtacttca
     2401 ggttttcgaa aggggaaaaa gcatggccac tccttagcca aaatttcagt tccctgaact
     2461 gtgccagaaa attacgctgg tgactattgc tctggaaatg gaaacagtgt ccttacccat
     2521 taatccccgt ttgtaccgaa agaatatgtt gcaaactttc taccataatg cgccccgact
     2581 gaaataagcc ttcctggatt tcattgttaa cattcacagc gtgaaccagg gaggctaata
     2641 acaccacttg caagaaggca aaaataaacg caccgataat cataatatgc gtcaggggga
     2701 ttttcctttg ctccttcatt ttaaataacc cacaaactgg ccttctttca ttaaggcgac
     2761 ttttcctgtg tgatgataaa caatgacatt atacagaccc gactgataaa ccgtggcatc
     2821 ggcattatct tcatctatgg cacataatga atcattgaga taagcaaaat tcttactact
     2881 cattctaaag gtcatgccaa atcctttata aatggctttt tttgccgcct gggcatgttg
     2941 tgcggcttct gcatttttaa atgcctgcgc ttcagaagca cttaaaatag ccgtctggcg
     3001 ctgctgttct gcgagataac aggtgtcatg gctgacgccc tgcgcctgac aatctgcgat
     3061 gcgggaagcg ggtgagctac agccagccag taatataacc gccagcaggg aaaaggattt
     3121 aatggagcta ttcatcattg ttaaccttaa ataatgtgcg aattaatgaa gaggtgaaac
     3181 ccggcaagct tccttgctct tatttatatt gaggattgaa atattagaac gaatatccta
     3241 cgccgacacc tgcgccgaaa tcagattgcg tatccgcaga aataccggct ttaagtaccg
     3301 tatgcgttcc ggcattaaaa gatgttccga cagacaccgc agattcaccg ttgaaggttc
     3361 ccgctcccgc tgacaccatc atcacgtcac cggttttaac ctgtggttgt gaggcgatag
     3421 cgatggcaga tgcagttccc gcattggctt ctttacggtt gtcctcaact tcgtttttca
     3481 gcgaactgta atggttattg agcgtgttaa tttgttggct gttatctgcg acccgttttt
     3541 gcgtgttgct taactgttgc tgcgtttgtg cggaatcttg tctcagactt tcaatttgct
     3601 catgtgcctg gacagacttc tgcgccagac tttgcatttg tttatcgtat tgagcccttt
     3661 ctttttgctg ttgtgctgcc acatcggttg tcagtgcatt cattttctgc ccttgttccg
     3721 tggtagtgcg ctgattagcc tcattttgag ccgccagtct ggcattaatc atctcctgat
     3781 aatgcgcagt attttgcgta tctccagtcg cggcgagcgt tttttgtgtg gctacgatct
     3841 gcgcctgttg ctctgtaatt tgcgtttgct gggcatcagt taccgcagac tgtcgggcgt
     3901 tgatcatgct ctgataacgg gcaggtgtct gtgtatcacc tgtagcggca acggtttttt
     3961 gtcctgcgtc aggatctgtc tggacagtgg gagtctgagt ggtatcaggt gtttcagaag
     4021 ggatgtagtg atgttcagtc aaataggtat acgcagcggg gctgttgtct gtgcgttcta
     4081 aaaagcgggt aatcttctgc tcaatctccg attgcgacat gccagagtta atggctgaaa
     4141 gctctgttgc cgccgcttca atggtatcac cagctaaaac aacagggctg gatgttgctg
     4201 aaattatgag tgccagtaaa gctacgttta cagttttcat tcat
//
LOCUS       DAZKGF010000277         4171 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZKGF010000277 DAZKGF010000000
VERSION     DAZKGF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.277"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..849
                     /gene="yeeO"
                     /locus_tag="ACHOQV_004864"
     CDS             <1..849
                     /gene="yeeO"
                     /locus_tag="ACHOQV_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP9633104.1"
                     /translation="GLFSWPGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISL
                     KSYFKPLNFSIIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIA
                     ALINLPGSALGSASTIITGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGV
                     MASFYTQDPQVKHVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWG
                     CRVVVGYVLGIMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEK
                     KPVVSE"
     gene            950..1025
                     /locus_tag="ACHOQV_004865"
     tRNA            950..1025
                     /locus_tag="ACHOQV_004865"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:983..985,aa:Asn,seq:gtt)
     gene            1338..2111
                     /locus_tag="ACHOQV_004866"
                     /pseudo
     CDS             1338..2111
                     /locus_tag="ACHOQV_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
     gene            2161..3096
                     /locus_tag="ACHOQV_004867"
     CDS             2161..3096
                     /locus_tag="ACHOQV_004867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HFP9633105.1"
                     /translation="MAGPADASQSVLKNNRSSLKGDFTESAELYLVLHDLSGHPINVS
                     EGLEFVQSGTNVSYVQISTIDYTQNLYGEYKATVTGGGEGIATLIPVLNGVHQAGLST
                     TIEFISAGTRPMNGTVSVNGANLPAASFPSQGFTGAYYQLNNDNFAPGKTAADYAFSS
                     TASWVDVDTSGKVTFKNVGDRNAVIITATPRSGGAIYQTQVRVKGWWVNHGNNLMQLS
                     QAENYCSNQVGNGYPLPRADLLSNGHMRREIGSLYGEWGDMGNYMKEADFYSMVYWSS
                     NSAGAGQQYIVSLETGTQNTYQTYEFFYGACYKQI"
     gene            complement(3358..>4171)
                     /locus_tag="ACHOQV_004868"
     CDS             complement(3358..>4171)
                     /locus_tag="ACHOQV_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFP9633106.1"
                     /translation="LTLVTMTMQDHATLKEIKDLAKNITQQYGTPDLCIEAMRKKNLK
                     TMIFISQLKAKTNFQVVGLTMKCYSPLCKSASRMDQNLRAPVPMTWAFMMREAGFPAV
                     RPKYELPLSKDVLDEVREEMRSLGSYIALNLEGSSQERTFSLSIAENLIEKIQSETDI
                     PIVIVHGPKGEEKARVLVDCYNNVFRLSLSPSIKRSAAIIKDAYIAITPDTSILHMAS
                     AYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNISLDEFAKALKSVLLRI"
BASE COUNT         1057 a          927 c          999 g         1188 t
ORIGIN      
        1 ggccttttct cctggccggg actgggattt gtcggggctg ggttgggttt aaccatttct
       61 cgttatattg gcgcagttgc aattttgtgg gtgctggcga ttggttttaa tcctgcgcta
      121 aggatttcgt taaagagcta ttttaaaccg ctgaatttta gcattatctg ggaagtcatg
      181 gggattggta ttccagcgag tgtcgaatca gtgttattta ccagtggtcg gttattaact
      241 caaatgttcg ttgccgggat ggggaccagc gttattgccg gaaattttat cgcgttctca
      301 attgcggctc ttatcaactt acccggaagt gcgctcggtt ctgcctctac gatcattaca
      361 ggccgaaggt tgggagtagg gcagatagcg caagcagaga ttcagttgcg gcatgtgttc
      421 tggctgtcca ctcttggatt aacggccatc gcctggctaa cggctccctt tgccggggtt
      481 atggcatcgt tttacaccca ggatccacag gttaaacacg tcgttgtgat tctgatttgg
      541 ctaaatgctt tatttatgcc tatttggtcc gcctcatggg tgctacccgc tggatttaag
      601 ggggctcgcg atgcacgtta cgccatgtgg gtttctatgt tgagcatgtg gggttgccgg
      661 gttgtagtcg gttatgtgct gggaatcatg cttggctggg gtgtggttgg tgtctggatg
      721 ggaatgtttg ccgactgggc tgtgcgggcc gtgctgtttt actggcgaat ggttactgga
      781 cgttggctgt ggaaataccc tcgacccgag ccgcaaaagt gtgaaaaaaa gccagttgtg
      841 tcggaataaa cgacaaaatg cagattattt cagcaaacga tttcaaattt aaaaaacagg
      901 ctttgacatt gtggatgggc atcgctaata ttcgcctcgt tctcacgatt cctctgtagt
      961 tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg
     1021 agccaaattc taaaaattcg ctttttagcg caatgtcact gaccttagtt gagcattgct
     1081 ttttaacgga tagcgggttt ttgacatctt aagcgccctc gacctttatg gttgagggcg
     1141 ttttgctatg aacgccatca ccattttccc ctcgattaca aaacttgagt aattcagtag
     1201 tctcccctct tgcaactcac acccaaaact gcttaacgaa aagttattaa ttttcaatca
     1261 tattgctatc aggatttaca ttttttcgct gtgctagaaa gggcgcattt atgttagctc
     1321 gttcagggaa ggtaagcatg gctacgaaga agagaagtgg agaagaaata aatgaccaac
     1381 aaatcttatg cgggatgggt attaaactac gccgcttaac tgcgggtatc tgcctggtaa
     1441 ctcaacttgt gttccctatg gctgcggcag cacaaggtgt ggtaaacgcc gcaacccaac
     1501 aaccagttcc tgcacaaatc gccattgcaa atgccaatac ggtgccctat acccttggag
     1561 cgctggaatc ggcccaaagc gttgccgaac gtttcggtat ttcgttggct gagttacgca
     1621 aactcaacca gtttcgtacg tttgctcgag gttttgataa tgtccgccag ggtgatgaac
     1681 tggatgtccc ggcacaagtt agtgaaaaaa aattaacccc gccgccgggt aatagcagtg
     1741 acaaccttga gcaacagata gccagtactt cacagcaaat cgggtctctg ctcgccgaag
     1801 atatgaacag cgagcaagcg gccaatatgg cgcgtggatg ggcctcttct caggcatctg
     1861 gcgcattgac agactggtta agccgcttcg gtaccgcaag aatcacgctg ggcgtggatg
     1921 aagattttag cctgaagaat tcccagttcg attttctcca tccatggtat gaaacgcctg
     1981 ataatctctt tttcagccag cataccctcc accgtactga cgaacgtacg cagattaaca
     2041 acggcttggg ttggcgtcat ttcactccca catggatgtc gggcatcaac ttctttttcg
     2101 accacgatct taagcgcaga aacgagctat ggtggggcgc aaacagtaga tataacgctg
     2161 gtggcaggcc cggcagacgc ctcgcagtcc gtccttaaaa acaaccggtc atcactgaaa
     2221 ggggacttta ccgaaagtgc tgaactatat cttgttttac acgatctatc aggtcatccg
     2281 atcaatgttt ctgaagggct ggaatttgtg caatcaggta ccaacgtgtc ctatgtgcaa
     2341 atcagcacaa ttgattacac tcaaaattta tacggtgagt ataaagccac tgttacaggc
     2401 ggcggagagg gtatcgcaac gctgatcccc gtgttgaatg gtgttcacca ggcgggtctg
     2461 agtaccacga tagaattcat tagcgcggga accagaccaa tgaacggtac agtatcagtg
     2521 aatggtgcaa acctacctgc agcttcattc ccttcgcagg gatttaccgg ggcgtattat
     2581 cagttgaata atgacaactt tgcaccagga aaaacggcgg ctgattatgc gttttcaagc
     2641 actgcctcct gggttgatgt tgatacttcc ggtaaggtga cattcaaaaa tgttggagat
     2701 agaaatgctg taataatcac ggcgacccca agatcaggtg gcgctattta ccaaacgcaa
     2761 gtacgggtga agggctggtg ggtgaatcac ggtaataacc tcatgcaatt aagccaggct
     2821 gaaaactact gcagcaacca ggtcggtaat ggctaccccc ttccccgcgc ggacctttta
     2881 agcaatgggc acatgcggcg cgaaataggg tcactgtatg gtgagtgggg agatatggga
     2941 aattacatga aggaagctga tttttattca atggtatatt ggtcttctaa ttccgcaggc
     3001 gcaggccaac aatacattgt atccctggaa acagggacgc aaaacactta tcaaacgtat
     3061 gaattttttt atggggcgtg ttataaacag atttaatttt tcgttataat ttcttacaga
     3121 gtgctccatt cggggcgctc ttttttacat tctctctaca gaacttgccc ttctctcaca
     3181 gaaaatgaat aaataaaaat gcgataccgc cagattcgcg ttcggtgtga ctatggttgt
     3241 atccggtgag tacagctttg atccaaaggc tggctttctg taatgattga ccagccacaa
     3301 agtattgatg acaccaccgt tcataacaga tcggcactac tatgcataag tactgcctca
     3361 tattctcaac aaaacacttt ttaatgcctt tgcgaattca tccagactta tattgtcaat
     3421 tttttgccca acgacgactg actccgagac atcggccatt gcgggccatc gcgttttgta
     3481 atcagcataa attgcaacaa cgggggtatt ataggcactt gccatatgta atattgaggt
     3541 gtcaggagtt attgcgatat aagcatcttt tatgattgct gccgaacgtt taatcgaggg
     3601 tgataaggat aaacggaaga cattattata acaatccact aacaccctgg ctttttcttc
     3661 gccttttggt ccatgaacga tcactattgg tatatctgtt tcactttgaa ttttttctat
     3721 tagattttct gcaatcgata atgaaaatgt acgttcctgc gagctacctt ctaaattaag
     3781 cgcaatgtaa gatcctaacg atcgcatttc ctcgcgcacc tcatcgagta catccttact
     3841 tagtggcaat tcatattttg gcctgactgc tggaaaaccc gcctcacgca tcataaatgc
     3901 ccatgtcatg ggtacaggag cccggagatt ctgatccatc cgggacgcgc tcttacacaa
     3961 aggggaatag cacttcatgg ttaaaccaac aacctgaaaa ttcgtttttg ctttcaactg
     4021 actgataaat atcatcgttt tcaggttctt tttacgcatc gcctctatac aaagatccgg
     4081 cgtaccgtat tgctgtgtta tgttcttcgc tagatctttt atttctttta atgttgcgtg
     4141 atcctgcata gtcattgtaa ctaatgttaa t
//
LOCUS       DAZKGF010000278         4166 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZKGF010000278 DAZKGF010000000
VERSION     DAZKGF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.278"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            98..766
                     /gene="ykgH"
                     /locus_tag="ACHOQV_004869"
     CDS             98..766
                     /gene="ykgH"
                     /locus_tag="ACHOQV_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFP9633107.1"
                     /translation="MNEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTRPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKSKYPAAICLILGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(1007..1702)
                     /locus_tag="ACHOQV_004870"
     CDS             complement(1007..1702)
                     /locus_tag="ACHOQV_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP9633108.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERFTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1695..3122)
                     /locus_tag="ACHOQV_004871"
     CDS             complement(1695..3122)
                     /locus_tag="ACHOQV_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP9633109.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3133..3852)
                     /locus_tag="ACHOQV_004872"
     CDS             complement(3133..3852)
                     /locus_tag="ACHOQV_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP9633110.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGLKVKVMHIAEVLMSR"
BASE COUNT         1103 a         1069 c          865 g         1129 t
ORIGIN      
        1 tcatgatgct ggatatttgc tgatatttaa cccgaacatt cctgatacag actattaatt
       61 catttatctg cggcagattt tattttctga atatattatg aatgaacaaa tcaaacagga
      121 tatcgatctg attgagattt tattttatct gaagaaaaag attcgtgtta tcctttttat
      181 tatagctatc tgtatggcaa tggtgctgtt gtttctgtat atcaataaag ataatataaa
      241 agtgagttac agtctaaaaa taaaccagac aacaccaggc atacttgtta gctgtgatag
      301 caataataat tttgcctgtc agaccacaat gactgaagat gttattcagc gaattactac
      361 attttttcag accagaccag atgtcaagaa cagagaaata aagctggaat ggtcaggaga
      421 taagagagat ttaccaactg ctgaagcgga aatatctcgc gtgcaggcgt ctattatcaa
      481 atggtatgcg tcagaatacc ataatggcag gcaagttctt gatgagatac aaacgccttc
      541 agcaattaac agtgagcttt atacaaaaat gatatacctg accaggaact ggtcattgta
      601 tccgaacggt gatggctgtg taaccataag ctcaccggaa ataaaaagta aataccctgc
      661 tgccatttgt ctgattctgg gattttttct aagcattgtg atttctgtaa tgttttgcct
      721 tgtcaaaaaa atggtagatg aataccaaca aaactctggg cagtaataaa cagcattctg
      781 acagagagtg cctgtataaa actatagtga caatgaccgg gaagggagcc cgtcttcagg
      841 aatggattcc tgtctttttc actgtaaata tcctggcatt attaatgtca ggatatttac
      901 agaagagcag cataaccatg acggaacgga gttaagtcag gttgtcatta gcgctattcg
      961 caacctcgtg ccgccttcag gtttatgtcc agacttcata tctctctcag caatcctcaa
     1021 taatcagata caccgctttc accggaccat gaaccccgac gactttgata agctcaatgt
     1081 ccgccgttga actgggacca ctgatgatgt taatacatga cggcattcgt tcaccggcct
     1141 gcgctttctg atgcaatttt tctgcgagtt gcgctacacg cggcaggatt gtgcttttac
     1201 gcaggataaa aagagaagat tccgggagca ggctcaatga acgcccgcgc ccggcggcgg
     1261 aaaaaagaac cacgcctccc gattcggtta aaccatattc agcatatacg acgcccactt
     1321 tagcctgctc tgcttgtgat atgttctcaa tacctttcgc cggatcccaa acaacggcat
     1381 tgcattcctg ctgcaaacgt tcgctaatcc ccaattcctc caacctcgtg tcaccactaa
     1441 tcacgaccga ctgatctccc agctctttac acagacgtat tgcagcttct gccgccttcg
     1501 cctcgctggt cagttcacag cgcgtcaaca taacatcgct ggcaaactgg ataaacgcgt
     1561 cacagcgctg ctgttgggta agttgggtaa accgctcgtt agcatagttg ttaagcggcg
     1621 catcttctgc ttgcggttca agtcgcagcg ggcgacccag tgcctgagca acgttattca
     1681 aaaattcgct ccgattatcc attctttttc tcctgcgcct gatgtttctt aaaccaacta
     1741 cggaaactct ctccgtcagc ttcaggaaga tcgcgtgctt ccatccagtc gctaatcgcg
     1801 ccaaatttga gtggtgtttt gccgccattg ataaaccagc ttgccgcatg agcaccagcc
     1861 atcatcccaa ctttccacaa tcctggatga ctattggcat aagcgaacat ttttatcgcc
     1921 cgttgctctg cttttgcggt gatccctttt tcagccatca cccgacgatg acgcaaaatc
     1981 agttttgaca gcggaatacg caccggacac acgctgtcac aagcggtgca taaagagcag
     2041 gcgtagggta aatctttaaa atctttatag ccgccaagta acggggaaat caccgcacca
     2101 attggccctg gataaataga gccatatcca tgaccgccaa tatggcgata tgccggacaa
     2161 gtattcatac aagccccgca acgaatacag cgcagcacat cccgaaattc agaggccagc
     2221 acctcagaac gcccgttatc gacaataacc agatgaaact cttcaggacc atcaacgtgc
     2281 cccgcttcgc gcggtcctgt cagccaggtg ttgtatcccg tcaaacgtgc accaacggca
     2341 ctgcgcgcca gcatggtgat caatacatct acctctgcaa acgtgggagc aatacgctcc
     2401 attcccatca ctgcaatatg cgttttaggc agcgtggtac acattcgcgc attaccttca
     2461 ttggtcacca ggcataccga accggtctct gccaccgcga aattacagcc ggtaatacct
     2521 atttcagcac tgaggaaatc ttcgcggatt ttttgccgaa tgaataaggt catcgcttca
     2581 ggcgtttccg gcccctcata gcccagacgt tcgtgtagca cccgacggat ctgatggcga
     2641 tctttatgaa ttgccgggac cacaacatga gagggcggat cttgatccag ctggagaatg
     2701 tattcaccca gatcggtttc aatcacctga atgccagcat cctgcaacac atgattgaca
     2761 ccaatctctt cggtcaccat cgatttagat ttcaccacct tccgggcatt tttgcgttgg
     2821 gcaacctgta aaatgtagcg ggtagcgtct tctttggttt ttgcaaaata gacgtgaccg
     2881 ccgttttgcg tcactttttc tgagagctgg tacagataag cgtcgagatt actcagaaca
     2941 tgatcacgta tctgggcggc ccgatcgcgc cactcctccc agtgccccaa ttcatcgacc
     3001 attttttgcc gatttgcccc gatacgctgc tgcgcgtttg ccaccgcttt gcgcatgatc
     3061 ggatcttcaa tttgctgacg gatgcgtgtc ttaaaatctg tattactggt tttgatcgac
     3121 atctttatat cctcagcggc tcatcaacac ttcagcaata tgcatcactt tgactttcag
     3181 cccttcccgt tgtaatcgtc cactgatgtt cagcaggcaa ctcacgtcag cacctattaa
     3241 atactcaggg cggacttcca tcaggtgcgc aaccttttct ttcaccatct cgccggatat
     3301 ttcggccatt ttgaccgaga acgtgccgcc aaatccgcag caggtatcct gttcagcaaa
     3361 ggtcaacagc tccagtccac gcacattttt cagcagcgta agtggttcgt ccttcacccc
     3421 cagcttacgg gccaggctac aagatgggtg atacaccgct ctcccttgca aactggcacc
     3481 tacatcgact acccctaatg tattaacaat aaaagaggtg agatcctgca tacgcgcggc
     3541 aaccttttcg gcacgtgatg cccattcagg ttcatccgcc agatacgtcg ggtaactttt
     3601 tacggcatag gtgcaagagc cagctggtga aataatggga tcgtcgttat cctccagtgc
     3661 ggcgatcaga tttttcatcc ctggaatcgc ttctttgata taaccactat tgatcgcagg
     3721 ctgaccgcag catccctgtt tctccgggaa atttacgcga cagccgagtt tttccagtag
     3781 cagcacggag tctcgtgcca ttcttgattt cagggcgtca ccaatacagg tgacaaagaa
     3841 attgacattc acaactaata ctccattact tcatgcccat ttatgcccga tggttccccc
     3901 cttccggggg atcaatcatc aataaatatg gcgttctttt ttaattggct agcaacatac
     3961 cacccctcat ttgtatgaca aatgataatc tttcatccaa acttcaaatg caataactaa
     4021 ttttatttct ttcggcatcg ctcacatttt atagggaaat cgacgatcat ttttggcata
     4081 agaaatccat ttaaaaaggc tacatccctt atacctattc agtgctactt attcaggggt
     4141 tattgagata tacactgatg gtgtac
//
LOCUS       DAZKGF010000279         4150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZKGF010000279 DAZKGF010000000
VERSION     DAZKGF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.279"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..293)
                     /locus_tag="ACHOQV_004873"
     CDS             complement(<1..293)
                     /locus_tag="ACHOQV_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633111.1"
                     /translation="MKKVNVVGYTELIFGAISEKTEIKSKKLKRRCNDLIEKETPSRI
                     FKFTNFLNCGVQHLFVLHRMKTNIPYKTNDDMKKSTGGIITGSKFIGLIIVDL"
     gene            complement(403..630)
                     /locus_tag="ACHOQV_004874"
     CDS             complement(403..630)
                     /locus_tag="ACHOQV_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9633112.1"
                     /translation="MEIENTPENIKRLRKKIGLTQKECAEMFGMTARSWRRKEEPTGT
                     VSGSKLSPVEFEYILLLAGEHPEYVLSKRDA"
     gene            complement(620..880)
                     /locus_tag="ACHOQV_004875"
     CDS             complement(620..880)
                     /locus_tag="ACHOQV_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633113.1"
                     /translation="MKMRKGDRQYYLNKEGDTFRLVKRVKTFSKSATLGKTKATVKTV
                     ADIIFREEEFGSIDFTQDGLREGDKEIISIMLKNIKRDENGN"
     gene            complement(1071..3332)
                     /locus_tag="ACHOQV_004876"
     CDS             complement(1071..3332)
                     /locus_tag="ACHOQV_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFP9633114.1"
                     /translation="MRLWIAEKPELGKLIAQVLGNPQYFDGYIVCNNDTVSWAIGHLL
                     KLIPPELKTPEYKSWSKNTLPLQLRPLQYIVIPKTEKQFNVLSGLITRADMIIHAGDP
                     DEEGQLLIEEILSYCGNKAPVKRVLINDLNKDAVRQAMRNLQDNRAFYGLYQKALARS
                     AGDYLFGLNMTRAYTIAAREKGHNEILSVGRVQTPVLGLVVRRYEENRDYKKYDYWCV
                     QAEFIDTDTIISPMLVPPEDAPVDDKGRIISEQYCDEAIRSCKGQQASVSLAESEIKS
                     RRPPLPFSLLDLQVKMNRQYGFTAEKVLEITQKLREEYRAITYNRSDCRYLSEEQFSQ
                     SPATLAMLSSFFPELGKIFSHIDSGRKSRAFNDKKISAHTAIIPTPTPTGLDQMNEDE
                     MRVYKAIVAQYLVQFMPDKTFDSAVVEFNINGFLFRQRAIRPVSPGWSILLADRDEET
                     LPDCKLFDAITSLTKGQQLVCSSVNSRKETSTPPPLYTEASLLEDLRRVARYVEDPRI
                     RQLLLDRDEGKEEERGGIGTPATRGHILNVLQVRGFIAVKNKKVVPTELGLSFIHALP
                     EVMTRPDMTALWHEQQSMIETGEISIDDFLDELEQFIFQQVSDVDISGLAVAEYQCTC
                     GGRYVRRRTDKGFFWGCSKYPDCRNTVPDRGGVPDFSRSEFERKVRCPVCGSGMKISP
                     KAYNCSNERCGFRLWGTQFGKELTLAQATDLLMKGHTREIKGLKKKDGAKFDAVLNLN
                     RDGSVVPTFKKKK"
     gene            complement(3373..3606)
                     /locus_tag="ACHOQV_004877"
     CDS             complement(3373..3606)
                     /locus_tag="ACHOQV_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFP9633115.1"
                     /translation="MENKYRKYPKSSKQEYLFQLRKCSKLSTLDKIIQRKAYTMNDID
                     FVEFLTAADHRLAEIVTGQLFDKIPKNVWKLIY"
     gene            complement(3656..4012)
                     /gene="ssb"
                     /locus_tag="ACHOQV_004878"
                     /pseudo
     CDS             complement(3656..4012)
                     /gene="ssb"
                     /locus_tag="ACHOQV_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(4024..>4150)
                     /locus_tag="ACHOQV_004879"
     CDS             complement(4024..>4150)
                     /locus_tag="ACHOQV_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9633116.1"
                     /translation="RAFLKGSLDLSIQEAEEDIDEREDIYRQNGAQPDYLSYLED"
BASE COUNT         1137 a          981 c          658 g         1374 t
ORIGIN      
        1 tccagatcaa caatgattag tcctataaat ttactacctg taattatccc tccggtagac
       61 ttcttcatat catcattagt tttatatggg atatttgttt tcattctgtg gagtacaaat
      121 aaatgttgta ctccacaatt taaaaagttt gtaaatttga atattctgct tggtgtttcc
      181 ttttctatca gatcattaca tcttctttta agttttttac tctttatttc tgttttttca
      241 gatattgcac caaaaattaa ttcagtatat ccaacaacat ttactttctt catttttaat
      301 ctcctgccgc tgtgcggctc tggtgattgg ggccatcccc tctcgatgta ttaataatag
      361 gtcataaacg acctatttgc aagttatttt tcttcttttt ttctaggcat cccgtttaga
      421 aaggacgtat tcaggatgct ccccagccag taacagaatg tattcaaact ctacaggtga
      481 aagttttgat ccgcttaccg ttccagtggg ttcttctttg cgccgccatg aacgggctgt
      541 catcccgaac atctctgcac attctttctg cgttaatcca attttctttc gcagacgctt
      601 gatattttct ggtgtatttt caatttccat tttcatctct ttttatgttc ttaagcataa
      661 tagaaattat ttctttatcg ccctctctta agccgtcttg agtaaaatca atagagccaa
      721 actcttcttc acgaaaaata atgtctgcca ctgtttttac cgttgctttt gttttcccta
      781 aagtcgcaga cttagaaaaa gtttttaccc gcttcaccag acgaaacgta tcaccttctt
      841 tattcagata atattgcctg tcaccttttc tcattttcat atctatatga ttctctctaa
      901 gagaagcctc aagcggagcc gaacgactcg tacactgtac gcatggtttc cctattgcaa
      961 cagatttaac atacttttcc atcttcaaac tcctgccgct gtgcggcttt ggtgacagag
     1021 aactatccct tctcgatgca aaggtaggtc ataatcgacc tatttgcaag ttattttttc
     1081 tttttaaatg tcggtacaac gcttccatca cggttaagat tcagcacggc atcaaactta
     1141 gcaccatctt ttttcttaag acctttaatt tcccgcgtat ggcctttcat caacaaatct
     1201 gtagcctgtg ctaaggtcag ttctttacca aactgagtac cccacaatcg gaatccacat
     1261 cgttcatttg aacagttata ggcttttggt gatatcttca tcccggaacc acataccggg
     1321 catctgacct ttctctcaaa ctcgctacgc gaaaaatcag gcacgccgcc tctgtctgga
     1381 acagtattcc gacaatcagg atacttactg catccccaaa aaaaaccttt gtcggttctc
     1441 cgacgaacat aacggccacc acacgtacac tgatactctg ctacagccag gccagaaata
     1501 tccacatcag acacctgttg aaaaataaac tgttcaagtt catcaagaaa atcgtcgata
     1561 ctgatttccc ctgtctcaat catagattgc tgctcatgcc atagagccgt catatctggt
     1621 cgcgtcatga cctccggtaa agcatgaata aacgataagc ctaactctgt aggaaccact
     1681 tttttattct tgacagcaat aaaaccgcga acctgaagta cgttaagaat atgccccctt
     1741 gttgccggtg ttccaattcc ccctcgttcc tcctctttac cttcgtctct gtccagtaaa
     1801 agttgtctaa tcctgggatc ttcgacataa cgggccacac gccgtagatc ctcaagcaat
     1861 gatgcttctg tatacaatgg tggcggtgtg gatgtctctt ttcgagaatt cacactactg
     1921 caaaccaatt gttgaccttt tgtaaggctg gttatggcat caaataactt acagtccggc
     1981 aaggtttctt cgtctctgtc tgctagtaat atactccagc caggggaaac aggccgtatc
     2041 gctcgttgcc ggaacagaaa tccattgata ttaaattcaa ctaccgcaga atcaaaagtt
     2101 ttatcaggca taaactgcac aagatactgt gccacaatcg ccttataaac cctcatttca
     2161 tcttcgttca tttgatccag cccggtaggc gttggtgtcg gaataattgc tgtatgagca
     2221 cttattttct tatcattaaa tgccctgctt tttcgcccac tatctatatg agaaaaaatt
     2281 tttcccagtt caggaaaaaa agaagaaagc attgctaatg tcgccggaga ctgagaaaac
     2341 tgttcttctg agagataacg gcaatccgaa cggttataag tgatagccct gtattcctcc
     2401 cttaattttt gcgttatctc caacactttt tcagcagtga atccatactg acgattcatt
     2461 ttcacctgaa gatcaagaag cgaaaatggt aatggtggtc tcctgctttt aatttcactc
     2521 tctgcaagag aaacactcgc ttgttgtccc ttacaactac gaattgcttc atcacaatat
     2581 tgctcgctga taattctacc tttatcgtcc acaggtgcat cttccggtgg aacaagcatc
     2641 ggtgaaatta ttgtatcagt atcaataaat tcggcctgaa cacaccaata atcatatttc
     2701 ttataatccc tgttttcttc ataccgccga acgaccagtc caagaactgg tgtctgaacc
     2761 cgtcctactg aaagtatttc attatgccct ttttctctgg ctgcaatagt gtatgctctg
     2821 gtcatattta aaccaaaaag ataatcacct gcactacggg caagagcttt ctgataaagc
     2881 ccgtaaaacg ctctgttatc ctgtaaatta cgcatcgcct gacgcactgc atctttatta
     2941 agatcattaa tcagaacccg tttaacggga gctttatttc cacaataaga aagtatctct
     3001 tcaataagta actgcccttc ctcatctgga tcccctgcat gaataatcat atctgcacga
     3061 gtaattaatc cagacaaaac attaaattgt ttctctgttt ttgggataac gatatattgc
     3121 aacggtcgta attgcagtgg taatgtattt ttagaccagc ttttatattc aggagttttc
     3181 aattcaggtg gtattagctt taacaaatga ccaatagccc aagaaaccgt atcattgttg
     3241 caaactatat acccatcaaa atactgtggg tttcccagaa cctgagcaat aagcttccca
     3301 agctctggtt tttcagctat ccagagtcgc ataagaatat ctccgcttga aaagtacgac
     3361 aggatgtatg atttaataaa tcagcttcca gacattttta ggaattttat caaataattg
     3421 tccagttacg atttcagcca accggtgatc tgcggctgtc aaaaactcta caaaatcaat
     3481 atcattcatt gtatatgcct tacgctgaat aattttatca agagtagaaa gctttgagca
     3541 cttcctgagc tgaaacaaat actcctgctt actacttttt gggtacttcc tgtatttgtt
     3601 ctccatttaa gttattcctc aaacggactc aatatcttca agactgtcct gtttttgatt
     3661 tagtattatt accaagcatt tgcatagttc ccttctgatc aacaacaatc tctgttgtat
     3721 aatgctcctc cccatcttta tctgtccatt ttctgtaacg taatgttccc tcaatataga
     3781 cctgcattcc tttacataaa aaatctctgg atatctctgc cagtttgtta taaataacaa
     3841 ctcgatgcca ttctgtattc tcttttattt caccagactg tttatctctc cacgtttcgc
     3901 ttgttgctat acttaaatta gcaacggtaa ctccattctg catacaccga atctcaggat
     3961 cttgtccaag attacctatt aatgtaactt tatttatacc tttagcagcc atttaatacc
     4021 ccactaatcc tcaagataac ttaaataatc tggttgtgcc ccgttctgtc tgtatatatc
     4081 ttccctttca tcaatatcct cctccgcttc ctgtatactt agatcaagag atccttttaa
     4141 aaatgcacga
//
LOCUS       DAZKGF010000280         4002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZKGF010000280 DAZKGF010000000
VERSION     DAZKGF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.280"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..480
                     /locus_tag="ACHOQV_004880"
     CDS             <1..480
                     /locus_tag="ACHOQV_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP9633117.1"
                     /translation="PETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDA
                     TANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRLLELELNNWIRGLVTEMTDP
                     GDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
     gene            496..1833
                     /gene="tssK"
                     /locus_tag="ACHOQV_004881"
     CDS             496..1833
                     /gene="tssK"
                     /locus_tag="ACHOQV_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP9633118.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSERSLVDIVLA
                     LPLLNANGGNLDDGSESERPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCKDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQNGVMWEGAL
                     HDARLREGADFWLSVRSSIPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            1830..2483
                     /gene="tssL"
                     /locus_tag="ACHOQV_004882"
     CDS             1830..2483
                     /gene="tssL"
                     /locus_tag="ACHOQV_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFP9633119.1"
                     /translation="MNKPVISRAEQIFYPGWLMVSQLRCGQPVEDGKALYRRACQLVT
                     QAREELAEAEFSQESSEIMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRAGRTMYWLSWVAGIVTLAGLWCVLSSVLADQVARLTGQG"
     gene            2486..>4002
                     /locus_tag="ACHOQV_004883"
     CDS             2486..>4002
                     /locus_tag="ACHOQV_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9633120.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWYCLSSRRDAPEKAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSQLLNDWQ
                     QRGVPQRYGLGLYQGMWLIPPVERATGEWIAPLPPRPVIQKVTQTPKMVRLNSLALFD
                     AGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPQRNQALSLKRAEAVR"
BASE COUNT          797 a         1026 c         1214 g          965 t
ORIGIN      
        1 ccggaaactc gggagtttga attcgctaat ctgggcttta ttccgctctc ttactacaag
       61 aaccgggatt atgcctgctt cttctccgct aactccaccc agaaaccggc actgtacgat
      121 acgccggatg caacggcgaa cagccgtatt aatgcccgtc ttccctatat cttcctgctg
      181 tcccgcattg cgcattacct gaagatcatt cagagggaga atatcggtac caccaaagat
      241 cgccgtctgc ttgaactgga actgaataac tggatccggg gtctggtgac ggagatgacc
      301 gatccgggtg atgaactgca ggcctcacat ccgctgcgtg acggaaaagt ggtggtggaa
      361 gacatcgaag acaacccggg atttttccgc gtgaaactct atgctgtacc acatttccag
      421 gtggaaggta tggacgtcag tctgtcactg gtttcgcaga tgccgaaggc aaaagcgtaa
      481 ggcaggcgtc gggaaatgaa gatttatcgt ccactgtggg aagacggggc ttttctgatg
      541 ccccagcagt ttcagcagca ggctgcctgg gatgttcatc tggcagacag cgttgcccga
      601 atggggctgg cgcatccctg gggcgtggtg gctgcagagt ttgatgactc tctgttaccg
      661 ctttcacggt tgaatgccac ccgtctgatt gttcgtttcc cggacggaac gcttattgat
      721 acggaacgtg cggataatct gcctccggtc tgtgatttat cgacggtttc tgagcgttcc
      781 ctggtggata ttgtgctggc gctaccgtta ctgaatgcta acggtggcaa ccttgatgat
      841 ggcagtgaga gcgagcgtcc gcgtcgctgg aaatcagaaa gggtgaatgt gcaggagctg
      901 gccgggcatg agcaaagcga agtggcggta ttgcggcata acctgactct gcgcatggct
      961 catcaggaaa atgctgcctg gctgacctgt cctgttaccc gtcttgttcg agatgcacag
     1021 ggacagtggt gcaaggaccc gcgttttatt ccccctctgc tgacgctgtc tgccagtcct
     1081 tcgctgatga ctgagcttgc ggagttgctt catcacctgc aggcgcgtcg ccagcgactg
     1141 atgtccatgc gtcgggagaa caatgcccgt cttgcggatt ttgcagtggc ggacgtgtcg
     1201 ttgttctggt tgctgaatgc cctgaacagt gcggagcctg ttctgaaaga gttgcttgac
     1261 atgccttacc gccacccgga actgttgtac agggagcttg cccggctggc aggctccctg
     1321 ctcacattct cgcttgagca taatgtggat gcggttccgg cataccgtca tgaaacacct
     1381 gaaaatgtgt ttccaccctt gctttctttg ctgaaccagc tgctggaggc aagcctgcct
     1441 tcccgtgtgg ttttcattga actgaaacaa aatggtgtga tgtgggaggg ggcattacat
     1501 gatgcgcgtt tgcgcgaagg ggcggatttc tggctttcag tgcgctcttc catacccggg
     1561 catgaactgc agacgaagtt tccgcagcta tgcaaagccg gaagccccga tgatgtgtct
     1621 gaggtggtga atgtggcgct gagcggggtc attatccgtc cggttacgca tgtaccggcg
     1681 gccattccgc tccggctgga aaaccagtat ttcgcgctgg atttaagtac ggatgcagcc
     1741 cgggcgatgc tggacgcggg gcgttgtact ttctacaccc cggcatcgct gggggatgtg
     1801 aaactggaac tttttgcggt actgcggaca tgaataaacc tgttatctcc cgggctgaac
     1861 agatttttta tcccggctgg ctgatggtca gccagctgcg ctgcggacaa ccggttgagg
     1921 atggtaaggc cctgtatcgc cgtgcctgtc agctggtgac gcaggcgcgt gaagagctgg
     1981 ctgaagcaga attcagtcag gaaagcagtg aaatcatgtt gtatgccttc tgcgccctgc
     2041 tggacgagag tgtgctgaac cgtgaaaaaa cagacgatgg ctggcacacc tggcagcagg
     2101 acccgctgca ggctcatttt tttggtacgc tcaatgccgg agaagagctc tgggagcgta
     2161 ttcgtgagca actgaaatta cctgcgccgg atgtggcggt actgacatgc ctctgccgta
     2221 cgcttcagct cggttttacc ggtcagtacc ggtcgcagga tgatgagcgt cgcgaagatg
     2281 taattcgcgc actgacagcg cgtgttcctg catttacttt cgcacaggat gcgccggtgg
     2341 tggtgcgtgc accgggttac cgtgctggca gaacgatgta ctggttgtca tgggtggcgg
     2401 gtattgtgac gctggctggt ctctggtgtg tgctttcttc tgtgctcgca gaccaggtgg
     2461 cacggcttac cgggcaggga taaccatgca cgtacgttcc cgtctctggc tgacaatact
     2521 gagtctgttc ctgacactgt ggctgatttc gggattcctg ccactgtctg cagtaaacca
     2581 gataatactg accctggttt cagttgtggc ggcgggactg gtgctgtggt accagtggta
     2641 ttgtctgtct tcccgtcgtg atgccccgga gaaggctggc gatgacggtc tgccgcctga
     2701 atattttcag ggacatgtgc tgcttgttgc cggaaactca gatgcgtggt tcgaacccgg
     2761 tcaggcatac cgggaaacag catcgggatg gtatcttcgt gtggatacgc cggagcagat
     2821 gagaatactg gcagagcggt tgtcgtacag ccgtccggcc ctgtgtggcc aggtcgtggt
     2881 catgctggga cttttaccgg agcagcatac ctcagatgaa gctttcctgt cgtggttgcg
     2941 gaactggcag cgtgcagtca tgcagtgccg gcagtggctg aataccatgc cgccggtgct
     3001 ggtgacattg atggtttcag ggccgtcacc acaaaacaca tccttaaata agcactctgc
     3061 acactggttt acactgacac cggacctgtc gggaatgcac atacgcctga cggaagccgg
     3121 agccattccg gcagaggaat ggctccggga gtgtgatgca gcgtccaggc tccggtgcgt
     3181 cagtgaactg ttatggctga atgccatgtt gtcctggtac aaccgggtta ctgatgccct
     3241 gacagatatt tgccggcagg agcactttta tttccggccg gttgccgttg gattctgtct
     3301 gacgtcggtt gcggcccggc ccggtaatct ctggcaacaa caactgacgt cactcactgt
     3361 actgccaccg cctgccggcg tgtcatcaca ggcactgccg ttgccggata ttctgctgtc
     3421 cggtattccc cgtcatcgtg gactcagtca ctggcacaga ctttgtctcc agacgggggc
     3481 gattgtcggc gtttttcttc tgctggctat gctggcgtct tttatgaata accagcgact
     3541 ggttcgcagc gtgggggaac acctgacggc ttggcaccgg atgcgcggat attcatctga
     3601 atcgaaaaaa actgcactgc aacgtcttca ggctgacagc cagttactga acgactggca
     3661 gcaacgtggt gtgccgcagc gttacggact gggattgtat cagggaatgt ggctgatacc
     3721 gccggttgag cgagcgactg gtgagtggat tgcaccactg cctccccgtc cggtaataca
     3781 gaaagttact cagacgccga aaatggttcg ccttaacagc ctggcgctgt ttgatgccgg
     3841 taaatggacg ctgaaaccgg gcgccacaaa atggctggtg aatgccctgg tggatattaa
     3901 ggcaaaagcg ggctggctga ttgtggtcag tggtcacacg gacaatacgg gcgacccgca
     3961 gcgcaaccag gccctgtcac tgaagcgcgc agaggccgtt cg
//
LOCUS       DAZKGF010000281         3981 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZKGF010000281 DAZKGF010000000
VERSION     DAZKGF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.281"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(110..760)
                     /locus_tag="ACHOQV_004884"
     CDS             complement(110..760)
                     /locus_tag="ACHOQV_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFP9633121.1"
                     /translation="MIRKSCIAACLSITLCSLSYAGGVPTVSIAELTQLVVNAQQQAQ
                     EALAQLNKAKEAIRQAKDQYDHYKSILQGNDKLGAFLNDPYINKLLPMKDWQDIYNQT
                     QDLTDLRKRYGITGYEPDVQKLFDKLLAQVDVYEKQYDATVTRVNHAEELRNKLNSVQ
                     TPKDREQLALRYQQEMLELENQRMQLENTRYLMEQKERIENKKNEEDFYNFMWGRS"
     gene            complement(829..3363)
                     /locus_tag="ACHOQV_004885"
     CDS             complement(829..3363)
                     /locus_tag="ACHOQV_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFP9633122.1"
                     /translation="MQDALFKNAFSPESNAKVPWLGHHVAPEICTLGTSHILSLIRFS
                     GVPHDTRDQAMLNKEFMYLARCFKALGKQEGNNLKLQTYTFKRRIHLTHQYNLELPVL
                     QDLVDCWVKPFRNGTFRQVGYSMALILKFRDLDDGINRMRELLSLCREMLGDFGVSVM
                     ELEERDGKLYSQIGRFYSFILNGEEKDIPVTDTRLGDSIIDSETGFGAYDYVENRPSY
                     GKRRFASTYDLRDYPESKSYPGMWDEVLDEQYDFCMVQSFLFEDRNRMKRRLKTQMSD
                     LSSTEGQSSETEELADSVQEVTQGKKVFGEYYASLIVFGDTPEEAVDNGARMQSLLMA
                     RDTGFVRSRSTNYVTWLTLFPGYADVIYSMPKSTENLACGFSLHATPCGKADGNPPGD
                     GTALMPVMTENGGMYFLNAHASPLGKNCRGEKYPGHKTTIAMTGAGKTTVESLELVFF
                     SRWNPMIFSLDYNNSLENVLRALGTRYFNIKPGEFTWIQPFQMEDSVELRQYLFDVLV
                     QCAGGADSTEESKIQMSIDAVLKHKNVESRSFSLLLQSIPDTGPGGLRERLSKWCRDD
                     GNGRRGQYYWILDSPRNDFDPTLFRRLGFDCTKILNMEFATRHPQAMEVLLNTFFFLK
                     RRMHENEPGSLLINSIAEFWAPLMFESTANSIMEILHAGRTRGEMVMMDTQVPEYVLN
                     NERGPSVIQQTITQEWMANEKATSDSYSKFSVTSKEFSKIEEFSAYSRKVLVKQGGAS
                     VVLKVDMQDKLKYWLPLLSSDYANIPVAKRVREALGTDDPAVWVAPFLDEMVAIGVRK
                     ELAEKYPEQDVNDPQVWEPAFIEVMKQLERPVSMKLTTINNINSKS"
     gene            complement(3320..3691)
                     /locus_tag="ACHOQV_004886"
     CDS             complement(3320..3691)
                     /locus_tag="ACHOQV_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFP9633123.1"
                     /translation="MGETNEILEFDTNNAMNRVAMFAGIPVFALLFLVVIGCILTFSS
                     VWMWGWWGFIVTIPFILAIVGLRIICERDDKAFRRFRFQLRRWKMNRKYGRHLLLTSR
                     NPRWRDKYARRVIQKRILTGK"
     gene            complement(3691..>3981)
                     /locus_tag="ACHOQV_004887"
     CDS             complement(3691..>3981)
                     /locus_tag="ACHOQV_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633124.1"
                     /translation="YIALLFVFIPVYAHAAWYDPIVKFVKEFQTGIIIVGGAVAVGSL
                     VYVGVCWIISRMSGNMETTAMDYVKHIGVIGAVGGAVAAATWAYQLWGGTIS"
BASE COUNT         1200 a          974 c          652 g         1155 t
ORIGIN      
        1 ccaccctcct ataaagtctt ttgctttatc attacatcca gacaaaagca acattaaaaa
       61 gcacaatttc aaaataattt tcatatattc ctcccgttat ataatatgat tatgagcgac
      121 cccacataaa attataaaaa tcctcctcat tctttttatt ttcaattcgt tctttttgct
      181 ccataagata gcgcgtattt tccagttgca ttctctgatt ttcaagctct aacatttcct
      241 gttgatatct taatgctaac tgctccctat cctttggagt ctgaactgaa tttagtttat
      301 ttctcaactc ttcagcatgg tttactcgtg tgactgttgc atcatattgt ttttcatata
      361 catcaacttg agcaagtaac ttatcaaaca atttttgaac atcaggttca tacccggtaa
      421 taccataacg ctttcttaaa tcagttaaat cttgggtttg attatatata tcctgccaat
      481 ctttcattgg cagtaattta tttatatatg gatcattcag aaatgccccc aatttatcgt
      541 ttccctgaag aatcgattta taatgatcgt actgatcttt tgcctgtcga attgcctcct
      601 ttgctttatt aagctgcgcc aacgcttcct gcgcctgttg ctgtgcattt acaactaatt
      661 gcgttaactc tgcaatgctt accgttggca ctccacctgc ataacttaga gaacataacg
      721 ttatagagag acacgcggct atacatgatt tacgtatcat atagaactcc tgtgtttatc
      781 aggaatgaac gttttcgtta acatcaaagc attcctgata tcataaaatc aagactttga
      841 gtttatatta ttgatggttg tcaacttcat agaaactgga cgttccagtt gcttcatcac
      901 ttcaataaat gcaggctccc atacttgagg atcattgaca tcttgttcag gatacttttc
      961 cgcaagctct ttccttactc caatagccac catctcatcc agaaatgggg ccacccatac
     1021 agccggatca tctgtaccta acgcttccct aactcgttta gctacaggta tattggcata
     1081 gtcagatgac aataaaggca accaatattt cagcttatcc tgcatatcca ccttcagaac
     1141 gacagatgca cctccctgtt ttaccagaac cttacgtgaa taagccgaaa actcctctat
     1201 tttactgaac tctttagaag ttacagaaaa tttactgtaa ctgtctgagg tagctttttc
     1261 atttgccatc cattcctgcg taattgtctg ctgaataact gaaggaccac gctcgttatt
     1321 cagaacatat tcaggaacct gagtatccat cataaccatc tcaccacggg tccgccctgc
     1381 atgtaaaatt tccataatgg aattagctgt actctcaaac attagaggtg cccaaaattc
     1441 cgctatactg tttattagta agctgcctgg ttcattctca tgcattcttc tcttcaggaa
     1501 aaagaaagta tttaataata cttccattgc ctgaggatga cgtgtagcaa actccatgtt
     1561 aagaattttt gtacaatcaa atccaagacg gcgaaacaac gtagggtcga aatcatttct
     1621 gggactgtcc aagatccagt aatactgtcc acgtcttcca ttcccatcat cacgacacca
     1681 ctttgataat cgttccctga gtcctccagg accggtatca ggtatagact gtaataacag
     1741 tgaaaaacta cgactttcta catttttatg cttcagaaca gcatcaatcg acatctgtat
     1801 tttagattct tcagtgctgt cagcacctcc ggcacactga acaagaacat caaacagata
     1861 ttgacgtagt tcaaccgaat cttccatttg aaacggttgt atccaggtaa attcaccagg
     1921 ttttatatta aaataacggg ttccaagagc acggagtacg ttctccagcg aattattgta
     1981 atccagagaa aatatcatag gattccagcg actaaaaaac actaattcaa ggctctcaac
     2041 cgttgtttta cctgccccag tcatagcaat tgtcgtttta tgccctggat acttctcacc
     2101 tctacagttc ttccctaaag gactggcgtg tgcattcaga aaatacattc ccccgttttc
     2161 cgtcatcact ggcatcaacg ctgttccgtc tccaggagga ttcccatcgg ctttcccaca
     2221 cggagtcgcg tgtagggaaa atccacaggc caagttttct gtagattttg gcatcgaata
     2281 aatcacatcc gcataaccag gaaatagcgt cagccaggtt acataatttg tacttcggga
     2341 acgaacaaag ccggtatcgc gcgccattaa caacgattgc attcttgctc cgttatcaac
     2401 agcttcttca ggtgtatctc caaatacaat caggctcgca taatattcac caaaaacttt
     2461 tttcccctgg gtaacttcct gaacactatc agcaagttct tcagtttcac tggattgtcc
     2521 ttccgtagac gataaatccg acatctgagt tttcaggcga cgtttcattc ggttacgatc
     2581 ttcaaaaaga aaagattgaa ccatacaaaa gtcatactgt tcatccagca cttcatccca
     2641 cattcctggg taggatttgc tttctggata atcccttaga tcataagtag aagcaaaccg
     2701 gcgctttcca tacgacgggc ggttttccac ataatcgtaa gcgccaaatc ctgtttcact
     2761 gtcaataatc gagtctccca ggcgggtgtc agtaacagga atatctttct cttcaccgtt
     2821 cagaataaac gagtaaaagc ggccaatctg cgaatacagt tttccgtccc gctcctccag
     2881 ctccataaca gaaacaccaa aatcccccag catctctcta cataaagata acaattcccg
     2941 cattcggtta ataccatcgt ccagatcccg aaacttaagg ataagggcca tagaatatcc
     3001 aacttgccgg aatgtcccat tacgaaatgg ttttacccag caatcaacca aatcctgaag
     3061 aacaggtaat tcaagattat actgatgtgt gagatgtata cgacgcttaa aagtgtacgt
     3121 ctgcaatttc aggttattcc cttcctgttt tcccaaagct ttaaaacatc tggcaagata
     3181 cataaactct ttatttagca tagcctgatc tcgcgtatca tgaggaacac cagaaaaacg
     3241 gatcaaactg agaatatgag acgtgcccag agtacaaatt tcaggtgcaa catggtgccc
     3301 caaccagggc accttagcat tactttccgg tgagaatgcg tttttgaata acgcgtcttg
     3361 catatttatc tctccatcgg ggatttctgg aagtcaacag taagtgacga ccatatttac
     3421 gattcatttt ccagcggcgt aattgaaaac gaaaacgacg aaacgctttg tcatcacgtt
     3481 cacaaataat acgcaatcct acgattgcca gaataaaagg gatggtgaca ataaatcccc
     3541 accatcccca catccataca gaagaaaatg tcaaaataca gccaataaca accaaaaaaa
     3601 gcaatgcaaa aacagggatc cctgcaaaca tagccaccct gttcatcgcg ttattagtgt
     3661 caaactcgag aatttcgttt gtctctccca tcagctaatt gttcctcccc acaactggta
     3721 cgcccaggta gctgcggcaa cagcgccacc aacagcacca ataacaccaa tatgtttcac
     3781 ataatccatt gccgttgttt ccatattccc ggacatacga gaaataatcc aacagactcc
     3841 cacgtagaca agagaaccaa cagcaacagc tcccccgaca ataataattc ctgtctgaaa
     3901 ctccttaaca aatttaacta ttggatcata ccatgctgca tgagcatata caggaataaa
     3961 aacaaataat aacgcaatat a
//
LOCUS       DAZKGF010000282         3963 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZKGF010000282 DAZKGF010000000
VERSION     DAZKGF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.282"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(72..818)
                     /gene="cutC"
                     /locus_tag="ACHOQV_004888"
     CDS             complement(72..818)
                     /gene="cutC"
                     /locus_tag="ACHOQV_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP9633125.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGVARVLTSGQKSDALQGLSK
                     IMELIAHGDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(832..1398)
                     /gene="yecM"
                     /locus_tag="ACHOQV_004889"
     CDS             complement(832..1398)
                     /gene="yecM"
                     /locus_tag="ACHOQV_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9633126.1"
                     /translation="MANWHSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1614..3347
                     /gene="argS"
                     /locus_tag="ACHOQV_004890"
     CDS             1614..3347
                     /gene="argS"
                     /locus_tag="ACHOQV_004890"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP9633127.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3400..3792)
                     /gene="flhe"
                     /locus_tag="ACHOQV_004891"
     CDS             complement(3400..3792)
                     /gene="flhe"
                     /locus_tag="ACHOQV_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFP9633128.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPTIEPL
                     RFIWEVSGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(3792..>3963)
                     /locus_tag="ACHOQV_004892"
     CDS             complement(3792..>3963)
                     /locus_tag="ACHOQV_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633129.1"
                     /translation="SRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDN
                     RHIRMTATIGGK"
BASE COUNT          992 a         1068 c         1011 g          892 t
ORIGIN      
        1 gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc gacatgatgc
       61 aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc
      121 gccccgtcta cgacataacg cgaatactcg tccgcgtgtg catctgatga catggacaat
      181 ccttgattac gataacgcat tggtgaggct tgccacgctc ccgcggagct atggacttcc
      241 agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc tccggccata
      301 atgattggag catcaccatg ggcaataagt tccataattt ttgataaacc ttgcagtgcg
      361 tctgattttt gccctgatgt cagtacccga gcaacgccca attccgcaag attattgagt
      421 gtatttaaag ggttagcgca catatcgaag gcgcgatgaa aagtcacggc cagcggaccg
      481 gcagcagcca ttattttttc cattcgtggc atatcgacat tcccgtcaac atcgagaaca
      541 cccgtcacca gtccaggaaa acctaattcg cggactgtgc gcacatcctc aagaatggcg
      601 gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcgaataat aggatgcaca
      661 gggatcgtca cccgctggcg cacggatttc agcaccccca acgacggcgt taagcccccc
      721 tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc cgttagcgcg
      781 cattccatgc tgtaacagca aatttccagt aatgccattt ttgctcctta attacgccga
      841 ctgctcgctg gcaacgatct cttcaatgga ccacggatga aatttaatgg tcgttttgcc
      901 atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc tcaccttttg gcgaactggt
      961 tttaactgag atccccggca ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc
     1021 attcagcgtt tctggatcgc ccggcagaac gatttcaata tgttcccaac cttcgtgcgg
     1081 gtaacgtttt tccccgggcc acggtagctc cacaatggaa aactgccagt gcgcaacctg
     1141 taccggttca tgcaatttaa acagacaaat cggcctgcca ttgatcatat tttctgacaa
     1201 aagctcgccg cactgttcaa acccgcgacg ccagcgctca gcggtggcgt tttgatggca
     1261 acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc aaccccaaac ggcgggaaag
     1321 ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca gctcgtcgat
     1381 agaatgccag ttcgccataa tcactcttcg tcttccacta aaagcgttaa tttaccctgt
     1441 tgccctgcgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt gaaaacgaca
     1501 atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc cgccctaatt
     1561 tctttaactg gtgcgggtaa tttttgctcg cttcatcaat gtaaggtatt ccggtgaata
     1621 ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg
     1681 attgcgaacc gcaggttcgt cagtcagcaa aagttcagtt cggcgactat caagctaacg
     1741 gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca gagcaggtgc
     1801 tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgcgggt cctggcttta
     1861 tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg
     1921 atcgtctcgg tgttgctacg ccagaaaaac agaccattgt ggttgactac tctgcgccaa
     1981 acgtggcgaa agagatgcac gttggtcacc tgcgctctac cattattggt gacgcggcag
     2041 tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg
     2101 gcactcagtt cggtatgttg attgcgtggc tggaaaagca gcagcaggaa aacgccggtg
     2161 aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat tacgacgaag
     2221 atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa actgcaaagc ggtgacgaat
     2281 atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac cagatcacct
     2341 acgatcgtct caacgtgacg ctgacccgtg atgacgtgat gggtgaaagc ctctacaacc
     2401 caatgctgcc gggtatcgtg gcggacctga aagccaaagg tctggcagta gaaagtgaag
     2461 gcgcgaccgt cgtattcctt gatgagttca aaaacaagga aggcgaaccg atgggcgtga
     2521 tcattcagaa gaaagatggt ggctatctct acaccaccac tgatatcgcc tgtgcgaaat
     2581 atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc cgtcagcatc
     2641 aacacctgat gcaggcgtgg gctatcgtcc gtaaagcagg ctatgtaccg gaatccgtac
     2701 cgctggaaca ccacatgttc ggcatgatgc tgggcaaaga cggcaaaccg ttcaaaaccc
     2761 gcgcgggtgg tacagtgaaa ctggccgatc tgctggatga agccctggaa cgtgcacgcc
     2821 gtctggtggc agaaaagaac ccggatatgc cagccgacga gctggaaaaa ctggctaacg
     2881 cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc acggactaca
     2941 tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac atgcagtatg
     3001 catacacgcg tgtattgtcc gtgttccgta aagcagaaat tgacgaagag caactggctg
     3061 cagctccggt tatcatccgt gaagatcgtg aagcgcaact ggcagctcgc ctgctgcagt
     3121 ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtaatg tgtgcttacc
     3181 tgtacgatct ggccggtctg ttctctggtt tctacgagca ctgcccgatc ctcagcgcag
     3241 aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacggcg aagacgctga
     3301 aactgggtct ggatacgctg ggtattgaga cagtagagcg tatgtaatcg cgttatacgg
     3361 caaaagccga tgtcatggca gacatcggct tttttatcat taacgataat tcacaatcac
     3421 ttcattccgt tgtaccttta gcggcggaat taaccgccca ccgccagaca cttcccagat
     3481 aaatcgcaac ggttctatcg tcggtatacc ggaaaaggcc acggtggttc cactctgccc
     3541 ttctaattcg acacaacgag attgtgaaca caagcgaacc cgcagtcctg caggtgtcgg
     3601 gccgataagc tgataacgcc acgccaccag cgtcatcaat cctgcagcag gttgtcgagt
     3661 agaaagaggc gctgacgaca tcgactcacc acggtgattc agcgtaacgc ccacactact
     3721 cgcctgccac atcccctccc cggcggcttg caccagcagc ggaaacaata atattgctaa
     3781 taaagttctc atcatttacc gccaattgtc gccgtcatgc ggatatgtcg gttatcagat
     3841 agttccagat tcgacagcac cactaactgc ggcaggctgc ggcgcaggaa gcgagacaat
     3901 aatggtcgca gcgcgtggtt caccaacaat accggcggcg cacccaacat ctcctgacgg
     3961 gat
//
LOCUS       DAZKGF010000283         3852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZKGF010000283 DAZKGF010000000
VERSION     DAZKGF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.283"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            459..926
                     /locus_tag="ACHOQV_004893"
     CDS             459..926
                     /locus_tag="ACHOQV_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633130.1"
                     /translation="MRDKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSDDRWEAPQR
                     AARLAAAVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRSGSQWLVVEVF
                     GEKGRQHRSADSNPDMVEKMAARYRHAAGQHWSATLAEIERVKRIYQARIKAS"
     gene            1530..1625
                     /gene="dqlB"
                     /locus_tag="ACHOQV_004894"
     CDS             1530..1625
                     /gene="dqlB"
                     /locus_tag="ACHOQV_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFP9633131.1"
                     /translation="MINLLIIVLRAVVAVANALIAILELIRQFID"
     gene            complement(1992..2291)
                     /locus_tag="ACHOQV_004895"
                     /pseudo
     CDS             complement(1992..2291)
                     /locus_tag="ACHOQV_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2597..3262
                     /locus_tag="ACHOQV_004896"
     CDS             2597..3262
                     /locus_tag="ACHOQV_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFP9633132.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHD
                     LLSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVS
                     DIVVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDL
                     DKLLRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIG
                     ILQ"
     gene            3359..3643
                     /locus_tag="ACHOQV_004897"
     CDS             3359..3643
                     /locus_tag="ACHOQV_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP9633133.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVVGKPKLMNFR
                     LPAYFEDILEAEAQRTGQSKTTVLKAALAAFDNLDENLKNHWLLESAKLG"
     gene            3673..>3852
                     /locus_tag="ACHOQV_004898"
     CDS             3673..>3852
                     /locus_tag="ACHOQV_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633134.1"
                     /translation="MRKRDDYYDGLIAGGTVVIAVATLLTLLITVFVNNKENSEFHEW
                     INSHCEKENVENTSFK"
BASE COUNT         1121 a          830 c          874 g         1027 t
ORIGIN      
        1 agagaattga gttgaacgag tggttacatc caggccagct aactcactga acgtaaaggc
       61 tgtacgaaat gttccgaccg tccgataacc atttggctta cttccgtaca tttccttgcg
      121 tagatgtaca gaattgtacg atttcggaca ccaaaaccgc tgttgtcccg ccattctggc
      181 gatttctgaa agttatttga ctaaaaaata gtcttaacgt ggtttatttt ccgaattcca
      241 agcggacccc taagagaaaa gcaataactt aaaggcgata gctgatcacg atgaatcaat
      301 cagttactta tccgcatcga ttaaaaaatg ctcctaacgt acaataattt ccgtttccca
      361 aaccgacccc tacttgatta ggggtagtct tcgccgcttg ttgctgtcag atattaatgc
      421 taatgtatgc atacattagc attaatgagg gatgatatgt gagagacaaa accagggagg
      481 ccatgcggct ttttttaggc ggcaggtgtt acacggctga aaaacttgaa aaggattatc
      541 tggctgaagt tgctaattac agtgacgacc gctgggaagc accgcaacgt gccgcacgtc
      601 tggcagctgc tgtaaagcgt tacaagacat cagaaatgct gcgttttatt tttgctacca
      661 ttgcatacga tcccgatcca gatttaaccc cgctgaccgt gagacgtttg tgcaaggcat
      721 tgtttggtag aagcggaagc cagtggctgg tcgttgaagt ttttggtgag aaggggcgtc
      781 agcaccgcag cgcggacagt aacccggata tggtggaaaa aatggcagcc agatatcgtc
      841 atgccgccgg tcagcactgg tccgcaacgc tggctgaaat tgaacgggta aagcgcatct
      901 accaggcaag aatcaaggca tcctgagacc ggatgcctgg tatttgtctg ggccattaat
      961 gctaatgtat gcatacatta gcattaatgg ccacatcctg gatgaggttc tacgctggaa
     1021 ctggagtctg aagtctaatg gaacgaaaac gtacgttagt gaagtaactg tctgatatat
     1081 caaaacataa tggagaccct caaaccccaa ctacagccga acgacaggca caaaaaaacc
     1141 cgactggtaa tcgggctttt ttgcattttc cagtcttgag ttggtggctc tgaccggaga
     1201 aggttactct acacctaacg tttacatttt attcactatg gtgctgtaaa gcaactaaat
     1261 gtggttaagg aaccacattt agttgtgtaa cgggtgtttt gtgtagcgta taatcagtca
     1321 gttagtcatg tagttggctt gcaatcctgc tcttcccgta tctgcgggta tgaacgattt
     1381 taaagtcctg gctggataaa caggtggtgc tgtattcggt gctctggcag gaggaggtca
     1441 ctctacacct aacaatgcga gagagccgtt gtgacaggca atgtatgagt aagcaacggt
     1501 gatttcctcc agttttactg gagaaacgga tgattaatct gttaatcatc gttctcaggg
     1561 ctgtggtcgc cgttgcaaac gcgctgattg caattctgga actgattcgc cagttcatcg
     1621 actgatgaca gcggaaacgt aactaagggc aggaagtgca ccgcttcctg ccctttaaca
     1681 aaggaggatt tatggatttt atgagcattt tagggcgctg tttacttctt cctcttccgg
     1741 ttgccatatt cattgggtgc ttactttctc aggacgatga ccgatttttt ggcatgttta
     1801 tgagtgtagt aagtgctggg ttgtcgtggt atatcactaa gccaaaacca caaaaggctg
     1861 aggtcgcgta agcggccttt tatttcggta tttattagtt acaacattca cttttctttt
     1921 ttaaagcaac accttagcat agccccaccc atcataaata atgtcagcca gtttattgag
     1981 agcgccctgc cctactcact ggagaattgc cgggatttac gttttattgc gctactgatt
     2041 gtgccacggc tgcaccctag caccttctgt acctgtgtcc aggagctacc gctttctatc
     2101 aacctgataa ttgcatcatg tctggcctga ttaggtttac gctctttata tttgccgtct
     2161 ttccttgctt tctctatccc ctgtttctgg cgttcccgcc gttgttcata atcccttctg
     2221 gcaacagcag ccagcatatc cagcagcata tcgttaatgg ctgaaaacaa tcggttgtca
     2281 aactcactca cactcaaaaa gaaccagaaa acgtacccat aaccagaaag tgacaatgtc
     2341 atacatgtgc tttacagtat aaagataatg taaagataaa tgcagcttta ccacgtaacg
     2401 caaaaataaa gacgcaaata cctttacaaa gtaaaggtct tataaagaca ccattatctt
     2461 tatttgtgct ttactcggta aagcttatgt aatataaaga cacggtaaag acaaaaccat
     2521 ctttacaaaa atcacacatt gtaaagacaa tagagccttt acacattctt tacatcataa
     2581 agagaggaat tctgaaatgg gtaaaatctt gcttgtcgta tcagataagg gtggtgtagg
     2641 caaaagcacc tacgtggcta atacaggctc aatgctggtc aacaaaggta agtcggtaat
     2701 catcctgaag acagataaaa accacgatct gttgagctgg aacgaaaagc ggacagataa
     2761 cggcttactc actatcccgg ttcacgcagc ttatgggaac gtcagcaacg aaattaagcg
     2821 ccttagtaag ctgtgtgaag tgctcattgt cgattgcccc ggccacgaca gccaggagtt
     2881 ccgtagcgcc ctgacagtat cagatatcgt ggtaacacta gttaagccgt cctctgattt
     2941 cgaaagcgaa acactgacca gtgttacaga gaaaatccgc accgctcaga aagctaacgc
     3001 agccctggaa ccgtgggtgc tgttcacaag aatcaactca gctaaacccc gccacagaaa
     3061 agcggccatt gatctggata aattactgcg ctcggacaac atctggattc aacctctgaa
     3121 aacccggata tccgagctgg atgtgtatga aagcgcctgt aatgaagggg caggggtaca
     3181 tgatgttagc cgggcatcaa gcctttcaac agcgaaagca cagattgaac tcgtggctca
     3241 ggaaattggg attttgcagt aatatcaata tgttaaaatt attatcttta cattgtaaag
     3301 ataatgtaaa ggcggtatga tatttactat caatacatac cgtactaggg gaaatttcat
     3361 ggctttaaaa ctaaaccgtc ctaacatcga tgaatctcag caaccagcaa caaacgcaga
     3421 aaccgcgcgt tttatctccg gcgctaccag agcgcctgta gtgggtaaac caaagttgat
     3481 gaatttcaga ctgccagcgt actttgagga tatcctggaa gcggaggcgc agagaacagg
     3541 ccagagcaag acaaccgttc taaaggcagc gctggccgca ttcgataacc tggacgagaa
     3601 cctgaaaaat cattggctac ttgagtctgc aaagttaggt taacaagtaa ttcgcttatt
     3661 taggtttaaa aaatgagaaa gagagatgat tattatgacg ggttaattgc tggaggtact
     3721 gtggtaattg ctgttgcaac ccttctgaca ctgttaatta ctgtatttgt caacaataag
     3781 gaaaattccg agtttcatga atggataaac tcacactgtg aaaaagaaaa tgttgaaaac
     3841 acttcattta ag
//
LOCUS       DAZKGF010000284         3780 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZKGF010000284 DAZKGF010000000
VERSION     DAZKGF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.284"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            21..1406
                     /gene="dcuC"
                     /locus_tag="ACHOQV_004899"
     CDS             21..1406
                     /gene="dcuC"
                     /locus_tag="ACHOQV_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP9633135.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1447..2127)
                     /gene="dpiA"
                     /locus_tag="ACHOQV_004900"
                     /gene_synonym="citB"
     CDS             complement(1447..2127)
                     /gene="dpiA"
                     /locus_tag="ACHOQV_004900"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFP9633136.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2096..3754)
                     /gene="dpiB"
                     /locus_tag="ACHOQV_004901"
     CDS             complement(2096..3754)
                     /gene="dpiB"
                     /locus_tag="ACHOQV_004901"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFP9633137.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIHSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          868 a          995 c          916 g         1001 t
ORIGIN      
        1 gcctggagac aggaaaaatt atgctgacat tcattgaact ccttattggg gttgtggtta
       61 ttgtgggtgt agctcgctac atcattaaag ggtattccgc cactggcgtg ttatttgtcg
      121 gtggcctgtt attgctgatt atcagtgcca ttatggggca caaagtgtta ccgtccagcc
      181 aggcttcaac aggctacagc gccacggata tcgttgaata cgttaaaata ttgctaatga
      241 gccgcggcgg cgacctcggc atgatgatta tgatgctgtg tggctttgcc gcttacatga
      301 cccatatcgg cgcgaatgat atggtggtca agttggcgtc aaaaccattg cagtatatta
      361 actcccctta cctgctgatg attgccgcct attttgtcgc ctgtctgatg tctctagccg
      421 tctcttccgc gaccggtctg ggtgttttgc tgatggcaac cctgtttccg gtgatggtaa
      481 acgttggtat cagccgtggt gcagctgctg ctatttgtgc ctccccggcg gcgattattc
      541 tcgcaccgac ttcaggggat gtggtgctgg cggcgcaggc ttccgaaatg tcgctgattg
      601 acttcgcctt caaaacaacg ctgcctatct caattgctgc aattatcggc atggcaatcg
      661 cccacttctt ctggcaacgt tatctggata aaaaagaaca catctctcat gaaatgttag
      721 atgtcagtga aatcaccacc actgcccctg cgttttatgc cattttgccg ttcacgccga
      781 tcatcggagt gctgattttt gacggcaaat ggggtccgca attacacatc atcactattc
      841 tggtgatttg tatgctgatt gcctccattc tggagttcat ccgcagcttt aatacccaga
      901 aagttttctc tggtctggaa gtggcttatc gcgggatggc agatgcattt gcaaacgtgg
      961 tgatgctgct ggttgccgct ggggtattcg ctcaggggct tagcaccatc ggctttattc
     1021 aaagtctgat ttctatcgct acctcgtttg gttcggcgag catcatcctg atgctggtat
     1081 tggtgatcct gacaatgctg gcggcagtca cgaccggttc aggcaatgcg ccgttttatg
     1141 cgtttgttga gatgatcccg aaactggcgc actcttccgg cattaacccg gcgtatttga
     1201 ctatcccaat gctgcaggcg tcaaacctcg gccgtaccct gtcacccgtt tctggcgtag
     1261 tcgttgcggt tgccgggatg gcgaaaatct caccatttga agtcgtaaaa cgcacctcgg
     1321 tgccggtgct tgttgggctg gtgattgtta tcgttgctac agagctgatg gtgccaggaa
     1381 cggcagccgc ggtcacaggc aagtaaatag tcatgccgga ggcaagtttc ctccggcttt
     1441 atcatgtcac ccactgtggt atatgcgttg tggtctgcca actttgccgt gaacaatttc
     1501 agcaataatc agatggcggc tggcgcaata ttcaagataa cgtctggcag tggtgcggct
     1561 gatagtcagt gcctgcgcca ccgtttctgc cgtatgttgc acaccaggct ctttaaacag
     1621 ttttcgcacc gcgtttagcg tcaaggggtc aatgccggtc ggcagttcgt ctttgggttc
     1681 accgcgagca taggcattaa acatctcatc gatttgcttc tggctggcgc tatcaatact
     1741 ttccagcata tgtttacgct ggcggaaacg ggttagcgtt tgccccagcc gttcataggc
     1801 aattggctta atgagataat caaatacacc acaacgtacg gcttcagaca ccgtttccat
     1861 atcactggct gcagtggtaa acaccacgtc gccgggataa tgcgcctgca ccagttcatg
     1921 cagtaaatta atccctctac cgtcaggaag atagttatcg agcaagatta gccccggctt
     1981 aaaacgctcg atcatcattc gggcctgcgc caggtttccc gccagtaata tctgactgaa
     2041 tccgggaatg tgacgaatat attccgcatg catctctgcc agcggcgttt cgtcctcaac
     2101 gatcaatagg gttaatggag ctgtcattag gtttcacttt cggaatatag attgaaaata
     2161 aggtaccgca gggatcatta tcttcgagag tgataacacc accgcagcgc gttacgtagc
     2221 tggcaatcaa gtacaaccca atgccatgtt cgccgggctc gtcagcacgc gtactgacac
     2281 cctgctcaaa tattttgtct cgtagagact ctggaacgcc gcagccctga tcggcgactt
     2341 caatcaccac atcatcgcct tcatcgctga ggaataattc aacgatcttg tttccttcat
     2401 cgctacgcag gctggcttcg aaggcgttat caagtaaatt gccaacaatg gctgcaaact
     2461 cggtgctgtc cagtcctggc ggcagttgcg aaagctgact tccggggacg atgaccattt
     2521 ttagccccag ttcgcgggcg cgctgcactt taccaaaaag cagccccgcc acctggcgat
     2581 cggcaaacgc ctcgcgcagg ctatcaataa gctgttgctg ggcctgagac tccccctgca
     2641 ccatcgccaa cacgcgatca tactctttca tctgcaacag accattgagc gtcgacatcc
     2701 aattgagatg ctcgtgtcgc aatgtgcgca ggctctcgac gtattgttta atttgcgtca
     2761 gttgtgcatt gagggtggat atttcgtctt tactacgaaa gctgataatg gcccccagca
     2821 aatcatcacc agaatgaata gcttcccggt tggcaataac gctcagaccg ttaaagttcg
     2881 ccaccacatc ctgacgtttt tcatcaatct gttcggtaaa gaaatcggcg ggcctgacca
     2941 cttcagcaat gggtttaccc aaccattgcc gtccggggga gctcagcccc agcatctttc
     3001 ttgcgttacg attgatggcg gtaatgtaac cgtgcggatc caccgcaatg agcccttcat
     3061 aaaccgaact aaacagcgcc tcttgctgac ggaccacgcg tgcgatttgc tttggctcca
     3121 tgcccatcat ctgccgacgg atatgcgcgg ccaggaacca cgacagcaac atcagaatcc
     3181 ctaacagcac gacaaacacg ccagccatcg gtaataaaaa ctcagcccgc cagctatcga
     3241 ttttactcac caggtagcca atcgacacca cgccgatgac ttttccatcg tcatcaaaga
     3301 ttggcgtttt ggcacgcatc gccatgccaa ttgacccttt cccggtgatg aagtagctct
     3361 cccctttctc cagcgcgccc ggtttggtga actgcatagg ataaccaatt ttctccggat
     3421 taggatggta aagacgaatc gagtgccgat cgccaatcac cacataatcg aaatcagtat
     3481 ctctttgtaa tttatcagcg atggtcgcca gcctcttgta gtcacgcgtt ttcactgcgc
     3541 tgatgatact gtcattagaa gcaataattt ttgcctgatt cattgccata tcgcgcacat
     3601 gaagcgttaa atagtcctca aaactggccg taaaatattg cgccagggct gcaatgacaa
     3661 atattgagaa caccagaatc agcaaaaaga tacgcagcgg aaatgccagt ctttggaaaa
     3721 atgcaaactg tttattctcg ttaagctgca acattgtttt ccttgcgggt tgtcccgagc
//
LOCUS       DAZKGF010000285         3725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZKGF010000285 DAZKGF010000000
VERSION     DAZKGF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.285"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..351
                     /locus_tag="ACHOQV_004902"
     CDS             <1..351
                     /locus_tag="ACHOQV_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP9633138.1"
                     /translation="AYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYD
                     LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHP
                     KLQGIAQQNSFKHT"
     gene            507..2042
                     /gene="gadC"
                     /locus_tag="ACHOQV_004903"
     CDS             507..2042
                     /gene="gadC"
                     /locus_tag="ACHOQV_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFP9633139.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2173..3492
                     /locus_tag="ACHOQV_004904"
     CDS             2173..3492
                     /locus_tag="ACHOQV_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFP9633140.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPTGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMVDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
BASE COUNT          920 a          927 c          906 g          972 t
ORIGIN      
        1 gcttatctgg cggatgaaat cgccaaactg gggccgtatg agttcatctg tacgggtcgc
       61 ccggacgaag gcatcccggc ggtttgcttc aaactgaaag atggtgaaga tccgggatac
      121 accctgtatg acctctctga acgtttgcgt ctgcgcggct ggcaggttcc ggctttcact
      181 ctaggcggtg aagccaccga catcgtggtg atgcgcatta tgtgccgtcg cggcttcgaa
      241 atggactttg ctgaactgtt gctggaagac tacaaagcct ccctgaaata tctcagcgac
      301 cacccgaaac tgcagggtat tgcccagcag aacagcttta aacacacctg ataacgttta
      361 acggtaacgg tgtcccgaaa cgaagccgtt tcgggacaat ttccaaagtc tgttcactgg
      421 cattagcaac ggaaaatatt gttctgaata cgcttcagaa caaaacaggt gcggttccga
      481 caggaatacc gttttagggg gataatatgg ctacatcagt acagacaggt aaagctaagc
      541 agctcacatt actcggattt tttgccataa cggcatcgat ggtaatggct gtttatgaat
      601 accctacctt cgcaacatcg ggcttttcat tagtcttctt cctgctatta ggcgggattt
      661 tatggtttat tcccgtggga ctttgtgctg cggaaatggc taccgtcgac ggctgggaag
      721 aaggtggcgt cttcgcctgg gtatcaaata ctctggggcc gagatgggga tttgcagcga
      781 tctcatttgg ctatctgcaa atcgccattg gttttattcc gatgctctat ttcgtgttag
      841 gggcactctc ctacatcctg aaatggccag cgctgaatga agaccccatt accaaaacta
      901 ttgcagcact catcattctt tgggcgctgg cattaacgca gtttggtggc acgaaataca
      961 cggcgcgaat tgctaaagtt ggcttcttcg ccggtatcct gttacctgca tttattttga
     1021 tcgcattagc ggctatttat ctgcactccg gtgcccccgt tgctatcgaa atggattcga
     1081 agaccttctt ccctgacttc tctaaagtgg gcaccctggt tgtatttgtt gccttcattt
     1141 tgagttatat gggcgttgaa gcttccgcaa cccacgtcaa tgaaatgagc aatcccgggc
     1201 gcgactatcc actggctatg ttactgctga tggtggcggc aatctgctta agctctgttg
     1261 gcggtttgtc tattgcgatg gtcattccgg gtaatgaaat caacctctcc gcaggggtaa
     1321 tgcaaacctt taccgttctg atgtcacatg tggcaccgga aattgagtgg acggttcgcg
     1381 tgatctccgc actgctgttg ctgggtgttc tggcggaaat cgcttcctgg attgttggtc
     1441 cttctcgcgg gatgtatgtc acagcgcaga aaaacctgct gccagcggca ttcgctaaaa
     1501 tgaacaaaaa tggcgtaccg gtaacgctgg tcatttcgca gttggtgatt acttctatcg
     1561 cgttgatcat cctcaccaat accggtggcg gtaacaacat gtccttcctg atcgcactgg
     1621 cgctgacggt ggtgatttat ctgtgtgctt atttcatgct gtttattggc tacattgtgt
     1681 tggttcttaa acatcctgac ttaaaacgca catttaatat ccctggtggt aaaggggtga
     1741 aactggtcgt ggctattgtc ggtctgctga cttcaattat ggcgtttatt gtttccttcc
     1801 tgccgccgga taacatccag ggtgactcta ccgatatgta tgttgaatta ctggtcgtga
     1861 gtttcctggt ggtacttgcc ctgcccttta ttctctatgc tgttcatgat cgtaaaagca
     1921 aagcgaatac cggcgtcact ctggagccaa tcaacagtca gaacgcacca aaaggtcact
     1981 tcttcctgca cccgcgtgca cgttcaccac actatattgt gatgaatgac aagaaacact
     2041 aaccatgtct gaatggttat aagacaaggg agcgataatt catcgctccc tttttcgtgc
     2101 ttgttgtcgt attgacttaa ccgggataag tacgagaatg agcgcacatc tgtttaccgg
     2161 aaaccagcac atatggatat ctgctcccga aacgagaaat tagcgattag aagaccagcg
     2221 atactggttg cactggcact tttactgtgt agttgcaaaa gcacgcctcc agagtcaatg
     2281 gtgacaccac caacaggttc gaagccacca gctacgacgc aacaatcgtc acaaccgatg
     2341 cgtggcatct ggctggccac agtgtctcgc ctcgactggc caccggtttc ctcggttaac
     2401 attagtaacc ccaccagccg ggcccgtgta caacaacagg cgatggtcga caaactggat
     2461 catctgcaac gtctcggcat caacacggtc tttttccagg tcaagccgga cggtaccgcc
     2521 ctgtggccat caaaaatttt gccgtggtcc gatcttatga ccggaaagat tggtgaaaat
     2581 ccaggttacg atccgctgca atttatgctc gacgaagccc acaagcgtgg gatgaaagta
     2641 cacgcctggt ttaaccccta tcgcgtatcg gttaatacga agcccggtac tatcagggaa
     2701 ctgaatagca ctctgtctca acaaccagcg agcgtctatg tgcaacaccg cgactggatc
     2761 agaacgtccg gcgatcgctt tgtcctcgac ccgggcatcc ctgaagttca ggactggatc
     2821 acatcaatag tcgcggaagt ggtttcccgc tatccggtag atggcgtgca gtttgacgac
     2881 tatttctata ctgagtcacc gggttcacgg ctaaatgata acgaaacgta ccgcaaatac
     2941 ggaggcgcat ttgcgtcaaa agcagactgg cggcgcaaca atactcagca gttaattgca
     3001 aaggtatcgc acactattaa aagcattaag ccggaagtcg aatttggcgt tagcccggca
     3061 ggcgtgtggc gtaaccgatc acacgatccg ctcggttccg atacccgagg cgcggcagcc
     3121 tatgacgaat cctacgctga cacccgtcga tgggtggaac aaggattgct ggattacatt
     3181 gctccccaaa tttactggcc gttctcacgg agtgccgcac gttatgacgt tttggcaaaa
     3241 tggtgggcgg atgtcgttaa accgaccagg acccgcctgt atatcggtat cgccttctat
     3301 aaagtgggtg aaccttcaaa gatagagcca gactggatga ttaacggcgg cgtaccggaa
     3361 ctgaaaaagc agctcgatct taacgatgcc gtgccagaaa ttagcggcac aatcttgttc
     3421 cgtgaggact atctgaataa gccacagact caacaagcgg tcagctatct gcaaagtcgt
     3481 tggggcagtt aaactcccat tcttactttg taacaagccg gtgctttccc cccggcttgt
     3541 taccctcttg aaatatccca ctagtaatca tgcttactta agtcaaatta actacgcaat
     3601 taaccacact tagttaaaca gtcataaatc ccctaataaa aatgtatgaa tatgaaaaaa
     3661 catacgatat tcgctgtcac cgaaaaataa ttacgatggg ctatactctt ctcaagaaca
     3721 gtagc
//
LOCUS       DAZKGF010000286         3715 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZKGF010000286 DAZKGF010000000
VERSION     DAZKGF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.286"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            252..803
                     /gene="ybcL"
                     /locus_tag="ACHOQV_004905"
     CDS             252..803
                     /gene="ybcL"
                     /locus_tag="ACHOQV_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFP9633141.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATVTYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            813..1610
                     /locus_tag="ACHOQV_004906"
     CDS             813..1610
                     /locus_tag="ACHOQV_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9633142.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHTLTVDNNQIILFPCGYEFYIENYPEAGLYLAEMLYLPIDLIESFQKLYTVTDQIR
                     NKTSFFLPQNPELIYCWEQLKTSVSRGFSTKIQEHLAMGVLLSLGVNHVNHLLLSYSK
                     QSLISRCYNLLLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFNITPREIRKASRE"
     gene            1727..1828
                     /locus_tag="ACHOQV_004907"
     CDS             1727..1828
                     /locus_tag="ACHOQV_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633143.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            1825..2280
                     /gene="ybcN"
                     /locus_tag="ACHOQV_004908"
     CDS             1825..2280
                     /gene="ybcN"
                     /locus_tag="ACHOQV_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFP9633144.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            2280..2450
                     /locus_tag="ACHOQV_004909"
     CDS             2280..2450
                     /locus_tag="ACHOQV_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9633145.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            2443..2733
                     /locus_tag="ACHOQV_004910"
     CDS             2443..2733
                     /locus_tag="ACHOQV_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP9633146.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            2730..3092
                     /gene="rusA"
                     /locus_tag="ACHOQV_004911"
     CDS             2730..3092
                     /gene="rusA"
                     /locus_tag="ACHOQV_004911"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP9633147.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            3089..3229
                     /locus_tag="ACHOQV_004912"
     CDS             3089..3229
                     /locus_tag="ACHOQV_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP9633148.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            3315..3698
                     /gene="quuD"
                     /locus_tag="ACHOQV_004913"
     CDS             3315..3698
                     /gene="quuD"
                     /locus_tag="ACHOQV_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFP9633149.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
BASE COUNT         1066 a          742 c          877 g         1030 t
ORIGIN      
        1 ttattatgag caaaatggtg tatgtcacgt attttgaatg gtaggttaaa aaataacacc
       61 gactttcgta ggtattacta ataataaagc agagttttta gatagtatca atgtgctttg
      121 tgtatattgt ggcaaataat tgggttgggg gtacaattgt gattgctttt gcatgaacat
      181 tgcgccttta tgcataatga gataaaggaa tatcaaataa aataacgata ggtcataaca
      241 aagaggtttt tatgaaaaca cttatcgttt caactgtatt ggcattcata acattttctg
      301 cgcaggctgc agcatttcag gtcactagta atgaaataaa aacaggagag caacttacaa
      361 cgtctcatgt cttttctgga tttgggtgtg aaggtggtaa tacatcgccc tcattaacct
      421 ggtctggtgt tcctgaaggt accaaaagct ttgccgtaac tgtatatgat ccagatgcac
      481 ctacaggcag tggttggtgg cattggactg ttgttaatat tccagcaaca gtaacatatt
      541 tgcccgttga tgcagggaga cgtgatggaa caaaactgcc gactggtgct gttcaaggcc
      601 gaaatgattt tggctatgct gggtttggtg gcgcatgtcc tcctaaagga gataaaccac
      661 atcattacca gtttaaagta tgggctctaa aaactgaaaa gattcctgta gattctaact
      721 ccagcggagc gttagttggt tatatgctta atgctaataa aatcgcaacc gctgagataa
      781 caccagttta tgagataaag tagggtgaga gtatgctggc aagaggtaag actaacttaa
      841 agatcgaaga aatacggatg cataaacatc atgagattca tagggttaag cctcttatgc
      901 cagctttgtg tcgtatccgt cagggaaaga aagttatcaa ttgggagacg catactttaa
      961 ctgttgataa taatcaaata atattatttc cttgtggtta tgaattttat attgagaatt
     1021 atcctgaagc agggctttat cttgcagaaa tgctttactt acccattgat ttaattgaga
     1081 gtttccaaaa actttatacg gtaactgatc aaatacgtaa caaaacaagt ttctttttac
     1141 ctcagaatcc tgagttaata tattgttggg agcaactaaa aacatctgtt tcccgaggct
     1201 tctcaactaa aattcaggag cacttagcaa tgggcgttct actttcgtta ggagtgaatc
     1261 atgttaatca tttactttta tcatatagta aacaatcatt gataagtcgt tgttataacc
     1321 tgctgctatc cgaacccggc acaaaatgga cagcaaacaa ggttgctcga tatctctaca
     1381 tttctgtttc tacattacat cgccgtctag caagcgaggg ggtaagtttc caaagtatac
     1441 tggacgatgt gaggttaaat aatgcgttgt ctgctataca aacgacggta aaacctataa
     1501 gcgagattgc cagagaaaat ggttataagt gtccttctcg ttttactgaa agatttcata
     1561 atcgttttaa tataacacca agagagataa gaaaagcttc cagagagtaa aagtgtttta
     1621 agaaggagca attctatcga ttttgatttt gggaaatcaa cacggcataa ttatgtcacc
     1681 ggagcctgaa caactccggt gacttctgcg ctaaacgggg acgtttatgc gcacatacaa
     1741 tccaaactct cttctccctt cacagatgca gaaatgcacc tgcaattctt tgcatctagc
     1801 gtttgacctc tgcggaggtg aagcgtgaac ctctcacaag acggcatcaa attacatcgc
     1861 ggcaacttca ccgctatcgg tcggcagatc cagccttatc tggaggaggg caaatgcttt
     1921 cgcatggtgc ttaaaccgtg gcgtgagaaa cgcagtcttt cccagaatgc actcagccac
     1981 atgtggtaca gcgaaatcag tgaatacctc atcagcaggg gtaaaacgtt cgccactcca
     2041 gcttgggtaa aagatgctct caaacacaca tatctcggtt atgaaaccaa agacctggtt
     2101 gatgtcgtaa ccggtgatat caccactatc cagtcgttac gccatacctc cgatcttgat
     2161 accggagaga tgtatgtctt cctgtgtaag gttgaagcct gggcggtgaa tattggctgc
     2221 cacctgacta ttccgcagag ctgcgagttc cagctgctcc gcgacaagca ggaggcgtaa
     2281 tggctacacc gcttattcgt gtcatgaacg gacacatcta cagagtatca aatcgtcgta
     2341 agcgtaagcc tgagctgaag ccatccgaaa taccaacact gctcggatat accgctagcc
     2401 tggttgataa aaaatggttg cgactggcag caaggaggaa tcatggctga tttgagaaaa
     2461 gcagcgcgtg gtcgggaatg ccaggtaaga atccctggcg tatgtaatgg caattctgaa
     2521 acgtctgtac tggcacatat ccggctggct ggattgtgcg gtaccggtat caaaccgcca
     2581 gacctgattg ccaccattgc atgttctgcc tgccacgacg aaatcgaccg ccgcacacat
     2641 tttgtcgatg ctgcatatgc aaaagaatgc gcgctggaag gtatggcgag aacacaggtt
     2701 atctggctga aagagggggt tattaaggcg tgaataccta cagtatcaca ttaccctggc
     2761 ctccgagcaa taatcgctat taccgccata atcgcgggcg cacgcacgtc agtgcagagg
     2821 ggcaggcata ccgcgataac gtcgcccgaa tcattaaaaa cgcaatgctg gatatcggcc
     2881 tggctatgcc tgtgaaaatc cgcattgagt gccacatgcc ggatcgccgt cgccgtgacc
     2941 tggataatct gcaaaaagcc gcttttgacg cactcaccaa agcaggtttc tggctggatg
     3001 atgctcaggt cgttgattac cgcgttgtga agatgcctgt taccaaaggt ggcaggctgg
     3061 aactgaccat caccgaaatg gggaatgaat gatgtttgag ttttatatgg cagaacttct
     3121 tcgccaccgc tgggggcatc tgcgcttata tcgtttcccc ggttctgttt tgaccgatta
     3181 ccgaatactg aagaattacg ccaaaaccct gacaggagca ggagtatgaa gtcagagata
     3241 acaatcaact aatactgttt tgttgatttt tgcttgtaat tggcgttctg gtctgatttt
     3301 tgtggagtaa gttgatgcgt gatattcaga tggttcttga gcgttgggga gcgtgggcgg
     3361 ctaataatca tgaagatgtg acctggtcgt ccattgccgc cggttttaag ggattaatta
     3421 cttcaaaagt aaaatctcgc ccgcaatgtt gtgacgatga cgcgatgatt atttgcgggt
     3481 gcatggcccg tctgaaaaag aacaacagcg atttgcacga tttattagta gattattatg
     3541 tagtcggtat gacattcatg tcactggcag gtaagcattg ctgctctgat ggttatatcg
     3601 ggaaaaggtt acagaaggct gagggcataa ttgaagggat gttaatggca ttagatatcc
     3661 ggttagagat ggatatcgtt gttaataact ctaattaata tgccaattgt ttact
//
LOCUS       DAZKGF010000287         3707 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZKGF010000287 DAZKGF010000000
VERSION     DAZKGF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.287"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            168..351
                     /locus_tag="ACHOQV_004914"
                     /pseudo
     CDS             168..351
                     /locus_tag="ACHOQV_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(393..920)
                     /locus_tag="ACHOQV_004915"
     CDS             complement(393..920)
                     /locus_tag="ACHOQV_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6882 domain-containing protein"
                     /protein_id="HFP9633150.1"
                     /translation="MTNLFKMLFGRKNKDSSANTAILSEPDLTIARANNEMQLLTQAA
                     IEMWGLDTAAWDVDLNTGTITFTNDEKEIMVTAPVQVVGTYDTEDSTWLWGWDHPSVS
                     ESLGEYARRVHDFGEQYGKETLTTRKIAASMDDAWEFTALACYLGDGQGGYRGVSGST
                     CVFMVYGPVTLNKKD"
     gene            complement(1317..2878)
                     /locus_tag="ACHOQV_004916"
                     /pseudo
     CDS             complement(1317..2878)
                     /locus_tag="ACHOQV_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611652.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3220..3444)
                     /locus_tag="ACHOQV_004917"
     CDS             complement(3220..3444)
                     /locus_tag="ACHOQV_004917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633151.1"
                     /translation="MNVENNQLGGSEWLQTGKAREHGAYVLSCSDNPSGEACKRGQAE
                     NKAYAAALATGGCYRGVPKLCGYLVPEQMP"
BASE COUNT          986 a          918 c          717 g         1086 t
ORIGIN      
        1 tcgaatcctc ataaccaaat tcaaccccac agcaattaca tatatcgtat gttggtgtct
       61 caccatcatc tccccatggt gcttctggct gttcagcacc acatactcga caaacataaa
      121 attcattatt tttgtgcatt aaaataatcc agatttattt gataacagtg ttcttcggca
      181 gctattacag cagaaaacgt ggtgcactgc gcacggacta atcggaacct atcggctgag
      241 cgatatcgat ccggaagcgt atctgcctca tatactgaga gttttgccgg aatggccttc
      301 caacctgttg atgaactacc tccatggaac gtagtgctca ccaataaata agcgtcatgc
      361 ctaacttaaa gtaaatagaa tagcagatat gcttaatcct ttttgttgag tgtcactgga
      421 ccatagacca taaaaacaca agttgaacca gatactcccc tataacctcc ctgtccatcg
      481 ccgagataac aggcaagagc agtaaattcc caggcatcat ccatagacgc agcaattttg
      541 cgtgtggtta gtgtttcttt gccatattgc tcaccaaagt cgtgcacgcg ccgggcatat
      601 tctccaagtg actcgctgac tgacggatgg tcccaccccc ataaccaggt gctgtcttca
      661 gtatcgtaag tgccaaccac ttgaacaggt gctgtgacca tgatctcttt ttcatcattg
      721 gtgaaggtga ttgtacctgt attaagatca acatcccacg cagcagtatc aagtccccac
      781 atttctatag cggcttgtgt gagtaactgc atttcgttat tcgctcgtgc aattgtgaga
      841 tctggctctg aaagaattgc tgtgttagca ctggaatctt tattctttct gccaaaaagc
      901 atcttgaata aattagtcat tcctactctc ccgttttctg caaacacttt ggctgaatta
      961 ttaacgttca aattttgtca gacatgtgta taccctaacc tgacatcagg cctgataatg
     1021 aaaaactgtc agaataaaat cgtcatgagt atttattgag acaagtaatt aaaggtcata
     1081 gattcattta tgacatattc aggcgtgata gcggcatcgt ggtagtctac gtatgggcca
     1141 agtaatgtct cagatcagca ttaaacttag ggttccagcc acctttcctc ctgtttgccc
     1201 cttcattacc cccccatgaa agtcccttac caatgccata atcaatacca gaagcaccac
     1261 cattgataag tgctgcgtat tttcatggaa catcgatcag tgattcgcgg cgtttttcaa
     1321 ggtagttgtc gcctgatgta ataaaattcc gcttcttttt ctggatttag ataaactgcc
     1381 tgaatgagtt cccagtcacg taactgtttg ctccagcgct caggatgacg tttgtgtgct
     1441 ccgcgataaa ctgtttttct ggcttccagt atttgtgcat caattccgcg atggcgttca
     1501 tccggagtaa catacttaat accgctgtgc ttgtgttcca ggttgtacca tcgcgtgaac
     1561 ctgcttaacc aacttctggc ttcatctatg gttcgaaaac cattctctgg ccactatgga
     1621 caatacttaa tcgtccggaa gagcgattcg gaataagggt tatcattgct aacccgggga
     1681 cgaaaataag aacttaatac tccaagcgcg tacattttgt ccagtaacgt acagctgcgc
     1741 attggccctc cattatcaga atgaagcacg agattgcggg atgaacattt ttcattccat
     1801 acggcacgtt gaagcagttc tgccgcatac tgcccagatt ctttcgaaaa tacttcggct
     1861 gcaacaattt ttcggctata aatatcgacg atcatataga gataaaaatg ctgcccctta
     1921 acccacgatg ggacataact aatatcccag ctccacactt ggttgggtgc tacagctttg
     1981 agtacttcag gccgcttata ttggtgttca cgggttctcc ttgtcagttg gttagctgat
     2041 tttaatacgc gataaaaagt tgattctgaa gcgatataac cccctctgtc ggcaagagtt
     2101 ggaacaataa tattcggaga caatgatgca aactccggag aattgcatac atcaagtatt
     2161 cttaatcttt ctgtttctga tagtttattt cccggggcag gccttgatgc acctggtctc
     2221 tgatctgcat gaaccacacc accctgtatc caacgttgaa gtgttctgac tgaaatacca
     2281 atacactggc aggcttgtgt ttttctcgct cctgaactga cagcctcgct gaccagatta
     2341 ataatcttct gccgctctgg aagaggtgtc agccttcctc actgttgtcc cagagggcat
     2401 tgaacttttc ccttaacatc aaaagagcag ctgcttccgc cagggcttta tctttacgga
     2461 gaagttcttt ttctagttct cgtaaccggg tctttgcttc acgtaattct ttatcagcac
     2521 ggcgattttc tgtcattttc atttcatgag cctgaatgga tatagctctc cattctttaa
     2581 tttactcaac aaataacccc attctccgac aataatcagc cagttccatt tcagataatg
     2641 ttgcagtttc aattacaaca gaaaatcgtc gctctgatgt ccattcattt ccatcgatag
     2701 aagatatttt ttcattttca aacaggcctt gttgcaccag ttcaagtctc catcttgcta
     2761 ttactgaagg gctcaggtcc agtttacggg caatatgccg tatggaccaa ctataaggtg
     2821 gtaataacca ggtaacacct tgttctttta tatcaattgg taccgcatgt gcaggcatag
     2881 cctctctcct gtgtaaaaat ctaggcgaca actatcctga cacagggggc cattgaccga
     2941 aggagatcag tgaaggtaat ttaaaaacgg ttctgtgaaa ctgacgcgaa ccgatctcca
     3001 tcgatgttac cgatctccat cgatgttacc gatctccttc acggtaatac gcaagtgcac
     3061 cagacagagg atctttgttg tcaatccagt tacccaacgc accacctgcc gcattctagg
     3121 ctattgtccc tttccagccc tgccccatgg ttatcgcatt tatccagcct gctgccactg
     3181 agttgaccga gtttatttca ccactatctg aatactgcat cacggcattt gctccggcac
     3241 caagtaacca catagcttgg gcactccccg gtaacagcca cctgtcgcta atgcggcagc
     3301 ataagcttta ttttcagcct gcccccgctt acaggcttca ccggaaggat tatcactaca
     3361 ggagagaaca taagcaccat gctctctggc ttttcccgtc tggagccatt cactgccacc
     3421 cagctggtta ttctcaacat tcacctacag gtgaacagcc tccaacggtt caacataacg
     3481 caaattatgt atagttcaga acaacgaacc aagcataatt tgcgttatgt ctggaaccca
     3541 acagatgttt gcaactgtac tgtctgcgtt atatcaaaag tcactcagag tcaggcggtg
     3601 ttttccttga tttatatctg gctatcagtt tagaaaaaac aatcccaacc catgcagtta
     3661 acccggtaat aacaccaaaa taaacagccc tttttatttg attgatt
//
LOCUS       DAZKGF010000288         3705 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZKGF010000288 DAZKGF010000000
VERSION     DAZKGF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.288"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..980
                     /locus_tag="ACHOQV_004918"
     CDS             <1..980
                     /locus_tag="ACHOQV_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9633152.1"
                     /translation="HDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRVKQL
                     ARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHIAKK
                     LLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLVARV
                     KVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCVGNS
                     SERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEPEED
                     ADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            complement(1295..1717)
                     /gene="subB"
                     /locus_tag="ACHOQV_004919"
     CDS             complement(1295..1717)
                     /gene="subB"
                     /locus_tag="ACHOQV_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFP9633153.1"
                     /translation="MTIKKFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKALTSNALVGLSTCATSTECFGPDRKKN"
     gene            complement(1734..2777)
                     /gene="subA"
                     /locus_tag="ACHOQV_004920"
     CDS             complement(1734..2777)
                     /gene="subA"
                     /locus_tag="ACHOQV_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFP9633154.1"
                     /translation="MLKNLRLHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKEPEALYIHGTAMASLIASRHE
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWSELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSVNKKQDPVIRV
                     AALAQYRKGETPVLHGGGVTGSRFGNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYSSLADKVTEGRVLNAEKAISMFCKKN
                     YIPVRQGRMNEEL"
     gene            complement(3507..>3705)
                     /locus_tag="ACHOQV_004921"
     CDS             complement(3507..>3705)
                     /locus_tag="ACHOQV_004921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633155.1"
                     /translation="RHAGYIIRESGSERRYLLPQSYLINPYQQGNSMAKSSNYAPVKV
                     VIYVDIGKDPCHTVIINSSDN"
BASE COUNT         1045 a          821 c          826 g         1013 t
ORIGIN      
        1 gtcatgattt taatgaaaaa atagatttct ggtatgtgat agctctcgca ggacagtcta
       61 atggtatggc ctacggtgaa ggtattcccc tgccagatac actcgacaaa cccgagtccc
      121 gtgtaaaaca actggctcgt cgaaaaacaa tcacaccagg aggaaaagag tgtaaattca
      181 atgagattat tccggcagat cactgcctgc atgatgttca ggatatgagt ggatatcacc
      241 atccggctgc tgatttgcat aaaggggaat atggttgtgt cggacaggga ttgcatatag
      301 ctaaaaagtt actgccatac atacctgagc aagcagggat attactggtt ccttgttgca
      361 gaggaggtgc cgcctttact gttggagctg aaggtatgta tgtacctgat acaggggcaa
      421 cagctgatgc aatgcggtgg ggcaccggaa cagcactcta tgaggactta gtagcccggg
      481 ttaaggtagc tctggaatat aacagaaaga ataaactgct atctgtctgc tggatgcagg
      541 gggagtttga tcttatgtca ccagattatg aaaagcatcc tgacttgttt tatcagatgg
      601 taacttcttt caggtctgag ttatctgaat actcatctca gtgtgttggt aattcgtcag
      661 aaagagttcc atggttgtgt ggtgatacaa cctggtactg gaaggaatct tatcaaaaag
      721 aatatgattt tatatatgga cattacagac aacggactga tgatgaaata cattttctca
      781 gctttcagga tagtaaccga catgaattaa ctaatgagcc agaagaagat gccgatgatt
      841 tatctgtcgg gtatctgggg tcatcatgga ggacagaatt aagctggaca acgtcacaga
      901 gaagtacaca ctttaattca atggccagaa ggggagttat tgctgaatgt tatgcgcaaa
      961 aaataagaaa ttatttgtag acaagttcag gatatatcta cagctctgac atgattctct
     1021 gagagacatg acggagtggt atggagttgt cggtatcaac accaggatag ctggtcgtta
     1081 ccgtttgata tcactggtgc tgatactatt tggacggtag tgtgtcacga aggcacgcac
     1141 cagggacgtg agtccctgta atatcatcga cagtcccatt atggcatgtc tgcacctaca
     1201 acgtcatttc gaggtcggct cagtgttcac tcgagtctta tatcactgac gccgacactc
     1261 catccagagt atctctggac ggaaacatca cctattagtt cttttttctg tcggggccaa
     1321 aacattcagt cgatgtggcg caggtcgata atccaacaag ggcattggac gttaatgcct
     1381 tcacaaaggg tggatacgtc cataccccgg gttcataata aatcctgacc aactgacctg
     1441 ttgtgtaaaa atatagtgcc tgattatata atgtggaaaa cgaaggcccc cagacagatg
     1501 aattcttcat cgagcaggct cttatggaag aaccaccagg ttgtttccct tcaatacaaa
     1561 aataagggtt attatctatt tgccctgtat gaaactgatc aataacaacg cctgaaaaca
     1621 tgccatcccg ggcatcgcca gtccactctg ccatggcagg attaagggat aaacatccca
     1681 tgacacctgc acatacaaaa aacttcttaa tagtcatatt cacctccgaa gttttacagt
     1741 tcttcattca tccttccctg acggacagga atataatttt tcttgcagaa catgctaatt
     1801 gctttttctg cattaagaac cctcccttcc gtcaccttat ctgcaagtga ggagtatttg
     1861 tctgcagact ccagcagtgt acgcttcagt tctgtcgctg ttgcacgggg attacatgag
     1921 accatcgctg ccagaacgcc ggaaactata gctgttgctt ccgaggttcc actaccgatc
     1981 cccgtttttc catctggtct gaggaatgta atattctgtc ccggtgcggc aatatcaacc
     2041 cagccgtttc cgaaccgact tccggtgacc cctccaccat gcaatacagg tgtttctcct
     2101 ttccggtact gtgccagagc tgccactctt atcacaggat cctgcttttt attcacactg
     2161 ctgaccggat ggtaagccgc aggccatatt ctctgctgag cactcagttt tcgtatatct
     2221 gcgccatcat tacccactgc cgcaacaatt aatcgctcat tattccggcc catacgggaa
     2281 agcagttcgg accagaccga tgcggaagat atccccttct ggcctcccga tatattaatg
     2341 attttctctt ctcctggtgc aagaaaaaca tttgacataa tgctttcagt tgctctaatc
     2401 catgagtcct gtacaccgtc aggaataacc cttctgctgg atatcagtgc atgaggataa
     2461 acaccataaa cctcatgacg tgaggcaatg agtgaagcca tggcagtacc atgaatatat
     2521 aaagcttcag gctcttttac cgggaatggc gaaccatcct gagtaaaact gaatttggca
     2581 aattcgctgt cgctgaggcc tcctacaaat gccactccgg aatcaacaac agatacgaca
     2641 accggggtat ttttgtctgt gagcgacatg gttgtttctg tcagaccaat tgcatcaaaa
     2701 taccatggtt tttccgccct gactgacact gagataaaaa gcaggaacag aatatgcagc
     2761 cgtaaatttt tcagcacaat atcctccagt ggtacaaata aaaagtattg ctggtcaata
     2821 tcttacagga acatcacatc tgaaatatcc tgttttcgac aaaagataaa caatcacaat
     2881 caataaagcg agtgaagtca ttctcaaaaa cattaattta tttgcatatt caatagctca
     2941 ctgaactcct tcacggtaat atgcactagg atttctgcaa taggtaatcg gtgagatgct
     3001 cttcctctct tcctactcct tcagagcaga agtaaatcca gctatgatgg gtaaaaatta
     3061 gccagaaaaa ataagagggg gcaactaagt tgcctggcat gtttatgaca ataaagagta
     3121 ccgtcatgca tcatggcgaa caggaaatta cagtgccgtc ttaccggggc gatcagcgca
     3181 tggttgtgtc gtttaccctg gctcattatt acgtgtgcag taagccccgg atatcaggtc
     3241 actgagtata tctttgaatg agcggtaaac cggggatgcg atctgctgcc cgggaaagtc
     3301 gaactgactg gtcaatagat atcaatacgt ggcagccagg ccgtttacag gtatctgaca
     3361 aggacacctt ctgagcgtgc aatgtggacg ggtaatacaa cgatggtagc aggctggtca
     3421 gcaatcagca gtatatgccc atatttcctg aggccggata tctgtttcct gagtttgttt
     3481 tcgtcgtttg ctaatatttt gtcgaactaa ttatcagaac tattaatgat aacagtgtgg
     3541 cagggatctt taccgatatc aacgtaaata acgaccttaa caggtgcgta attgctggat
     3601 tttgccatgc tgttaccctg ttgatacggg ttaattaaat aactttgggg aagcaggtac
     3661 cggcgttcac taccactctc cctgataatg tatcctgcat gcctg
//
LOCUS       DAZKGF010000289         3701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZKGF010000289 DAZKGF010000000
VERSION     DAZKGF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.289"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..1325
                     /gene="alaC"
                     /locus_tag="ACHOQV_004922"
     CDS             87..1325
                     /gene="alaC"
                     /locus_tag="ACHOQV_004922"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFP9633156.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYGIEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(1390..1461)
                     /gene="ypdK"
                     /locus_tag="ACHOQV_004923"
     CDS             complement(1390..1461)
                     /gene="ypdK"
                     /locus_tag="ACHOQV_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFP9633157.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(1817..2737)
                     /gene="lpxP"
                     /locus_tag="ACHOQV_004924"
     CDS             complement(1817..2737)
                     /gene="lpxP"
                     /locus_tag="ACHOQV_004924"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFP9633158.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3090..3332
                     /gene="yfdY"
                     /locus_tag="ACHOQV_004925"
     CDS             3090..3332
                     /gene="yfdY"
                     /locus_tag="ACHOQV_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFP9633159.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(3409..3636)
                     /locus_tag="ACHOQV_004926"
     CDS             complement(3409..3636)
                     /locus_tag="ACHOQV_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633160.1"
                     /translation="MACNVFAFSISGGRSETSYKETEKTSVMTTTHSTKLQPSQAILF
                     KLREDAPPLNLTEDITPPFSTKANYLIHPVR"
BASE COUNT          937 a          873 c          943 g          948 t
ORIGIN      
        1 aaattaatca ggctattttc aaaacagcca ccatcgtggc agcgtcgctc ggacggtccg
       61 ggcgctaacg ttaatctgag gaaattatgg ctgacactcg ccctgaacgt cgctttacgc
      121 gcattgatcg tctcccgccc tatgttttta acattaccgc tgaactgaaa atggctgcgc
      181 gtcggcgcgg cgaagatatt attgatttca gtatgggtaa cccggacggt gcgactccgc
      241 cgcatatcgt cgaaaaatta tgtacggtgg cccagcgccc ggacacgcat ggttactcca
      301 cgtcacgcgg cattccgcgg ttacgtcgcg ccatttcccg ctggtatcag gatcgctacg
      361 gcattgagat cgacccggaa tcagaagcca tcgtcactat tggttcgaaa gagggactgg
      421 cgcatctgat gctggcgacg ctggatcatg gtgacactgt actggtgcct aatccaagct
      481 acccgattca tatttacggc gcggtgattg ccggggcgca ggtacgctca gtgccgctgg
      541 tggaaggtgt cgatttcttc aacgaactgg aacgcgccat tcgcgaaagt tatccgaaac
      601 cgaagatgat gatcctcggc ttcccgtcga atccaaccgc gcaatgcgtg gagctggagt
      661 tcttcgaaaa ggttgtggcg ctggcgaaac gctacgatgt gctggtggtc catgacctgg
      721 cctatgccga tatcgtctac gatggctgga aagcgccgtc aatcatgcag gtgccaggtg
      781 cacgcgatgt ggcggtcgag ttctttacgc tgtcgaaaag ctacaacatg gcgggctggc
      841 gtatcggctt tatggttggc aacaaaacgc tggtcagcgc gctggcacgt attaaaagct
      901 atcacgatta cggcaccttt acgccgttgc aggtggcagc gattgcggcg ctggagggcg
      961 atcaacagtg cgtgcgcgac attgctgaac agtacaaacg ccgccgtgat gtactggtaa
     1021 aagggctgca tgaagcgggc tggatggtcg aaatgccgaa ggcttcgatg tatgtctggg
     1081 cgaaaatccc ggaaccgtat gctgccatgg gctcgctgga atttgccaag aagctgctga
     1141 acgaagcgaa ggtctgtgtc tcgccgggga ttggctttgg cgactacggc gacacgcatg
     1201 ttcgctttgc actgattgaa aaccgcgacc gtattcgtca ggcgattcgt ggaattaaag
     1261 cgatgttccg tgccgacggt ttactacccg ccagcagcaa acatattcac gaaaacgcgg
     1321 aataaacacc atcgccagaa atgcaaacag gagccagatg gctcctgttt ttcattgcac
     1381 agtttgcttt tagatcatca gggcaaaagt accggcccaa acgatgacca taaaagaaat
     1441 gcccataaag aaatatttca ctttacatct ctccgcgtaa ttactgttac gcctgaatga
     1501 cttcattaca cctgatacag tgagttgaca ccgggcaaaa cgaccttaaa agtcctgtat
     1561 tttaccgtgg gcatcacccc agaattctga gctaaactgc tccagacggc gctaatgtac
     1621 gcctaaaact gaaacgagac aagaggcatt ttcttattta cttttagtaa cttacaggtg
     1681 tctcatatcg gcgacagttt aatttctgac ggtaataatt agagattcag cgaaggaaaa
     1741 aaccttaaga aaaaagccat aaaaaccatg aggttattat ggctgatttg aggagggaaa
     1801 gagtaagagc agtttgttaa atgtatagcg acgattctcc caccgggcgc gttttaaagc
     1861 gacggtggat ccagaggtac tgctccggtg cgcgcatgat ctctttttcg ataatcttgt
     1921 tcatataggc agcggcttga ttttcatctg ccgggtagcc ttccatctct ggggtgatgt
     1981 acaaacgata tccgctgtaa tctgcttttc ttaccatcgt tacggttaac atggctgcgc
     2041 cagagagacg tgaaaggaca tacgtaccat tggttgtggc gacattttcc accgcaaaga
     2101 acggcgcgaa ggagctgcct ttacgaccat aatcctgatc gggagcaaac cataccgctt
     2161 cacctttctt cagtgcaccg acaatgccgc gcagattatt tctgccgatc atcgctttgt
     2221 tagagcgcat gcgcccacgg gtctgcaccc attccatcag ctgattatta tgtggacgat
     2281 aggtagccat cattggttgg cacagtccca tcacgcggcc gcccagttcc agcgacataa
     2341 aatggacgcc gacaaccatt acgccgcgat tttgcatttg tgcgcgttta aggttatcca
     2401 acccttcaac atcaaaccat ttacgtacgc gactgtcggg ccagaaccac gccatgccgg
     2461 tttctaccag cgccatgccg agtgaacgaa agttttcggc aatcatcttc tcgcgttctt
     2521 ccgcagaatg ctgtgggaaa caaagttcca ggtttttacg ggcgatagat tcacgccgtt
     2581 tcaggaatgg tcgcgccatt gcgccaatac gcgtgccgag aaagcagaga acaggataag
     2641 gaagctgtac ccaaagccag agtacaccaa gcccaaacca tgtgagccag tagcgaggat
     2701 gtagaaactc gcgggaaaat ttgcattgtg gaaacatagt taccttctgt agatacgaat
     2761 tgttgtatcg cagaagattc catgtcgaca gcggtacact caattggttg ggccatcatg
     2821 tacgacaatg gttcctgtaa agagtgtaaa gactagtcag gacataacca cgaggagcat
     2881 gaatgcggtg gctctacttt atcactggct gactgctttc agctactgat tattaatcgt
     2941 gcaaagtaaa aaattttccg cacgcaatag cgttgcattt aacgtgctgt tttcattgat
     3001 gcttagcaaa aattgccaga ggttaaggat tgtcttacta ttaaagtgac tgaagttaca
     3061 ggttttttag cgcggcagga ggttgagcta tgatttattt atggatgttt ctcgccctgt
     3121 gtattgtctg cgtaagcgga tacatcggtc aggtactgaa cgtggtctcg gcggtctctt
     3181 ctttcttcgg catggtgatc ctcgctgcac tcatttacta cttcaccatg tggttaactg
     3241 gcggtaatga actagtgacg gggatattta tgtttcttgc cccggcttgt ggcttgatga
     3301 ttcgctttat ggtggggtat ggcaggcgct agtgggtcag gatggttcat ttgggcaggt
     3361 gttcacatca gcatcaggca tgattgttca cctgccaggt gacagacgct atcgcacagg
     3421 atgaataaga taattcgcct ttgtcgaaaa agggggcgta atgtcttctg tgagatttaa
     3481 tggtggcgca tcttctctca acttaaacaa aatcgcctgt gatggctgaa gttttgtaga
     3541 atgagttgtc gtcatcactg acgttttttc tgtctcttta tagctcgtct cacttctacc
     3601 accagaaatg gaaaatgcaa aaacattgca tgccataata gagaccaata aaaataagct
     3661 aaaaatcatt aagtttactt tcatagaaag ttatcctgaa a
//
LOCUS       DAZKGF010000290         3690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZKGF010000290 DAZKGF010000000
VERSION     DAZKGF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.290"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            482..2410
                     /gene="thrS"
                     /locus_tag="ACHOQV_004927"
     CDS             482..2410
                     /gene="thrS"
                     /locus_tag="ACHOQV_004927"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP9633161.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2414..2956
                     /gene="infC"
                     /locus_tag="ACHOQV_004928"
     CDS             2414..2956
                     /gene="infC"
                     /locus_tag="ACHOQV_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP9633162.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3053..3250
                     /gene="rpmI"
                     /locus_tag="ACHOQV_004929"
     CDS             3053..3250
                     /gene="rpmI"
                     /locus_tag="ACHOQV_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP9633163.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3304..3660
                     /gene="rplT"
                     /locus_tag="ACHOQV_004930"
     CDS             3304..3660
                     /gene="rplT"
                     /locus_tag="ACHOQV_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP9633164.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
BASE COUNT         1024 a          829 c          924 g          913 t
ORIGIN      
        1 tgattaatta ttatcgccag aacgaaatgt gcacgaaaaa ttgctataag aattgtgtca
       61 cttttatcat tgtgatatga ccacttcgtc attaatctta tgatcgttta aatagtaccg
      121 ccaaattacg caacgaactt tgctggattc agtttatgct gctgcaaatc cgctcgcagt
      181 aaacctttca gacgctccgt gatgttatca gtggttcttt aagcgttttg ctggtgtact
      241 cactacaaac gaattgcgaa tcgaatcaat gtgaaacgga aaggtacaat ctcccttttt
      301 ttagtgttaa catcgttcaa ccgggtggaa ggtgagaggt taatgtcttt catttgtgta
      361 cctgattgcg aaccaattta gcatttgttg gctagatggt ttcgcaatga actgttaata
      421 aacaaatttt tctttgtatg tgatctttcg tgtgggtcac cactgcaaat aaggatataa
      481 aatgcctgtt ataactcttc ctgatggcag ccaacgccat tacgaccacg ctgtaagccc
      541 catggatgtt gcgctggaca ttggtccagg tctggcgaaa gcctgtatcg cagggcgcgt
      601 taatggcgaa ctggttgatg cttgcgatct gattgaaaac gacgcacaac tgtcgatcat
      661 taccgccaaa gacgaagaag gtctggagat cattcgtcac tcctgtgcgc acctgttagg
      721 gcacgcgatt aaacaacttt ggccgcatac caaaatggca atcggcccgg ttattgacaa
      781 cggtttttat tacgacgttg atcttgaccg cacgttaacc caggaagatg tcgaagcact
      841 cgagaagcgg atgcatgagc ttgctgagaa aaactacgac gtcattaaga agaaagtcag
      901 ctggcacgaa gcgcgtgaaa ctttcgccaa ccgtggtgag agctacaaag tctccattct
      961 tgacgaaaac atcgcccatg atgacaagcc aggtctgtac ttccatgaag aatatgtcga
     1021 tatgtgccgc ggtccgcacg taccgaacat gcgtttctgc catcatttca aactaatgaa
     1081 aacggcaggg gcttactggc gtggcgacag caacaacaaa atgttgcaac gtatttacgg
     1141 tacggcgtgg gcagacaaaa aagcgcttaa cgcttacctg cagcgcctgg aagaagccgc
     1201 gaaacgcgac caccgtaaaa tcggtaaaca gctcgacctg taccatatgc aggaagaagc
     1261 accgggtatg gtattctggc acaacgatgg ctggaccatc ttccgtgaac tggaagtgtt
     1321 tgttcgttct aaactgaaag agtaccagta tcaggaagtt aaaggtccgt tcatgatgga
     1381 ccgtgtcctg tgggaaaaaa ccggtcactg ggacaactac aaagatgcaa tgttcaccac
     1441 gtcttctgag aaccgtgaat actgcattaa gccgatgaac tgcccgggtc acgtacaaat
     1501 tttcaaccag gggctgaagt cgtatcgcga tttgccgctg cgtatggccg agtttggtag
     1561 ctgccaccgt aacgagccgt caggttcgct gcatggcctg atgcgtgtac gtggttttac
     1621 ccaggatgac gcgcatatct tctgtactga agaacaaatt cgcgatgaag ttaacggatg
     1681 tatccgttta gtctatgata tgtacagcac ttttggcttc gagaagatcg tcgtcaaact
     1741 ctccactcgt cctgaaaaac gtattggcag cgacgaaatg tgggatcgtg ctgaggcgga
     1801 cctggcagtt gcgctggaag aaaacaacat cccgtttgaa tatcaactgg gtgaaggcgc
     1861 tttctacggt ccgaaaattg aatttaccct gtatgactgc ctcgatcgtg catggcagtg
     1921 cggtacagta cagctggact tctccttgcc gtctcgtctg agcgcctcct atgtgggcga
     1981 agacaacgag cgtaaggtac cggtaatgat tcaccgcgca attcttgggt cgatggaacg
     2041 tttcatcggt atcctgaccg aagaattcgc tggtttcttc ccgacctggc ttgcgccggt
     2101 tcaggttgtt atcatgaata ttaccgattc acagtctgat tacgttaacg aattgacgca
     2161 aaaactatca aatgcgggca ttcgtgttaa agcagacttg agaaatgaga agattggctt
     2221 taaaatccgc gagcacactt tgcgtcgcgt cccgtatatg ctggtctgtg gtgataaaga
     2281 ggtggaatca ggcaaagttg ccgttcgcac ccgccgtggt aaagacctgg gaagcatgga
     2341 cgtaaatgaa gtgatcgaga agctgcaaca agagattcgc agccgcagtc ttaaacaatt
     2401 ggaggaataa ggtattaaag gcggaaaacg agttcaaacg gcgcgcccta accgtatcaa
     2461 tggcgaaatt cgcgcccagg aagttcgctt aacaggtctg gaaggcgagc agcttggtat
     2521 tgtgagtctg agagaagctc tggagaaagc agaagaagcc ggagtagact tagtcgagat
     2581 cagccctaac gccgagccgc cggtttgtcg tataatggat tacggcaaat tcctctatga
     2641 aaagagcaag tcttctaagg aacagaagaa aaagcaaaaa gttatccagg ttaaggaaat
     2701 taaattccgt cctggtacag atgaaggcga ctatcaggta aaactccgca gcctgattcg
     2761 ctttctcgaa gagggtgata aagccaaaat cacgctgcgt ttccgcggtc gtgagatggc
     2821 gcaccagcaa atcggtatgg aagtgcttaa tcgcgtgaaa gacgatttgc aagaactggc
     2881 agtggtcgaa tccttcccaa cgaagatcga aggccgccag atgatcatgg tgctcgctcc
     2941 taagaagaaa cagtaaggcc ttcaagtagc actgtctgtg gagcctttgg gcttcacagg
     3001 cattgttcgc ctttgtttcg tttattaaca atgcgaagtg gaagttatta aaatgccaaa
     3061 aattaagacc gtacgcggtg ctgctaagcg cttcaaaaaa accggtaaag gtggttttaa
     3121 gcataagcac gctaacctgc gtcacattct gaccaaaaaa gcgaccaaac gtaaacgtca
     3181 cctgcgtccg aaagccatgg tttccaaagg cgatctgggc ctggtaatcg cgtgcctgcc
     3241 gtacgcataa gccgttaacg ttttttaact ttttaattag aatatagata caggagagca
     3301 catatggctc gcgtaaaacg tggtgttatt gcacgcgcac gtcacaagaa aattttgaaa
     3361 caagctaaag gctactacgg tgcgcgttct cgcgtatacc gcgttgcctt ccaggctgtt
     3421 atcaaagctg gtcagtatgc ttaccgtgac cgtcgtcaac gtaagcgtca gttccgtcaa
     3481 ctgtggattg cgcgtatcaa cgcagcagca cgtcagaacg gtatttctta cagcaaattc
     3541 atcaatggcc tgaaaaaagc ctctgttgaa atcgaccgta agatcctggc tgatatcgca
     3601 gtattcgaca aagtagcgtt caccgctctg gttgaaaaag cgaaagcagc tctggcataa
     3661 gccagttgaa agagggagct agtctccctc
//
LOCUS       DAZKGF010000291         3679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZKGF010000291 DAZKGF010000000
VERSION     DAZKGF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.291"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2479)
                     /locus_tag="ACHOQV_004931"
     CDS             complement(<1..2479)
                     /locus_tag="ACHOQV_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP9633165.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREIIKRLGINTDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNSNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSIQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPFRGAVVL
                     VNFDTDQRKPWFIKALRTDGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCAITFGKEIDESRNYIFQ"
     gene            complement(2495..3169)
                     /locus_tag="ACHOQV_004932"
     CDS             complement(2495..3169)
                     /locus_tag="ACHOQV_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9633166.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSENWVTISDVKANNVKVNYETIMIAPLESQSINVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(3250..>3679)
                     /locus_tag="ACHOQV_004933"
     CDS             complement(3250..>3679)
                     /locus_tag="ACHOQV_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9633167.1"
                     /translation="SPCKFEAGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSML
                     KISCPKEVAGVKLSLITNDKITGNDKAIASSNDTVGYYLYLGDNSDVLDISAPFNIES
                     YKTAEGQYAIPFKAKYLKLTDNSVQSGDVLSSLVMRVAQD"
BASE COUNT         1004 a          910 c          695 g         1070 t
ORIGIN      
        1 actggaaaat ataatttcta ctttcatcaa tctctttacc gaaggtgatt gcgcatgaaa
       61 gcccttgttg cttatcaatt gccacattaa ccgatggcgg tacttcattg gtgcgaataa
      121 ataactggct tccctggccg acaacaccaa tattatgacc atggatatca ttgacttcat
      181 aaccaaacgt taatggttgc ccatccgttc ttaacgcttt tataaaccag ggcttgcgct
      241 gatcggtatc aaaattaacc agtacaaccg cgccgcgaaa aggggcggca attttccggt
      301 tgccacgtaa ttctgcttcg ctatcgcttt gcgacacatc cagcatcagg tgattttccc
      361 gataaggtgt cattccgtcg tataccacca ctccattacg gtttgttgtg cgatattttt
      421 gcccattgac ataagcatct ttaattcctg gcgcattcat tacagcaaac gtttcggaaa
      481 gacggttcgc cagattaacg ccacccgacc aggcgacaat gccccctgaa acactggctc
      541 cagcctgtcg ataagtactc gactgactat aactgccatt caccgttgcc accggcgcgt
      601 tccaggttaa attcgctcca gctgtcgttt catttccctg atgctgataa ctcaggttga
      661 ccccatagtt aaactgatcc cggcttccaa cggttcctga taatcccgta ttatttgagg
      721 caaccccctg atcatcaaac gtcgttgagt tagacatata tatttgccga cgaggcgtcg
      781 taacgtcatc accccaatca aagggaatcg atataaaaat attaaaacgt ttctcttcat
      841 gatgattctc gtcataagca tggcttgccg cgagggtata gcttatcctt cgcaggttgt
      901 tggaataact caactgataa tccttgctgc tgccgctacg cccccagtaa tctcgccata
      961 acgtacttaa ggacacagaa ccccagcctt ctggcaatga ctggctcata ttggcagaaa
     1021 agctattttt acggccaaaa tcgttctggt aataatcggc aatgtcatag atatcgtttt
     1081 catcacggcg ataattatct ttattgtttg cccatacgtg atcattaaat gtccgataat
     1141 cacgcgacga ataacgccag gccgccagac caaaacgtgt cgacgtttgg ctcacaaatt
     1201 tgttgtaggc aatttgataa ctttgcccgt caaacacatc gccgttgtct tgtatactat
     1261 gcgacttcgt ggcatcgacg gaaatggcac caatgcgtgt attccagcca gtccccaaag
     1321 tgaacgcgta ataattattc gcgaccatcg atccgccata cagcgtcaat aagttattaa
     1381 aaccatactg ataacccgcc tgaacaaaat cactttgttt gctcgcccct tcaatatggc
     1441 tacgacctgc cgcaaaatca tatttcgaca cgccgggttg cagcatattc ggcactgctg
     1501 cataaggcac cagataggtg gttaccgaac cgtccgcctc tttcacgctg acatcaagat
     1561 ctgctccacc accggccaac tgcaaatctg taatcgcaaa cgggccagga ggaacctctt
     1621 tctgataaac cacaaaacca ttctgttcaa tagttaccag cgcgttactc tgagcaatcc
     1681 cctgcacccg tggagtaaaa ttttgtttcg agttaggcaa catctgcata tcacgaaaca
     1741 accgcacacc gctgaagcga acagaatcaa aaatatcgct tgatgtgtac atatcaccca
     1801 cgcgaagcgt gccgagaagt tgggcaaatc cacgttccag atacagggta ttgcttttcc
     1861 acacccctgg attgctattt gttttactga aactggcatc agaatgcagt tgccacccca
     1921 gtaagtttaa cccgctgtta aaacgtacat atgtactctt gctattaccc gacgctttat
     1981 agtcgctgta atactgactc acataataag aggtataaaa cgcattaata ccccgctccc
     2041 agttttccgg tggaacatag ccactttcca gttcttccac ccacgcctgc gggacactga
     2101 aatcgagacg aaaaactccg atatcccagg tatagctccc accctgaaca agttgctcaa
     2161 atgttaaaca ttgcttaccg ctggcaaagt tatcggtatt aatgcctaac cgcttgataa
     2221 tttcccttga taaacatgtt tcttgcgggt tgtctttaac aataatctca tatttcccgc
     2281 gccattgctt attgacataa atatcgatgt catactgccc cggcaagggt tgattatcat
     2341 caagacgaat atttgcaacc tgctggtctt tcattccacc tatcatgaaa tgggtatcaa
     2401 aggtttcttc agctgcataa gcttcaatgc cgagcaataa cgctacgatt gctgatgcaa
     2461 gtggggtcat tctcaacata ggcagctcct gcaattaaat tttgtcacta atatagttgc
     2521 catggtcatc gataatggtc agatgccagt tattcgcatt attacttttg acattaatac
     2581 tctgactttc taagggggca atcataatag tttcataatt gactttgaca ttattagctt
     2641 tgacatccga aatcgttacc caattttctg agtcattttt tatcaccaat ccattgccac
     2701 tgcgatttac cagtaacttt ttaaatgtcg ctttatttac cggagcaata cccgctggcc
     2761 ggtaaaacaa cttaattctg ttttgcatcg caaacttcag tgcattctta ccttcttgtt
     2821 ctggactatt tggtggaata tccagaacat tcagataaaa aatactctct ttattagtgg
     2881 gtagtttatt cggcataatt ttaattttta cttgctgccc ggaatttgcc cctatttttg
     2941 ccactggtgg cgttaacatg aaaggaacct gaattttttc tggtggtaat gacgtatcgc
     3001 catcatcaat ccacgcctgc agcagcgaag aacggtttcc ctggttcatc aactgcacca
     3061 tcacttcttt attttctgcc gggtaaataa tgcgcgtccc gtagataaca ataccggcat
     3121 gtgcaggaag gaccatagaa gaaatgagta aagctaataa tcctttcatc ttaatgcctc
     3181 ttaaattaaa aggccgccat ggggatagcg gccatgttta ttacctggag aaacttgcaa
     3241 ttttatttat taatcctgcg ccacacgcat aaccagagaa gataacacat cacctgattg
     3301 cactgaatta tctgtcagct tcaggtattt tgctttaaac ggaatagcat attgaccttc
     3361 cgctgtttta taactctcaa tgttaaaagg tgcagaaata tccaggacat cactgttatc
     3421 acctaaatag agatagtaac ccacggtatc gttgctactg gctatcgcct tatcgttgcc
     3481 ggttattttg tcgtttgtga tcaacgagag ttttacacca gcaacttctt tcgggcagct
     3541 aatttttaac atgctgctgg ttacaccgac tgcatcatca taggtagaat atttagcttt
     3601 accttcaaag acagtggttg gtacagttgg catattaata cttactgaat cgccacctgc
     3661 ttcgaattta cacggagat
//
LOCUS       DAZKGF010000292         3679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZKGF010000292 DAZKGF010000000
VERSION     DAZKGF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.292"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(423..866)
                     /locus_tag="ACHOQV_004934"
     CDS             complement(423..866)
                     /locus_tag="ACHOQV_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633168.1"
                     /translation="MHHYKFFALIAMLLCGASFASDVYFLCNTEKGTIKLDENKGVLR
                     YTLSKDHKTEFNYESKGSDYSGFKYNHYSRFQTDYFNVSFVNSEYKYTIFSNYEDDRK
                     SSGVSVTSLNSKKESVYYCKSIGIDRLSDLSAKMACDKYSALGCD"
     gene            complement(853..2997)
                     /locus_tag="ACHOQV_004935"
     CDS             complement(853..2997)
                     /locus_tag="ACHOQV_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase family protein"
                     /protein_id="HFP9633169.1"
                     /translation="MLNQETPEKSVPLQFMMDGEVVAWRVNRDYAAIECYQERPLRQS
                     GTFVLVKSVYKPDEQDESSWLTLYQLYMHIAPLSEFPKRPLYRVTQKGHGVRMRKYSR
                     HDDSREIAPDVLANKHGHPRTLTQGDTLAVLQQKSFLLKQRPESFALVQRLQDGKPAG
                     ELFWVSMRPEYLEPDGQCYVCLPDWMHSALNHGVFDDVVVPPVPLKVMVKAGDAVGFL
                     GAQDLADEDNYPQIITTDYKAHIELLSLDEHVPDVVANMKGIKTGKQFIKLKLKRPLY
                     LRNGEGEESTFEQMSAITRIDAGKIIPRDATYPFTDKTGITYFQIRPHTWMHQNDVEQ
                     LSQHDLAGLNFHCIEAEHTTDFTRTLDERWLIDALKSISSHFDGEKGPQFAQAKMFYD
                     SLIHNAENRRAPNPYPDKSLDQYLFGALHTNQMNIPEYARRLIVKHDSCWHSTRDDTR
                     WSSVCKSRDESPVVKMANGGFLDATHWMDKVPPFASQRSVWHFHPLEFPEILRSAEFP
                     RTPINGVLTPIEFIHFYNGDKIDDTDYEEAAKELGCEVAAIKAVAKTETGSYGSYFKF
                     EDNDDYVPAILFERHHFHKYTNGKYDQFEDISNPVAGGYGATSIQYAKLVKAYTLDKK
                     AALKSASWGKFQILASNYATAGYASPEDFVFALSKSEKNQLKAFVSFIKADRVLLRSI
                     RTKDWLSFAQRYNGPRQKGYDLKMERNYNASL"
     gene            complement(3506..3669)
                     /locus_tag="ACHOQV_004936"
                     /pseudo
     CDS             complement(3506..3669)
                     /locus_tag="ACHOQV_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742560.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          955 a          876 c          752 g         1096 t
ORIGIN      
        1 ttctggggat tcctcaggga gtgaccatag attccgtttt ccaggtgccc ccctttttct
       61 ccaaaaattg aaaaataact ctgtagatgc cgctaacaat caattcgagt tgatatttta
      121 aagcgttctg tgcagaaaga aataaatttc aaaaatgaat caggggagaa aagtgcgctt
      181 tttaacctgt tcagtgtcca gaacacatga aaccgatcgc cattcagttt catgcatagt
      241 catgcaacgc actgaaaatg attctgacgc atttttccgg tttcccccga ggaaaacatc
      301 cctttttgcg tcagaatcgc gctcagcgcg ttttagcggc acgtacaatt acaggattat
      361 aaatcgtcac cagcgactgg cgtaaaaaag cccctctctg caggcgagat ttagatgttt
      421 tattaatcac accccaaagc tgaatattta tcacaagcca tttttgccga caaatcacta
      481 aggcgatcaa tgccaatgga cttacaataa taaacagatt cttttttgct attcaggctc
      541 gtaacactaa ctccgctgct ttttctatca tcctcataat tactaaatat cgtgtactta
      601 tactctgagt ttacaaacga tacattgaaa taatcggttt gaaatcgtga gtaatggttg
      661 tatttaaacc ctgaataatc actacctttc gattcataat taaattcagt tttgtggtct
      721 ttcgataagg tgtaccgaag tacacctttg ttttcatcta gtttgattgt tcctttttcc
      781 gtgttgcata aaaaatacac atcacttgca aacgatgccc cacacagaag cattgcaatc
      841 aacgcaaaga acttataatg atgcattgta atttctctcc attttcaaat catatccttt
      901 ctgccttggt ccgttatatc tttgagcaaa tgaaagccaa tctttagtcc gaatagagcg
      961 cagtaatact ctatcagcct taatgaagct aacgaatgct tttaactgat ttttctcaga
     1021 cttacttaat gcaaaaacga aatcctcagg gctcgcgtag cctgctgtgg cgtagttcga
     1081 cgctaaaatc tgaaacttac cccaggatgc tgattttaaa gctgctttct tgtctaaagt
     1141 atacgcttta actagctttg catactgtat tgatgttgca ccatacccac ccgcaacagg
     1201 gtttgatata tcttcaaact gatcatactt cccatttgta tatttatgaa aatgatgcct
     1261 ttcaaataat atcgctggaa cataatcatc gttatcttca aatttaaaat aggagccata
     1321 agaaccagtt tctgtttttg ctacagcttt aattgctgca acttcgcatc ctaattcttt
     1381 tgctgcctct tcataatcag tatcatcaat tttgtctcca ttatagaagt gaataaattc
     1441 tataggagtt aatactccat taataggagt tctgggaaac tctgcacttc gtaatatttc
     1501 cggaaactca agcggatgaa aatgccatac agaccgctga ctggcgaacg gaggcacctt
     1561 atccatccag tgtgttgcat ccagaaatcc cccgttagcc atcttcacaa ccgggctttc
     1621 atccctggac ttacataccg atgaccagcg tgtatcatcg cgggtactgt gccagcagct
     1681 gtcgtgtttc actatcaggc gtcgcgcata ttccgggatg ttcatctgat tggtatgcag
     1741 cgcaccaaac aaatactggt ccagagattt atcgggatac gggtttggcg ctcttctgtt
     1801 ttcagcgttg tggatcaggc tgtcataaaa catttttgcc tgcgcgaact gcggtccttt
     1861 ttcaccgtca aagtgcgagc tgatgctttt cagggcatca atgagccagc gttcatccag
     1921 cgtgcgggta aaatctgttg tgtgttcagc ttcaatacag tgaaaattta gccctgctaa
     1981 atcgtgctga ctgagctgct ccacatcgtt ctggtgcatc cacgtatgtg ggcgaatctg
     2041 aaagtatgtg atcccggttt tatccgtaaa cgggtacgtg gcatcacgtg ggataatttt
     2101 tccggcatca attcgggtta tggcgctcat ttgttcaaat gtactttctt caccctctcc
     2161 atttcgcagg tacagcgggc gtttcagctt cagcttaatg aactgtttgc ctgttttaat
     2221 ccctttcatg tttgccacca catctggcac atgctcatcg agactcagta gctcgatatg
     2281 ggctttatag tccgtggtaa taatttgcgg atagttatct tcgtcagcca gatcctgagc
     2341 gccgaggaat ccgaccgcat cccctgcttt caccatcact ttcagtggca caggcggcac
     2401 caccacatca tcaaacacgc cgtgattcag cgcactgtgc atccagtcag gcaggcacac
     2461 gtaacattgg ccatcaggtt ccagatattc cggacgcatc gagacccaga aaagttctcc
     2521 ggcgggttta ccgtcctgaa gacgttgcac cagagcaaac gactccggcc gctgtttcag
     2581 taaaaacgat ttttgttgca gtacggcaag cgtgtcaccc tgcgtcagtg ttctggggtg
     2641 gccgtgtttg tttgccagta cgtccggcgc gatttcacgg ctgtcatcat ggcgggaata
     2701 tttacgcatc cgcacaccgt ggcctttttg tgtaacccgg taaagcgggc gtttcggaaa
     2761 ttctgacaat ggcgcgatgt gcatgtaaag ctggtacagc gtcagccagg atgattcatc
     2821 ctgctcgtct ggcttgtaca ctgattttac cagcacaaag gtaccggact ggcgcagggg
     2881 gcgttcctga taacattcga tagccgcgta atcgcggtta acccgccatg ccaccacttc
     2941 gccatccatc atgaactgga gcggcaccga tttttcaggt gtttcctgat tcagcaccga
     3001 ggccggggac gatgacctcc cgacatgaat acctccgtgc cagtaacgat ttacgccaag
     3061 tagccaccaa ccgtgggcgt ttttgcggat gtcggttagc agctcgtcat agctcttata
     3121 gtctcgtcca tcggctttcc gtaccggata acgtatgttc atcatctttt cttaagtgaa
     3181 atcacatatt tcgatggatt ctcgcgcgta ttttgggttt ggggtgtagt ggaaccaaaa
     3241 agacggttaa gcggttaaat tgaatggtca ataacaggta aatcaatctg ttgagatcaa
     3301 cgacgtcttt tgctccgcaa acggctaaga tgagtacaga taatttggtt atcgtaaaaa
     3361 aggtggctaa aaatttcaac ctgcaatgta aattggccgg ttgatttttg gctatttaaa
     3421 ttgacctctt aacggcgttt attgttccat tgctccccaa ccccctatgg attataagcc
     3481 gcacatacat tacttcgttc tgcatatcac cactaatctg cttaagatgt actaacaaca
     3541 tcatcgcctg ttggctaaac ggaactataa ctcctctttc attttcatgc cgcgatacaa
     3601 gtaatgcaca cctttaattt cttcacgttt tgccggaatg cgccaaatag cgttatcaaa
     3661 atcaaattct ttttaacga
//
LOCUS       DAZKGF010000293         3661 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZKGF010000293 DAZKGF010000000
VERSION     DAZKGF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.293"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..218
                     /locus_tag="ACHOQV_004937"
     CDS             <1..218
                     /locus_tag="ACHOQV_004937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633170.1"
                     /translation="KNNTSKPPDQQVSHRLSEQQNRFAGGWSHCVTPTTITSVFSRTV
                     VNHKIGTHAGGAGPHDFLFDESENGER"
     gene            509..703
                     /locus_tag="ACHOQV_004938"
     CDS             509..703
                     /locus_tag="ACHOQV_004938"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFP9633171.1"
                     /translation="MNQDKAIYLENKYFLVANEALRLERENNYLAAWIKWSEALYYAR
                     GKNISWCIARAEYCKKTGRF"
     gene            complement(2344..3342)
                     /locus_tag="ACHOQV_004939"
     CDS             complement(2344..3342)
                     /locus_tag="ACHOQV_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFP9633172.1"
                     /translation="MKNPELKNLTDYSPEDKPWDERRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVTEHPGARWLFLTLTVRNCSIDALGETLTAMNAGFQRLKDRKEFRHVLGWVRTTEVT
                     RGKDGSAHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRK
                     PKDDESLPCVVAEQLRGAVVETLKYSIKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRRLSSDD"
BASE COUNT         1036 a          918 c          808 g          899 t
ORIGIN      
        1 aaaaaaacaa cacatccaaa ccacctgatc agcaggtttc tcaccgactt tctgaacaac
       61 aaaaccgctt tgcaggtggt tggtcccact gcgtgacacc aaccaccatc accagtgttt
      121 tttctcgtac cgtcgtcaat cacaaaatcg gaacccacgc cggaggcgcg ggaccccacg
      181 attttctgtt tgacgagtca gaaaacggcg aacgctgaca gatttcaatc tggctgcgtg
      241 agacgttggc ggggggtcgg ggcgcagtcc tgaccaggcg gcatttgtct gattgcgtgt
      301 gcgcggtccg acaaatacac atcctgtccc gtttttcgca cggtttttat tgattaccgg
      361 acagatgaac ccggattgcc atgttgcgaa aatcacgtta aaacgattac gagcgtgcag
      421 ttgggctgat atatcaacag tcatgccaat atgtattaac ccgacaggcc gctctttttt
      481 aattcaaata aaaatataaa ttacgagtat gaatcaggac aaagcaattt atttagaaaa
      541 taaatatttt ctggtcgcaa atgaagctct tcggctggag cgtgaaaata attaccttgc
      601 tgcatggata aaatggagcg aggcgctcta ttacgccaga ggcaaaaata taagttggtg
      661 tatagccaga gctgaatatt gtaaaaagac aggccggttt taattctgac ggggtttcaa
      721 agggggcgtc ccctttggca cgtcgacgta tcaaaatgcg ccagcatttt gtatacagtc
      781 ggtaacgtgc ctccaggatt taccatcaaa aacgcccctg tagttaatcc tgaatcaaga
      841 tataactaag atataattag cgtataacta aggcataact aaagtataac taacttagtg
      901 tgcatataat taagatataa ctaatatata actaatgtat aactaacggt tcaccaacgg
      961 atcgcatgta gtagacgcta cataaaaaat cctgaaatgg aggattcgtc ataaagagat
     1021 tcctgttttt agcgttctga tgcaggatag ggcaaacacc gcaaaatgac gtctttggta
     1081 tgttttgtga tgtttgcttc tggcatctgt tatctgtttt attgggtgtt tttatgagtg
     1141 ttgatgtgat taaacaaatt cagagtatac aggacaaagg aattattatt tattcaaaat
     1201 acagagctaa cgaatttaac agggatgatg tatacagaga gagttacagc ctgattattg
     1261 agtttaatca tcttatttct gaacatgccg ttcatgatga aaaactgatt aaccatactg
     1321 tctgtattct gaatgaatta cgaagcatag cgataaatta aaaaatcccc ctggattaca
     1381 gggggaagcc atcagagttt gagtaatgag aaatattatt ctggagagag caccacaaat
     1441 gcgctctttt ccaatctgcc gtaaccatca gcaaaagaca atcagaacag gcgaaaaaat
     1501 ccccctatat ggcaaggggg tgtcaatgag gacatggcaa ttgtgagttt tgtgtgtgtt
     1561 ttatgtgttg cagtttgcaa cgtcagaaat ttgccgtagt tcgtcatgag agacaacaaa
     1621 aaaatccctt ggaactggtg gaggccagac caaggggtag caaatggcta attaattctc
     1681 gtagttttga tatttccatt gttgcaatcg caacggggtg aatttgccgc agttgtgcgc
     1741 gaaaaacaat cctgattccg ggacaggatg tgtaatcgta taaccgcgca cgcacggcta
     1801 tacgattacc acctggtcag ggctttgccc cgacaccctc gacaccgttc ccgcgatacg
     1861 atagaacatc agggcgcgtt tccggtaatc aactgaccat ggggatggtt tgcattagca
     1921 acaggtgccg gaaccgcgca caaaatcatc agagacgcgg cgaacaaaaa acaaacaaaa
     1981 acattgtcag ctgcaaaaaa tcgagttaaa acaactgcga gcgccttcct gtctccttca
     2041 attacggtcg tacattcatt gcatctgtcg gggaggcgct cacctccata atccgatttc
     2101 tccattgaca ataattccgg tgataccccc tcttttgagg ggcttttttt acccacacac
     2161 cacaaacagc agccatatca tcgtcaaaaa cgcgctcaaa acgtctctga cggcctgaac
     2221 ccatcaaaac atacaaaacc attgcccaat gcaaaaaact gcgttagaaa gcgtctgagc
     2281 gcgtttctgg tgagcaaaaa gctgtcagcc gtggatttcc attgcagaga cccagaaacg
     2341 cgctcaatca tcgctgctta gccgacgttt atctttcgcc cgatcacgct ggtatttttt
     2401 gacaaccggc acccacgaaa acgccagacg ggaaccgtca tctgtaccct ctgccatgcc
     2461 atccgccatg attaaatcct cgttcgtttc ccgctccagt tgcagaacgt tcttaagcgc
     2521 tccccccgta gcaacaaaac gacgcttgtg tacctgccgt gttaattcga ggaaccattc
     2581 aggatcagcc agcatatcag caggctttat ggaatatttc agagtttcaa ccaccgcgcc
     2641 gcgcagctgt tccgcaacaa cacaaggtaa agattcatcg tcttttggtt tccgtgactt
     2701 caccactctg atatccacat tcgggtcata cgccacccgc agacaatcac gccacagttc
     2761 cacccaacgg gcatgtttca catactcacg gccggacaac atcgaggggg gaaccatcag
     2821 cagagtgtga aaatggggat gtgcgctacc gtccttgccg cgcgttactt ccgtggtacg
     2881 cacccatccc agcacatgac gaaattcctt gcgatctttc agccgctgaa aaccagcatt
     2941 catcgcggtc agcgtctcac caagcgcgtc aatggaacag ttgcgcaccg tcagcgtgag
     3001 aaacaaccag cgcgcgccgg gatgttccgt cacaatcttt ggcagggact gatagaaacg
     3061 ggcttgccac atcatggagc gcctccactg gcagatcgga cagtgccgga cacgacagaa
     3121 ccgcgcatca cgcaaccgca ggcgcgtttc tccggtctct gtgtttgttg accacccaaa
     3181 ccgcaaaata ccactacaag ccccgacacg ttcgccgtag tgctcaaatc cggcagcacg
     3241 cagataaata ccgcccacat cgtcggaaaa gctccggcgc tcatcccacg gtttatcctc
     3301 cggggaataa tccgtcagat ttttaagttc aggatttttc atgtctgccg ttccctagtg
     3361 agcgacagac gaaattacgt gttatcattc ctgtatcacc tgtgttgtcg tgtcaccaat
     3421 tcgaattcct acaggtggtc acacccccga acgacgccca tcgttgcggg ggtcgctttt
     3481 aatgcgacca cttccgacaa aatactctca aacctatttc cacgcaaatc cgcgctcagc
     3541 ccagatgtga caagggctag agctaaatta caatcccaga gatatccctt aagtataaat
     3601 acaaggatcg cgcgcgcgat cgcccaccca aaatgcgcgt tttttgagcg ttcccgagaa
     3661 c
//
LOCUS       DAZKGF010000294         3656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZKGF010000294 DAZKGF010000000
VERSION     DAZKGF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.294"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            53..1102
                     /gene="allD"
                     /locus_tag="ACHOQV_004940"
     CDS             53..1102
                     /gene="allD"
                     /locus_tag="ACHOQV_004940"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFP9633173.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVNALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1124..2359
                     /gene="allC"
                     /locus_tag="ACHOQV_004941"
     CDS             1124..2359
                     /gene="allC"
                     /locus_tag="ACHOQV_004941"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFP9633174.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKQNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2370..3155
                     /gene="allE"
                     /locus_tag="ACHOQV_004942"
     CDS             2370..3155
                     /gene="allE"
                     /locus_tag="ACHOQV_004942"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFP9633175.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELEHIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3284..>3656)
                     /locus_tag="ACHOQV_004943"
     CDS             complement(3284..>3656)
                     /locus_tag="ACHOQV_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9633176.1"
                     /translation="VFLNADIKPGIEIVLNAVNLAQAVQGAALVITGEGRIDSQTAGG
                     KAPLGVASVAKQFNVPVIGIAGVLGDGVEVVHQYGIDAVFSILPRLAPLAEVLASGET
                     NLFNSARNIACAIKIGQGIKN"
BASE COUNT          920 a          898 c          945 g          893 t
ORIGIN      
        1 cctgtatgtt aattgcagtc atgcttcaca ccgtcgttaa aaaggaagac agatgaaaat
       61 cagtcgggaa acactccacc agctaattga gaataaactc tgccaggctg ggttaaaacg
      121 tgagcacgct gcaaccgtgg ctgaagtatt ggtttacgcc gatgccagag ggatccactc
      181 tcatggcgcg gtgcgcgtgg aatactacgc ggaacgcatt tcaaaaggcg gcaccaaccg
      241 cgaaccggag tttcgtcttg aggaaaccgg gccgtgctcg gcaattttac atgccgacaa
      301 tgccgccgga caggtcgcgg cgaaaatggg tatggaacat gccatcaaaa ccgcccagca
      361 aaatggcgtt gcggtggtcg gtatcagccg gatgggtcac agcggcgcaa tctcttattt
      421 tgtgcagcag gcagctcgcg ccgggttaat tggcatttcg atgtgccagt ccgatccaat
      481 ggtggtgccg tttggcggcg cggaaattta ctacggtact aacccactgg cctttgccgc
      541 gccgggagaa ggcgacgaga tccttacctt tgatatggcg actaccgtac aggcatgggg
      601 aaaagtgctc gacgcccgct cgcgtaatat gtctatcccg gatacctggg cggtcgataa
      661 aaacggagca ccaacaaccg atccatttgc agttaacgcc ctgctccctg ccgccggacc
      721 gaaagggtat ggcctgatga tgatgattga cgtcctctca ggcgtcttac tcggcttacc
      781 gttcgggcga caggttagtt cgatgtatga cgatttacac gccgggcgta atttggggca
      841 attacatatc gttattaatc cgaacttttt ctcctccagc gaattattcc gtcaacatct
      901 tagccagacc atgcgcgaat taaatgccat tacccccgcg cccggtttta atcaggttta
      961 ttatcccgga caggatcagg atattaaaca acgcaaagcc gccgtcgaag gcatcgaaat
     1021 tgttgatgat atttaccagt atttaatttc cgacgcgctt tataacacgt catacgaaac
     1081 gaaaaatccc tttgcgcaat aattattgag acaggacttt cttatgatta cacatttccg
     1141 tcaagccata gaagaaacgc tgccctggct ttcctctttt ggcgctgacc cagcgggtgg
     1201 gatgacccgt ttactttatt cgccggaatg gctggaaact cagcagcagt ttaaaaaaag
     1261 aatggcagca agcgggctgg aaacacgttt cgatgaagtg gggaatttat acggtcgcct
     1321 gagtggcacc gaatatccac aggaagtggt tctgagcggt tcgcatatcg ataccgtggt
     1381 taacggcggt aacctcgacg ggcaattcgg tgcgctggcg gcgtggctgg caattgactg
     1441 gctgaaaacg caatacggcg cgccgttacg tacggtcgaa gtggtggcga tggcagaaga
     1501 agaaggcagc cgcttcccgt atgtcttctg gggcagtaaa aatatctttg ggctggcgaa
     1561 tcctgacgac gtgcggaata tctgtgatgc caaaggaaat agttttgtcg atgcgatgaa
     1621 ggcttgcgga tttactctgc cgaacgcccc actaactccg cgtcaggata ttaaagcctt
     1681 tgtcgaactg catatcgaac agggctgtgt gctggaaagt aatgggcagt caattggcgt
     1741 ggtgaatgca attgtcgggc agcgtcgtta tacggtgacg ctgaacggcg aatcaaacca
     1801 tgcaggcacc acgccgatgg gttatcgtcg tgatacggtt tacgctttca gtcgcatttg
     1861 ccatcagtcg atcgaaaaag cgaaaaagat gggcgacccg ctggtgctta cctttggcaa
     1921 ggtggagccg cgtccgaata cggtgaatgt ggtaccgggt aaaaccacgt tcaccattga
     1981 ttgtcgtcat accgacgctg ccgtgctgcg cgatttcacc cagcagttag aaaacgacat
     2041 gcgggcgatt tgcgatgaaa tggacattgg tattgatatc gatttatgga tggacgaaga
     2101 acccgtgccg atgaataagg agctggtcgc caccctgaca gaattgtgtg aaagtgaaaa
     2161 acagaactac cgggtaatgc acagtggtgc cgggcacgac gcgcaaattt tcgcgcctcg
     2221 cgtgccgacc tgcatgattt tcattcccag catcaacggg atcagccata acccggcgga
     2281 acgcaccaat attaccgacc ttgccgaagg ggtcaaaacg ttggcactca tgctttatca
     2341 acttgcctgg cagaaataag gagttataaa tgggatattt aaataacgtc accggttacc
     2401 gcgatgattt actggctaac cgtgcgattg tgaaacacgg taatttcgca ctgttaacac
     2461 cagacggtct ggtaaaaaat attattccgg gctttgaaaa ttgtgacgcg acaatcctct
     2521 ccacgccaaa gctgggtgcc tcttttgttg attatctggt cacactgcat caaaacggcg
     2581 gcaaccaaca gggcttcggt ggcgaaggca ttgaaacgtt cctgtatgtg atctctggaa
     2641 atatcactgc caaagccgaa gggaaaacat ttgccttaag cgaaggtggc tatctttatt
     2701 gcccgccagg ctccttaatg acgtttgtta acgcccaggc cgaagacagc caaatctttt
     2761 tatataagcg tcgctatatt ccggtagaag gtcatgcgcc gtggctggtt tctggcaatg
     2821 ccagcgaact ggaacacatt cattatgaag gcatggacga tgttattctg ctggattttc
     2881 tgcccaaaga gttaggtttt gatatgaaca tgcatatcct ctcttttgcg ccaggtgcca
     2941 gccacggtta tatcgaaact cacgtccagg aacacggtgc ctatattctt tccggtcagg
     3001 gggtttataa cctcgacaat aactggatcc cggtgaaaaa aggcgattac atctttatgg
     3061 gcgcttactc cttacaggcc ggttatggcg tagggcgtgg tgaagcgttt agttatattt
     3121 actcaaaaga ttgcaaccgc gacgtagaga tttaagtcat accactcgcc tgattatttt
     3181 tagctatgtt ggattttgcc acggtttatt ccgtggcttt ttttgtagtc aattcaggta
     3241 aaaaaagatg cgatataaat cgcatccgct ttaaaggtaa gagtcagttt ttaattccct
     3301 gacctatttt aatggcgcag gcaatatttc gcgcgctgtt aaagagattg gtttcaccgc
     3361 tggcgagcac ttcggctaga ggtgccagac gtggcaaaat gctgaatacc gcgtcaatgc
     3421 cgtactggtg caccacttcc acgccatcac ccaatacgcc agcaatccca atcaccggga
     3481 cattaaactg cttcgccacc gacgccacgc ccagcggcgc tttaccacct gccgtttgcg
     3541 agtcgatgcg cccttccccg gtgatcacca gtgctgcgcc ctgcactgcc tgcgcaagat
     3601 tgaccgcatt caacacaatc tcaatacccg gtttaatatc cgcattgagg aatacc
//
LOCUS       DAZKGF010000295         3584 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZKGF010000295 DAZKGF010000000
VERSION     DAZKGF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.295"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..141
                     /gene="ymgI"
                     /locus_tag="ACHOQV_004944"
     CDS             <1..141
                     /gene="ymgI"
                     /locus_tag="ACHOQV_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFP9633177.1"
                     /translation="PVKNIIPIITVTLILNYLNNSERSLVKQILIEDEIIVCATYLIP
                     DI"
     gene            143..487
                     /gene="ymgG"
                     /locus_tag="ACHOQV_004945"
     CDS             143..487
                     /gene="ymgG"
                     /locus_tag="ACHOQV_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFP9633178.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            491..826
                     /gene="ymgD"
                     /locus_tag="ACHOQV_004946"
     CDS             491..826
                     /gene="ymgD"
                     /locus_tag="ACHOQV_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFP9633179.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(884..3526)
                     /locus_tag="ACHOQV_004947"
                     /pseudo
     CDS             complement(884..3526)
                     /locus_tag="ACHOQV_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
BASE COUNT          937 a          864 c          782 g         1001 t
ORIGIN      
        1 cccgtaaaaa acataattcc tatcatcaca gtcacgctca ttttgaatta tctgaataat
       61 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
      121 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
      181 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
      241 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
      301 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
      361 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
      421 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
      481 gaaataagga atgatgatga aaaaatttgc tttactggcg ggtctgtttg ttttcgcacc
      541 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
      601 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
      661 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
      721 aagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
      781 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtta
      841 ccgcgtcgat gaattcgacg cggtttttta gctaatggcg ggatcagaac ttatatttca
      901 ggcccaccat cattgcggtg tcattgtagc cattatcccc cagctgcacg cccacattac
      961 cccagacgct ggccgccgga ttcagattgc catttacgcc aagtttaatt tcccccagat
     1021 tacgcgcccc gtcttgttca actttcacac cgttcattga gacagcaaaa tctttactgt
     1081 tatggatcca gttcatttca acaaaaggtt caaagtcgtc accgtgcgga ccgctgtttt
     1141 tctcctgagt acgaataaag gtgcggaagc cgagtcgggt ctggatgttg ttatttgcgt
     1201 cattctcaat acgtgagcca ttggcttccg tgtgcgcatt atgatcgacg cccatccaga
     1261 tagcctgtgg ctggaagagc cacgtatatt tcgccgcttc cagaccaaag gatttattca
     1321 gggcaaaacc gtaacccgct tccagcgaga cggttgcacc tttcgcgcta taggattcat
     1381 aggctaattc atcccctttc accgagtttt taaaccagct atattacgcc caggtgtcaa
     1441 tgtatgcgcc ttttttactg atgtcatcgg caaaccaggt ggcatacagc cctgcgctat
     1501 agcctctgac actccctttc gaacgataat ccgacacgct ggaatgagtt gagttgtagt
     1561 cgcgggcata accagccaac actcccaaac gccaactgtc actgtcggta aaaccaccgg
     1621 ttaacagatc cgcccctaac tgcgagacgt agcgatgcga ggtcgttctc aactgtccgt
     1681 tgctgtcacg ccaggcatta tgcccgccaa tttgacgtag ccaaagccgg cttgaacgct
     1741 cctgttaagt tacaggatcg atgtaacgcg tttcacctgc acgatcgttc agacgcataa
     1801 caaaaagagt gttagcggct gccaggttgg cgacgtaact tccagcttca gggcgcagga
     1861 cgggggtagg cgcaacagta ggcgctgaac ttcctccgga gtttgtttca ccgccgctcg
     1921 ttgcctggaa gttagtcaga taccagtttt tattggtcgc ttcggtatta cctcgggtta
     1981 atgaatattc gtaggcaccg gcgaaaatcc tcgaatcctt aataaattct ccatttgatt
     2041 ccccctcaac gctgataatc tctatcccat ttaacgtgta agccccgctg ccgccagcat
     2101 tggtaacttt aactcgggtt gttccagagg tatttccatt aataacgact ttatccgtag
     2161 ctgaattatc atcacccaat tcagttctga gatgcaatac gccattatta ccaacgtaat
     2221 tttcagttat tgtcaatctt gttggttcga aatcctttcc atctgcccgg gaaatataga
     2281 ccgtactatt atcaaccgtg agtttgctca ccgttgagtc agctttaata tcccaggtac
     2341 tattatctga aagcgacaga taggtatgag tgttatcatc tgttgaaaga ttggctgaac
     2401 ccgttatttt tgaattagtt gcatcgacgg tcaaatgacc tttaatatta ttaataaaaa
     2461 catctgcagc cgatgatacc tcactatccg taatggtcac gttactcgtc tcatttttat
     2521 ggtagaggat ttcatttgct gaccgtagtg agctaccaga taaaatatct accgttgctt
     2581 cctgaccatt tatggaagac tccagcacaa ttccagcccc ctgatgcgcg actgcagatg
     2641 tatttttcag cgtcaccttg ccgccattat aaattttagc aatcgcggcg tcaggacccg
     2701 ttgtataaat ttctccctgg ttaaatgaaa ctgtcccctg gcgagcagcg atggcatgag
     2761 cattgtcccc atgcgtggtg atatcaacat cggttaatgt aacgctggca ttggtataat
     2821 caagcttgct accctccttg acgacagtgc cattagcgta agcggcataa ctttctgttc
     2881 cgtgggttgt tatctcccct tttgccatat ccagttttgc tttggcgtta ttttcattac
     2941 ttcctgtatc ggcagtactt tcttcatcaa gagaagatac acacgcacgc gcgcttccgg
     3001 gagatgcaca cgcaagaacg ccgactgcgt gattacccgc agtaacgata aaagtattac
     3061 ctccgtcaga aaaagagggg gtagccgaac cgtcagggtt gactcttatt atggtatcag
     3121 tattcaggtt tccatcaact tgacttaaca aaccaacaga ataatctccc gtcgtgacaa
     3181 tcgtattatt accccgcaaa gtgacagcac cgccatattc acttacgact gcgctagctg
     3241 tatatgctgt gcttagagaa ctattgtcag aaggatataa accgcccgtt gttacaatga
     3301 cgccattgtt aatggttaaa ttgctaccct ttcctgcaac agatacaccg taacttccat
     3361 tacccgttgt tataactagc gcattatcaa tatcttttac ttctccatct tttatttcaa
     3421 caacatgttt aggttcatcg tgctgaataa actgttcagc atatgcatta cccaccgcca
     3481 aagcaataag tcctaaacta aacccgggga gttttttcag tttcatagat taaccatcca
     3541 ttcattggat taacaaacat tcctggtcaa ataacctatg agtt
//
LOCUS       DAZKGF010000296         3559 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZKGF010000296 DAZKGF010000000
VERSION     DAZKGF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.296"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            57..614
                     /gene="dsbE"
                     /locus_tag="ACHOQV_004948"
     CDS             57..614
                     /gene="dsbE"
                     /locus_tag="ACHOQV_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFP9633180.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKL
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            611..1663
                     /gene="ccmH"
                     /locus_tag="ACHOQV_004949"
     CDS             611..1663
                     /gene="ccmH"
                     /locus_tag="ACHOQV_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFP9633181.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKAAPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(1698..2345)
                     /gene="narP"
                     /locus_tag="ACHOQV_004950"
     CDS             complement(1698..2345)
                     /gene="narP"
                     /locus_tag="ACHOQV_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFP9633182.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            2665..3424
                     /locus_tag="ACHOQV_004951"
                     /pseudo
     CDS             2665..3424
                     /locus_tag="ACHOQV_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          889 a          841 c          959 g          870 t
ORIGIN      
        1 tttgatcctc gctatcgtaa gcgcgtgaac ccgcagaaaa ctgcgccgga ggctgtatga
       61 agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctactgt
      121 ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg
      181 gcaagctagt gccgaagttt cgcctcgaat cactggacaa tccggggcag ttttatcagg
      241 cggatgtgct gactcagggc aaaccggtgc tgcttaacgt ctgggcgacc tggtgtccga
      301 cctgccgtgc ggaacatcaa tatctgaatc agctttctgc gcagggcatc cgcgtggtcg
      361 gcatgaacta taaagacgat cgccagaagg caatcagctg gctgaaagag ttgggcaatc
      421 cttacgcact aagcctgttt gatggcgacg gcatgttagg gctggatctc ggtgtctatg
      481 gcgcgccaga aacgttcctt attgacggca acggcatcat tcgctatcgc catgcgggcg
      541 atctcaatcc tcgcgtctgg gaagaagaga tcaagccact gtgggagaaa tacagtaagg
      601 aggccgcaca atgaggtttt tattgggcgt gctgatgctg atgatctccg gctcagcgct
      661 ggcgaccatc gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct
      721 cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc attgccgatt ccaactcgat
      781 gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggca aaagtaagaa
      841 agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt
      901 aacgccgctg accgtgctgc tgtgggtgct gccagtagtg gctattggca ttggcggttg
      961 ggtcatatac gcccgttcgc ggcgtcgggt acgcgtggtg ccggaagcgt ttccagaaca
     1021 aagcgtgccg gaaggtaagc gtgccggata tgttgtttat ctgccgggta ttgtggtggc
     1081 gttaattgtg gctggcgtca gctactacca gactggcaat tatcagcagg tgaaaatctg
     1141 gcagcaggcc accgcgcagg ctccggcgtt actggacagg gcgctggatc cgaaagccgc
     1201 tccgctcaac gaagaagaga tgtcgcgcct tgcgctgggg atgcgtactc aactgcaaaa
     1261 aaatccggga gatatagaag gctggattat gttgggccgc gttggcatgg cgctgggtaa
     1321 cgccagtatc gccaccgatg catacgctac tgcatatcgc ctcgatccga agaacagtga
     1381 tgcggcgctc ggatacgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct
     1441 cggcggcgaa ctgctgcgcc agttggtgag aacggaccat agcaatatcc gtgtgttaag
     1501 catgtatgca tttaatgcct ttgagcagca acgatttggc gaagccgttg ccgcgtggga
     1561 aatgatgttg aaactcttac ctgccaacga tactcgccgt gcggtgatcg aacgtagtat
     1621 cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa taaaaagtgg cccgatggtg
     1681 atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggccgcc
     1741 acgcgtgagc ggacattgag tttacgcagc agattgcgaa tgtgtacttt tactgtctgc
     1801 tcggaaatat tcaacaccga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc
     1861 agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt
     1921 tcacgttcac gtaagtactg attgacgcgt tcgctaaaaa ctttgctgcc tttcgctccg
     1981 gcacgaatcg cttcaagcaa cacttccggg tcgctgtctt tcaacagata accgtctgcg
     2041 cctgcgtcta tcagcgcaaa gacatcgctg gaggcatcgg atacggtcag gataataatt
     2101 tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc
     2161 atattgagat ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc
     2221 gcgccgtcgc ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga
     2281 acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca
     2341 ggcatagtag tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac
     2401 gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac
     2461 tttgtcgttc gctttggcat catcttcatt ataaagacgg aattttgcat cagcttctca
     2521 tcaggaaaaa caactaaaaa atcaatcaca atacttgtgc atttctttaa acagtttttt
     2581 aaaactgact ccgggtatgg agctatgggt attttctgtc cccaatgctt ttaacagcaa
     2641 ttaatttcat aggatgaaag cttaatgcac caatctggtt ctgtttctct ttgtcgttcc
     2701 gcaatatctg ttctggtggc tacagcgtta tattcaccca tagcattggc atcaactgtt
     2761 gagtatggtg agacagttga tggtgttgtc ctggaaaaag atatccagct ggtttatggg
     2821 gccgccaata atacgaaaat caatcctggc ggagaacagc atatataaga atttggtgta
     2881 agtagtaata ctaaaattaa cgggggatat cagtacattg aaataaatgg caccgcagaa
     2941 tactcagtat taaatgatgg ttatcaaatt gttcaaatgg gtggcgcggt aaaccagact
     3001 acgatcaata atggagtgct acaggtttat ggcgcggcga ctgatcccac gattaaagga
     3061 gggcgcggtg atgcggcatt tacgttgggc aatgccggaa gcgttgttga tatcagtacg
     3121 tatgaatata ccttgctgga taatggcaac catagctgga gtctcacaga gaatcgcgta
     3181 caaatgcccc cttcaaccac ggatgtgctg aatatggcgg cagcacaacc gctggtattt
     3241 gatgcagaac tggacaccgt gcgtgagcgt cttggtagcg taaagggcgg cgatgtggag
     3301 ttcggcaatt aacagccgca acaacgtgaa cactgatgcg ggagctggct ttgagcaaac
     3361 attgacgggc ctgacgctcg gtatcgatag tcgtttctcc cgtgaagaaa gcagcacaac
     3421 tcaggcgggt gtagtctgga cgttctgata acagaaaata aacaggtcgt gatgtgtcgc
     3481 tgtctgttta tcgaattaaa tgcagatata aaaaaaccaa ccgcaatagg ttggttttct
     3541 tgggattttt ggtccacga
//
LOCUS       DAZKGF010000297         3553 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZKGF010000297 DAZKGF010000000
VERSION     DAZKGF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.297"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(46..1905)
                     /gene="gss"
                     /locus_tag="ACHOQV_004952"
     CDS             complement(46..1905)
                     /gene="gss"
                     /locus_tag="ACHOQV_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFP9633183.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPIAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFSVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2110..2976
                     /gene="yghU"
                     /locus_tag="ACHOQV_004953"
     CDS             2110..2976
                     /gene="yghU"
                     /locus_tag="ACHOQV_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFP9633184.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3099..3347)
                     /gene="hybG"
                     /locus_tag="ACHOQV_004954"
     CDS             complement(3099..3347)
                     /gene="hybG"
                     /locus_tag="ACHOQV_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFP9633185.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3360..>3553)
                     /locus_tag="ACHOQV_004955"
     CDS             complement(3360..>3553)
                     /locus_tag="ACHOQV_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase/urease nickel incorporation protein"
                     /protein_id="HFP9633186.1"
                     /translation="VCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHG
                     ERLRVDTGDSLIVKSIEVE"
BASE COUNT          836 a          967 c          898 g          852 t
ORIGIN      
        1 tactgattat tcttcataat cagtacgggg ttgctacaac atgtattact ctttcaccac
       61 aattagcggt tcaatatcac tctctttttt gatgaccagt gattcatcac cgcgcaaaca
      121 cgtcccaccg tagttgccgc caacggtgaa ggtacatacc tgaatgtatt taccgtccac
      181 tttcggcaaa caccacagtt gctgatagat gtttttctgc tcggcaaatt taccgctggt
      241 tttgtccagc acctcttcat gatggctgac gaggtcgatg ttactgccac agcgaccggc
      301 gatcggtttc actgcataac cggttttcac cagttcatca ttcacggaga aatcggtatc
      361 cagcaggtaa cgatggtgcg ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc
      421 ggggatcact gtccacagag gttcaaagac cagcacttcc gggcgcagca atacgtcgat
      481 aagacgcact tcattttgcg gatgaccggt acggatcggc accgcagcaa actcacggtc
      541 gctaacttca cgaatctgat caaacgcggt ttcccacgcc caggttttcc acacgcagtt
      601 aaccagtcgc ccttccccat caatcagttg cccggcagca tcccagccca gttcatccag
      661 cccgcgcaag atacgcgttt caaagcccgc ctggtgcagc gcctgctcca taaactgcgc
      721 gtgatagttt tcctcgatat ctttgtcctg catgatatgg acaaacggac gtgcacgact
      781 gtgtttccag gcaccagcca gttcgttaat cagcccttcc gccggattga agccgttgcc
      841 tttatagccc tgctccgccc aacgttcgag gatcaagcca gcttcggtat gacaggaggc
      901 ggagtcggcg ttgtattcgt agaccttcag gccacgttcg tccatacaga aatccatacg
      961 accggtgatc atatggtgac ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat
     1021 ttttgggatg tcgaacagcg ccagcaggtt gtcatctttc agcaccttgt cggttgcgtg
     1081 aagatacatc aggtgcagtt cgttggtggc tttaatcagc tcctgctctg cgctctcggt
     1141 aatggtgtag taatgatacg gatcctgatt gatcacctga ccgttggcct gcacataggc
     1201 gttttgtagc ggatcttttt catccagcca tttaccgtca aactggcctt tgttttccag
     1261 gcgcgctccg ctgattttca acagctcgcc tgcaatttcc ggctgcggta agctgtattc
     1321 agtatcttcc gtctggatca tccagccgag aatggtggtg tcatcaaaag tgtctttaag
     1381 ggtatagcag ccgttttcga ccaccatctc cagctcgcgc gtccactgtt gcccttgcgg
     1441 taacggggaa tgaatcacgt tctgttccgc aatacggact ttgttgccat gcaattgggt
     1501 aatgatggcg acatgaccag tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc
     1561 acccgcgatc ggcgcacgcg gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc
     1621 attaaccact tcacgcagga agcgcagcga gaaaatctcc cacgccatac ccacgtcagt
     1681 gaagaccaca ccgtaattca gaaagagaaa acggcgagca aattcaacgc attgccattt
     1741 gtggcccata tattcgtcgt cgatatagct acggaatacg gcgtcatctt cgtattcctg
     1801 cggatcgaga gaactgtaat ctgaagagta gattgctacc ccacctgggg cgtagcccaa
     1861 taatgtcccg aacggggcat cctggctggt cgttcctttg ctcatcactt tacctttaac
     1921 aatacaacct aagcagttgg cgtaattgtt gtgctctgat tacctgctca gcgacattaa
     1981 ccggacagag gtcagactaa tcatacacct catcgcagcg gctggcgcaa agggttaaaa
     2041 aattcacatt ctgtacagca agtgacctgc tacactgctt caacactacc actcagaagg
     2101 caactcacta tgacagacaa cacttatcag cccgcgaaag tctggacgtg ggacaaatcc
     2161 gccggcggcg cgttcgccaa tatcaatcgc ccggtttctg gtccgacgca tgaaaaaacg
     2221 ctgcccgttg gcaaacaccc attgcaactt tattcgctgg gaacgccgaa cggtcagaaa
     2281 gtaacgatta tgcttgagga gctgctggcg ctgggcgtta ctggtgcaga gtacgacgcc
     2341 tggctgattc gtattggcga tggcgatcaa ttctccagcg gctttgtcga agtgaaccca
     2401 aactcgaaga tcccggcgct gcgcgatcat acgcataatc cgccgatccg cgtgtttgag
     2461 tctggatcga ttctgcttta tctggcggag aaatttggct acttcctgcc gcaggatctg
     2521 gcaaagcgta ctgaaacgat gaactggctg ttctggctac agggggctgc accgttcctc
     2581 ggcggtggtt ttggtcactt ttaccattac gcgccggtga aaattgagta cgccatcaat
     2641 cgctttacca tggaagccaa gcgcctgctc gacgtgctgg ataagcaact ggcgcagcat
     2701 aagtttgttg caggcgatga gtacaccatt gcggatatgg cgatttggcc gtggtttggc
     2761 aacgtggtgt taggcggcgt gtatgatgcc gcagagtttc tggatgcggg aagttataaa
     2821 cacgtacaac gctgggcgaa agaagtgggc gaacgtccgg cagtgaagcg tgggcgtatt
     2881 gttaaccgca tcaacggacc gctgaacgag cagttgcatg aacgccatga cgccagtgat
     2941 ttcgagacga atacggaaga taagcgtcag gggtaagggt tggtattcgt cgcagcaagc
     3001 catccaggcc ggataaggcg ttcacgccgc atccggcaat cgagcacaat gcctgatgcg
     3061 acgctgccgc gtcttatcag gcctacaaaa gctaatcatc acgcgctggt aatgtcgtaa
     3121 tccatttggc gcagcgcgtc taatgtggct ttggcttcat cttcgtcgat gatgctcatg
     3181 gcaaatccga cgtgtaccag tacccactgg cccagcagat cggcagggtt accttcacaa
     3241 atcagggcga tattcacgtc gcgcttgata ccacatactt caacctgcgc aagctggtga
     3301 atatcttcac cgacagccag cacctggcct ggaacgccaa tacacattgt taactccggt
     3361 tattcaactt caatactttt gacgattagc gaatcgccgg tatcgacacg caaccgctcg
     3421 ccatgacaga tcggacactg cgcatcgtgc tgatgaatct ccaccacctg gctgcaatcc
     3481 cagcaccagg cctgggcggg tttatagacg atatgtaaat cgcacccttg cgccaccgtt
     3541 ccgtggcaga caa
//
LOCUS       DAZKGF010000298         3510 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZKGF010000298 DAZKGF010000000
VERSION     DAZKGF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.298"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(94..205)
                     /locus_tag="ACHOQV_004956"
                     /pseudo
     CDS             complement(94..205)
                     /locus_tag="ACHOQV_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(645..1487)
                     /locus_tag="ACHOQV_004957"
     CDS             complement(645..1487)
                     /locus_tag="ACHOQV_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9633187.1"
                     /translation="MSDITVSRPEVVNWHTDVICSTSILHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFHCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLEIMLHLFSGKPIPDNR
                     ENITIHLDDHVQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     ALAI"
     gene            complement(1572..1769)
                     /locus_tag="ACHOQV_004958"
     CDS             complement(1572..1769)
                     /locus_tag="ACHOQV_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633188.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGILFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            complement(1781..2020)
                     /locus_tag="ACHOQV_004959"
                     /pseudo
     CDS             complement(1781..2020)
                     /locus_tag="ACHOQV_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            2087..3249
                     /locus_tag="ACHOQV_004960"
     CDS             join(2087..2348,2348..3249)
                     /locus_tag="ACHOQV_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFP9633189.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            complement(3246..>3510)
                     /locus_tag="ACHOQV_004961"
     CDS             complement(3246..>3510)
                     /locus_tag="ACHOQV_004961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633190.1"
                     /translation="NINEQEVFKMREISDNMLDSVKGGMNLNGLPGSTNVIDLRGKDM
                     GTYIDANGACWAPDTPSIIIYPGGSGPSYSMSSDPTHVIWTQA"
BASE COUNT          868 a          841 c          870 g          931 t
ORIGIN      
        1 ccagaagtaa tattccttac catcaaaatt catcatcttt gccaaagaaa aatgttcaga
       61 aaataatcca tggaaaattg tccggagcgc ttactatttt aatggattgt tagtctttgc
      121 atgagcaaac ggactgatac atttctcttt gttctcattc agaaaatctc atcagttggc
      181 gttctgcccg atgttgtgct ttatgggtgc gttacgctcc tgatgacgtc atttgacgtt
      241 caacagcatc acggggccgc acgacatttc acgtcagtca gcgctatagc tcagaaacaa
      301 attttcccga attggaatat gcccgcaaat tgctggggat gtggggagaa tctggttgag
      361 ttcggtagaa ttgatttggc ggcaggacgc ttaaatcgtg gcgtcctgaa acgaaaaacg
      421 gacctccgtg gaggtccgtt tatatgaatt tggtgccgga ctcggaatcg aagatttcat
      481 ttatcatatt gatattaatc tcgtttattt ggtttgatga aaaatgtacc ctctcttgta
      541 ccctcggtcc cttctggtgg tttttatgag aacaagtaga tcaagatttt tgtcatcaga
      601 acacgtgtat ttttgtgggt tttattcctg agttttattc ctttttatat cgctaacgct
      661 ccgggatagt gcttagcaat aatatcatta agcctgtcaa ccagctctgg tttcctgaac
      721 gtgatgtgcg cggagccctt cttaaagtat ctgatgctga acatctcatc ttcatagtcc
      781 tctttaccct gaacagactg gacgtgatca tccagatgaa tagtgatgtt ttcccggttg
      841 tcggggatcg gtttgccgct gaacaggtgt agcattattt ccagatcagc aagtcgatct
      901 gcctgttgcc cggtgataag atgaaagccc caccggtccc acctcaccag gttgttgacg
      961 ataattttac tgccaaattt gcaggggcaa ttggttttgt aattccagct caaccctctg
     1021 aagacgttga tcaccccccg ctcaaacacc tcatccttat tctggtgcag ttgttcaaat
     1081 gtgctcagaa tgttcgcttc actgatttcc ggaaaattat cgtattccag tgaccggtac
     1141 catgtatcac gggcctgtgc atccattaag gaaagcatcc cggaacgttg catcaggtca
     1201 cgccagattt cgcgatcgag attgcgtgtg atggctttca ttgcggtttc aggtttctcc
     1261 atcagccagc aaccgcagtg aaaattctgt ttcatggccc agtccagtgc ggttttaccg
     1321 ccaatgcttt ctgtcattgc tgagatttca gccagttgcc ggataagtgt gtcgatttgc
     1381 agcagtgtac tctttcgtac agccagaatg tggaggatac tggttgagca gataacgtcc
     1441 gtatgccagt tgacaacttc cggacgggag actgtgatat cagacataat cgatattcct
     1501 ttttgcagat aaatgaaaac gccagccctg ttcaggctgg cgtcagtgga atgaagtctg
     1561 gtgagtgaga gtcaccggtt ctggtgcaga agctgcaact ggcgcagtgt acggatatct
     1621 tccctggcct gttcaatgca gggcagcagt gctgccgaat ccgttttatc ctcatcgttg
     1681 tcaaagagaa ttcccgattc gcagtcgata ttgtcctgca gccacgcaat cagaatatcc
     1741 agcgctgttt ccgtggttaa tgatttcatg ttgtttctgg ttaccagtcg aaagtgggta
     1801 aacccggcag gcattcagcg ctggcatcca gatgaatgag actgacgcca taacgtcggg
     1861 taagtgtgac aaccagacgg cggaacgttt ttgacagccc caggcgttta agccgcagaa
     1921 ccgggtatga ccacgcatcg gtacgtaaca gatatccgct acctgtgaaa tgaatccatt
     1981 ctgattcgcc ggtgtcattc gtctgatgtg acagcgagta ctgatcttac ccagcaatag
     2041 tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacatga caaaaacagt
     2101 atcaaccagt aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg aagccctgaa
     2161 gcttgctgaa cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt atgaatcaca
     2221 gctctacaac tggcgcagta aacagcaaaa tcagcagacg tcttctgaac gtgaactgga
     2281 gatgtctacc gagattgcac gtctcaaacg ccagctggca gaacgggatg aagagctggc
     2341 tatcctccaa aaggccgcga catacttcgc gaagcgcctg aaatgaagta tgtctttatt
     2401 gaaaaacatc aggctgagtt cagcatcaaa gcaatgtgcc gcgtgctccg ggtggcccgc
     2461 agcggctggt atacgtggtg tcagcggcgg acaaggataa gcacgcgtca gcagttccgc
     2521 caacactgcg acagcgttgt cctcgcggct tttacccggt caaaacagcg ttacggtgcc
     2581 ccacgcctga cggatgaact gcgtgctcag ggttacccct ttaacgtaaa aaccgtggcg
     2641 gcaagcctgc gccgtcaggg actgagggca aaggcctccc ggaagttcag cccggtcagc
     2701 taccgcgcac acggcctgcc tgtgtcagaa aatctgttgg agcaggattt ttacgccagt
     2761 ggcccgaacc agaagtgggc aggagacatc acgtacttac gtacagatga aggctggctg
     2821 tatctggcag tggtcattga cctgtggtca cgtgccgtta ttggctggtc aatgtcgcca
     2881 cgcatgacgg cgcaactggc ctgcgatgcc ctgcagatgg cgctgtggcg gcgtaagagg
     2941 ccccggaacg ttatcgttca cacggaccgt ggaggccagt actgttcagc agattatcag
     3001 gcgcaactga agcggcataa tctgcgtgga agtatgagcg caaaaggttg ctgctacgat
     3061 aatgcctgcg tggaaagctt ctttcattcg ctgaaagtgg aatgtatcca tggagaacac
     3121 tttatcagcc gggaaataat gcgggcaacg gtgtttaatt atatcgaatg tgattacaat
     3181 cggtggcggc ggcacagttg gtgtggcggc ctcagtccgg aacaatttga aaacaagaac
     3241 ctcgcttagg cctgtgtcca tattacgtgg gtaggatcac tactcatact ataagagggt
     3301 ccacttcccc ccggatatat gatgatggat ggagtatccg gagcccagca tgctccatta
     3361 gcatcaatat atgttcccat atcttttcca cgtagatcta ttacattagt agaaccaggt
     3421 aatccattaa gattcatccc tcctttcacg gaatcaagca tgttatctga tatttctctc
     3481 attttaaaaa cctcctgctc atttatgttc
//
LOCUS       DAZKGF010000299         3490 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZKGF010000299 DAZKGF010000000
VERSION     DAZKGF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.299"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1031)
                     /locus_tag="ACHOQV_004962"
     CDS             complement(<1..1031)
                     /locus_tag="ACHOQV_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-binding domain-containing protein"
                     /protein_id="HFP9633191.1"
                     /translation="MTDITANVVVSMPSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVAQPIVINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQFY
                     FPNVLKYDPDQLEQRLSSTSGASLIGIDKYGTIENAIKYSIKYVTPEMYISQSSDITE
                     AIQMAIDDCEIVYMEPTDYLIERTINIPDGRVIYLGGSKLTASTGSSPIFKYNGKNIG
                     LTIWGGSCIVTGTASAFLEAEGDSDTPSNEDYIKQIRLYGVHVSSSMITYALLLKKAV
                     RQIFIDSCMFYTVNGIVSSGKTVELACNKSIVFGATSATDTVGIGIYSTATGGPYYNE
                     GWHFTDCTI"
     gene            complement(1132..2010)
                     /locus_tag="ACHOQV_004963"
                     /pseudo
     CDS             complement(1132..2010)
                     /locus_tag="ACHOQV_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
     gene            complement(2079..2240)
                     /locus_tag="ACHOQV_004964"
     CDS             complement(2079..2240)
                     /locus_tag="ACHOQV_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9633192.1"
                     /translation="MKGMSKMPQFNLRWPKEVLDLVRKVAEENGRSVNSEIYQRVMES
                     FKKEGRIGA"
     gene            2331..2585
                     /locus_tag="ACHOQV_004965"
     CDS             2331..2585
                     /locus_tag="ACHOQV_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9633193.1"
                     /translation="MARDDPHFNFRMPLEVREKLKYRAESNGRSMNSELLQIVQDALS
                     RPSPVKGYRNEAERLAEMQAEQFKSVVFETLKNMYGKDAK"
     gene            2582..3163
                     /locus_tag="ACHOQV_004966"
     CDS             2582..3163
                     /locus_tag="ACHOQV_004966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633194.1"
                     /translation="MIDYNPAKKEIIAAKKCLEQMKSSTNHDDFEMHWRECLGHIEKS
                     FSKLLCATKPVSGKFSSHFNQKFMLRKTDKTLAYLHQARNADHHSTMEISKLEPPYTT
                     LGAFPGARSHYIKELIIDSTGKIAKYEGDPMIVEFHPATTMPITVRNQGKDYPPPTEH
                     LGEKLVDIHPSVLAYLGIQFYEKWIDESRAMFR"
     gene            3309..>3490
                     /locus_tag="ACHOQV_004967"
     CDS             3309..>3490
                     /locus_tag="ACHOQV_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein A"
                     /protein_id="HFP9633195.1"
                     /translation="MRNLVLSIRISYRDKVTRKTKEVGVSDSMSYAVLVAATLFMGIG
                     LQIAWFFFSSFIKRKR"
BASE COUNT         1015 a          751 c          664 g         1060 t
ORIGIN      
        1 tctattgtgc aatcggtaaa atgccaaccc tcattataat aagggccacc ggttgcagta
       61 ctataaatgc caattccaac agtatcagta gccgatgtcg ccccaaatac aatgctctta
      121 ttacaagcca actctactgt tttccctgat gaaacaatcc catttacggt ataaaacatg
      181 caggaatcaa taaatatttg acgaactgct ttttttaaca aaagcgcata tgtaatcatt
      241 gaagatgata catgaacacc gtaaagtctt atctgtttaa tatagtcttc attgctaggt
      301 gtgtcgctat caccttcagc ctctaagaat gcacttgccg ttcctgtaac aatgcagctg
      361 ccgccccaaa ttgttaatcc gatattttta ccattatact taaatatagg agaactacct
      421 gtagaagctg ttaactttga tccgccaaga taaataaccc tgccatcagg tatatttatt
      481 gttctttcaa taaggtagtc ggtaggttcc atatatacaa tttcacaatc atcaattgcc
      541 atctgaatag cctcggttat gtctgaggac tgtgaaatat acatttctgg tgttacatac
      601 tttattgaat atttaattgc attctcaatt gtaccatatt tatctatacc tattaatgat
      661 gctcctgatg tgcttgaaag tctttgctcc agctgatcag ggtcatactt cagcacatta
      721 ggaaaataga actgctgcgc accatatgca tcataaacag ccatagaatg gccttgcacg
      781 gtaacgaatt tggcaatctg tccgttatat actggatacc cagcggcatt gatgacgatt
      841 ggctgtgcaa caggaacgtg agaaccatct tcgttctcta cataaacctg aatttggttt
      901 tcaggattta ccgggtcagt gtcaatttta ccgatataaa tttttccatt ggctacggct
      961 ttaaaagaac gcgccatagt gaagagttgc gaaggcatgc tcactacaac attggctgta
     1021 atgtctgtca tttaatttgc tccagataca aggaatcgcc gcagcatggc tacggtgaat
     1081 tttgggcata aaaaaaccca gccgaagctg ggtcgttgag ttggttatct gtcagtagtt
     1141 atgtactgaa ggaggtaatt ctttattctt aagtctcatc catgcggaaa gattcgttgg
     1201 tccgtctggc tcattaatat caacatctcg tgtatggttg attaaaacat ctctcgccat
     1261 tccgataaca tacgagaact catgaccgta gtcgtagcat ctgccggaat agttcgattg
     1321 aatttgtttt aatgccggat acagttcacg gaataatgcc tgtgaacggt tggcataatc
     1381 ccatagccat acaaggctgt ttgcttcttt tgcagaaagc tcgtttgctt tcttctcttg
     1441 tttgccgata aactcgcctt caagcactac cctgtggatg tactatacgg ccagcgggat
     1501 ttgttcaatt gaaagttcat caatgctgtc aataccaaaa cgctgatgaa ccatattgta
     1561 tgcatcgtca tagcgaagtc ctttctttcc taccagcatg tttactgcat cgcgtagcgg
     1621 cgttctttcc tcaacagtgg ttttcttgcc tttcacatac tcgccatgtt tgcgaattga
     1681 aggtagaact tctgctgtta cccacttgcg gaatttgtgc gggaccgaac ctttattgac
     1741 agcatcgcgg cagcgcagaa ccaatgtata catacctgat tcgctaacaa tgcttagatt
     1801 ctgctcacca ccaagggtgt aacttaaagt tactcccttt tcatcgtcat caagtgcagt
     1861 aatcgccttg cgtgagttag tcagggttaa agcatcacaa acatcttttg ctacaaacca
     1921 cggctcaccg catttgttga tgacgcggat ttcactgtcg ccgaatttga agatggtgaa
     1981 atcgttttgt gcctttgcta tacttttcat gtcaatattt cctaatccga tttgttgata
     2041 ccgaagccct gactgttccc gcagttgggg cttcaacttt acgcgccaat gcgcccttcc
     2101 ttcttaaagc tttccattac tctctgataa atctcagagt taacagaccg accattctct
     2161 tccgccacct tgcgtaccaa atccaatact tctttaggcc accgcaaatt gaactgcggc
     2221 atcttgctca ttcctttcat attcacctca caatataggt ccaccgtgga cctattgaga
     2281 atatagtaga gtgcttctat catgtcaata cactaacttg gagtgatggc atggctagag
     2341 atgatccgca ctttaacttc cgcatgcctt tggaagtaag agaaaaatta aaatacagag
     2401 cagagtcaaa tggtagatca atgaactcag aattgttgca aatcgtccaa gatgctctat
     2461 cgagaccatc tcctgtaaaa ggttatcgca atgaagctga acgattagcc gagatgcagg
     2521 ctgaacagtt caagtctgtg gtatttgaga cacttaaaaa catgtatggc aaggatgcaa
     2581 aatgatcgat tacaacccag caaaaaaaga aatcatcgca gctaaaaagt gcctagagca
     2641 gatgaaatca tctacaaacc atgatgattt tgaaatgcac tggcgagaat gtcttgggca
     2701 tatagaaaag tctttcagta agttgctctg tgcaacaaag ccggttagtg gaaaatttag
     2761 cagccacttc aaccaaaagt tcatgcttag aaaaacagac aaaacacttg cttaccttca
     2821 ccaagcaaga aatgctgatc atcattccac tatggaaatc tcaaaacttg aacctcctta
     2881 tactactctt ggggcatttc cgggagcaag aagccattat attaaagagt taatcattga
     2941 ttcaacaggg aaaatagcaa aatacgaagg cgaccctatg atagttgagt ttcatccagc
     3001 aacaactatg cctataaccg taagaaacca aggcaaagat tatccacctc caacagaaca
     3061 tcttggcgaa aagcttgttg atatacatcc atctgttctt gcctatctcg gtatacaatt
     3121 ttatgaaaaa tggatagatg agtcgcgtgc aatgtttaga tagttatttt tgataatttc
     3181 aagtatttcc tcttgacttt gatttaccca aacgtgatca cactcatgaa accagattat
     3241 gcttcctgtt tcgccttgtg caatataggt gatcatgttt tcattaacaa ttattactga
     3301 gttatcatgt gcgcaattta gttttatcaa ttcgcatatc ctatcgtgac aaagtaacta
     3361 gaaaaactaa agaggttggt gtgtctgatt ctatgagtta cgctgtgcta gttgccgcaa
     3421 ctctatttat ggggataggg ttgcagattg cgtggttctt tttttctagt tttattaaac
     3481 gcaaaagaat
//
LOCUS       DAZKGF010000300         3486 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZKGF010000300 DAZKGF010000000
VERSION     DAZKGF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.300"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..186)
                     /locus_tag="ACHOQV_004968"
     CDS             complement(<1..186)
                     /locus_tag="ACHOQV_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HFP9633196.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIV"
     gene            501..1817
                     /gene="shiA"
                     /locus_tag="ACHOQV_004969"
     CDS             501..1817
                     /gene="shiA"
                     /locus_tag="ACHOQV_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFP9633197.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            1919..3373
                     /gene="amn"
                     /locus_tag="ACHOQV_004970"
     CDS             1919..3373
                     /gene="amn"
                     /locus_tag="ACHOQV_004970"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFP9633198.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          827 a          844 c          832 g          983 t
ORIGIN      
        1 tacaattaaa agatgcttaa ttttatgcaa ttcaagcgcc cgaataacag gaaagatggc
       61 catagcatcg ccaatctgat cgggaatatg gatgacaaca aagtctgttt tttcaatatt
      121 gaaattataa gctttataat cgtagtaact aaatgcaata cgtctcaaca atgatgctaa
      181 aaacatacct aacctcgcct ccctactggt tataatgcaa tgcagtctat cagactcatc
      241 agggtgccat tttgtgcata tgcggacttt tatgtttcat atctcgaacc tgtgaggcct
      301 ctgcttaatc cttaaacaac accagcaact cctgcgcttt catcttccag cgaatttttc
      361 atgttgctgc tcatcagcca taaaatcatt tgcagatgcg ctctgtcgag gtagtctcat
      421 aaggttcgtt tatagatcga cggcaatgtg agttaccttt tccatactaa ttataaaaag
      481 acagtacaaa caggatcatt atggactcca cgctcatctc cactcgtccc gatgaaggga
      541 cgctttcgtt aagtcgcgcc cgacgagctg cgttaggcag tttcgctggt gccgtcgtcg
      601 actggtatga ttttttactc tatggcatca ccgccgcact ggtgtttaat cgcgagtttt
      661 tcccgcaagt aagcccggct atgggaacgc tcgccgcatt tgccaccttt ggcgtcggat
      721 tcctgttccg tccgctcggc ggtgtcattt tcggtcactt tggcgaccga ctgggacgta
      781 agcgcatgtt aatgctgacc gtctggatga tgggcatcgc gacagccttg attggtattc
      841 ttccttcatt ctcgaccatt gggtggtggg cacctatttt gctggtgaca ctgcgtgcca
      901 ttcagggatt tgccgtcggc ggcgaatggg gaggcgcggc gttgctttcc gttgaaagtg
      961 cgccgaaaaa taaaaaagcc ttttacagta gcggtgtaca agttggctac ggtgtaggtt
     1021 tactgctttc aaccggactg gtttcattga tcagtatgat gacaactgac gaacagtttt
     1081 taagctgggg ctggcgcatt cctttcctgt ttagcatcgt actggtactg ggagcattgt
     1141 gggtgcgcaa tggcatggag gagtccgcgg aatttgaaca acagcaacat aatcaagcgg
     1201 ccgcgaaaaa acgcatcccg gttatcgaag ctctgttacg acatcccggt gctttcctga
     1261 agattattgc actacgactg tgcgagttgc tgacgatgta cattgttact gcctttgcac
     1321 ttaattattc aacccagaat atggggttac cgcgcgaact tttccttaat attggtttgc
     1381 tggtaggtgg attaagctgc ctgacaattc cctgttttgc ctggcttgcc gatcgttttg
     1441 ggcggcgcag ggtttatatc acaggtgcgt taatcggaac gttgagcgca tttcctttct
     1501 ttatggcgct tgaagcacaa tctattttct ggatagtttt cttctccatc atgctggcaa
     1561 acattgcgca tgatatggtg gtgtgtgtac aacaaccgat gtttaccgaa atgtttggtg
     1621 ccagttatcg ctatagtggc gctggagtcg gttatcaggt tgccagtgtg gttggcggtg
     1681 gatttacacc ttttattgcc gctgcactca tcacttactt tgccgggaac tggcatagcg
     1741 tcgccattta tttgctggct ggatgcctga tttccgcaat gaccgctttg ttgatgaaag
     1801 acaatcaacg cgcttaatag cctggcgaag atcatccgat cttcgcctta cacttttgtt
     1861 tcacattcct gtgacatact atcggatgtg cggtaattgt atggaacagg agacacacat
     1921 gaataataag ggctccggtc tgaccccagc tcaggcactg gataaactcg acgcgctgta
     1981 tgagcaatct gtagtcgctt tacgcaacgc cattggcaaa tatattacaa gtggcgaatt
     2041 acctgatgaa aacgcccgca aacaaggtct ttttgtctat ccatcactga ccgtaacctg
     2101 ggacggtagc acaaccaatc cccccaaaac gcgcgcattt ggtcgtttta cccacgcagg
     2161 cagctacacc accacgatta ctcgccctac tctctttcgt tcgtatctca atgaacaact
     2221 tacgttgctg tatcaggatt atggtgcgca tatctcggtg caaccctcgc agcatgaaat
     2281 cccttatcct tatgtcatcg atggctctga attgacactt gatcgctcaa tgagcgctgg
     2341 gttaactcgc tacttcccga caacagaact ggcgcaaatt ggcgatgaaa ctgcagacgg
     2401 catttatcat ccaacggaat tctccccgct atcgcatttt gatgcgcgcc gcgtcgattt
     2461 ttccctcgcg cggttgcgcc attataccgg tacgccagtt gaacattttc agccgttcgt
     2521 cttgtttacc aactacacac gttatgtgga tgaattcgtt cgttggggat gcagccagat
     2581 cctcgaccct gatagtccct acattgccct ttcttgtgct ggcgggaact ggatcaccgc
     2641 cgaaaccgaa gcgccagaag aagccatttc cgaccttgca tggaaaaaac accagatgcc
     2701 agcatggcat ttaattaccg ccgatggtca gggtattact ttggtgaata ttggcgtggg
     2761 accgtcaaat gctaaaacca tctgcgatca tctggcagtg ctacgcccgg atgtctggtt
     2821 gatgattggt cactgtggcg gattacgtga aagtcaggcc attggcgatt atgtacttgc
     2881 acacgcttat ttacgcgatg accacgttct tgatgcagtt ctcccgcccg atattcctat
     2941 tccgagcatt gcggaagtgc aacgtgcgct ttatgacgct accaagttag tcagcggcag
     3001 gcctggtgaa gaagtcaaac agcggttacg tactggcacg gtggtaacca cagatgacag
     3061 gaactgggag ttacgttact cagcttctgc acttcgtttt aacttaagcc gggccgtagc
     3121 aattgatatg gaaagtgcaa ccattgccgc gcaaggatat cgtttccgcg tgccatacgg
     3181 gacactactg tgtgtttcag ataaaccgtt acatggtgag attaaacttc ccggccaggc
     3241 taaccgtttt tatgaaggcg ctatttccga acacctgcaa attggcattc gggcgatcga
     3301 tttgctgcgc gcagaaggcg accgactgca ttcacgtaaa ttacgaacct ttaatgagcc
     3361 gccgttccga taataagtta tgaaccttct tatcagaaat agatgtgaag gattaagtaa
     3421 gacatctggc aaatagcctg caataaacgc tggctgtttg cttccttggg cagatgcgag
     3481 ttttat
//
LOCUS       DAZKGF010000301         3431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZKGF010000301 DAZKGF010000000
VERSION     DAZKGF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.301"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(41..667)
                     /gene="dsbA"
                     /locus_tag="ACHOQV_004971"
     CDS             complement(41..667)
                     /gene="dsbA"
                     /locus_tag="ACHOQV_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFP9633199.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(684..1670)
                     /gene="srkA"
                     /locus_tag="ACHOQV_004972"
     CDS             complement(684..1670)
                     /gene="srkA"
                     /locus_tag="ACHOQV_004972"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFP9633200.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTPDQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1747..2016)
                     /locus_tag="ACHOQV_004973"
     CDS             complement(1747..2016)
                     /locus_tag="ACHOQV_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFP9633201.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2086..2670
                     /gene="mobA"
                     /locus_tag="ACHOQV_004974"
     CDS             2086..2670
                     /gene="mobA"
                     /locus_tag="ACHOQV_004974"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFP9633202.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWIHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            2652..3179
                     /gene="mobB"
                     /locus_tag="ACHOQV_004975"
     CDS             2652..3179
                     /gene="mobB"
                     /locus_tag="ACHOQV_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFP9633203.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPVLCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALMDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(3282..3397)
                     /gene="rrf"
                     /locus_tag="ACHOQV_004976"
     rRNA            complement(3282..3397)
                     /gene="rrf"
                     /locus_tag="ACHOQV_004976"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          890 a          841 c          829 g          871 t
ORIGIN      
        1 gcccgtgaat attcacgggc tttatgtaat ttacattgaa ttattttttc tcggacagat
       61 atttcacagt atcagcatac tgctgaacaa aaacatccat attgctggta tccataccct
      121 gcggattcag ctgatattta ccgttaacaa acatcgccgg aacgccacgc aattgcacgt
      181 cagctgcagc tttttcctgc tgagcgacca gggatttcac cacgaagctg ttccacgctg
      241 cgtcgtactc ttcaccttta atacctgcgt tgataaatac atcgcggata tcagatgctg
      301 aacgaatggt ctgggttttc tgtacgcctt caaacagcgg aacagtgact ttgtcttcca
      361 cgcccagtgc catcgccact gcccatgcct gagtcagatc tttgcccagg tcacccccca
      421 tgaagttgac gtggtattta gtcatcttca cgccttccgg cagttttttc ttcacgttat
      481 cagaaatatg cagaacttct tcaaactgat agcagtgcgg gcagaagaaa gagaaaaact
      541 ccagcacttg cggcgcgcca gcaactggtt tttccagggt agtgtactgt ttaccatctt
      601 catactgcgc cgccgatgcg ctaaacgcta aaactaaacc agccagcgcc agccaaatct
      661 ttttcatgat ctactctctc cgattaatac ataggtgtta attgcaaagg gggttcttgt
      721 agaacttttg cctgttctat aaaagtcgcc gtctgtcgca gccagtaatc ttccccggtt
      781 aaccacggga aatttttcgg gaacgcggga tcagcccaac gccgcattag ccaggcaaga
      841 taataaacca aacgcatggc gcgtaaaggt tcaatcagtc cgatttcagc ggtgtcgaac
      901 tcgctaaatt cttcataagc ttcaataata gtttccagtt gcatccgctg ctcggcttta
      961 tcgccattga gcaacatcca caaatcctga atagccggac cattacgtgc atcatccaga
     1021 tcaacaaaca ttggaccatc gcgccagaga atattcccgg cgtggcagtc tccatgtagc
     1081 cgcagaacgg tgaaatcttc ccgccagtgt gctgtaacgg cggcgatcag ctcatctgtc
     1141 gctttcagaa atgccgcttt caacccggaa ggtatcagtg tagcgtcctc aaacagcttg
     1201 cgaggctcaa tgagatattc attcagaccg atggtcgggc gatggataaa aagctgtttg
     1261 cgccccgtct ggtgcatacg ccctaaataa cgcccaaccg cttccatctg atcgatatta
     1321 tcagcttcga actggcgacc accgacgctt ggaaaaacag cgaaataaaa tccctgatga
     1381 ttcaataaag tctgaccgtt aaaggccaca ggtgctgcga ccggaacttc atcatttacc
     1441 aactgcaacg caaattgatg ttcttcgagg atttgatcgg gtgtccaacg ttcaggacga
     1501 taaaatttga cgacaaaacg tcgacgatct tcgtcctgaa attgatagac acggttttca
     1561 tagctgttaa gcggggtaag accggaatcc acccggatcc cttgctcaaa cagagcatcc
     1621 atgatggtat ccgggtgtag tgtctggaaa gtaaaagcgc tgttgttcat cccatcatcc
     1681 ggaaaacatt acgaatgatg aaggatatca tttcacgatg attttggtgg caccgcttac
     1741 aagcttttac tctttaatta cgccgcgagc gcgtaacagc gccgttttga agtcttcttc
     1801 ataatctttc tgcaaacccg ggatcaccgc atctttcgcg gaatcacgca ttttcaggtg
     1861 atagatcaga atgtcatccg tcaaatccgc cagttcgccg tcaaaacctg actctttcgc
     1921 cagtttctgc aaaaattgca gcaggttaag gtctggctct ttttgccagg ctggctggag
     1981 gagttcaata acttcattca gacgtttaca tttcatagta atgctcctta ctcttgagac
     2041 agacacgtta gcagggtcaa tcccacaata aaagaggcga tatcggtgaa tctgatgacg
     2101 acgataacag gcgttgtgct ggcaggcggt aaagccagac gaatgggcgg cgtagataaa
     2161 ggattgcttg aattaaacgg caaaccatta tggcaacatg tcgctgacgc gcttatgacg
     2221 cagctctctc acgtcgtggt taatgctaat cgtcatcagg aaatctatca ggcaagcggt
     2281 ctgaaagtga ttgaagattc actggcggat tacccaggcc ctctggcagg aatgctttca
     2341 gtaatgcagc aggaagcggg tgagtggttt ttgttttgtc cgtgcgatac gccttacatt
     2401 ccccatgatt tagccgcccg gcttaatcat cagcgcaaag atgcgcctgt cgtgtggatc
     2461 cacgacggtg aacgcgatca cccgactatt gctctggtaa accgcgcaat tgagccttta
     2521 ttactggaat atctgcaagc aggagaacgc cgggttatgg catttatgcg tctggctggc
     2581 ggtcatgcgg ttgatttcag cgatcgtaaa gaggcattta taaacgtgaa tacgccagag
     2641 gagcttgccc gatggcagga aaaacgatga taccgttact cgcctttgcc gcgtggagtg
     2701 gtactggaaa aactacactg ctgaaaaaac tgatcccggt attatgcgcc agagggatcc
     2761 gtccgggact gattaagcat acgcaccatg atatggatgt tgataaaccg ggcaaagata
     2821 gctatgagtt gcgcaaggct ggcgcggcac aaaccatcgt tgccagccag caacgatggg
     2881 ccttgatgac ggaaacacca gacgaagaag agctggatct acattttctc gcaagtcgaa
     2941 tggatacctc aaagctggat ttgattctgg tcgaagggtt taagcatgaa gagatcgcaa
     3001 agattgtgct gtttcgcgat ggagccggac atcgaccgga agaattagtg atagacagac
     3061 atgttattgc tgtagccagt gatgtgccgc ttaatcttga tgtcgcgtta atggatatta
     3121 atgatgttga ggggctggct gattttgtgg tggagtggat gcaaaagcag gacggataag
     3181 caaaagtagg ctggataaga cgcgccagcg tcgcatccgg tatttattct gctccagcgc
     3241 aaaaaggcca tcctgacgga tagccttctg cttaatttga tgcctggcag ttccctactc
     3301 tcgcatgggg agaccccaca ctaccatcgg cgctacggcg tttcacttct gagttcggca
     3361 tggggtcagg tgggaccacc gcgctaaggc cgccaggcaa attctgtttt atcaacacgt
     3421 ctcgttatga c
//
LOCUS       DAZKGF010000302         3416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZKGF010000302 DAZKGF010000000
VERSION     DAZKGF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.302"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..183)
                     /locus_tag="ACHOQV_004977"
     CDS             complement(<1..183)
                     /locus_tag="ACHOQV_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633204.1"
                     /translation="MKYSVIAVIILFLLSSKSYAEEVSIDSGDPCTVFMCMAGKVYGE
                     NSSECKGPTKKFFNIIK"
     gene            complement(208..1836)
                     /locus_tag="ACHOQV_004978"
     CDS             complement(208..1836)
                     /locus_tag="ACHOQV_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFP9633205.1"
                     /translation="MGKQKIPDGWVLENGKLIQKRVTHYPPIIFGKDPYSNNLLVSYG
                     QTYLMLAAPPGSGKGVGIVIPNLLLYPHSVVVNDCKFENWDLTAGFRKACGQKVVRFS
                     PERLETHHWNPLKVINQDPLYRLGDVRTLASSLYVPDNMKNASWFSKAADVFTAIVLY
                     LIETPSLPLSLPQCYEIASLGTRIGEWAQRVIQARVGTPDALSEETVRELGVIISESK
                     GREFSTLMSFVTGRLSLYGEKLVALALTESDNEEENIDFSKLREEPTSIYFCVTEGAL
                     QKFGPLMNLFFSQAIRENSKILPEQGGNLEDGSLRLKYQVLFLEDEFAVMGRIKVMET
                     APALSRGAGLRYIIVFQNKSQVCSDDCYGPAGGKAVMDPFHIEVVFAPGDISTAEEYS
                     KRLGTYTERVASSSRNISEGQRRSRGRNVTLHARPLMLPQEVNELPYPEELVFMQPTK
                     WNPALKGRVKKIMWYKEPELTARVDPVRYPVPDIPAGNISKLKGLVVPISQYITDAVK
                     ISSPEGDYLNEEQKKRLKIPQVESERESYEDNDY"
     gene            complement(1836..2198)
                     /locus_tag="ACHOQV_004979"
     CDS             complement(1836..2198)
                     /locus_tag="ACHOQV_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633206.1"
                     /translation="MEKLKLFIVIIALCCLFFVIGLYAGGWMWLELSGVDSSPSLMTL
                     ISQGGLPALNDRARMLLPWAWCVTVAITFFPAGVSLLAMTHGADGNRKDLHGNARFAG
                     KRELRRFWYEGPEKEDRN"
     gene            complement(2210..3259)
                     /gene="virB11"
                     /locus_tag="ACHOQV_004980"
     CDS             complement(2210..3259)
                     /gene="virB11"
                     /locus_tag="ACHOQV_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFP9633207.1"
                     /translation="MNTEFDLDKSELVLGWLDQSGVTERLSRQGVTEVAINRPFEIWT
                     ETSKDGWQREDAPWLTLNMCEHLAETLAVYNNRILGVKSPIKTVELPGGERGQIVIPP
                     ACEKSTVSMTFRKPSLQRFSLENYDSSGRFDNANRVRGRSKELLDWQKELKRLNAARE
                     YVEFFRLAVRHKLNIIIFGGTGSGKTTFAKAIVDVYPSNRRLLTIEELNEMKLPYHLN
                     HVHLLYGDYVTPKDLVACCMRMKGDHILLAELTGDETWDFMEVLNTGHPGTVTTAHAN
                     SSLHGYARISGLIKQSAVGAGLDISYIDRRVRTSFDVVMYMEKTEILDITYEPEVQLA
                     LLNGASMESALSFAS"
BASE COUNT          967 a          865 c          583 g         1001 t
ORIGIN      
        1 ttttataata ttaaagaatt tttttgttgg tcccttacat tcagatgaat tttccccata
       61 aacttttcca gccatacaca taaagacagt acagggatcc cccgaatcta tacttacctc
      121 ttcagcataa cttttactgg ataacaggaa aagaataatc accgctatga cagaatattt
      181 catattaacc ccctttttat taaaaattca gtaatcatta tcttcataag attccctttc
      241 tgattccact tgtgggattt ttaatcgttt tttctgctcc tcatttaaat aatcaccttc
      301 tggtgaagat atcttaactg catccgtaat atactgactt attggtacaa caagtccttt
      361 aagtttcgat atattcccgg ctggtatatc aggcacggga taacgaacag gatccactct
      421 tgctgttaat tcaggttcct tgtaccacat tatttttttc acccggcctt tcaaggcggg
      481 attccatttt gttggctgca taaacaccag ttcttcagga tatggaagtt cattaacctc
      541 ctgtggcaac attaaaggcc tagcatgcag tgtaacatta cgtcctcgag aacgacgctg
      601 tccttccgat atattcctgc tactggacgc aactcgttct gtgtacgttc ccagtcgttt
      661 actgtactcc tccgctgtac tgatatctcc gggggcaaat acaacctcaa tatggaatgg
      721 atccattaca gctttccccc ctgcgggtcc ataacagtca tcagagcaaa cctgactttt
      781 attctgaaaa acaattatat agcgtaatcc tgcccccctg cttagtgccg gggctgtttc
      841 cataaccttg atacggccca tcacagcaaa ttcatcttcc agaaaaagca cctgatattt
      901 caatctgaga gaaccgtcct ccagatttcc cccttgctcc ggtagtattt tactgttttc
      961 ccttattgcc tgagaaaaaa acaaattcat tagtggacca aatttctgta aagctccctc
     1021 ggtaacacaa aaataaatgc tggttggttc ttcccttaac ttgctgaagt caatattttc
     1081 ttcctcgtta tcactctccg tcagcgccag cgccaccagt ttttcgccgt acaagcttag
     1141 acgtcccgtg acgaatgaca tcaacgttga aaattccctg cctttcgact cggaaataat
     1201 cacccccaac tctcggacgg tttcttctga cagtgcatct ggcgttccta cacgagcctg
     1261 aataacacgc tgcgcccatt ctccaatacg agttcccaac gatgcaattt cgtaacattg
     1321 cggtaaactc aatggcaacg atggtgtttc gatcaggtat aaaactatcg cggtaaacac
     1381 atctgcggct ttagaaaacc aactggcatt cttcatgtta tcaggtacat acagagatga
     1441 ggctagtgtt cgaacatctc ccaagcgata cagcggatcc tgatttatta cctttaacgg
     1501 attccagtga tgggtttcca gtcgctctgg cgaaaaacgc acgactttct gaccacatgc
     1561 ttttctgaaa cccgctgtca aatcccagtt ttcaaattta cagtcattca ctacaacaga
     1621 atgcggatac aatagtagat tagggatcac aatccccacc cctttaccac ttcccggtgg
     1681 agcggccaac atcagatagg tctgtccata ggatacaagt aaattattac tgtacggatc
     1741 tttaccaaaa ataattggtg gataatgagt tactcttttt tggattaact ttccattttc
     1801 cagcacccat ccgtctggta ttttttgttt tcccattaat ttctgtcctc cttctctggc
     1861 ccttcatacc agaaacgacg caattcccgt tttccggcaa acctagcatt accatgtagg
     1921 tcctttctgt tgccgtccgc tccatgagtc atggccaaaa gagaaacacc tgccgggaaa
     1981 aaggtaattg caactgtcac acaccacgcc cagggaagta acattctggc ccgatcattc
     2041 agtgctggca gtcctccctg acttatcagg gtcatcaaag atggagagga gtcaacacct
     2101 gataattcaa gccacatcca tcctccagcg taaagcccaa taacaaaaaa caaacaacaa
     2161 agagctatta tcacgataaa tagtttgagt ttttccatac agcactacct cacgatgcaa
     2221 aagaaagagc actttccatg ctggcaccat tcagtaatgc cagttgcacc tctggctcat
     2281 acgtaatatc caagatttct gttttctcca tatacatcac aacatcgaaa gacgttctga
     2341 cacgtcgatc gatataagag atatccagcc cggcaccaac cgcagactgc tttattagtc
     2401 cagagatacg agcataacca tgtaaactgc tattagcatg cgctgtcgta accgttcctg
     2461 gatgcccggt attcagcact tccataaaat cccaagtttc atcgcctgtc agttctgcca
     2521 gaagaatatg atcccccttc atgcgcatac aacacgctac caggtctttt ggtgtcacat
     2581 aatctccata caataaatgt acgtgattaa gatgataagg cagcttcatt tcattcaatt
     2641 cttcaatagt cagtaaccga cgatttgacg ggtaaacatc aacaattgct ttagcaaaag
     2701 ttgtttttcc cgaaccggtt ccaccaaaaa taataatatt tagtttatgt ctcacagcca
     2761 gacggaaaaa ttcaacatat tcacgagcag cattaagacg ttttaattcc ttttgccagt
     2821 ccagtaactc tttacttctt cctcttacac gattggcatt atcgaaacgt ccactggagt
     2881 cataattctc gagagagaaa cgttgaagag acggtttacg aaacgtcatc gacacagtag
     2941 atttctcaca ggcgggagga ataacaattt gtcctcgttc gcctcctggt agttccactg
     3001 tttttatcgg tgacttaaca cctaatatac ggttattata aacagccagt gtttcagcca
     3061 ggtgttcaca catattcagc gttaaccacg gtgcatcttc cctttgccag ccatcttttg
     3121 atgtctcggt ccagatctca aaaggtctgt taatcgctac ctccgtcact ccctgacggg
     3181 acagacgttc tgtaacacca gactgatcca gccaccccaa aacaagttct gacttatcga
     3241 ggtcaaattc ggtattcatt aatagacgcc ctttttattt acacttttat gagttacttt
     3301 tctatctcat taatgaaatg ataaatgcag caacgatcat tatgattgac caccgtaaat
     3361 ttattgggat acgataaaaa taaatacaca cccatttaaa aaatcccatc caacct
//
LOCUS       DAZKGF010000303         3386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZKGF010000303 DAZKGF010000000
VERSION     DAZKGF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.303"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            120..1085
                     /gene="dusB"
                     /locus_tag="ACHOQV_004981"
     CDS             120..1085
                     /gene="dusB"
                     /locus_tag="ACHOQV_004981"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP9633208.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1111..1407
                     /gene="fis"
                     /locus_tag="ACHOQV_004982"
     CDS             1111..1407
                     /gene="fis"
                     /locus_tag="ACHOQV_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFP9633209.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1493..2377
                     /gene="yhdJ"
                     /locus_tag="ACHOQV_004983"
     CDS             1493..2377
                     /gene="yhdJ"
                     /locus_tag="ACHOQV_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP9633210.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAVLVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVANGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2461..2640
                     /locus_tag="ACHOQV_004984"
     CDS             2461..2640
                     /locus_tag="ACHOQV_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFP9633211.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2643..3305)
                     /gene="acrS"
                     /locus_tag="ACHOQV_004985"
     CDS             complement(2643..3305)
                     /gene="acrS"
                     /locus_tag="ACHOQV_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFP9633212.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
BASE COUNT          932 a          783 c          801 g          870 t
ORIGIN      
        1 ttttaaatgc aattctttga tccatctcag aggattggtc aaagtttggc ctttcatctc
       61 gtgcaaaaaa tgcgtaatat acgccgcctt gcagtcacag tatggtcatt tcttaactca
      121 tgcgcatcgg acaatatcag ctcagaaatc gcctgatcgc agcgcccatg gctggcatta
      181 cagacagacc ttttcggacg ttgtgctacg agatgggagc cggattgaca gtatccgaga
      241 tgatgtcttc taaccctcag gtttgggaaa gcgacaaatc tcgtttacgg atggtgcaca
      301 ttgatgaacc cggtattcgc accgtgcaaa ttgctggtag cgatccgaaa gaaatggcag
      361 atgcagcacg tattaacgtg gaaagcggtg cccagattat tgatatcaat atgggttgcc
      421 cggctaaaaa agtgaatcgc aagctcgcag gttcagccct cttgcagtac ccggatgtcg
      481 ttaaatcgat ccttaccgag gtcgtcaatg cagtggacgt tcctgttacc ctgaaaattc
      541 gcactggctg ggcaccggaa caccgtaact gcgaagagat tgcccaactg gctgaagact
      601 gtggcattca ggctctgacc attcatggcc gtacacgcgc ctgtttgttc aatggagaag
      661 ctgagtacga cagtattcgg gcagttaagc agaaagtttc cattccggtt atcgcgaatg
      721 gcgacattac tgacccgctt aaagccagag ctgtgctcga ctatacaggg gcggatgccc
      781 tgatgatagg ccgcgcagct cagggaagac cctggatctt tcgggaaatc cagcattatc
      841 tggacactgg ggagttgctg cccccgctgc ctttggcaga ggttaagcgc ttgctttgcg
      901 cgcacgttcg ggaactgcat gacttttatg gtccggcaaa agggtaccga attgcacgta
      961 aacacgtttc ctggtatctc caggaacacg ctccaaatga ccagtttcgg cgcacattca
     1021 acgctattga ggatgccagc gaacagctgg aggcgttgga ggcatacttc gaaaattttg
     1081 cgtaaacaga aataaagagc tgacagaact atgttcgaac aacgcgtaaa ttctgacgta
     1141 ctgaccgttt ctaccgttaa ctctcaggat caggtaaccc aaaaacccct gcgtgactcg
     1201 gttaaacagg cactgaagaa ctattttgct caactgaatg gtcaggatgt gaatgacctc
     1261 tatgagctgg tactggctga agtagaacag cccctgttgg acatggtgat gcaatacacc
     1321 cgtggtaacc agacccgtgc tgcgctgatg atgggcatca accgtggtac gctgcgtaaa
     1381 aaattgaaaa aatacggcat gaactaattc aggttagcta aatgcttgat taaaaaggcg
     1441 ctactcggca tggggaagcg ccttttttat aggtgtcaca aagggagtga ccatgagaac
     1501 aggatgtgaa ccgacccgat ttggtaatga agcaaagacc atcatccagg gtgatgcatt
     1561 aaccgaactt aaaaagctcc ccgccgaaag cgtcgatctg atctttgccg acccaccgta
     1621 taacatcggt aaaaattttg atggtctgat cgaagcctgg aaagaagatc tgtttatcga
     1681 ctggctgttt gaagtgattg cagagtgcca ccgcgttctg aaaaagcagg gcagcatgta
     1741 catcatgaac agcacggaaa acatgccctt tatcgatctc cagtgccgca agctttttac
     1801 catcaaaagt cgcatcgtct ggtcatatga cagttcagga gtgcaggcga aaaaacacta
     1861 cggctccatg tacgaaccca tcctgatgat ggtgaaagac gcaaagaact acacattcaa
     1921 cggtgatgct gttctggtag aagccaaaac cggatcgcag cgcgcgttga tcgattatcg
     1981 caaaaatcct ccacagcctt acaatcatca aaaagtaccg ggtaacgtct gggattttcc
     2041 tcgcgtgcgt tatttgatgg atgaatatga aaaccacccg acgcaaaaac cggaagcctt
     2101 actgaaacgc attattctcg cctcttccaa cccaggcgat atcgttctcg acccgtttgc
     2161 cggtagcttt actaccggtg ccgtagccgt cgccaacggg cgaaaattca ttggtattga
     2221 gatcaacagc gagtacatca aaatggggct tcgacggctg gatgtcgcgt cgcattactc
     2281 tgcggaagaa ctggcgaaag tgaaaaaaag aaagaccggc aacctgtcaa aacgaagccg
     2341 gttaagcgaa gttgaccccg atctcattgc aaagtaaagg aagtgtaagc ctggttttca
     2401 gattattcat ttcgtgtata tttcctgcca gacttggtta aacatgcaca ggctcaggta
     2461 atgattcgca agtattggtg gctcgtcgtt ttcgctgtct tcgtttttct gtttgatact
     2521 ttactgatgc agtggattga actgctggca acagaaacag acaaatgccg caatatgaac
     2581 tcagttaatc cactaaaact ggttaactgt gacgaactga attttcagga cagaatgtga
     2641 atttatatga cacttaattc attcgtttta tgaattaatt tcgttatgtt ttcatctggc
     2701 atgaacattc ttaatacgtt atcgaccaga gcgggggctt gtttataaag atcataaccc
     2761 gccatattca ttaaccagtt ttgaacaatt ccgctgaagg caccatcaat aataatcatc
     2821 acaacatcta aatcgagatt atttgctaca caaccttgtt gctgacacgc ctgcaatact
     2881 tcgcggagag tctgcggatt aaagcccatc ttttcgcgta tcactccctc ggccagcatc
     2941 tcatcattaa attcacattt gtgatataag attttcagca acgcctgctg gcggggaatt
     3001 ttggcaatat attgcaaacc gacaatcaat ttttcacgca attgctgaaa cgggtcatgc
     3061 tctaacccag cggtcaagtg atcctggatt aactcccgca atgaaggctg ttgcaaccac
     3121 atctcattaa acagttgggt cttgttctcg aagtgccagt agatagcgcc acgcgtaacg
     3181 ttagcagcgt cggcaatgtc gttgagcgtc gtcttgctta cgccatgttg cgcaaactgg
     3241 gcgatggcag tttcaatcag ttcttgccgg gtcttcagag cttcggcttt ggttcttttt
     3301 gccatgatta attattcagg aaataaatat attcgacaca gagtgagaaa atagcgaagg
     3361 ttaatctatc acctaatgtg tattta
//
LOCUS       DAZKGF010000304         3383 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZKGF010000304 DAZKGF010000000
VERSION     DAZKGF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.304"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..115
                     /locus_tag="ACHOQV_004986"
     CDS             11..115
                     /locus_tag="ACHOQV_004986"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9633213.1"
                     /translation="MFTVTAATEREARARLPHAHLIFVARIRQEVTHA"
     gene            108..293
                     /locus_tag="ACHOQV_004987"
     CDS             108..293
                     /locus_tag="ACHOQV_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633214.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            297..506
                     /locus_tag="ACHOQV_004988"
     CDS             297..506
                     /locus_tag="ACHOQV_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633215.1"
                     /translation="MCDFSDMTVDEAAKSAMQAELICSLMMGNTGGMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDDAICVND"
     gene            551..742
                     /locus_tag="ACHOQV_004989"
     CDS             551..742
                     /locus_tag="ACHOQV_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633216.1"
                     /translation="MQQITLAEASLQAQQVEVISRMIERNHEEIDDHDLSVIAGLIKQ
                     LSSNVAVWLMTEEEKRGEQ"
     gene            919..3060
                     /locus_tag="ACHOQV_004990"
     CDS             919..3060
                     /locus_tag="ACHOQV_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFP9633217.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWQEQDGKLA
                     GDKTPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHNERQNWRNYLETERKNISDGLVIELPVKKKTSEQSEDELKPRVECRPDG
                     VYWVTPKVDKQSGEIIRPETWLCASVELLGAGVIGNEHYRVMRWTDSTTNRLVTMAVP
                     CCGIGDSDGWRLLKANGLKVTTNGKHRIPLADWMQLDGQHEEWHLSTKAGWHFGAYIM
                     PDGTVIGESDKPVLFTGKSAAINGYSVAGTADSWRDSVARLAGGNPFMMLGIATSLSA
                     PLVGLVGADGFGVHFFENSTAGKTTTQSVASSLWGDPEAQRLTWYGTTLGIANEAEAH
                     NHGLLPLDEIGQAASARDVYVSAYTLFNGFGKLQGAKDGGNRELKSWRTVAISTGEVD
                     IETFLKTEGIKVKVGQLVRLLNVPMEKATKFHEYSNGKEHADALKEAWKENHGAAGRE
                     WVKWLSGHQQEAKDTVRECRERWRNLIPESYGEQVHRVGERFAILEAALVLSGHVTGW
                     AVQECRDAILHNFNAWVKVFGTGNKEHKQIIEQAEAFLAAYGMSRFAPVNYDPASLPI
                     PELYGYRESDGRYDEPVLFYVLPDPFGSHVANGFNKDAVAKVLHEAGMLKRPSSGRGW
                     QIRTPRLKHLKGARLRVYGLLLAQDHDTESD"
BASE COUNT          950 a          744 c          956 g          733 t
ORIGIN      
        1 cagcattgca atattcaccg ttaccgctgc aactgagcgc gaagcacgcg cccggctgcc
       61 gcacgcacat ctgatttttg tcgcccgaat ccgtcaggag gtgactcatg cttaaaacct
      121 tccgcgtctt ttcccgtgct gttaacaagc gtggctatac cgttggcatc acgcagaacg
      181 tgaaggccga aaacgtccag gctgcaatca gcacagtaat taacaaatca gctaaagccg
      241 gtttatccgg cgtaattatc cacgcagtga catgtattca ggatgaggag taattcatgt
      301 gtgatttttc cgatatgact gttgatgaag cagcaaaaag cgcaatgcag gcagaactta
      361 tttgttctct gatgatgggt aacacaggtg gaatgactga gggtgagatt gagtcattgc
      421 tcgccctgat taagcagctt acaggtaagg caggtagctg gctgattgct gcatcgggtg
      481 atgatgcaat atgcgtcaat gactgaatcc tttgttgttc tgaatatcat gaaattaagg
      541 gggtgcagta atgcagcaaa taacactggc agaagcctca ctgcaagcgc aacaggtcga
      601 agtaatcagc cgcatgattg aacgcaacca cgaagaaata gacgaccatg acctttctgt
      661 tattgcagga cttatcaaac aactttccag caatgtggcg gtgtggttaa tgacggagga
      721 agaaaaacgc ggcgaacaat aacggctaaa aataaacggg aaataaagat taagacgact
      781 ccttcaggag attaattcaa tggcatcaaa aaatactggc ttaactgcca gtagcaaaac
      841 tcaccccgaa tttatgtgcg gggatattta ccgcaatgaa tacggtagcc acatgacgat
      901 gatcagcaga ggtaagaaat gaaaagcgca ccgaacttaa aaaaacagcc tcgagggaaa
      961 aaacacgccg atacggaagt gattattttc gctggcagtg atgcgtgggc gcacgcgaaa
     1021 cagtggcagg agcaggacgg aaaacttgcc ggagataaaa caccgcctgt cgtgctggct
     1081 gatgagcaac ttaaagatat tggcaatctg caaattgtgc ccgacgggcg caaatcagcg
     1141 cggatataca aggccggaca ccttgatcag gtgatggtca aaggcatagg tcagaagctg
     1201 gcggcggcag gcgtacagga cgcagactat taccccgaag gtatgcacca taacgagcgg
     1261 cagaactggc gtaactatct ggagactgag cgcaaaaaca tttctgacgg actggttatt
     1321 gagctgcctg taaagaaaaa aacatcagaa caatcagagg atgaattaaa accccgcgtt
     1381 gaatgccgtc ctgatggcgt ttattgggtt acgccaaagg ttgataagca atcaggcgaa
     1441 attatacggc ctgaaacgtg gttatgtgct tcggttgaat tactgggcgc tggtgtcatt
     1501 ggtaacgagc attaccgcgt gatgcgctgg acagattcga caacaaatcg gcttgtcact
     1561 atggccgtgc cttgctgcgg aataggcgac agtgacggct ggcggttact gaaagccaac
     1621 gggcttaaag tcacgacaaa cggaaaacac aggatccccc tggctgactg gatgcagtta
     1681 gacggacagc atgaggaatg gcacttaagc acgaaagctg gctggcactt tggcgcgtac
     1741 atcatgccgg acggtacggt tatcggcgaa tctgacaagc ccgttttgtt taccggaaaa
     1801 agtgctgcca ttaacggtta ttccgtggcg ggtacggcgg atagctggcg cgacagcgtg
     1861 gcgcggctgg ctggtggtaa tccgttcatg atgctgggca tagcgacgtc attatccgcg
     1921 ccattagtcg ggctggtcgg tgctgatggc tttggtgtgc attttttcga aaactccaca
     1981 gcaggtaaga ccacaacaca aagcgtggca tcgagtttat ggggtgatcc tgaagcgcag
     2041 cgattaacct ggtacggaac aacgttaggc attgctaacg aggctgaagc acacaaccac
     2101 gggcttttac cactggatga gataggacag gcagcaagcg cccgtgatgt gtacgtatcg
     2161 gcttatacgc tgtttaacgg cttcgggaaa ttacaggggg caaaagacgg cggaaacagg
     2221 gagctgaaaa gctggcgcac agtcgctatc agcaccggag aagtggacat tgaaacattc
     2281 ctcaaaaccg aggggataaa ggtaaaagta gggcagcttg tccgcctgct taatgtaccg
     2341 atggagaaag ccacgaaatt tcacgaatac agcaacggta aggaacatgc ggacgcgtta
     2401 aaagaagcct ggaaggaaaa ccacggggcg gcgggtcgtg agtgggttaa atggctgtca
     2461 ggccaccagc aggaggcaaa ggatacggta agggaatgcc gcgaacggtg gcgcaacctg
     2521 ataccggaga gctacggcga gcaggtccac cgcgtgggtg aacgtttcgc catactggag
     2581 gccgcgcttg tgctttccgg tcatgtaact ggctgggccg tgcaggaatg ccgcgacgcg
     2641 atactgcaca actttaatgc ctgggtgaag gtgttcggta cggggaacaa ggagcacaag
     2701 cagattattg aacaggctga ggcgtttctt gcggcctatg ggatgagcag gtttgcccct
     2761 gtcaattatg accccgccag tctgccgatt cctgaactgt acggctacag ggaaagcgac
     2821 gggcgttatg acgaacctgt tttgttctac gtgctgccgg atccgttcgg ctcacatgtg
     2881 gcgaatggat ttaacaagga tgccgtggca aaagttcttc atgaagccgg aatgctgaaa
     2941 agaccatcca gcggtcgtgg gtggcaaatc aggacaccac ggctaaaaca cctgaaaggg
     3001 gcgcgcctga gagtgtacgg ccttttgctg gcgcaggacc acgacacgga aagcgattaa
     3061 tctcgcgtat aggcgatgaa aaaatgttgt cccagttgtc ccactacgta ttaattaatt
     3121 gattttacta aaaatacaat aaaaaaagtg ggacaacatt acagcatttt gggacgacat
     3181 cggggtattt tgggacgaca tagcgacatt ttgggacgac acaacgagcg ttaattaccc
     3241 tggctggtga ctgggacaac ataaaacccc ttatgttgtc ccaaaatcgg ctaatgttgt
     3301 cccagttttc ggagtgtatt tttaattaaa tcaatggatt atgtgtaagt gggacaactg
     3361 ggacaactgg gacgacgtgt ttt
//
LOCUS       DAZKGF010000305         3304 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZKGF010000305 DAZKGF010000000
VERSION     DAZKGF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.305"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            112..603
                     /locus_tag="ACHOQV_004991"
     CDS             112..603
                     /locus_tag="ACHOQV_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9633218.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     ASSKNEEGSQTASLQMRHEKEGHLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            606..1874
                     /locus_tag="ACHOQV_004992"
     CDS             606..1874
                     /locus_tag="ACHOQV_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFP9633219.1"
                     /translation="MALIGVYADWEGLDGPERIGYLHSRRTRTREIFEFEYDKKALAD
                     PSLNFIQLDPEIMLYEGAQYPIPPKDKFGAFSDSCPDRWGRMLMKRRFERDIRDGLCD
                     KDSHLYESDYLLGVHDLYRVGALRYKREDAGEFLDNRIDVAAPPFTEIASLERASRAI
                     EEDPDNKELMGQKWLRMLIAPGGSLGGARPKASVVDEAGHLYIAKFPSVKDEYDVGGW
                     EMAVNALAVGCGLNVAPAQAHKFASNYHCFMVRRFDRTNAGRRLHFASAMTLTRHQDG
                     EDASTGVSYLELADVLIRHGAQTNTDLKELWSRIVFNILVSNTDDHLRNHGFILIPGK
                     GWRLSEAYDLNPVVRSDGLKLNITENDNALDLELAREVAEYFRLSLTEADDIIANFRG
                     IVSQWRIIAERLRLPGREQELMAEAFRASI"
     gene            complement(2017..3294)
                     /locus_tag="ACHOQV_004993"
     CDS             complement(2017..3294)
                     /locus_tag="ACHOQV_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9633220.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPIMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWMLIKVKDEEPQVQLATS"
BASE COUNT          958 a          678 c          785 g          883 t
ORIGIN      
        1 aagataaaat accatcactt tcgtaatctt gaacgctttg caggtgcgtt caatccaata
       61 aatgctaata ttatggctat catagccata aaaagataaa atattatcac tatgggtaaa
      121 aaaacagcac cgttactccc ttatacgaat cagatgctta gtgaatttgg tgagcggctg
      181 aaacttgctc gtttacgacg caggcttacc gcaaagcaag ttgctgaacg tgctgggatg
      241 tcgccaataa cattgcgctc tctggagtca ggaagtgcag gggtcacgat aggggcatat
      301 ttgtctgtga tgcaggtttt gggcctggaa acggatttaa ataagcttgc agctgaagac
      361 gacttagggc gtcagctcca ggattcccgt ttgattaaaa aaagagggct ttctcgtgca
      421 tcaagtaaga atgaagaagg ttctcagaca gcctcattgc agatgaggca cgagaaagaa
      481 ggtcatctca ccacggattt aacagcactg gcagaaggct cgacgccatc tattacagga
      541 gaaacatcaa cctctcttgc agcactatta aaaccgataa aaaaaccgtt aaaaggaaat
      601 taattatggc attaatcgga gtgtatgcgg attgggaagg cctggatggt ccggagcgga
      661 ttggttattt acacagtcgc cgcactcgga cgcgtgagat atttgagttt gagtacgata
      721 aaaaggcgct tgcagatcct tcacttaatt tcattcagtt agatcctgaa ataatgttgt
      781 atgagggagc acagtaccca attcctccaa aggataaatt tggtgcattt agtgattcct
      841 gcccggatcg ttgggggcgt atgctaatga agcgtcgttt tgagagggat attcgagacg
      901 ggttatgtga taaagactcc cacctttatg agtctgatta tctactaggt gttcatgatc
      961 tttatcgggt tggggccctt cgatacaaac gtgaagacgc tggagagttt cttgataatc
     1021 gcattgatgt tgcagcccca ccatttaccg aaatagcgag tctggaaagg gctagccggg
     1081 caattgaaga agatcctgat aataaagagc taatggggca aaaatggtta agaatgctaa
     1141 tcgctccggg gggatcatta gggggggcac gtccgaaagc cagtgttgtt gatgaagctg
     1201 ggcatctcta catcgctaaa tttcccagtg taaaagatga gtatgatgtt ggtggatggg
     1261 aaatggccgt taatgcactt gctgttggct gtggactgaa tgttgcacct gctcaggcgc
     1321 ataaatttgc cagcaattac cattgtttta tggttcgtcg ttttgaccgc actaatgcgg
     1381 gaaggcgtct gcattttgct tcggctatga cactaaccag gcatcaggat ggtgaagatg
     1441 cctctacagg tgtgagttat ctggaattag cagatgttct tatcaggcat ggcgcgcaga
     1501 ctaatactga tcttaaggaa ctctggtcca ggattgtttt taatattctt gtttctaata
     1561 ccgatgatca tttacgcaat catggattta tcttgatacc aggaaaaggt tggcggcttt
     1621 ctgaggcata tgatttgaat cctgtggtca ggtctgatgg tttaaaactc aatattacgg
     1681 aaaatgacaa tgcacttgat ttggaattag ctagagaagt tgcagagtat tttcgactta
     1741 gtttgacgga agctgatgac attattgcta attttagagg gattgtcagt cagtggcgaa
     1801 tcattgccga gcgtctgaga ttacctggtc gtgagcaaga gttaatggca gaggcattca
     1861 gagcgtcaat ttagaatagt tatttcgata tattcgctaa atcataaatt tgcgaagcaa
     1921 tgccagtaga tactgaacgt tttggaagag acgtttaatt tatgagaaaa atagaaacgc
     1981 tacaaagcta aattatagcg tttctatgta aaataattag cttgtcgcta actgtacttg
     2041 tggttcttcg tctttaactt tgataagcat ccacagcgca atggcgccaa taatgtcaaa
     2101 gaaagccagg gcaataaaga acggcgcgaa gccaataatg tgaacgaatg ccccgataaa
     2161 caggttgaaa atcaactgac cgctccatgc acaggctcct gccataccca cagcggtagc
     2221 tacttcgtct tttttgaata ggtcaccgcc cagagttgct gcaacagtag aaagacattg
     2281 atgcgagaat gcgccaatgc taatcagtaa tacggcgatg tagggatttt cgacaatact
     2341 caccagacca atcgtcatca taatgacggc tgcaatagtg aaggtaattc ttcgtgagtt
     2401 aattaaactc acgccgcgat cgtggaagaa cttcgcgaca aaaccactgg ctaaacagcc
     2461 gagatcgccc agcaggaaag gtagccagac gaacatggca atttctttca ggctaaaatg
     2521 cagcgtttcg acgaagaaaa tcggtaccca gaagttaatg gttccccatg ccgggtcagc
     2581 gagaaaacgc gcgatgccaa tcccccagaa attacgttgg ctgaggatat gcttgattga
     2641 cgttttttct ttcttatctg ttttaagatg ctgttcttgc ccagactcaa tatagtggcg
     2701 ctcttcatca gaaaggcgtt tagcatcttt tggatcttta taacaaaaga accaaaataa
     2761 agccgcgagt aaagcaaggc tacctgagac aataaacgca aattgccagc tatgaaacat
     2821 aatgcaccag gcaatcaacg gtggagcaag catcgcgccg agtgaggtgc ccatattgaa
     2881 aacacctgtc gccacgccac gctcttttgc cgggaaccat gttgatgcgg tttttacacc
     2941 cgcaggaatt gccgatgctt cgctgaaacc catcagggcg cgcataaatg ccagacctga
     3001 ccatgttcct gtcaaggcat gccccattgt cgccaggccc cagattgcgg cacatatcgc
     3061 aaaacttaac ttcagaccca cggtatcaat aaagaagccc attatcggtt gccccaatgt
     3121 ataggccaac tggaatgcac tgacaatcca tgaatattgt tctttggtta ttccggttgt
     3181 ctccataatc gttggagccg caatacctaa cgccgttctg tccagataat tgattacagt
     3241 gataaagcat acgagggcaa tcatatgcca tcgataattc tttattttat tcatttttaa
     3301 acct
//
LOCUS       DAZKGF010000306         3298 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZKGF010000306 DAZKGF010000000
VERSION     DAZKGF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.306"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..983
                     /locus_tag="ACHOQV_004994"
     CDS             <1..983
                     /locus_tag="ACHOQV_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HFP9633221.1"
                     /translation="RSWLARVTQMRLKLQQVTSAPDPQAMSQMLARAVFQGKAIDLTD
                     TCDYGSLVAASLGQEWSGFGQSLFVQPLDLAWRQVLVPAAGSLNARWQTTIVDQWNKA
                     FAGRYPFKATGSDASLPLLAQFLRSDSGRITTFLKTHLGGLVHQEGNHWVAEPSASQG
                     MVVSPDFLRAINQLAELSDIVFSQGDAGVHFELMARPSRDVARVQLTLDEQNLDYFNQ
                     MESWQSFTWPGNTYYPGVSLSWRSVSSGMQLYASHQGNWGFIRLLDEALITPLDASRT
                     QLVWITADGNPLKFVMRSELGDGPLALLKLQGFTLPTAIFNVAAGTEGVE"
     gene            1105..1272
                     /locus_tag="ACHOQV_004995"
     CDS             1105..1272
                     /locus_tag="ACHOQV_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633222.1"
                     /translation="MSEKTTDKLTEGQTHSAGERMLLKWRPPQIFNSKDDASPEAVVR
                     FMKETDKQEKE"
     gene            1269..2882
                     /gene="tssA"
                     /locus_tag="ACHOQV_004996"
     CDS             1269..2882
                     /gene="tssA"
                     /locus_tag="ACHOQV_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP9633223.1"
                     /translation="MSSPEALLTACAASQEERLSLISRANEALSLWDNWLHPFTSGSE
                     TGDDPAYDDNFQLMREEINKISGTDSALLCELAQKCLCECARDIRVVTWYVLARLTRD
                     GERGLSEGLLLLVAMLTRYGQACHPRRPAARKAALEWLNSAKVTDALLLWPGVDSNEA
                     GLTAGAISLLGSAVADWPEAEKPSFAGLCSALENRLARSGGMEALVPQNSSPRETGRE
                     SAPPDSPQLAAVTSGRDLLDQARLLSRWLSGQPQGWLASHRLMKTVRWDTVDQLPSLD
                     SSGRTRLVPPKTGYRAQLKRLWLQKNWVELIELASQMYCEGVNHFWLDLQWYLWQGLS
                     HAGQPWDSWSDAVLSDLRLLLKRLPGLEGLAWNDGTPFADEVTLAWIAEKVNEEDVMY
                     GDEAAIPAVVNGQTDDVLILESEAMEKGDTEGPEAALAWLQSRPGMDTPRHRWLIRLL
                     MARVAEQYGRNDMALHLLGELITRAPQLTLEDWEPALLFEVRARRLKLLRMKAGRSES
                     DKARLMPEMDTLLAGLIAIDPARAMVLCG"
     gene            2916..>3298
                     /locus_tag="ACHOQV_004997"
     CDS             2916..>3298
                     /locus_tag="ACHOQV_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP9633224.1"
                     /translation="MDDLTLRYYEAEMRYLREAGKEFARAHPDRAAMLNLDKPGACDP
                     GVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYMRTIPSLAIVEFSPDWRSL
                     RQAEILAEGFSVLSRPVGPHKTACQ"
BASE COUNT          668 a          886 c         1012 g          732 t
ORIGIN      
        1 tccggtcctg gctcgcccgt gtaacccaga tgcggctgaa acttcagcag gttaccagcg
       61 ccccggatcc gcaggcgatg tcgcagatgc tggcccgggc cgtgtttcag ggcaaagcca
      121 tcgatctgac ggacacctgc gattacggca gcctggttgc agcaagtctg gggcaggaat
      181 ggagtggatt tggtcagtcg ttgtttgtgc aaccgctgga tctggcctgg cggcaggtgc
      241 ttgtcccggc tgcgggcagc ctcaatgccc gctggcagac aaccatagtc gatcagtgga
      301 ataaggcttt tgcggggcgg tacccgttta aagccaccgg cagtgatgcc tccctgccgt
      361 tactggcaca gttcctgcgt agcgactcgg gtcggatcac gacgttcctg aaaactcatc
      421 ttggcgggct cgtccaccag gaggggaatc actgggtcgc tgagccttcc gcaagccagg
      481 ggatggttgt cagtccggat ttcctccggg cgattaatca gctggcagag ctgtcagata
      541 tcgtgttctc tcagggcgat gcgggggttc acttcgaact gatggcgcgt ccttcacggg
      601 atgttgccag agtgcagctt acccttgatg aacagaatct ggattacttc aatcagatgg
      661 aaagctggca gagcttcacc tggccgggga atacttacta tccaggtgtg tcgctcagct
      721 ggcggagtgt aagcagtggt atgcaactct acgccagtca ccaggggaac tgggggttta
      781 ttcgcctgct ggacgaagcc ctgatcacgc cacttgatgc cagccgcaca caactggtgt
      841 ggatcactgc cgatggtaat cctctgaaat tcgtcatgcg cagcgagctg ggcgacggtc
      901 cgcttgcctt actgaagcta caggggttta cgctgcccac cgccattttt aacgttgccg
      961 ccggcacgga aggtgttgag taaaaatgaa caggggcatt tatccgacgc cggcctttac
     1021 tgaagatctg ggggctgatg ggcacatttt tcaccctttc agagaacggc tccttcatgc
     1081 atgcccgtcg gcgttttcgc ccacatgtca gaaaaaacaa ccgataagct gacggaaggg
     1141 caaacccact cagcaggaga acgtatgctg ctgaaatgga ggccaccaca aatcttcaac
     1201 agtaaggacg acgcttctcc ggaagcggtc gtgaggttta tgaaagagac tgataaacag
     1261 gaaaaagaat gagttcaccg gaagccctgt taacggcctg cgccgccagc caggaagaaa
     1321 ggctgagcct catttcccgc gcaaatgagg ccctgtcgct ctgggataac tggctgcatc
     1381 catttacctc cggatctgaa accggggacg atcctgcata cgatgataat tttcagctga
     1441 tgcgtgaaga gatcaataag atttcaggta ctgattcagc gctgttgtgc gaactggctc
     1501 agaagtgcct ttgcgaatgc gccagagata ttcgtgtcgt gacctggtac gtgctggccc
     1561 gtctcacccg ggatggtgaa agagggttgt ccgaaggctt attacttctt gttgcgatgc
     1621 tgacccgcta cggacaggca tgtcatcccc ggcgtcctgc cgcccggaaa gctgcgctgg
     1681 agtggctgaa cagtgcaaaa gtgactgatg cactgctgtt atggcccggg gtggacagca
     1741 atgaggccgg tctgacagcg ggtgccataa gcctgctggg atccgccgtg gcagactggc
     1801 cggaagcgga gaagccgtct tttgcggggc tttgttccgc gcttgagaac cgcctggcac
     1861 gttcaggggg catggaggca ctggtccccc agaacagcag tccccgggag accgggcgtg
     1921 aatcagctcc cccggactca ccacaactgg cagcggtgac ctcggggcgc gacttactcg
     1981 accaggccag gttactttcc cgctggctga gcgggcagcc gcagggctgg ctggcttctc
     2041 accggctgat gaagacggtg cggtgggata cggtcgatca gctcccttcg ctggacagta
     2101 gcggacgcac ccgcctggtc cccccgaaaa ccgggtatcg cgctcagctt aagcgactct
     2161 ggcttcagaa aaactgggtt gagctgattg aactggccag tcagatgtat tgcgaaggtg
     2221 tgaatcattt ctggctcgat cttcagtggt atctctggca gggactcagt catgctggtc
     2281 agccctggga ttcctggtct gacgctgtcc tgtcagatct gcggctgttg cttaaacgtc
     2341 tcccgggcct tgaaggactg gcatggaatg acggtactcc ctttgctgac gaggtcaccc
     2401 tcgcctggat cgctgaaaaa gtgaatgaag aagatgtgat gtacggcgat gaagctgcca
     2461 ttcctgccgt tgttaatggc cagactgacg atgtgctgat ccttgagtct gaagccatgg
     2521 aaaagggaga cacggaaggg cctgaagcgg cgcttgcctg gctccagtcc cggccgggga
     2581 tggatacacc acgtcatcgc tggctgatac gcctgctcat ggcccgggtg gcagagcagt
     2641 atggccggaa cgatatggcg cttcatctgc ttggtgaact tattacccgt gcgccacagc
     2701 tgacgctgga agactgggag cccgctttgc tgtttgaagt ccgggcccga cgcctgaagc
     2761 tgctacggat gaaagccggt cgcagcgaat ctgataaagc ccgtctgatg cctgagatgg
     2821 atacgctgct ggcggggctg atcgccattg acccggcccg tgccatggtg ctctgtggct
     2881 gatgttctct ttaatcattt tcaggaatgc cgtgcatgga tgatttaacc ctgcgctatt
     2941 acgaagcaga gatgcgctat ctgcgggaag ccggtaaaga gtttgcccgt gctcatccgg
     3001 atcgggcagc aatgctgaat ctggataagc ctggcgcctg cgatcccggt gtggaacgtc
     3061 tgtttgaagg ctttgccttc ctgatgggac gtttgcgtga aaagctggat gatgacctgc
     3121 cggagctcac cgaagggctg gtgagtctgc tgtggccgca ctatatgcgg actatcccgt
     3181 cgctggccat cgttgagttc tcgcctgact ggcgtagtct gcgtcaggcc gaaatactgg
     3241 ctgaaggctt ctccgttttg tcccgtcctg tcgggccgca taaaaccgcc tgtcagta
//
LOCUS       DAZKGF010000307         3284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZKGF010000307 DAZKGF010000000
VERSION     DAZKGF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.307"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            251..769
                     /locus_tag="ACHOQV_004998"
     CDS             251..769
                     /locus_tag="ACHOQV_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP9633225.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            979..3087
                     /locus_tag="ACHOQV_004999"
     CDS             979..3087
                     /locus_tag="ACHOQV_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP9633226.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQVIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLMVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          833 a          815 c          936 g          700 t
ORIGIN      
        1 ttatggaggc aatttggact ttgaacagtt gggtatggtt atccagatat atattattaa
       61 cttattgctg aataagagat ttaatgattt taaaaatagt ttttacgaca accgtaaatt
      121 ctagctcggt ttgtatgtaa aaaatactga aaaatgatgc ggttccgtga agagagctgc
      181 atctgcctgt ctgggattaa cggcaggtca ttgtaagtag taattaacca cgttaacaag
      241 gagtttacaa atgccaacac cgtgttatat ctccatcact ggtcagaccc agggaaatat
      301 tactgcaggt gcatttaccg ctgattctgt cggcaacatc tatgtgcagg ggcacgaaga
      361 cgagatgctg gtgcaggaat tcctgcataa cgttactgtc ccgaccgatc ctcagtccgg
      421 ccagcctgcg ggtcagcgtg ctcacaagcc gttcattttt accgtggcgc tgaacaaagc
      481 tgttccgctg atgtacaacg cactggcctc cggcgaaatg ctgccaacca ctgaacttca
      541 ctggtggcgt acctctgtgg aaggcaaaca ggagcattat ttcaccaccc gcctgaccga
      601 ttcgacgatt gtcgatatga aactgcatat gccgcattgc caggatccag cgaaacgcga
      661 gttcacccag ttgctcgaag tttcactggc ctaccgcaaa atagagtggg aacacgtcaa
      721 gtctggcacc tccggtgccg acgactggcg cgcaccgctg gaagcataag ttaagccaac
      781 agcatccggc tatcaggccg gatgctgttt ttgtattacg ccgtaaaaag aaaggttgtc
      841 agtaagggat tacgttcacg ggagatggcg gatgccggaa acttcggcgg tagcgtgcct
      901 gaacgcaagc gtaacactgt actgttacag ccgctttaac agcgaactgt ctgtttttcc
      961 ttaaacccgg aggttgctat gtcaaccgga ttacgtttca cgctggaagt ggacggtctg
     1021 ccaccggacg cgtttgcggt ggtctccttt catctgaacc agtcactctc ttcgctcttt
     1081 tccctcgatc tctctctggt cagccagcag tttctctccc ttgaatttgc gcaggtgctg
     1141 gacaaaatgg cctacctgac ggtatggcag ggcgatgacg tacagcgccg ggtgaaaggt
     1201 gtggtgacct ggtttgaact gggggagaac gacaaaaacc agatgctgta cagcatgaag
     1261 gtgtgcccac cgctgtggcg cacagggctg cgccagaact tccgtatctt ccagaatgag
     1321 gacatcgaaa gcatactcgc gacgatcctg aaagaaaacg gggtgaccga gtggagtccg
     1381 ctgttcagcg agccgcatcc ttcccgtgag ttttgtgtcc agtacggcga aaccgattac
     1441 gatttcctgt gccggatggc ggcggaggaa ggcatcttct tttatgagga gcacgcgcaa
     1501 aaaagtaccg accagagcct ggtcctgtgc gacaccgtgc gttatctgcc ggagtccttt
     1561 gagatcccct ggaatccgaa cacccgtacc gaggtgagca ccctatgcat cagccagttc
     1621 cgctacagcg cacaaatccg cccatcttcc gtggtgacca aagactacac ctttaaacga
     1681 cccggctggg cagggcgttt tgatcaggaa ggccagcacc aggattacca gcgcacacag
     1741 tatgaagtgt atgactaccc cggacgtttc aagggtgccc acgggcagaa ctttgcccgc
     1801 tggcagatgg atggctggcg aaacaacgca gaagtggcgc gcggaacaag ccgttcgccg
     1861 gagatatggc cgggacggcg aattgcgctg acggggcatc cgcaggcgaa cctgaaccgg
     1921 gaatggcagg tggtggcaag tgaactgcac ggcgagcagc cgcaggcggt gccgggacgc
     1981 aggggttcag gtaccacgct gaataaccac tttgcggtaa taccggcaga cagaacatgg
     2041 cggccacagc cgttgctgaa accgctggtg gacggcccgc agagcgccgt cgtgacggga
     2101 ccggcaggag aagaaatctt ctgtgatgaa catggccgcg tgcgggtgaa atttaactgg
     2161 gaccgctata acccgtcaaa ccaggacagt tcatgctgga tccgtgtggc acaggcgtgg
     2221 gcaggcaccg gctttggcaa cctggcgata ccgcgtgtgg gtcaggaagt cattgtggac
     2281 ttcctcaacg gcgatccgga ccagccgatt attatggggc gtacctacca ccaggaaaac
     2341 cgcacccccg gcagcctgcc gggaacaaag acgcagatga ccattcgttc gaaaacctat
     2401 aagggcagcg ggtttaatga actgaagttt gacgatgcga cagggaaaga acaggtctac
     2461 atccacgcgc aaaagaacat gaacaccgag gtgctgaata accggaccac ggatgtgata
     2521 aacaaccatg ctgagaccat cggcaacaat caggtgattg cggttaccaa caatcagata
     2581 cagacggtgg gcgttaacca gatagagacg gtgggcagta accagatcat taaagtgggc
     2641 tctgttcagg tggaaacgat tggacttgtt cgtgcgctga ccgtgggcgt ggcgtatcag
     2701 acgacggtag gtggcattat gaacacctcg gtggccctga tgcagtcctc gcagatcggt
     2761 ttgcataaat cgttgatggt cgggctgggt tatgacgtca aagtcggaaa taacgttacc
     2821 ttcaccgttg gtaaaacgaa aaaggatgat accggacaga ccgcgattta ctccgccggt
     2881 gagcatctgg aactctgctg tggtaaggca aggctggtgc tgactaagga cggacaaatt
     2941 tttctcaacg gcacaaaaat tcatttgcag ggtaaggagc aggttaatgg tgactcactg
     3001 ttgattaact ggaactgtgc agcctcgaaa tccccaccga agacccctga tgaaaagcag
     3061 gatacgccgg atatgagaga gtactgatcc cgtacctgtt cggtattcag taacaaacaa
     3121 gcattaaaaa taaacagaag aaggaattga atcatgagcg gaaaaccagc ggcgcgtcag
     3181 ggagatatga ctcagtatgg cggtcccatt gtccagggtt cggcaggtgt aagaattggc
     3241 gcgcccaccg gcgtggcgtg ctcggtgtgt ccgggcggga tgac
//
LOCUS       DAZKGF010000308         3278 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZKGF010000308 DAZKGF010000000
VERSION     DAZKGF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.308"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..606
                     /locus_tag="ACHOQV_005000"
     CDS             <1..606
                     /locus_tag="ACHOQV_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9633227.1"
                     /translation="VTRLIYPAESKQITLSVQNSGDNTSYLIQSWVDDSKTEKKSQDF
                     VITPPLFMLPTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLA
                     LQNKIKLFYRPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPN
                     TFVPPKSDITVSSAVTLAGEITYQTINDFGATTERQKGIMQ"
     gene            643..3237
                     /locus_tag="ACHOQV_005001"
     CDS             643..3237
                     /locus_tag="ACHOQV_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFP9633228.1"
                     /translation="MNLKLKRCEYWMAAQKQMKRVVPLLLVIMPACSIAGMRFNPAFL
                     SGDTEAVADLSRFEKGMTYLPGSYEVEVWVNDSPLLSRTVTFKADDENQLIPCLSLAD
                     LLSLGINKNALPEQALALSENSCLDLRIWFPDVHYMPELDAQRLKLTFPQAIIKRDAR
                     GYIPPEQWDNGITAFLLNYDFSGNNDRGDYSSNNYYLNLRAGINIGAWRFRDYSTWSR
                     GSNSAGKLEHISSTLQRVIIPFRSELTLGDTWSSSDVFDSVSIRGIKLESDENMLPDS
                     QSGFAPTVRGIAKSRAQVTIKQNGYVIYQTYMPPGPFEISDLNPTSSAGDLEVTIKES
                     DNSETVYTVPYAAVPILQREGHSKYSTTVGQYRSNSYNQKSPYVFQGELIWGLPWDIT
                     AYGGAQFSEDYRALALGLGLNLGVFGATSFDVTQANSSLVDGSKHQGQSYRFLYSKSL
                     VQTGTAFHIIGYRYSTQGFYTLSDTTYQQMSGTVVDPKTLDDKDYVYNWNDFYNLRYS
                     KRGKFQASVSQPFGNYGSMYLSASQQTYWNTDKKDSLYQVGYNTSIKGIYLNVAWNYS
                     KSPGTNADKIVSLNVSLPISNWLSSTNDGRSSSNAMTATYGYSQDNHGQVNQYTGVSG
                     SLLEQHNLSYNIQHGFANQDNSSSGSVGVNYRGAYGSLNSAYSYDNEGNQQINYGISG
                     ALVVHENGLTLSQPLGETNVLIKAPGANNVDVQRGTGISTDWRGYAVVPYATEYRRNN
                     ISLDPMSMNMHTELDITSTEVIPGKGALVRAEFAAHIGIRGLFTVRYRNKSVPFGATA
                     SAQIKNSSQITGIVGDNGQLYLSGLPLEGVINIQWGDGVQQKCQANYKLPETELNNPV
                     SYATLECR"
BASE COUNT          963 a          625 c          728 g          962 t
ORIGIN      
        1 gtaacgcgat tgatttatcc ggcagaaagt aagcaaataa cactcagtgt gcaaaatagc
       61 ggtgacaaca ccagttattt aattcaatca tgggttgatg acagtaaaac ggaaaaaaag
      121 agccaggatt ttgttatcac tcctcccctc tttatgttgc caacgcgtaa agaagcttca
      181 ttacgtatca tgtttcttgg taaaactaat ttgccgaccg atcgtgaaac tttgtattgg
      241 atgaatgtta aagcaattcc gccaacagat gagaaaaata cacagaagaa taccttacag
      301 ttggcattac aaaacaaaat caagctattt tatcgcccgg aaaatctacc tgtgcaacca
      361 ggaaaagcac gaaatatgtt gcggtttaag tatgagggca aacaactaaa agttatcaat
      421 ccttcgcctt attacctgac actgacagga attaaagtac aagggagtaa attgcccaat
      481 acgtttgtac caccgaaaag cgatattacg gtctcgtcgg cagtgacgtt ggctggtgaa
      541 attacttatc aaacaattaa tgactttggc gctacgacgg agcggcagaa aggaataatg
      601 cagtaacaac gggatagttc agatgcaaca caggtcaggg atatgaacct aaagctcaaa
      661 agatgcgaat attggatggc ggcacaaaag cagatgaaac gggttgtacc gcttcttctg
      721 gttattatgc ctgcatgttc aatcgcggga atgcgcttta accctgcttt tctgtcgggt
      781 gatactgaag ctgttgctga cttatcccgt ttcgagaaag ggatgactta tcttcctggt
      841 agctatgaag tcgaagtttg ggtcaatgat tcccctttac tctctcgtac tgtaactttt
      901 aaagcagacg atgagaatca actgattccc tgcctttcac ttgctgactt attaagcctt
      961 ggaattaaca agaatgcgct gccagagcag gctttggctt tatctgaaaa tagttgcctt
     1021 gatttgcgta tctggtttcc tgatgtgcat tacatgccgg agctggatgc acagagactt
     1081 aaactgacct ttccgcaggc gataataaaa cgtgacgccc gcggatatat tccaccagaa
     1141 cagtgggata acggtattac agcttttttg ctgaattatg acttttctgg taataacgat
     1201 cgtggtgatt actcttcaaa taactattat ttaaatcttc gcgctgggat caatattggt
     1261 gcatggcgtt ttcgcgatta ttcaacctgg agtcgtggga gtaattcagc aggtaaactg
     1321 gagcatatca gtagtacgtt gcagcgcgtt attattcctt tcagaagtga attaacgcta
     1381 ggagatacat ggtcatcatc agatgttttc gacagtgtta gtattcgtgg cataaaactg
     1441 gaatctgacg aaaatatgtt gcccgatagt caaagtggtt tcgctcccac ggtgcgcgga
     1501 attgcgaaaa gtcgcgctca ggtaacaatc aaacagaatg gttatgtcat ttatcaaacc
     1561 tatatgccgc cgggaccgtt tgagattagc gatcttaacc cgacatcatc tgcgggagat
     1621 ctggaagtta ccatcaaaga gtctgataat tcagaaactg tctataccgt accttatgcc
     1681 gctgtcccca tcctgcaacg agaaggtcat tcaaaatatt ctactacggt tggccaatat
     1741 cgaagcaata gctataacca gaaaagtcct tatgtatttc agggggaatt aatttggggt
     1801 ttaccctggg atattacggc ttatggtggg gcacaattct ctgaggatta ccgggcgttg
     1861 gcgctcggcc ttggcctgaa tctgggtgta tttggtgcaa catcgtttga tgttactcag
     1921 gctaacagtt cgcttgtgga tgggagcaaa catcaagggc aatcttatcg ttttctttat
     1981 tccaaatcgt tagttcagac aggaacagca ttccatatta ttggctatcg ttattcaacc
     2041 cagggctttt acactttaag tgatacgaca taccaacaaa tgtcagggac tgttgttgat
     2101 ccaaaaacgt tagatgataa agattacgtt tataactgga atgattttta taacttgcgt
     2161 tatagcaaac gtggaaaatt tcaggctagt gtatcgcaac ctttcggtaa ctacgggtct
     2221 atgtatttat cggctagtca gcaaacatac tggaatactg ataaaaaaga ttctttatac
     2281 caagttggtt ataacaccag tattaagggt atctatctaa atgttgcgtg gaattacagt
     2341 aaatcaccag ggacaaatgc ggataaaatt gtctcgctaa atgtctcatt acctataagt
     2401 aattggttat cttccacgaa tgatgggcgc tcatcatcga atgccatgac tgcaacgtat
     2461 ggttatagtc aggataacca cggacaggta aaccaatata cgggggtatc tggttctctg
     2521 ttggagcagc ataatctcag ttataacata caacatggtt ttgctaatca ggataatagc
     2581 agtagtggtt ctgttggtgt taattatcgt ggggcatatg gttccttgaa ttccgcctac
     2641 agttacgata atgaaggtaa tcaacagata aactatggca tcagtggtgc tcttgttgta
     2701 catgaaaatg gtcttacgtt gagtcaacca ttaggtgaaa ctaatgtttt gataaaagcg
     2761 cctggagcga ataatgtgga tgtccagcgg gggacaggaa tatccactga ctggcgtgga
     2821 tatgcagttg ttccttatgc aacagaatat agacgtaata atatttcatt agatcctatg
     2881 tcaatgaata tgcatactga actggatatc acttccactg aagttattcc gggaaaaggt
     2941 gcgttagttc gtgcagagtt tgctgctcat atcggtattc gtggtttgtt cacagttcgt
     3001 tatcgtaata aatcagtccc attcggtgct acagccagcg cacagattaa aaatagtagt
     3061 caaattaccg ggattgtcgg cgataatgga caactttatc tctcaggatt gcctttagaa
     3121 ggtgttatta atatccagtg gggagatggt gttcagcaaa aatgtcaggc taattacaag
     3181 ctccctgaaa cagaactgaa taatcctgtt agctatgcta ctctggagtg ccgctaatga
     3241 atttaatatt ctctaaatat tctgtatttt ttattctt
//
LOCUS       DAZKGF010000309         3264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZKGF010000309 DAZKGF010000000
VERSION     DAZKGF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.309"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(11..321)
                     /locus_tag="ACHOQV_005002"
                     /pseudo
     CDS             complement(11..321)
                     /locus_tag="ACHOQV_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(717..1526)
                     /gene="iolB"
                     /locus_tag="ACHOQV_005003"
     CDS             complement(717..1526)
                     /gene="iolB"
                     /locus_tag="ACHOQV_005003"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFP9633229.1"
                     /translation="MTNLLSTCTTRAGNIQHISPQNAGWEYVGFDVWLLKAGESLTLP
                     SDDRERCLVLVAGLASVKAADHFFYRIGQRMSPFERIPAYSVYLPHHTEAKVTAETDL
                     ELAVCSAPGFGELPVRLISPQDIGVEHRGKGRNQRLVHNILPDTQPADSLLVVEVYTR
                     EGDTSSWPAHKHDTAVEGQETYLEETYYHRFDPPQGFSLQRVYTADRSLDECMAVYNR
                     DVVKVPKGYHPVATIAGYDNYYLNVMAGPLRKWQFTWEADHAWINSSEYPR"
     gene            complement(1551..3056)
                     /gene="iolA"
                     /locus_tag="ACHOQV_005004"
     CDS             complement(1551..3056)
                     /gene="iolA"
                     /locus_tag="ACHOQV_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate-semialdehyde dehydrogenase IolA"
                     /protein_id="HFP9633230.1"
                     /translation="METVGNFIQGKTRLSNSGETLPVTNPATGEVIRLVTQSTRQDML
                     EAIQSAHEAFPAWSKMAPLRRARILFEFKVLLEKHRDELAALIVSEHGKVWSDALGEL
                     TRGIEVVEFACGIPHLIKGEFSFNVGTGVDSFSLMQPLGVVAGITPFNFPAMVPMWMF
                     PIALACGNTFVLKPPALVPSASLRMAQLLQEAGLPDGVFNVVHCGNDAASLLTSDPRI
                     QAVSFVGSSSVAEHIYTTASAHGKRVQAFGAAKNHAIVMPDADLDATVNAIMGGAFGS
                     AGERCMALPVVVAVGDETANRLIERLKPLIAALRIGPGELREKDENEMGPVVSRMHQQ
                     KVLAYIDKGVSEGATLVMDGRNYCVKGYPEGFYVGGTLFDNVTPEMTIWREEIFGPVL
                     GIVRVTDYATAIRTVNSHEFGNGSVIFTSNGHYAREFAQSVEAGMVGINVPVPVPMAF
                     HSFGGWKRSVFGALNVHGPDGVRFYTRMKTVTARWPNGQQIASEFSMPTLG"
BASE COUNT          863 a          800 c          752 g          849 t
ORIGIN      
        1 aaccattcgg ctatattatc tttttcatgt tggtgctttt attctgtgag gtgtgctggc
       61 atggtggaca caaccatttg ttctttttag ctgccatttg ataatatttt cctgaagctt
      121 tccggggata tattccccag acgggagtgg cggcgctgac ggttgtagaa tatctcaatg
      181 tatacccagt attactgaga tgacttcatt ccggctacta aaatggtagt ggttcagact
      241 ttcattcttc agagttcccc aaaagctttc cataggggtt ttgtcgtaac agttcccttt
      301 acgagacatt gatgttttca gagctaactg cgatagactc cttcaacttc aacttcaact
      361 tcaacttcaa cttcaacttc aacttcaact tcaacttcaa ctgagaaacg gatgatttca
      421 cgatagactg cccaacgtat tgagcccgtt aggaagccag agtaacaagc cgccgtgtac
      481 ggcatttctc tcctaattcg gcagaaccaa aaatattgtc agctcaggag taagaatcag
      541 aaaaaaccat aacagaacac cagaaacagg atattatcag acagagaaaa aattcaaaaa
      601 aatccaccga ttctgttgcc tgtggatttg tgtataagag gcagaaccct gttggggact
      661 gtgcacaaaa aagccagctg cagaggacaa ctggcaaagg aatatttaac gtattgttaa
      721 cgcggatact ctgacgaatt aatccatgcg tgatcagctt cccatgtgaa ttgccatttg
      781 cgtaatggcc cggccatcac attcagataa tagttatcgt aacctgcgat agtggccacc
      841 ggatgatagc ctttcgggac ttttaccaca tcgcggttat atactgccat acattcgtcc
      901 agactgcgat cggcggtgta gactcgctgt aagctgaatc cctggggtgg atcgaaacgg
      961 tggtagtagg tttcttccag ataggtttcc tgcccttcaa ctgctgtgtc atgcttatgg
     1021 gcaggccagg aacttgtatc gccttccctt gtatatactt ccactaccag cagactgtca
     1081 gcaggttgag tgtctgggag gatattatga accaagcgct gattgcgccc ttttccccta
     1141 tgttccaccc caatatcttg tggtgagatc agtcttaccg gaagttcacc gaatccagga
     1201 gcactgcaga cagccagttc cagatcagtt tcggcagtga cttttgcttc tgtatggtgc
     1261 gggaggtaaa cagaataagc cggaattcgc tcaaaaggac tcatgcgctg gccaatccga
     1321 taaaaaaagt ggtcagcggc ctttacgctg gcaagtccgg caaccagaac caggcaacgt
     1381 tcgcgatcat cggatggcaa ggttaaagat tcgccggctt tcaacagcca gacatcaaaa
     1441 ccgacatact cccagcctgc gttttgcgga gaaatatgtt gaatatttcc agccctggtg
     1501 gtgcaggtac tcagcaagtt ggtcataatc ttctccttcc tgaacgttta ttaacctagc
     1561 gtaggcatac tgaactcgga tgcgatctgc tgaccgttcg gccagcgtgc ggtaacggtt
     1621 ttcatccggg tataaaaacg caccccatcc ggtccgtgga cattcagcgc cccaaacacc
     1681 gagcgtttcc agccgccgaa agagtgaaac gccattggaa ccggtaccgg aacattgata
     1741 ccgaccatcc cggcttcaac cgattgagca aattcgcggg cataatggcc gttggaggtg
     1801 aagatcacgc taccattgcc aaattcatga gagtttaccg tacgaatggc ggtggcataa
     1861 tctgttacgc gtacgatgcc gagcacggga ccaaaaattt cctcacgcca gatagtcatt
     1921 tccggcgtga cgttatcaaa caaggtaccg ccgacataaa atccttcagg atagcccttc
     1981 acgcaataat ttcgcccatc catcaccagt gttgctcctt cgctaacgcc tttgtctata
     2041 tatgcaagga ctttttgttg atgcatacgt gaaacaactg gccccatttc attttcgtct
     2101 ttttctcgta actcaccggg gccaatacgc agcgctgcaa taagtggttt taggcgttca
     2161 ataagacggt tggctgtttc atcaccgaca gcgaccacca caggtaatgc catacaacgt
     2221 tcgcctgcgg aaccaaaagc cccacccata atagcgttaa cggtagcatc aaggtcagca
     2281 tccggcatga caatagcgtg gttttttgcg gcaccgaaag cttggactcg cttaccgtgt
     2341 gcactggcgg tggtgtagat atgctcagcg acggaagaag atccgacaaa actgacggcc
     2401 tgaatgcgtg gatcactggt caacaggctg gctgcgtcat tgccacagtg aactacattg
     2461 aatacaccat ccggtagtcc ggcctcctgt agtagttgcg ccatacgtaa cgatgcggag
     2521 ggcaccagcg ctggaggctt caggacgaag gtattgccgc aggcgagagc gatagggaac
     2581 atccacattg gcaccattgc agggaagtta aagggtgtga tgccagctac aacaccaagt
     2641 ggttgcatta atgaaaagct gtcaacgcct gtgccaacat taaaagagaa ctctccttta
     2701 atgagatgcg gaatgccgca ggcgaattca acaacttcga ttccgcgagt cagttcacca
     2761 agcgcgtcgg accatacttt gccatgttca ctgacgatca gagccgccag ttcatctcgg
     2821 tgtttttcca gcaacacttt aaattcaaaa aggattcttg cacggcgcaa cggtgccatt
     2881 ttactccatg caggaaaagc ttcatgagca ctctgaatcg cctccagcat atcttgccgg
     2941 gtgctctggg taaccaggcg aatcacttct ccggttgcag ggttagtcac cggtagtgtt
     3001 tcaccactgt tgctcagtct cgttttaccc tgaataaaat ttccaactgt ttccatcact
     3061 gtttaccttc ttgaatcaat gttaacgacg ttccgactga gtgttcacag gcaaagcctg
     3121 atcatctaca gtctaaaatc acacagtgtt taatgtatag cttcggatta taaatttcaa
     3181 atttaaattt atgaaaaaat aagttcactg catggggggt gatggttata ggttgcttgt
     3241 tgttaactat ttgaatatca aaga
//
LOCUS       DAZKGF010000310         3229 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZKGF010000310 DAZKGF010000000
VERSION     DAZKGF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.311"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(72..3020)
                     /gene="ehaB"
                     /locus_tag="ACHOQV_005005"
     CDS             complement(72..3020)
                     /gene="ehaB"
                     /locus_tag="ACHOQV_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFP9633231.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWADEGTTGWFGNTGNASDYNGDGWNADDVALAVIAHPYAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPQGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
BASE COUNT          866 a          741 c          747 g          875 t
ORIGIN      
        1 cagtaatgcc cgtcaaatcc ttgacgggca ttatttagat taaattacct atttcttcgg
       61 agtgaagaat attaccaggt atatttaaca cccacattcg cggaccagtc ttgatctacg
      121 tcaccaccac cgaggtagtt agcatcggta taggcgctga agttcttggt gaagctgaac
      181 tgagtaccca gaccaacacg taccgcagac ccttcagtac cgttatcgat ggaatcaccg
      241 ttcacatcgt tatcgttgtt agagtcgtcg tagacgtaag ccagtttgaa gtacggagtc
      301 agagcctggt cttcgctgta ggtgaaggta taacctgcat ctacacccag ttcataacgc
      361 atgctgtcgt aagactgacc atcaactttc atgtcgttgc tcagctggta atcatcacca
      421 gactggaaca gaccagaaac gctgccgtaa ggagtcacgt aacctgcatc acccagtttg
      481 aagtcgtaac cggctttcaa accaaagccc caagcgtcgg agttggtgct gccgtcaacg
      541 taagtaccgt tgctcatggt tgcagacagg tcgttgttga agtgagagta gctcaagcta
      601 ccgtcaacaa agacgttgtt cgcgaagtga gcagaagagt agatgtaggc agtctggctg
      661 tcttgatcca cctgaccaga acggtcattc atatcacctt tagcgaagcc tgcagccgca
      721 ccgacgatcc acttagcgtt gttaccgtca attttggtat caacaccgac catgatgccg
      781 ttaacatcct gatcatagtt gatggtgccg ttgtcgccgt tgaagttacc accgaagtag
      841 cttacccatg cgccgccgtt atcagccaga ccatgacgag agttggtcag acgagtacca
      901 acggtgtctt gttccaggtt ccagatattg gtgttcgcag acgggatgct cagcgccatg
      961 ttagcgtagt cggtcagctc catctgttgc agaacaacgg tgttaccacg ctgctcagcc
     1021 tgataggtgt atgcacccag gtcagctttg ttagcagcag agaaggtcgc gttgctgttt
     1081 ttgtcgttga cgtagatcag ctcgttgccg ttgtagtcag cgatagaacc agcaccagta
     1141 gcgttgtcga tacgaacttt gtagttaccg gttgcagctg caacatggtc accgttgtta
     1201 tcaacttcgt tctgaccagc ttcgatagaa gccgtgttgt cgttatcgcc gttaccgtta
     1261 atggtcaggt gaccatcaga gttcattgcg attaagccat aaccgtagtt tgacttggta
     1321 gtatcgttgg tacggtcgtt ggtcagatcg gcatcgagta cgaagcggtt gctttcgatg
     1381 ttgaacacac ctgcgtgaat gttaccactg gtatcagtag tgctggtcag gttcagagta
     1441 tcaacctgca gagcattcgc cgcagtgtta cctacatcca ggataccgtc gttagtcact
     1501 ttgatgttgt tagcatacag agcagcatca cctacagccc agccagcaga aacttcatca
     1561 gaaatggtta cggtactgtt gtcgacagtc agtgaatcag ttgcaacatg accatcttcg
     1621 ttgatgttca gcgcagaaag accagtcaga gagatagagt cagaaatcag cgttgaatca
     1681 gcaacattaa cagttgaacc actgttgatg ctcagagtat caatcagaga agttttggtg
     1741 gtatcccact gtgaaccggt gttcagggtc acattgaaca gaccgctctg gtaaacttcg
     1801 ttaccgatga tgtgatcaaa ttcgctgtat tcagagtcgc tgttttctac accaacagtt
     1861 gatgaaggcc acaggctgtt agctgcgatg tcgatcagag ttgcagtggc ttcagtattg
     1921 attccatatg tataggagtc atttacacca tcgtcatcag tatcaacacc aatctgatgc
     1981 gcagacattg ccgcaccgac ccacttgctg ccattgttca gggtcagatc caggcgatca
     2041 gtaccatccc aaccgttagt atctacatca ccgtcagcat cacgatagct atcacgacct
     2101 tgtgggaaga agttctcatc aaaattactt gagaagaata catcgcccat cagtgtggaa
     2161 ttgtcgaatg ttgcagtggt ttgcatggcg ttatcagcat atggatgtgc aataacagct
     2221 aatgcaacat catcagcatt ccacccatca ccattgtaat cactagcatt accagtatta
     2281 ccaaaccaac cagtagtacc ttcatcagcc catgaaccag aggtcacggt agaatcttta
     2341 acagtgatgg tgttattaaa tacttcgctg ctgtttacac cagtgctgac agtgttgtcg
     2401 tcgatatctt cccactcata accctgagtc agcgtaatac ctgctacatg agagttatta
     2461 gagatggaca gatcgacttc ctgatccaga gtgatggcag taccaatgtt aaatacattt
     2521 acaacatgag tgtcagcggc attattgtaa gtgccgttat atgtgtagtg ttcgtagttg
     2581 tcatcgatcg ttgaattatc aacggtcagc gccagacgat catggtagta gtcatccgca
     2641 cgatcagcac aatcagttgt catgcactcg gaataaatca taccgtgaat agtgctgttt
     2701 ttgattgtca ggctattggc gttggtatta gtagaaatac catcgtccag atagtaggta
     2761 gaaatcacgc cgttaacggt agcgttatta atgaccggat agatatcgcc gttgtaataa
     2821 ctatcagcgg catagttatt ccagcctaca taaccatcat agtaaacgtc atcagcatag
     2881 gtggcgtcgt tgtagtgatg gaaagtattg taagtttgac cagaaatatc gttggtcgct
     2941 gcattagcct gagaggtgat agccagagtg caagccaatg ctaattgtga tactacaagt
     3001 ttctttttcc aggagtgcat ttgtcatccc tcctcaggga cgtaataagt tgatccatca
     3061 attatcaatg catagaaaac atattatgtt tcgcgtttac gattatgcgg cgttatagag
     3121 gaatggttca attattcttt cccctaagtc cgattgcgga catatataaa gcacgtgaac
     3181 acacacaaag aaagaaataa caaaaacaca atatttcaat acgttaacg
//
LOCUS       DAZKGF010000311         3163 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZKGF010000311 DAZKGF010000000
VERSION     DAZKGF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.312"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1020)
                     /locus_tag="ACHOQV_005006"
     CDS             complement(<1..1020)
                     /locus_tag="ACHOQV_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9633232.1"
                     /translation="MGKLTLVEQALRLHKEYYGDECFGTIVKNYDYYPKVAEIFINRK
                     NKNTYSNLDIEPVASSGVVHTGSNPVMVKVGRTKKLMFKDGSGAFRIHLGKNEVVHIV
                     RYIHNSSIRMEYAIGTTKGLFMLHNLCEAGRVKLNRSLPRKGIWKIGDYQGIYYYGKS
                     RKEEIENALRFSGHPKFSELENDFNQFFSDIDYYTRYGQSGMRKILFTGPPGTGKTTI
                     AKALGAKYQDKYVFVYADNYFREVCYAAANKKIPLIIIAEEADELYRADAGTLSFLDG
                     ADTPRNPAGTYVIFSTNYPRRIDPRIRKRPGRIDRVISIGAFRTKDAASCAKMYLPEG
                     ININIK"
     gene            complement(1038..1550)
                     /locus_tag="ACHOQV_005007"
     CDS             complement(1038..1550)
                     /locus_tag="ACHOQV_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFP9633233.1"
                     /translation="MKITLTIFIFLLTTFQAANAGGIPGALAGILSTLNQEIQGKVIR
                     VLDGDTIEVLQDKKPVRIRLANIDAPEKKQAFGRWSANQLKALLAAQPVTVTYTQTDR
                     YGRIIGRVFTTNGTDASRFMVQSGAAWVYERYNTDKSLPALQREAQEQKRGLWADTNP
                     VPPWEWRKNN"
     gene            complement(1629..2204)
                     /locus_tag="ACHOQV_005008"
     CDS             complement(1629..2204)
                     /locus_tag="ACHOQV_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9633234.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKEYPVKELPDIDESAHPF
                     YVCYGGYLLNPGVSDSDISRKIKDQTGFWLAAIDDTHMDCHSIAYYDIHTLPLISCGR
                     QKIVPFAALIKADRCIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCEHR
                     FRQPVGEDNWKQAISEECAIRYANRLIQCEG"
     gene            complement(2321..2527)
                     /locus_tag="ACHOQV_005009"
     CDS             complement(2321..2527)
                     /locus_tag="ACHOQV_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633235.1"
                     /translation="MSEIRFVTLSSDTDINKIIQEGLTQKDATTTIVLGCASEGKEAP
                     LRLIETCFRTIPKHISIEEINEIK"
     gene            complement(2540..2929)
                     /locus_tag="ACHOQV_005010"
     CDS             complement(2540..2929)
                     /locus_tag="ACHOQV_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633236.1"
                     /translation="MDKSDEEKIGYILDEHERIFRKQASLYTVLGILNLIICLLCVVA
                     TFCVSVILPESHLKYQLFSFTILCMVCSFIIYYYTRHMVLNRQELLRLMRLTEGAPDV
                     KQELLNRLLSGKILTYWDEKDIRRLIF"
BASE COUNT          946 a          698 c          522 g          997 t
ORIGIN      
        1 tttaatattg atgtttatac cttctggtaa atacatcttt gcacaactag cagcatcttt
       61 agtcctgaaa gcaccaatag aaataactct gtcaattctt cccggacgtt tcctgatacg
      121 aggatcaatt ctcctgggat agtttgttga gaatattaca tatgtcccgg caggatttct
      181 cggcgtatca gccccatcca gaaaacttag cgtaccagca tcagctctat aaagctcatc
      241 agcctcttca gctataataa taagaggtat tttcttgttc gctgcggcat aacaaacttc
      301 tctaaaatag ttatcagcat atacaaatac atacttatcc tgatactttg ccccaagagc
      361 tttggctatt gtcgttttac ctgttcctgg tgggcctgta aacagaatct tcctcattcc
      421 agactgacca taacgcgtat agtaatcaat atcagaaaaa aactgattaa agtcgttctc
      481 cagttcagaa aatttaggat gacctgaaaa acgtaaggca ttttcaatct cttcttttct
      541 agatttaccg taataataaa tcccctgata atcacctatt ttccagatac cttttcttgg
      601 taacgaacga tttagtttaa cgcgccccgc ttcacaaaga ttatggagca taaacaaacc
      661 tttagttgtt cctatagcat attccattct aatactggaa ttatgaatat atctgacaat
      721 atgcacgact tcatttttcc ccaaatgaat tctgaatgct cctgacccgt ctttaaacat
      781 gagtttttta gtccttccca ctttaaccat tactggatta cttcctgtat gaacaactcc
      841 agaagaagct acaggttcga tatcaagatt tgaatatgta tttttatttt tcctgtttat
      901 aaatatttct gctactttag gataataatc ataatttttt acaatagtgc caaaacattc
      961 atcaccataa tattctttat gaagccgcaa agcttgctct accaaagtta atttccccat
     1021 atgttacctt taataaatca gttgtttttc cgccattccc acggtggaac cggatttgta
     1081 tcagcccata aaccacgctt ttgttcctgc gcttcccgtt gcaaagccgg taatgattta
     1141 tctgtgttgt aacgttcata tacccatgca gcgccagact gaaccataaa gcggctggca
     1201 tccgtgccgt tcgtcgtgaa cacacgaccg ataatgcgtc cgtagcggtc tgtctgcgtg
     1261 tacgtcacag taacaggctg tgctgcaagc agagctttaa gctgatttgc tgaccagcgc
     1321 ccgaaagcct gttttttctc cggtgcatcg atgtttgcca ggcgaatcct gacgggcttt
     1381 ttgtcctgta aaacttcgat ggtatcgcca tcgagaacac gaataacttt cccctggatt
     1441 tcctggttta atgtagaaag tattcctgct aaagcaccag gtattccacc tgcatttgca
     1501 gcctgaaagg tagtaagcag aaaaataaat attgttaatg taattttcac aaaatactaa
     1561 ccccctccgg ttgttcaact ccgcaaatcc cagattaaaa tcaggaatct gcggagttta
     1621 tgatttgctt atccttcaca ttgaattaat ctgtttgcat agcgaatagc acattcttct
     1681 gatattgcct gtttccagtt atcttcacct acaggttgtc tgaacctgtg ttcacagcca
     1741 ttaaaagcaa aaatcccctc gcgattaagt atgttggttg caatatccca ctctttaatc
     1801 cttaaaaatg ccgttactgc aaaaccagaa tatgatgcaa ttttcgaaat gatgcatctg
     1861 tcagctttta ttaacgcggc aaaaggaact atcttttgac gtccacacga aatcagaggg
     1921 agtgtgtgaa tatcataata agctattgaa tggcaatcca tgtgagtatc atcaatagct
     1981 gccagccaga atcctgtctg gtcctttatt tttctgctaa tatcagaatc tgaaacacca
     2041 ggatttaata aatatccacc ataacagaca tagaatgggt gagcagattc atcaatatca
     2101 ggaagctctt ttaccggata ttctttccag tccttcagaa caccgaactc aagagcatcc
     2161 agcaaaaact ttcgttcaaa tccatcaaac aatgcatatt gcatatacta cccctcacag
     2221 aatgcctgat ttatataact aatgcgctat caatagatgg ctgtctctta aatataatga
     2281 gattcgataa taccagaata acaacgacta ccgtcatcat tcattttatt tcattgattt
     2341 cttcaattga aatatgcttt ggtatagtcc tgaaacaagt ctctatgagc ctgagcggtg
     2401 cttctttacc ctcactcgcg caacccaaca caattgtagt tgtagcatct ttttgagtaa
     2461 gaccttcctg aataatcttg ttaatgtcag tgtcagatga cagagttaca aatctgattt
     2521 cgctcatatg attacctctt caaaaaatta gacgtcggat atctttttca tcccaataag
     2581 tcagtatttt cccggatagc agacggttaa gcagttcctg tttcacatct ggtgcgccct
     2641 ctgtcagacg cataagtcga agcagttcct gacggttcag gaccatatgg cgggtgtaat
     2701 aataaataat aaaactgcat accatacaaa ggatagtaaa ggaaaataac tggtatttca
     2761 gatgtgattc cggcaatata acagaaacac agaacgtggc aacaacacaa agaagacata
     2821 taattaaatt gagtataccc agtaccgtat ataaagaagc ctgttttctg aatatccgct
     2881 catgttcatc cagaatataa cctatttttt cttcatctga tttatccata tatttcattc
     2941 ccatagttga tgtggaaata gttattcctc tccgcaaatc taggataaac atatgtcatc
     3001 tgcggagaat agttcaaacg cctctagttt agttctaaag cctttgatta ttttattcaa
     3061 aaaagttttc ttccaaactt actcataatt ttactgacgt gagcaggatc tgccccaggg
     3121 caattcatta aaaatgcttt tctcgcatca aaagttttac tcc
//
LOCUS       DAZKGF010000312         3157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZKGF010000312 DAZKGF010000000
VERSION     DAZKGF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.313"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2272
                     /locus_tag="ACHOQV_005011"
     CDS             <1..2272
                     /locus_tag="ACHOQV_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HFP9633237.1"
                     /translation="YILFFKKKKTKSAGSYTSAPVKAPPKEWLANNKKNQSVMQNEDS
                     EDDNLATFTLSGGRNVEYRITATQRQNFSKTTGALARWIFPGEMITVSGIEVTGGHFY
                     LGQRMKPAGHNSGGYYDGNNEASLVDDTLKIYPTPYIYEDSSLGYWPSFSSLSPEARG
                     AYLSWLASDRCDTSCPIGYVFIYLYGLERRALVDSRDNNISDDEFYSLFKEICRLRSV
                     FNENRSFRNYSSQLLEAMSILRPNENLAAALNDDSEFSNSMQFRLILAKTVDTGAPVP
                     ADLALTWVTNHAEYSLRTPARRCAKEFAALFKRRYTLKYGEGMVVKPNKARLRLDYTP
                     ASPSLRGIRLPVPDLPDPGALKGPVQKLMAIADICTGELDAYSRYLGRKGTSANDTAA
                     ILLLPSEIVNESAEKILSTFKHWADDAIRSKGGIVSVADYWSHMNATCPAKINKKEAD
                     LMQAFAQKMGYCLAPDPYHHHVKADVDGVLVLFPAGERGRFSPYPEFITAVLTLRLGS
                     VVALIDNSLDQAEQKVLENAINNNASFSDDEKRSLHAYLTWQLHTPANMTGMKSRIEL
                     LGAAEKSAVGKVIVSVACSDGRITPAEIKQLEKIYTSLGLDPSSVSSNIHQHSATETA
                     LVSSVPANQSTAGFTLDANVLARHESATDDVRKLLNSIFTEEEPEQPESAPASLTGEG
                     GLDSAHSQLYRRLLEKEQWSRKEATELCSNWNLMLGGALEVINDWSYAVVDAPVLEDA
                     DDHIWIDLEIAKELEG"
     gene            2276..2677
                     /locus_tag="ACHOQV_005012"
                     /pseudo
     CDS             2276..2677
                     /locus_tag="ACHOQV_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
     gene            complement(2648..2856)
                     /locus_tag="ACHOQV_005013"
                     /pseudo
     CDS             complement(2648..2856)
                     /locus_tag="ACHOQV_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2891..3025)
                     /locus_tag="ACHOQV_005014"
     CDS             complement(2891..3025)
                     /locus_tag="ACHOQV_005014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633238.1"
                     /translation="MSEIKIIGIELAKTNFYPFNINDYGKTVGKIKFSRSNLLNLLVQ
                     "
BASE COUNT          884 a          708 c          730 g          835 t
ORIGIN      
        1 ttatattctt tttttcaaaa agaagaaaac taagtccgcg ggctcttata cttccgctcc
       61 tgtgaaagca ccacctaaag aatggcttgc taacaacaaa aaaaatcagt ctgtaatgca
      121 aaacgaggac agcgaagatg acaatctggc tacgtttaca ctaagtggcg ggcggaatgt
      181 cgaatacagg ataacagcca ctcaacgcca gaatttctca aaaactactg gtgctttggc
      241 tcgttggatt tttccgggag agatgatcac agtcagtggg atagaggtta ccggcggtca
      301 cttctatctt gggcagcgta tgaaacctgc cgggcataac tcaggcggtt attatgatgg
      361 caataacgag gcatcgctag ttgacgacac gcttaaaata taccccacac cttacattta
      421 cgaagatagt tcattagggt attggcccag tttttcctca ctttcacctg aagctcgtgg
      481 tgcgtacctg agttggcttg ccagcgacag atgcgacact tcctgcccaa ttggttacgt
      541 tttcatttat ctgtatggtc tggaaagaag ggcgcttgtt gacagcaggg acaacaatat
      601 atccgacgat gagttctatt ctcttttcaa agagatctgc cgtctcagat cggttttcaa
      661 tgaaaaccga tctttccgga attactcatc ccagttactt gaagcgatgt ccattcttcg
      721 tccgaacgaa aatcttgcag cagctctcaa cgatgattcg gagttcagca atagcatgca
      781 gttcaggtta atcctggcaa agactgttga tacaggagcc ccggttccag cggatctggc
      841 tctgacgtgg gtgacaaacc atgctgaata ctcattacga acccccgccc gccgctgtgc
      901 gaaagaattt gctgcactat ttaaacggcg ttacaccctt aaatacggtg aagggatggt
      961 agtgaagccg aataaagcgc gtctgcgttt agattacacg cctgcaagtc ccagtttgcg
     1021 aggtattcgg cttcctgttc ccgacttgcc tgatccaggt gcattgaaag gcccagttca
     1081 gaagcttatg gccattgccg atatatgcac aggtgaactg gatgcttaca gtcgttacct
     1141 tggtagaaaa ggtacttcag caaatgatac cgctgcaatt ttgttgctgc cttcagaaat
     1201 cgttaacgaa agcgcagaaa aaatattaag tacattcaaa cactgggctg atgacgcaat
     1261 ccgcagtaag ggtgggatag tttcagttgc tgactactgg tcacatatga atgccacctg
     1321 tccggcgaag atcaataaaa aagaggccga cttaatgcaa gcctttgcac agaaaatggg
     1381 atactgtctg gctcctgacc cctatcacca ccacgtaaag gcagatgtcg atggtgtact
     1441 tgtcttattc cctgctggtg aaagaggacg tttctcacct tatcctgaat ttatcacagc
     1501 tgtattgacg ctccgattag gttcggtagt cgccttgatt gacaattctc tcgatcaagc
     1561 cgaacagaaa gtgcttgaga atgccatcaa caacaatgcc agcttcagcg atgatgaaaa
     1621 gcgctctctg cacgcatatt taacgtggca gcttcatact ccggcgaata tgacgggaat
     1681 gaaaagccgg attgaattac tcggtgcagc ggaaaaatca gctgtgggta aggttatcgt
     1741 tagtgtcgct tgctctgacg gcagaattac gcctgccgaa atcaaacagc ttgagaagat
     1801 ttatacaagc ctgggactgg atccctcatc cgtatcaagc aatatccatc agcattccgc
     1861 aactgaaact gctctcgttt catctgtacc agctaatcag tcgacggcag ggtttacgct
     1921 ggatgccaac gtacttgccc gccacgaatc tgccactgat gatgttcgta aattactgaa
     1981 ctcaattttc actgaagaag aaccagaaca accagaaagc gctccagcct cattaaccgg
     2041 ggaaggggga cttgactccg ctcatagcca actttatcgc aggttgctgg agaaagaaca
     2101 atggtccaga aaagaggcga cagagttgtg tagtaactgg aacctgatgc tcggcggtgc
     2161 gcttgaggtg attaacgatt ggtcctatgc ggtggttgat gcaccagtgc tggaagatgc
     2221 tgatgatcac atttggattg acctggaaat agccaaagaa ttagagggtt agtgaatgtc
     2281 tgtaacacgt ataagagtga aagagcgcga cgctatcatt cagtcactta agtcgggagt
     2341 aacgccaaga ataggtattc agcatatcca ggttggccgc gtgaatgaga taaccgccct
     2401 ccatcaggat attgaacgga ttgctgatgg cggggcaagc ttcagactta tcattggtga
     2461 gtacggttcc gggaaaacat tctttttaag tgttgtacgg tctattgcgt tagagaagaa
     2521 gctggtcacg gtcagtgcag acctttctcc cgacagacgt atccactcat cgggaggtca
     2581 ggctcgtaat ctctactcag agcttatgaa aaatatgtcc accagaaaca aaccagatgg
     2641 caatgcatta cttagcgttg tggaaaggtt tgttacagtc aaggcagtca ccaaactcga
     2701 taataaagcg gctcatttcc atacgtctgt ccttaagggg cattgttcat ttctgtgacc
     2761 aagccgcacc acctttttta ctgattcgtc tgtcgggaac actttgcgtt tcttgatgac
     2821 ataacggatc acactattca acgactctat ggcattttgc acatacttcc atagcaactg
     2881 tcatcggtag ctactgaacc agcaagttaa gtaagttact acgtgaaaat tttatttttc
     2941 caacggtttt tccataatca ttaatattaa aaggataaaa atttgtttta gccagctcga
     3001 taccaatgat ttttatttca gacatgatat ccttcttaca tttcattgat taaagtttat
     3061 ccatcatggt atgatctgct tctaccgtga aggaggggct gacactcaat ggaccaaaaa
     3121 ctcacgttaa acaattgtag caaaggaaag ttaaagg
//
LOCUS       DAZKGF010000313         3069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZKGF010000313 DAZKGF010000000
VERSION     DAZKGF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.314"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..1481
                     /locus_tag="ACHOQV_005015"
     CDS             9..1481
                     /locus_tag="ACHOQV_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9633239.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAVLITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            1485..2315
                     /locus_tag="ACHOQV_005016"
     CDS             1485..2315
                     /locus_tag="ACHOQV_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9633240.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDSSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            2361..>3069
                     /locus_tag="ACHOQV_005017"
     CDS             2361..>3069
                     /locus_tag="ACHOQV_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFP9633241.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDVNKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKIN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFTPYIEYDYLDKQGVYK
                     GKDNKHENSYRVGVLINF"
BASE COUNT          826 a          504 c          740 g          999 t
ORIGIN      
        1 ggtttaatat ggactcctgt attcagaaaa agcaggtttg gtataagcat ctttcaccca
       61 gacagtggaa gatattcggt gcggcatgga caggttattt acttgatggt tttgatttcg
      121 ttctgatatc tctggttctt acagaggttc agcatgaatt cggcttgacg acaatagaag
      181 cggcaagcct tatttccgct gcttttattt cccgatggtt tggggggctt gctattggcg
      241 ctttatcgga caagatgggg cgcaggatgg caatggtttt aagtatagtg ctattctctc
      301 tcggaaccct tgcctgtggt ctggctcctg gatatgcagt gatgtttatt gctcgcattg
      361 ttattgggct tggtatggca ggtgaatatg gctcaagtgt aacatatgtt atcgaaagct
      421 ggcctgttca tctcagaaac aaggcgagtg gttttttaat ttctggattt tctattgggg
      481 gaggtttagc tgcacaggtt tatagcattg tagtaccact atggggctgg cgttctcttt
      541 tctttgtcgg catgcttcca atattatttg ctttttatct tcgcaagaat cttccggagt
      601 ctgatgactg gcagaaaagg caacaagaga ataaacccgt cagaacaatg gtggatattc
      661 tgtacagaga aaagaataaa tacataaata ttcttctgtc ttgtattgca tttgcatgtc
      721 tatatgtttg tttttcaggc gtcactgcaa acgctgtttt aatcacagtt atggcattgt
      781 gctgtgcggc tgtttttatc agttttatat atcagggtat ggggaaaaga tggccaacag
      841 gtattatgtt gatgcttgtg gttatgttct gtttcctcta cggatggcca ctgcaggctt
      901 ttcttcctac atggcttaag gttgatatgc agtattcacc agaaactgtt gctctcatat
      961 ttatgctggc aggttttggc tctgcagctg gaagttgtat aggtggtttt atgggggact
     1021 ggcttggaac ccgaaaagca tatgttatat cacttcttat cggtcagctt gttattatcc
     1081 ccgtttttct tgtggaccgg gactatgtat ggttacttgg ccttctgatc tttactcagc
     1141 aggtttttgg tcaggggatt ggtgctctgg tgccgaaaat aatcagtggt tattttaatg
     1201 tggagcagcg tgcggcagga ctggggttta tttataatgt aggttcactt ggtggagcat
     1261 gtgcgcctat tcttggtgca gttgttgcgt ctcatacaag tcttggtaca gcaatgtgtt
     1321 ctcttgcttt tatcttaaca tttgttgttc ttgtccttat tggttttgat atgccatcac
     1381 gtgtacagag atggatccat ccagaagctg cgcttgaata tgatactgtc gatggaaaac
     1441 ctttttatgg agcaagaaaa aagaatgttg caggagaata acggatgcta tcagggaata
     1501 aaaaaatgat tcattacagg gagaatatat attctcttga taattatgag cgttacataa
     1561 ctgacagttc gctatttgag aatactgacg atggcataat atatacaggg attgtgcccc
     1621 ttattacagt tgttgagcct gttgtatcta atcaacatag tgtgctgatt atacctggag
     1681 gtggatacag gaggattgtt tatgaaagag agggacttga tgtaggcaga tggatagcgg
     1741 gtcagggata tacggcattt attctttctt acagaatgcc ctccgttaat ggttattatt
     1801 cctttgcgga tgcgaagaat gccctgaact ttatttctta ctgggccgga atgtcggaga
     1861 gaacatttaa gagtgttgga tgtatggggt tttcggcagg tggtcatctt gctggatata
     1921 ttactcatct ccattatgag gaagagactt ctgtgcgtcc tgactggatg gctctgatat
     1981 atccagttat atctcttgac aggagtattg ctcatcaggg atgccggaaa cgtcttctgg
     2041 atgcaggtga aagtgaatat tcagccagtc ttgaaaatag tgttacagag tatatgccac
     2101 cagtctttat tgtgcatgca acagatgata aggatgttcc agttattcac tctctgagat
     2161 attttaactc tatccgggat aagggacatc atgcagaagc tcattttttt gataagggag
     2221 gtcatggatt tggtgtatcc ggaaaaaatg gatatccgat agaaaaatgg caaagcctgc
     2281 ttctggaatg gcttgcggaa cataagtttc attgagaaat cagattacaa gtgataaata
     2341 tatttaatga tgaggcattt atgaaaaaat attatccgct attaactgta attcttcttg
     2401 gtttaccttt gtgctctcag gcattaactc ttaatctacg tgggggatac agaagcgcca
     2461 gtcacgctta tgaaacgttg attcaggtta gtcaggggtg gaataatggt tggtgggcaa
     2521 gttttgagtc aaacacctgg aatacaattc acaacaatga aaatgaattg tttgctctca
     2581 attataataa aatagatgta aataaaacat ataaaataga tgacaaatgg gctctaatcc
     2641 cagggattca gacacaatgg atgtcagcag gaagtcagta taacccttat ctgaaaattt
     2701 cgtatcaggc aacgccagat cttaatctgg ccttacgcta tcgctatgac tggaaagctt
     2761 tccgacagac ggatttagat ggcaacaggg cgcgtaatga tcagcatcag tttgatggtt
     2821 atgtgacata caaaattaat gattcatggt tgtttgcatg gcagaccacg gtatatacga
     2881 aggttaataa tttcaaatat gggaatcata aaaaaacagc aactgaaaat gcatttgttc
     2941 tgcaatataa aatgtcgccg gtgtttaccc catatataga atatgattac ctggataaac
     3001 aaggggtgta taaggggaaa gataataaac atgaaaatag ttaccgtgtc ggtgttctga
     3061 ttaattttt
//
LOCUS       DAZKGF010000314         3055 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZKGF010000314 DAZKGF010000000
VERSION     DAZKGF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.315"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..340
                     /locus_tag="ACHOQV_005018"
     CDS             <1..340
                     /locus_tag="ACHOQV_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9633242.1"
                     /translation="YFSSPEALRFLWGVVDSPVYDIRHLTERCSVNDISHLYRAFMSV
                     EKLTLSGRQVNVLMMLLYGSNGTEGARYLGMSEKTFSTHKQNLIKRLRVHNEVELLYK
                     GMLLVRKGRL"
     gene            415..603
                     /locus_tag="ACHOQV_005019"
     CDS             415..603
                     /locus_tag="ACHOQV_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP9633243.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            747..1595
                     /gene="mscS"
                     /locus_tag="ACHOQV_005020"
     CDS             747..1595
                     /gene="mscS"
                     /locus_tag="ACHOQV_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP9633244.1"
                     /translation="MVRNQDMLPGYAANLVAAVVIIIIGGIAARMISGVLNNLMLGRN
                     IDATVALFLSALIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNL
                     AAGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIPNGKIIAGEIV
                     NFSREPERRNEFIISVSYDADIDCVKQVLTDIVMSEERVLKNREITVRLNEPGESSMN
                     FVVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSR
                     SVSSRQ"
     gene            1721..2941
                     /locus_tag="ACHOQV_005021"
     CDS             1721..2941
                     /locus_tag="ACHOQV_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFP9633245.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
BASE COUNT          766 a          568 c          835 g          886 t
ORIGIN      
        1 ttatttttct tctccggaag cgctccgttt tctctgggga gtggttgaca gtccggttta
       61 tgatatcaga catcttactg agcgatgttc agtaaacgat atcagtcatc tgtaccgcgc
      121 attcatgtcg gtggagaagc tgaccctgtc aggccggcag gtaaatgtac tgatgatgct
      181 gctttatggc agtaacggaa cagagggggc acgatatctt ggcatgtcag agaaaacatt
      241 ctccactcat aagcagaatc tgataaagag gttgcgggta cataatgaag ttgagttgtt
      301 gtacaagggg atgcttctgg tcagaaaagg gaggttgtga agcagggcag cttatttccg
      361 gtaatgttaa acgcagcctc tgattcgtta atttcataat cataaggagt ttttatgcgt
      421 ctgttgattg cttttcttct tccctggctg acttttttca ccattggccg gcctctgacg
      481 gggatttttt gtctggtgtt acagataacg ctgctgggct ggataccggc aacaatatgg
      541 gccgtgtatg cgctttcgca gtataagacg gaacagaaaa tccgcgaagc tacggggaaa
      601 tgatgactgc agaagagcag gttgtcatag ctgtgtatat gatgcgggat aaaggcccgg
      661 agattcctga aaataaaatg gttcgtggaa tataaaacag ggaaaaaacg gatgtgatgg
      721 acgcttctgg tgaggcggtt tcatgaattg tccgtaatca ggatatgctg cccggttacg
      781 ccgcgaatct tgtggcagca gtggttatta ttattatcgg gggtatcgct gcacggatga
      841 tttctggtgt gctgaataat ctgatgctgg gcaggaatat tgatgccaca gtggcgctgt
      901 tcctttccgc gctgatacgt catgccatta tagcctttac gctgattgct gccctgggac
      961 gtgtgggggt tcagacagct tccgtaattg cagtcctggg ggctgcaggt cttgctgttg
     1021 gacttgccct gcaggggtca ttgtcaaacc ttgcagctgg tattcttctc gtgatgttcc
     1081 gtcctttcag ggctggtgaa tatgctgacc tgggtggtgt tgccgggact gtgcagaatg
     1141 tacatatatt ctcaaccacg atgcgtacac tggatggtaa aattgttgtc attccgaacg
     1201 ggaagattat tgccggagag attgttaatt tctccaggga gcctgaacga cgaaatgagt
     1261 ttatcatcag tgtttcatat gatgcagata ttgactgtgt gaaacaagtc ctgacagata
     1321 ttgtgatgtc agaagagagg gttctgaaga acagggaaat cactgttcgc ctgaatgagc
     1381 ctggggagtc atccatgaat tttgtagtac gggtatggag caggcgtgat gaccttcagt
     1441 ctgtatactg ggatgtactg gagagaatta agcgggagtt tgattcagaa gggataagtt
     1501 ttccatatcc acaaatggat atccatcttg ttcgctccgg gaagcaggca gagtcggtaa
     1561 cccataacag tcgctcagtc agttccagac agtaaaatga aaagcgcatt ttatttgtga
     1621 aagatgaaca gcctgaatct gtcaggctgt tttcattgtg tttttatggt catttttggt
     1681 atatgtactg tatataatgt catctgtaca aggatataaa atgccaaaga aaataatgac
     1741 agggattgat gtttttgacg ctgccatgga gcgtattgaa tgggtgtttg atacgttttc
     1801 atcagtttgt ctttcttttt ccggcggaaa agactccact gtcctgtttc acctgatggc
     1861 gaatattgcc cgccgaaaaa aacgccgttt ttctgtactg tttgttgact gggaggctca
     1921 gtatcagtgt accattgagc atgtcaccag gttgagggaa atgtacgatg atatcacaga
     1981 tacttttttc tgggtggcgc tgcctctgac gaccgttaat ggagtgtctc aggtacagcc
     2041 tgagtgggta tgttgggagg acggggctga atgggttcgc cagccaccag aagatgccat
     2101 aaccagagcg tctttttttc ctttttatca gaatgcgatg acatttgaag agtttattcc
     2161 ggcatttaat cattggtttg cccgggaaaa agggatggtt gctgcgattc ttactggtgt
     2221 tcgcgctgat gagtcactga atcgttttat gggactggta tccggacgta agctccgtta
     2281 tgcggaggat aaaccctgga ccacggcatc ggctgaggga ttttactaca ccatgtatcc
     2341 tttgtacgac tggaaaacaa gagatgtatg gatttaccat gcgagaacac gtgctgccta
     2401 taactctctt tatgacctga tgtacagggc tggagttcct cttcgcaata tgcgggtgtg
     2461 tgagcctttt ggaccggagc aacgtaaggg gttgtggctt tatcatgtac tggagccgga
     2521 tacatggggc agaatgtgtg ctcgcgtatc aggtgctgta gggggagcgc tctatgcaaa
     2581 tgaaagtggt gcttattttg ctttgaggaa acagataaca aaaccagttc accatacatg
     2641 gcagagttat gcaatgtttc tgctggagtc aatgcctgag aaaacggcgg agcattacaa
     2701 gaataagatt gctgtttatc ttcggtggtg gcagtctcat ggatggccgg atggcatccc
     2761 tgatgagcag gataatgacc agggatgtaa agatattccc tcctggcgtc gcatctgtaa
     2821 gacgttactg aaaaatgatt tctggtgtcg gatgctggcg tttagtccga ataagccccg
     2881 acattatgag cgctacctgg ccagaatgcg tgaaaaaagg aaagagtggg gaatattgta
     2941 aattatattt aaacatcaga aatatgacgg ttaatccagg atagtagcta aaaaaataag
     3001 attccttgct gatttatccc tgtttgatat gtaaaatctg aggggcttat aagca
//
LOCUS       DAZKGF010000315         3003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZKGF010000315 DAZKGF010000000
VERSION     DAZKGF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.316"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(140..1654)
                     /locus_tag="ACHOQV_005022"
     CDS             complement(140..1654)
                     /locus_tag="ACHOQV_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP9633246.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPPFNPLVDREPGPVMVFNGEKNVPADSLKSQRLIYPDSNNHTGK"
     gene            complement(1806..2546)
                     /locus_tag="ACHOQV_005023"
     CDS             complement(1806..2546)
                     /locus_tag="ACHOQV_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFP9633247.1"
                     /translation="MNKVIAVSALAMAGMFSTQALADESKTGFYVTGKAGASVMSLAD
                     QRFLSGDGEETSKYKGGDGHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTG
                     ISTWDYGYGSSGRESLSRSGSADNFAWSLGAGVRYDVTPDIALDLSYRYLDAGDSSVS
                     YKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            2770..2955
                     /locus_tag="ACHOQV_005024"
                     /pseudo
     CDS             2770..2955
                     /locus_tag="ACHOQV_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          866 a          729 c          665 g          743 t
ORIGIN      
        1 tcaatatcgt actcaaactc atcaaatagc tgtaatccgg tcagattatt tctctcccac
       61 attgttgtac aactcaactt taataaaatt attataaaaa gcaggaatat atcatttacc
      121 agtcatcgtc agataccatt cacttacctg tatgattatt actgtcagga taaataagtc
      181 tctgtgactt aaggctgtct gcaggaacat ttttctcgcc attaaacacc ataaccggcc
      241 ccggctctct gtccacgagc gggttgaatg gcggtatatt ttcagtacga atgccggtca
      301 gccactgaaa aatacctgcg aaatgacggg cactgatttt cctgctgacg gactgatgag
      361 acgtgatgtc actggcggta ataatcaggg gaacgctgta gcctccctgc acatgaccat
      421 catgatgaac aggattggca ctatcgctga ccgacagccc atggtcagaa aagtaaagca
      481 tggcaaaatg acgggaatgc cggcgaagga taccatcaag ctgaccgaga aagttatcca
      541 gtttactgat gctggcgagg taacaggcaa tctttcgggg atactgctcc aggtaatgat
      601 tcggccagga gttaagtcgg tcacacggat tcggatgaga gccaatcatg tgcaggaaaa
      661 tcaccttcgg agaggattta tccgccagcg cacgttctgt ttcctgtaac aacaacatgt
      721 catccgtttt acgggaagcg aagctgcctt tcttgaggaa aacggtatgc tccgcatcag
      781 aagcaataac agagatgcgt gtgtcatgct cccccagttt tccctgattg gatatccacc
      841 atgtgctgta tcctgctttt gctgccagcg ccaccacgtt gttgccggag tcagggttct
      901 gctcatagtc ataaatcagt gtccggctca gggaaggtac ggtactggct gctgccgatg
      961 tatagccgtc aataaataaa ccgggagccg tattcagcca cggtgtggtt ggtacgggat
     1021 aaccatatac cgacatataa tccctgcgca cactctcacc agtgacaatc acaatcgtgt
     1081 catacaacgg tgctcccggc aggattttcc agctgtcagc cctgtgctga ctcagttgtt
     1141 cataacgctg catttcacgc aatgtgtcag ttgtccccac aacggttcct ttaaccatcc
     1201 gcaacggcca gctgtttaca gagcataata cgaacagcag cagtgccagc cagttacggt
     1261 gaccgcggtg gtgtgtccgc cagaaaataa ccatgaacac cagaatcgca gcactgacca
     1321 gaaaatgata aacaggaatc atcccggtaa actccgcagc ctcatctgcc gtggtctgca
     1381 acaacgcgac aataaaactg ttgttgattt taccgtacgt catacccgca ggcgcataca
     1441 gtgcacaaca gaacagaaat aacagagctg taatggatgt aagggtattt ctgtgtgcaa
     1501 ggagcagaag aaggaacagc agcaacacat tcccggtggc attcttctca gtgtatccgc
     1561 atgcaattgt ggttatgaca gaaataacaa aaaagaataa aaacaataaa atccgtggtg
     1621 tgttgtccgg acaaaacagt tttctgatat tcatcggagt atatcgacaa cattattgtg
     1681 aagagaataa aaatcagaag tctcctgtga aacagataac aaacaaccct gcagtataat
     1741 tactgcaggg agtcttcgtt taattacaga agatgggtaa ttattatctt tcaggagcag
     1801 ttttgtcaga aattataagt cacaccaagc atgatgtcat gacttttaac atcaacttct
     1861 gatttatatt tatcgcccca ctcgtccttg taactcacac tgctgtcacc tgcatcaaga
     1921 tagcgatagc tgaggtccag agcgatatcc ggggttacgt catagcgaac acccgcgcca
     1981 aggctccatg cgaagttgtc agcagagcct gaacgtgaca acgattcgcg accactgctt
     2041 ccgtacccat aatcccaggt actgataccg gttgttttct ggtgaattct ggcgtagcca
     2101 atccctgcag atacccatgg tgtgaatgca ctgtcattcc ggaagtcata gtacgcattc
     2161 agcatcagtg tgttgactga cacctcattc ttcaggtcat cacgccagta accgcctgac
     2221 cagctgtctt tatctacgtt atacttcgaa tcagcttttc cacgagcgta aaactccagt
     2281 tccgtacgaa ccggaatact gaactgcggg taaaaatcat aaccggccgc gataccgcca
     2341 ctgaataccg tatcatggcc atcaccgcct ttatattttg atgtttcctc tccatcaccc
     2401 gacaggaaac gctggtctgc aagtgacata acagaagcac ctgctttacc ggtcacataa
     2461 aagcctgttt tgctctcatc agccagagcc tgggtcgaaa acatgcctgc catggcaagc
     2521 gctgaaaccg caataacctt attcatttca atcatctccg ctatcacttt ccccgaacga
     2581 taaatggggt tgctcattct aaaaccatgc aaaagacagg gtaaaaggaa tgttttcgat
     2641 cgaatcgatc gataaaatcg atcgtgcccg ataaagtttt cttatcgtcc ccataaaaat
     2701 ggtaagggct ggtttaagag gtaatcgcag cggcatggaa gtattaagac caaaacagca
     2761 cgagataaag cggctgaacg tcagatgagc cacatggaac gtaattcatt aagggcggct
     2821 tatatccata aaacagaaca cctggaatag cgtcggttga tgctacagtg gtggggggat
     2881 tttctggata caaactcgaa agaaaatacc agcccctttg agtatgcgaa gattaacaat
     2941 ccattaaaat attaagcact ccgggcaatc tgcccggagt gctttagctg gcataacggg
     3001 cta
//
LOCUS       DAZKGF010000316         2970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZKGF010000316 DAZKGF010000000
VERSION     DAZKGF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.317"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..390
                     /gene="ompT"
                     /locus_tag="ACHOQV_005025"
     CDS             <1..390
                     /gene="ompT"
                     /locus_tag="ACHOQV_005025"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP9633248.1"
                     /translation="AIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVEASDNDEH
                     YDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRVTNKKGDTSLYDHND
                     NTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            622..1290
                     /locus_tag="ACHOQV_005026"
     CDS             622..1290
                     /locus_tag="ACHOQV_005026"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9633249.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSA
                     SGKK"
     gene            complement(1372..1714)
                     /locus_tag="ACHOQV_005027"
                     /pseudo
     CDS             complement(1372..1714)
                     /locus_tag="ACHOQV_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            1727..2322
                     /locus_tag="ACHOQV_005028"
                     /pseudo
     CDS             1727..2322
                     /locus_tag="ACHOQV_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            2807..>2970
                     /locus_tag="ACHOQV_005029"
     CDS             2807..>2970
                     /locus_tag="ACHOQV_005029"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HFP9633250.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEG"
BASE COUNT          933 a          653 c          611 g          773 t
ORIGIN      
        1 gcaatcggct acaaacaacg ttttaaaatg ccctacattg gcttgactgg aagttatcgt
       61 tatgaagatt ttgagctagg tggcacattt aaatacagcg gctgggtgga agcatctgat
      121 aacgatgaac actatgaccc aggaaaaaga atcacttatc gcagtaaagt caaagaccaa
      181 aattactatt ctgttgcagt caatgcaggt tattacgtaa cgcctaatgc aaaagtttat
      241 attgaaggcg catggaatcg ggttacgaat aaaaaaggtg atacttcact ttatgatcac
      301 aatgataaca cttctgacta cagcaaaaat ggtgcaggca tagaaaacta taacttcatc
      361 actactgctg gtcttaagta caccttttaa caacgttaaa tagattttct ccccggtggt
      421 attgtatagc cccggggaat ttgtttaccg ttaaaagcaa acaatatccc ctaatcttgt
      481 aagcatttaa cctgcgtcct ggcgtgtaac ctcttaccgc aacacaagac tcaatatatt
      541 caaattcgtt atgccgtggc atcatttaac cccttgtaat tcatcgctat aattgattta
      601 attcacaaat aaaactataa catggtgaaa ctaatgaaaa aaaacacaga tgatggggct
      661 aaaatttaca caccacttac cctaaagctt tatgactggt gggttttggg tgtatcaaat
      721 cggcttgcat ggggatgtcc tacaaaggaa caccttcttc cacactttct ggaacattta
      781 ggtaacaacc atctggatat tggcgttgga actgggtttt accttactca cgtacctgag
      841 agtagtctga tatctttaat ggatttgaac gaagctagcc tgaacgcggc atcgacaagg
      901 gctggggaat caaaaattaa acataaaatt agccatgatg tttttgatcc ttatcccgcg
      961 gcgttacatg gtcaatttga ttccatttcc atgttttacc ttcttcactg cctgcctgga
     1021 aatatatcta caaaaagctg tgtaatacgc aatgcggcgc aggccttaac tgacgatgga
     1081 actctatacg gagccacaat tcttggtgac ggcgttgtgc acaatagctt cggtcaaaaa
     1141 ctgatgcgca tttacaatca gaaaggcatc ttttcaaaca caaaagattc cgaagaaggc
     1201 ttaacacata tactctcaga gcatttcgag aatgttaaaa ccaaggttaa aggtactgta
     1261 gtaatgtttt ccgcttcagg gaaaaaatag catccagccg cagcacgttc ttgcgcacga
     1321 cgttctgcgg cataatccca atgattactc tctgacaggg ttcacaggcc actaaatatc
     1381 aggggcagtt gatgtatcaa ctcggttcag caacacccga tacatcttcc aggcttccag
     1441 caacgaggtt tcttcctccg tatcctttat ccatgatatg ccgttccact tctgaaactc
     1501 ccctgccggg gatacctggt gacattttcc ggtaacggac cgagttcaga aataaataat
     1561 gcgtcccctg atgccacgtc ataaaccgtt ttaccccgat ggtcttcaac gagatgccac
     1621 gatgcctcat cactgttgaa aacaaccacg aagccagccg gaatatctgg gcaatatcag
     1681 tactgtttgc aggcaaacct gtatgaggtg gaatggcaca agagacttac aaaagaatca
     1741 ggccaaacgc ctgattgatg agagccccga accactgaaa tctgtcgtca aatttgcgct
     1801 ggtaactgtt ctgagaaagt cgaacatcat aaatctggag tggcaacaga ttgacatgca
     1861 gcgacaagtt gccagggtga atccagaaga tagcaaatca aactgcgcca ttggtgtggc
     1921 gctgaacgat accgcctcta aagtgttgcg tgatcaaaca ggcaagcatc acaaatgtgg
     1981 gtgtacatac cagggcggct aagcgagcag atggaacatc aatgcctgcg ctcaggaaga
     2041 tgcgcattga cagcaagaca gcatggctat cagcttgtcg tcgtgcagga attgaaaatt
     2101 tccgtttcca tgacctctgg catacctggg caagctggtt gattccgtca ggcgtcccat
     2161 tatcagtgct tcaggaaatg ggcggatggg agtccagaga aatggttcgt agatatgctc
     2221 accttgcgcc taatcatttg actgagcacg cgaggaaaat agacgacatt tttggtgata
     2281 atgtcccact ctggaattat agaagaaata aagaaggcgt aactgattga attttaatgg
     2341 cgcgccctgc aggattcgaa cctgcggccc acgacttaga agttcctaga acgacatttt
     2401 aagtcaacaa cttaccgcgc catctctgcg ctcacacgtc ccactacctc aaaacatgta
     2461 aagccttgca agccattgcg aggccttatg tgtctcagtt ttgtccctct tttttgtact
     2521 aaaaaacata gtaattgagg ataaacctca tgctattttc gcttatatgc ctctaaaggc
     2581 atggcactta aatagataaa agcaccacaa aagcataaaa aaaaccacac agtaaaaccg
     2641 caatatgaaa caataacaga taattaaacc aaaaacagat agcgcattgt gataatcatt
     2701 caatactaaa caaaatataa acagtggagc aatatgtaat tgactcatta agttagatat
     2761 aaaaaataca tattcaatca ttaaaacgat tgaatggaga acttttatgc gggcgaaact
     2821 tctgggaata gtcctgacaa cccctattgc gatcagctct tttgcttcta ccgagacttt
     2881 atcgtttact cctgacaaca taaatgcgga cattagtctt ggaactctga gcggaaaaac
     2941 aaaagagcgt gtttatctag ccgaagaagg
//
LOCUS       DAZKGF010000317         2955 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZKGF010000317 DAZKGF010000000
VERSION     DAZKGF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.318"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            309..566
                     /locus_tag="ACHOQV_005030"
     CDS             309..566
                     /locus_tag="ACHOQV_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFP9633251.1"
                     /translation="MLHIIRKGDKTTHGGSVLTASETMKFGGIGVARKGDKVSCPGHG
                     PTIIIEGNPDYLDHGIPVAFHGHKCACGCTLISSFAAAKVA"
     gene            569..1777
                     /locus_tag="ACHOQV_005031"
     CDS             569..1777
                     /locus_tag="ACHOQV_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633252.1"
                     /translation="MPVWLDAIPEKAPKIARPNTRRWLLFLAFIMLGGMALTLWCWTS
                     ERTGFVFWFTALGLPFCTWGLIFGLRRFVYKAGQVGAESRNVEREALIDSEILRGQRC
                     AWILGTYIQSPAGNKADDLLEAMEVAAPAIDFSHPRGCDKPVRYAALPEYQTDLTKAL
                     KGAVNKLTTRVEGIVHPLPPALPCWLVLDCDNDLYPLIEEQLKADLSLKTGRIFRLMT
                     GKGLGAFDAWLDKRWDTPGILVVITLSLPASPREEDADAVSMVVLSNRNTHAWPDALC
                     LHRPERGTETTLTKTLTRAFLWAKTRPNELKGSWISGPTLTSGSSWNNACEENGVTFS
                     LSEDNFSIDPVLGYTGHAAPWLAITLADAAFEQRGAQVIAAQPTADKDDVWVAVITKE
                     EVRKESPGNG"
     gene            1770..>2955
                     /locus_tag="ACHOQV_005032"
     CDS             1770..>2955
                     /locus_tag="ACHOQV_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HFP9633253.1"
                     /translation="MAKVKVKTLIGSVVTVAFFLTVIVAFLFYAFPENVASATGFGPY
                     DGLRILTFCAILVATLFLLGWLTEKAFDFAGKSGLYFQWGQKKAESITPVTNTIPGEE
                     DDDEPVFSEDAVSEHLRFRYGRRWQRKVRLLLVMGDPDVVRKAAPGLCHDLWQEGDGY
                     VLIYGGDAQSLPDEIFLSKLKRLRSGQPVDGIIQVMSISALLTDGERDALLRYRQKAD
                     HQLGWQAPVWLWLTDNATGARQDAKAAATGVIFGPEGTVNGADEAFSTLALHLQKAGM
                     AQILNEPADFGLLQLSLRLRQELKASLAVLLSGLMQGTAAWRLRGVMFSPELTVAGAV
                     PNTCPDTPAWKAIIDDCDAVSGRKLGFNWLKMLRLILLSLIVLWGAGTLLSLLVNRTQ
                     IYQ"
BASE COUNT          695 a          718 c          820 g          722 t
ORIGIN      
        1 aagaatgttt gtggtataaa aattaatgac gagtttgtag agatgtagat agttctaaat
       61 gtgaatttta ttgcgcacta tctgtagtat cgcattcata tttgtatgaa attggcaaaa
      121 aataatagta tttattgata actcaatggt aaatacatgc gatggggaac actatctatc
      181 aataatcagt gacccggaaa cattcataat aaaaatccag acaatcacta tcgatggcag
      241 agcccagcat aaacatggga attaaagagt gatgaattag ttaagagata agaaagagga
      301 atttaataat gcttcatatt attcgtaaag gcgataaaac tactcacgga ggttccgttc
      361 tgaccgcttc tgaaacgatg aaattcggtg ggataggcgt cgcccgtaaa ggcgataaag
      421 tgtcatgtcc gggtcatggt cctaccatca tcatagaagg caatcctgat tatcttgacc
      481 atggtatccc ggtggcattt cacggtcata aatgcgcctg tggttgcaca ttgatcagtt
      541 catttgccgc agcaaaggtt gcctgattat gcctgtctgg ctggatgcca tccctgaaaa
      601 ggccccaaag atagcgcgcc ccaatactcg ccgctggctg ttgttcctgg catttatcat
      661 gctggggggg atggcgctca cattatggtg ctggacgtca gaacgaacag gatttgtctt
      721 ctggttcacc gctctggggc ttccattttg cacgtggggg cttattttcg gtttacgtcg
      781 gtttgtctat aaagccgggc aggtgggggc tgagtcccga aatgtcgagc gggaagcgct
      841 tattgacagt gaaattctgc gtggtcagcg ctgtgcctgg atcctcggta cttatattca
      901 gagcccggcc ggaaacaaag ccgatgacct gcttgaggca atggaagtcg cggctcctgc
      961 tattgatttc tcgcacccaa gaggatgtga taaaccggtc aggtacgcgg cattaccaga
     1021 ataccagaca gatttaacga aggctctgaa aggggcggtg aataaactga ccacgcgtgt
     1081 agaggggata gttcatccgc ttccgcccgc gctaccatgc tggctggtgc tggattgtga
     1141 taacgatctg tatcccctga ttgaagagca gcttaaagcc gacctctcac tcaagacggg
     1201 caggattttt cgcctgatga ccggaaaagg gctgggggcg tttgatgcct ggctggacaa
     1261 gcgatgggac accccgggca ttctggttgt catcacgctg tcgttacctg cctctccccg
     1321 ggaagaagat gctgatgctg tctcaatggt ggtattaagc aatcgcaaca cccatgcctg
     1381 gccggatgcg ctttgtcttc atcgacccga aaggggaaca gaaacaacgc tcacaaagac
     1441 acttacccgg gcgtttctct gggcgaagac ccgacccaat gagcttaaag gaagctggat
     1501 ctccggtccc acattgacgt cgggcagcag ctggaataat gcctgcgaag agaacggagt
     1561 gacattcagc ctttcagaag acaatttcag tatcgatccc gtgctgggct ataccggcca
     1621 tgctgctcca tggcttgcca tcacgcttgc tgatgctgcc tttgaacaac gaggtgcgca
     1681 ggttatcgcc gcccagccca cagctgacaa agacgacgtc tgggttgccg tcatcactaa
     1741 agaagaagtt cgcaaggaat cacccgggaa tggctaaggt aaaagttaaa acactcatcg
     1801 gctctgttgt gactgtcgcc ttttttctga cggtcatcgt ggcatttctt ttttatgcct
     1861 ttccggaaaa cgtcgcttcg gccaccggct tcggtccgta tgacgggctg cgcatactca
     1921 cattctgcgc catactggtt gccacgctgt ttttactcgg ctggctgacg gagaaggctt
     1981 tcgacttcgc cggtaaatcc gggctgtatt tccagtgggg gcagaaaaag gctgagagta
     2041 tcactccggt gacgaacacc attcccggtg aagaagacga cgatgagccg gtttttagcg
     2101 aagatgctgt ttcagaacat ttgcgctttc gctatgggcg tcgctggcag cgtaaggtgc
     2161 gactcctgct ggtgatgggg gatccggatg tggtccggaa agcagccccg ggcctttgcc
     2221 atgacctctg gcaggaaggc gacggctacg tgctgattta tggcggagac gcgcagtcac
     2281 tcccggacga gatcttcctt tcaaaactga agcgtctgcg atccggtcaa ccggttgatg
     2341 gcatcattca ggtgatgagt atttcagccc tcctgacgga tggcgaacgg gatgccctcc
     2401 tgcgttaccg tcagaaagcc gaccatcagt tgggctggca ggcaccagtc tggctgtggc
     2461 tgactgacaa cgctaccggt gcccggcagg acgcgaaagc ggccgcaaca ggggtcatct
     2521 tcggaccgga aggtaccgtg aacggtgcgg acgaagcgtt ttccacactg gcgttacacc
     2581 tgcaaaaagc aggaatggcg cagatactga atgaacctgc tgatttcggt ctccttcagt
     2641 tgtcactcag actgcggcag gagctgaaag ccagcctggc tgttttgctc agtggactga
     2701 tgcaggggac tgctgcctgg cgtttacgcg gtgtcatgtt cagtcctgaa ctgaccgttg
     2761 ccggagcggt accaaacaca tgccccgaca cacctgcctg gaaagctatt attgatgact
     2821 gtgatgccgt cagcggcagg aagctgggct ttaactggct gaagatgctg cgattgattc
     2881 tgctttccct gattgtcctg tggggggccg gtacactgct gtctctgctc gttaaccgta
     2941 ctcagattta tcagg
//
LOCUS       DAZKGF010000318         2927 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZKGF010000318 DAZKGF010000000
VERSION     DAZKGF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.319"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..378
                     /gene="tssE"
                     /locus_tag="ACHOQV_005033"
     CDS             <1..378
                     /gene="tssE"
                     /locus_tag="ACHOQV_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP9633254.1"
                     /translation="EEEQVILSVLDNMQRILNTRAGSLKHLPDYGLPDMTSILQGMPG
                     TAHQLMRVLSDVLLKYEPRIKRVGVTMQEQTQPGELHYVIDAELKDAGLVRYGTTFMP
                     EGRVLLRYLKQQQFVDNVMTFRG"
     gene            431..736
                     /locus_tag="ACHOQV_005034"
     CDS             431..736
                     /locus_tag="ACHOQV_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633255.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            791..2155
                     /locus_tag="ACHOQV_005035"
     CDS             791..2155
                     /locus_tag="ACHOQV_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFP9633256.1"
                     /translation="MTSEVKLKTGGDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTVSWYTIARMHTAGLSGLNEGLALIVALTRHHWSVMWPLNTHAR
                     LEIITGLFSRLQKTLRAMPPDERDNLPLLYQTETCLKTLSDILARHELKQSSKVALPE
                     AMVKGYITRLENQPVQGEVSSPVTLPAQALRSDAPDVQEHQPLPYSRLVYVVSETKTE
                     PTLFLQKSPLTFLKTFVTGVCTALLAVCVAIPGWQFLTQPSPAEQQLRALLAPLPDVL
                     SADQMAQLSRTPSLLNHTSEWITLSGQQITRLTELPPAYNLQRSAQLLQQLRGLFPDN
                     PRVQEMVDNWHKSVRSRALPEEAMTGWNEGMARLQQLAERLNRLDEQRGKYMTVSELK
                     TEVFGIMQAFNRHIPAEEQLRRYDEARNQNGSEQQQKQAEMALNQLINRYQMENAGKP
                     KSQP"
     gene            2171..2803
                     /locus_tag="ACHOQV_005036"
     CDS             2171..2803
                     /locus_tag="ACHOQV_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFP9633257.1"
                     /translation="MEMENTPGRARTPWTIEDVRYLETHYGVKKTREIAAYLNRSATA
                     VRAMAYLVGITTPVAADWSETEIEIIRRDYPAGVALADICARLNGRAPKSVCWMARRF
                     GLKRLGYWSAEEDEILRQYYPSEGVKVSRRLSGRTENAVKLRARELGVKFTGNGRLRL
                     WTAEEWNVLSGNMASGLSALMKLLPGRSRSSVQGAMRRLQKMSAQKSVRQ"
BASE COUNT          764 a          682 c          821 g          660 t
ORIGIN      
        1 gaggaagagc aggttattct ttcggtgctc gataacatgc agcgtattct gaatacccgg
       61 gctggaagcc ttaaacatct tccggattat ggtctgccgg atatgacaag cattctgcag
      121 ggaatgccgg gaacggcgca tcaactgatg cgtgtgcttt cagatgttct gctgaagtat
      181 gagccacgta ttaagcgtgt cggggtgacg atgcaggagc agactcagcc tggtgagttg
      241 cattatgtca ttgatgcaga acttaaagat gcaggactgg ttcgttacgg aacaactttt
      301 atgccggagg gcagggtgtt actgcgatat ctgaagcaac agcagtttgt ggataatgtg
      361 atgactttca gggggtgatt tatggttccc cttatccgtg tgcttaacag acataaacga
      421 gggctgaatg atgaacccgt tatttaatga catccagatg cgcctttttt atctgaatca
      481 tgctccgtat tcctggcact ggaatgtcag attcaggcca caggaagccg tatatattgg
      541 taatgatgca tgccatatta ccattacctg taatcagtcc ggttttcatt taaccaggga
      601 cgggcagcga ctgttcactg aacgttatat cagaacactc agtgagttat tagcggttct
      661 gaagcggcgg tgggatgtga caccggcaat catccgtgcg gtggaatacc tttcccgggt
      721 accggtatca cactgatgtc ctttccgtaa ctgttaatca tgatttaata cagcaacacc
      781 gaatctgccg atgacttctg aagtgaaact gaaaaccggt ggtgaccccc ggtcattacc
      841 ggattatgct gccctgcgtg atgaaatcag caaactgacc cacccggcac gtccggatgt
      901 ggactggcgt tacgtggaaa cgctctgcct gcgtctgtat gaacacaatg gcgtggaatt
      961 acagacggta tcctggtaca ccattgcccg gatgcataca gcagggttaa gtggtctgaa
     1021 tgaagggctg gctctgattg tggcgctgac ccggcatcac tggtcagtga tgtggccgct
     1081 gaatacccac gcccggctgg aaattattac cggtctgttc agtcgtctgc aaaaaacgct
     1141 ccgggccatg ccaccggatg agcgtgacaa cctgcccctg ctgtatcaga cagaaacatg
     1201 cctgaaaacg ctgagtgaca tactggcccg gcatgaactt aaacagagca gtaaagtggc
     1261 tttaccggaa gccatggtga agggatatat cacccgtctg gagaatcagc ctgtgcaggg
     1321 tgaagtatcg tcgccggtaa cccttccggc tcaggcactc cggtctgatg ctccggatgt
     1381 acaggaacat cagcccctgc cgtactcccg tctggtgtat gttgtttctg aaacaaaaac
     1441 agagcccaca ctcttccttc aaaaatcccc gctcacgttt ttgaaaacgt ttgttacagg
     1501 tgtctgtacg gcattgctgg cggtctgcgt ggccattccg ggatggcagt ttctgacaca
     1561 gccatcaccg gcggagcagc aactcagggc gctgctggca ccactaccgg atgtgctttc
     1621 agcagaccag atggcgcaac tcagcagaac accctcactg ctaaatcata cgtcagagtg
     1681 gataaccctt tccggtcagc agataacgcg actgacagaa ctaccgccgg catacaacct
     1741 gcaacgcagt gcgcagctgc tgcagcaact gaggggactt ttccccgaca atccccgtgt
     1801 tcaggaaatg gtggataact ggcataagag cgtaaggagc cgcgcgctcc cggaagaggc
     1861 gatgacgggc tggaacgaag gcatggcccg tttacagcaa ctggctgagc gcctgaaccg
     1921 tctggatgaa cagcggggaa aatacatgac ggtcagtgaa ctgaaaacgg aggtgtttgg
     1981 catcatgcag gcttttaacc ggcacatacc ggcagaagag cagttacgtc gttatgatga
     2041 agcccgtaac cagaatggca gtgaacagca gcaaaaacag gctgaaatgg cgctgaatca
     2101 gttgattaat cgttatcaga tggagaacgc aggcaaaccg aagagccagc cataatggct
     2161 gagataaaac atggaaatgg aaaatacccc cggaagagcg cgcacaccat ggacaatcga
     2221 agatgttcgc taccttgaaa cgcattatgg cgtgaagaaa acccgcgaaa tagcggcata
     2281 cctgaacaga agtgcgacag cagttcgtgc catggcgtat ctggtgggga taacaacacc
     2341 agtggctgcg gactggtcag agacagagat tgagattatt cgcagggact atccggcagg
     2401 cgttgcgctt gctgatattt gtgcccgtct gaacggacga gcgccgaaat ccgtgtgctg
     2461 gatggcccgc aggtttggtc tgaaacgcct gggttactgg agtgcagaag aagatgaaat
     2521 cctgcggcaa tattatcctt cagaaggtgt gaaagtgagc cgacgccttt ccggacgtac
     2581 agaaaatgca gtgaaactga gagcgcggga gctgggtgta aagttcacgg gaaacgggcg
     2641 attacgtctc tggacggcag aggagtggaa tgtgttgtcc ggaaatatgg catcggggct
     2701 atcggcactg atgaagttac tgccggggcg ttcccgtagt tcagttcagg gggctatgag
     2761 gcgtttacag aaaatgagtg cgcagaaatc tgtcaggcag tagccaaaat attcctgcat
     2821 caggagaacg gcagtggcaa taataagcag caaactgata cataatgcag cttctgtaaa
     2881 cggtaatgat caagtaggga tatttttatg ttgactggtt gtgaaaa
//
LOCUS       DAZKGF010000319         2916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZKGF010000319 DAZKGF010000000
VERSION     DAZKGF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.320"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..667
                     /locus_tag="ACHOQV_005037"
     CDS             <1..667
                     /locus_tag="ACHOQV_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP9633258.1"
                     /translation="GTREVRRFRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILP
                     ELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPS
                     PAQAQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRL
                     QDELCTESVRGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDTVVSVARAVWNW
                     IFF"
     gene            1039..>2916
                     /locus_tag="ACHOQV_005038"
     CDS             1039..>2916
                     /locus_tag="ACHOQV_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP9633259.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SM"
BASE COUNT          684 a          755 c          874 g          603 t
ORIGIN      
        1 cggcacccgg gaagtacggc gcttccgtct gagtggccac ggtcacagca tggttatcac
       61 tgacctgccc ggggtgggcg agagccggga cagggatgca gagtatgagg ccctgtaccg
      121 tgacattctg cctgaacttg acctggtact gtggctgatt aaagccgatg accgtgccct
      181 gtctgtggat gagtatttct ggcgacacat cctgcaccgg ggacatcagc aggtgctgtt
      241 tgtggtgacg caggccgaca aaacggagcc ctgccatgaa tgggatatgg ccggcattca
      301 gccttctccc gcacaggcac agaacattcg cgaaaaaaca gatgcggtat tccgtctgtt
      361 ccggcccgta catccggttg ttgccgtatc ggcccgcacc ggctgggagc tggatacgct
      421 ggtcagtgcg ctcatgacag cgcttcccga ccatgccgcc agtcccctga tgacccgcct
      481 gcaggacgag ctgtgcacgg aatctgtccg ggggcaggcc cgtgaacagt ttaccggtgc
      541 ggtggaccgg atatttgaca cggtggagag cgtctgtgtt gcctctgttg cacgcacagt
      601 tctgcgtgcc gtccgtgaca cggtggtctc tgttgcccgc gcggtatgga actggatttt
      661 cttctgaaca gtctgccagg ggaaatgtct gattacattg cgttaaccaa ctgaaaacat
      721 gatacaaaca cctcatgccc ctcctgtttc tcttgcgtga ctgctctact gttaatagaa
      781 taaaacgatc gataaaacag gtatcgatcg tttatatcga tcgtttatag tggttaatgg
      841 cctgcgaaaa ggcagctgaa tctcttcatc atgcagaacg gaattgcaca caacagactg
      901 atacttctct gtctgatgaa gggggagagc gtttacgccc gggacgtatc gctgtgcccg
      961 ataactctgt tttgtacctg ccggtatcca cttttgtggg taccggcttt tttattcacc
     1021 ctctgtaagg aaaagctgat gaaacgacat ctgaacacca gctacaggct ggtatggaat
     1081 cacattacgg gcaccctggt ggtggcctcc gaactggccc gctcacgggg aaaacgcgcc
     1141 ggtgtggcgg ttgcgctgtc tcttgctgct gtcacatcag tcccggcact ggctgctgac
     1201 aaggttgtac aggcgggaga aaccgtgaac gatggaacac tgacaaatca tgacaaccag
     1261 attgtcttcg gtacggccaa cggaatgacc atcagtaccg ggctggaact ggggccggac
     1321 agtgaagaaa acaccggtgg gcaatggata cagaatggcg ggatagccgg aaacaccact
     1381 gtcaccacaa atggtcgtca ggtcgtgctg gaggggggaa cagccagtga tacggttatt
     1441 cgtgacggcg ggggacagag cctgaacgga ctggcggtga acaccacact gaataacaga
     1501 ggcgagcagt gggtgcatga gggcggggtt gccaccggta caattatcaa ccgcgacggt
     1561 taccagagcg ttaaaagtgg cgggctggca acaggaacca tcatcaacac cggcgcagaa
     1621 ggcggccctg attctgacaa ctcgtatacg ggtcagaagg tccagggaac agcagaatcc
     1681 accaccatca acaaaaatgg acggcagatt atcttatttt ccgggctagc ccgtgacact
     1741 ctcatttacg caggtggtga ccagtcggta cacggaaggg ccctgaatac cacactgaat
     1801 ggcggttacc agtatgtgca cagggacgga cttgcgctga acacggtaat taacgagggg
     1861 ggctggcagg ttgttaaggc aggtggcgct gccggtaaca ccaccataaa tcagaacggt
     1921 gaactgaggg tacatgccgg cggggaagcc actgcagtca cccagaacac gggcggtgca
     1981 ctggttacca gtactgctgc aactgtcatc ggcacaaacc gtctggggaa tttcacggtg
     2041 gaaaacggta aggctgacgg tgttgttctg gaatccggcg gtcgtctgga tgtactggag
     2101 agccattcag cacagaatac cctggtggat gacggcggta ccctggcagt gtctgccggc
     2161 ggtaaggcga caagtgtcac cataacatcc ggtggtgccc tgattgcaga cagtggtgcc
     2221 actgttgagg ggaccaatgc cagcggtaag ttcagtattg atggcacatc cggtcaggcc
     2281 agcggcctgc tgctggaaaa tggcggcagc tttacggtta atgccggggg acaggctggc
     2341 aacaccactg tcggacatcg tggaacactg acgctggctg ccgggggaag tctgagtggc
     2401 agaacacagc tcagtaaagg cgccagtatg gtactgaatg gtgatgtggt cagtaccggc
     2461 gatattgtta acgcagggga gattcgcttt gataatcaga cgacaccgaa tgccgcgctg
     2521 agccgtgctg ttgcaaaaag taactccccg gtaacgttcc ataaactgac caccacgaac
     2581 ctcaccggcc agggcggcac catcaatatg cgtgttcgcc ttgatggcag caatgcctct
     2641 gaccagctgg tgattaatgg tggtcaggca accggcaaaa cctggcttgc gtttacaaat
     2701 gtcggaaaca gcaacctcgg ggtggcaacc accggacagg gtatccgggt tgtggatgca
     2761 cagaatggcg ccaccacaga agaaggtgcg tttgccctga gtcgcccgct tcaggccggc
     2821 gcctttaact acaccctgaa ccgtgacagc gatgaagact ggtacctgcg cagtgaaaat
     2881 gcttatcgtg ctgaagtccc cctgtataca tccatg
//
LOCUS       DAZKGF010000320         2868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZKGF010000320 DAZKGF010000000
VERSION     DAZKGF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.321"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..354
                     /gene="radC"
                     /locus_tag="ACHOQV_005039"
     CDS             <1..354
                     /gene="radC"
                     /locus_tag="ACHOQV_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9633260.1"
                     /translation="AAREWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTE
                     VHPREVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLI
                     VGGSQVFSFAEHGLL"
     gene            423..644
                     /locus_tag="ACHOQV_005040"
     CDS             423..644
                     /locus_tag="ACHOQV_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9633261.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAGAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            720..1073
                     /locus_tag="ACHOQV_005041"
     CDS             720..1073
                     /locus_tag="ACHOQV_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9633262.1"
                     /translation="MSDTLPDGNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAG
                     IRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEADTLGSC
                     GYVYMAVYPTPETKK"
     gene            1163..1537
                     /locus_tag="ACHOQV_005042"
     CDS             1163..1537
                     /locus_tag="ACHOQV_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9633263.1"
                     /translation="MKTLPVLPGQSASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGTSSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            1534..2022
                     /locus_tag="ACHOQV_005043"
     CDS             1534..2022
                     /locus_tag="ACHOQV_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9633264.1"
                     /translation="MKLSLMLEADRINVQALNMGQIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGFPTF
                     DW"
     gene            2034..2231
                     /locus_tag="ACHOQV_005044"
     CDS             2034..2231
                     /locus_tag="ACHOQV_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9633265.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            2316..>2868
                     /locus_tag="ACHOQV_005045"
     CDS             2316..>2868
                     /locus_tag="ACHOQV_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9633266.1"
                     /translation="MSDITISRPELVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITCNLDREIWRDLMQCS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEENILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWD"
BASE COUNT          710 a          769 c          722 g          667 t
ORIGIN      
        1 gcggcacggg aatggctgat tctgaacatg gcgggactgg agcgtgaaga attccgggtg
       61 ctgtatctga acaaccagaa tcagttgatt gccggtgaaa ccctcttcac cggcaccatc
      121 aaccgcacgg aagtccatcc ccgggaagtg attaaacgcg ccctgtacca caatgccgct
      181 gccgtggtac tggcacacaa tcacccgtcc ggtgaagtca cacccagtaa ggcagaccgg
      241 cttattacgg aacgtctggt acaggcactg ggcctggtgg atatccgggt gccggaccat
      301 ctgatagtcg gtggcagcca ggttttctcc tttgccgaac acggtctgct ttaacccgtc
      361 accgtcacaa ccacatcata cctgttttca cttttatctt ctgtcttcag aggtatcaca
      421 tcatgaaaat catcacccgt ggtgaagcca tgcgtattca ccaacagcat ccggcctccc
      481 gtctttttcc gttctgcacc ggtaagtacc gctggcacgg cagtgccggg gcgtataccg
      541 gtcgtgaagt acaggatatt cccggtgtgc tggccgtgtt tgctgaacgc cgtaaggaca
      601 gttttggccc gtatgtccgg ctgatgagcg tcaccctgaa ctgagtcagg gcattctgat
      661 gagcagaatt atcgccacca ccggaccatt cttaaccgat tttctgtgag gattttatcg
      721 tgtcagacac acttcccgac ggcaatcacg accgcccctg gtgggggctg ccctgcaccg
      781 tgacgccctg tttcggcgcc cgtctggtgc aggagggtaa ccggttgcat taccttgccg
      841 accgcgctgg tatcagaggc ctgttcagcg atgcagatgc gtaccacctg gaccaggcct
      901 ttccgctgct gatgaaacaa ctggaactca tgctcaccag cggtgaactg aatccccgcc
      961 atcagcatac cgtcacgctg tatgccagag ggctgacctg cgaagccgac accctcggca
     1021 gttgtggcta cgtttatatg gctgtttatc cgacgcccga aacgaaaaag taactctcca
     1081 gaataaccct ctgctccggc ctggtgtttt caccacacca ctttcccatt tttcatcttt
     1141 gcatatcagg aaaatcttca gtatgaaaac attacctgta ttacccgggc agtcggccag
     1201 ttcacgccca tctcctgttg aaatctggca gacactgctc acccgactgc tggaccagca
     1261 ctatggcctt acgctgaatg acacaccgtt cgctgatgaa cgtgtgattg agcagcatat
     1321 tgaggcaggc atttcactgt gtgatgcggt gaattttctc gtggaaaaat acgcgctggt
     1381 gcgtaccgac cagccgggat tcagcgcagg aacttcgtcg cagttaatca acagcattga
     1441 tattctccgg gcacgccggg caacaggtct gatgacccgc gacaattaca gaacggtaaa
     1501 taacattacc ctgggtaagc atccggagaa acgatgaaac tttccctgat gctggaagcc
     1561 gacagaatta atgtgcaggc actgaacatg gggcaaattg tcgttgacgt cgatggtgtt
     1621 aatctcactg aactgattaa caaggtcgct gaaaacggtt attcactccg cgtggtggag
     1681 gaatccgacc aacagtcaac ctgcacacta ccaccgtttg caacccttgc cggcatacgc
     1741 tgcagtaccg cacatatcac ggaaaaggat aacgcctggc tgtactcgct gtcgcatcag
     1801 accagtgacg ttggtgaatc agaatggatt catttcacag gtagcggata tctgttacgt
     1861 accgatgcgt ggtcataccc ggttctgcgg cttaaacgcc tggggctgtc aaaaacgttc
     1921 cgccgtctgg ttgtcacact catccgacgt tatggcgtca gtctcattca tctggatgcc
     1981 agtgccggat gtctgccggg tttccccact ttcgactggt aaccagaaac aacatgaaat
     2041 cattaaccac ggaaacagcg ctggatattc tgattgcatg gctgcaggac aatatcgact
     2101 gcgaatcggg aattatcttt gacaacgatg aggataaaac ggattcggca gcactgctgc
     2161 cctgcattga acaggccagg gaagatatcc gtaccctgcg ccaactgcag tttctgcaac
     2221 agaaccggtg agtcttactc accagacttc attaccctga cgtcagcctg aacagggctg
     2281 gcgttttcgt ttataaaccc aaagggaaac cgataatgtc agacatcaca atctcccgtc
     2341 cggaactggt caccgggcat acggacgtta tctgctcaac cagtatccgc cacattctgg
     2401 ctgtacgaaa gagtacactg ctgcaaatcg acacacttat ccggcaactg gctgaaatat
     2461 cagcaatgac agaaagtatt ggcggtaaaa ccgcactgga ctgggcaatg aaacaggatt
     2521 ttcgctgcgg ttgctggctg atggaaaaac ctgaaacggc aatgaaagcc atcacctgca
     2581 atctcgatcg agaaatctgg cgtgacctga tgcaatgctc cgggatgctt tccttaatgg
     2641 atgcacaggc ccgtgataca tggtaccggt cactggagta cgataatttt ccggaaatca
     2701 gtgaagagaa tattctgagt acgtttgagc agctacacca gaataaggat gaagtgtttg
     2761 agcgaggagt gatcaacgtc ttcagagggt tgagctggaa ttacaaaacc aattgcccct
     2821 gtaaatttgg caataaaatt atcgtcaaca atctggtgag gtgggacc
//
LOCUS       DAZKGF010000321         2852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZKGF010000321 DAZKGF010000000
VERSION     DAZKGF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.322"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(223..588)
                     /gene="ylbG"
                     /locus_tag="ACHOQV_005046"
                     /pseudo
     CDS             complement(223..588)
                     /gene="ylbG"
                     /locus_tag="ACHOQV_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(704..1798)
                     /gene="mnmH"
                     /locus_tag="ACHOQV_005047"
     CDS             complement(704..1798)
                     /gene="mnmH"
                     /locus_tag="ACHOQV_005047"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFP9633267.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVKGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTR"
     gene            complement(1867..2793)
                     /gene="allS"
                     /locus_tag="ACHOQV_005048"
     CDS             complement(1867..2793)
                     /gene="allS"
                     /locus_tag="ACHOQV_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFP9633268.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAIEDIVTLFTQRRPEISGFLEIFGNPRS"
BASE COUNT          679 a          783 c          693 g          697 t
ORIGIN      
        1 tatctgaaaa taaataatcc ttccggatta aaataaattc tcgccgggaa agaaagagga
       61 aataaaccat tagcggaaaa ccagcggcac gccagggtga catgccccag tacggcggcc
      121 cgatagtcca gggttcagcg ggggtactga tagtcgcgcc gactgcatcg agaactccat
      181 acaacttttt acggaacgtc acctgataaa atttgttcag tatcgtcttt tgagattctc
      241 aaggttatgg agtaaccaga cagaacggac accactgtcg gagataaaaa ccccctgttt
      301 acgcagctcg ttgctggccc ggtgctgacc atgtgtcggg aaagcgacgg cgtaatcaac
      361 aacagcctgt tcaattgcct catcggtacg gttcttaagg ttaggagcgc ggcgactacg
      421 attaatctgc gcatccacac cgccttcagc ggccagttcg cggtaacgat aaaacgtatc
      481 acgcgaaacg cccatgattt tacaggcttt tgatacgttg ctgagttctt cagccagatt
      541 gagcaaaccg gctttgtgtt tgatgacggg attggcagta tgaagcatga gagttacctc
      601 ttgttttgga taaggattcg acactcatat caaaaccggt aactctcaac ctttcaaggc
      661 catgtgtcag atcaagtcgc gactaataca aatacgtccc tcattatcgc gtcttaaccc
      721 attccgccac ttccgcccac tcaccgcgaa agacaacttt ttccgctttt ttctcaagct
      781 gatagcgata catcgggtcg taatattctt caagtaacgg caccagccag gccagatgac
      841 cgtcggtgct gccggtggta agttgcgttg tcagcgccgc atccagcctt gcagccagtt
      901 cgttatagcg ctgtagcccc agccgacgct taatcgccga aagtccgtga tgcaggtatt
      961 cgcaatactc ctgccagccc tgttcgtcgc cgtacgcgtg ggtaaaatca tgatgcatac
     1021 gcaggaaata ttcttcgttc agccgctcaa gacggatctc aaacggatct tctactaccg
     1081 caatcgccgc ctgggtcatt cgctcgcgca ggcattccgg caggtgattc gaaccgatca
     1141 tccggctttc atcttccagc acccacaggc gcaaattctg acgggcgtcg gtttttagca
     1201 tttcggcagc cagcaggttt tcaaaactcg cctggctaag ttgtggttgt aaggtgcgac
     1261 caaacgccga accgcgatga cgcgccaacc cttccagatc aacaccgttc ggctgttgct
     1321 gcactaacag cgttttaccg ctgccggtac aaccgccaat cagcactatc ggtttttgtg
     1381 ccagttcaat agtcgcctga atcgcggtct ggcgcagtgc cttataaccg cctttcacca
     1441 gcggataatc aatccccgct gcatgcaacc agctttgcac aatatgtgag cgctgaccgc
     1501 cacgggcgca gcagagaata ccttgcggat tttgcaggca cgctgcccgc caggcgtcca
     1561 tacgctgctg acgaatttca cccgccacca gtttatgacc cagcgccagc gccgcgtctg
     1621 acccttgctg tttatagcag gtgccaacgg cggcgcgttc atcgttattc attaacggca
     1681 ggttgatagc ggcaggcatt gcgccttgct caaactcgat gggggcgcga acatcaataa
     1741 tgggcgtatc agcaatcagc agggcacgat agtcctgttc cgtgtgtctc tcttgcatag
     1801 taaaaagtga acctcaaatc agcttgcgcg ctattttacg cgccaacgcg caaggaaact
     1861 tgatttttaa ctgcgtgggt tgccgaaaat ttctaaaaat ccgctgattt ccggcctgcg
     1921 ctgggtaaac aaggtcacaa tatcttctat cgctttgccg ctgccaaatt tgcgccatgc
     1981 cagactcaat ggtgaaggag ggcgcatcgt tgggattacc cggctgacca gttgttgatt
     2041 atcgatcatt gactggcaaa gtgattttgg caaaaaacca atgccaacgc ccgccagatg
     2101 ggcggcgatt ttcgtttcca tatcgggaac aataatctct ttttgccccg gcaatcgcca
     2161 ggcgacgcgt ttggttagtg tgcgggcgct gtcttcaata ttaaccgccg gaaagcgccg
     2221 caactgtgct tctgttagcg gctcttcaac gttcgccagc ggatgatccg ccgccatgac
     2281 aaagcgccat tgcaccgatc ctaagggatc aagactaaag gtatttgcca gcgcctcggt
     2341 tcccgtgacg ccgatagcca gtgaaaaacc ttcgtataat agcgaatccc agacgcccat
     2401 atagatttgt cgggagatgt gaaactgggt aaaggggtaa cgttcattca gccacgccag
     2461 caactgggcg acggcctggg ggttatagag caggttgttg atgacaatat tcacctggcg
     2521 ttccacgcca tcattcacct gttgcagctc gctgggcata ctttccagcc agctcagcca
     2581 gtctctggcc tgacaaagta gatgctcgcc agccgccgtc aacgtcacgc tacgagtcgt
     2641 acggaaaaac agcgctactc cggtattctc ttccagaagt ttaatgcgat aactgatcgt
     2701 cgccgtggtt ttacataatc gttctgccgc ttttgaaaaa cttcctgttt cagcaaccgc
     2761 aatgaaagtc cgcaaggttt ctggatcgaa catcttcagg tatccccttt taaatccgca
     2821 agttgcgtga ttttcttatc ctctgattta tc
//
LOCUS       DAZKGF010000322         2845 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZKGF010000322 DAZKGF010000000
VERSION     DAZKGF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.323"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..840
                     /locus_tag="ACHOQV_005049"
     CDS             <1..840
                     /locus_tag="ACHOQV_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFP9633269.1"
                     /translation="LENAAERAQALKADIDSQIQMFEQRLKPLNEAVLFSAPEGIALT
                     SGEDVQLAAGQNLAVNAGGDISTGTMGNLTALAGERLGLFARSGQLSVKSGEGPVEVQ
                     AQNGSMRLFAEKKLTLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTTVSYLRKV
                     KRTIAAGAASQQPEMPWLADPVKPSASALMQYLYHDDVPVVGAPFKATLADGTVREGN
                     LDGAGYLHLEDVPPGRVLVELGPDTRDYLRKNKTDNRLFVGTSLSEADIDSLIAKYSE
                     GVE"
     gene            837..2381
                     /locus_tag="ACHOQV_005050"
     CDS             837..2381
                     /locus_tag="ACHOQV_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633270.1"
                     /translation="MSGVNVAWFKETSLETGQWIWGTLQGAFNEKQTIGQIIVDAVIG
                     MIPLVGDVTAVRDLIAVSIGLAKEPKKRESVTEWVFLVILLFALIPVIGGVIKGVGRL
                     ALRVTTKVAENVETLGAVIKFLNRIGEGDAVKWLNKLNVVEYQAQLIEKMGDFCNTMI
                     TAINKILKARVGRMLPEKWQADLLLVRDGFAALQRLASRMIPQALKELDAKLRVLQAL
                     VYKGEIHTITTGMGRAEVRREAEAYLIERPLCEAPKQGTRYPSVHAEVIHEDRLRLKY
                     SREGYPDLLGYKGKSPAFGDEQVFTNIASFSGEITAKSASEMGGSTIFRAFGNPTELA
                     PNGSWAAGGFWGYQKVPGSAEEWRQLSAVLDEWNGNGFLVVLHLPEDLATRMPGSNAW
                     HGKIAEQFGQKRPDQYLQGGGEQVIINLDETLKTELNRIGEQVKASGQAVSIEINGVN
                     AEFYPTGWKEVEGVHGYSKPEGIEPPYVTYTRELHDDELRSKVTERTILNAARIEYQT
                     TTGSEQ"
     gene            2378..>2845
                     /locus_tag="ACHOQV_005051"
     CDS             2378..>2845
                     /locus_tag="ACHOQV_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633271.1"
                     /translation="MITIEKTKYPMSDEQVRRKLFWVLQRLSSYSLWQRKRDAWAYFT
                     QQYEQALKNWPEEITKGFDPEAIIWAYDALRLYDEGLSELAVGNRQVWQRLTGEFHQL
                     ARPVDLIESYFYLPCHERGVQQEPYPVEVEKLNKLKIAAKIHGDYLMYPPHNNV"
BASE COUNT          751 a          572 c          892 g          630 t
ORIGIN      
        1 ctggaaaatg ccgcagaacg ggcacaggcg ctgaaggctg atattgacag tcagatacag
       61 atgtttgagc agcggctgaa accgctcaat gaagccgtgc ttttctcggc gccggaaggc
      121 attgccctga ccagcggtga agatgtgcag ctggctgctg gtcagaacct ggctgtcaac
      181 gccggaggtg atatcagtac cggaacaatg ggcaacctga ccgcgctggc cggagagagg
      241 ctggggcttt ttgccaggag cggacaactg agcgtcaaat ccggggaggg gccagtagag
      301 gtacaggcgc aaaacggcag catgcggttg tttgctgaga agaagctgac gctgagttcg
      361 gcaagtgata tctcctttgc cgggaaaaaa cgtatcacgc tgattggcgg ggggagttac
      421 ctgcggctgg aggcggggaa aatagagtac ggtacgacgg tgtcgtatct gcgcaaagtt
      481 aaacggacta tagcggcggg ggctgcgtca cagcagccgg aaatgccttg gcttgccgac
      541 ccggttaaac cctctgccag tgcgctgatg cagtatctgt atcacgacga tgtcccggta
      601 gtgggcgcgc ctttcaaagc gactcttgct gatggcactg tacgggaagg taaccttgac
      661 ggtgcgggat atctgcatct ggaagacgtc ccgccaggga gagtgctggt ggagctcggg
      721 ccggatacca gagattattt acgtaaaaat aagaccgata atcggttgtt tgttgggaca
      781 tctctcagcg aggctgacat tgactcactg atagcgaagt acagcgaagg tgtggaatga
      841 gtggagtaaa cgttgcctgg tttaaggaaa ccagcctgga aaccggacag tggatctggg
      901 ggacgcttca gggggcattt aatgaaaaac agaccattgg gcagatcatc gttgatgcgg
      961 tgatcggcat gatcccgctg gtcggcgatg taacggcagt gcgagatctg atagcggttt
     1021 cgataggact ggcaaaagaa ccgaagaaac gggaaagtgt gactgaatgg gtgtttctgg
     1081 tgatcctgct gtttgcgcta atcccggtaa ttggtggcgt gataaaaggt gtcggacgtc
     1141 tggccctgcg tgtgacaaca aaagtggcag agaatgtgga aacccttggc gctgtgatta
     1201 agtttctgaa ccgtatcggc gaaggtgatg cggtgaagtg gctgaataag ctgaatgtcg
     1261 ttgagtatca ggcacagttg attgaaaaga tgggggattt ttgcaacacg atgattaccg
     1321 ccatcaataa aatcctgaaa gcccgggtcg ggcggatgtt gccggagaaa tggcaggcgg
     1381 atttactctt ggttcgggat ggttttgcgg cactgcaacg gctggcgtcg agaatgatcc
     1441 cgcaggcgtt gaaagagctg gatgcgaaac tgcgggtgtt gcaggcgctg gtttataaag
     1501 gtgaaataca tacaatcacg acggggatgg gacgtgctga agtgcgccgt gaggctgaag
     1561 catatcttat tgagcgtccg ctgtgtgaag cgccgaagca ggggacgcgg tatccgagtg
     1621 ttcatgcgga ggtaattcac gaagacagat tgcggcttaa atattccagg gagggatatc
     1681 cagatttgtt gggatataaa ggaaaaagtc cggcatttgg tgatgagcaa gtatttacaa
     1741 atattgcctc ttttagcggc gagattaccg caaaaagtgc aagtgagatg ggcggtagta
     1801 cgatttttcg cgcttttggt aatccaacag aattagcacc caacggttct tgggcggctg
     1861 gcggattctg ggggtatcaa aaagtgccgg gcagtgctga agaatggcgt cagctttcag
     1921 ctgtactgga tgagtggaat ggtaacggtt ttctggtggt gctgcatttg ccagaagacc
     1981 tggcaactcg tatgccgggt agcaatgctt ggcacgggaa aattgctgaa cagttcggac
     2041 agaagagacc tgaccagtat ctgcaaggtg gtggcgaaca ggtaattatt aatctggatg
     2101 aaacgttgaa aacagagttg aaccgcatcg gggagcaggt aaaagccagc ggtcaggctg
     2161 tcagtattga aattaatggc gtaaatgccg agttttatcc gacgggctgg aaagaggtgg
     2221 aaggcgtgca tggttacagt aagccggaag ggatagaacc gccgtatgtg acgtataccc
     2281 gtgaactgca tgacgatgaa ctgcgcagca aagtgacaga gcgaacgata cttaatgcgg
     2341 cccgcattga gtaccagacc acgacaggaa gcgaacaatg attaccattg aaaaaacaaa
     2401 atacccgatg agcgatgagc aggtacgtcg caaattattc tgggtgttac agcggctgag
     2461 cagctatagc ctttggcagc gcaaacggga tgcctgggcc tattttaccc agcagtatga
     2521 acaggcgctg aaaaactggc cggaagagat aacgaagggg tttgatcccg aggcgataat
     2581 ctgggcatat gatgctttgc gtctttacga tgaagggcta tcagaattgg cagtgggaaa
     2641 tcggcaggta tggcagcgtt taacgggaga atttcatcaa cttgctcgcc cagtggattt
     2701 aattgaatct tatttttatc tcccctgcca tgaacgaggt gtacagcaag aaccataccc
     2761 ggtagaagta gaaaagctta ataagctgaa gattgcagca aaaatacatg gtgactatct
     2821 aatgtatcca ccacataaca atgta
//
LOCUS       DAZKGF010000323         2835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZKGF010000323 DAZKGF010000000
VERSION     DAZKGF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.324"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(196..927)
                     /gene="dnaQ"
                     /locus_tag="ACHOQV_005052"
     CDS             complement(196..927)
                     /gene="dnaQ"
                     /locus_tag="ACHOQV_005052"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFP9633272.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQDETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            992..1459
                     /gene="rnhA"
                     /locus_tag="ACHOQV_005053"
     CDS             992..1459
                     /gene="rnhA"
                     /locus_tag="ACHOQV_005053"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFP9633273.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1456..2178)
                     /locus_tag="ACHOQV_005054"
     CDS             complement(1456..2178)
                     /locus_tag="ACHOQV_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9633274.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGLPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2212..>2835
                     /gene="gloB"
                     /locus_tag="ACHOQV_005055"
     CDS             2212..>2835
                     /gene="gloB"
                     /locus_tag="ACHOQV_005055"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFP9633275.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILK"
BASE COUNT          745 a          701 c          674 g          715 t
ORIGIN      
        1 ggactcgaac ccgcgacccc ctgcgtgaca ggcaggtatt ctaaccgact gaactaccgc
       61 tccgcgtttt gttccgttgg gaacgaggcg aatattacgg attgcctcac ccttcgtcaa
      121 cggtttttct catcttttga atcgtttgct gcaaaaatcg cccaagtcgc tatttttagc
      181 gccttttaca ggtatttatg cccgccagag gcagcttccc cccttcttct gcaccagatc
      241 gagacgggct tcatgtgctg caagctcttc atctgtcgca aaaacaacgc gtaacttact
      301 tgcctgacgc acaatgcgct gaattgttgt ttcatcttgt tgctgttgtg tctctccttc
      361 catcgcaaaa gccatcgacg tttgcccacc agtcatcgcc agataaactt ccgcaaggat
      421 ctgggcatcg agtaatgccc cgtgcagcgt tcgtttactg ttatctattt cgtagcgggc
      481 acataacgca tcgaggctgt tgcgcttacc gggaaacatt ttcctcgcca ccgcaaggct
      541 atcggtgacc ttacagaagg tattggtctt cggaatatcg cgcttaagca acgaaaactc
      601 gtagtccata aagccgatat cgaacgctgc gttatggatc accagctccg cgccgcggat
      661 atagtccatg aactcatcgg ctacctctgc aaacgtgggc ttatcgagca aaaattcatc
      721 ggcaatacca tgtacgccaa aggcttccgg atccaccagc cgatcgggtt tgagataaac
      781 atggaagtta ttgcccgtca ggcgacggtt caccacttca acggcaccaa tctcaatgat
      841 cttgtggcct tcatagtgcg caccaatctg gttcataccg gtagtttcgg tatcgagaac
      901 gatctggcgt gtaattgcag tgctcatagc ggtcatttat gtcagacttg tcgttttaca
      961 gttcgattca attacaggaa gtctaccaga gatgcttaaa caggtagaaa ttttcaccga
     1021 tggttcgtgt ctgggtaatc ctggacctgg gggttacggc gctattttac gctatcgcgg
     1081 acgcgagaaa acctttagcg caggctacac ccgcaccacc aacaaccgta tggagttgat
     1141 ggccgctatt gtcgcgctgg aggcgttaaa agaacattgc gaagtcattt tgagtaccga
     1201 cagccagtat gtccgccagg gtatcaccca gtggatccat aactggaaaa aacgtggctg
     1261 gaaaacagca gacaaaaaac cagtaaaaaa tgtcgatctc tggcaacgtc ttgatgctgc
     1321 gctggggcag catcaaatca aatgggaatg ggttaaaggc catgccggac acccggaaaa
     1381 cgaacgttgc gatgaactgg ctcgtgccgc agcgatgaat cccacactgg aagatacggg
     1441 ctatcaagtc gaagtttaag cctgtggttt acgacattgc cgggtggctc caaccgcctg
     1501 gcgaattcgt ggcttgtttt tactctgttt catcggattt agcgttaaag gaatggtccg
     1561 tttccgggca acaataagtt gtaagcaacc aagcgcagga atatgcgcat tcaatagttt
     1621 tcctccgtgt ttgttccacg ggagaacgtg gaaacggctg gcgtgtagca cttcaaaatt
     1681 caacaaagag agccagtcca gctgccgcat cagagtaaac atccggctgt tatagggcga
     1741 ggttttgcgc aataccggca caagtttgcg taatcccatt aaactgatgg gattgaagcc
     1801 actaatcacc agccagccat catcaatcaa tacccgatcg gcttcacgca ataaacgatg
     1861 cggatcggtg caccacggca atgtatgtgc cagtagacaa acatcaacgg atttatcggc
     1921 aaaaggaaga tgaagtgggt ccgcctgcac ctggacgggc agtccttgcg cagaaacatt
     1981 cacttgatga gaaaccgcgc acgcttcgca attgatttct gcgcttaaat tgccaatctt
     2041 aagcagatga aaaccataca ttttagtgaa ccacgggttg agctggcgct ccagcgcctt
     2101 gcgataaagc tctccccagg gcaaatcgcc ccagcaatca ggagccacga cagtttgagg
     2161 gactcttgcc ggtttcatca caaccttccg tttcacactg agaggtaatc tatgaatctt
     2221 aacagtattc ccgcctttga tgacaattac atctgggttt tgaatgatga agcaggtcgc
     2281 tgcctgattg tcgatcccgg agacgcagag ccagtattaa acgccattgc cgccaataac
     2341 tggcaaccgg aggccatatt tctcacccac catcaccacg atcacgttgg cggcgtaaaa
     2401 gaactggtga aaaagtttcc gcaaattgtg gtgtatggtc cacaagagac acaagataag
     2461 ggaacaacac aggtagtcaa agatggcgaa actgccttcg ttttggggca tgaatttagt
     2521 gtaattgcca cgccgggtca cactttagga catatctgtt acttcagtaa accttatcta
     2581 ttttgcggcg acacgctgtt ttctggtggg tgtggtcggt tgtttgaagg gacagcatca
     2641 caaatgtatc aatcacttaa aaagttaagt gcgttacctg acgatacatt ggtatgttgt
     2701 gctcatgaat ataccttatc aaatatgaag tttgctttga gtattcttcc gcacgatttg
     2761 tccataaatg attattatcg taaagttaag gagttacggg caaaaaatca aataacacta
     2821 cccgtaattc tgaaa
//
LOCUS       DAZKGF010000324         2821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZKGF010000324 DAZKGF010000000
VERSION     DAZKGF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.325"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2691)
                     /gene="chiA"
                     /locus_tag="ACHOQV_005056"
     CDS             complement(<1..2691)
                     /gene="chiA"
                     /locus_tag="ACHOQV_005056"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HFP9633276.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEVWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNPWRYVRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEISQYGNPGSCSVKPDNNSGAVTPVDPTPETPVTPVTPTPDNNEPSTPADS
                     SNDYSLQAWSGQEGSEIYHVIFNGNVYQNAWWVGSEDCPRGTSAENSNNPWRLVRTAT
                     AAEMSQYGNPTTCEIDNGGVIVADGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVP
                     GGDNPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNS
                     RPWKPLGKAQSYSNEELNNAPQFNPETLYASDTLIRFNGANYISQSKVQKVSPSDSNP
                     WRVFVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVV
                     SKDANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDL
                     MQHYYDIVDNLNLRVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTL
                     PILPTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSA
                     IANLHSQLKGLHPNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLG
                     MVGIWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPF"
BASE COUNT          684 a          736 c          657 g          744 t
ORIGIN      
        1 aaacggcgcg aagatttcgc taaatgcgta cttcggtgcc tgttctttag tcaggccgtg
       61 gaattccgga gacaggttag tgccgcctgg caggtcgcgc gcgattgacc agataccaac
      121 cataccgaga ttacgcttct gcgcatcctg catgaccaga cgagcatcag agagatagaa
      181 cacctcgccc tgaacgtcgt tcacgccaac catcggcgtg gtacccatca tagcgtcaat
      241 ttctgcatcg ctcttattgg gatggaggcc tttcaattgt gaatgcaggt tggcaatcgc
      301 agacgttgca cacttaccgt gaatgttctg gccttcggta tttgcagact gacaaatcgc
      361 gttaccgtag tccattgtca tcacgttcac acccgccagc tcaacacctt tcgctttggc
      421 atcgctcagg acattcatcc cttccggcgt caggccagtc ggcagaattg gcaaggtgta
      481 ccagatagca atatctttgc cctctgactt ccatttatcc tgcactttct tcacagcaag
      541 gttacgacgt tcaatagatg cctgatccgc aacccaggtg ccttcgatat cgaagtccag
      601 gactctgagg ttcaggttat caacgatgtc ataataatgc tgcatcagat cgtctacgtt
      661 cttacaggaa gcagccagcg gagcgttgtt agcaccaccg atagacagca tcacatcgcc
      721 gccagcttca cgcagagctt tgattttgct gtactgagcg taattctgca taccgtaagc
      781 ggtaccccat gtcggcagac aggtgttcgc atctttactc accacaaacg ccagcgtgaa
      841 gtggttgacg ttatgattct tagccagcgc agccagatcc gggatcgtat tcagcgtaaa
      901 gtcaacatac ggtgcataaa cgtgtttcgg ccacgctttc ttcggcgtac ctacacgttc
      961 tttggttccg gtccagtcaa caaaaacacg ccacgggttg ctgtcagaag gagaaacttt
     1021 ctgcacttta ctctgagaaa tgtagttcgc accgttaaag cgaatcagcg tatcgctggc
     1081 ataaagcgtt tctggattaa actgcggcgc attattcagc tcttcgttgc tatagctctg
     1141 agccttacct aacggcttcc acgggcggct attgctaccg gtggcgtttt ggttcgcaac
     1201 cagagctggg ttgtcgcttt gcgtccagaa cagcgcttca taagcctgcc catcaacaac
     1261 aacgcgatca cctttcacgt acacggtgga agcggaccat gctttcgccg gttcgtattt
     1321 cttccacggg ttatcgccgc ccgggacaaa gccccatgcg tcttgatcga cagaggtttt
     1381 ataatgtgca tcgttataat ctacgatgct gttcgcgctg tacgctttgc tggcctggaa
     1441 accatccgca acaatgacgc cgccattatc aatttcacag gtagttgggt taccgtactg
     1501 actcatttcc gcagcggtag ctgtacgcac gagacgccat gggttattgg agttttctgc
     1561 actagtgcca cgtgggcaat cttcagaacc aacccaccag gcattctgat aaacattacc
     1621 attgaagata acatggtaaa tttcgctacc ttcctggcca ctccacgcct gcagtgagta
     1681 atcgttactg ctatccgctg gtgttgatgg ctcgttatta tccggagtag gagtaactgg
     1741 agttaccggt gtttccggag ttggatcaac cggagtcaca gcaccgctat tattatccgg
     1801 cttaacggaa caggaacccg gattaccata ctggctgatt tctgttgccg ttgccgcgcg
     1861 aacataacgc catgggttgc tggcatcgtt ttctttcgca tcgcctgggc aatttgccgc
     1921 gcctacccac caggcgttct tataaatgcc gccgttgtag ataacgtacc atgtagaaga
     1981 accttcctga cctttccagg ctacgacagc atagttgtcg tttgacttat cagctggagc
     2041 tggctgaaca tcaggagccg gtttaacgtc cggtgccggt ttcacatccg gcgcaggctg
     2101 tggtgctgaa gtacaggact gtggattact ggtttctgag atttccgttg ccgtagcggc
     2161 acgaacataa cgccatgggt tgctggcatc attgcctttc gcatcacccg ggcaattaga
     2221 agaggctacc caccaggcat ttttatatac cgcaccatta aagacgacat accaggtctg
     2281 accgccctgc tgtttattcc aggcaactac agaagaatta gacggaacag tgccctgtgc
     2341 tggtgtagcc gaaccgccat tagccggcgt attggatgca ggcgtatttg aatttgaaga
     2401 agatgagctg ccgctctttt cgcaggaaag tgtattgcca aactgactaa tttcagcagc
     2461 tgtcgcggta cgctttaaac gccatgggtt agttgcatca tttgcttttg cttttcccgg
     2521 gcaatttgta gaagaaaccc accaggcatt ttcataaatt ttgccatcga aaataacctg
     2581 atatttatta ccaccttgtt ggttattcca tacttccatt gccaatgctg gcagagcgga
     2641 acacaccagc cccataccaa tcatagattt agtaaatata tttaatttca ttataatccc
     2701 ttgtgacgta aaaactgcaa aacaaaagtc ctacgcagga attatttacg ttttacgaga
     2761 acccttcaat atgaattaaa ttacggcatt aaaaataaga aaaaagcctg acaaaagaag
     2821 c
//
LOCUS       DAZKGF010000325         2819 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZKGF010000325 DAZKGF010000000
VERSION     DAZKGF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.326"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(13..468)
                     /locus_tag="ACHOQV_005057"
     CDS             complement(13..468)
                     /locus_tag="ACHOQV_005057"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFP9633277.1"
                     /translation="MPDMRLFKSKNTKLELRIRKPLFARGFRYRLHNSGLPGRPDMYF
                     PKYNAVIFIHGCFWHGHDNGCPISHTPKNNESYWQNKINKNQERDVKNQYVLASKNIR
                     VLIIWECAIKLKSIDFVDFIDYIDEWLRGGANSTQMSAKEPMPTFEFPK"
     gene            694..1833
                     /locus_tag="ACHOQV_005058"
     CDS             694..1833
                     /locus_tag="ACHOQV_005058"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFP9633278.1"
                     /translation="MSTMKPAIFSFFSGSGFLDLGFENAGYEVVFVNEFHPPFMDAYK
                     HSRKKLGKQPPRFGYAQESIETIEHSVIKHSMKTLKGENRLVGFIGGPPCPDFSVAGK
                     NKGKDGENGKLSQSYIDLIIEMMPDFFLFENVKGLYRTLKHREFFEQIKNELASVGYI
                     LHERLINSIEYGAPQDRERIILVGFLDKSLAKNFEWSGQIYPNKEAFQFPWPTTNTYE
                     EDSSMECPQGIPEELTIEYWFEKNDVTNHPNAKHVFTARAGLARFQSVEEGDDSKKSY
                     KRLHRWRYSPTAAYGNNEVHLHPYKPRRITVAEALAIQSLPKNFELPGDMTLSNMFKT
                     VGNGVPYIAALGIAQSILQHLGYSNEDNSIESGSGDFELTQKYAV"
     gene            1769..>2819
                     /locus_tag="ACHOQV_005059"
     CDS             1769..>2819
                     /locus_tag="ACHOQV_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9633279.1"
                     /translation="MRITASNLVQAISSLPKNTLFNYINDRNSGKIEIVRIQHPEGPI
                     EIKRFDPKKGQTQATAKTESISTQMLWRLANALEENRPVNVERVFGASYNTRSVLESL
                     LAHTPEFYWCRPARLEIMNAQKSIKPGHKHLIFLPDMPHTNGLLIEHQTNIVVSEMSF
                     DVVHQSVDIETIKPTKGMTIEEKRRHAQIQIALVKIGYCLGLDTWVAANDRSLQYNGK
                     AIAQMDGVISSLSDEQVLQSYNDAIKDARLIDCIWFREGRMMPAVMEIEHSTGIKSGL
                     TRMKQFYDHAPRLQDIRWTIVAPDEYRAKVIEFANMPQFIELKAKFFPYSSVEELYSL
                     CDRRKPKGINDLFLDS"
BASE COUNT          935 a          535 c          538 g          811 t
ORIGIN      
        1 tgtcataata attcatttcg gaaactcaaa agtcggcatt ggttcctttg cgctcatctg
       61 tgttgaattt gccccacctc taagccattc atcgatgtaa tcaataaaat cgacaaaatc
      121 aatactttta agttttattg cacactccca tataatcaga acgcggatgt ttttagatgc
      181 aagaacatat tgatttttga catctctttc ttgattttta ttgattttat tttgccagta
      241 cgattcatta tttttaggag tgtgagatat agggcagccg ttatcatgcc catgccaaaa
      301 acagccatgg ataaaaatga ctgcattgta tttagggaaa tacatatctg gtctaccagg
      361 caaaccacta ttatgcaatc gataacgaaa ccctctggca aacaacggct tcctgattct
      421 taactcaagt ttggtgtttt ttgatttaaa taacctcata tcaggcatat aagttccttc
      481 ggtttctaaa tctataatag cagcttatac tattaatcaa tatattaaca cctaacttta
      541 tagcaatgaa tactatctaa tctgaagtag tgccaggtct tatttgctaa tatattaaaa
      601 agtaaattga taattaacaa gttatcatgt aaaatagtag gatcgtacag cgagatattt
      661 agtaaaaatt taagctaact tgataggtat aaaatgtcca caatgaaacc cgctatattt
      721 tcattctttt caggatcagg ttttcttgac ctaggcttcg aaaatgccgg gtatgaggtg
      781 gttttcgtca atgaattcca tcctcccttc atggacgcgt ataaacactc aagaaaaaaa
      841 cttggcaaac agccgccacg ctttgggtat gcccaagaaa gcattgaaac tattgagcat
      901 tcagtgatta agcatagcat gaaaacactg aaaggtgaaa accgcctagt gggcttcatt
      961 ggcggaccac catgccctga cttttctgtt gctggcaaaa acaaaggcaa agatggtgaa
     1021 aatggcaaat tatcacaaag ttacattgac ctgataattg aaatgatgcc tgatttcttc
     1081 ctttttgaaa acgttaaagg cttataccgg actcttaaac atagagaatt tttcgaacaa
     1141 attaagaatg aactagcttc agtaggatat atcctgcatg aaagattgat taattcaatt
     1201 gaatacggtg cgcctcaaga tcgagaaagg attattttag ttggttttct tgataagtct
     1261 ttagccaaaa acttcgaatg gagtggccaa atatacccca ataaggaagc tttccagttc
     1321 ccttggccaa caacaaacac atacgaagaa gactcaagta tggaatgccc gcaaggtatt
     1381 ccagaagaac ttactataga atactggttc gagaaaaacg atgttaccaa tcatccaaac
     1441 gctaaacatg tatttacggc ccgagccggt ttagcaagat tccagtcagt agaagaaggc
     1501 gacgatagta aaaaatcata taaaaggctt catcgctgga gatactctcc aacagcagca
     1561 tatggtaata atgaagttca ccttcaccca tacaaacctc gtcgtattac ggtcgctgaa
     1621 gcattggcaa tccagtcttt gccaaaaaat tttgagctac caggggatat gactttgtcg
     1681 aatatgttta aaacagtcgg aaacggagtt ccctatatcg ccgcgctcgg tattgcccaa
     1741 tcaattttgc aacatttagg atacagtaat gaggataaca gcatcgaatc tggttcaggc
     1801 gatttcgagc ttacccaaaa atacgctgtt taactatata aacgatagga atagcgggaa
     1861 gattgagatt gtacgtatcc aacatcccga aggtcctatc gaaataaaga gatttgatcc
     1921 taagaaaggt caaactcaag caacagctaa aacagagagt atttcgactc aaatgctttg
     1981 gcgacttgca aatgcactcg aagaaaacag acctgtcaac gttgaaaggg ttttcggtgc
     2041 cagttacaac acccgctcgg tattggagag cttgctcgca catactccag agttctattg
     2101 gtgtaggcca gccagactgg aaatcatgaa tgcacaaaag tctataaaac caggacataa
     2161 gcatctgatt tttctgccag atatgccaca tactaatggt ttgttaattg agcaccagac
     2221 taatattgtt gtctctgaaa tgagcttcga tgttgttcac caaagcgttg atattgagac
     2281 aattaaaccg actaaaggca tgactattga agaaaaaaga cgtcatgctc agatacaaat
     2341 tgccttagta aaaataggtt attgtttagg tttagatact tgggtagcag ccaatgatcg
     2401 tagcttacaa tataatggta aagctatagc gcaaatggac ggtgtaataa gttcattatc
     2461 tgatgagcaa gtactccaat cgtataatga tgcgattaaa gatgctcgtc ttatagactg
     2521 catttggttt agggaaggcc gcatgatgcc agcagtaatg gagattgaac acagtactgg
     2581 tataaaaagt ggtctgactc gcatgaaaca attctatgat catgcaccga gattacaaga
     2641 tatccgttgg actatcgttg ctcctgatga atatcgagca aaggtgatag aatttgcaaa
     2701 catgccccaa ttcattgaat taaaagctaa attcttccct tactcttcag ttgaagaatt
     2761 atactctcta tgcgaccgaa gaaaacctaa aggaataaat gatttatttc tagattctt
//
LOCUS       DAZKGF010000326         2818 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZKGF010000326 DAZKGF010000000
VERSION     DAZKGF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.327"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            185..910
                     /locus_tag="ACHOQV_005060"
     CDS             185..910
                     /locus_tag="ACHOQV_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9633280.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            868..1545
                     /gene="bglJ"
                     /locus_tag="ACHOQV_005061"
     CDS             868..1545
                     /gene="bglJ"
                     /locus_tag="ACHOQV_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFP9633281.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1582..2370)
                     /gene="fhuF"
                     /locus_tag="ACHOQV_005062"
     CDS             complement(1582..2370)
                     /gene="fhuF"
                     /locus_tag="ACHOQV_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFP9633282.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNTLSSQLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     MLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2511..2747
                     /locus_tag="ACHOQV_005063"
     CDS             2511..2747
                     /locus_tag="ACHOQV_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFP9633283.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIIILMGLVLTP
                     AMLYHKQLRHYVLLPSCLALIAGIMLMIMNLKQG"
BASE COUNT          746 a          607 c          710 g          755 t
ORIGIN      
        1 ttatcatttg atgctaacct tctgacacat gcagtggagt tgttgtgcag caggagtatg
       61 ctgatatgaa agtgaatgct aaggataatt tattcgctta atctattaat ttgctggaaa
      121 catttaagga atgaatatcc tgaataaacc atcatgattt ttagacagtg gatgtggagg
      181 aaatatgttg ccaggatgct gcaaaaatgg aattgttatc agtaaaatac ctgttatgca
      241 agcagggtta aaagaggtca tgaggactca cttccctgaa tatgaaataa tatccagcgc
      301 ctctgcggag gaccttacct tattacaatt acgtcgttcc ggattagtca ttgctgattt
      361 agccggtgaa agtgaagatc cacgttctgt ttgtgaacat tattattctt taatctcaca
      421 atatcgggaa attcactggg ttttcatggt gtcacgctcc tggtattccc aggcagtaga
      481 actgcttatg tgccctacgg cgacgttatt gtctgatgtt gaacccattg agaatctggt
      541 caagaccgta cgttccggca atacgcacgc agagcgtatc agcgccatgc tgacctctcc
      601 ggcaatgact gaaactcatg attttagcta tcgctccgtc attctcactc tttcagagcg
      661 caaggtactg cggctattag gtaaaggatg gggcatcaac cagatagctt cattgcttaa
      721 gaaaagtaat aaaactatca gcgcccaaaa aaacagtgcg atgcgtcgac tggcaattca
      781 cagcaacgct gaaatgtatg catggataaa tagcgcgcag ggtgcaagag aacttaactt
      841 gccttctgtt tatggagatg ccgcagaatg gaacacagcc gaattaagaa gagaaatgtc
      901 gcactcatag aaaaatgcgt catgagcagt atcggtattg agagtttatt cagaaagttt
      961 gcgggtaacc cttataagct ccatacctat accagtcagg agtcatttca ggatgccatg
     1021 tcgcggatct cgtttgcggc ggtcattttt tctttttctg ccatgagaag tgagcgcaga
     1081 gagggattat cttgcctgac tgaactggcg attaagtttc cgcgtacccg gcgtttagtt
     1141 attgcggatg atgatattga agctcggctg attggttcat tgtcgccatc accgttggac
     1201 ggtgtattaa gtaaagcgtc aacgctggag atttttcatc aggaactctt tttgtcatta
     1261 aatggtgtac gtcaggcgac cgaccgactg aacaatcagt ggtacattaa tcaaagccga
     1321 acgctaagcc cgacggagag agaaatattg cgctttatgt cgcgtggcta ctcaatgaca
     1381 caaattgccg agcagcttaa acgcaatatc aaaacgatcc gtgcacataa atttaatgtg
     1441 atgtcgaaac tgggcgtcag ttctgatgca gggttgttgg aggccgcaga tattctgtta
     1501 tgtatgcggc attgcgaagc aagtaatgtg ttgcatccct attaatccac atgatgccgg
     1561 gtttacttcc ccggcagtgc ttcatttcag cgtacaatcg ccacattgct gcacatccgg
     1621 taagcgataa cgctggcagc aagtgcggcg caccagcagg ccgtcgcgca gtaccacggt
     1681 acgccagagt ggattatctt caccgttcgt gagcattttc tcaaaaaaga gggcatggcg
     1741 cagcgattca accgtcgcct cgccaagcag ctgtttcatc tcagtcagat accagttgat
     1801 caaataaccg gtattgctcc agataagttt gccgttgatc tctcccgtcg cttctaatgc
     1861 ctgcactacc ggaacaagcg cctggctgat taacgtttcc attcgctgct gcggcgaatg
     1921 tagtgttgcg tttttatctt cacacacatc gacccagaaa caggcggcgc gtccggtttc
     1981 gtgaaactca acgtggaaat gttccggcga cacatctaat gctttttcct gcgtcagcag
     2041 cgccaacatt aatggtggca ccatcaggcc gatataccat tgtgcccata gtgagatcag
     2101 cggtttgttc tcgcggatca tcaagggttg gttgcggtag atatgatcgg aatagaccgc
     2161 cagttgagaa cttagcgtat tcggtgatga ccattgcgcc agcgtcatgg cacggagcgg
     2221 tgcgggttca tccagacgga taaactccag caaatgttca cgatgttcgg cgatcgtcgc
     2281 ccgtacggct tgtgcaagcc cagcatcctg cggctggaga tgcgttcgcc agataacatc
     2341 ttcatagagc ggtgcggaac gataggccat aatcgtgata gtaatctaaa tgataatgat
     2401 tgctaatcct agcgataggt ttacccgata gcaagggatt tctctggctt gcaaatgata
     2461 aaaattatca tatgatattg gttatcatta tcaatgaaag agatgaaacc atgttgcaac
     2521 gtacgctggg cagtggctgg ggagtgttgc tgccgggatt gctgattgca gggctgatgt
     2581 atgcggattt atcgccggat caatggcgga ttatcattct gatgggatta gtattgacgc
     2641 cggcgatgct gtatcacaaa cagttgcggc attacgtttt gctgccatcg tgcctggcgc
     2701 ttattgctgg catcatgctg atgataatga atttgaagca gggatgataa aacagggaag
     2761 aaacaagaaa ggaagtaaag ataattggtg cgaggggggg gacttgaacc cccacgtc
//
LOCUS       DAZKGF010000327         2783 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZKGF010000327 DAZKGF010000000
VERSION     DAZKGF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.328"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(736..2673)
                     /gene="iolC"
                     /locus_tag="ACHOQV_005064"
     CDS             complement(736..2673)
                     /gene="iolC"
                     /locus_tag="ACHOQV_005064"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFP9633284.1"
                     /translation="MSMNNTVSSEQKTLDIICLGRVAVDLYGQQIGSRLEDMTTFAKY
                     LGGSSGNVAYGTAIQGLRSAMLARVGDEHMGRFIREELNRVGVNTQCLLTDPERLTAL
                     VILGIKDQDTFPLIFYRDNCADMALAPEDIHETFIASSRALAVTGTHLSHINTRAAVL
                     KALTYAQKHGLRRVLDIDYRPVLWGLTSLGDGETRFIASPQVTEQLQQVLHHFDLIVG
                     TEEEFHIAGGSTDTLTALRRVRQLTSAVLVCKCGALGCSVFEGNIADDWSQVQMHSGV
                     RVDILNVLGAGDAFMSGLLRGYLNDEGWEQACRYANACGALVVSRHGCAPAMPTKQEL
                     DDYLSREHAITRPDKDPHLNHLHRTTTRKQHWPELCVFAFDHRNQLVDIARATGASEF
                     TIPELKMLLLEGARQAAHEAGLLHNCGILADTTFGQKALNDVTGQGWWIGRPIEMPGS
                     YPLKLEHGNIGSQLVSWPKEHVVKCLVFYHPLDAESLRLKQEALIDEIYRACCQSGHD
                     LLLEVILPRDAADQSEHYYTQIVERFYLLGIQPDWWKLPPLSPARWQEISQHIERYDP
                     ECRGILILGLDAPESELKSGFAAAADIPWVKGFAVGRTIFGEPSRQWFSGQINDEQLI
                     AAVKQKYRTLIDYWREYRPAL"
BASE COUNT          726 a          683 c          707 g          667 t
ORIGIN      
        1 taaagtatgt tgtagagaaa agtttgtctg aagtggccta ctgaaatgaa ttgcgcagga
       61 aagtgagacg gaagatcggt cagaccgaca ccagcaaaat atgatatatc cgacgaccat
      121 caaaggtcat aaaggcgata aggaataggt gcgcagatat ccgtcatggc tctgcaaaaa
      181 actgtaaaat gaagccgaat gtacgggggc agtctgaacc ccaactgaag catctttttt
      241 gcacaaaatc gggagtccat gtacggatat aaagctgcaa cctaccgtca atgtcagaac
      301 aatggaagct gtgaaaaacg ggatgcgtta atataaactc catgacagta gtggaacggg
      361 ttaatcaagc aggattacca gcaagatgag ggagctgatt cagcacaggc gacctgaccc
      421 tggccacgga taaccgtctg tcgcccgtct gaggtgtcgt tttttcgttc tgccacccct
      481 cggttgagta aaatggaaat taaccgtcaa gctgcctgta gcgtaccgat cagaagattg
      541 ctcagatggt acagctgttg tacgttctgc ggcaacgtga cgagggaact accaatagcc
      601 agacagttca gaaatatcat taatgcagat acgaaacagc actgtcggga tgatgaggaa
      661 agtaggacgg tcatgaaaca tctttgaata cgccccgagc aagaatacag acgcgggacg
      721 taatgcagac ggatactaaa gcgccggacg atattcgcgc cagtagtcga tcaatgtgcg
      781 gtatttttgt ttcactgctg cgatgagctg ttcatcgtta atctggccgc tgaaccactg
      841 ccgtgatggc tcgccaaaga tggtgcgacc tacggcgaag cctttaaccc atggtatatc
      901 cgcagcggcg gcaaagccag atttcagctc gctttccggt gcatccagtc caagtattaa
      961 aataccgcgg cattcgggat cgtagcgctc aatatgctgg ctgatttctt gccaacgagc
     1021 cggacttaac ggtggaagct tccaccagtc aggctggatc cccagcaggt aaaaacgttc
     1081 cacaatctgt gtatagtaat gctcgctttg atctgctgcg tcgcgtggca agatgacctc
     1141 cagcagcaga tcgtgtccag actggcagca ggcccggtat atctcatcaa tgagtgcttc
     1201 ttgcttaagg cggagggatt ctgcatccag tgggtgatag aagacaaggc atttcacgac
     1261 gtgctctttc ggccaactga caagttgtga accgatatta ccgtgctcca gcttcagagg
     1321 atatgacccc ggcatttcga taggacgtcc aatccaccat ccctgaccag tcacgtcgtt
     1381 aagtgctttc tgaccaaaag tagtatccgc caggatgccg cagttatgca gtaatccagc
     1441 ctcgtgcgcc gcctgacgtg cgccttccag tagtagcatc ttcagctcag ggatagtgaa
     1501 ctcgctggca cctgtcgctc tggcgatatc caccagttga ttgcgatggt caaaggcgaa
     1561 tacacacagt tccggccagt gctgttttcg ggttgtcgtt cggtgaagat ggttaaggtg
     1621 agggtctttg tcgggtctgg tgatagcgtg ctcacgggag agatagtcat ccagttcctg
     1681 tttggttggc atcgcggggg cgcagccgtg gcgcgataca actaatgcgc cacatgcgtt
     1741 agcataacga caagcttgct cccaaccttc atcgttcaga tagcctcgaa gcagtcccga
     1801 cataaaggcg tctcctgcac ccagcacatt taggatgtcc acacgtacac cgctgtgcat
     1861 ctgcacctga gaccagtcgt cggcgatgtt tccttcaaat actgaacacc ctaatgcgcc
     1921 gcatttgcat accaataccg cagaggttag ctgacgcaca cggcgcaggg cggtcaacgt
     1981 atcagtactg ccgccggcga tatgaaattc ctcttcggtg ccaactatca gatcaaaatg
     2041 gtgcaatacc tgttgaagct gttcagtcac ctgtggtgaa gcgatgaaac gtgtttcacc
     2101 atcccccagc gatgtcaacc cccatagtac cggacgataa tcaatatcca gcactctacg
     2161 cagaccgtgt ttttgagcat aggtcagcgc tttaagcact gcggcgcggg tattgatgtg
     2221 agaaagatga gtgccggtga cggccagggc acgtgaagag gcaatgaatg tttcgtggat
     2281 atcctcaggc gccagcgcca tatccgcaca gttatctcga taaaagatca gcgggaaggt
     2341 gtcctgatct ttaataccaa ggatcaccag ggctgtcaga cgttcaggat cagtcaacag
     2401 gcattgtgta ttgacgccaa cacggtttaa ttcttcgcgg atgaaacgtc ccatatgttc
     2461 atcgcccaca cgtgccagca tggcggaacg tagtccctga attgctgtgc cgtaagcgac
     2521 gttgcctgat gagcctccta aatattttgc aaaggttgtc atgtcttcca gacgtgaccc
     2581 gatttgttga ccatataaat caacggcgac acgccccaga cagataatgt ccagcgtttt
     2641 ttgctcactg ctgactgtgt tgttcataga gatatcctcc tacagaagac cgtgtgattt
     2701 ttgtttacac taaggttata taatttcgat ttcaacaaaa aggaaaaaat aattccatta
     2761 ttttttgacg ataatcatgc ttt
//
LOCUS       DAZKGF010000328         2753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZKGF010000328 DAZKGF010000000
VERSION     DAZKGF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.329"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            69..1280
                     /gene="bluF"
                     /locus_tag="ACHOQV_005065"
     CDS             69..1280
                     /gene="bluF"
                     /locus_tag="ACHOQV_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFP9633285.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELMCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALDLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVTTPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
     gene            1495..2226
                     /gene="bluR"
                     /locus_tag="ACHOQV_005066"
     CDS             1495..2226
                     /gene="bluR"
                     /locus_tag="ACHOQV_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFP9633286.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2447..>2753
                     /locus_tag="ACHOQV_005067"
     CDS             2447..>2753
                     /locus_tag="ACHOQV_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFP9633287.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRVAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTKKNPAKIKEAALLHISGKSTFREYCSTLADAGVFR
                     "
BASE COUNT          791 a          593 c          607 g          762 t
ORIGIN      
        1 aagaaagaca ccggaagata tgtctgttac cgtcttactc tcgcctctcc cattaccctg
       61 gacagttaat gcttaccacc cttatttatc gtagccatat acgtgacgac gaacctgtca
      121 aaaaaatcga agaaatggtt tcgatagcaa atcgcaggaa catgcagtct gacgtaacag
      181 ggatcttact gtttaatggt tctcattttt tccagcttct ggaaggtccg gaagaacagg
      241 ttaaaatgat atatcgggct atatgccagg atccacggca ctataatatt gttgagctga
      301 tgtgcgatta cgcgcctgct cgccgttttg gcaaagcggg aatggaatta tttgatttgc
      361 gcctgcacga gcgagatgac gttttacagg ccgtattcga caaaggcaca tcaaaatttc
      421 agctaactta tgatgacaga gcgctacaat tttttcgtac ttttgtcctt gcaaccgaac
      481 aagcaaccta tttcgagatc cctgccgaag actcctggct ttttatcgct gacggatctg
      541 ataaagaact tgattcctgt accctttcac caaatataaa cgaccacttt gcctttcatc
      601 ctattgtcga tcccttatcg cggcggataa tcgcttttga agccattgtg caaaaaaatg
      661 aagacagccc gtcagctata gcggttgggc agcgtaaaga aggggaaatc tatacagcgg
      721 atctcaaaag taaggcgctt gcattcgcga tggcacacgc acttgacctc ggtgataaaa
      781 tgatttcaat caatctatta cctatgaccc tggttaacga acctgacgca gtctcttttt
      841 tacttgatga aataaaggcc aatgctctgg tgcctgaaca aatcatcgtt gaatttactg
      901 aaagtgaagt catatctcgg tttgatgagt ttgccgaagc ggttaaatcg ctaaaggctg
      961 ctggtatcag tgtagcaatt gatcattttg gcgcaggttt tgccggtttg ttactcctgt
     1021 cacgcttcca gcctgacaga attaaaatca gtcaggaatt gattaccaat gtccataaaa
     1081 gtgggccacg gcaggcaatt attcaggcga tcataaaatg ctgtacatca cttgaaattc
     1141 aagtctctgc tatgggtgtg acaacaccag aagagtggat gtggcttgaa tctgcaggaa
     1201 ttgagatgtt tcagggagat ctgtttgcga aagctaaatt gaatggtatc ccttcagttg
     1261 cgtggccaga gaaaaaataa ttttcagcac attatttcac atgatttcac atgatttccg
     1321 taaatcatcg tgaaaatgaa tacgttatgt ttaaatttaa tccacattaa aaataatgtt
     1381 tcattcaatt tagctgaaat cttatttata ctttgtacaa ttttagcgta tattgtacaa
     1441 agtattattg ggtcgtgtac aggcgacgga gatttgtgac cgcaaggagg aattgtggct
     1501 tattacagca ttggtgatgt tgctgaacgt tgcgggatta atcctgtcac tctccgggcc
     1561 tggcaacgcc gctacggttt gttaaaacca cagcgcagtg aaggcggaca ccgactcttt
     1621 gatgaagaag acatacaacg catcgaagag atcaagcgtt ggataagtaa tggcgttcct
     1681 gtaggcaaag ttaaagcatt actggaaacc accagccagg atacggaaga tgactggagt
     1741 cgcctgcaag aagagatgat gtcaattctt cgtatggcta atcctgccaa actacgcgcg
     1801 agaattattt cactgggtcg agagtaccca gtagatcaat tgattaatca tgtttacctt
     1861 cctgttcgcc agcgtctcgt gcttgatcac aacacctccc gcattatgag cagtatgttt
     1921 gacggcgcat taattgaata cgcagcagcc tcacttttcg aaatgcgccg taagcccggt
     1981 aaagaagcca ttctgatggc gtggaatgtt gaagagagag cacgattgtg gctggaagca
     2041 tggcgtttat cattgtcagg atggcacatt tctgttcttg ctgatcccat tgaatcgccg
     2101 cgcccggaac tgttcccgac gcaaacattg attgtctgga caggcatggc accaacgaga
     2161 aggcaaaacg aacttttaca acactggggt gagcaaggtt ataaagtcat ctttcatgcc
     2221 ccctaatttc acctttatcc cgccccttag acagaatctt ataatctgaa cccacaacag
     2281 cttttgtacc ggagctggtc cgttgcaaaa tgctcaccgg gagaattcag gtaatccccc
     2341 tttcacactt tgtacatatc acagccaaag gacagaccag attgaagtgg taaaaccgat
     2401 cgtgcacact acgcagtctc acaactaaca agaggttatt atcctgatgg atcaggtcgt
     2461 tgtttttcaa aagatgtttg agcaagtgag aaaagagcaa aacttctcct ggttttattc
     2521 agaattaaaa catcaccgcg ttgcacatta catttattat ctggctacgg ataacatcag
     2581 aattattact cacgatgaca cggttttgtt attaagagga accaggaacc tgttaaaagt
     2641 tagtacgaaa aagaaccctg ctaaaataaa agaggccgca ttgcttcata tttccggaaa
     2701 atctacattt cgggagtact gttcaacact ggcagacgcg ggcgttttcc ggt
//
LOCUS       DAZKGF010000329         2734 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZKGF010000329 DAZKGF010000000
VERSION     DAZKGF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.330"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1363)
                     /gene="emrY"
                     /locus_tag="ACHOQV_005068"
     CDS             complement(<1..1363)
                     /gene="emrY"
                     /locus_tag="ACHOQV_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFP9633288.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIM"
     gene            complement(1363..2526)
                     /gene="emrK"
                     /locus_tag="ACHOQV_005069"
     CDS             complement(1363..2526)
                     /gene="emrK"
                     /locus_tag="ACHOQV_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFP9633289.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTTPIEKEISNIISHNGQL"
BASE COUNT          831 a          588 c          544 g          771 t
ORIGIN      
        1 ccataatttg tgacgatgaa ttaaatacgg ggttaaattg atcgatggtt gctgtcaact
       61 gactatggtg taacgattcg cgtcgtcccc acagcgtcat tgtcaacgac gtaccaactg
      121 atcctgacaa ggtacgaaaa aaattactca tactcgaggc attggcaaat ttattatctg
      181 gcaaccctga aaacgaaatc gttgttaagg gtaaaaagaa acaggcaacg gcgaatccct
      241 ggaaaaactg tggcaaaatg atgcctgtaa aatcaatcgt tggcataaat gtcacagaac
      301 gccagtaata gcaaaccgca tacatcaaaa aactaaatgt caccaacaac cgcatatcaa
      361 ttttgttgcc ataacgtcct atcaaaggtg atattaatag cggcatgatg ccgatgggcg
      421 cataggcaag tccggcccat atcgcattat accccatcgt ttcctggagt aactgcggca
      481 taaggactat cgccccagag taaaataaat acgcgcatgt gatactcaca ataccaatgg
      541 tgaaattacg ggacttaaac aaactgagat caagaatcgg attctctgag gtcgactccc
      601 aaatgactaa agaaatcaga gaaataactg acactactgt tagtattatt attgtactcg
      661 agttgaacca atccagatcg cgccctttat caagcattat ctgcaagcca ccaacaccga
      721 gcactaacag ggtcagtcct ggtagattca ttttgaccgg tgaagtctca gtttctcttc
      781 ctttaagtaa ggttaagcat aatgtcagaa cgataatccc catagggaca ttgattaaaa
      841 atatccaacc ccagctaaag ttatcgcaaa tatagccgcc caatatcggc ccacatatcg
      901 gagcgataat tacggtcatt gaccataatg ccaaagcaaa tgttcttttt tctggcggat
      961 aattccttaa taataaactc tgtgacagtg gaattaacgg ccccgccatt aagccctgaa
     1021 cgactctaaa aaatatcagc acatcaagat tggtcgatag gctacacatt aatgaagata
     1081 gtgaaaaaaa ggttactgaa agtaaaaata atcttaattc gcctattctt tgtgccagcc
     1141 tgccggtaac agggatcgca atggcatttg ctacgccaaa cgaagtgata acccaggtgc
     1201 cttcgtctgt tgatgctccc agaaagccag atattgtcgg tattgcgacg ttagaaatag
     1261 tggaatccaa catttgcata aacgtcgcta aggacaatgc aatagtaacg caccataacg
     1321 tcccaccggt taatggtgcc ggagttgatt tagtgattgc cattaaagtt gtccattatg
     1381 cgaaataatg ttgctaattt ctttttctat cggagtagta tcgataacta aagccttact
     1441 ggtataagca ggcatggagg tcacggttga agccagctca ggcatctcag caatgtcttc
     1501 gtccttcgta tcaatagttg cagtcatcga taaaccaata cgtaagggat gttccatgag
     1561 ttcttttgga tcaagagaaa cttcaaccgg tacacgctga acgattttga tccagttccc
     1621 tgtcgcattt tgtgcaggta ataaggagaa cgcattgccg gttcccatat tgattcctgt
     1681 cacccgacca tgaaacacaa cattttcacc ataaagatcg ctgataatat tgaccgattg
     1741 accaatccgt acatccgtga gttgtgtttc tttaaagttg gcattaaccc acatttgacg
     1801 tgccggtacg acagccatta acgattgtcc gggactcact gtttcgccga cctgaacact
     1861 tcgctgggca atatagccgg taacaggact cttaatatcc gtacgtttaa gcgctaacca
     1921 ggcttctttg gttgcatctg ccgcttcaac gacttgtggc tgacggttta atggtgtgtt
     1981 cattactaaa gctttattcg ctttataagc ctggatagcg gcattcaatg ccgctttgct
     2041 acttattaac gtatctttgg tatgctccag cgtttctttt gaaataaccc cctgcttcgc
     2101 taaaggcaca cgacggttat aatcttctaa agattgttga tactgaatac gtgctgaagc
     2161 gacttcggca ctgtattgtt tatcctgtaa gtacagttta ttcgtttgcc gaacaatatt
     2221 tgccagatta tttttagctt tattgagtgc gatagtggca tcagtcttat ccagtgagac
     2281 taaaatgtca ccctgtcgaa cgtagttcgt atctttatga ttaacgacag tgacgctgcc
     2341 tgaaacttgt gcagaaattg gatctgcatt ccccgtgaca taggcgtcat ctgtactaat
     2401 catgtcttct aattccattg accaataggc ataggcacct gaaaacgcaa taaataaaac
     2461 taccgccaat aaagaaaagt attttcttct gttagaatgt tttttatttg aattaatctg
     2521 ttccactttt atctctcatt tcgcatagat gaaatttatg aattgaatga aaagagtatt
     2581 cattcttcat atcaaacaca gtgcatgtgt tgcacattat cccaggaacg ttggaggcct
     2641 aatttaaatg aggtgcaaaa aaaacaacag aggactaaac cgtggctttt gcaatacaat
     2701 tcttacgcat gtaggattag tgagaagact tata
//
LOCUS       DAZKGF010000330         2725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZKGF010000330 DAZKGF010000000
VERSION     DAZKGF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.331"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2390)
                     /locus_tag="ACHOQV_005070"
     CDS             complement(<1..2390)
                     /locus_tag="ACHOQV_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP9633290.1"
                     /translation="MSIPALNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQ
                     FTSTDKAITPELVLMQDGAFSLTAPPVQGMPVQAPLRTLYGVITGFKHLSSSQDEARY
                     EVRLEPRMALLARSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQV
                     MQYGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGL
                     FDGETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYA
                     DNFLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAV
                     FRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPAFIPRPVMAGTLPARVTSTVK
                     NDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSI
                     AFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVST
                     EYGGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREM
                     APAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAVILLSAPKGV
                     AIASGEHLQLAAAKNVIANAGNNADVGVVKNMFIGVGQALSVFVRKAGIKLFANKGAI
                     SVQAQNDLMELLAKKEIIITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEY
                     NVKAGYYGRKPKAKLTPELMAFPVIEGGDYSRCFKFFDDENIPYRNVKYIANFIDGT"
     gene            complement(2438..>2725)
                     /locus_tag="ACHOQV_005071"
     CDS             complement(2438..>2725)
                     /locus_tag="ACHOQV_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633291.1"
                     /translation="YTFTKSFKGNLIDLVTNSSFFVETIKSAGIKEPVPWDVFPELEP
                     DTFGSLQGELEFWWESIWSPFWHSLSPDERNDFLVKNNATPQWREFIEFHC"
BASE COUNT          544 a          784 c          705 g          692 t
ORIGIN      
        1 gttccatcaa taaaattggc tatatactta acattacgat atgggatatt ttcgtcatca
       61 aaaaatttaa aacagcgaga atagtctcca ccctcaatca ccggaaacgc catcagctcc
      121 ggcgtcagct tcgccttcgg cttacgccca taatatcccg ccttaacgtt atattctccc
      181 ggcgttcccg cttctatccc acaggcatcc agccggatgt agctgccacc gccgttaagg
      241 gtgatctttt tcttcgccgt gattttaatt tcatcttcgg tactggtaat gatgatttcc
      301 ttcttcgcca acagttccat caagtcgttc tgcgcctgca ccgagattgc gcccttattg
      361 gcaaacagct tgatgcccgc tttacgcaca aacacgctca gcgcctgtcc cacgccgata
      421 aacatatttt tcaccacgcc gacatcggcg ttgttgcctg cgttggctat cacattcttt
      481 gccgccgcca gttgcaggtg ctcgccgctg gcgatcgcca cgcctttggg tgcgcttagc
      541 aggatcaccg cctgcgtcag atcttttaca ctctgctgca acagcgctat ctgtgatgac
      601 agatctgccg gatcggcatt cgccgtctgc gcatcagtgg acagcgactt catctgtgac
      661 tgtgcgccat ccagtattgc cattgccgga gccatttccc gcacctgacc ctgagccttg
      721 tcctgcgcat cggcgctgat aaagatgcct ttcttcgccc gcactgcgcc ccacatatcg
      781 gtacgcagtt caaagccttc gccgcgctgc tgtttcccgg catccaccag atggccgagg
      841 tttagctggc tcttgccgcc gtactcggta ctgaccttga tatgctcttt gccgcgctca
      901 tcgtcgaggc ggattttgtt gtttgctggg gtgcgcagca cgttacgctt gtagttctgg
      961 atggtcacat ggtcggtgtg cgccgagtcg tgctttaccc cggcgatata cggtcggtcc
     1021 gggttgcctt cctcaaacgc gatgctgacc tccgttcccg ccagcagcgg cagatgcagg
     1081 ccgtaggtgt ctcccgcata cgggcgcgac tggcgtaccc acaggctctc ataacccggt
     1141 ttccacgtat cgcggtcaaa atcgaggttg acccggtagc gcccgtcttt gtcgatatgg
     1201 gcatagatat cgttcttcac cgtgctggtg acccgcgccg ggagcgttcc ggccatgacc
     1261 gggcggggga taaacgccgg gcggtagccg tagcgttctg agtacgggat agcggtaaac
     1321 gtcagttcat agctgcggtc acgcgccgcg ctggccgtca cgccggtaat cagtacgccc
     1381 ttgcggaaca ccgctggcgc gtcgtcgccc tgaaccctga tttccagccc cggcatcagc
     1441 agtgaactgg tgctctggcc tttgagaatg gcctgttcat tcaggtagcg ttcgtggcgg
     1501 atacgggcat aaaacgcgcc gctttccgct gcctctttat cgcctttttg caggaagttg
     1561 tcggcgtagt gataggcttc gccgtaggtg gtgttatcgc ccccggtggc gtcatgctgc
     1621 tctgtcatca tctccgctgt ggcggtgcgg tagttgtagt cccgcgtggt gacgcttttt
     1681 tccaccacgc tgtacgcggt gttcagcccc catacggctt ccgtttcacc atcaaacagc
     1741 cctgaaggat gacgaagcgg cagcgtcagc ccgcgctcat agccgctctg gtcgtcataa
     1801 aactcgacaa cctcaatttt cagccgggcg tcggtggcga agcggaacca gatgcccact
     1861 tccgacagca ggcgtgaaat aaacgtcagg tcgtcctcgc catactgcat cacctgttcg
     1921 cgggccgggt attcgctttt gaggttaaac acgaagtcct gaccgcgcat ctggtgccgc
     1981 tcgcgcagga ttttttcgac gatttgcggc accgtcagat tctggtagat ggcattctgg
     2041 cggctgcggg ccagcagcgc catacgcggc tccagccgta cttcgtaacg ggcttcgtcc
     2101 tgtgacgatg acagatgttt aaagccggta atgacgccat acagggtgcg caggggggcc
     2161 tgcaccggca ttccctgtac cggcggtgcc gtcaggctga atgcgccgtc ctgcatcagc
     2221 accaactccg gcgtgatggc tttatcggta ctggtaaact gaatgtcgta gcggaacggg
     2281 gtactcagtt gttcgcggcc ttcgaaggtc agcacatcca ccggactttg caggccgcgg
     2341 attttgagtt tgtggtgtga atggtcaaag tttagggcag gaatactcat cattatttcc
     2401 ctgtatagat agaaatattt tcgtcattaa aaataaatca acaatgaaac tcaatgaact
     2461 ctcgccattg tggtgtcgca ttatttttca ctaagaaatc gtttctttca tcaggtgata
     2521 gactgtgcca gaacggagac cagatactct cccaccagaa ctcaagttct ccttgtaagc
     2581 taccgaaagt atcgggttcc aactcaggaa aaacatccca tggtacaggt tcttttattc
     2641 cagcagattt aatagtttca acaaaaaaag aagagttcgt cactaaatca atcaaattcc
     2701 ctttgaatga tttagtgaat gtgta
//
LOCUS       DAZKGF010000331         2711 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZKGF010000331 DAZKGF010000000
VERSION     DAZKGF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.332"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            228..1784
                     /locus_tag="ACHOQV_005072"
     CDS             228..1784
                     /locus_tag="ACHOQV_005072"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFP9633292.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSHMENGDDSICYAKLDDNVITDDIKDDAIRTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLNLGDFNKHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWTGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSARGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTRVQFIDASELFKKEANNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSSYVEAKDTREIINIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCGVQK"
     gene            1877..2711
                     /locus_tag="ACHOQV_005073"
                     /pseudo
     CDS             1877..2711
                     /locus_tag="ACHOQV_005073"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT          797 a          570 c          639 g          705 t
ORIGIN      
        1 caccggaaag gaggcggggg actcacaacg ataatatctg tctgaaatta tttagaatgt
       61 acctggtgca ctgcaaagga aaggcagtat tccgttcctc ctccttgtcg tgaatacccc
      121 aggtatctca caatgtacac cgacttactc cggggctgcc tgaaccacaa gattgctgta
      181 gaattgcccc caatgattca ggtccagacc aggaaaatgg attaaaaatg acaagtattc
      241 aacaacgagc agaactgcac cgccagatat ggcaaattgc caatgatgtc agaggttctg
      301 ttgatggatg ggattttaag caatacgttc tgggcgcact tttctaccgt tttatcagcg
      361 aaaatttttc cagccatatg gaaaatggtg atgacagcat ctgttatgca aaactggatg
      421 acaacgtaat tactgatgac attaaagacg atgccatcag gactaaaggc tacttcatct
      481 accccagtca gcttttctgc aacgtagctg cgaaagcaaa taccaatgac agactgaatg
      541 cagatttaaa cagcatcttc gttgctatcg aaagttctgc ttacggttat ccttcagaag
      601 ctgacatcaa gggtttgttt gctgatttcg ataccaccag taaccgcctg ggtaacaccg
      661 ttaaggataa aaatgcccgc ctggctgctg ttctgaaagg ggttgaaggg ttaaaccttg
      721 gtgacttcaa caaacatcag attgacctgt ttggcgatgc ctatgaattc cttatttcta
      781 actatgccgc aaatgccggt aagtcaggcg gcgagttctt tacaccacag cacgtctcta
      841 agctgattgc acaactggct atgcacgggc agacccacgt taacaaaatc tacgacccgg
      901 cagcgggttc cggttcgctg ttgttgcagg cgaaaaagca gtttgatgac catatcatcg
      961 aagaaggctt ttttggtcag gaaatcaacc atacgaccta taacctggcg cgtatgaaca
     1021 tgtttttgca caacatcaac tacgacaagt ttgatatcaa gctgggcaat acgctgactg
     1081 agccgcactt cagagatgaa aaaccgtttg atgccatcgt ttctaacccg ccgtattcgg
     1141 tgaaatggac tggcagcgat gacccgacac tgattaacga tgagcgtttt gccccggctg
     1201 gcgttctggc ccccaaatcc aaagctgact ttgcgtttgt attacatgcg ctgaactatc
     1261 tttcggccag aggtcgcgct gcgattgtct gcttcccggg tattttttac cgtggcggcg
     1321 cagagcagaa aatccgtcag tatctggtcg acaataacta tgtcgaaacc gtgatttcac
     1381 tggcaccgaa cctgttcttt ggcaccacca ttgccgtaaa catactggtg ctgtctaaac
     1441 ataaaacgga taccagagtt cagtttattg atgccagcga actgttcaaa aaagaggcta
     1501 acaacaacat tctgaccgat gctcatatcg aacagattat gcaggtattt gccagcaagg
     1561 aagatgttgc ccatctggcg aaatcagtcg cgtttgagac cgttgtcgct aatgactata
     1621 acctgtcggt gagcagctat gtggaagcga aagatactcg tgaaattatc aatattgctg
     1681 agctgaatgc tgagctgaaa accacggtca gcaaaatcga ccagttgcgt aaagatattg
     1741 atgcgattgt ggctgaaatt gaaggctgcg gggtgcagaa atgagcgagt tgagttatct
     1801 ggaaaaattg ctggatgggg ttgaggttga gtggttgcca ttgggggaag tctcagcctt
     1861 aagaagaggc agagttatgt caaagggata tttaactgaa aattttggcc catatcctgt
     1921 ctatagctct caaactgcaa ataatgggaa aataggttct atcaatacct tcgattttga
     1981 tggcgaatat ataagctgga caacagatgg cgcaaatgca ggcactgtgt tctatcgaac
     2041 aggtaaattt tcaataacta atgtttgcgg cctaattaca ttaaaaagta aatatccatt
     2101 aatctacaaa tttctgtttt actggctaac aatagaggcc aaaaaacatg tttactcagg
     2161 aatgggaatc ccaagttaat gagtcatcag gttgaaaata tacctgtacc aatcccctgc
     2221 ccggataatc cggaaaaatc ccttgccatc cagtctgaaa tcgtgcgaat tctggacaaa
     2281 ttcagcgccc tgacagcaga gctgacagca gagctgacag cagagctgac agcagagctt
     2341 aacatgcgta aaaaacaata caactactat cgagataagt tgttgaattt caaagatatt
     2401 gcgggggggg gtaattacat ccttccggac gccatggatg gtattcggat tgagtggaag
     2461 actttggggg aagtggctgt tataggaaca ggtaaccatg atacgcaaga tgctatagaa
     2521 catggcaaat atatttttta tgcacgtgga cgtgaaccat taaagttaaa tgtatttgat
     2581 tttgatgaaa cagcaatcat tacggctgga gatggtgccg gggtggggaa ggtatttcat
     2641 tacgctaaag gtaaatatgc tttgcatcag agagcatatc gtattgtccc aaatgcattt
     2701 atgaatccgc g
//
LOCUS       DAZKGF010000332         2707 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZKGF010000332 DAZKGF010000000
VERSION     DAZKGF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.333"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            268..951
                     /locus_tag="ACHOQV_005074"
     CDS             268..951
                     /locus_tag="ACHOQV_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP9633293.1"
                     /translation="MNLKKTLLSVLIIVPLCLLAGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPTDAKAL
                     AKWQQLLFNTTLNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQSA
                     ETRQRKNKK"
     gene            995..>2707
                     /locus_tag="ACHOQV_005075"
     CDS             995..>2707
                     /locus_tag="ACHOQV_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFP9633294.1"
                     /translation="MNKIFKVIWNPATGSYSVASETAKSRGKKSGRSKLLISVLVASG
                     LLSSFGALANNYDGQPTDYGDGSAGDGWVAIGKGAKANTFMNTSGASTALGYDAIAEG
                     EYSSAIGSKTLATGGASMAFGVSARAMGDRSVALGASSVANGDRSMAFGRYAKTNGYT
                     SLAIGDSSLADGEKTIALGNTAKAYEIMSIALGDHANASKEYSMALGASSAASAANAI
                     AVGRNSAAAGVDSLAFGRLSAANAANAIAMGAESKAAENATAVGTNAEANGLNSIALG
                     SGSIADVDNTIALGNQSQAVAAGAIAIGQGNKADGANAIALGNGSITGGVNAIALGQG
                     SYAGLENGTAIGAQASAQGKNSVALGAGSVATDADTVSVGNTTAQRQIVNMAAGDIST
                     TSTDAINGSQLYAISKSVADNLGGGATVNAQGVVTSPNYRLKSGIFGTVGDALTGLDN
                     NTLQWDSLKKAYSAAHGTDTTSTITNVKDGAISDTSKDAVNGSQLKTTNDNVATNTAN
                     ITTNTNSINTLTDSVGDLKDDALLWNGTAFSAAHGTEATSKITNVKDGDLTAGSTDAV
                     NGSQL"
BASE COUNT          791 a          597 c          666 g          653 t
ORIGIN      
        1 ctgaatattt acctttccct gccctgggcc tttatccggt gaaacatttg acattgattg
       61 acaccgatca ttaaaatccg cgcgattaat attattggct acaaaccgaa attgtttgac
      121 acttaataac atattcgcac aaccgattaa tacaaaaatt ctctaacaaa tttatttgtc
      181 ggatatgctc cactctaatc attcataaaa aaaatgattg tgccgtaatt atcaactgta
      241 tcgggattat gaaaaggaat tcatcctatg aacctaaaga aaacactgtt aagcgtgtta
      301 ataatagtgc ctctctgctt attggcaggg tgtgactata ttgaaaaagc gagtaaggtc
      361 gacgacctcg ttacacagca agagttgcaa aaaagcaaaa ttgaggcgct tgagaaacaa
      421 caggaactcg ataagcgaaa gatagaacat tttgaaaaac aacaaactac tatcataaat
      481 agtaccaaga cgctcgctgg cgtggtgaag gcggttaaag acaaacagga cgaatttgtc
      541 tttacagaat ttaacccggc acaaacccaa tacttcattt taaataacgg ctctgttggt
      601 ttggcaggcc gggtgctggc tattgatgca gttgaaaatg gcagcgttat tcgtatttcg
      661 ctggttaatt tattaagcgt tcccgtatca aatattggct ttcatgcgac atggggcaat
      721 gaaaggccca ccgatgccaa agctctggca aagtggcaac aattactttt caacaccaca
      781 ctgaattcca ctctgcaatt aatgcctggt caatggcaag acattaatct gacattaaaa
      841 ggcgtttcgc ctaataacct gaagtatctg aagttgtcca tcaatatggc caatctccag
      901 ttcgacactg ttcaatctgc tgaaactcgg cagcgaaaaa acaaaaaata aatgcagctt
      961 ctgcgcttat atcaaggaat agagagcatc aataatgaac aaaattttta aagttatctg
     1021 gaatccagcg acgggcagtt acagcgttgc cagcgaaacg gcgaagagcc gtggtaaaaa
     1081 aagcgggcgc agtaagctgt taatttctgt actagttgcg agtgggttgt tgtcgtcgtt
     1141 tggagcgtta gctaataact acgacgggca gccaactgat tatggcgatg gctcagcagg
     1201 tgacggctgg gttgctatcg gtaaaggggc aaaagcaaat acctttatga acactagtgg
     1261 cgcgagtaca gctttaggat atgacgcgat agccgaaggt gagtacagtt ctgccatcgg
     1321 gtcaaaaacc cttgcaactg gtggagcatc catggcgttc ggggttagtg caagagcaat
     1381 gggtgacaga agtgtcgcgc tgggtgcatc gtcagtagca aatggcgatc gttcgatggc
     1441 ttttggtcgt tacgcaaaga cgaatggtta tacatctctt gctattgggg actcctccct
     1501 tgccgatggt gaaaaaacta ttgcgttagg aaatacggct aaagcttacg aaattatgag
     1561 catcgccctc ggtgatcatg ccaatgcgtc aaaagaatat tcaatggcgc tgggtgcaag
     1621 tagtgccgca tctgcggcta acgcgattgc agtaggccgc aacagtgctg cagctggcgt
     1681 agacagcctc gcattcggta gactatccgc cgccaacgcc gcaaacgcga ttgctatggg
     1741 tgcagagagc aaagctgctg aaaatgcgac ggcagttggt actaacgcgg aggcaaatgg
     1801 acttaatagt atcgcgttgg gctccggcag tattgcagat gtcgacaaca ctattgctct
     1861 gggcaatcaa agtcaggcag tagcggctgg cgcaattgcc atcggtcaag ggaataaagc
     1921 tgacggcgca aatgctatcg cgctgggtaa tggtagtatt acaggtggtg taaatgctat
     1981 tgctcttgga caaggcagtt atgccggttt agaaaatggc actgcaattg gtgctcaagc
     2041 cagtgctcag gggaaaaatt cagttgctct gggtgctggt tctgtagcga ctgacgcgga
     2101 tactgtttct gtgggtaaca caacagctca gcgacaaatt gtcaatatgg cagcaggtga
     2161 tatcagcact accagtactg atgccatcaa tggatcacag ctttatgcta tcagtaagtc
     2221 agtagcggac aatcttggtg ggggggctac cgtcaatgcg caaggcgtcg ttacttcccc
     2281 aaattacagg ctgaaaagtg gtattttcgg cactgttggc gacgccttaa cgggcctgga
     2341 caataatacg ttacaatggg actcccttaa aaaggcatat agtgcggcac atggtacaga
     2401 tactaccagt accatcacca acgttaaaga cggcgctatt tctgatacca gtaaggatgc
     2461 ggttaacggt tctcagctga aaaccaccaa cgataacgta gcgaccaata ctgccaatat
     2521 caccaccaac actaacagta tcaataccct gacggattcc gttggcgacc ttaaagacga
     2581 tgccctgctg tggaatggca ccgcgttcag cgccgcgcac ggcaccgaag ccaccagcaa
     2641 aatcaccaac gtcaaagacg gtgacctgac ggctggcagc accgacgcgg ttaacggctc
     2701 tcagctc
//
LOCUS       DAZKGF010000333         2696 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZKGF010000333 DAZKGF010000000
VERSION     DAZKGF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.334"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            98..307
                     /locus_tag="ACHOQV_005076"
     CDS             98..307
                     /locus_tag="ACHOQV_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9633295.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPRKEPPAGNHTA"
     gene            complement(327..560)
                     /locus_tag="ACHOQV_005077"
     CDS             complement(327..560)
                     /locus_tag="ACHOQV_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633296.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(649..1272)
                     /locus_tag="ACHOQV_005078"
     CDS             complement(649..1272)
                     /locus_tag="ACHOQV_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9633297.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYHL"
     gene            complement(1361..1870)
                     /locus_tag="ACHOQV_005079"
     CDS             complement(1361..1870)
                     /locus_tag="ACHOQV_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633298.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGINGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWPVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSLM
                     PRGIATIIR"
     gene            2328..>2696
                     /locus_tag="ACHOQV_005080"
     CDS             2328..>2696
                     /locus_tag="ACHOQV_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633299.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPTQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILLR
                     GKETETISERLIREADTREYE"
BASE COUNT          768 a          677 c          561 g          690 t
ORIGIN      
        1 taaaaacaga aaattaacaa cgcacatcag gaaacgtcgt ctcctgattc agtgatacca
       61 catgaaaatt tagtgaacgg ttaccgttct gaagtctatg tcagaagata ttccattacc
      121 aaaaatcaat cagcgataca gagacgatca tggcgcgctt gtcaccgtga ccagtgttga
      181 ggaaacccgg gtggtgttta tgcgggacgg ctatccgcat ccctgcatgc ggccgatgta
      241 caacttcctg ggaaaattca aacccgaacc ccggaaagaa ccccctgccg gcaatcacac
      301 tgcctgatgg cagcataatc cacttattaa ttctcatccc cggagacaag cccagaaaac
      361 cactccgggt gttcactgta tgccgtgttc agcgcatccc aagaaacgtt gatataaaag
      421 ctgtcctgca atttgcgggt aacaatttct gccacccggt tacggttatc cgcctgtccg
      481 gcataacctc tctctgccag ccactcattc agttcatgtt ccggagacag attgataata
      541 tcgtaatcgg accttgccat tcccgtttcc ttagcacatg ttttcaggct cggggataaa
      601 gaatgtcgta ctgctgctgc cagagcaata ttccccctga tggtcgaatc agagatggta
      661 ggccggtact ttgcgggttg ttcccgtcag attatcatgc acctccgcgg tcgttgtcac
      721 caccggcatt ccgctctttc tcagcctcag gacatcagcc gcaatacgct gactaccgaa
      781 ccagaacagg ccgtccagtg cagtcaccag caaccccgcc tccagcgcat gcttcagccg
      841 ttcacggggc gctttcactt cccgggcaat ctgctggtat ggcgatgatg tgttttcatt
      901 cccaatcacc cggcgaatac gatgagacag atgataccgg tatgtatccg gtacaccgga
      961 aaggctggcc ttcaggctgt acacgcagcc aaatcgttta tcattgaaca ccacattttt
     1021 ctggctgatg ccccattctt cacgcagcgc ggcaatcagt tgtggtgtcc gggtaagcaa
     1081 caagcgaaaa ggcagttcaa aactggtgac gtaatccaca ttcagcagtg caatacgcag
     1141 ccgttcttcc ggcccggcct ctgtctgccg gcactcctcc agaacatcct gccactgctg
     1201 gtgaaggcgt ggaagttcat tcagttcagt aaaactgtat ttatccgcaa gatagtcaat
     1261 tcgtgtacgc atactgaaga gtattccgta taaagattca gctggcaaaa ctttatcagt
     1321 ctgtaaaaac taacggaaga gtcgatattt ctcccgtcca tcaccggatg attgttgcaa
     1381 taccccgtgg catcagagac tgaacagccg tttttaacgc cacgtattgc cctgatgaac
     1441 caggccgggc aacccgaaaa catctttcca cccggcattg tccgccagca cttatcaccg
     1501 gccaggtctg ttgcagtaaa tccaccagtt gcgcacattc ttcatcagcc atgacgccga
     1561 ctcgcggatg gcaaatactc gtctggctga gcagcaacag acatcgtatc tgtgctcctt
     1621 catgttgttc ccgcaaccag cgtaatacca ccggcgctga tgggctgacc gtgtgattat
     1681 gctccgtaat acgttcgtgt acccgtcggt gaaaggaact acaaaatgtc tgaatctgtt
     1741 gaccattgat tccttctgcc gaatagagcg tgaagtgaaa tgccaacaga tttggccagt
     1801 ggtccaccgc ctgctgaaca aaacgccgga tttcccccgg ctctgaaatt aaggcttcgg
     1861 ttatttgcac gaaatgttcc ttttcaaccc ctgttgaatt ttgtcaagcc ggtgcaggca
     1921 cagtaaagcg cgcatcaaca cctgaaaggt acgatacaaa cacaagttac aaacaataag
     1981 aacggaagga caattaatca gtgaggaata ataactaaat taaccaaaag aatgttactg
     2041 acaatactac caatacttaa atagcaatat attaattaat gtaagaacag atgcccggta
     2101 agacatatac acaatacatc cacaaacaca ctcttttcat taaatcaatc acctgatcaa
     2161 accatgaaag tgtgatgtaa gtcaaaatta ctgacactat caattgataa tgattatctc
     2221 tatcattatc gtgaaattcg agaatgaaag gagaagtagt cattataagc cttttataaa
     2281 tcgtcatcat catagtaaag tagaacactt tttatatgag ttactatatg aaaatatact
     2341 ggacaagaaa acgcattcct gaactttcag cacttccacc ttcattaaga aagaaaaatt
     2401 ttactgacgc atataatgca gcatcttctc atatcgaata ctggataggt gctggcgtct
     2461 catttatctc gatgatgatt ttatccagag tatatgactt tcttttaccc acccaagata
     2521 ctttcccggg tgatatcatc agaagtttat gtgttgtgtg tccatccata ttaatctggt
     2581 ttcagttttc ggtatatgtc atgaggaaat attacagaca tattctgctg cgaggaaaag
     2641 aaaccgaaac catttcagaa aggttgatac gtgaggctga tacacgagaa tatgaa
//
LOCUS       DAZKGF010000334         2643 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZKGF010000334 DAZKGF010000000
VERSION     DAZKGF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.335"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1558
                     /locus_tag="ACHOQV_005081"
     CDS             <1..1558
                     /locus_tag="ACHOQV_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9633300.1"
                     /translation="FFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAKLIPFLAIP
                     IIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGFMAMHLGTN
                     FYLKQHDLLVVADQMSTHGQNIILGIQSYNTSVLGGIVAGLLVASMYKKIVNLRIPES
                     LGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVGYFFYAVAE
                     RVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIPLDLAGKME
                     QGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIEFLFLFVSP
                     LLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAVCVEVAYFF
                     IYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADTPTLDKSELQAAEQMVKALG
                     GKENIKSLDNCVTRLRLTIADMGLIDEVAIKRAGGIAVVKLDQNTLQVIIGTKVIALR
                     RDMDNYMGMR"
     gene            1558..>2643
                     /locus_tag="ACHOQV_005082"
     CDS             1558..>2643
                     /locus_tag="ACHOQV_005082"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP9633301.1"
                     /translation="MDIFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYHQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNILVFTPVYNAFFDVIKHSERNILTASLIIKNEGGYAINWQDFDLKIK
                     SAKMVLLCNPHNPTGTVWSEEELLKIATSCTRHNVWLCSDEIHSDFVFNRSFTSALKI
                     KKEKVVVFNSISKTFNVPALTGSYLISTDEDYNHKFRTISRYRDFVNSPSVLNIIATI
                     VAYNECEGWLKSLKAHIASNIQFTQQYLNENTPELIVRPADGCYFSWIDCSAIGYSFD
                     EFYSRLIHEGKVGIMAGNVYGTEGEGYL"
BASE COUNT          718 a          493 c          609 g          823 t
ORIGIN      
        1 tttttttcag agcctgggga aggcatttat gtatcccatt gctctgctaa gtgtatgtgg
       61 catgatgcta gggctgggaa gtggtttagc cagtgatgat atggcaaagt taattccatt
      121 tctggctatt ccaataatta aaaccatact tgatttcatt gttagtcttg gtttgtttgc
      181 ctttgttaat ttacctgtat tgtttgcgat agcgattccc ttaggattat taaaagataa
      241 agaggataaa gcctatggtg ctttttctgg cttaattggt tttatggcga tgcatctggg
      301 aacgaacttt tatcttaaac agcacgactt attggtcgtt gctgaccaaa tgtcgacaca
      361 tgggcaaaac atcattctgg ggatccagtc ctacaatacc agcgtgttag ggggaattgt
      421 tgctgggtta ttagtcgcca gcatgtataa aaagatcgtt aatttacgca ttccagaatc
      481 attaggtttt tatagcggcc cacgtctggt gcctatcatt acactgattg tgatgagtgg
      541 atttggtctg atcattcctt ttatctggcc gccatttttc aatctcttca tgctcattgg
      601 ccactggata tcaacttccg gtcctgttgg ttatttcttc tatgcagttg ccgaacgtgt
      661 gacgattcct tttggcttaa accatctggt gacgtcagtg ttccgcttta cgccaatcgg
      721 cggttcggct gtgattggcg gcgaagagta ttacggcacc ctgaacatgt ttatggcgta
      781 cgtcaaagag aatgcggtca ttccgctgga tttggcgggg aaaatggagc agggcaaact
      841 gatgattcag tacggtctgg ctggtgctgc actggccatg tatcgcactg ctcatgctca
      901 aaacagaaag gctatcaaag cattgcttat ttccggtgtg cttacggtta ttattggtgg
      961 cgtcagcgaa ccgattgaat ttctgttctt atttgtcagt ccgctgctgt ttgtcttcca
     1021 tgcctttatg aatggattcg ccaacatggt cttgccatat atgggcgtga agatgggatt
     1081 tactggcgat ctgattcaat ttattagctt tggcgtattg cgtggcacaa gaacaggttg
     1141 gccgatcgcg gtgtgtgttg aagtggccta tttcttcatt tattactttg tgttccgttg
     1201 gaccattctc aaatttaacc tgatgaccgt aggacgtgaa gagtccagtc ctgtcacgct
     1261 gaacgctcac gaagatacgg ctatagcgga taccccaact cttgataaat cagagttgca
     1321 agcagcggag cagatggtta aggcacttgg tggtaaagag aatattaagt cactggataa
     1381 ttgcgtaact cgtttacgtt taacaatcgc agatatggga ttgattgatg aagttgcaat
     1441 aaaaagagct ggcgggattg cggttgttaa acttgatcaa aataccctac aagtcattat
     1501 cggcactaaa gtcatcgccc tgcgtaggga tatggataac tacatgggga tgcgctgatg
     1561 gatattttta atactcctat ttcccgtaaa ggaacatatt gcacccaatg ggacttttgc
     1621 gaggacagat ttggtgtaaa agatgtatta cccttctcta tttcagatat ggatcttccg
     1681 ataccagacg ctatcaccag agcattaaaa aaacgcctgg aacacccgat tttagggtac
     1741 agtcgttggc aacatggtga atatctgaat gcgatagtaa actggtatca tcaacaatat
     1801 caaacagaca ttaaacctga atggataacc tatagcccca gtgtgatgta ctctattgca
     1861 aaggcaatag agttattgac atcgcatggt gataatattc tcgtttttac acctgtctat
     1921 aatgcatttt ttgatgtaat taaacatagc gagcgaaata ttctgacagc ctctttgatt
     1981 ataaaaaatg aggggggcta cgctatcaac tggcaagatt ttgatcttaa gattaaaagc
     2041 gcaaagatgg tgttattatg taatcctcat aatccgacag gaacagtctg gtcggaagaa
     2101 gaactgctta aaatcgctac aagctgtaca aggcataatg tctggttatg ttcagatgaa
     2161 attcatagtg attttgtttt taatcgtagc tttacatccg cgcttaaaat aaaaaaagaa
     2221 aaagtagtgg tatttaactc gatatcgaaa acatttaatg ttcctgcgct aacaggatcg
     2281 tatctgattt caactgacga ggattataat cataagttca gaacgatatc gaggtatcgc
     2341 gatttcgtaa actccccatc agtactgaat ataattgcaa ccattgttgc ctataatgaa
     2401 tgtgagggat ggttaaagtc attaaaggca catatcgcct ctaacattca attcacccag
     2461 cagtatctga acgaaaatac tccagaattg atcgtaaggc cagctgatgg ttgctatttt
     2521 tcgtggatag attgctctgc tattggttat tcctttgatg aattctacag tcgattaatt
     2581 catgaaggca aagtgggcat tatggcgggg aatgtctatg gaacggaggg ggagggctac
     2641 ctt
//
LOCUS       DAZKGF010000335         2615 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZKGF010000335 DAZKGF010000000
VERSION     DAZKGF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.336"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            157..2436
                     /gene="ydeP"
                     /locus_tag="ACHOQV_005083"
     CDS             157..2436
                     /gene="ydeP"
                     /locus_tag="ACHOQV_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFP9633302.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGERLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTIGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLTVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT          694 a          620 c          642 g          659 t
ORIGIN      
        1 atcgtattta atcttttgtt taacgataat agattaaaat agaaagagag tagtccccta
       61 ctcttatata tccatgttga cgataatccc tttgtatata ctgtgcatca tcgctattac
      121 aaatcctaat aattcatttc cacacaggat aagcagatga agaaaaaaat tgaatcctac
      181 cagggagctg ccggtggttg gggtgctgtt aaatccgtag caaatgcagt acgtaagcag
      241 atggatatac gccaggatgt tattgccatg tttgatatga ataagccaga gggctttgac
      301 tgtccgggat gtgcatggcc agatcctaag cacagtgcgt cattcgacat ttgtgaaaac
      361 ggcgcaaaag caatcgcctg ggaagtcacg gataagcagg taaatgcctc tttctttgct
      421 gagaatacgg ttcaatcatt acttacctgg ggagatcacg agcttgaggc tgcgggacgt
      481 ctcactcagc ctttgaaata tgatgacgtc agcgactgtt acaagccatt aagctggcaa
      541 caagctttcg acgaaattgg cgaacgcctt caaagctata gtgatcccaa tcaggttgaa
      601 ttctatactt cgggccgcac ttccaatgaa gctgcctttc tttatcagct ttttgcccgt
      661 gaatacggga gcaataactt ccccgactgc tccaacatgt gccatgaacc gaccagtgtg
      721 ggtttggcag cgagtatcgg tgtaggtaaa gggaccgtgt tgctggaaga ctttgagaaa
      781 tgcgatttag tcatttgcat tgggcataac cctggtacaa accaccctcg catgttgact
      841 tcgttgcgcg ctttagtgaa acggggagcg aaaatgatcg ccatcaatcc tctacaggaa
      901 cgtggcctgg agcgatttac cgcaccgcaa aacccgtttg aaatgctgac gaactctgag
      961 actcagttgg ccagtgccta ctacaacgtg cgcattggtg gtgatatggc gttgctcaag
     1021 gggatgatgc gcctgttaat tgagcgcgat gatgctgcaa gcgccgcagg tcggccctca
     1081 ttgctggatg acgaatttat tcaaacgcat accatcggct ttgacgagct acgccgtgac
     1141 gtgctcaatt ccgagtggaa agatatcgaa cgtatttctg gactaagtca gacacaaatc
     1201 gccgaacttg ctgatgcata tgccgctgcc gaacgaacca ttatctgtta cggaatgggg
     1261 atcactcagc acgaacatgg tacccagaac gtacagcaac tggtcaatct gctgttgatg
     1321 aaaggtaaca ttggtaagcc tggtgcgggt atctgcccac tacgaggtca ctctaatgta
     1381 cagggcgacc gaaccgtcgg tatcaccgag aaaccgtctg cagagtttct ggctcgcctg
     1441 ggtgagcgct atggtttcac cccacctcat gcacctggac atgctgcaat tgccagcatg
     1501 caagcaatat gtacggggca ggctcgagca ttgatctgca tggggggcaa ctttgcactg
     1561 gcaatgccag atcgggaagc aagcgctgta ccgttaacac aattagatct gacggtacac
     1621 gtagccacta agcttaaccg ctctcatctg ctgaccgcac ggcatagcta tattctgccg
     1681 gttctgggac gcagcgagat tgacatgcaa aaaagcggtg cgcaggcggt aaccgttgag
     1741 gattcaatgt cgatgattca tgcctcgcgt ggcgtgttaa aacccgccgg tgtaatgctg
     1801 aaatcagagt gtgcagtggt cgcgggaatc gcgcaggcag cactacccca gagcgtggta
     1861 gcctgggagt atctggtgga agattatgat cgcattcgca atgacattga agctgtgctg
     1921 ccagagttcg ccgactataa ccagcgcatc cgtcatcccg gtggttttca cctgataaat
     1981 gcagctgctg aaaggcgctg gatgacgccg tcaggtaagg ctaatttcat taccagtaaa
     2041 gggctgttag aagatccctc ttcagcgttt aacagtaaac tggtcatggc gacagtacgc
     2101 agccacgatc agtacaacac gacgatttat ggtatggatg atcgctatcg aggtgtattc
     2161 ggtcaacgag atgtggtctt tatgagtgct aaacaagcta aaatttgccg tgtaaaaaac
     2221 ggcgaaagag ttaatcttat tgcgctcacg ccagacggta aacgtagctc acgccgcatg
     2281 gatagattaa aagtggtcat ttaccctatg gctgaccgct cactggtgac ctattttcca
     2341 gaatcgaatc acatgctaac acttgataac cacgatccat taagtggcat tcctggctat
     2401 aaaagtattc cggttgaatt agaaccatca aattaattcg cctcctcatt tcttctgctg
     2461 tcatccgcac agcagaagaa ttcctcattg accattgtcg cgcaatttac tcacatgtgc
     2521 taaattaaac tgctcgttat aagatatata cctccgccta cagctgtaag aaactccgct
     2581 cagtactgaa gcgccagtcc tatttcccct attct
//
LOCUS       DAZKGF010000336         2606 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZKGF010000336 DAZKGF010000000
VERSION     DAZKGF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.337"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(188..740)
                     /locus_tag="ACHOQV_005084"
                     /pseudo
     CDS             complement(188..740)
                     /locus_tag="ACHOQV_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            857..1762
                     /locus_tag="ACHOQV_005085"
     CDS             857..1762
                     /locus_tag="ACHOQV_005085"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP9633303.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGILNTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKK"
     gene            1807..>2606
                     /locus_tag="ACHOQV_005086"
     CDS             1807..>2606
                     /locus_tag="ACHOQV_005086"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP9633304.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYIQKVSKHLSIYSEICNV"
BASE COUNT          763 a          433 c          639 g          771 t
ORIGIN      
        1 tcaactgcca gttccctgaa tgtttgattt gttcaataaa gcctgtttat caataaattt
       61 tctgacgaca gggaatggta tgtttttatt agtttttatg tgttggacaa caagacagaa
      121 taatgatgtc tgttttatgt atacgatata gccactgatt caacgcatta tcggctatat
      181 tgccagtctt atttagtgcc agattcattg tatcgaaggc cgctgccgtc agtagatggc
      241 tggtcagttc tatcagacag accattttta cctgaggata aagtgtctgg tttccaccat
      301 gtatttggcg agtgaagctt tatcgtgttc ttgcgtgtcc tgtgttcgtc agaacacgcc
      361 atcaatggtc agcagattca gatcgcacca gagcggatat ggcgtggtgt tatgccagag
      421 ctgcgctgtt tgagtgaaca cgcagcgtat ggctccgctt cctggccgct ggcgggcatg
      481 gagcacggcg ctactgcctg gcagtatgat gtccagccgg ttcacaatct gatgaaggtt
      541 ccttaagctc atgggccata ctgataattc accagaccat catttccaga ggaagacgcc
      601 gtttgtgtag cgtgacagtg tcggactctt cagggctgtg ggtaatcagt tccagggcaa
      661 gatagtcgca gagggttacg cagagaatag taactggaca ctggatcgat caaccgatcc
      721 ttaactgagc ggcatgacag ctttctgatg gatttttttg ataatcttca cattgtgctt
      781 cttttctgtt ggataatttt tgcacttggt ggtgtactag gtatcccatt atgcagttaa
      841 gtgaaggtga ttaaaaatgc aatgtgaatt taaaggggtc atatcagcat tacccactcc
      901 ttatgaccaa tcccagcaaa tcgatatgga gagtcttcga aaactgatcc gttttaatat
      961 cgaacaaaat attaagggat tgtatgttgg aggctctact ggagaagcat ttcttcagaa
     1021 tgttgcagag cgagaaaaaa ttcttgaaac agtagccgat gaatcagatg gaagactgac
     1081 acttatagcg catgtgggcg gaatcagtac ggcagaaagc gaggtacttg caaaagctgc
     1141 aaaaaaatac ggatatcatg ctatatctgc tgttactcca ttttattatc ctttttcatt
     1201 tgaagagcat tgtatccatt acaggaagat tattgattct gcagatggac ttccgatggt
     1261 agtttataat ataccggctc ttagcggagt gcgtttttct ttagatcaga taaatgagct
     1321 tgtcacaata cccggggttt gtgcattaaa acagacatcc ggtgatttat tccagatgga
     1381 acaaatcaaa aggaatcatc cagagcttgt gttatataat ggttacgatg aaatatttgc
     1441 atcagggtta attgcaggtg cagatggggg aatcggtagt acatataata taatgggatg
     1501 gcgttatctt gaaatatttg aagcagtcaa aaacaatgat gtgattaagg cgaaggaaat
     1561 gcaggtggcc tgtaaccagg ttattgacac tcttattcag agtggtgtcc tggcaggtat
     1621 aaaaactctt ctttactata tggggattct aaatactcct gtatgcagaa gtcctttttc
     1681 tccggtaaag gaaaagaatc tggatgtatt atcaaaactt gcagagcgac tgctcgaaga
     1741 acatgacaga aataaaaaat gaaaataatc tgaataattg caagattgtt atataggtga
     1801 aaatgaatga taacactggc tgttgatata ggaggaacga aaatttcagc ggcgttgata
     1861 tctgatgacg gttcattttt gctgaaaaaa cagatatcaa cacctcatga aagatgtcct
     1921 gacgaaatga caggggcttt acgtctactg gtttctgaaa tgaagggaac agcggagcga
     1981 tttgcagtag catcaacagg gattattaat aatggagtat tgacagctct gaatccagac
     2041 aatcttggag ggctgaaaga gtatcctctg aaaaatataa tggaagatat aactggttta
     2101 aatggaagtg tgataaatga tgcccaggca gccgcatggg cagaatatac agttttgcca
     2161 aaagaaattt gtgatatggt ttttataacc gtttctacgg gggttggagg gggaattgta
     2221 gttaacagga aattattaac aggtgtttct ggattagccg gacatgttgg gcatatattg
     2281 tccggagtaa cggatacaga atgtggatgt ggtcgtcggg gatgcgttga agcggtttca
     2341 tctggtcggg ccatcatggg ggcagcaaaa aatgaacttg caggatatag caccaaatat
     2401 atttttgaat tagcgagaca gggatataag gaagcagaat ttttgacaga gcgttctgcc
     2461 agtactattg ctgaattaat agtaagttta aaattactgc ttgattgtca ggttgttgtt
     2521 gtcggaggaa gtgttggact tgctgatggg tatattcaga aagtgtcgaa acatctttcc
     2581 atatattctg aaatttgtaa tgtaat
//
LOCUS       DAZKGF010000337         2559 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZKGF010000337 DAZKGF010000000
VERSION     DAZKGF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.338"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(234..452)
                     /gene="ymgF"
                     /locus_tag="ACHOQV_005087"
     CDS             complement(234..452)
                     /gene="ymgF"
                     /locus_tag="ACHOQV_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFP9633305.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLINRLRERLVSHRDQH"
     gene            complement(584..2107)
                     /gene="pdeG"
                     /locus_tag="ACHOQV_005088"
     CDS             complement(584..2107)
                     /gene="pdeG"
                     /locus_tag="ACHOQV_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9633306.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIREALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTRSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2443..>2559)
                     /gene="ymgC"
                     /locus_tag="ACHOQV_005089"
     CDS             complement(2443..>2559)
                     /gene="ymgC"
                     /locus_tag="ACHOQV_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFP9633307.1"
                     /translation="DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVIS"
BASE COUNT          773 a          564 c          530 g          692 t
ORIGIN      
        1 caccttattt tcatgacatt gatcactttg gggtgacgat tttgtttaac aaataactct
       61 tcgacatcaa aaccattaat agttgccata gaaattattt caatgatata taaaacaact
      121 gtgataacac cagagttgac aactatttgc gcaataattc ctttcttcaa tgatccaggc
      181 gttcactgcc agcaacccat tcagccgcat actgacagtg agaaactgaa agattagtgc
      241 tgatccctgt ggcttacgag tctttccctt aatcgattaa tcagattgat cgccagccaa
      301 acaagggcat taagcaaatt tatcgtcaca agcacttctc ccagcccggt actgggtttt
      361 gcaataagga tgaccaggag agtgatcagc ataaatgcaa tggaaaagat gatatagaga
      421 gtgaaataat ccagattatt actattgttc atgctactaa tggcaatcct gtcaaaactt
      481 tcaaacaatt atagcaaaca aaataatgaa caatagtaac aagaggcaac gagctaatta
      541 gctgtctata attacaccgc actatggcct tactgatcga ttttcactca accacaacct
      601 tcaccttcgg tttagaaagg ataattttga ccaggtcgat gtatgtaaca ggtttataga
      661 aataataacc ctgctgaaat gtgatattat tttgattcag atagtcaagt tgttctttcg
      721 tttcgacacc ttcagcgacg atactgatcg aaagtttacg cgccagatcc aatacacaat
      781 ccagaattcg ggttgattct gggtctgcgt tgacgcggcc aacgaaactg tgatcaattt
      841 tgatataatc aatatgcagg tcatgcagat aagaaagccc tgagtagcca gtaccgaaat
      901 catccagcgc gatgacaaaa ccattttcat gcagaatgtt caaccgctga accagacttt
      961 catcaacatt taatggctca cgctcggtaa cttccagaac gaggtttaaa tcgcggcggg
     1021 taaaactgtc acggtaattt aagcactcgt cgacaaacgt cggcgaaata atatgcgagg
     1081 cgctaaaatt aattccaata tgaaaacctt ccggcaattt actcgcgata gcgttcatct
     1141 gtctggcaac ctgattcatc aggcttcgcg taagcggaac gattaatccg gatttttcag
     1201 caagtggaat aaatgccgca ggtgatatat atccaccgtg aggttgtttc cagcgggcta
     1261 acacctcaac tccccgcaat gtcccttccc gaccatttac cacaggttgg taaaaaggga
     1321 cgatctcccc attatttata gcccgtcgca ggatctcttc aggcgttgcg cttttattga
     1381 aatacctatc aagcaggaag gcagcggcgc aggcgattaa cagaataaat atcaaaatcc
     1441 caaagccttt atcgataagt ctgtagaagc taaagagtgg cggcgggtta aagataatag
     1501 tgaaatgata ttttgtagag tgaaccttct cggtataggg atagtgtcca ttaagcgtca
     1561 tcacatcacc cgtaggtcca ataatgtcat ctgccacgcg taatacatac cttacccctt
     1621 tcaagggtac attaagcgcc tcgcgaatat gctgatcact tatcgtaacc aaaatgcgcg
     1681 tatctgcaaa ttggctctga tagagcagga taggtaatct attaacggtg tcgattgccg
     1741 gagccagcag taaattactg tccgggaaaa caggaatacg agacagtagg acccgattcc
     1801 ccggtaagga tgtacaccag accgttccct gtttgagaat gatgattgtg cgcaggtgag
     1861 gcttcagagc tgcttcagtg ccaagctgat attgctcctc gaggtcgcat tccttcccgg
     1921 caatgttaac cgccgtttga gtcgcctgtg acgcttcttc aagtatatga ttgatattgt
     1981 tggcggcata tctcgctccc gccaggtact ctgctttcgc ggaataccag agctggatgt
     2041 tcaaaatagc gacaccggtt ataaacaagc atatcgcaac gaggatgggt atgagtgtat
     2101 tgcgcattcg cttgtcccgg acaaagggtt taatacatga aaatgacgtt cattcgctcg
     2161 cgctggcgtc tacagagagc atggtagaga gcatcacaaa tatccacgcc gaagataaaa
     2221 ttttctccaa cttcatctaa cctattaatt attgagcaca atgtaggcag aattaaacaa
     2281 attaggtggg atatcaggcg tcaagaatga aaaacgagcg ctctccattc ctgtttgccc
     2341 cctgatattg cggatatatt aagaaagcat aacgcctgaa atgctcactt tgcaccagca
     2401 tggtgataca gctgatgttt attctataac cttactcaag ttctaagaga tcacggattc
     2461 cctgtcatta ataatgatat ccgttagtgt agtcttatgc atttcaatgt actgcaatat
     2521 ttgcagatag agatcagaaa gaatcacact ggtttcatc
//
LOCUS       DAZKGF010000338         2546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZKGF010000338 DAZKGF010000000
VERSION     DAZKGF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.339"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..575
                     /locus_tag="ACHOQV_005090"
     CDS             <1..575
                     /locus_tag="ACHOQV_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP9633308.1"
                     /translation="EDERIDAINKIKIELHRSSPFYAEPVDCVIWIKNNLIEANDYNP
                     NVMAPSEKRLLTHSLLVDGFTQPVVTLAQKGKYTVIDGFHRQMLGKSTTKLEKKLKGY
                     LPVTCIKHDNNFKAYSIASTIRHNRARGKHQIDALSDIVRDLSRMGWDDLRIGSELGM
                     DTDEVLRLKQISGLTELFEEENFSPAWTIK"
     gene            complement(757..2502)
                     /locus_tag="ACHOQV_005091"
     CDS             complement(757..2502)
                     /locus_tag="ACHOQV_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9633309.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
BASE COUNT          772 a          627 c          517 g          630 t
ORIGIN      
        1 aagaagatga acgcattgat gcaattaata aaataaagat tgagttacat aggagtagtc
       61 ctttttacgc agaacctgtt gattgtgtta tatggataaa aaacaatcta attgaagcaa
      121 acgactataa tcctaacgtc atggctccct ccgaaaaaag gttattaaca cactccctgt
      181 tggttgatgg attcactcaa cctgttgtca ccttagctca aaaggggaaa tacaccgtta
      241 ttgacggatt tcacagacaa atgcttggga aaagcacaac aaaactagag aaaaaactaa
      301 aaggatattt acctgtcaca tgcattaagc atgataataa ctttaaagct tactctatcg
      361 cctcgaccat caggcataac agggccagag gaaagcacca gatagacgcg ctctccgata
      421 tcgtccgaga tctttcccga atgggatggg atgatctccg aataggtagt gaactgggca
      481 tggatacaga tgaggtactg agattaaaac aaataagtgg gttaacagaa ctatttgagg
      541 aggaaaactt tagtccggca tggacaataa agtaatcatt tagggggtga ctttatccag
      601 taattaccga ctgatatatg atcaccccat gtaatggaca gctattgaca gatcaaaaac
      661 atgggggata ttaattcaac tctgaatact cgctaaatac ccatcttaga ttatcaaaaa
      721 acgaatgatt acatatcagc aataataaaa cacaacttat acaaaatcct caagatgacg
      781 actgatactg ttttcacgat aacaggtcac cagtaattcc cgtgtagccc gggtgaacgc
      841 aacataaagc gccggaataa cttcagactc atccgcagca cctgagtgaa cgaaagaagc
      901 attgataacc gcaacatacg gaaactccag tcccttactg ctctggtagg tcagtaaatg
      961 cacaacatcc ttgcgtcgcg aatatttctt cttaccttca ctgtcaaagc aggtggcata
     1021 aggtatcccg cgttgtgtca tgacctcttt cagatgtttc actgaaaact ctgcaggaca
     1081 caaaacagcc atatcactcc agtgaccgca cagtgtatag cgtttctcca gccagcccac
     1141 aacctgcact gcctcatcac tctcagaagc acaacgcaga atttccggct ctgtgccctc
     1201 ttcaccgccc gcctgtggct gaacaaacgg tatttcacgg ttctgatgct tctcaaaata
     1261 ctcacgggag aatgcataag caaagtgcag aatgcgtttc gtattacggt aatttaccgg
     1321 caggacggaa gtacgtccct gcgcctgaat ccccacactg gcaagcgaaa agttcagagc
     1381 ccgttcccgt ctgtaaatgg actgtgcgtc atcgtacatc aggagcagtg aacgggacgc
     1441 gttatcaaac aggcgggcta tcagagccag ccagcgactc tcaaaatcat gcccctcatc
     1501 cacaagtacg gcatcgtatc cagtatcagt aattgttccg ctgttcacgg catcttccag
     1561 cgcactgaag catttctccg ggtaatcctt tcctccagca gtcacctgaa ttccatggcg
     1621 ctttatcatt gaggcgcacc aactatgaaa atggaatacc gtaacccgtg acttcagtcc
     1681 cctggactca atacattccc gcaggtagct ggccagagta atgttgtaac atagcaccag
     1741 aatcttacca ggggtagttt cagccaagta aagacaacgg aacaaaagaa tcattgtttt
     1801 tccagagccg gctaccccat gaataacacg atgcccatca ccaatatttc tggccagaat
     1861 ctcctgctga atatccatta ttttgatttg tgagtgctgt ctgacggtca tttcaggaaa
     1921 cagatgagca cgcagaatat ctctaacctg tggtgtcacc cgggttctga atcccatcgt
     1981 aaacatcccg gcaatttttt ggcggaatat atcaggtaaa acagactgag tcatatcatc
     2041 ctgacagatt gtctgcgctg aagggaaaat cctttccaca gcattttcat cattcccagc
     2101 cagagccttc agttgctggc gggtaatacg ggtaaacaca acaccatacg cccaggccag
     2161 attcagacgc cccttatact gaccatcatt ctgtcgcaaa cgtgagtctg caggtaaagc
     2221 atttacggta tcacaggcat aacgacgaac ctgtagcagc ggatttattt ctgatttaag
     2281 aataccgtta gtttcaagaa tgacctgttc gtgatttacc tgatgcaacg tgtgaacggt
     2341 ccagtccttg acttcaagaa ataccagacc attctccggg tcaataatga caaaatcagg
     2401 atgtcggttt ttccgtccca ccggaatatc gtaccagacc agacactcat tactcataaa
     2461 tgcctcaaga cgggatgcaa cccgcttttc cccggcagtc atccgctcct cggtaacacc
     2521 tgatatattt atcagtttag ccataa
//
LOCUS       DAZKGF010000339         2541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZKGF010000339 DAZKGF010000000
VERSION     DAZKGF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.340"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(87..500)
                     /gene="hns"
                     /locus_tag="ACHOQV_005092"
     CDS             complement(87..500)
                     /gene="hns"
                     /locus_tag="ACHOQV_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFP9633310.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            1104..1721
                     /gene="tdk"
                     /locus_tag="ACHOQV_005093"
     CDS             1104..1721
                     /gene="tdk"
                     /locus_tag="ACHOQV_005093"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9633311.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(2004..>2541)
                     /locus_tag="ACHOQV_005094"
     CDS             complement(2004..>2541)
                     /locus_tag="ACHOQV_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HFP9633312.1"
                     /translation="WPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYS
                     HRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKG
                     YWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRESAFPRVV
                     KERPWKYPTAPKKSQSVA"
BASE COUNT          648 a          569 c          565 g          759 t
ORIGIN      
        1 gcttattctt attaaattgt cttaaaccgg acaataaaaa atcccgccga tggcgggatt
       61 tttaagcaag tgcaatctac aaaagattat tgcttgatca ggaaatcgtc gagggattta
      121 ccttgctcat ccattgcttt tttgattaca gctggagtac ggccctggcc agtccaggtt
      181 ttagtttcgc cgttttcgtc aacgtagcta tattttgccg gacgctgagc acgtttagct
      241 ttggtgccag atttaacggc agcaaggcta ttcagcagtt cgttcgggtc aataccgtca
      301 gcgatcagca tttcgcgata ttgctgcagt ttacgagtgc gctcttcaac ttcagcagca
      361 gccgcgcttt cttcttcgcg acgttcgtta acaacaactt ctaatttttc cagcatttct
      421 tccagcgttt caagtgtaca ttctcttgcc tgcgcacgaa gagtacggat gttgttcaga
      481 attttaagtg cttcgctcat tgtagtaatc tcaaacttat attggggtgg tttgttgagg
      541 taataataga gccttaaatt cagttgtgca atagccagga atgtaaggaa ttcaaaattg
      601 ttctttattt tgtgccgcca ataaatatct tttcataaaa ttagccagaa aagacgcggc
      661 atatagccct atttgcgccg atgatttcgc agcacgtgag gttaaaactt cctgattcat
      721 gtcacatttt atggggagat tatcgtaggc tgacgacctt tcagtcttct gtattagtcg
      781 tgtttacaag aattccctat taagcgaatg atgaaaagta gaacagtcgc aataagagca
      841 gggacttagt attgcactat ctcctggagg tcaacagagg gctattactt gcgcaacagg
      901 ttaaagattg tgaatagtta ccagcagtca tttacccgct tataacaagc gaggcagttg
      961 taatgatagc tcagaaggat tatgcaaggc ttcgtaaggg agaacgcata taccacttct
     1021 gtgcatactg ttgagctgaa aaactgacga attatgataa actccagcca actttaattc
     1081 atatcattga gggcctgtgg ctgatggcac agctatattt ctactattcc gcaatgaatg
     1141 cgggtaagtc tacagcattg ttgcaatctt catacaatta ccaggaacgc ggcatgcgca
     1201 ctgtcgtata tacggcagaa attgatgatc gctttggtgc cgggaaagtc agctcgcgta
     1261 taggtttgtc atcgcctgca aaattattta accaaaattc atcattattt gatgagattc
     1321 gtgcggaaca tgaacagcag gcaattcatt gcgtactggt tgatgaatgt cagtttttaa
     1381 ccagacaaca agtatatgaa ttatcggagg ttgtcgatca actcgatata cccgtacttt
     1441 gttatggttt acgtaccgat tttcgaggtg aattatttat tggcagccaa tacttactgg
     1501 catggtccga caaactggtt gaattaaaaa ccatctgttt ttgtggccgc aaagcaagca
     1561 tggtgctgcg tcttgatcaa gcaggcagac cttataacga aggtgagcag gtggttattg
     1621 gtggtaatga acgctacgtt tctgtatgcc gtaaacacta taaagaagcg ttagaagtcg
     1681 gctcattaac ggctattcag gaaaggcatc gccacgatta ataagaattt ctttactgac
     1741 agggtgagca gggcgctttt atcctgtcag ttcgttttac tcactttatc cggtgtatat
     1801 acccttctcg acagttcttt aacgccgcta tacccctcac agagcgcttt tacagtgacg
     1861 ttgacttgtg acctgtaaag tacaatatcg ctgtgtgtag gcgttataca tcgccgtaaa
     1921 tatgatgcag gcaaatgctg tcggtttatg ggaaagttgc tttgggtaaa aaaaacggcc
     1981 ccagaaggga taatgccagt cagttaagca actgactggc tctttttcgg ggctgtgggg
     2041 tatttccagg gcctctcctt taccactctc gggaaggcac tttcccttct tgtcggtaat
     2101 ttcacaagtt gtcccatact tgcaagatcg cgcatcagct ccggtatacg tcccggtgaa
     2161 gcgccctgca atgtcatcag cattctcatc accattccgc atgattctga gaaactcagt
     2221 tgattcggcc agtaaccttt cagatgttcc gccattttaa tcatctgata tctcaccaga
     2281 ttataagcca gtaagacacc ccacagctct tgctccacaa gctccggctt tttacttctc
     2341 agcgtcagcc tgctcagttg catcgtctgt tttatctccc tgtatcccag ttcgatttcc
     2401 cagcgatgac tgtacagatc cgccatttct cctccgggga agcgcatggc gtccgtcatc
     2461 gacgtcagca gatggcagac ttttcctttg cgcgtcacgg tcagcaggcg ggctgtcact
     2521 tcatttccca gtcccggcca c
//
LOCUS       DAZKGF010000340         2505 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZKGF010000340 DAZKGF010000000
VERSION     DAZKGF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.341"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            234..1052
                     /locus_tag="ACHOQV_005095"
     CDS             234..1052
                     /locus_tag="ACHOQV_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9633313.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGEITSQHVPEIIL
                     LNSHDGTSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            1144..1629
                     /locus_tag="ACHOQV_005096"
     CDS             1144..1629
                     /locus_tag="ACHOQV_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9633314.1"
                     /translation="MTTSSHNSTAPSVSVTTASGNNQSQLVATLIPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDEKWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            1645..2121
                     /gene="radC"
                     /locus_tag="ACHOQV_005097"
     CDS             1645..2121
                     /gene="radC"
                     /locus_tag="ACHOQV_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9633315.1"
                     /translation="MQQLSFLPGEMTPGERNLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGRQVFSFAEHGLL"
     gene            2190..2411
                     /locus_tag="ACHOQV_005098"
     CDS             2190..2411
                     /locus_tag="ACHOQV_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9633316.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGSYVRLMSVTLN"
BASE COUNT          582 a          696 c          647 g          580 t
ORIGIN      
        1 atttaatttt gggggaagat acaaaaacaa ccagtgaaca tatttccggc gggttatccg
       61 caatgctgaa ggtcagttgc actggcgatg ccggaataca gcttaatacg tggttcgcca
      121 gtgaagggct tctcagacaa taaacctcat tcatttcacc catcaggccg cgtcttctcc
      181 gggagacgcg gctttttcat ttatatcgct taatcattta taaggagcaa atcatgagat
      241 tagccagtcg ttttggttat gcgaaccaga tacgtcgtga tcgtccgctg acacacgaag
      301 aactgatgcg ccatgtaccg agtatttttg gggaaaaccg acatacctct cgcagtgaac
      361 actatgcgta cattcccact atcaccgtcc tggaaaatct gcagcaggaa ggcttccagc
      421 cgttcttcgc ctgccagacc cgtgtgcgcg accagagccg ccgggaatat accaaacaca
      481 tgctgcgtct gcggcgggcc ggagagataa ccagtcagca tgtgcctgaa attattctgc
      541 tcaactccca tgacggtacc tccagctacc agatgttacc cggatatttt cgtgccattt
      601 gtaccaatgg actggtctgc ggtcagtctc tgggagaatt gcgtgttcca caccggggaa
      661 atgtggtgga gaaagttatt gaaggggctt acgaggtggt gggggttttt gaccggattg
      721 aggaaaagcg tgatgccatg cagtcgctga ttctgccgcc accggcacgc caggcgctgg
      781 cacaggcggc actgacttac cgttatggtg acgaacatca gcccgtcacc accgccgaca
      841 ttctgacgcc acgacgccgg gaggattacg gtaaggacct gtggagtgct tatcagacca
      901 tccaggagaa tatgctgaaa ggcgggattt ccggtcgcag tgcaaaagga aaacgtatcc
      961 acacccgtgc cattcacagc atcgacaccg atattaagct caaccgcgca ttatgggtga
     1021 tggcagaaac gctgctggag agtatgcgct gatgccgttt cactgaaagt gcagccctga
     1081 ctcacgtctg taccagtccc cgggcattct tccattcatt tgctttttat aaggaataat
     1141 ctcatgacaa cctcttcgca taattccacc gcaccttctg tttccgtaac aactgcatca
     1201 gggaataacc agtctcaact ggttgccacc ctcatccctg atgaacagcg cataagcttc
     1261 tggccgcaac attttggcct cattccacag tgggtgaccc tggagccccg tgtcttcggc
     1321 tggatggacc gtctttgcga agactactgc ggtggtatct ggaatctgta caccctgaac
     1381 aacggcgggg catttatggc acccgaaccg gatgacgatg atgacgaaaa atgggtactg
     1441 ttcaatgcca tgaacggtaa ccgcgctgaa atgagcccgg aagccgccgg cattgctgcc
     1501 tgtctgatga cgtacagcca tcatgcctgc cgtacggaat gttatgccat gacggtccat
     1561 tattaccggt tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc
     1621 atcgactgaa tggaggccgg gacaatgcag cagctttcct ttctgcccgg agagatgacg
     1681 cccggcgagc gcaacctcat tcagcgggcc ctgaaaaccc tggaccgcca tcttcatgaa
     1741 cccggcgtgg ccttcacctc cacccgtgcg gcacgggaat ggctgattct gaacatggcg
     1801 ggactggagc gtgaagaatt ccgggtgctg tatctgaaca accagaatca gctgattgcc
     1861 ggtgaaaccc tcttcaccgg caccatcaac cgcacggaag tccatccccg ggaagtgatt
     1921 aaacgcgccc tgtatcacaa tgccgctgcc gtggtgctgg cacacaatca cccgtccggt
     1981 gaagtcacac ccagtaaggc agaccggctt attacggagc gtctggtaca ggcactggcc
     2041 ctggtggata tccgggtacc ggaccatctg atagttggtg gcagacaggt tttctccttt
     2101 gccgaacatg gtctgcttta acccgtcacc gtcaccacca catcatacct gttttcgctt
     2161 ttatcttctg tcttcagagg tatcccatca tgagaatcat cacccgtggt gaagccatgc
     2221 gtattcacca acagcatcct gcatcccgtc tttttccgtt ctgtaccggt aagtatcgct
     2281 ggcacggtag cgctgaggcg tataccggtc gcgaagtaca ggatattccc ggcgttcttg
     2341 ccgtgtttgc agaacgccgt aaggacagtt ttggctcgta tgtccggctg atgagcgtca
     2401 ccctgaactg atgttccgtc acccggaaaa cgagtacgac cagatggact cagacgccgc
     2461 tgtggatgag gccattctcg tcaatgcata catcttcacc gaaac
//
LOCUS       DAZKGF010000341         2500 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZKGF010000341 DAZKGF010000000
VERSION     DAZKGF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.342"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..471
                     /locus_tag="ACHOQV_005099"
     CDS             <1..471
                     /locus_tag="ACHOQV_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP9633317.1"
                     /translation="KAGKKLKKDNPNYNGDLGFKIFETVHDFRAKDESELTLSNLSFF
                     DDAVLTPEQYDTLLTTWCVYDGSLLTTPIEDIDLNGYKAHLCDGRLYLIAPNFTSEAL
                     KALLQKLDADKDFAPNKVVFYGSNFESAKQMELNEALKSYANKKSIDLDLVVRN"
     gene            474..>2500
                     /locus_tag="ACHOQV_005100"
     CDS             474..>2500
                     /locus_tag="ACHOQV_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFP9633318.1"
                     /translation="MSKGFTFEKNLPHQKAGVDSVMNVFVSAIPHQTDHVAIRLLANP
                     ELNLSEQQYYNNIKNVQEFNGIEHSKDNYDAKSNVIDVSMETGTGKTYTYTKTIFDLN
                     KSFGINKFIIIVPTLSIKAGTVNFLKSDAIKEHFRDDYERELKTYVVESQKSSGKNTK
                     SYMPQAIHDFVEASNFNKKYIHILVINSGMINSKSLTDTYDVGLLDNQFDTPFSALSA
                     IKPFIIIDEPHKFPTGKKTWEHIQKFNAQYIIRYGATFSEGYKNLVYRLTAVDAFNED
                     LVKGIDAYIEDIVGDGNANLKLVKSDGKEATFELNENNNKKIFKLAKGESLSKTHSAI
                     HDLTLDALNKNTAVLSNGIELKIGSSINPYSYDQTLADNMMRKAVKEHFKLEKEYLTQ
                     RPRIKPLTLFFIDDIEGYRDGNDIAGSLKTKFEEYVLAEANELLKTEKDEFYRNYLEK
                     TVKDISSVHGGYFSKDNSDKDDKIEQEINEILHDKELLLSLENPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNEYMCRVKNRDFTLNYYVDFTEKDFV
                     DSLVKEVNDSSFKETVPGKFTQELKDKILSQYPELSSRTLMNEIFDAEIIDDNENFKD
                     SDAYSRLKAKYPAAFPAGVKPSKIKKATDGKKRTKMRIGKFSELKELWDLI"
BASE COUNT          867 a          422 c          476 g          735 t
ORIGIN      
        1 aaggcaggaa aaaagctgaa aaaagataat cctaattata atggagattt gggatttaaa
       61 atatttgaaa cagttcatga ttttcgagcg aaagatgagt ctgaacttac gctttctaat
      121 cttagtttct ttgacgatgc tgtattgaca ccagagcagt atgacactct attgacgaca
      181 tggtgcgtgt atgatggcag cttgctaaca acaccaattg aagatattga ccttaatggc
      241 tacaaagcac acttgtgcga tggtcggcta tatctaattg cgcctaattt cacaagtgag
      301 gctttgaaag ctttacttca aaagctggat gcagacaaag actttgcccc taacaaagtt
      361 gtattttatg gcagcaactt cgaaagtgcg aaacagatgg aactcaatga agcactaaaa
      421 agttatgcta ataaaaaatc tattgattta gatttggtgg taaggaacta atcatgtcta
      481 aagggttcac attcgaaaaa aatttacctc atcaaaaggc tggcgttgat tcggtaatga
      541 atgtctttgt atccgccata cctcatcaga cggatcatgt tgcaattcgc ctgttagcta
      601 atccagaact gaatctatct gaacaacaat actacaacaa cataaaaaat gttcaggagt
      661 tcaatggtat tgagcactca aaagataatt acgatgctaa aagcaacgta attgatgttt
      721 ctatggagac aggtacaggt aaaacttata cttacaccaa aacaattttt gacctgaaca
      781 aatcatttgg tattaacaaa tttatcatca ttgttcctac cctatcgatc aaagccggaa
      841 ccgttaactt tttaaaaagc gatgctataa aagaacactt tcgagatgat tacgagcgtg
      901 aactaaagac ttacgttgtt gaaagtcaga aaagttcggg taaaaataca aaatcgtata
      961 tgcctcaagc tatccatgat tttgttgaag caagtaattt caataagaaa tatatacaca
     1021 ttcttgtgat caactcaggg atgattaact ctaagtcttt aactgacact tatgatgttg
     1081 gcttgcttga taatcagttt gataccccat tttctgcact tagtgcgata aaaccattca
     1141 ttatcattga tgagcctcat aaatttccta ctggtaaaaa aacatgggaa catattcaaa
     1201 aatttaatgc ccaatatatc atccgctatg gcgcaacctt tagtgaaggc tataagaact
     1261 tagtttatcg tcttacagcc gtagatgcct tcaatgaaga tcttgttaaa ggtattgatg
     1321 catacattga ggatattgtt ggtgatggta atgccaacct caagttggtc aaatctgacg
     1381 gaaaagaagc tacatttgaa ctgaacgaaa ataataataa aaaaattttt aagttagcca
     1441 aaggtgaatc actatctaag acgcatagtg cgatccatga tttaactctt gatgctttaa
     1501 ataagaatac agcagtgttg agtaatggta ttgagctaaa aattggtagc tcgataaacc
     1561 cctattctta cgatcagaca cttgctgata acatgatgcg taaagctgtc aaagaacact
     1621 ttaagttaga aaaagagtat ctgacacaaa ggccacgcat aaaacctctt actcttttct
     1681 tcattgatga cattgaaggc tatcgtgatg gtaatgatat tgcaggaagc ctgaaaacta
     1741 agttcgaaga atacgttttg gcagaagcaa atgaactgtt aaaaacagag aaagatgagt
     1801 tttacagaaa ctaccttgaa aaaactgtta aggatatatc ttctgttcat ggtggttatt
     1861 tttcaaaaga taatagtgat aaagatgata aaattgagca ggaaatcaac gaaatccttc
     1921 atgataaaga gcttctattg tctttagaaa atcctcgccg tttcattttc tctaaatgga
     1981 cgttgcgtga gggatgggat aacccaaatg tctttcagat ttgtaagctc cgttcgagcg
     2041 gtagcaccac atctaaactt caggaggtag ggcgtggttt acgtcttcca gtgaatgaat
     2101 acatgtgccg tgtgaaaaac cgcgatttta ctctcaacta ttatgttgat tttacggaaa
     2161 aagattttgt tgattctctt gtaaaagagg ttaatgatag ttctttcaaa gaaactgttc
     2221 caggtaagtt tactcaagaa cttaaggata aaatcttatc tcagtatcct gagctttcat
     2281 ccagaacact catgaatgag atctttgatg ctgaaattat tgatgataat gaaaacttca
     2341 aagattccga tgcctatagc cgtttaaaag caaaatatcc ggctgcattc cctgccgggg
     2401 tgaaaccaag caaaataaaa aaagccacgg atggaaaaaa gcgtactaaa atgcgtattg
     2461 gtaagttcag tgagttgaaa gaactatggg atttgatcaa
//
LOCUS       DAZKGF010000342         2495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZKGF010000342 DAZKGF010000000
VERSION     DAZKGF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.343"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            134..1567
                     /locus_tag="ACHOQV_005101"
     CDS             134..1567
                     /locus_tag="ACHOQV_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP9633319.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVAISALTA
                     FSIACQNQIDVCRPGNLRGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKLYKEIRAGKDCKATESELKRHLNKCPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGIIFDGRAMSKLGILNSLWDGGSLFIDRKSS
                     PGIILKDPRLTVSVMIQPDVYQKGFCTRKKELVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEQRINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIHHSSGEEGDISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNPALTVPVRLIPEELELFNWIKSYCIEKGIPCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLYVAPNFDL"
BASE COUNT          710 a          437 c          567 g          781 t
ORIGIN      
        1 tcggtttatt aaacatcaga tctacaaatg ctctgtattt ctcagagcat caggtctgaa
       61 tagcagctat tttcgctatt gtagcgggag ttgctgctat tttacttacc cattaatgaa
      121 aagagaggct tttatgtgct tacttgcacc agaaaatcct tatccgatat atgcactacc
      181 accgctggtg agaaatgcaa taattgaaac tcaaaaaaat acacaggcac ctttggctat
      241 ggtggcgata tccgcattaa cagcgttctc aattgcctgt cagaatcaga ttgacgtgtg
      301 cagacctgga aatttacgtg ggcctgttaa tctttactct ctgattctgg ctgattccgg
      361 tgaaaggaaa acgaccgtgg ataaagtgtt tatgaaagca ttttatctca gggatgaagc
      421 cctggcggaa gaatacgcga aactggttga gaattacagt acagaaaagg aaatatggga
      481 gcaaaagcaa aaagcactgg agtcaaaatt gtataaagaa attcgcgccg gtaaagattg
      541 taaggcaaca gagtcagagc ttaaaagaca tctgaataaa tgtcctgttc cgccgcagat
      601 acgccggaca attttcaatg agacaacgat agagggaatg ttaaaatatt attccgacag
      661 caatcgctct tttgctcttg tatccagtga aggaggaata atttttgatg gccgggcaat
      721 gagtaaactt ggaatactta actctttgtg ggatggtggt tctcttttca tcgacaggaa
      781 atcatctccc ggaattattt tgaaggatcc aagactgacg gtatcggtga tgattcagcc
      841 tgatgtttac cagaaaggtt tttgtacgcg aaaaaaagaa cttgtgaaaa cgtcaggaca
      901 tcatgcaagg tttttgatgt gtcaaccaac atcaacgcag gggacaagga taataaccgg
      961 agataattat tcatcacagt atcaggattt gtttgaacag aggatcaatg agcttattga
     1021 tgaaagtctg gcgatgagtg gtgaacgacg ttgccttcac ttttctcctc aggctgccag
     1081 aatctggacg gattattaca acgatgtgga atcgaagctg gggggattgg ggcctttaag
     1141 acattgccgg gaatatgctg ctaaaaatgc agagtatatg gcaagactgg caggacttat
     1201 tcaccattcg agcggtgaag agggggacat ttccccttac actgcagaaa tggcgagaga
     1261 attagcaata tggtacggta atgagtatgt gcggttgtct aatccgttaa cttttgacaa
     1321 ccctgccctg accgtacctg tgcgactcat tccagaggag cttgaacttt tcaactggat
     1381 aaaaagctat tgcattgaga aggggatccc ctgtatgaaa aaaaatgaca ttttacagcg
     1441 tggtccgaac cgtttccgga aaaaggataa aatcaactgg ttactggatt tactgtatga
     1501 acaaaacaga gttgtaccgg ttattgaggg aaaaacgttg tatgtcgcac ctaactttga
     1561 cctctgaacg acttaatcgt tgagtaacat ctagtctcct ctgctacttg cggaggagac
     1621 taatgccttt accatgtcag cgttgttgta ttagaaaact ctggtacagg tcagtcagta
     1681 aatataactg gtgtaaccac tatttactat tggatagagt gttgctcagg atattgtcgg
     1741 ctttagtctg aggtgaggtg ggaccttcat tgttttcgtg ttcaataaat aatttaggtt
     1801 aattagtatt attaatggga gttatgttat ctgttatgtt gtcttatctc tttacatggt
     1861 aatatcactc ctctttatgc taagtattct gtatgtgcgt aatacatata tctccataca
     1921 ctacgcagtg aagcatatgg ggggaagctg tatggtgaat tatgtggtgt aatgttggga
     1981 acataaatct gttatgttgt cttgtacgca cttaacactg gttacgtacc ggaacactat
     2041 tagagtatat tgtctttagt tcgtacgtta tagctccgtt acagtttatg ggtaaatata
     2101 atcttttctg tatgaaataa tcttcttatt gtgaggttaa atatctgtag gctctctctg
     2161 cagagtctct gtttttactt aatggtacgg cgatactaat agcgatgttg cctgtggcta
     2221 ttcaatgctg acctttgtga agatcagatt atttcctgta agtaattgaa tggtgctttc
     2281 ttaaactcaa ttatatgagg tgtgtttttt attgtaattc gttctggtcc ttagtttaag
     2341 agagtcttac tgtattctga atcattaccc tattactgta atgaggtaat acgaaacagt
     2401 tttatcaggt tgtctgtgct gctggttacc tcgtttatta atgccgcggt acggcgtttc
     2461 tgtactctca gtatttatgt aattccactt tcctg
//
LOCUS       DAZKGF010000343         2461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZKGF010000343 DAZKGF010000000
VERSION     DAZKGF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.344"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..379)
                     /locus_tag="ACHOQV_005102"
     CDS             complement(<1..379)
                     /locus_tag="ACHOQV_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633320.1"
                     /translation="MTGLSEQAKELHMMMGRRKPPKIQAVLNTRYWKNTEKTIKRTVG
                     MNIHTLSQWCGEQYEKMFISKVAEVYMLHHTAAILRRKGVYVDVFKIPSKIMNPPEAR
                     ANCTGCPSSQNVSPFFAYSEPNTI"
     gene            493..1377
                     /locus_tag="ACHOQV_005103"
     CDS             493..1377
                     /locus_tag="ACHOQV_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP9633321.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICTTHPLNGCTRQAHRLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRHQELSLATVTVRVATLFPSSFPVLPVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGETIGKSFDVAVARFSFPAWMLQLLR
                     KARDRIIRLLVTLWDHLF"
     gene            1496..2173
                     /locus_tag="ACHOQV_005104"
     CDS             1496..2173
                     /locus_tag="ACHOQV_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633322.1"
                     /translation="MNTRILFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATSA
                     SVEFIGK"
     gene            2179..2417
                     /locus_tag="ACHOQV_005105"
                     /pseudo
     CDS             2179..2417
                     /locus_tag="ACHOQV_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
BASE COUNT          605 a          636 c          577 g          643 t
ORIGIN      
        1 ttatggtgtt aggctctgaa taagcaaaaa aaggagagac attctgagaa gaagggcaac
       61 cagtacaatt ggctctcgct tcaggcgggt tcattatttt tgagggaatc ttaaaaacat
      121 ccacataaac tcctttacgc ctcaatattg ctgccgtatg gtgaagcata tagacttctg
      181 ccacttttga aataaacatc ttctcatatt gttctccaca ccactgactg agcgtatgaa
      241 tattcatacc tacagttctt tttatcgttt tttctgtgtt tttccagtat cgggtgttaa
      301 gaacagcctg tatcttcggc ggttttctcc gccccatcat catatgaagt tcttttgcct
      361 gctcagataa tcctgtcatt tattacctcc gaatatattc cattaaaaat ggaaataatt
      421 atacgcccat attacctgaa tgataaaccg gattactgtg atccggttca gtgaaatcaa
      481 caggataccg gtatgtcatt cagccattct tccctctctg cacaagtgaa atcatatctg
      541 acgtttcttc ctgaagaaat tcgccagaaa atccttgaac atctccacgg tgttattcat
      601 tacgagcccg tgattggcat tatgggtaaa tccggcaccg gcaaaagcag tctgtgtaat
      661 gccatttttc agtcccgtat ctgcaccacg catcccctga acggctgcac ccgccaggct
      721 catcgcctca ccctgcagat tggtgaacgc agaatgacgc tggtcgatct gcccggcatt
      781 ggtgaaacac cgcagcatga tcaggaatac cgggagcttt accgtcagtt actgccggaa
      841 ctggatctga ttatctggat cctgcgggct gatgaacgtg cgtatgctgc cgatattgcc
      901 atgcatcagt ttttactgaa tgaaggtgca gatccctcgc gttttctgtt tgtactcagt
      961 catgccgatc gcgtgtttcc tgctgaagaa tggaacgaca cagaaaaatg cccgtcccgt
     1021 caccaggaac tctcactggc aactgttaca gtccgggtgg caaccctgtt cccgtcatca
     1081 tttccggtac tccccgttgc tgcgcctgtc ggctggaatc ttccggcgtt tgtgtcacta
     1141 atgatccacg ccctgccacc gcaggcaacc agtgcggttt attcacatat ccggggggaa
     1201 aaccgctctg aacaggcccg gaaacacgca caacagactt ttggtgaaac catcgggaaa
     1261 agttttgacg tcgccgttgc ccgtttcagt tttccggcct ggatgttaca gcttctgcgt
     1321 aaagcccggg accgcattat ccgcctgctg gtcacactgt gggatcacct gttctgagcg
     1381 ccccggagtc ccccgtggca accgtatacc tttacacgac aacggattac ggtaaccact
     1441 gccgtatacc gtctgtcatt atcaataaca cactctttaa tttcagaggt cagtcatgaa
     1501 taccagaatt ctttttcccc tgctgttcac tgtggcatcc ttctccgcct ccgccggtaa
     1561 ctgggctgtc aaaaacggct ggtgccagac catgacggaa gatggtcagg cgctggtaat
     1621 gctgaaaaat ggcacgattg gtattaccgg cctgatgcag ggatgcccga atggcgtaca
     1681 gacgctcctg ggcagccgta tcagtattaa cggtaacctg atccccacat cacaaatgtg
     1741 taatcagcag acgggattca gggctgttga ggtggaaatc ggacaggcgc cggaaatggt
     1801 caaaaaagcc gttcactcca tagcagagcg tgatgtgtcc gttttacagg catttggtgt
     1861 acgaatggaa ttcacccgcg gtgatatgct gaaggtctgt ccgaaatttg tcacatcact
     1921 tgccggtttt tccccgaaac agacgaccac tattaataaa gactccgtcc tgcaggctgc
     1981 ccggcaggca tacgcccggg aatatgacga ggaaacaaca gaaaccgctg attttggctc
     2041 ttacgaagta aaaggcaata aggttgagtt tgaagtattc aatcctgaag accgtgcgta
     2101 cgacaaagtg accgtcacgg ttggtgctga cggtaatgcc accagtgcca gcgttgaatt
     2161 tatcggaaaa tagccggtat gtcggactgt caccctgttt tattgcccga aggccctttc
     2221 tcacgcaaac aggcaatggc tgccacaaca gcttacctta ccgcaatgtg cttattgaag
     2281 atgaccatgg aacgcatttc ctgctggtta tccgcaatgc cgaagggcag ctacgctggc
     2341 ggtgctggaa ttttgagtct gacgccggaa aacagcttaa tgtgtggctc gccagtgaag
     2401 ggcttctcag acaataaaag tcttcatttc atccatcagg ccgcgtcttc tccgggagac
     2461 g
//
LOCUS       DAZKGF010000344         2416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZKGF010000344 DAZKGF010000000
VERSION     DAZKGF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.345"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..978
                     /locus_tag="ACHOQV_005106"
                     /pseudo
     CDS             1..978
                     /locus_tag="ACHOQV_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            1255..>2416
                     /locus_tag="ACHOQV_005107"
     CDS             1255..>2416
                     /locus_tag="ACHOQV_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9633323.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQ"
BASE COUNT          675 a          473 c          612 g          656 t
ORIGIN      
        1 ttggtaaata aagttagtga aaacacaaaa cagcgcaatt tgttttccaa aaaagtgact
       61 ttgtgcataa ctttgttttt tgttcttggg gggccgatag ttgctcacat attttctagc
      121 cattatgaga gctacgattt acacattgcc gagttaacca ataaaaatgg tcaggtggtt
      181 tggaaagcct catatgttac cataatgatt ttcatgtggt taaccttgct ttcggttaat
      241 ctatacttta atggtttacg ctgtgatatc tggaacgggg tgtcggtgat tgctttttgt
      301 gccgtgctgt acaatatctc tctgttattt atgagcaggt atagcgtgtc catttggtat
      361 attagtcgca ctattgaagt agtcagtaag ttaaccgttt tggtaatttt tatgtgccat
      421 atattcagtg cgctatgagt aacaaaggac attgcacatc gcgatccctt aaccaatata
      481 tttaacagaa attatttttt taatgaactg acggttcaat cagcatcggc caaaaaaaaa
      541 ccttattgcg tcatgattat ggatattgac cacttcaaaa aagtcaacga cacctgggga
      601 catccggttg gcgatcaagt gataaagaca gtggtgaata tcattgggaa aagcatacga
      661 ccagatgacc ttttagcgcg cgttggcggc gaagagtttg gcgtcttgct gacggagatc
      721 gatactgaat gtgcgaaagc tttagcggaa aggattcggg aaaatgttga acgtttaact
      781 ggcgataatc ctgaatacgc cataccacaa aaagtgacaa ttagtattgg cgctgttgta
      841 acgcagaaaa atgagttaaa ccctaaggag atctatcaac tggctgataa cgcactttac
      901 gaggcgaaag agaccgggcg taataaggtg gtagtaaaag aggcagaaat tcttatcaac
      961 cgcaaggatg atgaatgagt ctgtcgctag ttgtcgacct ccgcctgggg cttctcatcc
     1021 cccagtgtgt gaaatatacg aaaaaaagct cgcactttcg tacgagctct tctttaaata
     1081 tggcggtgag ggggaccgcc ttaaataaat ctgacttaat ccaggggggt tatataattt
     1141 cattataaac aatgcattac actttttgtt gtccgatatt atctaatgta ttcttcgtga
     1201 agcgagagac taatggcata ctggatggca taccagagtg acgggaatta gcgtatggca
     1261 gtattgacgg atacgaaagc aagacatatc aaacctgatg acaaaccatt gccccatggg
     1321 ggaattaccg gactgaccct tcatccttct tcagtaaagg ggcgggggaa atgggttttt
     1381 cgttatgtaa gtccggtgac acaaaaaaga cgtaatgctg gattgggaac ttacccagag
     1441 gtcagtattg ctgaagctgc acgtactgcc cggataatgc gagagcaact tgctgcaggt
     1501 gatgatcctc tggagattaa aaaggctgaa tctgagaaag tcgttatccc aacatttgcc
     1561 gatgcagcca ggcgtgtaca tgcagaactg tctcctggat gggaaaatcc aaagcatgta
     1621 aggcagtggt tatcgacgct tgagaattac gcgtttcctc aactgggagc aaaaacgctg
     1681 gattcgatta cggctgcgga cgtggcagaa acactgcgtc cagtctggtt aaccttgtca
     1741 gaaacggcaa gccgggttaa acagcgcatt catgttgtta tgcagtgggg atgggcgcac
     1801 ggtttttgtg tggcaaatcc tgttgatgtg gttgaccatt tgcttcctca gcagacaaga
     1861 ggacgtgatg aacaccaacc cgcaatgccc tggaggcagt taccgctttt tgtggcgacc
     1921 agtgtgtata gtgatgaacc ttataatgtt acccgcgcac tgttattaat ggtgatactt
     1981 acagcaactc gctcgggcga agctagggga atgcgctggg ctgaaattga ttttcataag
     2041 cgggtatgga ctatacccgc agaaagaatg aaagctaggc tacagcatcg tgttccttta
     2101 tcccggcagg ctatttacat tctggaaaat atacgtggcc tgcatgatga actggtgttc
     2161 ccttcaccca gaaagcagca gatcctttcc gatatggtgt tgacaagttt tctgcgtaaa
     2221 aagaaagccg tcagtgacat tccggggcga gttgccacgg cacatggttt tcgctcaaca
     2281 ttcagggact ggtgtagcga acaggggtat tcgcgggatc tggcggaaag ggcgctcgct
     2341 catacgctga aaaataaggt tgaggcggca tatcatcgta ctgatctact ggagcagcgt
     2401 gtaccgatga tgcagg
//
LOCUS       DAZKGF010000345         2379 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZKGF010000345 DAZKGF010000000
VERSION     DAZKGF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.346"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            172..2313
                     /locus_tag="ACHOQV_005108"
     CDS             172..2313
                     /locus_tag="ACHOQV_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFP9633324.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
BASE COUNT          655 a          563 c          558 g          603 t
ORIGIN      
        1 aacacatgta gcaatttttt gttatttctc atttactact acattactgc gtgattgaat
       61 gattctcaat tacatatccg atggcgaaat aacaaacgga tagtgcttag tgagcaataa
      121 gaaacaattt tcactcgcga caatccgcga gtttttggga taacagacta aatggctatg
      181 ttcacacctt cattctcagg actcaaaggt cgggcgctct tttcactgct ttttgcggca
      241 ccgatgattc atgcaacaga ctctgtaacg accaaagatg gcgaaacaat cactgttaca
      301 gcagatgcaa ataccgcaac tgaggcgacc gatggttatc aacctctgag cacttccacg
      361 gcgacattaa ccgatatgcc gatgctggat atcccgcagg tggtcaatac ggttagcgat
      421 caggttctgg aaaaccagaa tgcgacaacg ctggatgagg cgctttataa cgtcagtaac
      481 gtggtacaga ccaatacatt aggcgggact caggatgcct ttgtacgccg tgggtttggc
      541 gcaaaccggg atggctccat catgaccaac ggtctgcgaa ccgtacttcc tcgcagtttc
      601 aacgccgcca cagaacgtgt ggaagtgcta aaaggcccgg cctccacgct gtatggcatt
      661 ctcgatcctg gcggactgat taacgtcgtg accaagcgcc cggaaaaaac attccatggt
      721 tcggtttcag ccacctcctc cagttttggt ggcggcactg ggcaacttga tatcacaggt
      781 cccattgaag gcactcagct ggcgtatcgc cttaccgggg aagtgcagga tgaagattac
      841 tggcgaaact tcggtaaaga gcgcagtaca tttattgccc cgtcactcac ctggtttggt
      901 gataatgcaa cagtaaccat gctctattcc catcgggact ataaaactcc gttcgatcgt
      961 ggaacgattt tcgaccttac gacgaaacag cccgtaaacg ttgatcgaaa aatacgtttt
     1021 gacgaaccgt ttaatattac agatggtcag tccgatctgg cgcaactcaa cgcagaatat
     1081 catctcaata gccagtggac agcgcgcttt gattacagct acagccagga taaatacagc
     1141 gataatcagg cgcgtgttac cgcgtatgat gcaacgacag gaacactgac acggcgtgtt
     1201 gatgcaactc agggatctac ccagcgtatg catgctactc gtgcggatct gcaagggaat
     1261 gttgatattg ccggattcta taatgagatt ctgggtgggg tgtcatatga atattatgat
     1321 cttctgcgta cagatatgat tcgctgtaaa aaagctaaag atttcaatat ctacaaccct
     1381 gtttatggta ataccagcaa atgtacaacg gtttcggcgt cggacagtga tcagacgatc
     1441 aaacaggaga gctactcagc gtatgcacag gacgcactct atctgaccga taactggatt
     1501 gccgtcgccg ggatccgcta tcagtattac acgcagtatg cgggtaaagg ccgtcctttt
     1561 aatgtcaata ctgacagccg cgatgaacaa tggactccca aactggggtt ggtctacaaa
     1621 ctgacgccat cggtatcctt atttgccaat tattcgcaaa catttatgcc gcagtcgtca
     1681 attgccagct acattggcga tcttccaccg gaatcatcta atgcttacga agtcggggca
     1741 aaattcgagc tattcgatgg tatcaccgca gatattgcgc tgtttgatat ccataaacgt
     1801 aatgtgttgt ataccgaaag tgttggtgat gaaaccattg ccaaaacggc aggccgtgtt
     1861 cgttcaagag gggtagaagt cgaccttgcg ggagcattaa ctgaaaacat taatatcatt
     1921 gccagctacg gctataccga tgctaaggtt ctggaagatc ctgattatgc aggtaaacca
     1981 ttgccgaatg ttcctcgtca taccggttcg ctattcctga cctatgacat tcataacatg
     2041 cctggcaata acacactgac gtttggcggt ggtggacatg gcgtaagccg tcgttcggca
     2101 accaatgggg ctgactatta tctgccaggc tattttgttg ccgatgcctt cgccgcatac
     2161 aaaatgaaat tgcagtatcc ggtcacactg caattaaacg tcaaaaacct gtttgataaa
     2221 acgtattaca cctcttccat cgccacaaat aatctgggga atcagattgg cgatccgcgt
     2281 gaagtgcaat tcacggtgaa aatggaattt tgatcaaaaa agcccggtat gtttaccggg
     2341 ctgagactga tgacaaactc caaattgtct gatgcgcta
//
LOCUS       DAZKGF010000346         2364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZKGF010000346 DAZKGF010000000
VERSION     DAZKGF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.347"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..463)
                     /locus_tag="ACHOQV_005109"
                     /pseudo
     CDS             complement(1..463)
                     /locus_tag="ACHOQV_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(542..1726)
                     /locus_tag="ACHOQV_005110"
                     /pseudo
     CDS             complement(542..1726)
                     /locus_tag="ACHOQV_005110"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
     gene            complement(1742..2269)
                     /locus_tag="ACHOQV_005111"
     CDS             complement(1742..2269)
                     /locus_tag="ACHOQV_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFP9633325.1"
                     /translation="MRNGKYDVLSPLYSGEPVNEAEVLGAAVWLWMHSPLHRDAPLHT
                     LPDLLLPVIKHRQYVVATEQGRPVFFMSQAWLSPEAEARYLTQPAILMPQSDWNSGDR
                     MWVCDWVAPFGHTPDMSRLVRQDIFPRLCWRSLYHRGQQTGLRVMNFRGAQVSREEAR
                     QWRVQHPLAVDVPER"
BASE COUNT          499 a          764 c          584 g          517 t
ORIGIN      
        1 tgctggcaag cagacgtccc tgctggttat ccacggcacc ggttgtcttc acctgcagac
       61 tgcctgactg cgccgcctgc actttcccct tgcgattatc aaggctcagt gcatccagac
      121 gcaccgcgcc atttgccgca agcagccctt cccggttatc cacgcttccc ggcagactca
      181 gcgaaaaatc ccctgttccc gtctgaatca gactgccttt acgatttgac aggctttgtg
      241 ccgatatctg cagtgtgtcg gcatttatcg taccgccatt attgctgaac tgcccggcag
      301 tccgggcgga aagcgtaccg gcactcagtt gcgcattctg tgtggacaga tttccgctgc
      361 ctgcatccag cccggtatgt tgtccctgta tccggctgtt gcctaaatcc agcccctgcc
      421 ccttcagttg catgtcccca ccggacagaa cctgtccgtg tgccgccagt tcgccggtgg
      481 tactgacagt caggttgccc gctgccgcca gacgtccgtc atctttcacc ccggcggcca
      541 gcagactccg ctcaccgccg atcagacgcg ctgtctgaat ccgggtgttg cgacgtgatg
      601 ccacagagcc tgtatgggtg aatacaccat cactctgtat ctgcgtatcc tgccccgcat
      661 aaacagaacc actgttatgc agctcccggg ttgtcgccag atgtacatcc gttttcgcac
      721 tgatactgcc actgttttca attcgtccgt cagccgtcag ggtaaccgcc cctgcctgtg
      781 caccaatatg accggcgtta cgaacaccca cccccgtttc cgtaccgaca aggcgaattt
      841 ttccggcata cattccgcca aggctggaca catccagcgc cacctgtggc tgcgaagagg
      901 catcagcggc ttttttctca gtctgcccgt gtgcaatatc cacattgtta cgtccggtgg
      961 tcactttcag gtcctgcgcc cagataccgg cattaatctt cacagaccgg gcaatgatat
     1021 cggtataatc cgtgcgcgag gcatccatcc ctttgcccgt aatctgaatc tcaccacatt
     1081 caacactgaa tccggtcaga ctgccgtttt tcatctgcgg ctgccccgtg gtcagcgtgg
     1141 cccggttcgc attaataaaa ccgcacccgt cgcaactgat gccggccggg ttcgcaatca
     1201 ccacctgcgc ctttttcccg gcaacctcaa caaaaccgtt caactggctg ggattgcgac
     1261 tgttcacttc attaagaatg attctggcct ctcccctggc cagccacggg ttcccggcca
     1321 ccatgccacc cagctgagtc tgcacattgt tatgggcatt attcagaatg acgccctggt
     1381 taccgacgtc gaactggctg tatgtattat gtgaaacccc gccggcagaa ggcgtctgaa
     1441 tattcacctg cggaatgccg ctggccgtct gcgtaatcgt gggctgctga tgccccggtg
     1501 cgccatggtc tgcgacaatg cctgcagcac tgacagaatg caccatcccc gacgccagcc
     1561 acaaagagaa tgcaagcggc gtgacacggc agatacgttg cctgtgtgga tatcctgtgc
     1621 gggaggacag tgatgtaccg gcgcgaccgg aacgggcaat atctgccacc accatcagca
     1681 ttccccgggc tttgttaaaa ataatgcggt aacaatttct gttcattctg ataaacctga
     1741 cttatctctc cggcacatcc acagcaagcg ggtgctgcac acgccactga cgcgcctctt
     1801 cacgactgac ctgtgcaccc cggaaattca tcacccgcaa cccggtctgc tgcccacggt
     1861 ggtacagcga acgccagcac agacggggaa aaatatcctg ccggacaaga cggctcatat
     1921 ccggagtgtg tccaaaaggg gcaacccagt cacagaccca catccggtcg ccgctgttcc
     1981 agtccgactg aggcatgaga attgccggct gcgtaagata acgcgcctct gcctccgggc
     2041 tgagccaggc ctggctcatg aaaaaaaccg gtcgtccctg ctctgttgcc accacatact
     2101 gtcggtgctt aatcaccggc agcagcaggt ccggcagggt atgcaatggc gcatcacgat
     2161 gcagggggga gtgcatccac agccagacgg ccgcccccag aacttccgcc tcattgaccg
     2221 gctcaccgga atacagggga ctcagaacgt catatttccc gttacgcatg aaattttctc
     2281 agaaaccccg gaacagatta aaaccggctg tcacatcact cgtcataaaa cccgccggtt
     2341 ttgaaaacgg acgaccgatg aaaa
//
LOCUS       DAZKGF010000347         2341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZKGF010000347 DAZKGF010000000
VERSION     DAZKGF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.348"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            216..782
                     /locus_tag="ACHOQV_005112"
     CDS             216..782
                     /locus_tag="ACHOQV_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP9633326.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMLARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHAVFWIDRQRVKKIY
                     PFAERIAECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCSEVPERPAAGRPRNLQK"
     gene            1040..1615
                     /locus_tag="ACHOQV_005113"
     CDS             1040..1615
                     /locus_tag="ACHOQV_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633327.1"
                     /translation="MNAIPYFDYSLAPFWPSYQSKVIGVLERALREQSGSRIRRILLR
                     LPCEYDNTFNSRTTWFGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVLLDSCSPDCEDILKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPGYPGSAGLF"
     gene            1684..1920
                     /locus_tag="ACHOQV_005114"
     CDS             1684..1920
                     /locus_tag="ACHOQV_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9633328.1"
                     /translation="MLTSITGHDSILLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            2202..>2341
                     /locus_tag="ACHOQV_005115"
     CDS             2202..>2341
                     /locus_tag="ACHOQV_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633329.1"
                     /translation="MQITKALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQ"
BASE COUNT          569 a          596 c          549 g          627 t
ORIGIN      
        1 cctcgtttat ctcatctctt tagctaatac catcagataa tccatttctt tctgcataat
       61 gctgcgtatc gttaataacc cgtcgtatcc attctgctac agcatgcctg ataaatacca
      121 tctgtaagtt attaccgttt tagatctgat tatgagcgaa agcattaatt cgttcacaga
      181 gcttaaaaca tcattaactt tcaggagtca tcaacatgcc taaatcttac acaccaaact
      241 ggttttttac cgctttactt gacaatcaca tcaatcaaat gctggcacgt tattcctgcc
      301 tgcgagcttt acggatggat ttcttctaca ggaaagatac gcctgatttc ttacaacctg
      361 atcatcgctg gcttgaattg cagttgcgta tgctgctgga gcaggtggaa caatttgaaa
      421 atatcgttgg cttcttctgg gtgattgaat ggacggctga tcatggtttt catgcacatg
      481 cggttttctg gatcgatcgt cagagggtta aaaaaatata tccctttgcg gagcggatag
      541 ctgaatgctg gcggtctatt acgcataaca gcggttcggc acaccgctgt acatatcagc
      601 cgcattatac atacaacatc aacattcctg tgcgccacaa cgatcctgaa agcatcgata
      661 atattcgcgg tgccctgcat tatctggcga aagaagaaca aaaagacggg ctgtgtgcct
      721 acggctgcag tgaagttcct gagcgtcctg ccgcagggcg tccacgtaat cttcagaagt
      781 gaagcttaaa ccttgagttt cctggcccgg aaacactccg ttagtaaaaa cttaccgccc
      841 tgcttaatga tgtgaaccga agtcaatgga gatcattcac cctgaaccac atccggtgtt
      901 ttgttccttg tcagttacct gatcttcccg ttttgccccg gtttttcact tattccatca
      961 accccatccg caaaccataa cacgtcagct tattcacggg caggacgcat tgtgggctgc
     1021 gcataatgga gcatatctta tgaatgctat cccttatttc gactatagcc tggcaccctt
     1081 ctggccatct tatcagagca aagtcatcgg cgttcttgag cgtgcgctgc gtgagcagtc
     1141 cggctcacgg atacggcgga tcctgcttcg tctgccgtgt gaatatgaca ataccttcaa
     1201 cagcagaacg acctggtttg gtatggattt tatcgaaacc gtcagtgcgc tgatgaatgc
     1261 gaaacccgga cgcgaccttt gctggctcct gacccgtcat cctgaaaagc cggaatacca
     1321 cgtggtgctg tgtgtcagac aggagtattt cgacggccct gaactggatc ggctaattct
     1381 ggatgcctgg agcaacgtac tgggcttcgc gtcaccgggt gaagcagcac cgtaccagaa
     1441 gcagatcacc agggatgtac tgctggacag ttgttcaccg gactgcgaag acatcctcaa
     1501 agacttgatc tgggcgttca gtgatttcgc ccgcgatcgt cgtggtgtgt gcgatccgga
     1561 agcccgttgc ctcgccggta atcccggcta tcccggatct gcaggactgt tctgaaagct
     1621 cagttcagca gattcaccag catttgccca acctccataa atctcacaga ggacatcctg
     1681 actatgttga cctcaataac aggccacgac agtatattgc tgcgtgccga cgatcccctg
     1741 atcgacatga actacatcac cagtttcacc ggtatgaccg ataaatggtt ttacaagctg
     1801 atcagtgaag gtcattttcc taaacccata aaactgggtc gcagcagccg ttggtacaaa
     1861 agtgaagtgg agcagtggat gcagcagcga attgaggaat cacgaggagc agctgcatga
     1921 aacgtgttgt gatgccggta cgctggcaat gtgcaaaatg tcggcgctgg tattgtggag
     1981 atcagccctg ctcctggtgc tggcgctgac aactcccgct catctttctg ctgactcctt
     2041 tggtcagcca actgtttgtc atcatttcct gactgattcg tcattccctt cgtcccgcca
     2101 gtcactggtg tttgtatcgt atcgtgccgg tgttgatgtg cgctttccgt gtgtctgcac
     2161 cggcttaaca aaattcaaca gggtttgaaa aggaacattt cgtgcaaata accaaagcct
     2221 taatttcaga gccgggagac atccggcgtt ttgttcagca ggcggtggac cactggccaa
     2281 atctgttggc atttcacttc acgctctatt cggcagaagg aaacatcaac gggcaacaga
     2341 t
//
LOCUS       DAZKGF010000348         2331 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZKGF010000348 DAZKGF010000000
VERSION     DAZKGF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.349"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2076
                     /locus_tag="ACHOQV_005116"
     CDS             <1..2076
                     /locus_tag="ACHOQV_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFP9633330.1"
                     /translation="RLTIISGRDTTLTGAQAGGETVKVDAGRHLTLTSEQDSDRYDSK
                     QQNASAGGSFTFGSMSGSASVNLSRDKMHSNYDSVQEQTGIFAGRGGFDVTTGQHTQL
                     NGAVIASTATADKNRLDTGTLGFSDIKNRADFKTEHQSTGLSTGGSVAGNFLGNMANN
                     LLVGANHEGHADSTTQSAVSAGNITIRDTQSQKQDVADLNCDAAHANQTLSPIFDREK
                     EQQRLQQAQLIGEIGNQVADIARTEGQIAGEKAKRDPAALNQARAELEAAGKPYTEQD
                     VAQRAYNNGMAASGFGTGGKYQQAIQAATAAVQGLAGGNLSAALAGGAAPYLAEVVKT
                     MTTDPVTGEVNKAANVTAHAVVNAALAVAQGNNALAGAAGAATGEVVGMIATQMYGKP
                     VSELSEAEKQTVSTLATVAAGLAGGLVGDSGGAAVAGAQSGKTTVENNYLSSKQIDAW
                     SAEMKSCQAKGGDCGGIIKKYEELSTAQQKQLISDCAASPATCQQKYGDVLTDSLAVK
                     QAIDRALGEDIPIKMVYDLTATFAQQMQAEGVVATNKVSEALQEEYGLDEVQAGIVAS
                     AAASAFGGISKVKGNSQPKIEQILKPEKNWETARNKALDLVGNLGADSKPVIGRLEVS
                     AGNGKVIGRQSSDGKVGWRVDYDPEKGTHINIWDYSQGKGPGKAVKQVIPFEGNEKSF
                     ETILKQLNR"
     gene            2082..>2331
                     /locus_tag="ACHOQV_005117"
     CDS             2082..>2331
                     /locus_tag="ACHOQV_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633331.1"
                     /translation="MTLFDECREALSADFNIVEGLAQQEALGILNKYPLAKGSVTWAE
                     IWHSDYESFDELLCANSVKNDDMFVFADDASIPVFRSNL"
BASE COUNT          648 a          503 c          726 g          454 t
ORIGIN      
        1 cggctgacca ttatcagcgg gcgggacacc acgcttaccg gcgcgcaggc tggcggtgaa
       61 acggtgaaag tggatgccgg tcgtcatctg acactgacca gtgagcagga cagtgaccgt
      121 tatgacagta agcagcagaa tgccagcgcc ggaggcagct tcactttcgg gtccatgagc
      181 ggttcggcga gtgtgaacct cagccgggat aaaatgcaca gtaactacga cagtgtacag
      241 gagcagacgg ggatttttgc cggcaggggc gggtttgatg tcacgaccgg acagcataca
      301 caactgaacg gtgccgtgat tgcgtccacg gcaacggcag ataaaaacag gctggatacc
      361 ggcacactgg gcttcagtga cattaaaaac cgggctgact ttaaaacgga acatcagagt
      421 acagggctca gtaccggtgg cagtgttgcc ggaaatttcc tggggaacat ggcaaacaat
      481 ctgctggtcg gtgcaaatca tgaaggccat gcggacagta ccacgcagtc ggcagtgtct
      541 gccggaaata tcaccatccg ggatacgcag agccagaaac aggatgtggc agacctgaac
      601 tgtgacgcag cacatgcaaa tcagacgctg tcccccatct ttgacaggga aaaagagcag
      661 cagcgactgc agcaggcgca actcattggt gagattggta accaggtggc agatattgcc
      721 agaacggaag gacagatagc cggtgagaaa gcgaagcggg acccggcagc actgaatcag
      781 gcccgtgcag agcttgaggc agcaggaaaa ccgtacacgg aacaggatgt ggcgcagcgg
      841 gcctacaata acggtatggc ggcctcaggt tttggaacgg gaggtaaata ccagcaggcg
      901 atacaggccg caacggcagc agtacagggg ctggccggcg gtaatctgag tgcagccctg
      961 gcaggtggtg cagcaccgta tctggctgaa gtagtgaaaa ccatgaccac agacccggtt
     1021 acaggtgagg tgaataaggc tgcaaatgtg accgcccatg ccgtggtgaa tgccgcactg
     1081 gcggtggcac agggaaataa tgctcttgcc ggtgctgcag gtgcagccac cggcgaggtg
     1141 gtgggaatga tagccacaca gatgtacggt aagccggtga gtgaactgag cgaggcagag
     1201 aagcagacgg tctcgacact ggcaacggtt gctgccggct tggccggtgg cctggtgggc
     1261 gactcagggg gggcagctgt agccggcgca cagtcaggta agacgactgt tgagaataac
     1321 tatctgagca gtaagcagat agatgcttgg tccgctgaaa tgaagtcctg tcaggcgaaa
     1381 ggcggtgact gcggcggtat tatcaaaaag tacgaagagt tgagtacggc acagcaaaag
     1441 caactgatca gtgactgtgc agccagcccg gcaacctgcc agcagaaata cggtgatgta
     1501 ctgactgaca gtctggcggt gaagcaggca atagatcgtg ctctgggtga agatatcccg
     1561 atcaaaatgg tctacgatct gacggcgact tttgcgcagc aaatgcaggc tgaaggggtt
     1621 gtcgcgacca acaaagtctc ggaagcactc caagaagaat acggtttgga tgaagttcag
     1681 gcgggaatag tcgcgagcgc tgcggcgtca gcatttgggg gaattagtaa ggttaaaggg
     1741 aattcccagc ctaaaattga acaaatcctc aagccagaga aaaactggga aactgctcga
     1801 aacaaggcat tggatcttgt aggtaattta ggagcggact ctaagccagt tatcggacgt
     1861 ttggaagtca gcgctggtaa tgggaaagtc atagggcggc aatccagcga cggaaaagtt
     1921 ggttggcgtg ttgattatga tcctgaaaaa ggcacacaca tcaatatatg ggattactcg
     1981 cagggtaaag gccctggaaa agcggtgaag caagtcatac cttttgaagg caatgaaaag
     2041 tcttttgaaa caatattgaa acagctaaac aggtagagca aatgacttta tttgatgagt
     2101 gtcgggaagc actgagtgct gattttaata tagtagaggg gctggctcag caggaggctc
     2161 tgggcatttt aaataagtac cctctcgcga aaggaagtgt tacttgggca gagatctggc
     2221 attctgatta tgagagtttt gatgagctat tgtgtgcaaa ctctgtaaaa aacgatgaca
     2281 tgtttgtctt tgcagatgat gcaagtattc cagtttttag gtcaaatcta a
//
LOCUS       DAZKGF010000349         2310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZKGF010000349 DAZKGF010000000
VERSION     DAZKGF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.350"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..1207
                     /gene="yhiN"
                     /locus_tag="ACHOQV_005118"
     CDS             14..1207
                     /gene="yhiN"
                     /locus_tag="ACHOQV_005118"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9633332.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGITWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(1294..>2310)
                     /gene="yhiM"
                     /locus_tag="ACHOQV_005119"
     CDS             complement(1294..>2310)
                     /gene="yhiM"
                     /locus_tag="ACHOQV_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFP9633333.1"
                     /translation="PLLMGIICIALGEFVLTGSGQSEYFVAGHVLISLSAICLALFTT
                     AFIIISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMADEFVAGHVIFGV
                     GMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLIAVPVLLTLFGFI
                     WSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTFSVKEHWLWCYWV
                     ILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWLLALVWRRTCSLA
                     NRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCFTLFSIVSILEAG
                     SAKK"
BASE COUNT          657 a          551 c          594 g          508 t
ORIGIN      
        1 agaggagcgc aaagtggaaa ggtttgatgc cattattata ggcgctggtg cggcgggtat
       61 gttctgttct gcgctggcag gtcaggcagg acgccgggtt ctgctgatcg ataatggtaa
      121 aaaaccaggg cgcaaaatcc tcatgtctgg cggtgggcgc tgcaacttta ccaaccttta
      181 tgtcgaaccg ggcgcttatc tgagccagaa tccgcatttt tgtaagtctg cactcgcgcg
      241 ttttacccag tgggatttca ttgatctggt caataaacac ggcatcacct ggcacgagaa
      301 aacgttaggg caactcttct gcgatgactc cgcgcagcag attgtcgaca tgctggtgga
      361 tgagtgcgag aagggcaatg tgaccttcag attgcgtagc gaagtgctga gtgtggcgaa
      421 ggatgaaaca ggcttcacgc ttgaactgaa cggcatgact gtcggttgcg aaaagctggt
      481 catcgcgacc ggtggactgt caatgccagg gctgggcgcg tcgccgtttg gttataagat
      541 tgccgaacaa tttggcctca acgtgctgcc gacccgcgcg ggcctggtgc cattcactct
      601 gcataaaccg ttgctcgaag agttacaggt gctggcgggc gtggcggtgc cttccgtgat
      661 taccgctgaa aacggcatcg ttttccgtga gaacttactc ttcacccacc gcggcttatc
      721 tggaccggcg gtgttgcaaa tttccagcta ctggcaaccg ggtgaatttg tcagtatcaa
      781 tctgctaccg gatgtggacc tcgaaacctt cctgaatgag cagcgtaatg cacatccgaa
      841 tcagagcctg aaaaacacac tggcggttca tctaccgaag cggttggttg aacgtttaca
      901 acaactcggg caaatcccgg atgtttcgct aaaacagctc aacgtgcgtg accaacaggc
      961 actgattagc acattgaccg actggcgcgt acaacccaac ggcactgaag gctatcgcac
     1021 tgccgaagtg acgctcggcg gcgtggacac caacgaactc tcttcacgga cgatggaagc
     1081 gcgcaaagtg cctgggctgt acttcatcgg cgaagtgatg gacgtcaccg gctggctggg
     1141 gggctataac ttccagtggg cgtggtcgag tgcatgggct tgtgcgcagg atttggtggc
     1201 agaataagaa gcaaaatgtg ctctcgatac cagagggatt aacctgaata aaaccagata
     1261 aggcgtccaa cgccttatct gaagcctttt ttattatttt ttcgctgaac ccgcttctaa
     1321 tatagaaacg atggaaaaca gtgtaaaaca aactgcacct aagccaacca gaactcgcga
     1381 aggaataaag taagccatat cggtttgcgc aatttcagcc aggaacgcag caaggaacag
     1441 acaaaacaga caggtaaata ccggaatcat gggtattctg ttggctaacg aacaggtgcg
     1501 tcgccatacc agcgctaaca accacacttt ggagaaaata ctgtagcaga tcatccccag
     1561 gcaaatgaga ataatgccgg gggccaggcg ggcgcttgca tctgaactga ttaatacgta
     1621 gataccaaaa ataatggtaa gtgagccaag caaaataacc caatagcacc acagccaatg
     1681 ttctttcact gagaacgtat tccgcgtttg atggacaata gtggcgacaa ggccaataag
     1741 acaagcgcaa attgccgtta accccattag tacgtgacct gcaacataat gtggtgtgat
     1801 atctgcacta cgtaacagta tgactgacca aatgaaacca aagagagtaa gcagaacggg
     1861 aacggcaatt aagcaattac cgattgttga agaataggcc tgcaatggcg taccgtcact
     1921 cttactgccc gaggcgtttt tggggatcaa cagaaaatgt ccggaagatg ccgctacggt
     1981 tgatacacaa gcggcaatca tgccaacgcc gaaaataaca tgaccggcga caaattcatc
     2041 tgccatcaca ttattgcttg ccaacagtga ccatccccaa atcatagttg tagcagaacc
     2101 ggcataacca ataacgggaa ataacctgtt gtagaattta ttcatgccgt gagttaactg
     2161 agatataata ataaatgcgg tagtgaacaa tgccagacat atcgcagaaa gagaaatcag
     2221 aacatgccct gcaacgaaat attcactttg cccagaaccg gttaatacaa actcacctaa
     2281 tgcgatgcat attataccca tcagtaaagg
//
LOCUS       DAZKGF010000350         2288 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZKGF010000350 DAZKGF010000000
VERSION     DAZKGF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.351"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            121..624
                     /locus_tag="ACHOQV_005120"
     CDS             121..624
                     /locus_tag="ACHOQV_005120"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9633334.1"
                     /translation="MNNIAPQSPVIRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(670..1086)
                     /gene="rraB"
                     /locus_tag="ACHOQV_005121"
     CDS             complement(670..1086)
                     /gene="rraB"
                     /locus_tag="ACHOQV_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFP9633335.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1248..2261
                     /gene="argF"
                     /locus_tag="ACHOQV_005122"
     CDS             1248..2261
                     /gene="argF"
                     /locus_tag="ACHOQV_005122"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP9633336.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          596 a          535 c          563 g          594 t
ORIGIN      
        1 caatactctt atcagtaata ggctggcaaa aactttattg ctttataaga cgcttgcgca
       61 cgtaagaaga gagtggtatt aaacaaatgt ataattactt tatttttatt gggttaaact
      121 atgaataaca ttgcgccgca atcacctgta atacgtcgcc tgacgctgca ggataatcct
      181 gctatcgccc gcgtcattcg tcaggtatcc gccgaatacg gtcttaccgc tgataaaggc
      241 tacaccgtcg ccgatccgaa tcttgacgag ctgtatcaag tgtatagtca gcctggccat
      301 gcatattggg tggttgagta cgagggtgaa gtggtcggcg gcggtgggat agcgccatta
      361 acaggtagtg aagcggacat ttgcgaactg cagaaaatgt actttcttcc cgccattcgc
      421 ggtaaagggc tggcaaaaaa actggcctta atggcgatgg agcaggcgcg agaaatgggt
      481 ttcaaacgct gctatctgga aacgaccgct tttttgaaag aagcgattgg tttatatgaa
      541 catttaggtt ttcaacatat cgactatgca cttggctgca cgggccatgt tgattgtgaa
      601 gtgcggatgt tgcgcgaact ctgaaaaaat gcccgctagt atgtgctatc gggcatttta
      661 atcaattaat tagtggcgaa ccccgtcatc gtcttcatcg acaaaatctt catcgtcgcc
      721 atcttcgccg ttaggatctt caaagtaagt gccccaaccg tcgtactcaa cgtcaaattt
      781 ctccgccagc gtcatcagtt gttcaacctg ggcatcgatc agatcggcat tcaacgcgca
      841 ctcgctgagg atgtcgcagc aaatcacgat atcaccatct tcaacttcca gctcttctgg
      901 atcggtcact tcgtaaccga gtttaaacgc ttcaactgcc gctttttcca gagtttccag
      961 atcgtctgcg gaaagatggt gttcgatggt gtacagcgcg tccggatcgc tgccatcttc
     1021 cagtaattct tcaataatca aacgtgtttc ttcacgctgt tcttccagtt gttccgggtt
     1081 tgccatggct cattcctctt aatgtcctgc cgatactctt attgtcacac accactgaca
     1141 ttgcctccac ctttgtgata aagatttatg ctttagactt gcaaatgaat aatcatccat
     1201 ataaattgaa ttttaattca ttgaggcgtt agccacagga gggatctatg tccgggtttt
     1261 atcataagca tttcctgaaa ttactcgatt tcacgccagc tgaacttaac agcctgctgc
     1321 agttagccgc gaagctgaaa gctgataaga aaagcggtaa agaagaagcc aaactcactg
     1381 gtaaaaacat cgcgctcatc ttcgaaaaag actcgacccg tacccgatgc tctttcgaag
     1441 ttgccgcata tgaccagggt gctcgcgtta cttatctcgg cccaagcggt agtcagattg
     1501 gtcataaaga gtcgattaaa gacactgccc gcgtgcttgg tcgcatgtat gacggtattc
     1561 agtatcgcgg ctatggtcag gagattgtcg aaacactggc ggaatacgct ggcgtgccgg
     1621 tatggaacgg cctgaccaat gagtttcacc ccacgcagtt gctggcggat ctcctcacca
     1681 tgcaggagca tttacccggt aaagcattca acgaaatgac gctggtctat gcaggtgacg
     1741 cacgtaacaa catgggcaat tcgatgctcg aagctgcggc gcttaccggt ctggatttgc
     1801 gtctggtcgc gccacaggca tgctggccag aagctgcgct ggttacggaa tgccgcgccc
     1861 tggcacaaca aaatggcggg aatattacgc tgacggaaga tgtagctaaa ggagttgaag
     1921 gtgctgactt tatctatacc gatgtatggg tgtcgatggg tgaagcaaaa gaaaaatggg
     1981 ctgaacgcat tgcattactg cgtgattatc aggtgaacag caagatgatg cagttgaccg
     2041 gtaacccgga ggtcaaattc ctccactgcc tgcccgcgtt tcatgacgac cagacgacgc
     2101 ttggtaagaa aatggcggaa gagtttggcc tacatggcgg aatggaagtg actgatgagg
     2161 tcttcgaatc tgccgccagc attgtttttg atcaggcgga aaaccgcatg cataccatca
     2221 aagcggtgat ggtcgcgacg ctcagtaaat taaataatta atatctctat aaagatgccg
     2281 ggaaatac
//
LOCUS       DAZKGF010000351         2285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZKGF010000351 DAZKGF010000000
VERSION     DAZKGF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.352"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..671
                     /locus_tag="ACHOQV_005123"
     CDS             3..671
                     /locus_tag="ACHOQV_005123"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9633337.1"
                     /translation="MAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNVGRAV
                     TKLPLMGKSLHKTIEKNEVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRGLIV
                     LTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDL
                     EVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEKAITDFMTA
                     RGYH"
     gene            complement(720..2219)
                     /gene="pitB"
                     /locus_tag="ACHOQV_005124"
     CDS             complement(720..2219)
                     /gene="pitB"
                     /locus_tag="ACHOQV_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFP9633338.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          604 a          601 c          569 g          511 t
ORIGIN      
        1 taattgccgt tattggtagc gatggttcag gcaagtcaac ggtgtgtgaa catcttatta
       61 ccgttgtcga aaaatatggt gctgccgaaa gagttcattt aggaaaacag gccggaaatg
      121 tcggtcgtgc agtgacaaaa ttaccgttga tgggaaagtc tctgcacaag actattgaaa
      181 aaaatgaagt gaaaacggca aaaaaattgc cgggtccggt tccggccctg gtgatcaccg
      241 ctttcgtcgc ccgccgactg ttacgctttc gtcatatgct cgcctgtcgt cgtcgcgggc
      301 tgattgtctt aaccgatcgt tatcctcagg accaaattcc tggcgcttac gatggtacgg
      361 tgttcccacc taacgttgaa ggcggccggt ttgtctcatg gctggcaagc caggaacgta
      421 aagcatttca ctggatggcg agccataagc ctgatctggt catcaaactc aatgttgacc
      481 ttgaagttgc ctgcgcgcgt aaacccgacc ataaacggga atcgctggcg aggaagattg
      541 ccataacgcc acagttaacc tttggcggtg cacaactggt tgatatcgac gccaatcagc
      601 cactggaaaa ggtgctggtt gatgcagaaa aagcgattac ggattttatg accgcgcgtg
      661 gttatcacta gtcaaaaatg gaaatgcccg atcgccagga ccgggcattt tcaggaaggt
      721 taaatcaact gcaatgctat ccagtacaac ccaccagaaa gaaaaatcgc cgccggtaaa
      781 gtaaacaccc acgccatcag gatgctggtt accgttttac gctgtaaccc accgccgtcc
      841 accaccatcg tccctgctac cgcagacgag aggacgtgtg ttgtggagac gggcatccca
      901 atataactgg caaggccgat agacactgcc gccgtcattt gtgccgccat gccttgcgca
      961 tacgtcatgc cgcgcttacc aatcttctca ccgatggtca tcgctacacg acgccagcca
     1021 atcatggtgc caatgccgag cgccagagct actgccatga tgatccacac cggagcgtac
     1081 tcaatggtgc ttaacatatc gctgcgcagt tttttcagca ggttctggtc ttctttactg
     1141 acgcctggca gtttcgccag cttcgcggag gtatcagaga tgcacagcat aatgcggcgc
     1201 agctggctgc gctgactcac gcttaacggc tcgtaacttt ccatattgcc tggcagcatc
     1261 gttttaacgc gcgcaatcgc atcaaaggta tttgccggat gacagtgaaa ctccgttact
     1321 tgcgtgccat cagtcgatgc cgcaggcaat ggaggttcca tcgcaatcaa cttctgcggc
     1381 agttcaggat gctgttgcag gtagtgttcg aagttggtga cggcatcgcg ggtacgggta
     1441 atttcatagc cggacgcatt catattgacg acgaaaccag caggggcaat ccccaccagt
     1501 accagcatca ccaggccgat ccctttctgc ccgtcgttag caccgtgcga aaacgccacg
     1561 cccgcagcgg aaacaatcag cgcaatacgc gtccagaatg gtggcttacg tttgcctttt
     1621 ttctttttgc gatcttccgg aatgcggtga atacggtcac gctttttcgt cccgctccag
     1681 aaacggcgca gcaggaaaat cagaccaccc gcaatgacca ggccgacgat aggggaaaca
     1741 atcagcgagg agaaaatttt ggtcacttca cgcaggttta acgcatccat caccgatgag
     1801 ccggttaaca gcgcgttggt taaaccgatg ccgataatcg caccaatcaa ggtgtgcgaa
     1861 ctggaggccg gtaaaccaaa gaaccacgtt cccaggttcc agataatcgc ggccagcagc
     1921 atggaaaaga ccatcgccag gccgtgagtt gaccccatat tcagcaacaa atcggttggt
     1981 aacatatgga caatggcata ggcaacgcta agtccgccta gcaatacgcc aaaaaagtta
     2041 aaaaatgccg ccatcaccac ggcaagctgt ggctgcatgg ctcgagtata aataacggtt
     2101 gccaccgcat tcgccgtgtc atgaaaacca ttgattgctt cgtagaacaa cacaaatgcc
     2161 agagcaagta ataacaaaag ccctgtgtat atatcaaggc caacaaataa atttagcata
     2221 tattagatta cgccattttg aatttacgaa cggacgcatt atcagtgact ttaacggcat
     2281 gggca
//
LOCUS       DAZKGF010000352         2285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZKGF010000352 DAZKGF010000000
VERSION     DAZKGF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.353"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..263
                     /locus_tag="ACHOQV_005125"
     CDS             <1..263
                     /locus_tag="ACHOQV_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFP9633339.1"
                     /translation="RNLSNKIERAEKRLNAGIAGVTAISSIPYVAENSFSYGIGVGNY
                     QNANAVAAGIQYKTSRNTNIRLNISWDSSHNTAIGAGVAGGW"
     gene            843..1106
                     /locus_tag="ACHOQV_005126"
                     /pseudo
     CDS             843..1106
                     /locus_tag="ACHOQV_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1189..1431)
                     /locus_tag="ACHOQV_005127"
     CDS             complement(1189..1431)
                     /locus_tag="ACHOQV_005127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633340.1"
                     /translation="MMMARDITTYHLNSVHISARRFPEIFQWATGMQTKNLPLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
     gene            1671..>2285
                     /locus_tag="ACHOQV_005128"
     CDS             1671..>2285
                     /locus_tag="ACHOQV_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP9633341.1"
                     /translation="MRKISVSYICKSLFLYCSASLPVLSANGISDEMIVELGKHSEAK
                     ANFAVAVGRYSQANAEKSISLGASARAIENNSTALGANATTLKPFSVALGAGSVADRG
                     AGIEAYMPETKRWNPGDVVKSRMSDKQLNRLMEIDKLISAYNSQGTLSAEETKQLEEV
                     KKERADMFATYIATHDAVSVGVGATTTGRHAVTRQITSVAAGSVD"
BASE COUNT          703 a          498 c          478 g          606 t
ORIGIN      
        1 tcagaaacct gagcaataaa attgaacggg cagaaaaaag acttaatgca ggcatcgccg
       61 gagtgacagc aatatcctcc atcccctatg tggctgaaaa ttctttttca tacgggatcg
      121 gcgtgggtaa ctatcagaac gctaatgctg tggccgcagg cattcagtat aaaacatcac
      181 gtaataccaa tatacgcctc aacatctcct gggactcatc tcataacacc gcgattggtg
      241 caggggttgc cggcggctgg taaataatct tgcgtacata cggcattatg gtattccgta
      301 atcacattaa ttcagtacgc aacacagccc catccggaac agaccactgg actgagcggg
      361 ataatcaagg aaaaagggcg cgcagtgtgg tcagccttca ctacaatccg gcggcagatt
      421 tcctgccgcc atgttgcccg taaacattta agttcgcagg ctaacattat tgccacaagc
      481 cccgtatttc cctggtgaat cctcaaagcg agtccctcac catccagaca aaacacagta
      541 tcgtcgatta ataatcacta aaagcgttcg tcgtagcatc ataacccctg aatgaacaca
      601 tccctgtttg caaggattct gttgttctga aatcgacgag tagctgcaac tctgtatccg
      661 cccttattgc agtcagtgca gtctggccac gatgcgattc gctgacctga gccttattct
      721 ccaggcggac tttatgcaat gctcccctga aatcttccat tattttacat gtacaggcat
      781 catattaaat atggaaataa taacgaacga aaaagggaca caatgaatat caaagcacca
      841 gtataaacca ggatgttttg agcaatttct tactctcctg aatacacact gtaaatcatc
      901 agaaactgca ctagccatgc taagtacact ctgctacgaa ctgatgagga agtgcggtat
      961 caattacctg gacagcctga tcaacggaga ggtatatcta ttatctatgc aattacagga
     1021 ggactgatat cagcgaaaaa gcaggtcagc atattcactg cccctggtct gaactgaaac
     1081 aacgtcagcc agaaataatc ctataatgcc tcatataagt ggataacttt tcatccgtcg
     1141 ttgacatcat acacatgaaa cattgataac agtcatgaat acaccgaatt atctttctga
     1201 ctcagcgttt ccagtcagta gcatttcttc cggtaaaggc atttccgaaa cgtcacgttc
     1261 gccattaaat acaaaaatac tttctgcgta atcattctgt aacaaagaat tagttaacgg
     1321 cagatttttt gtttgcatac cggtggccca ctgaaaaatc tcggggaaac gacgggcact
     1381 gatgtgtaca gaattgaggt ggtacgtagt aatatctctt gccatcatca taagatggca
     1441 catcatatcc cgaacgcaaa cctggcacat gatgaacaga cggaaggctc tgcaggactt
     1501 ctggataatt atacacgata gccagttact gctgacctgg atttattatc ataccacatt
     1561 atttatttca aaaaataaat tttaaattat ttgtttttac ttgtatataa atatccaacg
     1621 atggttctgt ttgtattatg tatactatat aaaagagatg gtgaattcaa atgcgtaaaa
     1681 tcagtgtcag ttatatatgc aaatcattat ttctctactg ctcagcttct ttacctgtct
     1741 tatctgcaaa tggtatttct gatgaaatga tcgtggaact tggtaaacac agtgaggcaa
     1801 aggctaattt cgctgttgct gttggtcgat actcacaggc aaatgcagaa aaaagtattt
     1861 ctttaggtgc atcagcaaga gctattgaaa ataactctac cgcgttagga gcaaacgcaa
     1921 caacattgaa accattctct gtcgctctgg gcgcaggaag tgttgctgat cgtggcgctg
     1981 gtattgaagc atatatgcca gagacaaagc gctggaatcc aggggatgta gtaaaatcca
     2041 ggatgtctga caagcagctt aacaggctta tggaaatcga taagctaata tccgcttata
     2101 attcacaagg gacgctgtct gctgaagaga ctaaacaact ggaggaagtg aaaaaagagc
     2161 gggctgatat gtttgcgaca tatatagcaa cgcacgatgc tgtgtctgta ggtgttggtg
     2221 caacgacaac aggacgacat gctgttacaa gacagataac tagcgttgca gcaggttctg
     2281 tcgat
//
LOCUS       DAZKGF010000353         2269 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZKGF010000353 DAZKGF010000000
VERSION     DAZKGF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.354"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..705
                     /locus_tag="ACHOQV_005129"
     CDS             <1..705
                     /locus_tag="ACHOQV_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFP9633342.1"
                     /translation="HPRQYGPRCTLTVDTDAVNVAQLRDLQTVTEKANAALDKRITNN
                     TSAITKNLQVVNSNKEEISNINKTTKILDERVTANTQTAEEHKLLISENSKQISTNAL
                     GIRENTQRIRDEGQARIRGDRETLRAATGYTDMRVNGLERNTNKRFAQLKEDIDKNRK
                     RADAGIAGAMAMTGIPSVPGKKVSFGMAMAGYRSEGAVAAGFHFNTSENSAVKVNAAW
                     DSESGVGVSAGMALGW"
     gene            complement(1134..1709)
                     /gene="dolP"
                     /locus_tag="ACHOQV_005130"
     CDS             complement(1134..1709)
                     /gene="dolP"
                     /locus_tag="ACHOQV_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP9633343.1"
                     /translation="MRLLYSAVVLTSALLLQGCVAAAVVGTAAVGTKAATDPRSVGAQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADIASRVSGVKRVTTAFSCIR"
     gene            complement(2031..2258)
                     /locus_tag="ACHOQV_005131"
     CDS             complement(2031..2258)
                     /locus_tag="ACHOQV_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFP9633344.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
BASE COUNT          622 a          549 c          517 g          581 t
ORIGIN      
        1 catccccgtc aatacggccc tcgctgtacg cttactgtcg atacggatgc tgttaacgtt
       61 gcacagctca gggatttgca gactgtgaca gaaaaggcga atgcagcgct tgataaacgc
      121 ataacaaaca acaccagtgc gataactaaa aatctgcagg tggttaacag taataaagag
      181 gaaatcagta acattaataa aacgacaaaa attctggatg aaagagtgac agccaataca
      241 cagacagcag aagaacataa gctgcttatt tcggagaaca gcaaacaaat cagtaccaac
      301 gctcttggta tcagagagaa tacacaacgg atccgggatg agggacaggc tcgaatccgg
      361 ggagacaggg aaacactgcg tgcagcgacc ggttataccg acatgcgggt aaacgggctg
      421 gagcgtaata cgaataaacg ttttgcgcag cttaaagagg atatagacaa aaacagaaaa
      481 cgtgcagatg caggtattgc aggtgcgatg gcaatgacgg ggataccgtc cgtccccggg
      541 aaaaaggtct catttggtat ggccatggcc ggataccgga gtgaaggtgc tgttgctgcc
      601 ggcttccatt ttaatacaag tgaaaacagt gctgttaaag tgaatgcagc ctgggacagt
      661 gaaagtggag tgggcgtttc agccgggatg gctctgggat ggtaatgctt ccgggccaga
      721 agccgttcgc agatattgtt atccagggga cactgcacca ccattcagga agcgattcag
      781 tttatcgcat cggctcagga tgtaattaat acggacaatc tggctcctgc accggagcca
      841 gacgtacacg tgtcaacgcc acgatctttg acctgtatgg ataaaagctc agattattta
      901 ccagcagcga catccggagc gcaccgggat tttatctgtg agtgaacata cccgggcaaa
      961 aaaatccccc gaattcaggg gctgtcaaag aggaattatg tatattggtt attataatga
     1021 gcacgcctcc ggtattccgc gacgaatgtt cacgtgctgg catgaacggc ccggaaacgt
     1081 gctcttctga tttttaacgt aaatttacac taaaggcaac tctgatacgg ccttcaccgt
     1141 atacaggaga atgccgtcgt gacccgcttc acgccactca ccctactggc aatatctgct
     1201 gccgctcgcc cttcctgctc tgtgaccagg cccagtaaaa agacttcgct gttttctgtc
     1261 accaccttga cactggatga tttgacacgg gcacttgtga gtaactgaga acgaattttt
     1321 gtcgtgagcc aggcatcacc agccattatg accggactga cgggagtccc ctgccggatt
     1381 tcattataga cctcctgaac accatcgaca ccgagtgcta tctgacgggc acgggcagat
     1441 aattcacggt ccggagactg accgatcagc agcacgcgtc cctgatatgc aatctcactg
     1501 atgcgtgctt cttttctgat ttgttcatct tttgaaagtg cactgctgac cctcagctcc
     1561 agcacttcat catccacctg cgctccgaca ctgcgtgggt cggttgctgc ctttgtcccg
     1621 acagcagctg tacccaccac agcggcagcc acgcacccct gtaacaataa ggctgaagta
     1681 aggacaacag cagaatataa taaacgcata tctcccccgg aattgttttg atttttactt
     1741 catattcccg gaatttatcg tacaaaaagt ttaccttcag tgaaacaaaa caagctacag
     1801 actgttatta tcctccggca cagccggaaa tagcggatgc gttacgtggc atatctgtat
     1861 ctgttttttg cacgaaaact ttgataccgt cgtaaagatt ctttttttaa ctatctggtt
     1921 cgctgaacag gatctccttt tcgttataca caggctaaaa ggcaccgtgg agcccccggt
     1981 gcctttttca taaaacagag aaaacacgtc tttgaatata ccatttatga ttaccgttcg
     2041 cccttttctg actcatcttc aaggattttc tggatttctc ttttgatctc aagtttttca
     2101 agcttcagcc tgaccacttt cgtcgaatac gctgaaccaa attcatcttc aattcttttt
     2161 atttcgtgat caagtttatt atgcttttca aataaagaat caaagcgttt atgatttcct
     2221 ttcaatgctg aaatcagttc tctgtactcc gggaacattg tttttatct
//
LOCUS       DAZKGF010000354         2186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZKGF010000354 DAZKGF010000000
VERSION     DAZKGF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.355"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            94..273
                     /locus_tag="ACHOQV_005132"
     CDS             94..273
                     /locus_tag="ACHOQV_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP9633345.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            359..859
                     /locus_tag="ACHOQV_005133"
     CDS             359..859
                     /locus_tag="ACHOQV_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9633346.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETYGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            905..1411
                     /locus_tag="ACHOQV_005134"
     CDS             905..1411
                     /locus_tag="ACHOQV_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9633347.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNDEKHMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1470..2108)
                     /gene="ompW"
                     /locus_tag="ACHOQV_005135"
     CDS             complement(1470..2108)
                     /gene="ompW"
                     /locus_tag="ACHOQV_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFP9633348.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT          694 a          485 c          473 g          534 t
ORIGIN      
        1 atttgactaa ttttcattag cgactaattt taatgagtgt cgacacacaa cacccatatt
       61 aatgaaacaa tgcaacgcaa cgggagaaat aacatggccg aacatcgtgg tggttcagga
      121 aatttcgccg aagaccgtga aaaggcatcc gacgctggcc gtaaaggcgg tcagcatagc
      181 ggcggtaatt ttaaaaatga tccgcaacgc gcatctgaag cgggtaaaaa aggcggtcaa
      241 caaagcggtg gtaataaatc aggcaaatcc tgattcaccg ttaattatta atatcagctg
      301 ataaagataa ttgcatctgc gggccgtatg gctcgcagtg atttcactgg agaaaaatat
      361 gaatatgaag accattgaag atgtatttat tcacctgctt tcagatacct acagcgcaga
      421 aaaacaatta acccgggcac tggcaaaact cgcaagagca acatcaaacg aaaaattaag
      481 tcaggctttt catgcgcacc tcgaggaaac atatggacag attgaacgta ttgatcaagt
      541 tgtggaatca gaatcgaatc tgaaaattaa gcgcatgaaa tgtgtggcaa tggaaggtct
      601 tattgaagaa gctaatgagg tcatcgaaag taccgagaaa aacgaagtgc gtgatgccgc
      661 actgattgcc gcagcacaga aagtcgagca ttatgagatt gccagttacg ggacattagc
      721 gacgctggct gaacaattag gttatcgtaa agcagcgaag cttctgaaag agaccctgga
      781 agaagaaaag gccaccgaca tcaaattgac tgatctggcc cttaataacg taaataagaa
      841 agccgaaaat aaagcctgaa atatgaactt taacttttag tcattttata aagaggacat
      901 tttcatgaat cgtattgaac attatcatga ctggttacgt gacgcccacg caatggaaaa
      961 gcaagccgaa tcaatgcttg aatccatggc cagtcgtata gataattatc ctgaactacg
     1021 cgctcgtatt gaacaacatc ttagtgaaac caaaaaccag attgttcaac tggaaactat
     1081 tcttgatcgt aatgacattt cacgttcagt cattaaagat tccatgagta aaatggctgc
     1141 acttgggcag tcaatcggtg gtatattccc ttctgatgaa atagtcaaag gctctattag
     1201 cggatatgtc ttcgagcaat ttgaaatcgc ctgttatacc tcactcttag cagcagcaaa
     1261 aaatgccggt gatacagcct caattccgat tatcgaagcg attttaaatg atgaaaagca
     1321 catggccgac tggctaattc agcatattcc gcaaacaact gagaaattct taattcgctc
     1381 tgaaactgat ggcgtagaag cgaagaaata atacccttta taccatgtcc ttattgaccc
     1441 cgtaattacg gggtcatttt tgtgcggaat taaaaacgat atcctgctga gaacataaac
     1501 acccacggat cgaggcgtac gctatcgtgt tgctgtgcac cgcccagttt ataattggca
     1561 gtggtatcaa tatccatgta ccagactgac atgttaacca accagtcacg gttaatcaga
     1621 taatcaaccc ccacctgccc ggcagctccc caggaatctt tcagactgag atcggaaagc
     1681 cccgcctctt tgccatgatc gttaaatcca ttatcaaaga aggtggtgta gttaatacct
     1741 gccccaacgt aaggacggaa tttgctgctg gcatcaccaa aataccactg cgccatcagt
     1801 gttggcggca gatgatgaac ggttgcaata tcgccggtcg cccgggtgcc gattttatgg
     1861 cggaacggcg tcgctgccag caattccaca ccaatgttat cggtcgccat ataagtaaac
     1921 gtcaggccca gttgcgtatt attggtcacg ctgaatccac ccagacttcc taacgtacca
     1981 ccagcacctt ctgttggacg tacggttgca gaacctgcac gcataaaaaa ttcgcctgct
     2041 tcatgcgcaa aggcactgcc agagagaaga gttgttactg ccaaagccgc cactgttaac
     2101 tttttcatat ccgctccgtc gttatggtta taaaaacgtg acgaatatac ctacattgga
     2161 gtaacaaatg atcctacata gatcac
//
LOCUS       DAZKGF010000355         2151 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZKGF010000355 DAZKGF010000000
VERSION     DAZKGF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.356"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..588
                     /locus_tag="ACHOQV_005136"
     CDS             <1..588
                     /locus_tag="ACHOQV_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm72 family immunity protein"
                     /protein_id="HFP9633349.1"
                     /translation="PENLPPVPERSDIIIKTDDPVPCDGIWEPVKIHYDHKLLIIKDG
                     ISGFNNLGAYNYFIRGMNAPRQVYYDVLIESDTEMVVPDDPIRYRDVHWRLVWEDTRY
                     SDGIIPDESEYFLDDAPGKRITCPSGELCPHSGHWATIAGGHQQFIDVQAGQLMPEAT
                     KYQSNMYADEIHLPAIWNLLRRDDEGSVYVKTGES"
     gene            666..1088
                     /locus_tag="ACHOQV_005137"
     CDS             666..1088
                     /locus_tag="ACHOQV_005137"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFP9633350.1"
                     /translation="MDGQRYFGESLSVQQPAAVPLFDTWPDSRIAFAGPWVMEMNSIT
                     DFRERLCELEAALPSVSWMISSSSLTELAAHFRRNMNTELPDGRIALLRFHDPRVQKR
                     LGEMLNDQQHRELTGLMQEWLTIVDGKAWSFKQREFIC"
     gene            1082..1426
                     /locus_tag="ACHOQV_005138"
     CDS             1082..1426
                     /locus_tag="ACHOQV_005138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633351.1"
                     /translation="MLKLSEEQCVGMDKREDDDFVRDTALRLKKKFPGITESDDTFHV
                     RLRKALDYANTLPLKEKNVKRDFLFLEAFWPGFYLKPEVDKWLRTPNAYSTDQRVEDY
                     KHVMINRERREL"
     gene            1426..>2151
                     /locus_tag="ACHOQV_005139"
     CDS             1426..>2151
                     /locus_tag="ACHOQV_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tox-REase-5 domain-containing protein"
                     /protein_id="HFP9633352.1"
                     /translation="MVMVLTGAGMGSAATGGVGMAGASTGTAVFPGAGSRDGDWEEDN
                     ISYPGSGSGSRQGNGGFYYQGKQYDPILNEPMVVSAETHGDFWEDDMDHSETVNYEGI
                     DSKAEVQAKTDEKTCENCPPEGKVMPMIRRCSRWSMVTISYQTRICGTFYNPETQQIQ
                     EFKYCGVSFDGWKDKLCQFWEAKARYDQFFRADGSKKPWWTGNLSAINQASRHQAVAT
                     VNQPLKVVWIFMQPVSCEYFRKMF"
BASE COUNT          600 a          421 c          589 g          541 t
ORIGIN      
        1 ccagaaaatc tcccccctgt tccagaacgc agcgatatta ttattaaaac cgatgacccg
       61 gttccctgtg acggcatctg ggaaccggta aagattcatt acgaccataa actattgatt
      121 attaaagatg gtatcagcgg atttaataac ctcggtgctt ataattattt tattcgtgga
      181 atgaatgctc ctcgccaggt gtattacgac gtattaattg aaagtgacac agaaatggtc
      241 gtacccgatg acccaatccg ttaccgggac gttcactggc gtctggtctg ggaagacacg
      301 cgctacagtg acggaattat tcccgatgag tcagaatact ttctggatga tgcaccaggc
      361 aaacgcatta cctgcccgag tggggagctt tgcccgcatt caggtcattg ggcgaccata
      421 gccggaggac atcagcagtt tattgatgtt caggccgggc agttgatgcc agaagcaact
      481 aaatatcaga gcaatatgta tgcagatgaa atccatcttc ctgcgatatg gaacctttta
      541 cgccgggatg atgaggggag tgtttatgta aaaacagggg aaagttaaat aactgatcaa
      601 cgctttttga cggacagatc tgttaccacc tgatttgctc aacaaaagtg acattatttg
      661 ctctggtgga tggtcagcgg tatttcggtg agagcctgtc tgttcaacaa cctgccgccg
      721 tgcccctgtt cgatacctgg cctgattcgc gtattgcatt tgcggggcca tgggttatgg
      781 agatgaacag tataacggat ttcagagaaa gactgtgtga gcttgaagct gcgcttccgt
      841 cagtttcctg gatgatatct tcttcctcgc ttactgaact agctgctcat ttcagaagga
      901 atatgaatac agaactaccg gatggacgga ttgcgttgct gcgtttccat gatccgcgcg
      961 ttcagaagcg cctgggagaa atgctgaacg accaacaaca tcgggagctg acggggttaa
     1021 tgcaggagtg gctgacaata gtggacggaa aagcctggtc atttaaacaa agggagttca
     1081 tatgctgaag ttgtctgaag agcagtgtgt tggtatggac aaaagggaag atgatgattt
     1141 tgttcgcgac accgcgctaa gactgaagaa gaaatttccg ggaattactg aatcagatga
     1201 cacgttccat gttcgcctga gaaaagcgct ggattatgcc aatacccttc cgcttaaaga
     1261 gaaaaacgtg aagcgggact tcttgtttct ggaggccttc tggcctggtt tttatctgaa
     1321 acccgaagtg gataaatggt tgagaacgcc gaatgcttat tcaactgacc agcgggttga
     1381 agattataag catgtgatga taaacagaga aaggagggag ctctgatggt tatggtcctg
     1441 acaggggctg gtatggggag cgcagctacc ggaggtgttg gtatggctgg tgcctcaacc
     1501 ggaacagcag tatttcccgg agcaggaagc cgcgatggag actgggaaga agataatata
     1561 tcttatcctg gctcaggcag tggttcgcgt cagggtaacg gagggtttta ttatcagggg
     1621 aaacagtatg atccaattct gaatgagccg atggttgtca gcgcagagac gcatggtgat
     1681 ttctgggaag atgatatgga tcattcagaa acagtaaact atgaaggtat tgatagcaaa
     1741 gccgaagtac aggcgaagac ggatgagaaa acgtgtgaga actgcccacc ggaagggaag
     1801 gtaatgccga tgataaggcg ttgctcgcgc tggagtatgg taacaatcag ctaccagaca
     1861 agaatatgtg gcacgtttta taatccggag acgcaacaga tacaggaatt taagtactgt
     1921 ggcgtatcgt ttgatggctg gaaagataag ctgtgtcagt tctgggaagc taaagcgcgg
     1981 tatgaccaat ttttcagggc tgatggttca aagaaaccct ggtggacagg taatctaagc
     2041 gcaattaatc aagcatcccg acatcaggcg gttgcaacag tcaatcagcc attgaaagtt
     2101 gtctggatat ttatgcaacc tgtaagttgt gagtatttca ggaaaatgtt t
//
LOCUS       DAZKGF010000356         2083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZKGF010000356 DAZKGF010000000
VERSION     DAZKGF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.357"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..500
                     /locus_tag="ACHOQV_005140"
     CDS             <1..500
                     /locus_tag="ACHOQV_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633353.1"
                     /translation="NCSICKKMVPFYSDDNCLLCNKKNEELSPTRDEINKIREFDVTI
                     SQDNLNSVTQRLLPTPLAAVRARRVAFENPYRNKVLSESLPAGNLLYRSALAFIQALI
                     ALVPSKKILDLDKITDALRRRYVHLEQLEYAELRNALACALSTCYNRKYLIKVAKGKY
                     LPVDD"
     gene            582..929
                     /locus_tag="ACHOQV_005141"
     CDS             582..929
                     /locus_tag="ACHOQV_005141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9633354.1"
                     /translation="MPGQPIPSELKEKGLIWLLPPYCWSHRQIARKLKISASVVSKWR
                     YELVEQGLLPENEKITSVDVEDWTPERRFSVVLETATLSEMELADYYRRMGLFVEQVK
                     EWRAISIQAHEKN"
     gene            1102..1266
                     /locus_tag="ACHOQV_005142"
     CDS             1102..1266
                     /locus_tag="ACHOQV_005142"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9633355.1"
                     /translation="MERRKIIALVREAVSNGARKSQACRCIGISIRTLQRWTDGNVIT
                     TDVSVNGAPY"
     gene            complement(1270..1737)
                     /locus_tag="ACHOQV_005143"
                     /pseudo
     CDS             complement(1270..1737)
                     /locus_tag="ACHOQV_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1801..>2083
                     /locus_tag="ACHOQV_005144"
     CDS             1801..>2083
                     /locus_tag="ACHOQV_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9633356.1"
                     /translation="MEQKTLSAEPRRSFSNNFKLQMVKLALQPRVSVARIARKHDIND
                     NLLFKWLRLWLIKGRISRRLPVRNSSGIGVELLPVEMTLDERPYPAFYAR"
BASE COUNT          595 a          458 c          481 g          549 t
ORIGIN      
        1 aaaattgtag catatgtaaa aagatggtac ctttttattc tgatgataat tgtttgcttt
       61 gtaataagaa gaatgaagag ttgtcaccaa ccagagatga aattaataaa attcgcgagt
      121 ttgatgtaac aatatcccag gacaatctta attcagttac tcaacgattg ttacccacac
      181 cattagctgc ggtaagagcc cgaagagttg catttgaaaa tccatataga aataaagtac
      241 taagtgaatc attacctgct ggaaaccttc tttacagaag tgcattagct tttatacagg
      301 ccttgattgc attagtccca tcaaagaaaa tactggactt ggataagata acagatgctc
      361 tgcgtaggcg gtatgttcat cttgagcaat tagaatatgc cgaattaaga aatgctttag
      421 cttgtgcatt gtcaacttgt tataatagaa aatatctcat taaagttgca aaaggaaaat
      481 atttacctgt tgacgactag gtgcgtatta ccgtgaagga gatcggtgag taaccccctg
      541 tgtcagcata gttgtcgcct caatttttta aggagataac aatgccagga caaccaatac
      601 caagtgagct caaagagaaa ggactgatat ggcttttacc accatactgc tggtcacatc
      661 gtcagatagc ccgtaagcta aagatcagtg cctcagttgt atcaaaatgg agatatgagt
      721 tagtggagca agggctttta cctgaaaatg aaaagattac ctccgttgat gttgaagact
      781 ggacaccgga gcgtcgattc tcagttgttc ttgagacggc tacgctgtca gaaatggagc
      841 ttgctgatta ttaccgacgc atgggattgt ttgttgaaca ggttaaagaa tggcgggcta
      901 tctcaattca ggcgcatgaa aaaaattgac ggaaaatatt cgaacagaca gggcattgcg
      961 tgacgcaaaa aataagttac gagaactgta gaaagagctt aagcgtaaag ataaagctct
     1021 ggcggaagct gcggccctgc ttatattgag ggaaaagttc aatgccctct gggacaacag
     1081 cgaggaagac tgacccctct cctggagcgg cgtaaaatta ttgcccttgt ccgcgaggcc
     1141 gtctccaacg gcgcccggaa aagtcaggct tgtaggtgta tcggtatttc aattcggaca
     1201 cttcagcgct ggactgatgg caacgttatt accacggatg taagcgtcaa cggagcaccg
     1261 tattgacgct tatttattgg tgagtactac gttccatggc aggagttcat caactcggtt
     1321 ggaaggccat tccggcagta cgctcaggat aaggcgcaga tacgcttccg gatcgatacc
     1381 gttcagacgg caggtgccga tcagcccgta caacagtgct ccacgctcgc cgccgtgatc
     1441 gctgccaaag aacataaagt ttttctttcc gagacagact gcacgaagcg ctctttccgc
     1501 agcattatta tccgcctccg ccagaccgtc atcactgtaa taacagaggg catcccactg
     1561 attcagtaca tagctgaacg cttcgcccag tctggatttt ttcgacagcg taccattctt
     1621 ctccaccatc cattcatgca gcgacgtcag taacactttg cttcgctgct gcctgacgga
     1681 aagacgctct gactccggta atccccgtat ttcgtaagcg taaaccgacc gccgtatgta
     1741 gccattaagc cagtattggt aacgtaggtg cccacctttt caaactagtg gacgcctgtt
     1801 atggagcaaa aaacattatc cgcagaaccc cgcagatcat tttcaaataa ttttaaactt
     1861 caaatggtta aactggcttt acaaccaagg gtttctgttg cccgtattgc ccggaagcat
     1921 gatatcaatg ataatcttct gttcaaatgg ctcaggctct ggctgatcaa agggcgtata
     1981 tcacggcgtc ttccggtaag gaactcttct ggcattggcg ttgaattatt acctgtggag
     2041 atgacgctgg atgagcggcc ttaccccgct ttttacgcga ggt
//
LOCUS       DAZKGF010000357         2079 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZKGF010000357 DAZKGF010000000
VERSION     DAZKGF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.358"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..785
                     /gene="nlpA"
                     /locus_tag="ACHOQV_005145"
     CDS             <1..785
                     /gene="nlpA"
                     /locus_tag="ACHOQV_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFP9633357.1"
                     /translation="ALLLAGILLAGCDQSSSDEKHIKVGVINGAEQDVAEVAKKVAKE
                     KYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGYKLVAVGNTFVFP
                     MAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKEGKGLLPTALDIT
                     DNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDSVFIEDKNSPYVN
                     ILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(789..1712)
                     /locus_tag="ACHOQV_005146"
     CDS             complement(789..1712)
                     /locus_tag="ACHOQV_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFP9633358.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(1879..>2079)
                     /locus_tag="ACHOQV_005147"
     CDS             complement(1879..>2079)
                     /locus_tag="ACHOQV_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633359.1"
                     /translation="LKNEKTYVSLPNPTILITTTSTPERQFSISVLDDDNKENLPTDT
                     APCFTIGIPDEYTKAIELLNIK"
BASE COUNT          640 a          445 c          500 g          494 t
ORIGIN      
        1 ccgcattatt actggcagga attctgctgg caggttgcga ccagagcagc agcgatgaaa
       61 agcacattaa agtgggcgtt attaatggcg cagaacaaga tgtcgcggaa gtcgctaaaa
      121 aggtggcaaa agagaaatat ggcctcgatg ttgaactggt aggatttagc ggttcgttgc
      181 taccgaacga tgcgacaaat catggcgagc ttgatgcgaa cgtcttccag catcgaccct
      241 ttcttgaaca agataatcag gcgcatggtt ataaactggt agcggtggga aatacgtttg
      301 tcttccccat ggcgggttat tccaaaaaaa tcaaaaccgt ggcgcaaata aaagaaggcg
      361 cgacagtggc gatccccaac gaccctacca accttggtcg cgcgctttta ctgttgcaaa
      421 aagagaagct aattactctg aaagagggaa aagggttact acctaccgcg ctggatataa
      481 ccgataatcc acgccatttg cagataatgg agctggaagg ggcacagttg ccacgtgtac
      541 tggacgaccc gaaagtggat gtagcgatta tcagcaccac ttacatccag caaacaggac
      601 tttctccggt taacgacagc gtatttattg aagataaaaa ttcgccgtat gtgaatattc
      661 tggtggcacg ggaagataat aagaatgcag aaaacgtgaa ggaatttctg caatcttatc
      721 aatcacccga agtcgctaaa gcggcagaaa ccatctttaa cggaggcgcg gtgcctggct
      781 ggtaattctc actcatgccg cgccggacga tttatttttc tggcgcggcg gcgggaatcc
      841 attgaaatca aaatcaccga tgacagaatc agcagcgttc ccagccagtc cggcaatgtg
      901 aaggtgatcc ccagcaacag caaagagagc agcgcgctac ttaatggttc tgcacagctt
      961 aaaatgctgg cttttggacc gccaattaat tgtgctcctt tcaggtacag actaaatgtc
     1021 agggacgtac caatgaccac caaataaaaa aacgccagaa tcaaactgcc gttaacgaca
     1081 aagtttgtcc cttgtctggc ataaaagggc agcaggatca gaccgccaat cagcatactc
     1141 cagccgacga ccggtaacgt gccatagcgg gcaatcagcg ttgagggata ggtggtatag
     1201 aatgcagcag caaatgccga ggcaatgccc cagaataacg cggcaggaga gatcgataat
     1261 gacgtcggat taccgtgtgt caccaataaa aaagtcccga tcagcgatgt caaaatagcg
     1321 cagaaaacca gaatgcccgg gcgggattta cgcaccagtg agaaccaggc gacgataata
     1381 gtcggtgaga ggaattgcag caccgttgcc gtggctgcgt tcgatttttc tatggttagc
     1441 aaaaaagtga gctgtacggt tagcgcgcca accacggaaa aaatcagtag gctaatggca
     1501 tctttgtgat tgttaataat agaaaagatt ttatcaccat gaacaaatga tagcgtcagc
     1561 agaatcaaac cggcgaatat caaacgcgtc atagtcaaaa actgcgacga catctggctt
     1621 tgctccatga tgtattgcgc gcagacccct gaacttcccc acaacacggc ggcaatcaga
     1681 acgttcagca tcccctttct ggtggaaccc atttttccct ctgcatactg ttttgtcttt
     1741 tcatcaatat acgttaagga taccactacc gatgacggtg agggagtaga gtaatcaatc
     1801 agttttagtg aatggaggaa ataatgacat ataacaatta ttcatatggt atttaaaaaa
     1861 actcaatatg aaatttaatt atttaatgtt caataactct atggctttag tatattcatc
     1921 aggaatacca attgtaaaac aaggtgcggt atcggtaggt agattttctt tgttatcatc
     1981 atccagaaca gaaatagaaa actgtcgttc aggtgttgat gtcgtggtta ttaaaatggt
     2041 tggattagga agagaaacat atgttttctc attttttag
//
LOCUS       DAZKGF010000358         1986 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZKGF010000358 DAZKGF010000000
VERSION     DAZKGF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.359"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(211..378)
                     /locus_tag="ACHOQV_005148"
                     /pseudo
     CDS             complement(211..378)
                     /locus_tag="ACHOQV_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            528..1196
                     /locus_tag="ACHOQV_005149"
     CDS             528..1196
                     /locus_tag="ACHOQV_005149"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9633360.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGIVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1562..1675
                     /locus_tag="ACHOQV_005150"
     CDS             1562..1675
                     /locus_tag="ACHOQV_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFP9633361.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            1744..1977
                     /gene="ydfK"
                     /locus_tag="ACHOQV_005151"
     CDS             1744..1977
                     /gene="ydfK"
                     /locus_tag="ACHOQV_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFP9633362.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNKNLNGKM"
BASE COUNT          668 a          414 c          335 g          569 t
ORIGIN      
        1 attcaacaac agtaaaaaaa cattattaga accattcaat taacaaaaaa accaacatcc
       61 agcttgctta atttttcttt attaaacgat attgaaaatc aattgataaa atacatctaa
      121 acaacctttt ggggcgcaaa agcataacat caaacaaata acacaccgaa aaaactcaca
      181 attaataacc tatgatatac atactgttta ttatggttga ataagccact cgatatctgg
      241 tgctacggaa gtgtccacac ggtttagcag cacccgatac ttttttcagg cttccagcaa
      301 cgatctttct tcctccgttg cgatttccag atctacagca tcctgcagtg gcgcaatata
      361 ctcactgaat tcctggatgt agaactgtgt ggtgacggtc ttccagctat tcggctcctg
      421 ctgtattgaa gcataccagg ctatttcaat atcgctatgc tgcggcagca tttaacccct
      481 tgtaattcat tgccataatt gatttaattc acaaataaaa ctataacatg gtgaaattaa
      541 tgaaaaaaaa cacagatgat ggggctaaaa tttacacacc acttacccta aagctttatg
      601 actggtgggt tttgggagta tcaaatcggc ttgcatgggg atgtcctaca aaggaacacc
      661 ttcttccaca ctttctggaa catttaggta acaaccatct ggatattggt gttggaactg
      721 ggttttacct tactcacgta cctgagagta gtctgatatc tttaatggat ttgaacgaag
      781 ctagcctgaa cgcggcatct acaagggctg gggaatcaaa aattaaacat aaaattaacc
      841 atgatgtttt tgaaccttat cccgcggcgt tacatggtca atttgattcc atttccatgt
      901 tttaccttct tcactgcctg cctggaaata tatctacaaa aagctgtgta atacgcaatg
      961 cggcgcaggc cttaactgac gatggaactc tatacggagc cacaattctt ggcgatggaa
     1021 ttgtgcacaa tagcttcggt caaaaactga tgcgcattta caatcagaaa ggcatctttt
     1081 caaacacaaa agattccgaa gaaggcttaa cacatatact ctcagagcat ttcgagaatg
     1141 ttaaaaccaa ggttcaaggt actgtagtaa tgttttccgc ttcagggaaa aaatagcatc
     1201 caaccgcagc acgttcttgc ataaggcgtg tctgcggttt ttcaactatt cagatacatc
     1261 actcccatca cattcattcc tccgcatcat aggcatatag gctatatcac cttgatattt
     1321 ttcttcttca gataaaaact gttatctatg tatactttta aacccaatcc gtgtagagtc
     1381 tctgcataag atagtttgca gttgacactt cagcttgcgc cataaaccgc ctgatttttg
     1441 ccgccacctg ttagcattcc tgtatacctg aaacgacaat gtttatctac gaactttaag
     1501 aacacccagg ataaaaattg tcaactatat catatataac acattactaa ttcgaggcta
     1561 tatgaacagc atactgataa tcacttcgct ccttatcata ttcagcattt ttagtcatgc
     1621 tctaataaaa ttagggattg gcatatccaa taacccagac aaaaccgatg tataagtcaa
     1681 catatcctga atcagacata caatatcgca atgaaaatca ataatatttt aaggaatatc
     1741 ttcatgaaat caaaagacac cctaaagtgg ttccctgcgc agcttcctga agtaagaatt
     1801 atcctagggg atgctgtagt ggaagtagca aaacagggaa gacctatcaa taccagaaca
     1861 ttgcttgatt acattgaagg aaacataaag aaaaagtcat ggctggataa caaagaatta
     1921 ttacaaacag cgatatcagt tcttaaagac aacaaaaatt taaatggtaa aatgtaatat
     1981 aataaa
//
LOCUS       DAZKGF010000359         1909 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZKGF010000359 DAZKGF010000000
VERSION     DAZKGF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.360"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(501..1592)
                     /locus_tag="ACHOQV_005152"
     CDS             complement(501..1592)
                     /locus_tag="ACHOQV_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9633363.1"
                     /translation="MITCVFIFTAILLAVITFIPVSGNTSWVVRVWDFPRLQILLLCT
                     LNVFLSVVFLDSFAELFVVIISLICVAYQLVWIIPYTVIGKKQVKEIKDSDGFPGLSI
                     LVFNVLMTNKKYMLLLELINKEKPDILVLLETNLWWEQKLSPLDEVFPYSLKCPKENL
                     YGMHVYSRMILSDAEIQYLVDKEIPSMHMIVHMPEGQRIRLHCLHPVPPSPTESDEST
                     DRDSELLMVGKSVADSELPVILAGDLNDVAWSATTRLFMKISGLLDPRRGRGFFSTFH
                     SHYFFLRWPLDHVFHSQHFLLKSLRRLPDMGSDHFPVMAELVYAPAHGMQQESLEKEK
                     SDEVLAQKKMDMTDSTAEDVHTPGGQKKS"
BASE COUNT          585 a          448 c          339 g          537 t
ORIGIN      
        1 gtgccgggtg cctcccggtg aacagactgt tcgtgatacc agtcagcaac ataggaaatt
       61 aatggtatca ccccaccgca cagggggatt caccatgcct gcctttttta gcaaattcat
      121 cagaaaccgg acaacatgct tatttatgaa atgaggtgct tttcacattt atataaaatc
      181 attataacga taatcctaaa aatcaatgta attatcatct tcagtaactt tctgtattac
      241 accatacgga aattgatttt tgacggtaaa gatacaaggg gaaatatgtc tgtaaggagt
      301 accctgaata atggacaggc atgcatcgtg gctgttcccc tgaaattatc agtacaacca
      361 gtcagctcat aatccctgtc tctttttccg gggagccctc agggggactt tcccttatcc
      421 ccccagtttc tggagagacg taaaaaagaa ttaatcatat gtcaggtatc tgaatatgtc
      481 ccgtaattaa tatatttacg tcaggatttt ttctgcccgc caggagtatg aacatcttcc
      541 gccgttgagt ctgtcatatc catttttttc tgtgccagta cttcatctga tttttctttt
      601 tccagactct cctgctgcat cccgtgagcc ggagcatata caagctcagc catcactgga
      661 aaatgatctg aacccatatc cggtaaacgg cgtaaacttt taagcagaaa atgctggctg
      721 tgaaatacat ggtcaagcgg ccatcgcagg aaaaaataat ggctgtgaaa cgtgctaaaa
      781 aaaccacgtc ccctgcgtgg atccagtagt ccacttattt tcatgaataa acgagtggtc
      841 gcagaccacg caacatcatt cagatccccc gctaatatta caggtaattc agaatcagca
      901 acacttttcc cgaccattaa aagttctgag tcacgatcag ttgactcatc actttcagtc
      961 ggactcggtg gaacaggatg gagacaatgt aatcttattc tctgaccttc cggcatatgt
     1021 actatcatat gcatggaggg aatttccttg tccactaaat actgtatttc tgcatcactt
     1081 aaaatcatcc ttgaatatac atgcatgcca tataaatttt ctttcgggca tttcagggag
     1141 tatggaaaca cttcatccag aggtgataat ttctgttccc accataagtt tgtttcaaga
     1201 agaacaagta tatcaggttt ttctttattt ataagttcaa gcaaaagcat atatttttta
     1261 ttggtcataa ggacattgaa taccagtata cttaaccccg gaaatccatc agagtccttt
     1321 atttccttta cctgcttctt gccaattact gtataaggaa ttatccatac caactggtaa
     1381 gcgacacaaa ttaaacttat tatcacaaca aacaactctg caaatgagtc aagaaaaaca
     1441 acagacagaa aaacattcaa agtacacagc aaaagtatct gtagtcgggg aaaatcccat
     1501 accctgacaa cccatgatgt attaccggaa acagggataa aagttatgac tgccagaagg
     1561 atagcagtaa aaataaaaac acaagttatc acaaatcact ccttgttctg aaccggcaca
     1621 caaaactgtc atatacgttt caaaagtaaa aacacactgc tgccacaaga ttcacgacgt
     1681 aaccgggcag catatcctga ttacggacaa tccatgaaac cgcctcgcca gaagcgtcca
     1741 tcacatccgg tttttccctg ttttatattc cccggaacat tttatttcca ggaatatccg
     1801 ggcctttatc ccgcatcata cacacagcca tgacaacccg ctcttctgca gtcatcattt
     1861 ccccgtggct tcgcggattt tctgttccgt cttatactgc gaaagcgca
//
LOCUS       DAZKGF010000360         1884 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZKGF010000360 DAZKGF010000000
VERSION     DAZKGF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.361"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            22..1695
                     /locus_tag="ACHOQV_005153"
     CDS             22..1695
                     /locus_tag="ACHOQV_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFP9633364.1"
                     /translation="MLNSSISLILASGLMMSTANAADDVKLKATKTNVAFADFTPTEY
                     STKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAIEAAKKSTP
                     TLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGIPLEETFLS
                     ELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQNRGFNYFM
                     GYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPFMIYLAYSA
                     PHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQYDNTIILF
                     TSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYNKLVSSMDFYP
                     TALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSHWWDEENTA
                     FWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLALYDINDLQQ
                     TKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
BASE COUNT          626 a          395 c          374 g          489 t
ORIGIN      
        1 gaacatcagt taaataaaaa actgcttaac tcatcaatat cacttatatt agcatctggt
       61 ctgatgatga gtactgcaaa tgcagcagat gatgttaagc tcaaagcaac aaaaaccaat
      121 gttgcctttg cggacttcac gccgacggaa tacagcacta aaggtaagcc aaatatcatt
      181 gttttaacaa tggatgattt agggtatgga caattgcctt ttgatgatag ttcatttgac
      241 cctaaaacga tggataaccg aaatgttgtt gatacataca aaataggtat tgataaagca
      301 attgaagctg caaaaaaatc cactccaaca ttagcggccc tgatggacga aggtgtaaaa
      361 ttcactaacg gctatgttgc acacggtgtc tctggccctt cacgtgccgc aatcctgacg
      421 gcgcgagctc ctgctcgctt cggtgtatat tcaaattctg atgcacagga tggtatccct
      481 ctggaagaaa cattcctgtc tgaattactt cagaatcatg gttattacac tgcagcaatc
      541 ggtaagtggc acttatcaaa aatcagcaac gtaggtgtgc ctgaagaaaa acaaacccgt
      601 gactaccacg ataacttcac caccttctcc gcagaagaat ggcaacctca gaatcgtggc
      661 ttcaactact tcatgggtta tcacgcatcc ggcgtttcac tttataactc cccgtcattg
      721 ttccgtaacc gtgaaaacat taaagctgaa gggtacatta gtgatcaatt aactgatgaa
      781 gcaattggtg taattgaccg tgcaagcaaa ctggaacagc catttatgat ttatctggca
      841 tacagtgcac cacacctgcc taacgatcag cctgcgccta aaaactatca ggagaaattc
      901 aacactggta gcaagactgc tgataactat tatgcgtcaa tttactccgt tgaccagggg
      961 gttaaaaaga tcctgaatac acttaaggaa aaaggtcagt acgacaacac catcattctg
     1021 ttcacctctg ataacggtgc tgtaattgat ggtccactgc cactgaatgg tgcgcaggct
     1081 ggttacaaag gccaaccgta tccgggtggt atgcatacac ctatgttcat gtggtggaat
     1141 ggcaagctga aaacgggtga ttataacaaa ctggtttcat caatggactt ctatccgacc
     1201 gctttagatg ccgcagatat caagatccct gcttctctga aactagatgg tgtatcatta
     1261 ttgccatatg tacagaaagg agctgataag aaatctgcag ctggcgaacc tcacaaatat
     1321 ctgtcctgga ttacctctta ttctcactgg tgggacgaag agaacactgc attctggaac
     1381 ggatatcata aattcgtgag aaaagagtct gatgattatc cgcacaaccc acatacagaa
     1441 gacttaagcc agtttgcata cactgtaaga agcaacgatt attctctggt ttacacctat
     1501 gcagagaata aactagctct gtacgatatc aacgatctgc aacaaactaa agatctctct
     1561 gcatcccatc ctgatgtagt taagaatatg aaggctgtta tgattgactt catcacgtca
     1621 agtcaaaaac cgatcagtga tatcaaccaa gccaaatttg agaatataaa aaaatcttta
     1681 actaacgtga aatgataata agcagggagt agatatatct actccctgga tatagggaaa
     1741 taaatcagtt agagcaataa ataatgaaca aacacgaaaa taaaaaatac tgttcatctg
     1801 acgagatttg gtgtacacaa cgtaaggagt taatcaaatc tgttcttgaa acaaagaaag
     1861 cttctgattt agaaaaacag agta
//
LOCUS       DAZKGF010000361         1884 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZKGF010000361 DAZKGF010000000
VERSION     DAZKGF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.362"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..216
                     /locus_tag="ACHOQV_005154"
     CDS             <1..216
                     /locus_tag="ACHOQV_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9633365.1"
                     /translation="AGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRAR
                     RATGLMTRDNYRTVNNITQGKHPEAKQ"
     gene            213..701
                     /locus_tag="ACHOQV_005155"
     CDS             213..701
                     /locus_tag="ACHOQV_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9633366.1"
                     /translation="MKLTLTLEADNVNVQALNMGRIVVDVDGVNLAELINVVCDNGHS
                     LRVVDESDRVSTDCTPPFAALTGIRCSTAHITETDNAWLYSLSHQTNDDCETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            713..910
                     /locus_tag="ACHOQV_005156"
     CDS             713..910
                     /locus_tag="ACHOQV_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9633367.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQVQLLHQNR"
     gene            995..1840
                     /locus_tag="ACHOQV_005157"
     CDS             995..1840
                     /locus_tag="ACHOQV_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9633368.1"
                     /translation="MSAITVSRPEVVTGHTDVICSTSVSHILAVRKTTLLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDWWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKECYEDEMFSIRYFKKGSAHITFKNQELVDRLNDIIARHYPR
                     MLPSL"
BASE COUNT          504 a          470 c          465 g          445 t
ORIGIN      
        1 gctggcattt cactgtgcga tgcagtgaac tttcttgttg aaaaatacgc actggtgcgt
       61 accgaccagc cggaattcag tgcctgtacc cgttctcagt taataaacag catcgatatc
      121 ctccgggctc gcagggcgac cggcctgatg acccgcgaca attacagaac ggtaaataac
      181 attacccagg ggaagcatcc ggaggcgaaa caatgaaact gactctgacg ctggaagcag
      241 acaacgttaa cgtgcaggcg ctgaacatgg gacgaattgt cgttgacgtc gatggtgtta
      301 atctcgctga actgattaac gtggtctgcg acaatggcca ctcccttcgt gttgttgatg
      361 aatctgaccg ggtctcaaca gactgcacgc caccatttgc tgcccttacc ggcatacgct
      421 gcagtaccgc acatatcacg gaaacggaca acgcctggct gtactcgctg tcacaccaga
      481 ctaatgacga ctgcgaaaca gaatggattc attttacagg cagcggatat ctgttacgta
      541 ccgatgcgtg gtcatacccg gttctgcggc ttaagcgcct gggcctgtca aaaacgttcc
      601 gtcgtctggt tgtcacactt acccgacgtt atggcgtcag tctcattcat ctggatgcca
      661 gcgctgaatg cctgccgggt ttacccactt tcaactggta accaggaaca acatgaaatc
      721 attaaccacg gaaacagcac tggatattct gattgcgtgg ctgcaggaca atatcgactg
      781 cgaatcggga attatctttg acaacgatga ggataaaacg gattcggcag cactgctgcc
      841 ctgcattgaa caggcgaggg aggatatcca taccctgcgc caggtgcagc ttctgcacca
      901 gaaccggtga gtcttactca ccagacttca ttccactgac gccagcctga acagggctgg
      961 cgttttcatt tatctgcgaa aaggaatatc gattatgtct gcaatcacag tctcccgtcc
     1021 ggaagtggtc accgggcata cggacgttat ctgctccacc tcagtcagtc acattctggc
     1081 tgtgcgaaag actacactgc tgcaaatcga cacacttatc cggcaactgg ctgaaatctc
     1141 ggtactgaca gaaagtattg gaggtaaaac tgctctggac tgggccatga aacaggattt
     1201 tcgctgcggc tgctggctga tggagaaacc tgaaaccgca atgaaagcta tcacacgcaa
     1261 tctcgatcgt gaaatctggc gtgacctgat gcaacgttcc gggatgcttt ccttaatgga
     1321 tgcacaggcc cgtgatacat ggtaccggtc actggaatat gataattttc cggaaatcag
     1381 cgaggcgaat atcctgagca catttgaaca actgcaccag aataaggatg aggtgtttga
     1441 gcgaggggtg atcaacgtct tcaggggact gagctggaat tacaaaacca attgcccctg
     1501 caaatttggc agtaaaatta tcgtcaacaa cctggtgagg tgggactggt gggggtttca
     1561 tcttatcacc gggcaacagg cagatcgact tgccgatctg gaaagaatgc tacacctgtt
     1621 cagcggcaaa ccgatccccg acaaccggga aaacatcacc cttcgtctgg atgatcacat
     1681 ccagtctgtt cagggtaaag agtgctatga agatgagatg ttcagcatca gatactttaa
     1741 gaagggctcc gcgcacatca cgttcaaaaa tcaagaactg gttgacaggc tgaatgacat
     1801 tattgcaaga cactatccga gaatgttacc ttcattataa agttctaaga atgaattcaa
     1861 ttttgcattt tagcttcatt ccct
//
LOCUS       DAZKGF010000362         1872 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZKGF010000362 DAZKGF010000000
VERSION     DAZKGF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.363"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..657
                     /locus_tag="ACHOQV_005158"
     CDS             <1..657
                     /locus_tag="ACHOQV_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633369.1"
                     /translation="MSMEIVMGKHSGKKSYERWVQGAKNRTVLFHYKNLITDLNQAHS
                     CRASLGAYCPFHGEILRKLDQRNEVQRVKLSIDVPFLEGLTPSELYKIRTDYESSFNA
                     FRSVLREAAYDIEAESDPYKRQLINQHFVERIWDEGLTDIEQKIQTYKRKSNKELFLN
                     IAPSLIGFIGAPNPVSLATGAFTILKEVFSITSHSSEIKSHPSYFLLMTSKGKNKFYD
                     "
     gene            complement(794..1312)
                     /locus_tag="ACHOQV_005159"
     CDS             complement(794..1312)
                     /locus_tag="ACHOQV_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633370.1"
                     /translation="MTILEEMEAEAWHILTTIYTYQRLMEGLTNSSTRYELANQLVAL
                     NALLEMLVIRLARLADKRKDVRSVSMFLKRGSYSASPEAVKLAAEKFLALTEPVLKIR
                     HEQIAHMKPGTLSSFEPRELPNEALRATEALIDLIDIARGHPQSYTYRVGSQEPAINL
                     RASVETSELVKI"
     gene            1668..>1872
                     /locus_tag="ACHOQV_005160"
     CDS             1668..>1872
                     /locus_tag="ACHOQV_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP9633371.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVH"
BASE COUNT          554 a          398 c          391 g          529 t
ORIGIN      
        1 atgtctatgg aaattgtcat gggaaagcat agcggaaaaa aaagttatga acggtgggtg
       61 cagggagcaa aaaatcgtac agttctcttt cattataaaa accttattac tgatcttaac
      121 caagcgcatt catgcagagc gtccctgggc gcatattgcc catttcatgg agaaattctt
      181 cgtaaactgg atcaaaggaa tgaagttcag agggttaaac tctctattga cgttccattt
      241 ctagaggggc ttactccaag tgaactctac aaaattagaa ctgactacga atcgagtttt
      301 aatgccttcc gctctgtgct acgtgaagct gcgtatgaca tagaagcaga aagtgaccca
      361 tataagaggc agctgataaa tcagcatttt gtcgagcgca tatgggatga aggattgacc
      421 gatattgagc aaaaaataca gacctataag cggaaaagta ataaagaact ttttcttaac
      481 attgcgccat cattgatagg ttttattggg gcaccaaatc cagtgtcgtt ggctacagga
      541 gcctttacga ttctaaaaga ggtttttagt ataacaagcc actcttcaga aataaagagt
      601 cacccttcat atttccttct catgacgtca aaaggcaaaa ataaatttta tgactgaagc
      661 agtaggaaat attgtgaaaa tatattcact taagcaaaaa ggttgagggg ctgccggatc
      721 tgttattaac ggggagctaa ttactttgcc gagtaaagca actcggcaaa gtagcataga
      781 cttgagtgaa ttattagatt ttaactagtt cgctagtctc tactgaagct ctcaaattaa
      841 tggcaggctc ctgtgagcca actcggtatg tgtaagactg agggtgacct ctggcaatgt
      901 caatgagatc gattagagcc tcagttgctc tcagagcttc atttggaagt tctcgaggct
      961 cgaagctact gagtgtgccg ggcttcatat gtgcaatctg ctcatgccta atctttaata
     1021 ctggttctgt gagtgcaagg aacttttcag ctgccaattt cacagcctct ggtgaagcag
     1081 aatacgagcc gcgtttcaaa aacattgata cagatctcac atctttcctt ttatccgcaa
     1141 gacgcgctaa acggatcacc aacatttcca acaatgcatt gagggctacc aactgattgg
     1201 caagctcata tcttgtactg ctattagtaa ggccttccat caaccgctga tacgtataga
     1261 tggttgttag gatatgccat gcttctgctt ccatttcttc aagaatcgtc ataaatactc
     1321 cacaatggtc tgatagttat catgcagata tctaaacaac caatttaaac taaatttata
     1381 attcaacacg tagcacatga tactgaatca ctacgtataa attagatact ttaggatttt
     1441 atttggacaa ccccgcccca gaagacaatt ctaccttagt gtatataagg accgcttatg
     1501 acatagagcg gactgtcaga tgaggtttag ttctgtacta atgagccgat acaaattagc
     1561 tgcatatacc tgcattccaa ataatttcag acagatatta tcgttgtgaa tcccccgcca
     1621 cctttccggt gcgcggggtt ttgtcttttt tcaccgggaa tacatgtatg aatccgtctg
     1681 atgccattga ggcaattgaa aaaccgctct cctccctgcc ttactcactt tcccgtcaca
     1741 tcctggaaca tctgcgcaaa ctcacccgtc acgaacccgt gattggcatt atgggtaaaa
     1801 gcggggccgg taaatcctca ctctgtaatg cactgtttca gggggaggtc accccggtca
     1861 gtgatgttca cg
//
LOCUS       DAZKGF010000363         1864 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZKGF010000363 DAZKGF010000000
VERSION     DAZKGF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.364"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..641
                     /locus_tag="ACHOQV_005161"
     CDS             <1..641
                     /locus_tag="ACHOQV_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFP9633372.1"
                     /translation="FLTQPSPAEQQLRALLTPPPDVLSADQIAQLSRTRSLLNHTSEW
                     IPLSGQQITRLTELPLAYNLQRSAQLLQQLRVLFPDNPRVQEMVDNWQKSVRSRALPE
                     EAMTGWNEGMTRLQQLAERLNRLDEQRGKYMTVSELKTEVFGIMQAFNRHIPAEEQLR
                     RYNEVRNQNGSEQQQKLTQDALTELLNRYWFLRHGDAGKKTINNKQDNQINI"
     gene            784..873
                     /locus_tag="ACHOQV_005162"
                     /pseudo
     CDS             784..873
                     /locus_tag="ACHOQV_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1278..>1864
                     /locus_tag="ACHOQV_005163"
     CDS             1278..>1864
                     /locus_tag="ACHOQV_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HFP9633373.1"
                     /translation="MAKMIPAFGPIDNNSYGEKTVYTLLKEGLSDDFTVIHSLPWLSA
                     ATRNLGMKLPPSGEIDFLVLHPVYGVLALEVKSGIYRVEGTVFVHIKTKKHVDIVKQT
                     RNNVHGLARWLGGATTLRLRIGYGFIFPDSYFDDKIINAAMVDASVKPFRGIFVDKSQ
                     LPEMAKHTIDIMQYWKHALGNNELGDERVQLIIKSV"
BASE COUNT          574 a          350 c          477 g          463 t
ORIGIN      
        1 agtttctgac acagccatca ccggcggagc agcaactcag ggcgctgctg acacccccac
       61 cggatgtgct ttcggcagac cagatagcac agctcagcag aacacgctca ctgttaaatc
      121 atacgtcaga gtggataccc ctttccggtc aacagataac gcgactgaca gaattacccc
      181 tggcatacaa cctgcagcgc agtgcgcagc tgctgcagca actgagggta cttttccccg
      241 ataatccccg ggtacaggaa atggtggata actggcagaa gagtgtaagg agccgtgcgc
      301 tcccggaaga ggcgatgacg ggctggaacg aaggcatgac ccgcctacag cagctggctg
      361 agcgcttgaa ccgtctggat gaacagcggg gaaaatacat gacagtcagt gaactgaaaa
      421 cagaggtgtt tggcatcatg caggctttta accggcatat cccggcagaa gagcagttac
      481 gtcgctataa tgaagtccgc aaccagaatg gcagtgaaca acagcaaaaa ctgacgcagg
      541 atgccctgac ggaactgttg aaccgctact ggtttctgag acatggagat gcaggtaaga
      601 agacgataaa taataaacag gataaccaga ttaatatctg acagatttta ttgccattat
      661 ttatccagaa gcgatgatgc cattaatgca aaatgacatc tgaattgatc acagattgga
      721 ccaatctgtg atcaaataga acagaatacg cagatgataa agcggctttc cctcgaagat
      781 tcacaacgtc atctggcaga atatgggtga gagttgcagg ctgatgcgta caaagcttac
      841 gatgcgttgt atgaaactgg ccgggtgaaa gatgtcatcc gtacggtgcg cgccagacta
      901 aacaggcatc atgatggaaa agtcgggtat cattcacagg catatttgtc agaatggtag
      961 cgtcagcggg ttttgattac accagttttg aagctcatac ttcctatgag gcactgcaga
     1021 aaacgaaagt gtcgtttagg cgctggggga ggggttaaaa aagtaacggc agaagtcacc
     1081 ctgaagttga ggcagtcaac tactgttagc tggctgctgc atgagagtgt ggagggagat
     1141 tgtatatatt ggttagtcag agtctgcttc ggaataagta tgagtaattt ttaaaatggg
     1201 cggaagttat ttttaattac tgggctatgt aacaaatgca gattcctgtc agaaacatgt
     1261 tataaggaaa tagtaatatg gccaaaatga ttccggcatt tggacccatt gataataata
     1321 gttatgggga aaaaacagtc tatacactgt taaaagaagg tctgtccgat gatttcacag
     1381 tgattcatag tctcccgtgg ttaagtgcgg cgacacgaaa tttagggatg aaattacctc
     1441 cgagtggaga aattgatttt cttgttctcc atcctgtata tggtgttttg gcgttagagg
     1501 taaagagtgg aatatatcgc gtagaaggta ctgtttttgt acatataaaa acaaaaaaac
     1561 atgtagatat agtgaagcaa acacgtaaca atgtccatgg tttagcccgt tggttagggg
     1621 gagcaacaac gttacggttg cgtattggtt atggttttat ttttcctgat agttatttcg
     1681 atgataaaat tataaatgca gcaatggtag atgcgtctgt taaaccattc aggggaatat
     1741 ttgttgataa aagccaactc ccggaaatgg caaagcatac tattgatata atgcaatact
     1801 ggaaacatgc attgggaaat aatgagttgg gggatgaacg ggttcaacta ataataaaat
     1861 ctgt
//
LOCUS       DAZKGF010000364         1857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZKGF010000364 DAZKGF010000000
VERSION     DAZKGF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.365"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..515
                     /locus_tag="ACHOQV_005164"
     CDS             <1..515
                     /locus_tag="ACHOQV_005164"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633374.1"
                     /translation="GSVYVNFFVVPEGDNMKGKIIPEKNKIKNLINQERDAIALWIKD
                     GMRLPDIYRLLKEKHPELTFSQNGFVFSLRRDASDLHENALFNRSTVAVFMQKQHSEM
                     TLMVNSGCLLKNVHEALFSHISYSVFLRYIVKLYPDLHYQAKLNRKGAVCGELHHEEN
                     ASVYQNDEVC"
     gene            complement(638..919)
                     /locus_tag="ACHOQV_005165"
     CDS             complement(638..919)
                     /locus_tag="ACHOQV_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633375.1"
                     /translation="MKTNKKGTTWHIFYRENSGAEVLLEIPSFRECLSVSKELMTPSN
                     YMICIEKNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
BASE COUNT          505 a          343 c          390 g          619 t
ORIGIN      
        1 ctggttctgt atatgtgaac ttctttgttg ttcctgaggg agataatatg aaaggcaaga
       61 ttataccaga aaaaaataag ataaaaaact taatcaacca ggagcgtgac gccattgcac
      121 tctggattaa ggatggaatg agactgccag atatttacag attactgaag gaaaaacacc
      181 cggaactgac gttttcacag aatggttttg tattttcact gagacgtgat gcaagtgatt
      241 tacatgaaaa tgcgcttttt aatcgcagta ctgttgctgt atttatgcag aaacaacatt
      301 ctgagatgac tctcatggtt aattctggtt gtttgctgaa aaatgtgcat gaggcgttat
      361 tcagtcatat atcttactcc gtgtttcttc gatatattgt gaagttatat ccagatttac
      421 actatcaggc aaaattaaac aggaaagggg ctgtctgtgg tgaattacat catgaggaga
      481 atgcttctgt ttatcagaat gacgaggttt gttaaaggtg ttttataaaa atagtggggt
      541 agtgctggca cagagatgtc ttacaggcat tctcccgcaa aaaatgaacc cgttgcttgt
      601 tatagtgcct tgcttaatac attgcctgaa tccggagtta ctttttaata attctgttaa
      661 tggttattag ctcacctaat atttcgaagt tatcaggtgg gcagttaatc catttgttag
      721 agccagcgat gatttccctg tcccagcgtt ttattctttc gccatttttt tctatgcata
      781 tcatgtaatt tgatggggtc attaattctt tggatactga aagacattcc cgaaatgatg
      841 gtatttcaag caaaacttcc gcgcctgaat tttcacggta aaaaatgtgc catgttgtgc
      901 cttttttatt agttttcacg tgcgtattct cataggataa atataaaacc ttgtaacatt
      961 caggaggtaa caggatttat tctgtaagaa caacagacag aacaatgata tctgttttat
     1021 gtatacgatg tagccagtga tttagtacat tgctggctat atcacttttc ccattttgtg
     1081 ataagtctgc tttgaaacga tggtgtccct cctcctgtat tgtgaaagtt gccacatttt
     1141 tttcttcata tccctgaaca tccccgtcgt gccttatata attgactcca ttaagaagtt
     1201 gtcagaataa ttatttctga ttgcaacgga gttaatttca gtgatggtaa agatatccgt
     1261 ggcaccataa cgcatttttc atcatcagta tagtggtatg cgtgaacata tatggtggca
     1321 ttttgcctga tgttgttaag gcttttcagc aggtctacag tcaactccat cgaggtatcc
     1381 ttcaggttgt tatcattcgt tgatgatgcg atctgttgaa ggcgttggcg acaattaatt
     1441 ctttttctct tctgaaaaat ttacggaata caaaaaagat ctgtttttaa ctgattgtta
     1501 ctttctgaag atattctctg ctccatttgc tttccgggta agacgtatgg ctggttatgg
     1561 tatggtcgga ctgcagccac agggggatga ctgtaatacc tctgatcacc acagtcagtc
     1621 atctgtaaat gttaaatacc tcacagttca gtcagtggat tattgtcggt cttacgggat
     1681 atttgttaaa aaaatactgc atggtgaatc cccctgagcg gcggggcata tcagtatcgt
     1741 aggtgtttgg tcacatccct tatctgtgcg ggttcaggtg ctgatactga actcaccggg
     1801 aggcacccgg caccatgcta cataacactc cattacgcta cactcgcccc tctccgg
//
LOCUS       DAZKGF010000365         1830 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZKGF010000365 DAZKGF010000000
VERSION     DAZKGF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.366"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1353
                     /locus_tag="ACHOQV_005166"
     CDS             <1..1353
                     /locus_tag="ACHOQV_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633376.1"
                     /translation="ETSGLEIILPDNAAYTDRPVPHFTHSGTGYELLASLGRIFSVPD
                     YIWHPLPDGGIMAAAAAQGMFSGRPVTIPHEFSQASTGGHSMTLPMIQTLRPGVEVNG
                     QRLTMVMLEDDNMTITWTPRNKATGAPLQKSPFRRQAEKAFPELASGLHLPKLARVEA
                     PTEAVSAGNIADPFRPRYAVNLQLLDENGNPAADTPVYNAVPLPVPMAGSESGMFQFP
                     PPGTLVEVGFVEGRADKPFIRQIMAEGHNLPDVKPGEQLQQQRDGVSQRVTVAGDWER
                     QTDQRIQETSRERVVSADEETRSLTSRETTIQATDTTTVLGVSTLQAGAVTHITEENY
                     SISTGGHMMVVAGTCERDVEGDTLDTVGGSLTEKIKGVRHSIALAQKLVGDTVKLGNE
                     GINVLTMLTDLADVVEELADITASHTHPKTGTSPQAAQFSQVAQECRQLKNKYSPIIE
                     "
BASE COUNT          445 a          550 c          512 g          323 t
ORIGIN      
        1 gagaccagcg ggctggaaat catcctgccg gacaacgcgg cttacactga cagacccgtt
       61 ccgcatttca cgcacagcgg cacgggctac gaactgctgg ccagtctggg gcgtattttc
      121 tccgtaccgg attacatctg gcatccgtta ccggacggcg gcattatggc cgctgccgcc
      181 gcgcagggga tgttttccgg tcgccccgtc accatcccgc acgagttcag ccaggcatca
      241 acgggcggac acagtatgac cctcccgatg attcagacgc tgcgaccggg tgtggaggtg
      301 aacggtcagc gcctgacgat ggtcatgctg gaagatgaca acatgaccat cacgtggaca
      361 ccgcgcaata aagccaccgg cgcccccctc cagaagtccc ctttccgtcg ccaggctgaa
      421 aaggccttcc ctgaactggc atcagggctt cacctgccca aactggcacg tgttgaggcg
      481 ccgaccgaag ccgtcagcgc gggcaatatt gccgacccgt tcagaccccg ctacgcggtg
      541 aatctccagc tgcttgacga gaacggcaac ccggcagcgg atacgcccgt ttataacgcc
      601 gttcccctcc ccgttcctat ggcgggcagt gagtccggca tgttccagtt cccgccaccc
      661 ggaaccctgg tggaagtggg atttgtggaa ggccgggcgg ataagccctt catccgccag
      721 ataatggcag agggccacaa tctgcccgac gtgaagccgg gcgagcagtt gcagcaacag
      781 cgcgacggcg tatcgcagcg cgtgacggtc gccggagact gggagcgcca gacagaccag
      841 cggatacagg agacatcacg cgagcgcgtg gtgagcgcag acgaggaaac gcggagcctg
      901 accagccggg agaccaccat acaggcaacc gacacaacca ccgtcctggg cgtatccacg
      961 cttcaggctg gcgcagtcac ccatatcact gaagagaact acagcatcag cacgggcggg
     1021 cacatgatgg tggttgccgg tacctgtgag cgcgacgtgg aaggcgacac actggacacc
     1081 gtgggcggca gccttaccga aaagattaag ggcgtccgtc acagtattgc gctggcgcag
     1141 aagctggttg gcgatacggt gaagctggga aacgagggca ttaatgtact gaccatgctg
     1201 acggaccttg ccgatgtggt ggaagagctg gccgatatta cggcatcaca tacccacccc
     1261 aaaacgggca cgtctccgca ggccgcacag ttcagccagg tcgctcagga gtgccgccag
     1321 ctaaaaaata aatattcgcc aataatcgaa tagttattca ttttaacagg ccgcgcaatg
     1381 tggccttttt tattcccccg ataataaaaa atattcatca caggacatca cccgccacga
     1441 aaaaaccgcc cgcagcagaa aaaaagatgg acgaaacgcc ccacgtaatc cccacgtaaa
     1501 acgtcgcgta acgccacgta acgaagagat aaccccgcca gcccgattcg atcccctgac
     1561 atcggaaaac gatttctgcg ccccaaaatc gccgcaaatg gccggaaatt ttcgggggcg
     1621 acgtaacgcg taaagtgacg taatcggcgc taccccgcac acgcctccgg tatttgtgta
     1681 cgataaattt tgcaaatcga aaggggtgca aaacaccacg ccagggcgcg ccattactga
     1741 ggttttccct tctgaatggt tttgcacttt gtgcaaggtt ttgcaaaagt gtgcaaaaac
     1801 tccggctttc tgaggatccc gaaaaatttt
//
LOCUS       DAZKGF010000366         1820 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZKGF010000366 DAZKGF010000000
VERSION     DAZKGF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.367"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1615
                     /gene="espI"
                     /locus_tag="ACHOQV_005167"
     CDS             <1..1615
                     /gene="espI"
                     /locus_tag="ACHOQV_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HFP9633377.1"
                     /translation="TGTPVNSDDAAFLPTINMADGGFKLMSDSSVLKARDQASVVGDI
                     ISDKQATISFGTESGKEGILSEKASRGLAVGLLSGFNTAYRGAIHAPSASATVNNTWW
                     QLTGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAYAMRTDLKNADKLVV
                     NQKLSGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSEQAIGFSNVTPVITA
                     IDAGDKTTWNLTGYRMAENPAATQSASGLASVGYKSFLSEVNNLNKRMGDLRDINGEA
                     GAWARIMSGTGSAGGGFSDNHTHVQVGVDKKHELDGLDLFTGFTVTHTDSSASADAFK
                     GKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYSTHSWYAGAEA
                     GYRCHVTEDTWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDRDYNPLIGRTGVDVGKS
                     FSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSVGLNAEIRDN
                     VRFGLEFEKSAFGKYNVDNAVNANFRYSF"
BASE COUNT          493 a          413 c          475 g          439 t
ORIGIN      
        1 taccggtaca ccagtgaatt cagatgatgc tgctttttta ccgaccatca atatggctga
       61 tggcggattt aaactgatgt ctgattcatc agtactgaaa gccagagacc aggcatctgt
      121 tgttggtgat attatttctg ataaacaggc cacaatcagc ttcggaactg aatcaggtaa
      181 agagggcata ttgtctgaga aggcatcccg gggactcgcg gtaggattac tgagtggttt
      241 taatacggca taccgcggtg caattcatgc cccgtcagca tctgccactg tgaacaacac
      301 ctggtggcaa ttgacaggag actcctcact tcgctcgtta aaaaataccg gaagcatgac
      361 atattttaca ggaagtgcag cgaataaagc attccataca ctgacggttg atgagctgac
      421 gacgaatggc actgcgtatg ccatgcgtac ggacctgaaa aatgcggata agctggtagt
      481 aaaccaaaag ctgtcaggta aggacaatat tctgctggtt gattttctga acaaacccac
      541 cggagaaaaa ctggatattg aactggtgag tgcaccgggg aacagcagta aggatgtttt
      601 taaaggaagt gaacaggcaa taggtttcag caatgtcacg cctgttatta cagctataga
      661 cgccggagat aaaacaacat ggaacctgac cgggtacagg atggcagaaa atcctgccgc
      721 aacccaaagt gcctcaggcc ttgcatctgt ggggtacaaa tcatttttga gtgaggtcaa
      781 caacctgaat aaacgtatgg gtgacctgcg tgacatcaat ggtgaagctg gcgcatgggc
      841 acgtatcatg agcggaaccg gctctgccgg tggtggtttc agtgacaacc acacacatgt
      901 tcaggtcggt gtcgacaaaa aacatgagct ggacggactg gatttgttta ccggcttcac
      961 tgtcacacat actgacagca gtgcctctgc tgatgctttc aaaggtaaaa caaaatctgt
     1021 gggggccgga ctctatgctt ccgccatgtt tgattccggt gcctatatcg acctgattgg
     1081 taagtatgtt catcatgata atgagtacac cgcaaccttt gccggactcg gaacccgtga
     1141 ttacagtacg cattcatggt atgccggtgc tgaagcaggc taccgctgtc atgtcactga
     1201 ggatacctgg attgagccac aggcagaact ggtttacggt gctgtatccg gtaaacagtt
     1261 tgcatggaag gaccagggga tgcatctgtc catgaaggac agggactaca atccgctgat
     1321 tggtcgtacc ggtgtggatg tgggtaaatc cttctcaggt aaggactgga aagtgacagc
     1381 ccgtgccggt ctgggctacc agttcgacct gctggctaac ggcgaaaccg tattgcggga
     1441 cgcatcaggt gaaaaacgta tcaaaggtga aaaagacagc cgtatgctga tgtccgttgg
     1501 cctgaatgca gaaatcaggg acaacgtccg ctttggactg gagtttgaga aatccgcctt
     1561 tggtaagtac aacgttgata atgctgtcaa cgctaacttc cgttactcgt tctgatactc
     1621 cgtattcaaa agggcctgag ggccctttat gctcaggacc tgccgcccgg gtatacattt
     1681 ccggagtatc agtcgatttg tttacttaac cgccatgacc aaagccggca gtaatgtcgg
     1741 ctttttacgt ccatatattc cactcatccg gaactgtgac acagaacctg aatacaggat
     1801 tgatcaccgg tgcccacaaa
//
LOCUS       DAZKGF010000367         1803 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZKGF010000367 DAZKGF010000000
VERSION     DAZKGF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.368"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            275..1195
                     /locus_tag="ACHOQV_005168"
     CDS             275..1195
                     /locus_tag="ACHOQV_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFP9633378.1"
                     /translation="MSRKSQRSRVEVFRKDFITLARECGGSYTTKADRVRIAKYFLNY
                     LRENGIKLRDTHSINTRHFQGYLLSRKVQGISHRTIQNERAVIRAILQKDGRYKLAAP
                     DNPLLSNETLGLTNTCRDGKKIPLPPEEFRKAFTEVEKKNPGVAAVMQLSYVLGLRTK
                     EAVQSCKSVKSWMRALESGHDSLLIVFGTKGGRPRDTIITDREVVRQALCYAEKIMNE
                     QSGKLIDRPNIKQAIDVYRYHVRKVGLTGEKSPHSMRYHFSQEARSFYRKDGLDDKEI
                     YARVSMDLGHGDGRGRYVKQVYFKNGDIQE"
     gene            1207..1545
                     /locus_tag="ACHOQV_005169"
     CDS             1207..1545
                     /locus_tag="ACHOQV_005169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633379.1"
                     /translation="MMYHILMEIKRIRYIRDIVLNSDNDYTEIIVHYRCKTPLNEFDT
                     CAMICRYFESVYFEDESGETFFTPKKTVVELWSEMVGVIRCRPDSRSLSLKPGNEIII
                     PIIYEIEDKL"
BASE COUNT          549 a          302 c          448 g          504 t
ORIGIN      
        1 gatgtctgga ggcgtcagcg ctggctgacg gggagtaaaa catcgccctg gtgcacaggt
       61 agcgaactcc tgtaaaaact caacatgtgc acgctctgct acatagacat catacgagcc
      121 aggaaggcga gcaaccgcgt tacagagccg tccgaaatga cgtaaaacta acaatatcaa
      181 tgtattagtc tgtttattcg ggaagactgt ttgtgcagtt gttcagtata agcggatgga
      241 tttctgtatc cgtatatcac caggagaaaa atgtatgagc agaaaatcgc agcgttccag
      301 agttgaggtg tttcgcaagg atttcattac ccttgcccgg gagtgtggcg gcagttatac
      361 gacaaaagcc gacagggttc gtattgcaaa atattttctg aattatttac gggaaaacgg
      421 aataaaattg cgtgacactc acagtatcaa tacgaggcat tttcaggggt atcttctgag
      481 cagaaaagta caggggattt ctcaccggac tattcagaat gaaagggcgg tgataagagc
      541 tattttacag aaggacggac gttataaatt agccgctcct gataatcctt tactgagtaa
      601 tgagacactc ggtctgacaa atacatgccg ggatggaaag aaaataccgt tgccaccgga
      661 ggaattccgg aaggcattta cagaggtgga aaaaaagaat cctggtgttg cggccgtaat
      721 gcaactttct tatgtactgg gtttacgaac gaaggaggcc gtccagtcgt gtaaatccgt
      781 taaatcgtgg atgagggccc tggaatcagg tcacgattct ctgttgattg tatttggcac
      841 aaaaggtggg cgcccccggg atactattat taccgatcgt gaagttgtca ggcaggctct
      901 atgttatgct gagaaaataa tgaatgaaca gagtggaaaa cttatcgatc gtccaaatat
      961 aaaacaggca atagatgttt atcgctacca tgttcgtaag gttggattaa ccggagagaa
     1021 atcacctcac agtatgcgtt accatttttc tcaggaggcc aggagttttt atcgaaaaga
     1081 tggcttggat gataaagaga tatatgcccg ggtttctatg gatttaggcc atggggacgg
     1141 tcgggggcgt tatgttaaac aggtttactt taaaaacggt gatattcagg aataatgcag
     1201 gaggatatga tgtatcatat attgatggaa ataaaaagga ttcgttatat cagagatatc
     1261 gttctgaata gtgataatga ttatacggaa atcatagtgc attaccgctg taaaacgcca
     1321 ctgaatgaat ttgatacatg tgccatgatc tgccgctatt ttgagtcagt atattttgaa
     1381 gatgaatccg gggaaacttt ctttacgcct aaaaaaacgg ttgttgaact ttggtcagaa
     1441 atggtggggg tgattagatg tcgtcctgac tccaggagtc tctctcttaa accaggtaat
     1501 gaaatcataa ttccaattat atatgagata gaagataaac tttgatcagg taaatatgat
     1561 cctgatgagt ttgatacgga agtgtttgta agaacgtggt taggtgatct ttttgatcaa
     1621 gatggcaata tattgcctca atgattggat tatatatgaa agataatggt aaaaataact
     1681 cctgaacatt cagaagtttt gatgtggcag gttttgactg ggtgatgcca gatggactgt
     1741 atttggacaa caacttgaca ttcatctttt gtgatttaaa aatatacagg cttatatgtc
     1801 gct
//
LOCUS       DAZKGF010000368         1786 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZKGF010000368 DAZKGF010000000
VERSION     DAZKGF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.369"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..129
                     /locus_tag="ACHOQV_005170"
     CDS             <1..129
                     /locus_tag="ACHOQV_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP9633380.1"
                     /translation="EMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKENHSRYVPG"
     gene            93..1181
                     /gene="tssG"
                     /locus_tag="ACHOQV_005171"
     CDS             93..1181
                     /gene="tssG"
                     /locus_tag="ACHOQV_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP9633381.1"
                     /translation="MERESQPVRTGLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIGNRSGLGAGNRIRLSQRAPLGKTTGEACSRLLMTLETADPNEAEGWLPGGVLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPPAEDGHYRFE"
     gene            1217..1741
                     /gene="tssJ"
                     /locus_tag="ACHOQV_005172"
     CDS             1217..1741
                     /gene="tssJ"
                     /locus_tag="ACHOQV_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP9633382.1"
                     /translation="MVTVIRWWVPMFIACGLLAGCGLTQTVTDGTISITKSIFYKKIK
                     TLHLDFTPRTAINTDGAQTPLATMVRVYQLRDRKAVDVAGYQMLLRNADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
BASE COUNT          376 a          503 c          531 g          376 t
ORIGIN      
        1 gagatgctgc accgtttctt tgcgctctat gctgatgttc accttttcaa ccagctcaca
       61 ctggtactgc aacccacagg gaaacgactg agatggaaag agaatcacag ccggtacgta
      121 ccgggctgac gcaggccctg gcccttaaca acgatatctg gcgggccagt ttttatcgtt
      181 tctgccagct gctggagcag gaaaatccgg acgggccaaa actgggcacg accagccatc
      241 cgggcgatga tccggtgcga ttcagaccct ggcccgggat gggattcccg gtcagcaccc
      301 tgaaagcggt ggagatcgac gaagaccgtc cgacattacc cccaacaatc cgcacgacat
      361 ttctcggaat gtatggggtg gactcacctc tgccgacgtc ctggctggat gatattacgc
      421 agcgccgtga aggacatgag gcactcacct catttctgga tattttcagc catcgcatca
      481 ccacgcagta ctaccggatc tggcgaaaat atgcgtaccc ggccactttt gaggaggggg
      541 ggcgcgatgc cacatcgcag tgtctgctgg ggctggtcgg gctcggtata cccggcaccg
      601 ctgaacaggt ggcaacgccg gtttcccgtt tcctggcctt actgggtgcc atgcgattac
      661 ccacccgcaa tgctgaaggc atccgggctc tggtgagtct gctggcaccg gatacccgcg
      721 cacttatcac cgaacccgat ccggttaagg tccatatcgg taaccgcagt ggactgggtg
      781 ccggaaaccg catccgtctt tcgcagcggg cgccgctggg taaaacgaca ggggaagcat
      841 gcagccggct cctgatgaca ctggaaacag cagatccgaa cgaagcggaa ggctggttgc
      901 ccggtggtgt cctccatacc gatttgctgg tcctgctgcg tgtttatctc ggctaccgca
      961 gtgacgtccg gctgcgactc actgtccccg tgcggttgtt gcctgagccc cgcctgggca
     1021 aaggcaggcc tgttcagctg ggccgtaccg gtttgctggg gctgaaagcg ggcaagctca
     1081 gcaatggccg ggagagtctg acggtcagtc tgggctgcta cgaagggttg aaatgtgctg
     1141 cgctaccgcc tgctgaagac ggacattatc gcttcgaata atcacctgtt ctctctctca
     1201 ataaaggagc gcctgaatgg taacagtaat ccgctggtgg gtgccgatgt ttattgcctg
     1261 tggcctgctc gcaggctgcg gtctgacgca gaccgtcacg gacggcacga tcagcatcac
     1321 gaaatccatc ttctacaaaa aaatcaaaac gctgcatctg gattttaccc cacgcacggc
     1381 cattaacact gacggggcac agacgccact ggcaaccatg gtgcgggttt accagctcag
     1441 ggatcgtaaa gccgttgatg tcgccggtta ccagatgctg ctgcgtaatg ccgatacggt
     1501 acttaaggac gacgtgctgg cctcaaaaga gctgctggtg atgcccggcg gcagcgtgac
     1561 gctcaatatg cccatggatg aagatgcgca gtttgtggct gtggtgggat tgtttaaccg
     1621 gccggatcag aaggacaatc gctggcgcct ggtactgacc cgtgatgacc tcaatccgga
     1681 taagccccgc accattgaac tgggtgatgg ctggctcagc cttgtgccgg taaaggagtg
     1741 acgtgatgga taaccggtca gcgttttttg acgggcagac ccgtta
//
LOCUS       DAZKGF010000369         1784 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZKGF010000369 DAZKGF010000000
VERSION     DAZKGF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.370"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..129
                     /locus_tag="ACHOQV_005173"
     CDS             <1..129
                     /locus_tag="ACHOQV_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP9633383.1"
                     /translation="EMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKENHNLHVPG"
     gene            93..1181
                     /gene="tssG"
                     /locus_tag="ACHOQV_005174"
     CDS             93..1181
                     /gene="tssG"
                     /locus_tag="ACHOQV_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP9633384.1"
                     /translation="MERESQPARTGLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIDNRSGLGAGKRIRLSQRAPLGKTTGEACSRLLMTLETADPDEAEGWLPGGVLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPSAEDGHYRFE"
     gene            1215..1739
                     /gene="tssJ"
                     /locus_tag="ACHOQV_005175"
     CDS             1215..1739
                     /gene="tssJ"
                     /locus_tag="ACHOQV_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP9633385.1"
                     /translation="MVTVIRWWAPMFIACGLLAGCGLTQTVTDGMISITKSIFYKKIK
                     TLHLDFTPRTAINTDGAQTPLATMVRVYQLRDRKAVDAADYQMLLRRADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
BASE COUNT          378 a          497 c          531 g          378 t
ORIGIN      
        1 gagatgctgc accgtttctt tgcgctctac gctgatgtcc accttttcaa ccagctcaca
       61 ctggtactgc aacccacagg gaaacgactg agatggaaag agaatcacaa cctgcacgta
      121 ccgggctgac gcaggccctg gcccttaaca acgatatctg gcgggccagt ttttatcgtt
      181 tctgccagct gctggagcag gaaaatccgg acgggccaaa actgggcacg accagccatc
      241 cgggcgatga tccggtgcga ttcaggccct ggcccgggat gggattccct gtcagcaccc
      301 tgaaagcggt ggagatagac gaagaccgtc cgacattacc cccaacaatc cgcacgacat
      361 ttctcggaat gtatggggtg gactcacctc tgccgacgtc ctggctggat gatattacgc
      421 agcgccgtga aggacatgag gcgctcacct catttctgga tattttcagc caccgcatca
      481 ccacgcagta ctaccggatc tggcgaaaat atgcgtatcc ggccactttt gaggaggggg
      541 gacgcgatgc cacatcgcag tgtctgctgg ggctggtcgg actcggtata cccggcaccg
      601 ctgaacaggt ggcaacgccg gtttcccgtt ttctggcctt actgggtgcc atgcgattac
      661 ccacccgcaa tgcggaaggc atccgggctc tggtgagtct gctggcaccg gatacccgcg
      721 cacttatcac tgaacccgat ccggttaagg tccatatcga taatcgcagt gggctgggtg
      781 ccggaaaacg catccgtctt tcgcagcggg cgccgctggg taaaacgaca ggggaagcat
      841 gcagccggct cctgatgaca ctggaaacag cagatccgga cgaggcggaa ggctggttgc
      901 ccggaggtgt tctccatacc gatttgctgg tcctgctgcg cgtttatctc ggctaccgca
      961 gtgacgtccg gctgcgcctt actgtccccg tgcggttgtt gcctgagccc cgcctgggca
     1021 aaggcaggcc tgttcagctg ggccgtaccg gtttactggg gctgaaagcg ggcaaactca
     1081 gcaatggccg ggagagtctg acggtcagtc tgggttgcta cgaagggctg aaatgtgctg
     1141 cgctaccgtc tgctgaagac gggcattatc gcttcgaata atcacctgtt ctctctcaat
     1201 aaaggaacgc ctgaatggta acagtaatcc gctggtgggc gccgatgttt attgcctgtg
     1261 gcctgctcgc aggctgcggt ctgacgcaga ccgtcacgga tggtatgatc agcatcacga
     1321 aatccatctt ctacaaaaaa atcaaaacgc tgcatctgga ttttaccccg cgtacggcca
     1381 ttaacactga tggggcacag acgccactgg caaccatggt gcgggtttat cagctcaggg
     1441 atcgtaaagc ggttgatgcc gccgattacc agatgctgct gcgccgggcc gatacggtac
     1501 ttaaggacga cgtgctggcc tcaaaagaac tgctggtgat gcccggcggc agcgtgacgc
     1561 tcaatatgcc catggatgaa gatgcgcagt ttgtggctgt ggtgggactg tttaaccggc
     1621 cggatcagaa ggacaatcgc tggcgcctgg tactgacccg tgatgacctc aatccggata
     1681 agccccgcac cattgaactg ggtgatggct ggctcagtct tgtgccggta aaggagtgac
     1741 gtgatggaga accggtcagc gttttttgac ggacagcccc gtta
//
LOCUS       DAZKGF010000370         1751 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZKGF010000370 DAZKGF010000000
VERSION     DAZKGF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.371"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            357..565
                     /locus_tag="ACHOQV_005176"
                     /pseudo
     CDS             357..565
                     /locus_tag="ACHOQV_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            552..741
                     /gene="tnpB"
                     /locus_tag="ACHOQV_005177"
                     /pseudo
     CDS             552..741
                     /gene="tnpB"
                     /locus_tag="ACHOQV_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(744..932)
                     /locus_tag="ACHOQV_005178"
     CDS             complement(744..932)
                     /locus_tag="ACHOQV_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP9633386.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            1467..1608
                     /locus_tag="ACHOQV_005179"
                     /pseudo
     CDS             1467..1608
                     /locus_tag="ACHOQV_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          459 a          435 c          375 g          482 t
ORIGIN      
        1 tattccactc tgttcgttca cggactggca tcgaagtctg aggtgtactg gcaatagcgg
       61 atacagtcat ttgttctttt tttaagcatt catccgatga tacttttcct gaatgctacc
      121 ggagtggtat tcccctgccg tgagggaggg cgctgacggt tataaaaatc ctgatatata
      181 cccattcccg tatgacagcg atggcgtcac atctctgttc taccatcttc acagaaatat
      241 ttcgttcatt agcattccgg tattcgtata accaccttcg gtggagaaag aataaacaat
      301 ccctggaatg cgatgagcgc ttgaccagaa aagcatcata ttgcttcatg gcgcatcagc
      361 gaccacagga tgccgcaaaa gccgaaaaac ggaccagagc gcctgcaatc attcctcgcc
      421 tcagtatctg ttgcccatgg agtggccgat aacagatcat tgtcggatga gccttatccc
      481 cttccttccc ggtggtatcc ggatgtgctg ccagccttct cagttaaccg atgttttccg
      541 ggctttttgc catactgagg actgataacg tattcctggt cgtaaaaccc gtcaatatgc
      601 gccggggcat cgacaaaact gacacagtac gtccggaaag aacttaacgc tgcatggcac
      661 gacaatgctg cctttgtctt taccaacaag gctcgttccc gcataaaagt tctacgccgg
      721 gataaacatg gcgtatggct gcgtcaggcc ttcactactt cacaccggaa gtcttccacc
      781 ctgacacgaa tataccgtcc gggctctttc gcaggtgcaa acagtttttc acattcggcg
      841 aatgtcattt ctgaatcctg gtaacgcaac cactgaaccg gcatattacc gggtttgatt
      901 attgtcgcag ttattttata tcgtttcccc atatcgcctc tcatcctgac aaaaaaatta
      961 ccggctgagc catcactccg gtaaagcatg cgaaaaaaag tgagcgtctc agcaatggct
     1021 catgcgatga ttctgtacgg gcaccgatgg cacctcagaa tacaacaaag atatcagagt
     1081 aacatgtctc tctcgttatt tttcagagtt cttgacaccc tccttctgcc acattgtcaa
     1141 cgccagtgga gacttctgct gtcagttttt tcatccatac agagtgacat taattatcct
     1201 gaacaaaacg aaatatcctg aatatattta ttttctcctg gtgccggaga catgatgatg
     1261 tacaggctca ggtgcggtgt gaatgtaaaa tggtatgtta attattcatt tctgaatgca
     1321 aactgaatgc aaacttattc cgaattgaat tctcctgaca ccacatgata aaaatgagga
     1381 tataataaaa tacttctctt ctggatgtgc cattacctgc cgttgctgta atacggcggg
     1441 tattttttac tgacatatcg ggaagccagc agacgttcgt agctacatcc ccgaaagtgg
     1501 cacactgcaa actataatac gagtaaaaca tcgatcaccg tagctgatgt gttcaacgat
     1561 cgtgcactag cgttctgcga ttctcaggga ctgcctgttc tgagaatcgg cgagtccgcg
     1621 atcggatacg atgcgatttc gcagtgactc agggattgca tctgaaaacc ctccggctat
     1681 gtcggtaaat atttatttct atgaaccgta cgattcagat agcattcgcc cggccccgta
     1741 agaataatct c
//
LOCUS       DAZKGF010000371         1739 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZKGF010000371 DAZKGF010000000
VERSION     DAZKGF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.372"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1739
                     /locus_tag="ACHOQV_005180"
     CDS             <1..>1739
                     /locus_tag="ACHOQV_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFP9633387.1"
                     /translation="ASAGGSFTFGSMSGSASVSLSRDKMHSNHDSVQEQTGIFAGRGG
                     FDVTTGQHTQLNGAVIASTATADKNRLDTGTLGFRDIENRADYKVEHQSVGISTGGSI
                     GGQFAGNMANNLLVGANHEGHAESTTQSAVSAGNITIRDTKSQKQDVADLNRDAAHAN
                     QTLSPIFDREKEQQRLQQAQLIGEIGNQVADIARTEGQIAGEKAKRDPAALNQARAEL
                     EAAGKPFTEQDVAQRAYNNGMAASGFGTGGKYQQAIQAATAAVQGLAGGNLSAALAGG
                     AAPYIAEIIKQTTPDGAGRVAAHAVVNAALSLAQGKNALAGAAGAATGEVVGMIATQM
                     YGKPVSELSETEKQTVSTLATVAAGLAGGLVGDSGASAVAGAQSGKTTVENNALSFGD
                     GFESNAAAATSWNKYAVDNNLTPEQTQAGLDKIAKGDMPDSTNITKVIVDGYQDGVMI
                     AGAWYLGPAASAGKVLGGGLLGLAANSGYQVYDLNQPQNANKSWDYLGSATSFTTGMM
                     APGRGVLANTGIAMGGAFFTDGPNTASLAGAGIGAGLGGAFGKYAPTAVGKILGNDPV
                     PGFIYELGGGAVS"
BASE COUNT          428 a          405 c          572 g          334 t
ORIGIN      
        1 atgccagcgc cggaggcagc ttcactttcg ggtccatgag cggttcggcg agtgtgagcc
       61 tcagccggga taaaatgcac agtaaccacg acagtgtgca ggagcagacg gggatttttg
      121 ccggcagggg cggatttgat gtcaccaccg gacagcatac gcaactgaac ggtgcggtga
      181 ttgcgtccac ggcaacggca gataaaaacc ggctggatac cggtacactg ggcttccgtg
      241 atatcgaaaa tcgtgctgat tacaaagttg aacaccagag cgtgggtatc agcactggcg
      301 gcagcatcgg tggccagttc gcgggaaata tggccaacaa tctgctggtt ggtgcaaatc
      361 atgaaggcca tgcagaaagc accactcagt cggctgtgtc tgccgggaat atcaccatcc
      421 gggatacgaa gagccagaaa caggatgtgg cagacctgaa ccgtgacgca gcacatgcaa
      481 accagacgct gtcccccatc tttgacaggg aaaaagagca gcagcgactg cagcaggcgc
      541 aactcattgg tgagattggt aaccaggtgg cagatattgc cagaacggaa ggacagatag
      601 ccggtgagaa agcgaagcgg gacccggcag cactgaatca ggcccgcgca gagcttgagg
      661 cagcaggaaa accgttcacg gaacaggatg tggcgcagcg ggcctacaat aacggtatgg
      721 cggcctcagg ttttggaacg ggaggcaaat accagcaggc gatacaggcc gcaacggcag
      781 cagtacaggg gctggccgga ggtaatctga gtgcagccct ggcaggtggt gcagcaccgt
      841 atattgctga aattatcaaa cagaccaccc cggacggtgc gggacgtgtg gcagctcatg
      901 cagtagtgaa tgcagccctg tctctggcac agggtaagaa tgctctagct ggtgccgcag
      961 gcgcagccac cggcgaggtg gtgggaatga tagccacaca gatgtacggt aagccggtga
     1021 gtgaactgag cgagacagag aagcagacgg tctcgacact ggcaacggtt gctgccggcc
     1081 tggccggtgg tctggtggga gactcggggg cttcagcggt agccggcgca cagtcaggca
     1141 agacaacggt tgagaataat gcgctcagct tcggtgatgg ttttgaaagc aatgcagctg
     1201 cggctacatc atggaacaag tacgcggtgg acaataatct gacaccggaa caaacacagg
     1261 ctgggctgga taagatagcg aaaggtgata tgccggacag taccaatatc acgaaagtga
     1321 tcgtggatgg atatcaggat ggtgtaatga ttgccggggc atggtatctg gggcctgctg
     1381 cgtcagcagg taaagttctt ggtggcgggc ttcttggact ggcggccaat agtggttatc
     1441 aggtctatga tttgaaccag ccgcagaatg cgaataagtc gtgggactat ctgggaagcg
     1501 cgacctcatt tacaacaggc atgatggctc ccggtcgtgg tgtccttgcg aacacaggta
     1561 tcgcaatggg tggggcattc tttactgatg gtccaaatac agcttcactg gccggggctg
     1621 gtattggtgc tggtctgggt ggggcatttg ggaagtatgc ccccacagcc gtggggaaaa
     1681 tcttagggaa tgatcctgtt ccgggcttta tttatgagct tggcggtggt gctgtctct
//
LOCUS       DAZKGF010000372         1719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZKGF010000372 DAZKGF010000000
VERSION     DAZKGF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.373"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..474
                     /locus_tag="ACHOQV_005181"
     CDS             <1..474
                     /locus_tag="ACHOQV_005181"
                     /inference="COORDINATES: protein motif:HMM:NF026977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633388.1"
                     /translation="PLGLLGGEQTYRYVPNPCGYVDPLGLAITSIINKTVEFSRGPQT
                     YVSDLGHVTGSTARARNKAITAILKEDFAYLKLTYIPQYNPFMRTGIAKLWEGTQFGK
                     NSFSSRAALRDVIIHEELHHRWWKRGLNDHHPKGTEMEQKFYETIRRYKRMRGWL"
     gene            474..887
                     /locus_tag="ACHOQV_005182"
     CDS             474..887
                     /locus_tag="ACHOQV_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633389.1"
                     /translation="MFGDEFKTTIECPCCGKICSIAIGQTSHNGILAWYESIICHNCG
                     LRSESDDIGFPPEKIRLQLIDFEGLWNVRLENIKSQMETIKVIRKAFSIDTKEALRII
                     RNESKVIYSGTREEAIWLMSLLKHVGETPLIQIAR"
     gene            942..1103
                     /locus_tag="ACHOQV_005183"
                     /pseudo
     CDS             942..1103
                     /locus_tag="ACHOQV_005183"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
     gene            1134..>1719
                     /locus_tag="ACHOQV_005184"
     CDS             1134..>1719
                     /locus_tag="ACHOQV_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFP9633390.1"
                     /translation="MNVTTQGRRAIEYHYPDEHTVIRCILPPEDERDRHPGESLLKTT
                     YRYNAAGELTEVILPGDETLTFSRDEAGREVLRHSNRGFACEQGWNAASQLVTQRAGF
                     FPEETTWGGLLPSLVREYRYDSAGNVSAVTSREDYGRETRREYRLDRNGQVTAVTASG
                     TGLGYGEGDESYGYDSCGYLKAQSAGRHRISEETD"
BASE COUNT          478 a          374 c          474 g          393 t
ORIGIN      
        1 ccgctggggt tgctgggggg tgagcagact taccggtatg tgcctaatcc gtgtgggtat
       61 gttgatccgc tgggattagc tataactagt ataattaata aaacagtcga gttttctaga
      121 gggccacaga cttatgtttc tgatctcggt catgttacag gttctacggc cagagccaga
      181 aataaggcaa ttacagccat acttaaagaa gattttgcat atcttaaact aacatatatc
      241 cctcaataca atccgtttat gcgaacaggg attgctaaac tttgggaagg aactcagttt
      301 ggtaaaaata gtttttcgtc tcgtgcagca ttaagagatg ttattattca tgaagaatta
      361 catcataggt ggtggaagcg aggattgaat gatcatcatc ccaaaggtac tgagatggaa
      421 cagaaatttt acgagacgat tcgaagatat aagcggatga gagggtggtt atagtgtttg
      481 gtgatgaatt taaaacgact attgagtgtc cttgctgtgg aaaaatatgt tctatagcta
      541 tagggcagac gagtcacaat ggtatactcg catggtatga atcaattata tgccataatt
      601 gcggattacg tagtgaatca gatgatatag gctttccccc agaaaaaatt cgtctacaat
      661 tgattgattt tgagggttta tggaatgtta gacttgaaaa tataaaatca cagatggaaa
      721 ctataaaagt cattcgtaag gctttttcga ttgatacaaa agaagcactt cgaattatta
      781 gaaatgaatc gaaggtaata tactccggaa cgagagaaga ggccatctgg ttaatgagtt
      841 tattaaaaca tgtgggtgaa acccccttaa tacaaattgc tagataaatt ctatgagctg
      901 ggtacaacgg agtagcctgg cgttaccgct atgacgccga tggcctgtgt accgccaggg
      961 ttaacggtct ggaggaaacc atcctctaca gccgggacgc cgcaggccgc ctggcagaag
     1021 tcatcacgcc ggaaggcaaa acgcagtatg cgtatgacaa atccggcagg ctgacgggta
     1081 tcttcagccc ggacggcaca tcacagcgca ccggctatga cgaacgcggg cgggtgaatg
     1141 tcaccactca gggccgacgg gccattgaat accactaccc cgatgaacac accgtcatcc
     1201 gctgtatcct gccaccggaa gatgaacgcg acagacatcc cggtgaatcc ctgctgaaaa
     1261 ccacctaccg ctacaacgcc gccggagaac tgacggaggt tatcctgccg ggggatgaga
     1321 cgctgacgtt cagccgtgat gaggcaggac gtgaagtgct ccggcacagt aaccggggtt
     1381 ttgcctgtga gcagggctgg aatgcagcca gccagcttgt cacccagcgc gccggatttt
     1441 tcccggagga aaccacatgg ggcgggctgc tcccctcact ggtacgggag taccgttacg
     1501 acagcgcggg caatgtgtcg gctgtcacca gccgggaaga ttacggacgg gaaacacggc
     1561 gggaataccg gctggaccgg aacggtcagg tcacggcggt gacagcctca ggcaccgggc
     1621 tgggctatgg cgaaggcgat gagtcctatg gctatgacag ttgtggctac ctgaaggcgc
     1681 agtctgcggg caggcaccgg ataagtgaag agactgacc
//
LOCUS       DAZKGF010000373         1707 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZKGF010000373 DAZKGF010000000
VERSION     DAZKGF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.374"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1473
                     /locus_tag="ACHOQV_005185"
     CDS             <1..1473
                     /locus_tag="ACHOQV_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633391.1"
                     /translation="GFYYHIYNFLQKSYCPCLDYSGTESDEAAVKRYLREYLAELGFN
                     REDFRENGKMYELGKYQGTIRKGYGKRKSLMKQYIKACKNEYKKDYREKKLDKSELDR
                     ILNNIDKFYYAFYSLSILLDMQRKVKILDSIAYYLRVLIREGLWVHGLYGYAARYLYD
                     FNIFDTTPYARALLERFHEFESGPKGALTRYIVSLDDKSQEYIESLKDMVFNLSDKKS
                     YDDVYLENIINYFEQLQNARGYVTRCYMLLAVFIYLIRRNKLHKALRFYDESQKYELP
                     FGYLPGAFSVLRIALEIKVNREKIKHGSLFELLDYVKAYQDAFMDLRVVTDPAYNEDE
                     IQYDANNFTLMRVIKMYNSMLANISTKSDIQPPYITGLLDNVERALDKINILIDKERV
                     YDGETLAELITENKILSSRESKENLIGLFTGRHKYTLLQCIEKLGVLVDYVISPADDI
                     KNVMMLYGNNAENKNRRRLIYNALTIICGDDTKNNQSDPR"
BASE COUNT          554 a          258 c          367 g          528 t
ORIGIN      
        1 gggttttatt atcatatcta taattttttg caaaaatcat attgtccctg cctggattac
       61 tctggtacag aaagcgatga agctgccgtt aaacgttatc tccgggagta tcttgctgaa
      121 ctgggattta acagagaaga ttttcgggaa aacggaaaaa tgtatgagct gggaaaatac
      181 cagggaacta ttcgaaaagg gtacggtaaa aggaagagcc ttatgaaaca atatataaag
      241 gcctgtaaga atgaatacaa gaaggattac agagagaaga agcttgataa gagtgagctg
      301 gacagaatac tcaacaacat tgacaagttc tattatgcat tttattcatt aagtattttg
      361 cttgatatgc aacggaaggt aaagatactt gacagtattg catattatct cagagtgctg
      421 ataagagaag ggctttgggt acatgggttg tatggttacg ctgcacgcta tctttatgat
      481 tttaatattt ttgacacaac gccttatgca agagcattac ttgaacggtt tcatgagttt
      541 gagagtggcc ccaaaggagc attaactcgt tatattgtgt cacttgatga taaatcacaa
      601 gaatatatcg aatctttaaa agatatggtg tttaatcttt ctgataagaa aagttatgat
      661 gatgtatatc tggaaaacat tattaattat tttgaacagt tgcagaatgc ccgtggttat
      721 gtgaccaggt gctacatgtt actggccgtt tttatttatc tgattcggcg gaacaaactt
      781 cacaaagctc ttcggtttta tgatgaatcc caaaaatacg agttaccttt tgggtatctt
      841 cccggcgctt tttcagttct caggatagcg ctggagatca aagtaaacag ggagaaaata
      901 aagcatggtt cactgttcga attacttgat tatgtaaaag cttatcagga tgcatttatg
      961 gacttaaggg tcgtgacgga tcctgcgtat aatgaagatg aaatacaata tgatgcgaat
     1021 aattttactc tgatgcgtgt cattaaaatg tataattcga tgcttgcaaa tattagtaca
     1081 aagtcagata ttcagcctcc gtatattaca ggtcttctcg ataatgtaga gagggcgtta
     1141 gataagataa atattctgat agataaagaa cgtgtttacg atggtgaaac gttggctgaa
     1201 ttaattacag agaacaaaat attatccagt cgtgaaagta aagaaaacct gataggatta
     1261 tttacgggca gacacaaata tactttactg caatgtattg agaagcttgg cgtattggtt
     1321 gattatgtaa tatcaccggc tgatgatatt aaaaacgtca tgatgttgta tggaaataat
     1381 gctgaaaaca agaacagacg cagactgatc tacaacgcat taaccatcat ttgtggtgat
     1441 gatactaaaa acaaccagtc agaccctaga tgatattatc ggaacgtgtt tttggcatcc
     1501 gatgtttgta tatccctgct attgttgcta gtcggctagc aacaatagca gagggggcat
     1561 gcatgtctga aaaaataaaa aatcgcaaca cattgatttt tatcttgttt gcattttgca
     1621 aatactatct ctcgtttttg tgattagtgt catgttttat cgtctcgcgg ataaccgtga
     1681 ggagtgaaca gtaaccctga ttatatg
//
LOCUS       DAZKGF010000374         1656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZKGF010000374 DAZKGF010000000
VERSION     DAZKGF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.375"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1288
                     /gene="tssC"
                     /locus_tag="ACHOQV_005186"
     CDS             <1..1288
                     /gene="tssC"
                     /locus_tag="ACHOQV_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP9633392.1"
                     /translation="LIDHHIAELDDQISRQLDAVLHHPEFQKVESLWRGVKSLVDKTD
                     FRRNVKIELLDLSKDDLRQDFEDAPEIIQSGLYLQTYVAEYDTPGGEPIAALVSAWEF
                     DASAQDVALLKNISRVAASAHMPFIGSVGPAFFRKETMEEVAAIKDIGNHFERAEYIK
                     WNAFRETDDARYIGLVMPRVLGRLPYGPDTVPVRSFNYVEEVKGPDHHKYLWTNASFA
                     FAANMVRSFVTNGWCVQIRGPQAGGAVQDLPIHLYDLGTGNQVKIPSEVMIPETREFE
                     FANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDATANSRINARLPYIFLLSRVAH
                     YLKMIQRENIGTTKDRRLLELELNTWLRGLVTEMTDPGDELQASHPLRDGKVVVEDIE
                     DNPGFFRVKLYAIPHFQVEGMDVSLSLVSQMPKAKA"
     gene            1304..>1656
                     /gene="tssK"
                     /locus_tag="ACHOQV_005187"
     CDS             1304..>1656
                     /gene="tssK"
                     /locus_tag="ACHOQV_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP9633393.1"
                     /translation="MKIFRPLWSEGVLLSPQQFQQQAEWESFRSAGISALASPFPWGV
                     EQVEFNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADRDAVTVV
                     IALPHMQPGVANVEQ"
BASE COUNT          350 a          401 c          483 g          422 t
ORIGIN      
        1 gctgattgac catcatattg cagagcttga tgaccagatc agtcgtcagc tggatgccgt
       61 gcttcatcat ccggaatttc agaaagtgga atccctgtgg cggggcgtga agtctctggt
      121 ggataaaacg gacttccgcc gtaatgtgaa aattgagctg ctggatctgt ccaaagacga
      181 tctgcgccag gattttgagg atgcgccgga aattattcag agcggccttt atctgcagac
      241 ctatgtggct gaatatgaca cgccgggtgg tgaacccatt gctgccctgg tttcggcctg
      301 ggaatttgat gcatctgcgc aggatgtggc gttgctgaaa aatatttccc gggtggcagc
      361 atccgcacat atgccgttta ttggttctgt tggtccggct ttcttccgga aagaaaccat
      421 ggaagaggtg gctgctatta aggatattgg caatcacttt gagcgtgccg agtacatcaa
      481 gtggaacgct ttccgagaga cggatgatgc ccgctatatt ggtctggtga tgccccgtgt
      541 gctgggacgc ctgccgtatg gccctgatac tgtacctgtc cgcagcttta actatgttga
      601 agaggtgaaa gggccggatc atcacaagta tctctggacg aacgcgtctt ttgcttttgc
      661 ggccaatatg gtgcgcagtt ttgtcaccaa cggctggtgt gttcagatcc gcggtcctca
      721 ggccggtggt gcggtgcagg atttgccgat ccatctgtat gatctgggaa ccggtaatca
      781 ggtcaaaatt ccgtcagagg tgatgatccc ggaaacgcgg gagtttgaat tcgctaatct
      841 gggttttatt ccgctctctt actacaagaa ccgggattat gcctgtttct tctccgctaa
      901 ctccacccag aaaccggcac tgtacgatac gccggatgca acggcgaaca gccgtattaa
      961 tgcccgtctt ccctatattt tcctgctgtc ccgcgttgcg cattacctga agatgattca
     1021 gcgggaaaat atcggtacca ccaaagatcg gcgtctgctg gagctggagc tgaatacctg
     1081 gctccggggc ctggtgacgg agatgaccga tccgggcgat gagcttcagg catcgcatcc
     1141 gctgcgtgac ggaaaagtgg tggtggaaga tattgaagat aacccggggt tcttccgtgt
     1201 gaaattgtat gccattccgc atttccaggt ggaaggaatg gacgtcagcc tgtccctggt
     1261 ttcgcagatg ccgaaggcaa aagcgtaagg caggtgtcgg gaaatgaaga ttttccgtcc
     1321 gctgtggagc gaaggtgttt tgctctcccc tcagcagttt cagcagcagg ctgaatggga
     1381 gtcttttcgc agtgccggta tctctgcgct ggcatccccg tttccctggg gagtggaaca
     1441 ggttgagttt aatgacagtc ttctgtcgtc cgggctgatc cagattacgc agttacgcct
     1501 ctggctggaa gacggaacac ttattgatgc tcaaaggagc gatctgcctc cggcgccacg
     1561 tgagttagat gccggtcagc tggctgaccg cgatgcggta actgtcgtta ttgcacttcc
     1621 ccacatgcag ccaggcgttg ccaacgttga gcagga
//
LOCUS       DAZKGF010000375         1643 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZKGF010000375 DAZKGF010000000
VERSION     DAZKGF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.376"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(66..1490)
                     /locus_tag="ACHOQV_005188"
     CDS             complement(66..1490)
                     /locus_tag="ACHOQV_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFP9633394.1"
                     /translation="MFQRSKYDSAYVYALCCVAALAGLMFGYSTAVITGVILPLQQYY
                     QLTSTETGWAVSSIVIGCIIGALVGGKIADKLGRKPALLIIAVIFIVSSLWGAMSESF
                     MVFSISRIVSGFAVGMAGTASTMYMSELAPADIRGKALGIYNISVVSGQVIVFIVNYV
                     IAKGMPSDVLVEEGWKSMLFAQVIPSVTMLVITLFLPESPAWCARNNKGEKRSLKVLA
                     RIYRGVSATDVAAIFDSMRETEARENAAGREFVILKSSPVLRYILLVGCCIAVLQQFT
                     GVNVMNYYAPLVLQNSSTEEVMFQTIFIAVCNVIGSFIGMILFDRYGRIPIMKIGTIG
                     SIIGLLIASYGLYTHDTGYITIFGILFFMLLFAVSWSVGAWVLISEVFPEKIKGFGMG
                     LAVSLMWIANFLISLLFPVINDNTWLQETFGGAFSMWIFVVFNLVCYVFISRYVPETK
                     GIPLTQIEQLAGNKLREIQGKAMM"
BASE COUNT          563 a          432 c          271 g          377 t
ORIGIN      
        1 ggaaaatccc ggtaagatgc tcaacattac cgggcattac tgctgattgt gacacaatca
       61 gactattaca tcatagcttt accctgaatt tcacgcagtt tatttccggc aagctgctca
      121 atttgtgtta gcgggatccc ctttgtttca ggaacatagc gagaaataaa aacataacaa
      181 accagattaa agacaacaaa aatccacata gaaaacgctc cgccaaaggt ttcctgcagc
      241 caggtattat catttattac cggaaacaac agtgatatta gaaaattcgc aatccacatt
      301 aaactaaccg ccagccccat gccgaatcct ttaatctttt cgggaaacac ctcagaaatc
      361 agtacccatg ctcctacgct ccagctgaca gcaaacaaca gcataaaaaa taaaatccca
      421 aaaatggtga tatagccagt atcatgggta tacaaaccat atgatgcgat caacaaacca
      481 ataattgacc cgatagtgcc aatcttcatg attggtattc gtccatagcg atcgaacaaa
      541 atcatcccga taaaactacc gatcacatta caaacagcaa taaaaatcgt ctggaacatc
      601 acttcctctg tactgctgtt ttgtagcacc agaggtgcgt agtagttcat cacattgaca
      661 ccagtaaact gttgtaaaac agcgatacaa cagccaacca gcagaatata gcgcaacaca
      721 ggagagcttt tcaggataac gaattccctg cctgcagcat tctcccgggc ctccgtttcc
      781 ctcatactat caaaaatagc ggctacatcg gtagcgctaa ccccccggta gattctggca
      841 agcactttca gagaacgttt ttcccccttg ttattacggg cacaccatgc cggcgattca
      901 ggcaaaaata aagttatcac taacattgta acagaaggta tcacctgagc gaataacatg
      961 ctcttccagc cttcttcgac cagcacatca gagggcatcc ccttagcaat cacataatta
     1021 acaataaaaa caatcacctg accagaaact actgatatat tgtaaatacc cagagcttta
     1081 ccacgaatgt cagctggtgc cagttcagac atatacatgg tagatgcagt ccccgccatc
     1141 ccaacagcaa aaccactgac gatgcgcgaa atcgagaaaa ccataaaaga ttcactcatc
     1201 gccccccaca gtgacgagac gataaaaata actgcaataa tcagaagcgc aggcttacgc
     1261 cccaacttat cggcaatttt ccccccgacc agcgcaccaa tgatacaacc aatcacaatg
     1321 ctcgatactg cccacccagt ttcagttgaa gtgagctggt aatattgctg taaaggcagt
     1381 atcacccccg ttatcactgc cgtcgaatag ccaaacatca atccagccag tgcagcaacg
     1441 cagcacaatg cgtacacata ggctgaatca tacttgcttc tctgaaacat accccctccc
     1501 tgcctgttga ggaaatgaca tacgaacatt tattccatac ttaaacaaat cagctcaaat
     1561 attccacaac aaagatcaca aaatataaaa atttcataac tgtgaaaaat agtttgtagc
     1621 acaataatca tattgaaaat aca
//
LOCUS       DAZKGF010000376         1643 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZKGF010000376 DAZKGF010000000
VERSION     DAZKGF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.377"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(151..1542)
                     /gene="narK"
                     /locus_tag="ACHOQV_005189"
     CDS             complement(151..1542)
                     /gene="narK"
                     /locus_tag="ACHOQV_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFP9633395.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWV
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLSVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT          493 a          445 c          395 g          310 t
ORIGIN      
        1 gtattaagga gtattcccca tgggtaacat attaatggct cttaatgaaa accggcgaaa
       61 tacgccacac ttcataagga tgttgtaact aaatgacagg taaaaaaagc gcgacatcat
      121 gccgcgcaaa ggataatcaa atattaagat ttatttttta gaatgccgac catataccgc
      181 ccaggtaatc actacgcagg cgatatagaa aatcaaaaat actttcattg cgccgactgg
      241 cgaacccgtt aatgccagcg agctaccgaa cgctttcggg ataaagaagc caccaatcgc
      301 gccaatcgca gagatgaaac ccagcgccgc cgccgtgtcg gttgccgctt cacgcatcgc
      361 acgttcgtca gagccccctt ctgctttcac gcgatccatt gtcagtttac ggaagatcac
      421 ggaaatcatc tgaaaagtgg aaccactacc cagcccagct gtcaggaaca gcgccaggaa
      481 gactgcgaag aacgccatga agcttccgcc ctgtccgtca gtcggtaagg tcaggaacag
      541 caggccgctg aaaatcgcca tcaggataaa gttcaccagt gtgacacgag ttccgcccag
      601 acggtcagat aatgcaccac ctgcagaacg cgccagcgca ccaataaacg gcccgaagaa
      661 agcgtattgc agaatctgaa catccgggaa ctgcgttttt gacagcatcg caaagcccgc
      721 ggagaagccg atgaaggagc cgaaggttgc cagatacagc aggctcataa tccacagatg
      781 accccgtttg agtaccgaca actgctcctt gatggaggct ttcgaggtag caagatcgtt
      841 catgccaaac cacgccgcaa tggtgaagat ggcaaggaac ggcacccata cccaggacgc
      901 attcgccaga tacagctcag tcccatccgg ctgtttgaca ccctggctac caaatactgc
      961 gaaaatcgac agtgatacca ccagcggagc aaccaactgc atgacgctga cgcccatgtt
     1021 gcccagacca ccattcagac ccagcgcgcc accctgcttc tgtttcggaa agaagaagct
     1081 gatgtttgcc atactggatg cgaagttcgc gccagcaaag ccgcacagca gagagatgat
     1141 gatgaagacg ctataaggcg tggaggtatc ctgcacggca aaacccagcc agacgcaagg
     1201 aataatcaga ataccagtgc tgaacgccgt ccagcgacga ccaccgaaga taggaaccat
     1261 aaaggagtat ggaacacgta ataacgcgcc agaaaccgaa ggcagcgccg tcaacataaa
     1321 tagctgatcg gtcgtaaaat taaaaccgac tttcggtaag ttcaccgcaa cagcgctgaa
     1381 caacatccat acgcaaaacg ccagcagcag acagggaacg gaaatccaca ggttgcggct
     1441 ggcaatacgt tgaccgcgtt gttgccagaa cgcaggatct tccggtcgcc aatctgtaat
     1501 gacagctcca gtagcccttt cgggggcgga tgagtgactc atagacacct ctgatactcg
     1561 tttcgatgtc tgccacctta gtgtctgtag ctaaaggcaa tttgatgtaa atcaaacgat
     1621 aagataactt tatctatcat tga
//
LOCUS       DAZKGF010000377         1626 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZKGF010000377 DAZKGF010000000
VERSION     DAZKGF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.379"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(101..589)
                     /locus_tag="ACHOQV_005190"
     CDS             complement(101..589)
                     /locus_tag="ACHOQV_005190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9633396.1"
                     /translation="MFQLTPFNSTYDRTSFLCGSDALDRYIKEHVTQDIRRRIAACFV
                     AVDNDHHIIGYYTLSAASILLTDLPEHLRKKLPRYPTIPAIRMGRLAVSLSSKGTGLG
                     GALLADALYRAYTSEIAAYALIVDAKDDTAVAFYKHYGFMAFPESGRTLFMPLASLST
                     IK"
     gene            complement(595..876)
                     /locus_tag="ACHOQV_005191"
     CDS             complement(595..876)
                     /locus_tag="ACHOQV_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP9633397.1"
                     /translation="MPSATQTARLEARISTELHAMLKRAAEIQGRTMTDFIIAAVQDA
                     AQRAIEQTDIIRLSRTDQEIFAQALLSPTEPNDALKRAFKRRNNMLYTK"
     gene            complement(1294..>1626)
                     /locus_tag="ACHOQV_005192"
     CDS             complement(1294..>1626)
                     /locus_tag="ACHOQV_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFP9633398.1"
                     /translation="IGQSPGRELSARARQIALGVDGVQEVYNEIRQGTPVSPVIMAGD
                     AWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLGLVTEQEGRAAADVASRVSGVKRV
                     TTAFSYIR"
BASE COUNT          417 a          389 c          377 g          443 t
ORIGIN      
        1 acaatccggc ggcagatttc ctgccgccgt gttgcccgtt aacatttaac ttcgtgggct
       61 gacgttgcca caagcaccgt atttccctga tttattctgt ttattttatt gtggataatg
      121 atgccaatgg catgaataaa gttcttccgg actccgggaa cgccataaag ccataatgtt
      181 tgtaaaatgc gactgccgtg tcatcttttg catcaacaat aagcgcatat gctgcaattt
      241 cagatgtgta tgcacgatat agcgcatcag ctaaaagtgc tcccccaagc cccgttcctt
      301 ttgatgacag acttactgcc agtcgtccca tacgaatagc cggaatagtg ggatatcgcg
      361 gaagcttttt tctcaaatgc tcaggtaagt cagttaataa tatactggcc gctgataacg
      421 tatagtatcc gatgatatga tgatcgttat ctacagcgac aaaacaggcc gcgatgcgtc
      481 gtcgaatatc ctgtgtgaca tgctctttta tgtaacggtc cagagcatca gagccacata
      541 aaaatgaggt tctgtcatat gtgctgttaa atggtgtcag ttgaaacatt atatttattt
      601 tgtgtacagc atattattac ggcgtttaaa ggcccgttta agcgcatcat tcggctctgt
      661 tggtgacagt aatgcctgag caaaaatttc ctgatctgtc cgggacagtc ggattatatc
      721 ggtttgttcg atagcgcgtt gggccgcatc ctgaacggca gcaatgataa aatcggtcat
      781 agtgcgcccc tggatttctg cagcgcgctt cagcatggca tgtagttctg tgctgatcct
      841 tgcttccagt cgggcagttt gtgttgcaga tggcatgatt tgtacctcct gaatacactg
      901 tacggcaaat tgccgtattg tgcaaattac actctgacac cgggacggca gacagacatt
      961 ctgcctgaaa agaaaaatca gattcagaca aaactatccg ggggatgtta acgccacgat
     1021 ctttgaccat tgcatacaga taaaagctca gattatttac cagcagtgac atccggagta
     1081 caccgggatt ttatctgtga gtgaacatac ccgggcaaaa aaatcccccg aattcagggg
     1141 ggtgtcaaag aggaattatg tatattggtt attataatga gcacgcctcc ggtattccgc
     1201 gacgaatgtt cacgtggtgg catgaacggc ccggaaacgt gctcttctga tttttaacgt
     1261 aaatttacac taaaggcaac tctgatactg ccttcaccgg atataggaga aggccgtcgt
     1321 gacccgcttc actccgctca ccctactggc aacatctgct gccgctcgcc cttcctgctc
     1381 tgtaaccagg cccagtaaaa agacttcgct gttttctgtc accaccttga cactggatga
     1441 tttgacacgg gcacttgtga gtaactgaga acgaattttt gtcgtaagcc aggcatcacc
     1501 tgccattatg accggactga cgggtgtccc ctgccggatt tcattataga cctcctgaac
     1561 accatcgaca ccgagtgcta tctgacgggc acgggcagat aattcacggc ccggagactg
     1621 accgat
//
LOCUS       DAZKGF010000378         1612 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZKGF010000378 DAZKGF010000000
VERSION     DAZKGF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.380"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1515
                     /locus_tag="ACHOQV_005193"
     CDS             <1..1515
                     /locus_tag="ACHOQV_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9633399.1"
                     /translation="VRTTTSDLDIRIPYATDPAGNRLPDPELHPDSTLTVWPDNRIAE
                     DAHYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVE
                     SRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDHLTTVQTDTTRIQTV
                     YEPGSFTPLIRIETDNGEREKTQRRSLAEKLQQEGSEDGHGVVFPAELVRMLDRLEGE
                     IRADRVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLALISTEGA
                     TEWRGEYDEWGNLLNEENPHHLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGRYITQD
                     PIGLEGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSGDLISL
                     GGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKSLPTSGVSNSV
                     GVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACRTWQECWVN"
BASE COUNT          410 a          367 c          497 g          338 t
ORIGIN      
        1 gtgcgcacga ccacatcaga cctggatatc cgcatcccgt atgccaccga cccggcggga
       61 aaccgcctgc cggacccgga gctgcacccg gacagtacac tcacagtgtg gccggataac
      121 cgcatcgcgg aggatgcgca ctatgtctac cgccacgatg aatacggcag gctgacggag
      181 aagacggacc gcatcccggc gggtgtgata cggacggacg acgagcggac ccaccactac
      241 cactacgaca gccagcaccg cctggtgttc cacacgcgga tacagcatgg cgagccactg
      301 gtcgagagcc gctacctcta cgacccgctg ggacggcgaa tggcgaaacg ggtatggcgg
      361 cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta aaccggaggt gacgtggtac
      421 ggctgggacg gcgaccacct gacgacggtg cagaccgaca ccacgcgtat ccagacggta
      481 tacgagccgg gaagcttcac accgctcatc cgcatcgaaa cagacaacgg cgagcgggag
      541 aaaacgcagc ggcggagcct ggcggagaag ctgcagcagg aaggaagcga ggacggccac
      601 ggtgtggtgt tcccggcaga acttgtgagg atgctggaca ggctggaggg ggaaatccgg
      661 gcagaccgcg tgagcagtga aagccgccag tggctggcac agtgcgggct gacggtggag
      721 cggctggcgg cacagataga gccggtttat ttacctgagc gaaaaattca tctttatcac
      781 tgtgaccacc gggggctgcc gctggcgctc atcagtaccg aaggagcaac ggaatggcgt
      841 ggggaatatg atgaatgggg caacctgctg aatgaggaga acccgcatca cctgcaccag
      901 ccataccgtc tgccggggca gcagcatgat gaggaatcag gactgtacta taaccggaac
      961 cggtactatg acccgctaca ggggaggtat attacacaag accccattgg gctggagggg
     1021 ggatggaacc tgtataatta cccactgaat ccgataataa ggatggatcc tttgggtttg
     1081 tataatttat atcaattatt atatgatgtt tggcatgatg attcatatgg aacatcatca
     1141 attgatatta ctggcagtgg agatctaata tcattaggtg gtcatgcagg acttggcgtt
     1201 gcgtttgcta aaaagaaagg tgaaatgtta tctgatattt gtatttatgc tacagcatgt
     1261 ggacatgcag gaattggtgg tgggataaat gcggctatca catattctga gaccaagtct
     1321 ttacctacat cgggagtcag taattcagta ggtgtaacgg ttggcggcgg agttgggggg
     1381 cattttgcgt atacttatgt agtggatgtt gataatccag aatcatcgac agaatctgtt
     1441 ggtatcggtg caggtgttga cgcttcagtt atgactctgg cttgtagaac gtggcaagaa
     1501 tgctgggtca attaacagga ggaaatatga aaaaatatat atttttagtt ttatttttat
     1561 tgtgtggttg ttctccagtg gacatagagc cagctagaca agaagttaat at
//
LOCUS       DAZKGF010000379         1584 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZKGF010000379 DAZKGF010000000
VERSION     DAZKGF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.381"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..613)
                     /gene="tssL"
                     /locus_tag="ACHOQV_005194"
     CDS             complement(<1..613)
                     /gene="tssL"
                     /locus_tag="ACHOQV_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFP9633400.1"
                     /translation="MRQDIDIDQLMMETWLTVTMLKKGALTPDGTALYNKCVKQVESV
                     REALERAGYDDASVEHITYAQCALLDETVMSRKPEKSGEGGEPDVDEGQAAWRKVPLQ
                     ARFFGSLRAGEALWDRIAEVLRQPSPDPAVLTCFHRVTALGFQGVYGLKAVSQSQRDE
                     VMKALSERVSLPDGGLSLVVYRMEKHRWRLIRSVWFWTVLAVIL"
     gene            complement(610..>1584)
                     /gene="tssK"
                     /locus_tag="ACHOQV_005195"
     CDS             complement(610..>1584)
                     /gene="tssK"
                     /locus_tag="ACHOQV_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP9633401.1"
                     /translation="VRYREEWVTLQDAFGSEEEPIAVARFNFTIRFAHENNDSWKVCP
                     VARLIRDGQNAWRQDPSFIPPVALFGASPVLRERLVLLNRQLRSRRQRLMAMRRESNE
                     RLADFAVADVSLFWLLNALNSHASVLTEYERFPSRPPEQVWAELARLAGSMLTFSLDH
                     DPDAIPGYDHKDPAGAFLPLFDLITGLLEACLPSRVIALEMSRPDGQTWKASLHDIRL
                     REEADLWLSVRSDTPAWQVAEKFPALCLAGSPDNVSEIYGAALRGIPLIPVSRVPAAL
                     PVRMENQYFALDMESPAAHEMLEQGVCMFYVPELLGALELELFAVLRS"
BASE COUNT          399 a          474 c          400 g          311 t
ORIGIN      
        1 taagaatgac ggcgagcact gtccagaacc agactgagcg tatcagacgc cagcgatgtt
       61 tctccatccg gtaaacaacc agggacagac caccatcagg cagagaaaca cgttcagaaa
      121 gggctttcat gacctcatca cgctgtgact ggctgactgc tttcaggccg tacaccccct
      181 gaaaacccag tgctgtcacc cggtgaaagc aggttaaaac ggccgggtcc ggtgaaggct
      241 gacgcagaac ctctgcaatg cgatcccaca gtgcttcacc agcccgaaga gaaccaaaaa
      301 agcgggcctg aagcggcact ttacgccagg ccgcctggcc ttcgtcaacg tccggctccc
      361 cgccttcccc gcttttttca ggcttacggc tcatcaccgt ttcgtccagg agggcacatt
      421 gtgcataggt aatatgctcc acactggcat cgtcataacc ggcacgctcc agcgcttccc
      481 gcacactttc gacctgttta acgcatttgt tgtaaagcgc agtgccatcg ggcgtgagtg
      541 cacctttttt cagcatggtg acggtaagcc atgtctccat catcagctgg tcgatatcaa
      601 tatcctgtct catgagcgca ataccgcaaa cagttcaagt tccagggcac caagtaactc
      661 aggcacatag aacatacaca ctccctgctc cagcatttca tgtgcagcgg gactttccat
      721 atcaagtgcg aagtactggt tttccatacg tacaggcaac gcagccggaa ccctgctcac
      781 cgggatcaaa gggatccccc tgagtgccgc gccatagatc tcactgacat tatcaggtga
      841 acccgccaga cagagtgccg gaaatttctc agcaacctgc caggccggag tatccgagcg
      901 cacagacagc cagaggtccg cctcttcgcg caggcggata tcgtgaaggc tggccttcca
      961 ggtctgtcca tccgggcggg acatttccag agcaatgacc cgggatggca gacaggcctc
     1021 cagcagaccc gttatcaggt cgaacagcgg aagaaacgca ccggccggat ccttgtggtc
     1081 ataacccggt atggcgtcag gatcatgatc cagggaaaaa gtcagcatac tgcctgccag
     1141 acgagcgagc tccgcccaca cctgttcagg tggacgggaa ggaaaacgtt cgtattccgt
     1201 cagtacactg gcgtgggagt tcagcgcatt cagcaaccag aaaagggaaa catcagcgac
     1261 agcaaaatcg gcgagtcttt cgttactttc acgacgcatc gccatcagac gctggcgtcg
     1321 ggagcgtaac tgacgattga gcaacaccag acgttcgcgc agaaccggac ttgccccaaa
     1381 taacgccacc ggaggaataa aagacggatc ctgtcgccac gcattctgac catctctgat
     1441 caatcttgct accggacaga ctttccatga atcattgttt tcatgggcaa accggatggt
     1501 gaaattaaac cgggcaacgg ctatgggctc ttcttcagag ccaaaagcat cctgcagcgt
     1561 gacccactct tcccgatagc gaac
//
LOCUS       DAZKGF010000380         1574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZKGF010000380 DAZKGF010000000
VERSION     DAZKGF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.382"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..399
                     /locus_tag="ACHOQV_005196"
     CDS             151..399
                     /locus_tag="ACHOQV_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9633402.1"
                     /translation="MSTQTGHFYTPSRILRMAELTRLLGISRSTIYEKMNPASKYYDA
                     TFPRPVRLGSSAVGWRASAIDEWLTLHTTPTRSVTKDK"
     gene            415..>1574
                     /locus_tag="ACHOQV_005197"
     CDS             415..>1574
                     /locus_tag="ACHOQV_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFP9633403.1"
                     /translation="MTKLSPPELDITKLPDRCQALLDEMHEETGISREILLSIMLTVK
                     AASVQDTHEVELSGGQRTSLQIYMCLSSASGSGKTSACTKLIAPVHETEEELHQAYID
                     DKKNYDRMMEMWTTDKKILERRYKKEMERSPENAAAARAALEKCIANKPVPPVQQVLI
                     VNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVTVERASRP
                     GFRVKDSRLTMLILTQPVIFDKFFTLTGDQIRGNGFLARVLFCEPGANKIMTTEPHTA
                     TPVVTQQCATCFHEKSFGSQIRDSLRASRERRAKGEQRICMTLSNAASHDLEIFHKEN
                     MSAVRQNPRMTTFEDIIVRKREQAVRIAALLELENNPHGTVITRESINSAIYL"
BASE COUNT          491 a          373 c          343 g          367 t
ORIGIN      
        1 aactcaaaaa ctgtacaaat atcatacaaa agatttgtaa acatgcgcat ctgctctgat
       61 ctccggttgg tttccgtttt atcacagggg acattctggc cttaacctag caaattaatg
      121 acatagcctt caactcacat ggagaacaat atgtcaaccc agaccggaca tttttatacc
      181 ccctcacgca tcctgcgtat ggctgaactt acccggctcc ttggtatatc aagatcgacc
      241 atctacgaaa aaatgaatcc ggcatcaaaa tactacgatg ccacctttcc ccggcctgtc
      301 cggcttggga gttcggctgt aggctggcgg gcatctgcca ttgatgagtg gttaacttta
      361 catacaacac caaccagatc cgtaactaag gataaataag gaaaactgta cgccatgaca
      421 aaattatccc cccctgaact tgacataact aaactgcctg acaggtgcca ggcattactt
      481 gatgaaatgc atgaagaaac gggaataagc cgtgaaatac tgctgtctat tatgctgacg
      541 gtcaaggctg cctcggtcca ggatacgcat gaagttgaac tttctggagg gcagcgtacc
      601 agtcttcaga tatatatgtg tctttcatca gcatcaggca gtggaaaaac ctctgcctgc
      661 acaaaattaa tcgcccctgt tcatgaaaca gaagaagaac tacatcaggc atatatcgac
      721 gataaaaaaa actatgatcg catgatggaa atgtggacaa cagataaaaa aatcctggaa
      781 cggagatata aaaaagaaat ggaaagatcc ccggaaaatg ctgctgctgc acgagcagcg
      841 cttgaaaagt gtatagcaaa taaacccgta cctcccgtac aacaggttct catcgtgaat
      901 gacgcgacac cagaagggat agctttgaaa ctcagccagt caccttctct tctgttgctg
      961 tctgatgaag gaggaactat tctggataag cgtttcgaac gcaaatcggc gctgtacaac
     1021 acattatgga gtgggcaacc agtcaccgta gaacgggcat ccagacccgg gttccgggta
     1081 aaggattccc gtctcactat gcttatactc actcaaccgg taatatttga taagtttttt
     1141 actctcactg gcgaccagat ccggggcaat ggctttctgg cccgggtgct attttgcgaa
     1201 cccggagcaa ataaaataat gacgacagaa ccgcatactg ccactcctgt tgtaacgcag
     1261 caatgtgcaa cctgttttca tgagaaaagt tttggcagtc agatcagaga ctctctgaga
     1321 gcgtcccggg aaaggcgtgc aaaaggtgag caacgcatct gcatgacatt atcaaacgca
     1381 gcatcccacg atctggaaat ctttcacaaa gaaaatatga gtgcagtcag gcagaaccca
     1441 cgtatgacca ctttcgaaga cattatcgtc aggaaaagag aacaggctgt tcgaatagcc
     1501 gcacttctgg agctggagaa caatccacat ggtacagtaa taacacgaga aagtatcaat
     1561 agtgctattt atct
//
LOCUS       DAZKGF010000381         1552 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZKGF010000381 DAZKGF010000000
VERSION     DAZKGF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.383"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(33..1451)
                     /gene="araE"
                     /locus_tag="ACHOQV_005198"
     CDS             complement(33..1451)
                     /gene="araE"
                     /locus_tag="ACHOQV_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFP9633404.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT          439 a          440 c          352 g          321 t
ORIGIN      
        1 ggccggatgt acagcacatc cggcccgtga aatcagacgc cgatatttct caacttttcg
       61 cctgccatca gtttgcgctc gatatgttcc agcgtgacat ttttggtttc cggaatgagc
      121 cagaaagtga tgcccacaaa cgcaatgttc agcgcagtgt agagccagaa cgtaccggca
      181 gcgccaatgc tatcaagcag tgtcaggaag gtcgcgccga taatcatatt cgacacccag
      241 tttgtcgtcg ttgagcaggt gatgccaaaa tcgcggcatt tcagcggctg aatttcagag
      301 cacaagatcc acaccactgg cgcggcgctc atcgcataac cggcaataca catcatcgtc
      361 atgccaacag agagccagga caagccactg gaagccgtac cgttatcaaa ctgcatcagg
      421 cagtaaccca gcaccagagt gcctaacgcc atcacgctga aaccaatttt cagagccggc
      481 ttacgccctg ctttatctac cgtaaagacc gcaatgaagg tagcgaacat aaaggtcagc
      541 cccacgacca gagtcgcaat catctgttgt tctgtggtcg taaagcccgc cattttgaag
      601 atacgtggcg cgtagtacat gatgatgttc ataccggtaa actgctgcat cgcctgcaac
      661 aacataccga gaaacacagc acgacggacg ttacggttga tcttaaacag tgcccaaccg
      721 ccctgtttta acttcaggct ttcacgaatt tcgttgagtt cttctcgcgc tttttccgac
      781 gtatcgcgca gcatacgcaa cacttcttcc gcctcaatat gacgcccttt ttccgccagc
      841 cagcgcgggc tatttggcag gaagaccacc agaataatca gcaggactgc tggtaaagca
      901 agaaccccca acattgcgcg ccagttaccg ctataactga acgctgtatc ggataaaaac
      961 gccagcacga tgccgagtgt gaccatcaac tggtacatac tgatcatctt accgcgaacg
     1021 ttttcacttg ccatttcaga aagatacaga ggagcggtgt aagacgcgat cccgacagca
     1081 atgcccagca ccacacgagc ggcgattaac atctctacgc tggtcgcaaa tgcggaccct
     1141 atcgaaccga gcacaaaaag gatagccccc gccatcaggc tgtatttacg ccccaggcgg
     1201 aacgacagcc acccattaaa cagcgcacca attgctgcgc cgagcatcat gctactgacc
     1261 acccattcct gcaaacggct ggtcagcaca aagtgatcgg taatgaacgg caacgctccg
     1321 gcgattacgc cgatatcaag accaaataac aatcctgcga ccgcagcagc taccgaaaca
     1381 aacatattca tacgccgcgt atcccgcaaa gaacgtggcg ttaaagcaga ttccgtattg
     1441 atagtaacca ttttttcctg ccagcagaga gtaagacata gtgaaaaaat acgtgaacaa
     1501 ttcacacagg tgtcaggtcg gaaatagcat aaatatggat taaattgctg cg
//
LOCUS       DAZKGF010000382         1547 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZKGF010000382 DAZKGF010000000
VERSION     DAZKGF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.384"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..216
                     /gene="cbtA"
                     /locus_tag="ACHOQV_005199"
     CDS             <1..216
                     /gene="cbtA"
                     /locus_tag="ACHOQV_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFP9633405.1"
                     /translation="AGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRAR
                     RATGLMTRDNYITVNNITLGKHPEAKR"
     gene            213..701
                     /locus_tag="ACHOQV_005200"
     CDS             213..701
                     /locus_tag="ACHOQV_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9633406.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLAELINMVCDNGHS
                     LRVVDESDRSTTDSMPPSAALSGIRCSTAHITETDNAWLYSLSHQTNDDGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            713..910
                     /locus_tag="ACHOQV_005201"
     CDS             713..910
                     /locus_tag="ACHOQV_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9633407.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            995..>1547
                     /locus_tag="ACHOQV_005202"
     CDS             995..>1547
                     /locus_tag="ACHOQV_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9633408.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGSKTAPDWAMKQDFRCGCWLMEKPDTAMKAITRNLDREIWRDLMQRS
                     GILSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINLFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWD"
BASE COUNT          406 a          400 c          386 g          355 t
ORIGIN      
        1 gcaggtattt cactgtgtga tgcggtgaac tttctcgttg aaaaatacgc cctggtacgt
       61 accgaccagc cgggattcag cgcctgtacc cgctctcagt taataaacag catcgatatc
      121 ctccgggctc gccgggcaac aggcctgatg acccgcgaca attacataac ggtaaataac
      181 attaccctgg ggaagcatcc ggaggcaaaa cgatgaaact ggccctgacg ctggaagctg
      241 acagcgttaa cgtacaggca ctgaacatgg ggcgaattgt cgttgacgtc gatggtgtta
      301 atctcgctga actgattaac atggtctgcg ataacggtca ctcccttcgt gttgttgatg
      361 aatctgaccg gagcacaact gacagcatgc caccctccgc tgccctgagc ggtatacgct
      421 gcagcaccgc acatatcacg gaaacagaca acgcctggct gtactcgctg tcacaccaga
      481 ctaatgacga cggcgaatca gaatggattc attttacagg cagcggctat ctgttacgta
      541 ccgatgcatg gtcgtacccg gttctgcggc ttaaacgcct ggggctgtca aaaacgttcc
      601 gccgtctggt tgtcacactt acccgacgtt atggcgtcag tctcattcat ctggatgcca
      661 gcgctgaatg cctgccgggt ttacccactt tcgactggta accagaaaca acatgaaatc
      721 attaaccacg gaaacagcgc tggatattct gattgcgtgg ctgcaggaca atatcgactg
      781 cggatcggga attatctttg acaacgatga ggataaaacg gattcagcag cactgttgcc
      841 ctgtatcgaa caggcccggg aggatgtccg taccctgcgt catctgcagc ttctgcacca
      901 gaaccggtga agctcactca ccagacttca ttcctctgac gccagcctgc acagggctgg
      961 cgttttcatt tatctgcaaa aaggaatatc gattatgtca gatatcacaa tctcccgtcc
     1021 ggaagtggtc aacgggcata cggacgttat ctgctcaacc agcattcgcc acattctggc
     1081 tgtacgaaag agtacactgt tgcaaatcga cacacttatc cgacaactgg ctgaaatctc
     1141 ggtgctgacg gaaagcatcg gaagtaaaac tgccccggac tgggccatga aacaggattt
     1201 tcgctgtggc tgctggctca tggagaaacc tgatacagca atgaaagcta tcacccggaa
     1261 tctcgatcgc gaaatctggc gtgacctgat gcaacgttcc gggattcttt ccttaatgga
     1321 tgcgcaagcc cgtgatacat ggtaccggtc actggagtac gataattttc cggaaatcag
     1381 cgaggcgaac attctgagca catttgaaca actacaccag aataaggatg aagtgtttga
     1441 gcgaggagtg atcaacctct tcagagggtt gagctggaat tacaaaacca attgcccctg
     1501 taaatttggc aataaaatta tcgtcaacaa tctggtgagg tgggacc
//
LOCUS       DAZKGF010000383         1529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZKGF010000383 DAZKGF010000000
VERSION     DAZKGF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.386"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..947)
                     /locus_tag="ACHOQV_005203"
     CDS             complement(<1..947)
                     /locus_tag="ACHOQV_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9633409.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVKIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSEVIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAK"
     gene            complement(973..>1529)
                     /gene="rfaY"
                     /locus_tag="ACHOQV_005204"
     CDS             complement(973..>1529)
                     /gene="rfaY"
                     /locus_tag="ACHOQV_005204"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFP9633410.1"
                     /translation="VSLIDTKYGRYVFKVFAPKTKRNERFFKSFVKGDYYQNLIVETD
                     RVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASM
                     EKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIK
                     DYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          475 a          319 c          266 g          469 t
ORIGIN      
        1 ttctttgcat gttggtattt cacttgatac tgcttttcat tcgtggctgg aataaacgcc
       61 acatcattcc agcaagaagc ctgataagcc tcattaaaat acttctggca gggataattc
      121 gtccagtcgt tccagggctt cgttacgcca acataatgaa tcagcttagt atcatcggta
      181 atggtctttt tataatcttc atcgctttta ttttttaact catagtcaat gccataaaag
      241 caatcataat cgccgctaat aaaaatacaa tgaccaaaga acagaatatt taaaatatcc
      301 tgatcaaggc atttatattt cgtgtgctgc gggtcacttg ccagcatagc aatggcttta
      361 ttcataaaat catttttcgc ccatgcttca agctgcagat atactacacc ggcgttgaaa
      421 taattacctg gaagtccctc tatagccaga cgcttcgctg gtttttcctg cataaaaggc
      481 acatctttaa taacagctgc gaacttatcg ccatcaaaat taatatcaag caatccagct
      541 aatgagcctt tacagatgac atcagcatcg atatataaca gattttcaat aacttcactc
      601 agatactgga acgcaactaa acggaagaat gttgcataag accactgttt caggctgggg
      661 aagatatcaa taaaggcact ggagacttta taaattttta cttcaacgtt agcatttttg
      721 ctggtaaaag cgttaacacg ttgtagataa tcttcatcaa tgtagtcggt aaagagatga
      781 aacttaaccg caaggtccgg gttatgcata acaactgatt gcatagatac tgccgcacca
      841 aataaaaaat tacggtcaat accgtaagcg atatttaaca cacgatcggt gttagcaatt
      901 tccccagaga cagcgacttc agtaatactc tcagctaata aatccatgtt ggttccgttt
      961 tgactgtgtg gtttacgctt tgccttttaa ttttttaata aatttgcgta gtttggttct
     1021 gtagattact gaatagtagc cataatcttt tatttcgttg gcaataccca ggtgacgttc
     1081 catggccaga cgatcacgag ctttacgctc tgccgtacag cttttaccgg ataggtcaat
     1141 tatcctgaca ccatctttac taacgataaa gttaccccgg tgagggtcgc ccgatagcat
     1201 atttaaggca tgcaattttt ccattgaagc cttaatttct gctttaacat tttctggaat
     1261 aatcggcata tcattgagtt ctacgccctc gacatactcg ataagcatga taaaaacact
     1321 ggcatagttg aaaattttac gctcagccag aaaataaaaa tcattaggaa aagtaagacc
     1381 tgcacttctg acacgatcgg tttcaacgat caaattctga tagtagtcac cttttacaaa
     1441 agatttaaaa aaacgttcat tccttttcgt tttgggagca aagactttaa aaacatagcg
     1501 accatatttc gtatcaatca acgatactt
//
LOCUS       DAZKGF010000384         1514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZKGF010000384 DAZKGF010000000
VERSION     DAZKGF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.387"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..999)
                     /locus_tag="ACHOQV_005205"
     CDS             complement(1..999)
                     /locus_tag="ACHOQV_005205"
                     /inference="COORDINATES: protein motif:HMM:NF016702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFP9633411.1"
                     /translation="MNALQRVAGGTAPYIAEIIKQTTPDGAGRVAAHAVVNAALSLAQ
                     GKNALAGAAGAATGKMVGMIATQMYGKPVSELSETEKQTVSTLATVAAGLAGGLVGDS
                     GASAVAGAQSGKTTVENNALSLPSGMVSYGQAVASWNQYADANNLTPEQKQAGLDKLA
                     KGELPEGANISKVIVDGYKDGVLIAGAWYLGPAVSVGKAAVGGVTSAAVNGGFQWYGL
                     SQPGSENQSWDYKGSASSFMTGMLAPGRTVTANTAIALGGSVFSDGANIKSQSGTALG
                     AYAGGYVGEYFPVILRPVLGNSASTVGDIGGGFVSELINYGIKNYPTPTNKEPEHE"
     gene            complement(1006..1278)
                     /locus_tag="ACHOQV_005206"
     CDS             complement(1006..1278)
                     /locus_tag="ACHOQV_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000906734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633412.1"
                     /translation="MLINKKHPDINLKLLLLSIVYLIACCCVFFLIASIVIDVVFNDK
                     VNVTREVIIDIIVVSTIVGIAGGVRAWIFAKIDERKTRKSQPSDPD"
BASE COUNT          395 a          414 c          278 g          427 t
ORIGIN      
        1 ttactcatgt tctggctcct tattagtagg agtaggataa tttttaatac cgtagtttat
       61 taactcagaa acaaagccac caccaatatc accaacagta cttgcagagt tacctaacac
      121 tggcctcaat atcacaggga aatattctcc cacatatcca cctgcgtatg ccccgagagc
      181 agttccagat tgggacttaa tattagcacc atcgctaaat acagatcccc ccagcgctat
      241 agctgtatta gctgtaactg tacgacctgg agcaagcatc cctgtcatga atgaagatgc
      301 gctaccttta taatcccatg actgattttc actaccaggt tggctcaatc cataccattg
      361 aaaaccaccg ttcacagcag cggaagtaac tccaccgacc gcagctttac ccactgacac
      421 cgcaggcccc agataccagg cacctgcaat caatacgcca tctttataac catcaactat
      481 aactttgctt atattggctc cttcaggtaa ctcacctttg gcaagtttat ccagtcctgc
      541 ctgcttctgt tccggggtga gattattggc atcagcatac tgattccagg aggcaaccgc
      601 ctgaccataa ctcaccattc ctgatggcag actgagcgca ttattctcaa cagtcgtctt
      661 acctgactgt gcgccggcta ccgctgaagc gcctgagtct cccaccagac caccggccag
      721 gccggcagca accgttgcca gtgtcgaaac agtctgcttc tctgtctcgc tcagttcact
      781 caccggttta ccgtacatct gtgtggctat cattcccacc atcttgccgg tggctgcacc
      841 tgcggcaccg gcaagggcgt tcttaccctg tgccagagac agggctgcat tcactactgc
      901 atgagctgct acacgccctg ctccgtccgg ggtggtctgt ttgataattt cagcaatata
      961 cggcgcggta ccacctgcca ctctctgtaa agcattcata aaggtttaat ctggatcaga
     1021 aggttgcgat ttacgagtct ttcgttcatc tatcttggca aagatccatg ctctcacgcc
     1081 cccagcaata ccaactatcg tacttacaac tatgatatct ataataactt ctcgtgttac
     1141 atttacctta tcattaaaaa ccacgtcaat aacgatactg gcgataagaa aaaacacgca
     1201 acaacaagca ataagataaa caatacttaa taataaaagt ttcaagttta tgtcaggatg
     1261 ttttttattt attaacatta ctttctccta tactattcct gacgttattt ctctgtcgag
     1321 caggaagttt ttcgccggtt gcttccttgg tggccttcaa cgcattcagt tccccctgtg
     1381 tacgggcaat atcttcactt attaaaactt tatcaaaacc aacgatattt cagtgggatg
     1441 tgtgcaagtt gttcatcaac cgaccagttt tgtggttccg attttttatt catccatttc
     1501 gcaccactgt ctaa
//
LOCUS       DAZKGF010000385         1510 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZKGF010000385 DAZKGF010000000
VERSION     DAZKGF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.388"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            266..841
                     /locus_tag="ACHOQV_005207"
                     /pseudo
     CDS             266..841
                     /locus_tag="ACHOQV_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
     gene            839..>1510
                     /locus_tag="ACHOQV_005208"
     CDS             839..>1510
                     /locus_tag="ACHOQV_005208"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP9633413.1"
                     /translation="MEIVPGEQPGESDILITRKQDKYWRLGFSFDDSGTRSTGRYLGG
                     ITFSLDNPFSLSDLLYVSATHNFPHYGGKGSKNYTAHYSVPFGYWQFSVTASDYDYHQ
                     TVAGAVEDYQYSGESQNLGMQLSRVLHRNGTQKTVLTYDVQARRSRNYINDTEIQVQR
                     RQTAAWRLGLQHRHYISQAILDAGVSWQRGTRWFGAQPAPEEIFGEATALSKILQLNA
                     SFNLPF"
BASE COUNT          356 a          353 c          412 g          389 t
ORIGIN      
        1 ttctataatg atcaaaatta agtcagctaa aaattggcag atttattatt cagcattctg
       61 gtaccggttg atgcgggtaa ataccggaag ggcggtagcg tgccatcgca aaacatggtg
      121 ggaggtatcg tcaggaggct ggtgattgtt ggtataacat gaataaaagg tttttcgttt
      181 gactggaaaa ttatgttgca ggaacgagag ggctgaggaa caaagcttat tccggcgctg
      241 tccgtccttg ctgtcactga cactgatgtc tgttttatta tcgcctttat ctcttcaggc
      301 cgcggatatc cggcgtagtg gagaggatac atttatcatt cagcagcagc gtcaggaagc
      361 ccttgagcaa caactgacgc cttcagcccc tgatgttcgt ctttctgcac ctggctcttt
      421 tgcccataag attaattttc ctgttgaaac gccctgtttt cagattaaac agacggaact
      481 gaagggggct gatgcgttac cacactggct gcctttacaa aaaatcgcca acggggcggt
      541 cgggcattgc ctgggggcga aaggaattaa tctgctgatg agtacattgc agaaccgtct
      601 ggttgatcat ggttatgtca ccacccgggt tctggcacct tcgcaggatt taaaaagcgg
      661 tatcctccgg ctggttatta ttcccggtgt tgtacgacat gtgcgtctga caccgggcag
      721 tgatgactat attcagttgt attcctcatt tccggcacac gaaggctctc tgctggattt
      781 acgggacatt gaacaggggc tggagaatat gcaacgactg ccgggtgtgc aggctgatat
      841 ggaaattgta cccggtgagc agccagggga aagtgacatt cttattaccc gcaaacagga
      901 caaatactgg cgactggggt tttcgtttga cgattcaggt acccgcagca cagggcgtta
      961 tctcggtggt ataacatttt ctctggataa tcctttttcc ctgagcgacc tgctgtacgt
     1021 ttctgccacc cataattttc cacattacgg gggtaagggc agtaaaaact acacggccca
     1081 ttactcagtg cctttcggct actggcagtt ttccgtcacc gccagtgatt acgattacca
     1141 tcagacggta gctggtgcgg ttgaggatta ccagtacagt ggcgagagtc agaatctggg
     1201 catgcagctg agccgggtac tgcaccgtaa cgggacgcag aaaacggtgc tgacttacga
     1261 tgttcaggcc cgcaggtcgc gtaactacat taatgatacg gaaatccagg tccagcgccg
     1321 gcagacggcg gcatggcgac ttggactgca gcaccgtcat tatatttcgc aggccatact
     1381 ggatgccggc gtcagctggc aacggggaac ccgctggttt ggtgcacagc cggcaccgga
     1441 ggaaatcttc ggtgaggcaa cggcgctcag taagatcctg cagttgaatg catcctttaa
     1501 tctgcccttt
//
LOCUS       DAZKGF010000386         1504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZKGF010000386 DAZKGF010000000
VERSION     DAZKGF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.389"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(41..>1504)
                     /locus_tag="ACHOQV_005209"
     CDS             complement(41..>1504)
                     /locus_tag="ACHOQV_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFP9633414.1"
                     /translation="RNASLTAIHVNISDNNLPTECITVDYDYILCKHLIVNTEFDWLA
                     FPAGYNSLFIPPKSPPASNLKPEPLPVVNLPLSSPAVNPVYAKSCLKEKGCTDAGTAE
                     EPAENFGQVAIFAQPVVDDCCGYHHPEANDVGQPAEAQTMLLFPGSVLAAQIWGKWSL
                     SGILSATRGSYIGALASALYIPSAGEGSARVPGRDEFWYEEELRQKALAGSTATTRVR
                     FFWGTDIHGKPQVYGVHTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVDGPLITWTP
                     ENPEHGNVPGHTGNDRPPLDQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPA
                     GSGIKPIYVYLKEDLRKLPGVVTGRGVPLSLGTRWLDMSVSNNGNGAPIPAHIADKLR
                     GREFKTFDEFREALWLEVSKDSVLLAQFIKSNQNNVSQGYSPYVPEEGYYYGPNEIVK
                     KFQIHHVVAIEHGGGVYDIDNFRIVTPRLHDEIHYRR"
BASE COUNT          385 a          380 c          351 g          388 t
ORIGIN      
        1 gtgtaaaaat atattttatc ttttaaatca cgcatataca ttatctcctg tagtgaattt
       61 catcatgtag tcggggcgta acaatcctaa aattatcaat atcatacact ccaccaccat
      121 gttctatagc aactacatga tgtatctgga atttcttaac tatctcattt ggtccgtaat
      181 aatacccctc ctcagggaca tagggggaat atccttgact aacgttattc tgattacttt
      241 ttataaactg cgccaataac actgagtctt tactcacctc cagccacagc gcctcgcgaa
      301 actcatcaaa ggttttaaac tcccgtccgc gcaatttatc agcaatatgc gccgggattg
      361 gtgcgccgtt gccgttattg gataccgaca tatccagcca gcgagttcct agtgaaagcg
      421 ggacgccgcg ccctgttaca acaccaggca gctttcgcag atcctccttc aggtaaacat
      481 agatgggctt aataccggat cccgccggaa aaaccagaat ataatcgttg aattctttcg
      541 gatcaggaac cgggaatggc ggcgtggtat aggtatcggt gccgtccgga atcggcgtca
      601 ccagaatggt gggctgatcc agcggcggcc tgtcgttacc ggtatggccc ggaacattcc
      661 catgttccgg attttccggc gtccaggtaa tcagggggcc atcgacatcg tgagcggggg
      721 taaattcata acgctgcgtc tgctcgttcc actgcatgtt cgccacgcgg acgttttcat
      781 acggcgtacc ttcacccgta tgaacaccat acacctgagg cttgccgtga atgtcagttc
      841 cccagaaaaa acgtacccgg gtggtggcgg tactgcctgc tagtgctttc tgccgcagtt
      901 cttcctcata ccagaactca tcacgtccgg gcacgcgagc actgccctcg cccgcagagg
      961 gaatatacaa agcagatgcc aacgcaccga tgtaagagcc gcgggttgca ctgagtatgc
     1021 cactgagcga ccattttccc catatttgag ccgccaacac actacccgga aacagtagca
     1081 tggtttgagc ttctgcgggt tgcccgacat cgttagcctc gggatggtgg tatccacagc
     1141 agtcatcaac cactggctga gcaaaaatcg ctacttgacc gaagttttca gcgggttctt
     1201 ctgccgttcc agcatctgta catcccttct ccttcagaca ggattttgca tagaccgggt
     1261 taaccgctgg tgaagagagt ggaaggttaa caactggtag aggttcaggc ttaagattac
     1321 tggcaggtgg gcttttaggc ggaataaata agctattata gccagcagga aaggccaacc
     1381 agtcaaattc cgtattgact attagatgtt tacataatat gtagtcataa tcgacagtta
     1441 tacattcggt aggaagatta ttgtcagaaa tgtttacatg aatagcagtt aatgaagcat
     1501 ttct
//
LOCUS       DAZKGF010000387         1488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZKGF010000387 DAZKGF010000000
VERSION     DAZKGF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.390"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            49..1455
                     /gene="gndA"
                     /locus_tag="ACHOQV_005210"
     CDS             49..1455
                     /gene="gndA"
                     /locus_tag="ACHOQV_005210"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP9633415.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIV
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          378 a          358 c          380 g          372 t
ORIGIN      
        1 agttattctc ataccgcaca tgcggtaaaa ccctgacagg agtaaacaat gtcaaagcaa
       61 cagatcggcg tcgtcggtat ggcagtgatg gggcgcaacc tcgcgctcaa catcgaaagt
      121 cgtggttata ccgtctctat tttcaaccgt tcccgtgaaa agacggaaga agtgattgtc
      181 gaaaatccag gcaagaaact ggttccttac tatacggtga aagagtttgt tgaatctctg
      241 gaaacgcctc gtcgcatcct gttaatggtg aaagcaggtg caggcacgga tgctgctatt
      301 gattccctca agccatacct cgataaaggt gacatcatca ttgatggtgg taataccttc
      361 ttccaggaca ccattcgtcg taaccgtgag ctttctgccg aaggctttaa ttttatcggt
      421 accggtgttt ccggtggtga agaaggtgcg ctgaaaggtc cttccattat gcctggtggg
      481 cagaaagaag cctatgaact ggttgcaccg atcctgacca aaatcgccgc agtggctgaa
      541 gacggtgagc catgcgttac ctatattggt gccgatggtg caggtcacta tgtgaagatg
      601 gttcacaacg gtattgaata cggcgatatg cagctgattg ctgaagccta ttctctgctt
      661 aaaggtggcc tgaatctcac caacgaagaa ctggcgcaga cgtttaccga gtggaataac
      721 ggtgaactaa gcagctacct gatcgacatc accaaagata tcttcaccaa aaaagatgaa
      781 gacggtaact acctggttga tgtgattctg gatgaagcgg ctaacaaagg taccggtaaa
      841 tggaccagcc agagtgcgct ggatctcggc gaaccgctgt cgctgattac cgagtctgtg
      901 tttgcacgtt atatctcttc tctgaaagat cagcgcgttg ccgcatctaa agttctctct
      961 ggtccgcaag ctcagtcagc aggcgacaag gctgagttca tcgaaaaagt tcgtcgtgcg
     1021 ctgtatctgg gcaaaatcgt ttcttacgct cagggcttct ctcagctacg cgcagcgtct
     1081 gaagagtaca actgggatct gaactatggc gaaatcgcga agattttccg tgctggctgc
     1141 attatccgtg cgcagttcct gcagaaaatc accgatgctt atgccgaaaa tccgcagatc
     1201 gctaacctgc tgctggctcc ttacttcaag caaattgccg atgactacca gcaggcgctg
     1261 cgcgatgtcg ttgcttatgc agtacagaac ggtatcccgg ttccgacctt cgccgctgcg
     1321 gttgcctatt atgacagcta ccgtgccgct gttctgcctg cgaacctgat ccaggcacag
     1381 cgtgactatt tcggtgcgca tacttataag cgcattgata aagaaggtgt gttccatacc
     1441 gaatggctgg attaatctga tttaatcaac aataaaattg aggcccgg
//
LOCUS       DAZKGF010000388         1488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZKGF010000388 DAZKGF010000000
VERSION     DAZKGF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.391"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..353)
                     /locus_tag="ACHOQV_005211"
     CDS             complement(<1..353)
                     /locus_tag="ACHOQV_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9633416.1"
                     /translation="MTTFSHSSTTPSVSVTTASGSDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIA"
     gene            complement(444..1262)
                     /locus_tag="ACHOQV_005212"
     CDS             complement(444..1262)
                     /locus_tag="ACHOQV_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9633417.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT          338 a          388 c          419 g          343 t
ORIGIN      
        1 gcaatgccgg cggcttccgg gctcatttca gcgcggttac cgttcatggc attgaacagt
       61 acccagtttt cgccatcatc gtcatccggt tcgggggcca taaatgcgcc accgttgttc
      121 agtgtgtaca gattccagat accaccgcag tagtcttcgc aaagacggtc catccagccg
      181 aagatacggg gctccagggt cacccactgt ggaatgaggc caaaatgctg cggccagaag
      241 ctgatgcgct gttcatcagg gactatggtg gcaaccagct gaggctggtc actccctgat
      301 gcagttgtta cggaaacaga aggtgtggtg gagctatgcg aaaaggttgt catgaaatga
      361 ttccttataa aaagcaaatg aatgaagaag actcgggggc agggacagac gtgagtcagg
      421 acggcgcttt caggtaaacg gtatcagcgc atgctctcca gcagcgtttc cgccatcacc
      481 cacaatgcgc gattgagctt gatgtcggtg tcgatactgt gaatggcacg ggtatggata
      541 cgttttcctt ttgcactacg gccggaaatc ccgcctttca gcatattctc ctggatggtc
      601 tgatatgcgc tccacaggtc cttaccgtaa tcctcccggc gtcgtggtgt cagaatgtcg
      661 gcagtggtga cgggttgatg ttcatcacca taacggtaag tcagcgccgc ctgtgccagt
      721 gcctgacgtg ccggtggcgg cagaaccaga gactgcatgg catcacgctt ttcctcaatc
      781 cggtcaaaaa cgcccactac ctcgtaagcc ccttcaatga ctctgtccac cacatttccc
      841 cggtggggca cacggacttc ccccagagac tgaccgcaga cgcagccgtt ctggcagacg
      901 aatctgaagt aacccggcag catctggtag ctggaggtac cgtcatggga gttgagcaga
      961 ataatttcag gcacatgctg accggttatc tgtccggccc gccgcagacg cagcatgtgt
     1021 ttggtatatt cccggcggcc cgggtcacgt acgcgggtct ggcaggcgaa gaatggctga
     1081 aagccttccc gctgcaggct ttccagcacg gtgatggtgg gaatccaggt atagttctcg
     1141 cttctggatg tgtgtttctc ttccccgaat acgctgggga cgacctgcat cagttcttca
     1201 cgagttaagg ggcggtcacg gcgtatctgg ttcgcataac caaaacgact ggctaatcgc
     1261 atgatttatt ccttatgaat gagttagcgg tataaatgaa aaagccgcgt ctcccggaga
     1321 agacgcggcc tgatggggga aatgaatgac gtttattgtc tgagaagccc ttcactggcg
     1381 agctgcgtat ttttatggaa ggagatcacc caataacccc atcgggatcg ggtaataacc
     1441 ggtcagactg tgatcgattc agatgccatt tggcagcaaa agagcctt
//
LOCUS       DAZKGF010000389         1472 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZKGF010000389 DAZKGF010000000
VERSION     DAZKGF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.392"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(55..288)
                     /locus_tag="ACHOQV_005213"
     CDS             complement(55..288)
                     /locus_tag="ACHOQV_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633418.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALNAAYSEHPEWFSGLVSGDEN"
     gene            complement(384..1007)
                     /locus_tag="ACHOQV_005214"
     CDS             complement(384..1007)
                     /locus_tag="ACHOQV_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9633419.1"
                     /translation="MRTRIDYLANKYSFTELNESPRLRWQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLIRTPQLIAALREEWDISQKNVMFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNESTSSPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRQSGMPVVTTSVEVSDNLTGTTRKIPAYHL"
     gene            complement(1096..>1472)
                     /locus_tag="ACHOQV_005215"
     CDS             complement(1096..>1472)
                     /locus_tag="ACHOQV_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633420.1"
                     /translation="IHAFCTSFYRQVHERITERNHTASPSSPMVLRWLREQHRGATIR
                     CLLLLSQTSICHPRASVTVDEECSQLVDLLQQTWKVISAGGQCRVEKRFQVVRGDTSG
                     QYVALKTVVQSFVLPVMTTINW"
BASE COUNT          370 a          406 c          333 g          363 t
ORIGIN      
        1 aagaaaaaat ccctgccggc aatcacactg cctgatggca gcataatcct ctttttaatt
       61 ctcatccccg gagacaagcc ctgaaaacca ctcgggatgt tcactgtatg ctgcgttcag
      121 agcatcccag gaaacgttca tacagaagct gtcctgcaat ttgcaggcaa ccacctctgc
      181 caggcggttc cggttatccg ccagtccggc ataacctctc tctgccagcc actcattcag
      241 ttcatgttcc ggagacagat tgataatatc gtaatcggac cttgctattc ccgtgtcctt
      301 agcacatgtt ttcaggcacg gggataaagt ataaagaatg tcgtactgct gctgccagag
      361 caatattccc cctgatggtc gtatcagaga tggtatgccg gtattttgcg ggttgttccc
      421 gtcaggttat cgctcacttc cacggacgtt gtcaccaccg gcattccgct ctgtcttagt
      481 ctcaggacat cagccgcaat acgctgactg ccagaccaga aaaggccgtc cagtgcggtc
      541 accagtaagc ccgcctccag cgcatacttc agccgttcac ggggcgcttt cacttccctg
      601 gcaacctgct gatatggcga tgatgtgctt tcatttccaa ccacgcgacg aatacggtga
      661 gacagatgat accggtatgt atccggcaca ccggaaaggc tggccttcag gctgtacacg
      721 cagccaaagc gtttatcgtt gaacatcaca tttttctggc tgatgtccca ttcttcacgc
      781 agcgcggcaa tcagttgtgg tgtacggata agcaacaagc gaaaaggcag ttcaaaactg
      841 gtgacgtaat ccacattcag cagagcaata cgcagccgtt cttctggccc ggcctctgtc
      901 tgccggcact cctccagaac atcctgccac tgccagcgaa gacgggggga ttcattcagc
      961 tctgtaaaac tgtatttatt cgccagataa tcaattcgtg tacgcatact gaaaagaatt
     1021 ctgtataaaa attcatctga caaaacttta tcagtccata agaatcaacg gaagcgcagc
     1081 tggttcacac gaccatcacc agttgattgt tgtcataacc ggtaacacaa aggattgaac
     1141 gactgttttt aacgcaacat actggccaga tgtatcaccc cggacgacct gaaaacgctt
     1201 ttccacccgg cattgtccgc cagcgcttat caccttccag gtctgttgta gtaaatccac
     1261 cagttgtgaa cattcttcat caactgtgac actggctcgc gggtgacaaa tactcgtctg
     1321 gctgagcagc aacaggcatc gaattgttgc tcctctatgt tgttcccgca accagcgtaa
     1381 taccatcggc gaggatggac tggccgtgtg attacgctca gtaatacgtt cgtgcacctg
     1441 tcggtaaaag gaagtacaga atgcatgaat ct
//
LOCUS       DAZKGF010000390         1460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZKGF010000390 DAZKGF010000000
VERSION     DAZKGF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.393"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(56..1192)
                     /locus_tag="ACHOQV_005216"
     CDS             complement(56..1192)
                     /locus_tag="ACHOQV_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP9633421.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
BASE COUNT          438 a          331 c          247 g          444 t
ORIGIN      
        1 aataaaagcc tctgatataa ataaaaacta tgtaaatcgt ttaaatggat acctgttaaa
       61 actgatacat cataccaaca gcaagtatat tatcggttgc aactccagcc tttttggtaa
      121 attcgctttc atcaataagg ttaattttgt aatcaataag aagtgacatg tttttgttca
      181 gaaagtagtt tgcgccaaca tcaatatact caacaagaga ctgatcgcca tagtttacta
      241 ttccaccttt aacatccatc cctttggact gaacgtaagc cagtgaagga cgcaggccga
      301 aatcaaacat atactgagcc acaacttcaa tattccgtgc tttatctgcg atgcctgcgc
      361 taccaaatgg agtcatattt cgggtttcag agtagtcagc tgccagatac aggtcattat
      421 cattatactt gaccccaaca cctgcaaact ccgcatgttt tcccccggca atccagcgag
      481 gcgtaataac ttcttttcct tctgaatctt tcgatgtcat gaaacgttta cggccttctt
      541 tttcctgcag actggttctg tcggcattag tgtaagcggc aacgattcca aaaccttcat
      601 agtcatagct tacggagaaa ccatatccat caccattagc ttcaagtaaa ttatcacggt
      661 catttttacc ctgatattga gcagaaaaag taagtccatc aacaagcccg aagaaatctg
      721 tattgcgcca ggtcagaact ccggttgttc tgccggtcat atagttatca gagtaagtcc
      781 agctgtcacc accaaagtcc ggaagtacgt cagtcagagc ccccacatca taggcaacac
      841 catagtttcg cccataatca agtgaaccga gtatattgtg gcgaatacct gcaaatgcca
      901 gacgattttt atcgttgcgt acttcacttt ctgtcttaaa ccccaggaac tggtattccc
      961 actgaccaaa gccagtcagg ctctcattaa tttgtgtttc gcctttgaaa ccaagacgtg
     1021 caaatgttct gtcgccatcc atagaaccat cagagatgta atgacgggct ttaacgcgac
     1081 cataaaaatc cagtttatta ccgtctttat tatagatttc tgctgcctgt gttgcctcac
     1141 acagcattaa tgcagaaacg acagagcaca caaccatact tagttttcgc ataaaaaaat
     1201 cccctgtgca catttttagt atggttttaa aaattaaact atggcgaaaa aattatcact
     1261 atgcaaagat gtaacaaaat attgctcagt gatctatatt gttgttttta tttgaggttt
     1321 tatattttct agtgtatttg acttttcttt ctcaagtgtt atttcttgac ctttatcaca
     1381 gaaccatctc cctggatatc cccatcacat tgcatgaaaa gcaagtttta ccaatcttga
     1441 ttttcagtgt taaataagat
//
LOCUS       DAZKGF010000391         1446 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZKGF010000391 DAZKGF010000000
VERSION     DAZKGF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.394"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            62..796
                     /gene="nanR"
                     /locus_tag="ACHOQV_005217"
     CDS             62..796
                     /gene="nanR"
                     /locus_tag="ACHOQV_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP9633422.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(832..>1446)
                     /locus_tag="ACHOQV_005218"
     CDS             complement(832..>1446)
                     /locus_tag="ACHOQV_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9633423.1"
                     /translation="YTLFVLSYRLPSPAEEENINKTFIDAISAMLYIRKNADKWHLQR
                     HRIGVLGFSAGGHIASTFVNQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTH
                     PGSRKQLLGDNSTSENIIQYSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIE
                     KGVSAEMHLYEKGGHGFSVQWATDLPVGGWIKLFMDWMYSHGFK"
BASE COUNT          510 a          294 c          253 g          389 t
ORIGIN      
        1 cattattaaa agaaagtgtt aaattaaaaa ctcatttgct ctaatccata taaatcaata
       61 tatgtccgac aacaaaaaaa ccgtgttacc cgggctaaca acgaccagaa agcccattgt
      121 tacaaaaaaa ctttcagaaa tcgttgccga agagcttgaa agaatgatcc gccaggagga
      181 gataagggaa ggtgaatacc tgccttctga acgtgaactt atggcattct ttggcgttgg
      241 aagaccatct gtcagagagg ctttatcatc tctcagacat aaagggctaa tccggtataa
      301 caagggagaa aaagccagtg tctgccgtcc atcaccagaa accatcatta atgggttatc
      361 aggcattgtt aaagattttt tatccacagc tgatggcatc aaggattttg aaagactacg
      421 catctttttt gaaaccaacc ttgtaagata tgcagcaaaa aatgcaacgc ctgaggatat
      481 tcacaggctt gagaaggtgc tcagtatcca tcataactca ctgagaaccc ccgaagtgta
      541 tgctcaatat gatattgaat ttcatagtat acttgctgat atacctgaca ataaagtatt
      601 aaaaacaata cactcagcat tttctgaatg gctgataatg gccaaacctc tggaaaagaa
      661 taaaatgaca tatgaaaata acaaaaaaac ccatcagcag catattgaaa tacttacggc
      721 cataaaagag catgattgtg ataaagctga acagctaatg tataatcacc tttataaacg
      781 aattgaagca tcatagttac tcgacgcttc accaggtagt caccataaat attatttaaa
      841 tccatgagaa tacatccagt ccataaacag ttttatccat cctccaacag gaagatctgt
      901 ggcccactga acactaaagc catgccctcc tttctcatag agatgcattt cagcagaaac
      961 gcctttctct attaggccac ggtacatgag cagactgttt tcaattgaca caagatcatc
     1021 atcacaacaa tggagtaaaa acacaggagg aaaacatgat gtgacattaa gatccgcact
     1081 atattgtatt atattttctg aggtactatt gtcccccagt aattgcttcc tggagcctgg
     1141 atgtgtcaca tcatctttca ttgatattac aggataaatt aacccgacaa aatcgggaat
     1201 agcgaaaatc tcttgttctt tactgtcaga agaacgtttg aaatcacaaa tctgattcac
     1261 aaacgttgag gcgatatgcc ccccagcaga aaatcctaat acaccaatac gatgacgctg
     1321 tagatgccat ttatcagcat tcttacgaat ataaagcatt gcagatattg catcaataaa
     1381 agttttgttt atattttctt cctcagctgg agatggtaat ctgtaactaa gaacaaataa
     1441 tgtata
//
LOCUS       DAZKGF010000392         1438 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZKGF010000392 DAZKGF010000000
VERSION     DAZKGF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.395"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..471
                     /gene="curA"
                     /locus_tag="ACHOQV_005219"
     CDS             <1..471
                     /gene="curA"
                     /locus_tag="ACHOQV_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFP9633424.1"
                     /translation="ATEVLGFDVCLDHHADDFAEQLAKACPKGIDIYYENVGGKVFDA
                     VLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKRIRLQGFIIAQDYGHR
                     IQEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNFGKVVIRVAVDD"
     gene            669..1334
                     /gene="mcbR"
                     /locus_tag="ACHOQV_005220"
     CDS             669..1334
                     /gene="mcbR"
                     /locus_tag="ACHOQV_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFP9633425.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVTLAVE
                     NLTPQDLAELEELLEKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          425 a          302 c          348 g          363 t
ORIGIN      
        1 gctactgagg tgctgggctt cgatgtttgt cttgaccatc acgcggatga ttttgccgaa
       61 caactggcga aagcgtgccc aaaaggtatt gatatctatt atgaaaacgt gggcggtaag
      121 gtatttgatg cagtgctacc tttacttaat acatctgcgc gcattcccgt ttgtggctta
      181 gtgagcagct ataacgctac agagctacca cccggtccgg atcgtttacc tctgctgatg
      241 gctacggtgc tgaaaaaacg tattcgcttg caaggattta ttatcgctca ggattatggt
      301 caccgcatcc aagagtttca gaaggagatg gggcaatggg tgaaagagga taaaatccac
      361 taccgcgaag aaattactga cggtttagag aatgcgccac agacgtttat cggcctgctg
      421 aagggtaaaa acttcggcaa agtggtgatc cgcgtggcgg ttgatgatta aagtttcaac
      481 ggcggcgtaa gccgccataa aaatccaggc aaaaagctta ttttcagctt taattaacta
      541 accacgtcat tgttctgctg ctaataacaa caaaggtgag tggtttatga ttattcactt
      601 taatacacca ggtgaactcc ttctgccatg caggcagggt tggacagaaa acacgcgaag
      661 gaattaaaat gcctggaacg gaaaaaatga aacatgtcag tttgactctg caggttgaga
      721 acgacctgaa acatcagctt agtattggcg cattaaaacc tggcgcacgc ctgattacta
      781 aaaatctggc ggagcaatta ggtatgagta ttacacctgt gcgtgaagca ttattacgtc
      841 tggtttcggt gaatgcgctt tctgtcgcac cagcgcaagc atttacagtt ccggaagtgg
      901 ggaaacgtca attagacgaa atcaatcgga tccgctacga gctggaatta atggcagtta
      961 ctctggctgt tgaaaacctc accccgcaag acctggcgga actcgaggaa ttgctcgaga
     1021 aattacagca ggcgcaagaa aagggcgaca tggaacaaat cattaatgcg aacaggctat
     1081 ttcgcttagc gatttatcat cgttcaaata tgcccatcct gtgtgagatg attgagcaac
     1141 tgtgggtcag gatgggacct ggtttacatt atctctatga agcgattaat ccagcggaat
     1201 tacgggagcg tatagaaaac tatcgtctat tactcgccgc gttaaaagca aaagacaaag
     1261 agggatgcag acattgtctt gctgaaatta tgcaacaaaa tattgctatt ttataccagc
     1321 aatacaatcg ttaaattaaa tgcccggcaa ataccgggca tcaaggtaat tactcaaatc
     1381 gccacgcaat attcatgccg acaccataat ttcgcccggg cgcaggttcg taatatcg
//
LOCUS       DAZKGF010000393         1437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZKGF010000393 DAZKGF010000000
VERSION     DAZKGF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.396"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1437
                     /locus_tag="ACHOQV_005221"
     CDS             <1..>1437
                     /locus_tag="ACHOQV_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9633426.1"
                     /translation="VRTLAPDLDISIPYATDPAGNRLPDPELHPDSTLTVWPDNRIAE
                     DAHYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVE
                     SRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDSTRIQTV
                     YQPGSFTPLIRIETDVTELEKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLDRLEEE
                     IRTDRVSSESRAWLAQCGLTVEQLEKQVEPEYTPARKVHLYHCDHRGLPLALISEDGN
                     TVWSAEYDEWGNLLNEENPHHVYQSYRLPGQQHDEESGLYYNRNRYYNPLLGRYITQD
                     PIGLAGGWSLYAYPLNPVNGIDPLGLSPADVALMRKKEQLNHQRAWDILSDTYDDMKR
                     LNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNMFGMYGRKVKLSH
                     SEMIEDNKKDLAVNEHGLTCPLTQDCSNR"
BASE COUNT          375 a          338 c          455 g          269 t
ORIGIN      
        1 gtgcgcaccc tcgcaccgga cctggatatc agcatcccgt atgccaccga cccggcggga
       61 aaccggctgc cggacccgga gctgcacccg gacagtacac tcacagtgtg gccggataac
      121 cgcatcgcgg aggatgcgca ctatgtctac cgccacgatg aatacggcag gctgacggag
      181 aagacggacc gcatcccggc gggtgtgata cggacggacg acgagcggac ccaccactac
      241 cactacgaca gccagcaccg tctggtgttc cacacgcgga tacagcatgg cgagccactg
      301 gtcgagagcc gctacctcta cgacccgctg ggccgccgga cgggaaaacg ggtatggcgg
      361 cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta aaccggaggt gacgtggtac
      421 gggtgggacg gcgacaggct gacgacggtg cagactgaca gcacgcgtat ccagacggtg
      481 taccagccgg gaagcttcac accgctcatc cgcatcgaaa cagacgtgac cgaactggag
      541 aaagcgcagc ggcggagcct ggcggagaag ctccagcagg aagggagcga ggacggacac
      601 ggcgtggtgt tcccggctga actggtgcgg ctgctggaca ggctggagga agaaatccgg
      661 acagaccgcg tgagcagtga aagccgggcg tggcttgcgc agtgcgggct gacggtggag
      721 caactggaaa aacaggtgga gccggaatat acaccggcgc gaaaagttca tctttaccac
      781 tgcgaccacc ggggcctgcc gctggcgctt atcagcgaag acggcaatac ggtgtggagc
      841 gcggagtatg atgaatgggg aaacctgctt aatgaggaga acccgcatca tgtgtatcag
      901 tcataccgtc tgccggggca gcagcatgat gaggagtcgg ggctgtacta caaccggaac
      961 cggtactaca atccgttgct gggacgatat attactcagg acccgattgg gctggcgggg
     1021 ggatggagtc tgtatgcgta tccgctgaat ccggtgaatg gtattgatcc attaggatta
     1081 agtcctgcag atgtagcgtt aatgcgaaaa aaagaacaat taaaccatca aagagcatgg
     1141 gatatattat ctgatactta tgatgatatg aagagattaa atttaggtgg gactgatcaa
     1201 tttttccatt gtatggcatt ttgtcgagtg tctaaattaa atgacgctgg tgttagccga
     1261 tcagcgaaag ggctgggtta tgaaaaagag attagagatt acgggttaaa tatgttcggt
     1321 atgtacggca gaaaagtaaa gttatcccat tctgagatga ttgaagataa taaaaaagac
     1381 ttggctgtaa atgaacatgg gttgacatgt ccattaacac aagattgctc aaataga
//
LOCUS       DAZKGF010000394         1423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZKGF010000394 DAZKGF010000000
VERSION     DAZKGF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.397"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..328)
                     /locus_tag="ACHOQV_005222"
     CDS             complement(<1..328)
                     /locus_tag="ACHOQV_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFP9633427.1"
                     /translation="MNIFRTNFSRNTSDHLSKKAAYTEAIPYKTGIGIGLPLELEAIH
                     DAPASFFVSAHTHTSTMVGCMELPSGRLVSSNTSKANSVQSTASKIGLLGGGYNPKVR
                     GAFHMAV"
     gene            435..638
                     /locus_tag="ACHOQV_005223"
     CDS             435..638
                     /locus_tag="ACHOQV_005223"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633428.1"
                     /translation="MSKHRFSPVTPNISALQRQRLIRDIIETRKEKLTPPEKTSEPPP
                     LEVFLSGRKNQRNKLQQQKPSSL"
     gene            complement(644..868)
                     /locus_tag="ACHOQV_005224"
                     /pseudo
     CDS             complement(644..868)
                     /locus_tag="ACHOQV_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            963..1172
                     /locus_tag="ACHOQV_005225"
     CDS             963..1172
                     /locus_tag="ACHOQV_005225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633429.1"
                     /translation="MDNTQHTSQSENKKDDLTAYEQHFVEKKQSITRHIADMDMRKRF
                     EKTFNTNFALEDFLKQHSSRSSNKG"
     gene            1176..>1423
                     /locus_tag="ACHOQV_005226"
     CDS             1176..>1423
                     /locus_tag="ACHOQV_005226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633430.1"
                     /translation="MDDQKKNSFAMMVTLTSARITEHERKRKAAREQARATELTRKEL
                     NALAREHREAPEAFALDNFLKSTCRKKIRTSGPRGHSS"
BASE COUNT          471 a          337 c          317 g          298 t
ORIGIN      
        1 atacagccat gtgaaaggct cctctgactt tgggattata cccaccgccc aagaggccaa
       61 tcttgctggc ggtagactgt acggaattgg ccttactggt gttagaggaa accagccttc
      121 ccgaaggaag ttccatacag cccaccattg ttgaggtgtg cgtgtgcgcc gatacaaaaa
      181 aagacgctgg cgcgtcgtgt atcgcctcta actccagcgg gaggccaatc ccgatccccg
      241 ttttataagg gatagcctca gtatatgccg cttttttgct taagtggtcg gatgtgttgc
      301 gagaaaagtt tgtgcgaaaa atattcacat atccttccag aaaggatgga taccagaaag
      361 aaagacaacg taccagacaa taaccagcgc cgtgatatga ggcatcacga cataatgcat
      421 taccggaatc cagtatgagt aagcacagat tttcgcccgt taccccgaat atcagcgcac
      481 tacaacgcca gcgactgata cgtgacatca ttgaaaccag gaaagaaaaa ttaaccccgc
      541 cagaaaaaac cagcgaaccg ccaccgcttg aggtgtttct cagtggcaga aaaaaccaac
      601 gcaataaatt acaacaacag aaaccatcat cgctgtagcg tggtgtgtta cggcgtattg
      661 ctttacaggt acagacaatg acaaatgaag aactacagga aatcgcatta tccatacttg
      721 agcgacagcg cgaaaaagca caacggcaaa gaaggttgtt acttaatcca cagccactca
      781 ccaggccaac acgatttgcc cttgaggctt ttgtcgagaa atacccacgg cggttaaaag
      841 ccactaaaaa ccgaccgcca ggctgatagc tcgctgtttc ttctgcggct ctatgaaaca
      901 gacagaaacc atcagcgccg tgatgtgagg aagtacggcg cattgtttta caggtgcaca
      961 atatggataa cacacaacac acttcacaat cagagaataa aaaagatgat ctcacagcat
     1021 atgagcaaca tttcgttgaa aaaaaacagt caataacgag acacatcgca gatatggata
     1081 tgcgcaagcg atttgaaaaa actttcaata ccaattttgc gcttgaggat tttttaaagc
     1141 agcattccag ccgatcaagc aataagggat agcttatgga tgaccagaaa aaaaactcct
     1201 ttgccatgat ggttacactg accagcgccc gtataactga acatgaacga aagcggaaag
     1261 ctgcacgcga acaggcaaga gcaacagaat taacacgtaa agaacttaac gctctggcac
     1321 gggaacacag agaagcacct gaagcttttg cacttgataa ttttctgaaa agtacatgcc
     1381 gtaagaaaat acgaacatcg ggaccaaggg gccattcgtc gta
//
LOCUS       DAZKGF010000395         1397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZKGF010000395 DAZKGF010000000
VERSION     DAZKGF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.398"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..873
                     /locus_tag="ACHOQV_005227"
     CDS             <1..873
                     /locus_tag="ACHOQV_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP9633431.1"
                     /translation="AFDQPYWMMKASLPAVLNKVITDVGPGKLNLGGCVIEVMGTYNA
                     AIVRVTIGEYGATGFINGTVCTCTVYGDTQYFHWRVDYSTKNKPDTVSQRDASTTQKG
                     VVQLSSDTGSNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDPGAQ
                     GPKGETGATGPAGPRGAQGPKGEKGDPGPRGERGETGPMGPPGASDGKSRVVGIRLGN
                     EQTYRAEESSGFLSISLSIGGVITGLKSRGADGVISVSYRPLQVTFDGSQWRTVSVGE
                     YVSSGASGFEYFPL"
     gene            915..>1397
                     /locus_tag="ACHOQV_005228"
     CDS             915..>1397
                     /locus_tag="ACHOQV_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9633432.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGISRVASVLWPVGQSVVEVADTEENRKADISGRWGFDGEKITGLLTAEK
                     ARGMKGDEINAWRNAMEAANYMFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     T"
BASE COUNT          364 a          287 c          479 g          267 t
ORIGIN      
        1 gcatttgacc agccttactg gatgatgaag gcatcactgc ctgcggtact caataaagtc
       61 atcacggatg tcggaccggg aaaactcaat ctgggtggct gtgtcattga ggtgatgggg
      121 acgtataacg cagccatagt ccgggtcacc attggcgaat acggtgcaac ggggtttatt
      181 aatggcacgg tctgtacctg tacggtttac ggcgacacac agtatttcca ctggcgggtg
      241 gattacagca caaaaaacaa accggatacg gtcagccagc gggatgccag cacgacgcag
      301 aaaggtgtgg tgcagttaag cagtgatact ggcagtaatg acgaaacaaa ggcagccacg
      361 ccgaaggccg tgaaggcggc aatggatgtg gcaaatgaag cgaaaacaaa ggcagaagag
      421 gctgcagcag gaggtggtgt tcccggtccg aaaggagaga aaggcgaccc cggagcacag
      481 ggtccgaaag gtgaaacggg agcaacaggc cccgctggtc cgcggggagc gcagggaccg
      541 aaaggggaga aaggcgaccc cggtcccaga ggtgagcgtg gagaaaccgg gccgatgggg
      601 ccgcccgggg catcagacgg gaagagccgg gttgtgggta tcaggctggg taatgaacag
      661 acttacagag cagaagagag ctctggtttt ctgtcaataa gcctgagtat aggaggtgtg
      721 attactggtc tgaaaagtag aggagcagat ggtgtgatat cggtttctta caggccgctt
      781 caggtgacat ttgatggttc tcagtggcgt acagtcagcg tgggtgagta tgtgtcgtca
      841 ggagcttcgg ggtttgagta ttttccatta taaagcgcat catctttcag acatgtaacc
      901 gggaggactt acagatgcac ataaagaatt tcagacaata cacgccggaa aatccggatg
      961 tgccgggtgc gatgtacctg aaatcagagg atggtcagga ctggtatgag tgtcagtcgt
     1021 tattttcagc agagacgctg aaggtggttt ataacagtgc cggggtcatt accggcatca
     1081 gcagggtggc atcagttctg tggccagtgg gccagagtgt ggtggaggtg gcggatacgg
     1141 aagaaaaccg caaagctgac atctcggggc gctggggttt tgacggggag aaaatcacgg
     1201 gcctgctgac cgcggagaaa gcgcgaggga tgaagggcga tgaaattaac gcctggcgta
     1261 atgcgatgga agcggcgaac tacatgtttg agcacaatgg gcgtaaatgg gactacggga
     1321 agtcaacgca gacgcgtctt gagccgtcgg tggcggcagc gaaagcgggg aaactgccgg
     1381 aggcgttttt ctggacg
//
LOCUS       DAZKGF010000396         1347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZKGF010000396 DAZKGF010000000
VERSION     DAZKGF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.399"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1347
                     /locus_tag="ACHOQV_005229"
     CDS             <1..>1347
                     /locus_tag="ACHOQV_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9633433.1"
                     /translation="AQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDIRTDAR
                     LDEETVAFLTTTDEGKRINSHLYHDEERRIRLEKLARHKHENPPTLVSEAMAELRALP
                     PFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVNERFAT
                     PAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSAALERACDEVIEKTGRGDDD
                     LFTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRTAPDPEQVPGAVMRLSCATWWNS
                     QLRHLRDIWREELLRAAGLVSRNTSVYISHEALLDWREKRQRTRDFLKAWDIENEEGE
                     RFSLEDIYWAGLSNPRNRRNEMMACVRGMEQVAEARGDLAFFVTVTAPSRFHSVNDDG
                     SLNPKYCGATVRDASDYLVYRFFAAVRRAINKAGLGWYGVRTVEPHHDGTPHWHMLV"
BASE COUNT          298 a          419 c          389 g          241 t
ORIGIN      
        1 gcacaactgg cgtacccgtg gaacgccccg ctaccggtta tcgatcctga aacgttcggc
       61 agagcgcagc caaccgcatt acgccaggcc attcagagct acatcaacga ggatattcgc
      121 actgacgcca gactggatga agaaaccgtc gccttcctga caaccacaga cgaagggaag
      181 cgaattaatt cgcatcttta ccacgacgaa gagcgtcgga tcaggctgga aaaactggcg
      241 cggcacaaac acgagaatcc gccaacactg gtatctgaag ccatggcaga gctgcgcgcc
      301 ctgccgccgt ttcttgccgg tccgctcatc cgtgacctgg cgcgcctgag ccgcaaacag
      361 gagagcgcac gccgggaagg gcttaagaat aacagcgcat atgcggcgga taacttcgtt
      421 cgtcacgggc tgcgtgcccg cctgaaacgt atcgcccgtg tgaatgaacg ctttgccacc
      481 ccggccttta aggccaccgc cgcccgcgaa cggctggatg aactgcttat gctgccgcag
      541 ctgagccgtg acgaaatcca gcgccttgcc accctgaccg ccaccgcgtt ttccgccgca
      601 ctggagcgcg catgtgatga ggtcatcgaa aagaccggca ggggtgacga tgacctgttc
      661 acctggttac tgacctacca gccactggca cacatggcgt taaaactggg tatcacgcca
      721 ccctgctggc cgtcgctgga aatcagaccc gaccgccgca ccgcccccga cccggaacag
      781 gtgccgggtg cggtcatgcg cctttcctgt gcgacatggt ggaacagtca gttaagacac
      841 ctccgggaca tctggcgtga agagctgtta cgcgccgcag gtctggtgtc acgtaataca
      901 tcggtatata tcagccatga agcgttactg gactggcgcg aaaaacgcca gcgcacccgc
      961 gattttctga aggcctggga catcgagaac gaagagggag aacggttcag ccttgaggat
     1021 atctactggg caggtctctc taatccgcgt aaccgccgta acgagatgat ggcctgcgtc
     1081 cggggaatgg agcaggtcgc cgaagctcgc ggcgacctgg cgtttttcgt caccgtcaca
     1141 gccccttccc gttttcacag cgtgaacgat gacggcagcc tgaacccgaa atactgcgga
     1201 gcaacggtac gtgatgccag tgattatctg gtttaccgct ttttcgccgc cgtccgcagg
     1261 gccatcaaca aggccgggct gggctggtac ggcgtgcgca ccgtggagcc ccaccacgac
     1321 ggtacaccgc actggcacat gctggta
//
LOCUS       DAZKGF010000397         1339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZKGF010000397 DAZKGF010000000
VERSION     DAZKGF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.400"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..299
                     /locus_tag="ACHOQV_005230"
     CDS             87..299
                     /locus_tag="ACHOQV_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633434.1"
                     /translation="MISKYTTNARKPGKDARHHIAVLRNLLLEEIDDFTATLSGPGAP
                     VTPGEIHRDLKRRIENAIDYTLNPTE"
     gene            325..591
                     /locus_tag="ACHOQV_005231"
     CDS             325..591
                     /locus_tag="ACHOQV_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9633435.1"
                     /translation="MTILDYIAANPGCSGSEIATALNTQTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEARA"
     gene            588..878
                     /locus_tag="ACHOQV_005232"
     CDS             588..878
                     /locus_tag="ACHOQV_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633436.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDEL
                     EHARTTTPAIRLTLHHEIADFCATLEAPGEPETPEAIQQELLQRIDKVFDFF"
     gene            1209..>1339
                     /locus_tag="ACHOQV_005233"
     CDS             1209..>1339
                     /locus_tag="ACHOQV_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP9633437.1"
                     /translation="MANRKQQRARAERLHIHTEINRRLHRAASVSFIMQSNVLHRLN"
BASE COUNT          359 a          405 c          324 g          251 t
ORIGIN      
        1 accgcgattg ccagtaccgt tcgcgaagta atgaccatca gaggggcatg gaaatcagaa
       61 acacaagccc acggagagct gcggccatga tcagcaagta caccaccaac gcacgtaaac
      121 ccggtaaaga cgcacgccac cacatcgccg tactccgcaa tctgttactg gaggaaatcg
      181 acgacttcac ggcaacgctg agcggtccgg gcgcaccagt aacaccaggt gagatccacc
      241 gggaccttaa acgccgtatt gaaaacgcca ttgattacac cctgaacccg actgaataac
      301 cgaaacagca aaggagatct cacgatgacc attctggact acatcgcagc caatccggga
      361 tgcagcggaa gtgaaatcgc cacggcactg aatacccaga ccacagccat taatgctgag
      421 ttacgccgac tctggcgcgg cggcttagtc atcagaacaa accgcagcac aggtggtcgc
      481 gctcgcaaaa caggaggcca ggcttcttac cacgtaaacc cgatgccgtt cgggtgtagc
      541 aatccactta ctcacatgtt taaccagcta ctgaaggaag ccagagcatg agcaccatca
      601 accaccagaa gctacgcgaa ctggcatttg ccctgcaacg aatggcaacg cctcaaaaat
      661 tactggcatt tcgcgcaatg ctctcgccgt ctgctgtgct ggcactgctg gatgagctgg
      721 agcacgccag aaccacgact cctgccattc gcctgacgct ccatcatgaa atcgctgatt
      781 tctgcgcgac gttggaggcg ccaggtgaac cggaaacgcc ggaagcaata cagcaagagc
      841 tgctgcaacg cattgacaag gtttttgatt ttttctgaac cagtaagaaa ccagaacatg
      901 cacacacaaa aaaaccgctt gccagcgttc gcctggtcgg gttacagttt tcccgcacct
      961 cataaaacgg gtgccgggat tgcaaccccc gctgactaca caagcgcaca accgcgccag
     1021 cggttttttt gtgcgtaccg catcgtcaca tctttttcgc gtcagaatta tggtggggcg
     1081 tacagggccg acttcggtcg ggccggggtc ttgtgtagcc ggtggttgca accctgtacg
     1141 tctcaccacc cagaattgca acctctggat ggtgagtttt caaaacttac tacacaagag
     1201 gccacaccat ggcaaaccgc aagcaacagc gcgcccgcgc tgagcgtctt cacatccaca
     1261 ccgaaatcaa ccgcagactc catcgggctg catctgtctc cttcatcatg cagtcaaacg
     1321 tattacaccg acttaacga
//
LOCUS       DAZKGF010000398         1331 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZKGF010000398 DAZKGF010000000
VERSION     DAZKGF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.401"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..299
                     /locus_tag="ACHOQV_005234"
     CDS             87..299
                     /locus_tag="ACHOQV_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633438.1"
                     /translation="MISKYTTNARKPGKDARHHIAVLRNLLLEEIDDFTATLSGPGAP
                     VTPGEIHRDLKRRIENAIDYTLNPTE"
     gene            326..880
                     /locus_tag="ACHOQV_005235"
     CDS             326..880
                     /locus_tag="ACHOQV_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9633439.1"
                     /translation="MRLTPMGTPSKAPAHVKPWTQQEDRLLISLYQEHNTHQFAERIQ
                     RTRKAVMHRIRFLRNRGLIGRKRKAPLSTEVIAFLIKNRHVKTAQEMALEAGCSECTV
                     RYTLRKRGYNLQKCGENHHCARYSDHLVELVTELRDIHNMTFCMIAEHINSTTQMHIN
                     DDTAFHLYHRRTATDAVLRELLPG"
     gene            1201..>1331
                     /locus_tag="ACHOQV_005236"
     CDS             1201..>1331
                     /locus_tag="ACHOQV_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP9633440.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLHRAASVTFIMQSNALHRLN"
BASE COUNT          369 a          384 c          323 g          255 t
ORIGIN      
        1 accgcgattg ccagtaccgt tcgcgaagta atgaccatca gaggggcatg gaaatcagaa
       61 acacaagccc acggagagct gcggccatga tcagcaagta caccaccaac gcacgtaaac
      121 ccggtaaaga cgcacgccac cacatcgccg tactccgcaa tctgttactg gaggaaatcg
      181 acgacttcac ggcgacgctg agcggtccgg gcgcaccagt aacaccaggt gagatccacc
      241 gggaccttaa acgccgtatt gaaaacgcca ttgattacac cctgaacccg accgaataac
      301 cggaacagta acggaggtta tcgcgatgag acttacacca atgggaacgc caagcaaagc
      361 tccggcacat gtaaagccgt ggacacaaca ggaagatcgg ttactcatct cgctttatca
      421 ggaacacaac acacatcagt tcgccgaacg aattcagcga acccgtaaag cagttatgca
      481 ccggattcgt tttttacgta atcgtgggct gattggccgc aagagaaaag caccactgag
      541 tacagaagtc atagcctttc ttattaaaaa ccgtcatgta aaaaccgcac aggaaatggc
      601 tctggaagcg ggatgcagtg agtgcacagt gaggtacaca ctgcgtaaac ggggttacaa
      661 cctgcaaaaa tgcggtgaga accaccattg cgccagatac agcgatcatc tggttgagct
      721 ggtcacggag ctacgcgaca tacacaacat gacgttctgc atgatcgctg aacacatcaa
      781 cagcacgaca caaatgcaca ttaacgacga tacggcgttt catctctacc accgccgcac
      841 cgcaactgac gcagtgctcc gtgaactgtt accgggctga gtgatcatca tgcacacaca
      901 aaaaaaccgc ttgccagcgt tcgcctggtc gggttacagt tttcccgcac ctcataaaac
      961 gggtgccggg attaccaccc cgccgactac aagagcgcac aaccgcgcca gcggtttttt
     1021 tgtgcgtact gtatcgccac gtctttttcg cgtcagaatt atggcggggc gtacggggcc
     1081 gacttcggtc gggccgggat ctcttgtagc cggttgtggt aaccctgtac gtctcgccac
     1141 cctgagatta ccgcctcggg ttggtgagtt ttcagaactt actacaagag gccaaacacc
     1201 atggcaaacc gcaaacaaca gcgcgcccgc gctgagcgtc ttcacatccg cactgaaatt
     1261 aaccgcagac tccatcgggc tgcatctgtc accttcatca tgcagtcaaa cgcattacat
     1321 cgacttaacg a
//
LOCUS       DAZKGF010000399         1331 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZKGF010000399 DAZKGF010000000
VERSION     DAZKGF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.402"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            30..932
                     /locus_tag="ACHOQV_005237"
     CDS             30..932
                     /locus_tag="ACHOQV_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFP9633441.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRSNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGGDETAHSFRYSYAASAMKYHIMNGYSYKEALALTS
                     IDLGHGDGRGRYIEQVYCQLLGYE"
     gene            965..1288
                     /locus_tag="ACHOQV_005238"
     CDS             965..1288
                     /locus_tag="ACHOQV_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633442.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADYFDVVGDFNCDVRWSGRKILIKGTCVFPLGEPVKGIHNIPL
                     WIDVE"
BASE COUNT          425 a          207 c          330 g          369 t
ORIGIN      
        1 gtaaaaatta ttatgtgcag gagaagttga tgtccagacg tgaaaaatta ctgaaacagg
       61 atctggttgc tcacattcga aaagacaaag gcagcttcaa gaagctgagt gattacacca
      121 ggatcatgca ccgctttgct gaaagtctgg caaaaatgaa tgtacagatc agttctgccg
      181 cgcaggtaaa agtccggcat attgagctat atatgagaag ccggagtaat gtttctgggc
      241 gaacaaggga aaatgaaatg tctgcaatac gggttatgtt acgtcaggca ggtaaatata
      301 agatggctga tcccgaacat cctcgtctca gcaataaagc gttaggcatt tccggctcca
      361 gtcgtaaggg aacaaaaaaa ttaatcacgg atgagcgttt tcgggagatt cttcagcgtg
      421 tggaagcaaa agataaaggg gtggctgctg ttgttaaact gtcacgttac ctcggtctga
      481 gaaatgaaga agctatccag tcggccaaat cattacgaac ctggaaaaaa gcgttacaac
      541 ggggagatga aaaaataaga gtgatttttg gtacgaaagg gggacgggaa agaatgacaa
      601 cggttgttga ccgggataac gttcttgatg ctgttaatta tgcactcaaa tatgccagtg
      661 aacataatgg acgtcttata gacagagata atcttaagtc ggcaatggat tattatctta
      721 atacattacg tcgttatgga gaactgaaag gcggagatga gactgcacac agttttcgat
      781 acagttacgc tgcaagtgcc atgaaatatc atattatgaa cggttacagt tataaggagg
      841 cacttgcact gacatcaatt gatcttggtc atggtgatgg cagaggacga tatattgaac
      901 aggtttattg tcagttgtta ggctatgagt aataactcat ggcgatttcg tacgggtaaa
      961 tattatggat tgtactattt tgctgaaaat aatctctgta aaagagatta caaaaaattc
     1021 gcctttgtgg tcacactatc tggcgcgata tgttgattcc ggatatcagg ccgagtttga
     1081 atgtgaagga acagcagata aatgggatat ctatgatgaa tgggacatct atgaatccat
     1141 tgcggattat tttgatgtgg taggagattt taactgtgat gtcagatggt cgggaagaaa
     1201 aatactgatt aaagggacct gtgtttttcc tctgggagaa cccgttaagg gaatacataa
     1261 tattcctctt tggattgatg ttgaataact tgacattcat cttttgtgat ttaaaaatat
     1321 acaggcttat a
//
LOCUS       DAZKGF010000400         1330 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZKGF010000400 DAZKGF010000000
VERSION     DAZKGF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.403"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            349..612
                     /gene="ykgM"
                     /locus_tag="ACHOQV_005239"
     CDS             349..612
                     /gene="ykgM"
                     /locus_tag="ACHOQV_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9633443.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            612..752
                     /gene="ykgO"
                     /locus_tag="ACHOQV_005240"
     CDS             612..752
                     /gene="ykgO"
                     /locus_tag="ACHOQV_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFP9633444.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(787..1012)
                     /gene="ykgL"
                     /locus_tag="ACHOQV_005241"
                     /pseudo
     CDS             complement(787..1012)
                     /gene="ykgL"
                     /locus_tag="ACHOQV_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
BASE COUNT          376 a          294 c          279 g          381 t
ORIGIN      
        1 ttcacttcag tgatatttaa aatatgcatc ctctcccttt tttgtaagta attattatat
       61 ccgtgggaga ggaatacaca ttgtcaggta atcaatcatg ctgcaataaa tcatcggcca
      121 gtaaagtgga gatagcctcc attctcgaaa aatccatact ctcagcgaaa ccatcatcaa
      181 tcactcatcc aggcgtttat gggagcgtcg ccaatggctg ctaacaatgc cagacttccc
      241 cgttgcggaa attccacatc ccacaaatag tcacagtgat tgggtgttga aatgatccgg
      301 atgagcatgt atctttacgg ttatgttata acataacagg taaaaatgat gaaacccaat
      361 atccatcctg agtatcgtac tgtggtgttc cacgacacca gcgttgatga gtactttaaa
      421 atcggctcga ctatcaaaac agaccgtgag attgagctgg atggcgtaac gtatccgtac
      481 gtaacaattg atgtctcttc gaaatcgcac ccgttctata cagggaagct gagaacagtg
      541 gcatcagaag gaaatgttgc acggttcacc caacgttttg gtcgttttgt tagcacgaaa
      601 aagggggcat gatgaaagtc cttaactctc tgcgtaccgc aaaagaacgc catccagact
      661 gtcagattgt gaagcgaaaa ggacggctgt atgtgatttg taaatctaat ccacgtttta
      721 aggccgttca gggtcgtaag aaaaaacgtt gattcaaaat tcgacgaatt aacgatattt
      781 gtctgattaa taatcatacc ggattaatgt tggtgtattt attacaccag cattaatttt
      841 cctggggatg tatcttcctg ttaatttaag gtcaactgcc tgacgtttct ctccgaatat
      901 tccattatat taatgttgac ttgttgacca gctgcgccct gtatgctggc atcaactcta
      961 ttttagactg aacacgccac tcagtttcct ccctttgcga cgcagcctgc atttacactc
     1021 aaactgttaa gatgataaat gtggtaaatc tgttggtact gacataaaaa cgtttacgcc
     1081 acaggaacgg ccagatccat cggtaacccc atcgccgacg ttcgagtgcc agttagagta
     1141 acgcgtacag atgactgaat gcagtgccct ggcaaagagg ccatcgttcc tgtgacaact
     1201 ggcagtcttc gtttaacttc acttaatttg gctcttgggg ggcttaccgg acagatgacg
     1261 tacttacacc tgtttaattt ttcatcactt attgggatga acacccacaa ccattttgtg
     1321 cggcatgtta
//
LOCUS       DAZKGF010000401         1314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZKGF010000401 DAZKGF010000000
VERSION     DAZKGF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.404"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            25..759
                     /locus_tag="ACHOQV_005242"
     CDS             25..759
                     /locus_tag="ACHOQV_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9633445.1"
                     /translation="MKNIYKIIACAVIGTSMVAMPAHAAEGDHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHSRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVTADLKIKDNISSSGGFSESNSTKKNSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            991..1128
                     /locus_tag="ACHOQV_005243"
     CDS             991..1128
                     /locus_tag="ACHOQV_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9633446.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     K"
BASE COUNT          348 a          256 c          354 g          356 t
ORIGIN      
        1 tttaataaca ggaataatct aaaaatgaaa aatatttata agattattgc gtgtgcagtt
       61 attgggacga gcatggtagc catgccagca catgcagcag aaggagacca tacaatttct
      121 ctggggtatg cgcactttca gtttccggga ctgaaggatt ttgtaaagga tgcgactgct
      181 cataacaggg agactttcag tcatttcgtc aacagaaact acttttcttc attgggcgaa
      241 tatacagatg gtcgggtcag tggatatgaa ggcaaggata aaaatccaca gggcattaat
      301 atcaggtatc gctacgagat aacggatgat tttggcgtta tcacctcttt tacatggacg
      361 cgttctctca ctaactcaca gacatttatt gatgtgcagt cagccgatca tagcaggaag
      421 attaagaatc cggcagcttc tgccagaacg gatatcaggg cgaattactg gagtctgtta
      481 gcggggcctt catggcgggt taatcagtac atgagtttat atgcgatggc agggatgggc
      541 gttgctaaag ttaccgctga cctgaaaatt aaggacaata ttagcagtag tggcggattt
      601 tctgaaagca acagcacgaa aaaaaactcc cttgcgtggg ctgcaggtgc acagtttaac
      661 ctgaatgaga gtgttacact ggatgtggct tacgaaggtt ccggctctgg cgactggcgc
      721 acgagtggag ttactgctgg cattggcctg aaattctgat ctgtatccgg taaccgttta
      781 ctacccgctg tgatggcggg ttttttattg cccgtacagg gcaaaaaccg taaattatgc
      841 gtgggtgcct ttcggctgat ggctggaggg agaacctgaa ggcctgatgt ggaaaggccc
      901 cgagtcaact taacgttaac ccgaggccct aaccatcata ccttaagcaa gtagaaggtt
      961 agcgcctctc tgtaaaagga gtcaagcgct atgtcgcaaa aatcgcttat caccgtcaca
     1021 atttgtatga cggttatctt caccatctgg atgttacacg gctcgttgtg tgagttccgg
     1081 ctgaatttgt ggggagcgga gtttgcggcg ttcttacagt gtaagtagta ggaaaaccgc
     1141 gacggggacg aaagtccccg tcaactggtt gctgaggttc agtcgatatg gcacccgttt
     1201 gtagagtgtc tttcatccaa tcccggacag gtcgagagag gggatatgtg tgaatgggaa
     1261 ataataacta ttggaaatgt tttgtttttt ctaagtcttt ttggcggtgt gtat
//
LOCUS       DAZKGF010000402         1313 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZKGF010000402 DAZKGF010000000
VERSION     DAZKGF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.405"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(180..1118)
                     /gene="lrhA"
                     /locus_tag="ACHOQV_005244"
     CDS             complement(180..1118)
                     /gene="lrhA"
                     /locus_tag="ACHOQV_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFP9633447.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT          356 a          319 c          305 g          333 t
ORIGIN      
        1 ttcaggcgat tatttgtgcg ttcgggacgt gaatctctgg tgattgaaaa atgaacagtt
       61 ttgtacgttc tgcacgatac gaggaaggct ttttactaaa aaaaagccgc tggggtttaa
      121 aacaccccca gcggctcgtt tttacactat tgtctcagaa attatctatg gtccgtcgat
      181 tactcgatat ccctttcaat caacaatgag tcatcgcctt ccggagcaga cattgggctg
      241 tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc cagctcatta
      301 ttactggacg ggtcatagca cagcaaatat tcggtatcgg gtagcggcgg taagccatct
      361 actccactca gcacacgcag gtctggactc atcatctcga ccggccttgc cgtcacgcca
      421 agaccggctt ttactgccgc acgaactgcc ggaagcgtcg aggcgacata agccagtcgc
      481 catggaatat ctgctttatt aagcgtcgcc agcaccatat cgcgaaacgg gctaggatca
      541 tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc tgcgcagtac
      601 cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ttgaaggacg atgggtggtt
      661 accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt acgtttaacg
      721 cggacatcca gcgctagttt cggataaacc gaactcacgc gatttaacag gaaaggtaag
      781 atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag attactgaac
      841 attaatgatg agcaggcctc atcattaaaa cgcagaattt ttctggcgta accaagaagt
      901 tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag ttctttccca
      961 acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg acacacagcg
     1021 gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct caacagatcg
     1081 aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt actggcggct
     1141 catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg taatgtgcct
     1201 ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt tgcactaaaa
     1261 aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat atc
//
LOCUS       DAZKGF010000403         1289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZKGF010000403 DAZKGF010000000
VERSION     DAZKGF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.406"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            165..923
                     /locus_tag="ACHOQV_005245"
     CDS             165..923
                     /locus_tag="ACHOQV_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFP9633448.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(976..1083)
                     /gene="yniD"
                     /locus_tag="ACHOQV_005246"
     CDS             complement(976..1083)
                     /gene="yniD"
                     /locus_tag="ACHOQV_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFP9633449.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT          331 a          289 c          301 g          368 t
ORIGIN      
        1 tgttttcgcc acatgcttat tcaaaaaatg aaataagaat acattgctaa gttattcgcg
       61 cctttttata attcgttacc tttccaggga cgctaatctt tgatccttaa gaaagtttcc
      121 gggaccgcga aggtctcttt ttttgttgta cggactcttg ataaatgaag cttttgaaga
      181 cagttcctgc catcgttatg ttggcggggg gcatgtttgc gtcactgaat gcagctgccg
      241 atgattccgt ttttactgtc atggacgatc ctgccagcgc taaaaaacct ttcgaaggta
      301 atctgaacgc aggatatctt gcacaatcag gcaatacgaa aagctcctca cttacggctg
      361 acaccaccat gacctggtat ggacaaacca ctgcctggtc gctgtggggg aacgccagta
      421 atacttcttc taatgatgag cgttcttcag aaaaatatgc ggcgggcggg cgtagccgtt
      481 ttaatttaac tgattatgac tatttatttg ggcaggcaag ctggctgaca gaccgttata
      541 acggctatcg agagcgcgat gtgttgaccg cgggttatgg tcgccagttt ttgaatggcc
      601 cggtacacag tttccgcttt gaattcggtc caggtgtgcg ttacgacaaa tataccgata
      661 atgccagtga aacccagccg ctgggttatg cctccggggc ttatgcatgg cagttgactg
      721 acaacgctaa atttacgcag ggtgtttccg tctttggcgc ggaagacaca acgctgaact
      781 ctgaaagcgc attaaatgtt gctattaatg aacattttgg actgaaggtg gcttacaacg
      841 taacatggaa ctctgaacct ccagagtctg caccggaaca taccgatcgt cggacaacct
      901 tatcgctggg ctacagcatg taaaattatc tgccggaccg tgttatgcgg tctggctgtc
      961 ttcgtttgtc actgctcagc cccaaatcat tgctgcccaa cgtagcaaca acatagcgcc
     1021 acagattgcc agtagcacca ccaccatctt ccagtgtttt ttatcaaagc gtttagtcgg
     1081 cataccagtg tcctgcatat ctcttgtgcg tgataacgga gtgtaacaaa agggacaggc
     1141 atcaagggat tttttgttat tacagccgtc gggtggcgtt gtacacctga tcactgtttt
     1201 tgcggtttat gaaaaaaata aaattatgta tcgatatata aatatctggt tataaatacg
     1261 tctttagttc atcagcacta aaactcagg
//
LOCUS       DAZKGF010000404         1285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZKGF010000404 DAZKGF010000000
VERSION     DAZKGF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.407"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            112..1194
                     /locus_tag="ACHOQV_005247"
     CDS             112..1194
                     /locus_tag="ACHOQV_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFP9633450.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          379 a          270 c          281 g          355 t
ORIGIN      
        1 aatcaaacga gaaaccaaaa cttacatctt gaaataatca cattgattag atgaatattt
       61 atcgcgcagt gacatcattt tttaataata gttcaaaaaa aagggctcac gatgaaaaaa
      121 ttaacagtgg caatttctgc tgtagctgca tcagtactga tggcgatgtc tgctcaggca
      181 gctgaaattt ataataaaga cagtaacaag ctggatctat atgggaaagt taatgccaag
      241 cactacttct cctctaatga tgcagatgat ggtgatacta cttatgcccg tcttggcttc
      301 aaaggtgaaa cccaaatcaa cgatcaactg actggtttcg gtcagtggga atatgaattc
      361 aaaggcaacc gcgctgaatc tcaaggctcc tccaaagaca aaacccgtct tgcatttgca
      421 ggcctgaaat tcggggacta cggctcaatc gattacggcc gtaactacgg tgtagcatat
      481 gacatcggtg cgtggactga cgttctgcca gaattcggtg gcgatacctg gacccaaaca
      541 gatgtgttca tgactggtcg caccactggt gttgcaactt atcgtaacaa cgacttcttt
      601 ggtctggttg atggtctgaa ctttgctgct cagtatcagg gtaaaaatga ccgcactgac
      661 gtaactgaag ctaatggtga tggtttcggt ttctccacta cttatgagta tgaaggattc
      721 ggtgtaggtg caacctatgc taaatctgac cgcactaata atcaggttat ctacggtaac
      781 aacagcctga atgcatctgg tcaaaatgct gaagtatggg cagctggtct gaaatatgat
      841 gcgaacaaca tctatctggc taccacctat tctgaaaccc agaacatgac tgtttttggt
      901 aataaccata ttgccaacaa agcacaaaac ttcgaagtag ttgcacaata tcagttcgac
      961 ttcggtctgc gtccgtccgt tgcttacctg caatctaaag gaaaagactt gggtgcgtgg
     1021 ggtgatcagg acctggttga atatattgat gtaggtgcaa cctattactt caacaaaaat
     1081 atgtccactt ttgttgatta caaaatcaac ctgattgata agagcgattt cacgaaagca
     1141 tctggcgttg ctaccgatga tatcgttgct gtaggtatgg tttaccagtt ctaatttgat
     1201 tactaaaaga tatgttgcgg gaggctttgc ctccccaaca tataagtggc tccctcaagc
     1261 cacttccttt aggagcacaa ccttg
//
LOCUS       DAZKGF010000405         1264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZKGF010000405 DAZKGF010000000
VERSION     DAZKGF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.408"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..668)
                     /locus_tag="ACHOQV_005248"
     CDS             complement(<1..668)
                     /locus_tag="ACHOQV_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFP9633451.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIHELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDS"
     gene            complement(949..>1264)
                     /locus_tag="ACHOQV_005249"
     CDS             complement(949..>1264)
                     /locus_tag="ACHOQV_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633452.1"
                     /translation="QDNIALEQVVTWDSDNIFTPNIFEHNGRCFMKKNETWMLLNGDA
                     TPNIRTMSNKKIECLEKSEVEWASRVCDSITDKERENLKALICSKPAKSVNTGKRPAL
                     GY"
BASE COUNT          337 a          273 c          221 g          433 t
ORIGIN      
        1 taactgtcat gctctggatt atttaattct ttcagtggga atttttcctc aaaggcttta
       61 ttaagccgcc cctgatttcc ttttacagcg aataaataat caccgccctg tttttgtatc
      121 ttctctgcga tatctttctg gcaacccatc gcatcagttg tgataatttt tcctttaata
      181 tccaacatgt taagaagttc atggatagct gtgatctcat tggatttctc atccgtcctg
      241 atctgtccga tgaccagact gtgcattgtt gagaacgcac taatgacatg aatcgctccc
      301 ctgcggcgac tcttgtcata agaatgccgg agcgtttttc catcaattgc aataacgtct
      361 ttatcatctg aagaatggca gtcacgcatc cagttaataa agcactcgtg aaattttgca
      421 ggactgatac aggatacaac tctggcaatg gtatcgtgaa caggaatacc attttcaaaa
      481 tcaccatatt gcttcaaaaa atcgagatgt gtttccccaa aatcctctat atcttcccag
      541 ccttctgcac cagaaataac ggcacaaata gtcaacagta gaatatccga caatttatgt
      601 tccactttcc aggcttgtct gtaatcgggg ataatagaaa tatgctccat caatttttta
      661 agttccattt tgttctcctt aattatgtaa ggagtatttg atcatgtata agcaataaaa
      721 aacagcttca ggtaatgagg aatatctcaa tttttaaaca taaaatgcga attatttagt
      781 acaaaaaagc agcgttgcgt gatctttccc tagtccctgt cccggattgt agtaccggtg
      841 ccagttgtag tgcagttcgg attcgtcgtc atactgctag cccggatggc ggagcagttg
      901 ctctagtcac tcagaatatt gtaatagtga tagagtgcag tgccggtttc aatatcctaa
      961 cgctggtctt ttgcctgtat taactgattt agctggtttg ctacatatta atgccttcag
     1021 attttccctt tccttgtctg tgatactgtc gcaaacccta ctagcccatt ctacttcact
     1081 cttctccagg cattctattt tcttattgct cattgttcta atgtttggtg ttgcatctcc
     1141 atttaataac atccatgttt catttttctt cataaaacat cttccattat gttcaaagat
     1201 attaggtgtg aatatattat cagaatccca tgttacgact tgctcgaggg ctatattatc
     1261 ttga
//
LOCUS       DAZKGF010000406         1260 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZKGF010000406 DAZKGF010000000
VERSION     DAZKGF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.409"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            55..1104
                     /gene="ydeM"
                     /locus_tag="ACHOQV_005250"
                     /pseudo
     CDS             55..1104
                     /gene="ydeM"
                     /locus_tag="ACHOQV_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(1070..1192)
                     /locus_tag="ACHOQV_005251"
     CDS             complement(1070..1192)
                     /locus_tag="ACHOQV_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633453.1"
                     /translation="MTKHPTGIYVGCFVKVIRRRLRLEQRRSVINYSPFVLQHP"
BASE COUNT          387 a          247 c          247 g          379 t
ORIGIN      
        1 tggaattaag tacaataaga agaggaacat ttatgaagtc tgcattaaag aaaaagcact
       61 ctgaaagagt tcatcaaaca atatatcgca gcgtctggca atcaggtcta ttttacctgg
      121 caaggcggtg aacccactct ggctggcctg gattttttcc gtaaagttat tcactatcaa
      181 caacgctatg caggccaaaa acgtattttt aatgcattac aaacgaatgg cattttattg
      241 aataatgaat ggtgtgcctt tctcaaagaa catgagtttc tggttggtat ctcgatcgat
      301 ggcccccaag agctacatga ccgttacaga cgcagtaatt caggtaacgg tacttttgca
      361 aaagtgatag cagccatcga gcgtctgaaa tcatatcaaa tagagtttaa tacgttaacc
      421 gttattaata acattaatgt ccattaccct cttgaggttt atcatttttt aaaatctatc
      481 ggcagtaaac atatgcaatt tatcgaattg ctcgaaaccg ggacgccgaa tattgatttc
      541 agtggtcata ctgagaatac attccgtatc attgattttt ctgtgcctcc cacggcttat
      601 ggcaagttta tgtcaaccat ttttatgcaa tgggttaaaa acgatgtggg tgaaattttc
      661 atccgtcagt ttgaaagctt tgtcagccgt tttttgggga atgggcatac cagttgtatt
      721 ttccaggagt cctgcaagga taatctggtt gttgaaagta atggagacat ttacgaatgc
      781 gaccattttg tctatccaca gtacaaaatt ggaaacatta ataaatcgga actcaaaacg
      841 atgaacagtg tacaactgac agcgcaaaaa aaacggattt cagcgaaatg tcagcaatgt
      901 gtatataaac ctatctgcaa tggtggttgc cctaagcatc gtattactaa agtaaacaat
      961 gagactattt cctatttttg cgaaggttat aaaatccttt tttcaaccat ggtaccttat
     1021 atgaacgcca tggtggagtt agccaagaac agagtaccgc tttaccacat tatggatgtt
     1081 gcaaaacaaa tggagaataa ttaataacgc tccttctttg ctccagtctt agcctccgtc
     1141 ttatgacctt aacaaagcac ccgacataaa ttcctgttgg gtgtttcgtc atcatcatct
     1201 tcccttgcga aaattctggc ttacgcggca tgcgtcaggg cattagcaat acccttgatt
//
LOCUS       DAZKGF010000407         1255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZKGF010000407 DAZKGF010000000
VERSION     DAZKGF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.410"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(431..1063)
                     /locus_tag="ACHOQV_005252"
     CDS             complement(431..1063)
                     /locus_tag="ACHOQV_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFP9633454.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          331 a          269 c          257 g          398 t
ORIGIN      
        1 tatcgtcgta gctgcactcg atatttttaa attcctgtat caagtgctga taatcatttc
       61 atcgccactt ccaattcctt tctgcatggt aagtagtgag gacgaaaaga agaatatgat
      121 aaatattggt tcgcggtaat cagagagggc cgcgacattt gtgatgttat tcttacaact
      181 gtaagaagcg ccggtgatgt acaagtaagg gtatgattac gctttttaaa gcgtaccata
      241 aaacataaca taataaatga tttatgatga cacgggatta ttgcggttta ctttgaagaa
      301 tataaaatgc gagtttatag caccgcgagc gccaaataat tttatggcca caacctgtcg
      361 tattgtggcc ataaaaaatt gtcggctgga gggagtaaaa tcctttacct caatatattg
      421 cctgtcagct ttagttcaca aacagtgctt cgctatcgac gatgattcca tcttccgcac
      481 ctttaagcgc caggtttgcc tcgtagtatt gctttttatc aagcagtttc tgcgcatcag
      541 caagtgcatt acgagtctgt ttgagcggca tcagatattg gttttccatt acaccaacac
      601 cggccaggcg aagctcttcc attgcgccct ttttatcccc tttcgccatt ttttcgttgg
      661 caatttttat cgcggcttct ttttcgggtg tcgctacata actctcagaa attccgacag
      721 atgcattaat gacaatatat tggtcatcat tgagattggt ctttttacct ggcttagcga
      781 actttgccca ttcggtactg tcatctgaca acaaagctga agcttcattg gtcagttctt
      841 ttgccttttc cggatcgcca tgaaacagag ccaggcgcgc tacctgcacg tcacgcatcg
      901 catataaacc ttgttcagag atacgctctg ctgctgccgt tttctgggtt tgctgaactt
      961 gctgagtttg ctgttgtgcc gctaccggtg cattatcagc agcccataca gttgaagatg
     1021 ccagaattgc tgtgaccatc gtagccataa ttaaacgttt catacttacc tcattcttta
     1081 acagttttag aaaacgccgc catcatattg ggtgagcatg aaatgagaaa tcagagagca
     1141 acggtcattt tgaaagggtt atcttacagt tgtagttatt atcgcaagaa gtattttagt
     1201 cttacaattg taggaatatg cttcctgtca cagagaacta ttcttatata ccaca
//
LOCUS       DAZKGF010000408         1251 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZKGF010000408 DAZKGF010000000
VERSION     DAZKGF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.411"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..284)
                     /locus_tag="ACHOQV_005253"
     CDS             complement(<1..284)
                     /locus_tag="ACHOQV_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HFP9633455.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLL"
     gene            complement(352..>1251)
                     /locus_tag="ACHOQV_005254"
     CDS             complement(352..>1251)
                     /locus_tag="ACHOQV_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HFP9633456.1"
                     /translation="GQLLPGEAGAWLATVPGALQNLAGAVWHQHPEMALIRTVLQEAM
                     EEVVSPLAAGDVAEVPVAEVVPVLLQSAAISAESVIQPLPVVQSPVEDAENEGRTAEQ
                     EVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPDKESGIELPEESGCVTENQGN
                     RIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSLSVNSVDSNVHVVSGYVFLCAP
                     GIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFFQARLYSDAEHSGRYKMVSGY
                     LVKGRFLYGNITVPGDSPLIVFG"
BASE COUNT          302 a          398 c          238 g          313 t
ORIGIN      
        1 agcagtttca tcttgatccc gaatcccata attgcccgca agtgggccac cttgttattc
       61 caggtcacct ccctgcgctt ctgctctttc aggacataac gccggtatcg ctgaacatca
      121 cggacagtca cctcccccgg caaaatatca ccgataaatt ccctgaatac cctgacaact
      181 ttgcagtagc tccactctgt atccggccga agattctttg aaaaaaagta ctcttccagc
      241 agttgttccc aggtgatttt tgtgacggaa tcgtcattct gcattatata ttccccttgt
      301 ttactgtgct cccgcactct ccggtcaaat tctgcacatc atcgccttta ctcacccgaa
      361 aacaataagc gggctgtctc cgggaaccgt aatattcccg tacagaaacc tgcccttaac
      421 cagatatccc gaaaccattt tgtaacgtcc ggaatgttct gcgtcactat aaagacgggc
      481 ctgaaaaaaa cgctctcctg aacgtacctt atggatacct gcccgttcaa agagcgactg
      541 aacataacga cgatcctgcc cggaaccact ctccttcaga aaacgataaa aaatccccgg
      601 ggcacacaga aaaacatatc cggaaacaac atgaacattt gaatcaacgc tgttaaccga
      661 aagtgaaccc tccctcagcc cctcttttaa ccatctgata aattcactct ctaccctgct
      721 atcctctgtc agggaaaact ctgccgtttt ttccactgtt tccgggatac ggtttccctg
      781 attttctgtt acacatccgc tctcttccgg taactcaata ccgctttctt tatccggcgg
      841 cacgcctgac gctatctgcc ctccctcatc cgacaaatca gcagccgaaa acagcgtaag
      901 gagcattccg gtatcagcat cagtttcgtt cccctcatcc acctcctgtt ccgctgtccg
      961 gccctcattt tcggcatcct ccactggcga ctgtacaacc ggcaacggct gaatgacgct
     1021 ttctgctgaa attgctgctg actgcagcag taccggaaca acctcagcca ccggaacttc
     1081 ggccacatct cctgcagcga ggggactcac gacttcctcc atagcttcct gtaatactgt
     1141 acgtatcagc gccatttcag gatgctgatg ccagactgct ccggcaagat tctgtaatgc
     1201 ccccgggaca gtggcaagcc aggctcccgc ctctccgggc agcagttgtc c
//
LOCUS       DAZKGF010000409         1228 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZKGF010000409 DAZKGF010000000
VERSION     DAZKGF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.412"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1055)
                     /locus_tag="ACHOQV_005255"
     CDS             complement(<1..1055)
                     /locus_tag="ACHOQV_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9633457.1"
                     /translation="MAKSVITSAFIRLKAQQAAEGTPVVLDEFVFANVPDLNPETPVS
                     PDETLPPEDQIVHRQAVGKTGVVNENAVVYSVTLGAELGDFEFNWVGLVSKATSTLAM
                     IVHAPTQKKIRTHNGNEGNVLVRSMLMEYSGAKEATGITTPAQTWQIDFTARLAAMDE
                     RQRRENIDIYGNAAFFGDGWLVKKGERWSLCKGTGYVEGLRAELTEDIPLTTPDFPSR
                     IWLTVWWTGTLTSEWTVEHDIIMADPAATEEILLEAHRKEAARQYFFALADIDEDGNV
                     TDLRPRGTPEERRWLDALKKHEKSRNHPDATLKEKGFTQLSSATTSTSETQAATPKAI
                     KIAMDNADGRLAKNRNG"
BASE COUNT          221 a          346 c          336 g          325 t
ORIGIN      
        1 tcaccattgc ggtttttcgc aagccgacca tcggcgttgt ccatggcgat ctttatagcc
       61 tttggcgtgg ctgcctgtgt ctctgatgta ctggtggtgg cgctgcttaa ctgtgtgaag
      121 cccttttctt tcagcgttgc gtccgggtgg ttgcgtgatt tctcgtgttt tttcagagcg
      181 tccagccagc ggcgttcttc cggcgttcct ctggggcgca ggtcagtcac gttgccgtct
      241 tcatcaatgt cagcaagggc aaaaaaatat tgccgggcgg cctccttcct gtgtgcttcc
      301 agcaggatct cttccgtggc ggcggggtcg gccatgataa tatcgtgctc caccgtccat
      361 tcactggtga gcgtaccggt ccaccatact gtcagccaga tacgggacgg gaagtctggc
      421 gtggtcagcg gtatgtcttc ggtcagctct gcgcgtaatc cctccacgta accggtccct
      481 ttgcagagtg accatctttc gcctttttta accagccagc cgtccccaaa gaatgcagcg
      541 ttaccgtaaa tatcgatgtt ttcccggcgc tggcgctcgt ccatggccgc caggcgtgcc
      601 gtgaagtcta tctgccaggt ctgcgccggg gtggtgatac ctgtggcttc cttcgcaccg
      661 ctgtactcca tgagcatgga acggaccagc acattacctt cgttaccgtt gtgggttctg
      721 atttttttct gtgtgggggc gtgaacaatc atggccagcg tgctggttgc tttgctgacc
      781 agtcccaccc agttaaattc aaaatcaccc agctctgccc ccagggtgac cgaataaacc
      841 acggcattct cattcaccac gccggttttg ccaacggcct ggcggtgaac aatctgatct
      901 tcaggtggca gggtttcgtc gggggatacc ggtgtttccg ggttgaggtc cgggacgttt
      961 gcaaaaacaa actcatccag cacaacgggg gtgccttccg ccgcctgctg tgccttgagc
     1021 cggataaagg cgctggtaat tacagatttc gccataaagt catactcctt cagtcattca
     1081 ctctgccctg gccgtccagc agatatactc cccttcgtat gcgccataat ggatgatgaa
     1141 ggggttaagg gtttccacct caaaacggta acggcggcat gtcctgccat atttgcggat
     1201 gatctccaga agaagatcgc tgttgtcg
//
LOCUS       DAZKGF010000410         1224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZKGF010000410 DAZKGF010000000
VERSION     DAZKGF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.413"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(18..245)
                     /gene="mchB"
                     /locus_tag="ACHOQV_005256"
     CDS             complement(18..245)
                     /gene="mchB"
                     /locus_tag="ACHOQV_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFP9633458.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALARIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            complement(273..482)
                     /gene="mchI"
                     /locus_tag="ACHOQV_005257"
     CDS             complement(273..482)
                     /gene="mchI"
                     /locus_tag="ACHOQV_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFP9633459.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRRILVWSGWKNGS"
BASE COUNT          415 a          258 c          225 g          326 t
ORIGIN      
        1 aataaataaa aatataatta gctaccgcca ccagcagaag aactggcact accacttccc
       61 acggtcgagc caattgctgt tgtcaaaccg gcagatacgg ctccaactac aaccccaagt
      121 ggaccaccag gtattctggc gagagctcca acaatagctg cagcacctgc agcattagct
      181 gaagtcgctg gcgcacctcc cgccccggaa atatatctta actgtgattc tgttatttct
      241 cgcatataaa ctgcatataa actccatcat atttaacttc cgtttttcca gcctgaccag
      301 accagaatcc ttcgaatccc atgaatcagc tcaataaaac caagaaatat tataaagctt
      361 agaaaaacgt caacataaga attcccttcg ccaacaatcc gaagggatga aagtgaaacc
      421 aataagatag taataggtaa actgaatata gccgtcaatt gggacagttt tttataactc
      481 attaatccct acctgttgct ttattttcat tatgaaaagt taccaaatct tagcacctca
      541 gtcaggagag tcaggtcatc gataaagaat attccagtag agtggcgctc atgatcaaag
      601 ataacagcaa agttgatgac agcgctgacc gactgtcatt tacagaaacc ctgtttgtag
      661 cacattccat cttacctgac ccagacaaag acaaagacaa aagagaatga ttgttattta
      721 cttttcaaaa aaaagtgtca tattgtccat ataaagtcaa gaggtatttt acgttatgtc
      781 actgatgcaa ttgttagtca ggatctcatg gttgtcgctc atgtataacc tgagacttga
      841 acgcacacta atagcactga cggtcataca tcgggaaatg tgaggaggca taagaatgaa
      901 ttgtgataat aatcacagaa atgaagaatt cattgttacc tttgataaag gcaacaagca
      961 agacaattca agacgaaaac acgataattt tcctatagag gtagaatcct ccgtagagca
     1021 ggagacacac tgtatcacaa ataataagtc ggcttccggt atagtaacac atgactatga
     1081 tgccgattat atttgtggtt gtggtgaaat tatgtgtcct ggttgcggtc atgacctata
     1141 acaaacattt ttgcaaactg atacctgatc aggtacaagc ctgttgatta ttatgctgaa
     1201 tatttcatgt tataaaacca tcga
//
LOCUS       DAZKGF010000411         1212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZKGF010000411 DAZKGF010000000
VERSION     DAZKGF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.414"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1001)
                     /locus_tag="ACHOQV_005258"
     CDS             complement(<1..1001)
                     /locus_tag="ACHOQV_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP9633460.1"
                     /translation="MRSFLTSKADSIVQQLSGLSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQTPVSDGTPALPLRTVFGVVESFRRIS
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPSRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYESGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTAGDAESPETGAYFARLRHERILNAQYCVSGHSSSPYLTPGQVLEADG
                     "
     gene            complement(986..>1212)
                     /locus_tag="ACHOQV_005259"
     CDS             complement(986..>1212)
                     /locus_tag="ACHOQV_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633461.1"
                     /translation="YDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSHMAAKQKP
                     QALTLAWSDEDGIVLVFDAQAEGESSCAVS"
BASE COUNT          274 a          364 c          315 g          259 t
ORIGIN      
        1 ccgtccgctt caagtacctg acccggcgtc agataaggac tggaggaatg cccgctgaca
       61 caatactgcg cgttcagaat gcgttcgtga cgcagacggg caaagtatgc cccggtctcc
      121 gggctttcag cgtcacctgc cgtcaggaag ggttcagcat aatggtacgt ttctccggtg
      181 gtgatccctt ccttactgaa aatactttcg gcactgtcct gaggtttcag cgcttcacgg
      241 tagttgtagt cccgggtgac cacacttccg ctcaccacgt tacaggtggt ctgaatatcc
      301 cagatgctct cctgtccgct gtcactcatc ccggcctgat tgcgcaatgg cagcgtgatg
      361 ccagattcat actgctgctg gctgtcctgg aaaatcacta tgtcctgctc aagacggctg
      421 tccatttcat aacgccagta aatccccacc tccgccagca ggcgccggat aaactcaagg
      481 tcagtttccc gccaccgggt gatgagctcc cgggatggat actcccggga gagccggaac
      541 tcaaaatcct gcccttccag accatgttta cgcaatacct gctcaaccac ttcagtgact
      601 gactgattga ggtagatttc gctccctttc gtatagttca gaagggcaat acggggaacg
      661 atcgtcagtg cataacgggt ctcatcattc gaggcagaaa tgcgacgaaa cgactcaacc
      721 acgccgaata ccgttctgag cggcagggcc ggtgttccgt cagacaccgg ggtctggaaa
      781 gtaaatgacg ccggcttcag cagtaacgcg tcacaggcaa tatccgcaga agagcaggtg
      841 acaaccacgt catagcgcca gggctggctg agggactcaa ccgcgctgta acgcaacaca
      901 tccagaaaat ggctgcattc atgcacatga acccggtacc gtgatagccc tgaaagctgc
      961 tgtacaatgc tgtcagcctt gctggtcagg aaactgcgca tgacgactct ccctctgcct
     1021 gtgcatcaaa taccagaaca atgccatcct cgtcactcca tgccagagtc agcgcctgcg
     1081 gtttctgctt cgcggccata tggctcagca actgccggct cagtaccggc agcagttgct
     1141 ggttcagcag actctccaca ttccgggcgc ccgtatccgg cagcaggcag gcggatgtca
     1201 gggcatcgta ca
//
LOCUS       DAZKGF010000412         1212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZKGF010000412 DAZKGF010000000
VERSION     DAZKGF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.415"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1001)
                     /locus_tag="ACHOQV_005260"
     CDS             complement(<1..1001)
                     /locus_tag="ACHOQV_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP9633462.1"
                     /translation="MNRSLTSKAGSVVQQLSGQSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQVPMSDGTPALPIRTVFGVVESFRRIF
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPPRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYEFGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTTGDAESPETGAYFARLRHERILNAQYCVSGHSSSPYLTPGQVLETDG
                     "
     gene            complement(998..>1212)
                     /locus_tag="ACHOQV_005261"
     CDS             complement(998..>1212)
                     /locus_tag="ACHOQV_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633463.1"
                     /translation="YDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSHMAAKQKP
                     QALTLTWSDEDGIVMVFDAQAEGEPS"
BASE COUNT          271 a          360 c          312 g          269 t
ORIGIN      
        1 ccgtccgttt caagtacctg acccggcgtc agataagggc tggaggaatg cccactgaca
       61 caatactgcg cgttcagaat gcgttcgtga cgcagacggg caaagtatgc cccggtctcc
      121 gggctttcag cgtcacctgt cgtcaggaag ggttcagcat aatggtacgt ttctccggtg
      181 gtgatccctt ccttactgaa aatactttcg gcactgtcct gaggtttcag cgcttcacgg
      241 tagttgtagt cccgggtgac cacacttccg ctcaccacgt tacaggtggt ctgaatatcc
      301 cagatgctct cctgcccgct gtcactcatc ccggcctgat tgcgcaatgg cagcgtgatg
      361 ccaaattcat actgctgctg gctgtcctgg aaaatcacta tgtcctgttc aagacggctg
      421 tccatttcat agcgccagta aatccccacc tccgccagca ggcgccggat aaactcaagg
      481 tcagtttccc gccaccgggt gatgagttcc ctaggtggat actcccggga gagccggaat
      541 tcaaaatcct gcccttccag accatgttta cgtaatacct gctcaaccac ttcagtgact
      601 gactgattga ggtagatttc gctccctttc gtatagttca gaagggcgat acggggaaca
      661 atcgtcagtg cgtaacgggt ctcatcattc gaggcaaaga tacgacgaaa cgactcaacc
      721 acgccgaata ccgtcctgat cggcagggcc ggtgttccgt cagacatcgg gacctggaaa
      781 gtaaatgacg ccggcttcag cagtaacgcg tcacaggcaa tatccgcaga agagcaggtg
      841 acaaccacgt catagcgcca gggctggctg agggactcaa ccgcgctgta acgcaacaca
      901 tccagaaaat ggctgcattc atgcacatga acccggtacc gtgattgccc tgaaagctgc
      961 tgtacaacgc tgccagcctt gctggtcagg gaacggttca tgacggctct ccctctgcct
     1021 gtgcatcaaa taccatcaca ataccgtcct cgtcactcca tgtcagagtc agcgcctgtg
     1081 gtttctgctt cgcggccata tggctcagca actgccggct cagtaccggc agcagttgct
     1141 ggttcagcag actctccaca ttccgggcgc ccgtatccgg cagcaggcag gctgatgtca
     1201 gggcatcgta ca
//
LOCUS       DAZKGF010000413         1211 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZKGF010000413 DAZKGF010000000
VERSION     DAZKGF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.416"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            130..732
                     /gene="ais"
                     /locus_tag="ACHOQV_005262"
     CDS             130..732
                     /gene="ais"
                     /locus_tag="ACHOQV_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFP9633464.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(771..1196)
                     /gene="nudI"
                     /locus_tag="ACHOQV_005263"
     CDS             complement(771..1196)
                     /gene="nudI"
                     /locus_tag="ACHOQV_005263"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP9633465.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
BASE COUNT          316 a          279 c          264 g          352 t
ORIGIN      
        1 gcaaaatttt gaggcatttt gccggcatcg taggattttt aatattacac caactgctaa
       61 ttatcgccag aaatgtcgct caatttcacg gtaattgtct ggttgcgctt gtctataggt
      121 ggagtttacg tgttagcttt ttgccgctct tcgttgaagt caaaaaaata tttcatcatt
      181 ttactggcgc tcgctgcaat tgccggactg ggtactcatg ccgcctggag tagcaatggt
      241 ttgccacgta tcgacaataa aacgctggcc agactggcac agcagcatcc ggttgtcgtt
      301 ttgtttcgcc atgctgaacg ttgcgatcgt tcaaccaatc aatgcttgtc agataaaaca
      361 ggtattacgg tcaaaggtac ccaggatgcc cgtgaactgg gcaacgcttt tagtgctgat
      421 atccctgatt tcgatctcta ttccagtaat accgtccgga ccattcagtc ggcgacctgg
      481 ttttcagcgg gtaaaaagct gacggtagat aaacgacttc ttcaatgcgg taatgagatt
      541 tatagtgcaa ttaaagactt acaacgcaaa gcgcctgata aaaatatcgt tatttttacc
      601 cataatcatt gcctgacata tattgctaaa gataagcgtg atgcgacatt taaacctgat
      661 tatctggatg gtttagtcat gcatgtggaa aaaggcaaag tttatctgga tggggaattc
      721 gttaaccact aaattttcaa tctgacagcc agtgatggct gtcatcatta ttacagaaga
      781 cctttcaaac gtaacgtttt tcgggtggcg acattcaaat catagtgcgc cagatcttca
      841 ggttttaccc acgcgtagtc ttgaaactct tcgtttattt tcacttctcg gttggcagaa
      901 acgcagtcaa aaatcaggta aatcatataa atctcttcct tgcgaccatc tgcatacgtc
      961 ttggtgcgaa tatcatcgct gaaggtccac ggcgtgattt ctgtcaaaag cagctgttct
     1021 cccagctctt cgcgaatttc gcggcgtagt gcctcttcaa ttcgttcgcc gggctccacg
     1081 ccgccacccg aaagcgccca ttgaccgggg aaaacgccgc gatcgtcggc cattttacac
     1141 agcaaataag caccatcatt ttgaatcaaa gggcatacaa tagtccgttg tcgcacaggg
     1201 gtattcctta t
//
LOCUS       DAZKGF010000414         1203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZKGF010000414 DAZKGF010000000
VERSION     DAZKGF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.417"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            332..652
                     /locus_tag="ACHOQV_005264"
                     /pseudo
     CDS             332..652
                     /locus_tag="ACHOQV_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            662..853
                     /locus_tag="ACHOQV_005265"
     CDS             662..853
                     /locus_tag="ACHOQV_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633466.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFSAL
                     KKENVGEFALRGVCVPGGE"
BASE COUNT          338 a          326 c          274 g          265 t
ORIGIN      
        1 caaatggcac attttcagta agattctgaa tgttacattc ccccttcatg tgtgacacag
       61 ctgtcacaga gaacgaaacg gaaagcacat gaaaaacata aaaactcaca ccggactcct
      121 tatccacaaa gaacaaacac ggcgcgttcg tcttcatgaa acaccggccg cctggtgcca
      181 cacacgccgg gaatgctaca gcaaaacgac cggtcgtcgc tgcggcagcc ctgacagtct
      241 cagcaggctg attctgagca gcattcagcc catagaatga gataacccgt atgaatgatg
      301 acagacgtct acggtacccg atgtatggaa catttgcttt ctggctaacg gcatgcccga
      361 cacatggttt tacaccgatg acgcctccgt cacaaaagag tgggggcggg aatattgtga
      421 caatgtggaa gtgttccctc tgtgctatat gcatgacggt cccgttttcc gggggcgcta
      481 tattgatgag ctcagcgccg cacggaacag aatgctccat atgcaccaca tactgccgga
      541 atgaacccgg tacagagaaa attttcatga cgcagaatgt atccgtgaaa ttcacgaagc
      601 gcccctgcag gctcccggca ttctgcaaaa atacaaacgc acaaaatcat aagactgttc
      661 tatgtggata ctgattttct ggctctcatc cccctttaat gtctcctccg gcgatgcccg
      721 gcaacccaca ctggccgttc acagtcagga gttccgtact gaaaaagcct gtcgggatgc
      781 cttcagcgcg ctgaaaaaag aaaacgtggg agaattcgct ctccggggcg tctgcgtccc
      841 cggcggagaa taaaccggag atgatgattc tccacaggag tattcaatgg cataccattc
      901 cccggatgtc acactgcatt atccgtatct gcgccggatg tcctgacacg tctgcctgaa
      961 tggccggaag agcgcttgca cgacttacta ccgttctcag cctaccgatt cgcagaatta
     1021 actgacgtta cggtcatacc acctaatgca gggaaacgag tcatggaggt cgtcagctct
     1081 actcccatga atgacacagc ctctgaagta aaaataccga cgcagtgaaa ccagccttaa
     1141 gctttccaaa acacaattac aacatagttg aacaaagttc attcaaacag gttcttttaa
     1201 taa
//
LOCUS       DAZKGF010000415         1188 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZKGF010000415 DAZKGF010000000
VERSION     DAZKGF010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.418"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            52..486
                     /locus_tag="ACHOQV_005266"
     CDS             52..486
                     /locus_tag="ACHOQV_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HFP9633467.1"
                     /translation="MNDNALVNVDDAVVARIRDVLAYTFPGQKFSVRKCDPSFPKVFN
                     NVWVSWLDGPTEAEVRSVTEGFENKDGLLSVLESRYFSDELVQECIDRLRKEYGQGNV
                     PLSVTVERYRKNELEKIETARFPGNISVAINEIGINTSKYRR"
     gene            490..804
                     /locus_tag="ACHOQV_005267"
     CDS             490..804
                     /locus_tag="ACHOQV_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633468.1"
                     /translation="MAGKCLVWFCNGISEPCLIAGNISELLADVTITSNYRGDVTSEI
                     RDVVQEWLRTGRGNLADLKEELWYYNLFINPSADELMNVNRRYGPERTAWLQGFIRNN
                     GA"
     gene            888..>1188
                     /locus_tag="ACHOQV_005268"
     CDS             888..>1188
                     /locus_tag="ACHOQV_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP9633469.1"
                     /translation="MSEWRKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRHGTGLSSPANQAFEQWLGLLVKNAVLDGETIK"
BASE COUNT          304 a          252 c          344 g          288 t
ORIGIN      
        1 cctcacgggg cagtgctctc tgttatttca ggattacaga ggaactgagt gatgaacgat
       61 aacgcactgg ttaatgttga cgatgctgtt gtggcccgta tcagggatgt gctggcgtat
      121 acatttccgg gacagaaatt ttctgtgcgg aaatgcgatc catctttccc gaaagttttc
      181 aacaatgtgt gggtgagctg gctggatggt ccaacggagg ccgaagtacg ctctgtcaca
      241 gaggggtttg agaataaaga cgggctgctg tctgtgcttg aatcccgtta tttcagcgat
      301 gaactggtac aggagtgtat tgatcgcctt cgtaaagagt acggacaggg gaacgtcccg
      361 ttgagtgtga ccgtggagcg ttaccggaaa aacgagctgg agaagattga aacagcgcgt
      421 tttccgggca acatatccgt agccataaac gagatcggga taaataccag caaatacagg
      481 aggtaaggta tggctggtaa atgccttgtc tggttctgta acgggatctc tgaaccgtgt
      541 ctgatagcag gaaatatcag tgaattactg gcagatgtta cgatcacgtc aaattaccgt
      601 ggagatgtca catctgaaat cagggatgtt gtacaggagt ggctcaggac cggcaggggg
      661 aacctggcgg atttaaagga agagctctgg tactacaacc ttttcatcaa tccgtctgca
      721 gacgaactga tgaatgtcaa caggcggtat ggccctgagc gaactgcctg gctgcagggt
      781 tttatccgca acaacggggc gtgatacccc gttttcccgt gagggcattg tacctcacgg
      841 gacaatgccc tctttttaca tctgtataaa aaagaggagc gtaaacaatg tctgaatggc
      901 gcaaaaaccg ttttgaaatt accggtaaat cagtctgtct tgatgtactt acacagtgga
      961 ttgaagggcg tgagaccccg cgctaccgtc atgccataca gcagagtata cagctgtttc
     1021 tggccggttg tgcagggctg gtaaaaccca cacggacgac gcagtatccg ccatatccgg
     1081 cgctggttcg tcacggaacg gggttgtcat ctccggctaa ccaggcgttt gagcagtggc
     1141 tggggttgct ggtaaaaaat gccgttctgg atggtgaaac gataaaga
//
LOCUS       DAZKGF010000416         1185 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZKGF010000416 DAZKGF010000000
VERSION     DAZKGF010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.419"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1185)
                     /gene="tssF"
                     /locus_tag="ACHOQV_005269"
     CDS             complement(<1..>1185)
                     /gene="tssF"
                     /locus_tag="ACHOQV_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP9633470.1"
                     /translation="DARLHTESDGRSAIRLRFECPEKVDWSKAGIDKVAIFLNAEAPV
                     SAALHLAMTRRVHAMYARHAGTYTGRHQFDGWCRPMGFDDNDCLWKKADTAFSGYQLL
                     LEYFSFRPKFMFVELRGLDTIGLTAASTWFEIDIVLSEAWSSCLPFETENFRLHCAPV
                     INLFTLEADPLLLNPLDNEYLLRPLRLQDGHTEIYSVDNIHGSVKNGRHPYVPFTSFR
                     HRGGMMRHDAPERYYHTRVKRGPSGLYDTWLVLGGRSFELEQLSDKPESLSMRITATN
                     GQLPRRALESTLLDRVVKTGKVPVRVLNLTAPTMPLYPPANDRFHWRVMSHLGSNFLS
                     MMDNPEVLRGTLALYDWTDDEMNRRRLEAIVAVKHTLIRRFEKGFMLRGVDIEVTLNM
                     DNF"
BASE COUNT          270 a          326 c          338 g          251 t
ORIGIN      
        1 aaaattatcc atattcagcg tgacctcaat gtccacgcca cggagcataa agcccttttc
       61 aaaacggcgg atcagggtgt gcttcacggc aacgatggct tcaagccggc ggcggttcat
      121 ttcatcgtcg gtccagtcat agagcgccag cgtcccccgc agcacctccg gattgtccat
      181 catgctgaga aagtttgaac cgaggtgact catcacccgc cagtgaaacc ggtcatttgc
      241 cgggggatac agtggcatcg tcggtgcggt caggttcagg actctgacgg ggactttgcc
      301 ggttttgacc accctgtcca ggagcgtact ttccagcgcc ctgcgcggaa gctgcccgtt
      361 ggtcgcggtg attcgcatcg agagggactc cggtttatca gacaactgct ccagttcgaa
      421 cgaacgtccc ccgaggacta accatgtgtc atacaggccg gagggtcccc gtttcacgcg
      481 ggtgtggtaa taacgttcag gagcatcgtg gcgcatcata ccgccccggt gccggaagct
      541 ggtgaacggg acataagggt gtctgccatt cttcaccgag ccgtgaatgt tatcgacgct
      601 gtaaatttcg gtgtgaccat cctgaagacg cagcggcctg agcaggtact cgttatccag
      661 cgggttaagg agcagcgggt cggcttccag ggtaaagaga ttgataaccg gcgcacaatg
      721 caggcggaag ttttctgtct caaaaggcaa acaggatgac caggcttcac tgagcacaat
      781 gtcgatttca aaccaggtac tggccgcagt gagcccgatg gtgtcgaggc cgcgcagttc
      841 aacaaacata aacttcggac ggaagctgaa atactccagc agcaactggt aaccactgaa
      901 ggctgtatcg gctttcttcc acaggcagtc attgtcgtca aagcccatgg gtctgcacca
      961 gccgtcaaac tgatgacgtc cggtatatgt cccggcatga cgcgcataca tggcatgtac
     1021 acgccgggtc attgccagat gcagagcagc gcttaccggc gcctctgcat taagaaaaat
     1081 ggcgactttg tcgatcccgg ctttactcca gtccactttt tcagggcatt cgaagcgcag
     1141 gcggatggca gagcggccat cggattcagt atggagacgg gcatc
//
LOCUS       DAZKGF010000417         1167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZKGF010000417 DAZKGF010000000
VERSION     DAZKGF010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.420"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            240..485
                     /locus_tag="ACHOQV_005270"
     CDS             240..485
                     /locus_tag="ACHOQV_005270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633471.1"
                     /translation="MEEMQDKMAWMQNVITEKLGPSLWHMHPIAFPGALIKKNTGWAH
                     SKFANLLGSVELENDYSAYNVTKTGKSVPHYNINLTK"
     gene            656..>1167
                     /locus_tag="ACHOQV_005271"
     CDS             656..>1167
                     /locus_tag="ACHOQV_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633472.1"
                     /translation="MGCNKKSNKFLLFVSCMLLSFVTYSQDSSNPQFSDYLVPVSNGP
                     FEKNIYFNKEQENYSQHWKNAVQEELKKPVNFAGHFRIYTAFGGHGKECLRDNWVCGW
                     VIDKLSGEVVATLPSDNNGSYNYADVSDNGTPVGLPFEIDTYKNSSMIAITGQSISVS
                     KSDNPVCKTT"
BASE COUNT          398 a          179 c          242 g          348 t
ORIGIN      
        1 gactctaaaa attctgagtg aacaacaaat tagcgaagtt aaaatagttg cgtttcttat
       61 ctggtcggtt tgatattttt gttagtattg ccatacttgt aaagttaatc aaatatcatc
      121 aattgattta gtgtaatttc aaaatattaa tatatcaaaa tacgatgagt taaacgactt
      181 tataacgaat tcaaaactaa tgaaaaatgc ttaaatgcac tgaaaaaaga cgtaccggaa
      241 tggaagaaat gcaggataag atggcctgga tgcagaatgt gatcacggag aaactgggcc
      301 cttcgttgtg gcatatgcat ccgattgcct ttcctggagc attaataaag aaaaatactg
      361 ggtgggcgca tagcaagttt gcaaaccttt tgggaagtgt cgagttagaa aatgattatt
      421 cggcatataa tgttactaag acaggtaaat ctgtcccgca ctataatatt aatctcacaa
      481 aatgacatta tctgaggtaa ttgatgcccc aagagaacga aaaatggtaa aaagaaatac
      541 aggttagtac ctagggtggc agaagttgag ggggagtcgt actattctgg tgatggattg
      601 aataaagctc acctattacc agatacgatg attaaagctc ttgaggattc taaaaatggg
      661 atgtaataaa aaatcaaaca aatttttgct ttttgtgtca tgcatgctct tgtcgtttgt
      721 aacgtactca caagacagtt ctaatcctca gttttctgac tacctggttc ctgtaagcaa
      781 cggaccattt gaaaagaata tttactttaa taaagagcag gaaaattact cgcaacactg
      841 gaagaatgca gtccaagagg aactaaaaaa gcctgttaat tttgcagggc actttagaat
      901 ctatacagcc tttggcggtc atggtaaaga atgccttcgt gataattggg tatgtggttg
      961 ggttattgat aaactatcag gggaagttgt agcaacactt ccatcagata ataatggaag
     1021 ttataactat gctgatgtgt ctgataatgg tacccctgtt ggtcttccat ttgagattga
     1081 tacttataaa aatagttcaa tgattgcaat aaccggtcaa tctatctcag taagcaagag
     1141 tgataatcct gtatgtaaaa cgacttt
//
LOCUS       DAZKGF010000418         1142 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZKGF010000418 DAZKGF010000000
VERSION     DAZKGF010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.421"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            20..742
                     /gene="sdiA"
                     /locus_tag="ACHOQV_005272"
     CDS             20..742
                     /gene="sdiA"
                     /locus_tag="ACHOQV_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFP9633473.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCTAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(809..1033)
                     /gene="yecF"
                     /locus_tag="ACHOQV_005273"
     CDS             complement(809..1033)
                     /gene="yecF"
                     /locus_tag="ACHOQV_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFP9633474.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(1020..>1142)
                     /gene="yecU"
                     /locus_tag="ACHOQV_005274"
     CDS             complement(1020..>1142)
                     /gene="yecU"
                     /locus_tag="ACHOQV_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFP9633475.1"
                     /translation="ITLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
BASE COUNT          293 a          249 c          282 g          318 t
ORIGIN      
        1 aggggcgttg cggtttacta tgcaggatac ggattttttc agctggcgtc gcacgatgct
       61 gttgcgtttt cagaggatgg aggccgcaga agaggtctac catgaaatag agcttcaggc
      121 tcagcagctg gagtacgatt actattcgtt atgtgttcgc cacccggtac cattcacgcg
      181 acctaaagtg gctttctaca ccaattaccc tgaggcgtgg gttagttatt atcaggcaaa
      241 aaactttctc gcaattgatc ctgtactgaa ccctgaaaac tttagtcagg gccatttaat
      301 gtggaatgat gacttattca gcgaagcaca gccgttatgg gaagctgcgc gcgcgcatgg
      361 tttacgccgc ggtgtcactc agtatttaat gctgccaaac cgggcgctgg gctttttgtc
      421 cttttcccgt tgcactgcgc gcgaaatacc cattcttagt gatgaactgc aattaaaaat
      481 gcagttactg gtgcgcgaaa gtctgatggc tctgatgcgt ttaaatgatg aaatagtgat
      541 gacgccagag atgaatttca gcaagcgcga aaaagaaatt ctgaaatgga cggcggaagg
      601 gaagacatca gcagagatag cgatgatttt gtcaatctct gagaatacgg tcaatttcca
      661 tcagaaaaac atgcagaaaa aaatcaatgc accaaataag acccaggtgg cctgttacgc
      721 ggccgctact ggcttaattt gatcgctttt ctttctgcgt gccagatgca aaaaccggct
      781 gaaaggcacg ctctcagccg gttttatatt actgacggta ggcttgttta atttgcttaa
      841 ccgttttgga aaatactgca gcctgggttt cgtcttcgat cagcgcaagc tgtttttcca
      901 tctttaacat cacgcggccc gcgtcagctt gtcccatcgc ctgcagcatc agcgtcagca
      961 tcgctttcag gcaggagact tcatttgcca gttcttgatt attctcggca gtagaaaaat
     1021 caggcgtact catttatttt cctcgtcatg ttgcaatgaa aatttgcggt gaaaaatgtt
     1081 aaggcggcgg agtataccat aagctttgct aaaaatagca gtggttgttt tttgagcgtg
     1141 at
//
LOCUS       DAZKGF010000419         1108 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZKGF010000419 DAZKGF010000000
VERSION     DAZKGF010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.422"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1096
                     /locus_tag="ACHOQV_005275"
     CDS             <1..1096
                     /locus_tag="ACHOQV_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFP9633476.1"
                     /translation="EDVQKTGLITNTSPMSPFWRLILTIVIRPVQWLRDALVDVVLPG
                     MFVATADRSLLLLHAHDVNITPKPASKARGVIWFAKTRANDDVLIPEGTIVQTARING
                     VIYSMAVTEETLISSGKSGASVPVEATTEGSGHNLAPGYFRILPVAIPGIASVVNRED
                     WLTVPGADEESDDELRDRIRNQFNLVSSYHTDAVYRGLIASVAGLSIDRIYFQHDAPR
                     GPGTANAYLLLDSGEASAPFIEAVNRYINEEGHHGHGDDLQCFAMPETRHRLDVTVYI
                     RSSTTLPPDGAEGLKQDISDLIRCAFRENARFNVKKVWPYSRFSFSTLGKEIHQTFPV
                     VASVQFSLEDIISDLAVPRLESLTVEVVIE"
BASE COUNT          245 a          330 c          308 g          225 t
ORIGIN      
        1 tgaggatgta cagaaaaccg gactcatcac caacacctcg cccatgagtc ccttctggcg
       61 gctcattctg accattgtca tacgacccgt tcagtggctg cgtgatgcgc tggttgacgt
      121 tgtgctgccc ggtatgtttg tcgccacggc tgaccggtca cttcttctgc ttcatgcgca
      181 cgacgtcaac atcaccccaa aaccggccag taaggcccgt ggcgtgatct ggtttgccaa
      241 aaccagggcg aatgatgatg ttctcatccc cgaagggacc atcgttcaga cagccagaat
      301 caacggcgtg atatacagca tggccgtcac ggaggaaacg cttatcagca gcggtaaaag
      361 cggcgcatcc gttcccgtgg aagccaccac cgaaggcagc gggcacaacc tcgcgccggg
      421 gtattttcgt atccttcccg ttgccattcc cggcattgca tccgtggtga accgggagga
      481 ctggctgacc gtaccgggtg ccgatgagga aagcgacgac gaactgcgcg acagaatacg
      541 caaccagttt aacctcgtga gcagttacca cacggacgcc gtctaccggg gacttatcgc
      601 cagcgtggca ggcctgagca tcgaccggat ctactttcag cacgacgccc cgagaggacc
      661 gggaacggcc aacgcctacc tgttactgga cagcggcgag gcctctgccc ctttcattga
      721 agcagtcaac cgctacatca acgaagaggg gcaccacgga cacggggatg atttgcagtg
      781 tttcgccatg ccggagacgc gtcaccgtct ggacgtcacc gtatacatca gaagctcaac
      841 caccctgcca ccggacggcg cggaagggct gaaacaggat atcagcgacc tgatacgctg
      901 cgcttttcgg gagaacgccc gctttaatgt gaaaaaagtc tggccctatt cgcgcttttc
      961 gttttccacg ctgggcaagg aaattcatca gaccttcccg gtggtggctt cggttcagtt
     1021 ttcactggaa gacatcatca gcgatctggc cgtgccacgg ctcgaatccc tgacagtgga
     1081 ggtggtcatt gaatgacttt ttgcagcg
//
LOCUS       DAZKGF010000420         1104 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZKGF010000420 DAZKGF010000000
VERSION     DAZKGF010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.423"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1104
                     /locus_tag="ACHOQV_005276"
     CDS             <1..>1104
                     /locus_tag="ACHOQV_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFP9633477.1"
                     /translation="AERLRSQLVLQQAIARLQHREATGAPWYTRFGLNQDSDTLAALW
                     PLYAKNNAQLMRDATADSLRQQLNAFVQLPPASDARTQGTQHAYDILKSYLMLARPDK
                     ADAGWLAKNALKALPKRRNVPDGIWQNVAPKLLGFYAQNLPAHPEWKIKPDDELISTV
                     RQILLKQIGQRNAESGLYQEMLKRVASNWPDLTLADMTGDTDASTVFSTDEVVPGMFT
                     RQAWEEQVQDAINEVVRTRRDEIDWVLTDKTHQTGSDISPEALKSRLTERYFTDFGNA
                     WLNMLNSIQWHEPTSLSGAIAQLSLLGDVRQSPLVALMNTLNYQGKTGQKGEALADSV
                     VDSAKKLLDKNKNGRQFIEQTQGPEGPLDGVFGP"
BASE COUNT          283 a          285 c          313 g          223 t
ORIGIN      
        1 ggctgaacgc ctgcgcagcc agcttgtact ccagcaggcc attgcccgtc tgcaacatcg
       61 tgaggcaacc ggtgcgccct ggtacacacg atttggtctt aatcaggaca gcgacacgct
      121 ggccgcgctc tggccgctgt atgcgaaaaa taacgcacag ctgatgaggg atgccacagc
      181 agacagcctc aggcagcagc tgaatgcctt tgtgcaactc cctcctgcca gtgatgcccg
      241 tacgcagggc acacagcatg cttacgacat actcaaaagt tatctgatgc tggcgcgccc
      301 ggacaaagcg gacgccggct ggctggcaaa aaatgcgctg aaagccttac cgaaacgccg
      361 gaatgtgccg gatggcatat ggcagaacgt cgcaccgaaa ctgctggggt tttatgcgca
      421 aaaccttccg gctcatccgg aatggaaaat taagcctgac gatgaattaa tcagtacggt
      481 ccgtcagatt ctgcttaagc aaattggcca gcggaatgct gagtccgggt tatatcagga
      541 gatgcttaag cgcgtggcca gtaactggcc tgatttaaca cttgcggata tgaccggtga
      601 caccgatgcc tcaacggtat tcagcactga cgaagtcgtt ccgggaatgt ttacgcgtca
      661 ggcctgggaa gagcaggtac aggatgcgat taacgaagtt gtcagaacac gccgcgatga
      721 aattgactgg gtactgacgg ataaaacaca tcagacaggg agcgatatct ctccggaggc
      781 cctgaagagc cgcctgactg aacgctattt taccgatttt ggtaatgcct ggctgaacat
      841 gctcaacagc atacagtggc atgaacccac gtcactttcc ggagccattg cccagttgag
      901 tctgctcggt gacgtccgcc agtctccgtt agttgcactg atgaacacgc tgaactacca
      961 ggggaaaacg gggcagaagg gggaggcact ggctgattca gtcgtggatt cagcgaaaaa
     1021 gctgctcgat aagaataaaa atggcaggca gtttattgaa cagacacagg gccctgaagg
     1081 tccgctcgat ggggtatttg gacc
//
LOCUS       DAZKGF010000421         1100 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZKGF010000421 DAZKGF010000000
VERSION     DAZKGF010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.424"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..1069
                     /gene="ybhH"
                     /locus_tag="ACHOQV_005277"
     CDS             17..1069
                     /gene="ybhH"
                     /locus_tag="ACHOQV_005277"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFP9633478.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLASIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISCSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
BASE COUNT          282 a          249 c          279 g          290 t
ORIGIN      
        1 aattgagggc attattatga aaaaaatacc ctgcgtgatg atgcgaggtg gaacctcgag
       61 gggcgcgttc ctgttagcgg aacatctacc cgaagatcaa acgcagcgcg ataaaatatt
      121 gatggcaatt atgggttccg gtaacgatct ggaaattgac ggtattggcg gcggtaatcc
      181 gctgaccagt aaagtcgcca ttattagccg ctccagcgat ccgcgtgcag atgtcgatta
      241 tctgtttgct caagttatcg tccatgagca acgtgtcgat accacgccta actgcggcaa
      301 tatgctgtct ggcgttgggg cattcgccat tgaaaatggt ttgattgcag cgacttcgcc
      361 agttacccgc gtacgtatcc gcaacgtcaa tacgggtacg tttatcgaag ctgatgtgca
      421 aacgccaaat ggtgttgtcg agtacgaggg tagcgccaga attgacggcg taccgggtac
      481 tgccgcaccg gttgcgctca ctttcctgaa tgccgccgga acgaaaaccg gaaaagtctt
      541 cccgacggat aatcagattg attattttga cgatgtcccg gtgacctgta tcgatatggc
      601 gatgccagtt gtcattattc ctgctgaata tctgggaaaa acaggctatg aattaccggc
      661 agagctggat gccgacaaag cattattagc cagcattgaa tctatccgtc tacaagcggg
      721 taaagcaatg ggcttaggcg atgtcagtaa tatggttatc cctaaacctg tacttatttc
      781 tccagcgcag aaaggcgggg caattaatgt gcgttatttt atgccgcatt cttgccatcg
      841 cgctttggcg ataaccggtg ctattgctat ttcctgtagt tgtgcattgg aaggcaccgt
      901 cacccgacaa atcgtccctt ctgtaggata cggcaatatc aatattgaac accccagtgg
      961 tgcgctcgac gttcatttaa gtaatgaagg tcaggatgcc acgacgttac gcgcatctgt
     1021 tattcggacg acaagaaaaa tattttccgg tgaagtttat cttccctgaa aaattttgtt
     1081 gtcgtgataa tgacaatcaa
//
LOCUS       DAZKGF010000422         1091 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZKGF010000422 DAZKGF010000000
VERSION     DAZKGF010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.425"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..594
                     /locus_tag="ACHOQV_005278"
     CDS             <1..594
                     /locus_tag="ACHOQV_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9633479.1"
                     /translation="YYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMG
                     LASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRAL
                     SPYDYTLYCRKWVKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEP
                     YLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            591..>1091
                     /locus_tag="ACHOQV_005279"
     CDS             591..>1091
                     /locus_tag="ACHOQV_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633480.1"
                     /translation="MKRILFFLLMIFVSFGVIADCETQAKDQDCFTIFTKGTIFSAFP
                     VLNNKTIWRWYQNEDIGEYYWQTELGICKNNKFTPSGARLLIRVGSLRLNENHATKGT
                     LQELLNTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLP"
BASE COUNT          362 a          216 c          233 g          280 t
ORIGIN      
        1 tattacaacc gccaccgcta ttatgacccg ctgcaggggc ggtatatcac tcaggatcct
       61 attgggctga aggggggatg gaatttttat cagtatccgt tgaatccggt tcagtatata
      121 gattcaatgg gactggcatc aaaatatgga cacttaaata atggcggata tggagcgaga
      181 cccaacaaac cgcctacgcc cgatccaagt aaattgccag acatagcgaa acaattaaga
      241 ctgccatatc ctattgacca ggccagtagt gcgcctaatg ttttcaaaac attcttcaga
      301 gcattaagcc cttacgacta cacactgtat tgcaggaagt gggtaaaacc aaatctgact
      361 tgtacgccac aggatgatcc ccagtatcca gggatggata caaagacagc aagtgattac
      421 ctgccacaga caaattggcc aacaactcaa ttaccaccag gatatacttg tgcagaaccc
      481 tatttattcc cagacattaa taaacccgat gggccagcaa cagcagggat agatgatttg
      541 ggtgaaattt tagctaaaat gaaacagaga acatcgagag gaataagaaa atgaaaagaa
      601 ttttgttctt tttgctgatg atatttgtta gttttggtgt tatagctgat tgcgaaacac
      661 aagctaaaga tcaagattgc tttaccattt tcactaaggg tactattttt tccgcgtttc
      721 ctgtcttaaa taataaaacc atatggagat ggtatcagaa tgaggacata ggtgagtatt
      781 attggcaaac agaacttggt atatgtaaaa ataacaaatt cacaccaagc ggggcgagac
      841 tcttaattcg cgtcggatca ctacgcctta atgaaaatca tgcaaccaaa ggaacgttgc
      901 aggagttgct aaatacagca gaaaaaacgg cgtttctcgg cgatcggttt agaagttata
      961 taagagcggg tatctatcaa aaaaagagca gtgatccagc tcagttgttg gcagtccttg
     1021 ataattcaat catggttaaa tattttaaag atgagaagcc aacttatgct cgaatgacag
     1081 cacatctccc g
//
LOCUS       DAZKGF010000423         1079 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZKGF010000423 DAZKGF010000000
VERSION     DAZKGF010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.426"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            15..>1079
                     /locus_tag="ACHOQV_005280"
     CDS             15..>1079
                     /locus_tag="ACHOQV_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9633481.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIIN"
BASE COUNT          361 a          163 c          241 g          314 t
ORIGIN      
        1 tagcaggtgt caccatgaaa aaagccatag catatatgcg attttcatca ccaggtcaga
       61 tgtctggcga ctcattaaac cgacagagaa gacttattgc tgaatggtta aaggtaaata
      121 gtgattatta tcttgatacc ataacatatg aagatttagg attaagtgca ttcaaaggaa
      181 agcatgcaca atcaggagct ttttcggaat ttttagatgc tatagagcat ggttatatat
      241 tgccaggaac tacattgtta gttgaaagtc tggacagact ttcaagagaa aaagtcggtg
      301 aagcgattga acgtctgaaa ttgattttga atcacggtat tgatgttata actctttgcg
      361 ataatacagt ctataatatt gactctttga atgagccata ttcattaata aaagccatac
      421 ttatagcaca aagggcaaat gaagaaagcg agataaagtc aagtcgggtt aaattatcat
      481 ggaagaaaaa acggcaggat gcactggaat caggtacgat tatgacggcg tcttgtccga
      541 gatggctctc cttagatgac aaaagaacgg cttttgttcc agaccccgac agggtgaaaa
      601 ctattgagct aatttttaaa ctcaggatgg aaaggcgctc attgaatgca atagccaagt
      661 atttaaatga tcatgctgta aagaatttct caggaaaaga aagtgcatgg ggaccttctg
      721 taattgaaaa attattagcg aataaagctc tgataggtat atgcgtacct tcatatcgtg
      781 caagagggaa agggataagt gaaatcgctg gctattatcc cagagtcata tcagatgatt
      841 tgttttacgc tgtacaggaa attcggttgg caccttttgg tattagcaat agtagcaaga
      901 atcctatgct aataaatcta cttcgaacag ttatgaagtg tgaggcttgt ggtaatacca
      961 tgattgttca tgcggtatct ggaagtttgc atggctatta tgtttgtccg atgagaagat
     1021 tacatcgatg tgacaggcca tcaataaaaa gagatttggt tgattataat atcattaat
//
LOCUS       DAZKGF010000424         1076 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZKGF010000424 DAZKGF010000000
VERSION     DAZKGF010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.427"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1076
                     /locus_tag="ACHOQV_005281"
     CDS             <1..>1076
                     /locus_tag="ACHOQV_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9633482.1"
                     /translation="PDPEQVPGAVMRLSCATWWNSQLRHLRDIWREELLRAAGLVSRK
                     TSVYISHEALLDWREKRQRTRDFLKAWDIENEEGERFSLEDIYWAGLSNPRNRRNEMM
                     ACVRGMEQVAEARGDVAFFVTVTAPSRFHSVNDDGSLNPKYCGATVRDASDYLVYRFF
                     AAVRKAINKAGLGWYGVRTVEPHHDGTPHWHMLVFTSPENEARITEIMRNAAIREDRA
                     ELGDDISPRFKCEKIDPAKGTPASYIATYIGKNLDASAFMGNDPKTGKPYVDKESGKT
                     MAETVENAIGWSALHRIRQFQFFGIPPRQVWRELRRLAGQMARNPAAPQRLDHDDIDA
                     ILAAADVGCFATYITRQGGVLIPR"
BASE COUNT          242 a          331 c          309 g          194 t
ORIGIN      
        1 ccccgacccg gaacaggtgc cgggtgcggt catgcgcctt tcctgtgcga catggtggaa
       61 cagtcagtta agacacctca gggacatctg gcgtgaagag ctgttacgcg ccgcaggtct
      121 ggtgtcacgt aagacatcgg tatatatcag ccatgaagcg ttactggact ggcgcgaaaa
      181 acgccagcgc acccgcgatt ttctgaaggc ctgggacatc gagaacgaag agggagaacg
      241 gttcagcctt gaggatatct actgggcagg tctctctaat ccgcgtaacc gccgtaacga
      301 gatgatggcc tgcgtccggg gaatggagca ggtcgccgaa gcacgcggcg acgtggcgtt
      361 tttcgtcacc gtcacagccc cttcccgttt tcacagcgtg aacgatgacg gcagcctgaa
      421 cccgaaatac tgcggagcaa cggtacgcga tgccagtgat tatctggttt accgcttttt
      481 cgccgccgtc cgcaaggcca tcaacaaggc cgggctgggc tggtacggcg tgcgcacagt
      541 agagccccac cacgacggta caccgcactg gcacatgctg gtattcacca gcccggaaaa
      601 cgaagcccgt atcactgaaa tcatgcgtaa cgccgccatc agggaggacc gcgcagagct
      661 gggtgatgat atctccccgc gttttaaatg cgaaaaaatc gacccggcaa aaggcacacc
      721 ggcaagctat atcgcgacct atatcggtaa gaacctggac gccagcgcct tcatggggaa
      781 tgacccgaaa accggcaaac cttacgtcga taaggaatca ggcaaaacca tggccgaaac
      841 cgtggagaac gccatcggct ggtcggccct tcaccgcatc cgccagttcc agttcttcgg
      901 tatcccgccc cgccaggtat ggcgcgaact ccgccgcctt gccgggcaga tggcccgtaa
      961 cccggctgcc ccgcagcgtc tggaccatga cgacattgac gccatactgg ccgccgccga
     1021 cgtgggatgt tttgccacct atatcacccg ccagggtggt gtgctcattc cccgca
//
LOCUS       DAZKGF010000425         1069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZKGF010000425 DAZKGF010000000
VERSION     DAZKGF010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.428"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            92..1036
                     /gene="leuO"
                     /locus_tag="ACHOQV_005282"
     CDS             92..1036
                     /gene="leuO"
                     /locus_tag="ACHOQV_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFP9633483.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          293 a          226 c          254 g          296 t
ORIGIN      
        1 attttatatg catgataaat catattctcg aggattattt ctctgcattc caataaggga
       61 aagggagtta agtgtgacag tggagttaag tatgccagag gttcaaacag atcatccaga
      121 gacggcggag ttaagcaagc cacagctacg catggtcgat ctcaacttat taaccgtttt
      181 cgatgccgtg atgcaggagc aaaacatcac ccgtgccgct catgttctgg gaatgtcgca
      241 acctgcggtc agtaacgctg ttgcacgcct gaaggtgatg tttaatgacg agctttttgt
      301 tcgttatggc cgtggtattc aaccgactgc tcgcgcattt caactttttg gctcagttcg
      361 tcaggcattg caactagtac aaaatgaatt gcctggttca ggttttgaac ccgcgagcag
      421 tgaacgtgta tttcatcttt gtgtttgcag cccgttagac agcattctga cctcgcagat
      481 ttataatcac attgagcaga ttgcgccaaa tatacatgtt atgttcaagt cttcattaaa
      541 tcagaacact gaacatcagc tgcgttatca ggaaacggag tttgtgatta gttatgaaga
      601 cttccatcgc cctgaattta ccagtgtgcc attatttaaa gatgaaatgg tgttggtagc
      661 cagcaaaaat catccaacaa ttaaaggccc gttactgaaa catgatgttt ataacgaaca
      721 acatgctgcg gtttcgctcg atcgtttcgc gtcatttagt caaacctggt atgacacggt
      781 agataagcaa gccagtatcg cgtatcaggg catggcaatg atgagcgtac ttagcgtggt
      841 gtcgcaaacg catctggtcg ctattgcgcc gcgttggctg gctgaagagt tcgctgaatc
      901 cttagagtta caggtattac cgctgccgtt aaaacaaaac agcagaacct gttatctctc
      961 ctggcatgaa gctgccgggc gcgataaagg ccatcagtgg atggaagagc aattagtctc
     1021 aatttgcaaa cgctaactga atgcagaata ggtcagacat taatgtctg
//
LOCUS       DAZKGF010000426         1058 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZKGF010000426 DAZKGF010000000
VERSION     DAZKGF010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.429"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..351
                     /locus_tag="ACHOQV_005283"
     CDS             <1..351
                     /locus_tag="ACHOQV_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9633484.1"
                     /translation="YDLNVPPEKIKIVFNQVDSSRNFDKQFAVILESEAFKELGIVNY
                     SMIPDTELFRYLSDMGKDINTVLNDDRDFRTLMRETDDPDILELLSTERAIKRLATGI
                     NKELDIAFNHLEII"
     gene            361..990
                     /locus_tag="ACHOQV_005284"
     CDS             361..990
                     /locus_tag="ACHOQV_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633485.1"
                     /translation="MSGNATNEIILAKINDILVDFNELNRQINNFPEKFSGKIDAINF
                     AIKQTPDALEESLKKLFSAAEELEKTVQESTDECRVSMRAYAINELDNLKVKISEAIN
                     ESIKNSIDMPLDQAKERIIQINKMLQKSTGVEMSTRVAFGIGGLLGLSFLSLAMCISA
                     GYLYFQERSKAERYYNAFREQQIVIERLPEETQRQFRDELGRYLARNQK"
BASE COUNT          379 a          138 c          227 g          314 t
ORIGIN      
        1 tatgatttaa atgtgcctcc agaaaaaata aaaattgttt ttaatcaggt tgattcatca
       61 cggaactttg ataaacagtt cgctgtgata ctggagagtg aagcatttaa agaactaggt
      121 atagtaaatt attcaatgat tcctgatacg gagttattta gatatctttc tgatatgggg
      181 aaagatataa acactgttct aaatgatgac agggatttta gaacattaat gagggaaact
      241 gatgatccag atatacttga gttattgtca acggaaagag ctataaagcg tcttgctaca
      301 gggataaata aagagctgga tattgcattt aatcatctcg aaattatata aggtgtaaat
      361 atgtctggaa atgcaacaaa tgaaattatt ttggcaaaaa taaacgatat tcttgtcgac
      421 tttaatgagt taaacaggca aataaataac tttcctgaaa agttttcagg gaaaatagat
      481 gcaattaatt ttgccataaa acagacacct gatgcgcttg aggaatcatt aaaaaaattg
      541 ttttctgcgg cagaagagtt agaaaaaaca gtgcaggaaa gtacagatga atgtcgggta
      601 tcaatgagag cgtatgctat aaatgagtta gataatctga aagtaaagat atcagaagct
      661 ataaatgaat caattaaaaa ttctattgat atgccgttag atcaggcgaa agaacggatt
      721 atccagataa ataagatgct acaaaaaagt accggggtag aaatgagtac aagagtagca
      781 tttggtattg gtggattgct ggggctttca tttctttctt tagcaatgtg tatatcagca
      841 ggatatttgt attttcagga gcggagcaaa gcagagcgtt attataatgc cttccgtgaa
      901 caacagatag tgatagaacg tttgccagaa gaaacgcagc gccagttccg ggatgaattg
      961 gggcgttatc ttgcccgtaa tcagaagtaa agtaggccct agttaggtgt accttaggtt
     1021 aattatacct tagttatacc ttagttatat gttaatta
//
LOCUS       DAZKGF010000427         1054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZKGF010000427 DAZKGF010000000
VERSION     DAZKGF010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.430"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1054)
                     /locus_tag="ACHOQV_005285"
     CDS             complement(<1..>1054)
                     /locus_tag="ACHOQV_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP9633486.1"
                     /translation="AVKPDVLRFRGREALSEPFRWDIGFTSRNANIPPEEVLMKYASL
                     RMRNGKNVHGIVTRLEWLSTSKDQSHYRLTLSSRLALLEYTRQCAVFQNQSVPEVVEQ
                     VLRKHGLEGADFEFRLERAYPSREIITQWQETDLQFIQRILSEVGIFWRTEMDDVRGL
                     DTYIFADSQLNYQFDVCLPYSEPSGLFDGAAESVWDVRTWHNVAIGTVSTRDYNYRTA
                     SMPMDATVSVRNDAVTTGEHYRDAPAYREAGDDTSPEPETESGAFYARLHHERELNRS
                     ARIHLFSNAAHLSPGQVLEPQGDVIADLKEGIVLTLVTYRGARDSRLHVSAWGMPYTE
                     RYCFRPAEIPRPVIPGT"
BASE COUNT          227 a          322 c          291 g          214 t
ORIGIN      
        1 gcgtacccgg gatgaccgga cgcgggattt ctgccggacg gaaacagtaa cgttcggtgt
       61 aaggcattcc ccatgctgac acatgcagcc gggaatcccg ggccccccgg taagtcacca
      121 gtgtgagaac aatcccttcc ttcaggtccg caatgacatc cccctgcggc tccagcacct
      181 gcccggggga gaggtgtgcg gcgttgctga acaggtgaat gcgggccgac ctgttgagtt
      241 cccgctcgtg gtgaagccgg gcgtaaaacg caccagattc ggtttccggc tccgggctgg
      301 tgtcatcccc cgcttcacga taagccggtg catcgcggta atgctccccg gtggtgaccg
      361 catcattacg cacgctgacc gtcgcatcca tcggcatgga ggcagtccgg tagttgtaat
      421 cacgcgtgga gaccgtgccg atggcgacgt tgtgccatgt ccgtacatcc cagaccgatt
      481 ctgcagcacc gtcaaaaagg cccgacggct cgctgtacgg cagacacaca tcaaactggt
      541 agttaagctg gctgtcggca aaaatgtacg tatccagtcc gcgcacatca tccatctccg
      601 tgcgccagaa aatccccacc tcagacagaa tgcgctgaat gaactgcaaa tccgtttcct
      661 gccactgggt gataatttca cgcgacgggt aagcgcgttc cagtctgaat tcaaaatcag
      721 cgccctccag cccgtgtttg cgcagcacct gctccaccac ctcagggacg gactggttct
      781 gaaatacggc acactgccgg gtgtactcca gcagtgccag acgggagctg agtgtaagcc
      841 ggtagtggga ctggtctttc gatgtggaca gccattccag acgggtcaca atgccatgga
      901 catttttgcc gttacgcatc cgcaacgacg catatttcat cagcacctct tccggaggaa
      961 tgttcgcatt ccgggaggtg aacccgatgt cccatcggaa cggctcactc agtgcttccc
     1021 tgccacgaaa acgtaaaaca tcaggcttta ctgc
//
LOCUS       DAZKGF010000428         1044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZKGF010000428 DAZKGF010000000
VERSION     DAZKGF010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.431"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..900)
                     /locus_tag="ACHOQV_005286"
     CDS             complement(<1..900)
                     /locus_tag="ACHOQV_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFP9633487.1"
                     /translation="MKAPVSITSFRPQKSIAIVIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFIS"
BASE COUNT          267 a          239 c          250 g          288 t
ORIGIN      
        1 agagataaac ggaactgtac tgccggggat catggtatcg ggtagcttac cgtcccaacg
       61 ctcagcagtg gtgagggcaa caagtccggg attatcccgc agagcttcac cgcgcaggcg
      121 gatcgcttct gcttcagccg cactttttaa acgaatagtt tcagcctctg ccgcgcctct
      181 tacgcgaata gtctcagctt ctgctttggc tgccgccagt ttactgtctg cttctgcctg
      241 ggcctgagtt accgctattt gcgcctggat tttctctgtt tccagatttt gcttacgtgt
      301 ggcaatggcg acttctgctt tcattcgatc ttcaatcgat ttttcataag cgtcagaaaa
      361 atcaatattt tcaatctgga caccatcaat caccaccggg ccaacaacgg ctttacgcat
      421 tgcattttgt agatcctgaa ccagttttgt acgatcctgt acggcgctga tggcggtata
      481 ttggccgaaa acgttctcca gttgagttgg caactgacgg acaatcagac gatcttttaa
      541 tgcttcaatg gtgttgtagg tggtataaac cgcgcctgct tctgaaggtt taatgtggaa
      601 gctgactgat actgtcattt gtgccggttg ctggtcgcgg ctgtaggcct gtaatccctg
      661 ataaaccaca gcctgattac gcgtggaaat tttctccacg ctttccataa aagggatctt
      721 aaaacccaga cccggctcgg caacttttac gatttttccg tagcgcagca aaatgccgcg
      781 ttcaccttca ttaacggtgt aatacgagag aaatggcagc acaaccacgg caagcacgcc
      841 aatgacgata gcaatagatt tttgtgggcg aaatgaggtt attgaaacag gtgctttcat
      901 atgaatcttc cttgagtaaa gtggggaata tgtcacatga ggtgaggaga aaatagccga
      961 caggtgagaa ttcagaatct ataaaggggt atagcgaaaa aacattgcat tagaaaagta
     1021 tgctttcgga cattcaataa ataa
//
LOCUS       DAZKGF010000429         1032 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZKGF010000429 DAZKGF010000000
VERSION     DAZKGF010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.432"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1032
                     /locus_tag="ACHOQV_005287"
     CDS             <1..>1032
                     /locus_tag="ACHOQV_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633488.1"
                     /translation="MWLVRGGKAAQPDGHTLGRLWGALPPDIRLSPHLYLATSSAQGP
                     WWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGRTLTYRREAAGDLAGEITGVTDG
                     AGREFRLVLTTQAQRAEEARTSSLSSSDRPRPLSVSAFPDTLPGTEYGPDRGIRLSAV
                     WLMHDPAYPESLPGAPLVRYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVAHRYA
                     GRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRITVTDSLNRREVLHTEGGAGLKRV
                     VKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYY
                     NDGNQLTAVV"
BASE COUNT          186 a          307 c          384 g          155 t
ORIGIN      
        1 atgtggctgg tgcgcggtgg taaggcagcg cagccggacg gccacacgct ggggcggctg
       61 tggggggcgc tgccgccgga tatccggtta agcccgcatc tttacctggc gaccagcagc
      121 gcacagggac cgtggtggat actggggtgg tcagagcggg tgccgggtgc tgaggacgta
      181 ctgccagcgc cgctgccgcc gtaccgggtg ctgacggggc tggcggaccg cttcgggcgg
      241 acgctgacgt accggcgtga ggccgccggc gacctggccg gggaaatcac cggcgtgacg
      301 gacggtgccg ggcgtgagtt ccgtctggtg ctgaccacgc aggcgcagcg tgcggaagag
      361 gcccgcacct cttcgctatc ttcttctgac cgtccccgcc ctctctcagt ctcagcgttc
      421 cccgacacac tgcccggtac cgaatacggc cccgacaggg gtatccgcct ttcggcggtg
      481 tggctgatgc acgacccggc atacccggag agcctgcccg gtgcgccact ggtgcggtac
      541 acgtatacgg aagccggtga actgctggcg gtatatgacc gcagcaatac gcaggtgcgc
      601 gctttcacgt atgacgcgca gcacccgggc cggatggtgg cgcaccgtta cgcgggaagg
      661 ccggagatgc gctaccgcta cgacgatgcg gggcgggtgg tggagcagct gaacccggca
      721 ggcctgagtt accgctacca gtatgagcag gaccgcatca ccgtcaccga cagcctgaac
      781 cggcgtgagg tgctgcatac agaaggcggg gccgggctga agcgggtggt gaaaaaagaa
      841 ctggcggacg gcagcgtcac gcacagcggg tatgacgcgg caggaaggct cacggcacag
      901 acggacgcgg cgggacggcg gacagagtac ggcctgaatg tggtgtccgg cgatatcacg
      961 gatatcacca cgccggacgg gcgggagacg aaattttact ataacgacgg gaaccagtta
     1021 acggcggtgg tg
//
LOCUS       DAZKGF010000430         1032 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZKGF010000430 DAZKGF010000000
VERSION     DAZKGF010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.433"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(456..1028)
                     /gene="hdeD"
                     /locus_tag="ACHOQV_005288"
     CDS             complement(456..1028)
                     /gene="hdeD"
                     /locus_tag="ACHOQV_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFP9633489.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
BASE COUNT          351 a          230 c          203 g          248 t
ORIGIN      
        1 tcgacttagg ctggcaattg gattgccagc tttcttttaa cgcattgaaa ttacctgatt
       61 aaataactca ttcatgtctt caggttttta tatagcgtct ggagacacgg atatttatgc
      121 aatgtaatca acgccagttg caaaaaaatg aacccggatt acttatttaa ccgcccatag
      181 catcaacgct tacggaactc tttgttcact ttcccacagc acttttaaaa cattaaacct
      241 gaccacggaa aatatcagcc atgataatgt ttgaaatggc taatttgcca tggaacgaaa
      301 aaaatcagat taaattcagt aggttgaaat aatcaacagc aggcaattgt ttcaatgata
      361 gtgcgtaatt gaactgaaac actgtatagc taagagagta ttaactggat catagtcact
      421 taatgtgact atgatccagg tggaaatagg gataattatt gctttttaac gaacaaactg
      481 gcgaagctga acagactggc ggcgctaaat atcagttcaa ttcccaccag tgtggaaacc
      541 agcgttaccg acaccatcgg cgtagcacca aggaatatcc aggcaatgac gatatccagc
      601 acaccaataa cgagctgtag ccaactgcct ttcattgaac gctgacgata ccaactcatc
      661 aggcgaataa cccctgccac acagaacaaa ccggcaataa atgccgcaat ggcaaaaatg
      721 cccagctctg gtgcgcggat gaagaaatag ccgatcaata aataggcgac tgcgacgagg
      781 aaaccggata ataccggcca gaaattatga ctgcggttgc tgaatagccc gacaataagc
      841 gcaatgcccg agcagattaa taatgcaccc actactgtgc ttaaaatgtc gccagagacg
      901 aacggaaaac tgatacacag caacccgacg ataaacagca gcacggcaat aaactggatt
      961 gctctgcgat gttttttaag catctccaga tcaaacttca aaattgttgc cttatctata
     1021 tataacatag aa
//
LOCUS       DAZKGF010000431         1030 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZKGF010000431 DAZKGF010000000
VERSION     DAZKGF010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.434"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(59..949)
                     /locus_tag="ACHOQV_005289"
     CDS             complement(59..949)
                     /locus_tag="ACHOQV_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HFP9633490.1"
                     /translation="MVISENTVGTNDIPDSNHDILGPLMPARGFFFIVIDEPGKDGFL
                     VRKIYASPNSPYLTKMNLDPLRLLSETEPERYGLMPKDYSAPTTVAEHVMGNNKSSLI
                     STSSIFPDGSPRFEGKTIFIDVHKAKNAGLKLISTAEIITALEEYKKQYPHLAKRVEK
                     ISGYVDDIDKEVLIEGRKVPAQTIFTPESLKTTQNIVKAGRVVQVFSIFFTAYDLEQA
                     TEKSFETKSIKPIGAEAIRQIGGWGGAVAGAKIGTVIGASLGVTTGPGAVATGIIGGI
                     VFGAAGYFGADWVADYIDEN"
BASE COUNT          290 a          238 c          170 g          332 t
ORIGIN      
        1 aaaaaaatct ttaacttcag gttttttaac gagcaaattc ttcatgtgct ctatccaatt
       61 agttttcatc tatataatcc gctacccaat ctgcaccaaa ataaccagct gcaccaaaaa
      121 caatcccgcc aataatcccg gtagccactg ctcctggccc agtagtaaca cccagggacg
      181 cgcctatcac tgttccaatt ttagcaccag ctactgctcc tccccatcct ccaatttgcc
      241 ttattgcttc tgcacctata ggttttatgc tttttgtttc aaacgatttt tcagttgcct
      301 gttctaaatc atatgcagtg aaaaaaatac taaaaacttg tacgacacgc ccggccttaa
      361 caatgttttg agtcgttttt agagactctg gtgtaaaaat tgtttgtgct ggtacttttc
      421 taccttcgat caagacctct ttatcaatat catcaacata gcctgaaatt ttttcaacac
      481 gcttagccaa atgagggtat tgtttcttat actcctcaag agcagtaata atctctgcgg
      541 tcgagattaa cttcaacccg gcattttttg ctttgtgaac atcaataaat atggttttcc
      601 cctcaaatct cggagagcca tcaggaaata tagagcttgt agaaatcaac gaggatttgt
      661 tgttccccat aacatgttct gctacagtag tgggtgctga ataatctttt ggcatcaacc
      721 catagcgttc aggctctgtt tctgataata atctaagagg atccagattc atctttgtca
      781 ggtaggggct atttggtgaa gcatatattt ttctgacaag aaagccatct tttcccggct
      841 catcgataac tataaaaaag aaacctcttg caggcattaa gggtcctaat atatcgtgat
      901 ttgaatcagg tatatcattt gttcctactg tattctcaga tatcaccatt tttccagcgc
      961 cttaatacca agaagtacgt ttaggttatc agcttcttcc gtgaaaattc tttcacagca
     1021 cccggcagaa
//
LOCUS       DAZKGF010000432         1030 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZKGF010000432 DAZKGF010000000
VERSION     DAZKGF010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.435"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..342
                     /locus_tag="ACHOQV_005290"
     CDS             61..342
                     /locus_tag="ACHOQV_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633491.1"
                     /translation="MTEAELLRLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
BASE COUNT          313 a          231 c          246 g          240 t
ORIGIN      
        1 acgggccata attccaggaa ggacccgacg acaccagact atcagaacga tgggggcaca
       61 atgacagaag ccgaactact acgattaatc cgtcgcgttg ccggaatcag ccagcaggct
      121 gacgaacagg ccacgcagcc ggacagcgtg accgccgaaa attatgcgcg tgtggttgct
      181 gaggtgatgc gccgtgacgg tattgagctt aacggcgtgg atatgcgcga catacggatc
      241 cgcgttctgg aaatgctggc ctacaatcgc cgcgttgaac tgtatcggga gaaagaaaaa
      301 attacgtacc actggaagaa gccggagcgg ttacggcgat aactcactga gattaaagca
      361 aagcgcaaaa ttgcgctggc tacatccctc acatctgagg actgttaacc agcaggccgg
      421 aatatattca caaactacga aattttcgta gtttaattac tcgccagttt gatcgttaac
      481 tcgttgatat ttttcggtaa cctcaatttg aggagaccgg agatcgtgac ggagagtgac
      541 aaccagtgac acaccgtgac aaaaagacgg tttaaccgtt cattaaaaat cgacgtgatt
      601 ttgtgctttc cggatgacgt ggccaacgtc attttttagc gaaaaattct gattaaggtc
      661 attggctgaa tggtcaaaat gaccactcag accatcaagg caaccataac gaacatcgtt
      721 acggttggag cttttacccc attgggggaa aggtattacg ataatcataa cacctaccga
      781 acaggtaaag cccaccagcc tgattaactg ttaaccggaa aaaatccggt agggttcccg
      841 atatggggat ccccatatcg gggaacatcc accagcttca taacggatag ccggaaaatg
      901 ataacggata actacgaaat tttcgcagtt taataaacct ctgggaaaac tcgcctttaa
      961 attgtacact ttttcagcaa atgattttag ttatacgttt ggttatacga tttaaaagtt
     1021 gaatcaagca
//
LOCUS       DAZKGF010000433         1025 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZKGF010000433 DAZKGF010000000
VERSION     DAZKGF010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.436"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            77..>1025
                     /gene="phoE"
                     /locus_tag="ACHOQV_005291"
     CDS             77..>1025
                     /gene="phoE"
                     /locus_tag="ACHOQV_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP9633492.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNK"
BASE COUNT          303 a          213 c          256 g          253 t
ORIGIN      
        1 caggttaaaa actttcctgt tttcaacggg actctcccgc tgaatattcg cgcgttaatt
       61 aaaatcagga atgaaaatga aaaagagcac tctggcatta gtggtgatgg gcattgtggc
      121 atctgtatcc gtacaggccg cagaaatata taacaaagac ggtaataaac tggatgtcta
      181 tggcaaagtt aaagccatgc attatatgag tgataatgac agtaaagatg gcgaccagag
      241 ttatatccgt tttggtttta aaggcgaaac acaaattaac gatcaattga ccggttatgg
      301 tcgctgggaa gcagagtttg ccggtaataa agcagagagt gatactgcac agcaaaaaac
      361 acgtctcgct tttgccgggt tgaaatataa agatttgggt tctttcgatt atggtcgtaa
      421 cctgggcgcg ttgtatgacg tggaagcctg gaccgatatg ttcccggaat ttggtggcga
      481 ctcctcggcg cagaccgaca actttatgac caaacgcgcc agcggtctgg cgacgtatcg
      541 gaacaccgac ttcttcggcg ttatcgatgg cctgaactta accctgcaat atcaagggaa
      601 aaacgaaaac cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt cattgacata
      661 tgactttggt ggcagcgatt tcgccatcag tggagcctat accaactctg atcgcaccaa
      721 cgagcagaac ctgcaaagcc gtggcacagg caagcgtgca gaagcatggg caacaggtct
      781 gaaatacgat gccaataata tttatctggc aactttctat tctgaaacac gcaaaatgac
      841 gccaataact ggcggctttg ccaataagac gcagaacttt gaagcggtcg ctcaatacca
      901 gtttgacttt ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga aagatattga
      961 gggcatcggt gatgaagatc tggtcaatta tgtcgatgtt ggtgctacgt attatttcaa
     1021 caaaa
//
LOCUS       DAZKGF010000434         1018 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZKGF010000434 DAZKGF010000000
VERSION     DAZKGF010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.437"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1018
                     /locus_tag="ACHOQV_005292"
     CDS             <1..>1018
                     /locus_tag="ACHOQV_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633493.1"
                     /translation="FAIKAGDPIGHMGYYQAPKDGGYEARYQVHIECTSMDDNLEKFL
                     TNPEQVGKNNPLWLKYAPGLALYKKDVFTETFTKDTRVTARTGILPLKQVQTEVDKST
                     KQEYWQLRPENAYVPKGQVELQLLSQYDLAKLGFRTEIAEPTSFDYLDGKKQPTGFFR
                     SLINSLYEAANDDTRTSHALVKHNYQRLLDKIDSGSDRYSPMEYWRALHNPDYRDVLQ
                     KTIVKHPSDWYFKKDDAIWQPFLNALKKDAPEWKKYSEDFLDKMAWMQDVTTEKLGPL
                     LWHMHPIMFLGCMKKSSGIWPLGKTSERYESGGRGPGVISSGKGDHGGVSYGMYQLSS
                     KMGTV"
BASE COUNT          313 a          207 c          265 g          233 t
ORIGIN      
        1 tttgccatta aggccggtga cccgattggg cacatgggct actatcaggc accaaaggat
       61 ggggggtatg aggcacgata tcaggtacat atcgaatgca cgagtatgga tgacaatctg
      121 gagaaattcc tgaccaatcc tgagcaggtg ggaaaaaata atccactctg gctgaagtac
      181 gccccggggc ttgcgttgta caagaaagat gtttttacag aaacattcac gaaggacacg
      241 agagtgacgg ccaggacggg aattctgcca ctgaaacagg tgcaaacaga agtcgataag
      301 tcgaccaaac aggaatactg gcaattgcgg ccagaaaacg cgtacgtgcc aaaaggtcag
      361 gtggaacttc aacttttgtc acagtatgat ctggcaaagc tggggttcag aactgaaatc
      421 gcagagccta ccagctttga ttacctggat ggaaaaaaac agcccactgg attcttccgc
      481 agtcttatca actcactcta tgaggctgcg aacgatgata cgcgaaccag tcatgcgctg
      541 gtgaaacata attatcagcg cctgctggat aaaattgata gtggaagcga ccgttactct
      601 ccaatggaat actggagagc acttcataac cccgactatc gtgatgttct tcagaaaaca
      661 atcgttaaac atcccagcga ctggtacttc aaaaaagacg atgcaatctg gcaaccattt
      721 ctgaatgcgc tgaaaaaaga tgctccggaa tggaagaaat acagcgaaga ttttcttgat
      781 aagatggcct ggatgcagga tgtgaccacg gagaaactgg gccctttgtt gtggcatatg
      841 catccgatta tgttcttagg ctgtatgaaa aaatcgtcag gaatatggcc acttgggaaa
      901 acttcagagc gttatgagtc tggaggaaga gggccaggtg taatttcatc tggaaaagga
      961 gatcatggcg gtgtgtcata tggaatgtat caattatctt ctaagatggg aacggtta
//
LOCUS       DAZKGF010000435         1013 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZKGF010000435 DAZKGF010000000
VERSION     DAZKGF010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.438"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..421
                     /locus_tag="ACHOQV_005293"
     CDS             <1..421
                     /locus_tag="ACHOQV_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9633494.1"
                     /translation="QNLTINDPPERQPDGRREQRLTLPEGDLGEWLRSLTPTERKQLT
                     RQLKNPPPDTETVKNGGISAPAGDVRVSVALTAEPHITAQITRELDALGVQLHESAVV
                     SIRHGARVRLADGRTAYWNDSTRRIDITPAPNTEAPQ"
     gene            418..822
                     /locus_tag="ACHOQV_005294"
     CDS             418..822
                     /locus_tag="ACHOQV_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633495.1"
                     /translation="MTRFYCAMQRQRFDELMRQRTPLLTLNNIATSPQPGDELEITVV
                     SPDRTTTAIITDILNGRPDNTNRSWLVAVRPRRPPGEPEPLTPAGKIMQQYHEEQAHK
                     HNAELTRTLVTINRKAAIARYVHSAASRQSRR"
BASE COUNT          279 a          325 c          265 g          144 t
ORIGIN      
        1 ccagaatctg accataaacg acccgccaga aaggcagccg gacggacgca gggagcagcg
       61 acttaccctg ccggaaggtg atttgggtga atggttaagg tcactgaccc ccacagagcg
      121 aaaacaactc accaggcagc tgaaaaaccc gccacctgac acggaaaccg tgaaaaacgg
      181 cggcatcagt gcgcccgctg gcgacgttcg cgtgagtgtc gctctgaccg ctgagccaca
      241 catcaccgca caaatcacac gcgagcttga tgcgctgggc gtgcagctgc atgaaagcgc
      301 cgtcgtttcc atccgtcacg gtgcccgcgt gcgcctggct gacgggcgca ccgcgtactg
      361 gaatgacagc acccgccgca ttgatatcac ccccgcaccg aatacggagg cgccacaatg
      421 acccgctttt attgcgccat gcagcgacag cgttttgacg aactgatgcg ccagcgcacg
      481 cccttactga ccctgaacaa cattgccacg agtccgcaac caggcgatga gctggaaatc
      541 acggtcgtca gtcccgatcg caccaccacg gccatcatca ccgacatact caacggacga
      601 ccggacaaca ctaaccgcag ctggcttgtc gccgttcgtc cgcgccgtcc acccggggag
      661 ccggagccgc tcacgcctgc cggaaaaatc atgcagcagt accacgaaga gcaggcacac
      721 aaacacaacg cagaactcac cagaacactg gtcacaatta accgcaaagc ggcaattgcc
      781 cgctatgtgc attcagccgc cagcagacaa tcacgcaggt aaacaaagac catgaacatg
      841 accagaaaca gccaactaaa ggagcgcaca atgaacagca ccaggaggat cagcgacaga
      901 gagcttacga aaatgattga gtcggcccgg caacttcttt cacgacaaaa cccggtcgtg
      961 gaagaaacat ggtgggagaa tttactggca gcactgaccg aactccggga acg
//
LOCUS       DAZKGF010000436          994 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZKGF010000436 DAZKGF010000000
VERSION     DAZKGF010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.439"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            372..447
                     /locus_tag="ACHOQV_005295"
     tRNA            372..447
                     /locus_tag="ACHOQV_005295"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:405..407,aa:Ile,seq:cat)
BASE COUNT          312 a          177 c          206 g          299 t
ORIGIN      
        1 atacgaaact aatgattagc agaataagtt ctataaaaga atatattgct atatgaagtt
       61 tatttttatg gagtgaaaag taacagatat catcatgatg cgaaaactct tcagctaatg
      121 taataaagtt atattattag atacaaaatc attacaaact atcattgaat cgatgataac
      181 tatagatagt gcttattatt aacatgcttg ttgactgcga gtatcgactt tgcgaatgga
      241 agcggtgtcg ggatttatgt cgcatgtatt gacgacccag gatggatgag aacgcataaa
      301 aatctgttat ttacatcata agatcatcat gtttattgcg tggtttataa gtattgttat
      361 tgcatactta aggcccttta gttcagtggt tagagcaggc gactcataat cgcccggtcg
      421 ctggttcaag tccagcaggg gccaccaaat catcattcat gtatcaagaa gagttaaggg
      481 tacaaggggt actcgatggc ggtctatatt tcatgactaa cgccatttaa aggaagaagt
      541 gcggtgggtg gtttcgaatg agggctggtg ttttggtacg tggcacgcca ctgcaatgta
      601 tttatccatc agtactcatt caaaagtacc tggaaaaaat gcccgctatg ccaagcgggc
      661 aaattgctta catgtggaat taagtaatta acgataaagc cagagtatta tcatttatca
      721 tgcctcttca tttaatcagg ttaattactc aaatcactga actattctct gttcgctcac
      781 tgatgataag tgaagtgtca caaccacgat ttgtatgtgg accaatgccg tagagtaagg
      841 atgaaatgtc tctgccacgc cttaaatatc tactactgac cagaacaccc gccgcaggca
      901 gatgagtctt atgagcgtta caggagtctg gctttgctca ttaattaacg ctatttagat
      961 ataaatttct aacactcgaa aaacgaattc cact
//
LOCUS       DAZKGF010000437          991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZKGF010000437 DAZKGF010000000
VERSION     DAZKGF010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.440"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(103..957)
                     /gene="rclR"
                     /locus_tag="ACHOQV_005296"
     CDS             complement(103..957)
                     /gene="rclR"
                     /locus_tag="ACHOQV_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFP9633496.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT          267 a          262 c          227 g          235 t
ORIGIN      
        1 aaatatataa agcccaaaac caccgttgga aatagaaata gttgaaaggc tcacaactcc
       61 cggaatgcgg attatcttta ccctgatttc tggcggtttt attcagggtg caagctgtct
      121 caccctttcc cgatattctc ccggagtaca accaaactcg cggacaaacg ccttgtgaaa
      181 agatgattca ctggcatagc ctactgactc agcgatcacc acaacaggga gcatttcccg
      241 ggaaaacatc tgggccgcta tttgtagacg caactttgtt aatacagcca gcggcgtggt
      301 tccggaaaca tcacggaaaa gctgggcaaa acttgcccgg gacatgtggg cgatgctggc
      361 cagcgattcg acggtccagg cgtgtcctgg catttccagc atttgctgta ttaccgcacc
      421 aagacgtgga tgcagaagca aactgagaat gtttttctct gtattaacct gtgcaatcca
      481 ctcacgcacc gcgagggtaa agaatgtagc gcagatctgg ctacacagag catccactcc
      541 cggcattgcc gatctggatt cctgttgtaa aaacgggatc gcctccctca gccagttata
      601 ttccacgcta tggttcactg gtgctacaaa cagcgtttcc ggcaaagatg ttaaaaaata
      661 acgcgccgaa tgttgcagcc gaagagtgcc acagacaata caggtcgatt cattatcgac
      721 atgactcagg cgatgagctg aattttgtgg tagcaggacc acatttcccg ggcgtaatgt
      781 aaaaatctcc cccgtcggca tttccagctt cgccgctcct tgcgttaacg catgccaacg
      841 aataaccgac aattccccgg caccatgtgg aagttgccag tcacttccta acacgcaatt
      901 cttatcgatc gttccttgtg gagcgttaag catcaacaaa cggctaaggg catccatatc
      961 agactcctga gtggtttttg attacaaaca c
//
LOCUS       DAZKGF010000438          989 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZKGF010000438 DAZKGF010000000
VERSION     DAZKGF010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.441"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..214)
                     /locus_tag="ACHOQV_005297"
     CDS             complement(<1..214)
                     /locus_tag="ACHOQV_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HFP9633497.1"
                     /translation="MQKYKKRISILTKSEINELYQVPSFNPVERDEFFSLDISLKREI
                     DKIINIESRVYLILITGYFRYKPVIPE"
     gene            371..952
                     /locus_tag="ACHOQV_005298"
     CDS             371..952
                     /locus_tag="ACHOQV_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9633498.1"
                     /translation="MALLGYARVSTSHQKLTHQISELKNAGVREDRIFTDMMTGATDE
                     REGLQRLLARAEKDDVIICTKMDRLGRNTADMIRVVDSCYKKGIAIRFLENGLSTEGT
                     MGKMVIQILAAVAEAERERILERTNDGRIAALAAGVKFGRKKHPRTPTALELISQGES
                     LGSVTEKTGISRSTYFRLKRTIKNDAKIATFYK"
BASE COUNT          329 a          177 c          212 g          271 t
ORIGIN      
        1 attcaggaat cacaggttta tatctgaaat accctgttat taatatcaga tacaccctgc
       61 tttctatatt tattatttta tcaatttctc ttttaaggct gatatccagc gaaaaaaact
      121 catccctttc aacaggatta aaagagggga cttgatataa ttcatttatt tcactttttg
      181 ttaatatact aattcttttc ttgtacttct gcatacattt accaaaatgc taaatatttg
      241 ttaaatatgg ctagtatcaa atattaagat atctgatacc tgtctaaaac agcataaaag
      301 caggtttcaa aaatcgtatt ttgtacatta tgataccatg cagtgtcaaa aacgataagt
      361 gagactaaaa atggcgttat tggggtatgc acgggtatca accagtcatc agaagctaac
      421 acatcaaatc tcggagctaa aaaatgccgg ggtacgggaa gatcgtattt ttacggatat
      481 gatgactggc gcaactgatg aacgggaagg actacagcgg ttgttggccc gtgcagagaa
      541 agacgacgta atcatctgta caaaaatgga ccgactgggg cgtaacactg ctgacatgat
      601 tcgtgtggtt gatagctgtt acaaaaaggg gatcgccatc cggttcctgg agaacggtct
      661 cagtacagaa gggacaatgg gcaaaatggt tatccaaatc cttgctgctg tagctgaagc
      721 ggaacgtgag cgtattctgg agcggacaaa cgacggaaga atagcggcac ttgccgcagg
      781 cgtaaaattt ggcaggaaga agcatccacg aacgcctaca gcgttggagc ttatatcaca
      841 gggagaatct ctgggcagtg tgacagaaaa aacaggtatc tctcgatcga cgtacttcag
      901 gttaaaaaga acaataaaaa atgatgctaa aattgcaacg ttttataaat aaattcaatt
      961 aattacctaa gcaaggtgag acttttaga
//
LOCUS       DAZKGF010000439          980 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZKGF010000439 DAZKGF010000000
VERSION     DAZKGF010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.442"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..573
                     /locus_tag="ACHOQV_005299"
     CDS             <1..573
                     /locus_tag="ACHOQV_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9633499.1"
                     /translation="MLRLRRAGQITGQHVPEIILLNSHDGTSSYQMLPGYFRAICTNG
                     LVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQ
                     AALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRI
                     HTHAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            628..>980
                     /locus_tag="ACHOQV_005300"
     CDS             628..>980
                     /locus_tag="ACHOQV_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9633500.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQSQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIV"
BASE COUNT          226 a          267 c          269 g          218 t
ORIGIN      
        1 atgctgcgtc tgcggcgggc cggacagata accggtcagc atgtgcctga aattattctg
       61 ctcaactccc atgacggtac ctccagctac cagatgttac ccggatattt tcgtgccatt
      121 tgtaccaatg gcctggtctg cggtcagtcg ctgggagaag tccgggtgcc acaccgggga
      181 aacgtggtgg acagggtcat agaaggcgct tacgaagtgg tgggcgtgtt tgaccggatt
      241 gaggaaaagc gtgatgccat gcagtcgctg gtcctgccgc caccggcacg ccaggcgctg
      301 gcacaggcgg cactgactta ccgttatggt gacgaacatc agcccgtcac caccgccgac
      361 attctgacgc cacgacgccg ggaggattac ggtaaggacc tgtggagtgc ttatcagacc
      421 attcaggaga atatgctgaa aggtggaatt tcaggtcgca gcgctaaagg aaaacgtatc
      481 catacccatg ccattcacag cattgacact gacattaagc tcaaccgcgc attgtgggtg
      541 atggcggaaa cgctgctgga gagcctgcgc tgattcagcc attaccctga aaaccacgca
      601 tttatttgtc ctttaaggag tcatttcatg aaaaccattt cacataattc caccacatct
      661 tctgtttccg taaccgctgc atcagggaat gaccagtctc agctggttgc cactcccgtc
      721 cctgatgaac agcgcatcag cttctggccg cagcattttg gcctcattcc acagtgggtg
      781 accctggagc cccgtatctt cggctggatg gaccgtcttt gcgaagacta ctgcggtggt
      841 atctggaatc tgtacacact gaacaacggt ggcgcattta tggcccccga accagatgac
      901 gatgatggcg aaaactgggt actgttcaat gccatgaacg gtaaccgcgc tgaaatgagc
      961 ccggaagccg ccggcattgt
//
LOCUS       DAZKGF010000440          974 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZKGF010000440 DAZKGF010000000
VERSION     DAZKGF010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.443"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..667
                     /locus_tag="ACHOQV_005301"
     CDS             <1..667
                     /locus_tag="ACHOQV_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP9633501.1"
                     /translation="GTREVRRFRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILP
                     ELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPS
                     PAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRL
                     QDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRNTVVSVARAVWNW
                     IFF"
BASE COUNT          205 a          270 c          267 g          232 t
ORIGIN      
        1 cggcacccgg gaagtacggc gcttccgtct gagtggccac ggtcacagca tggttatcac
       61 tgacctgccc ggggtgggcg agagccggga cagggatgca gagtatgagg ccctgtaccg
      121 tgacattctg cctgaacttg acctggtgct gtggctgatt aaagccgatg accgggccct
      181 gtctgtggat gagtatttct ggcgacacat cctgcaccgg ggacatcagc aggtgctgtt
      241 tgtggtgacg caggccgaca aaacggagcc ctgccatgaa tgggatatgg ccggtattca
      301 gccttctcct gcacaggcac agaatattcg cgaaaaaacg gaggcggtat tccgtctgtt
      361 ccggcccgta catccggttg tggccgtatc ggcccgcacc ggctgggaac tggatacgct
      421 ggtcagtgcg ctcatgacag cgcttcccga ccatgccgcc agtcccctga tgacccgact
      481 gcaggacgag ctgcgcacgg aatctgtccg gtctcaggcc cgtgaacagt ttaccggtgc
      541 ggtggaccgg atatttgaca cagcggagag cgtctgtgtt gcctctgttg tccgtacggc
      601 cctgcgcgct gttcgtaaca cggtggtctc tgttgcccgt gcggtatgga actggatttt
      661 cttctgaacc tgtcgtgact gatgccctcc ctgactctga gtctgctcac aaaagcgctg
      721 ttttcgttac tgtctctctt gtccgtgcaa tagccaaaca atagaataaa acgatcgata
      781 cctattttat cgatcgttta tatcgatcga taagctaata ataacctttg tcagtaacat
      841 gcacagatac gtacagaaag acattcaggg aacaacagag ccacacgtca gaaacttccg
      901 tcagccggac ctctggcact gtaacccttt acctgccggt atccacatct gtggataccg
      961 gcttttttat tcac
//
LOCUS       DAZKGF010000441          973 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZKGF010000441 DAZKGF010000000
VERSION     DAZKGF010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.444"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..808
                     /gene="gadX"
                     /locus_tag="ACHOQV_005302"
     CDS             <1..808
                     /gene="gadX"
                     /locus_tag="ACHOQV_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFP9633502.1"
                     /translation="NCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQVDKCVEN
                     FVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQNKNLRY
                     AEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIAS
                     ELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGYHSVSYF
                     IYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
BASE COUNT          280 a          179 c          219 g          295 t
ORIGIN      
        1 taattgtcta attgcgtacg caagacataa atatattctc accatggtta atggtgaata
       61 tcgctatttt aatggcggtg atctggtttt tgcggatgca agccaaattc aagtggataa
      121 gtgtgttgag aatttcgtat tagtttcaag agacacgctt tcattatttc tcccgatgct
      181 caaggaggag gcattaaaac ttcatgtaca taaaaaagtt ccttcattac tcgttcatca
      241 ctgtaccaga gatattcctg tttttcagga agttgcgcaa ctatcgcaga ataagaatct
      301 tcgctatgca gaaatgctac gtaaaagggc attaatcttt gcgttgttat ctgtttttct
      361 tgaggatacg cagtttatac cgttacttct gaacgtgttg caaccgaata tgcgaacacg
      421 agtttgtacg gtcatcaata ataatatcgc ccatgagtgg acattagccc gaattgctag
      481 cgaattgttg atgagtccaa gcctgttaaa gaaaaaattg cgcgaagaag gtacgtcata
      541 ttcacagttg cttactgagt gtaggatgca gcgtgcttta caacttattg ttatatatgg
      601 ggtttcaatt aagcgagttg cagtatcctg tggatatcac agcgtgtcgt atttcattta
      661 cgtttttcga aattattatg ggatgacgcc cacagagtat caggagcgat cggcgcagga
      721 attaccgaac tgtggctcgg cggcaagtat ggctgcgcaa gggatttttt acggcactga
      781 ccgttctgcg gaaggaataa gattatagag ttttactcag acataaaaaa aacccggcat
      841 aggggaccgg gaagaggata gtctgccgtc tccagactaa taaaccgtta taacactccc
      901 tgttggcacg ggaaactttg tgctctcagt aagttaaata taacttttac tggaaataag
      961 atcagccatt ttt
//
LOCUS       DAZKGF010000442          965 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZKGF010000442 DAZKGF010000000
VERSION     DAZKGF010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.445"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..894
                     /locus_tag="ACHOQV_005303"
     CDS             <1..894
                     /locus_tag="ACHOQV_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFP9633503.1"
                     /translation="TLKIQVSSRGIEQVNFLNQRPLLDAKQQPFVTNTVDAEGLALTH
                     NGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNISSDKESSNGIRSGNAWEGLT
                     VTPDGKSLFIAVESSLKQDGPIASPINSGTSRLLQFSIDADGRPSKQLHEYLYITDPV
                     PQVSKFGINDNGVSEVMALNDHQLLVIERSGRNVSAGFNDWDYSVRVYMVDLTAASDI
                     KNIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCIEGVTFGPLIDGHTSLIFVSDNN
                     FQPHQQTKFYLFIDKENKLKI"
BASE COUNT          292 a          199 c          200 g          274 t
ORIGIN      
        1 acactaaaaa tacaggtatc atccaggggc attgagcaag tgaattttct caaccagcgt
       61 ccactgcttg atgcaaaaca gcaacctttc gtgaccaata cggtggatgc cgaaggtctg
      121 gccctgacac acaatggtaa atcattactc tggagttcgg agttaggtgc tccactgcgt
      181 ctgagcactc tcgatggcgt aatggagaaa gattttacgt ctttatttcc tgctcgcttt
      241 aatattagtt ctgacaaaga gagcagcaat ggtattcgca gcggcaacgc ctgggagggc
      301 ctgacagtta caccagatgg aaaatcactt tttatcgccg ttgaaagcag ccttaaacag
      361 gatgggccta ttgcaagtcc aataaattca ggcacatcac gcttactgca attttctata
      421 gatgcagacg gtcgaccttc aaagcaattg catgaatatc tctatattac tgatcctgtt
      481 ccgcaagtga gtaagttcgg tattaatgat aatggcgtat ccgaggtcat ggcgctgaat
      541 gatcaccaat tgttggtcat cgaacgttcc ggcagaaacg tcagtgctgg gttcaatgac
      601 tgggattact ctgtcagagt ttatatggtc gatttaactg ctgccagcga tatcaagaat
      661 attgacagtc tgcaagattg gtccaataaa tccacgttac aacccgtcag taaaaaatta
      721 ttaattgatt ttgctgatta cacttcctct gcggattgta ttgaaggggt aaccttcggt
      781 cctctgattg atgggcatac atcgttaata tttgtatctg ataataattt ccagccccat
      841 caacagacga aattttatct attcattgat aaggaaaaca aacttaaaat ttaaagtcaa
      901 actatagata caaaaacatt ctaatacatg gaatcatcag atggtgtatt cgatcaccaa
      961 aaata
//
LOCUS       DAZKGF010000443          960 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZKGF010000443 DAZKGF010000000
VERSION     DAZKGF010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.446"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(47..373)
                     /gene="ybfB"
                     /locus_tag="ACHOQV_005304"
     CDS             complement(47..373)
                     /gene="ybfB"
                     /locus_tag="ACHOQV_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFP9633504.1"
                     /translation="MKCIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(373..>960)
                     /locus_tag="ACHOQV_005305"
     CDS             complement(373..>960)
                     /locus_tag="ACHOQV_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9633505.1"
                     /translation="YYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPITNIDPLG
                     LETLKCIKPLHSMGGTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDG
                     LWGPGKASNDTKEAAGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLF
                     KNCQDWSNDSLETCKMKCSGNNIGRFIRFVFTGVM"
BASE COUNT          311 a          217 c          146 g          286 t
ORIGIN      
        1 tatcaatgcc caggttacat ataataacaa gagcaattaa cttaatttat ttgtttataa
       61 tgcgcttact taatacaaac cattgaatat accctcctga cagaaaataa agccatatta
      121 ttatcgccat aatatactta ctatcaatcc ttacgccaat aatgtctgaa acagtcccta
      181 taaatattag agcaataaag aatactatgc ctgttggaaa acatattacc atcattccat
      241 aagacatgag actaattaac tcgctcacat cacgagtgga gtctaatagt gaaagtctat
      301 gcatggaaaa gaatagtatt aaaagcgata aagccagcca tatagctctg aataaaaaaa
      361 taatacattt cattacatca ctccggtaaa tacaaatcta ataaaacgtc caatgttatt
      421 tccggagcac ttcatcttac atgtttctaa agaatcatta gaccagtctt ggcagttttt
      481 aaatagccct aaatttatag gtgtgaatat atcaggcaat acagaataac gcggccttac
      541 ctctttaaac ttccctttta aacagttctc cacacaacta ttatcggttt caacgaggtc
      601 acaacggcca gcagcttctt ttgtatcatt acttgcttta cctggccccc atagaccatc
      661 tccttttgat tctccccgtt ggtcttggcc accacaagta tagtcccctt taccatctgg
      721 gacacaaaga tattgatgat agaacggatt cccccatata tctggaccgc ttctttcacc
      781 agttccgccc attgaatgca gtggcttaat gcattttagt gtttctaatc ctaaaggatc
      841 tatattagtg atcggattca acggatactg ataaaaattc catcccccct taagcccaat
      901 cggatcctga gtgatatacc gcccctgcag cgggtcataa tagcggtggc ggttgtaata
//
LOCUS       DAZKGF010000444          948 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZKGF010000444 DAZKGF010000000
VERSION     DAZKGF010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.447"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            68..916
                     /locus_tag="ACHOQV_005306"
     CDS             68..916
                     /locus_tag="ACHOQV_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9633506.1"
                     /translation="MNQTLPTADLNTDGTTDVIPSVAIDRIIAQRNEGISLFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEHGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
BASE COUNT          242 a          216 c          262 g          228 t
ORIGIN      
        1 aggagcttcg ctcctccggg aaatgacctg gctgacccgg cagtcgtgac gatgaggaga
       61 caatgtgatg aatcagactt tacccactgc tgacctgaat actgacggca cgacagatgt
      121 tattccgtct gtggctatcg accgcatcat cgcgcagcgt aacgaaggta tttcactgtt
      181 catgcaggcg atggaatgcc tggcgacagc acgcaggatt ctgcttgatg cgtcaggtga
      241 catttttctc tacgggtttg aagactgcgt gactgactcc gttcgatgca tggataaacc
      301 ggaagaagca aaaaagaata tcacccgtct tgccgaccgg aaaatctggg atcgcctgat
      361 gacggatact ggcatgtaca cattcatgag ttcatgccag cgtgatgagt ggaacagcca
      421 gctgatgagc gacacctgtc ctgaaatcac cctggacaat gtactggcaa cttttcgcca
      481 tctgaatgcc agtaagatgc agacatttga acatggactg attgatgtct accggaaatt
      541 gtcatgggat tacagaacca ataatccctg ccgtctggga aagaaaatca ttattgaaaa
      601 cctgctgtac cgctggagta acgggcgtgt gacgctggac tgcagcggac gggaggcact
      661 ggatgacctg gtacgtccgt tttatctgct ggaggggcgc aacgttcctg acttcaggaa
      721 cagcatcggg gcgcagtatg gtgaatttct cgggaacggc gacaatgtcg gtgagttgtt
      781 tgaaggggaa tattttacgg tgcgtggcta ccagaaaggg accgtacaca ttgtctttaa
      841 gcgtcctgac cttgttgaaa aactgaatga tattattgca cggcattatc ctggtgcatt
      901 gccgccacga gtctgattaa acagaaaagc cctggtattt atgccggg
//
LOCUS       DAZKGF010000445          940 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZKGF010000445 DAZKGF010000000
VERSION     DAZKGF010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.448"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..747
                     /gene="ydeO"
                     /locus_tag="ACHOQV_005307"
     CDS             <1..747
                     /gene="ydeO"
                     /locus_tag="ACHOQV_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFP9633507.1"
                     /translation="SVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFK
                     EIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFS
                     ELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISES
                     LLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHF
                     GNSPKRVSKEYRCQRHTGMNTDNTMSALAI"
BASE COUNT          290 a          180 c          181 g          289 t
ORIGIN      
        1 tctgttatat ttattcatca tgccttcaac gctaacattt tagataaaga ttacgccttc
       61 tctgacggcg agatcctgat ggtagataac gctgttcgta cgcattttga accttatgag
      121 cggcatttca aagaaatcgg atttaatgaa aataccatta aaaaatatct acaatgcact
      181 aacatccaga cagtgacggt gcctgttcct gcgaagtttt tacgtgcttc aaatgtaccg
      241 actggattgc ttaatgaaat gattgcttat ctcaactcgg aagaacgcaa tcatcataat
      301 ttttcagaac ttttgctttt ttcttgcctg tctatttttg ccacatgcaa aggtttcatt
      361 acactattaa ctaacggtgt gctatccgtt tccgggaaag tgagaaatat tgtcaacatg
      421 aagctggcgc acccatggaa gctgaaagat atttgtgact gcctgtacat tagtgaaagc
      481 ctgttgaaga aaaaactaaa gcaagagcaa acgacattct cacagattct tttagatgcc
      541 agaatgcagc acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa aattgccgaa
      601 caatgtggtt atgcgagtac atcttatttt atttatgcgt tccgcaaaca tttcggcaac
      661 agtccgaaga gagtttctaa ggagtaccgt tgtcaaaggc acacgggtat gaacacggat
      721 aacacgatga gtgctttagc tatttgatta tttgctaacg agtagtcaac cacgcacgtt
      781 acgtagaaat taaaatgggg cagccattcc ctgccccgcg ttgtttttag gcgatatatt
      841 attgaaataa ataagtgaca ttcatcacat atttatgcat ttgcataacc tgttgcatga
      901 ttatttatga tctcaattcg gaattttgtc agtaaaatac
//
LOCUS       DAZKGF010000446          938 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZKGF010000446 DAZKGF010000000
VERSION     DAZKGF010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.449"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(57..260)
                     /locus_tag="ACHOQV_005308"
     CDS             complement(57..260)
                     /locus_tag="ACHOQV_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633508.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(351..818)
                     /locus_tag="ACHOQV_005309"
     CDS             complement(351..818)
                     /locus_tag="ACHOQV_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFP9633509.1"
                     /translation="MTANTSSANAKTEYFNLTLKGLGYLSNIRQVNHQNGTFLSCVVN
                     ALSGPTDSPSYVRFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTLNR
                     GEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
BASE COUNT          242 a          236 c          210 g          250 t
ORIGIN      
        1 tgaccaatca gtatgacttt attcactcca cgcgatgcca taacaactcc ttatcctcac
       61 acacattcac gctgagtcca gatgccgttt tccagagccc ggactgcctg ctcataattt
      121 ctgaaatact cttcggattc ccgggacaca ggcccttcgt cgcactgagt accaatataa
      181 taaccacgag cactgctgag gatctgcaga ggtaaggtat agcccgcttt ggcggccaga
      241 attccgcttg tcggtttcat aacatgtttc cttaaagtta aacgataaga aaacagacct
      301 ccctgatggt tggtctgttt tcatcaggga ggttgagttg aaaaatacga tcagaaagaa
      361 ttttctgcgt attcccgcgg ggtattgcca ttctgtggcg gtatggattc cgatttttca
      421 gtcttataaa ccatgtcctg gcctattttg atccactcca ccttgatcag gcgggctttc
      481 agactgacgc gttgctcacc ggcatgttca ccccggttaa gggtaaaaat gtcagtagag
      541 gggttgctca tggtaaaacc aatcagcacc ttgcgatctt catccaccgc tttctggcag
      601 cgggcaatca gggaagttgc ctctttaccg gcaaccgtca catcaaaacg cacataggac
      661 gggctgtccg ttggaccact cagtgcattc acaacacaac tgagaaatgt cccgttctga
      721 tgattaacct gacgaatgtt gctgagatac cccaggcctt tcagtgtcag attgaagtac
      781 tcagttttag cgtttgcaga agaagtattt gcagtcataa tgatatctcc attctcagta
      841 tcggaaagac agagaacatc atcatccctg acgggttgtg atattcccgt cagggagccg
      901 gaacagaact attccggctg actcagtaac agttaaag
//
LOCUS       DAZKGF010000447          931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZKGF010000447 DAZKGF010000000
VERSION     DAZKGF010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.450"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          376 a          194 c          134 g          227 t
ORIGIN      
        1 aggaaattat gtaaatgctg agggtacaga aacgtcgtac tacggcatta ataaaagaga
       61 taaccagcag cacagacaac ctgataaaac tgttttgtat tacctcatta cagcaatgag
      121 gtaatatttc agaatacagt gagactctct taaactgagg accagtacaa attacaatgg
      181 taaaaacacc acctcataga attgagttta agaaaacacc attcactcaa cctgacattc
      241 acaaaagtca gtaccaggta gcctcaggta acatcgctat taatattatc ctgccattaa
      301 gtaaaaacag aaactctgtg aggaagcctc accaatattt gaccagtagc ataaagaata
      361 acgatatgag agatacgccc acaccagaga tgattagccc atacaaaaag ataatattta
      421 ttaataccac cgaacctcaa tagagtaata acacactaat agcgttttag tacacactta
      481 acactaacta tgcaccagac acatactcag gattaacatt ctcaacattt atggtactct
      541 caaagagtat aattcaccac acaggtaaac tctcacacca gtagtatgga gataatgctg
      601 tacacatact tacagagtaa agtgtacagc tagcatacag tggcgagcag acattacaac
      661 ataaagactc aagatagcat aataaagcat aataagaaca caacaaaact cccactaata
      721 atactaatta accttaattc ttttttgaac aggaaatcaa catagctcca gacctcttca
      781 ggtcatacac ttttgtattg gatgttcaat gtgtacttct gcacagacaa aaaaataaaa
      841 cccacaaaat aaaatatcaa tcccaataaa aaattttaaa atacagcgtg aaacaccacg
      901 ttatggatat gttttcatat caccagaagc a
//
LOCUS       DAZKGF010000448          926 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZKGF010000448 DAZKGF010000000
VERSION     DAZKGF010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.451"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..439)
                     /locus_tag="ACHOQV_005310"
     CDS             complement(<1..439)
                     /locus_tag="ACHOQV_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633510.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTIEREHSRNILALNELNF
                     GRQPGDFFARTVFVLLHNLCPDCFRIHSTVGGASWSLPLKWIKR"
     gene            complement(521..898)
                     /locus_tag="ACHOQV_005311"
     CDS             complement(521..898)
                     /locus_tag="ACHOQV_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633511.1"
                     /translation="MYSPLIAHYSALQTQSALLAHISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
BASE COUNT          244 a          277 c          223 g          182 t
ORIGIN      
        1 accgttttat ccatttcagc ggcagcgacc agctggcacc accgacagtg gaatgaatac
       61 gaaaacagtc cggacacaga ttgtgcaaca gcacaaagac tgtacgggca aagaaatctc
      121 ccggctgacg tccgaaattc agctcattaa gagccaggat attccggctg tgctcccgct
      181 caatagtgac attcaccgac cacagcccgt cggcagaccg gcagtaaaac gccagcgtgt
      241 ccgttaccgg aaacaactct gacgcccggg tcagggagcc gaaatacgca ttctgcggcc
      301 gtaacgcctc cggcaactgc cagaaggttt cagtgacagc cagaacaaaa gcatgccact
      361 gcttttccgg tattgtggaa acctgctcaa gggtatatac caccggctca cgggccggac
      421 gtgaagataa ctcagacatg gaacattcct catggttaaa acgaaacaac ggggaatgtc
      481 tcccggacgg agaactcccc cgtcagggtc aggtcatgaa ttacatcatg tcgtgccggg
      541 caaaatattc atccgctccg gccaccatct ttccggtact gatactgtca gcccagattt
      601 catgattaat ttcacgcccc agagccatca gactgtcgag tgcctgacgg gcctgaattc
      661 ccggtgcgga aatctcctcc tgctccaggt catacaacag ccagcccagc gcctcgccgg
      721 tatcccacag atacacgtat accagtgaag gctccgtctc atcatccggc tccggggtaa
      781 tacccagccg ttgccaggca cgtaaccgca tcagctcgcc ttccatctga ctgatatgag
      841 ccagcagagc agactgcgtc tgaagcgctg aataatgagc aattaaaggt gaatacatga
      901 tgacctccgt ctcttaaagg aagtcc
//
LOCUS       DAZKGF010000449          925 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZKGF010000449 DAZKGF010000000
VERSION     DAZKGF010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.452"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            47..847
                     /locus_tag="ACHOQV_005312"
     CDS             47..847
                     /locus_tag="ACHOQV_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633512.1"
                     /translation="MNSKKVCCICVLFSLLAGCASQSSIDEKKKKAQVTQSNINKNIP
                     PQLTDKDLFGNETTLAVSEEDIQAALEGDEFRVPLNSPVILVQSGSRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YHDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKMSNKELSNDHVTEVQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          291 a          170 c          215 g          249 t
ORIGIN      
        1 gtgctaaata ttctaatcat tatgacaggc gagggagtgt ccgattatga attcaaaaaa
       61 ggtttgttgc atatgtgtat tattctcgct gcttgcagga tgtgcctctc aatcttctat
      121 tgatgaaaaa aagaaaaaag ctcaagttac acaaagtaat attaataaaa acattccccc
      181 gcaactgaca gacaaagatt tattcggtaa tgaaaccact ctggctgtat ccgaggagga
      241 tattcaggct gcgcttgaag gagatgagtt tcgtgtgcct ctcaattctc ccgtaatact
      301 tgtccagtct ggcagtcgcg caccggaaac cattatgcag gaggagatga gaaaatatta
      361 tactgtttcc acattctctg gtatcccgga caggcaaaag cctctgactt gtaacaaaaa
      421 taaagataaa aatgagaacg aggatgttgc cagtgctgaa aatatgaact ggatgcaggc
      481 attgcgtttt gtggctgcga aagggcatca gaaagcgatt attgtttacc atgatacgtt
      541 gcagacagga aaatatgact ctgcgctgaa atcaacagta tggtccgact ataaaaatga
      601 aaaactcact gacgctatct ccctgcgcta cctggtacgt ttcacgctgg tggatgtggc
      661 aacaggtgag tgggctacct ggtcgccggt gaattatgaa tcgagagtaa tttttccgcc
      721 ggctggaata acaaaaatga gcaataaaga actatcaaat gaccatgtta cggaagtgca
      781 actgtttaat ttgaaacaaa aaacatatgt atcgatggtg aaagacttgg tgagtcgatt
      841 ccagtaatcg ttattgttat gctgccgcct gtagtttagg gcgggcagca aacaggagct
      901 gagtatttct ataatgtaaa tatgt
//
LOCUS       DAZKGF010000450          920 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZKGF010000450 DAZKGF010000000
VERSION     DAZKGF010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.453"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(99..521)
                     /locus_tag="ACHOQV_005313"
     CDS             complement(99..521)
                     /locus_tag="ACHOQV_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633513.1"
                     /translation="MIPSQISFNTLPVNATYASEPTVDTQKFSDILYSAGCSLKDLAY
                     LSRCLASIHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFSILSSLIDSTISKFQHQNPVNGSVNPDLLYEDYTD"
     gene            complement(602..>920)
                     /locus_tag="ACHOQV_005314"
     CDS             complement(602..>920)
                     /locus_tag="ACHOQV_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633514.1"
                     /translation="DFNILTSDEYMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPS
                     GNQIYFHIFPDESASLVLYFSNNDIECFVFERDIPSQYRFFKMFTNLALVIDQLTDAN
                     QIL"
BASE COUNT          290 a          140 c          203 g          287 t
ORIGIN      
        1 agaaaagtac atgaaggtgc taccagtacg agaaacgtta atcaaaagtt ttattacgtc
       61 tggcaataac gcaggccatt tttcgcgaca ggagggattt aatcagtata atcctcatac
      121 aataggtcag gatttacaga accgttaacg gggttttggt gttgaaattt agaaatggtg
      181 gagtcaatca atgaagacag tatcgaaaaa atgactgtgt cagaattaat ttcaattaat
      241 ccttctcgtt gttgaccaaa aaagatattt atcttgattt catttgtact acggcaatca
      301 atatgtagta atttttgatc gctgagattt tctgtttcat aaatgttttt ggcgatagtc
      361 ggatgtattg atgccagaca tctgctcaga taagccagat cctttaggga acaccccgct
      421 gagtagagga tgtcagagaa tttttgtgtg tcaactgttg gctctgaagc gtatgttgca
      481 ttgactggaa gggtgttgaa ggagatttga gatgggatca ttatttaaat ctttccgtga
      541 gtaataaaaa ttaatccccg ggcattgttt agtaatattt gccacagggg atatataaaa
      601 attaaagtat ttgattggca tcggttaact gatcaataac cagtgccaga ttggtaaaca
      661 ttttgaaaaa gcgatactgg ctcggaatat ctctttcaaa gacgaaacat tcaatgtcat
      721 tgtttgaaaa ataaaggact agtgatgctg attcgtcagg aaaaatatgg aaatatatct
      781 ggttaccaga aggaggatag tccagaagaa gggtatcttt gcccgtcaca tacctgttgt
      841 ggtcgtggat gctgcgctca cacttcatca gaggtgtcat gatactcatg tattcatctg
      901 atgttaatat attaaaatca
//
LOCUS       DAZKGF010000451          894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZKGF010000451 DAZKGF010000000
VERSION     DAZKGF010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.454"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..402
                     /locus_tag="ACHOQV_005315"
                     /pseudo
     CDS             1..402
                     /locus_tag="ACHOQV_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            394..729
                     /locus_tag="ACHOQV_005316"
     CDS             394..729
                     /locus_tag="ACHOQV_005316"
                     /inference="COORDINATES: protein motif:HMM:NF026979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain fold toxin domain-containing protein"
                     /protein_id="HFP9633515.1"
                     /translation="MAKTRDLATTISDKFKQLFQCDKFAAALKSKMQQAGVNGEHLIL
                     ETKNKQGAFGNIWSDRIGKNIATNGHHEAIKVGDTVFDNMNPSGIKYEDWLNDIYSPG
                     EYTITPSRF"
BASE COUNT          279 a          175 c          223 g          217 t
ORIGIN      
        1 ccgtcggtgg ccccgcagtg ggtgacgcgg acgaatcatg cggtgagcga cctgacgggc
       61 cgtccgctga tgctctttaa cagtgaaggt aaaaccgtgt ggcgaccgtg acaaaccagc
      121 ctgtgggggc tggcactcag tctgcccgca gacaccggct acccggaccc gcgcggggag
      181 ctggacccgg aagccgaccc cggcctgctg tatgcaggac agtggcagga tacggaatcc
      241 gggctgtgct ataaccggtt ccggtattac gagccggaaa cagggatgta cctggtgagt
      301 gacccgatag ggctgagagg cgggataaac aattatgcat atgttcataa tccccttggt
      361 tatattgatc cattaggatt aaccatttgc gatatcgcaa aaacaaggga tcttgcaaca
      421 acaataagtg ataagtttaa acagcttttt caatgcgata aatttgccgc agcgttaaaa
      481 agtaaaatgc aacaagctgg ggtaaatggt gagcatctca tactcgaaac taaaaataaa
      541 caaggtgcct ttggcaatat atggagtgat agaataggta aaaatatagc aactaatggt
      601 catcatgaag caataaaagt aggtgataca gtttttgata atatgaatcc ttctggtata
      661 aagtatgaag attggctgaa tgatatatac tctcctggcg aatacaccat tactccatca
      721 aggttttaat tatgaatgat attgttaaac tgattgatat gatagaaaaa agaccagctt
      781 tatatttaag caggcatacc atatcggcgt taaaagcttt cctggatgga tggcaaatga
      841 gagagccaaa agaaatcgtc aatattgaga tattaataag gcttcaatct tata
//
LOCUS       DAZKGF010000452          890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZKGF010000452 DAZKGF010000000
VERSION     DAZKGF010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.455"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..419)
                     /locus_tag="ACHOQV_005317"
     CDS             complement(<1..419)
                     /locus_tag="ACHOQV_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFP9633516.1"
                     /translation="MSILTTGAFLALAAQCAPNVHPDTSLDVVRVESGLHQYAIAEII
                     PKNERVAEGKSFISHYPESKDEAVKIIRRIEERKRNYSVGLMQINSSNFKKLRVTATE
                     LLEPCSNLKAYQYIITDCYLRGGSLTNALSCYYSGDF"
     gene            complement(419..664)
                     /locus_tag="ACHOQV_005318"
     CDS             complement(419..664)
                     /locus_tag="ACHOQV_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633517.1"
                     /translation="MSTSNENLSNKKSPIYQIKQIYIKEDTKAQLMEFIKGINDECQL
                     LVKPTYIVHCLLNCALQSEGIRKEVINYVANAREKMR"
BASE COUNT          297 a          192 c          115 g          286 t
ORIGIN      
        1 ataaaatccc cagaataata acaacttaac gcgttagtaa ggctacctcc tcgcaaataa
       61 caatcggtaa taatgtattg atacgctttc agattagagc acggctccaa caattcagta
      121 gctgtcactc taagtttttt gaaatttgaa ctgttaatct gcattaatcc aacagaataa
      181 tttcgtttcc gttcctcaat cctcctgatt attttcactg cttcatcttt actctccggg
      241 taatgactta taaagctttt accctctgca accctttcat ttttaggtat aatttccgct
      301 atagcatatt gatgtaaccc cgactcaaca cgaaccacat caagagatgt atctggatga
      361 acgtttgggg cacactgtgc agccagagcg agaaatgccc cggtagtcag tatactcatc
      421 agcgcatttt ttctcgtgca ttagcaacat aattaataac ttctttcctg attccttcgc
      481 tctgtaatgc acagtttaaa agacaatgaa caatatatgt tggctttaca agtaattgac
      541 actcatcatt tattccttta ataaattcca ttaactgcgc ttttgtatct tctttaatat
      601 atatctgttt tatctgatat atcggcgatt ttttatttga taagttttcg ttagatgtac
      661 tcatatactc tcctgaaaaa tcacttggtg catattaaaa cataacattt caaataacct
      721 atgatttatt gattcatgac tcatcatcag atataaacac ccccataaat aacataaaga
      781 caccaaaaaa agagtccata atccaaataa atgtatagat attatctgta aaatcatcag
      841 ccataaaacc caaaaacgcc acaccaatta atagaaaacc aagccaaaca
//
LOCUS       DAZKGF010000453          887 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZKGF010000453 DAZKGF010000000
VERSION     DAZKGF010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.456"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>887
                     /locus_tag="ACHOQV_005319"
     rRNA            <1..>887
                     /locus_tag="ACHOQV_005319"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          223 a          200 c          294 g          170 t
ORIGIN      
        1 cgagtggcgg acgggtgagt aatgtctggg aaactgcctg atggaggggg ataactactg
       61 gaaacggtag ctaataccgc ataacgtcgc aagaccaaag agggggacct tcgggcctct
      121 tgccatcgga tgtgcccaga tgggattagc tagtaggtgg ggtaacggct cacctaggcg
      181 acgatcccta gctggtctga gaggatgacc agccacactg gaactgagac acggtccaga
      241 ctcctacggg aggcagcagt ggggaatatt gcacaatggg cgcaagcctg atgcagccat
      301 gccgcgtgta tgaagaaggc cttcgggttg taaagtactt tcagcgggga ggaagggagt
      361 aaagttaata cctttgctca ttgacgttac ccgcagaaga agcaccggct aactccgtgc
      421 cagcagccgc ggtaatacgg agggtgcaag cgttaatcgg aattactggg cgtaaagcgc
      481 acgcaggcgg tttgttaagt cagatgtgaa atccccgggc tcaacctggg aactgcatct
      541 gatactggca agcttgagtc tcgtagaggg gggtagaatt ccaggtgtag cggtgaaatg
      601 cgtagagatc tggaggaata ccggtggcga aggcggcccc ctggacgaag actgacgctc
      661 aggtgcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg
      721 atgtcgactt ggaggttgtg cccttgaggc gtggcttccg gagctaacgc gttaagtcga
      781 ccgcctgggg agtacggccg caaggttaaa actcaaatga attgacgggg gcccgcacaa
      841 gcggtggagc atgtggttta attcgatgca acgcgaagaa ccttacc
//
LOCUS       DAZKGF010000454          880 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZKGF010000454 DAZKGF010000000
VERSION     DAZKGF010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.457"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..227)
                     /locus_tag="ACHOQV_005320"
     CDS             complement(<1..227)
                     /locus_tag="ACHOQV_005320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633518.1"
                     /translation="MTTCSELKERARFDGMHDASLEVAWQLLMTKSVSTITTEIISGA
                     DMSNFSVIIRERYKHAIAKSLLNHLSPDVVS"
     gene            complement(311..>880)
                     /locus_tag="ACHOQV_005321"
     CDS             complement(311..>880)
                     /locus_tag="ACHOQV_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFP9633519.1"
                     /translation="IQTIRKIKSGFSKYLLNNSFNKFSLGYNVISGSIHPTKFTNADG
                     NHLHEVLSGDLGVLQDNTKAPLDSYLFEEVFYDSELERLNITDGEIHSVAVFTKIPKN
                     SIKIPVAGGYTYSPDFAYVVKTTKGDYLNFIIETKNVEGKESLRKEELDKIKHAQKLF
                     EQVSKSVKVTFITQFSGDRIHDLIKNVIQ"
BASE COUNT          259 a          157 c          155 g          309 t
ORIGIN      
        1 tcagaaacaa catcgggaga taaatgattg agaagagatt ttgctattgc atgtttatat
       61 cgttccctga taatcactga aaaattggac atgtctgcac cggatattat ctcagtagtg
      121 atagtggata cacttttcgt cataagtaat tgccatgcca cttcaagaga agcatcatgc
      181 ataccatcaa aacgggctct ttccttcagt tcagaacatg ttgtcattga actcacctgc
      241 gaattttgtg atgtgtgtat aattataaag tgggggtgaa accacctcca ctttgttatc
      301 atgcatgtgt ttactgaatc acatttttta tcagatcatg gattctgtca cctgagaatt
      361 gagttatgaa agttactttt acagatttac ttacctgttc gaaaagtttt tgtgcatgct
      421 ttattttatc cagttcttct ttacgtaaac tttctttacc ttcgacattt tttgtttcaa
      481 taataaagtt aaggtagtca ccctttgttg tttttacaac ataagcaaag tcaggagaat
      541 atgtgtatcc acctgccacc ggaattttaa ttgagttttt cggtatcttt gtaaaaacag
      601 caacagagtg gatctctccg tctgttatat tgagtctttc aagctctgaa tcataaaaaa
      661 cttcttcaaa aagataagag tccagggggg cttttgtatt atcctgtaat actccaagat
      721 cacctgataa tacctcatgc aggtgattac cgtctgcatt agtgaatttt gtaggatgaa
      781 ttgagcctga aatcacatta tatccaaggc tgaatttgtt aaatgaattg ttcaacaaat
      841 acttgctaaa cccagattta atctttctga ttgtctgaat
//
LOCUS       DAZKGF010000455          878 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZKGF010000455 DAZKGF010000000
VERSION     DAZKGF010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.458"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>878
                     /locus_tag="ACHOQV_005322"
     rRNA            <1..>878
                     /locus_tag="ACHOQV_005322"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          205 a          198 c          279 g          196 t
ORIGIN      
        1 ccggtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa
       61 gttccgacct gcacgaatgg cgtaatgatg gccaggctgt ctccacccga gactcagtga
      121 aattgaactc gctgtgaaga tgcagtgtac ccgcggcaag acggaaagac cccgtgaacc
      181 tttactatag cttgacactg aacattgagc cttgatgtgt aggataggtg ggaggctttg
      241 aagtgtggac gccagtctgc atggagccga ccttgaaata ccacccttta atgtttgatg
      301 ttctaacgtt ggcccctgat cggggttgcg gacagtgtct ggtgggtagt ttgactgggg
      361 cggtctcctc ctaaagagta acggaggagc acgaaggttg gctaatcctg gtcggacatc
      421 aggaggttag tgcaatggca taagccagct tgactgcgag cgtgacggcg cgagcaggtg
      481 cgaaagcagg tcatagtgat ccggtggttc tgaatggaag ggccatcgct caacggataa
      541 aaggtactcc ggggataaca ggctgatacc gcccaagagt tcatatcgac ggcggtgttt
      601 ggcacctcga tgtcggctca tcacatcctg gggctgaagt aggtcccaag ggtatggctg
      661 ttcgccattt aaagtggtac gcgagctggg tttagaacgt cgtgagacag ttcggtccct
      721 atctgccgtg ggcgctggag aactgagggg ggctgctcct agtacgagag gaccggagtg
      781 gacgcatcac tggtgttcgg gttgtcatgc caatggcact gcccggtagc taaatgcgga
      841 agagataagt gctgaaagca tctaagcacg aaacttgc
//
LOCUS       DAZKGF010000456          877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZKGF010000456 DAZKGF010000000
VERSION     DAZKGF010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.459"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>877
                     /locus_tag="ACHOQV_005323"
     CDS             <1..>877
                     /locus_tag="ACHOQV_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9633520.1"
                     /translation="VPNNEAIVSVAQKSFGKEMAMIMFFAMVINIMIARFTPWKFIFL
                     TGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAMVFFPAIAHPYMKKVTGSDDV
                     AIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLFLRDTPVAISFTMSIIFLVTC
                     LFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQGVRMVIAEIVPAFKGISDKLV
                     PNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTLYLLNMIVIIPGVVPHFFVGA
                     AAGVFGNATGGRRGA"
BASE COUNT          182 a          194 c          235 g          266 t
ORIGIN      
        1 tagtgccgaa caatgaagcc attgtttccg tagcgcagaa aagcttcggg aaagaaatgg
       61 cgatgattat gttctttgcg atggttatca acattatgat tgcccgtttc acaccgtgga
      121 aatttatctt cctgaccggt catcacacgt tgtttatgtc gatgatggta gcggtaattc
      181 tggcaacagc aggcatgacc ggcattacgc ttatagccgt tggctctttg gttgttggcg
      241 tggcaatggt ctttttcccg gctattgcgc atccgtacat gaagaaagtg accggttctg
      301 atgacgttgc catcggccac ttctcaacat tatcttatgt attggccggt ttcatcggca
      361 gcaagtttgg taataaagag cactcgactg aagacatgaa tgtgccgaaa agtctgctgt
      421 tcttgcgcga cacgccggtt gctatctctt ttaccatgag cataattttc ctggtgacgt
      481 gcctgtttgc gggtgcagat gcggtgaaag agcttagcgg tggtaaaaac tggttcatgt
      541 tctctatcat gcaatccatt accttcgcag ctggcgtgta catcattctg cagggtgtgc
      601 gcatggtgat tgcggagatt gtcccggcat tcaaaggtat ttcagacaag ctggttccga
      661 atgccagacc tgctctcgat tgcccggtcg tcttccctta cgcacctaac gctgtactgg
      721 ttggtttcct gagtagcttt gctgcaggtt taatcggtat gttcacgttg tatctgctga
      781 acatgatcgt gattattcct ggtgtggtgc cgcacttctt cgtaggtgca gctgcagggg
      841 tattcggtaa tgcaacgggt ggacgtcgtg gtgcgat
//
LOCUS       DAZKGF010000457          872 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZKGF010000457 DAZKGF010000000
VERSION     DAZKGF010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.460"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>872
                     /locus_tag="ACHOQV_005324"
     CDS             <1..>872
                     /locus_tag="ACHOQV_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9633521.1"
                     /translation="FICSADAVPGLHHLPDKDEMRGVPPADALRAVQLLWNEMSNPGG
                     TFPVTHDTYLKLFQLSGADLSHRWDTILFDEAQDANPVTTALVLGQKCRVVLVGDCYQ
                     QIYRFRGANNALSHPALKNADRLWLTQSFRFGPAVARMANLLLQREGETREVKGCGGD
                     DEVLLKCHAREHLQGHYTVLSRTVAGVIGTALMAAMKGQKVYWVGGIEGYRTGELEDL
                     YWFQVDMPERMHSDRLRRDYRNFEEYKYIAKSTKDVEMNQSLRLLELCFPLPKKLELL
                     RQYTVTNEQDADITV"
BASE COUNT          195 a          186 c          276 g          215 t
ORIGIN      
        1 tttatctgtt ctgccgatgc ggtaccgggc ctgcatcacc tgccggataa ggatgagatg
       61 cggggtgttc cgccagcaga tgcgcttcgg gcggtgcagt tgctctggaa cgaaatgagc
      121 aaccccgggg ggacgttccc ggtgacgcac gatacttatc tgaaactctt ccagttgtcc
      181 ggtgcagacc tgtctcaccg atgggacacc atcctgtttg atgaggcgca ggatgcgaat
      241 ccggtgacca ctgctctggt tctggggcag aagtgtcggg tggtccttgt tggagattgt
      301 tatcaacaga tttaccgctt tcggggtgcg aataatgcgc tttcccatcc tgcactgaag
      361 aatgcggacc ggttgtggct gacacagagt ttccgttttg gtccggcggt ggctcgtatg
      421 gcaaatctgc ttctgcagcg tgaaggtgaa acacgagagg tgaaaggttg tggtggtgat
      481 gatgaggtat tgctgaaatg ccatgcgcgt gaacatctgc aggggcatta cacggtactc
      541 agtcgtactg tcgcgggtgt gattggtact gcgctgatgg cggcaatgaa agggcaaaag
      601 gtttactggg tggggggaat cgaaggttac aggaccgggg aacttgagga tctctactgg
      661 ttccaggttg atatgccgga acggatgcat tcagacaggc tgcgccggga ctacaggaat
      721 tttgaagaat ataaatatat tgcaaaatcc acgaaggatg tggagatgaa tcagtccctt
      781 cggcttctgg agttgtgttt tccgttaccg aaaaaactgg aacttctccg gcagtatacg
      841 gtaacgaatg agcaggatgc agatatcacg gt
//
LOCUS       DAZKGF010000458          870 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZKGF010000458 DAZKGF010000000
VERSION     DAZKGF010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.461"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            202..804
                     /gene="fimB"
                     /locus_tag="ACHOQV_005325"
     CDS             202..804
                     /gene="fimB"
                     /locus_tag="ACHOQV_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFP9633522.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEIQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          257 a          165 c          192 g          256 t
ORIGIN      
        1 gcatgcaaga gcatggcgtt tgtatggcaa agttattata agtaactatc gctactccat
       61 ttaagttcgc tcagaagaac tggtccactt acgttagtta ttaagcaaac gttcgctttt
      121 ataaacataa tcaggataaa aatgttggat tattgctaac ccagcacagc tagtgcgcgt
      181 ctgtaattat aagggaaaac gatgaagaat aaggctgata acaaaaaaag gaacttcctg
      241 acccatagtg aaatcgaatc actccttaaa gcagcaaata ccgggcctca tgcagcacgt
      301 aattattgtc tgactttgct ttgttttatt catggtttcc gggcgagtga aatttgccga
      361 ttgaggattt cggatattga tcttaaggca aagtgtatat atatccatcg attaaaaaaa
      421 ggcttttcaa caacgcaccc gctattgaac aaagaaattc aggctttaaa aaactggttg
      481 agtatccgta cttcttatcc gcatgctgag agcgagtggg tattcttatc acgtaagggg
      541 aatccacttt ctcggcaaca gttttaccat attatctcga cttccggtgg taatgccggg
      601 ttgtcactgg agattcatcc gcacatgtta cgccattcgt gtggttttgc tttggcgaat
      661 atgggaatag atacgcgact tatccaggat tatcttgggc atcgcaatat tcgtcatact
      721 gtctggtata ccgccagcaa tgcagggcgt ttttacggca tctgggatag agccagaggg
      781 agacagcgtc acgctgtttt atagagatag ttgagatacc agggatggtg ttaaaaacta
      841 cattattctt acgatatatc aaaaatgatt
//
LOCUS       DAZKGF010000459          865 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZKGF010000459 DAZKGF010000000
VERSION     DAZKGF010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.462"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(79..772)
                     /locus_tag="ACHOQV_005326"
                     /pseudo
     CDS             complement(79..772)
                     /locus_tag="ACHOQV_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          221 a          185 c          201 g          258 t
ORIGIN      
        1 tttcccctta tcgctgtcga ttctcattat cattcattgg taaaaaaaca gcaaaagagg
       61 ccctggtaat gactccagtc agtagtacat tgttttcgat aaatgaacca atggcttttc
      121 gtgaatttcc agggagcgtg aaaagcaaat cgttttccgg gctggtcgtt tgatgtgagt
      181 acgaagtgtc agattgttgc gctcaatgcg ccgggtaaat atcttgccga caagatgctt
      241 atcctgtggc atttctctgg tataactgct ccggttgtct ctggttatca tgcctgtgga
      301 gaatggcttc aggagttccg gcaactcacg acatgtttca tcagtgcgag gaccaaaagt
      361 gtaagccagc acacctccag atttggtgtt atacgcatac cagtgccact gctgacgagc
      421 tttgttttcg acaaaactcc attgttcatc aagttcgcag ataagcgtca catcggcact
      481 ggcgactgga gatgcggtta ctttgcgtgg tgagcgtttt ataaagtgag aatgacggtg
      541 ttgatatcca ccttcagcgt tctggagcta tcgcgaaccc cgactccgtt atgaaccatt
      601 tcaacaattt gctctttaat gtctggtttt cgggtttcgt agatgtaatt cagttgaaat
      661 acacacgttt gcaggattag cactggaaac gctcatgatc tgaggtgctg tgatcataat
      721 ggtacacttt ataggaatta cagcagggag aatgtactgt aactgttgcc atatggtctc
      781 cagataccaa tagaatacca cattaatcta tcgtcagaag gcatcaccgg ggccttctga
      841 cataatctgt taatacgtgt actat
//
LOCUS       DAZKGF010000460          848 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZKGF010000460 DAZKGF010000000
VERSION     DAZKGF010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.463"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHOQV_005327"
     CDS             complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHOQV_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP9633523.1"
                     /translation="MKIYRPLWSEGVLLSPQQFQQQTEWESFRSAGISALASPFPWGV
                     EKVELNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADHDAVTVV
                     IALPHMQPGVINVEQ"
     gene            complement(369..>848)
                     /locus_tag="ACHOQV_005328"
     CDS             complement(369..>848)
                     /locus_tag="ACHOQV_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP9633524.1"
                     /translation="PETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDA
                     TANSRINARLPYIFLLSRIAHYLKMIQRENIGTTKDRRLLELELNNWIRGLVTEMTDP
                     GDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
BASE COUNT          209 a          238 c          215 g          186 t
ORIGIN      
        1 tcctgctcaa cattaatcac gccaggctgc atgtggggaa gtgcaataac gacagttacc
       61 gcatcgtggt cagccagctg accggcatct aactctcgcg gtgccggagg caaatcgctc
      121 ctttgagcat caataagtgt tccgtcttcc agccagaggc gtaactgcgt aatctggatc
      181 agcccggatg acagaagact gtcattaagc tcaacctttt ccactcccca gggaaacggg
      241 gatgccagcg cagaaatacc ggcactgcga aaagactccc attcagtctg ctgctgaaac
      301 tgctgagggg agagcaaaac accttcgctc cacagcggac ggtaaatctt catttcccga
      361 cgcctgcctt acgcttttgc cttcggcatc tgcgaaacca gggacagact gacatccata
      421 ccttccacct ggaaatgcgg tacagcgtag agtttcacac ggaaaaatcc cgggttgtct
      481 tcgatatctt ccaccaccac ttttccgtca cgcagcggat gcgaggcctg caactcatca
      541 cccggatcgg tcatctccgt caccagaccc cggatccagt tattcagctc cagctcaagc
      601 agacggcgat ctttggtggt accgatattt tcccgctgaa tcatcttcag gtaatgcgca
      661 atgcgggaca gcaggaagat atagggaaga cgggcattaa tacggctgtt cgccgttgca
      721 tccggcgtat cgtacagtgc cggtttctgg gtggagttag cggagaagaa gcaggcataa
      781 tcccggttct tgtagtaaga gagcggaata aagcccagat tagcgaattc aaactcccgc
      841 gtttcagg
//
LOCUS       DAZKGF010000461          846 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZKGF010000461 DAZKGF010000000
VERSION     DAZKGF010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.464"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(146..445)
                     /locus_tag="ACHOQV_005329"
     CDS             complement(146..445)
                     /locus_tag="ACHOQV_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HFP9633525.1"
                     /translation="MNKMFSQWIDFDEMITPKIIKVIYWFFLFITVAGGVIGMIANPF
                     VGLLGLIMGIIGLRIGCEMIILLFNIYKQVKKIADAVPDKINEQAEDSLLSQNKH"
BASE COUNT          349 a          169 c          112 g          216 t
ORIGIN      
        1 aagcgacaga taatgctttc tgagcatatc agagcgtata aaaggcgtca atcatcatca
       61 ctcagaacat aaccaggaag aacagaaaac agtctgcaga aaaaataaca gggacagtat
      121 taacagtccc tgttattacc attcatcaat gtttattctg agataataaa ctatcctcag
      181 cctgttcgtt tattttatcc ggtaccgcat cagcaatctt tttaacctgc ttataaatat
      241 taaaaagaag aattatcatt tcacacccaa tcctcagacc aataataccc ataataagac
      301 caagcagtcc gacaaacgga ttggctatca tacctataac accaccggcc actgtaataa
      361 aaagaaaaaa ccagtatatt accttgatga tcttcggagt aatcatctca tcaaaatcaa
      421 tccactgact gaacatttta ttcattttca cctctccagc aatcggttgt tagcagcaat
      481 gtaaaaataa aaacactgct aacgcgtaaa atacaaacag ataaagaatg tgtcaatatg
      541 ttacatgtac taaaacatca gaattatgtt tagcatataa acaatcacaa gatagttgta
      601 atagcaagaa acaacaaaca gagaaagaaa aagcgaacaa aataaaacat ttaaagcctt
      661 aataaaaata accttcccaa aatatactta caagaaagta taatatcgga caatatttat
      721 cattaacagt tcacctaaac atctataaga atatcaaagg tatcatcata aaatgacata
      781 cagaaaaaca acatcccctg gcagtatgta tgccagggga tatattatat agtacacgtt
      841 acctga
//
LOCUS       DAZKGF010000462          838 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZKGF010000462 DAZKGF010000000
VERSION     DAZKGF010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.465"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            241..624
                     /locus_tag="ACHOQV_005330"
     CDS             241..624
                     /locus_tag="ACHOQV_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633526.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            618..>838
                     /locus_tag="ACHOQV_005331"
     CDS             618..>838
                     /locus_tag="ACHOQV_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9633527.1"
                     /translation="MVILASLIFVINLTVRCTLTVQGACIFFRGHALMKTKKHTSRTA
                     TKTPERDLYQLVTDRVVTAIENGVPPWKRP"
BASE COUNT          185 a          192 c          234 g          227 t
ORIGIN      
        1 ttgacagctt actgctgcag tttttgtccg gtcaggggcg acagttatcc ccggatgact
       61 gggcagcact ggaagaggcc gaatatcagc tttactggct gcgggctctt gccggtgggc
      121 ggtaacagaa cgccgtagtt tctttatccg gaataacgtc atcacgtatt ccgtcattat
      181 atcacccggc agggacatat ccctgtgggg agagtcccgg ctttattcag gagaattgtt
      241 atgggactcg atatttactt tctggctcgt gataaccatc acagtggtgc cgttgtggga
      301 acacaggaac atcaggaggt gggggctttc cgtaaggtga aaccgcttct gacatggttt
      361 cgtgaacata ccggtgcttc agatgagcag catgctttcc ctgtaacccg cagttcactg
      421 gctgcattat tgcgcgacct cagtatgctg acaccgggga actgccacag gctgtttcct
      481 tatgtcacag gctcgtgttt acggggggaa gattatgccg gtgattactg gtatgacgtg
      541 gatgacgtaa aagcgtgggt cagcaatacg ctgaagacgt ttgattttga gcgtcgttca
      601 ctggtgatgc tggcgttgtg gtaattctgg ccagtctgat tttcgttatt aatttaactg
      661 tacggtgcac tctcaccgta cagggagcgt gcatcttttt cagggggcat gctcttatga
      721 aaacgaaaaa acatacttca cgtaccgcga ctaaaactcc ggaacgtgac ctgtatcagc
      781 ttgtgaccga cagggttgtt acggccattg aaaatggtgt gccaccctgg aaacgtcc
//
LOCUS       DAZKGF010000463          835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZKGF010000463 DAZKGF010000000
VERSION     DAZKGF010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.466"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..363
                     /locus_tag="ACHOQV_005332"
     CDS             <1..363
                     /locus_tag="ACHOQV_005332"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633528.1"
                     /translation="FDPETMSREDMLREIQLLRDSRDLALELVFGYPETKGAEQIELN
                     DDEITLLKAKLPEHHFQWLAGDGDEALTVGRLGYFADLADDFSVLAGEIDPDLEEPEE
                     EVISRGPCGVVFCLIESD"
     gene            633..>835
                     /locus_tag="ACHOQV_005333"
     CDS             633..>835
                     /locus_tag="ACHOQV_005333"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633529.1"
                     /translation="MGDWHTKPRTGASQFAEYEEEIRLLKEKQYTNKQIYEAISQKGI
                     TIGQQMFNIYMRRMFNGETYIKT"
BASE COUNT          274 a          147 c          215 g          199 t
ORIGIN      
        1 ttcgatccag aaaccatgag cagggaggat atgcttaggg aaattcagct actgcgcgac
       61 tcacgtgatc tggcgcttga actggtgttc ggctatccag aaacaaaggg cgcagaacaa
      121 attgaattga atgatgatga aataaccctt ctaaaagcga aactaccgga acaccatttt
      181 caatggttag caggagatgg agatgaggcg ctaacagttg ggcgattagg ttattttgca
      241 gatctggccg acgattttag cgttctggcc ggagagatag atccagacct ggaagaacca
      301 gaggaagaag tgatatcccg tgggccttgt ggtgtcgtat tttgtttgat tgagtctgac
      361 tgatcttgtg tgtgaggtgt agtaaatgcc gcgtctgcgg catgggaggc gacaaaaaca
      421 agttaaatat aaattggatg taaagtgtcg ccgtgacgcg gctacgccgc tcggatgtaa
      481 ataaatcaca ggaacacctc cccaccctgc cagacgtgtt tgtgtctggc gcagaatgcg
      541 ccagacaacc atatatcagg caacacgtta cttaatggtc gtagacaagc aattgtaaca
      601 gtaacaggct tgaattttat ggagatgatg aaatgggaga ttggcatact aagccaagaa
      661 caggagcttc tcaatttgct gagtatgaag aggaaataag attattaaag gaaaaacaat
      721 atacgaacaa acaaatatat gaggcaatat cccagaaagg gataacaata ggacagcaaa
      781 tgtttaatat atatatgagg cgtatgttta atggcgaaac atatataaaa acaga
//
LOCUS       DAZKGF010000464          829 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZKGF010000464 DAZKGF010000000
VERSION     DAZKGF010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.467"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>829
                     /locus_tag="ACHOQV_005334"
     CDS             <1..>829
                     /locus_tag="ACHOQV_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFP9633530.1"
                     /translation="ALHSSWLETLSCWFENKRIIAFCDETQTFSFEKGTNYADLQRII
                     NPQFSFNLSVVLRCPRAVTDYLIAVSPASYQITSPRNNEPDTLKELVVTDPLSTLLNE
                     ILQLKESSVLPDEITVLVPTDLVKNSLQNDMRTLGVNIETVSRFRGMESSVVITLYSE
                     SMEDAQLFCAYSRATTAFIAIYDSEKLAWREKNKFLHKLVNRKEVQRAINEAKHNSSS
                     SVLMAGFFREGELKLSTVKLAWSKHFSSWLVEFDERNSPEEMWIDYLYSYLPYPIIY"
BASE COUNT          254 a          138 c          161 g          276 t
ORIGIN      
        1 aggcgttaca ttcttcgtgg ctggagactt tatcttgttg gttcgaaaat aaaaggatta
       61 tcgcattttg tgatgaaact caaacatttt cctttgaaaa aggaacaaat tacgctgatc
      121 ttcagcgaat aataaatccc caattttctt ttaacttatc tgttgtgtta agatgtccta
      181 gagccgttac tgattattta atagctgtca gccctgcctc ataccaaatt acttccccta
      241 ggaataacga accggatacg ctaaaagaat tagttgttac agatccactc tccacattgc
      301 ttaatgaaat tttacaattg aaagaaagtt ctgtactgcc tgatgagatt actgtccttg
      361 ttcctacgga tttagtgaaa aattccctcc agaatgatat gagaactttg ggggtgaata
      421 ttgaaacagt atctcgtttt cgaggtatgg aatcatctgt cgtgattaca ttgtattccg
      481 aaagcatgga ggatgcacag ttattttgtg cttattcaag agctacaact gcttttattg
      541 caatttatga ttcggagaaa ttagcatgga gagaaaaaaa taaatttttg cataaactgg
      601 ttaacagaaa ggaggttcag agagcaatta atgaagcaaa gcataattct tcctccagtg
      661 ttcttatggc cggttttttc cgcgaaggag agttaaaact tagtactgta aaattagcat
      721 ggtcgaagca cttttcttcg tggcttgttg aatttgatga aagaaattct ccagaagaaa
      781 tgtggataga ttatttgtat tcctatttgc cttacccgat tatatattg
//
LOCUS       DAZKGF010000465          808 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZKGF010000465 DAZKGF010000000
VERSION     DAZKGF010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.468"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>808)
                     /locus_tag="ACHOQV_005335"
     rRNA            complement(<1..>808)
                     /locus_tag="ACHOQV_005335"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          157 a          256 c          170 g          225 t
ORIGIN      
        1 tgctgtgttt ttaataaaca gttgcagcca gctggtatct tcgactgatt tcagctccac
       61 gagcaagtcg cttcacctac atatcagcgt gccttctccc gaagttacgg caccattttg
      121 cctagttcct tcacccgagt tctctcaagc gccttggtat tctctacctg accacctgtg
      181 tcggtttggg gtacgatttg atgttacctg atgcttagag gcttttcctg gaagcagggc
      241 atttgttgct tcagcaccgt agtgcctcgt catcacgcct cagccttgat tttccggatt
      301 tgcctggaaa accagcctac acgcttaaac cgggacaacc gtcgcccggc caacatagcc
      361 ttctccgtcc ccccttcgca gtaacaccaa gtacaggaat attaacctgt ttcccatcga
      421 ctacgccttt cggcctcgcc ttaggggtcg actcaccctg ccccgattaa cgttggacag
      481 gaacccttgg tcttccggcg agcgggcttt tcacccgctt tatcgttact tatgtcagca
      541 ttcgcacttc tgatacctcc agcatacctc acagtacacc ttcacaggct tacagaacgc
      601 tcccctaccc aacaacactt agtgtcgctg ccgcagcttc ggtgcatggt ttagccccgt
      661 tacatcttcc gcgcaggccg actcgaccag tgagctatta cgctttcttt aaatgatggc
      721 tgcttctaag ccaacatcct ggctgtctgg gccttcccac atcgtttccc acttaaccat
      781 gactttggga ccttagctgg cggtctgg
//
LOCUS       DAZKGF010000466          804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZKGF010000466 DAZKGF010000000
VERSION     DAZKGF010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.469"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            230..>804
                     /locus_tag="ACHOQV_005336"
     CDS             230..>804
                     /locus_tag="ACHOQV_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633531.1"
                     /translation="MSPDIISSAVNRFSGWPSNDPLKTDKESMTSPMTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNLDEEGHYLAFG
                     YNVDTPDRITVSAYNEKKPGCYGQCLLIQDDETDLFNLIKVKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEP"
BASE COUNT          251 a          162 c          153 g          238 t
ORIGIN      
        1 gattgttgcg tttatgagca ccggccgtaa gcccattatg ttattgtttc attagtaata
       61 atcacttaaa tgtatcgatg aaacaggtat gacttagcgg gagtgattgt ttctgagtga
      121 acagagacca ctttcgcctg ataaatgaaa aatgagcgat ttttgcaagt tagtttttct
      181 atagccgagt acgctcagcg cctcgcttga ttacaatgag gttgctgcta tgagtcctga
      241 tattatcagt tctgctgtca atcgcttctc aggttggcca tctaatgatc ctctaaaaac
      301 agataaagaa tcaatgacat cacccatgac tgtgcaggta ataaagatga ttgccgccat
      361 ccttgaccca catcggatag tcgttggaat cgatacactt tatacaacgc agcgcgaaac
      421 atttacctca gagagtcaac agctcattga atcagcattg caaacacttc atgtgatgaa
      481 tttaaataac ctggaaacaa atctcgatga ggaaggtcac tatctggctt ttggatataa
      541 cgttgacact cctgacagaa taaccgtttc tgcttataat gaaaaaaagc ctggctgcta
      601 tggccaatgt cttttaatac aggatgacga aacagactta tttaacctga taaaagttaa
      661 aatttttgaa gccaggcata attatgtcgc tcaattgatg agcgatccgg aatttatgtt
      721 caaatttagt tatagcgctc aacaaaatag attagaacct catcttctgg ttcccactta
      781 tttccctcta aaaacaaacg aacc
//
LOCUS       DAZKGF010000467          802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZKGF010000467 DAZKGF010000000
VERSION     DAZKGF010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.470"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>802
                     /locus_tag="ACHOQV_005337"
     CDS             <1..>802
                     /locus_tag="ACHOQV_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
                     /protein_id="HFP9633532.1"
                     /translation="GTTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVE
                     KSGSGTLTVSNTTLIQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAID
                     PTNVTLTSGATWNIPDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNG
                     TISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVIEAING
                     ATTEEGAFVQGNMLQAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASM"
BASE COUNT          202 a          234 c          226 g          140 t
ORIGIN      
        1 gggcaccacc acgctgaata acggtgccac gcttaccctt tccgggaaaa cggtgaataa
       61 cgataccctg accatccatg aaggtgatgc actcctgcag ggaggcgctc ttaccggtaa
      121 cggcagggtg gaaaaatcag gaagtggcac actcactgtc agcaacacca cactcatcca
      181 gaaaaccgtc aacctgaatg aaggcacgct gacgctgaac gacagtaccg tcaccacgga
      241 tgtcatcgct cagcgcggca cggccctgaa gctgaccggc agcacagtgc tgaacggtgc
      301 cattgacccc acgaatgtca ccctgacatc cggtgccacc tggaatatcc ccgataacgc
      361 cacggttcag tcggtggtgg atgacctcag ccatgccgga cagattcatt tcacctccgc
      421 ccgcacaggg aagttcgttc cgacaaccct gcaggtgaaa aacctgaacg ggcagaatgg
      481 caccatcagc ctgcgtgtac gcccggatat ggcgcagaac aatgctgaca gactggtcat
      541 tgacggtggc agggcaaccg gaaaaaccat cctgaatctg gtgaacgccg gcaacagtgg
      601 tacggggctg gcgaccaccg gtaagggtat tcaggtgatt gaggccatta acggtgccac
      661 cacggaggaa ggggcttttg tccaggggaa tatgctgcag gccggggcct ttaactacac
      721 cctcaaccgg gacagtgatg agagctggta tctgcgcagt gaagaacgtt atcgtgctga
      781 agtccccctg tacgcctcca tg
//
LOCUS       DAZKGF010000468          796 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZKGF010000468 DAZKGF010000000
VERSION     DAZKGF010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.471"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..178)
                     /locus_tag="ACHOQV_005338"
     CDS             complement(<1..178)
                     /locus_tag="ACHOQV_005338"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFP9633533.1"
                     /translation="MIGKAIIKAQYNKQVPLTLVAMSVLTAMSIACQNQVDVCSPGNL
                     RGAVNIYTMVLEKGK"
     gene            complement(405..701)
                     /locus_tag="ACHOQV_005339"
     CDS             complement(405..701)
                     /locus_tag="ACHOQV_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9633534.1"
                     /translation="MPDQSGYCWTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
BASE COUNT          220 a          170 c          161 g          245 t
ORIGIN      
        1 ttttcccttt ctccagaacc atagtgtaaa tattaacggc cccacgtaaa tttccggggc
       61 tgcatacgtc aacctgattc tgacaggcaa ttgacatcgc tgttaatact gacattgcaa
      121 ccagagtcag aggtacttgt ttattgtatt gagctttaat tattgccttc cctatcattg
      181 atggtcacgc atatactgaa tacggcatat ccggtttgat gtaagacata aatgcttcct
      241 ttttcgtaat acgttttggt tatagcagca attaccgcta caatagcgac aatcgctgct
      301 ctttcaacaa tgaatgctgg aaattctgtt tatcaattaa acttctggct aaattgttct
      361 tatgcttctt tttcctcctg aggtgatcac gtgatgacaa aaacttatct gctcatggtc
      421 atatctgaag aaagcgtatg tcgcacttcg tcattaacac ctttgactgc tgatctggca
      481 ggtacagtat gtaaagtaag ccattcgtca atagctgatg aacgccagcc cacagaacca
      541 ctaccaagac gtaccggcct ggggaaggtt gcatcgtagt atttcgataa cggattcatt
      601 ttttcgtaaa ttgtcgaacg agatatacct aacaattggc tcagttcagg catccgtaag
      661 atgcgtgaag gagtccagca gtatccggat tgatctggca ttttatcccc catagtgtgg
      721 tgaactgtcg ttacagtttg tattccccgg gtaaatttgt ctgcgtaaaa aaatgggctg
      781 agcaaaaaga caataa
//
LOCUS       DAZKGF010000469          785 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZKGF010000469 DAZKGF010000000
VERSION     DAZKGF010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.472"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>785
                     /locus_tag="ACHOQV_005340"
     CDS             <1..>785
                     /locus_tag="ACHOQV_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP9633535.1"
                     /translation="LWLVRGGKATQPDGHTLARLWASLPPDIRLSPHLYLATSSAQGP
                     WWILGWSERVPGAEDVLPAPLPPYRMLTGLADRFGQTLTYRREAAGDLAGEITGVTDG
                     AGREFRLVLTTQAQRAEEARTSSLSSSDRPRPLSVSAFPDTLPGTEYGPDRGIRLSAV
                     WLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRIVGHRYA
                     GRPEMRYRYDDAGRVVEQLNPTGLSYRYQYEQDRITITDSLNRR"
BASE COUNT          130 a          251 c          277 g          127 t
ORIGIN      
        1 ctgtggctgg tgcgtggcgg taaggcaacg cagccggacg gccacacgct ggcacggctg
       61 tgggcgtcgc tgccgccgga tatccggtta agcccgcatc tttacctggc gaccagcagc
      121 gcacaggggc cgtggtggat actggggtgg tctgagcggg tgccgggtgc tgaggacgta
      181 ctgccagcgc cgctgccgcc gtaccggatg cttaccgggc tggcggaccg cttcgggcag
      241 acgctgacgt accggcgtga ggccgccggt gacctggccg gggaaatcac cggcgtgacg
      301 gacggtgccg ggcgtgagtt ccgtctggtg ctgaccacgc aggcgcagcg tgcggaagag
      361 gcccgcacct cttcgctatc ttcttctgac cgtccccgcc ctctctcagt ctcagcgttc
      421 cccgacacac tgcccggtac cgaatacggc cccgacaggg gtatccgcct ttcggcggtg
      481 tggctgatgc acgacccggc atacccggaa agcctgcccg gtgcgccact ggcgcggtac
      541 acgtatacgg aagccggtga actgctggcg gtatatgacc gcagcaatac gcaggtgcgc
      601 gctttcacgt atgacgcgca gcacccgggc cggatagtgg ggcaccgtta cgcgggaagg
      661 ccggagatgc gctaccgcta cgacgatgcg gggcgggtgg tggagcagct gaacccgaca
      721 ggcctgagtt accgctacca gtatgagcag gaccgcatca ctatcacgga cagcctgaac
      781 cggcg
//
LOCUS       DAZKGF010000470          779 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZKGF010000470 DAZKGF010000000
VERSION     DAZKGF010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.473"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            16..216
                     /locus_tag="ACHOQV_005341"
                     /pseudo
     CDS             16..216
                     /locus_tag="ACHOQV_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
BASE COUNT          252 a          166 c          147 g          214 t
ORIGIN      
        1 atttgaggta ttgtcatgac aaataaacac ctatcactgt tttctccttt tatgctgaca
       61 gaaaaaataa agccatgtaa tcgtatcgtt atggctccta tgacgacctg gtcagctaat
      121 cctgatggaa ccatctctga gcaagaactc gaatattata aacgtcgctc acaaaatatt
      181 ggtttagtaa ttacaggata tacctatgtc acgccaagga aattcagaca tcgaaaggaa
      241 ctggctggtt tccgttaatg gattaaatat cgattactct cccgagagta aaactatcaa
      301 atcaatgagt aaccgataca gcctcgcaaa ttctttcaga taattaaccc gtcactgcgg
      361 cacgttctct cgtaaggcgt gcctgcagtg taatccagta tgtatccggc atagttgacg
      421 gtttaataga ctcaattcct ctccctggtt acctacaacg atatcttgta taacacccca
      481 ggagatacga cgttacgatg agatgcaata tgctcctcag cgccaatcaa cgccaggact
      541 gggccagtct tgagatcaga ctcattcccg taactgattt caggcgacat atatcaatga
      601 cgttggggca ataaaagata gccgatgtaa tacatctatt caaatttgtg atacatgtca
      661 aaaaattggt tgaccaaact cgttatttat ataagggcac ttacgaagtg cactcttttt
      721 taaagcgagg aagtaccaat gaaagagaat aaagtacagc aaatcagtca taaactgat
//
LOCUS       DAZKGF010000471          770 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZKGF010000471 DAZKGF010000000
VERSION     DAZKGF010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.474"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>770
                     /locus_tag="ACHOQV_005342"
     CDS             <1..>770
                     /locus_tag="ACHOQV_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP9633536.1"
                     /translation="HGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTINKNGRQ
                     VIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGWQVIKE
                     GGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSVVAGKA
                     DNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADSGAAVS
                     GTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDT"
BASE COUNT          188 a          199 c          252 g          131 t
ORIGIN      
        1 acatggcgga ctggcaaccg gaaccatcgt caacaccggt gcagaaggtg gtccggagtc
       61 tgaaaatgtg tccagcggtc agatggtcgg agggacggct gaatccacca ccatcaacaa
      121 aaatggccgg caggttatct ggtcttcggg gatggcacgg gacaccctca tttacgccgg
      181 tggtgaccag acggtacacg gagaggcaca taacacccgg ctggaggggg gtaaccagta
      241 tgtacacaac ggtggcacgg caacagagac gctgataaac cgtgatggct ggcaggtgat
      301 taaggaagga ggaactgccg cgcataccac catcaaccag aaaggaaagc tgcaggtgaa
      361 tgccggcggt aaagcgtctg atgtcaccca gaacacgggc ggtgcactgg ttaccagtac
      421 agctgcaacc gtcaccggta caaaccgcct gggagcattc tccgttgtgg cgggtaaggc
      481 tgataatgtc gtactggaaa atggcggacg tctggatgtg ctgagcggac acacagccac
      541 taatacccgt gtggatgatg gcggaacgct ggatgtccgc aacggtggtg ccgccaccac
      601 cgtatccatg gggaatggcg gtgtactgct ggccgattcc ggtgccgctg tcagtggtac
      661 ccggagcgac ggaacggcat tccgtatcgg aggcggtcag gcggatgccc tgatgctgga
      721 aaaaggcagt tcattcacgc tgaacgccgg tgatacggcc acggatacca
//
LOCUS       DAZKGF010000472          758 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZKGF010000472 DAZKGF010000000
VERSION     DAZKGF010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.475"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(151..525)
                     /locus_tag="ACHOQV_005343"
     CDS             complement(151..525)
                     /locus_tag="ACHOQV_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633537.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDLTVVDAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
BASE COUNT          207 a          187 c          192 g          172 t
ORIGIN      
        1 taaatcgcac tctggcgcaa tgttcactat cggggcgagc gttcaccgga aaacgtcata
       61 caggcttaca ggcggcttaa aattcgatgg acggaggacc ggatgggaat cgggagacct
      121 ggacaaaaag tgcaggcctc catgacacgg tcaggacagt ttgtgtttaa ccagaatatt
      181 tagcgcctgt atcggggctt cgtggagttc ccgcagacat tcagggtcag ttacatgttc
      241 ctggtatctt gttttctcaa gtaacaagcg gtgcatgtgc agcatacggg caagagccgc
      301 gcacacctcc tcaaatgcgg tatcccgctt accgtcactg tcagagccac aaaacagcgg
      361 cacaacctcc acgccgtcgc tgtgggcctt tagccaggca tcaaccacgg ttaaatcatc
      421 ggtgaagaac cacgcactgt tagcgctgaa tcgcattttc caggcttccg gtactggttg
      481 cggggagtat gaatatgctg gtggtatctt gtcgtgagta tccataggct atcgttccgt
      541 cattctgtta ttcaccacgg cgctaaaaac gccatgaagg accagaatac cgattgcctc
      601 gtttgctgta tgaggtgctt accacacatt gggtacacag atgaatcagg tggaagtgtg
      661 attaccggaa aaaaaacctt tcagtttgac gaaaaaaaaa ccgcagtgtg ataacactac
      721 ggcacgccac caaagaaaca taacaaggaa tcaacaat
//
LOCUS       DAZKGF010000473          757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZKGF010000473 DAZKGF010000000
VERSION     DAZKGF010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.476"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>757)
                     /locus_tag="ACHOQV_005344"
     CDS             complement(<1..>757)
                     /locus_tag="ACHOQV_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFP9633538.1"
                     /translation="FALQFGITLTGKHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEV
                     FWIPKFTRLHEVASAMATHDKYRTTYFSTPSAKTHQAYLVWNGDDWRGDDPARRAVEF
                     PKESAMRVGCECPDGIWRYIIRLEEAVAGGLSARVDIERIRNRYNPTTYAMLYGCEFV
                     DSKDAVFKFSELVRCEVEMETWGDYDPTAARPFGNREVWAGFDPSRSGDNSTFVIVAP
                     PVHEGERFRVLAVWQWQGFNFTWQADQIRELMQR"
BASE COUNT          168 a          239 c          200 g          150 t
ORIGIN      
        1 cgctgcatca gttcgcggat ctgatcggcc tgccaggtga agttaaatcc ctgccactgc
       61 cagacggcga gcacacggaa ccgctcgccc tcatggacag gcggagccac aattacaaac
      121 gtggaattat ccccggacct tgacgggtca aagcccgccc acacctcacg attgccgaac
      181 ggacgtgccg ccgtgggatc gtagtcaccc cacgtctcca tctccacctc gcacctgacc
      241 agttcggaga acttaaatac cgcatccttg ctgtcaacaa actcgcagcc gtacagcatc
      301 gcgtaagtgg tcggattgta gcggttcctg atacgttcaa tatcaacccg agcagacagg
      361 ccacccgcca ccgcctcttc cagccggata atgtagcgcc agatcccgtc agggcattca
      421 cagcccacac gcatcgcgct ttctttcgga aactcaacag cacgacgcgc cgggtcatcc
      481 ccgcgccagt cgtcaccatt ccagaccaga taagcctggt gggttttggc gctgggtgtg
      541 gaaaaatacg tcgtgcggta tttgtcgtgg gtcgccattg ccgaggccac ttcatgcagt
      601 cgcgtgaatt ttgggatcca gaacacctca tcgccgtaaa ggtggccgtt aaatccctgg
      661 gcggtactgg cattggtgga caggaaacgc agcattgcac cattgctcag acggatatgc
      721 ttacccgtga gcgtgatccc gaactgcagg gcgaagt
//
LOCUS       DAZKGF010000474          757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZKGF010000474 DAZKGF010000000
VERSION     DAZKGF010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.477"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>757)
                     /locus_tag="ACHOQV_005345"
     CDS             complement(<1..>757)
                     /locus_tag="ACHOQV_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFP9633539.1"
                     /translation="FAQQFGITLTGKHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEV
                     FWIPKFTRLHEVASAMATHDKYRTTYFSTPSAKTHQAYLVWNGDDWRGDDPTRRAVEF
                     PKESAMRVGCECPDGIWRYIIRLEEAVAGGLSARVDIERIRNLYNPTTYAMLYGCEFV
                     DSKDAVFKFSELVRCEVEMETWGDYDPTAARPFGNREVWAGFDPSRSGDNSTFVIVAP
                     PVHEGERFRVLAVWQWQGFNFTWQADQIRELMQR"
BASE COUNT          165 a          235 c          202 g          155 t
ORIGIN      
        1 cgctgcatca gttcgcggat ctgatcggcc tgccaggtga agttaaatcc ctgccactgc
       61 cagacggcga gcacacggaa ccgctcgcct tcatggacag gtggagccac aattacaaac
      121 gtggaattat ccccggacct tgacgggtca aagcccgccc acacctcacg attgccgaac
      181 ggacgtgccg ccgtgggatc gtagtcaccc cacgtctcca tctccacctc gcacctgacc
      241 agttcggaga acttaaatac cgcatccttg ctgtcaacaa actcgcagcc gtacagcatc
      301 gcataggtgg tcgggttgta gaggttcctg atacgttcaa tatcaacccg tgcagacagg
      361 ccacccgcca ccgcctcttc cagccggata atgtagcgcc agatcccgtc agggcattca
      421 cagcccacac gcatcgcgct ttctttcgga aactcaacag cacgacgcgt cgggtcatcc
      481 ccgcgccagt cgtcaccgtt ccagaccaga taagcctggt gggttttggc gctgggtgtg
      541 gaaaaatacg tcgtgcggta tttgtcgtgg gtcgccattg ccgaggccac ttcatgcagt
      601 cgcgtgaatt ttgggatcca gaacacctca tcgccgtaaa ggtggccgtt aaatccctgg
      661 gcggtactgg cattggtgga caggaagcgc agcattgcac cattgctcag acggatatgc
      721 ttacccgtga gcgtgatccc gaactgctgg gcgaaat
//
LOCUS       DAZKGF010000475          753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZKGF010000475 DAZKGF010000000
VERSION     DAZKGF010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.478"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..>753
                     /locus_tag="ACHOQV_005346"
     CDS             38..>753
                     /locus_tag="ACHOQV_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9633540.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          251 a          132 c          164 g          206 t
ORIGIN      
        1 ccagtaatgt aaataaattt tcgaggagat cattccagtg ggacgtaaat gggccaatat
       61 tgttgctaaa aaaacggcta aagacggtgc aacgtctaaa atttatgcaa aattcggtgt
      121 agaaatctat gctgctgcca aacaaggtga acccgatcca gaattaaaca catctttaaa
      181 attcgttatt gaacgtgcaa agcaggcaca agttccaaag cacgttattg ataaagcaat
      241 tgataaagcc aaaggcggcg gagatgaaac gttcgtacag ggacgttatg aaggctttgg
      301 tcctaatggc tcaatgatta ttgctgagac attgacttca aatgttaacc gtacgattgc
      361 taacgttcgc acaattttca ataaaaaagg cggcaatatc ggagcggcag gttctgtcag
      421 ctatatgttt gacaatacgg gtgtgattgt atttaaagga acagaccctg accatatttt
      481 tgaaatttta cttgaagctg aagttgatgt tcgtgatgtg actgaagaag aaggtaacat
      541 tgttatttat actgaaccta ccgaccttca taaaggaatc gcggctctaa aagcagctgg
      601 aatcactgag ttctcaacaa cagaattaga aatgattgct caatctgaag ttgagctttc
      661 cccagaagat ttagaaatct ttgaagggct tgttgatgcc cttgaagatg acgacgatgt
      721 acaaaaagtt tatcataacg tcgcaaatct cta
//
LOCUS       DAZKGF010000476          753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZKGF010000476 DAZKGF010000000
VERSION     DAZKGF010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.479"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>753
                     /locus_tag="ACHOQV_005347"
     CDS             <1..>753
                     /locus_tag="ACHOQV_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9633541.1"
                     /translation="ARECELTGELLESRSDISRQSETLHLQQALHDIQQNQPLLSVDV
                     DVRTVAGVVADWTGVPLSSLMKDEQTELLHLEKDIGRRVVGQDVALESIAQRLRAAKT
                     GLTSGNGPQGVFLLVGPSGVGKTETALALADVMYGGEKSLITINLSEYQEPHTVSQLK
                     GSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGEGR
                     EIDFRNTVILMTSNLGSDLLMQQLSEKPETTES"
BASE COUNT          169 a          189 c          243 g          152 t
ORIGIN      
        1 gcccgggagt gcgaactcac cggtgagtta cttgaaagtc gcagcgatat ttcccgtcag
       61 agtgagacac tgcatctgca gcaggcactg cacgacattc agcagaatca gcccctgctc
      121 agtgtggatg ttgacgtacg caccgttgcc ggtgtggtcg cggactggac cggggtgccg
      181 ttatcttccc tgatgaagga cgaacagacg gaactgctgc atctggaaaa ggatatcggc
      241 agacgggtgg tcggacagga cgtggcactg gaatccattg cgcagcgtct gcgtgcggcg
      301 aaaacgggtc tgacatccgg taacggcccc cagggggtgt tcctgctggt tgggcccagt
      361 ggtgtgggaa aaaccgaaac ggcactggcg ctggcagacg tgatgtacgg cggcgaaaaa
      421 tcactgatta ccatcaatct gtcggaatac caggagcccc atacggtttc ccagcttaag
      481 ggttcaccgc ccgggtacgt gggatacggt cagggcggta ttctgacgga agctgtgcgt
      541 aaacgccctt acagcgtggt gctgctggat gaagtggaaa aggcccaccg ggatgtgctg
      601 aatctgttct accaggtgtt tgaccggggc tttatgcgcg acggcgaggg acgtgaaatt
      661 gacttccgta atacggtcat tctgatgacc tccaacctgg gtagtgacct tctgatgcag
      721 caactgagcg aaaagccgga gacaacggaa tcg
//
LOCUS       DAZKGF010000477          736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZKGF010000477 DAZKGF010000000
VERSION     DAZKGF010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.480"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..>736
                     /locus_tag="ACHOQV_005348"
     CDS             33..>736
                     /locus_tag="ACHOQV_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9633542.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDAAQFGGADTDWSTVD
                     YPRLTDMDDNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDIEAQ
                     GASNQTDFNINLTECSNTEDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATG
                     VGIRIYSKGTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTV
                     APGVVKSTASFVVLYE"
BASE COUNT          208 a          160 c          187 g          181 t
ORIGIN      
        1 gcttggattt tttaacaaaa ggaaaggtat aaatgaaatt caatttatct aatttatccg
       61 cagtattact ggcatcaggt atgctgatgt ctactgcggt aaccgcagca cccggcgatg
      121 cagcacaatt tggtggagcg gatactgact ggagcaccgt tgattatccc aggctcactg
      181 atatggatga caacgttgat tcaatggggg ggaaaatccg ctttactggc cgtgtagtga
      241 aagctacctg taaggtcgca accgattcaa aacagattga agttgtcctg ccggttgtgc
      301 cttccaacct tttcactggt atcgacatag aagcacaggg ggcgagcaac cagaccgatt
      361 tcaatattaa tctgaccgaa tgtagcaata cagaggatca gaaaattgag ttccgcttta
      421 ccggtaccgc agatagcgct aataaaacgc tcgctaacga agtagaagga tcaacggatg
      481 ctgacaacag cggcaatgcg ggggcgactg gtgtagggat tcgaatttac tccaaaggta
      541 cgacgaataa tggtctgatt aacctgaata ctactgcggc agagggtagc gcctccaccg
      601 ccgcttatac aattccagga aatgctacga cccatgattt cagcgcggcc tttactgcag
      661 gttatgctca aaacggtagc actgttgcac ctggtgtagt taagtcaaca gcaagttttg
      721 tggtgctgta cgagta
//
LOCUS       DAZKGF010000478          733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZKGF010000478 DAZKGF010000000
VERSION     DAZKGF010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.481"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(10..681)
                     /gene="queE"
                     /locus_tag="ACHOQV_005349"
     CDS             complement(10..681)
                     /gene="queE"
                     /locus_tag="ACHOQV_005349"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFP9633543.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          175 a          198 c          168 g          192 t
ORIGIN      
        1 aatgtttaat caggcaatat ttagatattt atgtgtttgc atcgacaaac gccaattacg
       61 cgcaatgcag gtttcaatgc acaaacgtgt ggcatcatcc ttttggctaa tcggctgcag
      121 tgcaatgact cgcggtttat catcggtcag cgttgccagt agttcatcca gtgcttcaat
      181 atcacgtacg cgccccactg gatgcttgat ttcgttggct cgctccagtg cctgtgacaa
      241 cacttcatag ccgccgcgca tgttcagctt tggtgatacg gtaacccagg tattcggtgt
      301 gcagcgtacc tcatgagtac cactggtttc aatctggcag ctaaaaccat tcttttcgag
      361 cagatcagtc agtggtagca aatcatgaat gcaaggctca ccacccgtaa tcaccacatg
      421 ccgcgcggtg tatccctggc gaccaatgac agccagcaag tcttcactgc tcgccgcccc
      481 ccacttatca ctctctttgg tcttcgccag aatgctgaaa agggagactt cccgatcctc
      541 aagcttttcc caggtgtgtt tggtgtcgca ccaggcacag ccaaccgggc atccctgtaa
      601 acgaataaaa atggcgggga cgccggtaaa gtaaccctca ccttgcaggg tctggaacat
      661 ctcgttaatc gggtactgca tagcattctc tgtgaagtgg ataattgtta attattgcag
      721 atcctgccac aac
//
LOCUS       DAZKGF010000479          732 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZKGF010000479 DAZKGF010000000
VERSION     DAZKGF010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.482"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(95..316)
                     /locus_tag="ACHOQV_005350"
     CDS             complement(95..316)
                     /locus_tag="ACHOQV_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9633544.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            complement(379..>732)
                     /gene="radC"
                     /locus_tag="ACHOQV_005351"
     CDS             complement(379..>732)
                     /gene="radC"
                     /locus_tag="ACHOQV_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9633545.1"
                     /translation="AAREWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTE
                     VHPREVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLI
                     VGGNQVFSFAEHGLL"
BASE COUNT          166 a          188 c          212 g          166 t
ORIGIN      
        1 gcttcggtaa aggtgtattc gttgatgagc agggcctcat ccacagcggc gtctgagtcc
       61 atctggtcgt actcgttttc cgggtgacgg aacatcagtt cagggtgacg ctcatcagcc
      121 ggacatacgg gccaaaactg tccttatggc gttcagcaaa cacggccagc acaccgggaa
      181 tatcctgcac ttcacgaccg gtatacgctt cagtgctgcc gtgccagcgg tatttgccgg
      241 tacagaaagg aaaaagacgg gatgccggat gttgttggtg aatacgcatg gcttcaccac
      301 gggtgatgat tttcatagtg tgatacctct gaagacagaa gataaaagtg aaaacaagcg
      361 tgatgtggtt gtgacgggtt aaagcagacc atgttcggca aaggagaaaa cctggttgcc
      421 accgactatc agatggtccg gcacccggat atccaccagg cccagtgcct gtaccagacg
      481 ttccgtgata aggcggtctg ccttgctggg tgtgacttca ccggacgggt gattgtgcgc
      541 cagcaccacg gcagcggcat tgtggtacag ggcgcgttta atcacttccc ggggatggac
      601 ttccgtgcgg ttgatggtgc cggtgaagag ggtttcaccg gcaatcaact gattctggtt
      661 attcagatac agcacccgga attcttcacg ctccagtccc gccatgttca gaatcagcca
      721 ttcccgtgcc gc
//
LOCUS       DAZKGF010000480          718 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZKGF010000480 DAZKGF010000000
VERSION     DAZKGF010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.483"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          204 a          130 c          178 g          206 t
ORIGIN      
        1 attagagatg aaattaacaa agaacatttg acagctcatc aaaatagaat aaatgttgct
       61 gaggttgctg attctgacaa gagcgaattt agtagaatta aaacctcttc ggattttgat
      121 ggggcattat ggactgggat aagaaagctt atttcattct ttaaaaaata gatctatagc
      181 gtatcgttcc ggcgaactca tcctgatgcg ttttcggggg gccatcggtg aaacatacga
      241 atgatcgccc tctgacgatg gatgatttat tttgttgcac agaagcgatc gctgggcagg
      301 taccggaggt tggctctgaa gctctaattg cgtcaggttg cgaagtgttg acacgggtat
      361 tggtgagaac tgccccggga acgactctgg gctgattctg gttctgattc gctgtcagcc
      421 cgtgtggtta ctggcctggc tgatttcctg tcgtcaggcg tttaactccc gactgcaggt
      481 atcacttacc aagagaaggt aaatcctttt agacgtacag tgaaagttgt ctggtcataa
      541 tctgaagcat ctgacaaagt tgtgtccacc aaataataag tagtgggaac caaagtgtca
      601 tatatacaga aaaatataac tcaccgattt ccttcacgtt aatacgcagg gggaggtata
      661 taacagtccg ttcaggtatt ggataaatat ttagagtgga atgattacag ttatactg
//
LOCUS       DAZKGF010000481          714 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZKGF010000481 DAZKGF010000000
VERSION     DAZKGF010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.484"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>714
                     /locus_tag="ACHOQV_005352"
     CDS             <1..>714
                     /locus_tag="ACHOQV_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFP9633546.1"
                     /translation="EDVQKTGLITNTSPMSPFWRLILTIVIRPVQWLRDALVDVVLPG
                     MFVATADRSLLLLHAHDVNITPKPASKASGVIWFAKTRANDDVLIPEGTIVQTARING
                     VIYSMAVTEETLISSGKSGASVPVEATTEGSGHNLAPGYFRILPVAIPGIASVVNRED
                     WLTVPGADEESDDELRDRIRNQFNLVSSYHTDAVYRGLIASVAGLSIDRIYFQHDAPR
                     GPGTANAYLLLDSGEASAP"
BASE COUNT          156 a          224 c          198 g          136 t
ORIGIN      
        1 tgaggatgta cagaaaaccg gactcatcac caacacctcg cccatgagtc ccttctggcg
       61 gctcattctg accattgtca tacgacccgt tcagtggctg cgtgatgcgc tggttgacgt
      121 tgtgctgccc ggtatgtttg tcgccacggc tgaccggtca cttcttctgc ttcatgcgca
      181 cgacgtcaac atcaccccaa aaccggccag taaggccagt ggcgtgatct ggtttgccaa
      241 aaccagggca aatgatgatg ttctcatccc cgaagggacc atcgtccaga ccgccagaat
      301 taacggcgtg atatacagca tggccgtcac ggaggaaacg cttatcagca gcggtaaaag
      361 cggcgcatcc gttcccgtgg aagccaccac cgaaggcagc gggcacaacc tcgcgccggg
      421 gtacttccgt atccttcccg ttgccattcc cggcattgca tccgtggtga accgggaaga
      481 ctggctgacc gtaccgggtg ccgatgagga aagcgacgac gaactgcgcg acagaatacg
      541 caaccagttt aacctcgtga gcagttacca cacggacgcc gtctaccggg gacttatcgc
      601 cagcgtggcg ggcctgagca tcgaccggat ctactttcag cacgacgccc cgagaggacc
      661 gggaacggcc aacgcctacc tgttactgga cagcggcgag gcctctgccc cttt
//
LOCUS       DAZKGF010000482          704 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZKGF010000482 DAZKGF010000000
VERSION     DAZKGF010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.485"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>704)
                     /locus_tag="ACHOQV_005353"
     CDS             complement(<1..>704)
                     /locus_tag="ACHOQV_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9633547.1"
                     /translation="TTDQMEQAVFDYLLFGDLALLKVRNVFGDVVALEPLPSLYLRRR
                     KTGEFVVLQEGPALVYTPEDIIFLRMYDPRQQIYGLPDYIGGIHSALLNSEASLFRRR
                     YYNNGAHMGFLLYTNDPGITTEMEDEIREKVAESKGLGNFRNLYINIPKGSPDGVKII
                     PIGEVSAKDEFSNIKGISAQDVFTAHRFPAGLAGIIPTNGATIPNPETTRETYRKDEV
                     IPLQRKIMNAVNSDPD"
BASE COUNT          153 a          207 c          184 g          160 t
ORIGIN      
        1 atatcgggat cgctgttcac ggcgttcatg attttacgct gaagcggtat cacctcatcc
       61 ttgcggtacg tttcacgcgt cgtttccggg ttagggatgg tggccccgtt cgtggggatg
      121 atccccgcaa gaccggcagg aaagcggtgt gccgtaaaca catcctgggc gctgatcccc
      181 ttgatgtttg aaaattcatc cttggcgctc acctcaccaa tcggaataat tttcacccca
      241 tccggcgaac ctttggggat gttgatgtac agattgcgga aatttcccag ccccttagac
      301 tccgccacct tctcgcggat ctcatcctcc atctccgtgg tgatccccgg gtcgttcgta
      361 tacagcaaaa accccatatg cgccccgttg ttgtagtaac ggcgacgaaa aaggctggcc
      421 tcgctgttca gcagcgccga gtgaatgcca ccgatataat ccggcagccc gtaaatttgc
      481 tgacgcggat catacatccg cagaaagata atatcttccg gcgtatagac cagcgcgggg
      541 ccttcctgaa gcaccacgaa ctcaccggtt ttgcggcgcc ggagatacag cgacggaagc
      601 ggctcaagtg ccacgacgtc gccgaatacg ttacgcacct tgagcagggc gagatcgccg
      661 aacaacaggt agtcaaatac cgcctgttcc atctggtccg ttgt
//
LOCUS       DAZKGF010000483          702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZKGF010000483 DAZKGF010000000
VERSION     DAZKGF010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.486"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>702
                     /gene="espL1"
                     /locus_tag="ACHOQV_005354"
     CDS             <1..>702
                     /gene="espL1"
                     /locus_tag="ACHOQV_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFP9633548.1"
                     /translation="NDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFACQLITLLDTSD
                     LSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEIISLL
                     LETTPIKANHLRSILDYIEQEQLTTENRNHCMKLSKKIHREKTIQPTVNLNGSAFFSQ
                     SPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRSLLRHP
                     PAGSRVIASKDFGFA"
BASE COUNT          224 a          169 c          104 g          205 t
ORIGIN      
        1 aaaacgacat tattatcaga actcattata agtctcctca tagaatgcac atcgatagcg
       61 acataccaac gccttcatca gagcctatta atcaatttgc gtgccaactc atcaccctac
      121 ttgatacctc tgacttaagt tcaatgctgt catactgtgt tactcaggaa tttaccgcaa
      181 actgtcgaaa aatatcacaa aattgttatt ccactgccct ttttaccatt aactttgcca
      241 cttcacccat ccatgcagaa aatatactca ttacattaca ctataaaaaa gaaatcattt
      301 ccttattact ggaaaccacg cctattaaag ctaaccattt gcgaagcata ctggattata
      361 ttgaacagga acagttaact accgaaaatc gtaaccattg tatgaaactg tctaaaaaaa
      421 tccatagaga aaaaactata caaccaacag taaatctcaa tggtagtgca tttttttcgc
      481 aatctccttc tgacgctatt ttttgtcgcc atctgtcatt gcaatacgcc cttgattcac
      541 tgagaaatgg aaaaggcaaa gtcaatctga ttaaacatta ctcctccgtt gaatccatac
      601 agcagcatgt tcccttagtc cgggacgcgg agttcagatc attacttcgc catcctcctg
      661 cagggagtcg cgttatcgcg agtaaggatt ttggcttcgc tt
//
LOCUS       DAZKGF010000484          695 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZKGF010000484 DAZKGF010000000
VERSION     DAZKGF010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.487"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..652
                     /locus_tag="ACHOQV_005355"
                     /pseudo
     CDS             1..652
                     /locus_tag="ACHOQV_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
BASE COUNT          240 a          123 c          170 g          162 t
ORIGIN      
        1 gcggtgagtt atattgatgc tgccgtagcg ttgggtaata ccattaacga aatggataaa
       61 tattacatcc aggaaaatta taaagacgat gcgtttgcga aagggaaaac gctgcaccag
      121 acattcttaa aaaatctgga agcctttgaa cctgtagcag aatcttatca tgcggcgatt
      181 caggaaatta atgacaagcg ccagcttacg gaattgaaaa atattgaaga aagagaaggg
      241 aaaacattcc actactactc tctggcagtc atgatttcag cgaaacaaat taataacctg
      301 atatcgcaag ataagtttga tgcagaagcg gcaatgaaga aagtgtctga actggaaacg
      361 ctggtggcgc aggccaaaga agcggataaa agcggcatga atttctcgtt tattaattcg
      421 gcaggccagt atcaactcga ggctaaaaaa tacgttcgcc gcatcagaga taaagtcccg
      481 tactctgact gggataaaga gcaacttcag gacgcaaact caagctggat ggccgaagac
      541 tcatttcccg agagcattat gcgagtacaa cgaaatggtc gatgaaatat ttcagtcaat
      601 tgtaatcgcg gagcgggttt gtgatgagcg cggatatgtc acgaagtcgt gacaatataa
      661 agcagagtga aggataaatt ttgtgaataa agtat
//
LOCUS       DAZKGF010000485          689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZKGF010000485 DAZKGF010000000
VERSION     DAZKGF010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.488"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..627)
                     /locus_tag="ACHOQV_005356"
     CDS             complement(<1..627)
                     /locus_tag="ACHOQV_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFP9633549.1"
                     /translation="MNIRNNHLPGTETLFSETETANSKQLAVLKTNFPQCFDKNGAFI
                     QEKLLEIIRASNVEISKESYCLNWLGKSYARLLANLPPTTLLSEDKDHNRREENKNSQ
                     NLLIKGDNLEVLKHMVNAYSEKVKMIYIDPPYNTGSDGFAYNDDRKFTPEQLSDLAGI
                     DLDEAKRILEFTTNGSSSHSAWLTFIYPRLYIARELLREDGVIFISIDE"
BASE COUNT          199 a          136 c          141 g          213 t
ORIGIN      
        1 ttcgtcaatc gaaataaata tcacaccatc ttcacgtaga agctcacgag caatatataa
       61 acgaggataa atgaacgtta accatgcgct atggctgctt gaaccattcg tggtaaattc
      121 aagtatacgt ttagcctcat caagatcgat acctgccaga tcagaaagct gttctggtgt
      181 aaatttgcga tcatcattat aggcgaaccc atcagaaccc gtgttatatg gcggatcgat
      241 ataaatcatt ttaacctttt cagaataagc attaaccata tgctttaata cctcaaggtt
      301 atcccctttg attaacagat tttgactgtt cttgttctct tcacgtctgt tatggtcttt
      361 atcttctgac agcaatgtcg ttggtggtag gttagccagc aggcgagcat aggatttgcc
      421 aagccagttt aagcagtatg actctttaga gatttcaaca tttgatgctc tgatgatctc
      481 aagcagcttt tcctgaatga aagctccgtt tttatcgaaa cactgtggaa agttagtttt
      541 caaaacagca agttgtttac tgttcgcagt ttcagtttca gaaaaaagag tttctgttcc
      601 cggtagatga ttgttgcgta tattcataca gcacctccag aaagcacgga ggtagaggta
      661 cttgacggag agagttcagg cgagcatat
//
LOCUS       DAZKGF010000486          688 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZKGF010000486 DAZKGF010000000
VERSION     DAZKGF010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.489"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..675
                     /locus_tag="ACHOQV_005357"
     CDS             <1..675
                     /locus_tag="ACHOQV_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9633550.1"
                     /translation="MHFKSPYSGIVKLSDMEEGEIKQNVTLFDVLNYEYNSKILLIEK
                     KLQAECKKKRRLHNDYKESKHSYELGYISKDELLDIEDKINDSNMAIINLNIERNNIT
                     NILKLAKPLVSSPFIIRNIAVSNGQYVNSGDDIVDIELLDKFFIDVKIDPVSEQGNIK
                     EKKIRYHSLVNGLSGLASVVRITRTFENGQDDKSSGLRVVTLAIDGDHKKLAELLDTA
                     FEIEIK"
BASE COUNT          258 a           90 c          130 g          210 t
ORIGIN      
        1 atgcatttta agagcccata ctctggaatt gtgaaattat cggatatgga agaaggcgaa
       61 ataaaacaga acgtcacatt atttgatgtg ttgaattatg agtataattc caagattctt
      121 ttaattgaaa aaaaactaca ggctgaatgt aaaaaaaaac gtagacttca taatgactat
      181 aaagagagta agcattccta tgagttggga tatatatcca aagatgaatt acttgacata
      241 gaagataaaa taaatgattc gaacatggct attataaatc tgaatataga gagaaataat
      301 attactaata tattaaaact tgcaaaaccg ttggtatcat cgccttttat cataaggaat
      361 attgctgtaa gcaatgggca atatgtcaat tcaggagacg atatcgtaga tattgaactt
      421 ctggataagt ttttcattga tgttaaaatt gatcctgtgt ctgaacaagg aaatatcaaa
      481 gaaaaaaaga taagatatca ctctttggtc aatggattat ccggtttagc atctgtagtc
      541 cgcatcaccc gtaccttcga aaatggccag gatgataaaa gttctggttt gagggttgta
      601 acattggcca ttgatggtga tcataaaaaa ctcgctgaat tactcgatac tgcttttgag
      661 attgaaataa agtgattaaa ttagatat
//
LOCUS       DAZKGF010000487          684 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZKGF010000487 DAZKGF010000000
VERSION     DAZKGF010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.490"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>684
                     /locus_tag="ACHOQV_005358"
     CDS             <1..>684
                     /locus_tag="ACHOQV_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFP9633551.1"
                     /translation="LIESPEISGVDSGDKNIEEQSESELAPEQKRTGIAKMLMTTPVL
                     TLIFDRVRYDEIELQPDFQRKDRIWPDDKKSKLIESILMKLPLPGFYFGEKPNGNWVV
                     IDGLQRTTTICDYMSGHFSLKGLSILEHLNGKSFKDLTRTEQRDIREYQITAYQIELN
                     DDSSELVVELFHRINTYGVKLSSQEIRSALNKGNSVTFLRYLASLETFKKATQFKVKP
                     DRQKDMELC"
BASE COUNT          246 a          104 c          138 g          196 t
ORIGIN      
        1 tttaattgag tcccctgaga taagtggagt tgattcaggt gataagaata ttgaggaaca
       61 atcggaatca gaattagcac ctgagcaaaa acgtacgggc attgcaaaaa tgcttatgac
      121 tacaccagtt ttaacactaa tttttgatcg tgtaagatat gatgaaattg agttacaacc
      181 tgactttcag agaaaagacc gaatttggcc tgacgacaaa aagtcaaaac ttattgaatc
      241 aatattaatg aagcttccat taccggggtt ttactttggt gaaaagccaa acggtaactg
      301 ggtagttatt gatggccttc aaagaacgac tacaatttgt gattatatga gtggtcattt
      361 ctcattgaaa gggctctcta ttttagagca cttaaatgga aagagtttta aagatcttac
      421 tcgaaccgag caacgagaca ttagagagta tcaaattacg gcttaccaaa tagaactaaa
      481 tgatgatagt tctgaattgg ttgtagaact attccataga ataaacactt atggtgtaaa
      541 gttaagtagt caagaaataa gatctgcact taataaaggc aatagcgtaa cctttttacg
      601 ttatttagct tcattagaaa cgttcaaaaa agcgacgcaa tttaaagtaa agccggacag
      661 gcaaaaagat atggagctct gttt
//
LOCUS       DAZKGF010000488          679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZKGF010000488 DAZKGF010000000
VERSION     DAZKGF010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.491"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..222)
                     /locus_tag="ACHOQV_005359"
     CDS             complement(<1..222)
                     /locus_tag="ACHOQV_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFP9633552.1"
                     /translation="MARLLTIVLFFSISANAVTVNQFQRELDLADKTILWALISASTQ
                     EGRKACSLSYFSCKAAEAELGLAYMAANDN"
BASE COUNT          185 a          142 c          110 g          242 t
ORIGIN      
        1 gttatcatta gcagccatat atgccagtcc gagttcagcc tctgcggctt tacatgaaaa
       61 atagctaagt gaacacgctt ttcgtccctc ttgggtactg gctgatatta gtgcccataa
      121 tattgtcttg tctgccaaat ccaactctct ctgaaattgg tttacagtaa ctgcattcgc
      181 agaaatagag aaaaaaagca caattgttaa taaccttgcc atatttctag tccgtcactg
      241 ttaatgtgct atctggctct gaaaatatta gctcatttaa tttttgcatg tcattagatg
      301 aaccagttat acaaccctcg gaacagaatc gaggtcctaa agatggacca aaagcgttta
      361 aacaactatg tgttaaaaat ccatcacgat tatacgtttc tgtatttgct gacggtacta
      421 aacgtatacc attaggtttt ctattggttg ctccaggact gttaagcaag cctcgtccgg
      481 tttgcttcat tgagttctcc actttagact attggactat ctttcaacat ggcatcgcct
      541 tagtataccg ctggttttcc gctcattgtt aattttctct ttcgttcatt tcacttaatt
      601 ttcagttttt tccaaaagtc aatattccct tctaataaaa ttttttctat ggaatcttca
      661 ttaattttat tattagagc
//
LOCUS       DAZKGF010000489          672 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZKGF010000489 DAZKGF010000000
VERSION     DAZKGF010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.492"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(4..378)
                     /gene="tomB"
                     /locus_tag="ACHOQV_005360"
     CDS             complement(4..378)
                     /gene="tomB"
                     /locus_tag="ACHOQV_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFP9633553.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT          195 a          146 c          131 g          200 t
ORIGIN      
        1 attctaacaa gataaactcg caggattctc tttcgtcgca ttgacaaaac aacggaatag
       61 tcgattacct gacttccgcc atttctgaag atcctgcata ttaataccat aactactgaa
      121 caacataaag gtgtcatcca gatattcgtc gatctgctca atgagcttat tgtcttcatt
      181 atacttaatt ttgtaattaa gtgcgaaggt cgcaatatgc tcaatcagtt cattcaactg
      241 gaggttgatc gccgaggttg ggtcgttaac ccagccatga ttgctttctt caaggtttgc
      301 aagacagtca tgatacaggg tttcacagag aaacttaagc tgcgcgatat catgtctttt
      361 gggtgagtat tcatccataa cgcgtcccct tcttagcggt tgaactaacg gacacctttc
      421 gggatggaaa aaacttactg acctggactt gccctctcgt ttgttagctt aactataagc
      481 cactctttgc aggttttcat cgcattatta cgaaaaatta caattaggaa ggaagcggga
      541 caggactcac tttttcatac tagcactaac ccgcagcagg taaaagcagt ttgctcgaac
      601 tgttcataac atttcaggat tatcgccaga tgaaattggt aacactaata ccaggattgc
      661 tctgaatatg ac
//
LOCUS       DAZKGF010000490          671 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZKGF010000490 DAZKGF010000000
VERSION     DAZKGF010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.493"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..396
                     /locus_tag="ACHOQV_005361"
     CDS             <1..396
                     /locus_tag="ACHOQV_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633554.1"
                     /translation="KKIALIHKEEFAQDQHLFIKKTHYQVQLDYLASKGLNFSHSLAA
                     INDMIWSTSVQYGPTTGLISRALKGKNLETLTDADVITIVQDYKINHVEDLFPSSPTW
                     WDDLKTRALTEKKDLLELNELNLELKNED"
     gene            386..>671
                     /locus_tag="ACHOQV_005362"
     CDS             386..>671
                     /locus_tag="ACHOQV_005362"
                     /inference="COORDINATES: protein motif:HMM:NF045244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633555.1"
                     /translation="MKIKLFALLIFLPIISFAKEPHVCNSSMQSHPDIIDCIQISTRK
                     IEDSIKEIIAKSGNEYSIDMAFYNSQRKSISERCSLYYSMEGQRIDLIENQ"
BASE COUNT          239 a          110 c          119 g          203 t
ORIGIN      
        1 aaaaaaattg ctctcattca caaagaagaa ttcgcacaag atcagcatct ttttataaaa
       61 aaaacacact atcaagtaca attagactat ttggcatcca aagggctaaa tttctctcat
      121 tctcttgccg caatcaatga tatgatttgg tcgacttccg ttcaatatgg ccctacgact
      181 ggtttaattt cgagagcgtt gaaaggaaag aatttagaaa cattaacaga tgcagatgtg
      241 ataactattg ttcaggacta caaaataaat catgttgaag atctttttcc atcatccccg
      301 acttggtggg atgatttaaa gacacgagcg ctaacagaaa agaaagattt attggaatta
      361 aatgaattga atctggagtt aaagaatgaa gattaaatta tttgcattgc ttattttctt
      421 gccgattatc tcttttgcga aagaaccaca cgtttgcaat tcctcgatgc agtctcatcc
      481 agatattatc gattgcatac aaattagcac aaggaaaata gaggattcca ttaaagaaat
      541 aattgcgaaa tctggcaacg agtattcaat tgatatggcg ttttataata gccagagaaa
      601 gtctatttct gaacgatgca gcttatatta ttcgatggaa ggtcaacgaa ttgatttgat
      661 agaaaatcaa t
//
LOCUS       DAZKGF010000491          671 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZKGF010000491 DAZKGF010000000
VERSION     DAZKGF010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.494"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHOQV_005363"
     CDS             complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHOQV_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFP9633556.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT          157 a          187 c          125 g          202 t
ORIGIN      
        1 ccggaggttt ttcaaatgcg cctataaggc tctcttacca gccgcgccct aacaggcgca
       61 cacgatctga catttgcatc caacttcgtt acttacggcc cgtaaacggg ctgcccggat
      121 agggaatcga taactgctct cccattttat cctcttcaag ctggtgcttt atgtaatcct
      181 gtatcttcgc cgtgttctta cccaccgtat cgacgtagta ccctctgcac cagaactctc
      241 tgttcctgta tttgaatttc aaatcaccaa actgctcgta aagcatcaga ctgcttttcc
      301 ctttcagata tcccataaag ccggatacac tcattttggg cgggatctcc acaagcatat
      361 ggatatgatc tgcacagcat tcagcttcaa gaatccgtac gcttttccac tcacacagct
      421 ttctcaaaat actgcctatt gctctacgct tctctctgta gaacacttgt cttcggtatt
      481 ttggcgcaaa aactatgtga tatttacagt tccatcgggt gtgcgctaag ctcttttcgt
      541 tccccattgg gacccccttt tgatttcttg tttgacactt gcagttgcca gaccgcaagg
      601 tgttttaaca aatcaaaagg ggttttaata actggctcaa agctgaaagc tttccggaac
      661 ccccagccta g
//
LOCUS       DAZKGF010000492          670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZKGF010000492 DAZKGF010000000
VERSION     DAZKGF010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.495"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            446..>670
                     /locus_tag="ACHOQV_005364"
     CDS             446..>670
                     /locus_tag="ACHOQV_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633557.1"
                     /translation="MGHGTCKAAKSARHLIESKEKNRSSRVKVFSTHINLSHPAASWL
                     ATQTCANNIFNLLQNNNVIYLFENDNKIELR"
BASE COUNT          219 a          139 c          113 g          199 t
ORIGIN      
        1 cgccaaaatg cctcttcagt gatccccatc ctgcttattt ctgagcaacc tgaccctgct
       61 gatgtatctg gtagtatgac agagtgattc atttttcttg ccgagtgtac gaaaacgtct
      121 tccttgccac cgactatcaa ccgtcactca aaaaaacctc tctttttttc aagctatcaa
      181 gaataagttg cattttatct gcaccggaca ccagccatcc atatatggct tttacctgct
      241 acaaagatga agaagtgcat aaatgtgatg ttaaaacaat caaagaaaaa ttacatgtgc
      301 aataatttca ctataacatt ataatccatg gtgaaaataa cactgcacaa ggattgtcat
      361 catcaaactc atcggaacta ttttttctgt cgtcattggt gtaatactga ttgtaagtat
      421 cctttcagga gattattttt gccgtgtggg gcatggaact tgtaaagcag ccaaaagcgc
      481 taggcattta attgaaagta aagagaaaaa cagaagtagt cgcgtcaaag tctttagcac
      541 gcatattaat ttgagtcatc cagcggcatc ctggctagca actcaaactt gcgctaacaa
      601 tatattcaat ctacttcaaa acaataatgt catttattta ttcgaaaacg ataataaaat
      661 tgaactgcga
//
LOCUS       DAZKGF010000493          665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZKGF010000493 DAZKGF010000000
VERSION     DAZKGF010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.496"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>665
                     /locus_tag="ACHOQV_005365"
     CDS             <1..>665
                     /locus_tag="ACHOQV_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633558.1"
                     /translation="GIHITEATTPWCALSGKSPLEAIDFPVPFKGEQAIRCMADGEVI
                     AYRICRDYLTIAWESGPLNFSGSFVLVKHYIQPGEKETSGLHFYTLYMHLAPYSAYES
                     EAENQWVVQDTLKAYSEMDWLTAKLTSDETSPQIAGYMPEGARVEWNPADSNLNATGS
                     NKRKYGLVTLKGLPEKNSSTLTPGQRYWVVVDNNNVKPAPGAGPGWWRHLLPPAKEAM
                     VFD"
BASE COUNT          180 a          153 c          177 g          155 t
ORIGIN      
        1 gggatccata tcaccgaagc cacgacacca tggtgtgcgc ttagcggtaa gtctccactt
       61 gaagcgatag attttccggt tccttttaaa ggtgagcagg caatccgatg catggctgac
      121 ggagaagtga ttgcttaccg catctgcagg gattatctga cgattgcatg ggaatccggt
      181 ccgctgaatt tttccggctc atttgttctg gtgaaacact atatccagcc gggtgagaaa
      241 gaaaccagtg gactgcattt ctatactctg tatatgcatc ttgcgccgta ttcagcttat
      301 gaatctgaag cagaaaatca gtgggtcgtt caggacactc taaaagcata ttctgaaatg
      361 gactggctga cggcgaaact gacttcagat gaaacttctc cacaaattgc agggtacatg
      421 cctgaagggg ccagagtgga gtggaacccg gcagacagca atcttaacgc tacaggaagt
      481 aacaagagaa aatacggtct tgtgacgcta aagggtttac cagaaaaaaa cagcagtact
      541 ctcactcccg gtcagcgcta ctgggttgtt gtggataaca acaatgttaa gccagcgccg
      601 ggggccgggc ccggctggtg gcgacacttg ctgcctcccg caaaggaagc catggtattc
      661 gataa
//
LOCUS       DAZKGF010000494          664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZKGF010000494 DAZKGF010000000
VERSION     DAZKGF010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.497"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..>664
                     /locus_tag="ACHOQV_005366"
     CDS             44..>664
                     /locus_tag="ACHOQV_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFP9633559.1"
                     /translation="MAKYSDEIKEAARSLYLKRWHPRDIAAELGIPPRTVYHWCDVGQ
                     WATLLPAESVEDVIARRIDVLTGREGKTECELAELRELIAQHVKIIAQKNKHAERMAE
                     IAALRPAGHEDGVAAGTCGGGEGKKRRYRKNDVSGLTAEMLDEWARKNLYAYQLHCRE
                     NRHRERRFILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAE"
BASE COUNT          147 a          170 c          219 g          128 t
ORIGIN      
        1 tttctgcctg aatgcgggcc ggatgcgccg ccataatggg cgcatggcta aatattctga
       61 cgaaatcaaa gaggcggcgc gatcgcttta cctgaagcgg tggcaccccc gcgatattgc
      121 ggcggaactc ggtattccgc cgcgcacggt ttaccactgg tgtgatgtgg ggcagtgggc
      181 taccctgctg cccgctgaat ccgttgagga cgtgatcgcc cggcgtattg atgtgctgac
      241 cggacgggag ggcaagacag agtgtgaact tgccgaactg cgtgaactta tcgcgcagca
      301 cgtgaagatc atcgcccaga aaaacaaaca cgctgaaagg atggcggaaa ttgccgccct
      361 gcgtccggca gggcatgagg acggcgtggc tgcggggacc tgcggcgggg gagaagggaa
      421 aaagcggcgc taccggaaaa acgacgtttc cggactgact gctgaaatgc tggatgaatg
      481 ggcgcgtaaa aatctttatg cctaccagct gcactgccgc gagaaccggc accgtgagcg
      541 ccgtttcatc ctcaagagcc gccagatcgg catgacctgg tattttgcat gggaggcgtt
      601 cgaagatgcg gtggtgaccg gtgataacca gattttcttt tctgcgtcac gggtacaggc
      661 ggaa
//
LOCUS       DAZKGF010000495          659 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZKGF010000495 DAZKGF010000000
VERSION     DAZKGF010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.498"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            55..>659
                     /locus_tag="ACHOQV_005367"
     CDS             55..>659
                     /locus_tag="ACHOQV_005367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9633560.1"
                     /translation="MGDILDRLQARFGRNIEGNVAAQQALDDAFGSGADVIKKLWGQQ
                     EMLNRSISALGRNDGMKRATEMAEAMTDPWDRLVASFYNIRAAIGNALYPILAPLAGR
                     FADIGATFVRWLNMFPNIARWLGYITLTMLALGAAGAVTNIVLGVFSFIMEGHKAIIK
                     GARAAWSLYTLALTKVRSAIIATSLAARLAGVSFLTMSAPVL"
BASE COUNT          132 a          189 c          205 g          133 t
ORIGIN      
        1 gccgcacaaa aaatggggat gagttttgtc gatgtcaccg ggaggatttt acccatgggt
       61 gacatcctcg accgtctgca ggcgaggttc ggcaggaaca ttgagggtaa cgtggcggca
      121 caacaggcgc tggatgacgc cttcggcagt ggcgcggacg tgattaaaaa actctggggg
      181 cagcaggaaa tgcttaaccg gagcatatca gcgctggggc gtaatgacgg catgaagcgt
      241 gccacggaaa tggcggaagc catgaccgac ccgtgggatc gccttgtggc ctccttctac
      301 aacatccggg ccgccatcgg taatgccctc taccccattc tcgcaccact ggctggccgt
      361 tttgccgata tcggcgcgac gttcgtgcgc tggctgaaca tgttcccgaa catcgcccgc
      421 tggctgggtt acatcacgct gaccatgctg gcgctgggcg cagccggtgc ggtcacgaac
      481 atcgtgctgg gtgtgttcag cttcatcatg gaaggccata aggccatcat aaagggcgcc
      541 agggcagcct ggagtctgta cacgcttgca ctcacaaaag tacgcagtgc catcattgcc
      601 accagtcttg ccgcgcgtct ggcgggcgtg tcgtttctga ccatgagcgc cccggtcct
//
LOCUS       DAZKGF010000496          656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZKGF010000496 DAZKGF010000000
VERSION     DAZKGF010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.499"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..419
                     /locus_tag="ACHOQV_005368"
     CDS             <1..419
                     /locus_tag="ACHOQV_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633561.1"
                     /translation="LKYLSQAPRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEV
                     DAIRNNGHANGKWAAAAISQNGHMWDAMPGSYYKMGSDWDGGEFDDHLTLELEKNGKG
                     SRIYITYASPAPGHLKDKFIEPLVKRVKDVAEGRIR"
BASE COUNT          213 a          113 c          181 g          149 t
ORIGIN      
        1 atctcaaata tctgtctcag gcaccccggg caaaaagaga gaaacgtgat catgtcatag
       61 aagtccctgt tgatgtggat acagctgcgg gaagactgaa acggtactat aagttttatt
      121 caaatgaaga ggtcgatgcg attcgtaata acggacatgc gaacgggaag tgggccgcag
      181 cagcaataag tcagaacgga catatgtggg atgccatgcc cggtagttat tataaaatgg
      241 gcagtgactg ggatggtggc gaatttgatg atcatctgac gcttgaactt gaaaaaaatg
      301 gcaaaggaag tcggatatat ataacgtatg catctccggc accggggcat ctgaaagata
      361 aatttattga gccattagtt aaacgtgtga aggatgtggc tgaaggccgg atcagataaa
      421 attaacccgc tggtattgtc gttgtgatgg gctcctctgg aggggcgatt atgctcagag
      481 gggctgaaag taagggacgc agagggcgac gaccagcgac ctctggaaga gggcgtgata
      541 tgtaaggaag cacaccctga cggcatatca taaaaaaacc agatatgcgc cagttaaaag
      601 tataaataaa gagctgataa aattaacata ttttgaaaat gatgaattta tacgcc
//
LOCUS       DAZKGF010000497          654 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZKGF010000497 DAZKGF010000000
VERSION     DAZKGF010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.500"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            144..>654
                     /locus_tag="ACHOQV_005369"
     CDS             144..>654
                     /locus_tag="ACHOQV_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633562.1"
                     /translation="MSIDFTPCMINTYHGDIYNRTTDTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYTSDQYDLAQYQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAEYRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFI"
BASE COUNT          253 a          126 c          104 g          171 t
ORIGIN      
        1 tggaaatgaa agggaagtta aaggtaatta tatcaggaag atatgacgca ttgtgtttat
       61 ttccgcgtag aagaaaatcc attcctgctc taattctccg atttacaaaa atttagcgaa
      121 atagatgaac aataaggaat gttatgtcaa ttgactttac cccatgtatg ataaatacat
      181 atcacggcga tatttataac cgcacaacag ataccgacaa tgttaaaaca ccagacacac
      241 cgaagtggcc ttgcgataat tgggaagaac aacaacccat taattccaca ttctctggag
      301 aaggatatac ctctgatcaa tatgatttag cgcaatacca acttcaacaa attaatgcgt
      361 gccacacgaa cactacatat acaaatgcag actactcaaa agttgtggct caacttgtaa
      421 gtcttattac taacattgaa acgataagtt cgacacaact tacgcaacag acacaatcaa
      481 tacttaatca gattaataac attagatacg aaaaaaacaa aagtgctgaa tatcgaatca
      541 ttgttgtcgc taatcctaaa cccgacaagg caatcataac taaaattagc gtagaagagg
      601 gaataccagt aagattcagt gtccaaacca tgttttcaga tactaatttt atta
//
LOCUS       DAZKGF010000498          650 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZKGF010000498 DAZKGF010000000
VERSION     DAZKGF010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.501"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..399
                     /locus_tag="ACHOQV_005370"
     CDS             <1..399
                     /locus_tag="ACHOQV_005370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633563.1"
                     /translation="IDDCLNELYRSTVVVKNLTQHDHIWYLTGYSRKDASKHVVFSDN
                     EPNQNTIDTFERHYKKNRPLINESIWNGQPDGLACSISHFMKFIDNPKKLNLIIDIDQ
                     RVANTSDMIDSLLLLAECGMDVIYAPHTLT"
     gene            377..580
                     /locus_tag="ACHOQV_005371"
     CDS             377..580
                     /locus_tag="ACHOQV_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm52 family immunity protein"
                     /protein_id="HFP9633564.1"
                     /translation="MPHILLPELIPEAAKIVPVFEGEKQKGTIVVSTEDVFDGNNKEH
                     IGKANDIEIRLLDLGLLPLLTEL"
BASE COUNT          217 a          119 c          126 g          188 t
ORIGIN      
        1 atagacgatt gtttgaatga actatacaga agtaccgtag ttgttaaaaa tctaacgcag
       61 cacgatcaca tatggtactt aactggctac tcaagaaagg acgcttcaaa gcatgtcgtg
      121 tttagtgata atgaaccgaa ccaaaataca atagatacct ttgagcggca ttataaaaag
      181 aatcgtcctt taattaatga atctatatgg aatggacaac ccgatgggct ggcttgctca
      241 atatcacatt ttatgaaatt tattgataac cctaaaaaac taaatcttat catagatatt
      301 gatcagagag ttgccaatac ttcggatatg attgattcct tgcttctgtt ggctgaatgt
      361 gggatggatg ttatatatgc cccacatact cttacctgaa ttaatccctg aagccgcaaa
      421 aatagtaccg gttttcgagg gcgagaagca aaagggaact attgttgttt caaccgaaga
      481 cgtatttgac ggaaataata aggaacacat cggaaaagct aatgatattg aaataagact
      541 tctggatctt ggactgctac ctttgctaac agagctttaa tccaacctta tcactcatgg
      601 gtagatgacg ggacagttac acccgttaat ggccttattt ttaacacatc
//
LOCUS       DAZKGF010000499          637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZKGF010000499 DAZKGF010000000
VERSION     DAZKGF010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.502"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..423
                     /locus_tag="ACHOQV_005372"
     CDS             <1..423
                     /locus_tag="ACHOQV_005372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633565.1"
                     /translation="LKPRQGTDDLAVALLIIYRRYPVVTKKKDKINRDVVISLRLTEK
                     EFGDFVDDIKKSQLSRSAFFRKLILEKSSSVVINVNNTAPLVYHFNKAGNNLNQLAYK
                     VNSAFLMGRISESLYVKVLNCLVDIRELLMTGVNNNAR"
     gene            413..>637
                     /locus_tag="ACHOQV_005373"
     CDS             413..>637
                     /locus_tag="ACHOQV_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFP9633566.1"
                     /translation="MLVRFSGHNNGAKEYLEEGKKNGRELTRDELDTRITLYGDLELT
                     QKIYQSIPDNDQERYVSITHSYFEDDISVDV"
BASE COUNT          206 a           85 c          132 g          214 t
ORIGIN      
        1 ttgaaacccc gtcaggggac ggacgacctg gcggtggcat tgttaattat ttacaggagg
       61 tatcctgtgg tcactaaaaa aaaggacaaa attaacaggg atgtcgttat atccttaaga
      121 ttgacagaga aagaatttgg cgattttgtt gatgatataa aaaaatctca actcagtcgt
      181 tctgcatttt tcagaaaact gattcttgaa aaatcatcat ctgtcgtaat taatgttaat
      241 aatacggctc ctttagtata tcactttaat aaagctggta ataatcttaa ccagcttgct
      301 tataaggtta actcggcttt tttgatgggg cgtatttctg aatccttata tgtaaaagta
      361 ttaaattgtc ttgttgatat tagggagtta ttaatgacag gagttaataa taatgctcgt
      421 taggttttct ggtcataata atggagcaaa agaatatctt gaagagggta aaaaaaatgg
      481 gcgagaattg actcgtgatg agctggatac cagaattact ttatatggtg atttggaatt
      541 aacccaaaaa atttatcagt ctatacctga taatgatcag gagcgttatg tttcaattac
      601 acattcgtat tttgaggatg atatttctgt tgatgtg
//
LOCUS       DAZKGF010000500          635 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZKGF010000500 DAZKGF010000000
VERSION     DAZKGF010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.503"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(113..598)
                     /gene="sixA"
                     /locus_tag="ACHOQV_005374"
     CDS             complement(113..598)
                     /gene="sixA"
                     /locus_tag="ACHOQV_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFP9633567.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT          164 a          172 c          150 g          149 t
ORIGIN      
        1 ccattcgatc acaagcggat cattgttagt acaataagat tgcataaatt caacattgcc
       61 agaaaaaaat aaaggcccga tagattacac cagcgggcct tgtgcatgcg tattaaatcg
      121 cttttgccat cttcagatta cacgggctca tctgccagtt gaatgtgccg tttccgcttt
      181 catcaagcgt tacgctggca atggccgagg tggtaaacat aggcggcgtt tcgcccggac
      241 ataactcggc aaccagatac ccgactaagg gtaagtggga gatcaccagt accgaagcaa
      301 caccttcatt ggtcaacgcc tgcaaatagg cactgaccag accaacatcg ccgcagggcg
      361 ttaactccgg cagaacttct gcactggaag gcaggttcag acaatcccct acttcttcaa
      421 gtgtttgctc ggctcgcagg aacgggctca ccagaacgcg ttcgatttcc actttttgac
      481 ctttcagcca gttcgccatc aggcgagatt cgtcacaacc gttagtggtc agaggacgaa
      541 ccgaatcact ggcggcatcg agggctgcgt cgccgtgacg catgataaaa acttgcatat
      601 tgcaccgctt ttgttaacca gattcgcccg ttttc
//
LOCUS       DAZKGF010000501          631 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZKGF010000501 DAZKGF010000000
VERSION     DAZKGF010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.504"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(481..631)
                     /locus_tag="ACHOQV_005375"
                     /pseudo
     CDS             complement(481..631)
                     /locus_tag="ACHOQV_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          188 a          139 c           96 g          208 t
ORIGIN      
        1 agatatcgta aaagaccagt gcaattctat gtaaactctc tggctgttca ttttatttgt
       61 tgtttcaggg tcggaactca ccctcaataa acaactgaat acgagtagat attcatcgtc
      121 tgagctatat ggctttacac aatagccgac atgtcttttc tcagtcaggg attatacttt
      181 tcctcactac gaagttgacg acttcgatat gggatagact cttaattcaa gcaattactc
      241 tgcacaagca tcaggtaacc cagctccaat atttgttata aaataatgcg taacattaat
      301 ttcaccaaca aaaaaaaggt ttacgctatc cccttgccct ggtattttac catatttaca
      361 ccacactttt tttgtatctg tatcattctc tgatacgatt ttcgccactt gttccctggc
      421 cctcacagtc aatgttttct taaacctccc cccatcatcg attgttaggg aaaaataaga
      481 aaaaaaataa tcatcttcta catttgtgga tattgatata tccaccgcta cgtgcggaaa
      541 aaaatctggg agccatgtaa aaaccccatc actttctggt attgccatat agtttatgta
      601 atgcttactt acaagagatt catctttgtg t
//
LOCUS       DAZKGF010000502          628 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZKGF010000502 DAZKGF010000000
VERSION     DAZKGF010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.505"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..445)
                     /locus_tag="ACHOQV_005376"
     CDS             complement(<1..445)
                     /locus_tag="ACHOQV_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFP9633568.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSVASMMAQMQMGNVKALFESKPFITLSEPACGAGSMILAM"
BASE COUNT          168 a          175 c          139 g          146 t
ORIGIN      
        1 ccattgccag aatcatactg ccggcaccac aggcaggttc actgagcgtg ataaagggtt
       61 tactctcaaa caacgccttc acattcccca tctgcatctg tgccatcatg ctggccacac
      121 tccatggcgt gaaaaactga ctacggtatt tatcccccag ttccagttgc atgaacacac
      181 tgcccagaaa atcacagaat ccctgctcca gtccctcaat gacatgagca agcaaatgcg
      241 caatgtggat aacatcctgc ttttcataac cactgacgat ccgcagatac ttctgttccc
      301 gggcctcact gaactgaagc cggttttcca gggcaatcac actacagctg acaaagtctt
      361 caaacacctt atgacgtttg tgataacagg ctgtccggtt aaacaacgaa atgaactcct
      421 tctgatggtc agtcatggtg gccatgtttt ctccgtaaaa acagggaaca catggcccgg
      481 acaggggaac aggtgttccc cttcgggttg aaatgttgat aaaacgggca tcacggacat
      541 gatacccgga ggttaattac tgggctgaga ccagaggaac cggtaaaggt gcggctttcg
      601 tctgttcttc agcgcactcc tccaccag
//
LOCUS       DAZKGF010000503          628 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZKGF010000503 DAZKGF010000000
VERSION     DAZKGF010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.506"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..445)
                     /locus_tag="ACHOQV_005377"
     CDS             complement(<1..445)
                     /locus_tag="ACHOQV_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFP9633569.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSVASMMAQMQLGNVKALFESKPFITLSEPACGAGSMILAM"
BASE COUNT          170 a          170 c          139 g          149 t
ORIGIN      
        1 ccattgccag aatcatactg ccggcaccac aggcaggttc actgagcgtg ataaagggtt
       61 tactctcaaa caacgccttc acatttccca gttgcatctg tgccatcatg ctggcaacac
      121 tccagggcgt gaaaaactga ctccggtatt tatcccccag ttccagttgc atgaacacac
      181 tacccagaaa atcacagaat ccctgctcca gtccctcaat gacatgagca agcaaatgcg
      241 caatgtggat aacatcctgc ttttcataac cactgacgat tcgcagatac ttctgttccc
      301 gggcctcact gaactgaagc cggttttcca gggcaatcac actacagctg acaaagtctt
      361 caaacacctt atgacgtttg tgataacagg ctgtccggtt aaacaacgaa atgaattcct
      421 tctgatggtc agtcatggtg gccatgtttt ctccgtaaaa acagggaaca catggcccgg
      481 acaggggaac aggtgttccc cttcgggttg aaatgttgat aaaacgggca tcacggacat
      541 aatacccgga ggttaattac tgggctgaga ccagaggaac cggtaaaggt gcggctttcg
      601 tctgttcttc agtgcactcc tccaccag
//
LOCUS       DAZKGF010000504          626 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZKGF010000504 DAZKGF010000000
VERSION     DAZKGF010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.507"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..587)
                     /gene="fimE"
                     /locus_tag="ACHOQV_005378"
     CDS             complement(<1..587)
                     /gene="fimE"
                     /locus_tag="ACHOQV_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFP9633570.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKR"
BASE COUNT          174 a          158 c          130 g          164 t
ORIGIN      
        1 tctcttttta atttttcgtt tatgagatta tttctttccc ataatccggc aaaacgagca
       61 gcattactgg cggtataacg tacagtatgg cgaatatttc gatgcccgag ataatcctga
      121 attaaacggg tatcggcacc acgctccgcc agttcataac cgcaagcatg ccttaacata
      181 tgaggatgag tctgcgttac ggttccagct tcaatacctg catcgcgaat aatgcgatag
      241 gcctgctggc gagaaagccg actcccgcgg cgagaaataa atatagcgtc ggtccggtca
      301 gcgcctttcc agttagcacg ttcctgggtc cagcgttcca cggcttcacg ctcatcaaaa
      361 cgtaacgggt gaacggtaga aaatccgttc ttcagtcggc gaatatttat tcgaccttca
      421 ttaaggtcaa gatcctgata atgcagatca agcagttcac taatacgcat cccatgccga
      481 tatgccaaca gaataagaca ataatctctg gctcccgttg ccccgtaaca aaccgcctgc
      541 atcatggcct gaacttcttt accggtaaga taacgacgtt tactcacaat agtagtactc
      601 ctaactgaga tatatttgaa tgaata
//
LOCUS       DAZKGF010000505          611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZKGF010000505 DAZKGF010000000
VERSION     DAZKGF010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.508"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            10..>611
                     /locus_tag="ACHOQV_005379"
     CDS             10..>611
                     /locus_tag="ACHOQV_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP9633571.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDE"
BASE COUNT          162 a          147 c          160 g          142 t
ORIGIN      
        1 gagttcgaaa tggaccagaa gctgttaacg gatttccgct cagaactact cgattcacgt
       61 tttggtgcaa aggccatttc tactatcgcg gagtcaaaac gatttccgct gcacgaaatg
      121 cgcgatgatg ttgcatttca gattatcaat gatgaattat atcttgatgg caacgctcgt
      181 cagaacctgg ccactttctg ccagacctgg gacgacgaaa acgtccataa attgatggat
      241 ttgtcgatca ataaaaactg gatcgacaaa gaagaatatc cgcaatccgc tgcaatcgac
      301 ctgcgttgcg taaacatggt tgccgatctg tggcatgcgc ctgcaccgaa aaatggtcag
      361 gccgttggca ccaacaccat tggttcttcc gaggcctgta tgctcggcgg gatggcgatg
      421 aaatggcgtt ggcgcaagcg tatggaagcc gcaggcaaac caacggataa accaaacctg
      481 gtgtgcggtc cggtacaaat ctgctggcat aaattcgccc gctactggga tgtggagttg
      541 cgtgagatcc ctatgcgccc cggtcagttg tttatggacc cgaaacgcat gattgaagcc
      601 tgcgacgaaa a
//
LOCUS       DAZKGF010000506          601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZKGF010000506 DAZKGF010000000
VERSION     DAZKGF010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.509"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>601)
                     /locus_tag="ACHOQV_005380"
     CDS             complement(<1..>601)
                     /locus_tag="ACHOQV_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9633572.1"
                     /translation="IYGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLV
                     QRKTDLAITLQPVISRSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLIS
                     KSAGIDEIQMDIDERFINRKVSFRSSSLLTIINSISVTDLLGIVPTELYDLHRDFLKL
                     KEIKLEQPLPAVKLYISYNKASLNNLVFSRFIDRLNDSF"
BASE COUNT          193 a           92 c          131 g          185 t
ORIGIN      
        1 cagaagctat catttaaacg atctatgaat cgggagaaga caaggttgtt caacgaagcc
       61 ttgttatagg aaatataaag tttaaccgca ggaagaggtt gttctagttt tatttctttt
      121 aacttcaaaa aatcacgatg taaatcgtac agttcggtgg gcacaatgcc taataagtca
      181 gtaacagaaa tgctattaat tatagtcaac aaagaagaac tgcgaaatga aactttacgg
      241 tttataaaac gttcatctat gtccatttga atttcgtcaa tccctgctga tttagatatc
      301 aacagggtga attcttcggc gataatttgt tcatagttac tggtatcagt aatacgagga
      361 tgtttgttgc tacatataag ggaattgcga atagtgtgta atggcatgca gatgacagag
      421 cgactaatta ctggttgcag tgtaattgcc aggtcagttt tgcgttgaac caacagttct
      481 tcggcatttt cagctgaaag caggatgtca tggcattcta tatcgattga agtatcttgt
      541 ctaagacagc ggattagcat gttgttatta gtacaagaaa taagctgagg accgtagata
      601 a
//
LOCUS       DAZKGF010000507          597 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZKGF010000507 DAZKGF010000000
VERSION     DAZKGF010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.510"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>597
                     /locus_tag="ACHOQV_005381"
     CDS             <1..>597
                     /locus_tag="ACHOQV_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633573.1"
                     /translation="ARTEGDATALAAARAHSDENDKRTLVSANTYADQKETAINNRTD
                     GLLTTERQARTAGDTATLAAARTHSDENNKRTLISANTYADQKETAINNRTDGLLATE
                     RQARTAGDAATLAAARTHSDENNKRTLISANTYADQKETAINNRTDGLLMTERQARIA
                     GDAETLATAEAYSDAGDSLTLKQATAYSDISTRRALSEA"
BASE COUNT          197 a          173 c          148 g           79 t
ORIGIN      
        1 gcccgaacag aaggtgatgc aacagcgctg gctgcagcca gagcccacag cgatgaaaac
       61 gacaaaagaa cactggtcag tgcgaatacc tatgctgacc agaaagaaac cgccatcaat
      121 aaccgtactg acggattact gacgaccgaa cgccaggcac gcaccgcggg cgatactgca
      181 actctggctg ctgccagaac gcacagcgat gaaaacaaca aaagaacact gatcagtgcg
      241 aatacctatg ctgaccaaaa agaaaccgcc atcaataacc gtactgacgg attactggcg
      301 accgaacgcc aggcacgcac cgcaggcgat gctgcaactc tggctgctgc cagaacgcac
      361 agcgatgaaa acaacaaaag aacactgatc agtgcgaata cctatgctga ccagaaagaa
      421 accgccatca ataaccgtac tgacggatta ctgatgaccg aacgccaggc acgcatcgca
      481 ggtgatgcag aaactctggc aacagcagag gcatacagtg acgccgggga ttcactgaca
      541 ctgaaacagg caacagccta cagcgatatc agtacccgcc gggcactgtc agaggcc
//
LOCUS       DAZKGF010000508          597 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZKGF010000508 DAZKGF010000000
VERSION     DAZKGF010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.511"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>597
                     /locus_tag="ACHOQV_005382"
     CDS             <1..>597
                     /locus_tag="ACHOQV_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633574.1"
                     /translation="ARTEGDAAALAAARAHSDENDKRTLVSANTYADQKETAINNRTD
                     GLLTTERQARTAGDTETLAAARTHSDENDKRTLVSANTYADQKETAINNRTDGLLMTE
                     RQARIAGDTATLAAARTHSDENNKRTLVSANTYADQKETDINNRTDGLLMTERQARIA
                     GDAETLATAAAYSDAGDSLTLKQATAYSDISTRRALSEA"
BASE COUNT          194 a          169 c          152 g           82 t
ORIGIN      
        1 gcccgaacag aaggtgatgc agcagcgctg gctgcagcca gagcccacag cgatgaaaac
       61 gacaaaagaa cactggtcag tgcgaatacc tatgctgacc agaaagaaac cgccatcaat
      121 aaccgtactg acggattact gacgaccgaa cgccaggcac gcaccgcggg cgatactgaa
      181 actctggctg ctgccagaac gcacagcgat gaaaacgaca aaagaacact ggtcagtgcg
      241 aatacttatg ctgaccagaa agaaaccgcc atcaataacc gtactgacgg attactgatg
      301 accgaacgcc aggcacgcat cgcgggcgat actgcaactc tggctgctgc cagaacgcac
      361 agcgatgaaa acaacaaaag aacactggtc agtgcgaata cctatgctga ccagaaagaa
      421 accgacatca ataaccgtac tgacggatta ctgatgaccg aacgccaggc acgcatcgca
      481 ggtgatgcag aaactctggc aacagcagcg gcatacagtg acgccgggga ttcactgaca
      541 ctgaaacagg caacagccta cagcgatatc agtacccgcc gggcactgtc agaggcc
//
LOCUS       DAZKGF010000509          596 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZKGF010000509 DAZKGF010000000
VERSION     DAZKGF010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.512"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            156..>596
                     /gene="alaE"
                     /locus_tag="ACHOQV_005383"
     CDS             156..>596
                     /gene="alaE"
                     /locus_tag="ACHOQV_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFP9633575.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADIMAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQV"
BASE COUNT          145 a          126 c          140 g          185 t
ORIGIN      
        1 atattttatc tacaaaaaca gattaaataa aaatttttca ctaattgatt agtcatagcc
       61 agcgatatac gctatgcaaa aatgcagatg gcaatgagat ccactgcttt catctccatt
      121 cacatcccat tacgctttta ttaaggagca ttagcatgtt ctcaccgcag tcacgcttgc
      181 gtcatgcagt tgcagatacg ttcgcgatgg ttgtttactg ttctgtcgtg aacatgtgta
      241 ttgaagtttt cctctccgga atgagcttcg aacagtcttt ttattccaga ttggtagcga
      301 ttccggtaaa catcttaatt gcatggccat acggtatgta ccgcgatctg tttatgcgcg
      361 cagcacgcaa agttagcccg tcgggctgga taaaaaatct ggcggatatc atggcttatg
      421 tgacgttcca gtcaccggtg tatgtggcga tcttgttagt ggtgggcgca gactggcatc
      481 agattatggc ggcggtcagt tcaaacatcg ttgtttcgat gttgatgggg gcggtttatg
      541 gctacttcct cgattattgc cgccgactgt ttaaagtcag ccgttaccag caggta
//
LOCUS       DAZKGF010000510          595 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZKGF010000510 DAZKGF010000000
VERSION     DAZKGF010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.513"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>595
                     /locus_tag="ACHOQV_005384"
     CDS             <1..>595
                     /locus_tag="ACHOQV_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP9633576.1"
                     /translation="AGQRWTTEQFVEWLNERGAFRPGLWIFKANISLSRHSVLTDTGY
                     GDICLGGCLIEVSGDINGGIIRITTGLESPNPADMYLRTQFTGVVLNKGKSTIWYRTY
                     TDKNKPTPQDIGALPASELLPAGVPLPWPSDTPPAGYAIMVGQTFDKARYPLLAKAYP
                     DGRIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHGG"
BASE COUNT          157 a          170 c          158 g          110 t
ORIGIN      
        1 aagcagggca acgctggaca acagaacaat tcgtggaatg gctgaatgag cgtggcgcct
       61 tcagaccagg actctggata ttcaaagcca atatttcact ttcccggcac agcgtgctca
      121 ctgatacggg ctatggtgat atctgccttg gaggctgcct tatcgaagta tcaggcgaca
      181 tcaacggggg gatcatcagg atcacaacag gtctcgaaag cccaaaccct gccgacatgt
      241 acctccgcac acagttcacc ggggttgttt taaataaagg gaaaagcacc atctggtacc
      301 gcacttacac ggacaaaaac aaacccacac cacaggacat cggcgcactg cctgcaagtg
      361 agttgttacc cgcaggcgtt cccctgccgt ggccgtcaga caccccgcct gccggttacg
      421 ccatcatggt ggggcagaca ttcgataaag cacgatatcc cctgctggca aaagcctatc
      481 cggatggtcg tatcccggat atgcgtggct ggacaatcaa gggaaaacct gccagcggtc
      541 gtgcggtatt gtcacaggaa caggacggca tcaaatccca cacccacggg gggag
//
LOCUS       DAZKGF010000511          595 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZKGF010000511 DAZKGF010000000
VERSION     DAZKGF010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.514"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>595
                     /locus_tag="ACHOQV_005385"
     CDS             <1..>595
                     /locus_tag="ACHOQV_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP9633577.1"
                     /translation="AGQRWTTEQFVEWLNERGAFRPGLWIFKANISLSQHSVLTDTGY
                     GDICLGGCLIEVSGDINGGLIRITTGIESPEDITRPHRTQFTGVILSKGKSAIWYRTY
                     TDKNKPTPQDIGALPASELLPAGVPLPWPSDTPPAGYAIMVGQTFDKAQYPLLAKAYP
                     DGRIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHGG"
BASE COUNT          161 a          170 c          156 g          108 t
ORIGIN      
        1 aagcagggca acgctggaca acggaacaat tcgtggaatg gctgaatgaa cgtggcgcct
       61 tcagaccagg actctggata ttcaaagcca atatttcact gtcacagcac agcgtgctca
      121 ctgatacggg ctatggcgat atctgccttg gaggctgcct tatcgaagta tcaggcgaca
      181 tcaacggggg gctcatcagg atcactacag gcattgaatc ccctgaagac atcaccagac
      241 ctcaccgcac acagttcacc ggagttattt taagtaaagg gaaaagcgcc atctggtacc
      301 gcacttacac ggacaaaaac aaacccacac cacaggacat cggcgcacta cctgcaagtg
      361 agttgttacc cgcaggcgtt cccctgccgt ggccgtcaga caccccacct gccggttacg
      421 ccatcatggt ggggcagaca ttcgataaag cgcaatatcc cctgctggca aaagcctatc
      481 cggacggtcg tatcccggat atgcgtggct ggacaatcaa gggaaaacct gccagcggtc
      541 gtgcggtatt gtcacaggaa caggacggca tcaaatcgca cacccacggg gggag
//
LOCUS       DAZKGF010000512          590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZKGF010000512 DAZKGF010000000
VERSION     DAZKGF010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.515"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>590
                     /locus_tag="ACHOQV_005386"
     CDS             <1..>590
                     /locus_tag="ACHOQV_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9633578.1"
                     /translation="TLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVL
                     DVLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLE
                     RARQCGAGDDDYLFDVCRFSEPISADRYQYCPVRAHQTVRSFFRRLSRECGFPVSPHR
                     FRHTLATRLMKYPERNLHLVKSILGHRSLTTTMGYV"
BASE COUNT          143 a          129 c          162 g          156 t
ORIGIN      
        1 acactgagtg acaatcagat gctcagggtt tacctggttg tgcagaggtt tgcagaagcg
       61 gaagagcaga acatggtgaa tagtcgtcga cgcaatgcta tataccctgc ctggtactgg
      121 ctgacagttc tggatgttct gcgttatacc ggaatgcgct ttaatcaact gaagcatatc
      181 cgtgtgaggg atgtctgtcc ggatgaaagc ataattgatc ttaagctgga gggaagtaaa
      241 actcacaggg aatggggcgt tcctctggtg gctcccctga aggtatcgat ggaaaaactg
      301 ctggagcggg cccgtcagtg cggggcaggt gatgatgact atctttttga cgtctgtcgt
      361 ttcagtgagc cgatctctgc cgacagatat cagtactgtc ccgttcgggc gcatcagacc
      421 gtacgttcat ttttccggcg actttcgcga gaatgtggtt ttcctgtttc accacatcgt
      481 ttcagacata cgctggcaac cagactgatg aaatatcctg aacgtaattt acatctggtc
      541 aaatccatac tgggacacag gagtcttaca acgaccatgg ggtatgttga
//
LOCUS       DAZKGF010000513          588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZKGF010000513 DAZKGF010000000
VERSION     DAZKGF010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.516"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            39..413
                     /locus_tag="ACHOQV_005387"
     CDS             39..413
                     /locus_tag="ACHOQV_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9633579.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRNQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPAQAPATTS"
     gene            460..>588
                     /locus_tag="ACHOQV_005388"
     CDS             460..>588
                     /locus_tag="ACHOQV_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9633580.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPF"
BASE COUNT          138 a          187 c          134 g          129 t
ORIGIN      
        1 taaaccattc ttaaccactt ttcagagagg attttatcgt gtcagacaaa ctctccggga
       61 taactcatcc cgacgacaat cacgaccgcc cctggtgggg gcttccctgc acagtgaggc
      121 cctgttttgg tgcccgtctg gtgcaggagg gtaaccgcct gcattacctt gccgaccgcg
      181 ccggtatcag aggccgcttc agcgacgcag atgcatacca tctggaccag gcctttccgc
      241 tgctgatgaa acaactggag ctcatgctca ccagcggtga actgaatccc cgaaatcagc
      301 ataccgtcac gctgtatgca aaagggctga cctgcgaagc cgacaccctc ggctcctgtg
      361 gttacgttta tctggctgtt tatccggcac aggcacccgc aaccacctca taaccccgaa
      421 gctctcacct ttagcactac cagttacaga gaacttaaga tgaaaacatt atccgacaca
      481 catgtacggg aggtatctcg ctgcccgtct cccgtcacca tctggcagac actgctcatc
      541 cgactgctgg accagcatta tggcctcaca ctgaatgaca caccgttt
//
LOCUS       DAZKGF010000514          573 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZKGF010000514 DAZKGF010000000
VERSION     DAZKGF010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.517"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..195
                     /locus_tag="ACHOQV_005389"
     CDS             <1..195
                     /locus_tag="ACHOQV_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633581.1"
                     /translation="QTRIKQGLIAKAPLEGWHYGPKEIVKKFHMYHCVAIEYSGEIYD
                     IDNLRRAHLICMMKFITGDS"
     gene            196..486
                     /locus_tag="ACHOQV_005390"
     CDS             196..486
                     /locus_tag="ACHOQV_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9633582.1"
                     /translation="MYNFKDKVEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDD
                     LEEHLTRITQHPLMSDLIYYPAKKGDDKPENILKIVKEWRRSQGLSLFKDSE"
BASE COUNT          202 a           92 c          124 g          155 t
ORIGIN      
        1 caaacacgta taaaacaagg attaatcgca aaagcacctc ttgaaggttg gcattatggc
       61 cctaaagaaa tcgttaaaaa attccacatg tatcattgtg ttgcgattga atatagtggc
      121 gaaatttacg atattgataa tttaaggagg gcacacctca tttgcatgat gaaatttatt
      181 acaggagata gttaaatgta taattttaaa gataaagtag aagattacac agagcgggaa
      241 ttcattgagt tgcttggcga gtttacaaat ccaaccgggg ataacgctca attaaaaggg
      301 gaagagcttg acaaatattg ggatgatcta gaagaacatc ttaccagaat tacacaacat
      361 ccgttgatga gcgatcttat ctactatcct gcaaagaagg gagatgataa gcctgaaaac
      421 atcctgaaaa tcgtcaaaga atggcggcgt tcgcaaggat tgtctttgtt caaagattca
      481 gaatgaattc taaacagaat ctgtcacctg aaaatcatca cgttctggtt aagtgatgat
      541 tttcgctacg agaaggttac ggtggcgatt gca
//
LOCUS       DAZKGF010000515          572 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZKGF010000515 DAZKGF010000000
VERSION     DAZKGF010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.518"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(76..438)
                     /locus_tag="ACHOQV_005391"
     CDS             complement(76..438)
                     /locus_tag="ACHOQV_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633583.1"
                     /translation="MKKYILVSLMGLSLNAFAGGSGWNPDRVDPSRCVMATGSGPTSA
                     GYMVYRTTDSVCMQGINEGKVKGVHIYDGHHEFNDGTVDTFSGNVTPISPLTLKTDVQ
                     KVNKVGIKRWWYKASWIK"
BASE COUNT          166 a          136 c           89 g          181 t
ORIGIN      
        1 gaaaaaaata caccgaatgc aaagattcgc attcgatgtg tttaagttat ttattaacgt
       61 ttggcaaggt attttttatt taatccagct agctttatac caccagcgtt tgatgccaac
      121 tttattaact ttttgaacat cagtttttaa tgtcaaaggt gatattgggg taacattacc
      181 gctaaacgtg tcaacagtac catcattgaa ctcatgatgc ccatcataaa tatggacacc
      241 tttcactttt ccttcattta taccctgcat acacactgag tcagtcgttc ggtataccat
      301 ataacccgca gaggtaggac ctgaccccgt cgccataaca caacggctcg ggtctaccct
      361 gtctgggttc cagccgctgc cacccgcaaa tgcgttcaaa cttaatccca tcagagatac
      421 taaaatatat ttcttcatta taacctctta ttgttaatag ataatatatt tgtcatgcaa
      481 caagttgcat gtactgcact tacgctccct cagtcactta cttatgcaag ctcttgggaa
      541 ctcacttata cactacctgt aactcgaatt at
//
LOCUS       DAZKGF010000516          568 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZKGF010000516 DAZKGF010000000
VERSION     DAZKGF010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.519"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>568)
                     /locus_tag="ACHOQV_005392"
     CDS             complement(<1..>568)
                     /locus_tag="ACHOQV_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP9633584.1"
                     /translation="RSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQAL
                     PEELRLSPHRYLATNSPQGPWWVLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQ
                     TFHREAGGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLP
                     GYTEYGRDNGIRLSAVWLTHDPEYPDNLP"
BASE COUNT           99 a          205 c          171 g           93 t
ORIGIN      
        1 ggcaggttgt ccggatattc cgggtcgtgc gtcagccaca cggcagacag acggatgccg
       61 ttgtcccggc catattcggt gtaacccggc agggtatcag gaaaagcgga cggttccgtc
      121 ccgccggaaa tggcctgctg ccgggcctct tctgcccgct gcgcctgcgt ggtcagtacc
      181 agccggaagt gacgcccggc accatccgtc acgccggtga tttcgccgct gaattcgccg
      241 ccagcctcac ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc
      301 cggtacggcg gcagcggcgc aggcagcacc tcatccgcct ccggcacccg ctcacaccag
      361 ccgagtaccc accacggccc ctgcggactg ttcgtcgcca gataacgatg cggactcagg
      421 cgaagctctt ccggcagcgc ctgccagagt gcggccagcc gatgaccttc atccagtttc
      481 gccacgccgc cgcgcaccag ccagagtgac tcgctgcggc tgtaaccgtc ctcaccggga
      541 aacaggtgct caaaatacag gcttctgc
//
LOCUS       DAZKGF010000517          567 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZKGF010000517 DAZKGF010000000
VERSION     DAZKGF010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.520"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          156 a          159 c          129 g          123 t
ORIGIN      
        1 tttaccttct ccgggtgagt gatacctgcc gtcaggtgtt cgtgcaccgg gccataacct
       61 gcagtcggga gttgaacgcc tgacgacagg aaatgagcca gaccagcaac ctcacgggct
      121 gacagcgaat aagaacctga atcagcccgg agtcattccc gggtcagtac atcatcacct
      181 gccattaatc cccgagtcag tacttcgcgg cctgacgcga tcaccctgtg caacaaaata
      241 aatcaaccgt tatcagacca cgatcgttcg tatgcgccgc cgatgacgcg cgcctgaaaa
      301 acgcgtcaag atgagatgcc cggaagcact ctgctgactg ggatcaaacg ccagaagagc
      361 agaggtaagc aaaaaagtcc gccaactaag gacgggatta aatttatgct ttagggactg
      421 atggcggaga ttttctggct ttacgttcat ctatccatgc aaagatccat gatcttaacc
      481 cagccccccc ccctgcgatc ccactcaatg ttatggctgc tttaaattgt ttttctgaaa
      541 aatagaaaca acctccttcc caaaaat
//
LOCUS       DAZKGF010000518          565 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZKGF010000518 DAZKGF010000000
VERSION     DAZKGF010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.521"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>565
                     /locus_tag="ACHOQV_005393"
     CDS             <1..>565
                     /locus_tag="ACHOQV_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFP9633585.1"
                     /translation="EQEALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVSR
                     RIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPC
                     CPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGI
                     AGPGLLARVLTSKYAEHTPLYRQSEIYG"
BASE COUNT          127 a          172 c          170 g           96 t
ORIGIN      
        1 ccgaacagga agcgctgatc cacgccctgc aggaaaagct gagcaaccgg gagcgcgaaa
       61 tagaccatct gcaggcgcag ctggataaac tccgccggat gaacttcggc agtcgttccg
      121 aaaaagtctc ccgccgtatc gcacaaatgg aagccgatct gaaccggctt cagaaagaga
      181 gcgatacgct gactggtagg gtgtatgacc cggcagtaca gcgtccgttg cgtcagaccc
      241 gcacccgtaa gccgttccct gaatcactac cccgtgacga aaagcgactg ttgcctgcgg
      301 cgccgtgctg cccgaactgc ggcggttcac tgagctatct gggcgaggat accgccgaac
      361 agctggagtt gatgcgtagc gccttccggg ttatccggac ggtacgggaa aaacatgcct
      421 gtactcagtg cgatgccatc gtgcaggcac ctgcaccttc gcggcccatc gagcggggta
      481 tcgccggacc ggggctgctg gcccgcgtgc tgacctcgaa gtatgcagag cacaccccgc
      541 tgtatcgcca gtcagaaata tacgg
//
LOCUS       DAZKGF010000519          563 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZKGF010000519 DAZKGF010000000
VERSION     DAZKGF010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.522"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            315..512
                     /locus_tag="ACHOQV_005394"
     CDS             315..512
                     /locus_tag="ACHOQV_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633586.1"
                     /translation="MILSRKKIEIEVVQSGTADNPETVTKIKVSRPEDLEACGVIVEK
                     ICAIHAKSVNTTASNDHNKYN"
BASE COUNT          159 a          124 c          133 g          147 t
ORIGIN      
        1 catggttaag cataaaatgc tcacgatgat cgttttttgc ggcatagcgc ttgactcctt
       61 ttacagggag gcgctaacct tccacttgtc acggtaggaa gtgttagggc ctcgggttaa
      121 catttaagtt gactcggggc ctttccacat ccggccttca ggtaatcctt ccagccatca
      181 gccgaaaggc acccgcgtgt aatgtactga tttccctttg tgtgcgcaat gcctggcgtt
      241 gaagcagaca acgctatcgc actggtgata ttctttcgca caggcttact ctgtcctgtg
      301 tcggggcgat gaaaatgatt ctttcaagaa aaaaaattga aattgaggtt gtgcaaagtg
      361 gaacggctga taatcctgag acggttacaa aaataaaggt ttcacgcccg gaagatcttg
      421 aagcgtgtgg tgttatcgtg gaaaaaattt gtgctattca tgcaaaatca gttaatacca
      481 ctgccagtaa cgaccacaat aaatacaact gaacaaaaaa gcccgcatat tgcgggcaaa
      541 tacgtctgta atccaacgga gaa
//
LOCUS       DAZKGF010000520          563 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZKGF010000520 DAZKGF010000000
VERSION     DAZKGF010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.523"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>563
                     /locus_tag="ACHOQV_005395"
     rRNA            <1..>563
                     /locus_tag="ACHOQV_005395"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          155 a          119 c          172 g          117 t
ORIGIN      
        1 gggacacgtg gtatcctgtc tgaatatggg gggaccatcc tccaaggcta aatactcctg
       61 actgaccgat agtgaaccag taccgtgagg gaaaggcgaa aagaaccccg gcgaggggag
      121 tgaaaaagaa cctgaaaccg tgtacgtaca agcagtggga gcctctttat ggggtgactg
      181 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      241 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      301 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      361 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      421 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      481 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
      541 accggagaat gttatcacgg gag
//
LOCUS       DAZKGF010000521          561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZKGF010000521 DAZKGF010000000
VERSION     DAZKGF010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.524"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..201)
                     /locus_tag="ACHOQV_005396"
     CDS             complement(<1..201)
                     /locus_tag="ACHOQV_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFP9633587.1"
                     /translation="MKTSDNESTKYAITGQAVLQILRMKINFSLQTLIKQLLVMKSVE
                     ENAFRRDLIDSIIRDFSNSDTGG"
     gene            complement(244..480)
                     /gene="ycgZ"
                     /locus_tag="ACHOQV_005397"
     CDS             complement(244..480)
                     /gene="ycgZ"
                     /locus_tag="ACHOQV_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFP9633588.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRIL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          166 a          120 c           98 g          177 t
ORIGIN      
        1 ccctcctgta tcactgttgc taaaatcacg aattatgctg tcaattaaat ctcgtcggaa
       61 agcattttcc tcaacagatt tcattacgag taattgctta ataagagtct gcaacgaaaa
      121 atttatcttc atgcgcagta tctggagcac cgcctggcca gtaattgcat attttgtact
      181 ttcattatca gatgtcttca taacgacttc atagctaacc tgttaaataa tcaggctgtt
      241 gcattattca aaaagcaacc caattagtgc attataatgt ttctgttcct cttcccctgt
      301 cgcttgttcc agtcggcata gaagtttggc acaaagcgac tttcgactca gaatacgtcc
      361 atctttcaaa atctcagtca caatttcccc gagagtttcc tgctgagtag gcaagttagc
      421 ttttgcaaag taacgagtga ttgctcccgc agaatctaaa gtcactgaat tttgatgcat
      481 gctacgcctc tgttaaaaat gttaatcaat gttaagtgag gtaacaatag caaacttgta
      541 cgaaatatgt gtacagaatg a
//
LOCUS       DAZKGF010000522          554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZKGF010000522 DAZKGF010000000
VERSION     DAZKGF010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.525"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..548
                     /locus_tag="ACHOQV_005398"
     CDS             <1..548
                     /locus_tag="ACHOQV_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HFP9633589.1"
                     /translation="VFNHIEAWMSEISPGHRISPRIEARANVAFNVIIPSPGQLTKPI
                     NIGFGFSYALSIVTALMLASKNELVIIENPEAHLHPRGQSYLGRLIALSAKAGIQVII
                     ETHSDHLLNGIRVIARTYPTFDASLFTLYYISRENDETSVAKIKINNEGKLSEWPRGF
                     FDQQSQDMYTIMTGSFDHPNI"
BASE COUNT          176 a          100 c           98 g          180 t
ORIGIN      
        1 atgttttcaa tcatattgaa gcatggatgt ctgagatttc accagggcat cgtatctcac
       61 ctagaattga ggctagggcg aatgttgcat ttaatgtcat aatcccttca cctggacaat
      121 taacgaaacc tattaacata ggatttggat ttagttatgc tcttagtatt gtcactgctt
      181 taatgctggc atcaaaaaat gaactcgtta ttattgaaaa tcctgaggct catctacatc
      241 caagaggtca aagctattta ggacgactta ttgctttatc agcaaaagca gggatccagg
      301 ttattattga gactcatagt gatcatcttc ttaatggcat aagagtaatt gcacgcacat
      361 atccaacttt tgatgcttct ctttttactc tttactatat ttcacgagaa aatgatgaaa
      421 catctgtcgc caaaataaaa ataaataatg aagggaaact ttccgaatgg cctagagggt
      481 tctttgatca acaatcacag gatatgtata cgataatgac aggctctttt gatcatccca
      541 atatttaata ttgt
//
LOCUS       DAZKGF010000523          552 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZKGF010000523 DAZKGF010000000
VERSION     DAZKGF010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.526"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            312..>552
                     /gene="gadE"
                     /locus_tag="ACHOQV_005399"
     CDS             312..>552
                     /gene="gadE"
                     /locus_tag="ACHOQV_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP9633590.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNNPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKL"
BASE COUNT          194 a           84 c          103 g          171 t
ORIGIN      
        1 attttaactt tagtaatatt cttcagagat cacaaactgg ttattgataa cttattcttg
       61 ggcagtaatc cgcaaaagtt aaccttttgt ttactagtta caaactgata acaaccagga
      121 atcttactta ggaccaatat atggagtgcg tgatggataa atctgaagta ttgattagtg
      181 ttaatagacg tattagttca cgaagggcaa agttcttata ggcgtttact atattgaaca
      241 acgattcgga caaggatgta aataatgaaa aggatgacat gttcgaaacg ataacggcta
      301 aggagcaagt tatgattttt ctcatgacga aagattcttt tcttttacag ggcttttggc
      361 agttgaaaga taatcacgaa atgataaaaa tcaattccct gtcagagatc aaaaaagtag
      421 gcaataaccc cttcaaggtt atcattgata cctatcacaa tcatatcctt gatgaagaag
      481 cgattaaatt tctggagaaa ttagatgccg agagaattat tgttttggca ccttatcaca
      541 tcagtaaact aa
//
LOCUS       DAZKGF010000524          552 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZKGF010000524 DAZKGF010000000
VERSION     DAZKGF010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.527"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..386
                     /locus_tag="ACHOQV_005400"
     CDS             <1..386
                     /locus_tag="ACHOQV_005400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633591.1"
                     /translation="LSIYHVICSVTSKINIKNSAKFNIRSTSRLTYDIISQLHLPQNG
                     YFMERSNFLVAIIMLILRSLAIIPAWFVSLGHEIVTFPFILEIWGNAGDAFSSTSVGN
                     YGFGHYDLNLPQSQLFAGRYGKQTK"
BASE COUNT          171 a          127 c           94 g          160 t
ORIGIN      
        1 tcttatctat atatcatgtg atatgttctg taacaagtaa aatcaacata aaaaattcag
       61 caaaatttaa tattcgttca actagccgtc tcacatatga cattattagt caactgcatt
      121 tgccacaaaa tggctatttc atggaacgca gtaatttctt agtggctatt atcatgctaa
      181 ttcttagatc cctagcgatt attcctgcct ggttcgtcag cttaggccat gagattgtta
      241 cattcccttt tatcctggag atatggggga acgcaggcga cgctttttca tctacatccg
      301 ttggtaacta tggctttggt cactacgact taaaccttcc tcaatcacag ctattcgccg
      361 ggcgatacgg caaacaaact aagtgaaaca tcatcctgaa aacaccaaca tcaacaagcc
      421 tctccagatc gacatcagga gtgaccagtt acaagccaca aaccaagaaa catatttcat
      481 gatgagaatt atgctcagcc tgatggcggg taaacactta ttttcaccaa gctcactgag
      541 ttagcttgca aa
//
LOCUS       DAZKGF010000525          544 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZKGF010000525 DAZKGF010000000
VERSION     DAZKGF010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.528"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..468
                     /locus_tag="ACHOQV_005401"
     CDS             <1..468
                     /locus_tag="ACHOQV_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP9633592.1"
                     /translation="KGGWNFYQYPLNPVSGFDPLGLKVSFQGDESTQKTLKEAYKAVA
                     ETKFGHKITEELESSEHEYIFRGLRKGINQTCYDDTEYSFYIDIDNDHSSCVYQGKNK
                     ACAMKPTLLSVVLAHEMGHAKGMKDDGTDSMANVDKYENPFRKELGLPARMKY"
BASE COUNT          197 a           70 c          120 g          157 t
ORIGIN      
        1 aaggggggat ggaattttta tcagtatccg ttgaatccgg tatctggttt cgatccatta
       61 gggttaaaag tgtcttttca gggagatgaa agtacacaaa aaactttaaa agaagcatat
      121 aaagctgtag cagaaacaaa gtttggacat aaaataacag aagaattaga gtcatcagag
      181 catgaatata tatttagagg attaagaaaa ggcataaacc aaacatgcta cgatgatact
      241 gaatatagtt tttacattga tattgataac gaccatagct catgtgtata tcaaggaaaa
      301 aacaaagcat gtgccatgaa acccactctg ttgagtgtgg ttcttgcgca tgaaatgggg
      361 catgcaaagg gaatgaaaga cgatggaact gactcgatgg ctaatgttga taaatatgaa
      421 aacccatttc gtaaagagtt gggcttgcca gcaagaatga aatattaaac atttttatat
      481 tgcttttggg tatgttttta atatatcacc agtttaggag atggtatgaa agtatgtctg
      541 tatt
//
LOCUS       DAZKGF010000526          537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZKGF010000526 DAZKGF010000000
VERSION     DAZKGF010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.529"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          161 a          131 c          122 g          123 t
ORIGIN      
        1 ttaaacgttg cgatccttcg ggtagtgact gcctgtgcgc cagggtagcg cagattgttt
       61 aggtattgag gggtaacaaa tcggtgttgc acaccgactg cagcatttgc tgtacctgtc
      121 tccaggtcgc ccccgacacc acctctaata agtggtatcg ccgattcggc atctcactac
      181 ataaaactcc ttaaaaaaca attgattctg caaaaaacca tttatggacg ttattcacaa
      241 aatcgtccgt aaatttgcag aagaggttga aaaaacactc tcacgccggg tcacttcttc
      301 aaatgttaaa aaacatcacg tgaaacggca gttcgtgcgg gttcgttaag cagacgcgcg
      361 tgcgtcactt tgtcatcgtg acacgctgac gaaagtgacg ccgcgcagct tcagacgcca
      421 gatacacgcg taagcgacca aatttcaaat ggtcttatac agcgcgcaca gtcgcaggaa
      481 gtgctggcaa acaaatgaaa agaataaaag aagtcaatac cgccattacg cagtaaa
//
LOCUS       DAZKGF010000527          536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZKGF010000527 DAZKGF010000000
VERSION     DAZKGF010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.530"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..472)
                     /gene="reiD"
                     /locus_tag="ACHOQV_005402"
     CDS             complement(<1..472)
                     /gene="reiD"
                     /locus_tag="ACHOQV_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator ReiD"
                     /protein_id="HFP9633593.1"
                     /translation="MAYVSQVSESNGPFVRFMHMHPRTVELILITEGKGEYFIGDRIY
                     PVRKGDLVIYNSEVVHDEYLESGRPVGTICCGINNIVCPGLRENALIPDDTVPVIPLY
                     HHYATIEKLMSSIFNVINQHTVEGPTMAQLLTRVLLKYVEGNVLMRTGNDITRRH"
BASE COUNT          145 a          140 c          100 g          151 t
ORIGIN      
        1 tatggcgccg tgttatatca ttacctgtgc gcattaatac atttccctcg acgtatttaa
       61 gcagcaccct ggtcagcaac tgcgccattg ttggtccttc tactgtatgc tgatttataa
      121 cattaaatat tgaactcatc aatttctcga tcgtcgcata atgatgatat agtggaatta
      181 cgggcacagt atcatctgga atcagagcat tctcccgcag acccgggcac acaatattgt
      241 ttatcccaca gcaaatagta cccacaggcc gtccgctttc aagatactca tcatgcacta
      301 cttcgctgtt ataaataacc aaatcacctt ttctgactgg gtaaatgcga tcaccgatga
      361 aatactcccc ctttccctcg gtgataagga ttagctccac ggtcctggga tgcatatgca
      421 tgaaccgtac aaacgggcca ttgctttcgc taacctgact gacatacgcc aactgagccc
      481 gggacttatt atcttgttct gaaggacaga agcaaaaacg catgattttt cctcca
//
LOCUS       DAZKGF010000528          534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZKGF010000528 DAZKGF010000000
VERSION     DAZKGF010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.531"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            133..303
                     /locus_tag="ACHOQV_005403"
     CDS             133..303
                     /locus_tag="ACHOQV_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633594.1"
                     /translation="MNSTRRISDRELTKMIESAQQLLSRQNPVVEETWWENLLAALTE
                     LRERRNNEGAAK"
     gene            300..>534
                     /locus_tag="ACHOQV_005404"
     CDS             300..>534
                     /locus_tag="ACHOQV_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9633595.1"
                     /translation="MKTPGMTGKEIILRYLETHARFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEKERQELAQRA"
BASE COUNT          179 a          133 c          149 g           73 t
ORIGIN      
        1 accagaacac tggcatcgat taaccgcaaa gcggcaattg cccgctatgt gcattcagcc
       61 gctggcagac aatcacgcag gtaaacaaag accatgaaca taaccagaaa cagccaacta
      121 aaggagcgca ccatgaacag caccaggagg atcagcgaca gagagcttac gaaaatgatt
      181 gagtcggcgc agcagctgct ttcacgacaa aacccggtcg tggaagaaac atggtgggag
      241 aatttactgg cagcactgac cgaactccgg gaacgccgga acaacgaagg agccgccaaa
      301 tgaagacccc aggaatgacc ggaaaggaaa tcattttacg ctacctggaa acgcacgcac
      361 gtttcagcgc cccagaactg gtagaaaaac tgggcgtgaa aagaaccgtc attaatcagg
      421 ccgccggaaa aatggtacgg gaagggttgc tggttgtgga cgggtacacg gacaagataa
      481 cgcattaccg gaagccaacg gaaaaggaac gacaggaact ggcgcaacgg gctg
//
LOCUS       DAZKGF010000529          534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZKGF010000529 DAZKGF010000000
VERSION     DAZKGF010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.532"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>534
                     /locus_tag="ACHOQV_005405"
     CDS             <1..>534
                     /locus_tag="ACHOQV_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9633596.1"
                     /translation="LTLKNFKAYKEQSFDFSNLTVFCGNNSVGKSTAIQAIGMFLQSD
                     MNGNSNLKINGDLVHVGDFDDIHNFSMREDDVLYLKYTFEDDTACWGYGIEEGDAREE
                     LSRKNELPFIGCDKQLRTITNSLKDNFSFQYLEAERFGPRDNIPLSQHNYHEGWLGKN
                     GEHTIEVLESLVTRRRL"
BASE COUNT          188 a           64 c          111 g          171 t
ORIGIN      
        1 tttaacatta aaaaatttca aagcttataa agaacagagt tttgatttta gtaatttaac
       61 tgtattttgc ggtaataatt ctgttggtaa aagtacggct attcaagcta tcggtatgtt
      121 tctccaatca gatatgaatg gtaacagtaa tctaaaaata aatggcgatt tagttcatgt
      181 cggagacttc gacgatattc ataatttcag tatgcgtgag gatgatgttt tatatttaaa
      241 atatacattt gaagacgata cggcttgctg gggatatggt atagaagaag gggatgctag
      301 agaagaactt tctaggaaaa atgaattacc ttttattggg tgtgataaac aattaagaac
      361 tataacaaac tcacttaaag ataacttttc attccaatat ttggaagcgg agagatttgg
      421 tccaagagat aatattccat tatcacagca caattatcat gaaggttggc ttgggaaaaa
      481 tggggaacat acaattgaag ttcttgagag tttggtcaca aggaggcgtt taaa
//
LOCUS       DAZKGF010000530          533 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZKGF010000530 DAZKGF010000000
VERSION     DAZKGF010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.533"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>533
                     /locus_tag="ACHOQV_005406"
     CDS             <1..>533
                     /locus_tag="ACHOQV_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFP9633597.1"
                     /translation="YCFIAIALLAGVFSFKDTLLTRMNDLNRDLVNYSHDNTRTSVGA
                     RLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDLIDTLSTR
                     GIPGVALTILAFSAIFIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTI
                     ILLCAYFKAQSDQCLLD"
BASE COUNT          164 a           90 c           95 g          184 t
ORIGIN      
        1 atattgtttt attgcgattg cattattagc aggtgtattt tcttttaaag atacattact
       61 tacgagaatg aatgacttaa atagggattt agttaattat tcgcatgata acaccagaac
      121 ttcagtcggt gcccgtctgg caatgtatga agttggttta aaaacatatt ctccaatagg
      181 acaatcactg gaaaaacgtg cggaaaaaat acatgaacta gaagagaaag agcctagatt
      241 aagtggcgct ttaccctttg tagattctca tttgcataac gatctcatag atacgttatc
      301 aacgcgtggt attcctggag tagcattaac aattttagcc ttttcagcaa tattcatata
      361 tgccttaaga actgctaaag aaccttatat tttaatcttg cttttttcac tactggtagt
      421 aggcctaagt gatgtaatac tcttttctaa accggttccg actgctgtgt ttgtcaccat
      481 aatattgctt tgtgcttatt ttaaagcaca atcggaccaa tgtttattag ata
//
LOCUS       DAZKGF010000531          531 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZKGF010000531 DAZKGF010000000
VERSION     DAZKGF010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.534"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>531)
                     /locus_tag="ACHOQV_005407"
     CDS             complement(<1..>531)
                     /locus_tag="ACHOQV_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP9633598.1"
                     /translation="SCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEA
                     CWSWLSDYPEQAQPCDMLMVIPSRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGN
                     NVRHARDEMSSLVLLIDTPWYPPSGELVGEISKVFDCEVRHWYSEPVHGIQGYNCYDG
                     GEHTDSDLQAEWPGRET"
BASE COUNT          119 a          172 c          127 g          113 t
ORIGIN      
        1 gtttccctgc ccggccattc ggcctgcaga tcactgtccg tatgctctcc gccatcgtaa
       61 cagttgtaac cctgtatacc gtgtaccggc tcgctgtacc agtgtcggac ctcgcagtca
      121 aacaccttcg agatctcacc aaccagttcg cctgaaggcg gataccaggg cgtgtcgatc
      181 agcagcacca gactgctcat ctcatcacgc gcatggcgaa cattatttcc tgacggccac
      241 tccacaccgt atatccggct gtaaaggctg gtcgttgtgg aaataccctg cagcagtcca
      301 cctgaaccat tcagctctgt agccagacgg gagggtatga ccatcagcat gtcacagggc
      361 tgcgcctgtt ccgggtaatc cgacaaccag ctccagcagg cttctgcatc aatgtgttca
      421 ctcagcccgg ccagaccaaa ccagtcagaa tactgacggc tcatcagcgt ggtgatgatc
      481 tccctggcat tatccggcag attaccccag cgaacagcgc cgatacatga c
//
LOCUS       DAZKGF010000532          529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZKGF010000532 DAZKGF010000000
VERSION     DAZKGF010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.535"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            62..517
                     /locus_tag="ACHOQV_005408"
     CDS             62..517
                     /locus_tag="ACHOQV_005408"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFP9633599.1"
                     /translation="MTTRKSGFNWYVLEFCSARTARVFSHLERLGVTFWCPLTCSHYR
                     RSDKNCFRRKEVPLFPGYVFIKIDFNKTHSTTVTSVPWVKSFVSFGGEPVPVCEEIIL
                     GLRRRETNYWGEYSPIRGIPHELAEILLENNPMKRSFLFFKYLSNTQNR"
BASE COUNT          152 a           88 c          129 g          160 t
ORIGIN      
        1 caaaaaaaca gggctacgga tacttcatga ctgatcgggg atccccgagg cggaggtgtg
       61 catgacgact aggaaaagcg gcttcaactg gtatgttcta gagttttgtt ctgcacgtac
      121 agcacgagtg ttttctcatc tggaaaggtt aggggtaaca ttctggtgtc ccctgacttg
      181 ttcccattat cgtagatcag ataaaaattg tttcagacgg aaagaagtgc cgctgtttcc
      241 tggatatgtg ttcataaaga tcgatttcaa taagacccat agcacaactg ttacgtcagt
      301 tccttgggta aagtcatttg tgtcatttgg tggtgagcct gttcctgttt gcgaggaaat
      361 cattctggga ttgagaagaa gagagactaa ttattggggg gaatattctc ctatacgcgg
      421 aatacctcat gaattggctg aaatcttgtt agaaaataat ccgatgaaaa gaagtttttt
      481 attttttaaa tatttaagca atacgcagaa taggtagttg aaaatcttg
//
LOCUS       DAZKGF010000533          525 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZKGF010000533 DAZKGF010000000
VERSION     DAZKGF010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.536"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..163)
                     /gene="yciX"
                     /locus_tag="ACHOQV_005409"
     CDS             complement(<1..163)
                     /gene="yciX"
                     /locus_tag="ACHOQV_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFP9633600.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQQK"
     gene            complement(166..306)
                     /gene="ymiA"
                     /locus_tag="ACHOQV_005410"
     CDS             complement(166..306)
                     /gene="ymiA"
                     /locus_tag="ACHOQV_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFP9633601.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          155 a          121 c          102 g          147 t
ORIGIN      
        1 gtttctgctg atcatcttca gcaaacagtt ctataaaggc ttgctgctgc gatgttagtt
       61 gccagtcgtc tggaataacg acggaacatt cccgtttcgt ttctgtttca ttgtcactat
      121 gctgacataa tggtgacgac tccagttgct cctgaccgac catagttatc cccacacttt
      181 ccaaatcagc agtgcgacaa ccacccagaa aagagcagaa ccaagaaata ccgccagcca
      241 ggctttgcgt tttaattcag ggtcccggcg gggttcctga tttccagaag gcattgctaa
      301 cctcatataa tcgccatcac ttatcagcaa gacgttgaac gattggtgta attaatcata
      361 cgatgagaca aatcctaaag aaactttagt caaaaatcca gcgaatttac gaatcgtctc
      421 cagtgaataa ttcaatcttt tgacctgaaa tagatatttg aaaactcgag tttgcgaggc
      481 gggtaattag acactacttt acaggtaatt taccctataa atatg
//
LOCUS       DAZKGF010000534          523 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZKGF010000534 DAZKGF010000000
VERSION     DAZKGF010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.537"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          147 a          105 c          124 g          147 t
ORIGIN      
        1 atctggaaag cagatcacag taaataaaaa aacctgctgg tgaggcaggt tcttttattt
       61 actacaatga cgctaacttg ctgattgtgc aacatttcgt accaacgaat aaattataac
      121 catatccaca ctccattgca attcagtgaa tgagatttat aatcctgccc gttgggaggt
      181 tgtgccggat cggaccaatt ttaagtcctg gctgctggac gggtggtgcc gcaggcggtg
      241 ccctgatccg gagagcaacg tttcgtacca acatacgtaa accagtatcg acaggcagtg
      301 taagggtaag gtgctggtgt tgcgctcctt aataaggaga gcggatggac acgctgacac
      361 aaaagttaac cgtgctcatt gctgtactgg agctattagt ggctctgtta cggttgattg
      421 atttgttgaa gtaacgggca aattgaaacg catctaaggg ggaataccgt ttcccccttt
      481 aataaaatga ttactgtttt tattaattca tcatataact cat
//
LOCUS       DAZKGF010000535          523 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZKGF010000535 DAZKGF010000000
VERSION     DAZKGF010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.538"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            240..>523
                     /locus_tag="ACHOQV_005411"
     CDS             240..>523
                     /locus_tag="ACHOQV_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP9633602.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGTEESISLT"
BASE COUNT          147 a           93 c          138 g          145 t
ORIGIN      
        1 gaataatttt ctgcattttt gtattactcc tggatataag ttttgataaa tgctgatttt
       61 gcgatatgca tcagaataaa gatctggtta ttggcattaa aacgatcgat atgtctgtca
      121 tattgagaat ttgcagatct tcgtcctata atgggtgaaa ttaaatcagt gcacaagggg
      181 aggccttctg tggcgatggc gatgggctcc ctgggatgca ggtttcacag gagaacgtca
      241 tgagcagttc gtttcagaat gagatcccga aggcaagggt gaatctgaaa ttagacctac
      301 atacaggtgg tgggcagaag aaagtggagc ttccgctcaa actgctggtt gccggtgatt
      361 tcagtaacgg acaggaagag cgcccgctgt cagagcgcat aaaaatggat gttaataaaa
      421 ataactttaa tgctgttctg gctgatatct cgccgtcagt acgcctgaat gtggagaaca
      481 cacttgccgg tgatggtact gaagagagca tcagcctgac ttt
//
LOCUS       DAZKGF010000536          520 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZKGF010000536 DAZKGF010000000
VERSION     DAZKGF010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.539"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>520)
                     /locus_tag="ACHOQV_005412"
     CDS             complement(<1..>520)
                     /locus_tag="ACHOQV_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP9633603.1"
                     /translation="QGRYRVKMDFDREETEPGFGYLWLRMAKPCAGETLGWHTPLIDG
                     TEVAIAYSNGDIDLPYIAYALHDAEHPDLVSRDNHTRNILRTPANNKLRMEDRRNEEH
                     IKLATEYGKTQLNSGHLVDSQGQLRGKGTELRTDEWGTLRAGKGLFVAADAQAKAQGE
                     ALDMDAALKEID"
BASE COUNT           80 a          172 c          132 g          136 t
ORIGIN      
        1 ctgtcgattt ccttcagcgc ggcatccatg tccagtgcct cgccctgtgc tttcgcctgc
       61 gcatccgcag caacaaaaag tcccttccct gctctcaggg ttccccactc atcggtacgc
      121 agctctgtgc ctttaccccg cagttgcccc tggctgtcca ccagatgtcc gctgttaagc
      181 tgtgtcttgc cgtattccgt agcgagtttg atgtgctcct cattgcgtct gtcttccatc
      241 cgcagcttgt tgttcgcagg cgttctgagg atgttccggg tgtgattgtc ccggctcacg
      301 aggtcaggat gctcagcgtc gtgcagtgca tacgcgatgt agggcaggtc aatatcgcca
      361 ttgctgtatg caatcgccac ttcggtgcca tcaatgagcg gcgtatgcca gcccagcgtt
      421 tcacctgcac agggttttgc catccgcagc cacaggtagc cgaatccggg ttccgtctcc
      481 tcccggtcaa agtccatctt cacccggtaa cgcccctgcc
//
LOCUS       DAZKGF010000537          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZKGF010000537 DAZKGF010000000
VERSION     DAZKGF010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.540"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(171..>514)
                     /locus_tag="ACHOQV_005413"
     CDS             complement(171..>514)
                     /locus_tag="ACHOQV_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP9633604.1"
                     /translation="FRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKL
                     HWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNY
                     LASVLAGSGLS"
BASE COUNT          125 a          119 c          101 g          169 t
ORIGIN      
        1 aatatcttcc caagcatttc catttacttt acctcttgaa attcaaatgg gaatccagtg
       61 aaaggacctt taggcataat atatctttga tatccataaa gctcaccgag cacttgtagg
      121 cttataggcg catttgtatt catagagggg ttgttaaacc cagggaaaga ttacgaaagc
      181 ccgctccctg caaggactga cgccaggtag tttctgtcca tggctgcttt tcgcatctta
      241 cgtcttaacc ctgccttgaa taccttatca ttcgttaaaa tattaatagc gatgtgccgt
      301 atccctgaaa ataattctgc ggcgtttcct cttcttattt tgcagtcgtc ttcattcatt
      361 accacgtcca gacgccagtg cagcttattc tccacgtgcc agtggtttcg gattgctgtg
      421 gcgaactttt ctgcggttaa atcagcagaa ctgatataat atctgactgt catttcgggc
      481 tctttctttt gctctgctat tattgagcga aagg
//
LOCUS       DAZKGF010000538          513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZKGF010000538 DAZKGF010000000
VERSION     DAZKGF010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.541"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..190
                     /locus_tag="ACHOQV_005414"
     CDS             <1..190
                     /locus_tag="ACHOQV_005414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633605.1"
                     /translation="GSVIKGGEGPFLLTPYSRDESARSAGYVSAVCQTIFSSNTPAIP
                     ISATTTPVLITVFAFILT"
BASE COUNT          142 a          104 c          111 g          156 t
ORIGIN      
        1 aggaagcgta attaagggcg gggagggacc attcctgtta acgccctaca gtcgtgacga
       61 atcagccaga agtgctggat atgtgtctgc tgtttgccag acgatttttt caagtaatac
      121 gcctgcaatc ccgattagcg caactacaac gcccgttctg ataactgttt tcgctttcat
      181 tttaacttaa ctgatcaaga cgctgttaac tccatggtcg tacctgctgc tggggacagt
      241 acatgtcagt acttaccaaa caggcttcaa aaaagcaaca gattcgctat atcggaagtt
      301 tcgacggttt cggctctcct ccctgtatga cgactgcgtg tgcagtgctg gcttggttgt
      361 tcaaaagttc aacgaaaaca gtatgaaaaa agaacatcaa aaaaaaatat ttatctggaa
      421 attggtgcta acacgatctg ttattttgtt tttttatcaa tcggtagatc tacgatctat
      481 ttgtgtggtt attttcagtg taaaattcta tag
//
LOCUS       DAZKGF010000539          508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZKGF010000539 DAZKGF010000000
VERSION     DAZKGF010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.542"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            102..>508
                     /locus_tag="ACHOQV_005415"
     CDS             102..>508
                     /locus_tag="ACHOQV_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP9633606.1"
                     /translation="MPQPTLMTDWTCIATSGPTADGREIDPQWLIDAAETYSRNTYTA
                     MLWPCHENDTSYRQYTFNLGEVDALKVETREDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAELIPNFAKSGRDYLMGVAVTDIPASLGTEK"
BASE COUNT          135 a          149 c          128 g           96 t
ORIGIN      
        1 ccgtgttatc cgaatgcaac cgaattaatc cagtagcccc ctgccgtaaa aatcccgaca
       61 ctcaccggag ttcttcagat tttcacgtaa aagaggccac gatgccgcag ccaacgttga
      121 tgaccgactg gacatgcatc gccacatcag ggccgaccgc tgacggtcgc gaaattgatc
      181 cgcagtggct gattgatgcc gctgaaacct attcccgcaa cacctacacg gcgatgctgt
      241 ggccctgcca tgagaacgac accagttacc gccagtacac cttcaatctg ggcgaagtgg
      301 acgcgctgaa agtggaaacc cgcgaggacg gtaaaacccg tctttacgcg cgactggtcc
      361 ctaatcagta cctgattgaa gccaacaggc tggggcaaaa actcttcaca tcagccgaat
      421 taataccgaa ctttgccaaa agcggacgcg actatctcat gggtgtggcg gtgacggata
      481 tccccgccag tctggggacg gaaaaaat
//
LOCUS       DAZKGF010000540          508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZKGF010000540 DAZKGF010000000
VERSION     DAZKGF010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.543"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            102..>508
                     /locus_tag="ACHOQV_005416"
     CDS             102..>508
                     /locus_tag="ACHOQV_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP9633607.1"
                     /translation="MPQPTLMTDWICIATSGPTVDGREIDPQWLTDAAETYSRNTYTA
                     MLWPWHEEEIGHRQYTSNLGEVDSLKAETGEDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAEIVENFARSGRDYLLGVAVTDIPASLGTEK"
BASE COUNT          131 a          148 c          139 g           90 t
ORIGIN      
        1 ccgtgttatc cgaatgcaac cgaattaatc cggtagcccc ccgctgtaaa aatcccgaca
       61 ctcaccggag ttcttcagat tttcacgtaa aagaggccac gatgccgcag ccaacgttga
      121 tgaccgactg gatatgtatc gccacatcag gaccgaccgt tgacggccgc gaaattgatc
      181 cgcagtggct gactgatgcc gctgaaacct attcccgcaa cacctacacg gcgatgctgt
      241 ggccctggca tgaggaagaa atcggacacc gccagtacac cagtaatctg ggtgaagtgg
      301 actcactgaa agcggaaacc ggcgaggacg gtaaaacccg cctctacgca cggctggtcc
      361 ctaaccagta cctgattgaa gccaacagac tggggcagaa gctgttcaca tccgccgaga
      421 tagtggagaa cttcgcccgg agcggacgcg actatctcct gggcgtggcg gtgacggata
      481 tccccgccag tctggggacg gaaaaaat
//
LOCUS       DAZKGF010000541          507 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZKGF010000541 DAZKGF010000000
VERSION     DAZKGF010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.544"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>507
                     /locus_tag="ACHOQV_005417"
     CDS             <1..>507
                     /locus_tag="ACHOQV_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP9633608.1"
                     /translation="ATPESSGSYGFVRLEGDLLITEVAGMSLTTGVYGAAGHSSVDVK
                     DDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASSGNNDFRARGWG
                     WLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRL
                     GSHNDMNFG"
BASE COUNT          103 a          142 c          161 g          101 t
ORIGIN      
        1 gccacgccgg aaagcagcgg cagctatggc ttcgtccgtc tggagggtga cctgctcata
       61 acagaggtcg ccggtatgtc actgacgact ggggtgtatg gtgctgcagg ccattcttcc
      121 gttgatgtta aggatgatga cggctcccgc gccggcacgg tccgggatga tgccggcagc
      181 ctgggcggat atatgaacct gacacacacc tcctccggcc tgtgggctga catagtggca
      241 cagggaaccc gccacagtat gaaagcgtca tcgggcaata acgacttccg cgcacggggc
      301 tggggctggc tgggctcact ggaaaccggt ctgcccttca gtatcactga caacctgatg
      361 ctggagccac aactgcagta cacctggcag ggactttccc tggatgacgg ccaggataac
      421 gccggttatg tgaagttcgg gcatggcagt gcacaacatg tgcgtgccgg cttccgtctg
      481 ggcagccaca acgatatgaa ctttggt
//
LOCUS       DAZKGF010000542          506 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZKGF010000542 DAZKGF010000000
VERSION     DAZKGF010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.545"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..390
                     /locus_tag="ACHOQV_005418"
     CDS             <1..390
                     /locus_tag="ACHOQV_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFP9633609.1"
                     /translation="MKNLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSE
                     KQLTVTSSEDEIIISTPETLTLNGGGSYLRLSKNGIEHGSSGEFIVKTSDYLVPGSGV
                     DLPNEAPGFSLTDITQESKISSKSFND"
BASE COUNT          141 a          111 c          141 g          113 t
ORIGIN      
        1 atgaaaaatc tgtcggtaac ggtggaaaaa gcccttggtc tgtttgtgca taaagacggg
       61 gcgaagctgg tagccaacca gggagatatt gagattcagg ctcagcacaa cacgatggcg
      121 ctgttctctg aaaaacagct gacggtgacc agtagtgaag acgaaattat tatcagcacc
      181 ccggaaactc tgacgctgaa cggtggtggg tcttacctga gactgagtaa gaacgggata
      241 gagcacggct catcgggtga gtttattgtg aagacgtctg actatctggt gcctggttct
      301 ggcgtagatc tccccaacga agcacctggt ttcagcctga cggatatcac acaggaaagc
      361 aaaatcagca gcaagtcttt taacgactga cagggagtat gaatcgtgtc tgtattaccc
      421 aaaatcgtct ggccgatccc ctccagccgt cggggaagtg agttcaaaaa tcaggaagag
      481 atcctctctc atttgggggg agaatc
//
LOCUS       DAZKGF010000543          506 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZKGF010000543 DAZKGF010000000
VERSION     DAZKGF010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.546"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..226
                     /locus_tag="ACHOQV_005419"
     CDS             44..226
                     /locus_tag="ACHOQV_005419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633610.1"
                     /translation="MIFNWNVLFYVSLEKFPVGNPYYGEIPGIPVDEEAKFFNPFPPR
                     AFENKPLYTEEITKYD"
     gene            219..>506
                     /locus_tag="ACHOQV_005420"
     CDS             219..>506
                     /locus_tag="ACHOQV_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9633611.1"
                     /translation="MIKGIMFAFLAAFSWGAAIVMSKKGLENMDAGELFLWQVGSAAM
                     LSWFVLAISRKKLPVTKKSILAYSTGIFEPFLAYTFTLYGLKFISAGITAVI"
BASE COUNT          152 a           82 c          100 g          172 t
ORIGIN      
        1 ttaggacgcc atcataatcc atgatgttga tagtaatgat cctatgattt ttaactggaa
       61 tgtattgttt tatgtctcac tggaaaagtt tcctgttggc aatccttatt atggagaaat
      121 tccaggtatt cctgtggatg aagaagcaaa gttttttaat ccatttccac caagagcgtt
      181 tgaaaataaa cctctttata ctgaggagat aactaaatat gattaaggga attatgtttg
      241 catttcttgc tgctttttca tggggagcag caatagtgat gagcaaaaaa ggtctcgaaa
      301 acatggatgc cggagagttg tttttatggc aagttggtag tgctgcaatg ttatcatggt
      361 ttgttctcgc aattagcaga aaaaaactcc cggtaacaaa aaaatcaatc cttgcctatt
      421 caacaggaat ttttgagcca tttctagcgt atacattcac tctctatggc ctaaaattta
      481 tttctgctgg tataacagct gttatt
//
LOCUS       DAZKGF010000544          501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZKGF010000544 DAZKGF010000000
VERSION     DAZKGF010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.547"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>501
                     /locus_tag="ACHOQV_005421"
     CDS             <1..>501
                     /locus_tag="ACHOQV_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP9633612.1"
                     /translation="RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRL
                     TTIQNDRTRIQTVYQPGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFP
                     PVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQIQNQMDPVYTPARKIHLYHCDH
                     RGLPLAL"
BASE COUNT          104 a          149 c          173 g           75 t
ORIGIN      
        1 cgctatcttt acgacccgct gggccgcagg gtggcaaaac gggtatggcg gcgtgaacgg
       61 gacctgacgg gctggatgtc gctgtcacgg aaaccggaag tgacctggta cggctgggac
      121 ggcgaccggc tgaccacgat acagaacgac agaacccgca tccagacggt ttatcagccg
      181 gggagcttca cgccactcat cagggtcgaa accgccaccg gagagctggc gaaaacgcag
      241 cgccgcagcc tggcggatgc ccttcagcag tccggcggcg aagacggtgg cagtgtggtg
      301 ttcccgccgg tgctggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg
      361 gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtgga gcagatacaa
      421 aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat
      481 cgcggcctgc cgctggcgct t
//
LOCUS       DAZKGF010000545          501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZKGF010000545 DAZKGF010000000
VERSION     DAZKGF010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.548"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>501
                     /locus_tag="ACHOQV_005422"
     CDS             <1..>501
                     /locus_tag="ACHOQV_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
                     /protein_id="HFP9633613.1"
                     /translation="FNGYFSDVELSKGNSALTEKVNRDYFSKPADDYFLNNHIISYDP
                     SKNQWKNLGTTPFPGTAGSSVIFAEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSE
                     LPPVASPEGVSGAYASVIDGNIFLAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKEIYQ
                     LIKNDWK"
BASE COUNT          153 a           87 c          123 g          138 t
ORIGIN      
        1 tttaatggat acttttcaga tgtcgagtta tcaaaaggga attccgcgct gacagagaaa
       61 gtcaacaggg attattttag taaacctgct gatgattatt ttttaaataa tcatataatt
      121 tcttatgacc cgtcaaaaaa ccagtggaag aacctgggaa cgactccatt tccgggaact
      181 gctggttcat cggtgatttt tgcagaacag caaatttata tactaggagg ggagagaaaa
      241 ccggggcttc gttctgttag atcctggacg ggggagcttt cgcatgatcg aattaaatgg
      301 tctgaacttc caccagtagc ttcacccgag ggggtgtcag gagcatatgc ctctgttatt
      361 gatggaaata tctttctggc cggtggtgca tatttcccgg gggcggcaga aaagtatagc
      421 aatggtgaat actggtctca taaaggactg gataaagcct attcaaaaga gatatatcag
      481 ctaataaaaa atgactggaa a
//
LOCUS       DAZKGF010000546          494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZKGF010000546 DAZKGF010000000
VERSION     DAZKGF010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.549"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            36..>494
                     /locus_tag="ACHOQV_005423"
     CDS             36..>494
                     /locus_tag="ACHOQV_005423"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9633614.1"
                     /translation="MGTLCEGKGQKIAIEAMHNLIREGYLCHLKIIGNNRVPYASYLN
                     KIVADYNLSDYVEFMGFRHDLDQIRLDNDVCLIPSLSEAFGRVTIESMAAGMIVVASD
                     SGASKEIINDGINGFLFSSGSVSDLTSVLKKILDVESNNLECIRKRALVDS"
BASE COUNT          149 a           60 c          112 g          173 t
ORIGIN      
        1 atgctaattc ggctaggctg cggaagttca ttattattgg cacattgtgt gagggcaaag
       61 gtcaaaaaat agccatagag gctatgcata atctgattcg tgaaggatat ttatgtcact
      121 taaaaataat tggaaataat agagttccat atgcaagtta tcttaataaa attgttgctg
      181 attataattt gagtgattat gtagagttta tgggatttag gcatgatctt gatcaaataa
      241 ggcttgacaa cgatgtttgc ttaattccct ctctttctga agcttttggt agagttacca
      301 ttgaatcaat ggctgcaggt atgatagttg ttgctagtga ttctggggct agtaaagaga
      361 tcatcaatga tggtataaat ggttttttgt tttcttcagg ttcggtaagt gatcttacta
      421 gcgtacttaa aaaaatcctt gatgtagagt ctaataattt agaatgtata aggaaaaggg
      481 ctttggttga ctcg
//
LOCUS       DAZKGF010000547          493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZKGF010000547 DAZKGF010000000
VERSION     DAZKGF010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.550"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>493
                     /locus_tag="ACHOQV_005424"
     CDS             <1..>493
                     /locus_tag="ACHOQV_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9633615.1"
                     /translation="ALIFQGHPVIHAGQTVSASQSDWENREFSLNNLNLNNADFSLSR
                     NAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKSAAAGTSSYTGHITLEQ
                     KSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLNIHKGGALTAQGGLFTS
                     GSID"
BASE COUNT          136 a          106 c          118 g          133 t
ORIGIN      
        1 gctcttatct tccagggaca cccggttatt catgcagggc aaactgtttc tgcatcgcag
       61 agtgactggg agaacaggga gttctcactc aacaatctga atcttaataa tgcggacttc
      121 agtctgtccc gtaatgcatt tatgaacggg aatatcaggg ccgttaacca gagcactgtt
      181 attatcggcg gagatacagt ctttactgat aaaaatgacg gaacaggtaa tgatgtcatc
      241 agtgttgaag ggaaatctgc tgccgcagga acatcctcct atacagggca tatcactctg
      301 gagcaaaaat cagcactgga tatccgcgat aattttcgtg gcggggttac gtctgaagac
      361 agtcatatca atgtttcttc atcttcagtc ctgttctcag atgcatcgtc atttataaac
      421 agctccctga atattcataa aggaggtgcg ctgaccgctc agggagggct gtttacaagt
      481 ggaagcattg ata
//
LOCUS       DAZKGF010000548          493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZKGF010000548 DAZKGF010000000
VERSION     DAZKGF010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.551"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            209..>493
                     /locus_tag="ACHOQV_005425"
     CDS             209..>493
                     /locus_tag="ACHOQV_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633616.1"
                     /translation="MSEVKKHVSYSLTVAAMLSAAIVCQTAHADVNTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIR"
BASE COUNT          153 a          117 c          102 g          121 t
ORIGIN      
        1 tttccgtaat ctgatgcatc aggggtatgc ccaccacaaa taactctgta cagcaacaga
       61 aaaatttccc tggtcatatg atgaattaat catcgccatc acaccggatg cttctcatcc
      121 tgtcctcaga aaggcaggtg gtctcctgtc tgccgcgctt atccaggata ccgggacctg
      181 aaaggtcata atctggcagg aaaaaataat gtccgaagta aaaaaacacg tttcttattc
      241 attaacagtt gcagctatgc tgtcagccgc catcgtctgt cagacagcac atgctgatgt
      301 caacacaccg aacatcaccg gagaaaaaaa caatacaaca acacgattct ccggaaatat
      361 ttacggcagt gaaaatactg tcacatcaga gtcaaacagt ctggttgtgg gtaacagtaa
      421 ttccgtggag ggagggtata acaacattgt tattggtaat tcctccacgg tcaaagcttc
      481 cggtttgatc aga
//
LOCUS       DAZKGF010000549          492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZKGF010000549 DAZKGF010000000
VERSION     DAZKGF010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.552"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(2..295)
                     /locus_tag="ACHOQV_005426"
     CDS             complement(2..295)
                     /locus_tag="ACHOQV_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9633617.1"
                     /translation="MFKEKLEDYTEEEFLNFLGGLRSTMKDGKPLKGKELEMYWDSLV
                     DHFIEITQHPSGSDLIFYPKSQGDDKPENILKIVKEWRRSQGLPLFKDSKQSR"
     gene            complement(298..>492)
                     /locus_tag="ACHOQV_005427"
     CDS             complement(298..>492)
                     /locus_tag="ACHOQV_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP9633618.1"
                     /translation="NQTRIKQGLTAKAPLEGWRYGPKEIVKKFQIHHRVAIEYGGSVY
                     DIDNLRIVTPRLHDEIHYRR"
BASE COUNT          127 a          101 c           84 g          180 t
ORIGIN      
        1 attatctact ttgtttagag tctttaaaca agggtagccc ctgtgaacgc cgccattctt
       61 tgacaatttt caggatgttt tcaggcttat catctccttg acttttaggg tagaaaatta
      121 gatcgctccc tgacggatgc tgtgtaattt caataaaatg atctactaat gaatcccagt
      181 acatttctag ctccttacct tttaagggct ttccatcttt catagttgat ctcaatccgc
      241 ctaaaaaatt aagaaattcc tcctcggtat aatcttcgag cttttcttta aacatattta
      301 tctcctgtag tgaatctcat cgtgtagtct gggggtaaca attcgtaaat tatcaatatc
      361 gtatacgctg ccgccatatt caattgcaac acgatgatgt atctggaatt ttttaacgat
      421 ttctttaggg ccataacgcc aaccttcaag aggtgctttt gcggttaatc cttgttttat
      481 acgtgtttgg tt
//
LOCUS       DAZKGF010000550          488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZKGF010000550 DAZKGF010000000
VERSION     DAZKGF010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.553"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..449)
                     /locus_tag="ACHOQV_005428"
     CDS             complement(<1..449)
                     /locus_tag="ACHOQV_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9633619.1"
                     /translation="MRGSVIIPVALHALVAVSTLLWLLWYFIPAGNVFNGLATLLILL
                     LAGWSVFKNSRSEKQKLTSDVTLTDAEPALSDTRGPVVLVCGDMPEALFQDGPLRKTA
                     RGWWLRVGDVSRLTDVVRSIQTQFPRQVGQLSVMYRCLPDCHHDEAVL"
BASE COUNT          129 a          141 c          128 g           90 t
ORIGIN      
        1 agaaccgctt catcatgatg acagtctggc aggcagcggt acatcacaga gagctgaccg
       61 acctggcgcg ggaactgcgt ctgtatgctg cggacaacgt cagttaagcg actgacgtca
      121 ccgacccgca gccaccatcc ccgggctgtt ttacgcagcg gaccgtcctg aaacaacgct
      181 tccggcatgt ctccacatac cagtacaaca gggccccggg tgtcggataa tgccggttca
      241 gcatccgtca gggtaacgtc agatgtgagc ttttgctttt cactccggct gtttttaaaa
      301 acactccagc ctgccagaag aaggatcagc agagtagcca gaccgttaaa aacgttgcct
      361 gccgggatga aataccacag caaccacagc aacgtggaaa cggccaccag ggcatgcagg
      421 gctaccggaa tgatgacaga tccacgcatc agccatgcaa ctccggtatt tgtgatgaca
      481 aaagagac
//
LOCUS       DAZKGF010000551          488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZKGF010000551 DAZKGF010000000
VERSION     DAZKGF010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.554"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>488
                     /locus_tag="ACHOQV_005429"
     CDS             <1..>488
                     /locus_tag="ACHOQV_005429"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9633620.1"
                     /translation="QRCSTGSTIKNVSLKMMRDFRFLMPESKEEQEKVVRIIQKIDEL
                     KRLNNAQNQTLEQMFQALFKSWFVDFDPVIDNALDAGNPIPETLQSRAELRQNVRNST
                     DFKPLPAEIRSLFPSEFEETELGWVPKGWESETFDSFCDLIQSGGTPSRKETSFWDGG
                     TI"
BASE COUNT          161 a           87 c          113 g          127 t
ORIGIN      
        1 caaaggtgtt caactgggag tacaattaaa aacgtatcac taaaaatgat gcgtgatttc
       61 aggtttttaa tgcctgaatc aaaagaggag caggaaaagg ttgttcgtat aatacaaaaa
      121 attgatgaac taaaaagatt aaataatgct caaaatcaaa ccctggaaca aatgttccaa
      181 gccctgttca aatcctggtt tgtagatttt gatccggtaa ttgataacgc tctggatgca
      241 ggaaatccaa tcccggaaac cctgcaatct cgtgccgagt tgcgtcaaaa tgtacgtaat
      301 agtacagatt tcaaaccgct tccggcggaa attcgctcgc ttttcccaag tgaatttgaa
      361 gaaacggagt tgggttgggt gccaaagggt tgggaaagtg aaactttcga ttcattctgc
      421 gacttaattc agagtggggg aacgccttca aggaaggaaa cttcattctg ggatggcggt
      481 acaataaa
//
LOCUS       DAZKGF010000552          486 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZKGF010000552 DAZKGF010000000
VERSION     DAZKGF010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.555"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..335
                     /locus_tag="ACHOQV_005430"
     CDS             <1..335
                     /locus_tag="ACHOQV_005430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633621.1"
                     /translation="KPSASAEGFTQSRNGETAMSTNTPTEINKLFTDPAHIELTQKTL
                     TEITNVLDERISEIDKDKHFATYMALQMQSMAMVTAKQTISELYMKNLRLERELAELR
                     AQSGQFSA"
BASE COUNT          161 a          129 c          106 g           90 t
ORIGIN      
        1 taaaaccatc cgcatctgcg gagggcttca ctcaatccag aaacggagaa acagctatga
       61 gcaccaatac acctacagaa ataaacaaac tatttaccga cccggcgcat attgaattga
      121 cgcaaaaaac gctgactgaa ataaccaatg tactggatga aagaatatca gaaatagata
      181 aagacaagca ttttgccacc tacatggccc tgcaaatgca aagcatggca atggtgacag
      241 caaaacagac catcagtgaa ctctatatga aaaacctgcg cctcgagcgc gaactggcag
      301 aactccgggc acagtccggc cagttttcag cgtgaggggc aggacgatga acagcatatt
      361 taccgccaca agacgaaccc tgctgacgca tttcaccgat gccgcaggcc gcgagttcat
      421 ggtggaaagt tatttgatca caacgaccac gccgtgcccg tcagacgctg attatctcta
      481 catcca
//
LOCUS       DAZKGF010000553          484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZKGF010000553 DAZKGF010000000
VERSION     DAZKGF010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.556"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..436
                     /locus_tag="ACHOQV_005431"
     CDS             <1..436
                     /locus_tag="ACHOQV_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFP9633622.1"
                     /translation="IDRIKRIEKYKHRIHLYNKDAIEFMTELDECMPKKSMFCIDPPY
                     FDKGSLLYTNFYNAEDHKKVSEAILKLKHPWLLTYDNALEINELYNNCARYTYPLNYS
                     AATKRVGTELLITQDGIHIPNNLGITRVINKEKVMSTDEVSI"
BASE COUNT          190 a           68 c           78 g          148 t
ORIGIN      
        1 aatagacagg attaaaagaa tagaaaagta caagcataga attcatttgt ataataaaga
       61 tgctatcgaa tttatgaccg aacttgatga atgcatgcca aaaaaatcaa tgttttgtat
      121 tgatcctcct tactttgata aaggctcatt gctctacact aacttttata atgctgaaga
      181 tcataaaaaa gtgtccgaag ctattttaaa actaaagcat ccatggcttt taacttatga
      241 taatgcatta gaaattaatg aattatataa taattgtgca cgctatacgt acccattgaa
      301 ttattctgct gcaacgaaac gagttggaac agagttactt ataactcaag atggcataca
      361 tattccaaat aatttaggaa ttacaagagt tatcaataag gaaaaagtaa tgagtacgga
      421 tgaagtgtca atataaattt aatatgctca cagctactga gagataactg atcttatatg
      481 gtaa
//
LOCUS       DAZKGF010000554          484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZKGF010000554 DAZKGF010000000
VERSION     DAZKGF010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.557"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(143..>484)
                     /locus_tag="ACHOQV_005432"
     CDS             complement(143..>484)
                     /locus_tag="ACHOQV_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP9633623.1"
                     /translation="FRSIIAEQKKEPEMTVRYYISSADLTAEKFAIAIRNHWHVENKL
                     HWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNY
                     LASVLAGSGLS"
BASE COUNT          120 a          111 c           91 g          162 t
ORIGIN      
        1 ttaggtaata aggaatatct caattattga atatttagtg cgaattattt agtacaaaaa
       61 agcagcgttg cgtgatcttt ccctgccctt tattcataaa ctcatggata tggtgatcaa
      121 cggtggtctg atcagggcaa gattacgaaa gcccgctccc cgcaaggact gacgcgagat
      181 agtttctgtc catggctgct tttcgcatct tacgtcttaa ccctgccttg aataccttat
      241 cattcgtcaa aatattaata gcaatgtgcc gtatccctga aaataattct gctgcatttc
      301 ctcttcttat tttgcagtcg tcttcattca ttaccacgtc cagacgccag tgcagcttat
      361 tctccacgtg ccagtggttt cggattgcta tggcgaactt ctctgcggtt aaatcagcag
      421 aactgatata atatctgacc gtcatttctg gctctttctt ttgttctgct attattgacc
      481 gaaa
//
LOCUS       DAZKGF010000555          483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZKGF010000555 DAZKGF010000000
VERSION     DAZKGF010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.558"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(67..>483)
                     /locus_tag="ACHOQV_005433"
     CDS             complement(67..>483)
                     /locus_tag="ACHOQV_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9633624.1"
                     /translation="EQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRAW
                     RVKDISNSLYMSESLLKRKLQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGY
                     ASTPYFISVFREYFGITPHQMMINQSGFIESRIAIK"
BASE COUNT          154 a           90 c           98 g          141 t
ORIGIN      
        1 ggagtgataa acagaaactg ataatgaagc ataagaatac ttttattatg cgctgactga
       61 catactttac ttaattgcga ttctgctctc aataaatccg gactgattaa tcatcatctg
      121 atgaggtgtt atgccaaaat attctctgaa aacgctgata aaatacggtg tgctggcata
      181 accgcaccgt attgcgattt gattcactga cagtttactg gaaataagtt gtcgggcgtt
      241 atgcatgcgt atatccagca ggatttggga gaaagaaact ttttcctcct gaagttttct
      301 tttcagtaaa ctctcgctca tgtacagaga attactgata tctttaactc tccatgcccg
      361 tgatggactg gaacagataa tatacctgac tttaagcgca acagagttta tgcatctgaa
      421 tagcagaaac atgaaacctc tgtgtgtgga aaaaatggaa aggcaggcaa aaaacatctg
      481 ctc
//
LOCUS       DAZKGF010000556          479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZKGF010000556 DAZKGF010000000
VERSION     DAZKGF010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.559"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          142 a          103 c           95 g          139 t
ORIGIN      
        1 cattccggca agttatgaca gaattactga aaaccgttat ctgtagttat tgtagtttct
       61 gatatatgct catccacccg gaaatacgac atggtcctga ctggcgttca gttcaaagtg
      121 taatttatta ttcacacact gagactgaaa aacggatggc gtggatgagt acacagcctg
      181 aagagtataa aaggaacagc tccggattgt tacccgggat tactatagtt caccacacaa
      241 ccgtaaacct ggttgtgtgg tgaaacagta acatattaag aatattgtgt taccacctgt
      301 tttctcagga aacggattgc aggagaggta tactcattca tcttcccgga gttttgttaa
      361 tcactgcata atcaaacgga ccaatacatt ctttgctcgc atccgtaaaa atgtcgccac
      421 aatatcatca gtcagtcaac tcattaccat ctgttttccg ggtccgtagt tactacagt
//
LOCUS       DAZKGF010000557          472 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZKGF010000557 DAZKGF010000000
VERSION     DAZKGF010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.560"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          185 a           85 c           73 g          129 t
ORIGIN      
        1 accaataaca tcataaacta caaacttgct ctcagtgagt ctgaaaaaac gtgtacaagg
       61 ccaacatata cctgtaaccc aataaactta cacgcactca tttaatcggt taaaaacaac
      121 tcatgttcaa gatatgaacg ttaacgggta cacggtacca gaataaaata taccattgat
      181 ggataatttt cattttgttt aacatacaag ataatcagaa acgaacaaat ataatcagga
      241 attcactctg ttaatttcat tgtttatcct gcgctgttaa acatgacgga atcatggata
      301 ccgtatcaaa gcgtaagcat tcagttaatc agtgatgaca gagaattgat gttcacaaga
      361 acagttaaaa cacttcagat aaacaatcat cgttaagtca atacagttca tgatagataa
      421 gattaaaatc aattactgtt gcagtgaaga ctgataacag atggatatca gc
//
LOCUS       DAZKGF010000558          470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZKGF010000558 DAZKGF010000000
VERSION     DAZKGF010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.561"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>470
                     /locus_tag="ACHOQV_005434"
     CDS             <1..>470
                     /locus_tag="ACHOQV_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFP9633625.1"
                     /translation="NTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAAVTSLP
                     VLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQWIKAG
                     GTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVHGG"
BASE COUNT          107 a          114 c          160 g           89 t
ORIGIN      
        1 aatacctgct acaggctggt atggaatcac attacgggcg ctttcgtggt tgcctccgaa
       61 ctggcccgcg cacggggtaa acgtggcggt gtggcggttg cactgtctct tgccgcggtc
      121 acgtcactcc cggtgctggc tgctgacatc gttgtgcacc cgggtgaaac agtgaatggc
      181 ggaacactgg taaaccatga caaccagttt gtatccggaa cagctgatgg cgtgactgtc
      241 agtaccgggc ttgagctggg gccggacagt gacgaaaaca ccggcgggca atggataaaa
      301 gcgggtggca caggcagaaa caccactgtc accgcaaatg gtcgtcagat tgtgcaggca
      361 ggaggaactg ccagtgatac ggttattcgt gatggcggag ggcagagcct taacggactg
      421 gcggtgaaca ccacgctgga taacagaggt gagcagtggg tacacggggg
//
LOCUS       DAZKGF010000559          468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZKGF010000559 DAZKGF010000000
VERSION     DAZKGF010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.562"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>468
                     /locus_tag="ACHOQV_005435"
     CDS             <1..>468
                     /locus_tag="ACHOQV_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP9633626.1"
                     /translation="EKGDPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPRGEPGP
                     AGPRGERGETGPQGPRGEPGPAGSAANVADATTAQKGIVQLSSATDSDDETKAATPKA
                     VKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWKTREFV"
BASE COUNT          129 a          106 c          175 g           58 t
ORIGIN      
        1 cgagaaaggt gatccgggag gtcctgcagg cccgcaaggt cctaaaggtg aacgaggaga
       61 agccggacca cagggaccga tgggagcacg aggtgagcgt ggggagactg gcccccgggg
      121 tgaacctggt cctgcaggtc cgagaggcga acgaggagag accggacctc agggacctcg
      181 tggagagcca ggtccggcag gcagcgctgc aaatgtggct gatgcgacga cggcacagaa
      241 gggaattgtg cagttaagca gcgcaacgga cagtgatgat gaaacgaagg cagccacccc
      301 gaaagcggtg aaagcggcaa tggacaaagc ggacggatgc ctggagaaag cgaaaaacgg
      361 tgacgatatc ccggataagg tgaagtttct gaacaccgtg ggagcagcca gagtatacgg
      421 gcgggacatt catacggggg ccggtgaatg gaaaacacgc gagtttgt
//
LOCUS       DAZKGF010000560          468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZKGF010000560 DAZKGF010000000
VERSION     DAZKGF010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.563"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>468
                     /locus_tag="ACHOQV_005436"
     CDS             <1..>468
                     /locus_tag="ACHOQV_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633627.1"
                     /translation="SAIHHQIFKNQLDTETITVSYEFILCQHLITNTEFSYLALPENY
                     NRLFLPNSKNKTNNGLKTLNSEAVGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQ
                     ILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIY"
BASE COUNT          177 a           84 c           82 g          125 t
ORIGIN      
        1 tcggctattc atcaccagat ctttaaaaac caactggata cagaaacaat aactgttagt
       61 tatgaattta tcctctgcca acatcttatc actaacaccg agttcagcta tttggcactc
      121 cctgaaaatt ataaccgctt gtttttacca aattcaaaaa acaaaacaaa taatggttta
      181 aaaacgttaa acagcgaagc tgttggcagg ctacttgctg ctggtggcgt atacaatggg
      241 aacattgaag gtttcagaga tactgcggaa aaactgggtg gagatgcaat caaaggctat
      301 gatcaaatac taaatgaaaa aacagcgggc atagcgatag caacagcatc tattctttta
      361 acaaagcgtt ctaatgttga tacatataca gaaataaata gttacttagg caaacttaga
      421 ggtcaacaaa aacttcttga tggtatagac ataatagaaa taatatac
//
LOCUS       DAZKGF010000561          452 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZKGF010000561 DAZKGF010000000
VERSION     DAZKGF010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.564"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>452
                     /locus_tag="ACHOQV_005437"
     CDS             <1..>452
                     /locus_tag="ACHOQV_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP9633628.1"
                     /translation="VWNKRIPKNDKGIGNIHEYILAYAKNNEISKEFLMRKDGIDDVY
                     QFIEKLKKEKVPLDKAEQQLKKFYSSNGYDRGITLYNGLNEDYRPWGKINMSWPNADS
                     FGPTYEVLHPLTNNPVKIPDRGWRWKEGTFNHIAKRIDGKYADIKKLH"
BASE COUNT          171 a           66 c           94 g          121 t
ORIGIN      
        1 ttgtatggaa taaacgtatc cctaaaaatg ataagggtat tggtaatatc catgagtaca
       61 tccttgctta tgcgaagaat aacgaaataa gtaaagaatt tttaatgcgg aaagatggta
      121 ttgatgatgt atatcagttt attgaaaagt taaaaaagga aaaggtgcca ttagataaag
      181 cagagcagca actcaaaaaa ttctacagct ccaacggata tgatcgtgga attactcttt
      241 acaacggttt gaacgaggat tataggccat ggggtaaaat taatatgagt tggcctaatg
      301 cagattcatt tggacccacg tatgaagtat tgcacccttt gactaataac cctgtcaaaa
      361 tcccagacag aggctggcgt tggaaagaag gcacttttaa tcatattgca aaaagaatag
      421 atggaaagta tgcagatatc aaaaaactac at
//
LOCUS       DAZKGF010000562          451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZKGF010000562 DAZKGF010000000
VERSION     DAZKGF010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.565"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>451)
                     /locus_tag="ACHOQV_005438"
     CDS             complement(<1..>451)
                     /locus_tag="ACHOQV_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9633629.1"
                     /translation="MFPDAVLSLLAPFASTVQGGQRTRRLILLIWLCVLTALGISALN
                     NRDLIRQVSTDLQRWNAVPKGHYRPEAESLAALKQDALLLEQWQRQGVPLRYSLGYYP
                     GQRLWLALQQAIDTRVPPPSPEPEPVPEIVRLDSLALFDAGKWTLKPG"
BASE COUNT          104 a          138 c          125 g           84 t
ORIGIN      
        1 cccggtttca gcgtccattt accggcatca aacagtgcca ggctatcgag tcgtacaatt
       61 tccggcaccg gttctggttc aggggatggt ggtggaaccc gggtatcgat agcctgctgc
      121 agggccagcc acagacgttg tcccgggtaa tagcccaggc tgtaacgtaa aggcactccc
      181 tgacgctgcc actgctcaag cagaagcgca tcctgtttca gtgcggccag cgattccgcc
      241 tcaggacggt agtgcccctt cgggacagca ttccagcgct gtaaatcggt gcttacctgg
      301 cggatcagat cacgattgtt aagcgcagaa atccccagcg cagtcaggac acacagccat
      361 atcagcaaaa tcagacgacg ggtacgctgg cctccctgta ccgtcgacgc aaaaggtgcc
      421 agcagcgaca gaaccgcatc cgggaacatc c
//
LOCUS       DAZKGF010000563          451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZKGF010000563 DAZKGF010000000
VERSION     DAZKGF010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.566"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          123 a           86 c          125 g          117 t
ORIGIN      
        1 ggccgggatg tgaatattta tggtgggcta ttggcgaaaa atatctggaa agtagatcgc
       61 agaaaataaa agaacctgct ggtgaggcag gttcttttat ttactacttt gatgttaagt
      121 tactgatagt gcaacatggc atctcaacga acgaattata accaccgctg gaccgtgttg
      181 caattaagat aatgagattt ataatcctga ctgttgggaa gttgcgccgg atcgaaccca
      241 tttcaagtcc tggctaccgg acgggtggtg ccgcaggtag tgctctgatc cggagtgcaa
      301 catggcgact caacaaacgt aaaccagtgt cgacaggcag tgtaagggta aggtgctggt
      361 gttgcgctcc ttaataagga gagcggatgg acacgctgac acaaaagtta accgtgctca
      421 ttgctgtact ggagctatta gtggctctgt t
//
LOCUS       DAZKGF010000564          450 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZKGF010000564 DAZKGF010000000
VERSION     DAZKGF010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.567"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>450
                     /locus_tag="ACHOQV_005439"
     CDS             <1..>450
                     /locus_tag="ACHOQV_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633630.1"
                     /translation="SLLMLSGCATEALKNEQAETVITAGNKAVAANASFIKQYYASRE
                     NFLVNFYATNPDCALRPDYTIDIGGDGNGGKLCQRSGPNTKQVQLVPFEKEKLLASME
                     VINSMSAYVELLTKDMATTESQQVALLKQALDHANNAQTLLGVSHSK"
BASE COUNT          149 a           90 c           98 g          113 t
ORIGIN      
        1 atcgctgtta atgctttcag gttgtgcaac tgaagcattg aaaaacgaac aagctgaaac
       61 tgttattact gcgggtaaca aagcagtagc ggcgaatgca agtttcatta aacaatacta
      121 tgcttctcgt gaaaacttcc ttgttaattt ctacgcgact aatccggatt gtgccctgcg
      181 acctgattat accattgata taggcggtga tgggaatggc ggaaaattat gccagcgttc
      241 agggccaaat acaaaacaag tacaattagt ccctttcgag aaagaaaaat tactggcctc
      301 aatggaagtc attaatagca tgagcgctta tgtggaactt ctgacaaagg atatggcaac
      361 tacggaaagt cagcaagtgg cattacttaa acaggctctg gatcacgcaa acaacgcaca
      421 aacactactt ggtgtttcac atagcaaaga
//
LOCUS       DAZKGF010000565          450 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZKGF010000565 DAZKGF010000000
VERSION     DAZKGF010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.568"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..302)
                     /locus_tag="ACHOQV_005440"
     CDS             complement(<1..302)
                     /locus_tag="ACHOQV_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HFP9633631.1"
                     /translation="MEKDYLGISSTVLVSLLFGLALVLVNSWFNQPTVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFIKTVIIIQQIIEG"
BASE COUNT          180 a           86 c           59 g          125 t
ORIGIN      
        1 ccctcaataa tttgctgaat gataatgacg gttttaataa agtaaatcaa acaacttaga
       61 aaagccaaac taagtataca gtttgaaaat tgcctacggt cataaatgaa atacaactgc
      121 ataaaaataa atgcaacagt atcgataaaa aataaagcaa tcataaccat cagataggtt
      181 gatcgtggaa caacttcctc aacggttggc tgattgaacc aactattaac caaaaccaat
      241 gcaagcccaa acaataagct cactaataca gtactactaa ttcccaaata gtctttttcc
      301 ataaagctac accaacctgt aggttgtaga tcccttttta ttattaatag atataagaac
      361 tgtactgttg tcaatatgaa aatccaccca caaacaatac cgatgatgat agttgcagaa
      421 tgtaattatt atcactttaa tgctttatac
//
LOCUS       DAZKGF010000566          448 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZKGF010000566 DAZKGF010000000
VERSION     DAZKGF010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.569"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..412
                     /locus_tag="ACHOQV_005441"
     CDS             <1..412
                     /locus_tag="ACHOQV_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFP9633632.1"
                     /translation="SVSSLPQDRAFYRPDLDRIVMPLSSQFDQVADYWTTLLHELVHS
                     TGHEKRLRRVGISTFTGRNSPEYAFEELIAEMGSAFLCAHLGIAGDVQHESYIDGWLK
                     VLKSDKKALFRACRQAREASEYLLTRRTSQECAA"
BASE COUNT           99 a          113 c          130 g          106 t
ORIGIN      
        1 cagcgtctcg tcacttccac aggacagggc attctaccgg cctgatcttg accgtatcgt
       61 gatgccactg tcgtcgcagt ttgaccaggt ggctgattac tggaccaccc tgctgcatga
      121 gctggtacac agtaccggtc atgaaaagcg cctgcggcgg gtggggataa gtacctttac
      181 cggtcgcaac agcccggaat atgcgtttga agagctgatt gccgagatgg gcagtgcttt
      241 tctgtgtgca catctcggga ttgccggtga cgtacagcac gaaagttata ttgacggatg
      301 gcttaaggtt ctgaagtctg acaaaaaagc gctgtttcgc gcctgccggc aggcacgtga
      361 agcatcagag tatttactga cgcggcgaac ttcgcaggag tgcgcagcat aatttttact
      421 accggtggcg gttataacct gaccgcca
//
LOCUS       DAZKGF010000567          447 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZKGF010000567 DAZKGF010000000
VERSION     DAZKGF010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.570"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>447)
                     /locus_tag="ACHOQV_005442"
     CDS             complement(<1..>447)
                     /locus_tag="ACHOQV_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFP9633633.1"
                     /translation="LWHMHPIMFLAFLIQPNEDIRILRLRAFLRMIRIGEGTIQEDGY
                     RTMFTGVKFTDFSKHPNIRHEANGVVSTAAGAYQFLYGTWRNLQRRYSFPDFSPSNQD
                     LGCIALIAGRKALDVVMQGKISEAIHLCRIEWASLPGSPHGQPTAN"
BASE COUNT          135 a           97 c           81 g          134 t
ORIGIN      
        1 ttatttgcag ttggttgacc atgcggtgaa ccgggtaaac tggcccactc aatacgacat
       61 aaatgtatgg cttcactaat cttaccctgc attactacat ctaatgcttt tcgacctgca
      121 atgagcgcta tacaaccgag atcttgattg ctcggactga aatctggaaa tgaatatcta
      181 cgttgcaaat ttctccatgt tccgtataaa aattgataag ctccagcagc agtcgaaaca
      241 acaccattag cttcatgcct gatattgggg tgtttactaa aatctgtaaa ttttacacca
      301 gtaaacatcg ttctgtatcc gtcttcctga attgtccctt caccgatgcg gatcattcgt
      361 agaaatgcac gtaaacgaag aattctaata tcttcattag gttgaattag gaaggctaaa
      421 aacattattg gatgcatatg ccacaac
//
LOCUS       DAZKGF010000568          446 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZKGF010000568 DAZKGF010000000
VERSION     DAZKGF010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.571"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>446)
                     /locus_tag="ACHOQV_005443"
     CDS             complement(<1..>446)
                     /locus_tag="ACHOQV_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633634.1"
                     /translation="NPRFPTSAENQRDAIAKMLDLQYDRIYRLAKDIASKGLDPSENI
                     LVYPSEEESGFFVVAEGNRRVTALKLLHSPRLAPTEKARKAFEKLKITQLKDINVIDN
                     CVLFDDDEYEHWVNLKHTGQNAGVGRVEWTATEKARHLARMGKQSF"
BASE COUNT          129 a           98 c           81 g          138 t
ORIGIN      
        1 aaatgactgc ttacccatcc tagccagatg tcgtgctttt tctgttgcag tccattcaac
       61 tcttccaact ccagcatttt gaccagtatg ctttaaattg acccaatgct catattcatc
      121 atcgtcaaaa agaacgcagt tatctataac attaatatct ttaagctgag taatcttaag
      181 tttttcaaaa gctttccgtg ctttttctgt tggtgcaagt ctaggagagt gtaaaagttt
      241 aagggctgta actcgacgat taccttcagc aacgacgaaa aacccagatt cctcttcact
      301 aggataaact aatatgtttt cagacggatc taaacctttc gaagcaatat ctttagccaa
      361 gcgataaatg cggtcgtact gcaagtcaag catcttggca atagcatcac gctgattttc
      421 tgctgacgtc gggaaacgag ggttat
//
LOCUS       DAZKGF010000569          445 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZKGF010000569 DAZKGF010000000
VERSION     DAZKGF010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.572"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            23..445
                     /locus_tag="ACHOQV_005444"
                     /pseudo
     CDS             23..445
                     /locus_tag="ACHOQV_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150682.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          161 a           72 c           88 g          124 t
ORIGIN      
        1 taattttatc aaaggaggtt gtgtgacaat agtaagagat tctgttagtg gtgaaattgt
       61 tcatagtcat gatcttcggg aaatggatgt tctttatata caaaaaacca cttttgaatg
      121 tccgtatgac aaatgtagga tacctgctac tccatgtagc ttcaaagaaa taaatttaaa
      181 tcaatcatac tttcgatata aagagcctcg taggccggga tgtaagattc atgatcctaa
      241 gagtaaaata agtataactc aaagaataat aaaaagcata attcaccacc ggcactggct
      301 atatatcttc tgaaacttga tgtacctgaa ggaaattacg actcttctgg cagaagaatt
      361 aataaacagg cgcaagatga tgaaaaagaa ttggggggca aagtacaccc tgtgtcatct
      421 tcaagcatca aaccggtagt agact
//
LOCUS       DAZKGF010000570          444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZKGF010000570 DAZKGF010000000
VERSION     DAZKGF010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.573"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(99..308)
                     /gene="fumD"
                     /locus_tag="ACHOQV_005445"
     CDS             complement(99..308)
                     /gene="fumD"
                     /locus_tag="ACHOQV_005445"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFP9633635.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          131 a          105 c           69 g          139 t
ORIGIN      
        1 aattcgtata atttcagcag ttgttattta gaatgattaa ctggaccgca actgaagcaa
       61 gaatatcagg atgaagctta ccgcctcatc ctgaatggtc attttaccag tgcgttaatg
      121 acggtttcca tcgcctgctt ttccagctca ctgcgctgaa tgcgtttatt ggctaatgct
      181 gtacgtaata cgcctgcgat aacaatatgc tttggttcct gccccagatc gcgcatttcc
      241 agcacaactt taccgacaac tctgcacatt tctcggtaca gttcatcctc ttttgtccga
      301 tttcccattc tttataccta tttatcatgc caactatcag catatatcaa tctaaccaat
      361 taaacaaaaa tcaaacaaaa tcagacaaat aacgcgataa attattttaa ctgtagcaat
      421 tgagcgatga tgtatttata cacc
//
LOCUS       DAZKGF010000571          443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZKGF010000571 DAZKGF010000000
VERSION     DAZKGF010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.574"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>443
                     /locus_tag="ACHOQV_005446"
     CDS             <1..>443
                     /locus_tag="ACHOQV_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP9633636.1"
                     /translation="ATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVK
                     DDDGSRAGTVRDDAGSLGGYLNLVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWG
                     WLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFG"
BASE COUNT           87 a          123 c          143 g           90 t
ORIGIN      
        1 gccacgccgg aaagcagcgg cagctatggc ttcgtccgtc tggagggtga cctgctcaga
       61 acagaggttg ccggtatgtc tctgacgaca ggggtgtatg gtgctgcagg ccattcttcc
      121 gttgatgtta aggatgatga cggttcccgc gccggcacgg tccgggatga tgccggcagt
      181 ctgggcggat acctgaatct ggtacacaca tcctccggcc tgtgggctga cattgtggcc
      241 cagggaaccc gtcacagcat gaaagcgtca tcggacaata acgacttccg cgcccggggc
      301 tggggctggc tgggctcact ggaaaccggt ctgcccttca gtatcactga caatctgatg
      361 ctggagccac aactgcagta cacctggcag ggactctccc tggatgacgg ccaggataac
      421 gccggttatg tgaagttcgg gca
//
LOCUS       DAZKGF010000572          443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZKGF010000572 DAZKGF010000000
VERSION     DAZKGF010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.575"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>443
                     /locus_tag="ACHOQV_005447"
     CDS             <1..>443
                     /locus_tag="ACHOQV_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9633637.1"
                     /translation="LYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLSR
                     HSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGE
                     RGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWR"
BASE COUNT          107 a          110 c          139 g           87 t
ORIGIN      
        1 gttgtacgcc atagaggcgg atataagggg aatgccggca gagcagcggc ttgctgaacg
       61 tcagcgaaaa acgaaaccgc tgttgaaatc cctggaaagc tggttgcgtg aaaagatgaa
      121 gaccctgtcg cgacactcag agttggcgaa ggcgttcgcg tacgcactta accagtggcc
      181 ggcactgacg tactatgcga acgatggctg ggtggaaatc gacaacaaca tcgctgaaaa
      241 tgccctgcgg gcggtcagtc tgggtcgtaa aaacttcctg ttcttcggct ctgaccatgg
      301 tggtgagcgg ggagcgctac tgtacagcct gatcgggacg tgcaaactga atgacgtgga
      361 tccagaaagc taccttcgcc atgtgcttgg cgtcatagca gactggccgg tcaaccgggt
      421 cagcgaactg cttccgtggc gca
//
LOCUS       DAZKGF010000573          439 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZKGF010000573 DAZKGF010000000
VERSION     DAZKGF010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.576"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>439
                     /locus_tag="ACHOQV_005448"
     CDS             <1..>439
                     /locus_tag="ACHOQV_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP9633638.1"
                     /translation="TSAGQLQSQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSYSD
                     SGRLTGVHTTTSNLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRY
                     DRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSRHRLVHYTRTQ"
BASE COUNT          107 a          155 c          114 g           63 t
ORIGIN      
        1 atacctctgc cgggcagtta cagagccagc acctgaacag cctgcagtat gaccgcgatt
       61 acacctggaa cgacaacggc gaactcatcc gcatcagcag cccgcgacag acccggagtt
      121 acagctacag cgactccggc aggctgaccg gcgtccacac caccacctca aatctggata
      181 tccgcatccc gtatgccacg gacccggcag gtaaccgcct gcccgacccg gagctgcacc
      241 cggacagcac cctcagcatg tggccggata accgtatcgc ccgtgacgcg cactatcttt
      301 accggtatga ccgtcacggc aggctgacag agaaaaccga cctcatcccg gaaggggtta
      361 tccgcacgga tgatgagcgg actcaccggt accattacga cagccggcac cggctggtgc
      421 actacacgcg gacacaata
//
LOCUS       DAZKGF010000574          437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZKGF010000574 DAZKGF010000000
VERSION     DAZKGF010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.577"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            49..437
                     /locus_tag="ACHOQV_005449"
                     /pseudo
     CDS             49..437
                     /locus_tag="ACHOQV_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          126 a           85 c          108 g          118 t
ORIGIN      
        1 tacctggcgt cagtccttgc tgggagcggg ctttcgtaat ctttccctgg gcggtatatc
       61 atccaggact cgattgggct gaagggggga tggaatctgt accaatatcc gctgaatcca
      121 gtgcaacacg ttgatccatt agggcttagt actatgataa taggtaacgg ccccgttcct
      181 gataatcctt ttgggcatgc tgcggcggca aatcgatatg gattaatgag ttcgggaact
      241 ggggatgaga tgggagcctc tgtctctgat tattttaaaa agatgcaacc ccgcagagat
      301 acctggattt ggataataga tacaacagat gaagaagaac aatgtatgat gaataaggca
      361 atagacctta ataaaaaact aaaaactatt aacctcgggc ctcttcctgt tttaaataat
      421 tgttttagcc gaacgaa
//
LOCUS       DAZKGF010000575          436 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZKGF010000575 DAZKGF010000000
VERSION     DAZKGF010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.578"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(53..>436)
                     /locus_tag="ACHOQV_005450"
     CDS             complement(53..>436)
                     /locus_tag="ACHOQV_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633639.1"
                     /translation="IDITGIGKGVFDIVSRFAPREANAILYSVESKNRLVMKMIDVVA
                     HKRIEWAKDAIDEASRERTEIPASFMSIRRTTTKSGNALTFVAERSDVTGHADVFFAI
                     SHAVINEPVDYDYERPSTWAFGKAA"
BASE COUNT           87 a          126 c          108 g          115 t
ORIGIN      
        1 ggcgtgaatg cttttttcgt cacgcgtgac gttttgtttc tgcgtttctt gctcatgcgg
       61 ctttcccgaa ggcccatgtg gacgggcgtt catagtcata atctacaggc tcattaatca
      121 cggcgtgcga aatggcaaaa aacacatccg catgccccgt aacatccgac cgttccgcca
      181 cgaacgtcag cgcgttaccg ctttttgtgg tggtgcggcg tatggacatg aacgacgcgg
      241 ggatctccgt gcgttcacgg ctggcctcat caatggcgtc tttcgcccac tcgatacgct
      301 tatgggccac cacgtctatc attttcatca ccaggcggtt tttgctctcc acgctgtaaa
      361 gaatggcgtt agcctcacgt ggcgcaaagc gactgacgat atcaaacacc cctttcccga
      421 taccggtgat atctat
//
LOCUS       DAZKGF010000576          435 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZKGF010000576 DAZKGF010000000
VERSION     DAZKGF010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.579"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(223..>435)
                     /locus_tag="ACHOQV_005451"
     CDS             complement(223..>435)
                     /locus_tag="ACHOQV_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9633640.1"
                     /translation="AWLFGHTARPLTRTEMVVVFGVIFHEYGCTFLADQLGISMKTVC
                     AHLYNAMEKSGLRGVSVKYLCSTADR"
BASE COUNT          127 a          120 c           74 g          114 t
ORIGIN      
        1 catcacttat attattctta tacagaaaac cgacaatttc agatatccgg gaagaagata
       61 ttatcaccac attatctgtc tgcatgccgc tgataatgaa tttaccggga ttaatatact
      121 ccttcactcc ctccagaaaa taactgttat cacaaataaa atgcataccc gttccacatg
      181 aaatttatca cgcctggcca cataacatta attatcccgg atttaccggt ctgcggtgct
      241 gcagagatat ttaacactga cacctcgcag accgcttttc tccatcgcat tgtaaagatg
      301 cgcacatacc gtcttcatgc ttatccccag ctggtctgcc agaaaggtac agccgtactc
      361 atggaaaata accccgaaga ccaccaccat ctcggtacgg gtcagtggtc ttgccgtatg
      421 tccgaacagc cacgc
//
LOCUS       DAZKGF010000577          435 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZKGF010000577 DAZKGF010000000
VERSION     DAZKGF010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.580"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..213
                     /locus_tag="ACHOQV_005452"
     CDS             <1..213
                     /locus_tag="ACHOQV_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9633641.1"
                     /translation="AWLFGHTARPLTRTEMVVVFGVVFHEYGFTFLADQLGISMKTVC
                     AHLYNAMEKNGLRGVSIKYLCSTADR"
BASE COUNT          117 a           74 c          115 g          129 t
ORIGIN      
        1 gcgtggctgt tcggacatac ggcaagacca ctgacccgta ccgagatggt ggtggtcttc
       61 ggagttgttt tccatgagta cggttttacc tttctggcag accagctggg gataagcatg
      121 aagacggtat gtgcgcatct ttacaatgcg atggagaaaa atggtctgcg cggcgtcagt
      181 attaaatatc tctgcagcac cgcagaccgg taaatccggg ataattaatg ttatgtggcc
      241 aggcgtgata aatttcatgt ggaacgggta tgcattttat ttgtgataac agttattttc
      301 tggagggaat gaaggagtat attaatcccg gtaaattcat tatcagcggc atgcagacag
      361 ataatgtggt gataatatct tcttcccgga tatctgaaat tgtcggtttt ctgtataaga
      421 ataatataag tgata
//
LOCUS       DAZKGF010000578          435 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZKGF010000578 DAZKGF010000000
VERSION     DAZKGF010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.581"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>435
                     /locus_tag="ACHOQV_005453"
     CDS             <1..>435
                     /locus_tag="ACHOQV_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9633642.1"
                     /translation="NETAKQFNTSIIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYL
                     SESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGR
                     YFNAGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQD"
BASE COUNT          134 a           81 c           83 g          137 t
ORIGIN      
        1 ttaatgaaac ggcaaaacaa tttaatacct caattattgt atatttaatt gaccccaaat
       61 actttgctga tctgccgacg tcacagtttt ggtcgtacgc aacatacttc agggtattgt
      121 cttttgaata tctgagtgaa agtatttcta cactgctgta tctggatgcc gatgttgttt
      181 gtaaaggaag cctgaaacct ctcacagaaa ttatatttaa agatgagttt gctgcggtca
      241 ttcctgacaa tgatagtact caggcggcat gtgcaaaacg cctcaacatt cccgaaatga
      301 atggacgtta tttcaatgca ggcgttatct atgtcaatct taaaaaatgg catgaagcaa
      361 atttgacacc gtatttactc acgcttttac gaggggaaac taaatatggc tctcttaaat
      421 atttagatca ggatg
//
LOCUS       DAZKGF010000579          431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZKGF010000579 DAZKGF010000000
VERSION     DAZKGF010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.582"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>431
                     /locus_tag="ACHOQV_005454"
     CDS             <1..>431
                     /locus_tag="ACHOQV_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP9633643.1"
                     /translation="HPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFE
                     DAPEIIQSGLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAHM
                     PFIGSVGPAFFRKDTMEEVAAIKDIGNHFERAEYIKWNAFR"
BASE COUNT           93 a           91 c          132 g          115 t
ORIGIN      
        1 catccggaat ttcagaaagt ggagtccctg tggcggggcg tgaagtctct ggtggataaa
       61 acggacttcc gccgtaatgt gaaaattgaa ctgctggatc tgtccaaaga cgatctgcgt
      121 caggattttg aggatgcgcc ggaaattatt cagagtggtc tttatctgca gacttatgtg
      181 gcggagtacg atacgccggg tggtgaaccc attgcagccc tgatttctgc ctgggagttt
      241 gatgcgtctg cgcaggatgt ggcgctgctg aaaaatattt cccgggtggc tgcgtccgcg
      301 catatgccat ttattggttc agttggcccg gctttcttcc ggaaggatac gatggaagag
      361 gtggccgcca ttaaggatat tggcaatcac tttgagcgtg ccgagtacat caagtggaat
      421 gctttccgtg a
//
LOCUS       DAZKGF010000580          427 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZKGF010000580 DAZKGF010000000
VERSION     DAZKGF010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.583"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>427)
                     /locus_tag="ACHOQV_005455"
     CDS             complement(<1..>427)
                     /locus_tag="ACHOQV_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633644.1"
                     /translation="NKMADFFSSTASGVKHKPLKHVKIDNRLFPSPEYIIFSDYLTKN
                     SGPFVNHYFCSIPYSKEEECRLGVTIMKYASHRNKPLSLWCLGMAEGAMARTISQLSK
                     GQVLSLTTSPTKENEPVFFSHGSHIHSYFICSPFFLVHEE"
BASE COUNT          131 a           71 c           84 g          141 t
ORIGIN      
        1 tttcttcatg aacaagaaag aatggggagc aaataaagta agagtgaata tgagaaccat
       61 gactaaagaa tacaggttca ttttcttttg ttgggctggt tgttaatgat aaaacctgtc
      121 cttttgatag ttgagaaatt gttctggcca tggctccttc tgccattcca agacaccata
      181 agctcaatgg cttatttcga tggcttgcat atttcataat agtcacaccc agcctgcatt
      241 cttcttcttt gctgtatgga atactgcaaa agtagtggtt aacaaatgga ccactgtttt
      301 tcgtaagata gtcagaaaaa attatatact caggagatgg gaaaagcctg ttatcaattt
      361 ttacatgctt caaaggttta tgttttactc cagatgctgt tgatgagaaa aaatcagcca
      421 ttttgtt
//
LOCUS       DAZKGF010000581          425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZKGF010000581 DAZKGF010000000
VERSION     DAZKGF010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.584"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            99..>425
                     /locus_tag="ACHOQV_005456"
     CDS             99..>425
                     /locus_tag="ACHOQV_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9633645.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRY"
BASE COUNT          103 a           88 c           87 g          147 t
ORIGIN      
        1 atcaaaacct ttataacatc cgaaaaagaa agatacgaat agcatttaca caatacccgg
       61 aatagacttt cgtcactttt aattaaaggg atgtttttat gcagtggtat ttagccgtat
      121 taaaaaatta tgttggtttc tctggtcgcg cacgtcgtaa agagtactgg atgtttactc
      181 tgattaacgc catcgtcggc gctattatca atgtcattca attgatttta ggtttagagt
      241 ttccattcct ttctctcatt tacctggcag caacaatcat tccggtcatt gctctgtgcg
      301 ttcgtcgtct gcacgatact gaccgttcag gtgcgtgggc gcttctctat ctggttccga
      361 tcattggctg gttagttctg tttgtatttg cctgtttaga aggcaactct ggcagcaatc
      421 gttac
//
LOCUS       DAZKGF010000582          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZKGF010000582 DAZKGF010000000
VERSION     DAZKGF010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.585"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..135
                     /locus_tag="ACHOQV_005457"
     CDS             <1..135
                     /locus_tag="ACHOQV_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9633646.1"
                     /translation="MATDDESALLPLWKKYRVLLNRTDTGNAPDIVWPESPSPDHHAG
                     "
     gene            147..>421
                     /locus_tag="ACHOQV_005458"
     CDS             147..>421
                     /locus_tag="ACHOQV_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633647.1"
                     /translation="MTWTRKTISVSADMPPLSCSVIPVTPWTYGLGRYEESGVYLSPP
                     NAINWLAGKMAGSRASGDVTIIMIAENTHDLFIQSMAALTAVLPVPV"
BASE COUNT          102 a          125 c          114 g           80 t
ORIGIN      
        1 atggcgaccg acgatgaaag cgccctgctt cccctgtgga aaaaataccg ggtattactg
       61 aaccggacag acacgggcaa cgcgccggat attgtctggc ctgaatcccc ttcgccggat
      121 catcacgcag gctgaccgga ggcaccatga cgtggacaag aaaaacaatc agcgtcagcg
      181 ctgacatgcc cccgctgagc tgttccgtca tccccgtcac accgtggact tacgggctgg
      241 gacgatatga agaatcgggg gtatatttaa gccctccgaa cgccataaac tggctggccg
      301 ggaaaatggc cggaagccgt gcatccggcg acgtgaccat tatcatgatt gccgaaaaca
      361 cccacgatct ttttatacag tcaatggcgg cactgaccgc cgttctgccc gtgccggtat
      421 t
//
LOCUS       DAZKGF010000583          420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZKGF010000583 DAZKGF010000000
VERSION     DAZKGF010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.586"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>420
                     /locus_tag="ACHOQV_005459"
     CDS             <1..>420
                     /locus_tag="ACHOQV_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9633648.1"
                     /translation="NGGQTPYDDKPLSVTGIDALGLSINKITTNNMEFNGDVAIVSED
                     SPYLHNGIVFLNGIPFKIIGVIPKMKMDFLDSLGLSKYQSNNQLFVPLSTLFRFKLSS
                     NIDNVKIVLSDKVGENDINTTKKTLGEEKIPEYNIITA"
BASE COUNT          155 a           67 c           78 g          120 t
ORIGIN      
        1 caatggcggt caaacacctt acgatgacaa acctttatca gtgacaggta ttgatgcgct
       61 aggtttatcc ataaataaaa taaccaccaa taacatggaa tttaatggag atgttgctat
      121 tgttagtgag gatagtccat atttacacaa tgggattgtt ttccttaatg ggataccgtt
      181 taaaattatt ggcgtaattc caaaaatgaa aatggacttc ctggacagtc ttgggttatc
      241 gaaatatcag agcaacaatc agctctttgt ccccttaagt acattattca gattcaagct
      301 aagcagtaat attgacaatg ttaagatagt gttaagcgat aaagttggcg aaaatgatat
      361 taacacaaca aaaaaaacgc ttggtgagga aaaaatacca gaatacaata ttataacagc
//
LOCUS       DAZKGF010000584          419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZKGF010000584 DAZKGF010000000
VERSION     DAZKGF010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.587"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            10..99
                     /locus_tag="ACHOQV_005460"
     CDS             10..99
                     /locus_tag="ACHOQV_005460"
                     /inference="COORDINATES: protein motif:HMM:NF022416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HFP9633649.1"
                     /translation="MARYPGCEMQIVEKINEVYSPKLEIELKN"
     gene            150..287
                     /locus_tag="ACHOQV_005461"
     CDS             150..287
                     /locus_tag="ACHOQV_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633650.1"
                     /translation="MRRHYLPGEPDDEESIARAIWLDEYFARTRANKTAEGIAIAFNG
                     R"
BASE COUNT           94 a          116 c          124 g           85 t
ORIGIN      
        1 gctgacctga ttgcgcgtta tccgggctgt gagatgcaga tcgtcgagaa gattaacgag
       61 gtttactccc cgaaactgga gattgagtta aaaaactgac cgcgcgggtc gaaagcatcc
      121 gccgcaatcc ccttgagcag tatctggcat tgcggcgtca ctacctcccc ggagagccgg
      181 acgacgaaga gagcatcgcc cgtgccatct ggctggatga atattttgcc cgtacccgcg
      241 ccaacaagac cgctgaaggt atagcgatcg catttaacgg aaggtgacat ggccagccag
      301 ctggatttca ttatcaatct gacagaccag cttacccgcc cgctgcggca ggttcaggaa
      361 tccgtgacgg gattcgccga acgctcgcag ggcgcgttcg cgcgtatcgg tgtgggtgt
//
LOCUS       DAZKGF010000585          415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZKGF010000585 DAZKGF010000000
VERSION     DAZKGF010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.588"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          113 a           66 c          106 g          130 t
ORIGIN      
        1 gtctcctggc acagaaaatg gtgatttttg atttgaatca ttgaaatcag ttctgctaat
       61 aagaagacat gctgaaaagc cgattttcag ggggtgattg aagggattgt gagtttttcc
      121 ttcggctttt aacgtaactt cctgttttcc tgatattcca cggcaagatt aatttttagc
      181 ggagcagaat gaataaaatc ttcatctggt gtttttgttg cgcgtaaatg caggtgtaac
      241 tgtggatgtt atccactgaa gagctgctga tgaggatgaa gacatgctga ctaaaaaaat
      301 gttactcgtt tgtgcttcga gtgttgcgct gagtgcctgt tctgcacagg aaattgcaga
      361 gctgaacagg cagataagtg acggtgcatt tgctgtgaca aaagtactga acgta
//
LOCUS       DAZKGF010000586          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZKGF010000586 DAZKGF010000000
VERSION     DAZKGF010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.589"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..282
                     /locus_tag="ACHOQV_005462"
     CDS             <1..282
                     /locus_tag="ACHOQV_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HFP9633651.1"
                     /translation="LTLFKNAAFVVTTSFHGTVFSIINQVPFFTLEFGNEIDLRSRSL
                     LEMLNLNERMISDDANLNYEKLFLEFDEAHSILESKRQDSLSFIERALS"
BASE COUNT          141 a           57 c           76 g          137 t
ORIGIN      
        1 ttaactttat ttaaaaatgc agctttcgtg gtgaccactt cttttcacgg aactgtattc
       61 tcaattataa atcaggtgcc attctttact ttggaatttg gtaacgagat agacttaaga
      121 agccgttcac ttcttgaaat gcttaatttg aatgaacgaa tgatcagtga cgatgcaaat
      181 ttgaattatg agaagctttt cttggaattt gacgaggctc attcaatatt agaaagtaaa
      241 aggcaggatt ctttaagttt cattgagaga gctctgagtt aaataaaatg actaaagttt
      301 taatagtatt ccatgacggt gataaaaaaa gtggtgcaac agcctcaatg ttagatcttt
      361 taacagagct gatcaaatgt aaagatttga aaataatatg cttaattcca a
//
LOCUS       DAZKGF010000587          407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZKGF010000587 DAZKGF010000000
VERSION     DAZKGF010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.590"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>407
                     /locus_tag="ACHOQV_005463"
     CDS             <1..>407
                     /locus_tag="ACHOQV_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP9633652.1"
                     /translation="PDRPYIAYALHDSEHPDHVTSDNHTRNVWRTPANNKLRMEDKRQ
                     EEHIKLATEYGKTQLNMGHLVNSRREKRGAGFELRTDEHGAVRAAKGLFLTADAQARA
                     QGPVLEMAAAISQINQANSQMQALNSAAEAAGAL"
BASE COUNT          106 a          112 c          127 g           62 t
ORIGIN      
        1 ccggatcgtc cctacatcgc ttatgccctg catgattcag agcatccgga tcatgtcacc
       61 agtgacaatc atacgcggaa cgtgtggcgc acgccggcga acaataagct gcggatggag
      121 gataagcgcc aggaagagca tatcaagctc gcaacggaat atggcaaaac acagctgaac
      181 atgggacacc tggtcaacag ccggcgtgaa aagcgaggtg ccggttttga actgcgaacg
      241 gatgagcatg gggctgtacg tgcagcaaaa gggctgttcc tgacggcaga tgcccaggcc
      301 agagcccagg ggccggtgct ggaaatggcg gccgcgataa gtcagattaa ccaggctaac
      361 agccagatgc aggccctgaa cagcgctgcc gaagccgccg gcgccct
//
LOCUS       DAZKGF010000588          404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZKGF010000588 DAZKGF010000000
VERSION     DAZKGF010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.591"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..390
                     /locus_tag="ACHOQV_005464"
     CDS             <1..390
                     /locus_tag="ACHOQV_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9633653.1"
                     /translation="KMAGIIGNVFGDLWNVIKNSFNRTWNWLAEKLNKIPGISIDMKP
                     TEQVNTMTRGATGSTVPPQIAETAVPALAGNRISAELPPGGIRNQIRTDTSTRIDNSR
                     TFGNLTINTQNPMTPGQLDEWTELYAG"
BASE COUNT          114 a          119 c          112 g           59 t
ORIGIN      
        1 aaaatggcgg ggatcatcgg taacgtcttc ggggacttat ggaacgtcat taaaaactcg
       61 tttaaccgga cgtggaactg gcttgcggaa aagctgaaca aaatccccgg tatcagtatc
      121 gacatgaagc ccacggagca ggtgaacacc atgacgcggg gcgcaacagg cagcaccgta
      181 cctccgcaga ttgcagaaac cgccgtcccg gcactggcag gcaaccgcat cagcgcagag
      241 ctgccacccg gaggcatacg caaccagata cgcacggaca cctcaacccg tatcgacaac
      301 agccggacgt tcggcaatct gacaatcaac acgcagaacc cgatgacgcc ggggcaactg
      361 gatgaatgga cggagcttta cgcgggatga acacgccatc gctt
//
LOCUS       DAZKGF010000589          404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZKGF010000589 DAZKGF010000000
VERSION     DAZKGF010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.592"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>404
                     /locus_tag="ACHOQV_005465"
     CDS             <1..>404
                     /locus_tag="ACHOQV_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP9633654.1"
                     /translation="FGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAP
                     FVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQI
                     GTFAINFSRPAGQVIAQYYEFLRLGREGYTKV"
BASE COUNT           76 a          121 c          115 g           92 t
ORIGIN      
        1 ttcggcgtga cctacaccgg taactatgag ttcccgcagc cgctgcacga tgcgctggat
       61 aaattccagg ccgacaccgg tatcgacatc gacatgcaca tcgacgccgc cagcggtggc
      121 ttcctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
      181 tcgatcagtg cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc
      241 tggcgtgatg aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctcggcggt
      301 cagattggta ctttcgccat caacttctcc cgcccggcgg gtcaggtgat tgcacagtac
      361 tatgaattcc tgcgcctcgg tcgtgaaggc tataccaaag taca
//
LOCUS       DAZKGF010000590          402 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZKGF010000590 DAZKGF010000000
VERSION     DAZKGF010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.593"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>402)
                     /locus_tag="ACHOQV_005466"
     CDS             complement(<1..>402)
                     /locus_tag="ACHOQV_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633655.1"
                     /translation="AGIRPELLGGVAWIESGGMPENYKFQIYETKRMIGSLDMPENKT
                     SFGSMGIQIRTAAITLGLDPSELTTRNQLELATCLMEDDFTFKIAATHLRDLALFDYP
                     SSATLYMTNEQYIMAGIRYNRGVERDLGFFI"
BASE COUNT          124 a           90 c           69 g          119 t
ORIGIN      
        1 aaataaaaaa acccaaatct cgttcaacac cacgattata tctaatacca gccataatat
       61 actgctcatt cgtcatatac agtgttgccg aagagggata atcaaaaagg gcaagatctc
      121 ttaaatgagt agcagcaatt ttaaaggtaa aatcatcttc catcaggcat gtcgctaact
      181 ctaactgatt tctggtagtt aattctgaag ggtcaaggcc tagagtaatc gctgcggttc
      241 gtatttgtat acccattgag ccaaatgatg ttttattttc aggcatatct aacgatccga
      301 tcattctttt tgtttcatat atctgaaact tataattttc aggcatacct cctgactcaa
      361 tccaggccac gccgcccagt aactctggac gaatccctgc gg
//
LOCUS       DAZKGF010000591          400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZKGF010000591 DAZKGF010000000
VERSION     DAZKGF010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.594"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..361
                     /locus_tag="ACHOQV_005467"
     CDS             <1..361
                     /locus_tag="ACHOQV_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9633656.1"
                     /translation="RPLIENGSIEPRYVDAIYHSHDTIGPYYVVGPGIAMPHARPEEG
                     ANKLSLALTLIPSGVNFDADENDPVKLLIVLAATDSTSHIEAISQLAKLFDNEKDIQA
                     ILTAKTTQDILSVIARY"
BASE COUNT          105 a           95 c           96 g          104 t
ORIGIN      
        1 ccgaccgttg attgaaaacg gctctatcga accgcgttat gttgatgcga tctatcactc
       61 gcacgacact atcggtccgt actatgtggt tggccctggt attgcgatgc ctcatgcgcg
      121 cccggaagag ggggctaaca agctgtcatt agcactgacc ctgatccctt caggtgtgaa
      181 ttttgatgcc gatgaaaacg atccagtgaa attactgatt gtgctggctg caaccgacag
      241 taccagccac attgaagcga tttctcaact cgccaagtta ttcgataacg aaaaagacat
      301 acaggccatt ttgacggcca aaacgacgca ggacattttg tctgttattg cgcgctatta
      361 atccagagga acatttatga aaattacagt ggtttgcggt
//
LOCUS       DAZKGF010000592          400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZKGF010000592 DAZKGF010000000
VERSION     DAZKGF010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.595"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..167)
                     /locus_tag="ACHOQV_005468"
     CDS             complement(<1..167)
                     /locus_tag="ACHOQV_005468"
                     /inference="COORDINATES: protein motif:HMM:NF024418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFP9633657.1"
                     /translation="MNKIYALKYSSLTGGLIAVSELSKKVTGKTDRRLMTVSLVLSVT
                     LSALPGKASTV"
BASE COUNT          121 a           75 c           68 g          136 t
ORIGIN      
        1 ctgaccgttg atgctttacc cggtaaagca gaaagagtca ctgataatac cagggaaacc
       61 gtcattaatc ttctatcggt ttttcctgtg accttcttac ttaattctga cacagctata
      121 agcccaccag taagggagct atatttaagc gcgtatattt tattcatata attcctgtta
      181 tataattata tggtattgaa atgtattatc tgtccggaaa gagtgagtgc tgggcactca
      241 ctctgaatct attttaaatg gcatcagaga tacctgtcca cacttaagat ttttcctgcg
      301 aaaaacaaac attaatatat tttatgtatt tcgcatggtg atgatttata cacctgcact
      361 gaatttttct gtatttccag tactgaatcg ataaacagaa
//
LOCUS       DAZKGF010000593          398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZKGF010000593 DAZKGF010000000
VERSION     DAZKGF010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.596"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>398
                     /locus_tag="ACHOQV_005469"
     CDS             <1..>398
                     /locus_tag="ACHOQV_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP9633658.1"
                     /translation="TMTWSRYGQLLTVTDCSGYVTRYDHDRFGQMTAAHREEGLSQYR
                     AYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDTWGKAIRTTQGGL
                     TRSMEYDAAGRVIRLTSENGSHTTFRYDVL"
BASE COUNT           96 a          111 c          120 g           71 t
ORIGIN      
        1 accatgacat ggagccgtta cggtcagttg ctgaccgtca ctgactgttc cggttatgta
       61 acccgttatg accatgaccg ttttggtcag atgacggcgg cgcaccgcga ggaagggctg
      121 agtcagtatc gcgcatacga cagccgtgga cagttaattg cggtgaaaga cacgcagggc
      181 catgaaacgc ggtatgaata caacgccgcc ggtgacctga ccgccgtcat tgccccggac
      241 ggcagcagaa acgggacaca gtacgatacg tggggaaagg ccatccgtac cacgcagggt
      301 ggtctgacgc gcagtatgga atacgatgct gccggacggg tcatccgcct gaccagtgaa
      361 aacggcagcc acaccacctt ccgttacgat gtactcga
//
LOCUS       DAZKGF010000594          395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZKGF010000594 DAZKGF010000000
VERSION     DAZKGF010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.597"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..255
                     /locus_tag="ACHOQV_005470"
     CDS             <1..255
                     /locus_tag="ACHOQV_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633659.1"
                     /translation="HELANILTREKILTTREKKGNLISLFDGFTLFHCVGMITFLIHY
                     LRTPEEKVENIFMLYGADKNNKLRRRLIYDALGIIQSQQE"
BASE COUNT          147 a           66 c           65 g          117 t
ORIGIN      
        1 catgaactgg caaatattct tacccgcgaa aaaatactta caacacggga aaaaaaagga
       61 aacctgataa gcctgtttga tggattcact ttattccatt gtgttggaat gataaccttt
      121 cttatccatt atctcagaac acctgaagaa aaagttgaaa atatatttat gttatatggt
      181 gcagataaaa acaataaact acgcagaaga ctgatttatg acgcactagg aataattcag
      241 tctcagcagg agtgaagagt taaacaggca aattattttt tataaaaagg ggattgttaa
      301 gtaatcccct gttaatcaaa atacgctttc ctgacgatct aataattatc agctcaatac
      361 atttgacata aaagcggttt cttaattact actgc
//
LOCUS       DAZKGF010000595          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZKGF010000595 DAZKGF010000000
VERSION     DAZKGF010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.598"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>386
                     /locus_tag="ACHOQV_005471"
     CDS             <1..>386
                     /locus_tag="ACHOQV_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9633660.1"
                     /translation="ALVVVAIKFWQPVKAFIRGFIDGFTGALDAMSPVTAAFGEIARA
                     LAPVWQGIKTLFGAFTDLFTPVQLTTGQMNSVTRAGETCGRVVAAAVSLISPALEVVI
                     SVIRTLVDIFAIVIRGWQDVVAAFDPT"
BASE COUNT           69 a          109 c          122 g           86 t
ORIGIN      
        1 gctctggtgg tggtggcaat taagttctgg cagcccgtca aagcctttat tcgcggcttt
       61 attgatggtt ttaccggcgc actggacgcc atgagcccgg tgactgccgc gttcggtgaa
      121 attgcccgtg cgctggcccc ggtatggcag ggcataaaaa ccctgttcgg cgccttcact
      181 gatctgttca caccggttca gctgaccacg ggacagatga acagcgtcac ccgtgccggg
      241 gaaacatgcg gtcgcgtggt ggccgcagcg gtaagcctga tatcccccgc gctggaggtg
      301 gttatcagcg tgatcagaac cctggtcgac atcttcgcca tcgtcatcag agggtggcag
      361 gacgtggtgg ctgcatttga cccgac
//
LOCUS       DAZKGF010000596          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZKGF010000596 DAZKGF010000000
VERSION     DAZKGF010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.599"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>386
                     /locus_tag="ACHOQV_005472"
     CDS             <1..>386
                     /locus_tag="ACHOQV_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9633661.1"
                     /translation="ALVVVAIKFWQPVKAFIRGFIDGFTSALDAMSPVTAAFGDIARA
                     LAPVWQGIKTLFSAFSDLFAPVQLTTGQMNSVTRAGETCGRVVAAAVSLISPALEVVI
                     SVIRTLIDIFAIVIRGWQDVVAAFDPT"
BASE COUNT           69 a          110 c          121 g           86 t
ORIGIN      
        1 gctctggtgg tggtggccat taagttctgg cagcccgtca aagcctttat tcgcggcttt
       61 attgatggtt ttaccagcgc actggacgcc atgagcccgg tgactgccgc gttcggtgac
      121 attgcccggg cgctggcccc ggtatggcag ggcataaaaa ccctgttcag tgccttctcc
      181 gatctgtttg caccggtaca gctgaccacg ggacagatga acagcgtcac ccgtgccggg
      241 gaaacatgcg gtcgcgtggt ggccgcagcg gtaagcctga tatcccccgc gctggaggtg
      301 gttatcagcg tgatcagaac cctgatcgac atcttcgcca tcgtcatcag agggtggcag
      361 gacgtggtgg ctgcatttga cccgac
//
LOCUS       DAZKGF010000597          380 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZKGF010000597 DAZKGF010000000
VERSION     DAZKGF010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.600"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>380
                     /locus_tag="ACHOQV_005473"
     CDS             <1..>380
                     /locus_tag="ACHOQV_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633662.1"
                     /translation="LMHYSVFTDKTHQIIKQKYFNDIVPMILALDIRNTYRPDNEMAF
                     YYHIHSFLTQIPDNEDDIYHAARTYLRNYVKLCLSGYTPANAHFKDIFDGVYEFIRNI
                     RKNSTPGKTKLIATINTCKETCKHL"
BASE COUNT          141 a           77 c           54 g          108 t
ORIGIN      
        1 ctaatgcact attctgtttt tacagacaaa acacatcaaa taataaaaca aaaatatttt
       61 aatgacattg taccaatgat tctcgcactc gacatcagga acacatatcg cccggataat
      121 gagatggcat tttactatca tattcattct tttctcactc agataccgga taatgaggat
      181 gatatatatc atgctgcaag gacatatctg cgaaattacg ttaagttatg tttatccgga
      241 tacacaccag cgaatgcgca tttcaaagat atctttgatg gcgtatatga attcattcgt
      301 aatattcgca aaaacagtac accaggaaaa acaaaactta tcgcaactat caacacatgc
      361 aaagaaacct gtaaacatct
//
LOCUS       DAZKGF010000598          379 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZKGF010000598 DAZKGF010000000
VERSION     DAZKGF010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.601"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(79..>379)
                     /locus_tag="ACHOQV_005474"
     CDS             complement(79..>379)
                     /locus_tag="ACHOQV_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9633663.1"
                     /translation="PLRDSAKHSVRELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGM
                     TFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT           76 a          113 c          114 g           76 t
ORIGIN      
        1 cccggcatat ctgttgctcc ctgaaagtaa aaagccccgg tcatgatgac cggggcctga
       61 aggggagtga cctgattatc agaaagtcac attcagcgtg gcctgaccgt tatagccttc
      121 agcgctgctg ccgctgacgc tgtgggcata accggcctga acgcccaggg tgatattttc
      181 ccggacacgg gcttccagtc cggcctgcag gtccagtgac gtgccattct gggacggtga
      241 gaacgtcatc ccgctgcctg cggtggaggt ccccacacgc atatcacccc gggagctgaa
      301 ggtgcggata acggaaggct gtacccacca gttcaccggc agttcacgca cactgtgttt
      361 tgcactgtca cgcaggggg
//
LOCUS       DAZKGF010000599          379 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZKGF010000599 DAZKGF010000000
VERSION     DAZKGF010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.602"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>379
                     /locus_tag="ACHOQV_005475"
     CDS             <1..>379
                     /locus_tag="ACHOQV_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9633664.1"
                     /translation="PANALTGKPYTGVNVLLLWLAADEAGYVSNDWLTYRQAQQLGGH
                     VRKGEVATLAVIYKDWKKQAVDEHGNRLTDSEGVPLMETVPVLKSNPLFNVGQCDGLP
                     EHLMVKAEGSADALPADTFSERVL"
BASE COUNT           88 a           88 c          130 g           73 t
ORIGIN      
        1 ccggcaaatg ccctgaccgg gaagccttat acaggggtga acgtcctgtt gctgtggctg
       61 gccgcggatg aagccgggta tgtcagcaat gactggctga cgtaccgtca ggcgcaacaa
      121 ctgggtggcc acgtccgtaa aggggaggtg gccacgctgg cggtgattta caaagactgg
      181 aaaaaacagg ctgtggatga acacggtaac cggctgacgg acagtgaggg ggtgcctctc
      241 atggaaaccg taccggtact gaaatcgaat cctctgttta atgtggggca gtgtgacggt
      301 ttacctgaac atctgatggt gaaggcggaa ggcagcgcgg atgcactacc ggcagatacc
      361 ttcagtgagc gggtgctgc
//
LOCUS       DAZKGF010000600          378 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZKGF010000600 DAZKGF010000000
VERSION     DAZKGF010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.603"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>378
                     /locus_tag="ACHOQV_005476"
     CDS             <1..>378
                     /locus_tag="ACHOQV_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP9633665.1"
                     /translation="GWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIR
                     YRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEGGLKRVVKKEHA
                     DGSVTQSQFDAVGRLKAQTDAAGR"
BASE COUNT           93 a          103 c          131 g           51 t
ORIGIN      
        1 ggctggacgc cgcgcggcga actggcggcg gtgtatgacc gcagcaatac acaggtgcgc
       61 agctttactt acgatgataa ataccggggc cggatggtgg cgcaccgtca cacgggccgg
      121 ccggaaatcc gttaccgtta cgacagtgac gggcgggtga cagaacagct aaacccggca
      181 ggcttaagct acacgtatca gtatgagaaa gaccacatca ccatcaccga cagcctggac
      241 cgccgtgaag tgctgcacac gcagggcgaa ggcgggctga agcgggtggt gaaaaaggaa
      301 cacgcggacg gcagcgtcac gcagagtcag tttgacgcgg tgggcaggct taaggcacag
      361 acggatgccg caggccgg
//
LOCUS       DAZKGF010000601          377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZKGF010000601 DAZKGF010000000
VERSION     DAZKGF010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.604"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>377
                     /locus_tag="ACHOQV_005477"
     CDS             <1..>377
                     /locus_tag="ACHOQV_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HFP9633666.1"
                     /translation="VKYIIQTYYKNESTNLNLNIPKSTKYRLVKRMLSTLGFEQLTPS
                     IKNELSERLNDVATICMEPKYIFDELLAFLGQRRIALPGYSTVQNLVSDALNFERKRT
                     INILSKRIYPATAQKITSIIHDD"
BASE COUNT          134 a           68 c           58 g          117 t
ORIGIN      
        1 tgtgaaatat attatccaga catattataa aaacgaaagt actaatctca atttaaacat
       61 acctaaaagt acaaagtacc gccttgttaa acgaatgctt tctactctag gatttgaaca
      121 actcacccca tctataaaaa atgagttaag tgaacgtctg aatgatgttg ctactatatg
      181 tatggaacca aaatatattt ttgatgaatt acttgctttt ttaggacagc ggcgtatagc
      241 tcttcctgga tacagtactg tccagaacct cgtttcagat gctttaaact ttgaaaggaa
      301 aaggacaatt aatattttgt caaagagaat ttatcctgcc actgcacaaa agattacatc
      361 catcatccat gatgatg
//
LOCUS       DAZKGF010000602          375 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZKGF010000602 DAZKGF010000000
VERSION     DAZKGF010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.605"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..190
                     /locus_tag="ACHOQV_005478"
     CDS             <1..190
                     /locus_tag="ACHOQV_005478"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633667.1"
                     /translation="FIEKTGVATPMTGTFESLTGLGSDKASPAPEYEKSDIEQLPPSV
                     PENNIPEVCEESVLISEK"
BASE COUNT          120 a           84 c           68 g          103 t
ORIGIN      
        1 ttttattgaa aaaacaggtg tagcaacacc gatgactggt acgtttgaaa gcttaacagg
       61 ccttggaagt gataaagcct cccctgctcc tgagtatgaa aagtctgata ttgaacaatt
      121 accaccatcg gtgccagaaa acaatattcc agaggtgtgt gaagagtctg tgcttatatc
      181 agaaaaatga atatttgaag aaccattacc aacagttgct acaacgtgtc ctgtattagt
      241 tgtaaacttc ataccaataa catcaactcc agatatatta ttgccaatgg caaaacacac
      301 cccacctccc cccttgcagt aggttatctc accgctaaat tttgcatgac acaatctctc
      361 gatagtggcg ttgtt
//
LOCUS       DAZKGF010000603          373 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZKGF010000603 DAZKGF010000000
VERSION     DAZKGF010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.606"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..315
                     /locus_tag="ACHOQV_005479"
     CDS             <1..315
                     /locus_tag="ACHOQV_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9633668.1"
                     /translation="KTTSSFDYGTKTTSTNGQHEHSVPLRRQDGGGINFDWLDGSSSG
                     SHIGNGYVPAAGDHQHTVPIGAHTHTLALGSHGHGLTINPTGNAENTVKNIAFNYIVR
                     LA"
BASE COUNT          117 a          110 c           73 g           73 t
ORIGIN      
        1 aaaacgacct catcatttga ctacggaacc aaaacaacct ctacaaacgg acaacatgaa
       61 cacagcgtcc ccctgagaag acaagatggc ggaggcataa actttgactg gctggatggt
      121 tcatcgtccg gttctcatat tggtaatgga tacgttcctg ccgcaggtga ccaccaacat
      181 accgtaccca ttggcgcaca cacccacacg ctcgcactgg gttcacatgg tcacggcctg
      241 accattaacc ccaccggtaa tgccgaaaac accgtcaaaa acatcgcatt taactacatc
      301 gtgaggcttg cataatgacc tttaaaatga gcgaacaccc acagacaatt aaaatctaca
      361 acctgtcagc cgg
//
LOCUS       DAZKGF010000604          367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZKGF010000604 DAZKGF010000000
VERSION     DAZKGF010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.608"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..117
                     /locus_tag="ACHOQV_005480"
     CDS             <1..117
                     /locus_tag="ACHOQV_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFP9633669.1"
                     /translation="VDNSIQPEESVSFSYESIIWEHCSAGTSAYSLWEDRLF"
     gene            138..>367
                     /locus_tag="ACHOQV_005481"
     CDS             138..>367
                     /locus_tag="ACHOQV_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_302948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HFP9633670.1"
                     /translation="MANNILHSSTSDSESKNTSTDSTLFNEKFPFQLRQDFASRITED
                     ESDFFKWKKIRELAFVSNHKEWNKYDLLILKSV"
BASE COUNT          137 a           51 c           65 g          114 t
ORIGIN      
        1 gttgataaca gcattcaacc cgaagagtca gtttctttca gttatgagtc aattatatgg
       61 gagcattgtt ccgctggcac tagtgcatac agtttatggg aagatcgtct tttttaaaaa
      121 tatctgggat tattataatg gctaacaata ttttacactc ttcaacatca gacagtgaaa
      181 gcaaaaatac aagcactgac agtacgttat ttaatgaaaa attcccattt caactaaggc
      241 aagatttcgc atcaagaata acagaagatg aaagtgattt ttttaaatgg aaaaaaataa
      301 gagagttggc atttgtgagc aatcataaag aatggaataa atatgactta ttaattttaa
      361 aatctgt
//
LOCUS       DAZKGF010000605          363 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZKGF010000605 DAZKGF010000000
VERSION     DAZKGF010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.609"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..354
                     /locus_tag="ACHOQV_005482"
     CDS             <1..354
                     /locus_tag="ACHOQV_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633671.1"
                     /translation="ISPAEKLDKWLRENIIRVKGYEYQKSHILQYGPYALRNKCVLDE
                     ALDILAEQKKIVIDYSIGQKIIYIGDAITPCELANEANIPIMERGMFMVCWDHKLNKY
                     RNEEQTKIWNITTKN"
BASE COUNT          144 a           59 c           71 g           89 t
ORIGIN      
        1 atatctcctg cagaaaaact ggataaatgg ctcagagaaa atatcatccg ggtaaaagga
       61 tacgaatatc aaaaaagcca tatactacaa tatggtccat atgcgctcag gaataaatgt
      121 gttcttgatg aagcacttga tatacttgca gaacagaaaa aaatcgtaat tgattattca
      181 atcggccaaa aaatcattta tatcggggac gcgattacac cttgtgaatt agcaaatgag
      241 gcaaacatcc cgataatgga gcgtggaatg tttatggtct gttgggacca taagctgaat
      301 aagtacagaa atgaagaaca gactaaaata tggaacataa ctacaaaaaa ttaaagttcc
      361 cgt
//
LOCUS       DAZKGF010000606          351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZKGF010000606 DAZKGF010000000
VERSION     DAZKGF010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.610"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          140 a           73 c           41 g           97 t
ORIGIN      
        1 agtaaaggcg cattatatga atttaacaat ccagatcaac tgaaaatacc tcaacctcat
       61 aaacacatag cgtcaacatt caatgacata atgagtaaag atgttggtta tgcatacacg
      121 tcattactct atgcctatcc cttaacaacc tgcctcttaa gactgaatcc attcagcaat
      181 agatgaccaa cattataaca atgacaataa aaacaatatt taaaacaatc aataaaatat
      241 cagacacaga caattaaacc ttaaacaaca aatggttaca ttgatttttg ttgatttgcg
      301 ctacgcgttc cccatgaata aagcgtcatt tcaaattagc accaatattt a
//
LOCUS       DAZKGF010000607          351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZKGF010000607 DAZKGF010000000
VERSION     DAZKGF010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.611"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..200)
                     /locus_tag="ACHOQV_005483"
     CDS             complement(<1..200)
                     /locus_tag="ACHOQV_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633672.1"
                     /translation="MSQTALLALDGEGIFMQNMVISPSMQFQEKDQSGQTSSTANAEQ
                     GIKAKELRVTGMITFDNERALQR"
     gene            complement(172..>351)
                     /locus_tag="ACHOQV_005484"
     CDS             complement(172..>351)
                     /locus_tag="ACHOQV_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633673.1"
                     /translation="QKAQAWVFQYRGYHRAAAAAMVKDIPAPTAVHTVAALLAADSLT
                     ELGKMIHEPDRTARP"
BASE COUNT           61 a          106 c          102 g           82 t
ORIGIN      
        1 cgctgaaggg cccgctcatt gtcaaaggtg atcatcccgg ttacgcgaag ctctttggcc
       61 ttgatgccct gctcggcgtt cgcggtgctc gatgtctgac cggactggtc cttttcctgg
      121 aactgcatcg agggcgaaat caccatgttc tgcatgaaaa taccctcacc atcaagggcg
      181 agcagtgcgg tctggctcat ggatcatttt ccccagttcg gttaatgagt ctgccgccag
      241 gagtgcggcc acagtatgta cggcggtcgg tgccgggata tccttcacca tggccgccgc
      301 tgcggcgcgg tgataccccc ggtactgaaa tacccaggcc tgcgccttct g
//
LOCUS       DAZKGF010000608          349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZKGF010000608 DAZKGF010000000
VERSION     DAZKGF010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.612"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..306
                     /locus_tag="ACHOQV_005485"
     CDS             <1..306
                     /locus_tag="ACHOQV_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFP9633674.1"
                     /translation="FDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAG
                     YPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT          121 a           68 c           60 g          100 t
ORIGIN      
        1 tttgatacta tttataccct gaaaaacgaa cttcatgatc gtagtcatcg aaagtatcag
       61 caaaccatta ccgataaaac agtattgatt cactatacag ggataactaa accatggcat
      121 agctgggctg gatatccatc tgcatcatac tttaatatcg cgcgtgaaca atctccctgg
      181 aagaaatatc ctcttaaaga ggcgcggact gttgcagaaa tgcagaaaca atataagcat
      241 ctgtttgccc atggtgagta tattaaaggt ataacttcat taattaagta caagcttaag
      301 aaataaatta ctcttatgat tacaagtata cgctatcgcg gcttctcat
//
LOCUS       DAZKGF010000609          346 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZKGF010000609 DAZKGF010000000
VERSION     DAZKGF010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.613"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>346
                     /locus_tag="ACHOQV_005486"
     CDS             <1..>346
                     /locus_tag="ACHOQV_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9633675.1"
                     /translation="DYITETLTKLEKQTYPNFEIVIVNDGSKDNTSNVLREYGLTHSR
                     LIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNYLLKMYSRQHETGGDAIYC
                     GLYGHHIKNGVTY"
BASE COUNT          118 a           49 c           73 g          106 t
ORIGIN      
        1 tgattacatt actgaaactc taacgaaatt agaaaaacaa acttacccaa attttgaaat
       61 tgttattgtt aatgatggtt ctaaagataa cacatcaaat gttttgagag agtatgggtt
      121 aacccactct cgattaatta ttatcaataa agaaaatggc ggtgtttcgt ctgccaggaa
      181 tacaggtatc cgcaaggcgc aaggacagtt tatatgtttt atggatgatg atgatgagat
      241 agatcctaac tatctgctga agatgtattc cagacaacat gagacgggag gggatgcgat
      301 ttattgtggg ctttatggac atcatataaa aaatggtgtt acttac
//
LOCUS       DAZKGF010000610          344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZKGF010000610 DAZKGF010000000
VERSION     DAZKGF010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.614"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>344
                     /locus_tag="ACHOQV_005487"
     CDS             <1..>344
                     /locus_tag="ACHOQV_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9633676.1"
                     /translation="GIATAQNVGLRISLSLCSKYALLLDQDSEIETDLVSKLLDGYIT
                     LEQKGIKLAAVGPRPFDLFENKKMKANIQRDIQIFEGFSLTKQIIASGKLINLEVLDQ
                     VGFMEDDLFIDG"
BASE COUNT          116 a           52 c           67 g          109 t
ORIGIN      
        1 tggtattgca acggcacaaa atgtcgggct aagaatttct ctatctttat gctcaaagta
       61 tgcgctatta ctggatcaag attcagaaat tgaaacagat ttagtgtcaa aacttctcga
      121 cggttacatt acattagaac aaaaaggcat caaacttgcc gctgttggtc cccgaccgtt
      181 tgatttattc gaaaacaaaa aaatgaaagc aaacattcaa agagatattc aaatatttga
      241 gggtttttct ttgacgaaac agataatcgc ttcaggtaag ctaattaatt tggaagtttt
      301 agatcaagtc ggttttatgg aagatgattt atttattgat ggtg
//
LOCUS       DAZKGF010000611          339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZKGF010000611 DAZKGF010000000
VERSION     DAZKGF010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.615"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            128..334
                     /locus_tag="ACHOQV_005488"
     CDS             128..334
                     /locus_tag="ACHOQV_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFP9633677.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT           94 a           71 c           84 g           90 t
ORIGIN      
        1 caataagcaa aaataagtgg tcggatgagt agtaaaatta cacaaaaggc ggtactattt
       61 tcatcagata aacaaattca tttttccggt gctattcacc gggtagatac aaaaggggga
      121 gaaaagtatg gaactctaca gagaatatcc tgcatggctt atctttttac gccgtactta
      181 tgcggttgca gcgggcgttc tggcgctgcc tttcatgctt ttctggaaag accgcgcccg
      241 cttttacagc tacctgcacc gcgtctggtc gaaaaccagc gataaaccgg tgtggatgga
      301 tcaggccgaa aaggcgactg gtgattttta ctgattgct
//
LOCUS       DAZKGF010000612          337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZKGF010000612 DAZKGF010000000
VERSION     DAZKGF010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.616"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           79 a           90 c           90 g           78 t
ORIGIN      
        1 atgaggtgtt acgtggatat gttgcttatt acaagtacag ctaatataaa aacttgagaa
       61 agagataacg ggttatatgg tggtttatcc ccgcaggcgc ggggaactca tagagaaaag
      121 aaacggcagc tttgccactt ccggtttatc cccgctggcg cggggaactc cgcgtttcta
      181 atctcaccgc cacggcgatc aacggtttat ccccgctggc gcggggaact caccccttta
      241 acgtcaccag accgctaacg acgcggttta tccccgctgg cgcggggaac tcgcgtaacc
      301 attttgatgg cgctaccgaa atggcggttt atccccg
//
LOCUS       DAZKGF010000613          336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZKGF010000613 DAZKGF010000000
VERSION     DAZKGF010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.617"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>336
                     /locus_tag="ACHOQV_005489"
     CDS             <1..>336
                     /locus_tag="ACHOQV_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633678.1"
                     /translation="KQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGY
                     CSAQGEQWEYRYDASGRRTEKRCDRKKIRFTYLWDGDNIAEIREYRDDKLYSVRHLVF
                     NGFELISQQ"
BASE COUNT           88 a           89 c          109 g           50 t
ORIGIN      
        1 gaaacaggcc ggaaacacgc agtatgacta tgacgccgca ggccggatgg tcagccgcac
       61 cagacaccgt gacggctacc gcccggaaac agagcggttc cggtgggaca gccgtgacca
      121 gctgaccggg tattgcagcg cacagggtga gcagtgggaa taccgctacg acgccagcgg
      181 caggcggacg gaaaaacgct gcgaccggaa gaaaatccgt tttacgtacc tgtgggacgg
      241 cgacaatatt gcggaaatcc gggaataccg cgatgataaa ctgtacagcg tacggcacct
      301 ggtgtttaac ggctttgagc tgataagcca gcagtg
//
LOCUS       DAZKGF010000614          324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZKGF010000614 DAZKGF010000000
VERSION     DAZKGF010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.618"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(165..>324)
                     /locus_tag="ACHOQV_005490"
     CDS             complement(165..>324)
                     /locus_tag="ACHOQV_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFP9633679.1"
                     /translation="VKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSD
                     AWNERTTA"
BASE COUNT           79 a           88 c           76 g           81 t
ORIGIN      
        1 ttcactcact gtcagaagac cttcagacac gctaccgccc ttcgccataa cccgggcgca
       61 tcaggtctgt gttcaggatt gagaattctt tcttcagcac ccgccaacag atactgaaga
      121 aaaaagaaaa atgacggaca ggataccctg cccgtccggg agaattacgc agtggtacgt
      181 tcattccacg cgtcagaatg aatgatgtta ccgtccttat aagtccaggt gattttctcg
      241 tagcgcagct ccacctgctc aaggtggtta tgcttctcgt aggcgggatt cttgatgtcg
      301 tgcatgaccg ggttaacctt cacc
//
LOCUS       DAZKGF010000615          322 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZKGF010000615 DAZKGF010000000
VERSION     DAZKGF010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.619"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(197..>322)
                     /locus_tag="ACHOQV_005491"
     CDS             complement(197..>322)
                     /locus_tag="ACHOQV_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9633680.1"
                     /translation="LLITFLPVFLLPVLGNIGFANTTFSDADFGALGILLGIIVR"
BASE COUNT          115 a           70 c           67 g           70 t
ORIGIN      
        1 ccgtcgaacc gtgtgcatga actgctgcac tgaaacttct cagctgtaaa ataatcctta
       61 cgctacgtac ttcccggagc gcttacgatt aatcggatgc agtcctgact ggaagaaaga
      121 aaaatgggaa aaaataagag gaattaatat cctccacgtt cctgaaatcc tggaggatat
      181 taatgtcatc aaaatattag cgaacgataa tccctaacaa aatacccagt gcgccaaagt
      241 cagcatcact gaaggtggtg ttagcaaaac caatgttacc cagcacaggc aataagaata
      301 ctggcaggaa ggtaataagc aa
//
LOCUS       DAZKGF010000616          319 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZKGF010000616 DAZKGF010000000
VERSION     DAZKGF010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.620"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          121 a           60 c           46 g           92 t
ORIGIN      
        1 ataatgcatt ctgagcatat cagagcgcat aaaaggcgtc aattatccat gctccctcac
       61 gaaacagaag ctgaactctt aacacctgct atcggaactc actgaataaa caactaaaaa
      121 ggacattgtg aaaattaaca tcaacagtat gtaaagtgat ttgttttgtc ttaaatgggt
      181 gaatgtaaaa aaatattcta tacaatccac acatccttgt atcatatatc aagtaaacat
      241 ccacagagat aacagcttat ttcaaataag cagattctgt ttgatacttc agaaaaatag
      301 tatatgctcc tgatttact
//
LOCUS       DAZKGF010000617          318 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZKGF010000617 DAZKGF010000000
VERSION     DAZKGF010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.621"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(40..213)
                     /gene="yciZ"
                     /locus_tag="ACHOQV_005492"
     CDS             complement(40..213)
                     /gene="yciZ"
                     /locus_tag="ACHOQV_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFP9633681.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            251..>318
                     /gene="ymiD"
                     /locus_tag="ACHOQV_005493"
     CDS             251..>318
                     /gene="ymiD"
                     /locus_tag="ACHOQV_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFP9633682.1"
                     /translation="MQKLPLKEKCLTATANYHPGIR"
BASE COUNT          104 a           69 c           73 g           72 t
ORIGIN      
        1 ccgggcatcc tcaaatagga aaacctgaag gcacgattgt taaatctccc acggtgcctt
       61 cgggatgtct ggcgtagatt cagcaacctg gcgcaagagt tctgctttaa gtatctcgag
      121 attatggatc gcagagtggt aatcgcctgc cacaaggata tctaacacaa tatcaatacg
      181 ttcggcaacg cgctgggcat caaattcaga catcaaggac atccttatag caaaagtggg
      241 aaaatgaata atgcaaaaat tgccgctaaa agagaagtgt ttaacagcaa cggctaatta
      301 tcatccggga atacgata
//
LOCUS       DAZKGF010000618          317 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZKGF010000618 DAZKGF010000000
VERSION     DAZKGF010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.622"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>317)
                     /locus_tag="ACHOQV_005494"
     CDS             complement(<1..>317)
                     /locus_tag="ACHOQV_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ligase"
                     /protein_id="HFP9633683.1"
                     /translation="KDEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFA"
BASE COUNT          105 a           50 c           71 g           91 t
ORIGIN      
        1 acgcaaagaa tggaaacact aatatggctg cacgcgtttc agttactata acagcacata
       61 agctaagcat gaagttaatt gtatataatg ttgttttatg tctaaagtcg gattttaaaa
      121 tgagaattga tgccagtaaa gaagggaaaa ggatgatgta gcctgctgtt gtagcgtggg
      181 acgctgataa tgcaacacgg tcgacgttta aatagaaatg ttgccacatg gcatgcgcaa
      241 aaaaaagtaa ctgcgttgcc agggatgcga acagaattag tttcccaaat tttattttgg
      301 ttctcatctc atcttta
//
LOCUS       DAZKGF010000619          317 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZKGF010000619 DAZKGF010000000
VERSION     DAZKGF010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.623"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>317
                     /locus_tag="ACHOQV_005495"
     CDS             <1..>317
                     /locus_tag="ACHOQV_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP9633684.1"
                     /translation="EGIVITTVRSRGSRKSRFTLTFEGIPYSETVCWRPALLSRPVIS
                     GSLPARIESHEKNDIYAHLDDQGRYRVKLDFDRNSTERGYAYLWLRLAKPCAGDTYGF
                     HSPL"
BASE COUNT           62 a           88 c           94 g           73 t
ORIGIN      
        1 gaaggcatcg ttatcaccac ggtgcgttcc cgcggttcac gcaaaagtcg ctttacgctg
       61 acgtttgagg ggatccccta cagtgaaacg gtctgctggc gtccggcact gctcagtcgc
      121 ccggtgatat ccggctcgct tccggcgcgg attgagagtc acgagaagaa cgatatctac
      181 gctcatctgg acgatcaggg gcgttaccgg gtgaagctgg actttgaccg caacagtaca
      241 gaacggggtt acgcttatct gtggttgcgt ctggctaaac cctgtgccgg tgatacgtac
      301 ggtttccact caccgct
//
LOCUS       DAZKGF010000620          317 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZKGF010000620 DAZKGF010000000
VERSION     DAZKGF010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.624"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>317
                     /locus_tag="ACHOQV_005496"
     CDS             <1..>317
                     /locus_tag="ACHOQV_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFP9633685.1"
                     /translation="AIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIK
                     ETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFES
                     EVY"
BASE COUNT           80 a           71 c           90 g           76 t
ORIGIN      
        1 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
       61 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      121 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      181 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      241 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      301 gaatctgaag tgtacat
//
LOCUS       DAZKGF010000621          310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZKGF010000621 DAZKGF010000000
VERSION     DAZKGF010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.625"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..264)
                     /locus_tag="ACHOQV_005497"
     CDS             complement(<1..264)
                     /locus_tag="ACHOQV_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFP9633686.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPG"
BASE COUNT           89 a           75 c           85 g           61 t
ORIGIN      
        1 gcccggcagc atgatgtcca ggcgattcac aatctggtga agaggttctt tacgctcaag
       61 cgccatgcca acaatacacc agaccatcat ttcgagggga agacggcgct tgcgtagcgt
      121 tacagtacct gattcggcaa ggcaacgaga gatgagttcg gggtcgaggt aatcccccag
      181 agaagtcagt gggttacgca gagaatcgta acgggatacc agatcaagag cctgtccaat
      241 gtgcataaaa aaatccggaa acaagtgagc gtttccggat tcttacacag ccactggatc
      301 ggtcaactga
//
LOCUS       DAZKGF010000622          308 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZKGF010000622 DAZKGF010000000
VERSION     DAZKGF010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.626"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(45..>308)
                     /locus_tag="ACHOQV_005498"
     CDS             complement(45..>308)
                     /locus_tag="ACHOQV_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2590 family protein"
                     /protein_id="HFP9633687.1"
                     /translation="NRVSIGQDVVHSIIESGIAALLIGERSPTLRSDIITRLILLVED
                     DERLVPGTVFINEEAPGRLHVTAETQDFGRINTRVNYERETGR"
BASE COUNT           57 a           97 c           75 g           79 t
ORIGIN      
        1 cttctgtttc cggcataccg ctttcacgga atacctgatt aaaatcaacg tccggtttcc
       61 ctttcatagt tcaccctggt gtttatgcgt ccaaaatcct gtgtctctgc cgtcacatgc
      121 agcctgcccg gtgcctcttc gttaatgaac accgtccccg gaaccagccg ttcatcatcc
      181 tcaaccagga ggataagccg ggtaatgatg tcgctgcgca gcgtcgggct gcgttcgccg
      241 ataagcaggg cggcgatccc gctttcgata atgctgtgca ccacgtcctg gccgatgctg
      301 acccggtt
//
LOCUS       DAZKGF010000623          308 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZKGF010000623 DAZKGF010000000
VERSION     DAZKGF010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.627"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           83 a           61 c           67 g           97 t
ORIGIN      
        1 taattccctt gtgtagatgt acagaattgt acgatttcgg acaccaaaac cgctgttgtc
       61 ccgccattca ggcgatccct gaaagtcatt tggctaaaaa acagtcttaa cgtggtttat
      121 tttctgaatt ccaggcggac cccagtaagg gggagctgag agcgttcaat gaatagatat
      181 aatttattga ttaataaata attggctatt tttattaacc tcttaatggc gtttactgtt
      241 tcattgctcc ccaaacccct atatacaact gtcgctgtgt ggcgtggccg gagaagtagt
      301 gattggta
//
LOCUS       DAZKGF010000624          304 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZKGF010000624 DAZKGF010000000
VERSION     DAZKGF010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.628"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           93 a           63 c           58 g           90 t
ORIGIN      
        1 ccagagtcat aaaatagagt gtctgttgat ctcattttca tattcctttc agactgaagt
       61 gcgttttcat cacccgatga gaaagaactg aagtgatgtt tgccatagat tgcgagatgc
      121 cctgtctcat tgactatcgc ggtgcacctt tatacctgtt ataccagatc aaaaatcacg
      181 caatccatac aacaaaacga gatttgcaat tcgtgtcaca aaatatgtcg atctttttct
      241 aagaggcaga tgccatgtga agccagacga acacttgcgg tggtcttcaa aaattaaaga
      301 tttt
//
LOCUS       DAZKGF010000625          303 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZKGF010000625 DAZKGF010000000
VERSION     DAZKGF010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.629"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..301)
                     /locus_tag="ACHOQV_005499"
     CDS             complement(<1..301)
                     /locus_tag="ACHOQV_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633688.1"
                     /translation="MQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAARHVTTYGDFS
                     FTVDDRYRVDVKGDGFGLFHINGTDSTAISWSSSEMTLVLEMTNHSVAVKGIYTAE"
BASE COUNT           70 a           80 c           81 g           72 t
ORIGIN      
        1 cttctgcggt gtatatacct ttcacggcca cgctgtggtt tgtcatttcc agaacaagcg
       61 tcatctctga cgacgaccaa ctgatggcgg tgctgtcggt tccgttgatg tgaaataaac
      121 caaaaccatc acctttcacg tccacgcggt aacggtcgtc aacggtgaag gagaaatccc
      181 cgtatgtggt gacgtgtctg gcggcctgtt tacaggcgcg gcggtaatag tcgtgccagt
      241 cgttcaggcg ggaagccagc cggggatcca ctgcccacat ccagttaaaa aaatcctgaa
      301 tat
//
LOCUS       DAZKGF010000626          302 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZKGF010000626 DAZKGF010000000
VERSION     DAZKGF010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.630"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..254
                     /locus_tag="ACHOQV_005500"
     CDS             <1..254
                     /locus_tag="ACHOQV_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFP9633689.1"
                     /translation="FNSFKFWFNDNIYISAIVTSVIFSALHLQYTDFRTFLMLFLISL
                     VLISAKIKSNGLLMPILLHMAMNAVITGIQYTLQYHITV"
BASE COUNT           99 a           52 c           42 g          109 t
ORIGIN      
        1 tttttaactc ttttaaattc tggtttaatg acaatattta catctctgct attgtaacat
       61 cagtcatatt ttctgcatta catctacaat atactgactt tcgtacattt ttgatgctct
      121 ttctgatatc gttagttctt atcagtgcaa aaataaagtc gaatggcctg ttaatgccca
      181 tattacttca catggcgatg aatgcagtca taacaggaat acaatatacg ttacaatatc
      241 acataacagt ctgaaattgt tattatgaga tatctataac acctgaagtt ggaaaaagaa
      301 gc
//
LOCUS       DAZKGF010000627          300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZKGF010000627 DAZKGF010000000
VERSION     DAZKGF010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.631"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            127..>300
                     /locus_tag="ACHOQV_005501"
     CDS             127..>300
                     /locus_tag="ACHOQV_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633690.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVL"
BASE COUNT           64 a           76 c           88 g           72 t
ORIGIN      
        1 tcatggtgat ataaatattt tcctaattgt tcttatctga cagatatctc acttaaggct
       61 ttcttataaa tctgtagggt ttcgcctgtc agcagacaaa taacccgata aaacaaggat
      121 gagcagatga gcggaaaacc ggcggcgcgt cagggcgaca tgacgcagta tggcggtagc
      181 attgtccagg gttcagccgg ggtgcgcatc ggtgccccca ccggcgtggc ctgttcggtg
      241 tgccccggcg gggtgacgtc cggccatccg gtcaatccgc tgctcggtgc gaaggtcctt
//
LOCUS       DAZKGF010000628          300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZKGF010000628 DAZKGF010000000
VERSION     DAZKGF010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.632"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           73 a           73 c           69 g           85 t
ORIGIN      
        1 tgattgcttc ccgtttccca atacggcggc gcggggagtc ataaaggtaa gaaatttcca
       61 gcagtacggc gtcctattgt tcacggcggt aatgcaccag ccagtattgg ctatcgtagt
      121 gataaccgtg taacctgccg ccccatcgcg tatctaattt tcattctttt ccataaaccg
      181 tccgtacaca ttgtgatagt aaaaatacaa tacatcttcg ctgatgcggt tattctgctg
      241 catgtgggga gcgccgaata ctgctcccgg tatggacagt aattactatt tagtacgacg
//
LOCUS       DAZKGF010000629          298 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZKGF010000629 DAZKGF010000000
VERSION     DAZKGF010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.633"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>298)
                     /locus_tag="ACHOQV_005502"
     CDS             complement(<1..>298)
                     /locus_tag="ACHOQV_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633691.1"
                     /translation="LPPVEWLTYGSGYLAGMKLGGTPLLEYMRDRLHRETVRSFGSMA
                     GNTAAYELTSTYTPAGQLQSRHLNSLVFDCDYNWNDNGDLMRISGPRQTREYGY"
BASE COUNT           47 a          100 c           85 g           66 t
ORIGIN      
        1 ctgtagccgt attcccgcgt ctgtcgcggg ccgctgatgc gcatcaggtc gccgttgtca
       61 ttccagttat agtcacaatc aaacaccagg ctgttcaggt gccggctctg taactggcct
      121 gcgggggtgt aagtgctggt cagttcatat gcggctgtat tgcctgccat gctgccgaag
      181 ctgcgcaccg tctcccggtg cagtctgtcc cgcatgtact ccagcagcgg cgtcccgccc
      241 agcttcatgc ccgccagata accgctgcca taggtcagcc actccaccgg cggcaggc
//
LOCUS       DAZKGF010000630          296 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZKGF010000630 DAZKGF010000000
VERSION     DAZKGF010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.634"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>296
                     /locus_tag="ACHOQV_005503"
     CDS             <1..>296
                     /locus_tag="ACHOQV_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633692.1"
                     /translation="RYHYDLTGKLTQSEDEGLVILWYYDESDRITHRTVNGEPAEQWQ
                     YDGHGWLREISHLSEGHRVAVHYGYDDKGRLTGERQTVENPETGELLWQHETKQ"
BASE COUNT           77 a           75 c           96 g           48 t
ORIGIN      
        1 aacgttatca ttatgacctg accggaaaac tcacgcagag tgaagatgag gggcttgtca
       61 tcctctggta ctacgatgaa tcggaccgta tcactcaccg cacggtgaac ggcgaaccgg
      121 cagagcagtg gcagtatgat ggccacggct ggctgagaga aatcagccac ctgagcgaag
      181 gccaccgtgt tgccgtccac tatggctatg acgataaggg ccgtctgacc ggggagcgcc
      241 agacggtgga gaacccggag acgggggaac tgctgtggca gcatgagacg aaacag
//
LOCUS       DAZKGF010000631          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZKGF010000631 DAZKGF010000000
VERSION     DAZKGF010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.636"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>291
                     /locus_tag="ACHOQV_005504"
     CDS             <1..>291
                     /locus_tag="ACHOQV_005504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9633693.1"
                     /translation="IGTHFIVEKDGNIYQAASLFVYTSHVGKIKSRCKEEKTWSSEEK
                     KKTEAFGWNAKKIHDHEKIKQYPYRYPYNMDSVGIEVIALYDKKNKKWESATD"
BASE COUNT          118 a           35 c           62 g           76 t
ORIGIN      
        1 attggaactc attttattgt tgaaaaagat ggtaatatat accaggctgc aagcctattt
       61 gtatatacca gccatgtggg gaagataaag tctcgatgta aggaggaaaa aacatggtcg
      121 tctgaagaaa aaaagaaaac agaggcattc ggatggaatg ctaagaagat acatgatcat
      181 gaaaaaatta aacaatatcc ttatagatat ccatataata tggacagtgt tggcattgag
      241 gttattgcat tatatgataa aaaaaataaa aaatgggagt ctgctacaga c
//
LOCUS       DAZKGF010000632          289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZKGF010000632 DAZKGF010000000
VERSION     DAZKGF010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.637"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..>289
                     /locus_tag="ACHOQV_005505"
     CDS             14..>289
                     /locus_tag="ACHOQV_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFP9633694.1"
                     /translation="MSWTYDAAKGVGRIQQDDQQFVMHGNLNGNLNAGKNLYFTGENG
                     IIDLKDNVNQGAGYLQFADDYTVTTSNDSSWSGGGIIVNYGTTVKWGI"
BASE COUNT           95 a           42 c           72 g           80 t
ORIGIN      
        1 cagtaaagaa catatgtcat ggacatatga tgcagccaaa ggcgtgggcc gtattcagca
       61 ggatgaccaa cagtttgtga tgcatggtaa cctgaatggt aatctgaatg caggtaaaaa
      121 tctttatttt acaggcgaaa atggcattat tgatttaaaa gataatgtta atcagggggc
      181 cggatatctt cagtttgctg atgattatac agtaacaacg agcaatgaca gttcatggtc
      241 cggaggaggc attattgtca attatggtac aactgtaaaa tggggtata
//
LOCUS       DAZKGF010000633          289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZKGF010000633 DAZKGF010000000
VERSION     DAZKGF010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.638"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>289
                     /locus_tag="ACHOQV_005506"
     CDS             <1..>289
                     /locus_tag="ACHOQV_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633695.1"
                     /translation="GNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGS
                     DTLKRRITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSG"
BASE COUNT           88 a           72 c           69 g           60 t
ORIGIN      
        1 tcggtaatgc caccagaatt aacggtgatt catctgttgc cataggtctg acatcgtccg
       61 taaccgggaa cgactccgtg gcactgggca gtcattccag tgctaacgga aacaatatcg
      121 tatccgtcgg ctctgacaca ctgaaacgcc gcatcacaaa tctggcacag gggaaagatg
      181 accatgatgc ggtaaatctg atgcagatga ccagtgctgt taaccgggaa gctggagaaa
      241 gaataaaatc aacaaacaac ctgcagaatc agcttaatac cctctctgg
//
LOCUS       DAZKGF010000634          285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZKGF010000634 DAZKGF010000000
VERSION     DAZKGF010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.639"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          100 a           57 c           50 g           78 t
ORIGIN      
        1 tcacataaaa aagaggttac cgccttataa tgataagagt tcattatcca ttccatcccg
       61 acaaaacctg cctgtgtata aaaaataaga accatgtcac aaaacattac attccggcaa
      121 gttatgacag aattactgaa aagcgttatc tgtagtaatt gtagtttctg atatatgctc
      181 atccatccgg aaatacgaca tggtcctgac tggcgttcac ttcaaagtgt aatttattat
      241 tcacacactg agactgaaaa acagatggcg tggatgagta cacag
//
LOCUS       DAZKGF010000635          282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZKGF010000635 DAZKGF010000000
VERSION     DAZKGF010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.640"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>282
                     /locus_tag="ACHOQV_005507"
     CDS             <1..>282
                     /locus_tag="ACHOQV_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633696.1"
                     /translation="ALNNDNGLISAAAGTGRIKTQQAVSNTEGRMESAGRLDISAGNL
                     NNHQGTVVADGLSVTLDGALDNTSGRLLSQKTLSVSGSELVSDDGLIQSG"
BASE COUNT           68 a           62 c           92 g           60 t
ORIGIN      
        1 ggcgctgaat aacgacaacg gactgatttc agctgctgcc ggaacgggca ggataaaaac
       61 acagcaggct gtcagcaata ctgagggacg aatggagtct gctggcaggc tggatatttc
      121 ggcagggaat ctgaataacc accagggtac ggtagtggct gacgggctgt ctgtgacgct
      181 ggatggtgcg ctggataata cgtccggcag actgctttca cagaaaacgc tgtctgtgtc
      241 aggcagcgaa ctggtctctg atgatggtct gattcagtcc gg
//
LOCUS       DAZKGF010000636          278 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZKGF010000636 DAZKGF010000000
VERSION     DAZKGF010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.641"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..100
                     /locus_tag="ACHOQV_005508"
     tRNA            24..100
                     /locus_tag="ACHOQV_005508"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHOQV_005509"
     tRNA            143..218
                     /locus_tag="ACHOQV_005509"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZKGF010000637          274 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZKGF010000637 DAZKGF010000000
VERSION     DAZKGF010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.642"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            78..>274
                     /locus_tag="ACHOQV_005510"
     CDS             78..>274
                     /locus_tag="ACHOQV_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFP9633697.1"
                     /translation="MSATPSPLRYPGGKAAIQDMISAIICSNNLTGGHYAEPYAGGGG
                     LALSLLFKQYIHEIHLNDLDRS"
BASE COUNT           88 a           58 c           48 g           80 t
ORIGIN      
        1 tcaacagtag ctctgtccat aagtaatcaa ctgttctaaa ataaatcggt atgaatctat
       61 tttcacaggg aaagactatg tcagcaactc catcaccatt acgatatccg ggagggaaag
      121 ccgcaattca agatatgatt tcggctataa tttgctccaa caaccttact ggcggacact
      181 atgcagaacc ctacgctgga ggtggtggtc ttgctttatc attattattc aaacaatata
      241 tacatgaaat acatttaaat gatcttgatc gctc
//
LOCUS       DAZKGF010000638          269 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZKGF010000638 DAZKGF010000000
VERSION     DAZKGF010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.643"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>269
                     /locus_tag="ACHOQV_005511"
     CDS             <1..>269
                     /locus_tag="ACHOQV_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFP9633698.1"
                     /translation="TLFETITLFDDFSADDMQYGDMEEQDFLSLGLSDISAKVDPYRL
                     IKYHFPGPNSTYGAFSVPTSGTKISQSECIDILFAEMKDLAKMFS"
BASE COUNT           79 a           49 c           46 g           95 t
ORIGIN      
        1 tacacttttc gaaactatta ctttattcga tgatttttct gctgatgata tgcaatatgg
       61 ggatatggaa gaacaagatt ttctctcact cggcttgagc gacatctctg caaaagttga
      121 tccttaccgc ctcattaaat accattttcc tgggccgaat tctacttatg gtgcttttag
      181 tgttccaact tctggaacaa aaatatcaca aagtgaatgt attgatattt tatttgcaga
      241 aatgaaagat ctggcaaaaa tgttttcct
//
LOCUS       DAZKGF010000639          267 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZKGF010000639 DAZKGF010000000
VERSION     DAZKGF010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.644"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..>267
                     /locus_tag="ACHOQV_005512"
     CDS             38..>267
                     /locus_tag="ACHOQV_005512"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HFP9633699.1"
                     /translation="MIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDK
                     SGVITLQKDVYSYDIAKDKWEKLMTRPPVSLAG"
BASE COUNT           98 a           41 c           62 g           66 t
ORIGIN      
        1 atatatagga ctgggaagtc tgggaaataa ctggtatatg atagatacaa accagagtga
       61 aaaaaaatgg actaagatag cacaatggcc gacagttccc agagagcagg caacagctac
      121 tattattgat ggtaagatct atgtatttgg aggaattgga aaagataaaa gtggggtcat
      181 aaccttacaa aaagatgtct attcctatga tattgctaaa gataaatggg aaaaattgat
      241 gacccgtccc cctgtatctc ttgccgg
//
LOCUS       DAZKGF010000640          266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZKGF010000640 DAZKGF010000000
VERSION     DAZKGF010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.645"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..243
                     /locus_tag="ACHOQV_005513"
     rRNA            <1..243
                     /locus_tag="ACHOQV_005513"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           65 a           63 c           76 g           62 t
ORIGIN      
        1 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
       61 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      121 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      181 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcacctcc
      241 ttaccttaaa gaagcgtact ttgcag
//
LOCUS       DAZKGF010000641          263 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZKGF010000641 DAZKGF010000000
VERSION     DAZKGF010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.646"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>263)
                     /locus_tag="ACHOQV_005514"
     CDS             complement(<1..>263)
                     /locus_tag="ACHOQV_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP9633700.1"
                     /translation="RAYDERDRLVSETSRSGDVIRYAYDNPHSELPATTTDATGSTRQ
                     MTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGVSLYR"
BASE COUNT           47 a           87 c           74 g           55 t
ORIGIN      
        1 gcggtaaaga ctgacacctt cctcacggtg gaccgccgtc atctggccga agcggtcgta
       61 ttcatagcgg gtctggtagc ccgagcagtc ggtgaacgcc agcaactgac cgtagcggct
      121 ccacgtcatc tgccgggtgc tgcccgtcgc atctgttgtc gtggccggta actcactgtg
      181 cggattatca taagcatacc ggatgacgtc cccactgcgc gatgtctccg ataccagcct
      241 gtcccgctca tcatacgccc ggc
//
LOCUS       DAZKGF010000642          263 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZKGF010000642 DAZKGF010000000
VERSION     DAZKGF010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.647"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>263)
                     /locus_tag="ACHOQV_005515"
     CDS             complement(<1..>263)
                     /locus_tag="ACHOQV_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633701.1"
                     /translation="RAYDELDRLVSETSRSGETVRYRYDDVHSELPATTTDATGSTRQ
                     MTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGVSLYR"
BASE COUNT           42 a           87 c           77 g           57 t
ORIGIN      
        1 gcggtaaaga ctgacacctt cctcacggtg gaccgccgtc atctggccga agcggtcgta
       61 ttcatagcgg gtctggtagc ccgagcagtc ggtgaaggcc agcaactgcc cgtagcggct
      121 ccaggtcatc tgccgggtgc tgcccgtcgc atccgttgtc gtcgccggta actcactgtg
      181 cacgtcatcg tagcggtagc gtactgtctc cccgctgcgc gatgtctccg ataccagtct
      241 gtccagctca tcatatgccc ggc
//
LOCUS       DAZKGF010000643          261 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZKGF010000643 DAZKGF010000000
VERSION     DAZKGF010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.648"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>261
                     /gene="ompT"
                     /locus_tag="ACHOQV_005516"
     CDS             <1..>261
                     /gene="ompT"
                     /locus_tag="ACHOQV_005516"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP9633702.1"
                     /translation="AIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEH
                     YDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNR"
BASE COUNT           85 a           50 c           58 g           68 t
ORIGIN      
        1 gcaatcggct acaaacaacg ttttaaaatg ccctacattg gcttgactgg aagttatcgt
       61 tatgaagatt ttgaactcgg tggcacattt aaatacagcg gctgggtgga atcatctgat
      121 aacgatgaac actatgaccc gggaaaaaga atcacttatc gcagtaaggt caaagaccaa
      181 aattactatt ctgttgcagt caatgcaggt tattacgtca cacctaacgc aaaagtttat
      241 gttgaaggcg catggaatcg g
//
LOCUS       DAZKGF010000644          258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZKGF010000644 DAZKGF010000000
VERSION     DAZKGF010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.649"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..242)
                     /locus_tag="ACHOQV_005517"
     CDS             complement(<1..242)
                     /locus_tag="ACHOQV_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFP9633703.1"
                     /translation="MQIIIMTRDRYLEYGLMRMLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTD"
BASE COUNT           78 a           67 c           54 g           59 t
ORIGIN      
        1 atatctgtca ggcatctcac agaggaaaca ggaatgacaa gaaaacgacg cccacagaac
       61 atacagtatg taagcctttc cagattacgg tcacagagaa tcacataact gtcttcggga
      121 agtggcagac ggcgctttcc ggcatcaaac agttcacggc ctgtcgtgag cctgtatccg
      181 ttcagcatac gcatcaggcc atattccaga tatctgtcac gggtcataat aataatctgc
      241 atactctcag acccttat
//
LOCUS       DAZKGF010000645          257 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZKGF010000645 DAZKGF010000000
VERSION     DAZKGF010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.650"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>257
                     /locus_tag="ACHOQV_005518"
     CDS             <1..>257
                     /locus_tag="ACHOQV_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633704.1"
                     /translation="AAAVAAATEKVEAAEAADKAAKDKLAELNADKLITPEEKAQLET
                     AKQNADTLKGEADSAVKALPDTAAEKGGLQGRVDALDGIQV"
BASE COUNT           68 a           57 c          102 g           30 t
ORIGIN      
        1 ggcagcagcg gtggcggcgg ccacggagaa agtggaagcg gcagaagcgg cggacaaggc
       61 tgcgaaggac aagcttgcag agctgaatgc agacaagctg atcacgccgg aagagaaggc
      121 gcagctggaa acggcgaagc agaatgctga caccctgaag ggtgaggcag acagtgccgt
      181 gaaggcgttg ccggatacag ccgcggagaa aggtggcctg cagggtcgtg tggatgcact
      241 ggatggtatc caggtac
//
LOCUS       DAZKGF010000646          252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZKGF010000646 DAZKGF010000000
VERSION     DAZKGF010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.651"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>252)
                     /locus_tag="ACHOQV_005519"
     CDS             complement(<1..>252)
                     /locus_tag="ACHOQV_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFP9633705.1"
                     /translation="GDLTLQFTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSA
                     LFYHLPLLAELEGEQADGRCWQPGISVPEGVYRFRFRKMP"
BASE COUNT           50 a           92 c           62 g           48 t
ORIGIN      
        1 ggcatcttcc tgaaccggaa ccggtatacg ccctccggta cagatatccc cggctgccag
       61 catctgccgt cggcctgttc gccttccagt tccgccagca acggcaggtg ataaaacaga
      121 gccgaccaga acaccaccat gttccacaca ctcccctgcg cggcctgctc ttctgctgtc
      181 gctcccggtg gaaacattct gcctctggcc agccggacag catacgtcgt gaactgcagg
      241 gtcaggtcac ca
//
LOCUS       DAZKGF010000647          250 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZKGF010000647 DAZKGF010000000
VERSION     DAZKGF010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.652"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            12..>250
                     /locus_tag="ACHOQV_005520"
     CDS             12..>250
                     /locus_tag="ACHOQV_005520"
                     /inference="COORDINATES: protein motif:HMM:NF014467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9633706.1"
                     /translation="MEDHRGKAVWRLESGSALLITEPGPLPENVTEIKPDGEFCRWDG
                     SSWVKDEAAEQAYRVSEAENKKRKLIQEATNTINIL"
BASE COUNT           76 a           54 c           78 g           42 t
ORIGIN      
        1 cctggcacct catggaagat caccggggta aagccgtctg gcgtctggag tcggggagcg
       61 ccttactgat tactgagccg ggcccgttac cggaaaacgt gacagaaata aaaccggacg
      121 gggagttctg ccggtgggat ggttcatcat gggttaagga tgaagcagca gaacaggctt
      181 accgggtgag cgaggcggag aacaaaaaac ggaaattaat acaggaggca acaaatacaa
      241 taaacatcct
//
LOCUS       DAZKGF010000648          250 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZKGF010000648 DAZKGF010000000
VERSION     DAZKGF010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.653"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            12..>250
                     /locus_tag="ACHOQV_005521"
     CDS             12..>250
                     /locus_tag="ACHOQV_005521"
                     /inference="COORDINATES: protein motif:HMM:NF014467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9633707.1"
                     /translation="MEDHRGKTVWRLESGSALLITEPGPLPENVTIIKPDGEFCRWDG
                     SSWAKDEAAEQAYRVSEAENKKRELIQEATNTINIL"
BASE COUNT           76 a           56 c           77 g           41 t
ORIGIN      
        1 cctggcagct catggaagat caccggggta aaaccgtctg gcgtctggag tcgggaagcg
       61 ccttactgat tactgagccg ggcccgttac cggaaaacgt gaccataata aaaccggacg
      121 gggagttctg ccgctgggac gggtcatcat gggctaagga tgaagcggca gaacaggctt
      181 accgggtgag cgaggcggag aataaaaaac gggaattaat acaggaagca acaaatacaa
      241 taaacatcct
//
LOCUS       DAZKGF010000649          249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZKGF010000649 DAZKGF010000000
VERSION     DAZKGF010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.654"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..204
                     /locus_tag="ACHOQV_005522"
     CDS             <1..204
                     /locus_tag="ACHOQV_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP9633708.1"
                     /translation="EGPFSRGQAVAVTTAYRNVFTEDDQGTYFRLVIRNAEGQLRWRC
                     WNFESDAGKQLNVWLSSEGLLRQ"
BASE COUNT           56 a           65 c           67 g           61 t
ORIGIN      
        1 gaaggccctt tctcacgcgg acaggctgtt gccgtcacaa cagcttaccg caatgtgttt
       61 actgaagatg accagggaac atatttccgg ctggttatcc gtaatgccga agggcagcta
      121 cgctggcggt gctggaattt tgagtctgac gccggaaaac agcttaatgt gtggctctcc
      181 agtgaagggc ttctcaggca ataaacgtct tcatttcatc catcaggccg catcttctcc
      241 gggagacgc
//
LOCUS       DAZKGF010000650          249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZKGF010000650 DAZKGF010000000
VERSION     DAZKGF010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.655"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>249
                     /locus_tag="ACHOQV_005523"
     rRNA            <1..>249
                     /locus_tag="ACHOQV_005523"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           60 a           58 c           77 g           54 t
ORIGIN      
        1 ttccagagat ggattggtgc cttcgggaac tgtgagacag gtgctgcatg gctgtcgtca
       61 gctcgtgttg tgaaatgttg ggttaagtcc cgcaacgagc gcaaccctta tcctttgttg
      121 ccagcggtcc ggccgggaac tcaaaggaga ctgccagtga taaactggag gaaggtgggg
      181 atgacgtcaa gtcatcatgg cccttacgac cagggctaca cacgtgctac aatggcgcat
      241 acaaagaga
//
LOCUS       DAZKGF010000651          244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZKGF010000651 DAZKGF010000000
VERSION     DAZKGF010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.656"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>244
                     /locus_tag="ACHOQV_005524"
     CDS             <1..>244
                     /locus_tag="ACHOQV_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9633709.1"
                     /translation="KVYRIANATAAAINFREGTFSGQIDAQQQTDRLAWQVSFTLRER
                     RSVPEKRQARAAPASSRKQTPQTPGAKGKTVADETPE"
BASE COUNT           71 a           82 c           63 g           28 t
ORIGIN      
        1 aaagtttacc gtatcgcaaa cgccaccgct gccgccatta actttcgtga agggacattt
       61 tccgggcaga tagacgcaca gcagcagacc gaccgcctcg cctggcaggt cagcttcaca
      121 ttacgcgaaa gacgaagcgt accggaaaaa cgccaggcac gcgccgcacc cgccagcagc
      181 cgtaaacaga cacctcagac gcccggcgca aaaggcaaaa ccgtggccga cgaaacaccg
      241 gaga
//
LOCUS       DAZKGF010000652          244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZKGF010000652 DAZKGF010000000
VERSION     DAZKGF010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.657"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(2..>244)
                     /locus_tag="ACHOQV_005525"
     CDS             complement(2..>244)
                     /locus_tag="ACHOQV_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9633710.1"
                     /translation="LSLKRAEAVRDWMRDTGDIPQSCFAVQGYGESRPVAPNDSAEGR
                     ARNRRVEISLVPQADACRLPDASPAPEEGASENITE"
BASE COUNT           45 a           82 c           69 g           48 t
ORIGIN      
        1 atcactccgt tatgttttct gatgcgccct cttccggcgc aggggaagcg tccggcagac
       61 gacaggcatc ggcctgcggt accagactga tttcaacacg gcggttacgc gcgcggcctt
      121 ccgccgagtc attcggggcc acagggcggc tttcaccgta tccctgcacg gcaaaacagc
      181 tttgagggat atcgcccgta tcccgcatcc agtcacgaac cgcctctgca cgcttcaggg
      241 acag
//
LOCUS       DAZKGF010000653          243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZKGF010000653 DAZKGF010000000
VERSION     DAZKGF010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.denovo.658"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>243
                     /locus_tag="ACHOQV_005526"
     CDS             <1..>243
                     /locus_tag="ACHOQV_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9633711.1"
                     /translation="LALENGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAE
                     QPESLAEDPETLSISCEVTFRHGTLRFNGNVSEKLLT"
BASE COUNT           61 a           70 c           60 g           52 t
ORIGIN      
        1 cttgctcttg aaaatggcat taacgccaat ctgttgttca aatggcgaca acaatggcgc
       61 gagggaaagc tgctattacc ttcttcagag agcccccagc tacttcctgt gactctcgat
      121 gcagctgccg aacagccaga atcgctcgca gaggacccgg aaaccctcag tatcagctgt
      181 gaggtaacgt tccggcacgg gacgctccgc ttcaatggca atgtcagcga aaagctcctg
      241 act
//
LOCUS       DAZKGF010000654         3234 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.guided.310, whole genome shotgun sequence.
ACCESSION   DAZKGF010000654 DAZKGF010000000
VERSION     DAZKGF010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.guided.310"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..729)
                     /locus_tag="ACHOQV_005527"
     CDS             complement(<1..729)
                     /locus_tag="ACHOQV_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFP9633712.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELD"
     gene            990..1448
                     /gene="gpt"
                     /locus_tag="ACHOQV_005528"
     CDS             990..1448
                     /gene="gpt"
                     /locus_tag="ACHOQV_005528"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9633713.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            1540..2784
                     /gene="frsA"
                     /locus_tag="ACHOQV_005529"
     CDS             1540..2784
                     /gene="frsA"
                     /locus_tag="ACHOQV_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP9633714.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRYWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            2842..>3234
                     /gene="crl"
                     /locus_tag="ACHOQV_005530"
     CDS             2842..>3234
                     /gene="crl"
                     /locus_tag="ACHOQV_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP9633715.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKL"
BASE COUNT          778 a          793 c          852 g          811 t
ORIGIN      
        1 gtccagttct tctgcatgac ccgccaggaa gcgcaccagc agtttgttgg cattacccag
       61 gccaacgtgg atttcaccgc cggagtgacc gcctttcagg ccttttaagg ttaacttgaa
      121 ggtttcaaag ccagctggaa ccgcttcacg atctaaatgc aggttggagg tgaagtcgat
      181 acctcccgca caacccatgt agatttcacc ttcttcttcg gagtcggtgt taatcagaat
      241 atcagcctgc aaccagttgc tctgtaagcc aaaagcaccg tccataccgg cttcttcggt
      301 catggtcagc agcacttcca gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag
      361 tgcagaggcc ataccaatgc cgttatccgc acccagcgtg gtgccgcgtg ctttaaccca
      421 ttcgccatca atataaggct ggataggatc tttggtgaag tcatgcacgg tgtcgttatt
      481 tttttgcggc accatatcga ggtgggcctg caagacgacc ggtttacgat tttccatacc
      541 tgcggtagca ggtttacgaa tcaggatatt acctacctga tcgcgttcga catggaaacc
      601 tttctctttt gcccaaccaa caatgtattc agcgagttgc tcttcatgat aggacgggtg
      661 aggaatagaa cagattttgg caaaaatatc ccacagcggc tgtggagata attgagacag
      721 ttcagacacg ttaagtctcc ttgtcgatca cccgcaaaac agtattgcag gtcacagggt
      781 tagcagaaaa tgttgtcaac acaagacagg cttgcgagat atgtttgaga ataccacttt
      841 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
      901 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
      961 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     1021 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     1081 gcattattgc cgtaagccgt ggcggtctgg taccgggtgc gttactggcg cgtgaactgg
     1141 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     1201 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     1261 tagataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     1321 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ttatgttgtt gatatcccgc
     1381 aaaatacctg gatcgaacag ccgtgggata tgggcgtcgt attcgtgccg ccaatctccg
     1441 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg
     1501 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
     1561 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
     1621 agtcacggcg cacaactgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg
     1681 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccccg tgaaatcctc
     1741 gacgtccagg cgcgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat
     1801 acggtgattg gctaccgcgg cggcaactgg atttatgagt gggctaccca ggcgatggtg
     1861 tggcaacaaa aagcctgtgc ggaagaagat ccgcaactca gcggtcgata ctggctgcat
     1921 gcggctacgt tgtacaacat tgctgcctat cctcatctga aaggagatga cctggccgag
     1981 caagcgcagg ctttgtcaaa ccgcgcctat gaagaggccg ctcagcgtct gccgggcacg
     2041 atgcggcaga tggagtttac cgtacccggc ggtgcgccca tcaccggctt tttgcatatg
     2101 ccgaaaggcg atggcccgtt cccgacagta ttaatgtgtg gtggtctgga tgcgatgcag
     2161 acggactatt acagcctgta tgaacgttat tttgcgccgc gcggcattgc tatgctgact
     2221 attgatatgc cgtcggtggg cttttcttca aaatggaagc tcacccagga ctccagcctg
     2281 ttgcatcagc acgttttaaa ggcgctgcct aacgtaccgt gggtggatca cactcgcgtc
     2341 gcggcctttg gtttccgttt cggcgctaac gttgccgtgc gtctggcata ccttgaatcg
     2401 ccgcgtctga aagtggttgc ctgtcttggt ccggtagttc ataccctgtt gagtgatttt
     2461 aagtgccagc aacaggtgcc ggaaatgtat cttgacgttc tggccagtcg tttagggatg
     2521 catgatgctt ccgatgaagc gttgcgagtg gagttgaatc gctattcatt aaaagtgcaa
     2581 ggattgctgg gacgtcgctg cccaacgcca atgttatcag gctactggaa gaacgatccg
     2641 ttcagcccgg aagaggactc acgcttaatc acgtcgtcat ctgctgacgg taaattatta
     2701 gagatcccat ttaacccggt gtatcggaat tttgacaaag gccttcagga aatcacagac
     2761 tggatcgaaa aacgtttgtg ttaaaaattt gctaaatttt accaatttgg taaaacagtt
     2821 gcatcacaac aggagattgc aatgacgtta ccgagtggac acccgaagag cagattgatc
     2881 aaaaaattta ccgcactagg cccgtatatt cgtgaaggta agtgcgaaga taatcgattc
     2941 tttttcgatt gtctggctgt atgcgtcaac gtgaaaccgg caccggaagt gcgtgagttc
     3001 tggggctggt ggatggaact ggaagcgcag gaatcccgtt ttacctacag ttaccagttt
     3061 ggtctgttcg ataaagcagg cgactggacg agtgttcaga taaaagatgc cgaagtggtt
     3121 gaacgactgg agcacaccct gcgtgaattt cacgagaagc tgcgtgagct gctggcgacg
     3181 ctgaatctga agctggaacc ggcagatgat tttcgtgatg aaccggtgaa gtta
//
LOCUS       DAZKGF010000655         1635 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.guided.378, whole genome shotgun sequence.
ACCESSION   DAZKGF010000655 DAZKGF010000000
VERSION     DAZKGF010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.guided.378"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            29..1162
                     /gene="ampC"
                     /locus_tag="ACHOQV_005531"
     CDS             29..1162
                     /gene="ampC"
                     /locus_tag="ACHOQV_005531"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP9633716.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1251..>1635
                     /gene="blc"
                     /locus_tag="ACHOQV_005532"
     CDS             1251..>1635
                     /gene="blc"
                     /locus_tag="ACHOQV_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP9633717.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHA"
BASE COUNT          419 a          420 c          402 g          394 t
ORIGIN      
        1 gggccgtttt gtatggaaac cagaccctat gttcaaaatg acgctctgcg ccttattaat
       61 taccgcctct tgctccacat ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac
      121 aattaccccg cttatagagc aacaaaagat ccccggtata gcggtggcgg taatttatca
      181 gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac
      241 acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg
      301 cgacgctatt gctcgagggg aaatcaagtt aagcgatccc gcgacaaaat actggcctga
      361 acttaccgct aaacaatgga atgggatcac actattacat ctcgcgacct acaccgctgg
      421 cggcctgcca ttgcaggtgc cggatgacgt taaatcctca agcgacttgc tgcgcttcta
      481 tcaaaactgg caacctgcat gggctccagg aacacaacgt ctgtatgcca acgccagtat
      541 tgggttgttc ggtgcactgg cggtgaaacc atccggtttg agctttgaac aggcgatgca
      601 aactcgtgtt ttccagccac tcaaactcac tcatacgtgg attaatgtgc cgtccgcaga
      661 agaaaagaat tacgcctggg gatatcgcga aggtaaggca gtgcatgttt cgccaggggc
      721 gttagatgct gaggcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca
      781 aagcaattta aaaccccttg atatcactga gaaaacgctt caacaaggga tacaactggc
      841 acaatctcgc tactggcaaa ccggcgatat gtatcagggc ctgggctggg aaatgctgga
      901 ctggccggtc aatcctgaca tcatcattaa cggcagtgac aataaaattg cactggcagc
      961 acgccccgta aaagcgatta cgcccccaac tcctgcagta cgcgcatcat gggtacataa
     1021 aacgggggcg accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg
     1081 tatcgtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg ccgcctgtca
     1141 gattctcaac gctctacagt aaaatttcat cgggtccgaa tttttggacc ttttctccgc
     1201 ttttccttgc tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc
     1261 tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tctcctacgc
     1321 cgccccgtgg cgtgaccgta gtgaataatt tcgatgctaa acgctatctc ggtacctggt
     1381 atgagattgc ccgttttgat caccgttttg aacgtggact ggaaaaagtc accgcaacat
     1441 acagcctgcg tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag
     1501 aaatgtggca gcagagtgaa gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc
     1561 tgaaagtgtc attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg
     1621 aataccgcca tgcgc
//
LOCUS       DAZKGF010000656         1540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.guided.385, whole genome shotgun sequence.
ACCESSION   DAZKGF010000656 DAZKGF010000000
VERSION     DAZKGF010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.guided.385"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            73..198
                     /locus_tag="ACHOQV_005533"
                     /pseudo
     CDS             73..198
                     /locus_tag="ACHOQV_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            239..314
                     /locus_tag="ACHOQV_005534"
     tRNA            239..314
                     /locus_tag="ACHOQV_005534"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:cat)
     gene            322..398
                     /locus_tag="ACHOQV_005535"
     tRNA            322..398
                     /locus_tag="ACHOQV_005535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Arg,seq:tcg)
     gene            412..488
                     /locus_tag="ACHOQV_005536"
     tRNA            412..488
                     /locus_tag="ACHOQV_005536"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:446..448,aa:Arg,seq:tct)
     gene            580..1539
                     /gene="stxA2b"
                     /locus_tag="ACHOQV_005537"
     CDS             580..1539
                     /gene="stxA2b"
                     /locus_tag="ACHOQV_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFP9633718.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
BASE COUNT          380 a          353 c          388 g          419 t
ORIGIN      
        1 taaccctgta acgaagtttg cgtaacagca ttttgctcta cgagtttgcc agcctccccc
       61 agtggctggc ttttttatgt ccgtagcgtc aaagcagcaa tggcgctggg gcgtcgtgca
      121 actggcgttg agctggagac tgaacgtttt gagcagacgg tcagggaagt tcaggattta
      181 gccagtcaga acggatgata ttgcaggatt agttacgtac cgttattatc ctgcgcccgg
      241 ccctttagct cagtggtgag agcgagcgac tcataatcgc caggtcgctg gttcaaatcc
      301 agcaagggcc accatcacat accgccatta gctcatcggg acagagcgcc agccttcgaa
      361 gctggttgcg cggggttcga gtcctcgatg gcggtccatt atctgtacac tgcgttgtta
      421 gctcagccgg acagagcaat tgccttctaa gcaatcggtc actggttcga atccagtaca
      481 acgcgccata cttatttttt ctggctcgct tttgcgggcc ttttttatat ctgcgccggg
      541 tctggtgctg attacttcag ccaaaaggaa tacctgtata tgaagtgtat attgttaaaa
      601 tgggtactgt gcctgttact gggcttttct tcggtatcct attcccggga atttacgata
      661 gacttttcga cccaacaaag ttatgtctct tcgttaaata gtatacggac agaaatatcg
      721 accccacttg aacatatatc tcaggggacc acatcggtgt ctgttattaa ccacacccca
      781 ccgggcagtt attttgctgt ggatatacga gggcttgatg tctatcaggc gcgttttgac
      841 catcttcgtc tgattattga gcaaaataat ttatatgtgg ccggattcgt taatacggca
      901 acaaatactt tctacaggtt ttcagatttt gcacatatat cagtgcccgg tgtgacaact
      961 gtttccatga caacggacag cagttatacc actctgcagc gtgtcgcagc gctggaacgt
     1021 tccggaatgc aaatcagtcg tcactcactg gtttcatcat atctggcgtt aatggagttt
     1081 agtggaaatg ccatgaccag agatgcatcc agagcagttc tgcgttttgt cactgtcaca
     1141 gcagaagcct tacggttcag gcaaatacag agagaatttc gtctggcact gtctgaaact
     1201 gctcctgttt atacgatgac accggaagaa gtggacctca cactgaactg ggggagaatc
     1261 agcaatgtgc ttccggagtt tcggggagag ggtggtgtca gagtggggcg aatatccttt
     1321 aataatatat cagcgatact gggcacagtg gcggttatac tgaattgcca tcatcagggg
     1381 gcgcgttccg ttcgcgccgt gaatgaagag atacaaccag aatgtcagat aactggcgac
     1441 aggccagtta taaggataaa caatacgtta tgggaaagta ataccgcagc tgcttttctg
     1501 aatcgcaggg ctcactcttt aaatacatcc ggagaataac
//
LOCUS       DAZKGF010000657          372 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303H6797
            SAMN43283665-rid23084423.guided.607, whole genome shotgun sequence.
ACCESSION   DAZKGF010000657 DAZKGF010000000
VERSION     DAZKGF010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283665
            Sequence Read Archive: SRR30325500
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351762.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351762.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:50:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,284
            CDSs (with protein)               :: 5,284
            Genes (RNA)                       :: 88
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 113 of 166
            Pseudo Genes (internal stop)      :: 43 of 166
            Pseudo Genes (multiple problems)  :: 46 of 166
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283665-rid23084423.guided.607"
                     /serotype="O146:H28"
                     /isolate="2303H6797"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..274
                     /gene="stxB2b"
                     /locus_tag="ACHOQV_005538"
     CDS             11..274
                     /gene="stxB2b"
                     /locus_tag="ACHOQV_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFP9633719.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          115 a           59 c           91 g          107 t
ORIGIN      
        1 ggagttaaat atgaagaaga tatttgtagc ggctttattt gcttttgttt ctgttaatgc
       61 aatggcagct gattgtgcaa aaggtaaaat tgagttctct aagtataatg agaatgatac
      121 attcacagta aaagtggccg ggaaagagta ctggactaac cgctggaatc tgcaaccgct
      181 actgcaaagc gcacagttaa caggaatgac ggtaacaatc aaatcaaata cctgtgcgtc
      241 aggttcagga tttgctgaag tgcagtttaa ttaatatcag aagcattgct ggtttcgtgg
      301 tgtgcagcaa tgtagttaca gtgtaatcaa tgtcacaatt cagtcagttg aaggttgtct
      361 gcccgactga ga
//
